LOCUS       DASUGA010000001         2626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1110878, whole genome shotgun sequence.
ACCESSION   DASUGA010000001 DASUGA010000000
VERSION     DASUGA010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110878"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VFX21_00005"
     CDS             complement(<1..393)
                     /locus_tag="VFX21_00005"
                     /inference="COORDINATES: protein motif:HMM:NF025215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HEX5094354.1"
                     /translation="MRLRLFAGAALIMVLLAACSDSGAERTNGVITGAGNLSVHDLRP
                     GDCLDRPDKDSEEIDKVKGVPCDEKHAEEIFAKIIYTGGDAYPDAKAMEKYATARCEA
                     AYRPYVGIDYNASKLLLTYLAPSQDGWEK"
     gene            456..527
                     /locus_tag="VFX21_00010"
     tRNA            456..527
                     /locus_tag="VFX21_00010"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:489..491,aa:Arg,seq:ccg)
     gene            complement(588..1565)
                     /locus_tag="VFX21_00015"
     CDS             complement(588..1565)
                     /locus_tag="VFX21_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEX5094355.1"
                     /translation="MPPARQPGIVWAWPAALAGAAFAIPAALAMPVSVDKGLALGFGV
                     LPAAIVGIAPRRRDRVRIVLMGVLMGVPLAVGASLASWPVLAVASVFVLAVGAAQLST
                     KRPVGRIALSLSVPLVGVGLSYNAGKGAGVALLIVAGSVFAMTVAMLWPQHDAPEHVR
                     VVVPAESMLGYGVRLGLAGGTAAAIGFWFEWEHVGWAAAAAMLVMRPSIEMQELRSWG
                     RVASVIIGGLAAAGLAHATTAPGWYSLAIIVVIAGAGATYASRWYVTPTFTTFIAISL
                     LVYANTDTAQSRFNERVGETLLGVALAYFFGLLVPRVFGRGRPRATLPR"
     gene            1665..2012
                     /locus_tag="VFX21_00020"
     CDS             1665..2012
                     /locus_tag="VFX21_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010568550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX5094356.1"
                     /translation="MRYALLICTDEAASAAWSPEEQAASTAAYMAWGEEMGKRGLLTG
                     GERLRPITEATTVRVRDGEVLTTDGPFAETKEQVGGFYLVDCKDLDEAIDIAAKIPGA
                     AVGSIEVRPIWEM"
     gene            2000..>2626
                     /locus_tag="VFX21_00025"
     CDS             2000..>2626
                     /locus_tag="VFX21_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014467148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5094357.1"
                     /translation="MGDVEAAVAAAFQSEWGRVVATLIRTTGDWDLAEECAQDAFAKA
                     LERWPREGVPRSPGAWLTTTARNRAFDVLRRAAVGRNKTQEAAVLPGEPDADDDDSGV
                     TDDRLRLIFTCCHPALGLEAQVALTLRTLAGLTTDEIARAFLVPEATMAQRLVRAKKK
                     IRNAGIPYRVPPAHLLPERTVAVLGVLYLLFNEGYAATSGENLIRQNLS"
BASE COUNT          470 a          905 c          866 g          385 t
ORIGIN      
        1 cttctcccac ccatcttgcg acggtgcgag gtacgtgagc aacagcttgg acgcgttgta
       61 gtcgatgccg acgtacggcc ggtaggccgc ctcgcagcgc gccgtcgcgt acttttccat
      121 cgccttggca tcgggatagg cgtcaccgcc cgtgtagatg atcttggcga agatctcctc
      181 ggcgtgtttt tcgtcgcacg gcacgccctt caccttgtcg atctcttcgc tgtctttgtc
      241 gggccggtcg aggcagtcgc ccggacgcaa gtcgtgcacg gagaggtttc cggcgccggt
      301 gatcacgccg ttggtgcgct cggcgccgct gtcggaacag gcggcgagca ggaccatgat
      361 gagggcagca cccgcgaaga gtcggagacg catctcggcg agtgtgtcag accggaagcc
      421 ccggttcggc gcacactacg atcagcggtt cccgagcccc cgtagctcag gggatagagc
      481 atcggcctcc ggagccgtgt gcgcaggttc gaatcctgcc gggggcgcat ttttcggttc
      541 ttggtcacgc cgtgtaccga atccgtacac aaatggacca agaacggcta gcgcggaagt
      601 gtcgcacgcg gacggccccg gccgaagaca cgcggcacga gcagcccgaa gaagtacgcc
      661 agcgcgacgc ccaacagcgt ttcgccgact cgttcgttga agcgcgattg cgcggtgtca
      721 gtgttggcgt acacgagcag tgagatcgcg atgaaggttg tgaaggtcgg cgtgacgtac
      781 caccgacttg cgtacgtcgc gcccgctccc gcgatgacaa cgatgatcgc gagtgagtac
      841 caccccggcg cggtcgtcgc gtgagccaga ccggcggccg caagcccgcc gatgatcacc
      901 gacgcgaccc gtccccagct ccgtagctct tgcatctcga tcgacggccg catcacgagc
      961 atcgcggccg cggcggccca acccacgtgt tcccactcga accagaatcc gatcgcggca
     1021 gccgttccgc ctgcgagtcc gagccgcacg ccgtacccga gcatcgactc ggcggggacg
     1081 acgacgcgca catgctcggg tgcgtcgtgc tgcggccaca acatcgcgac agtcatcgcg
     1141 aacacggagc ccgcgacgat gagcagagcg acgccagcac cctttccggc gttatagctg
     1201 aggccgacgc cgacgagcgg caccgacaac gacaaggcga tccggccgac cggccgcttc
     1261 gtcgacagct gcgccgcgcc gaccgcgagc acaaacacgc tcgcgacggc aagcaccggc
     1321 caggacgcca acgacgcgcc gacggcgagc ggaacgccca tcagcacacc catcaggacg
     1381 atgcgcaccc ggtcgcggcg gcgaggcgcg atcccgacga tcgccgccgg caacacgccg
     1441 aacccgagcg ccaggccctt gtcgaccgat accggcatgg ccagtgcagc cggaattgcg
     1501 aacgccgcgc cggccaacgc cgcgggccag gcccagacaa tccctggttg gcgcgccggc
     1561 ggcatcccga agatttatct catacgttgg gccgcagctg tcgatccggc ccgatcccgt
     1621 tcgtcgttgg agcaacacaa cacaccgatg gggaggaaca acccatgcgt tacgcactct
     1681 tgatctgcac cgacgaagcg gcatcggcgg cgtggtcgcc cgaagagcag gccgcgtcga
     1741 ccgccgcgta catggcgtgg ggtgaggaga tgggcaagcg cggactgctc accggcggcg
     1801 agcggctgcg accgatcacc gaggccacga ccgttcgcgt gcgcgacggc gaagtgctca
     1861 ccaccgacgg cccgttcgcg gagacgaagg aacaagtcgg cggcttttac ctcgtcgact
     1921 gcaaggacct cgacgaagcc atcgacattg cggcgaagat ccccggcgcc gccgtgggca
     1981 gcatcgaagt gaggccgatc tgggagatgt agaggccgcc gtcgccgccg cgtttcagtc
     2041 ggagtggggc cgtgtcgtcg ccacgctgat tcgaacaacc ggcgattggg acctcgccga
     2101 ggagtgcgcg caggatgcct tcgccaaggc cctcgaacga tggccgcgcg agggcgttcc
     2161 gcgcagtccc ggcgcgtggc tcacgacgac ggcgcgcaac cgcgcgttcg atgtacttcg
     2221 acgcgcggca gttggccgga acaagacgca ggaggcggcc gtgcttcccg gcgagccgga
     2281 tgcggacgat gacgacagcg gcgtgaccga cgatcgcttg cgcctcatct ttacctgctg
     2341 ccatcccgcg ctcggcctcg aagcgcaggt cgcgctcacg ttgcgcaccc tcgcgggcct
     2401 gaccaccgac gagatcgcgc gcgcattcct cgtgcccgag gcgacgatgg cgcagcgcct
     2461 ggtacgggcg aagaagaaga tccgcaacgc cggcatcccc tatcgcgtcc cgcccgcaca
     2521 tctcctgccc gagcggaccg tggcggtgct cggcgtgctc tatctgctgt tcaacgaggg
     2581 ctacgccgcg acgagcggcg agaacttgat ccgccagaac ctctcg
//
LOCUS       DASUGA010000002         4926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1248792, whole genome shotgun sequence.
ACCESSION   DASUGA010000002 DASUGA010000000
VERSION     DASUGA010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248792"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..450)
                     /locus_tag="VFX21_00030"
     CDS             complement(7..450)
                     /locus_tag="VFX21_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HEX5094358.1"
                     /translation="MIRGFRNFLMQGSVVTIAIGLMVALAFSTLIKAFTDAIVTPILN
                     RLAGDGPSDVGLGIQLGNEGNEKTFINIGDVLSAILYFLIFMGVLYFLIVVPYKAIMA
                     RRGEVVFGEPAPVKTCPACLSDDLPAAASKCRYCGTEQAASATST"
     gene            complement(552..1064)
                     /locus_tag="VFX21_00035"
     CDS             complement(552..1064)
                     /locus_tag="VFX21_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094359.1"
                     /translation="MASGDNGEHADNDDEHEAYGDALPDDLDVTAYVGPYEFPDIRRR
                     RIAGTIYAVIAAICVWAAASSGNEGFALAAVLLGLCAVYHFIAGWPLRVDQTEALATA
                     SRTVGFPVGHASAQLAWRGLRSRPTWRILVYSADEPPSIRGLVELDAVDGSVLGEYTE
                     DNPEDWAQYS"
     gene            complement(1119..1556)
                     /locus_tag="VFX21_00040"
     CDS             complement(1119..1556)
                     /locus_tag="VFX21_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094360.1"
                     /translation="MQAAELLTVRDAQVLLGRPVGRIEPLNDESAGARKPSDFICFGG
                     RTKGPTMVGEFVSLAAGPSANGARHVPFFSVALEGTRASAEDPCRRSVSARWEQAGLR
                     FSLTYTCIALADSDDVSALTNAKREPVAALARLVSDRVTSFLR"
     gene            complement(1609..2121)
                     /locus_tag="VFX21_00045"
     CDS             complement(1609..2121)
                     /locus_tag="VFX21_00045"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX5094361.1"
                     /translation="MSLDRLWADWRASYVADPATGTETHGCVMCNLVAAEDDAAALVL
                     ERTVHTITVMNLYPYGSGHLMVAPVRHVASYVDLSDDELVGLARAQVRALQAVHSAYA
                     PDGCNTGANIGRAAGAGVPNHLHVHVLPRWQGDTNFMTAIGEARVLPESLRAGYQKLQ
                     GVWPPVDGRV"
     gene            complement(2142..4148)
                     /gene="thrS"
                     /locus_tag="VFX21_00050"
     CDS             complement(2142..4148)
                     /gene="thrS"
                     /locus_tag="VFX21_00050"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX5094362.1"
                     /translation="MSEQITVTLPDGSGREYDRGTTPADVAASIGRGLAKAAIAAKAS
                     GDWIDLSTPLDHDTDLAIITPDTADGREVLRHSTAHVMAEAVTRLFPGAKYTIGPAIA
                     DGFYYDFDLPGGATFSADDLERIEAEMRAIVKNDQRFVRDELSYDDALALFADQPYKQ
                     EIIEKVRGGNADAEDAGEVGGDGGVSIYRNVVDGDVEFIDLCRGPHVPSTGKLGAFKL
                     TTVAGAYWRGNEKGPMLQRIYGTAWESQKALDEYLHRIEEAEKRDHRKLGVELDLFSF
                     PEVIGSGLAVFHPKGGIVRRVMEEYSRQQHEHAGYEFVNSPHLSKEDLFQISGHLDWY
                     ADGMYPPMHFDEGGQGGEGTNYYLKPMNCPFHILIYQSRTRSYRELPMRLFEFGTVYR
                     YEKSGVVHGLTRARGFTQDDAHIFTTRENMIEELESLLDFVLNLLRDYGLTDFYLELS
                     TKPEEKAVGSDEEWDEATEALRVAASSKDLELVLDEGGGAFYGPKISVQAKDAIGRTW
                     QMSTIQLDFQLPQRFDLHYVGADNERHRPIMIHRALFGSLERFFAVLVEHYAGAFPLW
                     LAPVQATVLPVADRHDAYATRLVDRLNAEGYRAEMVDGSAGALGHRIRNAKLQKVPYV
                     LVVGDDDVEHGTVGVNKRGTNDPERGVRVDDLVDRFAAEVIAHE"
     gene            complement(4256..>4926)
                     /locus_tag="VFX21_00055"
     CDS             complement(4256..>4926)
                     /locus_tag="VFX21_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094363.1"
                     /translation="TTANTAEVLDNEMEVDAIAASKILSESTVWATTTRVAPAPYENV
                     ERASNGVVVGTVVDIELGTPRTVDAATVFGDIDELPGATFEAVLAYLVVDVEDVVGGA
                     ELEALLPGETVAVPVAVWFSGAGAPQRDDVTADVVTRLRDAIPQGARLAVHAAGIDTA
                     GAVSSAPELLLPEMVGLPTPAAITFEASNGNLVNLDWLVADDAETYLGVTSFDELVER
                     AAQQ"
BASE COUNT          783 a         1658 c         1635 g          850 t
ORIGIN      
        1 tggcagctac gtgctggttg ccgacgcggc ctgttcggtg ccgcagtacc gacacttgga
       61 agcggcggcg ggaagatcgt cggagagaca tgccgggcac gtcttcaccg gtgccggctc
      121 accgaacaca acttcaccgc ggcgcgccat gatcgccttg tacggcacga cgatcaggaa
      181 gtacagcacg cccatgaaga tgaggaagta gaggatcgcg gagagcacgt ccccgatgtt
      241 gatgaacgtc ttctcgttgc cctcgttgcc gagctgaatt ccgagaccga cgtcgctcgg
      301 gccatcgccc gcaagtcggt tgaggatcgg agtgacgatc gcgtcggtga atgccttgat
      361 caacgtgctg aacgcgagcg cgaccatcag gccgatcgca atcgtgacca cgctgccttg
      421 catcaagaag ttcctgaatc ctctgatcac agctcatccc ttcaaggcct acgggaaccg
      481 ttgaggattg ttgacctttc acgcgtctcg caaacgcctg ctctcggacc cgagcgccag
      541 gccgtgaacg ctcagctgta ctgcgcccag tcctcggggt tgtcttcggt gtactcaccg
      601 aggaccgagc catcgaccgc gtcgagctcg acgagaccgc ggatgctcgg cggttcgtcg
      661 gcgctgtaga cgaggatgcg ccatgtcgga cggctgcgca ggccgcgcca cgcgagctgc
      721 gcggacgcgt gaccgaccgg gaacccgacg gtgcggctcg cggtcgcgag cgcttcggtc
      781 tggtcgacgc gcagcggcca tcccgcgatg aagtggtaca ccgcacaaag cccgagcagc
      841 accgccgcga gcgcgaaacc ctcgttcccg ctcgatgccg cggcccacac gcagatcgcg
      901 gcgatcaccg catagatcgt cccggcgatg cgccggcggc ggatgtcggg gaactcgtac
      961 ggcccgacgt acgcggtgac gtcgaggtca tccggcagcg cgtcgccgta tgcctcgtgc
     1021 tcgtcgtcgt tgtcggcgtg ttcgccgttg tcgccgctgg ccatcggccc gcgacggtag
     1081 ccccgggcgg gcccgttcct cccacccgct ccccgccctc agcgcaggaa cgaagtgacc
     1141 cggtcgctca ccaggcgggc aagggcggcg acgggctcgc gcttcgcgtt cgtgagggcg
     1201 ctcacgtcgt cgctgtcggc gagcgcgatg caggtgtacg tcagcgagaa gcggagtccg
     1261 gcttgttccc agcgcgccga cacgctgcgg cggcacgggt cctccgccga tgcgcgtgtc
     1321 ccttcgagcg cgaccgagaa gaacggcaca tggcgcgcgc cgttcgcgga cggacccgcg
     1381 gcgagtgaca cgaactcacc gaccatcgtc gggcccttgg tgcgcccgcc gaagcagatg
     1441 aagtcgctcg gcttgcgcgc gccggcactc tcgtcgttca acggttcgat ccgaccgacc
     1501 ggccggccca acaacacctg cgcgtcgcga acggtcagca gctcagccgc ttgcatcccg
     1561 cgctcccgtc catggcaacc acccccaccc cggcgccggc tggaaaggct agacgcgacc
     1621 gtccacaggc ggccagaccc cttgcagttt ttggtaaccg gcgcgcaggc tctcgggcag
     1681 cacgcgcgcc tcgccgatcg ccgtcatgaa gttcgtgtcg ccctgccagc gcggcagcac
     1741 gtggacgtgc aggtggttcg ggacgcccgc gccggccgcg cggccgatgt tcgccccggt
     1801 gttgcagccg tcaggcgcat aggcggaatg gactgcctgc agcgcgcgga cctgcgcgcg
     1861 ggccaggccg accagctcgt cgtcggagag gtcgacgtag cttgcgacgt gccggacggg
     1921 cgcgaccatc aagtgccccg acccgtacgg atacagattc atcacggtga tcgtgtgcac
     1981 ggtgcgctcg aggacgagcg cggcggcatc gtcttcggcg gcgacgaggt tgcacatcac
     2041 gcagccgtgc gtctcggtgc ccgtcgccgg atcggcgacg tacgacgcgc gccagtcggc
     2101 ccacagccgg tcgagcgaca tgtcctcgac gctactcgtg gctactcgtg ggcgatgact
     2161 tcggccgcga accggtcgac gaggtcgtcg acgcgcacgc cgcgttcggg gtcgttcgtg
     2221 ccgcgcttgt tcacacccac ggttccatgc tcgacgtcgt cgtcaccgac cacgagcaca
     2281 tacggcacct tctgcagctt ggcgttgcgg atgcggtggc cgagtgcgcc ggcgctgccg
     2341 tcgaccatct cggcgcggta gccctcggcg ttcaagcggt cgacgagtct cgttgcgtag
     2401 gcgtcgtgac gatcggcgac cggcaggacc gtcgcctgca ccggcgccag ccacaacggg
     2461 aacgcacccg cgtagtgctc gaccaatacc gcgaagaacc gttcgagcga accgaacaac
     2521 gcgcggtgga tcatgatcgg ccggtgccgt tcgttgtccg cgccgacgta gtggagatcg
     2581 aagcgttgcg gcagctggaa gtcgagctgg atcgtcgaca tctgccaggt acggccgatg
     2641 gcatccttcg cctgcaccga gatcttcggt ccgtagaacg cgccaccgcc ttcgtcgagc
     2701 acgagctcga gatccttgct cgacgcggcg acgcgcaacg cctcggtcgc ttcgtcccac
     2761 tcctcgtcgc tgccgaccgc cttctcctcc ggcttggtcg acagctccaa gtagaagtcg
     2821 gtgaggccgt agtcgcgcaa caggttgagg acgaagtcga gcaacgactc cagctcctcg
     2881 atcatgttct cgcgcgtggt gaagatgtgt gcgtcgtctt gcgtgaaccc gcgcgcccgt
     2941 gtgagcccgt gcacgacgcc ggacttctcg tagcggtaga cggtgccgaa ctcgaacagc
     3001 cgcatcggca gctcgcggta cgagcgcgtg cgcgattggt agatcaggat gtggaacggg
     3061 cagttcatcg gcttgaggta gtagttcgtg ccctcgccgc cctgaccgcc ttcgtcgaag
     3121 tgcatcggcg ggtacatgcc gtccgcgtac cagtcgaggt ggccggagat ctggaacaga
     3181 tcctccttcg acagatgcgg tgagttcacg aactcgtagc cggcatgctc gtgctgctgg
     3241 cgggagtact cctccatcac gcggcggacg atgccgccct tcgggtggaa caccgcgagt
     3301 cctgaaccga tgacctcggg gaacgagaac aagtcgagct cgacgccgag cttgcggtgg
     3361 tcgcgcttct cggcctcttc gatgcgatgg aggtactcgt cgagcgcctt ctgcgactcc
     3421 cacgcggtgc cgtagatgcg ctggagcatc gggcccttct cgttgccccg ccaatatgcg
     3481 ccggccaccg tcgtgagctt gaacgcgccg agcttgccgg tgctggggac gtgcggcccc
     3541 cggcacaggt cgatgaactc gacgtcgccg tcgacgacat tgcgatagat cgacacgccg
     3601 ccgtcgccgc cgacctcgcc cgcgtcctcg gcgtccgcgt tgccgccgcg caccttctcg
     3661 atgatctctt gcttgtacgg ctgatcggcg aacagcgcga gtgcatcgtc gtacgagagc
     3721 tcgtcccgca cgaagcgctg gtcgttcttc acgatggcgc gcatctcggc ttcgatgcgc
     3781 tcgaggtcgt cggcgctgaa cgtcgcgccg ccgggcaggt cgaagtcgta gtagaagccg
     3841 tccgcgatcg cggggccgat cgtgtacttc gcaccgggga acagccgggt gacggcctcg
     3901 gccatgacgt gcgcggtcga gtgccgcagc acctcgcgtc cgtcggcggt gtcgggcgtg
     3961 atgatcgcga gatcggtgtc gtggtcgagc ggcgtcgaga gatcgatcca gtccccgctc
     4021 gccttggccg cgatcgcggc cttggccagg cccctcccga tggaagcggc cacgtcggca
     4081 ggcgtcgtgc cacggtcata ctcccggccg gaaccgtcgg gaagcgtgac tgtgatctgc
     4141 tcgctcatct cacatctcgg tcggctggag gcgggtcggc cgagggtact cgctcgcggt
     4201 cgctcgaagg tgcgaaatcc tgtggcgacg ggccgcgctg gcttggtctt gattgctact
     4261 gctgagcggc cctttcaacg agctcatcga atgacgtcac gcccaggtag gtctctgcgt
     4321 cgtcggcgac gagccagtct aggttgacga ggttgccgtt cgaggcctcg aacgtaatgg
     4381 ccgccggtgt ggggagtccg accatctcgg gcagtagcag ttcaggcgct gaactcactg
     4441 cgccggccgt gtcgattcca gccgcatgca ctgcgagccg cgctccctga gggatcgcat
     4501 cacgcaatcg cgtgacaacg tcggcggtca cgtcgtcacg ttgaggcgcg ccggctccgc
     4561 tgaaccagac cgcgactgga acggcaaccg tctcaccggg caacaaggcc tccagctcag
     4621 cgccacccac aacatcctcg acatcgacga cgaggtaggc gagcaccgct tcgaacgtag
     4681 cgcccggcag ctcgtcgatg tctccgaaga cagttgctgc gtcgacggtg cgcggtgtcc
     4741 cgagttcgat atcgacaacg gtgcccacga cgacaccgtt gctcgcgcgc tcgacgttct
     4801 cgtacggcgc gggagcgaca cgcgtagttg tcgcccagac agtcgactcg gacaggatct
     4861 tcgacgcggc gatcgcgtcc acctccatct cgttgtcgag tacctctgcg gtgttggcgg
     4921 ttgttg
//
LOCUS       DASUGA010000003         2209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_135622, whole genome shotgun sequence.
ACCESSION   DASUGA010000003 DASUGA010000000
VERSION     DASUGA010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135622"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VFX21_00060"
     CDS             <1..341
                     /locus_tag="VFX21_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094364.1"
                     /translation="AVPVVFAIGVASILYSTSTTAIVQVESRPEMHGRVLALQSVLLV
                     GTTPIGGLLVGWLADTVGARAPLVLGGVVCLAAAAFGFVAARRHAGELYDLASTHGEL
                     PTDVLETTAA"
     gene            493..939
                     /gene="rplM"
                     /locus_tag="VFX21_00065"
     CDS             493..939
                     /gene="rplM"
                     /locus_tag="VFX21_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX5094365.1"
                     /translation="MRTYTPKAADVSRTWHVLDAEDQVLGRLATEAATLLRGKHKPTW
                     APHFDGGDHVIVINASALAVNPRKLADKRYYRHSGYPGGIKSESMEHLLARDPERVVR
                     LAVKGMLPKGPLGRQMLRRLRVYPGPNHPHVAQNPQPRVLSSARAR"
     gene            963..1355
                     /gene="rpsI"
                     /locus_tag="VFX21_00070"
     CDS             963..1355
                     /gene="rpsI"
                     /locus_tag="VFX21_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX5094366.1"
                     /translation="MPKPLIQTTGRRKEAVARVRLRPGTGQVVINGRAIESYFTIATH
                     RQAATEALRITQTADVYDVDASINGGGVSGQAGALRLGIARALVALDEEMRPSLKKAG
                     LLTRDAREKERRKYGLKKARKAPQYSKR"
     gene            1401..>2209
                     /locus_tag="VFX21_00075"
     CDS             1401..>2209
                     /locus_tag="VFX21_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX5094367.1"
                     /translation="MTLRFGTDGIRGVANRELTPELVTGVGRAAARVLGTARPFVVGR
                     DTRRSGPMLEAALVAGLCAEGADVLLAGVLPSPAVAELAHAREAPAAMITASHNAYAD
                     NGVKLFAVGGRKLSDEIERHVEHELRDLATTMPDGPEGRGVGVASELRGALDEYVAHV
                     VAALDDRRLGGLRVVVDCANGAAFRAAPRVLRELGAHVEVLHAAPNGSNINDGCGSTD
                     PSDLQRAVVAVGADAGLAFDGDADRLIAVDERGEIVDGDQLIAIAAFDLQER"
BASE COUNT          316 a          810 c          769 g          314 t
ORIGIN      
        1 tggcggtgcc ggtcgtgttc gcgatcgggg tcgcgagcat cctctactcc acctcgacga
       61 ccgcgatcgt ccaggtggag tcgcgaccgg agatgcacgg ccgggtcctc gcgctccaga
      121 gcgtgttgct ggtcgggacg acgccgatcg gcggcttgtt ggtgggctgg ctcgccgaca
      181 ccgtcggcgc ccgggccccg ctcgtgctcg gcggggtggt gtgtctcgcg gcggcggcct
      241 tcggcttcgt ggccgcacgg cgccacgccg gcgagctcta cgatctcgcc tcgacccacg
      301 gcgagctccc cacggacgtc ctcgagacca cggcggcctg accggcgcgg ccggcgattc
      361 ggctgccgag accgccagcc gacgatttcc gggttttcgg ccgagcaagt agtctctcgg
      421 gtctgcccgc ggcctcgtcc ggcccggcgc cccttcgccg aaatttggcg tttcgattct
      481 cgggattctg gaatgcgcac gtacacgccc aaggccgccg acgtcagccg cacgtggcac
      541 gtcctcgacg ccgaggacca ggtgctgggt cggctcgcca ccgaagcggc aacgttgttg
      601 cgtggcaagc acaagcccac gtgggcgccc cacttcgacg gcggcgacca cgtcatcgtc
      661 atcaacgcgt cggcgctcgc ggtgaacccg cgcaagctcg ccgacaagcg gtactaccgc
      721 cactcggggt acccgggcgg catcaagtcc gagagcatgg agcacctgct cgcgcgtgac
      781 cccgagcgcg tcgtgcgcct ggcggtcaag ggcatgctcc ccaagggccc gctcggtcgc
      841 cagatgctca ggcgcctgcg cgtgtacccc ggcccgaacc atccccacgt cgcccagaac
      901 ccgcagccgc gcgtgttgtc gtcggctcgg gcccgctgac gcgcaccccg agaggagttc
      961 cgttgcccaa gccgctcatc cagaccacgg gacggcgcaa ggaagccgtc gcgcgcgtcc
     1021 ggctgcgccc gggcacggga caggtcgtga tcaacggacg cgcgatcgag agctacttca
     1081 cgatcgccac gcaccgccag gccgccaccg aagcactgcg catcacccag accgccgacg
     1141 tctacgacgt cgacgccagc atcaacggtg gtggcgtgag cggtcaggcc ggcgcactgc
     1201 ggctcggcat cgcgcgtgcg ctcgtcgcgc tcgacgaaga gatgcgcccg tcgctcaaga
     1261 aggccgggct gctcacgcgc gacgcgcgtg agaaggagcg ccgcaagtac ggtctgaaga
     1321 aggcgcgcaa ggctccgcag tactcgaagc ggtgaccgtc gcgcgtcgtc gcggcccttc
     1381 gaacgaatca tgacagctcg atgacgctgc gtttcggcac tgacggcatt cgcggtgtcg
     1441 ccaatcgcga gctgacgccg gagctcgtca ccggcgtcgg acgcgcggcc gcacgcgtgc
     1501 tcggcaccgc gcgtccgttc gtggtcgggc gcgacacccg ccggtcggga ccgatgctcg
     1561 aggccgcact ggtcgccggg ctctgcgccg aaggcgccga cgtgctgctc gccggggtgc
     1621 tgccgtcacc ggcggtggcc gagctcgcgc acgcgcgtga agcgccggcc gcgatgatca
     1681 ccgcgagcca caacgcatac gccgacaacg gcgtgaagct gttcgcagtt ggtggccgca
     1741 agctctccga cgagatcgag cgtcacgtcg agcacgagct gcgcgatctc gccaccacca
     1801 tgccggacgg acccgagggt cgcggcgtcg gagtcgcgtc ggagctccgt ggcgccctcg
     1861 acgagtacgt ggcgcacgtc gtcgccgcgc tcgacgaccg ccggctcggc ggcctgcgcg
     1921 tcgtcgtcga ctgcgcgaac ggcgcggcgt tccgcgccgc gccgcgcgtg ttgcgcgagc
     1981 tcggcgcgca cgtcgaggtg ctccacgcgg cacccaacgg ctcgaacatc aacgacggct
     2041 gcggatccac cgacccgtcc gacctgcagc gggctgtggt cgcggtcggc gccgacgccg
     2101 gtctcgcgtt cgacggcgac gccgatcgtc tcatcgcggt cgacgagcgc ggtgagatcg
     2161 tcgacggcga ccagctgatc gccatcgccg ccttcgacct ccaggagcg
//
LOCUS       DASUGA010000004         4409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_414496, whole genome shotgun sequence.
ACCESSION   DASUGA010000004 DASUGA010000000
VERSION     DASUGA010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414496"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="VFX21_00080"
     CDS             complement(<1..1244)
                     /locus_tag="VFX21_00080"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5094368.1"
                     /translation="MTLRIRLAGPLEVCAGATRLGPGDFPGRQGRLVFAALVRARRPV
                     DRDELAEMLWPNELPASWTRDLSAIVSKLRALLRGVATFSTGGGRWYAIDLPADVTVD
                     IEDAIALVEQAEKREGDAAAAAVRGALLLAEPFLPGDECAWVDECRTELRALLARALV
                     VQAECLIDAADAGAVRPADALVALEPDQERSHLLLVRAHLAANDRVGALRAYDALRRR
                     MADTFGLSPSAEAESLLRVALGSETEMPIAIIEATRGPVVGRERDLLKLRLLLDPSSP
                     VRVALIVGEPGIGKSRLAAEIAAEAHSAGFEVELGDAESVAPDRRVLVVVEDVHALDR
                     DQVARLQRLVDHAAGVRIVATARTPDVEHAPVQSWIADLRARDVVMTVALTGLDERDV
                     AAMLGGRAEFAAMVRRSTGGNPL"
     gene            1331..2392
                     /locus_tag="VFX21_00085"
     CDS             1331..2392
                     /locus_tag="VFX21_00085"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5094369.1"
                     /translation="MTAIDEAKLDAFMGQAIGDLGAAISGLMVYLGDQLGLYRAMAGA
                     GPVSSADLAKATGTDERYVREWLNNQAAGGYVTYDPKAQNYTLPPEQAMALADEDSPA
                     FLPGGYSGIVSAYIDADKFVDAFRTGRGVGWHEHDARLFHGTERFFRPGYKSFLVADW
                     IPALELAPAKLAAGATVADIGCGHGASTVLMAQAFPKATFVGYDYHDASIETARKRAA
                     DAGVSDRVTFEVASAKDYPQRKYDLITFFDCLHDMGDPVGAVVHARRALADDGTVMLV
                     EPNAGDAPEDNHHPLGRAYYGLSTVICTMASRAQEVGLALGAQAGEKPMREIFEQAGF
                     GTFRRATETPTNIVYEARP"
     gene            complement(2429..2836)
                     /locus_tag="VFX21_00090"
     CDS             complement(2429..2836)
                     /locus_tag="VFX21_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5094370.1"
                     /translation="MSNRGLDILTAEESNELLTTHTFGRVVAKVGAEPAAYPVYYAFV
                     DGTIVYRTDPGTKLAAAVLHNRVSFEVDSDVEGWSVMAFGPCEEVRRPHEIDACHAAL
                     GTEWPDAERQRVVRIRPDRITGRRLQRPRSAEE"
     gene            complement(2904..3308)
                     /locus_tag="VFX21_00095"
     CDS             complement(2904..3308)
                     /locus_tag="VFX21_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEX5094371.1"
                     /translation="MEDDEMRARVGAARVGRLATVTAAGRPHVVPCCFVLAGDVVYTA
                     VDAKPKSTLALRRLDNIAAHPHASLLVDFYDDDWRQLWWVRVDGTARVLTGGAERDDA
                     LTRLAEKYRQYAETPPPGPMIAIAVSAWRAWP"
     gene            complement(3325..>4409)
                     /locus_tag="VFX21_00100"
     CDS             complement(3325..>4409)
                     /locus_tag="VFX21_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5094372.1"
                     /translation="AILFIAACAALILPEVIDDDGAAEYDVALVAPTDAESSFRSTLD
                     GVLEQLDASARFRTVASVDDARRLVDDGDADVGVAAGPEPVIVVRADQHAKLLGAVRQ
                     ALASSALADNLANAGLSDAQVVQVLRVQAPRVQEVDTEAAGRRASAAILSIVLYLVLL
                     MLMVQVANGVAIEKANRVSEVLLPIVRPDSLLFGKVIGTGIVGLITLLAGAIPVAVKA
                     VAGGDLPSGLGVAVAGGAPWFVLGLVLYLTIAGSLGALVERQEEAGATVTPLTIILVA
                     TFIVAQSAPDSGLAAVLAYIPFTSPLVMPSRIAVGASSPLEMTLSLLILFTTVVVVVR
                     LGAVVYQRAVVRTGRRLKITEILRAA"
BASE COUNT          735 a         1589 c         1483 g          602 t
ORIGIN      
        1 agcgggttcc cgccggttga cctgcgcacc attgccgcga actcggcgcg accgccgagc
       61 atcgccgcaa catcgcgttc gtccagtccc gtcaacgcga ccgtcatgac gacatcacgg
      121 gcgcggagat cagcgatcca cgactgcacc ggggcgtgct cgacgtcggg agtgcgcgcc
      181 gtcgcaacga tgcgtacgcc cgcagcgtgg tcgacgagcc gttgcaggcg cgcgacctga
      241 tcacgatcga gcgcatgcac gtcctccacg accacgagca cacggcgatc cggggccacc
      301 gactcggcat caccgagctc cacctcgaag cccgcggaat gcgcttccgc cgcgatctcg
      361 gccgccaacc ggctcttgcc gatcccgggc tcacccacga tcagcgcaac gcgcaccggc
      421 gacgacggat cgagcaataa ccgcagcttc aagagatcgc gctcgcgtcc gacgaccggg
      481 ccacgcgtcg cctcgatgat tgcgatgggc atttcggtct cggaaccgag cgcgacgcgc
      541 aggagcgact ctgcttcggc cgacggtgac agcccgaacg tgtccgccat gcgccgtcgc
      601 agggcgtcgt acgcgcgcaa cgcgccgacc cggtcgttcg cggccaagtg cgcacgcacg
      661 agaaggaggt gtgagcgttc ctggtccggt tcgagcgcaa ccaacgcgtc cgccgggcgt
      721 acggcacccg cgtcggccgc gtcgatcagg cactccgcct gcacgacgag cgcgcgggcc
      781 aacaacgcgc gcagctcggt gcggcactcg tccacccacg cgcactcgtc acccggcagg
      841 aacggttcgg cgagcagcag cgccccgcga actgccgcgg ccgccgcgtc cccctcgcgc
      901 ttctcggcct gctcgaccag ggcgatcgcg tcctcgatgt cgacagtcac gtcggcgggc
      961 aggtcgatcg cgtaccaccg gccgccaccc gtgctgaacg tcgcaacgcc gcggagcagc
     1021 gcgcgcaact tcgagacgat cgcggacagg tcgcgggtcc acgacgcggg cagctcgttg
     1081 ggccagagca tctccgccag ttcgtcgcgg tcgaccggcc ggcgggcgcg gaccaaggcc
     1141 gcgaacacga gacgtccctg gcggccgggg aagtcgccgg gcccgaggcg cgtggcaccg
     1201 gcgcatacct cgagcggacc ggcgagacgg atccgcagcg tcacgttcct gcacaccaaa
     1261 ccgacgacaa accggcctcc gtaccttcat ccatcgaagc agtccggtgt agcccgttca
     1321 aggaggggca atgacagcga tcgacgaagc gaagctcgac gccttcatgg gtcaggcgat
     1381 cggcgacctg ggcgccgcca tctccggcct gatggtgtac ctcggcgacc agctcgggct
     1441 ctatcgagcc atggccggcg ccgggcccgt gtcgtccgcc gatctcgcga aggccactgg
     1501 gaccgacgag cgctacgtgc gcgagtggct caacaaccaa gcggccggcg gttacgtcac
     1561 ctacgacccg aaggcgcaga actacacgct gccgcccgag caggcgatgg cgctcgccga
     1621 cgaagacagc ccggcgttcc tccccggcgg ttactcgggc atcgtctccg cgtacatcga
     1681 cgccgacaag ttcgtcgacg ccttccgcac cgggcgcggc gtcggctggc acgagcacga
     1741 cgcgcgcttg ttccacggca cggagcgctt cttccggccg ggctacaagt cgttcctcgt
     1801 tgcggactgg attcccgcgc tcgaactcgc gcccgcgaag ctcgcggccg gtgcgaccgt
     1861 cgccgacatc ggctgcgggc acggcgcgtc gacggtgttg atggcgcagg cgttcccgaa
     1921 ggcgacgttc gtcgggtacg actaccacga cgcgtcgatc gagaccgccc gcaagcgggc
     1981 cgcggacgcc ggtgtctccg accgcgtgac gttcgaagtc gcgtcggcga aggactaccc
     2041 gcagaggaag tacgacctca tcaccttctt cgactgcctg cacgacatgg gcgacccggt
     2101 cggtgcggtc gtgcacgcgc gccgggccct cgcggacgac ggcaccgtga tgctggtcga
     2161 gccgaacgcg ggggacgcgc ccgaggacaa ccatcacccg ttggggcgcg cgtactacgg
     2221 cctgtcgacg gtcatctgca cgatggcgtc gagggcgcag gaagtcggtc tcgcgttggg
     2281 tgcgcaagcg ggcgagaagc cgatgcgcga gatcttcgag caggccgggt tcggcacgtt
     2341 ccggcgcgcg acggaaacgc cgaccaacat cgtctacgaa gcgcgtccgt agccgccggt
     2401 cgcaagcgga ggcgtatcgg cgcggccgct actcctccgc cgaccggggg cgctgcaaac
     2461 ggcggccggt gatccggtcg ggccggatgc gcacgacgcg ttggcgctcg gcatcgggcc
     2521 attcggtgcc gagtgccgcg tggcacgcgt cgatctcgtg cggtcgccgg acctcctcgc
     2581 agggcccgaa cgccatgacg ctccatccct cgacgtcgct gtcgacctcg aaggacaccc
     2641 ggttgtgcaa gacggccgcc gccagcttcg taccgggatc ggtgcggtag acgatcgtgc
     2701 cgtcgacgaa cgcgtagtac acgggatacg ccgcgggctc ggcgccgact ttcgccacga
     2761 cgcgaccgaa cgtatgcgtc gtcaacagct cgttgctctc ctcggcggtc aagatgtcga
     2821 ggccgcgatt gctcatatcg ccaccgtcgc gctcgcggcc ttccgccact agaggccaaa
     2881 gtcccgcgtc gacggccgcc gggtcagggc caggcgcgcc acgccgacac ggcgatcgcg
     2941 atcatcggcc cgggcggagg cgtctcggcg tactgccggt acttctccgc cagccgcgtg
     3001 agcgcgtcgt cgcgttcggc accgccggtg agcacgcgcg cggtgccgtc gactcgcacc
     3061 caccacaact ggcgccagtc gtcgtcgtag aagtcgacga gcaacgacgc atgaggatgc
     3121 gcggcgatgt tgtcaaggcg ccgcagggcg agggtcgact tcggcttggc gtcgaccgcc
     3181 gtgtagacga catcaccggc gagcacgaaa cagcacggca ccacgtgcgg acggcccgcg
     3241 gcggtcacgg tcgccaggcg cccgacccgg gcggcgccga cccgagcgcg catctcgtcg
     3301 tcctccatcg ccgatgacgg tacgtcacgc ggctcgcagg atctcagtga tcttcagtcg
     3361 acggccggtg cgcacgactg cgcgctgata gacgacggca cccagccgca ccacgacgac
     3421 aaccgttgtg aacaggatca gcagcgacag cgtcatctcg agcggcgacg acgctccgac
     3481 ggcgatccgc gacggcatca ccaacggtga cgtgaacggt atgtaggcga gcacggccgc
     3541 gagacccgaa tccggcgcgc tctgggccac gatgaacgtc gcgacgagga tgatcgtcag
     3601 tggagtcacg gtcgcgcccg cctcctcctg gcgttccacc agtgcgccga gcgaaccggc
     3661 gatcgtgagg tacaggacga gcccgagcac gaaccacggc gcgccgccgg caacggcgac
     3721 gcccaaaccc gagggcaggt caccgcccgc gaccgccttc accgcgaccg ggatcgcgcc
     3781 ggccaggagc gtgatcaggc cgacgatgcc ggtaccgatc accttcccga acagcagcga
     3841 gtcgggccgc acgatcggca gcagcacctc actcacgcgg ttggccttct cgatcgcgac
     3901 gccgttcgcg acctgcacca tcagcatcaa cagcacgagg tagaggacga tcgacaagat
     3961 cgcggccgac gcccgccggc ccgcggcttc ggtatctact tcctgtacgc gtggcgcttg
     4021 gacgcgcagc acctgcacga cctgcgcgtc gctcagaccg gcgttcgcga gattgtcggc
     4081 cagcgcgctc gatgcgagcg cttgccggac tgcgccgagg agcttggcgt gttgatccgc
     4141 gcgtacgaca atgaccggct ccggtcccgc cgcgacaccg acgtcggcgt cgccgtcgtc
     4201 gaccagccgg cgtgcgtcgt cgacgctcgc gacggtccgg aatcgggcac tcgcgtcgag
     4261 ttgctccagc acgccatcga gtgtcgatcg gaacgagctc tccgcgtcgg tcggggcgac
     4321 gagggcaacg tcgtactcag ccgcgccgtc gtcgtcgatg acttcaggca ggatcagcgc
     4381 agcacacgcg gcgatgaaca agatcgcca
//
LOCUS       DASUGA010000005         2382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1247601, whole genome shotgun sequence.
ACCESSION   DASUGA010000005 DASUGA010000000
VERSION     DASUGA010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247601"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..1405)
                     /locus_tag="VFX21_00105"
     CDS             complement(122..1405)
                     /locus_tag="VFX21_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5094373.1"
                     /translation="MTAIETTDGAAEDVEQFRQRARDWIRTNLRTMGSDDLAGFMRGM
                     SDERELAAVAHERELQHKLFDAGLAGICVPVEYGGQGLTPAHQAALNEELKGYEYPVR
                     IQSPTMSPCLAVLLDFGTEEQKREHVPAILRGDEIWGQFLSEPSGGSDVAGALTTAVR
                     DGDEWVLNGSKIWTSGAWWTDWALCLARTNWDVPKHRGLTVFMFAIHQPGIELHRIEM
                     LNGNREFCQEFMTDLRVPDTQRVGEVDQGWTVGTRWMFHERMAMAHNSPLLTEPPSGR
                     RRLADARALVGVASELGRIDDTYVRDLIGEAHMLELVSTHLQPRLGAAMAYGAMSEQA
                     ASIGRLFGGVVAGHTATIAFEVAGAAGGAWAESDGAVVGAGTGFLVRQIAAIGGGTLE
                     MARNVIAERVLGMPREQSFDRDVPFRDVPRGPAHG"
     gene            complement(1402..>2382)
                     /locus_tag="VFX21_00110"
     CDS             complement(1402..>2382)
                     /locus_tag="VFX21_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019930438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX5094374.1"
                     /translation="TSLLVGDAHGGGSISGVGLVDLSVIAHEFGAHAAPGPLVTVNVV
                     ADALTRAGDAHADVLGDLLAGTVIGSWCSAEPPPLDRLGAIGLEVRVDGDDVVLDGVK
                     RPVESAAQAGVLLVTGRTEDGLTQVLVPADTPGVSIVPMDTVDLTRRFGVVTFDGVRV
                     PLDAVVGEVGGAAAQVERQYQIAAALSSAESVGAMQTSFDMTVEWAFERYSFGRPLAS
                     YQEIKHRFADMKSWLEASHGITDAAIDAIAADASDAAELASAAKAYTGQYGAELVQDC
                     VQMHGGIGVTFDHDLHLYLRRVTLNRALYGTPADHRQRLAALVEQQAVHA"
BASE COUNT          364 a          853 c          807 g          358 t
ORIGIN      
        1 catcatcagc gggtacggcg agtgatcgcg cacgagcgcg cgcacggcgt cgcggtcgcg
       61 atacgcaggt tccacccaga ccggctcagc gatctcggcc acggtcatgg actgcagtct
      121 gtcagccgtg agcgggtccg cgcggcacgt cgcggaacgg gacgtcgcgg tcgaaggact
      181 gctcgcgcgg catcccgagc acacgctcgg cgatcacgtt gcgcgccatc tcgagcgtgc
      241 ctccgccgat cgccgcgatc tgacggacga ggaaccccgt gccggcgccg acgaccgcgc
      301 cgtcggattc ggcccatgcg ccgccggccg cgcccgccac ctcgaacgcg atcgtcgccg
      361 tgtgaccggc gaccacaccg ccgaagagtc gaccgatcga cgcggcctgc tcggacatcg
      421 cgccgtaggc catcgccgcg ccgagccgcg gctggaggtg cgtcgagacg agctcgagca
      481 tgtgcgcttc gccgatcaag tcgcgcacgt acgtgtcgtc gatgcgtccg agctcgctcg
      541 caacgcccac gagcgcacgc gcgtcggcga gtcgccggcg gccgctcggc ggctcggtga
      601 gcagcggaga gttgtgcgcc atcgccatgc gctcgtggaa catccatcgg gtgccgacgg
      661 tccagccctg atcgacctcg ccgacgcgtt gcgtgtcggg cacgcgcagg tcggtcatga
      721 actcctggca gaactcgcgg ttgccgttga gcatctcgat gcggtgcagc tcgatgccag
      781 gttggtgaat ggcgaacatg aacaccgtca acccgcggtg cttcggcaca tcccagttcg
      841 tacgggccag gcagagcgcc cagtcggtcc accacgcgcc gctggtccag atcttcgaac
      901 cgttgagcac ccactcgtcg ccgtcacgaa ccgcggtcgt gagcgcgccg gcaacgtcgg
      961 aaccaccact cggctcggac aggaactgtc cccagatctc gtcacccctg aggatcgcgg
     1021 gcacgtgctc acgcttctgc tcctccgtgc cgaagtcgag cagcaccgcc aggcatggcg
     1081 acatcgtcgg tgactggatc cgcaccgggt actcgtagcc cttcagctcc tcgttcagcg
     1141 cggcctggtg cgccggagtg agcccctggc cgccgtactc gacgggaacg cagatcccgg
     1201 cgaggccggc gtcgaagagc ttgtgctgca gctcccgctc gtgcgcgacg gcggcgagct
     1261 cccgctcgtc ggacatcccg cgcatgaacc cggcgaggtc gtcggagccc atggtgcgca
     1321 ggttggtgcg gatccagtcg cgcgcccgct gacggaactg ctcgacgtcc tccgccgcgc
     1381 cgtcggtggt ctcgatcgcc gtcatgcgtg caccgcctgc tgttcgacga gcgcggccag
     1441 acgttgacga tggtcggccg gggttccgta cagcgcacgg ttcagcgtca cgcgccggag
     1501 atagagatgg aggtcgtggt cgaacgtgac cccgatgccg ccgtgcatct gcacgcagtc
     1561 ctggacgagc tcggcaccgt actgaccggt gtatgccttc gctgcgctcg cgagctcggc
     1621 ggcgtcgctc gcgtcggccg cgatcgcatc gatcgcggca tcggtgatgc cgtgactcgc
     1681 ttcgagccac gacttcatgt cggcgaaccg gtgcttgatc tcctggtacg acgcgagcgg
     1741 ccgaccgaac gagtatcgct cgaacgccca ctcgaccgtc atgtcgaacg aggtctgcat
     1801 cgcgcccacc gactccgcgc tcgagagcgc ggctgcgatc tgatactgcc gttccacctg
     1861 tgctgcggcg ccaccgacct cgccgaccac ggcgtcgagt ggaacgcgta cgccgtcgaa
     1921 cgtgacgacg ccgaaccgcc gcgtgaggtc gaccgtgtcc atggggacga tcgacacgcc
     1981 cggggtgtcc gcagggacga gcacctgcgt gagcccatcc tcggtgcgac cggtcacgag
     2041 cagcacgccg gcctgggccg cggactcgac cgggcgcttg acgccgtcga gcacgacgtc
     2101 gtcaccgtcg acgcggacct cgagtccgat cgcgcccaat cgatcgagcg gcgggggctc
     2161 ggcagagcac cacgagccga tcacggtccc cgccagcagg tcgccgagca cgtcggcgtg
     2221 cgcgtcaccg gcgcgcgtga gcgcgtcggc gacgacgttc accgtgacga gtggtccggg
     2281 tgcggcgtgc gccccgaact cgtgggcgat gacactgagg tcgacgagcc cgacaccgct
     2341 gatgctcccg ccaccgtgcg cgtcgccgac gagcaacgac gt
//
LOCUS       DASUGA010000006         2443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_134647, whole genome shotgun sequence.
ACCESSION   DASUGA010000006 DASUGA010000000
VERSION     DASUGA010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134647"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..737)
                     /gene="ispF"
                     /locus_tag="VFX21_00115"
     CDS             complement(240..737)
                     /gene="ispF"
                     /locus_tag="VFX21_00115"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX5094375.1"
                     /translation="MRYRVGLGFDVHPFGDAPPLVLGGVRIPDAPKLTGHSDGDAVAH
                     AVADAVLGIAGLPDLGTLFPASDDSYRNADSIELLRDVAGRVAKAGWWIDNVDVAIAA
                     ETPRLGPHTSAMAERITDALHAARQPMGHGISVAVKPKRAEGLGAIGRAEGIAVWAIA
                     LLARG"
     gene            complement(734..1375)
                     /gene="ispD"
                     /locus_tag="VFX21_00120"
     CDS             complement(734..1375)
                     /gene="ispD"
                     /locus_tag="VFX21_00120"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX5094376.1"
                     /translation="MSVWAIVVAAGSGARFGAAKQFARLGGITVLDRAVGVASECSDG
                     VVVVLGPDADWSPPAGVDVVAGGATRSASVRNGLERVPADADVVVVHDAARPLASRGL
                     FVAVVEAVRAGADAAIPALPVVDTVKRVDGAHVIETVPREDLVVVQTPQAFRADALRK
                     AHAAADVSTDDAALVEAAGGTVVVVPGEPRNLKLTVTADLELAHALLDSGGAP"
     gene            complement(1516..>2443)
                     /locus_tag="VFX21_00125"
     CDS             complement(1516..>2443)
                     /locus_tag="VFX21_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094377.1"
                     /translation="CLLGYVTGGVFGRLLDRALGAVERHVDAVPPAQVVAGTLGAIAG
                     ASVAVVLVLPLALLLPTEAAVLLAGLVSWVAGFLGLRVLGRKSVAVLEMLGLSTRPLV
                     RAQPFDARDGLLVDSSVVMDGQLLPLMRAGILGSDLLVPRFVLDEVQGFADSVDDQKR
                     RRAQRGLEALEHLQREGPVRMFVLDDELPELADVDAKLVALARRLQLRLLTNDGPLAR
                     NAELQGVPTCNLRKLVQDISPTIVPGDFVRVALTRAGREVGQGVGHLDDGSMVVVNGG
                     SELVGGPDVQLQVTSVVPTSAGRMVFAKLDAS"
BASE COUNT          389 a          871 c          890 g          293 t
ORIGIN      
        1 ttcgcgagag aagtcctcga agcgcggcgt cgcagcccga aggctgtcgc cgcggtcgcc
       61 cgctgcttgc atgttcgccc gtcctcacaa ggtctcgggg ggagaccttt cgcccgtgtg
      121 agatgttcgc ccgttgccga ccgcctccca gcggccgaac ggtgccattc tcacgcagcg
      181 tggcaggtcg cgccaatcgg tcgaacggcc tataacgcga cacctcgcag tgacgtcggt
      241 cacccccggg cgagcagcgc gatcgcccag actgcgatcc cttcggcgcg gccgatggcg
      301 ccgaggccct ccgcccgctt gggtttcacc gcgacgctga tgccgtggcc catcggctgg
      361 cgggccgcgt gcagagcgtc ggtgatgcgc tccgccatcg ccgaggtgtg cgggccgagc
      421 cggggcgtct cggccgcgat cgcgacgtcg acgttgtcga tccaccaacc ggccttggcg
      481 acccggcccg cgacgtcgcg cagcagttcg atcgagtcgg cgttgcgata cgagtcgtcg
      541 gacgcgggga acaaggtgcc gaggtcgggc aggccggcaa tgccgagcac ggcgtcggcg
      601 accgcgtgcg cgacggcgtc gccgtcgctg tggcccgtga gcttcggcgc gtcgggaatg
      661 cgcacgccgc cgagcacgag gggcggcgcg tcgccgaacg gatggacgtc gaagccgagc
      721 ccgacgcggt acctcacggt gcgccgccgc tgtcgaggag cgcgtgcgcg agctccaggt
      781 cggcggtcac ggtgagcttc agattgcgcg gttcgccggg gacgacgacc acggttcctc
      841 ccgccgcttc gacgagcgcg gcgtcgtcgg tcgacacgtc ggccgccgcg tgcgccttgc
      901 gcaatgcgtc ggcccggaac gcctgcggtg tctgcacaac cacgagatcc tcacgcggga
      961 ccgtctcgat cacgtgcgcg ccgtcgactc gtttgacggt gtcgacgacg ggcaacgcgg
     1021 ggatggcggc gtcggcgccg gcgcgcaccg cttcgacgac ggcgacgaac aggccgcgcg
     1081 acgcgagggg acgggccgcg tcgtgcacga cgacgacgtc cgcgtcggcc gggacgcgtt
     1141 cgaggccgtt gcgtaccgag gccgagcgcg tcgcaccacc ggcgacgacg tcgacaccgg
     1201 cgggcggcga ccagtccgcg tccggtccaa gtacgacgac cacaccgtca ctgcactccg
     1261 acgcgacgcc caccgcgcgg tcgagcacgg tgatgccacc gagacgcgcg aactgcttcg
     1321 cggcgccgaa gcgcgcaccg ctgcccgcgg cgacgacgat ggcccagacc gacacgacgc
     1381 ggagactagg cacgaaaccg tcacaccgcg cggtgccgga gcttgcggag gcactcgcgg
     1441 agcggagggc gggccaacaa tccgggccgc ctcacgcgag cacgaccgga gcgacaaggg
     1501 gttacgggcg ggaagtcagg aggcgtcgag tttggcgaac accatgcggc cggccgacgt
     1561 cggcacgacc gacgtgacct ggagctgcac atcggggccg ccgacgagct ccgagccacc
     1621 gttcacgacg accatcgagc cgtcgtcgag gtgaccgaca ccctggccga cctcgcgtcc
     1681 ggcgcgggtg agcgcgacgc ggacgaagtc gccgggcacg atcgtcggcg agatgtcctg
     1741 caccagcttg cggagattgc acgtggggac gccctgcagc tccgcgttgc gcgccaacgg
     1801 accgtcgttg gtgagcaagc gaagctggag gcggcgcgcc agcgcgacga gcttggcatc
     1861 gacatcggcg agctcgggca gttcgtcgtc gaggacgaac atccgcaccg gcccctcgcg
     1921 ctgcagatgc tcgagtgcct cgagcccgcg ctgcgcgcgg cgccgcttct ggtcgtcgac
     1981 cgagtcggcg aagccctgca cttcgtcgag cacgaagcgc ggaacaagga ggtcggaacc
     2041 gaggatgccg gctcgcatca acggcaacag ctgaccgtcc atgaccaccg agctgtcgac
     2101 gagcagcccg tcacgcgcgt cgaacggctg cgcgcgcacg agtggacggg tcgacaaccc
     2161 gagcatctcg agcacggcga cgctcttgcg gccgagcacc cgcaagccga ggaatcccgc
     2221 cacccaggac acgagccccg cgagcagcac cgcagcttcg gtcgggagca gcaacgcgag
     2281 gggcaggacg agcaccaccg ccaccgacgc gcccgcgatc gcgccgagcg tgcccgcgac
     2341 gacctgcgcg ggcgggacgg cgtcgacgtg gcgctccacc gcgccgaggg cgcggtcgag
     2401 gagccggccg aacacgccgc cggtgacgta gccgagcagg cag
//
LOCUS       DASUGA010000007         3168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1941200, whole genome shotgun sequence.
ACCESSION   DASUGA010000007 DASUGA010000000
VERSION     DASUGA010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1941200"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VFX21_00130"
     CDS             <1..915
                     /locus_tag="VFX21_00130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5094378.1"
                     /translation="SRCYTTESGRRAVLPLASIGSGATTAYASPRRGWGYGGLIAEGG
                     VTPEDIAVASADMSRLRALKFHVRPNPLQAPMWEKFAPEMCRTRKEVHILDLRGGEDA
                     AFEKLHKSAKKGIKAAERCGVRIESASGDQLLGIFFELTYAARAQWAGQQHEPVWLAQ
                     LRGRFRDSERKWKNIAKHFGPNLRVYVAWCDDRPAAAGIVLRGRNVHGARAAMDREVA
                     NRSGATHALNWAVIKDACASGAQSFHMGESATKGAADFKRFLGAECVPFDEIDYERFP
                     LSRANRATRSLVKKVVGFKEDDVNAVVS"
     gene            complement(952..2661)
                     /locus_tag="VFX21_00135"
     CDS             complement(952..2661)
                     /locus_tag="VFX21_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5094379.1"
                     /translation="MHDLVIKGGTVVDGTGDAPRTADVAITDGVITEVGRVDGAAREM
                     VDADGLVVTPGFVDVHTHFDGQITWDPKLTPTCWHGVTTVVMGNCGVGFAPVHPDRHE
                     WLIGLMEGVEDIPGAALSEGIEWEWESFGEYLDAIERKPKMIDVGTQVPHGAVRGYVM
                     GERGAKNEPATPDDIDEMARIVRDGIAAGALGFSTSRTIAHMAIDGEPVPGTFAAEDE
                     LFGIGRVLGELGMGVFELAPAGALGEDLAAPDREMAWMRKLSAEIGRPVTFALTQNDH
                     DPESWFRMLELCAAARAEGAQVRPQVAGRPVCLLLGLQTFHPFAYCPSWAPIGGAPIA
                     ERVAAMRDPELRSRLLGEVDVAIAPMRQFLDPERAFPMGTTPNYEPARDDSVAAIAQR
                     QNRPVMEVYYDALMADDGAALIMRPLLNYTDFSLDAVRTMLEHPTSAWGLGDGGAHCG
                     TTCDASTPTFMLSHWSRDRSHDRLPLEWVVRKMTAETASLYGLGDRGRLAPGLLGDVN
                     VVDYENLALPRPQMVHDLPGDARRFVQGSTGYVATIKRGEIVLRDGEDLGGRPGQLIR
                     GAR"
     gene            2822..>3168
                     /locus_tag="VFX21_00140"
     CDS             2822..>3168
                     /locus_tag="VFX21_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094380.1"
                     /translation="MKKVRKIAAFGALVVLAGAIGLAAPAGAVTEVDFLTQCLGKDAN
                     NPAGDDIEPPAAPTVVTVDAPAEVNVGASFTVRAKIDMVPPLPVVANDIKIFIDVLGG
                     GTPAQHVITIPTAT"
BASE COUNT          508 a         1109 c         1075 g          476 t
ORIGIN      
        1 agccggtgtt acacgaccga atcgggccgg cgcgccgtcc tgccgctggc gagcatcggt
       61 tcgggtgcca cgacggcgta cgcgtcaccg cggcgcggtt ggggctacgg cggcctgatc
      121 gccgagggcg gggtcacgcc cgaggacatc gccgtcgcat cggccgacat gtcacgactg
      181 cgtgcgctga agttccacgt gcggcccaac ccgctgcaag cgccgatgtg ggagaagttc
      241 gcgcccgaga tgtgccgcac gcgcaaggaa gtgcacatcc tcgacctccg cggcggcgag
      301 gacgccgcgt tcgagaagct gcacaagagc gcgaagaaag gcatcaaggc agccgagcgc
      361 tgcggtgttc gcatcgagtc cgcgtcggga gaccagctgc tcggcatctt cttcgagctg
      421 acctacgcgg cgcgggcgca atgggccggg cagcaacacg aacccgtatg gctggcacag
      481 ctgcgcggcc gtttccgcga ctccgaacgc aagtggaaga acatcgccaa gcacttcggt
      541 ccgaacctgc gcgtgtacgt cgcgtggtgc gacgaccggc ccgcggcagc cggcatcgtg
      601 ttgcgaggac gcaacgtcca cggcgcccgc gccgcaatgg accgcgaggt cgcgaaccgt
      661 tccggcgcga cgcacgcgct caactgggcc gtcatcaagg acgcgtgcgc gtcgggcgcg
      721 cagtcgttcc acatgggcga gagcgcgacc aagggcgccg ccgacttcaa gcggttcctc
      781 ggtgcggaat gtgtcccgtt cgacgagatc gactacgagc gcttccccct ctcgcgcgcc
      841 aatcgggcca cgaggtcgct cgtcaagaag gtcgtcggct tcaaggaaga cgacgtcaac
      901 gccgtcgtga gctgacggcg ccgcgctacc tgtagcggcg cgagcgcagg atcagcgggc
      961 gcctcggatc agctgtccgg ggcgtccgcc aaggtcttcg ccgtcccgca ggacgatctc
     1021 gccgcgcttg atcgtcgcga catagccggt cgagccctgc acgaaccggc gcgcgtcgcc
     1081 cggcaggtcg tgcaccatct gcggccgcgg cagcgcgagg ttctcgtagt cgacgacgtt
     1141 gacgtcgccg agcaggcccg gcgcgagccg gccccggtcg ccgagcccgt acagcgacgc
     1201 cgtctccgcg gtcatcttgc gcacgaccca ctcgagcggc aggcgatcgt gggaacgatc
     1261 gcgtgaccag tgcgacagca tgaacgtcgg cgtgctcgcg tcgcacgtgg tgccgcagtg
     1321 cgcgccaccg tcgccgagcc cccaggcact cgtggggtgc tcgagcatcg tccgcactgc
     1381 atcgagcgag aagtcggtgt agttcagcag tggacgcatg atcagtgccg cgccgtcgtc
     1441 ggccatcagc gcgtcgtaat agacctccat cacggggcgg ttctgccgct gcgcgatcgc
     1501 ggcgacgctg tcgtcgcgcg cgggctcgta gttgggcgtc gtgcccatcg ggaaggcccg
     1561 ctcggggtcg aggaactgcc gcatcggtgc gatggccacg tcgacctcgc cgagcagacg
     1621 gctgcgcagc tcgggatcgc gcatcgccgc gacccgctcg gcgatcggcg caccgccgat
     1681 cggtgcccac gacgggcagt acgcgaaggg atggaacgtc tgcagcccga gcagcaggca
     1741 caccggccgc cccgcgacct gcggccgcac ctgtgcacct tccgcacggg cggcagcgca
     1801 cagctcgagc atgcggaacc acgattcggg gtcgtggtcg ttctgggtga gcgcgaacgt
     1861 caccggccgg ccgatctcgg cggagagctt gcgcatccac gccatctcgc ggtcgggcgc
     1921 ggcgaggtct tcgccgagcg cacccgcggg tgcgagctcg aacacgccca tgccgagctc
     1981 accgagcacg cggccgatcc cgaacagctc gtcttcggct gcgaacgtgc ccggcaccgg
     2041 ctcgccgtcg atcgccatgt gtgcgatcgt tcggctggtc gagaacccga gcgcgcccgc
     2101 cgcgatgccg tcgcgcacga tgcgcgccat ctcgtcgatg tcgtcgggag tcgccggttc
     2161 gttcttcgcg ccgcgttcac ccatcacgta gccgcgcacc gcgccgtgcg gcacttgcgt
     2221 gccgacgtcg atcatcttgg gcttgcgttc gatcgcgtcg aggtactcgc cgaacgactc
     2281 ccactcccac tcgatgccct cggacagcgc ggcaccggga atgtcctcga cgccttccat
     2341 caacccgatc agccactcgt gccggtcggg atgcaccggc gcgaacccga caccgcagtt
     2401 gcccatgacg acggtcgtca caccgtgcca acacgtcggc gtgagcttcg ggtcccaggt
     2461 gatctgaccg tcgaagtgcg tgtgcacgtc gacgaagccg ggcgtgacga cgagcccgtc
     2521 ggcatcgacc atctcgcgcg ccgcgccgtc gacgcgaccc acctcggtga tcacaccgtc
     2581 ggtgatcgca acgtccgcgg tgcgcggcgc gtcgcccgtt ccgtccacga ccgtgccgcc
     2641 tttgatcacg agatcgtgca cgctgactcc gtttcgaggg ggattcgtat tcgatcacac
     2701 ccccggggtc ggcgacatcc gtcgagactg ggagatttag gtgattcaac ccttttgacg
     2761 atcttgggtt actatgcggt aaccagcggc caccagggga cggccgcgta ggaggggtgg
     2821 gatgaagaag gtccgaaaga tcgctgcgtt cggcgcgctc gtcgtgctcg ccggcgcgat
     2881 aggactcgcc gcacccgcgg gtgcggtcac cgaggtcgac ttcctgacac agtgcctcgg
     2941 caaggacgcc aacaacccgg cgggcgacga catcgaaccg cccgcggcgc cgaccgtcgt
     3001 caccgtggac gcaccggccg aagtgaacgt gggcgcgagc ttcacggtac gggccaagat
     3061 cgacatggtt ccgccgctcc ccgtagtggc gaacgacatc aagatcttca tcgacgtgct
     3121 cggcgggggc acaccggctc agcacgtcat caccatcccg accgcgac
//
LOCUS       DASUGA010000008         3557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1940790, whole genome shotgun sequence.
ACCESSION   DASUGA010000008 DASUGA010000000
VERSION     DASUGA010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1940790"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFX21_00145"
     CDS             <1..217
                     /locus_tag="VFX21_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5094381.1"
                     /translation="HYVPPVWPPVEGEPQMMIHIDIGVEDLDAGVAWALAAGARIAPE
                     QPQKPGEHVIMLDPAGHVFCMCHVASR"
     gene            complement(346..1143)
                     /locus_tag="VFX21_00150"
     CDS             complement(346..1143)
                     /locus_tag="VFX21_00150"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019547849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5094382.1"
                     /translation="MLAAFFDLDKTIIATSSVMALGGPFYREGLISKRTIARGIYAQV
                     IYMLVGADEEKMERMREAMLALTRGWDHEHVKEIVREALEQVITPVIYAEALELIEEH
                     KAKGHKTVIISSSPIETVEAIGAHLGVDDVIATRARLDDEGRYTGELEFYAYGPYKAE
                     AIREMAVREGIDLAGSWAYSDSITDLPMLELVGNPVAVNPDRELARVAADREWETRTF
                     QRPVRLRDRVPVPPKGPTIAAGSAIAAVGAGVLLYMWLRRRAEMART"
     gene            1274..1972
                     /locus_tag="VFX21_00155"
     CDS             1274..1972
                     /locus_tag="VFX21_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094383.1"
                     /translation="MLLACWSPKGGSGTSVFVAACALSAARDGMVRIADLGGDQPAIL
                     GLTEDPPTGLREWLAVGVTAPVDALGRLAVPAGPQLRLLPAGSARVDGLDPEAGAALA
                     VALREETRLTIVDAATADTPALEAVVDVADASVVVVRGCYLALRRAVRRPEVARAAGA
                     VLVEEAGRALGAREVSDVLGIPVLAKVPVRSSTARAIDAGVLAARLPESLMRPARTLL
                     DRIGASGGREGRAA"
     gene            1969..3147
                     /locus_tag="VFX21_00160"
     CDS             1969..3147
                     /locus_tag="VFX21_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX5094384.1"
                     /translation="MTATLDRALRDSVHRRLLASPFDPRAVSRDALRAEIGRILRDEA
                     PLCPHDEARRLVDVLVDEVGGLGPLERWLDDPTVTEIMVNGPGRAYVERAGRLEPIDL
                     PVDGPTIARLVDRVVAPLGLRLDHASPMVDARLPDGSRLHAVLPPLAPDGPCVTIRRF
                     TVRDATLATFGVDAEAAVKLRALVRCGWNLLVAGATSAGKTTLCNTLAASIEAGDRIV
                     TIEETAELRLPQPHVVRLEARPANAEGVGAVGVRDLVRAALRMRPDRLVVGEVRGGEA
                     LDMLQALNTGHDGSLSTIHANGPDEALARLETLALLGGVALPLSAIRAQIGAAVDGVV
                     QVRRTGDGTRVVTAVAEVDPTGTSTRPLLERRNDALCACGAPARPARRLALALREAWS
                     "
     gene            3144..>3557
                     /locus_tag="VFX21_00165"
     CDS             3144..>3557
                     /locus_tag="VFX21_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094385.1"
                     /translation="MIATLCVGVAGAATSTALWRASRRFAVRDRLHSTPTRFVRPAVV
                     RRWLVRALDSAAIDTTPEHALQTWGLALLIAAVLGLGLGPTTGLLMVLGVAVGGPAWL
                     HANRHRRARAATTAIPEFLDQVASELRAGGTVATAI"
BASE COUNT          452 a         1222 c         1292 g          591 t
ORIGIN      
        1 gcactacgtg cccccggtct ggccgccggt cgagggcgag ccgcagatga tgatccacat
       61 cgacatcggc gtggaggacc tcgacgccgg tgtcgcgtgg gcgctcgcgg cgggcgcgcg
      121 gattgcaccg gagcaaccgc agaaaccggg cgaacacgtc atcatgctgg atccggccgg
      181 ccacgtcttc tgcatgtgcc acgtcgcgtc gcgctagggt ccgaaacgcc tgttggcgga
      241 gagggagcgt tccgtgaaga agatcgtgct gctgcttgtg ctcgttgcgt tgggtgccct
      301 cgtcgcgaag aaggttcgct ccgcctgatc agctcgtcag ctgcgtcacg tacgcgccat
      361 ctcggcgcgc cgtcgcaacc acatgtagag gagcacaccc gcgccgacgg ccgcgatcgc
      421 gctgccggcg gcaatcgtcg gtcccttggg cggcaccggg acccggtcgc gcagtcgtac
      481 cgggcgctgg aatgtgcgcg tctcccactc gcggtcggcg gcgacgcgcg cgagctcgcg
      541 atcggggttg accgcaaccg ggttgccgac gagctcgagc atcggcaagt cggtgatcga
      601 gtcggaatat gcccacgacc ctgcgaggtc gatgccttcg cgcaccgcca tctcccggat
      661 cgcttctgct ttgtacggac cgtacgcgta gaactcgagc tcacccgtgt agcggccctc
      721 gtcgtcgagc cgggcccgcg tcgcgatcac gtcgtcgacc ccgaggtgcg cgccgatcgc
      781 ttcgacggtt tcgatcggcg acgacgagat gatcacggtc ttgtggcctt tggccttgtg
      841 ctcttcgatg agctcgagcg cctcggcgta gatgaccggc gtgatcactt gctcgagcgc
      901 ttcgcgcacg atctccttca cgtgctcgtg atcccatccg cgcgtcagcg cgagcattgc
      961 ttcgcgcatg cgttccatct tttcctcgtc ggcgccgacg agcatgtaga tgacctgcgc
     1021 gtagatcccg cgcgcgatcg tgcgcttcga gatcaagcct tcgcgataga acggcccgcc
     1081 gagcgccatc accgaactgg tggcgatgat cgtcttgtcg agatcgaaga acgcggccaa
     1141 catcgacccg tagcgtattt cgcgcgcctg ttgacgcgcg gtcgtcggtg ccataagttc
     1201 gtgcgcactt ctcctcgccc ttccccggca tccacattgg tgtctcgcca ttgatgtctc
     1261 gggggagggc gttgtgctgc tcgcatgctg gtcgccgaag ggcggctcgg gtacgtcggt
     1321 cttcgtcgcg gcctgcgcgc tgagcgcggc gcgcgacggg atggttcgga tcgcggatct
     1381 gggcggcgac caaccggcga ttctcggcct caccgaagat ccaccgaccg gcctgcgcga
     1441 gtggctcgcg gtcggcgtca cggcaccggt cgacgcactc ggccgcctgg cggtgcctgc
     1501 gggtccgcag ctgcgcctgc tcccggccgg atccgcgcgc gtcgacggtc tcgatcccga
     1561 ggcgggcgcg gcactggccg tcgcactgcg cgaagagaca cggctgacga tcgtcgacgc
     1621 cgcgaccgct gacacgcccg cgctcgaggc ggtcgtcgac gttgccgacg cgagtgtcgt
     1681 cgtcgtacgc gggtgctatc tcgctctccg ccgcgccgtg cggcggcctg aggttgcgcg
     1741 cgcggccggc gcggtcctgg tcgaggaagc cgggcgtgcg ctcggcgcgc gcgaagtgag
     1801 cgatgtgctc gggattccgg tgttggcgaa ggtgccggtg cgatcgtcga cggcgcgggc
     1861 gatcgacgcc ggcgtgctcg cggcgcggct gccggaatcg ctcatgcgtc ccgcccgaac
     1921 gttgctcgac cgcatcggcg caagcggtgg tcgtgaagga cgcgccgcat gaccgcgacg
     1981 ctcgatcgcg ccctgcgcga ttcagtgcac cggcgcctgc tcgcgtcgcc gttcgatccg
     2041 cgcgcagtca gccgtgacgc gctgcgggcc gagatcggtc gcatcctccg cgacgaagcg
     2101 ccgctgtgcc cgcacgacga ggcgcggcgg ttggtcgacg tgctcgtcga cgaggtcggc
     2161 gggctcggcc cgctcgaacg ctggctcgac gacccgaccg tcacagagat catggtgaac
     2221 gggcccgggc gcgcgtacgt cgagcgggcc ggccggctcg agccgatcga cttgcccgtc
     2281 gacggcccga cgatcgcacg tctcgtcgac cgggttgtcg cgccgctcgg actgcgcctc
     2341 gaccacgcgt cgccaatggt cgacgcgcga ctgcccgatg gctcgcgatt gcacgccgtg
     2401 ttgccgccgc tcgcgcccga cggcccgtgc gtgacgatcc ggcgcttcac ggtgcgtgat
     2461 gcgacgcttg cgacattcgg cgtcgacgcg gaggccgcgg tcaagctgcg tgcgctcgtg
     2521 cggtgtggtt ggaacctcct cgtcgcgggc gcgacgagcg cgggcaagac gacgttgtgc
     2581 aacacgctgg cggcatcgat cgaagcgggc gatcggatcg tgacgatcga ggagacggcc
     2641 gagctgcgtt tgccgcaacc gcatgtcgtg cgcctcgaag cgcgccccgc gaacgccgaa
     2701 ggcgtgggcg cggtcggcgt tcgcgacctc gtgcgcgccg cgttgcgcat gcggcccgac
     2761 cggctcgtcg tcggtgaggt gcgcggcggc gaagcactcg acatgttgca ggcgttgaac
     2821 accggccacg acggttcgtt gtcgaccatc cacgccaatg gccccgacga agcgctcgcg
     2881 cgcctggaga cgctggcgct gctgggcggg gtggcgctgc cgttgtcggc gattcgggcc
     2941 cagatcggag cggccgtcga cggcgtcgtg caggtgcgcc ggaccggcga cggcacgcgc
     3001 gtggtcaccg cagtcgccga agtggatccg acggggacgt cgacgcggcc gttgctcgaa
     3061 cggcgcaacg atgcgttgtg cgcgtgtggc gcgcccgcgc gcccggcgcg ccggctcgct
     3121 ctcgcgctgc gggaggcgtg gtcgtgatcg cgacgctctg tgtcggcgtc gccggtgcgg
     3181 ccacgagcac cgcgctgtgg cgcgcgagcc ggcggttcgc agttcgcgat cggttgcact
     3241 cgacgccgac gcggttcgta cgcccggccg tcgtccgccg gtggttggtg cgcgcgctcg
     3301 acagcgcggc gatcgacacc acgcccgagc acgcgctgca gacgtggggc ctcgcgctgt
     3361 tgatcgcggc ggtactcggc ctcgggctgg gcccgacgac gggcttgctc atggtgctcg
     3421 gcgtcgccgt cggcgggccg gcgtggttgc acgccaatcg tcaccggcgg gcccgtgcgg
     3481 ccacgactgc cattcccgag ttcctcgacc aagtggcgtc ggagctgcgc gcgggcggca
     3541 ccgtcgcgac tgcgatc
//
LOCUS       DASUGA010000009         2337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1939489, whole genome shotgun sequence.
ACCESSION   DASUGA010000009 DASUGA010000000
VERSION     DASUGA010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1939489"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VFX21_00170"
     CDS             complement(<1..666)
                     /locus_tag="VFX21_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5094386.1"
                     /translation="MIHDAELFTVGCDEAVVTFRTDEDATVVTVVGEHEVSTSGRLHA
                     ARVTGLEAATEYPLRVDGVDASELLPAVLTTLPRPSGRRVATFATVNDVHFGEFECGK
                     LGTPEELGPVFSTEPGDTPYYEVMNRGAIAEIAALDPDAVVVKGDLTNLGEEWEYELF
                     CNAYTQLGARMHHVRGNHDAMLSAKIAATGPFTVELDGITLAVLDTVIPGHENGQVTP
                     EQFS"
     gene            complement(691..912)
                     /locus_tag="VFX21_00175"
     CDS             complement(691..912)
                     /locus_tag="VFX21_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094387.1"
                     /translation="MSPRSTPPPDHVAAIAAAMAVISAERAAVSADASSPSDRLTMWV
                     EASRRSAQRAGLQRGPWRMSGRISRRLRV"
     gene            complement(909..>2337)
                     /locus_tag="VFX21_00180"
     CDS             complement(909..>2337)
                     /locus_tag="VFX21_00180"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX5094388.1"
                     /translation="EFLLDEGSFHELDMLARHRAHGFGIEQNRPFTDGVVTGWGKIDG
                     RKVFVFSQDFTLFGGALGEVFAEKIHKVMDLAESVGAPLIGLNDGAGARIQEGVVSLA
                     SYGGIFYRNVKASGVIPQISVILGPCAGGAVYSPAMTDFIFMVTGTSHMFITGPDVVK
                     TVTGEEVTQEELGGAMTHATKSGVATFVAPDEQTCLEQVRYLVSFLPSNNLEDPPYFE
                     PTDDAERSCDALIDLMPDSPNRPYDMKKVIAEIVDDQEFFEVFPLWAMNIVCGFARID
                     GYTVGIVGNQPQVLAGVLDIDASEKAARFVRTCDAFNVPMLTFVDVPGFLPGTDQEYG
                     GIIRHGAKLLYAYCEATVPRVQVITRKGYGGAFVVMNSKSIGADVAFAWPSAEIAVMG
                     PAGAVEIIFRREIQMAEDPVQRQAELVQEYTERFANPYQAAERGYVDDVIDPRDTRKM
                     LINAFAMLRTKREQLPQRKHGNGPL"
BASE COUNT          370 a          785 c          788 g          394 t
ORIGIN      
        1 cgagaactgc tcgggcgtca cctgaccgtt ctcgtggccg ggaatgacgg tgtcgagtac
       61 ggccagcgtg atgccgtcga gctcgacagt gaacggaccg gtcgccgcga tcttcgcgct
      121 gagcatcgcg tcgtggttgc cccgcacgtg gtgcatgcgc gcgcccagtt gcgtgtacgc
      181 gttgcagaac aactcgtact cccattcctc accgaggttg gtgaggtcgc ccttgacgac
      241 gacggcatcg ggatcgagcg cggcgatctc ggcgatggcg ccacggttca tgacctcgta
      301 gtacggcgtg tcgccgggtt cggtgctgaa caccgggccc agctcttcgg gcgtaccgag
      361 cttgccgcat tcgaactcgc cgaagtgaac gtcgttcacg gttgcaaacg tcgcgacgcg
      421 ccggcccgac gggcggggca gtgtcgtcag caccgccggg aggagctcgc tcgcatccac
      481 gccgtcgacg cgcagcgggt actcggtcgc cgcttccagc ccggtcaccc gggccgcgtg
      541 gagcctgccg ctcgtcgaca cctcgtgctc gccgacgacg gtaacaaccg tcgcgtcctc
      601 gtcggtgcgg aaggtgacga cggcttcgtc acatccgacc gtgaacagct cggcgtcgtg
      661 gatcacgaat gtgagactag gccgcgccgg ctacacgcgg agtcgccgac tgatgcggcc
      721 gctcatgcgc cacgggccgc gttgcagacc ggcgcgctgc gcgctgcggc gcgacgcttc
      781 gacccacatc gtcagacggt cactcggcga cgacgcgtcc gccgagaccg cggcgcgctc
      841 agcgctgatg acggccatcg ccgccgcgat cgcggcgacg tggtcgggtg gcggcgtcga
      901 ccgcgggctc acagcggccc gttgccgtgc ttgcgctgcg ggagctgctc gcgcttggtc
      961 cgcagcatcg cgaacgcgtt gatcagcatc ttgcgggtat cgcgcgggtc gatgacgtcg
     1021 tcgacgtagc cgcgctcggc cgcctggtac gggttcgcga agcgctcggt gtactcctgc
     1081 acgagctcgg cctggcgctg caccggatcc tcggccatct ggatctcacg gcggaagatg
     1141 atctcgaccg cgccggccgg ccccatcacc gcgatctccg ccgagggcca cgcgaacgcg
     1201 acgtcggcgc cgatcgactt ggagttcatg acgacgaacg cgccgccgta gcccttgcgc
     1261 gtgatgacct gcacccgcgg caccgtcgct tcgcagtacg cgtacaagag cttggcgccg
     1321 tgccggatga tgccgccgta ctcctggtcg gtgccgggca ggaagccggg gacgtcgacg
     1381 aacgtcagca tcggcacgtt gaacgcgtca cacgtgcgca cgaagcgcgc cgccttctcg
     1441 gacgcgtcga tgtcgagcac gcccgccagc acctgcggtt ggttgccgac gatgccgacc
     1501 gtgtacccgt cgatgcgcgc gaacccgcag acgatgttca tggcccagag cgggaagacc
     1561 tcgaagaact cttggtcatc gacgatctcg gcgatgacct tcttcatgtc gtacggccgg
     1621 ttcggcgagt cgggcatgag gtcgatcagc gcgtcgcacg agcgctcggc atcgtcggtc
     1681 ggctcgaagt acggcgggtc ctcgaggttg ttcgacggca ggaacgacac cagatagcgc
     1741 acctgctcga ggcacgtctg ctcgtcgggt gccacgaacg tcgcgacacc cgacttcgtc
     1801 gcgtgcgtca tcgcgccgcc cagttcttct tgcgtcacct cttcgcccgt gacggtcttc
     1861 acgacatcgg ggccggtgat gaacatgtgt gacgtgccgg tgaccatgaa gatgaagtcg
     1921 gtcatcgcgg gcgaatacac ggcaccaccg gcgcacggcc cgaggatcac gctgatctgc
     1981 gggatgacgc ccgatgcctt gacgttgcgg tagaagatcc cgccgtacga cgcgagtgag
     2041 acgacgccct cctggatgcg cgcacccgca ccgtcgttga gcccgatgag cggtgcgccg
     2101 accgactcgg cgagatccat gactttgtgg atcttctcgg cgaacacttc gccgagcgcg
     2161 ccaccgaaga gtgtgaagtc ttgtgagaag acgaacacct tgcggccgtc gatcttgccc
     2221 caccccgtga cgacaccgtc ggtgaacgga cggttctgct cgataccgaa gccgtgcgcg
     2281 cggtggcggg ccagcatgtc gagctcgtgg aaggagccct cgtcgagcag gaactcg
//
LOCUS       DASUGA010000010         4043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_968249, whole genome shotgun sequence.
ACCESSION   DASUGA010000010 DASUGA010000000
VERSION     DASUGA010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968249"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VFX21_00185"
     CDS             <1..244
                     /locus_tag="VFX21_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5094389.1"
                     /translation="RALGLDIDAKPTFSEVMAAREDRQAKVAAVIDGLTPEALLRQCP
                     HMDRGRPTVQTCLHVVLREEFAHNRYANRDLDQLAS"
     gene            313..1503
                     /locus_tag="VFX21_00190"
     CDS             313..1503
                     /locus_tag="VFX21_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011605970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HEX5094390.1"
                     /translation="MQDVHAPPTVDARSEYFDVRAPAVDIVVPVYNEQAVLDASVRRL
                     HEYLTSHFPFTWRITIVDNASTDDTLHRALRLARDLPRVNAMHLDQKGRGLALRTAWS
                     ASDAAVVAYMDVDLSTDLDALLPLVAPLKSGHSDIAIGSRLALGSSVARGPQRELISR
                     GYNAMLRTMFAARFRDAQCGFKAVRADVARRLLPAIVDNGWFFDTELLLLAEHNGLRV
                     HEVPVDWIDDPDSRVDVAHTALHDLKGAARMARTFLSGGGRIDLGPAARSPLADDFGR
                     SIVRFGLIGLASTAVSLTIFLLLHDALGAVAANAVAVLSTFVANTWANARYTGYRARP
                     RWLAATGLILLALALTSVGLLAVGALGGGLGWELAVLLCTWTLAGALRLAAIRSWSRA
                     EVHA"
     gene            1541..3619
                     /locus_tag="VFX21_00195"
     CDS             1541..3619
                     /locus_tag="VFX21_00195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014440630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5094391.1"
                     /translation="MTDVLTPPAFERAEPAPPVTPPAGPPATSCWRRVWRGPERDPAW
                     ARPSLFVLLAATGVLYLWHLGDNGWANTYYSAAVQAGTQSWKAFFFGSLDAANSITVD
                     KAPASLWVMELSARVFGVNSWSILVPQALEGVATVGVLYMTVKRWFSPGAALLAGAVV
                     AVTPVAALMFRFNNPDALLVLLLVLGAYALTRALERGSTWWLVLAFSFVGFGFLAKML
                     QALVVVPVFGVVYLLCGPPKLGRRIGQLALGAVALLVSAGWWVAIVELWPKSARPYIG
                     GSENNSVLNLIFGYNGFGRLNGDETGSVGGGPAGTSGRWGPTGWNRLFNSSWGAEASW
                     LIPAALLLGAVVLIATLNRPRTDRTRAALLLWGGWLVVTGAVFSFGQGIIHEYYSVAL
                     APAIGAVVGISAFHLWERRAQFYARAALAAGVAITAWWSTQLLHRVPDWHPGLQSVVM
                     IGGGAVAVALLVMPLMDRKIVPLVGLTAIAVCLLGSSAFALSTANEPHSGAIPLSGPQ
                     RSAGAGGPPQFFRPQQSLGNRQQPFGQQPRQFGQPQPFRGGQAPNAGANRGTLGGLLE
                     GSEPSETLQALLSENADEYMWVAATTGANTAAGYQLATGKPVMALGGFNGTDPSPTLE
                     QFQQYVADCQIHYFIGGGGFGGVMRFGTFGGANANTSSEIARWVEENFTAQTVGGATV
                     YDLSGTTSAQ"
     gene            complement(3586..3927)
                     /locus_tag="VFX21_00200"
     CDS             complement(3586..3927)
                     /locus_tag="VFX21_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094392.1"
                     /translation="MPDLVRDRSKQLLGGMYRAEIIAAINEIGDRSFTLVTLEDELAC
                     TGVRVPPSLLHKEVTILLSCDLLDRHGRNETGHYVYTRRRWEAFWTTMACFLQLQLPE
                     DYCAEVVPLRS"
BASE COUNT          572 a         1413 c         1375 g          683 t
ORIGIN      
        1 acgcgcgctc ggcctcgaca tcgatgcgaa gccgacgttc tccgaggtga tggcggcacg
       61 cgaagaccgc caggcgaaag tcgccgccgt catcgacggc ctcacacccg aagcgctgct
      121 gcggcaatgc ccgcacatgg accgcggccg accgacggtg cagacctgcc tccacgtcgt
      181 gctgcgcgag gagttcgccc acaaccggta cgcgaaccgg gacctcgacc agctcgcttc
      241 ctgagaacca ctcacagcaa acactcactc tgctcggaga gggcccagag gtggcggtgc
      301 gatcgtgcgc acatgcagga cgtgcacgcg ccgccgacgg tcgacgcccg ctccgagtac
      361 ttcgacgtcc gggcgcccgc ggtcgacatc gtcgtgccgg tctacaacga gcaggcggtc
      421 ctggacgcca gcgttcgccg cctccacgag tacctgacct cgcatttccc gttcacctgg
      481 cgcatcacga tcgtcgacaa cgcatcgacc gacgacaccc tgcaccgggc cctgcgcctg
      541 gcccgcgacc tgccgcgcgt gaacgcgatg catctcgacc agaagggccg cggccttgcg
      601 ctgcgcacgg catggagcgc gagtgacgcg gccgtcgttg cctacatgga cgtcgacctg
      661 tcgaccgatc tcgacgcgct gttgcccctc gtcgcgccac tcaagtcagg gcactccgac
      721 atcgcgatcg gttcccggct cgcgctgggt tcctcagtcg cacgaggacc ccagcgcgag
      781 ctgatctcgc gcgggtacaa cgcgatgctg cgaacgatgt tcgcggcgcg cttccgtgac
      841 gcgcagtgcg ggttcaaggc cgtgcgtgcc gacgtcgcgc gccggttgct gccggccatc
      901 gtcgacaacg gctggttctt cgacaccgaa ctgttgttgc tcgccgagca caacggcctg
      961 cgcgtgcacg aggtaccggt cgactggatc gacgatcccg acagccgggt cgacgtcgcg
     1021 cacacggccc tgcacgacct gaagggcgcg gcgcgcatgg cacgcacgtt cctctccggc
     1081 ggcggaagga tcgacctcgg ccccgcggcc cggtcacctc tcgccgacga cttcggccgc
     1141 agcatcgtcc gcttcggcct catcgggttg gcgagtacgg cggtgtcgtt gacgatcttc
     1201 ttgctgctgc acgacgcgct cggcgccgtc gccgccaacg cggtcgcggt gctctcgacg
     1261 ttcgtcgcca acacatgggc caatgctcgc tacaccggct atcgggcgcg gccgcgctgg
     1321 ctcgcggcca ccgggctgat cctgctcgcg ctcgcactga cgagcgtcgg cttgcttgcc
     1381 gtcggcgcac tcggcggtgg gctcggctgg gagctcgcag tgttgctgtg cacctggacg
     1441 ctcgccggcg cgctgcgact ggcggcgatc aggtcgtggt cgcgcgccga ggtgcacgcg
     1501 tgatcgccac gccgtctgtc gtcttccggg ggtcgcccgc atgaccgatg tcctgacgcc
     1561 ccccgcattc gagcgggccg agccggcgcc gcccgtcacg ccgccggccg gcccgcccgc
     1621 cacttcctgc tggcgccgcg tgtggcgcgg tcccgagcgc gatcccgcgt gggcgcgtcc
     1681 gtcgctgttc gtgctgctcg cggccaccgg cgtgctgtac ctgtggcacc tcggcgacaa
     1741 cggttgggcc aacacctatt actccgccgc cgtgcaagcc gggacgcaga gctggaaggc
     1801 gttcttcttc ggttcgctcg acgccgcgaa ctcgatcacg gtcgacaagg cgcccgcgtc
     1861 gctctgggtg atggaactga gcgcgcgcgt cttcggcgtg aactcgtgga gcatcctcgt
     1921 gccgcaggcg ctcgaaggtg tcgcgacggt cggtgtgctc tacatgacag tgaagcgctg
     1981 gttctcgccc ggcgccgcgt tgctcgcggg cgcggtcgtc gcagtgacac ccgtcgcggc
     2041 gttgatgttc cggttcaaca atcccgatgc gttgctggtg ctgttgctcg tgctcggtgc
     2101 gtacgcactc acgcgtgcgc tcgagcgcgg cagcacctgg tggctcgtgc tcgccttctc
     2161 gtttgtcggg ttcgggttcc tcgccaagat gctgcaggcg ctcgtcgtgg tgccggtgtt
     2221 cggtgtcgtc tacctgctgt gcgggccgcc gaagctcggc cggcgcatcg ggcaactcgc
     2281 cctcggcgcg gtcgcgctcc tcgtgtcggc cggttggtgg gtcgcgatcg tcgagctgtg
     2341 gccgaagagc gcgcgcccgt acatcggcgg ctcggagaac aacagcgtcc tcaacctgat
     2401 cttcggttac aacgggttcg ggcggctcaa cggcgacgag acgggcagcg tcggcggtgg
     2461 gcccgcgggc accagcgggc gttgggggcc gacggggtgg aaccgcctct tcaacagcag
     2521 ctggggcgcc gaagcatcgt ggctcatccc cgccgcgttg ttgctcggcg cagtcgtgct
     2581 catcgcgacg ttgaaccggc cgcgcaccga tcgcacgcgc gccgcgctgc tgttgtgggg
     2641 cggctggctc gtcgtgacgg gcgccgtgtt cagcttcggg cagggcatca tccacgagta
     2701 ctactcggtt gcgctcgcac ccgcgatcgg cgcggtggtc ggcatcagcg cgttccactt
     2761 gtgggaacga cgcgctcagt tctacgcgcg cgccgcgctc gcggcgggag tcgcgatcac
     2821 ggcatggtgg tcgacgcaat tactgcaccg cgtccccgac tggcacccgg ggctgcagtc
     2881 ggtcgtgatg atcggtggtg gcgcggtcgc cgtcgccctg ctcgtgatgc cgctcatgga
     2941 ccgcaagatc gtgcccctcg tcggcctcac cgcgatcgcg gtgtgcctgc tcgggtcgtc
     3001 cgcgttcgcg ctctcgaccg cgaacgagcc acacagtggt gcgattccgc tgtccgggcc
     3061 gcagcgcagt gcaggtgccg gcggaccacc gcagttcttc cgtccccaac agtcactcgg
     3121 caaccggcaa cagccgttcg gacaacaacc gcgtcagttc gggcagccgc aaccgttccg
     3181 cggcggacaa gcaccgaacg cgggcgcgaa ccgcggcacg ctcggcggac tgctcgaagg
     3241 cagtgaaccg agcgagacgt tgcaagcgct gttgtcggag aacgccgacg agtacatgtg
     3301 ggtcgcggcg acgaccggcg ccaataccgc ggccggctac caactcgcga ccgggaagcc
     3361 cgtgatggca ctcggcggct tcaacggcac cgatccgtcg ccgacgctcg agcagttcca
     3421 gcaatacgtc gccgactgcc agatccacta cttcatcggc ggcggtgggt tcggcggcgt
     3481 gatgcgcttc ggcactttcg gcggcgccaa cgcgaacacg tcgtcggaga tcgcgcgctg
     3541 ggtcgaggag aacttcacgg cgcagaccgt cggcggcgcg accgtctacg acctcagcgg
     3601 cacgacttcc gcgcagtagt cctccggcag ttgcagctgg aggaagcagg ccatcgtcgt
     3661 ccagaacgct tcccaacggc gacgtgtgta cacgtagtga ccggtctcgt tgcggccgtg
     3721 ccggtcgagc aagtcgcacg acagcaggat cgtcacttcc ttgtggagca gtgacggcgg
     3781 cacgcgcacg ccggtgcacg cgagctcgtc ttcgagggtg acgagcgtga acgaccggtc
     3841 gccgatctcg ttgatggcgg cgatgatctc ggcgcggtac atcccaccga gcagctgctt
     3901 cgaacggtcg cggacgagat cgggcacccg gcctcctggg catccagggt tgccggcccc
     3961 taatccgcga ctcgcgaatc gtaggccagg gaattgtcat tcggtggtca ttcccgctcc
     4021 ggcagtagtt cgggggtcta acc
//
LOCUS       DASUGA010000011         3605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1939381, whole genome shotgun sequence.
ACCESSION   DASUGA010000011 DASUGA010000000
VERSION     DASUGA010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1939381"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..438
                     /locus_tag="VFX21_00205"
     CDS             13..438
                     /locus_tag="VFX21_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5094393.1"
                     /translation="MTDAQHVADLVELHALCARYMMLTSQYIQDRWLEVFTPDGEYNA
                     FGTPYTLERFPALLEAAPRGQFIGNMPVVEIDGDTASGVQHFVFFDQSNHNVRLGWYK
                     DEYRRTADGWRIHRRSTTFMRKHGGFDSGIQHDPLAETQ"
     gene            435..1670
                     /locus_tag="VFX21_00210"
     CDS             435..1670
                     /locus_tag="VFX21_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014054134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5094394.1"
                     /translation="MTDLADLPRIISVDDHVVEPAHLWQDRLPAKMRERGPRIERARW
                     GDIVLGAGASYKQEQTDDGRWGDYWMYEDRLIYLHKRHVTIPLEATPDGDTNRFDRSK
                     LTLNGITYDEMRPGCYEPKARVDDLALAGVDGSLAFPTFPRFCGQTFLEGNDHELGLE
                     CVRAYNDWMIDEWCADSGGTLIPLCLIPLWDVHLAVDEINRNAARGAHAVCFSEIPTH
                     LGLPSIHTGDWDPFFAACEETRTTVCMHIGSSSKMPAASPDAPPTTETMLGFYNSMAS
                     LGDYLFSGVLVRFPQLKLAYSEGQIGWIPYALERADNVWEYHAAWTNTKNTIPEKPSS
                     YYRGRIFGCFTTDLHGIKSIDDVGEDNICFETDYPHTDTTWPFALQEAARMTAGLTEQ
                     QRYKILRGNAIRMLELDRV"
     gene            1681..2622
                     /locus_tag="VFX21_00215"
     CDS             1681..2622
                     /locus_tag="VFX21_00215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016705579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03564 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX5094395.1"
                     /translation="MRIGLMIGPDRGHKPTKAQRMVDAAVKAEEQGFASIWVPQIPYD
                     FDALTAIALMGNATSRVELGTAVMVLQTRHPIAMAQQVLSNQAICAGRFTLGLGPSHH
                     WIVEDMLGLSYEKPAQQVRDYLDVLEAALAGPGRVDVDNDGWRVHNDLDITDYRTPVL
                     LAALAPVMLRIAGERANGTILWMADERAISDHVVPRITKAAADAGRPAPRVVAGIPVV
                     LCRNEEVDGARELANEVLGHADYSPNYQRLLEHGDATDVGDMLAGGDEKSIAERINRF
                     RDAGVTDLSVRVLPLGTNREEKLASWARTQAFVASLC"
     gene            complement(2619..2918)
                     /locus_tag="VFX21_00220"
     CDS             complement(2619..2918)
                     /locus_tag="VFX21_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEX5094396.1"
                     /translation="MPTEHDVTTETLVLKLERQTHTLDYQAGDTVLEAARRGGLNPPF
                     SCEQGNCATCMAHLDDGSVRMKANNALTEEDVEEGWILTCQSLPTSKTLTVDYDA"
     gene            3006..>3605
                     /locus_tag="VFX21_00225"
     CDS             3006..>3605
                     /locus_tag="VFX21_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5094397.1"
                     /translation="MVDDARWPVRSVPRPLREQYVAEGWWTNATLGALVDRSLRATPG
                     AGVHIWSQTRPWHGTYADLLTDAQRLVSALRAAGIAPGDAVAFQLPNWREAVVAFYGL
                     ALGGYVLVPIVHIYGAKEVRFILAQSGARAYISADRYGTVDYVDIVEGADAGALAKLD
                     LHVVVGDASTPGAHVTRIAWDTVDAAPPASDVVASDPDDV"
BASE COUNT          599 a         1250 c         1197 g          559 t
ORIGIN      
        1 cgagttcgaa acgtgaccga cgcgcagcac gtcgcggacc tcgtcgagct gcacgcgctg
       61 tgtgcgcgct acatgatgct gacgagccag tacatccagg atcgctggct cgaagtgttc
      121 acgcccgacg gcgaatacaa cgcgttcggc acgccgtaca cgctcgaacg cttcccggcg
      181 ttgctcgagg cggcaccgcg cggccagttc atcggcaaca tgcccgtcgt cgagatcgac
      241 ggcgacaccg cgagcggcgt gcagcacttc gtgttcttcg accagtcgaa ccacaacgtg
      301 cgcctcggct ggtacaagga cgaatatcgg cgcacggccg acggctggcg catccacagg
      361 cggtcgacga cgttcatgcg caagcacggt gggttcgact ccggcatcca gcacgatccc
      421 ctcgcggaga cgcagtgacc gacctcgccg acctgccccg gatcatcagc gtcgacgacc
      481 acgtcgtcga gcccgcgcat ctctggcagg accgcctgcc cgcgaagatg cgcgagcgcg
      541 gcccgcgcat cgagcgcgcg cgctggggcg acatcgtgct gggtgcgggc gcgtcgtaca
      601 agcaggagca gaccgacgac ggccggtggg gcgattactg gatgtacgag gaccgcctca
      661 tctacctcca caagcgccac gtgacgatcc cgctcgaggc gacgcccgac ggcgacacga
      721 atcgcttcga ccgcagcaag ctgacgctga acgggatcac ctacgacgag atgcggccgg
      781 gttgttacga gccgaaggcc cgcgtcgacg acctcgcgct cgcaggtgtc gacggctcgc
      841 tcgcgttccc gaccttccca cgtttctgcg gacagacgtt cctcgaaggc aacgaccacg
      901 agctcggcct cgagtgcgtg cgcgcgtaca acgactggat gatcgacgag tggtgcgcag
      961 attccggtgg caccctcatc cccctgtgcc tcatcccgct gtgggacgtg cacctcgcag
     1021 tcgacgagat caaccgcaat gcggcccgcg gcgcgcacgc agtgtgcttc agcgagatcc
     1081 cgacgcacct cggcctgccc agcatccaca ctggggactg ggacccgttc ttcgcggcgt
     1141 gtgaagagac gcgcacgacg gtgtgcatgc acatcgggtc gtcgtcgaag atgccggccg
     1201 cgtcaccgga cgcgccgccg acaaccgaga cgatgctcgg tttctacaac tcgatggcgt
     1261 cgctcggcga ctacctgttc tcgggtgtgc tggtccgctt cccgcagctg aagctggcgt
     1321 actccgaagg ccagatcggc tggatcccgt acgcgctcga acgcgccgac aacgtgtggg
     1381 agtaccacgc cgcgtggacg aacacgaaga acacgatccc cgagaagccg tcgagctact
     1441 accgcggccg catcttcggg tgcttcacga ccgacctgca cggcatcaag tcgatcgacg
     1501 acgtcggcga ggacaacatc tgcttcgaga ccgactaccc gcacaccgac acgacgtggc
     1561 cgttcgcgtt gcaggaagct gcgcgcatga ccgccggtct caccgagcag cagcgttaca
     1621 agatcctgcg cggcaacgcg atccgcatgc tcgagctcga ccgggtctag ggagcacgag
     1681 gtgcgcatcg gcctgatgat cggcccggac cggggccaca agccgaccaa ggcccagcgc
     1741 atggtcgacg ccgcggtgaa ggcggaggag caaggcttcg cgtcgatctg ggtgccgcag
     1801 atcccctacg acttcgacgc gctcaccgcg atcgcgttga tgggcaacgc gacctcgcgc
     1861 gtcgagctcg gcaccgcggt gatggtcttg cagacgcggc acccgatcgc gatggcgcaa
     1921 caggtcctgt cgaaccaggc gatctgcgcg ggccggttca cgctcggtct cggcccgtcg
     1981 caccactgga tcgtcgagga catgctcggc ctctcgtacg agaagcccgc gcaacaggtg
     2041 cgggactatc tcgacgtcct cgaggccgcg ctcgcaggtc cgggccgggt cgacgtcgac
     2101 aacgacggct ggcgcgtgca caacgacctc gacatcaccg actaccggac gccggtgttg
     2161 ttggccgcgc tggcgccggt catgctgcgc atcgcgggcg agcgcgcgaa cggcacgatc
     2221 ctgtggatgg cggacgagcg cgcgatcagc gaccacgtcg tcccccgcat caccaaggcc
     2281 gcggccgacg cgggccggcc ggcgccgcgt gtcgtcgcgg gcattcccgt cgtgctgtgc
     2341 cgcaacgagg aagtcgacgg cgcgcgcgag ctcgccaacg aagtgctcgg ccacgccgac
     2401 tactcgccca actaccaacg cctgctggag cacggcgacg ccaccgatgt cggcgacatg
     2461 ctcgcgggcg gtgacgagaa gtcgatcgcc gagcgcatca atcggttccg cgacgccggc
     2521 gtcaccgacc tgtcggtgcg cgtgctcccc ttgggcacca accgcgaaga aaagctcgcg
     2581 agctgggcca gaacccaagc attcgtcgcg tcgctctgtt aggcgtcgta atcgaccgtc
     2641 agcgtcttgc tggtcgggag tgactggcag gtgaggatcc agccctcttc gacgtcttct
     2701 tccgtcagcg cgttgttggc cttcatgcgg accgagccgt cgtcgaggtg ggccatgcac
     2761 gttgcgcagt tgccctgctc gcacgagaac ggcgggttca ggccgccgcg gcgcgccgcc
     2821 tccagcaccg tgtcgcccgc ttggtagtcg agcgtgtgcg tctggcgctc gagcttgagg
     2881 accagcgtct cggttgtgac gtcgtgttcc gtcggcatgg gctccgggcg ggcgctggcg
     2941 agaagcccgc tctccaacta tgttagtggc attctcaccc gcccgggtag gaggaaacac
     3001 gtcgcatggt ggacgacgcg cggtggcccg tgcgttcggt gccccggccg ctccgggagc
     3061 agtacgtcgc cgagggatgg tggacgaacg ccacgctcgg cgcgctcgtc gaccgatccc
     3121 tgcgcgccac gcccggcgcc ggtgtccaca tctggtcgca gacacggccg tggcacggca
     3181 cgtacgcaga cttgttgacg gacgcgcagc ggctcgtgtc ggcgctgcgg gccgcgggca
     3241 ttgcgcccgg tgacgcggtt gcgttccaac ttccgaactg gcgggaagca gtcgtcgcgt
     3301 tctacgggct cgcgctcggc ggctacgtcc tcgtgccgat cgtgcacatc tacggcgcca
     3361 aggaggttcg gttcatcctc gcccagagcg gcgcccgcgc gtacatctcc gccgatcggt
     3421 acggcacggt cgactacgtc gacatcgtcg aaggtgccga cgcgggcgcg ctcgcgaagc
     3481 tcgacctcca tgtcgtcgtc ggcgacgcaa gcacgcccgg cgcgcacgtc acccgcatcg
     3541 cttgggacac ggtcgacgcg gcaccacccg cgagcgacgt cgttgcgtcg gatcccgacg
     3601 acgta
//
LOCUS       DASUGA010000012         2151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_132272, whole genome shotgun sequence.
ACCESSION   DASUGA010000012 DASUGA010000000
VERSION     DASUGA010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132272"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VFX21_00230"
     CDS             complement(<1..763)
                     /locus_tag="VFX21_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEX5094398.1"
                     /translation="MAAQQVDLLDRRLLFFTGKGGVGKSTVAAATALLAASVGRRVLL
                     VEVDAKGDIPAQFERAPVGFEPREIFPGVLAMAMDTEQSLQEYLRLNLRVPVLGRVGP
                     LARALDFVASAAPGVKEILTIGKICWEVRESIEGRADFDIVVVDAAATGHIVAQLGAP
                     EGIRDLVDVGPVRAQTQWLSEILEDPALTAVNVVTTPEEMPVAETIELVQRIRAETSV
                     PLGSVIVNRVLPELFTTADEETFAALTEPHVYERLR"
     gene            complement(781..1008)
                     /locus_tag="VFX21_00235"
     CDS             complement(781..1008)
                     /locus_tag="VFX21_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HEX5094399.1"
                     /translation="MEVRIGVVYSARELTVDTNESVDAVESAVEAALGGQKLFWLTDT
                     KGRRIGVPSDKIAYVEVAADAGERQVGFGTR"
     gene            complement(1048..>2151)
                     /locus_tag="VFX21_00240"
     CDS             complement(1048..>2151)
                     /locus_tag="VFX21_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094400.1"
                     /translation="VAGFPVLVVDPAKVAAVDPVSHHVTFTPTSNARDGGELIATPTL
                     ADITGDGHPEILVGAQEEYEDPPNIGSGADIVSLLSLAADVGNSRVYAISPQGTFVPG
                     WPVALGFLQLETLPSIGNGVSAQVAVADVAPQSPGPEVIAASAVGPLYVLNAAGDSVF
                     GQSGGKDIPLFWAAGLSGADAGHFGAFRNSTDIAASLVGFGGPSAGHLDADTTADVTA
                     PTAGLTRLFDVALPDQQMPNDDHLMAWRADTGDALVGFPQTTTDLAFFVTPAIADVDG
                     NGKRETIAGNGVYLLHAHEWDGRAPAGWPKLTGGWVVGTPGLGNWDGKGGAELAVVRR
                     DGVLLVWHTDAPASSLEWPRFGGNDRNSGTPSG"
BASE COUNT          328 a          775 c          724 g          324 t
ORIGIN      
        1 cacgcaatcg ttcgtacacg tgcggctcgg tcagtgccgc gaacgtctcc tcgtccgcgg
       61 tcgtgaagag ctcgggcagg acgcgattga cgatcacgct gccgagtggc accgacgtct
      121 cggcgcggat gcgctgcacg agctcgatcg tctccgcgac cggcatctcc tcgggtgtcg
      181 tcacgacgtt gaccgccgtg agcgcggggt cttcgaggat ctccgacagc cactgcgtct
      241 gcgcgcgcac gggcccgacg tcgacgaggt cccgaatgcc ctccggcgca ccgagctggg
      301 cgacgatatg accggtcgcg gccgcgtcga cgacgacgat gtcgaagtcg gcgcgccctt
      361 cgatcgactc gcgcacttcc cagcagatct tgccgatcgt gaggatctct ttcacgcccg
      421 gtgccgcgct ggcaacgaag tcgagggccc gcgccaacgg tccgacgcga ccgagcaccg
      481 gaacgcggag gttcaggcgc aggtactcct gcaacgattg ctcggtgtcc atcgccatcg
      541 cgaggacacc gggaaagatc tcgcggggct cgaagccgac cggcgcgcgc tcgaactgcg
      601 cggggatgtc gcccttggca tcgacttcga cgagcagcac gcgccggccg acgctggccg
      661 cgagcaacgc cgtcgcggcc gcaaccgtgc tcttgccgac gccgcccttc ccggtgaaga
      721 acagcaaccg ccggtcgagc agatcaacct gctgagcggc caagagcgct ttccctctcg
      781 ttacctcgtg ccgaagccga cctggcgctc gccggcatcc gccgcgacct cgacgtacgc
      841 gatcttgtcg ctcggcacac cgatgcgacg gcccttggtg tcggtgagcc agaacagctt
      901 ctggccgccg agcgcagctt cgaccgcgga ctcgaccgca tcgaccgact cgttcgtgtc
      961 gacggtgagc tcccgcgccg agtacacgac gccaatccgt acttccatct gtgcggcctc
     1021 cgctgcgttc gctgactggc cgggacccta cccggatggc gtaccgctgt tgcgatcgtt
     1081 gccgccgaag cgcggccatt cgagcgacga cgccggagca tcggtgtgcc acacgagcag
     1141 cacgccgtcc cggcggacga ccgcgagctc cgcacctccc ttgccgtccc agttcccgag
     1201 gcccggcgtg ccgacgaccc agccaccggt gagcttcggc caccccgcgg gcgcgcgccc
     1261 gtcccactcg tgcgcgtgca ggaggtacac gccgttcccc gcgatcgtct cgcgcttccc
     1321 gttgccgtcg acgtctgcga tcgcgggggt gacgaagaac gcgaggtccg tcgtcgtttg
     1381 cgggaagccg acgagcgcgt cgccggtgtc ggcgcgccag gccatcaagt ggtcgtcgtt
     1441 gggcatctgc tggtcgggca acgcgacgtc gaacaagcgc gtgagtcccg cagtcggtgc
     1501 cgtcacgtcc gcggtcgtgt ccgcatccag atgaccggcg gacggaccgc cgaacccgac
     1561 gagcgacgcc gcgatgtcgg tggagttgcg gaacgcgccg aagtgaccgg cgtccgcgcc
     1621 actgagaccg gccgcccaga acaaggggat gtccttgccg ccactctgtc cgaacacgct
     1681 gtcgccggcc gcgttcaaca cgtacagggg accaaccgcg ctcgccgcga tcacttcggg
     1741 tccgggcgac tgcggcgcaa cgtccgcgac tgcgacctgc gcgctgacgc cgttgccgat
     1801 cgacggcagc gtctcgagct gcaggaagcc gagggcaacg ggccatcccg gcacgaacgt
     1861 gccttgcggc gagatcgcgt acacacgcga gttgccgacg tcggccgcga gcgagagcag
     1921 gctgacgatg tcggcgcccg agccgatgtt cggcgggtct tcgtactcct cctgcgcgcc
     1981 cacgaggatc tcgggatgcc cgtcgccggt gatgtcggcg agcgtcggcg tcgcgatcag
     2041 ctcaccgccg tcgcgcgcgt tcgaggtcgg cgtgaacgtg acgtggtgcg acaccgggtc
     2101 gaccgccgcc accttggccg ggtcgacgac gagcaccggg aagcccgcga c
//
LOCUS       DASUGA010000013         2105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1801545, whole genome shotgun sequence.
ACCESSION   DASUGA010000013 DASUGA010000000
VERSION     DASUGA010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1801545"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            368..847
                     /locus_tag="VFX21_00245"
     CDS             368..847
                     /locus_tag="VFX21_00245"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5094401.1"
                     /translation="MTALGTLLERIGPGLFQQAYVVDDIAAAQAALQETLGCSPFVNL
                     PATDLDYEFRGGSVSCAMEIGFARSGNVQVELLQPVRGGGLHAEFLATNGPGAHHLGF
                     LVDDLDAIVAESGYERVMGAQFGSLTFCYLDTFEEFGLYTELVHDPDGMMMSLMPWR"
     gene            complement(831..1631)
                     /locus_tag="VFX21_00250"
     CDS             complement(831..1631)
                     /locus_tag="VFX21_00250"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX5094402.1"
                     /translation="MLTDTEIETFWRDGVVCVRGALPVELVSAMQAPVEAAIASAQSA
                     DLSALAGGTSGRFVAGVDHWRHQPELRSFACDSPLPALVASVLRSTAAYLYEDSVLVK
                     EPGAAERTHWHQDLGYFHVEGDQLCTTWCPLDAVDAESGAMQFVRGSHRWDRVFRPNL
                     FVTTEPLPGTAGEIVPEIDAARDDVIAFTLEPGDLTIHHARTLHAAGANRSADRRRRA
                     ISVRYCGDDARVKIRAGAPVKPEQRTLADGDTLGGPVHPQVWPVTATA"
     gene            complement(1631..>2105)
                     /locus_tag="VFX21_00255"
     CDS             complement(1631..>2105)
                     /locus_tag="VFX21_00255"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5094403.1"
                     /translation="GRSEYEPDVFEETVAINLFGAFRMASAVRSQLTASEFDGGASVV
                     NLGSMSSFFGIEIVPGYGAAKAGVVQMTKTLAVAWAKLGVRVNAVAPGVVETGMTAPM
                     LPFEQMTEPMLARTPLGRFAQPDEIAPSVLFLASPAARYITGHTVVVDGGFSIQG"
BASE COUNT          358 a          702 c          689 g          356 t
ORIGIN      
        1 ggttccgtcc gcgattgccg gcgttgcggt cacgacgtcg ggcgtcttgt agaaccacgc
       61 ttgctgcagg cggttgacgc tgccggtcgc gatgtcggtc ttgcagtcgt ccgggtacgc
      121 gaaggtgcgc gccggcgttc gaccgaacat cggccacgcg cagcgaagat cgccggtcga
      181 gagtgttgcg ctcttctccg acggcttgtc gctcccgcta cagcccgcaa ggacaccgaa
      241 gacaagcaac gcacagccga cgagccgtcg tctcgttccc cccattggcg gcgcagcata
      301 cgaatctctg acgctgccgt catatgtgcg cgaggatccc gcacacagga aagaggcaca
      361 gacgaacatg actgctctcg gcacactgct cgaacgcatc ggtcccggct tgttccagca
      421 ggcgtacgtc gtcgacgaca tcgcggccgc ccaagccgcg ctccaagaga cgctcgggtg
      481 ttcgccgttc gtcaacctgc ctgcgacgga tctcgactac gaattccgag gcgggagtgt
      541 ttcttgtgcg atggagatcg gattcgcgcg gagcggcaac gtccaggtcg agttgctcca
      601 acccgtgcgc ggcggcgggt tgcacgccga gttcttggcg acgaacggcc cgggcgcaca
      661 ccatctcggc tttctcgtcg acgacctcga cgcgatcgtc gcggaatccg gctacgaacg
      721 cgtcatgggc gcgcagttcg gatcgctgac cttctgttat ctcgacacgt tcgaagaatt
      781 cgggttgtac acggagctcg tgcacgatcc cgacggcatg atgatgagtc tcatgccgtg
      841 gcggtgaccg gccacacctg cgggtgtacc ggcccgccga gcgtgtcgcc gtcggcgagc
      901 gttcgttgtt ccggcttcac cggtgcgccg gcgcggatct tcacgcgcgc atcgtcaccg
      961 cagtagcgca ccgaaatcgc ccggcgccgc cgatcggcag atcggttcgc acccgccgca
     1021 tgcagcgtgc gggcgtgatg gatcgtcaga tcgcccggtt cgagcgtgaa cgcgatcacg
     1081 tcgtcgcgag cggcgtcgat ctccggcacg atctcgccgg ccgtgccggg cagcggctcc
     1141 gtcgtgacaa agaggttcgg acgaaacacg cggtcccagc gatgcgatcc gcgcacgaac
     1201 tgcatcgcgc ccgactccgc atcgactgcg tcgagcggac accacgtggt gcacagctgg
     1261 tcaccctcga cgtggaagta gccgaggtcc tgatgccagt gcgtgcgttc cgcggcaccc
     1321 ggttccttga cgagcacgct gtcctcgtag agatacgccg cagtgctgcg cagcaccgat
     1381 gcgaccagcg cgggtaacgg cgagtcacac gcaaacgaac ggagttcggg ttgatggcgc
     1441 caatgatcga cgcccgcgac gaaccggccg ctcgtaccac ccgccagcgc cgacaggtcg
     1501 gcgctttggg cgctcgcgat tgcggcttcg acgggcgctt gcatcgcgct gacgagttcg
     1561 acgggcagcg caccgcgcac gcacacgacg ccgtcgcgcc agaaggtctc gatctccgtg
     1621 tcggtgagca tcagccctgg atcgagaagc cgccgtcgac gacgacggtg tgaccggtga
     1681 tgtagcgcgc ggccggactg gcaaggaaga gcaccgacgg tgcgatctcg tcgggttgag
     1741 cgaagcgtcc gaggggcgtg cgagccagca tcggttcggt catctgttcg aacggcaaca
     1801 tcggcgcggt catcccggtt tcgacgacgc cgggcgcaac tgcgttcacg cgcacaccga
     1861 gcttggccca tgcgaccgca agcgtcttcg tcatctgtac gactccggct ttcgccgcgc
     1921 cgtagcccgg cacgatctcg atgccgaaga atgacgacat cgagccgagg ttgacgacgc
     1981 tggcgccacc gtcgaactcg ctcgcggtca gctgcgaccg aaccgcgctg gccatgcgaa
     2041 acgcaccgaa caggttgatc gcgaccgtct cctcgaagac gtcgggttcg tactcgctgc
     2101 gaccg
//
LOCUS       DASUGA010000014         2415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1938763, whole genome shotgun sequence.
ACCESSION   DASUGA010000014 DASUGA010000000
VERSION     DASUGA010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1938763"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VFX21_00260"
     CDS             <1..678
                     /locus_tag="VFX21_00260"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075165.1"
                     /note="catalyzes the reversible transfer of the terminal
                     phosphate of ATP to form a long chain polyphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEX5094404.1"
                     /translation="KTATTYEDLGVLSCDPDLGADLSELFNHLTGYSRQGEYRRLLVA
                     PMHLRDALRDLIRGQAHAGGRIAMKMNSLVDSEMIDELYAASRAGAQIELVVRSICCL
                     RPGVPGLSENIAVRSVVGRYLEHSRVFRFGEPGADETEYIIGSADLMPRNLDRRVEAM
                     LRVADPRMRARLDEVLTINVLYDALAWRLEPSGNWTKVDPTGGEESQVKFQDLALARA
                     KVRSSDA"
     gene            700..1566
                     /locus_tag="VFX21_00265"
     CDS             700..1566
                     /locus_tag="VFX21_00265"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX5094405.1"
                     /translation="MSTRAVTDVVQQAIASAVERLLENEAGVRAGNDDEAVHQARVAT
                     RRLRSDLRTFEFVLDDAAVRDLRGELRWLGSELGVVRDLDVLRARLREHATKLPAAES
                     EAAQRVVRRLDADRHAAGTELHSYLEQSRYRQLRDALRDAAAQPPFRSDATKAGVDEL
                     HNAVDRRWKKLRRGVEDLGDTPADDALHHVRVLAKRARYAAEASVPMFGKPAKKLAAA
                     VEAIQESLGEHHDAVVAQAWLAKTAHECTPAEAYAVGMLAQVEREAADDARGAFEDAW
                     RAARKTHKRVWT"
     gene            1520..1987
                     /locus_tag="VFX21_00270"
     CDS             1520..1987
                     /locus_tag="VFX21_00270"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5094406.1"
                     /translation="MRGERRARRTSASGPEVTPVVRAAGGVVTRRAADGTLEVLAVHR
                     PRYGDWSLPKGKCDPGETDEDAAVREVAEETGVVCTLGAELESMHYVDRRGRPKVVRY
                     WEMTPAGDAPWEPNDEVDEIAWLPAYRAAAVLSYDGDRRLVAAVVDQSGGGPG"
     gene            1984..>2415
                     /locus_tag="VFX21_00275"
     CDS             1984..>2415
                     /locus_tag="VFX21_00275"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HEX5094407.1"
                     /translation="MTIYLVRHAKAGERGGWDGEDWLRPLSARGHLQTRGLLELLRDK
                     KFERVVSSPYVRCMETVVPLAGVRGLAIEPSDALAEGATLEEAVALVRKHASHGAVFS
                     THGDVMPMLLEHYSLQGVDIGPTPQWPKGCTWVLETDQTGDV"
BASE COUNT          410 a          807 c          847 g          351 t
ORIGIN      
        1 aagacggcca cgacgtatga ggaccttgga gtgctgtcgt gcgatcccga tctcggcgcc
       61 gacctctccg agctcttcaa ccacttgacc ggctacagcc ggcagggcga gtaccggcgg
      121 ctgctcgtcg caccgatgca cttgcgcgac gcgctgcgcg acctgattcg cggccaggct
      181 cacgccggcg gccgcatcgc gatgaagatg aattcgcttg tcgactccga gatgatcgac
      241 gagctctacg ccgcgtcgcg tgccggagcg cagatcgaac tcgtggtgcg cagcatttgc
      301 tgcctgcggc cgggcgtccc cggactgtcg gagaacatcg ccgttcggtc ggtcgtcggc
      361 cggtacctcg agcactcgcg cgtgttccgc ttcggcgaac ccggtgccga cgaaaccgaa
      421 tacatcattg gctccgccga cctgatgccg cgcaacctcg accgccgcgt cgaagccatg
      481 ctgcgcgtcg cggatccccg catgcgcgcc cggctcgacg aagtcctcac gatcaacgtg
      541 ctgtacgacg cactggcgtg gcggctcgaa ccgagcggca actggacaaa ggtcgacccg
      601 accggcggcg aggaatcgca agtgaagttc caagatctgg cgttggcgcg cgccaaggtg
      661 cgcagctccg atgcctgact cgcggatgtc gagttcgtca tgagcacgcg cgcggtcacc
      721 gacgtcgtgc agcaggcgat cgcctcggcg gtcgaacggt tgctcgaaaa cgaagccggg
      781 gtacgggccg gcaacgacga cgaagcggtg caccaagcgc gcgtcgcgac ccggcgtctc
      841 cgttccgacc tgcgcacctt tgaattcgtg ctcgacgacg cggcagttcg cgacctgcgc
      901 ggcgaactgc gatggctcgg atcggaactc ggcgtcgtgc gcgatctcga cgtcctacgc
      961 gcacgcctgc gcgagcacgc cacgaagctt ccggccgccg aatcggaagc cgcgcagcgg
     1021 gtcgtccgcc ggctcgacgc cgaccgccac gccgccggga ctgaactgca tagctatctt
     1081 gaacagtctc gttaccggca gttgcgcgac gcgctgcgcg acgcggccgc ccaaccgccg
     1141 ttccgttcgg acgcgacgaa agccggcgtc gacgaactcc acaacgcggt cgaccgccgg
     1201 tggaagaagc tgcggcgcgg cgtcgaagat ctcggcgaca cgcccgccga cgacgcgttg
     1261 caccacgtgc gcgtgctcgc gaagcgcgcc cgctacgcgg ccgaggcgtc cgtgccgatg
     1321 ttcggcaaac ccgccaagaa gctggccgcg gcggtcgaag caatccaaga gtcgctcggc
     1381 gagcaccacg acgccgtcgt ggcccaagcc tggctggcga agaccgcaca cgagtgcacg
     1441 cccgccgagg cgtacgcagt cggcatgctg gcgcaagtcg agcgcgaagc cgccgacgac
     1501 gcacgcggcg cgttcgaaga tgcgtggcga gcggcgcgca agacgcacaa gcgcgtctgg
     1561 acctgaggtg acgccagtcg tccgagcggc gggcggagtg gtgacgcgac gggcggccga
     1621 cggcacactc gaagtgctcg cggtgcaccg gccccggtac ggcgactggt cgctgcccaa
     1681 gggcaagtgc gatccgggcg agacggacga ggacgccgcc gtacgcgagg tcgccgaaga
     1741 gaccggcgtc gtgtgcaccc tgggggccga gctggagtcg atgcactatg tcgaccggcg
     1801 cgggcggccc aaggtggttc gttactggga gatgacaccg gcgggtgacg cgccctggga
     1861 accgaacgac gaggtcgacg aaattgcctg gctaccggca taccgcgcgg ctgcggtgct
     1921 ctcctacgat ggcgaccgtc ggttggttgc ggcggtcgtg gaccagtccg gcggaggacc
     1981 cgggtgacga tctatctcgt ccgccatgcg aaggcgggag aacgcggcgg ttgggacggc
     2041 gaggattggc tgcgcccgct ttccgctcgc ggtcacctgc aaacgcgggg tctgttggag
     2101 ctgctgcgcg acaagaagtt cgaacgtgtc gtctcgagtc cgtacgtgcg ttgcatggaa
     2161 accgtggtac cgctcgccgg cgtgcggggt ctcgccatcg agccgagcga cgcgctcgcc
     2221 gaaggcgcga cactcgagga agccgtcgca ttggttcgca agcacgcgtc gcacggcgcc
     2281 gtgttctcga cgcacggcga cgtgatgccg atgctgctcg agcactactc gttgcaaggc
     2341 gtcgacatcg gtcccacgcc gcaatggccc aaaggctgca cgtgggtgct ggagacggac
     2401 caaacgggcg acgtg
//
LOCUS       DASUGA010000015         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_967561, whole genome shotgun sequence.
ACCESSION   DASUGA010000015 DASUGA010000000
VERSION     DASUGA010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967561"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="VFX21_00280"
     CDS             complement(<1..1142)
                     /locus_tag="VFX21_00280"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEX5094408.1"
                     /translation="METEAGIVLVTTSPDWETRVRKAFDGRLNGELSQRPEACTPGEP
                     QTALDVVLAGGPRVVVLGPDCDVDHSLAVAREIDDRHHETCTVLVADPTPELWEKALR
                     AGVRDIVAPDAALVDVRNVIERAVETAAKRRAVASTDAAQREAKILAVVSPKGGAGKT
                     AIATNLAVGLAHEHPGDVVLVDLDLQFGDVANALRLDPETTITDVSRAVGAIDATTLK
                     AYLTPHSSGLFVLCAPETPGVADDIAIEHVGTAIELLSQVFRYVVVDTAAGLDDVALT
                     AMERASDLVVVCGTDVASARGARKELEAFNMLGLTTQRRIFVLNRADARVGLNVRDIE
                     STVGMSVDFSIPSSRAVPLSMNQGTPLLDMDARTPVSKAFSDLVST"
     gene            complement(1142..1906)
                     /locus_tag="VFX21_00285"
     CDS             complement(1142..1906)
                     /locus_tag="VFX21_00285"
                     /inference="COORDINATES: protein motif:HMM:NF028286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcpC/CpaB family pilus assembly protein"
                     /protein_id="HEX5094409.1"
                     /translation="MAAVALAAIGTVVIVGYVNGAEDRALAGEEVVKVLVVTDQIPAG
                     TPAEDIGDRVKIEQVVEKVKADGAVIDVDDLKGKVASATLVPGEQVIEKRFVEATAYR
                     AGTNVAVPDGMLQTTVSLSPDRAVGGVLSPGTKVAVSMSFEATKTAQNADGATSGDTS
                     DQLTHMTLRKVLVTRVQIDESAAADTPDDSADEANLTKPADAPTSNLLITLAIDDVNT
                     ERLVWAAEHGTLWLSVEEDDTPVGPTKIVTEENVLS"
     gene            complement(1957..>2411)
                     /locus_tag="VFX21_00290"
     CDS             complement(1957..>2411)
                     /locus_tag="VFX21_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094410.1"
                     /translation="NNGPGNWGVMDFDGGANSNADTKEWTRNGYPGEITLPSTVPGDT
                     GAFSNSLGSELTYLENSGEFFGLPIYDSAAGNGSNAKFHVIAVAYVKLIDFKANGAEA
                     QRYMTLVFDRGVMEGRCCNPNGIDTGVRALRICDVDTLNPNTSDPRAC"
BASE COUNT          337 a          816 c          824 g          434 t
ORIGIN      
        1 atcgtgctga ccaagtcact gaacgccttg ctcaccggcg tccgggcatc catgtcgagc
       61 agcggcgtgc cctggttcat cgacagcggg accgcgcgcg agctcgggat cgagaagtcg
      121 acactcatgc cgacggtcga ctcgatgtcg cgcacgttga gcccgacgcg ggcgtcggcg
      181 cggttcaaca cgaagatccg ccgttgcgtc gtcaacccga gcatgttgaa cgcctcgagc
      241 tccttccggg cgccgcgcgc gctggcgacg tcggtgccgc agacgacgac gaggtcgctg
      301 gcgcgttcca tcgccgtcag tgcgacgtcg tcgaggccgg ccgcggtgtc gacgacgacg
      361 taacggaaca cctgcgacag aagctcgatg gccgtcccga cgtgctcgat cgcgatgtcg
      421 tcggcgacac ccggggtctc gggtgcgcag agcacgaaga ggcccgaaga gtgcggcgtc
      481 aggtacgcct tcagcgtcgt cgcgtcgatg gcgccgacgg cgcgggagac gtcggtgatc
      541 gtcgtttccg gatccagccg caatgcgttc gcgacgtcgc cgaactggag gtcgaggtcg
      601 acgagcacga cgtcgccagg gtgctcgtgc gccaggccca ccgccaagtt cgtggcgatc
      661 gccgtcttgc ccgcgccacc cttcggcgag acgacggcga ggatcttggc ttcgcgctgt
      721 gcggcatccg tcgaggccac ggctcgccgc ttcgccgcgg tctcgaccgc ccgctcgatc
      781 acgttgcgga cgtcgacgag tgcggcgtcc ggcgccacga tgtcgcgcac gccggcgcgc
      841 agcgccttct cccagagctc gggcgtcggg tcggcgacca acaccgtgca cgtctcgtgg
      901 tgccggtcgt cgatctcgcg ggcgacggcc agcgagtggt cgacgtcgca gtcgggcccc
      961 agcacgacga cgcgggggcc gccggcgagg accacgtcca gtgcggtctg tggctcgccg
     1021 ggcgtgcacg cctccgggcg ctgcgacagc tcgccgttga ggcggccgtc gaacgccttg
     1081 cggacgcgcg tctcccagtc ggggctcgtc gtcaccagga cgatgccggc ttcggtctcc
     1141 atcacgacag cacgttctct tcggtcacga tcttcgtcgg gccgaccggc gtgtcgtctt
     1201 cctcgaccga cagccacagc gtgccgtgct cggccgccca caccagtcgc tcggtgttga
     1261 cgtcgtcgat tgccaaggtg atgagcaggt tgctggtcgg cgcgtcggcc ggtttcgtca
     1321 ggttcgcctc gtcggcgctg tcgtcgggcg tgtcggcggc cgcggactcg tcgatctgca
     1381 cgcgggtgac gagcaccttg cgcaatgtca tgtgggtcag ctggtcgctc gtgtcgccgg
     1441 acgtcgcgcc gtcggcgttc tgcgccgtct tcgttgcctc gaacgacatg gagaccgcga
     1501 ctttcgtgcc gggggacagc acgccgccga ccgcgcggtc aggggagagc gacacggtcg
     1561 tctgcagcat gccgtcgggc accgccacgt tggtgccggc tcggtacgcg gtcgcctcga
     1621 cgaagcgctt ctcgatcacc tgctcgccgg gcacgagcgt tgcgctcgcg accttcccct
     1681 tgaggtcgtc gacgtcgatg accgcgccgt cggccttgac cttctcgacg acctgctcaa
     1741 tcttgacgcg gtcgccgatg tcctcggccg gagtcccggc cgggatctga tcggtcacga
     1801 ccagcacctt cacgacttcc tcgcctgcca gcgcgcgatc ttccgcgccg ttgacgtaac
     1861 caacgatcac gacggtaccg atcgctgcca gagcgacggc tgccatgagg cccaccactt
     1921 tgcgattcac agtgtcactc ctgaactttg tttgagctaa caggctcggg gatctgaggt
     1981 gttcggattc aaggtgtcga cgtcgcagat gcgcagtgca cgcacgccgg tgtcgatgcc
     2041 gttcgggttg cagcagcggc cttccatgac gcctcggtcg aagacgaggg tcatgtagcg
     2101 ctgtgcttcg gcgccgttgg ccttgaagtc gatgagcttc acgtaggcga cggcgataac
     2161 gtggaacttc gcgttcgacc cgttgccggc cgcgctgtcg tagatcggca ggccgaagaa
     2221 ctcaccggag ttctccaggt acgtcagctc cgacccgagc gagttgctga acgcacccgt
     2281 gtcgccgggc acggtcgacg ggagcgtgat ctcgccgggg tagccgttgc gcgtccactc
     2341 cttggtgtcg gcgttgctgt tggcgccgcc gtcgaagtcc atgacgcccc agttgccggg
     2401 accgttgttg c
//
LOCUS       DASUGA010000016         2611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1662694, whole genome shotgun sequence.
ACCESSION   DASUGA010000016 DASUGA010000000
VERSION     DASUGA010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1662694"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..583
                     /locus_tag="VFX21_00295"
     CDS             188..583
                     /locus_tag="VFX21_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094411.1"
                     /translation="MSLPEHPNAPRRTALVLAFAGIVVAGTLGGLIGWGIVDTTCSET
                     PTIAEQLLGQVPGHEVQHQECGAKLLGGALAGAVIAAAGAGVVAVLMLRAQSEWKAHP
                     PQPLTAAQRAQLARSKGRSGPGSLDRDRK"
     gene            complement(613..1320)
                     /locus_tag="VFX21_00300"
     CDS             complement(613..1320)
                     /locus_tag="VFX21_00300"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEX5094412.1"
                     /translation="MSEAPARVLAIYAHPDDPEISAGGTLARWAAAGSEVHVLITTRG
                     DKGSSDPNTDTDELAQIRKRESLAAARVLGVAGVLHLDHPDGDLPDDRTLRGELVRHV
                     RLYRPEVVLCPDPTAVFFGEGYINHRDHRVTGWAAIDAVAPAAGNPHYFPELRGEGLD
                     VHSPSAIYLSGTHEPNVWVDIAASLDRKIEALLCHASQLTETGEWFNEFLRESAEAAG
                     RAAGVTYAEGFRRIVLA"
     gene            complement(1323..2201)
                     /gene="tesB"
                     /locus_tag="VFX21_00305"
     CDS             complement(1323..2201)
                     /gene="tesB"
                     /locus_tag="VFX21_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HEX5094413.1"
                     /translation="MGSALDELIELLQLEQLEVNLFRGLSPDENRQRVFGGQVAGQAL
                     VAAGRTVDIGPVHSLHAYFLRPGDPRVPIVYEVDRIRDGRSFATRRVVAIQHGRAIFN
                     LSASFHAVEPGPEHQDQMPDAPDPESLPTLVERIAGHEDKFPEPMLEWLRRERPVETR
                     STRLPRYLDPEPGVRKPEQLVWFRANGSLPDDPMLHDCIIAYASDLTLLDTAVMAHAN
                     SWDDDRFMIASLDHAMWFHRNVRADEWLLYHQKSPSAQNARGLAEGYIFSRDGRLAVT
                     VMQEGLIRPVKPGWDQ"
     gene            complement(2230..>2611)
                     /locus_tag="VFX21_00310"
     CDS             complement(2230..>2611)
                     /locus_tag="VFX21_00310"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020115266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX5094414.1"
                     /translation="IVVAAEGATPAAGTMELQSGELDDFGHVRLGGIGQQVADEIERR
                     TGFEARVTALGHVLRGGSPTAYDRVIATRFGVEAIDAVHEGDFGKMVALRGTDVVRVP
                     IEEGVEQLKTVDAKLFDTAAVFFG"
BASE COUNT          396 a          923 c          889 g          403 t
ORIGIN      
        1 ggcgagcaac gagtcgtctt cggcgtcgat cacaccgccc ggcgtgctcc agtcctcgaa
       61 cccgccacgg cgcacgttac gcacgagcaa cacgccctcg ggcgtctcca acagggctcc
      121 ggcgacggtc cattcgcgca tgaccggatc agtctcgcgc agcaggtcgt cggtacgatc
      181 gcccctcgtg agcctccccg aacacccgaa tgccccccgc cggacggcgc tggtgctggc
      241 gttcgcgggc atcgtggtcg cgggcacact gggcggcctc atcggctggg gcatcgtcga
      301 cacgacgtgc agcgagacgc cgacgatcgc cgagcaactc ctcggccagg tgccggggca
      361 cgaggtgcag caccaggagt gcggcgcgaa gttgctcggc ggcgcgctcg ccggtgcggt
      421 catcgccgcg gccggcgccg gcgtcgtcgc ggtgttgatg ttgcgcgcgc agtcggagtg
      481 gaaggcgcac ccgccgcagc cgctcaccgc cgcccaacgc gcccagcttg cccgctcgaa
      541 gggccgctcg ggccccggct cgctggatcg cgatcgcaaa tagatgcggg cgctccgctc
      601 gcctcgggct cgctaggcga gcacgatccg gcggaacccc tcggcgtacg tgaccccggc
      661 cgcgcggccc gcggcctcgg cgctctcgcg caagaactcg ttgaaccact cgcccgtctc
      721 ggtgagctgg ctcgcgtgac acagcaacgc ttcgatcttg cggtcgagcg acgccgcgat
      781 gtcgacccag acgttgggtt cgtgcgtgcc cgagagatag atcgcgctcg gcgagtgcac
      841 gtcgagtccc tcgccgcgca gctcggggaa gtaatgcgga ttgcccgccg cgggcgcgac
      901 cgcgtcgatg gcagcccaac cggtgacgcg atggtcgcga tggttgatgt agccctcacc
      961 aaagaacacc gcggtcgggt cggggcagag gacgacttcg gggcgataca accgcacatg
     1021 acggaccaac tcgccccgca aggtgcgatc gtccggcagg tcgccgtcgg ggtggtcgag
     1081 gtgcaggaca cccgcgacac cgagcactcg cgccgcggcc agactctcgc gtttgcggat
     1141 ctgtgcgagc tcgtcggtgt cggtgttcgg gtcgctgctg cccttgtcgc cgcgcgtcgt
     1201 gatgaggacg tgcacttccg acccggccgc ggcccaccgt gccagcgtgc cgcccgcgga
     1261 gatctcggga tcgtcgggat gcgcgtagat cgccaggacg cgcgcgggcg cctccgacac
     1321 gatcattgat cccagcccgg cttcaccggc cggatcaatc cctcttgcat caccgtcacc
     1381 gcgagccggc cgtcacggct gaagatgtac ccctcggcga gcccgcgcgc gttctgggcc
     1441 gacgggctct tctggtggta cagcagccac tcgtcggcgc gcacgttgcg gtggaaccac
     1501 atcgcatggt cgaggctcgc gatcatgaac cggtcgtcgt cccacgagtt cgcgtgcgcc
     1561 atcaccgcgg tgtccagcaa cgtgaggtcg gacgcgtacg caatgatgca gtcgtgcaac
     1621 atcgggtcgt cgggcaacga gccgttggcc cggaaccaca cgagttgctc gggcttgcgg
     1681 acacctggtt cgggatcgag ataccgcggc aggcgcgtcg aacgcgtctc gaccgggcgt
     1741 tcgcgccgta gccactcgag catcggctcg ggaaacttgt cttcgtggcc ggcgatgcgc
     1801 tcgacgagcg tcggcagtga ctcggggtcg ggcgcgtcgg gcatctgatc ctgatgctcg
     1861 ggccccggct cgaccgcgtg gaacgacgcc gacaggttga agatcgcccg gccgtgctgg
     1921 atggccacga cccgtcgcgt cgcaaagctg cgaccgtcgc gaatgcggtc gacctcgtag
     1981 acgatcggca cacgcggatc tcccggccgc aagaagtacg cgtgcagcga gtgcacggga
     2041 ccgatgtcga ccgtgcggcc cgccgcgacc agcgcctggc ccgcgacctg accgccgaag
     2101 acgcgctggc ggttctcgtc ggggctgagc ccgcggaaca ggttgacctc gagctgttcg
     2161 agttgcagca gttcgatcag ctcgtcgagc gcggacccca ttgtggggcg attctacaaa
     2221 gccgcgtgcc tagccgaaga agacggccgc cgtgtcgaag agctttgcat cgaccgtctt
     2281 gagctgctcg acgccttctt cgatcggcac ccgcacgaca tccgtgccgc gcaaggcgac
     2341 catcttgccg aagtcgcctt cgtgcaccgc gtcgatcgct tcgacaccga agcgcgtcgc
     2401 aatgacgcgg tcgtaggcgg tcgggctgcc gccgcgcagc acgtggccga gcgcagtcac
     2461 gcgcgcttcg aagcccgtgc gccgctcgat ctcgtccgcg acctgctgac cgatgccacc
     2521 cagtcgcacg tgaccgaagt cgtcgagctc acccgactgc aactccatcg tgcccgcggc
     2581 cggcgtcgcg ccttccgcgg cgacgacgat c
//
LOCUS       DASUGA010000017         5133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1243110, whole genome shotgun sequence.
ACCESSION   DASUGA010000017 DASUGA010000000
VERSION     DASUGA010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243110"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VFX21_00315"
     CDS             complement(<1..780)
                     /locus_tag="VFX21_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5094415.1"
                     /translation="MQASQSSPLASPLSSPLIQIDGLTKRYPGVTALDNLSVEVPRGR
                     IGLVGANGAGKTTTFRLLLGLAHPTEGRIEVCGVDVARDPIGVRSRLGYMPEHDCLPL
                     DQTAADVVSTFGEMSGLPARAARQRASDILDLVGLDEARFRPIGGFSTGMRQRAKLAQ
                     ALVGDPELVLLDEPTAGLDPLGREDMLALVARLGSFGISVLMATHLLDDVQKVCDHVV
                     MIDGGKLVVAGATDSLLERTGTFTVDVGPHHATLVAGLLAAN"
     gene            809..1711
                     /locus_tag="VFX21_00320"
     CDS             809..1711
                     /locus_tag="VFX21_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017974721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEX5094416.1"
                     /translation="MDAWPDLTLSEWEDTRDTLHLWTQIVGKCRLALEPMVNQWWQVT
                     LYVSARGLTTSLMHAPGRDLEMEFDFLDHDLVIKTADGERRTIALAPRTVADFYEQTM
                     RTLDELGVRVSFLRRPVEVEVSVPFAEDTVHHSYDPDAAHRFWVALRHCHRVMYAFRG
                     RYVGKVSPVHYFWGGADLAVTRFSGRTAPKHPGGVPNCADFVQELAYSHEVSSAGYWP
                     GGGGGGVFYSYAYPTPAGFRDRAVVPADASYDDALGEFILPYDVVRAASDPDAVLMSF
                     LQSTYEAAAELASWPRADLEVRIT"
     gene            complement(1704..3062)
                     /locus_tag="VFX21_00325"
     CDS             complement(1704..3062)
                     /locus_tag="VFX21_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HEX5094417.1"
                     /translation="MTGLLRWFDQRLRITHFTRSALNKIFPDNWSFMLGEIALYCFIV
                     LVLTGTYLTFFFNPSDREVVYDGSYAPLRGVTMSEAYESTLRLSFDVRAGLVFRQMHH
                     WAALIFVGAVVVHLCRIFFTGAFRKPRDVNWVVGVTLLILVIFNGFTGYSLADDLLSG
                     TGLRIGYSIALAVPLVGTWAAFLVFGGEFPAPGLIPRLFVMHILLVPALIVALLSVHL
                     GIVWRQKHSQFPGPGRTEHNIVGTRLWPTYAARSIGLFFGVSAVVAALGGLVQINPIW
                     LYGPFHPAAVTTASQPDWYMGWLEGALRLAPAWRIHVFGFTVSELFWPGVLLPGITFG
                     LLYAWPFIEARATRDREEHNILDRPRLRPMRTAIGVGVLTFYIVLFVAGSQDIVAQRL
                     TASIPTVLWTLRVALVVLPPLCALLAYRLARDLHRSEDLEEEKDRIVEQAHVERVLDR
                     SM"
     gene            complement(3059..3646)
                     /locus_tag="VFX21_00330"
     CDS             complement(3059..3646)
                     /locus_tag="VFX21_00330"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX5094418.1"
                     /translation="MSERRVLPLAAGLLVALGAVSGPIEIGAAANLGLHMVQHVLLLT
                     VAAPLLAFGVSWGRDASARVARTALVVGFAAQTIVMVGWHVPVVYDAANEHVPLHVFE
                     HVTLLLGAGLFWWAVVHLPRPDLGFAVLALFASTLPCTAVGAGLALAPEPWYGAYPSL
                     SQQQMAGVLMWIVPGALFTVAGAAAFAAWITAAER"
     gene            complement(3639..4037)
                     /locus_tag="VFX21_00335"
     CDS             complement(3639..4037)
                     /locus_tag="VFX21_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094419.1"
                     /translation="MTMHTTAEAARRSHSIWSAVRNAFEVWYALLGSIAAWTIHILVL
                     ASVVQYTCNVDGALWIMHLTTVVTLAMAGAAMVLSWRLARHGRDDETPDEAGRNRFLG
                     VLGLLIGAANVALILLEEVYVLAFHSVRCV"
     gene            complement(4034..4627)
                     /locus_tag="VFX21_00340"
     CDS             complement(4034..4627)
                     /locus_tag="VFX21_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX5094420.1"
                     /translation="MTATGTTVGPSTRRGYSTAWWGMAVLIMTEAMVFAILIASYFFL
                     RAAADTWPPPGIDPPELALAFPFSFVLWGSSIPLFWAEANLRNGRVDRFRIGMLISFL
                     MGLAFLGYTFYDFHHLDFGWRDNAYGSAFYTIVGLHAAHVVVGLGMSIVVQIKAALGR
                     YDRGAHASAEVFALYWHFVDGVWLLVFPSLFLAAHIR"
     gene            complement(4624..>5133)
                     /locus_tag="VFX21_00345"
     CDS             complement(4624..>5133)
                     /locus_tag="VFX21_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX5094421.1"
                     /translation="WDGLNLMVSIGSAIFGLGTAITLWNFVHSRLRGAPAPANPWDGD
                     SLEWSVPSPPPHYNFAAIPIVESRHPLWQDTPLRYAQSGTEPETESLGAEGAVRRETP
                     VTSGIDTMVEGNLRIPRETYLPVAAAFGVMLFFFGLLANATLVAIVGAILALVGVLWW
                     TWRTEEDLI"
BASE COUNT          938 a         1692 c         1795 g          708 t
ORIGIN      
        1 attcgcggcg agcagtccgg ccacgagagt tgcgtggtgc gggccgacgt cgacggtgaa
       61 ggtgccggtg cgttcgagca gcgaatcggt cgcaccggcg acgacgagct tcccgccgtc
      121 gatcatcacg acgtggtcgc acaccttctg cacgtcgtcg agcaggtgcg tcgccatcaa
      181 gaccgagatg ccgaacgagc cgaggcgcgc gacgagcgcc agcatgtctt cgcgaccgag
      241 cgggtcgagg ccggccgtcg gttcgtcgag gagcaccagc tcggggtcgc cgacgagggc
      301 ctgcgcgagc ttggcccgct ggcgcatgcc ggtcgagaac ccaccgatcg gccggaagcg
      361 cgcttcgtcg agcccgacca ggtccaagat gtcggacgcg cgctggcggg ccgcacgcgc
      421 cgggagcccg ctcatctcgc cgaacgtgga cacgacgtct gccgcggtct ggtcgagcgg
      481 gaggcagtcg tgctcgggca tgtagccgag ccgcgaccgc acaccgatcg gatcgcgggc
      541 gacgtcgacg ccgcacactt cgatgcgtcc ttcggtcggg tgcgcgagcc cgagcaacag
      601 tcggaacgtg gtcgtcttcc ccgcaccatt tgcgccgacg aggccgatgc gaccgcgcgg
      661 cacctcgacc gagaggttgt ccagcgcggt gacgcccgga taccgcttcg tgagcccgtc
      721 gatctgtatc aggggagatg acagaggaga cgcgagtgga gacgattggg aagcctgcac
      781 gattggtccg agcctaggct gcgcggccgt ggatgcgtgg cccgacctca cgctgtccga
      841 gtgggaagac acccgcgaca cgctgcatct ctggacacag atcgtcggca agtgccgcct
      901 cgcgctcgaa ccgatggtga accagtggtg gcaggtcacg ctctacgtgt cggcgcgcgg
      961 gctcacgacg tcgctgatgc acgcaccggg tcgcgacctc gagatggaat tcgactttct
     1021 cgaccacgac ctggtgatca agaccgccga cggcgagcgc cgcacgatcg cactcgcgcc
     1081 gcgcaccgtt gccgacttct acgagcagac gatgcgcacg ctcgacgaac tgggcgtgcg
     1141 cgtgtcgttc ctgcgccggc cggtagaggt cgaggtgtcg gtgccgtttg ccgaggacac
     1201 ggtgcatcat tcgtacgatc cagacgcggc acaccgcttc tgggttgcat tgcgccactg
     1261 ccatcgcgtc atgtacgcct tccgcggccg ctacgtcggc aaggtcagcc ccgtgcacta
     1321 cttctggggt ggcgccgatc tcgcagtcac gcgcttctcc ggccgcaccg cgcccaagca
     1381 tcccggtggc gtgccgaact gtgccgactt cgtgcaggag ctggcgtaca gccacgaggt
     1441 atcgagcgcg ggctactggc ccggcggagg tggtggcggc gtgttctact cgtacgcgta
     1501 cccgacgccc gccggtttcc gcgaccgggc cgtcgtgccc gcggacgcgt cctacgacga
     1561 cgcgctcgga gaattcatcc ttccgtatga cgtcgttcgc gccgcgagcg accccgatgc
     1621 ggtgctgatg tcgttcttgc agagcacgta cgaagcggcg gccgagctcg cgtcgtggcc
     1681 gcgcgccgac ctcgaggtgc gcatcacata gaccgatcga gcacgcgctc gacgtgcgcc
     1741 tgttcgacga tccggtcctt ctcttcctcg agatcttcgc tgcggtgcaa gtcgcgtgcg
     1801 agccgatagg cgaggagcgc gcatagtggc ggcaggacga cgagcgcgac gcgcaacgtc
     1861 cacagcacgg tcgggatcga agcggtgagc cgttgggcga cgatgtcctg cgagccggcg
     1921 acgaagagca cgatgtagaa ggtcaacacg cccacgccga tcgccgtgcg catcgggcgc
     1981 aggcgcgggc gatcgaggat gttgtgctcc tcgcgatctc tcgtggcgcg cgcttcgatg
     2041 aacggccagg catagagcag gccgaacgtg atgccgggca gcagcacacc gggccagaac
     2101 agctccgaga cggtgaagcc gaagacgtgg atgcgccacg cgggcgcgag ccggagtgca
     2161 ccttcgagcc accccatgta ccagtccggt tgtgatgcgg tcgtgaccgc ggcggggtgg
     2221 aaaggtccgt acagccagat ggggttgatc tgcacgagtc cgccgagtgc ggcgacgacc
     2281 gcggagacgc cgaagaacag cccgatcgaa cgggccgcgt acgtcggcca cagacgggtg
     2341 ccgacgatgt tgtgctccgt gcggcccggc ccggggaact ggctgtgctt ctgccgccac
     2401 acgatgccga ggtgcaccga caacaacgcg acgatcaatg cgggcacgag caggatgtgc
     2461 atcacgaaca ggcgcgggat caagcccggc gcggggaact cgccgccgaa gacgaggaac
     2521 gcggcccatg tgccgacgag cggcaccgcg agcgcgatcg agtagccgat gcggagcccc
     2581 gtacccgaga gcaagtcgtc ggccagcgag tatcccgtga acccgttgaa gatgacgagg
     2641 atgagcagcg tgacgccgac gacccagttg acgtcgcgcg gcttgcggaa cgcgccggtg
     2701 aagaagatcc ggcacaagtg cacgactact gcgccgacga agatgagcgc ggcccagtgg
     2761 tgcatctggc ggaacacgag gccggcccgg acgtcgaagc tcagccgcag ggtcgactca
     2821 taggcctccg acatcgtcac gccgcgcagc ggcgcgtagc tcccgtcgta cacgacttcg
     2881 cggtcgctcg ggttgaagaa gaacgtgaga tacgtgccgg tcagcacgag gacgatgaag
     2941 cagtacaacg cgatctcgcc caacatgaac gaccagttgt cggggaagat cttgttcaac
     3001 gccgagcgcg tgaagtgggt gatgcgcaac cgctggtcga accaacgcag caggccggtc
     3061 atcgttcggc cgccgtgatc catgccgcaa aggccgcggc gccggcgacc gtgaacagcg
     3121 cgccggggac gatccacatg agcacgcccg ccatctgctg ctggctcagc gacgggtacg
     3181 cgccgtacca cggctcgggc gcgagggcga ggcccgcacc gactgccgtg cagggaagtg
     3241 tcgacgcgaa cagtgcgagg acggcgaacc ccaagtccgg acgcgggagg tgcacgacgg
     3301 cccaccagaa gagccccgcg cccaggagca gcgtgacgtg ctcgaagacg tgcagcggca
     3361 cgtgctcgtt cgccgcgtcg tagacgaccg gaacatgcca cccgaccatc acgatcgtct
     3421 gcgcggcgaa ccccaccacg agcgcggtgc gggcgacccg cgccgacgca tcgcggcccc
     3481 acgagacgcc gaaggccagc aacggcgcgg ccaccgtcag caacaacacg tgctggacca
     3541 tgtgcaggcc gaggttcgcg gccgcgccga tctcgatcgg tccggagacc gcgccgagcg
     3601 ccaccagcag gccggccgcg agcgggagca cgcgtcgctc agacacagcg caccgaatgg
     3661 aacgcgagga cgtacacctc ttcgagcagg atcagtgcga cgttcgcggc gccgatgagg
     3721 aggccgagca cgccgaggaa gcggttccgg cccgcctcgt cgggagtttc gtcgtcgcgg
     3781 ccgtggcggg cgagccgcca ggaaagcacc atcgcggcgc cggccatcgc gagggtgacc
     3841 acggtcgtga ggtgcatgat ccacagggcg ccgtcgacgt tgcacgtgta ctgcacgacg
     3901 ctcgcgagca cgaggatgtg gatcgtccac gccgcgatgc tgcccaacag cgcgtaccag
     3961 acctcgaacg cgttgcgcac cgcggaccag atgctgtgcg accggcgcgc agcctcggcg
     4021 gtggtgtgca tcgtcatcgg atatgggccg ccaggaacag cgaggggaac acgagtagcc
     4081 acacgccgtc gacgaagtgc cagtagagcg cgaacacctc ggcgctcgcg tgtgcaccgc
     4141 ggtcgtagcg accgagcgcc gccttgatct ggacgacgat gctcatcccg aggccgacga
     4201 cgacgtgcgc cgcgtgcaac ccgacgatcg tgtagaaggc cgacccgtac gcgttgtcgc
     4261 gccagccgaa gtcgaggtgg tggaagtcgt agaacgtgta gcccaagaac gcgaggccca
     4321 tgaggaagct gatgagcatc ccgatccgga accgatcgac gcgcccgttg cgcaggttgg
     4381 cctcggccca gaagagcgga atgctcgaac cccagagcac gaagctgaag gggaacgcga
     4441 gtgcgagctc gggcggatcg atgccgggcg gcggccacgt gtcggcggcc gcgcgcaaga
     4501 agaagtacga ggcgatcaag atcgcgaaga ccatggcctc ggtcatgatg agcaccgcca
     4561 tgccccacca cgccgtcgaa tagccgcggc gcgtcgaagg accgacggtc gtaccggtcg
     4621 cggtcatatc aggtcctctt ccgtgcgcca cgtccaccag aggacgccga cgagcgcgag
     4681 aatcgcaccg acgatcgcga cgagcgtcgc gttcgcgagc aacccgaaga agaagagcat
     4741 cacgccgaac gcggccgcga ccggaaggta ggtctcccgc gggattcgca ggttgccctc
     4801 gaccatcgtg tcgatgccgc tcgtgaccgg tgtctcgcgc cggaccgcgc cttccgctcc
     4861 gagcgactcg gtctcgggct cggtccccga ttgcgcgtag cgcagcgggg tgtcctgcca
     4921 caacggatgc cggctctcga cgatcgggat cgcggcgaag ttgtaatgag gtggcggcga
     4981 cgggacggac cactccaacg agtcgccgtc ccacggattc gcgggcgcgg gtgcaccgcg
     5041 caggcggctg tgcacgaagt tccacaacgt gatcgcggtg ccgagcccga agatcgcgga
     5101 gccgatgctg accatcaggt tcaagccgtc cca
//
LOCUS       DASUGA010000018         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1661619, whole genome shotgun sequence.
ACCESSION   DASUGA010000018 DASUGA010000000
VERSION     DASUGA010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661619"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VFX21_00350"
     CDS             <1..838
                     /locus_tag="VFX21_00350"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX5094422.1"
                     /translation="GAVLRDDDGFAFMTEEHPLADLAPRDVVARAITRRCLERGLDHM
                     WLDATGIRNFADRFPTIWRSVRAVGLDPAHDFLPVAPAAHYLSGGVVADLDGASNLGG
                     LWACGEAACSGVHGANRLASNSLLDGLVFGARIVEAIAAGKDAPEPTGAMRGVARWTQ
                     PETGDIDAITIGASVSIREELQRIMTRDAGVLRDDHRLTRAGGALASMQPESAEDANL
                     LTVGRALVAAALTREESRGTHTRLDFPERSPRFDGRLVFSHGFTPTLVPLPGFEAAEP
                     AP"
     gene            835..1695
                     /gene="nadC"
                     /locus_tag="VFX21_00355"
     CDS             835..1695
                     /gene="nadC"
                     /locus_tag="VFX21_00355"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013654070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX5094423.1"
                     /translation="MNGHREFDPPRQFVVDAVARAIADDVGPLGDLSAALLPHDDRAV
                     ADFIPRAPGVLAGVACATETFAQLDPTVRVTWAETDGASIAKGQSIGRLEGPLRSILT
                     GERTALNFLCHLSGIATLTRQFVDRAGDVRVLETRKTLPGLRALEKAAVRAGGGANHR
                     GTLSDMVLIKDNHLVGTTIAAAVGRARELWPGRGIEVECDRADQVEEAVAAGVDIVML
                     DNMTPDDAAKCVEIVRTSTRPETLVEISGGVSLDTVASYSTTGADLLSTSMITQSAPA
                     LDIGLDLGAE"
     gene            1737..>2010
                     /locus_tag="VFX21_00360"
     CDS             1737..>2010
                     /locus_tag="VFX21_00360"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF015280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX5094424.1"
                     /translation="MLLCIDVGNTQMVIGLFDGRELADHWRIATVAERTSDELALMMA
                     QFLGFHGFAFDEHITGVAVCSGVPRVTGELREMTERYFGYPALVLEP"
BASE COUNT          280 a          735 c          687 g          308 t
ORIGIN      
        1 aggtgcggtc ctgcgcgacg acgacgggtt cgcgttcatg accgaggagc atccgctcgc
       61 cgacctcgcg ccgcgcgacg tggtggcgcg cgcgatcacg cgccgatgcc tcgaacgcgg
      121 cctcgaccac atgtggctcg acgcgaccgg catccgaaac ttcgccgacc gcttcccgac
      181 gatctggcga tcggtgcgcg cggtcggtct cgaccccgcg cacgacttcc ttccggtcgc
      241 acccgcggcg cactatctct ccggcggcgt cgtcgccgac ctcgacggcg ccagcaacct
      301 cggcggcctc tgggcctgcg gcgaggccgc gtgcagcggt gtccacggcg ccaaccgcct
      361 cgcctcgaac tcgctgctcg acggcttggt gttcggagcg cgcatcgtcg aggcgattgc
      421 cgccggcaag gacgcacccg agccaaccgg tgcgatgcgc ggcgtcgcgc ggtggacgca
      481 gcccgagacc ggcgacatcg acgccatcac gatcggcgcc agtgtctcga ttcgcgagga
      541 gctgcagcgg atcatgacgc gcgatgcggg cgtccttcgt gacgaccatc gtttgacgcg
      601 cgcgggcggc gcgctcgcaa gcatgcagcc cgaatcggcg gaggacgcga acctgctcac
      661 cgtcgggcgc gcgctcgtcg ccgccgcgct cacccgtgag gagtcgcgcg gcacgcacac
      721 gcggctggac ttccccgagc ggtcgccgcg cttcgacggc cgtctcgtgt tctcgcacgg
      781 gttcacgccc acgttggtgc ccctgccggg attcgaggcc gcggagccgg cgccgtgaac
      841 ggccatcgcg agttcgatcc gcctcgtcag ttcgtcgtcg acgcggtggc gcgcgcgatc
      901 gccgacgacg tcgggcccct cggtgatctc agcgctgcgc tgctcccgca cgacgaccgc
      961 gccgtcgccg acttcatccc ccgagcgccc ggtgtgctcg caggcgtcgc gtgcgcaacc
     1021 gagacgttcg cgcagctcga tcccacggtg cgcgtcacgt gggccgagac cgacggcgcg
     1081 tcgatcgcga agggtcagtc gatcgggcgg ctcgagggtc ccttgcgatc gatcctcacc
     1141 ggcgagcgca cggcgctgaa cttcctgtgc cacctctcgg ggatcgccac gctcacgcgg
     1201 cagttcgtcg accgcgccgg ggacgtgcgc gtcctcgaga cccggaagac cctccccggg
     1261 ttgcgcgcgc tcgagaaggc tgccgtgcgc gccgggggcg gtgcgaacca ccgcggcacc
     1321 ctctccgaca tggtcctcat caaggacaac cacctcgtcg gcaccacgat cgcggccgca
     1381 gtggggcgtg cgcgtgagct gtggcccggg cgcggcatcg aggtcgagtg cgaccgagcc
     1441 gaccaggtcg aggaagcggt tgccgccggc gtcgacatcg tgatgctcga caacatgacg
     1501 ccggacgatg ctgcgaagtg cgtcgagatc gtgcgcacca gcacccgccc cgagacgctg
     1561 gtggagatct ccggcggagt gtcgctcgac accgtcgcgt cgtactcgac gaccggtgcc
     1621 gatctcctgt ccacgagcat gatcacgcag tccgcacccg cactcgacat cggtctcgat
     1681 ctcggcgccg aatagggtcg ggtcgtcaca tccgtcctcg gcgaaccgga agcgccatgc
     1741 tgctctgcat cgacgtcggc aacactcaga tggtcatcgg cctgttcgac gggcgggagc
     1801 tcgcggacca ttggcgcatc gcgaccgtcg ccgagcgcac ctccgacgag ctcgcgctga
     1861 tgatggcgca gttcctcggc ttccatgggt tcgcgttcga cgagcacatc accggtgtcg
     1921 cggtgtgctc cggtgttccc cgcgtcaccg gcgagctgcg ggagatgacc gagcgttact
     1981 tcggctaccc cgcgctcgtg ctcgagccgg
//
LOCUS       DASUGA010000019         2908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1799283, whole genome shotgun sequence.
ACCESSION   DASUGA010000019 DASUGA010000000
VERSION     DASUGA010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1799283"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFX21_00365"
     CDS             <1..294
                     /locus_tag="VFX21_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019853608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5094425.1"
                     /translation="IALRAAAKAAELPPLRIGVAAGLVIPRDGDYYGPVVNLASRLTD
                     IAEPDEVLVPADLVGELETEGAHAMAGLEVAALGSRQLRGIGAVEVFSVTASH"
     gene            306..1193
                     /gene="mca"
                     /locus_tag="VFX21_00370"
     CDS             306..1193
                     /gene="mca"
                     /locus_tag="VFX21_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003981952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010126 - mycothiol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol conjugate amidase Mca"
                     /protein_id="HEX5094426.1"
                     /translation="MTDRLCLLQVHAHPDDEASKGAGTTAKYAAEGVHNVLVCCTGGE
                     AGDILNPAVEDPGSPEAMHALRMSELEESVRVLGYGSLHLLGYHDSGMPDTETNARSD
                     NFANAPLEEAVGRLVKIIRAERPQVIITYREDRNFYPHPDHIRVHEITIPAFDAAGDP
                     EKYPDAGEPWQPLKLYYVSWSIARVKALHNTYLQRGEESPYESWFERGFDENRDDEFT
                     TLIDVGDYLHKRREALLAHRTQVDPNGFWMRLPDDVIREVFPWEEFTLARSLVDVERR
                     DGEFEDDLFAGIRTVERSQ"
     gene            1190..1489
                     /locus_tag="VFX21_00375"
     CDS             1190..1489
                     /locus_tag="VFX21_00375"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX5094427.1"
                     /translation="MIVAFVVAAGKKEQGRSWANVDDRGAVAEQGEGAPPADADITFT
                     ATPADAQALHDGTLELSVGFMRGQVKMAGDFGALLQFLPRTHGAGATVPVGELLP"
     gene            complement(1486..2679)
                     /locus_tag="VFX21_00380"
     CDS             complement(1486..2679)
                     /locus_tag="VFX21_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX5094428.1"
                     /translation="MTALPLAGLKIVDLSIALTGPYAAALLGDQGASVVKVERPGFGD
                     IGRYVGVSVNGLSALAQMCNRGKRSIALDIHQPEGRAIFLDLARDADVVVQNFRPGVV
                     ERLGIGFEDVRAINSKVIYASLSGFGATGPYAHKGAYDTVIQAYGGFAANQADGETGE
                     PRFLNQTAADKVTALYAAQAITAALFARARTGEGTHLQLTMLDAVVSFLWADAAGNEV
                     MQDADRSQPSSFSANFRPFRFVDGWGICTPTSDADFAGMCQSFGVEGYDAPEVATMMA
                     RAQHREQVRAMLDQCYAAAATMTTAEAIRELESRKVPCGVVIAPSDLAGDPHAQAIGL
                     LEDSKHPTAGALRQPRHPALFGDVRPPLGGGAPMLGEHTDEILGELGLGADRIAELRA
                     AGIAG"
     gene            complement(2676..>2908)
                     /locus_tag="VFX21_00385"
     CDS             complement(2676..>2908)
                     /locus_tag="VFX21_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094429.1"
                     /translation="VARTAAWLAQHPCAPGGQEEMVLTDPFDYAAEDQLIADWHAARA
                     SVSIPSFSSPPGYGLAYSGPGGRPRSNPEFNA"
BASE COUNT          478 a          942 c         1006 g          482 t
ORIGIN      
        1 atcgctttac gcgccgccgc gaaagccgct gaactcccgc cgttgcgaat tggcgtcgcc
       61 gccgggctgg tgatcccgcg cgatggcgac tattacggcc ccgtcgtgaa cttggcgagc
      121 cgcctgaccg acatcgccga gcccgacgag gtgctggttc cggccgatct ggtcggcgag
      181 ctcgagaccg aaggtgcaca cgcgatggcc ggattggaag tcgcggcgct cggttcgcgg
      241 cagctccggg gtatcggcgc cgtggaggtc ttttcggtca ctgcttccca ctagcgtgca
      301 agcccgtgac cgaccggctc tgcctgctcc aggtgcacgc gcatcccgac gacgaagcgt
      361 cgaagggcgc gggcacgacc gccaagtacg cagcagaggg tgtgcacaac gtcttggtgt
      421 gctgcacggg tggcgaagcg ggtgacatct tgaacccggc cgtcgaggat cccggttcac
      481 ccgaagcgat gcacgcgttg cgcatgtcgg aactcgagga aagcgtgcgc gtgctcgggt
      541 acggctcgtt gcacttgctc ggataccacg acagcgggat gcccgacacc gagaccaacg
      601 cgcgttccga caacttcgcg aacgcgccgc tcgaggaggc tgtcggccgg ctcgtcaaga
      661 tcattcgcgc cgaacggccg caggtgatca tcacgtaccg ggaggaccgc aacttttacc
      721 cgcacccgga tcacatccgc gtccacgaga tcaccatccc tgcgttcgat gcggccggcg
      781 atccggagaa gtaccccgac gcaggcgaac cgtggcagcc cctgaagctc tattacgtct
      841 cgtggtcgat cgcccgggtc aaagcgttgc acaacacgta cttgcaacgg ggcgaagagt
      901 cgccgtacga gagctggttc gagcgcggct tcgacgaaaa ccgtgacgac gagttcacga
      961 cgctcatcga tgtcggcgac tacctccaca agcggcggga ggcgctgctt gcgcaccgca
     1021 cgcaggtcga cccgaatggg ttctggatgc ggctccccga cgatgtgatc cgtgaagtct
     1081 tcccctggga ggagttcacg ctggcccgca gcttggtcga cgtcgagcgc cgtgacggcg
     1141 agttcgaaga cgatctcttc gccggaattc gcaccgtgga gcgctcgcag tgattgtcgc
     1201 cttcgttgtc gcggcgggga agaaggagca aggacgttcc tgggcgaacg tcgacgatcg
     1261 tggtgccgtt gccgaacagg gcgagggtgc gccgcccgcc gatgccgaca tcacgttcac
     1321 ggcgacgccc gccgacgcgc aggcgctgca cgacggaacg ttggaacttt ccgtcggttt
     1381 catgcgcggc caagtcaaga tggcgggcga cttcggcgcg ttgttgcagt ttttgccgcg
     1441 tacccacggc gccggcgcga cggttccggt cggcgaactg ttgccttaac cggcgatccc
     1501 ggccgcgcgc agttcagcga tccgatccgc gccgaggccg agttcgccga ggatctcatc
     1561 ggtgtgctcg ccgagcatcg gcgcgccgcc gccaagcggg ggacgcacgt cgccgaagag
     1621 cgccgggtga cgcggttggc ggagtgcgcc cgcggtcggg tgcttggagt cttcgagcaa
     1681 gccgattgct tgtgcgtgcg ggtcgccggc gagatcgctc ggcgcgatca cgacaccgca
     1741 cggcactttg cgggattcga gttcgcggat tgcctcggcg gtcgtcatcg tcgccgccgc
     1801 ggcgtagcac tggtcgagca ttgcgcgcac ctgctcgcgg tgctgtgcac gcgccatcat
     1861 cgtggcgacc tccggcgcgt cgtagccctc gacgccgaac gattggcaca tgccggcgaa
     1921 gtccgcgtcc gacgtcggcg tgcagatgcc ccagccgtcg acgaaccgga acggtcggaa
     1981 gtttgccgag aagctcgacg gctgcgaccg gtccgcgtct tgcatgacct cgttgccggc
     2041 tgcgtcggcc cagaggaatg acacgacggc atccagcatc gtcagttgca gatgcgttcc
     2101 ttcgccggtc cgagcccgtg cgaagagggc ggcagtgatc gcctgcgcgg cgtagagcgc
     2161 cgtgactttg tcggcggcgg tctggttgag gaaccgcggt tcgccggttt ccccgtcggc
     2221 ctggttcgcc gcgaacccgc cgtaggcctg aatgacggtg tcgtacgcgc ccttgtgcgc
     2281 gtaggggccg gtggcgccga agcccgacag ggacgcgtag atcaccttcg agttgatcgc
     2341 gcgcacgtct tcgaaaccga tgccgagtcg ctcgacgact ccgggccgga agttctggac
     2401 gacgacgtcg gcatcgcgcg cgaggtcgag gaagatcgcg cgaccttcgg gctgatgaat
     2461 atcgagcgcg atcgaccgct tgcctcgatt gcacatctgc gcaagggcac tgagaccgtt
     2521 gaccgacacg ccgacgtacc ggccgatgtc gccgaagccc gggcgttcga ccttgacgac
     2581 cgacgcgcct tggtcgccga gcagcgcggc cgcgtacgga ccggtcagcg cgatggagag
     2641 atcgacgatc ttgaggccgg ccagcggcaa ggcagtcatg cgttgaactc cgggtttgat
     2701 cgggggcgcc cacccggccc gctgtacgcg agcccataac cgggtggcga tgagaacgag
     2761 gggatcgaga ccgacgcgcg cgccgcgtgc cagtcggcga tcaactgatc ctcggccgcg
     2821 taatcgaagg gatccgtgag caccatctct tcctggccgc cgggggcaca aggatgttgc
     2881 gcgagccagg ccgcagtccg ggcgaccg
//
LOCUS       DASUGA010000020         2236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_826215, whole genome shotgun sequence.
ACCESSION   DASUGA010000020 DASUGA010000000
VERSION     DASUGA010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826215"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VFX21_00390"
     CDS             <1..949
                     /locus_tag="VFX21_00390"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX5094430.1"
                     /translation="GLMLVFRALEVGGQTLMPSFTFSASAHAVAWNGSEPRFTECDPD
                     TFQMDVDDGVERAKDVDAVLATHVFGAPCNIDALVEAAQRFDVPLVFDAAHAFGARLG
                     GRPVAQFGTASIFSMTPTKPLVAGEGGLVATSDSDLAHRVRIGRDYGNPGNYDTQFVG
                     LNGRMSEMHAATALASLDTFTEQQRTRDDIALRYRNALRSVPGIRVQHIPEADTSTFK
                     DLTIAISPGEFGVDRDTLRVALHAEGVDTRCYFDPPVHRQTAYSAVASGPLPVTERVA
                     GEVLSLPIYPDLSLHAVDRIVDLIATVHDFADEVRDRPS"
     gene            1351..>2236
                     /locus_tag="VFX21_00395"
     CDS             1351..>2236
                     /locus_tag="VFX21_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX5094431.1"
                     /translation="MRGPNSNGRYEARLEGQAGVAVYSSAGVTLDRVNVRATYGDGVY
                     LAGDSSRITVRDGIFERLGRQGIAPVFASDVVVEANRFDRVARSVFDLEPAVPRWSVR
                     DVHLRANTVGTYGNFVLAAGGAGENVSGIWLEANEVTGGNGIAVYAGMPRWLRHDLHV
                     VDNRSRVAGRVVGATGRAGVMQIARIDGVEIRGNEQPIADDGVAISLTDVCHADVRDN
                     DFPGASEAVRRTGDTENCSPELRGGPPSSRPKGTTTPDRAGRRAETPRSPPVEDAVGR
                     DWLIALILGGLAAFAVFLA"
BASE COUNT          352 a          742 c          765 g          377 t
ORIGIN      
        1 cggcctcatg ctcgtgtttc gcgcgctcga ggtcggcggc cagacgctca tgccgtcgtt
       61 cacgttctcg gcgtccgcgc acgcagtcgc gtggaacggc tccgagccgc gctttaccga
      121 atgcgacccg gacacgttcc agatggatgt cgacgacggg gtcgaacggg cgaaggatgt
      181 cgatgccgtg ctggcaaccc atgtcttcgg agcgccgtgc aacatcgacg cgctcgtcga
      241 ggcggcgcag cggttcgacg ttccgctcgt cttcgacgcc gcgcacgcct tcggggcgcg
      301 tctgggcgga cggccggttg cccagttcgg gaccgcctcg atcttcagca tgactccgac
      361 caagccgcta gtggccggcg agggtggcct cgtcgcgacc tctgactccg atcttgcgca
      421 ccgcgtccgg atcggccggg actacggcaa cccggggaac tacgacacgc agttcgtggg
      481 cctcaacggc cgcatgtcgg agatgcacgc tgcaaccgcg ctcgcgtccc tggacacctt
      541 caccgagcag cagcgcacgc gcgacgacat cgcacttcga taccgcaacg cactgaggag
      601 cgtccctggc attcgggtac agcacattcc cgaggccgac acgtcgacct tcaaggactt
      661 gacgatcgca atttcgcccg gcgagttcgg cgtcgatcgc gacacccttc gggtcgcgct
      721 tcacgccgaa ggcgtcgata cgcggtgtta cttcgatccg cctgttcacc gccagacggc
      781 gtattccgcg gttgcatcgg gcccgctccc cgtgaccgaa cgcgtcgcgg gcgaagtgct
      841 gagcctgccg atctaccccg acctttccct gcacgcggtc gatcgcatcg tcgatctgat
      901 cgccacagtg cacgacttcg cggacgaagt gcgggaccgt ccttcttgag gttcgccctg
      961 tcggtccggc tggcgtgcgc gctcgccgtc gtagcaagtc tgattgcatc ggccgcgccg
     1021 gtggcgaacg ccgcaggtgt tgcggttccg gccgcgatcg acgcatcggg aacggccgat
     1081 gtgaccaacc agctcaacga cttcttagca aacatcgcgg cgggaacgac ggtgaccctt
     1141 cccgcgcggg cgcgctatcg cgtcgaaggc gtgttgcagt tggccgaatt gcacgacgtc
     1201 acgatcgcgg gcaatgaggc gactatcttc gcgacgaccg acggatcgag tgtcccgccg
     1261 ccgcggggtc gcttccgatc acgctggccg cgtctgcgcg agcacctctc gatcaaggat
     1321 gcgagcaatc tgacgatccg cggcctcaac gtgcgcgggc ccaacagcaa cggccgctac
     1381 gaagcacggc tcgaaggcca agccggtgtc gccgtgtatt cgtcggccgg tgtcacgctg
     1441 gaccgcgtca acgtgcgtgc gacctacggc gatggtgtct acctcgctgg ggactcgagc
     1501 cggatcacgg tgcgagacgg aatcttcgaa cggctcggtc gccaaggcat cgcgccggtc
     1561 ttcgcgtcgg acgtcgtcgt ggaggcgaat cggttcgacc gcgtcgctcg gtccgtcttc
     1621 gacctcgaac ccgccgtccc ccgctggtcg gtgcgcgacg tgcacctgcg tgcaaacacg
     1681 gtcggaacct acggaaactt cgttctggcg gccggaggcg cgggtgagaa cgtgagtggc
     1741 atctggttgg aggccaacga agtcacgggc ggcaacggga ttgcagtgta cgcagggatg
     1801 ccgcggtggc tgcgccacga cctgcatgtg gtcgacaacc gcagtcgcgt cgccggccgg
     1861 gtcgtgggag ccaccggtcg ggcgggcgtg atgcagattg cgcgcatcga cggcgtggag
     1921 attcggggaa acgagcagcc cattgccgac gacggggttg ccatctcact cacggacgtc
     1981 tgtcacgcgg acgtgcggga caacgacttt ccgggggcgt ccgaggccgt gcgccgaacc
     2041 ggagacacag aaaactgctc gcccgagctg cgaggcggcc cgccgtcgtc gcggccgaag
     2101 gggacgacga cgccggaccg ggcggggcga agggctgaga ctccgcggtc cccaccggtc
     2161 gaggacgcgg tcgggcggga ttggctgatc gccctgatct tggggggcct cgcggcattt
     2221 gcggtgtttc ttgccg
//
LOCUS       DASUGA010000021         2964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1103603, whole genome shotgun sequence.
ACCESSION   DASUGA010000021 DASUGA010000000
VERSION     DASUGA010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103603"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..367
                     /locus_tag="VFX21_00400"
     CDS             140..367
                     /locus_tag="VFX21_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094432.1"
                     /translation="MTKAADVRAELGHSVVDADGHHIEYLPDARAVLPEAFELLERGR
                     VDAAQFRAFTCDNARRCWGDTVFTGTAAEFG"
     gene            377..1243
                     /locus_tag="VFX21_00405"
     CDS             377..1243
                     /locus_tag="VFX21_00405"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX5094433.1"
                     /translation="MLLSKATLDAYRRDGFVVVDGLLSDAELDHYEQHVTAAVAHRTR
                     ADERALGDRSPYEQSFRQCINLWEDFPEVEPLTFHPRLAQAASELVGKDVMRLWHDQA
                     LYKEAGGRETDPHQDQPYWPMTETDTVTAWIPFRGSTLASGAMGYLPGSHLVGVRKFV
                     NIFQADDAGVLMELPEIKAIVPMWVEVPRGSVAFHSGLTVHMAKPNTTGTDRAVHTVI
                     YFADGSTRRNAAFHPSVDRDEIEVGAVIRGSCTPVVWPRADGDPVPRPAPIPEFVRQV
                     AGHGTLPEERVR"
     gene            1282..1917
                     /locus_tag="VFX21_00410"
     CDS             1282..1917
                     /locus_tag="VFX21_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094434.1"
                     /translation="MADLHELGVAVHGKRTLPEARRRALQLMRGLPAAWWVCGGWAID
                     MFIGHQTRPHADLEVTLLHRDHPLLFTHFAGWELRIPGDGNWARWQGEDLGPEIHQLW
                     ARPDRGTDGQPEDFVRDADYCEILLETATRDEWIYRRDNRLRRRLIDIGREYGSTPVI
                     APDIALLYKAKHRRPHDTADFYEALPHLEPNGLVWLRNAIATAHPECDWFA"
     gene            complement(1972..2430)
                     /locus_tag="VFX21_00415"
     CDS             complement(1972..2430)
                     /locus_tag="VFX21_00415"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX5094435.1"
                     /translation="MSRAESFAALPPERRARWSRFAKWDTPYFPKFIGLDLEEVRRDY
                     ARMRLAYRPEFLQPAGVWHGGVIATMLDTVVVPAVGSAYDEPRALFTIDIALRYLAPL
                     KPDEDAVAEGWITQRGTSIVFCDAEVRSASGTLAATATLTYKVSKRAADY"
     gene            complement(2430..2873)
                     /locus_tag="VFX21_00420"
     CDS             complement(2430..2873)
                     /locus_tag="VFX21_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007027273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5094436.1"
                     /translation="MIRDAEPGDLAEILALVRELAEYERSADDVAFDAEEFGRHVFGP
                     DAVARVLIAEYDGAVAGMALYYRTFSTWVGRPGIWLEDLFVRPAFRRHGLGRALLDAL
                     QARTDGRVEWAVLDWNTPAHDFYRTLGAHPMEEWTVWRWPPVASG"
BASE COUNT          475 a         1094 c          975 g          420 t
ORIGIN      
        1 acctcgtcgt cgacagcgcg caacggctgg cgcgcgcaac gatcgtgacg atcgccggcg
       61 ccgggcactc gccgtacttc gaacgtcccg acgagttcaa cgccgcgctg ctcgaattcc
      121 tcggcccgat tccgtcacag tgacgaaagc ggccgacgtc cgcgccgaac tgggccacag
      181 cgtcgtcgac gccgacggcc accacatcga gtacctgccg gacgcgcgcg ccgtgctccc
      241 cgaggcgttc gagctcctcg aacgcggtcg cgtcgatgcc gcgcagttcc gggcgttcac
      301 ctgtgacaac gcgcgccgct gttggggcga caccgtcttc accggcacgg cggcggagtt
      361 cggataggtt gccggcgtgc tcctgagcaa agcgactctc gacgcgtacc ggcgcgacgg
      421 attcgtcgtc gtcgacggtc tgctctccga cgcggagctc gaccactacg aacagcacgt
      481 caccgcggcc gtcgcgcacc gcacgcgcgc cgacgagcgc gccctcggcg accgctcccc
      541 ctacgagcag tcgttccggc agtgcatcaa cctgtgggag gacttccccg aggtcgagcc
      601 gctcacgttc catccgcgtc tcgcgcaagc cgcgagcgag ctcgtcggca aggacgtcat
      661 gcgcctctgg cacgaccaag cgctctacaa agaagccggc gggcgcgaaa cggatccgca
      721 ccaagaccag ccgtactggc cgatgaccga gaccgacacc gtgaccgcat ggatcccgtt
      781 ccggggttcg acgctcgcga gcggcgcgat gggctacctg cccggatcgc acctcgtcgg
      841 cgtgcgcaag ttcgtgaaca tcttccaggc cgacgacgcg ggcgtgctca tggaactgcc
      901 cgagatcaag gcgatcgtgc ccatgtgggt cgaagtgccg cgcggttcgg tcgcgttcca
      961 ctccgggttg accgtgcaca tggccaagcc caacacgacc ggcaccgacc gcgccgtgca
     1021 cacggtcatc tacttcgccg acggcagcac ccggcgcaac gcggcgttcc atccgtccgt
     1081 cgaccgcgac gagatcgagg tcggtgccgt gatccgcggc tcctgtaccc ctgtcgtgtg
     1141 gccccgcgcc gacggcgacc cggtgccccg ccccgcaccc atcccggagt tcgtccgcca
     1201 ggtggccggc cacggtacgc tgccggagga gcgcgtcagg tagcaatcgg ccgggccgat
     1261 tgcgaacggg tgggcggtca catggcggat ctgcacgagc tgggcgtcgc cgtccacggc
     1321 aagcggaccc tgcccgaagc gcgccggcgg gccctccagc tgatgcgggg cttgccggcc
     1381 gcgtggtggg tctgtggcgg ctgggcgatc gacatgttca tcggccacca gacccggccg
     1441 cacgccgatc tcgaagtgac cctgctccac cgcgatcacc cgctgctgtt cacgcacttc
     1501 gcgggctggg agctgcgcat ccccggcgac ggcaactggg cccgttggca gggcgaggat
     1561 ctcggcccgg agatccacca gctctgggcc cgacccgacc gcggcaccga cggccaaccc
     1621 gaggacttcg tgcgcgacgc ggactactgc gagatcctcc tcgaaaccgc gacgcgcgac
     1681 gagtggatct accgccgcga caaccgcttg cgccgccggt tgatcgacat cggccgcgag
     1741 tacggcagca cgcccgtcat cgcgcccgac atcgcgttgc tctacaaggc caagcatcgg
     1801 cggccgcacg acaccgccga cttctacgaa gcgctccccc acctcgaacc gaacggcctg
     1861 gtgtggctgc gcaacgcgat cgcgaccgca caccccgagt gcgactggtt cgcgtgaaca
     1921 ccctcgggcg tcttacaagg gcggattccg cccttgtaag acgcccgaga atcagtagtc
     1981 ggcggcgcgc ttggagacct tgtacgtcag ggtcgcggtt gccgcgagcg tgcccgatgc
     2041 cgagcgcacc tcggcgtcgc agaacacgat cgacgtgccg cgctgggtga tccagccttc
     2101 ggcgacggcg tcttcgtcgg gcttcaacgg cgcgaggtag cgcaaggcga tgtcgatggt
     2161 gaacaacgcg cggggctcgt cgtacgcgct cccgaccgcg ggcacgacca ccgtgtcgag
     2221 catcgtcgcg atgaccccgc cgtgccacac cccggccggc tggaggaact cgggccggta
     2281 cgcgagccgc atgcgcgcgt agtcgcggcg cacttcttcg aggtcgagcc cgatgaactt
     2341 cgggaagtac ggcgtgtccc acttggcaaa acgcgaccac cgggcacgcc tttcgggcgg
     2401 cagcgcagcg aacgactcgg cgcgtgacat cacccggagg ctacgggagg ccacctccag
     2461 accgtccact cctccatcgg atgcgcgccc agcgtgcggt agaagtcgtg cgcgggcgtg
     2521 ttccagtcga gcaccgccca ctcgacgcgg ccgtcggtgc gcgcctgcag cgcgtcgagc
     2581 aacgcgcgcc ccaagccgtg ccggcggaac gccgggcgta cgaacaggtc ttcgagccag
     2641 atgccgggcc ggccgaccca cgtcgagaac gtgcggtaat acagcgccat ccccgcgacc
     2701 gcgccgtcgt actccgcgat cagcacgcgc gcaacggcgt cgggcccgaa cacatggcgg
     2761 ccgaactcct ccgcgtcgaa cgcgacgtcg tcggcactgc gttcgtactc ggccagctcg
     2821 cgtacgagcg cgagtatctc cgcgagatca cccggttccg cgtcgcgaat catcagtgcg
     2881 tgacgaacgg cggcttcacg accgtcgccg cgagccgctt gccgcgcacc tcgagctcga
     2941 cggccgcgcc cgcctcgatc gcgg
//
LOCUS       DASUGA010000022         3178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_964867, whole genome shotgun sequence.
ACCESSION   DASUGA010000022 DASUGA010000000
VERSION     DASUGA010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964867"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..575)
                     /locus_tag="VFX21_00425"
     CDS             complement(30..575)
                     /locus_tag="VFX21_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5094437.1"
                     /translation="MATTFTRMDQSTAEQWAVIGKETYEHQGRVAESVLEMLRSLAGI
                     TDGFAVDQLTHSLQTATLAERAGADPDVVFASLLHDIGKAVSVPNHPEIAASILKPYV
                     RDEVYQVIRTHQDFQGRHYYAHFGGDPNARDQYQGHEAFALAERFADEWDQQAFDPEY
                     DTLPLEHFEARVRALFDSAKF"
     gene            complement(591..1298)
                     /locus_tag="VFX21_00430"
     CDS             complement(591..1298)
                     /locus_tag="VFX21_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094438.1"
                     /translation="MLEALPESFANTRQALHALAEHVIAPARYRADGRIGLVPTANGF
                     GTPAFGDRERVRVEGIELVHERPGTTTRIGITTLHDAAAFLGIPLGAPAEVYTPETTI
                     QPDVALAVDAAGAKALAEWYALGAAALDALLATYSAHEPSGMTLWPEHFDLAAVMGDA
                     DAGTRANYGASPGDAVIETPYLYVGPWEAARRVGPFGAHPFGAALTYEELRAAPDAKA
                     RATEFMLECAALLVGQP"
     gene            complement(1308..2210)
                     /locus_tag="VFX21_00435"
     CDS             complement(1308..2210)
                     /locus_tag="VFX21_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5094439.1"
                     /translation="MAAAHYDEFSLFHENAEEFGIAWDGPPAVRRSAVDVGNGQMISA
                     LVWGTGSPELVLIHGGAQNAHTWDTVALALDVPLIAFDLPGHGHSSHRDDHAYWPADN
                     AAAIERAMRELAPDARVVVGMSLGGLTALALTDRAPDLVQKLVLVDVTPGVNREKSTA
                     IAQFIDGPEYFESFDEILHRTILFNPTRSESSLRRGVLHNAIEADDGRWRWRYDLPRR
                     GSGEGDEGSIMPGLDDLWDAVGRVKVPFLLLRGSTSPVVDDEDVAELNRRNPDARVIV
                     VEGAGHSIQGDKPIELAEILRTLL"
     gene            2283..>3178
                     /locus_tag="VFX21_00440"
     CDS             2283..>3178
                     /locus_tag="VFX21_00440"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX5094440.1"
                     /translation="MRAPRVSPHALRRLALVNIVLLVAIIVSGAIVRLSRSGLGCADW
                     PNCSSTKFVDVSSGHSAIEQLNRIFSGLLGIPLFLGVLFAYWRTPRRRDLVRLSWWLF
                     GLFWMNAVVGGISVMIKLAWFSVTTHFLLAITLVALALVVRQRASEPEGPYRALVPAR
                     TVALARVVYGLTVVVLVLGTLVTAAGPHGGDDEAERLGWNLGDIARVHGIAVDILVVL
                     TIAFGVVLVRVHAPRRALTWVSITIATMAAQGILGYTQYFNKIPAVLVGFHVFGAVCV
                     FVCVQQLLLELRAAEGPSAVAP"
BASE COUNT          470 a         1051 c         1100 g          557 t
ORIGIN      
        1 caggagcgac accgatcaga acgtcccgtt cagaacttgg cgctgtcgaa gagcgcccgg
       61 acgcgcgcct cgaagtgctc gagagggagc gtgtcgtact cggggtcgaa cgcctgctgg
      121 tcccactcgt cggcgaagcg ctcggcgagc gcgaacgcct cgtggccctg gtactggtcg
      181 cgtgcgttcg gatcgccgcc gaagtgcgcg tagtagtggc ggccctggaa gtcctggtgg
      241 gtgcggatca cctggtacac ctcgtcgcgc acgtagggct tgaggatcga cgccgcgatc
      301 tccgggtggt tcggcacgct cacggccttg ccgatgtcgt gcaacagtga cgcgaacacg
      361 acatcggggt cggcaccggc gcgttcggcg agcgtcgcgg tctggaggga gtgcgtcagc
      421 tggtcgaccg cgaagccgtc ggtgattccg gccaacgagc gcagcatctc aagcacgctt
      481 tcggcgaccc ggccctgatg ctcgtaggtc tccttgccga tcacggccca ctgttcggcc
      541 gtcgactggt ccatgcgcgt gaacgtggtt gccatgcgcg aaaggttacg tcaaggctgg
      601 ccgacgagca gcgctgcaca ctcgagcatg aactccgtgg cccgggcctt ggcgtcgggt
      661 gcggcccgca gctcctcgta cgtcagggcc gcgccgaagg ggtgcgcgcc gaacggcccg
      721 acgcggcgcg cggcttccca cggcccgacg tagagatacg gcgtctcgat cactgcgtcg
      781 ccgggcgacg cgccgtagtt ggctctcgtc cccgcgtcgg cgtcgcccat gacggccgcg
      841 agatcgaagt gctcgggcca gagcgtcatg ccactgggtt cgtgggcgga ataggtcgcg
      901 aggagcgcgt caagggcggc ggcgcccaac gcgtaccact ccgccaaggc tttggcaccc
      961 gcggcgtcga ccgcgagcgc gacatcgggc tggatggtgg tctcgggtgt gtacacctcg
     1021 gccggcgcgc ccaacggaat ccccaagaat gcggcggcgt cgtgcagcgt cgtgatcccg
     1081 atccgcgttg tcgtgccggg ccgttcgtgc acgagttcga tgccttcgac gcgcacccgt
     1141 tcgcggtctc cgaatgccgg cgtgccgaag ccgttcgcgg tcggcacgag cccgatgcga
     1201 ccgtcggccc gatagcgcgc cggcgcgatg acgtgctcgg ctaacgcgtg cagcgcctga
     1261 cgcgtgttgg cgaaggattc gggaagggct tcgagcaccg gggcatgcta aaggagcgtg
     1321 cgcagaatct cggccaactc gatgggcttg tcaccctgaa tcgagtgccc ggcaccttcc
     1381 accacgatga cgcgtgcgtc gggattccgg cggttgagtt cggcaacgtc ctcgtcgtcg
     1441 acgacggggg aggtgctgcc ccgcaagagc aggaagggaa ccttcacccg gccgactgcg
     1501 tcccagaggt cgtcgagccc gggcatgatg ctgccttcgt cgccttcgcc cgaaccacga
     1561 cgcggcaagt cgtaacgcca gcgccaacgg ccgtcgtccg cctcgatcgc gttgtgcaag
     1621 acgccgcgtc gcaacgacga ctcgctgcga gtcgggttga acaagatcgt gcgatggaga
     1681 atctcgtcga agctctcgaa gtattccggt ccgtcgatga actgcgcgat ggccgttgac
     1741 ttctcgcggt tgactcccgg cgtgacgtcg acgaggacga gcttctgcac gaggtcgggg
     1801 gcgcgatccg tgagcgccag cgcggtgagg ccgccaagcg acatgccgac gacaacccgg
     1861 gcgtcgggcg ccaactcgcg catggcgcgc tcgatcgcag ccgcgttgtc ggccggccag
     1921 tatgcgtggt cgtcgcggtg cgaggaatga ccgtgaccgg gcagatcgaa cgcgatgagc
     1981 ggaacatcga gtgcgagtgc gacggtgtcc caggtgtgcg cgttctgcgc gccgccgtga
     2041 atcagtacca gctcgggcga cccggtgccc cagacgagtg cgctgatcat ctgcccattg
     2101 ccgacgtcga ccgcgctccg ccgtaccgcc ggcgggccgt cccaggcgat gccgaactcc
     2161 tcggcatttt cgtggaacag cgagaactcg tcgtagtgcg cggcggccat aggggtgcag
     2221 tcgagtccac cagggccggt cattgcaaga ctcgggaaga ccccttccta cactcggagg
     2281 cgatgcgtgc ccctcgggtg tcgccgcacg cgctgcgtcg cctcgctctc gtcaacatcg
     2341 tgctgctcgt cgcgatcatc gtgagcggcg cgatcgtgcg gttgtcgcgt tcgggcctgg
     2401 gctgtgccga ctggcccaac tgcagttcga cgaagttcgt cgacgtctcg agtgggcact
     2461 ccgccatcga gcagctgaac cgcattttct ccggcctgct cggcatcccg ctcttcctgg
     2521 gcgtgctgtt cgcgtactgg cggacgccgc gtcgacgcga tctcgttcgg ctctcgtggt
     2581 ggctcttcgg gctcttttgg atgaatgcgg tggtcggcgg catctccgtc atgatcaagc
     2641 tcgcgtggtt cagcgtgacg acccacttcc tgctcgccat cacgctcgtc gcgctcgcgc
     2701 tcgtcgtccg ccaacgggcg agcgaacccg aaggcccgta ccgggcgctc gtccctgcga
     2761 gaacggtcgc gttggcgcgc gtcgtgtacg gcttgacggt ggttgtgctg gtgctcggca
     2821 cgctcgtcac cgcggccgga ccacacggag gcgacgacga agccgaacgc ttggggtgga
     2881 acctcggcga catcgctcgc gtccacggca ttgccgtcga catcctcgtc gtgctcacca
     2941 tcgcattcgg cgtcgtgctg gtgcgcgtgc acgcaccgcg gcgcgcgctg acctgggtgt
     3001 cgatcaccat cgcgacgatg gccgcgcagg gaattctcgg ttacacccag tacttcaaca
     3061 agatcccggc agtgctcgtc gggttccacg tgttcggcgc ggtgtgcgtg ttcgtctgcg
     3121 tccagcagct cctgctcgag ctccgagccg cggaaggacc atccgcggtc gcgccgga
//
LOCUS       DASUGA010000023         2767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_129024, whole genome shotgun sequence.
ACCESSION   DASUGA010000023 DASUGA010000000
VERSION     DASUGA010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129024"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VFX21_00445"
     CDS             complement(<1..623)
                     /locus_tag="VFX21_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5094441.1"
                     /translation="MTPTPRAAYALGIVALVALFVPVELAIALVILVVVLTIVDLAYA
                     RAKVRVRRSMPRALSRGVPAHLSITSDAPRPRQVRVRQANPADVTIAEPVGTAVIDTT
                     VVAHRRGEAVLPPVSVRQTGPLGLGRRTFDGEQPAPLLVYPDVVAANRMVIALRTGRF
                     RDPGLRTRGPLGLGTDFESIRDYLPDDDVRQVNWTASERTGRPMSNV"
     gene            complement(620..1549)
                     /locus_tag="VFX21_00450"
     CDS             complement(620..1549)
                     /locus_tag="VFX21_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016469308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5094442.1"
                     /translation="MNELRERVVREIRKVVVGQDHVVDVLLAATSVGGHLLLEGVPGV
                     AKTLLANAYARALGVEFRRVQFTPDMLPSDLTGTMTLRAGELAFRPGPVFTNLLLADE
                     INRTPPKTQAALLEAMQERQVSVDGVARPLPEPFVVVATQNPIEYEGTYPLPEAQLDR
                     FLAKIDVGYPSAADEAQILRLAHHGVAPATLQDVQPVTTAEELVQVRTLVDATTVAPE
                     VIDYVVALVRRTRELPSVALGASPRSAVHLLAAAKAAARLTGRDFVVPDDVVAVAPAV
                     LRHRITLRPEAELERYRADDVVSGAIGSVPVPR"
     gene            complement(1546..2679)
                     /locus_tag="VFX21_00455"
     CDS             complement(1546..2679)
                     /locus_tag="VFX21_00455"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEX5094443.1"
                     /translation="MSDTEVVAPRRPRRVLVVVGVVVAAIIGLNLVARGLDEAVGGNE
                     PSGRPGSSYATTAEGLAAYATLLTRYDHTVSFQRGKLVDDPPPTDSTVFVMDPEVLTE
                     EDAGVLLTFATNGGRLVIGGPSPFYLSNLRDQPPAWSPVGYSTWDANAPELGGDVTIE
                     TAAYGSWTEPGSSRPVVRDHRAALITTERVGVGEIWFVADPSFVTNEFLAREDNATVG
                     IELAGDRPVIFAEGVHGFGETRGFSAIPANWKWALFLLGVAALALVWSRARRFGPPNV
                     DARDLPPARSEYVEALSTTLERTHQPAAAMASTQAYARDRIAARTALGAEPNPEQIVA
                     AARVLGCTDEEIAALSTPVVDDAHVLALGRAVARVTSGDRRME"
BASE COUNT          434 a          964 c          962 g          407 t
ORIGIN      
        1 tacacgttgc tcatcggccg gccagtgcgc tccgaagcag tccagttgac ctggcgcacg
       61 tcgtcgtcgg gcaggtagtc gcggatcgat tcgaagtcgg tgccgagccc caacggcccg
      121 cgcgtccgca gaccgggatc gcggaagcga ccggttcgca acgcgatcac catgcgattg
      181 gccgcgacga cgtccggata cacgagcagc ggcgccggtt gctcgccgtc gaacgtgcgg
      241 cgtccgagac cgagcggacc ggtctggcgc acgctgacgg gcgggagcac cgcctcgccc
      301 cgccggtgcg caacaacggt tgtgtcgatg accgcggtgc cgaccggctc ggcgatcgtg
      361 acgtcggccg ggttcgcttg gcgaacacgc acctgtcgcg ggcggggcgc gtcgctcgtg
      421 atgctcaagt gcgcaggcac accgcgcgac agcgcgcgcg gcatgcttcg ccggacgcgc
      481 actttggcgc gcgcgtacgc aaggtcgacg atcgtcaaca cgaccaccag gattaccaac
      541 gcaatcgcga gctcgaccgg cacgaacagc gcaacgagcg caacgatccc gagcgcgtac
      601 gccgcgcgcg gcgtcggcgt caccggggaa ccggtaccga gccaatggcg cccgagacaa
      661 cgtcgtcggc ccggtagcgc tcgagttccg cttcgggccg caacgtgatg cggtgccgca
      721 ataccgcggg tgcgaccgcg acgacatcgt cggggaccac gaagtctcgg ccggtgagcc
      781 gggccgcggc cttggcggcg gccaggagat gcacggccga tcgcggactg gcgccgagcg
      841 cgacgctcgg cagttcgcgc gtgcgccgca ccagcgcgac gacgtagtcg atgacctcgg
      901 gcgcgaccgt tgtcgcgtcg acgagcgtgc gcacctgcac gagttcctcg gccgtggtca
      961 cgggttgcac gtcctgcaac gtcgcgggcg ccacaccgtg gtgcgcgagc cgaaggatct
     1021 gcgcttcgtc ggccgcactc ggatagccga cgtcgatctt ggcgaggaac cggtcgagct
     1081 gtgcctcggg caaggggtat gtgccttcgt actcgatcgg attctgcgtc gcgacgacaa
     1141 cgaacggttc gggcagcggt cgcgcaacgc catcgacgct cacctgccgc tcctgcatcg
     1201 cttcgagcaa cgcggcttgt gttttgggtg gtgtgcggtt gatctcgtcg gcgagcaaaa
     1261 gattggtgaa caccggcccg ggccgaaacg ccagctcgcc cgctcgcagc gtcatcgttc
     1321 cggtgaggtc ggagggcaac atgtcgggcg tgaactgcac ccggcggaac tcgacgccga
     1381 gcgcgcgggc gtaggcgttg gcgagcaacg ttttcgcaac gcccggcacg ccctcgagga
     1441 gcaagtgccc gccgacgctg gtggccgcga gcagcacatc gaccacgtgg tcttggccga
     1501 ccaccacctt gcggatctct cggaccacgc gttcccgcag ctcgttcact ccatcctcct
     1561 gtcaccactg gtcacgcgcg caaccgcgcg gcccaacgcc aagacgtgtg catcgtcgac
     1621 aaccggtgtc gacaatgccg cgatctcctc gtcggtgcag ccgagcacgc gcgccgccgc
     1681 gacgatctgc tccggattcg gttcggcgcc gagcgcagtg cgtgccgcga tgcggtcccg
     1741 ggcgtaggcc tgggttgacg ccatagccgc cgcgggctgg tgcgtgcgct ccaacgtcgt
     1801 cgacagcgcc tcgacgtatt cggagcgcgc cggcggtaag tcgcgcgcgt caacattggg
     1861 cggcccgaac cggcgcgcgc gtgaccacac caatgccaac gcggccacgc cgagcaggaa
     1921 cagcgcccac ttccaattgg ccgggatggc cgagaaccct cgcgtttcac cgaagccgtg
     1981 caccccctcg gcgaagatca ccgggcggtc gccggccagt tcgatgccga ccgtcgcgtt
     2041 gtcttcgcgc gccaggaact cgttggtgac gaacgacgga tccgcaacga accagatctc
     2101 gccgacgccg acgcgctcgg tcgtgatgag cgcagcgcgg tggtcgcgga cgaccggccg
     2161 gctgctgccg ggttcggtcc acgacccgta cgccgcagtc tcgatcgtca cgtcgccgcc
     2221 gagttcgggt gcgttcgcat cccatgtcga gtagccaacc gggctccagg ccggcggctg
     2281 gtcgcgcaga ttcgacagat agaacgggga agggccgccg atcacgagcc gcccaccgtt
     2341 cgtcgcaaag gtgaggagca cgcccgcgtc ctcttcggtg agcacctcgg gatccatgac
     2401 gaacacggtc gagtccgtcg ggggcgggtc gtcgaccagt ttcccccgct ggaacgacac
     2461 ggtgtggtcg taacgcgtga gcaacgtcgc gtaggcggcg aggccttcgg cggtcgtcgc
     2521 gtacgaagac ccgggccgcc cgctcggttc gttcccgccg acggcttcgt cgagaccgcg
     2581 cgcgacgagg ttcagcccga tgatcgcggc aaccacgacc ccgacgacga cgaggacgcg
     2641 gcgagggcgg cgcggcgcaa cgacttcggt gtcgctcact tgcggcccgc ctcgtcgagc
     2701 acgcgcggcc agtcttggcg cgcggcggcc acgtcgggtt gctccgcggg cttgccgccg
     2761 tacgtca
//
LOCUS       DASUGA010000024         3478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_964499, whole genome shotgun sequence.
ACCESSION   DASUGA010000024 DASUGA010000000
VERSION     DASUGA010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964499"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VFX21_00460"
     CDS             complement(<1..418)
                     /locus_tag="VFX21_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013936871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX5094444.1"
                     /translation="MTTLGDDARVGLTSTPKDLPPKWFYDDRGSALFDEITRLPEYYP
                     TRAERSILVARAAEIAAATDAGTLVELGSGTSEKTRLLLDALRDHGTLRRFVPFDVSE
                     QTLKDAAAAIAEEYPGIDVEPVVGDFDHDLHRLPRGG"
     gene            complement(415..1623)
                     /gene="egtB"
                     /locus_tag="VFX21_00465"
     CDS             complement(415..1623)
                     /gene="egtB"
                     /locus_tag="VFX21_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEX5094445.1"
                     /translation="MATATAIVDREALRTRFHAIRNFTESLAAPLGPEDQVVQSMPDV
                     SPTKWHRGHTTWFFETFLLQPSLDGYQPYHPAFVYLFNSYYEAVGPRHPRPLRGLLSR
                     PTVEEVARYRAHVDVMMDDLISRCADAIWPDVCALIELGLHHEQQHQELLLMDIKHVL
                     SCNPLEPAYAPNADEPVTAPALRWHDVDGGFATIGHGDTTFCFDNETPQHTVLVEGFS
                     IANRLVTASEWIDFIADGGYTRSELWLSDGWYAVQEHGWNAPLYWDDHDGDWTAFTLQ
                     GRRPLHPGEPVVHVSHYEADAFARWSGARLPTEFEWEHAVSTVPSLAQISGEAWQWTS
                     SAYLPYPRFAPAAGAVGEYNGKFMSGQMVLRGGARITSPDHVRPTYRNFFPPGSRWMY
                     GGVRLARDAS"
     gene            complement(1784..2887)
                     /locus_tag="VFX21_00470"
     CDS             complement(1784..2887)
                     /locus_tag="VFX21_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5094446.1"
                     /translation="MIRLEAVSKRYPNGTLAVHELTLSVPTGETAVLVGPSGCGKTTL
                     LRMINRLIDPTSGRIYLDDEDITDTNPVELRRRMGYVIQQIGLFPHQTIERNIGTVPR
                     MLGWSRARIRERSDELLTLVGLDADEYRDRYPHQLSGGQRQRVGVARALAADPPVLLM
                     DEPFGAIDPVTRARLQDEFVRLQERLQKTIVFVTHDIEEAIKLGDRIAILNIGGHLEQ
                     YDIPSEILGHPATDFVASFVGIDRAIKQLKVTMIDTEYLEHPPTLRPDALLADARQLL
                     GEQGSAVPIVDDHQRLCGRIRSVDADGEGTVAERVARVEAWVDVHDTLEHALAHTLLS
                     DDGWIAVLDDDHFCGVLTPAGVHAALRASLRHS"
     gene            2985..>3478
                     /locus_tag="VFX21_00475"
     CDS             2985..>3478
                     /locus_tag="VFX21_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5094447.1"
                     /translation="MTGVSSGVLAAYDGRAWIDWTWVGDHGDEILARLREHAALTLWA
                     MVLGIVIAFPLALVSVHRRRTRKFVLATTGILYTIPSLAAFALLLPYTGLSRMTAIIP
                     LATYTLLILIRNIVAGLDAVPVEVQDAAVGMGYSSTRKMLRVDLPLALPTIFAGVRIA
                     AVTV"
BASE COUNT          546 a         1131 c         1209 g          592 t
ORIGIN      
        1 gcccaccgcg cggcagccgg tggaggtcgt ggtcgaagtc gccgacgacc ggttcgacgt
       61 cgatgccggg atactcctcg gcgatcgcgg cggcggcgtc cttcaacgtc tgttcgctca
      121 cgtcgaacgg cacgaaccgc cgcagcgtgc cgtggtcgcg gagcgcgtcg agcaacaggc
      181 gcgtcttctc cgacgtcccc gaacccagct cgacgagcgt gcccgcatcc gtcgccgccg
      241 cgatctccgc cgcgcgcgcg acgaggatcg agcgttcggc gcgcgtcggg tagtactccg
      301 gtaggcgcgt gatctcgtcg aacaacgccg acccgcgatc gtcgtagaac cacttcggtg
      361 gcaggtcctt cggcgtcgac gtgagcccga cgcgcgcgtc gtccccgagc gtggtcacga
      421 cgcgtccctt gccaggcgca cgccgccgta catccacctg ctgccgggcg ggaagaagtt
      481 gcggtacgtc ggccgcacgt ggtcgggcga ggtgatgcgc gcgccgccgc gcaacaccat
      541 ctggcccgac atgaacttgc cgttgtactc accgacggcg ccggccgcgg gcgcgaaccg
      601 cggatacggg agatacgcgc tcgacgtcca ctgccacgcc tcgccgctga tctgcgcgag
      661 cgaggggacc gtcgataccg cgtgctccca ctcgaattcg gtcggcaggc gcgcgccgct
      721 ccaccgcgca aaagcgtccg cttcgtagtg actcacgtgg acgacgggtt cgccggggtg
      781 caacggtcgg cggccctgca gcgtgaacgc cgtccagtcg ccgtcgtggt cgtcccagta
      841 cagcggcgcg ttccagccgt gctcctgcac cgcgtaccag ccgtcgctga gccacagctc
      901 cgagcgcgtg tagccaccgt cggcgatgaa gtcgatccac tccgaggcgg tgaccagtcg
      961 gttcgcgatc gagaagccct cgacgagcac cgtgtgctgg ggcgtctcgt tgtcgaagca
     1021 gaacgtcgtg tcgccgtggc cgatcgtggc gaagccgccg tcgacgtcgt gccagcgcag
     1081 cgccggcgcg gtcacgggtt cgtcggcgtt cggcgcgtac gcgggttcga gcgggttgca
     1141 cgacagcacg tgcttgatgt ccatgaggag cagctcttgg tgctgttgct cgtgatgcag
     1201 cccgagctcg atcaacgcgc atacgtcggg ccagatcgcg tcggcgcaac gactgatcaa
     1261 gtcgtccatc atcacgtcga catgggcgcg gtatcgcgcg acctcttcga cggtcggtcg
     1321 cgacagcagg ccgcgcaacg ggcggggatg acgcggcccg actgcttcgt agtacgagtt
     1381 gaagaggtag acgaacgccg ggtgatacgg ctggtagcca tcgagcgagg gctggaggag
     1441 gaaggtttcg aagaaccagg tggtgtgccc gcggtgccac ttggtcgggc tcacgtcggg
     1501 catcgactgc acgacttggt cttcgggacc cagcggtgcg gccagggatt ccgtgaagtt
     1561 gcggatcgcg tgaaagcgag tgcgcagtgc ttcgcggtcg acgattgcgg tcgcggtggc
     1621 catcagtcgt tactgccgaa gcaagctgca acccacgcgt ggttgcgaac cggggaccac
     1681 cattcgatga cgacgaacat cgcaactcct ctactcgggg gagatgtccg gtgaaagggt
     1741 tacgtcacgg tacccctggg cggatgcgtc gcagtcgcag aattcagctg tgacgcagag
     1801 acgcgcgcag cgcggcgtgc acacccgcag gcgtgagcac gccgcaaaag tggtcatcgt
     1861 cgaggacggc gatccatccg tcgtcgctga gcagcgtgtg cgccaacgcg tgctcgaggg
     1921 tgtcgtggac gtcgacccac gcctcgacgc gggcgacacg ctcggcaacg gtgccctcgc
     1981 cgtccgcgtc gacgctgcgg atccggccgc acaggcgctg gtgatcgtcg acgatcggta
     2041 ctgccgagcc ctgttcgccc aggagttggc gggcgtcggc caggagcgcg tcgggccgca
     2101 gcgtcggcgg atgctcgagg tactcggtgt cgatcatcgt gaccttcagc tgcttgatcg
     2161 cgcggtcgat gccgacgaac gacgcgacga agtcggtcgc gggatgcccg aggatctcgg
     2221 acgggatgtc gtactgctcg aggtggccgc cgatgttcag gatcgcgatg cgatcaccga
     2281 gcttgattgc ctcttcgatg tcgtgggtga cgaacacgat cgtcttctgc aaccgctcct
     2341 ggagccgcac gaactcgtcc tgtaagcgtg cgcgcgtcac cggatcgatc gcaccgaagg
     2401 gttcgtccat caacagaacc ggaggatcgg ccgcgagtgc gcgcgcgacg ccgacccgct
     2461 gacgttgtcc accggagagt tgatgggggt agcgatcgcg gtattcgtcg gcgtcgagac
     2521 cgacgagtgt cagcaactcg tcggaccgtt cgcggatgcg cgcacgcgac cagccgagca
     2581 tgcgcggcac ggtgccgatg ttgcgctcga tcgtctggtg cgggaacaga ccgatctgct
     2641 ggatcacgta acccatgcgg cgccgcagct cgacgggatt ggtgtcggtg atgtcctcgt
     2701 cgtcgagata gatgcggccg ctcgtcgggt cgatgagccg gttgatcatg cgcagtagcg
     2761 tggtcttgcc gcaccccgac ggaccgacca gcaccgcggt ctcaccggtt ggaacggaga
     2821 gtgtcagctc gtgcactgca agcgtcccat ttgggtaacg cttgctgacc gcctcgagcc
     2881 ggatcatccg cgacgcagcc tacgcacgga acacgcccgg taatcgtccg agcgcggtcg
     2941 tatgatccgc cgacctctcg cgaggcgcag aggagctcct ggaaatgacc ggtgtgtcga
     3001 gcggtgtgct cgccgcgtac gacgggcgag cgtggatcga ctggacgtgg gtcggcgatc
     3061 acggcgacga gatcctggcg cgcttgcgcg agcacgccgc gctcacgttg tgggcgatgg
     3121 tgctcggcat cgtcatcgcg ttcccgctcg cgctcgtgtc ggtgcaccgg cggcgcacgc
     3181 gcaagttcgt gctcgcgacg acgggcatcc tctacacgat cccgtcgctc gccgcgttcg
     3241 cgctgctgtt gccctatacg ggcttgtcgc gcatgaccgc gatcattccg ctcgcgacgt
     3301 acacactgct catcctgatc cgcaacatcg tcgccgggct cgacgcggtg ccggtcgagg
     3361 tgcaagacgc cgccgtcggt atgggctatt cgagcacgcg caagatgctg cgcgtcgacc
     3421 tgccgctcgc gctgccgacg atcttcgcgg gcgtgcgcat cgcggcggtc acggtcat
//
LOCUS       DASUGA010000025         5077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_964407, whole genome shotgun sequence.
ACCESSION   DASUGA010000025 DASUGA010000000
VERSION     DASUGA010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964407"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VFX21_00480"
     CDS             <1..419
                     /locus_tag="VFX21_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017541070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094448.1"
                     /translation="MLIADRDFAEKHGLPIRARIKHFAVAAEDPVLVLSAPVPVTYKL
                     MERTGLKVDEFDAVECNEAFAAIALMWQKEFGYPMERFNPRGGAIALGHPLGASGVAL
                     MTRLLRHLEATNGTYGFQTMCEGGGQANATVIERLS"
     gene            complement(460..1305)
                     /locus_tag="VFX21_00485"
     CDS             complement(460..1305)
                     /locus_tag="VFX21_00485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX5094449.1"
                     /translation="MKLGIVTPVLTLLPRAHAAWEETGTLDDVVDIVQAAEALGYHHC
                     TCSEHVGVPVEVATTRGGRYWDPLATFGYLAARTTKIRLASHVLVLGYHHPLAIAKRY
                     GTLDVVSGGRLILGVGVGSLEEEFELLGAPFADRGARADDAMRALRAALSQPEPSYAG
                     EYYSFEGFLVDPCAVQERVPMWVGGRTFRSLRRAVELGDGWAPFGLSSVQLGEMLARV
                     DVPDGFDVVLQNEHPLDPAGEPDRVREQLEKFRAIGATACNVRFVHHSVEHYKDQLAA
                     LAALL"
     gene            complement(1416..2054)
                     /locus_tag="VFX21_00490"
     CDS             complement(1416..2054)
                     /locus_tag="VFX21_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5094450.1"
                     /translation="MVAQPVSPESGRRYRGLSQEERRADQRRRLVRAAIDEFAEHGYH
                     KTSVEDIVRRARTSRTAFYAFFDNREDAMYGALQTSMRALLDDVRRAIFRAGPGDDPV
                     AVAIRAYVEHLVADPAAATIVLLEGVGTSPEVNALRSRTRRELADLIRDMWAEYDPPA
                     ASQPSARAMSVGVLGVITESMVHLVETDRLSEGLEHVPALVNAVERMFSPRA"
     gene            2095..3759
                     /locus_tag="VFX21_00495"
     CDS             2095..3759
                     /locus_tag="VFX21_00495"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX5094451.1"
                     /translation="MSDKRTSLGDAVAQHVRAGDVLHPVVGHTRWSAATREVIRQWWG
                     RDPGFTLVMLSLSSLGALFFRGGLVRKVITGYSGDTFPNFTPNPWFAGAYERGEVDVE
                     HWSFLAFSQRLEAAARGLPAIVTRSIEGSSMAANDAYAQVDTPFGRAGVLAPLVPDVA
                     ILHAPVADCAGNVALHAPLLEGVWGALAARRGAIVTVERVVDDLRPWSHLVRIPAHRV
                     LAVVEAPMGAHPGGCYTGDLPVDGYGEDYEFWVDARTATRSDDYDAWIEHWVLDVTTQ
                     DEWLARVGRERVQALRAKADPDSWRADERRLPVDESSPPNAWERAAVWGARYLADRVI
                     ARDANAVLAGAGVANLAAWLGVRIARERGADVQLTAEIGLWGYDATPADPFVLNHRNF
                     PTATMLSDAQTVLGSLVGGTGTTTIGCLGGAQIDRFGNINSTWIPPRPFLVGSGGGND
                     VASTAAENVVVATMTKPRTPPDCGYITSPGRTVRALVTDLGILEKDGDELVLTAVAPG
                     VSVDDVRASCGWELRRAGDIAELTLPEHEEVLALRRWDPQGWFLRA"
     gene            3792..4802
                     /locus_tag="VFX21_00500"
     CDS             3792..4802
                     /locus_tag="VFX21_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5094452.1"
                     /translation="MARVVFDHVTKRFGDVVAVRELDLEVRDREFLVLLGPSGCGKST
                     ALRMIAGLEDPSEGDIIIGDDVVTDVEPKDRDVAMVFQSYALDPHMTVARNIEFPLRT
                     RSVAPADRAKAVQEAADALGLTDLLARKPAQLSGGQRQRVALARAIVRQPKAFLMDEP
                     LSNLDAKLRVQTRAELVELQRRLDATVVYVTHDQIEAMTMGHRIAIMSMGELQQVGPP
                     SEVYEKPANLFVASFIGTPPMNIFDGELLGRSAAGMVGVRPEHMRFDGNGPLAATVTA
                     IESLGHERHVACRLADGTLVIVRQGSNDDAPAVDSSAQLAIAPDRLHCFDPATGRRLE
                     TV"
     gene            4799..>5077
                     /locus_tag="VFX21_00505"
     CDS             4799..>5077
                     /locus_tag="VFX21_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX5094453.1"
                     /translation="MNRRTREALLGYLFILPSMIIFGTFVFYPFVKNFWLGLYKTPPF
                     PGLPRKWAGIEQYKDVLTSSDFYNSLKVTLLFVLFTVPVGIVLGLALAV"
BASE COUNT          782 a         1734 c         1748 g          813 t
ORIGIN      
        1 cgatgctcat cgccgatcgc gacttcgccg agaagcacgg cctgccgatc cgcgcccgca
       61 tcaagcactt cgccgtcgcg gccgaagacc cggtgctcgt cctctcggcg ccggtgcccg
      121 tcacctacaa gctgatggag cgcaccggcc tgaaggtcga cgagttcgac gcggtcgagt
      181 gcaacgaggc cttcgccgcc atcgcgctca tgtggcagaa ggaattcggc tacccgatgg
      241 agcgcttcaa cccccgcggc ggcgcgatcg cgctcggcca ccccttgggc gcgtccggcg
      301 tcgcgctgat gacgcgcctg ctccgccacc tggaagcaac gaacggcacc tacggcttcc
      361 agaccatgtg cgaaggcggc ggccaagcca acgccacagt catcgaacgc ctgtcttgac
      421 gcttcttgtg atccagatgg ccatatatgg ccgtctggat cacaacaatg cggcaagggc
      481 ggcgagttgg tccttgtagt gctcgacgga gtggtggacg aacctgacgt tgcacgcggt
      541 ggcgccgatg gcgcggaact tctcgagctg ctcgcggaca cggtcgggtt cgccggcggg
      601 gtcgagtggg tgttcgttct gcaggacgac gtcgaatccg tcgggcacgt cgacgcgcgc
      661 cagcatctcg ccgagctgca cactgctcaa cccgaacggc gcccaaccat caccgagctc
      721 gaccgcgcgg cgtagcgaac ggaacgtgcg gccgccgacc cacatcggca cgcgctcctg
      781 caccgcgcac gggtccacga ggaagccctc gaacgagtag tactcgcccg cgtacgacgg
      841 ctcgggttgc gacagcgcgg ctcgcagcgc gcgcatcgcg tcgtcggctc gggcgccgcg
      901 gtcggcgaac ggtgcaccga gcagctcgaa ctcctcctcc aacgagccca cgcccacgcc
      961 gaggatcagc cggccgccgg agacgacgtc gagcgtgccg tagcgcttcg cgatcgccaa
     1021 cgggtggtgg tagccgagca cgagcacgtg gctggcgagg cggatcttcg tcgtgcgcgc
     1081 cgcgaggtac ccgaacgtcg cgagcgggtc ccagtaccgg ccgccccgcg tggtcgcgac
     1141 ctcgaccggc acgccgacgt gctcgctgca cgtgcagtgg tgatagccga gcgcttcggc
     1201 cgcttgcacg atgtcgacga cgtcgtcgag cgtgcccgtc tcttcccatg cggcgtgcgc
     1261 gcgcggcaac agcgtgagca caggggtgac gatgccgagc ttcatgacgc gaagtggccg
     1321 cgggccggca gtagtggggg taccgccgac ctcgcggccg tcgggatgat cgcgcggtcg
     1381 gagggcgcgg tcacagctcg cgacttattc gcgattcacg cgcgcggaga gaacatgcgc
     1441 tcaaccgcat tgacgagcgc cggcacgtgc tcgagcccct ccgacaatcg gtccgtttcg
     1501 acgagatgca ccattgactc ggtgatgacg ccgagtacgc cgaccgacat cgcgcgtgca
     1561 ctgggctggg aggcggcagg agggtcgtac tcggcccaca tgtcgcggat caggtcggcg
     1621 agctcccgcc gagtgcgtga ccgcaacgcg ttgacctctg gcgaggtgcc gacgccttcg
     1681 agcaggacga tcgtcgcggc cgccgggtct gcgaccaggt gttctacgta cgcgcgaatg
     1741 gccaccgcga ccgggtcgtc gccgggaccg gcgcggaaga tcgcgcgccg gacgtcgtcg
     1801 agcagcgcgc gcatcgacgt ctggagcgcg ccgtacatcg catcttcccg attgtcgaag
     1861 aacgcgtaga acgcggtacg gctcgtccgc gcgcgccgca cgatgtcttc gacggaggtc
     1921 ttgtggtaac cgtgctcggc gaactcgtcg atcgcggcgc gcacgaggcg gcggcgctgg
     1981 tcggcgcggc gctcttcttg tgacagaccg cggtagcgac gaccgctctc aggactcacg
     2041 ggctgggcaa ccacgacatc aggttagcgg cggtacaaca ggtaggtttc cggcgtgtca
     2101 gacaagcgga ccagcctggg tgacgcggtt gcacagcacg tgcgcgcggg tgacgtgctg
     2161 catcccgtcg tcggtcacac gcgctggagc gcggcgacgc gcgaagtgat ccgccagtgg
     2221 tggggccgcg acccgggctt caccctcgtg atgctctcgc tctcgagcct gggtgcgctg
     2281 ttcttccgcg gcggtctggt gcgcaaggtg atcaccggat actcaggtga cacatttccc
     2341 aacttcacgc ccaacccttg gttcgcgggc gcgtacgagc ggggcgaggt cgacgtcgag
     2401 cattggtcgt tcctcgcgtt ctcgcagcgg ctcgaggcgg cggcccgcgg gttgccggcg
     2461 atcgtgacgc ggtcgatcga aggctcgtcg atggcggcca acgacgccta cgcccaggtc
     2521 gacaccccgt tcgggcgcgc cggcgtgctc gcgccgcttg tcccggatgt cgcaatcctg
     2581 catgcaccgg tcgccgattg cgccggcaac gtcgcgttgc acgccccgct gctcgaaggt
     2641 gtctggggcg cgctcgcggc tcggcggggt gcgatcgtga ccgtcgaacg cgtcgtcgac
     2701 gacctgcgcc cgtggtcgca cctcgtccgc attcccgcgc accgcgtcct cgccgtcgtc
     2761 gaagcgccga tgggtgcgca tcccggcggt tgttacacgg gcgatctccc cgtcgacgga
     2821 tacggcgagg actacgagtt ctgggtcgac gcgcgcaccg cgacccgcag cgacgactac
     2881 gacgcgtgga tcgagcactg ggtgctcgac gtgacgacgc aggacgaatg gttggcgcgc
     2941 gtcggtcgag aacgcgtgca ggcactgcgc gccaaggcgg accccgactc gtggcgcgcc
     3001 gacgagcggc ggctcccggt cgacgagagc tcgccgccga acgcgtggga gcgcgccgcg
     3061 gtctggggcg cgcgctatct cgccgatcgg gtcatcgcgc gtgacgcgaa cgcagtgctc
     3121 gctggcgcgg gtgtggcaaa cctcgcggcg tggctcggcg ttcggatcgc gcgcgaacgc
     3181 ggcgccgacg tgcaactcac ggccgagatc ggattgtggg ggtacgacgc gacgccggcc
     3241 gatccgttcg tgctcaacca ccgcaacttc ccgaccgcga cgatgttgag cgacgcgcag
     3301 acggtgctgg gctcgctcgt cggtggcacc ggcaccacga cgatcggctg cctgggcggc
     3361 gcgcagatcg accggttcgg caacatcaac tcgacgtgga tcccgccgcg tccgttcctc
     3421 gtcggttccg gcggcggcaa cgacgtcgcg agcacggcgg ccgagaacgt cgtcgtcgcg
     3481 acgatgacca agccgcgcac accacccgac tgcggataca tcacgtcgcc cgggcgcacg
     3541 gtgcgcgcgc tcgtcaccga cctcggcatc ttggagaagg acggcgacga gctcgtactc
     3601 accgcggttg cacccggcgt cagcgtcgac gacgtccgcg cgtcgtgtgg ctgggaactg
     3661 cgcagagcgg gcgatatcgc cgagctgacg ttgccggaac acgaagaagt ccttgcgctt
     3721 cggcgctggg acccgcaggg ctggttcttg cgcgcgtaac gccgcggttg ggtcgttatg
     3781 ctcccgccgc aatggcacgt gtcgtcttcg atcatgtgac caagagattc ggcgacgtcg
     3841 tcgcggtgcg cgagctcgat ctcgaggtgc gcgaccgcga gttcctcgtg ttgctcgggc
     3901 cgtcgggctg cgggaagtcg acggcgctcc ggatgatcgc cggtctcgaa gatccttcgg
     3961 aaggcgacat catcatcggc gacgacgtcg tcaccgacgt cgaaccgaag gatcgcgacg
     4021 tcgcgatggt gttccagagc tacgcgctcg acccgcacat gactgtcgcc aggaacatcg
     4081 agttcccgtt gcgcacccgc agcgttgcgc cggccgatcg ggccaaggcg gtgcaagagg
     4141 ccgccgacgc gctcggcctc accgacttgc tcgcgcgcaa gcccgctcaa ctctccggtg
     4201 gacagcgcca gcgcgtcgcg ttggcgcgcg ccatcgtgcg ccagccgaag gcgttcttga
     4261 tggacgagcc gctctcgaac ctcgacgcga agctgcgggt gcagacccgc gccgagctgg
     4321 tcgagctgca gcggcgtctc gatgccaccg tcgtctacgt cacccatgac cagatcgaag
     4381 cgatgacgat gggtcaccgc atcgcgatca tgagcatggg cgagctgcaa caggtcggtc
     4441 cgccgagcga ggtgtacgag aagccggcga atctcttcgt cgcatcgttc atcggtacgc
     4501 cgccgatgaa catcttcgac ggtgagctcc tcggccggtc cgcggcgggc atggtcggtg
     4561 tccggcccga gcacatgcgc ttcgacggca acggcccgtt ggcggccacg gtcaccgcga
     4621 tcgagtcgct cggtcacgaa cggcacgtcg cgtgccgcct cgccgacggc acgctcgtca
     4681 tcgtgcgcca gggaagcaat gacgacgcgc cggcggtcga ctcgtccgcg cagctcgcga
     4741 tcgcacccga ccgcttgcac tgcttcgatc cggcgacggg ccgacgcctg gagacggtgt
     4801 gaaccgccgg acgcgcgaag ccctgctcgg ctacctgttc attctcccgt cgatgatcat
     4861 cttcgggacg ttcgtcttct acccgttcgt caagaacttc tggctcgggc tctacaagac
     4921 gccgccgttc cccggtctgc cgcgcaagtg ggccggcatc gagcagtaca aggacgtgct
     4981 cacgtcgagc gacttctaca acagcctcaa ggtcacgttg ctgttcgtgt tgttcaccgt
     5041 gcccgtcggc atcgtcctcg gtctcgcatt ggcggtc
//
LOCUS       DASUGA010000026         5378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1102749, whole genome shotgun sequence.
ACCESSION   DASUGA010000026 DASUGA010000000
VERSION     DASUGA010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102749"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..585)
                     /locus_tag="VFX21_00510"
     CDS             complement(148..585)
                     /locus_tag="VFX21_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HEX5094454.1"
                     /translation="MTRPDLDRRDRIEQFVRDFYRQVAMDDLLGPVFARAHVDWSAHL
                     PKMVDFWSWQLLGEARYERNPLRAHERVHAQDPFTPALYERWLELFDGTVDDGFAGPL
                     ADVAKQRARRMAQALRRLLDGTSAPGTEPVEPVAPTLLARAAT"
     gene            complement(582..986)
                     /locus_tag="VFX21_00515"
     CDS             complement(582..986)
                     /locus_tag="VFX21_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX5094455.1"
                     /translation="MRLEVTRRADLAVRTVAMLAHGRRVKAPALAAALGTTAGFVPQV
                     VGPLVKAGWIVSEPGPTGGYRLAIPVESLSVLDVVEAVDGPTDTGRCVVADRPCGRGK
                     ECALHDAWARARRELIASLGQMAVPDTEALAK"
     gene            1108..2103
                     /locus_tag="VFX21_00520"
     CDS             1108..2103
                     /locus_tag="VFX21_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5094456.1"
                     /translation="MAVLIALAIAILGSGELRWFDAALVGYLFGTLFAIFAVVYRYLV
                     WLQRPPTARLNRRGWEAFRARGRRTRNAAALPVLLVSNLLLQSFIRGRSRARWFAHQL
                     LFWGCVLAALVTFPLVFGLLHFESVGQNGREYRTVIGEISTFSFDSRSVFGWIVFHLL
                     DISAVLVLAGVFIFLARRLRDPGALAVERANDFLPLAGLFAVSVTGLALTASNLWMEG
                     RFYVFLNTVHALTVILGLMYIPFGKLFHIFQRPANIGVEYYRREGAEGVQQHCARCSQ
                     PFAAQLQMIDLKEVLPQVGFDYSIAGDETNYQDHCPRCRRQLVALAQSRAVGGFG"
     gene            2103..4388
                     /locus_tag="VFX21_00525"
     CDS             2103..4388
                     /locus_tag="VFX21_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEX5094457.1"
                     /translation="MARPPVSEEDLVARFGPHLNQAPPGGWNAGVDADREVKTHCCFC
                     GQQCGIILKVKGEEVVGFEPWYEFPFNEGKLCPKGVKRYLQNAHPDRLLDPLERDPAH
                     PGGFRPVSWDHALDRTVSEIRRIQSTYGNDAFAMLSGVSLDNEKSYMIGKFARLALGT
                     ANLDYNGRLCMVSAGVANKRALGIDRATNPWSDIPLAEVVFVIGANIAECAPITTNWI
                     WRARDNGAKLIVADPRVTPTARTADLFLGLRPGTDSALLGAILHVLIERDLLDHEFIR
                     EHTSGFDEAAAAVRDHTPQWAEQVTGVPAARIEQAAEWWGSARTGMLLHARGLEHHTK
                     GVENVWACINLGLATGKYGKPGCGVTTITGQGNGQGGREHGHKCDQLPGNRDITNPEH
                     RAYVASVWGCDVDEIPGKGLTAEEIVEAIHRGEIKGLLSICFNPVVSLPDTAFTKEAL
                     DRLEYYALIDFFMPETGYHADVVMPGSLHEEDEGTSTTLEGRVVKLNPSKKPPGNARL
                     DWEILCDIAARLGRGKYFPYRDAEEIFEELRVASKGGSADYAGITWERIERELGVFWP
                     CPSEDHPGTKRLYEGGRFHTDDGRGRFNPVRYRPPAEVVDDEYPVWLTTGRVVSQYLS
                     GTQTRRIGPLVAQYPEPLCELHPRLAEQYGINTGDAMRVTSRRGTMTLPAKVVETIRP
                     DTIFIPYHWAGRRAANQLTNRALDPLSKIPEFKVCAVRIERVGPPGSVVVDERDMDLV
                     ERDTRGDEPRGPASEPGEGGG"
     gene            4385..5068
                     /locus_tag="VFX21_00530"
     CDS             4385..5068
                     /locus_tag="VFX21_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5094458.1"
                     /translation="MSGTSVPVTIGVKRTPATRPIAGAFGDNIFVIDQSRCIGCQACV
                     QACEECGTHRGNSMIHLDEIDRYATTQTAPMVCMHCEDPTCAEVCPADAIKQNEDGVV
                     QSSLKPRCIGCSNCVLACPFGVPKYFSAYDQMMKCDMCYDRTSVGLKPMCASVCPSEA
                     LWFGTPDEFAQHRTGVLVNEWWFGRQYVATKVRTVADSAGSIDVLAAGDARPWQQDPF
                     GLDDTASER"
     gene            5065..>5378
                     /locus_tag="VFX21_00535"
     CDS             5065..>5378
                     /locus_tag="VFX21_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094459.1"
                     /translation="MSHEVPVPAPEPVWRRDFPLTSTGEDEVTRREFVRYLVLASAGF
                     AAGNVGIALWSSLHSINEGEPRPIIALDRLPEGTAHLFDYPTDADPAILVHLPGGELR
                     AF"
BASE COUNT          869 a         1855 c         1814 g          840 t
ORIGIN      
        1 gtcacgctca ccggctggtc acgtcctctc ggccagttgc ccgcaatccg ccgggcaccc
       61 aaatacccgg gctcatggtg cgcaacaggc cgagcaccgg atacacccga ttcgggtggc
      121 tggagcgtga gctcgggagg cgaacttcta cgttgccgcc cgggccagca acgtcggcgc
      181 aacgggctcg acaggctcgg tgccgggcgc gctggttccg tcgagcagcc gtcgcagcgc
      241 ctgcgccatg cggcgggccc gctgtttggc aacgtcggca agcggccccg caaagccgtc
      301 gtcgacggtc ccgtcgaaca gttcgagcca gcgttcgtac agcgcgggtg tgaacggatc
      361 ctgggcgtgg acgcgctcgt gcgcgcggag cgggtttcgc tcgtagcgcg cttcgccgag
      421 cagctgccat gaccagaagt cgaccatctt ggggagatgc gcggaccagt cgacgtgagc
      481 gcgcgcgaag acgggcccga gcaggtcgtc catcgcgacc tgtcggtaga agtcgcgtac
      541 gaactgctcg attcgatcgc gccggtcgag gtcgggccgc gtcacttcgc gagcgcttcc
      601 gtgtcgggga ccgccatctg gccgagcgac gcgatgagct ctcttcgcgc acgcgcccac
      661 gcgtcgtgca acgcgcactc cttcccgcgg ccacagggac ggtcggcaac gacgcagcgc
      721 ccggtgtcgg tcgggccgtc gacagcctcc accacgtcga ggacggacag cgattcgacc
      781 ggtatcgcga gccggtagcc acccgtcggc ccgggttcgg agacgatcca accggccttg
      841 acgaggggac cgacgacctg cggcacgaac ccggccgtcg tcccgagcgc ggcggccagc
      901 gccggcgcct tgactcgccg tccgtgtgcg agcatcgcga cggtccgcac cgccagatcc
      961 gctcggcgcg tgacttcgag tctcatagtg aaagactata ggattcgcgg tcgggcaccg
     1021 aagccacgtt ccgggtagga gcacagcctg atggcgctcg agcccagcgc tccgagaatt
     1081 gaccggcgtt ccgcgacgtg gggcgccgtg gccgtgctga tcgcactcgc gatcgcgatc
     1141 ctcgggagcg gtgagctgcg gtggttcgac gccgcgctcg tcggctacct gttcggcacg
     1201 ctgttcgcga tcttcgcggt cgtctaccgg tatctcgtct ggttgcagcg accaccgacc
     1261 gcgcgtctca accggcgggg gtgggaggcg ttccgcgcgc gcggccgtcg cacgcgcaac
     1321 gccgcggcac tcccggtctt gctcgtgagc aacctgttgt tgcagtcgtt catccgcggg
     1381 cggtcccgcg cccggtggtt cgcgcaccag ttgctcttct ggggctgcgt gctcgcggcg
     1441 ctggtgacgt tcccgctcgt gttcggactg ttgcacttcg aatcggtcgg tcagaacggg
     1501 cgcgagtacc gaaccgtcat cggcgagatc tcgacgttca gcttcgacag ccgcagcgtg
     1561 ttcggctgga tcgtgttcca tctgctcgac atctccgccg tcctcgtgtt ggcgggcgtc
     1621 ttcatcttcc tggcccggcg cctgcgcgat ccgggcgcgc tcgcggtgga gcgcgccaac
     1681 gacttcttgc cgttggccgg attgttcgca gtgtcggtga cgggcctcgc gttgaccgcg
     1741 tcgaacctgt ggatggaggg ccggttctac gtgttcctca acaccgtgca cgcgctgacg
     1801 gtgatcctcg gcctgatgta catcccgttc ggcaagttgt tccacatctt ccaacgcccc
     1861 gcgaacatcg gcgtcgagta ctaccgccgc gaaggcgcag agggcgtgca acagcactgt
     1921 gcgcggtgct cgcagccgtt tgccgcgcag ctccagatga tcgacctcaa ggaggtgctc
     1981 ccgcaagtgg gcttcgacta ctcgatcgcc ggtgacgaga cgaactacca ggaccactgc
     2041 ccgcgctgcc gccggcagtt ggtcgcgctg gcacagtcgc gcgcggtcgg cgggttcggc
     2101 tgatggcgcg cccaccggtc tccgaagaag acctcgtcgc gcgcttcggg ccccatctga
     2161 accaggcgcc gcccggcgga tggaacgccg gcgtcgatgc cgaccgcgag gtgaagacgc
     2221 actgctgctt ctgcggccaa cagtgcggca tcatcctcaa ggtcaaaggc gaagaggtcg
     2281 tcgggttcga gccgtggtac gagttcccct tcaacgaggg caagctgtgc ccgaagggcg
     2341 tcaagcggta tctccagaac gcgcaccccg accggctcct cgatcccctc gaacgtgacc
     2401 ccgcacatcc cggcggattc cggcccgtgt cgtgggacca cgcgctcgac cgcaccgtct
     2461 ccgagatccg gcgcatccag tccacctacg gcaacgacgc gttcgcgatg ttgtcgggcg
     2521 tctcgctcga caacgagaag tcgtacatga tcggcaagtt cgctcgcctc gcgctcggca
     2581 cggccaacct cgactacaac ggccgcctct gcatggtctc ggccggtgtc gccaacaagc
     2641 gagcgctcgg tatcgaccgc gccacgaacc cgtggagcga catcccgctc gccgaagtcg
     2701 tgttcgtgat cggggcgaac atcgccgagt gcgcgccgat cacgacgaac tggatctggc
     2761 gggcgcgcga caacggcgcg aagttgatcg tcgccgatcc gcgtgtgacg ccgaccgcgc
     2821 gcaccgccga cctcttcctc ggactccgac cgggaaccga ttccgcgctg ctgggtgcga
     2881 tcctgcacgt gctgatcgag cgggatctgc tcgatcacga gttcatccgc gagcacacga
     2941 gcggattcga cgaggcggca gccgcggttc gtgaccacac gccgcaatgg gccgagcagg
     3001 tgaccggcgt gcccgcggcg cgcatcgaac aggcggccga atggtggggg agcgcgcgca
     3061 ccgggatgct gctgcacgcg cgcgggctcg agcaccacac caagggcgtc gagaacgtct
     3121 gggcgtgcat caacctgggg ctcgccaccg ggaagtacgg caagccgggg tgcggcgtca
     3181 ccacgatcac ggggcaaggc aacggccagg gcgggcgcga gcacgggcac aagtgcgacc
     3241 agctcccggg caaccgcgac atcacgaacc ccgagcaccg cgcgtacgtc gcctcggtgt
     3301 ggggctgcga cgtcgacgag attcccggga agggcctgac cgcggaggag atcgtggaag
     3361 cgatccaccg cggcgagatc aaggggctgc tgtcgatctg cttcaacccg gtggtgtcac
     3421 tccccgacac cgcgttcacg aaggaagcgc tcgaccggct cgagtactac gcactcatcg
     3481 acttcttcat gcccgagacc ggataccacg cggacgtcgt catgcccggc tcgctgcacg
     3541 aagaagacga aggcacctcg acgaccctcg aggggcgcgt ggtcaagctc aacccgtcga
     3601 agaagccacc cggcaacgcg cgcctcgact gggagatcct ctgcgacatc gccgcgcgcc
     3661 tcgggcgggg caagtacttc ccgtaccgcg acgcggagga gatcttcgag gaactgcgcg
     3721 tcgcgtccaa aggcggcagt gcggactacg ccggcatcac gtgggagcgc atcgagcgcg
     3781 agctcggtgt gttctggccg tgcccgtccg aagaccatcc gggcacgaag cgtctctacg
     3841 agggcggccg cttccacacc gacgacgggc gcggccgctt caaccccgtg cgctaccgcc
     3901 cgccggcgga agtggtcgac gacgagtatc cggtctggct cacgacgggc cgcgtcgtca
     3961 gccagtacct ctcggggacg cagacgcgcc gtatcggtcc actcgtcgcg cagtatccgg
     4021 agccgttgtg cgagctgcat ccgcgactcg ccgagcaata cggcatcaac accggcgacg
     4081 cgatgcgcgt gacgtcgcgg cgcgggacca tgacgctgcc ggccaaggtc gtcgagacga
     4141 tccggcccga cacgatcttc atcccgtacc actgggccgg ccggcgggcc gcgaaccagc
     4201 tcacgaaccg agcgctcgac ccgctctcga agatccccga attcaaagtg tgcgccgtgc
     4261 gcatcgaacg cgtcggtccg ccgggtagcg tcgtggtcga cgagcgcgac atggatctcg
     4321 tcgagcgcga cacgcgcggc gacgaacctc gcgggccggc gtccgagccg ggggagggcg
     4381 gcggatgagc ggcacgagcg ttcccgtgac catcggcgtg aagcgcacgc ccgcgacgcg
     4441 accgatcgcc ggcgcgttcg gcgacaacat cttcgtcatc gaccagagcc gttgcatcgg
     4501 ctgtcaagca tgcgtgcagg cgtgcgaaga gtgcgggacg caccgcggca actcgatgat
     4561 ccacctcgac gagatcgatc gctacgcgac gacacagacc gcacccatgg tctgcatgca
     4621 ctgcgaggat ccgacgtgcg cggaggtgtg ccccgcggat gccatcaagc agaacgaaga
     4681 cggtgtcgtc cagtcttcgc tgaagccgcg gtgcatcggc tgttccaact gcgtgctcgc
     4741 gtgcccgttc ggcgtgccga agtacttctc cgcatacgac cagatgatga aatgcgacat
     4801 gtgctacgac cgcacctcgg tcgggttgaa gccgatgtgc gcgtcggtgt gcccgagcga
     4861 ggcactgtgg ttcggcacgc ccgacgagtt cgcgcagcac cggacgggag tgctcgtcaa
     4921 cgaatggtgg ttcggccggc agtacgtcgc gacgaaggta cggaccgtcg cggactcggc
     4981 cggctcgatc gacgtgctcg ccgcgggcga cgcccggccg tggcagcagg atccgttcgg
     5041 tctcgacgac acggcgagcg agcgatgagc cacgaggtgc cggtccccgc tccggagccg
     5101 gtctggcgcc gcgacttccc gctcacctcc accggcgaag acgaagtgac gcgtcgcgag
     5161 ttcgtccgct acctcgtgct ggcatccgcc ggcttcgcgg cgggcaatgt cggcatcgca
     5221 ctgtggtcgt cgttgcactc gatcaacgaa ggcgagccgc gcccgatcat cgcactcgat
     5281 cggctccccg aaggcaccgc ccatctgttc gactacccga ccgacgcgga ccccgcgatc
     5341 ctcgtccacc tgcccggcgg cgaactgcgc gccttcag
//
LOCUS       DASUGA010000027         4291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1240942, whole genome shotgun sequence.
ACCESSION   DASUGA010000027 DASUGA010000000
VERSION     DASUGA010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240942"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1018)
                     /locus_tag="VFX21_00540"
     CDS             complement(65..1018)
                     /locus_tag="VFX21_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX5094460.1"
                     /translation="MTLTAPAPVEAGEVTKSATTTETVGVRKGGWFTRITILLVVLLW
                     LIPVVGVLVTSFRSADLANSTGWWTAIMHPFRDGEWTLENYRTVLDAQGFENAFLNSI
                     AVAVPSTVIPITIAAFAAYAFSWMQFRGRYLMFVLVVGLMVVPLQMALIPILRLYTQG
                     ATIGGVQLIPDLDLNGTFFGVWLAHTAFGLPLAVYLLRNYIGALPSSIIESARMDGAD
                     HFTIFWRLIIPLSVPALAAFAIFQFLWVWNDLLVAYIFLGGTRQNRVLTIALANLNGA
                     HGEDWYLLTAAAFLTMILPLMVFFGLQRYFVRGLTAGSVKG"
     gene            complement(1015..2127)
                     /locus_tag="VFX21_00545"
     CDS             complement(1015..2127)
                     /locus_tag="VFX21_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006098732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX5094461.1"
                     /translation="MSTAAPATGRRFDARTVAGAIVTVAIPAIAVLLFWSTFDFLRDA
                     DANRFVVVGVALVVGVGGVFAMYWGTNRFVDWLPASFRETVRPYVFVGPALLILAIFL
                     IYPVINTIIISFKDARGNEFVGFDNYKFVFTDESMKRAIRNTLLWVVLVPGVAVTVGL
                     VFATLADRLARGEAIAKSLIFLPMAISFVGAAVTFRLIYSFRPEGFGSNIGLLNAIWT
                     GLGNNPVPWLSKQPWNNLLLMVIMIWMQTGFAMVVLSAAIKSIPDEIIEAARIDGASE
                     LQVFRRIIVPSIGPTIAVVTTYMVINALKVFDIVYVMGNPQANHTEVIAERMIRWFFI
                     SNHDGRGAAIAVVLFLAIIPVMIWNVRRFREQEMIR"
     gene            complement(2155..3339)
                     /locus_tag="VFX21_00550"
     CDS             complement(2155..3339)
                     /locus_tag="VFX21_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5094462.1"
                     /translation="MSAGEPEEVDAYQTIFDDLINSKTDYNVEVESVGDFEEQFQIRA
                     EGGTLDVAAAPQPGSIPALVDSGSIVSLEDLGFDIQELNDLVGESFVALGEYNGKHYG
                     IPTNINLKSMIWYPKDDFDAAGYQVPKTFDDLIALSDQIVADGSTPWCVGFGSEGSTG
                     WPATDWMEDIMLRTAGPEVYDQWVAHEIPFNDDAVRTAGETFGKVMFTDGYVLGGAAA
                     TPDIAFGDAPLPMFDTPPKCWLHRQASFINASFPETAQAGVDYDWFAFPPIDQDGTLY
                     AGELTVVGKNAARPEVVDFLKRFIAEDVQCEMGGVQASSRISPNVNVGADCYANDILA
                     DASVILTDSLKSGTGRFDASDLMPAEVGQGTFWTGMVDYMKGGPDSLNGVLDDIENSW
                     PS"
     gene            complement(3542..>4291)
                     /locus_tag="VFX21_00555"
     CDS             complement(3542..>4291)
                     /locus_tag="VFX21_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX5094463.1"
                     /translation="FLMDEPLSNLDAKLRVQMRAEIARLQHELGVTTIYVTHDQVEAM
                     TMGDRVAVLRRGRLQQVDNPQTLYEHPVNIFVGGFIGSPAMNLFDATLRRTGGRFAVV
                     FGGFCVSLPDETMAAHADLEGYEDQVIVLGIRPEDMEDAALVSDAPADRRIRSTVELR
                     ESLGSDVVVHFTIDAPPAMAEDAVELAVEVGTEALEQVQAQAQGGRSTMVARVDRRTA
                     VRKGDSIELVVDAQRLHFFDPKDGLGIYGGS"
BASE COUNT          788 a         1378 c         1417 g          708 t
ORIGIN      
        1 agcgttccga tcgcgatcgc gcgccacttg cgaggcgccg agtcggtggc aacgccgacg
       61 gccatcagcc cttcaccgat ccggcggtca ggccgcgtac gaagtagcgc tgcaatccga
      121 agaagaccat caacggcagg atcatcgtca agaaggcggc ggcagtgagc aggtaccagt
      181 cctctccgtg cgcaccgttg agattcgcga gggcgatggt cagcacgcgg ttctgacgcg
      241 tgccgccgag gaagatgtag gcgacgagga ggtcgttcca cacccagagg aactggaaga
      301 tggcgaacgc cgcgagcgcg ggaaccgaca gcgggatgat cagccgccag aagatcgtga
      361 agtggtcggc gccgtccatg cgcgccgact cgatgatcga cgacggcagc gcgccgatgt
      421 agttgcgcaa caggtacacc gcgagcggca acccgaaggc ggtgtgcgcc agccagacgc
      481 cgaagaacgt gccgttgagg tcgaggtccg ggatgagctg gacgccgccg atcgtggcgc
      541 cctgcgtgta gagccgcagg atcgggatga gcgccatctg gagcgggacg accatgaggc
      601 cgacgacgag cacgaacatg aggtaccggc cgcggaactg catccaggag aacgcgtagg
      661 ccgcgaacgc ggcgatggtg atcgggatga ccgtcgacgg caccgcgacc gcgatgctgt
      721 tgaggaacgc gttctcgaag ccctgcgcgt cgagcacggt gcggtagttc tcgagcgtcc
      781 attcaccgtc gcggaacggg tgcatgatcg cggtccacca cccggtcgag ttcgcgagat
      841 cggcggaccg gaacgacgtg acgagcacgc cgaccacggg gatcagccag agcagcacga
      901 cgagcaggat cgtgatgcgc gtgaaccagc cgcccttccg cacaccgact gtctcggtcg
      961 tcgtcgccga cttcgtcacc tcgccggctt cgaccggcgc gggtgcggtg agcgtcatcg
     1021 gatcatctcc tgctcccgga aacgccggac gttccagatc atgacgggga tgatcgcgag
     1081 gaagagcacg accgcgatcg ccgcaccgcg accgtcgtgg ttcgagatga agaaccatct
     1141 gatcatccgt tcggcgatga cctcggtgtg gttcgcctgg ggattgccca tcacgtacac
     1201 gatgtcgaag accttcagcg cgttgatcac catgtaggtg gtgaccacgg cgatggtcgg
     1261 gccgatgctc ggcacgatga tgcgccggaa gacctggagc tccgaggcac cgtcgatgcg
     1321 cgccgcctcg atgatctcgt cggggatcga cttgatggca gcggacagca ccaccatcgc
     1381 gaagccggtc tgcatccaga tcatgatgac catgagcagg aggttgttcc acggttgctt
     1441 cgacagccag ggcaccgggt tgttgccgag accggtccag atcgcgttca gcagcccgat
     1501 gttggaaccg aagccttcgg gccggaagct gtagatgagg cggaacgtca ctgccgcacc
     1561 gacgaacgag atcgccatcg gcaggaagat cagggacttc gcgatcgctt cgccgcgcgc
     1621 caaccggtcg gcgagtgtcg cgaagacgag gccgacggtg actgcgacgc cggggacgag
     1681 cacgacccac aacagtgtgt tgcggatcgc tcgcttcatg ctctcgtcgg tgaagacgaa
     1741 cttgtagttg tcgaacccga cgaactcgtt gccgcgtgcg tccttgaaac tgatgatgat
     1801 cgtgttgatc accggataga tcaagaagat cgccaggatc aacagcgcgg gtccgacgaa
     1861 cacgtagggc cggaccgtct cgcggaagct ggccggcagc cagtcgacga accggttcgt
     1921 gccccagtac atcgcgaaca cgccgcccac cccgacgacg agcgcgacgc cgacgaccac
     1981 gaaccggttt gcgtccgcgt cgcgcaggaa gtcgaacgtc gaccagaaca acaagaccgc
     2041 aatcgcgggg atcgcgaccg tcacgatcgc gcccgcgacc gtccgcgcgt cgaaacgccg
     2101 tccggtcgcg ggcgcagccg tgctcatgcc gtcgtgccta gaagatgggt gcgactacga
     2161 cggccaactg ttctcgatgt cgtcgaggac cccgttgagc gagtcggggc ctcccttcat
     2221 gtagtcgacc atgccggtcc agaaggttcc ctggccgacc tcggccggca tcaggtccga
     2281 ggcgtcgaag cggcccgtgc ccgacttcaa cgagtcggtc aggatcaccg atgcgtcggc
     2341 gaggatgtcg ttcgcgtagc agtcggcacc gacgttgacg ttgggcgaga tgcgggacga
     2401 cgcctggacg ccacccatct cgcactgcac gtcttcggcg atgaagcgct tgaggaagtc
     2461 gacgacctcc ggtcgcgccg cgttcttccc gacgacggtc agctcgccgg cgtacagcgt
     2521 gccgtcctgg tcgatcggcg ggaacgcgaa ccagtcgtag tcgacaccgg cctgcgctgt
     2581 ctcggggaac gacgcgttga tgaagctcgc ctggcggtgc agccagcact tcggcggcgt
     2641 gtcgaacatg ggcaacggcg cgtcgccgaa cgcgatgtcg ggcgtcgccg ccgcgccacc
     2701 gagcacgtag ccgtcggtga acatgacctt gccgaaggtc tcacccgcgg tgcgcaccgc
     2761 gtcgtcgttg aacgggatct cgtgcgcgac ccactggtcg tagacctcag gtccggcagt
     2821 ccggagcatg atgtcttcca tccagtcggt ggccggccaa cccgtggagc cttcgctgcc
     2881 gaatccgacg caccacggcg tgctgccgtc ggcgacgatc tggtcgctca acgcgatcag
     2941 gtcgtcgaac gtcttcggca cctggtatcc ggcggcatcg aagtcgtcct tcgggtacca
     3001 gatcatgctc ttcaggttga tgttggtcgg gatgccgtag tgcttgccgt tgtactcgcc
     3061 gagcgcgacg aacgactcgc cgacgaggtc gttgagctcc tggatgtcga agccgaggtc
     3121 ctcgagcgag acgatcgaac ccgagtcgac gagcgccggg atcgagccgg gctgcggcgc
     3181 ggcggcgacg tcgagcgtgc caccctcggc gcggatctgg aactgctctt cgaagtcgcc
     3241 gaccgactcg acctcgacgt tgtagtcggt cttcgagttg atcaggtcgt cgaagatcgt
     3301 ctggtacgcg tcgacctctt cgggttcacc ggcactcatc aaattcacag tgccggtgtt
     3361 ctcacccatt tcgaaaccgc cgccatcacc tccgccactc gtcgagtcat cgtcgtcatc
     3421 gtcgccgccg caccccacag caacgaacgc gacgaccatg agtggaacca acatcgtgcg
     3481 taaccgcatc agtgctttcc cccttgcatg gactgtcgtt gtgtcgcggt ggcacctcac
     3541 atcaggagcc gccgtagatg ccgagcccat ccttggggtc gaagaaatgc aggcgttgcg
     3601 cgtcgacgac cagctcgatc gaatctccct tgcggaccgc agtccgccgg tcgactcggg
     3661 cgaccatcgt cgaccgacct ccctgtgcct gggcttgcac ttgctcgagc gcttcggtgc
     3721 ccacctcgac ggcgagctcg acggcgtcct cggccatggc cgggggcgcg tcgatggtga
     3781 agtgcacgac aacatcggag ccgagcgact cgcgcagctc gacggtggac cggatacggc
     3841 ggtcggcggg cgcatcggac acgagggctg cgtcctccat gtcctcgggc cggatgccga
     3901 gcacgatgac ctggtcctcg tatccctcga gatcggcgtg cgccgccatc gtctcgtcgg
     3961 gaagcgagac gcagaagccg ccgaacacga cggcgaagcg gccgccggtg cgccgcagcg
     4021 tcgcgtcgaa caagttcatc gcgggcgagc cgatgaatcc gccgacgaag atgttgaccg
     4081 ggtgctcgta gagcgtctgt ggattgtcga cctgctgcaa cctgccgcgc cgcagcaccg
     4141 cgacacgatc gcccatcgtc atcgcctcga cttggtcgtg cgtgacgtag atcgtcgtga
     4201 cacccagctc gtgttggagc cgcgcgatct cggcgcgcat ctgtactcgc agtttcgcgt
     4261 cgaggttcga cagcggctcg tccatcaaga a
//
LOCUS       DASUGA010000028         2594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2075320, whole genome shotgun sequence.
ACCESSION   DASUGA010000028 DASUGA010000000
VERSION     DASUGA010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2075320"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2100
                     /locus_tag="VFX21_00560"
     CDS             <1..2100
                     /locus_tag="VFX21_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEX5094464.1"
                     /translation="LLVDDEPFDVRYGTLMHHERVLDLRGGVLRRTVEWASPAGQAVH
                     VRSTRLVSFVHRSVAAVRYEVEAVDTAARVVVQSSLVANEPGPERADDPRAAAALRAP
                     LIGEYHTHHDLEASLGHRTRASGLRMAAALDHVVHGPPRTITDSESEPDLARITVSTE
                     LAPGQSLTVVKLLTYGWSAERSMPALRDQVDAALASARRTGWDGLVVDQRTYLDEVWR
                     RADVEIDGDPELQQAARFAVFQLVQAAARAERRAIPAKGLTGRGYDGHTFWDTETYTL
                     PVLTYTAPVAARDALAWRHTTLPLARARARELRLGGAAFPWRTIRGEECSGYWPAGTA
                     GFHINADVADAVRRYVAATGDDEFEEGPGFELLVETARLWASLGHHDAEGRFRVDGVT
                     GPDEYTALVDNNTYTNLMAARNLRAAADAATRYPGRATEIGVDDTEIDAWRAAADAIV
                     IPFDPELGVTPQSAGFTRYRRWDFDATPPDAYPLLMHYPYYLLYSSQVVKQADLVLAL
                     YLCGDVFDLDQKRRDFAYYEPITVRDSSLSASIQAILAAEVGHADLAYQYWRETALVD
                     LRDLSGNTADGVHLAALAGTWLAAVAGFGGLRDHGETLAFSPRLHPPLSRLCFRLLYR
                     GRRLRVEIVPGRATYELHEGDPVEVLHHGVRIELRQGSPVTVAWETHPQGHASVAPPP
                     GRDPLCDRSQGRGGRVR"
     gene            2103..>2594
                     /locus_tag="VFX21_00565"
     CDS             2103..>2594
                     /locus_tag="VFX21_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5094465.1"
                     /translation="MRPHYRHVLVPLDGSELAAAAMRTADVLAARFGAHLHTVSVVQS
                     TAEVATIRTHAAELGVDDERVHVIVGDDPAAGIDARRGELDPCLVCLSTHGRGRLSGA
                     LVGSVARAVLTAASAPIVCVGPFADRPRPFRESYLPPPLSVPRLVACVDGGPEDEGIL
                     PIAV"
BASE COUNT          387 a          883 c          882 g          442 t
ORIGIN      
        1 ctgctcgtgg acgacgagcc gttcgacgtg cgttacggca cgttgatgca tcatgagcga
       61 gtgctcgacc tgcgtggtgg tgtgctacgg cgcaccgtcg agtgggcatc gccggccggt
      121 caggcggttc atgtgcgttc gacgcggctg gtgtccttcg tgcatcggtc tgttgccgcc
      181 gttcgttacg aagtggaggc ggtcgacacc gcggcccggg ttgtcgtgca gtcgtcgctc
      241 gttgccaacg aacctggtcc ggaacgggcg gacgatcctc gcgccgcggc cgcgctgcgg
      301 gcaccgctga tcggggagta ccacacgcat catgacttgg aggcttcgct cggtcatcgc
      361 acgcgcgcga gcggccttcg tatggcggcc gctctcgatc acgtggtcca cggccccccg
      421 cgaacgatca cggactcgga gagtgagccc gacttggcgc gcatcaccgt gagcacggag
      481 ctcgcgcctg gtcaatctct gacggtcgtc aagctgctca cctacggctg gtccgcggag
      541 cggtccatgc cagcattgcg cgaccaggtc gacgcggcgc tcgcctcggc ccggcgtaca
      601 ggctgggacg gtctcgtcgt cgaccagcgc acgtatctcg atgaggtatg gcgccgtgcc
      661 gacgtagaga tcgacggcga tcccgagttg caacaggccg cgcgattcgc cgtgttccaa
      721 ctggtgcagg cggcggcgcg ggccgagcgc cgggcgatcc ctgcgaaagg gttgacgggt
      781 cgtggctacg acggccatac gttttgggac accgagacct acacgcttcc cgtgttgacc
      841 tacaccgcac ccgtggcggc gcgtgatgcg ctcgcatgga gacacacgac gcttccgttg
      901 gcgcgcgctc gggcgcgcga gctgcgtctc ggtggtgccg cgttcccatg gcggacgatc
      961 cgaggcgagg agtgctccgg ctactggccc gcggggacgg ccgggtttca catcaatgcc
     1021 gatgtcgccg acgcggtccg tcgttacgtt gccgcgaccg gcgatgacga gttcgaggaa
     1081 ggaccaggct tcgagctgtt ggtcgagacc gcacggctct gggcgtcgct cgggcatcac
     1141 gacgccgagg gccgcttccg ggtcgacggc gtgacgggac ccgacgagta cacggcgctc
     1201 gtcgacaaca acacgtacac gaacctcatg gccgcgcgca atctgcgcgc cgctgcggac
     1261 gcggcgacgc gctatcccgg gcgcgcaacc gagatcggcg tcgacgacac tgaaatcgac
     1321 gcgtggcgcg ccgcggccga tgcgattgtc ataccgttcg accccgaact cggtgtcacg
     1381 ccgcagtcgg cgggattcac tcggtaccgc cgatgggact tcgacgcgac gccgcccgac
     1441 gcctacccac ttctgatgca ctatccctac tacttgttgt actcgagtca ggtcgtgaag
     1501 caggccgacc tcgtcctcgc gctgtatctc tgcggcgacg tgttcgacct ggaccagaag
     1561 cgacgggact tcgcgtacta cgagccgatc acggtgcgcg actcgtcgtt gtcggcttcg
     1621 atccaggcaa tcctcgcggc cgaagtggga cacgccgacc tcgcgtatca gtactggcgg
     1681 gagaccgccc tcgtcgacct gcgcgacttg tccggcaaca ccgcagacgg cgtgcatctc
     1741 gcggcactcg ccggcacgtg gctcgctgcc gtcgcgggct tcggcggcct acgcgaccat
     1801 ggcgaaacgc ttgcattctc accacgcctt catccaccgc tgtcgcggtt gtgtttccgg
     1861 ctgctgtatc ggggtcgtcg cctgcgggtc gagatcgtgc cgggccgcgc gacatatgag
     1921 ctgcacgaag gtgacccagt cgaggtcctc caccacgggg tacgaatcga gctgcgccaa
     1981 ggatctcccg tgacagtcgc gtgggagaca catccgcaag gccacgcgag cgtcgcgcca
     2041 ccgcccggtc gtgacccgct gtgcgatcgg agccagggcc gaggcgggag agtccgctga
     2101 tgatgcgacc tcactatcga cacgtcctcg tgcccctcga cggctcggaa ctcgcggccg
     2161 cggcgatgcg caccgccgac gtactggcgg cacgcttcgg cgctcacctg cacacagtga
     2221 gcgtcgtgca aagcacggcc gaagttgcca cgatccgaac gcacgccgcg gagctcggcg
     2281 tcgacgacga gcgcgtgcac gtgatcgtcg gcgacgaccc cgcggcgggc atcgacgcac
     2341 ggcgaggaga gctcgatccg tgtttggtgt gtctgtcgac gcacggccga ggccggctga
     2401 gcggggcgct cgtcggctcc gtcgcgcgtg cagtactcac cgccgccagc gctccgatcg
     2461 tatgcgtggg tccgttcgcc gatcgcccgc gtcccttccg cgagtcgtac cttccgccgc
     2521 cgctctccgt gccgaggctc gtcgcgtgtg tcgacggcgg acccgaggac gaaggcatct
     2581 tgcccattgc cgtc
//
LOCUS       DASUGA010000029         4079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_270226, whole genome shotgun sequence.
ACCESSION   DASUGA010000029 DASUGA010000000
VERSION     DASUGA010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270226"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2079)
                     /gene="gyrA"
                     /locus_tag="VFX21_00570"
     CDS             complement(<1..2079)
                     /gene="gyrA"
                     /locus_tag="VFX21_00570"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX5094466.1"
                     /translation="MPDETQSPLSNIEPIEIQEEMERSFLDYAMSVITARALPDARDG
                     LKPVQRRILYGMYVEGMRPDRAHRKSANAVGAVMAAYHPHGDSAIYDAMARMAQDFSL
                     RYPLVDGHGNFGSPDPADRPAASRYTEARLAPLAMDLLGEIDENTVDFNDTYDGQHQE
                     PAVLPARFPNLLVNGGGGIAVGMATNIPPHNLREVIDATLHLIDNPEATTEDLMQFVL
                     GPDFPTGATILGRGGIIEAYRTGRGSIRMRAVADIDESARGGQRIVVTEVPYQTSVEV
                     IGTKIAELVNERRIEGIRDVRNESAGDTTRLVIELKRDANAQVVLNQLYKHTPMQSSF
                     AANMLALVDGVPRLLTLQHALQVYVAHQIEVVTRRSEYRLGKARDRAHILEGLLRALD
                     MIDAIITLIRGSESADAARTALQAEPFSFSDVQATHILDMALRRLAALERQKLADEYA
                     ELQATIAELEAILADEIKLRAVIKEELTVIRDKYGDDRRSTLSHDPGELLDLDLIEDE
                     ELVVQLSHRGYVKTVQVDQFRTQGRGGKGVRGGNLRDEDYVEHLLTSTAHSYLLFFSN
                     RGRVYRLRAHEIPMKDRTARGTALVNLIALQPDEHIEAIIDTRTYEDGAYLFFATRRG
                     MVKKTRMSEYDSSLRTGLIAINLNDGDELVRVIQTSGSDDVFMVSQQGMTIRFSEDDV
                     RPMGRATAGVR"
     gene            complement(2108..4015)
                     /gene="gyrB"
                     /locus_tag="VFX21_00575"
     CDS             complement(2108..4015)
                     /gene="gyrB"
                     /locus_tag="VFX21_00575"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX5094467.1"
                     /translation="MAYAAKDISILKGLQPVRERPGMYIGSTGPSGLHHLVYEVVDNS
                     VDEALAGYANRIDVTLLRDGGCRVVDDGRGIPVDPHPEYPDKSAAEIVLTMLHAGGKF
                     GGEGYKISGGLHGVGVSVVNALSRRLELVIDRDGGRYKQVFEDGGEPTGPLQRLGDSD
                     ITGTSVTFWPDPTIMEEVEFRAQTLVERLREMAFLNKGLEIVFRDERPDEPVEQVFKF
                     DGGIVDFVAHLNASKEPLFATVISLGDTYDEGEVEIAMQWNTGYYEGLHSFANNIATT
                     EGGMHEEGFKKSLTNVVNRYAKAKGQIKDKDENFLGEDIREGLTAIISVKLRSPQFEG
                     QTKTKLGNTEIRSFVERVTNDKLAEWLEEHPAEAKAIVSKSQQSARARVAARQARDLT
                     RRKSLLESASMPGKLADCSSRNPEEAELFIVEGDSAGGSAKKARNPAFQAILPIRGKI
                     LNVERARMDKTLRNEEIQALISAAGTGIGEEFDLSKLRYHKIIIMSDADVDGSHIRTL
                     LLTFFYRQLKEIVNAGHVYIAQPPLYGAKIGKDDRTYLKDDAALRAFEAEHEGRKIEI
                     SRFKGLGEMDWQELGVTTMDPATRTLLQVNALDSPTIADEVITDLMGDDVDKRRTFIQ
                     QNAKDVRFLDI"
BASE COUNT          582 a         1291 c         1411 g          795 t
ORIGIN      
        1 ccggacgccg gccgtcgccc ggcccatcgg gcggacgtcg tcttcggaga agcggatcgt
       61 catgccctgc tgcgacacca tgaagacgtc gtcgctcccg ctcgtctgga tgacgcgcac
      121 gagctcgtcg ccgtcgttca ggttgatcgc gatgaggccg gtgcgcagcg acgagtcgta
      181 ctccgacatg cgcgtcttct tcaccatgcc ccgccgcgtc gcgaagaaca gatatgcgcc
      241 gtcttcgtac gtgcgcgtgt cgatgatcgc ttcgatgtgc tcgtcgggtt gcagcgcgat
      301 gagattgacg agcgccgtac cgcgcgcggt gcggtccttc atcgggatct cgtgcgcgcg
      361 cagtcggtag acgcggccgc gattcgagaa gaacagcaag tacgagtgcg cggtgctcgt
      421 gagcaagtgc tcgacgtagt cttcgtcgcg gaggttgccg ccgcgcacac ccttgccgcc
      481 gcggccctgc gtgcggaact ggtcgacctg cacggtcttg acgtagccgc ggtgtgacag
      541 ctgcacgacg agctcttcgt cctcgatgag gtcgaggtcg agcagttcgc cggggtcgtg
      601 gctgagggtg ctccggcggt cgtcgccgta cttgtcgcgg atgaccgtca gctcttcctt
      661 gatgacggcc cgcagcttga tctcgtcggc caggatcgcc tccagctcgg cgatcgtggc
      721 ctggagctcg gcgtactcgt cggccaactt ctggcgctcg agcgcggcca ggcggcgcag
      781 cgccatgtcg aggatgtgcg tggcctgcac gtcgctgaac gagaacggct cggcctgcag
      841 cgcggtgcgc gccgcgtcgg ccgactccga cccccggatc aacgtgatga tcgcgtcgat
      901 catgtcgagg gcgcgcagca gtccttcgag gatgtgcgcc cggtcgcgcg ccttgcccaa
      961 ccggtactcg ctgcgacggg tgacgacttc gatctggtgc gcgacataca cctgcaacgc
     1021 gtgctgcagt gtcaacaacc gcggcacgcc gtcgacgagc gcgagcatgt tcgcggcgaa
     1081 cgacgactgc atcggcgtgt gcttgtagag ctggttgagc acgacctgcg cgttggcgtc
     1141 acgcttgagc tcgatcacca gccgcgtcgt gtcaccggcg gattcgttgc ggacgtcgcg
     1201 gatgccttcg atgcgccgct cgttgacgag ctcggcgatc ttcgtgccga tcacctcgac
     1261 ggaggtctgg tacgggacct cggtgaccac gatgcgctgg ccgccgcgcg cgctctcgtc
     1321 gatgtcggcg acggcccgca tgcggatcga gccgcggccc gtacggtagg cctcgatgat
     1381 cccgccgcgg ccgaggatcg tggcgcccgt cgggaagtct ggaccgagga cgaactgcat
     1441 caggtcttcg gtggtggcct cggggttgtc gatcaggtgc agcgtcgcgt cgatgacttc
     1501 ccgcaggttg tggggcggga tgttggtggc catgcccacc gcgatgccgc caccgccgtt
     1561 gaccaacagg ttcgggaagc gcgccggcag gaccgccggc tcttggtgct gaccgtcgta
     1621 ggtgtcgttg aagtcgaccg tgttctcgtc gatctcaccg agcagatcca tcgcgagcgg
     1681 cgccagccgc gcttcggtgt aacgcgaggc agcggggcgg tcggccgggt cgggtgaacc
     1741 gaagttcccg tgaccgtcga cgagcgggta ccgcagcgag aagtcttggg ccatgcgcgc
     1801 catcgcgtcg tagatcgcgc tgtcgccgtg cgggtggtac gccgccatca ccgcaccgac
     1861 ggcgttggcc gacttgcggt gggcacgatc gggccgcatc ccctcgacgt acatcccgta
     1921 gaggatccgg cgttgcaccg gcttgaggcc gtcgcgcgcg tcgggcaacg cgcgcgcggt
     1981 gatgaccgac atcgcgtagt ccaggaagga gcgttccatc tcctcttgga tctcgatcgg
     2041 ctcgatgttg ctcagcgggc tctgggtctc gtcgggcacg cgaaaaggcc tttgttcttc
     2101 gtgcgttcta gatgtcgagg aagcgaacgt ctttggcgtt ctgctggatg aacgtgcgcc
     2161 gcttgtcgac gtcgtcgccc atgaggtcgg tgatgacttc gtcggcgatg gtggggctgt
     2221 cgagcgcgtt gacctggagc agtgtgcgcg tcgcgggatc catggtggtg acgccgagct
     2281 cctgccagtc catctcaccg agacccttga accggctgat ctcgatcttg cgcccttcgt
     2341 gttccgcctc gaacgcgcgc agcgcggcgt cgtccttcag gtacgtgcgg tcatccttgc
     2401 cgatcttggc gccgtacaac ggcggctgcg ctatgtacac gtggccggcg ttcacgatct
     2461 ccttgagctg ccgatagaag aacgtcaaca gcaacgtgcg gatgtgtgag ccgtcgacgt
     2521 cggcgtcgct catgatgatg atcttgtggt agcggagctt cgagaggtcg aactcctcgc
     2581 cgatgcccgt gccggccgcg gagatcaacg cctggatctc ttcgttgcgc agcgtcttgt
     2641 ccatacgggc gcgctcgacg ttgaggatct tgccgcggat gggcaggatc gcctggaacg
     2701 ccgggttgcg ggccttcttg gcgctcccgc ccgcgctgtc gccctcgacg atgaacagct
     2761 cggcctcttc gggattgcgc gacgagcagt cggcgagctt gcccggcatc gacgccgatt
     2821 cgagcagcga cttgcggcgg gtgaggtcgc gcgcctgccg ggccgccacc cgcgcgcggg
     2881 ccgactgttg cgacttgctg acgatcgcct tggcctcggc ggggtgctcc tcgagccact
     2941 ccgcgagctt gtcgttggtg acgcgctcga cgaacgaacg gatctcggtg ttgccgagct
     3001 tcgtcttcgt ctgtccctcg aactgcgggc tgcgcagctt caccgagatg atcgcggtga
     3061 gaccctcgcg aatgtcttcg ccgaggaagt tctcgtcctt gtccttgatc tgccccttcg
     3121 ccttcgcgta gcggttcacg acgttcgtga gcgacttctt gaagccctct tcgtgcatgc
     3181 cgccttcggt ggtggcgatg ttgttcgcaa aggagtggag gccttcgtag taaccggtgt
     3241 tccactgcat cgcgatctcg acttcgccct cgtcgtaggt gtcgccgagt gagatcaccg
     3301 ttgcgaacaa gggctccttc gacgcgttga ggtgggcgac gaagtcgacg atgccgccgt
     3361 cgaacttgaa gacctgctcg accggttcgt cgggccgttc gtcgcggaag acgatctcga
     3421 ggcccttgtt gaggaatgcc atctcgcgga ggcgctcgac gagcgtctgg gcgcggaatt
     3481 cgacttcctc catgatcgtg gggtcgggcc agaacgtgac cgacgtgccg gtgatgtcgg
     3541 agtcgccgag gcgctgcagc gggccggtgg gctcgccgcc gtcttcgaag acctgcttgt
     3601 agcgaccgcc gtcgcggtcg atgacgagct cgagccggcg cgacagcgcg ttcaccacgg
     3661 acacgccgac accgtgcaga ccgccggaga tcttgtagcc ctcgccgccg aacttgccgc
     3721 cggcgtggag catcgtcagc acgatctcgg ccgcggactt gtcggggtac tcggggtggg
     3781 ggtcgacggg aatgccgcgg ccgtcgtcga cgacgcggca cccgccgtcg cgcaacaggg
     3841 tgacgtcgat gcggttcgcg tatcccgcaa gggcttcgtc gacggagttg tcgacgacct
     3901 cgtagacgag gtgatgcagg cccgacggac ccgtcgagcc gatgtacata ccgggccgct
     3961 cgcgcaccgg ctgcagcccc ttgagaatcg agatgtcctt ggccgcgtac gccacgcgtt
     4021 catgtccttg ggagaggcgg ttttcgggcg cgaaatccct ggtcagaggg ggtttcgcg
//
LOCUS       DASUGA010000030         2816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1240732, whole genome shotgun sequence.
ACCESSION   DASUGA010000030 DASUGA010000000
VERSION     DASUGA010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240732"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1333
                     /locus_tag="VFX21_00580"
     CDS             185..1333
                     /locus_tag="VFX21_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HEX5094468.1"
                     /translation="MVYCRSCLIPETKPDIFFDDEGVCSACRHFAGRGEVDWDARRRE
                     LDEVLERYRSKDGTTYDCIIGVSGGKDSTTQVIRMLEFGMNPLCVTATTDELSDIGRR
                     NIENLKNLGVDFVEYTTNPIARRKMNKLALVQVGDISWPEHVSIFTLPVRLAVQMGIK
                     LIVWGENPQNEYGGPAAAATGNTLTRRWLEEFGGLLGLRVSDLVGQDGIETKHLIPFT
                     YPSDEDLQRVGVTGIFLGYYLPWDGLQNAIFAQSYGLETWPGMIEGSLCNYENLDNHQ
                     TGIHDYFKFIKYGFGRATDIASMHVRRGRLRRDDALKLVRMHDGKFPWTYLGKPIEET
                     LAGIGMTFDEFIVVCDRFTNKRLFVTNRRGELVRGADQHLTKINDDNV"
     gene            1338..1955
                     /gene="hisH"
                     /locus_tag="VFX21_00585"
     CDS             1338..1955
                     /gene="hisH"
                     /locus_tag="VFX21_00585"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX5094469.1"
                     /translation="MERVAIVDYHLCNVDSVKRAFETLGAKAFITDVPSELAEADRIV
                     LPGVGAFPDAMRNLHDRGLDDALAVNVLEEGAPFLGVCLGMQLLATIGHEVTESKGLG
                     WFDASISRLVATEEDPRIPHVGWNEVSPVRDSPLFDGIPPQADFYFVHSFHMTCAHEA
                     DVLATTQYCGGFTSVVQREHVFGVQFHPEKSQQWGLRLLKNFLSV"
     gene            1957..2700
                     /locus_tag="VFX21_00590"
     CDS             1957..2700
                     /locus_tag="VFX21_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006278600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase cyclase
                     subunit"
                     /protein_id="HEX5094470.1"
                     /translation="MLKIRVIPTLLHKSWGLVKGKQFDSLRAVGSPMQAVKVYNLRNV
                     DELVFLDVSATGEGRGPDFDLVDELADECFMPLAVGGGIRTLDDVRHLLAVGADKVVL
                     GTVALEEPDLVREASDRFGAQCVVVSIDTKRDGSVWTNSGTVPTGRDALDVAQQMEAL
                     RAGEVLLQSVADDGMMDGYDVDTIGRVSAAVSVPVIASGGAGNFDHMVDAVRAGGTAV
                     AAASIFHFTEQTPMEAKQALSAAGFPTRV"
BASE COUNT          511 a          875 c          926 g          504 t
ORIGIN      
        1 cgagtgctgt cccggccggt ccgcccgccg atataaaaac gtgactagcc aggcgcagac
       61 cgcaggtgcg ggcaacgagc tgacgggtag cggtgatcga gcaccggagg gcggagccgc
      121 attgggcaac gttcaagccg cgaccgatca gctcatccag agtcgtcggg ccgagcagcg
      181 gccgatcgtc tattgccgga gctgtctcat tcccgagacc aagcccgaca tcttctttga
      241 cgacgagggt gtgtgcagcg catgccgtca cttcgccggg cgcggcgagg tcgactggga
      301 tgcgcgccgc cgagagctcg acgaggtgct cgagcgttac cgctcgaagg acggcacgac
      361 atacgactgc atcatcggtg tgagcggcgg caaggactcg accacgcagg tcatccggat
      421 gctcgaattc ggcatgaacc cgttgtgcgt caccgcgacg accgacgagc tctccgacat
      481 cggccgccgc aacatcgaga acctgaagaa cctcggggtc gacttcgtgg aatacacgac
      541 gaacccgatt gcgcggcgca agatgaacaa gctcgcgctc gtgcaggtcg gcgacatttc
      601 gtggccggag cacgtgtcga tcttcacgct gccggtgcgg ttggccgtgc agatggggat
      661 caagctcatc gtgtggggcg agaacccgca gaacgaatac ggcggtccgg ctgcggccgc
      721 gaccggcaac acgttgacgc gtcgctggct cgaagagttc ggcggtctgc tcggactgcg
      781 tgtttccgat ctcgtcgggc aggacggcat cgagacgaag catctgattc cgttcacgta
      841 tccgtcggac gaagatcttc agcgggtcgg cgtcacgggc atcttcctcg gctactactt
      901 gccgtgggac ggcctccaga acgcgatctt cgcccagtcg tacgggctcg agacgtggcc
      961 gggcatgatc gagggctcgt tgtgcaacta cgagaacctc gacaaccatc agaccggcat
     1021 tcacgactac ttcaagttca tcaagtacgg attcggccgc gcgacggaca tcgcgtcgat
     1081 gcacgttcgg cgcggccgcc tgcggcgcga cgacgcgctg aagcttgtgc gcatgcacga
     1141 cggcaagttc ccgtggacgt acctgggcaa gccgatcgag gaaacgcttg ccggcattgg
     1201 tatgacgttc gacgagttca tcgtggtgtg cgaccgtttc accaacaagc ggttgttcgt
     1261 gacgaatcgg cgcggcgaat tggtgcgggg cgccgatcag cacctcacca agatcaacga
     1321 cgacaacgtg tagtcccgtg gaacgcgttg ccattgtcga ctaccacttg tgcaacgtcg
     1381 actcggtcaa acgcgccttc gagacgctcg gcgccaaggc gttcatcacc gacgtgccgt
     1441 cggagctggc cgaagccgat cgcatcgtgc tgcccggtgt cggcgcgttt ccggatgcga
     1501 tgaggaacct gcacgaccgt gggctcgacg acgcgctcgc ggtcaacgtg ttggaggaag
     1561 gcgcaccgtt cctcggcgtg tgcctcggta tgcagctgct ggcgacgatc ggccacgagg
     1621 tgacggaaag caaaggcctc ggctggttcg acgcatcgat ctcacgcctc gtcgcgaccg
     1681 aggaagaccc tcgtatcccg cacgtcggct ggaacgaagt gagtccggtg cgtgactcgc
     1741 cgttgttcga tggcatcccg ccccaagccg acttctactt cgtgcactcg ttccacatga
     1801 cgtgcgcgca cgaagccgac gtgctggcga cgacgcagta ctgcggcggc ttcacgtctg
     1861 tggtgcagcg cgagcacgtg ttcggcgtgc agttccatcc cgaaaagagc cagcaatggg
     1921 gattgcggct cctgaagaac ttcttgtcgg tgtgacatgt tgaagatccg cgtcatcccg
     1981 acgttgctgc acaagtcatg gggcttggtg aaaggcaagc agttcgactc gctgcgcgcg
     2041 gtcggcagcc cgatgcaagc cgtcaaggtg tacaaccttc gcaacgtcga cgaactcgtc
     2101 ttcctcgacg tctccgcgac cggcgaaggg cgcgggcccg acttcgatct cgtcgacgag
     2161 cttgccgacg aatgtttcat gccgctcgcg gtcgggggcg ggattcgtac cctcgacgac
     2221 gtgcggcacc tgctggcggt cggagccgac aaagttgtgc tcgggacggt cgctctggaa
     2281 gaaccggatc tcgtgcggga agcgtcggac cggttcggcg cgcagtgtgt ggttgtctcg
     2341 atcgacacga agcgcgacgg ctcggtgtgg accaactccg gcacggttcc gaccggacgc
     2401 gacgcattgg atgtcgcgca acagatggaa gcactgcggg ccggtgaagt gctgctccaa
     2461 tcggtcgccg acgacggcat gatggacggc tacgacgtcg acaccatcgg gcgcgtgagt
     2521 gccgcagttt cggttccggt gatcgcgtcg ggcggagccg ggaacttcga tcacatggtc
     2581 gacgccgtac gcgccggcgg caccgccgtc gccgcggcaa gcatcttcca cttcaccgag
     2641 cagactccga tggaagccaa gcaggcgctc agcgccgccg gcttcccgac gcgggtctag
     2701 cctcgcaact tcgcctcgag tccggctctc accgccggcc attgttcgtc ggtgatcgag
     2761 aacatggctg agttgcggag cttgccttcc tcaccggcga cgtgcgactc ctgcca
//
LOCUS       DASUGA010000031         2186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2075099, whole genome shotgun sequence.
ACCESSION   DASUGA010000031 DASUGA010000000
VERSION     DASUGA010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2075099"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VFX21_00595"
     CDS             <1..234
                     /locus_tag="VFX21_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094471.1"
                     /translation="LDDTLQQVAREHPDDVVDVIEMSDLVCPDWRTTNSCPDTKPGGG
                     FYRGDDGEHFDGRAAVEAGQWLVDRIKALRVTP"
     gene            complement(217..1359)
                     /locus_tag="VFX21_00600"
     CDS             complement(217..1359)
                     /locus_tag="VFX21_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HEX5094472.1"
                     /translation="MAERCAVIGVGQTHHKKVRDDVSIPGLLREAALRALEDAELTFA
                     DIDALVIGKAPDAFEGIVQPELFLADALGAAGKPMLRVHTAGSVGGSTFIVGSHLIES
                     GIHERVLALSWEKQSEGNAQWGLAGGRSRGIGAGGAFAPWIRAYIEETGAPEYIGWQV
                     AVKDRLNALKNPYAHLKIADISIEKVKESVMLWDPLRFLESCPSSDGACAVVLTNEAG
                     GKKASKPAAWVHATAVKSELGQFPGRNPVRPQAGVECAHDVYKQAGISVPRQEIDVAE
                     LYVPFSWYEPMWLEGHDIAGPGEGWKMVESGDTEITGSFPVNPSGGVLSSNPIGASGM
                     LRFAEAALQVRGLAGEHQVDGVKTALAQAYGGAAQYFAMGVLRSNP"
     gene            complement(1362..>2186)
                     /locus_tag="VFX21_00605"
     CDS             complement(1362..>2186)
                     /locus_tag="VFX21_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007458473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HEX5094473.1"
                     /translation="ESHVEMDGAWAMHEAWVRLQHGDIDTALVFGSGKSSPSNPYEMW
                     AQQLDPYYLAPLGVDPVSLAGLQARALLDAGKASERDFAEVAVRSRANAKNNPNAQVA
                     GDFSADDLLKQPYVREPLRKHDLPPISDGAVAVVMARGDKAKALCKRPAWIRGFDHRI
                     EVHQPGMRDLTTSPSTTLAARKANYDGGPLDVVELSATFSPQELILREALGLGADANV
                     NPSGGALAANPVMATGLVRIAEVAQRIVDGEANRGLAHATGGQVLQHNLVVLMEGE"
BASE COUNT          351 a          794 c          676 g          365 t
ORIGIN      
        1 ctcgacgaca ccctgcagca agtcgcgcgt gagcacccgg acgacgttgt cgacgtgatc
       61 gagatgagcg acctcgtgtg tcccgattgg cgcacaacga attcatgccc cgacacgaaa
      121 ccgggcggcg ggttctaccg cggcgacgac ggcgaacact tcgacggccg cgccgccgtc
      181 gaagccggcc aatggctggt cgatcggatc aaagccctac gggttactcc gtagcactcc
      241 cattgcgaag tactgcgcgg cgccgccgta ggcctgcgcg agcgccgtct tcacgccgtc
      301 gacttggtgc tcgcccgcga ggccgcgcac ctgcaacgcc gcctccgcga accgaagcat
      361 gccgctcgcg ccgatcgggt tcgacgacag cacaccgccg ctcgggttga ccgggaacga
      421 accggtgatc tccgtgtcgc cgctctcgac catcttccag ccttcgcccg ggcccgcgat
      481 gtcgtggccc tcgagccaca tcggttcgta ccagctgaac ggcacgtaga gctcggcaac
      541 gtcgatctcc tggcgcggca cgctgatgcc ggcctgcttg tagacgtcgt gcgcgcactc
      601 gacgcccgcc tgcggacgca ccggattgcg gcccgggaac tggccgagct cgctcttcac
      661 cgcggtggca tgcacccacg cggccggctt cgacgccttc tttccaccgg cttcgttcgt
      721 caacacgacc gcgcacgcgc catcggaact cgggcatgac tccaggaacc gcaacgggtc
      781 ccagagcatc accgactcct tcaccttctc gatgctgatg tcggcgatct tcaagtgcgc
      841 gtacgggttc ttcagcgcgt tcaggcggtc ctttaccgcg acttgccagc caatgtattc
      901 gggtgcgccg gtctcctcga tgtaggcacg gatccagggc gcgaatgcac cacccgcgcc
      961 gatgccgcgc gaccggccgc ccgcgagacc ccattgcgcg ttgccctcgc tctgtttctc
     1021 ccacgagagc gccagcacgc gctcgtgaat gcccgactcg atcaagtgcg agccgacgat
     1081 aaacgtcgag ccgccgaccg agcctgcagt gtggacgcgc aacatcggct tgcccgcggc
     1141 gccgagcgcg tcggcgagga acagctcggg ctgcacgatg ccctcgaacg cgtcgggcgc
     1201 cttgccgatg acgagcgcgt cgatgtcggc gaacgtcagc tccgcgtctt ccaatgcgcg
     1261 caacgccgct tcccgcaaca accccgggat cgagacgtcg tcgcggacct tcttgtgatg
     1321 cgtctggccg acgccgatga cggcacaacg ctcagccata gttattcacc ctccatgagc
     1381 acgacgaggt tgtgctgcag cacttgtccg ccggtcgcat gcgcaagacc gcggttcgct
     1441 tcaccgtcca cgatgcgttg cgcgacttcg gcgatccgga ccaagccggt cgccatcacc
     1501 gggttcgcgg cgagcgcacc acccgacgga ttcacgttgg cgtcggcgcc gaggccgagc
     1561 gcttcccgca agatcaactc ctggggcgag aacgtcgccg acagttccac cacgtcgagc
     1621 ggcccgccgt cgtagttcgc cttgcgtgcc gcgagcgtgg tcgacggcga cgtcgtcagg
     1681 tcgcgcatcc ccggctggtg cacttcgatg cggtgatcga aaccgcgaat ccaggccggg
     1741 cgcttgcaca acgcctttgc cttgtcgccg cgcgccatca ccaccgccac ggcaccgtcg
     1801 gagatcggcg gcaggtcgtg cttgcgcaac ggctcgcgca cgtacggctg cttcaacagg
     1861 tcgtcggccg agaagtcgcc cgcaacttgc gcgttcgggt tgttcttggc gttggcccgg
     1921 ctccgcaccg ccacctcggc gaagtcgcgc tcgctcgcct tgcccgcgtc gagcaacgcg
     1981 cgcgcttgta gtccggcgag cgagaccgga tccacaccca atggcgcgag gtagtagggg
     2041 tcgagctgct gcgcccacat ctcgtagggg ttcgacggcg acgacttccc agagccgaaa
     2101 accagcgcag tgtcgatgtc gccgtgctgc aagcgcaccc acgcttcgtg cattgcccac
     2161 gcaccgtcca tctcgacgtg cgactc
//
LOCUS       DASUGA010000032         2987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2075034, whole genome shotgun sequence.
ACCESSION   DASUGA010000032 DASUGA010000000
VERSION     DASUGA010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2075034"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VFX21_00610"
     CDS             complement(<1..495)
                     /locus_tag="VFX21_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5094474.1"
                     /translation="MAFDMIRIEHLSKHFGAVRAVDDVSLNVALREVVVILGPSGSGK
                     STVLRCINGLEVPTGGAIYVDNILVEPGKKSINAVREEVGMVFQQFNLFPHLTALENI
                     TMAQRVVRGRSKNEAKVIAREQLERVGIPEKADFRPRELSGGQQQRVAIARSLAMNPM
                     VMLFD"
     gene            complement(506..1324)
                     /locus_tag="VFX21_00615"
     CDS             complement(506..1324)
                     /locus_tag="VFX21_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX5094475.1"
                     /translation="MPEALDAVNPELSAPVIDIRRSRSWRDFPYWLVAIIGVIAWMAY
                     LIATQDRYNIAYDRIIRGLRLTVETTVYAFILAVVLGLIAGLGRIARNVVARNVAIFY
                     IEFIRGVPILVLIFTVAFVVVPEGTEFFDLENSTVSLQWRAIIALALIYGAYLGEVFR
                     AGIESVPTGQTEAGRSLGMSHRQTMRRIVLPQAVRNMTPALGNDAIAMLKDTSLISVL
                     AVRDLTQHARLYAGTSFDFRATYLVLVACYLTLTLTLSLLLAWYRRRLGLHSGI"
     gene            complement(1341..2147)
                     /locus_tag="VFX21_00620"
     CDS             complement(1341..2147)
                     /locus_tag="VFX21_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX5094476.1"
                     /translation="MASCGGDDDDDSASTDTTAAAASGDLPDLGGKSVTVAVENAYLP
                     FNYINPDTGEGAGWDYDAWNAICEKLNCKAEFTEAAWEGMIQAVGDGQYDAGADGITI
                     NDERAQVVDFSDGYIQVNQRLLVRSDEDRFENAEDVTADDSIKIGTQTGTTNYDKAVE
                     LWGEGRIQAFEQFPFAVEALINGDVDVVVMDETAGQGYVGANSSDLKLVGEPLVSDEL
                     GFIFPKGSDLVEPVNAALGALQEDGTMDALAQAYFSDDFTPTREKVEAAG"
     gene            complement(2229..>2987)
                     /locus_tag="VFX21_00625"
     CDS             complement(2229..>2987)
                     /locus_tag="VFX21_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5094477.1"
                     /translation="FDDPLVIAGQGTVALEMLAAAPELDTIVVPVGGGGLISGIATAA
                     KATRAGITVVGVQAEGYAGMLFALGRAPAPPGGPTIAEGIAVKEPGVLTREIVAELVD
                     DLLVVSEQHIEAAIALAIEIEKTVVEGAGAAALAAVVEHRDRFAGRRVGVVLSGGNID
                     LRVLSSVMMRALARSGRIVRLAIEVPDRPGVLAAVANVIGTCRGNIVDVTHRRDLPGV
                     ALKVTMLELSVETRDQAHADEIVRALQENGFPVI"
BASE COUNT          475 a         1005 c         1002 g          505 t
ORIGIN      
        1 gtcgaacagc atcaccatcg ggttcatcgc gagtgaccgc gcgatggcga cccgctgctg
       61 ctgcccgccc gagagctctc gcggccggaa gtcggccttc tcggggatgc cgacccgctc
      121 gagctgctcg cgcgcgatga ccttggcttc gttcttgctg cgcccacgta cgacgcgctg
      181 ggccatggtg atgttctcga gcgcggtcag gtgcgggaac aggttgaact gctggaacac
      241 catcccgact tcttcgcgca ccgcgttgat cgatttcttg cccggctcga cgaggatgtt
      301 gtcgacgtag atcgcaccgc cggtcggcac ttcgaggccg ttgatgcagc gcagcaccgt
      361 gctcttgccc gagccgctcg gcccgaggat caccacgacc tcgcgcagcg cgacgttcag
      421 gctgacgtcg tcgaccgcgc gcacggcgcc gaaatgcttc gacaggtgct cgatgcggat
      481 catgtcgaag gccatctcgt ctccctcaga tgccggagtg gaggccgagg cgccggcggt
      541 accaggcgag gagcaacgac agcgtcaacg tgagggtgag atagcacgct acgagcacga
      601 gatacgtggc gcggaagtcg aagctcgtgc ccgcgtagag gcgggcgtgc tgggtgaggt
      661 cacgcaccgc gagcacggag atgagcgagg tgtccttgag catcgcgatc gcgtcgttgc
      721 cgagcgcggg tgtcatgttg cgcacggctt gcgggagcac gatccggcgc atcgtctggc
      781 ggtgcgacat gccgagtgag cggcctgctt cggtctggcc ggtcgggacc gattcgatgc
      841 cggcccggaa cacctcgccg aggtatgcgc cgtagatgag cgcgagggcg atgatggccc
      901 gccactgcag gctgacggtg ctgttctcga gatcgaagaa ctcggtgccc tcgggcacga
      961 cgacgaacgc gaccgtgaag atcagcacca ggatcggcac gccgcggatg aactcgatgt
     1021 agaagatcgc gacgttgcgg gcgacgacgt tgcgggcgat ccgtcccagg cccgcgatca
     1081 accccagtac gacggcaagg atgaacgcat agaccgtcgt ctcgacggtg aggcgcaagc
     1141 ccctgatgat gcggtcgtac gcgatgttgt agcggtcttg cgtcgcgatc aggtacgcca
     1201 tccacgcgat gacgccgatg atcgcgacca gccagtacgg gaagtcccgc cagctccgtg
     1261 accttcggat gtcgatcacc ggagcactga gctccggatt gacggcgtcc agcgcttccg
     1321 gcatctagtg ctagcgagcg ctagccggcg gcttcgacct tctcgcgcgt cggcgtgaag
     1381 tcgtccgaga agtacgcctg ggcgagggcg tccatcgtcc cgtcctcctg cagcgccccg
     1441 agcgccgcgt tcacgggctc gacgagatcg ctgcccttcg ggaagatgaa gccgagctcg
     1501 tcgctgacga gcggctcgcc cacgagcttc aggtcgctcg agttcgcgcc gacgtagccc
     1561 tggccggccg tctcgtccat cacgacgacg tcgacgtcac cgttgatgag cgcctcgacc
     1621 gcgaacggga actgctcgaa ggcctggatg cggccctcgc cccagagctc gaccgccttg
     1681 tcgtagttcg tggtgccggt ctgcgtgccg atcttgatgc tgtcgtccgc ggtgacatcc
     1741 tccgcgttct cgaaacggtc ctcgtcactg cgcacgagca ggcgctggtt gacctgaatg
     1801 tatccgtcgg agaagtcgac gacctgtgcg cgctcgtcgt tgatcgtgat gccgtccgcg
     1861 cccgcgtcgt attgcccgtc accgaccgcc tggatcatgc cttcccacgc ggcttcggtg
     1921 aactcggcct tgcagttcag cttctcgcag atcgcgttcc acgcgtcgta gtcccaaccc
     1981 gcgccttcgc cggtgtcggg gttgatgtag ttgaacggga ggtacgcgtt ctcgaccgcg
     2041 accgtgacgc tcttgccgcc gaggtcgggc aggtcgccgc tcgccgccgc ggcggtggtg
     2101 tcggtggacg cgctgtcgtc gtcatcgtcg cctccgcacg acgccatcgc gattgccaat
     2161 gcactgatga gaattgcagc ggtccactta cgcacgccga gcccctcccc tagaacgatc
     2221 gcgggagact agatgacagg gaagccattt tcctggaggg cgcggacgat ctcgtcggca
     2281 tgcgcctggt cccgggtctc gaccgacagc tccagcatcg tcaccttgag cgcgacgccc
     2341 gggagatcac ggcggtgggt gacgtcgacg atgttcccgc gacaggttcc gatcacgttg
     2401 gcaaccgccg ccaggacgcc cggccggtcg ggcacctcga tggccagccg cacgatgcgt
     2461 cccgagcggg cgagcgcgcg catcatcacc gacgacagca cgcgcaggtc gatgttgccg
     2521 cccgagagga cgacgccgac tcgccggccg gcgaagcggt cccgatgttc cacgacggcc
     2581 gcgagtgcgg ccgcgccggc gccctcgacg accgtcttct cgatctcgat cgcgagcgcg
     2641 atggccgctt cgatgtgctg ctcggacacg acgagaagat cgtcgacgag ctccgcgacg
     2701 atctcgcgcg tcaacacgcc cggctccttg acggcgatgc cttccgcgat cgtcggaccg
     2761 ccgggcggcg cgggcgcccg acccagcgcg aacaacatgc ccgcgtagcc ctcggcttgt
     2821 acgccgacga ccgtgatgcc ggcgcgcgtc gccttggccg cggtcgcgat accggagatc
     2881 aacccgccgc cgccgaccgg cacgacgatc gtgtcgagct cgggcgcagc cgcgagcatc
     2941 tcgagcgcga ccgtcccctg gcccgcgatg acgagtgggt cgtcgaa
//
LOCUS       DASUGA010000033         2347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2074616, whole genome shotgun sequence.
ACCESSION   DASUGA010000033 DASUGA010000000
VERSION     DASUGA010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2074616"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1420)
                     /locus_tag="VFX21_00630"
     CDS             complement(<1..1420)
                     /locus_tag="VFX21_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HEX5094478.1"
                     /translation="MARENYIGGKWQGAQAGATDKVLNPATGEVLDEVAASGPADVDA
                     AVAAAAGAFEAWSQTTPRQRFEALTKVADAIETDMATIRELEIKNVGKPLSIIEFEMD
                     LTVDNWRFFAAGARFLEGRAAGEYMEDHTSFVRRDPLGVVASIAPWNYPLNMATWKLG
                     PALAAGNTVVLKPSELTPLTALRLAEITADILPPGVLNVVTGDGEHTGAALVAHPDVA
                     MVSLTGDVATGKAIARAAADSLKRVHLELGGKAPVVVFDDADVEALVAVLTETGYYNS
                     GQDCTAPCRVLAGAGVFDTLVSDLTDSVGKIATGDPFDENTGMGPVISGEQLARVQGM
                     VDRARDGGAEVTTGGRVREGAGYFYEPSVVVNPDQGSEIVQREVFGPVISVQRFTDDD
                     QALAWANDVEYGLSASVWTRDVGRATRMSAALDFGCVWVNDHIPIVSEMPHGGFKHSG
                     YGKDMSIFSIEHYTELKHVMIKH"
     gene            1484..>2347
                     /locus_tag="VFX21_00635"
     CDS             1484..>2347
                     /locus_tag="VFX21_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX5094479.1"
                     /translation="MQQTHAMVQTTQAPCDIAPRRFGGDRALTEQAGERDVIELERVH
                     KRFGNFVAVHDANFSIARGEFFAMLGPSGCGKTTTLRMIAGFEAPTSGVVRLDGQDVS
                     HVPPYKRNVNTVFQQYALFPHMSVWDNVAFGPRSHKMGKAEMRTRVGDMLDIVRLADF
                     SHRKPSQLSGGQQQRVALARALINYPSALLLDEPLAALDLKLRQAMQIELKRIQREVG
                     ITFVFVTHDQEEALTMSDRIAVMSDGLVEQIGSPREIYDAPATVFVAGFIGSANLFRS
                     TVSAVNGTTADV"
BASE COUNT          365 a          820 c          771 g          391 t
ORIGIN      
        1 agtgcttgat catcacgtgc ttgagctcgg tgtagtgctc gatcgagaag atcgacatgt
       61 ccttgccgta gcccgagtgc ttgaacccgc cgtgcggcat ctcggagacg atcgggatgt
      121 ggtcgttgac ccacacgcag ccgaagtcga gcgccgcgct catgcgcgtc gcccgaccga
      181 cgtcgcgggt ccacacgctc gccgacaacc cgtactccac gtcgttcgcc cacgcgagcg
      241 cctggtcgtc gtcggtgaag cgctgcacgc tgatcaccgg gccgaagacc tcgcgctgca
      301 cgatctcgct gccctgatcc gggttcacga cgaccgacgg ctcgtagaaa tagccggcgc
      361 cctcgcgcac gcggccgccg gtcgtgacct ccgcgccgcc gtcgcgcgcc cggtcgacca
      421 tgccctgcac gcgtgcgagc tgctcgcccg agatcacggg gcccatcccg gtgttctcgt
      481 cgaagggatc gcccgtcgcg atcttgccga cggaatcggt caggtcactg accagcgtgt
      541 cgaacacgcc cgcgccggcc agcacccggc acggggcagt gcagtcctgc cccgagttgt
      601 agtagccggt ctcggtgagc accgcgacga gcgcttcgac atcggcgtcg tcgaacacga
      661 ccaccggcgc cttcccgccc agctcgaggt gcactcgctt gagcgaatcg gcagccgcgc
      721 gcgcgatcgc cttgccggtt gcgacgtcac cggtcagcga gaccatcgcg acatcggggt
      781 gcgcgacgag cgcggcgccg gtgtgctcgc cgtctccggt cacgacgttg agcacgcccg
      841 gcggcaggat gtccgcggtg atctccgcga gccggagcgc ggtcagcggc gtcagctccg
      901 acggcttcag cacgacggtg ttgccggccg cgagcgcggg gccgagcttc cacgtcgcca
      961 tgttgagcgg gtagttccac ggtgcgatcg acgccaccac gccgagcggg tcgcggcgca
     1021 cgaacgacgt gtggtcttcc atgtactcgc cggccgcgcg tccttcgagg aaccgcgcgc
     1081 cggcggcgaa gaagcgccag ttgtcgacgg tcaggtccat ctcgaactcg atgatcgaca
     1141 acggcttgcc gacgttcttg atctccagtt cgcgaatcgt cgccatgtcg gtctcgatcg
     1201 cgtcggcgac cttggtcagt gcctcgaagc gttggcgcgg ggtggtctgc gaccaggcct
     1261 cgaaggcacc ggccgcggcg gccacggccg cgtcgacgtc ggccgggccg ctcgccgcga
     1321 cctcgtcgag cacctcaccc gttgccgggt tcaggacctt gtcggtcgcg cccgcctgcg
     1381 cgccctgcca cttgccgccg atgtagtttt cgcgagccat cttgcggcct cctgaacgga
     1441 ttccgttgtt gagctgggtc catcgtacgg attcccttgc ggagtgcaac agacccatgc
     1501 gatggtgcaa acgacgcaag ccccgtgcga tattgcgccg cgtcgatttg ggggtgatag
     1561 ggcgctgacc gaacaagccg gcgagcggga tgtcatcgag ctggagcgcg tgcacaagcg
     1621 cttcgggaac ttcgtcgcgg tccacgacgc caacttctcg atcgcccgcg gcgagttctt
     1681 cgcgatgctc ggcccgtcgg ggtgcggcaa gacgaccacc ttgcggatga tcgcgggctt
     1741 cgaggcgccg acgtccggcg tcgtgcgcct cgacggccaa gacgtgtcgc acgtgccgcc
     1801 ctacaagcgc aacgtcaaca cggtcttcca gcagtacgcg ctgttcccgc acatgtcggt
     1861 gtgggacaac gtcgcgttcg ggccgcgctc gcacaagatg ggcaaggccg agatgcgtac
     1921 gcgcgtcggc gacatgctcg acatcgttcg cctcgccgac ttctcgcatc gcaagccgtc
     1981 gcagctctcg ggcggtcagc aacagcgcgt cgcgctcgca cgcgcgctga tcaattaccc
     2041 cagcgcgctg ttgctcgacg aaccgctcgc ggcgctcgac ctcaagctgc gccaagcgat
     2101 gcagatcgag ttgaaacgca tccagcgcga ggtcggcatc acgttcgtgt tcgtcacgca
     2161 cgatcaagaa gaagcactca cgatgagcga ccgcatcgcg gtcatgagcg acgggctcgt
     2221 cgagcagatc ggctcaccgc gggagatcta cgacgcgccc gcgaccgtct tcgtcgccgg
     2281 cttcatcggg agcgccaacc tcttccggtc cacggtgagc gcggtgaacg gcacgaccgc
     2341 ggacgtc
//
LOCUS       DASUGA010000034         2071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_546690, whole genome shotgun sequence.
ACCESSION   DASUGA010000034 DASUGA010000000
VERSION     DASUGA010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546690"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VFX21_00640"
     CDS             <1..966
                     /locus_tag="VFX21_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5094480.1"
                     /translation="VHGAVTGTTGDDVYLNSGPMFHLGTLMHTFSTLVAGGTNVFVRR
                     NEPAELARIISEERCTGAFLVGPMVEGLLEINADGAYDLSSLRFAPGRAEWDAMVSPD
                     TSRWAANPGGYGQTETVGFMTFNIFGLDALGTHGRTLPWLQVRIVDPDGNELPAGETG
                     EIVARGVTVMNEYWNRPEENARRQRDGWHHSHDLGRREADGSLTFVGPATRMIKSAAE
                     NIYPAEVEACIRAHPEVADCAVIGIPDPKWVQSVKAIVVRAPNSSIDADVVIEHCRAR
                     IASYKKPRTVEFVEALPRQGFLVDYDALDAAFGGGNYPGGRVRSA"
     gene            963..1814
                     /locus_tag="VFX21_00645"
     CDS             963..1814
                     /locus_tag="VFX21_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094481.1"
                     /translation="MTDIAEPCSATDRSGTGFTDTGDPDAPTETTMHQHGSYRRRIRL
                     VNEPGLVRGGLEDDFHYFRVAVRHDGSVVTGIEVEAFRWPWTTCPDAGNQLQLLVGMP
                     LSPRCLAVGDHANPRLQCTHQFDLTGLAIAHATRDEHARQYDAEVPYGTGRDGSHDVR
                     LWRDGELALSWTIEGFACVAPEPYASVPWRGGFLRWADEHLDAETAEAAIVLRRACDI
                     SFGRGANLEGVRVASELGIAGSGVCYTFQEDTARVAFRQVGTIRDFHDRVDDLLAEGP
                     HAQGDRA"
     gene            1811..>2071
                     /locus_tag="VFX21_00650"
     CDS             1811..>2071
                     /locus_tag="VFX21_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005199165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5094482.1"
                     /translation="MNLTMLLDMAAAGFDDRTAFGTRDHGVTYTELLARAQVGAAQLR
                     ELGVEHLVYVATNGPEFPVALFAAARAEIPLVPVNYRLGHDQL"
BASE COUNT          321 a          761 c          697 g          292 t
ORIGIN      
        1 gtgcacggcg cggtcaccgg caccaccggc gacgacgtgt acctcaacag cgggccgatg
       61 ttccacctcg gcacactgat gcacaccttc tcgacgctcg tcgccggcgg caccaacgtc
      121 ttcgtgcgcc gcaacgaacc cgccgagctg gcgcgcatca tcagcgagga acggtgcacc
      181 ggcgccttcc tcgtcggccc gatggtggaa gggctgctcg agatcaacgc cgacggcgcc
      241 tacgacctgt cgagcctgcg cttcgccccg gggcgagccg agtgggacgc gatggtgagc
      301 ccggacacga gccgctgggc cgcgaacccc ggcggttacg gccagaccga gaccgtcggc
      361 ttcatgacgt tcaacatctt cggtctcgat gcactgggca cgcacggccg cacgttgccg
      421 tggctccagg tccgcatcgt cgaccccgac ggcaacgagc tgcccgcggg cgagaccggt
      481 gagatcgtcg cgcgtggcgt caccgtgatg aacgagtact ggaaccggcc cgaggagaac
      541 gcgcgccgac agcgcgacgg ctggcaccac tcgcacgacc tcggtcgccg tgaagccgac
      601 ggctcgctca cgttcgtcgg gcccgcgaca cgcatgatca agtcggcggc cgagaacatc
      661 taccccgccg aggtggaggc gtgcatccgc gcgcaccccg aggtggccga ctgcgcggtg
      721 atcggcatcc ccgacccgaa gtgggtacag agcgtgaagg cgatcgtggt gcgcgccccc
      781 aactcctcga tcgacgccga cgtcgtgatc gagcactgcc gcgcgcgcat cgcgtcgtac
      841 aagaagcctc gcacggtcga gttcgtcgaa gcgctcccgc gccagggatt cttggtggac
      901 tacgacgcgc tcgacgccgc cttcggtggc ggcaactacc ccggaggacg ggtccgatcc
      961 gcatgaccga catcgccgag ccctgctccg ccaccgatcg ctccggcacc ggcttcaccg
     1021 ataccggcga ccccgacgcg ccgaccgaga cgacgatgca ccagcacggg tcgtaccggc
     1081 ggcgcatccg cctcgtgaac gagcccggcc tcgtgcgcgg cgggctcgag gacgacttcc
     1141 actattttcg ggtcgcggtg cgccacgacg gatcggtcgt caccggcatc gaggtggagg
     1201 cgttccggtg gccctggacc acttgccccg atgccggcaa ccagctccag ctgctcgtcg
     1261 gcatgccgct gtcgccgcgg tgcctcgcgg tgggtgacca cgcgaacccg cgactgcagt
     1321 gcacgcacca gttcgacctc accggcctcg cgatcgcgca cgcaacgcgc gacgagcacg
     1381 cacggcagta cgacgccgag gtgccgtacg gcacgggacg cgacggatcg cacgacgttc
     1441 gcctctggcg tgacggcgag ctcgcgctgt cgtggacgat cgaggggttc gcatgcgtgg
     1501 cgcccgagcc ctacgcatcg gtgccatggc gcggcggctt cctgcggtgg gccgacgagc
     1561 acctcgacgc cgagactgcc gaggccgcga tcgtgctgcg ccgtgcctgc gacatctcgt
     1621 tcgggcgcgg cgcgaacctg gaaggcgtgc gcgtcgcgtc cgagctcggg atcgccggca
     1681 gtggcgtgtg ctacacgttc caggaggaca ccgcccgagt cgcgttccgt caggtcggca
     1741 cgatccgcga tttccacgac cgtgtcgacg acctcctcgc agaggggccc cacgcccaag
     1801 gagatcgggc atgaacctca cgatgctcct cgacatggcg gccgccgggt tcgacgatcg
     1861 caccgcgttc gggacgcgcg atcacggcgt cacgtacacc gagctcctcg cgcgcgcgca
     1921 ggtcggcgcc gcgcagctcc gtgagctcgg ggtcgagcac ctcgtgtacg tcgccaccaa
     1981 cggcccggag ttccccgtcg cgctcttcgc cgccgcgcgc gccgagatcc cgctggtgcc
     2041 ggtcaactat cgcctcggcc acgaccagct g
//
LOCUS       DASUGA010000035         8572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_126697, whole genome shotgun sequence.
ACCESSION   DASUGA010000035 DASUGA010000000
VERSION     DASUGA010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126697"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VFX21_00655"
     CDS             <1..621
                     /locus_tag="VFX21_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HEX5094483.1"
                     /translation="ASYNSDPTVDVFLVQVPLPQQLDEEATLLAVDPAKDADGLHPVN
                     LGKLVMGARAPRPCTPSGIQALLVHYGVPIEGQHVVIVGRGLTIGRPLANLLSLKEPN
                     ANATVTVCHTRTHDLAAITRQADIIVAAAGVPSIITPDMVRPGAAVVGAGMTMEGRRV
                     VPDVDEACAEVAGWITPRLGGVGPTTRAILLQHAVEAAERRAGAAR"
     gene            618..827
                     /locus_tag="VFX21_00660"
     CDS             618..827
                     /locus_tag="VFX21_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094484.1"
                     /translation="MSAQPLPASPRCTVCGTEVEADVARCPSCGLHRPTATGPRVLAR
                     SWVWALGLLLLVVWVATLAVVAGAK"
     gene            958..1683
                     /locus_tag="VFX21_00665"
     CDS             958..1683
                     /locus_tag="VFX21_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX5094485.1"
                     /translation="MALETAYLPTSGPAHGSSLLAAVVATHLVGVDEEQRELLPRFID
                     QARDGLTIPRIALRHRLQNDKHGLDRSRHRVVGEEGLLVVEVDVHGAATPQVLGAIMG
                     AASLPPSGRGIALDTVARVVNGRWPGLSSDVLVRAVWDRSGIAPPPLAGTRDWDRSTP
                     PEERQWDGISVERRWAMEVFGLQAGMEVERDEISRRFRRLLRDAHPDHGGAAAKAATR
                     IAELTEARAILLDAASAEVAAEG"
     gene            1724..2710
                     /locus_tag="VFX21_00670"
     CDS             1724..2710
                     /locus_tag="VFX21_00670"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX5094486.1"
                     /translation="MSDPVRVAVTGAAGQIGYSLLFRIANGDLLGKDQPIILHLLEIT
                     PALPALGGVVMELDDCAFPLLRDVVTTDDANAALDGVNYALLVGSRPRTKGMERGELL
                     EANGAIFTTQGKALAKGAASDVRVLVVGNPANTNCLIAMNNATGIPRERFTAMMRLDH
                     NRAKAQLATKCGVPITSVTNMTIWGNHSATQYPDLFHAKVDGKTASGLVDRQWIEETY
                     IPTVQQRGAAIIEARGASSAASAANAAIDHMRDFALGSAANDWVSMGICSDGSYGVPE
                     GLIAGFPCTTKNGDWSIVQGLDIDDFSRGRIDASVAELADERDAVAELGLLG"
     gene            2758..3189
                     /locus_tag="VFX21_00675"
     CDS             2758..3189
                     /locus_tag="VFX21_00675"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX5094487.1"
                     /translation="MSEQGVCAIGLPADDLAELVADATRKKDAEVARALDDFFEGRSP
                     TLTVPLDLDGIDGFRRDVLETLAREVGWGETVSYGELAAMAGRPGAARAVGTTMANNP
                     LPFVIPCHRVIAAGGKIGGYGGGRNAVELKRKLLAREGVYL"
     gene            complement(3237..4523)
                     /locus_tag="VFX21_00680"
     CDS             complement(3237..4523)
                     /locus_tag="VFX21_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5094488.1"
                     /translation="MSVQATRASEPTAVLAHIEDGADLVVPLANGEPRTLIDAIDAAA
                     ESLEGVRVRQMHALHEHPYLHGVYGDKLRHVAYFLSAVTRKAFAEGGCDLVPANFSEV
                     PLLLRKTTKCSLVLAAASPPDRHGYFSLGTNADYTARFIGQVPFFLEVNPNMPRTFGE
                     NSIHVSQIEGWVEADYPLIEVAPPPASEKDRAIAALVAERVPDGATIQAGIGAIPNAI
                     LGMLTERRNLGIHTELISDGVMDLMDSGAITGTNKVTRPGKITATFALGTQRLYDFLH
                     ENPAVEFLPVDWLNDPRVIGREHAFVSINATVEVDLLGQCNSEMIAGRYYSGSGGQAD
                     FARGAMYSEHGLGFVVLHSTTADEQTPRIVAQLCPGAPVTTMKNTVDHVVTEYGVAEL
                     RGRSIAERARDLIAVAHPKFRDQLTAEAKALGYLRD"
     gene            complement(4520..6154)
                     /locus_tag="VFX21_00685"
     CDS             complement(4520..6154)
                     /locus_tag="VFX21_00685"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX5094489.1"
                     /translation="MVEFAYQDILPLGADETPYRLLTSDYVSTFEAGGRTFLQVEPEA
                     LTMLTAQAMFDIAHLLRPGHLAQLRAILDDAEASPNDHFVALELLKNACIAAGGVLPS
                     CQDTGTALVKGKKGELVLTGGGDREAIARGIFRTYEQDNLRYSQMAPIDTYTEKNTGT
                     NLPAEIDIDAVDGDAYKLLFMAKGGGSANKSLLFQETKALLNPQSLMSWLDEKLRTLG
                     TAACPPYHLALVIGGTSAEFALKTAKLASARYLDTLPRDGNELGRGFRDVELEQDVLK
                     LTQEFGIGAQFGGKYFCHDVRVIRLPRHGASCPVALAVSCSADRQSLGKITRDGVFLE
                     ELETDPAKYLPEVTEDALEGGPAVAIDLNRPMDEIRNTLSQYPVKTRVMLTGPMVVAR
                     DIAHAKLKERVDAGDGLPQYMKDYCVYYAGPAKTPEGYASGSFGPTTAGRMDSYVDLF
                     QRNGGSFVMLAKGNRSKAVTDACKEYGGFYLGSIGGPAARLAKDCISNVEVLEYPELG
                     MEAVWKIEVHDFPAFVVVDDKGNDFFKDVSKPVALR"
     gene            6177..7562
                     /locus_tag="VFX21_00690"
     CDS             6177..7562
                     /locus_tag="VFX21_00690"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX5094490.1"
                     /translation="MAEDRVEHDSMGEVRVPAAALWGAQTQRAIENFPISGRPIEARL
                     VRALALIKAEAARVNAALDEVALDDATGDAIAAAADAVARGEHDGAFPIDVFQTGSGT
                     SSNMNANEVIARLASEQLDEPVHPNDDVNASQSSNDVFPSAVHIAVAEGVVEDLVPAC
                     EELARALRAKQEEFATVVKSGRTHLMDATPVTLGQEFGGYAAQVEEGVARLRDTLPRV
                     CALPLGGTAVGTGINAPPAFARAVIAQLAERTGLPLTEAPDHFAAQGARDALVECSGQ
                     LRTLAVALIKIANDIRWMGSGPRTGLAEIRLPDLQPGSSIMPGKVNPVMAEVLTQVGA
                     QVIGNDAAVAFSGSQGNFELNVFVPVMARNLLESIRILAAACRAFATKAVAGIEANVD
                     RLRAYAEASPSIGTALNPYIGYETAAEVIKESTRTGRSIREIVRERGLMTEEELDRAL
                     DVLAMTRGGIV"
     gene            7563..8498
                     /locus_tag="VFX21_00695"
     CDS             7563..8498
                     /locus_tag="VFX21_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003985904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX5094491.1"
                     /translation="MAGHEGSKKAVIAALLANLAIAISKFVVYLITGAASLLAEAIHS
                     FADTGNQGLLLYGGKAAQKPPDDEHPFGYGNRRYFWAFIVALVLFSLGGLFAIYEGIE
                     KLRHPHELESVGLAIALLIFALIVEGLSFRTAIHEANKARGKQHLFRFIHRTKAPELP
                     VVLLEDLGAMVGLLIALFGVTMAHFTDEPRWDAAGSLAIGILLVIIAIFLAVEMSSLL
                     LGEAATTEDVAKLRAAIDEHPTVGCIIHLRTEHIAPDEIVVATKLEFDHSLSFEQLAD
                     EINGVEARMRAAVPAARIIFIEPDVYRPEAAAAAD"
BASE COUNT         1320 a         2850 c         3018 g         1384 t
ORIGIN      
        1 gcgtcgtaca acagcgaccc gaccgtcgac gtattcctcg tgcaggtgcc gctgccgcag
       61 cagctcgatg aagaggcaac gctgctcgcg gtcgatcccg cgaaggacgc cgacggcctg
      121 caccccgtga acctcggcaa gctcgtcatg ggcgctcgtg caccgcgtcc gtgcacgccg
      181 agcggtatcc aggcgttgct cgtgcactac ggcgtgccga tcgaaggcca gcacgtggtc
      241 atcgtcgggc gtggtctgac gatcgggagg ccgctcgcga acctgttgtc gctgaaggag
      301 ccgaacgcca acgcgacggt gacggtgtgc cacacgcgca ctcacgacct cgcggcgatc
      361 acccgtcagg ctgacatcat cgtcgcggcg gcgggcgtgc cgtcgatcat cacgcccgac
      421 atggtgcgac ccggtgctgc ggtcgtcggc gcgggcatga ccatggaagg ccgccgggtc
      481 gttcccgacg tcgacgaggc ctgcgccgag gtcgccggtt ggatcacgcc gcgcctgggc
      541 ggtgtcggcc cgacgacgag agcgatcttg ttgcaacacg cggtcgaggc ggccgagcgg
      601 cgggcgggag cagcccgatg agcgcgcagc cgctgcccgc gtcgccgcga tgcaccgtgt
      661 gcggcaccga agtcgaagcc gacgtcgcgc gctgcccctc gtgtggtctg caccggccga
      721 ccgcgacggg cccgcgggtg ctggcgcgca gttgggtctg ggcgctgggg ctgctgctcc
      781 tcgtcgtctg ggtcgccacg ctggccgtcg tcgcgggcgc caagtagcgc cgactcggca
      841 cgcacgcggg ccgtccgaca agatgggcgg taccgaagac gggtgggagg ccgaaacccg
      901 ttgctactcg ccgagctcgt cgtccgtcac acccgtcggc acatgccgac ccggcgcgtg
      961 gctctcgaga ccgcgtacct gccgacatcg ggtcccgcgc acggcagctc gttgttggcc
     1021 gctgtcgtcg cgacgcacct cgtaggcgtc gacgaggagc agcgtgagtt gttgccccgc
     1081 ttcatcgacc aagcgcgtga cggcttgacg atcccgcgca ttgcactgcg gcaccggttg
     1141 cagaacgaca agcacggcct cgaccgctct cgccaccgtg tcgtcggcga agagggactc
     1201 ctggtggtcg aagtcgacgt gcacggcgcg gcaacgccgc aagtgctcgg tgcgatcatg
     1261 ggtgccgcgt cgttgccgcc gtccggccgt gggatcgcgc tcgacaccgt cgcgcgcgtc
     1321 gtgaacggac ggtggccggg cctgtcgtcc gacgtgctcg tacgcgcggt ctgggatcgc
     1381 agcggcatcg cgccgccgcc gttggccggg acgcgtgact gggaccgcag cacgccgccc
     1441 gaggagcgcc agtgggacgg gatctcggtc gaacggcgct gggcgatgga agtgttcggg
     1501 ctgcaggccg gcatggaggt cgagcgcgac gagatcagtc gccgctttcg gcgcctcttg
     1561 cgagacgccc accctgatca cggcggtgcg gccgcgaagg ccgcgacgcg cattgccgag
     1621 ctgaccgaag cgcgggcgat tctcctcgat gcggcttcgg cggaagtcgc cgcggagggc
     1681 tagcgtcaaa acggcatttc gctcggtatc gaaggagttc cgtgtgtcag atcccgtccg
     1741 cgtcgcggtg accggtgcgg ccggtcagat cggctacagc ctgctgttcc gtatcgccaa
     1801 cggcgacctg ctcggcaagg accagccgat catcctgcac ctgctcgaga tcacacccgc
     1861 gctgcccgcg ctcggcggcg tcgtcatgga gctcgacgac tgcgcgttcc cgctgctgcg
     1921 agacgtcgtc accaccgacg acgcgaacgc ggcgctcgac ggtgtgaact acgcgctgct
     1981 ggtcggttcc cgcccccgca ccaagggcat ggaacgcggt gagctgctcg aggccaacgg
     2041 cgccatcttc accacgcagg gcaaggcgtt ggccaagggt gccgcgagcg acgtccgtgt
     2101 cctcgtggtg ggcaaccccg ccaacacgaa ctgcttgatc gcgatgaaca acgcgacggg
     2161 gatcccgcgc gagcggttca cagcgatgat gcgccttgat cacaaccggg ccaaggcgca
     2221 gctcgcgacg aagtgcggcg tgccgatcac gtcggtcacg aacatgacga tctggggcaa
     2281 ccactccgcg acgcagtacc ccgacctctt ccacgccaag gtcgacggca agacggcatc
     2341 gggtctcgtc gaccggcagt ggatcgaaga gacgtacatc ccgaccgtgc aacagcgcgg
     2401 cgcggccatc atcgaggcgc gcggcgcgtc gtcggcggcc tcggcggcga acgcggcgat
     2461 cgatcacatg cgcgacttcg cgctcggctc ggccgcgaac gactgggtca gcatgggcat
     2521 ctgctccgac ggttcctacg gcgtgcccga gggcctcatc gcggggttcc cgtgcaccac
     2581 gaagaacggc gattggtcga tcgtccaagg cctcgacatc gacgacttct cgcggggccg
     2641 catcgacgcc tccgtcgccg agctcgccga cgagcgcgac gcggtcgccg agttggggct
     2701 gctcggctga tgcagtactg gcggcagtcg agcccgcacg ggcaagtgac tgttgtcatg
     2761 agcgagcagg gcgtgtgcgc gatcggcttg cccgcggacg acctcgcgga gctggtcgcc
     2821 gacgcgaccc gcaagaagga cgcagaggtc gcgcgcgccc tcgacgactt cttcgaaggg
     2881 cgttcaccga cgctgacagt gccgctcgac ctcgacggca tcgacggctt ccgtcgtgac
     2941 gtgctcgaga cgctggcgcg cgaggtcgga tggggcgaga ccgtgtccta cggcgagctg
     3001 gcggcgatgg ccggccgccc cggcgcggcg cgcgcggtcg gcacgacgat ggccaacaac
     3061 ccgctgccgt tcgtgatccc gtgccaccgg gtgatcgcgg ccggcggcaa gatcggcggc
     3121 tacggcggcg gccgcaacgc ggtcgagctc aagcgcaagc tcctcgcccg cgaaggcgtg
     3181 tacctataac tcgggcgtcg ctcagcccca atgattgtgg gtcagcgacg cccgagtcag
     3241 tcgcgcaagt agccgagggc cttggcctcg gcggtgagct gatcgcggaa cttcgggtgc
     3301 gcgaccgcga tgaggtcgcg tgctcgctcc gcgatcgagc ggccccgcag ctcggcgacg
     3361 ccgtattcgg tgacgacgtg gtcgaccgtg ttcttcatcg tcgtcaccgg cgcacccggg
     3421 cacagctgcg caacgatgcg cggcgtctgc tcgtcggccg tcgtcgagtg cagcacgacg
     3481 aagccgagcc cgtgctcgct gtacatcgcc ccgcgcgcga agtcggcttg gccgccgctg
     3541 ccggagtagt agcgacccgc aatcatctcg gagttgcact ggccgaggag gtcgacctcg
     3601 accgtcgcgt tgatcgacac gaaggcgtgc tcccgaccga tgacgcgcgg atcgttgagc
     3661 cagtcgacgg gcaggaactc cacggcgggg ttctcgtgca ggaagtcgta gaggcgctgc
     3721 gtgccgagcg cgaaggtcgc tgtgatcttg cccggtcgcg tcaccttgtt ggtgccggtg
     3781 atcgcgccgg agtccatgag atccatgacg ccgtcggaga tcagctcggt gtggataccg
     3841 aggttgcggc gctcggtcag catcccgagg atcgcgttcg ggatcgcgcc gatgccggct
     3901 tggatcgtcg cgccgtcggg cacgcgctcg gcgacgagcg cggcgatcgc gcgatccttc
     3961 tcgctcgcgg gcggcggcgc gacttcgatg agcgggtagt cggcctcgac ccagccctcg
     4021 atctggctca cgtggatcga gttctcaccg aacgtgcgcg gcatgttcgg gttcacttcg
     4081 aggaagaacg gcacctgacc gatgaagcgg gcggtatagt cggcgttggt gcccagcgag
     4141 aagtaaccgt gccggtcggg tggcgatgcc gcggccagca cgagcgagca cttcgtcgtc
     4201 ttgcgcaaca gcagcgggac ttcgctgaag ttcgcgggga cgaggtcgca gcctccttcc
     4261 gcgaacgcct tccgggtgac tgcgctcagg aagtacgcga cgtgccgcag cttgtcgccg
     4321 tagacgccgt gcaggtacgg atgctcgtgc aacgcgtgca tctggcgcac gcgcacgcct
     4381 tcgagcgact cggccgcggc atcgatggcg tcgatcagcg tgcgcggctc tccgttcgcg
     4441 agcgggacga cgagatccgc gccgtcttcg atgtgcgcga ggaccgcggt cggttccgac
     4501 gcccgcgtcg cctgcacgct caccgcagtg cgaccggctt ggagacgtcc ttgaagaagt
     4561 cgttgccctt gtcgtcgacg acgacgaacg cgggaaagtc gtgcacctcg atcttccaca
     4621 cggcttccat gccgagctcg gggtactcga gcacttcgac gttggagatg cagtccttgg
     4681 cgaggcgcgc cgcggggccg ccgatcgagc cgaggtagaa cccgccgtac tccttgcacg
     4741 cgtcggtgac tgccttcgac cggttgccct tggcgagcat gacgaacgac ccgccgttgc
     4801 gctggaacaa gtcgacgtag gaatccatgc gccccgcggt cgtcgggccg aacgatcccg
     4861 atgcatagcc ctcgggtgtc ttcgccggtc ccgcgtagta cacgcagtag tccttcatgt
     4921 actgcggcag cccgtcgccc gcgtcgacgc gctccttgag cttcgcgtgc gcgatgtcgc
     4981 gcgcgacgac catcgggccg gtcagcatca ctcgggtctt cacgggatac tgcgagagcg
     5041 tgttgcggat ctcgtccatc gggcggttca ggtcgatcgc gacggcgggc ccgccttcga
     5101 gcgcgtcctc ggtgacctcg gggaggtact tcgcgggatc ggtctccagc tcttcgagga
     5161 acacgccgtc gcgcgtgatc ttgccgagcg actgccggtc ggccgaacac gacacggcca
     5221 gtgccaccgg gcacgacgcg ccgtgacggg gcaggcggat gacgcgcacg tcgtggcaga
     5281 agtacttgcc gccgaactgc gcgccgatac cgaactcctg cgtgagcttc agcacgtctt
     5341 gttcgagctc gacgtcgcgg aagccgcgac cgagctcgtt gccgtcacgg gggagtgtgt
     5401 cgaggtagcg cgcggacgcg agcttcgcgg tcttcagcgc gaactcggcc gacgtaccgc
     5461 cgatgaccag cgcgaggtgg tacggcgggc acgcggcggt gccgagcgtg cgtagcttct
     5521 cgtcgagcca gctcatgagg ctctgcgggt tgagcagcgc cttcgtctcc tggaacagga
     5581 ggctcttgtt cgcggagccg ccgcccttgg ccatgaacag cagcttgtac gcgtcgccgt
     5641 cgaccgcgtc gatgtcgatc tcggccggca ggttcgtgcc ggtgttcttc tcggtgtacg
     5701 tgtcgattgg cgccatctgc gagtagcgca ggttgtcttg ctcgtaggtg cggaagatgc
     5761 cgcgcgcaat cgcttcgcgg tcgccaccac cggtgagcac cagttcgccc ttcttgccct
     5821 tgacgagcgc ggtgccggtg tcctggcacg acggcagcac gccgccggcc gcgatgcacg
     5881 cgttcttcaa cagttcgagc gcgacgaaat gatcgttcgg tgacgcctcg gcgtcgtcga
     5941 ggatcgcccg caactgtgcg aggtgcccgg gccgcagcaa gtgcgcgatg tcgaacatcg
     6001 cctgcgcggt gagcatcgtc agtgcttccg gctcgacctg caggaacgtt cggcctccgg
     6061 cttcgaacgt cgacacgtag tcgctcgtca gcaagcggta cggcgtctcg tcggcgccga
     6121 ggggaaggat gtcctggtag gcgaattcca ccatggctgc aagttaccgt tgacgcatgg
     6181 ccgaagaccg tgtcgagcac gattccatgg gcgaggtccg cgtcccggcg gccgcgttgt
     6241 ggggcgcgca gacacagcgc gcgatcgaga actttcccat ctcgggacga cccatcgagg
     6301 cgcggctcgt acgcgcgctg gcgttgatca aggccgaggc ggcgcgcgtc aacgcggcgc
     6361 tcgacgaagt cgcgctcgac gacgcgaccg gtgacgcgat cgcggcggcg gcggacgcgg
     6421 tggcgagagg cgagcacgac ggcgccttcc cgatcgacgt gttccaaacc ggctccggca
     6481 cctcgtcgaa catgaacgcg aacgaggtca tcgcgcggct ggcatcggag cagttggatg
     6541 aaccggtgca cccgaacgac gatgtgaacg cgtcgcagtc gtccaacgac gtgttcccgt
     6601 cggccgtgca catcgcggtt gccgaaggcg tggtcgagga tctcgtgccg gcgtgcgagg
     6661 agctggcgcg cgcgttgcgg gccaagcagg aggagttcgc gacagtcgtg aagtcggggc
     6721 ggacgcacct gatggacgcc acgccggtca ccctcggcca ggagttcggc ggttacgccg
     6781 cgcaagtcga agagggcgtg gcgcggttgc gtgacacgtt gccacgcgtg tgcgcgttgc
     6841 cgctgggcgg aacggccgtg ggtaccggca tcaacgcgcc gcccgcgttc gcgcgcgcag
     6901 tgatcgcgca gctggccgag cgcaccggat tgcccttgac cgaagcgccc gaccacttcg
     6961 ccgcgcaggg cgcacgcgac gcgctcgtcg aatgcagcgg gcagctgcgc acgctggcgg
     7021 tcgcgttgat caagatcgcc aacgacatcc gttggatggg cagtggcccg cgtacgggtc
     7081 tcgccgagat ccggttgccc gacctccaac ccggctcgtc gatcatgccc ggcaaggtga
     7141 acccggtgat ggccgaagtg ctgacccagg tcggcgcgca ggtcatcggc aacgatgccg
     7201 cggtcgcgtt ctcggggtcg cagggcaact tcgagctgaa cgtgttcgtg cccgtcatgg
     7261 cccgcaacct gctcgagtcg atccggatcc tcgccgccgc gtgccgcgca ttcgcgacga
     7321 aggcggtcgc gggcatcgaa gccaatgtcg accggctccg tgcgtacgcg gaagcgtcac
     7381 cgtcgatcgg tacggcgctg aacccgtaca tcggctatga aacggcggcc gaagtcatca
     7441 aggagtccac gcggaccggg cggtcgatcc gcgagatcgt gagggagcgg ggattgatga
     7501 ccgaagagga gctcgaccgg gcgctcgatg tgctcgcgat gacgcgcggg gggatcgtgt
     7561 agttggccgg gcacgaaggt tcgaagaaag ccgtcatcgc cgcgctgctg gcgaacctcg
     7621 cgatcgcgat aagcaaattc gtcgtctacc tgatcacggg cgcggcgtcg ctgctggcgg
     7681 aagcgatcca ctcgttcgcg gacaccggta accaaggatt gttgttgtac ggcggcaagg
     7741 ccgcgcagaa gccgcccgac gacgagcacc cgttcgggta cggcaaccgg cgctacttct
     7801 gggcgttcat cgtcgcactg gtgctgttca gcctcggtgg gctgttcgcg atctacgagg
     7861 gcatcgagaa gctgcgccac ccgcacgagc tggagagcgt cgggctcgcg atcgcgttgc
     7921 tgatctttgc gctcatcgtc gaagggctct cgttccgcac tgcgatccac gaagcgaaca
     7981 aggcgcgcgg gaagcagcac ctgttccggt tcatccaccg cacgaaggcg cccgagcttc
     8041 cggtcgtcct gctcgaagac ctgggcgcga tggtcggtct cctgatcgcg ctgttcggcg
     8101 tcacgatggc gcacttcacc gacgaaccac gctgggacgc ggcgggcagc ctcgcgatcg
     8161 gcatcctgct cgtgatcatc gcgatcttcc tcgcggtcga gatgtcgagc ctcctgctcg
     8221 gcgaggccgc gacgaccgag gacgtcgcga agctgcgcgc cgcgatcgac gagcacccga
     8281 cggtcggctg catcatccac ctgcgcaccg agcacatcgc gcccgacgag atcgtggtcg
     8341 caaccaagct cgagttcgac cactcgctct cgttcgagca gctggccgac gagatcaacg
     8401 gcgtcgaagc gagaatgcgc gccgcggtcc ccgcggcccg catcatcttc atcgaacccg
     8461 acgtctaccg ccccgaagcc gccgccgcgg ccgattaagc gggggtttcg ccgctgccgc
     8521 ccgtggccgg ctgttgcgcg ggttgttccg cggcggtcgg cgtgggttcg gg
//
LOCUS       DASUGA010000036         2630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1933349, whole genome shotgun sequence.
ACCESSION   DASUGA010000036 DASUGA010000000
VERSION     DASUGA010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1933349"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2226)
                     /locus_tag="VFX21_00700"
     CDS             complement(<1..2226)
                     /locus_tag="VFX21_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094492.1"
                     /translation="MTIDTTAAPPVQIDERQRSFLGRYAYLLLSAFVVVLMLGWTFNE
                     RWTSPDFWVHLAAVREFAHDPLHPANPLVIGGDPDPYLSPYAFVLGLVTRATGIDAIT
                     ALAVAGMLSLVALLYALAKFVRTVSAATYAPVFLLAATLVAWGVNPWRWSGFFELNSL
                     GAVLPLSSTIASALGLLTVVATHAWMQRGRAPQLVLVGAGAVLVPLCHPMTAVWVGGL
                     VAAFVVSDATRVNLRRVIALAVVGVGAVVPVALWPFFPALRLLGASDTFDASNTAMYS
                     SLATRLFLAVPGVVVLAVRARRRWRDPLVLGALINGAIYAAGFVSDHRVFGRVLPGIV
                     LVVHTAIAVWLAEVAERWNALDRRRQLAVRSGVIAVVVIGILGCTAGLVRPVPRGLLP
                     SRYADDERLASLVDPYEPVRGLIDRDDVVVASPDLALGVAASSGRVIAPPAPAPFVDD
                     VEQRRRAVAGLLSPETSATAREQLIRRYGVDWFVVPPSEVAELTAGENRALFRERTRS
                     RDLAVLETVRAPSGSTPEPAPAPTPTPTPTPTAPTAPVDEDAGTTEPTGPVRRVPDAI
                     ASDCSVDVASQLSDWIDAAPRGSRLELAPDGCYRIDGTVALENKRDILLDGKGATLEA
                     KTTGDRTRSHLKLERCVNVIVRNLTVHGANPDAGATRAAYHEDLEAQHAFELLGVRNV
                     LLDAVQATDTYGDFVYVGNSRSRDSEHVAVTRSTFARSGRQGMSVTDGTDVAIVGNDI
                     SD"
     gene            complement(2223..>2630)
                     /locus_tag="VFX21_00705"
     CDS             complement(2223..>2630)
                     /locus_tag="VFX21_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094493.1"
                     /translation="VLRQGENRDSDWQDVQHFVAANAEPGDATLFVPASNAAPFEYYE
                     LAGAGGAPNPVLPMGRWGDLEADLFDIDEARAIDRGLEAVQQHDRVWLLSGGFVREED
                     AQHVEDGLQRFGPPAVDRRFGDVRARLFLDTSS"
BASE COUNT          457 a          883 c          929 g          361 t
ORIGIN      
        1 gtcgctgatg tcgttgccga cgatcgcgac atcagtgccg tcggtgacgg acatcccttg
       61 gcggccactg cgcgcaaagg tcgatcgcgt cacggcgacg tgctcgctgt cgcggctgcg
      121 actgttgccg acgtagacga agtcgccgta cgtatccgtt gcctgcacgg cgtcgaggag
      181 gacgttgcgc acgcccagga gctcgaacgc gtgctgcgct tcgaggtcct cgtggtaggc
      241 cgctcgggtc gcgccggcat ccggattggc gccgtgaacg gtcaggttgc gcacgatgac
      301 gttcacgcaa cgttcgagct tgagatgcga ccgggtgcgg tcacccgtgg tcttcgcctc
      361 gagcgtcgcg cccttgccgt cgaggaggat gtcgcgcttg ttctcgagcg caacggtgcc
      421 gtcgatccgg tagcagccgt cgggtgccag ctcgagtcgc gagccgcgcg gagcagcgtc
      481 gatccagtcc gagagttgcg acgcgacgtc gacggagcag tcggacgcga tcgcgtcggg
      541 cacccgccgc accggacccg tcggctcggt cgtgcccgcg tcttcgtcga cgggcgcggt
      601 cggagccgtc ggcgtgggcg tgggcgtagg cgtgggtgcg ggtgcgggtt ccggtgtcga
      661 gccggatggc gcgcggaccg tctcgaggac cgcgagatca cgcgatctcg tgcgttcgcg
      721 gaacagcgca cggttctccc cggccgtcaa ctcggcaact tcggacggtg gcacgacgaa
      781 ccagtcgacg ccgtaccggc ggatcagctg ctcgcgcgcg gtcgctgacg tctccggcga
      841 gagcagcccg gcgaccgcgc gccggcgctg ctcgacgtcg tcgacgaacg gcgcgggtgc
      901 cggcggcgcg atgacacgac cgctcgacgc ggcgacaccg agcgcgagat cgggcgacgc
      961 gacgacgacg tcgtcgcggt cgatgagccc ccgcaccggt tcgtacggat cgacgagact
     1021 tgcgagtcgt tcgtcgtccg cgtagcggga tggaagcaac ccgcgcggaa ctggacggac
     1081 gagtccggca gtgcatccga ggatcccgat cacgacgacc gcgatgacac cggatctcac
     1141 ggcgagttgc cggcgccgat cgagtgcgtt ccaacgttct gcgacctcag ccagccagac
     1201 cgcgatcgcg gtgtgcacga cgagcacgat gccgggcaag acccgcccga agacgcgatg
     1261 atccgacacg aaccccgcgg catagatcgc accgttgatg agtgctccga gcacgagcgg
     1321 atctcgccac cgtcggcgcg cacggaccgc gagcacgacg acgccgggca ccgcgagaaa
     1381 caggcgggtc gcgagggagg aatacatcgc cgtgttcgag gcgtcgaacg tgtcggatgc
     1441 accgaggagg cgcagcgccg ggaagaacgg ccacagcgcc accggcacga ccgcgccaac
     1501 ccccacgacg gcgagcgcga tgacacgccg aaggttcacg cgcgtcgcgt cgctgacgac
     1561 gaacgcggcg acgagcccgc cgacccacac cgccgtcatc gggtgacaca agggcacgag
     1621 caccgcaccc gcaccgacca gcacgagctg tggcgctcgg ccacgctgca tccacgcgtg
     1681 cgtcgcgacg accgtgagca agcccagtgc cgaagcgatc gtggacgaga gtggaaggac
     1741 ggcaccgagt gagttgagct cgaagaagcc gctccatcgc cacggattca cgccccacgc
     1801 gacgagtgtc gccgcgagca ggaacaccgg cgcgtaggtc gcggcagaca cggtgcgaac
     1861 gaacttcgcc agcgcgtaca gcagcgcgac gaggctgagc atcccggcga cggcgagcgc
     1921 cgtgatcgcg tcgatgccgg tcgcgcgcgt cacgagaccg aggacgaacg cgtagggaga
     1981 caagtagggg tcgggatcgc caccgatgac gagcggattg gccggatgca acgggtcgtg
     2041 tgcgaactcg cgcactgccg cgaggtggac ccagaagtcg ggagacgtcc agcgttcgtt
     2101 gaacgtccac cccagcatca ggacgacgac gaacgcggac agcagcaggt aggcgtaccg
     2161 accgaggaac gagcgttgcc gctcgtcgat ctgcacagga ggcgcggcgg tggtgtcgat
     2221 cgtcacgacg aagtatcgag gaacagtcgg gctcggacat cgccgaatcg gcggtcgacg
     2281 gcgggcggtc cgaatcgttg cagcccgtct tcgacgtgct gcgcgtcctc ctcgcgcacg
     2341 aacccgccgc tgagcaacca cacccggtcg tgctgctgca cggcctccag cccgcggtcg
     2401 atcgcacgcg cttcgtcgat gtcgaagagg tcggcctcca gatcacccca tcggcccatc
     2461 gggagcaccg ggttcggtgc accgcccgcg ccggccagct cgtagtactc gaacggtgcc
     2521 gcgttcgagg ccggcacgaa caacgtcgca tctcccggtt cggcgttcgc ggcgacgaaa
     2581 tgctgtacgt cctgccagtc actgtcgcga ttctcgccct gtcgaagcac
//
LOCUS       DASUGA010000037         3196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1933182, whole genome shotgun sequence.
ACCESSION   DASUGA010000037 DASUGA010000000
VERSION     DASUGA010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1933182"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VFX21_00710"
     CDS             <1..361
                     /locus_tag="VFX21_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX5094494.1"
                     /translation="WGSSASPTDPNPDAFDCSELTKWAAKRVGIDLPDGSWLQYLELQ
                     EQGALVPVEQALRTPGALLFSFSDEPTPGGGRPSQAHVAISLGDGKTIEARGKKYGVG
                     TFDAGNRFEYGAIIPGL"
     gene            371..1978
                     /locus_tag="VFX21_00715"
     CDS             371..1978
                     /locus_tag="VFX21_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX5094495.1"
                     /translation="MTDYSFARAWRTVAELAPDRDAIVCGARRLTFGEFDARTERLAH
                     VLADAGLGAGDKVAIECVNAPEYLEAFLAAQKLGCVPVNVNYRYVGAELAYLLDNSDT
                     AALVFHDEFAPTVADALAMLPAVRRPRLLVQVPHDGDGTRAEGVLDYEAAIAAAPDGP
                     ATDRAPSGDDLVFLYTGGTTGYPKAVMWRSDDLYVSLWQMARPGSLPPDVRTAIERGK
                     RAATTLPACPLMHGTGLFITLSTLSGGGTVVLLDTPRLDVERLWDAVDTERVLTVTIV
                     GDAFARPMVAALDEFPNRWDLSSLRAITSSGVTWSPETKRALLAHLPGTLLIDSLGAS
                     EGIMTRTETRKGDEIAPARFKAGERIVVVDDDGELVQPGDGRIGMLGVGGAIPLGYYK
                     DDAKTAATFRTLHGRRYSIPGDYATIDADGTIQLLGRGSACINTGGEKVYPEEIELAL
                     RAHPDVFDCVVVGIPDDRWGEMVVALLELQPGATLDADALAAECRATLAGYKVPKRFL
                     AFDSLQRSPAGKADYNLLRSLATEALG"
     gene            1975..>3196
                     /locus_tag="VFX21_00720"
     CDS             1975..>3196
                     /locus_tag="VFX21_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5094496.1"
                     /translation="MIFEQYYLDCLSHASYLIADASTGRAVVVDPQRDVTQYIEDAAA
                     HNCTIERVIETHFHADFLSGHLELAAQTGAVISYGEAAGETSFPIERLADGERLSLGD
                     VVLEIRATPGHTPESISVVVYEHAGDAEPYGVLTGDTLFIGDVGRPDLLASRGMTATE
                     LARDLFRSTRTQLLTLPDATRVYPAHGAGSACGRKLSTATVSTIGEQRATNYALADMT
                     EDAFVEVVTEGLPDAPAYFAFDAQRNRDQRELLDETTAPPRLSLADVLDRVAAGAVVL
                     DTRDAADYAHGHLRDSLEVGLGGRFAEYAGDVLDPDQPIILVCDAGTEIEAKVRLARI
                     GFDNVVGVLDDHVNAFLDRPDLVDRSSRLAATELAALLEAGETIQLVDVRTADEITDG
                     AVTGAVRLPLARITS"
BASE COUNT          484 a         1149 c         1081 g          482 t
ORIGIN      
        1 gtggggttcg agcgcgtcgc cgacggatcc gaatccggac gcattcgact gctccgaact
       61 gacgaagtgg gcggcgaagc gcgtcggcat cgatctcccc gacggttcgt ggctccagta
      121 cctcgagttg caggagcagg gcgcgctcgt gcccgtcgaa caggcgctgc ggacaccggg
      181 cgcgctgttg ttctcgttct cggacgagcc gacaccgggc ggtgggcggc cttcacaggc
      241 acacgtcgcg atcagcctcg gtgacggcaa gacgatcgaa gcccgcggca agaagtacgg
      301 cgtcggtacg ttcgatgccg gcaaccggtt cgaatacgga gcgatcattc ccgggctcta
      361 acctgcccgc gtgacggact actcctttgc ccgcgcgtgg cgcaccgttg ccgagctcgc
      421 ccccgaccgt gacgccatcg tgtgcggcgc gcgccgtctg accttcggcg agttcgacgc
      481 gcgcaccgaa cgactcgcac acgtgctcgc ggacgcggga ctcggtgcgg gcgacaaggt
      541 cgcgatcgaa tgcgtcaacg cgccggagta cctcgaggcg ttcctcgcgg cgcagaagct
      601 cggctgcgtg ccggtgaacg tcaactaccg ctatgtcggc gccgagctgg cctacctgct
      661 cgacaactcc gacactgccg ccctcgtctt tcacgacgag ttcgcgccga cggtcgccga
      721 cgcactggcg atgctccccg cggtgcgccg gccgcggctg ctcgtgcagg ttccccacga
      781 cggcgacggt acgcgggccg aaggcgtcct cgactacgag gccgcgatcg cggccgcacc
      841 cgatgggccg gcgaccgatc gcgcgccgtc gggcgacgac ctcgtcttcc tgtacacggg
      901 cggcacgacc ggatatccga aggcggtcat gtggcgcagc gacgacctgt acgtgtcgct
      961 gtggcagatg gcgcgacccg gttcgctgcc acccgacgtg cgcaccgcga tcgagcgcgg
     1021 caagcgcgca gccaccacgt tgcccgcatg cccgctgatg cacggcaccg ggttgttcat
     1081 cacgctgtcg acgctgtcgg gcggcgggac cgtcgtgttg ctcgacacgc cgcgcctcga
     1141 cgtcgaacgg ttgtgggatg ccgtcgacac cgaacgcgtg ctcacggtca cgatcgtcgg
     1201 cgacgcgttc gcgcgcccga tggtcgccgc cctcgacgag ttcccaaacc gttgggatct
     1261 ctcgtcgctc agggcgatca cgtcgtcggg cgtcacgtgg agccccgaga cgaagcgggc
     1321 gctgctcgcg caccttcccg gtaccttgct catcgactcg ctcggcgcgt ccgagggcat
     1381 catgacgcgc accgagacgc gcaaaggcga cgagatcgcg cccgcgcgct tcaaggccgg
     1441 cgaacggatc gtcgtggtcg acgacgacgg cgagctcgtg cagcccggcg acggccgtat
     1501 cggcatgctg ggcgtcggcg gtgcgatccc gctcgggtac tacaaggacg atgcgaagac
     1561 cgcggcgacg ttccgcacgc tgcacgggcg gcgttattcg atccccggcg actacgcgac
     1621 gatcgacgcc gacggcacga tccagctcct cggccgtggt tcggcgtgca tcaacaccgg
     1681 cggcgagaag gtgtatcccg aggagatcga gctcgcgctg cgggctcacc ccgatgtgtt
     1741 cgactgcgtg gtcgtcggca tccccgacga ccggtggggc gagatggtcg tcgcgctgct
     1801 cgagctgcag ccgggcgcga cgctggatgc cgatgcgctg gccgcggaat gccgggcgac
     1861 cctcgccggt tacaaggtgc cgaagcggtt cctcgcgttc gactcgctcc agcgttcgcc
     1921 cgcggggaag gccgactaca acctcctgcg gtcgctcgcg acagaagcct tgggatgatc
     1981 ttcgagcagt actacctgga ttgcctctcg cacgcctcgt atctcatcgc cgatgcatcg
     2041 acggggcgcg cggtcgtcgt cgacccgcag cgtgacgtca cgcagtacat cgaggacgcg
     2101 gcggcccaca actgcacgat cgagcgcgtg atcgaaacgc acttccacgc cgacttcctc
     2161 tccgggcatc tcgagctggc cgcccagacc ggcgccgtca tctcgtacgg cgaggccgcg
     2221 ggtgaaacgt cgttcccgat cgaacgcctc gccgacgggg agcgactctc gctcggcgac
     2281 gtcgtgctcg agatccgggc gacacccggc cacacacccg agtcgatcag cgtggtcgtg
     2341 tacgagcacg ccggcgacgc cgagccgtac ggcgtgctga ccggcgacac gttgttcatc
     2401 ggcgacgtcg gccggcccga tctgctcgcc tcgcgcggca tgaccgccac cgagctcgca
     2461 cgcgacctct tccgctcgac ccgcacacaa ctgctcacgc tccccgacgc gacgcgcgtc
     2521 tacccggcac acggcgcggg gtcggcgtgc ggccggaagc tgtcgaccgc caccgtgtcg
     2581 acgatcggcg agcagcgggc gacgaactac gcacttgccg acatgaccga ggacgcgttc
     2641 gtcgaggtcg tcaccgaagg cctgcccgac gcgcccgcgt acttcgcgtt cgacgcacag
     2701 cgcaatcgtg accaacgcga gttgctcgac gagacgaccg cgccgccgcg cctgtcactg
     2761 gccgacgtgc tcgaccgcgt cgccgcgggc gcagtcgtgc tcgacacgcg cgacgccgcg
     2821 gactacgcgc acgggcactt gcgcgactcg ctcgaagtgg ggctcggcgg ccgcttcgcc
     2881 gaatacgcgg gcgacgtcct ggatcccgac cagccgatca tcctcgtgtg cgacgcgggc
     2941 accgagatcg aggcgaaggt gcggctcgcg cgcatcgggt tcgacaacgt cgtcggtgtg
     3001 ctcgacgacc acgtcaacgc gttcctcgac cggcccgatc tcgtcgaccg gtcctcacgg
     3061 ctcgccgcga ccgagctcgc ggccctgctc gaggcgggcg agaccatcca gctggtcgac
     3121 gtccgcaccg ccgacgagat caccgacggc gcggtgacgg gcgcggtgcg cctgccgctc
     3181 gcccgcatca cgtctg
//
LOCUS       DASUGA010000038         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1100476, whole genome shotgun sequence.
ACCESSION   DASUGA010000038 DASUGA010000000
VERSION     DASUGA010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100476"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..393)
                     /locus_tag="VFX21_00725"
     CDS             complement(109..393)
                     /locus_tag="VFX21_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094497.1"
                     /translation="MTKLVRSEDYESLVLAWLYGQVQSLRAALLEAGIGDEEAVQTVC
                     ETYLFRLAAELDSGTTNIAFERGDTLVLPESDFELHDYAIGVIEEALDDA"
     gene            complement(390..869)
                     /locus_tag="VFX21_00730"
     CDS             complement(390..869)
                     /locus_tag="VFX21_00730"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX5094498.1"
                     /translation="MPAAWRQDSPYGELTVVVVDGRVREISLPGPDQPDIDIGKPVAV
                     VAKQLDQWFYGKRHAFELHLDLEGVSGFRRTVLETLANEVPWGETVSYGELASMAGSP
                     RAARAAGSAMRNNPLPFVIPCHRVLAAGGKIGGYGGGANAIELKRALLAREGVLIPR"
     gene            complement(869..1852)
                     /locus_tag="VFX21_00735"
     CDS             complement(869..1852)
                     /locus_tag="VFX21_00735"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX5094499.1"
                     /translation="MKNPVRVAVTGAAGQIGYSLVFRIASGDLLGPDQPVILSLLEIT
                     PALGALQGVVMELDDCAFPLLHGIETSDDANKAFDGANVALLVGSRPRTKGMERKELL
                     EVNGAIFTTQGKALAAGAADDIRVLVVGNPANTNCLIAMNNAGSIAKERFTAMTRLDH
                     NRAKAQLAARTGVQVASVTNMTIWGNHSATQYPDVYHAQVNGKPAGELVDQAWVAETF
                     IPTVQQRGAAIIEARGASSAASAANAALDHVRDWTLGTAAGDWVSMAVPSDGSYGVPE
                     GLISGFPCTTSGGKWEIVQGLDIDTFSRERIDASVAELGEERDQVAELGLI"
BASE COUNT          336 a          709 c          613 g          346 t
ORIGIN      
        1 acgtggatcg ccctttgaag gcatgcccac cgctggttac gaggaaggcg aattggctcg
       61 caccggttca aacgctgcgc cgccacacgg tgctcggcgc gttcctcgct acgcgtcgtc
      121 gagcgcttct tcgataacgc cgatcgcgta gtcgtggagt tcgaagtcgc tttccggcaa
      181 caccaacgtg tcaccgcgct cgaacgcgat gttggtcgtc ccgctgtcga gttccgcggc
      241 gagccggaag aggtatgtct cgcacacggt ttgcaccgct tcttcgtcgc cgatgcccgc
      301 ctcgagcagc gccgcccgta gcgactgcac ctggccgtac aaccaagcaa gcacgagtga
      361 ttcgtagtct tcggaacgaa cgagcttggt caccgcggga tcagaacgcc ttctcgcgcg
      421 aggagggcgc gcttgagctc gatggcgttg gcgccaccgc cgtagccgcc gatcttgccg
      481 cccgcggcga ggactcggtg acacgggatc acgaacggca gcggattgtt gcgcatcgcc
      541 gaaccggcag cgcgcgccgc gcgcgggctg ccggccatcg acgcgagttc gccgtagctc
      601 accgtctcgc cccacggcac ttcgttcgcc aacgtttcca gcaccgtgcg ccggaagccg
      661 ctgacacctt ccaaatcgag atgcaactcg aacgcgtgac gcttgccgta gaaccactga
      721 tccaactgct tcgccacgac ggcaacgggc ttgccgatgt cgatgtcggg ctgatcgggc
      781 cccggcaacg agatctcgcg cacgcgcccg tcgaccacca cgacggtcag ttccccgtag
      841 ggactgtcct gccgccaggc ggccggcatc agatcagtcc gagctccgcg acctggtcgc
      901 gctcttcgcc cagttccgcc accgacgcgt cgatccgttc ccgcgagaac gtgtcgatgt
      961 cgagcccttg cacgatctcc cacttgccac ccgacgtcgt acacgggaag cccgagatca
     1021 atccttccgg cacaccgtac gaaccgtcgg acggcaccgc catcgacacc caatcgccgg
     1081 cagccgttcc caacgtccag tcgcgcacgt ggtcgagcgc cgcgttcgcg gcggatgccg
     1141 ccgacgatgc gccgcgcgct tcgatgatcg cggcgccgcg ctgctgcacc gtcgggatga
     1201 acgtttcggc aacccacgcc tggtcgacga gttcacccgc gggcttgccg ttcacctgcg
     1261 cgtgatacac gtcggggtac tgcgtcgccg agtggttgcc ccagatcgtc atgttcgtga
     1321 ccgacgcgac ctgcacaccc gtcctcgcag caagctgcgc cttcgcacgg ttgtggtcga
     1381 gccgcgtcat cgcggtgaac cgttccttcg cgatcgaacc ggcattgttc atggcgatca
     1441 agcagttcgt gttggcgggg ttgccaacca cgaggacacg aatatcgtcg gccgcgcctg
     1501 cggcgagcgc cttgccctgg gtcgtgaaga tcgcgccgtt gacttcgagc agttccttgc
     1561 gctccatgcc cttcgtgcgc ggtcgcgatc cgacgagcaa cgcgacgttg gcaccgtcga
     1621 atgccttgtt cgcgtcgtcg ctcgtttcga tgccgtgcag cagcggaaac gcgcagtcgt
     1681 cgagttccat cacgacgcct tgcagcgcac ccaacgcggg tgtgatctcg agcagcgaca
     1741 agatcaccgg ctggtcggga ccgagcagat cacccgatgc gatccgaaaa acgaggctgt
     1801 aaccgatctg accggccgca ccggtcaccg caacgcgaac aggattcttc acgtctgggg
     1861 acgctagccc tcgacggcga cctcatccga agcgacatcg agcaggatcg cccgggcctc
     1921 ggtcagctcc tcgatacgag ctgcggcacc ggcctgcgcg cccccactgt cggggtgcgc
     1981 gtcccgcaac agccgccgga accg
//
LOCUS       DASUGA010000039         4184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_962170, whole genome shotgun sequence.
ACCESSION   DASUGA010000039 DASUGA010000000
VERSION     DASUGA010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962170"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="VFX21_00740"
     CDS             <1..1116
                     /locus_tag="VFX21_00740"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX5094500.1"
                     /translation="LIAQWGHDKRRYSAMKFFLFTMAGSAFLFVGIVSLGFLHRGDTG
                     TLTFDVATLTDWSSTPGNLATETARLLFLAFAVGFAVKVPLFPLHTWLPDAHTDAPTA
                     GSVILAGVLLKMGTYGFMRYALPMFPKASVDLAPILLTVAVIGITYGAIVAAMQPNLK
                     RIIAFSSVAHLGFVVLGTFALTREGVSGGVFTMLSHGLTTSALFLLVGMLYERRHTYE
                     LASYRGLWKAIPVFGGLFVAAAFASIGLPGFSGFVGEFLSLLGTFLTSRWWAVVATTG
                     IVFAAVYLLWAVQRTVMGEPDGDNVGMRDLNVRELMTVVPLLGLSLFLGFFPSPVLDR
                     VQPTVDRLIAHVEERGDYHPDVPRGEEPAVAEVESDK"
     gene            1113..2630
                     /locus_tag="VFX21_00745"
     CDS             1113..2630
                     /locus_tag="VFX21_00745"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX5094501.1"
                     /translation="MTFLAQAVDTAIDTPSIDWFAIAPEIALFGAALLIVLLRAIYRH
                     EPRIHELTLLLAFTGVVTSGVFTLVQWIFVDDNGPYEAINGMVAVDGFAVFVQTVVLI
                     ATLLALLLSAGYLDRERLEAPEYYALMLCSATGMMVMASANDLIIVFLSLEILSIALY
                     VLAAFDRRRMTSQEAGLKYFILGSLSSAILLYGVALTYGATGSTNLTKIAQYLAQTTL
                     LDSGVLMLGFALLLVGLGFKVAAAPFHMWTPDVYEGAPTPVTAFMAGATKAAAFAAIL
                     RVFLGSFDLYRVDWRPIVGALAVLSLLIGSIVALVQTDVKRMLAYSSVNHAGYILIAV
                     YAASAAGTSAALFYLLAYAVMVFGAFAVVTVIARRGDADHALGQYRGLGSRRPILAAL
                     LTLFLLAQAGVPLTGGFVAKLSVFDAAVDAHQYWMALFGMLMAAVAAFVYLRIVLTMY
                     APADEADDTPLSSRRRLDPGAVMALTLAAAAIIVLGVLPDYALDFAHNATQLLAK"
     gene            2743..4131
                     /locus_tag="VFX21_00750"
     CDS             2743..4131
                     /locus_tag="VFX21_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094502.1"
                     /translation="MRKLWTCLALLAFVLVMAGCKVVVTPDDPQSWGFLNEGANGNGY
                     FVNGPGDPPAGRGSAVLAIDGNGREAIASLQYAGTMLNTLSQLRYSTYQAFSGSPNET
                     LYLGFDVDYDSTDSSTAYQGRLVYVPQMAGTVTPKTWQEWDTLTGGAAWYSTGSGASS
                     FRPIVGDAPQASPPCTQTTFCTWSTVLGHYPNARIRPTTGVLLIRAGGPVTGGFVGAT
                     DNLVLQIGSVLVENDFEPGDGHVAVTAANAAGRGFGFVTETNNGTGSFVSGPSGKDGA
                     GSAKLTIDSTGAVALANGLFAGTSLDDFTFLSYKTYTQNASTHATTLQLDVDYDSTDA
                     TTAFQGRAVFEPSVSGQAAIAADTWQTWNPLTAPSGWWQTGNAIVAGGNVGQACTQAV
                     PCSFAQLLAAYPNASVRPVTGQSQGQPIGGGMYLKAGSGWSGGYTGNVDSLTVAVNIG
                     GTNGTVTYDLEP"
BASE COUNT          607 a         1426 c         1373 g          778 t
ORIGIN      
        1 ctcatcgcgc aatggggaca cgacaagcgc cgctactcgg ccatgaagtt cttcctgttc
       61 acgatggcgg ggtccgcatt cctgttcgtc ggtatcgtga gcctcgggtt cttgcaccgc
      121 ggtgacacgg gcacgttgac gttcgacgtc gcgaccctca ccgactggtc gtcgacgccc
      181 ggcaacctcg ccaccgagac cgcccggctg ctgttcttgg cgttcgcggt gggcttcgca
      241 gtcaaggtgc cactgttccc gctccacacc tggctgcccg atgcgcacac cgacgcgccg
      301 actgcgggct ccgtcatcct ggccggcgtc ctgctgaaga tgggcacgta cgggttcatg
      361 cggtacgcgc tgcccatgtt cccgaaggca agtgtcgacc tcgcgccgat cttgttgacg
      421 gtcgcggtga tcggcatcac ctacggcgcc atcgtcgccg cgatgcaacc gaacctgaaa
      481 cggatcatcg cgttctcgtc ggtcgcgcac ctcggcttcg tggtgctcgg caccttcgct
      541 ctgacgcgtg agggtgtgtc gggaggcgtg ttcacgatgc tgtcgcacgg cctcacgacg
      601 agcgcgttgt tccttctcgt cggcatgctc tacgagcgtc gtcacacgta cgagctcgcg
      661 agctaccgcg gcttgtggaa agcgatcccg gtgttcggcg ggttgttcgt cgcggccgcg
      721 ttcgcgtcga tcggcctccc gggcttctcg ggattcgtcg gcgagttcct gtcgctgctc
      781 ggcacgttcc tgacgagccg ttggtgggcg gtcgtcgcga cgaccggtat cgtcttcgcg
      841 gcggtctacc tcttgtgggc ggtgcagcga acggtcatgg gcgagcccga cggcgacaac
      901 gtcgggatgc gcgacctcaa cgtgcgcgag ctgatgacgg ttgtgccgct cctcggtttg
      961 tcgctgttcc tcggcttctt tccctcgccc gtcttggatc gtgtgcagcc gacggtcgac
     1021 cgcttgatcg cccacgtcga ggagcgcggc gactaccacc ccgacgtgcc gcggggcgaa
     1081 gaacccgcgg tggccgaagt tgagagtgac aagtgacgtt tctggcgcag gcggtcgaca
     1141 ccgcgatcga cacgccgtcc atcgactggt tcgcgatcgc gccggagatc gcgttgttcg
     1201 gcgcggcgtt gttgatcgtg ttgctccgcg cgatctaccg ccacgaaccg cggatccacg
     1261 agctcacgct gctcctcgcc ttcacgggcg tcgtgacatc gggcgtgttc acgctcgtgc
     1321 agtggatctt cgtcgacgac aacgggccgt acgaagcgat caacggcatg gtggccgtcg
     1381 acggcttcgc ggtcttcgtg cagacggtcg tgctcatcgc gacgctgctc gcgctcttgc
     1441 tctcggccgg ttacctcgat cgcgagcggc tcgaggcacc cgagtactac gcgctgatgc
     1501 tgtgctcggc caccggcatg atggtcatgg cttcggcgaa cgacctgatc atcgtgttcc
     1561 tgtcgctcga gatcctgtcg atcgcgttgt acgtgctggc cgcgttcgac cggcgccgca
     1621 tgacctccca ggaggcgggt ctcaagtact tcatcttggg gtcgctctcc tcggcgatcc
     1681 tcctgtacgg cgtcgcgctc acctacggcg cgaccggcag caccaacctc acgaagatcg
     1741 cgcagtacct cgcgcagacg acgctgctcg actcgggtgt gttgatgctc ggcttcgcgc
     1801 tgctgctcgt cgggctcggc ttcaaggtcg cggccgcgcc gttccacatg tggactcccg
     1861 acgtctacga gggggcaccg acgcccgtta ccgcgttcat ggccggcgcg acgaaggccg
     1921 ccgcgttcgc cgcgatcctg cgcgtcttcc tcggatcgtt cgatctgtac cgcgtcgact
     1981 ggcgcccgat cgtcggcgcg ctcgcggtcc tgtcactgct cattggctca atcgtcgcac
     2041 tcgtgcagac cgacgtgaag cggatgctcg cgtactcgtc ggtcaaccac gcgggttata
     2101 tcctcatcgc ggtctacgcg gcgtcggccg caggcacgag cgccgcgctc ttctacttgc
     2161 tcgcgtacgc ggtgatggtg ttcggggcgt tcgcggtcgt caccgtgatc gcccggcgcg
     2221 gcgacgcgga tcatgcgctc ggccaataca gaggcttggg ttcgcgtcgt ccgatcctcg
     2281 ccgcgttgct gacgttgttt ctgctcgcgc aggcgggcgt gccgttgacc ggcgggttcg
     2341 tggcgaagct gtcggtgttc gacgccgcgg tcgacgcgca ccagtactgg atggcgttgt
     2401 tcggcatgct catggccgcg gtcgccgcgt tcgtctactt gcgcatcgtc ctcacgatgt
     2461 acgcgccggc cgacgaagcc gacgacacgc cgctgtcgtc gcgccgccgc ctcgaccccg
     2521 gtgccgtcat ggcgctcaca ctggcagcag cggcgatcat cgtgctcggc gtgctgcctg
     2581 actacgcgct cgacttcgcg cacaacgcca cacaactcct cgccaagtaa agagttctct
     2641 ttcgtccgag ttgcggaaac gtgctgactc gcactccttg ttcgcgcgtg gaaacggcga
     2701 gtagtccaaa ccccgttcgc ttctcgtgct tcagggggac tcatgcgcaa gctctggacg
     2761 tgccttgctc tgctcgcgtt cgtgctcgtc atggcggggt gcaaggtcgt cgtcacgccc
     2821 gacgacccgc agagttgggg atttctcaac gaaggcgcca acggcaacgg ctacttcgtc
     2881 aacgggccgg gtgacccgcc cgcgggccgc ggcagcgcgg tgctcgcgat cgacggtaac
     2941 ggccgcgaag cgatcgcgtc gttgcagtac gccggcacca tgctgaacac gctgagccaa
     3001 ctgcgctact cgacgtacca ggcgttctcc ggctcgccga acgagacgct gtatctcggc
     3061 ttcgacgtcg actacgactc gaccgacagc tcgaccgcct accagggccg gctcgtctac
     3121 gtgccgcaga tggccggcac ggtcacgccg aagacgtggc aggagtggga cacgctgacc
     3181 ggcggtgccg cgtggtactc gacgggttcg ggcgcgtcgt cgttccggcc catcgtgggt
     3241 gatgcgccgc aggcgagccc cccgtgcacg cagacgacct tctgcacgtg gagcacggtg
     3301 ctcgggcact acccgaacgc gcgcatccgg cccacgacag gggtgctgct catccgcgcc
     3361 ggtggacccg tgaccggcgg cttcgtcggt gcgaccgaca acctcgtcct gcagatcggc
     3421 tcggtgctcg tggagaacga cttcgagccc ggcgacggcc acgttgcggt gaccgcggcc
     3481 aacgcggccg gtcgtgggtt cggcttcgtg accgagacga acaacggcac cggctcgttc
     3541 gtgagcggcc cgagcggcaa ggacggagcc ggaagcgcga agctcacgat cgactccacc
     3601 ggtgcggtcg cgctcgccaa cggtctgttc gcgggcacga gtctcgacga cttcacgttc
     3661 ctctcgtaca agacgtacac gcagaacgcg agcacgcacg cgacgacgtt gcagctcgac
     3721 gtcgactacg acagcacgga cgcgaccacc gcgttccaag gccgcgcggt gttcgagccg
     3781 agcgtctcgg gacaagccgc gatcgcggcc gacacgtggc agacctggaa cccgctcacc
     3841 gcgccgtcgg gctggtggca gacgggcaac gcgatcgtcg ccggcgggaa cgtcggtcaa
     3901 gcgtgcacgc aagccgtccc gtgctcgttc gcgcaactgc tcgccgcgta cccgaacgca
     3961 tcggtgcgac cggtcaccgg ccaaagccaa ggccagccga tcggcggcgg catgtacctg
     4021 aaggccggga gcggctggtc gggcggctac accggcaacg tcgactcgct caccgtcgcc
     4081 gtgaacatcg gtggcaccaa cggcacggtc acctacgact tggagccgta gagatcactc
     4141 gggcgtcgct gaaccaccac gacggtggtc cagcgacgcc cgac
//
LOCUS       DASUGA010000040         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1378492, whole genome shotgun sequence.
ACCESSION   DASUGA010000040 DASUGA010000000
VERSION     DASUGA010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378492"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFX21_00755"
     CDS             <1..192
                     /locus_tag="VFX21_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase"
                     /protein_id="HEX5094503.1"
                     /translation="ERVYREMVDRMYEYGKAVNVAGLFEIDDVIDPADSRRWISTMLT
                     TAGTPTPRAGKKRPNIDTW"
     gene            complement(206..>2414)
                     /locus_tag="VFX21_00760"
     CDS             complement(206..>2414)
                     /locus_tag="VFX21_00760"
                     /inference="COORDINATES: protein motif:HMM:NF025627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HEX5094504.1"
                     /translation="PTATEAEPGEFNLTPAPASRSAAAVAAAADVHAFASRPDLQPPV
                     MTVVKQTAGVSGYVGTTPRGGAQAGSMISDKLGNPYYFAPNVSFSGIFDVNVQTYKGQ
                     PVLTWWQGEAFGGWGNGEHPLFDQTYRQIATARAGNGYQADVHDMVITPDNDALVIIY
                     NTIQRDLTSVGGSANGVLQEAVVQEVDIATGAVEFEWHSTDDVPLSKSNTPLPADPTK
                     AWDYVHANSVALDADGNILVSARHTSSVYKLNRATGALMWTLGKNGDFTPTNFGDNEW
                     FSGQHDARRRANGNLAMFDNASGNGPPVHPYSRGIELTLNETTKTATLAKELRTNPDV
                     LGTSQGSFRPLGGGRHLVGWGAVGLWTEFDSSGNPVLDVRFPSGVQSYRATSIPAWHG
                     RPTRPPDALVQRINSTTTRVQVSWNGATDVAKWVVLAGPDANHVSAVYARSRAGFETL
                     LDARTRATTIVVEARDASDRPLGRVTKIFQGTASQSSSPVLGYWVARSDGSVGSFGNA
                     PTLTAARVPRGDKVVGIAKRTAGGYWMATAKGDVLAIGAPRYGSTVHRHLTAPIVGIS
                     AAPDGRGYWLVGADGGVFAFGSARFFGSTGNMRLNQPIVGIAATTNGRGYWLVAADGG
                     IFTFGSAPYVGGTGSMALVKPIVGMAVAPKGVGYWLVASDGGVFTFGSARFYGSTGNM
                     HIGAPIAGITPTTDGLGYYLVAQNNALYSFGDANYLGTSQASGTARAVGLIPR"
BASE COUNT          384 a          786 c          823 g          421 t
ORIGIN      
        1 gagcgcgtct atcgcgagat ggtcgaccgc atgtatgaat acggcaaggc ggtcaatgtc
       61 gccgggcttt tcgagatcga cgacgtgatc gatccggccg actcgcggcg ctggatttcc
      121 accatgctca cgacggcggg cacgccgacg ccgcgcgcgg gcaagaagcg tccgaacatc
      181 gacacctggt agccggggcg acgagctagc gggggatcaa tcccactgcg cgtgcggtcc
      241 cgctcgcttg cgacgtgccc aggtagttcg cgtcgccgaa cgagtacagc gcgttgtttt
      301 gggcaacgag gtagtagccg aggccgtcgg tcgtcggtgt gatacctgca atcggcgcgc
      361 cgatgtgcat attgccggtc gacccgtaga accgcgccga gccgaacgtg aaaacgccgc
      421 catcggacgc aaccagccag tacccgacgc ccttcggcgc gacggccatg ccgacgatcg
      481 gtttaacgag cgccatgctg ccggtgccgc cgacgtatgg cgcactcccg aacgtgaaga
      541 tgccgccgtc ggccgccacc agccaatagc ccctgccgtt cgtcgtcgcc gcgattccta
      601 cgatcggttg gttgaggcgc atgttccccg tagagccgaa gaaccgcgcc gatccgaacg
      661 cgaacacgcc accgtcggcc ccgaccagcc aataaccgcg gccgtcgggc gcggcggaaa
      721 taccgacgat cggtgccgtc aggtgacggt gcaccgtcga tccgtaacgg ggtgcaccga
      781 tggcgagcac gtcacccttt gccgttgcca tccagtagcc acccgcggtg cgcttggcga
      841 taccgacaac cttgtcgccg cgtggcacgc gagcagccgt cagtgtgggt gcgttcccga
      901 acgagccgac gcttccgtcg ctgcgcgcaa cccagtagcc caagaccggg ctcgaagact
      961 gcgacgccgt gccttggaag atcttcgtga cgcgaccgag cgggcggtcc gatgcatcgc
     1021 gggcttcgac gacgatggtc gtggctcgcg tcctggcatc gagcagcgtc tcgaacccgg
     1081 cgcggctacg cgcgtacacg gcggaaacgt gattcgcgtc cgggccggcg agcacgaccc
     1141 actttgcgac gtcggtggcg ccgttccagc tcacctggac gcgcgtcgtc gtcgaattga
     1201 tccgctggac cagcgcgtcc ggcggccggg tcgggcggcc atgccaggcg ggaatcgatg
     1261 tcgcccggta ggactgcacg ccgctgggga acctcacatc gagtactggg ttcccgctcg
     1321 agtcgaactc ggtccacagg ccgacggcgc cccaaccgac gagatggcgg ccgccaccga
     1381 gcgggcggaa cgaaccttgg ctcgtgccca gcacgtccgg gttcgtgcgc agctccttcg
     1441 cgagggtcgc cgtcttggtc gtctcgttca atgtgagctc gatgccgcgc gagtacgggt
     1501 gcacgggcgg accgttgccg ctcgcgttgt cgaacatcgc gaggttgccg ttcgcgcgac
     1561 ggcgggcgtc gtgttgaccc gagaaccact cgttgtcgcc gaagttggtt ggagtgaagt
     1621 cgccgttctt gccgagcgtc cacatgagcg cgccggtcgc gcggttgagc ttgtagacgc
     1681 tcgaggtgtg acgcgccgac accaggatgt tgccgtcggc gtcgagcgcg accgagttgg
     1741 cgtggacgta gtcccacgct ttcgtcgggt cggccggaag cggagtgttc gacttgctca
     1801 gcggaacgtc atcggtgctg tgccattcga attccaccgc gccggttgcg atgtcgactt
     1861 cttgcacgac tgcttcctgc agcacgccgt tcgcggagcc gccgaccgaa gtgaggtcgc
     1921 gctggatcgt gttgtagatg atgacgagcg cgtcgttgtc gggtgtgatc accatgtcgt
     1981 ggacgtcagc ttggtaaccg ttgccggcgc gcgcagtcgc gatctgccga taggtctggt
     2041 cgaagagcgg gtgttcgcca ttgccccaac cgccgaatgc ttcgccctgc caccacgtga
     2101 gcacgggctg gcctttgtac gtctgcacgt tcacgtcgaa gattccgctg aacgagacgt
     2161 tgggcgcgaa gtagtagggg ttccccaact tgtccgagat catcgatccc gcttgcgccc
     2221 caccgcgcgg cgtcgtgccg acgtagcccg agacacccgc ggtttgcttc acaacggtca
     2281 tgaccggtgg ttgcaggtcc gggcggctgg cgaacgcgtg cacgtcggcc gcggcggcga
     2341 ccgccgcggc gctacgagac gcgggcgccg gtgtgaggtt gaactcgccg ggttcagcct
     2401 cggtcgcggt aggc
//
LOCUS       DASUGA010000041         3366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_545310, whole genome shotgun sequence.
ACCESSION   DASUGA010000041 DASUGA010000000
VERSION     DASUGA010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545310"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VFX21_00765"
     CDS             complement(<1..378)
                     /locus_tag="VFX21_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094505.1"
                     /translation="MKLLVRLLICAVAFGAIAAVGYVIEGVASADDGDDVLGPGLVIV
                     DVAIHHSTFDGLDGLRVRPGTTVRFVVHNRDPINHELIIGPAAVHQAHEHGTERVHPP
                     VPGEVSVAPLDTGVTFYEFEAPGT"
     gene            complement(375..1421)
                     /locus_tag="VFX21_00770"
     CDS             complement(375..1421)
                     /locus_tag="VFX21_00770"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HEX5094506.1"
                     /translation="MFRRIAVPLGVTAAALTIAASPAFACGGLVGENGTIQLLKTTTL
                     AAYHDGVERYVTSFEFNGSGEAVGSIIPLPDVPSNVERGGDWTLQRLAREVAPKAESR
                     ALALEDASATTVGAEVLLETEIDALDITVLKGGADEVGTWALDNGFFLSPDAPEMLDF
                     YANRSEIFMAAKFDASRAAALGQTTGDGTPIMLTIPTDEPWVPLRILGLGLDKSEVVQ
                     ADVFLLTDDTPKMLAGGPGLSIERSEPANTPLLDDLRSDKGMEWVPSSMWFTYLSLDS
                     SAGDLDYDLALSTHDNALPAITDVGVDADEATPVRVDHDLGNFPLFLGVIGACGLFLV
                     LGYRERTRTKSIAA"
     gene            1536..2366
                     /locus_tag="VFX21_00775"
     CDS             1536..2366
                     /locus_tag="VFX21_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5094507.1"
                     /translation="MSEEVIIVDEPSAGVRRITMNRPDKRNSLIHTLRGAILETLRAH
                     DQDPTVKVTIIRGAGPSFSAGYDLGGGNEGLDMPYYTPGGEGAWPRHVTEGWMSIWDL
                     AKPVIAQVHGYCLAGASELATGCDLVYMAEDAQMGYPAVRFGVPDMHFHPWFLGMRKA
                     MEMMLTGDSVSGIEAAELGWANAAYPADELEARVLEIAQRIALVPSELLQLNKRVVHR
                     QMEHMGLRAGIRAGTELCALGTHTRAMRDFVGKARETSLTQALQERDAPFGDYRTTTH
                     "
     gene            complement(2390..2956)
                     /locus_tag="VFX21_00780"
     CDS             complement(2390..2956)
                     /locus_tag="VFX21_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094508.1"
                     /translation="MELGNLASDIVNVALTVAAVMLVLFLMLCALVFVYARKTGRWVR
                     NTVNRQLVNIAPGPGRELVRLRGRIEHSVRGAQAAVQAATDDGADCGDLRLHLSRLEA
                     AAAQLDGQLERIAVAKLRPEMLKAVLAPLRPRVDKIERIATDLAAVAAMSIAGGTEVE
                     LDLLHERVGGERHFVEARTDALQQLRTN"
     gene            complement(3006..>3366)
                     /locus_tag="VFX21_00785"
     CDS             complement(3006..>3366)
                     /locus_tag="VFX21_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEX5094509.1"
                     /translation="VGAALAAVFGLLDFLTIPRGTPAFRTGLTHLGLNVAVVVLFVAN
                     FLWRHGDWADADKVRAAQIALSVGALVVLAVSGWLGGNLSYRYGVRVAREVDQQIGFT
                     AGSAPELATEETPLASE"
BASE COUNT          555 a         1138 c         1172 g          501 t
ORIGIN      
        1 cgtgccgggt gcctcgaact cgtagaacgt cacacccgtg tcgagcggcg cgaccgagac
       61 ctcgccgggc acgggcggat gcacgcgttc ggtgccgtgc tcgtgggcct ggtgcaccgc
      121 cgccggtccg atgatcagct cgtgattgat cgggtcgcgg ttgtgcacga cgaaccgcac
      181 ggtcgtcccc ggtcgtacgc gcaggccgtc gaggccgtcg aaggtgctgt ggtggatggc
      241 gacgtcgacg attacgagtc ccggacccag cacgtcgtca ccgtcgtccg cgctcgccac
      301 gccctcgatg acgtagccga ccgccgcgat cgcaccgaac gcgaccgcac agatgagcaa
      361 ccgaacgagg agtttcacgc cgcgatcgac ttcgtccgcg tgcgctccct gtacccgagc
      421 acgaggaaca ggccgcatgc accgatcaca ccgaggaaca gcgggaagtt cccgagatcg
      481 tgatcgacgc gcacgggcgt ggcctcgtcg gcgtcgacac cgacgtcggt gatcgcgggc
      541 aacgcgttgt cgtgtgtgga gagcgcaagg tcgtagtcga ggtcaccggc cgacgagtcg
      601 aggctcaggt acgtgaacca catcgacgac ggcacccact ccatgccctt gtcggagcgg
      661 agatcgtcga ggagcggcgt gttcgccggt tcgctgcgct cgatcgacag accggggcca
      721 cccgcgagca tcttcggcgt gtcgtcggtg agcaggaaca cgtcggcctg cacgacttcg
      781 ctcttgtcga ggccgagacc gaggatgcgc aacggcaccc acggctcgtc ggtcgggatc
      841 gtcagcatga tcggcgtgcc gtcaccggtc gtctgtccga gcgcggccgc gcgtgacgcg
      901 tcgaacttgg cggccatgaa gatctcgctg cgatttgcgt agaagtcgag catctccggc
      961 gcgtcgggcg agaggaagaa gccgttgtcg agcgcccacg taccgacttc gtcggcgccg
     1021 cccttgagga ccgtgatgtc gagcgcgtcg atctcggtct ccagtaacac ttcggcaccc
     1081 actgtcgtcg cggacgcgtc ttccaaagcc agagcgcgcg actccgcctt cggcgcgact
     1141 tctcgcgcga gccgttgcag cgtccagtcg ccgccccgtt cgacgttcga cggcacatcg
     1201 ggcaacggga tgatcgaacc gacggcttcg ccggacccgt tgaactcgaa cgacgtgacg
     1261 tagcgctcga cgccgtcgtg ataggcggcg agcgtggtcg tcttcaggag ctggatcgtg
     1321 ccgttctcac ccaccagtcc accgcacgcg aatgccggcg acgcggcgat cgtgagggcg
     1381 gcggcggtga caccgagcgg gactgcgata cgccggaaca tgcctgacct ccgtggtcgt
     1441 ctcgggcatt cgacggcgcc gcagggcgct ccggttcccg ggaggtcggc cttggctagc
     1501 gtcccggccc agatcaggac ctgaggagca ctggcatgag cgaagaagtg atcatcgtcg
     1561 acgagccgtc agcaggtgtg cgccgcatca cgatgaatcg cccggacaag cgcaacagcc
     1621 tgattcacac gctgcggggt gccatcctcg agacgctgcg ggcgcacgac caggacccca
     1681 cagtgaaggt gacgatcatc cgcggcgcag ggccgtcgtt ctccgcgggc tacgacctcg
     1741 gcggcggcaa cgaaggcctc gacatgccgt actacacgcc gggcggcgag ggcgcgtggc
     1801 cgcgccacgt caccgagggg tggatgagca tctgggatct cgcgaagccc gtgatcgcgc
     1861 aggtgcacgg ctactgcctc gccggtgcga gcgagctcgc gaccggttgc gacctcgtgt
     1921 acatggccga ggacgcgcag atggggtacc ccgcggtgcg cttcggtgtc cccgacatgc
     1981 acttccaccc gtggttcctc gggatgcgca aagcgatgga gatgatgctc accggcgact
     2041 cggtgtcggg catcgaagcg gccgagctcg gctgggcgaa cgccgcgtac cccgccgacg
     2101 aactggaagc gcgcgtgctg gagatcgcgc agcggatcgc gttggtgccg agcgagctgc
     2161 tccagctgaa caagcgcgtc gtgcaccgcc agatggagca catggggctg cgggccggca
     2221 tccgcgccgg caccgagctg tgcgcgctcg gtacgcacac gcgggcgatg cgcgacttcg
     2281 tcggcaaggc ccgcgagacg agcctgacgc aggcgttgca ggagcgtgac gccccgttcg
     2341 gcgattaccg cacgacaacg cactgagcgg cgggttgcgc gcctcgaagt cagttggtgc
     2401 ggagctgctg cagcgcgtcg gtgcgggcct cgacgaagtg acgctcgccg ccgacgcgtt
     2461 cgtggagcag gtcgagctcg acctcggtgc cgcccgcgat cgacatcgcg gcgaccgccg
     2521 cgaggtcggt cgcgatgcgc tcgatcttgt cgacacgcgg ccgcagcggc gccaggaccg
     2581 ccttcagcat ctcgggccgg agcttggcga ccgcgatgcg ctcgagctga ccgtcgagct
     2641 gcgcggccgc cgcttcgagc cgcgagagat gcagtcgcag atcgccgcag tcggcgccgt
     2701 cgtcggtggc cgcctgcacc gcggcctgcg caccgcgaac gctgtgctcg atccggcccc
     2761 gcaggcgcac gagctcgcgg ccgggaccgg gtgcgatgtt cacgagttgg cgattgacgg
     2821 tgttgcgtac ccaccgcccg gtcttgcgcg cgtacacgaa cacgagcgcg cacagcatga
     2881 ggaacaggac gagcatcacg gccgcgaccg tcagcgccac gttcacgatg tccgacgcca
     2941 ggtttcccag ctccacggca gacctcctcg ggcgaccgct cgcgaccgta gtgcgtgtct
     3001 gtgcgctatt cggacgcgag cggtgtctcc tcggttgcga gctccggtgc gctcccagcg
     3061 gtgaaaccga tctgctggtc gacttcccgg gcgacgcgaa caccgtaccg gtagctcagg
     3121 ttcccgccca gccatccgga gaccgcgagc acgacgagcg cgccaacgga gagagcgatc
     3181 tgcgcggccc gcaccttgtc cgcgtccgcc cagtcgccgt ggcgccacag gaagttcgcg
     3241 acgaagagca cgacgacggc gacgttgagc ccaaggtgcg tgaggcccgt acgaaacgcc
     3301 ggagtaccgc gagggatcgt caagaagtcg agcagcccga acaccgcagc cagcgctgcg
     3361 ccgaca
//
LOCUS       DASUGA010000042         3120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_822309, whole genome shotgun sequence.
ACCESSION   DASUGA010000042 DASUGA010000000
VERSION     DASUGA010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822309"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..495)
                     /locus_tag="VFX21_00790"
     CDS             complement(16..495)
                     /locus_tag="VFX21_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094510.1"
                     /translation="MARYRRFDLVTTVTEPDALEGVRLRRAGLRAAMTGVEQALAAAV
                     PGREDEWVEGVRDALGVLQEVWTRHVVETESPGAFLDELVEHDPRLANGARRLRAEHG
                     SVLSELLAVDELFAAPSVDTDAVRTRLLSLLCDLAKHRQRGADLVYQAYDVDIGGGS"
     gene            complement(510..1493)
                     /locus_tag="VFX21_00795"
     CDS             complement(510..1493)
                     /locus_tag="VFX21_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5094511.1"
                     /translation="MVMLTMRQDFRAPAFGPTSTRDLYAAAMEQFRWADDNGFDFLVL
                     SEHHGVDDGWMPAPLTMAAMVLGATKRAPVLVSAALVPLHDPVRLAEQLAVIDNAFPG
                     RLWTVLGAGYREAEFAMADVDHKGRGKLLEEYVDVMLRAWSGEPVEWRGRTIVVTPTP
                     PNGRPTVLVGGGVAAAARRAARLRLPMLPMNTDQAIIDAYNDEAAKVGFEGGFVMSPQ
                     GPTFIHVSDDPERAWEEIAPYVLYETQTYASFQTPGQHSTPRVNASNVEELKQAEQIW
                     VGTPDDIAARAADLPPIAALNFNPMAGGLPPDLAWASLELFRVKVLPKLRP"
     gene            complement(1650..2618)
                     /locus_tag="VFX21_00800"
     CDS             complement(1650..2618)
                     /locus_tag="VFX21_00800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014054284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX5094512.1"
                     /translation="MKTVSIVPDGTLAYGIQLPVQALSVRTSAPWEREHSTIDDMVRV
                     AQACDDAGFLYVAVCHHVAVPREPAEMMSTQWFDPVPTLAYLAAQTTRTRLMTNVYVV
                     AYNHPLQTAKAFATLDALSGGRVILGVGAGHVEGEFDALGVPFNDRGRITDEAIDAIA
                     NALEHEWPDHAGDRWKFADVGQRPRPVQTPRPPIWVGGSGKPALRRVAARGDGWIPQG
                     TPRTQLPDDIAYILRHRDDVRPGAVPEVGYITEYVYVGEPGWEIPKYSVSGSPQKLVD
                     NFNTLGEMGVSHLQLRFASRSAPELCDQIAAFGADVGRHLVRRALT"
     gene            complement(2630..>3120)
                     /locus_tag="VFX21_00805"
     CDS             complement(2630..>3120)
                     /locus_tag="VFX21_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003990997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5094513.1"
                     /translation="YEPGVVVGPVITAAHRDRVEGYVQIGRDEGGEVVAGGTRPEHPD
                     KGFYVAPTLIAGCNAGMRVAQEEIFGPVIVAIPFDDDDEGIAIANSTDFGLYDYVFSA
                     DTNRAFRASKRLRAGNVGINTTQRNHNAPFGGMKYSGIGRDGGVFGLHAYSELQSVVW
                     PG"
BASE COUNT          453 a         1092 c         1095 g          480 t
ORIGIN      
        1 ccccccacgc ccgggtcagc tgccgccgcc gatgtcgacg tcgtaggcct gatagacgag
       61 gtcggcgcca cgctggcggt gcttggccag gtcgcacagc aggctgagca gccgggtgcg
      121 gaccgcgtcg gtgtcgacgg aaggcgccgc gaagagctca tcgaccgcga gcagctccga
      181 cagcaccgat ccgtgctcgg cgcggaggcg ccgggcaccg ttggcaaggc gcgggtcgtg
      241 ctcgaccaac tcgtcgagga acgcaccggg cgactcggtc tcgacgacgt ggcgcgtcca
      301 gacctcttgg agcacaccga gcgcgtcgcg cacgccttcg acccattcgt cttcgcggcc
      361 gggcaccgcg gcggccagcg cctgctcgac gccggtcatt gccgcgcgca atccggcgcg
      421 gcgcagacgg acgccttcga gggcgtcggg ctccgtgacg gtcgtgacca gatcgaaccg
      481 gcggtagcgg gccatgcccg agtatcgacc tacggtcgga gctttggcaa caccttcacg
      541 cggaacagtt cgagactggc ccacgcgaga tcgggcggca gcccgcccgc catgggattg
      601 aagttcaatg ccgcgatcgg tggcaggtcg gccgcgcgcg ctgcgatgtc gtcgggcgtg
      661 ccgacccaga tctgttcagc ttgcttcagc tcctcgacgt tcgacgcgtt cacgcgcggc
      721 gtcgagtgct gacccggcgt ctggaaggac gcgtacgtct gcgtctcgta cagcacgtac
      781 ggcgcgatct cttcccacgc gcgttcgggg tcgtcggaca cgtggatgaa cgtcgggccc
      841 tgcgggctca tcacgaagcc accctcgaag ccgaccttgg ccgcctcgtc gttgtacgcg
      901 tcgatgatcg cctggtcggt gttcatcggc agcatcggca accgcagccg cgccgcgcgc
      961 cgcgccgcgg ccgccacgcc cccgccgacg agcacggtcg ggcgaccgtt cggcggcgtc
     1021 ggcgtgacga cgatcgtgcg gccgcgccat tcgaccggct cacccgacca cgcgcgcaac
     1081 atgacgtcga cgtattcctc gagcagcttg ccgcggccct tgtggtcgac gtcggccatc
     1141 gcgaactctg cctcgcggta gcccgcgccg agcacggtcc agagccggcc cgggaacgcg
     1201 ttgtcgatga ccgcgagctg ttcggcgagg cggaccgggt cgtgcagcgg cacgagcgcg
     1261 gcggacacga ggaccggtgc gcgtttcgtc gcgccgagca ccatcgccgc catcgtcaac
     1321 ggcgcgggca tccagccgtc gtcgacgccg tggtgctccg agagcacgag gaagtcgaag
     1381 ccgttgtcgt cggcccagcg gaactgctcc atcgcggccg catagaggtc gcgcgtcgat
     1441 gtcgggccga aggccggcgc ccggaagtcc tggcgcatcg tcaacatgac catgagagct
     1501 ccttcagggc tgcgcccaca gggcgaggac ggcaacgcct ccaccttgct gttccgaccg
     1561 acgaagcctc gcaagctcag ccgtcggtcg gagcgtgtgc ggatggacgt gaggcggctg
     1621 cgccgcgtgg ggccaatccg cgcccgctct catgtgagcg cgcggcggac gaggtggcgg
     1681 ccgacgtccg cgccgaacgc ggcgatctgg tcgcacagct ccggcgcgga gcggctcgcg
     1741 aaccgcagtt gcagatggct gacacccatc tcgcccaggg tgttgaagtt gtcgacgagc
     1801 ttctgtggcg accccgacac cgagtacttc gggatctccc atccgggttc gccgacgtag
     1861 acgtattccg tgatgtagcc gacctcgggc accgcgccgg gccgcacgtc gtcgcggtgg
     1921 cgcaggatgt acgcgatgtc gtcgggcagt tgcgtgcggg gcgtgccttg cgggatccag
     1981 ccgtcgccgc gcgcggcgac gcgccgcagc gcgggcttgc ccgaaccgcc gacccagatc
     2041 ggcgggcgcg gcgtctgcac cggacgcggg cgctggccga cgtcggcgaa cttccagcgg
     2101 tcaccggcgt gatcgggcca ctcgtgttcg agcgcgttgg cgatcgcgtc gatcgcttcg
     2161 tcggtgatgc ggccacggtc gttgaacggc acgcccaacg cgtcgaattc gccttcgacg
     2221 tgcccggcac cgacaccgag gatcacgcga ccccccgaca aggcatcgag cgtcgcgaac
     2281 gccttcgcgg tttgcagcgg atggttgtac gcgaccacgt acacgttcgt catgagccgc
     2341 gtgcgcgtgg tctgcgcggc gagatacgca agggtcggca cgggatcgaa ccactgcgtg
     2401 ctcatcatct cggccggctc gcgcggcacc gcgacgtggt ggcacaccgc gacgtacagg
     2461 aatcccgcgt cgtcgcacgc ctgggcgacg cggaccatgt cgtcgatcgt cgagtgctcg
     2521 cgctcccacg gcgccgacgt gcgcaccgag agcgcctgca ccggcagctg gatgccgtag
     2581 gccagcgtgc cgtcagggac gatcgagacc gtcttcatgc cggctgggct caaccgggcc
     2641 acacgacgga ttgcagctcg ctgtacgcgt ggagcccgaa gacaccgccg tcgcggccga
     2701 taccgctgta cttcatgccg ccgaacggcg cgttgtggtt gcgctgcgtc gtgttgatcc
     2761 cgacgttgcc cgcgcgcaac cgcttgctcg cccggaacgc gcggttcgtg tccgcgctga
     2821 acacgtagtc gtacaggccg aagtcggtgc tgttcgcgat cgcgatgcct tcgtcgtcgt
     2881 cgtcgaacgg gatcgcgacg atgacggggc cgaagatctc ttcctgcgcg acgcgcatgc
     2941 cggcgttgca gcccgcgatg agcgtcggcg cgacgtagaa ccccttgtcg ggatgctcgg
     3001 gtcgcgtgcc gccggcgacg acctcgccgc cttcgtcgcg cccgatctgc acgtagcctt
     3061 cgacgcggtc gcggtgcgcg gcggtgatga ccggaccgac gaccacaccc ggctcgtacg
//
LOCUS       DASUGA010000043         4405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_545282, whole genome shotgun sequence.
ACCESSION   DASUGA010000043 DASUGA010000000
VERSION     DASUGA010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545282"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1740
                     /locus_tag="VFX21_00810"
     CDS             <1..1740
                     /locus_tag="VFX21_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5094514.1"
                     /translation="FPGETHQFAPALVHDNHFVDAPRSVADGYHLTEDLVDHATAFIR
                     DLRHVDADKPFFLYFTPGACHSPHQSPPEWLARYRGRFDEGWDVWRDRAFARQLEMGL
                     LPAHTKMSERPDWVPPWDSLSADEKKVYARFMEAFAAYLSHADHHIGRLLDNLRATGD
                     LDRTLIMLCSDNGASSEGGLQGSINDNRLWNFAPRSVEEALEVIDDIGGPRIHNNYPW
                     GWTVAGNTPFRRWKREVHEGGVADPLIVHWPQGITARGEIRHQYVHVVDIVPTVMDAL
                     GFDPPVEFDGVSFAHTFAHADAATHHNVQYYEMLGCRALYFEGWKAVVYHPIQFDQPG
                     LDLAEWELYDVEADPAETTDLAAEHPERLQAMVDLWWKEAERNNVLPLDNRAFADFVF
                     ERPPSAPDRDTYVYWPHTGMVSEESGVNVRNRDHTVTAHIDGPGEGVLISQGSKLGGW
                     TLFVKDGVLSYVHNLASWRVYRVDAPVEIGPGAHTIGFRFTKTGDSTGTGALVVDDNV
                     VGEAPIKRFTPVRFSLTGVGLWCGRGGNLAVADDYEGPFPWTGALDRVVVDVTGPPYV
                     DAEAEADIILKTE"
     gene            1771..2952
                     /locus_tag="VFX21_00815"
     CDS             1771..2952
                     /locus_tag="VFX21_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5094515.1"
                     /translation="MDLSATPEEAVLRDEVRAWLRANLPWEYGKGLPPRFDDLAEEVA
                     FGRAWQAKLAGGRWVGVAWPEEYGGRGGGPVEHYLVTEELARARAPELVGRIGVNLVG
                     PTLLAHGTDAQRARWLPNILNASELWCQLFSEPAAGSDLTSLRTRAEKVDGGFRVNGQ
                     KVWTSYAQFADWGWCLARTNPDAPKNKGISALVVDMHAPGVEVRPLRQITDETEFNEV
                     FFDDVFVPDDRLVGPLDEGWRVANSTLTHERGVNPRQLVIHSQLLDELWRLGLDRGAF
                     ADPRLAPRLAQAFVEVRIFQLHNWRSISRTAKGEDPGPVGSINKLWWSEMSKRLHDTA
                     MAVVGEAAPLWRGAAHNPGAGAWQRSWLYYQASSIWAGTNEIQRNVIGERTLGLPREP
                     R"
     gene            3024..3434
                     /locus_tag="VFX21_00820"
     CDS             3024..3434
                     /locus_tag="VFX21_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX5094516.1"
                     /translation="MRTSHRNSRRLLALVAAGLLVLGLSACGGDDDDDNTAATTETTG
                     GEATTGGGGGGGLNVTIKAFAFTDDASPTAGGEIKVENQDGVTHTFTSDDGEWESIEI
                     SGGDTGTTTAPSAPGEYKFHCEIHKSMTGTLTVE"
     gene            complement(3470..3964)
                     /locus_tag="VFX21_00825"
     CDS             complement(3470..3964)
                     /locus_tag="VFX21_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094517.1"
                     /translation="MNPPVPEPVPAAVAVGMLLAPVVACLRGDDEGVGVLLDGACEDG
                     VAAGALRAAPAVVRVYLRLAPPPDGADRIVASYADGARRRFDRDAFTVGAECLEVARV
                     PAAIERIADAVFADIAGVHGDRCALEGAVASCWWCAQRSAELRNVDPVEEAAAICRYL
                     SRVA"
     gene            4034..>4405
                     /locus_tag="VFX21_00830"
     CDS             4034..>4405
                     /locus_tag="VFX21_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5094518.1"
                     /translation="MGISTLRYEVSGPVATITLARPDAANAQNSQMIDELDAAFDRAD
                     ADDDVRVVVLAGEGKHFSAGHDLKELLAGDEHWAAMRDTPEGKLRHEQVMYFDKLVKI
                     RDFRKPTIAAVQGACSAAGLML"
BASE COUNT          745 a         1511 c         1510 g          639 t
ORIGIN      
        1 ttcccgggtg agacgcatca gttcgcgccc gcgctcgtgc acgacaacca cttcgtcgac
       61 gcgccccgct cggtcgccga cggctaccac ctcaccgaag acctggtcga tcacgcgacg
      121 gcgttcatcc gcgacctgcg ccacgtcgac gcggacaagc cgttcttctt gtacttcacg
      181 ccgggcgcat gccactcgcc gcaccagtcg ccgccggagt ggctggcgcg ctaccgcggc
      241 cgcttcgacg agggctggga cgtctggcgc gaccgcgcgt tcgcccgcca gctcgagatg
      301 ggcctgctgc ccgcgcacac gaagatgtcg gagcgccccg actgggtgcc accctgggac
      361 tcgctctcgg ccgacgagaa gaaggtgtac gcgcgcttca tggaggcgtt cgccgcgtac
      421 ctgtcacatg ccgaccatca catcgggcgg ctgctcgaca atctgcgggc gacgggcgac
      481 ctcgaccgga cgctgatcat gttgtgctcc gacaacggtg cgagcagcga aggcggcttg
      541 caagggtcga tcaacgacaa ccggttgtgg aacttcgcgc cgcgctccgt cgaggaagca
      601 ctcgaagtga tcgacgacat cggcggcccg cgcatccaca acaactatcc gtggggctgg
      661 acggtcgcgg ggaacacgcc gttccggcgt tggaagcgtg aggtgcacga aggtggcgtc
      721 gcggacccgt tgatcgtgca ctggccgcag ggcatcacgg ccaggggcga gatccgtcac
      781 cagtacgtgc acgtcgtcga catcgtgcca accgtgatgg acgcgctcgg gttcgatccg
      841 cccgtcgagt tcgacggcgt cagcttcgcg cacacgttcg cgcacgcgga cgcggcgacg
      901 catcacaacg tgcagtacta cgagatgctc ggctgccgcg cgctgtactt cgagggctgg
      961 aaggcggtcg tgtaccaccc gatccagttc gaccagcccg gcctcgacct cgcggagtgg
     1021 gagctgtacg acgtcgaagc cgatccggcc gagacgaccg atcttgccgc ggagcatccc
     1081 gaacgactgc aagccatggt cgacctgtgg tggaaggaag cggagcgcaa caacgtgttg
     1141 cccctcgaca accgtgcgtt cgcagacttc gtgttcgagc ggccgccgtc ggcgcccgat
     1201 cgcgacacat acgtgtactg gccgcacacc ggcatggtct ccgaggagtc gggcgtcaac
     1261 gtgcgcaacc gcgaccacac cgtgaccgcg cacatcgacg gtcccggcga aggtgtcctg
     1321 atctcgcaag gttcgaagct cggtgggtgg acgctgttcg tcaaggacgg cgtcctgtcg
     1381 tatgtgcaca acctcgcgag ctggcgcgtg taccgcgtcg acgcgccggt cgagatcggt
     1441 cccggcgcgc acacgatcgg gttccggttc acgaagaccg gcgactcgac cggcaccgga
     1501 gcgctggtgg tcgacgacaa cgtggtcggt gaggcgccga tcaaacggtt cacaccagtg
     1561 cgcttctccc tcacgggtgt cggtttgtgg tgcggtcggg gcggcaacct cgccgtcgcc
     1621 gacgactacg aaggcccgtt cccgtggacc ggcgcgctcg accgggtcgt cgtcgacgtc
     1681 acggggccgc cgtacgtcga cgccgaggcc gaggccgaca tcattctgaa gaccgagtag
     1741 ccgcgccaac tcacgtaacc tccgcgaccg atggatctgt cggccacacc ggaagaagcg
     1801 gtgctgcgcg acgaggtgcg tgcgtggctg cgtgcgaacc tgccgtggga atacggcaag
     1861 ggactaccgc cccgcttcga cgacctcgcc gaggaagtgg ccttcgggcg ggcgtggcag
     1921 gccaagctcg cgggcgggcg ttgggtcggt gtcgcgtggc ccgaggagta cggcggccgc
     1981 ggtggcggtc cggtcgagca ctacctcgtc accgaggagc tggcgcgggc ccgcgcaccc
     2041 gaactggtcg gtcggatcgg cgtcaacctc gtcggtccga cgctgctcgc gcacggcacc
     2101 gacgcgcagc gcgctcggtg gctgccgaac atcctgaacg cgagcgagct gtggtgccag
     2161 ctgttcagtg agcccgcggc cgggagcgac ctgacgtcac tgcggacgcg cgcggagaag
     2221 gtcgacggcg gctttcgcgt caacggccag aaggtgtgga cgagctacgc gcagttcgcg
     2281 gattgggggt ggtgcctcgc tcgcacgaac ccggatgcgc cgaagaacaa gggcatctcg
     2341 gcgctggtcg tcgacatgca cgcgcccggc gtcgaggtgc gaccgctccg gcagatcacc
     2401 gacgagaccg agttcaacga ggtcttcttc gacgacgtgt tcgtccccga cgaccggctc
     2461 gtcggaccgc tcgacgaggg ttggcgggtc gccaactcga cgttgacgca cgaacggggc
     2521 gtgaacccgc gccagctcgt catccattcg cagctcctcg acgagttgtg gcggctcggc
     2581 ctcgatcgcg gcgcgttcgc cgacccgcgt ctggcgccgc ggctcgcgca ggccttcgtc
     2641 gaagtgcgca tcttccagct gcacaactgg cgctcgatct cgcgcacggc caagggcgaa
     2701 gatcccgggc cggtcggcag catcaacaag ttgtggtgga gtgagatgag caagcggctg
     2761 cacgacaccg cgatggcggt cgtcggcgag gccgcgccgc tgtggcgggg cgcggcgcac
     2821 aaccccggcg ccggcgcgtg gcaacggtcg tggctctact accaggccag ctcgatctgg
     2881 gccggcacca acgagatcca gcgcaacgtg attggagagc gcacgctcgg actgccgcgc
     2941 gagccccggt agcgagcgaa aaatttcggc ttcctgtgaa ccggtccgcc gctcggctcc
     3001 gtatgacagg catgatgcgc gacatgcgaa cctctcacag gaacagccgc cgccttctcg
     3061 ctctcgttgc cgccgggttg ctcgtgctcg gtctcagcgc gtgcggcggt gacgacgatg
     3121 acgacaacac cgccgccacg accgagacga ccggcggcga ggcgacaacc ggtggcggtg
     3181 gtggtggcgg cttgaacgtg acgatcaaag cgttcgcgtt cacggacgac gcgtcaccga
     3241 ccgccggcgg cgagatcaag gtcgagaacc aggacggcgt cacccacacc ttcacatccg
     3301 acgacggcga gtgggagtcg atcgagatca gcggtggcga caccggcacc acgaccgcgc
     3361 ccagcgcgcc gggcgagtac aagttccact gcgagatcca caaatcgatg acgggcacac
     3421 tcaccgtcga ataagcggcc cgcagaagat cctcaaccgc acggatgcgc tacgcgaccc
     3481 gggagaggta gcggcagatc gctgccgctt cctcgaccgg gtcgacgttt ctcagctccg
     3541 cgctgcgctg cgcgcaccac cagcacgagg cgactgcgcc ttcgagcgcg caccggtcgc
     3601 cgtgcacacc ggcgatatcc gcgaagaccg cgtccgcgat gcgttcgatc gcggccggta
     3661 cccgggcgac ctcgaggcac tcggccccga ccgtgaacgc atcgcggtcg aaccggcggc
     3721 gggccccgtc ggcgtaggac gcgacgatcc ggtcggcgcc atcgggtggt ggtgcgaggc
     3781 gcaagtacac gcggacgacc gcgggtgccg cgcgcagcgc acccgcggcg acaccgtctt
     3841 cgcatgcgcc gtcgagcagg acgccgacac cctcgtcgtc gccgcgcaag cacgcgacga
     3901 ccggggccag cagcatccct accgcgaccg cggcgggcac gggctccgga accggcgggt
     3961 tcatcgcacc ctcagtatcg gcacaccgta cccacgcagt aagtgcggcc cgcggcacta
     4021 cgatccgccg ctgatgggca tttccacgct ccgctacgaa gtcagcggcc cggtcgcgac
     4081 gatcacgctg gcacgccccg acgccgccaa cgcgcagaac agccagatga tcgacgagct
     4141 ggatgccgcg ttcgaccgcg ccgacgccga cgacgatgtg cgcgtcgtcg tgctcgccgg
     4201 agaaggcaag catttctcgg ccggccatga tctgaaggaa ctgctggccg gcgacgagca
     4261 ctgggccgcg atgcgcgaca cgcccgaggg caagctgcgc cacgagcaag tcatgtactt
     4321 cgacaagctc gtgaagatcc gtgacttccg caagccgacg atcgccgcgg tccagggcgc
     4381 gtgctcggcc gcgggactga tgctc
//
LOCUS       DASUGA010000044         2180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_126009, whole genome shotgun sequence.
ACCESSION   DASUGA010000044 DASUGA010000000
VERSION     DASUGA010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126009"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VFX21_00835"
     CDS             <1..531
                     /locus_tag="VFX21_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016825328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5094519.1"
                     /translation="DHIPIATMPGMRERTVTISSAGKTFSMTGWKTGWACAPAPLIEA
                     VRTVKQFMTYVHGAPFQHAAAAGLRLPDEYFTGLAAELATKRDLLAAGLVDAGFTVYP
                     PSGTYFVTADIRAFGETDGMAFCRSLPERCGVVAVPTVVFYDDVEAGAPLVRFTFCKR
                     REVLEEAVVRLKGMAR"
     gene            562..1344
                     /locus_tag="VFX21_00840"
     CDS             562..1344
                     /locus_tag="VFX21_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX5094520.1"
                     /translation="MKVSAVQHDIVWEDPDANFARLAPMIADAAAAGARLVALTELYS
                     TGFSLETARTAEAPGGESEQFVADQAIAQGVWVGASMPVRDGSNLPFNRFVLAGPAGE
                     RYHYDKIHPFSYGHEDEHYAAGTGRVTVDVEGVRVTLFVCYDLRFADQWWAAARDTDC
                     YVVVANWPAGRREHWRTLARARAIENQAYVIAANRVGSGGGLDYAGDSAVYGPFGEEL
                     AIAADTGTIGTEEIVYADVDPEHVAKIRARYPFLQDRRASGD"
     gene            1350..1559
                     /locus_tag="VFX21_00845"
     CDS             1350..1559
                     /locus_tag="VFX21_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094521.1"
                     /translation="MKASTAVTHRVLSAVLTAALSALAFAQGTEGWAIVGAVLLGAAI
                     VDVILLVVVAWFRWRHQDHRADHRA"
     gene            complement(1561..1869)
                     /locus_tag="VFX21_00850"
     CDS             complement(1561..1869)
                     /locus_tag="VFX21_00850"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEX5094522.1"
                     /translation="MQFSPDLRDRVADGTITVSIRLWSRPKVKVGGVYRSGDVTIEVD
                     DIDLLPFSSLTDDDLARTGEPDRESLRRRAAHAGPIDDDTLAFRIEFHVVDEPRETST
                     "
     gene            complement(1976..>2180)
                     /locus_tag="VFX21_00855"
     CDS             complement(1976..>2180)
                     /locus_tag="VFX21_00855"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HEX5094523.1"
                     /translation="GGSNSLFDPTVEGVRLIGRYGANSQHTMYYIVETDDWQALQRFL
                     MPGFKTTAATITPVSEEPLHRTT"
BASE COUNT          309 a          747 c          773 g          351 t
ORIGIN      
        1 gaccacatcc ccatcgcgac gatgccgggg atgcgcgagc gcacggtgac gatctcgtcg
       61 gcgggcaaga cgttctcgat gacggggtgg aagacgggct gggcgtgcgc accggcgccg
      121 ttgatcgagg ccgtgcgcac ggtgaagcag ttcatgacct acgtgcacgg ggcgccgttc
      181 cagcacgcgg ccgccgcggg cttgcggctc cccgacgagt acttcacggg cctcgccgcg
      241 gagctcgcga cgaagcgcga cctgctcgcc gccgggctcg tcgacgccgg gttcaccgtc
      301 tacccgccgt cgggcacgta cttcgtcacc gccgacatcc gcgcgttcgg cgagaccgac
      361 ggcatggcgt tctgccgctc gcttccggag cgctgcgggg tcgtcgcggt cccgaccgtg
      421 gtgttctacg acgacgtcga ggccggtgcg ccgctcgtac ggttcacgtt ctgcaagcgc
      481 cgcgaggtgc tcgaagaggc ggtcgttcgc ctcaagggga tggcgaggtg aagggcctgc
      541 aacgttgaga ggacgggcgt cgtgaaggtt tccgccgttc agcacgacat cgtgtgggaa
      601 gatcccgacg ccaacttcgc gcgcctggcg ccgatgatcg ccgacgccgc ggccgcgggt
      661 gcacggctgg tggcgctcac cgagctgtac tcgaccggct tctcgctcga gacggcccgc
      721 accgccgaag cgccgggtgg cgagagcgag cagttcgtcg ccgaccaggc gatcgcccag
      781 ggtgtctggg tcggcgcgtc gatgccggtg cgcgacggct cgaacctgcc gttcaaccgg
      841 ttcgtgctcg ccgggccggc gggggagcgg taccactacg acaagatcca tccgttctcc
      901 tacgggcacg aggacgagca ctacgcggcc ggcaccgggc gcgtcaccgt cgacgtcgaa
      961 ggagttcgcg tcacgttgtt cgtctgctac gacctgcgct tcgccgacca gtggtgggcc
     1021 gcggcgcgcg acaccgactg ctacgtggtg gtggcgaact ggcccgcggg tcgccgcgag
     1081 cactggcgca ccttggcgcg agcgcgcgcg atcgagaacc aggcctacgt gatcgccgcg
     1141 aaccgcgtcg gatcgggcgg cggtctcgac tacgcgggcg actccgcggt ctacggcccg
     1201 ttcggcgagg agctcgcgat cgccgccgac accggcacga tcggcaccga ggagatcgtg
     1261 tacgccgacg tcgatcccga gcacgtcgcg aagatccgcg cgcggtatcc cttcctccag
     1321 gaccgccgcg cctcaggtga ctgattcccg tgaaggcgtc gaccgcggtc acgcaccggg
     1381 tgctctcggc ggtgctcacc gccgcgctct cggcgctcgc gttcgcgcaa gggaccgaag
     1441 gctgggcgat cgtcggagca gtgctgctcg gtgccgcgat cgtcgacgtg atcctcctcg
     1501 tcgtggttgc gtggttccgc tggcgtcacc aggaccatcg cgccgaccat cgcgcctgac
     1561 tcacgtgctc gtttcacgcg gctcgtcgac gacgtggaac tcgatccgga acgcgagcgt
     1621 gtcatcgtcg atgggtccgg catgcgcggc gcgccgccgc agactctcgc ggtcgggctc
     1681 accagtccgc gcgaggtcgt cgtcggtcag ggacgagaac ggcagcaggt cgatgtcgtc
     1741 gacctcgatc gtcacgtcgc cgctgcggta gacgccgccc accttcactt tcggtcgcga
     1801 ccagagccgg atcgacaccg tgatcgtccc gtccgcgacg cggtcgcgca gatcagggct
     1861 gaactgcatc cgcccagtgt tcccccagcc gcactacagg atgtcggcgg cgtgcccggc
     1921 gcgcatgcgc ccacggtgtc acagctcgtc tgattggcag tgatgacgcg cgccgtcagg
     1981 tggtgcggtg gagcggctcc tccgacaccg gcgtgatcgt tgccgcggtg gtcttgaacc
     2041 ccggcatgag gaagcgctgg agcgcctgcc agtcgtcggt ttcgacgatg tagtacatgg
     2101 tgtgctgcga gttcgcgccg tagcggccga tcagccgcac gccctcgacg gtcggatcga
     2161 acagcgagtt cgaaccgccg
//
LOCUS       DASUGA010000045         8263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1515074, whole genome shotgun sequence.
ACCESSION   DASUGA010000045 DASUGA010000000
VERSION     DASUGA010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8263
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515074"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VFX21_00860"
     CDS             complement(<1..910)
                     /locus_tag="VFX21_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX5094524.1"
                     /translation="MQDHVTTTQTLDVPVQGMTCASCAARIEQSLAKLPGVDAVRVNF
                     ATGRAAVDYEPGHLDPTSVATAITDLGYVVPEIEAVDPDADEARAVLRRLVPAALLTL
                     PLLAVSMIEPLQFAGWRGWALLLATPVVWWAGWPFHRVALAGARHRTTTMDTLISVGT
                     AAAYFWSVAALFVDGAEIYFETAAVITTLLLLGRYFEARARSRAGSALRALLAIGAKT
                     ARLENGDEVPVASLLRGDRFVVRPGEKIATDGVVVGGASAVDVSMLTGEPVPVDVAAG
                     DAVFGATVNTSGRLVVEATRVGEETAL"
     gene            963..1238
                     /locus_tag="VFX21_00865"
     CDS             963..1238
                     /locus_tag="VFX21_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX5094525.1"
                     /translation="MRGYTMHKDDYLNRLRRIEGQVRGLQRMVNEDEYCIDVLTQIAS
                     VSKALQGVGLGLLDEHLRHCVTEAVHSGEGDAKIQEAVQAVERLLRA"
     gene            1375..4563
                     /locus_tag="VFX21_00870"
     CDS             1375..4563
                     /locus_tag="VFX21_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX5094526.1"
                     /translation="MRSQGTRRAIWVLPALLLVAGAALARGPSAPAPAAVDLAVRGSV
                     EQISVTHATPGAPLQLRDAGNDVVGEATVDDLGSVLWRNVAPGDGYEVVELDGTTTGP
                     VHVLAQDELPAQSLYANQELHDGFQYITTRDGTKLSVSVKLPGPAANGPYPTVVEYSG
                     YAASQPNTPQPSTQIAQLLGYATVAVNVRGTGCSGGAFSYFEPLQGLDGYDVIETVAA
                     QPWALHGKAGMIGISYPGISQLFVARTQPPHLAAIAPLSVIDDTYRSTLYPGGIFNNG
                     FALSWAKERMDQAAPYGQGWEQGVVDAGGANGQQCAENQLLRHQNQNLLAEISAHPFY
                     DPALGDALSPVTFADKFDTPVFIGGAWQDEQTGGHFTAMWSKFATKAKMFGTNGTHVD
                     SLLAELNRWYEFLEFYVAERTPQVPTLVRQAAPLLVQSQIGIGGYQLEPDRFTGYPTF
                     AEALAAYEAEPPVRILMENGAGDAANPGSPVGTFEMGFASWPPPQTQATTWFFQPDQH
                     LTASAPTVADDDGRASTSYVYDPTAKPATDFSGSSSDIWQATPDYDWKPLPIGKALAF
                     DSDALTAETVMAGTGSVDLWLRSTAADTDLEVTLTEVRPDGQEMYVQSGWLRASHRAL
                     DPAQSTDLRPWHTHAEADAEPLPKGEFVSARVELFPFAHVFHAGSKLRITVEAPGGNR
                     PLWTFDALDADGTVVNDIAHSVGHPSKVVLPVIPGVDAPDALPPCPSLRGQPCRTALG
                     AAQPIGVQADVTGQDATVTWTPPPARPDVTVESYRVTEAPGGATKDVGANGAATPAAT
                     SAAPLSVQFTGLANGTHSFSVEARYAQGGSSGPSTPSNSVTAAAQEESTTTTTATPTT
                     TTTVAPLNSADATTSTTESDDELDDDFADDESGDFSDDTSDDFSDDFSDDFSTDPCAP
                     TDDFSDPTFDDPTTDTTAVDDFDDFDDFGTEDLSPDPSELSDDPDFMLHGTQETDELT
                     PEDLGLTQEEFDQLYGTGDTTDTTDPFSSTDDFSTTDDFSSDPCTASAGSLAFSGTNG
                     DESTYAAGVLLAVGGLLVLLTRRPRTNARRRS"
     gene            4575..5747
                     /locus_tag="VFX21_00875"
     CDS             4575..5747
                     /locus_tag="VFX21_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HEX5094527.1"
                     /translation="MAQTEVAPEKKERWSFSWKHLYDEVVTSGLCTGCAGCVIACPHD
                     VLSYDDLHGHYKPVQIEDYGGAGDCSHGDKACTSCTRACPRFRAWEPEIDNFMFGRTR
                     TVDEPSGIFKDICLARAADGELHEVGQDGGLVSALLVYALEHDIVDAALVSYLEGDGS
                     SWKAIPGIARTRAEVIASAGSRYTYSANTLAYMDLAKEDERIALVGMSCQSSVPPAMK
                     QRKAGKVARRLSLNIGLLCSKTFDDAIFEELFQAKYGLEKKNIKKMNIKGVFQIWTHD
                     GEYHEIPLKECHAWTREGCNMCPDFAAEHADISTGGIGKYNDWTLTIVRTDAGRELME
                     RMLADGVIEVRPGDDDPDAIALMHKLSKKSRKRWPETAIPEPRLIPPPAPKAPTVG"
     gene            5817..6818
                     /locus_tag="VFX21_00880"
     CDS             5817..6818
                     /locus_tag="VFX21_00880"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX5094528.1"
                     /translation="MPRAQAVVQFLRTETAGGVALLAATVAAMVWINIAPVSYADTWQ
                     HKEWINDGLMLLFFFVVGLEIKREIVSGELRQPRTAALPAIAAVGGMVVPALLFLAVM
                     RGSDGAHGWGIPMATDIAFAVGVLALIGRGLAPGTKIFLLALAIVDDIGAIIVIALFY
                     GHGWGGARTVGAVALALCIPVRTRSGARPAERIEESLHPVVSFAIVPLFALANAGIRL
                     GGDALRDGFGSRLTWAVIIGLVVGKLIGVSGAATLAVRTRAGERPAGVGRRETAGIAA
                     LAGIGFTVALFVSELAYEGKAGQLSDAKLGIFVASLTSALIGSAILRAGSSRTSIEK"
     gene            6819..7472
                     /locus_tag="VFX21_00885"
     CDS             6819..7472
                     /locus_tag="VFX21_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5094529.1"
                     /translation="MGSTTVRVLLVEDHEMVARGLQAALQDEPDLEVVGLAASVEDGV
                     LSYKALHPDVVVMDYRLPDGAGTDATRRIRDLDDDAPVLLLTGADDPGVVTAALDAGC
                     SGFVTKDRGVDDLARAIRSVAHGAAVFPAGLLARALHSTPTGYEVGDDLTAREREVLE
                     ALANGRSTEELASDLFLSLHTVRNHVRNILTKLHARTKLEAVVIAARAGIVDLRPRP"
     gene            7469..>8263
                     /locus_tag="VFX21_00890"
     CDS             7469..>8263
                     /locus_tag="VFX21_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094530.1"
                     /translation="MSVDAGNKRRRFSSLRSRIGLVVVLAVIPGFVISIIAVNDERNS
                     SKDEAQRAAVTIAGSTAARYQSLLRESQSLLQVLRNIPSTVYDEEACSTVLKTVIEDS
                     ATYENMFILNADGQVVCGRDEDRGQPPIRADWFETALTQDTFVVAETEPGESPSYPLM
                     ITAYGVGEGTDRYVLASEFSLEPLGDVIGNSFPEGATVTMLDRDDVVMYRGPGPSEFI
                     GKSIRNTDIVQKLRDRGGEGVITARGTDDVQRLYAYRTLTQPDGAAI"
BASE COUNT         1413 a         2916 c         2726 g         1208 t
ORIGIN      
        1 cgagcgcggt ctcctccccg acacgcgtcg cttcgaccac caaccgcccc gacgtgttga
       61 ccgtcgcgcc gaacacggcg tcgccggccg cgacgtcgac cggcaccggt tctcctgtca
      121 gcatcgaaac gtcgaccgca ctcgcaccgc cgacgacgac gccgtcagtc gcgatcttct
      181 cgcccggtcg gacgacgaat cggtcgcccc gcaacaagct cgcgacggga acctcgtcgc
      241 cgttctcgag ccgtgccgtc tttgcgccga tcgcgagcag cgcgcgcagt gcagatcccg
      301 cgcggcttcg tgcacgcgct tcgaaatagc gaccgagcag gagcaaggtc gtgatcaccg
      361 ccgcggtctc gaagtagatc tccgcgccgt cgacgaacaa cgcggcgacc gaccagaagt
      421 acgcggccgc agttccgacc gagatcagtg tgtccatcgt cgtggtgcgg tggcgcgcgc
      481 cggcgagcgc aacgcgatgg aatggccagc cggcccacca cacgaccggc gtcgcgagaa
      541 gaagggccca cccgcgccat cccgcgaact gcaacggctc gatcatcgac accgcgagca
      601 acggcagggt gaggagcgcg gccggcacga ggcgccgcaa cacggcgcgc gcttcgtcgg
      661 catcgggatc gacggcttca atctccggga ccacgtagcc gaggtcggtg atcgcggtcg
      721 cgacgctggt cgggtcgagg tggccgggtt cgtagtcgac cgcggcccgg ccggtcgcga
      781 agttcacgcg cacggcgtcc acgccgggga gcttggccaa cgactgctcg atgcgggccg
      841 cgcacgacgc gcacgtcatc ccctggacgg ggacgtcgag cgtctgggtc gtcgtgacat
      901 ggtcctgcat gcgtgttact atacccccct accctattga aggccagagc ccggaggaca
      961 cggtgcgcgg ttacacgatg cacaaggacg actacctgaa ccgcctgcgc cgcatcgagg
     1021 gccaggtgcg cgggctccag cgcatggtca acgaggacga gtactgcatc gacgtcctca
     1081 cccagatcgc gtccgtctcc aaggcgctcc agggcgtggg cctcggcctg ttggacgagc
     1141 acctcagaca ctgcgtcacc gaagcggtcc actccggtga gggcgacgcc aagatccaag
     1201 aagcggttca agccgtagaa cgcctgctca gggcgtagcc gcacgacgcg ggtgccggcg
     1261 catgtcgcct atttcggtcc tatcggccga ttgccgcgcg gagacgtcgc cagctaccct
     1321 ccgtgaaccg gggtcggcgt gtggggcgcc aaggagcgga gagggagggg accagtgcgg
     1381 tcccagggga cgcggcgcgc aatctgggtg ctgccggcgc tgctgttggt cgccggcgcc
     1441 gcgctggcgc gcggcccgtc cgcgccggca ccggccgcgg tcgacctcgc ggtgcgcggc
     1501 agcgtcgaac agatctcggt cacccacgca acgcccggtg cgccactcca gctgcgcgac
     1561 gcgggcaacg acgtcgtggg cgaagccacc gtcgacgacc tgggcagtgt gctgtggcgc
     1621 aacgtcgcgc ccggagacgg gtacgaggtc gtcgagctcg acggcaccac gaccggcccg
     1681 gtccacgtgc tcgcgcagga cgagctcccg gcgcagtcgc tctacgccaa ccaagagctg
     1741 cacgacggct tccaatacat cacgacgcgc gacggcacga agctcagtgt cagcgtgaag
     1801 ttgcccggtc ccgcggcgaa cggcccctac cccaccgtcg tcgagtactc cggctatgcc
     1861 gcgtcgcagc cgaacacgcc gcaaccgagc acgcagatcg cgcagctgct cggctacgca
     1921 accgtggcgg tcaacgtgcg cggcaccgga tgctccggcg gtgcgttctc gtacttcgaa
     1981 ccgctgcagg gactcgacgg ctacgacgtg atcgagaccg tcgccgcgca gccgtgggcg
     2041 ttgcacggca aggccggcat gatcgggatc tcgtatcccg gcatcagcca gctgttcgtt
     2101 gcgcgtacgc agccgccgca cctggccgcg atcgcaccgc tgtcggtcat cgacgacacg
     2161 taccgcagca cgctctatcc gggcggcatc ttcaacaacg gcttcgcgct cagctgggcg
     2221 aaggagcgca tggaccaagc cgcgccgtac ggccaagggt gggaacaagg tgtcgtcgac
     2281 gccggcggcg cgaacggcca gcagtgcgcg gagaaccagc tcctgcgcca ccagaaccag
     2341 aacctgctcg ccgagatctc ggcgcacccg ttctacgacc cggcactcgg cgacgcgctc
     2401 tcgccggtga ccttcgccga caagttcgac acgccggtgt tcatcggcgg cgcgtggcag
     2461 gacgaacaga ccggcggaca cttcacggcc atgtggtcga agttcgcgac caaggcgaag
     2521 atgttcggca cgaacggcac gcacgtcgac agcctgctcg ccgagctcaa ccggtggtac
     2581 gagttcctcg agttctacgt cgccgaacgc acgccgcagg tcccgacgct cgtccgccag
     2641 gccgcgccgc tgttggtgca gagccagatc ggcatcggcg gctatcagct cgaacccgac
     2701 cgcttcacgg gctaccccac cttcgccgag gcgctcgccg cgtacgaggc cgagccgccg
     2761 gtgcgcatcc tcatggagaa cggggccggc gatgctgcga atcccggttc gccggtcggc
     2821 acgttcgaga tgggattcgc gtcgtggccg ccgccgcaga cgcaagccac gacgtggttc
     2881 ttccaacccg accagcacct cacggcgagc gcgcccacag tcgccgacga cgacggccgc
     2941 gcgtccacgt cgtacgtcta cgacccgacc gcgaaacccg cgaccgactt ctcgggctcg
     3001 tcgtccgaca tctggcaggc gacacccgac tacgactgga agccgctccc gatcggcaag
     3061 gcgctggcct tcgacagcga cgccttgacc gcggagacgg tcatggccgg taccggcagc
     3121 gtcgacctct ggttgcgctc gacggcagcc gacaccgacc tggaggtgac gctcaccgaa
     3181 gtgcgccccg acggccagga gatgtacgtg cagagcggtt ggctgcgcgc gagccaccgc
     3241 gcgctcgacc ccgcgcagtc caccgacttg cggccgtggc acacccacgc cgaagccgac
     3301 gcggaaccgt tgccgaaggg ggagttcgtc tccgcgcgcg tcgagctgtt cccgttcgcg
     3361 cacgtgttcc acgccggctc gaagttgcgc atcaccgtcg aagcaccggg cggcaaccgg
     3421 ccgttgtgga cgttcgacgc gctcgacgcc gacggcacgg tcgtcaacga catcgcccac
     3481 tcggtcggtc acccgtcgaa ggtcgtgctg cccgtgatcc ccggcgtcga cgcaccggac
     3541 gcgctcccgc cgtgcccgtc gctgcgcggt cagccctgcc gcactgccct cggcgcggcg
     3601 caaccgatcg gcgtgcaggc tgacgtgacg gggcaggacg cgaccgtgac atggacaccg
     3661 ccgcccgcgc gtcccgacgt caccgtcgag agctaccgcg tgaccgaagc gcccgggggc
     3721 gcaaccaagg atgtcggcgc caacggcgcc gcaacgccgg cggccacgag cgccgcaccg
     3781 ctctccgtgc agttcacggg tctcgccaac ggcacacatt ccttcagcgt cgaagcgcgc
     3841 tacgcgcaag gtggcagcag cggaccgtcg acgccgtcga actcggtgac cgcggccgcg
     3901 caagaggagt cgacgaccac gaccactgcc acgccgacga cgacaacgac cgtcgcaccc
     3961 ctgaactcgg ccgacgcgac gacgagcacg accgaatccg acgacgagct cgacgacgac
     4021 ttcgccgacg acgagtcggg cgacttcagc gacgacacga gcgacgactt cagcgacgac
     4081 ttctcggacg acttcagcac tgatccgtgc gcgccgaccg acgacttcag tgaccccacg
     4141 ttcgacgacc ccacgaccga cacgaccgcc gtcgacgact tcgacgactt cgacgacttc
     4201 ggcaccgagg acctgtcacc ggatccgtcg gagctgtccg acgaccccga cttcatgctc
     4261 cacggcacac aggagaccga cgagctcact cccgaagacc tcggcctcac gcaggaagag
     4321 ttcgaccagc tctacggcac gggcgacacg acggacacga ccgacccgtt ctcgagtacc
     4381 gacgacttct cgaccaccga cgacttcagc agcgatccct gcaccgcgag cgcgggcagc
     4441 cttgccttca gcgggacgaa cggcgacgaa tccacatatg ccgcgggcgt tctcctcgcg
     4501 gtcggcggcc tgctcgtgct cctgacccgc cggccccgga ccaacgcgcg gcgccggtcc
     4561 taaactgcgc cgccatggcg caaaccgagg tcgcaccgga gaagaaggaa cgctggtcct
     4621 tctcgtggaa gcacctctac gacgaggtcg tcacgtcggg cctgtgcacc ggttgcgcgg
     4681 ggtgcgtgat cgcgtgcccc cacgacgtct tgtcgtacga cgacctccac ggtcactaca
     4741 agccggtcca gatcgaagac tacggcggcg cgggcgactg cagccacggc gacaaggcgt
     4801 gcacgtcatg cacccgcgcc tgcccacgct ttcgggcgtg ggagcccgag atcgacaact
     4861 tcatgttcgg ccgcacccgc accgtcgacg agccgtcggg catcttcaag gacatctgcc
     4921 tggcccgcgc ggccgacggc gagctgcacg aggtcggcca ggacggcggg ctcgtctccg
     4981 cgctgctcgt gtacgcgctg gagcacgaca tcgtcgacgc cgcgctcgtg tcgtacctcg
     5041 aaggcgacgg ctcgtcgtgg aaggccatac ccgggatcgc gcgcacccgc gccgaagtca
     5101 tcgcgtccgc gggtagccgt tacacgtact ccgcgaacac gctcgcgtac atggacctcg
     5161 ccaaggaaga cgaacgcatc gcgctcgtcg gcatgtcgtg ccagtcgtcg gtcccgcccg
     5221 cgatgaagca acgcaaggcg ggcaaggtcg cgcggcgact ctcgctcaac atcggcttgc
     5281 tgtgctcgaa gacgttcgac gacgcgatct tcgaagagct gttccaagcg aagtacggct
     5341 tggagaagaa gaacatcaag aagatgaaca tcaagggcgt gttccagatc tggacgcacg
     5401 acggcgagta ccacgagatc ccgttgaagg aatgccacgc gtggacccgt gagggctgca
     5461 acatgtgtcc cgacttcgcg gccgagcacg ccgacatctc gaccggtggt atcggcaagt
     5521 acaacgactg gaccctgacg atcgtgcgga ccgacgccgg ccgggagctg atggaacgca
     5581 tgctcgccga cggcgtcatc gaggtgcggc ccggcgacga cgatcccgac gcgatcgcgc
     5641 tgatgcacaa gctgtcgaag aagtcgcgca agcgctggcc cgagaccgcg attcccgagc
     5701 cgcgtctgat cccgccgccg gccccgaaag cgcccaccgt cgggtaatct cgctccccgg
     5761 agcgccggga agtctggtcg gcatcgaact catccgccct tgagacggga tgtgcgatgc
     5821 cgcgtgcaca ggcagtcgtc caatttctgc gcactgagac cgccggcggc gtcgcgctgc
     5881 ttgccgcgac cgtcgccgcg atggtctgga tcaacatcgc acccgtttcg tacgccgaca
     5941 cctggcaaca caaggaatgg atcaacgacg ggttgatgct cctgttcttc ttcgtcgtcg
     6001 gcctcgagat caagcgcgag atcgtcagcg gcgagctacg gcagcctcgt accgcggcgc
     6061 ttcccgccat tgccgcggtc ggcggcatgg tcgttcccgc gctcctcttc ctcgcggtga
     6121 tgcgtggcag cgacggcgcg cacggttggg gcatcccgat ggcgaccgac atcgcgttcg
     6181 cggtgggcgt gctcgcgctc atcggtcgcg gcctcgcgcc gggaacgaag atcttcctgc
     6241 tcgcgctcgc gatcgtggac gacatcggtg cgatcatcgt gattgcgttg ttctacgggc
     6301 acggttgggg cggcgctcgc acggtcggtg cagtggcttt ggcgctgtgc atcccggtgc
     6361 ggacccgatc cggcgcccgc ccggcggagc ggatcgagga gtcgttgcac ccggtcgtga
     6421 gcttcgccat cgtgccgctg ttcgcgctcg ccaacgccgg catccgtctc ggcggcgacg
     6481 cgctccggga cgggttcggc agccggctga cgtgggcggt gatcatcggc ctggtcgtcg
     6541 gcaagctcat cggcgtcagc ggggcggcca cgctcgccgt ccggacccgg gccggtgagc
     6601 gcccggccgg cgtgggacgg cgcgaaaccg ccgggatcgc ggccctcgcc ggcatcgggt
     6661 tcacggtcgc cctttttgtg tccgaactgg cgtatgaggg taaagccgga caactgagtg
     6721 acgccaagct cggcatcttc gtagcgtcgt tgacgagcgc cctcatcggg agtgcgattc
     6781 tcagagcggg aagcagtcga acgtcgatag agaagtagat gggctccaca acggtgcgtg
     6841 tcctcctcgt ggaggatcac gagatggttg cacgcggcct gcaggctgcg ctgcaagacg
     6901 agcccgacct cgaagtcgtc ggcctggcgg catcggtcga ggacggcgtg ctgtcctaca
     6961 aggcattgca ccccgatgtc gtcgtcatgg actaccgcct ccccgacggc gccggcaccg
     7021 acgccacgcg ccggatccgc gacctcgatg acgacgcacc cgtcttgttg ctcaccggcg
     7081 ccgacgatcc gggggtggtg acggccgccc tcgacgcggg ctgcagtgga ttcgtcacca
     7141 aggaccgcgg cgtcgacgat ctcgcacgcg cgatccgctc ggtcgcgcac ggcgcggcgg
     7201 tgtttcccgc gggactgctc gcacgcgcgc tgcacagcac gccgaccggc tacgaggtgg
     7261 gcgacgacct cacggcacgc gagcgcgaag tcctcgaggc gctcgccaac ggacgttcga
     7321 ccgaggagct cgcgtccgat ctgttcctct cattgcacac ggtgcgtaat cacgtccgca
     7381 acatcctcac gaagttgcac gcacgcacga agttggaggc ggtggtcatc gcggctcgag
     7441 cggggatcgt ggacttgcga cctcgcccat gagcgtcgat gcggggaaca agcggcggcg
     7501 cttctcgtca ctccgatcgc gcatcgggct cgtcgtcgtg ctcgcagtca tccccggttt
     7561 cgtcatctcg atcatcgcgg tcaacgacga gcgcaactcg agcaaggacg aagcgcaacg
     7621 ggcggcggtc acgatcgcgg ggtcgacagc cgcgcgctac cagtcgctgc tgcgcgagag
     7681 ccagtcgttg ttgcaggtgc tgaggaacat tccgtcgacg gtctacgacg aggaggcctg
     7741 ctccaccgtc ctcaagacgg tgatcgagga ctccgcgacg tacgaaaaca tgttcattct
     7801 caacgcggac ggtcaggtcg tctgcggacg tgacgaggat cgcgggcaac caccgatcag
     7861 ggctgattgg ttcgagaccg cgctcacaca agacacgttc gtcgtggccg agacggagcc
     7921 cggcgagtcg ccgagctatc cgctgatgat cacggcgtac ggcgtcggcg agggcaccga
     7981 ccggtacgtc ctcgcttcgg agttctcact cgagccgctc ggcgacgtca tcggtaactc
     8041 gttccccgag ggcgcaaccg tcacgatgct cgaccgcgac gacgtcgtca tgtatcgcgg
     8101 gccggggccg agcgagttca tcggcaagag catccgcaac accgacatcg tccagaagct
     8161 gcgcgaccgc ggcggcgaag gcgtcatcac cgcgcgcggc accgacgacg tgcaacgcct
     8221 ctacgcgtac cggacgctca cccagccgga tggcgctgcc atc
//
LOCUS       DASUGA010000046         2664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_404442, whole genome shotgun sequence.
ACCESSION   DASUGA010000046 DASUGA010000000
VERSION     DASUGA010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404442"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VFX21_00895"
     CDS             complement(<1..615)
                     /locus_tag="VFX21_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5094531.1"
                     /translation="MEHLRFETRDGVAIVTLVDVERRNAMTATMVREIVETFDTIEAD
                     DSIGAVVVTGHPPAFCSGADVSSLGALADARSDEERTGVVSIYEGFLRVLRSTRPTVA
                     AVNGPAVGAGMNLALACDVRLAGASARFDARFLKIGLHPGGGHTWMLERAVGPQAAAA
                     IVLFGEAIDGARAAEVGLAWRCHPDAELLDAAVTFAQRAAKVPAP"
     gene            complement(620..1648)
                     /locus_tag="VFX21_00900"
     CDS             complement(620..1648)
                     /locus_tag="VFX21_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5094532.1"
                     /translation="MLVTGLSTYWGGRVAQVLEQRDDVEVVVGVDTKDPTVPLERTEF
                     VRADSSYSILNRIVRATEIDTILHTHLVVDSTASTSRSLHEINVIGTMNLLAAAGAPD
                     SPVRKVVLKSSALVYGANYQDPNQFREDMTRTRPARTAVERSLLEVEAFLRDFADDNP
                     HISVTLLRFANVLGDDIDTPFVKALRRPIVPEILGFDPRVQFVHEDDVVGALIYATRN
                     DVPGVYNVAGDGNLPWSEVCMIVGKRRIPLPPVLTSYAAEPPRFLRLWDLPAEALSLL
                     RYGRSVDNDRYKKAGFRYRYTSAGTVEAFARGLRLSGAIGETHPAYRYEREVEDFFRH
                     SPAVVRPE"
     gene            complement(1656..2624)
                     /locus_tag="VFX21_00905"
     CDS             complement(1656..2624)
                     /locus_tag="VFX21_00905"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5094533.1"
                     /translation="MVRFPGLVNDDPDRPRDVVDLTERSRVDVTAGVVVEPGRQLVPV
                     PERPQPEARLNTTDGDPARQSDVDKWGRSEHIRSLTRRLYDPLYKYWFRVEWEGFDRL
                     PEGGGALLVSNHAGAIPSDAPAIMHGIETELGRPVYGLAEYLFRAMPAIGTMWSRVGG
                     VPAHPDNAYRLLHDERQLVLVFPEGTKGTGKHFSQRYQLRRFGRGGFVEIAMRSGVPV
                     IPLAVVGAEESMPILWKSNRLAKMMNIPYFPVTANMLALGPLGLAGYFPAKFKIRVLP
                     PVHFDVLPNQERYSRSRVMEESERIRKMVQEALYDMLRTRKSVWFG"
BASE COUNT          426 a          886 c          928 g          424 t
ORIGIN      
        1 cggcgcgggg accttcgcgg cgcgctgcgc gaacgtgacg gcggcgtcga gcagctccgc
       61 atcggggtgg cagcgccacg cgagcccgac ctccgcggcg cgggcgccgt cgatggcctc
      121 cccgaacagc acgatggcgg ccgcggcctg cggaccgacc gcgcgctcga gcatccaggt
      181 gtggccgccg ccgggatgga gtccgatctt caagaaccgc gcgtcgaagc gggccgacgc
      241 gccggccagg cggacgtcgc acgcgagcgc gaggttcatg cccgcgccga cggccggtcc
      301 gttgacggcc gcgaccgtcg gccgcgtcga ccgcagcacg cgcaggaatc cttcgtagat
      361 ggacaccact ccggtgcgtt cttcgtcgct gcgcgcgtcg gcgagcgccc cgaggctcga
      421 cacgtcggcg cccgagcaga acgcgggcgg atgaccggtg acgacaacgg caccgatgct
      481 gtcgtcggcc tcgatcgtgt cgaacgtctc gacgatctcg cgcaccatcg tcgccgtcat
      541 cgcattgcgg cgctcgacgt cgacgagggt cacgatcgcg acgccgtcac gcgtctcgaa
      601 ccgcaggtgt tccacgttgc tactccggtc gtacgactgc gggtgagtgg cggaagaagt
      661 cctcgacttc tcgctcgtag cgatacgccg ggtgcgtctc tccgatcgcg ccggacaggc
      721 gcagaccccg ggcgaacgct tcgaccgtgc ccgcgctcgt gtaccggtag cgaaagccgg
      781 ccttcttgta acggtcgttg tcgacactgc gcccgtagcg caacagcgac agcgcctcgg
      841 ccggcagatc ccacaggcgt aagaagcgcg gcggttcggc cgcgtacgac gtcagcacgg
      901 gcggcagcgg gatgcgacgc ttgccgacga tcatgcagac ctcgctccag ggcaggttgc
      961 cgtcgccggc gacgttgtag acgcccggga cgtcgttgcg cgtcgcgtag atcaacgccc
     1021 cgacgacatc gtcttcgtgc acgaactgca cgcgcggatc gaacccgagg atctcgggga
     1081 cgatcgggcg gcggagcgct ttcacgaacg gcgtgtcgat gtcgtcgccg agcacgttcg
     1141 cgaaccgcaa caacgtgacc gagatgtgcg ggttgtcgtc ggcgaagtcg cgcaggaacg
     1201 cctccacttc gagcaaggag cgttcgacgg cggtgcgagc ggggcgcgtg cgcgtcatgt
     1261 cctcgcggaa ctggttgggg tcctggtagt tggcgccgta gacgagcgcc gagctcttca
     1321 agacgacctt gcgcaccggg ctgtccggtg cacccgccgc ggccagcagg ttcatcgtgc
     1381 cgatcacgtt gatctcgtgc agcgaccgac tcgtcgacgc ggtcgagtcg acgaccaagt
     1441 gggtgtgcag gatcgtgtcg atctccgttg cgcgcacgat ccggttgagg atcgagtacg
     1501 acgagtcggc ccgtacgaat tcggtgcgtt cgagcggcac cgtcgggtcc ttggtgtcga
     1561 cgccgacgac gacttcgacg tcgtcgcgtt gttcgagcac ttgcgcaacc cgtccacccc
     1621 agtaggtgct caagcccgtc accaacacgc gcatatcagc cgaaccagac gctcttgcgg
     1681 gtgcggagca tgtcgtagag cgcttcttgc accatcttgc gaatgcgttc cgactcttcc
     1741 atgacgcgac tgcgcgagta gcgctcctgg ttcgggagca cgtcgaagtg cacgggcggc
     1801 agcacgcgaa tcttgaactt cgccgggaag tagccggcca acccgagggg accgagcgcg
     1861 agcatgttcg cagtcaccgg gaagtagggg atgttcatca tcttcgcgag gcggttgctc
     1921 ttccacagga tcggcatcga ctcctccgca cccaccactg cgagcgggat gaccggcacg
     1981 ccggaccgca tcgcgatctc gacgaatccg ccgcggccga agcgccgcaa ctggtagcgc
     2041 tgcgagaagt gcttgcccgt gcccttcgtg ccctcgggaa atacgagcac gagctgccgc
     2101 tcgtcgtgga gcaaccggta ggcgttgtcg gggtgcgcgg gcacgccacc gacgcgtgac
     2161 cacatcgtgc cgatggcggg catcgcgcgg aacaagtact cggccaggcc gtacaccggg
     2221 cgaccgagct cggtctcgat cccgtgcatg atcgcgggcg cgtccgaggg aatcgcgccc
     2281 gcgtggttcg agacgaggag cgcgccgccg ccttcgggca gccggtcgaa tccttcccac
     2341 tcgacgcgga accagtactt gtagaggggg tcgtagaggc ggcgggtcag cgagcggatg
     2401 tgctcgctgc ggccccactt gtcgacgtcg gactgccggg ccggatcacc gtcggtcgtg
     2461 ttgaggcgcg cctcgggttg cggacgttcg ggcaccggca cgagttggcg gccgggttcg
     2521 acgacgacgc ccgcggtcac gtcgacccgc gatcgttccg tcaggtcgac gacgtcgcgg
     2581 gggcggtccg gatcgtcgtt caccaagcca ggaaaccgca ccaccggacg attttcaaac
     2641 tgggtgttcg gcgccgggta tcag
//
LOCUS       DASUGA010000047         3605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_961115, whole genome shotgun sequence.
ACCESSION   DASUGA010000047 DASUGA010000000
VERSION     DASUGA010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961115"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VFX21_00910"
     CDS             <1..413
                     /locus_tag="VFX21_00910"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094534.1"
                     /translation="VGDEREFGYWGEVLTTAAQARGIVGLVIDGGVRDVDALRRHDFP
                     AFSVMIALPGASKLRPGSVGTRARVGDVDVEMGDWVVGDADGVTVVPGAQLDAVLAAG
                     RAREAKEARMFDALRAGQTTIELLGLDGSSIERP"
     gene            complement(410..1360)
                     /locus_tag="VFX21_00915"
     CDS             complement(410..1360)
                     /locus_tag="VFX21_00915"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX5094535.1"
                     /translation="MGRVLVTRRLPDGGLDPLAGHDIVGPKDDDAPYTHEELCALAAE
                     VDAIVSLLTDRIDADVLVAGAGGGRLRCVANVAVGYDNVDIATAKFHNITVCNTPGVL
                     DETTADLAFLLILAASRLASTAEADLRGGRWHGWGITQYLGRDVHDAVLGIVGYGRIG
                     RAVAARAAGFGMRVIHHARTPTGVAGYVEDLDRLLAAADVVTLHVPGGDTTRHLIDGR
                     RLALMKATAVLVNTARGPVVDEEALATALHAGELFAAGLDVFEREPEVHPRLLAAPRT
                     VLLPHIGSASAATRTNMAQMATGAVVTVLEGGTPPNVVPG"
     gene            complement(1363..1752)
                     /locus_tag="VFX21_00920"
     CDS             complement(1363..1752)
                     /locus_tag="VFX21_00920"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5094536.1"
                     /translation="MDVRSIEQVEPVVEHNGTVPVWWLVSPREMFEITKGGYLELANE
                     FEVAGGSYVFPHEHPTHEFYYVTSGRGIMTIADESREISQGDLVYIPPDTVHSLKPIS
                     DTAPIHCFCFAVGVKDAPAINYTDHPE"
     gene            complement(1752..2135)
                     /locus_tag="VFX21_00925"
     CDS             complement(1752..2135)
                     /locus_tag="VFX21_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5094537.1"
                     /translation="MDVRSIEDVSPVVEHNGTVPVWWLVNPREMFDITNGGHLELVSE
                     FEVAGGGFVDPHHHPTHEFYYVTSGRGIMTIGDETREIRQGDLVHIPPNEVHSLRPVS
                     DTAPIHCFCFAVGVDGSGPVNYTEH"
     gene            2148..2648
                     /locus_tag="VFX21_00930"
     CDS             2148..2648
                     /locus_tag="VFX21_00930"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF016040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050385 - ureidoglycolate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HEX5094538.1"
                     /translation="MHILPLTAAAVTPEGFAPFGVLPPDEGDGNPTADLEFTRADGWV
                     NYIGHSLDEIEVVDGRYRCELLNRHDTHTQTLMPMSGPAVVVVAPAALDFSDAAHLSE
                     IRAFLLPQYACVHLLRGTWHWGPYPIGATTLRIFNIQGAGYPTDNGIAALKTDLATVV
                     DVEIQV"
     gene            2717..3439
                     /locus_tag="VFX21_00935"
     CDS             2717..3439
                     /locus_tag="VFX21_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094539.1"
                     /translation="MTAITTLDKEIEREERIVGIRPDGPVTRAKISTRQAQVALVLVL
                     VSVALLGVAFSPQGTTYLRVLTVALAVALSLYALEQDRHLRRLSVLTNDSQRIALAVV
                     DALTGSGALKSDADVLHVRESFERAAGSIASGLAEVLLADIARVRIAGPSGEVPIAAV
                     YCGLFGAPDDPTIAQHALRRGQPLRRTVDGRTTLAVPVWHHDQPIAILEVISPLGVVY
                     APQDAALVEAYARGALAARRAP"
     gene            3451..>3605
                     /locus_tag="VFX21_00940"
     CDS             3451..>3605
                     /locus_tag="VFX21_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5094540.1"
                     /translation="MGELDQQIAVVTGGGSGIGRATVEVLAREGATVAVFDRDAGAAR
                     NAAGPVNG"
BASE COUNT          582 a         1256 c         1208 g          559 t
ORIGIN      
        1 acgtcggtga cgagcgtgag ttcggctatt ggggcgaggt gctcaccacc gccgcgcagg
       61 cacggggcat cgtcggcctg gtgatcgacg gcggcgtgcg cgacgtcgac gcgctgcggc
      121 ggcacgactt tcccgcgttc agcgtgatga tcgcgttgcc gggcgcctcg aagttgcggc
      181 cgggatcggt gggcacgcga gcgcgcgtcg gcgacgtcga cgtggagatg ggggattggg
      241 tcgtcggcga tgccgacggc gtgacggtcg tccccggcgc acagctcgac gcggtcctcg
      301 cagcgggacg ggcacgcgag gcgaaggaag cgaggatgtt cgacgcgttg cgcgccggcc
      361 agacgaccat cgagctcctc ggcctcgacg gctcgtcgat cgagcggccc taacccggaa
      421 cgacgttggg cggggtaccg ccctcgagca ccgtcacgac cgcacccgtg gccatctgcg
      481 ccatgttggt gcgcgtcgcc gcggacgcgg aaccgatgtg cggcaacaac acggtgcggg
      541 gcgcggccaa caaccgcgga tgcacttcgg gttcgcgctc gaacacgtcg agcccggccg
      601 cgaacaactc acctgcatgg agcgcggttg ccaacgcctc ttcgtcgacg accggcccgc
      661 gcgcggtgtt caccagcacc gcggtcgcct tcatcaacgc cagccgccgc ccgtcgatca
      721 ggtgccgggt tgtatcaccg cccggtacgt gcagcgtcac gacatcggcc gcggccagga
      781 gccgatccaa gtcctcgacg tagccggcaa cacctgtcgg cgtgcgcgcg tggtggatca
      841 cacgcatccc gaacccggcc gcacgcgccg caaccgcgcg tccgatgcgg ccgtagccga
      901 cgatgccgag caccgcgtcg tgcacgtcac ggccgaggta ttgcgtgatg ccccaaccgt
      961 gccagcgccc gccgcgcagg tcggcttccg cggtcgacgc caaccgcgac gccgccagga
     1021 tcaacaagaa cgcgaggtcc gcggtcgtct cgtcgagcac gcccggcgtg ttgcacaccg
     1081 taatgttgtg gaacttcgcg gtcgcgatgt cgacgttgtc gtagcccacg gccacgttcg
     1141 cgacacaccg cagtcgccca ccgcccgcac ccgcgacgag cacgtcggcg tcgatgcggt
     1201 ccgtcagcaa cgagacgatc gcgtcgacct cggccgcgag cgcgcacaac tcctcgtgcg
     1261 tatacggcgc gtcgtcgtcc ttcggaccga cgatgtcgtg acccgcgagc ggatcgagac
     1321 cgccgtcggg gagacggcgc gtcaccagga cacggcccat cgctattcgg gatggtcggt
     1381 gtagttgatc gccggcgcgt ccttcacgcc gaccgcaaag cagaagcagt ggatcggcgc
     1441 agtgtcgctg atcggcttca agctgtgcac cgtgtcgggc gggatgtaca cgagatcgcc
     1501 ctgcgagatc tcgcgcgact cgtccgcgat cgtcatgatg ccccggcccg atgtcacgta
     1561 atagaactcg tgggtggggt gctcgtgcgg gaacacgtac gaaccgcccg cgacctcgaa
     1621 ctcgttcgcg agctccaggt acccgccctt ggtgatctcg aacatctcac gcgggctcac
     1681 caaccaccac accggcacgg tcccgttgtg ctcgacgacg ggctcgactt gctcgatcga
     1741 acggacgtcc atcagtgctc cgtgtagttg acgggcccgg agccgtcgac acccactgcg
     1801 aagcagaagc agtggatcgg cgcggtgtcg ctgaccggac gcaggctgtg cacctcgttg
     1861 ggcgggatgt ggacgagatc gccctggcgg atctcgcgcg tctcgtcgcc gatcgtcatg
     1921 atgccgcggc ccgaggtcac gtaatagaac tcgtgcgtcg gatgatggtg cgggtcgacg
     1981 aacccgccgc ccgccacttc gaactcgctc accagttcga ggtggccgcc gttggtgatg
     2041 tcgaacatct cgcgcgggtt cacgagccac cagaccggaa ccgtgccatt gtgttcgacg
     2101 accggactga cgtcctcgat cgaccgtacg tccatagggc caagcgtatg cacattctcc
     2161 cgttgaccgc agcggcggtg acgccggagg ggttcgcacc cttcggcgtg ctgccccccg
     2221 acgaaggtga cggcaacccg accgccgacc tcgagttcac ccgcgccgac gggtgggtca
     2281 actacatcgg gcacagcctc gacgagatcg aggtcgtcga cggccgttac cgctgtgaac
     2341 tgctgaaccg gcacgacacg cacacccaaa cgctcatgcc gatgagcgga cccgcggtcg
     2401 tcgtggtcgc gccggccgcg ctcgacttct cggatgccgc gcacctgtcc gagatccgcg
     2461 cgttcctgtt gccgcagtat gcgtgtgtac acctgcttcg aggaacgtgg cactgggggc
     2521 cgtatccgat cggtgcaaca acgctgcgga tcttcaacat ccaaggtgcc ggctacccga
     2581 ccgacaacgg catcgccgcg ctcaaaacgg acctcgcgac cgtcgtcgac gtcgaaattc
     2641 aagtctgacc cctcgatctt tcgacaatcc gaagtgaaac gcaatgcggg gacctgatcg
     2701 agggggtagg tccaccatga cggccatcac gacactcgac aaagagatcg agcgggaaga
     2761 gcgcatcgtc ggtatccgcc ccgacggtcc ggtcacccgg gccaagatca gcacgcggca
     2821 ggcccaggtc gcgctggtgc tcgtgctcgt gtccgtggcg ttgctcggtg tcgcgttctc
     2881 accgcaagga acgacgtatc tgcgcgtcct cacggttgcg ctcgcggtgg cgctgtcgct
     2941 gtacgcactc gaacaggacc gccatctgcg gcgcctctcg gtgctcacca acgactcgca
     3001 gcgcatcgcg ctcgccgtcg tcgacgcgct gaccggctcc ggcgcgctga agagcgacgc
     3061 cgacgtgttg cacgtccgcg aatcgttcga acgcgcggcc ggcagcatcg catccggtct
     3121 ggccgaggtc ttgctggccg acatcgcccg cgtgcgcatc gcggggccat ccggcgaagt
     3181 accgatcgcg gccgtctact gcggcctctt cggcgctccc gacgatccga cgattgcgca
     3241 acacgcgctg cgccgagggc agccgctgcg gcggaccgtc gacggtcgca cgacgctcgc
     3301 cgtgccggtc tggcaccacg accagccgat cgcgatcctc gaagtgatct cgcccctcgg
     3361 tgtcgtctac gcgccccagg acgcggcgct cgtcgaggcc tacgcgagag gagcgctggc
     3421 cgcgcgccgc gcgccgtagt ctcccggcgc atgggagagc tcgaccagca gatcgccgtc
     3481 gtgaccggcg gcggttcggg gatcggacgc gcgacggtcg aggtactcgc gcgagaaggt
     3541 gcgacggtcg cggtgttcga ccgcgacgcg ggcgccgcgc gcaacgccgc cggcccggtc
     3601 aacgg
//
LOCUS       DASUGA010000048         4059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_821294, whole genome shotgun sequence.
ACCESSION   DASUGA010000048 DASUGA010000000
VERSION     DASUGA010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821294"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VFX21_00945"
     CDS             <1..788
                     /locus_tag="VFX21_00945"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX5094541.1"
                     /translation="GSALTYVGAAISLFAACPVPVRPDNAFEVSLAGSFVNRITPAGV
                     GGIGLNLRFMQKAGANTTEAASRWGVNALIGGVVHIVLTALFVLWAGKDGAFDFHLPE
                     MPLLVAFGAVCVGAGVIFLTPFGRKKLLGPATGVAAHAWEGLRGVAHQPSRLCLMVLG
                     SVLVTMSYTLSLYASVRAFGGTTSLAAVGAVYLTGSVVSKAAPTPGGLGAVEAILIAG
                     LVSAGLAKVIAVPAVLLYRLCTFWIPILPGWGAFARLSRVEII"
     gene            795..1442
                     /locus_tag="VFX21_00950"
     CDS             795..1442
                     /locus_tag="VFX21_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5094542.1"
                     /translation="MGAERNVAVGRSRSLRVRISVAFVAAASLLALTLLVDKHRVADR
                     EVDVDRWFNDAPAWVAHALWPVMQLGLVWAPIVVGLVAAWFYGWRRGLAVVVAGLAGW
                     FLAKQVKHLVERGRPLRYIPDVNVREGAGTGLGFVSGHTTIAFAMATVLAPVLPRWGR
                     VVAFALATLVGVARVVYGAHLPLDVVGGACLGIVIGCVVDLVLLALPLSRNEERA"
     gene            complement(1416..1970)
                     /locus_tag="VFX21_00955"
     CDS             complement(1416..1970)
                     /locus_tag="VFX21_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011534997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5094543.1"
                     /translation="MTETRTRASFTSFAESTAEDWAIIMGQDDTAEIAAIADRALWLL
                     RQMDEYGGFPVTRLEHSVQTATRAERAGEDDEYIVCALLHDIGDTLAPYNHASIAAEM
                     LRGFVSEENLFMVEHHGEFQGYYFWHHIGGDRDARDRFRGSPHFDRAAEFCEKYDQCA
                     FDPDYESEPLEHFEPLLRAFFVPR"
     gene            complement(2064..3233)
                     /locus_tag="VFX21_00960"
     CDS             complement(2064..3233)
                     /locus_tag="VFX21_00960"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX5094544.1"
                     /translation="MFDAVGKIEAGMSSLDHDFEADALTGHDALALLRRLAAIRARVD
                     GLTAKLAKRVDDTCAYLSTGDRDAEHTVSRVLGVELGAAKQAIETATRLESLPSVDAA
                     VRAGELSARQAQLIAETATHNPDATAELIAVARQGLDPLKDACVAARAAVEDADERSK
                     RQHRERSFRMRTSSDGMIRGSFALTPEIGGQIKAIVDKGVQQIFRERRAGAEHEPHQA
                     YAADVFANAFLQPAELPAAKVTTHVVIDHSVLAVGDALPGATCEIPGVGPVNAQWARD
                     LLGESFLTFVIKKGKDITTVAHFGRHIPAELRTALVVSGRECDVEGCHNRGYLEIDHS
                     HPVAKGGVTSWQNLRWLCYVHHKRKTQGYEFARKRPQPVAEPSRARQRVLAAADP"
     gene            3402..>4059
                     /locus_tag="VFX21_00965"
     CDS             3402..>4059
                     /locus_tag="VFX21_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094545.1"
                     /translation="MPFFEPEPPPPTQSFEDERAQNWAPPLWDRPSEGTYPGVVTVGQ
                     LFGVTENTAFGLDAVRAYPNGFVLDTTLLVNPRLPPSQNPRGLGFRLGGVALTAHTAT
                     GVSADKGESKAPPEPPANPFRLMWPRIGVQFADGRRAGDANSMSQFNRSQARDDLGIP
                     TDIVIGPMGGGGGSHGFRFGTWIFPLPPPGPLDIYAEWSTIGLQESLITLDGGAVIDA
                     A"
BASE COUNT          578 a         1398 c         1376 g          707 t
ORIGIN      
        1 tgggttccgc gctcacctac gtgggcgcgg cgatcagcct gttcgcggcg tgcccggtgc
       61 cggtgcgtcc ggacaacgcg ttcgaggtca gcctcgcggg atcgttcgtg aaccgcatca
      121 cgccggccgg tgtcggtggc atcggtctga acctgcgctt catgcagaag gcgggtgcga
      181 acacgaccga ggcggcgtcg cgctggggtg tcaacgcgct gatcggcggt gtcgtccaca
      241 tcgtgctgac cgcgctgttc gtgttgtggg ccggcaagga cggcgcgttc gacttccacc
      301 tgccggagat gccgctgttg gtcgcgttcg gtgcggtgtg cgtcggcgcg ggcgtcatct
      361 tcctcacgcc gttcggccgc aagaagctgt tgggcccggc caccggggtc gccgcgcacg
      421 cctgggaagg actgcgcggc gtcgcccacc agccgtcgcg gctgtgcctg atggtgctcg
      481 gcagcgtctt ggtgacgatg agctacacat tgtcgttgta cgcgtcggtg cgcgccttcg
      541 gtgggacgac gagcctcgcc gcagtcggcg cggtgtatct gaccggttcg gtggtcagca
      601 aggccgcgcc gacaccgggc ggcctgggcg cggtcgaggc gatcctgatc gcgggcctcg
      661 tgagcgccgg gctcgcgaag gtgatcgcgg tgcccgcggt gctcctctac cggctgtgca
      721 cgttctggat cccgatcctg cccgggtggg gcgcgttcgc acggctgtcg cgcgtcgaga
      781 tcatctgacc cggcgtggga gccgagcgaa acgtcgcggt cgggcgctca cgatccctgc
      841 gggtacggat ctccgtcgcg ttcgtcgccg ccgcgtcgtt gctcgcgctc acgttgctcg
      901 tcgacaagca ccgcgtcgcg gaccgggagg tcgacgtcga ccggtggttc aacgacgcgc
      961 cggcgtgggt tgcgcatgcg ctgtggccgg tcatgcagct cggcctggtc tgggcgccca
     1021 tagtcgtcgg gctggtcgcc gcgtggttct acggatggcg acgcggtctc gccgtcgtcg
     1081 tggccggcct ggccgggtgg ttcctggcca agcaagtgaa gcacctcgtc gagcggggcc
     1141 gcccgctgcg ctacatcccc gatgtcaacg tgcgcgaggg tgccggcacc gggctcggtt
     1201 tcgtctcggg ccatacgacc attgccttcg cgatggcgac ggtcctcgcg ccggtgctgc
     1261 cgcgttgggg ccgcgtcgtc gcgttcgcac tggccacgct cgtcggcgtc gcccgcgtcg
     1321 tctacggcgc gcacctgccg ctggacgtcg tcggtggcgc gtgcctcggc atcgtcatcg
     1381 gctgcgtcgt cgacctggtg ctgctcgcgc tgccgctatc gaggaacgaa gaacgcgcgt
     1441 aacagcggct cgaagtgctc cagcggttcg ctctcgtagt cggggtcgaa cgcgcactga
     1501 tcgtacttct cgcagaactc ggcggctcgg tcgaagtgcg gcgaccctcg aaacctgtcg
     1561 cgtgcgtcgc gatcgccacc gatgtgatgc cagaagtagt agccctggaa ctcgccgtgg
     1621 tgctcgacca tgaacaggtt ctcttccgac acgaacccgc gcagcatctc ggccgcgatc
     1681 gacgcatggt tgtagggcgc gagcgtgtcg ccgatgtcgt gcaacaacgc gcacacgatg
     1741 tactcgtcgt cttcgccggc ccgctcggca cgcgtcgcgg tctggacgct gtgctccagc
     1801 cgcgtgaccg ggaaaccgcc gtactcgtcc atctgccgca gcagccacag cgcccggtcc
     1861 gcgatcgccg cgatctcggc cgtgtcgtcc tggcccatga tgatcgccca gtcctcggcg
     1921 gtggactcgg caaaggacgt gaagctcgca cgtgtgcgcg tctcggtcat gctcgacctc
     1981 ccgtgacgca ccagcgtacg tcagaccggt tgcgcctgcc tcagttgtca aggagctgct
     2041 ggggaactga acgcgcgtga atgtcaggga tcggcggcgg cgagcacccg ttgtcgcgcg
     2101 cgcgacggtt cggccaccgg ttgcggccgc ttgcgcgcga actcgtaccc ctgggtcttg
     2161 cgcttgtggt gcacgtagca caaccatcgc aggttctgcc acgacgtgac gccgcccttc
     2221 gcgaccggat gcgagtggtc gatctcgagg tatccacggt tgtggcatcc ctcgacgtcg
     2281 cactcccgac cgctcacgac caacgcggtg cgcaactccg ccgggatgtg ccggccgaag
     2341 tgggcgacgg tcgtgatgtc tttgcccttc ttgatcacga acgtcaggaa cgactcgccg
     2401 agcaggtcac gcgcccactg cgcgttcacc ggcccgacac ccgggatctc gcatgtcgcg
     2461 cccggcaggg cgtcgccgac ggccaggacg ctgtggtcga tcacgacatg cgtcgtgacc
     2521 ttcgccgcgg gcagctccgc gggctgcagg aacgcattgg cgaacacatc ggcggcgtag
     2581 gcctggtgcg gctcgtgctc cgcaccggcg cgccgctcgc gaaagatctg ctgcacgccc
     2641 ttgtcgacga tcgccttgat ctgaccgccg atctcgggcg tgagcgcaaa cgagccgcga
     2701 atcatcccgt cgctgctcgt gcgcatgcgg aacgaccgct cgcgatgctg acgcttcgat
     2761 cgctcgtcgg catcctcgac cgcggcgcgt gcggcaacgc acgcatcctt caacgggtcg
     2821 agcccctgac gcgcgacggc aatgagctcg gccgtcgcgt cgggattgtg cgtcgcggtc
     2881 tccgcgatca actgcgcctg ccgagccgac aactcacccg cgcgcaccgc ggcatcgacc
     2941 gagggcagcg actccaggcg ggttgcggtc tcgatcgcct gcttcgcggc gccgagctcg
     3001 acacccaaga ccctcgagac cgtgtgctcg gcgtcgcggt caccggtgct cagatacgcg
     3061 cacgtgtcgt cgacgcgctt cgccagcttc gcagtgagac cgtcgactcg cgcccggatc
     3121 gccgcgagcc gccgcagcaa cgccagcgcg tcgtgacccg tcagcgcatc ggcttcgaaa
     3181 tcgtgatcga gcgagctcat ccccgcctcg atcttcccca ctgcatcgaa cacgtgttcg
     3241 atcatagccg ggggtgcgaa aagaaccaag gcatttcgac gtgattcgtc aagaaatctg
     3301 gcgacgagcg aaacgcggcc gcccgaccgc cggtgcggtt tgaggacccg gcccgacaaa
     3361 agaaaggccg gcgacgcgcc gcgtcgttca tcatgtgacg catgccgttc ttcgagccgg
     3421 agccaccgcc gccgacgcaa tcgttcgagg acgagcgggc gcagaactgg gcgccgccgc
     3481 tctgggaccg tccgtcggag ggcacctatc ccggcgtggt gaccgtcgga cagctcttcg
     3541 gcgtcaccga gaacaccgcg ttcggcctcg acgcggtgcg cgcctatccg aacggcttcg
     3601 tgctcgatac aacgctcctt gtcaatccgc ggctgccacc gtcgcagaat ccgcgcggac
     3661 tcggcttccg actcggaggc gtcgcgctca ccgcgcacac ggcgaccggt gtctcggctg
     3721 acaagggcga gagcaaagcg cctcccgaac cgcccgccaa tccgttccgt ctgatgtggc
     3781 cgcgcatcgg cgtgcagttc gccgatggtc gacgcgccgg cgacgccaat tcgatgagcc
     3841 agttcaatcg gagccaggca agagatgatc tcggcatccc gaccgatatc gtcatcgggc
     3901 cgatgggagg tggaggcggt tcccacggct tccgcttcgg cacgtggata ttcccattgc
     3961 caccccccgg cccgctcgac atctatgccg aatggtcaac gatcgggctg caagagtcgc
     4021 tcatcacgct cgacgggggc gcggtcatcg acgccgcgc
//
LOCUS       DASUGA010000049         2376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_960818, whole genome shotgun sequence.
ACCESSION   DASUGA010000049 DASUGA010000000
VERSION     DASUGA010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960818"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VFX21_00970"
     CDS             complement(<1..370)
                     /locus_tag="VFX21_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003938685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HEX5094546.1"
                     /translation="MSPVDVATPMSERSIPIGDYGLIGDTRSAALVAPDGSIDWWCVP
                     RFDDPPVFGRLVGGEKAGWFSLAPRDAATPPRRAYREGTATLMTTWAVDGGELELADS
                     MVAEVTGRFLPGTVLVRRVSS"
     gene            complement(370..846)
                     /locus_tag="VFX21_00975"
     CDS             complement(370..846)
                     /locus_tag="VFX21_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010594167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094547.1"
                     /translation="MDWFGWATFGLVATTGLTASMIAAQLTGRTRLDLPLMLGTMRTD
                     DPDRARVVGFAIHLAIGQVFAFFYIAGFAALDRSQWWLGALFGLAHAAVALIVLVPLL
                     QGVHPRMASERAGPNSTAVLEPPGLFARNYGTTTPMFAVAAHVVFGAVLGLFVGSS"
     gene            946..2208
                     /locus_tag="VFX21_00980"
     CDS             946..2208
                     /locus_tag="VFX21_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012786850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5094548.1"
                     /translation="MPDDRTFLIVGASLTGAKAAETLRAEGFEGRVLLVGDERSRPYE
                     RPPLSKTYLRGESDLEKVFVHPDGFYAEHGIELRSDTTVTEIDRERHEAVLGDDERIA
                     YDRLLVATGAEPRRIDGPGAALENIHYLRNLDDADLLRAGLAPATSVAVVGGGWIGAE
                     VAASARQLGHSVTMIHSDPAPLVQVLGPEVAEVYRALHAEHGVELRMSTRVESFRGGT
                     AVEEVVTEKGDAIVADLVVVGIGVRPRTQLAIAAGLDVDNGIVVDQYLRTSDECVYAA
                     GDVASAWHPFFGRRVRVEHWSNALNQGRVAALNMLDLTTPYDRLPYFFSDQYDAGMEY
                     LGHAPEWDRVVFRGDPRAREFIAFWVKDDRVVAAMNMNVWDVVEPLRAIIHSRRPIAD
                     AQLRDTDVPLEEIATGLTAAASAPGSGA"
BASE COUNT          424 a          798 c          793 g          361 t
ORIGIN      
        1 gagaactcac gcggcggacc aagaccgtgc ccgggagaaa gcgacccgtg acctccgcca
       61 ccatgctgtc ggccagctcg agctcacccc cgtcaaccgc ccacgtcgtc atcagtgtcg
      121 ccgttccttc gcgatatgcg cgtcgaggcg gtgtcgcggc gtcgcgcggc gcgagcgaaa
      181 accacccggc cttctcgccg ccgacgagcc gaccgaacac gggtgggtcg tcgaagcgcg
      241 ggacgcacca ccagtcgatc gagccgtcgg gcgcgaccag tgcagcggat cgcgtgtcgc
      301 cgatcaatcc gtagtccccg attgggatgc tgcgctcact catcggcgtc gcgacgtcga
      361 ccggcgacat cagctgctcc cgacgaacag gccgagtacg gcaccgaaga ccacatgcgc
      421 agcgacggcg aacatcggcg tcgtcgttcc gtagttgcgt gcgaagagtc ccggcggttc
      481 gagcaccgcc gtggagttcg gcccggcgcg ctcgctcgcc attcgagggt gaacgccctg
      541 cagcagcggc acgagcacga tgagcgcgac cgccgcgtgc gccaggccga acagcgcgcc
      601 gagccaccac tgggaacggt cgagcgcggc gaagcccgcg atgtagaaaa acgcgaagac
      661 ctggccgatg gcgaggtgga tcgcgaagcc gaccacccga gcgcggtcgg ggtcgtccgt
      721 cctcatcgtg ccgagcatca acggcaggtc gagacgcgtg cggccggtga gctgcgcggc
      781 gatcatcgac gccgtcaggc cggtggtcgc gacgagccca aacgttgccc agccgaacca
      841 gtccatcgac gtgttctcca gtacgacttc taaatcgagc tgaccttgac gttactgatc
      901 tggtgccgta acgtcaatat cagttgccga tagaaggacc gaccaatgcc cgatgaccgc
      961 acattcctga tcgtcggtgc gagcctgacc ggcgccaagg cggccgagac gcttcgcgcg
     1021 gaaggcttcg aggggcgcgt tctcctcgtc ggcgacgagc ggtcgcggcc ctacgagcgg
     1081 cctcccttgt cgaagacgta cctgcgcggc gagagcgacc tcgagaaggt cttcgtccac
     1141 cccgacggtt tctacgcgga gcacggcatc gagctgcgtt ccgatacgac cgtgaccgag
     1201 atcgatcgtg agcgacacga ggcggtactc ggcgacgacg aacggattgc gtacgaccgt
     1261 ctccttgtcg cgaccggcgc cgaaccgcgg cgcatcgacg gcccgggcgc cgcactcgag
     1321 aacatccact acctgcggaa tctcgacgac gccgatctac tccgcgccgg gctcgccccg
     1381 gccacgagcg ttgcggtcgt cggcggcggc tggatcggag cagaagtcgc ggcctcggcg
     1441 cggcaactcg gacacagcgt cacgatgatc cactccgacc ctgcgcctct ggtgcaagtg
     1501 ctgggtcccg aggtcgccga ggtgtaccgg gcgctgcacg ccgagcacgg cgtggagttg
     1561 cgcatgagca ctcgtgtgga gtcgttccgc ggcggcacgg ccgtcgagga ggtcgtgacc
     1621 gagaagggtg acgccatcgt ggccgacctc gtggtcgtgg ggatcggcgt cagaccacgc
     1681 acacaactcg cgatcgcagc gggtttggac gtggacaacg gcattgtcgt ggatcagtac
     1741 ctccgtacga gcgacgagtg cgtatacgcg gccggagacg tcgcctcggc gtggcatccg
     1801 ttcttcggcc gtcgggtacg tgtcgagcat tggagtaacg cgctcaacca agggcgagtg
     1861 gcggcgctca acatgctcga tctcacgacg ccgtacgacc ggttgccgta cttcttctcc
     1921 gatcagtacg acgccgggat ggaatacctc ggtcatgccc ccgaatggga ccgtgtcgtg
     1981 ttccggggcg acccccgcgc ccgcgagttc atcgcgttct gggtcaagga cgaccgcgtc
     2041 gtggctgcaa tgaacatgaa cgtctgggac gtcgtcgaac cgctgcgcgc catcatccac
     2101 tcccgccggc cgatcgccga cgcgcagttg cgggacacgg acgtgccact cgaagagatc
     2161 gcgaccggct tgaccgccgc ggcgtcggca ccaggcagtg gcgcatgacc aagcttcagc
     2221 agcttcacga cgaacaaggc cagagcgcgt ggctcgacaa tctcacgcgt ggacacttgt
     2281 gccgcaggga gctgcgccga ttgatcgacg gcggcatccg gggcgtcacg tcgaacccga
     2341 ccatcttcgc gaaggcaatc agcggctcga cggact
//
LOCUS       DASUGA010000050         2889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1656133, whole genome shotgun sequence.
ACCESSION   DASUGA010000050 DASUGA010000000
VERSION     DASUGA010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656133"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VFX21_00985"
     CDS             <1..256
                     /locus_tag="VFX21_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094549.1"
                     /translation="AEDAGLPAVETPAPTTPAPAPAPVAETTSPAPAPADEGTVGILP
                     GEQPEQIIETPAPAPAPADEGTVGILPGEQPEYIITVPPE"
     gene            complement(271..2232)
                     /locus_tag="VFX21_00990"
     CDS             complement(271..2232)
                     /locus_tag="VFX21_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5094550.1"
                     /translation="MPPVELNDVEARVLGCLMEKAVTTPDSYPLSLNALVTACNQTTN
                     RDPIVRYDDNEVEHALEALREKGLSRRVKATGQRVVKHRHVADEGLEINAGEFAVIGV
                     LLLRGAQTPGELKSRTERWHRFRSLDDVEEVLQRLATRGFTRQLPRRPGQKESRWAQL
                     LTGEPAVEPPAYAAPAATTPAPSTDADATPATVPVAAPAEREAARPHSIDVRDPSTGE
                     SIRTVAITEAGEIGQKLERARRAQPAWAARAYEERAAALRAFRDLLEAETDECAAVTS
                     REMGKSVKEATNEIRAVLDRIDWNVDNVGRVIAPRSVAGCPPGMEERITCEPVGVVAH
                     VSAWNYPYFVALNSIVPALLAGNAVLYKPSELATLTGLRLVDLLHRAGVPVDVVHVVV
                     GDGMTGAELVDSDVDMVCFTGSHATGQKVARAVANRLVRLQLELGGKDAAYVCDDVDV
                     VNAAIAVAEGAFYNCGQSCSATERVYVHNAIWDQFVEAFVEAVADTDVGPMARAGQLE
                     VLEEQIEGAVRAGAKIAWGGSRVERPGNWFEPTVLLDVDDRMAIMRDESFGPVIALAR
                     VHDDEEAVRRMDDTDYGLGASVFTADRARAERILARLDVGNAYWNTADRSTVRLPWSG
                     RRHSGLGVSMSESGVRTFVREKAWHLHGS"
     gene            complement(2234..>2889)
                     /locus_tag="VFX21_00995"
     CDS             complement(2234..>2889)
                     /locus_tag="VFX21_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016334043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5094551.1"
                     /translation="VLAREAPVDYQGKHYKMPLPGGTGLGKPLKSITHPLRADIPIYL
                     ASEGPKNVALTAEIADGWLALFYGPRVDGFYREALTEGFARPSARRTPDDFEVACIVP
                     VIVHDDVETAANFYRPMLALYIGGMGARDQNFHNNVFTRMGYEAEAKLIQDLYLDGKK
                     AEAAAAVPTRLVEDTALIGPKEKIRDDLEMWRESIVTTLLVSGGPAQLREIAELVLG"
BASE COUNT          419 a         1049 c          955 g          466 t
ORIGIN      
        1 ggccgaagat gcgggcctgc ccgcggtcga gacgcccgcg ccgacgacac ccgcaccggc
       61 gccggcgccg gttgccgaaa ccacctcgcc cgcaccggcc ccggcggacg agggcaccgt
      121 cggcatcctc ccgggcgagc agcccgaaca gatcatcgag acgccggccc ccgcgcccgc
      181 gccggcggac gagggcaccg tcggcatcct ccccggcgag cagcccgaat acatcatcac
      241 ggtgcccccg gagtaacacg ggcgtccgtg tcagctcccg tgcaagtgcc aggccttctc
      301 tctgacgaag gtgcgcacgc cggactcgga catgctgacc ccgaggccgc tgtggcggcg
      361 gccggaccac ggtaaccgca ccgttgaacg gtctgcggtg ttccagtacg cattgccgac
      421 gtcgaggcgc gccaggatcc gttccgcccg cgctcgatcg gcggtgaaca ccgacgcgcc
      481 caagccgtag tcggtgtcgt ccatgcgacg gaccgcttct tcgtcgtcgt gcacacgcgc
      541 gagggcgatc acggggccga acgactcgtc gcgcatgatc gccatgcggt cgtcgacgtc
      601 gagcagcacc gtcggctcga accagttgcc gggccgttcg acgcggctgc cgccccacgc
      661 aatcttcgcg cccgccctca ccgccccttc gatctgctcc tcgagcacct cgagctggcc
      721 ggcgcgtgcc atcggcccga cgtcggtgtc ggcgaccgct tcgacgaacg cttcgacgaa
      781 ctgatcccag atcgcgttgt gcacgtacac gcgctcggtt gcgctgcacg actggccaca
      841 gttgtagaac gccccttcgg cgaccgcgat cgcggcgttg acgacgtcga cgtcgtcgca
      901 cacgtacgcc gcgtccttcc caccgagctc cagctgcagc cgcacgagcc ggttcgccac
      961 ggccctcgcg accttctggc cggtcgcgtg cgacccggtg aaacagacca tgtcgacgtc
     1021 gctgtcgacc agttccgcgc ctgtcatccc gtcaccgacg acgacgtgca cgacgtcgac
     1081 cggcacaccg gcgcggtgca gcaggtcgac gagacgaaga ccggtcagcg tcgccagctc
     1141 cgacggcttg tacagcacgg cgttcccggc gagcagggcg ggcacgatcg agttcagcgc
     1201 gacgaagtac ggatagttcc acgcgctcac gtgcgccacg acgccgaccg gctcgcacgt
     1261 gatgcgctct tccatgccgg gcgggcagcc cgcgaccgaa cgcggcgcga tcacgcgacc
     1321 gacgttgtcg acgttccagt cgatgcggtc gaggaccgcg cgtatttcgt tcgtcgcttc
     1381 cttgaccgac ttgcccatct cgcgactcgt caccgcggcg cattcgtcgg tttccgcctc
     1441 gagcaagtcg cggaacgcgc gcaacgcggc cgcccgctct tcgtaggcac gcgcggccca
     1501 tgccggttgg gcgcgccggg cccgctcgag cttctggccg atctcgccgg cttcggtgat
     1561 ggcgacggtg cggatcgatt cgcccgtcga cgggtcgcgc acgtcgatcg agtgcgggcg
     1621 cgcggcctcc cgttcggccg gtgccgccac cggcacggtg gccggcgtgg cgtccgcgtc
     1681 cgtgctcggt gccggcgtcg tcgccgcggg cgcggcgtag gccggcggct cgaccgcggg
     1741 ctcgcccgtg agcaactgcg cccagcgcga ttccttctgc ccgggtcggc gcggcaactg
     1801 gcgggtgaag ccgcgagtcg ccagccgctg caacacttcc tcgacatcgt cgagcgaacg
     1861 gaagcggtgc caccgctcgg tgcggctctt caactcgccg ggcgtctgcg cgccgcgcaa
     1921 caacaacacg ccgatcaccg cgaactcacc cgcgttgatc tcgaggcctt cgtcggccac
     1981 gtggcggtgt ttgacgactc gttgcccggt cgccttgacc cggcgcgaca aacccttctc
     2041 gcgcaacgct tcgagtgcgt gctcgacctc gttgtcgtcg tagcgcacga tcggatcgcg
     2101 attcgtcgtc tggttgcacg cagtcacgag cgcgttcagc gacaacggat agctgtccgg
     2161 ggtcgtgacg gccttctcca tgaggcagcc gagcacgcgc gcctcgacgt cgttcagctc
     2221 tacgggaggc atgtcatccg agcaccaact cggcgatctc gcgcagctgt gcggggccgc
     2281 ccgagaccag caacgtcgtc acgatcgatt cgcgccacat ctcgaggtcg tcgcggatct
     2341 tctccttcgg cccgatcaag gcggtgtcct cgacgagccg ggtcgggacc gcggcggcgg
     2401 cttcggcctt cttcccgtcg aggtagaggt cttggatcag cttcgcctcg gcctcgtacc
     2461 ccatgcgcgt gaacacgttg ttgtggaagt tctggtcgcg cgcacccatg ccaccgatgt
     2521 acagcgcgag catcgggcgg tagaagttgg cggccgtttc gacgtcgtcg tgcacgatga
     2581 ccggcacgat gcacgcgacc tcgaagtcgt cgggtgtgcg gcgcgcactt gggcgcgcga
     2641 atccctcggt gagcgcctcg cggtagaagc cgtcgacgcg tgggccgtag aacaacgcga
     2701 gccagccgtc ggcgatctcc gcggtgagcg cgacgttctt cggcccttcc gacgcgaggt
     2761 agatcgggat gtcggcgcgc aacggatgcg tgatcgactt caatggcttg ccgagaccgg
     2821 tgccgcccgg caacggcatc ttgtagtgct tgccctggta gtcgaccggc gcttcacgcg
     2881 cgagcacct
//
LOCUS       DASUGA010000051         2989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1656126, whole genome shotgun sequence.
ACCESSION   DASUGA010000051 DASUGA010000000
VERSION     DASUGA010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656126"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2936
                     /gene="mfd"
                     /locus_tag="VFX21_01000"
     CDS             <1..2936
                     /gene="mfd"
                     /locus_tag="VFX21_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX5094552.1"
                     /translation="RDELAAHLVAMGYRREYQVEARGEFAVRGSIVDVYPSTADHPVR
                     IDLWGDEVDRLSQFAVADQRSQADVDETWIFPVRELLPTDEVRARAAELLTEQPWGRE
                     HWERLAEGQMFDGMESWLPWLSPGEHLLPDLLSNEALVALVEPRRLRDRAQELLDEEA
                     SLATTLASTWGASQTEDAPRLSLPFDRLVANTGAGTALLLATPDNPSTPALAAGAFDP
                     VVGDSEGLARRINALKADGFKILVAAEGSGSKTRIEQMLAEEGAPADVVIAPIDRGAV
                     FPSIKLALIAEADLTGRRRVHRTPRGARKGADYYEGLGANDYVVHRVHGVGRYLGMET
                     REMFGITRDRLVVEFKGGDRVYVDSEDIGLIRKYTGGEEPRLSKMGGADWEKTRARVR
                     AAVRDIAAELVILYRKRLATEGHAFAEDGPFQHQIEEAFPYEETPDQLKAIEETKADM
                     ERAIPMDRLICGDVGYGKTEVALRAAAKAVFDGRQVAVLVPTTLLASQHGQTFRERFA
                     NYPVRVEVLSRFLTTKEQNAVAKGVLDGQVDIVIGTHRLLSDDIMFKNLGLLIVDEEQ
                     RFGVQHKERIKTMRTNVDVLTLSATPIPRTLEMSLTGIRDLSLVQTPPEDRQPILTYV
                     GEFDPRAVSEAIRRELLREGQVLYVHNRVKDIEHVAADVRDLVPDARVAIAHGQMDEG
                     RLEKVVTQFWEHEHDVLVCTTIVESGLDMPTVNTLVVDRADLLGLAQMYQLRGRVGRR
                     GQRAYAYLLHPRDRALTEEAYERLKTIGEFTDLGSGFKIAMRDLEIRGAGNLLGGEQS
                     GHIAAVGFDTYVEMVTEAVGELTGETREEKAEVQIDVPITANLPRDYIARDDVRMEAY
                     RRLAAVTSHGDVDDVQAEWEDRYGPPPPSAAALLDVAHLRAECLRLGVRAVSVQKGAA
                     RFEGLELRKSQEARLKHLVARATVLADTVVVPVKGDGRALIDGMLRLLAEVVPADATP
                     VPSAS"
BASE COUNT          493 a         1047 c         1037 g          412 t
ORIGIN      
        1 accgggacga gctggccgcg cacctcgtcg ccatgggcta ccggcgcgag taccaggtcg
       61 aagcgcgcgg cgagttcgcg gtgcgcggct cgatcgtcga cgtctacccg tcgaccgccg
      121 accatccggt gcgcatcgac ctctggggcg acgaggtcga ccgactgtcg cagttcgcag
      181 tcgcggacca gcgatcgcag gccgacgtcg acgagacgtg gatcttccct gtgcgcgagc
      241 tcctgcccac cgacgaggtc cgcgcccgcg ccgccgagct gctgaccgag cagccgtggg
      301 ggcgcgagca ctgggagcgg ctcgcggaag ggcagatgtt cgacggcatg gagtcgtggc
      361 tgccgtggct gagtcccggc gagcacctcc tccccgatct cctgtcgaac gaagcactcg
      421 tcgcgctcgt cgagccccga cgactgcgcg accgcgccca ggaactgctc gacgaggaag
      481 catcgctcgc gacgacgctc gcgagcacgt ggggcgcgtc ccagaccgag gacgcgccgc
      541 gcctctcact gccgttcgac cgcctggtcg ccaacacagg cgcgggcacc gccctcctgc
      601 tggcgacgcc cgacaacccg agcacgcccg cactggccgc cggcgcgttc gacccggtgg
      661 tcggtgacag cgaaggactc gcgcgtcgca tcaacgcgct gaaggccgac gggttcaaga
      721 tcctcgtcgc ggccgaaggc agcggttcga agacgcgcat cgaacagatg ctcgcggaag
      781 aaggcgcgcc cgccgacgtc gtcatcgcgc cgatcgaccg cggcgcggtc ttcccgagca
      841 tcaagctggc gctgattgcg gaagcggatc tcaccggccg tcggcgtgtg caccgcacgc
      901 ctcgcggcgc gagaaaaggg gccgactact acgagggcct cggggccaac gactacgtcg
      961 tgcaccgcgt ccacggtgtc ggccgctatc tcgggatgga gacgcgcgag atgttcggca
     1021 tcacgcgcga ccggctcgtc gtcgagttca aaggcggcga ccgcgtctac gtcgattccg
     1081 aggacatcgg gctcatccgc aagtacaccg gcggcgagga gccgcgcctg tcgaagatgg
     1141 gcggcgccga ctgggagaag acccgcgccc gagtgcgcgc cgcggtgcgc gacatcgcgg
     1201 ccgagctcgt catcctgtac cgcaagcgcc tcgcgaccga aggtcacgcg ttcgccgagg
     1261 acggcccgtt ccagcaccag atcgaagagg cgttcccata cgaggagacg cccgaccagc
     1321 tcaaggccat cgaggagacg aaggccgaca tggagcgggc gatcccgatg gaccgcctca
     1381 tctgcggcga cgtcggctac ggcaagaccg aggtcgcgtt gcgggcggcg gcgaaggcgg
     1441 tgttcgacgg ccggcaggtc gcggtgctcg tcccgacgac gttgctcgcg agccaacacg
     1501 gccagacctt ccgtgaacgg ttcgcgaact atccggtgcg cgtcgaggtg ctctcgcgct
     1561 tcctcacgac gaaagagcag aacgccgtcg cgaagggtgt gctcgacgga caggtcgaca
     1621 tcgtcatcgg cacgcaccga ttgctctccg acgacatcat gttcaagaac ctcgggctgc
     1681 tgatcgtcga cgaggaacag cgcttcggtg tgcagcacaa ggagcgcatc aagacgatgc
     1741 gcacgaacgt cgacgtgctg acgctcagcg cgacaccgat cccgcgcacg ctcgagatga
     1801 gcctgaccgg catccgcgat ctctcgctcg tgcagacgcc gcccgaagac cgccagccga
     1861 tcctcaccta cgtcggcgag ttcgaccccc gtgcggtctc ggaggcgatt cgtcgggagc
     1921 tgctgcgcga gggccaggtg ctctacgtgc acaaccgcgt caaggacatc gagcacgtcg
     1981 cggccgacgt ccgggacctc gtccccgacg cccgcgtcgc gatcgcgcac ggccagatgg
     2041 acgaaggccg gctggagaag gtcgtcaccc agttctggga gcacgagcac gatgtgctcg
     2101 tctgcaccac gatcgtcgag agcggcctcg acatgccgac ggtcaacacg ctcgtcgtcg
     2161 accgcgccga cctcctcggc ctcgcgcaga tgtaccagtt gcgcggccgg gtcggccggc
     2221 gcgggcaacg cgcgtacgcg tacctcctgc atccgcgcga ccgcgcgctc accgaagagg
     2281 cgtacgagcg actgaagacg atcggcgaat tcaccgacct cggctcgggc ttcaagatcg
     2341 cgatgcgcga cctcgagatc cgcggcgcgg ggaacctgct cggcggcgag cagtcgggac
     2401 acatcgcggc cgtcggcttc gatacgtacg tcgagatggt caccgaagcg gttggtgagt
     2461 tgacgggcga gacgcgcgag gagaaggccg aagtgcagat cgacgtcccg atcacggcca
     2521 acctgccgcg cgactacatc gcgcgcgacg acgtccgcat ggaggcctac cgtcgcctgg
     2581 ccgcggtgac gagccacggc gacgtcgacg acgtgcaggc ggagtgggag gaccgctacg
     2641 gcccaccgcc gccatcggcc gcggccctgc tcgacgtcgc ccacctgcgc gcggagtgcc
     2701 tgcgcctcgg tgtgcgcgcc gtctccgtgc agaagggcgc cgcgcgcttc gaggggctcg
     2761 agctgcgcaa gtcgcaagag gcccggctga agcacctggt cgcccgcgcg acggtgctcg
     2821 ccgacaccgt tgtcgttccc gtcaagggcg atgggcgcgc gctgatcgac ggcatgctgc
     2881 ggttgctcgc cgaggtcgtt cccgcggacg cgacgccggt accatccgcg tcgtgatctc
     2941 acgcttcgcc cgcggtggtg tcgcggccgc gctcgtggcc gcggtcgcg
//
LOCUS       DASUGA010000052         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1513797, whole genome shotgun sequence.
ACCESSION   DASUGA010000052 DASUGA010000000
VERSION     DASUGA010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513797"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VFX21_01005"
     CDS             complement(<1..282)
                     /locus_tag="VFX21_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5094553.1"
                     /translation="MGKAVALVTGASAGLGVEFAKQLAQAGNDLVLVARDRDRLEQLA
                     KQLEADYGANAEVLTADLTDAAQLAGVEERVRTVDTLVNNAGYGTFGKFY"
     gene            complement(313..954)
                     /locus_tag="VFX21_01010"
     CDS             complement(313..954)
                     /locus_tag="VFX21_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HEX5094554.1"
                     /translation="MTKRKWMALGSALLIVLAAALTTAFVVNRDGEPDEYPMMVAPST
                     EKTTTTLKPTTTTAEPLPVPEAPPSDAYANVPVVQIGTIEIPSIGLVHPIYEGVTLTV
                     IDNGPGHWPGSAMPGKRGNTVFPGHRVTHSHPFLNLDLVKPGDQVVFHMPGEDAIYKV
                     SGTQIVVPTDMWVIDQTEKPTFTLIACHPKHSAAQRIVVKGDFVSKHKTTYKL"
     gene            complement(976..2043)
                     /gene="thrC"
                     /locus_tag="VFX21_01015"
     CDS             complement(976..2043)
                     /gene="thrC"
                     /locus_tag="VFX21_01015"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX5094555.1"
                     /translation="MTAPTSKRWTGIIDAYRDRLPVSEKTPVVTLNEGNTPLVDAPRL
                     SDAVRARVMLKVEGANPTGSFKDRGMTMAISKAAEEGAKVVVCASTGNTSASAAAYAA
                     RAGMLAAVIIPDGHVALGKLAQALIHGAKVVSIRGSFDDALHVVRELGERGGVTVVNS
                     INPYRIEGQKSAAFEICDALGAAPDVHCIPVGNAGNITAYWKGYTEYARDGVVANTPR
                     MLGWQAAGSAPLVLGEPVPHPETIATAIRIGNPASWDGATGARDDSGGHIGAVTDAQI
                     LDAYRFLASQEGVFVEPASAASVAGLLAAFELGLLAPDETIVCTVTGHGLKDPNRAIA
                     EIEARPAVDGTVDAVLEELGL"
BASE COUNT          356 a          739 c          680 g          407 t
ORIGIN      
        1 gtagaacttg ccgaacgtgc cgtacccggc gttgttcacg agcgtgtcga ccgtgcgcac
       61 gcgttcctcg acgcccgcga gttgcgcggc atccgtcagg tcggcggtaa gcacttcggc
      121 attcgcgcca tagtcggcct cgagctgctt ggcgagctgt tccaaccgat cgcgatcacg
      181 cgccaccagc acgagatcgt tgccggcttg tgcgagctgc ttcgcgaact cgacacccaa
      241 cccggcggac gcgccggtga cgagtgcaac ggccttgccc atgatgcccc ccttcggcaa
      301 tacgagcgtg cgttagagct tgtatgtcgt cttgtgcttc gagacgaagt cgcccttcac
      361 gacgatacgt tgcgcggcgc tgtgcttggg atggcacgcg atcaacgtga acgtgggctt
      421 ttccgtctgg tcgatcaccc acatgtcggt gggcaccacg atctgtgtcc cggagacttt
      481 gtagatcgcg tcctcgccgg gcatgtggaa caccacttgg tcgccgggtt tcacgagatc
      541 caggttcaag aacggatgcg aatgcgtcac gcggtgaccg gggaacaccg tgttgccgcg
      601 cttgcccggc atcgcgctgc cgggccagtg cccgggaccg ttgtcgatca ccgtaagggt
      661 gacgccttca tagatcgggt gcaccaaccc gatgctcgga atctcgatcg tgccgatctg
      721 cacgaccggc acgttggcgt aggcgtccga cggcggcgct tccggaaccg gcagcggctc
      781 ggccgtcgtt gtcgtgggct tgagcgtggt ggtcgtcttt tcggtcgacg gcgcgaccat
      841 catcgggtac tcgtcgggct cgccgtcgcg gttcacgacg aacgcggtcg tcagtgcggc
      901 cgccagcacg atgagcagcg ccgacccgag cgccatccat ttgcgcttgg tcatttcctt
      961 catacgatcc gacctctaca gcccgagttc ttccaacact gcgtcgaccg tgccgtccac
     1021 cgcgggacgg gcttcgatct cggcgattgc ccggttggga tccttcagcc cgtgaccggt
     1081 gaccgtgcac acgattgtct catcgggcgc aagcagcccc agctcaaatg ctgcaagcag
     1141 tccggcgacc gaagccgccg acgccggctc cacgaacacg ccttcttggg atgccaaaaa
     1201 ccggtaggcg tcgagaatct gtgcgtcggt gacggcaccg atgtggccgc cggagtcgtc
     1261 acgcgcgccg gtcgcaccgt cccagctcgc cggattcccg atccggatcg cggtcgcgat
     1321 ggtttccgga tgcggcaccg gttctcccaa caccagcggg gccgaccccg ccgcctgcca
     1381 cccgagcatg cgaggcgtat tcgcaacgac gccgtcacgc gcgtattcgg tgtagccctt
     1441 ccagtacgcg gtgatgttgc cggcattgcc caccggaatg cagtgcacat cgggtgccgc
     1501 acccaacgca tcgcagattt cgaaggcggc cgacttctga ccttcgatgc gataggggtt
     1561 gatcgagttc acgacggtga cgccgccgcg ctcccccagt tcgcgcacga cgtggagcgc
     1621 gtcatcgaac gaaccgcgaa tcgacacgac cttcgcgccg tggatcaacg cctgcgcgag
     1681 cttgccgagc gcgacatggc cgtcggggat gatgactgcc gcgagcatcc ctgcgcgcgc
     1741 cgcgtacgca gccgcgctcg cagacgtgtt gccggtggac gcacacacga caaccttggc
     1801 gccctcttcg gccgccttgg agattgccat cgtcatgcct cggtctttga acgaaccggt
     1861 cgggttcgcg ccttcgacct tcagcatcac gcgggctcgc accgcatccg agagacgcgg
     1921 agcgtcgacc agcggcgtat tgccttcgtt gagcgtcaca accggcgtct tctcgctgac
     1981 cggcaagcgg tcgcgatacg cgtcgatgat gccggtccac cgtttcgatg tcggcgcggt
     2041 cactcttcgc ctccgaccac acgcagcacc gagccgactg acaccacaac atcgaggtca
     2101 cgaacctcgt ggatcgtctt ggtcaacgcc gcttcccggg cgaggtgcgt cacgaagatc
     2161 aagcgggcgc cttcaccttg gc
//
LOCUS       DASUGA010000053         3000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2071222, whole genome shotgun sequence.
ACCESSION   DASUGA010000053 DASUGA010000000
VERSION     DASUGA010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2071222"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..1064
                     /locus_tag="VFX21_01020"
     CDS             60..1064
                     /locus_tag="VFX21_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018539151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX5094556.1"
                     /translation="MKLGLQLGYWGAQPNPFAAMLAQEAEKLGFDSVWTGEAWGSDAF
                     TPLAWIGAQTERIRLCTGVVQLSARTPAATAMHALTLDHLSNGRFTLGLGVSGPQVVE
                     GWYGQPFGKPLARTREYVEIVRRILARKEPVRFAGEHYRMPYDGPGSWGLGKPLRPIT
                     HPLRADLPILLGAEGPKNVALATEIADGWIPLYYSPLRPEVYGSLGETKPGFEVCVTV
                     MGCRITDDIEDALMTTKASLAFYIGGMGAKDRNFHMELMSRMGYEAEAHQIQELFFEG
                     KREEAVPLVPSAFADEISLVGPKERIRERLQAWEESPVTTMIVSGDLDTMRGIAELTL
                     "
     gene            complement(1174..1791)
                     /locus_tag="VFX21_01025"
     CDS             complement(1174..1791)
                     /locus_tag="VFX21_01025"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017565449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX5094557.1"
                     /translation="MKPYELPDLPYDYGALEPHYSARVLELHHDKHHAAYVTGLNTAL
                     EKLAAARDSGDMSTIVGLEKTLAFNLSGHVLHSLFWQNLSPAGGGKPEGELAAAIDED
                     FGSFDAFKAQLSEVTGTVQGSGWGALSWEPLGKRLFIEQIYDHQGNVGQSGVSLLVLD
                     AWEHAFYLQYENRKADYIKAIWNVVNWSDVAARFDRARNLQLIST"
     gene            1872..>3000
                     /locus_tag="VFX21_01030"
     CDS             1872..>3000
                     /locus_tag="VFX21_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019807480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5094558.1"
                     /translation="MTPDLVARLGEAVGASNVLSADAIPDDYAHDEALTATPVLPLAV
                     VRPGNAQEVAAVLRVASDLAVPVTARGSGTGLSGACIPRADGIVCSFERMQRIVEIDL
                     DNHVAVVEPGVTLDQLDEALAPHNLVYPVFPGEQSASLGGNVATNAGGMRAVKYGVTR
                     NQVLGLQAVLATGEVIRTGGKFVKATTGYDLTQLVIGSEGTLAIVTEATLKLYPRPPE
                     IATVLVPFATLEDVAGAVPHIVRSGIDPMVLEYVDGTGMTLLAANVGLDLGIPEEVQS
                     ACAAYLIVVVEGHHAERVEADLIRLADLVGELGALDVYVLPPRSGALLIEAREKAFWV
                     IKGMGANDIVDVVVPRASVPEYLAKVRAIGEEHGTFIGAAGH"
BASE COUNT          482 a          966 c         1054 g          498 t
ORIGIN      
        1 gagtgtcgcg catcggctcg cgttcgcagt gccatggcgg gctgtagtag tcagcgccca
       61 tgaagttggg cttacaactg ggatactggg gcgcgcagcc gaacccgttc gcggcgatgc
      121 tggcgcagga ggcggagaag ctcggcttcg actcggtgtg gaccggtgag gcgtgggggt
      181 ccgacgcgtt cacgccgctc gcgtggatcg gcgcacagac cgaacgcatc cggttgtgca
      241 ccggcgtcgt gcagctctcg gcgcgtacgc ccgcggcgac tgcgatgcac gcgttgaccc
      301 tcgaccacct gagcaacgga cgcttcacgc tcgggctcgg cgtgagcggc ccgcaggtcg
      361 tggagggctg gtacggccag ccgttcggga agccgttggc gcgcacgcgt gagtacgtcg
      421 agatcgtgcg ccgcatcctg gcgcgcaagg aaccggtgcg cttcgcaggc gagcactacc
      481 ggatgccgta cgacggcccc ggctcgtggg gactcggcaa gccgctgcgc ccgatcacgc
      541 atccgctgcg cgccgatctg ccgatcctgc tcggtgcgga aggcccgaag aacgtcgccc
      601 tcgcgaccga gatcgcggac ggttggatcc cgctgtacta ctcgccgctg cggcccgagg
      661 tgtacggctc gctcggcgag acgaagccgg gcttcgaagt gtgcgtgacg gtcatgggct
      721 gccgcatcac cgacgacatc gaagacgcgt tgatgacgac gaaggcgtca ctcgcgttct
      781 acatcggtgg catgggcgcc aaggaccgca acttccacat ggaactgatg agccgcatgg
      841 gttacgaagc cgaagcgcac cagatccagg agctcttctt cgagggcaaa cgtgaggaag
      901 cggtccccct cgtgccgagt gcgttcgcgg atgagatctc cctcgtcggc ccgaaggagc
      961 gcatccgcga acgactgcag gcgtgggagg agagcccggt gacgacgatg atcgtcagcg
     1021 gcgacctcga caccatgcgc ggcatcgcgg agttaacgct gtaggcccac gtcgcacgag
     1081 gcggtgccgg agcttgcgga ggcactcgcg gagtcctgag gccggccaac aatccgggct
     1141 gtctcacgcg agcaggtcga aggcgacaaa gagttatgtg gagatcagct ggaggttgcg
     1201 tgcacggtcg aagcgggcgg cgacgtccga ccagttgacg acgttccaga tggccttgat
     1261 gtagtcggcc ttgcggttct cgtactgcag gtagaacgcg tgctcccatg cgtcgagtac
     1321 cagcaacgac acaccgctct gaccgacgtt gccctggtgg tcgtagatct gttcgatgaa
     1381 cagccgcttg ccgagcggct cccacgacag cgcgccccag ccggaacctt gcacggtgcc
     1441 ggtgacttcg ctgagctgcg ccttgaaggc gtcgaagctg ccgaagtcct cgtcgatggc
     1501 ggccgcgagc tcgccttcgg gcttgccgcc gccggccggg ctcaggttct gccagaacaa
     1561 cgagtgcaac acgtgacccg acaggttgaa cgcgagcgtc ttctcgaggc cgacgatcgt
     1621 gctcatgtcg ccggagtcgc gcgcggcggc gagcttctcc agtgcagtgt tcaagcccgt
     1681 cacgtacgcg gcgtgatgct tgtcgtggtg caactcgagc acgcgggccg aatagtgcgg
     1741 ctcgagcgcg ccgtagtcgt agggaaggtc gggaagttcg tagggcttca tggtcatctc
     1801 ctaccccttt tcgtatcgag aaatgtcgta cggcttgacc gctgcggctc cgtcgtccta
     1861 catacggagc tgtgacgcct gatctcgtcg cccggctggg cgaagccgtc ggtgcctcga
     1921 acgtactgtc ggccgacgcg attcccgacg actacgcgca cgacgaagcg ctgaccgcga
     1981 cacccgtgct gcccttggcg gtcgtgcggc ccggaaacgc gcaggaagtg gctgcggtgc
     2041 tgcgcgtggc gtcggatttg gccgttccgg tgacggcccg cgggtcgggg acgggtctgt
     2101 cgggcgcgtg catcccgcgc gccgacggca tcgtgtgctc cttcgaacgt atgcagcgca
     2161 tcgtcgagat cgacctcgac aaccacgtcg cggtcgtcga gccgggcgtg acgctcgacc
     2221 agctcgacga ggcgttggcg ccgcacaatc tcgtctatcc cgtcttcccg ggcgagcaga
     2281 gcgcgtccct cggcggcaac gtggcgacca acgccggtgg catgcgcgcg gtgaagtacg
     2341 gcgtgacccg caaccaggta ctcgggctcc aggcggtgct cgcgacgggc gaggtgatcc
     2401 gcaccggcgg caagttcgtg aaggccacca ccggctacga cctgacccag ctcgtcatcg
     2461 gctccgaagg cacactcgcg atcgtcaccg aggcgacgct gaagctgtac ccgcgtccgc
     2521 ccgagatcgc gacggtgctg gtgccgttcg cgacgctgga agacgtcgcg ggcgcggtgc
     2581 cgcacatcgt ccgcagcggc atcgacccga tggtgctcga gtacgtcgac gggacgggca
     2641 tgacgttgct cgccgcgaac gtcggcctcg acctcggcat ccccgaggag gtgcagagcg
     2701 cgtgcgcggc gtacctcatc gtcgtggtcg aagggcacca cgcggagcgc gtcgaagcgg
     2761 acctgatccg tttggccgac ctcgtcggcg agctcggcgc gctcgacgtg tacgtcttgc
     2821 cgccgcgctc gggcgcgttg ttgatcgaag cgcgtgagaa ggcgttctgg gtcatcaagg
     2881 gcatgggtgc gaacgacatc gtcgacgtcg tcgtgccgcg cgcctcggtg cccgagtatc
     2941 tcgccaaggt ccgtgccatc ggcgaggagc acggcacgtt catcggcgcc gccggccacg
//
LOCUS       DASUGA010000054         2919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1793003, whole genome shotgun sequence.
ACCESSION   DASUGA010000054 DASUGA010000000
VERSION     DASUGA010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793003"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            625..2544
                     /locus_tag="VFX21_01035"
     CDS             625..2544
                     /locus_tag="VFX21_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094559.1"
                     /translation="MSDGDGGWVVAMTHEDATAGDARHCARIVHVRADGSLGWGVGAC
                     PYGVVNALTRLGPVVYVGGRFESIGNRARSNAGAVDIRTGKVTRWNPRVGGTRVYDRD
                     EIVPRQVNAIEAFDGRVLLGGFFDRVGGVRRVNFAVVGPREGKLLEPAIDVGDDEFGE
                     VRDIAVANGRAYLGGLFERVGRVVRTDAAAIDAETGRVLPWDARLRGGPVDAVAARPG
                     AILLAGRFTASRGLGRSGVMAASIATGAPLPWAPRIAARTSDWRVEDMLYVAGSVLVV
                     GDFVFVSHRMHPYAALIDGRGNLTAWDAGPAGRVSAAAYDGRRIALGGKFAGVGAVRR
                     TSLAALDVRTGRPRPWAPSVDGWVNALAVDRERLYVGGSFTRVGTAQRRDLAAFTRAT
                     LRLAPWRIDVAGQSVDALEVAGTTIFVGGDFDAIGGKPRSGVAAVDTSDAHLLGLDPQ
                     VEAMEDFTRPMVSSLLHAGRTVFAQGFVAPSGVVALDDETGEQRWAAVTDMSGVDAIA
                     LSGRRLVIVGFFTTVYSDTAGERRDGVAVLDRDTGDLLPWRIHPKTDYGVDYLGTSPG
                     VAANDRWVIVGGNFTSVDGKPRNGLAAVAASPSRVAAWHPLVGGVPVSHIAAPALYID
                     PNNDASLAVYTRRRR"
     gene            complement(2582..>2919)
                     /locus_tag="VFX21_01040"
     CDS             complement(2582..>2919)
                     /locus_tag="VFX21_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094560.1"
                     /translation="FRASIRGNVLELPDPAGGLTPDDLFVVSVASDLAWLARGFLRDR
                     DLADDVSVGASWRMPGNVSPLTELSLTIAVSVARQTAADALLAELEERVAPRSHGGTL
                     RVALVAGGL"
BASE COUNT          436 a          975 c         1057 g          451 t
ORIGIN      
        1 tgggtgccgc tggtggcgtg acgaagattc agacgctgcc gacctccgat tgggtgcgcg
       61 atgcttgtcg tcgcgttgcg ccgtggatgc gatgccccga gttcgaccgc cggcaacgta
      121 ggtaaatctc cacaattccg gtcggcgaga acgccgattt cgacgcgcgg gcatccgcgc
      181 agaatgaaag gcgtcgaacc gcgaagtcat gcgaccaggc cgacgcggtt ctgcggctgg
      241 aaggctgccc tccaggcgag gtgttccgac gacaccgcag gaatcgagga cgtgaatcac
      301 aagctcgcag cggcgattgt cgcggctgct gtgggggcga cggtgctcgc gacgacggga
      361 ttggtgtcgc cgggcgaggc acctagtcct ccgccttgcg gcagacaggc ccagttggat
      421 ccgacgaagt tgccatcgct gccgtcgagg caatgggcga cggacggcag cgtcgccgcg
      481 gtgatccctt gcggggacac ggtcttcgtg ggcggcggct tcaacgtcat cgggccgtac
      541 accggctcat ttgcatccgt ttctccgcgg tccggcacgg cggacccgcg cgcagctgcg
      601 ctccacagcc ccgtggacgc aatcgtgagc gacggcgacg gcggttgggt ggtcgccatg
      661 acgcacgagg acgcgactgc cggcgacgct cgtcactgtg ctcgcatcgt ccacgtgcgg
      721 gccgatggtt ccctcggttg gggagtcgga gcatgcccct acggcgtcgt gaacgcactt
      781 acgcgactcg gcccggtcgt ctacgtcggt ggacggttcg agagcatcgg gaaccgtgcc
      841 cgcagcaacg ccggggcggt tgacatccgt accggaaagg tcacgcggtg gaacccacgc
      901 gtcggcggaa cgcgcgtcta cgaccgtgac gagatcgtgc ctcgtcaggt gaacgcaatc
      961 gaggcgttcg acggtcgtgt gctcctgggc gggttcttcg acagggtcgg cggtgtgcgc
     1021 cgggtcaact tcgccgtggt tggcccgcgg gaggggaagc ttctcgagcc ggcgatcgac
     1081 gtcggcgacg acgagttcgg agaggtacgc gacatcgcgg ttgccaatgg acgcgcgtac
     1141 ctcggcgggc tcttcgaacg agtaggacgc gtggtgcgaa cggacgccgc ggcgatcgac
     1201 gccgagaccg gccgggtgct cccctgggac gccaggctgc gcggtgggcc ggtcgacgcc
     1261 gtggccgctc gccccggtgc gattcttctc gcgggtcgat tcacggcgag cagagggctg
     1321 gggcgttcgg gcgtgatggc ggcctcgatc gcaaccggtg cgccgcttcc ctgggcgccg
     1381 cggatcgcgg cgcgaacctc ggattggcgc gtcgaggaca tgctgtacgt cgcgggaagc
     1441 gttctcgtgg tcggcgattt cgtcttcgtc agccaccgca tgcatccgta cgccgcgctc
     1501 atagacggtc gtggcaacct gacggcctgg gatgccgggc ccgccgggcg cgtcagcgcg
     1561 gccgcatacg acggtcgtcg gatcgccctc ggcgggaagt tcgcgggcgt cggcgccgtt
     1621 cgacggacga gcctcgctgc actcgacgtg cgcaccggtc gaccgcgtcc gtgggcgccc
     1681 tccgtcgacg gctgggtgaa cgcgctggcc gtcgaccgcg agcgcctcta cgtcggaggg
     1741 tcatttaccc gagtgggcac cgcgcagaga agggacctgg ccgcatttac ccgggcaacg
     1801 ctgcggcttg cgccatggcg gatcgacgta gccggccagt ccgtcgatgc cctcgaggtc
     1861 gccggaacga cgatcttcgt cgggggtgat ttcgacgcga tcggagggaa gccccggtct
     1921 ggggtcgccg cggtcgacac gtcggacgca cacctcctcg gcctcgaccc gcaggtcgag
     1981 gccatggagg acttcaccag gccgatggtg tccagcctcc tgcacgcggg acgaaccgtc
     2041 ttcgcccagg gattcgtcgc gccgtccggt gtcgtcgctc tcgacgacga gaccggtgaa
     2101 cagcgctggg ccgccgtgac ggacatgagc ggcgtggacg caatcgctct ctccggcagg
     2161 cgactcgtca tcgtcggctt cttcaccacg gtctactcgg acaccgcggg ggagcgacgg
     2221 gacggggtcg ccgtcctcga ccgagacacc ggcgacctgc tgccatggcg gatccatccg
     2281 aagacggact acggagtcga ctacctcggg acatcacccg gcgtggcggc caacgatcga
     2341 tgggtgatcg tcggcggtaa cttcacgtcc gtcgacggga agccccgaaa cggcctggcg
     2401 gcggtcgcag cgagcccctc acgcgtcgct gcatggcatc cactcgtcgg aggcgtcccg
     2461 gtgtcgcaca tcgcggcgcc ggcgttgtac atcgatccga acaacgacgc ctcgctcgcc
     2521 gtctacacac gccggcgacg gtgagcattg ccgccgagat tcggcgacgg ccctcaccgc
     2581 gctacagccc tcccgcgacc agcgcgacgc gcagcgtgcc gccgtgcgac cggggagcga
     2641 cgcgctcctc gagctcggcc agcagggcgt cggccgccgt ttgccgggcc acggagacgg
     2701 cgatcgtcag gctcagctcg gtgagcggcg aaacgttccc gggcatccgc cacgacgcgc
     2761 cgacgctcac gtcgtcggcc aggtcacggt cccggaggaa cccgcgcgcc agccacgcca
     2821 ggtcggaggc gacggagacg acgaagaggt cgtcgggggt cagcccgccg gccgggtcgg
     2881 gcaactccag cacgttcccg cggatgctcg cgcggaagc
//
LOCUS       DASUGA010000055         2300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_123337, whole genome shotgun sequence.
ACCESSION   DASUGA010000055 DASUGA010000000
VERSION     DASUGA010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123337"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1735)
                     /locus_tag="VFX21_01045"
     CDS             complement(<1..1735)
                     /locus_tag="VFX21_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX5094561.1"
                     /translation="MSRFDEFGANSGFVEDLYQRYRDTPMSVDAQWRTFFAGQDDTPT
                     SASAEGPGPPEQAVSPDAVTPKAAPGPLQIEGDEPEPLRGSAARAVENMEASLTVPTA
                     TSARAVPAKLLEVNREILNNELERTGVGKVSFTHLIAFAVARALRDFPNLNASYELRD
                     GKPTVVRHNHLNLGLAVDVIRRDGSHSLLVPCVKAADTMDFAEFWRAYEALIEKVRSS
                     RVSPDDFAGTTATITNPGMIGTVHSVPRLMPGQGFILGVGRIGYPAEYEGADPHTLAE
                     LGIGRVMTLTNTYDHRIITGAESGEFLRRVHDVLIGDDRFYDDVFASFTVPYEPARWV
                     PDRGGFAEPASRDEKAIQVHSVINMYRVRGHLIAKLDPLARRPPTTHPELDITQHGLS
                     IWDLDREFPVGSFGAGLLGRKTMPLREILRVLRDAYARTIGVEYMHIQEPEQKAWIQE
                     RVETPRPPFTKDETRRILERLSAAEAFERFMHTKYLGQKRFSLQGAETLVPMVDALCT
                     EAARAGTTQIVFGMSHRGRLNVLANILGKSYEQIFREFEGELDPSSIEGTGDVKYHLG
                     ARGKHVSPDGRE"
BASE COUNT          348 a          734 c          795 g          423 t
ORIGIN      
        1 cctcgcgccc gtcgggggag acgtgcttgc cgcgtgcgcc gaggtggtac ttgacgtcgc
       61 cggtgccctc gatgctcgac gggtcgagct ccccttcgaa ctcgcggaag atctgctcgt
      121 acgactttcc caggatgttg gcgagcacgt tcaagcgacc gcggtgtgac attccgaaca
      181 cgatctgggt cgtgccggcg cgggccgctt cggtgcagag cgcgtcgacc atcggcacga
      241 gggtctccgc gccttggagg ctgaagcgct tctggcccag gtacttggtg tgcatgaacc
      301 gttcgaaggc ctcggccgcg ctgagtcgtt cgaggatgcg ccgcgtctcg tccttcgtga
      361 acggcggccg cggcgtctcg acgcgctcct ggatccaggc tttctgttcg ggctcctgga
      421 tgtgcatgta ctcgacgccg atggtgcggg cgtaggcatc gcggagcacg cgcaggatct
      481 cgcgcagcgg catggtcttg cgtccgagaa gtcccgcgcc gaagcttccg acggggaact
      541 cacgatccag gtcccagatc gagaggccgt gctgggtgat gtcgagctcg ggatgcgtgg
      601 tcggcgggcg ccgcgcgagc ggatcgagct tggcgatcag gtgtccgcgc acgcggtaca
      661 tgttgatgac cgagtgcacc tgaatcgcct tctcgtcgcg cgaagccggc tcggcgaacc
      721 cgccgcgatc ggggacccac cgggccggtt cgtacggcac ggtgaagctc gcgaagacgt
      781 cgtcgtaaaa ccgatcgtcg ccgatgagca cgtcgtggac gcgccgcaga aactccccgc
      841 tctccgcacc cgtgatgatg cggtggtcgt acgtgttcgt cagtgtcatg accctgccga
      901 tgccgagttc ggccaacgtg tgcgggtcgg cgccctcgta ctccgcgggg taaccgatgc
      961 ggcccacgcc gaggatgaac ccttgaccgg gcataaggcg cggcaccgag tggaccgtgc
     1021 cgatcattcc cgggttcgtg atcgttgcgg tcgtgccggc gaagtcgtcg ggggagactc
     1081 ggcttgaccg caccttctcg atcagtgcct catacgcgcg ccagaactcc gcgaaatcca
     1141 tcgtgtcggc tgccttgacg cacgggacga ggagcgaatg cgatccgtca cgacgtatga
     1201 catcgaccgc caacccgagg ttcaggtggt tgtggcgcac cacggtcggc ttcccgtctc
     1261 ggagctcgta cgacgcgttg aggttcggga agtcgcgcag tgcgcgcgcc accgcgaacg
     1321 cgatgagatg cgtgaagctc accttgccga cgccggtgcg ctcgagttcg ttgttgagga
     1381 tctcgcggtt gacctcgagc agtttggcgg ggacggcgcg tgccgatgtg gccgtcggga
     1441 cggtgaggct cgcttccatg ttctcgaccg cgcgtgcggc gctcccgcgc aggggctcgg
     1501 gctcgtcgcc ttcgatctgc agcggacctg gagccgcctt cggtgtgacc gcatccggtg
     1561 acacggcttg ttccggcgga ccaggccctt cggcgctcgc cgaggtcggc gtgtcatcct
     1621 ggcccgcgaa gaaggtgcgc cactgggcgt ccaccgacat cggtgtgtcg cgatagcgct
     1681 gatacaggtc ctcaacgaac cccgagttgg cgccgaactc gtcgaagcga ctcactcggt
     1741 catgatcgcg cgtcgcgact ggcccaccag tcgagatcgc ggagcccggt tcctgcacct
     1801 cacatggagg caagcgtgtc gtgagcaatc acctggaaga agccgcgagc cgacacgtcc
     1861 ggcaacgaga gagcgcaaca tcggatccgt cgctggaagg agtcgctgga agaatgggct
     1921 tggcttcatc ggtccccggg tataccgtgc gcgagttcga cgcaatcgtt gagtgcgctg
     1981 ctccggtccc tggtagcacg cttagccccc tcgagaagga gggctggcgt gaccatcagt
     2041 cagagcgcgg gatcccgctc acgtgcgccg gcgaagggcg gaacctgcac gccgacttcc
     2101 gttgtgtgtc accgacgtac gcgtcgggcg gcacgcgcga ctggcccgca cgcgtgtcaa
     2161 cgcggtgtcg tccgggcgcg gcgcccttca ccgtctgcac agcgacgcgt gtttgcacga
     2221 gcacctttga ctgtgctctc tgggcgtcgg gcctgttgga gttccactgc gtcattggtc
     2281 ggcaacgagg tcgttctccg
//
LOCUS       DASUGA010000056         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1512676, whole genome shotgun sequence.
ACCESSION   DASUGA010000056 DASUGA010000000
VERSION     DASUGA010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512676"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VFX21_01050"
     CDS             complement(<1..365)
                     /locus_tag="VFX21_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017197740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5094562.1"
                     /translation="MDDVFRALADPTRRSLLDELFREDGQTLHALEAKFEMTRFGVMK
                     HLKVLEDAGLVVTKKRGREKLHFLNAVPIRLIHDRWVSKYAEPWAAALSGLKTDLERP
                     MEKVFEIFIKTTPETLWQA"
     gene            458..1048
                     /locus_tag="VFX21_01055"
     CDS             458..1048
                     /locus_tag="VFX21_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5094563.1"
                     /translation="MTTQQVRRAQTRTRLLEAAAQLFATRGIDGSSIDAIADLADRTS
                     GAVYDHFGGKEGLLFALLESWVDDVAVVINAELAAATTLDERLATLWRNIVDPPSGGS
                     DWIALEHELWRYATRNDTARAQLARRYQAMWDGVGAAAGEWVEGASRVGPALIGLLLG
                     LEMMRRVDPDAVTDELAVAALRGVVEGAVLPKEKTR"
     gene            1045..2283
                     /locus_tag="VFX21_01060"
     CDS             1045..2283
                     /locus_tag="VFX21_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5094564.1"
                     /translation="MTATFESIDGIDLLATTWGSGVPHDQFDRLRAEAPVYFHEEPND
                     TGFWALTKYDDVKRASYEWETFSSELGGTFIPTADEEALATLRLTILNMDPPHHHRYR
                     RLVSRGFTPRMIQKLVDDINQRAVRVVDNVCEKGEVEFVEEIAGQVPVQMICEMIGLE
                     KELWPRMFEISNHLIGSRDDPDYQDRPETGDVAAAEIYMLCDAVAADRRANPRDDIMT
                     ALVQAEIDGEHLTDAELNLFFITLIVAGNETTRNLINHSLLALIEHPEAAQQLRDHPE
                     LWDSGVEEMLRWGSSIHNFRRTATKDTEIRGVPIAAGDKVVLYYASANRDEDVFENPH
                     TFDVTRSPNEHVTFGAGGVHYCLGASLARAEIKATMRQIVERLPDIELTAEPNRLHSD
                     FVNGIKTMPVRFTPSKPVGA"
     gene            complement(2288..>2566)
                     /locus_tag="VFX21_01065"
     CDS             complement(2288..>2566)
                     /locus_tag="VFX21_01065"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5094565.1"
                     /translation="GFSRGQLRRASATQGIAMGLLAALVGVPLGLLVGKWVWTTHADR
                     IGLSTAITLPIGVGLAIAAVTVVLTCLIAVAAGWRATRGRLATALRTE"
BASE COUNT          474 a          833 c          837 g          422 t
ORIGIN      
        1 atcgcctgcc aaagagtttc gggcgtggtc ttgatgaaga tctcgaacac cttttccatg
       61 ggtctctcca ggtcggtctt gagtccgctg agcgcggcgg cccagggctc ggcgtacttg
      121 ctgacccacc ggtcgtgaat cagccggatc gggacggcgt tcaggaagtg gagcttctcc
      181 cggccgcgct ttttcgtaac cacgagccct gcgtcttcga gcaccttgag gtgcttcatc
      241 acgccgaagc gggtcatttc gaatttcgct tcgagggcgt gcagggtctg cccgtcttca
      301 cggaacagct cgtcgaggag gctccgccga gtcggatcgg ccagagcccg gaacacgtcg
      361 tccacggcgg tcgagaatag gtgactaatt agtcacctgt caatcggtgt ttggataggc
      421 ggtccgagat atctcggacc gcctatatat ttgtcccgtg acgacgcagc aggtgcggcg
      481 ggcgcagacc cgtacccggc tcctcgaggc ggccgcgcag ttgttcgcga cgcgcgggat
      541 cgacggttcg agcatcgacg cgatcgcgga tctcgccgac cgcacctcgg gcgccgtcta
      601 cgaccacttc ggcggcaaag aaggcctgct ctttgcgctg ctcgaaagct gggtcgacga
      661 cgtcgcagtg gtcatcaacg ccgaactcgc cgccgcgacg acgctcgacg aacggctcgc
      721 cacgttgtgg cgcaacatcg tcgaccctcc gagcggcggt agcgactgga tcgcgctcga
      781 acacgagctc tggcgctacg ccacgcgcaa tgacactgca cgcgcccaac tggcgcgccg
      841 ataccaagcg atgtgggacg gcgtcggtgc ggcggcgggc gagtgggtcg agggcgcgtc
      901 gcgcgtcggc ccggcgttga tcggattgtt gctgggactg gaaatgatgc gccgcgtcga
      961 ccccgacgcg gtgaccgacg aactggccgt ggccgccctc cgaggagtgg tcgagggcgc
     1021 ggtgctaccc aaggagaaaa cgcgatgact gcgacgttcg agtcgatcga cggcatcgac
     1081 ctgctcgcca caacgtgggg cagcggggtt ccgcacgacc agttcgaccg gttgcgggcc
     1141 gaggcgccgg tctacttcca cgaagagccg aacgacaccg gcttttgggc gctcacgaag
     1201 tacgacgacg taaagcgcgc cagctacgaa tgggagacgt tctcctcgga gctcggcggc
     1261 acgttcatcc cgactgccga cgaggaagca ctcgcaacgt tgcggctgac gatcctcaac
     1321 atggatccgc cgcaccacca ccgctaccgc cgtctcgtct cgcgcggttt tacgccgcgc
     1381 atgatccaga agctcgtcga cgacatcaac cagcgcgcgg tgcgcgttgt cgacaacgtg
     1441 tgcgagaagg gcgaggtcga gttcgtcgag gagatcgcag gccaggttcc ggtccagatg
     1501 atctgcgaga tgatcggcct cgagaaggaa ctctggccgc gcatgttcga gatctcgaac
     1561 cacttgatcg gttcgcgtga cgatcccgac taccaggacc ggcccgagac cggcgacgtc
     1621 gccgcggccg agatctacat gctgtgcgac gcggtcgctg ccgaccgccg cgccaatccg
     1681 cgcgacgaca tcatgacggc gctcgtgcag gccgagatcg acggtgagca cctgaccgac
     1741 gccgagctca acctgttctt catcacgctc atcgtcgcgg gcaacgagac gacgcgaaac
     1801 ttaatcaacc actcgctgtt ggcgttgatc gagcaccccg aggccgcgca gcaactgcgc
     1861 gaccatcccg aactgtggga cagcggcgtc gaggaaatgc tgcgctgggg aagttcgatc
     1921 cacaacttcc ggcgtacggc aacaaaggac accgagattc gtggcgtgcc gattgcggcc
     1981 ggcgacaagg tcgtcctcta ctacgcgtcc gcgaaccgtg acgaggacgt gttcgagaac
     2041 ccgcacacgt tcgacgtgac ccggtcgccc aacgaacacg tgacgttcgg ggccggcggt
     2101 gtgcactact gcctgggcgc gagcctggca cgggccgaga tcaaagcgac gatgcgtcag
     2161 atcgtcgagc ggctgcccga catcgagctg accgcggagc cgaaccggtt gcactccgac
     2221 ttcgtgaacg gcatcaagac gatgccggtg cgcttcacgc cgtcgaagcc ggtcggcgcc
     2281 taagtcgcta ctccgttcgc aacgcggtcg ccaggcggcc gcgagttgcg cgccaaccgg
     2341 ccgcgaccgc gatgaggcac gtcaacacga ccgtcactgc tgcgatcgcg agcccgacgc
     2401 cgatcggaag cgtgatcgcg gtgctgaggc cgatccgatc ggcatgggtg gtccagaccc
     2461 atttgccaac gagcaaaccg agtggcaccc cgacgagcgc ggcgagcaga cccattgcaa
     2521 tgccttgcgt ggctgatgcg cgccgaagct gcccccgact gaatcc
//
LOCUS       DASUGA010000057         2249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_680223, whole genome shotgun sequence.
ACCESSION   DASUGA010000057 DASUGA010000000
VERSION     DASUGA010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680223"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VFX21_01070"
     CDS             complement(<1..532)
                     /locus_tag="VFX21_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013426437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5094566.1"
                     /translation="MPEQPDIDIPTSFAAGADVNGKRVVITGASRGLGRLLAHAFSHA
                     SARVTLVARTEKDLKAVADELPGPSVVCGGDVTDAEFNEAVADATVAEWDGVDVWICN
                     AGISPVLAGPRQTDPSVWRQILDVNLTGAFLGARAAARVMQDGGRVIFTGSVLGERPR
                     AGLAAYSASKAGLVGLA"
     gene            576..>2249
                     /locus_tag="VFX21_01075"
     CDS             576..>2249
                     /locus_tag="VFX21_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5094567.1"
                     /translation="MFTDAEVLEMQRRMLVIRGFEERVATLYRDGEVPGFVHLSIGQE
                     AAAVGACWPLGPRDVITSTHRGHGHCLAKGLDPRGMFAELMGKDAGTNRGRGGSMHIA
                     DPALGIFGANGIVGAGLPIAAGAATAAQLRGDGGIAVAFFGDGAVAHGTFHEAVNLAA
                     VWQLPVVFFCENNGYAEFSPASTQHSATLEQRAAGYGVDYVAVDGNDVLAVADAMRRV
                     VEGVRQGKGPVLVEATTYRRHGHYEGDPQRYRTPEEVTEWDARDPLVVHERRLREAGI
                     SDDWLAALQSSVARELDDAVAAARPLPAPAVGTLHDFVVRPHPERSEPPALAPDLDVF
                     RTMDAIRAALETELANDERVFVAGIDVAAGGNVFGLTRGLHDRFGTRVRDTPISESAI
                     VGVGVGAAMAGMRPVVELMYLDFLGVCLDQLLNQAAKMPYMTGGAAEMALTVRTQFGA
                     GRSSGSQHSQSLEALLAHIPGLTVVMPSTPADTYGLLRAAIQDPNPVVFVENRLLYGM
                     KGPQPPSEHIVPIGRAAVVRPGDDITVVSVSRMVHEALAAADAVAPDGIS"
BASE COUNT          325 a          803 c          788 g          333 t
ORIGIN      
        1 tggcgagacc gacgagaccg gccttcgacg cgctgtacgc ggcgagtccg gcacgcggcc
       61 gttcgccgag caccgatccg gtgaagatca cccgtccgcc gtcctgcatc acgcgcgccg
      121 cggcgcgcgc accgaggaac gcgcccgtga ggttcacgtc gaggatctgg cgccacacac
      181 tcgggtcggt ctggcgcggt cccgccaaca ccggcgagat gccggcgttg cagatccaga
      241 cgtcgacgcc atcccactcg gcaactgtcg cgtcggcgac tgcttcgttg aactcggcgt
      301 cggtgacgtc accgccgcac acgaccgacg gaccgggcaa ctcgtcggcg acggccttca
      361 agtccttctc ggtgcgcgcc accaacgtca cgcgcgcgct cgcatgcgag aacgcgtgcg
      421 cgagcaagcg tccgaggccg cggctcgcgc cggtgatcac gacgcgcttg ccgttcacgt
      481 ccgcaccggc ggcgaaggac gtcgggatgt cgatgtcggg ctgctcgggc atcgccccgt
      541 ccctcgtttg gactgatcgt tacaatacac actccatgtt caccgacgcc gaggtgctcg
      601 agatgcaacg gcgcatgctc gtgatccgcg gcttcgagga gcgcgtcgcg acgctctacc
      661 gcgacggtga agtgcccggc ttcgtgcacc tgtcgatcgg ccaggaagcc gcggccgtcg
      721 gcgcgtgctg gccgctcggt ccgcgcgatg tcattacgtc gactcatcgc ggccacggcc
      781 attgcctcgc gaaaggtctc gatccgcgcg gcatgttcgc ggagttgatg ggcaaggacg
      841 cgggcaccaa ccgcggccgc ggcgggtcga tgcacatcgc cgatccggcg ctcggcatct
      901 tcggcgcgaa cggcatcgtc ggcgccgggc tcccgatcgc ggcgggtgcg gcaacggccg
      961 cgcaactgcg cggtgacggc ggcatcgcgg tcgcgttctt cggcgacggc gcggtcgcgc
     1021 acggcacgtt ccacgaagcc gtcaacctcg ccgcggtgtg gcagctcccc gtcgtgttct
     1081 tctgcgagaa caacgggtac gccgagttct cgcccgcgtc gacgcaacac tcggcgacac
     1141 tcgaacagcg cgcagccggc tacggcgtcg actacgtcgc ggtcgacggc aacgacgtgc
     1201 tcgcggtggc cgacgcgatg cggcgggtcg tcgagggcgt gcggcagggc aaaggcccgg
     1261 tgctggtcga ggcgacgacg taccgccggc acgggcacta cgaaggcgac ccgcagcgct
     1321 accgcacgcc ggaggaagtc accgagtggg acgcgcgcga cccactcgtc gtccacgagc
     1381 gcaggctccg ggaggcgggt atctccgacg attggttggc cgcgttgcag tcgtcggtgg
     1441 cccgtgagct ggacgacgcg gtcgccgcgg cgcgcccgct gcccgcgccc gcggtcggga
     1501 cgctgcacga cttcgtcgtc cgcccgcatc ccgaacgatc ggagccgccc gcgctcgcgc
     1561 ccgatctcga cgtgttccgc acgatggacg cgatccgcgc cgccctcgaa accgagctcg
     1621 cgaacgacga gcgggtcttc gtcgccggga tcgacgtcgc cgcgggtggc aatgtgttcg
     1681 ggctgacgcg cggtctgcac gatcgcttcg gcacgcgcgt gcgcgacacg ccgatctccg
     1741 agagcgcgat cgtcggcgtc ggcgtcggcg ccgcgatggc ggggatgcgg ccggtggtcg
     1801 agctcatgta cctcgacttc ctcggcgtgt gcctcgacca gttgctgaac caagcggcga
     1861 agatgccgta catgaccggc ggcgccgcgg agatggcgct cacggtgcgc acgcagttcg
     1921 gcgccggccg atcgtcgggg agccagcact cgcagagcct cgaagcgctg ctcgcgcaca
     1981 ttcccggctt gacggtcgtc atgccgtcga cgccggccga cacgtacgga ttgctgcgcg
     2041 ccgcgatcca ggatccgaac ccggtcgtct tcgtcgagaa ccgcttgctg tacgggatga
     2101 agggcccgca accaccgtcc gagcacatcg tgccgatcgg gcgcgccgcc gtcgtgcgcc
     2161 ccggtgacga catcaccgtc gtgtcggtgt cgcgtatggt gcacgaagcg ctcgccgccg
     2221 cggacgcggt cgcacccgac ggcatctcc
//
LOCUS       DASUGA010000058         2071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1375236, whole genome shotgun sequence.
ACCESSION   DASUGA010000058 DASUGA010000000
VERSION     DASUGA010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375236"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2071)
                     /locus_tag="VFX21_01080"
     CDS             complement(<1..>2071)
                     /locus_tag="VFX21_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018100816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEX5094568.1"
                     /translation="WIDDVREAFVAARGEEAGLDLVRAWGDAFPVAYRENFDGQDALA
                     DLALLEHLDDANPLAVRLVAVDGRYLDLKLYGIGTQPSLSTVLPHLANLGVLVDDEQP
                     YDVRPAGMSARWIKHFRMRVPEHSPAAGSVHRLFEDAFLAVLRGDMECDGLNRLVLTA
                     GLSAREISLLRTYSRYLRQIGTPYSQEYIADALVAHPDIARTLVELFVARLDPWSQGQ
                     GQDTDGLAEECTIALDAVSSLDEDRILRALLHLVRATLRTNWFQTDGANASRPCIVLK
                     LDPSQIPDLPLPRPMFEIFLSSPRVEGVHLRAGKVARGGIRWSDRREDFRTEILGLMK
                     AQRVKNAVIVPSGAKGGFVVKQPPAREQLAAEVEACYRIFIASLLDVTDNLVDGAVVP
                     PPAVVRYDGDDPYLVVAADKGTASFSDIANEIALARGHWLGDAFASGGSDGYDHKKMG
                     ITARGAWESVKRHFREFGVDTQSTDFTVAGIGDMSGDVFGNGMLLSEHIRLVAAFDHR
                     HVFLDPNPDAASSFAERKRLFDVPRSSWDDYDRAVISEGGGVYARTLKAIPISAAARD
                     ALGMPAGIETLTPAELIRAILRAPVELLWNGGIGTYVKARAETNLDVGDKANDSVRVD
                     GADLRCRVVGEGGNLGFTQRGRVEFALAGGMINTDAIDNSAGVDTSDHEVNIKILLDR
                     IVRSGALT"
BASE COUNT          318 a          701 c          742 g          310 t
ORIGIN      
        1 gtcaacgcac cggaccgcac gatccggtcg agcagaatct tgatgttgac ctcgtgatcg
       61 gacgtgtcga cgcccgcact gttgtcgatc gcgtcggtgt tgatcatccc gcccgccagc
      121 gcgaactcga cgcgcccgcg ctgggtgaac ccgaggttgc cgccctcgcc gacgacccgg
      181 cagcgcaggt cggcgccgtc gacgcgcacc gagtcgttcg ccttgtcccc gacgtcgagg
      241 ttcgtctcgg cgcgggcctt gacgtaggtc ccgatgccgc cgttccacag cagttcgacc
      301 ggcgcgcgca ggatcgcgcg gatcagctcg gccggcgtga gcgtctcgat gccggcgggc
      361 atcccgagcg cgtcgcgcgc cgccgccgag atcgggatcg ctttgagcgt acgagcgtag
      421 acgccgccgc cttcggagat gacggcccgg tcgtagtcgt cccacgacga acgcggcacg
      481 tcgaagagtc gcttgcgctc cgcgaacgac gacgccgcgt cggggttcgg gtcgaggaag
      541 acgtggcggt ggtcgaacgc ggcaacgagg cggatgtgct cggacagcag catgccgttg
      601 ccgaagacat cgcccgacat gtcgccgatg ccggcgacgg tgaagtcggt cgactgggtg
      661 tcgacaccga actcgcggaa gtgacgcttc accgactccc acgcgccgcg ggcggtgatg
      721 cccatcttct tgtggtcgta gccgtcggag ccgcccgacg cgaacgcgtc gccgagccaa
      781 tgaccgcgtg cgagggcgat ctcgttggcg atgtcggaga acgacgcggt gcccttgtcc
      841 gccgcgacca cgaggtacgg atcgtcgccg tcgtagcgca cgaccgcagg cggcggcacg
      901 accgcaccgt cgacgaggtt gtcagtgacg tcgagcaggc tcgcgatgaa gatgcggtag
      961 cacgcctcga cctcggccgc gagctgttcc cgcgcgggcg gctgcttgac gacgaagccg
     1021 cccttggccc cgctggggac gatgaccgcg ttcttgaccc gttgggcctt catcaagccg
     1081 aggatctcgg tgcggaagtc ttcgcggcgg tccgaccagc ggatgccgcc gcgcgcgacc
     1141 ttgcccgcgc gcaggtgcac accctcgacg cgcggcgacg acaggaagat ctcgaacatc
     1201 gggcggggga gcggcaagtc ggggatctgc gacgggtcga gcttgagcac gatgcacggc
     1261 cggctcgcat tcgcgccgtc ggtctggaac cagttcgtcc gcagcgtcgc gcggacgagg
     1321 tgcaacagcg cgcgcaggat gcggtcctcg tcgaggctcg acaccgcgtc gagcgcgatc
     1381 gtgcactcct ccgcgagccc gtcggtgtcc tggccctgcc cttgcgacca cgggtcgagg
     1441 cgcgcgacga acagctcgac gagtgtgcgt gcgatgtcgg ggtgcgcgac gagcgcgtcg
     1501 gcgatgtact cctggctgta cggcgtgccg atctggcgga ggtaacggct gtacgtgcgc
     1561 agcaatgaga tctcacgagc cgacaagccc gcggtgagca cgagccggtt gaggccgtcg
     1621 cactccatgt cgccccgtag caccgcgagg aacgcgtctt cgaacagccg gtgcaccgac
     1681 ccggccgccg gcgaatgctc cgggacgcgc atgcggaagt gcttgatcca gcgggcgctc
     1741 atgccggccg gcctgacgtc gtagggctgc tcgtcgtcga cgaggacgcc caggttcgcg
     1801 aggtgcggca acaccgtcga cagcgacggt tgggtcccga tgccgtacag cttgaggtcg
     1861 aggtaccggc cgtcgacggc caccaggcgg accgcgagcg ggttggcgtc gtcgaggtgc
     1921 tcgagcagtg cgaggtccgc gagcgcgtcc tgaccgtcga agttctcgcg gtacgcgacg
     1981 gggaaggcgt cgccccacgc ccgcacgaga tcgagcccgg cttcctcgcc ccgcgccgcg
     2041 acgaacgcct cgcgaacgtc atcgatccag g
//
LOCUS       DASUGA010000059         2232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1512119, whole genome shotgun sequence.
ACCESSION   DASUGA010000059 DASUGA010000000
VERSION     DASUGA010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512119"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2232
                     /locus_tag="VFX21_01085"
     CDS             <1..>2232
                     /locus_tag="VFX21_01085"
                     /inference="COORDINATES: protein motif:HMM:NF017388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HEX5094569.1"
                     /translation="AHNGNQQVIDVYTVGFTTNASANDLLQRTADLGNGTFSTSNDAE
                     QLAVDIVDAINDIILKSQSFTSATVPSTRTAEGGQFYTSVFLPKTTEGYWEGHLKSWK
                     ITQAGSILDANGNCALNDPGAPTTCVAGSFLSTAVPFWDAGELLRVRTPASRNLITTV
                     LQAPGVSGVVPFERTSITAAHLNLSLAEIPNYPVAPYAVPTTVDALRDVVLYNLRGCE
                     LGSVGGACTERDWKLGDIFHSDPVVVAGPSAFQRDLDYRAFSNTYVHRKRVIVAGAND
                     GFMRIFDAGTWQTTPAPGRYDEGTGAEIVGFMPYATRQVAKGIAIDKGGRNVYGVDGS
                     PSLADVWFYSSPTANAAGDGTDWQSWHTVAVGGLRQGGNSYYALDVTNPAATGCTAPS
                     SGPGYPCYLWEFPREDAIGGTAAYMGQTWSQAVIVKIRVNPSVSTPGPTTPGYDRWVA
                     VFGTGYDDTSDPNSESTYNVLSTKGRAIVMVDLKTGRVIAEKKFDPTGSSSTTNPATF
                     AYSSTNPEQSMHYAFPASPGVFDLDFDGYADVIYMPDLGGNVWKWVIKGIGQDPINGT
                     GSVTQPNWPFRKMFAAPTYYAAGPPAKTYYKSMYFTPSATLKNGNLWLVLGTGERMNL
                     KFPGLAGTTDENNRLYTLVDSDALDKMSPTAVVTESDLALLPSDTGCADLSGYKGYYF
                     LGEEGEKFVTETDIFAYTVLVASYVPTVSANPCVTSGTAKLYAYKIYCGQGAFVDPGA
                     GGIS"
BASE COUNT          394 a          767 c          736 g          335 t
ORIGIN      
        1 ggcgcacaac gggaaccagc aggtgatcga cgtctacacc gtcggcttca cgacgaatgc
       61 gtccgccaac gacctgttgc agcgcaccgc ggacctcggc aacgggacct tctcgacgag
      121 caacgacgcc gagcagctcg cggtcgacat cgtcgacgcg atcaacgaca tcatcctcaa
      181 gtcccagtcg ttcacgtcgg cgaccgtgcc gtcgacccga accgcggaag gcggccagtt
      241 ctacacgagc gtcttcctgc cgaagacgac cgaaggctac tgggaaggcc acctgaagtc
      301 gtggaagatc acgcaggcgg gctcgatcct cgacgccaac ggcaactgcg cgctcaacga
      361 ccccggtgcg cccaccacgt gtgtggccgg atccttcctc tcgacggccg tcccgttctg
      421 ggacgcgggc gagctgctgc gcgtgcggac gccagcgtcg cggaacctga tcacgacggt
      481 gctccaggca cccggcgtga gcggtgtggt tccgttcgag cgcacgagca tcacggcggc
      541 gcatctcaac ctgtccctgg ccgagatccc gaactatccg gtggctccgt acgcggtgcc
      601 gacgaccgtg gacgcgctgc gcgacgtggt tctgtacaac ctgcgcggct gcgagctcgg
      661 ctcggtcggc ggggcctgca cggagcgcga ctggaagctc ggcgacatct tccactccga
      721 cccggtcgtc gtggcgggac cgtccgcgtt ccagcgcgat ctcgactacc gcgcgttctc
      781 gaacacctac gtgcaccgca agcgcgtgat cgtcgcgggc gcgaacgacg gcttcatgcg
      841 catcttcgac gccggcacct ggcagacgac gcccgcgccg ggccgctacg acgagggcac
      901 cggcgccgag atcgtcgggt tcatgcccta cgcgacgcgt caggtcgcga aggggatcgc
      961 gatcgacaag ggcgggcgga acgtgtacgg cgtcgacggc tcgccgagcc tcgcggacgt
     1021 gtggttctac tcgagcccga ccgcgaatgc ggccggcgac ggaaccgact ggcagagctg
     1081 gcacacggtc gccgtcggcg ggctgcgcca gggcggcaac tcgtactacg cgctcgacgt
     1141 gacgaacccg gccgcgacgg gctgcacggc gccgtcgagt ggccccggct acccctgcta
     1201 cctgtgggag ttcccgcgcg aggacgccat cggcggtacc gccgcctaca tgggccagac
     1261 ctggtcgcag gccgtgatcg tgaagatcag ggtgaacccg agcgtcagca cgccgggccc
     1321 caccactccg ggctacgacc gctgggtcgc cgtgttcgga accggctacg acgatacgag
     1381 cgatccgaac tccgagagca cctacaacgt gctctcgacc aagggccgcg cgatcgtgat
     1441 ggtcgatctg aagaccggcc gcgtgatcgc ggagaagaag ttcgatccca cgggctcgag
     1501 ctcgacgacg aaccccgcga cgttcgcgta ctcgtcgacg aaccccgagc agtcgatgca
     1561 ctacgcgttc ccggcttcgc cgggcgtgtt cgacctcgac ttcgacggct atgccgacgt
     1621 gatctacatg cccgacctcg gtggcaacgt gtggaagtgg gtcatcaagg gcatcggcca
     1681 ggacccgatc aacgggaccg gctcggtcac gcagccgaac tggccgttcc gcaagatgtt
     1741 cgcggcaccg acctactacg cggccggtcc gcccgccaag acctactaca agagcatgta
     1801 cttcacgccg agtgcgacgc tgaagaacgg caacctgtgg ttggtgctgg gaaccggcga
     1861 gcggatgaat ctcaagttcc cgggcctcgc gggaacgacc gacgagaaca accgcctcta
     1921 cacgctggtc gactcggacg cgctcgacaa gatgtcgccc acggcggtcg tcacggagag
     1981 cgatctggcg ctgctgccgt ccgacacggg ctgcgcggat ctctcgggct acaagggcta
     2041 ctacttcctc ggtgaggagg gggagaagtt cgtcaccgag acggacatct tcgcctacac
     2101 cgtgctggtg gcctcctacg tgccgacggt cagcgccaac ccgtgcgtca cgagcggtac
     2161 cgcgaagctc tacgcgtaca agatctactg cggccagggc gccttcgtgg acccgggcgc
     2221 gggtgggatc tc
//
LOCUS       DASUGA010000060         2040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_263915, whole genome shotgun sequence.
ACCESSION   DASUGA010000060 DASUGA010000000
VERSION     DASUGA010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263915"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1463
                     /locus_tag="VFX21_01090"
     CDS             <1..1463
                     /locus_tag="VFX21_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094570.1"
                     /translation="HDRSTGDPLPVVFTEHRVPVFNGLFAEVEHMFRDGNSLYLGVLA
                     GSPGCVVRLDLGTWLHQVVPGTGPGAVAGGGAMTMGNLSGGSGPDLVRFPIDGSIEVY
                     DTSGSVLRSGREGSSPTSAVTLDYNSLLSRTELCILDRDWWVRRYDAANGAYIDKFRM
                     PTPMTLPAKPSSMSLPKRMLRVATTTFGPSAFELFPHAGTMFPGNRVGLGTTTPVRVS
                     FAGAGLTASHSFTDEHDWLVTKSCYGEFEAAATGMKAWPISTNSPTATLLAPMTSQLF
                     ATGEAAVITVDLPNDGPNLVFSNEAGNVRVLSGFPSNPVMQAFQAASPWTGGIGPSQS
                     PAVVGDPGDTLLRLEALDVSSLATGELDGNPLTIDVVAGAMVPDAQGNTLFVLDVASG
                     SAVRVASAAVGSVSGVVLGDVDLDGFTDIVVGTVEGRLRVYDRNLNPILEVDTAAMMV
                     GARGTMWLIEGNAGQEVPSRLIFSHSEGVTAIDFAF"
     gene            complement(1501..>2040)
                     /locus_tag="VFX21_01095"
     CDS             complement(1501..>2040)
                     /locus_tag="VFX21_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094571.1"
                     /translation="KVVLRCRAGDHVVSVRVTGGGAQRAVVELPDWSEGRTALLVTVP
                     GGGELRGTIAPAQFVAALDCSTEDERKRASEFGAGHDPWLVSLGDLRAANRPVVALRT
                     RTNAERWDGGVAPLDENGAFAFAGVPPGDWDVVLVVRERNGNRVTERVVEPPLGAVTM
                     RAGETATVAYAAPEQVAGR"
BASE COUNT          277 a          738 c          705 g          320 t
ORIGIN      
        1 tgcacgatcg cagcaccggg gacccgctgc cggtcgtctt caccgagcat cgtgtgccgg
       61 tcttcaacgg cctgttcgcc gaggtcgagc acatgttccg cgacggcaac tccctctacc
      121 tcggcgtgct cgcgggttcg cccgggtgcg tcgtgcgcct cgatcttggc acctggttgc
      181 accaggtcgt gccgggcacc ggtcccggtg cggtcgccgg cggcggcgcg atgacgatgg
      241 gcaacctgag cggcggctcc ggccccgacc tcgtgcggtt cccgatcgac ggttcgatcg
      301 aggtctacga cacgtctggc tcggtgttgc gcagcggccg cgagggaagc tctccgacgt
      361 cggccgtgac gctcgactac aactcgttgc tgagccgcac agagctgtgc atcctcgacc
      421 gcgactggtg ggtgcggcgc tacgacgctg cgaacggcgc ctacatcgac aagttccgca
      481 tgccgacgcc gatgaccctg ccggcgaaac cgtcttcgat gtccctgccg aagcgcatgc
      541 tgcgcgtcgc gacgaccacg ttcggtccct cggccttcga gctcttcccg cacgccggca
      601 cgatgttccc ggggaaccgc gtcggactcg gcacgacgac gcccgtgcgc gtgtcctttg
      661 ccggagccgg gctgaccgcg tcgcactcgt tcaccgacga gcacgattgg ctggtgacga
      721 agtcgtgtta tggggagttc gaggccgcgg cgacggggat gaaggcctgg ccgatctcga
      781 cgaactcgcc gaccgcgacg ctgctggccc cgatgacgtc gcagctgttc gcgacgggcg
      841 aagccgccgt catcaccgtg gacttgccca acgacggccc gaacctcgtg ttctcgaacg
      901 aggcgggcaa cgtgcgcgtg ctgtccggtt tccccagcaa cccggtgatg caggccttcc
      961 aggcggcgag cccgtggacc ggcggcatcg gcccgagcca gagccccgcc gtggtcggcg
     1021 atccaggcga cacgctgctg cggctcgagg cgctcgacgt ctcgtcgctg gcgaccggcg
     1081 agctcgacgg caacccgttg accatcgacg tcgtcgcggg cgcgatggtc cccgatgccc
     1141 agggcaacac gctgttcgtg ctcgacgtcg ccagcggctc ggccgtgcgc gtcgcgagcg
     1201 ccgccgtcgg ctccgtcagc ggcgtcgtgc tcggcgacgt cgatctcgat ggattcacgg
     1261 acatcgtcgt cggcacggtc gaaggccgcc tgcgtgtcta cgaccgcaat ctgaacccga
     1321 tcctcgaggt ggacacggcc gcgatgatgg tcggcgcgcg cggcacgatg tggctcatcg
     1381 agggcaacgc cgggcaggag gtgccgagcc ggttgatctt ctcgcattcg gaaggtgtca
     1441 ccgcgatcga cttcgccttc tgagttgcgg gaagcacgcg cgcggtcgcc acgtttggtg
     1501 ctagcgaccc gcgacctgct cgggcgcggc gtacgcgacg gtcgccgtct caccggcgcg
     1561 catcgtcacg gcaccgagcg gcggctcgac cacgcgctcg gtgactcggt tgccgttgcg
     1621 ttcgcgcacc accagcacga cgtcccagtc gcccgggggc acgccggcga acgcgaacgc
     1681 gccgttctcg tcgagcggtg cgacgccacc gtcccaccgc tcggcgttcg tgcgcgtgcg
     1741 cagcgcgacg acgggccgat tcgccgcgcg caggtcgccg agcgagacca gccacgggtc
     1801 atggcccgcg ccgaactccg aggcgcgttt gcgttcgtcc tcggtgctgc agtcgagcgc
     1861 cgcgacgaac tgcgccggtg cgatcgtgcc gcgcagctcg ccgccgcccg ggaccgtcac
     1921 gagcagcgcg gtgcgccctt cgctccagtc cggcagctcg acgaccgcgc gctgcgcgcc
     1981 gccgccagtc acgcgcaccg acacgacgtg atcgccggcg cggcagcgca gcacgacctt
//
LOCUS       DASUGA010000061         4712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_122260, whole genome shotgun sequence.
ACCESSION   DASUGA010000061 DASUGA010000000
VERSION     DASUGA010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122260"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1569)
                     /locus_tag="VFX21_01100"
     CDS             complement(<1..1569)
                     /locus_tag="VFX21_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094572.1"
                     /translation="MRRAALLFALVVLGASFVVPAGTADSAGQMCTGRPPRTAAEFQS
                     VPDSRNATFGVGDLMSAVDLPDGRRLFVFGDTAYYDLQGASAGPLRAFGNNSAWVQSG
                     ACFTQLDNTREGNRSWVQPAEQDGTVYWPGASVVVGNRLHVFLAKIRPDNIFGTQVGA
                     AIATFELPSLELARIASVPWTPGRSYGSGAVYDGGYLYSFGSQRPNCDFCFAADMYVA
                     RVPEDQLQAPSTWQYYAGGGRWTTDPNGAGIVLPAATSSTNVTPYNNGFLLVTKPVSI
                     VGPDVDAWWAPDPVGPWTKLGRIFKMPEPPPSYIPGYHYNQSFTYTPTVVPSATLDDG
                     GILMGYNVLSFDPNEGVVDGRGFGPRFTSVTIPPTPAAGPRAAVAPSGSPWDAVVGVD
                     RAGRVYAAGGNVQYLTSHTNTAVGVARTITGRGSWAAAEDGGVFSIGDAQFYGSTGGM
                     RLNQPVVGIAATPTGRGYWLVAKDGGVFSFGDAQFYGSTGSMRLNKPILGIAATPSGR
                     GYWLVASDGGVFSFG"
     gene            complement(1635..3683)
                     /gene="ligA"
                     /locus_tag="VFX21_01105"
     CDS             complement(1635..3683)
                     /gene="ligA"
                     /locus_tag="VFX21_01105"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX5094573.1"
                     /translation="MAAKVPPDAIERVRSLRELIEYHTQRYFEADEPEISDAEFDDLV
                     RELRALEAAHPSLVTPDSPTQRPGGQAISSFAPVRHRARMFSLDNAFDHDELRAWATR
                     IEKLVPQKITYVAEPKLDGLAISLQYENGTFVRGATRGDGVTGEDVTENLRTIKAVPQ
                     KLKGNNVPALLEVRGEVFMPLAGFEQLNKRQEAAGDRLFVNARNAAAGSLRVKDVSVT
                     ASRDLTLFCYQTGVIDGGPKLKTHVETLAWLRKLGLPVNDKAKPIDNLDDVVAFCDDM
                     EAQRHSLGYDIDGVVVKVDDLGQRAEMGTTSRAPRWAIAYKFPPEERTTLLRDIMVSI
                     GRTGRATPFAVLEPVFVGGANVGMATLHNEDDVARKDVRPGDTVIVRRAGDVIPEVVG
                     PVAAKRPAKVKKQGPWKFPTKCPECKQPLVRVEGEANHHCVNVDCPMQRVQRLVHFAS
                     RGAMDIEGLGEERVRQFVDAGLLADAADIYDLTVEQLVPLERIGERSAQLLVTAIESS
                     KTQPLARLLVGLGINHVGPSAAQAFARELGHLDRIAAASEEELVAVEGVGTTIAQSIH
                     RFFEIDRNRTLIDRLRAAGCNFEGPAPAAVSEEEQTLAGLTFVLTGSLESRTRDEAAA
                     EIEARGGKVTGSVSKKTSYVVAGESPGSKLTKAEQLAVRILDEDAFERLLELGPDGEP
                     "
     gene            complement(3724..>4712)
                     /locus_tag="VFX21_01110"
     CDS             complement(3724..>4712)
                     /locus_tag="VFX21_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094574.1"
                     /translation="AGIATAIGSLPHSDAHAAAAAVLRCLPEFPAAPQLPNRCARENV
                     ISQWVHGIPGVESDSDGTLSIVGAVDEHEAVDTQFDRDVHGGLLAFLDATAALPRPPA
                     RVKAQVTGPLTLGVALLRAGVPAERAFPLGARAARSWARAVESLFAERLPGSALVLFF
                     DEPALVSWRDEDTAPVDREVATDLLSTALAAPGCTTGVHVCGAGDARLAMAAGPDVVH
                     FAVDEFRFDDAISLGRFIDGGGWVAWGAIPTHGPIGEHPSPLWKGLLDIWCELTRRGL
                     DPVRLRAQAVVAPACGLAGHGVSQAERAMLLARDLGNRVHDQAAATKLAVGA"
BASE COUNT          712 a         1602 c         1662 g          736 t
ORIGIN      
        1 gccgaacgag aacacaccgc cgtcgctcgc gaccaaccag tacccgcgcc cggacggcgt
       61 tgccgcgata ccgaggatcg gtttgttcaa ccgcatgctg cccgtagagc cgtagaactg
      121 cgcgtcgccg aacgagaaca cgccaccgtc cttggcgacc agccaatacc cgcggccggt
      181 cggtgtcgct gcgatgccga cgacgggttg gttgagccgc ataccgccgg tcgagccgta
      241 gaactgcgcg tcgccgatcg agaacacacc accgtcctcg gccgcggccc agctgccgcg
      301 gcccgtgatc gtgcgcgcca cgccgaccgc ggtgttggtg tgcgaggtga ggtactgcac
      361 gttgccgccc gcggcgtaga cgcgacccgc ccggtcgacg ccgacgactg catcccatgg
      421 tgagcccgac ggcgcgaccg cggcccgcgg gccggcggcg ggagtcggcg ggatcgtgac
      481 cgacgtgaag cgaggcccga acccgcgccc gtcgacgacg ccctcgttcg gatcgaacga
      541 cagcacgttg tatcccatga ggatgccgcc gtcgtcgagc gtcgcgctgg gcacgaccgt
      601 cggcgtgtag gtgaacgact ggttgtagtg gtaccccggg atgtacgacg gcggcggttc
      661 cggcatcttg aagatgcgcc cgagcttcgt ccacggaccg accgggtccg gcgcccacca
      721 tgcgtcgaca tcgggaccga cgatcgacac cggcttggtc acgagcagga agccgttgtt
      781 gtacggcgtg acattggtgc tactcgtcgc ggccggcagc acgatcccgg ccccgttcgg
      841 gtcggtcgtc caccggccgc cgcctgcgta gtactgccac gtgctcggcg cctgcagttg
      901 atcctcgggg acgcgcgcga catacatgtc ggccgcgaag cagaagtcgc agttgggccg
      961 ttgcgaaccg aacgagtaca ggtagccgcc gtcgtacacc gcacccgagc cgtaagaacg
     1021 gccgggcgtc catggaaccg acgcgatgcg cgcgagttcc agcgacggca actcgaacgt
     1081 ggcgatcgcg gcgccgacct gggtgccgaa gatgttgtcg ggacgtatct tggcgaggaa
     1141 cacgtggagg cggttgccga cgacgaccga cgcgccgggc caatagacgg tgccgtcctg
     1201 ctccgcaggc tggacccagc tgcggttgcc ttcgcgagtg ttgtcgagct gcgtgaagca
     1261 cgcaccggac tgcacccacg ccgagttgtt gccgaatgcg cgcagcggac ccgcgctcgc
     1321 gccctggagg tcgtagtagg cggtgtcacc gaagacgaac aaccggcgtc cgtcggggag
     1381 gtcgaccgcc gacatgaggt cgccgacacc gaaagtggcg ttgcgggagt ccgggacgct
     1441 ctggaactcg gcggccgtgc gcggcggccg gccggtacac atctgtcccg ccgaatccgc
     1501 ggtgccggcc ggcacgacga acgacgcgcc gagtacgacg agtgcaaaga ggagcgccgc
     1561 ccggcgcacg caggagatca tcggtgtctg agacgcccga acgtgtggat tcgttgcgaa
     1621 gcgagcgaag tctctcaggg ttcgccgtcg ggaccaagct ccaggagccg ttcgaatgcg
     1681 tcctcgtcga ggatcctgac agccaattgt tcggcctttg tgagctttga accagggctt
     1741 tcaccggcga cgacgtagct cgtcttcttc gagacactgc cggtgacctt gccgccacga
     1801 gcctcgatct cggccgcggc ttcgtcgcgg gtgcgcgact ccagcgatcc cgtcaagacg
     1861 aatgtcaggc cggcgagggt ttgctcctcc tccgagaccg cggccggggc cgggccctcg
     1921 aagttgcagc cggcggcgcg gagccggtcg atcaacgtgc ggttgcggtc gatctcgaag
     1981 aaccggtgga tgctctgcgc gatcgtcgta ccgacgcctt cgaccgccac gagctcctcc
     2041 tcgctcgcgg cggcgatgcg gtcgaggtgg ccgagctccc gcgcgaacgc ctgcgcggcc
     2101 gacggaccga cgtggttgat gccgaggccg acgaggagtc gcgcgagcgg ctgcgtcttc
     2161 gagctctcga tggcggtgac gagcagctgt gcgctgcgtt caccgatgcg ctcgagcggc
     2221 acgagctgct cgacggtgag gtcgtagatg tcggccgcgt cggcgaggag ccccgcatcg
     2281 acgaactggc gcacgcgctc ctcgccgagc ccttcgatgt ccatcgcgcc gcggctcgcg
     2341 aagtggacga gtcgctgcac ccgttgcatg ggacaatcga cgttgacgca gtgatggttc
     2401 gcttcgcctt cgacgcgaac gagcggctgt ttgcattcgg ggcacttcgt cggaaacttc
     2461 cacggtccct gcttcttcac cttcgcggga cgcttggccg ctaccggccc gacgacttcg
     2521 gggatgacgt cgccggcgcg gcgcacgatg acggtgtcgc cgggccggac gtctttgcgg
     2581 gcgacgtcgt cttcgttgtg gagcgtggcc ataccgacgt tggcgccgcc gacgaacacc
     2641 ggctcgagca ccgcgaacgg cgtcgcgcgt ccggtgcgcc cgatgctgac catgatgtct
     2701 ctcaggagcg tcgtgcgctc ctcgggcggg aacttgtacg cgattgccca gcgcggcgcg
     2761 cgcgacgtcg tgcccatctc cgcgcgctgg ccgagatcgt cgaccttgac gaccacgccg
     2821 tcgatgtcgt aaccgagcga gtgccgctgg gcctccatgt cgtcgcagaa cgcgacgacg
     2881 tcgtcgaggt tgtcgatggg cttggccttg tcgttgacgg gaaggccgag cttgcgcagc
     2941 cacgcgagcg tctcgacgtg cgtcttgagc ttcggaccgc cgtcgatgac acccgtctgg
     3001 tagcagaaca gcgtgaggtc gcggctggcg gtgacgctga cgtctttcac gcgcaggctg
     3061 cccgccgccg cgttgcgggc gttgacgaac aggcgatcgc cggcggcttc ctgtcgcttg
     3121 ttcagctgct cgaaccccgc gagcggcatg aacacctcac cccgcacttc gagcagcgcc
     3181 ggcacgttgt tgcccttcag cttctgcggg acggccttga tcgtgcggag gttctcggtg
     3241 acgtcttcac cggtgacgcc gtcgccgcgg gtcgcgccgc gcacgaaggt gccgttttcg
     3301 tactgcaacg agatcgcgag cccgtcgagc ttcggctccg cgacgtacgt gatcttctgc
     3361 gggacgagct tttcgatgcg cgtcgcccac gcgcgcagct cgtcgtggtc gaacgcgttg
     3421 tcgagcgaga acatccgggc ccggtgcctg accggtgcga acgacgagat cgcctgcccg
     3481 cccggccgtt gcgtgggtga gtcgggcgtg accagcgacg ggtgcgcggc ttcgagcgcg
     3541 cgcagctcgc gcaccagatc gtcgaactcg gcgtcggaga tctcgggttc gtcggcttcg
     3601 aagtaccgct gcgtgtggta ctcgatcagc tcgcgcagcg accgcactcg ctcgatcgcg
     3661 tcggggggca ccttcgcggc cacggccgca gactattgag cgggtcggac gacgaagtgc
     3721 cgactaggcg ccgacggcga gcttggtggc cgcggcctgg tcatgcacgc ggttgccgag
     3781 gtcgcgcgcg agcagcatgg cgcgctcggc ctgactcact ccgtggcccg ccagcccgca
     3841 ggccggcgcg acgaccgcct gggcgcgcag ccgcacggga tcgaggccgc gccgcgtcag
     3901 ctcgcaccag atgtcgagca ggcccttcca caacggtgac gggtgctcac cgatgggtcc
     3961 gtgcgtcggg atcgcacccc acgcgaccca gccgccgccg tcgatgaagc gccccagcga
     4021 gatcgcgtcg tcgaagcgga actcgtcgac cgcgaagtgc acgacgtcgg ggccggcggc
     4081 catcgcaaga cgcgcgtcgc ccgcgccgca cacgtgcaca cccgtggtgc atcccggcgc
     4141 cgcgagcgcg gtcgacaaca agtcggtggc gacttcgcgg tcgaccggcg cggtgtcttc
     4201 gtcgcgccag ctcacgagcg cgggttcgtc gaagaagagg acgagcgcgc tgccgggcag
     4261 tcgctcggcg aacaacgact cgacggcgcg ggcccagctc cgggcggcgc gtgcgccgag
     4321 cgggaacgcg cgctcggcgg gcacacccgc gcgcaacagc gcgacaccga gcgtcagcgg
     4381 cccggtgacc tgcgccttga cgcgcgccgg cggacgcggc agcgccgcgg tcgcgtcgag
     4441 gaacgcgagc aagccgccgt gcacgtcgcg gtcgaattgc gtgtcgacgg cttcgtgttc
     4501 gtcgaccgcg ccgacgatgc tgagcgtgcc gtcgctatcg ctctcgacac ccgggatgcc
     4561 gtgcacccat tgcgagatga cgttctcgcg cgcgcagcga ttcgggagtt gcggcgccgc
     4621 cgggaactcg ggcaggcaac gcaacactgc ggccgcggcc gcatgcgcat cggagtgcgg
     4681 aagactccca attgcggtcg caattcctgc tg
//
LOCUS       DASUGA010000062         3077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1791375, whole genome shotgun sequence.
ACCESSION   DASUGA010000062 DASUGA010000000
VERSION     DASUGA010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791375"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VFX21_01115"
     CDS             <1..771
                     /locus_tag="VFX21_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006538124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5094575.1"
                     /translation="VGEVVEPTRSVSTEQIGTAAPLGRPPLVVDLSSLWAGPLCAHVL
                     GRHGARVVKVESRTRPDGARAGPADFFDWLHGGHDSVVLDLTEPDGRQRLRQLLLAAD
                     VVIEASRPRALQQLGVVARDIVRDGPRVWISITGYGRQAAPLAVAFGDDAAAAGGLVA
                     WGEDGVPRFAVDAIADPLSGLAAAGAAHRALDRGGRWMIDVAMAGVAAAVVGADAGRT
                     WDAVPSNDAAQPRAEPPRATAHALGADTDAVFAELGLA"
     gene            805..2214
                     /locus_tag="VFX21_01120"
     CDS             805..2214
                     /locus_tag="VFX21_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018505706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5094576.1"
                     /translation="MRVLIRRAEVDGEVVDVRIDGERITHVRPSLRPHADDVVVDARG
                     GALLPGLHDHHLHVLALAAAERSVSVGQPDVVDHASFVETLRRAHRDLPLDRWIRAVG
                     YHERVAGDLDRHALDAIVRDRPTRVLHRGGALWTLNGAGLAAIGLGPTRSTDVPEGVE
                     LDADGCPTGRLWRLDTWLGERVPREPLDLRSTSRRLLRCGVTGLTDATPTDDPGPVQA
                     LARAMESRDIAQRLMVTGGLALPRDTNAHVELGPVKLLFPDHRPVSLDEMVAGIRVAR
                     EQGRTIAVHAVTSATVALVVAALDEVGARPGDRIEHGAVIPIGLVRRLADHGLTVVTN
                     PGFVASRGDDYLADVEADEQPDLWRCRSLLDAGVAVAAGTDAPYGDPNPWTAIDAATR
                     RRTASGAPLKPDESVPPSTALDMFLSSPDDPGGARRVVRAGASSDLCLLPVPLREALD
                     DPAAVQPSAVVVRGELIVW"
     gene            complement(2227..>3077)
                     /locus_tag="VFX21_01125"
     CDS             complement(2227..>3077)
                     /locus_tag="VFX21_01125"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEX5094577.1"
                     /translation="VARALREGQAIGDLQPEHLIASGQSQSAAALVSYINGVQPMTQA
                     FDGFFVHSRGGAGLPFPAVGESVDIASAIGRPGSILRTDTSVPVLDLQAENDLVGVLG
                     SLEARQPDSDTFRLWEVAGTAHADRTLVGEGTADLIDCGVPINGAPMYLVVRAAMHQL
                     VDWVVTGEPPPEAARLEVTEGDDPAIRRNADGIALGGVRTPPVDVPIEVFSGESGPGG
                     EAICILSGSTTPLPPERIAALYPSREAYEQAYRDAVGDVIEAGFLLEADRAEIEGYAR
                     PELVAG"
BASE COUNT          420 a         1135 c         1068 g          454 t
ORIGIN      
        1 gtgggtgaag tggtcgagcc aacccgctcg gtcagcaccg agcagatcgg taccgcggcg
       61 ccgctcgggc gaccgcccct cgtcgtggat ctctcgtccc tctgggccgg accgttgtgc
      121 gcgcacgtgc tcggtcgcca cggcgcgcgc gtggtgaagg tggaatcgcg aacccgtccc
      181 gatggcgcgc gcgccggtcc cgccgacttc ttcgactggc tgcacggcgg acacgactcg
      241 gtcgtgctcg atctcacgga acccgacgga cgccaacggt tgcgccaact gctcctcgcc
      301 gccgacgtgg tgatcgaggc ctcccgccca agggcgctcc aacagctcgg cgtcgtggcg
      361 cgcgacatcg tgcgcgacgg tccgcgggtc tggatctcga tcaccggcta cggacgacag
      421 gcggcgccgc tcgcggtggc gttcggagac gacgcggcgg cggcaggtgg tctcgtcgcg
      481 tggggcgagg atggcgtgcc gcgcttcgcg gtggacgcca tcgccgaccc gctctcgggg
      541 ctggcggcgg ccggcgcggc acaccgcgcc ctcgaccgcg gtggccgatg gatgatcgac
      601 gtcgccatgg cgggcgtcgc cgccgcggtc gtgggcgccg acgcgggccg aacctgggat
      661 gccgtcccga gcaacgacgc ggcccagcca cgagccgagc caccgcgagc aaccgcgcac
      721 gcgctcggcg ccgacaccga tgcggtgttc gcggagctcg ggctcgcctg agcgtcgcac
      781 caccccgccg accatgctgg ggccgtgcgc gtgctgatcc gacgagccga ggtcgacggc
      841 gaggtcgtcg acgtgcgcat cgacggcgag cggatcaccc acgtccgccc ttcgttgcgc
      901 ccgcacgccg acgacgtcgt ggtcgacgcg cgtggtggcg cgctcctccc cggtctgcac
      961 gaccatcacc ttcacgtcct cgcgctggcg gccgcggaac gttccgtctc ggtcggccag
     1021 ccggacgtcg tcgaccacgc ctccttcgtc gaaaccctgc ggcgcgccca tcgcgacctg
     1081 cccctcgacc gatggatccg tgccgtcgga taccacgagc gcgtggccgg tgatctcgac
     1141 cggcacgcgc tcgacgcgat cgtgcgcgat cgacccacgc gggtgctgca tcgcggcggt
     1201 gcgctctgga cactgaacgg cgcgggcctc gccgccatcg gcctcggtcc aacccgatct
     1261 accgacgtgc ccgagggtgt cgagctggac gccgacggat gccccaccgg acgcctgtgg
     1321 cggctcgaca catggctcgg cgagcgcgtc ccgcgcgaac cgttggacct gcgcagcacg
     1381 agccggcgcc tgctgcgttg cggcgtgacc ggcctcaccg acgccacgcc caccgacgac
     1441 cccggcccgg tgcaggcgct cgcgcgtgca atggagtcgc gagacatcgc gcaacgactc
     1501 atggtcacgg gcggtctcgc gttgcctcgc gacacgaacg cacacgtcga gctcggtccg
     1561 gtgaagctgc tgttccccga tcaccgaccc gtgtcactcg acgagatggt cgccggcatt
     1621 cgcgtcgcac gcgagcaggg acgcaccatc gcggtgcacg cggtcacgtc ggccaccgtt
     1681 gccctcgtgg ttgcggcgct cgacgaagtc ggtgcgcgac cgggtgatcg catcgagcat
     1741 ggtgccgtca tccccatcgg actggtacgg cggttggccg accacggcct gaccgtcgtg
     1801 accaatccag gtttcgtcgc gtcacgcggt gacgactacc tcgccgacgt cgaggccgac
     1861 gagcaacccg acctctggcg ttgccgctcg ttgctggacg caggtgtggc cgtcgcggca
     1921 gggaccgacg cgccctacgg cgatcccaac ccgtggacgg ccatcgatgc cgcgactcgc
     1981 cgccgaaccg cgagcggtgc gccgttgaaa cccgacgagt cggtgccgcc gtcgaccgcg
     2041 ttggacatgt tcctctcgtc ccccgacgat cccggtggcg cgcgccgcgt cgtgcgcgcc
     2101 ggcgcgtcga gcgacctttg cctactgccc gtgccgttgc gcgaagcgct ggacgatccg
     2161 gccgcggtgc aaccatccgc cgtggtcgtg cgcggcgagc tcatcgtctg gtgagcccga
     2221 tcctcctcag ccagccacga gttcgggacg ggcgtagccc tcgatctcgg ctcgatccgc
     2281 ctcgaggagg aagccggctt cgatgacgtc gccgacagcg tcgcgatagg cctgctcgta
     2341 cgcctccctc gacgggtaga gcgccgcgat ccgctcgggt gggagcggcg tggtggaccc
     2401 ggagaggatg cagatcgcct cgccaccagg ccccgattca ccgctgaaca cctcgatggg
     2461 cacgtcgacc ggcggtgtgc ggacgccgcc cagcgcgatg ccgtccgcgt tccgtcgaat
     2521 cgcggggtcg tccccttcgg tcacttcgag ccgagccgcc tccggcggcg gttcaccggt
     2581 gacgacccag tcgaccaact ggtgcatggc ggcccgcacg accaggtaca tgggagcgcc
     2641 gttgatgggc acgccgcagt cgatgagatc agctgtgccc tcacccacca aggtgcgatc
     2701 ggcgtgcgca gtgcccgcga cctcccacag ccggaacgtg tcgctgtcgg gttgccgcgc
     2761 ttcgagcgag ccgagcacgc cgacgagatc gttctcggcc tgcaggtcga gcaccggcac
     2821 gctcgtgtcg gtgcgcagga tgctgcccgg tcgtccgatc gcgctcgcga tgtcgaccga
     2881 ctcgcccaca gcggggaacg gcaggcccgc cccgccccgg ctgtgcacga agaagccgtc
     2941 gaacgcctgg gtcatcggct gcacgccgtt gatgtagctg acgagggccg cggccgactg
     3001 cgattgaccc gacgcgatga ggtgctccgg ctggagatca cctatcgctt gaccctcgcg
     3061 aagcgctcgc gcgacct
//
LOCUS       DASUGA010000063         2910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1928198, whole genome shotgun sequence.
ACCESSION   DASUGA010000063 DASUGA010000000
VERSION     DASUGA010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1928198"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VFX21_01130"
     CDS             <1..492
                     /locus_tag="VFX21_01130"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HEX5094578.1"
                     /translation="DGEPGELLIAGPTVMQGYWARDSETAQRLVTLTLDGHDVVAYRT
                     GDLVAAEPDGNYRFLGRRDNQIKSRGFRIELGEIETAIHAHDDVLEGAVVAVPDELVT
                     NRIRAFVVLREGTTSRDVAAFVADRLPKYMVPEQFSELDVLPRTSTGKIDRQALAGIA
                     SED"
     gene            510..1652
                     /locus_tag="VFX21_01135"
     CDS             510..1652
                     /locus_tag="VFX21_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014669213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5094579.1"
                     /translation="MDFAWTEEQEAYRDVVVKFASALPDDVRSRDGDGEFAWDAWKRC
                     AAFGIQGLNVPEEYGGADADALTTIVAMEALGYGSRDNGLLFSINAQMWACQHPIVQF
                     GTEEQKRKYLPRLCDGSSIAAHGMSEPGSGSDAFALATTAVLDGDHYILNGSKIFVTN
                     APVADLFVVFATTNPGKGFAGLCGFLVERDTPGFTVGRPLEKMGLRTSPMAELFFDDA
                     RVPVANLIGKPRGGMAIFNAAMERERSLILACTIGTMERLVEDSLAYAKQRRQFGQRI
                     GKFQAVSHRIVEMKLRLETARLLLYRLGWLIDQGRPIGLDAALTKLYVSECFVQSGLD
                     AVHVFGGYGYMQEYDVERVVRDALGSRLYSGTSDIQRNLAAMRLGL"
     gene            1683..2081
                     /locus_tag="VFX21_01140"
     CDS             1683..2081
                     /locus_tag="VFX21_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094580.1"
                     /translation="MVFAWPHRAIARAGQPQFVTLRENATGRIQTGVRDVCQFGRAGS
                     RTAIVPERHVTIDQGGVGLRIEESRVARELRRLGMPELAVERAVASGLGLTPAEVTEF
                     TARPFAADDRSDEIAVVESQIRSLRDQLGI"
     gene            complement(2157..>2910)
                     /locus_tag="VFX21_01145"
     CDS             complement(2157..>2910)
                     /locus_tag="VFX21_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEX5094581.1"
                     /translation="KDACVAVRAAVEDPEERVKRQQRSRSFRMWTDTDGMMVGRFAVT
                     PEVGGQIKSIVDQGVQQIFRSRRAGEEHEPHQAYAADVFANAFLRPAEVPAAKVTTHL
                     VMDHSILAVGDQLPGAKCEIPGVGPVNAQWARDLLGESFLTFVIKKGKDITTVAHFGR
                     HIPAELRTALVVSGRECDVEGCHNRGYLEIDHSHPVAKGGLTSWANLRWLCYLHHKRK
                     TQGYELPRKRSQPATESRARRPVPVGARSPDP"
BASE COUNT          479 a          950 c          993 g          488 t
ORIGIN      
        1 gacggcgagc caggggagct gctgatcgcg ggtccgacgg tgatgcaggg atactgggcg
       61 cgcgactcgg agactgcgca acgcctcgtg acgctcactc tcgacggtca cgacgtcgtc
      121 gcctaccgca ccggcgacct ggtggccgcc gagccggacg gcaactatcg cttcctcggc
      181 cggcgcgaca accagatcaa gagccgcggg ttccgcatcg agctgggcga gatcgagact
      241 gcgatccacg cccatgacga cgtgctggag ggcgcggtgg tcgcggtgcc ggacgagctc
      301 gtgacgaatc gcattcgggc gttcgtcgtc ctgcgcgaag gcactacgag tcgcgacgtc
      361 gccgcgttcg tcgccgaccg gttgccgaag tacatggtcc cggagcagtt ctcggagctc
      421 gacgtgctgc cgcgcacctc gacggggaag atcgaccgtc aagcgctcgc cggcattgcg
      481 tcggaggact gaggcagggg agcgcgcgga tggacttcgc gtggaccgaa gagcaggagg
      541 cctaccgcga cgtcgtcgtg aagttcgcga gcgcgctccc cgacgacgta cgcagccgcg
      601 atggtgacgg cgagttcgcc tgggatgcct ggaagcgctg cgctgcattc gggatccagg
      661 gcctcaatgt gccggaggaa tacggcggcg cggacgcgga cgcgctgacg acgatcgtcg
      721 cgatggaagc actcgggtac gggagccgcg acaacggtct gctcttctcg atcaacgcgc
      781 agatgtgggc gtgtcagcac ccgatcgtgc agttcggtac cgaggagcag aagcgcaagt
      841 atctgccccg cttgtgcgac gggtcgtcga tcgccgcgca cgggatgagc gagccgggct
      901 cgggctccga cgcgttcgcg ctggcgacga cagccgtgct cgatggcgac cactacattc
      961 tcaacggctc gaagatcttc gtgacgaacg cgccggtcgc agacctgttc gtcgtgttcg
     1021 cgacgacgaa ccccggcaag ggctttgccg ggctgtgcgg tttcctcgtc gaacgcgaca
     1081 cgccggggtt cacggtcggt cgaccactcg agaagatggg gctgcgcaca tcgccgatgg
     1141 ccgagctctt cttcgacgac gcgcgcgttc ccgtcgcgaa cctcatcggc aagccgcgcg
     1201 gcggcatggc catcttcaac gcggcaatgg aacgcgagcg cagtctcatc cttgcctgca
     1261 cgatcgggac catggagcgt ctcgtcgagg actctctggc ctacgcgaag caacgacgcc
     1321 agttcgggca gcggatcggc aagttccaag cggtgtcgca ccgcatcgtc gagatgaagt
     1381 tgcggctgga gacggcccgg ctcttgctgt accggctcgg gtggctgatc gaccagggcc
     1441 gcccgatcgg tctcgacgcc gccctcacga agctctacgt cagcgaatgc ttcgtgcagt
     1501 cgggcctgga cgcggtgcac gtgttcggcg gttacggcta catgcaggag tacgacgtcg
     1561 agcgcgtcgt gcgcgacgcg ctcggcagcc gcttgtactc cggcacttcg gacatccaac
     1621 gcaatctcgc cgccatgcgc ctaggtctgt gaccgcgctg ctcgttgtcg cggtcacatg
     1681 acgtggtatt tgcctggcct catcgcgcaa tcgcccgggc gggacaaccg caattcgtca
     1741 cgctgcgtga gaatgcgaca ggccgtatcc aaaccggcgt tcgcgacgtt tgccagttcg
     1801 ggcgggcagg ctccagaact gcgatcgtgc cggagagaca cgtgacaatc gaccagggcg
     1861 gcgtggggct gcggatcgag gaatcccggg tggcccggga actgcgacgg ctcgggatgc
     1921 ccgagctcgc cgtcgagcga gcggtggcgt cgggcctggg gctcacaccc gccgaagtca
     1981 ccgagttcac cgcccggccg ttcgccgccg acgaccgcag cgacgagatc gccgtcgtcg
     2041 agtcccagat ccggtcgctg cgcgatcagc tcggtatctg acgttcagcc gcgcgcggtc
     2101 gagccgcgtc gatgaagttg tcaaagagca gtcggggaaa tgaggcgcgc gcgaagtcag
     2161 ggatcgggtg accgcgcccc gaccggtacg ggccgacgcg ctcgcgactc cgtcgcaggt
     2221 tgcgatctct tgcgcgggag ttcgtagccc tgcgtcttgc gcttgtggtg caggtagcac
     2281 aaccaccgga gatttgccca tgaggtgagc ccgcctttgg cgaccgggtg ggagtggtcg
     2341 atctcgagat acccgcggtt gtggcaaccc tcgacatcgc actcgcgacc actcaccacc
     2401 aatgcggtgc gcagctccgc aggaatgtgt cgcccgaagt gcgcgaccgt cgtgatgtct
     2461 ttgcctttct tgatcacgaa cgtcaggaac gactcaccca acaggtcacg cgcccactgc
     2521 gcattcaccg gaccgacgcc ggggatctcg cacttcgcgc cgggcagctg gtcgccgacc
     2581 gccaggatgc tgtggtccat cacgagatgc gtggtgactt tggcggccgg cacctcggcg
     2641 ggccgtagga aggcattggc gaacacgtcg gcggcgtacg cctggtgtgg ctcgtgctcc
     2701 tcacctgcac gtcgcgaacg gaagatctgc tgcacgccct ggtcgacgat cgacttgatc
     2761 tgcccgccta cctcgggcgt cacggcaaag cgcccgacca tcattccgtc ggtgtcggtc
     2821 cacatccgga acgaccgcga tcgctgctgg cgcttcactc gttcctccgg gtcctcgacc
     2881 gcggcgcgca cggcaacaca cgcgtccttc
//
LOCUS       DASUGA010000064         2653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1510969, whole genome shotgun sequence.
ACCESSION   DASUGA010000064 DASUGA010000000
VERSION     DASUGA010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510969"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VFX21_01150"
     CDS             complement(<1..878)
                     /locus_tag="VFX21_01150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5094582.1"
                     /translation="MTDTAGGATRLVHVTTTDMSLELLLGPQLEAFAAAGYEVYGASA
                     PGRFVNALELRGITHIPLRHATRAMAIGEDARALGELVRIFRWLRPAIVHTHNPKPGV
                     YGRIAARVARVPIVVNTVHGLYALPEDRLRKRIPVYALERVAATFSQAELVQNPEDLP
                     VLRRLRLPESKMRLLGNGIDLGRFDVARRDQTAAWARRQLGATSPQSVVVGLVGRLVR
                     EKGYGEVFAAARTLRERHPELVFAVIGPDEPDKADAVTAAERADAAAAGIRFLGERGD
                     LERLYSGMDVYVLASYR"
     gene            complement(875..1666)
                     /locus_tag="VFX21_01155"
     CDS             complement(875..1666)
                     /locus_tag="VFX21_01155"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5094583.1"
                     /translation="MKRSAVAFDRVRPAPPGVVVLLYHRVSSVSNAQMYLPRGMFAKQ
                     IAKLAHANRVVSLSRALEMLSGSAPPPTPTPVVLTFDDGTEDFTKTVVPILQEFDVPA
                     TLYAATSYIEERRAFPDGAKPTSWAALADACSTGLVDVGSHTHSHRLLDRCPPQDVAD
                     ELDRSIGCIQERLGRAALDFAYPKAVPGSSHAEAAVKSRFRSAALAGTRANPYGRTDP
                     YRLARSPIQRSDGMRWYAHKVAGHMRLEDDLRHMLNRVRYSRAKT"
     gene            complement(1686..>2653)
                     /locus_tag="VFX21_01160"
     CDS             complement(1686..>2653)
                     /locus_tag="VFX21_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094584.1"
                     /translation="AIEYKYLGADMIGGFFCFGLLAFLGSYLWYRAVAEAIPFVNRRM
                     FCALLFFAPSLAFWPSSIGKEALMQLGFGLFALGVAKLLQHRLLNAALVAVPGGWLLW
                     VVRPHLLALAMAAGAVAYFAARSPKHMGASTRASLLRPVGLILMALLLVFAASSAAET
                     FGMKDFSLTSIENELNYATGQSEQGNSEFQTGNNSITPLSAAEGLVTVLLRPWPWEVD
                     SAYQILASLESFVFAMLIVLRFSSVSYSLTRWRAAPFLLYAWVTLLLYAVTFSSFSNF
                     GLLVRQRSLVLPALFVVLSIDPVLARRSEARDRSDTEPGGADAYA"
BASE COUNT          511 a          879 c          868 g          395 t
ORIGIN      
        1 cgataggacg caagcacgta gacgtccatc cccgagtaca ggcgctcgag atcaccacgt
       61 tcgccgagaa agcgtatgcc cgcggctgcc gcgtcagcgc gctccgcggc ggtcacggcg
      121 tcggccttgt cgggctcatc agggccgatc accgcgaata ccagctcggg atgccgttca
      181 cgaagtgtgc gcgccgccgc gaagacctcg ccgtagccct tctcgcgaac gagccggccg
      241 acgagcccga ccacgactga ctgcggtgat gttgcaccca actggcgccg agcccacgct
      301 gcggtttgat cccgacgtgc gacgtcgaag cgaccgagat cgatgccgtt gccgagcagc
      361 cgcatcttgg attccggcag gcgcaagcgg cgcagcaccg gcaggtcttc ggggttttgc
      421 accagctctg cctgcgagaa ggtcgcggcg acacgctcga gcgcgtagac gggtatgcgc
      481 ttacgaagtc ggtcctcggg caaggcgtac agcccgtgca cggtattcac gacgatcgga
      541 acccgcgcca cgcgagcggc gatccggccg tacacaccgg gcttcgggtt gtgggtgtgc
      601 acgatggccg gccgcaacca ccggaagatt cgtacgagct cgccgagcgc ccgcgcgtcc
      661 tctccgatcg ccatcgctcg cgtcgcgtgg cgcaacggga tgtgagtgat gcctcggagc
      721 tcgagcgcgt tgacgaaccg gcccggtgcc gacgcaccgt agacctcgta acccgcggcc
      781 gcgaacgctt cgagctgcgg ccccaacaac agctcgaggc tcatgtccgt cgtcgtgacg
      841 tgcacgagcc tcgtcgcccc accggccgtg tcggtcacgt cttcgcgcgt gagtaacgaa
      901 cacgattgag catgtgacgc aggtcgtcct cgagacgcat gtgaccggcg accttgtgcg
      961 cgtaccaccg catcccgtcg gaacgctgga tcggcgaccg cgccagccgg tacgggtcgg
     1021 tgcggccata cggattcgcg cgagtgccgg ccagcgcggc cgagcgaaac cgcgacttca
     1081 cggccgcctc ggcgtgcgac gagcccggca cggccttggg gtatgcgaag tcgagggcgg
     1141 cgcgcccgag tcgttcctgg atgcacccaa tcgaacgatc gagctcgtcc gcgacgtcct
     1201 gcggcgggca acgatcgagc agacggtggg agtgcgtgtg cgacccgacg tcgacgagcc
     1261 cggtgctgca cgcgtcggcc agggcggccc acgaggtcgg cttggcacca tctggaaagg
     1321 cgcgcctctc ctctatatag gaggttgccg cgtagagcgt ggccggcacg tcgaattctt
     1381 ggaggatcgg cacgaccgtc ttggtgaagt cctcggtccc gtcgtcgaac gtcaggacaa
     1441 ccggcgtcgg ggtcggcggc ggggcggagc cgctgagcat ctcgagcgcg cggctgagcg
     1501 acacgacgcg gttcgcgtgc gcgagcttcg cgatctgttt cgcgaacatc cctcgcggca
     1561 ggtacatctg cgcgttcgaa acggacgaga cgcggtgata cagcaacacg acgacaccgg
     1621 gcggcgccgg ccgcactcga tcgaacgcga ccgcggaccg cttcatgact ccacgagcta
     1681 tgccttcagg cgtacgcatc cgcccctcct ggttcggtgt cacttctgtc gcgcgcttcg
     1741 gaccgtcgcg cgagcaccgg gtcaatcgac aacacaacga acaacgccgg cagcacaagc
     1801 gaccgctgac gcaccaataa gccgaagttc gagaatgacg agaacgtgac tgcgtacagc
     1861 aacaacgtca cccacgcata caagaggaac ggcgcggccc gccatcgtgt cagcgaatat
     1921 gaaaccgacg agaagcgcag cacgatcagc atcgcgaaga cgaaggactc cagcgatgcc
     1981 aagatctgat acgcactgtc gacctcccac ggccatggtc gcaacagcac cgtgaccagt
     2041 ccctcggcgg cactcagtgg tgtgatcgag ttgttcccgg tttggaattc cgagttgcct
     2101 tgctcactct ggcccgttgc gtagttcagc tcgttctcga tcgacgtcag cgagaagtcc
     2161 ttcatcccga acgtctcggc ggctgagctc gcggcgaaga cgaggagcag cgccatgagg
     2221 atcaagccga ccggtcgcag cagcgacgcc cgggtggatg cgcccatgtg tttgggtgac
     2281 cgggccgcaa agtacgccac tgcccccgca gccatcgcga gcgcgagcag gtgcgggcga
     2341 accacccaca acagccaacc accgggcacc gcgacgaggg ctgcgttcaa caaccggtgc
     2401 tgcaagagct tcgcgacacc gagcgcaaac aagccgaagc ccagttgcat cagtgcttct
     2461 ttgccgatgg atgacggcca gaaggcgagg ctgggcgcga agaacagcag ggcacagaac
     2521 atgcgccggt tgacgaacgg aatcgcctcg gcgacggctc ggtaccaaag gtacgacccg
     2581 agaaaggcga gcaacccgaa gcagaagaaa ccgccgatca tgtcggcgcc caggtacttg
     2641 tactcgatgg cgg
//
LOCUS       DASUGA010000065         3179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1233645, whole genome shotgun sequence.
ACCESSION   DASUGA010000065 DASUGA010000000
VERSION     DASUGA010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233645"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /gene="disA"
                     /locus_tag="VFX21_01165"
     CDS             complement(<1..531)
                     /gene="disA"
                     /locus_tag="VFX21_01165"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF010009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HEX5094585.1"
                     /translation="MYGALRLVAPGTPLREGIDRILQAKMGALIVVGDGPDVLNICSG
                     GFLFDAEFTPQRLSELAKMDGAIILASDGSRVARANVHLVPDPDIPTTETGTRHRTAE
                     RVARQIAVPVITVSEEMSVVSIHYQGYKKTLEPIPRVLDRADQALQILGRYKARLDGV
                     SGALSALEVEDLVTVRD"
     gene            complement(699..2048)
                     /gene="radA"
                     /locus_tag="VFX21_01170"
     CDS             complement(699..2048)
                     /gene="radA"
                     /locus_tag="VFX21_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX5094586.1"
                     /translation="MARTHVRFHCDTCGFESPKWLGRCPDCGEWGGFGESAVAAVAAG
                     TGLVSKPLPIAQVDVLGAPRRPTGIGEVDRVLGGGLVGGSVTLLGGEPGMGKSTLLLQ
                     MLGALATSGVRALLVTAEESAAQVRLRAERLQTLAPNLMLVAETSLPAVCAHVDELRP
                     DVLAVDSIQSVHDPDSPGAAGSVSQVRDAAQRLVRLAKDLDVATVLVGHVTKDGALAG
                     PRVLEHVVDTVLSFEGDRHHELRLLRALKHRFGATDEIGIMGMTGRGLEPIADPSALF
                     LGDRRTGTSGSVVAPVLEGARPMCVEVQALVHVVHGGIPRRVTDAVDSNRLAMLQAVL
                     EHRGGCQLGAADVYASVAGGVRLSEPGSDLALLLALASAHRNRAVDAMTVAMGEVGLG
                     GEVRQVPQAPRRLAEAARLGFTRAIVPMSTPAVDGLDLVRVEDVQDALAVAEVGAWS"
     gene            2116..>3179
                     /locus_tag="VFX21_01175"
     CDS             2116..>3179
                     /locus_tag="VFX21_01175"
                     /inference="COORDINATES: protein motif:HMM:NF018452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEX5094587.1"
                     /translation="MAWHDFCTRLEAVGREIDGGAFGATDTDRALATRHLARQVVLAL
                     QGELEHGDPRTPAFHRYEEPWAQWGGPNPDNIYVRAAIDPDATYVVRGNVRGVRAAIF
                     SLCDGDMHMDKYGVFSERTLDTFGVEPDGELELHIGPTRQPGNWMATDPDARLLLIRQ
                     YQSDWQHDAIATFTIERADTTGRPPALPTSRAIDDALARAAEWVERSLTYWRDYADAA
                     RERMTPNTFGPAGTPKGGAPNIAYGAGWWKLSADEVLLVEFDVPDADYWSWTIHHRYR
                     LDSGDFASRQTSLNHTQVHVDGDGKVRLAVAATDPGVPNWIDTEGRPEGMVVFRSVGT
                     RTRPVPAGRVVPLDGLRAALP"
BASE COUNT          509 a         1154 c         1081 g          435 t
ORIGIN      
        1 atcgcgcacg gtcaccaggt cttcgacttc gagcgcggac aacgcaccgc tcacgccgtc
       61 gaggcgcgcc ttgtagcggc cgaggatctg cagcgcctgg tcggcgcggt cgaggacgcg
      121 cgggatcggc tcgagggtct tcttgtagcc ctggtagtgg atggagacga cgctcatctc
      181 ctccgagacg gtgatgaccg gcactgcgat ctggcgcgcg acacgctcgg cggtgcggtg
      241 ccgggtaccc gtctcggtgg tgggaatgtc ggggtcgggg acgaggtgca cgttcgcgcg
      301 ggcaacgcgc gatccgtcgg acgcgaggat gatcgcgccg tccatcttgg ccagctccga
      361 cagccgttgc ggcgtgaatt cggcgtcgaa caagaacccg ccggagcaga tgttcagcac
      421 gtcgggaccg tcgcccacga cgatgagcgc gcccatcttc gcctggagaa tccggtcgat
      481 gccctcgcgc agcggcgtac cgggcgcaac gaggcgaagc gcgccgtaca aggcttcaga
      541 tcgggccgac gccaagcgcg ctcctcccgg aaatcgaacg ccaaggggcg gttcgttcga
      601 caacttcggt gaagtcgagg gtagcgcctc cgggagcccc tgtcgccagg ccctcaggtg
      661 ccctcaggtg cctgcaaccg ggctccggtg cagttgcctc acgaccatgc gccgacctcc
      721 gcgacggcga gcgcgtcctg cacgtcttcg acgcgcacca ggtcgagccc gtcgacggcg
      781 ggtgtcgaca tcggcacgat cgcgcgcgtg aagccgagcc gggccgcttc cgcgagccgc
      841 cgcggcgcct ggggcacttg gcgcacttca ccaccgagac cgacctcgcc catggccacc
      901 gtcatggcgt cgacggcccg gttgcggtgc gcgctcgcca acgccaacag caacgcgaga
      961 tcggagcccg gctccgacag ccgaacgccc ccggccacgc tcgcgtagac gtcggccgcg
     1021 ccgagctggc atccgccgcg gtgctcgagc acggcttgca gcatcgccag ccggttggag
     1081 tcgacggcgt cggtcacgcg gcgcgggatg ccgccgtgca cgacgtggac gagcgcctgc
     1141 acctcgacgc acatcggacg cgcgccttcg agcaccggcg cgacgaccga gcccgacgtg
     1201 cccgtgcgcc gatcgccaag gaacagtgcg ctcggatcgg cgatcggctc caacccgcgg
     1261 ccggtcatgc ccatgatgcc gatctcgtcg gtcgcgccga accggtgctt cagcgcgcgc
     1321 agcaaccgca actcgtgatg gcgatcgccc tcgaacgaca acaccgtgtc gaccacgtgc
     1381 tcgagcacgc gcgggcccgc gagcgcgccg tctttggtga cgtgcccgac cagcacggtc
     1441 gcgacgtcga ggtccttggc cagccgcacc agccgctgcg cggcgtcgcg cacctgcgaa
     1501 accgagcccg cggcgcccgg cgagtcgggg tcgtgcacgg attggatgga gtcgaccgcg
     1561 agcacatcgg ggcgcagctc gtcgacgtgc gcgcacacgg ccggcagcga cgtctccgcg
     1621 acgagcatga gattcggcgc gagcgtttgc aggcgctctg ctcgcaggcg aacttgcgcg
     1681 gccgactcct ccgcggtgac cagcaacgcc cgcactcccg aggtcgcgag cgcaccgagc
     1741 atctgcagca acagcgtgct cttgcccatg ccgggttcgc cgccgagcag cgtcaccgac
     1801 ccgccgacga gcccgccgcc cagcacccgg tcgacctcgc cgatacccgt cggtcgccgc
     1861 ggtgcgccga gcacgtcgac ctgagcgatc ggcagcggct tcgacaccag cccggtgccg
     1921 gctgccaccg ccgcgaccgc ggactcgccg aagccgcccc actcgccgca gtcgggacag
     1981 cgcccgagcc acttgggcga ctcgaacccg caagtgtcgc agtggaaacg aacgtgtgtt
     2041 cgggccatgg cgaacacggt actgcgcggg tgtgacagcg aacggggatg ggtggcgtcg
     2101 tggtaggagt cggaggtggc ttggcacgac ttctgcactc ggctggaggc cgtcggccgg
     2161 gagatcgacg gcggcgcctt cggcgcgacc gacaccgacc gggccctcgc cacgcgccac
     2221 ctcgcccgcc aggtcgtgct cgcgttgcaa ggtgagctcg agcacggcga tccccgcacg
     2281 ccggcgttcc accgctatga agagccgtgg gcgcagtggg gtggacccaa tcccgacaac
     2341 atctacgtcc gcgccgccat cgatccggac gcgacctatg tcgtgcgcgg caatgtgcgc
     2401 ggtgtgcgcg ccgcgatctt ctcgttgtgc gacggcgaca tgcacatgga caagtacggc
     2461 gtgttcagtg agcgcacact cgacacgttc ggcgtggagc ccgacggcga gctcgaactg
     2521 cacatcggcc cgacccgaca acccggcaac tggatggcga cggatcctga cgcgcgcctg
     2581 ctgctgattc gtcaatacca atccgactgg caacatgacg cgatcgcgac gttcacgatc
     2641 gagcgcgccg acacaaccgg ccggcctccg gcccttccga cgtcacgtgc gatcgacgac
     2701 gcgctcgccc gcgcggccga gtgggtcgaa cgatcgctca cgtactggcg cgactacgcg
     2761 gacgccgcgc gcgaacgcat gacaccgaac accttcggac ccgcgggcac accgaagggc
     2821 ggcgcgccga acatcgccta cggcgcggga tggtggaagc tgtccgccga cgaagtgctc
     2881 ctcgtcgagt tcgatgtacc cgacgccgat tactggtcgt ggacgatcca ccatcggtac
     2941 cggctcgact ccggtgactt cgcaagccgc cagacgagcc tcaaccacac gcaggtgcac
     3001 gtcgacggcg acggcaaggt gcgcctcgcg gtcgccgcaa ccgatcccgg cgtgcccaac
     3061 tggatcgaca ccgaagggcg acccgaagga atggtcgtgt tccgctcggt cggcacccgc
     3121 acccggcccg tgcccgccgg tcgcgtcgtc ccactcgacg ggctgcgcgc cgcgcttcc
//
LOCUS       DASUGA010000066         3589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1790604, whole genome shotgun sequence.
ACCESSION   DASUGA010000066 DASUGA010000000
VERSION     DASUGA010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1790604"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VFX21_01180"
     CDS             <1..481
                     /locus_tag="VFX21_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5094588.1"
                     /translation="LGVDDVVVSEPKERRRELCAKLGARTVSPEELEQPVMPHDIVAE
                     PFDVVLECSGSNLAMEAGLAQLKRAGILVLVGAGIRKPKFDNNRILLNELVITGAFVY
                     DHDGFPRALELLASGKMPNDLLVEQEDYPLNRLLDAAVGLHEGELAAKAMIVPRVEA"
     gene            497..973
                     /locus_tag="VFX21_01185"
     CDS             497..973
                     /locus_tag="VFX21_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094589.1"
                     /translation="MPGPDFVTERKPRFNHVAMSLSPDDLDEEHRKLRAEFYEDVFGF
                     VDIPMMTVDRKRQVFTVHHVEQFVFLIADDDGGVMTCPRLDHFGVSVAEESELDDILA
                     RAKKWQARDDRVDIIDKHTDDHGMLAITSIYVKYLLPMMVEVQWWDYKQPKKTLAP"
     gene            970..1947
                     /locus_tag="VFX21_01190"
     CDS             970..1947
                     /locus_tag="VFX21_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX5094590.1"
                     /translation="MTQELRWGAFAPQGWKLEYTGWNAADAWARTKEITQRAEQLGYD
                     HIWVYDHVETVPRREATHCFEAFTTLSALAQHTTRIKLAQLVTCVAYRNVGLLAKEAA
                     CVDVFSGGRLILGLGAGWYEREYQAYGYPFPRPGVRLQILDETLEVVKRLWTEETVTY
                     QGKHLTLDGAYCDPKPIQQLPEIWVGGGGEKVTLRIEARHADKTNWQVGPDAFVHKSN
                     LLRQYCDEIGRDFDTIVRTHGPDCRVFASEADMKTWLDSPGGGQLWGRTPHDEYVRDN
                     LIGTVEQVAEKVQVYVDAGCREFIPWFRDYPSTDSLDAFIQGVVPLISA"
     gene            1968..3536
                     /locus_tag="VFX21_01195"
     CDS             1968..3536
                     /locus_tag="VFX21_01195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5094591.1"
                     /translation="MTVVSDAPAREPVAETETRPHPLVVFALAAVVAIGVGFFSYTRS
                     DLWLDEALSVNIARLPFSELRAALERDGAPPLYYALLHVWTSILGTSDVAVRSLSTVI
                     GLGLVTAVWFAARRFGSRSLAWSAVVLTFASPYAIRYATEARMYGLEMLFVALGIIAV
                     DRAFERPSYGRLLPITILSALLVYTQYWGFYILGVLGLVLLFIAWRKAAQRATALRIA
                     AAVVVGVLTFLPWIPTFMSQRAHTGTPWGDPVLPGLPIGETFLGFAGGEEQEGWLLLL
                     ILVPLMLLGAFGRRVDDRHVDIDVRVQRPVRGIAFMLAVTLVAGTTLSYLAGQAFEPR
                     YSAIVFPFFVILAARGVMTLGDRWIRVGVLVVVVSLGFAGGVRNVTEQRTQAGQVAAV
                     LKAEAQPGDVVVYCPDQLAPAVHRLLGDDRGLDEVTYPAFGPPAFVDWTDYQERLDAT
                     DHDAFAQDALARAEGKTLWYVTGPGYPNHHGVCEAMADSFGAAGRERIPRVLANEDYF
                     EKPGLVQFPAPTSS"
BASE COUNT          552 a         1191 c         1236 g          610 t
ORIGIN      
        1 gctcggcgtc gacgacgtcg tcgtgagcga accgaaggaa cggcggcgcg agctgtgcgc
       61 gaaattgggt gcgcgcacgg tgtcaccgga agagctcgaa cagccggtga tgccgcacga
      121 catcgtcgcg gagccgttcg acgtcgtgct cgagtgctcg ggcagcaacc tcgcgatgga
      181 agccgggctc gcgcagctga agcgcgcggg catcctcgta ctcgtgggtg ccggcatccg
      241 caagccgaag ttcgacaaca accgcatcct gttgaacgag ctcgtgatca ccggcgcgtt
      301 cgtgtacgac cacgacggtt tcccgcgcgc attggagctg ctcgcgtcgg gcaagatgcc
      361 gaacgacctg ctcgtcgagc aggaggatta tccgctgaac cgtttgctcg acgccgccgt
      421 cgggctgcac gaaggcgagc tcgcggcgaa ggcgatgatc gtgccgcgag tcgaggcgta
      481 gggaggagac cgcgtcatgc ccggtccgga tttcgtgacg gagcgcaagc cgcgcttcaa
      541 ccacgtggcg atgagcctgt cgcccgatga cctcgacgag gagcaccgca agctgcgggc
      601 cgagttctac gaggacgtct tcggtttcgt ggacatcccg atgatgaccg tcgatcgcaa
      661 gcggcaggtc ttcacggtcc accacgtcga gcagttcgtg ttcctcatcg cggacgacga
      721 cggcggcgtg atgacgtgcc cgcgtctcga ccacttcggc gtctcggtcg cggaggagtc
      781 ggagctcgac gacatcctgg ctcgcgcgaa gaaatggcaa gcgcgcgacg accgggtcga
      841 catcatcgac aagcacaccg acgaccacgg catgctcgcg atcacgagca tctacgtgaa
      901 gtacctcttg ccgatgatgg tcgaagtgca atggtgggac tacaagcaac cgaagaagac
      961 gctcgccccg tgacacaaga acttcgttgg ggcgcgtttg ccccgcaggg ctggaagctc
     1021 gaatacaccg gttggaacgc ggcggacgcg tgggcacgca cgaaggagat cacccagcgc
     1081 gcggagcagc tcggctacga ccacatttgg gtgtacgacc acgtcgagac ggtgccgcgc
     1141 cgcgaggcca cgcattgctt cgaggcgttc acaacgttgt cggcgctcgc gcagcacacg
     1201 acgcgcatca agctcgcgca gctcgtcacc tgcgtcgcgt accgcaacgt cgggttgttg
     1261 gcgaaggagg ccgcgtgcgt cgacgtgttc tccggcggcc ggctcattct cggtctgggc
     1321 gcgggctggt acgaacgcga gtaccaggcg tacggatacc cgttcccgcg tcccggtgtg
     1381 cggctgcaga tcctggacga gaccctcgaa gtcgtgaagc ggctgtggac cgaggaaacg
     1441 gtcacgtacc agggcaagca cctcacgctc gacggcgcgt actgcgatcc gaagccgatc
     1501 cagcagctgc ccgagatctg ggtcggcggc ggcggcgaga aggtgacgct gcgcatcgag
     1561 gcgcgccacg ccgacaagac caactggcaa gtcggtccgg acgcgttcgt gcacaaatcg
     1621 aatctgctgc ggcaatactg cgacgagatc ggccgtgact tcgacaccat cgtgcgcacg
     1681 cacggccccg actgccgcgt cttcgcgtcc gaagccgaca tgaagacgtg gctcgactcg
     1741 ccgggcggcg ggcagctgtg ggggcgtaca ccgcacgacg agtacgtgcg cgacaacctg
     1801 atcggtaccg tcgagcaggt tgccgagaag gtgcaggtct acgtcgacgc cggctgtcgc
     1861 gagttcatcc cgtggttccg cgactacccg tcgaccgatt ccctcgacgc gttcatccag
     1921 ggcgttgtac cgctgatttc tgcctgagcg gggtagcctc gccgcccgtg accgtcgtct
     1981 ccgacgcgcc cgcacgcgag cccgtcgccg agacggaaac gcggccgcat ccactggtcg
     2041 tcttcgcgct cgcggccgtc gtcgccatcg gtgtcggctt cttctcgtac acgcgctccg
     2101 acctgtggct ggacgaagcg ctctcggtca acattgcccg gttgccgttc agcgagctgc
     2161 gggccgcgct cgaacgcgac ggtgcgccac ccctctatta cgcgctgttg cacgtgtgga
     2221 cgagcatcct cggcacctcc gacgtcgcgg tgcggtcgct gtcgacggtc atcgggctcg
     2281 gcctcgtcac cgcggtgtgg ttcgcggccc gccgtttcgg gtcgcggtcg ttggcgtgga
     2341 gcgcggtcgt gctgacgttc gccagcccgt acgcgatccg ttatgcgacc gaagcgcgga
     2401 tgtacggcct ggagatgttg ttcgtcgcgc tcggcatcat cgcggtcgac cgcgcgttcg
     2461 agcgcccgtc gtacggccgg ctgctgccga tcaccatcct cagcgcgctg ttggtgtaca
     2521 cgcagtactg ggggttctac atcctcggcg tgcttggcct ggtgctcttg ttcatcgcgt
     2581 ggcgcaaggc tgcgcaacgc gcgaccgcgc tgcgcatcgc ggctgcggtc gtggtgggcg
     2641 tgctcacgtt cctgccctgg atcccgacgt tcatgtcgca gcgcgcgcac accggaacgc
     2701 cgtggggcga cccggtcttg ccgggcctgc cgatcggcga gacgttcctc ggcttcgcgg
     2761 gcggcgagga gcaagagggc tggctcctcc tcttgatcct cgtgccgctc atgctgctgg
     2821 gagccttcgg ccgtcgcgtc gacgaccggc acgtcgacat cgacgtgcgc gtgcagcgcc
     2881 cggtgcgcgg catcgccttc atgctcgcgg tgacgctcgt cgcgggcacg acgctgagct
     2941 acctcgcggg gcaggcgttc gaaccgcggt actccgcgat cgtgttcccg ttcttcgtga
     3001 tcctcgcggc ccgaggcgtc atgacgctcg gcgaccggtg gatccgggtc ggcgtgctgg
     3061 tggtcgtcgt ctcgctgggg ttcgcgggcg gcgtgcgcaa tgtcaccgag caacgcacgc
     3121 aggccggtca ggttgccgcg gtcttgaagg cggaagcgca acccggtgac gtcgtcgtct
     3181 actgccccga ccagctcgcc cctgcggtgc accgcctgct cggcgacgac cgcggtctcg
     3241 acgaggtgac gtaccccgcg ttcggcccgc cggccttcgt cgactggacc gactaccagg
     3301 agcggctcga cgcgaccgat cacgacgcgt tcgcgcagga cgcgctggcc cgcgccgagg
     3361 gcaagacgct ctggtacgtg accggtcccg ggtatccgaa ccatcacggt gtgtgcgagg
     3421 cgatggccga cagcttcggc gcggccgggc gcgagcggat cccgcgcgtc ctcgcgaacg
     3481 aggactactt cgagaagccc ggtctggtgc agttcccggc gcccacctcg tcgtgacgtc
     3541 gttgctgacg cggggaccca cgcgcggcga catggtgcgc gcggtgctg
//
LOCUS       DASUGA010000067         5883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_262818, whole genome shotgun sequence.
ACCESSION   DASUGA010000067 DASUGA010000000
VERSION     DASUGA010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262818"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1859
                     /locus_tag="VFX21_01200"
     CDS             <1..1859
                     /locus_tag="VFX21_01200"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX5094592.1"
                     /translation="TLLVSPLLALMRDQIRAAQAIGIRAETINSTNRDAWEPIATAVQ
                     AGEVDILLISPERLNNAQFRRDVLPELAGSVGLLVVDEAHCISDWGHDFRPDYRRVRD
                     VIAGLGDDVPVLATTATANARVTTDVAEQLGGEPLVLRGSLARESLRLSVAVMPERAQ
                     RLAWLAHHIPSLPGSGIVYCLTVGDAERVADWLRTRGIDAAAYTGQTEPTERERIEAA
                     LRANELKVVVATTALSMGYDKPDLAFVVHYQSPSSPIAYYQAVGRAGRAILTANVMLL
                     AGDEDRAIWDYFDSTAFPPRALVESVLDIVERAGGPVSANTIQAEANIGAGRLDALLK
                     VLDVEGAVARTAEGWERTGAPWHYDTDRLERVEAARAEEAAAMLAYQRTDECLMAFLR
                     TQLDDVDIAPCGRCANCTDEPAPSDLPPALVADAITFLRGIDYVIEPRKRWPNGSPVV
                     RGNIGKERQCAPGRALAERGDGGWAATVESLLAGRATADDDITDAIARVLKRWDWAAR
                     PTWITFVPSRNEARQAVLADVAERLGALGHLPVHDVIRRTRDGRPQHTLVNSAHQVAN
                     VLDAFTIEGPIPEGPVLLLDDRIASGWTFAVVGALLRDAGAEAVLPFALLSA"
     gene            complement(1930..3000)
                     /locus_tag="VFX21_01205"
     CDS             complement(1930..3000)
                     /locus_tag="VFX21_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255838054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(2647..2649),aa:Sec)
                     /transl_table=11
                     /product="ResA-like WAxxUGC motif-containing protein"
                     /protein_id="HEX5094593.1"
                     /translation="MILDELVVDADTFAARTGWDMKQQGACKGEVCVPMPAEMHVDGG
                     RLDVRVVSERLGMPLVRDDTHGVWALGPETAVTGRVLTTAQMPDVVLPDADGNPFALS
                     TLRGQKVLLVAWASWUGCRFDLPVWETLRQEFHPHGLEIVTVALDVDPDAARPFIEGA
                     QPSHPSLIDQAHVLDELVGVVNVPNGMWIDEQGVIVRPPEPAFPGRNPATESFRTMDL
                     STLPPDLADVLREARKITTDPEGYVAMLRDWVANGAASSFALRPDEVIARSHPRANDE
                     AIAAAEFELGQYLHCAGDHAAAIPHWREAHRRHPDNWTYKRQAWNFEDPVQQRRTDAY
                     DSSWYEDIKKIGAENYYPPVLP"
     gene            3102..3422
                     /locus_tag="VFX21_01210"
     CDS             3102..3422
                     /locus_tag="VFX21_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014674007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX5094594.1"
                     /translation="MPKYLVIASYTAEGAKGVLAKGGSARRDAVDKMVSDLGGTVESF
                     YFAFGADDVFVTIDLPDNVAAAALGLAVGASGATALRTTVLLTPEEIDRASQVTVAYE
                     TPGS"
     gene            complement(3522..3800)
                     /locus_tag="VFX21_01215"
     CDS             complement(3522..3800)
                     /locus_tag="VFX21_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094595.1"
                     /translation="MATRRTSFDKLQRERAKRAKAADKRERRQERAAGGEDDQDVVAP
                     IEGELSAGELMQRLEALHARYEAKTLDFDSFEEQKLELLERLAALPMD"
     gene            complement(3953..4312)
                     /locus_tag="VFX21_01220"
     CDS             complement(3953..4312)
                     /locus_tag="VFX21_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HEX5094596.1"
                     /translation="MVGVAKNVLGGELEPCGLDPLTGFYRDGCCETGGEDVGVHVVCA
                     RMTDEFLAFSREQGNDLSTPRPGFAGLVAGDRWCLCASRWHEAFKAGVAPPVVLEATH
                     ALALEWCSIDALRAHAV"
     gene            4345..5316
                     /locus_tag="VFX21_01225"
     CDS             4345..5316
                     /locus_tag="VFX21_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HEX5094597.1"
                     /translation="MAVDPAVPVVDVAPLMTGAGAGDVDAVAAALDRACRDTGFFTIV
                     GHGVDPRCVARVDRLSREFFGLDDEEKARIAMAHGGRAWRGWFPLGGELTSGRPDRKE
                     GLYFAAEHPGDARPLHGANLFPERPVEMRAAVLEYLDAMTRLGHQVMRGLARALGLDD
                     DWFAGHLTGDPTVLLRVFRYPPHAGDDAWGVGEHTDYGLLTMLAQDDAGGLEVRTRTG
                     WRAVPPVRDALVCNIGDMLDRMTGGEYQSTAHRVRNTSDRDRIAIPFFFDPAWDARVR
                     ALPGRRGDVTGGDRWDGQDLHGFEGTYGDYLVAKVSKVFPALREGVL"
     gene            5313..>5883
                     /locus_tag="VFX21_01230"
     CDS             5313..>5883
                     /locus_tag="VFX21_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX5094598.1"
                     /translation="MTVRIGLVLGAGGVVGHAWHAGVLRALQAESGWDPRDADVIVGT
                     SAGSVVGAALRAEIDAADIGPRRDGPAFASGPRSGLPRMAAPGMFVRQVMRPWDARVG
                     LLSAALLPEGRVSTEVVAAGVRARFGDDWPPRALFVNAVRLDTGQRVTFGRDGAPRTD
                     VATAVAASIAIPGFFAPVTIDGVRYVDGGA"
BASE COUNT          850 a         2037 c         2076 g          920 t
ORIGIN      
        1 cgacgctgct cgtctcaccg ttgctggcgc tgatgcgcga ccagatccgc gctgcgcagg
       61 cgatcgggat ccgggccgag acgatcaact cgaccaaccg cgacgcgtgg gagccgatcg
      121 caactgcggt gcaggcgggc gaggtcgaca tcttgttgat ctcgcccgaa cggttgaaca
      181 acgcacagtt ccgtcgcgac gtgttgcccg agctcgcggg gagcgtgggg ctgttggtcg
      241 tcgacgaagc gcattgcatc tcggattggg gtcacgactt ccgccccgac taccggcgcg
      301 tacgcgacgt cattgccggc ctcggcgacg acgttcccgt gctcgcgacg acggcgaccg
      361 cgaacgcgcg ggtgaccacc gacgtcgccg agcagctcgg cggggagccg ctcgtgctgc
      421 gcggttcgct cgcccgtgag tcactgcggc tgtcggtcgc ggtaatgccc gagcgggccc
      481 aacgcctggc ctggctcgca caccacatcc cgtcactgcc gggcagcggc atcgtctact
      541 gcctcacggt cggcgacgca gaacgcgttg ccgactggct gcgcacacgc ggcatcgacg
      601 cggctgccta cacgggccag accgagccca cggagcggga gcgcatcgag gcggcgctgc
      661 gggccaacga actgaaggtc gtcgtcgcga cgaccgcgct gtcgatgggt tacgacaagc
      721 ccgacctcgc gttcgtcgtg cactaccaat cgccgtcgtc accgatcgcg tactaccagg
      781 ccgtcggacg agcggggcgc gcgatcttga cggccaacgt catgttgctc gcgggcgacg
      841 aagaccgcgc catctgggat tacttcgact cgaccgcgtt cccgccgcgc gcgttggtcg
      901 aatcggtgct cgacatcgtc gagcgtgcgg gcggtccggt gagcgcgaac acgatccagg
      961 ccgaggcgaa catcggtgcc ggccggctcg acgcactcct caaggtgctc gacgtcgaag
     1021 gcgcggtggc gcgcactgcc gagggctggg aacgcaccgg tgcgccatgg cactacgaca
     1081 ccgaccggtt ggagcgagtc gaagcggcgc gcgccgagga ggccgccgcg atgctcgcgt
     1141 accaacgcac cgacgaatgc ttgatggcgt tcctgcgcac gcaactcgac gacgtcgaca
     1201 tcgcaccgtg cggccgctgc gcgaactgca ccgacgaacc ggcaccgtcc gacctcccgc
     1261 cggcattggt ggccgacgcg atcacgttcc tgcgcgggat cgactacgtc atcgaaccgc
     1321 ggaagcgctg gcccaacggc agtcccgtcg tgcgcggcaa catcggcaag gaacgccagt
     1381 gcgcacccgg ccgcgcgctc gccgaacgcg gcgacggcgg ctgggccgcg accgtcgagt
     1441 ccttgctcgc gggccgggca accgccgacg acgacatcac cgacgcgatc gcgcgggtgc
     1501 tcaagcgctg ggactgggcg gcgcgcccga cgtggatcac gttcgtgccg tcgcgcaacg
     1561 aggcgcgcca agcggtgctc gccgacgtcg ccgaacggct cggcgcgctc ggccacctgc
     1621 ccgtgcacga cgtcatccgc cgcacgcgcg acggccgccc gcagcacaca ctcgtcaaca
     1681 gcgcgcatca ggtcgcaaac gtgctcgacg ccttcaccat cgagggcccg atcccggagg
     1741 gtccggtgct gctcctcgac gaccgcattg cgtccggctg gacgttcgcc gtcgtcggcg
     1801 ccctcctcag agacgcggga gccgaggccg tcctcccctt cgccctgctg tcagcctgac
     1861 gccgcgctcc tccgagcctt ctttggaacc ctggcccccg aatcgggggc cagattgaca
     1921 aaggaagcgc tacgggagga cgggtgggta gtagttctcg gcgccgatct tcttgatgtc
     1981 ctcgtaccag gagctgtcgt acgcgtcggt gcggcgttgc tgcacggggt cttcgaagtt
     2041 ccacgcttgg cgcttgtacg tccagttgtc ggggtggcgg cggtgcgctt cccgccagtg
     2101 ggggatcgcg gccgcatggt cgcccgcgca gtgcaggtac tggcccagct cgaactcggc
     2161 cgccgcgatc gcttcgtcgt ttgcgcgcgg atgcgaacgg gcgatgacct cgtcggggcg
     2221 caacgcgaac gagctggcgg cgccgttcgc gacccagtca cgcagcatcg cgacgtagcc
     2281 ctccgggtcg gtcgtgatct tgcgcgcctc tcgcaacacg tcggcgaggt caggcggcag
     2341 cgtcgagaga tccatcgtgc ggaacgactc ggtggcggga ttgcggccgg gaaacgccgg
     2401 ctcgggtggc cgcacgatga cgccttgctc gtcgatccac atgccgttgg gaacgttgac
     2461 gaccccgacc aactcgtcga gcacgtgcgc ttggtcgatc aacgacgggt gtgacggctg
     2521 cgcgccctcg atgaacgggc gcgccgcgtc ggggtcgacg tcgagcgcga cggtgacgat
     2581 ctcgaggccg tgagggtgga actcttggcg cagcgtctcc cacacgggga ggtcaaagcg
     2641 gcagcctcac cacgacgccc acgcgacgag cagcaccttc tggccgcgca aggtcgacaa
     2701 tgcaaacggg ttgccgtcgg catccggcaa cacgacgtcg ggcatctgcg cggtcgtgag
     2761 cacccgcccg gtgaccgcgg tctcgggacc gagcgcccac acaccgtgcg tgtcgtcgcg
     2821 cacgagcggc atgccgaggc gttcggaaac gacgcgaacg tccaagcggc caccgtcgac
     2881 gtgcatctcg gccggcatgg gcacgcagac ctcgcccttg cacgcaccct gctgcttcat
     2941 gtcccaaccg gtgcgcgccg cgaacgtgtc ggcgtcgacc accaactcgt cgaggatcat
     3001 cgacgcaccg tagcgcgccg aacgcgacgt cgattgtggc ccttgcaggc gtttgtcgcg
     3061 cggtggagac tcccggcgcc tcgatgaggg gaggggcaca catgcccaag tatctggtga
     3121 tcgcttccta cacagcagaa ggtgcaaagg gcgtactggc caaaggtgga tcggctcgtc
     3181 gggacgccgt cgacaagatg gtcagcgatc tcggcggtac cgtcgagagc ttctacttcg
     3241 cattcggtgc cgacgacgtg ttcgtcacca tcgatctacc ggacaacgtc gcggcggcag
     3301 cactcgggct tgccgtgggt gccagcggag cgacggcgtt gcggacgact gtgttgctca
     3361 cgccggaaga gatcgaccgg gcctcgcagg tcacggtcgc atacgagacg cctggttcgt
     3421 aggacgggcg catgcggggc cggccgtcat ggacggctgg tcgcgcgtgc cgctcgtctc
     3481 gctgcgtgac ctccgatggc ggcggccgcg gcctcgccgg cctagtccat ggggagcgcg
     3541 gcgaggcgct ccagcagctc gagcttctgc tcttcgaagc tgtcgaagtc gagtgtcttc
     3601 gcctcgtacc gagcgtgcag cgcttcgagg cgctgcatca gctcacccgc cgagagctca
     3661 ccctcgatcg gcgcgacgac gtcctggtcg tcttcgccgc cggcggcccg ctcctggcgt
     3721 cgctcgcgct tgtccgcggc ctttgcccgc tttgctcgct cccgctggag cttgtcgaaa
     3781 gaggtgcgtc gtgtggccat tgcattctcc gtcgtaccgt gtcgtgtcat cgtgcctcgc
     3841 gaaggcgcgt cagcacgggc aatccgattt ctgtcggtcc ttccaggcta cgtcgcgcga
     3901 gcactcgttc ggtcaccggg ccggatgacc agcccaaatg tgtcacaacc cgtcagactg
     3961 catgggctcg caatgcgtcg atcgagcacc attcgagcgc gagcgcatgc gtggcttcga
     4021 gcacgaccgg cggtgcgacg ccggccttga acgcctcgtg ccagcgggac gcgcacaggc
     4081 accagcgatc gcccgccacc agtcccgcga acccggggcg cggcgtgctg aggtcgttgc
     4141 cctgctctcg cgagaacgcg aggaactcgt cggtcatgcg ggcgcagacg acgtggaccc
     4201 cgacgtcttc gccgcccgtc tcgcagcagc cgtcgcggta gaagccggtc aggggatcca
     4261 agccacacgg ttccagctcg ccgccgagca cgttcttcgc cacgccgacc atgcaagcag
     4321 gttgcgaccg ttagcgtcac gctcgtggcc gtggaccccg cagtgccggt cgtcgacgtc
     4381 gcgccgctca tgaccggtgc cggcgcgggc gacgtcgacg cggtcgcggc cgcgctcgac
     4441 cgcgcgtgcc gcgacacggg tttcttcacg atcgtcgggc acggcgtcga cccgcggtgc
     4501 gtggcgcgcg tcgaccggtt gtcgcgggag ttcttcgggc tcgacgacga agagaaggcg
     4561 cgcatcgcga tggcgcacgg tggacgcgcc tggcggggct ggttcccgct cggcggtgag
     4621 ctgacttcgg ggcgacccga ccgcaaagag ggcctctact tcgccgccga gcacccgggc
     4681 gacgcgcgac cgttgcacgg cgcgaacctg tttcccgagc gaccggtgga gatgcgcgcg
     4741 gccgtgctcg agtatctcga cgccatgacg cgcctggggc accaggtgat gcgtggcctc
     4801 gcgcgggcgc ttggcctcga cgacgattgg ttcgcgggcc atctcacggg cgatccgacg
     4861 gtgttgctgc gggtgttccg ctatccgccc catgccggcg acgacgcgtg gggcgtcggg
     4921 gagcacaccg actacggcct gctgacgatg cttgcccaag acgacgcggg cgggctcgag
     4981 gtgcgcacgc gtaccgggtg gcgggccgtc ccgcccgtgc gcgacgcctt ggtgtgcaac
     5041 atcggagaca tgctcgaccg catgaccggc ggcgagtacc agtcgaccgc gcatcgagta
     5101 cggaacacga gtgaccgcga tcggatcgcg atcccgttct tcttcgatcc cgcgtgggac
     5161 gcgcgggtgc gcgcgctgcc cggtcgtcgc ggcgacgtga cgggcggcga ccgatgggac
     5221 ggacaggatc tgcacggctt cgaaggaacc tacggcgact acctcgtggc caaggtctcg
     5281 aaggtgttcc ccgcgctgcg cgagggagtg ttgtgacggt ccgcatcggc ctggtactgg
     5341 gcgcgggcgg tgtggtcggg cacgcgtggc atgccggcgt cttacgcgcg ctgcaagcag
     5401 agtccggctg ggaccctcgc gacgcggatg tgatcgtggg gacgtccgcg ggctccgtcg
     5461 tcggcgccgc gctgcgggcg gagatcgacg ccgccgacat cggcccgcgc cgcgacggcc
     5521 ccgcgttcgc gtccgggccc cgttcgggtc tcccgcgcat ggccgcgccg ggcatgttcg
     5581 tgcgccaggt gatgcggccc tgggatgcgc gcgtcggtct cctcagcgcc gcgctgctgc
     5641 ccgaagggcg cgtctcgacc gaggtcgtgg ccgcgggcgt tcgtgcccgc ttcggcgacg
     5701 actggccgcc gcgtgcgctc ttcgtgaacg ccgtgcggct cgacaccggg cagcgcgtga
     5761 cgttcggccg ggacggcgcg ccgcgcaccg acgtcgcaac ggcggtcgcg gcatcgatcg
     5821 cgattccggg cttcttcgca ccggtgacga tcgacggcgt tcgctacgtc gacggcggcg
     5881 cgc
//
LOCUS       DASUGA010000068         2737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_817029, whole genome shotgun sequence.
ACCESSION   DASUGA010000068 DASUGA010000000
VERSION     DASUGA010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817029"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VFX21_01235"
     CDS             <1..224
                     /locus_tag="VFX21_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094599.1"
                     /translation="LRQHWTVDDAVDALAVLTSYATYERLRRADRSPEQVEAVLAKLA
                     ISIVAPGTGTNSTGAAAPATATATAGHAN"
     gene            complement(234..662)
                     /locus_tag="VFX21_01240"
     CDS             complement(234..662)
                     /locus_tag="VFX21_01240"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX5094600.1"
                     /translation="MTDWHEVPKYLVPHPTPEDREFWAGARRGELRIQHCTTCDKCQH
                     YARLFCSHCGAGTLEWVTASGTGTIYSFTVIRQNGVPPFNERVPFVIAAVDLDEAGAR
                     VLGAMPDLAPDDARIGMRVHATFREASEEFGFVDFAASPG"
     gene            complement(659..1828)
                     /locus_tag="VFX21_01245"
     CDS             complement(659..1828)
                     /locus_tag="VFX21_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009738259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEX5094601.1"
                     /translation="MPTHRNVAIVGVGLADGPHATGLTSTMLAAQGFQRALADAGLTI
                     KDVDGLASAGNTAMHEVGLAEYFGIRPRWLESTSCGGSSFEFHAMHAYRAIQNGDADT
                     IAIVYGSNQLSAAGRTLGTGGGRGGGGGQMPLSMSYEFPTGITLVGSYAMAAQRHMYQ
                     YGTTPEQLASIAVQMREHAGRNPDAMYRDPITVDDVMSSRLVADPLHKLDCCVISDGG
                     CCIILTTEERARDLTHYPVFVRGAAGGTTHNSITSMEDMTTTAGAISGPKAFAEAGIT
                     PSDVDMFAMYDSFTYTVLVMLEDLGFAPKGEGGAFAADGNLRLGGRLPTNTDGGGLSC
                     THPGMRGLFLLCEATRQLRGEAGDSQVDGAEIAVAHGSGGWLSTQATVVLGTEAA"
     gene            1851..2597
                     /locus_tag="VFX21_01250"
     CDS             1851..2597
                     /locus_tag="VFX21_01250"
                     /inference="COORDINATES: protein motif:HMM:NF015123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5094602.1"
                     /translation="MQQPPTHVAHNRAFWDADADDYQAAHGADLEARALAWGAFRAPE
                     SELGVLGDVVGRDLLELGCGGAQWSAALAARGARCTGLDLSGAQLAHARAHAPILPLV
                     QASGEMLPFRARAFDIVFCDHGATSFCDPAHVLPEVARVLRPGGLFAFCVTDPLVYLT
                     WDNDKQKQTKKLRLEVDDLGRVDNGCGTVDWVSTPGQWIANFRAHGFTIETLHELRPS
                     EGATTTYPDFVPYKWARRWPAEQIWRVRRV"
BASE COUNT          408 a          951 c          960 g          418 t
ORIGIN      
        1 agctgcgcca gcactggacc gtcgacgacg ccgtcgacgc gctcgcggtg ttgacgtcgt
       61 acgcaaccta cgaacggctg cggcgcgccg accgttctcc cgaacaagtc gaggcggtgc
      121 tcgccaagct cgcgatctcg atcgttgcgc cgggcaccgg caccaactcg accggtgccg
      181 cggcgccggc gaccgcgacg gcgaccgcgg ggcacgcgaa ctgagccgct gcgtcaaccc
      241 ggcgacgccg cgaagtcgac gaacccgaac tcctcactcg cttcgcggaa cgtcgcatgc
      301 acgcgcatcc cgatgcgtgc gtcgtcgggc gcgaggtcgg gcatcgcgcc gagcacgcgt
      361 gcacccgctt cgtcgaggtc gacggccgcg atgacgaacg gcacgcgctc gttgaacggt
      421 ggcacaccgt tctggcgtat cacggtgaac gaatagatcg ttcccgtgcc gctcgcggtg
      481 acccattcga gcgtgccggc gccgcagtga ctgcagaaca gccgcgcgta gtgctggcac
      541 ttgtcacacg tcgtgcagtg ctggatgcgc aactcgcccc gccgcgcgcc ggcccagaac
      601 tcgcggtcct cgggcgtcgg gtgcggcacg aggtacttcg ggacttcgtg ccagtcggtc
      661 acgccgcctc cgttccgagc accacggtgg cttgcgtgga caaccaaccg ccgctgccgt
      721 gggcgacggc gatctcggcg ccgtcgacct gactgtcgcc tgcttcgccg cgcagctgac
      781 gcgtcgcctc gcagagcagg aacagcccgc gcatgccggg atgtgtgcac gagaggccgc
      841 cgccgtcggt gttcgtcggc aggcgcccac cgaggcgcag gttgccgtcg gccgcgaacg
      901 cgccgccctc gcccttcggc gcgaagccga ggtcttcgag catgaccagc accgtgtagg
      961 tgaacgagtc gtacatcgcg aacatgtcga cgtcggacgg cgtgatgccc gcttccgcga
     1021 acgccttcgg accgctgatc gcgccggcgg tcgtcgtcat gtcttccatg ctcgtgatcg
     1081 agttgtgcgt cgtgccgccg gccgcgccgc gcacgaacac cgggtagtgc gtgaggtcgc
     1141 gggcgcgctc ctcggtcgtc aggatgatgc agcagccgcc gtcggagatc acgcagcagt
     1201 cgagcttgtg cagcgggtcc gcgacgagcc gtgacgacat gacatcgtcc acggtgatcg
     1261 gatcgcggta catcgcgtcg ggattgcggc ccgcgtgctc gcgcatctgc accgcgatcg
     1321 acgcgagctg ctcgggcgtg gtgccgtact ggtacatgtg ccgctgcgcg gccatcgcgt
     1381 acgacccgac gagcgtgata ccggtcggaa actcgtacga catgctcaac ggcatctgcc
     1441 cgccaccacc gccgcgaccg ccgccggtgc cgagcgtgcg gcccgccgcg gacagctggt
     1501 tgctgccgta gacgatcgcg atcgtgtcgg cgtcaccgtt ctggatcgcc cggtaggcgt
     1561 gcatggcgtg gaactcgaag ctggagcctc cgcacgacgt cgactcgagc cagcggggcc
     1621 ggatgccgaa atactcggcc agcccgacct cgtgcatcgc ggtgttgccg gcgctcgcga
     1681 gcccgtcgac gtccttgatc gtcagccccg cgtccgcgag cgcacgctgg aagccctgcg
     1741 cggcgagcat ggtcgaggtc aaaccggtgg cgtggggccc gtccgcgagc ccgacgccga
     1801 cgatggccac attgcgatgg gtcggcacgg cggggcaacg tatccgattc atgcaacaac
     1861 cgccgacaca cgttgcgcac aaccgcgcgt tctgggacgc ggacgcggac gactaccaag
     1921 ccgcccacgg cgccgacctc gaggcgcggg cgttggcgtg gggcgcgttc cgcgcgccgg
     1981 agtccgagct cggcgtcctc ggtgacgtcg tcgggcgcga cctgctcgag ctcgggtgcg
     2041 gcggcgcgca atggtcggcg gcgctcgcgg cacgtggggc gcgctgcacc ggcctcgacc
     2101 tgtcgggcgc gcagctcgcg cacgctcgtg cacacgcgcc gatcctgccg ctcgtgcagg
     2161 caagcggtga gatgctgccg tttcgggcgc gggcgttcga catcgtgttc tgcgaccacg
     2221 gcgcgacgag cttctgcgat cccgcgcacg tgctcccgga ggtggcacgc gtgcttcgcc
     2281 ccgggggctt gttcgcgttc tgcgtcaccg atccgctcgt gtacctgacg tgggacaacg
     2341 acaagcagaa gcagacgaag aagctgcggc tcgaggtcga cgacctcgga cgcgtcgaca
     2401 atggctgtgg caccgtcgac tgggtctcca cacccggcca gtggatcgcg aacttccgcg
     2461 cccacggctt cacgatcgag acgctgcacg agctgcgccc atccgaaggg gcgacgacga
     2521 cctatccgga tttcgtcccg tacaagtggg cgcgccgctg gcccgcggag cagatctggc
     2581 gcgtccggcg cgtttagtgc tccgtccggt gacgttgtcg atgttcacgg cgagtcaggg
     2641 gcgtcgctcg caaggacgca cgacgaagga agagtgggcc tctttcgagg aggaggacgc
     2701 cgcgagcggc gtcactggcc ggcgtgaacg cggcaaa
//
LOCUS       DASUGA010000069         6099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1651600, whole genome shotgun sequence.
ACCESSION   DASUGA010000069 DASUGA010000000
VERSION     DASUGA010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651600"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            367..2226
                     /locus_tag="VFX21_01255"
     CDS             367..2226
                     /locus_tag="VFX21_01255"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEX5094603.1"
                     /translation="MADATGVVEDPPATERPHWSDRWFDAIQPPYVVLGLVIALWCVW
                     FVRLAALRNDRFGTFGFDLGIYDQGVWLLAHGRDPFVTIRGLELFGHHMNVMLVLLAP
                     FYRLGAGPEFLLGVQVAAQALGAVAIFLLGRDVLHSKWMGVGLATALLCNPTYQWLVW
                     EFFHPDAVAIGPLLLAYWAARQERWGWFTVAAVLALACKEDVALTMVVLGLLVGFRLQ
                     AKTGRVVLGMAVAAAGVLIPIQAGPQALLLLALCIAAGGIAIAWLYGTTDRIWTVIGL
                     GVALAAALVPIQSDDTWKLAVGGLVFVAGVAFALDRKPTWKIGTSIALVAAAWFVLAT
                     RVLIPRFNGVGPFYDSFFPPELGSSPTDMIRNASQHPSRAYEMLDDEAQTSWYWHMLA
                     PWALLPLFNLRVLALAFPMITVDVLTYFPYTRDYRYHYSALVVAGCALATVETIGAIK
                     RRTTYGQMAGTALVVLVLVTSVISTYQWGASPIAKDYEYIWPFHTDGANRARREGVAL
                     LPDDAATTASYSFIPHISHRERVYEWPVPWCNINWGVDGENLDRPADVDWLLVDRDLV
                     APEHQALLADLLADEFTVRFERDGIVLAERTSPPTFTRESQPPPFLCEPRPAN"
     gene            complement(2219..2971)
                     /locus_tag="VFX21_01260"
     CDS             complement(2219..2971)
                     /locus_tag="VFX21_01260"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5094604.1"
                     /translation="MNLRDRLVIVTGGGSGIGRATAARIAREGGIAAVMDQHRDAAEE
                     TTALVRGAGGNAHAFACDVSDDAAVRDTVAQIGALGRVQGVVTSAGIFYGPDLTLAHK
                     VPVDVFVRVLQVNLVGTFSVIAAALPAMMDSGGAIVTIASTAALRGHGYGAGYTASKG
                     GVDALTRLLAVQYGPHGVRANCICPGGVDTPMTAGTFSTDEAKARAKQRIPVGRYAQP
                     EDIADVAAFLLSDDSRYMTGQTFPVEGGALIS"
     gene            3031..3813
                     /locus_tag="VFX21_01265"
     CDS             3031..3813
                     /locus_tag="VFX21_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5094605.1"
                     /translation="MVTVDETNAMHTVAGKVVIVTGAGKGIGQGLALHLGKGGAAIVV
                     AEWKPDLMADTCAQLDALGISNLGVEVDIQQREQIEAMVAQTFERFGRVDCLINNAQT
                     FRPLSPIAEVSERDVDVFYTSGVKGTLWAMQAVHPHMQRQGWGRIVNFASSMGITGGT
                     GFAAYNASKEAVRALTRTAAREWAMDGIVVNAIAPAAATHHGPAGEQSEGYRIFIENC
                     PMRRQGDPELDIGGVAWFLCTDGCRYLTGHTFMVDGGAFMWA"
     gene            3851..4567
                     /locus_tag="VFX21_01270"
     CDS             3851..4567
                     /locus_tag="VFX21_01270"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HEX5094606.1"
                     /translation="MSDWPSDAWQQPPTAPPPSAAPPWAAQQPGWGPPPYDAGSYSGP
                     PSNNGFAIASLVAGCAQLIFCGIGAILAIVFGHVARGQIKRSNGTQTGRGLATAGLVL
                     GYIGLALTVLGVVAVIVVLFAFSDDITRHDMREDARAFIRSAQFEGFGSATGARDADA
                     LRETYLDDRDLTFDFDGDMYLADGTSIVTATDADWERNGWRFEMRRESPAGDVYVCAT
                     VPENVGDKVVVQNGPCLTGS"
     gene            complement(4572..5387)
                     /locus_tag="VFX21_01275"
     CDS             complement(4572..5387)
                     /locus_tag="VFX21_01275"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX5094607.1"
                     /translation="MTSRVSVSAISTYRLSLAEDLAFWAAHGIDHVGVSVAKLEAHGW
                     GDGVARVADAVGNGLRVGNLIGLGPFHLAAPEQWDAQRDRLVRALDAAVAFGAGCLVF
                     TTGPSTPLTWEEAADALEEAIRPVLAAARARSIPFAIEHTNSLRIDVGFVHTLRDVID
                     LARRLDTGVCMEVNACWAERDLLGTIRRGVDRIAVVQLSDFRVGTLSTPNRLVPGDGD
                     IPLRRIVGALLDAGYEGAFDLELVGPHIDEEGYDSAVPRAVAAVDALLAELSA"
     gene            complement(5390..>6099)
                     /locus_tag="VFX21_01280"
     CDS             complement(5390..>6099)
                     /locus_tag="VFX21_01280"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5094608.1"
                     /translation="IALRFAQEGAAVVVTGRDAGRGAATVDAITAAGGTAHFVAADLH
                     DEAECQRLVDETVTRLGGCTVLVNNAASGDATDGAVAALTTEAWESILRVDLTAPFWL
                     CRAAIPAMQRAGHGAIVNISSRQGERASPGFAAYIAAKGGLNALTRSIAVDYAREDIR
                     CNTISPGYVLNERRDADITAERRARYEGMHLTRLGTAADVASAAVYLAGAESGFLTGI
                     NLQLDGGSSIARGLTLG"
BASE COUNT          880 a         2135 c         2136 g          948 t
ORIGIN      
        1 aggtgctgcg cgtcggacgt gtgctcgaac cactcctgcg cggtctcgtc gatctcggcg
       61 cgggtctgcg cgatctggtc ctgcgtgctc tgcgcgcccg ccgacaccgg gaaacatgcg
      121 gacgcgacgc cgatagcgag cgcgaggaca ctcgcgcggg gcaacgctcg acggtgactt
      181 cccgccactc cgccgagact gcgaccggtg cgcaccgaaa ggtgccgggg ccgaagcacg
      241 gggggaaacc ctaacgaagg gtagcgggag cgtcgaacca actgaccacg cagcgtggga
      301 tttttgtcgc gggtcgggac aaccccctgg tccggcggtc gatgaaatca atgcgaaaat
      361 cggccgatgg cggacgcgac cggggtggtc gaggaccccc cagcaaccga gcgcccgcac
      421 tggtccgatc gttggttcga cgcgatccag cctccgtacg tcgtgctcgg gctcgtgatc
      481 gccctgtggt gcgtgtggtt cgtgcgactg gcggcgttgc gcaacgaccg cttcggcacg
      541 ttcgggttcg acctcgggat ctacgaccaa ggggtctggt tgctcgccca cgggcgcgac
      601 ccgttcgtga cgatccgcgg gctcgagctc ttcggtcacc acatgaacgt gatgctcgtg
      661 ctgctcgcgc ccttctaccg gctcggcgcg ggaccggagt tcctcctcgg ggtccaagtc
      721 gcggcccagg ccctcggcgc ggtcgcgatc ttcctgctcg ggcgcgacgt cctgcactcc
      781 aagtggatgg gcgtcggtct ggccaccgcg ctgttgtgca acccgacgta ccaatggctc
      841 gtgtgggagt tcttccatcc ggacgcggtc gcgatcggcc cgctgctgct cgcgtactgg
      901 gccgcccgcc aggagcgatg gggctggttc accgtcgccg cggtcctggc gctcgcgtgc
      961 aaggaagacg tcgcgctgac gatggtcgtc ctcggcctcc tcgtcgggtt ccggctgcaa
     1021 gccaagacgg ggcgcgtcgt gctcggcatg gcggtcgcgg ccgcgggcgt gctcatcccg
     1081 atccaggccg gcccgcaggc cctgttgctg ctcgccctgt gtatcgcggc cggcggcatc
     1141 gcgatcgcgt ggctctacgg cacgaccgac cggatctgga cggtgatcgg tctcggggtc
     1201 gcgttggcgg ccgcgctcgt gcccatccag tcggacgaca cgtggaagtt ggccgtgggc
     1261 gggctcgtct tcgtcgccgg tgtcgcgttc gcgctcgacc ggaagccgac gtggaagatc
     1321 ggcacgtcga tcgcgctcgt ggcggccgcg tggttcgtgc tcgcgacgcg cgtgctcatc
     1381 ccgcgcttca acggcgtggg gccattctac gacagcttct tcccgcccga gttgggttcg
     1441 tcgccgaccg acatgatccg gaacgcgtcg cagcacccgt cgcgcgcgta cgagatgctc
     1501 gacgacgagg cgcagaccag ttggtactgg cacatgctcg cgccgtgggc tctgctgccg
     1561 ttgttcaacc tgcgcgtgct cgcgctcgcg ttcccgatga tcacggtcga cgtgttgacg
     1621 tacttcccgt acacgcgcga ctaccgctac cactactccg cgctcgtggt cgcggggtgt
     1681 gcgctcgcga cggtcgagac catcggcgcg atcaaacggc ggacgactta cggccaaatg
     1741 gccgggaccg ctctcgtcgt gctcgtgctg gtcacgtcgg tgatcagcac ctaccaatgg
     1801 ggcgcgtcgc cgatcgccaa ggactacgag tacatctggc cgttccacac cgacggtgcg
     1861 aaccgggcgc ggcgcgaagg tgtcgccttg ctccccgacg acgcagcgac caccgcgtcg
     1921 tacagcttca tcccgcacat ctcgcaccgc gaacgcgtct acgagtggcc ggtcccgtgg
     1981 tgcaacatca actggggtgt cgacggcgag aacctcgacc gccccgccga cgtcgactgg
     2041 ctgctcgtcg accgtgatct cgtcgcgccc gagcaccagg cgctgctcgc cgacctcctc
     2101 gcggacgagt tcacggtccg cttcgagcgc gacggcatcg tgctcgccga gcgcacgtcg
     2161 ccaccgacgt tcacgcgcga gtcgcagccg ccgccgttcc tgtgcgagcc ccggcccgct
     2221 aactgatgag cgcgccgccc tcgacgggga acgtctggcc ggtcatgtac cgcgagtcgt
     2281 cggagagcaa gaacgcggcg acatcggcga tgtcttcggg ttgcgcgtag cgcccgacgg
     2341 gaatgcgctg cttcgcgcgc gccttggcct cgtcggtcga gaacgtgccc gcggtcatcg
     2401 gcgtgtcgac cccgcccgga cagatgcagt tcgcacgcac gccgtgcggt ccgtactgca
     2461 cggcgagcaa gcgggtgagc gcgtcaacac cacctttgct cgcggtgtac cccgcgccgt
     2521 atccgtggcc gcgcagcgcc gcggtcgacg cgatcgtcac gatcgcgccg ccgctgtcca
     2581 tcatcgccgg cagcgccgcc gcgatcaccg agaacgtccc gaccaggttc acctggagca
     2641 cgcgcacgaa gacgtcgacc ggcaccttgt gcgcaagtgt gaggtcgggg ccgtagaaga
     2701 tccccgcgct cgtcacgacg ccttgcaccc gtccgagcgc gccgatctgc gcgaccgtgt
     2761 ctcgaaccgc ggcgtcgtca ctcacatcgc aggcgaacgc gtgggcgttg ccgccggcgc
     2821 cgcgcacgag ggccgtggtc tcctccgccg cgtcgcggtg ttgatccatg accgccgcga
     2881 tgccgccttc gcgggcgatc cgggcggcgg tcgcgcggcc gatgccgctc ccgccgccgg
     2941 tgacgatgac gagccggtca cgcaggttca caaggcggca acgtacgtgc tacgcaacct
     3001 gacgcgcacg tcagcgagcc gggaggacct gtggtcaccg tcgacgaaac caacgccatg
     3061 cacacggtcg cgggcaaggt cgtgatcgtg accggtgccg ggaagggcat cggtcagggc
     3121 ctcgcgctgc acctgggcaa gggcggcgcg gccatcgtcg tcgccgagtg gaagcccgac
     3181 ctcatggccg acacgtgcgc gcagctcgac gcgctcggca tctccaacct cggcgtcgaa
     3241 gtcgacatcc agcaacggga gcagatcgag gcaatggtgg cgcagacctt cgagcggttc
     3301 ggacgggtcg actgcctgat caacaacgcg cagacgttcc ggccgttgtc gccgatcgcc
     3361 gaggtgtcgg agcgcgacgt cgacgtcttc tacacctcgg gcgtcaaggg cacgctgtgg
     3421 gcgatgcagg cggtgcaccc gcacatgcaa cgccagggct ggggccggat cgtcaacttc
     3481 gcgtcgtcga tgggcatcac cggcggcacc ggcttcgcgg cgtacaacgc gtcgaaggaa
     3541 gcggtccgcg cgctgacgcg caccgcggcg cgcgagtggg cgatggacgg catcgtcgtc
     3601 aacgcgatcg cgcccgcggc cgcgacgcac cacggccccg cgggtgaaca gagcgagggc
     3661 taccgcatct tcatcgagaa ctgcccgatg cgccgccaag gcgacccgga gctcgacatc
     3721 ggcggggtcg cgtggttcct gtgcaccgac ggttgccggt atttgaccgg ccatacgttc
     3781 atggtcgacg gcggcgcgtt catgtgggca tgaggacgcc cgcgcgcgca cgccggtagc
     3841 gtcgcgcccg atgagcgact ggccctccga cgcatggcag cagccgccga ctgccccacc
     3901 accgtcggcc gcgccaccct gggccgcgca acagcccgga tggggcccac ctccctacga
     3961 cgcgggttcc tacagcgggc cgccgtcgaa caacggcttc gcgatcgcgt cgctcgtcgc
     4021 gggctgcgcg cagctcatct tctgcggcat cggcgcgatc ctcgccatcg tcttcggtca
     4081 cgtcgcgcgc ggccagatca aacgatcgaa cggcacgcag accggacgcg gactcgcgac
     4141 cgccggattg gtgctcggtt acatcggcct cgcactcacg gtgctcggcg tcgtcgcagt
     4201 catcgtcgtc ttgttcgcgt tctccgacga catcacgcgc cacgacatgc gcgaggacgc
     4261 gcgggccttc atccggagcg cgcagttcga agggttcggc tctgcgaccg gagcccgcga
     4321 cgccgacgcg ctccgtgaga cgtacctcga cgatcgcgat ctcacgttcg acttcgacgg
     4381 cgacatgtac ctggccgacg gcacgtcgat cgtgacggcg accgacgccg attgggagcg
     4441 caacggctgg cgcttcgaga tgcgccgcga gtcgccggcg ggcgacgtat acgtgtgcgc
     4501 gaccgtgccg gagaacgtcg gcgacaaggt ggtcgtgcag aacggcccct gcctgacggg
     4561 ctcctagtgc gctacgcgct gagctcggcg agcaacgcgt cgaccgcggc gaccgcacgc
     4621 ggcactgcac tgtcgtagcc ctcttcgtcg atgtgcgggc cgacgagctc caggtcgaac
     4681 gcgccttcgt agcccgcgtc gaggagcgcg ccgacgatgc ggcgcaacgg gatgtcgccg
     4741 tcgccgggca cgagccggtt cggggtgctc agcgtgccga cgcgaaagtc gctgagctgg
     4801 acgacagcga tgcgatcgac gcccctgcgg atggtgccga ggaggtcgcg ttcggcccag
     4861 cacgcgttga cctccatgca cacgccggtg tcgagccggc gggccaggtc gatcacgtcg
     4921 cgtagcgtgt gcacgaagcc gacgtcgatg cgcagtgagt tggtgtgctc gatcgcgaac
     4981 gggatcgagc gcgcccgcgc ggcggcgagc accggccgga tcgcctcctc cagcgcatcc
     5041 gccgcttcct cccacgtgag cggcgtcgac ggtccggtcg tgaacacgag acagccggcg
     5101 ccgaatgcga ccgcggcgtc gagcgcgcgc acgagccggt cgcgctgcgc gtcccattgc
     5161 tccggtgcgg cgaggtggaa cggaccgagc ccgatcaggt tgccgacccg caggccgttg
     5221 ccgaccgcgt cggcgacgcg cgcgacgccg tcgccccaac cgtgggcctc gagcttcgcg
     5281 accgacacac cgacgtggtc gatgccgtgt gcggcccaga acgcgaggtc ttcggcgagc
     5341 gacaaccggt aggtcgagat cgcgctgacg gagactcgcg acgtcacggc tatccgagcg
     5401 tcaggccgcg ggcgatgctg ctgccgccgt cgagttgcag gttgatgccg gtgaggaatc
     5461 ccgattccgc gccggccagg tagacggccg cgctcgcgac gtcggccgcg gtgccgaggc
     5521 gcgtgaggtg catgccttcg tagcgggccc gccgctccgc tgtgatgtcc gcgtcgcgcc
     5581 gctcgttgag cacgtaaccg gggctgatcg tgttgcagcg gatgtcctcg cgcgcgtaat
     5641 cgaccgcgat cgagcgcgtg agtgcgttga gtccgccctt ggccgcgatg tacgcggcga
     5701 acccggggct cgcacgttcg ccttggcgcg aggagatgtt cacgatcgcg ccgtgacccg
     5761 cgcgctgcat ggccgggatc gcggcgcgac acagccagaa cggtgcggtc aggtcgacgc
     5821 gcaggatcga ctcccacgcc tcggtcgtca gcgcggcgac ggcgccatcg gtcgcatcac
     5881 cgctcgcggc gttgttgacg agcacggtgc agccgccgag gcgcgtgacg gtctcgtcga
     5941 cgaggcgttg gcactcggct tcgtcgtgca gatcggcggc aacgaagtgc gcggtcccgc
     6001 ccgcggccgt gatcgcgtcg acggtcgcgg cgccgcgacc cgcgtcgcgg ccggtgacga
     6061 cgacggccgc gccctcctgc gcgaaccgga gcgcgatcg
//
LOCUS       DASUGA010000070         3257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2066907, whole genome shotgun sequence.
ACCESSION   DASUGA010000070 DASUGA010000000
VERSION     DASUGA010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2066907"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VFX21_01285"
     CDS             complement(<1..876)
                     /locus_tag="VFX21_01285"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEX5094609.1"
                     /translation="MRVLVVNAGSSSLKLRILGDDDRIIAASDAESLTQFVNGSPAVD
                     AVGHRVVHGGTEFTDPAEIDEDVLRRLDALAPLAPLHQPRAIELIREARSLVPGVPHV
                     ACFDTAFHATIPDFATTYALPSAWRERWPVRKFGFHGLSHAYAARRAAEDLGRTPGTV
                     RVVTCHLGAGASLCAVCDGRSVDTTMGMTPLDGVVMATRAGAVDPGLLVWLQQEAGMS
                     VDELEIGLEDESGLLGLAGTSNMREVLARRAAGDERARLAFDVYVHRLRQGIAAMAVS
                     VAPLDALVFTGGVGEH"
     gene            complement(881..3241)
                     /locus_tag="VFX21_01290"
     CDS             complement(881..3241)
                     /locus_tag="VFX21_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017321790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEX5094610.1"
                     /translation="MDRPLSDELLEKMNAYWRAANYLSVGQIYLFANPLLEEPLTLEH
                     IKPRLLGHWGTTPGLNIVYTHLNRCIARDDLDVVYIAGPGHGGPAIHANCWLEGTYSE
                     IYRGIPRDARGMEHLFRQFSFPGGVPSHVAPEIPGSIHEGGELGYSLSHAFGAAFDNP
                     ELIVACVIGDGEAETGPLATSWHGNKFLDPVGDGAVLPILHLNGYKIANPTISARVGD
                     DELSLLLAGCGWTPYLVAGDDPELVHQQLAATLDRVLDDIRKFQRRAREHGDRSRPRW
                     PMIVLRTPKGWTGPHEVDGVQIEGTWRAHQVPLPVSRAHPRHLADLERWMRSYSPDEL
                     FDDDGAPRAIVAGWLPRGDRRIGANPHANGGLLLRDLQLPDFRDYGVRVDAPGATDAE
                     ATRVLGTFLRDVMAANMDTANFRLFGPDEVASNRLDDVYEVTDKVWQGEVLLVDEHLA
                     RHGRVMEILSEHTCQGWLEGYLLTGRHGLFTCYEAFIHIVDSMFNQHAKWLETAQTLE
                     WRRSIASLNYLITSHVWRQDHNGFTHQDPGFLDVVVNKKPSVVRVYLPPDANCLLSVG
                     DHCLRTRDYVNVIVAGKQSQPTWLDIEDASLHCARGLGIWDWASNDEGGTPDVVMACA
                     GDVPTLETLAAVSMLREYLPELKVRVVNVVDLMRLVPETEHPHGLSDREFDAIFTTTA
                     PIIFAFHAYPYLIHRLTYRRNGHHNLHVRGYKEEGTTTTPFDMVVMNDLDRFHLVMDV
                     IDRVPGLGARAAHVRQHMVDQRARHHRYVREAGEDMPEITGWTWPG"
BASE COUNT          523 a         1114 c         1111 g          509 t
ORIGIN      
        1 gtgctcgccg acgccgccgg tgaacaccag cgcgtccaac ggtgcgacac tgaccgccat
       61 cgcggcgatg ccctgccgca accggtgcac atagacgtcg aacgcgaggc gggcgcgctc
      121 gtcgccggcc gctcgtcgcg cgagcacttc ccgcatgttg ctcgtccctg ccaggccgag
      181 cagcccggac tcgtcttcca gcccgatctc cagctcgtcg accgacatcc cggcttcctg
      241 ctgcagccag acgagcagtc ccgggtcgac cgcacccgcg cgcgtcgcca tcaccacacc
      301 gtcgagcggc gtcatgccca tcgtggtgtc gaccgagcgg ccgtcgcaga ccgcgcacag
      361 cgacgcaccc gcgccgaggt ggcaggtgac gacgcgcacg gtccccggcg tgcgcccgag
      421 gtcctcggca gcgcgccgcg ccgcgtaggc gtgcgacagg ccgtggaagc cgaacttccg
      481 taccggccaa cgctcgcgcc acgcgctcgg gagcgcgtac gtcgtcgcga agtccgggat
      541 cgtcgcatgg aacgcagtgt cgaagcaggc aacgtgcggg acgccgggca cgagcgatcg
      601 cgcctcacgg atcaactcga tcgcgcgcgg ctggtgcaat ggggcgagtg gcgccagcgc
      661 gtcgagccgg cggaggacgt cttcatcgat ctcggcggga tcggtgaact ccgtaccgcc
      721 gtgcacgacg cgatgcccaa ccgcgtcgac cgccggggaa ccgttgacga actgagtgag
      781 cgactcggcg tccgacgcgg cgatgatgcg gtcgtcgtcg cccaggatac gcagcttcag
      841 gctgctggaa ccggcgttga cgacaaggac gcgcacggcc tcagcccggc cacgtccacc
      901 cggtgatctc cggcatgtcc tcccccgcct cgcgcacgta ccggtgatgc cgggcccgct
      961 ggtcgaccat gtgctggcgc acgtgcgcgg cgcgggcacc caaacccggc acgcggtcga
     1021 tgacatccat aaccaggtgg aaccggtcga ggtcgttcat cacgaccatg tcgaacggtg
     1081 tcgtcgtcgt gccctcttcc ttgtacccgc gcacgtgcag gttgtggtgg ccgttgcggc
     1141 gatacgtcag gcggtggatc aagtacggat acgcgtggaa ggcgaagatg atcggcgcag
     1201 tcgtcgtgaa gatggcgtcg aactcgcggt cggacagtcc gtgcggatgc tcggtctcgg
     1261 gcacgaggcg catgaggtcg acgacgttga ccacgcgcac cttcaactcg gggagatact
     1321 cgcgcaacat cgacaccgcc gcgagcgtct cgagcgtcgg cacgtcaccc gcgcacgcca
     1381 tgacgacatc gggcgtacca ccttcgtcgt tgctcgccca atcccagatc ccgagcccac
     1441 gcgcgcagtg caggctcgcg tcctcgatgt cgagccacgt cggctgcgac tgcttccccg
     1501 cgacgatgac gttcacgtaa tcgcgcgtcc gcaggcagtg gtcaccgacc gagagcaggc
     1561 agttcgcgtc gggcggcaag tagacacgca cgaccgacgg cttcttgttc acgacgacgt
     1621 cgaggaaccc cggatcttga tgcgtgaagc cgttgtggtc ctggcgccag acgtgcgacg
     1681 tgatcaagta gttcaacgac gcgatgctcc ggcgccattc gagcgtctgc gcagtctcga
     1741 gccacttggc gtgctggttg aacatcgaat cgacgatgtg gatgaacgcc tcgtagcacg
     1801 tgaagaggcc gtggcgcccg gtcagcaagt agccctccaa ccaaccctgg cacgtgtgct
     1861 cgctgagaat ctccatcacg cgtccgtgcc gcgcgaggtg ctcgtcgacg aggaggacct
     1921 cgccctgcca caccttgtcg gtgacttcgt acacgtcgtc gagccggttc gacgcgactt
     1981 cgtcgggccc gaagagccgg aagttcgcgg tgtccatgtt cgcggccatg acgtcgcgca
     2041 ggaacgtccc cagcacgcgc gtcgcttccg cgtcggttgc gccgggtgcg tcgacccgaa
     2101 cgccgtagtc gcggaagtcc gggagctgca ggtctcgcag gagcagaccg ccgttcgcgt
     2161 gcgggttcgc accgatgcgg cgatcgccgc gcgggagcca cccggcgacg atcgcgcgcg
     2221 gcgcgccgtc gtcgtcgaac agttcgtcgg gcgaatagga gcgcatccag cgctccaagt
     2281 cggcgaggtg acgcggatgc gcacgcgaca ccggcaacgg cacctggtgc gcgcgccacg
     2341 tgccctcgat ctgcacacca tcgacctcgt gcgggcccgt ccagcccttc ggcgtccgca
     2401 gcacgatcat cggccagcgc ggccggctcc ggtcgccgtg ctcgcgcgcg cgtcgctgga
     2461 acttgcggat gtcgtcgagc acccggtcga gcgttgccgc gagctgctgg tggacgagct
     2521 cggggtcgtc cccggccacg agatacggcg tccagccgca gcccgcgagc aagaggctca
     2581 gctcgtcgtc accgacgcgc gcgctgatcg tggggttcgc gatcttgtac ccgttgaggt
     2641 ggaggatggg cagcaccgcg ccgtcgccga cgggatcgag gaacttgttg ccgtgccagc
     2701 tcgtcgcgag cgggcccgtc tcggcttcgc cgtcgccgat gacgcacgcg acgatcagct
     2761 cggggttgtc gaacgcggca ccgaacgcgt gcgagagcga gtagccgagc tcgccgcctt
     2821 cgtggatcga accgggaatc tccggcgcga catgcgacgg cacgccaccc gggaacgaga
     2881 actgacggaa caagtgctcc atgccgcgcg catcacgcgg aatcccgcgg tagatctccg
     2941 agtacgtgcc ctcgagccag cagttcgcgt ggatcgccgg accgccgtgc ccggggccgg
     3001 cgatgtagac gacgtcgagg tcgtcgcgcg cgatgcatcg gttgaggtgc gtgtagacga
     3061 tgttcagacc gggcgtcgtg ccccagtggc cgagcagtcg cggcttgatg tgctcgagcg
     3121 tgagcggctc ctcgaggagc gggttcgcga agaggtagat ctggccgacc gagaggtagt
     3181 tcgcggcgcg ccagtaggcg ttcatctttt cgagcagctc gtccgaaagc ggtctatcca
     3241 tgattcctcg cggctct
//
LOCUS       DASUGA010000071         3468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_955644, whole genome shotgun sequence.
ACCESSION   DASUGA010000071 DASUGA010000000
VERSION     DASUGA010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955644"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VFX21_01295"
     CDS             complement(<1..890)
                     /locus_tag="VFX21_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094611.1"
                     /translation="MLDDVYSDDEYRRILEVIKREGPWGTIISHHFTTVDEMIATVTG
                     VVPPDHGLTLDDVAGPHFRGFFGQNSVCFYPEIEDVFYSARFLECVKSYWNAQYAKPT
                     LMLFNICGPHDTGLAPHLDAVSFRGVTINNTPVWLQNIMGKSGLFTDYLVKMAQVITW
                     WYRGEPGGFTYWPRGPFEAPERLTAPMWNKGVVVQNEMMFHRGDPVGPIEDRPIEGMK
                     ARSVFGYDPADDSWAVTTDGAVIKRVRPEQIRLLVHWSAEVYADMTEVKKVMDHTDDL
                     THQQVVDTLLADMRSRGVKVA"
     gene            1043..1495
                     /locus_tag="VFX21_01300"
     CDS             1043..1495
                     /locus_tag="VFX21_01300"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5094612.1"
                     /translation="MIDVAAVMNVLAAWWFNYDEGNFDVLRGLLTEDVHFTCRTDTDT
                     VAWAEFARADHRGRDTVMHWQTEHRRASPYPLRHHGTNVHVVEQRGEEASFASYIHVT
                     QVVNEMPAAIPGGIVTGVVREADDGVRIAKMDVVLDTMTSDVFSTVKA"
     gene            1528..2325
                     /locus_tag="VFX21_01305"
     CDS             1528..2325
                     /locus_tag="VFX21_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5094613.1"
                     /translation="MLVTGASRGIGAAAAVRFAAEGADVAIVARTLDQHDHLSGSLRE
                     TADRVRAHGGAVCVITADIANDDERSRIVPTAVEALGGSIDVLINNAAAAMYAPVAEF
                     PLKRQRLTFDVNVIAPISLAQAVLPDMLARGEGWIVNVSSATARRTPNSTMGVYGASK
                     AALNRLTQALATELAGTGVRANTIEPRAAVLTEGAAALVGDTVHADQVETLEEMVEAL
                     VAVASCSTDVSGCNLVSLDVLRDWNLVVHELDGSTRDQPANTHQEHV"
     gene            2322..3098
                     /locus_tag="VFX21_01310"
     CDS             2322..3098
                     /locus_tag="VFX21_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5094614.1"
                     /translation="MTDARQLFDLTGKVAVVTGSTKGIGRAMATGLAQAGASVVVSSR
                     KQDLCEKVANEVAEETGRDTLGLACHVGDWDAIPEFVDAVVERFGKIDVLVNNAGITP
                     AREGPATIELAIWRKIFSVNVEGPLRLGQCVAPIMRDNGGGSIVNIASMAAYSGGPLV
                     CAYGASKAALVNLTKSQAQEWMPWNVRVNVLSPGPFESEMTSGAERAAPGFKDMIAAG
                     TLMKRVADPVEIVGPVVYLASDASSFVTGDDISVSGGMQK"
     gene            3149..>3468
                     /locus_tag="VFX21_01315"
     CDS             3149..>3468
                     /locus_tag="VFX21_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5094615.1"
                     /translation="MTACSRDKETTESSDAESATTASEGQSSNNAQGLDAGAFGDLGV
                     ICQPGDGATASSSTSDPGVTADSVQISTFSDPGFSGRLGLNQELFDTAEAFTKWCNEH
                     GGING"
BASE COUNT          619 a         1111 c         1160 g          578 t
ORIGIN      
        1 gccaccttca cgccccgcga ccgcatatcg gccaacagcg tgtcgacgac ctgctggtgt
       61 gtgaggtcgt cggtgtggtc catgaccttc ttgacctccg tcatgtcggc gtagacctcg
      121 gcgctccagt gcacgagcag ccggatttgc tccggacgaa cacgcttgat gaccgcgcca
      181 tcggtcgtga ccgcccacga atcatcggca gggtcgtagc cgaacaccga gcgcgccttc
      241 atgccctcga tcggacgatc ttcgatcggg ccgaccggat cgcctcggtg gaacatcatc
      301 tcgttctgca cgacgacgcc cttgttccac atcggtgcgg tcagccgctc cggcgcctcg
      361 aagggcccgc gcggccaata cgtgaacccg cccggttcgc cgcggtacca ccacgtgatc
      421 acctgcgcca tcttcaccag gtagtcggtg aagaggcccg acttgcccat gatgttctgc
      481 agccagaccg gcgtgttgtt gatcgtgacg cctcgaaacg acactgcgtc gagatgcggc
      541 gcgagccccg tgtcgtgtgg tccgcagatg ttgaacaaca tcagcgtcgg cttggcgtac
      601 tgcgcgttcc aatacgactt cacacactcg aggaaccgag cgctgtagaa cacgtcctcg
      661 atctcggggt agaagcagac ggagttctgc ccgaagaaac cgcggaagtg gggccccgca
      721 acgtcgtcca gtgtcaaccc atggtcgggc gggacgacgc ccgtcacggt tgcgatcatc
      781 tcgtccacag tcgtgaagtg gtgcgagatg atcgtccccc acgggccttc ccgcttgatc
      841 acctcgagga tgcggcggta ctcgtcgtcg gagtacacgt cgtcgagcac gcgaggcgga
      901 gccgccggcg taaggacttg ggcgagggac gacggcgtgg aatcgctcac ggccaacctc
      961 cggggtcggg cgccccttgc gggcggcggg tatgaagata tagtaacgct gttctctttt
     1021 ccagccgtgc cggggggcgc ccgtgatcga cgtcgccgca gtcatgaacg tgctggccgc
     1081 gtggtggttc aactacgacg aaggcaactt cgacgtgctc cgcggcctcc tcaccgaaga
     1141 cgtgcacttc acgtgccgca ccgatacgga cacggtggcg tgggcggagt tcgcccgtgc
     1201 cgaccaccgg ggtcgcgaca cggtgatgca ctggcaaacg gagcaccgcc gcgccagccc
     1261 gtatccactt cgtcaccacg gcacgaatgt gcacgtggtc gaacaacgcg gcgaggaagc
     1321 gtcctttgcg tcgtacatcc acgtcacgca ggttgtgaac gagatgccgg cggcgatccc
     1381 aggcgggatt gtcaccggtg tcgtacgcga ggccgatgac ggcgtgcgga tcgcgaagat
     1441 ggatgtcgtg ctcgacacca tgacatctga cgtgttctcg acagtgaagg catgagtcgg
     1501 gcgcaaccgc gctttcatgg tcggcgcgtg ctggtgaccg gcgcgagccg cggcatcggc
     1561 gcggccgccg cggtgcggtt cgccgccgaa ggtgctgacg ttgccatcgt tgcgcgtacc
     1621 ctcgaccagc atgatcacct gtcgggcagc ctccgggaga ccgccgatcg cgtgcgggcg
     1681 cacggcggcg cggtctgcgt gatcacagcc gatatagcga atgacgacga acgttcgcga
     1741 atcgtgccga cggctgtcga agcgctcggc gggtccatcg acgtgctgat caacaacgcg
     1801 gcggccgcga tgtatgcgcc ggtcgctgag ttcccactca agcggcagcg tctcacgttc
     1861 gacgtgaacg tgattgcgcc aatctcgctc gcccaggctg tgctgcccga catgcttgcc
     1921 cgtggcgaag ggtggatcgt taacgtctcg tcggcaacgg cgcgtcgcac gcccaactcg
     1981 accatgggcg tatacggcgc gtccaaggcg gcgttgaacc ggctgacgca agccctggca
     2041 accgaactcg cgggaaccgg cgtacgcgcc aacaccatcg agccacgagc ggcggtcctg
     2101 accgagggtg cggcggcact cgtcggcgac acggtccacg ccgaccaagt ggaaacgctc
     2161 gaggagatgg tggaagccct cgtcgccgtg gccagctgct ccaccgacgt gtcgggctgc
     2221 aacctcgtca gtctcgacgt gttgcgcgat tggaatctcg tcgtgcacga gctcgacgga
     2281 agcactcgtg accagcctgc caacacccat caggagcacg tatgaccgac gcacgccaac
     2341 tctttgacct gacgggaaag gtcgcggtcg tgaccgggtc gaccaaaggg atcggccgcg
     2401 cgatggcgac cggtcttgcg caggcgggcg cctcggtcgt ggtgagcagt cgcaagcagg
     2461 atctgtgcga gaaggtggcg aacgaagtcg ccgaggaaac cgggcgcgac acgctcggac
     2521 tcgcgtgcca cgtcggcgac tgggatgcga ttccggagtt cgtcgacgcc gtcgtcgaac
     2581 gcttcgggaa gatcgacgtt ctcgtgaaca acgccgggat cacgcctgca cgcgaagggc
     2641 cggcgacgat cgaactggcg atctggcgca agatcttctc ggtcaacgtc gagggaccac
     2701 tgcgcttggg ccagtgcgtc gcgccgatca tgcgcgacaa cggtggtgga agcatcgtca
     2761 acatcgcgtc gatggccgcg tactccggcg gcccgctggt ctgcgcctac ggcgcgtcga
     2821 aggctgcgct tgtgaacctc accaagagcc aggcgcagga atggatgccg tggaacgtgc
     2881 gcgtgaacgt gttgtcacct ggtcccttcg aaagtgagat gaccagtggc gccgagcgcg
     2941 ccgccccggg cttcaaggac atgatcgcgg ccggcacgtt gatgaagcgg gtcgcggacc
     3001 cggtcgagat cgtcggcccg gtggtgtacc tcgcgagcga cgcttcgtcg ttcgtcaccg
     3061 gcgacgacat ctctgtctcg ggaggaatgc agaaatgaga cgccggatcg tcgcgcgggg
     3121 ggtcgcgctt ctcctcgtta cagtggccgt gaccgcgtgc agccgcgaca aggaaacgac
     3181 cgagtcgtcg gacgccgaga gcgcgacgac cgcgtccgaa gggcaaagct cgaacaacgc
     3241 gcagggcctg gacgccggcg cgttcggaga cctcggcgtc atctgccaac ccggcgacgg
     3301 ggcgaccgcg agttcgagta ccagcgaccc tggcgttacg gccgacagcg tgcagatcag
     3361 cacgttctcc gatcccggat tctccggtcg cctcggactg aatcaggagc tcttcgatac
     3421 cgcggaggcg ttcaccaagt ggtgcaacga gcacggcggt atcaacgg
//
LOCUS       DASUGA010000072         2247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_815551, whole genome shotgun sequence.
ACCESSION   DASUGA010000072 DASUGA010000000
VERSION     DASUGA010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815551"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="VFX21_01320"
     CDS             <1..1113
                     /locus_tag="VFX21_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX5094616.1"
                     /translation="IIATNPIVSFPNLGVLTQALEGLEFLVVQDGFHPTPTSEHADLV
                     LPGAIWGEKEGTYTNSERRVSKVNRAVPPPEEARADFDIFLGLAGALGVRDELFNGWT
                     KPADAFEEWKRVSAGRLCDYSGMTYKAIEQHGGLQWPVPAGALPDDEPRRLYTDGKFR
                     TEDGRARLIPCTWEPFPEQPNTEFPFVLNTGRTVEHWHTRTKTAKVPILERLSPSAWV
                     EMNPADARALRLKPQARVDIVSRRGRVQSVELRVTETIAPGQLFTPFHYFEANANQVT
                     QSAFDPISREPNYKQSAVRIEPATRDPGLGSRGPRFVVAAKNRLSVARFWRGSTIPLL
                     SHREARTESRTPDPGSPIPGPGSRLPRAGSGDGGAR"
     gene            1110..>2247
                     /locus_tag="VFX21_01325"
     CDS             1110..>2247
                     /locus_tag="VFX21_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5094617.1"
                     /translation="MKRLVVVGNGMAGMACVEQILKYAPPAGDAGGAASASRAEMFRI
                     TVFGDETHTNYNRVMLSSVLAGEKNADEIVINSREWYRRNEIDLRVGVRIIDVDPVRK
                     TVIGNDGSVTPYDTLLLATGSTAWMPPIKGLDKDGVFTFRTLDDTRELLERAGPDTNA
                     VVIGGGLLGLEAARGLQVQGCRVTVVHLMGTLMERQLDPAGGRFLMSKMKDLRIRVLL
                     GKSTTAILGDRRVTGVEFTGGERLDANLVVVAAGIRPNVELGRTAGLTVKRGIVVNDF
                     METSNPDIFAVGECVEHRGVCYGLVAPLFEQGKVLAATITGRRGPTYEGTVQAAKLKI
                     VGVEMFSAGEIDDDGSEPVRYEDPTLGVYKKVAVRNGKLAGVILV"
BASE COUNT          403 a          719 c          767 g          358 t
ORIGIN      
        1 atcatcgcga ccaatccgat cgtctcgttc ccgaacctcg gcgtgctcac gcaggcgctc
       61 gaggggctcg agttcctcgt cgtgcaggac ggcttccatc cgactcccac gagcgagcat
      121 gccgacctcg tcctgccggg agcgatctgg ggcgagaagg aagggaccta caccaactcc
      181 gagcggcgcg tgagcaaggt gaatcgcgcg gtgccgccgc cagaggaagc gcgcgccgac
      241 ttcgacatct ttctcggcct cgccggtgcg ctcggcgtcc gcgacgagct gttcaacggg
      301 tggacgaagc ccgccgacgc gttcgaagaa tggaagcgcg tgtctgcggg ccgcctgtgc
      361 gactactccg gtatgaccta caaggcgatc gagcagcacg gcggactgca gtggccggtg
      421 ccggcgggag cgttgcccga tgacgagccg cgccgtctat acaccgacgg aaagtttcgc
      481 accgaggatg gccgcgccag gctgatccca tgcacatggg aaccgtttcc ggagcagccg
      541 aacaccgagt tcccattcgt cctcaacacc ggtcgcacgg tcgagcactg gcacacgcgc
      601 acgaagacgg ccaaggtgcc gattctcgaa cggctgtcgc cgagcgcgtg ggtcgagatg
      661 aacccagccg atgcacgtgc gcttcggctg aagccacagg cgcgcgtcga tatcgtgtcg
      721 cggcgcgggc gcgtgcagag cgtggagctc cgcgtcaccg aaacgatcgc gccggggcaa
      781 ctgttcaccc cgtttcacta cttcgaggcg aacgcgaatc aggtgacgca gagcgcgttc
      841 gacccgatct cgcgcgagcc caactacaag caatccgccg tgcgcatcga gccggctacc
      901 cgggatccgg gactcgggag ccgcggcccg aggttcgtcg tggctgcaaa gaaccggctg
      961 tcggtcgccc ggttctggcg tgggtcgacc attccgctgc tttctcaccg ggaggcacgc
     1021 accgaatcga gaaccccgga cccagggtcc cccatcccgg gtcccggatc ccggctcccg
     1081 cgcgctggat ccggcgatgg aggtgcgcga tgaagcggct cgtcgtcgtc ggcaatggca
     1141 tggccgggat ggcctgcgtc gaacagatcc tcaagtacgc accgcccgcc ggcgatgccg
     1201 ggggcgccgc gagcgccagt cgagcggaga tgttccgcat caccgtcttc ggtgacgaga
     1261 cgcacaccaa ctacaaccga gtgatgttgt cgtccgtgct cgccggcgag aagaacgctg
     1321 acgagatcgt catcaactcg cgcgagtggt atcgccgcaa cgagatcgac cttcgcgttg
     1381 gcgttcgcat catcgacgtc gatccggtgc ggaagaccgt catcggcaac gacgggagcg
     1441 tgacaccgta cgacacgttg ctgctcgcca ccggcagcac cgcgtggatg ccacccatca
     1501 aaggactcga caaggacggc gtcttcacgt tccgcacgct cgacgacacg cgcgagttgc
     1561 tggagcgagc gggtcccgat acgaacgcag tcgtcatcgg cggtggactg ctgggcctcg
     1621 aggccgcgcg cggtctgcag gtgcaggggt gccgcgtgac cgtcgttcac ctgatgggaa
     1681 cgctgatgga gcggcaactc gatcctgcag gcggacgctt cctcatgtcg aagatgaagg
     1741 acctccgcat ccgcgttctg ctggggaaga gcacgacggc gatcctgggc gatcggcgtg
     1801 tcacgggcgt ggagttcacc ggcggcgaaa gactggatgc gaacctcgtc gtcgttgcgg
     1861 cagggatccg gccgaatgtg gagcttgggc gaacggccgg cctgaccgtg aagcgcggga
     1921 tcgtcgtgaa cgatttcatg gagacgtcga accccgacat cttcgcggtt ggcgagtgtg
     1981 tcgagcatcg aggtgtgtgt tacgggttgg tcgcacccct gttcgagcag ggcaaggtgc
     2041 tcgcggccac aatcaccggc cgccgcggtc ccacctacga gggcaccgtg caggcggcaa
     2101 agctgaagat cgtcggcgtc gagatgttct cggcgggcga gatcgacgac gacggctcgg
     2161 agccggtcag gtacgaagac ccgacgctcg gcgtgtacaa gaaggtggcc gttcgcaacg
     2221 gcaagcttgc cggcgtgatt ctggtcg
//
LOCUS       DASUGA010000073         2181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1093467, whole genome shotgun sequence.
ACCESSION   DASUGA010000073 DASUGA010000000
VERSION     DASUGA010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093467"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..1388)
                     /locus_tag="VFX21_01330"
     CDS             complement(228..1388)
                     /locus_tag="VFX21_01330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013987393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX5094618.1"
                     /translation="MATAVIVDAVRSPLGRRNGDLKDIHPVDLVAQVMRQLVERNNLD
                     PALIEDVIMGCVMQVGEQGINVGRNAAFAAGFPESVPGTTIDRQCGSSQQAAHFAAQG
                     VIAGAYDVVIAAGVENMTRVPMGASVAGGAHGFPFGPMMTARYPNLVPQGISAELISE
                     KWNISREDNDRFSVESHLRAARAREEGRFDREIVPITVTGEDGETTVSQDSGIRPDSS
                     VEKLANLKPAFKEDGVITAGNSSQISDGASALLIMSEEKANALGLKPRARFHTFALAG
                     VDPVLMLTGPIPATTKVLEKANMKIEQMDIVEINEAFAPVVLAWEKEHHPDMSKVNVN
                     GGAIALGHPLGASGARLMATLLNELERTGGRYGLQTMCEGGGMANATIIERL"
     gene            complement(1425..>2181)
                     /locus_tag="VFX21_01335"
     CDS             complement(1425..>2181)
                     /locus_tag="VFX21_01335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5094619.1"
                     /translation="AERLREADQMRSDFLALVSHELRTPLTAAKGFVDTVLYQWDRLE
                     DDRRKELLARASRNAEELTRLIDKLLAYSGLEAHPVLDVRPVALGDAIEHVREHCAPA
                     LGDHPIVVDVPDRLYARADAHAFGHLLENLLTNAAKFAPTGTPIIVSATVDGAEIAVR
                     VTDEGPGIPESEQEKVFERFYRSPAHRNASRGTGLGLTIARRAADALGGRLWVESEPG
                     YGASFVFTLPAATVSAGPGLTPTVATDRLDQPV"
BASE COUNT          329 a          735 c          761 g          356 t
ORIGIN      
        1 cggtagaact gatcgagcgt cacgtcgtcg gcgagctcga cgtcgtcggg ggcgggcggc
       61 ggttcgcccc gggcgaagcc gcggatcatc gacgcggccc acaggtagat gccgatcgcg
      121 atggcgaacg cacctacgac ccggatgatg tcccagacca tcgccgtcca ggctaggccc
      181 ggacggcgat gcgtcaggag tcgttgcgag cgaccggcgc tcgctgccta caagcgctcg
      241 atgatggtgg cgttggccat gccgccgcct tcgcacatcg tctggaggcc gtagcggccg
      301 ccggtgcgct cgagctcgtt gagcagcgtc gccatcaagc gcgcacccga cgcgccgagc
      361 gggtgcccga gtgcgatcgc gcccccgttg acgttcacct tcgacatgtc ggggtggtgt
      421 tccttctccc acgccaacac gacgggcgcg aacgcctcgt tgatctcgac gatgtccatc
      481 tgctcgatct tcatgttggc cttctcgagg accttcgtgg tcgccgggat cgggccggtc
      541 agcatcagca ccgggtcgac gccggcgagc gcgaacgtgt ggaagcgagc acgcggcttg
      601 agcccgagag cgttcgcctt ctcctccgac atgatcagca gcgcggaagc gccgtcggag
      661 atctgcgacg agttgccggc cgtgatcacg ccgtcttcct tgaacgcggg cttgaggttc
      721 gccaacttct ccacgctgga gtcggggcgg atgccgctgt cctggctcac ggtcgtctcg
      781 ccgtcttcac ccgtcaccgt gatgggcacg atctcgcggt cgaagcgacc ctcttcacgc
      841 gcgcgcgccg cccgcaggtg cgactcgacc gagaagcggt cgttgtcttc gcgcgagatg
      901 ttccacttct cggagatcag ctcggccgag atgccctgcg ggacgaggtt cgggtagcgc
      961 gcggtcatca tcggaccgaa cgggaagccg tgcgcgccac cggcaacgga cgcgcccatc
     1021 ggcacgcgcg tcatgttctc gacgcccgcc gcgatcacaa cgtcgtacgc gccggcgatc
     1081 acgccctgcg cagcgaagtg ggcagcctgc tgcgacgagc cgcactgacg gtcgatggtc
     1141 gtgccgggga ccgactcggg gaacccggcc gcgaacgcgg cgttgcggcc gacgttgatg
     1201 ccctgctcgc cgacctgcat gacgcagccc atgatcacgt cctcgatgag ggccggatcg
     1261 agattgttgc gttcgaccag ctggcgcatg acctgcgcga cgaggtcgac ggggtggatg
     1321 tccttgaggt cgccgttgcg gcgtccgaga ggtgagcgga ccgcgtcgac gatgactgcc
     1381 gtcgccatga tgggcctcca gagaaagcga gctgcgagcg agaactagac cggttggtca
     1441 agtctgtcgg tggcgacggt tggggtcaaa cccggccccg ccgacaccgt cgccgcgggc
     1501 agcgtgaaca cgaagctcgc gccgtacccg ggctcgctct ctacccaaag gcggccgccg
     1561 agcgcgtcgg ccgcccggcg ggcgatcgtg agccccagac cggttccccg cgacgcgttg
     1621 cggtgcgcgg gtgaccggta gaaacgctcg aagaccttct cttgttccga ctcggggata
     1681 cccggccctt cgtcggtgac acggacggcg atctcggcgc cgtcgacagt ggccgagacg
     1741 atgatcggcg tgccggtcgg tgcgaacttc gcggcgttcg tcagcaggtt ctcgaggaga
     1801 tgcccgaacg cgtgcgcgtc cgcgcgtgcg tacaagcggt cgggaacgtc gacgacgatc
     1861 gggtggtcgc ccaacgccgg tgcacagtgc tcgcgcacgt gctcgatcgc gtcaccgagc
     1921 gcgaccgggc gcacgtcgag caccgggtgc gcttcgagcc ccgagtacgc gagcagcttg
     1981 tcgatgaggc gcgtcagctc ctcggcgttg cggctggcgc gcgccagcag ctccttgcgc
     2041 cggtcgtcct cgagccggtc ccactggtag agcacggtgt cgacgaagcc cttcgccgcg
     2101 gtcaacggcg tgcgcagctc gtgcgagacg agcgcgagga aatcggaccg catctggtcc
     2161 gcttcgcgca accgttctgc g
//
LOCUS       DASUGA010000074         2170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1093189, whole genome shotgun sequence.
ACCESSION   DASUGA010000074 DASUGA010000000
VERSION     DASUGA010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093189"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1513
                     /locus_tag="VFX21_01340"
     CDS             <1..1513
                     /locus_tag="VFX21_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FadD3 family acyl-CoA ligase"
                     /protein_id="HEX5094620.1"
                     /translation="GNTEALVDERDRLTFAQLQDAAFGAARALVASGIQPGDRVGIWA
                     PNTTEWVVAALGIYAAGGVVIPLNTRFKGGEASYILNRAHVKLLFTVTDFLATDYVEL
                     LKDGDDVPTLETIVVIRGADAPGTISWTDLLARGRDVAQSEIDARIAALTGDTVSDIL
                     FTSGTTGRPKGAMLTHGASVRAYDAWASVVGLQHGDRYLVVNPFFHAFGLKAGILASL
                     IKGATIVPHAVFDVDQVMQRVVDESISMLPGPPTVYQSILDHPRATEFDMSSLRLGVT
                     GAAPVSVELVRRIREELKFDTIVTGYGLTEATGIATMCRHDDPPETISRTSGRAIPGV
                     EVRLVDDDNNSVPTGEPGEVVVRGYNVMKGFLDDPDATAEAIDSEGWLHTGDIAVMDD
                     GGNVTITDRKKDMFIVGGFNAYPAEIENMLSAHPSISQVAIVGVPDTRMGEVACAFVV
                     LRPNTTLTPDELIAWSRDKMANYKVPRHVDILDALPLNASGKVLKYELRNSRK"
     gene            complement(1569..>2170)
                     /locus_tag="VFX21_01345"
     CDS             complement(1569..>2170)
                     /locus_tag="VFX21_01345"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5094621.1"
                     /translation="AMLVDGVGVPRPRLDADQTGFGPTDRLYRTNDGWIQIAAVKDEH
                     WRGLCAAVGQPDLADDARFATPAARAEHRQQLEALLAPAFAGGTAVVWSRVLDDHGVP
                     NEVPVDTLAGRLALFDADNERLGLVAEYEHPVVGTMRQYGRFFDFSATPTNPDRPPPR
                     VGEDTKDILEELGYDAARMDDLKNDNVVYWPDENYSWTV"
BASE COUNT          348 a          759 c          730 g          333 t
ORIGIN      
        1 cggaaatacg gaagccctcg tcgacgagcg tgaccggctg acgtttgcgc agctccagga
       61 cgccgcgttc ggtgcggccc gggccctggt cgcatcgggc atccagcccg gcgaccgcgt
      121 cggcatctgg gccccgaaca ccacggagtg ggtcgtcgcc gcgctcggaa tctacgcggc
      181 cggcggcgtc gtcatcccgc tgaacactcg gttcaagggc ggcgaagcgt cgtacatcct
      241 caaccgggcc cacgtgaagc tgctgttcac cgtcaccgac ttcctcgcga ccgactacgt
      301 cgagctcctg aaggacggcg acgacgtccc gacgctcgag acgatcgtcg tgatccgcgg
      361 cgccgacgcg ccgggcacga tctcgtggac cgacctcctc gcgcgcggcc gcgacgtcgc
      421 gcagtccgag atcgacgcgc gtatcgcggc gctcacgggc gacaccgtct ccgacatcct
      481 cttcacgtcg ggcacgaccg gccggcccaa gggcgcgatg ttgacgcacg gcgccagtgt
      541 gcgcgcgtac gacgcatggg caagcgtcgt ggggctgcaa cacggcgacc gttacctcgt
      601 cgtcaacccg ttcttccacg cgttcgggct caaggccggg atccttgcga gcctcatcaa
      661 gggcgccacg atcgtgcccc acgcagtgtt cgacgtcgac caggtgatgc agcgcgtcgt
      721 cgacgagagc atctcgatgc tccccggccc gccgacggtg taccagtcga tcctcgacca
      781 cccgcgcgcg acggagttcg acatgtcgtc gctgcggctc ggtgtgacgg gcgcggcacc
      841 ggtctcggtc gagctggtgc gccgcattcg tgaggagttg aagttcgaca cgatcgtgac
      901 cggctacggc ctcacggagg cgaccggtat cgcgacgatg tgccgccacg acgatccgcc
      961 ggagaccatc tcgcgcacat cgggccgcgc gattcccggc gtcgaagtgc gcctggtcga
     1021 cgacgacaac aactcggtgc cgaccggaga gccgggcgaa gtcgtcgtcc gcggctataa
     1081 cgtgatgaag ggcttcctcg acgatcccga cgcgaccgcg gaagcgatcg acagcgaggg
     1141 ctggctgcac accggcgaca tcgcagtgat ggacgacggc gggaacgtca ccatcaccga
     1201 ccgcaagaag gacatgttca tcgtcggcgg cttcaacgcg tacccggccg agatcgagaa
     1261 catgttgagc gcgcacccgt cgatcagcca ggtcgcgatc gtcggtgtgc ccgacacgcg
     1321 catgggcgag gtcgcgtgcg cgttcgtcgt cctgcgcccc aacaccacgc tcacgccgga
     1381 cgaactcatc gcctggagcc gcgacaagat ggcgaactac aaggtccccc gccacgtcga
     1441 tatcctcgac gcgctcccgc tcaacgccag cggcaaggtc ctgaagtacg agctccgcaa
     1501 ctcccgcaaa tgatcactcg ggcgtcgctc gcccacaact attgggggtc agcgacgccc
     1561 gagcgcggtc acacggtcca gctgtagttc tcgtcgggcc agtagacgac gttgtcgttc
     1621 ttgaggtcgt ccatccgcgc cgcgtcgtag ccgagctcct cgaggatgtc cttggtgtcc
     1681 tcgccgacgc gcgggggcgg gcggtccggg ttcgtcggtg tggccgagaa gtcgaagaac
     1741 cggccgtact ggcgcatggt gccgaccacc gggtgctcgt actccgccac caggccgagg
     1801 cgttcgttgt cggcgtcgaa cagcgcgagg cggccggcca acgtgtcgac cgggacttcg
     1861 ttcggcacgc cgtggtcgtc gagcacccgt gaccacacga ctgcggtgcc accggcgaac
     1921 gcgggcgcga gcaacgcttc gagctgctgg cggtgctcgg cgcgcgcggc gggcgtcgcg
     1981 aaccgtgcgt cgtcggcgag atcgggttgg ccgaccgccg cgcacaaccc gcgccagtgc
     2041 tcgtccttca ccgccgcgat ctggatccag ccgtcgttgg tgcggtacag ccggtccgtc
     2101 gggccgaatc cggtctgatc ggcgtccaag cgcggtcggg ggacgcccac gccgtcgacg
     2161 agcatcgcgt
//
LOCUS       DASUGA010000075         2877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1787739, whole genome shotgun sequence.
ACCESSION   DASUGA010000075 DASUGA010000000
VERSION     DASUGA010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1787739"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VFX21_01350"
     CDS             <1..250
                     /locus_tag="VFX21_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX5094622.1"
                     /translation="AAAAAAVTIPTAIFVGAVWLFQVRPYAIGMLRTALFAVAAALML
                     AASFCPQPVLVAGVVFAVFMAVEVVLAGAHRTGSSVAA"
     gene            247..2049
                     /locus_tag="VFX21_01355"
     CDS             247..2049
                     /locus_tag="VFX21_01355"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019010143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX5094623.1"
                     /translation="MTVTATPPRWDLSPIFAGLDDRSFNNTLEGVYARVDRLAALYDE
                     HGIGAIEPRPTTDADIAVLDQVLGETNDMQTDMRVVSAYLYALTTTDSRDDVAAARIV
                     EMQTRTAAIGPLGKRLGSWLAALGVDAFVAKSETAAAHEYTLRKAVDNADFQMSEAEE
                     SLAAALAPSGSLAWQRLHGDVSSQLMVEVRGAGGSTERVPMAVARGLATHPDAATRRA
                     AYDGELAAWDTVAVPLAAALNGAKGEAAVLNRRRGFADDLEPNLRANNVDRATVDAMN
                     AAVIDSLPDFRRYLRAKAALLGHDRGLPWFDLLAPVGDAGEVSWPEATNLVRDAFAGF
                     SPALLGLADRAFDEQWVDAEMRDGKRGGAYCVGVAKDVSRVMMNFDGSTDSVSTLAHE
                     LGHAFHNVALAERTPLQRRLPMALAETASIFCETLLFETAAAQATDPGLRLSLLDTHL
                     TGAAQVVVDIHSRFLFETELCRRRARTALSVSELNAAMLAAQEASYGDGLDPEYRHAY
                     MWAVKPHYYTPFYNWPYTFGLLFGLGLYARYVDDPDTFRAGYDNLLSTTGMADAAELT
                     GRFGFDVRDKEFWASSLANLRGHIDEYVALAAAR"
     gene            complement(2054..>2877)
                     /locus_tag="VFX21_01360"
     CDS             complement(2054..>2877)
                     /locus_tag="VFX21_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5094624.1"
                     /translation="IRYRDFMQPQGYDDELRAVFKTGDSTWGVVGLYRDPGRKPFDED
                     DIALMRAISTPVAHALRKYVRDSTPWLAQPSAPGLVVIDSAGRATSANVDALAWLQEL
                     WPAASIPSCPVDSFDVFAKPDCDLQIPTALLALVARARAVAAGRERAPARLRLRDRRG
                     RWLVLHASPLSGADPDDDSVALIIEAAKSAEIAPIIIEAYSLTPRERDVLGAIARGGT
                     TAEIAAELFLSPHTVRDYVKAVFEKVGVSSRGELVARLFGEHYTDRLHETMVHLD"
BASE COUNT          421 a         1009 c         1008 g          439 t
ORIGIN      
        1 cgccgccgcc gccgcggcgg tcacgatccc gaccgcgatc ttcgtcggcg cggtctggct
       61 gttccaggtg cggccctacg cgatcggcat gctgcgcacc gcgctgttcg cggtcgcggc
      121 cgcgctgatg ctcgccgcga gcttctgccc gcagccggtc ttggtggccg gcgtcgtctt
      181 cgcggtgttc atggccgtgg aagtcgtgct ggccggcgcg caccgcacag gttctagtgt
      241 cgcggcatga cggtgaccgc gaccccgccg cgttgggatc tgagcccgat cttcgccggc
      301 ctcgacgacc gctcgttcaa caacacgttg gagggcgtct acgcgcgggt ggaccgcctc
      361 gccgccctct acgacgagca cggcatcggt gcgatcgaac cccggccgac aaccgacgcc
      421 gacatcgcgg tgctcgacca ggtgctcggc gagacgaacg acatgcagac cgacatgcgc
      481 gtggtcagcg cgtatctcta tgcgttgacg acgaccgaca gccgcgacga tgttgccgcc
      541 gcccgcatag tcgagatgca gacgcggacc gccgccatcg gaccgctcgg caagcgcctc
      601 ggttcgtggc tggccgcgct cggggtcgac gcgttcgtcg ccaagagcga gacggccgcc
      661 gcgcacgagt acacgctgcg caaggcggtg gacaacgccg acttccagat gagcgaagcc
      721 gaagagtcgc tcgcggccgc gctcgcgccg tcgggttcgc tggcgtggca gcggctccac
      781 ggcgacgtct cgtcgcagct catggtcgag gtgcgcggtg ccggcggttc gaccgagcgc
      841 gttccgatgg ctgtcgcgcg cggactggcg acccaccccg acgccgcgac gcgccgggct
      901 gcgtacgacg gcgagctcgc ggcgtgggac acggtcgcgg taccgctcgc ggccgcgttg
      961 aacggcgcca agggcgaggc cgcggtgttg aaccggcgcc ggggatttgc cgacgacctc
     1021 gaaccgaact tgcgcgccaa caacgtcgac cgcgcgaccg tcgacgcgat gaacgcggcc
     1081 gtcatcgatt cgctccccga cttccgtcgt tacctgcggg ccaaggccgc gttgctcggt
     1141 cacgaccgcg gcctgccctg gttcgatctg ctggcgccgg tcggggacgc gggcgaggtg
     1201 tcgtggccgg aggcgacgaa cctggtgcgc gacgcattcg ccggcttctc gcccgcgctc
     1261 ctcggcctcg ccgatcgcgc gttcgacgag caatgggtcg acgccgagat gcgcgacggc
     1321 aagcgcggcg gcgcgtactg cgtcggggtc gccaaagacg tcagccgcgt catgatgaac
     1381 ttcgacggca gcaccgactc ggtgtcgacg ctcgcgcacg agctcggcca cgcgttccac
     1441 aacgtcgcac tggccgagcg cacgccgctg cagcgacgct tgccgatggc gttggccgag
     1501 accgcgtcga tcttctgcga gacgctgctc ttcgagaccg cggctgcgca ggccaccgat
     1561 cccggcctgc ggttgtcgtt gctcgacacg cacctcaccg gcgccgcgca ggtcgtcgtc
     1621 gacattcaca gccgcttcct gttcgaaacg gagctgtgcc ggcggcgggc gcgcaccgcg
     1681 ctctcggtca gcgagctgaa tgccgcaatg ctcgcggcgc aggaggcgtc ctacggcgac
     1741 ggcctcgatc ccgagtaccg gcacgcatac atgtgggcgg tgaagccgca ctactacacg
     1801 ccgttctaca actggccgta caccttcggc ctgctgttcg ggctcggcct gtacgcgcgg
     1861 tacgtcgacg atcccgacac cttccgcgcc ggctacgaca acctgctctc cacgaccggc
     1921 atggccgacg cggccgagct cacgggccgc ttcggcttcg acgtacgcga caaggagttc
     1981 tgggcgtcga gcctggccaa cctgcgcggc cacatcgacg aatacgtcgc attggccgcc
     2041 gcgcgctgac acgtcagtcg aggtggacca tcgtctcgtg aagacgatcg gtgtagtgct
     2101 cgccgaagag ccgggcgacc agttctccgc ggctcgacac gccgaccttc tcgaacaccg
     2161 ccttgacgta gtcgcgcacg gtgtggggcg acaggaagag ctcggccgcg atctcggccg
     2221 tcgtgccgcc acgcgcgatg gcgccgagga cgtctcgttc gcgcggcgtc aacgagtacg
     2281 cctcgatgat gatgggcgcg atctcggcac tcttggccgc ctcgatgatg agtgcgaccg
     2341 aatcgtcgtc gggatcggcg ccggaaagtg gcgaggcgtg cagcaccagc cagcggccgc
     2401 ggcggtcgcg gagtcggagc cgggccggcg cgcgttcccg gccggcggcg accgcccggg
     2461 cccgcgcgac gagcgccaag agcgcggtcg ggatctggag gtcgcagtct ggcttcgcga
     2521 acacgtcgaa cgagtcgacc gggcacgacg ggatcgaagc cgccggccac agttcctgta
     2581 gccacgcgag tgcatcgaca ttcgccgatg tggcgcggcc ggcgctgtcg atgacgacga
     2641 gaccgggggc gctgggctgc gcgagccacg gtgtcgagtc gcgcacatac ttccgcaacg
     2701 cgtgcgcgac gggcgtactg atcgcgcgca tcagcgcgat gtcgtcctcg tcgaagggct
     2761 tgcgacccgg atcgcggtac aagccgacca cgccccatgt gctgtcgccg gtcttgaaca
     2821 cagcgcgcag ttcgtcgtcg tagccctgcg gctgcatgaa gtcgcggtag cggatgc
//
LOCUS       DASUGA010000076         5643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_675245, whole genome shotgun sequence.
ACCESSION   DASUGA010000076 DASUGA010000000
VERSION     DASUGA010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675245"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /gene="lepB"
                     /locus_tag="VFX21_01365"
     CDS             complement(<1..710)
                     /gene="lepB"
                     /locus_tag="VFX21_01365"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX5094625.1"
                     /translation="MDAQPAGGPVETEPTDAAPPGTIDVHAPEPPGEAPAPKRHYSPV
                     GLVVLAASVITSGILLHLNPLQWGLLIGGTVCLTLLALALAPSVLEWLVLLVTAVAIA
                     IIIKTFLVQAFYIPSGSMLPTLQENDRVLVNKLSYKMHDVHRGDIVVFEAPPGMEQRD
                     IKDLVKRVVGLPGDDVSLHDGQVYINGKALDEDYLPGGPAQTPSEACSGHTETDWKIP
                     DNAIFVMGDNRTASQDSR"
     gene            complement(803..1744)
                     /locus_tag="VFX21_01370"
     CDS             complement(803..1744)
                     /locus_tag="VFX21_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX5094626.1"
                     /translation="MSPRAVAGSEIQRILALVPWIVGHPGASKGEIARRFGITVEQLD
                     DDLGLALMIGVPPYSPGDYLDVVEDEEGHVTIQLADYFQRPLRLSPAEGLALLAAGRA
                     LLAVPGSDPEGPLATALAKLEAALDLPDLVVEVGEPSFLGGVRDAATAHERVEIDYWS
                     AGRDELTTRRIDPTVVFYAMGAWYVDAYCRRAGGERMFRVDRIRALRATGEHFEPVDE
                     DNNTNREIYNPRPDDPRVTLRLRPGAAWVVENYPTTSVTERGDGGLDVEFVVSEEPWL
                     ERLLLKLGDEAEVLEPASLQAARARVATRVLARYRGR"
     gene            complement(1741..2664)
                     /locus_tag="VFX21_01375"
     CDS             complement(1741..2664)
                     /locus_tag="VFX21_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX5094627.1"
                     /translation="MTDSPSRVERVLNLLALLLDTRVPITREELVREVSGYPPQVSAY
                     RRAFERDKEVLRGMGVPITTEQLGDGTEIGYRVKPDDYYLPDLDLTADETAALRVAVS
                     AISLGNQSGQGALMKLGEFGDAGAAPIASLPLVPALATFFEAFRRRAVATFTHRGTTR
                     RLEVWGLSSKRGHWYVVGRDLDRDAIRAFRADRIEGDVDLGPPNAFETPADFRPDDHI
                     EDRPWMMGNSGPVTVCLLVDADHAAGARAELGNEATFTERSNGDVEVELTITSRGAFV
                     NFALGFLDHAEVLSPPEVRDEMIAWLERAAR"
     gene            complement(2661..3731)
                     /locus_tag="VFX21_01380"
     CDS             complement(2661..3731)
                     /locus_tag="VFX21_01380"
                     /inference="COORDINATES: protein motif:HMM:NF024384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HEX5094628.1"
                     /translation="MPSFKSGAVVRLLEERAGLQRVEVDLGNGPERAYVLTQLTGRVD
                     AGDDVVVNTTAVELGLGTGGWHVVHWNLTRREWNEPGPGHIIKARYTSLQTDVGSTEE
                     HFPELADITSIDKMPVVAAALHSQLPAIAIAYKQRNPKGRLAYIMTDGAALPLALSDL
                     VAQLREKDLVDVTITCGHAFGGDLEAVSVYSALAAARFIANAAAAVVCMGPGIVGTAT
                     RLGFTGIEVAPILDAVDALDGVPIIALRMSDADPRERHRGVSHHTLTALAMTRSSVVV
                     PWPAMAGRDHLDDFEPATVAWGDRNPFAKHEIVHVEHLDMVKRLDAHGLRVVSMGRAA
                     SEDPVLFEAAGAAGWVAAERIP"
     gene            3774..4133
                     /locus_tag="VFX21_01385"
     CDS             3774..4133
                     /locus_tag="VFX21_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094629.1"
                     /translation="MRTPPWPSESVEWVTITLRYEPVRPDPDDDMPAALDDDFHHAVE
                     DAIAPGRRRRRWFGLVESFRGEAGTLQIFCYGPDADRMWAALEPVVGAARGVWYDGSI
                     ALSYRDGRQNETRSLSE"
     gene            complement(4125..5483)
                     /gene="pafA"
                     /locus_tag="VFX21_01390"
     CDS             complement(4125..5483)
                     /gene="pafA"
                     /locus_tag="VFX21_01390"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018219798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HEX5094630.1"
                     /translation="MERRIFGLENEYGVTCTLRGQRRLSPDEVARYLFRRVVSWGRSS
                     NVFLENGARLYLDVGSHPEYATPECDSIYDLVVHDKAGERILESLVQSAEQRLREEGI
                     RGEVFLFKNNTDSAGNSYGCHENYLVERDGDFSKFTDVLIPFLVSRQVYAGAGKVLQT
                     ARGAMFCIAQRAEHIWEGVSSATTRSRPIINTRDEPHADAERFRRLHVIVGDSNMSEY
                     ATFLKVGATSIILRMLEEDGAPWRDLTLENPIRAIREISHDISCRRKVRLANGRELSA
                     VEIQSEYLTRALKFAKRRGLPPLEQQALDMWEHVMNNLEKDPLELKTEVDWVIKYHLI
                     EAYRQRGDLPLTHPRVALLDLTYHDVTRARSLYYILEKRGDVDRIVTDDEIQEAVNTP
                     PQSTRARLRGAFIKRAKERKRDYTVDWVHLKLNDQAQRTVLCKDPFKARDERVERLIA
                     SL"
BASE COUNT          840 a         1877 c         1978 g          948 t
ORIGIN      
        1 cagcgggagt cctgcgacgc ggtgcggttg tcgcccatca cgaagatcgc gttgtcgggg
       61 atcttccagt cggtctcggt atggcccgaa cacgcctcgc tcggcgtctg cgccgggccg
      121 ccggggaggt agtcctcgtc cagcgccttg ccgttgatgt acacctggcc gtcgtgcagc
      181 gagacgtcgt cgccgggcaa tccgacgacg cgcttgacga ggtccttgat gtcgcgctgt
      241 tccatcccgg gcggcgcttc gaagacgacg atgtcgcccc gatggacgtc gtgcatcttg
      301 tacgacaact tgttgacgag cacccggtcg ttctcttgca acgtcggcag catcgagccc
      361 gacggtatgt agaacgcctg caccaggaac gtcttgatga tgatcgcgat cgcaaccgcg
      421 gtgaccagca gcacgagcca ttcgagaacc gacggtgcga gcgcgagcgc gaggagcgtg
      481 aggcacacgg tgccgccgat gaggagtccc cactgcagcg ggttgaggtg caggaggatg
      541 ccgctcgtga tgacgctcgc ggcgagcacg acgagcccga ccggcgagta gtgccgcttc
      601 ggcgcgggtg cttcgcccgg cggttcgggc gcgtgtacgt cgatggttcc ggggggtgct
      661 gcgtcggtgg gctcggtctc gaccggcccg cccgcaggct gggcgtccac gtgcgattgc
      721 tcctcgctgg gtcgggacat cctgtcatgc gcgccgtggc gtacctcttc aggtcggctg
      781 gcgccggtcc attctgagcc ggtcatcggc cgcggtagcg ggccaggacc cgcgtcgcca
      841 cgcgcgcgcg ggctgcttgc aaggatgccg gttcgaggac ctccgcctcg tctccgagct
      901 tgagcagcag acgctcgagc cagggctctt cgctcaccac gaactcgacg tcgaggccgc
      961 cgtcaccgcg ctcggtgacc gacgttgtcg gatagttctc cacgacccag gccgcacccg
     1021 ggcgcagccg cagcgtgact cgcggatcgt cgggacgcgg gttgtagatc tcacggttcg
     1081 tgttgttgtc ctcgtcgacg ggctcgaagt gctcgccagt cgcgcgtagc gcgcgaatgc
     1141 ggtcgacgcg gaacatgcgc tccccgcccg cgcggcggca gtaggcgtcg acgtaccacg
     1201 cccccatcgc gtagaagacc accgtcgggt cgatgcgccg cgtcgtgagc tcgtcacggc
     1261 cggccgacca gtagtcgatc tcgacccgct cgtgcgcggt ggccgcatct cgcaccccgc
     1321 cgaggaacga cggctcgccc acctcgacga cgaggtcggg caagtcgaga gcggcttcca
     1381 gcttggccag cgcagtggcg agcgggccct cgggatcgga gccggggacg gcgagcaacg
     1441 cgcggcccgc ggcgagcagc gcgaggcctt cggccggcga cagccgcagc ggccgctgga
     1501 agtagtcggc gagctggatg gtgacgtgcc cctcctcgtc ctcgacgacg tcgaggtagt
     1561 cgcccggcga gtacggcggc acgccgatca tcaatgccag cccgagatcg tcgtcgagct
     1621 gttcgacggt gatgccgaac cggcgcgcga tctcgccttt cgacgccccg ggatggccga
     1681 cgatccacgg caccaacgcg aggatgcgct ggatctccga tccggcgacg gcgcgggggc
     1741 tcaccgggcc gctcgttcca gccaggcgat catctcgtcg cgaacttccg ggggcgacag
     1801 cacttcggcg tggtcgagga agccgagcgc gaagttgacg aatgcgcccc ggctggtgat
     1861 cgtgagctcg acctccacat cgccgttcga acgctcggtg aacgtcgctt cgttcccgag
     1921 ctcggcgcgc gcgccggccg cgtggtcggc gtcgacgagg aggcagacgg tgacggggcc
     1981 actgttgccc atcatccacg gccggtcctc gatgtggtcg tcggggcgga agtcggcggg
     2041 cgtctcgaac gcgttcggtg gtccgaggtc gacgtcgccc tcgatgcggt cggcacggaa
     2101 cgcgcggatc gcgtcccggt cgaggtcgcg gccgacgacg taccagtgac cccgcttcga
     2161 cgacaggccc cagacttcca accttcgtgt cgtaccgcgg tgggtgaacg tcgcaaccgc
     2221 gcgccgacgg aacgcttcga agaacgtcgc gagcgcggga acgagcggca gcgatgcgat
     2281 cggtgccgcg cccgcgtcgc cgaactcgcc gagcttcatc aacgcgccct ggccactctg
     2341 gttgccgagc gagatcgcgc tcaccgcgac ccgcaacgcc gcggtctcgt cggcggtgag
     2401 gtcgaggtcc ggcaggtagt agtcgtcggg cttgacgcgg taaccgatct cggtgccgtc
     2461 gccgagctgc tcggtggtga tcggcacacc catcccgcgc agcacctctt tgtcgcgctc
     2521 gaacgcacgc cggtacgccg atacctgcgg cgggtagccg ctgacctccc gtaccagctc
     2581 ctcgcgcgtg atcggcacgc gcgtgtcgag caagagcgcg agcaggttga ggactcgctc
     2641 gacgcggctc ggtgagtcgg tcacggaatg cgctccgcgg caacccatcc cgcggcgccg
     2701 gccgcttcga acaagaccgg gtcctcgctc gccgcccgac ccatggagac gacacggagg
     2761 ccgtgcgcgt cgagccgctt caccatgtcg agatgctcga cgtggacgat ttcgtgtttc
     2821 gcgaacgggt tgcgatcgcc ccacgcaacg gtcgcgggct cgaagtcgtc gaggtgatcg
     2881 cggccggcca ttgccggcca cggcacgaca accgacgacc tcgtcatcgc gagcgcggtc
     2941 agcgtgtgat gagacacgcc gcgatggcgt tctctgggat ccgcgtcgga catgcgcagc
     3001 gcaatgatcg gcacgccgtc gagcgcgtcg acggcgtcga ggatcggcgc cacttcgatg
     3061 ccggtgaagc cgaggcgcgt tgccgtgccc acgattcccg gacccatgca gacgaccgcg
     3121 gccgccgcgt tggcgatgaa gcgagcagcc gcgagcgcgc tgtagaccga caccgcttcg
     3181 aggtcgccgc cgaacgcgtg gccacacgtg atggtcacgt cgacgaggtc cttctctctg
     3241 agctgggcca cgagatcgga gagggcgagc gggagcgccg caccgtcggt catgatgtac
     3301 gcgagccgcc ccttcggatt gcgttgtttg tacgcgatcg cgatggccgg cagctgactg
     3361 tgcaaggccg cggcgacgac aggcatcttg tcgatcgacg tgatgtcggc gagctcgggg
     3421 aagtgctcct ccgtgctgcc gacgtcggtc tgcaggctcg tgtagcgcgc cttgatgatg
     3481 tggccgggtc cgggctcgtt ccactcccgc cgcgtcaggt tccagtgcac gacgtgccag
     3541 ccgccggtac cgaggccgag ctcgaccgcg gtcgtgttga cgacgacgtc gtcgccggcg
     3601 tcgacgcggc ccgtgagctg cgtcagcaca taggcgcgct cgggtccgtt ccctagatcg
     3661 acctcgacgc gctgcaaccc ggctcgctct tcgagcaggc ggaccacggc gccggacttg
     3721 aacgacggca tcacggggca ggttacgggg cgccggcgaa taccttgtgc cccgtgcgga
     3781 caccgccatg gccgagcgaa tccgtcgaat gggtgacgat cacgctgcgc tacgaacccg
     3841 ttcgaccgga tccggacgac gacatgccgg cggcgctcga cgacgacttc caccacgcgg
     3901 tcgaggatgc gatcgcaccg ggccggcgcc ggaggaggtg gttcggtttg gtcgagagct
     3961 tccgtggtga agcggggaca ctgcagatct tctgctacgg accggacgcc gaccgcatgt
     4021 gggccgctct cgaaccggtc gtcggcgctg cgcgcggcgt ttggtacgac ggatcgatag
     4081 cgctcagcta ccgcgacggc cggcagaacg agacgcgttc gctgtcagag tgacgcgatc
     4141 agccgttcga cgcgttcgtc gcgcgccttg aacgggtcct tgcacaacac ggtgcgctgc
     4201 gcctggtcgt tgagcttcaa gtgcacccag tcgaccgtgt agtcgcgctt gcgctccttg
     4261 gcccgcttga tgaacgcgcc ccgcaagcgg gcgcgggtgc tctgcggcgg cgtgttgacc
     4321 gcttcttgga tctcgtcgtc ggtgacgatg cggtcgacgt cgccgcgctt ttcgagtatg
     4381 tagtagagcg accgcgcccg ggtgacgtcg tggtacgtga ggtcgagcag cgcaacgcgt
     4441 gggtgcgtga gcggcagatc gccgcgctgc cggtacgcct cgatgaggtg gtacttgatg
     4501 acccagtcga cttcggtctt cagttcgagc gggtccttct ccaaattgtt catcacgtgt
     4561 tcccacatgt cgagcgcttg ctgctcgagc ggtggcaacc cgcgccgctt ggcgaacttc
     4621 agcgcgcgtg tgaggtactc cgactggatc tcgaccgcgg acagctcgcg gccgttcgcg
     4681 aggcggacct tgcgccggca cgagatgtcg tggctgatct cgcgaatggc gcggatcgga
     4741 ttctcgagcg tgaggtcacg ccacggtgca ccgtcttctt cgagcatccg caagatgatc
     4801 gacgtcgcgc cgaccttcag gaacgtcgcg tactcgctca tgttcgagtc gccgacgatg
     4861 acgtgcaggc gccggaaccg ctcggcatcg gcgtgcggct cgtcgcgcgt gttgatgatc
     4921 ggccgggacc gggtggtcgc gctcgacacg ccttcccaga tgtgctcggc gcgctgcgcg
     4981 atgcagaaca tggcgccgcg cgccgtctgc agcaccttcc ccgcgcccgc gtagacctgg
     5041 cggctgacga ggaacgggat cagcacgtcg gtgaacttgg agaagtcgcc gtcgcgctcg
     5101 accagatagt tctcgtggca gccgtaggag ttgccggcac tgtcggtatt gttcttgaac
     5161 aggaagacct cgccgcgaat gccctcttcg cgcaggcgct gttcggcgct ctggacgagg
     5221 ctctcgagga tccgctcccc cgccttgtcg tggacgacga ggtcgtagat cgagtcgcat
     5281 tcgggcgtcg cgtactcggg gtggctgccg acgtcgaggt agaggcgggc accgttctcg
     5341 aggaacacgt tcgacgaccg gccccacgag acgacgcggc ggaacaaata ccgcgccact
     5401 tcgtcggggc tcaggcgccg ctgcccgcgc agcgtgcacg tgacgccgta ctcgttctcg
     5461 aggccgaaga tgcgccgctc cactggcggg gagtgtacgg ggcgggcgga tgaccccagt
     5521 gccgtcctcg tacactccgg gcgtggcgct gaccagggtg acctcggtct acggctcctt
     5581 ccgagcccgt gtcgtcgccg cacgactcga atacgagggc ttcgaagtcg tgctccgcgg
     5641 tcc
//
LOCUS       DASUGA010000077         3450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_259939, whole genome shotgun sequence.
ACCESSION   DASUGA010000077 DASUGA010000000
VERSION     DASUGA010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259939"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFX21_01395"
     CDS             complement(<1..342)
                     /locus_tag="VFX21_01395"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX5094631.1"
                     /translation="MDFGRNAGVLVAATVIAVDQWTKLLASSDASLHNANYAFGIVRG
                     ATPVLVAGTVSVLLLFVGVVGRIATRLGIPVVLPAFVAGGMVSNAIDRVVFGSVRDHI
                     VTPWAIVNVADV"
     gene            375..1436
                     /locus_tag="VFX21_01400"
     CDS             375..1436
                     /locus_tag="VFX21_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX5094632.1"
                     /translation="MDPPALSAALRAVIDAKGTDLVLSAGEPPLVRVDGALAAVRDLP
                     PIDDDAIGAMLEALVSSELRAQLEHDRDIDFAFTRDDVRFRGNAFFQRGKLGIALRRL
                     HDTIPTCEELGMPPVVGDLVTRKQGLILFTGPTGSGKSTSMAALVGRVNRMRPCHIIT
                     IEDPIEYVHRNEQAVVHQREVGIDALSFERALRAALREDPDIVLVGEMRDPESIAIAL
                     TLAETGHLVLSSLHTNDAPQALDRIVDVFPAERQAQVRLQLASTLVAVVAQRLLPAAT
                     GGLVAAFEVMVATSAVSNLIREGKTRQLRNAMQMGMAAGNRTMEMALNELLASGAITQ
                     ETATAAAFVASEVDPTRPS"
     gene            1463..1936
                     /locus_tag="VFX21_01405"
     CDS             1463..1936
                     /locus_tag="VFX21_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEX5094633.1"
                     /translation="MARWLLKSEPDVFSYDDLVRAKREGWDGIRNYQARNFMREMRKG
                     DEAIFYHSQSKPPGVAGICKIVKTAEPDPTQFDPTSKYHDPASKPEDPRWDWVTVAPV
                     RALPFVALDELRALPELAGCRLLARGNRLSVFPLTDVEFDTIVRYATSPRMPPQA"
     gene            1953..3383
                     /locus_tag="VFX21_01410"
     CDS             1953..3383
                     /locus_tag="VFX21_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004531381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5094634.1"
                     /translation="MTTTDKIYIGGAWVPSTGTGTIDVFDSTDGSVIAQIPDGTADDV
                     DRAVTAACAAFGDWAARDPEERGKFMSRIGEGLSGRMDEIATVVTREAGMPKWLSMIV
                     QAGLPINSFGTAGALAGTYKYEEENGNSLIVREPVGVVGCITPWNYPLHQIAAKVAYA
                     MAAGCTVVLKPSEVAPLDAFILAEVIHDVGLPAGVFNMVAGTGATVGEAIARHPRVDM
                     VSFTGSTRAGRRVAELASQSLKRVALELGGKSANILLDDLDDAAFDKAVRDGIQKCYL
                     NSGQTCSALTRMLVPSSRLADAERIAGEEVEANWQPGDPFAEGARLGPLASQAQVERV
                     NGYIQKGIDEGAKLVTGGTGKPEGLDQGYFVRPTVFSGVSNDMTIAQEEIFGPVLSII
                     PYADEDDAVRIANDSNYGLAGGVWGGDADRAKKVARRIRTGQIEVNGGAFNPNAPFGG
                     FKQSGYGREYGPHGFEEFLEIKSMQL"
BASE COUNT          588 a         1193 c         1165 g          504 t
ORIGIN      
        1 gacgtcggcc acgttgacaa tcgcccacgg cgtcacgatg tggtcgcgaa cgctgccgaa
       61 gacgacccgg tcgatcgcgt tcgacaccat cccgcccgcg acgaacgccg ggagcacgac
      121 ggggataccg agccgcgtcg caatgcggcc gacgacgccg acgaacagaa ggaggacgct
      181 cacggttccc gcgaccaaca ccggcgtcgc gccccgcacg atcccgaatg cgtagtttgc
      241 gttgtgcagc gacgcgtcgg agctcgccaa cagcttcgtc cactggtcga ctgcgatcac
      301 cgtcgccgcg accagtacgc ccgcgttccg cccgaagtcc acgcttcatc atcggccgtt
      361 acgcttgcgc cgtgatggat cccccggcgt tgtccgccgc gctgcgagcc gtcatcgacg
      421 ccaagggaac cgacctggtg ctgtccgcgg gtgaacctcc gctcgtgcgt gtggacggcg
      481 cgctcgccgc ggtgcgcgat ctcccgccga tcgacgacga cgcgatcggg gcgatgctcg
      541 aagcgctcgt gagcagcgag ctgcgcgcgc aactcgagca cgaccgcgac atcgacttcg
      601 cattcacccg cgacgacgtg cgctttcgtg gcaacgcgtt cttccagcga ggcaagctcg
      661 gcatcgcgct ccgtcgcttg cacgacacga tcccgacgtg cgaagagctg ggcatgccgc
      721 ccgtcgtcgg tgacctcgtg acgaggaagc aggggttgat cctcttcacg ggcccgaccg
      781 ggtccggcaa gtcgacgagc atggccgcgc tcgtcgggcg tgtgaaccgg atgcgcccgt
      841 gccacatcat cacgatcgaa gatccgatcg agtacgtgca tcgcaacgag caagccgtcg
      901 tccaccaacg cgaggtcggc atcgacgcac tgtcgttcga gcgggcgttg cgcgccgcgc
      961 tacgcgaaga ccccgacatc gtgctcgtcg gcgagatgcg cgatcccgag agcatcgcga
     1021 tcgcgctgac cctggccgag acggggcacc tcgtgctgtc gtcgctgcac acgaacgacg
     1081 cgccgcaggc gctcgaccgc atcgtcgacg tctttccggc cgagcggcag gcgcaagtgc
     1141 gactgcagct cgcgagcacg ctcgtcgccg tcgtcgcgca gcggctcctc cccgcggcca
     1201 caggcggcct ggtcgccgcg ttcgaggtca tggtcgcgac gtccgcggtc agcaacctga
     1261 tccgtgaggg caagacgcgc cagctccgca acgcgatgca gatgggcatg gcggccggca
     1321 accgcacgat ggagatggcc ctcaacgagc tgctggcgag tggcgcgatc acgcaggaga
     1381 ccgcgaccgc ggccgcgttc gtggcgagcg aagtcgaccc gacgcgcccg tcctaggtgc
     1441 tccacccacg tagcctcggg ccgtggcccg gtggttgctg aagtccgaac ccgacgtctt
     1501 ctcttacgac gacctcgtgc gcgcgaagcg cgaagggtgg gacggcatcc gcaactacca
     1561 agcccgcaac ttcatgcgcg agatgcgcaa gggcgacgag gcgatcttct atcactcaca
     1621 gtcgaagccg cccggcgtcg ccgggatctg caagatcgtc aagaccgcgg agcccgatcc
     1681 gacgcagttc gaccccacga gcaagtacca cgaccccgcg agcaagcccg aagacccgcg
     1741 ctgggactgg gtgaccgttg cgcccgtgcg cgcgttgccg ttcgtcgcgc tcgacgagct
     1801 ccgtgcgctg cccgagctcg ccgggtgccg cttgctcgca cgcgggaacc ggctgtcggt
     1861 gttcccgttg acggacgtcg agttcgacac catcgtccgg tacgcgacca gcccgaggat
     1921 gccgccgcag gcgtagcggt atcgtcgaag gcatgacgac gacggacaag atctacatcg
     1981 gcggcgcctg ggtgccgtcg accggtaccg gcaccatcga cgtgttcgac tcgaccgacg
     2041 gctcggtcat cgcccagatc cccgatggca ccgccgacga cgtcgaccgt gcggtcacgg
     2101 ccgcgtgcgc cgcgttcggc gactgggccg cgcgcgatcc cgaagagcgc ggcaagttca
     2161 tgtcgcgcat cggcgaaggc ctcagtggtc gcatggacga gatcgcgacg gtcgtcaccc
     2221 gcgaggcggg catgccgaag tggctgagca tgatcgtgca ggccgggctg cccatcaact
     2281 cgttcggtac cgcgggcgcg ctcgcgggca cctacaagta cgaagaggag aacggcaact
     2341 cgctcatcgt gcgtgaaccc gtcggcgtcg tcgggtgcat cacgccgtgg aactacccgc
     2401 tgcaccagat cgcggcgaag gtcgcgtacg cgatggccgc cggttgcacc gtcgtgctca
     2461 agcccagtga agtcgcaccg ctcgacgcgt tcatcctcgc cgaagtgatc cacgacgtcg
     2521 gcctgcccgc cggtgtgttc aatatggtcg ccggcacggg cgcaaccgtc ggcgaggcca
     2581 tcgcgaggca tccgcgggtc gacatggtgt cgttcaccgg ttcgacgcgc gccggacggc
     2641 gcgtcgccga gctcgcgtcg cagtcgttga agcgcgtcgc gctggagctg ggcgggaagt
     2701 cggcgaacat cctgctcgac gacctcgacg acgccgcgtt cgacaaggcc gtacgcgacg
     2761 gcatccagaa gtgctacctg aactccggcc agacgtgctc cgcgctgacg cgcatgctgg
     2821 tgccgagcag ccggctcgcg gacgccgagc gcatcgcggg cgaagaggtc gaggcgaact
     2881 ggcagcccgg cgatccgttc gcggaaggag cgcgtctcgg tccgctcgcg tcgcaggcgc
     2941 aggtcgaacg ggtcaacggc tacatccaga agggcatcga cgaaggcgcg aagctcgtca
     3001 ccggcggcac cggcaagccg gagggcctcg accagggcta cttcgtacgg ccgaccgtgt
     3061 tctccggtgt ctccaacgac atgacgatcg cgcaggaaga gatcttcggc cccgtgctct
     3121 cgatcatccc ctacgcggac gaggacgacg ccgtgcgcat cgcgaacgac agcaactacg
     3181 gcttggcggg tggtgtgtgg ggcggcgacg cggatcgtgc gaagaaggtc gcgcgtcgca
     3241 tccgcaccgg gcagatcgaa gtcaacggcg gcgcgttcaa ccccaacgca ccgttcggcg
     3301 gcttcaagca atcgggctac ggccgcgaat acgggcccca cggcttcgag gagttcctcg
     3361 agatcaagtc gatgcagctc tgaatcgcgg tgcggttcgc tacaagatga cggtgaacgc
     3421 accggccaag gtggcggcgc cgccgtccgg
//
LOCUS       DASUGA010000078         7519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_118421, whole genome shotgun sequence.
ACCESSION   DASUGA010000078 DASUGA010000000
VERSION     DASUGA010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7519
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118421"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /gene="trpB"
                     /locus_tag="VFX21_01415"
     CDS             <1..1136
                     /gene="trpB"
                     /locus_tag="VFX21_01415"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX5094635.1"
                     /translation="ETLVPALIELEDEFRRAWASDEFRAEFAELLRSYAGRPTPVTEC
                     HNLSRRLGLRVLLKREDLTHTGSHKINNVLGQALLTRRMGKQRVIAETGAGQHGVATA
                     TAAALFGLECTVFMGAVDVERQALNVWRMRLLGAEVVPVNSGSATLKDAVNEAMREWV
                     ATVTDTHYCLGSAMGPHPFPWMVREFQRILGDEARAQCRAILGGADPDVVVACVGGGS
                     NAIGTFTAFVETDARLVGVEAGGLGLESGRHGAAVARGVPGVVHGMRSLFLQDEHGQV
                     LEATSISAGLDYPGVGPEHAMLAAQGRAQYFAATDEWALEGFQLLAATEGIVPALEPS
                     HAIGWLAHAVGTEVPEGATVLVTLSGRGDKDAAQVAERLGELE"
     gene            1133..1903
                     /gene="trpA"
                     /locus_tag="VFX21_01420"
     CDS             1133..1903
                     /gene="trpA"
                     /locus_tag="VFX21_01420"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX5094636.1"
                     /translation="MNLEKVLREQRAAGRKLLVPYLTGGLGSQWCDAVRAVVDGGADA
                     VEIGIPFSDPIMDGMTIQEASQRALDLGATPASVISELANVDVGVPLVVMTYYNLVAR
                     MGHERFAQSLRASGVDGAILPDLPLDELDGWGDVADAHDIESVLLAAPTSTDERLAAI
                     CARSRGFVYGVSLLGVTGERDQLAGHAEQMGRRLKACTDKPVLLGVGISTPEQAARAA
                     ASSDGVIVGSALVRRLLDGGGPEEARSFISTMRAALDA"
     gene            2068..2859
                     /locus_tag="VFX21_01425"
     CDS             2068..2859
                     /locus_tag="VFX21_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HEX5094637.1"
                     /translation="MEKGTMVGLGVTLFGVFVSAIMHGIQPVFLFMEFGSLIIVFGGC
                     FGATITSFPFAATQNLGKYIQKAMKGDEPHSASETIGQIVLLTNRARAEGLLALEEEA
                     KKIEDPFFRKGIELAVDGTDPEALKKTLFAEITAMKERHKVGQNWMTQAGVFAPTFGI
                     IGAVFGLMATMAKLDQPSEVGHGIAGAFVATFWGVFLANGIFLPIANKLKQFSQEEVA
                     HKMLIVEGIMAIQAGISPRVIEEMLKSHVPPAEREAVGQGQAEAA"
     gene            2873..3850
                     /locus_tag="VFX21_01430"
     CDS             2873..3850
                     /locus_tag="VFX21_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEX5094638.1"
                     /translation="MSHGGDGGLPEEHEEHVNHEAWVIPYADLLTLLMAMFIALFAMS
                     TVDMDKFKSLSLGFNEALGGGELSTGVFDPSKGGDGPLDGEGNGVSPQDGGEIGPDKQ
                     PKSDSFLASLLQQKLEEQVGQAQEREALEGVQHAIEAAAEEGGFNGSLETRQEERGLV
                     VTVVTDQVVFPSGSAQIEGPGLDILHVVGEALKSIDNEVIVEGHTDPEPIQNSVYRNN
                     RDLSNARASSVVTYFESLGIDGARMSSVGWADKHPLPDADNSTVEGRARNRRVEIVVV
                     SKQVQKVLEDNHLGNTPVDPSTDGVDTPAEERVDGGIDSIEPNLGTNGH"
     gene            3891..5087
                     /gene="icd"
                     /locus_tag="VFX21_01435"
     CDS             3891..5087
                     /gene="icd"
                     /locus_tag="VFX21_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX5094639.1"
                     /translation="MAEKITMQSDGTLNVPNEPIIPFVEGDGTGIDIWPAAQLVMDAA
                     AAKHGKRIEWKEVLAGEKAFNATGSWLPDETVATFREYLIGIKGPLTTPVGGGIRSLN
                     VALRQILDLYVCLRPVRWFQGVPSPVKHPELVDMVIFRENTEDIYAGLEVEEGTPEAK
                     KLIETLKQQFGWDIREDSGVGIKPVSITGSKRLIRAAIQYAVDKKRPSVTLVHKGNIQ
                     KFTEGAFRNWGYELVREEFSDVAVGWDDCGGKPGDKVLVKDNIADITLQQILTRPDDF
                     DVIATTNLNGDYLSDALAAQVGGIGIAPGGNINYVTGHGIFEATHGTAPKYAGQDKVN
                     PSSVLLSGVMMFEHLGWHEAASDIERALEATIAEKIVTYDFARLMEGATEVKTSQFAQ
                     AIVDRL"
     gene            5189..6115
                     /locus_tag="VFX21_01440"
     CDS             5189..6115
                     /locus_tag="VFX21_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094640.1"
                     /translation="MKHIDTALHKCAARQYGVITRWQLLAHGTKDQIKVRLKSGQLER
                     RYDCVYISPSVPPSWEQDELAACVAGGRPNGVASLRAAARLWGLPGGEEIVEVTTARH
                     NRLQYEGVIAHESRFLEERDRMLLNSIPVTRPERTLLDLAGLVELHRLPERTLELALF
                     EAVRRDLVDIPSVWREYERLGHYKRLGGEVILPILERFVAPIRKPETTPETELLFMLR
                     EAGFPDPVAQYWLVLPNGERIRLDYAWPWLRCGAEFDPYKFHGDRERYEKNAARTRLM
                     QAMGWSRVTVTDDDLDAGIPQTIMALRTLLAA"
     gene            complement(6158..6994)
                     /gene="rfbD"
                     /locus_tag="VFX21_01445"
     CDS             complement(6158..6994)
                     /gene="rfbD"
                     /locus_tag="VFX21_01445"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX5094641.1"
                     /translation="MSRVLVTGAAGQVGREVVELLGASAIPFDHSSLDVANREQVLAV
                     VSRERPDAIVHCAAFTAVDKCESERDAAFTVNQMGNRYVQQAAFDIGAYLVTISTDYV
                     FDGLKEGPYVETDATNPQSAYGQSKLAGEIEVDVSTSAVVRASWIVGRYGANMVKTIL
                     RLAGEHETLTFVDDQVGHPTIAADLASMLVRFVDQRLTGIWHVTNQGAVSWYEFAQEV
                     LRATNQDPERVKPITTAELDPPRPAPRPANSVLANAKLVQAGIPLLPDFRPSLLELVG
                     RL"
     gene            complement(6991..>7519)
                     /locus_tag="VFX21_01450"
     CDS             complement(6991..>7519)
                     /locus_tag="VFX21_01450"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX5094642.1"
                     /translation="SPYSASKAGSDLIALSYHSTYGLPVLVTRSSNQYGPYQFPEKVI
                     PLFVTNLLEGTRVPLYGDGLNVRDWCFVEDNCAAIDRVLRTGAVGEIYNIGGGNEISN
                     RELTDRILAHLDADESMIEYVADRLGHDRRYSIATDKVEALGWRPQRDLAEALTETVH
                     WYRDNQWWWEPLKQS"
BASE COUNT         1302 a         2462 c         2590 g         1165 t
ORIGIN      
        1 ccgagacgct cgtcccggcg ctgatcgagc tggaggacga gttccggcgg gcctgggcgt
       61 ccgacgaatt ccgcgccgag ttcgccgagc tgctccgcag ctacgcggga cgccccacgc
      121 cggtcaccga gtgccacaac ctgtcgcggc gcctcggcct gcgggtgctg ctcaagcggg
      181 aggacctcac ccacaccggg tcgcacaaga tcaacaacgt gctcggccag gcgctgctca
      241 cgcgccgcat gggcaagcag cgcgtgatcg cggagaccgg tgccggccag cacggtgtcg
      301 cgaccgcgac cgccgccgcg ctgttcgggc tcgagtgcac cgtgttcatg ggcgcggtcg
      361 acgtcgaacg gcaggcgctg aacgtgtggc gcatgcgcct gctcggcgcc gaagtcgtgc
      421 cggtgaactc cgggagcgcg acgctcaagg acgccgtcaa cgaagcgatg cgcgagtggg
      481 tcgcgaccgt caccgacacg cactactgcc tcggctcggc gatgggtccg catccgttcc
      541 cgtggatggt gcgcgagttc cagcgcatcc tcggcgacga ggcgcgcgcg caatgccgcg
      601 cgatcctggg cggcgcggac cccgacgtcg tcgtcgcgtg cgtcggcggc ggctccaacg
      661 cgatcggcac gttcacggcg ttcgtcgaga cggacgcgcg actggtcggg gtcgaagccg
      721 gcggactcgg tctcgaatcg ggccggcacg gcgcggcggt ggcgcgtggc gtccccggcg
      781 tcgtgcacgg gatgcggtcg ttgttcttgc aggacgagca cggacaggtg ctcgaagcga
      841 cgtcgatcag cgccggtctc gactatccgg gtgtcggccc cgagcacgcg atgctcgccg
      901 cgcagggtcg agcgcagtac ttcgcggcga ccgacgagtg ggcgctcgaa ggattccagc
      961 tgctcgcggc gaccgagggc atcgtgcccg cgctggagcc gtcgcacgcg atcggttggc
     1021 tcgcgcacgc ggtgggcacc gaggtgccgg aaggtgcgac cgtcctggtg accttgtccg
     1081 gccggggtga caaagacgcc gcgcaagtcg ccgaacgcct gggcgagctc gagtgaatct
     1141 cgagaaggtg ctgcgcgagc agcgggcggc cgggcggaag ctcctcgtcc cgtacctgac
     1201 cggtgggctc ggcagtcagt ggtgcgatgc cgtgcgcgcg gtcgtcgacg gcggcgcgga
     1261 cgcggtcgag atcggcatcc cgttctcgga tccgatcatg gacggcatga cgatccagga
     1321 ggcgtcgcag cgcgcactcg atctgggcgc gacgccggcg agcgtcatct ccgagctggc
     1381 gaacgtcgac gtcggcgtcc cgctcgtcgt gatgacgtac tacaacctcg tcgcgcgcat
     1441 ggggcacgag cggttcgccc agtcgctgcg cgcatcgggg gtcgacggcg cgatcctccc
     1501 ggacctgcca ctggacgagc tcgacgggtg gggcgacgtc gccgacgccc acgacatcga
     1561 gagtgtcctg ctggccgcgc cgacgtcgac cgacgagcgg ctggccgcga tctgcgcccg
     1621 ctcgcggggg ttcgtgtacg gcgtctcgct gctcggcgtc accggcgagc gagaccagct
     1681 cgccggccat gccgagcaga tgggccgccg gctcaaggcc tgcaccgaca agcccgtcct
     1741 gttgggggtc gggatctcca cgcccgagca ggcggcccgg gcggccgcca gctccgacgg
     1801 cgtcatcgtc ggcagcgccc tggtgcggcg gctcctcgac ggcggcgggc ccgaggaagc
     1861 ccgctccttc atctccacca tgcgagcggc cctcgacgca tagtcgcacg gcgccgtgcc
     1921 ggagcctgcg gaggcactgg ccggagcgga gggcgggcca acaatccggg ctgcctcacg
     1981 cgagcacgac cggagcgaca aagagagatc gtctacagcg ggtctgcgga gccgtcgata
     2041 cctcctcggg catcccaggg aggggacatg gaaaagggca cgatggtcgg gctcggcgtg
     2101 acgctgttcg gcgtcttcgt gagcgcgatc atgcacggca tccagccggt cttcctcttc
     2161 atggagttcg gctcgctgat catcgtgttc ggtggttgct tcggcgcgac catcacctcg
     2221 tttccgttcg ccgcgacgca gaacctgggc aagtacatcc agaaggcgat gaagggcgac
     2281 gagccgcact ccgcctcgga gacgatcggc cagatcgtcc tgctcacgaa ccgggcccgc
     2341 gccgagggtc tgctcgcgct cgaagaagag gccaagaaga tcgaagaccc gttcttccgc
     2401 aagggcatcg agctcgcggt cgacggcacc gaccccgaag cgttgaagaa gacgctcttc
     2461 gcggagatca ccgcaatgaa ggagcggcac aaggtcggcc agaactggat gacgcaagcc
     2521 ggcgtgttcg caccgacctt cggcatcatc ggcgccgtgt tcggcctcat ggccaccatg
     2581 gcgaagctcg accagccgag cgaggtcggt cacggcatcg ccggcgcctt cgttgcgacg
     2641 ttctggggcg tgttcctcgc caacgggatc ttcctcccga tcgcgaacaa gctgaagcag
     2701 ttctcgcaag aagaagtcgc gcacaagatg ctgatcgtcg aagggatcat ggcgatccaa
     2761 gccggcatca gcccgcgcgt catcgaggag atgctgaagt cgcacgtgcc gccggccgag
     2821 cgtgaggccg tcggtcaagg tcaagccgaa gcggcatagc ggttgacgcg cgatgtcaca
     2881 cggaggtgac ggcgggctcc ccgaggagca cgaagagcat gtgaaccacg aggcgtgggt
     2941 catcccgtac gccgacttgt tgacgctgct catggccatg ttcatcgcgc tgttcgcgat
     3001 gtcgaccgtc gacatggaca agttcaagtc gctgtcgctc ggcttcaacg aagcgctcgg
     3061 cggcggcgag ctgtcgacgg gtgtgttcga cccgagcaag ggcggcgacg gtccgctcga
     3121 cggcgagggc aacggcgtgt cgccgcaaga cggcggcgag atcggccccg acaagcagcc
     3181 gaagagcgac tcgttcctcg cgtcgctgtt gcaacagaag ctcgaggaac aggtcgggca
     3241 ggcgcaggaa cgcgaagcgc tcgaaggcgt gcagcacgcg atcgaagcgg ccgcggagga
     3301 gggcgggttc aacggatcgc tcgagacccg ccaagaagag cgcggcctcg tcgtgacggt
     3361 cgtgaccgac caggtggtct tccccagcgg gtcggcccag atcgaggggc ccggcctcga
     3421 catcctgcac gtcgtcggtg aagcgctgaa gagcatcgac aacgaggtga tcgtcgaagg
     3481 tcacaccgac cccgagccga tacagaactc cgtctaccgc aacaaccgcg acctctcgaa
     3541 cgcgcgtgcc agctcggtgg tgacgtactt cgagtcgctg ggcatcgacg gcgcgcgcat
     3601 gagctcggtc ggctgggccg acaagcaccc gctgccggac gccgacaact cgaccgtcga
     3661 gggccgcgcc cgcaaccggc gcgtcgagat cgtcgtcgtg tcgaagcaag tgcagaaggt
     3721 gctcgaggac aatcacctcg gcaacacgcc ggtcgatccg tcgaccgatg gcgtcgacac
     3781 gccggcggag gagcgggtcg acggcggcat cgactcgatc gagccgaacc tcggcaccaa
     3841 cggccactga gtcggcggaa cgccaggggt cgtccggtag cttctcggcc atggccgaga
     3901 agatcacgat gcaatccgac gggacgctga acgtccccaa tgagccgatc atcccgttcg
     3961 tcgagggaga cggcaccggc atcgacatct ggcccgcagc gcagctcgtc atggacgccg
     4021 ccgcagccaa gcacggcaag cgcatcgagt ggaaggaagt gctcgcgggc gagaaggcgt
     4081 tcaacgccac cgggtcgtgg ctgcccgacg agaccgtcgc gacgttccgc gagtacctga
     4141 tcgggatcaa gggcccgctg accacaccgg tcggcggcgg catccgttcg ttgaacgtcg
     4201 cgctgcgcca gatcctcgac ctctacgtgt gcctgcggcc ggtgcggtgg ttccagggtg
     4261 tgccgtcgcc ggtgaagcac cccgagctcg tcgacatggt catcttccgc gagaacaccg
     4321 aagacatcta cgccggtctc gaagtcgaag agggcacgcc cgaggccaag aagctcatcg
     4381 agacgttgaa gcaacagttc ggctgggaca tccgtgaaga ctcgggggtc gggatcaagc
     4441 cggtgtcgat caccggttcg aagcgcctga ttcgcgccgc catccagtac gcagtcgaca
     4501 agaagcgtcc gtcggtgacg ctcgtgcaca agggcaacat ccagaagttc accgaaggcg
     4561 cgttccgcaa ctggggctac gagctcgtgc gcgaggagtt ctccgacgtc gcggtgggct
     4621 gggacgactg cggcggcaag cccggcgaca aggtcctcgt caaggacaac atcgccgaca
     4681 tcaccctgca gcagatcttg acgcgccctg acgacttcga cgtgatcgca acgaccaacc
     4741 tgaacggcga ctacctctcc gacgcgctgg ccgcgcaggt cggcggcatc ggcatcgcac
     4801 cgggcggcaa catcaactac gtcaccggcc acggcatctt cgaagcgacc cacggcaccg
     4861 cgccgaagta tgcgggccag gacaaggtga acccgtcgtc ggtgctgctg tcgggcgtca
     4921 tgatgttcga gcaccttggg tggcacgagg ccgcgtcgga catcgaacgc gcgctggaag
     4981 cgacgatcgc cgagaagatc gtcacctacg acttcgcccg cctcatggag ggcgccacag
     5041 aagtcaagac cagccagttc gcccaagcca tcgtcgaccg cctctagccg gtcccttgta
     5101 gttggtcggg cgtcttacaa gggcgaattc cgccctgcta agacgcccga gaatcgttgc
     5161 tgtctcagcg ggttggcagc ctgctgcgat gaagcacatc gatactgcac tgcacaagtg
     5221 cgcggcgaga cagtacggcg tcatcacgcg ctggcaactc ctcgcacacg gcaccaagga
     5281 tcagatcaag gtccggctca agtcgggtca gctcgagcgc cgctacgact gcgtgtacat
     5341 ctcgccgtcg gtaccgccaa gttgggagca ggacgagtta gcggcctgcg tcgccggtgg
     5401 acggccgaac ggcgtcgcgt cgctccgggc cgcggcaagg ctgtggggtc tgccgggtgg
     5461 agaagagatc gtcgaggtca ccacggcgcg acacaatcgc ctgcagtacg agggcgtgat
     5521 tgcgcatgag agccggtttc tcgaagaacg ggaccgcatg ctcctcaact cgattcccgt
     5581 cactcggccc gagcgaaccc tgctggatct ggccggcctc gtcgaactcc accgtctgcc
     5641 ggaacgcaca ctcgaactcg cgttgttcga ggccgtgcgt cgcgatctcg tcgacattcc
     5701 cagcgtatgg cgcgagtacg agcggctggg ccattacaag cgcctgggcg gcgaggtcat
     5761 ccttccaatc ctggagcggt tcgtcgcacc gatccgcaag cccgaaacga cgccggagac
     5821 cgaactgctg ttcatgctgc gagaggcggg gttccccgat ccggtcgctc aatattggct
     5881 cgtgctcccg aacggtgagc ggatccggct cgactacgcg tggccgtggt tgcggtgcgg
     5941 cgcggagttc gatccataca agttccacgg cgaccgggag cgatacgaga agaacgctgc
     6001 gcgcacccgt ttgatgcagg cgatgggctg gtcccgtgtc accgtgaccg acgatgacct
     6061 cgacgccggc attccgcaga cgatcatggc gctgcgcacg cttctcgccg cataacccca
     6121 cgtcggacgg gcgtcttaca agggcgaatt ccgcccttta gagacgcccg accaactcca
     6181 gcagggaggg gcggaagtcg gggagcaatg gaatgccggc ttggaccagc ttggcgttgg
     6241 ccaggaccga gtttgcgggt ctgggtgctg gcctcggggg gtcgagctcg gctgttgtga
     6301 tgggtttgac gcgctccggg tcttgattcg ttgcccgcag cacctcttgg gcgaactcgt
     6361 accagctgac tgcgccctgg ttggtgacgt gccagatgcc cgtgagccgt tggtcgacga
     6421 agcgcaccag catcgaggcc agatcggccg cgatcgtcgg atggcccacc tggtcgtcga
     6481 cgaacgtcag cgtctcgtgc tcgccggcca accggaggat cgtcttgacc atgttggcgc
     6541 cgtagcgacc gacgatccac gacgcgcgca cgaccgcgct cgtcgacacg tcaacctcga
     6601 tctcgcccgc caacttcgac tggccgtagg cgctctgcgg attcgtcgcg tcggtctcga
     6661 cgtacgggcc ctctttcaaa ccgtcgaaca cgtagtcggt cgagatggtg acgaggtacg
     6721 cgccgatgtc gaacgcggcc tgctgcacgt agcggttgcc catctggttg acggtgaacg
     6781 cggcatcgcg ctccgactcg cacttgtcga ccgcggtgaa cgcggcgcag tgcacgatcg
     6841 cgtcgggacg ctctcgcgac accactgcga gcacctgctc acgattcgcg acgtccaacg
     6901 acgaatgatc gaacgggatc gccgacgcac cgagcagctc gacgacctcg cgccccactt
     6961 ggccggccgc gccggtgacg aggacccggc tcacgattgc ttgagtggct cccaccacca
     7021 ctggttgtcg cggtaccagt gcacggtctc ggtgagggcc tcggcgaggt cacgttgcgg
     7081 tcgccacccg agcgcctcga ccttgtcggt cgcgatcgaa tagcggcggt cgtggccgag
     7141 ccggtcggcg acgtactcga tcatcgactc gtcggcatcg aggtgggcga ggatccggtc
     7201 ggtgagctcg cggttggaga tctcgttgcc accgccgatg ttgtagatct ccccgacggc
     7261 gccggtgcgc agcacgcgat cgatggcggc gcagttgtcc tccacgaagc accagtcgcg
     7321 cacgttcagg ccgtcgccgt agagggggac gcgcgtgccc tccaacagat tggtgacgaa
     7381 gagcgggatc accttctcgg gaaactggta cgggccgtac tggttcgacg agcgggtcac
     7441 gagcaccggc agcccgtagg tgctgtggta cgagagcgcg atgaggtcgg accccgcctt
     7501 cgacgccgaa tacggcgag
//
LOCUS       DASUGA010000079         2357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_537725, whole genome shotgun sequence.
ACCESSION   DASUGA010000079 DASUGA010000000
VERSION     DASUGA010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537725"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFX21_01455"
     CDS             <1..528
                     /locus_tag="VFX21_01455"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX5094643.1"
                     /translation="ARVDVTPSRPPRLDAAPEPAREKAPTRRTAPRVPTGTVHDSVAG
                     IEAMLATNGVLLVIDGYNVTKQAWGDESLEGQRQRLGVAVTALQRRAGCDVLCVFDGD
                     GTGHRPMLRRGGVRVMFSDAGEEADDVIVREVAALPKRVPIVVVSSDAWVREHVEAEG
                     AVVVGATAFLAALRR"
     gene            717..881
                     /locus_tag="VFX21_01460"
     CDS             717..881
                     /locus_tag="VFX21_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094644.1"
                     /translation="MAGTSACGDCIVSFIVSREPGDAVVVDAAEERALRALEEGGLVP
                     KLRHHPRRAV"
     gene            complement(947..2236)
                     /locus_tag="VFX21_01465"
     CDS             complement(947..2236)
                     /locus_tag="VFX21_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5094645.1"
                     /translation="MRVEDLVLVSVDDHVVEPPDLFEGRLTGEYAERAPKLVRKDTGI
                     DVWSFEGAELPNIGLNAVAGRPPEEYAMDPQSFDDMRTGCYDIHDRVKDMNANGILGS
                     MCFPSFPQFCGQLFARTAQHDPEFAEALVRAYNDWHIESWCGAYPGRFIPLSIPVIWD
                     PEKMAAEVRRVAEKGCHAVTFSENPVHLGHPSIHSDYWDPFFAACEELETVVCVHIGS
                     SSKMVITAPDAPMDVLITLSPVNIVQAAADFVWSRVPKKFPRLKYALSEGGIGWIPYF
                     RERIDYTYERHRFWTGQDMGDRRPTQVFDEQVVCCWIDDPVGIEMRHKMNIDMICWEC
                     DYPHSDSTWPQSPEQFMKQMQAADASDEDIDKISHQNAMRLFSYDPFAQLGGRDNCTV
                     GALREQAAGWDVSIVSRGIKATGTGAMDLLKMATGRP"
BASE COUNT          363 a          779 c          827 g          388 t
ORIGIN      
        1 gcgcgcgtcg acgtgacacc gagccgcccg cctcgcctcg acgcggcccc cgagcccgcg
       61 cgcgagaagg caccgacgcg gcgcacggcg ccgcgcgtgc cgaccggcac cgtgcacgac
      121 tcggtggccg ggatcgaagc gatgctcgcg acgaacggcg tgctgctcgt catcgacggc
      181 tacaacgtca cgaagcaggc gtggggcgac gagagtctcg aaggtcagcg acaacggctc
      241 ggtgtcgcgg tcactgcgct gcagcggcgt gccggttgcg acgtgctctg cgtcttcgac
      301 ggtgacggca cgggacaccg cccgatgctg cgccgcggtg gcgtgcgcgt gatgttctcc
      361 gatgccggcg aggaagccga cgacgtcatc gtgcgcgagg tcgccgcgct gcccaagcgg
      421 gtgccgatcg tcgtggtgtc gtccgatgcc tgggtgcgcg agcacgtgga agccgagggc
      481 gcggtcgtcg tcggggcgac cgccttcctc gcggcactgc gtaggtagcc gcgcggtcag
      541 tcgcacggcg cggcgccgga gcttgcggag gcgctcgcgg agtctcgagg ccggccaacg
      601 atccgagccg cctcacgcga gcaggtcgag agcgacaaag agagactgtc cgagctgtct
      661 gtcatgctgc gggcatggag cgtttgcccc tccgaatcga ctgtggagag tgcgtcatgg
      721 ccggcacgtc ggcctgtggc gactgcatcg tcagcttcat cgtgagccgt gaacccgggg
      781 atgccgtcgt cgtggacgcg gccgaggagc gggcgctgcg cgcgctcgaa gaagggggcc
      841 tcgtgccgaa gttgcggcac catccgcgac gagcggtctg accggcccgt gggcggagga
      901 acgcggccgc catgtccacc atggcggccg cgggcccacg ttgttgctac ggccggccgg
      961 tcgccatctt gaggaggtcc atcgcgcccg tgccggtggc cttgatgccg cggctcacga
     1021 tgctgacgtc ccatccggcc gcctgctcgc gcaatgcgcc gacggtgcag ttgtcgcggc
     1081 cgccgagctg cgcgaacggg tcgtacgaga acagtcgcat cgcgttctgg tgcgagatct
     1141 tgtcgatgtc ttcgtcggat gcgtcggcgg cttgcatctg cttcatgaac tgctcgggtg
     1201 actgcggcca cgtcgagtcg gagtgggggt agtcgcactc ccaacagatc atgtcgatgt
     1261 tcatcttgtg ccgcatctcg atgccgacgg ggtcgtcgat ccagcagcac acgacctgct
     1321 cgtcgaacac ctgcgtcggg cgacggtcgc ccatgtcctg gccggtccag aagcggtggc
     1381 gttcgtacgt gtagtcgatg cgctcgcgga agtagggaat ccagccgatc ccgccctcgc
     1441 tcaacgcgta cttcaaccgc gggaacttct tcggcacgcg cgaccacacg aagtcggccg
     1501 cggcctgcac gatgttcacc ggcgagagcg tgatgagcac gtccatcggc gcgtcgggcg
     1561 cggtgatgac catcttcgac gacgaaccga tgtgcacgca cacgacggtc tcgagctctt
     1621 cgcacgcggc gaagaacggg tcccagtagt cggagtggat cgacgggtgc ccgaggtgca
     1681 ccgggttctc ggagaacgtg accgcgtggc aacccttctc cgcgacgcgg cgcacttcgg
     1741 ccgccatctt ctcgggatcc cagatgacgg gaatcgacag cgggatgaag cggcccgggt
     1801 acgcgccgca ccacgactcg atgtgccagt cgttgtacgc gcgcacgagc gcttccgcga
     1861 attcggggtc gtgctgcgcg gtgcgcgcga agagctgtcc gcagaactgg gggaacgacg
     1921 ggaagcacat cgacccgagg atcccgttcg cgttcatgtc cttgacgcga tcgtggatgt
     1981 cgtagcaacc ggtccgcatg tcgtcgaacg actgcgggtc catcgcgtac tcctcgggcg
     2041 ggcgacccgc gaccgcgttc aacccgatgt tgggcagctc ggcgccttcg aacgaccaca
     2101 cgtcgatgcc ggtgtccttg cggaccagct tcggtgcccg ttccgcgtac tcgccggtga
     2161 gccggccctc gaacaggtcg ggtggctcga cgacgtggtc gtcgacgctc accaggacca
     2221 ggtcttcgac acgcatggac tgcccccttg aaacctgacg ctcacgtcag cataagtcag
     2281 accgaggtgg cgaggaatgc ctcgacttcc tggacgaacc gcttgccgct gaccgccccg
     2341 aggtggccct tgccctc
//
LOCUS       DASUGA010000080         3533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_954193, whole genome shotgun sequence.
ACCESSION   DASUGA010000080 DASUGA010000000
VERSION     DASUGA010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954193"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VFX21_01470"
     CDS             complement(<1..288)
                     /locus_tag="VFX21_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX5094646.1"
                     /translation="MDQQGVAQVRRFNRVVTQQVGALNDRFLGRDRPLGESRVLWEIG
                     PDGCDVRVLRERLDLDSGYLSRLLRSLETGGLVRVTTSASDARVRNVSLTAR"
     gene            complement(320..1855)
                     /locus_tag="VFX21_01475"
     CDS             complement(320..1855)
                     /locus_tag="VFX21_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX5094647.1"
                     /translation="MQGPRDVVVVLLDSLNRHMLGSYGGTEFETPNLDRLAGRSVRFT
                     NHHTGSLPCIPARHDLLVGALDFPWKPWGSIEVWEDAITGQLRHNPAVSTMLVSDHPH
                     LFETGGENYHTDFDAWDYVRGHEDDPWRTRADPSWIGGPTMPPVKSATNFRRGYDTSR
                     TWFRIETDFPGPRTMVAAATWLDRELTAERAPDERALLVVDEFDPHEPFDTPEPWATR
                     YDPDWEGERLIWPPYSVGGNVLSEREGRHLRSQYGAKLSMIDHWLGRILDVIDRNDAW
                     DTTAFVLCTDHGHYLGERDTWGKPAVAVHPEMGHIPLLVAWPGVAPRTCDALTTTVDI
                     HATLCDVFGVTPEHETHGVSLAPLLTGDTVSVREWALCGIWGREVHVADATRTYARAP
                     VETNRPLSMYSNRWSTMPVHALPGLRLPRPDARAALDRVGRSPVPVIRQPFDPSDHIP
                     FWAMGHFRGDLLYDRFEANATGAVRNQAADGPAAKEMTELLVEAMRAIAAPEEQLARL
                     GLG"
     gene            1938..>3533
                     /locus_tag="VFX21_01480"
     CDS             1938..>3533
                     /locus_tag="VFX21_01480"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX5094648.1"
                     /translation="MIDLREEAALDPALTGGKSANLARAALGGLSTLPGVVLTTAFSD
                     AIDGGIAVLDHPAVREAYTHAKGDEQDLVARSSSVVEDMANSSMAGQFDSVIGIHGYD
                     HFAKAVSQVLESRERANAADQPIAVLVQPLIDPAFGGIMFGIDPVSGREDRRVVSAIH
                     GGPEPLVSGEVNGSRYMLDESAKVLEFEQNDGAVLDRRHLRRLVALSNQVARVFGGPQ
                     DVEWAIASDGKLWLLQSRPVTTEIRGVPFGPVYGPGPVAETFPEPLSELECDLWVPPL
                     REAVREAVLLAGAATLADVNASEVVVTVDGHVAIDLRLAGEMPVKHSVANKLNPVPAA
                     RRLRGAWRVGRLRSAVPELAEHLLDRVDADLEAVPAMSDLTSRQLIALLHRSRIILRA
                     LHAHEILMGMLTDTGGNRMTGASVALRVLVEARRDGLTDEQVLARSPIVLALAPPRVA
                     PKPALPTEATAIHLGRDSQSSNDNGILREALRLRVRWVQELSGRAAWELGERLAMAGA
                     LTEPELIRHMTLEHVEAVATKRAV"
BASE COUNT          513 a         1208 c         1273 g          539 t
ORIGIN      
        1 gcgcgccgtc aacgacacgt tccgcacgcg cgcgtcgctc gcgctcgtcg tcacgcgcac
       61 cagcccgccg gtctcgagcg aacgcagcag ccggctcagg tagccggagt cgaggtcgag
      121 ccgttcgcgc aggacgcgca cgtcgcagcc gtcggggccg atctcccaca gcacccgtga
      181 ttcaccgagc gggcggtcgc ggccgaggaa ccggtcgttg agcgcgccga cctgctgggt
      241 cacgacccgg ttgaagcgcc ggacctgggc gacaccctgc tgatcgattc tctgaccgta
      301 gtcagagata aggtctcgat caaccgaggc cgaggcgggc gagttgctct tcgggtgcgg
      361 cgatcgcgcg catggcctcg acgagcagct cggtcatctc cttggccgcc ggtccgtcgg
      421 ccgcctggtt gcgcaccgcg cccgtcgcat tcgcctcgaa gcggtcgtac aagaggtcgc
      481 cgcggaagtg ccccatcgcc cagaacggga tgtggtcgct cggatcgaac ggctggcgga
      541 tgaccggcac cgggctgcgc cccacgcggt cgagcgcggc ccgcgcatcg gggcgaggga
      601 gccgtaggcc gggcagcgcg tgcaccggca tcgtcgacca ccggttcgag tacatcgata
      661 gcggccggtt cgtctcgacg ggcgctcgcg cgtaggtgcg cgtcgcgtcg gcaacgtgca
      721 cttcacgacc ccagatgccg cacaacgccc attcccgcac actgacggtg tcgccggtga
      781 gcagcggcgc gagcgacaca ccgtgcgtct cgtgctctgg tgtcacgccg aagacgtcgc
      841 acagcgtcgc gtggatgtcg accgtcgtcg tgagcgcgtc acaggtgcgc ggcgcgacac
      901 cgggccacgc gacgagcagc gggatgtggc ccatctccgg gtgtacggcg acggccggtt
      961 tgccccacgt gtcgcgctca ccgagatagt gaccgtgatc ggtgcacagc acgaacgcgg
     1021 tcgtgtccca cgcgtcgttg cggtcgatca cgtcgaggat gcgccccagc cagtggtcga
     1081 tcatcgagag cttggcgcca tactgcgacc gcaggtggcg tccctcgcgt tcgctcagca
     1141 cgttgccgcc gaccgagtac ggcggccaga tcaaccgttc gccttcccag tcggggtcgt
     1201 agcgcgtcgc ccacggttcg ggcgtgtcga acggttcgtg cgggtcgaat tcgtcgacga
     1261 cgagcagcgc gcgttcgtcg ggggcccgtt cggcggtcag ctcgcggtcg agccacgttg
     1321 ccgcggcgac catcgtgcgc gggccgggga aatcggtctc gatgcggaac cacgtgcgac
     1381 tcgtgtcgta gccgcggcgg aagttcgtcg ccgacttcac cggcggcatc gtcggtccgc
     1441 cgatccacga cgggtcggca cgggtgcgcc aggggtcgtc ttcgtgcccg cggacgtagt
     1501 cccacgcgtc gaagtcggtg tggtagttct cgccgccggt ctcgaacagg tgcgggtggt
     1561 cgctgaccag catcgtcgac accgcggggt tgtgccgcag ctgaccggtg atcgcgtctt
     1621 cccagacctc gatcgacccc cacggcttcc acgggaagtc gagcgcgccg acgaggaggt
     1681 cgtggcgagc cgggatgcag ggcagcgagc ccgtgtggtg gttcgtgaac cgcaccgagc
     1741 ggcccgccag ccggtcgagg ttcggcgtct cgaactcggt accgccgtaa ctgcccaaca
     1801 tgtgccggtt caggctgtcg aggagcacga cgacaacgtc acgcgggccc tgcatcgcgg
     1861 ccgacggtac atcgtgcatt gcggaacgtg tgcttcgttg tccagtctgt ggggcaatga
     1921 gtgacgatcg gcccctggtg atcgacctcc gcgaggaggc cgcgttggac cctgcgctga
     1981 cgggcgggaa gtcggccaac ctggcgcgcg ccgcgctcgg cggtctttcg acgctgcccg
     2041 gcgtcgtgct cacgaccgcc ttctccgacg cgatcgacgg cggcatcgcg gtcctcgacc
     2101 atcccgccgt gcgcgaggcg tatacccacg cgaagggtga cgagcaggac ctcgtggcgc
     2161 gcagctcgtc ggtcgtcgag gacatggcca actcgtcgat ggccggccag ttcgactccg
     2221 tcatcggtat ccacggctac gaccatttcg cgaaggcggt gtcgcaggtc ctcgagtcgc
     2281 gcgagcgcgc gaacgcggcg gaccaaccga tcgcggtgct cgtgcagccg ctcatcgatc
     2341 ccgcgttcgg cgggatcatg ttcgggatcg acccggtgtc cggacgcgag gaccggcgag
     2401 tcgtgagcgc gatccacggt ggccccgagc cactcgtcag cggcgaggtg aacggctcgc
     2461 gctacatgct cgacgagtcg gcgaaggtgc tcgagttcga acagaacgac ggcgcggtgc
     2521 tcgaccggcg gcatctgcgg cgactcgtcg cgttgtccaa ccaagtcgcg cgcgtcttcg
     2581 gcggtccgca agacgtcgag tgggcaatcg cttccgacgg gaagctgtgg ctcctgcaat
     2641 cgcgtccggt aacgaccgag atacgcggcg tgccgttcgg tccggtgtac ggccccggcc
     2701 cggttgccga gacgttcccc gagccgctga gcgagctcga gtgcgatctt tgggtcccgc
     2761 cgctgcgcga agcggtgcgc gaagcggtgt tgctcgcggg cgcggcgacc ctcgcggacg
     2821 tgaacgcgag cgaggtcgtg gtgacggtcg acgggcacgt ggcgatcgac ctgcggctcg
     2881 ccggtgagat gccggtcaag cacagcgtcg cgaacaagct gaacccggtc ccggccgcgc
     2941 gccggctgcg cggcgcgtgg cgggtcgggc gtttgcggtc ggccgtgccg gagctcgcgg
     3001 aacacctgct cgaccgcgtc gacgccgacc tcgaagcggt gcccgcgatg tcggatctca
     3061 cgagccgcca gctcatcgcg ctcctgcacc gcagccggat catcctccgc gcgctgcacg
     3121 cgcacgagat cctcatgggc atgctcacgg acaccggcgg caaccgcatg accggcgcgt
     3181 cggtcgcgtt gcgcgtgctc gtcgaagcgc gccgcgacgg cctgaccgac gaacaggtgc
     3241 tcgcgcggag tccgatcgtg ctcgcgctcg cgccaccgcg cgtcgcgccg aagcccgcgc
     3301 tgcccacgga ggcgaccgcg atccacctcg gccgcgactc gcagagcagc aacgacaacg
     3361 gcatcctgcg cgaggcgctg aggctgcgag tgcgctgggt ccaggagctc tcgggacgcg
     3421 cggcgtggga gctgggggag cggctggcga tggcgggtgc cctgaccgaa cccgagctga
     3481 tccgccacat gacgctggag cacgtcgagg cggtcgcgac gaagcgggcg gtc
//
LOCUS       DASUGA010000081         3311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_953993, whole genome shotgun sequence.
ACCESSION   DASUGA010000081 DASUGA010000000
VERSION     DASUGA010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953993"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VFX21_01485"
     CDS             complement(<1..371)
                     /locus_tag="VFX21_01485"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5094649.1"
                     /translation="MMNTTTDIDLGAIQVRFLVEAEESQGAATVFRCEVAPGAMVPGA
                     HSHDGFEETVYGLEGVMTFTVDGVTREVGPGDAMCIRRGQVHRFVNESGAPAAFLSVA
                     TPGIFGPDYFHDIRAVVTAGGP"
     gene            445..1092
                     /locus_tag="VFX21_01490"
     CDS             445..1092
                     /locus_tag="VFX21_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5094650.1"
                     /translation="MRDEKRPYDARRRRERAEEERTATRRKVVEAARKLFLEQGYVAT
                     TMSAIAREAGVAMQSVYSAGKSKADLLHLVVDLAVAGDHDDVMLVDRPEVAAMATEPS
                     ARRQVEILAERISATLTRLAPVWVASREAAAVDANAAANLEAAHRRRHETFAHMIRHI
                     REASLRESWAETTDTAWAIGSIDVFLLLQSRGWDAARYSAWIQDVLVTQLLAPGH"
     gene            complement(1089..2519)
                     /locus_tag="VFX21_01495"
     CDS             complement(1089..2519)
                     /locus_tag="VFX21_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HEX5094651.1"
                     /translation="MSRGRFIADWRRARLTVDERVARGKAARDAVPRATYGAWQPDAD
                     RPDPVALLEEQAEHRVPELVPIRYGRMLVSPFTFFRGAALIMASDLASTPRSNFTVQL
                     CGDAHMSNFGLFASPERRLVFDINDFDETLPGPWEWDVKRLAASFEVCGRDRAFAEAD
                     RRAITTAAVREYRKTMRQAAKMGSLEAWYDHLEVGALLEAVEAELRPKEARAAQRDIA
                     TARTRDSVRVLTKRVDNVDGDLRIRAEPPLIVPLEDLAASDIPDWEVFEPTIKKLLAS
                     YVKTLSHHHHPLEEFRYVHAARKVVGVGSVGTRCYILLLVGRDNDDPLILQVKEAGPS
                     VLERFLGPSEYENHGERVVAGQRLMQSASDIFLGWQRIKGVADGVPRDYYLRQLHDWK
                     GSFDSERLGVAGITLYARLCGATLARAHARWGDRIAIAWYLGKGDGFDDAVADFASAY
                     ADQNEADYEAFVAATSSGRLPIEAGL"
     gene            complement(2525..2911)
                     /locus_tag="VFX21_01500"
     CDS             complement(2525..2911)
                     /locus_tag="VFX21_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003937439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094652.1"
                     /translation="MARATLNVLASAVALIVASLILDDMSLDVDGFIIALLVFTVTGL
                     LIEPLLRQTAVRSAPALLGSTALVSTLISLVVTAIVSDGLRISGLATWIAATVLVWLI
                     ALAARFLLPFIIFRRTFQELRERRRD"
     gene            complement(2955..>3311)
                     /locus_tag="VFX21_01505"
     CDS             complement(2955..>3311)
                     /locus_tag="VFX21_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5094653.1"
                     /translation="DRVRSAMGSILETLAENPALARLTIVESAAVRPNGTSLLVGALG
                     ESFTMFVEVTPGLESPLTFDNLMKLVIGGIFTRLYFHILEGRTHELPELLEELVYFAL
                     LPFVGPEAAAAQYRSL"
BASE COUNT          540 a         1107 c         1163 g          501 t
ORIGIN      
        1 gggccgccgg cggtgacgac ggcgcggatg tcgtggaagt agtcgggccc gaagatgccc
       61 ggtgtcgcca ccgagaggaa cgcggccggc gcgccgctct cgttcacgaa ccggtgcacc
      121 tggcctcgcc ggatgcacat cgcgtcgccc ggaccgacct cgcgtgtcac gccgtcgacc
      181 gtgaaggtca tgacgccttc gaggccgtag accgtctcct cgaagccgtc gtggctgtgc
      241 gcgcccggga ccatcgcgcc cggcgcgacc tcgcaccgga agacggtggc ggcgccctgc
      301 gactcctcgg cttcgacgag gaaccggacc tggatggctc cgagatcgat gtcggtcgtt
      361 gtgttcatca ttgggcgttc tccttgcagg accattctgc ctagaactag attctgcgca
      421 gatcattgct ctagtgtcaa ccccatgcgc gacgaaaagc gtccgtacga cgcccgccgg
      481 cgccgggagc gcgccgagga ggagcgcacg gcgacccgcc ggaaggtcgt cgaggcggcg
      541 cgaaagctct tcctggaaca gggctatgtg gcgacgacga tgagcgcgat cgcgcgggag
      601 gccggcgttg cgatgcagtc ggtgtactcg gccggcaaga gcaaggccga cctcctccat
      661 ctggtggtcg acctcgcggt cgccggcgac cacgacgacg tcatgctcgt cgaccggccc
      721 gaggtcgcgg cgatggcgac cgagccgagc gcgcggcggc aggtcgagat cctggccgag
      781 cgaatctcgg cgacgctcac gcggctcgca ccagtatggg tcgcgtctcg cgaggcggcg
      841 gcggtcgacg cgaacgcggc cgccaatctc gaagccgcgc accggcggcg gcacgagacc
      901 ttcgcgcaca tgatccggca catccgcgag gccagcctgc gcgagtcctg ggcggaaacg
      961 accgacacgg cgtgggcgat cggcagcatc gacgtcttcc tgctgctgca gtcacgcggc
     1021 tgggatgccg cgcgctactc ggcatggatc caagacgtcc tcgtgacgca gttgctggcg
     1081 cccggtcatt agagcccggc ctcgatcggc aggcggccgg agctcgttgc cgcgacgaag
     1141 gcttcgtagt cggcctcgtt ctggtccgcg tacgccgatg cgaagtcggc gacggcgtcg
     1201 tcgaacccgt cgcccttccc caggtaccac gcgatcgcga tgcggtcacc ccaacgcgcg
     1261 tgcgcgcgag cgagggttgc gccacagaga cgcgcgtaga gcgtgatccc ggcaacgccg
     1321 agccgttccg agtcgaagct gcccttccag tcgtgcagtt ggcgcaggta gtagtcgcgc
     1381 ggcacgccgt cggcgacgcc cttgatgcgc tgccagccga ggaagatgtc gctggcggac
     1441 tgcatcagtc gttgtccggc gacgactcgt tcaccgtggt tctcgtactc gctcggtccg
     1501 aggaagcgtt cgagcaccga cgggccggct tccttcacct gcaagatcaa cggatcgtcg
     1561 ttgtcccggc cgacgagcag caggatgtag caccgcgttc cgacactgcc tacgccgacg
     1621 accttgcggg ccgcgtggac ataccggaac tcctcgagcg gatgatggtg gtgcgagagc
     1681 gtcttgacgt acgaggccaa cagcttcttg atcgtcggct cgaagacctc ccaatccggg
     1741 atgtcgctcg ccgcgaggtc ttcgagcggg acgatgaggg gtggttccgc gcgaatgcgg
     1801 aggtcgccgt cgacgttgtc gacgcgcttg gtgagcacac gcacgctgtc gcgtgtccgc
     1861 gcggtcgcga tgtcgcgctg cgccgcgcgc gcctctttgg gccggagctc ggcctcgacc
     1921 gcctcgagca gcgcgccgac ctcgaggtgg tcgtaccacg cctcgaggct tcccatcttc
     1981 gcggcctggc gcatcgtctt gcggtactcg cgcaccgccg ccgtcgtgat cgcgcgccgg
     2041 tcggcctcgg cgaacgcccg gtcgcgaccg cagacctcga agctggcggc cagccgcttc
     2101 acgtcccatt cccacggtcc ggggagcgtc tcgtcgaagt cgttgatgtc gaagaccaag
     2161 cggcgctcgg gtgacgcgaa gagtccgaag ttcgacatgt gcgcgtcgcc gcacaactgc
     2221 accgtgaagt tcgaccgcgg cgtcgacgcg aggtcggagg ccatgatcag ggccgcgccg
     2281 cggaagaagg tgaacggcga cacgagcatg cggccgtagc ggatcggcac cagttccggg
     2341 acgcggtgct ccgcctgttc ctcgaggagc gcgaccgggt cgggccggtc ggcgtccggt
     2401 tgccatgcgc cgtacgtcgc acgcgggacg gcgtcgcggg ccgccttccc gcgcgcgacg
     2461 cgttcgtcga ccgtcaggcg cgcccgacgc caatcggcaa tgaagcgccc gcgcgacacg
     2521 ggtgtcagtc gcggcgacgt tcgcgcaact cttggaaggt gcgccggaag atgatgaacg
     2581 gcaggaggaa gcgggcggcc agcgcgatca gccagacgag caccgtggcc gcgatccagg
     2641 tggcgaggcc cgagatccgc agcccgtcgg acacgatcgc ggtgacgacg aggctgatca
     2701 acgtcgacac cagggcagtg ctcccgagga gggccggcgc gctgcgcact gcggtctggc
     2761 gcagcagcgg ttcgatgagc aggcccgtca cggtgaacac cagcagcgca atgatgaatc
     2821 cgtcgacgtc caacgacatg tcgtcgagga tcaacgaggc gacgatcagc gcgacggcgc
     2881 tcgccagcac attcagggtt gccctcgcca cgagcctgat cattggcggg caggcttaca
     2941 cattccgcgt ccggtcacaa cgaccggtac tgcgcggcgg ccgcctcggg accgacgaac
     3001 ggcaacagcg cgaagtacac cagttcctcg agcagctccg gcagctcgtg cgtgcggcct
     3061 tcgaggatgt ggaagtagag ccgcgtgaag atgccgccga tgacgagctt catcaggttg
     3121 tcgaatgtga gtggcgactc gaggccgggc gtgacctcga cgaacatcgt gaacgactcg
     3181 ccgagggcgc cgaccaacaa actcgtgccg ttcgggcgga cggcggccga ctcgacgatg
     3241 gtcagacgcg cgagcgcggg attctcggcc aaggtctcga ggatcgaccc catcgcgctg
     3301 cggacgcgat c
//
LOCUS       DASUGA010000082         2197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_118118, whole genome shotgun sequence.
ACCESSION   DASUGA010000082 DASUGA010000000
VERSION     DASUGA010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118118"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="VFX21_01510"
     CDS             complement(<1..1097)
                     /locus_tag="VFX21_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX5094654.1"
                     /translation="MTFPDFSPTMGTFLRVGAARHGDRLLITAGGQRCTYAEADARSR
                     AVAKGLVADGVGKGTNVGILLPNSVDWAIAFYAVTRIGAVAVPINTFFKARELAWVLR
                     DADVAYLVSRSDFLGADYLALLESALPELAARGTDRPLLLRDAPFMRAISVWDERAAV
                     WSRGSESDLVARGAAAGISDDFLDEVEACVTPADRMVIVYSSGSTADPKGAIHTHGTV
                     VRHSMNVTDGYKVTRDDVMFSSMPFFWVGGLVTGLLAVQHHGAALVTMDAFEPGAALD
                     LLERERATIALGWPQQGKTMSEHPSFADRDLSSVVRTSLPDIVPPERRFPEIHPESLG
                     MTEACSSHLLGDPYVELGEERRGNFGQSVEGL"
     gene            1175..1906
                     /locus_tag="VFX21_01515"
     CDS             1175..1906
                     /locus_tag="VFX21_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5094655.1"
                     /translation="MARAATTQRTEAGSTAQPTAGSWKDRVVERSLGKATLRSLDRGH
                     AFIGAALVLLDRSNGDGFKVEDVAEEAGESVRCFYRHFASKDDLLLAVLEEAMERYAA
                     SITAVVEPFDEPLDRLAAALFGAVRLPHRNKPGVNVGLSRLHLKLAEVDPAKVGAARE
                     PVSFLMRSVVEEAARADVVDALAPEASAYMLMALTATHVLNQTLGNEFGLRMPTDEEH
                     VAFCLHGLNATLPSGWPERFRAVGQ"
     gene            1993..>2197
                     /locus_tag="VFX21_01520"
     CDS             1993..>2197
                     /locus_tag="VFX21_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HEX5094656.1"
                     /translation="MLTSSLAPPRYPGTYWGLVHGAARARPDHVLLTDDHGRSLTASE
                     LATAAERFAATLHAAGVRSGTVVS"
BASE COUNT          340 a          766 c          769 g          322 t
ORIGIN      
        1 agcccctcga ccgactggcc gaagttgccg cgccgttcct cgccgagctc gacgtacggg
       61 tcgccgagca ggtgcgacga acacgcctcg gtcatgccga gcgattcggg gtggatctcg
      121 gggaatcgcc gttccggcgg aacgatgtcc ggcaggctcg tgcgcacgac cgacgacagg
      181 tcacgatccg cgaaggaggg gtgctccgac atcgtcttgc cctgttgcgg ccacccgagc
      241 gcgatcgtcg cgcgctcccg ctcgaggagg tcgagcgcgg cgccgggctc gaacgcgtcc
      301 atggtgacga gcgcggcacc gtgatgctgc acggcgagca ggcccgtgac gaggccgccg
      361 acccagaaga acggcatcga cgagaacatc acatcatcgc gcgtcacctt gtagccgtcg
      421 gtgacgttca tcgagtgccg caccacggtg ccgtgcgtgt gaatggctcc cttggggtcg
      481 gccgtgctgc ctgagctgta cacgatcacc atccggtcag caggcgtgac gcacgcctcg
      541 acctcgtcca ggaagtcgtc gctgatcccc gccgcggcgc cccgcgccac gagatcggat
      601 tcggacccac gcgaccacac cgcggcgcgt tcgtcccaga ccgagatcgc gcgcatgaac
      661 ggcgcgtcgc gcaggagcaa gggtcgatcg gtgcctcgtg cagcgagctc cggcaacgcc
      721 gactcgagca gtgcgaggta atcggcgccg aggaagtcgg accgggagac gagatacgcg
      781 acatccgcgt cgcgcagcac ccacgcgagc tcgcgcgcct tgaagaacgt gttgatcggc
      841 acggccacgg ccccgatgcg agtgacggcg tagaacgcga ttgcccagtc gacactgttg
      901 ggcaggagga tccccacgtt ggtgcccttg cccacgccgt cggcgacgag tccctttgca
      961 acggcgcgcg accgcgcatc cgcttcggcg taggtgcacc gctgcccgcc ggcggtgatc
     1021 aggagacggt ccccatggcg cgcggcgccg acccgcaaga aggtgcccat ggtgggggag
     1081 aagtcgggga aggtcaccgc tccgaaacct ttcctcggcg cgtcgggggc tgcgcaacgg
     1141 catcgcgctc ggcgaacatg gatagtgtcg gcgtatggca cgagcggcca cgacccagcg
     1201 cacagaagcc ggatccaccg cgcaaccgac cgccgggtcg tggaaggacc gcgtcgtcga
     1261 gcgttcgctc ggcaaggcga cgctccgttc cctggatcgc ggccacgcgt tcatcggcgc
     1321 cgcgttggtg ctcctcgacc ggtcgaacgg cgatgggttc aaggtcgagg acgtcgccga
     1381 ggaggccggt gagtccgtgc ggtgcttcta ccggcacttc gccagcaagg acgacctgct
     1441 gctcgccgtc ctcgaggagg caatggagcg gtacgccgcc tcgatcacgg ccgtcgtgga
     1501 gccgttcgac gagccgctcg accgcctcgc cgcggcactg ttcggcgcgg tgcgcctccc
     1561 gcatcgcaac aagccgggcg tgaacgtcgg attgtcgcgg ctgcacctca agctcgccga
     1621 ggtcgacccg gccaaggtcg gggcggcgcg tgagccggtg tcgttcttga tgcgctcggt
     1681 cgtcgaggaa gcggcgcgcg ccgacgtggt cgacgcgctc gcgcccgaag cgtcggcgta
     1741 catgctcatg gccctcacgg ccacacacgt gctgaaccag accttgggca acgagttcgg
     1801 cttgcggatg ccgaccgacg aggagcacgt cgcgttctgc ctgcacggcc tcaacgccac
     1861 gctgccgtcg gggtggcccg aacggttccg cgccgtcggc cagtagcggc cgcgccgccg
     1921 cgatcgccgc gtcgcgctcc ggcgcgccgg tcgttgcaaa tgacgttggc atgcagcgaa
     1981 gatgctattt ccatgctgac gtcttcgctg gcgccgccgc gctatcccgg cacgtactgg
     2041 ggtctcgtgc acggcgccgc gcgagcgcgg cccgaccacg tcctgctgac cgacgaccat
     2101 ggacgctcgc tcacggcgtc ggagctcgcg acggcggccg agcgattcgc ggcgaccctg
     2161 cacgcggccg gagtgcggag cggcaccgtc gtctcct
//
LOCUS       DASUGA010000083         2101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_258813, whole genome shotgun sequence.
ACCESSION   DASUGA010000083 DASUGA010000000
VERSION     DASUGA010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258813"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="VFX21_01525"
     CDS             <1..1056
                     /locus_tag="VFX21_01525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX5094657.1"
                     /translation="PPPPPPPPPNSPPPAQAPAPAPAPQAAPPPAPAPPAPAPSNGDA
                     LVTSPVVRRLINEQGLDASSIHGTGEGGRITRNDVLDAARARGPAPATAPAAPASAPA
                     PQAAPAPVVRGQGDEVVPFDNIRRRTAEHMVRSLSTSAHVYTSVRVDFERVERVRRAH
                     QAEWKAEEGFALTYLPFIARAFCDVVHEYPHVNASVEGETLIVHRDVNLGIAIDLDFK
                     GLVAPVIRNADGKRLRLIAREVRDLATRAKSKQLSPDDISGGTFTITNMGPFGTSQTF
                     PIINQPQVAILATDGIRKEAVVVEGPDGDDAIVVHHVGFLSLAWDHRAFDGAYAASFL
                     NEVRNVLETRDWEAELA"
     gene            1058..>2101
                     /gene="lipB"
                     /locus_tag="VFX21_01530"
     CDS             1058..>2101
                     /gene="lipB"
                     /locus_tag="VFX21_01530"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX5094658.1"
                     /translation="MTLQARWLGTVPYREAEALQRALHERAAHDYLLLQEHPHVYTLG
                     SSAHLEHVLCDPASVGAEMVEADRGGDVTYHGPGQLVGYPIVSLAEWRAGQRDVVAYV
                     RRLEDVLIAVLADFGISAERSKGYTGVWVGDEKVAAIGVRVARGRTRHGFALNVDPDL
                     TMFEHIVPCGIRDRGVTSMSKLLGRAVPMRDVVDRVVTRFAESFDAPEMARYDVAWER
                     PEKPDLGIPITLTRKRPEWMKVRAHFDDGYLELKRLTKDLELHTVCEEAGCPNIYECW
                     ADRTATFMILGDRCTRACGFCLVDTRKPLAVDPEEPRRVAEAIEKLGLAHAVITCVAR
                     DDLPDGGAGVFAQT"
BASE COUNT          336 a          703 c          719 g          343 t
ORIGIN      
        1 cctccgccgc ctccgccgcc tccgcccaac tcgccgccgc ccgcgcaggc tccggcaccg
       61 gcaccggcgc cgcaggccgc gccgccgccg gcgcccgcgc cgccggcacc ggcaccgtcg
      121 aacggcgatg cgttggtgac gtcgccggtg gtgcggcggt tgatcaacga gcaagggctc
      181 gacgccagtt cgatccacgg cacaggtgaa ggtggtcgca ttacgcgcaa cgacgtcctc
      241 gacgccgcgc gcgctcgggg cccggctccg gcgaccgcgc cggctgcgcc cgcatcggca
      301 cccgcgcccc aagccgcacc ggctccggtt gtgcgcggtc agggcgacga agtcgtgccc
      361 ttcgacaaca tccggcgccg taccgccgaa cacatggtgc ggtccttgtc gacgagcgcg
      421 cacgtgtaca cgtccgtgcg cgtcgacttc gaacgggtcg aacgtgttcg gcgcgcgcac
      481 caagccgagt ggaaggccga agaaggattc gcgctgacgt acctgccgtt catcgcacga
      541 gcgttctgcg atgtggtgca cgagtacccg cacgtcaacg cgagcgtcga gggcgagacc
      601 ttgatcgtcc accgcgacgt caacctcggc atcgcgatcg atctcgactt caagggcttg
      661 gtcgcgccgg tgattcgcaa tgccgacggc aagcgccttc gcctcatcgc gcgtgaggtt
      721 cgcgacctgg cgacgcgcgc gaagtcgaag cagctgagtc ccgacgacat ttcgggcggc
      781 acgttcacga tcacgaacat gggtccgttc ggcaccagcc agacgttccc gatcatcaac
      841 cagcctcaag tggcgatctt ggcgaccgac ggcatccgca aggaagcggt cgtcgtcgaa
      901 ggtcccgatg gcgacgatgc catcgtcgtg caccacgtcg ggttcttgtc gctggcctgg
      961 gaccatcgcg cgttcgacgg tgcgtacgcc gcttcgttct tgaacgaggt gcgcaacgtg
     1021 ctggagacgc gtgattggga agcggaactc gcctgacatg accttgcagg cgcgctggct
     1081 cgggacggtc ccgtatcgag aagccgaagc gctgcagcgc gccttgcatg agcgcgcggc
     1141 ccacgactac ctgctgttgc aggagcatcc gcacgtgtac acgctcggtt cgagcgcaca
     1201 tctggaacac gtcctgtgtg atccggcgtc ggtcggcgcc gaaatggtcg aggcggatcg
     1261 cggcggcgac gtgacgtacc acggtccggg gcagctcgtc ggctacccga tcgtttcgct
     1321 cgcggagtgg cgggcggggc agcgcgacgt cgtcgcctac gtgcgccggt tggaagacgt
     1381 cttgatcgcg gtgctcgccg actttggtat cagcgcagag cgatccaagg gctacaccgg
     1441 tgtttgggtc ggcgacgaga aggtcgctgc catcggcgtg cgtgtggcgc gcggccgcac
     1501 gcgacacggg tttgcgctca acgtcgatcc cgacctcacg atgttcgagc acatcgtgcc
     1561 gtgcggcatt cgcgaccggg gcgtgacgtc gatgagcaag ctgctcgggc gcgcggtgcc
     1621 gatgcgcgac gtcgtcgatc gcgtggttac tcgcttcgcc gagtcgttcg acgcaccaga
     1681 gatggcgcgt tacgacgtcg cctgggagcg cccggagaag cccgacctcg ggattccgat
     1741 cacgctgacg cgcaagcggc ccgagtggat gaaggtgcgc gcccacttcg acgacggcta
     1801 cctcgaactc aagcggctca cgaaagatct cgaactgcac acggtgtgtg aggaagcggg
     1861 ctgtcccaac atctacgagt gctgggccga tcgcactgcc acgttcatga tcctcggcga
     1921 tcgttgcacg cgtgcgtgtg ggttctgcct cgtggatacg cgcaagcccc tcgccgtcga
     1981 tccggaagag ccgcgtcgag tcgcagaggc gatcgagaag ctcgggctgg cacatgcagt
     2041 gatcacctgt gtcgcgcgcg acgacttgcc cgacggcggc gcgggcgtgt tcgcgcagac
     2101 g
//
LOCUS       DASUGA010000084         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_953033, whole genome shotgun sequence.
ACCESSION   DASUGA010000084 DASUGA010000000
VERSION     DASUGA010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953033"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VFX21_01535"
     CDS             <1..697
                     /locus_tag="VFX21_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX5094659.1"
                     /translation="RNVSYALCNDRPTLLWFANQRAVEYHVTLVTADRPEHMTHLVLD
                     LDPPEGDGFAMAVRAAQLVRQVLTDVGLDGAVKTSGAKGVHVFIPVDDSVSMEDAAGA
                     TRAIAVRAEQLDPDLATTAFMKEDRGHKVFVDSTRVGGATVVSAYSPRVRPAVPVSFP
                     LAWGDLDGITTADFTIHTAVERLGDRDPWAELMPSPQRIPDELVEHGHTIPVPRVAAM
                     HEGKRRAREQRRQ"
     gene            707..1345
                     /locus_tag="VFX21_01540"
     CDS             707..1345
                     /locus_tag="VFX21_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094660.1"
                     /translation="MADWVTISSLATAGGTLVLAGATFSAVRSAHASTRVTERALLAS
                     IRPLLVPTRLEDPMEKIGFLDEHWIRAEGGRAVLEVTPQVIYMGFGLRNVGSGLAVLD
                     RWDLHAEVDGATPLRDASDFRRLSRDLYIAGGDQGFWQGALRDPDDPAFAAMRQAIEA
                     RSRVSVDVLYGDHEGGQRTVTRFALWPAGDDSWLTTTTRHWNLDQSAPRGDD"
     gene            complement(1358..1645)
                     /locus_tag="VFX21_01545"
     CDS             complement(1358..1645)
                     /locus_tag="VFX21_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HEX5094661.1"
                     /translation="MIRVSVLYPETEGATFDHDYYASTHVPMALKAWGLDGAEIDKGV
                     NGPYVAAVHFTFESLEAMQSAMASEGTGELMADMANFTTIQPVLQTSEKVS"
     gene            1751..>2220
                     /locus_tag="VFX21_01550"
     CDS             1751..>2220
                     /locus_tag="VFX21_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5094662.1"
                     /translation="MGVRRVVTGHTAEGKSIIASDTEVDAVPVGALGSSTTFVWHRDD
                     MAEFPDDGSLPPCEPGFPPPGGCGIAVLEIAPHTDEFDEFVRTALVPWCDPDEPGMHR
                     TATLDYDVVLEGTIGLQVDDGVEVTLHPGDIVVQNGTRHRWVNRGDGVARMLAVT"
BASE COUNT          334 a          784 c          754 g          348 t
ORIGIN      
        1 ccgcaacgtg tcgtacgcgc tctgcaacga ccgacccacg ctgctctggt tcgcgaacca
       61 gcgcgccgtc gagtaccacg tcacgctcgt gacggccgac cggcccgagc acatgaccca
      121 cctcgtgctc gacctcgacc caccggaggg cgatggtttc gccatggcgg tgcgcgccgc
      181 gcagctcgtg cgccaggtgt tgaccgacgt cggtctcgac ggcgcggtga agaccagcgg
      241 cgcgaaaggc gtccacgtct tcatccccgt cgatgactcg gtgtcgatgg aggatgcggc
      301 gggcgcgacg cgcgcgatcg cggtacgtgc cgagcagctc gatcccgacc tcgcaacgac
      361 ggcgttcatg aaggaggacc gcggtcacaa ggtgttcgtc gactcgacgc gcgtcggcgg
      421 tgcgaccgtc gtctcggcct acagcccgcg cgtccgtccg gccgtaccgg tgtcgttccc
      481 gctcgcgtgg ggcgacctcg acggcatcac gacggccgac ttcaccattc acaccgcggt
      541 cgagcgcctc ggcgaccgcg atccgtgggc cgagctgatg ccatcaccgc aacggatccc
      601 cgacgagctc gtcgagcacg ggcacaccat ccccgttccg cgcgtggccg cgatgcacga
      661 aggcaagcgg cgcgctcgcg aacagcgtcg tcagtagtgt cacgtcgtgg ctgactgggt
      721 cacgatctca tcgctcgcga cggccggcgg cacgttggtc ctcgcgggcg cgacgttctc
      781 cgcagtgcgc tctgcgcacg cgtcgacgcg cgtcaccgaa cgcgcgctgc tcgcgtcgat
      841 ccgaccgttg ttggtgccga cgcggctcga ggacccgatg gagaagatcg gcttcctcga
      901 cgagcactgg atccgcgccg aaggcggccg ggccgtgctc gaggtcacgc cccaggtgat
      961 ctacatgggc ttcgggctgc gcaacgtcgg cagtgggctc gccgtcctcg accgatggga
     1021 tctccacgcc gaggtcgacg gcgcgacacc gctccgcgac gcgagtgact tccggcgcct
     1081 cagtcgcgac ctctacatcg cgggtggcga ccaggggttc tggcagggcg cgctccgcga
     1141 ccccgacgat cccgcgttcg ctgcgatgcg ccaggcgatc gaggctcgga gccgggtctc
     1201 ggtcgacgtg ctctacggcg accacgaagg cggccagcgc accgtcacgc ggttcgcgtt
     1261 gtggcccgcg ggtgacgaca gctggctcac caccacgacg cgccactgga acctggacca
     1321 gtcggcgccg cgtggcgacg actgaactgc cggtcggtca ggagaccttc tcgctcgtct
     1381 ggagcaccgg ctggatcgtg gtgaagttgg ccatgtcggc catgagctcg cccgtgccct
     1441 ccgacgccat ggccgactgc atcgcctcga gcgactcgaa cgtgaagtgc accgcggcga
     1501 catacgggcc gttgacgccc ttgtcgatct cggccccgtc gaggccccac gccttcaacg
     1561 ccatcggcac gtgcgtgctc gcgtagtagt catggtcgaa ggtcgcaccc tcggtctcgg
     1621 gatacagcac gctgactcgg atcatgggag gtcctctcgt ggttggccgc gtcgtcgggg
     1681 ccgcgcaacc taacgcactt ccgtagtggt ccgatacgtt cctcatcgga acttcgaggg
     1741 agggcacgcc atgggtgtac gacgggtggt caccggccac acagccgagg ggaagtcgat
     1801 catcgccagc gacaccgagg tcgacgcggt gccggtcggg gcgctcggat cgagcacgac
     1861 gttcgtgtgg caccgcgacg acatggccga gttccccgac gacgggtcgc tcccgccctg
     1921 cgagcccggg ttcccgccac caggtggctg tggtatcgcg gtgctggaga tcgcgccgca
     1981 caccgacgag ttcgacgagt tcgtgcgcac tgcgctcgtg ccgtggtgtg atcccgacga
     2041 gccgggcatg caccgcaccg cgacgctcga ctacgacgtc gtactcgaag gcaccatcgg
     2101 cctccaggtc gacgacggcg tcgaggtgac gctccatccc ggcgacatcg tcgtccagaa
     2161 cggcacgcgg caccgctggg tgaaccgcgg cgacggcgtc gctcggatgc tcgccgtcac
//
LOCUS       DASUGA010000085         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_258467, whole genome shotgun sequence.
ACCESSION   DASUGA010000085 DASUGA010000000
VERSION     DASUGA010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258467"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2130)
                     /locus_tag="VFX21_01555"
     CDS             complement(<1..>2130)
                     /locus_tag="VFX21_01555"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX5094663.1"
                     /translation="ADGCEYECQQTGDERCDEIDNDCDGTTDEGIDTTTDRANCGGCG
                     NVCVFPNADASCEASTCVIGACRAGFVDLDGDDDNGCEYACVATGAETCNSVDDDCDT
                     TIDEGFVLDTDAMNCGRCGNACVFPNAVGTCTAGACGVASCLAGFHDIDGDPATGCEY
                     PCTPSGMADTCNGDDDDCDGRLDESDPDVGSACGTTTGECDPGVRACQLGSLTCVGGR
                     GPTSEVCNGRNDDCDARTDEAPLPGTGVRCGATNQGACEYGSVVCTAAALVCGGAYVG
                     PTTETCNGIDDDCNGATDDSPTPPGSPPPSCALTAGVCAGRTPTCGGGSGWSCAFPPT
                     YQSTEAICDTLDNDCDGTPDEGCLSVRPATDQRVDLGDTAGAANSVQPIVSGDGTTRV
                     YASWMDLRGGARAHVYFNRSTNSGNTWGAAPILLDTASGAAIGPRFGVVGAARDTINT
                     VWVDFRGGTSYREVWRRRSTDAGAGWGAGDTRINPSQNTDSFNVDVAVAGSRVYAVYE
                     NFTSSRSRHVFLVSSSDGGATFGGRLQVDNGTGATFVAATPKVAAVGTNVWVVWRDNR
                     NGALDVFVNRSTDGGATFGGTDRRLDVGTAAGSSSSFAPVVAAEAGNVYAAWVDDRGG
                     GVFDIWLNRSRDSGATWLSTDSIRLDADPLPHDSIEPRIAAPRSGTVVVAWVDYRFGF
                     PDVMVARSIDSAGTFTPPVRVDTGTGPG"
BASE COUNT          278 a          778 c          748 g          326 t
ORIGIN      
        1 tccggggccg gtgccggtgt cgacgcgcac gggcggcgtg aacgtgcccg cgctgtcgat
       61 gctcctcgcg accatcacgt ccgggaagcc gaagcggtag tcgacccacg ccacgacgac
      121 cgtgccggag cgcggcgccg cgatgcgcgg ctcgatcgag tcgtgcggga gcgggtcggc
      181 gtcgaggcga atcgagtccg tgctcagcca cgtggcgccc gagtctcggc tccggttgag
      241 ccagatgtcg aagacgccgc cgccgcggtc gtcgacccac gccgcgtaga cgttgcccgc
      301 ctcggccgcg accacgggcg cgaagctcga gctcgagccg gccgcggtgc cgacgtcgag
      361 gcgccgatcg gtcccgccga aggtcgcccc gccatcggtc gaccggttca cgaacacgtc
      421 gagcgcgccg ttccggttgt cgcgccagac gacccagacg ttcgtgccga ccgcggcgac
      481 cttcggcgtc gccgcgacga acgtcgcgcc cgtcccgttg tcgacctgca gtcggcctcc
      541 gaacgtcgct ccgccgtccg aggacgacac gaggaacacg tgccggctcc gggacgaggt
      601 gaagttctcg taaaccgcgt acacacggct gccagcgacg gcgacgtcga cgttgaacga
      661 gtcggtgttc tgcgacggat tgatccgcgt gtcgcccgcg ccccaacccg cgccggcgtc
      721 ggtgctgcgt cggcgccaga cctcgcgata cgacgtgccg ccgcggaagt cgacccacac
      781 cgtgttgatc gtgtcccgcg ccgcaccgac cacgccgaac cgcgggccga tcgccgcgcc
      841 gctcgccgtg tcgagcagga tcggcgcagc gccccacgtg ttgccgctgt tcgtcgagcg
      901 attgaagtag acgtgcgccc gcgcgccgcc ccggaggtcc atccacgacg cgtacacgcg
      961 tgtcgtgccg tcaccgctca cgatcggctg gaccgagttg gccgcgcccg ccgtgtcgcc
     1021 gaggtcgacg cgctggtcgg tcgccggacg gacggatagg catccttcgt cgggcgtccc
     1081 gtcgcagtcg ttgtcgagcg tgtcgcagat cgcttcggtg ctctggtacg tcggcgggaa
     1141 cgcgcacgac cagccggatc cgccgccaca cgtcggtgtg cggcccgcgc agacgcccgc
     1201 cgtgagcgcg cagctcggag gaggtgagcc aggaggcgtg ggcgagtcgt cggtcgcgcc
     1261 gttgcagtcg tcgtcgatcc cgttgcacgt ctcggtcgtc ggcccgacgt atgcgccgcc
     1321 gcacacgagc gcggccgccg tgcagacgac cgagccgtac tcgcaggcgc cctggttggt
     1381 cgcaccgcag cgcactccgg tgcccgggag cggagcttcg tccgtgcggg cgtcgcagtc
     1441 gtcgtttcgg ccgttgcaga cctcgctcgt gggcccgcgg ccgccgacgc acgtgagcga
     1501 tccgagctgg catgcgcgga caccggggtc gcactcgccc gtcgtcgtcc cgcacgcgct
     1561 gccgacgtcc ggatccgact cgtcgaggcg gccgtcgcag tcgtcgtcgt cgccgttgca
     1621 ggtatcggcc atcccgctcg gggtacacgg gtactcgcag ccggtcgctg gatcgccgtc
     1681 gatgtcgtgg aagccggcga ggcacgaggc gactccgcag gcgccggcgg tacacgtccc
     1741 gaccgcgttc gggaacacgc aggcgttgcc gcagcgcccg cagttcatcg catcggtgtc
     1801 gagcacgaag ccctcgtcga tggtggtgtc gcagtcgtca tcgacggagt tgcacgtttc
     1861 ggcgcccgtc gcgacgcagg cgtactcgca cccgttgtcg tcgtcgccat cgaggtcgac
     1921 gaacccggcg cgacacgcgc cgatgacgca cgtcgacgcc tcgcagctcg cgtcggcgtt
     1981 cgggaacacg cagacgttgc cgcagccgcc gcagttcgcg cggtcggtcg tcgtgtcgat
     2041 cccctcgtcg gtggtcccat cgcagtcgtt gtcgatttcg tcacagcgct cgtcaccggt
     2101 ctgctggcac tcgtactcgc agccgtccgc
//
LOCUS       DASUGA010000086         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1229311, whole genome shotgun sequence.
ACCESSION   DASUGA010000086 DASUGA010000000
VERSION     DASUGA010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229311"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VFX21_01560"
     CDS             <1..957
                     /locus_tag="VFX21_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094664.1"
                     /translation="GQGAQIVFANAATMSLSGDLVIPCGRTSGSGAPIAITTLQADVV
                     SPGGTSYLLTPTGGTSSSNPPFVDPGNITTYADNVNATATNVGKHKIVLSTTGFNAQS
                     VTDGSTLTLHARHSESKNSDVSVTVTAGDGSTCPKTLTSYTSMTDDAVVLDCPAFHEV
                     EPFTVSYTAQPNTKDTNLALDGLQITGTSASTIPKLAGCAVHTALVKGTCALISPPSN
                     GKGRIDVNAVVYIPQSTITGKFSSTGHLQIGVGVIAWALDLAINPNIGSAATIGTASH
                     TYTDGDVRLTASIDGSTWIDSRVQYPNGGTTTPVITSWDIQR"
     gene            985..2058
                     /locus_tag="VFX21_01565"
     CDS             985..2058
                     /locus_tag="VFX21_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX5094665.1"
                     /translation="MSIDHSPIQSYLDAMIELGASDLMLTANTPTRARVDGQLRPLPG
                     ERALTDEEVEQRILTMLSPGLREEFREKQELDFSFSYDVSHRFRGNCFFQRGVPALSL
                     RALPTEIPDFESLHLPPVCEYFCNLPQGLVLVTGPTGSGKSTTLASMIDFINTNRECH
                     ILTIEDPIEFVHQHKTAVVNQRQVGDDTSSFAGALRAALREDPDVILVGEMRDPETIQ
                     FALTLAETGHLVFATLHTNDAAQSLDRVWDVFPHQRQAQVRVQLSASLAGIISQRLVR
                     RTTTGVVAAFEVLVATPAVRNIIREGNTHQLRNIIHGGRAHGMITLEASLAERIDAGL
                     ISIDEALTHTLYPNEVDELRRSA"
     gene            complement(2059..>2414)
                     /locus_tag="VFX21_01570"
     CDS             complement(2059..>2414)
                     /locus_tag="VFX21_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094666.1"
                     /translation="PPPPPPPPVDVATPAPVYVEPEATYEIESGPLDPEVQALVDDLY
                     RKARAEIVGSFDGMESELVPEAPPTHEEPPREQPPVDAPRRRGWVPAVLADRRRHDGT
                     GPGKRPNGNGWRRLD"
BASE COUNT          422 a          834 c          767 g          391 t
ORIGIN      
        1 gggcaggggg cgcagatcgt cttcgccaac gccgcgacga tgagcctctc gggcgacctc
       61 gtgatcccgt gcgggcgcac gagcggatcc ggcgcaccga ttgcgatcac gacactgcag
      121 gccgacgtcg tgagcccggg cggcacgtcg tacctcctca cgccgaccgg cggtacgagc
      181 tcctccaatc cgccgttcgt cgatcccggc aacatcacga cgtatgccga caacgtcaac
      241 gcgacggcaa cgaacgtcgg gaagcacaag atcgtcctgt cgaccaccgg cttcaacgcg
      301 cagtcggtca ccgacggttc aacgctcacg ttgcatgccc ggcacagtga atcgaagaat
      361 tccgacgttt cggtgacggt caccgccggt gacgggtcga cgtgcccgaa gacgcttacg
      421 tcttacacct cgatgaccga cgacgcggtc gtcctcgatt gcccggcctt ccacgaggtg
      481 gagccgttca cggtctccta caccgcgcag cccaacacga aagacacgaa cctcgcgctc
      541 gacggactgc agatcaccgg cacgtccgcc tccacgatcc cgaagctcgc cgggtgcgcg
      601 gtgcacacag cgctggtgaa ggggacctgt gcgctgatct caccgccgtc gaacggcaag
      661 ggccggatcg acgtcaacgc ggtcgtgtac atcccgcaga gcacgatcac cggcaagttc
      721 tccagcaccg gtcacctcca aatcggtgtg ggcgtgattg cgtgggcgct cgatctggcg
      781 atcaacccga atatcggcag cgcggcgacc atcgggacgg catctcacac gtacaccgac
      841 ggtgacgtcc gcttgaccgc atccatcgac gggtccacgt ggatcgattc gcgtgtgcaa
      901 tatccgaacg gcggtacgac gacgccggtc atcacatctt gggacatcca acggtgaaag
      961 cagagaggac acaacgtgtc gcgcttgtcg attgaccatt cgccgatcca gtcgtatctc
     1021 gacgcgatga tcgagctcgg tgcgagcgac ctgatgttga ccgcgaacac gccgacgcgt
     1081 gctcgtgtcg atggacagct gcggccgctc ccgggtgaac gagcactcac cgacgaggaa
     1141 gtcgagcagc gcatcctgac gatgctgtcg cccgggctgc gggaggagtt ccgcgagaag
     1201 caggagctcg acttctcgtt ctcgtacgac gtctcgcatc ggttccgcgg caactgcttc
     1261 ttccaacgcg gcgtgcccgc gctctcactc cgggcgctgc cgaccgagat cccggacttc
     1321 gagtcgctgc acttgccgcc cgtctgtgaa tatttctgta atctgccgca gggtctggtg
     1381 ctcgtcaccg ggccgacggg ctccggcaag tcgacgacgc tggcgtcgat gatcgacttc
     1441 atcaacacga accgtgagtg ccacatcctg accatcgagg acccgatcga gttcgtacat
     1501 cagcacaaga cggcggtggt caatcagcgc caggtcggcg acgacacgag ctccttcgcc
     1561 ggcgcgttgc gcgcggcgtt gcgcgaagac cccgatgtca tcctcgtcgg cgagatgcgc
     1621 gaccccgaga cgatccagtt cgcgttgact cttgccgaga ccggccacct cgtcttcgcg
     1681 acgctgcaca ccaacgacgc cgcacagtcc ctcgaccgcg tctgggacgt gttcccacac
     1741 cagcgccagg cgcaagtgcg cgtgcagctc tccgcatctc tcgccggcat catctcgcag
     1801 cgccttgtgc ggcggacgac caccggcgtt gtcgcggcat tcgaagtgct cgtcgccaca
     1861 cccgccgtgc gcaacatcat ccgcgagggc aacacacacc agttgcgaaa catcatccac
     1921 ggtgggcgcg cccacgggat gatcacgctc gaagcgtccc tcgccgagcg catcgatgcg
     1981 ggactcatca gcatcgacga ggcgctgacg cacacgttgt acccgaacga ggtggacgag
     2041 ctgcgccgca gtgcgtagtc agtcgaggcg gcgccagccg ttgccattgg ggcgcttgcc
     2101 cggaccggtg ccgtcgtggc gccggcggtc ggcgagtacg gccggcaccc aaccgcgccg
     2161 gcgcggtgcg tccaccggcg gctgctcgcg cggcggttcc tcgtgcgtgg gcggcgcctc
     2221 gggcacgagc tccgattcca tgccgtcgaa ggagccgacg atctccgcgc gcgccttgcg
     2281 gtacaggtcg tcgacgaggg cctgcacttc ggggtcgagc ggaccggact cgatctcgta
     2341 cgtcgcttcg ggctcgacgt acaccggtgc gggcgtcgcg acgtcgaccg gcggcggcgg
     2401 cggcggcggc ggcg
//
LOCUS       DASUGA010000087         2255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1368196, whole genome shotgun sequence.
ACCESSION   DASUGA010000087 DASUGA010000000
VERSION     DASUGA010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368196"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..1670
                     /locus_tag="VFX21_01575"
     CDS             255..1670
                     /locus_tag="VFX21_01575"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEX5094667.1"
                     /translation="MVSRLRTRWALIGAVIALTIGLSGCIIGPITGPGKDSLESSFDV
                     SKVGYQRAEYFFSETAVSYGNTAALGTDGKWAAAPDPATPAGAFSTRFLVYRPTDMSK
                     FNGTVVVEWMNVSAGTDIPINWAVAHNEFVRAGAIYVGVTAQSVGVNAIKTANPDRYG
                     KLSHPGDSYSYDIFTKVGQELQKAPKRFFGGAVAARYIAVGESQSAGRLVTYINAIHP
                     LVDVYDGFMVHSRGAGSSSLRQGPLAPVPTPSPALIRNDLDVPVMVVEAEGDVIGSNT
                     QIRQADSPLFREWEMAGTSHADQYIVITNSSDIGDGQGEIQMLNGLRNPLAVGCGLPI
                     NAGGHHWIMQAAWHHLDAWVRDHTVTPPSGPLLQVASTSPTVLSRDSHGNALGGVRSP
                     QVDAPVATITSFNTGPGFCLLFGSTTPFTAEELAALYPTHADFVNAWKASTTANVAAG
                     FLLPADKTLLDAAAEASTIPN"
     gene            1704..>2255
                     /locus_tag="VFX21_01580"
     CDS             1704..>2255
                     /locus_tag="VFX21_01580"
                     /inference="COORDINATES: protein motif:HMM:NF027900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HEX5094668.1"
                     /translation="MATVLALVLIGAVAGPMDAVAARQPRAERVAQWSRFESTYVPPA
                     TPTNPFDPEEIDVRAEFSGPGGVQREALGFWFQDYERSLVNGRERLTPVGQPGFRIRF
                     TPERAGQWRWRWVIRTPGHVDVQPWQRLRVSHGRDRGFLRVSERDPRMLAFDDGSPYF
                     AIGENTGWYDQRGTYAIDDWFGQL"
BASE COUNT          365 a          788 c          738 g          364 t
ORIGIN      
        1 cgaacgtctg cacctccacc cagggcgcca gctccggctt ccagtgccgg ccgtcgacct
       61 cggccgcgag cgtctcccag aagtccggcg cgctcacgtc ctcctgcagc acaccgccat
      121 catcccggcc cgacgccgat cagtgccaac gggcccgcgg ccggggcctc gcagcgtcgg
      181 ctttacgggg cccgggtcga gcatctaaga acatggtcgt tccttgcggg tgggcacgat
      241 ccgggggaag tcgaatggtg tcgcgactcc gcacgcggtg ggctctcatt ggcgcggtca
      301 tcgcgctgac gatcgggttg tcgggttgca tcatcgggcc gatcaccggt cccggcaagg
      361 attcgctcga gtcctcgttc gacgtctcga aggtcggcta ccagcgcgcc gagtacttct
      421 tcagcgagac cgcggtgagc tacggcaaca ccgccgcgct cggcacggac ggcaagtggg
      481 ccgcggcacc ggatcctgcg accccggccg gcgcgttttc gacccggttc ctcgtctacc
      541 gcccgaccga catgtcgaag ttcaacggca ctgttgtcgt cgagtggatg aacgtcagcg
      601 ccggcaccga tatccccatc aactgggcag tcgcacacaa cgaattcgtg cgcgcgggcg
      661 ccatctacgt cggcgtcacc gcacagtcgg tcggcgtcaa cgccatcaag accgcgaacc
      721 cggatcgcta cggcaagctc tcgcaccccg gtgacagcta ctcgtacgac atcttcacca
      781 aggtcggaca ggagctgcag aaggcgccga aacggttctt cggcggcgcg gtggccgccc
      841 gctacatcgc ggtcggcgag tcgcagtcgg caggtcgcct ggtcacgtat atcaatgcaa
      901 tccaccctct cgtcgacgtc tacgacggct tcatggtgca cagccgcggc gccggctcct
      961 catcgctgcg gcagggccca ctcgcgcccg tgccgacacc atcaccggcg ctgatccgca
     1021 acgacctcga cgttccggtg atggtcgtcg aggccgaagg cgacgtcatc ggttcgaaca
     1081 cgcagatccg gcaggccgac tcaccgctgt tccgtgaatg ggagatggcc ggcacgtcgc
     1141 acgcggacca gtacatcgtg atcacgaaca gcagcgacat cggcgacggt cagggcgaga
     1201 tccagatgct caacggattg cgcaacccgc tcgctgtcgg ttgcgggctc ccgatcaacg
     1261 cgggcgggca ccactggatc atgcaagcgg cgtggcacca cctcgacgcg tgggtgcgcg
     1321 atcacacggt caccccgccg tcgggaccgc tgctgcaagt cgcgtcgacg tcgccgacgg
     1381 tgttgtcgcg tgactcgcac ggcaacgcgc tcggcggggt ccgttcgccg caggtcgacg
     1441 cgccggtcgc gacgatcacc agcttcaaca ccggccccgg cttctgtctt ctgttcggca
     1501 gcacgacgcc gttcaccgcc gaagagctgg ccgcgctcta cccgacgcac gccgacttcg
     1561 tgaacgcgtg gaaggcatcg acgacggcga acgtggcggc cggcttcctg ttgccggcgg
     1621 acaagacatt gttggacgcg gccgccgagg cctcgacgat cccgaactag cgcgccggaa
     1681 tctcgctcgt gcgacgctgt ccgttggcaa cggttctcgc gctggtgctc atcggtgcgg
     1741 tggccggtcc gatggatgcg gtcgcggcgc gtcagccccg agccgagcgc gtcgcgcaat
     1801 ggtcacggtt cgagtcgacc tatgtgccgc cggcgacacc gaccaacccg ttcgatcccg
     1861 aggagatcga tgtacgggcg gagttctccg gcccgggcgg cgtacaacga gaggcgctcg
     1921 gcttctggtt ccaggactac gagcgcagcc tggtcaacgg ccgcgagcgg ttgacgcccg
     1981 tcggtcaacc gggcttccgg atccggttca cgcccgagcg tgccggtcaa tggcggtggc
     2041 ggtgggtcat ccgcacgccg ggccacgtcg acgtgcagcc gtggcaacgc ctccgcgtgt
     2101 cgcacgggcg cgatcgtggc ttcctgcggg tcagcgaacg cgacccgcgc atgctcgcgt
     2161 tcgacgacgg cagtccatac ttcgcgatcg gtgagaacac aggctggtac gaccaacgcg
     2221 gcacctacgc aatcgacgac tggttcggtc aactc
//
LOCUS       DASUGA010000088         6286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1368037, whole genome shotgun sequence.
ACCESSION   DASUGA010000088 DASUGA010000000
VERSION     DASUGA010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368037"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VFX21_01585"
     CDS             complement(<1..590)
                     /locus_tag="VFX21_01585"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013424452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX5094669.1"
                     /translation="MNDHELASVAAQRAGDFLLELRARGGTGAEGDRQADDLILPLLR
                     EHRPDDAILSEESKDDPSRLDHSRVWIVDPLDGTREYGEAGRADWAVHVALVIDEVPG
                     PSAVALPAQGLVLSTAPPPALAPAVDGPIRILVSRTRPPALATRLAELLGGELVPMGS
                     AGAKTMAVVQGLADVYAHGGGQYEWDSAAPVGVATAA"
     gene            complement(587..967)
                     /locus_tag="VFX21_01590"
     CDS             complement(587..967)
                     /locus_tag="VFX21_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5094670.1"
                     /translation="MTKILVAADAKWVRDQVRAAFVAPGQEVVEVTRGQDVRDTFVRE
                     NPDLVILDLQIGNMGGIACALDLRLEESGGRAPHATILLLLDREADKFLAKRVDVDAL
                     LVKPVDAGVLRRAARQLLEAGAPV"
     gene            complement(980..2101)
                     /locus_tag="VFX21_01595"
     CDS             complement(980..2101)
                     /locus_tag="VFX21_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEX5094671.1"
                     /translation="MTLASLVADRRVVICCGSGGVGKTTTAAVIALEGARAGRNACVV
                     TIDPARRLASALGLESLSDTPSEIDRARWDGTGGPGRLSALMLDTKSTFDHLVRSNAV
                     SEEQAQRILDNAFYRNVSGALGGTQEYMAMEKLHELHESGGFDLIVVDTPPTRHALDF
                     LDAPQRLTRLLDNRIFRLLMMPTRAGLRVASVAVQAFLRTVAKIVGSEVIEDVVAFFR
                     AFEGMEDGFRERAQVVERLLADPETAFVLVTSPRRDALEEAHFFADRLAQNGQHIEAL
                     VVNRVHPTFGEESPDGLRATVGALEANPGADAAAAARIAALYDNLADFREIADRERAY
                     LDGLRHRIGSAHVVFVPYLAHDVYDFEALHAVGQALLAD"
     gene            complement(2098..3042)
                     /locus_tag="VFX21_01600"
     CDS             complement(2098..3042)
                     /locus_tag="VFX21_01600"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEX5094672.1"
                     /translation="MEVAQFCSTSHVVIVAGKGGVGKTTVTAALAVAATRAGLRVLVV
                     EVEGKSGLSAMFGTDALGYDESELEPGLRARFLTPDAALVEYLHTHGLKRISKRLVSS
                     GALDVVANAVPGMKDILVLGKVKQLDEAKVADLIIVDAPAAGHAITFLCSARGLLDAV
                     KVGPIQKQAADVVALLSDPARCQVMLVTMPEETPVSEAIETAFAVEDRAGVALAPIVV
                     NGCHAPLPAGVVASADAITRDAAAIGRFVSPRETADLVAAAAFREERYVIQEEQVERL
                     AQRLPLAQLRLPFVFTPDIGRSDLDVLADALEAGIAAL"
     gene            3063..4451
                     /locus_tag="VFX21_01605"
     CDS             3063..4451
                     /locus_tag="VFX21_01605"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX5094673.1"
                     /translation="MNRTNPPSLVRLLLALVCVAAGALLVAGPVGAADDEPATDAGID
                     VVQVNGLIDPSNAALIKKVVREAQEAGSSLVIVQLDGTGAVDVDVESLAQVIDDATIP
                     VGVWVGPSGSNARGASGYLALHATVVAVSQGSGIGPVHPLRFHDTSAPSRDELIAQLS
                     AVQRAHGRDVASVPTVVDERLSANRAQELGVIDMVEPTVGDFIVRLDGKTVTVQGEDI
                     KLETADVIGEGADAQLQPNQTVRFHKLDLTQQLAHTFNTPWVAYAMFVIGFALIIFEF
                     FTAGIGIAGFVGACAVVGACFGFSHLPVQGWAIFLLLFGLFGLSIDLQAGGLGPWTFI
                     GSASLVAGSIWLYGGSSRLDPSWWVLVIVCGGTVLFMLSGMTAMVRSRFSTPTVGRED
                     LIGETGIAEVDVAPDGVVRVRDALWKARTNRATPARAGEEIRVVEVMGVVLEVEPASG
                     GARDYRDRSRGH"
     gene            4527..4790
                     /locus_tag="VFX21_01610"
     CDS             4527..4790
                     /locus_tag="VFX21_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX5094674.1"
                     /translation="MAVGIIHETWHLKAACRGPESMLFFPPSFSERREEREAREEKAK
                     AICLGCPVRQDCLDFAVRVREPHGIWGGLTETERRRLLPRDDN"
     gene            complement(4787..>6286)
                     /locus_tag="VFX21_01615"
     CDS             complement(4787..>6286)
                     /locus_tag="VFX21_01615"
                     /inference="COORDINATES: protein motif:HMM:NF020672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HEX5094675.1"
                     /translation="HVAILELLGIRHGVVAISKADTVDDETLELAALELEERLPGRPA
                     AAWPVVAVDSLSGRGLDELRAALDAVLAAAPAPRDVGRPRLWVDRVFSARGSGTIVTG
                     TLTGGALHVDHDVIVEPGHRSARVRRIESQHAQLEGIGPGSRVALNLSGVDHHDITRG
                     DAVVRPEHWAVVSTVDVALQPLPGTEFPHGGTVDAHVGSGDHVARLSMLDDESQLARL
                     RFDTALPLAPGDRIVLRSSARQSTIAGAEVLDVAPARRAADARTRRGLPLSGRVLAAR
                     PWATTGDFVALAGVADGDTYSAQLVDEGLAVRVGSWVLAPEHAHDLRERARALVTTRD
                     PSTPGVTLAALASALGIDASQLRALLAGDDALAVERDLVREAGASRVESSAEAQALLA
                     ALDEKPFEPPSPAGLGAAPGLVRALVGAGVLVDLDGVVLTANAFASARALVVDALREN
                     EAMTVAALRDVLGTSRKYALPILNRLDAEGVTRRRGDDRVLGPRAEQLD"
BASE COUNT          947 a         2201 c         2207 g          931 t
ORIGIN      
        1 gccgcggtcg cgacgccgac gggcgccgcg gaatcccact cgtactgacc gccgccgtgt
       61 gcgtacacgt cggcgagtcc ttgcacgacg gccatggtct tcgcgcccgc cgagcccatc
      121 ggcaccaact cgccgccgag caactccgcg agccgcgtcg cgagtgccgg cggccgcgtg
      181 cgcgacacca agatgcggat cgggccgtcg accgcgggcg cgagcgcggg tggcggcgcc
      241 gtcgagagca cgaggccctg cgcggggagc gcgactgcgg acggaccggg cacttcgtcg
      301 atcacgagcg cgacgtgtac cgcccagtcg gcgcggcctg cttcgccgta ctcgcgcgtg
      361 ccgtcgagtg gatcgacgat ccacacgcgt gagtggtcga gtcgtgaagg atcgtccttc
      421 gactcctccg acaagatcgc gtcgtcgggc cggtgctcgc gcagcagcgg caagatgaga
      481 tcgtcggcct gccggtcacc ttccgcgccg gtgccgccgc gagcgcgcaa ctcgagcagg
      541 aagtcaccgg cgcgctgcgc cgcgacggaa gcgagctcgt ggtcgttcac accggagcgc
      601 cggcttcgag cagctgccgt gccgcccgcc gcaggactcc cgcatcaact ggtttcacga
      661 gcagcgcgtc gacgtcgacc cgcttggcca agaacttgtc ggcttcccga tccagcagca
      721 acaagatcgt ggcgtgcggc gcccgaccgc ccgactcctc gagccgcaga tcgagcgcgc
      781 acgcaatgcc acccatgttg ccgatctgca ggtcgaggat caccagatcg gggttctcgc
      841 gcacgaacgt gtcgcgaacg tcttgccccc gggtgacttc gacgacctct tggccgggcg
      901 cgacgaacgc ggcccgcact tggtcgcgca cccacttcgc atccgcggcg acgaggatct
      961 tggtcatcgg gcgagaaccc tagtcggcca gtagcgcctg gccgacagcg tggagtgctt
     1021 cgaagtcgta gacgtcgtgg gcgagatagg ggacgaacac gacatgtgcc gatccgatgc
     1081 ggtgccggag cccgtcgaga tacgcgcgct cacgatccgc gatctcgcgg aagtcggcga
     1141 ggttgtcgta gagcgccgcg atgcgggccg cggccgcggc gtcggcaccg ggattcgctt
     1201 ccagcgcccc gaccgttgcg cgcaagccgt cgggagactc ctcgccgaac gtcggatgca
     1261 cgcggttcac gacgagcgct tcgatgtgct ggccgttctg tgcaaggcga tcggcgaaga
     1321 agtgcgcctc ttcgagcgcg tcgcgccgcg gcgaggtgac gagcacgaac gcggtctccg
     1381 gatcggcgag cagacgttcg accacttgtg cgcgctcgcg gaagccgtcc tccattccct
     1441 cgaacgcgcg gaagaacgcc acgacgtcct cgatcacttc ggagccgacg atcttcgcca
     1501 ccgtgcgcag gaacgcctgc accgcgacgc tcgcaacccg caacccggcg cgggtcggca
     1561 tcatcaacag gcggaagatc cgattgtcga ggagtcgtgt caaccgctgc ggtgcgtcga
     1621 ggaagtcgag cgcgtggcgc gtcgggggag tatcgacgac gatcaggtcg aagccgccgc
     1681 tctcgtgcag ctcgtgcagc ttctccattg ccatgtactc ctgcgtgccg ccgagcgcgc
     1741 cggagacgtt gcgatagaag gcgttgtcga ggatccgctg cgcttgttcc tccgacaccg
     1801 cattgctgcg cacgagatgg tcgaaggtcg acttggtgtc gagcatcagc gcggacaggc
     1861 ggcccgggcc accggtgccg tcccaccggg cccggtcgat ctcgctcggc gtatccgaga
     1921 gcgactccag cccgagcgcg ctggccagcc ggcgcgcggg atcgatggtg acgacacagg
     1981 cgttacgtcc cgcgcgtgcg ccctccaggg cgatcaccgc ggcagtcgtc gtcttgccga
     2041 ctccgccgct gccgcagcag atgacgacgc gccggtcggc gacgagcgaa gcaagcgtca
     2101 cagtgccgcg atccccgctt cgagcgcgtc ggcaagcaca tcgagatccg agcgtccgat
     2161 gtcgggagtg aacacgaacg gcagccgcag ctgcgcgagt ggcaaccgct gcgcgagccg
     2221 ttcgacttgt tcttcttgga tcacgtagcg ctcctcgcgg aacgcggccg cggcgacgag
     2281 gtccgcggtc tcgcgcggcg acacgaaccg gccgattgcg gcggcgtccc gcgtgatcgc
     2341 gtcggccgac gcaacgacac cggcgggcaa cggcgcgtgg catccgttga cgacgatcgg
     2401 cgcgagcgcg actccggcgc ggtcctcgac cgcgaacgcg gtctcgatcg cttcgctcac
     2461 cggcgtttcc tccggcatcg tcacgagcat cacctggcag cgcgcgggat cggagagcag
     2521 cgcgacgaca tcggcggctt gcttctggat cgggccgacc ttcaccgcgt cgagcagccc
     2581 ccgcgccgag cacaggaagg tgatcgcgtg gcccgccgcc ggcgcgtcga cgatgatgag
     2641 gtctgcgacc ttggcctcgt cgagctgctt caccttgccg agcacgagga tgtccttcat
     2701 gcccggcacc gcgttggcga ccacgtcgag cgcgcccgag ctcaccagcc gcttcgagat
     2761 ccgcttcagc ccgtgggtgt ggagatattc gacgagcgcg gcgtccgggg tcaggaaccg
     2821 ggctcgcagg ccgggttcca gctccgactc gtcgtagccg agcgcgtcgg taccgaacat
     2881 ggccgagagc cccgatttgc cctcgacttc cacgacaagc accctgagac cggcgcgagt
     2941 ggcagcgacc gcgagcgcgg ccgtgacagt cgtcttcccg acgccgccct tacccgcgac
     3001 gatcacgacg tgggaggtgg agcagaactg cgcgacctcc atcacttcat gctacgagag
     3061 ccgtgaatcg aacgaacccc ccgagcctgg ttcgcctgct actggcgctg gtgtgcgtcg
     3121 ccgcgggcgc actgttggtg gcggggcccg tgggcgcggc cgacgacgag cccgcgaccg
     3181 acgccgggat cgacgtcgtc caggtgaacg gcctcatcga tccgagcaat gcggcactca
     3241 tcaagaaggt cgtgcgcgag gcgcaggaag ccggctcgtc actcgtgatc gtgcaactcg
     3301 acggtaccgg cgcggtcgac gtcgacgtcg agtcgctcgc gcaggtcatc gatgacgcca
     3361 cgatccccgt cggcgtgtgg gtcggtccgt cggggtcgaa cgcacgcggt gcgagcggct
     3421 acctcgcgtt gcacgcgacc gtggttgcgg tctcgcaggg ctcgggcatc ggcccggtgc
     3481 atcccttacg gttccatgac accagcgccc ccagccgcga tgagctgatt gcccagctca
     3541 gcgccgtgca gcgcgcgcac ggacgcgatg tcgcatcggt cccgaccgtc gtggacgaac
     3601 ggctctcggc caatcgcgcg caggagctcg gcgtcatcga catggtggag ccgaccgtcg
     3661 gcgacttcat cgtgcgcctg gacggcaaga ccgtgaccgt gcagggcgaa gacatcaagc
     3721 tcgaaaccgc ggacgtcatc ggtgaaggcg cggacgcgca attgcaaccg aaccagacgg
     3781 tgcgcttcca caagctcgac ctcacccaac agctcgcgca cacgttcaac acgccgtggg
     3841 tcgcgtacgc gatgttcgtc atcggcttcg cgctcatcat cttcgagttc ttcacggccg
     3901 gcatcggcat cgcgggcttc gtgggtgcgt gcgcggtcgt gggtgcgtgc ttcgggttct
     3961 cacacctgcc ggtgcagggc tgggccatct tcctgttgtt gttcgggctt ttcggcctct
     4021 cgatcgacct ccaggccggc ggcctcgggc cctggacgtt catcgggagc gcgtcgctcg
     4081 tcgcgggatc gatctggctc tacggcggtt cgtcgcgcct cgacccgtcg tggtgggtgc
     4141 tcgtcatcgt gtgcggcggc accgtgctgt tcatgctctc gggcatgacc gcgatggtgc
     4201 gctcgcgatt ctcgacaccg accgtcggtc gcgaagacct gatcggtgaa acgggcatcg
     4261 ccgaagtcga cgtcgcgccc gacggtgtcg tgcgcgtccg cgacgcgttg tggaaggcgc
     4321 gcacgaatcg cgcaacaccc gcgcgcgccg gcgaggagat ccgtgtcgtc gaggtgatgg
     4381 gcgtcgtcct cgaggtcgag cccgcgtcgg gcggcgcgcg cgactaccgc gaccgatctc
     4441 gcggccactg atcaggcctt ttgtccaccg cgagcaccgg cgctacgttc cgcgccgtcc
     4501 agcggggccg gaggggggac acgaccatgg ccgtgggcat catccacgag acgtggcatc
     4561 tcaaggcggc ctgccgaggc ccggaatcga tgctgttctt cccgccgtcg ttctccgaac
     4621 ggcgtgaaga gcgagaagcg cgcgaggaaa aggcgaaggc catctgcctc gggtgccccg
     4681 tacgccagga ctgcctcgat ttcgcagtgc gcgtccggga gccgcacggc atctggggtg
     4741 gcctgaccga gaccgagcgc cgccgcctgc tgccgcgcga cgacaactaa tcgagctgct
     4801 ccgcccgcgg gcccagcacg cggtcgtcac cccggcggcg cgtcacaccc tcggcatcga
     4861 gacgattgag gatcggcagc gcatacttgc gcgacgtgcc gaggacgtcg cgcagtgccg
     4921 cgacggtcat cgcttcgttc tcgcgcaatg cgtcgacgac gagcgcgcgg gcggacgcga
     4981 atgcattcgc cgtgagcacg acgccgtcca ggtcgaccag cacgcccgcg ccgacgaggg
     5041 cccgcacgag tcccggtgcc gcaccgagcc ccgccggcga cggcggctcg aacggcttct
     5101 cgtccagcgc ggcgagcagc gcctgggcct cggcgctcga ttcgacgcgt gatgcgccgg
     5161 cctcacggac gaggtcgcgc tcgacggcga gggcgtcgtc gccggcgagc agcgcgcgca
     5221 gctgcgacgc atcgatgccg agcgccgacg cgagcgcggc gagcgtgaca cccggcgtcg
     5281 aggggtcccg cgtggtcacg agtgctcgcg cccgttcccg gaggtcgtgc gcgtgctccg
     5341 gtgccaggac ccacgacccg acgcgcacgg cgagcccctc gtcgacgagc tgtgcactgt
     5401 aggtgtctcc gtccgcgaca ccggccagcg cgacgaagtc gccggtcgtg gcccagggtc
     5461 gggccgcgag cactcggccc gagagaggaa gtccacgacg ggtccgtgcg tcggcggcgc
     5521 gtcgcgcggg ggcgacgtcg agcacttcgg cgcccgcgat cgtcgactga cgcgccgacg
     5581 accgcaacac gatgcggtcg ccgggtgcga gtggcaacgc ggtgtcgaaa cggaggcgcg
     5641 cgagctggct ctcgtcgtcg agcatgctga gtcgcgcgac atggtcaccg gagccgacgt
     5701 gtgcgtcgac ggtaccgccg tgtgggaact cggtgcccgg caacggctgc agcgcgacgt
     5761 cgacggtcga gaccaccgcc cagtgctcgg gccgcacgac cgcgtcgccg cgggtgatgt
     5821 cgtgatggtc gacgccgctg aggttcagcg caacccggct gcccggtccg atgccttcga
     5881 gctgcgcgtg ctgcgactcg atgcgccgga cccgtgcgct ccggtgaccg ggctcgacga
     5941 tcacgtcgtg gtcgacgtgc agcgctccgc cggtgagcgt gccggtcacg atggtccccg
     6001 aaccgcgcgc cgagaagaca cggtcgaccc acagccgcgg ccggccgacg tcgcgcggcg
     6061 ccggtgccgc ggcgaggacg gcgtcgagcg cggcgcgcag ctcgtcgagc ccgcggccgg
     6121 acagcgaatc gacagcgacc accggccagg ccgccgccgg ccggccgggc agccgttcct
     6181 cgagctccag cgccgcgagt tcgagcgtct cgtcgtcgac cgtgtccgcc ttggagatcg
     6241 cgacgacgcc gtgccggatc ccgagcagct cgaggatcgc cacatg
//
LOCUS       DASUGA010000089         2819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_257558, whole genome shotgun sequence.
ACCESSION   DASUGA010000089 DASUGA010000000
VERSION     DASUGA010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257558"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..556
                     /locus_tag="VFX21_01620"
     CDS             80..556
                     /locus_tag="VFX21_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX5094676.1"
                     /translation="MEHEMVVEIPQGSRNKYEMDHDSGQIWLDRTLFTAMSYPYDYGF
                     FPDTLADDGDPLDALVLLMQPTFPGCHVWVRPVAVFWMQDEKGGDAKVLCVPAHDPRW
                     DHVRDKDDVASYLLAEIEHFFDFYKTIEPGKSTSTRGWEGADAAEREIAASKARYV"
     gene            complement(547..1371)
                     /locus_tag="VFX21_01625"
     CDS             complement(547..1371)
                     /locus_tag="VFX21_01625"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5094677.1"
                     /translation="MDVLDLWVNLVTPQSAAHFLGQAENAHIPGYLGGDGSTGVGIDQ
                     LLGVMDSLGVQTGVLTPGIVGNGVEQSLEIADAHAGRFLVAAVVVDPGKPTKNVRRIR
                     EYAHHERFCMVRVAPLTSQVPTNDAKHYPVYQACEELGVPVAINVGIPGPRVRSRVQH
                     PELLEDVMIDFPDLVIIGAHMGHPYEELLMNYMRKWPNLYLSCTAYAPRYMDPNLVQF
                     MNTKTYRGRVIWGSDEPWFPMTRSLSEAKSLPLDDDAMALFLGGTARRLLTRSSQT"
     gene            1439..1930
                     /locus_tag="VFX21_01630"
     CDS             1439..1930
                     /locus_tag="VFX21_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094678.1"
                     /translation="MEIALPTKLQNYVQAQVRAGKYATPDEVVSDAVRRMQEADVEAS
                     AGIVSDAIKMASQAQRDLLSLVQRADEERNVMQQIMGVAGNAVDASRGLARKVPVARE
                     VERVVSGSLEQVSNLTKQGELEAKQMRQGLEATARMLGLLAATLEKVNGATSALNRNG
                     SSS"
     gene            1989..>2819
                     /locus_tag="VFX21_01635"
     CDS             1989..>2819
                     /locus_tag="VFX21_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX5094679.1"
                     /translation="MRARWFGVVALAVALPAFPAPTAAAAEAPDGPIVAQIVGGGDVD
                     VSTAPWIVALVWAGGGNGASLRCGGTRVAPEWVLTAAHCVNGHAPADFDVVGGVTDLR
                     TVTAEDREHISQTFVHPAYDAGDNTSDVALLRLAVPDDAAPALALNADPATPARGEAL
                     DTYGWGRLSTNGQSSAVLQGVVLQEMTDDSGNCGAFGNRYIPAHHVCAGVPGGGRDAC
                     YGDSGGPLVASAPDAPVLVGVTSWGQECALADYPGVWARVSTYTNWITTTMTTPPPPV
                     R"
BASE COUNT          476 a          929 c          986 g          428 t
ORIGIN      
        1 ctgagctcga cgcgctccca ggaggcgctc gaggtgagat gggccgtcac acgacgacca
       61 tatcggcgac aatgtggtca tggagcacga gatggtcgtc gagatccccc agggctcgcg
      121 caacaagtac gagatggacc acgactccgg acagatctgg ctcgaccgca ccctcttcac
      181 cgcgatgtcg tatccgtacg actacgggtt cttccccgac acgctcgccg acgacggcga
      241 tccgctcgac gcgctggtgc tcctaatgca gccgacgttc ccgggatgtc atgtgtgggt
      301 gcgacccgtc gcggtgttct ggatgcaaga cgagaagggt ggcgacgcga aggtgctgtg
      361 cgtccccgcg cacgatcccc gctgggacca cgtacgggac aaggacgacg ttgcgtcgta
      421 tctcctcgcc gagatcgagc acttcttcga cttctacaag acgatcgagc cgggcaagag
      481 cacgagcacg cgcggctggg aaggcgccga cgccgccgaa cgtgagatcg ccgcgtcgaa
      541 agcgcgctac gtctgagacg aacgtgtcag aaggcggcgg gcggtgccgc ccaggaacaa
      601 cgccatcgcg tcgtcgtcga gcggcagcga cttcgcttcg ctcagcgagc gcgtcatcgg
      661 gaaccacggt tcgtcgctgc cccagatcac ccgtccgcgg tacgtcttgg tgttcatgaa
      721 ctgcacgagg ttcgggtcca tgtacctcgg cgcgtatgcg gtgcacgaca ggtagaggtt
      781 cggccacttg cgcatgtagt tcatgagcaa ctcctcgtag gggtggccca tgtgcgcgcc
      841 gatgatgacg agatcgggga agtcgatcat cacgtcttcg agcagctcgg ggtgctgcac
      901 gcgcgaacgc acgcgcggac ccgggatgcc gacgttgatc gcaacgggca caccgagctc
      961 ttcgcacgcc tggtagacgg ggtagtgctt ggcgtcgttg gtcggcacct gcgaggtcag
     1021 cggcgcgacc cgcaccatgc agaaccgttc gtggtgcgcg tactcgcgta tacggcggac
     1081 gttcttggtc ggcttgcccg gatccacgac gacagccgcg acgaggaagc gcccggcgtg
     1141 cgcgtcggcg atctcgagtg actgctcgac gccgttgccc acgatccccg gcgtgagcac
     1201 gccggtctgc acgccgagcg agtccatgac gcccaggagc tgatcgatgc cgacgccggt
     1261 gctgccgtcg ccgccgaggt atccggggat gtgcgcgttc tcggcctgac cgaggaaatg
     1321 ggcggccgat tgcggggtga cgaggttcac ccagaggtcg agaacgtcca cgaagctgac
     1381 ggtaatgtca gtcgtgtcgg ggtgccaacc acaccagtcg tcggccggga gagtggtgat
     1441 ggagatcgcg ctgcccacga agctgcagaa ctacgtgcag gcgcaggtgc gtgcgggcaa
     1501 atacgccacc cccgacgaag tcgtgagcga cgcggttcgc cgtatgcaag aggccgacgt
     1561 cgaagcatcg gccggcatcg tgagcgatgc gatcaagatg gcgtcgcaag cacaacgcga
     1621 tctgctctcg ctcgtacagc gcgcggacga agagcgcaac gtcatgcagc agatcatggg
     1681 cgtcgccggc aacgcggtcg acgcgtcacg cggcctggcg cgcaaggtcc ccgtcgcacg
     1741 cgaagtcgag cgggtcgtga gcggttcgct cgaacaggtc tcgaacctga ccaagcaggg
     1801 tgagctcgag gccaagcaga tgcgccaggg cctggaagcg accgcgagga tgctcggcct
     1861 cctggccgca acgctcgaaa aggtgaacgg cgcaacctcc gcattgaacc ggaacgggtc
     1921 ctcgtcctaa cttctgggca tctgggccga acggcccggg ccgaacggcc cagtggggag
     1981 tggggaacat gcgagctcgt tggtttggtg tggtcgcgct cgcggtcgcc cttcccgcgt
     2041 ttcccgcgcc gacggccgcg gccgccgagg cgccggacgg tccgatcgtg gctcagatcg
     2101 tcggcggcgg cgacgtcgac gtgagcacgg cgccctggat cgtggccctg gtctgggcgg
     2161 gtggcgggaa cggcgcgtcc ctgcgctgcg gtggcacgcg cgtcgcgccc gagtgggtgc
     2221 tcaccgccgc gcactgcgtc aacgggcacg caccggccga cttcgacgtc gtcggtggcg
     2281 tcaccgatct gcgtaccgtg accgcggagg accgcgagca catcagccag acgttcgtgc
     2341 accccgccta cgacgccggc gacaacacat ccgacgtcgc gctgctccgg ctcgcagtgc
     2401 ccgacgacgc cgcgccggcg ctcgcgctca acgcggatcc cgcgacaccg gcgcgcggcg
     2461 aggcgctcga cacgtacggc tggggccggc tcagcaccaa cggacagtcc tccgcggtgt
     2521 tgcaaggtgt ggtgctgcag gagatgaccg acgactcggg caactgcggc gcgttcggca
     2581 accgctacat ccccgcgcac cacgtctgcg cgggcgtgcc cggcggcggc cgcgacgcgt
     2641 gctacggcga cagtggaggg ccgctcgtcg cgagcgcgcc cgacgcgccc gtgctcgtcg
     2701 gtgtgacgag ttggggccag gagtgcgcgc tcgccgacta tcccggtgtc tgggcacgcg
     2761 tctcgacgta cacgaactgg atcacgacca cgatgacgac acccccgccg cccgtccgg
//
LOCUS       DASUGA010000090         3398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2062722, whole genome shotgun sequence.
ACCESSION   DASUGA010000090 DASUGA010000000
VERSION     DASUGA010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2062722"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="VFX21_01640"
     CDS             complement(<1..1093)
                     /locus_tag="VFX21_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5094680.1"
                     /translation="MPRGEPRRHRALGLRRRGTIAFAALALLVSASMAILTYQIARTY
                     LVDKRESLALRQAALNARAVQEVLRARDPDVQDLMAGLPTSSTSHPVLRIGGEWYAAA
                     VSFGQDIVPSALLQMTEDDRAGRQLTTANGEPVLVVGVPIAGTDAVYFEAFSLTELER
                     TLATLRNSLLIAAAITTVIGAFLGSYLNRRALKPLRTFADGAAGLARGRLETRLAAPG
                     DPDLAPVAAAFNDMAAALQDRIEREARFASDVTHELRTPLTALSAAVEVLDRRADERM
                     RPAVDVVQAQVQHFERLVLDLLEISRYDAGAAELTTEAVDVARFFHSLTDSLDAEGVS
                     LVIDPSTPKTFDLDKRRVERALANLLENAH"
     gene            complement(1080..1787)
                     /locus_tag="VFX21_01645"
     CDS             complement(1080..1787)
                     /locus_tag="VFX21_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013734240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5094681.1"
                     /translation="MPRLLVIEDEAPVREMMRFLLEDEGFAIDEAATGEAGLEQFADG
                     HHDLVLVDLRLPGIHGFDVCRAVRRDSDVPIIIVTAQVDSHDVVAGLEAGADDYVTKP
                     FVAKELTARIRAALRRSTATEPGAGSPLRFGAIEIAPAEGVVRKDGVTVHLTRTEFRL
                     LCEFAEHAGIVLSREQLLERIWGYDYLGDSRLVDAHVRRLRSKIEDDPADPVWLQTVR
                     GLGYKIRRPDDAHDAAR"
     gene            2023..2394
                     /locus_tag="VFX21_01650"
     CDS             2023..2394
                     /locus_tag="VFX21_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094682.1"
                     /translation="MGQEGGVVRWGVPMRLGRRYRAAADARVLGQTCLVELVMGSSPM
                     VRHVFTVTGAGRCDSYVNCATSVPSRWRSFVDTRALMQSRPRVISGLAQRAEWLESAP
                     DTSYVAPRRSYGSAVAGRRYR"
     gene            2391..2690
                     /locus_tag="VFX21_01655"
     CDS             2391..2690
                     /locus_tag="VFX21_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094683.1"
                     /translation="MTTDVAASAAPLEQLPIARELEGGFFVVTTDSTMWLVDTRQRRF
                     QRHGHDVAVQDALTFGTWQPYERAQWQAPDTFLIVPTRSRVLIRCEKARVCRPRL"
     gene            complement(2713..>3398)
                     /locus_tag="VFX21_01660"
     CDS             complement(2713..>3398)
                     /locus_tag="VFX21_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094684.1"
                     /translation="LESIAIADDTLSLVTGPATVTQLDAKTLDVAGSIDLGVAGAIDT
                     TVAASGTDLYAVTQTPSEWVVTLLRNSAVERRATVAGSDAPVAVAADDAHLWIVTATS
                     VTTLSTTALDDPATISAPRDATPLHSAAAANGAVWFTGGNAHDLYALHANDAVPTVIP
                     LVERTGTADRIPVSVVAGRDAVFALVQASDDPQQHDVVIAKIVPASEKVTHRVALPSD
                     LFVGAIAVS"
BASE COUNT          508 a         1128 c         1216 g          546 t
ORIGIN      
        1 ggtgcgcatt ctccagcagg ttcgccaacg cgcgttcgac gcgccgcttg tcgaggtcga
       61 acgtcttggg agtcgaaggg tcgatgacga gggagacgcc ttccgcatcg aggctgtccg
      121 tcagtgagtg gaagaagcgc gcgacgtcga ctgcctcggt cgtgagctca gccgctcccg
      181 cgtcgtagcg cgagatctcg aggaggtcga gcacgaggcg ctcgaagtgt tgtacctgcg
      241 cctgcacgac gtcgacggcc gggcgcatgc gctcgtcggc gcgccggtcg agcacttcga
      301 ccgccgcgga caatgcggtg agcggcgtgc ggagctcgtg cgtcacgtcg gatgcgaagc
      361 gcgcctcgcg ctcgatccga tcctgcagcg cggccgccat gtcgttgaac gccgcggcga
      421 caggtgcgag atcaggatcg cccggcgccg cgagtcgcgt ttcgagccgg ccgcgggcga
      481 ggccggctgc cccatcggcg aacgtgcgca gcggcttcag tgcccgtcgg ttgagatagg
      541 agccgaggaa cgcgccgatc accgtcgtga tcgcggccgc gatgagcaac gagttgcgca
      601 gcgtggccag ggtgcgctcc aactcggtga gcgagaaagc ttcgaaatag acggcgtcgg
      661 tacccgcgat cggcacgcca acgacgagga cgggttctcc gttggcagtc gtcagctgac
      721 ggccggcgcg gtcgtcttcg gtcatctgca acaacgcgga tggcacgatg tcctgcccga
      781 agctcaccgc ggcggcgtac cactcgccac cgatgcgcaa cacgggatgg ctggtgctgc
      841 tcgtcgggag tccggccatg agatcctgga cgtccgggtc acgcgcgcgc agcacctcct
      901 gcaccgcgcg tgcgttgagc gcggcctgcc gcagcgccag cgattctcgc ttgtcgacca
      961 gatacgtgcg ggcaatctgg tacgtcagga tcgccatcga tgccgacacg agcaacgcca
     1021 atgccgcgaa cgcgatcgtg cctcggcgcc ggaggccgag cgcgcggtgc cgccgcggct
     1081 caccgcgcgg catcgtgtgc gtcgtcgggc cggcggatct tgtaccccag gccgcggacg
     1141 gtctgcagcc agacgggatc ggcgggatcg tcttcgatct tcgagcgcag gcggcgcaca
     1201 tgcgcgtcga cgaggcggct gtcgccgagg tagtcgtagc cccagatgcg ttcgagaagt
     1261 tgttcgcgtg acaagacgat gccggcatgc tcggcgaact cacagagcag acggaactcc
     1321 gtgcgcgtca ggtgcacggt cacgccgtct ttgcgcacga cgccttccgc cggcgcgatc
     1381 tcgatggcgc cgaagcgcaa cggcgagccg gcgcccggct cggtggcggt cgatcgtcgc
     1441 agcgcggcac gaatgcgcgc ggtcagctct ttggcgacga acggcttggt gacgtagtcg
     1501 tcggcgccgg cctcgagccc cgcgacgacg tcgtggctgt cgacctgcgc ggtgacgatg
     1561 atgatcggga cgtcgctgtc gcgccggacg gcgcggcata cgtcgaaacc gtggatcccc
     1621 gggaggcgaa ggtcgaccaa cacgagatcg tggtggccgt cggcgaactg ttccaggccg
     1681 gcctcgcccg tggccgcttc gtcgatggcg aagccttcgt cctcgagcag gaagcgcatc
     1741 atctcgcgca ccggcgcctc gtcctcgatg acgagcaggc ggggcatggg cccatgatgc
     1801 ccccgggccg gccgggcccg ggcccgaacc cgggcccgcg ggccgattgt cgcgaaatca
     1861 caacatgaag cgcgccccgt cgtcgcgggc gacccgcaag gtggcgccat gcagcctgtg
     1921 agttgccacc tccttcaggc tggcgtccat gtccgggcgc cggaggatcg tgtcgtctac
     1981 gtcctgcgga cgttgtccga atacgaccga tggtgcccgg acgtgggcca agagggcggt
     2041 gtcgtccgct ggggcgtgcc gatgcggctc ggccgccggt accgcgccgc cgccgacgcc
     2101 cgggtgctgg gccagacgtg cctggtcgag ttggtgatgg gatcgtcgcc gatggtccgg
     2161 catgtcttca cggtgacggg cgccggacgc tgtgacagct atgtcaactg cgcgacgagc
     2221 gtcccgtcgc gctggcggtc gttcgtcgac acgcgagcgc tgatgcaaag ccggccgcgt
     2281 gtcatctccg ggctggcaca gcgagcggag tggttggagt ccgcgcccga cacctcgtat
     2341 gtcgcgccgc gccggtcgta tgggtctgct gtcgcggggc ggcgctaccg atgaccaccg
     2401 acgtcgcggc gagtgcagcc cccctcgagc aactacctat cgcacgagag ctcgaggggg
     2461 gctttttcgt cgtcacgacc gactcgacga tgtggctcgt cgacactcgt cagcggcgct
     2521 tccaacgcca cggtcatgac gtcgccgtcc aagacgcgct gacgttcggc acgtggcagc
     2581 cgtacgaacg cgcgcaatgg caggcgcccg acacgttcct gatcgtcccg acgcgctcgc
     2641 gcgtcctgat ccgctgcgag aaagcgcgcg tgtgccggcc tcgtctgtga ggcgcgccgc
     2701 gatctgacga gctcagctca ctgcgatcgc gccgacgaac aggtccgacg gcagtgcgac
     2761 acggtgcgtg accttctccg atgcggggac gatcttcgcg atgacgacgt cgtgctgttg
     2821 gggatcgtcg cttgcctgca ccaacgcgaa gaccgcgtcg cgcccggcga cgaccgagac
     2881 cggaatgcgg tcggccgtcc cggttcgctc gacgagtggg atgacggtgg gcacggcgtc
     2941 gttcgcatgg agcgcgtaca ggtcgtgcgc gttgccgccc gtgaaccaca cggcgccatt
     3001 cgccgcggcc gcgctgtgca gtggagtcgc gtcccgtggt gcagagatcg tcgccggatc
     3061 atccaatgcc gtcgtggaca gggtcgtcac cgacgtcgcg gtgacgatcc aaagatgcgc
     3121 gtcgtcggcg gcgaccgcga cgggtgcatc gctcccggca acggtcgccc gccgttccac
     3181 agcgctgttc cgcagaagcg tgacgaccca ttccgatggt gtctgcgtca ctgcgtacag
     3241 gtcggtgccg gatgcggcaa ccgtcgtgtc gatcgcgccg gcgacgccga ggtcgatcga
     3301 ccccgcgacg tcgagcgtct ttgcgtcgag ttgggtcacc gttgccgggc ccgtgacgag
     3361 cgaaagcgtg tcatcggcga tcgcaatgct ctcgagct
//
LOCUS       DASUGA010000091         3083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1784679, whole genome shotgun sequence.
ACCESSION   DASUGA010000091 DASUGA010000000
VERSION     DASUGA010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1784679"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VFX21_01665"
     CDS             <1..912
                     /locus_tag="VFX21_01665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5094685.1"
                     /translation="AIASRTERMRLGYGVRLMPKPYNHPVRTAESVAVLDLLSDGRVD
                     FGTGRSATRLELEGFGIDPKDTREMWREAIEHVVDCWTNERTSFEGKHWSLPDRRVQP
                     KPLQDPHPPIWGATTSDEGHQQVGELGLGLCSFAVGVSPEDVKKKIDIYRESVEKCTT
                     AIGKFVNNRAATFTMACCAPTTEEAWATARESFEWYPKVGARQIAQVAEWMEERKQGL
                     GNYDYAADMRKHSDDGSLDLLSIEYLAETGACVLGTPDQCVEACRRYEEAGVDLLLCL
                     VNPYKIPHDKVMQTIDLFGTHVIPEFA"
     gene            909..1667
                     /locus_tag="VFX21_01670"
     CDS             909..1667
                     /locus_tag="VFX21_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013873573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX5094686.1"
                     /translation="MSGDFQIVCEIEPATTPDLKRVRHQIGVLSRVADAFLVPDNHIG
                     RATVSSVAVAHEVGDMGERAIACLNARDRNVLGLRRDLLTAAAYGVEEFLLVYGDRPS
                     AGARVDQLTVRTMIEEFRTFEAGRNFRVGVTTGLRPLPAWKQAADFCFVQIGFDVDAL
                     KRWRDTVSFDGPIYAGVIVLASPAMARKLSSDIADISVPDGALAALEGDRNAGVELAC
                     DLIDDIRATGAFAGVHLVPVGRYREIAARLESRR"
     gene            1678..2451
                     /locus_tag="VFX21_01675"
     CDS             1678..2451
                     /locus_tag="VFX21_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEX5094687.1"
                     /translation="MSVPRFAGKHEHTEFLSPTAMHAWRREHGMLPERRIDSAVILYQ
                     DQLYRAVPDLEATTKLGGQGMYRRVVTLDRTDGRVGVIGGFGIGAPVAAVVLEELIAI
                     GAQRVVSMGTAGGLQPDLAAGDVVVCTSAVRDEGVSHHYAPAEVIACPDAALTDDLER
                     AIVARGLPARRGAAWTIDTPYRETAAEARHYQAEGVQCVEMEASALFTVAAYRNVQLA
                     SAFCISDSLADAEWNPMFDHADLVTNMLGLFNAAVDTLA"
     gene            complement(2570..>3083)
                     /locus_tag="VFX21_01680"
     CDS             complement(2570..>3083)
                     /locus_tag="VFX21_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020557688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5094688.1"
                     /translation="ELKTVLWPLPSDSDLKSWQMRAEAWNHPHVMIGGSDAGAHLDRM
                     CGAPYTTSFLGDSLRGRQLVSLERCVQLMTQAPAQLFGLRDRGELREGAHADVVVFDP
                     ATVDTGDVQLIDDLPGAERRLYADAIGVQRVMVNGTTIVTDGKETGARPGTVLRSGRD
                     TYTVPIPADA"
BASE COUNT          476 a         1038 c         1096 g          473 t
ORIGIN      
        1 gcgatcgcat cgcgtaccga acggatgcgt ctcggctatg gcgtgcgctt gatgcccaag
       61 ccgtacaacc acccggtgcg caccgcggag tcggttgcgg tgctcgacct gttgagcgac
      121 ggccgcgtcg acttcggtac cggccgctcg gcgacgcgcc tcgagctcga agggttcggc
      181 atcgacccga aggacacgcg cgagatgtgg cgcgaggcga tcgagcacgt cgtcgactgc
      241 tggaccaacg agcgcacgtc gttcgagggc aagcactggt cgctgcccga ccggcgcgtg
      301 cagccgaagc cgctccagga tccgcacccg ccgatctggg gcgcgacgac gagcgacgaa
      361 gggcaccagc aggtcggtga gctcgggctc ggcttgtgct cgttcgcggt cggcgtgtcg
      421 cccgaggacg tgaagaagaa gatcgacatc taccgcgagt ccgtcgagaa gtgcaccact
      481 gcgatcggca agttcgtgaa caaccgcgcc gcgacgttca cgatggcgtg ctgcgcgccg
      541 acgaccgaag aagcgtgggc gaccgcgcgc gagtcgttcg agtggtaccc gaaggtcggt
      601 gcgcgccaga tcgcacaggt cgcggagtgg atggaagagc gcaagcaggg cctcggcaac
      661 tacgactacg cggccgacat gcgcaagcac tccgacgacg gctcgctcga cctcttgagc
      721 atcgagtacc tcgcggagac cggtgcgtgc gtgctcggga cgcccgacca gtgcgtcgaa
      781 gcgtgccggc gctacgagga ggcgggcgtc gacctgctgc tgtgcctcgt gaacccctac
      841 aagatcccgc acgacaaggt gatgcagacc atcgacctgt tcgggacgca cgtcatcccc
      901 gagttcgcgt gagcggcgac ttccagatcg tctgcgagat cgagccggcg accacgccgg
      961 acctcaagcg tgtgcgccac cagatcggtg tgctgtcgcg cgtcgccgac gcattcctcg
     1021 tgcccgacaa ccacatcggc cgggcaaccg tgtcgagcgt cgccgtcgcg cacgaggtcg
     1081 gcgacatggg cgagcgcgcg atcgcatgcc tgaacgcgcg tgaccgcaac gtgctcggcc
     1141 tgcgccgcga cctgttgacc gcggccgcgt acggcgtcga ggagttcttg ctggtgtacg
     1201 gcgaccggcc gtcggcgggc gcgcgcgtcg accagctcac cgtgcggacg atgatcgagg
     1261 agttccgcac cttcgaagcc gggcgcaact tccgcgtcgg tgtcacgacc ggcctgcggc
     1321 cgttgccggc atggaagcag gccgccgact tctgcttcgt gcagatcgga ttcgacgtcg
     1381 acgcgctgaa gcgctggcgc gacacggtgt cgttcgacgg tccgatctat gcgggcgtga
     1441 tcgtgctcgc gagcccggcg atggcccgca agctctcgag cgacatcgcc gacatctccg
     1501 ttcccgacgg ggcacttgcg gcattggaag gtgaccgcaa cgcgggcgtc gagctcgcgt
     1561 gcgacttgat cgacgacatc cgcgccaccg gcgcgttcgc gggcgtgcac ctcgtccccg
     1621 tcggccgcta ccgagaaatc gcggcgcgcc tggagtcccg tcggtaacaa tcggagcgtg
     1681 agcgttccga ggttcgcggg caagcacgag cacaccgagt tcctgagccc gacggccatg
     1741 catgcgtggc ggcgcgagca cggcatgctg ccggaacgca ggatcgacag tgccgtcatc
     1801 ctgtaccagg accagctgta ccgcgcggtg ccggatctcg aagcgacgac gaagctcggc
     1861 ggtcaaggca tgtaccgccg cgtcgtcacg ctcgaccgga ctgatggtcg cgtcggcgtg
     1921 atcggcggct tcggcatcgg cgcgcccgtg gccgcggtcg tgctcgagga gctgatcgcg
     1981 atcggtgcgc agcgagtcgt gtcgatgggc acggcgggcg gactgcaacc tgatctcgcc
     2041 gcgggcgacg tcgttgtgtg cacgagtgcc gtgcgcgatg aaggggtctc gcaccactac
     2101 gcgcccgcgg aggtcatcgc gtgccccgac gccgcgctca ccgacgatct cgagcgtgcg
     2161 atcgtggccc gcggtctgcc ggcccggcgc ggcgccgcat ggacgatcga caccccgtac
     2221 cgcgagaccg cggccgaagc ccgccactac caggccgagg gtgtccagtg cgtcgagatg
     2281 gaagcatccg cgctgttcac agtggcggcg taccgcaacg tgcagctcgc gtccgcgttc
     2341 tgcatcagcg actcgctcgc cgacgcggag tggaacccca tgttcgacca cgccgacctg
     2401 gtcacgaaca tgctcggttt gttcaacgcc gcggtcgaca ccctggcgta gcgcgtacct
     2461 gcgcctttga accacccggc cggtggttgg gggacgccgg tacgcgcgcc cgatcgaacc
     2521 tgcgcctttg aaccaccggg gcggtggtcc agggacgccg gtacgcgcgc tatgcgtcgg
     2581 ccgggatcgg gacggtgtag gtgtcgcggc cgcttctcag gacggtgccg gggcgggcgc
     2641 cggtctcctt gccgtcggtg acgatcgtcg tgccgttgac catcacgcgc tggacgccga
     2701 tcgcgtcggc gtagaggcgg cgttcggcgc cggggaggtc gtcgatcagc tgcacgtcgc
     2761 cggtgtcgac cgtggccggg tcgaacacga cgacgtccgc gtgcgcgccc tcgcgcagct
     2821 cgccgcggtc gcgcaggccg aagagctgtg cgggtgcttg cgtcatcagt tgtacgcagc
     2881 gttcgagcga gacgagctgg cggccgcgca gcgagtcgcc gaggaacgac gtcgtgtacg
     2941 gcgcgccgca catgcggtcg aggtgcgcgc cggcatcgga gccgccgatc atcacgtgcg
     3001 ggtggttcca cgcctcggcc cgcatctgcc acgacttcaa gtcgctgtcg ctcggcagcg
     3061 gccacagcac cgtcttgagc tcg
//
LOCUS       DASUGA010000092         6272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_115822, whole genome shotgun sequence.
ACCESSION   DASUGA010000092 DASUGA010000000
VERSION     DASUGA010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6272
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115822"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VFX21_01685"
     CDS             <1..753
                     /locus_tag="VFX21_01685"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX5094689.1"
                     /translation="AREKAGIVKADSLLVLGERDDDLAQIFIDRAPRAVVRRDVDFGV
                     RDNALAVGGRLLELYTPRAAYADVVLPLHGAHQADNAAIALMAAEGFVNGTLDPEVVA
                     DAFSRVRSPGRLEVVRRQPLVLLDGAHNVAGAEALRASLDEAFATGPRTLVVGLLREK
                     EPHEMLAALGLDDVSLLIVCRAPSPRALEPEAVAKAAIDLGFAGDRIELAETVPEAVT
                     NALLETPEDGQIVITGSLYVVGAARGLLVRGS"
     gene            765..1862
                     /locus_tag="VFX21_01690"
     CDS             765..1862
                     /locus_tag="VFX21_01690"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5094690.1"
                     /translation="MTDLRVGVIGTGVGLAHLFAIGAVDRMEIGAVCDVDETRAEKSA
                     ADFGAPAFGDPAVLFASGSIDAVVIATPGATHAALTREALDAGLHVYCEKPITPTSDE
                     GYALARYARDRDKVLVVGFQHRYNPRYVAVADAVRELGPLRRFELRATNWFRSQHYFD
                     VSPWRATWRMAGGGVLMNQAIHQLDALIAIAGMPTRVSARVSVRAHRTETEDEALAWL
                     EWHDGATGSLVASLNDATGEERFQCTCERGAVVLSADGVVRRIAHDATREIVDAATES
                     EPPLVRHWQEVDTGKYSEFDMFKNMHRNFAAAILDGAKPRVDGEEGTQAVELANAIYM
                     SSVAGEPVDLPLDRGAYPPVFEELSLGDRSI"
     gene            1898..2317
                     /gene="ndk"
                     /locus_tag="VFX21_01695"
     CDS             1898..2317
                     /gene="ndk"
                     /locus_tag="VFX21_01695"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018500225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX5094691.1"
                     /translation="MSNENRTLVLCKPDCVERGLVGEVVARFERKGLKLVALELRQLD
                     EATAKQHYAEHDGKPFFGDLVSFITRSPLVAMVVEGTDAWKVVRTMMGTTNPREAAPG
                     TIRGDYAIELTENLVHGSDGPESAAREIALFFPTLGA"
     gene            2421..3458
                     /locus_tag="VFX21_01700"
     CDS             2421..3458
                     /locus_tag="VFX21_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX5094692.1"
                     /translation="MSDPWYSSVLGRDMAVDLGTANTLVYVRGRGVVLDEPSVVAVNA
                     RDGRPLAVGIEAKKMIGRTPGHIQAIRPLKDGVIADFDICEKMLRYFIQKVHPRKWAK
                     PRMVICVPSGITGVEQRAVQEAAQQAGARKEAYIIEEPMAAAIGAGLPVHEPAGNMIV
                     DIGGGTTEVAVISLGGIVASESIRIAGDELDEAIVNFVKKEYSLALGERTSEEIKIAL
                     GSAFPLQEELYAEVRGRDLVTGLPKTIVVSTEEIRDAIEEPVAAIVDAVKVTLDKTPP
                     ELAADIMSKGIVVTGGGAMLHGLRERLSTETGMPIVIADNPLFSVVHGSGQCLETFDV
                     LKHVLISSAQQ"
     gene            3510..4397
                     /gene="mreC"
                     /locus_tag="VFX21_01705"
     CDS             3510..4397
                     /gene="mreC"
                     /locus_tag="VFX21_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX5094693.1"
                     /translation="MLLIIVLTALTLITLDARGGRTGPLGFLGRAAHTVVSPIQGAVD
                     DVASPVSDWWQGVTNSGDIKQENQTLKEDLAKAQGEARANAKAAAENEELRKQLGLTD
                     SINGESVLAEIRGGEVGNFDPTFTINKGTRDGVAKDMPVISPAGLVGQVIEVWSNGAK
                     VRTLTDPKFAIAVVTPPQGASRPVSDTANGTGGPTLEASFGAGTKLKVGDKIYSSALS
                     SSFPPNIAVGTVSKVTESPGNTSVKVEITPFVNLDELQYLTVLKWHPGAAPPVTLPTT
                     TSTTTTTIANPFGVTSTVP"
     gene            4397..4906
                     /gene="mreD"
                     /locus_tag="VFX21_01710"
     CDS             4397..4906
                     /gene="mreD"
                     /locus_tag="VFX21_01710"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX5094694.1"
                     /translation="MRVVRLALLIVCALIVQVAVFPHLRLFGVVPDLGLLIAVAVGYE
                     YGAEAGALTGFFAGLGYDLFLETPLGLCALSYAIVGYFMGIIESGLFRSPRWLPSLLG
                     AIGGLAAGLLFIAIGVLAGEEIVKGQHAVITISLAALYDALLAPLVFFLVRRVLGREQ
                     RVRDAWSMK"
     gene            4984..>6272
                     /locus_tag="VFX21_01715"
     CDS             4984..>6272
                     /locus_tag="VFX21_01715"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX5094695.1"
                     /translation="MSDNSRVRVSLVGVVIVALFGSLLARLWFLQVGPEQSTLGALSV
                     SLTTRQVRNDMPRGRILDRNGVVLAQDRAAWAVTVSRDLDEEAREKILGQLSELLGIP
                     QKTLQERFDSVQQSVLKPAVVALDVPLDKRIVLKERQDDYPGVNLEELTVRTYPQDRL
                     AAQTIGYTGEIDAEQLKKLKARGYVAGDSIGRAGIEAAYEKQLRGTPEQQTVQVDPTG
                     KQVGEPIKTIAGKPGDDVVLTIDSRVQRVAEQALAQGIASARTRQNKDVKNKYETFKA
                     TEGAVVVLDVRDGSVAAMASYPDYSLKLWEGGITTQQFQILNSDLAGHPLVNRATEGG
                     YAPGSTFKLVTGLATPRYGMRSIGEYYDDNGFVYIGADRQKRSNDNGVVNGPVNLTQA
                     LMVSSDTYFYTAGEQFYFALQRGETDRGLGLQTQAREL"
BASE COUNT         1094 a         2047 c         2118 g         1013 t
ORIGIN      
        1 gcacgcgaga aggcgggcat cgtgaaagcc gactcgctcc tcgtgctcgg cgagcgcgac
       61 gacgacctcg cgcagatctt cattgatcgc gcgccccggg ccgtcgtacg ccgcgatgtc
      121 gatttcggtg tgcgcgacaa cgcgcttgcg gtcggcggac ggttgctcga gctctacacg
      181 ccgcgggccg cctacgccga cgtcgtgttg ccgctgcacg gcgcgcacca agccgacaac
      241 gcggcgatcg cgctcatggc cgcggaaggt ttcgtcaacg gcacgctcga tccggaggtc
      301 gtcgccgacg ccttctcgcg tgtgcggtcg cccggccggc tcgaggtggt gcgccgccaa
      361 ccgctcgtac tgctcgacgg ggcgcacaac gtcgccggtg ccgaagcgct gcgggcgtcg
      421 ctggacgaag cgttcgccac cgggccgcgg accctcgtcg tcgggctgtt gcgcgagaag
      481 gagccccacg agatgctcgc cgcgctcggg ctcgacgacg tctcgttgct catcgtctgc
      541 cgcgcgccga gcccgcgtgc gctcgaaccg gaagcagtcg ccaaggctgc catcgacctc
      601 ggtttcgcgg gggatcggat cgagttggcg gaaaccgtcc ccgaggcggt caccaacgcg
      661 ctcctcgaaa cgcccgagga cggtcagatc gtgatcaccg gctccctgta cgtcgtcggc
      721 gccgcccggg gactgctcgt ccgcgggtcg tagcatcccg caccatgaca gatctgcgcg
      781 tcggtgtgat cggcacgggc gtcgggctcg cacatctctt cgcgatcggc gcggtcgacc
      841 ggatggagat cggcgcggta tgcgacgtcg acgagacacg cgccgagaag agcgccgccg
      901 acttcggcgc accggccttc ggcgatccgg cggtgttgtt tgcgagcggt tcgatcgacg
      961 cggtcgtgat cgcgacgccg ggtgccaccc acgccgcgct cactcgtgag gcgctcgacg
     1021 cgggattgca cgtctattgc gagaagccga tcacgcccac gagcgacgag gggtatgcgc
     1081 tcgcgcgtta cgcgcgcgat cgggacaagg tgctcgtcgt cggcttccaa caccgctaca
     1141 acccgcgcta cgtcgcggtg gccgacgcgg tgcgcgagct cggcccgttg cgccgcttcg
     1201 agctgcgggc aacgaactgg ttccgctccc agcactattt cgatgtgagc ccgtggcgcg
     1261 cgacgtggcg catggcgggc ggcggcgtgc tgatgaacca ggccatccat cagctcgacg
     1321 cgctgatcgc gatcgcggga atgccgaccc gagtcagcgc gcgggtgagt gttcgcgccc
     1381 atcgcaccga gaccgaggac gaagcgctcg cgtggctgga gtggcacgac ggtgcaaccg
     1441 gttcgctggt cgcgtcgctc aacgacgcga cgggtgaaga acgatttcag tgcacgtgtg
     1501 aacgcggcgc ggtcgtgctc tccgccgacg gcgtcgtacg tcgtatcgcg cacgacgcga
     1561 cacgcgagat cgtcgatgcg gcgaccgaga gtgagccgcc gctggtacgc cactggcaag
     1621 aggtcgacac cggcaagtac tccgagttcg acatgttcaa gaacatgcac cgcaacttcg
     1681 cagccgcgat cctcgacggt gcgaagccgc gcgtcgacgg cgaggagggc acgcaggccg
     1741 tcgagctcgc gaacgcgatc tacatgtcgt cggtcgcggg cgaaccggtc gacctgccgc
     1801 tcgatcgcgg cgcctacccg ccggtgttcg aggaactgag cctcggcgac cggtcgatct
     1861 gagccctgac caggggctga agtagcctca ccggcttatg agtaatgaga accggacgtt
     1921 ggtcttgtgc aagcccgact gcgtcgagcg cgggctcgtc ggtgaagtcg tcgcccgttt
     1981 cgagcgcaag ggactgaagc tcgtcgcgct ggagctgcgc cagctcgacg aagcaacggc
     2041 gaagcagcac tacgccgagc acgacggcaa gccgttcttc ggcgacctcg tctcgttcat
     2101 cacccgcagc ccgctcgtcg cgatggtcgt cgaaggaacc gacgcgtgga aggtcgtgcg
     2161 cacgatgatg ggcacgacga acccgcgtga ggctgcgccc ggcaccatcc gaggggacta
     2221 cgcgatcgag ctcaccgaga acttggtgca cggcagcgac ggtccggagt cggccgcgcg
     2281 cgagatcgcc ctgttcttcc caacgctcgg cgcctgagcc gccgaatcga ccacggagcg
     2341 cagtgagcga acctgggtgg cgccgccccg cctccgctcg tagccttggt ggaccgcgcc
     2401 ggcagcccgg gaggactcgg gtgagcgatc cctggtattc ctccgttctc ggccgcgaca
     2461 tggcggtcga cctcggtaca gccaacacgc tcgtctacgt gcgcggccgc ggtgtcgtcc
     2521 tcgacgaacc gtcggtcgtt gccgtcaacg cgcgcgacgg gcgtccgctc gcggtcggca
     2581 tcgaagcgaa gaagatgatc ggccgcacgc ccggacacat ccaggcgatc cggccgttga
     2641 aggacggcgt catcgccgac ttcgacatct gcgagaagat gcttcggtac ttcattcaga
     2701 aggtgcatcc gcgcaagtgg gcgaaaccgc ggatggttat ctgcgtgccg tcgggcatca
     2761 cgggcgtcga gcagcgcgcg gtgcaggaag ccgcccagca agccggcgcg cgcaaagagg
     2821 cgtacatcat cgaagagccg atggcggccg cgatcggcgc cgggctgccg gtgcacgagc
     2881 cggccggcaa catgatcgtc gacatcggcg gaggcacgac ggaagtcgcg gtcatctcgc
     2941 ttggcggcat tgttgccagt gagtcgattc gcatcgccgg cgacgagctc gacgaagcca
     3001 tcgtcaactt cgtaaagaag gaatactccc tggcgctcgg tgagcggacg tcggaagaga
     3061 tcaagatcgc gctcggcagt gcgttcccgt tgcaagagga gctctacgcc gaagtgcgcg
     3121 gccgcgacct cgtgacgggc ttgccgaaga cgatcgtcgt gtcgacggaa gaaatccgcg
     3181 acgccatcga ggaaccggtt gccgccatcg tcgacgcggt gaaagtgaca ctcgacaaga
     3241 ccccgcccga actcgcggcc gacatcatgt cgaagggcat cgtcgtgacc ggaggcggcg
     3301 caatgttgca cggcctgcgc gaacgactct cgaccgaaac cgggatgccg atcgtcatcg
     3361 ccgacaaccc gttgttctcg gtggtgcacg gctcggggca atgcctcgag actttcgacg
     3421 tcctgaagca cgtcctgatc agctccgccc agcaataggc ggaactccgg aggatggtcg
     3481 cagccagccg cgcgcgccga cgccgatcgg tgctcctgat catcgtcctg accgcgttga
     3541 ccctgatcac actggatgcc cggggcggcc ggaccggccc gctcgggttc ctgggccggg
     3601 ccgcccacac cgttgtttcg cccatccagg gagcggtcga cgacgtcgcc agccccgtct
     3661 ccgactggtg gcaaggggtc acgaactcgg gcgacatcaa gcaggagaac cagaccctca
     3721 aggaagactt ggccaaggca cagggcgagg cgcgcgcgaa cgcgaaggcc gcggccgaga
     3781 acgaagagct gcgcaagcaa ctcggactca ccgacagcat caacggcgaa tcggtgctgg
     3841 cagagattcg cggcggcgaa gtcggcaact tcgatcccac gttcaccatc aacaagggca
     3901 cgcgcgacgg tgtcgcgaaa gacatgcccg tcatctcgcc ggccgggctc gtcggtcaag
     3961 tcatcgaagt ctggagcaac ggcgcgaagg tgcgcacgct caccgatccg aagttcgcga
     4021 tcgcggtggt gacgccgccg caaggtgcga gtcggccggt ctccgacact gcgaacggca
     4081 ccgggggtcc gacgctcgaa gcgagcttcg gcgcgggaac gaagctgaag gtcggcgaca
     4141 agatctatag ctccgcgctg tcgtcgtcgt tcccgccgaa catcgcggtc ggcacggtgt
     4201 caaaggtgac cgagagcccc ggcaacacga gcgtgaaagt agaaatcacg ccgttcgtca
     4261 atctcgacga actgcagtac ctcacggtgc tgaaatggca tccgggcgcg gcaccgccgg
     4321 tgacattgcc gacaaccacg tcgacgacaa ccaccaccat cgccaacccc ttcggcgtga
     4381 catcgacggt gccgtgatgc gtgtcgtccg gcttgccttg ctcatcgtct gtgcgctcat
     4441 cgtgcaggtc gcggtttttc cccacttgcg actgttcggt gtcgtgcccg acctcgggct
     4501 gttgatcgcg gtcgcggtcg gttacgagta cggcgccgag gccggcgcgc tgaccgggtt
     4561 cttcgcgggg ctcggttacg acctcttcct ggaaacaccg ctcggcttgt gcgcgctctc
     4621 gtatgcgatc gtcggctact tcatgggcat catcgaatcg ggattgttcc ggtcgccgcg
     4681 ctggctcccg tcgttgctcg gtgccatcgg cggtctcgcg gccggtttgc tgttcatcgc
     4741 gatcggtgtg ctcgccggtg aggaaattgt gaaaggtcag cacgccgtga tcacgatctc
     4801 gctcgccgcg ttgtacgacg cgttgctggc accgcttgtg ttcttcctgg tgaggcgggt
     4861 gctcggccga gaacaacgag ttcgtgacgc ctggtcaatg aaataacgca gagccgaccg
     4921 ggactcgacc ggcgacggcc actagcgtga cacccggctc gcggagggcc cttttctttc
     4981 tgcatgagtg acaacagccg cgttcgcgtc agtctggtcg gcgtcgtcat cgtcgccttg
     5041 ttcggttctc tcctcgcgcg cctgtggttt ctccaagtcg ggccggagca gagcacgctg
     5101 ggcgcgttga gcgtctcctt gacgacgcgt caggtccgca acgacatgcc gcgcggccgc
     5161 atcctcgacc gcaatggtgt cgtactcgcg caggaccgag ccgcgtgggc ggtcaccgtg
     5221 tcgcgagacc tcgacgagga agcgcgcgag aagattctcg gccagctctc cgaactgctc
     5281 ggcattccgc agaagacgct ccaggagcgg ttcgactcgg tgcagcagtc ggtgctgaag
     5341 ccggcggtgg tcgcgctcga cgtgccgctc gacaagcgga tcgtcctcaa ggaacgccag
     5401 gacgactatc ccggcgtcaa tctcgaagaa ctcacagttc ggacgtatcc gcaggatcgc
     5461 ctcgccgcgc agacgattgg ctacacgggc gagatcgatg ccgagcagct caagaagctg
     5521 aaggcgcgcg gctacgtcgc gggcgactcg atcggccggg ccggtatcga ggcggcgtac
     5581 gagaagcagt tgcgcggcac gccggaacag cagaccgtgc aggtcgaccc gaccggaaag
     5641 caggtcggcg aaccgatcaa gacgattgcc ggcaagcccg gtgacgacgt cgtgctgacg
     5701 atcgattcgc gcgtccagcg cgtcgcggag caagcgctcg cgcaaggcat cgcgtcggcg
     5761 cgcacccgtc agaacaagga cgtcaagaac aagtacgaga cgttcaaggc aaccgaaggc
     5821 gcggtcgtcg ttctcgatgt tcgcgacggt tcggtcgcgg cgatggcgag ctatcccgac
     5881 tattccctga agttgtggga gggcgggatc accacccagc agttccagat cctgaacagc
     5941 gatctggcgg gccatccgct ggtcaaccgg gcaaccgagg gcgggtacgc gcccggatcc
     6001 acgttcaagc tcgtgaccgg cctcgcgaca ccgcgctacg gcatgcggag catcggggag
     6061 tactacgacg acaacggctt cgtgtacatc ggcgccgacc gccagaagcg gtcgaatgac
     6121 aacggcgtcg tcaacggacc cgtcaatctg acgcaagcgt tgatggtttc gagcgacacg
     6181 tacttctaca cggcggggga gcagttctat ttcgcgcttc aacgcggtga gacggatcgt
     6241 gggttgggcc tccagaccca agcgcgcgaa ct
//
LOCUS       DASUGA010000093         3364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_672568, whole genome shotgun sequence.
ACCESSION   DASUGA010000093 DASUGA010000000
VERSION     DASUGA010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672568"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VFX21_01720"
     CDS             complement(<1..433)
                     /locus_tag="VFX21_01720"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEX5094696.1"
                     /translation="MRDALALLTTVGRRGGALSARALGWFPLVGAALGAVLGGWWWAA
                     DKWWPAGLAAVLVVVADLALTGMLHMDGVADAADGLLPHASTERRLEIMSTPDVGAFG
                     AVAVGAMLLLRVFAFASMAVSVPLVVALWCSSRALVASVPAV"
     gene            complement(433..924)
                     /locus_tag="VFX21_01725"
     CDS             complement(433..924)
                     /locus_tag="VFX21_01725"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEX5094697.1"
                     /translation="MITLVLGGNRSGKSEVAERLAAATGEPISYVATADASAEGFGPR
                     IDAHRARRPATWVTVEQPVDLPGTLMRLEGTVLVDSLGMWVATAPGFGADVEGLACAL
                     TRRLGSTVLVSDEVGLGVHPPTEIGVQFADALGACNRRIAEVSDRVVLVVAGRTLELP
                     REI"
     gene            complement(921..1514)
                     /locus_tag="VFX21_01730"
     CDS             complement(921..1514)
                     /locus_tag="VFX21_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019855429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HEX5094698.1"
                     /translation="MSGFDESVLVWRFDAPRLAIASAPVGGGIGLRSWVLNAQVPRDY
                     SRTDLDAHVASIAAAEGCTGDGVGMLTAVPMSFARYASDRGVEVCATVGVTRPTWAAD
                     ATDAISAWTPGTINIVAFVPARLSEAALVNAVMTVTEAKVQALSEGGVPGTGTASDAA
                     CVVCPASGPVQAFGGPRSGLGAPLARAVCEAVRGGIS"
     gene            complement(1510..2076)
                     /gene="cobO"
                     /locus_tag="VFX21_01735"
     CDS             complement(1510..2076)
                     /gene="cobO"
                     /locus_tag="VFX21_01735"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX5094699.1"
                     /translation="MTAQPPTDTPDKSGMRIAKSLIVVNTGDGKGKSSAAFGVVMRAI
                     ARGWKVAVVQFLKSGDWHVGEEDVSRQLGVDFWAEGEGFTWESEDLTRDQAVARAGWE
                     HAKSIINSGTYRLVVLDEITYPINWGWIDGDDVVTTFRNRPETVNIVATGRDAPAALV
                     EVADTVTEMRKVKHAFDTGIAAKKGIDY"
     gene            2125..2394
                     /locus_tag="VFX21_01740"
     CDS             2125..2394
                     /locus_tag="VFX21_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094700.1"
                     /translation="MRRGIAVGIVAATVLLGACASDAYDPDSVTQDLRDAGLSQRDAD
                     CVVAEMRSTFGSDRLSDRGEPTNEERAKFDALLRECTSADEAPTS"
     gene            complement(2366..2896)
                     /gene="pyrE"
                     /locus_tag="VFX21_01745"
     CDS             complement(2366..2896)
                     /gene="pyrE"
                     /locus_tag="VFX21_01745"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX5094701.1"
                     /translation="MAEPDREKLAQRIYDTAHLTGTFTLRSGVTSTEYFDKYRFEADP
                     GLLADIARAMARLVPAGWDILAGLELGGVPLATMLSQVTGLPARFVRKEAKTYGTCQL
                     AEGGELDGRRLCIVEDVVTSGGAILDAAVELRARGAVLGPVVCVIDRESGGAEKLADA
                     GLELRALFRMSELRPR"
BASE COUNT          501 a         1227 c         1152 g          484 t
ORIGIN      
        1 cgaccgcggg gaccgacgcg acgagcgcgc gcgacgagca ccacaacgcg acgaccagtg
       61 gcaccgagac ggccatcgac gcgaacgcga acacccgcaa caacaacatc gcaccgaccg
      121 ccacggcacc gaacgcgccc acgtccggtg tgctcatgat ctcgagccgc cgttcggtcg
      181 acgcgtgcgg caacaagccg tcggccgcgt cggcgacacc gtccatgtgc aacatgcccg
      241 tcaacgcgag gtcggccacg acgacgagta cggccgccag ccccgccggc caccacttgt
      301 cggcggccca ccaccatccg cccaggaccg cgcccagcgc agcgccgacg agcgggaacc
      361 aacccaacgc gcgcgccgac agcgcgccgc cgcggcgacc gaccgtggtg aggagcgcga
      421 gcgcgtcgcg catcagatct cccgcggcag ttcgagggtg cggcccgcga ccaccagcac
      481 aacacggtcc gacacttccg cgatgcggcg attgcacgcg ccgagcgcgt cggcgaactg
      541 cacaccgatc tcggtgggcg ggtgcacgcc cagccccact tcgtcggaga cgagcaccgt
      601 gctcccgagc cgccgggtca gcgcgcacgc cagcccttcc acgtcggcgc cgaagccggg
      661 cgcggtcgcg acccacatcc cgagggagtc gacgagcacg gtcccctcga ggcgcatgag
      721 cgtgccgggc aggtcgacgg gttgctccac cgtgacccag gtcgcggggc gccgcgctcg
      781 gtgcgcgtcg atgcgcgggc cgaaaccctc tgcgctcgcg tcggctgtcg cgacgtacga
      841 gatcggctcg cccgttgccg ctgccagccg ctccgcgact tccgacttcc ccgagcggtt
      901 gccgccgagc acgagcgtga tcacgagatg ccgccgcgca ctgcctcgca gaccgcgcgc
      961 gccaacggcg cgcccagccc cgagcgcggc ccgccgaacg cctgtaccgg accggacgcg
     1021 ggacacacga cgcaggcggc gtcgctcgcg gtgccggtcc cgggcacgcc gccctccgaa
     1081 agggcttgca ccttcgcttc cgtcaccgtc atcaccgcgt tgacgagcgc ggcctccgag
     1141 aggcgcgcgg gcacgaatgc gacgatgttg atcgtgccgg gcgtccacgc gctgatcgca
     1201 tcggtagcgt cggcggccca cgtcgggcgg gtcacaccga cggtcgcgca cacttcgaca
     1261 ccgcggtcgg aggcgtaccg tgcgaacgac atgggcaccg cagtcagcat tccgacgccg
     1321 tcgcccgtgc agccctcggc ggccgcgatc gatgcgacgt gcgcgtcgag gtcggtacgc
     1381 gagtagtcgc gcggcacctg cgcgttgagc acccacgaac gcaacccgat gccaccgccc
     1441 acgggagccg acgcgatcgc gagccggggc gcgtcgaagc gccagacgag gacggattcg
     1501 tcgaacccgc taatagtcga tgcccttctt ggccgcgatc ccggtgtcga acgcgtgctt
     1561 caccttgcgc atctcggtca ccgtgtcggc gacctcgacg agcgccgcgg gcgcgtcgcg
     1621 tcccgttgcg acgatgttca cggtctccgg acggttgcgg aacgtcgtga cgacgtcgtc
     1681 gccgtcgatc cagccccagt tgatcgggta cgtgatctcg tcgagcacga cgagccggta
     1741 cgtgccgctg ttgatgatcg acttcgcgtg ttcccagccc gcgcgcgcga ccgcctggtc
     1801 gcgcgtcagg tcttcggatt cccaggtgaa gccttcgccc tcggcccaga agtcgacgcc
     1861 gagttggcgc gacacgtctt cttcgccgac gtgccagtcg cccgacttca ggaactgcac
     1921 gaccgcgacc ttccaaccgc gagcgatggc gcgcatcacg acgccgaacg ctgcactcga
     1981 cttgcccttg ccgtcaccgg tgttgacgac gatcagcgac ttcgcgatcc gcatcccgct
     2041 cttgtcggga gtgtcggtcg gcggctgcgc ggtcatgggc cctcgatgat aaaggcgaac
     2101 cccggcggac gtagatttcc ctgcgtgcgc cgtggaattg ccgtcgggat cgtcgccgcg
     2161 accgtgctgc tgggcgcgtg cgcgagtgac gcgtacgacc ctgattccgt cacccaggac
     2221 ctgcgcgacg ccgggctgag tcagcgggac gccgactgcg tggtcgccga gatgcgcagc
     2281 acgttcggct ccgaccggct ctcggatcgg ggcgagccga ccaatgaaga gcgcgccaag
     2341 ttcgacgcgc tgcttcgaga gtgcactagc gcggacgaag ctccgacatc ctgaacagcg
     2401 cgcgcagctc caagcccgcg tcggccagct tctcggcacc acccgactcg cggtcgatga
     2461 cgcacacgac gggaccgagc actgcgccac gggcgcgcag ctcgactgcg gcgtcgagga
     2521 tcgcgccgcc cgacgtcacg acgtcctcga cgatgcacaa ccgccgcccg tcgagctcgc
     2581 cgccctcggc gagctggcag gtgccgtagg tcttcgcttc cttgcggacg aagcgggccg
     2641 gcagcccggt gacttgcgag agcattgtcg ccagggggac gccgcccagt tcgaggccgg
     2701 ccaggatgtc ccagcccgcg ggcacgagcc gcgccatggc gcgcgcgatg tcggccagca
     2761 gcccgggatc ggcctcgaag cggtacttgt cgaagtactc ggtgctcgtc acgcccgacc
     2821 gcagcgtgaa cgtcccggtg aggtgcgcgg tgtcgtagat ccgctgcgcc agcttctcgc
     2881 ggtcgggctc ggccatggtt cggacggtac cgcgttgacc gccgtgcgcg cggggcgtta
     2941 ccgtcctgac ctgcggaggc gggggagaca ccgcgccggc ctcgatgctg gcccggggat
     3001 gacgggaccg gggggacccg tcatcccgcc tctgtgtttc tttgtcgctt tcggctcgtt
     3061 cgcgtgggcg acttcggtgc tttgccgagc cgaaagactc cgcgagtggc cgagacggtt
     3121 gtgggggcgc tgcgcgccgt gggaacactg aggccaccgc ctctttgact tagcgcgccg
     3181 gccctcgagg ggcgctgcgc gccgcgggag ggccgaacat cgcggcactg tcgctctcgc
     3241 gccttacttt cgagcggcgg cagttcgcat ccttgccgcg aggcggtgga ggttctcgat
     3301 ctcgtcgggg agcatgttct tgtcgagtgc cttgagctcg gcggcggtcg cgatccgttc
     3361 acgg
//
LOCUS       DASUGA010000094         2714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_257170, whole genome shotgun sequence.
ACCESSION   DASUGA010000094 DASUGA010000000
VERSION     DASUGA010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257170"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VFX21_01750"
     CDS             complement(<1..351)
                     /locus_tag="VFX21_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX5094702.1"
                     /translation="MAKEKFERTKPHLNIGTIGHIDHGKTTLTAAITKVLADKNSKNT
                     FTAFDQIDKAPEEKARGITISIAHVEYETDNRHYAHVDCPGHADYIKNMITGAAQMDG
                     AILVVAATDGPMPQT"
     gene            complement(397..2481)
                     /gene="fusA"
                     /locus_tag="VFX21_01755"
     CDS             complement(397..2481)
                     /gene="fusA"
                     /locus_tag="VFX21_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX5094703.1"
                     /translation="MAREFPLEKTRNIGIMAHIDAGKTTTTERILYYTGRTYKIGEVH
                     EGAAVMDHMVQEQERGITITSAATTAQWKDCQVNIIDTPGHVDFTVEVERSLRVLDGA
                     VAVFDAVAGVEPQTETVWRQANTYSVPRICFVNKMDRVGADFERCVQMIRERLSAVPV
                     VLQLPIGAEGTFRGVIDLITMKALVWPLDDDSEGANFEVTDIPADLLEHAQEAHGRLL
                     EVIAHVDDDLLEKYLEEHEISPEEIQRAVRKGTLAFEFVPVICGSAFKNKGVQPMLDA
                     VVDYLPSPLDIPPTEGVDMKGEPTERKADENEPFSALAFKIVADPYGKLTYFRVYSGK
                     LEKGSEIYNSTKDRKERIGRILRMHSNQREDIDVAYAGDIVAGLGFKQTTTGDTLTVR
                     DQPIILEQMEFPEPVINVAIEPKTKADQDKLGKALGALSDEDPTFQVHTDEETGQTII
                     GGMGELHLEVLVDRMLREFSVEAHVGKPQVAYRETITAPVEKVEVRYVRQTGGRGQYA
                     HVVIGLEPTGPGGGFEFIDEIKGGVIPREYIPAVDAGIQEAMQGGVLAGYPMVDLRAK
                     LTFGSYHDVDSSEMAFKIAGSMALKEAAKKSKPVLLEPVMAVEVVTPEDYMGDVIGDL
                     NARRGQVEGMEQRAGAQAIRAKVPLAQMFGYVGDLRSRTQGRANYTMQFDSYQQVPES
                     IAQEIIARVRGE"
BASE COUNT          392 a          865 c          899 g          558 t
ORIGIN      
        1 cgtctgcggc atcggaccgt cggtggccgc gaccacgagg atcgcgccgt ccatctgcgc
       61 ggcacccgtg atcatgttct tgatgtagtc ggcgtggccg gggcagtcga cgtgcgcgta
      121 atggcggttg tcggtctcgt attcgacgtg cgcgatcgag atcgtgatac cgcgcgcctt
      181 ctcttccggc gccttgtcga tctggtcgaa cgcggtgaac gtgttcttgg agttcttgtc
      241 cgcgaggacc tttgtgatcg ccgccgtcag cgtcgtcttg ccatggtcga tgtgaccgat
      301 ggtcccgatg ttcaagtgcg gcttcgtgcg ctcgaacttc tctttggcca tgcgagtgcc
      361 tttgctcctt gaaagatttc gaccgaggga ctgggactac tcgccgcgta cccgggcgat
      421 gatttcttgc gcgatcgact cgggaacttg ctggtaggag tcgaactgca tcgtgtagtt
      481 cgcgcgccct tgggtgcgag accgaagatc gccaacatag ccgaacatct gcgccaacgg
      541 cactttcgcc cggatcgcct gggcgcccgc gcgctgctcc atgccctcga cctgaccccg
      601 gcgggcgttg aggtcgccga tcacgtcgcc catgtaatcc tcgggcgtga cgacctcgac
      661 ggccatgacc ggctcgagga ggacgggctt cgacttcttc gccgcctcct tgagtgccat
      721 cgagccggcg atcttgaacg ccatttcgga cgagtcgacg tcgtggtacg agccgaacgt
      781 gagcttggcc cgcaggtcga ccatcgggta gccggcgagc acgccgccct gcatggcctc
      841 ctggatgccg gcgtccaccg cggggatgta ctcgcgcggg atgacaccgc ccttgatctc
      901 gtcgatgaac tcgaagccgc cacccgggcc ggtgggctcg aggccgatga cgacgtgcgc
      961 gtactgaccg cgaccaccgg tctggcggac gtaccgcact tcgaccttct cgaccggcgc
     1021 ggtgatggtc tcgcggtacg cgacctgcgg cttgccgacg tgggcctcga cgctgaactc
     1081 gcgcagcatg cggtcgacga gcacttcgag atgcagctcg cccatgccgc cgatgatcgt
     1141 ctggcccgtc tcttcgtcgg tatgaacctg gaacgtcggg tcctcgtcgc tgagcgcgcc
     1201 gagggccttc ccgagcttgt cctggtcggc cttcgtcttc ggctcgatcg cgacgttgat
     1261 gaccggctcc gggaactcca tctgctcgag gatgatcggc tggtcgcgaa cggtgagcgt
     1321 gtcaccggtc gtcgtctgct tgaagccgag acccgcgacg atgtcgccgg cgtacgcgac
     1381 gtcgatgtct tcgcgctggt tggagtgcat ccgcaagatg cgaccgatgc gctccttgcg
     1441 gtccttcgtc gagttgtaga tctccgagcc cttctcgagc ttgcccgagt agacgcggaa
     1501 gtaggtgagc ttgccgtacg ggtcggcgac gatcttgaac gcgagcgccg agaacggctc
     1561 gttctcgtcg gccttgcgct cggtcggctc gcccttcatg tcgacaccct cggtcggcgg
     1621 gatgtcgagg ggcgaaggga ggtagtcgac gaccgcgtcg agcatgggct gcacgccctt
     1681 gttcttgaac gcggaaccgc agatcacggg cacgaactcg aacgcgaggg tgcccttgcg
     1741 caccgcgcgc tggatctcct ccggggagat ctcgtgctcc tcgaggtact tctcgagcag
     1801 gtcgtcgtcg acgtgcgcga tcacttcgag caggcggccg tgggcctcct gcgcgtgctc
     1861 gagcaggtcg gcggggatgt cggtgacttc gaagttcgcg ccttcggagt cgtcgtcgag
     1921 cggccagacc aacgccttca tggtgatgag gtcgatgacg ccgcggaacg tgccctcggc
     1981 gccgatcggc agctgcaaca cgacgggcac cgcgctcaag cgctcgcgga tcatctgcac
     2041 gcagcgctcg aagtcggcgc cgacgcggtc catcttgttg acgaaacaga tgcgcggcac
     2101 ggagtacgtg ttggcctgtc gccacacggt ctcggtctgc ggctcgacac cggcgaccgc
     2161 gtcgaacacc gcgaccgcgc cgtcgaggac gcgcagcgac cgctcgacct cgaccgtgaa
     2221 gtcgacgtgg ccgggcgtgt cgatgatgtt gacctggcag tccttccact gtgcagtcgt
     2281 cgcggccgac gtgatcgtga tgccgcgctc ttgctcctgc accatgtggt ccatgaccgc
     2341 ggcgccttcg tgcacctcac cgatcttgta ggtgcgaccg gtgtagtaga ggatccgctc
     2401 ggtcgtcgtg gtcttgcccg cgtcgatgtg ggccatgatc ccgatgttgc gagtcttttc
     2461 gaggggaaat tcgcgtgcca tctctttgct tctctttgtg gggaagtcgg ggggttgcag
     2521 cagtcgaccg ggccctggga gcccggtcgg gagtcgcggg tcgctgcccg ctacgcgtca
     2581 gttgctggct agcggcctga cgcgctcccg tctgtcactg ctcggatcac catgtctcaa
     2641 tcgacccccg cggacgcatt accagcggta atgcgcaaaa gccttgttgg attcggccat
     2701 cttctggagg tctt
//
LOCUS       DASUGA010000095         6153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1646620, whole genome shotgun sequence.
ACCESSION   DASUGA010000095 DASUGA010000000
VERSION     DASUGA010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646620"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VFX21_01760"
     CDS             complement(<1..481)
                     /locus_tag="VFX21_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094704.1"
                     /translation="MTAFEPPGNGEWRSFAEHLPHPIARVFEQLFVDTIADGMGRAFV
                     RYGIPVKTITLRFVHGHAYLAPAPLMESKGRALPPKPILWIAARVHPEFRRREKAARN
                     ALATRIWRQDTARWFDELRDVWIERNRALQAVDPAGLADDELAVHTRECFENVIAGYK
                     "
     gene            complement(525..1697)
                     /locus_tag="VFX21_01765"
     CDS             complement(525..1697)
                     /locus_tag="VFX21_01765"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX5094705.1"
                     /translation="MSHDETAKEEPAPTGTPRRGFLVRAALVASVAAIVLALAFGEYL
                     ATRDGDKTPTTELAAATSPSTGAATTATTEATTTTTIPPTTTTTIPPLVQQAPLMLPP
                     PPGGAAKSGNSGNEIWAYEARMQQLHFDPGPVDGKFDGKTKYAVEAVQKQYNLPRTGV
                     VDQATVDALTNFQYTPLVPAGEKDRVEIDLDKQVLTVYRDWQVVLVTTTSTGSGKRFC
                     GGSDGCQYAVTPPGKYAFTWHHNGWRDGDLGRLYNPWYFNGGIAVHGYSSVPTTPASH
                     GCSRIPMHIAEYFSNLVYKDMTVYVLGTEAPRTGSGGGGGSSPATTAPTTVAPVPDPN
                     ATTVPPAPDPNATTVPPVTEAPTTVAPAVDPNTTTVPIAPGPSTPAPPVPPAPPAP"
     gene            complement(1766..4090)
                     /locus_tag="VFX21_01770"
     CDS             complement(1766..4090)
                     /locus_tag="VFX21_01770"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX5094706.1"
                     /translation="MSAVDPRTMLGRLGGAAPIGLAAFDRDMRFLTVNSWLARLNGYA
                     IHEHVGRTLHEIVPTIADRIEDVVVQVLERRRPVYGVEMTRPHSQTGAVVHAEATFFP
                     LIDGTGETVGVGCVVIDTTDRLLAIRRISLLQEAVNLATTAPDPTTAAQAIVERVLDA
                     VQAQGVGIAFPSGDFLQYAAAAGPLGATVTTMFPRLKISDAHGPIGEAFRERRTVWCA
                     TKEDWQRQYPESKMFEAGARAVLAIPLASATGGVLGVLGVAFDHEAYLTEDDRDLVTA
                     FTQQAAQALERVMLYQIQLDVRDRLALLASLGGRLDDSIEIEERVETFLSVVVPRFAG
                     IASVELVATEEYTSPLREERVADGMDVAELELMTVPLVARGEVFGVVSFGRAQFTETD
                     RDLARELVRRLAGALENARLYARERHIAETLQLSLLPRHIPAVDGVRLWARYLPGTDL
                     VVGGDFWDVIELPFQRVLLVVGDVAGRGERAAVVMGRLRTIIQAESDEHDSPATLLHT
                     LNRFLVEHEDEMATAICAVLDLTSGKVQLANAGHPPILRVTPAGETEYLGNATGLPLG
                     VRPFANYTEVSFHLAIGDTLLLFTDGLVERRGEDIDDRFAELAGAAAEVATKGELWCD
                     RVVERMIGRRRDDDVALLGVRYVGALTPELSIDCPAELVHLKAVRDRVRTWLTLQDAT
                     PDDVDAVLLAVGEAVANVAMHAYGARGGRLRVHGSLEDGVVHMRVDDDGQWRPPPDDL
                     GRGMHIVEQMSDTVSVDARDDGTTVTFTRRLGAL"
     gene            complement(4146..4319)
                     /locus_tag="VFX21_01775"
     CDS             complement(4146..4319)
                     /locus_tag="VFX21_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094707.1"
                     /translation="MDQAGLVTLIDDALERFAVRDLVSSGEVVDFLLDLRTAVVSDAA
                     LAALIEAESQPTG"
     gene            complement(4511..4996)
                     /locus_tag="VFX21_01780"
     CDS             complement(4511..4996)
                     /locus_tag="VFX21_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX5094708.1"
                     /translation="MAGLEDLYREIILDHYRSPRNRGELAVPPAHKSEGFNPLCGDEV
                     VLYLVVDPATDTVTDIKIAGQGCSISQASTSMMSAAVKGKPLDEVHQLIRAFKALMSV
                     HESKLEGESDGSDLAADLEGVRLGDLEALQGVVKFPVRIKCATLAWNTLQQGLEEIAH
                     A"
     gene            complement(5020..>6153)
                     /locus_tag="VFX21_01785"
     CDS             complement(5020..>6153)
                     /locus_tag="VFX21_01785"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HEX5094709.1"
                     /translation="QKPAAVLDAMDECYRNYYANVHRGVYSIAEKATATMEAARARVA
                     RFVNARAASEIVFVRNATEAINLVAYSWGRHNLREGDVVVLSHMEHHANVVPWQMLAD
                     ERGIELRWIPLTDDFRLDLTELDALLDGARLLAISAMSNVLGTINDIRPLADAAHAHD
                     ALVLVDACQYVPHVRTDVQAWDADFVAFSAHKMCGPSGIGALWARHELLDAMPPFLGG
                     GEMIRDVRLDGFTTNDVPWKFEAGTPAIVEIVGFGAAVDYLEGIGMESVRNHEMRLTR
                     YALDRLRERFPDSLTIYGPSDVSIRGGAVSFLFDGIHAHDISQVLDEDAVCVRAGHHC
                     AKPLMRQLGVPATSRASFYLYNEEQEVDVLVEALAKAEKFFRI"
BASE COUNT          895 a         2030 c         2233 g          995 t
ORIGIN      
        1 gcttgtatcc ggcgatgacg ttctcgaagc attcgcgggt gtgcaccgcg agctcgtcgt
       61 cggcgagacc ggccggatca acggcttgca gcgcgcgatt gcgttcgatc cacacgtcgc
      121 gcagctcgtc gaaccagcga gccgtatctt gtcgccagat gcgcgtcgcg agcgcgttgc
      181 gagccgcctt ctcgcgccgg cggaactccg ggtgcacgcg cgccgcgatc cacaggatcg
      241 gtttgggcgg gagcgcccgg cccttgctct ccatgagcgg tgccggcgcc aggtacgcgt
      301 ggccgtgcac gaatcgcaac gtgatggtct tgaccggaat gccgtagcgc acgaaggcgc
      361 ggcccatccc gtccgcgatc gtgtcgacga agagctgttc gaacacgcgt gctatcgggt
      421 gcggaaggtg ctccgcgaac gaacgccact cgccgttgcc cggaggctcg aacgcggtca
      481 tcccgccatg atccccgaga acgcccaggt cggggcgaat ccggtcaggg tgcgggcggt
      541 gcgggcggta caggcggtgc aggcgtcgac gggccgggtg cgatcgggac ggtggtggtg
      601 ttcgggtcga ctgccggcgc gacggtcgtc ggcgcctcgg tcacgggcgg cacggtggtc
      661 gcgttggggt cgggtgcggg cggcacggtc gtggcgttcg ggtcgggcac gggtgcaacc
      721 gtggtcggcg cggtcgtcgc cgggctcgag cctccgccgc cgcccgaccc cgtgcgcggc
      781 gcctcggtgc cgagcacata cacggtcatg tccttgtaca cgaggttgga gaagtactcg
      841 gcgatgtgca tcgggatgcg cgagcagccg tgcgacgcgg gcgtggtggg cacggacgag
      901 tacccgtgca ccgcgatgcc gccgttgaag taccaggggt tgtagaggcg accgaggtcg
      961 ccgtcgcgcc aaccgttgtg gtgccacgtg aatgcgtact tgcccggtgg tgtgaccgcg
     1021 tactggcaac cgtcgctccc gccgcagaag cgcttgccgc tccctgtcga cgtcgtcgtg
     1081 acgagcacga cctgccagtc gcggtagacc gtgagcacct gcttgtcgag gtcgatctcg
     1141 acgcggtcct tctcgcccgc gggcacgagc ggtgtgtact ggaagttcgt gagcgcgtcg
     1201 acggtcgcct ggtcgacgac gccggtgcgc ggcaagttgt actgcttctg cacggcttcg
     1261 accgcgtact tcgtcttgcc gtcgaacttg ccgtcgaccg gacccgggtc gaagtgcagt
     1321 tgttgcatcc gtgcctcgta cgcccagatc tcgttgccgc tgttcccgga cttcgcggcg
     1381 ccgcccggcg gcggcggcaa catcaacggc gcctgctgga cgagcggcgg gatcgtcgtg
     1441 gtcgtggtcg gcgggatcgt cgtggtcgtc gtcgcctcgg tcgttgccgt cgtcgcggcg
     1501 ccggtcgacg gagaggtggc ggccgctaac tcggtcgtcg gcgtcttgtc gccgtcgcgc
     1561 gtcgcgaggt actcgccgaa cgcgagcgcg aggacgatcg cggcaacgct cgcgacgagt
     1621 gcggcccgca ccaagaaccc acgacgcggc gtgcccgtcg gagccggctc ctccttggcc
     1681 gtttcgtcgt gggacactgc ctcgatgcta ctggaccgtt tcccgagcaa ggggacgcgg
     1741 ctatgcggac cggtcgggag gtccgtcaga gcgcgccgag tcggcgcgtg aacgtcaccg
     1801 tggtcccgtc gtcacgcgcg tcgacggaga ccgtgtcgct catctgttcg acgatgtgca
     1861 tgccgcgacc gagatcgtcg ggcggcggtc gccactgacc gtcgtcgtcg acgcgcatgt
     1921 gtacgacgcc gtcttccaac gatccgtgca cgcgcaagcg cccgccccgc gccccgtacg
     1981 catgcatggc gacgttcgca acggcttcac cgaccgccag caacacggcg tcgacgtcgt
     2041 ccggagtggc gtcttgcaat gtcagccacg tgcgcacgcg gtcgcgcacg gccttgaggt
     2101 gcaccagctc ggccgggcag tcgatcgaca gctcgggtgt gagcgcgccg acgtaccgga
     2161 cgccgagcag cgcgacgtcg tcgtcgcgcc ggcggccgat catccgctcg acgacgcgat
     2221 cgcaccacag ctcgcccttc gtcgcgactt cggcggcagc acccgccagc tccgcgaagc
     2281 ggtcgtcgat gtcctcgccg cggcgttcga cgagcccgtc ggtgaacaac aacagcgtgt
     2341 cgccgatcgc gaggtggaat gacacctcgg tgtagttcgc gaacgggcgc acgccgagcg
     2401 gtaatccggt tgcgttgccg aggtactcgg tctcgccggc cggcgtgacg cgcaggatcg
     2461 gcgggtgacc ggcgttcgcg agttgcacct tgccgctggt caggtcgagc accgcgcaga
     2521 tcgcggtcgc catctcgtcc tcgtgctcga ccaggaagcg gttcagcgtg tgcagcagag
     2581 tggcgggcga gtcgtgctcg tccgactcgg cctggatgat cgtgcgcagc cggcccatga
     2641 cgacggcggc ccgctcgccg cggcccgcga cgtcgccgac gacgagcagg acccgctgga
     2701 acggcagctc gatgacgtcc cagaagtcgc cgccgacgac gaggtcggtg ccgggcaggt
     2761 accgggccca cagccgcaca ccgtcgacgg ccgggatgtg gcggggcagg aggctgagct
     2821 ggagcgtctc cgcgatgtgc cgttcgcgtg cgtacaggcg cgcgttctcg agcgcgcccg
     2881 cgagccgccg gacgagctcg cgcgcgaggt cgcggtcggt ctcggtgaac tgcgcgcgcc
     2941 cgaacgagac gacgccgaat acttcgccgc gcgcgacgag cgggacggtc atcaactcga
     3001 gctcggcgac gtccatcccg tccgcgacgc gctcctcgcg gagcgggctg gtgtactcct
     3061 cggtcgcgac gagctcgacg ctcgcgatgc cggcgaagcg tgggacgacg acgctgagga
     3121 acgtctcgac gcgctcttcg atctcgatgc tgtcgtcgag gcggccgccg agcgacgcga
     3181 gcagcgcgag acgatcgcgc acgtcgagct ggatctggta cagcatgacc cgctcgagcg
     3241 cctgcgcggc ctgctgggtg aacgcggtca cgaggtcgcg gtcgtcctcg gtgaggtatg
     3301 cctcgtggtc gaacgcgaca ccgagcacac cgagcacccc gccggtcgcc gacgcgagcg
     3361 ggatcgcgag cactgcacgc gcgcccgcct cgaacatctt cgactcggga tattgccgct
     3421 gccagtcttc cttcgtcgcg caccagacgg tgcggcgctc acggaaggct tcgccgatcg
     3481 gtccgtgcgc gtcgctgatc ttgagccgcg ggaacatcgt cgtgacggtc gcgccgagcg
     3541 ggccggccgc cgcggcgtat tggaggaagt cgccggacgg gaacgcgatg ccgacgccct
     3601 gcgcttggac tgcgtcgagg actcgctcga cgatcgcctg ggcggccgtg gtcgggtcgg
     3661 gtgccgtggt cgcgaggttc acggcttcct gcaacaggga gatgcgccgg atggccagca
     3721 gacgatcggt cgtgtcgatg acgacacagc cgacgccgac ggtctcgccg gtgccgtcga
     3781 tcaacgggaa gaacgtcgct tcggcgtgga cgacggcgcc ggtctggctg tgcgggcgcg
     3841 tcatctcgac gccgtagacc ggccgccggc gttcgagcac ttggacgacg acgtcctcga
     3901 tgcgatcggc gatggttggg acgatctcgt gcagcgtgcg cccgacgtgc tcgtggatcg
     3961 cgtagccgtt caggcgcgcc aaccagctgt tgacggtcag gaaccgcatg tcgcggtcga
     4021 acgcggccag cccgatgggg gcggcaccgc ccaaccgtcc gagcatggtg cgcgggtcga
     4081 cggccgacat cggccgcgaa gctaacggga atggtgggcc gatatcaccc gcccgcgagg
     4141 tgcgctcagc cggtcggctg ggactcggcc tcgatgagcg cggcgagtgc ggcgtcggag
     4201 acgaccgcgg tacgcaggtc gagaaggaag tcgacgacct cgcccgacga gacgagatcg
     4261 cggaccgcga accgctccag tgcgtcgtcg atcagtgtga cgagcccggc ctggtccatg
     4321 tcgagtcctc ctcggggtac gtagatgcag cgtagtcggc gcgtgcacct acccatacaa
     4381 cgtccgggag ccccgtttgg tttcccccgg cgcacgaact ggtgacggat gacgcacacg
     4441 ggcacacacg aggcagatga ggtgtgcgac gggcgtcacc agcggtcgcg gggtccggcg
     4501 gccggggtga ctaggcgtgc gcgatctcct cgagcccctg ctggagggtg ttccacgcga
     4561 gagtggcgca tttgatgcga acggggaact tgaccacgcc ctgcaatgcc tcgaggtcgc
     4621 cgaggcgcac gccttcgagg tccgcggcga ggtcgctgcc gtcgctctcg ccctcgagct
     4681 tggattcgtg cacggacatc agcgccttga acgcgcggat gagctggtgc acttcgtcga
     4741 gcggcttgcc cttcaccgcc gcgctcatca tcgacgtcga cgcctggctg atcgagcagc
     4801 cctggcccgc gatcttgatg tcggtgacgg tgtcggtcgc ggggtcgacg acgaggtaga
     4861 ggaccacttc gtcgccgcac aacgggttga agccctcgga cttgtgcgcg ggtggtaccg
     4921 cgagctcgcc gcggttacgc ggcgatcggt agtgatcgag gatgatctcc cggtagaggt
     4981 cttcgagacc ggccatcgcg tgttgatcct acgtcgcggt cagatgcgga agaacttctc
     5041 ggccttggcc aacgcctcga cgagcacgtc gacttcctgc tcctcgttgt agaggtagaa
     5101 ggacgcccgg ctggtcgcgg gcacgcccag ctggcgcatg aggggcttgg cgcagtggtg
     5161 cccggcgcgc acacagacgg cgtcctcgtc gagcacctgg ctgatgtcgt gggcgtggat
     5221 gccgtcgaac aagaacgaca ccgcgccgcc gcgaatcgag acgtcggagg ggccgtagat
     5281 cgtcaacgag tcggggaacc gctcgcggag ccggtcgagc gcgtaccgcg tgagccgcat
     5341 ctcgtggttg cgcacggact ccatgccgat gccttcgagg tagtcgactg cggcgccgaa
     5401 gcccacgatc tcgacgatcg ccggtgtgcc ggcttcgaac ttccacggga cgtcgttcgt
     5461 ggtgaagccg tcgagacgga cgtcgcggat catctcgccg ccgccgagga agggtggcat
     5521 cgcgtcgagc agctcgtggc gggcccacag tgcgccgatg ccggacggcc cgcacatctt
     5581 gtgcgcggag aacgcgacga agtcggcgtc ccacgcttgg acgtcggtgc gcacgtgcgg
     5641 cacgtactgg cacgcgtcga cgaggacgag cgcgtcgtgc gcgtgcgcgg cgtccgcgag
     5701 cgggcggatg tcgttgatgg tgccgagcac gttcgacatc gcgctgattg ccagcagccg
     5761 cgcgccgtcg agcaacgcgt cgagttcggt gagatcgagg cggaagtcgt cggtgagcgg
     5821 gatccagcgc agctcgatgc cgcgctcgtc ggcgagcatc tgccacggca cgacgttcgc
     5881 gtggtgctcc atgtgcgaga gcacgacgac atcgccctcg cgcaggttgt gccggcccca
     5941 gctgtacgcg acgaggttga tcgcctcggt cgcgttgcgc acgaacacga tctccgaagc
     6001 cgcccgtgcg ttcacgaagc gggcgaccct cgctcgtgcg gcctccatcg tcgcggtggc
     6061 cttctcggcg atcgagtaca cgccgcggtg cacgttcgcg tagtagttgc ggtagcactc
     6121 gtccatcgcg tcgagcaccg ccgccggctt ctg
//
LOCUS       DASUGA010000096         2465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1784288, whole genome shotgun sequence.
ACCESSION   DASUGA010000096 DASUGA010000000
VERSION     DASUGA010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1784288"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VFX21_01790"
     CDS             complement(<1..508)
                     /locus_tag="VFX21_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5094710.1"
                     /translation="MTVRVRPLECGWLTSDAAGMVTGQTGRMRMPVPAFLVEHERGTM
                     VFDAGMHPELMERTDRLRHTAELFELEYTADSTLAAQLAGAGVDATAVPIAAVSHLHF
                     DHCGGLVGVPNARVLVQQAEWDAAFESGLVEFGVFNPDDFDVGHDRVLLDGAHDVFGD
                     GSVVLVPTP"
     gene            complement(505..1662)
                     /locus_tag="VFX21_01795"
     CDS             complement(505..1662)
                     /locus_tag="VFX21_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEX5094711.1"
                     /translation="MGFPSEFWWGTGASSTQCEGAAPASDWLRMEREGRVPPSGDGNG
                     FGTRYAEDFELYAAYGLTHHRLSIEWARIEPEEGKRDDAAIEHYIRVLEAARAVGVSP
                     WVCLHHFTLPGWFTEIGEGGFVDDRARSYYWPRHVAFCAETFGDLVYGWKPINEPGAF
                     AAIYSQRARRFDVLGAILLAQRDAWRELRGGGKPVSTVHNLSPLFGSSSTAEQMIWKV
                     WMRADRDGVLELPGRVPREVPDLKEACDLIGFSYYSALGFDAEGKIVPYPPDARVGPM
                     GYAPWSEGLGIVIRRLHDELPGRPLLVCEHGVGTDDDEWRCDILRESLAIVEEAVDDG
                     IDLRGFFHWTGVDNYEWNHGYTVQFGLFDRNREPRGSAELMREYATRRSPA"
     gene            1692..>2465
                     /locus_tag="VFX21_01800"
     CDS             1692..>2465
                     /locus_tag="VFX21_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094712.1"
                     /translation="MSRFRYSRWDGTQVGFDLDADALLDEMTDDLLYHGDLNAALRRM
                     MQQGFQDREGRDVQGMRDMLEQLRQRRREQLERYDLGGVYEEIAQRLDEILEEERAGI
                     ERRLDDARQSGDQRREELLEDLARDRREQLEQLPPDLAGRVSDLQQYDWMDDTARQKF
                     EELMEELKKQLLDSMFNQLQQGLSEMSPEQLARMKDMLAELNHMLEQRERGEEPDFEG
                     FMERFGDFFPENPQSLDELLEVMAQRMAAMQAMLNSMTPE"
BASE COUNT          440 a          856 c          818 g          351 t
ORIGIN      
        1 cgggtgtcgg cacgaggacg accgaaccgt cgccgaacac gtcgtgcgcg ccgtcgagca
       61 gcacgcggtc gtggccgacg tcgaaatcgt cggggttgaa cacgccgaac tcgaccaaac
      121 ccgattcgaa cgccgcgtcc cactcggctt gttggacgag cacgcgcgcg ttcgggacgc
      181 cgacgagacc gccgcagtga tcgaagtgca gatggctcac tgccgcgatc ggcaccgcgg
      241 tcgcgtcgac gcccgcgccg gcgagctgcg ccgccaacgt cgaatcggcg gtgtactcga
      301 gctcgaacag ctcggccgtg tgacggagcc gatcggtgcg ctccatgagc tccggatgca
      361 tgcccgcgtc gaacaccatc gtgccgcgct cgtgctcgac gaggaacgcg ggcaccggca
      421 tgcgcatgcg gccggtctgg cccgtcacca ttcccgcggc atcggaggtc agccaaccgc
      481 attcgagcgg gcgcactcgc actgtcatgc cggcgacctc cgcgtcgcgt actcgcgcat
      541 cagttccgcg ctgccgcgtg gctcgcggtt gcggtcgaac aacccgaact gcacggtgta
      601 accgtgattc cactcgtagt tgtcgacgcc ggtccaatgg aagaagccgc gcaggtcgat
      661 gccgtcgtcg acggcttcct cgacgatcgc gagtgactcg cgcaggatgt cgcagcgcca
      721 ttcgtcgtcg tcggtcccga cgccgtgctc gcagaccaac aacggccggc ccggcagctc
      781 gtcgtgcagc cggcggatga cgatgcccag cccttcgctc cacggcgcgt accccatcgg
      841 cccgacgcgc gcgtccggcg ggtaggggac gatcttgcct tcggcgtcga agcccagcgc
      901 ggagtagtag gagaacccga tgaggtcgca cgcctccttc aggtcgggca cctcgcgcgg
      961 cacgcggccg gggagctcga gcacgccgtc gcgatcggcg cgcatccaga ccttccagat
     1021 catctgctcg gccgtcgaag aagagccgaa gagcggcgac aggttgtgca ccgtcgacac
     1081 cggcttgccg ccgccgcgca gttcccgcca cgcgtcgcgt tgggcgagca ggatcgcgcc
     1141 gagcacgtcg aagcggcggg cacgttgcga gtagatggcc gcgaacgcac ccggttcgtt
     1201 gatcggcttc cagccgtaca cgagatcgcc gaacgtctcc gcgcagaacg cgacatgacg
     1261 cggccagtag taggagcgcg cgcggtcgtc gacgaagccg ccttcgccga tctcggtgaa
     1321 ccaacccggc agcgtgaagt ggtgcaggca cacccacggc gacacgccca cagcgcgcgc
     1381 cgcttcgagc acacggatgt agtgctcgat ggcagcgtcg tcgcgcttgc cctcttcggg
     1441 ctcgatgcgc gcccactcga tcgacaggcg atggtgcgtc agcccgtacg ccgcgtacag
     1501 ctcgaagtct tcggcgtagc gcgtgccgaa cccgttgccg tcacccgacg gcggtacccg
     1561 gccctcgcgt tccatccgca gccagtccga cgcgggcgcc gcgccttcgc actgtgtcga
     1621 cgaagcgccg gtgccccacc agaactcgga ggggaaaccc atggttcgga ccataggact
     1681 accgtgctcg catgagcagg ttccggtact cgcggtggga cggcacgcag gtcgggttcg
     1741 acctcgacgc cgacgcactc ctcgacgaga tgaccgacga cctgctctac cacggcgacc
     1801 tcaacgccgc gctgcggcgg atgatgcagc aggggttcca ggaccgcgaa ggacgcgacg
     1861 tccagggcat gcgcgacatg ctcgagcagc tccgccagcg gcgccgtgag caactcgagc
     1921 gctacgacct cggtggcgtc tacgaagaga tcgcgcaacg gctcgacgag atcctcgaag
     1981 aagagcgcgc cggcatcgaa cgccgcctcg acgacgcgcg ccagtcgggc gaccagcggc
     2041 gcgaggaact cctcgaagac ctcgcgcgcg accggcgtga acaactcgaa cagctgcccc
     2101 ccgatctcgc gggccgcgtc agcgacctgc agcagtacga ctggatggac gacaccgcgc
     2161 ggcagaagtt cgaagagctg atggaggagc tgaagaagca gctcctcgac tcgatgttca
     2221 accagttgca gcaagggctc tcggagatgt cacccgagca gctcgcgcgt atgaaggaca
     2281 tgctcgccga gctcaaccac atgctcgaac agcgcgagcg cggcgaggaa ccggacttcg
     2341 aagggttcat ggagcgcttc ggcgacttct tccccgagaa cccgcagtcg ctggacgagc
     2401 tgctcgaggt gatggcgcag cgcatggcgg ccatgcaggc catgttgaac tccatgacac
     2461 ccgag
//
LOCUS       DASUGA010000097         2077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1503367, whole genome shotgun sequence.
ACCESSION   DASUGA010000097 DASUGA010000000
VERSION     DASUGA010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503367"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VFX21_01805"
     CDS             <1..496
                     /locus_tag="VFX21_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094713.1"
                     /translation="ITHAVASHEDGIGMFAWRNDDGTWSPFFPNGPLMFSQREFDAIV
                     RGEHPSEGRVLRELHAKGAVQLTGDRTQITDAVVVEHTGAHTPGHQVVRIDSNGARAI
                     FVGHLAVSPLHFVSGECPPLNLDPERAWTVLTALRDEGALLIGPLWPEPGAGRWDCSQ
                     LVPV"
     gene            complement(504..1649)
                     /locus_tag="VFX21_01810"
     CDS             complement(504..1649)
                     /locus_tag="VFX21_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094714.1"
                     /translation="MRRRALLAAGVIAIASACSGGGSSTDATVRVTTSKRAPHKLRAT
                     STTSTSTSTTTSTSTTTTTVAPAPPPTARRTPAPTAAPPVAAPPTSPSALPPSTGGAP
                     VIDGCGVFPADNAWNRDVSTLPVHPNSANLIASINASRTTLHADFGGGGEYGIPFVTV
                     ADSEPMQNVNWTAYGDESDPGPYPIPPTAPVEGGADADGDRHVIALQRGTCKLFELYR
                     AFWQGDHWDADSGAVYDLRSNALRPLGWTSADAAGLPILAGLVRYDEVAAGSVNHALR
                     FTVQRTQRGYILPATHAASSSNDANLPPMGLRLRLRADFDTSGYSGQARVILEGLKRY
                     GMIVADNGSSWYITGAADPRWTDDDLDQLKRVPGSAFEVVDTGPIQR"
BASE COUNT          288 a          712 c          722 g          355 t
ORIGIN      
        1 catcacgcac gccgtcgcat cgcacgaaga cggcatcggc atgttcgcgt ggcgcaacga
       61 cgacggcacc tggtcgccgt tctttcccaa cggaccgctg atgttctcac agcgggagtt
      121 cgacgcgatc gtgcgtggcg agcatccgag cgagggccgg gtgctgcgcg agctgcacgc
      181 caagggcgcc gtgcaactca cgggcgatcg cacgcagatc accgacgcgg tagtcgtcga
      241 gcacacgggt gcacacacac ccggccatca agtcgtgcgc atcgactcga acggagcgcg
      301 cgcgatcttc gtcggccatc tcgcggtcag cccgcttcac ttcgtgagcg gtgagtgtcc
      361 gccgctcaac ctcgacccgg aacgcgcctg gacggtgctc actgcgctgc gcgacgaagg
      421 cgcgctgctc atcggtccgc tgtggcccga gcccggcgcc ggccgttggg actgcagcca
      481 actcgtgccc gtgtgaggtt cgctcagcgt tggatcgggc cggtgtcgac gacttcgaac
      541 gcgctgccgg gcacgcgctt gagttggtcg aggtcgtcgt cggtccagcg cgggtcggcg
      601 gcgccggtga tgtaccacga cgatccattg tcggcgacga tcatcccgta gcgcttcagt
      661 ccctcgagga tcacccgcgc ctggccggag tatcccgacg tgtcgaagtc ggcgcgcagt
      721 cgcaggcgca gacccatcgg cggcaagttg gcatcgttgc tcgacgacgc ggcgtgcgtc
      781 gcgggcagga tgtagccgcg ctgcgtccgt tgcaccgtga agcgcagtgc gtggttcacc
      841 gagccggccg ccacctcgtc gtaacgcacg agacccgcga ggatcggcag ccccgccgcg
      901 tcggccgacg tccaaccgag tggtcgcaac gcgttcgacc gcaggtcgta caccgcgccc
      961 gaatcggcgt cccagtggtc gccttgccag aaggcgcggt agagctcgaa cagcttgcag
     1021 gtcccgcgct ggagcgcgat gacgtggcgg tctccgtcgg cgtccgcgcc gccttcgacc
     1081 ggggcggtcg gcgggatcgg atacggaccg ggatcgctct cgtcgccgta cgcggtccag
     1141 ttgacgttct gcatcggttc cgagtcggcg accgtcacga acgggatgcc gtactcgcca
     1201 ccgccgccga agtcggcgtg caacgtcgta cgcgacgcgt tgatgctcgc gatgaggttc
     1261 gcagagttcg gatgcacggg caacgtcgac acgtcacggt tccacgcgtt gtcggccgga
     1321 aacacaccac aaccgtcgat gacgggcgcg ccgccggtgc tcggcggcag cgcggacggt
     1381 gacgtcggtg gagcagcgac gggcggcgcg gccgtcggcg ccggagtacg gcgcgcggtg
     1441 ggtggcggtg ccggcgcgac cgtggtggtc gtggtgctcg tgctcgtcgt agtgctcgtg
     1501 ctcgtgctcg tcgtcgacgt cgcgcgcagc ttgtgcggcg cgcgcttcga cgtagtgacg
     1561 cgcacggtcg cgtcggtcga cgagccgccg cccgagcacg cgctcgcaat cgcaattacc
     1621 cccgcggcga ggagggcccg acgtcgcatc tcgtcccctt ccgtgggtca gatcggccgt
     1681 cgccgtctcc gaactgtaat tctttgtcgc tccggtcgtg ctcgcgtgag gcagcccgga
     1741 ttgttggccc gccctccgct ccggcgagtg cctcggcaag ctccggcacc gcttctcttt
     1801 gtcgccttcg acctactcgc gtgaggcggc ccggattgct ggccggccgc aggactccgc
     1861 gagtgcctcc gcaagctccg gcaccgcgcg gttgatgctc gcgcgtcacg cgcccggcag
     1921 tggaccttcg tacttgtcga gcactctctt gggggctttg acgcgccagg actcggtgat
     1981 cagctcgcgc agctcgcgga cgccgacctt cttcacgttc acgagcacgg tcgcgtagcc
     2041 gtcgtagtgc ggcgtcgtga agaacttctt cgaatcg
//
LOCUS       DASUGA010000098         2700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1920733, whole genome shotgun sequence.
ACCESSION   DASUGA010000098 DASUGA010000000
VERSION     DASUGA010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1920733"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..94)
                     /locus_tag="VFX21_01815"
     CDS             complement(<1..94)
                     /locus_tag="VFX21_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017612198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5094715.1"
                     /translation="MIREATPGDVDEILAMIRELAEYEREIDAVV"
     gene            complement(91..1149)
                     /gene="gcvT"
                     /locus_tag="VFX21_01820"
     CDS             complement(91..1149)
                     /gene="gcvT"
                     /locus_tag="VFX21_01820"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX5094716.1"
                     /translation="MRHSPLDAVHRSLGARMVPFGGWEMPLQYTGVLEEHRACREHAV
                     VFDVSHLGRVGLAGATPGDGKRALQWMLTNDLDKIDVGRAQYTHLLDADDAHVVDDII
                     VWWEFEDQFVVMPNASNTDGIMAVFGEAANRFDIAYAEQRGAVLAVQGPEARALLEKV
                     NPDWAAVPRFHVSMVQGGVAAGTGYTGEDGIELLVAPEDAPAIWRSLLDAGIVPAGLG
                     ARDTLRLEAGLPLHGHELGPGITPLQAGLGWVVGWDKDDFRGRAPLAAERERGIARRL
                     RGLSIEGRRPARDGAPVVRDGEAVGVVTSGNFSPTLGHAIALAFVPPEVGIGDALQVD
                     VRGTLLDAEVVKLPFVSK"
     gene            complement(1162..1620)
                     /locus_tag="VFX21_01825"
     CDS             complement(1162..1620)
                     /locus_tag="VFX21_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094717.1"
                     /translation="MALAAEVLPHEEVRFAHNSERQFAKLLDFYGIAWDYEPRTFTLE
                     IDRDGNPQQAFTPDFYLPAYDLYIEITTLNQKLVTKKNRKARRLAELYPDVSIRVLYQ
                     RDYLHLLVKYGLEPPSQLVEAGVDGVTPLPFDLGRRAGGSPAPSPTAETA"
     gene            complement(1626..2072)
                     /locus_tag="VFX21_01830"
     CDS             complement(1626..2072)
                     /locus_tag="VFX21_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014692028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5094718.1"
                     /translation="MTSAAHEPGYRGPQVCKIVGITYRQLDYWARTDLVRPSVMDANG
                     SGTQRLYSFRDMLALKVIKQMLDAGIELKSARKAIESLRSFGGDPASAFIAVDGKQAI
                     LLDEDASLDLMRNGQGVFSFVPLKPAVQQINEAVQLSFDDAKAAGQ"
     gene            complement(2137..>2700)
                     /locus_tag="VFX21_01835"
     CDS             complement(2137..>2700)
                     /locus_tag="VFX21_01835"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX5094719.1"
                     /translation="DRDFAIVHGSCTEDDTFREAGIERARAVIVAVGDDAVAMSATLS
                     ARALNPKLLIIARANDVSSEAKLVRAGCDRVVNPLSQGAHRMAAFAQTPDVADFLDVV
                     VHDADVEYRLEEYRVPEHCRLRGKTLGDAHIRRESGALVLALRDGAGSFQSNPPPDVV
                     LAAGITIIAIGTSDQLNKLEGLLQPEG"
BASE COUNT          456 a          923 c          856 g          465 t
ORIGIN      
        1 caacgacggc atcgatttcg cgctcgtact ccgcaagctc gcggatcatc gccagtatct
       61 cgtcgacatc ccccggagtg gcttcgcgaa tcatttcgac acgaacggca gcttgacgac
      121 ctctgcatca agcagcgtcc ctcgcacgtc gacctgcagc gcatcgccga tcccaacctc
      181 cggcggcacg aacgcgagcg cgatggcgtg cccaagtgtc ggcgagaagt tgccactcgt
      241 caccactccg accgcttcac cgtcgcgcac gacgggtgcg ccatcgcgcg cgggccggcg
      301 gccctcgatg gagaggccgc gcaaacgacg cgcgatcccg cgttccctct ccgcggcgag
      361 cggcgcccgc ccgcggaagt cgtccttgtc ccagccgacg acccatccga gtccggcctg
      421 cagcggtgta attccaggac cgagttcgtg gccgtgcagc ggcaggccgg cttcgagccg
      481 cagcgtgtcg cgagcaccca aaccggcggg cacgatgccc gcgtcgagca gcgaccgcca
      541 gatcgccggc gcgtcctcgg gcgcaaccag aagttcaatg ccgtcctcgc cggtgtagcc
      601 ggtgccggcc gccacgccgc cttgcaccat cgagacgtgg aatcgcggca ccgcggccca
      661 atccggattc accttctcga gcaacgcacg tgcttcgggg ccctgcaccg cgagaacggc
      721 gcctcgttgc tcggcgtagg cgatgtcgaa acggttggcc gcctccccga acaccgccat
      781 gatcccgtcg gtgttcgacg cgttgggcat gacgacgaac tgatcctcga actcccacca
      841 cacgatgatg tcgtcgacga catgcgcgtc gtccgcgtcg agcaaatggg tgtactgcgc
      901 tcgcccgacg tcgatcttgt cgaggtcgtt cgtcagcatc cactgcaacg ctcgcttccc
      961 gtcgccgggt gtcgcccccg cgaggccgac gcgtccgaga tgcgagacat cgaacacgac
     1021 ggcgtgttcg cgacacgcgc ggtgctcttc gagcacaccg gtgtattgca gcggcatttc
     1081 ccaaccgccg aacggcacca tgcgtgcgcc gagggagcgg tgaactgcgt cgagcggact
     1141 gtggcgcata cgcgcccgaa tctacgctgt ctcggcggtc gggctcgggg ccgggcttcc
     1201 gcccgcccgc cggccgagat cgaacggcaa cggtgtcacg ccgtcgacgc cggcctcgac
     1261 caattgtgac ggcggttcga ggccatactt caccaagagg tgcaagtagt cacgctggta
     1321 cagcacccgg atcgatacgt ccggatagag ctctgcgagc cggcgcgctt tgcggttctt
     1381 cttcgtcacc agcttctggt tcagcgtcgt gatctcgata tacaagtcgt aggccggaag
     1441 atagaagtcg ggagtgaagg cctgctgcgg attgccgtcg cgatcgatct cgagcgtgaa
     1501 cgtgcgcggc tcgtagtccc acgcaatgcc gtagaagtcg agcagcttcg cgaactgccg
     1561 ttccgagttg tgcgcgaagc gcacttcctc gtgcgggagg acttccgcag ccagagccaa
     1621 ctcccctact gccccgcggc cttcgcgtcg tcgaagctca gttgaaccgc ttcgttgatc
     1681 tgttgcaccg ccggcttcag cggcacgaac gagaacacac cctgaccgtt acgcatcagg
     1741 tcgagcgacg cgtcttcgtc gagcaggatc gcctgcttcc cgtcgaccgc gatgaatgcc
     1801 gatgccgggt cgccaccgaa cgaacgcaac gattcgattg ctttgcgcgc cgacttcagt
     1861 tcgatgccgg cgtcgagcat ctgcttgatg actttcaacg cgagcatgtc gcggaacgag
     1921 tagagccgtt gcgtgccgct gccgttcgcg tccatgaccg aggggcgcac gaggtcggtg
     1981 cgggcccagt agtcgagctg gcggtaggtg attccgacga tcttgcagac ctgcggtccc
     2041 cgataccccg gctcgtgcgc agcactcgtc atgacacaag tgtaactaca cgcaggtgaa
     2101 accgtaaacc tcgacctttt ccacaggtgc agaggctcaa ccttcgggct ggagcagacc
     2161 ctccagcttg ttcagctggt cactggtgcc gatcgcgatg atcgtgatgc cggccgcgag
     2221 caccacgtcg ggcggcgggt tcgactggaa gctcccggcc ccgtcgcgga gcgcgaggac
     2281 gagggctccg ctttcgcgcc ggatgtgagc gtcgcccaag gtcttgcccc gcagccggca
     2341 gtgctcgggc acgcggtact cctcgagccg gtattcgacg tcggcgtcgt gcacgacgac
     2401 gtcgaggaag tcggcgacgt cgggcgtctg cgcgaaggcg gccatgcggt gcgcaccctg
     2461 actcaacgga ttgacgacgc ggtcgcaccc ggctcgcacc agctttgcct cgctcgaaac
     2521 gtcgttggcg cgagcgatga tcaagagctt cggattgagc gcacgcgccg acagcgtggc
     2581 cgacatggca acggcgtcgt cgccgaccgc gacgatgacc gcccgtgccc gctcgatgcc
     2641 tgcttcgcgg aacgtgtcgt cttcggtgca gctgccgtgc acaatcgcga agtcgcgatc
//
LOCUS       DASUGA010000099         4586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1723345, whole genome shotgun sequence.
ACCESSION   DASUGA010000099 DASUGA010000000
VERSION     DASUGA010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1723345"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VFX21_01840"
     CDS             complement(<1..573)
                     /locus_tag="VFX21_01840"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5094720.1"
                     /translation="MTEPGPILASGRDGDVFEFGPGLVLRKTRDGRSIEHEARIIRYV
                     AAHGYPVPKIHDVRANGSEIVMDRIDGPLMMDTMLKPWKMPAMLRVLADLHDQLHEIA
                     APDHLRQMPDGGDRVVHLDLHPLNVIMAATGPVVIDWGNAARGEGMSDAALTYALLTC
                     PRVPGPKIVGTVLQPLRPLVGKQFARRWWSP"
     gene            609..1253
                     /locus_tag="VFX21_01845"
     CDS             609..1253
                     /locus_tag="VFX21_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094721.1"
                     /translation="MRDGLPRLVLVDGLPGSGKTTTAERMCRRLRSRGIDAAWAAETA
                     PDHPVFPRGFAKSRREPEFPQQCLDAWAAFVDTIGTRTWVLDGAAFQSTVRFFYDERV
                     PYDEIFAYWRAFETIVMPLRPTIFYLRDDNPADRMRTITIPVRGRAWYDKVSNYVART
                     PAGREFRHLGVEGFIAFWVRYGELCESLLAQTRLDVASSPLGLAPEPALVSDDA"
     gene            complement(1239..2789)
                     /locus_tag="VFX21_01850"
     CDS             complement(1239..2789)
                     /locus_tag="VFX21_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX5094722.1"
                     /translation="MWPGLHAKERPEHIAYVMAGTDRAVTYRELDDRSNQVAQMLFDA
                     GLRFGDHIAVLMENRPEYFEICWAAQRSGLFYTCINWHFTAEEAAYIIDDCDARVLFV
                     SEAYRDLADELRDLMPHVELRVMVGGTTDGYIDYAEARDRFPAEPLAEELEGTPMLYS
                     SGTTGRPKGIKYRIAREPVGAMPSNMGMLTAVFGMTTESIYLSPAPLYHSAPLFYCMS
                     TMRLGGTVVVMENFDPEEALRLIEKHGITHSQWVPTMFVRMLKLPTDVRTRYDLSSHK
                     CAIHAAAPCPVEIKQQMIDWWGPIIEEYYSATEGMGATYINSADWLTHRGSVGKSLIS
                     AIHIVDEAGNDMPPGEVGTVYFEPPANRPGFEYHKDEAKTKEVFDTKGRASVGDMGYL
                     DDEGYLYLTDRRTFMIVSGGVNIYPQEAENALITHPKVFDVAVFGIPDAEMGEKVHAV
                     VQPADWDDAGPELAQELLAYCREQLSHYKCPKAIDFDKELPRQPTGKLYKRLLRDRYW
                     GNKTSRIV"
     gene            complement(2796..3878)
                     /locus_tag="VFX21_01855"
     CDS             complement(2796..3878)
                     /locus_tag="VFX21_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018637247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEX5094723.1"
                     /translation="MKAAMFVETGAPLTIENVEPAPPGPRDVVVEVGASGVCHSDLSL
                     KNGYVGIMPGTILGHEGAGKVVEVGNEVTKVKPGDRIIASFIPACGVCHFCLHDQSHL
                     CDNEMNVMMSMRGARPDGSQYMAMTGLGTFAEAMTCNEGSIVKVDTDLPDEQLALIGC
                     GVTTGVGAALNTAGVHPGATVAVIGCGGVGQAVIQGARIAGASRIFAIDMVDLKRKTA
                     EQLGATDLIDPNDGDPIAQIQNATGGRGVDYAFEVIGLPETALQAYMMIRKGGTAIMV
                     GMTRAEATLTIPTFDLFFNEKTLKGSKYGSGQVRRDFQRFIDLIETGRLDTSSMVSRT
                     IGLEDVNEAFRAMEAGEVIRSVITTF"
     gene            4030..4470
                     /locus_tag="VFX21_01860"
     CDS             4030..4470
                     /locus_tag="VFX21_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX5094724.1"
                     /translation="MSDQNAAIPRRHVVIVTCMDARVDVLGAFDFAPGDAHLLRNAGA
                     VITDDVLRSIVLSQRLLQTRSVLIVGHTECGLRTFTDAELTKALAWETGQKPPYAWGA
                     FLDLEEHVRTSVQRVRDCPWLVHADDVHGYVYDVNDHTVRLLTP"
BASE COUNT          674 a         1532 c         1559 g          821 t
ORIGIN      
        1 cgggctccac cagcgtctcg cgaactgctt gccgacgagt ggccgtaggg gttgcagcac
       61 cgtgccgacg atcttcgggc ccggcacccg cgggcacgtc agcaacgcgt aggtcagcgc
      121 ggcgtcgctc atcccttcgc cacgtgccgc gttgccccag tcgatgacga ccggaccggt
      181 cgccgccatg atgacattca acgggtgcag atcgaggtgc acgacgcgat cgccgccgtc
      241 gggcatctgc cggaggtggt cgggcgccgc gatctcgtgc agctggtcgt gcaggtccgc
      301 gagcacgcgc agcatcgcgg gcatcttcca cggcttgagc atcgtgtcca tcatcagtgg
      361 accgtcgatg cggtccatca cgatctccga cccgttggcg cgcacgtcgt ggatcttcgg
      421 cacgggatag ccgtgcgcgg ccacgtaccg gatgatgcgc gcttcgtgct cgatcgagcg
      481 gccgtcgcgc gtcttgcgca gcacgagccc gggaccgaac tcgaagacgt cgccgtcgcg
      541 gcccgacgcg aggatggggc cgggctcggt cacgttgcga acatacgcca cctgcttgac
      601 tggagcccgt gcgagatggg ttgccccgac tggtgctcgt cgacgggctg ccgggttccg
      661 gcaagacgac gaccgcggaa cgcatgtgcc gccggctgcg ctcgcggggc atcgacgccg
      721 cgtgggctgc tgagactgcg cccgatcatc ctgtgttccc gcgcggcttc gcgaagtcgc
      781 gacgcgagcc cgagttcccg cagcagtgcc tcgacgcgtg ggcggcattc gtcgacacga
      841 tcgggacgcg cacgtgggtg ctcgacggtg ccgcgttcca gtcgacggtg cggttcttct
      901 acgacgagcg cgtgccctac gacgagatct tcgcctactg gcgcgcgttc gagacgatcg
      961 tcatgcccct ccggccgacc atcttctacc tgcgcgacga caatcccgcc gatcggatgc
     1021 gaacgatcac gatcccggtg cgcgggcgag cctggtacga caaggtctcc aactacgtcg
     1081 cgcggacgcc ggccggacgt gagttccgcc atctcggtgt cgaaggcttc atcgcgttct
     1141 gggtccgcta cggcgagctg tgcgagtcgc tgcttgcgca aacgcggctc gacgtcgcat
     1201 cttctccgct cggcctggcg ccggagccgg cgctggtctc agacgatgcg tgaggtcttg
     1261 ttgccccagt agcggtcgcg cagcagccgc ttgtacagct tgccggtcgg ctggcgcggc
     1321 agttccttgt cgaagtcgat cgccttcggg cacttgtagt gcgagagctg ttcgcggcag
     1381 taggcgagca gctcttgtgc cagctccggt cccgcgtcgt cccagtcggc gggctgcacg
     1441 accgcgtgga ccttctcgcc catctcggcg tcggggatgc cgaacacggc gacgtcgaag
     1501 accttcgggt gcgtgatgag cgcgttctcc gcttcttgcg ggtagatgtt gacgccgccc
     1561 gacacgatca tgaacgtgcg ccggtcggtg aggtagaggt agccctcgtc gtcgaggtag
     1621 cccatgtcgc cgacgctggc ccggcctttc gtgtcgaaga cttccttcgt cttcgcctcg
     1681 tccttgtggt actcgaagcc gggccggttg gccggcggct cgaagtagac ggtgccgact
     1741 tctcccggcg gcatgtcgtt gcccgcttcg tcgacgatgt ggatcgcgga gatcagcgac
     1801 ttgccgaccg agcctcggtg cgtgagccaa tcggcgctgt tgatgtacgt cgcgcccatg
     1861 ccttcggtcg cggagtagta ctcctcgatg atcgggcccc accagtcgat catctgctgc
     1921 ttgatctcga cggggcacgg cgcggcggcg tggatcgcgc acttgtgcga cgacaggtcg
     1981 tagcgcgtgc gcacgtcggt gggcagcttc agcatgcgca cgaacatcgt cggcacccac
     2041 tggctgtgcg tgatgccgtg cttctcgatc aggcgcaacg cttcctcggg atcgaagttc
     2101 tccatgacca cgacggtgcc gccgaggcgc atggtgctca tgcaatagaa caacggcgcc
     2161 gagtggtaca gcggcgcggg cgacaggtag atcgactcgg tcgtcatgcc gaacaccgcg
     2221 gtgagcatgc ccatgttcga cggcatcgca ccgacgggtt cacgcgcgat gcggtacttg
     2281 atgcccttgg gtcgtccggt cgtgccggac gagtacagca tcggcgtgcc ttcgagctct
     2341 tcggccaacg gttccgcggg gaagcggtcg cgcgcctcgg cgtagtcgat gtacccgtcg
     2401 gtggtaccgc cgaccatcac gcgtagctcg acgtgtggca tcaggtcgcg cagctcgtcc
     2461 gcgaggtcac ggtaggcctc gctgacgaac agcacgcgcg catcgcagtc gtcgatgatg
     2521 tacgcggctt cttcggcggt gaagtgccag ttgatgcacg tgtagaacag ccccgagcgc
     2581 tgggcggccc agcagatctc gaagtactcg gggcggttct ccatgagcac cgcgatgtgg
     2641 tcgccgaagc gcaggcccgc gtcgaagagc atctgcgcga cctggttcga gcggtcgtcg
     2701 agctcgcgat acgtgacggc gcggtcggtc cccgccatca cgtacgcaat gtgctcaggg
     2761 cgctccttgg cgtggagccc gggccacacc tcggcttaga aggtggtgat gaccgagcga
     2821 atgacttcgc cggcctccat cgcccggaat gcttcgttga cgtcctcgag cccgatcgtg
     2881 cgtgacacca tcgacgaggt gtcgaggcgg ccggtctcga tgaggtcgat gaagcgctgg
     2941 aagtcgcggc gcacctgacc cgacccgtac ttcgaaccct tcagcgtctt ctcgttgaag
     3001 aagaggtcga acgtcgggat cgtgagcgtc gcctccgcac gtgtcatgcc gaccatgatc
     3061 gcggtgccgc ccttgcggat catcatgtac gcctgcagcg cggtctcggg gagaccgatc
     3121 acctcgaacg catagtcgac cccgcggcct cccgttgcgt tctggatctg cgcgatcggg
     3181 tcgccgtcgt tggggtcgat gaggtcggtc gcgccgagct gctccgcggt cttgcgcttc
     3241 aagtcgacca tgtcgatcgc gaagatgcgc gacgcgcccg cgatgcgcgc gccctggatg
     3301 acggcctgac cgacaccgcc gcagccgatg accgcgacgg tcgcaccggg gtggaccccc
     3361 gccgtgttca gcgccgcgcc gacacccgtc gtgacaccgc aaccgatcaa cgcgagctgt
     3421 tcgtcgggaa ggtcggtgtc gaccttgacg atcgagccct cgttgcacgt catcgcttcc
     3481 gcgaacgtgc cgaggcccgt catcgccatg tactggctgc cgtcggggcg ggcgccgcgc
     3541 atcgacatca tgacgttcat ctcgttgtcg cacaggtgcg actggtcgtg caggcagaag
     3601 tgacacacgc cgcacgcggg gatgaaggag gcgatgatgc ggtcaccggg cttcaccttg
     3661 gtgacctcgt tgccgacctc gacgaccttg cccgcgccct cgtggccgag gatcgtgccg
     3721 ggcatgatgc cgacgtagcc gttcttgagc gacaggtcgg agtggcagac gcccgatgca
     3781 cccacttcga ccacgacgtc gcgcggcccg ggcggcgccg gctcgacgtt ctcgatcgtg
     3841 agcggggcgc ccgtttcgac gaacattgcg gccttcatac gtgcctccca agagcgacgg
     3901 tcgacgtgct ggtcgaggtg atcgggacga taccgcgccg ggccgcgagc ggtcacggcc
     3961 gtacgggcgg gccgaccggt cccgcttcga ggtgcgagcc ggcacggcgc cgggcgaagc
     4021 tgtggcctga tgtccgacca gaacgccgcg atcccgcgcc gccacgtcgt gatcgtcacg
     4081 tgcatggatg cgcgtgtcga cgtgctcggc gcgttcgact ttgcgccggg cgacgcgcac
     4141 ctgctgcgca acgccggcgc agtcatcacc gacgacgtgc tccgttccat cgtgctgtcc
     4201 caacgactct tgcagacccg ttcggtgctg atcgtcggtc acaccgagtg cgggctgcgc
     4261 acgttcaccg acgcggagct cacgaaggcg ttggcgtggg agaccggaca gaagccgccg
     4321 tacgcgtggg gcgcgttcct cgacctcgag gagcacgtgc gcacgtcggt ccagcgggtg
     4381 cgcgactgcc cgtggttggt gcacgccgac gacgtccacg gctacgtcta cgacgtgaac
     4441 gaccacaccg tgcggctcct cacgccgtag actttctcgg tgcgcacgct cgccttcttc
     4501 ggcccggccg gcacgttcgc cgaggaggcg ctgctgaccc aaggcgatct ggccgcgtgc
     4561 gagcgcaccc cgttccggtc cgtccc
//
LOCUS       DASUGA010000100         2814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1723170, whole genome shotgun sequence.
ACCESSION   DASUGA010000100 DASUGA010000000
VERSION     DASUGA010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1723170"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1558
                     /locus_tag="VFX21_01865"
     CDS             131..1558
                     /locus_tag="VFX21_01865"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018500735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5094725.1"
                     /translation="MRHDHWIGGKADAPASGEYLSTIDPATRAPGDEIAAGTAADVER
                     AVAAAAAAQPDWADRSAAERADVLRHVADAVDADADELIDLERACTGKIAAQLRMEVD
                     MSVAYLRYYAGVLRAHHGRVIEQGAGVHTYTRLEPYGVVAVITPWNLPLNQACRAASP
                     ALAVGNAVVAKPSEFTSTSTLHLARLATEAGLPDGVFNVVTGTGPDAGTPLVSHPAVR
                     RIAFTGSVATGRLLAGLAAERLVPMTVELGGKSPIVVFADADLDRAVAAAVATVAMNS
                     GQVCSATTRLLVEASVHDEVAGRVVEAVARLQPGVDFGPMITEAQYAKVLGHFEAARG
                     AGAVPATGGAGYDDGPGAAGLYVQPTVYAGVDPELRVAREEVFGPVLVTMPFADEADA
                     IARGNDTEYGLVASVWSGDLARGLRVAERLDAGQVAVNGGPLTIETPFGGFKGSGYGR
                     EKGLEALHEYAQVKTISASLAPKRD"
     gene            1580..1972
                     /locus_tag="VFX21_01870"
     CDS             1580..1972
                     /locus_tag="VFX21_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5094726.1"
                     /translation="MDDSEQLRAAQAMRRAMLGDEYVDSQGADPDPITAEFQDYVTSM
                     AWGVWAREGALSPRDRSLLVMAMTAALGRMEEFKLHAGAQARTGVTDAEIDELLFQIA
                     AYCGAPAGISARRQIKAVRAEREAGPRS"
     gene            1969..>2814
                     /locus_tag="VFX21_01875"
     CDS             1969..>2814
                     /locus_tag="VFX21_01875"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018500737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5094727.1"
                     /translation="MSDLVVGFVGLGNMGSVLAANLVTAGLTVVAHDAVGPARAPDGA
                     EYVESVGDVARAADVVVFSLPDGAVSELVAREVVAADDRRVTHVVDTSTVGPHAAQAI
                     AALLADADVAYVDAPVSGGIAGARARTLAVMVSADDAACAYVEPVLAGLSKRRHRVGD
                     RPGLGQALKLANNFLSATALAATSEAVAFGTALGLDMATMVEVLNASSGNNTATSDKF
                     PNHVITGRYSSGFANSLMAKDVRLYLAEVREQGGPSTVGMVTAEIWEQFAAADPGVDF
                     TRIYPY"
BASE COUNT          412 a          967 c         1046 g          389 t
ORIGIN      
        1 gggtgacccc accaacgaca tcacgaccat cagcaccgtc cccttcgtga tgaaggacgg
       61 tgtggtctac aagcacccct gatgtcgccg cacgcgacga ggtaacccgg gcccgagagg
      121 aaggcgatcg gtgcggcacg accactggat cggcggcaag gccgacgcgc cggcgagcgg
      181 cgagtacctc tcgacgatcg atcccgcgac gcgcgcgccg ggcgacgaga tcgcggcggg
      241 cactgcggcc gacgtcgaac gcgcggtcgc ggcggccgcg gccgcgcaac cggattgggc
      301 agatcggtcg gctgcggagc gggccgacgt gctccggcac gtggccgatg ccgtcgacgc
      361 cgacgccgat gagctcatcg acctcgagcg cgcgtgcacg ggcaagatcg ccgcgcagct
      421 gcgcatggaa gtcgacatgt cggtcgcgta cctgcgctac tacgcgggcg tgctccgcgc
      481 gcaccacggc cgcgtcatcg aacagggcgc cggcgtgcac acgtacacgc ggctcgagcc
      541 gtacggcgtc gtggccgtga tcacgccgtg gaacctgccg ctcaatcaag cgtgccgcgc
      601 cgcgtcgcct gcgctcgcgg tggggaacgc cgtggtggcg aagccgtcgg agttcacgtc
      661 gacgtcgacg ctgcacctcg cccgactcgc gaccgaggcc ggtctcccgg acggcgtgtt
      721 caacgtcgtc acgggtaccg gcccagacgc gggaaccccg ctcgtgtcgc atccggccgt
      781 gcggcggatc gcgttcaccg gatcggtcgc gaccggtcga ctcctggccg ggctcgcggc
      841 cgaacggctg gtgccgatga cggtggagct cgggggcaag tcgccgatcg tggtgttcgc
      901 cgacgccgat ctcgatcgcg ccgtcgccgc ggcggtcgcc acggtggcca tgaactcggg
      961 gcaggtgtgc tccgcgacga cccggctgct ggtcgaggcg tcggtgcacg acgaggtggc
     1021 gggccgcgtg gtcgaggcgg tcgcccggct ccaaccgggg gtcgacttcg gtcccatgat
     1081 caccgaagcg cagtacgcga aggtgctcgg gcacttcgag gccgctcgcg gcgcgggcgc
     1141 agttcccgcg acgggcggag ccgggtacga cgacggtccc ggcgcggccg gcctgtacgt
     1201 acagccgacg gtgtacgccg gcgtcgatcc ggagctgcgc gtcgcgcgcg aagaggtgtt
     1261 cgggcccgtc ctcgtgacga tgccgttcgc cgacgaagcc gacgcgatcg cgcgcggcaa
     1321 cgacaccgag tacggcctcg tcgcgagcgt ctggagcggc gatctcgcgc gcgggctgcg
     1381 cgtcgccgag cggctcgacg ccggccaggt cgccgtgaac ggcggaccac tcacgatcga
     1441 gacgccgttc ggcggcttca agggcagcgg ctacgggcgc gagaaggggc tcgaggcgct
     1501 gcacgagtac gcgcaggtga agacgatcag cgcatcgctt gccccgaaac gcgactgaac
     1561 agaaggcggg agaacgttga tggacgattc cgaacagctg cgcgcggcgc aagcgatgcg
     1621 gcgggccatg ctcggggacg agtacgtcga ctcgcaaggc gccgatcccg atcccatcac
     1681 cgccgagttc caggactacg tcacatcgat ggcgtggggc gtctgggcgc gcgagggcgc
     1741 gctgtcgccg cgtgatcgca gcctcttggt gatggcgatg accgccgcgc tcggtcgcat
     1801 ggaggagttc aagctgcacg cgggcgcgca ggcccgcacc ggtgtgaccg acgccgagat
     1861 cgacgagctg ctgttccaga tcgcggcgta ctgcggtgct ccggcgggga tctcggcccg
     1921 ccgtcagatc aaggcggtgc gcgccgagcg cgaagcaggg ccgcgatcgt gagcgacctc
     1981 gtcgtcgggt tcgtcgggct cggcaacatg gggagcgtgc tcgcggcgaa cctcgtgacg
     2041 gccgggctca ccgtggtcgc gcacgacgcc gtcggtccgg cgcgcgcacc ggacggcgcg
     2101 gagtacgtgg agtcggtggg cgacgtggcg cgcgccgcgg acgtcgtcgt cttcagcctt
     2161 cccgatggcg cggtgtccga gctggtggcg cgtgaggtcg tggccgccga cgatcgccgg
     2221 gtcacacacg ttgtcgacac ctccacggtc ggaccccacg cggcgcaggc gatcgccgcg
     2281 ctcctcgccg atgcggacgt cgcgtatgtc gacgcgccgg tgtcgggtgg catcgccggt
     2341 gcgcgggcgc gcacgctcgc ggtcatggtg tcggccgacg atgccgcgtg cgcgtacgtc
     2401 gagccggtgt tggccgggct cagcaaacgt cgtcaccgag tcggagaccg cccggggctc
     2461 gggcaggcgc tcaagctcgc caacaacttc ctgtcggcca ccgcgctcgc ggccacgagc
     2521 gaggccgttg ccttcggtac cgcgctcggc ctcgacatgg cgaccatggt cgaggtgctg
     2581 aacgcgtcga gcggcaacaa caccgcgacg agcgacaagt tccccaacca cgtgatcacg
     2641 ggtcgctact cgtcgggatt tgccaactcg ctcatggcca aggacgtacg gctctacctc
     2701 gccgaggtcc gggagcaggg cggcccgtcc acggtcggca tggtcacggc cgagatctgg
     2761 gagcagttcg cggccgccga ccccggcgtc gacttcaccc gcatctaccc gtac
//
LOCUS       DASUGA010000101         3492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_961439, whole genome shotgun sequence.
ACCESSION   DASUGA010000101 DASUGA010000000
VERSION     DASUGA010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961439"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFX21_01880"
     CDS             <1..213
                     /locus_tag="VFX21_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094728.1"
                     /translation="VTLTGAVYAFGDAQYYGNAPPSADSFTVGLSPTPAGYVFVDTKG
                     RVTNRGGAHVPSSLGPSFSHFVGVAS"
     gene            complement(230..673)
                     /locus_tag="VFX21_01885"
     CDS             complement(230..673)
                     /locus_tag="VFX21_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094729.1"
                     /translation="MQLRRPKHPGRVAVVTVGSLLAVNLLVFAALAQDTSTARDKRPE
                     SIEQLEPGEDAQAPPQATIIVDLADNLQAMLYINDTLVPEDEYEGDVTLGQLIYRPGD
                     GKTFEELPEGTNNFAIEYWARTKSYDDALAAGEVLAYSWQVKVGF"
     gene            731..2587
                     /locus_tag="VFX21_01890"
     CDS             731..2587
                     /locus_tag="VFX21_01890"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX5094730.1"
                     /translation="MPRSVVHDLAGRVLAGRYLLQSPIGTGASGRVYAAEDTRLRRRV
                     AVKVLHAALADDAAFLRRFRAEAQVAASLHHPHIVTVHDWGEDDVPFMVQELLEGGSL
                     RSMLDAGNHLSVAQAARVGRDVASALDYAHARGILHRDIKPANLLFDEHGIVRVADFG
                     LARALAEASWTEPSDAVFGTARYASPEQAMGAQLDARSDLYALSLVIVEAVTGRVPFT
                     ADTTIGMLTARTQKAMLAPEELGPLVPVLERAGRIDPDERYPDAATMRSALNDVAESL
                     PPPAPLTLAGMNDADDPHPTRMQQPETARTMLFDQDTAEITVEETPAAKRRERRLVPF
                     VVAAVLVLAVAFAAAAISQMGGSSIEVPGLVGLTVDDAKAAATDADLSLTVAGEQHAP
                     DPAGTVISQDPEAGSFTGGDDVSVVVSSGPPPVAVPTVVGQAWDQANAALVGAGFTVD
                     RQDQFFDDIPADNVASVDPAAGAEIAPGSKVTVVVSKGHAPVAVPPVVGKTFEEAQSA
                     LTGASFTVKRADEFSDDVESGKVIESTPAEGGEAPFGSEVTLAVSKGPELVAVPDVRN
                     LKLDDAASALEDVGLQVAVNGPYRPGKKVVTQTPSPGTQVRKGETVSVAVEN"
     gene            2615..>3492
                     /locus_tag="VFX21_01895"
     CDS             2615..>3492
                     /locus_tag="VFX21_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005243383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5094731.1"
                     /translation="MGSLDGRVAIITGAGRGLGREHALLFASEGAKVVVNDLGGDMHG
                     EGGDPSAAMQTVDDIKAMGGEAVVNGDNVADWDGAARLVQQAIDAFGDLHILVNNAGI
                     LRDRVIINMTEAEWDAVVNVHLKGHFCPTRHAATYWREQTKAGKTVDAAIVHTTSTSG
                     LFSNPGQANYDAAKSGIATFSQVCAKELSRYGVRSNSIAPAARTRLTEATPGLGDAVQ
                     APAEGFDKWDPANVAPFVAYLSTAECPFTGETFFVQGGKIQRVQSWAFAETIDKGDRW
                     TVAELAAEGKRLAPSG"
BASE COUNT          522 a         1213 c         1218 g          539 t
ORIGIN      
        1 gtgacgctga ccggtgcggt ctacgcgttc ggtgacgcgc agtactacgg caatgcgccg
       61 ccgtcggcgg actcgttcac cgtcggtctc tcgccgacgc cggccggcta cgtcttcgtc
      121 gacacgaagg gccgggtcac gaatcgcggc ggcgcccacg tgccgtcgtc actcggcccg
      181 agcttctccc acttcgtcgg cgtcgcgtcc taggactcgg cacgcccgcc tagaacccga
      241 ccttgacctg ccaggagtac gcgagcactt cgccggcggc gagcgcgtcg tcgtaactct
      301 tcgtgcgcgc ccagtactcg atcgcgaagt tgttcgtgcc ttcggggagc tcttcgaacg
      361 tcttgccgtc gcccgggcgg tagatcagtt gtccgagcgt gacgtcgccc tcgtattcgt
      421 cttcggggac gagcgtgtcg ttgatgtaga gcatcgcctg caagttgtcg gcgaggtcca
      481 cgatgatcgt cgcctgtggc ggcgcttgcg cgtcctcgcc gggttcgagt tgctcgatcg
      541 attccggacg cttgtcgcgc gcggtactgg tgtcctgcgc gagtgcggca aagacgagga
      601 gattgaccgc gagcaacgat ccgacggtca cgacggcgac tcgtccgggg tgcttcggcc
      661 ggcgtagctg cacagagtga cgctaccggg ctgccgcggt tttccggcat ccggccggta
      721 cggttgtccc atgccgcgga gtgtcgtgca tgacctcgcg ggccgagttc tggccggccg
      781 atacctcctc cagtctccga tcgggactgg cgcgagcggt cgcgtgtacg cggccgagga
      841 cacccgtctc cggcgccggg tggcggtcaa ggtcttgcac gccgcgctcg ccgacgacgc
      901 cgcgttcctc cgccggttcc gggccgaggc ccaggtggcg gcgtcgttgc accacccgca
      961 catcgtgacg gtgcacgact ggggcgagga cgacgtgccg ttcatggtcc aggagttgct
     1021 cgaaggcggc agcttgcgct cgatgctcga cgccggcaat cacctgtcgg tcgcgcaggc
     1081 cgcacgcgtc gggcgcgacg tcgcgtcggc gctcgactac gcgcacgcgc gcgggatcct
     1141 gcaccgcgac atcaagcccg cgaacttgct cttcgacgag cacggcatcg tgcgcgtcgc
     1201 cgacttcggt ctggcgcgcg cgctcgccga ggcgagctgg accgaaccat ccgacgcggt
     1261 cttcggcacc gcacggtacg cgtcgccgga gcaggcgatg ggcgcgcaac tcgatgcgcg
     1321 ttccgatctc tatgcgttgt cgctcgtgat cgtcgaggcg gtcaccggcc gcgtgccgtt
     1381 cacggccgac acgaccatcg gcatgctcac cgcgcgcacg cagaaggcga tgctcgcgcc
     1441 cgaggagctg gggccgctcg ttcccgtgct cgaacgcgcc ggccgtatcg accccgacga
     1501 gcgctacccc gacgcggcga cgatgcgttc cgcattgaac gacgtcgcgg agtcgctgcc
     1561 gccgcccgcg ccgttgacgc tcgcggggat gaacgacgcc gacgatccgc atccgacacg
     1621 catgcagcag ccggagacgg cgcgcacgat gctgttcgac caggacactg ccgagatcac
     1681 ggtcgaagag acgccggccg cgaagcggcg cgagcggcgg ctggtgccgt tcgtcgtggc
     1741 cgccgtgctc gtcctggcgg tcgcgttcgc ggccgcggcc atctcgcaga tgggtggctc
     1801 gtccatcgaa gtacccggcc tcgtcgggct cacggtcgac gacgcgaagg cggccgcgac
     1861 cgacgccgac ctgtcgctca ccgtcgccgg cgagcagcac gcgcccgatc cggccggcac
     1921 cgtgatcagc caggatcccg aggccggttc gttcacgggt ggcgacgacg tctcggtcgt
     1981 cgtgtcttcc ggaccgccgc ccgtcgcggt cccgaccgtc gtcggccagg cctgggacca
     2041 ggcgaatgcc gcgctcgtcg gcgcgggctt caccgtcgac cgccaagacc agttcttcga
     2101 cgacatcccg gccgacaacg tcgcgtccgt cgatccggcc gcgggcgccg agatcgcgcc
     2161 gggatcgaag gtgaccgtcg tcgtgagcaa gggccacgcg ccggtcgcgg tgccgccggt
     2221 ggtcggcaag acgttcgaag aggcgcagag cgcgctcacc ggcgcgagct tcaccgtgaa
     2281 gcgcgccgac gagttctccg acgacgtcga gtcgggcaag gtcatcgagt ccacgcccgc
     2341 cgaaggcggg gaggcgccgt tcggttccga ggtcacgctc gccgtgagca agggtcccga
     2401 gctcgtcgcg gtccccgacg tgcgcaatct gaaactcgac gacgccgcct cggccctcga
     2461 ggacgtcggc ctccaggtcg cggtcaacgg gccgtaccgc ccgggcaaga aggtcgtgac
     2521 ccagacaccg tcgcccggca cgcaggtgag gaagggcgag accgtctccg tcgcagtgga
     2581 gaactgatct ttctcacaca tacgctctca cttcatggga tcacttgacg gacgcgtggc
     2641 gatcatcacc ggtgccggtc gcggcttggg gcgcgagcac gcactgctgt tcgcgagcga
     2701 aggtgcaaag gtcgtcgtca acgacctggg cggcgacatg cacggcgagg gcggtgaccc
     2761 gagtgccgcg atgcaaaccg tcgacgacat caaggcgatg ggcggagaag ccgtcgtcaa
     2821 cggcgacaac gtcgccgact gggacggcgc cgcccgtctc gtgcagcaag cgatcgacgc
     2881 gttcggtgac ttgcacatcc tcgtgaacaa cgccggcatc ctgcgcgacc gcgtgatcat
     2941 caacatgacc gaggccgagt gggacgccgt ggtcaacgtg catctgaagg gtcacttctg
     3001 cccgacgcgc cacgccgcca cgtactggcg cgagcagacc aaggccggca agacggtgga
     3061 cgcggcgatc gtgcacacga cgtcgacgtc gggtctgttc tcgaacccgg gccaggcgaa
     3121 ctacgacgcc gcgaaatccg ggatcgcgac gttctcgcag gtctgcgcga aggagctgtc
     3181 gcgctacggc gtccgctcga actcgatcgc gcccgcggcc cgcactcggc tcaccgaggc
     3241 gacgccgggc ctcggcgacg cggtgcaggc gccggccgag ggcttcgaca aatgggatcc
     3301 ggccaacgtg gcgccgttcg tcgcctacct gtcgacggcc gagtgcccgt tcaccggcga
     3361 gacgttcttc gtccagggcg gcaagatcca gcgcgtgcag tcgtgggcct tcgcggagac
     3421 catcgacaag ggcgaccgct ggacggtggc cgagctcgcc gccgagggca agcggctcgc
     3481 cccgagcggg ta
//
LOCUS       DASUGA010000102         2096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1028247, whole genome shotgun sequence.
ACCESSION   DASUGA010000102 DASUGA010000000
VERSION     DASUGA010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028247"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VFX21_01900"
     CDS             complement(<1..622)
                     /locus_tag="VFX21_01900"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX5094732.1"
                     /translation="MAIDVRELATRTLDPVITEELDIFENTVSEYLDGRVDEDAFRVF
                     RLNHGVYGQRQGGTNQMLRVKIPHGRLTPEQLDTFAYIADTYSRGWGHITTRQNMQFH
                     FIQLEQSPEILRLLAAAGMTSREACGDTVRNVMGCHLAGACPQEKFDISPWAQATTDF
                     LLRHPYAQRLPRKFKINFSGCATDCGQAMFNDVGVVASTRTLPDGTE"
     gene            complement(895..1707)
                     /locus_tag="VFX21_01905"
     CDS             complement(895..1707)
                     /locus_tag="VFX21_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094733.1"
                     /translation="MSNEFDDIDDLDAPWRDHRDELLERVTRRGKALRVRRQVVLVSV
                     VSAILVMPFAAVAATNTQTDVPVLRWASRVSSESAASTSTTTSTIATVSSTTANTTVP
                     GRTVLTQPVLPGTAATTTTVTACRNSVDPACGQFRWDPAPPAVNAIPIGVLAVNGPPG
                     ADVAIGIDLDVPALLDIDYGDGTTETRLVAPAPCPGTPAGPWSWPTLAGAEVAPLQHT
                     YAAEGSYSVTVIARPVEFCGHASPYTDGVAGGIATISLEPPTTTTTTTPAGP"
     gene            complement(1704..>2096)
                     /locus_tag="VFX21_01910"
     CDS             complement(1704..>2096)
                     /locus_tag="VFX21_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5094734.1"
                     /translation="EDLAAEALARTYARWGRVRTMDYREAWVARIATNLAIDAARKKK
                     VDLAAAVEADPSDGTVLRIALVDALARLPRRQREAVALRYLCDLSVDDVAHSLGVSTG
                     TVKTSVHRALEKLRNDLGSDAALIGEEQ"
BASE COUNT          318 a          653 c          766 g          359 t
ORIGIN      
        1 cctcggtgcc atcgggcaat gtacgcgtgc tcgcgacgac gccgacgtcg ttgaacatcg
       61 cctggccgca atcggtcgcg cagcccgaga agttgatctt gaacttgcgc ggcagccgct
      121 gcgcgtacgg gtggcgcaac aagaagtcgg tcgtggcctg cgcccagggg gagatgtcga
      181 acttctcttg cgggcacgcg ccggcgaggt ggcagcccat gacgttgcgg acggtgtcgc
      241 cgcacgcttc tcgcgacgtc atcccggccg cagcgagcag gcgcaggatc tcgggcgact
      301 gctcgagctg gatgaagtgg aactgcatgt tctggcgggt cgtgatgtga ccccagcccc
      361 gcgagtacgt gtcggcgatg tacgcgaacg tgtcgagctg ctcgggcgtg agccggccgt
      421 gcgggatctt gacccgcagc atctggttgg tgccgccctg gcgctggccg tagacgccgt
      481 ggttcaaccg gaagacgcgg aacgcgtcct cgtcgacgcg accgtcgagg tattcgctga
      541 cggtgttctc gaagatgtcg agttcttcgg tgatgaccgg gtcgagcgtg cgagttgcga
      601 gttcgcgaac gtcaatcgcc atgcggcggc tccctggtgg ggtggctgga gaagaagacg
      661 gcgggcggcg gagaggccgg ggccgtcagc gggcggacgc gcgacagagt tcgtcgtgca
      721 tctcgtgcgg cgtgcgtccc atgaagatgg atgcggcgcc gtgatcccgc agaatcatgg
      781 ccacaaatct acatcggccg gcacctaaat accactaaga aggtcaggaa tatttccgac
      841 cgtttgggtc agaaatgtgc accgatctgg tggagatccc aaatccggtg gagatcaagg
      901 gccggcggga gtcgtggtgg tcgtcgtcgt gggcggctcg agcgaaatcg tcgcgattcc
      961 gcctgccacg ccgtccgtgt acggcgatgc atgtccgcag aattcgaccg gccgggcgat
     1021 cacggtgacc gaatagctcc cttcggccgc ataggtgtgt tggagcggcg cgacctcggc
     1081 tcccgcgagc gtcggccacg accacggccc ggccggtgtg cccgggcacg gtgccggcgc
     1141 cacgaggcgc gtttcggtcg tgccgtcgcc gtaatcgatg tcgaggagcg cgggaacgtc
     1201 caggtcgatg ccgatcgcga cgtcggcgcc gggcgggccg ttgacggcga ggacaccgat
     1261 cggaatcgcg ttcaccgccg gcggcgcggg gtcccagcgg aactgaccgc acgcggggtc
     1321 gacgctgttg cggcacgcag tcaccgtggt tgtcgtcgcg gccgttcccg gcaagacggg
     1381 ttgcgtcaac accgttcgcc ccggaaccgt cgtgttcgca gtcgtcgaac tcacggttgc
     1441 gatcgtcgac gttgtggtcg acgtcgacgc ggccgactcg gaactgacgc ggctggccca
     1501 gcgcaacacc ggcacgtcgg tctgggtgtt cgtcgcggcg accgccgcga acggcatgac
     1561 caggatcgca ctgaccacgc tgaccagcac gacttggcgg cggacgcgca gcgccttccc
     1621 gcggcgcgtg acgcgttcca acagctcgtc gcggtgatcg cgccagggag cgtcgaggtc
     1681 gtcgatgtcg tcgaattcgt tactcattgt tcttcaccga taagcgcggc atcggagccg
     1741 aggtcgttgc ggagtttctc gagcgcgcgg tgcactgacg tcttgacggt tccggtgctg
     1801 acgccgagcg agtgcgcgac gtcgtcgacg ctgaggtcgc agaggtagcg cagtgcgacg
     1861 gcttcccgct ggcggcgggg gagccgcgcc agcgcgtcga caagtgcgat gcgcagcacc
     1921 gtgccgtcgg acgggtcggc ctcgaccgca gcggcgaggt ccaccttctt cttgcgggcc
     1981 gcgtcgatgg cgaggttggt cgcgatgcgc gccacccagg cttcgcggta gtccatggtc
     2041 cgcacgcgtc cccagcgggc gtaggttcgg gcgagcgcct cggccgcgag gtcctc
//
LOCUS       DASUGA010000103         3465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_54221, whole genome shotgun sequence.
ACCESSION   DASUGA010000103 DASUGA010000000
VERSION     DASUGA010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54221"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VFX21_01915"
     CDS             <1..299
                     /locus_tag="VFX21_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094735.1"
                     /translation="TFDEARIEHQDAVTRYGAHGVPTIVFESGYAVYGPVVVPAPTGE
                     DALALWELVAGWQRFPHLYELRHPKTHHDLVHVAEQFNTYLTTRDWNTVENPAP"
     gene            complement(423..2843)
                     /gene="gyrA"
                     /locus_tag="VFX21_01920"
     CDS             complement(423..2843)
                     /gene="gyrA"
                     /locus_tag="VFX21_01920"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX5094736.1"
                     /translation="MARKKAASTQLTLEAADVPSRTLVAEVESAFLEYSMSVIVARAL
                     PDVRDGLKPVQRRILWAMHDANLRPDRPFVKCARVVGDVMANYHPHGDSSIYEALVRM
                     GQPFSLTGPLIDKHGNFGSPSDPPAAARYTECRLSQLAMEMLAGIDEGTVDFEPNYDA
                     STTEPLVLPSRFPNLLVNGSQGIAVGMATNIPPHNLAEVCNAALKVIDQPDITLPALM
                     RVIKGPDFPTGGMILGDDGIKDAFRTGRGTIRVRATHEIEDVPRRGQAIVLTEVPFQT
                     SVDAIAGKLAELVENGKLDGVRDIRNESGQGKTRLVIELRPDANPQIVLNNMFKHTTA
                     QTTFPVNMVALVDGVPRTVSLIDALHAWVDHQVIVVTRRTQHRLEKAEARLHIVEGLV
                     RALDMIDEIVKAIRASKDRAAARTKLMAKPFDFSEIQANHILDMALGRLTQLGREELA
                     NEKEELEETIKGLKRILAKKDVLMGVIKDELTVIRDAHKAPRRTRIEADETGTIDVVA
                     LVEDEPYVVTVTARGYIRAVPERTRGSKVANPGERDAIAQVLETSALAGVLFFTNRGR
                     AYRANVHDLPKERLTAAQNLFQFGDGEKLIAVVDARLHEDHPNLVFVTAQGGVKRTSL
                     AEFAEASGRKDGIVAMKLADGDRIVSVFPGWDDYELLLVTAQGNGIRFAEEEVRPVGR
                     SAGAIRGIKLKAGDEVVGGCAVAHEEIVVIATAEGYAKRTDIDDFPEQARGGAGVKAA
                     KVDKARGPIAAVAPVADEVAFMTADGAVVVAGSSIRAAARDGGGSKVNGVSGTVLRVV
                     ALAPRDEE"
     gene            complement(2907..>3465)
                     /locus_tag="VFX21_01925"
     CDS             complement(2907..>3465)
                     /locus_tag="VFX21_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX5094737.1"
                     /translation="KILNVERASMDKVVANNEIQALIAAIGAGIGSDFDITKVRYSKI
                     VVLADADVDGGHIRTLLITFFYRQMTPLVEAGLLYVAQPPLYSVEIGGQKQYVADDAA
                     RLRIIEEHPNRQLAFARFKGLGEMDPHELRETCMDPATRNLVKLDVDQAAIADEILSI
                     LMGDDVEARRTWIQTNAKDARFLDV"
BASE COUNT          498 a         1184 c         1157 g          626 t
ORIGIN      
        1 cgacgttcga cgaagcgcgc atcgagcacc aggacgcggt cacgcgctac ggcgcgcacg
       61 gcgtaccgac gatcgtgttc gagtcgggct acgccgtata cggaccggtc gtcgtgcccg
      121 cgcccacggg cgaggacgcg ctcgcgctgt gggagctcgt cgcaggctgg cagcgcttcc
      181 cgcacctcta cgagttgcgc cacccgaaga cgcaccacga cctcgtacac gtcgcggagc
      241 agttcaacac ctacctcacg acccgcgact ggaacacagt cgaaaacccg gcgccgtaac
      301 tctttgtcgc tgtcgacctg ctcgcgtgag gcggcccgga ttgttggccg gcctcgagac
      361 tccgcgagtg cctccgcaag ctccggcacc gcctcgtgcg acttctcgcg tctgtcccac
      421 ccctattctt cgtcgcgcgg cgccaacgcg acgacgcgta gcaccgtgcc gctcacgccg
      481 ttgaccttcg agccgccgcc gtcgcgggcc gcagcccgga tggagctgcc ggccacgacc
      541 accgcgccgt cggcggtcat gaacgcgact tcgtcggcca ccggcgctac ggccgcgatc
      601 ggcccgcgcg ccttgtcgac cttggccgcc ttcacacccg cgccgccgcg cgcctgctcg
      661 gggaagtcgt cgatgtcggt gcgcttcgcg tagccctcgg cggtcgcgat cacgacgatc
      721 tcctcgtgcg cgaccgcgca gccgccgacc acttcgtcgc ccgccttgag cttgatgccc
      781 cggatcgcac ccgcactgcg cccgacgggc cgcacctctt cctcggcgaa acggatgccg
      841 ttgccctgcg ccgtcaccag caagagctcg tagtcgtccc aaccggggaa caccgacacg
      901 atgcggtcgc cgtccgcgag cttcatcgcg acgatgccgt ccttgcggcc cgacgcctct
      961 gcgaactcgg ccaggctcgt acgtttcacg ccgccctgcg cggtgacgaa cacgaggttc
     1021 ggatgatctt cgtgcaggcg cgcgtcgacg accgcaatca gcttctcgcc gtcgccgaac
     1081 tggaacaggt tctgcgctgc ggtcagccgc tccttcggga ggtcgtgcac gtttgcgcga
     1141 tacgcccgac cgcggttggt gaagaacaac acgcccgcga gcgcggacgt ttcgagcacc
     1201 tgcgcgatcg cgtcgcgctc ccccgggttc gcgaccttgc tgccgcgcgt ccgctcgggc
     1261 acggcacgga tgtagccacg tgcggtcacc gtcacgacgt agggctcgtc ctcgacgagc
     1321 gcgacgacgt cgatggtgcc ggtctcgtcg gcttcgatgc gcgtgcggcg gggcgccttg
     1381 tgcgcgtcgc ggatgacggt gagctcgtcc ttgatgacgc ccatcaggac gtccttcttg
     1441 gccaggatcc gcttcaggcc tttgatcgtc tcctccagct cttccttctc gttcgcgagc
     1501 tcttcacgac cgagctgtgt cagccggccc aacgccatgt cgaggatgtg gttggcctgg
     1561 atctccgaga aatcgaacgg cttcgccatc agcttcgtgc gagcggccgc gcggtctttc
     1621 gacgcgcgga tcgccttgac gatctcgtcg atcatgtcga gcgcccgcac gagaccctcg
     1681 acgatgtgca gccgcgcttc ggctttctcg agccggtgct gtgtgcggcg ggtgacgacg
     1741 atgacctggt ggtcgaccca cgcgtgcagc gcgtcgatca acgacacggt gcgcggcacg
     1801 ccgtcgacca acgcgaccat gttgaccggg aatgtcgtct gcgcggtcgt gtgcttgaac
     1861 atgttgttga gcacgatctg cgggttcgcg tccgggcgca gctcgatcac caggcgcgtc
     1921 ttgccctggc ccgactcgtt gcggatgtcg cgcacgccgt cgagcttgcc gttctcgacg
     1981 agctccgcga gcttgcccgc gatcgcgtcg acgctggtct ggaacggcac ttcggtgagc
     2041 acgatcgcct ggccccgccg cgggacgtct tcgatctcgt gcgtcgcacg gacgcggatc
     2101 gtgccgcggc cggtgcggaa ggcgtccttg atgccgtcgt cgccgaggat catgccgccg
     2161 gtcgggaaat cgggaccctt gatgacgcgc atgagcgcgg gcagcgtgat gtcgggctgg
     2221 tcgatcacct tcagcgccgc gttgcacact tcggcgaggt tgtggggcgg gatgttggtg
     2281 gccatgccga ccgcgatgcc ctgcgagccg ttcacgagca ggttcgggaa gcgggacggc
     2341 aagacgagcg gttcggtcgt cgacgcgtcg tagttgggct cgaagtcgac ggtgccctcg
     2401 tcgatccccg cgagcatctc catcgccagc tgcgagagcc ggcactcggt gtagcgcgcg
     2461 gctgcgggcg ggtcggaggg tgagccgaag ttgccgtgct tgtcgatgag cggtccggtg
     2521 agcgagaacg gctggcccat gcgcacgagc gcttcgtaga tcgacgagtc gccgtgcggg
     2581 tggtagttgg ccatcacgtc gccgacgacg cgcgcgcact tcacgaacgg ccggtcgggt
     2641 cgcagattcg cgtcgtgcat cgcccacagg atgcggcgct gcaccggctt caacccgtcg
     2701 cgcacgtcgg gcagcgcgcg cgcgacgatg acgctcatcg agtactcgag gaaggcgctc
     2761 tcgacctccg cgacgagcgt gcggctcggc acgtccgcgg cttcgagcgt caactgcgtg
     2821 gaggcagcct tcttccgtgc catcacagaa ctcctggtca ctcgggcgtc ttacaggggc
     2881 gaattccgcc cctgtaagac gcccgatcac acatcgagga aacgggcgtc tttggcgttg
     2941 gtctggatcc aggtgcggcg cgcttcgacg tcgtcgccca tgaggatcga gaggatctcg
     3001 tcggcgatcg cggcctggtc gacgtcgagc ttgaccaggt tgcgggtcgc gggatccatg
     3061 caggtctcgc gcagttcgtg ggggtccatc tcgcccagac ccttgaagcg cgcgaatgcg
     3121 agctggcggt tggggtgctc ttcgatgatg cgcaagcgcg ccgcgtcgtc ggcgacgtat
     3181 tgcttctgcc cgccgatctc gaccgagtac agcggcggtt gtgcgacgta gagcaacccg
     3241 gcttccacca gcggcgtcat ctgccggtag aagaacgtga tcaacaacgt tcggatgtga
     3301 ccgccgtcga catccgcgtc ggcgagcacg acgatcttgc tgtagcggac cttggtgatg
     3361 tcgaagtcgc tgccgatgcc ggccccgatc gcggcgatca gcgcctggat ctcgttgttg
     3421 gcgacgactt tgtccatcga cgcgcgctcg acgttgagga tcttc
//
LOCUS       DASUGA010000104         4771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1287411, whole genome shotgun sequence.
ACCESSION   DASUGA010000104 DASUGA010000000
VERSION     DASUGA010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287411"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VFX21_01930"
     CDS             <1..595
                     /locus_tag="VFX21_01930"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5094738.1"
                     /translation="EDRCRRARAALGIDPEQALIVAIGRQEWQKGHDVLLAAVPELLR
                     AEPGAVVAIAGRAGGQTERLRETITRENLSDHVRLLGFRDDAPELLSAADVFAFPSRW
                     EGLPGTVLEAMALEAPIVATDLPMTREVVTREDAMLVPPDDAAALAAAIVDCLHDRAA
                     AQHRARAARRRVAHEFTIEQSALGMLRFFERALGSSG"
     gene            592..1506
                     /locus_tag="VFX21_01935"
     CDS             592..1506
                     /locus_tag="VFX21_01935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5094739.1"
                     /translation="MTGAAPMPRVSIVIPTYQRRDLVVRAVRSVLAQDHRGFELIVVD
                     DGSTDGTGDALRAMRDERIRLIESEHLGATAARNSGARAATCPWVTFLDSDDTVVRDW
                     LSSMVPELNEPNTALVSCGYVERFEGETVDRRHRMPRPASASVGPIVELIEIGGTYIV
                     LRDLFLDIGGFDVAQRASQHQELALRLGPEIVRRGMRSAAVMRPLVEHWIGRDDHIRS
                     DDDAVLSGCMRLLERHRDRLRLDPPMLANTAATAAYRAVRIGEFSTARQLMVLAVRTQ
                     PRNARHWLRLGALAAPRAAQRRALRTRS"
     gene            1517..2599
                     /locus_tag="VFX21_01940"
     CDS             1517..2599
                     /locus_tag="VFX21_01940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5094740.1"
                     /translation="MSIRIVALGSTTRRNRIGRTYSLWLTARAANIPFLYIGVDDGPI
                     WEPLRDNEAFLADVRTAADVADLERQVARELDPDTVLLVCKPRPEILRVALRLERFAP
                     VLVDVDDPELLDPWIDNSLLLRVKRVLRTGPSKFRFGWARRVVAKMDVIASSPSLQER
                     YGGIVVPHVREDDALPRARDGSERQFRVGFVGTPRNHKGVPQLRAAVALLAQQRDVRL
                     CITAPRPEDAQPWEEWVGYTTMEGGRRVLAESDAVAIVSQPGIWGELQFPVKLVDAML
                     AEVPVVITPRGPLLWAMGGTGVVVSDGSVDEIVSALTRLADDPAFARELAVAARQRAL
                     GMFTPAAVAPHFREAVERVYIKRTRT"
     gene            complement(2615..3481)
                     /locus_tag="VFX21_01945"
     CDS             complement(2615..3481)
                     /locus_tag="VFX21_01945"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5094741.1"
                     /translation="MPVQETNNNGRLPDFIVIGAMKAATTSLWKYLRAHPQVFMPDLK
                     EMQFFSNPGRLGRDLQWYREQFGPAGDAVSVGEASTNYTKYPRLVGTPERMAAAVPDV
                     RLVYLVRDPIERIRSHYLHVAYGHAEHRPLVEAVREDPEYVSISRYRMQIDQYLEHFD
                     ASQLLVVTSEELKANRKRTMEQVFRFVGVEPALTAPERDREVHRTADKRIDTPVMTRL
                     RRLPMSESLRRFTPAPLRRFASRTVTRPVTAQVDATITDELRRQLVEELADDVAGLRP
                     FLGADFDGWGIA"
     gene            3875..>4771
                     /locus_tag="VFX21_01950"
     CDS             3875..>4771
                     /locus_tag="VFX21_01950"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX5094742.1"
                     /translation="MTAQLQRFIDAVPNGTIISFPGAARYRLGGTLRLVSRRAITLDG
                     NGATLFATPSPLRERAQLELDESFDIVVQGFRIVGSNPNAGTGEDAYNPDYEAQHGIN
                     VLGGQRINIVRNEIRDVYGDFVYIGHGRPRRDDEPSAVTIRDNLMHANGRQGITVTHG
                     FDIDIVDNSIYDVRRTTIDFEPNVPEDTVAGVQIRSNRIGAGRLLFVGAEGNGVVNDI
                     AVVGNKLQGRTMSASFRAPYGTRRSGIVWVGNVSDKGSGNPDGATIGIHGYDHIHVSD
                     NVQPSNIRRDMHMVAAYQLCDYE"
BASE COUNT          769 a         1548 c         1617 g          837 t
ORIGIN      
        1 cgaagatcgg tgccgccggg cccgagcggc actcggcatc gatccggagc aagcgttgat
       61 cgtcgcaatc gggcggcagg aatggcagaa gggtcacgac gtcttgttgg ccgcggtgcc
      121 cgagctgttg cgggcggaac ctggtgccgt cgtcgccatc gcggggcgcg cgggaggcca
      181 aaccgaacgg ttgcgtgaga cgatcacgcg cgagaacttg tccgatcacg ttcgcttgct
      241 cggctttcgc gacgacgcgc cggagctgct gagtgcagca gacgtcttcg cgtttccgtc
      301 gcggtgggag ggtctcccgg gcacggtgct cgaggcgatg gcattggagg ctccgatcgt
      361 cgcaaccgac ctccccatga cgcgagaggt cgtcacccgc gaggacgcga tgctcgtgcc
      421 gcccgacgac gcggctgcgc tcgcggccgc gatcgttgat tgcctgcacg accgtgccgc
      481 cgcacagcat cgcgcgcgag cggcgcgccg gcgcgtcgcg cacgagttca cgatcgagca
      541 gtcggcgctc gggatgcttc gcttcttcga gcgcgctcta ggatcgagcg ggtgaccggc
      601 gccgcaccga tgccgcgggt gagcatcgtg atcccgacct atcaacggcg tgatctcgtc
      661 gtgcgggcgg tgcgttcggt gctggcgcag gatcaccgag gtttcgagct catcgtcgtc
      721 gacgacgggt cgacggacgg gaccggagat gcactccggg ccatgcgcga cgaaaggatc
      781 cggctcatcg agtccgagca tcttggtgcg acggcggcgc ggaactcggg tgcgcgcgcc
      841 gcgacttgcc cgtgggtcac cttcctcgac agcgacgaca ctgtcgtgcg cgattggctc
      901 tccagcatgg tgcccgagct gaacgaaccg aacacggccc tcgtcagctg tggctacgtc
      961 gagcgcttcg agggtgagac ggtcgatcgg cgacaccgca tgccgcgccc ggcgagcgcg
     1021 tcggtcgggc cgatcgtcga gctcatcgag atcggcggca catacatcgt gctccgcgac
     1081 ctcttcctcg acatcggcgg cttcgacgtt gcgcaacgcg ccagccagca tcaggagctg
     1141 gcattgcgcc tcggtccgga gatcgtccgg cgcgggatgc ggtccgctgc tgtgatgcga
     1201 ccgttggtcg aacactggat cggtcgcgac gatcacatcc gtagcgacga cgatgcggtg
     1261 ttgtccggat gcatgcggct gctcgaacgg caccgcgacc gtctgcgact cgatccaccc
     1321 atgctcgcca acacggccgc caccgccgca tatcgcgcgg tgcggatcgg cgagttctcg
     1381 actgcccgac agctcatggt gttggcggtc cggacgcaac cgcgcaatgc acggcactgg
     1441 ctgcgcctcg gagcgctcgc ggcaccgcgc gccgcgcaac gccgggccct gcggacacgc
     1501 tcgtgacggg tattcgatga gcatccgcat cgtcgcgctg ggcagcacga cgcgccgcaa
     1561 tcgcatcggt cgcacatact cgctctggct cacggcacgc gccgccaaca ttccctttct
     1621 ctatatcggt gtcgatgacg gacccatctg ggagcccctg cgcgacaacg aggcgtttct
     1681 tgccgacgtc cgcactgccg cggatgtcgc cgacctcgaa cgacaggtcg cgcgcgagct
     1741 cgaccctgac accgttctcc tcgtgtgcaa gccccgtccc gagatcttgc gcgtcgcctt
     1801 gaggctcgaa cggtttgcgc cggtgctcgt cgacgtcgac gatccggagt tgctcgaccc
     1861 gtggatcgac aacagcctgc tccttcgcgt caagcgtgtc ctgcgtacgg gtccgtcgaa
     1921 gttccgcttc ggctgggccc ggcgagtcgt cgcgaagatg gacgtcatcg cctcgagccc
     1981 gtcgctccag gagcgatacg ggggcatcgt cgtgccgcat gttcgcgagg acgatgcgtt
     2041 gcctcgcgcg cgcgacggtt ccgaacgaca gttccgggtg ggattcgtcg gaacgccgcg
     2101 caaccacaaa ggcgtccccc aattgcgagc cgcggtcgcg ctgctcgcgc agcagcgcga
     2161 cgtgcgcctg tgcatcaccg cgccacgacc tgaggacgca cagccgtggg aggagtgggt
     2221 cggctacacg acgatggaag gcggccgccg cgtcctcgcc gaaagcgacg ccgtcgcgat
     2281 cgtttcgcag cccggcatct ggggcgaatt gcagtttccc gtgaagctcg tcgacgcgat
     2341 gcttgccgag gtgccggtcg tcatcacgcc tcggggaccg ctcttgtggg cgatgggcgg
     2401 caccggcgtg gttgtctccg acggttcggt cgacgagatc gtgtccgcac tcactcgtct
     2461 cgcggacgac cccgcgttcg ctcgtgagct cgcagttgcc gcacggcagc gtgcgctcgg
     2521 catgttcacg ccggccgccg tggctccgca ctttcgcgaa gccgtcgaac gcgtgtacat
     2581 caagcgcacg cgcacctgat ctaccggggc agcgtcaggc gatgccccaa ccatcgaagt
     2641 cggcaccgag aaacggtcgc agcccggcga cgtcgtcggc cagttcctcg acgagttgac
     2701 ggcgtagctc gtcggtgatc gtcgcgtcga cttgcgccgt cacgggacgg gtgacggtgc
     2761 gactcgcgaa tcgccgcagc ggcgccggcg tgaaccggcg cagcgactcg gacatcggca
     2821 gccggcgcag tcgcgtcatc accggcgtgt cgatgcgctt gtcggccgtc cggtggactt
     2881 cgcggtctcg ttcgggcgca gtgagcgccg gttcgacacc cacgaaccgg aagacctgct
     2941 ccatggtgcg cttgcggttc gccttcaatt cctccgatgt cacgacgagc agttgagacg
     3001 cgtcgaagtg ctcgaggtac tggtcgatct gcatccggta ccggctgatc gagacgtact
     3061 cggggtcttc ccgaaccgcc tcgacgagcg gccggtgttc ggcgtgcccg taggcaacgt
     3121 gcaggtagtg cgagcggatt cgttcgatcg ggtctctgac gagatacacg agccgaacgt
     3181 cgggaacagc cgcggccatg cgctcgggcg tgccgacgag acgcgggtac ttggtgtagt
     3241 tcgtcgacgc ttcgccgacg gataccgcgt cgccggccgg tccgaactgt tcgcggtacc
     3301 actgcagatc gcgaccaaga cgacccggat tcgagaagaa ctgcatctcc ttgaggtccg
     3361 gcatgaacac ctgcgggtgt gcgcgcaggt acttccacag gctcgttgtg gccgccttca
     3421 tcgcgccgat gacgatgaaa tcggggagcc gtccgttgtt gttcgtctcc tgcacgggca
     3481 tgagcggcga ctttcacgat tcggcgcagg tcagtacggc gtcgcatgcc gatcggcgcc
     3541 ctgtaagacg aggagtccgg tgcagcgtag acgacaggtc cgctgcggtt tcgccgtgtg
     3601 cgcgggtttc gtgccgggct gtcgaggcct tcaaggtcga tggtagcggt ggtcgatacc
     3661 tacctcgacg caccgtgcgg agaggtgcga atgggggaaa cacatgacgc aacgacgtgt
     3721 tcgtcgcgcc ttcatcggtc ttggtgtgct cgttctcgcg ttcgcaagcg cagcgtgcga
     3781 accgcagttg gccttcccgc ggtaccggtc ggacggcact gaggtcccga cggttcgcgt
     3841 tccgggttcg atcgatgcgt cggggcgcac cgacgtgacg gcgcagttgc agcggttcat
     3901 cgatgcagtt cccaatggaa cgatcatctc gttcccgggt gctgccaggt atcgactcgg
     3961 cggcacgttg cgcctcgttt cccggcgggc gatcacgctc gacggtaacg gtgcaaccct
     4021 cttcgcgacg ccttcaccct tgcgtgagcg tgcgcaactc gagctcgacg aaagctttga
     4081 catcgtcgtg caggggttca ggatcgtcgg ttcgaacccg aatgcgggta cgggtgagga
     4141 cgcgtacaac ccggactacg aggcgcagca cgggatcaac gtgttgggcg ggcagcgcat
     4201 caacatcgtg cgcaacgaga tcagagacgt ctacggcgac ttcgtctaca tcggtcacgg
     4261 gcgcccgcgg cgcgatgacg agccgtcggc ggtcacgatt cgcgacaacc tgatgcacgc
     4321 gaacgggcgc cagggcatca cggtgacgca cgggttcgac atcgacatcg tcgacaacag
     4381 catctacgac gtacgtcgta cgacgatcga cttcgagccg aacgtgcccg aggacacggt
     4441 tgccggagtg cagatccgca gcaacaggat cggcgcgggg aggttgctgt tcgtcggtgc
     4501 cgaaggcaat ggcgtggtca acgacatcgc cgttgtgggc aacaaacttc agggccgtac
     4561 gatgtcggcc agcttccggg cgccctacgg cactcgtcga tcgggcatcg tctgggtcgg
     4621 caacgtgagc gacaagggct ccggcaaccc tgacggcgcg accatcggca ttcatggcta
     4681 cgaccacatt catgtgagcg acaacgtgca gccttcgaac atccggcggg acatgcacat
     4741 ggtcgccgca taccaactct gcgattacga g
//
LOCUS       DASUGA010000105         2154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_105612, whole genome shotgun sequence.
ACCESSION   DASUGA010000105 DASUGA010000000
VERSION     DASUGA010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105612"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VFX21_01955"
     CDS             <1..302
                     /locus_tag="VFX21_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5094743.1"
                     /translation="GQVNYAAAKAGLVGLTRSVARELASRGVTCNVVAPGPIVTAMTA
                     EMPDDWRETIHAAVPLGRFGTPEECAAVVTFLCSEAAGYVTGAVVPVDGGLSMGH"
     gene            331..576
                     /gene="acpP"
                     /locus_tag="VFX21_01960"
     CDS             331..576
                     /gene="acpP"
                     /locus_tag="VFX21_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX5094744.1"
                     /translation="MDRADALSTIREVAAEVLSVDPDQVVETARFKEDLDADSLDLVE
                     LVMGLEERFDIEVPEEDLEGVTTIGQAVDLVLSKAGA"
     gene            581..1837
                     /gene="fabF"
                     /locus_tag="VFX21_01965"
     CDS             581..1837
                     /gene="fabF"
                     /locus_tag="VFX21_01965"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX5094745.1"
                     /translation="MANEHLDHRGRHRVAITGIGLKTPAGQDVETFWSTLNANRSVAA
                     ELQRFDPSELPVRFGCEVLDYDVTQYIGPKEARRLDRVTHLGLGAAADALADAGELRN
                     NPARSAVIMGTGIGGLNTLEEQTIVYKDKGPNRVSPFFVPMMMANATAGNIAMQFGWT
                     GPNLCIATACAASAHAIGEAARLVREGTADVVMTGGSEACLTPVAISAFGRMTALSNR
                     NDAPELASRPFDADRDGFVMGEGAAALVLEPWERAVARGARIHGEVIGYGRNADAFHI
                     TAPSPGGEGASACMQLALDDAGLSPTAIGHVNAHGTSTPLNDAAEAEAIRKVFGGDNA
                     VPVTSTKGVTGHLIGAAGATEAIAAVLAMARGSVPPTANLERIGDDIMLDVVHDAPRE
                     LAAAPSLSNSFGFGGHNATLVFAPGA"
     gene            1834..>2154
                     /locus_tag="VFX21_01970"
     CDS             1834..>2154
                     /locus_tag="VFX21_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX5094746.1"
                     /translation="MNDTPLATEADAAVSAAVREIDGRRVVWFRIAGGKHHGAIGVNG
                     SATVESAVRLAGRLQVPLVGELDTSGAELHDGVAALHAWGRLARALVELSGSVPTLVA
                     VTGAC"
BASE COUNT          364 a          736 c          736 g          318 t
ORIGIN      
        1 ccgggcaggt gaattacgcg gccgccaaag cagggctcgt cgggctgacg agatcggtcg
       61 cccgcgaact cgcgagccgg ggcgtcacct gcaacgtcgt cgcacccggg cctatcgtga
      121 cggcgatgac ggccgaaatg cccgatgact ggcgggaaac gattcacgcc gccgtaccac
      181 ttggccggtt cggcacaccc gaagagtgtg ccgcggtcgt tacgttcttg tgctcagagg
      241 ccgcggggta tgtcacgggt gcggtcgtgc ccgtcgacgg cggcttgagc atgggccact
      301 gatccgaagc aaccgaggaa ggactcaccc atggaccgcg ccgacgcgct cagcacgatt
      361 cgcgaagtcg ccgccgaagt gctcagcgtc gaccccgacc aggtcgtcga aaccgctcgc
      421 ttcaaagaag acctcgacgc cgacagtctc gacctcgtcg agctcgtcat gggtctcgaa
      481 gagcggttcg acatcgaagt gcccgaggaa gacctcgaag gcgtaacgac gatcggccaa
      541 gcggtcgacc tcgtgttgtc aaaggcaggc gcataagaac atggcgaatg agcacctcga
      601 ccaccgcggg cggcaccgcg tcgcgatcac gggcatcggg ctgaagacgc ccgcgggcca
      661 ggacgtcgag acgttctggt cgacgttgaa cgcgaaccgt tctgtcgcgg cggagctgca
      721 acgcttcgac ccgtccgagc tgccggttcg gttcggttgt gaggtgctcg actacgacgt
      781 cacgcaatac atcggaccga aggaagcgcg ccggctcgac cgcgtgacgc acctcggcct
      841 cggcgcggca gccgatgcgc tggccgacgc gggtgaactc cggaacaatc cggcgcgcag
      901 tgccgtgatc atgggaaccg gcatcggtgg cctgaacacg ctcgaagaac aaacaattgt
      961 ctacaaggac aagggcccaa atcgcgtcag cccgttcttc gtgccgatga tgatggccaa
     1021 cgcgaccgcc ggcaacattg caatgcagtt cggctggacc ggcccgaatc tctgcatcgc
     1081 caccgcgtgc gcggccagtg cgcacgccat cggcgaagcg gcccggctcg tgcgcgaagg
     1141 caccgccgac gtggtcatga ccggcggatc cgaagcgtgc ctcacgcccg tggccatctc
     1201 cgcgttcggg cgcatgacgg cactcagcaa ccgcaacgac gctcccgaac tcgcgtcccg
     1261 tcccttcgac gctgatcgcg acgggttcgt catgggcgag ggcgcggccg cgctggtgct
     1321 cgagccctgg gaacgggccg tcgcccgcgg cgctcgcatc cacggcgagg tcatcggcta
     1381 cgggcgcaac gctgacgcgt tccacatcac cgcgccgtcg ccgggtggcg aaggcgcatc
     1441 ggcgtgcatg caactcgcac tcgacgacgc cggcctctcc cccacggcca ttggtcacgt
     1501 gaacgcgcac ggcacgtcga cgccgttgaa cgacgccgcg gaagccgaag ccatccgcaa
     1561 ggtgttcggt ggcgacaacg cggtgcccgt gacgtcgacg aagggcgtca ccggccacct
     1621 catcggtgcc gccggcgcga ccgaagcgat cgcagcggtg ctggcgatgg cgcgcggttc
     1681 ggtgccgccc accgcgaacc tggaacgtat cggcgacgac atcatgctcg acgtcgtgca
     1741 cgacgccccg cgtgagctgg ccgcggcgcc gtcgttgtcg aactcgttcg ggttcggcgg
     1801 tcacaacgcc acattggtgt tcgcacccgg cgcgtgaacg acaccccgct cgcgaccgag
     1861 gccgatgccg cggtttcggc tgccgttcgc gagatcgacg gccgacgagt ggtgtggttc
     1921 cgcatcgccg gcggcaaaca ccatggcgcg attggcgtca acggcagcgc caccgtcgag
     1981 tcggcggtac ggctcgcggg ccggttgcag gttccgctcg tgggtgaact cgacacgtcg
     2041 ggcgccgagc tgcacgacgg cgttgccgcg ctgcacgcgt ggggacggct cgcccgcgcg
     2101 ctcgtcgagc tgtccgggtc ggtgccgacg ctggtcgccg tcaccggcgc gtgc
//
LOCUS       DASUGA010000106         2227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_123993, whole genome shotgun sequence.
ACCESSION   DASUGA010000106 DASUGA010000000
VERSION     DASUGA010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123993"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VFX21_01975"
     CDS             <1..282
                     /locus_tag="VFX21_01975"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEX5094747.1"
                     /translation="RVVANRVERALAAVPPPEAPFDLVFCDPPYDLRDADVDAALQLL
                     ARPGWITDDTFVSVERPAGARIEAPPPLRTHWERTFGDTLVVFLATDED"
     gene            296..793
                     /gene="coaD"
                     /locus_tag="VFX21_01980"
     CDS             296..793
                     /gene="coaD"
                     /locus_tag="VFX21_01980"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013807954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX5094748.1"
                     /translation="MATALCPGSFDPVTNGHLDIIERTARHFDQVIVAVIRNPQKNSL
                     FTLEERQEMLHEVTAHLPNIRIEFFKGLLVDFAKDHGANAIVKGLRAISDFDYELQMA
                     QMNQRLSGIDTFFISTSPQHSFLSSSLVKEVARFGGDVSSMVPKTVVDRLAKVFAERP
                     DAKER"
     gene            793..1332
                     /locus_tag="VFX21_01985"
     CDS             793..1332
                     /locus_tag="VFX21_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094749.1"
                     /translation="MVLEDAPDTAPDTRQLLLRLQELLENARAMPLSASVMVNREEFG
                     ELLLEAIEGLPEELREARWLLKERDDVLERARREADRIIEAARVRAERMIERQEIVRE
                     ARRERDQIIETAERNAAQIRHEAEDYVDRKLAAFEVVLDRTLTQVHKGRERLQVHIEP
                     AEAAEEDSDEVAAFFDQDE"
     gene            1495..2001
                     /locus_tag="VFX21_01990"
     CDS             1495..2001
                     /locus_tag="VFX21_01990"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX5094750.1"
                     /translation="MSSANPLRISVADLLRRPGATRSVDIDAPVTGLDNGVAVVGPGE
                     PVHVGVTLERISEGIVVRGDVDVTWRAECSRCVKPIAGTISVHVDELYEPNPLAGETY
                     GLDDDILDLEPMVRDAVALELPAAPLCRDDCAGLCPRCGADHNETACDCVVDETDSRW
                     AALRSLEI"
     gene            2014..2193
                     /gene="rpmF"
                     /locus_tag="VFX21_01995"
     CDS             2014..2193
                     /gene="rpmF"
                     /locus_tag="VFX21_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013251914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX5094751.1"
                     /translation="MPVPKRKTSRSATRSRKSANMRLARPAHSLCPQCGNSKLPHTVC
                     GTCGWYRGRRVLDVD"
BASE COUNT          378 a          766 c          750 g          333 t
ORIGIN      
        1 cgcgtcgttg cgaaccgggt cgagcgagcg ctcgccgccg tgccgccacc ggaggcgccc
       61 ttcgaccttg tcttctgcga cccgccgtac gacctgcgcg acgcggacgt cgatgccgcg
      121 ctgcagttgc tggcgcggcc cggatggatc accgacgaca cgttcgtgag cgtcgagcgg
      181 cctgcaggcg cccggatcga ggcgccgcca ccactccgaa cccactggga acgtacattc
      241 ggagatacgc tcgtcgtctt cctggcgacc gacgaagact gagagaggcg cccccgtggc
      301 caccgccttg tgtccgggtt cgttcgaccc cgtgacgaac ggtcatctcg acatcatcga
      361 acgcacggcg cgtcacttcg accaggtgat cgtcgcggtc atccgcaatc cgcagaagaa
      421 ctcgctgttc accctcgaag agcgccagga gatgctgcac gaggtcaccg cgcacctccc
      481 gaacatccgg atcgagttct tcaagggcct gctcgtcgac ttcgccaagg accacggcgc
      541 caacgcgatc gtgaagggcc tgcgggcgat ctccgatttc gactacgagc tccagatggc
      601 gcagatgaac cagcgcctgt ccggaatcga cacgttcttc atctcgacga gcccgcaaca
      661 ctcgttcttg tcgtcgagtc tcgtgaaaga agtcgcacga ttcggcggcg acgtctcgag
      721 catggttccc aagaccgtcg tggatcgtct cgcgaaggtg ttcgccgagc gtcccgacgc
      781 gaaggagcgg tgatggtgtt ggaagacgca cccgatactg cacccgatac ccgacaacta
      841 ttgctgcggt tgcaggaact gctcgagaac gcgcgcgcca tgccgctcag tgcatcggtc
      901 atggtgaacc gcgaggagtt cggcgagttg ttgctcgaag cgatcgaggg cctgcccgaa
      961 gaactgcgcg aggcgcgctg gttgttgaaa gagcgcgacg acgtgctcga gcgggcgcgc
     1021 cgcgaagccg accgcatcat cgaggcggcg cgtgtgcgcg ccgagcggat gatcgagcgc
     1081 caggagatcg tgcgggaggc gcgccgcgag cgcgaccaga tcatcgagac cgcggagcgc
     1141 aacgcggccc agatccgcca cgaagccgag gactacgtcg accgcaagct ggcggccttc
     1201 gaggtggtgc tcgaccggac gctgacccag gtccacaagg gccgcgagcg cctccaggtg
     1261 cacatcgaac cggcggaggc cgccgaggaa gacagtgacg aggtcgcggc cttcttcgac
     1321 caggacgaat agcccgaacg gcccggtcct cgggccccgc aaggagccgg cctgcacggc
     1381 gtcacctgct acgatccgcg gccagcctcg tcgccggcgg tactgtccgc gcgctcgacg
     1441 ggtgtccgtt ccccggccgt gagaatccgg ccgatcctgc cttgcgagac cggcatgtcc
     1501 agcgccaatc cacttcgcat ttcggtcgct gacctgctca gacggcccgg cgcgacccga
     1561 tcggtcgaca tcgacgcccc ggtcacgggc ctcgacaacg gcgtcgcggt ggtgggcccc
     1621 ggcgagcccg tccacgtggg cgtgaccctg gagcgcatct cggagggcat cgtcgtccgg
     1681 ggcgacgtcg acgtcacgtg gcgggccgag tgcagccggt gtgtgaagcc gatcgcgggc
     1741 acgatctcgg tccacgtcga cgagctctac gagccgaacc cgctcgcggg cgagacgtac
     1801 ggcctcgacg acgacatcct cgacctcgaa ccgatggtgc gcgacgcggt ggcgctcgag
     1861 ctgcccgccg cgccgctgtg tcgcgacgac tgcgccgggc tgtgcccgcg ctgcggcgcc
     1921 gaccacaacg agacggcgtg cgactgcgtc gtcgacgaga ccgattcccg atgggcggcc
     1981 ctgcggtcgc tcgagatctg acaggagcaa tcgatgcccg tcccgaagcg caagacgagc
     2041 cgttcggcga cgcgttcgcg caagtccgcg aacatgcgtc tcgctcgccc cgcgcactcg
     2101 ctctgcccgc agtgcgggaa ctcgaagttg ccgcacaccg tgtgcggcac gtgcggctgg
     2161 taccgaggcc gacgggtcct cgacgtcgac tgagacgacg gcgcagtcga tggcggcact
     2221 cgggaac
//
LOCUS       DASUGA010000107         2262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_264956, whole genome shotgun sequence.
ACCESSION   DASUGA010000107 DASUGA010000000
VERSION     DASUGA010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264956"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VFX21_02000"
     CDS             complement(<1..400)
                     /locus_tag="VFX21_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094752.1"
                     /translation="MTSRTARRAETRIVVAFLCTAASGVALAVVYWNGGNPQLEGLFL
                     GLGLGGLFVGFVTWGNHLLPQGPETGPREEMPSEPGAVEALEVDVERGGALTRRKLIL
                     GSLGAAAVAMITAALFPIRSLGPRPGRALLE"
     gene            complement(397..1191)
                     /locus_tag="VFX21_02005"
     CDS             complement(397..1191)
                     /locus_tag="VFX21_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5094753.1"
                     /translation="MGFRVARLAAVAGVVSCCALFTLSTDRIRAGAQVDDRSAPAIYR
                     SDCSYCHGVDAGGTRYGPSLAGSGRAGVDYALSTGRMPLIGAGRTAEAGRPIRPLPGQ
                     LAAQADAQPARHEPAYDDATIRAIVDYVATLTGQTDPDIPTLRPGSVSSGGELYRLQC
                     AACHAWAGDGGALLHREAPSVHPATPTQAAEAMRVGPGQMPSFGQAALTDRQLDDVVA
                     YVEYLEDPEDRGGHPLGHLGPFAEGGVALVIVAGLMLMTRWIGERG"
     gene            1282..>2262
                     /locus_tag="VFX21_02010"
     CDS             1282..>2262
                     /locus_tag="VFX21_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5094754.1"
                     /translation="MVKPAILTVDDDPAVSQAIARDLRRQYGAEFQVVRAGSGSEALE
                     VLSTLQLRDRPVALIASDQRMPRMTGVEFLEQARSHAPDAKLVLLTAYADTDAAIKAI
                     NDIDLDHYLMKPWDPPEDRLYPVLDDLLSDWVRTHPSDQAGVRVIGHRWSDRSHEIKT
                     FLARNYVPYQWLDLERDEEARRLHEAANATATDLPLVLVPEADPLRAPSTLDLADALG
                     LRTTAQQPLYDVCVVGGGPAGLAAAVYAASEGLQTVVVEGQAPGGQAGQSAAIENYLG
                     FPKGLSGADLTHRAVAQAQRFGAEMVLARRCIGFETRGHVRAVSLDGQNEI"
BASE COUNT          355 a          809 c          795 g          303 t
ORIGIN      
        1 tctcgaggag cgcgcgaccc ggtcgcggcc cgagcgaccg gatcgggaag agcgcggcgg
       61 tgatcatcgc gaccgccgcg gcaccgaggc tgccgaggat cagcttgcgg cgcgtgagcg
      121 cgccgccgcg ttccacgtcg acctcgagcg cttcgaccgc gcccggttcg ctgggcatct
      181 cctcgcgcgg tccggtctcc ggaccctgcg ggagcaagtg gttgccccac gtcacgaagc
      241 cgacgaacag cccgccgaga ccgaggccga gaaagaggcc ttcgagctgc ggatttccgc
      301 cgttccagta cacgaccgcg agggcgacgc cgctcgccgc ggtgcacaag aacgcgacga
      361 cgatgcgagt ctccgcgcgg cgtgcggtgc ggctcgtcac ccgcgctcgc cgatccagcg
      421 cgtcatgagc atcaagcccg cgacgatcac aagtgcgaca ccaccctcgg cgaacgggcc
      481 gagatgtccg agcggatgac cgccgcggtc ctcgggatcc tcgaggtact cgacgtacgc
      541 aacgacgtcg tcgagttgtc ggtcggtcag cgcggcctgc ccgaaagacg gcatctggcc
      601 gggcccgacc cgcatcgcct ccgcggcctg cgtcggcgtc gcgggatgca ccgacggtgc
      661 ttcccgatgc aacagcgcgc caccgtcgcc cgcccacgca tggcacgccg cgcactgcaa
      721 tcggtagagc tcgccgcccg aggacaccga accgggtcgc agcgtcggga tgtccgggtc
      781 ggtctgaccg gtcaacgtcg cgacgtagtc gacgatcgcg cgaatcgtcg cgtcgtcgta
      841 cgcaggttcg tgacgcgcgg gctgcgcgtc ggcttgcgcc gccagttgcc ccgggagcgg
      901 ccggatcgga cggcccgctt cggccgtgcg gccggcgccg atgagcggca tccggccggt
      961 cgacaatgcg tagtcgacac cggcgcggcc ggaaccggcg aggctcgggc cgtagcgcgt
     1021 gccgccggcg tcgacgccgt gacagtacga gcagtcggac cggtagatgg ccggtgcgga
     1081 ccgatcgtcg acctgggcac cggcgcggat gcgatccgtc gacagcgtga agagcgcgca
     1141 acaggagaca acgcccgcga ctgcagccag ccgcgcgacg cggaagccca tgtggaagcg
     1201 ctaccgccgg ccgcccgttc gcgaccgaat ctgaatcact gctgagaagc acgccgccgc
     1261 tcgggcgcta acctccggcg ggtggtcaag cccgcgatcc tgacggtcga cgacgacccg
     1321 gcggtgtcgc aggccatcgc gcgcgacttg cggcgccagt acggcgccga gttccaggtc
     1381 gtgcgggccg gctccgggag cgaagcgctc gaggtgctga gcacgttgca gctgcgcgac
     1441 cggccggttg cgctgatcgc ctccgatcag cgcatgccgc ggatgaccgg tgtcgaattc
     1501 ctcgaacagg cccgctccca cgcgcccgac gccaagctcg tgttgctcac cgcgtacgcg
     1561 gacactgacg ccgcgatcaa ggcgatcaac gacatcgatc tcgaccacta cctgatgaag
     1621 ccgtgggatc cgccggagga ccgcctgtac ccggtgctcg acgacttgtt gagtgactgg
     1681 gtccggacgc atccctcgga ccaggcgggc gtgcgagtga tcggccaccg ctggtccgac
     1741 cgcagtcacg agatcaagac gttcctggcc cgcaactacg tgccctacca gtggctcgat
     1801 ctcgaacgcg acgaagaggc gcggcgcctg cacgaggccg cgaacgccac cgcaaccgac
     1861 ctgccgctcg tgctcgtccc cgaagccgat ccgttgcgcg cgccgtcgac gctcgatctg
     1921 gccgacgcgc tcggtctgcg cacgaccgcg caacaaccgc tgtacgacgt ctgcgtcgtc
     1981 ggcggtggcc ccgcgggtct cgcggccgcg gtgtacgccg cgtcggaggg attgcagacc
     2041 gtcgtcgtcg aaggacaagc gccgggcggg caagccggcc agagcgcggc gatcgagaac
     2101 tatctcggct tccccaaggg tctgtcgggg gccgacctca cgcaccgagc ggtcgcgcag
     2161 gcgcagcgct tcggcgcgga gatggtgctc gcccgccggt gcatcggctt cgagacccgc
     2221 gggcacgtgc gcgcggtgag tctcgacggt cagaacgaga tc
//
LOCUS       DASUGA010000108         7314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_52791, whole genome shotgun sequence.
ACCESSION   DASUGA010000108 DASUGA010000000
VERSION     DASUGA010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7314
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52791"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFX21_02015"
     CDS             <1..342
                     /locus_tag="VFX21_02015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007144087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX5094755.1"
                     /translation="IGGLGGNSIWLTTDDGNAYYYAHLDGFAGEFDANNQRRVVKGEI
                     VGYVGNTGNAAGGPTHTHFEIRPQNIGPVNPYPMLKEMCAVQGGFVPPPEQSVDVMGS
                     TTTTTTLPVPG"
     gene            complement(577..1224)
                     /locus_tag="VFX21_02020"
     CDS             complement(577..1224)
                     /locus_tag="VFX21_02020"
                     /inference="COORDINATES: protein motif:HMM:NF019151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HEX5094756.1"
                     /translation="MRGRRLVWVGVVVAIVVAVLLVRALDSGKSPAEEPTVPGTLDAL
                     HVAEPDLSTPYDRDRFGGGWIDADGDCLDTRQEVLAVESTTPVVMTGCAVQSGSWTDP
                     WSGRSYTDPGEVEIDHTVALADAWRSGASTWTDARREQFANDLTDPMILNALASAENA
                     AKSDKGPDAWKPPDQSSWCVYASAWVRIKLRWDLSATRAEWNALRTMLETCAGAP"
     gene            1437..1664
                     /locus_tag="VFX21_02025"
     CDS             1437..1664
                     /locus_tag="VFX21_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094757.1"
                     /translation="MNCDAPLPVDAIDVGADFAVKTVIEASAGRASVRILMVNGQEVH
                     RCLVADHRDFLPAAEPSKDSAAVHARDATWA"
     gene            2034..3164
                     /locus_tag="VFX21_02030"
     CDS             2034..3164
                     /locus_tag="VFX21_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094758.1"
                     /translation="MADLVIHAGMPKTGSSAVQRWLKSNLDAIAANGFQVRRRPRGLD
                     EATCLDEVTRPGSHIGVKSYVDDLLGNAQEQRALLDDLHALPGTALLTSEALNNLFHR
                     DAVWLSALNDYAAIHDVRVAYYVRPQHDAMEASWRQWGFRRPMRPSVYLERREGRRLD
                     FLSTLMRVRECAPNVSFEMRGFRRDLLVGGDIVVDFADTFLGMHAEPVPSVNEGLPLF
                     ICVLIQARGASPLWESHHENATLRRVQEIVSRWDMPEPEDIQESLLVLQRHCRSRYED
                     DNRKLISLLNWPTEDYVPPTKEAGDDRLSRLDVLWRPRPEGHLLRQALADIIATSDTR
                     DARQSRPSSDRAGERVTLRSKLRTATRHGAKALRRSRGKPSA"
     gene            complement(3292..3374)
                     /locus_tag="VFX21_02035"
     tRNA            complement(3292..3374)
                     /locus_tag="VFX21_02035"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3339..3341),aa:Leu,seq:caa)
     gene            3417..3674
                     /locus_tag="VFX21_02040"
     CDS             3417..3674
                     /locus_tag="VFX21_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094759.1"
                     /translation="MVEFVVGLVVGASAAVLLLIVFGGSRRIRAEKPLERDVETQILL
                     GEDPDKTTTPSASPSKNHPRNYSADELAQLRRLGTSRRKNR"
     gene            complement(3675..4100)
                     /gene="fabZ"
                     /locus_tag="VFX21_02045"
     CDS             complement(3675..4100)
                     /gene="fabZ"
                     /locus_tag="VFX21_02045"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF000582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX5094760.1"
                     /translation="MSDQYAEALARLPHGPEFRFVDAVDELDIASHIRARYRVRGDEP
                     FLAGHFPGNPVFPGVLQLEALAQAGAVALLSEPRYTGRLPLFGGIEECRFRRIVRPGD
                     ELVLTVTLERLSARGGWARGLATVDDQESCRARLFFALA"
     gene            4225..4491
                     /locus_tag="VFX21_02050"
     CDS             4225..4491
                     /locus_tag="VFX21_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEX5094761.1"
                     /translation="MASPQPQHHGETLTVPERVVIAPAVGVFRPTIVDTGKVVREGDE
                     IGVIEALGTSHPVRSPFDGTIMGMLAHSGERMRAGQPVAWLRVA"
     gene            4488..5480
                     /locus_tag="VFX21_02055"
     CDS             4488..5480
                     /locus_tag="VFX21_02055"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX5094762.1"
                     /translation="MTALPVGIVGWGTAVPDGRLTNADLEARVDTSDAWIRERTGITE
                     RRIAGDGESTASLGTEAARNALKQAGLEASDIDLLIVATATPEQPIPHTGAFIGDNLG
                     LRCGSFDLAAACAGFTYELIVGSSMVNMGYERVLIVGAETLTRITDPADRTTIILFGD
                     GAGAAVLAPVADGAPALLAWDLGCDGSAAGLLQVPAGGSRRPATAATIAAGDHYLKMQ
                     GQNVFKQAVRAVVASANATLERAGVASSDVDWFVPHQANARIIDAAGARLDIPAERTL
                     VNIERYGNTSSASIPLALFEGVDDGRVQPGDVVLLSGFGAGMTWASALLRWTGQ"
     gene            5477..6223
                     /gene="fabG"
                     /locus_tag="VFX21_02060"
     CDS             5477..6223
                     /gene="fabG"
                     /locus_tag="VFX21_02060"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX5094763.1"
                     /translation="MSVERVAFVTGGSRGIGRATVVALAAAGHPVGFCYSSDEAGAKE
                     TIAAAENAGGKAVSVRADIRNVDDVDAAFDTVAEAFGPATVLVNNAGITRDGLVMRMK
                     DDQWGDVIDTNLTGAFHTIRRATPAMMKARYGRIVNVSSVGGHIGQAGQANYAAAKAG
                     LLGLTRSVARELASRNITCNVVAPGPIVTAMTAEMPEDWRSAAEAAVPLGRFGTAEEC
                     AAVITFLCSEPAGYVTGALVPVDGGLGMGH"
     gene            6279..6524
                     /gene="acpP"
                     /locus_tag="VFX21_02065"
     CDS             6279..6524
                     /gene="acpP"
                     /locus_tag="VFX21_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017319975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX5094764.1"
                     /translation="MDRSDALTTIREVAAEVLSVEPDKVTEEARFKEDLDADSLDLVE
                     LVMGLEERFDIEVPEDDLEGVTTIGQAVDLVLAKVGA"
     gene            6546..>7314
                     /locus_tag="VFX21_02070"
     CDS             6546..>7314
                     /locus_tag="VFX21_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX5094765.1"
                     /translation="MAEDRDHKGRVRVAVTGMGVAAPGGQTLDAFWSTLASGRSTAAT
                     ITRFDPSNLPVRFGCEVLDFDPTVYVGPKEARRMDRVTQLGLSAAIQALNDANLPQVD
                     PARCGVIIGTGVGGFISLEEQTAIYLTKGAARVSPFLIPMMMANATAGSIAMQFGWTG
                     PNMCTATACAASAHAVGEAARLIRDETCDVVMTGGCEACITETAVSAFARMTALSTRN
                     DDPEHASRPFDADRDGFVMGEAGAALLLERWDRAVARG"
BASE COUNT         1234 a         2477 c         2433 g         1170 t
ORIGIN      
        1 atcggtggtc tcggtggcaa ctcgatctgg ctcaccaccg acgacggcaa cgcgtactac
       61 tacgcgcacc tcgacgggtt cgcgggtgag ttcgacgcga acaaccagcg acgcgtggtg
      121 aagggcgaga tcgtcgggta cgtcggcaac accggcaacg cggccggcgg gccgacgcac
      181 acgcacttcg agatccgtcc gcagaacatc ggaccggtga acccctatcc gatgctcaaa
      241 gagatgtgcg cggtgcaggg cggcttcgtc ccgccacccg agcaatcggt ggacgtcatg
      301 ggctcgacga ccacgaccac caccctcccc gtacccggct aggtctgggc taggtcagcg
      361 tcgagaccca cgcgagcgtg tcgtcgctgc gccgcggtcg tccactcgag catccgttcg
      421 atctgtcttg cgtatggtgc cgcgcgcggc catctcttct cgttcgacgt gccacgacaa
      481 ggcactgagt gcaccgatgg agccgttcac atgaccgcgc gcgatcaggt cacggatttc
      541 gagttcgccc tggatgccga cgaaccggga ccgtccctac ggtgcgcccg cgcacgtctc
      601 gagcatggtg cggagcgcgt tccactcggc tcgtgtcgcg ctcaggtccc agcggagctt
      661 gatgcgcacc catgcgctcg cgtacacgca ccaggacgac tggtcgggcg gcttccatgc
      721 atcgggtccc ttgtcgctct tcgcggcgtt ctcggcggac gcgagcgcgt tgaggatcat
      781 ggggtcggtg agatcgttcg cgaactgttc ccgccgggcg tcggtccacg tcgacgcgcc
      841 ggagcgccac gcgtcggcga gggcaactgt gtggtcgatc tcgacctcgc cggggtccgt
      901 gtacgagcgg cccgaccacg ggtcggtcca cgagccgctt tggaccgcgc atcccgtcat
      961 caccacggga gtcgtcgact cgaccgcgag cacttcctga cgcgtatcga gacagtcgcc
     1021 gtccgcatcg atccacccgc cgccgaagcg atcccggtcg tacggagtcg agaggtcggg
     1081 ttcggcgacg tgcagggcgt cgagcgtgcc cggcacggtc ggctcctcgg ccgggctctt
     1141 gccggagtcg agcgcgcgca cgagcaagac cgcgacgacg atcgcgacga caacaccaac
     1201 ccacacgagc cgacgccccc gcacggtgcc cagcttgccg gatcaggcgg ttccggtgcc
     1261 ggaatgccca cgacccggtg agccctcgtc gaacggtcgc catcgtcgcg gaaagatcgg
     1321 cgtagcggca gcgcggtccc gggtaacact ccgacggacc gtttgtctgc gcgcggcaac
     1381 gtgtcaggtc gaacgggagg tgatgagtgc ggttgcgcaa tggtcgttgg gaatgcgtga
     1441 actgcgacgc tccgctcccg gtcgacgcca tagacgtcgg cgcggacttc gcggtgaaga
     1501 cggtgatcga ggcttcggcc ggtcgcgcga gcgtgcgcat cctgatggtc aacgggcaag
     1561 aggtacaccg gtgcctcgtc gccgaccatc gagactttct cccggcagcc gaaccctcga
     1621 aggacagcgc ggcggttcac gcgcgcgacg cgacctgggc gtgacgggga gcactcacgt
     1681 ggttctcccc cggtgggatt gaacctgctt gggcgttcgt cgtttcgctc ggattgcgcg
     1741 cgggcgcgtc gggcggtcga gggtcggtgt gatgcgaagt cattgcacgg gttcaatgga
     1801 cgcgagcttc cccctttcct ttgacgggcc gccgccgtca cctggcagca tggctctcgt
     1861 gcctcttccg ttcgccccga tggtcgaatg cctcgcaccg aacgggaccg tgtggtgcac
     1921 cgcgccggta ctccatggct cggtgcgaga cgcgctcgag gcgtgtgcgg aacatgatgt
     1981 cgagggcgtc gtcgcgaaac gactcgggtc tccgtatcgg cgtggaggcg gcgatggctg
     2041 atctcgtgat tcacgccggc atgcccaaga cgggatcgtc tgcggtgcag cggtggctga
     2101 agtcgaacct cgacgcgatt gccgccaatg ggtttcaggt ccgacggcga cctcgtggtc
     2161 tcgacgaggc gacttgcctc gacgaggtga cgcgccccgg ttcgcatata ggagtgaaga
     2221 gctacgtcga cgaccttctc ggtaacgcgc aggagcagcg ggcgctgctt gacgaccttc
     2281 acgcgcttcc cggtaccgcg ctgttgacga gcgaagcgct caacaacctc tttcaccggg
     2341 acgccgtgtg gttgtcggcg ctgaatgact atgccgcgat ccatgacgtc cgcgtcgcat
     2401 actacgtgcg tccacaacac gacgcgatgg aagcatcgtg gcgacagtgg ggcttccgaa
     2461 gacccatgcg gccgtccgta tacctcgaac ggcgtgaagg ccgccgcctc gacttccttt
     2521 cgacgctgat gcgtgtgcgc gaatgtgcgc cgaacgtgtc gttcgaaatg cgcggatttc
     2581 gtcgcgatct cctcgtcggt ggagacatcg tcgtcgactt cgcggacacc ttcctcggta
     2641 tgcacgccga gcccgtcccc tcggtgaacg aaggactacc ccttttcata tgtgtcttga
     2701 tccaggctcg cggcgcgtcc ccgctgtggg agagtcatca tgagaacgcg acgctgcgac
     2761 gcgtgcagga gatcgtctca cgctgggaca tgcccgaacc cgaggacatt caggaatcac
     2821 tgctcgtgct gcaacgccat tgccgatcgc gatacgaaga cgacaatcgc aagttgatct
     2881 cgttgctcaa ctggccgacg gaggattacg tccctccaac caaagaagcg ggcgacgatc
     2941 gcctgtcgcg cctcgacgtt ctctggcggc cgcgaccgga aggccacctg ctgcgacagg
     3001 cgctcgccga catcatcgcc acctcggaca cacgagacgc gcgacagtct cgaccttcat
     3061 ccgatcgcgc gggcgaacgc gtgaccctcc gttccaaact gcgcacagcg acgcgccacg
     3121 gcgccaaggc gctgcgtcgt tcccgaggta aaccatccgc gtagcgggca ggtcgccatt
     3181 ctgaaagtcg tgccgagcaa cggcgttgag agaccttggt acagcgcgga accgtgatca
     3241 cggagcagga cagactctcg ccacggcgtc atctgcgtcc gtcagcagca gtgcccgagg
     3301 tgggatttga acccacacgc cgttgccggc agcggatttt gagtccgccg tgtctgccat
     3361 tccaccactc gggcgtcgga tgaagtctgc catcttgcca ccgatacgat cggagcgtgg
     3421 tcgagttcgt cgtggggctc gtcgtcggtg cgtcggcggc ggtgctgctg ctgatcgtgt
     3481 tcggcgggtc gcggcgcatc cgcgccgaga aaccgctcga acgcgacgtc gagacgcaga
     3541 tcttgctcgg cgaagacccc gacaagacga cgacaccctc ggcgtcaccc agcaagaacc
     3601 acccgcgcaa ctacagcgcc gacgagctcg cccaactgcg gcgactcggt acctcgaggc
     3661 gcaagaaccg ctgactacgc gagcgcgaag aacaagcgcg cgcgacacga ctcctggtcg
     3721 tcgacggtgg cgagcccccg tgcccatccg ccgcgggcgc tcaatcgctc gagcgtgacc
     3781 gtgagtacca actcgtcgcc gggacgaacg atgcgccgga accggcactc ttcgatgcca
     3841 ccgaacaacg gcaaccggcc cgtgtaacgc ggttcggaga gcaacgcgac tgcgcccgcc
     3901 tgcgccagcg cttcgagttg caacacgccc gggaacacgg gattgcccgg gaagtgaccg
     3961 gcgaggaacg gttcgtcgcc gcggacgcga tagcgcgcgc ggatgtgcga cgcgatgtcg
     4021 agctcgtcga ccgcgtcgac gaaccggaac tccggtccgt gcgggagtcg cgcgagcgct
     4081 tcggcatact ggtcgctcat cgccgcgcac gttactcgtc ggtagctcgc gacacgcgat
     4141 gttcaccacg cgcgaacaag caggtcacgg ggatgttcga gcatctgcgt aaccaattgc
     4201 acgcgcgccg agtcagagga cctaatggct tccccccagc cccaacacca cggcgagacg
     4261 ctgaccgttc cggagcgtgt cgtcatcgcc cccgccgtcg gcgtgttccg ccccaccatc
     4321 gtcgacaccg gcaaggtggt gcgcgaaggt gacgagatcg gcgtcatcga agcccttggc
     4381 acttcgcacc cggttcgttc gccgttcgac ggcacgatca tgggaatgct cgcccactcg
     4441 ggtgagcgca tgcgcgccgg gcaaccggtc gcgtggttgc gcgtggcgtg accgcgctcc
     4501 cggtcggcat cgtcggatgg ggcacagcag ttcccgacgg tcgcctgacg aacgcagatc
     4561 tcgaagcgcg cgtcgacacc agcgacgcgt ggattcgcga gcgcacggga atcaccgaac
     4621 gtcgcatcgc gggtgacggc gagtcgacgg cgtcgctcgg caccgaagct gcgcgcaacg
     4681 cgttgaagca agcgggcctc gaagcatccg acatcgacct gttgatcgtc gcaacggcga
     4741 cgcccgaaca gccgatcccc cacaccggcg cgttcatcgg cgacaacctc ggtctgcggt
     4801 gcgggtcgtt cgacctcgcc gccgcgtgcg ccgggttcac ctacgagctc atcgtcggat
     4861 cgtcgatggt gaacatgggg tacgagcgcg tactgatcgt cggcgccgag acgctgaccc
     4921 gcatcaccga tcccgccgac cgcacgacga tcatcctctt cggtgacgga gcgggcgctg
     4981 cggtgctcgc accggtcgcg gacggcgcgc cggccctgct ggcctgggat ctcggctgtg
     5041 acgggtcggc cgcgggactg ctccaagtgc ccgccggcgg cagccgccgc ccggccaccg
     5101 cggcgacgat cgcggcgggc gaccactacc tcaagatgca gggtcagaac gtgttcaagc
     5161 aggccgtgcg ggccgtcgtc gcctcggcca atgccaccct cgagcgggcg ggcgtggcat
     5221 cctcggacgt tgactggttc gtcccccacc aggcgaacgc ccgcatcatc gacgcggcgg
     5281 gcgcccggct cgacatcccg gcggagcgca cgcttgtgaa catcgaacgg tacggcaaca
     5341 cgtcgtcggc atcgatcccg ctcgcgttgt tcgaaggcgt cgacgacgga cgcgtgcaac
     5401 ccggtgacgt cgtcttgctc tcgggtttcg gcgcgggcat gacctgggcg agcgcgctct
     5461 tgcgctggac cggtcagtga gcgtcgagcg cgtcgcgttc gtcacgggcg gatcgcgagg
     5521 tatcgggcgg gccacagtcg tcgcactcgc cgccgccggc catccggtcg ggttctgcta
     5581 cagcagcgac gaagccggtg cgaaggagac gatcgcggcc gcggagaacg cgggcgggaa
     5641 ggccgtctcg gtacgcgccg acatccgcaa cgtcgacgac gtcgacgccg cgttcgacac
     5701 cgtcgcggag gcgttcggtc cggcgaccgt gctcgtgaac aacgccggca tcacccgcga
     5761 cgggctcgtc atgcgcatga aggacgacca gtggggcgac gtcatcgaca ccaatctgac
     5821 gggcgcgttc cacacgattc gacgcgccac gcccgcgatg atgaaggcgc gctacggccg
     5881 gatcgtcaac gtctcgtcgg tcggcggcca catcggccag gccggccaag ccaactacgc
     5941 ggcagccaaa gcgggtctgc tcgggctcac tcgcagcgtc gcccgggaac tggcatcccg
     6001 caacatcacc tgcaacgtgg tcgcgcccgg ccctatcgtc accgcaatga cggcggagat
     6061 gcccgaagac tggcggtcgg cggccgaggc cgcagttccg ctcggccggt tcgggaccgc
     6121 ggaagaatgt gcagctgtga tcacgttcct gtgctcggag cccgccggtt acgtgacggg
     6181 cgcgctggtg cccgtcgacg gcggcctcgg catgggtcac tgaccatgtg ccccgacccc
     6241 acacgtcact gacaaagaca acaaggagaa cgctcgttat ggaccgcagc gacgcgctga
     6301 cgacgatccg ggaagtcgcc gctgaagtgc tcagcgtcga gcccgacaag gtcaccgaag
     6361 aagctcgatt caaggaagac ctcgacgccg acagcctcga cctcgtcgag ctggtgatgg
     6421 gtctcgagga gcgcttcgac atcgaagtgc ccgaagacga cctcgaaggt gtcaccacga
     6481 tcggccaggc cgtcgacctc gtgctcgcca aggtcggcgc gtagccaccg cgtagaggag
     6541 ccatcgtggc cgaagaccgt gatcacaaag ggcgcgtgcg cgtcgccgtc accggcatgg
     6601 gtgtcgccgc gcccggcggg cagaccctcg acgcgttctg gagcacgctc gcgtccgggc
     6661 gttccaccgc ggcgacaatc acgcgcttcg accccagcaa cctcccggtg cgcttcggct
     6721 gcgaggtgct cgacttcgat cccaccgtgt acgtcggccc gaaggaagcg cggcgcatgg
     6781 accgcgtcac gcagctcggc ctctccgccg cgatacaagc gttgaacgac gcgaacctgc
     6841 cgcaggtcga tcccgctcgg tgcggcgtga tcatcggcac gggagtcggc ggtttcatct
     6901 ccctcgaaga gcagaccgcg atctacctca ccaagggcgc agcacgcgtc agcccgttcc
     6961 tcatcccgat gatgatggcg aacgccaccg ccggttcgat tgcgatgcag ttcggctgga
     7021 ccggtcccaa catgtgcacc gcgactgcgt gtgccgccag tgcgcacgca gtcggggagg
     7081 ccgctcggtt gatccgcgac gagacgtgcg atgtcgtgat gacgggcggc tgcgaggcat
     7141 gtatcaccga aacggccgtc tccgcgttcg cgcgcatgac cgcgctctcg acgcgcaacg
     7201 acgaccctga gcacgcatca cgtccgttcg acgccgaccg cgacgggttc gtcatgggcg
     7261 aagccggcgc cgcattgttg ttggaacgct gggatcgagc ggtcgcgcgc ggcg
//
LOCUS       DASUGA010000109         3725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_192264, whole genome shotgun sequence.
ACCESSION   DASUGA010000109 DASUGA010000000
VERSION     DASUGA010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192264"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..666
                     /locus_tag="VFX21_02075"
     CDS             22..666
                     /locus_tag="VFX21_02075"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5094766.1"
                     /translation="MHDTDVAPPRRANDDRGRKPIRGRSGIGTAAALLLWPDDPPGTR
                     EIARRCGLSPSSVSEALRSLRGASLVRSDNRPLIPEAFWALADVWNPPSLALARAPDV
                     DGLVVGGTVAALAWGAPLVVTADYPPDYYTREDVDLRALRYEYGAPAEADTRACTVTV
                     APTQLLLSEYVERPGAAHPLVHPLFAALDLARDKARGVEALEVWSPPPPYKRLW"
     gene            663..1427
                     /locus_tag="VFX21_02080"
     CDS             663..1427
                     /locus_tag="VFX21_02080"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEX5094767.1"
                     /translation="MTGQSALLTGPDAARLVQAAARITSIPELRLAIIGGVAVACRVG
                     TLFRATTDVDAVSAELAYSTDSVASSADLLVVRDIATRVPRGAATDLSVDGTTVQIID
                     TEPIPPDVRDIEPALSRLFVQAHRFALDSAEPMPVVVHDPEVEARIPVAITVALIATK
                     LHALADRHDDAKKASDAHDLMRLLGLVRSSGGPDRLAEAPGLAETIADVMDDVLVENA
                     ERAVRYMHVYGDHGLPQFDAEEVRLRASSFVRMIRS"
     gene            complement(1448..2056)
                     /locus_tag="VFX21_02085"
     CDS             complement(1448..2056)
                     /locus_tag="VFX21_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX5094768.1"
                     /translation="MTRKLVVDAFVSLDGVMQAPGGPDEDTTSGFPYGGWQFGYGDEA
                     VGEYITAFTDKPYDLLLGRTTYEIFNSYWPHQEGPMADGLNTAHHYVASRTLRSAPWK
                     ESTIIRDVPTEVAKLKQEDGPEIQTWGSTILLQTLWEHDLVDAINVLTYPVTLGNKWK
                     HLFGDGTIAAGFKVVDSQISPTGIIMARYERAGDVKIGSFDQ"
     gene            2110..2571
                     /locus_tag="VFX21_02090"
     CDS             2110..2571
                     /locus_tag="VFX21_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5094769.1"
                     /translation="MHGSVTVHMNAPAEKVWDLVSDVTQIGRFSPETFEAEWLDGATG
                     PAVGATFRGHVKRNEKGPKYWTVCKVTECEPNRVFAFGVGNPKKTPLNVWKYELAPAA
                     DGGTDVTESFALQEAIWSKAYWFVLGWARGKRNCGDMQRTLENMKQVVEAA"
     gene            complement(2568..3416)
                     /locus_tag="VFX21_02095"
     CDS             complement(2568..3416)
                     /locus_tag="VFX21_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094770.1"
                     /translation="MKLDLGTFLVQCAAGGMLFCWVTTRRREVSLGYGWLLRIVYGLL
                     AIGGAVALLANDHTVAGAGAILCAAMCAIGLAVSVVRRKAGVGVGAAMMAERKARVAA
                     MVGREDAPVDEPPTGPEFPPVLDLAAGVVGMLTMLACATFAGGPYALAAARLLVGAAF
                     LGSVTDAMLLGHWYLVQPGLRRDPIKELVVWTGAVWPFEILVFLWPVGMVSVLNGTID
                     DGYNGLLGWVWVTCAVTTIGLVVMTWFALKERAYSAVMAATGLLYLAILTAFGVDLIA
                     RAVLTP"
     gene            complement(3425..>3725)
                     /locus_tag="VFX21_02100"
     CDS             complement(3425..>3725)
                     /locus_tag="VFX21_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX5094771.1"
                     /translation="TIGTPCEAVDAAARTVIADAGFGEYFVHRVGHGIGQDAHEDPYM
                     VSGNALPLAAGHAFSVEPGIYFPGRYGMRLEDIVVATTEGPRRLNDAARDLAIVG"
BASE COUNT          630 a         1274 c         1239 g          582 t
ORIGIN      
        1 atcgaggcgc cagggctctt cgtgcacgac acagatgttg cgccgcctcg acgcgcgaac
       61 gacgaccgtg ggcggaagcc gatccggggg cgatccggta tcgggaccgc ggccgcgctg
      121 ttgctttggc cggatgaccc accgggtact cgcgagatcg cgcgacggtg cggtttgtcg
      181 cccagcagcg tgagcgaggc gcttcggtcg ctacgcggcg catcactcgt tcgcagcgac
      241 aaccgccccc taatacctga ggccttttgg gcgctcgccg acgtgtggaa tccaccatca
      301 ctcgcacttg cgcgcgcacc cgacgtcgac ggcctcgtcg tcggcggaac tgttgccgcg
      361 ttggcgtggg gcgcgcctct cgtcgtgacc gcggactacc cacccgatta ctacacgcgc
      421 gaggacgtcg acctgcgggc cctgcggtac gagtacggtg caccggcgga ggccgacacg
      481 cgtgcgtgta cggtcacggt cgctccaacg cagttgctgt tgtccgaata cgtagaacgt
      541 cccggagcag cgcacccgct cgtacacccg ttgttcgcgg cgctcgacct cgcacgcgac
      601 aaggcacgcg gcgtagaagc gctcgaggtc tggtcgcctc ccccgccgta caagcgactg
      661 tggtgaccgg acaatccgcg ttgctgacgg ggccggacgc tgcccgcctc gtccaggccg
      721 cggcgcggat cacctcgata cccgagctcc gactcgcgat catcggcggt gtcgcagtgg
      781 catgccgcgt tggcacactc tttcgagcga cgaccgatgt ggatgccgtc agcgcagaac
      841 tggcttactc caccgactcc gtggcttcga gcgcggatct cctcgttgtg cgcgatatcg
      901 cgacacgcgt cccacggggg gcggcgactg acctctcggt cgacggcacg acggtacaga
      961 tcatcgatac ggaaccgata ccgcccgatg tgcgcgacat cgaacccgcg ctctctcggt
     1021 tattcgtgca ggcgcaccga ttcgcgctcg actccgcaga gccgatgccc gtcgtcgtcc
     1081 acgaccccga ggtcgaggcg cggattcccg tggcgatcac cgtcgcgctc atagcgacga
     1141 agctgcacgc gctcgcggat cgacacgacg acgcaaagaa ggcgtcagat gcgcacgatc
     1201 tcatgcgatt gctcgggctc gtgcggtcat ccggtggacc cgaccggctg gccgaggcgc
     1261 cgggcctcgc cgagacgatc gccgatgtaa tggacgatgt gctcgtcgag aatgcggaac
     1321 gagccgttcg gtacatgcac gtgtacggcg accacgggct tccccagttc gacgcagagg
     1381 aggtacgtct ccgggcaagc agcttcgtgc gaatgattcg ctcgtagcct cgcgtcaccg
     1441 tcgggcgcta ctggtcgaag gaaccgatct tcacgtcgcc cgcgcgttcg tagcgcgcca
     1501 tgatgatccc agtcggggag atctggctgt cgacgacttt gaagccggcc gcgatcgtgc
     1561 cgtcgccgaa caggtgcttc cacttgttgc ccaacgtcac ggggtacgtc agcacgttga
     1621 tcgcgtcgac gaggtcgtgc tcccagagcg tctgcaacaa gatggtgctg ccccaggtct
     1681 ggatctcggg cccgtcctcc tgcttcagct tcgcgacttc ggtcgggacg tcgcgaatga
     1741 tcgtcgactc cttccacggt gccgaccgca acgtgcgcga cgcgacgtag tgatgcgcgg
     1801 tgttgagacc gtcggccatc gggccctctt ggtgcggcca gtacgagttg aagatctcgt
     1861 acgtcgtgcg cccgaggagc aggtcgtacg gcttgtcggt gaacgcggtg atgtattcac
     1921 cgacggcttc gtctccgtag ccgaactgcc acccgccgta cgggaacccg ctcgtcgtgt
     1981 cttcgtcagg accgccgggc gcctgcatca cgccgtcgag tgaaacgaat gcgtctacta
     2041 ccagtttgcg tgtcatgccg tatacgacct ccccgcggcc cgaaactcat cgcgcgtaat
     2101 gttccccgca tgcacggctc cgtcacggtc cacatgaacg cgccggccga gaaggtctgg
     2161 gacctcgtga gcgatgtcac gcagatcggc cgcttcagcc ccgagacgtt cgaagccgaa
     2221 tggctcgacg gcgccaccgg cccggccgtc ggcgcgacct tccgcggaca cgtgaagcgc
     2281 aacgagaagg gcccgaagta ctggacggtg tgcaaggtca ccgagtgcga accgaaccgc
     2341 gtgttcgcgt tcggtgtcgg gaacccgaag aagactccgc tcaacgtgtg gaagtacgag
     2401 ctcgcgccgg cggccgacgg cggcaccgac gtcacggagt cgttcgcgct gcaagaggcg
     2461 atctggtcga aggcctactg gttcgtgttg ggctgggctc gcggcaagcg caactgcggc
     2521 gacatgcagc gcacgctgga gaacatgaag caggtcgtag aagcggccta gggcgtgagc
     2581 acggcgcgcg cgatcaggtc gacgccgaac gcggtgagga tcgcgaggta cagcagcccg
     2641 gtcgcggcca tgaccgcgga gtacgcgcgc tccttcagtg cgaaccacgt catcacgacg
     2701 agaccgatgg ttgtgaccgc gcacgtgacc cagacccagc cgagcagccc gttgtaaccg
     2761 tcgtcgatgg tgccgttcaa cacggacacc atgccgaccg gccacaggaa gacgaggatc
     2821 tcgaacggcc acaccgcgcc ggtccacacg accagctcct tgatcggatc gcggcgcaaa
     2881 cccggctgca cgagatacca gtgcccgagc agcatcgcgt cggtgacgga cccgaggaac
     2941 gcggcgccga cgagcagccg cgccgcggcg agcgcatacg gaccgcccgc gaacgtcgcg
     3001 caggccagca tcgtcagcat tccgacgaca ccggccgcga gatcgagcac cggcgggaac
     3061 tcgggaccgg tcggcggttc gtcgacgggt gcgtcctcgc ggccgaccat cgccgcgacg
     3121 cgcgccttgc gctcggccat catcgccgcg cctacgccga cgcccgcctt gcgccgcacg
     3181 accgagaccg cgagtccgat cgcgcacatc gcggcgcaga ggatcgcgcc cgcgccggca
     3241 acggtgtggt cgttcgcgag cagcgcaacc gcaccaccga tcgcgagcag gccgtacacg
     3301 atgcgcaaga gccagccata gccgaggctg acttcgcggc ggcgggtcgt cacccagcag
     3361 aacaacatgc cgcccgcggc gcactgcacg aggaaggtcc cgaggtcgag cttcacggtc
     3421 cgcctcagcc gacgatcgcg agatcgcgcg cggcgtcgtt gagccggcgc ggtccctcgg
     3481 tcgtcgcgac gacgatgtct tcgagccgca taccgtaccg gccggggaag tagatcccgg
     3541 gttcgacgct gaacgcgtgg ccggccgcga gcggcagcgc gttgcccgac accatgtacg
     3601 ggtcctcgtg cgcgtcctgg ccgatgccgt ggccgacacg gtgcacgaag tactcgccga
     3661 agcccgcgtc cgcgatcacg gtgcgggcgg cggcgtcgac ggcttcgcac ggcgtgccga
     3721 tcgtc
//
LOCUS       DASUGA010000110         2127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_607485, whole genome shotgun sequence.
ACCESSION   DASUGA010000110 DASUGA010000000
VERSION     DASUGA010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607485"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..959
                     /locus_tag="VFX21_02105"
     CDS             153..959
                     /locus_tag="VFX21_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094772.1"
                     /translation="MPDQGALEAERDFLLRSLDDLELERQAGNVDDDTYRTLHDDYTA
                     RAASVIRALRDDSLPEPVDVRPPGRRWMRVATGAGIVVFAIAAAVLLANTVGSRNDDQ
                     TITGNDANAQQSIDDLAAAAKANPDSYPAQIAYARATLSTDPATSIAQFDAAGRLDPS
                     QPEPPTYIGWISKLTADTLPAGADRDALTDRAISEFDRAISIDPTYADAYVFRALAKR
                     DLLGDDAGAATDFQLFLVNAPDDHPMRSVVLDALAEVSPSSTTVTPSTTP"
     gene            986..1459
                     /locus_tag="VFX21_02110"
     CDS             986..1459
                     /locus_tag="VFX21_02110"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5094773.1"
                     /translation="MAKQPAPQTDASKIYRTTITTNRGTIVMDLDPQLAPNTVNNFVG
                     LARDGYYDGLSFHRVVPEFVIQGGCPEGSGRGGPGYKFDDEPVQGEYTLGAVAMANAG
                     PNTNGSQFFICIDDCRRKLTPNYNLFGYVKEGMDVALATQVGDVMQTVKIEEVDR"
     gene            complement(1537..>2127)
                     /locus_tag="VFX21_02115"
     CDS             complement(1537..>2127)
                     /locus_tag="VFX21_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013808660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5094774.1"
                     /translation="GVKGFTLALIIGGALGLAVSRSRILRSAIGSMITGLQTMPSVAW
                     FPLAILLFKATEEAIMFVVVLGAAPSIANGLIQGVDQIPPILLRAGRVLGARGSSAVR
                     HVMLPAALPSFVGGLKQGWAFAWRSLMAGELLTLIPGKVSLGAKLEFERRFSDSAGML
                     AVMVMIFLIGVTIDALVFGQADRFIRRRYGLIDAAH"
BASE COUNT          381 a          747 c          701 g          298 t
ORIGIN      
        1 gctcatcgcg tgggggctac ccgtgatcgc gatcatcctc ggcgcggccg gcatcgcgtt
       61 cgcactgcgg cggtggtcgt cgcaaccgcg gctcgcggcc accgccgacg acgaggtcga
      121 ggtcgagcgc gcccgcagaa cggcgaccgg cgatgcctga ccagggcgcg ctcgaagccg
      181 aacgcgactt cctcctccgt tccctcgacg acctcgagct ggagcggcag gccggcaacg
      241 tcgacgacga cacgtatcgc acgttgcacg acgactacac cgcgcgggcg gcgtcggtga
      301 tccgggcctt gcgcgacgac tcgttgccgg aacccgtcga cgtccggccg ccgggcaggc
      361 gatggatgcg cgtcgccacc ggcgccggca tcgtcgtgtt cgcgatcgcg gcggcggtgt
      421 tgctcgccaa cacggtcggc tcccggaacg acgatcagac gatcaccggg aacgacgcga
      481 atgcgcaaca gtcgatcgac gacctggccg cggcggccaa ggccaatccc gacagctacc
      541 ccgcccagat cgcgtacgcg cgcgcgacgc tctcgaccga cccggcgacg tcgatcgcgc
      601 aattcgacgc ggccggtcgc ctcgacccgt cgcaaccgga accgccgacg tacatcggct
      661 ggatcagcaa gctgacggct gacacccttc cggccggcgc cgaccgtgac gcgctcaccg
      721 atcgcgcgat ctccgagttc gatcgcgcga tctcgatcga tcccacgtac gccgacgcgt
      781 atgtcttccg ggcgctggcc aagcgtgatc tgctcgggga cgacgcgggc gcggcgaccg
      841 acttccagct gttcttggtc aacgcgcccg acgaccaccc gatgcggtcg gtcgtgctcg
      901 acgcactcgc cgaagtgtcg ccgtcgagca ctactgtgac gccttccaca accccctgac
      961 atcgagcccc gactgggagg ccctcgtggc aaagcaacca gccccgcaga ccgacgcgtc
     1021 gaagatctac cggacgacga tcacgacgaa ccggggcacg atcgtgatgg acctcgaccc
     1081 gcagctcgcg ccgaacacgg tgaacaactt cgtcggcctc gcacgcgacg gttactacga
     1141 cgggctgtcg tttcaccgtg tcgttcccga gttcgtgatc cagggcggct gtcccgaggg
     1201 atccgggcgg ggcgggccgg gctacaagtt cgacgacgag cccgtgcagg gcgagtacac
     1261 gctcggcgcc gtcgcgatgg cgaacgcggg cccgaacacg aacggctcgc agttcttcat
     1321 ctgcatcgac gactgccgcc gcaagctcac cccgaactac aacctgttcg gctatgtcaa
     1381 agaaggcatg gatgtcgcgc tcgcgaccca agtcggcgac gtcatgcaga ccgtgaagat
     1441 cgaagaggtc gaccggtaga gctcgggcgc gtgcggttct cggtcatccc gaccccggat
     1501 gtgcgggccg ggatgaccga gaatgatcgt cgcgactcag tgcgcggcgt cgatcagtcc
     1561 gtagcggcgg cggatgaagc ggtcggcctg gccgaagacg agtgcgtcga tcgtgacgcc
     1621 gatgaggaag atcatcacca tgacggccaa catgccggcc gagtcgctga accgacgttc
     1681 gaactcgagc ttggcgccga gcgacacctt ccccgggatg agcgtcaaga gctcacccgc
     1741 catcagcgat cgccacgcga acgcccagcc ttgcttcagg ccgccgacga acgacggaag
     1801 cgccgccggc agcatcacgt gccgtaccgc ggaggatccg cgcgcgccga gcacgcgtcc
     1861 ggcgcgcagc aggatcggtg ggatctggtc gacgccctgg atcagaccgt tcgcgatcga
     1921 cggcgccgcg ccgaggacga cgacgaacat gatcgcttcc tcggtcgctt tgaacagcag
     1981 gatcgcgagc gggaaccacg cgaccgacgg catcgtctgc agcccggtga tcatcgaacc
     2041 gatcgcggac cggaggatgc gtgagcgtga cacggcgagc ccgagcgcgc cgccgatgat
     2101 caatgcgagc gtgaaaccct tgacccc
//
LOCUS       DASUGA010000111         2469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_903650, whole genome shotgun sequence.
ACCESSION   DASUGA010000111 DASUGA010000000
VERSION     DASUGA010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903650"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..1404
                     /gene="leuC"
                     /locus_tag="VFX21_02120"
     CDS             7..1404
                     /gene="leuC"
                     /locus_tag="VFX21_02120"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX5094775.1"
                     /translation="MAQTLSEKVWDRHVVRTAAGEPDLLFIDLHLVHEVTSPQAFDGL
                     RMAGRGVRRPDLTIATMDHNVPTAGLNRPLEDPISAKQMEVLAKNCDEFGIRCYGMRD
                     PGQGIVHVIGPEQGLTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLPQ
                     MKPGTMAITVEGDAPLGVTAKDIVLAIIARIGTGGGIGSVLEYRGPAIRALSMEGRMT
                     VCNMSIEAGARAGLIAPDDTTFAYLEGRPFAPKGAQWEAALDDWRSLVTDAGAPFDKE
                     VVIDATTLVPHVSWGTNPAQTIRIDATVPDPDSYADPSDQEAARRALAYMGLQAGTPM
                     RDVAVDTVFIGSCTNSRIEDLRAAAAVARGHKVKPGVRTLVVPGSYAVKAQAEAEGLD
                     QVFTAAGFDWREPGCSMCLAMNPDKLAPGERCASTSNRNFEGRQGKGGRTHLVSPAVA
                     AATAIAGHFATPEDL"
     gene            1405..1998
                     /gene="leuD"
                     /locus_tag="VFX21_02125"
     CDS             1405..1998
                     /gene="leuD"
                     /locus_tag="VFX21_02125"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX5094776.1"
                     /translation="MDAVRVITGRGVPLVRSDVDTDQIIPSDWLKRVERTGFGAGLFS
                     EWRENSDFVLNQERYDGANILVAGQNFGTGSSREHAGWALMDYGFAAVLSPRFADIFR
                     NNCGKIGLVAATVDQSVCTKLWEAIEEDPSLEIVVDVEHKRVAAPAIGLDEPFVLDDF
                     TQYRLVNGLDDIGLTLRHEAHIATHETKRAAHLPTVS"
     gene            complement(1991..>2469)
                     /locus_tag="VFX21_02130"
     CDS             complement(1991..>2469)
                     /locus_tag="VFX21_02130"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006631367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5094777.1"
                     /translation="LVLSAYPEARGFGLDFQEEMLRRARERFDGDDRVELRAHNMDES
                     LPADLSGFDAVVSSFAIHHLAPVRQRALYAEVFERLHPGGIFVNVEHVASRSDELHVQ
                     FLASVGRAPEDDDPSNQLVTVETQLDWLDGIGYEHADCTWKWRELAVLAAWKPPVS"
BASE COUNT          412 a          859 c          843 g          355 t
ORIGIN      
        1 gcaacgatgg cgcagaccct ctccgagaag gtctgggacc ggcacgtggt ccgcaccgcg
       61 gccggtgaac ccgacctgct gttcatcgac ctgcacctcg tgcacgaggt gacgtcgccg
      121 caggccttcg acggcctgcg catggccggg cggggcgtgc gccgccccga cctgacgatc
      181 gcgacgatgg accacaacgt cccgaccgcg ggactcaatc gcccgctcga ggacccgatc
      241 tcggccaagc agatggaagt cctcgcgaag aactgcgacg agttcggcat ccgctgctac
      301 gggatgcgcg acccggggca gggcatcgtg cacgtcatcg gccctgaaca aggcctgacg
      361 caaccgggca tgacgatcgt gtgcggcgac agccacacct cgacgcacgg cgcgttcggt
      421 gcgctcgcgt tcggcatcgg caccagcgag gtcgagcacg tgctcgcgac gcagacgctg
      481 ccgcagatga agccgggcac gatggcgatc accgtcgagg gcgacgcgcc gctcggtgtc
      541 accgcgaagg acatcgtgct cgcgatcatc gctcgcatcg gcaccggcgg cggcatcggt
      601 tcggtgttgg agtaccgcgg tcccgcgatt cgcgcgctgt cgatggaagg ccgcatgacg
      661 gtctgcaaca tgtcgatcga agcgggcgcg cgtgccggtc tgattgcgcc ggacgacacg
      721 acgttcgcgt acctcgaagg gcggccgttc gcgcccaagg gcgcgcagtg ggaggcggcg
      781 ctcgacgact ggcgttcgct cgtcaccgac gccggcgcgc cgttcgacaa ggaagtcgtg
      841 atcgacgcga cgacgttggt gccgcacgtg tcgtggggca ccaaccccgc gcagacgatc
      901 cgcatcgacg ccaccgtgcc cgaccccgac tcctacgccg acccgagcga ccaggaagcg
      961 gcccggcgcg cactcgcgta catgggcctg caggccggca cgccgatgcg cgacgtcgcg
     1021 gtcgacaccg tgttcatcgg gtcgtgcacc aattcccgca tcgaagacct gcgcgccgcg
     1081 gccgcagtcg cccgcggtca caaggtgaag ccgggcgtgc gcacgctcgt cgtccccggt
     1141 tcctacgcgg tgaaggcgca ggccgaagca gaaggtctcg accaggtgtt cacggcggcc
     1201 ggcttcgact ggcgcgagcc ggggtgctcg atgtgcctcg cgatgaaccc cgacaagctc
     1261 gcgccgggcg agcggtgcgc gtcgacgtcg aaccgcaact tcgaaggccg ccaaggcaag
     1321 ggcgggcgca cacacctcgt ctcacctgcc gtcgccgccg ccaccgcgat cgcgggccac
     1381 ttcgcaactc cggaggatct gtagatggat gccgtgcgtg tcatcacggg tcggggcgtg
     1441 ccgttggtgc gttccgacgt cgacaccgac cagatcatcc cgagcgattg gttgaagcgc
     1501 gtcgagcgca ccgggttcgg cgcgggcctg ttctccgagt ggcgcgagaa ctccgacttc
     1561 gtgctcaacc aggagcgcta cgacggcgcg aacattctcg tcgcgggcca gaacttcggt
     1621 accggctcgt cgcgggagca cgccggctgg gcattgatgg actacggctt cgccgccgtg
     1681 ctgtcaccac gcttcgccga catcttccga aacaactgcg gaaagatcgg cctcgtcgcc
     1741 gcgacggtcg accagagtgt ctgcaccaag ctgtgggaag cgatcgagga agacccgagt
     1801 ctcgagatcg tcgtcgacgt cgagcacaag cgcgtcgccg cccccgcgat cggcctcgac
     1861 gagccgttcg tgctcgacga cttcacgcag taccggctcg tgaacggcct cgacgacatc
     1921 ggcctgacgc tccgccacga agcccacatc gcgacgcacg agacgaagcg ggcggcacac
     1981 ctcccgaccg tcagctgacg ggcggcttcc atgccgcgag cactgcgagc tcccgccact
     2041 tccacgtgca gtcggcgtgc tcgtagccga tgccgtcgag ccagtcgagt tgggtttcga
     2101 cggtgacgag ctggttcgac gggtcgtcgt cctcgggcgc gcggccgacc gacgcgagga
     2161 actgcacgtg caactcgtca ctgcgtgacg cgacgtgctc gacgttcacg aagatgccgc
     2221 cgggatgcag ccgctcgaag acctcggcgt agagcgcgcg ctgacgcacc ggcgcgaggt
     2281 gatggatcgc gaagctggac accacggcgt cgaacccgga gagatcggcg ggcagcgact
     2341 cgtccatgtt gtgcgcgcgc agctcgacgc ggtcgtcacc gtcgaaccgc tcccgcgccc
     2401 ggcgcagcat ctcctcttgg aagtcgagac cgaacccgcg ggcctccggg tacgcggaca
     2461 gcacgaggg
//
LOCUS       DASUGA010000112         2210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1719889, whole genome shotgun sequence.
ACCESSION   DASUGA010000112 DASUGA010000000
VERSION     DASUGA010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719889"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VFX21_02135"
     CDS             <1..210
                     /locus_tag="VFX21_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094778.1"
                     /translation="IVQLRRQYRDLTDAVRDIRMVLDDSEPDAIDVPDIRRNLDQLAT
                     ELRYLRGRETDLVYEAYTVDLGAGD"
     gene            complement(171..>2210)
                     /locus_tag="VFX21_02140"
     CDS             complement(171..>2210)
                     /locus_tag="VFX21_02140"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEX5094779.1"
                     /translation="LRTGTASLSGLEGEHPVARIACVPAGPYPLAGDIRVGSVWMSDQ
                     WGRVRDVEQRYDFERGELHTAFRFHSDGVTVTVQVVTFCSRSQPSLAAQEVVVVPDHA
                     CEIEIAAGIDPRNIAGRWLERCTTIPGTGDDDVDAAMLWETLGALGSCGCAYWTEAHG
                     PETTRSKSEEAGAPLSTAYRAQTVRKNQRFVVRQVTSMIPSSDHQQPHWHATRLAALG
                     REIGFDSLRKDNAAAWKELWRSRVVLLNADARWQSLADAAFFYLHTSTHPGSFSTHPF
                     GLAQWHGYHYYYGHVMWDVESFLLPPLILTNPDAALAMLEYRMRGMAAARENARLGGF
                     RGLQFPWEASPSKGEEAAPGLGPAAAYEHHVSLDVARAFADYANATGDDVFLTDRAWP
                     VLSGIAEWIVSRATQTRRGWEIHKAMGIAEREKPADNPAFVNMTAVVALRAALRCAAR
                     MGVEPPDAWRSMAEHTVLPLNRNRVIIDHDGYTVREDKASTPAALAGIFPGGYAVDER
                     VERATLEFYLDMADDYAGSPMLSPLLGAWAAMLGDRRRSAALFEEGYAKFSSDRFNIV
                     YEYRPDKFPDEPRSGPFMANMGGFLISCMYGLTGLRIGPGDPSEWCTKPVVMPELWDG
                     VEIERLNVRGREASLHAKHGDAHATLELHEGVSARRHVTTTRAQSPAPRSTVYAS"
BASE COUNT          350 a          753 c          737 g          370 t
ORIGIN      
        1 atcgtccagc tccgtcggca atatcgcgac ctcacggacg cggtgcgcga catccgtatg
       61 gtcctcgacg actccgaacc cgacgcgatc gacgtccccg acattcgccg caacctcgat
      121 cagctcgcca ccgagctccg gtatctgcgg ggccgggaaa ccgatctggt ctacgaggcg
      181 tacaccgtcg acctcggcgc cggagactga gcgcgtgttg tcgtgacgtg tcgtcgcgcc
      241 gagacgccct catggagttc gagcgtcgcg tgcgcatctc cgtgcttggc gtggagtgac
      301 gcttcgcgac cgcgcacatt cagccgctcg atctcgacgc cgtcccacag ctccggcatg
      361 acgacaggct tcgtacacca ctccgacgga tcgccgggcc cgatgcgcag gcccgtgagc
      421 ccgtacatgc agctgatgag gaacccaccc atgttcgcca tgaacgggcc ggatctcggt
      481 tcgtcgggga acttgtcggg ccggtactcg tacacgatgt tgaaccggtc ggagctgaac
      541 ttcgcgtagc cctcttcgaa caacgctgcc gagcgacggc ggtcgcccag catggcggcc
      601 cacgctccga ggagcggcga cagcatggga ctcccggcgt agtcgtcggc catgtccagg
      661 tagaactcga gcgtcgcgcg ttcgacgcgc tcgtccaccg cgtagccgcc gggaaagatc
      721 ccggcgagcg cagccggcgt gctcgccttg tcctcgcgca ccgtgtatcc gtcgtgatcg
      781 atgatcacgc ggttgcgatt cagcggtaac acggtgtgct cggccatgct gcgccacgcg
      841 tccgggggtt cgacacccat ccgtgccgca caccgcagcg cggcgcgcag tgcaacgacg
      901 gcggtcatgt tcacgaacgc tggattgtcg gcgggcttct cgcgttcggc gatgcccatg
      961 gccttgtgga tctcccagcc acgtcgcgtc tgcgtggctc ggctcacgat ccactcggcg
     1021 atcccggaca gcacgggcca cgcgcgatcg gtgaggaaca cgtcgtcgcc ggtcgcgttt
     1081 gcatagtccg cgaatgctct tgcgacgtcg agcgacacgt ggtgctcgta cgcggcggcc
     1141 ggcccgagac cgggcgcggc ttcctcgccc ttcgaggggc tcgcctccca agggaactgc
     1201 agtccccgga acccgccgag gcgtgcgttc tcgcgtgcgg cggccatgcc gcgcatgcgg
     1261 tattccaaca tcgcgagtgc ggcgtccgga ttcgtgagga tgagcggcgg aaggaggaac
     1321 gactcgacat cccacatcac gtgcccgtag tagtagtggt atccgtgcca ttgggcgagg
     1381 ccgaacgggt gggtgctgaa ggacccgggg tgcgtcgacg tatggaggta gaagaacgcc
     1441 gcgtccgcga gtgactgcca tcgcgcgtcg gcattgagca gcacgacgcg actccgccag
     1501 agctccttcc atgccgccgc gttgtccttg cgcagcgagt cgaacccgat ctcgcgaccg
     1561 agcgccgcga gacgggtcgc gtgccagtgc ggctgctgat gatcgctgct cgggatcatt
     1621 gatgtcactt gccggaccac gaaccgctgg ttcttgcgga ccgtctgcgc tcggtacgca
     1681 gtggagagcg gtgcgccggc ctcctcggac ttcgatcggg tggtctcggg accgtgtgcc
     1741 tcggtccagt acgcacagcc gcaggaaccc agggcaccga gcgtctccca gagcatggcg
     1801 gcgtcgacgt cgtcgtcgcc ggttccggga atcgtggtgc atcgctcgag ccatcggccg
     1861 gcgatgttcc gcgggtcgat ccctgcggcg atctcgatct cacacgcgtg gtccggaacc
     1921 acgaccactt cttgcgccgc gagcgacggc tgggaacgcg agcagaacgt cacgacctgc
     1981 accgtgaccg tgacgccatc ggagtggaac cggaacgccg tgtgcagctc accgcgctcg
     2041 aagtcgtagc gctgttcgac gtcgcgcaca cgaccccact ggtcggacat ccacaccgag
     2101 ccgacgcgga tgtcgccggc gagtgggtac ggcccggccg gcacgcaggc gatgcgcgcg
     2161 actggatgct ccccttcgag cccgctcaag gaggcggtcc cggtccgcaa
//
LOCUS       DASUGA010000113         2988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_868178, whole genome shotgun sequence.
ACCESSION   DASUGA010000113 DASUGA010000000
VERSION     DASUGA010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868178"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VFX21_02145"
     CDS             <1..760
                     /locus_tag="VFX21_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017949317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5094780.1"
                     /translation="LQQADIGVAMGKGGTEVAKEAADMILTDDDFATIEAAVEEGRGV
                     FDNLTKFITWTLPTNIGEGCIILVAIMLGATLPILPVQILWINMTTAVALGLMLAFEP
                     TEADVMVRAPRDPRRSLLTGPLLFRLVLVSTLLVGGAWWLFAWERSNGASLAEARTAA
                     LNLFVAVQAFYLFNCRSLTHSMWHVGMLSNRWLLGGLTVQAVLQIAITYLPVMNSMFR
                     TAPIGAETWLAVFAIALGASMVVGLDKRLRQRPM"
     gene            847..1623
                     /locus_tag="VFX21_02150"
     CDS             847..1623
                     /locus_tag="VFX21_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5094781.1"
                     /translation="MQHDARRVVFGDDGSRGADIAWLWINNHPWPGWHVDVLTGADPP
                     YDPSTWGVTPTLEPWTPPWGRTYLNPEKPLAVTFRHADSDPRLVLEEQTDADVVVVGH
                     TGIGHLRSLWLGSTSEWLLHHPTAPLAIIHSAAAVRDVVYCADGSASASKALETFLTL
                     PLAAGTNVTVLSVDDGRADVDAAVSMATTALHAAGIDPHTERVTGKPTPRILEHLQEN
                     QPQLVVLGTKGLTGWQRLRLGSTAAAVVHHVPGATLVACA"
     gene            1915..2151
                     /locus_tag="VFX21_02155"
     CDS             1915..2151
                     /locus_tag="VFX21_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094782.1"
                     /translation="MTSWMASTMDGAGMMYGNGMMSGNGMMGNGMMPGPMMGDVDNMV
                     AACEQWMSSTPAPGATSSSAWCRQMAEWMQERAN"
     gene            complement(2170..>2988)
                     /locus_tag="VFX21_02160"
     CDS             complement(2170..>2988)
                     /locus_tag="VFX21_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013016576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEX5094783.1"
                     /translation="VDYDQDVFEDIKNEFRDFAAKLNVQDLSTIPVSALHGDNVVDRS
                     ANMPWYEGTSLLHHLEEVHIASDRNLIDCRFAVQYVIRPRDDRFHDYRGYAGLVASGT
                     YKAGDDVVVLPSGFESKIASIDTADGPVDEATPPMSVTMRLTDDLDVSRGDMICRPNN
                     RPQVGQDVDAMVCWFSERAKLTPGAKYAIKQTSKWARALVKDLHYRLDVNTLHRDEDA
                     TELTLNEIGRITLRTTQPLFFDEYRRNRSTGSFVLVDEATNDTVAAGMIMGPAS"
BASE COUNT          490 a          971 c         1003 g          524 t
ORIGIN      
        1 gttgcagcaa gcagacattg gtgtcgcgat ggggaagggg ggaacggagg tcgccaagga
       61 agccgccgac atgatcctca ccgacgacga cttcgccacg atagaagccg cggtcgaaga
      121 agggcgcggt gtgttcgaca atctgacgaa gttcatcact tggacgctgc cgaccaacat
      181 cggtgaagga tgcatcattc tcgtcgcgat catgctcggc gcgacgctgc cgatcctgcc
      241 ggtgcagatc ctatggatca acatgacgac cgcggtggcg ctcggattga tgctggcctt
      301 cgaacccacg gaggccgacg tcatggtgcg ggcgccacgc gatccgcgac gctcactgct
      361 gaccggcccg ctcctcttcc ggctcgtgct cgtctcgacg ctcctggtcg gtggggcgtg
      421 gtggctcttc gcgtgggagc gctcgaacgg tgcatcgctt gccgaagcgc gcaccgccgc
      481 gttgaatctg ttcgttgccg tccaggcctt ctacttgttc aactgccgat cactcaccca
      541 ttcgatgtgg cacgtcggca tgctttcgaa tcgctggctt ctcggcggtc tgacggtgca
      601 agcggtccta cagatcgcga tcacctacct cccggtaatg aactcgatgt tccggaccgc
      661 gcccatcggt gcggagacgt ggctcgccgt cttcgcaatc gcgctcggcg cctcgatggt
      721 cgtcggtctc gacaagcggc tccggcagcg cccgatgtag agacgatcgg ccctagtcat
      781 cgcggtcgac aacgccgacg atcgagggag cctgctcgtg cggcgcacag gatcggagac
      841 tgagacatgc aacacgacgc gagacgagtt gtcttcggcg acgacgggtc gcgtggcgcc
      901 gacatcgcgt ggctctggat caacaaccat ccctggcccg gctggcacgt cgacgtgctc
      961 accggggccg acccgccgta cgacccgtcg acctggggcg tgaccccgac cctcgaaccc
     1021 tggaccccac cgtggggccg cacgtacctc aacccggaga aacccctcgc ggtcaccttc
     1081 cggcacgccg actcggatcc gcgcctcgtg ctcgaggaac aaacggacgc cgacgtggtc
     1141 gtcgtcgggc acaccgggat cgggcacctc cggtcgctgt ggctgggcag cacgtccgaa
     1201 tggctgttgc atcacccgac ggcgccgctc gccatcatcc attcggcggc cgcggtgcgc
     1261 gacgtcgtgt actgcgcaga tggctctgcc tccgcgtcga aggcactcga gacgttcctg
     1321 acgttgccac tcgcggccgg caccaacgtg acggtgttgt cggtggacga cggccgggcc
     1381 gacgtcgacg ccgcagtgtc gatggcgacg actgcactgc acgccgccgg catcgatcca
     1441 cacacagagc gggtgaccgg caaaccgacg ccgcgtatcc tcgaacatct gcaggagaac
     1501 cagccgcagc tggtcgtgct cggcacgaag ggcctcaccg gttggcagcg ccttcgcctc
     1561 ggatcgaccg cggccgcagt cgtccaccac gtgcccggcg cgacgctggt cgcgtgcgca
     1621 tgacgcgcgt gtcgggacct tcggctctgt cgtaagacgc gccggtggcg tgtgatggaa
     1681 ccatggcgaa cgaaacacag gaaacacagc ccgtagtcca ccgttcatgg ttgccctggg
     1741 tgctcgtgat gctcggccat cgtcgtcatc gccggtgtca gcgcgctgct cctcgcggac
     1801 cgtgacgacg actcgaatcg caccggcgcg gtaccgctct ccgatgttcg ggagcagtgt
     1861 gcgcagtggc gtgacgcatc ggccggcgcg ccgtccgcgg agtggtgcga tgccatgacg
     1921 tcgtggatgg cctcgacgat ggacggcgcg ggaatgatgt acggcaacgg catgatgagc
     1981 ggcaacggca tgatgggcaa cggcatgatg ccaggcccga tgatgggcga cgtcgacaac
     2041 atggtcgcgg cgtgcgagca gtggatgtcg agcacgccgg cgccgggcgc gacatcatcg
     2101 tcggcctggt gccgccaaat ggccgagtgg atgcaggaac gcgcgaactg aaccggctgc
     2161 cggtccggac tagctcgcgg gtcccatgat catgccggcg gccaccgtgt cgttggttgc
     2221 ttcgtcgacg agcacgaacg aaccggtcga acggttgcgg cggtactcgt cgaagaacaa
     2281 cggctgcgtc gtgcgcaacg tgatgcggcc gatctcgttg agcgtcagct cggtcgcgtc
     2341 ctcgtcgcgg tgcaacgtgt tcacgtcgag gcggtagtgg aggtccttga ccagcgcgcg
     2401 cgcccacttc gacgtctgct tgatcgcgta cttggcgccc ggcgtcagct tcgctcgttc
     2461 gctgaaccaa cacaccatcg cgtcgacgtc ctggccgacc tgcggtcggt tgttcgggcg
     2521 gcagatcatg tcgccccgac tgacgtcgag gtcgtcggtg agtcgcatcg tgaccgacat
     2581 cggcggtgtc gcctcgtcga ccgggccgtc ggcggtgtcg atcgacgcga tcttcgactc
     2641 gaaacccgac ggcagcacga cgacgtcgtc gccggctttg tacgtgccgc tcgcaacgag
     2701 accggcatag ccgcggtagt cgtggaagcg gtcgtcgcgg ggccggatga cgtactgcac
     2761 ggcgaagcgg cagtcgatca ggttgcggtc ggacgcgatg tgcacttctt cgaggtggtg
     2821 caacagcgac gtgccctcgt accacggcat gttcgccgag cggtcgacga cgttgtcgcc
     2881 gtgcaacgcc gacaccggga tcgtcgagag gtcctgcacg ttgagcttcg cggcgaagtc
     2941 gcggaactcg ttcttgatgt cctcgaagac gtcctggtcg tagtcgac
//
LOCUS       DASUGA010000114         3182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1822981, whole genome shotgun sequence.
ACCESSION   DASUGA010000114 DASUGA010000000
VERSION     DASUGA010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822981"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VFX21_02165"
     CDS             complement(<1..334)
                     /locus_tag="VFX21_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010226333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5094784.1"
                     /translation="MVRNASFRPTATTERHEALRRDVREFLHNELPADHEPGLGMVSG
                     HSPVFSRKLAARGLVGMAFPVAYGGHGRSVLDRFVVIEELLAAGAPVAAHWTADRQSG
                     PTILRFGTD"
     gene            complement(344..1096)
                     /locus_tag="VFX21_02170"
     CDS             complement(344..1096)
                     /locus_tag="VFX21_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5094785.1"
                     /translation="MTREASGGVTMRSAGGVATVTLSSQRVHNALTPAMARELLACCD
                     EIDADSQVGAAILIGAGTSFCSGADRRTWDVDGDQVAEPRYADTGVVYESFLRFGALA
                     VPTIAAVRGNAIGAGLNLMLAADMRLVSTTARIFPGFVRIGLHPGGGYFTLSNRVAGR
                     EATAAMGLFDEEIDGARAVALGLAWELCADEALEDRAQELAARVARDPQLARRVVCSF
                     REELGPPGIPWAVASEYERASQMWSQRRRDSA"
     gene            complement(1122..1823)
                     /locus_tag="VFX21_02175"
     CDS             complement(1122..1823)
                     /locus_tag="VFX21_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5094786.1"
                     /translation="MTSPVIVASGLKSGYGPIPVLHDIDIRVHAGELVALLGPNGAGK
                     TTTLLTIAGELPLMGGTVDLFGRRARGPLQRRAKSGLGLVTEERSVFARLSTRDNLRV
                     GRVDIEAGFRLFPELADRRDTLAGDLSGGEQQMLTLARALLRSPKALLADELSLGLAP
                     LVVDRLLRAVRRAADESGVGVLLVEQHVEEVLEFADRVYVVARGRVVFEGTVGQFRGR
                     RDEIEAAYLGTPSEA"
     gene            complement(1827..>3182)
                     /locus_tag="VFX21_02180"
     CDS             complement(1827..>3182)
                     /locus_tag="VFX21_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEX5094787.1"
                     /translation="IGNLRRGRAGRGFIAVRSNERAAAALGVNVVRSKLLAFALGSAV
                     AGLGGVLLAFKSHIVIFGSYDMFASISAVTFATIGGVGYLAGALLGAGLAAGGIGSWI
                     LNRYGGLDPWLGVIAGASVLLMLMQNPDGLAGAASHGHADPLTNRLVRSARRLRFGSV
                     TGEAEVRSAPPLADVWRAPADAVLSVDDLTVRFGGVTALAHLSLTVRPGEVVGLIGPN
                     GAGKTTAIDAITGFVRPTSGSVSLGTTNLDHSSVHRRAALGLTRSFQSVELFDEMTVL
                     ENLQVASDRRDLTAYLSNLVDTRRPELPPIVDAAVATFGLEPVLTTNPPQLSYGSRRL
                     VGLARAVATRPCVLLLDEPAAGLNRHESRELADAIRNLASQCGIGILLVEHDMSVVMA
                     VCDRITVLDFGSTIADGTPAEIRHDPAVIAAYLGTPRQDTDTSAVVSGAPLGAQLGED
                     R"
BASE COUNT          575 a         1116 c         1044 g          447 t
ORIGIN      
        1 catccgtccc gaaccgcaag atggtgggtc cactctggcg gtccgccgtc cagtgcgcag
       61 cgacgggcgc gcccgcggcg aggagctcct cgatgacgac gaaccggtcg agcacggaac
      121 gtccgtggcc gccgtacgcc accggaaagg ccatgccgac caatccgcgc gccgcgagct
      181 tcctgctgaa caccggcgag tggccggaca ccatcccgag cccgggctcg tggtcagcgg
      241 gcaactcgtt gtgcaggaac tcgcgtacgt cgcgccgcaa cgcctcatgg cgctcggtcg
      301 tggcggtcgg acggaaggat gcgttgcgaa ccacggccgg cacctacgcg gagtcgcgcc
      361 tgcgctgcga ccacatctga ctcgcgcgtt cgtactcgct ggccacggcc cacgggatgc
      421 ccggcgggcc cagctcctcg cgaaacgagc acacgacgcg tcgcgccagc tgcgggtcac
      481 gcgccacacg cgccgccagt tcttgcgcgc ggtcctccaa tgcctcgtcg gcacagagct
      541 cccacgccag accgagggcg accgcgcgag caccgtcgat ctcctcgtcg aagagtccca
      601 tcgcggcggt tgcttcacgt cccgcgacgc ggttcgaaag cgtgaagtag ccgccacccg
      661 gatggagccc gatacgcacg aatccgggaa agatgcgcgc cgtggtcgag accagacgca
      721 tgtccgcggc caacatcagg ttgagccccg cgccgatcgc gttgccgcgt accgccgcga
      781 tcgtcgggac ggcgagcgca ccgaagcgga ggaagctctc gtagacgacg ccggtgtccg
      841 cgtacctggg ctccgcgact tggtcgccgt cgacgtccca ggtgcggcgg tctgcgcccg
      901 agcaaaagct cgtgccggca ccgatcagga tcgcggcgcc gacctgcgaa tcggcgtcga
      961 tctcgtcaca gcaggcgagc agttcgcggg ccatggccgg ggtcagggcg ttgtggacgc
     1021 gttgtgacga gagcgtgacg gtcgcgacac cacccgcgct ccgcatcgtc actcccccgg
     1081 aggcctcgcg cgtcatcgca ctattgtata cgaaattgca ttcacgcttc gctcggcgtt
     1141 ccgaggtagg cggcctcgat ctcgtcgcgc cgaccgcgga actgcccgac ggtgccctcg
     1201 aagacgacgc gcccgcgcgc gacgacgtac acccggtcgg cgaactccaa gacctcttcg
     1261 acatgctgct cgacgaggag cacgccgacc ccggactcgt cggcggcgcg gcgcaccgcc
     1321 cgcaacagtc gatccacgac gagcggggcg aggcccagtg acaactcgtc ggcgaggagt
     1381 gctttgggcg agcgaagcaa cgcgcgagcc agtgtcagca tctgctgctc gcccccggag
     1441 agatcgccgg cgagggtgtc gcgtcgatcc gccagttcgg ggaacaggcg aaagcccgct
     1501 tcgatgtcga cgcgtccaac acgaaggttg tcgcgcgtcg acagtcgcgc gaacaccgac
     1561 cgttcctccg tgaccaggcc gagcccagac ttggcgcgcc gttgtagcgg tccgcgcgcg
     1621 cgtcgcccga acagatcgac ggtgccgccc atcagcggaa gttcaccggc aatcgtcagc
     1681 aacgtcgttg tcttgccggc accgtttggc ccgagcaacg cgaccagttc tcccgcgtgc
     1741 acgcgaatgt cgatgtcgtg cagcaccgga atcggcccgt agccggactt gagaccgctc
     1801 gccacgatga ccggtgacgt catcggctac cgatcctctc cgagctgcgc accgagcggc
     1861 gctcccgaca cgaccgcgct cgtgtcggtg tcctggcgcg gggtgcccag atacgcagcg
     1921 atgaccgcag gatcgtggcg gatctcggca ggcgtgccgt cggcaatcgt cgaaccgaag
     1981 tccaacacgg tgatccgatc gcagacggcc atgacgacac tcatgtcgtg ctcgacgagg
     2041 aggatcccga taccgcactg cgacgcaaga ttcctgatcg cgtcggccaa ctcgcgactc
     2101 tcgtgacggt tcagccccgc agccggttcg tccagcaaca agacacacgg gcgtgtcgcg
     2161 acggcgcgcg ccaagccgac gaggcgccgg ctcccgtacg acagctgcgg aggattcgtc
     2221 gtcagcaccg gctcgagacc aaacgtggct accgcggcat ccacgatcgg cggcagctcg
     2281 ggccggcgcg tgtcgacgag attggagaga tacgccgtga gatcgcgccg gtccgacgca
     2341 acctggagat tctcgagaac cgtcatttcg tcgaagagct cgaccgattg gaacgagcgc
     2401 gtcaaaccga gcgcggcgcg tcggtgcacg ctcgagtgat cgagattcgt cgtaccgagt
     2461 gacaccgatc cgctcgtcgg acgcacaaag cccgtgatgg cgtcgatggc cgtcgtcttg
     2521 ccggcgccgt tcggaccgat caatccgacc acttcgcccg gccgaacggt caacgataag
     2581 tgcgcgagcg cggtgacgcc tccgaagcgc accgtaaggt cgtcgacgga gagcacggcg
     2641 tccgctggtg cacgccacac gtccgcgagt ggcggggcgc tccgaacctc cgcttcgccc
     2701 gtgaccgaac cgaaccgcaa gcgacgggca ctgcgcacga gacgattggt cagcgggtcg
     2761 gcgtgtccgt gcgaagcagc acccgcgaga ccatcggggt tttgcatgag catgagcagc
     2821 acggacgcgc cggcgatcac cccgagccag gggtcgaggc caccgtaccg attgaggatc
     2881 cacgacccga taccgccggc ggccagaccc gccccgagca atgcgccggc gagatagccg
     2941 acaccaccga tcgtcgcgaa ggtcactgcg ctgatcgacg caaacatgtc gtagctgccg
     3001 aatatcacga tgtgcgactt gaacgccaag agcacgccgc cgagaccggc aacagcagaa
     3061 ccgagcgcga acgcaaggag tttgctgcgc acgacgttga caccgagcgc ggcggcagcc
     3121 cgctcgttcg atcgcactgc gatgaagccc ctgcccgcac gaccacgacg caggttcccg
     3181 at
//
LOCUS       DASUGA010000115         3334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_50798, whole genome shotgun sequence.
ACCESSION   DASUGA010000115 DASUGA010000000
VERSION     DASUGA010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50798"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..133
                     /locus_tag="VFX21_02185"
     CDS             <1..133
                     /locus_tag="VFX21_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094788.1"
                     /translation="WCRDRIAHYKCPRRVVFLEALPRHDNGKLYKNQLREQLRDEDR"
     gene            133..1029
                     /locus_tag="VFX21_02190"
     CDS             133..1029
                     /locus_tag="VFX21_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013805761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5094789.1"
                     /translation="MGICDGRVVIVTGAGRGIGREHALSFAREGAKVVVNDLGGDMHG
                     DGGSLSPAMEVVEEIKAMGGEAVADGENVADFAGAGRMVQKAIDTFGHLDTLVNNAGI
                     LRDRMLTNMEESEWDAVIAVHLKGAFGPTRHAASYWRDQSKAGTPVKARVVNTSSPSG
                     IFGNAGQTNYGAAKAGVAAFTIICGLELGRYGVCTNAIAPVARTRMTENLGMGAAPEG
                     TFDALDPANISPLVVWLGSNDNEDVSGRVFLVDGDRVSVAEGWHRGPTARNDGLRWEP
                     SQLTKVVPELVSQATEPSNMFS"
     gene            1040..1903
                     /locus_tag="VFX21_02195"
     CDS             1040..1903
                     /locus_tag="VFX21_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX5094790.1"
                     /translation="MPMNLDSVGAVSQPAKHSWTHKDSLIYALGIGAGQTDPTGFELE
                     FTTENSKNVTQRVFPTMPVVLGMGGGPGLPSWGDFNFAMLLHGEQGVTVHGAIPAEGE
                     VETVTTIVGIYDKGKAAVVRLSADSTYTDSGKPAFQTRFAAFIRGEGGFGESRGPEIP
                     DPPATPETKPDHEMTFATRADQALLYRLSGDRNPLHSDPEFAKMAGFDKPILHGLCTY
                     GVTGRALLHELCGSDVEKFGSMDVRFSKPVMPGDTLTVKMWDQGSGSYIFETVTQDGA
                     AVIKGGQFKTR"
     gene            complement(1904..2758)
                     /locus_tag="VFX21_02200"
     CDS             complement(1904..2758)
                     /locus_tag="VFX21_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX5094791.1"
                     /translation="MTRSVLAIDIGGTKLAAGVVGDDGTLIEHRRVPTPTGEDPEVLW
                     NTLTALIDGCTATDDAVAVGVGCGGPMSLGGEHVSPLNIPAWRNFPLRARLAEHCALP
                     VWVDNDAKALALGEGWVGAATGERDFIGMVVSTGIGGGIMCDGRLLDGAGGNAGHVGH
                     VIVEPDGHECGCGARGCVEAEASGSAIERITGRPAADAPLDVRVRCGTLVGRAIASVA
                     NLLDLQLAVVSGGVALGYGDVFFEAAQAEVDARCRLDFSRSTLVVPGALRERGPLVGA
                     ARVAFLRL"
     gene            complement(2755..>3334)
                     /locus_tag="VFX21_02205"
     CDS             complement(2755..>3334)
                     /locus_tag="VFX21_02205"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX5094792.1"
                     /translation="TPDDAIVVVVGDVAGHGITAASLMGRVRNALRAYANEDSDPASI
                     LLRLHRLLRSQDDEEMVTAFVARHDAKTNTISWSRAGHPPPLLVQPDGTARFLDDVNG
                     APLGTMVREYQTAHAQLDPGALLVCYTDGLVERRDRILDDGLAWLADRVSEYASDPIE
                     TLCNKLVDDPFVPHPAPDDVCIVALRVAPESA"
BASE COUNT          555 a         1123 c         1154 g          502 t
ORIGIN      
        1 gtggtgccgg gaccggatcg cgcactacaa gtgtccccgg cgcgtcgtgt tcctcgaggc
       61 cctgccccgg cacgacaacg gcaagctcta caagaaccag ctgcgcgagc agctccgaga
      121 tgaggatcgc taatgggtat ctgtgacggc cgcgtggtca tcgtgaccgg tgcgggacga
      181 gggatcggac gggagcacgc gctttccttc gcccgcgaag gcgccaaggt cgtggtgaac
      241 gacctcggcg gcgacatgca cggcgacggc ggctcgctga gcccggccat ggaagtggtc
      301 gaggagatca aggcgatggg cggcgaagcc gtcgccgacg gcgagaacgt cgccgacttc
      361 gccggcgcgg ggcgcatggt gcagaaggcg atcgacacgt tcggtcacct cgacacgctc
      421 gtgaacaacg ccggcattct gcgcgaccgc atgctcacca acatggaaga gagcgagtgg
      481 gacgcggtca tcgccgtgca cctcaagggc gcgttcggcc cgacccgcca cgcggcgtcg
      541 tactggcgtg accagtcgaa ggccggcacg ccggtcaagg cgcgcgtcgt caacacgtcg
      601 tcgccatcgg gcatcttcgg caacgcgggc caaacgaact acggcgcggc caaggcgggc
      661 gtcgccgcgt tcacgatcat ctgcggcctg gaacttgggc gctacggcgt gtgcaccaac
      721 gcgatcgctc cggtcgcgcg cacgcgcatg accgagaacc tcggcatggg tgcggcgccg
      781 gaaggcacgt tcgacgcgct cgacccggcg aacatttcgc cgctcgtcgt gtggctcggt
      841 tcgaacgaca acgaagacgt cagtggccgc gtcttcctcg tcgacggcga ccgcgtgtcg
      901 gtcgccgagg gctggcaccg cggcccgacc gcgcgcaacg acggtttgcg ctgggagccg
      961 tcgcagttga ccaaggtcgt ccccgagctc gtgtcgcagg cgaccgagcc ctcgaacatg
     1021 ttctcgtagg gagacacaca tgccgatgaa tctggattct gtgggcgcgg tttcgcagcc
     1081 cgccaagcac tcgtggaccc acaaggactc gctgatctac gcgctcggta tcggtgccgg
     1141 ccagaccgac ccgaccgggt tcgaactcga attcacgacc gagaattcga agaacgtgac
     1201 gcagcgcgtg ttccccacga tgccggtggt acttggaatg ggcggcggac cgggcctgcc
     1261 gagttggggt gacttcaact tcgcgatgtt gttgcacggc gagcagggcg tgaccgtgca
     1321 cggcgcgatt ccggccgaag gcgaagtcga gaccgtgacg acgatcgtcg gcatctacga
     1381 caagggcaag gccgcggtcg tccgcttgtc ggccgactcg acctacaccg acagcggcaa
     1441 gcctgcgttc cagacccggt tcgcggcgtt cattcgcggc gaaggcggct tcggcgagtc
     1501 gcgcggcccg gagattccgg acccgccggc cacgccggaa acgaagcccg accacgaaat
     1561 gacgttcgcg acgcgggccg accaggcgtt gctctaccgc ctctcgggcg accgcaaccc
     1621 gctgcactcc gaccccgagt tcgccaagat ggccggcttc gacaagccga tcttgcacgg
     1681 gctctgcacc tacggcgtga ccggccgggc gttgctgcac gagctgtgcg gctcggacgt
     1741 cgagaagttc ggctcgatgg acgtgcggtt ctcgaagccg gtcatgcccg gcgacacgct
     1801 caccgtgaag atgtgggacc aggggagtgg ctcgtacatc ttcgagaccg tgacccagga
     1861 cggcgccgcc gtcatcaagg gcggccagtt caaaactcgg tagctacaac cgtaggaatg
     1921 cgacgcgcgc ggctcccacg agggggccgc gttcgcgtag cgcgccgggt acgacgagcg
     1981 tgctgcgcga gaagtcgagg cggcaacgcg cgtcgacctc ggcttgggcg gcctcgaaga
     2041 acacgtcgcc gtagccgagc gcgacaccac cgctgaccac cgcaagttgg aggtcgagca
     2101 ggttcgcgac cgatgcgatc gcgcggccga cgagcgtgcc gcaccgcacg cgcacgtcga
     2161 gcggtgcgtc ggccgcgggc cggccggtga tgcgctcgat cgcactgccc gacgcttccg
     2221 cttcgacgca gccgcgcgcg ccgcaaccac attcgtgccc gtcgggttcg acgatgacat
     2281 gaccgacgtg accggcgttg ccgcccgctc cgtcgagcaa ccgcccgtcg cacatgatgc
     2341 cgccgccgat tccggtcgac acgaccatgc cgatgaagtc gcgttcgccg gtggcggcgc
     2401 cgacccagcc ttcaccgagt gcgagcgcct tggcgtcgtt gtcgacccac accgggagcg
     2461 cgcagtgctc cgcgagacgc gcgcgcaatg ggaagttgcg ccacgccgga atgttcagcg
     2521 gcgagacgtg ttcgccgccg aggctcatcg gccccccgca cccgacgccg accgccacgg
     2581 catcgtccgt cgcggtacaa ccgtcgatca gtgcggtcag cgtgttccac aacacctcgg
     2641 ggtcttcgcc ggtcggtgtc ggaacgcggc gatgttcgat cagcgtgccg tcgtcgccga
     2701 ccactccggc cgcaagcttg gtgccgccga tgtcgatcgc gaggaccgat cgcgtcacgc
     2761 agactccggc gcgacgcgca aggcgacgat gcacacgtcg tccggtgcgg ggtgcggcac
     2821 gaacggatca tccacgagct tgttgcacag cgtttcgatc ggatcactcg cgtattcgga
     2881 gacccggtcc gcgagccacg cgagcccgtc gtcgaggatg cggtcgcgcc gttcgacgag
     2941 gccgtcggtg tagcacacga gcagcgcacc gggatcgagc tgcgcgtgcg cggtctggta
     3001 ctcccgcacc attgttccga ggggcgcgcc gttgacgtcg tcgaggaagc gcgcagtgcc
     3061 atcgggctgg accaagagcg gcgggggatg gccggctcgc gaccacgaga tggtgttcgt
     3121 cttcgcgtcg tggcgcgcga cgaacgccgt caccatttcc tcgtcgtcct gcgaacgaag
     3181 caagcggtgc agtcgcagga ggatgcttgc cggatccgag tcttcgttcg cgtacgcgcg
     3241 tagtgcgttc cggacccggc ccatcaagct ggcggcggtg atgccgtgcc cggcgacgtc
     3301 gccgacgacc accacaatcg cgtcgtcggg cgtg
//
LOCUS       DASUGA010000116         2023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_468308, whole genome shotgun sequence.
ACCESSION   DASUGA010000116 DASUGA010000000
VERSION     DASUGA010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468308"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VFX21_02210"
     CDS             <1..312
                     /locus_tag="VFX21_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094793.1"
                     /translation="RFALYTPVVQLADGAPALDTGLALREDANAIDALCRLALRTYDD
                     WRTEDSDQPVRVFVDGNERALNADGTFAADGDVILVRQGSACTRVDGRTTLPFTDRRL
                     A"
     gene            309..1046
                     /locus_tag="VFX21_02215"
     CDS             309..1046
                     /locus_tag="VFX21_02215"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5094794.1"
                     /translation="MTRISVVMPAYNEAEILATSVKDVVEGLRERNESFEVLIVENGS
                     TDGTLALAEQLASEYAEVRAEHRDEANYGRALRHGLLEAQGDAVVNFDTDYFDLDFLA
                     AAVDRVLTTGGPAIVVGSKRAAGANDTRAPLRKMATWIFSTILRVAFGLKVSDTHGMK
                     AMRRAAVAPYAEACFSGQDLFDTELILRIERAGLGTGEIPVAVHELRPARSSFLSRVP
                     RTLRGLCNLRIALWKEARAQKSSPAQA"
     gene            1001..>2023
                     /locus_tag="VFX21_02220"
     CDS             1001..>2023
                     /locus_tag="VFX21_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5094795.1"
                     /translation="MEGSSRSEEQSGPSLTLAGGRILNGEGGQSASPAWITIADGRIV
                     ATGAGAAPPDAVDLGNALLAPGFLDLQVNGWDDVDFARSSVADIVDAVDRMAAGGTTG
                     VLLTICSAPLDRYDAMLQRAQDATKERSDVVMGVHLEGPFLGGAPGAHPVEVLRPTDL
                     RWIEALWDTAGSIIKLVTLAPEADPELQATRFLSERGVVVALGHSTVDYDGARSAADA
                     GASLVTHLFNGMGPMHHRAPGLPGAALDDPRLVPSLIADFVHVHPALVKLALTARPDA
                     VLVTDAVRASDTVLPDGTLAGSTLTMEAAVQNVAGLGFAPPRALRYATANAARVLGLA
                     DRGRIAP"
BASE COUNT          297 a          735 c          669 g          322 t
ORIGIN      
        1 cggttcgccc tctacacacc cgttgtgcag ctcgccgacg gcgcgcccgc gctcgacacc
       61 ggcctcgccc tgcgcgagga cgccaacgcg atcgacgcac tctgccgcct cgcgctccgg
      121 acgtacgacg actggcgcac cgaagattcg gaccagccag tgcgggtgtt cgtcgacggt
      181 aacgaacgcg ccctgaacgc cgacggcacg ttcgccgccg acggcgacgt catcctcgtc
      241 cgtcaggggt ccgcctgcac ccgcgtcgac ggccgcacca cgttgccgtt caccgatcgg
      301 cgactcgcat gacccgcatc tccgttgtca tgcccgcgta caacgaggcc gaaattctcg
      361 ccacgtcggt gaaggatgtg gtcgaaggct tgcgcgagcg caacgaatcg ttcgaggtgt
      421 tgatcgtcga aaacggctcg accgacggca ccctcgcgct cgccgaacaa ctcgcttccg
      481 aatacgccga agtgcgcgcc gagcatcgcg acgaagccaa ctacggccgc gcgttgcgac
      541 acggcctgct cgaggcgcag ggcgatgcgg tcgtcaactt cgacaccgac tacttcgatc
      601 tcgacttcct cgccgccgcc gtcgaccgag tcctgacgac cggcggaccg gcgatcgtgg
      661 tgggctccaa gcgcgccgcc ggcgcgaacg acacccgggc gccgctgcgc aagatggcca
      721 cctggatttt cagcacgatt ctgcgggtcg cgttcggttt gaaggtctcg gacacgcacg
      781 gcatgaaggc catgcgccgg gccgcagtcg cgccgtacgc cgaggcgtgc ttctcgggcc
      841 aggatctctt cgacaccgaa ttgatccttc ggatcgaacg ggccgggctc gggaccggcg
      901 agattccggt tgccgtgcac gaactgcgtc ccgctcgcag ctcgttcctg tcccgcgtcc
      961 cgcgtacgct gcgcggcctg tgcaacctgc ggatcgcgct gtggaaggaa gctcgcgctc
     1021 agaagagcag tccggcccaa gcctgacgct cgccggcggt cgcatcctca acggtgaagg
     1081 cggccagtcc gcgtcgccgg cgtggatcac gattgccgac ggccgcatcg tcgcgaccgg
     1141 cgcgggcgcg gcgccgcccg acgccgtcga tctcggcaac gcgttgctcg ccccgggctt
     1201 cctcgatctt caggtcaacg gctgggacga cgtcgacttc gcccgatcgt cggtcgccga
     1261 catcgtcgac gcggtcgatc gcatggccgc gggcggcacg accggcgtgc tgctgaccat
     1321 ctgcagcgct ccgctcgacc gctacgacgc gatgctgcaa cgggcgcaag acgcgacgaa
     1381 ggaacgttcc gacgtcgtga tgggcgtgca cctggaaggg ccgtttctcg gtggtgcgcc
     1441 cggcgcgcat ccggtggagg tgttgcgtcc cacagatctc cggtggatcg aagcgttgtg
     1501 ggacacggcg ggttcgatca tcaagctcgt cacactcgca cccgaagcgg atcccgaact
     1561 gcaggcgact cgctttctgt ccgagcgtgg cgtcgtcgtc gcgctgggac attcgaccgt
     1621 cgactacgac ggcgcgcgtt ccgcggccga cgccggcgcg tcgctggtga cgcacctttt
     1681 caacggcatg ggtccgatgc accaccgcgc gccggggttg cccggggccg cgctcgacga
     1741 ccctcgcctc gtaccgtcgc tcattgccga cttcgtgcac gtgcatcccg cgctcgtgaa
     1801 gcttgcgctg acggcccggc ccgacgcggt gctcgtcacc gacgcggtac gcgcgtccga
     1861 caccgtgctc cccgacggca cgctcgccgg ttcgaccttg acgatggaag ccgcggtgca
     1921 gaacgttgcc ggactcgggt tcgcgccgcc gcgcgcgctg cggtacgcga ccgcgaacgc
     1981 ggcgcgcgtg ctcggtctgg ccgaccgcgg ccgcatcgcg ccc
//
LOCUS       DASUGA010000117         5918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_623254, whole genome shotgun sequence.
ACCESSION   DASUGA010000117 DASUGA010000000
VERSION     DASUGA010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623254"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VFX21_02225"
     CDS             complement(<1..216)
                     /locus_tag="VFX21_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5094796.1"
                     /translation="MEEFLALAEESPRRAFAVGDVLIADGASVPALYVLLDGALRIEK
                     SGAPIANVTDPGACVGEMSLLLGIPATA"
     gene            304..720
                     /locus_tag="VFX21_02230"
     CDS             304..720
                     /locus_tag="VFX21_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094797.1"
                     /translation="MRRYLIVANRTLTGPHLVAEIVKLAAEGDAEFHLLVPMHPAHAT
                     FWTEGQAQAGARANLEQAIHTLRDAGIDATGEVGEENVVRAVGDVIIGQPVDEIILST
                     LPPGVSRWMKRDLPHKLARTYHLPVTHIVAERVPAH"
     gene            complement(721..1698)
                     /locus_tag="VFX21_02235"
     CDS             complement(721..1698)
                     /locus_tag="VFX21_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013475321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX5094798.1"
                     /translation="MAARNVGETRDGVELTNLDQPLFDGAGATKRDLIDYLDGVRSRI
                     LPVLEDRPLSVIRLMRGQEAFMQKNVPKYTPDFVRTVKFWAETSKRDVNYALCNDRKT
                     LLWFANQRAIEYHPALVRVQRPDHVTHMVLDLDPPGDGDNSSAAQFKMAVQVAHLVRR
                     ALADVGLTGAVKTSGAKGVHIFVPVDDETSIEDVAHAARAVAVRTERLDPKLATTAFT
                     KEDRGHKVFVDSTRVGGATVICACSPRVRPGVPVSFPLSWDEVDDVTPTDFTVHTALD
                     RLGDTDPWREHMPKPQALPADLVAEGNEIPVARVQAMHEGKRRKREREK"
     gene            complement(1822..2229)
                     /locus_tag="VFX21_02240"
     CDS             complement(1822..2229)
                     /locus_tag="VFX21_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008322700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX5094799.1"
                     /translation="MSALAVGQTARRTRTVQARDIELFTEMTGDRNPLHYDESLAAAS
                     RFGGIIVQGGVTSGLLNALVAEDLPGPGSVFLHVDWSFRAPVAPGDTLLAEAEVLSVR
                     DDKPICELRTTITNQAGTVVLDGTALVYREPLG"
     gene            complement(2229..3401)
                     /locus_tag="VFX21_02245"
     CDS             complement(2229..3401)
                     /locus_tag="VFX21_02245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5094800.1"
                     /translation="MNALIGSHESYDAIVVGARPAGAATAMLLGRAGRRVLLVDRSEY
                     GADTLSTHALLRSGVLQLHRWGLLDAVQRAVTPPVTATRFHYGDDVIDIAIRAENGVD
                     ALYAPRRTVLDPILVDAARDAGVEVVYKVRVTDVLRDPDDRVRGIRAQTLHGDDAIAT
                     APIVIGADGIHSSVARLVASETVHETTTASAFIYGYFDGFVPGAYDWFFRPGVSAGVI
                     PTNDGLANIFVGLPPARFSAEARGGEVDAAFRAVLREAAPEVAAQIAGRAPVARHRSF
                     PGQVGHLRRAHGPGWALVGDAGSYKDPLTAHGITAALRDAELLVRSLVSTGDAVQFET
                     MRDAFATPLLEVTASLAAYDWDIPEVMNLHQQLKRIITREVDMLTSFDALSASEAA"
     gene            complement(3433..3996)
                     /locus_tag="VFX21_02250"
     CDS             complement(3433..3996)
                     /locus_tag="VFX21_02250"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008884390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX5094801.1"
                     /translation="MTATTDRPARNGVDVPNLFATINAVRDDPGLAKFRFRATNRWVV
                     GTHSRTTIESFSGAGRNHMHAKDFAYDADHPAVLVGADQGPTPVEFLLHAIGACLMAG
                     VANIAAARGVTLTCVEAQIEGDVDIQGLLGLSDEVRNGYEQIRVTFHIEGDAPDEKLR
                     EIVEQSRNRSAVYDVLTNGVNVAIDVA"
     gene            4136..>5918
                     /locus_tag="VFX21_02255"
     CDS             4136..>5918
                     /locus_tag="VFX21_02255"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5094802.1"
                     /translation="MIFEFDEFELDTERFELRRRGETCHVEPQVFDVLRYLVEHRDRV
                     VPKEELLDNVWGDRFVSESALTSRIKVARQALDDTGRAQRMIATAHRRGYRFVATTTE
                     RVAAVDAAPTARPTSARRDIRYARSGDVNVAYEITGAGDVDIVLVPGFVSHLEIDWED
                     PRSADFLDRLGRIGRLIRFDKRGTGLSDRPAGLPDLETRMDDVRAVMDAAGRERAVLF
                     GYSEGGPMAALFAATYPARTRALVLFGTYARRTRTDDYPWAPTLEQRIAHAASTESEW
                     GIASGLLRMCPNADQAMAEWWQRRARAAASPGAVRALIEMNTMIDVRDVLPLVHVPTL
                     VLHRVGDTNMTIENGRYVAEHIPGARLIELHDTDDHVPWIGAATLLEPVEAFIAALDD
                     EQPMGATSDRALATTLFTDIVGSTDMNAEMGDARWSALLDRHDHIAREAIETWDGRWV
                     KSTGDGVLAVFETPVRALRAAIVVRTKLAELGIRIRAGLHASEIETRGNDIVGLGVTI
                     AARLVELGEPGEILVTSTVREIVGGSDVELVARGEHQLKGIPGRSVLYAVAMPEPVMP
                     SFARAPAATPERPARNVPKPADQLFGRD"
BASE COUNT          933 a         2037 c         2027 g          921 t
ORIGIN      
        1 ggccgttgcg ggaatgccga gcagcagcga catctcgccg acgcacgcgc ccggatcggt
       61 gacgttggcg atcggtgctc ccgacttctc gatgcgaagc gcgccgtcga gcaacacgta
      121 caacgccggc accgacgcac cgtcggcgat cagcacgtca ccgactgcaa acgcgcggcg
      181 cggactttcc tccgcgagtg cgaggaactc ttccatgacc gcagtgtcgc atacgcgtcg
      241 ttgctgacca gttgctgacg gcttatcgtt ggtggcaacg aacaggcaga ggaggcgtga
      301 ccaatgcgcc gatacctcat cgttgcgaac cggacgctca ccggtcccca cctggtcgcg
      361 gagatcgtga agcttgcggc cgagggagat gccgagttcc atctccttgt gcccatgcat
      421 ccggcgcatg cgaccttctg gaccgaaggt caggcgcagg ccggtgcccg cgccaacctc
      481 gagcaggcga tccatacgtt gcgcgacgcc ggcatcgacg cgaccggcga agtcggcgag
      541 gagaatgtgg tgcgtgcagt cggcgacgtg atcatcggtc agccggtgga cgagatcatc
      601 ctctcgacgt tgccgccggg cgtgtcccgc tggatgaagc gcgatctgcc ccacaagctg
      661 gcccgcacat atcacctgcc ggtcacgcac atcgtggccg aacgggtgcc cgcgcactaa
      721 ctacttctcg cgctcgcgtt tccggcgctt gccttcgtgc atcgcctgca cgcgggcgac
      781 ggggatctcg ttgccttcgg cgacgaggtc cgcgggaagt gcctggggtt tcggcatgtg
      841 ctctcgccac gggtcggtgt caccgagccg gtcgagcgcg gtgtgcacgg tgaagtcggt
      901 gggggtgacg tcgtcgacct cgtcccacga gagcgggaac gacaccggca cacccggccg
      961 cacccgcggg ctgcaggcgc agatgacggt cgcaccaccg acccgtgtcg agtcgacgaa
     1021 caccttgtgg ccgcggtctt ccttcgtgaa cgcggtcgtc gccagcttcg gatccagtcg
     1081 ctcggtgcgc accgcgaccg cgcgggcggc gtgcgcgacg tcctcgatcg acgtctcgtc
     1141 atcgaccggc acgaagatgt gcacaccctt cgcgccgctc gtcttcaccg cgccggtgag
     1201 cccgacgtcg gccagcgctc gccgcacgag gtgcgcgacc tgcaccgcca tcttgaattg
     1261 ggccgccgac gagttgtcgc cgtcacccgg ggggtcgagg tcgagcacca tgtgcgtgac
     1321 gtgatcgggt cgctgcaccc gcaccaacgc cgggtggtac tcgatcgccc gctggttcgc
     1381 gaaccacagc aacgtcttgc ggtcgttgca aagcgcatag ttgacgtcgc gtttcgacgt
     1441 ctcggcccag aacttcaccg tccgcacgaa gtcgggcgtg tacttcggga cgttcttctg
     1501 catgaacgcc tcttgcccgc gcatcaaccg gatcaccgac aacggccggt cctcgagcac
     1561 cggcaggatg cgactgcgca ccccgtccaa gtagtcgatg agatcgcgct tggtcgcgcc
     1621 cgcgccgtcg aacaacggct ggtcgaggtt cgtcagctcg acgccatccc gcgtctcgcc
     1681 gacattccgc gcggccatcg cgaccaacgc tagttggctc gaagttgtga cgtggaccgc
     1741 catatatggc catctagctc acaagaacga agacgcgacg cgcgccgcca gctgcaactc
     1801 ctcgatgctg atcgtcacgg ctcaacccaa cggttcgcgg tacaccagcg cagtgccgtc
     1861 cagcacgacc gtgccggcct ggttcgtgat cgtcgtgcgc agctcacaga tgggtttgtc
     1921 gtcgcgcacc gacagcacct cggcttcggc gaggagcgtg tcgcccggcg cgaccggtgc
     1981 ccggaagctc caatcgacgt gcaggaacac gctgcccgga ccgggcagat cctcggccac
     2041 caacgcgttg agcaaccccg acgtcacccc gccctgcacg atgatcccgc cgaaccgact
     2101 cgcagccgcg agcgactcgt cgtagtgcag cggattgcgg tcgcccgtca tctccgtgaa
     2161 cagttcgatg tcacgagcct gcacggtgcg agttcggcgc gccgtctgac cgacagcgag
     2221 cgcgctcatc acgcggcctc gctcgcgctc agggcgtcga acgacgtcaa catgtcgacc
     2281 tcccgcgtga tgatcctctt caactgctgg tgcaagttca tcacctcggg aatgtcccag
     2341 tcgtaggcgg cgagcgacgc ggtgacttcg agcaacggcg tcgcgaacgc gtcgcgcatc
     2401 gtctcgaact gcaccgcatc accggtcgac accaacgagc gcactagcaa ctcggcgtcg
     2461 cgcagggcgg ccgtgatgcc gtgggctgtc aagggatcct tgtacgaccc cgcgtccccg
     2521 accagcgccc acccgggacc gtgggcccgc cgcaggtgtc cgacttggcc ggggaacgac
     2581 cggtgccggg caacgggcgc gcgacccgcg atctgggccg cgacctcggg cgcggcctca
     2641 cgcaacaccg cgcggaacgc ggcgtccact tcacccccgc gcgcctcggc cgagaagcgc
     2701 gccgggggca ggccgacgaa gatgttcgcg aggccgtcgt tggtcgggat cacaccggca
     2761 ctgacgcccg gccggaagaa ccagtcgtac gcacccggta cgaacccgtc gaagtatccg
     2821 tagatgaacg cgcttgccgt cgtcgtctcg tgcacggtct cgctcgcgac gagccgagcg
     2881 acgctggagt ggatcccgtc ggcaccgatc acgatcggtg cggtcgcgat cgcgtcgtcg
     2941 ccgtgcaacg tctgcgcgcg gatgccgcgg acgcgatcgt cggggtcacg aagcacgtcg
     3001 gtgacgcgca ccttgtacac gacctccaca cccgcgtccc gcgccgcgtc gaccaggatc
     3061 gggtcgagca cggtgcgacg cggtgcgtac agcgcgtcga caccgttctc ggcgcggatt
     3121 gcgatgtcga tcacgtcgtc gccgtagtgg aagcgggtcg ctgtgaccgg tggggtcacc
     3181 gcacgctgta ctgcgtcgag gagaccccac cggtgcagtt gcagcacccc gctgcgcagc
     3241 aacgcatgcg tcgagagcgt gtcggcgccg tactcggacc ggtcgacgag cagcactcgc
     3301 cggccggccc ggcccagcaa catcgccgtc gcggcgcccg cgggccgggc acccacgacg
     3361 atggcgtcgt aactctcgtg actcccgatg agggcgttca tgatcggacc aatcactcga
     3421 agtcggactc ggttatgcga cgtcgatcgc gacgttcacg ccgttggtga gcacgtcgta
     3481 gaccgcggac cggttgcggg actgctcgac gatctcgcgc agcttctcgt ccggtgcgtc
     3541 gccttcgatg tggaacgtga cgcggatctg ctcgtagccg ttgcggactt cgtccgagag
     3601 gccgagcagt ccttggatgt cgacgtcgcc ctcgatctgc gcctcgacgc acgtgagcgt
     3661 cacgccgcgc gcggccgcga tgttcgcgac gccggccatg aggcaggcgc cgatcgcgtg
     3721 gagcaggaac tcgacgggcg tcgggccctg gtccgcgccg acgagcacgg ccgggtggtc
     3781 ggcgtcgtag gcgaagtcct tggcgtgcat gtggttgcgg cccgcacccg agaacgactc
     3841 gatcgtggtg cggctgtgcg tgccgacaac ccatcggttc gtggcgcgga agcggaactt
     3901 ggccaggccg gggtcgtcgc ggacggcgtt gatcgtggcg aagaggttgg ggacgtcgac
     3961 cccgttgcgg gccgggcggt cggtggtggc ggtcatcact ggctcctttg tcgaacactc
     4021 gttgttcgtg cgtcgatcat gcggatcgag gcccgggcgg gcttggagag gagctggcgg
     4081 agttgtggag aacgcgtgga ggcggctccc tccgggcgta ggccaagatg gggccgtgat
     4141 cttcgagttc gacgagttcg agctcgacac cgagcgcttc gagctgcgcc ggcgcggcga
     4201 gacgtgtcat gtcgagccgc aggtgttcga cgtgctgcgg tacctcgtcg agcaccgcga
     4261 ccgcgtcgtg cccaaagagg aattgttgga caacgtctgg ggcgaccgct tcgtgagcga
     4321 atcggccctg accagccgga tcaaggtggc ccggcaggcc ctcgacgaca ccggacgcgc
     4381 gcagcgcatg atcgcgactg cccaccggcg cggctaccgg ttcgtcgcca cgaccaccga
     4441 gcgagtcgcg gccgtcgacg cggcgccgac ggcgcgtccc acttcggccc ggcgcgacat
     4501 ccgttacgcg cgcagcggcg acgtgaatgt cgcgtacgag atcaccggcg cgggtgacgt
     4561 cgacatcgtg ctcgttcccg gcttcgtctc ccacctcgag atcgactggg aggacccgcg
     4621 ctcggccgac ttcctcgatc ggctgggccg catcggccgc ctgatccgct tcgacaagcg
     4681 cggcaccggt ctctcggatc gccccgccgg cctccccgac ctcgagaccc gcatggacga
     4741 cgtgcgcgcc gtcatggacg cggccggccg cgaacgggcg gtgctgttcg gatactcgga
     4801 gggcggcccc atggccgcgc tcttcgcggc gacgtacccg gcgcgcacac gcgcgctcgt
     4861 gctgttcggc acgtacgcgc ggcggacgcg caccgacgac tacccgtggg cgccgacgct
     4921 cgaacaacgc atcgcgcacg cggcatccac cgagtcggag tggggcatcg cgtccgggct
     4981 cctgcgcatg tgtccgaacg cggaccaggc gatggcggag tggtggcaac gccgggcccg
     5041 cgccgccgcg agcccgggcg cagtacgggc gctgatcgag atgaacacga tgatcgacgt
     5101 gcgcgacgtg ctgccgctgg tgcacgtgcc cacactcgtg ttgcaccgag ttggcgacac
     5161 gaacatgacg atcgagaacg ggcggtacgt cgccgagcac attcccggcg cccgcctcat
     5221 cgagctgcac gacacggacg accatgtgcc gtggatcggc gccgcgacgc tgctcgaacc
     5281 cgtcgaggca ttcatcgcgg cgctcgacga cgaacagccc atgggcgcga cgagtgaccg
     5341 cgcgctggcg acgacactgt tcaccgacat cgtcgggtcg accgacatga acgccgagat
     5401 gggagacgcg cgctggagcg cgttgctcga ccgccacgac cacatcgcgc gcgaggcgat
     5461 cgagacgtgg gacgggcgct gggtcaagtc gaccggcgac ggcgtgcttg cggtgttcga
     5521 gacgccggtg cgggcgctgc gcgctgcgat cgtggtgcgc acgaagctgg cagagctggg
     5581 catccgcatt cgggcgggac tgcatgcgag cgagatcgag acccgcggca acgacatcgt
     5641 cggtctcggc gtgacgatcg cggcgcggct cgtcgagctc ggtgaaccgg gcgagatcct
     5701 cgtgacgtcg accgtgcgcg agatcgtcgg cggctccgac gtcgagctgg tcgcgcgcgg
     5761 cgaacatcag ctcaaaggca tcccgggcag gagcgtcttg tacgcggtcg cgatgcccga
     5821 gccggtgatg ccgtcgttcg cgcgcgcgcc ggccgccacg cccgaacggc ccgcgcgcaa
     5881 cgtccccaaa ccggccgacc agctcttcgg acgcgacg
//
LOCUS       DASUGA010000118         2179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1996007, whole genome shotgun sequence.
ACCESSION   DASUGA010000118 DASUGA010000000
VERSION     DASUGA010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1996007"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VFX21_02260"
     CDS             <1..268
                     /locus_tag="VFX21_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEX5094803.1"
                     /translation="LRLAARRERRASWSASTMQRLECIGKKAGRPRAPAETPTEYAQA
                     LADQLHEPQLVTVGAALDTDSYSAAGAPPEARADADAVLSSLRP"
     gene            305..901
                     /gene="pyrR"
                     /locus_tag="VFX21_02265"
     CDS             305..901
                     /gene="pyrR"
                     /locus_tag="VFX21_02265"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX5094804.1"
                     /translation="MPARSHEHAREPSREDESGAVLRTRVFDAADLRRALTRIAHEIV
                     ERNHGADEVVLVGMYTRGVALSRRIAAAIGSFEAVEPALGALDVAFYRDDIGLRPVAP
                     LGPTEVPDITGKVVVLVDDVLFTGRTARAALDALLELGRPRAVQLAVLVDRGHRELPL
                     RADYVGKNLPTRIREDVRVRLEEVDGGEDGVELWGDPE"
     gene            898..1830
                     /locus_tag="VFX21_02270"
     CDS             898..1830
                     /locus_tag="VFX21_02270"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX5094805.1"
                     /translation="MTLKHLLSVDDLGGRPGIEQMLDLTDSFVELQQREIPKVPALRA
                     KTVVSLFYEDSTRTRLSFETAAKRLGADTMTFSVSTSSVKKGESLLDTVQTIEAMGID
                     AIVCRHGAAGAPNRVAQWSTANVVNAGDGRHEHPTQALLDAFTLRRHRGPSLDGCRVA
                     IVGDIRNSRVARSDVKAFDALGCELTLVGPPTLMPERLDGWPVRVAYDLDDVLADVDV
                     VYLLRIQRERIGQALFPTIREYATRWGLTAERAARLKPDTFVMHPGPMNRGIEIAGEV
                     ADSARSLVTEQVANGVAVRMAVLWTLLGSGGPIV"
     gene            1853..>2179
                     /locus_tag="VFX21_02275"
     CDS             1853..>2179
                     /locus_tag="VFX21_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018761423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5094806.1"
                     /translation="MLDESGERAADVRVVDGAIAEVGSGLAASAGATVLDAEGCVVTP
                     GLVDIQVHFREPGREDSETIETGARAAALGGCTAVVCMPNTEPPLDDAAVVQTVLERA
                     RAVGLCD"
BASE COUNT          340 a          726 c          774 g          339 t
ORIGIN      
        1 cctgcgcctg gcggcccggc gggaacgccg cgcgtcatgg agcgcaagca cgatgcaacg
       61 gctcgagtgc atcgggaaga aggcgggccg gccgcgggcg ccggccgaga cgccgacgga
      121 gtatgcgcag gccctggccg atcagctcca cgagccccaa ctcgtgacgg tgggcgcggc
      181 cctggacacc gactcgtatt cggcggccgg tgcgccgcca gaagcgcgag cagacgcaga
      241 cgcggtgcta tcctcgctcc gaccgtgaag gagatcccgt gaggtctcta cggaaggaag
      301 acgcatgcca gctcgttctc acgagcatgc gcgcgagcct tcgcgcgaag acgaatccgg
      361 cgcagtcctg cgcactcgtg tcttcgatgc cgctgatctc cggcgggccc tgacacgcat
      421 cgcgcacgag atcgtcgagc gcaatcacgg cgccgacgag gtggtgctcg tcgggatgta
      481 cacccgcggc gtcgcgctgt cccgccggat cgcggcggcg atcggctcgt tcgaggcagt
      541 cgagcccgcg ctcggtgcgc tcgacgtcgc cttctatcgc gatgacatcg ggctgcgtcc
      601 ggtcgcgccg ctcggaccga ccgaggtccc cgacatcacc ggcaaggtcg tcgtcctggt
      661 cgacgatgtg ctgtttacgg gccgcaccgc ccgggctgcg ctcgacgcgc tgctcgaact
      721 cggacgcccg cgcgcggtgc agctggccgt tctcgtcgac cggggtcatc gcgaactgcc
      781 actgcgcgcc gactacgtcg gcaagaacct gcccacacgc atccgggaag acgtacgcgt
      841 gcgcctggaa gaagtcgacg gcggcgagga cggcgtcgaa ctctgggggg atccggaatg
      901 acgttgaagc atctgttatc agtcgacgac ctcggggggc gcccgggcat cgagcagatg
      961 ctcgacctca ccgactcgtt cgtcgaactc cagcagcgcg agatcccgaa agttcccgca
     1021 ttgcgcgcca agaccgtggt gtcgctgttc tacgaggact cgacccgcac caggttgtcg
     1081 ttcgagaccg cggcgaagcg gctcggcgcc gacaccatga cgttctcggt gtcgacgtcg
     1141 tcggtgaaga agggcgaaag cctgctcgac accgtccaga cgatcgaagc catgggcatc
     1201 gacgcgatcg tgtgccgcca cggcgctgcg ggcgcgccga atcgcgtggc ccagtggagc
     1261 accgcgaatg tcgtgaacgc gggcgacggt cgtcacgaac acccgaccca ggcgctgctc
     1321 gatgctttca cgctccgccg ccaccgcggg ccgtcactcg acggctgtcg cgttgcgatc
     1381 gtgggcgaca tccggaactc gcgcgtcgcg cgcagcgacg taaaggcgtt cgacgccctc
     1441 ggctgtgagc tcacgctcgt tggtccgccc acgttgatgc cggaacggct cgacggttgg
     1501 ccggtgcgcg tcgcctacga cctcgacgac gtcctcgccg acgtcgacgt cgtgtacttg
     1561 ctgcggatcc agcgggaacg catcgggcaa gcgctgttcc cgacgattcg cgaatacgcc
     1621 acgcgctggg gtctcactgc ggaacgggcg gcgcggctga agcccgacac gttcgtgatg
     1681 cacccggggc cgatgaatcg cgggatcgaa atcgccggtg aggttgccga ctccgcgcgc
     1741 tcgttggtga cggaacaagt tgccaacggt gtcgcagtgc gcatggcggt gttgtggacg
     1801 ctgctcggct ccggaggtcc cattgtctga gatttttgtc cttggtgggc gcgtgctcga
     1861 cgagagcggc gagcgcgcgg ccgatgtgcg ggtcgtcgac ggcgcgatcg ccgaagtcgg
     1921 ttccggcctg gcggcgagcg cgggagcaac cgtgctcgac gccgaagggt gcgtggtgac
     1981 gccgggactc gtcgacatcc aggtgcactt tcgcgaaccc ggtcgcgagg acagcgagac
     2041 gatcgagacc ggcgcccgcg ccgccgcgct cggcgggtgc actgcggtgg tgtgcatgcc
     2101 gaataccgag ccgcccctcg acgatgcggc cgtcgtccaa acggtgctgg aacgggcgcg
     2161 ggcggtcggg ttgtgcgac
//
LOCUS       DASUGA010000119         2339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1161944, whole genome shotgun sequence.
ACCESSION   DASUGA010000119 DASUGA010000000
VERSION     DASUGA010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161944"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1381)
                     /locus_tag="VFX21_02280"
     CDS             complement(<1..1381)
                     /locus_tag="VFX21_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007023694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX5094807.1"
                     /translation="MDPTASHKSFREREGHGAVDTDRAPISGGRAHHAVAVHEVVLLL
                     GTDVAAGLTTAEAEARLRRFGPNVLAETDAAGAVRRFLRQFHHPLIYVLLAAGVITLL
                     LGDRVDSAVIFGVVLVNAFVGFVQESKAEAARDVLRSMVRTDARVRRDGRLQTVRSEE
                     LVPGDVVALEAGDKVPADLRLVELAELEADESALTGESLPVAKDEVVVAAATPVADRH
                     NMVYSGTLVTRGTGLGVVVATGSETELGEIHRLVGTAETMATPLTRTLARFSKVLTAA
                     ILGLACFTFAVGVARGEDVSEMFNAAIALAVGAIPEGLPAAVTITLAIGVHRMAQRRA
                     VIRRMPAVETLGSTTVICSDKTGTLTENQMTVRTIWTPSDAFDVSGSGYRPQGAIRAR
                     SGEPVAPDLHEALRWTLLVGAACNDARLTDRNGAYEVIGDPTEGAMLVVGAKAGMDAT
                     WVRQHFPRVA"
     gene            complement(1393..>2339)
                     /locus_tag="VFX21_02285"
     CDS             complement(1393..>2339)
                     /locus_tag="VFX21_02285"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5094808.1"
                     /translation="RPHDGAGDVEVAPGVRLRFSPAGHILGAASVWLSDGNRSVLFSG
                     DLGRSDDWVMRPPAAPLAADHVVVESTYGNRQHPDEDPMDVLAEVVTSTARRGGIVLV
                     PVFAVGRAQTVLHLLARLRDAGRIPEMPTYLNSPMAIDATELFIRSEGQHRLTPAEVV
                     EMGKNVELVRTVEESIALTSRPGPMIVLSASGMLAGGRVLHHLFQVAPDHRSTIVLAG
                     YQAAGTRGEALANGARTLRVFGENVPVRARVVQLESLSAHADGDELMAWLRAAPAAPD
                     GVSVVHGEASAADTLRRRIHLELDWPARVPAYGESAAV"
BASE COUNT          370 a          870 c          758 g          341 t
ORIGIN      
        1 tcgcgacccg cgggaaatgt tgtcgcaccc atgtcgcgtc catgcccgcc tttgcgccga
       61 cgaccaacat ggcaccctcg gtgggatccc cgatgacctc gtacgccccg ttcctgtcgg
      121 tgagccgcgc gtcgttgcac gcggctccga cgagcagggt ccagcgcaac gcctcatgga
      181 ggtccggggc aaccggttcg ccggagcgcg cacggatcgc accctgcggc cggtatccgg
      241 atccgctgac gtcgaacgcg tcgctcggcg tccagatcgt ccgcaccgtc atttggttct
      301 cggtcaatgt cccggtcttg tcggaacaga tcaccgtcgt gctcccgagc gtctcgaccg
      361 ccggcatgcg acgtatcacc gcgcgccgct gcgccattcg gtgcacgccg atggccaacg
      421 tgatggtcac cgcggcgggc aggccctcgg gaatggcgcc caccgccaac gcgattgccg
      481 cgttgaacat ctcgctcacg tcctcgccac gcgcaacgcc gaccgcgaac gtgaagcacg
      541 cgagcccgag gatcgcggcc gtcaggacct tcgagaaccg cgccaacgtg cgcgtcagtg
      601 gcgtcgccat cgtctccgcg gtgcccacca ggcggtggat ctcgccgagc tccgtctccg
      661 atcccgtggc gacgacgacg ccgaggcccg tgccgcgggt gacgagcgtg cccgagtaga
      721 ccatgttgtg ccggtccgcg accggtgtcg ccgccgcgac gacaacctcg tccttggcaa
      781 cgggcagcga ctcaccggtg agcgccgatt cgtcggcctc caactcggcc agctcgacga
      841 ggcggaggtc cgcgggcacc ttgtcgcctg cctcgagcgc cacgacgtca cccgggacca
      901 gctcctccga tcggacggtc tgtagacgcc cgtcgcgccg cacccgtgcg tcggtgcgca
      961 ccatcgaacg caacacgtcc cgggcggcct cggccttcga ctcctgcacg aagccgacga
     1021 acgcattgac gaggacgaca ccgaagatca cggccgagtc gacacggtca cccaacagca
     1081 acgtgatgac gcccgcggcc aacaggacat agatgagcgg gtgatggaat tggcgcagga
     1141 aacgacgaac ggcacccgcg gcgtcggtct ccgccaacac gttcggcccg aagcggcgaa
     1201 gccgggcctc ggcctcggcg gtcgtcaacc cggccgcgac gtcggtcccg agcagcaaca
     1261 ccacctcgtg caccgcgacg gcatgatgcg cgcggccgcc ggagatcggt gcgcgatcgg
     1321 tatcgacagc gccgtgaccc tcgcgttcac ggaacgactt gtgcgacgcg gtcgggtcca
     1381 tcgcagaacg ccctatacgg cagccgactc gccgtacgcc ggtacccgtg ccggccagtc
     1441 cagctccagg tggatccggc ggcggagggt gtcggcggca ctcgcttcgc cgtgcacgac
     1501 gctcactccg tccggcgcag ccggcgcggc gcgcagccag gccatcagct cgtcgccgtc
     1561 cgcgtgcgcg gacaggcttt cgagctgcac gactcttgca cgcacaggga cgttctcgcc
     1621 gaacacccgt aacgtgcgcg ccccgttcgc gagcgcctct ccccgtgtgc ccgcggcctg
     1681 ataaccggcg agcacgatcg tgctgcgatg gtcgggcgcg acctggaaga ggtggtgcag
     1741 cacccttcct ccggcgagca tcccgctcgc cgacagcacg atcatcggtc ccggtcgcga
     1801 ggtcaaggcg atcgactcct ccacggtgcg taccagctcg acgttcttcc ccatctcgac
     1861 gacctcggcc ggcgtgaggc ggtgctgtcc ttctgaacgg atgaagagct cggtcgcgtc
     1921 gatcgccatc gggctgttca gatacgtcgg catctccggg atccggccgg catcccgcag
     1981 ccgcgccaac aggtgcagga ccgtctgcgc tcgcccgacg gcgaacaccg gaacgagcac
     2041 gatcccgccg cgtcgggccg tcgacgtgac cacctcggcg agcacgtcca tcggatcctc
     2101 gtccggatgc tgccgattgc cgtacgtcga ctcgacgacc acgtgatccg cggccaatgg
     2161 tgcggccggc gggcgcatga cccagtcgtc gctgcgaccc aagtcgccgc tgaagagcac
     2221 ggatcggttc ccgtccgaca gccacaccga cgcggccccc aggatgtggc ccgcggggct
     2281 gaaccgcagt ctcacaccgg gtgccacctc gacgtcgccg gcaccgtcgt gcgggcgga
//
LOCUS       DASUGA010000120         4033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_328376, whole genome shotgun sequence.
ACCESSION   DASUGA010000120 DASUGA010000000
VERSION     DASUGA010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328376"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VFX21_02290"
     CDS             <1..708
                     /locus_tag="VFX21_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5094809.1"
                     /translation="SVLVMVVFPVITIAFLAPAFKPALQQLGFVHANGADHVVPGQAV
                     MAAFFVVSLVVFSFFSEHAWQTWDRVRASCASPIEIVVGKALPRVVLVVAQVVLLLVV
                     GTGLFDMHVSGTAIALAPLIVVFALCLVMLGVCVTALARTAQQANAFSFLGMVLFGAL
                     GGAFVPFGLLPAWAKPIGPVTPTYWAMRGMRSVVLEQGAFGAITLPTAVLLGMSALFA
                     GIAAWRLRFDEAKVGWA"
     gene            complement(730..1326)
                     /locus_tag="VFX21_02295"
     CDS             complement(730..1326)
                     /locus_tag="VFX21_02295"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5094810.1"
                     /translation="MRTVFNLDMSMMIAIHDAFRRDLELVARMTGRSDGWDRFEKLLH
                     LHHRAEDDALWPVLRDALADQPEDLAFVDEMESQHAALGPLVAAIDASFERGEPAPLV
                     RAELDTLLRAHLADEEARALPLIDRTLSADEWMQFGAASVAIIGPAMPLFLPWLLDGA
                     DTDFVDSVLGRMPEAAQVTYWSEWQPAYAAKQWWDPNR"
     gene            complement(1348..2196)
                     /locus_tag="VFX21_02300"
     CDS             complement(1348..2196)
                     /locus_tag="VFX21_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5094811.1"
                     /translation="MTTTDTRSTLTKSEPVIAHATGAGLVSASLQSAKRTVLQFFRTP
                     QLLMLGTVQGALFLFMFRYIFGGAIDPGHGLDYVDFLVPGFLVTGILWVGMPASSGVA
                     EDAAGGVHDRLRSLPIPRSSVLFGRSIADTALTLWGMLVAGVLAFIVGFRFDAGALDV
                     VLALALLVIAAYCFAWVFITIGLVSGNAQAANGMATLLVIPVAFISAAYVPAQSLPGW
                     LQPVADHQPFTVFSNALRSLVLGGTDAVGLGHSTAYWVGLSLLWCAGILLLFATISIA
                     RFARRR"
     gene            complement(2211..3191)
                     /locus_tag="VFX21_02305"
     CDS             complement(2211..3191)
                     /locus_tag="VFX21_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007266117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5094812.1"
                     /translation="MTPIIEANGLTKRFGDVRALDELSLVAASGHVTALLGPNGAGKT
                     TFVSAVATLLLPDAGEIRVAGIDARAEPKRVREVIGLAGQYASVEPAMTGSENLRMVA
                     RLFGLGRRDAATAAASVLERLDLGNAGDRLVRTYSGGMRRRLDLGASLVGRPQLLLLD
                     EPTTGLDPRGRMELWSAIQDLVADGTDVLLTTQYLEEADQLARHVVIVDHGRVITEGT
                     PDELKDRAGANVIEVRPRSGGDLPAVEETLARIGGETPHTDIDSQRVWARVEGGGDQL
                     RDVVRVLDERGIEVDDVGLRRPSLDEVFLAVTGKSLEPTPNDLSAATDAA"
     gene            3349..>4033
                     /locus_tag="VFX21_02310"
     CDS             3349..>4033
                     /locus_tag="VFX21_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5094813.1"
                     /translation="MSTAAFDPRIGDVARDERRHLVDVAFRMLGDLSRAEDMVQEAFV
                     RLARSDLDSINDVRGWLVVVVGRLCLDHLRSASARREAASEQAAIEIASLASASATTD
                     GADPADRVTLDDSVRDALLAVLEHLTPAERTAFVLHDVFQFPFDTIGSIVGRTPTACR
                     QLASRARQRLRDESVPVRSHIESAQHHVVVERFMAAAAGGDIGALMEVLDPEVAGDGT
                     PGGVITAAGP"
BASE COUNT          626 a         1358 c         1386 g          663 t
ORIGIN      
        1 tcggtgctcg tgatggtcgt cttccccgtc atcacgatcg cgttcctcgc accggccttc
       61 aagccggcgc tgcagcagct cgggttcgtg cacgcgaacg gcgcggacca cgtcgttccc
      121 ggtcaagcgg tgatggcggc gttcttcgtc gtttcgctgg ttgtgttctc gttcttcagt
      181 gagcacgcgt ggcaaacctg ggatcgagtc cgggcgagct gtgcgtcacc gatcgagatc
      241 gtcgtcggga aagcgctccc tcgtgtcgtt ctcgtcgtcg cccaggtggt gctcctcctg
      301 gtcgtcggca cggggctgtt cgacatgcat gtcagcggca ctgcgatcgc gctcgcgccg
      361 ctcatcgtcg tcttcgctct ctgtctcgtc atgctcggcg tgtgcgtgac cgcgctggct
      421 cgcactgcgc agcaggccaa cgcgttctcg ttcctcggaa tggtgctctt cggcgcgctc
      481 ggtggcgcgt tcgtgccgtt cggcttgctg cccgcgtggg cgaagccaat cggaccggtg
      541 acgccgacct actgggcgat gcggggcatg cgctcggttg tgctggagca gggagcgttc
      601 ggcgcgatca cgcttccgac cgcggtgctg ctcggcatga gcgcgctttt cgcagggatc
      661 gcggcgtggc gcctgcgctt cgacgaagcg aaagtcggct gggcctgacc tctgctccgc
      721 acccaaccgt caccgattcg ggtcccacca ctgcttggcc gcgtatgcgg gttgccactc
      781 gctccagtag gtgacctgcg ccgcctcggg catgcgaccg aggacggagt ccacgaagtc
      841 ggtatcggct ccgtccagca gccatgggag gaagagcggc atggcgggac cgatgatcgc
      901 caccgacgcg gccccgaact gcatccactc gtccgcgctg agcgttcggt cgatgagtgg
      961 caacgcgcgc gcttcctcgt cggcgaggtg cgcccggagc agcgtgtcga gctcggcccg
     1021 aaccagcggc gcaggctccc cgcgctcgaa ggacgcgtcg atcgctgcga cgagcggccc
     1081 gagggcggcg tgttgcgact ccatctcgtc gacgaacgcg agatcttcgg gctgatccgc
     1141 gagcgcgtcg cgcagcacgg gccacagcgc gtcgtcttcg gcgcggtggt ggaggtgcaa
     1201 caacttctcg aaccggtccc atccgtcgga gcgtccggtc atcctcgcga cgagctcgag
     1261 atcacggcgg aacgcgtcgt ggatcgcgat catcatcgac atgtcgagat tgaagacagt
     1321 gcgcatgagt ttgcttcctt tccttcgtta gcggcggcgc gcgaagcgcg cgatggagat
     1381 cgttgcgaac aggaggagga tccccgcgca ccagagcagg ctcagcccga cccagtacgc
     1441 ggtgctgtgg ccgagtccga cggcgtcggt gccgccgagc accaggctgc gcaacgcgtt
     1501 gctgaacacg gtgaacggtt gatgatcggc aaccggctgc agccatcccg ggagcgactg
     1561 ggccggcacg tatgccgccg agatgaaggc gaccgggatg accaacaagg tggccatgcc
     1621 gttggccgct tgcgcgttcc cggagacgag accgatggtg atgaacaccc acgcgaagca
     1681 gtacgccgcg atcaccaaca acgcgagtgc gagcacgacg tcgagcgcgc cggcgtcgaa
     1741 ccggaatccg acgatgaacg cgaggacgcc ggcgacgagc atcccccaca gcgtgagtgc
     1801 ggtgtcggcg atcgaacggc cgaacagcac cgacgatcgc gggatcggca gcgagcgcag
     1861 ccgatcgtgc acgccgccgg ccgcatcttc cgcgacgccc gagctggccg gcatcccgac
     1921 ccagaggatc ccggtcacga ggaaccccgg aacgaggaaa tcgacgtagt cgagaccgtg
     1981 gccggggtcg atcgcgccac cgaagatgta acggaacatg aagaggaaca gcgcgccctg
     2041 cacggtgccc aacatgagca gttgcggcgt gcggaagaac tgcaggacgg ttcgcttcgc
     2101 gctctgcaga gatgcgctca cgaggccggc gccggtcgcg tgcgcgatta ccggctcact
     2161 cttggtcaga gtgctacgag tgtcggtcgt cgtcatgggt tcctcgaatg tcaggcggcg
     2221 tcagtcgccg cggagaggtc attcggcgtg ggctcgaggc tcttgccggt cacggcgagg
     2281 aacacctcgt cgaggctcgg acggcgtaga ccgacgtcgt cgacctcgat gccgcgctcg
     2341 tcgagcacgc gcacgacgtc gcggagctga tcgccaccac cctcgacgcg agcccagacg
     2401 cgctggctgt cgatgtcggt gtgtggtgtt tcgccgccga tgcgcgccag cgtctcttcg
     2461 acggccggga gatcgccgcc ggagcgcggc cgcacctcga tcacattcgc gccggcccgg
     2521 tctttcagct cgtccggtgt tccctcggtg atcacgcgac cgtggtcgac gatgacgacg
     2581 tgacgggcga gttgatcggc ttcctccagg tattgcgtcg tgagcagcac gtcggtgccg
     2641 tcggcgacga gatcctggat cgcgctccac agctccatgc ggccgcgtgg atcgagacct
     2701 gtcgtcggtt cgtcgagcag gagcaactgg ggccggccga ccaagctcgc gccgaggtcg
     2761 agacggcgcc gcatgccacc cgaataggtg cgcacgagac ggtcgccggc attgccgaga
     2821 tccagtcgtt cgagcacgct cgcagccgcg gtggccgcgt cgcgcctgcc gaggccgaac
     2881 agtcgcgcca ccatgcggag gttctcgctg cccgtcatcg cgggttcgac cgacgcgtac
     2941 tggccggcca gtccgatgac ttcgcgcacc cgcttgggct cggcccgggc gtcgatgccc
     3001 gcgacgcgaa tctcgcccgc gtccgggagc aacaaggttg cgaccgcgct gacgaacgtg
     3061 gtcttcccgg cgccgttggg gcccaggagc gcggtgacgt ggccggacgc ggcgacgagg
     3121 ctcagttcgt cgagcgcacg aacgtcacca aaacgcttcg tcagaccgtt ggcctcgatg
     3181 atcggagtca cgctatgtcc ctcctcggaa ccgggtaccg aacacccgac agtcagagga
     3241 cgggcatggc catcggaacg tgacgcggaa tcgacgtcac atccggaacg gctgtctcgt
     3301 cctctgatcg tgtcctgcgc gcgtggcgcg ccgacgagag gagtgtccat gtcgacagcc
     3361 gcgttcgacc cccggatcgg cgacgtcgcg cgcgacgagc gccggcatct cgtcgacgtc
     3421 gcgtttcgga tgctcggcga cctgagccgg gccgaggaca tggtgcagga ggcgttcgtt
     3481 cgactcgccc gcagtgatct cgacagcatc aacgacgtcc gcggttggct cgtggtcgtg
     3541 gtcggacgcc tctgtctcga ccacctccgg tccgcgagcg cgcgccgcga agccgcgagt
     3601 gagcaagccg cgatcgagat cgcgagtctc gcgtcggcgt cagcgacgac cgatggcgcc
     3661 gacccggccg atcgcgtgac gctcgacgac agcgtgcgcg acgcgctcct cgcggtgctc
     3721 gagcacctca cacccgccga gcgcaccgcg ttcgtgctgc acgacgtctt tcaattcccg
     3781 ttcgacacca tcggctcgat cgtcggtcga acacccaccg catgccgcca gctcgcgagc
     3841 cgggcgcgtc agcgtctgcg cgacgagtcg gtaccggtcc ggtcgcacat cgagtccgcg
     3901 cagcaccacg tcgtcgtgga acggttcatg gccgccgccg cgggcggcga catcggcgcg
     3961 ctcatggaag tgctcgatcc cgaggtcgcg ggcgacggca caccgggcgg cgtcatcacc
     4021 gccgcgggac ctc
//
LOCUS       DASUGA010000121         2716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1094683, whole genome shotgun sequence.
ACCESSION   DASUGA010000121 DASUGA010000000
VERSION     DASUGA010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094683"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="VFX21_02315"
     CDS             <1..1091
                     /locus_tag="VFX21_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HEX5094814.1"
                     /translation="AHARHTIAEHGDDAPIAWVHRDDRFGEKLATPDTSIADLIGEVD
                     PIKVAEGRYLSDELTLHYGLVPRTNRGIFAINELPDLAERIQVGLLNVLEERDVQIRG
                     YKIRLPLDVLLFASANPEDYTNRGRIITPLKDRFGAQIRTHYPLDVETEVAIARQEAR
                     PLEADGVRVVVPQYMADVVAQLSQLARESPHINQRSGVSVRLTVSNLETLVANGLRRS
                     LVHGESDVVPRVSDLDALASSTAGKVEIESIDEGRDEQVVEHILKSAILTTFRDSLSV
                     EDLRAVVDAFEGSRVVHAGEDVSSAEYVALLEDMKALREPVLRLTGGDESAASIASAV
                     EFILEGLHLSKRLNKEAVGARAQYRGRG"
     gene            complement(1125..1787)
                     /gene="rpiA"
                     /locus_tag="VFX21_02320"
     CDS             complement(1125..1787)
                     /gene="rpiA"
                     /locus_tag="VFX21_02320"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011749904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEX5094815.1"
                     /translation="MTDGVDAWKEAAGRQAAEFVVDGMRVGLGTGSTVHWTIVALGER
                     RPDIVCAATSKQTAELATSLGLQVAPADELGSLDIAIDGADEVDPAYNLTKGGGGAQT
                     REKIIATMARRFVVVVDEHKLVPRLGPFGTPVELLPFAPKVAMAWIRDLGAQSVEARR
                     EPSDNGNVLADAHFGAIEAPEELAQQLAAVPGLVEHGLFPAALVERVIVGGPSGVREL
                     VR"
     gene            complement(1784..>2716)
                     /locus_tag="VFX21_02325"
     CDS             complement(1784..>2716)
                     /locus_tag="VFX21_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEX5094816.1"
                     /translation="HFTLPGWFSDDEGGFVDERARQYYWARHVDWVGETFGDLVFGWK
                     PINEPIFYALMGYLIGTIPPGHASVTEFDAALEAIHLASIDAARLLHSGGQPVATIDG
                     LMPIHPVTRTRDPNEREQANEMAEQFRARLWCGVRAMRDGILAVPGRKPIEVPEATTS
                     FDSAGFSYYAALGLHADGSSTSYPADAEVGPMGYAPWPEGLGVVLRELAEELPGRRIL
                     VDECGLGTSRDPETGRDRGDDKRVTYLSECISHVERAIDDGIDVAGFFHWTAVDNYEW
                     LHGYDVLFGLFDRDRNAKSSAALAARFAGKGAAA"
BASE COUNT          425 a          944 c          926 g          421 t
ORIGIN      
        1 cggcccacgc ccgccacacc atcgccgagc acggcgacga cgcaccgatc gcctgggtgc
       61 accgcgacga ccgcttcggc gagaagctcg ccacccccga cacgtcgatc gccgacctca
      121 tcggcgaggt cgacccgatc aaggtggccg agggtcggta cctgtccgac gagctgacgt
      181 tgcactacgg cctggtgccg cgcacgaacc gcggcatctt cgccatcaac gagctgcccg
      241 acctggccga gcgcatccag gtcggcttgc tcaacgtgct cgaggagcgc gacgtccaga
      301 tccgtggcta caagatccgc ctcccgctcg acgtgctgct gttcgcgtcg gccaacccgg
      361 aggactacac caaccgcggc cgcatcatca cgccgctgaa ggatcgcttc ggcgcacaga
      421 tccgcacgca ctatccgctc gacgtcgaga ccgaggttgc gatcgcacgc caagaggcgc
      481 ggccgttgga ggccgacggc gtccgcgtgg tcgttccgca gtacatggcc gatgtcgtgg
      541 cccagctcag ccagctcgcg cgcgagagtc cccacatcaa ccagcgctcg ggcgtgtcgg
      601 tgcggctcac ggtgtcgaac ctcgagacgc tggttgccaa cggcttgcgt cgttcgctcg
      661 tgcacggtga gagcgacgtc gtcccgcgcg taagcgacct cgacgcgttg gcgtcgtcga
      721 ccgccggcaa ggtcgagatc gaatcgatcg acgaaggtcg cgacgagcag gtggtcgagc
      781 acatcctcaa gtcggcgatc ctcaccacgt tccgcgattc actgtcggtc gaagacctgc
      841 gcgcggtggt cgatgcgttc gagggcagcc gggtggtgca cgccggggag gacgtgagct
      901 ccgcggagta cgtggcgctg ctcgaggaca tgaaggcgct gcgcgagccc gtgctgcggc
      961 tcactggcgg tgacgagtcg gctgcatcca tcgcgagcgc ggtcgagttc atcctcgaag
     1021 gcctccacct gtcgaagcga ctcaacaagg aagccgtcgg cgcgcgagcg cagtaccgcg
     1081 gtcgcggcta gcgctggctc acacctgtcg cgacgggcga acggtcagcg aaccagctcg
     1141 cgcacgcctg acggaccacc gacgatcacg cgctccacga gcgcggcggg gaacagcccg
     1201 tgttcgacca gccccggcac cgcggccagt tgctgggcga gctcctcggg cgcttcgatc
     1261 gcaccgaagt gcgcgtcggc gagcacgttg ccgttgtcgc tcggctcgcg gcgcgcctcc
     1321 accgactgtg cgccgagatc gcggatccat gccatggcca ccttgggtgc gaacgggagc
     1381 agctccactg gtgtgccaaa cgggccgaga cggggcacga gcttgtgctc gtcgaccacg
     1441 acgacgaacc ggcgcgccat cgtggcgatg atcttctccc gggtctgcgc gccgccgcct
     1501 cccttggtga ggttgtaggc ggggtcgacc tcgtcggcgc cgtcgatggc gatgtcgagc
     1561 gagcccagct cgtccgccgg tgcaacctgc aagccgagcg atgtcgcgag ctcggccgtc
     1621 tgtttcgacg tggccgcgca cacgatgtcg gggcgacgct caccgagcgc cacgatcgtc
     1681 cagtggaccg tggagccggt gcccagcccg acgcgcatcc cgtccacgac gaactcagcg
     1741 gcctgtcgtc cggccgcttc cttccaggcg tcgacgccgt cggtcatgct gctgctcctt
     1801 tgccggcgaa ccgcgccgcc agcgcggcgc tcgatttggc gttgcggtcg cgatcgaaca
     1861 ggccgaacag cacgtcgtag ccgtgcagcc attcgtagtt gtccaccgcc gtccaatgga
     1921 agaacccggc gacgtcgatc ccgtcgtcga tggcccgctc gacgtgggag atgcattcgg
     1981 acaggtaggt gacacgcttg tcgtcgccgc ggtcacgacc ggtctccggg tcgcgcgagg
     2041 ttccgagccc gcattcgtca accaggatgc gacggccggg tagctcctcg gcgagctcgc
     2101 gcagcaccac cccgagccct tcgggccagg gcgcgtagcc catgggtccc acctcggcgt
     2161 cggccggata gctcgtgctc gatccgtcgg cgtggaggcc gagcgcggcg tagtacgaga
     2221 agccggcaga atcgaagctg gtcgtcgcct ccggtacctc gatcggcttg cgcccgggca
     2281 cggccaggat gccgtcgcgc atggcgcgca cgccgcacca gagccgggcg cggaactgct
     2341 cggccatctc gttcgcttgc tcgcgctcgt tcgggtcgcg tgtgcgcgtg accggatgga
     2401 tgggcatcag gccgtcgatg gtggccaccg gttgcccgcc gctgtgcaga aggcgagccg
     2461 cgtcgatcga cgcaagatgg atggcttcga gcgccgcgtc gaactcggtc acgctggcgt
     2521 ggccaggcgg gatggtgccg atcaggtagc ccatcagcgc gtagaagatc ggctcgttga
     2581 tcggcttcca gccgaagacg agatcgccga aggtctcgcc cacccagtcg acgtgacgag
     2641 cccagtagta ctgccgggcg cgctcgtcga cgaacccgcc ctcgtcgtcg ctgaaccaac
     2701 cgggcagggt gaagtg
//
LOCUS       DASUGA010000122         2447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1699823, whole genome shotgun sequence.
ACCESSION   DASUGA010000122 DASUGA010000000
VERSION     DASUGA010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699823"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /gene="folP"
                     /locus_tag="VFX21_02330"
     CDS             complement(<1..439)
                     /gene="folP"
                     /locus_tag="VFX21_02330"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX5094817.1"
                     /translation="MGIVNVTPDSFSDGGQFLDADRAIAHGLALAAAGAAVLDVGGES
                     TRPGAEPVSANDEIARVLPVVTALARDADVPVSIDTAKAEVAAAALEAGARMVNDVTG
                     GADPAMLPLVAGERAAYVAMHMRGTPRSMQQQTDYGDVVRDVIT"
     gene            complement(507..1115)
                     /gene="folE"
                     /locus_tag="VFX21_02335"
     CDS             complement(507..1115)
                     /gene="folE"
                     /locus_tag="VFX21_02335"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017622676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX5094818.1"
                     /translation="MSLPDGVEPDGIDLGRIEKAVREILLAVGENPDRDGLQRTPRRV
                     AEMYAEIFSGLHEDPARHLVVTFEANHDEMVMVRDIPLYSMCEHHLATFHGRAHVAYI
                     PGPDGRITGLSKLARLVDGYAKRPQVQERLTTQIADAIVETLQPSGAFVMIEAEHLCM
                     SMRGVRKPGALTVTSAVRGLFKDNPATRAEVMALLGPTVGRS"
     gene            complement(1116..1652)
                     /gene="hpt"
                     /locus_tag="VFX21_02340"
     CDS             complement(1116..1652)
                     /gene="hpt"
                     /locus_tag="VFX21_02340"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX5094819.1"
                     /translation="MKDPNIGRVVVDEHELQQRIKDLGKEIGADYEGRPPLLVGILKG
                     AFMFMADLARAIDLPVEFDFMAVSSYGSATRTSGVVRILKDLDLDLTDRHVLIVEDIV
                     DSGLTLAYLRKNLKARGPASLEVCALLVREGLQKTEQDLKYVGFRIPPKFVIGYGLDV
                     DERYRNLPYVAEYDGPEL"
     gene            complement(1649..>2447)
                     /gene="tilS"
                     /locus_tag="VFX21_02345"
     CDS             complement(1649..>2447)
                     /gene="tilS"
                     /locus_tag="VFX21_02345"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX5094820.1"
                     /translation="LCRALGLAPVQDPMNDDTHFRRVWLRREVIPMLERGARSDLVDV
                     LARQAELVRADSALLDDLAAAHTIDDIATLPDAMARRVVRQWLQHPVPDAATIERVLA
                     VGRGERAATEVPGGDRIERVRGRLTRVARTEPPAPAPLALPGATRFGPVAIEAWIEHA
                     PPVSWPDGRRVAVCDADLVRASAVVRAPALGERFQPLGGPGSKLVHDALAEAGVPAAL
                     RAASPVVVAESPVWVVGYRIDHRVRVTAHTRRYLWLSAEPVQAMSDV"
BASE COUNT          410 a          842 c          820 g          375 t
ORIGIN      
        1 cggtgatgac gtcgcgtacg acatcgccat aatccgtctg ctgctgcatc gagcgcggcg
       61 ttccgcgcat gtgcatcgcg acgtacgccg cgcgctcgcc cgcgaccagc ggcagcatcg
      121 cgggatcggc gccgccagtg acgtcgttca ccatgcgcgc accggcttcg agcgcggctg
      181 ccgcgacctc ggccttcgcg gtgtcgatac tcacggggac gtccgcgtcg cgcgccagcg
      241 cggtgacgac cggcagcacg cgcgcgattt catcgttggc ggaaaccggc tcggcgcccg
      301 gacgcgtgga ctcgccgccc acgtcgagca cagcagcacc ggccgcggcg agcgccaacc
      361 cgtgcgcgat cgcgcgatcg gcgtccagaa attggccgcc gtcggagaac gaatcaggtg
      421 tgacgttgac aatccccatc agcgcccgtc gcgggagatc agcgacgcgg agcagcggtg
      481 cggtcacaga cgggacacta ctgcggtcag gagcggccga cagtaggtcc gagcagggcc
      541 atgacctcgg cgcgcgtcgc cgggttgtcc ttgaagaggc cgcgcacggc ggacgtcacg
      601 gtgagcgcgc cgggtttgcg cacaccgcgc attgacatgc agaggtgttc ggcttcgatc
      661 atcacgaacg cgccgctcgg ctgcagggtc tcaacgatcg cgtcggcaat ctgcgtcgtg
      721 agccgctcct gcacctgcgg ccgcttggcg tagccgtcga cgagccgcgc cagtttcgaa
      781 agaccggtga tccggccatc gggtcccggg atatacgcaa cgtgcgcacg tccgtggaac
      841 gtggcgaggt ggtgctcgca cattgaatac agcgggatgt cccgaaccat gaccatctcg
      901 tcgtggttcg cttcgaacgt cacgaccaag tggcgcgccg gatcctcgtg caagccgctg
      961 aagatctccg cgtacatctc ggcaacgcgg cggggcgtgc gttgcaagcc gtcacggtcg
     1021 gggttttcac cgaccgcaag caagatctcg cgcactgcct tttcgatacg cccgaggtcg
     1081 atgccgtcgg gctccactcc gtcaggaagg ctcacttaca actccggacc gtcgtactcc
     1141 gcgacgtacg gcaggtttcg atacctctcg tcgacgtcga ggccgtagcc gatgacgaac
     1201 ttgggcggga tgcgaaaccc gacgtacttc aagtcttgtt ccgtcttctg caacccttcg
     1261 cgcacgagca acgcgcagac ttcgagcgaa gccggcccgc gcgccttcag gttcttgcgc
     1321 agatacgcga gcgtgagacc cgagtcgacg atgtcttcga cgatcaagac gtggcggtcg
     1381 gtgagatcga gatcgaggtc cttcaagatc cgcacgacgc cactggtgcg cgtggccgat
     1441 ccgtacgacg acaccgccat gaagtcgaac tcgaccggca agtcgatcgc ccgtgcgagg
     1501 tcggccatga acatgaacgc acccttgaga atgccgacga gcagcggcgg ccggccttcg
     1561 tagtcggcgc caatttcctt gccgagatcc ttgatgcgtt gctgcagctc gtgctcgtcg
     1621 acgacgacac ggccgatgtt cggatctttc atacgtcaga catcgcttgc acaggctccg
     1681 cgctcagcca gaggtaacgc cgcgtgtggg ccgtgacccg gacgcgatgg tcgatacggt
     1741 aaccgacaac ccaaaccggg ctctcagcga cgacgaccgg gctggccgca cggagcgcgg
     1801 cggggacgcc ggcctcggcg agcgcgtcat gcacgagctt cgatcccggc ccgccgaggg
     1861 gctggaagcg ctcacccaga gccggcgccc gcacgactgc ggacgctcgg acgagatcgg
     1921 catcgcagac cgcgacgcgg cggccgtcgg gccacgacac cggaggcgcg tgctcgatcc
     1981 acgcttcgat cgcgaccggg ccgaagcgcg ttgcacccgg cagcgccaac ggtgccggcg
     2041 ccggcggttc cgtgcgcgca acgcgcgtga ggcggcctcg cacgcgttcg atgcgatcac
     2101 cgccgggcac ctcggtcgcc gcccgttcgc cgcgaccgac cgcgagcacg cgttcgatcg
     2161 tcgcggcatc gggcaccgga tgctgcagcc actgccgcac cacgcggcgc gccatcgcgt
     2221 cgggaagcgt cgcgatgtcg tcgatggtgt gcgcggccgc caagtcgtcg agcagcgcgc
     2281 tgtcggctcg cacgagctcg gcctggcgcg caagcacgtc gacgagatca ctgcgcgcgc
     2341 cgcgttcgag catcgggatg acctcgcggc gcaaccacac ccgccggaag tgcgtgtcgt
     2401 cgttcatcgg atcctgcacc ggcgcgagcc cgagcgcgcg acagagt
//
LOCUS       DASUGA010000123         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1232726, whole genome shotgun sequence.
ACCESSION   DASUGA010000123 DASUGA010000000
VERSION     DASUGA010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232726"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VFX21_02350"
     CDS             complement(<1..347)
                     /locus_tag="VFX21_02350"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5094821.1"
                     /translation="MPYDVLITGGRIVDGTGRDAFEGNVAIEAGRVVGVGDVGGQAKR
                     MIDADGHVVAPGFIDAHTHYDAQLLWDASANPSTAHGITTILMGNCGYTLAPVRSDDQ
                     DYLIGLFAAAEEVP"
     gene            complement(379..1737)
                     /locus_tag="VFX21_02355"
     CDS             complement(379..1737)
                     /locus_tag="VFX21_02355"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX5094822.1"
                     /translation="MAAHASVTEQLAEWIVTADVADVPPAGIQRVRDRVLDSLGVMFA
                     GMSVSTGQLMRDWIRAQGGTPDCTVVASGFKTTASFATLANSSAGHALEFDDIATFGG
                     HFANPLTAAALAVGEKVGASGRDTVLAWLVGWDVIAQTSKPCATPQGNSLLNRGWFNQ
                     GFQPTLGVAALASKLMGLDVLQTRMALGNAAGAMGGLLKNRGSDTKSFQAGNAAMHGV
                     MAAELVALGFTANPDILDGDIGVARLLGLEAGDAERALEGLGTWDMATNGSTIRLHAC
                     CGASHWGQDALVKILRQRPTVPDEIESIGIEIDEFLLPMVPYHDPQTGLEAKYSLEYD
                     MATIVLDGRAGLHQYSDDAVRRPEARALMERVTIHPVNEVRRPESRVVVTLKSGDQLD
                     ESVNVSHGSPNDPLTNAEILGKFHECAEALMPEAQRQQVIDLCGRLDSLASIRELTDV
                     IG"
     gene            complement(1772..>2119)
                     /locus_tag="VFX21_02360"
     CDS             complement(1772..>2119)
                     /locus_tag="VFX21_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HEX5094823.1"
                     /translation="DVLIAGRNFGVGSARPIGDVLRDNGISGVVADSFNGLGLRNCIN
                     AGLPSLPCAGILDAFDEGDVAEVDWTTGTVRNVTKGTTLQGSPVPEALQDIVKAGGVE
                     AVLRNEGYLAARS"
BASE COUNT          306 a          743 c          709 g          361 t
ORIGIN      
        1 ggcacctctt cggccgcggc gaacaaccca atcaagtagt cctggtcgtc cgatcgcacc
       61 ggcgccagcg tgtagccgca gttgcccatc aggatcgtgg tgatgccgtg cgcggtcgac
      121 gggttcgcgg acgcgtccca cagcagctgc gcgtcgtagt gcgtgtgcgc gtcgatgaag
      181 cccggagcga cgacgtggcc gtcggcgtcg atcatccgtt tcgcctggcc accgacatca
      241 ccgacgccga cgacccggcc cgcctcgatg gcaacgttgc cctcgaaggc gtcgcggccg
      301 gtcccgtcga cgatgcggcc gccggtgatg agcacgtcgt acggcacgga cctacctccc
      361 tcgatctctt ggctcggatc agccgatgac gtcggtgagc tcgcggatgc tggcgagcga
      421 gtcgagtcgg ccgcagaggt cgatgacctg ctgacgctgg gcttcgggca tgagcgcctc
      481 ggcacactcg tggaacttgc cgaggatctc cgcgttcgtc agcggatcat tcggcgagcc
      541 gtgtgacacg ttgacgctct cgtcgagctg gtcgccgctc ttcagcgtca ccacgacccg
      601 gctctcgggc cggcgcacct cgttcaccgg gtggatcgtg acccgctcca tgagcgcgcg
      661 cgcttcgggc cgccgcaccg cgtcgtcgct gtactggtgc aggccggcgc gcccgtcgag
      721 cacgatcgtg gccatgtcgt actcgaggct gtacttcgct tcgagcccgg tctgcgggtc
      781 gtggtacggg accatcggca gcaggaactc gtcgatctcg ataccgatcg actcgatctc
      841 gtcgggaacc gtcgggcgct gccggaggat cttcacgagt gcgtcctgac cccagtggct
      901 cgcgccgcaa cacgcgtgga gccggatcgt cgaaccgttc gtggccatgt cccaggtgcc
      961 gaggccctcg agcgcgcgct cggcatcgcc ggcttcgagc ccgagcaggc gcgccacgcc
     1021 gatgtcgccg tcgaggatgt cggggttggc ggtgaacccg agcgccacga gctcggccgc
     1081 catgacaccg tgcatcgccg cgttccccgc ctggaacgac ttcgtgtcac tgccgcggtt
     1141 cttgagcagt ccgcccatcg cgcccgcggc gttgccgagt gccatgcgcg tctgcaggac
     1201 gtcgagcccc atgagcttgc ttgcgagcgc ggcgaccccg agcgtgggct ggaagccctg
     1261 gttgaaccaa ccgcggttga gcaagctgtt gccttgtggc gttgcgcacg gcttcgacgt
     1321 ctgcgcgatg acatcccaac cgaccagcca cgcgagcacg gtgtcgcgac ccgacgcgcc
     1381 gaccttctcc ccgaccgcga gcgccgccgc ggtgagcggg ttggcgaagt gccccccaaa
     1441 ggtcgcgatg tcgtcgaact cgagcgcgtg accggcgctc gagttggcga gcgtcgcgaa
     1501 cgacgccgtc gtcttgaacc cgctcgcgac gacggtgcag tcgggagtcc cgccttgcgc
     1561 cctgatccag tcacgcatca gctgaccggt cgacaccgac atgccggcga acatcacgcc
     1621 caacgaatcg agcacgcggt cgcgcacacg ctggatgcct gccggtggca cgtcggcgac
     1681 atccgccgtg acgatccact ccgcgagctg ttcggtcacc gatgcgtgag cggccatggg
     1741 tgcttcctct tctctctgac tactgctcgg tctacgaacg ggcggcgagg tagccctcgt
     1801 tgcgcaggac tgcttcgacg ccccccgcct tgacgatgtc ctgcagcgcc tcggggacgg
     1861 ggctgccctg gagcgtcgtg cccttcgtga cgttgcgcac ggtgccggtg gtccagtcga
     1921 cttcggcgac gtcaccctcg tcgaacgcgt cgaggatccc ggcgcatggc agcgacggca
     1981 atcccgcgtt gatgcagttg cgcaggccga ggccgttgaa cgagtcggcg acgactccgc
     2041 tgatcccgtt gtcgcgcagg acgtcaccga tgggccgcgc cgatccgaca ccgaagttgc
     2101 ggcccgcgat cagcacgtc
//
LOCUS       DASUGA010000124         3681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1023335, whole genome shotgun sequence.
ACCESSION   DASUGA010000124 DASUGA010000000
VERSION     DASUGA010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023335"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VFX21_02365"
     CDS             <1..343
                     /locus_tag="VFX21_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX5094824.1"
                     /translation="EETLMEIWSLTRVPDGTERDKPMPPEPWAPDDARWPPIPRQDFS
                     NLPRQQRGLHAKGFEYMRLSEMAEGHISNFHRTIDLFLAGTPTEQLLRPLREINLNPL
                     ERPIVDIETGP"
     gene            340..732
                     /locus_tag="VFX21_02370"
     CDS             340..732
                     /locus_tag="VFX21_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5094825.1"
                     /translation="MTTYAEVVEGVRATIAWYTHALDDGRTDDIVATFCPDGTIEIPG
                     MGTHSGHDALRAAYAKWVPRQPQRHLVVNTLVRGWNEHEANAVSDVVFLLKGDTGWAV
                     QVVGRYRDVLHNEDGVWRFHHRAAEFVT"
     gene            737..1030
                     /locus_tag="VFX21_02375"
     CDS             737..1030
                     /locus_tag="VFX21_02375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5094826.1"
                     /translation="MAEHTVVDNTELHRFETTVGNAVAFLDYALHGDRLTLVHTEVPE
                     ELEGHGVGSELVQFAIDFADEHAYTVIPRCPFARGWLERHPDVAARVTIEQPA"
     gene            1082..1498
                     /locus_tag="VFX21_02380"
     CDS             1082..1498
                     /locus_tag="VFX21_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5094827.1"
                     /translation="MTTTGPAPKSLPLILARELASNLATPMFILDGIGTLVFFNDAAE
                     MLLGKSYGEVGEITGNELGEMLQLRHQDGTPMRRRDSPAGVAFYERRPAHCITQVKML
                     DGTHRLVEATAYPLFGAGETLHGVVTVFWQIAQADG"
     gene            1498..2367
                     /locus_tag="VFX21_02385"
     CDS             1498..2367
                     /locus_tag="VFX21_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5094828.1"
                     /translation="MRARVWGCRGSLATPGETTIRYGGNTSCIEVRLDSGDVLVLDAG
                     TGMRPLGVALEEDPPAEIHVLLSHLHLDHVQGLGFFRPLFRPDAQVHIWGPASPVQSL
                     AERLAIYLSPPLFPVRLADIPAQLTFHDQPEADVPIGSGTIRAGSVTHQGPTVGWRIE
                     EHGRVLSYLPDHEPVIGVQIDDLGDDWLSGYDVARDADVLFHDAQYGDEEYPRHVGWG
                     HSSIEHVMGFARRARADTLVLFHHDPYHSDDDLETLLDDARRLTNGQVANVCLAHEGM
                     SIDIDKDGTRFVD"
     gene            complement(2410..2700)
                     /locus_tag="VFX21_02390"
     CDS             complement(2410..2700)
                     /locus_tag="VFX21_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094829.1"
                     /translation="MRKKVAIALGFSALFVGLVPSAAFAGEVNGAGTKHYFSQGKSAC
                     KFSGLNDTPDAPAPEGGRTQTYGQLVRQGLKADFPSPGVACNPNTPPPPEEA"
     gene            complement(2827..>3681)
                     /locus_tag="VFX21_02395"
     CDS             complement(2827..>3681)
                     /locus_tag="VFX21_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5094830.1"
                     /translation="PFTPGMESVGIVEAAGPGAEHLLGQRVVGIPVFAHGGYAEYALV
                     DAATTLHIPAEMDAADAAALHYPFHLGLFALEERARLQPEETLLVHAAAGGTGSGAVQ
                     IGKALGAKVIATAGSDEKLDVCRELGADVVINYRTQNFVDVVNEVTYGHGVDVAWDTI
                     GGDTTLETFKCMAMNGRHLILGFSADIQLEDDGYITPRAMAYGNFSLCGVCLVYVLDP
                     LGTRRTLGFNWPSRSEGQAAHRTILEMIRTGAIRPFVGRELAFDEIPDALEAMERRET
                     VGRLVVRM"
BASE COUNT          621 a         1266 c         1197 g          597 t
ORIGIN      
        1 cgaagaaacg ctcatggaga tctggtcgct gacgcgcgtc cccgacggca ccgagcgcga
       61 caaaccgatg ccacccgagc cgtgggcgcc cgacgacgcg cgctggccgc caatcccccg
      121 acaggacttc tcgaacctgc cgcgccagca gcgcggattg cacgcgaagg gcttcgagta
      181 catgcggttg tcggagatgg ccgaaggaca catctcgaac ttccaccgca cgatcgacct
      241 cttcctcgcg ggcacgccga ccgaacagct gctgcgtccg ctgcgcgaga tcaacctcaa
      301 tccgctggaa cgaccgatcg tcgacatcga aacgggacca tgaccaccta cgccgaggtc
      361 gtcgaaggcg tccgggccac gatcgcgtgg tacacgcacg ccctcgacga cggccgcacc
      421 gacgacatcg tcgcgacatt ctgtcccgat ggcaccatcg agattcccgg gatgggcacc
      481 cacagcggac acgacgcact gcgcgccgcg tacgcgaagt gggtgccccg tcaaccgcaa
      541 cggcatctcg tcgtgaacac gctcgtccgc gggtggaacg agcacgaagc gaacgcggtc
      601 agcgacgtcg tcttcctcct gaagggcgac acgggctggg cggtgcaagt cgtgggccgc
      661 taccgcgacg tgctgcacaa cgaagacggc gtctggcgtt tccaccatcg ggccgcggag
      721 ttcgtaacgt aggtccatgg ccgagcacac cgtcgtcgac aacaccgaac tccaccgctt
      781 cgagacgacg gtcggcaacg cggtcgcgtt cctcgactac gccttgcacg gcgatcggct
      841 cacactcgtc cacaccgaag tgcccgagga gctcgaaggc cacggcgtcg ggagtgagct
      901 cgtccagttc gcgatcgact tcgcggatga gcacgcgtac acggtcatcc ctcgttgccc
      961 gttcgcgcgc ggctggctcg aacggcatcc cgatgtcgcc gcccgcgtga ccatcgaaca
     1021 gcccgcatag cctcacgcag gcccctggtc aggcgcgcag gctgcggtta gcgtccgcgt
     1081 cgtgacgacg accgggcccg cgccgaagag cctgccgctc atcctcgccc gcgagctcgc
     1141 gtcgaacctc gcgacaccga tgttcattct cgacggcatc ggcaccctcg tgttcttcaa
     1201 cgacgccgcg gagatgctgc tcggcaagtc ctacggcgaa gtgggtgaga tcaccggcaa
     1261 cgagctcggc gagatgctgc aactgcgcca ccaagacggc acacccatgc ggcggcgcga
     1321 ctcgcccgcc ggcgtcgcgt tctacgagcg ccgtccggcg cactgcatca cgcaggtgaa
     1381 gatgctcgac gggacgcacc gcctcgtcga agcaaccgcg tatccgctct tcggtgcggg
     1441 tgagacgttg cacggtgtgg tgacagtgtt ctggcagatc gcacaggccg acggctgatg
     1501 cgcgcccggg tctggggttg tcgcggctca ctcgcgacac cgggcgagac cacgattcgc
     1561 tacggcggca acacctcgtg catcgaagtc cggctcgaca gcggcgatgt gctcgtgctc
     1621 gatgcgggca ccggcatgcg cccgctcggc gtcgcgctcg aggaagaccc gccggccgag
     1681 atccacgtgc tgctgagtca tttgcatctc gaccacgtgc agggactcgg cttcttccgt
     1741 ccgttgttcc ggcccgacgc gcaagtgcac atctggggtc cggcgtcgcc ggtgcagagc
     1801 ctcgccgaac ggctcgcgat ctacctgtcg ccgccgctgt tcccggtgcg gctcgccgac
     1861 atccccgcgc agttgacgtt ccacgaccag cccgaggccg acgtgccgat cggctcgggg
     1921 acgatccgcg cggggagcgt gacgcaccaa ggaccgacgg ttggttggcg tatcgaggag
     1981 cacggccgcg tgctctccta tctccccgat cacgaaccgg tgatcggcgt gcagatcgac
     2041 gatctcggcg acgactggct cagcggttac gacgtcgcgc gcgacgccga tgtgctgttc
     2101 catgacgcgc agtacggcga cgaggagtac ccgcgccatg tcgggtgggg tcattcgtcg
     2161 atcgagcacg tcatgggctt cgcccgccga gcgcgcgcgg acacgttggt cttgttccat
     2221 cacgatccgt atcacagcga cgacgatctc gagaccttgc tcgatgacgc gcggcgcctg
     2281 acgaacggac aggttgcgaa cgtgtgcctc gcccacgagg gcatgtcgat cgacatcgac
     2341 aaggacggca ctcgcttcgt cgattgacgt ccggttggcc tcaccgaggc caaccgaacc
     2401 gtcgatcgat caggcctctt cgggcggcgg cggtgtgttg gggttgcagg ccacacccgg
     2461 gctcgggaag tcggccttca ggccctggcg caccaactgc ccgtaggtct gcgtccggcc
     2521 gccctcgggc gcgggtgcgt ccggggtgtc gttcagaccg gagaacttgc aggctgactt
     2581 gccttgcgag aagtagtgct tcgtgcccgc gccgttgact tcaccggcga atgccgcact
     2641 cggcaccaga ccgacgaaca acgccgagaa cccgagcgcg atcgcgactt tcttcctcat
     2701 ccgtagttcc tctcactgca acgtcccgcc agccgccgag gaaggtatct ggaccacccc
     2761 gcgcgagcga cgccatctac ccgttttgcg aagtcgcgct gagttgcggt tgaagggtcg
     2821 acgcgttcac atgcgcacga cgagacgacc gaccgtttcg cgacgttcca tcgcttcgag
     2881 tgcgtcgggt atctcgtcga acgcgagctc gcggccgacg aatggtctga tggctccggt
     2941 gcggatcatc tcgaggatcg tccggtgcgc ggcttgtccc tcggagcgcg agggccagtt
     3001 gaacccgagt gtgcggcgag tgccgagcgg gtcgaggacg tagacgaggc acacgccgca
     3061 caggctgaag ttgccgtacg ccatggcgcg cggcgtgatg tagccatcgt cctcgagttg
     3121 gatgtcggcg ctgaacccga ggatcaggtg acgcccgttc atcgccatgc acttgaacgt
     3181 ctccagcgtc gtgtcaccgc cgatcgtgtc ccacgcgaca tcgacgccgt ggccgtaggt
     3241 cacctcgttg acgacgtcga cgaagttctg cgtgcggtag ttgatcacca cgtcggcgcc
     3301 gagctcacgg cagacgtcga gcttctcgtc ggagcccgcg gtcgcgatga ccttggcgcc
     3361 caaagccttg ccgatctgca ccgcgcccga gccggtgccg cccgccgccg cgtggacgag
     3421 caacgtctct tcgggctgca agcgcgcgcg ttcctcgagc gcaaaaagtc cgaggtggaa
     3481 ggggtagtgg agcgcggccg cgtctgccgc atccatctcg gcgggaatgt gcaacgtcgt
     3541 cgccgcgtcg accaatgcgt actcggcgta gccgccgtgc gcgaaaaccg ggatgccgac
     3601 gacgcgctga ccgagcaagt gctccgcgcc cggaccggcc gcctcgacga tgccgaccga
     3661 ctccatgccc ggtgtgaacg g
//
LOCUS       DASUGA010000125         4484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_327937, whole genome shotgun sequence.
ACCESSION   DASUGA010000125 DASUGA010000000
VERSION     DASUGA010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327937"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1946)
                     /locus_tag="VFX21_02400"
     CDS             complement(<1..1946)
                     /locus_tag="VFX21_02400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5094831.1"
                     /translation="MDGRTIRNAVGHALDAVLLVRAVDAATMVVEAANEAARALLGVE
                     TGQNLAEAGTSRLAALTPRLLLAAQRARATREPFVVRSETGAEVRVEVQLETMKGTDG
                     STRVMAVFRTGEIDLGREETGDDPTVGVFRVERGIGFVFVDDALCNLLGLSHEQALGQ
                     GWLDAVVPDDRSRVDHALARADGPTEAIDLECRVLRHGVDERNVRIRAVPVRGDDGAL
                     TGYLGSIEDDTEQRRSLRANTRLAELVDAVQEIVGVSDASWRMVYANPSCRQHLCIPD
                     DEPLGSFSAVDFIPEELREEMTQQIGRHLDRDGRWSGSLVLRAQDGHLLDVECTLLRH
                     GEVTDPDHYVSFLGRDVTALHQAEGARRESEERFRLMAEASAVGIFMIGRGGVVEYAN
                     PRIGEMLARPLPEVLGSIVTNSVHPDDKKRVYAESNAAARERRESTLEFDAVRPSGEI
                     RRLRANSVPVFDEAGAVRGFVGSVLDITDERRAESRLSAGAAWFRSLVQHAPDLLAVL
                     DADGVVRYVSPSAARLTGYEPEELIGRRLSELTEFDISGVAQQISENPGTPVGVVAPM
                     RHRDGTERMFEADITNLLDDQYVHGFVVNARDVTDRTEAEAGRRRSETALRAIVQASP
                     LAIFALDRAGTVHLWNAASERMFG"
     gene            complement(2101..2616)
                     /locus_tag="VFX21_02405"
     CDS             complement(2101..2616)
                     /locus_tag="VFX21_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094832.1"
                     /translation="MTEPDRPSDLERGRALMRDAEAAMRDGVDRLAAAWVVREVTRIV
                     DAWGRLDDGTRAQVLAGARMAGDDARARVLGELDALFALAPAAQRATPLQIVRSLRFE
                     VTAVLSAAGIPDVVRDEFDARAFPDDRYGVVPKAFADLGDDELGPMLMAWGVGKTLVL
                     RASTRDDSGDL"
     gene            complement(2613..2864)
                     /locus_tag="VFX21_02410"
     CDS             complement(2613..2864)
                     /locus_tag="VFX21_02410"
                     /inference="COORDINATES: protein motif:HMM:NF020940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HEX5094833.1"
                     /translation="MTHHDALRLFVSFPESLVGRPLLYEIIKEFDVVPNVRRANVEQS
                     SGWLILELAGTHDACDQAIEYLCAQGCTVNHREGDVVAG"
     gene            complement(2861..3637)
                     /locus_tag="VFX21_02415"
     CDS             complement(2861..3637)
                     /locus_tag="VFX21_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEX5094834.1"
                     /translation="MEPVQPSIGWGVLHLYYRVDRARAESEAEPAKRIVDAVAALEAD
                     GHQALCVAVLGHKADFGVMALGPDLARLHAFQQELLEAPLVPEYSFVSLTELSEYTST
                     EADERARLEREEQITGADADERVKVWAERMAHYQENRLHPRLPPKQLLCFYPMSKRRT
                     PGDNWYTLDFADRKRLMGGHARVGRRYAGRILQLITGATGIDDWEWGVTLLTDDAVAL
                     KDIVQEMRFDEVSARYADFGPFYTGLVLPPADACARVGLR"
     gene            complement(3653..>4484)
                     /locus_tag="VFX21_02420"
     CDS             complement(3653..>4484)
                     /locus_tag="VFX21_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094835.1"
                     /translation="DAQSARMAITIGFEGGGGGEYADFFASYEVSSEGMTDLQSGDSA
                     VTVDLGSAAAALATATGKDVEMVAIIETRNVGGVAYMRSPIFTEALDTGRSWISINAA
                     ALLGVDDETISQLSGQSNPLANITLLSGASRGVEEVGHEEVRGVETTHYTATVSLGEL
                     AEQELEALPPELRDKVGPGGPALAQFGRLGAYLHDIDAPTEVWVDDDGRLRKMTMLID
                     MRRALGSVAPPVEDLDDVDDLSLAYTFEMYDYGVDVDVEAPPADEVMDVTDGIDDLID
                     "
BASE COUNT          699 a         1594 c         1472 g          719 t
ORIGIN      
        1 cagccgaaca tgcgctcgga cgccgcattc cagaggtgca ccgtgcccgc gcggtcgagg
       61 gcgaagatcg cgagcggcga cgcctgcacg atcgcccgca acgcggtctc actgcggcgc
      121 cgtccggctt cggcttcggt gcggtcggtg acgtcgcgcg cgttgacgac gaacccgtgc
      181 acgtactggt cgtcgagcag gttcgtgatg tcggcttcga acatgcgctc ggtaccgtcg
      241 cgatgccgca tcggagcgac gacacccacg ggtgtgccgg ggttctcgct gatctgctgc
      301 gcgacaccgc tgatgtcgaa ctcggtgagc tcgctgagcc gccggccgat cagctcctcg
      361 ggctcgtagc cggtcaggcg ggccgccgac ggactgacgt agcgcaccac gccgtccgcg
      421 tcgagcaccg ccagcagatc gggggcgtgc tgcacgagcg accggaacca cgccgcgccg
      481 gccgacaagc ggctttcggc ccggcgctcg tcggtgatgt cgagcaccga accgacgaac
      541 ccgcgcacgg caccggcttc gtcgaacacg ggcaccgagt tcgcacgcag acgacggatc
      601 tcgccgctgg gacggacggc gtcgaactcg agcgtcgact cgcgccgttc tcgcgcggcc
      661 gcgttgctct cggcgtacac ccgcttcttg tcgtcgggat gcacgctgtt cgtgacgatg
      721 cttccgagca cctccggcaa cgggcgggcc agcatctcgc cgatacgcgg gttcgcgtac
      781 tcgacgacgc cgccgcgccc gatcatgaag atgccgacgg ccgacgcctc ggccatgagc
      841 cggaagcgct cttcgctctc gcgccgcgcc ccctccgctt ggtgcagggc cgtgacgtcg
      901 cgcccgagga acgagacgta gtggtcggga tccgtgacct cgccgtgtcg caagagcgtg
      961 cactcgacgt cgagcagatg accatcttgt gcgcgcagca ccagcgagcc cgaccagcgc
     1021 ccgtcgcggt cgaggtgccg accgatctgc tgcgtcatct cctcgcgcag ttcctcggga
     1081 atgaagtcga cggcggagaa cgaaccgagc ggttcgtcgt cggggatgca gaggtgctgc
     1141 cggcacgacg gattcgcgta gaccatccgc cagctcgcgt ccgagacccc gacgatctct
     1201 tgcacggcgt cgaccaactc ggcgaggcgc gtgttggcgc gcagcgaacg gcgctgctcc
     1261 gtgtcgtcct cgatcgatcc gaggtagccg gtcagcgcgc cgtcgtcgcc ccgcacggga
     1321 acggcacgaa tgcgaacatt gcgctcgtcg acgccgtgac ggagcacgcg gcactcgaga
     1381 tcgatcgctt cggtcggacc gtcggcgcgt gcgagcgcgt ggtccacgcg cgagcggtcg
     1441 tcgggaacga cggcgtcgag ccagccctga ccgagggcct gttcgtgact gagcccgagc
     1501 aggttgcaga gcgcgtcgtc gacgaagacg aagccgatgc cgcgttcgac gcgaaagacg
     1561 ccaacggtcg ggtcgtcgcc agtctcctcg cgaccgaggt cgatctctcc ggtgcggaac
     1621 acggccatga cgcgcgtgct gccgtcggtg cccttcatcg tttcgagctg cacttcgacg
     1681 cgcacttcgg cgcccgtctc ggagcgcacg acgaacggct cgcgcgtcgc gcgcgcccgt
     1741 tgcgcggcga ggagcaagcg cggcgtcagc gccgcgaggc gcgacgtgcc ggcctcggcg
     1801 aggttctggc cggtctcgac gccgagcagg gcgcgcgccg cctcgttcgc ggcttccacg
     1861 accatcgtcg ccgcgtcgac ggcacgaacg agcagtaccg cgtcgagcgc gtgcccgaca
     1921 gcgttccgta tcgttcgccc gtccaccgcc ggtccgtggt cagggccttg cccggtcatc
     1981 ctgttccccc cgatgcccgc cgggcgccat ggtggcctgt ccacacgccg tccacaaggt
     2041 gtgacggctt gcccacagct tgtccacaac tctggtcgcg cactgcggct gcacagaggt
     2101 tcacaaatcc cccgaatcat cgcgcgtgct cgcgcgcagc acgagcgtct tgcccacgcc
     2161 ccacgccatc aacatcggcc ccagctcgtc gtcgcccaag tcggcgaacg ccttcggtac
     2221 gaccccgtac cgatcgtcgg ggaacgcgcg tgcgtcgaat tcgtcgcgca cgacgtccgg
     2281 aatgcccgcc gccgacaaca ccgcggtcac ctcgaagcgc aagctgcgca cgatctgcaa
     2341 cggtgtcgcg cgttgtgctg cgggcgcgag cgcgaagagc gcatcgagct cgcccagcac
     2401 gcgcgctcgc gcgtcgtcac ccgccatccg cgcgccggcc agcacctgcg cgcgagtccc
     2461 gtcgtcgaga cgaccccacg cgtcgacgat gcgcgtcacc tctcgtacga cccatgccgc
     2521 agccaaccgg tcgacaccat cgcgcatcgc agcttccgcg tcgcgcatga gcgcgcgccc
     2581 acgctcgaga tcagacgggc ggtcgggttc cgtcaacccg cgactacatc gccttccctg
     2641 tgattcacgg tgcagccctg cgcgcacaag tactcgatcg cctggtcgca cgcgtcgtgg
     2701 gtgcccgcga gctcgaggat cagccatccc gacgactgct cgacgttggc gcgccggacg
     2761 ttgggaacga cgtcgaattc cttgatgatc tcgtacagga gcgggcgccc gacgagcgac
     2821 tcggggaacg acacgaacag ccgcagcgcg tcgtgatgtg tcatcgcaat cccacccggg
     2881 cgcacgcatc ggcgggcggc agcaccagcc cggtgtagaa ggggccgaag tcggcgtagc
     2941 gcgccgacac ttcgtcgaag cgcatctcct gcacgatgtc cttcagggcg acggcatcgt
     3001 cggtgagcaa cgtgacaccc cactcccagt cgtcgatgcc ggtcgcgccc gtgatcaact
     3061 gcaggatgcg accggcgtac cggcgaccga cgcgcgcgtg gccgcccatc aaccgcttgc
     3121 ggtccgcgaa gtcgagtgtg taccagttgt cgcccggcgt gcgtcgtttc gacatcggat
     3181 agaagcagag cagctgcttc ggcggcagcc ggggatgcaa ccggttctcc tggtagtgcg
     3241 ccatgcgttc ggcccagacc ttgacgcgct cgtccgcgtc ggcgccggtg atctgctctt
     3301 cacgctcgag ccgcgcgcgt tcgtcggctt cggtcgatgt gtactcggag agctcggtga
     3361 gcgagacgaa cgagtactcg ggcaccagcg gtgcctcgag cagctcctgt tggaacgcgt
     3421 gcaggcgggc gaggtcaggg cccagtgcca tgacaccgaa gtcggccttg tgcccgagca
     3481 cggcgacgca caacgcctgg tgcccgtcgg cctcgagcgc ggcaacggcg tcgacgatcc
     3541 gcttggccgg ctccgcttcg ctctccgccc gcgcccggtc gacgcggtaa tagaggtgca
     3601 ggactcccca cccgatcgac ggctgcaccg gctccatacc cggccaggct actcagtcga
     3661 tgaggtcgtc gatcccgtcc gtgacgtcca tgacctcgtc cgcgggcggt gcttcgacgt
     3721 cgacatcgac gccgtagtcg tacatctcaa acgtataggc gaggctgaga tcatcgacgt
     3781 cgtcgaggtc ttcgaccggc ggtgccaccg acccgagggc gcgacgcata tcgatcagca
     3841 tcgtcatctt ccgcaaccgg ccatcgtcgt ccacccacac ttccgtgggc gcgtcgatgt
     3901 cgtggaggta cgcgccgagt cgaccgaact gggccagcgc ggggccacct ggaccgactt
     3961 tgtcgcgcag ctccggcggg agcgcctcga gttcctgttc tgccagctca ccaaggctca
     4021 ccgtggcggt gtagtgcgtc gtctcgaccc cgcggacctc ttcgtgtccg acttcttcga
     4081 ctcctcggga cgcgccgctc aagagcgtga tattggcgag gggattcgac tgaccggaaa
     4141 gctgcgagat cgtttcatca tcgacaccga gcagagctgc ggcgttgatc gagatccagg
     4201 acctgcccgt atcgagtgct tccgtgaaga tcggcgatct catgtacgcg acgccaccaa
     4261 cattgcgcgt ctcgatgatg gcgaccatct cgacgtcttt gcccgtcgct gtggccagcg
     4321 cagcggccgc gctgcccagg tcgaccgtga cggcgctatc acccgattgc aggtcggtca
     4381 tgccctccga gctgacctcg tacgacgcga agaagtccgc gtactcgccg ccaccaccgc
     4441 cttcgaagcc gatggtaatc gccatgcgcg cggattgcgc gtcg
//
LOCUS       DASUGA010000126         4569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1994710, whole genome shotgun sequence.
ACCESSION   DASUGA010000126 DASUGA010000000
VERSION     DASUGA010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1994710"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="VFX21_02425"
     CDS             complement(<1..1022)
                     /locus_tag="VFX21_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094836.1"
                     /translation="MAGSVELGREAFARAAWADAHAHLSASELAGPDDHELYAVASYM
                     SGHDDDSAAAWERAHTLALGADDVEQAARCGAWLAIALLLKGEHAHASGWLARVERVL
                     ANTDADVPARGILLVPAFLGALDGGDLTAADAMATEMVAIGSRLDDADVLAFGLLCHG
                     EVLAAAGDVAGGMRRLDEAMVSVTAGETGPITSGIIYCAVIEACMAVSDMRRAGEWTA
                     ALTAWCASQPGLVPYRGQCLVHRSEILLARGTWTEALAEATRARDRLSDPVHPALGDA
                     YYQQGELHRLLGSYDEAEQAYRAAAAHGREPAPGFALLRLAAGDVGAARAAITRVAAE
                     SGGVIPR"
     gene            1112..2374
                     /locus_tag="VFX21_02430"
     CDS             1112..2374
                     /locus_tag="VFX21_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HEX5094837.1"
                     /translation="MAGVLHSPLSIGAMTVRNRLYRAPVLEGAGDGDDAAERYARHFV
                     ENARHGVGMVVQGSSCVFAEGRTSPGMTCVDTREKTLRLAPMVEAVHREGAAIVLQIG
                     HGGLFAMEAWHEPYASRRTGPILTSSPVPKAMWPVFRGVPVHPMTTDEVYAMARQYGA
                     VASWAREAGYDAVQLGSANAKLLDQFLSPFYNRRTDEFGGSLEGRARVLRIIRDEIRT
                     QAGADYPCLVKVPAETAPLWFARSTFDDAIDLARLVEEWGFDAVTPVRVSVLPDTTLS
                     RGGTPDSFTTNKAMAQRLRTAAPSRWRRFVLVYGAKVGARRARFEPVWNRELFTAVRA
                     NVSIPVLAVGGIRTAAECNEILDAGVADMVGIGRPFYAEPDLAERILGDDTAPRLCRN
                     SNRCVPAQMLGMKGMCYNPAVRTRSRDS"
     gene            2384..2767
                     /locus_tag="VFX21_02435"
     CDS             2384..2767
                     /locus_tag="VFX21_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094838.1"
                     /translation="MAITGVANLAVKVRDLDEAVAFYRRAGATVTDPEAWRDGRRADV
                     HLGPLQITLFTRAIYEDDVALPDEGFLHVALFSDSVDREIEGHDVVWGPAVVQGPTFG
                     TRRIVFVDAPGGMRLEFMEQLEDPQ"
     gene            2764..3135
                     /locus_tag="VFX21_02440"
     CDS             2764..3135
                     /locus_tag="VFX21_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5094839.1"
                     /translation="MKVTRVLHVSVNVEGALDETQSFYTQLFGLDSKTRPEIPGVAGH
                     WFALGDTELHLVDADEGTTRIRSRGNHYCVGVDDIDAAIAELEARGIEYQRGAQGDVV
                     QIWVVDPAGNTIELQQDRTHP"
     gene            3132..4208
                     /locus_tag="VFX21_02445"
     CDS             3132..4208
                     /locus_tag="VFX21_02445"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5094840.1"
                     /translation="MTLPLGRPARVGVVGLGRIYDLVARGYRSNDDLEIVALCDPDPA
                     RLAERGAEWPDAQQFTDLDDLLGADLDAVDVLVPTPLHCEVVCKVLRAGLHVNMQKPF
                     ANTLAEADEMIATADAAGVYLRVMENFVYYEPLRELKRVVESGEIGEPAGLHMKMVAT
                     GNGGWEVPWSAWEWQFKQARSGRGILAFDDGWHKFSVAHWLFGPVVEIMAWIGRTEVV
                     PDLVVIDAPTTIMWTHANGLRGVFDITFAPDMLMKSNYYTNDERFEVTGRKGFARVNR
                     CTSLGIQQPSLEVYHDGELRASHALDDDWGSSFDRQTRDFYRYLVHGGEEPLWTAQQA
                     REVLALNLAAYDSAAANGPVQLSN"
     gene            complement(4215..>4569)
                     /locus_tag="VFX21_02450"
     CDS             complement(4215..>4569)
                     /locus_tag="VFX21_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013805794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5094841.1"
                     /translation="VFANATNDMRIAREEIFGPVLAVIPYDDEADALRIANDSEYGLA
                     GSVWTGDAERGMEIARRVRTGTYGINQYTMDFVAPFGGYKASGIGREFGREGLEHYVE
                     SKSIVPPGGGVAAAG"
BASE COUNT          723 a         1534 c         1606 g          706 t
ORIGIN      
        1 cgagggatca cgccgccgct ctcggccgcg acgcgtgtga tcgcggcgcg cgcggcaccg
       61 acgtctcccg cggccaggcg caacagtgcg aacccgggcg ccggttcgcg accatgcgcg
      121 gccgccgccc ggtatgcctg ctcggcttcg tcgtacgaac cgagaagacg gtgcaattcg
      181 ccctgttggt agtacgcgtc gcccaacgcg ggatgcaccg ggtcggagag gcggtcgcgg
      241 gctcgggtgg cctccgcgag cgcttcggtc cacgtcccgc gcgccaacaa gatctcggat
      301 cgatgcacga ggcactgacc gcggtacgga accaggccgg gttgcgacgc gcaccaggcc
      361 gtcagggcgg cggtccattc accggcgcgg cgcatgtcgc tgaccgccat gcacgcctcg
      421 atgacggcgc agtagatgat gccggaagtg atcggaccgg tctcgccggc cgtcaccgag
      481 accatcgctt cgtcgagtcg cctcatgccg cccgccacat cacccgcggc cgccagcacc
      541 tcgccgtggc acaacaggcc gaaggcaagg acgtcggcat cgtcgagacg agatccgatc
      601 gcgaccatct cggtggccat tgcgtccgca gccgtcagat cgccgccgtc cagcgcgccg
      661 aggaacgcgg gaacgagcag gatcccacgg gcggggacat ccgcgtctgt gttcgcgagc
      721 acgcgctcga cccgcgccag ccatccactc gcgtgcgcgt gttcaccctt caacaacaaa
      781 gcgatcgcca accacgcacc gcaacgagcg gcctgctcga cgtcgtcggc accaagggcg
      841 agcgtgtggg cgcgttccca cgcggccgcg ctgtcgtcgt cgtgcccgga catataggac
      901 gcgacggcgt agagctcgtg gtcgtcgggg ccggcgagct ccgacgcgga caggtgcgcg
      961 tgcgcgtcgg cccacgccgc gcgggcgaac gcttcccgac cgagttcgac cgaaccagcc
     1021 accgccacca gtgtcgcgga ccttcggtcg cagggcgagg ggtcgcgtcg cactggacgg
     1081 gcccgggggt gcaggcggta gacatcgagt gatggccggt gtcctgcact cgccgctctc
     1141 gatcggtgcg atgacggtcc gcaaccgtct ctaccgcgcg cccgtgctcg agggggccgg
     1201 agatggcgac gacgccgcgg agcgttatgc acggcacttc gtcgaaaacg cgcgccacgg
     1261 ggtcggcatg gtcgtccagg gctcgtcgtg cgtgttcgca gaagggcgca cgtcgcccgg
     1321 catgacatgt gtcgacacgc gtgagaagac gttgcggctc gcgccgatgg tcgaggccgt
     1381 gcatcgtgaa ggcgcggcga tcgtgctcca gatcgggcac ggcgggctgt tcgcaatgga
     1441 ggcgtggcac gagccgtacg cttcgcggcg caccggaccg atcctgacgt cgtcgccggt
     1501 gccgaaggcg atgtggcccg tgttccgcgg tgtgccggtg cacccgatga cgaccgacga
     1561 ggtctacgcg atggcgcgcc aatacggcgc ggtcgcgagt tgggcgcgag aagccgggta
     1621 cgacgcggtg cagctcggtt cggcgaacgc gaagttgctc gaccagttcc tctcgccgtt
     1681 ctacaaccgt cgaaccgacg agttcggcgg ctcgctcgag ggacgcgcgc gtgtgctgcg
     1741 gatcatccgc gacgagatcc gcacgcaggc cggcgccgac tatccgtgtc tcgtgaaggt
     1801 gccggcggag accgcgccgt tgtggttcgc gcgctcgacg ttcgacgatg cgatcgacct
     1861 ggcgcggttg gtcgaggagt ggggattcga cgcggtgacg ccggtgcgtg tgtcggtgct
     1921 cccggacacg acgctctcgc gtggcggcac gcccgactcg ttcacgacga acaaggccat
     1981 ggcgcagcga ttgcggacgg ccgcgccgtc ccgctggcgg cggttcgtct tggtgtacgg
     2041 cgcaaaggtc ggcgcgcggc gggcgcgttt cgaaccggtg tggaaccgcg agctgttcac
     2101 ggcggtccga gcgaacgtgt cgatcccggt gcttgcggtg ggcggcatcc gtaccgcggc
     2161 cgagtgcaac gagatcctcg acgccggtgt cgccgacatg gtcggcatcg gccgcccctt
     2221 ctacgccgag cccgatctgg ccgaacgcat cctcggcgac gacaccgcgc cgcgcctgtg
     2281 ccggaactcg aaccgctgcg tccccgcgca gatgctcggc atgaaaggca tgtgttacaa
     2341 ccccgcagtg cgcacccgga gccgagactc gtaaggtcgg cgcgtggcga tcactggtgt
     2401 tgcaaatctg gcggtcaagg tgcgcgatct cgacgaagcg gtcgcgttct accgccgggc
     2461 cggggccacg gtgaccgatc ccgaagcgtg gcgcgacggc cggcgggcag acgtgcacct
     2521 cgggccgctg cagatcacgt tgttcacgcg cgcgatctac gaagacgacg tcgcgctgcc
     2581 cgacgagggc tttctgcacg tcgcgttgtt cagcgactcc gtcgatcgcg agatcgaagg
     2641 ccacgatgtg gtctgggggc cggcggtcgt gcagggcccc acgtttggca cgcgccgcat
     2701 cgtgttcgtc gatgcaccgg gcgggatgcg gttggagttc atggagcagc tggaggatcc
     2761 gcagtgaaag tcacccgcgt cttgcacgtc agcgtgaacg tcgagggtgc actcgacgag
     2821 acgcagtcgt tctacacgca gctcttcggg ctcgactcga agacgcggcc cgagatcccc
     2881 ggcgtcgccg gccactggtt cgcgctcggc gacactgaac tgcacctcgt cgacgccgac
     2941 gaagggacga cacggatcag gtcgagaggc aaccactact gcgtcggcgt cgacgacatc
     3001 gatgccgcca tcgccgagct cgaggcgcgc ggcatcgagt accagcgggg cgcacagggt
     3061 gacgtggtgc agatctgggt cgtcgacccc gccggcaaca cgatcgaact gcaacaggat
     3121 cggacgcacc catgaccctc ccgctcggac gacccgcacg cgtcggtgtc gtcggcctcg
     3181 gccgcatcta cgacctcgtc gcgcgcgggt atcgcagcaa cgacgacctc gagatcgtcg
     3241 ccctctgcga tcccgacccg gctcgtctcg ccgagcgcgg tgccgagtgg ccggacgcgc
     3301 agcagttcac cgatctcgac gacctactcg gcgcggacct cgacgccgtc gacgtcctcg
     3361 tgccgacgcc gctgcactgc gaggtcgtgt gcaaagtgct gcgcgccggg ctgcacgtga
     3421 acatgcagaa gccgttcgcg aacacgctcg cggaagccga cgagatgatc gcaacggccg
     3481 atgcggccgg cgtgtacctg cgcgtgatgg agaacttcgt ctactacgaa ccgttgcgcg
     3541 agctgaagcg tgtcgtcgag tcgggcgaga tcggcgaacc ggccgggctg cacatgaaga
     3601 tggtcgcgac gggcaacggc ggctgggaag tgccctggtc ggcgtgggag tggcagttca
     3661 agcaagcgcg cagtgggcgc ggcatcctgg cgttcgacga cgggtggcac aagttctcgg
     3721 tcgcgcactg gttgttcgga ccggtggtcg agatcatggc gtggatcggc cgcaccgagg
     3781 tcgtgcccga cctggtcgtg atcgacgcgc cgaccacgat catgtggacg cacgccaacg
     3841 ggctgcgcgg cgtcttcgac atcacgttcg cgcccgacat gttgatgaag tcgaactact
     3901 acaccaacga cgaacgcttc gaggtgacgg gccgcaaggg cttcgcgcgc gtcaaccggt
     3961 gcacgtcgct cggcatccag caaccgtcgc tcgaggtgta ccacgacggc gaactgcggg
     4021 cgtcgcacgc gctcgacgac gactggggtt cgtcgttcga ccgccagacg cgcgacttct
     4081 accgttacct cgtccacggc ggcgaggagc cgctctggac tgcacagcag gcgcgcgagg
     4141 tccttgcgtt gaacctcgcg gcctacgact cggccgcggc caatgggccg gtgcagttgt
     4201 cgaactgagc gcgctcagcc ggcggcggcc acgcccccgc ccggcggcac gatcgacttg
     4261 ctctcgacgt agtgctcgag gccttcacga ccgaactcgc ggccgatgcc cgatgccttg
     4321 tagccaccga acggtgcgac gaagtccatc gtgtactggt tgatgccgta ggtgccggtg
     4381 cgtacgcggc gcgcgatctc catgccgcgt tcggcgtcac cggtccacac cgaaccggcc
     4441 aggccgtatt cgctgtcgtt ggcgatgcgc aacgcgtcgg cttcgtcgtc gtaaggaatc
     4501 acggcgagca ccgggccgaa gatctcttcg cgagcgatgc gcatgtcgtt cgtcgcgttg
     4561 gcgaacacg
//
LOCUS       DASUGA010000127         3914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_206458, whole genome shotgun sequence.
ACCESSION   DASUGA010000127 DASUGA010000000
VERSION     DASUGA010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206458"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VFX21_02455"
     CDS             complement(<1..387)
                     /locus_tag="VFX21_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755827.1"
                     /note="with PdxT forms pyridoxal 5'-phosphate from
                     glutamine, either ribose 5-phosphate or ribulose
                     5-phosphate, and either glyceraldehyde 3-phosphate or
                     dihydroxyacetone phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX5094842.1"
                     /translation="MPEDRTTGTARVKRGLAEMLRGGVIMDVVTPEQAKIAEDAGASA
                     VMALERVPSDIRRDGGVSRMSDPSMIEGIQKVVTIPVMAKCRIGHFAEAQVLQALGVD
                     YIDESEVLTPADEAHHVDKWAFTTPFV"
     gene            complement(408..1418)
                     /locus_tag="VFX21_02460"
     CDS             complement(408..1418)
                     /locus_tag="VFX21_02460"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HEX5094843.1"
                     /translation="MRRLILAICAGALALSACSSGGGDTKSSAAAATTTTKPPPPIAP
                     LTGLPDPSGAARNRCAVTVKIDNTQAGHPKYNVDQADVIYEEVVEGGITRLAAIFNSH
                     NPDRVGPVRSVRKTDHLLVAPLKGVFAYSGGAPDSVASINTAPVVLLDENRAGNAMFR
                     DGNRRAPWNLYARVDQMYGRCKDPAPPALFTYRDPKTPVTAGTPLTSALVNFRAGFEV
                     RWTWDAPSGTWKRRIFGGPEMTASNLQFAPQNVVIMYAPYAGGDARPQYFMYGAEAQL
                     VGTGRLQVLTGGKLIDGTWSRPAANQRARLLDKAGSEIRLAPGQTWVEIPEPTYSVTV
                     TP"
     gene            complement(1435..2346)
                     /gene="htpX"
                     /locus_tag="VFX21_02465"
     CDS             complement(1435..2346)
                     /gene="htpX"
                     /locus_tag="VFX21_02465"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX5094844.1"
                     /translation="MYEQIAANKRKTFLIFAGFIILLLAVGWTINYLIQGGVVFGAVI
                     AIFVIGSSIFSYYKSDAIALRVSRAVPADPTQYARYYNLVEGLTIASGLPMPRLYIVN
                     DDAPNAFATGRNPKHAAVAVTTGLLEKMNRVELEGVLAHELSHIKNYDILIMTISVTL
                     VGVVVLLSDVLLRAMFWGGGRRDGDGNGNGGSPVAAILAIFGFLLIILSPLIARLMQF
                     AVSRKREYLADASGVQLTRYPPGLISALKKLEDDKTSVQTSSKATAHLWIEEPLDKES
                     NRGQNKFNRLFDTHPPIGDRIHALEQM"
     gene            complement(2353..2913)
                     /locus_tag="VFX21_02470"
     CDS             complement(2353..2913)
                     /locus_tag="VFX21_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX5094845.1"
                     /translation="MIAVIIIVAIVVIAALVFVGMYNGLVKLRNRVDSAWSQISVQLQ
                     RRHDLIPNLVETVKGYAAHERQTLEAVTSARNAAVQAQQGAGPAQQAQAENVLTGTLR
                     QLFALSEAYPDLKANQNFLNLQEELTATEDRVSYARQFYNDQVQKLNTKIQSFPTNII
                     AGMFNFDEREFFAAEEGATSVPKVQF"
     gene            complement(2947..3861)
                     /locus_tag="VFX21_02475"
     CDS             complement(2947..3861)
                     /locus_tag="VFX21_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX5094846.1"
                     /translation="MSTDPKVETLAIDIAAAIRDAVAPSLGDPAARERVGIAPGGDVT
                     MAIDDIAEHVLHRMCTDAGDIAFYSEDQGYVEFGTPRAIFVVDPIDGTRPAAAGLESC
                     CVSVGVVPPSLDATLGDVELGIVHEIKSGRAFVARRGEGYPGALSSNTDLGALFWTAG
                     LRCRPALETTMVLQDLIDGCSMRGGYFDLGSCTFNMTRIVTGQLDAYVDLGQHLVDTI
                     PGLVEKFRAIADGAVGVNWPYDVAAATLILQEAGGVVTRPDGGSIADHPAVGSGDGFG
                     LAIIASASPALHELLVGAVATGMTRLAP"
BASE COUNT          649 a         1269 c         1314 g          682 t
ORIGIN      
        1 gacgaaggga gtggtgaacg cccacttgtc gacgtggtgg gcttcgtcgg ccggggtcag
       61 cacttccgat tcgtcgatgt agtcgacgcc gagcgcttgc agcacctgcg cttccgcgaa
      121 gtgaccgatg cggcactttg ccatcacggg aatcgtgacg accttctgaa taccctcgat
      181 catcgacggg tcgctcatgc gtgacacgcc accgtcgcgg cggatgtcgg acggcacgcg
      241 ttcgagggcc atgacggccg atgcgcccgc gtcttcggcg atcttcgcct gctcgggcgt
      301 gacgacgtcc atgatgacgc cgccccgcaa catctcggcc aggccgcgct tgacgcgcgc
      361 cgttccggtg gtgcgatctt cgggcatagc cggagtttac gcagccacta gggcgtgacc
      421 gtcaccgaat acgtgggttc gggaatttcg acccaggtct gacccggcgc caaccggatc
      481 tcgctgccgg ccttgtcgag gagccgggcc cgctggttgg ccgcaggccg cgaccacgtg
      541 ccgtcgatga gtttgccgcc ggtcagcact tgcaaccggc cggtgccgac gagctgcgct
      601 tcggcgccgt acatgaagta ctgcggccgc gcatcaccac cggcgtacgg cgcgtacatg
      661 atcaccacgt tctgcggcgc gaactggaga ttgctcgcag tcatttccgg tccgccgaaa
      721 atgcgccgct tccaggtgcc gctcggcgca tcccacgtcc agcgcacttc gaacccggcg
      781 cggaagttca cgagcgccga cgtgagcggc gtccccgcag tcaccggcgt cttcgggtcg
      841 cgataggtga acaacgcagg cggcgccgga tccttgcatc gcccgtacat ctggtcgacg
      901 cgagcgtaga gattccacgg tgcgcgccgg ttgccgtcgc ggaacatcgc gttgccggcg
      961 cggttctcgt cgaggagcac cactggtgcg gtgttgatcg acgccacgga atccggtgca
     1021 ccacccgagt acgcgaacac acccttcaac ggcgcgacga gcaggtggtc ggtcttacgc
     1081 accgagcgca ccgggccgac gcggtccggg ttgtgcgagt tgaagatggc ggcaaggcgc
     1141 gtgatgccgc cctcgacgac ttcttcgtag atcacgtcgg cctggtcgac gttgtacttc
     1201 gggtgtcccg cctgcgtgtt atcgatcttg acggtgaccg cgcaacggtt gcgggctgcg
     1261 ccggagggat cgggcaaacc cgtgagcggc gcgatcggcg gcggcggctt cgtggtcgtg
     1321 gtcgcagccg cggccgagga cttcgtgtcg ccaccgccgc tgctgcacgc gctcaatgcg
     1381 agcgcgcccg cgcatatcgc caggatcagc cgccgcatcg tcaaaagtct gtccctacat
     1441 ctgctcgagg gcgtggatgc gatccccgat cggcgggtgc gtgtcgaaca ggcgattgaa
     1501 cttgttctgg ccgcggttcg actccttgtc gagcggctcc tcgatccaca agtgcgcggt
     1561 cgccttcgac gacgtctgca ccgaggtctt gtcgtcctcg agcttcttga gcgccgagat
     1621 cagcccgggc gggtaccgcg tgagttgcac ccccgacgcg tcggcgaggt actcccgttt
     1681 ccggctcacc gcgaactgca tgagccgggc gatgagcggc gagaggatga tgagcaggaa
     1741 gccgaagatc gccaggatcg cggccaccgg gctgccgccg ttgccgttgc cgtcgccgtc
     1801 gcgccggcca ccgccccaga acatcgcgcg cagcaacacg tcggagagca gcacgacgac
     1861 accgaccagc gtgaccgaga tcgtcatgat caagatgtcg tagttcttga tgtggctgag
     1921 ctcgtgcgcg agcacgccct cgagttcgac gcggttcatc ttctcgagca ggccggtcgt
     1981 gaccgcgacc gccgcgtgct tggggttgcg tccggtcgcg aacgcgttcg gtgcgtcgtc
     2041 gttgacgatg tagagccggg gcatcgggag gccgctcgcg atggtcagcc cttcaaccag
     2101 gttgtaatac cgcgcgtact gcgtcggatc ggcgggcacc gcgcgcgaaa cccgcaacgc
     2161 gatcgcgtcg ctcttgtagt acgagaagat cgacgagccg atgacgaaga tcgcgatgac
     2221 cgcgccgaag acgacgccgc cttggatgag atagttgatg gtccagccga ccgccagcaa
     2281 caagatgatg aagccggcga agatcagaaa tgtcttgcgt ttgttcgcag cgatttgttc
     2341 gtacacgggg agttagaact gcaccttcgg aactgaggtg gcgccttcct cggcggcgaa
     2401 gaactcgcgc tcgtcgaagt tgaacatgcc cgcaatgatg ttcgtcggga agctttggat
     2461 cttcgtgttc agcttctgca cttggtcgtt gtagaactgc cgggcatacg acacgcggtc
     2521 ttcggtcgcg gtcagctctt cctgcaagtt caagaagttc tggttggcct tgaggtcggg
     2581 gtacgcctcc gacaacgcga acagttggcg caacgtgccg gtcaggacgt tctcggcttg
     2641 ggcctgttgc gccgggccgg caccctgctg ggcctgcacc gcggcgttgc gagcggacgt
     2701 caccgcttcg agcgtctgcc gctcgtgggc ggcgtagccc ttcacggttt cgaccagatt
     2761 ggggatgagg tcgtggcggc gttgcaactg cacgctgatc tgcgaccacg ccgagtcgac
     2821 gcgattgcgg agcttgacga ggccgttgta catgcccacg aagacgaggg ccgcgatgac
     2881 gacgatcgcc acgatgatga tgactgcgat caagggatga cctcccgaag agggaaccgg
     2941 gtcattctac ggagcgaggc gtgtcattcc tgtcgcaacc gcgcccacca ggagttcgtg
     3001 caacgccggc gaggcgctgg cgatgatcgc gaggccgaat ccgtcgccgg agccgaccgc
     3061 cggatggtcg gcaatcgacc cgccatcggg gcgggtgacg acgccgccgg cctcttgcag
     3121 gatcaaggtc gcggccgcga cgtcgtaggg ccagttcacg ccgaccgcgc cgtcggcgat
     3181 cgcccggaac ttctcgacga ggcccggaat ggtgtcgacg agatgctggc cgagatccac
     3241 gtaggcgtcg agctggccgg tgacgatgcg cgtcatgttg aacgtgcacg agcccaggtc
     3301 gaagtagccg ccgcgcatgc tgcagccgtc gatgaggtct tggagcacca tcgtcgtctc
     3361 caacgccggc cggcaacgca accccgcggt ccagaacagc gcgccgaggt ccgtgttgga
     3421 cgacagcgcc cccggatacc cctcgccgcg gcgcgcgacg aacgcccggc ccgacttgat
     3481 ctcgtgcacg atcccgagtt cgacgtcgcc gagcgtcgcg tcgagcgacg gcggaacaac
     3541 cccgaccgac acacaacacg attccagtcc ggccgccgcc gggcgcgtgc cgtcgatcgg
     3601 gtcgacgacg aagatcgcgc ggggcgtgcc gaactccacg tagccctgat cctcggaata
     3661 gaaggcgatg tcgcccgcgt ccgtgcacat ccggtgcagc acgtgctcgg cgatgtcgtc
     3721 gatcgccatc gtcacgtcgc cgcccggcgc gatcccgacc cgttcgcgcg ccgccggatc
     3781 gccgagcgac ggcgcgaccg catcgcgaat cgctgctgcg atatcgatgg cgagcgtctc
     3841 aaccttggga tcggtactca tgcggcgttg acgagggcgc gttcgtaaag ctcgaggtag
     3901 cgctctgcaa gacc
//
LOCUS       DASUGA010000128         3659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1994214, whole genome shotgun sequence.
ACCESSION   DASUGA010000128 DASUGA010000000
VERSION     DASUGA010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1994214"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1042)
                     /locus_tag="VFX21_02480"
     CDS             complement(158..1042)
                     /locus_tag="VFX21_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEX5094847.1"
                     /translation="MIHIVEEVRDALAEGRGVVALESTIFSPFGLPAPANLDALTQCL
                     AVVRDAGAVPAVTAVLDGHARVGLDPAEHERILHGGRKVAARDLPVAIARDTPVGVTT
                     VSASLALAHAAGISMFATGGIGGVHRGEHATGDISSDLTALASHPVVTVCAGAKAFLD
                     LPRTLEYLETASVPVVGWQTDEFPMFWCRSSGLALSSRVDDAEQAARVFRAQRELTPT
                     HGVLICVPIPPEAAISPEIVQAAIDGVEEGATGAAVTPRVLAAIADATGGRTVAANIV
                     LAAHNASVAARIAEALVG"
     gene            complement(1039..1887)
                     /locus_tag="VFX21_02485"
     CDS             complement(1039..1887)
                     /locus_tag="VFX21_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX5094848.1"
                     /translation="MEPLVCTVGDLVRDVVVHTAQPMVRHADVPAHITAHRGGSAANT
                     AAAVVHAGGRARFVGCVGVDDDGMHLLTELANAGVDCVGARRGRTGVVVVVVEPDGER
                     TMFSDRGDSSALDLFEPAWLDDVAVLHVPAYAFVTQPLARTAESMLAAARERGILCSV
                     DPSSVTMIDDRLRDLLVRVQPDVLLCNREEAAALGVDDDGWGTVRYVVVKQGPAPVLL
                     RGAVSADIPTLVLDGPIDATGAGDAFAAGFLLSLARGASPLDAARNGHEIASKVVRGA
                     GADAWV"
     gene            1916..2551
                     /gene="upp"
                     /locus_tag="VFX21_02490"
     CDS             1916..2551
                     /gene="upp"
                     /locus_tag="VFX21_02490"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009883966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEX5094849.1"
                     /translation="MPLTVVDHPLVADRILTLRDERTKSVDFRRIAREVASFLAYEST
                     RDLPTEPDEVTTPLGLVARGVRPAVPGPIVVPILRAGLGLLDGVLSALPDAEVGVIGL
                     KRDETTHRPLAYLERLPDDLSGRHAYIVDPMLATGGSLVTAVEMLRSRGAGPIVCLCL
                     IAAPEGVELLRKADPDVHVFTAALDDKLNEHAFIVPGLGDAGDRLYGVGSA"
     gene            2570..3646
                     /locus_tag="VFX21_02495"
     CDS             2570..3646
                     /locus_tag="VFX21_02495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX5094850.1"
                     /translation="MARLPRYRTGDAEIDEQIADLIAKLGDDVRDTDLLFELVVSTVR
                     LARDHASRGDLKMANAALKEMRYAFAVFEPYRSARKAAIFGSARTRRDDPLYEQTVRL
                     AEALAAADWMVITGAGPGIMEAGIEGAGAASSFGVGIRLPFEAATSQFLADDPKLVNF
                     RYFFTRKVTFVKESHAFILLPGGFGTLDEAFELLTLVQTGKSQPAPIVLLDVPGGTYW
                     LTWLEFVHRELRDRGYISAEDLDLVKITDDVDVALAEVTGFYRNFHSLRFVAGDLVLR
                     IHEQPTEPELAALNDEFADIVSRGRIEIAEASPAEIADRDYPELPRLRFRFDRHGFAR
                     LRKLIDRLNAPEGLDGLRDRPAGL"
BASE COUNT          562 a         1316 c         1230 g          551 t
ORIGIN      
        1 gcggccagtg cgagcgctgc caccgcgccg agcgcgacga acctgtgaaa acgtcggatc
       61 ctcacccaat ctcctccccg cgcctgcagg cgcacccagc agatttttgg agcctaccga
      121 ttcgatgcgc gtacgtctgg gaccgaacgg ccttgcgtta tccgaccaaa gcctctgcaa
      181 ttcgcgccgc gacggacgcg ttgtgcgcgg cgagcacgat gttggcggcg accgtgcgcc
      241 cgccggtcgc gtccgcgatc gcggcgagca cgcgcggtgt gaccgcggca cccgttgcgc
      301 cctcttcgac gccgtcgatc gcggcttgga cgatttcggg ggaaatagcc gcttcaggag
      361 ggattgggac acaaatcaga acaccatgtg tcggagtaag ctcgcgctgc gcgcgaaaca
      421 cgcgggccgc ctgctcggca tcgtcgacgc gcgacgagag cgcgagcccg ctcgatcggc
      481 accagaacat cgggaattcg tcggtctgcc agccgacgac cggcacgctc gcggtttcga
      541 ggtactcgag cgtgcgtggc agatcgagga aggccttcgc gcccgcgcac acggtgacga
      601 ccgggtgcga cgcgagcgcg gtcagatcgg aactgatgtc gccggtcgcg tgctcgccgc
      661 ggtgcacgcc gccgatgccg ccggtggcga acatgctgat gcccgccgcg tgcgcgagcg
      721 cgagcgacgc cgagacggtg gtaacgccga ccggagtgtc ccgtgcgatc gcgacgggca
      781 ggtcccgcgc cgcgactttt cgcccgccgt gcaggatgcg ctcgtgctcc gcaggatcga
      841 gcccgacgcg agcgtgcccg tcgagcaccg ccgtgacggc gggcaccgcg cccgcgtccc
      901 gaaccaccgc gagacactgc gtgagcgcgt cgagattcgc cggcgcgggc aacccgaacg
      961 gcgagaagat cgtcgactcc agcgcgacga cgccgcgccc ttccgcgagc gcatcgcgca
     1021 cttcttccac gatgtggatc acacccacgc gtcggctcct gcgccgcgga cgactttgct
     1081 tgcgatctcg tgaccgttgc gcgcggcatc gagcggtgag gcgccgcgcg cgagcgacaa
     1141 caggaagccg gccgcgaacg cgtcgccggc accggtcgcg tcgatcggcc cgtcgaggac
     1201 caacgtggga atatctgcgc tgactgcgcc ccgcagcagg accggtgccg gtccctgttt
     1261 gacgaccacg tagcgcacgg tgccccaacc gtcgtcgtcg acaccgagcg ccgcggcctc
     1321 ttcgcggttg cacagcaaga cgtcgggctg cacgcgtacg agaagatcgc gcagacgatc
     1381 gtcgatcatc gtgaccgacg acggatcgac cgagcacagg atccctcgtt cgcgcgccgc
     1441 cgccaacatg gactccgcgg tacgagccag cggttgggtg acgaaggcgt acgcgggcac
     1501 gtgcaacacg gcgacgtcgt cgagccacgc gggctcgaac aggtcgagcg ccgacgaatc
     1561 gccgcggtcg ctgaacatcg tgcgttcgcc gtcgggttcg acgacgacga ccacgacgcc
     1621 ggtgcgcccc cggcgcgccc cgacgcagtc gacacctgcg ttcgccaact cggtgaggag
     1681 gtgcatgccg tcgtcgtcga cgccgacgca gccgacgaag cgggcccgtc cacccgcgtg
     1741 cacgacggcc gccgcggtgt tggccgcgct gccacctcgg tgtgccgtga tgtgcgcggg
     1801 cacgtcggcg tggcgcacca tcggctgcgc ggtgtggacg acgacgtccc ggacgagatc
     1861 gccgacggtg cacacgagcg gttccacggc tactgctata gcctgcgcgg ccgccatgcc
     1921 tcttaccgtc gtcgaccatc cactcgtcgc cgatcgaatc ctcacgctgc gggacgagcg
     1981 cacgaagtcc gtcgatttcc gccgcatcgc ccgcgaagtc gcgagcttcc tcgcctacga
     2041 atccacccgc gacctgccca ccgagcccga cgaggtcacg acgccgctcg gcctggtcgc
     2101 ccgcggcgtg cggccggccg tccccggtcc catcgtcgtg ccgatcctgc gggccgggct
     2161 cgggctgctg gacggcgtgc tcagcgcgct gcccgacgcc gaggtcggcg tgatcggcct
     2221 caagcgcgac gagaccacgc accggccgct cgcgtacctc gaacgcctcc ccgacgatct
     2281 ctcgggccgg cacgcgtaca tcgtcgaccc gatgctcgcg accggagggt cgctcgtgac
     2341 cgcagtcgag atgctgcgtt cgcgcggcgc gggcccgatc gtctgcctgt gtctcatcgc
     2401 cgcgcccgaa ggtgtcgagc tgctgcggaa ggcggatccc gacgtgcatg tcttcaccgc
     2461 cgccctcgac gacaagctca acgagcacgc gttcatcgtg cccggcctcg gcgacgccgg
     2521 cgaccggctg tacggggtgg ggtcggcgta gccctaacct tttgaggtta tggcgcgcct
     2581 gccgagatac cgcaccggtg atgccgagat cgacgagcag atcgcggatc tgatcgcgaa
     2641 gctcggcgac gacgtccgcg acaccgacct tctgttcgag ctcgtcgtgt cgaccgtccg
     2701 cctcgcccgt gaccacgcgt cgcgcggcga cctgaagatg gcgaacgcgg cactcaagga
     2761 gatgcgctac gcgttcgcag tgttcgagcc ctaccgctcg gcgcgcaagg ccgcgatctt
     2821 cgggtcggcg cgcacccgcc gtgacgatcc gctgtacgaa cagaccgtgc gattggcgga
     2881 agcgctcgcg gccgccgact ggatggtcat caccggcgcc ggccccggga tcatggaggc
     2941 cggcatcgag ggtgcggggg cggcgagctc gttcggtgtc ggcatccggc tgccgttcga
     3001 ggccgcgacg tcacagttcc tcgccgacga cccgaagctc gtcaacttcc gttacttctt
     3061 cacgcgcaaa gtcaccttcg tgaaggagtc gcacgcgttc atcctgctgc cgggcggctt
     3121 cggcacgctc gacgaggcct tcgagctgtt gacgctggtg cagacgggca agtcgcaacc
     3181 ggcaccgatc gtgctgctcg acgtacccgg cggcacctat tggctcacgt ggctcgagtt
     3241 cgtgcaccgc gagctgcgtg accgcggcta catctcggcc gaggacctcg atctcgtgaa
     3301 gatcaccgac gacgtcgacg tcgcgctggc cgaagtcacc ggcttctacc gcaacttcca
     3361 ttcgttgcgg ttcgtcgccg gtgatctcgt gctgcgcatc cacgagcagc cgaccgagcc
     3421 ggagctcgcc gcgctcaacg acgagttcgc cgacatcgtc tcgcgcggcc gcatcgagat
     3481 cgcggaggcg agcccggccg agatcgcgga tcgcgactac ccggagctcc cgcggctgcg
     3541 gttccgcttc gaccgccacg gattcgcgcg gctacggaag ctcatcgacc gcctcaacgc
     3601 gcccgagggc ctggatggcc ttcgcgatcg cccggcgggc ctgtaacaag cgcttctcc
//
LOCUS       DASUGA010000129         8419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1959673, whole genome shotgun sequence.
ACCESSION   DASUGA010000129 DASUGA010000000
VERSION     DASUGA010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8419
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1959673"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..756)
                     /locus_tag="VFX21_02500"
     CDS             complement(79..756)
                     /locus_tag="VFX21_02500"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX5094851.1"
                     /translation="MYYGTKRVFDCVAATMLVVIAFPLWIVLVVLIRLDSKGPALFKQ
                     QRVGSRRVVENGGTSWELVTFTFYKFRTMYVDEHEPAHRVYMTAYINNDIEYFSSRTG
                     GYADGESFRLRDDPRVTRIGRVLRKLSLDELPQLLNVLRGEMSMVGPRPPMPYEVALY
                     NDAQLQRLTARGGVTGWAQVNGRCTIGFDDVVRLDAEYARRQSFLFDLWILLRTVPAV
                     LSRRGAD"
     gene            925..1356
                     /locus_tag="VFX21_02505"
     CDS             925..1356
                     /locus_tag="VFX21_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094852.1"
                     /translation="MSADTVTLTILTGIWRTAMNEFRRRAEAIADRVSALIGHWGGYV
                     TLNDTEFRVWHPDKLRLDRAMAHVSGAYWYRPRLDQPHASGAWRIPVDNRIELVRALR
                     DFDPRRHKFVIDATTAQALQMRPQVRITTKLSRIFGHGGQH"
     gene            complement(1379..2248)
                     /locus_tag="VFX21_02510"
     CDS             complement(1379..2248)
                     /locus_tag="VFX21_02510"
                     /inference="COORDINATES: protein motif:HMM:NF020919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1996 domain-containing protein"
                     /protein_id="HEX5094853.1"
                     /translation="MTDRRTTMPLALAVLLVVLLASCKPVVPPPSRSSTELGSVRVEC
                     LPSHRAMVDPIVSWGQESAHVHDFFGNVSTNATSTYETMRDGGSSCSDPGDTAGYWIP
                     SLVAPDGRFVQPERAIFYYRNRPVEYSRTVPFRSNFRMIAGGTFPNTYWTCDGESDTG
                     FEDRRDFIPNCGPGRQIKVHVFFPSCWDGRRIDSADHRSHVTYGRDEDDGRVDGTDPD
                     VCPRTHPVKIPQLDFRVLYPVSDGRGYRFSDGALLPHSDFWNTWQRERLMHWVDSCLG
                     YEGRSCGLATEDR"
     gene            complement(2331..5900)
                     /locus_tag="VFX21_02515"
     CDS             complement(2331..5900)
                     /locus_tag="VFX21_02515"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HEX5094854.1"
                     /translation="MMISRKIATSVAALAIAAPSVAAVVAPVADAAAAAPAPSMAARE
                     VRTTWTSEIGVDRPEGVTYVPGRDELLVASPSETGTAVARLETDGEPKGDFALPPISD
                     VATLAYDPTSDQLAGVDGQDVVVAPAPTQGNAATTPVDGGADALAEPSGSSFDPATGT
                     WYVLDAGHNVIVASAEGAAPESEPQSIPMPQLAGKTLRGVAFNPQDGLLYVGSPDENL
                     LYAVDTSGAVVQSYDLTGLGLRDAGSMTFAPSADPTDAASTQHLYVADSGDTTQLGSV
                     MELTLAAEPVLAAAVVDDTGTLVQTIDTSKFSPGSPDPSGVTYLPGKDRLMLCDSEVE
                     ETTGAGYHGVNMWEINRNGAVTDTGTTYPAISKEPNGVGYIAASNTLLISDDSLRRIH
                     LMRPGPDNRFGTADDVVTFINAAAYGSDDTEDPTYDPVSGDIFFMDGVATEVFRVSPV
                     DGIFGNGNDTMTHFDVGQYGISDTEALEYYPPNDSLIVGNRTDRELIEVTKTGQLLRT
                     IDLSGVPGLKLVSGLTVAPASNGTGQLNYWIVDRGQDNGANSSENDGKLYEVAAGTVD
                     NTAPVVDSVVINQSAPKTNDTLSATVTAHDDDGDPLTYLYQWRKNGVSLTNQTGPTLD
                     LSIAGNGDKGDAISLRVNAFDGIVQSAPRTSAQVTVVNTNPVFGQNLLNRTDTAGATV
                     SFSAAATDADGDPLIYEATNLPSGVTINASSGAISGTIATGAAASSPYSVAVTVRDGA
                     APAATDTFTWTVNAAPPTNTAPVVDSVVINQSAPKTNDTLSATVTAHDNDGDPLTYTY
                     QWQRNGTNIPGGNAPSLDLSVAGHGNKGDQISVRVVASDGDLPSAPVTSAAVTIANSE
                     PVFSQNLANRTDAEDDLVVTSAAATDADNDALSYEATNLPGGIGINPATGVISGTITI
                     GAAASSPYSTAVTVRDGPTVDATDTFTWNVTAAPIVRSGAVTTTEGDTGSHIVTVPVT
                     LSHASASTVTVDWATTASVQPEPGVDFEAASGTVTFVPGDVNETVEITINGDVVDEPG
                     QLWGAEWGVIELSSPAHAALGAGPSDDLGLFLIVDDDPPPTIMPTSVAVTEGNSGSKI
                     ATVTVNLSAPSANTVTVNWATANSAQPEPGVDFVAASGAVTFLPGETTKTVPLTVLGD
                     TIDEPGNAWGAEWAAIVFSSPHNAKLGAQPFSNVSFVLIVDDD"
     gene            complement(6183..6254)
                     /locus_tag="VFX21_02520"
     tRNA            complement(6183..6254)
                     /locus_tag="VFX21_02520"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6219..6221),aa:Gln,seq:ctg)
     gene            complement(6262..6921)
                     /locus_tag="VFX21_02525"
     CDS             complement(6262..6921)
                     /locus_tag="VFX21_02525"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX5094855.1"
                     /translation="MGLRRLFRIARKVALGGLVLLVLYLGVTFVQVWRAARHEGKRPS
                     DAIVVLGAAQYDGVPSPVLAARLDHALDLYNEGIAPVIVVTGGRQEGDRFTEATAAAT
                     YLHNHGVPDDAIMRETTGKSSWESLAASARFLHEKGLNKVVLVSDPYHAKRIQAIAAE
                     VGLDAATSPTHTSPIKGSAEWRRFGNETLRVAAGRIFGYRRLDQSEQVGKLVRGLATM
                     L"
     gene            complement(6926..8350)
                     /gene="gltX"
                     /locus_tag="VFX21_02530"
     CDS             complement(6926..8350)
                     /gene="gltX"
                     /locus_tag="VFX21_02530"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX5094856.1"
                     /translation="MSSARVRFSPAPTGFMHIGNARTALFNWLFARKTGAPLILRIED
                     TDVSRSTSEAVEQIQVVLRWLGLEWDEGPFLQSSRFESYLAAADRLLAQGDAYECYCT
                     EEEVKQRNEEAMKAGRPPGYDGRCRNLTAEQRAAKSDAGLPRSVRFRTPDDGRSTFVD
                     IIRGEVSVEWSTIPDFVIVRSNGTPVFFLANALDDMEMGITHVLRGEDLIDSTHRVLA
                     LRHALGADDEPQYAHLPLIMGPGGGKLSKRHGATTVEEYRDGGILPHALVNYLALLGW
                     GPEDGREVLTVEELIAEFDLERVNPSAATFDPKKLEWMNGEHIRRMTVADLVAAVLPF
                     AKDRYGARLDVRVFDRAVALAQERATTLVQIAEQAEFLFTPDDELAIAPESWAQLEKV
                     DRVAELLDGTIARLEACEWTYDAIERELRAAVDDIDSKPRKAMPALYTAIEGRHAGLP
                     LFDSIELLGRESAVHRLRAARARL"
BASE COUNT         1329 a         2757 c         2869 g         1464 t
ORIGIN      
        1 cgggtagttc cgcgaacgtg cgcacgacgt tggggcccca gtatccgcat ccgatgacgc
       61 cgatgctgac ctgctccatc agtcagcacc ccttcgagac aacacggccg gcaccgtgcg
      121 cagcaggatc caaaggtcga agaggaacga ctggcggcgc gcatattccg cgtcgagacg
      181 gacgacgtcg tcgaacccga tagtgcaccg gccattgacc tgtgcccatc cagtcacgcc
      241 gccccgcgcc gtcaggcgct gaagctgcgc gtcgttgtac aaggcaactt cgtacggcat
      301 ggggggacgc ggaccgacca tgctcatctc accgcgcagc acgttcagca gttgcggcag
      361 ttcgtcgaga ctcagctttc gcaacacgcg gccgatccgg gtgactcgcg ggtcgtctcg
      421 caagcggaag gactcaccat ctgcgtagcc gccggtccgc gaagagaagt actcgatgtc
      481 gttgttgatg tacgccgtca tgtacacgcg gtgcgcgggc tcgtgctcgt ccacgtacat
      541 ggtccggaac ttgtagaacg tgaacgtcac gagctcccat gacgtaccgc cgttctcgac
      601 gactcgccgg ctgccgacgc gctgctgctt gaacagggcc ggtcccttgg agtccagccg
      661 tatgagaacg acgaggacga tccacaacgg gaaggcgatg acgacgagca ttgtggcggc
      721 cacgcagtcg aagacgcgct tggttccgta gtacatgcga gccgatcgcg cgacggccgc
      781 gtcgactcgg cgtacgggat ccagttgaag ggtcgtcgcc gtgctcactg ccgctgcact
      841 cctcggtcgg ggcgcttggc gagcgtatga gcgtggtcgg aagggatgat cgtgcagacg
      901 ccgtaggttg cctacgccgt gcgcatgagc gcagacaccg tcacgctgac gatcctcacg
      961 ggcatatgga ggacagcgat gaacgagttt cgtcgccggg cggaagcaat cgccgaccgc
     1021 gtcagcgcgc tgatcggaca ctggggcggc tacgtcacgc tcaacgacac cgagttccga
     1081 gtgtggcatc ccgacaaact ccgacttgac cgcgcgatgg cgcacgtgtc cggcgcctac
     1141 tggtatcgac cacgcctcga ccaaccccac gcgagcggcg cctggcgcat cccggtcgac
     1201 aacagaatcg aactcgtacg tgccctacgc gacttcgacc cacgacgcca caagttcgtc
     1261 atcgacgcga cgaccgcaca agcgctgcag atgcgaccac aagtgcgcat cacgacgaag
     1321 ttgagccgca tcttcgggca cggcgggcag cactgactcg ttcactcgcg cagcgacatc
     1381 acctgtcttc ggtggccagc ccgcacgatc ggccctcgta gcccaggcac gaatcgaccc
     1441 agtgcatcag ccgttcccgc tgccacgtat tccagaagtc actgtgcgga agcagcgcac
     1501 cgtcggagaa gcgatagccg cgaccgtcgg atacgggata gagcacgcgg aagtcgagct
     1561 gggggatctt gacgggatgc gttcgcgggc agacgtcggg gtcggtgccg tccacgcgac
     1621 cgtcgtcttc gtcgcgacca tacgtgacgt gactgcgatg gtcggccgag tcgattcggc
     1681 gtccgtccca gcacgacggg aagaatacgt gcaccttgat ctgtcgacca ggcccacagt
     1741 tgggaatgaa gtcgcggcga tcttcaaaac cggtgtcact ctcgccgtcg cacgtccagt
     1801 acgtgttcgg gaaggtgcca ccggcgatca tccggaagtt cgagcgaaac ggcactgtgc
     1861 ggctgtactc gaccggtcgg ttgcggtagt agaagatcgc acgttctggt tgcacgaacc
     1921 ttccgtcggg ggcgacgagc gacggtatcc agtagcccgc tgtatcgccc gggtccgagc
     1981 acgagctacc gccgtcacgc atcgtctcgt acgtcgacgt cgcgttggtt gagacgtttc
     2041 cgaagaagtc gtggacatgg gccgactcct gtccccatga aacgatcggg tcgaccatcg
     2101 cacggtgcga gggcaggcat tccacccgca ccgaacccag ttccgtcgac gaccttgatg
     2161 gcggcggtac gacgggcttg cagctcgcga gcagtacgac gagaagaacg gcgagcgcca
     2221 agggcatcgt cgtgcgacgg tctgtcatgg gggcctcccg cgaggtcgcg ccgtcctgga
     2281 tcactccggc cgtgccgtcc atcgtggcgc gaccctcgcg ggcagtactg ctagtcgtcg
     2341 tcgacgatca acacgaagct cacgttcgaa aacggttggg cacccaactt cgcgttgtgg
     2401 ggtgacgaga agacgatggc cgcccattct gcgccccaag cgtttccggg ctcgtcgatc
     2461 gtgtccccga gcacggtcaa cggcactgtc ttcgtggttt cgccgggcag gaacgtcact
     2521 gctcccgagg ccgcaacgaa gtcgacaccc ggctcgggct gcgcggagtt ggcggttgcc
     2581 cagttgaccg tcaccgtgtt cgcggaaggc gcggacagat tcaccgtcac ggtggcgatc
     2641 ttcgagccgg agttcccctc ggtcacggcg accgaggtcg gcatgatcgt cggcggcggg
     2701 tcgtcgtcga cgatcaggaa caggccgagg tcgtcactcg gcccggcccc gagcgcagca
     2761 tgcgccggcg acgacagttc gatgacgccc cattcggcgc cccagagctg tccgggctcg
     2821 tcgaccacgt ctccgttgat cgtgatctcg accgtctcgt tcacgtctcc gggtacgaat
     2881 gtgaccgttc ccgaagccgc ttcgaagtcg acacccggct ctggttgcac ggacgcggtc
     2941 gtggcccaat cgacggtcac ggtcgatgcg ctcgcgtgac tcagggtgac aggaaccgtg
     3001 acgatgtgcg acccggtgtc accttcggtc gtcgtgaccg cgcccgagcg cacgatgggc
     3061 gcggcggtga cgttccatgt gaacgtgtcg gtcgcgtcga ccgtcgggcc gtctcgaacc
     3121 gtgacggcgg tgctgtacgg actcgacgcg gcggcgccga tcgtgatcgt cccgctgatc
     3181 acaccggtcg cggggttgat gccgatccca ccgggcaggt ttgtcgcttc gtagctgagc
     3241 gcgtcattgt cggcgtcggt cgcggccgcc gacgtgacga cgaggtcgtc ttcggcatcg
     3301 gtgcgattcg cgaggttctg tgagaacacg ggctccgagt tcgcgatggt gacggccgcg
     3361 ctcgtcaccg gagcgctcgg taagtcgccg tcggacgcga cgacgcggac cgagatctga
     3421 tcacccttgt tgccatggcc cgcgacggag agatcgagcg aaggcgcgtt ccctcccggg
     3481 atgttggtgc cgttgcgctg ccactggtag gtgtacgtca gcgggtcgcc gtcgttgtcg
     3541 tgcgcagtga cggtcgccga cagcgtgtcg ttcgtcttcg gcgcagactg gttgatgacc
     3601 accgaatcga cgacgggcgc ggtgttggtc ggcggcgccg cattgacggt ccacgtgaac
     3661 gtgtccgtcg ccgccggcgc cgcaccgtcg cgcacggtga ctgcgacgct gtacgggctg
     3721 ctggcggccg cgccggttgc gatcgtgccg ctgatcgcgc cgctcgacgc gttgatcgtc
     3781 acgccggacg gcaggttcgt cgcctcgtag atcagtgggt cgccgtcggc gtcggtcgcg
     3841 gccgccgaga acgagacggt cgcccccgcg gtgtcggtgc ggttgaggag gttctgcccg
     3901 aagaccgggt tcgtgttcac gaccgtgacc tgagcgctcg tcctcggcgc gctctgcacg
     3961 atgccgtcga aggcattcac acgaagcgag atcgcatcgc ccttgtcgcc gttgcccgcg
     4021 atcgagaggt cgagcgtcgg cccggtctgg ttcgtgagcg agacaccgtt cttgcgccac
     4081 tggtacagat acgtcagcgg gtcgccatcg tcgtcgtgtg cagtcaccgt ggccgagagc
     4141 gtgtcgttcg tcttgggcgc ggactggttg atcacgaccg agtcgacgac cggtgcggtg
     4201 ttgtccacgg tgccggcggc gacttcgtac agcttgccgt cgttctcgct ggagttcgcg
     4261 ccgttgtcct ggccgcggtc gacgatccag tagttcagtt gtcccgtgcc gttgctcgcc
     4321 ggcgccaccg tcagcccgga cacgagcttg agaccgggaa caccgctcag gtcgatcgtg
     4381 cgcagcagtt gtccggtctt cgtgacctcg atcaactcgc ggtcggttcg attgccgacg
     4441 atcagtgagt cgttgggtgg gtagtactcg agcgcttcgg tgtcggagat gccgtactgc
     4501 cccacgtcga agtgagtcat cgtgtcattg ccgttgccga agatgccgtc gaccggactg
     4561 acgcggaaca cctctgtcgc aaccccgtcc atgaagaaga tgtcacccga gacggggtcg
     4621 taggtggggt cctcggtgtc gtcgctgccg tacgcggcgg cgttgatgaa ggtgacgacg
     4681 tcgtccgccg tgccgaaacg gttgtcggga cccgggcgca tcaggtggat gcggcgcaga
     4741 ctgtcgtcgg agatcagcag cgtgttgctc gccgcgatgt acccgacgcc gttcggttcc
     4801 ttcgagatcg ccggatacgt ggtgccggtg tcggtcacgg cgccgttgcg gttgatctcc
     4861 cacatgttca cgccgtggta cccggcaccg gtcgtctctt cgacctcgga gtcgcagagc
     4921 atgagccggt ccttgccggg gaggtacgtg acaccggacg ggtccgggct cccgggagag
     4981 aacttcgacg tgtcgatcgt ctgcaccaac gtcccggtgt cgtcgacgac cgccgcggcc
     5041 agcacgggtt cggccgcgag ggtcagctcc atgacgctgc ccagctgcgt ggtgtcgccg
     5101 ctgtccgcga cgtagagatg ctgcgtcgac gcggcgtcgg tgggatcggc gctcggtgcg
     5161 aaggtcatcg acccggcgtc gcgcaatccc aagccggtga ggtcatatga ctgcacaacg
     5221 gcgcccgacg tgtcgactgc gtagagcagg ttttcgtcgg ggctcccgac gtacaggagt
     5281 ccgtcttgcg gattgaacgc gacgccgcgc agtgtcttgc ccgcgagttg cggcatcggg
     5341 atcgactgcg gttcgctctc cggtgctgcg ccctcggcgg atgcgacgat cacgttgtga
     5401 ccggcgtcga gcacgtacca ggtgccggtg gccgggtcga acgaggaccc actcggctct
     5461 gcgagcgcgt cggcgccacc gtcgaccggc gtcgtggctg cgtttccttg cgtcggcgcg
     5521 ggcgcgacga cgacatcctg cccgtcgacc ccggcgagct gatcggaggt ggggtcgtac
     5581 gcgagcgtgg cgacgtcact gatcggcggg agcgcgaagt cgcccttcgg ttcgccgtcg
     5641 gtttcgagac gcgcgaccgc ggtgccggtc tcgctcggcg acgcgacgag gagctcgtcg
     5701 cgtccgggaa cgtaggtgac accctcgggt cgatcgacgc cgatctcgga cgtccatgtc
     5761 gtacgaacct cgcgggctgc catcgacggc gcgggtgcgg ccgcggctgc gtcggcgacg
     5821 ggcgcgacca ctgccgcgac ggaaggtgcg gcgattgcga gtgccgcgac cgacgtcgcg
     5881 atcttccggg aaatcatcat gaggccgcct cttggtgtcc atggaacgcg tcggacccta
     5941 aagacgtctt cgcgccgtgc attcacccgc gcaggcgagc gcgcctaccc caagtgccgt
     6001 agacgacacc tcagcaactt tggtgaggcg tcatcgccaa cattggcgat gcatcagccc
     6061 acaccggcgc cgtaccgtct cgttaccgtc ggctccccgg tgtcggcgat tggccccgga
     6121 ccgctcctcc gctgtccggg gccgacgaat tcgaagcgca ggttcgtgca cgtgcgcttg
     6181 cgctcgggag ggaggactcg aacccccaat aacagggcca gaacctgtcg tgttgccgat
     6241 tacaccactc ccgaagggac gtcacagcat agtcgcgagc ccgcgtacga gcttgccgac
     6301 ctgctcactc tggtcgagac ggcggtagcc gaagatgcgg ccggcggcga cgcgcaacgt
     6361 ctcgttgccg aagcgccgcc actcggccga acccttgatc gggctggtgt gcgtcggtga
     6421 cgtcgccgcg tcgaggccga cctcggccgc gatcgcctgg atgcgcttcg cgtgatacgg
     6481 gtcggagacc agcacgacct tgttcaggcc cttctcgtgc aagaagcgcg cggaggccgc
     6541 caacgactcc cacgacgact tgcccgtcgt ctcgcgcatg atcgcgtcgt cgggcacacc
     6601 gtggttgtgc aggtaggtcg cggccgcggt cgcttcggtg aagcgatcgc cttcttggcg
     6661 gccgccggtc acgacgatca ccggcgcgat gccctcgttg tacaggtcga gtgcgtggtc
     6721 gaggcgcgcc gcgagcaccg gcgacggcac accgtcgtat tgcgcggcgc cgagcacgac
     6781 gatcgcgtcg gacggccgtt tgccctcgtg acgcgcggcc cgccacacct gcacgaacgt
     6841 gacgcccagg tagaggacga gcaggacgag cccgccgagc gcaaccttgc gggcgatgcg
     6901 gaagaggcgt ctcaggccca tggcgtcaca gccgggcgcg ggccgcccgc aaccggtgca
     6961 cggccgactc gcgaccgagc aattcgatcg agtcgaagag cggcaggccc gcgtggcggc
     7021 cttcgatcgc ggtgtagagc gcgggcatcg ccttgcgggg cttcgagtcg atgtcgtcga
     7081 ccgcggcgcg cagctcgcgt tcgatcgcgt cgtaggtcca ctcgcacgct tcgagtcgcg
     7141 cgatcgtgcc gtcgagcagt tcggcgaccc ggtcgacctt ctcgagctgc gcccacgact
     7201 cgggtgcgat cgcgagctcg tcgtcggggg tgaacaagaa ctcggcctgc tccgcgatct
     7261 gcaccagcgt cgtggcgcgc tcctgcgcga gcgcgacggc ccggtcgaag acgcgcacgt
     7321 cgaggcgggc gccgtagcgg tccttggcga acggcaacac agccgcgacg aggtcggcga
     7381 cggtcatgcg gcggatgtgc tcgccgttca tccactcgag cttcttcggg tcgaacgtcg
     7441 ccgccgacgg gttcacccgt tcgagatcga actcggcgat cagctcctcg acggtgagca
     7501 cttcgcgacc gtcttcggga ccccagccca gcagcgcgag atagttcacg agcgcatgcg
     7561 ggaggatgcc gccgtcgcgg tactcctcca ccgtcgttgc accgtggcgc ttcgacaact
     7621 tcccgccgcc gggacccatg atcagcggca ggtgcgcgta ctgcggctcg tcgtcggcgc
     7681 cgagcgcgtg gcgcagcgcg agcacccggt gcgtcgagtc gatcaggtct tcgccccgca
     7741 agacgtgcgt gatgcccatc tccatgtcgt cgagcgcgtt ggcgaggaag aacaccggcg
     7801 tgccgttgga gcggacgatg acgaaatcgg ggatcgtcga ccactcgacc gacacttcac
     7861 cgcggatgat gtcgacgaac gtgctgcgcc cgtcgtcggg ggtgcggaac cgcaccgacc
     7921 gcggcaggcc cgcgtcggac tttgccgcgc gctgctcggc cgtgaggttg cgacaccgcc
     7981 cgtcgtagcc cggcgggcgc ccggccttca tcgcttcctc gttgcgctgc ttcacctctt
     8041 cttcggtgca gtagcactcg tacgcgtcgc cctgcgcgag gagccggtcg gcggctgcga
     8101 ggtacgactc gaaccggctg ctctgcagga acggaccttc gtcccactcg agcccgagcc
     8161 agcgcaacac gacttggatc tgctcgaccg cttcactcgt ggaccggctg acgtcggtgt
     8221 cctcgatgcg caggatcagt ggcgcgcccg tcttgcgcgc gaagagccag ttgaacagcg
     8281 ccgtgcgcgc gttcccgatg tgcatgaagc cggtcggggc cggagagaaa cgcacccgcg
     8341 cagaggacat cgaggcccat cgtacggggc cggtcggact gctcagggtt cgagtgcgaa
     8401 cagctcccag ggccgggcg
//
LOCUS       DASUGA010000130         2225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_465786, whole genome shotgun sequence.
ACCESSION   DASUGA010000130 DASUGA010000000
VERSION     DASUGA010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465786"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VFX21_02535"
     CDS             <1..448
                     /locus_tag="VFX21_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEX5094857.1"
                     /translation="FVIIDFEGEPARTIGERRLKRSPFRDVAGMLRSFDYAARSSIAE
                     VVARGTVRSEERAQELLAASAQEWVAWVSAAFLSGYFEAVSGQPFVPADRDACRVQLD
                     AHLLEKVLYEIGYELDHRPDWVGIPIGGLRALLEDESASMLVDARR"
     gene            445..>2225
                     /locus_tag="VFX21_02540"
     CDS             445..>2225
                     /locus_tag="VFX21_02540"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX5094858.1"
                     /translation="MSALPALARDLGIDVTWQTHAGDDARVREETLLALVNLLGAAVR
                     TPADADGARQAWHRQRMARVLEPTTVVWDSRTPRLRVYVPASQTDAPLECHLELDDGH
                     EWHAPVSKLPVDVMPVDVNGYVRLDVRLPTRLPTGVHTARVTGAGVSGASTVIATPSR
                     IPTNGVRRAWGVFAPLYALHDAVDDGPANLATLARFGAWAHGRGAQVVGTLPLLATYY
                     GAGNEPCDPSPYAPVSRRFWNEAYLDLDAVPGTAPRAGRALEGATIDPPALAADARAR
                     LEPLVEALRAQRAFQLWIERRDDVRAYASFRATTEGNPAARTYHEIAQWLVERQLHDL
                     VDEFDDRRQLLYLDLPLGSHPLGFDVHEGGDTFLRGATVGAPPDAFQPDGQDWGFTPL
                     HPDRARVTGHRHLRECLEAQFRFARLLRIDHVMGLDRLWIIPDGMAASDGAYVRYASE
                     EQWATVCLTANTSGAGVVGENLGTVPAATNRALRAHDGLGMWVLEFEVPEDATPATPR
                     ADALACCNTHDLDTFATWWDGLSPRRRARLAEVLRAADVLHLHEGEGLDPHDVLGACY
                     AWLGRSDAAIVLVTLEDLWLETEPQNRP"
BASE COUNT          322 a          781 c          787 g          335 t
ORIGIN      
        1 cttcgtcatc atcgacttcg aaggcgagcc ggcgaggacg atcggcgaac gccgcctcaa
       61 gcgatcgccg ttccgcgatg tcgccggcat gttgcgctcg ttcgattacg cggcccggtc
      121 ctcgattgcc gaagtggtcg cgcgcggcac cgtccgcagc gaggagcgag cccaggagtt
      181 gctcgcggcg agcgcgcagg agtgggtcgc ctgggtgtcg gccgccttcc tgagcggcta
      241 cttcgaggcg gtctccggtc agccgttcgt ccccgccgat cgtgacgcct gtcgcgtcca
      301 gctggatgcc cacctgctcg agaaggtgct gtacgagatc ggctacgagc tcgaccaccg
      361 gccggactgg gtcggcatcc cgatcggcgg tctgcgcgca ctgctggagg acgagagcgc
      421 atccatgctg gtggacgcgc gccgatgagc gcgctcccag cgctcgcgcg tgacctcggc
      481 atcgacgtca cctggcagac gcacgccggc gacgacgcac gggtacggga ggaaacactg
      541 ctcgcgctcg tgaacctcct cggcgcggcg gttcgcacgc cggccgatgc ggacggcgcg
      601 cggcaggcgt ggcatcgaca gcgcatggcg cgggtgctcg aaccgacaac agtggtgtgg
      661 gactcgcgaa caccgcggct gcgcgtgtac gtcccggcat cgcagaccga cgcaccattg
      721 gagtgccacc tcgaactgga cgacggtcac gaatggcatg cgcccgtgtc gaagctgcct
      781 gtcgacgtga tgcccgtcga cgtgaacggg tacgtccggt tggacgttcg gctcccgacc
      841 cgcctgccga cgggtgtgca caccgcacgc gtcacgggcg cgggcgtgtc gggtgcatcg
      901 acggtgatcg cgacgccgag ccggataccg acgaacggcg tacgccgcgc gtggggcgtg
      961 ttcgcgccgc tgtacgcgtt gcacgacgcg gtggacgacg gcccggccaa cctcgccacg
     1021 ctcgcccgtt tcggagcctg ggcgcacggg cggggcgcgc aggtcgtcgg cacgctcccg
     1081 ttgttggcga cgtactacgg agcgggcaac gagccgtgcg atccgagtcc gtacgcaccg
     1141 gtgagccgtc gcttctggaa cgaggcgtac ctcgacctcg acgccgtgcc gggtactgca
     1201 ccgcgggccg gccgtgcact cgagggcgcg acgatcgacc cgcccgcgct cgcggcggac
     1261 gcgcgcgccc gcctcgaacc tttggtcgag gcgctgcgcg cacaacgcgc gttccagctg
     1321 tggatcgagc ggcgcgacga cgtgcgggcg tacgcgtcgt ttcgggcgac gaccgagggc
     1381 aatccggccg cacgtacgta tcacgagatc gcgcagtggc tcgtggaacg acaactccac
     1441 gacctcgtcg acgagttcga cgaccgtcgc caactcctgt acctcgacct cccgctcggc
     1501 tcgcatcccc tcgggttcga cgtgcacgag ggcggcgaca cctttctgcg cggcgcaacg
     1561 gtcggcgcgc cgcccgacgc attccaaccc gatggccagg actggggctt tacgccattg
     1621 caccccgacc gggcgcgcgt caccggccac cgtcatctcc gtgagtgcct ggaggcgcag
     1681 ttccgcttcg ctcgtctgct gcgcatcgac cacgtgatgg gactcgatcg gttgtggatc
     1741 attcccgacg gcatggcggc ctcggatggc gcgtacgttc gctacgcgtc cgaggagcaa
     1801 tgggcgaccg tctgcctgac tgcgaatacg agcggtgccg gcgtcgtcgg tgagaacctc
     1861 ggcacggtgc cggcggcgac gaaccgggcc ttgcgcgcgc acgacggcct cggcatgtgg
     1921 gtcctcgagt tcgaagtccc cgaggacgcg acgcccgcga caccgcgcgc cgacgcgctc
     1981 gcgtgctgca acacgcacga tctcgacacg ttcgcgacat ggtgggacgg cctgtcaccg
     2041 cgccgccgcg cgcggctcgc cgaggtgctc cgcgccgcgg acgtcctgca cctgcacgaa
     2101 ggtgagggcc tcgacccgca cgacgtgctg ggcgcgtgct acgcgtggct cggccggtcc
     2161 gacgcggcga tcgtgctcgt gacgctggag gacttgtggc tcgagaccga accgcagaac
     2221 cgacc
//
LOCUS       DASUGA010000131         2612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1436586, whole genome shotgun sequence.
ACCESSION   DASUGA010000131 DASUGA010000000
VERSION     DASUGA010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436586"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFX21_02545"
     CDS             <1..297
                     /locus_tag="VFX21_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094859.1"
                     /translation="RAVATGRPEIVTAAEREHRRALGFPPFGALAELSGDDAAVQAAA
                     DALRDAGAVVLGPSDAKALVKMPTADDLAGLLDEPLDGARAVGRLRVAVDPPRV"
     gene            386..>2612
                     /locus_tag="VFX21_02550"
     CDS             386..>2612
                     /locus_tag="VFX21_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEX5094860.1"
                     /translation="MTHNRVHTESGPPTGHPGVAAPAIGSVWAEVLGLDAVAPEDDFF
                     ALGGDSLCALRAVTQIQEALGCQLDVSVLFEHPTFDEFVDAVIADAQAQATPALALEF
                     EPADRDGDLPLSFPQLRLWTGQRQIGETPFYNVPCSYRLRGALDRDAMQRALDAIVER
                     HEALRTCFPAAADGAAQQIIMAPAPVSMQEIDLRTEADPLAAARAWVASEARRPFAVE
                     TGPLLRTALLALADDDHVLALSMHHIVSDGSSMRVFGEELSALYGAFAAGSGSPLAPL
                     ALQYADFAAWQQRTFTDRVLAPHIEYWCDQLAGAETLELPVDRPRVLKRPYASGQFQF
                     ALSPEVVGGLRRIGRDENATLFMVLLAALQVTLSRTTGQTDISVGSPVANRNLAAAQK
                     LIGFFVNTLVLRTDCDGNPTFTELVRRARRVAVDAYAHQDLPFDRIVDELQPRPQPGY
                     NPLYQVHFQVVPMPLRDIKIGDLDAEFFESSSGATPLDLFVHCFESRDGVEGWVEFAA
                     DLFDEATIRRVLDHFAALCAAVAANPDCRVDDVVLGPPAAPLTGGAPVAGAPSVPALL
                     EERAGGSPEVWAVTGGESHLTYAQLAHTARRIARRLSDNGVHPGVAVGVCGAATVDGV
                     AAWFGALAAGARVVWLDPQDPPGWTSEVLAATGLRVVVATAATAHAVPAGPRVLVLDD
                     GPQPREPLEAVTTTAIAMLAPTLTGEGVRAVPVDHATLTGNARALQQLFPLQPRDRVL
                     HH"
BASE COUNT          351 a          953 c          924 g          384 t
ORIGIN      
        1 cgggctgtcg cgaccggccg gcccgagatc gtcaccgcgg ccgagcgcga gcaccggcgc
       61 gcgctcggct tccccccgtt cggcgcgctg gccgagttga gcggcgacga tgccgcggtg
      121 caggccgccg ccgacgcact ccgggacgcg ggcgcggtgg tgctcggccc gagcgacgcg
      181 aaagcgctgg tgaagatgcc cacggccgac gacctcgccg gcctgctgga cgagccgctc
      241 gacggtgccc gcgccgtcgg tcgcctccgg gtcgcggtcg acccgccgcg agtgtgactg
      301 gtgtcactgc cgggcgtcat cggcgagtgg ttctagatcc tgtcaaagag aaccgttagg
      361 gtcataccgt catagctggg cggtcatgac acacaaccgg gtacacacgg agagcggccc
      421 tccgaccgga caccccggcg ttgccgcgcc cgcaatcggt tccgtatggg ccgaggtgct
      481 cgggctggat gcggtggcgc cggaggacga cttcttcgcg ctcggcggcg actccctgtg
      541 cgcactgcgc gccgtcacgc agatccaaga ggcgctgggc tgccagctcg acgtctcggt
      601 gttgttcgag caccccacct tcgacgagtt cgtggacgcg gtgatcgccg acgcgcaggc
      661 acaggcgaca ccggcgctcg ccctcgagtt cgaaccggcg gaccgcgacg gcgacctgcc
      721 gctctcgttc ccgcaactgc ggctgtggac tgggcagcgc cagatcggtg agacgccgtt
      781 ctacaacgtc ccgtgcagct accggctgcg cggtgccctc gaccgcgacg cgatgcagcg
      841 cgcgctcgac gcgatcgtcg aacgtcacga ggcactgcgc acatgcttcc ccgcggccgc
      901 cgacggcgcc gcgcaacaga tcatcatggc gccggccccg gtatcgatgc aggagatcga
      961 cctgcggacg gaggccgacc cgctcgccgc ggcgcgcgcg tgggtcgcgt ccgaagcgcg
     1021 gcgaccgttt gcggtcgaaa ccggtccgct gctgcgcact gcgctgctcg cgctcgcgga
     1081 cgacgaccac gtgctcgcgc tctccatgca ccacatcgtc tcggacggat cgtcgatgcg
     1141 cgtgttcggc gaagagctct cggcgctcta cggggcgttc gccgccgggt ccggctcgcc
     1201 cttggcgccg ctcgcgctcc agtacgcgga cttcgccgcg tggcagcaac gcacgttcac
     1261 cgaccgcgtg ctcgcacccc acatcgaata ctggtgcgac cagctggccg gagcggagac
     1321 gctcgagctg ccggtcgacc ggccgcgggt gctcaagcga ccgtacgcat cgggacagtt
     1381 ccagttcgcg ctctcgcccg aggtcgtggg gggactgcgc cgcatcggtc gcgacgagaa
     1441 cgcgaccttg ttcatggtct tgctcgccgc gctgcaggtg acgctctccc gcacgaccgg
     1501 gcagaccgac atcagcgtcg ggtcgccggt ggccaatcgc aacctggcgg ccgcgcagaa
     1561 actgatcggg ttcttcgtga acacgttggt gctccgtacc gactgcgacg gcaacccgac
     1621 gttcaccgag ctcgtccgcc gcgcccggcg cgtcgcggtc gacgcgtacg cgcaccagga
     1681 cctgccgttc gaccgcattg tcgacgagct ccaaccgcgc ccgcagccgg gctacaaccc
     1741 gctgtaccag gtgcacttcc aagtggtgcc gatgccgctg cgcgacatca agatcggcga
     1801 cctcgacgcg gagttcttcg agagctcgtc gggcgccacg ccgctggacc tgttcgtgca
     1861 ctgcttcgag tcgcgcgacg gcgtggaggg gtgggtcgag ttcgccgccg acctcttcga
     1921 cgaagcgacg atccgccgcg tgctcgacca cttcgccgcg ttgtgcgcgg cggttgccgc
     1981 gaaccccgac tgccgcgtcg acgacgtcgt gctcggtccg ccggccgcgc cgctgacggg
     2041 cggcgcgcca gtcgcaggcg caccgtcggt gccggcgttg ctcgaggagc gcgcgggcgg
     2101 gagccccgaa gtctgggcgg tgaccggtgg cgagtcgcac ctgacctacg cgcagctcgc
     2161 gcacaccgcc cgccggatcg cgcggcggct gtcggacaac ggtgtgcacc ccggcgtcgc
     2221 ggtcggggtc tgcggcgcgg cgacggtcga cggggtcgcg gcatggttcg gtgcactggc
     2281 ggcgggcgcg cgcgtcgtct ggctcgatcc gcaggaccca cccgggtgga cgagcgaggt
     2341 gttggcggca accggtctgc gcgtcgtcgt cgcgacggcg gccaccgcgc acgcggtgcc
     2401 ggccggcccg cgtgtgctcg tgctcgacga cggcccgcaa ccgcgcgaac cgctcgaggc
     2461 ggtcactacg actgcaatcg caatgctcgc gccgacgctc accggcgagg gtgtgcgcgc
     2521 cgtacccgtc gaccacgcca cgctgaccgg gaacgcgcgt gcgctgcagc agctgttccc
     2581 gcttcagcca cgcgatcgcg tcttgcacca tc
//
LOCUS       DASUGA010000132         8721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_398123, whole genome shotgun sequence.
ACCESSION   DASUGA010000132 DASUGA010000000
VERSION     DASUGA010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8721
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398123"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VFX21_02555"
     CDS             <1..652
                     /locus_tag="VFX21_02555"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX5094861.1"
                     /translation="VVTVGTAANADYRIADVHVDRALTPSFSLEGVRLTVPLPGAHQV
                     QNAALAAAVAHRAFELSWDEIRDDLQSAVPARWRMEIAESTEGVIVLNDSYNANPASV
                     EAALRALAHLHTTGTRIAVLGDMRELGAHADDAHAGIGALAGELGIDVVIGVGPGGAQ
                     IVDAVDEPATALRAADAATARALVAEMVKPGDAVLVKASRALGLQVVAEALMEDAP"
     gene            649..1716
                     /gene="mraY"
                     /locus_tag="VFX21_02560"
     CDS             649..1716
                     /gene="mraY"
                     /locus_tag="VFX21_02560"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020646090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX5094862.1"
                     /translation="MIAMLMAAAAAFAITIFGTPFLIRFLQKRGVGQQIRDDGPIEHP
                     HVAKAGTPTMGGLAIVLAVVMGYLFAHLRRENVKFSSAGWTLLLIVVGLGLVGFIDDY
                     LGIIKRRNLGLRKRGKTLGIVLVALVFAFLAINWVHASTMLSFTRPFDFDFTTVGWVL
                     WTVFVVYAMANAVNFTDGADGLAAGSSAFTFGAFVVIGFTEFRHPEIYVRSGEAIPHD
                     LFSAQALDLAIIAAAMLGACAGFLWWNAAPAKIFMGDSGSLAIGGAMAGLALLTRTQL
                     LLPILAGLPLIEMLSVIAQIIAYRGFRRRVLRMAPIHHHFEVGGWSEFTVIVRFWLFA
                     GICAAAGIGIFYADFLRFGLE"
     gene            1713..3062
                     /gene="murD"
                     /locus_tag="VFX21_02565"
     CDS             1713..3062
                     /gene="murD"
                     /locus_tag="VFX21_02565"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX5094863.1"
                     /translation="MTAALVIGLAATGEAVARRLRAEGVDVVVTDDQPGGAAYDARSI
                     AVRDAGATLVESPSPADYDALVADTDLVVPSPLVAESHPAVQAARRRDVPVRSEIDLA
                     GERARVPIVAVTGTNGKTTVTTLTAAMLSASGVRAAAAGNIGRTLLDAVDDDVDVLVA
                     EVSSFQLAFAETFAPAVAILLAIADDHLDWHGSFDHYVWSKSRIFEHQRGKDLLVYDA
                     DDDIASRAAVHAPAGRVGVTRAARAATYHVSRGELRTPEGRALIAIKEMPRALTHDVT
                     NALAAAAAALDVGATPDGVHDTLASFATLPHRVTLVGEHRGVRYYDDSKATNPHAALS
                     ALASFESVVLIAGGRNKGLDLGVLATAADRIRAVVAIGEAAPEVIAAFDGVRPVTTAP
                     TMRDAVRTAAAASEPGDAVLLSPACASFDAYSGYAARGDDFAAEVRALIEDEGVVAR"
     gene            3059..4273
                     /gene="ftsW"
                     /locus_tag="VFX21_02570"
     CDS             3059..4273
                     /gene="ftsW"
                     /locus_tag="VFX21_02570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX5094864.1"
                     /translation="MTAVTPRIQIGRLRSRAAAMVRPRVAPARPPAFVVITATIAVLN
                     VVGLVMILSASSVAALSNYGSSWYFFNRQLAWALVGAVAFVVMSRVDYKRWRKLSPVI
                     LFGTLIALFVVLIPGIGIMVDGSRRWLGVGSMLRVQPSEIAKVALLLYAADVLTRRAD
                     SLANWYAWRPVLLVCGGLSALVLLEPDLDSTIVLGLITFALLLAAGVRARHLVNLAGI
                     GIGLSAILAISAPYRRARVFAFLDPWSDTSNTGYQIAQSLIALGSGGVSGVGLGAGRA
                     KWLFLPNAHTDFIFAIIGEELGLVGCLLVLGLFAGFGLCGFHIAKHAPDRFGALLAAG
                     ITTWIVGQAVINLGAVVGLLPVSGITLPFLSAGGSSLVVAMAAAGMLANVARQAVPAP
                     ARAREATAAPAE"
     gene            4277..5371
                     /locus_tag="VFX21_02575"
     CDS             4277..5371
                     /locus_tag="VFX21_02575"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEX5094865.1"
                     /translation="MGTFALIAGGGTGGHLYPAIALADELVARGHPRDGIRFVGGTRG
                     PEGRVVPEAGYAIDLLPGRGLQRKLTPANIVINVTVVFQTLTAMIRAIGLVRKYRPRV
                     VVGFGGYASLPCVVAAWCWRVPRVVHEQDSVMGLANRIGVRLGARVAASLPGVDAPGA
                     ELTGNPVRNEFRDLTRAPQSPPLVAAFGGALGAASINRAMLDCYDRWRDRSDIAVHHV
                     VGERNFADCDARLRSLRRVGDRLDYSLVAYEQRMNQLYERAALAVCRAGAGTVAELTA
                     AGLPAVLVPLPGAPSDHQSHNAATLEQAGAAIRLADEECDGAHLDALVGRLLADPGRL
                     QQMSAAARALARPDAAARVADLVEEHARGR"
     gene            5361..6698
                     /gene="murC"
                     /locus_tag="VFX21_02580"
     CDS             5361..6698
                     /gene="murC"
                     /locus_tag="VFX21_02580"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX5094866.1"
                     /translation="MAGDGYAGELDLSAPREIHIVGLAGVAMSAIALLLARLGHRVSG
                     SDIKDTPQLARLEAAGVTVHIGNRAEYVPPHADAVIYSTAIPENNVELVAARSLGIPV
                     LHRSLAQAAIAATRRTIAVAGSHGKTTTSSMLALVLRAAGWQPSFFVGGELNEVGTNA
                     AVGDGEWFVVEADESDGTFLQLAPEAAMVTNIEPDHLDYYGDFDTLVAAFERFVDAVP
                     GAVVMGADDEVAARLARSHPAVRTYGLAEGADYRMVGLRTETNTYAFTLAHDGAAVGE
                     IVVPLGVMAATNSAGAAAMALEIGVPFDAIRQALRGFGGVARRFQYRGERDGVTFVDD
                     YAHLPTEVAAAIQTARQGGWEHVIVVFQPHRYTRTAALGADFADAFTGADAVVVTDVY
                     AAGETPIAGVSGRLVAQAAADRHPELPLSYVPGRAELVDVPARFARRGDVVLT"
     gene            6758..7687
                     /gene="murB"
                     /locus_tag="VFX21_02585"
     CDS             6758..7687
                     /gene="murB"
                     /locus_tag="VFX21_02585"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX5094867.1"
                     /translation="MTLDATIAALEGALPGAVERAAPSAALTTYRCGGPLAARVLVEN
                     EDQLLRVAEIVRTDRVPVLVIGRGSNLLVADAGFAGVALSLAGDFEQFDVDTRAAHVN
                     AGGAVALPVLARRAAAAGVGGVEFFVGIPGSVGGAVRMNAGGHGRETRDVFVSARVCD
                     LDQGQTRELTLDDLRFGYRRSALTSRHVVVSATFEGAQADPAVCTARIDEIVRWRREH
                     QPGGQNAGSVFTNPPNDAAGRIIDACGLKGLSVGGASVSAKHANFFVAEPGATADDVH
                     ALVCTVQRQVELMTGVRLHPELQFVGFDSEGSA"
     gene            7684..8484
                     /locus_tag="VFX21_02590"
     CDS             7684..8484
                     /locus_tag="VFX21_02590"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX5094868.1"
                     /translation="MTPPDVKDGTRAPTRDEIEVGVDIDPRFQERREEVQREASEKRR
                     RILVLVAGGFVALGLLYLLVQSPMLDVDHVNVDGNHHVTADQVREAAGIDLGSPILLL
                     DTDAIAKRVRALPWVEEVDVQRKWSGTLSISVVEEEPVAWVSAGNGVALLSSEGRVIA
                     DVDVAPADTTEIKGVRKAPAIGKLLSPPEAAGAVRALPSQLANQVTAIDVGGTGVALE
                     LARGAEVRLGNLNDLDAKAAAAIAVLERWGDAPLTYIDVSVPQNPTAR"
BASE COUNT         1176 a         3084 c         3075 g         1386 t
ORIGIN      
        1 cgtcgtgacg gtcggcacgg cggcgaacgc cgactatcgc atcgccgacg ttcacgtcga
       61 ccgcgcgctc acgccgtcgt tctccctcga aggcgttcgg ctcacggtcc cgttgcccgg
      121 cgcgcaccag gtgcagaacg cggcgctggc cgcggcggtc gcgcaccgcg ctttcgagct
      181 gtcgtgggac gagatccgcg acgatctgca gagcgcggtt ccggcccgct ggcggatgga
      241 gatcgccgag tcgaccgagg gcgtcatcgt cctcaacgac tcgtacaacg cgaatccggc
      301 gtcggtcgaa gccgcattgc gcgcgctggc ccacctccac acgacgggca cccgcatcgc
      361 ggtgctcggt gacatgcgcg agctcggcgc ccacgccgac gacgcgcacg cgggcatcgg
      421 cgcactcgcg ggcgagctcg gcatcgacgt cgtgatcggt gtcggccccg gtggcgcgca
      481 gatcgtcgac gcggtcgacg aaccggcgac cgcgctgcgg gcggccgacg cggcgaccgc
      541 gcgggcactc gtcgcggaga tggtgaaacc cggcgacgcg gtactggtca aagccagtcg
      601 tgcgctcggg ctccaagtcg tcgccgaagc gctgatggaa gacgccccat gatcgcgatg
      661 ctcatggcgg cggccgccgc gttcgcaatc acgatcttcg gcacgccgtt cctcatccgc
      721 ttcctccaaa agcgcggcgt cggtcagcag atccgcgacg acggaccgat cgagcatccg
      781 cacgtggcca aggccggtac gccgacgatg ggtggcctcg cgatcgtgct cgcggtggtg
      841 atgggctacc tgttcgcgca cctgcgccgc gagaacgtga agttctccag cgcggggtgg
      901 acactgctcc tgatcgtcgt cgggctcggc ctggtcgggt tcatcgacga ctacttgggg
      961 atcatcaagc ggcgcaacct cgggttgcgc aagcggggca agacgctcgg catcgtgctc
     1021 gttgcgctcg tgttcgcctt cctcgcgatc aactgggtgc acgcgtcgac gatgttgtcg
     1081 ttcacgcgcc cgttcgattt cgacttcacg acggtcgggt gggtgctgtg gacggtgttc
     1141 gtcgtgtacg cgatggcgaa cgcggtcaac ttcaccgacg gggccgacgg cctcgcggcg
     1201 gggtcgtcgg cgttcacgtt tggcgcgttc gtcgtcatcg ggttcaccga gttccggcac
     1261 cccgagatct acgtgcgcag cggcgaggcg attccgcacg acctcttctc cgcgcaggcc
     1321 ctcgacctcg cgatcatcgc cgcggccatg ctcggcgcct gtgcgggctt cctgtggtgg
     1381 aacgcggcgc ccgcgaagat cttcatgggc gactcgggct cgctggcgat cggcggcgcg
     1441 atggcggggc ttgcactctt gacccgcacc cagttgctgc tgccgattct cgccggtctg
     1501 ccgttgatcg agatgctgtc ggtgatcgcg cagatcatcg cctaccgcgg gttccggcgc
     1561 cgtgtgttgc gcatggcgcc gatccaccat cacttcgagg tgggcggctg gtcggagttc
     1621 acggtgatcg tgcgcttctg gctcttcgcc ggcatctgtg ccgcggccgg catcggcatc
     1681 ttctatgccg acttcctccg gttcggtctc gaatgacggc cgcactcgtg atcggtctcg
     1741 cggcgacggg tgaagctgtc gcgcggcggt tgcgcgccga aggcgtcgac gtcgtcgtga
     1801 ccgacgacca gcccggcggt gccgcatatg acgcgcgctc gatcgcagtg cgcgacgccg
     1861 gcgcgacgct cgtcgagtca ccgtcgcccg ccgactacga cgcgctggtg gccgacaccg
     1921 acctcgtcgt gccgagcccg ctcgtcgccg agtcgcatcc ggccgtgcag gccgcgcgcc
     1981 gacgcgacgt gccggtgcgc agcgagatcg acctcgccgg cgaacgcgcc cgcgtgccga
     2041 tcgtcgccgt cacgggcacc aacggcaaga cgaccgtcac gacgctgacc gccgcgatgc
     2101 tcagcgcctc gggcgtccgg gcggcggcgg ccggcaacat cggccggacc ctgctcgacg
     2161 cggtcgacga cgacgtcgac gtgctcgttg ccgaggtgtc gtcgttccag ctcgccttcg
     2221 ccgagacgtt cgcgccggcc gtggcgatcc tgctcgcgat cgccgacgat catctcgact
     2281 ggcacggctc gttcgaccac tacgtctggt cgaagtcgcg catcttcgag caccaacgcg
     2341 gcaaggacct gctcgtctac gacgcggacg acgacatcgc atcgcgcgcc gcggtgcacg
     2401 caccggcggg gcgcgtgggc gtgacgcgag ctgcgcgcgc cgcgacatac cacgtgagtc
     2461 gcggcgagct gcggaccccc gagggccgcg cgctgatcgc gatcaaggag atgccgcgcg
     2521 cgctgacaca cgacgtcacg aacgcgctcg cggcggccgc cgcggcgctc gatgtgggcg
     2581 cgacgccgga cggcgtccac gacacgctgg cgtcgttcgc gaccctccct catcgcgtca
     2641 cgctggtcgg cgaacataga ggggtgcggt actacgacga ctcgaaggcg acgaaccccc
     2701 acgccgccct cagcgcgctc gcgtccttcg agtcggtcgt gctcatcgcg ggtggccgca
     2761 acaagggcct cgacctcggt gtgctcgcga ccgccgccga ccgcatccgc gccgtcgtcg
     2821 cgatcggcga agccgcgccg gaagtgatcg ccgccttcga cggcgtgcgg cccgtcacca
     2881 ccgcaccgac gatgcgcgac gcggtccgca ccgccgcggc ggcgtccgaa ccgggcgatg
     2941 ccgtcttgtt gtcgccggcc tgcgcgtcat tcgacgcgta cagcggttac gcggcgcgtg
     3001 gcgacgactt cgccgcggaa gtgcgcgccc tgatcgaaga cgaaggcgtc gtcgcccggt
     3061 gaccgccgtc acgccccgca tccagatcgg ccggctgcgc agccgcgcgg cggcgatggt
     3121 gcggccgcgc gttgcgccgg cgcgcccgcc cgcgttcgtc gtcatcaccg cgacgatcgc
     3181 ggtgctgaac gtcgtcggac tggtgatgat cctctccgcg tcgtccgtcg cggcgctgag
     3241 caactacggc tcgtcgtggt acttcttcaa ccgccaactc gcgtgggcac tggtcggcgc
     3301 ggtcgcgttc gtcgtcatgt cacgcgtcga ctacaagcgc tggcgcaagt tgtcgccggt
     3361 gatcctgttc ggcacactca tcgcgctgtt cgtcgtgctc atcccgggca tcggcatcat
     3421 ggtcgacggg tcgcggcgct ggctcggcgt gggcagcatg ctgcgcgtcc aaccgagtga
     3481 gatcgcgaag gtcgcgctcc tgttgtacgc ggccgacgtg ctcacccggc gcgccgactc
     3541 gctcgcgaac tggtacgcgt ggcgaccggt cctcctcgtc tgcggcggcc tgtccgcgct
     3601 cgtgctgctc gagcccgacc tcgattcgac gatcgtgctc ggcctcatca ccttcgcact
     3661 gttgctggcg gccggcgtgc gggcccggca tctggtgaat ctggccggca tcgggatcgg
     3721 cctgtccgcg atcctcgcca tctccgcgcc ctaccgccgg gcccgggtgt tcgcgttcct
     3781 cgacccctgg tcggacacgt ccaacaccgg ctaccagatc gcgcagtcgc tgatcgcgct
     3841 cggcagcggc ggcgtgagcg gcgtcggcct cggcgcgggc cgcgccaagt ggttgttcct
     3901 gccgaacgcg cacaccgact tcatcttcgc gatcatcggc gaggagctcg ggctcgtcgg
     3961 ttgtctgctc gtactcggat tgttcgcggg cttcgggttg tgcgggttcc atatcgcgaa
     4021 gcacgcgccc gatcgtttcg gcgcgttgct cgcggccggc atcacgacgt ggatcgtcgg
     4081 tcaggccgtc atcaacctcg gcgcggtcgt cggtctgctg ccggtgtcgg gcatcacctt
     4141 gccgttcctg tcggccggtg ggtcgtcgct cgtcgtcgcg atggccgcgg ccggcatgct
     4201 cgcgaacgtc gcgcgccaag cagtccccgc accggcacgc gcacgcgagg caactgccgc
     4261 acccgcggag taacacgtgg gtaccttcgc tcttattgcc ggcgggggca ccggtggcca
     4321 cctgtatccc gcgatcgcgc tcgccgacga gctcgtcgcg cgcggtcacc cgcgcgacgg
     4381 catccgtttc gtcggcggca cccgcggtcc cgaaggccgc gtcgtccccg aggccggcta
     4441 cgcgatcgac ctgttgcccg gtcggggact gcaacgcaag ctcacgccgg ccaacatcgt
     4501 catcaacgtc acggtcgtct tccagacgtt gaccgcgatg atccgcgcca tcggcctcgt
     4561 gcggaagtac cggccgcgcg tcgtcgtcgg cttcggcggg tacgcctcgc tgccgtgtgt
     4621 cgtggccgcg tggtgttggc gggtgccgcg cgtcgtccac gaacaagact ccgtcatggg
     4681 cctcgccaac cggatcggtg tccgccttgg tgcgcgcgtc gccgcgtcac tgccgggtgt
     4741 cgacgcaccg ggcgcggagc tgaccgggaa tccggtccgc aacgagttcc gtgatctgac
     4801 gcgcgcaccg cagtcaccgc cgctggtcgc cgcgttcggc ggcgcgctcg gcgccgcgtc
     4861 gatcaaccgg gcgatgctcg actgttacga ccgttggcgt gaccggtccg atatcgccgt
     4921 gcaccacgtc gtcggcgaac gcaacttcgc cgattgcgac gcgcggctgc gctcgttgcg
     4981 gcgcgtcggc gatcggctcg actattcgct tgtcgcgtac gagcaacgca tgaaccagct
     5041 ctacgagcgc gccgctctcg cggtgtgccg cgccggtgcc ggcacggttg ccgagttgac
     5101 ggccgcgggc ctgcccgcgg tgctcgtccc gctgccgggc gcgccgagcg atcaccagtc
     5161 gcacaacgcg gcaacgctcg agcaggcggg cgccgcgatc aggctcgccg acgaggagtg
     5221 cgacggcgcg cacctcgacg cgctggtcgg caggctgctc gccgatcccg ggcggttgca
     5281 gcagatgtcg gcggcggcgc gcgcgctcgc gcgacccgac gccgcggccc gggtcgccga
     5341 tctcgtcgag gagcacgccc gtggccggtg acgggtacgc gggcgagctc gacctctcgg
     5401 cgccgcgcga gatccacatc gtcggcctcg cgggggtggc gatgagcgcg atcgcactgt
     5461 tgctcgcccg cctcgggcat cgcgtcagcg gctccgacat caaggacacg ccgcagctgg
     5521 cgcgcctcga ggcggccggc gtcacggtgc acatcggcaa ccgcgcggag tacgtgccac
     5581 cgcacgcgga cgcggtcatc tactccacgg cgatccccga gaacaacgtc gagctcgttg
     5641 ccgcgcgctc gctcgggatc ccggtgttgc atcgctcgct cgcacaggcc gcgatcgcgg
     5701 cgacgcggcg caccattgcg gtggcgggtt ctcacggcaa gacgacgacg tcgtcgatgc
     5761 tcgcgctcgt gctgcgtgcc gcggggtggc agccgagctt cttcgtgggc ggcgagttga
     5821 acgaagtcgg cacgaacgcg gcggtcggtg acggcgagtg gttcgtcgtc gaggccgacg
     5881 agagcgacgg cacgttcctc cagctcgcgc cggaagcggc gatggtgacg aacatcgagc
     5941 ccgaccatct cgactactac ggcgacttcg acacgctcgt cgccgcgttc gaacgcttcg
     6001 tcgacgcggt gcccggtgcc gtcgtcatgg gcgcggacga cgaggtcgcg gcccgcctcg
     6061 cgcgcagcca ccccgcggtg cgaacgtacg gcctcgcgga gggcgccgac taccgcatgg
     6121 tcggcctgcg gaccgagacc aacacctatg cgttcacgct ggcgcacgac ggtgccgcag
     6181 tcggcgagat cgtcgtgccc ctcggcgtca tggccgcgac caacagtgcc ggcgcggcgg
     6241 cgatggcgct ggagatcggg gtgccgttcg atgcgattcg ccaggcgctg cgaggcttcg
     6301 gcggggtcgc ccgccgcttc caataccggg gcgagcgcga cggcgtgacg tttgtcgacg
     6361 actacgccca tctgcccacc gaggtcgcgg cggccatcca aaccgcgcgc cagggcgggt
     6421 gggaacatgt catcgtcgtg ttccaacccc accgctacac gcgcacggcc gcgctcggcg
     6481 cggacttcgc ggacgcgttc accggtgccg acgcggtcgt cgtcaccgac gtctacgccg
     6541 cgggcgagac gccgatcgcg ggtgtcagcg ggcggctcgt cgcgcaggcc gcggccgacc
     6601 gtcatcccga gctgccgttg tcgtacgtac ccggccgcgc ggagctggtc gacgtcccgg
     6661 cgcgcttcgc gcggcgcggc gacgtcgtgc tgacataggg tgcgggtgac atcacgacac
     6721 tgcccgacgt ctggctctcg accggcgaag gtcgctcgtg acgctcgacg cgacgatcgc
     6781 tgcgctcgaa ggcgcgctcc cgggcgcggt cgaacgcgcg gcgccgagtg cggcactcac
     6841 gacgtaccgc tgcggcggtc cgctcgcggc gcgcgtgctc gtcgagaacg aagaccagtt
     6901 gctgcgcgtg gccgagatcg tgcgcaccga tcgggtgccc gtgctcgtga tcgggcgcgg
     6961 ttcgaacctg ctcgtcgccg atgcggggtt cgcgggggtc gctctgtcgc tcgccggcga
     7021 cttcgagcag ttcgacgtcg acacgcgtgc cgcgcacgtc aacgcgggag gcgcggtcgc
     7081 gctgccggtg ctcgcccgcc gggcggcggc cgccggtgtc ggcggcgtcg agttcttcgt
     7141 ggggatcccc ggttcggtcg gcggcgcggt gcggatgaac gcgggcggcc acgggcgcga
     7201 gacgcgggac gtgttcgtca gtgcccgcgt gtgcgatctc gatcagggcc agacccgcga
     7261 gctgacgctc gacgacctcc ggttcggcta ccggcggtcg gcgctgacga gccggcacgt
     7321 cgtcgtgagc gcgacgttcg aaggcgcgca ggccgacccg gccgtgtgca cggcccgcat
     7381 cgacgagatc gtccggtggc gccgggagca ccagcccggc ggccagaacg cgggctcggt
     7441 cttcaccaac ccgcccaacg acgcggccgg ccgcatcatc gacgcctgcg ggctcaaggg
     7501 cctgtcggtg ggcggtgcgt cggtgtcggc caagcacgcc aacttcttcg tcgccgaacc
     7561 gggcgcgact gccgacgacg tccacgcgct cgtgtgcaca gtgcaacggc aggtcgagct
     7621 catgacgggt gtgcggctgc accccgaact gcaattcgtc ggtttcgact cggagggatc
     7681 ggcatgacgc cgccggatgt caaggacggg acccgtgccc cgacgcgcga cgagatcgag
     7741 gtcggggtcg acatcgatcc gcgcttccaa gagcggcgcg aggaagtgca gcgcgaggcg
     7801 agcgagaagc ggcgccggat cctcgtgctc gttgccggcg ggttcgtcgc gctcgggttg
     7861 ctgtacctgc tcgtgcaatc accgatgctc gacgtcgacc acgtcaacgt ggacggcaac
     7921 caccacgtca ccgccgatca ggtccgcgag gcggcgggca tcgacctcgg ctcgccgatc
     7981 ctgttgctcg acaccgacgc gatcgcgaag cgggtccggg cgctgccgtg ggtggaggag
     8041 gtcgacgtgc aacggaagtg gtcgggcacg ctcagcatct cggtcgtcga agaggaaccg
     8101 gtggcctggg tcagcgccgg caacggcgtc gcgctgctgt cgagcgaggg ccgcgtgatc
     8161 gccgacgtcg acgtcgcacc cgcggacacg accgagatca agggtgtgcg caaggcgccc
     8221 gcgatcggga agttgttgtc gccgccggaa gccgcgggcg cggtgcgggc gctcccgtcg
     8281 caactcgcga accaggtcac cgcgatcgac gtcggcggca ccggcgtggc gctcgagctc
     8341 gcgcgcgggg cagaggtgcg cctcggcaac ctcaacgatc tcgacgcaaa ggcagccgcc
     8401 gcgatcgcgg tcctcgaacg atggggcgac gcgcccctca cgtacatcga cgtgtcggtc
     8461 ccgcagaacc caaccgcgcg ttagaccgcg cgtgcgcgca ttctcctcac gccgagtaat
     8521 tgaatgcaca ccttcactga agtacagtct cggcatatac aacaggttga cataacgctc
     8581 aacctgaagt tgaggtcgag ggttcgagga agaaccccag cccaacgctc tgccaggtcc
     8641 actctttcgc acacttcacc ggcaatgctc gcgacgtgcg cacgtctcgt cagggttgaa
     8701 caggaaggtc ctccatgctc g
//
LOCUS       DASUGA010000133         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1021132, whole genome shotgun sequence.
ACCESSION   DASUGA010000133 DASUGA010000000
VERSION     DASUGA010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021132"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="VFX21_02595"
     CDS             complement(<1..1100)
                     /locus_tag="VFX21_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094869.1"
                     /translation="MKRALLVVAALVFALIVLVPMISAGPASAQGASTDQALLERFAP
                     VIAIRHQDTTCGSGERFRPVSVDVVLGRDDVVLRDGNGDVVRKAPTADDLAGLDDHHW
                     LDFPGNALKPGCTYEKWFKSLDATPSIYGRVTQDGGHLVLQYWFYWVFNQWNDVHESD
                     WEGIQLQFDTDDVDEAMTAKPSVYAYAQHEGAERADRGDDKVKLVDTHPVVYAAEGSH
                     ASYYSSTRWFGKSGATGFGCDDTRSPIDRLEPAVIRLPHDAPTSGQFAWLSYEGHWGQ
                     QEPFFNDGPTGPATKIRWDEPLTWVDDNGRDNSVTVPFARSGATDMFCSVSATGSALF
                     NDMLDNPVRAVVFVVFLLVAVVLVIRLGSRGLL"
     gene            complement(1097..2062)
                     /locus_tag="VFX21_02600"
     CDS             complement(1097..2062)
                     /locus_tag="VFX21_02600"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5094870.1"
                     /translation="MRKVFAPGTFDATDALLAERVRTAGLSGGIVRVVDFDGDLIHEH
                     AVGSVSGATPLSVGSSTKWLTAATFMTFVDRGAIALDDNIARWLPEFAAQDPPITPRM
                     LLDHTSGVHDNPCQDGGALAACVRTLAVSPREFRAGTAFSYGNAPFLVVGRLIEELGG
                     ADFATVVHDRLTGPLDMHDTTWPGAPSAANGAFGVLVTVDDYGKLLAMILHDGVANGA
                     RVLSSEAVRQLVSDQVGAYDTSHDYSVGITKIPRYGLGCWPDVVDASGRTLVVSGNGG
                     KGLYPWVDFTTRTWGIVGVQDERGAQVAVPASQVVEVEARTAVAR"
BASE COUNT          357 a          767 c          736 g          322 t
ORIGIN      
        1 agcagcccgc gtgacccgag gcggatcacc aggaccacgg ccacgagcaa gaacaccacg
       61 aacacgacgg cgcgcaccgg gttgtcgagc atgtcgttga acaacgccga acccgtcgcg
      121 ctgacgctgc agaacatgtc cgttgcaccc gagcgcgcga agggcaccgt caccgagttg
      181 tcgcgaccgt tgtcgtcgac ccacgtcaac ggttcgtccc atcggatctt ggtcgcggga
      241 cccgtgggac cgtcgttgaa gaacggctcc tgctggcccc agtgcccctc gtacgacaac
      301 cacgcgaact ggccgctggt cggcgcatcg tggggcagcc ggatcaccgc cggctcgagc
      361 cgatcgatcg gacttctcgt gtcgtcacaa ccgaaccccg tcgcgccgct cttcccgaac
      421 cagcgcgtcg acgagtagta cgacgcgtgc gacccctccg ccgcgtagac gaccggatgc
      481 gtgtcgacga gcttcacctt gtcgtcgccg cgatcggccc gctcggcacc ttcgtgctgt
      541 gcgtaggcgt acaccgacgg cttcgcggtc atcgcctcgt cgacgtcgtc ggtgtcgaac
      601 tgcagctgga tcccctccca gtcgctctcg tgcacgtcgt tccactggtt gaacacccag
      661 tagaaccagt actgcagcac gaggtgtccc ccgtcctggg tcacgcgccc gtagatggac
      721 ggcgtcgcgt cgaggctctt gaaccacttc tcgtaggtac agccgggctt caaggcgttc
      781 ccggggaagt cgagccagtg gtggtcgtcg agtccggcga gatcgtcggc ggtcggcgcc
      841 ttcctcacga catcgccgtt gccgtcgcgc aggacgacgt cgtcgcgtcc gaggacgacg
      901 tcgacggaga ccggacggaa ccgctcgccc gatccacacg tggtgtcctg gtgacggatc
      961 gcgatcaccg gggcgaagcg ctcgaggagc gcctggtcag tggacgcgcc ctgtgccgac
     1021 gccgggcccg cgctgatcat cggcacgagc acgatgagcg cgaacaccag cgcggcgaca
     1081 acgagtagcg cgcgcttcat cgggccaccg cggtgcgcgc ctcgacttcc acgacctggg
     1141 acgcggggac cgccacctgc gcgccgcgct cgtcctggac gccgacgatc ccccacgtgc
     1201 gcgtcgtgaa atcgacccag ggatacaagc ccttgccgcc gttgccgctc acgacgaggg
     1261 tccgtcccga cgcatcgacg acgtcgggcc agcagccaag gccgtagcgc gggatcttcg
     1321 tgatgccgac cgagtagtcg tgcgacgtgt cgtacgcgcc gacctggtct gacacgagct
     1381 ggcgtaccgc ctcggatgac agcacgcgtg cgccgttcgc cacgccgtcg tgcaggatca
     1441 tcgcgaggag cttcccgtag tcgtcgaccg tcaccagcac gccgaacgcg ccgttcgcgg
     1501 ccgacggcgc accgggccac gtggtgtcgt gcatgtcgag cggaccggtg aggcgatcgt
     1561 ggacgaccgt cgcgaagtcg gcgccgccga gctcctcgat gagtcggccg acgacgagga
     1621 acggcgcgtt gccgtacgag aacgcagtcc cggcgcggaa ctcacgcggc gagaccgcga
     1681 gggtgcgcac gcaggcggcg agcgcgccac cgtcctgaca cgggttgtcg tgcacgcccg
     1741 aggtgtgatc gagcagcatg cgaggcgtga tcggcggatc ttgcgccgcg aactcgggca
     1801 gccaacgcgc gatgttgtcg tcgagcgcaa tggcaccacg atcgacgaag gtcatgaacg
     1861 tcgccgcggt gagccacttc gtcgacgatc cgaccgacaa cggcgtcgcg cccgacacgc
     1921 tgcctacggc gtgctcgtgg atcaggtcac cgtcgaagtc gacgacgcgc acgatgccac
     1981 cgctcagccc cgccgtccgg acccgctccg cgagcagcgc gtcggtcgcg tcgaaggtcc
     2041 ccggcgcgaa gaccttccgc acgggagggc tcaccatggt ggtcgtcggc ggcgcagccg
     2101 tggtgggagg cgcagttgcg gacgctgcga cggacgaggc tgccgtgctc gcactcgtcg
     2161 agcccgacga gccgcagccg ga
//
LOCUS       DASUGA010000134         2078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1786927, whole genome shotgun sequence.
ACCESSION   DASUGA010000134 DASUGA010000000
VERSION     DASUGA010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1786927"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2078)
                     /locus_tag="VFX21_02605"
     CDS             complement(<1..>2078)
                     /locus_tag="VFX21_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094871.1"
                     /translation="PEHLVTLAEILALVNDPGTTTPTSGPDLLWNLPLEKLDPEGVIL
                     EHVSIVSLLLGQTPLANIVGPNWCAIAGPTICLAGQEQDFLNSATLLDLDFLEVDPSA
                     IDFASIPLSNITPAPDSPLALELAAHPAPGTTSLGSYAIALLARADFPWEELPLGPGG
                     FDITPFARGGRVTYTVQFGAGGLQAQSVTLTITLPDNAVYIPGTAVCTNATGAACTSP
                     LPDPQVSGKTLTFSGVFAIPGETARIAVDAAPLGLVNGPGQLGAASASAVIGETTLNA
                     NAAAGTGVTDPWAGTDIHDPNNAPKVVPDVLYFGSLDAGERDFFTINPGSLPAGAQLT
                     VKMSPIGADADDADLLLVGPPSTGFVGRSGFVGRSGFVGRSGDALDRNAVVDDPIDET
                     NAELAPETLRDLPVDVPPDQVQDISTRRGDANEEVNTTSVGGTYTVQVSGFNDSATDY
                     VLRVAVVAPTEPIECPAIPDGSAASVNIAGRFSGTGSGTSPDTLILYNKSRFTAKAGG
                     DVTPQLQALANATRGWVLPVTTNVEWNDPALDDQCVAAAANAVTRDIHDALLPLYDTA
                     TPPQHVVVIGTDDVLPMRRVPDLTGLYDERDYAPHVGGNDAIARSLATRWFLSDDVYG
                     DPDPATFLGRQVFVPDAAVGRLVETAGDVNAQIQLFLDSQGILDPQSALTTGYDFLAD
                     GAAQVADAVG"
BASE COUNT          318 a          686 c          752 g          322 t
ORIGIN      
        1 cgccgaccgc gtccgcgacc tgagccgcgc cgtctgcgag gaagtcgtag cccgtcgtca
       61 acgcggactg cgggtcgagg atgccctgcg agtcgaggaa gagctggatc tgcgcgttca
      121 cgtcgcccgc ggtctcgacg aggcgaccca cggccgcgtc cggtacgaac acctgacggc
      181 cgaggaacgt tgccgggtcg ggatcgccat acacgtcgtc gctgaggaac caacgggtgg
      241 cgagcgagcg cgcgatcgcg tcgttgccac cgacgtgcgg cgcatagtcg cgctcgtcgt
      301 acaaacccgt gaggtcgggc actcggcgca tcggcaggac gtcgtcggtc ccgatcacca
      361 cgacgtgctg cggtggtgtc gcggtgtcgt acaacggcaa cagcgcgtcg tggatgtcgc
      421 gggtcaccgc gttcgcggcg gccgcaacgc actggtcgtc cagcgcggga tcgttccact
      481 cgacgttcgt cgtgaccggc aacacccagc cccgcgtggc gttggcgagc gcctgcaact
      541 gcggcgtgac gtcaccgccg gccttggcgg tgaaacgcga cttgttgtag aggatcagcg
      601 tgtcgggcga cgtcccgctc ccggtgcccg agaatcggcc ggcgatgttc accgacgccg
      661 ccgatccgtc ggggatggcc gggcactcga tgggctccgt cggcgcgacg accgcgacgc
      721 gcaggacgta gtcggtcgcg gaatcgttga agccgctgac ctgcaccgtg tacgtgccgc
      781 cgaccgaggt cgtgttgacc tcttcgttgg cgtcgccgcg ccgcgtcgag atgtcttgca
      841 cctggtcggg cggaacgtcg accggcaagt cgcgcagcgt ctccggcgcg agctcggcgt
      901 tcgtctcgtc gatcgggtcg tcgacgacgg cgttgcggtc gagcgcgtcg ccgctgcgac
      961 cgacgaaccc gctgcgcccg acgaaaccgc tgcgaccgac gaaaccggtc gacggcgggc
     1021 cgacgagcaa caggtcggcg tcgtccgcgt ccgcgccgat gggcgacatc ttcacggtga
     1081 gctgcgcgcc ggccggcagc gaacccgggt tgatcgtgaa gaagtcgcgc tcacccgcgt
     1141 cgagcgagcc gaagtacaag acgtcgggaa cgaccttggg cgcgttgttc gggtcgtgga
     1201 tgtcggtgcc ggcccacggg tcggtgacac cggtcccggc ggccgcgttc gcgttgagtg
     1261 tcgtctcacc gatcacggcc gacgcgctcg cggcgccgag ctgacccggg ccgttcacca
     1321 gtccgagcgg cgcggcgtcg accgcgatgc gcgcggtctc accggggatg gcgaacacgc
     1381 ccgagaacgt gagcgtcttg ccgctcactt gcgggtccgg cagcggtgac gtgcacgcgg
     1441 caccggttgc attcgtgcac acagcagtgc ccgggatgta caccgcgttg tccggcaacg
     1501 taatggtcaa cgtgaccgat tgcgcctgca agccaccggc accgaactgc acggtgtacg
     1561 tgacccggcc gccgcgtgcg aacggcgtga tgtcgaaccc accgggtccg agcggcagct
     1621 cttcccacgg gaagtcggcg cgggcgagca atgcgatcgc gtacgagccg aggctcgtcg
     1681 tgccgggcgc ggggtgcgcg gcgagctcga gcgcgagcgg cgaatccggc gcgggcgtga
     1741 tgttcgacaa cgggatcgac gcaaagtcga ttgcgctcgg gtcgacttcg aggaagtcga
     1801 ggtcgagcaa cgtcgcgctg ttcaagaagt cttgctcctg ccccgcgaga cagatggtcg
     1861 gacccgcaat cgcgcaccag ttcggaccga cgatgttcgc gagcggcgtc tgcccgagca
     1921 ggagtgacac gatgctcacg tgctcgagga tcacaccctc cgggtcgagc ttctcgagcg
     1981 gcaggttcca caacaggtcg ggaccgctcg tgggtgtggt cgtacccggg tcgttcacga
     2041 gcgcgaggat ctcggccagc gtgacgaggt gctccggt
//
LOCUS       DASUGA010000135         2068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_325386, whole genome shotgun sequence.
ACCESSION   DASUGA010000135 DASUGA010000000
VERSION     DASUGA010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325386"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFX21_02610"
     CDS             <1..216
                     /locus_tag="VFX21_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5094872.1"
                     /translation="ARSSARVAIEYEGGPLRVSVCDSSPAPPRLRDYGPNAVTGRGLM
                     LVDRIAQRWGVEAQDAGKCVWFEVAAG"
     gene            complement(229..561)
                     /locus_tag="VFX21_02615"
     CDS             complement(229..561)
                     /locus_tag="VFX21_02615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5094873.1"
                     /translation="MAGEDAPAIALDVERDGDSAIITVGGELEFGTAAALRSMLIELS
                     QQGADPVVVDIAGLEFIDSTGLSLLVQAKQRFESQGRQFVLRRPTHRVVRVLETSGLA
                     ELFVIQAD"
     gene            624..>2068
                     /locus_tag="VFX21_02620"
     CDS             624..>2068
                     /locus_tag="VFX21_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005261851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX5094874.1"
                     /translation="MTLDSFGARARLTVADSTYDIHRLDAVVSDPRSLPYSLRVVLEN
                     LLRREDGGNVTGDDIAALADRSRASTEAGTRELQFMPARVLMQDFTGVPAIVDLAAMR
                     DAMVALGGDPSAVEPGIPVDLVIDHSIIADVAGIPDAFRKNAALEFERNRERYTFLRW
                     AQGAFRTLRVFPPNRGICHQVNLEYLAPVVFRADGVAYPDTLVGTDSHTPMVNGLGVL
                     GWGVGGIEAEAAMLGQPLSLLQPLVVGIELVGALPAGSTATDLVLTVAELLRQHGVVG
                     KFVEFYGDGVARVPLENRATIGNMSPEYGSTCTIFPIDDETLRYLRATNRPPDLIALV
                     EAYAKEQGLWHDPAARPVYDETMTLDLSSVEPSLAGPARPQDRVPLSDAKAGFTRALL
                     AFRHESGDTPIGVAPASTDESVLESFPASDPPAPAADAEANELPPVGSTRRPISVDLK
                     HCPVRLADGREFELYDGHVVIAAITSCTNTS"
BASE COUNT          315 a          704 c          700 g          349 t
ORIGIN      
        1 gcccgttcct cggctcgcgt tgcgatcgaa tacgagggcg gcccgttgcg cgtctcggtg
       61 tgcgacagca gcccggctcc accgcggttg cgcgactacg gccccaacgc ggtgaccggc
      121 cgagggctga tgctcgtcga ccgcatcgcc cagcggtggg gagtcgaagc ccaggacgcc
      181 ggaaaatgcg tctggttcga ggtcgcggcc ggctgaccgc cgctcgcgtc agtcggcctg
      241 gatcacgaac agttcggcga gtcccgacgt ctcgagcacc cgaaccactc gatgggtcgg
      301 ccggcgcagc acgaactgcc ggccctgcga ttcgaagcgc tgcttcgcct gcaccagcag
      361 gctgaggccg gtcgagtcga tgaactccaa gccggcgatg tcgaccacga ccggatccgc
      421 gccctgctgc gagagctcga tcagcatcga ccgcagtgcg gccgcggtgc cgaactcgag
      481 ctcgcccccg acggtgatga tggcgctgtc gccatctcgc tcgacatcga gcgcgatggc
      541 cggcgcgtcc tcccctgcca cagcggagga gcgtacctgc ccgcggggac tccggcatcg
      601 cggttcaggg atagcgtcag gctgtgacgc tcgacagctt cggtgcccgg gctcggctca
      661 cggttgccga ctcgacgtac gacattcacc ggctcgatgc cgtcgtgtct gacccgcggt
      721 cgctgcccta ctcgttgcgg gtcgtgctcg aaaatctgct gcggcgggag gacggcggca
      781 acgtcaccgg cgacgacatc gccgcgctgg ccgaccgttc gcgcgcgtcg accgaagccg
      841 ggacccgcga actccagttc atgccggcgc gcgtgctcat gcaggacttc acgggcgtgc
      901 cggcgatcgt cgacctcgcg gcgatgcgcg atgcgatggt cgccctcggt ggcgatccca
      961 gcgcggtcga gccgggaatt cccgtcgatc tggtgatcga ccattcgatc atcgccgacg
     1021 tcgcgggcat acctgacgcg ttccgcaaga acgcggcgtt ggagttcgag cgcaaccgcg
     1081 agcggtacac gttcctgcga tgggcgcaag gcgcgttccg aacgttgcgt gtgttcccgc
     1141 cgaaccgcgg catctgtcat caagtgaatc ttgaatacct cgcgccggtc gtgttccggg
     1201 ctgacggcgt tgcgtatccc gacacactgg tcggcaccga ttcgcacaca ccgatggtga
     1261 acggtctcgg cgtgctcggc tggggtgtcg gcggcatcga agccgaggcc gccatgctcg
     1321 gccagccgtt gtcgttgctg caaccgctgg tcgtcggtat cgagttggtc ggcgcgctgc
     1381 ccgcgggctc gacggcaacc gacttggtgt tgacggtggc cgagttgctg cgtcaacacg
     1441 gcgtcgtcgg aaagttcgtg gagttctacg gcgatggcgt cgctcgcgtg ccgctcgaaa
     1501 atcgcgccac gatcggcaac atgtcgccgg agtacggctc gacctgcacg atctttccca
     1561 tcgacgacga aacgctgcgc tatttgcgcg cgacgaaccg gcccccggat ctgatcgcgt
     1621 tggtcgaggc gtacgccaag gaacagggct tgtggcacga ccccgccgcc cggcccgtgt
     1681 acgacgaaac gatgacgctc gatctctcgt cggttgagcc gtcgctcgcg ggtcccgcgc
     1741 gtccgcaaga ccgcgtgcca ttgtcggacg cgaaggcagg gttcacgcgc gcgttgctcg
     1801 cgttccggca cgaatctggt gacacgccca ttggcgtcgc acccgcgagc accgacgaat
     1861 cggtgctcga gtcgttcccg gcgagcgacc cgcccgcgcc cgccgccgac gcagaagcga
     1921 acgagttgcc gccggtcggg tcgacgcgcc ggccgatctc ggtcgacctc aagcattgtc
     1981 ccgtgcggtt ggccgacggc cgcgagttcg agttgtacga cggccacgtc gtgatagctg
     2041 cgatcacgtc gtgcacgaac acgtcgaa
//
LOCUS       DASUGA010000136         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_777295, whole genome shotgun sequence.
ACCESSION   DASUGA010000136 DASUGA010000000
VERSION     DASUGA010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777295"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..625)
                     /locus_tag="VFX21_02625"
     CDS             complement(167..625)
                     /locus_tag="VFX21_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5094875.1"
                     /translation="MFVTVESGPDRQAWVSLLELADEPVPLARYLDDGTLYGLTGDGG
                     PRAAVLVIMLDDARAELRAVAVAETEQGKGLGTEIVERVFGALRERGVREVVVGTATS
                     GVRQLAFYQRLGFRLTHIERDFFTVDNGYPADLSENGIPTRDMVWMSMQL"
     gene            650..1765
                     /locus_tag="VFX21_02630"
     CDS             650..1765
                     /locus_tag="VFX21_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5094876.1"
                     /translation="MRYRRMPIEIESPEQLGYDTITNNLSESSVADRRLADLGLDLDL
                     DALLLCYGDHLGDPVLREAVAAGGENLSADDVLVTPGAAAALFASATALLDPGDHIVV
                     VRTNYATNLETPRAIGAELDIVDLQYDEQWQLDPERIAERVQPGVTKLISVTCPHNPT
                     GTTFSLNTLCTLVDIAERADAVLLVDETYRDLTHEVVLPMAATLSPRAISVSSMSKAY
                     GLPGLRAGWAVCRDSQLAETLLAAKEQMVICGPTIDEAIAGAVLSARERILPPILADV
                     RVRLAFVRDWMRGQDTFEWIEPEGGVVGLVRFKPGVEVDTDRFYERLLRDYGTYVGPG
                     HWFELDDRHFRLGFGWPGEKELQTGLVALTEAAAISG"
     gene            complement(1752..>2383)
                     /locus_tag="VFX21_02635"
     CDS             complement(1752..>2383)
                     /locus_tag="VFX21_02635"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEX5094877.1"
                     /translation="ETDVFGSLQYFGARQPDSDRFRLWEVAGAAHADTYFFSASPFDS
                     GSLTVAELAALVAKSENTGLPVKLPINSGPQMHYVLQRAFDALERWVRDGEGPASAPR
                     LDADDNGTMETDGLGIARGGVRTPWVDAPLEVLSGLGQPGSMMELFGITRPLPDGARA
                     ERYPRGRDEFVPRFDAATQDAVDAGFLLEADANEIRALGRAGWERVNPK"
BASE COUNT          392 a          801 c          801 g          389 t
ORIGIN      
        1 cgaggacgcg cgcgcggcgg gggcgacgat tctccgggaa cccgcgctgt gcttcttcgg
       61 cgggtggcag ggctacttcg aggatccctg cggtttcgtg tgggaggtcg cgcacaaccc
      121 gggctggtca gtcgacgccg acggcaccgt gcacctcggc cccccgtcat aactgcatcg
      181 acatccagac catgtcgcga gtggggatgc cgttctcgga caagtccgcc ggatacccgt
      241 tgtcgacggt gaagaagtcg cgctcgatgt gcgtgagccg gaatccgagg cgctggtaga
      301 acgcgagctg gcgcacgccg ctggtcgccg ttccgacgac cacctcccgc acgccacgtt
      361 cgcgcagcgc gccgaaaacc cgctcgacga tctcggtccc caagcccttg ccctgttcag
      421 tttctgcgac ggcaacggct cgcagctcgg cgcgcgcatc gtcgagcatg atcacgagca
      481 cggccgcgcg cggcccgccg tctccggtga gcccgtagag cgtgccgtcg tcgagatacc
      541 gggcgagcgg caccggctcg tcggcgagtt ccagcagcga aacccaggcc tgccgatccg
      601 gacccgattc gaccgtgacg aacacggttg ctgaccgtag tgtcggggcg tgcgttaccg
      661 ccgtatgccg atcgagatcg agtcaccgga gcagctcggt tacgacacga tcacgaacaa
      721 cctgtcggag agttccgtcg ctgaccgccg actcgccgat ctcggcctcg acctcgacct
      781 ggacgcgctg ctcctctgtt acggcgatca tctcggcgac cccgtgctgc gcgaagcggt
      841 ggcggccggc ggcgagaatc tttcggccga cgatgtcttg gtgacgccgg gcgcggcggc
      901 cgcgctgttc gcgagcgcga ctgcactgct cgatcccggc gatcacatcg tggtcgtgcg
      961 caccaactac gcgacgaact tggagacgcc gcgcgcgatc ggtgccgaac tcgacatcgt
     1021 cgacttacag tacgacgaac agtggcagct cgatcccgaa cggatcgccg agcgcgtgca
     1081 gcccggtgtc acgaagctga tctcggtgac gtgtccgcac aacccgacgg gcacgacgtt
     1141 ttcgctcaat acgctatgca cgctcgtcga catcgcggag cgcgccgatg cggtgttgct
     1201 cgtcgatgag acgtaccgcg atctgacgca tgaagttgtg ttgccgatgg cggcaaccct
     1261 gtcgccgcga gcgatcagcg tgtcttcgat gtcgaaggcc tacggactcc cgggtctgcg
     1321 cgcgggctgg gccgtgtgcc gcgattcgca actggccgag acgttgctcg ccgccaaaga
     1381 acagatggtc atctgcggac cgacgatcga cgaggcgatt gcgggcgcgg tgctcagcgc
     1441 ccgcgaacgc atcctgccgc cgatcctcgc cgacgtgcgc gtgcggctcg ccttcgtgcg
     1501 cgactggatg cgcggtcagg acacgttcga gtggatcgaa cccgagggcg gcgtcgtcgg
     1561 gttggtgcgt ttcaagcccg gcgtcgaggt cgacaccgat cggttctacg aacgcctgct
     1621 gcgcgactac ggcacctacg tcgggccggg acactggttc gagctcgacg accggcactt
     1681 ccggcttggg ttcgggtggc cgggcgagaa agaactgcag acgggcttgg tcgcgctgac
     1741 cgaagcggcg gctatttcgg gttgacgcgc tcccaccccg cgcgcccgag cgcgcggatt
     1801 tcgtttgcgt ctgcttcgag caagaagccg gcgtcgactg cgtcctgggt cgcggcgtcg
     1861 aagcgaggaa caaactcgtc gcggccgcgc ggataacgct ccgcccgcgc gccgtcgggc
     1921 agcgggcgcg taatgccgaa caactccatc atcgagccgg gctgaccgag accggaaagg
     1981 acttcgagcg gcgcatcgac ccacggcgtg cgaacgccgc cgcgcgcgat gccgagccca
     2041 tcggtttcca tcgtgccgtt gtcgtcagca tcgagccgcg gtgccgacgc gggaccctcc
     2101 ccatcgcgca cccagcgctc gagtgcgtcg aacgcgcgct gcagcacgta gtgcatctgc
     2161 ggcccggaat tgatcggcag cttgaccggt aaacccgtgt tctcggactt cgccacgagg
     2221 gccgccagtt cggcgacggt gagcgaaccg gaatcgaacg gcgatgccga aaagaagtac
     2281 gtgtccgcat gagccgctcc cgcaacctcc cagagccgga accggtcgct gtcaggttgg
     2341 cgcgcaccga aatactggag cgagccgaac acatcggttt cgc
//
LOCUS       DASUGA010000137         8270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1852542, whole genome shotgun sequence.
ACCESSION   DASUGA010000137 DASUGA010000000
VERSION     DASUGA010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8270
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1852542"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1974
                     /locus_tag="VFX21_02640"
     CDS             <1..1974
                     /locus_tag="VFX21_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5094878.1"
                     /translation="SASLVLLAEATGQLVYANQRVDALGYATDEVVGKNVVDFLHPDD
                     RDADVSAFVRAVDNAQDREPDEIRVRRADGTYALYEFLVTNLLDDEDVCGMIIEGRDL
                     GDRYEQQRRYRLLFEQSPVAQVLVPPDRPSMIANEAFARLFGYNRVSLLDTHFSVIFG
                     SSDAHAAFIDELLDGPSHELAVTMTLRRADDEAFAAHVTATAVYDQEGALDHVLASVE
                     DITEAVRQAAELEASEQRLRAIIDNSPDIIGVVHEGGLFEQSGRGTGRLGYPGGFVPP
                     GGFRALLHEDDVPTAIQALAEVWQGTRDHQSPLEVRLRNAAGEYETFELVGQVTGGGA
                     LGRSVVVTARNINERRKAKDLFYAAAERLRVTFEHAPLGVSLVSMEGRILDINASACH
                     MSGKTRDELVGTEAQDVVHPDDRDLAIEYTTRQLAGEEVAAEFRMLRPDGSIVWALSE
                     ARLYEPPTGDPFIITMQTDITERKRLESELAIQATRDPLTGLLNRGAFVTQLELELAR
                     RDPPSFALLFIDLDKFKPVNDKLGHSAGDAVLAQVADRLEKHTRRGDVVARLGGDEFV
                     VIYHGRDIKKGVAHAAERLCAAVAEPIVIHGQRVSVGASVGAAIARPGEGAAELMQRA
                     DEASYLAKQSGGSCVVYADPVGVSATARRRSSA"
     gene            complement(1952..2707)
                     /locus_tag="VFX21_02645"
     CDS             complement(1952..2707)
                     /locus_tag="VFX21_02645"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX5094879.1"
                     /translation="MSWTQLIAAIVAALVVTQFATAATTVYLHRALTHRAMTVRPEAA
                     LPFRFVIWITTGMKPREWVAVHRKHHAATDTVDDPHSPKIVGFWRVQLGNVGLYKKVA
                     ADEINTRKYARDIRTDRLDAMFFDHALFGLGVGIAILVTVMWGLGFGLLVGFLAAGIH
                     MVTYIMMSGAINAVGHTYGKRPYDNSATNGQLLALVSGGEGLHNNHHAAPTSARFSLH
                     KGEIDPGWYLVKALVWMRLAQVRHEDVKLKNAA"
     gene            2806..3420
                     /locus_tag="VFX21_02650"
     CDS             2806..3420
                     /locus_tag="VFX21_02650"
                     /inference="COORDINATES: protein motif:HMM:NF037007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5094880.1"
                     /translation="MALPAETPTTTKEQILVVARRRFAEHGYAGTSLTEIADEVGIRR
                     PSLLYHFPSKETLYHDVLLEAFGDWSSLVAEATVGTREGWPQVERMLRAAFTFFEERP
                     DFVRLVRWEALEGGPLLQGQLAMLLQPLFDRGAAFLEREMDAGRLRRYDARQLLVTGY
                     GAVLSYLSDAPLMTGLLDTDPLSPEALAARREHIIDVLKNAVAV"
     gene            complement(3423..4157)
                     /locus_tag="VFX21_02655"
     CDS             complement(3423..4157)
                     /locus_tag="VFX21_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEX5094881.1"
                     /translation="MIDHQQCVAAIRRDGELLARAAETAGDDAPMPSCPDWTIADLLS
                     HIGRIHRYVTAIVTARPNPPAEHWSRMTSPPVGERLAWFRAGYPALADALGAADPDDA
                     VWTWATDPRVRFWSRRMAHEIAVHRWDAQRATGAPPDPIQTEFAADAIQEAFDILPAR
                     KLGDRLTGTGETIHLHCTDTDGEWLLRLTPGGLDVTREHAKGDVAARGAASDLLLLVW
                     GRIEPDAVEVFGDASLLTRWQELARF"
     gene            complement(4154..4861)
                     /locus_tag="VFX21_02660"
     CDS             complement(4154..4861)
                     /locus_tag="VFX21_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX5094882.1"
                     /translation="MCGRYVQVSSPTILAERFQVETVRVTEKEPDYNVTPRADVPVVA
                     VSRGERTLDLVRWGLVPSWAKDLSIGDKLINARADTVASKPAYKRAFVKRRCIVPADG
                     FYEWKELAAPAGTKKKVKQPYFIRRRDGEPLAFAGLWEIWHDPNVGDDAPRIRSCVII
                     TTDANDVLAPIHNRMPVVLPESEWGRWLDPEFNDVDALQKLLVPAPSDEFEAWEVTTL
                     VNKPANNGPELLERAHT"
     gene            5054..5677
                     /locus_tag="VFX21_02665"
     CDS             5054..5677
                     /locus_tag="VFX21_02665"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5094883.1"
                     /translation="MSRTLTKGAQTRQRIVELAAPVFNKFGYVGASMRDLVDATGLEK
                     GGIYNHFGSKEQLALEAYDHAMNLVENSLARSQHDEDDAIERLQAMIRTFARGYRNPV
                     VAGGCPIMNTAIEADDTQPDLRDRARASMNRWHRMIGRIVKDGKAAGTVRADVDPYDV
                     AALCTAALEGGLMLSRLFEDASYIDRVVEQLVSQVADLRVSPQEETA"
     gene            5674..6210
                     /locus_tag="VFX21_02670"
     CDS             5674..6210
                     /locus_tag="VFX21_02670"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX5094884.1"
                     /translation="MTALPTSSPSQEEGVRSRVVSWTDPARARFALRGRDGLEQLKAI
                     ARGDAAPPPAAALLGMELATVERGRVVFDIVADELHENPMGTMHGGIVATLVDSAMGC
                     AVLSMLPAGTSYTTLELKTNYVRPIVQTTGRIHAEGRVVHLGGRVATTDAQVIDDAGT
                     LYAHATSTSLVMRPGAVR"
     gene            6207..6713
                     /locus_tag="VFX21_02675"
     CDS             6207..6713
                     /locus_tag="VFX21_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5094885.1"
                     /translation="MTVDRLRLADGAVLTVRPIEPEDVERLHRMFFRLSPTTIYKRFF
                     SPIQHPSRALLERLASVDHDCREALVALDGDEIVAVARYDSECGSHSAEIAVTVEDDW
                     QHRGVGRKLARRLALVAMDHGFDEFSAIVLPDNRDALGLLRRLSPDAHIEFDSSGFSA
                     TLPLRRAS"
     gene            complement(6710..7168)
                     /locus_tag="VFX21_02680"
     CDS             complement(6710..7168)
                     /locus_tag="VFX21_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HEX5094886.1"
                     /translation="MAGYSGTPLPQKLGIKAGAKVALVRAPSGFEKELQPLPDNVLLR
                     EQARGAQDVVLFFATRRAELERRFATLARSVSPAGGLWIAWPKRTACVATDLSENIVR
                     DVGLEHGLVDNKVCAVDDTWSGLRFVYRLKDRPAAKKAKKKTAKPAKKSR"
     gene            complement(7215..>8270)
                     /locus_tag="VFX21_02685"
     CDS             complement(7215..>8270)
                     /locus_tag="VFX21_02685"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5094887.1"
                     /translation="MVVASLGMGPVIDRHDRRLILLGAQSGLAVASAILLGGALMGSP
                     PLALVFGAAALNAGLQSVAMPTRSAMTPNLVPIETLPSATALNQVMWNTAMVVGPALG
                     GVIVATAGLEWAYGIDLCTYAVAIVFVWLLRSQVPERDPDAEEERGWSAVLAGFRYLR
                     GKRVVQSTFTVDIVAMVFGMPRALFPILAREQFGGGPEIVGYLFAAPAFGALLGALGS
                     GWVSRIQRQGLAILVAVTLWGAGIVGFGLSGDMLGPALVFLAIAGAADVISAVFRSTI
                     QLTTTPDALRGRLSAFNILVVAGGPRLGDFEAGQVAALFSPTASVVSGGLFCIGGVAI
                     IATLVPRFARWRVGDPP"
BASE COUNT         1357 a         2849 c         2780 g         1284 t
ORIGIN      
        1 tccgcgtcat tggtgttgtt ggcggaggcc accggtcagc tcgtctacgc caaccagcgt
       61 gtcgacgccc tcggctacgc caccgacgag gtcgtgggca agaacgtcgt cgacttcctg
      121 caccccgacg accgcgacgc ggacgtgagc gcgttcgtgc gggccgtcga caacgcgcaa
      181 gatcgtgagc ccgacgagat ccgcgtccga cgcgcggacg gcacgtatgc actgtacgag
      241 ttcctggtca cgaacttgct cgacgacgaa gacgtgtgcg gcatgatcat cgagggtcgt
      301 gacctcggcg atcgatacga acaacaacgc cgctaccgcc tgttgttcga gcagtcgccg
      361 gttgcgcaag tgctcgtgcc acccgatcgg ccatcgatga tcgccaacga agcgttcgcg
      421 cggctgttcg gatacaaccg ggtctcgctg ctcgacacgc acttctctgt catcttcggg
      481 agctccgatg cgcacgccgc gttcatcgac gagctgctcg acggtccgtc gcacgaactg
      541 gcggtcacga tgacgctgcg ccgggcggac gacgaggcgt tcgccgcgca cgtgacggcc
      601 accgcggtgt acgaccaaga aggcgcgctc gaccacgttc ttgcgagcgt cgaagacatc
      661 accgaagcgg tgcggcaggc agctgagctc gaagcgagcg agcagcgtct gcgcgccatc
      721 atcgacaact cgcccgacat catcggcgtc gtccacgaag gtggcctgtt cgagcagagc
      781 gggcggggaa ccggccgcct cggctatccg ggtgggttcg ttccgcccgg cggcttccgg
      841 gcgctcttgc acgaggacga tgttccgacg gccatacaag cgctcgcgga agtctggcaa
      901 ggcacgcgcg atcatcagtc gcccctggag gtgcgactcc gcaatgccgc cggtgagtac
      961 gagacgttcg agctcgtcgg gcaagtcacg ggtggcggcg cgctcggccg ttcggtggtc
     1021 gtcaccgcgc gcaacatcaa cgagcgccgc aaggccaagg atctcttcta cgcggctgca
     1081 gaacgtctgc gcgtcacctt cgagcatgcg ccgctcggtg tttcgctcgt gtcgatggaa
     1141 gggcgcatcc tcgacatcaa cgcgtcggcg tgccacatgt ccggcaagac tcgcgacgag
     1201 ctcgtgggca cagaggcgca ggatgtggtg catcccgacg acagagatct ggccatcgag
     1261 tacacgacgc ggcagttggc cggcgaagag gttgcagccg agttccgcat gctgcgaccc
     1321 gacggatcga tcgtgtgggc attgtcggaa gcccggctgt acgagccgcc gaccggtgac
     1381 ccgttcatca tcacgatgca gaccgacatc acggagcgca agcgcctgga gtcagagctc
     1441 gcgatccagg cgacacgcga tccgttgacg ggactactca accgcggcgc cttcgtcacc
     1501 caactcgagc tcgagctggc gcggcgcgat ccgccgagct tcgcgctcct gttcatcgac
     1561 ctcgacaagt tcaagcccgt gaacgacaag ctcgggcaca gcgcgggcga cgccgtgctg
     1621 gcgcaggtcg ccgatcgcct cgagaaacac acgcggcgcg gtgacgtggt cgcccgcttg
     1681 ggcggggacg agttcgtcgt gatctatcac ggtcgagaca tcaagaaggg cgtcgcgcac
     1741 gcggccgagc ggctgtgcgc cgccgtcgcc gaaccgatcg tcattcacgg ccaacgggtc
     1801 tcggtcggcg cgagcgtcgg cgctgcgatc gcccggcccg gcgaaggcgc ggccgagctc
     1861 atgcaacgcg ccgacgaggc gtcctatctc gcgaagcagt cgggcggttc gtgcgtcgtg
     1921 tacgccgacc cggtcggcgt gagtgcgacg gctaggcggc gttcttcagc ttgacgtctt
     1981 cgtgacgcac ctgcgcgagg cgcatccaca cgagcgcctt gacgagatac cagccgggat
     2041 cgatctcgcc cttgtgcaac gagaagcgcg ccgacgtcgg ggctgcgtgg tggttgttgt
     2101 gcagcccttc gccgccgctc accaacgcga gcagttgccc gttggtcgcg gagttgtcgt
     2161 acggccgttt gccgtaggtg tgcccgaccg cattgatcgc gcccgacatc atgatgtagg
     2221 tgaccatgtg gatgccggcg gcgaggaagc cgacgagcag cccgaatccg aggccccaca
     2281 tgacggtgac gaggatcgcg atgccgacgc cgaggccgaa caacgcgtgg tcgaagaaca
     2341 tcgcgtcgag gcggtcggtg cggatgtcac gcgcgtactt gcgggtgttg atctcgtcgg
     2401 ccgcgacctt cttgtagagg ccgacgttgc cgagctggac gcgccagaac ccgacgatct
     2461 tcgggctgtg cgggtcgtcg acggtgtcgg tggccgcgtg gtgcttgcgg tgcacggcga
     2521 cccactcccg aggcttcatc ccggtggtga tccagatgac gaaccggaac ggcagcgccg
     2581 cctcgggccg aacggtcatc gcccggtgcg tgagggcgcg gtgcaggtac acggtggtgg
     2641 ccgcggtcgc gaactgggtc acgacgagcg cggccacgat cgcggcgatg agctgagtcc
     2701 acgacatcgg gacgcgagct tactacctac tagtaggtag gctacgggtc aggtgcggaa
     2761 tcccgcaccg ggacgacccg cgatggacgt agcgttcgga gcgacatggc actgcccgcc
     2821 gagaccccga cgaccaccaa ggagcagatc ctggtcgtcg cgcggcgccg cttcgccgag
     2881 cacgggtacg cgggcaccag cctgaccgag atcgcggacg aggtggggat ccgccggccg
     2941 agcctgctgt accacttccc ctcgaaggag acgctctatc acgacgtctt gctggaggcg
     3001 ttcggcgact ggagcagcct cgtcgcggag gcgaccgtcg gcactcgcga gggctggccg
     3061 caggtcgaac ggatgttgcg ggcggcgttc acgttcttcg aggagcgccc cgatttcgtg
     3121 cggctcgtgc ggtgggaggc gctcgaaggc ggtccgctcc tgcaagggca actcgcgatg
     3181 ctgttgcagc ccttgttcga ccgcggcgcc gcgttcttgg agcgcgagat ggacgccggc
     3241 cggttgcggc gctacgacgc gcgccagctg ttggtgacgg gctacggcgc ggtgttgtcg
     3301 tacctttcgg acgcgccgtt gatgacgggc ttgctcgaca ccgacccact gtcgcccgaa
     3361 gcactggccg cccgccggga gcacatcatc gacgtgctca agaacgcggt agctgtgtag
     3421 ttctagaaac gcgcgagttc ttgccagcgc gtcaggagtg acgcgtcgcc gaagacttcg
     3481 accgcgtcgg gttcgatgcg gccccatacc aacaacagca agtcgctggc ggcgccgcgg
     3541 gcggcgacgt cgcccttggc gtgttcgcgc gtgacgtcga gcccgcccgg cgtcaggcgc
     3601 agcagccact cgccgtcggt gtcggtgcag tgcaagtgga tcgtctcgcc ggtgccggtg
     3661 agccggtcgc cgagcttgcg cgcgggcagg atgtcgaacg cttcctggat cgcgtcggcc
     3721 gcaaactccg tctggatcgg atcgggcggc gcgccggtgg cacgctgcgc gtcccagcgg
     3781 tgcaccgcga tctcgtgcgc catgcgtcgc gaccagaacc gcaccctcgg gtccgtcgcc
     3841 cacgtccata ccgcgtcgtc gggatccgcg gcgccgagcg cgtcggccag cgccgggtat
     3901 ccggcacgga accacgcgag ccgttcgccg acgggcggtg acgtcatccg gctccagtgc
     3961 tccgccggcg ggttcggccg cgccgtcacg atcgcggtga cgtagcggtg gatgcggccg
     4021 atgtgcgaga gcaggtcggc gatcgtccaa tcggggcacg acggcatcgg cgcgtcgtcg
     4081 cccgcggtct ccgccgcgcg cgccagcagc tcaccgtcgc ggcggatcgc cgcgacgcac
     4141 tgctgatgat cgatcatgtg tgcgctcgct cgagcagttc cggaccgttg ttcgccggct
     4201 tgttcacgag agtggtgacc tcccacgctt cgaactcgtc actcggcgcg ggcacgagca
     4261 acttctgcaa cgcgtcgacg tcattgaact cgggatcgag ccaacgaccc cactccgact
     4321 cgggcaacac gaccggcatg cggttgtgga ttggtgcgag cacgtcgttc gcgtcggtcg
     4381 tgatgatcac acaactgcgg atgcgcggcg cgtcgtcacc gacgttcggg tcgtgccaga
     4441 tctcccacag ccccgcgaac gcgagcggct caccgtcgcg acggcggatg aagtacggct
     4501 gcttgacctt cttcttggtg cccgccggcg ccgcgagctc cttccactcg tagaagccgt
     4561 ccgcgggaac gatgcagcgc cgcttcacga aggcccgctt gtacgcgggc ttgctcgcga
     4621 ccgtgtcggc gcgggcattg atgagcttgt cgccgatcga gagatccttg gcccacgacg
     4681 gcaccagccc ccagcggacg agatcgagcg tgcgctcccc ccggctgacc gccaccacgg
     4741 gcacgtcggc ccggggcgtg acgttgtagt cgggctcctt ctccgtcacc cgcacggtct
     4801 ccacctggaa ccgctcggcc aggatcgtcg gcgagctgac ctgcacatac cgaccgcaca
     4861 tgaatatgtc cttgtcgctc cgcacgtgct cgcgtgaggc agcccggatt gttggcccgc
     4921 gctccgctcc gcgagtgcct ccgcaagcta cggcaccgcc ctgttggcgc cggcccttag
     4981 atccgccttg ttttcagaac gatcggtctg taatatcgaa ccggacggtc ggatttcaac
     5041 aggagcaagc actgtgagcc gcactctcac caagggcgcc cagactcgcc agcggatcgt
     5101 ggagctggcc gcgcccgtct tcaacaagtt cggctacgtg ggcgcgtcga tgcgcgacct
     5161 cgtcgacgcg accgggctcg agaagggcgg catctacaac cacttcgggt cgaaggaaca
     5221 gctcgcgctg gaggcgtacg accacgcgat gaacctggtc gagaactcgc tcgcccggtc
     5281 gcagcacgac gaggacgacg ccatcgaacg cctccaggcg atgatccgca ccttcgcccg
     5341 cggctatcgc aaccccgtcg tcgccggcgg ctgcccgatc atgaacaccg ctatcgaggc
     5401 cgacgacacg caacccgacc tgcgcgaccg ggcccgggcc tcgatgaacc gctggcaccg
     5461 catgatcggc cgcatcgtca aggacggcaa ggcggccggc accgtgcgcg ccgacgtcga
     5521 cccctacgac gtcgccgcgt tgtgcaccgc cgccctcgaa ggcggcctca tgttgtcccg
     5581 cctgttcgag gacgcgtcgt acatcgaccg cgtcgtcgag caactcgtct cgcaagtcgc
     5641 agacctccgc gtctcccccc aggaggaaac ggcatgactg ctctgcccac cagttctccg
     5701 tcccaagaag aaggcgtgcg ctcacgcgtc gtcagctgga ccgaccccgc ccgggcgcgc
     5761 ttcgcgctac gcggccgcga cgggctcgaa caactcaagg cgatcgcccg aggagacgcc
     5821 gcgcctcccc ccgcggcggc gctcctcggg atggagctcg ccaccgtcga acgcggccgc
     5881 gtcgtgttcg acatcgtggc cgacgaactg cacgagaacc cgatgggcac gatgcacggc
     5941 ggcatcgtgg cgacactcgt cgacagcgcc atgggctgcg ctgtgctctc gatgctgccc
     6001 gccggcacct cgtacacgac gctcgagttg aagacgaact acgtccgccc gatcgtgcag
     6061 acgaccggcc gcatccacgc cgagggccgc gtcgtccacc tcggtggacg cgtcgcgacg
     6121 accgacgcac aggtgatcga cgacgcaggc acgctgtacg cgcacgcgac ctcgacgtct
     6181 ctggtgatgc gacccggagc ggtccggtga ccgtcgaccg cctccggctc gccgacggtg
     6241 cggtgctgac cgtgcggccg atcgagcccg aggacgtcga gcggttgcac cggatgttct
     6301 tccggctgtc gcccaccacc atctacaagc ggttcttctc cccgatccag catccgtcgc
     6361 gggcgctgct cgagcggctg gcctcggtcg accacgactg ccgggaggcc ctggtcgccc
     6421 tggacggcga cgagatcgtg gccgtggccc gctacgacag cgagtgcggc agccactcgg
     6481 ccgagatcgc ggtcaccgtc gaggacgact ggcagcaccg gggtgtaggc cgcaagctcg
     6541 cccgccggct cgcgctcgtc gcgatggacc acggcttcga cgagtttagc gcgatcgtgc
     6601 tccccgacaa ccgcgacgcg ctcggcctgt tgcggcgcct gtcaccggat gcgcacatcg
     6661 agttcgacag cagcggtttc tccgcgacgt tgccgttgcg gcgcgcgagt tagcgcgact
     6721 tcttcgcggg cttcgcggtc ttcttcttcg ccttcttcgc cgcgggccga tccttcagcc
     6781 ggtagacgaa gcgcagcccg gaccatgtgt cgtcgaccgc gcacactttg ttgtcgacga
     6841 gcccgtgttc gagcccgaca tcgcggacga tgttctcgga caggtcggtc gcgacacacg
     6901 cggtgcgctt cggccacgcg atccagagac ctcccgcggg agacaccgaa cgcgcgagcg
     6961 tcgcgaagcg acgctcgagc tcggcgcgtc gtgtcgcgaa gaacaagacg acgtcttggg
     7021 caccacgcgc ttgctcgcgc aggaggacgt tgtcgggcag cggctgcagt tccttctcga
     7081 agccgctcgg tgcgcggacg agcgcgacct tcgcgccggc cttgatcccc agcttctgag
     7141 gaagtggtgt tccggaatac cccgccatca cgcgcagtca atcgcggtac gcgtccgcgg
     7201 tcaatccgaa cgacttacgg tggatcaccg acgcgccagc gcgcgaaacg cgggacaagt
     7261 gtcgcgatga tcgcgacgcc gccaatgcag aacaatccgc cactcacgac cgacgcggtc
     7321 ggcgagaaca acgccgcgac ctggcccgct tcgaagtcac cgaggcgcgg cccacctgcg
     7381 acaacgagga tgttgaacgc cgaaagtcgc cctcgcaacg cgtccggcgt cgtcgtcagt
     7441 tggatcgtcg accggaagac agcgctgatc acgtccgcgg cgcccgcgat cgcgagaaag
     7501 acgagcgccg gcccgagcat gtcgccggac agtccgaagc cgacgatccc cgcgccccac
     7561 agcgtgaccg cgacgaggat cgcaagtcct tgccgctgga tgcgcgacac ccacccggat
     7621 ccgagcgcgc cgaggagcgc gccgaacgcg ggcgcggcga agaggtaacc gacgatctcc
     7681 ggtccgccac cgaattgctc gcgcgcgagg atcggaaaca gtgcgcgcgg catcccgaac
     7741 accatcgcga cgatgtcgac ggtgaacgtc gactgcacga cgcgcttgcc gcgcagataa
     7801 cgaaacccgg cgagcactgc cgaccacccg cgctcctctt cggcatcggg atcgcgctcg
     7861 ggcacctgcg agcgcagcag ccacacgaag acgatcgcga cggcatacgt acacaggtcg
     7921 atgccgtatg cccactcgag tcccgcggtc gcgacgatca cgccgccgag cgcgggcccg
     7981 acgaccatcg cggtgttcca catcacctgg ttgagtgcgg tcgccgacgg cagcgtctcg
     8041 atcggtacga ggttcggtgt catcgccgaa cgcgtgggca tcgcgacgga ttgcaggccg
     8101 gcgttcagcg cggcggcgcc gaagacgagc gcgagcggcg gcgatcccat gagcgcaccg
     8161 ccgagcagga tcgcggacgc cacggccaac cccgactggg cgccgagcag gatcaggcgc
     8221 cggtcgtggc ggtcgatcac cgggcccatg cccagcgacg ccacgaccat
//
LOCUS       DASUGA010000138         2613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1434978, whole genome shotgun sequence.
ACCESSION   DASUGA010000138 DASUGA010000000
VERSION     DASUGA010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434978"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1202)
                     /locus_tag="VFX21_02690"
     CDS             complement(108..1202)
                     /locus_tag="VFX21_02690"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5094888.1"
                     /translation="MLAADLPKISADAHVDEPHDLWYERLPESLREGAPRRIQGNEEG
                     GWTLVVNGDAVGWDDASVTEARALEADRAAAASPRVRFEMMRRDGVNAEVIYPTIGLY
                     MWNVADPAVGDAGCRIYNDWIREQLGGESRIKLAAMLPTWTLELALAEIARVGDDPSI
                     GGLLLPLVGTPEWNLPQWEPLWAALAEIGKPVVMHQGTGHDMIFYRGWGSATANLLTT
                     QSMAARATALLSCSGVLERHPALHVVLVEVNAGWLAWTMSTLDEYYQAHESWSKPKLD
                     ELPSHYIRRQIHATFQTDPVAVHNIPLTGTDGLLWGNDFPHPESTYPHSNKVLDDLFV
                     GVDERDAIAITSGNARTLFGFDDSVLAARP"
     gene            1372..2445
                     /locus_tag="VFX21_02695"
     CDS             1372..2445
                     /locus_tag="VFX21_02695"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5094889.1"
                     /translation="MSHDAVKTHLASKYHGAYDAGLMAFGASMGSTMSVEANSRWRDQ
                     QNLGEPEGPIGMGVNRRHPASGRAGHSDANARLADMDVDGVEASVSYCEVSAFRYLYL
                     IEDGWRESTRAFNLALADFASADPKRLIVNYQIPIHDIDAAVDEVRWAASAGCKSLQL
                     PVYPAELGLPDYWDVRYDPLWNAITDADLPICLHIGLNTALDDLARRDPTPQKGIFVP
                     LVALSAAEPLGMWIMGGVFERHPLLKVVFVEPGLGWVAWWLYIVDDLAARQGYDFPAI
                     TYAPSHYFHRNVFLTFIDEPHAIRMANETLGIENIMWSSDYPHPVSSWPKSRAMVQEM
                     FGDADPADRELVVCGNAARVWNL"
BASE COUNT          392 a          917 c          880 g          424 t
ORIGIN      
        1 ggaaccggcg aggacgcggc ggaggcgcgc gccgcgttcc tcgagcggcg gccgccccaa
       61 gtccggggga gctgatccgc ggcacctgac gcgacccgta ggtcgcgcta cggccgcgcg
      121 gcgagcaccg agtcgtcgaa cccgaacaac gtccgtgcgt tgccgctcgt gatcgcgatc
      181 gcgtcgcgct cgtcgacacc gacgaagaga tcgtcgagca ccttgttcga gtgcgggtac
      241 gtgctctccg ggtgcgggaa gtcgttgccc cagagcagcc catcggtacc ggtgagcggg
      301 atgttgtgca cggcgacggg gtcggtctgg aacgtcgcgt ggatctgccg ccggatgtag
      361 tggctgggca gctcgtcgag cttcggcttc gaccagctct cgtgcgcttg gtagtactcg
      421 tcgagcgtgc tcatcgtcca cgccagccag cccgcgttca cctccacgag cacgacatgc
      481 aacgcggggt gccgctcgag cacaccactg cagctgagca gtgcggtcgc gcgcgcggcc
      541 atcgactgtg tggtgagcag gttcgcggtt gcgctccccc acccgcgata gaagatcatg
      601 tcgtggccgg tgccctggtg catgaccacc ggtttcccga tctcggcgag cgcggcccac
      661 agcggctccc attgcgggag gttccactcc ggcgtgccga cgagcgggag cagtagccca
      721 ccgatcgacg ggtcgtcgcc gacgcgcgcg atctcggcca acgcgagctc gagcgtccag
      781 gtgggcagca tcgcggcgag cttgatgcgc gactcgccgc cgagctgctc gcggatccag
      841 tcgttgtaga tccggcatcc ggcgtcgccc accgcaggat cggcgacgtt ccacatgtac
      901 agcccgatgg tcgggtagat cacttcggcg ttcacgccgt cgcgccgcat catctcgaat
      961 cgcacgcgcg gtgacgcggc ggcggcgcgg tcggcttcga gcgcgcgagc ctcggtgacg
     1021 gacgcgtcgt cccacccgac cgcgtcaccg ttcacgacga gggtccagcc gccttcttcg
     1081 ttcccctgga tgcgccgcgg cgcgccctcg cgcagcgact ccgggagccg ctcgtaccag
     1141 aggtcgtgcg gctcgtcgac gtgagcgtcg gccgagatct tgggcaggtc ggcggcgagc
     1201 atcgcccgcg aggttaggcc cacatcccgc ttcttgcgcc gcgtgcggcc cccatgcgta
     1261 cgcacgcgac gcaagaacgg ttgacaggct cgggcggcgc cgtacatagt gatgactatg
     1321 tcgacgacta cgtcctctga acgtctcatc tcggccgacg accacgtgga cgtgagccac
     1381 gacgcggtca agacccacct ggcctcgaag taccacggcg cgtacgacgc cgggctcatg
     1441 gccttcggag cctcgatggg cagcacgatg tcggtcgagg cgaacagccg gtggcgcgac
     1501 cagcagaacc tcggggagcc cgagggcccc atcggcatgg gcgtgaaccg ccggcaccct
     1561 gcgagcggtc gcgccgggca ctcggacgcg aacgcgcgcc tcgcggacat ggatgtcgac
     1621 ggcgtcgagg cctcggtgag ctactgcgag gtgtcggcgt tccggtacct gtacctcatc
     1681 gaggacgggt ggcgggagtc gacgcgcgcg ttcaacctcg cgctcgccga cttcgcgtcc
     1741 gcggacccga agcgactcat cgtcaactac cagatcccga tccacgacat cgacgccgcg
     1801 gtcgacgaag tgcgctgggc ggcgtcggcc gggtgcaaat cgctgcagct ccccgtgtat
     1861 cccgccgagc tcgggctccc cgactactgg gacgtgcgct acgacccgct ctggaacgcg
     1921 atcacggacg ccgacctccc gatctgcctg cacatcggct tgaacaccgc gctcgacgac
     1981 ctcgcgcgcc gcgaccccac gccgcagaag ggcatcttcg tcccgttggt tgcgctgtcc
     2041 gccgccgagc cgctcggcat gtggatcatg ggcggcgtct tcgagcgaca cccgttgctc
     2101 aaggtggtgt tcgtcgagcc gggcctcgga tgggtcgcgt ggtggctcta catcgtcgac
     2161 gacctcgcgg ctcgccaggg ctacgacttc cctgcgatca cttacgcgcc gagccactac
     2221 ttccaccgca acgtgttcct cacattcatc gacgaaccgc acgcgatccg gatggccaac
     2281 gagacgctcg gcatcgaaaa catcatgtgg tcgtcggact atccgcatcc ggtgtcgtcg
     2341 tggccgaagt cgcgcgcgat ggtgcaggag atgttcggcg acgccgaccc cgccgatcgc
     2401 gagctcgtgg tctgcggcaa cgcggcgcgc gtctggaacc tctagctcga catgtccgct
     2461 cttggcgcct tcggcgccgg gccgctcagg ccacggctcg caacgaagcg acctctctgc
     2521 cgcatggctc gcctctgatg ccgggcgtct acgacggctt gaccgtcctc gacctctcgt
     2581 gggggacggc gggcccgatg acgaccatgt tcc
//
LOCUS       DASUGA010000139         2143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1296952, whole genome shotgun sequence.
ACCESSION   DASUGA010000139 DASUGA010000000
VERSION     DASUGA010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296952"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="VFX21_02700"
     CDS             complement(<1..1070)
                     /locus_tag="VFX21_02700"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5094890.1"
                     /translation="MTALAQAEDGLTVALRLVDDVRECGVVVTPAQSSLFVQALAAAP
                     DASLRHIARAALARTPDDLAIVDDVLGRVRHLRDRSPEIRRVAETFADGGDAHASDRR
                     RTSGRHMASTTEVLRTRDVAACRDDERAQIERMLAGMPLMGDRYRSRRLARSHGRGRL
                     DVRATVRHAARHDGEVVHVVRRGRAERTRRVVLLIDVSGSMERYARAMLLLAYVGAIG
                     AGNVEVFALGTRLTRVTQALSSRDPDVALRAASIAIEDWGGGTRLGETMRSLVDDPGC
                     RGLLRGAAAVIVSDGLDRGDPEQLRVHMARLSRLAHRVIWVNPLRASAGYEPLAAGMR
                     AALPHVDHFVDGHSVTAFDELVR"
     gene            complement(1067..1966)
                     /locus_tag="VFX21_02705"
     CDS             complement(1067..1966)
                     /locus_tag="VFX21_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005462990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5094891.1"
                     /translation="MQLPTADTVDDVVRCLADHEYLADEGLATAVLLAARLERPLFVE
                     GEPGTGKSELARVLSRWTGGELIRLQCHEGIDVAQAAYEWDYTRQLLHVRVGEAAGFG
                     AEQIEDSLFDERFLVRRPLLRAIEPREGPVPVLLIDEIDRADHEFDAFLLELLGDWAV
                     TIPELGTVRAARPPIVIITSNRTRDVHDALKRRCLYHWIEHPDREREAAIIRLRVPEA
                     TDAVVQAVADATERLRGLGLFKPPGVAEAIDWAAALAVLGRGVDTPAQMASGLRATLG
                     AAVKYHEDVQTVRDHLDEVVGQS"
BASE COUNT          343 a          766 c          725 g          309 t
ORIGIN      
        1 cgcaccaact cgtcgaaggc tgtcacggaa tggccgtcga cgaagtgatc gacatgggga
       61 agcgcggcgc gcatccccgc ggccagcggt tcgtatccag ccgaagcacg aagcgggttg
      121 acccagatga cgcgatgcgc aaggcgggac aggcgcgcca tatgcactcg cagctgctct
      181 ggatccccac ggtcgagtcc gtccgacacg atgaccgcgg cggcgccgcg cagcagaccc
      241 cggcatccag ggtcgtctac gagcgatcgc atcgtctcac ccaagcgcgt gccgccaccc
      301 cagtcctcga tcgcgatgct ggccgcgcgc agcgcgacgt ccggatcgcg ggacgacagg
      361 gcctgcgtca cgcgcgtgag gcgagtgccg agggcgaaca cctccacgtt gcctgcaccg
      421 atggcgccca cgtaggcgag gagcagcatc gcccgcgcat atcgctccat cgagccgctc
      481 acatcgatga gaagcacgac gcggcgagtg cgttcggcac ggccccgccg cacgacgtgc
      541 accacctcgc catcgtggcg cgccgcgtgt cgcaccgtcg cccgcacgtc gaggcgacca
      601 cgtccgtgcg agcgcgcgag acgtcgggat cggtagcgat cgcccatcag cggcatgccc
      661 gccaacatgc gctcgatctg cgcccgttcg tcgtcgcggc acgcggccac gtcacgtgtg
      721 cgcaggacct cggtggtgct ggccatgtga cgaccgctcg tgcgccgccg atccgacgcg
      781 tgcgcgtcgc cgccgtcggc gaacgtctcc gccacgcggc ggatctcggg cgaccggtca
      841 cggaggtgtc gaacgcggcc gagcacgtcg tccacgatgg cgaggtcgtc gggtgtccgc
      901 gccaacgcgg cacgagcaat gtgccgcagg ctggcatcgg gtgcggccgc gagggcctgt
      961 acgaacaacg acgactgcgc gggcgtcacg acgactccac actcgcggac atcgtcgacg
     1021 aggcggagcg cgaccgtgag gccgtcctcg gcctgtgcga gtgccgtcat gactgcccga
     1081 ccacctcgtc cagatggtct cggacggtct gcacatcctc gtggtacttc accgctgcgc
     1141 cgagcgtggc tcgcaggcct gaggccattt gcgcgggcgt gtcgacgccc ctgcccagca
     1201 ccgccagcgc cgcggcccag tcgatggcct ccgcgacacc gggcggcttg aacaacccga
     1261 gaccgcggag ccgctcggtc gcgtcggcga cggcctgcac caccgcgtcg gttgcttcgg
     1321 gcacccggag ccgaatgatg gcggcctctc gctcacgatc aggatgctcg atccagtggt
     1381 acaggcagcg acgcttgagc gcgtcgtgga catcacgcgt gcggttcgag gtgatgatca
     1441 cgatcggcgg tcgcgcggcg cgaaccgttc cgagctccgg gatggtgacc gcccaatcgc
     1501 ccaagagctc gagcaagaac gcatcgaact cgtggtcggc gcgatcgatc tcgtcgatca
     1561 ggagcaccgg gacgggtccc tcgcgcggct cgatggcgcg caagagcggt cgccgtacga
     1621 ggaaccgctc gtcgaacaga ctgtcctcga tctgttcggc cccgaatcct gcggcctcgc
     1681 caacccgcac gtgcagcagc tgacgggtgt agtcccactc gtaggcggct tgcgcgacgt
     1741 cgatgccttc gtggcactgc agtcgaatga gctcgccgcc ggtccaccga gacagcaccc
     1801 tcgccagctc gctctttcct gttcccggct cgccttcgac gaacaacgga cgctcgagcc
     1861 gcgccgcgag cagcaccgcg gtcgcgagcc cctcgtcggc gaggtattcg tggtcggcca
     1921 agcaccgaac gacgtcgtcg acggtgtcgg ccgtgggcaa ctgcacgggc aaagggtacc
     1981 gaggggcgcg gcgaccctgc ggtgatgatc tgactcagtc ggtcagccag atcagcgctt
     2041 ccgcgccgtt gtcgccggcg gtgatgcgcc gcgagcccgc gccggtgagg cgagccgaat
     2101 caccttcatc gagctcgatg tccggttcga gcaccaccga ccc
//
LOCUS       DASUGA010000140         3465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_472437, whole genome shotgun sequence.
ACCESSION   DASUGA010000140 DASUGA010000000
VERSION     DASUGA010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472437"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1546)
                     /locus_tag="VFX21_02710"
     CDS             complement(<1..1546)
                     /locus_tag="VFX21_02710"
                     /inference="COORDINATES: protein motif:HMM:NF016732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEX5094892.1"
                     /translation="MDLAKLLPPVPGRAVVPHRNGNGIKSTTGVSFVGAPDWRRVIHL
                     DGGSHPDAKRLTRAIALATSCYAYSAMTYRAQKIAEPPLMVVRDTDDGDAVFVEHELN
                     ELLDEPSLDYDMGVLLQLTEYYMLIGGQALWVKNDDLAGRMARLTPYCDTEFDSRGDE
                     QRIYAKYEVSTKRGRVTYDPEDVVHFRDPNPSSWRKGLAKLDVALALLDLGHQVERTV
                     RNFMMKAMFPGGIISPNADWEPEDDEWDQYVEAINAWHGGGADNSGEPLVLLGGTTFS
                     RAAVPLKELLPGEILDRIEATVGSVMGPPPVVLGWKVGLENSPWSNMKEARQSTYEET
                     LIPRMHFYEKALTKSMLTPEERKSGLRIAYDLSGVIALREDDERRAKVAASMRNEWTL
                     DERRVYTGKEPIGGEEGAQIGSAAPPVVELSLPAQARWVRDVVPFDQQTEVASRLAKL
                     GMKSVTSGPYRYMGTNGRFIRRQPRRGNVYRSSKGLVEWVMFDVGTKANERTWAVQVA
                     KWLKQAE"
     gene            complement(1676..3109)
                     /locus_tag="VFX21_02715"
     CDS             complement(1676..3109)
                     /locus_tag="VFX21_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEX5094893.1"
                     /translation="MKRRPEDILDKLLAGVDLGDTTATAERDMVETVLRRFHPDLERA
                     DAEREMERRARQAEGHGVREERALVPGVDLPQYAERLDEKWRYKVLYGGRGGAKSWTV
                     AAKLVEEGARTPIRVLCTREVQATIKDSVHKLLKDTIGRLGYLRYRITDNSIKHPIGT
                     EFIFKGLHDPDGLKSAEGVDRVWIEEAQRVTAHSWSVLPPTIRKPGSQIYVVFNPDLE
                     SDPTYQRFVVNPREDALLLKVGWEDNPWFAETELPAEKDHDYRVDPDLAAHIWEGEVR
                     KGHDAQILKNKYVSYAFEPQADWDGPYQGLDFGFAVDPTVSTRCWMHGRRLYVEYEAY
                     RIGLELDETAEFLCAQTADFEKYVTRADSAEAQAISYLKRKGLPKIDPVDKWSGSVED
                     GIRWFRQLESIVVHPRCAHTLRECRLYSYKTDRRTGDVLPEIVDAHNHCIDSIRYGLQ
                     PVIKYTKKKVRAWTPGIAGRAPLQRVR"
     gene            complement(3132..>3465)
                     /locus_tag="VFX21_02720"
     CDS             complement(3132..>3465)
                     /locus_tag="VFX21_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094894.1"
                     /translation="LLDHLRSLQTMIQDGAHADETWDAILVVFDRIKSGAEVHRRMLI
                     DAGEMVERSRVLMLIDRLSTDMIAVINEMEVADEQKREARIRVSNLVGTYLNRASLPP
                     SRNAPTPD"
BASE COUNT          536 a         1168 c         1094 g          667 t
ORIGIN      
        1 cctccgcctg cttcagccac ttggcgacct gcaccgccca cgttcgctcg ttggctttgg
       61 tgccgacgtc gaacatgacc cactcgacca accccttgct gctgcggtac acgttgccgc
      121 gacgcggctg ccgccggatg aagcggccgt tcgtgcccat gtaccgatac gggcccgacg
      181 tcactgactt catcccgagc ttcgccagcc gcgacgccac ctccgtctgt tggtcgaatg
      241 gcacgacgtc acgcacccag cgcgcttgcg ccggcagaga cagttccacg acgggcggcg
      301 cggcggaacc aatctgcgcg ccttcctcgc cgccgatcgg ttccttgccg gtgtatacac
      361 ggcgctcgtc caacgtccac tcgttgcgca tggacgctgc gaccttagcg cggcgctcgt
      421 cgtcttcgcg cagcgcgatg acacccgaca ggtcgtacgc gatgcgcagc cccgatttgc
      481 gctcctcggg ggtgagcatc gacttagtca gcgccttttc gtagaaatgc atacgaggga
      541 tgagcgtttc ctcgtacgta ctttggcgcg cctctttcat gttggaccac gggctgttct
      601 ccaacccgac cttccagccc agcaccacgg gcggcggccc catgaccgag cccacggttg
      661 cttcgatgcg gtcgagaatt tcgccgggca acagttcctt caacggaacc gctgcgcgcg
      721 agaacgtcgt gccacccagc aacaccagcg gttcgcccga gttgtccgcg ccgccgccgt
      781 gccacgcgtt gatcgcctcg acgtactggt cccactcgtc gtcctcgggt tcccaatcgg
      841 cgttaggtga gatgatgccg ccggggaaca tcgccttcat catgaagttg cggacggtgc
      901 gttcgacttg gtggccgagg tcgagcagcg cgagcgcaac gtcgagcttg gcgagcccct
      961 tacgccacga cgacgggttc gggtcgcgga agtgcacgac gtcttcgggg tcgtatgtca
     1021 cccgaccgcg cttggtcgac acttcgtact tggcgtagat gcgctgctcg tcgccgcgcg
     1081 agtcgaactc ggtgtcgcag tacggcgtca accgcgccat gcgccccgcg aggtcgtcgt
     1141 tcttgaccca caaggcttgc ccaccgatga gcatgtagta ctcggtgagt tggagcagca
     1201 cgcccatgtc gtagtcgagc gacggctcgt ccagcagttc gttcaactcg tgctcgacga
     1261 acacggcgtc gccgtcgtcc gtgtcgcgca cgaccatgag cgggggttcc gcgatcttct
     1321 gcgcgcggta cgtcatcgcg gaatacgcgt agcacgaggt cgcgagcgcg atcgcgcggg
     1381 taagtcgctt ggcgtcgggg tgggacccgc cgtcgaggtg gatgacgcgc cgccagtcag
     1441 gcgcgccgac gaagctcacc ccggtcgttg acttgatccc gttgccgttc cggtgtggta
     1501 cgacggctcg tccggggacg ggtggcaaga gctttgccag gtccacgcgg accaccttat
     1561 cgctcatcag gggacgcagc ttttggcagg gggcaacgac gacgtgcacg cccggccgag
     1621 cgtacgcgtc ggacgacacc ttacactggg cggccccggc ctgtaaaccc ccacgtcacc
     1681 ggacgcgttg gagcggggcg cggccggcga tgccgggcgt ccacgcgcgg accttctttt
     1741 tcgtgtactt gatgacgggt tgcaggccgt atctgatcga gtcgatgcag tggttgtgcg
     1801 cgtcgacaat ttccggcaac acgtcgcccg tgcgccggtc ggttttgtag gagtacagcc
     1861 ggcactcgcg caacgtgtgt gcgcaacgcg ggtgcacgac gatggactcc aactggcgga
     1921 accagcggat gccgtcttcg acggagcccg accacttgtc gaccgggtcg atcttcggca
     1981 ggcccttccg cttcaggtaa ctgatcgcct gcgcctcggc gctgtcggcg cgcgtcacgt
     2041 acttctcgaa gtctgccgtt tgcgcgcaca ggaactcggc cgtttcgtca agctccaacc
     2101 cgatgcgata cgcctcgtac tcgacgtaca gccggcggcc gtgcatccag caccgcgtcg
     2161 acaccgtcgg gtcgaccgcg aacccgaagt cgagcccttg gtacgggccg tcccaatccg
     2221 cctgtggctc gaacgcatac gacacgtact tgttcttgag gatttgcgcg tcgtgtccct
     2281 tgcgcacctc gccttcccag atgtgcgcag cgaggtcggg gtccacgcgg tagtcgtgat
     2341 ctttctcggc gggcaactcc gtctcggcga accacgggtt gtcttcccag ccgaccttca
     2401 acaacaacgc gtcttcacgc gggttcacga cgaaccgctg gtaggtgggg tcggactcca
     2461 ggtcggggtt gaacacgacg taaatctgcg acccgggctt acgaatcgtg ggcggcaaca
     2521 ccgaccacga gtgcgccgtg acgcgttgcg cttcttcgat ccacacgcgg tcgacgccct
     2581 cggctgactt caacccgtcg gggtcgtgca accctttgaa gatgaactcg gtgccgatcg
     2641 ggtgcttgat cgagttgtcg gtgatgcggt accgcaggta acccagccgg ccgatcgtgt
     2701 ccttgagcag cttgtggacc gagtctttga tcgtcgcctg cacctcgcgt gtgcacagca
     2761 cgcggatcgg cgtgcgcgcc ccttcctcga cgagcttggc cgcgacggtc cacgacttcg
     2821 cgccaccgcg cccgccgtac agcaccttgt accgccactt ctcgtcgagc cgttcggcgt
     2881 attgcggcag gtcgacgccc ggcaccaacg cgcgctcctc gcgcacgccg tgtccctcgg
     2941 cttgccgtgc gcggcgctcc atctcgcgct cggcgtcggc gcgttcaagg tcggggtgga
     3001 accgacgcaa cactgtctcg accatgtcgc gctcggccgt cgcggtcgtg tcacccaggt
     3061 cgacgccggc caacagtttg tcgaggatgt cctcgggccg acgcttcaac ccatcgagca
     3121 gcgcgcgtcg gtcagtccgg cgtcggggca tttcgtgaag gcggcagtga ggcgcggttc
     3181 aggtacgtgc cgacgaggtt cgacacgcgg atgcgggctt cgcgtttctg ttcgtcggcc
     3241 acctccatct cgttgatgac ggcaatcatg tcggtcgaca gccggtcgat caacataagc
     3301 acgcgcgacc gttccaccat ctcgcccgcg tcgatcaaca tgcggcggtg gacttcggct
     3361 cccgacttga tgcggtcgaa cacgacaaga atggcgtccc acgtttcgtc cgcgtgcgcg
     3421 ccgtcttgga tcatggtctg caacgaacgc aggtggtcga gcagt
//
LOCUS       DASUGA010000141         3652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1019268, whole genome shotgun sequence.
ACCESSION   DASUGA010000141 DASUGA010000000
VERSION     DASUGA010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019268"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="VFX21_02725"
     CDS             complement(<1..1322)
                     /locus_tag="VFX21_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094895.1"
                     /translation="MGTSTGTGTLGGPTFGGPTGGFTVGSGGSGAIDNMGLGGAGAGG
                     DNSCAGEEHEAELIPLDVYVMLDRSLSMEASTASGVTKWEAITSALKAFVADPESDGI
                     GVGIQYFPTNLPCTSNDECDEGLCYTKACRISRSMSGNLRGLIPCLGDDDCPESGDSC
                     VDLGGCGDESCVSLGDSCDNDIECTAVESGVCASQSVCTLSDYTTPEVPIGILPDSSQ
                     ALLDSLDAYSPAPNPFGLTPTGPALEGAIAYARSWAEEHPERKAIVVLATDGAPTGGC
                     SPSGRAEIAALASVGARATVPVQTYTVGVFAPEDPENPSEDSIEGPRTIRAIAEAGAG
                     QAFVISDQDDVAAQFVAALNSVRSHGLACEFQVPPSERGKPLDLGKVNVKFTPKAGED
                     AETIGYVEEASECGDEGGWYYYSDPSSNQPLSIRTCDATCTRLTSSLNG"
     gene            complement(1499..>3652)
                     /locus_tag="VFX21_02730"
     CDS             complement(1499..>3652)
                     /locus_tag="VFX21_02730"
                     /inference="COORDINATES: protein motif:HMM:NF036933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094896.1"
                     /translation="ENSDTAADACRENCVLPSCGDAVVDDAEGCDDGSDNSDFRADAC
                     RSDCTAAHCGDGLIDSTEDCDSDKFGRATCAREGFSSGVIACTETCTFDTSGCVTCGD
                     GVADGESESDPGYEECDTEDFRGKDCSDFGFDGGRLVCSECQIDSASCGAEPAGCGND
                     LTETGEVCDGTDLGDQDCSDFGFPGGYLACKSDCSDFDPSHCNSCGNGTIEGGEVCDD
                     GNAEDDFTCSADCRSSCGLGFGECDDDTSTYCGYDRRGKAAILTEYCDPAQGLSCTDG
                     RCRGACAVSALGSSYFGCDYYPTITNNALLVETSGDFAVTVANAGSATANVTVTMGEA
                     TIATATAAPGDVAVIVLPWTELRTASTILAVDGAYRLRSDQPVTVYQYNPLNYEKAGA
                     FTYTNDASLLLPTNTWTGNYVVVARNSTSSPGLYSVVAKEDDTEVTLIPSATATMVAT
                     IEGIAADGSGTVTLNAGDVLQVLAPVTGYTPDNDVTGTRIQATKPVQVFGGEECANIP
                     SGVSACDHLEEAMLPIETLGSSYFVTPPLVDAITPKARMVRVVAVEPDTTISYDPPQD
                     GAPTTLVNAGDYFEIDQTLASFEIKADRRIMVAEYMLGQGAGGGTGDPAMVLGVPVEQ
                     YRTNYLFHAPTNYEANYLNVTAPTGATVNLDGAALPAGTAIGASGFSAIQVPLDNSGN
                     GNHVVTATARVGISVYGYGQYTSYWYPGGLDLTQF"
BASE COUNT          581 a         1160 c         1224 g          687 t
ORIGIN      
        1 ccgttcagag agctcgtcag ccgcgtgcac gtggcgtcgc aggtgcgaat gctgaggggt
       61 tggttgctcg agggatccga gtagtagtac cagccgccct cgtcaccgca ctctgacgct
      121 tcctcgacgt acccgatcgt ctctgcgtct tcgccggctt tcggagtgaa cttgacgttg
      181 accttaccga ggtcgagcgg cttgccgcgt tccgacgggg ggacctggaa ttcacacgcc
      241 agcccgtggc tgcgtacgga gttcaacgcc gccacgaact gcgctgcgac gtcgtcctgg
      301 tcgctgatga cgaaggcttg cccggcgccg gcctcggcga tcgcgcggat ggtcctgggt
      361 ccttcgatgc tgtcctccga tgggttctcg gggtcttcgg gggcgaacac gccgaccgtg
      421 taggtctgca cgggaaccgt ggcgcgtgcg ccgaccgagg cgagtgccgc gatttcggcg
      481 cggccggatg ggctgcagcc gccggtcggt gcgccatcgg tagcgagcac gacgatggct
      541 ttgcgttccg gatgctcttc agcccacgag cgagcgtaag cgatggcgcc ttccaaggca
      601 ggcccggtgg gcgtcagccc gaacgggttc ggtgctggac tgtacgcatc gagcgagtcg
      661 agcagcgcct gagacgaatc cggcaagatg ccgatcggca cctcgggagt cgtgtagtcc
      721 gagagcgtgc acacgctctg cgacgcacac acgccgcttt cgactgcggt gcactcgatg
      781 tcgttgtcgc agctgtcgcc gagcgacaca cacgattcgt cgccgcagcc gccgagatcg
      841 acgcaactgt cgccgctctc ggggcagtcg tcgtcaccga ggcatgggat caggcctctt
      901 agattcccgc tcatgctgcg actgatgcgg cacgccttcg tgtagcacag gccctcgtcg
      961 cattcgtcgt tgctggtgca cggcaggttc gtcgggaaat actgaatgcc gacgccgatg
     1021 ccatccgact ccgggtccgc cacgaaggcc ttgagggccg aggtgatcgc ttcccacttc
     1081 gtgacaccgc ttgctgtcga cgcctccatg gacagcgagc gatcgagcat gacgtacacg
     1141 tcgagcggga tgagctcggc ctcgtgctcc tcgccggcgc agctattgtc gcccccggcc
     1201 ccggcaccgc cgagccccat gttgtcgatg gcgcccgaac cgccagagcc cacggtgaaa
     1261 ccgccggtgg ggccgccgaa tgtcggaccg cccagcgtgc ctgtgccggt cgaggtgccc
     1321 acgccccccg tactcgtcac gtcaaagatg gtgtcggact cgctagggtt gtcctccttt
     1381 ttgcatgcaa acgggatgac aacgagcgcc gtcacgactc cgaataggcg ccgcgctcgc
     1441 gggtgacggg ttctggtggc ttgagtagtg attggatagg acatggttaa acctgcgttc
     1501 aaaactgggt aaggtcgaga ccgccggggt accagtagct ggtgtactgc ccgtagccgt
     1561 agaccgaaat gccgacgcga gccgtcgcgg tgacgacgtg gttgccgttg ccggagttgt
     1621 cgagcggcac ttgaatcgca ctgaaccccg atgccccaat ggcagtgccg gcgggtagtg
     1681 cagcgccgtc caggttgacc gtggcaccgg tcggcgcagt gacgttcagg tagttggcct
     1741 cgtagttggt aggcgcgtgg aacaggtagt tggttcggta ctgttcgacg ggcacgccga
     1801 ggaccatggc cgggtcgccc gtgcccccac cggcaccctg gcccagcatg tattcggcga
     1861 ccatgatgcg acgatcggct ttgatctcga acgaagccag cgtctggtcg atctcgaagt
     1921 agtcaccggc gtttacgagc gtggtgggcg cgccgtcttg cggcgggtcg tagctgatgg
     1981 tcgtgtcggg ttcgacggcg acgactcgca ccatgcgtgc cttgggggtg atcgcatcga
     2041 ccaaaggcgg tgtcacgaag tacgacgagc ccagcgtctc gatgggcaac atcgcttctt
     2101 cgaggtggtc gcacgccgat acgcccgatg gaatgttggc acattcttcg ccgccgaaga
     2161 cttggaccgg cttcgttgct tgaatgcgcg tcccggtgac gtcgttgtcc ggcgtgtaac
     2221 cggtcacggg cgccaggact tggagcacgt cccccgcgtt gagcgtcacg gtgcccgagc
     2281 cgtcggcggc aatgccttcg atggtggcca ccatggtggc ggtggccgag ggaatcagcg
     2341 tgacttcggt gtcgtcttcc ttcgccacta ccgagtagag ccccggagag ctggtcgagt
     2401 tgcgcgcgac gacgacgtaa ttgcccgtcc acgtgttggt cggcaagagc agcgaagcgt
     2461 cgttcgtgta ggtgaaagcg cctgcttttt cgtagttgag cgggttgtac tgatagacgg
     2521 ttaccggctg gtcgctgcgc aaccggtaag caccgtcgac agcgaggatg gtactggcgg
     2581 tgcgtagctc ggtccacggc agtacgatca cggcgacgtc gcccggggcg gccgtagcgg
     2641 tggcgatcgt tgcctctccc atggtgacgg tcacgttcgc agtcgccgag ccggcattgg
     2701 ccaccgtcac cgcgaagtcg cctgaggtct cgacgagcag cgcattgttg gtgatagtcg
     2761 ggtagtagtc gcagccgaag taactcgagc ccagggccga gacggcgcac gcgccccggc
     2821 agcggccgtc cgtgcaactc aagccctggg ctgggtcgca gtactcggtg aggatcgcag
     2881 ctttgcccct gcgatcgtag ccacaatagg tggacgtgtc gtcgtcgcac tcaccgaagc
     2941 cgaggccgca gctcgagcgg cagtcggccg agcacgtgaa atcgtcctcg gcgttgccgt
     3001 cgtcgcagac ctcgccgccc tcgatcgtgc cgttgccgca cgagttgcag tggctcgggt
     3061 cgaagtccga gcagtcgctc ttgcacgcga ggtagccacc cggaaagcca aagtcggagc
     3121 aatcctgatc acccagatca gtgccgtcgc agacttcgcc agtctcggtc aggtcgttgc
     3181 cgcatcccgc aggctctgcg ccgcagctcg cgctgtcgat ctggcactcc gagcacacga
     3241 gccggccgcc gtcgaacccg aaatcgctgc agtctttgcc gcggaaatct tcggtgtcgc
     3301 actcttcgta gccggggtcg ctttcgctct cgccgtcggc gacgccgtcg ccgcacgtaa
     3361 cgcagccaga ggtgtcgaag gtgcaggtct cggtgcacgc gatcaccccg gagctgaagc
     3421 cttcgcgcgc gcacgtcgcg cggccgaact tgtccgaatc gcagtcttcg gtactgtcga
     3481 tcaagccgtc gccgcagtgc gcggccgtgc aatcgctgcg gcaagcgtcg gcgcgaaagt
     3541 ccgaattgtc cgatccgtcg tcgcagccct cggcgtcgtc gacgaccgcg tcgccacagc
     3601 ttggcagcac acaattctct cggcacgcgt ccgccgccgt gtcggagttc tc
//
LOCUS       DASUGA010000142         3549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_480703, whole genome shotgun sequence.
ACCESSION   DASUGA010000142 DASUGA010000000
VERSION     DASUGA010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480703"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..385
                     /locus_tag="VFX21_02735"
     CDS             128..385
                     /locus_tag="VFX21_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094897.1"
                     /translation="MRHITPGRLSQIEGLLVGVRRCNGLTERKPGTFYKKSSAFLHFH
                     EHGDDEVYADCKLDGRTFERVRVTTKREQDALLRRIRKALA"
     gene            397..1008
                     /locus_tag="VFX21_02740"
     CDS             397..1008
                     /locus_tag="VFX21_02740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5094898.1"
                     /translation="MDQQLLEVARAAKGFMPDDEGLALYEAGRAAGARGPLLEIGTYC
                     GKSAVYLGAAAREAGTLLFTVDHHRGSEENQAGWEHHDTDVVDPGTGRMDTLPFFRRT
                     IATAGLEDVVVGVLGHSVPVARVWRTPLAFLFIDGGHAEDVAMADYEQWIPHVMAGGT
                     LAVHDVFERPEDGGQAPFHVWQRAVGDGFTPAGTTGSLRLLHR"
     gene            1078..1590
                     /locus_tag="VFX21_02745"
     CDS             1078..1590
                     /locus_tag="VFX21_02745"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5094899.1"
                     /translation="MAESDGGHALEAQFEELFRASYAGLVRSLTVAAGSQEVAADCVQ
                     EAFCRAYVRWRRVGRYDEPVAWVRRVAVNLLIDHSRRMQRHDRALARMPVDVEPPPSD
                     PLEVDLEGALDSLTPQQRVAAALFYVEDLPVTEVAAAMKLSEGAVKYHLHQARATLRD
                     TLDAHREDIS"
     gene            1587..2300
                     /locus_tag="VFX21_02750"
     CDS             1587..2300
                     /locus_tag="VFX21_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094900.1"
                     /translation="MSPNEFDDGELRGGLQDLAPDVDVRSALDALRPRARRARTRHRA
                     RLAAGVTAGILLVGGGAVALTNTSNDKVSITPATSPKIADDTSTTTVPPPTTPTTANP
                     DTTSPTAAPTDATTPDSSPPGAPSDSRQLPTQSTAPTSPTTDHGRGNDTAPDTEVRTY
                     SSAGGSIDVHVAGGVLMLDDVRPAGGYDEAADKRVERDDRIRVEFERKDGNHQGQPPR
                     VEVRIINGELVQTPVDPSS"
     gene            2335..2904
                     /locus_tag="VFX21_02755"
     CDS             2335..2904
                     /locus_tag="VFX21_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094901.1"
                     /translation="MIRVGMTKRLVAIGVATVALGGTAYAAATVLPADDKGVEPRSEA
                     ADDSSTSTSVDDSSTTSTVGATSTTIDETTTSSVDTTPSSVDDTTPDSADDTTPNSID
                     DDACHHGARVSEIARNTPPGPEHGKIVSDAAHQKCDDEGDVEDGEHRNRHSGDDDEQV
                     DDNDNNGHRNGGAEHDAEDNSGHGRGGDD"
     gene            complement(2905..>3549)
                     /locus_tag="VFX21_02760"
     CDS             complement(2905..>3549)
                     /locus_tag="VFX21_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094902.1"
                     /translation="KGALLTGSSSIYSSVRCAIAAAERLGQERPDWELSLGSLAIAIA
                     HRPERFLEKERWAMDWYYPILGGVLRGHAAEARIASRWDTFVVPGRGVRCVSDQPWVT
                     AAETCELVMALDAIGLDERAHALFRDVQFLRHDDGAYWTGMNFDGDRFDESGEYYTAE
                     HPTWNSAAVVLAANALGGNGPTSGLFRGVGLPAGLTADELIDAGAEIEAARRAG"
BASE COUNT          626 a         1231 c         1214 g          478 t
ORIGIN      
        1 gacgcgaacc tgatgccggc gttgctcgac gcggtgcggg cctacgcgac gctcggcgag
       61 atcgtcgacg cgctcgccga cgtcttcggc cgttgggtcg agacgccgcg tatctaggca
      121 tcgcacggtg cggcacatca cgcccgggcg gctgtcgcaa atcgagggcc tactcgttgg
      181 tgtgcgccgg tgcaacgggc tgaccgagcg caagcccggc acgttctaca agaagtcgag
      241 cgcgttcttg cacttccacg agcacggcga cgacgaggtg tacgccgact gcaagctcga
      301 cggccggacg ttcgagcggg tccgggtcac gaccaaacga gagcaggacg cgctcctgcg
      361 gaggatccgc aaagcgttgg cctagcgtca ccggcgatgg atcagcagct gctcgaggtc
      421 gcccgcgcgg ccaaggggtt catgcccgac gacgaagggc tcgcgctgta cgaagccggc
      481 cgcgccgcgg gcgcacgcgg gccgttgctc gagatcggca cgtactgcgg caagtccgcg
      541 gtctacctcg gcgccgccgc gcgcgaagcc ggcacgttgc tcttcaccgt cgatcatcac
      601 cgtggttcgg aggagaacca agcgggctgg gagcatcacg acaccgacgt cgtcgacccc
      661 ggcaccggcc gcatggacac cctgccgttc ttccggcgga cgatcgcgac ggccggtctc
      721 gaagacgtcg tggtgggcgt gctcggccac agcgtcccgg tcgcccgcgt ctggcggacg
      781 ccgctcgcgt tcttgttcat cgacggcggc cacgccgaag acgtcgcgat ggccgactac
      841 gagcagtgga tcccgcacgt catggccggc ggcaccctcg ccgtccacga cgtcttcgag
      901 cgccccgaag acggcggcca ggcgccgttc cacgtctggc agcgggccgt gggcgacggc
      961 ttcacgcccg ccggcacgac cggatcgctg cgccttctgc accgttgact ctccgcggtc
     1021 gattcctcgc cgctgagcca aaccggaagc gggtttggag cgtggtaatc aacggacatg
     1081 gcggaatccg acggcggcca cgccctcgaa gcgcaattcg aggagttgtt ccgcgcgtcg
     1141 tatgccggtt tggtccgcag tttgacggtt gccgcgggat cgcaggaggt ggcagcggac
     1201 tgtgtgcaag aggcgttctg tcgtgcgtac gtgcgctggc gccgggtggg tcgctacgac
     1261 gaacccgtgg catgggtgcg gcgggtcgca gtgaacctgc tcatcgatca ctcgcgccgg
     1321 atgcaacgcc acgaccgcgc gctcgcgcgc atgccggttg acgtcgagcc gccgccctcg
     1381 gatccactcg aggtcgatct cgagggcgcg ctcgattcct tgacgccgca gcagcgcgtc
     1441 gcggccgcgc tgttctacgt cgaagacctt cctgtgaccg aggtcgcggc ggccatgaag
     1501 ctcagcgaag gcgcggtcaa gtaccacctg caccaagcgc gcgcgacctt gcgcgacacc
     1561 ttggatgcac atcgcgagga catctcgtga gcccgaacga attcgacgac ggcgagctgc
     1621 gcggcggact gcaagacctc gcgcccgacg tcgacgtccg cagcgcgctc gacgcgcttc
     1681 ggccgcgtgc gcgccgtgcc cggacccgcc accgggcacg gctcgccgcg ggtgtcacgg
     1741 ccggcatcct gctggtgggc ggtggtgcgg tcgcactgac gaacacgagc aacgacaagg
     1801 tctcgatcac gcccgcgaca tcaccgaaga tcgccgacga cacatcgacg acgacggtgc
     1861 cgccgccgac gacgcccacg accgcgaacc cggacacgac gtcaccgacg gccgcgccga
     1921 ccgatgcgac gacacccgat tcgtcgccgc ccggagctcc cagcgattcg cggcagctgc
     1981 cgacgcagtc gaccgcaccg acgtcaccga cgaccgatca cgggcgcggg aacgacaccg
     2041 cgccggacac cgaagtgcgg acgtactcca gtgccggagg cagcatcgac gtgcacgttg
     2101 ccggtggggt gctcatgctc gacgacgtac ggccggcggg cggctacgac gaagccgcag
     2161 acaagcgagt cgagcgcgat gaccggattc gcgtcgagtt cgagcgaaaa gacggaaatc
     2221 accagggcca gccgcctcgc gtcgaagtgc gcatcataaa cggagaactc gtccaaaccc
     2281 cggtcgaccc gtcgtcgtga gacctcctga acgggaactc aggaggaaga gcggatgatc
     2341 cgtgtcggga tgacgaagcg actggtggcc attggcgtgg caaccgttgc gttgggcggc
     2401 actgcatacg cagccgccac agtgctaccc gcggacgaca agggcgtcga accgcgtagt
     2461 gaggccgccg acgacagctc gacgtcgacg agcgttgacg attcgtcgac gacttcgacg
     2521 gtgggggcga cgtcgaccac gatcgacgaa accacgactt ccagtgtcga caccacaccg
     2581 agcagcgtcg acgacaccac tccggacagc gccgatgaca cgacgccgaa ctcgatcgac
     2641 gacgacgcct gccaccacgg cgccagggtc tccgagatcg cgcgcaacac gcctcctgga
     2701 ccggagcacg gcaagatcgt ctccgacgcc gcgcatcaga agtgcgacga cgagggtgac
     2761 gtcgaggacg gcgagcaccg gaaccgccac tcgggcgacg acgacgagca ggtcgacgac
     2821 aacgacaaca acggtcaccg aaacggtggc gcggagcacg acgccgagga caacagcggc
     2881 cacggccgcg gcggcgacga ctaactagcc ggcgcggcgg gcggcttcga tctcggcgcc
     2941 cgcgtcgatg agctcgtcgg cggtgaggcc ggccgggagt ccgacaccgc ggaacaaccc
     3001 cgacgtcgga ccgttgccgc ccagtgcgtt ggccgcgagc acgaccgcgg cgctgttcca
     3061 cgtcgggtgc tcggccgtgt agtactcgcc gctctcgtcg aagcggtcac cgtcgaagtt
     3121 catgccggtc cagtacgcgc cgtcgtcgtg gcgcaagaac tgcacgtcac ggaacagcgc
     3181 gtgcgcgcgt tcgtcgaggc cgatcgcgtc gagcgccatc accagctcgc aggtctcggc
     3241 cgcggtgacc cacggctgat cggagacgca gcgcacgccg cgcccgggga cgacgaacgt
     3301 gtcccatctc gacgcgatac gcgcttcggc cgcgtggccg cgcagcacac cgccgagaat
     3361 cgggtagtac cagtccatcg cccagcgctc cttctccaag aagcgctcgg gccggtgggc
     3421 gatcgcgatc gcgagcgaac cgagggacag ctcccagtcg gggcgttcct ggccgaggcg
     3481 ttccgcggcg gcgatcgcgc aacgcaccga cgagtagatg ctcgacgaac cggtcaacaa
     3541 cgcgccctt
//
LOCUS       DASUGA010000143         3317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1756283, whole genome shotgun sequence.
ACCESSION   DASUGA010000143 DASUGA010000000
VERSION     DASUGA010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756283"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /gene="hisH"
                     /locus_tag="VFX21_02765"
     CDS             complement(<1..399)
                     /gene="hisH"
                     /locus_tag="VFX21_02765"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX5094903.1"
                     /translation="MERVAIVDYKLCNLDSVQRAFETLGAQAFITDDPADLARADRIV
                     LPGVGAFPDAMRNLRAAGLDEGLGEHVLEGGAPFLGVCLGMQLIAAIGHEVEDTKGLG
                     WIDARVSRLEPTEQDRRIPHVGWNEVRAVRE"
     gene            complement(491..1708)
                     /locus_tag="VFX21_02770"
     CDS             complement(491..1708)
                     /locus_tag="VFX21_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HEX5094904.1"
                     /translation="MVQSVEGATQELIRNRKAEKRPIVYCRRCLIPETKPDIFFDDEG
                     VCSACRHYEQRGDVDWAARRVELDEVLERYRSKDGTTYDCVIGVSGGKDSTTQVIRML
                     ELGMNPLCVTATTDELSGIGRRNIENLKGLGVDFVEYTANPVVRRRINKLALVQVGDI
                     SWPEHVTIFTLPVRIAVQMGIKLIVWGENPQNEYGGPAAAATGNTLTRRWLEEFGGLL
                     GLRVSDLVGRDGIETRHLIPYTYPSDEDLARVGVTGIFLGYYMPWDGLQNAIFAQSHG
                     LETWPQMIEGSLCNYENLDNHQTGIHDYFKFIKYGFGRATDIASMHVRRDRLRRADAL
                     TLVKQHDGKFPWTYLGKPIEDVLANIDMSFDEFIAVCDRFTNKRLFVTDRHGELVRGA
                     DQHLTKINDDNVS"
     gene            complement(1714..2859)
                     /locus_tag="VFX21_02775"
     CDS             complement(1714..2859)
                     /locus_tag="VFX21_02775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5094905.1"
                     /translation="MRVTCVVQARTGSTRLPGKVLQPVDDRPMLRFMLDRLATVHVDM
                     LVVATSTLARDDVVADIGRDAGYRVVRGSETDVLGRFVTALDAAPADHVIRLTADCPL
                     IDPKLVETVVARHFECDADYTSNVFPRTFPKGLDVEVVRASVLRTAGEEATDPAEREH
                     VTPFVYRRPERFRLANVRNDQWLGGERWTVDTAEDLEFVRALVARMDDSAFSWRDAYK
                     IVGAQQVVTRGEVLLVPAAVEHRTFVHACRSDADAVRRSRSGRAVSAEDHARWYETAL
                     ADPAVRFRVAILDDEPIGTVRVDIVGGVGEVGIALLPGRRGEGLAARLLEALLADCRA
                     DPQVASLVATIHPENQPSIRAFAGAGFVRGADRDGFRVLHRPVHQPI"
     gene            complement(2859..>3317)
                     /locus_tag="VFX21_02780"
     CDS             complement(2859..>3317)
                     /locus_tag="VFX21_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094906.1"
                     /translation="TGADLAGAIHTAEPDLQVRLVIGPWGSQSIPDGVVGVQAPSSLS
                     GEMAGAQIVVSAGGVALLEACRLARAVVAVTMAENQRTAVEYLHRVGAVLAVADDEVA
                     TAVSGLRRDAAARRALARAAAEAIDGQGAARAAAQLVEQAADVRNPVRTF"
BASE COUNT          502 a         1162 c         1126 g          527 t
ORIGIN      
        1 ctcgcgtacg gcacgcacct cgttccagcc gacgtgcggg atgcgacggt cctgctcggt
       61 cggctcgagc cgcgacacgc gcgcgtcgat ccaaccgagg cccttcgtgt cctcgacctc
      121 gtgaccgatg gcggcgatga gctgcatccc gaggcagaca ccgaggaacg gcgcgccgcc
      181 ttcgagcacg tgctcgccga ggccctcgtc gaggccggcg gcgcgcaagt tccgcatcgc
      241 gtcggggaat gcaccgacgc ccggcaacac gatgcgatcc gcgcgcgcca agtcggcagg
      301 gtcgtcggtg atgaatgcct gcgcacccaa ggtctcgaac gcgcgctgca ccgagtcgag
      361 gttgcagagc ttgtagtcga cgatggcgac gcgttccatc acgtacttcc cgctcagagg
      421 gccgctcggg ccccggctcg ctggttcgcg cgactgcgtc tcgtcatggt cccgctcgcc
      481 tcagccgccc tcacgagacg ttgtcgtcgt tgatcttcgt caagtgctgg tcggcgccgc
      541 gcacgagctc gccgtgccgg tcggtcacga acaaccgctt gttcgtgaac cggtcgcaga
      601 ccgcgatgaa ctcgtcgaac gacatgtcga tgttcgccag cacgtcctcg atcggcttgc
      661 cgaggtacgt ccacgggaac ttgccgtcgt gctgcttgac caaggtcagc gcgtcggcac
      721 ggcgcaagcg gtcgcgccga acgtgcatcg acgcgatgtc ggtggcccgc ccgaacccgt
      781 atttgatgaa cttgaagtag tcgtggatgc cggtctggtg gttgtcgagg ttctcgtagt
      841 tgcacagcga gccctcgatc atctgcggcc atgtctccag gccgtgagac tgggcgaaga
      901 tcgcgttctg caggccgtcc cacggcatgt agtagccgag gaagatgccg gtcacgccga
      961 ctcgcgcgag gtcttcgtcg ctcggatagg tgtacgggat caggtggcgg gtctcgatgc
     1021 cgtctcggcc gacgaggtca ctgacccgca atccgaggag gccgccgaac tcctcgagcc
     1081 agcggcgcgt caacgtgttg ccggttgccg cggcggccgg gccgccgtac tcgttctgcg
     1141 ggttctcgcc ccagacgatc agcttgatgc ccatctgcac cgcgatgcgc acgggcagcg
     1201 tgaagatcgt cacgtgttcc ggccacgaga tgtcgccgac ctgcacgagc gcgagcttgt
     1261 tgatgcgccg gcggaccacg ggattggcgg tgtactcgac gaagtcgacg ccgagccctt
     1321 tcaggttctc gatgttgcgc cggccgatgc cggagagctc gtccgtcgtc gcggtcacgc
     1381 acaacgggtt catccccagt tcgagcatgc ggatgacttg ggtcgtcgag tccttgccgc
     1441 cgctgacacc gatcacgcag tcgtacgtcg tgccgtcctt cgagcggtag cgctcgagca
     1501 cttcgtcgag ctcgacgcgg cgcgcggccc aatcgacgtc accgcgttgc tcgtagtggc
     1561 ggcacgcgct gcagacgcct tcatcgtcga agaagatgtc gggcttcgtt tcggggatga
     1621 ggcagcgccg gcagtagacg atcggccgct tctcggcctt ccggttccgg atgagctcct
     1681 gcgtcgcgcc ttccacggac tggaccatga cgtctatatc ggctggtgca cgggacggtg
     1741 cagcacccgg aacccgtcgc ggtcagcgcc gcgtacgaac cccgcgccgg cgaaggcccg
     1801 gatcgacggc tggttctcgg gatggatcgt cgcgaccagg gaggcgactt ggggatcggc
     1861 ccggcagtcg gcgagcagcg cctcgagcag ccgagcggca aggccctcgc cccggcggcc
     1921 gggcagcagt gcgatgccca cctcgccgac accgcccacg atgtcgacgc gcacggtgcc
     1981 gatcggttcg tcgtcgagga tcgcgacccg gaaccgcacc gcagggtctg cgagcgcggt
     2041 ctcgtaccac cgcgcatggt cttcggcgct cactgcgcgc ccggatcggc tccggcgcac
     2101 ggcatccgcg tcggagcggc acgcgtgcac gaacgtgcgg tgttccacag cggcgggcac
     2161 cagcaacacc tcgccccggg tgacgacctg ctgcgcgccg acgatcttgt acgcgtcgcg
     2221 ccacgagaac gcgctgtcgt ccatccgcgc cacgagcgcg cgcacgaact cgaggtcctc
     2281 ggcggtgtcg acggtccaac gctcaccacc gagccactgg tcgttgcgga cattcgcgag
     2341 ccggaagcgc tccggccgcc ggtagacgaa cggcgtcacg tgctcacgct cggcggggtc
     2401 ggtcgcttcc tcgcccgccg tccgcaacac cgaagcgcgc acgacttcga cgtcgagtcc
     2461 cttcgggaag gtccgcggga acacgttgct cgtgtagtcg gcgtcgcact cgaagtgccg
     2521 tgcgacgact gtttccacga gcttcggatc gatcaacggg cagtcggccg tgagccggat
     2581 cacatggtcg gccggcgccg catcgagcgc cgtcacgaac cggcccaaca cgtcggtctc
     2641 ggagccgcgc acgacgcgat agccggcgtc acgcccgata tccgcgacca catcgtcacg
     2701 cgcgagcgtg ctcgtcgcga ccaccagcat atcgacgtga acggtcgcga gccggtcgag
     2761 catgaaccgc aacatgggcc ggtcgtcgac gggctggagc actttgccgg gcaagcgcgt
     2821 cgaacccgtg cgggcctgca ccacgcacgt gacgcgcact agaacgtcct gaccgggttg
     2881 cgcacgtcgg ccgcctgttc gaccagttgg gccgcggcgc gcgcggcgcc ctgcccgtcg
     2941 atcgcttccg cggcggcccg ggcgagcgcg cggcgggcgg cggcgtctcg acgcagtccg
     3001 ctcactgccg tcgcgacctc gtcgtcggca acggcgagca ccgcgccgac gcggtgcagg
     3061 tactcgacgg ccgtgcgctg gttctcggcc atggtgacgg caacgacggc gcgcgcgagg
     3121 cggcaggcct ccaacaacgc gacgccgccg gccgaaacga cgatctgggc accggccatc
     3181 tctccgctca acgacgacgg cgcttgcacg ccgacgacgc cgtccgggat cgactgcgaa
     3241 ccccacggtc cgatcaccag ccgcacctgg aggtcgggct ccgcggtgtg gatggccccg
     3301 gcgaggtccg cgcccgt
//
LOCUS       DASUGA010000144         2840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1432808, whole genome shotgun sequence.
ACCESSION   DASUGA010000144 DASUGA010000000
VERSION     DASUGA010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432808"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VFX21_02785"
     CDS             complement(<1..501)
                     /locus_tag="VFX21_02785"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX5094907.1"
                     /translation="MTLTDEQIAAAALAALPGITPKRLRCLLEAFGSAGAAAHAVGTG
                     DIRAVLHRDPERVERTVADWRSALDLDATRAVLEARRAQVWVERGWGFPIHDGIPDRP
                     AVLLSEGHGVDAFAAPRVGIVGTRSASPQGIADAHELGAFLAAAGVTVVSGLALGIDG
                     AAHEGAL"
     gene            complement(498..1994)
                     /locus_tag="VFX21_02790"
     CDS             complement(498..1994)
                     /locus_tag="VFX21_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX5094908.1"
                     /translation="MLASVRSAVLRGVDGQLVTVEVHVSRGLPGYTVVGLPDAAGRES
                     RERVRAAYMSCGFEWPQKRVTVNLAPADVRKTGSALELAVAVSVMAADELLPADALDG
                     LAILGELGLDGRVRSVPGVLVLIDALAYAGVGRVIVPLANAAEAALVPNIEVRPARTL
                     AELRACLKAECPWPDVPPSPPATNPCDDDPVDLCEVRGLPIAQHALAIAAAGAHHLLF
                     VGSPGVGKTMLARRLPTILPPLARDEALEVTKIRSAAGLGSTGVLCTARPFRAPHHSA
                     TMPALIGGGSPQVRPGEISLSHRGSLFLDELAEFPPPVLDALRQPLEERVVRVSRAAG
                     TFEFPADFLLVACCNPCPCGRPDPKCMCTDMQRARYLRRLSAPLMDRFDLRLEVGASD
                     PDAAAGEESEVVAARVAAALARQQHRYMETPWRRNAHVAGGALPVFMPLDRDVESAWR
                     ALCRTRRLSGRGAARVRRVARTIADLDERPDVTADDIELAGMLREDVR"
     gene            2142..>2840
                     /locus_tag="VFX21_02795"
     CDS             2142..>2840
                     /locus_tag="VFX21_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX5094909.1"
                     /translation="MDHDAAGFAATLTALSPHTRRAYGHDVDEFVAWCERGACVDPAA
                     LEHKTLRRYLAYLQTRGFSRPTIARKAAAVRAYTRYLRRRGVLHRNVAVELRSPKGAR
                     RLPRVPRAAEAGAVLAGAAAEVDEHRARRDLAVLELLYGAGLRVSECCGLDRGDVDLR
                     RRAVTVLGKGSKIRRVPLGEPAADAVAAYLGGARAAWVAGETTDDALFLNARGRRLGP
                     RDVRRICDRYRLPDG"
BASE COUNT          421 a         1033 c         1025 g          361 t
ORIGIN      
        1 gagcgcgcct tcgtgcgcgg cgccgtcgat gccgagggcc aggccgctga cgaccgtcac
       61 gccggcggcc gcgaggaacg cgcccagctc gtgcgcgtcg gcaatgccct gcgggctcgc
      121 ggaacgggta ccgacgatgc cgacccgagg cgcggcgaac gcatcgacgc catgtccttc
      181 gctcagcagc accgcgggcc ggtcggggat gccgtcgtga attgggaagc cccagcctcg
      241 ctcgacccac acctgcgccc gccgggcctc gagcactgcg cgcgtcgcat cgagatcaag
      301 cgcgctccgc cagtcggcga cggtgcgttc gacgcgctcg gggtcacggt gcaagacggc
      361 gcggatgtcg ccggtaccga ccgcgtgcgc ggccgcgccg gccgagccga atgcctccaa
      421 gaggcaacgc agtcgtttgg gtgttatgcc cggcagcgcg gccaacgcgg ccgccgcgat
      481 ctgttcgtcg gtgagcgtca tcgcacgtct tcgcgcagca tgccggccaa ctcgatgtcg
      541 tcggccgtga cgtcgggtcg ttcgtcgagg tcggcgatcg tgcgcgcgac tcgccggacg
      601 cgtgcggcac cgcgtccgct caagcggcgc gtgcgacaca acgcacgcca cgcgctctcg
      661 acgtctcggt cgagcggcat gaacaccggc aacgcgccgc ccgcgacatg ggcgttgcgc
      721 cgccagggcg tctccatata gcggtgctgc tggcgtgcga gcgcggccgc gacacgcgcc
      781 gcgacgacct cggactcctc gcccgcagcc gcgtcgggat ccgacgcgcc tacctcgagc
      841 ctcaggtcaa agcgatccat gagcggcgcc gacagccggc gcaggtaacg cgcacgctgc
      901 atgtcggtgc acatgcactt cggatccggc cgaccgcatg gacacgggtt gcagcacgcg
      961 accaacaaga agtcggccgg gaactcaaac gtgccggcgg cccggctcac ccgcacgacg
     1021 cgctcttcga gcggttggcg taaggcgtcg agcaccggcg gcgggaattc ggccaactca
     1081 tcgaggaaca aggagccccg atgcgacagc gagatctcgc ccggtcgcac ttgcgggctt
     1141 cccccaccga tgagcgcggg catggttgcg ctgtgatgcg gcgcccggaa cggccgggct
     1201 gtacacagga cgccggtgct ccccaggccc gcggccgacc ggatcttggt gacctcgagc
     1261 gcttcgtcgc gagcgagcgg cggcaggatc gttggcaatc gccgcgccag cattgtcttg
     1321 ccgacgcccg gcgacccgac gaacagcagg tggtgcgcgc ccgcggccgc gatcgccaat
     1381 gcgtgctgcg caatcggcag cccgcgcacc tcgcacaggt cgaccgggtc gtcgtcacac
     1441 gggttcgtgg cgggcggtga cggtggcacg tctggccacg ggcattcggc cttcaagcac
     1501 gcgcgcagct cggcgagcgt gcgcgccggc cgcacttcga tgttgggcac gagggcggcc
     1561 tcggccgcgt tcgccagggg aacgatgacg cgcccgactc cggcgtacgc gagcgcatcg
     1621 atcagcacca acacgccggg cacggaccgg acgcgcccgt cgagaccgag ctcgcccaag
     1681 atcgcgaggc cgtcgagcgc gtcggccgga aggagctcgt cggccgccat caccgagacc
     1741 gccaccgcga gctcgagcgc cgaacccgtc ttgcgcacat ccgcgggcgc aaggttcacg
     1801 gtgacgcgct tctgtggcca ctcgaagcca cacgacatgt acgcggcgcg gactcgctcg
     1861 cgtgactcgc gcccggccgc gtcgggcaac ccgacgacgg tgtaaccggg gagcccgcgc
     1921 gacacgtgca cttcgacggt gacgagctgc ccgtcgactc cccgcaacac ggcgctgcgc
     1981 acagatgcca gcacgacgac ctccttcgaa cgaacaggga aggagggtcg gataccggac
     2041 ctcgacggtg cgatgaaccg tacgcggcgg gtgtgacaac gacgagtgac gtggactggc
     2101 atgaacgaac gccctggtcg gggcggctac cgtcgtgcgg aatggaccac gacgcggcgg
     2161 gctttgcggc gacgctgacc gccctcagcc cgcacacgcg gcgcgcgtac ggccacgacg
     2221 tcgacgagtt cgtcgcgtgg tgcgagcgcg gcgcatgcgt cgatcccgcc gcgctcgagc
     2281 acaagacgct gcgccgctac ctcgcgtatc tccagacgcg cggcttctcg cgtccgacga
     2341 tcgcccgcaa ggccgccgcg gtacgggcct acacccgtta cctgcggcgc cggggcgtgc
     2401 tgcaccgcaa cgtcgcggtc gagctccgca gccccaaagg cgcgcgccgc ctgccgcggg
     2461 tgccccgggc ggccgaagcc ggcgcggtgc tcgcgggcgc agccgcggag gtcgacgagc
     2521 atcgggcccg ccgcgacctc gcggtgctcg agctgctcta cggcgctggg ctacgagtca
     2581 gcgagtgctg cggcctcgac cggggtgacg tcgatctgcg ccggcgggcc gtcaccgtgc
     2641 tgggcaaggg ctcgaagatc cgccgggtgc cgctgggcga gccggccgcg gacgcggtcg
     2701 cggcctacct cgggggcgcg cgggcagcgt gggtggcggg ggagacgacc gacgacgccc
     2761 tgttcctcaa cgcccgcggg cggcgcctcg ggccccgcga cgttcgccgg atttgtgacc
     2821 gttaccggct cccggacggc
//
LOCUS       DASUGA010000145         2592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1573879, whole genome shotgun sequence.
ACCESSION   DASUGA010000145 DASUGA010000000
VERSION     DASUGA010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573879"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..1427
                     /locus_tag="VFX21_02800"
     CDS             273..1427
                     /locus_tag="VFX21_02800"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5094910.1"
                     /translation="MNLKTAARRLGVHYQTAYRWVRTGQLVAVKVGAGYEISDAALER
                     FQAQRAALERIPDVTPRTTPMVPSARVDALNTLDSMLRVTTVDAGAVCNRAVDVVANV
                     VGDGAVLQLSRTDRSDATLAFHHYDAERSVLMGAVLQLGRTDAGSFAGRAAASDAALL
                     VPQVPQREVRSMLAPEFHQVLGDVGFYSAISAPISVSGQFRGTLFAGRDTPGRPYTVE
                     DRDFVVAVASRVALASTRADRGRQAWELRSRIMSELAEHGGYSDAIDRAAAWIANGSL
                     EHDAAVALVNLDRDVVTLTKPFAAMVGTSATDVVGTPIESLVAQTGDLESTFERLANG
                     ELDYCSVITQAAVTSTAIVMDGAILRGPDATPCCVVYVAHERPEPAAGDL"
     gene            1462..>2592
                     /locus_tag="VFX21_02805"
     CDS             1462..>2592
                     /locus_tag="VFX21_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX5094911.1"
                     /translation="MQDWGHGTELDDVRGEVAIAGIGETAYTKASGRTAREIGAEAAE
                     RAIADAGLRPEDIDGLTWSGAFPDFDAAAFHEHFGTSHDLWTSPWGGGMAWAATAPYL
                     AAQAIREKQARHVLNVFPVAWATQRSSMTGGPGEVHASQSSKQNLEVPFGWFPQPVYF
                     ATIMRRHMLEFGTTDAQFGAIALAARRHANLHPDAVMHGRPMTLDDYLGAAMLADPLR
                     LFDSCLISDGGAAYVTTSVERARDLAQPPVVVAGVGEGYSATGTHWAQQRAFTSTPQV
                     FSAPGAFTMAGCTPADVDVLTVYDPFTVVSMMQIEDMGFCAKGEAGSFVEGDTLHHDA
                     GKLPFNTHGGLLSHAYVLGIAHVVECVKQLRGVAPAQVPDCDV"
BASE COUNT          392 a          884 c          909 g          407 t
ORIGIN      
        1 gcatttgggg tcaatcgagg gggatttgtg gggtcaggcc gacagggacg gcggtcgtgt
       61 tcatcgaaca ggagcgtcct cgaccgtacc gcgcgaccgc gatcgtggtc agatgggcga
      121 ttcttcgccc cgaaacgtgc cgcaataggc gacttgctaa aggcacgaga acgcttcgct
      181 cacactcggg agtgccgcag tgtgctcgga tggcgttcac gcaacgcgga ggcgggtccg
      241 cgtgacccat ttccgggcgt ggagaagaac gcgtgaacct caagaccgca gcgcggcgtt
      301 tgggcgtcca ctaccaaacg gcctaccgct gggtccgcac ggggcagctg gtcgcggtca
      361 aggtcggcgc gggctacgag atcagcgacg cggcgctcga acgcttccag gcgcagcggg
      421 ccgcgctcga acgcatcccg gacgtcacac cccgcacgac gccgatggtg ccgagcgcgc
      481 gcgtcgacgc cctgaacacc ctcgactcga tgttgcgcgt gacgacggtc gacgccggtg
      541 ccgtctgcaa tcgcgccgtc gacgtcgtcg cgaacgtcgt cggcgacggc gcggtgttgc
      601 agttgtcgag aaccgaccgc tcggacgcca cgctcgcgtt ccaccactac gacgccgaac
      661 ggtcggtgct catgggcgcg gtcttgcagc tcggccgcac cgacgccggc tccttcgcgg
      721 ggcgggcggc cgccagcgac gcggccttgc tggtgcccca agtgccccag cgtgaagtcc
      781 ggtcgatgtt ggcgccggag ttccatcagg tgctcggcga cgtcgggttc tacagcgcga
      841 tctccgcgcc catctcggtc tcgggccagt tccgcggaac gctgttcgcc ggtcgcgaca
      901 cgcccggacg cccgtacacc gtcgaagacc gcgacttcgt cgtcgcggtg gcgtcccggg
      961 tcgcgctggc gtcgacccgc gccgatcgcg gacgccaggc ctgggagctg cgctcgcgca
     1021 tcatgagtga gctcgccgag cacggcggct acagcgacgc gatcgaccgg gccgcggcgt
     1081 ggatcgcgaa cggctcgctc gaacacgatg ccgcggttgc gctcgtcaat ctcgaccggg
     1141 atgtcgtcac gttgaccaag ccgttcgcgg caatggtggg aacgtctgcg accgacgtcg
     1201 tcggaacgcc gatcgagtcg ctcgtcgcgc agaccggcga tctcgagtcg acgttcgaac
     1261 gcctggcgaa cggcgagctc gactactgct cggtcatcac gcaggcggcg gtgacgtcga
     1321 ccgcgatcgt gatggacggc gcgatcctcc ggggtccgga cgcgacgccc tgttgcgtcg
     1381 tgtacgtggc gcacgagcgt ccggagcctg ccgccggcga cctttagttc accggacggg
     1441 ccgctggtac gttccccgtc cgtgcaggac tgggggcacg gcaccgagct cgacgacgtg
     1501 cgcggcgagg tcgcgatcgc gggcatcggt gagaccgcgt acacgaaggc atccggccgc
     1561 accgcgcgcg agatcggcgc ggaggccgcc gaacgtgcca tcgccgatgc cggcctgcgg
     1621 ccggaggaca tcgacggcct cacgtggtcc ggtgcgttcc cggacttcga cgctgccgcc
     1681 ttccacgagc acttcggcac cagccacgac ttgtggacgt cgccgtgggg cgggggcatg
     1741 gcgtgggccg cgaccgcgcc gtacctcgcg gcgcaggcca tccgcgagaa acaggcgcgc
     1801 cacgtcctga acgtgttccc ggttgcgtgg gcgacgcaac gttcctccat gaccggcgga
     1861 ccgggtgagg tgcacgcgtc gcagtcgtcg aagcagaacc tcgaagtgcc gttcgggtgg
     1921 tttccgcagc ccgtgtactt cgccacgatc atgcgccggc acatgctcga gttcggcacg
     1981 accgacgcgc agttcggtgc gatcgcgttg gccgcgcgac ggcacgccaa cctccatccg
     2041 gacgcagtca tgcacggccg accgatgacg ctcgacgact acctcggcgc cgcgatgctt
     2101 gccgacccgt tgcggctgtt cgactcgtgc ctcatctccg acggcggcgc ggcgtacgtg
     2161 acgacgagcg tcgagcgcgc ccgcgacctc gcgcagccgc cggtggtggt tgccggtgtc
     2221 ggtgagggct actcggcgac cggtacgcat tgggcgcaac aacgcgcgtt cacgagcacc
     2281 ccgcaggtgt tctcggcgcc cggcgcgttc acgatggcgg gatgcacgcc cgcggacgtc
     2341 gacgtgctga ccgtctacga cccgttcacg gtggtcagca tgatgcagat cgaggacatg
     2401 ggcttctgcg cgaaaggtga agccggttcg ttcgtcgaag gcgacacgtt gcaccacgac
     2461 gccgggaaac tgccgttcaa cacacatggc ggactgctgt cgcacgccta cgtgctcggt
     2521 attgcgcacg tcgtcgagtg cgtcaagcaa ctgcgcggcg tcgctcccgc gcaggtcccg
     2581 gattgcgacg tc
//
LOCUS       DASUGA010000146         2489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_479964, whole genome shotgun sequence.
ACCESSION   DASUGA010000146 DASUGA010000000
VERSION     DASUGA010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479964"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VFX21_02810"
     CDS             complement(<1..737)
                     /locus_tag="VFX21_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094912.1"
                     /translation="MTVEIETTRVDHARQFARKLARNARDRNEWRKIVRFRLWMPVVL
                     QCLLIVALLAYTEHRFDGFVNAANITQILLLAMPLALVSIAQTHALLVGYLDLSVGSM
                     ISFGVVVGSFRIGADASTFGILSGVALVLVCGIALGLVNALLIRGFRIPSLIATLATL
                     SILDGISLTMRPTAQGVISDDLVSFLRTSVGAIPIAFIVIVVGAGALDVWLHGSGSGL
                     KVRAVGFDDRAAKRGGIRNDSIRVRALV"
     gene            complement(734..2311)
                     /locus_tag="VFX21_02815"
     CDS             complement(734..2311)
                     /locus_tag="VFX21_02815"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX5094913.1"
                     /translation="MSSELVLELAGVSRQFGAVRALRNVSFDCRAGEVHALVGENGSG
                     KSTLLGVASGFVDPDRGTVRIDGKPLTRDSPALSRKLGLAMAYQDGSSVLAEPVKNNL
                     YSAAAASGRPPYWRRKKWARHVLAEFDLDLELFPDAPVGSLTMAERQLFEVAKALVNA
                     PKVLLLDEPTTALGPDEVQALHRTVLACKRQGIGVVYVSHRLPEVLEVADRITVLRDG
                     QYQGTFDAATTTEPQLVEHIVGRPFDAVFPQRQTSAADAEEVLVVDALQGQSFGPVSF
                     TLRRGEVAGIAGAEGNGQSHLFDSLAGRTPPRAGRVTCRGKDLSLISAREAVAAGIML
                     LPGDRKHEALMGVLGVRVNATVQSLRRFSRLGVLRRRAERDAVQQLIEQLEIRTPWLD
                     QPVEFLSGGNQQKVSVARSFLREPAVILAYEPTQGVDVGSRVDIYQALRARAADGAAL
                     LVKSSDPIELSGLCDRVFVMSRGTIVEEIPGDELDETRIVEAIVRGPGMSKAARSPLG
                     VAMPKAPSATRSADEIA"
BASE COUNT          426 a          846 c          854 g          363 t
ORIGIN      
        1 accaaggcac ggacccggat cgagtcgttc ctgatgccgc cccgcttggc ggcacgatcg
       61 tcgaacccga cggcccgcac cttgagcccc gagcccgacc cgtgcagcca gacgtcgagc
      121 gcgccggccc ccacgacgat cacgatgaag gcgatcggga tcgcccccac gctggtgcgc
      181 aggaacgaca ccaggtcgtc gctgatgacg ccctgggcgg tcggtcgcat cgtgagcgag
      241 atgccgtcga ggatgctgag cgtcgccagc gtggcgatca acgaggggat gcggaacccg
      301 cggatcagca aggcgttgac caacccgagg gcgatgccgc acaccaacac gagcgcgaca
      361 ccgctgagga tgccgaacgt cgaggcatcg gcaccgatcc ggaacgaccc gacgacgacg
      421 ccgaagctga tcatcgagcc gacggagaga tcgagatagc cgacgagcag ggcatgggtc
      481 tgggcgatgc tgaccagcgc cagtggcatc gccagcagca ggatctgggt gatgttggcc
      541 gcgttgacga acccgtcgaa acggtgctcg gtgtaggcca gcagggcgac gatcaagagg
      601 cactgcagca cgaccggcat ccacagccgg aacctgacga tcttgcgcca ctcgttcctg
      661 tcgcgcgcgt tgcgcgccag cttgcgggcg aactgacggg cgtggtcgac gcgcgtggtc
      721 tcgatctcga cggtcatgcg atctcgtccg ctgagcgagt tgcggatggc gccttcggca
      781 tggcgacgcc gagcggcgaa cgcgcggcct tcgacatccc gggaccgcga acgatggcct
      841 cgacgatgcg ggtctcgtcc aactcgtcgc cggggatctc ctcgacgatc gtgcctcgcg
      901 acatcacgaa cacccggtcg cacaatcccg agagctcgat cggatcgctc gacttgacga
      961 gcagtgctgc gccgtcggcc gcacgtgccc gcaacgcttg gtagatgtcg acgcgcgaac
     1021 cgacgtcgac gccctgcgtc ggctcgtacg ccaagatgac cgcaggctcg cgcaggaacg
     1081 agcgggcgac ggacaccttc tgctggttgc cgcccgacaa gaactcgacg ggctggtcca
     1141 gccaaggggt gcggatctcg agctgctcga tgagctgttg caccgcgtca cgctcggcgc
     1201 gcctgcgaag cacgccgagc ctgctgaagc ggcgcaacga ctgaacggtg gcgttgaccc
     1261 gcacgccgag cacacccatc aacgcctcat gcttgcggtc gccgggaagc agcatgatcc
     1321 ccgcggcgac agcctctcgt gccgagatca ggctgagatc cttgccgcgg cacgtgactc
     1381 ttccggcgcg aggcggcgtg cgtcccgcca gcgagtcgaa caggtgcgac tggccgttgc
     1441 cctcggcacc ggcgatgccc gcgacctcac cgcgcctgag cgtgaagctg acgggaccga
     1501 acgactggcc ctgcagcgca tcgacgacga gcacctcctc ggcgtcggct gccgaggtct
     1561 ggcgctgcgg gaagaccgca tcgaacggcc gcccgacgat gtgctcgacg agctgcggtt
     1621 ccgtcgtcgt ggcagcgtcg aaggttccct ggtactgacc gtcgcggagc acggtgatcc
     1681 ggtcggcgac ctcgagcacc tcgggcagcc ggtggctgac gtacacgacg ccgattcctt
     1741 gacgcttgca ggccaacacc gtgcggtgga gggcctgcac ctcgtcgggt ccgagcgcgg
     1801 tggtgggctc gtcgagaagc agcaccttgg gtgcgttgac gagtgccttg gcgacctcga
     1861 acagctggcg ttcggccatc gtcagtgatc cgaccggggc gtcggggaag agctcgaggt
     1921 cgaggtcgaa ctcggccagc acgtggcgcg cccacttctt acgccgccag tacggcggtc
     1981 gtcccgacgc cgccgccgcc gaatacaggt tgttcttgac cggctcggcg agcaccgacg
     2041 atccgtcctg atacgccatc gccagaccga gcttgcgcga gagcgcgggc gagtcgcggg
     2101 tgagtggctt gccgtcgatc cggacagtgc cgcggtccgg atcgacgaac cctgatgcaa
     2161 ccccgagcag cgtcgacttg ccggagccgt tctcgccgac gagcgcgtgc acctcgccgg
     2221 cgcgacagtc gaagctgacg ttgcgaagcg cgcggacggc gccgaactgg cgggacacgc
     2281 ccgccagttc gagcaccaat tcggaactca tcgatgctgc cgcgtgatcc ctcggatcat
     2341 ccggcgaaca tctcggcgag cacgctgtcc ggggcgagcg acgtacccga caccacctcg
     2401 ttgacgcggt cggggttgca gtcggcggcg gtgacctgcc gcatgacgtg cggcaccgtg
     2461 acctcgccgg ggatctcggc gccctgcag
//
LOCUS       DASUGA010000147         3714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1849922, whole genome shotgun sequence.
ACCESSION   DASUGA010000147 DASUGA010000000
VERSION     DASUGA010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849922"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VFX21_02820"
     CDS             complement(<1..951)
                     /locus_tag="VFX21_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5094914.1"
                     /translation="MTARPEPRDDLKALSGYHSPQVDVGVRLNTNESPYAPPPAFVQR
                     WLAALGDVDWNRYPDRDARALKDALGAFLGQPANRLLCGNGSNEVLQTLLLAYGGAGR
                     KALMFEPTYALHAQIARGTGTEVIAGERRADYSIDPDDAVALIAREKPSVVFVCSPNN
                     PTGTVEPPETIARLAEAAAAWGAVLVVDEAYGEFAPWSAISLVDDESPIAVVRTYSKV
                     WSMAGVRLGFGVAPSWMIDELEKVLLPYNLSVPTQIAGTVALDFKAEMEQRVASLVEE
                     RGRVFSALAQTGGIDVCPSGANFLLFRVAGDAHDLWKRMLD"
     gene            complement(948..2231)
                     /gene="hisD"
                     /locus_tag="VFX21_02825"
     CDS             complement(948..2231)
                     /gene="hisD"
                     /locus_tag="VFX21_02825"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX5094915.1"
                     /translation="MLTRIDLRGVPGDLRTLLATEDAGPDVAPAVGEIIASVRAGGDA
                     ELRKLTARFDGVDLDDVFVSPGAIAEALEQISPGLRAALELARDQILAWHEAQREKEA
                     RHERLGIEVHEMVIPVDRAGCYVPGGRAQYPSSVLMTAIPARVAGVPEVILCVPPGPD
                     GAVASSVLAAAAIAGVDAVYRVGGVQAIAAMAYGTESIRPVDVIVGPGNAYVAEAKRQ
                     VFGTVGIDGLAGPSEVAIVADDTVDAMWVAADLLAQAEHGPGGAVTVIVWDDDVADRV
                     DLALETLLVTTPRSDEAQATLESGGRVVFVADPPAAVEAANAIAPEHLQLMCADAELL
                     VPLVRHAGAVFVGPYAPAVLGDYVAGTNHVLPTGGTARFASALRVADFQKHVHVVGAD
                     AAAVERVGPATRILAEEEGLFAHADALRLREEGKP"
     gene            2303..2509
                     /locus_tag="VFX21_02830"
     CDS             2303..2509
                     /locus_tag="VFX21_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094916.1"
                     /translation="MTASSDLAVLSSVVSQVEDLSRRVTELAERYGETPDSAVAAELF
                     AGEGALATARRTLERAATLLQTLA"
     gene            complement(2539..3183)
                     /gene="nth"
                     /locus_tag="VFX21_02835"
     CDS             complement(2539..3183)
                     /gene="nth"
                     /locus_tag="VFX21_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012626829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX5094917.1"
                     /translation="MARPRGAKVRALVVVDRLKEMYPDAQCALKHTNAYELLAATILS
                     AQTTDERVNMVTPALFARYPTPEDLAGADIEELEGIIQSTGFFHSKAKNLIGMAQGVV
                     ARFGGEIPVALDDFVTLPGVGRKTGNVVRSVWYGEPGLPVDTHVTRLSARLKLTNETD
                     AVKIEKDLGGIVPPEEWGMLSLRLIEHGRQVCIARKPKCDTCALNDICPSAFKV"
     gene            3207..>3714
                     /locus_tag="VFX21_02840"
     CDS             3207..>3714
                     /locus_tag="VFX21_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018511155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094918.1"
                     /translation="MPHPSVESFGPAEFGRIIDRVRALDRQDLNEAVWRDLEHPQPDS
                     AVYLLDDAAVAHVARSDNFAPQHWALGVAGAPRVPVLQAALDHVAHHDGGRAALWVLG
                     ATDADDEELVQAGLLPDRELHEMRVPLPIAEEPRFKPGIDVRTFEPGRDEEAWLVVNN
                     RAFANHAEQ"
BASE COUNT          583 a         1324 c         1253 g          554 t
ORIGIN      
        1 gtcgagcatc cgtttccaca gatcgtgggc atcgcccgcg acccggaaca gcaagaagtt
       61 ggcgcccgat ggacacacgt cgatgccgcc ggtctgcgcc agtgcagaga agacgcgtcc
      121 ccgttcctcg acgagcgacg cgacacgctg ctccatctca gccttgaagt cgagcgcgac
      181 cgtgccggca atctgggtgg gaaccgacag gttgtacggc agcagcacct tctcgagctc
      241 gtcgatcatc cacgacgggg cgacgccgaa gccgagacgc acgccggcca tcgaccacac
      301 tttcgagtag gtgcgcacga ccgcgatcgg agattcgtcg tcgacgagcg agatcgcact
      361 ccacggcgcg aactcgccgt aggcctcgtc gacgacgagc accgcacccc acgccgcggc
      421 cgcttccgcc aggcgcgcga tcgtctcggg cggttcgacg gtgccggtcg ggttgttcgg
      481 gctgcagacg aacacgaccg acggcttctc gcgcgcaatc aacgcgaccg cgtcgtcggg
      541 gtcgatcgag tagtcggcgc gccgctcccc cgcgatgacc tcggtaccgg tcccgcgtgc
      601 aatctgcgcg tgcaacgcgt aggtcggctc gaacatcaaa gccttgcggc cggcaccgcc
      661 gtacgcgagc agcagcgtct gcaggacctc gttcgagccg ttgccgcaca gcagccggtt
      721 cgcaggctgg ccgaggaacg cgccgagcgc atccttcagc gcacgcgcgt cgcggtcggg
      781 ataccggttc cagtcgacgt cgccgagcgc cgcgagccac cgctgcacga acgcgggcgg
      841 tggcgcgtac ggactctcgt tggtgttcag gcgcacgccg acgtcgacct gcggcgagtg
      901 gtagccggaa agcgccttca ggtcgtcgcg cggctcggga cgcgcggtca cggcttgcct
      961 tcttcccgca agcgcagcgc gtcggcgtgc gcgaacaagc cttcttcctc ggcgagaatc
     1021 cgggtggccg gtccgactcg ttcgaccgct gccgcgtcgg cccccacgac gtgcacgtgc
     1081 ttttggaagt cagcaacgcg taacgcgctg gcgaagcggg cagtaccgcc ggtcggcaac
     1141 acgtggttcg ttcccgcgac gtagtcgccg aggaccgcgg gtgcgtaggg gccgacgaag
     1201 accgcgcccg cgtgccgcac gagcggcacg agcaactcgg cgtcggcaca catcaactgc
     1261 aggtgctcag gtgcaatcgc gttcgcggcc tcgacggccg ccggcggatc ggcgacgaac
     1321 acgacccggc cgcccgattc gagcgtcgcc tgggcctcgt cgctgcgcgg cgtggtcacc
     1381 agcaacgttt cgagtgcgag atcgacgcgg tcggcgacgt cgtcgtccca cacgatgacg
     1441 gtcaccgcgc cgccgggccc gtgttcggcc tgcgcgagca aatcagctgc gacccacatc
     1501 gcgtcgacgg tgtcgtcggc aacgattgca acctcggacg gaccggcgag gccgtcgatc
     1561 ccgaccgtgc cgaacacttg acgtttggct tccgcgacgt acgcgttgcc cggcccgacg
     1621 atgacgtcga ccgggcggat cgactcggtg ccgtacgcca tcgccgcgat ggcctgcacg
     1681 ccgccgacgc ggtacacggc gtcgacgccg gcgatggcag ccgccgcgag gaccgacgac
     1741 gcaaccgcgc catcgggtcc gggcggcacg cagaggatca cttcggggac gcccgccacg
     1801 cgcgcgggaa ttgcggtcat cagcaccgac gacgggtact gggcccgccc gcccgggacg
     1861 tagcaaccgg cccggtcgac cggaatcacc atttcgtgga cttcgatccc cagccgctcg
     1921 tggcgcgctt ccttctcgcg ctgcgcctcg tgccaggcga gaatctggtc gcgcgccaac
     1981 tcgagcgcgg cgcgcaggcc cggcgagatc tgttcgagtg cctcggcgat ggccccgggc
     2041 gagacgaaga cgtcgtccag gtcgacgccg tcgaagcgcg ctgtcagctt ccggagctcg
     2101 gcgtcgcccc cggcacggac cgaggcgatg atctcgccga ccgcgggcgc gacgtcaggg
     2161 cccgcatcct cggtcgcgag gagtgtgcgg aggtcgccgg gaacgccgcg aaggtcgatg
     2221 cgcgtgagca tggagaacgg ctcatcgtac cggccgtccc ccggaggctt gatccgaatt
     2281 gccctgtggc acactgcgcg acatgaccgc gagcagcgac ctggccgtcc tctccagcgt
     2341 ggtgtcccag gtcgaggacc tcagccggcg cgtcaccgaa ctggccgagc gctacggcga
     2401 gacgcccgac tcggcggtcg cggccgagct cttcgcgggc gaaggcgccc tcgcgaccgc
     2461 ccgccgcacc ctcgaacgcg ccgccaccct cctccaaacc ctcgcctaac cgcgtacctg
     2521 cgtccgcagg ttgcgttgtt agaccttgaa ggcgctcggg cagatgtcgt tgagtgcgca
     2581 ggtgtcgcac ttcggcttgc gcgcgatgca gacctggcgg ccgtgctcga tgaggcggag
     2641 cgacaacatg ccccattctt cgggcgggac gatgccaccg agatccttct cgatcttgac
     2701 cgcgtcggtc tcgttcgtga gcttgagccg cgcggacaat cgcgtcacat gcgtgtcgac
     2761 cggcaatccc ggttcgccgt accaaaccga gcggaccacg ttcccggtct tgcggccgac
     2821 gccgggcagt gtgacgaagt cgtcgagcgc caccgggatc tcaccgccga atcgcgcgac
     2881 gacgccctgc gccatgccga tgagattctt cgccttcgag tggaagaagc ccgtcgactg
     2941 gatgatgcct tcgagctcct cgatgtcggc accggccaaa tcctcgggcg tcgggtaccg
     3001 ggcgaacaat gccggcgtga ccatgttgac gcgctcgtcg gtggtctgcg ccgaaaggat
     3061 cgttgcggcg agcaactcgt aggcgtttgt gtgcttgagc gcgcattgcg cgtcgggata
     3121 catctccttc agccggtcga caacgaccag cgcccggacc tttgcaccgc ggggacgcgc
     3181 cacgggcgca cggtaccctc ccgtgcgtgc cgcacccgtc tgtggagagc ttcggcccgg
     3241 ccgaattcgg acgcatcatc gatcgcgtcc gcgcgctcga ccgtcaggat ctcaacgaag
     3301 ccgtgtggcg cgatctcgag cacccgcaac cggactcggc cgtctacctg ctcgatgacg
     3361 ccgctgtcgc gcacgtcgcg cgcagcgaca acttcgcgcc gcagcactgg gccctcggcg
     3421 tcgcgggtgc gccgcgcgtg ccggtcctgc aggcggcgct cgaccacgtc gcacaccacg
     3481 acggcggccg cgctgcgctt tgggtgctcg gcgcgaccga cgccgacgac gaggagctgg
     3541 tgcaagccgg tcttctcccc gaccgcgaac tccacgagat gcgggtcccg ttgcccatcg
     3601 ccgaagaacc gcggttcaaa ccggggatcg acgtgcgcac cttcgaaccg ggccgcgacg
     3661 aggaggcgtg gctcgtcgtc aacaaccgcg cgttcgcgaa ccacgcggaa cagg
//
LOCUS       DASUGA010000148         2054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1432295, whole genome shotgun sequence.
ACCESSION   DASUGA010000148 DASUGA010000000
VERSION     DASUGA010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432295"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VFX21_02845"
     CDS             complement(<1..523)
                     /locus_tag="VFX21_02845"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX5094919.1"
                     /translation="MTDELATFDDARTMRYTRFFAHPIEDVWDAVTEAEQLDVWLSPA
                     CGVLVERRVGGRCVFGFGSPIADGFEGTVTEYDPPRLVHYQLEGSSLRFELEAVDGGT
                     RLDFIHAFKPGIVSPAFDPAELPAGPGTPWHPDFCAGFHEMIDQLGWMLAGTWTAADA
                     KPFVDAVYHGGDVY"
     gene            complement(520..837)
                     /locus_tag="VFX21_02850"
     CDS             complement(520..837)
                     /locus_tag="VFX21_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5094920.1"
                     /translation="MQVFEALADPTRQRIVELLAARDLSAGDIAAHFDVSRPAVSRHL
                     RVLREAGLTSVTRDAQQWVYRLDTKPLRDMETWLLKTRAHFDARLDALGAHLDRRAKE
                     RQR"
     gene            908..>2054
                     /locus_tag="VFX21_02855"
     CDS             908..>2054
                     /locus_tag="VFX21_02855"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5094921.1"
                     /translation="MPEGLLVGLRVLDLADDPGARAARILGDLGAAVIRVVPADGDPL
                     HGNVARAWNAGKDVRVLGADAPELDELLASADVVFDTPFARGVHVLDPGRAPHAVWVS
                     ITPFGRTGPRESWRASDLGVMAASGNMFCTGDPDRAPIRCTEPTGYGHTGPEAALAAL
                     TALWTDVPHRVDVSMQEVVIISNMAGPAPFPKTGFRGQRRGANIGRTREIWPTKDGFV
                     SFGLRGGKARVASLELIAKLADLPPRDWSQFNQNTATDEELKDLEERLATYFSQQTMQ
                     ELYDIACETNLMLAPANSPREILNSAQLAARDFFGPVDGVERFPRSFVVARSADGEVA
                     PARPRAGAAPWRSGGGRSTAAPTGRKAWDGLKLLEFGSGAAGPIATRY"
BASE COUNT          306 a          704 c          730 g          314 t
ORIGIN      
        1 catagacgtc accgccgtgg tacaccgcgt cgacgaacgg cttcgcgtcg gcggccgtcc
       61 acgtccctgc gagcatccaa ccgagttggt cgatcatctc gtggaagccg gcgcagaagt
      121 cggggtgcca cggcgtgccc ggaccggccg gcagctcggc cggatcgaac gcggggctga
      181 cgattccggg cttgaacgcg tggatgaaat cgaggcgcgt cccgccgtcc acggcttcga
      241 gctcgaagcg cagtgagctg ccttcgagtt ggtagtgcac gaggcgcggc gggtcgtact
      301 cggtgaccgt gccttcgaac ccgtcggcga tcgggctgcc gaacccgaag acgcaacgtc
      361 cgccgacgcg ccgctcgacg agcacaccgc acgcgggtga gagccagacg tcgagttgct
      421 cggcttcggt caccgcgtcc cagacgtcct cgatcgggtg cgcgaagaag cgcgtgtagc
      481 gcatcgtgcg cgcgtcgtcg aaggttgcga gttcgtcggt catctctgtc gttccttcgc
      541 tcgtcggtcg aggtgggcgc cgagggcatc gaggcgcgcg tcgaagtgtg cgcgcgtctt
      601 cagcaaccac gtctccatgt cgcgcagggg cttggtgtcg aggcggtaca cccactgttg
      661 cgcgtcgcgt gtcacgctcg tcagccccgc ttcccggagg acgcgcagat ggcgtgatac
      721 cgcgggacgc gagacgtcga agtgggcggc gatgtcgccg gcactcagat cgcgcgccgc
      781 gaggagctcg acgatgcgct gacgagtcgg gtcggcgagt gcctcgaaga cctgcacccg
      841 gactttgtaa catcacagtt acgtaacgtc aaggttacgc atgtggccgg gcccgtatgc
      901 tgcggccatg ccggaagggt tactcgttgg tctgcgcgtg ctcgacctcg ccgacgatcc
      961 gggggcgcgg gcggcgcgca tcctcgggga cctcggggca gcggtcatcc gggtcgtccc
     1021 ggccgacggc gatcccctgc acgggaacgt cgcccgggcg tggaacgcag gcaaggacgt
     1081 ccgtgtgctc ggcgccgacg cacccgagct cgacgaactg ctcgcgtccg ccgacgtcgt
     1141 gttcgatacg cccttcgcgc gcggcgtgca cgtgctcgat cccggccggg cgccgcacgc
     1201 ggtgtgggtg agcatcacac cgttcggccg caccggaccc cgcgagtcgt ggcgggcatc
     1261 ggatctcggc gtgatggccg cgtcggggaa catgttctgc accggtgatc ccgaccgcgc
     1321 accgatccgg tgcaccgagc cgaccggcta cgggcacacc gggccggaag ccgcgctcgc
     1381 ggcactcact gcgctgtgga ccgatgtccc gcaccgcgtc gacgtctcga tgcaagaggt
     1441 cgtcatcatc tcgaacatgg cggggcccgc gccgttcccc aagaccggat tccggggcca
     1501 acgccgcggc gccaacatcg ggcgcacgcg cgagatctgg ccgaccaagg acgggttcgt
     1561 gtcgttcggg ctgcgcggtg gcaaggcccg cgtcgcgagc ctcgagctga tcgcgaagct
     1621 cgcggacctg ccgccgcgcg actggtcgca attcaaccag aacaccgcga ccgacgaaga
     1681 gctcaaggac ctcgaagaac ggttggcgac atatttctcg cagcagacga tgcaggagct
     1741 gtacgacatc gcctgcgaga cgaacctcat gctcgccccc gcgaactcgc cgcgcgagat
     1801 cctcaactcc gcgcagctcg cggcgcgcga cttcttcggg ccggtcgacg gcgtcgagcg
     1861 cttcccgcgt tcgttcgtgg tcgcccgttc ggccgacggc gaagtcgcgc ccgcgcgccc
     1921 gcgtgcggga gcggcgccgt ggcgctcggg aggcggtcgg tccaccgcgg caccgacggg
     1981 ccgcaaggcg tgggacggcc tcaagctgct cgagttcggc tcgggcgcgg ccggcccgat
     2041 cgcgacgcgg tact
//
LOCUS       DASUGA010000149         4938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1311381, whole genome shotgun sequence.
ACCESSION   DASUGA010000149 DASUGA010000000
VERSION     DASUGA010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311381"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /locus_tag="VFX21_02860"
     CDS             complement(<1..1308)
                     /locus_tag="VFX21_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094922.1"
                     /translation="MGIVEVRVHGVSGTSPDQLLDDPHPRLITGDALSGFYRPRAGRQ
                     PDDRTDHEAYAWGGLTSGSPWRALWVLLAPFAMVNAAASMHRTRARVARPLTRLLALS
                     LTATLVLSTYVLSADLFAWQCGSDTACIERHSYTRFLGWEWLDSPGRRLVVALLLPIA
                     VVCLLWALGRATWQRAEAYVPEPTVMPRVVQTPFDHPQLWDGRARGIFLRHIHISGAT
                     ATLAALLSYALRETTGSMGFELVVAVVLLVVIVALAIPEVPAHVDHRRQIASWVVLAL
                     SWLTLGRVLLFAWTEPTMHSPLPEQLPGTDRTVTYLFVAQLVAILALLAFSRWPAAAV
                     SFVALSLGAAFGAGLVIGVGVLLGDLGTRELRLPLGVSWAARVAALLAAFVAFLVLVY
                     AAVLTARRFGGRPPLPPLERARLAARRTDRVPVYTAVVMAVLVP"
     gene            complement(1345..1551)
                     /locus_tag="VFX21_02865"
     CDS             complement(1345..1551)
                     /locus_tag="VFX21_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094923.1"
                     /translation="MTDTMNLERVRDEQYRRIMDVVVAGPRHRATALARDHLAAFPDD
                     DGVRLVLERLEGRTRPRAHLRLAG"
     gene            1647..2807
                     /locus_tag="VFX21_02870"
     CDS             1647..2807
                     /locus_tag="VFX21_02870"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003956822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5094924.1"
                     /translation="MIANGWVKPGFEGVRDAFAANLAEGRDIGAAFSAYHRGEKVVDL
                     WGGIADPATERPWAEDTLTLVFSTTKGATAICAHRLAQDGRLDLDAPVTTYWPEFGAA
                     GKENIPVSYLLSHQAGLAWTDGENTEEDALNWDVVVALLEAQAPHWEPGTAHGYHATT
                     YGWLVGEVVRRITGQSLGTFFHDDVATPLGLDFWIGLPASEEPRVSTLVSWRDTTALD
                     EATVGAINQFMGPESMIGKALFSPGGALKREDLWNSRELHAAEVPAANGIGDARSIAR
                     MYAACIGDVDGMRLLTPEQLKLATTQQTSGPNTVLMGLDVQFGLGFMLPSSLITIGGP
                     QSFGHFGYGGSMGWADPDAELALGYAMNKLDLVMAGDVRSSSLLDATYAALG"
     gene            complement(2808..3482)
                     /locus_tag="VFX21_02875"
     CDS             complement(2808..3482)
                     /locus_tag="VFX21_02875"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5094925.1"
                     /translation="MDPLGNPVWHALAGPQRTVAEGSARALRFAPDIAPFAALPDDVE
                     PADWEALRVLAGPGGVAVLGRPHVGAPVDLPRVFDIPGVQMVADRSVPDVARPTFRVL
                     GDADADAMAELVARTQPGPWRTRTYTLGTYIGVHDGDRLVAMAGERMRPPGYTEISAV
                     CTDPEYRGRGLAAGLVAELMDRIRARGEIPMLHAAAGNKTAVRLYEMLGFELRRAVEF
                     IGVQFP"
     gene            3481..4380
                     /locus_tag="VFX21_02880"
     CDS             3481..4380
                     /locus_tag="VFX21_02880"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX5094926.1"
                     /translation="MPDCGMARPEERMTFSVLDTASIDGHLARIDDVGYTIVEDAIEP
                     ELVDALRDATRRLERALDLRPRDTDAEGHATLRMYNLLVRDPVFQAMPVHPNILPIVE
                     RLLDRGCLLSGMTAIDIGPGEHPQPLHGDDIVMSRHLQRPHAPMFVTSMWALTDFTAE
                     NGGTVYVPGSHRFAGTPDAPDALRDADIRYLEMRAGSVMVFHGSLWHGGGANTTADEW
                     RLGVNVQYCPGFVRQQQNPYIGIPADVAATFDDRLLSLLGYRLYKGIMGHIDGRSPGE
                     VVFGERFAETAYRDAEQRRARQK"
     gene            4377..>4938
                     /locus_tag="VFX21_02885"
     CDS             4377..>4938
                     /locus_tag="VFX21_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5094927.1"
                     /translation="MTDGTDLTPRIEGSFELADGRQIGYAEYGAPNGRGVFWFHGTPG
                     ARRQMPPAIRSLAEQRNVRLVTLERPGVGTSTRFVYRNVREWSHDVEEIADGLGFERF
                     GLIALSGGGPYLLGCAYELPERVAAGVVLGGVAPSRGPEAVPGGVIGLLTHIAPVLDL
                     YKLPLGITAWGLIRALKPIKSPAFDLY"
BASE COUNT          782 a         1739 c         1723 g          694 t
ORIGIN      
        1 cggcacgagc accgccatca cgacggccgt gtagaccggg acgcggtcgg tgcggcgggc
       61 cgcgaggcgg gcgcgttcga gcggcgggag cggcggccgg ccaccgaatc gacgggcggt
      121 gagcaccgcc gcgtacacga gcaccaagaa cgcgacgaac gcggccagca acgccgccac
      181 ccgcgccgcc cacgagacgc cgagcggaag tcgcagctcg cgcgtcccga gatcgccgag
      241 cagcacgccg acaccgatca cgagccctgc gccgaatgcc gcgcccaggg agagcgcgac
      301 gaacgacacc gcggcggccg gccagcgcga gaaggcgagc agcgccagga tcgcgacgag
      361 ctgcgcgacg aacaagtacg tcacggtgcg atcggtgccc ggcagctgct cgggaagcgg
      421 cgagtgcatc gtcggttcgg tccacgcgaa gaggagcacg cgcccgagcg tcagccacga
      481 gagcgcgagc acgacccagg atgcgatctg ccggcgatgg tcgacgtgcg cgggaacctc
      541 ggggatcgcg agcgccacga tgacgaccaa caacacgacc gcgacaacga gctcgaagcc
      601 catgctgccg gtcgtctccc gcagcgcgta cgacaggagc gcggccagcg ttgcggtcgc
      661 gcccgagatg tggatgtgcc gcaggaagat cccgcgggcg cgcccgtccc acagctgcgg
      721 atggtcgaac ggggtctgca cgacgcgcgg catgaccgtc ggttcgggca cgtacgcctc
      781 cgcccgttgc caggtggccc ggcccagcgc ccacaacaag cacacgaccg cgatcggcaa
      841 cagcaacgcg acgacgaggc ggcgtccggg ggagtcgagc cactcccagc cgaggaagcg
      901 ggtgtagctg tgccgctcga tgcacgcggt gtcgctgccg cactgccacg cgaacagatc
      961 cgcgctcagc acgtacgtcg acaggacgag ggtcgcggtg agcgataggg cgagcagtcg
     1021 ggtcagcggg cgagcgactc tggcgcgagt ccgatgcatc gacgccgccg cgttgaccat
     1081 tgcgaacggt gccagcaaca cccacagcgc gcgccacggg ctgcccgacg tcagcccacc
     1141 ccacgcgtac gcctcgtgat cggtacggtc gtcgggctgg cggccggcac gcggccggta
     1201 gaaccccgac agcgcatcgc ccgtgatcaa ccgcggatgc ggatcgtcga gcagctggtc
     1261 gggcgacgtg cccgacacac cgtgcacccg cacctcgacg atgcccatcg ccgaaatgct
     1321 tccacgcggg cggccgcgcg gcggttaccc ggcgagacgg aggtgggcgc gaggtctcgt
     1381 gcggccctcg agcctctcga gcacgaggcg gacgccgtcg tcgtccggga atgcggcgag
     1441 gtggtcgcgt gcgagtgccg tggctcggtg acggggaccg gcgacgacga cgtccatgat
     1501 gcgccgatac tgctcgtccc tcacgcgctc gaggttcatg gtgtcggtca tttgtcctgc
     1561 ctcggtccga cttctcgtgt ttttcgcact ggcgttagcg tccggatcaa tccgcgctgg
     1621 tggtaggacg acgacgggag cgaaaagtga tcgcgaacgg ttgggtgaag ccgggattcg
     1681 agggcgtccg ggacgcgttc gccgccaatc tcgccgaggg ccgcgacatc ggcgccgcct
     1741 tcagcgccta ccaccggggt gagaaggtcg tcgacctctg gggcggcatc gccgatccgg
     1801 ccaccgaacg tccctgggcc gaggacacgc tcaccctcgt cttctccacg acgaagggcg
     1861 cgaccgcgat ctgcgcccac cgcctcgcgc aggacggccg cctcgatctc gacgcgccgg
     1921 tcacgacgta ctggcccgag ttcggcgccg cgggcaagga gaacatcccc gtctcctact
     1981 tgttgtcgca ccaggccggc ctggcgtgga ccgacggcga gaacaccgag gaggacgcgc
     2041 tgaactggga cgtcgtcgtg gcgctgctcg aagcgcaggc gccgcactgg gagcccggca
     2101 ccgcgcacgg ctaccacgcg acgacctacg gctggctcgt cggtgaggtc gtgcggcgca
     2161 tcacgggcca gagcctcggg acgttcttcc acgacgatgt cgcgacaccg ctcggtctcg
     2221 acttctggat cgggttgccg gcgtccgaag aaccgcgcgt gtcgacgctc gtctcgtggc
     2281 gcgacacgac ggcactcgac gaagcgacgg tcggcgcaat caatcagttc atgggacccg
     2341 agtcgatgat cggcaaggca ttgttctcgc ccggcggtgc gctgaagcgc gaagacctgt
     2401 ggaactcgcg ggagttgcac gcggccgagg tcccggccgc gaacggcatc ggcgacgcgc
     2461 gttcgatcgc gcgcatgtac gcggcatgca tcggcgacgt cgatggcatg cgcttgctga
     2521 cgcccgaaca gctcaagctc gcgacgaccc agcagacgtc gggacccaac accgtgctga
     2581 tgggtctcga cgtgcagttc gggctcggct tcatgctgcc gtcgagcctc atcacgatcg
     2641 gcggcccgca gtcgttcggc cacttcggtt acggcggttc gatgggctgg gccgatcccg
     2701 atgccgagct cgcgctcggc tacgcgatga acaagctcga cctcgtgatg gccggcgacg
     2761 tccgcagctc gtccttgctc gacgccacct acgccgcgct cgggtagcta cgggaactgg
     2821 acgccgatga attcgactgc gcggcgcagc tcgaagccga gcatctcgta gagccgtacc
     2881 gcagtcttgt tgccggccgc cgcgtggagc atcggtatct cgccgcgcgc gcggatgcga
     2941 tccatgagct cggcgacgag tccggccgcg aggccccgcc cgcggtactc cgggtcggtg
     3001 cacaccgcgc tgatctcggt gtagccgggc ggccgcatgc gttcgcccgc catcgccacc
     3061 aggcggtctc cgtcgtgcac gccgatgtag gtgccgagtg tgtacgtgcg cgtccgccac
     3121 ggccccggtt gggtcctcgc gacgagctcg gccatggcgt ccgcgtcggc gtcgccgagc
     3181 acccggaacg tcgggcgcgc gacgtcgggc acgcttcggt cggcgaccat ctggacgccc
     3241 gggatgtcga agacacgcgg gaggtcgacg ggcgccccga cgtgcggacg gccgagcacc
     3301 gccacgccgc ccggccccgc gagcacgcgc agcgcctccc agtcggcggg ttccacatcg
     3361 tccggcagcg ccgcgaaggg cgcgatgtcg ggcgcgaacc tcagggcgcg agcactgccc
     3421 tcggctaccg ttcgttgcgg tccggcgagc gcgtgccaca cggggttgcc gaggggatcc
     3481 atgcccgatt gtggcatggc ccggccggag gaacggatga ctttttcggt gctcgatacc
     3541 gcgtcgatcg acgggcatct cgcgcgcatc gacgatgtcg gctacacgat cgtcgaggac
     3601 gcgatcgagc ccgagctcgt ggacgcgctg cgcgacgcga cccgccgcct cgaacgcgcg
     3661 ctcgacctcc ggccccgcga caccgatgcc gaaggccatg cgacgctgcg catgtacaac
     3721 ctgctcgtac gcgacccggt gttccaggcc atgccggtgc acccgaacat cctcccgatc
     3781 gtcgagcgac tgctcgaccg cgggtgcctc ctgtcgggaa tgacggcgat cgacatcggt
     3841 cccggcgaac atccgcaacc cctgcacggc gacgacatcg tcatgtcacg ccatctgcag
     3901 cggccgcacg cgccgatgtt cgtcacgagc atgtgggcgc tcaccgactt caccgccgag
     3961 aacggcggca ccgtgtacgt gcccggttcg catcgtttcg ccggcacgcc cgacgcgccc
     4021 gacgcgttgc gcgacgccga catccggtac ctcgaaatgc gcgcgggatc cgtgatggtg
     4081 ttccacggca gcctgtggca cggcggcggc gcgaacacga ccgccgacga atggcggctc
     4141 ggcgtcaacg tgcagtactg cccggggttc gtgcgccagc agcagaatcc gtacatcggc
     4201 atccccgcgg acgtcgcggc caccttcgac gaccggttgc tgtcactgct cgggtaccgg
     4261 ctgtacaagg gcatcatggg acacatcgac ggccgcagcc cgggtgaagt cgtgttcggc
     4321 gaacgcttcg ccgagaccgc ttaccgcgac gccgagcaac gccgcgcccg gcagaagtga
     4381 ccgacggtac cgatctcacg ccgcgcatcg aaggttcctt cgagctcgcc gacggccgcc
     4441 agatcggata cgccgaatac ggcgcgccca acgggcgcgg tgtgttctgg ttccacggca
     4501 cacccggtgc gcgccgccag atgccgcccg cgattcgctc gctcgcggag caacgcaacg
     4561 tgcggctcgt cacgctcgaa cgacccggcg tcgggacgtc gacgcgtttc gtgtaccgca
     4621 atgtgcgcga gtggtcccat gacgtcgagg agatcgccga cggcttgggc ttcgagcgct
     4681 tcgggctcat cgcgctctcg ggcggcgggc cgtacttgct gggttgcgcg tacgagctgc
     4741 ccgagcgcgt cgccgcgggc gtcgtgctcg gtggcgtcgc gccgtcgcgc gggccggaag
     4801 ccgtcccggg cggtgtcatc ggcttgctga cacacatcgc gccggtgctc gacctctaca
     4861 agctcccact ggggatcacc gcgtgggggc tgatccgcgc gctcaaaccg atcaagtcgc
     4921 cggctttcga cctctacg
//
LOCUS       DASUGA010000150         2174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1987669, whole genome shotgun sequence.
ACCESSION   DASUGA010000150 DASUGA010000000
VERSION     DASUGA010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1987669"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..560
                     /locus_tag="VFX21_02890"
     CDS             117..560
                     /locus_tag="VFX21_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX5094928.1"
                     /translation="MALAGEYEPSPWDWVRDQVETYERTGGREANTFMDTGLPIIILT
                     TRGNKTGKIRKTPLMRVEHEGEYALVASVGGAPKHPVWYFNVVADPDAATVQDGPEPF
                     AVRLHEATGAERDIWWKRAVAAYPPYAEYQERTERVIPVFIARRV"
     gene            560..1312
                     /locus_tag="VFX21_02895"
     CDS             560..1312
                     /locus_tag="VFX21_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEX5094929.1"
                     /translation="METVLAALTEQHTELDALLSPLDHKGWARPTRCEGWDVADVVLH
                     LAQTDELAIASAELRVNEQLGSLGGPEHDNTVDEGAADMVALERGRPHAALLLRWRDG
                     ASLLRAVLGASDPHQRVQWVAGKLSIHTLCATRLSECWIHTGDVAGALGVEQEVTDRL
                     EHVARLAWRTLPYAFARAGRELQGPVEFDLRAPSGARWHFVPEGEPVTRVEGDGAELC
                     LVAARRVTPDETSLRATGPDADAVLECVRTYA"
     gene            complement(1314..>2174)
                     /locus_tag="VFX21_02900"
     CDS             complement(1314..>2174)
                     /locus_tag="VFX21_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HEX5094930.1"
                     /translation="GWTDISQRIRDRILLEVGKGNEFTPELVERAFADSDDEKMEEIR
                     ARCNAIVEDEITAENLKAWYRQLCKRPCFHDEYLQAYNEPTCHLVDTDGKGVTRIDET
                     GAWVGDDHYELDCLIFASGFEVGTSYDRRSGYDTIGPGGNRLSRAWEDGMRSMHGIHV
                     HGFPNLFIIGFAQGANLISNITSNLADAGVTIATVVRHALDTGAAEVEVTADAEDAWM
                     ELLESSPMSTVFGSPDCTPGYYNNEGQPLNSRARFAMAGYPYGPVAYFDYIDKWRTAG
                     DFEGLEFRSR"
BASE COUNT          353 a          732 c          742 g          347 t
ORIGIN      
        1 gtgcggtccc cacagctcgt tgaacgcgcc gtgcaccaag acgatacgag ccacccgttg
       61 ctccttccga ggaacgcccg cagcttcgca cagcggtgcc gcagtacggt gctgccatgg
      121 cgctcgcagg cgagtacgaa cccagcccgt gggactgggt gcgcgaccag gtcgagacgt
      181 acgaacgcac cggcggcaga gaagccaaca cgttcatgga caccggcctg ccgatcatca
      241 tcctcacgac acgcggcaac aagacgggca agatccgcaa gacacccttg atgcgcgtcg
      301 agcacgaggg cgagtacgcg ttggtcgcgt cggtcggtgg cgcgccaaag cacccggtct
      361 ggtacttcaa cgtcgtggcc gaccccgacg cggcgaccgt tcaagacggc cccgagccat
      421 tcgcggtgcg gctgcacgaa gcaacgggtg ccgagcgcga catctggtgg aagcgggcgg
      481 tcgccgccta cccgccctac gccgaatatc aggaacgcac cgaacgtgtc atcccagtgt
      541 tcatcgcgcg gagggtgtga tggaaaccgt gctggctgcg ctgaccgaac agcacaccga
      601 gctcgacgcg ttgctgtcac cgctcgacca caagggatgg gctcggccga cgcgctgcga
      661 ggggtgggac gtcgccgacg tcgtcttgca cctcgcccag accgacgagc tcgcgatcgc
      721 gagcgccgag ctgcgggtca acgagcaact gggatctctc ggcgggccgg agcacgacaa
      781 cacggtcgac gagggtgcag ccgacatggt cgcgctcgaa cgcggccggc cccacgcggc
      841 gctgctcctg cgctggcgcg acggcgcgtc cttgttgcgt gcggtgctcg gcgccagcga
      901 tccacatcaa cgggtgcaat gggtcgcggg gaagctgtcg atccacacgt tgtgcgcgac
      961 ccgactgtcg gagtgctgga tccacacggg cgacgtcgcc ggcgcgctgg gcgtcgagca
     1021 agaggtcacc gaccgcctcg aacacgtcgc ccgcctcgcg tggcgcacgc ttccctacgc
     1081 gttcgcgcgc gccggacgcg aactgcaggg accggtcgag ttcgacttgc gggccccgag
     1141 cggtgcgcgg tggcacttcg tgcccgaggg cgagccggtg acgcgcgtcg agggcgacgg
     1201 tgccgagctg tgcctggtcg cggcgcgccg ggtcaccccc gacgagacgt cgctgcgcgc
     1261 caccggcccc gacgccgacg cggtgctgga gtgcgttcgc acctacgcgt gactcaacgc
     1321 gagcggaact cgaggccttc gaagtcgccc gccgtgcgcc acttgtcgat gtagtcgaaa
     1381 tacgcaaccg ggccgtacgg atacccggcc atcgcgaagc gggcacggga gttcagcggt
     1441 tggccttcgt tgttgtagta gccgggcgtg cagtcggggc tgccgaagac cgtcgacatc
     1501 gggctgctct ccagcaactc catccacgcg tcctcggcat cggctgtgac ctcgacctcg
     1561 gcggcgccgg tgtcgagcgc gtggcgcacc accgtcgcga tcgtcacgcc cgcgtcggcg
     1621 aggttcgacg tgatgttcga gatgaggttt gcgccctgcg cgaacccgat gatgaacagg
     1681 ttcgggaagc cgtgcacgtg gatgccgtgc attgagcgca tgccgtcttc ccacgcgcgc
     1741 gagaggcgat tgccaccggg accgatcgtg tcgtagcccg agcggcggtc gtagctggtg
     1801 ccgacttcga agcccgacgc gaagatcagg cagtcgagct cgtagtggtc gtcgccgacc
     1861 caggcgccgg tctcgtcgat ccgtgtcaca cccttgccgt cggtgtcgac gaggtgacag
     1921 gtcggctcgt tgtaggcctg cagatactcg tcgtggaagc acggacgctt gcacagctgc
     1981 cggtaccacg ccttcaggtt ctcggcggtt atctcgtctt cgacgatcgc gttgcaacgc
     2041 gcgcggatct cttccatctt ctcgtcgtcg ctgtcggcga acgcgcgctc gaccaattca
     2101 ggggtgaact cgttgccctt gcctacctcc aacaagatgc ggtcgcggat ccgctgcgag
     2161 atgtcggtcc agcc
//
LOCUS       DASUGA010000151         2109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_649554, whole genome shotgun sequence.
ACCESSION   DASUGA010000151 DASUGA010000000
VERSION     DASUGA010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649554"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(348..473)
                     /locus_tag="VFX21_02905"
                     /pseudo
     CDS             complement(348..473)
                     /locus_tag="VFX21_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019069961.1"
                     /note="Catalyzes the reversible hydration of unsaturated
                     fatty acyl-CoA to beta-hydroxyacyl-CoA; incomplete;
                     partial in the middle of a contig; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
     gene            526..1386
                     /gene="purU"
                     /locus_tag="VFX21_02910"
     CDS             526..1386
                     /gene="purU"
                     /locus_tag="VFX21_02910"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013523764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEX5094931.1"
                     /translation="METGTERGVLLLSCPDQPGIVAAVATFIADQGGNIVHAEQHTDF
                     DDGVFFQRVEFELDGLAERADELQQAFQPVAARFGMEVTARLSGETARVAVLVSREAH
                     CLQDLLYRWRSGELQADIPVVISNHPDHAELVEFQGSKYVHLPVTPETKPEQERAIRS
                     VLEEHRIDLVVLARYMQILSREFVEDHRNRIINIHHSFLPAFAGARPYHQASKRGVKI
                     IGATAHYVTEELDAGPIIEQDVARVTHRDHADAMVRLGRDIERTVLARAVKWHLEDRV
                     LVHGNRTVVF"
     gene            complement(1383..>2109)
                     /locus_tag="VFX21_02915"
     CDS             complement(1383..>2109)
                     /locus_tag="VFX21_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX5094932.1"
                     /translation="PENVAPAGETTVQFVRTIPERAYRALPRGEFSVLESYVRALRSA
                     RRLIYLENQFLWSPEIVDILKAKLRAPPSDDFRVVVLLPHRANNGQDDTRGMLARLVD
                     ADAHDRFLAATIMSRSGERSGPLYVHAKIGIVDDAWLAIGSANLNEHSLFNDTEVDVV
                     THDPALARDTRLRLWAEHLERDDVGGDPARLVDEQWIPTAQEQLARSRRGEPLTHHLV
                     ELPGVSRRSRRLLGPLDALVVDG"
BASE COUNT          322 a          716 c          755 g          316 t
ORIGIN      
        1 agcacaccga cttcgacgac ggtgtcttct tccagcgcgt ggagttcgag ctcgacggct
       61 tggcagagcg tgccgacgag ctgcagcagg cgttccaacc cgtggctgca cgcttcggca
      121 tggaggtcac cgcgaggttc agcggggaga cgccgcgggt cgcggtgctc gtgtcacgcg
      181 aggcgcactg cctccaggac ctcctctacc ggtggaagag tggtgagctc gaggccgacg
      241 tcccggtcgt gatcagcaac catcccgacc acaccgagct cgtcgagttc cagggctcga
      301 agtacgtgca cctcccggtc acgcccgaga cgaagcccga ccaggagcgc gcggtcgagc
      361 tcgtcgagca gctggctgtt ctgcgcgttc gccgcagcgg gacggttcag cgtgatcgtg
      421 gcgaccgcgc cgtccgtctc gtaacggatc gtctcgaacc cggcacctga catcgatgtc
      481 acgttaggcg tcgcggcgtg gggttcgctg cctaccattc caaccgtgga gacgggcacg
      541 gaacgcggcg tcctgctctt gtcctgtccc gatcagccgg ggatcgtcgc cgcggtcgcg
      601 accttcatcg ccgaccaggg cggcaacatc gtgcacgccg agcagcacac cgacttcgac
      661 gacggtgtct tcttccagcg cgtggagttc gagctcgacg gcttggcaga gcgtgccgac
      721 gagctgcagc aggcgttcca acccgtggct gcacgcttcg gcatggaggt caccgccagg
      781 ctcagtgggg agacggcgcg ggtcgcggtg ctggtgtcgc gggaggcgca ttgcctccag
      841 gacctcctgt accggtggag gagcggcgag ctccaggccg acatcccggt cgtgatcagc
      901 aaccatcctg atcacgcgga gctcgtggag ttccagggct cgaagtacgt gcacctcccg
      961 gtcacgcccg agacgaagcc ggagcaggag cgcgcgatcc gcagcgtgct cgaggagcac
     1021 cggatcgatc tcgtcgtgct cgcccgctac atgcagatcc tcagccgcga gttcgtcgag
     1081 gatcaccgca accggatcat caacatccac cactcgttcc tcccggcatt cgccggcgcg
     1141 cggccgtacc accaggcgag caagcgcggc gtcaagatca tcggcgcgac cgcgcactat
     1201 gtgaccgagg agctcgacgc cggcccgatc atcgagcagg acgtcgcgcg cgtgacccac
     1261 cgcgaccacg ccgacgcgat ggtgcggctc ggccgcgaca tcgagcgcac ggtgctggcg
     1321 cgcgcggtca agtggcacct cgaggaccgc gtgctcgtgc acggcaaccg caccgtggtc
     1381 ttctaaccgt cgaccacgag cgcgtcgagc gggccgagca gccggcgcga gcggcgcgac
     1441 acgcccggca gctcgaccag gtggtgcgtg agcggctcgc cgcggcggct gcgcgcgagc
     1501 tgctcctgcg ccgtcgggat ccactgctcg tcgaccagcc gcgcggggtc gccgccgacg
     1561 tcgtcgcgct cgaggtgctc ggcccacagc cgcagccgcg tgtcgcgggc gagcgccggg
     1621 tcgtgggtga cgacgtcgac ctcggtgtcg ttgaacagcg agtgctcgtt gaggttggcc
     1681 gagccgatcg ccagccacgc gtcgtcgacg atgccgatct tggcgtgcac gtagagcggg
     1741 ccggagcgct cgccggagcg cgacatgatc gtggccgcca ggaaccggtc gtgcgcgtcg
     1801 gcgtcgacca gccgcgccag catcccgcgc gtgtcgtcct ggccgttgtt ggcgcggtgc
     1861 gggagcagga cgacgacgcg gaagtcgtcg gacggcggcg cgcgcagctt cgccttgagg
     1921 atgtcgacga tctccggcga ccacaggaac tggttctcga ggtagatcag gcggcgggcg
     1981 ctgcgcagcg cgcgcacgta ggactcgagc accgagaact cgccgcgcgg tagggcgcgg
     2041 taggcgcgct cggggatcgt gcggacgaac tggacggtgg tctcgccggc gggcgcgacg
     2101 ttctcgggc
//
LOCUS       DASUGA010000152         4587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1308158, whole genome shotgun sequence.
ACCESSION   DASUGA010000152 DASUGA010000000
VERSION     DASUGA010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308158"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VFX21_02920"
     CDS             complement(<1..205)
                     /locus_tag="VFX21_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5094933.1"
                     /translation="MGNEPAIGHWRTPAAERDFLALFDELRAEQFGGFDDRDVETRYG
                     TTRVYHRRGPGVPIVMLPGAGAPA"
     gene            complement(296..1255)
                     /gene="pip"
                     /locus_tag="VFX21_02925"
     CDS             complement(296..1255)
                     /gene="pip"
                     /locus_tag="VFX21_02925"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003991669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEX5094934.1"
                     /translation="MSGGDDEAYETGDLPVGDGHVLHWEQSGNRDGKPVVVLHGGPGS
                     GSSPAFRLMFDPGVYRVVLFDQRNCGQSTPHASDAGVSLARNTTPALVADIERLRAHL
                     GIERWMVWGGSWGTTLGLAYAQVHPDRVTEIILTNVGLTSRREVEWVTRAMGRIFPEE
                     WSRFVAVVPESERDGDLAAAYARLLADPDSEVRERAAAAWCEWEDTHVGTYVRHQHDP
                     RYDDPRFRMCFARIVTHYWANAAFLPDGQLLRDAHLLDGIPGVMIQGHLDISSPPDVA
                     WQLAQRWTTSELVLVDDAGHGAGHPSTYEAIIAATQRFGRPRT"
     gene            complement(1266..1910)
                     /locus_tag="VFX21_02930"
     CDS             complement(1266..1910)
                     /locus_tag="VFX21_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5094935.1"
                     /translation="MSAGPSDATARALPGRPRRYGPDEELRLLFDAAIEVMRRNEYGE
                     VTVAEILTEAGVSTRSFYRHFASKDDLLIALYRRETDGAVAALEARVARAKHPRDALE
                     VWVDEMLSLTHDRAKARKVDVLVSPAALRANGSTAEVQRATEVLRRPLVMILTAGRID
                     GSFKTVEPFDDADAISKLVWDVARQGFAGTDTRSLKEARAFVLSFSLRAIGGTL"
     gene            complement(1907..2665)
                     /locus_tag="VFX21_02935"
     CDS             complement(1907..2665)
                     /locus_tag="VFX21_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5094936.1"
                     /translation="MEIAGKKAVVVGGASGMAKATAEMLAAKGAKVAILDLPTSAGAE
                     VAKALDGYFAPVDIMDDEGTEQALAAVVEQLGGLHIAVNTAGGGTAMRTLSKNGPHPL
                     AEFRRVVELNLIGSFNLNRLQADYMSKNEPEDDERGVIIDTASIAAFEGQIGQVAYTA
                     AKAGIAGMVFTMARDLGSLGIRVLAIAPSLFSTGITAGIPDEFAAVLTKDAAFPKRMG
                     RPEEYGKLALAIVENPMLNGLTIRLDAGTRFAPK"
     gene            2729..>4587
                     /locus_tag="VFX21_02940"
     CDS             2729..>4587
                     /locus_tag="VFX21_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX5094937.1"
                     /translation="MSIAITEDHRALADTVADFLTKREARKAARALLEAPAEGLPDFW
                     DDLRGLGWLGLHVPEAQGGSGYGLPELLIVVEEMGRAIAPGPFVPSVIASATLAAAAP
                     DALAAKYLPDLASGATVGAVALDGDVAVSGGTATGSAGVVLGGGLADILLVGVGDDVA
                     IVDARNGNVSIEVPPNLDPTRRAARITLDGAAAELIPGARTTMLDFARAIIAAEATGI
                     ARETTVLAAEYAKVREQFGRPIAMYQAVKHHCANMLVAAELATALVWDAARASTHADE
                     QFSLTAAMAAALAVPAADWNSQLNMQVHGGIGFTWEHDAHLYLRRGTAIGAIINAEDA
                     REHVTELTRRGVTRSKSVDLPPEAEPIRDTVRAFVARVKDLDADATKAALIESGYAMP
                     HWPAPWGREAGAIEQLVIEQEFAAAGVKRPAYGITGWVILTLIQHATEDQVSRWVPAA
                     LRQDLIWCQLFSEPDAGSDAAGIKTRATRTDGGWLVNGQKVWTSGAHVAKYGFATVRT
                     NPDVAKHDGITTMVIDMEAPGVEVRPLKMPTGNSEFNEVFFNDVFVPDDDVVGPVDGG
                     WTVARATLGNESVSIGGGQGGGGMPVDGFIAPLDAHPDRLPGGAGRVGRYIATS"
BASE COUNT          708 a         1617 c         1563 g          699 t
ORIGIN      
        1 gcgccggcgc gccggcgccc ggcagcatca cgatcggaac acccgggccg cggcggtggt
       61 acacgcgggt cgtgccgtag cgggtctcga cgtcgcgatc gtcgaagccg ccgaactgct
      121 ccgcgcgcag ttcgtcgaac agggcgagga agtcgcgttc ggcggcgggc gtcctccagt
      181 gaccaatggc aggttcgttg cccacgatga ccacctctcg ttgtggtccg agcgtaggtg
      241 agatcctgcg ccaccactga atgcgcgcct acgcaacatc atcggcgaac gaaaatcacg
      301 tacgcggtcg accgaatctt tgggtcgcgg caatgatcgc ctcgtaggtc gacgggtgcc
      361 cggcgccgtg cccggcgtcg tcgacgagga ccagctccga agtcgtccat cgttgcgcga
      421 gctgccacgc gacgtcgggc gggctgctga tgtcgaggtg cccctggatc atgacgcccg
      481 ggatcccgtc gagcaggtgc gcgtcgcgca ggagttgacc atcgggcagg aaggcggcgt
      541 tcgcccagta gtgcgtgacg atgcgggcga agcacatgcg aaagcgcggg tcgtcgtagc
      601 gcgggtcgtg ttggtgcctc acgtaggtgc cgacgtgcgt gtcctcccac tcgcaccacg
      661 ccgcggccgc ccgttcgcgc acctccgaat cggggtcggc caatagtcgc gcataggccg
      721 cggccagatc gccgtcgcgc tcggactcgg gaacgacggc gacgaaacgc gaccactcct
      781 ccgggaagat gcggcccatc gcgcgcgtca cccactcgac ttcgcgacgc gacgtgagac
      841 cgacgttggt gaggatgatc tcggtgactc ggtcggggtg cacctgcgcg tacgcgagac
      901 cgagcgtggt gccccacgaa ccaccccaca ccatccaccg ttcgatgccg aggtgggcgc
      961 gcaatcgttc gatgtcggcc accagcgcgg gcgtcgtgtt ccttgcgagc gacacaccgg
     1021 cgtcgctcgc gtgcggcgtg ctctgcccgc aattgcgctg gtcgaacaac acgacgcggt
     1081 acacgccggg gtcgaacatg aggcggaagg cgggcgacga accggacccg gggccgccgt
     1141 gcagcacgac caccggcttg ccgtcgcggt tcccgctctg ctcccagtgc aggacgtgac
     1201 cgtcaccgac cggcagatcg ccggtctcgt acgcctcgtc gtccccgccg ctcatcgggg
     1261 agaagttaga gcgtaccgcc gatcgcgcgc agcgagaacg acagcacgaa cgcgcgcgct
     1321 tctttgaggc tgcgcgtgtc ggtcccggcg aaaccttggc gggcgacgtc ccacaccagc
     1381 ttgctgatcg cgtccgcgtc gtcgaacggt tcgacggtct tgaacgaccc gtcgatccgg
     1441 cccgcggtca ggatcatcac gagtggccgc cgcaggactt ccgtcgcgcg ctgcacttcg
     1501 gccgtcgaac cattggcgcg cagcgccgcc ggcgacacca acacgtcgac cttgcgggcc
     1561 ttggcgcgat cgtgcgtgag gctcagcatc tcgtcgaccc agacctcgag cgcatcacgt
     1621 gggtgcttcg cgcgcgcgac ccgtgcttcc aacgccgcca ccgcgccatc cgtctccctg
     1681 cggtagagcg cgatcagcaa gtcgtccttc gacgcgaagt gccggtagaa cgagcgcgtc
     1741 gacacgcctg cttccgtgag aatctccgcg accgtgacct cgccgtactc gttgcggcgc
     1801 atgacttcga tggccgcgtc gaacaacagc cgcagctctt cgtccggtcc gtaccgtcgc
     1861 ggccgccccg gcagcgcccg cgcagttgcg tcgctcggcc ccgcgctcac ttcggcgcga
     1921 aacgcgttcc cgcgtcgagg cggatcgtga gcccgttgag catcgggttc tcgacgatcg
     1981 cgagcgcgag cttcccgtac tcctccggcc gtcccatccg cttcgggaac gcggcgtcct
     2041 tggtgagcac ggcggcgaac tcgtcgggga tgccggcggt gatgccggtg ctgaacaagc
     2101 tcggcgcgat cgcgagcacg cggatcccga ggctgcccag gtcgcgcgcc atcgtgaaca
     2161 ccatgcccgc gatgccggcc ttggctgcgg tgtacgcgac ctgaccgatc tggccctcga
     2221 aggccgcgat cgatgcggtg tcgatgatca cgccgcgctc gtcgtcttcg ggctcgttct
     2281 tcgacatgta gtcggcctgg aggcggttca ggttgaagct gccgatcagg ttcagctcga
     2341 cgacacgccg gaactcggcc agtgggtggg gtccgttctt cgacaacgtg cgcatcgcgg
     2401 ttccgccgcc cgcagtgttg accgcgatgt gcagtccgcc gagctgttcg acgactgcgg
     2461 ccaatgcctg ctcggtgcct tcgtcgtcca tgatgtcgac aggagcgaaa tagccgtcga
     2521 gcgccttcgc cacctcggcg ccggccgacg tcggcaggtc gaggatcgcg accttggcgc
     2581 ccttggccgc gagcatctcg gccgtcgcct tcgccatccc cgacgcgccg cccacgacga
     2641 cggccttctt ccctgcgatc tccactgcgg ttgctcccct cttggaccgg ccgggcgaga
     2701 gagtaaccgc cttgcgtatg gttcccgcat gtcgatcgcc atcaccgaag accaccgggc
     2761 tctcgccgac acggtggcgg acttcctgac caagcgcgaa gcccgcaagg cggcccgcgc
     2821 gttgctcgaa gcaccggcgg aagggctgcc ggacttctgg gacgacctgc gcgggctcgg
     2881 atggctcggg ctgcacgtgc ccgaagcgca gggcggctcc ggctacggcc tgcccgagtt
     2941 gctcatcgtg gtcgaagaga tgggacgcgc gatcgcgccg ggcccctttg tcccgtcggt
     3001 gatcgcgagc gcgacgctcg ccgcggccgc gcccgacgca ctcgccgcga aatacctccc
     3061 cgacctcgcg agcggcgcga ccgtcggcgc ggtcgcgctc gacggtgacg tcgccgtcag
     3121 cggcggcacc gcgaccggtt ccgccggcgt cgtgctcggc ggcggactcg ccgacatcct
     3181 cctcgtcggc gtaggtgacg acgtcgcgat cgtcgacgca cgcaacggca acgtgagcat
     3241 cgaagtcccg cccaacctcg acccgacacg acgcgccgcg cgtatcacgt tggacggcgc
     3301 cgcggccgaa ctcattcccg gcgcgcgaac gacgatgctc gacttcgcgc gcgccatcat
     3361 tgccgccgag gcaaccggca tcgcgcgtga gacgactgtg ttggccgcgg agtacgcaaa
     3421 ggtgcgcgag cagttcgggc gcccgatcgc gatgtaccag gcggtcaagc accattgcgc
     3481 caacatgctc gtcgcggccg agctggcgac cgcgctcgtc tgggacgcgg cgcgcgcgtc
     3541 gacacatgcc gacgaacagt tctcgttgac cgccgcgatg gcggccgcgc tcgcggttcc
     3601 cgcggccgac tggaactcgc agctgaacat gcaggtgcac ggcggcatcg gcttcacgtg
     3661 ggagcacgac gcgcacctgt acctgcgccg cggcaccgcg atcggagcga tcatcaacgc
     3721 ggaggacgcg cgcgagcacg tcaccgagct gacccgccgg ggcgtgaccc gttcgaagtc
     3781 ggtcgacctg ccgcccgaag ccgaaccgat ccgcgacacc gtgcgtgcgt tcgtcgcgcg
     3841 cgtcaaggac ctcgatgccg acgcgaccaa ggccgcgctg atcgagtcgg gctacgcgat
     3901 gccgcactgg ccggcgccat ggggccgcga agccggcgcg atcgaacaac tcgtgatcga
     3961 gcaggagttc gccgcggccg gcgtgaagcg acccgcatac ggcatcacgg gctgggtgat
     4021 cctgacgctc atccagcacg cgaccgaaga ccaggtgagc cgttgggtgc cggcggcgtt
     4081 gcgccaagac ctgatctggt gccagctctt cagcgagccc gacgcgggct cggacgcggc
     4141 aggcatcaag acgcgcgcca cccgcaccga cggcggctgg ctcgtcaacg gccagaaggt
     4201 gtggacgagc ggcgcgcacg tcgcgaagta cggcttcgcc accgtgcgca cgaaccccga
     4261 cgtcgccaag cacgacggca tcacgaccat ggtcatcgac atggaggcgc cgggcgtcga
     4321 agttcggccg ttgaagatgc cgaccggcaa ctccgagttc aacgaggtgt tcttcaacga
     4381 cgtcttcgtg cccgacgacg acgtcgtcgg tccggtcgac gggggatgga ccgttgcccg
     4441 ggcgacgctc ggcaacgaga gcgtgagcat cggcggtggc cagggcggcg ggggcatgcc
     4501 cgtcgacggg ttcattgcgc ccctcgacgc gcacccggat cgtttgccgg gcggcgcagg
     4561 acgagtcggc cgctacatcg cgacatc
//
LOCUS       DASUGA010000153         3119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1925787, whole genome shotgun sequence.
ACCESSION   DASUGA010000153 DASUGA010000000
VERSION     DASUGA010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1925787"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /gene="hemC"
                     /locus_tag="VFX21_02945"
     CDS             <1..950
                     /gene="hemC"
                     /locus_tag="VFX21_02945"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX5094938.1"
                     /translation="RLRVATRGSRLAKWQAERVASLLGGDVEFVLIETTGDIDKTSDL
                     HAIGGQGVFVKEVQHAVLDGRADLAVHSAKDLPSETPDGLVIASVPERADARDALVGA
                     RLDDIPTGGLVGTGSVRRRAQLAYARPDLMFTGLRGNIETRLRKQRDEGMDAIVVALA
                     ALERLALTGHVTEVLEPGVMLPQVAQGALAIECRADDDATRARTGAIEDRAVRRAVDA
                     ERAYLRTLGGGCDLPIGALAVVDGDEIVLDALLATPDGHSIVRTRVSGTDPDDVGETA
                     AKHLLTQASIAPTPRPHPPPNEETNHGGLSTPVSPGETA"
     gene            947..2422
                     /gene="cobA"
                     /locus_tag="VFX21_02950"
     CDS             947..2422
                     /gene="cobA"
                     /locus_tag="VFX21_02950"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX5094939.1"
                     /translation="MTVYLVGAGPGDPGLLTRRGEELLRRADVVVYDRLASRALLDLA
                     PAHAERIDVGKAPGRAAMTQDEINTVLVEHGRAGREIVRLKGGDPFVFGRGGEEAEAC
                     IAAGVPFEVVPGVTSAIAAAAYAGIPVTHRGVSTSVTIVTGHEDPAKGTIDTDWDALA
                     KHGGTLVVLMGAGHIADIATALMKGGRDASTPVAAVRWGTRPEQRTIRTTLAHVADAG
                     IESPSAIVVGDVAALDFAWFEQRPLFGTRIVVTRAREQASRLRADLEALGAEVIELPS
                     IAIAPAEFTVPELANYAWLVFTSANGVDAFFDRGLAGRDSRALAPVRVAAIGPGTADA
                     LARRGIVADLVPERFVAESLLDAFPAPAANSERLLLARAAHARDVLPDGLAERGYAVE
                     VLAVYETVAAEADPADLERIRAGKVDAVTFTSSSTVTNFCDLVGALPEPPPKIVSIGP
                     VTSATAAERGLTVTTEADPHTIAGLVAAVVRTLGSTASAPG"
     gene            2507..>3119
                     /locus_tag="VFX21_02955"
     CDS             2507..>3119
                     /locus_tag="VFX21_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017585817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX5094940.1"
                     /translation="MSFPERRLRRLRRTPAIRRLVAEHRLTVDDLIAPLFVKEGIDAP
                     APIGSMPGVVQHTQESLRKEVRALADLGIPGVILFGVPAHKDARGSQADADDGVVQVA
                     LRNLRDDVGDEIVLMVDNCLDEYTDHGHCGILTDRGDVDNDATIERYASIAVAQAAAG
                     ADVIAPSGMMDGQVGAIRTGLDDAGFDQTSILAYAAKFASALYG"
BASE COUNT          461 a         1127 c         1099 g          432 t
ORIGIN      
        1 cccgcctgcg cgtcgcgacg cgcgggagcc gcctcgccaa atggcaggcc gagcgcgtcg
       61 cctcgctgtt gggtggcgac gtcgagttcg tgttgatcga gacgaccggc gacatcgaca
      121 agacctccga tctgcacgcg atcggcgggc agggcgtgtt cgtgaaagag gtgcagcacg
      181 ccgtgctcga cgggcgggcc gacctcgcgg tgcactcggc caaggatctc ccctccgaga
      241 caccggacgg cctggtgatc gcatcggtgc cggagcgcgc cgatgcgcgc gacgcgttgg
      301 tgggtgcccg gctcgacgac atcccgaccg gcggtctcgt cggcaccgga tccgtgcgcc
      361 ggcgggccca gcttgcgtac gcgcgtccgg atctcatgtt caccgggttg cgcggcaaca
      421 tcgagactcg cttgcgcaaa caacgcgacg aagggatgga cgcgatcgtc gtcgcgttgg
      481 ccgcgctcga acggttggcg ctcaccgggc acgtcacgga agtgctcgag cccggcgtga
      541 tgctgccgca ggtcgcacaa ggcgcgctcg cgatcgagtg ccgcgccgac gacgacgcga
      601 cgcgcgcccg taccggtgcg atcgaggacc gagccgtacg acgcgcggtc gacgcggaac
      661 gcgcttacct gcgcacactc ggcggcggct gcgacctccc gatcggcgcg ctggcggtcg
      721 tcgacggtga cgagatcgtc ctcgacgcgc tcctcgcgac ccccgacggc cactcgatcg
      781 tccgcacgag ggtgagcggc accgatcccg acgacgtcgg cgagacggcc gcaaaacacc
      841 tgctcacaca ggcgtcgatc gcccccaccc cccgacccca cccaccgccc aatgaggaaa
      901 cgaaccacgg tgggttatcg acgcctgtct cacccgggga aaccgcgtga ccgtctatct
      961 ggtcggggcg gggccgggcg acccggggtt gctcacacga cgcggtgaag agctgctccg
     1021 gcgcgcggac gtcgtcgtgt acgaccggtt ggcgtcgcgc gcgctgctcg acctcgcacc
     1081 tgcgcacgcc gaacgcatcg acgtcgggaa ggcgcccggt cgcgccgcga tgacccagga
     1141 cgagatcaac accgtgctcg tcgagcacgg acgcgccggt cgggaaattg tgcggctcaa
     1201 gggcggtgat ccgttcgtgt tcgggcgcgg cggcgaggag gccgaggcgt gcatcgcggc
     1261 gggtgtgccg ttcgaagtgg tgccgggcgt gacgagcgcg atcgcggcgg ctgcctacgc
     1321 cggcattccg gtgacgcatc gcggcgtctc cacgagcgtc acgatcgtca cgggtcacga
     1381 ggacccggcc aagggcacga tcgacaccga ctgggacgcg ctggcgaagc acggcggcac
     1441 gctcgtcgtc ctcatgggtg cgggacacat cgccgacatc gcgaccgcgt tgatgaaggg
     1501 cgggcgcgac gcatcgacac ccgttgccgc cgtgcgctgg ggcacccgtc ccgagcagcg
     1561 cacgatccgc acgacgctgg cccacgtcgc ggacgccggg atcgaatcgc cctcggcgat
     1621 cgtcgtcggc gacgtcgccg ccctcgactt cgcctggttc gagcagcgac ctctgttcgg
     1681 cacgcgcatc gtcgtgaccc gtgcccgcga gcaggcgagc cgattgcggg ccgatctcga
     1741 agcactcggt gcggaggtga tcgagctgcc gtcgatcgcg atcgcgccgg ccgagttcac
     1801 ggtgcccgag ctcgcgaact acgcgtggtt ggtgttcacg tccgcgaacg gtgtcgacgc
     1861 gttcttcgac cgaggtctgg ccggccgcga ctcccgcgcg ctcgcgccgg tgcgcgtcgc
     1921 cgcgatcggc ccgggcaccg ccgacgcgct cgcgcgccgc ggcatcgtcg ccgatctcgt
     1981 ccccgagcgc ttcgtcgccg agtccttgct cgacgcgttc ccggcgccgg cggccaacag
     2041 cgaacgcctc ctgctcgcgc gcgccgcaca cgcgcgcgac gtgctgcccg acgggttggc
     2101 cgaacgcggc tacgccgtcg aggtgctcgc ggtctacgag accgtcgcgg ccgaagccga
     2161 tccggctgat ctcgaacgca tccgcgcggg caaggtcgac gcggtgacgt tcaccagttc
     2221 gtcgacggtc accaacttct gcgacctcgt cggcgcgctc ccggagccgc ccccgaagat
     2281 cgtctcgatc ggcccggtca cgtccgcgac ggccgccgag cgaggcttga ccgtgacgac
     2341 ggaggccgat ccccacacga tcgcgggcct ggtggccgcg gtcgtgcgga cgctcgggtc
     2401 gacggcgagc gcgccgggct gactcatcga ggtgtccccg aacgcgacga ccgacacggc
     2461 gaccgcgggg gatcgaacat ttcgtcatgt gccctaacct gactgcgtga gctttccgga
     2521 acggcgtctc cgccggctgc ggcgcacgcc cgcgattcgg cgcctcgtgg ccgagcaccg
     2581 gctgaccgtc gacgacctga tcgcgccgct gttcgtcaag gaaggcatcg acgcgcccgc
     2641 gccgatcggg tcgatgccgg gtgtggtgca gcacacgcag gagagcctgc gcaaggaagt
     2701 gcgggcgctc gccgacctcg ggatcccggg cgtcatcctc ttcggtgtcc ccgcgcacaa
     2761 ggacgcgcgg ggctcgcagg ccgacgccga cgacggtgtc gtccaggttg cgctgcgcaa
     2821 cctgcgcgac gacgtcggcg acgagatcgt gctgatggtc gacaactgcc tcgacgagta
     2881 caccgaccac gggcactgcg gcatcctcac cgaccgcggc gacgtcgaca acgacgcgac
     2941 gatcgagcgc tacgcgagca tcgcggtcgc gcaagccgcg gccggcgccg acgtcatcgc
     3001 accgagcggc atgatggacg gccaggtcgg cgcgatccgc accggcctcg acgacgccgg
     3061 cttcgaccag acgagcatcc tcgcctacgc ggccaagttc gcatccgcgc tctacggcc
//
LOCUS       DASUGA010000154         3234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_145240, whole genome shotgun sequence.
ACCESSION   DASUGA010000154 DASUGA010000000
VERSION     DASUGA010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145240"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VFX21_02960"
     CDS             <1..729
                     /locus_tag="VFX21_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5094941.1"
                     /translation="ELFARFGDDLTWVLSLELMNHLEEAQRGVEGMFGYITEFVAERR
                     AAPRDDLVSRLILAEDEGDHLSEDELHMLIGAMLFAGFDTTRNQLGLCMVTFADHPEP
                     WALLAERPELTERAVEECMRFAPTIGIAPRMAAETFEFEGFEIPAGTLVSLSTAAANH
                     DPGAFVEPDVFDITADREPIFTFGGGPHYCLGASLARAEMQVALPLLARRLPDLALAG
                     EVQWRPRMGIFGPTVLPLRFTSAT"
     gene            846..1400
                     /locus_tag="VFX21_02965"
     CDS             846..1400
                     /locus_tag="VFX21_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094942.1"
                     /translation="MKKAFPIALMILGLVFLVAGGYTASRGFDAKDQVRDELVAQNIM
                     TTPDARIPNARVDDAATAKAMADIIDVHAREATGGKTYSEMGRFLATDGTDTNDEALA
                     VKGADGKPVANPLRNTAFQASSLRTSLYSSVMAFNVADLVVGLGVMIAALGLALGGVG
                     VALAGLAIPTFSRRMHVEPVAVAH"
     gene            complement(1469..1855)
                     /locus_tag="VFX21_02970"
     CDS             complement(1469..1855)
                     /locus_tag="VFX21_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX5094943.1"
                     /translation="MAKQKSPSELIDERIEELGDWRGDTLGKVRKIVHDADPDVVEEW
                     KWRGVPTWYHEGIICTGETYKNYVKLTFAKGASLPDPAGLFNSSLEGNVRRAIDIHEG
                     EKIDTRALKALVKAAVALNDSKVKKK"
     gene            1922..2722
                     /locus_tag="VFX21_02975"
     CDS             1922..2722
                     /locus_tag="VFX21_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5094944.1"
                     /translation="MAPVLEVRDDGRLRVLRFNRPERKNALNGQLLRALYAALRAAAT
                     DDDVWALAITGNGDGFCSGLDLDQSNDGGSDEPPRDEQPEHSDGVNTLPILMRVECEK
                     PILAGVNGVAVGLGLSIAMNADIRIAAPSARFHPGYARVATSPDGGLTWTLTRAVGYE
                     RALRFLLEQRMVPAAEALAIGLVSEVVERDEQFEARFLEYGQMLASVAPIAARQTKRM
                     LVKADAPVDLEAHVDEEIALALHGLDSRDSAEAVRAMMAKEKPVFTGE"
     gene            complement(2723..3205)
                     /locus_tag="VFX21_02980"
     CDS             complement(2723..3205)
                     /locus_tag="VFX21_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX5094945.1"
                     /translation="MSAAAEKAFPLFQADVGNDEGTGEWFEVTQDQINQFADVTHDHQ
                     FIHVDPEAAKNTPFGSTIAHGFLTLSMLTHLAKGASSAPADPARYEGIVMGINYGFNR
                     VRFVSPVKVGSRIRARAVTSSVDLKGNMIEVARNFTVEIEGEDKPALVAEWVTRTVYT
                     "
BASE COUNT          509 a         1082 c         1096 g          547 t
ORIGIN      
        1 gagttgttcg cgcgcttcgg tgacgatctc acctgggtgc tgtcactcga gctgatgaac
       61 cacctcgaag aggcacagcg cggtgtcgaa gggatgttcg gatacatcac ggagttcgtc
      121 gccgagcggc gggcggcgcc gcgcgacgac ctcgtctcgc ggctcatcct cgccgaggac
      181 gagggcgacc acctcagcga agacgagttg cacatgctca tcggtgcgat gctcttcgcc
      241 ggcttcgaca caacgcgcaa tcagctcggg ctgtgcatgg tgacgttcgc cgatcatccc
      301 gagccgtggg cgttgctcgc ggaacggccc gagttgaccg agcgcgcagt cgaagagtgc
      361 atgcggttcg caccgacgat cggcatcgca ccgcgcatgg ccgccgagac gttcgagttc
      421 gaggggttcg agatcccggc gggcacgctc gtgtcgctgt cgaccgcggc tgcgaaccac
      481 gacccgggcg cgttcgtcga gcccgacgtg ttcgacatca cggccgaccg cgagccgatc
      541 ttcaccttcg gcggcgggcc gcactactgc ctcggtgcca gcctcgcgcg cgcggagatg
      601 caggtcgcgc tgccgctgct cgcgcgtcgc ctgcccgacc tcgcgctcgc gggcgaggtg
      661 cagtggcgcc cgcgcatggg catcttcgga cccacggtct tgcccctgcg cttcacgtcg
      721 gcgacctgac gtagggacct tcggccctgc gcgcgccgac tatcggccga tgcccgcgag
      781 acccctcgga gcgcaaagta agtgcagccg gtgcactacg ccggcgctct tgagggggta
      841 gatcaatgaa gaaggcattc ccgattgcac tgatgatcct cggtctcgtg ttcctggtcg
      901 cgggcggcta caccgcatcg cgcggattcg acgccaagga ccaagttcgc gacgaactcg
      961 tcgcccagaa catcatgacg acaccggacg cgcgcatccc gaacgcacgt gtcgacgacg
     1021 cggccacggc aaaggccatg gccgacatca tcgacgtgca cgcacgtgag gccacgggcg
     1081 gcaagacgta ctcggagatg ggacgcttcc tggcgacaga cggcaccgac acgaacgacg
     1141 aggcgttggc cgtgaagggt gccgacggca agcccgtcgc caacccgctg cgcaacaccg
     1201 cgttccaggc gtcctcgctg cggacgagcc tgtacagctc ggtcatggcc ttcaacgtcg
     1261 ccgacctcgt cgtcgggctc ggcgtgatga tcgcggcgct cggcctggcg ctcggtggag
     1321 tgggcgtggc cctcgccggt ctggcaatcc cgacgttcag ccgccggatg cacgtcgaac
     1381 cggtggcagt ggcccactga gtccgcaatc ggggcgatcg aaggcgtctg cttcggcagg
     1441 cgccttcgtc gcgtcatgac gcggtccgct acttcttctt gaccttcgag tcgttgaggg
     1501 cgacggcggc cttgacgagc gccttcaatg cgcgcgtgtc gatcttctcg ccttcgtgga
     1561 tgtcgatggc ccgccgcacg ttgccttcga ggctcgagtt gaacagcccg gccgggtcgg
     1621 gcagtgatgc acccttcgcg aacgtcaact tgacgtagtt cttgtaggtc tcacccgtgc
     1681 agatgatccc ttcgtggtac caggtcggca cgccgcgcca cttccactcc tcgacgacgt
     1741 cgggatccgc gtcgtggacg atcttgcgca ccttgccgag cgtgtcgccg cgccagtcgc
     1801 cgagctcctc gatgcgctcg tcgatgagtt cggaagggga cttctgcttc gccatgcggg
     1861 ccacactagg tgtcgaagaa acgcgggtgc ttctccgatc gtgcttggct agcgtgcgtg
     1921 catggctccg gtgctggaag tgcgcgacga cgggcggctg cgggtactcc ggttcaaccg
     1981 ccccgaacgg aagaacgcac tcaacgggca acttctgcgc gcgttgtacg ccgcgctgcg
     2041 cgccgccgca accgacgacg acgtctgggc gctcgcgatc accggcaacg gtgacgggtt
     2101 ctgctccggg ctcgatctcg accagtcgaa cgacggtgga agtgacgagc ccccgcgcga
     2161 cgagcagccc gagcacagcg acggcgtgaa cacgctgccg atcctcatgc gggtcgagtg
     2221 cgagaagccg atcctcgcgg gggtgaacgg cgtcgccgtc gggctcgggc tttcgatcgc
     2281 gatgaacgcc gacatccgca tcgcggcgcc gtccgcgcgc ttccacccgg gctacgcgcg
     2341 cgtcgcgacg tcacccgacg gcggtctcac gtggacgctg acgcgcgccg tcggctacga
     2401 gcgcgcgctg cgtttcctgc tcgaacagcg catggtgccg gccgcggagg cgctggcgat
     2461 cgggctcgtg agcgaggttg tcgagcgcga cgagcagttc gaggctcgct tcctcgaata
     2521 cggacagatg ctggcgagcg tcgcgcccat cgccgcgcgc cagaccaagc gcatgctcgt
     2581 gaaggccgac gcgcccgtcg acctcgaggc acacgtcgac gaggagatcg cgctcgcgct
     2641 gcacggcctc gactcgcgtg acagtgcaga agcggtcaga gcgatgatgg cgaaggagaa
     2701 gccggtcttc accggcgagt gatcacgtgt agacggtgcg cgtgacccac tcggcgacga
     2761 gcgcgggctt gtcttcgcct tcgatctcga ccgtgaagtt gcgggcgacc tcgatcatgt
     2821 tgcccttgag gtcgacgctg ctcgtcaccg cacgcgcacg gatgcgcgaa ccgaccttca
     2881 ccgggctcac gaaccgaaca cggttgaagc cgtagttgat gcccatcacg atgccctcgt
     2941 agcgggcggg atccgccggc gcggagctcg cacccttggc gaggtgcgtc agcatcgaga
     3001 gggtcaggaa gccgtgcgcg atcgtcgagc cgaacggcgt gttcttcgcg gcctcggggt
     3061 cgacgtggat gaactgatgg tcgtgcgtca cgtcggcgaa ctggttgatc tggtcctggg
     3121 tgacctcgaa ccactcgccg gtgccctcgt cgttgccgac gtcggcctgg aacaacggaa
     3181 acgccttctc ggctgctgcg ctcatgcgcc gcaccctaag gcactcgggc gtgg
//
LOCUS       DASUGA010000155         3656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1659690, whole genome shotgun sequence.
ACCESSION   DASUGA010000155 DASUGA010000000
VERSION     DASUGA010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659690"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3285)
                     /locus_tag="VFX21_02985"
     CDS             complement(<1..3285)
                     /locus_tag="VFX21_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HEX5094946.1"
                     /translation="MSPRIRTFVLSFVLASALALPASASANWATIFAAPTSCGVDPGP
                     FSTNAVDTTSSVVPIGWQNSDVAVTLQGTLVDHYEWKLNCGAPSTAGTANIVGDGTWT
                     FTHRAAEAPAGPFTPWVDETVQIDKTVPVNTTATSTAWRTGPFNVPIADLTPDGGSPL
                     HGEWRLDGNPGDPYTTGTAATVPLSAQGTHTLYTSVVDAAGNRADRSQIVRVDNTVPT
                     DDTTAPVGWQQDVAHIVLTGTDAHSGVATIEYQIDSGPVTPVSGNNIPLDITDNGSHT
                     LKTRVLDAVGNDSGWKSQTVQVDSAGPDDATTGVPSGWYNAAPTVSVNVAATDTSGSG
                     VTKIEWNIAEDSRSDTINGATTPPIVISGEGKHTLKVRYTDGMNHVSQWYTHDVWIDT
                     TLPTDTTSAATSWQPLPSLDVTVHGTDAHSGVATVEWKIDGVADSASGDTGTATVAGN
                     GEHTVESRVIDVAGNASAWVSRTVKLDISSPRNTTPVAPTGWISGQYVVLLNGEDDNS
                     GVDSVRWRVDGGAEHVGTRLVETATVAGDQVHTFETRVRDTAGNLSNWRVESVRIDSV
                     KPTNTTIIPGAPVGNGHKIDVDGTDALSGIGGQAWQLDAGAVMTSHQATITGAGPHTL
                     KTRVQDNAGNWSDWRTDSVTVDPALPQEDSDAPLDTTSVPTNWRTGATMITVAADDQG
                     GTGVEYVEWRLDGNAVEQGPSGSTFIVSDDGVHEIETRATDFASNTSAWRSQTLKIDR
                     TLPVDTTSVPASGWINTHSFVLSATDATSGVNEIEYKLDGGVSARVASGFTLNLADGP
                     HTVARRVTDNAGQQTAWKTSTVNVDTSIPANTSATPSTTWVNSAISLPLTGTDTGSGV
                     DHGEWRLGASGTVTSGSAAVVDVDGTSTLQTRVVDKAGNASVWRSDTVKVDLTKPVNT
                     TAAISGGWRKTNFATTVTGSDATSGVQKVEWRVDGAPISATAPVSISADGSHKLETRV
                     TDNAGNVTDWRVDTVAIDKVAPALAVNCGATGVWRRDVVDCSVTADGGVSGMTTLTGT
                     RGGATTDVVNGHFVVDADGAWALSFRAVDGAGNEATGKADVKLDRTAPAAAVTCTPDA
                     ALAYTCM"
     gene            complement(3309..>3656)
                     /locus_tag="VFX21_02990"
     CDS             complement(3309..>3656)
                     /locus_tag="VFX21_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013555504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5094947.1"
                     /translation="RLTEEEFEVVKTHAVLGARVLAGGASDLFAAAESIARSHHERWD
                     GEGYPRGLKGDAIPIAARIVHVADVYDVLVHERPYRESLTPAAAAEEIKRGADTDFDP
                     KVVEVFEKFSLQV"
BASE COUNT          469 a         1209 c         1352 g          626 t
ORIGIN      
        1 catgcaggtg taggcgagtg cggcgtccgg ggtgcaggtg accgcggcgg caggggcggt
       61 gcggtcgagc ttgacgtcgg ccttgcccgt cgcctcgttg ccggcgccgt ccacggcgcg
      121 gaagctcagc gcccacgcgc cgtcggcgtc gaccacgaag tggccgttga ccacgtcggt
      181 cgtcgctccg ccgcgggtgc cggtcagcgt ggtcatgccc gagacgccgc cgtcggcggt
      241 gacggagcag tcgacgacgt cgcgccgcca gacgccggtg gcgccgcagt tgaccgcgag
      301 ggccggagcg accttgtcga tcgcgacggt gtcgacgcgc cagtcggtga cattgccggc
      361 gttgtcggtc acgcgcgtct cgagcttgtg cgacccgtcg gcgctgatcg acacgggggc
      421 ggtcgcggag atgggcgcgc cgtccacgcg ccactcgacc ttctgcacac ccgaggtggc
      481 gtccgagccg gtgaccgtcg tcgcgaagtt cgtcttgcgc cagccgcccg agatcgcggc
      541 ggtcgtgttg accggcttgg tcaggtcgac cttgaccgtg tccgagcgcc agacagaagc
      601 gttgccggcc ttgtcgacca cgcgcgtctg cagcgtggac gtgccatcga catcgaccac
      661 cgccgcagag ccgctcgtca cggtcccaga cgcgcccagg cgccactcgc cgtggtccac
      721 gcccgagccc gtgtcggtcc cggtcagcgg cagcgagatc gcgctgttga cccacgtcgt
      781 cgaaggcgtc gcggacgtgt tcgcagggat cgacgtgtcg acgttgaccg tggaggtctt
      841 ccatgcggtt tgctggccgg cgttgtcggt gacgcggcgg gccacggtgt gcgggccgtc
      901 agcgaggttg agcgtgaagc ccgacgcgac gcgggccgag accccaccgt cgagcttgta
      961 ctcgatctca ttcacgcccg aggtcgcgtc ggttgcgctc aggacgaagc tgtgcgtgtt
     1021 gatccagccg ctggccggca ccgacgtggt gtccacgggc agcgtgcggt cgatcttcag
     1081 tgtctgggaa cgccaggcgc tcgtgttgga ggcgaagtcc gtggcccgcg tctcgatctc
     1141 gtgcacgccg tcgtcgctga cgatgaacgt ggagccgctc gggccctgct cgaccgcgtt
     1201 gccgtccagc cgccactcga cgtactccac gccggtgccg ccctggtcgt cggccgcgac
     1261 cgtgatcatc gtcgcgcccg tgcgccagtt ggtcgggacg ctggtcgtgt ccagcggcgc
     1321 gtcggagtcc tcctgcggaa gcgccggatc gacggtcacc gagtcggtgc gccagtcgct
     1381 ccagttgccc gcgttgtcct gcacgcgggt cttcagggtg tgcgggccgg cgccggtgat
     1441 cgtcgcctgg tgagacgtca tgaccgcgcc ggcgtcgagc tgccacgcct ggccgccgat
     1501 gcccgacagc gcgtcggtgc cgtcgacgtc gatcttgtgg ccgttgccca ccggagcgcc
     1561 ggggatgatc gtcgtgttcg tcggcttgac ggagtcgatg cgcacgctct cgacccgcca
     1621 gttggacagg ttgcccgccg tgtcgcggac gcgcgtctcg aacgtatgga cctggtcacc
     1681 cgcgaccgtc gcggtctcga ccaggcgcgt gccgacgtgc tcggcgccgc cgtcgacgcg
     1741 ccagcggacg gagtcgacgc cggagttgtc gtcctcgccg ttcaagagca ccacgtactg
     1801 accggagatc cagccggtcg gagccaccgg ggtcgtgttg cgcggcgacg agatgtccag
     1861 cttcacggtg cgcgagaccc acgcggaggc gttgcccgcg acgtcgatga cgcgcgattc
     1921 gacggtgtgc tcgccgttgc cggcgacggt cgcggtgccc gtatcaccgg acgcggaatc
     1981 cgcgacgccg tcgatcttcc actcgaccgt ggccacgccg gagtgggcgt cggtgccgtg
     2041 cacggtcacg tcgagcgagg gcagcggctg ccagctggtc gccgcgctgg tcgtgtcggt
     2101 cggcagggtg gtgtcgatcc agacgtcgtg cgtgtaccac tgcgagacgt ggttcatgcc
     2161 gtccgtgtaa cggaccttca aggtgtgctt gccctcgcca ctgatcacga tcggcggcgt
     2221 ggtggcgccg ttgatcgtgt ccgagcgcga gtcctcggcg atgttccact cgatcttggt
     2281 cacgcccgag cccgaggtgt cggtcgccgc gacgttgacg ctgacggtcg gagcggcgtt
     2341 gtaccagccg ctcgggacgc cggtcgtggc gtcgtccggg ccggcggagt cgacctgcac
     2401 ggtctgggac ttccaacccg agtcgttgcc gacggcgtcg agcacccgcg tcttgagcgt
     2461 gtgcgagccg ttgtcggtga tgtcgagcgg gatgttgttg ccgctgaccg gggtcaccgg
     2521 gccgctgtcg atctggtact cgatcgtcgc gacgcctgag tgggcgtcgg tgccggtgag
     2581 cacgatgtgc gcgacgtcct gctgccagcc gacgggagcc gtcgtgtcgt cggtcgggac
     2641 ggtgttgtcc acgcggacga tctgcgagcg gtcggcgcgg ttgcccgcgg cgtcgaccac
     2701 cgacgtgtag agcgtgtggg tgccctgggc ggagagcggg acggtcgcgg cggtgccggt
     2761 ggtgtaggga tcgccggggt tgccgtcgag gcgccattcg ccgtgcagcg gcgagccgcc
     2821 gtcgggggtc aggtccgcga tcggcacgtt gaacgggccg gtgcgccagg ccgtcgaggt
     2881 cgcggtcgtg ttgaccggca cggtcttgtc gatctgcacc gtttcgtcga cccacggggt
     2941 gaacgggccg gcgggcgcct ctgcggcgcg gtgcgtgaag gtccaggtgc cgtcaccgac
     3001 gatgttcgcg gtgccggcgg tcgagggcgc gccgcaattc agcttccact cgtagtggtc
     3061 cacgagcgtg ccctgcaggg tgacggcgac gtcgctgttc tgccagccga tcgggacgac
     3121 gctcgacgtg gtgtcgacgg cattcgtact gaacggaccc ggatccacac cgcacgacgt
     3181 gggtgcggcg aagatcgtcg cccagttcgc ggacgcggac gcgggcaagg ccagtgccga
     3241 ggcgagaacg aacgagagga cgaatgtgcg aatccgaggg ctcacgcccc ttttatcggc
     3301 aagccgattc agacctgcag tgagaacttt tcgaagacct cgacaacctt cggatcgaag
     3361 tcggtgtccg cgccgcgctt gatctcctcg gcggcggccg ccggggtgag cgactcgcgg
     3421 tacggacgtt cgtgcacgag cacgtcgtag acgtcggcca catgcacgat gcgcgcggcg
     3481 atcgggatcg cgtcgccctt cagcccgcgc gggtagccct cgccgtccca gcgctcgtgg
     3541 tggctgcggg cgatcgactc cgccgcggcg aacagatccg acgcgccgcc tgcaagaact
     3601 cttgcaccga gcacggcatg ggtcttgacg acctcgaact cctcctcggt gagccg
//
LOCUS       DASUGA010000156         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1743494, whole genome shotgun sequence.
ACCESSION   DASUGA010000156 DASUGA010000000
VERSION     DASUGA010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1743494"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VFX21_02995"
     CDS             complement(<1..266)
                     /locus_tag="VFX21_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013424550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEX5094948.1"
                     /translation="MATSFPDGFLWGTATAAHQIEGANWNCDWWAFEHDPGSGCVEPS
                     GDACDSFHRYGQDIALVRDMGLGAYRFSIEWARIEPEDGEFSVAA"
     gene            294..1100
                     /gene="trpC"
                     /locus_tag="VFX21_03000"
     CDS             294..1100
                     /gene="trpC"
                     /locus_tag="VFX21_03000"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013349292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX5094949.1"
                     /translation="MSVLDEILATKRDEVTVLRQPQTRDLLRRMALDAAPARDFAGAL
                     RRADGHLALIGELKRRSPSKGFLAPDLDPAPTAKAYEAGGAGALSVLTDLPYFGGTVD
                     DLQAAHAATELPVLRKDFTIDEIQVYEARAIGADAVLLIAAALPDDSLFADLHALAVD
                     LGLGVLVETHDEAEIGRAVAAGARIVGVNSRNLATFDEDLGIAERLSALLPPDAVAVA
                     ESAVRSAGDAERMAHAGFDAVLVGEALVKAPDATALVRAMTGATVARRDR"
     gene            1104..1805
                     /locus_tag="VFX21_03005"
     CDS             1104..1805
                     /locus_tag="VFX21_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX5094950.1"
                     /translation="MFVKICGITNEEDALLATALDADALGFVFASGSRRQVKPDLVRD
                     IVKRLPGEVLTVGVFRNERPERVVEITNRVGLHAVQLHGNEPLSEVRYIRERVQFVIQ
                     AFAAGDPNLGAAENSPADVVLIDNADPGSGKVFDWRLAEGAPRGVKLLLAGGLTPDNV
                     GDAIRLVRPWGVDVSSGVEASPGHKDPRKLRRFVEAARGADQDPIDLTGEPLVHRDDD
                     EVAGVSRPWDWQLDD"
     gene            1833..>2182
                     /locus_tag="VFX21_03010"
     CDS             1833..>2182
                     /locus_tag="VFX21_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014440484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5094951.1"
                     /translation="MAVPARGEFVTLGPPGPEPTGLGRFEEYGGRFVPETLIAALEQL
                     EAEFRTAWHDEAFRQEYASLLASYAGRPTPVTECHRLSERLGVRVLLKREDLTHTGSH
                     KINNVLGQALLTRR"
BASE COUNT          334 a          762 c          748 g          338 t
ORIGIN      
        1 gcggcgacgg agaactctcc gtcttcgggt tcgatgcgcg cccattcgat cgagaaccgg
       61 tacgcgccga gtcccatgtc gcgcaccagc gcgatgtctt gtccgtaacg gtggaagctg
      121 tcgcaggcat cgcccgaggg ctcgacgcat ccgctcccgg gatcgtgctc gaacgcccac
      181 cagtcgcagt tccagttggc cccttcgatc tggtgcgccg cagtcgcggt gccccacaag
      241 aagccgtcgg gaaagctcgt cgccatgggc ccggagcgta gacttgaccc tcggtgagtg
      301 ttctcgacga gattctcgcg acgaagcgcg acgaggtcac ggtgctccgc cagccgcaga
      361 cgcgtgatct gttgcggcgc atggcgctcg acgccgcgcc ggcgcgtgac ttcgccggcg
      421 cgctacgacg cgccgacggt catctcgcgt tgatcggcga gctgaagcga cgttcgccgt
      481 cgaaggggtt cctcgccccc gacctcgacc ccgcgccgac cgcgaaggcg tacgaggcgg
      541 gcggcgcggg cgcgttgtcg gtgctcaccg acctgccgta cttcggcggc accgtcgacg
      601 acttgcaggc ggcgcacgcc gccaccgagc tgccggtgct gcgcaaggac ttcacgatcg
      661 acgagatcca ggtctacgaa gcccgcgcga tcggcgccga tgcggtcttg cttatcgcgg
      721 ctgcgctccc ggacgactcg ctgttcgccg acttgcacgc ccttgccgtc gacctcgggc
      781 tcggcgtgct cgtcgagacg cacgacgaag ccgaaatcgg gcgcgcggtc gcagccggcg
      841 cccgcatcgt gggtgtgaac agccgcaacc tcgcgacgtt cgacgaagac ctcgggatcg
      901 cggaacggct gtcggcgctg ctgcccccgg acgcggtcgc ggtggccgag agcgcggtgc
      961 gctccgccgg cgatgccgaa cgcatggcgc acgcggggtt cgacgcggtg ctcgtcggtg
     1021 aggcactcgt caaggccccc gacgcgaccg cattggtgcg cgcgatgacc ggcgcaacag
     1081 tcgcgcggcg agatcgctga cccatgttcg tcaagatctg cggcatcacg aatgaggaag
     1141 atgcgctcct cgcgaccgcg ctcgacgcgg acgcactcgg cttcgtcttc gcgtcgggaa
     1201 gccgccggca ggtgaaaccc gatctcgtgc gcgacatcgt caaacgattg ccgggcgagg
     1261 tgctcaccgt gggtgtgttc cgcaacgaac gccccgaacg agtcgtcgag atcacgaacc
     1321 gcgtcggttt gcacgcggtg cagctgcacg gcaacgagcc gttgtcggaa gtgcggtaca
     1381 tccgcgaacg tgtgcagttc gtcatccagg cgttcgcggc cggcgacccc aacctcggcg
     1441 cggccgagaa ctcgcccgcc gacgtggtcc tcatcgacaa cgccgatccg ggctcgggca
     1501 aggtgttcga ctggcggctc gcggagggtg cgccgcgcgg cgtgaagctg ttgctcgccg
     1561 gcgggctcac acccgacaac gtcggcgacg cgatccggct cgtccgaccg tggggtgtcg
     1621 acgtgtcgag cggagtcgaa gcaagcccgg gccacaagga tccgcgtaag ttgcggcgct
     1681 tcgtcgaggc cgcgcgcggc gcggaccagg atccgatcga cctcaccggc gaaccgctcg
     1741 tacaccgcga cgacgatgag gtcgccggtg tctcccgacc gtgggattgg cagctcgacg
     1801 actgagggtt gccctctatc atctgtcgac ttatggccgt ccccgctcgc ggtgagttcg
     1861 tcacgctcgg cccgcccggc cccgaaccga ccgggctggg gcgcttcgag gagtacggcg
     1921 gccgcttcgt ccccgagacg ctgatcgcag cgctcgagca gctcgaagcc gagttccgca
     1981 cggcttggca cgacgaagcg ttccggcagg agtacgcatc gctgctcgcc tcgtacgccg
     2041 gccggccgac cccggtgacc gagtgccacc ggctgtcgga gcgcctcggt gtgcgggtgc
     2101 tcctcaagcg cgaagacctc acccacaccg ggtcccacaa gatcaacaac gtgctcgggc
     2161 aggcactgct cacgcgccgt at
//
LOCUS       DASUGA010000157         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_648354, whole genome shotgun sequence.
ACCESSION   DASUGA010000157 DASUGA010000000
VERSION     DASUGA010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648354"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VFX21_03015"
     CDS             <1..289
                     /locus_tag="VFX21_03015"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX5094952.1"
                     /translation="TELSAEGCTLQVQSQMWSGDSGRMRADALGEVWFPVRVTRVRRS
                     TPADWTVTVQFDRLTDEKRQLVKELILRENRRVLEQFATKRSVGSGTVPSA"
     gene            complement(315..388)
                     /locus_tag="VFX21_03020"
     tRNA            complement(315..388)
                     /locus_tag="VFX21_03020"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(352..354),aa:Val,seq:cac)
     gene            complement(432..1628)
                     /locus_tag="VFX21_03025"
     CDS             complement(432..1628)
                     /locus_tag="VFX21_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX5094953.1"
                     /translation="MGTFSAEELLADADRVLPDAIALRREIHRTPELGLELPRTQELV
                     LDALNGFDLDVSTGTGVSSVTADLETGDGPVVLLRGDMDALPMPEDTGLDFASRVDGC
                     MHACGHDAHTAMLVGAVRLLHDRRAELPGVVRFMFQPGEEGFHGARHMIDEGVLAGER
                     DVDAAFALHVSPNLPSGTIWTRGGPLMASADVLSVTVTGKGGHASTPYLANDPMPVAA
                     EIVQALQVMVTRRINTFDPIVVTITKIRAGTTNNVIPECVKMEGTLRAVSEHSRTQAI
                     DAMHRLVDGIAGAHDMTAEMHVEPGYPVTVNHEDDATFALRVAGELLGADHTGRMPAP
                     VMGAEDFSYVLQQKPGAMAFLGVCPPGEHPARAHACHSNRMTLDEDAMRAGIAMYAAL
                     ALQYGT"
     gene            complement(1643..>2093)
                     /locus_tag="VFX21_03030"
     CDS             complement(1643..>2093)
                     /locus_tag="VFX21_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094954.1"
                     /translation="AARSAGDRAALRDAMRHLRYAELRDGVWVRPDNLPRASAADEWW
                     AIADEQCSWWSATPDDDPGDLFASDAWATRAASLTARLAEATSGLDDDRLADAFVTGA
                     AALAHIRSDPLLPAELVDPGPGTELRSVYRAYETAFSGAITSWFRAH"
BASE COUNT          350 a          742 c          682 g          319 t
ORIGIN      
        1 caccgagctc tccgcggaag gttgcacgct tcaggtccaa agccaaatgt ggtccgggga
       61 ttcaggccgg atgcgcgccg acgcgctcgg cgaagtgtgg ttccccgtcc gcgtcacgcg
      121 cgtgcgacgg tcgacgccag ccgactggac cgtgaccgtc cagttcgacc gcctcaccga
      181 cgaaaagcgc caactcgtca aggaactcat tctgcgcgag aaccgccgcg tcctcgaaca
      241 gttcgccacg aagcggtccg tcggatccgg gaccgtcccg tcagcgtgaa tgatgaagga
      301 gccaggctca cgagtgggcg gtaagggact cgaacccccg acctcatccg tgtgaaggat
      361 gcgctctagc caactgagct aaccgccccg cgagcgggac actctagcgg cgacccggga
      421 tgggctccgc tctaggttcc gtactgcaac gccagcgcgg cgtacatcgc gattccggcg
      481 cgcatcgcat cttcgtcgag cgtcatccgg ttcgagtgac acgcgtgcgc acgcgcggga
      541 tgttcacccg gcggacacac cccgaggaaa gccattgcgc ccggcttttg ttgcaacacg
      601 tacgagaagt cctcggcgcc catcaccggt gccggcatgc ggcccgtgtg gtcggcaccg
      661 agcaattcac ccgcgactcg caacgcaaag gtcgcgtcgt cctcgtggtt cacggtgacc
      721 gggtaacccg gctcgacgtg catctcggca gtcatgtcgt gcgcgccggc aattccatcc
      781 accagacggt gcatcgcgtc gatggcctgc gtccggctgt gctcggacac ggcgcgcagc
      841 gtgccttcca tcttcacgca ctccgggatc acgttgttcg tcgtgcccgc gcggatcttg
      901 gtgatcgtca cgacgatcgg gtcgaacgtg ttgatccggc gcgtgaccat gacctgcagc
      961 gcttgcacga tctcggccgc gaccggcatc ggatcgttgg caaggtacgg cgtcgatgca
     1021 tgaccaccct tgccggtcac cgtcaccgac agcacgtcgg ccgatgccat cagcgggccg
     1081 ccgcgcgtcc agatcgtccc cgacggcagg ttcggcgaca cgtgcaaggc aaacgcggcg
     1141 tcgacgtcgc gctcccccgc gagcacgcct tcgtcgatca tgtggcgagc gccgtggaat
     1201 ccctcttcgc cgggctggaa catgaagcgc acgacacccg gcagctcggc ccgccgatcg
     1261 tgcagcaaac ggaccgcgcc aaccagcatt gcggtgtgcg cgtcgtgccc gcacgcatgc
     1321 atgcagccgt cgacgcgact cgcgaagtcg aggccggtgt cctccggcat gggtagcgcg
     1381 tccatgtcgc cgcgcagcaa cacgaccggg ccgtcgccgg tctcgagatc ggcggtgacc
     1441 gacgagacgc cagtaccggt cgagacgtcg aggtcgaacc cgttcagcgc gtcgagcacc
     1501 agctcctgcg tacgcggcag ttcgagcccc agctccggcg tgcggtggat ctcgcggcgc
     1561 aacgcgatcg cgtccggtaa cacgcggtca gcatccgcga gcaattcctc cgccgagaac
     1621 gtacccacgc gcgaacagta actcagtgag cccgaaacca gctcgtgatc gcacccgaga
     1681 acgcggtctc gtaggcgcga tacaccgaac ggagctccgt tcctggcccg gggtcgacca
     1741 gctcggccgg caacagcggg tcggatcgaa tgtgggcgag cgccgccgcg ccggtgacga
     1801 acgcatcggc cagccggtcg tcgtcgagcc cgctggtcgc ttcggccaga cgcgcggtca
     1861 gactcgccgc gcgcgtcgcc caggcatccg acgcgaacag gtcgcccgga tcgtcgtcgg
     1921 gcgttgccga ccaccacgaa cactgttcgt cggcgatcgc ccaccattcg tcggcagccg
     1981 acgcacgcgg caggttgtcg ggccggaccc agacgccgtc gcgcagttcg gcgtagcgga
     2041 ggtgccgcat ggcatcgcgc agtgcggccc ggtcgccggc gctgcgcgcc gcc
//
LOCUS       DASUGA010000158         2995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2058025, whole genome shotgun sequence.
ACCESSION   DASUGA010000158 DASUGA010000000
VERSION     DASUGA010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2058025"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VFX21_03035"
     CDS             <1..360
                     /locus_tag="VFX21_03035"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5094955.1"
                     /translation="METEVAGLPLYPDATNLHNPDFAAYAHACGAGGVRVEHARDIVP
                     AIEAAIAYDGPFLIDAVVSPGELTMPPHLDFEMAWGFGLSKAKEALIGVRGEHEQWKG
                     WRDELKANLHKESFDAL"
     gene            complement(412..2247)
                     /gene="nhaA"
                     /locus_tag="VFX21_03040"
     CDS             complement(412..2247)
                     /gene="nhaA"
                     /locus_tag="VFX21_03040"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX5094956.1"
                     /translation="MAVSGITRAENRLTAVRAFAATENAGAVVLLAATVAALVWANSP
                     WSATYTELWDTEFGLSFGDHTLVMDLRDWVNDGLMTLFFFVAGLEIRRELDMGDLREH
                     RRVAVPVIAAVGGMAMPALIYLAFNAGRSSADGWGIAMGTDTAFAIAVLGLVGGATPR
                     ARTFLLSLVVVDDAIALTIVAVFYSSDLALKPLLIGAAFYGVVFILKRAGVLNGVAYF
                     LVGLGMWFAFVESGVHPTIVGVLIGLLATAYPPSRRELERAARNWRVFREEPSPEAAR
                     IASRTVTYSVSPNERLQHAYHPWTSFLIVPLFALANAGVDISAQRLRDAATSPITLGI
                     MVGLVVGKTVGITGAAWLARRVGKLPITVPWSSVFGTSTVAGIGFTVSLLIAGITFEG
                     DDLVEATLGVLGASVLAAALSWVVFRVVRRLPARTANAGVAPTLGDLSEQVDPEVDHL
                     HGSVDAPVVLVEYGDFECPHCLAAEGIITQFKTEFAGQLAYVFRHLPLDDVHEHARLA
                     AEAAEAAGAQGKYWEMHKVLFAHQDALLEPDLVQYATDLDLDVERFLADLHNRKYALR
                     VQRDIVSADDSGAVGTPTFFVNGRRHQGNIDVASLAESVRLALTE"
     gene            2418..2490
                     /locus_tag="VFX21_03045"
     tRNA            2418..2490
                     /locus_tag="VFX21_03045"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2451..2453,aa:Arg,seq:acg)
     gene            complement(2528..2704)
                     /locus_tag="VFX21_03050"
     CDS             complement(2528..2704)
                     /locus_tag="VFX21_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEX5094957.1"
                     /translation="MTDGNVANAEQREQRLAPAMAYYIEHFYNSARRHSALSYLTPNE
                     FEAIHSAKTPATSS"
     gene            complement(2721..>2995)
                     /locus_tag="VFX21_03055"
     CDS             complement(2721..>2995)
                     /locus_tag="VFX21_03055"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007262109.1"
                     /note="Catalyzes the reversible hydration of unsaturated
                     fatty acyl-CoA to beta-hydroxyacyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX5094958.1"
                     /translation="RLTAPGDALRAAIELAHEIALFPQGALRCDRRSVLEQWSLPELD
                     AVANEVDLAMAVYEHDPVAHEQGVAVFESGVGRHGRLADGRLMTDL"
BASE COUNT          521 a          973 c         1052 g          449 t
ORIGIN      
        1 atggagaccg aagtcgcggg tctcccgctc tatccggacg cgacgaacct ccacaacccc
       61 gacttcgcgg cgtacgcgca cgcgtgcggc gcgggcgggg tgcgcgtcga acacgcgcgt
      121 gacatcgtgc ccgcgatcga ggccgcgatc gcgtacgacg gtccgttcct catcgacgcg
      181 gtcgtcagcc ccggcgagct gacgatgccg ccgcatctcg acttcgagat ggcatgggga
      241 ttcgggctct cgaaggcgaa agaagcgctc atcggcgtgc gcggcgagca cgagcagtgg
      301 aaaggctggc gcgacgagct caaagccaat ctccacaagg agagcttcga cgcgctctga
      361 ggaagcgggt gttcagggca ggcggcggcg cagcatcgga gcccgaacgc gtcactccgt
      421 cagggccagt cgtaccgact cggcgagcga cgcaacgtcg atgttgccct ggtggcgccg
      481 gccgttgacg aagaacgtgg gcgtgcccac cgcgccgctg tcgtcggcac tcacgatgtc
      541 gcgctgcacg cgcagggcgt acttgcggtt gtgcaggtcg gcgaggaagc gctcgacgtc
      601 gaggtcgaga tcggtcgcgt attgcacgag atcgggttcg aggagcgcgt cttggtgcgc
      661 gaacaagacc ttgtgcatct cccagtactt gccctgtgcg cccgcggcct cggcggcttc
      721 ggccgcgagg cgcgcgtgct cgtgcacgtc gtcgagcggc aggtgccgga agacgtaggc
      781 cagctgcccg gcgaactcgg tcttgaactg ggtgatgatt ccctcggccg ccaggcagtg
      841 cgggcattcg aagtcgccgt actcgacgag gacgaccggc gcgtcgaccg atccgtgcag
      901 gtggtcgact tccgggtcca cctgctcgga cagatcgccg agagtcggag caacgcccgc
      961 gttcgcggta cgggccggga gccggcgtac gacccggaag acgacccacg acagcgcggc
     1021 cgccaacacc gacgctccga gcacgccgag ggtcgcctcg accaggtcgt caccttcgaa
     1081 ggtgatgccc gcaatgagca acgagacggt aaagccgatc ccggccaccg tcgacgtgcc
     1141 gaagaccgac gaccacggca ccgtgatcgg cagtttgccg acgcgccgcg cgagccacgc
     1201 ggcgccggtg atgccgacgg ttttgccgac gacgagaccg accatgatgc ccagcgtgat
     1261 cggcgacgtc gcggcgtcgc gcagccgctg cgcgctgatg tcgacgccgg cgttggcgag
     1321 cgcgaacaac gggacgatga gaaagctggt ccacgggtgg tacgcgtgtt gcagccgttc
     1381 gttcggtgac acgctgtagg tgacggtgcg gctcgcgatg cgggcggcct cgggacttgg
     1441 ttcctcacgg aagacgcgcc agttgcgcgc cgctcgttcc aactcgcgcc gcgacggtgg
     1501 atacgcagtc gcaagcagtc cgatgagtac gccgacaatt gtcggatgca ctcccgactc
     1561 gacgaatgcg aaccacatgc cgagaccgac gagaaagtac gcgacgccgt tgagaacgcc
     1621 cgcgcgcttg aggatgaaga cgacgccgta gaacgcggct ccgatcaaca gcggcttcag
     1681 ggcgagatcg ctgctgtaga agacggcgac gatcgtcagc gcgatggcgt catcgaccac
     1741 gacgagactg aggagaaacg tgcgggcgcg cggcgtcgcg ccgccgacga ggccgagcac
     1801 tgcgatcgca aaggccgtgt cggtgcccat cgcgatgccc catccgtcgg ccgaggagcg
     1861 gccggcgttg aaggccaagt agatgagtgc gggcatcgcc atgccaccga cggcggcgat
     1921 gacaggcacg gcgacgcggc gatgctcgcg aaggtcgccc atgtcgagct cgcgacggat
     1981 ttcgagtccc gcgacgaaga agaagagcgt catcagtccg tcgttgaccc agtcccgcag
     2041 gtccatgacc agggtgtgat cgccgaacga cagcccgaac tcggtgtccc agagttcggt
     2101 gtacgtggcc gaccacggtg agttcgccca cacgagtgcg gcgaccgtcg cggcgagcaa
     2161 caccaccgcg cccgcgttct cggtcgccgc gaacgcgcgc accgcggtca ggcgattctc
     2221 cgcacgggtg atgccggaaa cggccacgac cgatcagcgt ggcggcaccg gcgatggacc
     2281 gcaacttggg cgcggtgggt cggcgcgcac gtccggcccc ggcgctcaca gccggcacgt
     2341 cgcaagattc gccccggcgg gcgccgagaa cacaacggga tcagacccgg cgacttcccc
     2401 tacactgtct cggcccagcg ctcgtagctc aatggataga gcatctgact acggatcaga
     2461 aggttggggg ttcgaatccc tccgagcgcg cgctgtgaag ccttgacccc attgggtgga
     2521 ccagtcgtta tgacgacgtg gccggggtct tggctgaatg tattgcttcg aactcgttcg
     2581 gggtgaggta gctgagcgca ctgtggcgcc gggcggagtt gtagaagtgc tcgatgtagt
     2641 aggccattgc cggggcgagt cgctgctcac gctgttcggc gttcgcgacg ttgccatcag
     2701 tcacgcgtca cggctcctgc tcagaggtcg gtcatcaatc ggccgtcggc gaggcggcca
     2761 tgccgcccga cgccggactc gaatacggca acgccctgct cgtgcgcgac cgggtcgtgc
     2821 tcgtagacgg ccatcgcgag gtcgacctcg ttggcgaccg cgtcgagctc gggcaggctc
     2881 cactgctcga gcaccgaacg gcgatcgcag cgcagcgcgc cctgcgggaa cagcgcgatc
     2941 tcgtgggcca gctcgatcgc cgcgcgcagt gcgtctccgg gtgccgtcag ccgtt
//
LOCUS       DASUGA010000159         2059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_493269, whole genome shotgun sequence.
ACCESSION   DASUGA010000159 DASUGA010000000
VERSION     DASUGA010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493269"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VFX21_03060"
     CDS             <1..206
                     /locus_tag="VFX21_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEX5094959.1"
                     /translation="PLGIDVSCVGVAQAYAAFCSSLVIDAVDAARAPEVEALGVRAVV
                     TDTMMRSPEIAAALAAETLAAVA"
     gene            203..910
                     /gene="cofE"
                     /locus_tag="VFX21_03065"
     CDS             203..910
                     /gene="cofE"
                     /locus_tag="VFX21_03065"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX5094960.1"
                     /translation="MTLTVIPITGMGEIRPGDELAVFIASAATEQGTPLADGDCLVVT
                     QKVVSKAENRLVPLDPDDLAARRELVESESVRIVRRRGDLIISETRHGFVCANAGVDL
                     SNVEHGWAALLPVDGDRSAKHIRDALRARAGVDVGVIISDTFGRPWRQGLTDVAIGVS
                     GIAAVVDLRETPDALGRELHVTEVAVADEISSAAELVMGKAAGVPAAIVRGLDGSWFR
                     DGSVRELVRPTQDDLFR"
     gene            907..1512
                     /locus_tag="VFX21_03070"
     CDS             907..1512
                     /locus_tag="VFX21_03070"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX5094961.1"
                     /translation="MSVPAFLEARRSIRAFLPKTVARDDIDVLVEAACIAPAPHHSRP
                     WRFVVLDTADAKQRLADGMGEKWRTDLAGDDVDTARIDELVAKSHEKITGAPVVVLGC
                     LTWDGLDRYPDEKRQRAEWGMALLSLGAAVENFMVAAADRGLASCWVAAPIFCPEEAR
                     DALALPPEWLPHAMVVVGYPDPAYVGRPRPPIPLEDLRRFL"
     gene            complement(1553..>2059)
                     /locus_tag="VFX21_03075"
     CDS             complement(1553..>2059)
                     /locus_tag="VFX21_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094962.1"
                     /translation="LASIYKSPQFIETVLHAMLRKKLMTIESTVAVFRRLARRGRPGI
                     AVTRAVLEQFDPSLALTESPPETKLLHILRRGGLGRVIPQMEIRDEQGNFVARVDIGL
                     PDMGATVEYDSDQEHGDVISIGRDNERRNRIFKAKWTPFVARRRDLATNCVALIQAIK
                     AHSHSQPA"
BASE COUNT          306 a          709 c          693 g          351 t
ORIGIN      
        1 acccgctcgg catcgacgtc tcgtgtgtcg gcgtcgcgca ggcgtacgcc gcgttctgca
       61 gctcgctcgt catcgacgcc gtcgatgcgg cgcgcgcgcc cgaggtggag gcgctcggcg
      121 tacgcgcggt cgtcaccgac acgatgatgc gcagtcctga gatcgcggcc gcgctcgccg
      181 ccgagacgct cgccgccgtc gcgtgacgct caccgtcatc ccgatcaccg gcatgggcga
      241 gattcgtccg ggtgacgagc tcgccgtgtt catcgcgagc gccgcgaccg agcagggaac
      301 gccgctcgcc gacggcgact gcttggtcgt gacgcagaag gtcgtgtcga aagcagaaaa
      361 ccggttggtg cccctcgatc ccgacgatct cgctgcgcgc cgcgagctgg tcgagtcgga
      421 gtcggtgcgc atcgtgcgcc gtcgcggcga cctcatcatc agcgagacgc gccacggctt
      481 cgtctgcgcg aacgcgggcg tcgatctctc gaacgtcgag cacggctggg ccgcgctgct
      541 gcccgtcgac ggtgaccggt ccgccaagca cattcgcgac gcgctccggg cccgcgccgg
      601 ggtcgacgtg ggcgtgatca tctccgacac cttcggacgc ccgtggcggc agggcctcac
      661 cgacgtcgcc atcggcgtga gcggcattgc ggccgtcgtc gacctgcgcg agacccccga
      721 cgcattgggg cgcgagctgc acgtcaccga ggtggcggtc gccgacgaga tctcgtccgc
      781 agccgaactc gtgatgggca aagccgccgg cgtgccggcc gcgatcgtgc gggggctcga
      841 cgggtcgtgg ttccgcgatg gctcggtgcg ggaactggtg cggccgacgc aggacgactt
      901 gttccgatga gcgtgcccgc gtttctcgag gcgcgccgct caatacgcgc gttcctaccg
      961 aagacggtcg cgcgcgacga catcgacgtg ctcgtcgaag cggcgtgcat cgcgcccgcg
     1021 ccgcatcact cgcggccgtg gcggttcgtc gtgctcgata cggccgacgc gaagcagcgc
     1081 ctcgccgacg gcatgggcga gaagtggcgt accgacttgg ccggcgacga cgtcgatacc
     1141 gcgcgcattg acgaattggt ggcgaagtcc cacgagaaga tcacgggcgc accggtcgtc
     1201 gtgctcgggt gtctcacgtg ggacggactc gatcgctatc ccgacgagaa gcgccagcgc
     1261 gccgaatggg gcatggcgtt gctctcgctc ggcgctgccg tcgagaactt catggtcgcg
     1321 gccgccgacc gcggtctcgc gtcgtgctgg gtcgcggccc cgatcttctg ccccgaggaa
     1381 gcgcgcgacg cgctcgcgct cccgcccgag tggctccccc acgcaatggt cgtcgtcggc
     1441 taccccgacc cggcgtacgt cggccgcccc cgcccaccca tccccttaga ggatcttcgc
     1501 cgcttcctct gatcgaactt gcgtgagaaa cggccccgtc agggcgtgct tctcacgccg
     1561 gttgggagtg ggaatgcgcc ttgatcgcct ggatcagtgc aacgcagttc gtcgcgaggt
     1621 cccgtcgccg cgcgacgaac ggggtccact tcgccttgaa gattcggttt cggcgctcgt
     1681 tgtctcgacc aatcgagatc acatcgccgt gctcttgatc actgtcgtac tcgaccgtcg
     1741 cacccatatc cggcaacccg atgtccacac gagcgacgaa gtttccctgc tcgtcgcgta
     1801 tctccatctg cggaataacc cgaccgagac ctcctcggcg cagtatgtgg agcagcttcg
     1861 tttcgggggg cgattccgtg agcgcgagcg acgggtcgaa ctgctcgagc actgcacgag
     1921 tgaccgctat ccccggccgg cctcggcgag caagcctgcg gaacactgcg accgtcgatt
     1981 cgatcgtcat caatttcttg cgaagcatcg cgtgcaaaac ggtttcgatg aactgcggcg
     2041 acttgtagat ggatgcgag
//
LOCUS       DASUGA010000160         3234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1116908, whole genome shotgun sequence.
ACCESSION   DASUGA010000160 DASUGA010000000
VERSION     DASUGA010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116908"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..2621
                     /locus_tag="VFX21_03080"
     CDS             264..2621
                     /locus_tag="VFX21_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5094963.1"
                     /translation="MFSRLGTWSHDHRKLVLGLWVVALVAANIIAGGIGDAYRQDFSL
                     NGFESTEGFKLVESQFSDGSGSPQSGQIVFSADQGVSDPEVKAAMEEMFEKVGAIEDV
                     TSVQSPYASGGEFQISNRGPLAGKIAYATVNLPEDIDFTRASEIGKEIRKLTPEVEGL
                     RIELGGFLFAEFEQPSAELFGLAFAIIILIIAFGSVIAMGLPVAVGLFGIGMGGALVI
                     LASHFLEVPDFAPFIGVMIGLGVGIDYALLIVTRHREQLHSGYDVRQSVAISMDTAGR
                     SVLFAGLTVVVSLLGMLLMGIGFIGGLGITASLTVLATVIASLTLLPALLGFAGTKIE
                     QTKWRGLLASGFVALALAGAGLKVPAVVGLGFLLALLTIVLGFFVPALKREVPRRPAK
                     PRQQTVAYRWSRVIQHRPWTATIAAGGLLLVLAIPVFQLRLGFSDESNFADDTTTKQA
                     YDLVVDGFGKGSTGPIYLVARVDDAATQMGKFAEVTEAVQADRDVESVLGPQPNDPEN
                     PTAVRWFVTPRGGPQDQSTTQLVERLRSDVLPPSIEAVGTDIFVTGLVAANVDMSDYM
                     AERMPIFFGAVLTLSFVLLMIVFRSLLVPLKAVIMNLLSIAAAYGVIIALFQWGWLSD
                     ITGVQPGPIETWVPMMLFAVVFGLSMDYEVFLLSRVREEWKRTGDSRTSVADGLAATA
                     KVITAAAAIMVVVFGSFMLEFERTMKMMGIGLATAIFLDATIVRLVLVPGTMELLGDK
                     NWWLPKWLDRILPNLDVEGHAPPPSEAPVIETEERDREPVPTAGE"
     gene            complement(2629..>3234)
                     /locus_tag="VFX21_03085"
     CDS             complement(2629..>3234)
                     /locus_tag="VFX21_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEX5094964.1"
                     /translation="ITKPVNSAEISCYDPAQVSQPGDGYLHLRAVKRSCTANNGITYQ
                     YASGLVESAHDFTFTYGRMEARMWLPGSAAGGHNWPAFWADGLGKWPSTGELDVMEIL
                     SGGQPCWHFHSLDANGQHQGPGSCARMANPSGWHTFAAEWRPGVVDFFYDGVKVGRLS
                     QWITSSPMFLILNLGISTEHGGDLVTPSETLVDYVRVTSLT"
BASE COUNT          535 a         1053 c         1043 g          603 t
ORIGIN      
        1 gacccgatga cgacgtcgac gaggtcatcg agaccgtcgc gcaacagctc gacgtgctcg
       61 gcgttccgca cgaggagcgc gtgcgcccac cgcgcggccg cggctgacga ctcttcgcga
      121 cgagttgctg agagcgatgc cggatccctg ttccgatgaa tttctgacgc cgcgacagtc
      181 ggacctgcaa acaaggacag gtttcggcgc ggctgctgcg tagcatcggc gcccactctg
      241 tctcggcgaa gggagaccac ctcgtgttca gccgcctcgg cacctggtcg cacgaccacc
      301 gaaaactcgt gctcgggctc tgggtcgtcg cgctcgtcgc cgcgaacatc atcgcgggcg
      361 gcatcggcga cgcgtaccgc caggacttct cactcaacgg cttcgaatcg accgaaggct
      421 tcaagctcgt ggagtcgcag ttcagcgacg gcagcggctc accccagtcg ggacagatcg
      481 tcttcagcgc ggatcaaggg gtcagcgacc ccgaggtcaa ggccgcgatg gaagaaatgt
      541 tcgagaaggt cggcgcgatc gaagacgtca cctcggtaca gagtccctac gcgtccggtg
      601 gcgaattcca gatctcgaac cgaggtccgt tggccgggaa gatcgcctac gcgaccgtga
      661 acctgcccga agacatcgac ttcacgcgcg cgtcggagat cggcaaggag atccgcaagc
      721 tcactcccga agtcgaaggc cttcgcatcg agttgggtgg gttcctcttc gcggaattcg
      781 aacaaccgtc ggctgagctc ttcggactcg cgtttgccat catcatcttg atcatcgcgt
      841 tcggttccgt gatcgccatg ggcttgccgg tcgcagttgg cctgttcggc attggaatgg
      901 gcggcgcgct cgtcatcctc gccagccatt tcctcgaggt tcccgacttc gcgccgttca
      961 tcggcgtaat gatcggcctc ggcgtcggca tcgactacgc gttgttgatc gtgacgcgcc
     1021 atcgcgagca attgcactca ggctacgacg tccgccagtc ggtcgcgatt tcaatggaca
     1081 ctgccggccg ctcggtactg ttcgccgggc tcaccgtggt ggtctcgctg ctcggcatgc
     1141 tgctgatggg catcggtttc atcggcggcc tcgggatcac cgcgtcgctc acggtgctcg
     1201 caacggtgat cgcgtcgctc acactgctgc cggcgctgct cggcttcgca gggacgaaga
     1261 tcgagcagac gaagtggcgc ggtttgctcg cttccgggtt cgtcgcgctt gcgctcgccg
     1321 gtgccggtct gaaggttccg gccgtcgtcg ggctcgggtt cctcctggcg ttgctcacca
     1381 tcgtgctggg tttcttcgtt ccggccctca agcgggaggt gccgcggcgg ccggcgaagc
     1441 cgcgccagca aaccgtggct taccggtgga gccgcgtcat ccaacaccgg ccgtggaccg
     1501 cgacgatcgc ggccggcggc ctgctcctcg tgctggcgat tcccgtgttc cagctgcggc
     1561 tcgggttctc cgacgagagc aacttcgccg acgacacaac aaccaagcag gcatacgacc
     1621 tcgtcgtcga cgggttcggc aaagggtcga ccggtccgat ctatctcgtc gcgcgcgtcg
     1681 acgacgcggc gacgcagatg ggcaagttcg ccgaggtcac cgaggcggtc caggccgatc
     1741 gcgatgtcga gtccgtgctc ggcccgcagc cgaacgaccc cgagaacccg acggcggtgc
     1801 ggtggttcgt gacaccgcgc ggcggaccgc aggaccaatc gacgacacaa ctcgtcgagc
     1861 ggttgcgctc cgacgtgttg ccgccgtcga tcgaggccgt cggtaccgac attttcgtca
     1921 ccggtttggt cgccgccaac gtcgacatgt ccgactacat ggccgagcgc atgccgatct
     1981 tcttcggcgc cgtgctcacg ctgtcgttcg tgttgctcat gatcgtgttc cggtcattgc
     2041 tcgtgccgct caaggcggtg atcatgaacc tgttgtcgat cgcggcggcg tacggcgtga
     2101 tcatcgcgtt gttccagtgg ggctggctca gcgacatcac cggcgtgcaa ccgggcccga
     2161 tcgaaacttg ggtgccgatg atgctgttcg cggtcgtctt cggtctttcg atggactacg
     2221 aggtgttctt gctctctcgg gtgcgagagg aatggaagcg caccggcgac agccgcacct
     2281 cggtggccga cggactcgca gcaaccgcga aggtcatcac cgccgcggcc gcgatcatgg
     2341 tcgtcgtctt cggcagcttc atgctcgagt tcgagcgcac catgaagatg atgggcatcg
     2401 ggctcgcgac cgcgatcttc ctcgacgcca ccatcgtccg tctggtgttg gtgcccggaa
     2461 cgatggaact gctcggcgac aagaactggt ggctgccgaa gtggctcgac cggatcttgc
     2521 cgaacctcga tgtcgagggc cacgccccgc cgccatccga ggcgccggtc atcgaaaccg
     2581 aagagcgcga ccgcgaaccg gtaccgaccg ccggcgagta gcggaggatc aggtgagtga
     2641 agtcacccgc acgtagtcga cgagcgtctc ggacggcgtg acgagatccc cgccgtgctc
     2701 ggtggaaatg ccgaggttga ggatgaggaa catcggcgac gacgtgatcc attgcgacag
     2761 ccgtcctacc ttcacaccgt catagaagaa gtcgacgaca cccggccgcc actccgccgc
     2821 gaacgtgtgc cagccggagg ggttcgccat tcgcgcgcat gaccccggtc cttgatgctg
     2881 accgttcgcg tcgagcgagt ggaagtgcca acacggctgc ccgcccgaaa ggatctccat
     2941 gacgtcgagc tcgcccgtgc tcggccattt tccgagaccg tccgcccaga acgcgggcca
     3001 gttgtgacca ccggcggccg agcccgggag ccacatgcgc gcttccatgc gtccgtaggt
     3061 gaacgtgaag tcgtgcgccg actccaccag tcccgatgcg tactgatacg tgatgccgtt
     3121 gttcgcagtg cacgagcgct tcaccgcgcg caggtgcaga tacccgtcgc ccggttgcga
     3181 cacttgcgcc gggtcgtagc acgaaatttc ggctgagttc acaggcttcg tgat
//
LOCUS       DASUGA010000161         7277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2020976, whole genome shotgun sequence.
ACCESSION   DASUGA010000161 DASUGA010000000
VERSION     DASUGA010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7277
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2020976"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VFX21_03090"
     CDS             complement(<1..879)
                     /locus_tag="VFX21_03090"
                     /inference="COORDINATES: protein motif:HMM:NF041045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5094965.1"
                     /translation="MAPETITRTRDFRHEALFYEGRAEFVRATVQFVRDGLAAGEAVM
                     VATLAEKIAAVKSELGAEAAPVTFADMQVIGRNPARIIEAWHEFITQAAAAGMRSRGI
                     GEPAYAERSAAELRECAHHESLLNDALASTECWLLCPYDTSTLDITVLDGACANHPVI
                     VRGEQRTVSTTYRAPTVQMFAEPLPAPPEDANRFMFDLPRLHTVREIVVREARRAGFS
                     EKRAYELAVAAGEISTNSIQHGGASGTLAVWRDDGHVWVEIRDAGHIHDLLVGRLRPS
                     RAAGGGYGVWLANCLCD"
     gene            1023..3218
                     /locus_tag="VFX21_03095"
     CDS             1023..3218
                     /locus_tag="VFX21_03095"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5094966.1"
                     /translation="MTRTVLGHTVARGEDHALLDGSARYADDLALAPGTCWAVFVRSQ
                     DAHAALRAVDTRDALTQPGVLGIFTDADLALPPATAMSRDRAHDRPRLARDRVRFVGE
                     PIAVVVAQSRAQAHDAAELVIVDTEPLPVVVDPLAALPDDAPLAHGDGWPDAEVVVRA
                     RFVNHRIAPVPMEPNACVAAPGRDGGLEVYASTQSVFGVRGEIAGALGLDADVVVVRA
                     AAVGGGFGAKGGTYNEQIVVAALAQRLGRPVAWTETRRENLLNMTHGRGQIQDVDVGV
                     RRDGTIVGIRVRAVADVGAYRMRGPFIPMVTRFMASGTYRFPHIEFHPVIVETNTTPT
                     GPYRGAGRPEAAAMCERIVELVADELELDAIDVRRRNFVPPDAFPYTTPTGATYDTGE
                     YERALDAALAQSHYAQWRGQQQDRRMLGDRFQLGIGIGCYVEVSGRGGEYGSVTIERD
                     GSATVVTGSVPNGQGHETVWAQIASAVLGVPFEQVRVVHSDTTRVDHGTGTFGSRSLQ
                     LAGSAVHDAAVDVREQARRRVAEMLEASVDDIVVFDDGRLGVAGVPASALTWADIAAA
                     PGDAMHAALDFDSDGSFPFGCHVAVVEVDTETGDTRLVQLTAVDDCGTVVNPMIAEGQ
                     VHGGIAQGIGQALFERVVYDDDGNPLTASLVDYAMPSAAELPSFSVHHTVTPTPKNPL
                     GAKGIGESGTTGSTAAVWNAVVDALKPYGVRHLDMPFTPERVWRAIHGA"
     gene            complement(3244..3618)
                     /locus_tag="VFX21_03100"
     CDS             complement(3244..3618)
                     /locus_tag="VFX21_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094967.1"
                     /translation="MPYRFPDDPHDPAWWDVLRRCAELVARIPELPAVVPDEFVLVGR
                     WDRRGRPNIWEYKHSSTRRLLQLDENLVAYRYVTPRGSPLGPGRYVRRVGGLFEAVYH
                     LELWRQPPQRPQSGAVAVDSGH"
     gene            3712..5481
                     /locus_tag="VFX21_03105"
     CDS             3712..5481
                     /locus_tag="VFX21_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5094968.1"
                     /translation="MTHDAEAVDRLPFGSPEKSARYATDRYLGATGLNWWRCDPTLQF
                     LMRSYLDAGELAWAEPHLDRMGALMGGPVSARAELTDKNPPRLERYDRWGHDVSEVVL
                     PQSFLDSRRDILADSFTTPAVRADASKHGVRNLTILGAAHSYMLDQAEIGMACALGTG
                     GDMVVNLVRQFAPPDVREHVLAKFESGEWAGETIQSLTERTGGSDLAELETTATPDGD
                     AWRINGFKWFASNANGQAWVVLAKPEGAPDNTRGIATFLVLRERRDGTRNGIRIRRLK
                     DKLGTKAVASAEVEFADAEAFLLSGQSGGETGAGDGRGLARMMELTNGARLGIAMMGL
                     GCARRALVEAMCYARARDAFGKPLSEQPLMQRKLAELIVDVEAAQALVFDGFDPKHRL
                     RIGAPLIKLRASRLGITAASDAIEVHGGNGYVENWPVARILRDAQVNPLWEGPDNILC
                     LDVRRGIERDQADVAFLDRLRSAVAAGPPDETTALVGERVDQLAAAIDHWKTLDRTTA
                     EARLFPLAELMVDVYAGALLVAQAGFERDQLGTDRKSLIARLYARRHLADLGPWRGVD
                     LPPEDLERFKDLWDGALVDNGVR"
     gene            5635..6801
                     /locus_tag="VFX21_03110"
     CDS             5635..6801
                     /locus_tag="VFX21_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEX5094969.1"
                     /translation="MAGDRTLRGAAAIVGVADAASPTGELDRYGRALEADMIRAALDD
                     AGLTIDDVDGVCSGGFGAPGLAEYLGVNARFLDGTMVGGSSYELHVEHAAAAIAAGVC
                     DVVVSVYASTPRGDRTRGGGGRFGPPPGPNPMVDWEMPFGLRMPMGAYALAASRHMAV
                     YGTTSEQLAQIAVDTRRWASMNPNARYRDPITIDDVLASPLQASPLHLLDCCLVTDGA
                     GAFVMTSAERARDLPKPPVYVLGAATGSDHSMITAMPDLATTAGTISGPTAFKMAGIT
                     TDDVDLLMGYDSFTITALLHLEDLGFCAKGEGGAFVEDGKCGPGGSLPMNTNGGGLSY
                     THPGQYGMFLIVEAVKQLRGECGARQLDDPHIAVAHGSGGVLSTMGTVVLGREL"
     gene            6798..7196
                     /locus_tag="VFX21_03115"
     CDS             6798..7196
                     /locus_tag="VFX21_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX5094970.1"
                     /translation="MKLLEPPISEEAVPYWDATREQKLVLPWCTACNAPFWYPRPTCP
                     RCLGDNVEWKPASGTGEVYAVSVQYKPGPMREEADGPYAVALVDLAEGVRMMTNVVGC
                     APEVVTVGMAVKVHWHALSDGRHLPFFAPE"
BASE COUNT         1115 a         2479 c         2592 g         1091 t
ORIGIN      
        1 gtcgcagaga cagttcgcga gccacacgcc gtacccaccg cccgcggcgc gggaggggcg
       61 gagccggccg acgaggaggt cgtggatgtg ccccgcgtcg cggatctcga cccagacgtg
      121 tccgtcgtcg cgccacaccg cgagtgttcc ggacgcgcca ccgtgctgga tgctgttggt
      181 ggagatctcg ccggcggcga ccgcgagctc gtaggcgcgc ttctccgaga atccggcgcg
      241 gcgtgcttcg cggacgacga tctcgcgcac cgtgtgcaac cgaggaagat cgaacatgaa
      301 gcggttggcg tcctcgggcg gcgccggcaa cggctcggcg aacatttgca ccgtcggcgc
      361 gcgataggtg gtgctcaccg ttcgttgctc accgcgcacg atgacggggt gattggcgca
      421 cgcgccgtcg aggacagtga tgtcgagtgt gctcgtgtcg tagggacaca agagccagca
      481 ctccgtggag gcgagtgcgt cgttcagcag cgactcgtga tgtgcgcact cgcgcaactc
      541 ggccgcgctg cgttcggcgt acgccggctc accgatgcct cgcgaccgca tgcccgcggc
      601 ggccgcctgc gtgatgaact cgtgccacgc ctcgatgatg cgcgccgggt tgcggccgat
      661 gacctgcatg tcggcgaagg tgacgggcgc ggcctcggcg ccgagctcgc tcttcacggc
      721 ggcgatcttc tcggccaacg tggcgaccat cactgcctcg ccggcggcca ggccatcgcg
      781 gacgaactgc acggtggcgc gcacgaactc ggcccggccc tcgtagaaga gcgcctcgtg
      841 gcgaaagtcg cgcgttcgtg tgatcgtttc cggggccatg ggtcgtactg ggagaggtgc
      901 cgccgcctgg tacggccgga tttccggtcg ccgtaccccg atcgtgcccg atttgcacct
      961 cgtgacgccg aaagaacgac cggggatccg gacgaatccc gggacaaggc agactcggac
     1021 atgtgacgcg cacggtcctc ggccacaccg tcgctcgggg cgaggatcac gccctcctcg
     1081 acggttccgc acgctacgcc gacgatctcg ccctcgcgcc ggggacgtgc tgggctgtct
     1141 tcgtgcggtc gcaggacgcg cacgcggcgc tgcgggccgt cgacacccgt gacgcactga
     1201 cgcagccggg tgtgctcggc atcttcaccg acgccgatct cgcgctcccg ccggcgactg
     1261 cgatgagccg cgaccgggcc catgaccgtc cgcgcctggc tcgcgaccgc gttcggttcg
     1321 tgggcgaacc cattgcggtt gtcgtcgcgc agtcccgggc ccaggcgcac gacgcggccg
     1381 agctggtgat cgtcgacacc gaaccactgc cggtcgtcgt cgacccgctc gcggcgctcc
     1441 ccgacgacgc gccgctggcc cacggcgacg gttggccgga cgcggaggtc gtcgtccgcg
     1501 cccgattcgt caaccatcgc atcgcgccgg ttccgatgga gccgaacgcg tgtgtcgccg
     1561 cacccggtcg cgacggaggc ctcgaggtct acgcgagcac gcagtcggtc ttcggtgttc
     1621 ggggcgagat cgcgggtgcc ctcggtctcg acgccgacgt cgtcgtcgta cgggccgcgg
     1681 ccgtgggtgg gggattcggc gccaagggcg gtacgtacaa cgagcagatc gtggtcgccg
     1741 cgctcgcgca gcggctcggc cgaccggtcg cgtggacgga gacgcggcgc gagaacctgt
     1801 tgaacatgac gcacggccgc ggacagatcc aagacgtcga cgtcggggtc cggcgcgacg
     1861 gcacgatcgt gggcatccgt gtgcgcgcgg tcgcggacgt cggtgcttac cgcatgcgcg
     1921 gcccgttcat cccgatggtc acgcgtttca tggcatcggg gacctatcgc ttcccccaca
     1981 tcgagttcca tccggtgatc gtcgagacga acacgacgcc gaccggcccc taccgcggcg
     2041 ccgggcggcc ggaagccgcc gcgatgtgcg aacgcatcgt cgagctcgtc gccgacgaac
     2101 tggagctcga cgcgatcgac gtgcgacgcc gcaacttcgt gccgcccgac gcgttcccct
     2161 acacgacccc gaccggcgcg acctacgaca ccggcgagta cgagcgtgcg ctcgacgccg
     2221 cgctggcgca gagtcactat gcccagtggc gcggccaaca gcaggaccgc cggatgctcg
     2281 gcgaccggtt ccagcttggt atcggcatcg ggtgctacgt cgaagtgagc ggccgcggtg
     2341 gcgagtacgg atcggtcacg atcgagcgcg acggcagtgc caccgtggtg acgggcagcg
     2401 tgccgaacgg gcagggccac gagaccgtct gggcgcagat cgcgtcggcg gtgctcggtg
     2461 tccccttcga gcaggtgcgc gtcgtgcact cggacacgac gcgcgtcgat cacggcaccg
     2521 gcacattcgg ttcccggtcg ttgcagctgg cgggaagcgc ggtgcacgac gccgccgtcg
     2581 acgtgcgcga acaggcgcgg cggcgggtcg ccgagatgct cgaagcatcg gtcgacgaca
     2641 tcgtcgtctt cgacgacgga cgcctcggcg tcgcgggcgt cccggcgtcc gcgctgacgt
     2701 gggccgacat cgcggcggcg cccggcgacg cgatgcacgc cgcgctcgac ttcgactccg
     2761 acggatcgtt cccgttcggc tgccacgtcg cggtcgtgga ggtcgacacg gaaacgggcg
     2821 acacgcggct ggtgcagctg acggccgtcg acgactgcgg caccgtcgtg aaccccatga
     2881 tcgccgaggg ccaggtgcac ggcgggatcg cgcagggcat cgggcaggcg ttgttcgaac
     2941 gagtcgtgta cgacgacgac gggaacccgc tcaccgcgag cctcgtcgac tacgcgatgc
     3001 cgagcgccgc ggaactgccg tcgttctcgg tgcatcacac cgtgacgccg acaccgaaga
     3061 acccgttggg tgcgaagggc atcggtgagt cggggacgac cggctcgacc gccgcggtgt
     3121 ggaacgcggt cgtcgacgcg ctgaagccgt acggcgttcg ccacctcgac atgcccttca
     3181 cacccgaacg ggtgtggcgg gcgatccacg gtgcctgaac cggcagtcgt cgacttcctc
     3241 gcctcagtgc cctgagtcga ccgcgacggc cccgctttgc ggtcgttgtg gcggctgccg
     3301 ccacagctcg aggtggtaca cggcttcgaa cagtccgccg acgcgccgca cgtaacggcc
     3361 cggccccagt gggctgccgc gcggcgtgac gtagcggtac gcgacgaggt tctcgtcgag
     3421 ctgcagcagc cggcgggtgg acgagtgctt gtactcccag atgttcgggc ggccgcgccg
     3481 gtcccagcgg ccgacgagca cgaactcatc cgggaccacc gccgggagct cggggatgcg
     3541 agccacgagc tccgcgcaac ggcgcaggac gtcccaccac gccggatcgt gcgggtcgtc
     3601 cgggaatcgg tacggcattg cttccccttg gctcggcctt gtacggcatg gggaagttcg
     3661 aacagcatgc ccgaggggtg tgacaagacc gcgcggtgga aggatggcgt catgacgcac
     3721 gacgcggaag ccgtcgatcg tctgccgttc gggtcgcccg agaagagcgc ccgctacgcg
     3781 accgaccggt acctcggcgc aacgggactc aactggtggc gttgcgaccc gacgctccag
     3841 ttcttgatgc ggtcgtacct cgacgccggc gaactggcgt gggccgaacc gcatctcgat
     3901 cgtatgggcg cgttgatggg cggcccggtg tcggcgcgcg ccgagctgac cgacaagaac
     3961 ccgccgcggc tcgagcgcta cgaccgctgg ggacacgacg tcagcgaagt cgtgctgccg
     4021 cagtcgttcc tcgactcgcg ccgcgacatc ctcgccgaca gcttcacgac gccggccgtc
     4081 cgtgcagacg cgtcgaagca cggcgtgcgc aacctgacga tcctcggcgc cgcgcacagt
     4141 tacatgctcg accaggccga gatcggcatg gcgtgcgcgc tcggcaccgg cggtgacatg
     4201 gtcgtgaacc tcgtccgcca gttcgcgccg cccgacgtgc gcgagcacgt gctcgcgaag
     4261 ttcgagtcgg gcgaatgggc cggcgagacg atccagtcgc tgaccgagcg caccggcggt
     4321 tccgacctcg ccgagctgga gaccaccgcg acgcccgacg gtgacgcgtg gcgcatcaac
     4381 ggcttcaagt ggttcgcgtc gaacgcgaac ggccaagcgt gggtggtgct cgccaaaccc
     4441 gaaggcgcgc ccgacaacac gcgcggcatc gcgacgttcc tcgtgctacg cgagcggcgt
     4501 gacgggacgc gcaacggcat tcgtatccgc cggttgaagg acaagctcgg tacgaaagcg
     4561 gtcgcgtccg cggaagtgga gttcgccgac gccgaagcgt tcctcctgtc gggacagagc
     4621 ggtggcgaga ccggcgccgg cgacggtcgc gggctggcgc gcatgatgga gctcacgaac
     4681 ggcgcgcggc tcggcattgc gatgatgggc ctcggctgtg cgcggcgcgc gctcgtcgaa
     4741 gcgatgtgtt acgcgcgggc gcgcgatgcg ttcggcaagc cgttgtcgga gcagccgttg
     4801 atgcagcgca agctggcaga gctgatcgtc gacgtcgaag ccgcgcaggc gctcgtgttc
     4861 gacggcttcg atccgaaaca ccggctgcgt atcggcgcac cgttgatcaa actgcgcgcg
     4921 tcgcggctcg gcatcaccgc cgcgtcggac gcgatcgaag tgcacggcgg caacggctac
     4981 gtcgagaact ggccggtggc gcgcatcctg cgcgacgcgc aggtgaaccc gctgtgggaa
     5041 gggcccgaca acatcctttg tctcgacgtg cggcgcggca tcgagcgcga ccaagcagac
     5101 gtggcgttcc tcgatcgcct gcgctcggcc gtcgcggccg ggccgccgga tgagacgacc
     5161 gcactcgtcg gcgaacgcgt cgaccagctc gccgccgcga tcgaccactg gaagacgctc
     5221 gaccgcacca cggccgaagc gcgcctgttc ccgttggccg agctcatggt cgacgtctac
     5281 gcgggtgcgt tgctcgtcgc gcaggccggc ttcgaacgcg accagctcgg cacggatcgc
     5341 aagtcgttga tcgcccggct gtacgcacgc cgccacctgg ccgacctcgg cccatggcgc
     5401 ggcgtcgacc tcccgcccga ggatctcgaa cgcttcaaag acctctggga cggcgcgctg
     5461 gtagacaacg gcgtgcgctg actgccgttt tcgtcatcag attgcaagag ccgccagcag
     5521 gtggccggtc tcgcattctg atggcaaatc gcgcagctac tcgcggcggc gctcggactc
     5581 gggtcgcgcg cacgaaattg acggctgcgc ggacggcgca gcacgcttcc gccgatggca
     5641 ggcgatcgga cattgcgcgg cgcggccgcg atcgtcggtg tcgccgacgc ggcgtcaccg
     5701 accggtgagc tcgaccgcta cggccgcgcc ctcgaagccg acatgatccg cgccgcgctc
     5761 gacgatgccg gactcacgat cgacgacgtc gacggcgtgt gctccggcgg attcggcgca
     5821 cccggcctcg ccgagtacct cggtgtcaac gcccgcttcc tcgacggcac gatggtcggc
     5881 ggctcgagct acgagctcca cgtcgagcac gcggcggccg cgatcgcggc gggcgtgtgc
     5941 gacgtcgtcg tgagcgtgta cgcgtcgaca ccgcgcggtg accgcacgcg cggcggcggt
     6001 gggcgcttcg ggccgccgcc cgggcccaac ccgatggtcg actgggagat gccgttcggg
     6061 ttgcgcatgc cgatgggcgc gtacgcgctc gcggccagcc ggcacatggc ggtctacggc
     6121 acgacgagcg agcagctcgc ccagattgcc gtcgacacgc gtcggtgggc gtcgatgaac
     6181 ccgaacgcgc gctatcgcga tccgatcacg atcgacgacg tgctcgcctc gccgttgcag
     6241 gcgtctccgt tgcacttgct cgactgctgc ctcgtgaccg acggtgcggg cgcgttcgtg
     6301 atgacgtccg ccgaacgggc gcgcgacctc ccgaagccgc cggtctacgt gctcggcgcg
     6361 gcaaccggca gcgatcactc gatgatcacc gcgatgcccg acctcgcgac gaccgcgggc
     6421 acgatctccg gcccaacggc gttcaagatg gccggtatca ccaccgacga cgtcgacctc
     6481 ctcatgggct acgactcgtt cacgatcacc gcgctgttgc atctcgaaga cctcgggttc
     6541 tgcgcgaagg gtgaaggcgg cgccttcgtc gaagacggca agtgcgggcc cggtgggtcg
     6601 ttgccgatga acaccaacgg cggcgggctg tcgtacacgc acccgggtca gtacggcatg
     6661 ttcctcatcg tcgaagcggt gaagcagctg cgcggcgaat gcggtgcgcg ccaactcgac
     6721 gacccgcaca tcgcggtcgc gcacggctcg ggtggtgtgc tctcgacgat gggcaccgtg
     6781 gtattgggaa gagaactatg aagctgctcg aaccgccgat ctccgaagaa gcagtcccgt
     6841 actgggacgc gacgcgcgaa cagaagttgg tgctgccgtg gtgcacggcg tgcaacgcgc
     6901 cgttttggta tccgcgaccg acgtgcccgc ggtgcctcgg tgacaacgtc gagtggaagc
     6961 cggcgtcggg cacgggtgag gtgtacgcgg tgagcgtgca gtacaaaccg ggcccgatgc
     7021 gcgaggaagc cgacggcccg tacgcagttg cgctcgtcga tctcgcggaa ggcgtccgca
     7081 tgatgacgaa cgtcgtgggc tgcgcgccgg aggtagtgac ggtcggcatg gcagtgaagg
     7141 tgcactggca tgcactctcc gacgggcggc atctgccttt ctttgcaccc gaataggggg
     7201 aaagcgcgca tatggagatc gatctcggcc ccgcggcgac caagttccgc gacgaggtac
     7261 gcgactggct cgcggcc
//
LOCUS       DASUGA010000162         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_371061, whole genome shotgun sequence.
ACCESSION   DASUGA010000162 DASUGA010000000
VERSION     DASUGA010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371061"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VFX21_03120"
     CDS             <1..355
                     /locus_tag="VFX21_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094971.1"
                     /translation="VVGFGLLDHERVPKRAYGAVRDACRPVLATVDPRTGSVHIVNDG
                     RVEIEDAEIEVSVDGRVRRWRGSVDADAIAYVGRVDLDDAVDVEVVLTHASTGRVANR
                     YPLLVLEAGRRNTVS"
     gene            465..1283
                     /locus_tag="VFX21_03125"
     CDS             465..1283
                     /locus_tag="VFX21_03125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HEX5094972.1"
                     /translation="MTTTETPPTAGTAETPPPAAATPRVRGLGRARTPIGRMYATGIG
                     KKWVMAITGLLLMSFLVVHLLGNIKLYLSKEELNLYGEALRDMPGHLLPRTWLLWGFR
                     IGLIAAFFFHIHAAYGLTRINMKARPEKYQSKRDYIAADFASRTMRWTGIIILLYLIF
                     HLADLTWGSANSEFLRGDPYNNLVYSLQRPVVAIAYGAAMIALGFHLYHGSWSLFQSL
                     GINNPRYNRGRRLFAIGFAVVIAGGNLIFPIAVQAGWVGLECPHTEPTTISCPE"
     gene            1292..>2395
                     /gene="sdhA"
                     /locus_tag="VFX21_03130"
     CDS             1292..>2395
                     /gene="sdhA"
                     /locus_tag="VFX21_03130"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase (quinone) flavoprotein
                     subunit"
                     /protein_id="HEX5094973.1"
                     /translation="MAFPDSKAPDGPLAQKWDQHKFDVKLVNPANKRRFEIIVVGTGL
                     AGASAAASLSELGYKVKVITFHDSPRRAHSIAAQGGINAAKNYKNDGDSIQRLFYDTI
                     KGGDYRAREANVYRLAQVSVDIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYAR
                     GQTGQQLLLGAYQALMKQVHAGGVTLYPRTEMLDLVVKDGKACGIVCRDLNTGTVFSM
                     SGHAVVLATGGYGNAFYLSTNAKNSNVTAAWRAAKKGAFFANPCYTQIHPTCIPASDE
                     FQSKLTLMSESLRNDGRVWVPKQMDDGRSPDQIPADDRDYFLERRYPAFGNLVPRDVA
                     SRAIKREVDAGRGVGPLKNGVYLDFSESIERLGV"
BASE COUNT          413 a          810 c          760 g          412 t
ORIGIN      
        1 cgtcgtgggg ttcggtctgc tcgaccatga acgggtgccc aagcgtgcgt acggcgcggt
       61 ccgcgacgcc tgccgtccgg tgctggcgac ggtcgacccc cgtaccggca gcgttcacat
      121 cgtgaacgac gggcgcgtcg agatcgagga tgccgagatc gaggtgtccg tggacggccg
      181 cgtccggcgc tggcggggct cggtcgacgc cgacgcaatc gcctacgtcg gccgggtcga
      241 tctcgacgac gcggtcgacg tcgaggtggt gttgacgcac gcgtcgaccg ggcgcgtcgc
      301 gaaccgatat cccctgctcg tcctcgaagc cggccggcga aacaccgtct cctgaacgga
      361 gaacgtgaac cgattcacga ttcgtcaacg tttcgggact gcagcgtaaa gtcggttcac
      421 aaggtcacga accccaggcg attcgtgctc gtttcaggag agcaatgacc accacggaga
      481 caccgccgac cgcggggacg gcggaaacac cgccgccggc agcggcgaca ccgcgcgtcc
      541 gaggtctcgg ccgcgcccgc acaccaatcg gccgcatgta cgcgaccggc atcggcaaga
      601 agtgggtgat ggccatcacc ggcctcctgc tcatgtcgtt cctggtcgtg cacctgctcg
      661 gcaacatcaa gctctatctc tccaaagagg aactcaatct ctacggcgag gcactgcgcg
      721 acatgcccgg ccacttgctg cctcgcacgt ggttgctgtg gggcttccgg atcggcctga
      781 tcgcggcatt cttcttccac atccacgccg cgtacggcct gacgcgtatc aacatgaagg
      841 cgcggccgga gaagtatcag tcgaagcgcg actacatcgc cgccgacttc gcatcgcgca
      901 cgatgcggtg gacgggcatc atcatcctgc tctacctcat cttccacttg gccgacctca
      961 cgtggggcag cgcgaactcg gagttcttgc ggggcgatcc ctacaacaac ctcgtttatt
     1021 cgttgcagcg gccggtcgtc gcgatcgcgt acggggcggc gatgatcgcc ctcggcttcc
     1081 acctgtacca cggcagctgg tcgctctttc agtcgctcgg gatcaacaac ccccggtaca
     1141 accgaggtcg gcggctgttc gccatcggct tcgcggtcgt catcgcgggc ggcaatctca
     1201 tcttcccgat cgcggtgcaa gcgggctggg ttgggctcga atgtccgcac acggaaccga
     1261 ccacgatttc gtgccccgag taggtctgca catggcattc cccgattcga aagcacccga
     1321 cggtcccctc gcccagaagt gggaccagca caagttcgac gtgaagctcg tgaaccccgc
     1381 gaacaagcgg cggttcgaga tcattgtcgt cggcaccggc ctcgccggcg cgtcggccgc
     1441 ggcgtcgttg tcggaactgg gttacaaggt gaaggtcatt accttccacg actctccccg
     1501 tcgcgcgcac tcgatcgccg cgcagggcgg catcaacgcc gccaagaact acaagaacga
     1561 cggcgacagc atccagcggc tcttctacga cacgatcaag ggcggcgact accgcgcccg
     1621 cgaggcgaac gtgtaccgcc tcgcgcaggt gtcggtcgac atcatcgatc agtgcgtcgc
     1681 acagggcgtg cccttcgccc gtgagtacgg cgggctgctc gacaaccggt cgttcggtgg
     1741 cgcgcaggtg tcgcgcacct tctacgcccg cggccagacc ggccagcagt tgctactcgg
     1801 tgcgtaccag gcgttgatga agcaggtgca cgcgggcggc gtcacgctct atccccgcac
     1861 cgaaatgctc gatctcgtcg tcaaggacgg caaggcgtgc ggcatcgtgt gccgcgacct
     1921 gaacaccggc accgtgttct cgatgtccgg tcacgcggtc gtgctcgcga ccggcgggta
     1981 tggcaacgcc ttctacctgt cgaccaacgc gaagaactcg aacgtgacgg ccgcgtggcg
     2041 ggcggccaag aagggcgcgt tcttcgccaa cccctgctac acgcagatcc acccgacctg
     2101 cattccggcc agcgacgagt tccagtcgaa gctcacgttg atgtccgagt cgttgcgcaa
     2161 cgacggacgc gtctgggttc ccaagcagat ggacgacggc cgttcgcccg accagatccc
     2221 ggccgacgac cgcgactact tcctcgaacg gcgctacccg gcgttcggca acttggtgcc
     2281 gcgcgacgtt gcgtcccgcg cgatcaagcg cgaggttgac gcgggccgcg gtgtgggtcc
     2341 gttgaagaac ggcgtgtacc tcgacttctc ggagtcgatc gagcggctcg gtgtc
//
LOCUS       DASUGA010000163         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_594789, whole genome shotgun sequence.
ACCESSION   DASUGA010000163 DASUGA010000000
VERSION     DASUGA010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594789"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VFX21_03135"
     CDS             complement(<1..690)
                     /locus_tag="VFX21_03135"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5094974.1"
                     /translation="MTDSPSTWVAVLFTDVVGSTRLWAEHHDAMASDLAIHDGLLRDA
                     IAAHGGRVFSTAGDAFAAAFPSASQAVDAAIDGQRAMSDTPWSVPGGIQIRTAVHAGE
                     VIERGGDYFGPTLNTGARVLSAGHGGQVLVTAGVATAIPHPSTDLGAHRLRDIETPVR
                     IHQLRAPGLEEEFPPLASLASGQSRLPQHRSSFVGRADEMRNLRQMLHDSRLVTVTGV
                     GGVGKTRIAVEV"
     gene            complement(733..>2083)
                     /locus_tag="VFX21_03140"
     CDS             complement(733..>2083)
                     /locus_tag="VFX21_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5094975.1"
                     /translation="CKHPSVRAPETKYAKSSDGVHIAYQECGDGPLDIVMVPGFVSHV
                     ELIWEIPFARQQFEYLASYARLIRFDKRGTGLSDRHLGIPTLEQRMDDVRAVMDAAGS
                     EHATLYGVSEGGPMCILFAATYPERTDALVLSGSFAKLASGPDQPFGQSPEALEAQKQ
                     LMVEHWGTGAIFSAFAPGSELDDDVLELFARGERNAARPGDLDQVVRMCIEIDVRPVL
                     PTITVPTLIQHVAGDTIVSVEHGRYLADHIPDATYIEYEGRDHMTVDETERLGQLENM
                     REFLTGTRAAPDFDRVLATVLFTDIVSSTERAAQVGDAAWKRVLDQHDEIMQTQIGRF
                     RGRAVKSTGDGFLAVFDGPGRAAQCALAAKDALRKLDIDIRAGVHTGEIEQRGDDVGG
                     IAVHIGARIASKADAGEVLASRVVRDLVVGSSLEFSERGAHELKGVPGSWELYAVAV"
BASE COUNT          309 a          712 c          740 g          322 t
ORIGIN      
        1 cacctcgacc gcgatccgcg tcttgccgac gccacccacc ccggtcacgg tcacgagccg
       61 gctgtcgtgg agcatctgac ggaggttgcg catctcgtcc gcgcgcccga cgaacgacga
      121 ccggtgctgc ggcaaccgcg attggcccga cgcgagggag gcgagcggcg ggaactcctc
      181 ctcgagtccc ggcgcgcgca gttggtggat ccgcacgggt gtctcgatgt cgcgcaagcg
      241 gtgggcgccg aggtcggtgc tcgggtgggg gatcgcggtg gcgacgcccg cggtgacgag
      301 gacctgcccg ccgtggccgg cgctgaggac ccgggcgccg gtgttcaggg tggggccgaa
      361 gtagtcgccg ccgcgctcga tcacctcacc ggcgtgcacc gcggttcgga tctggatccc
      421 acccggaacg gaccatggcg tgtccgacat ggcccgctga ccgtcgatgg cggcgtcgac
      481 cgcctggctc gcggacggga acgcggccgc gaaggcgtcg ccggccgtcg agaagacgcg
      541 accaccgtgc gccgcgatcg cgtcgcggag cagtccgtcg tggatggcca gatcggatgc
      601 catcgcgtcg tggtgctccg cccacagccg tgtggacccg accacatcgg tgaagagcac
      661 cgccacccac gtcgacggtg agtccgtcac gggcgccagt ctgtcaggag cttggccggc
      721 gagtcgttcc gttcagacgg cgacggcgta gagctcccag gaaccgggta cgcccttcaa
      781 ctcgtgcgcg cctcgctcgc tgaactcgag cgacgaaccg accacgaggt cgcgcacgac
      841 ccgcgatgcg agcacttcgc cggcatctgc cttcgatgcg atgcgagcac cgatgtggac
      901 cgcgatgccg ccgacgtcgt cgccgcgctg ttcgatctcg ccggtgtgca cgccggcccg
      961 gatgtcgatg tcgagcttgc gcaacgcgtc cttggccgcg agggcgcatt gtgcagcccg
     1021 gccgggtccg tcgaacacgg cgaggaagcc gtcgcccgtc gacttgaccg cgcgaccgcg
     1081 aaaccgcccg atctgggtct gcatgatctc gtcgtgctgg tcgagcaccc gcttccatgc
     1141 ggcgtcgccc acctgcgcag cgcgttcggt ggacgacacg atgtcggtga acaggacggt
     1201 ggcgagcaca cggtcgaagt ccggagccgc acgggtgccg gtcaagaact cgcgcatgtt
     1261 ctccagttgg ccgagccgct cggtctcgtc gaccgtcatg tgatcgcggc cctcgtactc
     1321 gatgtacgtc gcgtcgggga tgtgatccgc caggtatcga ccgtgctcga ccgacacgat
     1381 cgtgtcgccg gccacgtgtt ggatcagggt cggcacggtg atcgtcggca acaccggccg
     1441 cacgtcgatc tcgatgcaca tccgtacgac ttggtcgaga tcgcccggtc gcgccgcgtt
     1501 gcgctcgcca cgcgcgaaca gctcgagcac gtcgtcgtcg agctcggagc cgggcgcgaa
     1561 cgccgagaag atcgcgccgg tgccccagtg ctcgaccatc agctgcttct gtgcctccag
     1621 cgcctcgggc gactggccga agggctggtc gggcccggac gcgagcttgg cgaacgagcc
     1681 gctgagcacg agggcgtcgg tgcgctcggg gtaggtggcc gcgaacagga tgcacatcgg
     1741 cccgccctcg gacaccccgt acagcgtggc gtgctcggag ccggccgcgt ccatgacggc
     1801 gcggacgtcg tccatgcgct gttccagggt ggggatgccg agatggcggt ccgacagacc
     1861 cgtgccgcgc ttgtcgaagc ggatcaggcg cgcgtacgag gcgaggtact cgaactgttg
     1921 gcgcgcgaag gggatctccc agatcagctc gacgtgcgag acgaagcccg gaaccatgac
     1981 gatgtcgagc gggccatcgc cgcactcctg gtacgcgatg tggacgccgt cgctgctctt
     2041 cgcgtacttc gtctctggcg cccgcacgga cggatgcttg cac
//
LOCUS       DASUGA010000164         3299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1985158, whole genome shotgun sequence.
ACCESSION   DASUGA010000164 DASUGA010000000
VERSION     DASUGA010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1985158"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1781)
                     /locus_tag="VFX21_03145"
     CDS             complement(<1..1781)
                     /locus_tag="VFX21_03145"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5094976.1"
                     /translation="MQTSGVFAREVVLREALVLKVSTEDRAVLETLARYAFARPGFRA
                     ETVVRYGDGGTETATFEILHDEDGPIVRRITDDAGNRICDHEAVDDQSVGEWTEPDPR
                     GNGHVSEDAESAEDEQFDAADLDHVLMQTAFRTPDLLAVFSSGGRELRWCNEAFERAL
                     GPVVSEHPRLVELLDDWSQGHFMVKGLAALIRQSWWRGRLTFEGAGDTRLAVSATVLA
                     HRDPDGEIDSLSLSATPIEEDENAPVDDATALAALVENVSELLAVIETDGRVRFASPG
                     LTHLLQRRNGALVGLDFLGLLHPDDAVRDVAALARVDHDGNAEPVRVRLRAADQSWRH
                     LDAVVTDLRSNPSINGFVLSARDVTDRVESFDRLAAEAFTDPDTGLPNRFRLLDRLTT
                     LLRDSHTSGAAAVLLVDLDGLRHVNDHGLAFGDAFLVEVARRLVDSAGPGSFVARLRS
                     DEFAVVLPDVTDPAVGRRVADALRVALAKPFEHEDVIVRVTASIGVALGRAGEDPDEL
                     LRRADLACIEAKQARGNTTVLWGAEMARRESRRRDVELQLRHALDHAGIQPHYQPIVD
                     LDTGKVVGAEALLRLRDTDGGLLTPGE"
     gene            2028..2639
                     /locus_tag="VFX21_03150"
     CDS             2028..2639
                     /locus_tag="VFX21_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016867483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX5094977.1"
                     /translation="MHSRKRPRLDIGAPAKLAQVEQLTDLELEAALDTIRSSPTDLGT
                     VELLVCRTAVGERDTPLEIELDTELGVVGDNWSTRPNRRTPAGGPDPNGQVTLVNARA
                     LAVVAGDRAAWPQAGDQLYVDFDLSARNLPPGSRVVVGDAVLELTAKPHLGCAKYARA
                     FGRDAVRFVNSPEGKALRLRGANAKVVRSGVVRVGDEIRKSKS"
     gene            2695..>3299
                     /locus_tag="VFX21_03155"
     CDS             2695..>3299
                     /locus_tag="VFX21_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX5094978.1"
                     /translation="MTDELGFETLQIHAGQTPDSATGARAVPIYQTTSYQFRDTSHAA
                     NLFALAEVGNIYTRIMNPTQAVFEARIAALEGAPDTAIGIPGALATSSGQAAETFAIL
                     NLAEPGSHIVSSAALYGGTYNLFHYTLPRLGVDVSFIDDPDDLEQWQKLIQPNTKALY
                     GETIGNPRNDVFDIEGVAEVAHSNGIPLILDNTVPTPWLIRP"
BASE COUNT          547 a         1182 c         1097 g          473 t
ORIGIN      
        1 aactcaccag gcgtgaggag gccgccgtcg gtgtcgcgca ggcgcaacaa cgcttcggca
       61 ccgaccacct tgccggtgtc gagatcgacg atcggttggt agtgcggctg gatgccggcg
      121 tgatcgagcg cgtggcgcaa ctgcagctcg acgtcgcgac gccggctctc ccgccgcgcc
      181 atctcggcgc cccagaggac ggtggtgttg ccgcgcgctt gtttggcttc gatgcacgcg
      241 aggtcggcgc ggcgcaacag ctcgtcgggg tcctcccccg cacgacccag cgcgactccg
      301 atgctggcgg tcacccgcac gatcacatcc tcgtgttcga acggcttggc caacgcgacc
      361 cgcagcgcat cagccacacg tcgccccacg gccggatcgg tgacgtcagg gagcacgacg
      421 gcgaactcat cgctgcgcag acgagcgacg aacgacccgg ggcccgcgct gtcgacgagc
      481 cggcgagcca cctcgaccaa gaacgcatcg ccgaacgcga gaccgtggtc gttcacgtgg
      541 cgcaggccgt cgaggtcgac gagcagcacc gcggccgcgc ccgacgtgtg cgagtcgcgc
      601 agcaaggtgg tgagccggtc gagcagccgg aagcggttgg gcagacccgt gtcgggatcg
      661 gtgaacgctt cggcggcgag ccggtcgaac gactcgacac gatcggtgac atcgcgcgcg
      721 ctgagcacga aaccgttgat cgagggattg gaccgcagat cggtgacgac ggcgtcgagg
      781 tgacgccacg actggtcggc ggcacgtagg cgcacgcgga ccggttcggc gttgccgtcg
      841 tggtcgaccc gcgccagcgc ggcgacgtcg cgcacggcgt cgtcgggatg cagcaggccg
      901 aggaagtcga ggccgaccag cgcgccgttg cgccgctgga gcaggtgcgt gagaccagga
      961 ctggcgaagc gcacccggcc gtcggtctcg atgaccgcca gcagctcgct cacgttctcg
     1021 acgagcgcgg cgagcgcggt ggcgtcgtcg accggcgcgt tctcgtcctc ctcgatgggc
     1081 gtggccgaca acgacagcga atcgatctca ccatcgggat cgcggtgggc caggaccgtc
     1141 gccgacaccg cgaggcgcgt gtcgccggcg ccttcgaagg tcaaccggcc acgccaccag
     1201 ctctggcgga tgagcgcggc cagtcccttg accatgaagt ggccctgcga ccagtcgtcg
     1261 agcagttcga ccaaccgtgg gtgctccgac acgaccggac cgagggcgcg ttcgaacgcc
     1321 tcgttgcacc aacgcagttc acgcccgccg gagctgaaga ccgcaaggag atcgggggtg
     1381 cggaacgccg tctgcatgag cacgtggtcg agatcggccg cgtcgaactg ctcgtcctcc
     1441 gcggattcgg cgtcctccga cacgtgtccg ttgccgcgcg ggtccggttc ggtccactcg
     1501 ccgacggact ggtcgtccac cgcctcgtgg tcgcagatgc ggttccccgc gtcgtcggtg
     1561 atccgccgca cgatggggcc gtcctcgtcg tggaggatct cgaaggtcgc ggtctcggtg
     1621 ccgccgtcgc cgtagcgcac cacggtctcg gcgcggaagc ccggccgagc gaaggcgtag
     1681 cgggcgaggg tctcgagcac ggcccggtcc tcggtgctca ccttgaggac cagcgcctca
     1741 cggaggacca cctcgcgcgc gaacacgcca gaggtctgca tgcgaacgcc cgagccaccc
     1801 ggacccatgc ctctccgctc tagcgatgcc accggctggc cacccttctc ggtcctctct
     1861 gctccgtcga cggattcgcg ctcactatta gggttctgcc cacattcgag cagcgaggta
     1921 ccggcccgcg ccgaaatagc ccgcggtttg ccaattcacc atcctcgatt ggcacctgcc
     1981 tgccgcacga aatgcccttg aagcctacga cacgcgacga ggtcggtgtg cattcccgga
     2041 aaaggcctcg cctcgacatc ggcgcacccg ccaagctcgc ccaggtggag caactgaccg
     2101 atctggagct cgaagccgcc ctcgacacga tccggtcgtc gccgaccgac ctgggcacgg
     2161 tcgaactgct cgtctgccgg accgccgtcg gcgaacggga caccccgctg gagatcgagc
     2221 tcgacaccga gctcggcgtg gtgggcgaca actggagcac gcgcccgaat cgccgcacgc
     2281 ccgccggcgg ccccgatccc aacggtcagg tcaccctggt gaacgcgcgc gccctggccg
     2341 tggtcgccgg tgaccgcgcc gcctggccac aggccggcga ccagttgtac gtcgacttcg
     2401 acctgagcgc gcgcaacctg ccaccgggat cacgcgtggt ggtaggcgac gcagtgctcg
     2461 aactgacggc gaagccgcac ctcgggtgcg cgaagtacgc gcgcgccttc ggtcgggacg
     2521 cggttcggtt cgtcaactcg cccgagggaa aggcgctgcg actccgcggc gccaacgcca
     2581 aggtggtgcg ttcgggcgtg gttcgcgtgg gtgacgagat tcgcaagtcc aaaagttgac
     2641 ctacccggtc gggttttcta gcgtcaaggt tcgaccccat ccgaaggaga cgcggtgact
     2701 gacgaactcg gcttcgaaac cctgcagatc cacgccggcc aaacacccga cagcgcgacc
     2761 ggcgcgcgtg ccgtgcccat ctaccagacg acgtcgtacc agttccgcga cacgtcgcac
     2821 gcggccaacc tcttcgcgct ggccgaggtc ggcaacatct acacgcgcat catgaacccg
     2881 acccaagcgg tgttcgaggc gcgcatcgcc gcgctcgaag gtgcgcccga caccgccatc
     2941 ggcatccccg gcgccctggc gaccagctcg gggcaggcgg ccgagacctt cgccatcctc
     3001 aacctcgccg agccgggcag ccacatcgtg tcgtcggccg cgctctacgg cggcacgtac
     3061 aacctcttcc actacacgct gccgcggctc ggcgtcgacg tgtcgttcat cgacgacccc
     3121 gacgatctcg agcagtggca gaagctcatc cagccgaaca ccaaggcgct gtacggcgag
     3181 accatcggca acccgcgcaa cgacgtgttc gacatcgaag gcgtcgccga ggtcgcgcac
     3241 agcaacggca tcccgctcat cctcgacaac accgtgccca cgccgtggct catccggcc
//
LOCUS       DASUGA010000165         4148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1706932, whole genome shotgun sequence.
ACCESSION   DASUGA010000165 DASUGA010000000
VERSION     DASUGA010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1706932"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VFX21_03160"
     CDS             complement(<1..349)
                     /locus_tag="VFX21_03160"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5094979.1"
                     /translation="MLGRVPARSGTVVDLGCGSGIFSAAMSAAGFDAVGVDISPDMVA
                     LARIAVPSGNFRVGSVHDFEIRAGSVAVVALGEVLNYATDARSGLDALDALSARVYDA
                     LPAGGLFVFDVSTP"
     gene            518..1681
                     /locus_tag="VFX21_03165"
     CDS             518..1681
                     /locus_tag="VFX21_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEX5094980.1"
                     /translation="MEVEIGIGKSGRRAYGFDDIAIVPSRRTRDPEDVDITWEVDAYK
                     LELPMMAAAMDGVVSPSSAIEIGKLGGLACLNLEGLWTRYEDPEAVFEEIANLPPEKA
                     TRRMQELYNEPIKEELIAPRIREIKASGVLACASLTPQRVEQYAKHVIDAELDMLVVQ
                     GTVVSAEHVSHDPERQPLNLKKFIREFDIPVIVGGCASYSTALHLMRTGAVGVLVGVG
                     PGHACTSRGVLGIGVPQATAIADAAGARIRHLDETGVYVHVIADGGMRTGGDIAKAIA
                     CGADAVMIGSPLASAYEAPGRGYHWGMATFHPTLPRGARVFAGRKASMSEILVGPAHE
                     NDGTLNLFGALRTSMATTGYETVKEFQKAEVMVAPALKTEGKTLQRAQGIGMG"
     gene            1681..3216
                     /gene="guaA"
                     /locus_tag="VFX21_03170"
     CDS             1681..3216
                     /gene="guaA"
                     /locus_tag="VFX21_03170"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX5094981.1"
                     /translation="MAENDLVLVVDFGAQYAQLIARRVREAHVYSEIVPRSMPVKEML
                     GRNPKAIILSGGPASVNVDNAPEIDKAIYDAGVPLFGICYGAQLIARDLGGEVAKTGQ
                     GEYGRTELHTEGSSLLLADLPVEQVVWMSHGDSITKAPAGFSITARTEAVPVAGLEDR
                     DRGIYGVQFHPEVMHTEHGQEVLKHFLYDAAGCRPTWTNVSIIEHAVAEIREQVGDAR
                     VICGLSGGVDSAVAAALVHKAIGDQLTCVFVDTGLLRLGEAEQVEETFRDQFSVDLVH
                     VKAQDRFLDALDDVIDPERKRKMIGELFIRVFEEAARDLGDARFLVQGTLYPDIVESG
                     GGDNANIKSHHNVGGLPDDMEFELVEPLRHLFKDEVRAVGEELGLPEEIVWRQPFPGP
                     GLAVRIVGTITRERVEILQHADAIVVEEIRRAGLYREIWQSFAVLPAVKTVGVMGDDR
                     TYAYPIILRAVTSDDAMTADWARIPYDVLERISSRVINEVPGVNRVAYDITSKPPGTI
                     EWE"
     gene            3318..>4148
                     /locus_tag="VFX21_03175"
     CDS             3318..>4148
                     /locus_tag="VFX21_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5094982.1"
                     /translation="MGRAGASMWARADVRGRWKSLVALGILVGITAGFAMAAYAGARR
                     TDTSFERLRERTNAPDAIVFASQSQQFRVEPSVWDDLAKQPEVVKVAPWVLALGAVEG
                     DDEGVIMGSFDNRWIGDIDKGLLVAGRRYDPNAPDEAVVEEYAAKQEGIRVGDTIGYK
                     PFLAKQFENDDEDPGPPAGPQLRIKVVGLVRTIEPHLFAGLVMMSPGVIKHHPDVLYA
                     TNAMVQLKRDSADIGALQSSVNRLIGPGTPILDLHQVQRRVDTTLDVEAAALRLLALA
                     I"
BASE COUNT          741 a         1367 c         1412 g          628 t
ORIGIN      
        1 ccggggtcga gacgtcgaac acgaacagcc cgccggccgg caacgcgtcg tacacccgcg
       61 ccgaaagtgc gtcgagcgcg tcgaggccgc tgcgagcatc ggtcgcgtag ttcagaactt
      121 caccgagcgc gacgacggcg accgagccgg cccggatctc gaagtcgtgg acggaaccca
      181 cgcgaaaatt cccgctcggg acggcgatcc gggccaatgc caccatgtcg ggggagatgt
      241 cgacgccgac ggcgtcgaag ccggcggcgc tcattgccgc cgagaagatg cccgacccgc
      301 aaccgaggtc gacgaccgtt cccgaccggg ccggaacccg cccgagcacc agcgcagccg
      361 cgtcgcgtgc gagctggccg aaccgggcat cgtggatgcg cgcttgatcg ggcccttaaa
      421 acatgtgcgt attgtgcccg ggcacgcttc cgggcggtcg cagcggtacc atccatggtt
      481 tccgaggcgg acggaacaaa caaaacccgg aggggccgtg gaagtcgaga tcgggatcgg
      541 aaagtctggt cgtagggcct acgggttcga cgacatcgcc atcgtgccga gccggcgcac
      601 gcgcgatccc gaggatgtcg acatcacgtg ggaggtcgac gcgtacaagc tcgaacttcc
      661 gatgatggcc gcggcgatgg acggtgtcgt ttcgccgtcg agtgcgatcg agatcggcaa
      721 gctcggcggg ctcgcgtgcc tgaacctcga agggctctgg acgcgctacg aagaccccga
      781 agcggtgttc gaagagatcg cgaaccttcc ccccgagaag gcgacgcgcc ggatgcagga
      841 gctgtacaac gagccgatca aggaagagtt gatcgcgccc cgcattcgtg agatcaaggc
      901 cagcggcgtg ctcgcctgcg catcgctcac gccgcagcgc gtcgagcagt acgccaagca
      961 cgtcatcgac gccgagctcg acatgctggt ggtccaaggc accgtggtct ccgccgagca
     1021 cgtgtcgcac gaccccgagc gccagccgct caacctgaag aagttcattc gcgagttcga
     1081 cattccggtc atcgtcggcg gatgtgcgtc gtactcgacg gcgctgcacc tcatgcgcac
     1141 cggcgcggtc ggcgtcctcg tcggtgttgg tccgggccac gcgtgcacga gccgcggcgt
     1201 gctcggcatc ggcgtgccgc aggcgaccgc catcgccgac gcggccggtg cccgcattcg
     1261 ccacctcgac gagacgggcg tgtacgtcca cgtcatcgcc gacggcggca tgcgcacggg
     1321 cggcgacatc gccaaggcca ttgcgtgcgg cgccgacgca gtgatgatcg gttcgccgct
     1381 cgcctccgcg tacgaagcgc ccggccgcgg ctaccactgg ggcatggcga cgttccaccc
     1441 cacgttgccg cgcggtgctc gcgtgttcgc cggccgcaag gcgagcatgt cggagatcct
     1501 cgtcggaccg gcccacgaaa acgacggcac gctgaacctc ttcggtgcgc tgcgcacgtc
     1561 gatggccacg accggttacg aaacggtcaa ggaattccag aaggccgaag tcatggttgc
     1621 ccccgcgctg aagaccgagg gcaagaccct gcagcgcgcg cagggcatcg gcatgggttg
     1681 atggccgaga acgacctcgt tctcgtcgtc gacttcggcg cccagtacgc acaactcatc
     1741 gcgcggcgtg tgcgtgaggc gcacgtgtac agcgagatcg ttccgcgctc catgccggtc
     1801 aaggagatgc tcggccgcaa cccgaaggcg atcatcctct cgggcggtcc cgcgtcggtg
     1861 aacgtcgaca atgcgccgga gatcgacaaa gcgatctacg acgcgggggt gccgctgttc
     1921 ggcatctgtt acggggcgca actcatcgcg cgcgacctcg gtggcgaggt ggccaagacc
     1981 ggccagggcg agtacggccg caccgaactg cacaccgagg gctcgtcgct gctgctggcc
     2041 gacctcccgg tcgaacaagt cgtctggatg agccacggcg actcgatcac caaggccccg
     2101 gccggcttca gtatcacagc acgcaccgag gcagtaccgg tcgccggcct cgaggaccgc
     2161 gaccgcggca tctacggcgt gcagttccat cccgaggtca tgcacaccga gcacggccaa
     2221 gaggtgttga agcacttcct ctacgacgcg gccggctgcc gtccgacgtg gacgaacgtc
     2281 tcgatcatcg agcacgcggt cgccgagatc cgcgagcagg tgggcgacgc tcgggtcatc
     2341 tgcgggctct cgggcggcgt cgactctgcg gttgcggccg cgctcgtaca caaggcgatc
     2401 ggcgaccagc tcacgtgtgt cttcgtcgac accggcctgt tgcgtctcgg tgaagccgaa
     2461 caggtcgagg agacgttccg cgaccagttc agcgtcgacc tcgtgcacgt caaagcgcag
     2521 gatcggttcc tcgacgccct cgacgacgtg atcgatcccg agcgcaagcg caagatgatc
     2581 ggcgaattgt tcattcgggt gttcgaagag gcggctcgcg acctcggcga tgcgcggttt
     2641 ttggtgcagg gcacgctgta ccccgacatc gtcgaatcgg gtggtggcga caatgccaac
     2701 atcaagagcc accacaacgt cggtggcctg cccgacgaca tggagttcga actggtcgag
     2761 ccgctccgcc acctgttcaa ggacgaagtg cgcgccgtgg gagaagaact cggcttgccc
     2821 gaagagatcg tgtggcgcca gccgttcccg ggtccgggtc tggcggtgcg catcgtcggc
     2881 acgatcacgc gcgaacgggt cgagatcctc cagcacgccg acgcgatcgt cgtggaggag
     2941 atccgccggg ccggcctgta ccgagagatc tggcagagct tcgcggtgct gcccgcggtc
     3001 aagaccgtcg gtgtcatggg cgacgaccgc acgtacgcct atccgatcat tctccgggcc
     3061 gtgacgtccg acgacgcgat gacggcggac tgggcgcgta tcccgtacga cgtgctggag
     3121 cggatctcga gccgggtgat caacgaggtg cccggcgtga accgggtcgc ctacgacatc
     3181 acgagcaagc cgccgggcac catcgaatgg gaataggcga atgggaatag gcgagtggga
     3241 gtagggggct agcaccccca ccattcggcc gcctgccccc tcggcgcggc cccggatccg
     3301 gccgtacgct ccgcgagatg ggacgagcag gcgcgtccat gtgggcgcgg gccgacgtgc
     3361 gggggcgctg gaagtcgctt gtcgcgctcg ggatcctcgt cggtatcacc gccgggtttg
     3421 caatggccgc ctacgccggc gcgcgtcgca ccgatacctc gttcgaacgg ctccgagaac
     3481 gaacgaatgc gcccgacgca atcgtctttg cgtcgcagtc gcagcaattc cgagtcgaac
     3541 cgtcggtgtg ggacgacctc gcgaagcagc ccgaagtcgt gaaggtcgcg ccgtgggtgc
     3601 ttgcactcgg cgcggtcgaa ggcgacgacg agggcgtgat catgggctcg ttcgacaacc
     3661 ggtggatcgg cgatatcgat aagggtctgc tcgtcgcggg ccgtcgctac gacccgaacg
     3721 cgcccgacga agccgtcgtc gaggaatacg cggcgaagca ggaagggatc cgggtcggcg
     3781 acaccattgg ctacaagccg ttcctcgcga agcagttcga gaacgacgac gaagaccccg
     3841 gtccgccggc cggaccgcaa ctgcggatca aggtggtggg cctcgttcgc acgatcgaac
     3901 cgcacctgtt tgccggtttg gtgatgatgt cgcccggcgt gatcaagcat caccccgacg
     3961 tgctctacgc gaccaacgcg atggtgcagc tcaagcgcga cagcgcagac atcggagcat
     4021 tgcagagcag cgtgaaccgc ctcatcgggc ccggcacgcc catcctcgac ctccatcagg
     4081 tgcaacggcg cgtcgacacg acgctcgacg tcgaggcggc cgcgctgcga ctcctcgcgc
     4141 tggcgatt
//
LOCUS       DASUGA010000166         2280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_91584, whole genome shotgun sequence.
ACCESSION   DASUGA010000166 DASUGA010000000
VERSION     DASUGA010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91584"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /locus_tag="VFX21_03180"
     CDS             <1..1106
                     /locus_tag="VFX21_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HEX5094983.1"
                     /translation="ATLPEFAPAANEASAPVRWIFYTSGTTADPKGARHADATVRPSA
                     DAMFEGLHITDDDVFGLVFPYTHIGGQTWTFTALMTGCRLVFNEAFVPAVTIPILRDA
                     DITLAGAGTPFHMAYLEAQRQLPKGEKLFPRVRAFPGGASPKPPQLHYDVVAEMGGVG
                     IVSGYGLTEFPIATMAHVTDSDEELAHTEGRPGPGVTLKLVTLDGRVAGVNEEGEIRT
                     KGRQMFRGYMDSSLDDAAFDEDGWFRTGDLGKVNDHGNVIITGRLKDVIIRKGENISA
                     KEVEDLLFTHPKIADAAVIGLPDPKLGERCVAVIAPADPGDPPTLPELFEFCKESGLM
                     VQKIPEQLEIVDVLPRNPSGKVLKHELRSTYSS"
     gene            1149..>2280
                     /locus_tag="VFX21_03185"
     CDS             1149..>2280
                     /locus_tag="VFX21_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5094984.1"
                     /translation="MRVRRVVAMMIVVVVIAALLLEGIAGAQTNAAPKGTLRWRPCGG
                     SFECSTMRVPLDYANPTGAQVDIAVIRLPAQDDSRRIGSLVVNPGGPGGSGVDFVASQ
                     ADSLPAELRDRFDIVGFDPRGTGQSDPVNCKADMNTYYRLDFSPDDDAERAALVSGIE
                     AFVDACSRNEGSRLPFLSSESTVRDIDELRAALGDNKLTFLGYSYGSYLGAKYAATYP
                     DHVRALVLDGAVDPSLDSKQQQVEQAAGFEGVLNGFLKWCAKNSSCDFHRDGKPAPAL
                     DRLLARVDREGVAVPDTRPARTLSPIEFDLGIASALYEGRGGFSFLASALDAADRGDG
                     RTMADLADSYTERHRDGSYGNIEQAFLATGCADGPPVGTVNDV"
BASE COUNT          362 a          791 c          782 g          345 t
ORIGIN      
        1 ccgcgacgtt gcccgagttc gcccccgcag caaacgaggc ttcggctccc gtgcggtgga
       61 tcttctacac gtcgggcacc accgcggacc cgaagggcgc gcgccacgcc gatgccacgg
      121 tgcggccgtc ggccgacgcg atgttcgagg gcctccacat caccgacgac gacgtcttcg
      181 gcctcgtctt cccgtacacc cacatcggcg gccagacgtg gacgttcacc gcgttgatga
      241 ccggctgccg actcgtgttc aacgaagcgt tcgtcccggc ggtgacgatc ccgatcctgc
      301 gcgacgccga catcacgctc gccggcgcgg gcacgccgtt ccacatggcg tatctcgagg
      361 cgcagcggca gctgccgaag ggcgagaagc tgttcccgcg cgtgcgcgcg ttcccgggcg
      421 gcgcgtcacc caagccgccg cagctgcact acgacgtcgt ggccgagatg ggcggggtcg
      481 gcatcgtgtc gggctacggg ctcaccgagt tcccgatcgc gacgatggcg cacgtcaccg
      541 actccgacga agagctcgcg cacaccgagg gtcgccccgg ccctggtgtg acgttgaagc
      601 tcgtcacgct cgacggtcgc gtcgccggcg tgaacgagga aggcgagatc cgcaccaagg
      661 gccggcagat gttccgtggc tacatggact cgtcgctcga cgacgccgcg ttcgacgagg
      721 acggctggtt ccgcaccggc gacctcggga aggtgaacga ccacggcaac gtgatcatca
      781 ccggccgcct gaaggacgtg atcatccgca agggcgagaa catctcggcg aaggaagtgg
      841 aagacctgct gttcacacat ccgaagatcg ccgacgcggc ggtgatcggg ctgccggacc
      901 cgaagctggg ggagcggtgt gtcgcggtga tcgcacccgc ggatccgggc gatccgccga
      961 cgctgcccga gctgttcgag ttctgcaagg aatcgggcct catggtgcag aagatccccg
     1021 agcagctcga gatcgtcgac gtgctgccgc gcaacccgtc gggcaaggtc ctcaagcacg
     1081 agctgcgctc gacgtactcg agctgacctg acccgaggac gagggtgggc tgcccgtagc
     1141 ctgaccgggt gcgggtccgg cgcgtcgtgg cgatgatgat cgtggtcgtc gtgatcgcgg
     1201 cgttgctgct cgaagggatc gccggcgcgc agacgaacgc ggcgccgaag ggcacgctgc
     1261 gctggcggcc ctgcggtggt tccttcgagt gcagcacgat gcgcgtgccg ctcgactacg
     1321 cgaacccgac cggcgcgcag gtggacatcg cggtgatccg actcccggcg caagacgact
     1381 cccgccggat cggctcgctg gtggtcaacc ccggcggacc gggaggttcg ggcgtcgact
     1441 tcgtcgcgag tcaggccgac tcgctccccg cagagctccg cgaccggttc gacatcgtcg
     1501 gcttcgatcc gcgcggcacc ggacagagcg acccggtgaa ctgcaaagcc gacatgaaca
     1561 cgtactaccg gctcgacttc tcgcccgacg acgacgcgga gcgcgcggcg ttggtgtcgg
     1621 gtatcgaggc gttcgtcgac gcgtgcagcc gcaacgaggg gagccgactc ccgttcctct
     1681 cgtccgagtc caccgtgcgc gacatcgacg agctgcgcgc cgcgctcggc gacaacaagc
     1741 tcaccttcct cggctactcc tacggctcgt acctcggcgc caagtacgcc gcgacgtatc
     1801 cggaccatgt gcgcgcgctc gtcctcgacg gtgccgtcga cccgtcgctc gattccaagc
     1861 agcagcaggt ggagcaagcg gctgggttcg aaggcgtgct caacggattc ctcaagtggt
     1921 gcgcgaagaa ctcgtcgtgt gacttccatc gcgacggcaa gcccgcgccc gcgctcgacc
     1981 gcttgctggc acgcgtcgac cgtgaagggg tcgccgtccc ggacacgcgg ccggcgcgca
     2041 cgctgtcgcc gatcgagttc gatctcggga tcgcgtccgc gctctacgag ggccggggcg
     2101 gcttcagctt cctcgcgtct gcgctcgacg ccgccgatcg tggggacggc cggaccatgg
     2161 ccgacctcgc ggacagctac accgagcgcc accgcgacgg ctcgtacggc aacatcgagc
     2221 aagcgttcct cgccactggt tgtgccgacg ggccgccggt gggcacggtg aacgacgtct
//
LOCUS       DASUGA010000167         2447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1427793, whole genome shotgun sequence.
ACCESSION   DASUGA010000167 DASUGA010000000
VERSION     DASUGA010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427793"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VFX21_03190"
     CDS             <1..632
                     /locus_tag="VFX21_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5094985.1"
                     /translation="YLAYNVMVANDTPRFAADLMFSRVIQHFPDITIALSEGGIGWIP
                     FLLERLEDTYSRQRAWTGDDLGPGLTPTDVYRRNFVSCFIRDRVGIEQRDLIGLETIC
                     WELDYPHSDSSWPDAPEQLLAQLDGASDDEIDAICWRNAARIYGYDAVDRLGRDACTV
                     AALRSRVDDVDLAAPKVAAGRAPVAGKEAITYGDMKQRMASVLSGGLEG"
     gene            616..1875
                     /locus_tag="VFX21_03195"
     CDS             616..1875
                     /locus_tag="VFX21_03195"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5094986.1"
                     /translation="MDSKGEPLHVSALGHVRICDLSGQLAGAGATKILAALGAEVIRV
                     ENPRNQGRWDMMRGVGPYVDDRRGPNLGGGFNNHNAGKLGVTIDLADPRGKDLFADLV
                     RISDVVTENFAAGVMERLGFGYESLRALRDDVVYVSSSGFGADGPYQPFKTWGPIVQA
                     MSGLTFTSGLPDREPAGWGFSYMDHLGAYSMALAVLVGLHARDATGEGQWIDLATTEA
                     ALTLHGPALLDWTVNGRGRRRAGQPNGNHAAYDEMAPHAAYPALGEDRWVAIACRDDD
                     DWARARSVFSAPWSDDARWTTLAARLAAQDELDEHIAMWTAARDADDIARDLIAVGVP
                     ASPVRTPPERIDHDPDVAVWGLFPTVEHPEMGSVRVEGLPIHLSETDWAYRRAAPCLG
                     EHNDFVFGELLGREADEIAGLRADGVV"
     gene            1872..>2447
                     /locus_tag="VFX21_03200"
     CDS             1872..>2447
                     /locus_tag="VFX21_03200"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5094987.1"
                     /translation="MSAQPSSPAGPLRDLRVVELAGEYTGLAGRFFADLGADVVVVEP
                     PGGATARHLPPFVDDEPHPDRSLRWWSEAANKRSVILDLDAGVDRERLRALLDGVDVV
                     IECESVARLAALELDPARELERHPGLVWVTITPFARGGPRADEPACDLTLLAGGGPMW
                     SCGYDDHDLPPIRGQGYQSHQMGAHFAAIGAL"
BASE COUNT          363 a          852 c          851 g          381 t
ORIGIN      
        1 cgtacctcgc gtacaacgtg atggtcgcca acgacacccc acggttcgcg gccgacttga
       61 tgttctcgcg cgtgatccag cacttccccg acatcacgat cgcgttgtcc gagggcggga
      121 tcggctggat tccgttcctc ctcgaacggc tcgaagacac gtattcgcgc cagcgcgcct
      181 ggaccggaga tgacctcggc ccgggcttga cgcccaccga cgtgtaccgg cgcaacttcg
      241 tctcgtgttt cattcgcgat cgcgtcggga tcgagcaacg cgacctcatc ggtctcgaga
      301 cgatctgttg ggagctcgac tatccgcact ccgacagctc gtggcccgat gctcccgagc
      361 agctcctcgc ccagctcgac ggcgcgagcg acgacgagat cgatgccatc tgctggcgca
      421 acgcggcccg catctacggc tacgacgcag tcgaccggct cggtcgggac gcctgcacgg
      481 tcgcggcact gcggagtcgc gtcgacgacg tcgacctcgc cgcgccgaag gtcgccgccg
      541 gacgcgcgcc ggttgcgggg aaggaagcga tcacgtacgg cgacatgaag cagcggatgg
      601 cctcggtgct cagcggtgga ctcgaagggt gagccgctgc acgtgagcgc gctcgggcac
      661 gttcggatct gcgatctctc gggtcagctg gcgggcgccg gcgccaccaa gatcctggcc
      721 gcgctcggcg cggaggtcat ccgggtcgag aacccgcgca accagggtcg ttgggacatg
      781 atgcgcggcg tcgggccgta cgtcgacgac cgccggggcc cgaacctcgg tggtggcttc
      841 aacaaccaca acgccggaaa gctcggcgtg accatcgacc tcgccgatcc gcggggcaag
      901 gacctcttcg ccgacctggt ccggatctcg gacgtggtca ccgagaactt cgctgccggc
      961 gtgatggaac gactcggctt cgggtacgag tcgttgcgcg cgctgcgcga cgacgtcgtg
     1021 tacgtctcga gcagcggctt cggcgccgac ggtccgtacc agccgttcaa gacctggggc
     1081 cccatcgtgc aggcgatgtc cggactgacg ttcacttcgg gtcttcccga tcgcgaaccg
     1141 gcgggatggg gcttctccta catggaccat ctgggcgcgt actcgatggc cctggccgtc
     1201 ttggtgggac tccacgcgcg cgacgcgacg ggcgaagggc agtggatcga cctcgccacc
     1261 accgaggccg cgctcacgct gcacggcccc gcgttgctcg actggaccgt gaacggtcgc
     1321 ggccgccgcc gcgccgggca gcccaacggg aaccacgctg cgtacgacga gatggcgccg
     1381 cacgcggcgt acccggccct gggcgaagac cgctgggtcg cgatcgcctg ccgtgacgac
     1441 gacgactggg cgcgagcacg ctcggtgttc tccgcaccct ggagtgacga cgcgcgatgg
     1501 acgaccctcg ccgcgcggct cgccgcgcaa gacgagctcg acgagcacat cgcgatgtgg
     1561 actgccgcac gcgacgccga cgacatcgcg cgcgacctca tcgccgtcgg tgtgcctgcg
     1621 agcccggtgc gcacgcctcc tgagcggatc gaccacgatc ccgacgtcgc ggtgtggggg
     1681 ctcttcccga cggtcgagca tcccgagatg ggaagcgtgc gggtcgaggg actgccgatc
     1741 catctctctg agaccgactg ggcgtaccgc cgcgcggcac cgtgtctcgg agagcacaac
     1801 gacttcgtgt tcggtgagtt gctgggccgc gaggccgatg agatcgccgg cttgcgcgcg
     1861 gacggagtgg tgtgagcgcg caaccatcgt cgccagcggg gccgctgcgc gacctgcgcg
     1921 tcgtcgagct ggcgggcgag tacacggggt tggcgggccg cttcttcgcc gatctcggcg
     1981 ccgacgtcgt ggtcgtcgaa ccgcccggcg gcgcaaccgc gcgccacctg ccgccgttcg
     2041 tcgacgacga gccccatccc gaccgcagtc tgcggtggtg gtcggaggct gccaacaagc
     2101 gcagcgtgat cctcgatctc gatgctggcg tcgatcgcga acggctccgg gcgctgctcg
     2161 acggtgtcga tgtcgtcatc gagtgcgagt cggtcgcgcg tctcgcggca ctcgagctcg
     2221 atccggcgcg agagctcgaa cgtcatcccg gtctcgtgtg ggtgacgatc acgccgttcg
     2281 cgcgtggcgg tccccgcgcc gacgaacctg cgtgcgacct cacgctgctc gccggcggag
     2341 gcccgatgtg gagctgcggc tacgacgacc acgatctccc gccgatccga gggcagggct
     2401 accagtcgca ccagatgggg gcgcacttcg ccgccatcgg cgcgctc
//
LOCUS       DASUGA010000168         9012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_455993, whole genome shotgun sequence.
ACCESSION   DASUGA010000168 DASUGA010000000
VERSION     DASUGA010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9012
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455993"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1672
                     /locus_tag="VFX21_03205"
     CDS             <1..1672
                     /locus_tag="VFX21_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094988.1"
                     /translation="PRADAAGIVTHAWMGLDAIDRVQDPQLHALLDAHRDQVRAGAEF
                     PDGGYWTRAQGTPGGDYGEEAHWQRFVDAYTAQIRNDPACGDLTDPNGPCAPTIAHLM
                     GAAAHGMGDEVWDWLFEPNGPGFDESYLPPDFTPFVGPGGLELQLDIVAIARHGRPTG
                     PTPAIPDPAKIDAAFAAVGRGDIDPSSFDVGEGFLDVERAAEAFWAPRHIDALERAMP
                     WTSYNMTRTGGGVDFAARAISGYFETIWANLLGQPHTTHVAATAPYDGQRNVPYAGWT
                     GNYSPGSNAGNSGGLTRIAAALSSALPYHALAGQGSQPSELPAGSLRLRDLTSGQLVP
                     ARAGYPRIVPYNPEAGEHVIAFQPAADLQPCRWYRVETTTALVDAQAQSVAPAQWQFR
                     TSGCTPPQLKLPVHGTVHCDASGSARFTRIGDQANVRLALTNCSGGQDGTPQHRKATM
                     NIASGRVHVQLSLPTSSCSELTEPTAPSSLTGTVRWYDAAGRTIGMSRVAPQAFDTRG
                     AVVRLAGAFVLDRMAVRWTPDVTGCTGSTATETVPLTAGHVTVFPQFA"
     gene            complement(1691..2140)
                     /gene="rplI"
                     /locus_tag="VFX21_03210"
     CDS             complement(1691..2140)
                     /gene="rplI"
                     /locus_tag="VFX21_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013155831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX5094989.1"
                     /translation="MKILLREDVDNLGNKGDIVEVADGYARNYLVPRGLAMPATRGIQ
                     KQADAMRRNRAAKDKRDREQAEAVAARFSGRTVTVEARAGEGGKLFGSITASDIADAV
                     LAQVGVEIDRRRIALEEPLKEIGSVELAVRLHPDVTAPILVEVVAAS"
     gene            complement(2339..2590)
                     /gene="rpsR"
                     /locus_tag="VFX21_03215"
                     /pseudo
     CDS             complement(2339..2590)
                     /gene="rpsR"
                     /locus_tag="VFX21_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563835.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
     gene            complement(2603..3085)
                     /gene="ssb"
                     /locus_tag="VFX21_03220"
     CDS             complement(2603..3085)
                     /gene="ssb"
                     /locus_tag="VFX21_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX5094990.1"
                     /translation="MANNTNSITIVGNITRDPEMRYTAGGTSTATFGVAVNRSWRNQQ
                     TNEWEERTSFFNVVCWRQLAENVSASLRKGTRVIVTGRLEQRSWETEQGEKRSVVEVV
                     ADDVAPSLVFATAEVHRVERSGPDGAPAPRQQSPQQAAPVGGGASGGGNYDDYGEEPF
                     "
     gene            complement(3095..3448)
                     /gene="rpsF"
                     /locus_tag="VFX21_03225"
     CDS             complement(3095..3448)
                     /gene="rpsF"
                     /locus_tag="VFX21_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005467069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX5094991.1"
                     /translation="MPSALRPYEVMIILDADLDEETIRAAVERWLQLIEARGAERGHL
                     DVWGKRRLAYEINRKTDGFYVVFQAKSEPAAMEELHRVLSLADEVIRHKVLRIPAKVY
                     GPPQNAGNAAVAEHS"
     gene            complement(3728..4249)
                     /locus_tag="VFX21_03230"
     CDS             complement(3728..4249)
                     /locus_tag="VFX21_03230"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX5094992.1"
                     /translation="MSNRLGIAIAIAFLVVVLTALSVWVLRDVVKNVEASVEIDGVDT
                     STAAVAPFAGLTEANVSVDGDCKRVAVADSLAERQEGLRGATDLTPYDGMLFVFASDS
                     DSHFTMADTLVPLDIGWYSANGLPVDRTTMQPCPEGDDATCPDYGATSKYRYALETPA
                     GQLGGGSLSGCSS"
     gene            complement(4386..5273)
                     /gene="panB"
                     /locus_tag="VFX21_03235"
     CDS             complement(4386..5273)
                     /gene="panB"
                     /locus_tag="VFX21_03235"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX5094993.1"
                     /translation="MSANARAARVTAPSILARKHDDTPLVMVTAYDATSARTADAADV
                     DMLLVGDSLAMVVLGYDDTLQVTTDDMAHHVGAVARTKPRALIVGDLPWMSYHVSRED
                     TVRNAATLVRAGAGAVKLEGGRKRIDSISAILDAEIPVMGHIGLTPQSIHALGGFKVQ
                     GKQLDAARRLVDDAVALADAGVFAIVLECVPDGVARMVTETVPVPTIGIGAGRHCDGQ
                     VLVWHDVLGFTAPGARVPKFVRQYATMEADASIAIAAFARDVREGKFPSSAETYHMTD
                     QMADALGLYGASGMEPVPQ"
     gene            5405..5605
                     /locus_tag="VFX21_03240"
     CDS             5405..5605
                     /locus_tag="VFX21_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094994.1"
                     /translation="MTARVCENCAFADDELVLVRRVYVTPETWDQPGSHQVVPEPELW
                     CVSCVTQYPCELADEEDEDEDT"
     gene            complement(5710..6072)
                     /locus_tag="VFX21_03245"
     CDS             complement(5710..6072)
                     /locus_tag="VFX21_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HEX5094995.1"
                     /translation="MYRVTDYALARRAVLRDFRSGALTQLDVCDAHPELLRAAHNIGT
                     ELTDECPVCGEARLRLVSYVYGEKLKTANGRCVADDELAKLGASYDEFACYDVEVCIE
                     CRWNHLRRQSLHGRLHAS"
     gene            6284..6901
                     /locus_tag="VFX21_03250"
     CDS             6284..6901
                     /locus_tag="VFX21_03250"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX5094996.1"
                     /translation="MLELAVLGILAEQPLHGYELKKRLSETLGPLWGISFGSLYPALR
                     RLERDGSIVQTGAVETGAAAFVPTGSLDGDLAAARRRARPRPSRRTKKAYTITDAGRE
                     AFTRLLETDEPDDERTFALKLSFCRYMTTDARLEFLGRRRSELAQRLSKSRRTPTTRT
                     IDRYTRSLLEHRAASTQHEIQWIDELIAEERANQIAERTEGAASA"
     gene            6898..7986
                     /locus_tag="VFX21_03255"
     CDS             6898..7986
                     /locus_tag="VFX21_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX5094997.1"
                     /translation="MSGPTASNKVRVAIAGVGNCASSLVQGVEFYRNADPNERVPGLM
                     HVELAGYHVGDVEFVAAFDVDAEKVGLDLGKAIFTSINNTIKFADVGELGVLVQRAPT
                     LDGLGKYYREVVEESAAPPVDVAQALRDARADVLVSYLPVGSEEAQRMYAQACLDAGV
                     GFVNAIPVFIASNPEWARRFEAAGVPIVGDDIKSQVGATITHRLLARLFEDRGLAIDN
                     TYQLNFGGNMDFKNMLERERLLSKKVSKTQSVTSQIDQGISSESVHIGPSDHVPWLND
                     RKWAYIRLEGRNFGDVPLNVELKLEVWDSPNSAGVIIDAVRCCKVAKDRGIGGPLLGP
                     SAYFMKSPPVQYHDDEAHRMVEDFANGA"
     gene            complement(8048..8857)
                     /locus_tag="VFX21_03260"
     CDS             complement(8048..8857)
                     /locus_tag="VFX21_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5094998.1"
                     /translation="MKFGSTRVRALGAALMVVALGTTLVACGGDDDDDDAASSDTTAA
                     AEGASADVAEVCDINIQVGAATAPIFEAEDPEGVKAAYAESGVADLFDQVEALDLPAE
                     LSGPLGEAMDTIRTAGETGDTSGLQDFDTTDIDAWFYDNCDYQQVPVTAKEYEFDGLP
                     ATLDAGATSFKLTNEGEEMHEIVIARKADGVTDSWDDILALPEEEAMAKVSIAGAAFA
                     PIGQTGYFATDLDTGDYVAVCFIPQGTKSFEDQLDAPPHMVLGMKHEFTVS"
BASE COUNT         1361 a         3136 c         3056 g         1459 t
ORIGIN      
        1 gccgcgcgcg gacgcggcgg ggatcgtcac gcatgcgtgg atggggctcg acgcgatcga
       61 tcgcgtccaa gacccacagt tgcacgcgtt gctggacgcg catcgcgatc aggtgcgggc
      121 cggtgccgag tttcccgacg gcggctactg gacacgcgcc caagggacgc cgggcggcga
      181 ctacggcgag gaagcgcact ggcagcggtt cgtcgacgcg tacaccgcgc agatccgcaa
      241 cgatcccgcg tgcggagacc tgaccgaccc gaacggtccg tgcgcgccga cgatcgcgca
      301 tctcatgggc gcagccgcgc acgggatggg cgacgaagtg tgggactggt tgttcgagcc
      361 gaacggtccc ggcttcgacg agtcgtacct gccaccggac ttcacgccct tcgtcgggcc
      421 cggcggtttg gagctccagc tggacatcgt cgcgatcgcg cgtcatgggc ggccgaccgg
      481 tccgacaccg gcgatcccgg acccggccaa gatcgacgcc gcgttcgccg cggtcggccg
      541 cggcgacatc gacccgagca gcttcgacgt gggcgagggc ttcctcgacg tcgaacgggc
      601 cgcggaagcg ttctgggcac cccgtcacat cgacgcgttg gagcgcgcga tgccgtggac
      661 ctcgtacaac atgacgcgga ccggtggcgg tgtcgacttc gcggcgcgtg cgatctccgg
      721 ttacttcgag acgatctggg cgaacttgtt gggacagccg cacacgacgc acgtcgcggc
      781 gacggcgccc tacgacgggc aacgcaacgt tccgtacgcg ggctggaccg gcaactactc
      841 gcccggctcg aacgcgggga acagcggcgg gctcacgcgc atcgcggccg cgctgtcgag
      901 cgcgctcccg taccacgcgc tcgcgggcca gggttcgcag ccgagcgagc tgccggccgg
      961 ttcgctgcgg ctgcgcgacc tgacgtcggg acagctcgtt cccgcgcgcg ccggctatcc
     1021 gcgcatcgtg ccgtacaacc cggaagcggg tgagcacgtc atcgccttcc agcccgcggc
     1081 cgacctgcag ccgtgccgtt ggtaccgcgt cgagaccacg accgcgctcg tcgacgctca
     1141 ggcgcaatcg gtggcgcccg cgcagtggca gttccgcacg agcggctgca cgccgccgca
     1201 actgaagttg ccggtgcacg ggaccgtgca ctgcgacgcg tccggatcgg cgcgcttcac
     1261 acggatcggt gatcaggcca atgttcgctt ggccctgacg aactgctccg gcggtcagga
     1321 cggcacgccg cagcaccgca aagcgacgat gaacattgcc tcgggtcggg tgcacgtaca
     1381 gctctcactc ccgacgtcgt cgtgttccga gttgacggag ccgactgcgc cgtcgtcgct
     1441 cacgggcacc gtccgctggt acgacgcggc cggccgcaca atcggcatga gccgggtggc
     1501 gccccaggcg ttcgacacac ggggcgccgt cgtccgcctc gcgggcgcgt tcgtcctgga
     1561 ccgcatggcc gtgcgctgga cgcccgacgt caccggatgc accggctcga ccgcgaccga
     1621 aacggtgccc ctgaccgcgg gccacgtcac cgtcttcccg cagttcgcct gacgcgaaga
     1681 acgggcggag ctacgaggcg gcgacgactt cgaccaggat gggtgcggtg acgtcggggt
     1741 gcaggcgtac cgccaactcg acgctgccga tctccttgag cggctcctcg agcgcgatgc
     1801 ggcggcggtc gatctcgacg ccgacctgcg cgagcaccgc gtcggcgata tcggacgcgg
     1861 tgatcgagcc gaacaacttg ccgccctcgc ccgcgcgcgc ctcgaccgtc accgtgcggc
     1921 ccgagaagcg ggcggcgacg gcctcggcct gctcgcggtc acgcttgtct ttcgcggcgc
     1981 ggttgcgacg catcgcgtcc gcttgcttct ggatgccacg cgtcgccggc atcgcgagcc
     2041 cgcgaggcac gaggtagttg cgcgcgtaac cgtcggcaac ctcgacgatg tcgcccttgt
     2101 tgccgaggtt gtcgacgtct tcgcgaagca ggatcttcat cgcttattcc tcacccgctt
     2161 cggccgcgac cgtctccatc tcctcggcgc cttcgtaggt gtcttccgcg ccttcgagct
     2221 cgccggtcgt caactcggtc tcgtcctggg cctgcaactc gcgctcgccc cgatccggac
     2281 ggtcaccgcg ctcaccgcga tcgcgccggc ccttcgaccg ctgcgtgacc tgacggacgc
     2341 tgtagggcaa cagcgccatc tcgcgtgcga gcttgatcgc acgcgcgacg gcggcctgct
     2401 gcttctggtc gttcccggtg acgcgccgag cgcggatctt ggcgcgctcc gacatgaacc
     2461 ggcggagcag gttcacgtcc ttgtagtcga tccactcgat gctctcggtg gtgaggatcg
     2521 acgtcttctt cttgatggga cggcggtctt tggacgccgg ccccttcttc tcgttcctgc
     2581 gctgtgccat tcctgctccg gatcagaacg gctcttcgcc gtagtcgtcg tagttgccgc
     2641 cgccgctagc gccgccgccg accggtgccg cctgctgcgg tgactgctgg cgcggggcgg
     2701 gcgcgccgtc ggggccgctg cgctcgacgc ggtgcacttc cgcggttgcg aacacgaggc
     2761 tcggcgcgac gtcgtcggcg acgacctcga cgaccgaacg cttctcgccc tgctcggtct
     2821 cccagctgcg ctgctcgagc cggccggtga cgatgacgcg cgtgcccttg cgcaacgacg
     2881 cggagacgtt ctcggcgagc tgacgccagc acacgacgtt gaagaagctc gtgcgctctt
     2941 cccactcgtt ggtctgctgg ttgcgccacg agcggttcac ggcgacgccg aaggtcgcgg
     3001 tgctcgtgcc gcccgcggtg tagcgcatct cgggatcgcg ggtgatgttg ccgacgatcg
     3061 tgatggagtt cgtgttgttg gccatggttc ctccctagga gtgctccgcc accgcggcgt
     3121 tgcctgcatt ctgaggaggt ccgtagacct tcgcggggat gcgcaacacc ttgtgccgga
     3181 tgacttcgtc tgcaagggag agaacccggt gcagctcttc catggccgcc ggctccgact
     3241 tggcctggaa gaccacgtag aagccgtcgg tcttgcggtt gatctcgtac gcgaggcggc
     3301 gtttgcccca cacgtcgaga tggccccgct cggcgccgcg cgcctcgatg agctgcagcc
     3361 accgttctac ggcggctcgg atcgtctcct cgtccaggtc cgcgtcgagg atgatcatga
     3421 cttcgtacgg ccgcagtgca ctgggcacgg gcaacgcctc cttcggaccg cagcctcggg
     3481 gctacgggct cctcggtgcc ggcgggccct cggagcaggg gatctcggtt gtcgaaccgg
     3541 gaaaggctag cagtcagatc tcctcgaacg tcctatcgat gcggacgcta cacaggaggt
     3601 gagacacttg tctttgtcgc tccgcacgtg tccgcgtgag ccagctcgga ttgttggccc
     3661 gcgctccgct ccgcgagtgc ctccgcgagc tccggcaccg cctcgtgcgg ccggccggga
     3721 gcggtttcta cgaggagcag ccggagaggg agccgccgcc gagctggccg gcgggggttt
     3781 cgagcgcgta gcggtacttg ctcgtcgccc catagtccgg gcacgtggcg tcgtcgcctt
     3841 cggggcaggg ctgcatcgtg gtgcgatcga ccgggagccc gttcgccgag taccaaccga
     3901 tgtcgagcgg caccaatgtg tccgccatcg tgaagtgcga atcggagtcg gacgcgaaca
     3961 cgaacagcat gccgtcgtag ggtgtgaggt cggtcgcacc ccgcaagccc tcttgacgtt
     4021 ccgcgagcga gtctgcaacg gcgacgcgct tgcagtcgcc gtcgacggag acgttcgctt
     4081 cggtcaatcc ggcgaacggc gcgaccgccg cggtcgacgt gtcgacgccg tctatctcta
     4141 cagatgcctc gacgttcttc acgacgtcgc gcagcaccca cacgctgagt gcggtgagca
     4201 cgacgacgag gaacgcgatc gcaatcgcga taccaaggcg gttggacact cccgcaagct
     4261 actgcggcac cggctccatc tctctttgtc gctccgcacg tgctcgcgtg aggcagcccg
     4321 gattgttggc ccgcgctccg ctccgccagt gcctccgcaa gctccggcac cccgcgtgtt
     4381 ggcgcttatt gcggcaccgg ctccattccg ctggcgccgt agaggccgag ggcgtcggcc
     4441 atctggtcgg tcatgtggta cgtctcggcg ctcgacggga acttgccctc gcgcacgtcg
     4501 cgggcgaacg ccgcgatcgc aatcgaggcg tcggcctcca tcgttgcgta ctggcgcacg
     4561 aacttcggca cccgcgcccc cggcgcggtg aagccgagca cgtcgtgcca cacgagcacc
     4621 tggccgtcgc agtggcggcc ggcaccgatg ccgatcgtgg gcaccggcac ggtctcggtg
     4681 accatgcggg cgacgccgtc ggggacgcac tccagcacga tcgcgaacac acccgcgtcc
     4741 gcgagcgcga ccgcgtcgtc gaccaatcgg cgtgcggcat cgagctgctt gccctgcacc
     4801 ttgaagccac cgagcgcgtg gatcgactgc ggcgtgagac cgatgtggcc catcaccggg
     4861 atctccgcgt cgaggatcgc cgagatcgaa tcgatgcgct tgcggccgcc ttcgagcttc
     4921 accgcgccgg caccggcgcg cacgagcgtc gccgcgttgc ggacggtgtc ttcgcggctg
     4981 acgtggtagc tcatccacgg caggtcgccg acgatcaacg cacgcggctt cgtgcgcgcg
     5041 acggcgccga cgtggtgcgc catgtcgtcg gtggtcactt gcagcgtgtc gtcgtagccg
     5101 agcacgacca tcgcgagtga gtcgccgacg agcagcatgt cgacgtccgc ggcgtcggcc
     5161 gtgcgcgccg acgtcgcgtc gtacgcagtg accatgacga gcggcgtgtc gtcatgcttg
     5221 cgggccaaga tggacggcgc cgtgacgcgc gccgcgcgag cgtttgctga catctccgca
     5281 cctccgtcgt ccagggctcc acagctcccg gcaacggctc caggctaccc aagcagcccg
     5341 gcgaacgcac ctccgccccc ccgggcatgt gacggtgatc acgatcgcgg gcgacaatgc
     5401 gggcgtgacg gcacgggtct gcgagaactg cgcgttcgcc gacgacgagc tggtgctcgt
     5461 ccggcgcgtc tacgtcacgc ccgagacgtg ggaccagccc ggttcgcacc aagtcgtgcc
     5521 cgagccggag ctgtggtgcg tgtcgtgcgt gacgcagtac ccgtgcgagc tcgccgacga
     5581 agaagacgag gacgaagaca cctgaggtga ggcgcgttcg ttgcgccgac gtgcctcacg
     5641 cgcgcgcgtc gaaccggggc ccgagcggcc cttcgagcgg gaagcacgac tgaactgctg
     5701 aacgcggtgt cagctggcgt gcaggcggcc gtgcagtgac tgccggcgga gatggttcca
     5761 gcggcactcg atgcacactt cgacgtcgta gcacgcgaac tcgtcgtagc tcgcgccgag
     5821 cttggccagc tcgtcgtcgg caacgcagcg accgttcgcg gtcttgagct tctcgccgta
     5881 gacgtacgac accaagcgca ggcgcgcttc gccgcacacc ggacattcgt cggtgagctc
     5941 ggtgccgatg ttgtgcgcgg cgcgtaagag ctccggatgc gcgtcgcaga cgtcgagctg
     6001 cgtgagcgca ccgctgcgga agtcacgcag cactgcccgc cgggccaatg cgtagtccgt
     6061 gacgcgatac atcgagcgcg aagtgtagag gtgcgcgaac cactgatctt ccgcgcgcga
     6121 accgtccgcc agggcagcag tcgcacggcg cggcgccgga gcttgcgccg gcgctcgccg
     6181 gagtctttcg gcgggccaac aacccagacc gcctcacgcg agcacgccga aagcgataag
     6241 gacaagttga tctgatatat cgattcgata tatcgtggtg cacatgttgg agctggccgt
     6301 gctgggcatc ctcgccgagc agcccctgca cgggtacgag ctgaagaagc ggctgtccga
     6361 gacgctgggg cccctgtggg ggatctcgtt cggctcgctc tatccggcgc tgcgccggct
     6421 ggagcgcgac ggctcgatcg tccagaccgg cgcggtcgag accggcgccg cggcttttgt
     6481 cccgaccggc tccctcgacg gcgacctcgc cgcggcccgc cgccgggcgc ggccccggcc
     6541 cagccgccgg accaagaagg cctacacgat caccgatgcc ggccgggagg cgttcacccg
     6601 tctcctcgag accgacgagc ccgacgacga gcgcacgttc gcgttgaagc tctcgttctg
     6661 ccggtacatg accaccgacg cgcggctgga gttcctcggc cgccggcggt cggagttggc
     6721 gcagcggctg agcaagtcgc gtcgcacgcc gaccacccgc accatcgatc gctacacccg
     6781 gtcgctcctc gagcaccggg cggcatcgac ccaacacgag atccagtgga tcgacgaact
     6841 gatcgccgaa gagcgcgcga accagatcgc cgaacgcacc gaaggagcag cgtcagcatg
     6901 agcggcccca ccgcaagcaa caaagtccgt gtcgcgattg ccggggtggg caactgcgcg
     6961 agcagcctcg tccagggcgt cgagttctac cgcaacgccg atcccaacga gcgcgtcccc
     7021 ggcctgatgc acgtcgagct cgccggctac cacgtcggtg acgtcgagtt cgtcgccgcg
     7081 ttcgacgtcg atgccgagaa ggtcggtctc gacctcggca aggcgatctt cacgagcatc
     7141 aacaacacga tcaagttcgc cgacgtcggc gagctcggcg tgctcgtgca gcgcgccccg
     7201 acgctcgacg ggctcggcaa gtactaccgc gaggtcgtcg aggaatcggc cgcgccgccg
     7261 gtcgacgtgg cgcaggccct gcgcgacgcg cgtgccgacg tgctcgtgtc gtacctcccg
     7321 gtcgggtccg aagaggcgca gcgcatgtat gcgcaggcct gcctcgacgc gggcgtcggc
     7381 ttcgtcaacg cgatcccggt gttcatcgcc agcaaccccg aatgggcgcg ccgcttcgag
     7441 gcggcgggcg tgccgatcgt cggcgacgac atcaagagcc aggtcggcgc gacgatcacg
     7501 caccgcctgc tcgctcgcct gttcgaagac cgcggcctcg cgatcgacaa cacgtaccag
     7561 ctgaacttcg gcggcaacat ggacttcaag aacatgctcg agcgtgagcg cttgctgtcg
     7621 aagaaggtgt cgaagacgca gtcggtgacg agccagatcg accagggcat ctcttcggag
     7681 agcgtgcaca tcggcccgtc cgaccatgtg ccgtggctca acgatcgcaa gtgggcgtac
     7741 atccgcctcg agggccgcaa cttcggtgac gtgccgctca acgtcgagct caagctcgag
     7801 gtctgggact cgcccaactc ggccggtgtg atcatcgacg ccgtgcgctg ctgcaaggtc
     7861 gccaaggacc gcggcatcgg tgggccgctg ctcggcccgt cggcctactt catgaagtcg
     7921 ccgccggtgc agtaccacga cgacgaagcg caccgcatgg tcgaggactt cgccaacggc
     7981 gcgtagtccg tcggaacgcc gagaggggcg gcccggacgg ggccgcccct ctcgcgtcga
     8041 caccgcttca ggagaccgtg aactcgtgct tcatgccgag caccatgtgc ggcggcgcat
     8101 cgagctggtc ctcgaaggac ttcgtgccct gcgggatgaa gcagaccgcg acgtagtcgc
     8161 cggtgtccag gtcggtcgcg aagtagcccg tctgaccgat cggcgcgaac gccgcgcccg
     8221 cgatcgagac cttggccatc gcttcctctt ccggcaacgc gaggatgtcg tcccacgagt
     8281 ccgtgacgcc atcggccttg cgggcaatga cgatctcgtg catctcctcg ccctcgttgg
     8341 tcagcttgaa gctggtcgcg ccggcgtcga gcgtcgcggg caggccgtcg aactcgtact
     8401 ccttggccgt caccggcacc tgctggtagt cgcagttgtc gtagaaccag gcgtcgatgt
     8461 cggtcgtgtc gaagtcctgc agacccgacg tgtcgccggt ctcgcccgcg gtccggatcg
     8521 tgtccatcgc ctcgccgagc gggcccgaga gctccgccgg caggtcgagc gcctcgacct
     8581 ggtcgaagag gtcggcgacg cccgactccg cgtaggcggc cttcacaccc tcggggtcct
     8641 cggcctcgaa gatgggggcc gtggccgcac cgacttggat gttgatgtcg cacacctcgg
     8701 ccacgtcggc ggacgcgccc tcggcggcgg ccgtcgtgtc actcgacgcc gcatcgtcgt
     8761 cgtcgtcatc gcccccgcac gcgaccaacg tcgttccgag cgcgacgacc atcagcgcag
     8821 caccgagcgc acggactctg gtcgatccga acttcacgtg tttcctcctt gagggttgtt
     8881 cgtcaagctg accctacgga gctcgactcc cgtgacgaac cgtgtcagca ggtttcgcgc
     8941 gggagtgctg gcacacccgc gcgaagggca gaatgcacgc atggcaacga actccgaggc
     9001 gtgggatcgg at
//
LOCUS       DASUGA010000169         2172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_73125, whole genome shotgun sequence.
ACCESSION   DASUGA010000169 DASUGA010000000
VERSION     DASUGA010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73125"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1963)
                     /locus_tag="VFX21_03265"
     CDS             complement(<1..1963)
                     /locus_tag="VFX21_03265"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX5094999.1"
                     /translation="MARLWEQPEVTGIGRLPMHTPLVDRDGPWCHSLDGRWRFLYLDR
                     PEDAPAGFSDADFDVRAWDEVDVPGCWTMQGYDRPIYTNVRMPFDGAPPTVPDANPAG
                     IYRTEFSLPESWRGQRVVLHIGGAESVVFVWVNGVSLGMSKDSRLEAEFDITEHARFG
                     ARNVLTTQVVRWSDASFVEDQDQWWHAGLHRSVFVYTTPRTHIADVQVDASIADDLVT
                     GVLRATVAVDFAEDERVAGWTVHVRCETQRGRALAQLSGEVPKTRAPYVFAGHVVRLQ
                     GEVAKVAPWSAEEPVLHTLFVSLVDPDGKARERVRLRVGFRRVEILGRELLVNGEPVL
                     LRGVNRHDFDPDTGRVVTVDQMRADLVLMKQFGFNAVRTSHSPNDPRFYDLCDELGVY
                     VVDETNFESHAFIFSLCDDPRYVHALVDRGMRMVQRDKNHACIIMWSLGNESGYGAGH
                     EALAAWIRQYDPTRPLHYEGVIFTGWDRRQHVTDVLCPMYPEIADIVAWAAKEQAPAM
                     PLVMCEYSHAMGNSNGCLADYWDAIERYHGLQGGFIWEWWDHGLTQAMPDGTTRWAYG
                     GDFGDEPNDGNFCADGLVWPDRTPKPALWEHRALAAPIAISFEPAGRRLAIRNRQAVR
                     DLAWLCADWDVAVEGAVVARGTFTPPRAGP"
     gene            complement(1981..>2172)
                     /locus_tag="VFX21_03270"
     CDS             complement(1981..>2172)
                     /locus_tag="VFX21_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095000.1"
                     /translation="VLGVPVPAGHHEVKVTFTPPGLRLGAAITVASAVVCFGVVPAVI
                     TYRRRRRRTAEAAAGAPCP"
BASE COUNT          339 a          763 c          754 g          316 t
ORIGIN      
        1 ccggtccggc ccgcggcggc gtgaacgtgc cacgcgccac gaccgctccc tcgaccgcga
       61 cgtcccagtc cgcgcacagc cacgcgaggt cccgcacggc ctgccgattc cggatcgcga
      121 gtcgccgccc cgccggctcg aacgagatcg cgatgggggc agccagggcc cggtgctccc
      181 acaacgccgg cttcggcgta cggtcgggcc agaccaggcc gtcggcgcag aagttgccgt
      241 cgttcggctc gtcgccgaag tcgccaccgt acgcccagcg ggtcgtgccg tccggcatcg
      301 cctgcgtgag cccgtggtcc caccactccc agatgaagcc gccctgcagg ccgtggtaac
      361 gctcgatcgc gtcccagtag tcggccaggc agccgttgct gttgcccatc gcgtgcgagt
      421 actcgcacat gacgagcggc atcgcgggcg cctgctcctt cgccgcccac gcgacgatgt
      481 cggcgatctc cggatacatc gggcacaaca cgtcggtgac gtgctggcgc cggtcccagc
      541 cggtgaagat cacgccttcg tagtggagcg gccgggtcgg gtcgtattgg cggatccacg
      601 cggccagcgc ttcgtgaccg gcgccgtagc ccgactcgtt gccgagcgac cacatgatga
      661 tgcacgcgtg gttcttgtcg cgctgcacca tccgcatgcc gcggtcgacg agcgcgtgca
      721 cgtagcgcgg gtcgtcgcag agactgaaga tgaacgcgtg cgactcgaag ttcgtctcgt
      781 cgacgacgta cacgccgagc tcgtcgcaga ggtcgtagaa gcgcgggtcg ttgggtgagt
      841 gcgacgtgcg aacggcgttg aacccgaact gcttcatgag caccaagtcg gcgcgcatct
      901 ggtcgacggt cacgacccgg ccggtgtcgg ggtcgaagtc gtggcggttc acgccgcgca
      961 gcaataccgg ttcgccgttg accaacagct cgcggccgag gatctcgacg cgacggaagc
     1021 cgacgcgcag ccggacgcgc tcgcgcgctt tgccatccgg gtcgacgagc gagacgaaca
     1081 gggtgtgcag gacgggttct tcggccgacc acggcgcgac cttcgcgacc tcgccctgca
     1141 gccgcacgac gtgacccgcg aagacgtacg gcgcgcgcgt cttcggcact tcacccgaga
     1201 gttgcgccag cgcccggccc cgttgggtct cgcagcgaac gtgcacggtc caccccgcca
     1261 cccgttcgtc ctcggcgaag tcgaccgcca ccgtcgcgcg caacacgccg gtgacgagat
     1321 cgtcggcgat cgatgcgtcg acctgcacgt cggcgatgtg cgtgcgcggc gtcgtgtaga
     1381 cgaacaccga acggtgcagg ccggcgtgcc accactggtc ttggtcctcg acgaacgacg
     1441 catccgacca ccgcacgact tgcgtcgtga gcacgtttcg ggcgccgaag cgcgcgtgct
     1501 cggtgatgtc gaactccgct tcgaggcgag agtccttgct catgccgagc gacacaccgt
     1561 tgacccagac gaagacgacg ctctccgcgc cgccgatgtg caacacgacc cgttggccgc
     1621 gccacgactc cggcagcgag aactcggtgc ggtagatccc cgcgggattc gcgtcgggca
     1681 cggtcggcgg tgcgccgtcg aacggcatgc gcacgttcgt gtagatcggc cggtcgtagc
     1741 cctgcatcgt ccagcagccg gggacgtcga cttcgtccca cgcgcgcacg tcgaagtcgg
     1801 cgtcactgaa gccggcgggc gcgtcctcgg gccggtcgag gtacaagaac cgccaccggc
     1861 cgtcgagcga atggcaccac gggccgtcgc ggtcgacgag cggcgtgtgc atcggcagac
     1921 ggccgatgcc ggtgacttcg ggctgttccc acaggcgggc catagcccgg aagtctcgcg
     1981 ctaggggcag ggagcgccgg ccgcggcctc ggctgtgcgc cgtcggcggc ggcgatacgt
     2041 gatgactgcc gggacgacgc cgaagcacac gaccgcggac gcaaccgtga tcgcggcgcc
     2101 caggcgaagg ccgggcggcg tgaacgtcac cttcacttcg tggtggccgg ccgggacggg
     2161 aacaccgagg ac
//
LOCUS       DASUGA010000170         5775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1584968, whole genome shotgun sequence.
ACCESSION   DASUGA010000170 DASUGA010000000
VERSION     DASUGA010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584968"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..83
                     /locus_tag="VFX21_03275"
     CDS             <1..83
                     /locus_tag="VFX21_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HEX5095001.1"
                     /translation="VFALAQPPGTEVREMVVCTSTEPSWP"
     gene            80..1069
                     /locus_tag="VFX21_03280"
     CDS             80..1069
                     /locus_tag="VFX21_03280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX5095002.1"
                     /translation="MSASRRDFAPVLLALRAAGLGDLLTAIPAWRGLARAFPGYRRII
                     ATPAALAPIVTMLNAEHCEHAGLARLVCARPTVAVNLHGKGPQSHETLLATEPQALVA
                     YAHPDVPASALGPVWDDEEHEVVRWCRLLGAFDIHTDAQELGLTATAEQLATADAIVG
                     DVDDDNTTVIHPGAGSGARRWPIARYAAVARVERDAGRRVLVTGTTEERRRAARVAAG
                     GRVPSRDVVAGRTDLNALIGIVAGAGRVICGDTGVAHLATALGTPSVVLFGPTRPARW
                     GPPPSRAHIALWAGRNGNPHGRRVDPGLLEISVRDVVQALAQLPRRVVHASAS"
     gene            complement(1094..1669)
                     /locus_tag="VFX21_03285"
     CDS             complement(1094..1669)
                     /locus_tag="VFX21_03285"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5095003.1"
                     /translation="MDLAPITAFFMHAPGVPIGPFTNRATWAAHAERSERSNAMTQLN
                     HSETDVVDAIRKDHREIEQMLAAVESAQGTAKKDAFDQLARKLSAHEAAEEEVVHPLT
                     EDLGATDVAASVEMEESEASVLLSDMASMDVASSEFEAAFQKLKLAVMAHARHEESEE
                     HPRLKENESPDYLVQLADEYESAEEEAMGRA"
     gene            1634..3703
                     /locus_tag="VFX21_03290"
     CDS             1634..3703
                     /locus_tag="VFX21_03290"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEX5095004.1"
                     /translation="MHEERGNGGEIHQRANGRPRLTTQQGVPVADDQNTLRFGERGPS
                     ALEDFHFREKIFHFDHERIPERVVHARGFGAHGYFENYESLADVTRADLFQRPGERTP
                     VFVRFSTVAGNKGSFDLARDVRGFAVKLYTQEGNWDLVGNNIPVFFIQDAIKFPDLVH
                     AAKQEPDRGFPQAQTAHDNFWDFVSLMPESTHMLMWIMSDRAIPRSFRFMEGFGVHTF
                     RFVDAEGRSTFVKFHWKPLQGLQSVVWNEAVKINGADPDFHRRDLWDAIQTGNAPEWE
                     LGVQLFDDAFADEFDFDVLDATKIIPEEQVPVRRIGRLVLDRVVDNFFAETEQVAFCT
                     QNIVPGIDFTNDPLLQGRNFSYLDTQLKRLGSPNFTFLPVNAPKCPFATFQQDGHMTV
                     SNPTSRANYEPNSWPESIGGPREDPETGFTSYPATEGGDKRRVRAELFADHYSQARQF
                     YVSQTPVEQQHIADAFVFELSKVQRPDIRVRMVANLLNVDPDLAATVSAGIGIREQIE
                     ASTPARAPITDLEPSPALSIIMNGPDSIAGRKIGVLVTDGVDKELLTQLQGMTAAQGA
                     LVELVGPRVAGVETQRGDLITTTQMIEGGPSVLYDVVAIMGAAPGIDALAENAAAKDF
                     VTDAFAHCKFVGFTAGSTPLLDAAGVPYQSDDGFVPIDDLAAIATFLDRAGQLRTWAR
                     EALVKQT"
     gene            complement(3705..4259)
                     /locus_tag="VFX21_03295"
     CDS             complement(3705..4259)
                     /locus_tag="VFX21_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014350215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HEX5095005.1"
                     /translation="MGFASDVVVREIDDETWELTEALVYKGNTDTFVVKNGFRTDFAS
                     VPRAFVWLIPSYGRYTKAAILHDFLCEESKAGRFDRNDADGVFRRAMRELGVSFLRRW
                     MMWGAVSLATQWIAIRRGHPALKRIALLIVIGLPATLFFVVPAAVITVWLLLFWILEL
                     VVWLVWRPFAKRKMDPPRFIWKMS"
     gene            complement(4329..>5775)
                     /locus_tag="VFX21_03300"
     CDS             complement(4329..>5775)
                     /locus_tag="VFX21_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX5095006.1"
                     /translation="VLNNLFKHTSAQTTFPVNMVALVDGVPRTVNLQEALQGWLDHQV
                     VVVTRRTQYRLEKAEARLHIVEGLVKALDMIDAIVKAIRASKDRAEARTKLMAKPFDF
                     SEIQANHILDMPLGRLTQLGREELEKERKELVALVKELKRILAKKDVLMGVIKDELVE
                     IRDAHKAPRRTQIVGDDTGTIDVVALVEDEPYVVTITARGYVRAVPEKTRKALVANAG
                     ERDAVAQVIDTTALASVLFFTDRGRAYISTVHELPKEKLTAAQNLFQFGDGEKVVAVV
                     DGRLHDDHPHLVFVTAQGGVKRTALTEFTEASGRQAGIVAMKVAADDRIVSVFPGWDE
                     FELLLVTANGQGIRFPEEEVRPVGRSAGSMRGIKLKADDVCIGGCAVAHEEIVVIATE
                     QGFAKRTDVDEFPVQARGGSGLKAAKIDKARGKLVAVATFADDMAFLTADSVMVVPGQ
                     GVRAAGREGGGSKTAPDGLVKVVGIAPPREE"
BASE COUNT          953 a         1966 c         1818 g         1038 t
ORIGIN      
        1 tggtgttcgc cctggcccag ccaccgggca ccgaagtccg ggagatggtg gtgtgcacct
       61 ccacggaacc gtcgtggccg tgagtgcgtc gcgtcgcgat ttcgcgccgg tgttgctcgc
      121 gctgcgcgcc gccggcttgg gcgacctgct cacggcgatt cccgcgtggc gcgggttggc
      181 acgtgcgttc ccgggctatc gccgcatcat cgccacgccc gccgcactcg cgcccatcgt
      241 cacgatgctc aacgccgagc actgcgaaca cgcgggcctc gcacgactgg tgtgcgcgcg
      301 gccgaccgtc gcggtgaacc tgcacggcaa ggggccacag agccatgaga cattgctggc
      361 aactgaaccg caagccctcg tcgcctacgc ccaccccgac gttccggcgt ctgcgctcgg
      421 gccggtatgg gacgacgaag aacacgaagt ggtgcgctgg tgccggctgc tcggagcctt
      481 cgacattcac accgacgcgc aggagctcgg cctgaccgcg acggcagagc agcttgcgac
      541 tgccgacgcg attgtcggcg acgtcgacga cgacaacacc accgtgatcc atcccggtgc
      601 gggttccggg gcacggcgct ggccgatcgc acggtacgcc gcggtcgcac gtgtcgaacg
      661 tgatgcaggc cggcgcgtgc tggttaccgg cacgacggag gaacgccggc gcgccgcgcg
      721 cgtcgcggcc ggcgggcgtg tcccgagccg ggacgtcgtt gccggccgca ccgatctcaa
      781 cgcgctcatc ggcattgttg caggggcggg tcgtgtcatc tgcggggaca ccggtgttgc
      841 ccatctcgcc accgcgctcg ggacgccgtc ggtcgtgttg ttcgggccga cgaggcctgc
      901 gcgctggggt ccgccaccgt cgcgcgcgca catcgcactg tgggccggcc ggaacggtaa
      961 tccccacggc cggcgcgtcg acccgggtct gctcgagatc agcgtgcgtg atgtcgtgca
     1021 ggcattggca caacttcccc gccgtgtcgt gcacgcgtca gcatcctgat tgatccgact
     1081 atctcacgcc atctcacgcg cggcccatgg cctcctcttc ggccgattcg tactcgtcgg
     1141 cgagttgcac gaggtagtcc ggcgattcgt tctccttgag ccgcgggtgc tcttcgctct
     1201 cttcatgccg cgcgtgcgcc atgaccgcca gcttgagctt ttggaacgct gcctcgaact
     1261 cggacgacgc gacgtccatg gacgccatgt ccgacaacag cacactcgct tcggactcct
     1321 ccatttcgac gctggccgcg acgtcggttg cgccgaggtc ttcggtcagc ggatggacga
     1381 cctcttcctc ggctgcttcg tgggccgaca gcttccgcgc cagttggtcg aacgcgtcct
     1441 tcttcgctgt tccttgcgca ctctccaccg ccgcgagcat ctgttcgatt tcccgatggt
     1501 ctttgcggat cgcgtcgacg acgtcggttt cgctgtgatt cagttgtgtc attgcgttac
     1561 tcctttccga tcgctccgca tgcgctgccc atgtcgcgcg attcgtaaac ggtccaatgg
     1621 gtactcccgg ggcatgcatg aagaacgcgg taatgggggc gagatccatc aacgcgcgaa
     1681 cggccggcct cgcctgacga cgcaacaggg agttccggtc gccgatgacc agaacacgtt
     1741 gcgtttcggc gagcgcggtc cgagcgcact cgaggacttc cactttcggg agaaaatctt
     1801 ccacttcgac cacgaacgca ttcccgagcg cgtggttcac gctcgaggct tcggcgcgca
     1861 cggctacttc gagaactacg agtcactggc ggacgtgaca cgcgccgacc tgtttcagcg
     1921 gcccggcgag cgcacgcccg tgttcgtacg attctcgacc gtcgcgggca acaaagggtc
     1981 gttcgacctg gcgcgggacg tgcgcggctt tgcggtgaag ctctacacgc aggaaggcaa
     2041 ctgggacctc gtcggcaaca acatcccggt gttcttcatc caggatgcga tcaagtttcc
     2101 ggacctggtt cacgccgcga agcaggagcc tgatcgcggc ttcccgcaag cacaaaccgc
     2161 gcacgacaac ttctgggact tcgtctcgct catgcccgag tcgacccaca tgctgatgtg
     2221 gatcatgtcc gatcgagcga tcccccgttc gttccgattc atggaaggtt tcggcgttca
     2281 cacgttccgc ttcgtcgacg ccgaaggccg ctcgacgttc gtgaagttcc attggaaacc
     2341 gctgcaagga ttgcaatccg tcgtgtggaa cgaagcggtg aagatcaacg gcgccgatcc
     2401 cgattttcat cgccgtgatc tgtgggacgc gattcagacc ggaaacgccc ccgagtggga
     2461 actgggcgtc cagctcttcg acgacgcgtt cgcggacgag ttcgactttg acgtgctcga
     2521 cgcaaccaag atcattcccg aggagcaggt tccggttcgc cgcatcggac ggctcgtact
     2581 ggaccgcgtc gtcgacaact tcttcgccga gaccgagcag gtcgcgttct gcacacaaaa
     2641 catcgtgccc ggcatcgact tcacgaacga cccgttgctg caaggtcgca acttttcgta
     2701 cctcgacacg cagctcaagc ggctcggcag ccccaacttc acgttcctcc cggtcaacgc
     2761 gcccaagtgt ccgttcgcga ccttccagca ggacggccat atgacggtct cgaatcccac
     2821 ctcacgcgcg aactacgaac cgaattcctg gcccgagagc atcggcggcc cgcgcgaaga
     2881 tcccgagaca ggcttcacgt cgtatcccgc gacggaaggc ggcgacaagc ggcgtgtacg
     2941 cgccgagctc ttcgcagatc actacagcca ggcccggcag ttctacgtga gccagacgcc
     3001 agttgaacaa cagcacatcg ccgatgcgtt cgtgttcgag ctcagcaagg tccagcgtcc
     3061 ggatatccgc gtgcggatgg tcgcgaacct gctgaatgtt gaccccgatc tcgccgccac
     3121 ggtcagcgcc ggcatcggca tccgggaaca gatcgaagcg tcgacgccgg ctcgcgcgcc
     3181 gatcacagat ctcgagcctt ctccggcgtt gagcatcatc atgaatggtc cggattcgat
     3241 agcgggccga aagatcggcg tcttggtcac cgacggcgtc gacaaggaat tgctgaccca
     3301 gctgcaggga atgacagccg cgcagggcgc gctcgtcgag ctggtcgggc cgcgggtcgc
     3361 cggcgtcgag acgcagcgcg gcgacctgat caccacgacg cagatgatcg aaggcggccc
     3421 ctcggtgctg tacgacgtgg tcgcgatcat gggcgcggcg cccggcatcg acgcgctggc
     3481 cgagaacgcg gccgccaagg actttgtgac tgacgcgttc gcgcactgca agttcgtcgg
     3541 cttcacggcg ggctcgacac ccctgctcga cgcggccgga gttccctacc agagcgacga
     3601 cggcttcgtg ccgatcgacg atctcgccgc catcgcgacg ttcctcgacc gtgccgggca
     3661 actcaggacc tgggcccggg aagcgctcgt gaagcagacc tgagctacga cattttccag
     3721 atgaagcggg gtgggtccat cttgcgtttc gcgaacgggc gccagacgag ccagaccacg
     3781 agctcgagga tccagaacag caggagccag accgtgatca ccgctgccgg cacgacgaag
     3841 aacaacgtgg ccggcaggcc gatcacgatg agcagcgcga tgcgcttcag tgcggggtga
     3901 ccgcgccgga tcgcgatcca ttgggttgcg agcgagaccg cgccccacat catccagcgc
     3961 cgcaagaatg acacgccgag ttcccgcatc gcgcgccgga agacaccgtc cgcgtcgttg
     4021 cgatcaaagc gccccgcctt cgactcctcg cagaggaagt cgtgcaggat cgccgccttt
     4081 gtgtaccggc cgtacgacgg aatcagccac acgaatgccc gcggcaccga tgcgaagtcg
     4141 gtgcggaatc cgttcttgac gacgaacgtg tcggtgttgc ccttgtagac gagcgcctcg
     4201 gtcagctccc acgtctcgtc gtcgatctcc cgcaccacga cgtccgatgc aaaccccatc
     4261 gcgggacctc ccgatcgaac ctgcgtccat cagccggatg tcatccggcc agcggacgca
     4321 ggttggcgtt actcctcgcg gggtggggcg atgccgacga ctttgaccag gccgtcgggc
     4381 gcggtcttgc tgccgccgcc ctcgcggccc gcggcgcgca ccccttggcc gggcacgacc
     4441 atgaccgaat cggcggtgag gaacgccatg tcgtcggcga aggtggcgac cgcgaccagc
     4501 ttgccgcgcg ccttgtcgat cttcgccgcc ttgaggccgc tgccaccgcg agcttgcacc
     4561 gggaactcgt cgacgtcggt gcgcttggcg aacccctgct cggtggcgat cacgacaatc
     4621 tcttcgtgcg cgactgcgca gccgccgata cacacgtcgt cggccttgag cttgatgccg
     4681 cgcatcgaac cggccgagcg gccgaccggt cgcacttcct cttccgggaa ccggatgcct
     4741 tggccgttgg cggtgacgag cagcaactcg aactcgtccc agcccgggaa caccgacacg
     4801 atccggtcgt cggccgcgac cttcatcgca acgatgccgg cctgccggcc cgacgcctcc
     4861 gtgaactcgg tgagtgccgt gcgcttcacg ccgccctggg cggtgacgaa caccaaatgt
     4921 gggtggtcgt cgtgcaagcg gccgtcgacc accgcgacga ccttctcgcc gtcaccgaac
     4981 tggaacagat tttgcgccgc ggtgagcttc tccttgggca gctcgtgcac cgtcgagata
     5041 taggcgcgcc cgcggtcggt gaagaacaac acgctcgcga gcgcggtggt gtcgatgacc
     5101 tgcgcgacgg cgtcgcgctc ccccgcgttc gcgacgagcg ccttgcgcgt cttctcgggc
     5161 accgctcgca cgtagccccg agcggtgatg gtcacgacgt acggctcgtc ttccacgagc
     5221 gcgacgacat cgatggttcc cgtgtcgtcg ccgacgatct gggtgcggcg cggcgccttg
     5281 tgcgcgtcgc gtatctcgac gagttcgtcc ttgatcacgc ccatcagcac atccttcttg
     5341 gcgaggatgc gcttcagctc cttgaccagc gcgacgagtt ccttgcgctc cttctccaac
     5401 tcttcacggc cgagttgtgt gagacggccg agcggcatgt cgagaatgtg gttcgcttgg
     5461 atctcgctga agtcgaacgg cttcgccatc aacttcgtgc gcgcctcggc ccggtccttc
     5521 gacgcacgga tcgccttcac gatcgcgtcg atcatgtcga gcgccttcac gagcccttcg
     5581 acgatgtgca gccgcgcttc ggccttctcc aggcggtatt gcgtgcggcg cgtgacgacg
     5641 acgacttgat ggtcgagcca gccctgcaac gcctcttgca aattgaccgt gcgcggcacg
     5701 ccgtcgacga gcgccaccat gttgacgggg aacgtcgtct gcgcgctcgt gtgcttgaac
     5761 aagttgttca gcacg
//
LOCUS       DASUGA010000171         3436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_491192, whole genome shotgun sequence.
ACCESSION   DASUGA010000171 DASUGA010000000
VERSION     DASUGA010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491192"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..362)
                     /locus_tag="VFX21_03305"
     CDS             complement(3..362)
                     /locus_tag="VFX21_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095007.1"
                     /translation="MRHDTDGMKCGPRTRHPGRVIRRPRAAHHASDEQIMEPEVEILG
                     AHQYEIRVRGRLTPSLATEFEQLALTTSIAPVETVLAGTVDDQAALHGLLRRIEALGL
                     ELVDVRRCAALPAHDHA"
     gene            390..1670
                     /locus_tag="VFX21_03310"
     CDS             390..1670
                     /locus_tag="VFX21_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095008.1"
                     /translation="MAAPDVRVNSRRVPRVVMGFMVVVTALVVGISATGLWMRGVVFR
                     ESRWKQTIAPLGSDDEVQAALAAWAGTEIRQVIDVKPYLEDILPSGAQGLASPLSFAI
                     EGFIAERAASFFESDQFSELWERSTVRLHSAVLKVLRGDSEAVSVRDGQVVLNLVPIL
                     NQVLASISDELSQLLNRDINLPQIDPNTPASEAIERLSSALGRELPDDFGQIVVFDSD
                     QLTEVQTTLRWFNSGAIVLGIVAVLLIAATLLVSPHRRRTVISLGVSIAVVTALLRRT
                     TFISQKQVVEEVKPQNQAAANAVFDQVLSFYRAVTLWMLAICAVAVFVAWLVGPSRAA
                     VAIRGGVSRTGHRTADAAGDVSWLHTYASAARYGVLIVAGLIVLFGSFTFLSLVLFGL
                     VIAAIVIWLTWVGQSHDGDAPGTKSPDLTTEGRA"
     gene            1667..2242
                     /locus_tag="VFX21_03315"
     CDS             1667..2242
                     /locus_tag="VFX21_03315"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEX5095009.1"
                     /translation="MTTAAVTPSDEYEVVVEEGAGKYWWVLLVTGGLWLTFGWIVLSA
                     RSEITTVWAVCIYAGVLFLMFAAGELVGAFVVEGWRWLHVLVGLGALVAGVMCFVWPE
                     QTFITLAAILAWYLLFDGVLHIGRSLASRHEVDIWWMFLILGVLEVAIAFWAIGYTGR
                     SVALLVIWVGAMALAKGLAQIVGAFMLRSAR"
     gene            complement(2255..3070)
                     /locus_tag="VFX21_03320"
     CDS             complement(2255..3070)
                     /locus_tag="VFX21_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HEX5095010.1"
                     /translation="MPMFLDRHEGVDATPEEVAQAHTMDLAVEGKYGVHYHTYWFDTD
                     NRTIFCLAEGESKEAIDRVHKEAHGLSASTIIEIDPNVPLNQLFGSIVIAPAGTPTSE
                     SAVRGIVFTDLCGSVAQTQQLGDDGHVAMLREHDEIVRKALGAHQGREVKHTGDGIMA
                     AFTSVTAAVACAIDVQRALHQRNQIELIPLDVSIGISAGEPVTNDNDDLFGAAVQIAA
                     RLCGAAEAGEIMVSVAVRELCIGKQIGFADQGRIALKGLPEPAQVFGVVWRDG"
     gene            complement(3153..>3436)
                     /locus_tag="VFX21_03325"
     CDS             complement(3153..>3436)
                     /locus_tag="VFX21_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095011.1"
                     /translation="PLSDEVHANEHVRHFLGRVSLLWAFFQLANATLTIWLLLTQSPS
                     VFVVTRMAVSWTLTITAIVVSALWFRRSMARQGIVVTLPTWQRTGSAAV"
BASE COUNT          525 a         1125 c         1165 g          621 t
ORIGIN      
        1 ggtcaggcgt ggtcatgggc ggggagagcg gcacagcggc gtacgtctac cagctcgagg
       61 ccgagcgctt ctatccggcg cagcaaaccg tggagcgccg cctggtcgtc gacggtcccg
      121 gcgagcacgg tctcgaccgg cgcgatgctg gtcgtcaacg cgagctgctc gaactcggtc
      181 gcgagcgacg gcgtgagccg ccctcgcacc cgaatctcgt actggtgtgc gcccaatatc
      241 tcgacctcgg gctccatgat ctgctcatcc gaagcatggt gcgcggcccg cggccggcgt
      301 atcacccggc ctgggtgacg cgtgcgcgga ccgcacttca tcccatcggt gtcatgccgc
      361 acctgcggat tgtgcttagc ctgccgtcca tggcggcacc tgacgtacga gtcaactccc
      421 ggcgcgttcc ccgcgtcgtc atgggattca tggtcgtggt cacagccctg gtggtcggga
      481 tctcggccac cggcctgtgg atgcgcggcg tcgtgttccg cgagagccgc tggaagcaga
      541 cgatcgctcc gctcggctcc gacgacgaag tgcaggccgc gctcgcggcc tgggccggca
      601 ccgagatccg ccaggtcatc gacgtcaagc cgtacctcga agacatcctt cccagcggcg
      661 cgcaaggact cgcgtcgccg ttgtcgttcg cgatcgaagg gttcatcgcg gaacgcgccg
      721 cttcgttctt cgagagtgac cagttctccg aactgtggga acggtcgacc gtgcgactgc
      781 actccgcggt gttgaaagtg ctgagaggcg acagcgaagc agtctcggtc cgcgacggtc
      841 aggtcgtgct caatctcgtg ccgatcctga accaagtgct cgcgtcgatc tccgacgagc
      901 tttcacaact gctgaaccgc gacatcaacc tgccgcagat cgatccgaac acacccgcgt
      961 ccgaggcgat cgaacgcctg tcgagcgcac tcggacgcga gctgcccgac gacttcggtc
     1021 agatcgtcgt gttcgactcc gaccagctca cggaggtgca gacgacgctg cgttggttca
     1081 actcgggcgc catcgtcctc gggatcgtcg ccgtcttgtt gatcgccgcg acgctgctcg
     1141 tctcaccgca tcgtcgacga acggtcatct cgctcggagt atcgatcgcg gtggtcaccg
     1201 cactgttgcg gcgcacgacg ttcatctccc agaagcaagt cgtcgaagag gtgaagccgc
     1261 agaaccaggc cgcggccaac gccgtcttcg accaggtgtt gagcttctac cgcgccgtga
     1321 ccctctggat gctcgcgatc tgcgccgtcg cggtgttcgt cgcgtggctg gttgggccgt
     1381 cgcgggccgc cgtcgcgatt cgcggcggcg tgagccgcac ggggcaccgg accgcggacg
     1441 ccgcgggcga cgtctcgtgg ctgcacacgt acgcgagcgc ggcgcgctac ggcgtattga
     1501 tcgtcgccgg cctcatcgtg ctcttcggaa gcttcacgtt cctcagcctc gtcctgttcg
     1561 gattggtcat tgccgcgatc gtcatctggc taacgtgggt cggccagtca cacgacggtg
     1621 acgcacccgg cacgaagagt cccgacctga cgaccgaagg gcgcgcatga ccactgccgc
     1681 agtcacaccg agcgacgagt acgaagtcgt cgtcgaggag ggcgccggta agtactggtg
     1741 ggtactgctc gtcaccggtg gtctctggct gacattcggc tggatcgtgt tgagcgcgcg
     1801 cagcgagatc acgaccgtgt gggcggtctg tatctacgcg ggcgtcctct tcctgatgtt
     1861 cgcggcgggc gagctcgtcg gcgcgttcgt cgtcgagggg tggcggtggt tgcacgtgct
     1921 ggtcggcctc ggcgcgctcg tcgcgggcgt catgtgcttc gtctggcccg aacagacctt
     1981 catcaccctc gcggcgatcc tcgcgtggta cttgctgttc gacggcgtcc tgcacatcgg
     2041 gcgctcgctg gcgtcgcgcc acgaggtcga catctggtgg atgttcctga tcctcggcgt
     2101 gctcgaagtt gcgatcgcgt tctgggcgat cggctacacc ggacgctcgg tcgcgctgct
     2161 cgtcatctgg gtcggcgcaa tggccctcgc gaaggggctc gcgcagatcg tcggcgcgtt
     2221 catgctgcgc agcgcgcgct gacgcggatc gcggctaccc gtcgcgccag acgacgccga
     2281 agacctgcgc gggctcgggc aggcccttga gcgcgatccg gccctggtcg gcgaagccga
     2341 tctgcttgcc gatgcacagc tcgcgtacgg cgaccgacac catgatctcg ccggcttcgg
     2401 ccgcgccgca gagtcgcgcc gcgatttgca cggctgcgcc gaacaggtcg tcgttgtcgt
     2461 tggtgacagg ctcgccggcg ctgatgccga tgctcacgtc gagcgggatc aactcgatct
     2521 gattgcgttg gtgcagcgcc cgctgcacgt cgatcgcgca cgcgaccgcg gcggtcaccg
     2581 atgtgaacgc ggccatgatg ccgtcgccgg tgtgcttcac ctcgcgaccc tggtgggcac
     2641 ccagggcctt gcgcacgatc tcgtcgtgct cgcgcagcat cgcgacatgg ccgtcgtcgc
     2701 cgagttgctg cgtctgcgcg accgagccgc agagatcggt gaacacgatg ccgcgcacgg
     2761 ccgactcgga ggtcggtgtg ccggccggcg cgatgacgat cgagccgaac agctgattga
     2821 gtgggacgtt gggatcgatc tcgatgatcg tcgacgcgct cagaccgtgc gcttccttgt
     2881 gcacacgatc gatcgcctct ttgctctcac cttccgcgag acagaagatc gtgcggttgt
     2941 cggtgtcgaa ccagtacgtg tggtagtgca cgccgtactt gccttcgacc gcgaggtcca
     3001 tcgtgtgcgc ttgcgccacc tcttccggcg tggcatcgac accttcgtgg cggtcgagga
     3061 acatcggcat gacgtgagga tacagcggaa cgtggagccc ggcgccggaa gaatgcccgg
     3121 aagagcgggg ctctaccggg gctcgaccgc ggctataccg ccgcggagcc cgtgcgctgc
     3181 caggtcggca acgtcacgac gatgccctgg cgcgccatcg aacggcggaa ccacaacgcg
     3241 gagacgacga tcgcggtgat ggtgagcgtc cacgagacgg ccatgcgcgt gacgacgaag
     3301 acgctcggcg actgggtcag caacagccag atggtgagcg tcgcgttcgc gagctgaaag
     3361 aaggcccaca gcaaggagac gcgccccaag aagtggcgca cgtgttcgtt ggcgtgcact
     3421 tcgtcggaga gcgggc
//
LOCUS       DASUGA010000172         3120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1966500, whole genome shotgun sequence.
ACCESSION   DASUGA010000172 DASUGA010000000
VERSION     DASUGA010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1966500"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VFX21_03330"
     CDS             <1..448
                     /locus_tag="VFX21_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004938421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5095012.1"
                     /translation="IDRSPDATRQLASRARRRVRDERARPDPDVGRQREVVDAFYAAA
                     RAGNLDALVAVLDPDVVLRSDSGTGHVLELHGAEAVASRARAFSRSGLVHHPVLVNGA
                     AGTVSTRNGRPFSVASFTVRDGKIVELDFLADGARLAGLDLVVVGR"
     gene            complement(453..1232)
                     /locus_tag="VFX21_03335"
     CDS             complement(453..1232)
                     /locus_tag="VFX21_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5095013.1"
                     /translation="MEYEQILREQRDDVVVVTLNRPDKMNAWTPRMTAELTHAIEAAD
                     ADPSVGAVVMTGAGRGFCAGADISAVFEAQLEGDASASVPQGDRDWVDLIRSTKPIVA
                     AVNGPAIGMGLTMILPFDRIVVADGAKLSIRFVKLGLVPELASSMFLPLRCGWGAAND
                     LMLSGRTILPDEARALGVIDEVVAPGAVLDVAIRRAREYGENPTPQVRWIKQLLTQNA
                     NETDPRAVQMREVRRVAEAYATPEHKEAVAAFLEGRKPTFR"
     gene            complement(1349..1936)
                     /locus_tag="VFX21_03340"
     CDS             complement(1349..1936)
                     /locus_tag="VFX21_03340"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5095014.1"
                     /translation="MPAPARRPGKVAIMRAAVEVMAEEGFEGASTRDMAARAGVSVAA
                     LYHHFPSKLGLLQEFLDEAYDVTLARIERRLDGVEGPVARLENVVATLAWTHLHDDFA
                     RQASIVAVREYTRLDVPERAAIEVKRKALLDLVESIVKEGVRTKAFRTPDTRETARAI
                     VSLVQSLVGTFAEIDRGLDDVTALYQVLAVRLAAG"
     gene            1980..>3120
                     /locus_tag="VFX21_03345"
     CDS             1980..>3120
                     /locus_tag="VFX21_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX5095015.1"
                     /translation="MAEDQMGDLFAIDVVDIRGVPTKVFRNAPPSLRAIWDLSALFAA
                     NTYLVNGDERVTYAEAHRDVRAVVRWMLEQGVQPGDRVAIATRNHPEWVTAFWAAVAI
                     GAVVVPLNAWWTGPELAYGLADSGTTLLFADDERAERVAPHLSETNVRATVLIRTARD
                     LPGGVAWTYVVAGDDPALPALEVDSDADTAIMYTSGTTGRPKGAVQTQRNFTNFLMQG
                     AYFTTKAAASAPAAPGPPVPMATLLTLPLFHVGGLHSFLLPYTAAGGKVVLMYKWNAA
                     EAVDIVEREGITAIAGVPTTMFELLDAARAKGATLESLTGVSSGATLVPPELVRRIDT
                     QLESRAAPGNGYGLTETSGAAIANFGPAYVANPESVGKPISPVIEV"
BASE COUNT          470 a         1073 c         1077 g          500 t
ORIGIN      
        1 catcgaccgc tcaccggacg cgacgcgtca gctcgcgagt cgagcccggc gtcgagtgag
       61 ggacgaacgc gccaggcccg accccgatgt cggtcgccaa cgcgaggtcg tggacgcgtt
      121 ctacgccgcg gcgcgcgcgg gcaacctgga tgcgctcgtc gccgtcctcg accccgacgt
      181 cgtgctgcgc tccgactcgg gcacggggca cgtgctcgag ttgcacggag ccgaagcagt
      241 tgcttcgcga gcgcgcgcgt tttcccggtc cgggctggtc catcacccgg tgctggtcaa
      301 cggtgccgcc ggcaccgtgt cgacccgcaa cggccggccg ttctccgtcg cttcgttcac
      361 ggtgcgcgac ggcaagatcg tcgagctcga tttcctggcc gacggggcgc gcctcgccgg
      421 gctcgatctc gtcgtggtcg gtcgctgacg cgctagcgga atgtcggctt gcgcccttcg
      481 aggaacgcgg cgacggcttc cttgtgctcg ggcgtcgcgt acgcctcggc gacgcgccgg
      541 acctcacgca tctggacagc gcgcggatcg gtctcgttcg cgttctgcgt gaggagttgc
      601 ttgatccagc gcacctgcgg cgtcggattc tcgccgtatt cgcgtgcgcg tcggatggcg
      661 acgtcgagca ctgctcccgg cgcgacgacc tcgtcgatca cgccgagcgc gcgcgcttca
      721 tcgggaagga tcgtgcggcc cgacaacatg agatcgttcg ccgcgcccca tccgcagcgc
      781 agcggcaaga acatgctgct tgcgagctcg ggcacgagac ccagcttcac gaagcggatg
      841 ctgagcttgg cgccgtcggc gacgacgatg cggtcgaacg gcaggatcat cgtcaacccc
      901 ataccgatcg cgggcccgtt caccgcggcg acgatcggtt tggtcgaacg gatgaggtcg
      961 acccaatcgc gatcgccttg cggtaccgac gcggaggcgt cgccttcgag ttgcgcctcg
     1021 aacaccgcgc tgatgtcggc gcccgcgcag aacccgcgtc ccgcgccggt catcaccacc
     1081 gcgcccacgc tcgggtcggc gtcggcggct tcgatcgcgt gggtgagctc tgcggtcatg
     1141 cgcggcgtcc acgcgttcat cttgtcgggc cggttgagcg tgacgaccac gacgtcgtcg
     1201 cgctgctcac gcaggatctg ctcgtactcc acgggatgca cactaggccg cgtactggcg
     1261 caggaaccga gcgggtcgag cggcgacgac tgggtctcac tcgcaatggt ggtgcgcgac
     1321 aagctcgcgg tcgcgaaccc gaagcacctc agcctgccgc gagcctgacg gcgagcactt
     1381 ggtacagcgc ggtcacgtcg tcgaggccgc ggtcgatctc ggcgaaggtg ccgaccagcg
     1441 actggacgag gctgacgatc gcgcgcgcgg tctcgcgcgt gtcgggcgtg cggaaggcct
     1501 ttgtacgcac accttccttg acgatgctct ctacgaggtc gagcagcgcc ttgcgcttca
     1561 cttcgatcgc ggcgcgctcg ggaacgtcga gacgtgtgta ctcgcgcacc gcgacgatcg
     1621 atgcttgccg cgcgaagtcg tcgtgcaagt gtgtccatgc caaggtggcg acgacgttct
     1681 cgaggcgggc aacggggccc tcgacaccgt cgagccgccg ctcgatgcgg gcgagcgtca
     1741 cgtcgtacgc ctcgtcgagg aactcctgga gcagcccgag cttcgagggg aagtggtggt
     1801 agagcgcggc aacggacaca cccgcgcggg cggccatgtc gcgcgtcgac gcgccttcga
     1861 agccctcctc ggccatgacc tcgaccgcgg cgcgcatgat cgcgaccttg ccgggccggc
     1921 gggcgggagc gggcacggga ggagtgtacc gaaaaccgaa cgaacgctaa gttcgtgcca
     1981 tggccgagga ccagatgggg gacctgttcg cgatcgacgt cgtcgacatc cgcggcgtcc
     2041 cgacgaaggt cttccggaac gcaccgccgt cgttgcgggc gatctgggac ctgagcgcgc
     2101 tgtttgccgc gaacacgtac ctcgtgaacg gcgacgaacg cgtgacgtac gccgaggcgc
     2161 accgcgatgt gcgcgcggtc gtgcggtgga tgctcgaaca gggtgtgcag ccgggcgacc
     2221 gcgtcgccat cgcgacgcgc aaccatcccg agtgggtcac cgcgttctgg gccgcggttg
     2281 cgatcggcgc ggtcgtggtg ccgctgaacg cgtggtggac cggtcccgag ctcgcgtacg
     2341 gacttgccga ctccggcacg acgctgctgt tcgcggacga cgaacgcgcc gaacgcgtcg
     2401 caccgcacct cagcgagacg aacgtgcgcg ccaccgtctt gatccgcact gcgcgtgatc
     2461 tccccggtgg tgtcgcgtgg acctacgtcg tcgcgggtga cgatcccgcg ttgcccgcgc
     2521 tcgaggtcga ctccgatgcc gatactgcga tcatgtacac gtcgggcacg accggacgcc
     2581 ccaaaggcgc ggtgcagacg cagcgcaact tcacgaactt cttgatgcaa ggcgcgtact
     2641 tcacgaccaa ggctgctgcg tccgcgccgg ccgcacccgg accgccggtg ccgatggcga
     2701 cgctgctcac gttgcccttg ttccacgtgg gcggcctgca ttccttcctc ctgccctata
     2761 ccgcagcggg cggcaaggtc gtcctcatgt acaagtggaa cgcggccgaa gcggtcgaca
     2821 tcgtcgaacg tgaaggcatc actgcgatcg cgggcgtgcc gacgacgatg ttcgagctgc
     2881 tcgacgcggc ccgcgccaaa ggtgcgacgc tcgagtcgct cacgggcgtg tcgtcgggcg
     2941 cgacgctcgt gccgcccgag ctcgtccgcc gcatcgacac ccagctcgaa tcgcgcgccg
     3001 cgccgggcaa cggctacgga ctcaccgaga catcgggcgc cgcgatcgcg aacttcggtc
     3061 cggcgtatgt cgcgaacccc gagagtgtcg gcaaaccgat ctcgcccgtg atcgaagtgc
//
LOCUS       DASUGA010000173         9738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_472869, whole genome shotgun sequence.
ACCESSION   DASUGA010000173 DASUGA010000000
VERSION     DASUGA010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 9738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9738
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472869"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VFX21_03350"
     CDS             <1..803
                     /locus_tag="VFX21_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020461432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX5095016.1"
                     /translation="DPRLYACPSWYGELGQFITTKFFGMKINKYMTDHGISHETLAKV
                     AAKNYRNGALNENAFRRKPLSEEEILGSPYLNYPLTQYMFCSPDEGAAAVVLCKAEHA
                     TRYTGTPIYLRASTVRTRRLGAFEVHSPWLPIEETVGATVDASRAAYETAGVGPDDVD
                     VIQLQDTDAGAEVIHLAENGFCADGEQEALIAEGATEINGGLPVNTDGGLIANGEPIG
                     ASGLRQVHEVVLQLRGTAGDRQVPGNPTVGYTQLYGAPGTAGVSILTK"
     gene            803..1813
                     /locus_tag="VFX21_03355"
     CDS             803..1813
                     /locus_tag="VFX21_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5095017.1"
                     /translation="MDTRDTAEQAELRRAARQLAHDLGPATVRDLDDQTRRQRLAGAV
                     RDAGWLELRDDAGNGSPLASGVEACIVAEALGAAVADVPFSGPILAADLARRAGVTLV
                     PGAVVAFGADLVGTGEYPAFAVDAYGDVDAVHVVVRNDEQLQVGVTNAGAIERATDLT
                     RVVRAIPPGSFVADITGHLDADDVARWTALGLALTSADLVGVMRGAIDVTVAYAAERK
                     QYGVAIGSFQAVQHLLAEAAALCEGAVSVALHASWAVDDLAPADALTAGRVAKAYCAR
                     AARTVCETAIQVHGGIGNTWDCIAHVYLRRALLSARWFGDDAVQLRALERDLVGVADG
                     FS"
     gene            1800..2933
                     /locus_tag="VFX21_03360"
     CDS             1800..2933
                     /locus_tag="VFX21_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5095018.1"
                     /translation="MDFRDSPAEAEFRARFRAWLATNNPGLPTSSTDDEYWARAAEWH
                     TALYEAGFFGPSWPARYGGHDLPTVFDVILDDELAAAGAPPRPSLGYLVQGISTHGSE
                     EIKDRFLPGLISGRDRWCQGFSEPDAGSDLASLRTTATRDGDEFVIDGHKIWTSYSDV
                     ADWCFLLARTDPDVPKHKGLTAFAISMDQPGIEQRPLPMINGITKEFGQVLFDGARVP
                     AANMIGQPGEGWRVAMTIVSHEREPGELGYVARYAKTVKELRHRVKTNPNGYHRDQRE
                     AVAWAHVQTEMLRLHVCRRLSERLDGIEHGPGGSIDKLLMTWVEQTVGTAALDVSGAD
                     AVVDGDDVALKTYLYSRAQSVMGGTSQIQKNIIATRILGLPTS"
     gene            2944..3714
                     /locus_tag="VFX21_03365"
     CDS             2944..3714
                     /locus_tag="VFX21_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007777529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5095019.1"
                     /translation="MTYDLPDVIRVESDGPVRIVRLSRPEQLNAINPELHLGLTRVFP
                     QLSEDHDARVAVITGEGRAFSAGGDFELLDNMVKDRALRQETIAEGRELVLNMLRCRV
                     PVVAAVNGPAVGLGCSVIALSDVVYMAESAYLSDPHVNVGLVAADGGPLTWPLHTSLL
                     LAKEYAFTGDRISAIRAAEIGLANHVCADGEVLHEALTAAHKIAKLPQQAVEATKRVL
                     NLHAERAVLATIDFAMSAETASFDTPELRANVDRFLGR"
     gene            3714..5345
                     /locus_tag="VFX21_03370"
     CDS             3714..5345
                     /locus_tag="VFX21_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018637478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX5095020.1"
                     /translation="MRLLPQLLPANEWYWTSGADGHLRIQRCVDCATYVHPPVPICPA
                     CRSLKWEPTIVSGRGTVVGFTVNAHQWLPGFDPPYVIANVALTEDPSVRLTTNIVDCA
                     PDDVAIGAEVEVRFEQHDDVWIPLFALTGAMDPVDRVPEPQRPTTRPPARTDRFEHRV
                     VLSGIGRSAIGRRLMRDPLSLTVDACLAAVADAGLTLDDIDGLSTYPGATGGLTGISE
                     GGVTAVEEALRIRPTWTNGGMELPGPGGSVIAAMLAVASGLCRHVLCFRTVWESTYAK
                     LGLGSGGRRVSGPLMEWRAPYGAMSAANWIGMNANQYLHRYRATRENTMGLIALNGRA
                     NAARNPLAIYREPMTMDDYLSARPITSPFGLYDCDVPCDASVAVIVSDAWVANDLPHP
                     AIRVDAVGTQITERISWEQDTITHEPQVLGQAAHLWTRTDLRPQDVDLALVYDGFTFN
                     AITWLEALGFCELGEAQDWLDGGKRIGLDGDLPVNPHGGQLSEGRTHGYGFFYEAVTQ
                     LRGDAGERQVQNAQTAVVTSGGGTPSGVLLLRRNG"
     gene            5342..6076
                     /locus_tag="VFX21_03375"
     CDS             5342..6076
                     /locus_tag="VFX21_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018501238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5095021.1"
                     /translation="MSRVAVVTGAGSGMGLAIARRLAADGHRVAMLDISGDAVGAAAA
                     EIGGNAMPIGVDVSNRPGIETALSLVRHQFGPIEIMVTSAGIDRFTSFTDITVDDWQQ
                     MIEVNLTGTFHCVQAAVPDMLATGWGRIVTISSSSAQSGSARMAHYVASKGGVVGLTK
                     ALALELAPKGITVNTIPPGFIDTPMARNAEARGDLPSFDAVIARTPVRRAGTPEDVAA
                     ACAFLCSDEASYITGQAINVNGGWYL"
     gene            6073..6633
                     /locus_tag="VFX21_03380"
     CDS             6073..6633
                     /locus_tag="VFX21_03380"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5095022.1"
                     /translation="MTEPRIPPLPVEQWDDGARAALDGVFPANVVDAWVAGAAPVPNV
                     VSTMLQHPKLAGPWLAYNNVLLWSPTLTDRRRELLVLRVARKANSAYEWAQHVKLADR
                     FGITAADIDAIEHDRANDAWTPFERALIDSTDQLVDTHCIADDTWAQLAQELDQRQLV
                     ELVFVVGTYTCLAMAFNSFGMQLDES"
     gene            complement(6630..7823)
                     /locus_tag="VFX21_03385"
     CDS             complement(6630..7823)
                     /locus_tag="VFX21_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020461551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5095023.1"
                     /translation="MTGQVADVYYDPYDFDIDADPYPVWKRLRDEAPLYYNERYDFYA
                     LSRFDDVERASVDWKTYISSKGTILELIKSGFEVPPGSIIFEDPPTHDIHRSLLSRVF
                     TPRKVSALEPKIREFCARSLDPLVGSGGFDFIADLGAQMPMRTIGMLLGIPEQDQEAI
                     RDFGDERLRLEDGTMPEIDVPDFGSDNIFGDYLDWRLEHPSDDLMTELLNAEFDDAEG
                     VRRKLTREEVLGYILLLAGAGNETTTRLIGWTGKLLAEHPDQRAALAADRSLIPNAIE
                     EILRYESPSPVQARYVTADVEHYGQKVPEGSVMLALTASGNRDERKFADADRFDIHRK
                     IDHHLAFGYGIHFCMGAALARMEGRVALDEVLQRFPEWEVDWENAAQARTSTVRGWEK
                     LPVRV"
     gene            7912..9192
                     /locus_tag="VFX21_03390"
     CDS             7912..9192
                     /locus_tag="VFX21_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX5095024.1"
                     /translation="MPHFTKPDEGSWTGHYPELGTAPVSYEDSISPEIYDLERDAIFG
                     RTWLNVGRVEQLAKKGSYFTKQLDAARTSVIVVRGMDDEIRAFHNMCRHRGNKLVWTD
                     FPLEEVSGTCRNFTCKYHGWRYDLTGALTFVQQEEEFFDLDKSDYPLASVQCDVWEGF
                     IFVNLESGNSTSLHEYMGELGKGLEGYPFHEFTQVHKFRAEVGANWKLFIDAFTEFYH
                     APVLHAKQAVADESTKLQGYGYEALAYGIDGPHGMVSSWGGMSPPKDLNMVKPIERVL
                     RSGLFGPWDQPDIDGLDELPPGINPARHKAWGTDSFLFFPNFMILIWKPGWVLTYHYW
                     PTSYNTHVFEGTCYFAPPKTARERLAQELAAVTFKEYALQDGNTLEATQTMLESRTPI
                     TRFPLNDQEILIRHLHHTTKQYITDYQRQQGANR"
     gene            9189..9521
                     /locus_tag="VFX21_03395"
     CDS             9189..9521
                     /locus_tag="VFX21_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005191142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095025.1"
                     /translation="MSVATLPAEFADLTPFAEFARPTAKERYEKRISCTMPELQAFYD
                     AAFPRFEDALAYLDRFDLDALPDDAKQLMYLYCALVTVSFPVEVWRQPRVPDSGAAAF
                     DEISEPTI"
     gene            9526..>9738
                     /locus_tag="VFX21_03400"
     CDS             9526..>9738
                     /locus_tag="VFX21_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5095026.1"
                     /translation="MPVTDAEAADEQQPAWRQRAVSKSLHAARSRAEQRAQRFIDAAF
                     ELIDEKGTTDFTIQEVIDRSKQSLRGF"
BASE COUNT         1625 a         3374 c         3246 g         1493 t
ORIGIN      
        1 ccgacccgcg cctctacgcg tgcccgtcgt ggtacggcga gctcgggcag ttcatcacga
       61 ccaagttctt cggcatgaag atcaacaagt acatgaccga ccacggcatc tcgcacgaga
      121 cgctggccaa ggtcgcggcg aagaactacc gcaacggcgc gctgaacgag aacgccttcc
      181 gccgcaagcc gctgtcggaa gaggagatcc tcgggtcgcc gtatctcaac tacccgttga
      241 cgcagtacat gttctgcagc ccggatgaag gtgcggccgc ggttgttttg tgcaaggccg
      301 aacacgcgac gcgctacacc ggcacgccga tctacctgcg cgcctccacc gtgcgcacgc
      361 gtcgcctcgg tgcgttcgaa gtgcacagtc cgtggctccc aatcgaggag actgtcggcg
      421 cgaccgtcga cgcatcgcgc gccgcctacg agaccgcggg tgtcggtccc gacgacgtcg
      481 acgtgatcca gctccaggac accgatgcgg gcgccgaggt cattcacctc gcggagaacg
      541 ggttctgcgc ggacggtgag caagaagcac tcattgccga aggtgcgacc gagatcaacg
      601 gtggcctgcc ggtgaacacc gacggcggcc tcatcgccaa cggcgaaccg atcggcgcgt
      661 ccgggttgcg gcaggtgcac gaggtcgtgt tgcagctgcg cggcaccgcc ggcgaccggc
      721 aggtgccggg caacccgacc gtcggttaca cgcagctcta cggcgcgccc ggcaccgccg
      781 gcgtctcgat cctcacgaag taatggatac gagggacacg gccgaacagg ccgagctccg
      841 gcgcgcggcg cgccaactcg cgcacgacct cggtcccgca actgtgcgcg acctcgacga
      901 ccagacgcgc cggcaacggc tcgcgggcgc cgtacgcgac gccggttggc tcgagctgcg
      961 cgacgacgcg gggaacggct cgccactcgc gagcggcgtc gaggcgtgca tcgtggccga
     1021 ggcgctcggc gccgcggtcg ccgatgtgcc gttcagcggt ccgatcctcg cggccgacct
     1081 cgcgcggcgc gccggtgtga cgctcgtacc gggtgccgtc gtcgcgttcg gcgccgatct
     1141 cgtcggcacg ggcgagtacc cggccttcgc ggtcgacgcg tacggtgacg tcgacgcggt
     1201 ccatgtcgtg gtccgcaacg acgagcagct ccaggtcggt gtcaccaacg ccggcgcgat
     1261 cgagcgggcg accgacctca cacgcgtcgt gcgggcgatc ccgcccggat cattcgtcgc
     1321 cgacatcacc ggtcatctcg acgcggacga cgtcgcccgg tggaccgccc tcgggctcgc
     1381 gctgaccagc gccgacctcg tcggtgtgat gcgcggtgcc atcgacgtca ccgtcgcgta
     1441 cgcggcggag cgcaagcagt acggcgtcgc gatcggttcg ttccaagccg tgcagcatct
     1501 cctcgcggaa gccgcggcgt tgtgcgaggg cgcggtgagc gtcgcgctgc atgcgtcgtg
     1561 ggcggtcgac gatctcgcgc ccgccgacgc cctcaccgcg ggccgcgtcg cgaaggcgta
     1621 ctgcgcccgc gccgcgcgca cggtgtgcga aaccgcgatc caagtgcacg gcggcatcgg
     1681 caacacctgg gactgcatcg cgcatgtcta cctgcggcgc gccttgctgt cggcgcggtg
     1741 gttcggtgac gatgcggtac agctgcgcgc gctcgagcgg gacctggtgg gagtcgccga
     1801 tggattttcg tgactccccc gccgaggccg agttccgcgc gcggttccgc gcgtggctcg
     1861 cgaccaacaa tccgggcttg ccgacgtcgt ccaccgacga cgagtactgg gcgcgcgcgg
     1921 ccgagtggca caccgcgctg tacgaggcgg gcttcttcgg gccgagctgg cccgcgcgct
     1981 acggcggcca cgacctgccg acggtgttcg acgtcatcct cgacgacgag ctcgcggccg
     2041 cgggcgcgcc gccccgcccg agcctcggct atctcgtgca aggcatctcg acccacggca
     2101 gcgaggagat caaggaccgc ttccttcccg ggctcatcag cggccgtgac cgttggtgcc
     2161 agggcttcag cgaacccgac gccggctccg acctggcgtc gttgcgcacg accgcgacgc
     2221 gcgacggcga cgagttcgtg atcgacgggc acaagatctg gacgagctac tccgacgtcg
     2281 ccgactggtg tttcctgctc gcgcgcaccg atcccgacgt cccgaagcac aagggcttga
     2341 ccgcgttcgc gatctcgatg gaccagcccg gcatcgagca acgaccgttg ccgatgatca
     2401 acggcatcac caaggagttc ggccaggtgt tgttcgacgg cgcgcgggtg cccgccgcca
     2461 acatgatcgg gcaaccgggc gaaggctggc gcgtcgcgat gacgatcgtc agccacgagc
     2521 gcgagccggg cgagctcggc tacgtcgcgc gctacgcaaa gacggtcaag gagctccggc
     2581 accgcgtcaa gacgaatccg aacggctacc accgcgacca gcgcgaggcc gtggcgtggg
     2641 cgcacgtgca aaccgagatg ctccgcctcc acgtctgccg gcgcctctcc gagcggctcg
     2701 acggcatcga gcacggtccc ggcggctcga tcgacaagct gctcatgaca tgggtcgagc
     2761 agaccgtggg caccgcggcg ctcgacgtgt ccggcgccga cgcagtcgtc gacggagacg
     2821 acgtcgcgct caagacgtac ctctacagcc gcgcccagag cgtcatgggt ggcacgtcac
     2881 agatccagaa gaacatcatc gcgacgcgca tcctcggcct cccgaccagt tgaaaggttc
     2941 ttcgtgacct acgacctgcc cgacgtcatc cgcgtcgagt ccgacgggcc cgtccgcatc
     3001 gtgcggctgt cgcgtcccga gcagctgaac gcgatcaacc ccgagctgca cctcggcctc
     3061 acgagggtgt tcccgcagct gagcgaggat cacgacgcgc gcgtcgccgt gatcaccggc
     3121 gagggccgcg cgttctccgc gggtggtgac ttcgagctcc tcgacaacat ggtgaaggac
     3181 cgcgcgctac ggcaggagac gatcgccgag gggcgcgagc tggtgctgaa catgctgcgc
     3241 tgccgggtgc cggtcgtggc cgccgtcaac ggtcctgcgg tcgggctcgg gtgcagcgtg
     3301 atcgctctct ccgacgtcgt ctacatggcc gagtcggcgt acctctccga cccacacgtc
     3361 aacgtcgggc tcgtcgccgc cgacggcgga ccgctcacgt ggccgttgca caccagcttg
     3421 ctgctggcca aggagtacgc cttcaccggt gatcgcatca gcgccatccg cgcggccgag
     3481 atcggactcg ccaatcatgt gtgcgcagac ggcgaggtgc tgcacgaagc gttgaccgcc
     3541 gcgcacaaga tcgcgaagtt gccccagcag gccgtcgagg cgacgaagcg ggtcttgaac
     3601 ctgcacgccg agcgcgcggt gctcgcgacg atcgacttcg cgatgtccgc ggagaccgcg
     3661 tcgttcgaca cgccggagct gcgcgccaac gtcgaccgct tcctcggccg ctgatgcgcc
     3721 tactccccca gctgctcccg gccaacgagt ggtactggac gtcgggcgcc gacggccacc
     3781 ttcgcatcca gcgctgcgtc gactgcgcga cgtacgttca tccgccggtg ccgatctgcc
     3841 cggcctgccg cagcctgaag tgggaaccga cgatcgtgtc ggggcgtggc accgtcgtcg
     3901 ggttcaccgt caatgcccac caatggctcc cgggcttcga tccgccctac gtcatcgcga
     3961 acgtcgcact gaccgaggac ccgagcgtcc ggctgacgac gaacatcgtc gactgcgcgc
     4021 ccgacgacgt cgcgatcggc gccgaagtcg aagtgcgctt cgagcagcac gacgacgtct
     4081 ggatcccgtt gttcgcgctc accggcgcga tggatcccgt cgatcgcgtg cccgaaccac
     4141 aacggccgac gacgcgcccg ccggctcgca ccgatcgctt cgagcaccgg gtcgtgctct
     4201 cgggcatcgg ccgctccgcg atcgggaggc ggttgatgcg cgacccgctc tcgttgaccg
     4261 tcgacgcgtg tctcgcggca gtcgccgacg cgggcctcac gctcgacgac atcgacggcc
     4321 tgtcgaccta tcccggtgcg acaggcgggt tgaccggcat cagcgaaggc ggtgtcaccg
     4381 cggtcgaaga agcgctgcgc atccgtccga cgtggaccaa cggcgggatg gagctgcccg
     4441 gaccgggcgg atcggtgatc gcggcgatgc tcgccgtcgc cagtggcctg tgccggcacg
     4501 tgttgtgctt ccgcaccgta tgggagtcga cgtatgccaa gctcgggctg ggttcgggcg
     4561 gccggcgcgt ctcgggcccg ctgatggagt ggcgcgcgcc gtacggcgcg atgtcggccg
     4621 cgaactggat cggcatgaac gccaaccagt acctgcaccg gtaccgcgcc acccgcgaga
     4681 acacgatggg gttgatcgca ctgaacgggc gcgccaacgc ggcccgcaac ccactcgcca
     4741 tctaccgcga gccgatgacg atggacgact acctgtcggc gcgcccgatc acgtcgccat
     4801 tcggcctcta cgactgcgac gtaccgtgcg acgcatccgt cgcggtcatc gtgtcggacg
     4861 cctgggtggc gaacgacctg ccgcatcccg cgatccgcgt cgacgcggtc ggcacgcaga
     4921 tcaccgagcg catctcgtgg gaacaggaca cgatcacaca cgaaccgcag gtgctcggcc
     4981 aggccgcgca cctgtggacg cgcaccgacc tgcggcccca agacgtcgat ctcgcactgg
     5041 tgtacgacgg cttcacgttc aacgcgatca cgtggctgga ggcgttgggc ttctgcgagc
     5101 tcggggaggc gcaggactgg ctcgacggcg gaaagcgcat cgggctcgac ggcgacctgc
     5161 cggtcaaccc gcacggcggc cagctgtcgg aaggcagaac gcacggttac ggtttcttct
     5221 acgaagccgt gacccagttg cgcggcgacg ccggcgaacg ccaagtgcag aacgcccaaa
     5281 ctgcggtggt gacatcaggc ggtggcacgc cctccggcgt gctcttgctc aggaggaacg
     5341 gatgagtcgg gtggcggtgg tcacgggagc ggggtccggc atggggctcg ccattgcgcg
     5401 gcggttggcc gccgacggac atcgagtcgc gatgctcgac atctccggcg acgcggtcgg
     5461 tgccgccgcc gcggagatcg gcggcaacgc gatgccgatc ggcgtcgacg tcagcaaccg
     5521 gcccggcatc gagaccgcgc tgtcattggt gcggcatcag ttcggcccga tcgagatcat
     5581 ggtgacgagc gccggcatcg accgcttcac gtcgttcacc gacatcacgg tcgacgactg
     5641 gcagcagatg atcgaggtca acctcaccgg cacgttccac tgcgtgcaag ccgcagtccc
     5701 cgacatgctc gcgacgggtt gggggcgcat cgtgacgatc tcgtcgtcga gcgcgcagtc
     5761 ggggtcggcg cgcatggcgc actacgtcgc gtcgaagggc ggcgtggtcg ggctgaccaa
     5821 ggcgctcgcg ctcgagctgg cgccgaaggg catcaccgtc aacacgatcc cgcccgggtt
     5881 catcgacacg ccgatggctc gcaatgccga agcgcgcggc gatctcccca gcttcgacgc
     5941 ggtgatcgcg cgcaccccgg ttcgccgcgc cggcaccccc gaagatgtcg cggccgcgtg
     6001 cgcgttcctc tgctccgacg aggcgagcta catcaccggg caggcgatca acgtcaacgg
     6061 tggctggtac ctgtgaccga gccgcgcatc ccgccgctcc ccgtcgagca gtgggacgac
     6121 ggcgcccgcg ccgcactcga cggcgtgttt cccgcgaacg tcgtcgacgc atgggtcgcg
     6181 ggcgcagcac ccgtgccgaa cgtcgtctcg accatgctcc agcacccgaa gctggcgggc
     6241 ccgtggctgg cgtacaacaa cgtgctgctg tggagcccga cgctcaccga ccgccggcgc
     6301 gagctgctgg tgctgcgcgt cgcccgcaag gcgaacagcg cgtacgaatg ggcgcaacac
     6361 gtcaagctcg ccgaccgttt cggcatcacc gcagccgaca tcgacgcgat cgagcacgac
     6421 cgcgcgaacg acgcgtggac gccgttcgaa cgcgcgctca tcgactcgac cgatcagctc
     6481 gtcgacacgc actgcattgc cgacgacaca tgggcgcagc tcgcgcaaga actcgaccaa
     6541 cggcaactcg tcgagctggt gttcgtcgtc gggacataca cgtgcctcgc gatggcgttc
     6601 aacagcttcg gcatgcagct cgacgagagc tagacgcgta cggggagctt ctcccacccg
     6661 cgcacggtcg acgtgcgcgc ctgcgccgcg ttctcccagt cgacctccca ctcgggaaac
     6721 cgctgcaaca cctcgtcgag cgcgacccgc ccctccatgc gggcgagcgc ggcgcccatg
     6781 cagaaatgga tgccgtagcc gaacgcgagg tgatggtcga tcttgcggtg gatgtcgaag
     6841 cggtcggcat cggcgaactt ccgctcgtcg cggttgcccg acgcggtgag ggccaacatc
     6901 acactgccct cgggcacctt ctgcccgtag tgctcgacgt ccgcggtgac gtagcgagcc
     6961 tgcaccggcg acggtgactc gtagcgcaag atctcctcga tcgcgttcgg gatcagcgac
     7021 cggtcggcgg cgagggcggc gcgctggtcg gggtgctcgg ccagcagctt gcccgtccaa
     7081 ccgatgaggc gcgtcgtggt ctcgttgccc gcaccggcca gcaagaggat gtagccgagc
     7141 acttcttcac gcgtcagctt ccggcgcaca ccttccgcgt cgtcgaactc cgcgttcagc
     7201 aactcggtca tgaggtcgtc ggagggatgc tccagccgcc agtcgagata gtcgccgaag
     7261 atgttgtcgg agccgaagtc gggcacgtcg atctccggca tcgtgccgtc ttcgaggcgc
     7321 agccgttcgt cgccgaagtc gcggatggct tcctggtctt gttcgggaat gccgagcagc
     7381 atgccgatcg tgcgcatcgg catctgcgcg ccgaggtccg cgatgaagtc gaatccaccc
     7441 gagccgacga gcgggtcgag gctgcgcgcg cagaactcgc ggatcttcgg ttcgagcgcg
     7501 ctcaccttgc gcggcgtgaa cacgcgcgac agcaacgagc gatggatgtc gtgcgtcggc
     7561 gggtcctcga agatgatcga gccgggcggc acctcgaagc cgctcttgat cagctcgagg
     7621 atcgtgccct tgctcgagat gtacgtcttc cagtcgaccg acgcgcgctc gacgtcgtcg
     7681 aagcggctca gcgcgtagaa gtcgtagcgc tcgttgtaat agaggggcgc ttcgtcgcgc
     7741 aggcgcttcc agaccgggta cggatcggcg tcgatgtcga agtcgtaggg gtcgtagtag
     7801 acgtcggcaa cctgcccggt catcggctga acccctgttc cctcgggtcg gaaacgccct
     7861 tctcgttttc ggagattgat gctatcgtaa cgccggcgaa cggaggatgc cttgccccat
     7921 ttcaccaagc ccgacgaggg cagctggacc gggcactacc ccgagctcgg caccgcgccg
     7981 gtgtcgtacg aagactcgat ctcgcccgag atctacgacc tcgaacgtga cgcgatcttc
     8041 gggcgcacgt ggctgaacgt gggccgcgtc gaacagctcg cgaagaaggg cagctacttc
     8101 accaagcagc tcgacgcggc gcgcacgtcg gtgatcgtgg tgcgcggcat ggacgacgag
     8161 atccgcgcgt tccacaacat gtgccgccac cgcggcaaca agctcgtgtg gaccgacttc
     8221 ccgctcgaag aagtgagcgg cacgtgccgc aacttcacgt gcaagtacca cgggtggcgc
     8281 tacgacctca cgggcgcact gaccttcgta cagcaagaag aagagttctt cgacctcgac
     8341 aagagcgact acccactcgc gtcggtgcag tgcgacgtgt gggaagggtt cattttcgtg
     8401 aacctcgaat ccggcaactc gacgtcgttg cacgagtaca tgggcgagct cgggaagggt
     8461 ctcgaggggt acccgttcca cgagttcacg caggtgcaca agttccgcgc cgaggtcggc
     8521 gccaactgga agttgttcat cgacgcgttc accgagttct accacgcacc cgtgttgcac
     8581 gcgaagcagg cggtcgccga cgaatcgacg aagctgcagg gttacggcta cgaagcgctg
     8641 gcgtacggca tcgacggtcc gcacggcatg gtgtcctcgt ggggcggcat gtcgccgccg
     8701 aaggacctca acatggtcaa gccgatcgag cgcgtcctgc gcagcggcct gttcggtccg
     8761 tgggatcagc ccgacatcga cggcctcgac gagctgccgc ccggcatcaa ccccgcccgg
     8821 cacaaggcgt ggggcaccga ttcgttcttg ttcttcccga acttcatgat cctgatctgg
     8881 aagccgggct gggtgctcac gtaccactac tggccgacgt cgtacaacac gcacgtcttc
     8941 gaaggcacgt gctacttcgc gccaccgaag accgcgcgcg aacggctcgc gcaggagctc
     9001 gcggcggtga cgttcaagga gtacgcgctc caggacggca acacgctgga agcgacgcag
     9061 accatgctcg aatcccgcac gccgatcacg cgcttcccgc tcaacgacca ggagatcttg
     9121 atccgccacc tgcaccacac cacgaagcag tacatcaccg actaccagcg gcagcagggg
     9181 gccaaccgat gagcgtcgcg acactgccgg ccgagttcgc cgacctcacg ccgttcgccg
     9241 agttcgcgcg gccgacggcc aaggaacgct acgagaagcg catctcgtgc accatgcccg
     9301 agctgcaggc gttctacgac gccgcgttcc cgcgcttcga ggacgccctc gcctatctcg
     9361 accgcttcga cctcgacgcg ctcccggacg acgcgaagca gctgatgtac ctgtactgcg
     9421 cgctcgtgac cgtgtcgttc ccggtcgagg tgtggcggca gccacgggtg cccgacagcg
     9481 gtgcagccgc gttcgacgag atctccgaac ctacaatctg accggatgcc cgtgaccgat
     9541 gccgaagccg ccgacgagca acaaccggcc tggcggcaac gcgcagtctc caagagtctg
     9601 catgcggccc ggtcccgcgc cgaacagcgc gcccaacggt tcatcgacgc cgcattcgag
     9661 ctgatcgacg agaagggcac gacggacttc acgatccagg aagtcatcga ccgctcgaag
     9721 cagtcgttgc gcggcttc
//
LOCUS       DASUGA010000174         2587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1452093, whole genome shotgun sequence.
ACCESSION   DASUGA010000174 DASUGA010000000
VERSION     DASUGA010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1452093"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFX21_03405"
     CDS             complement(<1..329)
                     /locus_tag="VFX21_03405"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004984376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX5095027.1"
                     /translation="MEVEIGIGKTGRRAYGFDDIAIVPSRRTRDPEDVDITWEVDAFK
                     LELPMMAAAMDGVVSPSTAVEIGRLGGLACLNLEGLWTRYVDPEPIFDEIASLPAEKA
                     TRRMQEL"
     gene            complement(426..1100)
                     /locus_tag="VFX21_03410"
     CDS             complement(426..1100)
                     /locus_tag="VFX21_03410"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX5095028.1"
                     /translation="MPPGGQQRIPRPATFRPGRPAPWTHLAPARRRLSLADVRERLSV
                     LPPGAGPVPLVDGSLPAAVLVPLYDAAGEATVILTKRPDTMPSHQGEISFPGGKFDPA
                     VDTDLEDTALREAQEEIGLPPGEVEVVARLEGIATVASRFLITPFVGFLSSRPALTPN
                     PGEVVRVLEVPLSDLMADDVYREEQWDTRGYDFDVHFYELSDETVWGATARVLTSFLA
                     HLVADR"
     gene            1160..>2587
                     /locus_tag="VFX21_03415"
     CDS             1160..>2587
                     /locus_tag="VFX21_03415"
                     /inference="COORDINATES: protein motif:HMM:NF005863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5095029.1"
                     /translation="MTWNLASVWESVADAVGDRVALVHGDQRTTWREFDDRSARLAAA
                     LREAGLGVDSKVASYLYNSNEYVEGVFATFKLRAIPVNVNYRYTEDELLYLLDNSDAE
                     AVLFHSSLGDRVAAVKDRAPLVKAWIEVDDGSEHQPFADRYEEVIASHDPMPRIERSE
                     DDMYFLYTGGTTGMPKGVMWRAGDLWGVLAGSTYALVGKQPPASLDDVGRVAKELADE
                     GRDIAHLPASPLMHGTGAFSSFQSMFLGGCIVTLTGRHFDAHELWQTVQRERVTQGAI
                     VGDAFAKPMLKALEEAEDRGEPYDLSSLILLISSGVMFSSEVKKGLMQRGQFICFDSL
                     GSSEGVGFANAITAPGGEQTTAKFTIGPEARVLKEDGVEVTPGSDEVGMLAVGGNIPV
                     GYYKDESKSAATFRVVNGKRWSIPGDFARVEEDGTIVLLGRGSVVINSGGEKIYPEEV
                     EEAVKLNPAVADCLVVGVPDDRFGEA"
BASE COUNT          423 a          873 c          895 g          396 t
ORIGIN      
        1 tacagctcct gcatgcgccg cgttgccttc tccgcgggca gcgatgcgat ctcgtcgaag
       61 atgggctcgg ggtcgacgta tcgcgtccac aggccttcga ggttgaggca cgcgaggccg
      121 ccgagccggc cgatctcgac cgcggtgctc ggcgagacga caccgtccat cgcggccgcc
      181 atcatcggca gctcgagctt gaacgcgtcg acttcccagg tgatgtcgac gtcctcggga
      241 tctcgcgtgc gccggctcgg gacgattgcg atgtcgtcga acccgtaggc ccggcgtccg
      301 gtctttccga tcccgatctc gacttccact ggcccctccg ggtctccgcg cggtggaaga
      361 accgaggata tcgccacccg ctgcccggac ggcgcaacgg cccgaaaacc cctgcgaaat
      421 gggccttagc ggtccgcgac gaggtgcgcg agaaagctcg tgagcacacg cgcggtcgcg
      481 ccccacacgg tctcgtcgct cagctcgtag aagtggacgt cgaagtcgta gccgcgtgtg
      541 tcccactgct cctcgcggta cacgtcgtcg gccatcaaat ccgacaacgg cacttcgagc
      601 acgcgcacga cttcgccggg attgggtgtg agcgcgggtc gactcgagag gaagccgacg
      661 aacggcgtga tcaagaaccg cgacgcgacg gtcgcgatcc cttcgaggcg cgccacgacc
      721 tccacctcgc ccggcgggag accgatctcc tcctgcgcct cgcgcagcgc ggtgtcctcg
      781 aggtcggtgt cgaccgcggg atcgaacttg ccgcccggaa acgagatctc gccctgatgc
      841 gacggcatcg tgtcaggccg cttggtgagg atcaccgtgg cttcgccggc cgcgtcgtac
      901 aacggcacca gcaccgccgc cggcaagctg ccgtcgacca gcggcaccgg ccccgcgccg
      961 ggcgggagca cggagaggcg ctcgcgcacg tcggccaacg agagccgccg tcgcgccggc
     1021 gcgaggtgcg tccagggcgc gggccggccc ggccggaacg tggccggccg gggaatgcgc
     1081 tgctgtcctc ccggcggcat gcggccaccg tagttgtgct gcgaaactgc cgactcatcc
     1141 actgcctcga ggagacacgt tgacctggaa cctcgcctcc gtctgggaat cggttgcgga
     1201 cgcggtgggc gatcgcgtcg cgctcgtcca cggcgaccag cgcacgacgt ggcgcgagtt
     1261 cgacgatcgg tcggcgcgcc ttgccgccgc gctgcgcgag gccggtctcg gtgtcgactc
     1321 gaaggtcgcg tcgtacctct acaacagcaa cgagtacgtc gaaggcgtct tcgccacgtt
     1381 caagctgcgc gcgatcccgg tcaacgtcaa ctaccgctac accgaggacg agcttctcta
     1441 tctgctcgac aactccgacg cggaggcggt gctgttccac agttcgctcg gcgaccgcgt
     1501 cgctgcggtg aaggaccgcg cgccgctcgt caaagcctgg atcgaagtcg acgacggcag
     1561 cgagcaccag ccgttcgccg acaggtacga agaagtgatc gcgtcgcacg atcccatgcc
     1621 gcggatcgag cgcagcgaag acgacatgta tttcctgtac accggcggca ccacgggcat
     1681 gcccaaaggt gtgatgtggc gagccggtga cctgtggggc gtgctggccg ggtcgaccta
     1741 cgccctggtg ggcaagcagc cgcccgcgtc gctcgacgac gtcggccgcg tcgccaagga
     1801 gctcgccgac gaaggccgcg acatcgcgca cttgccggcg tcgccgttga tgcacggcac
     1861 cggcgcgttc tcgagcttcc agtcgatgtt cctcgggggg tgcatcgtga cgctgaccgg
     1921 ccggcacttc gacgcgcacg agctctggca gacggtgcag cgtgagcgag tcacgcaggg
     1981 ggcgatcgtc ggcgacgcgt tcgcgaagcc gatgctgaag gcgctcgaag aggccgaaga
     2041 caggggtgag ccgtacgact tgtcgtcgct catcttgctc atcagcagcg gcgtgatgtt
     2101 ctcgtcggag gtcaagaagg gcctgatgca gcgcgggcaa ttcatctgct tcgactcgct
     2161 cgggtcgagc gagggcgtcg gcttcgcgaa cgcgatcacc gcgccgggcg gcgagcagac
     2221 gacggcgaag ttcaccatcg ggcccgaggc gcgggtgctg aaggaagacg gcgtcgaggt
     2281 cacgcccggc tccgacgagg tcggcatgct cgcggtcggc ggcaacatcc cggtcggcta
     2341 ctacaaggac gagtcgaaga gcgcggcgac gttccgcgtc gtcaacggca agcgttggtc
     2401 gatccccggc gacttcgccc gcgtcgaaga agacgggacg atagtgctgc tcggtcgcgg
     2461 ctcggtcgtg atcaactccg gcggcgagaa gatctacccc gaggaagtcg aggaggcggt
     2521 gaagctcaac cccgcggtcg ccgactgcct cgtcgtcggc gtgcccgacg accgcttcgg
     2581 cgaagcg
//
LOCUS       DASUGA010000175         4338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_673802, whole genome shotgun sequence.
ACCESSION   DASUGA010000175 DASUGA010000000
VERSION     DASUGA010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673802"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1746
                     /locus_tag="VFX21_03420"
     CDS             <1..1746
                     /locus_tag="VFX21_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5095030.1"
                     /translation="DGSLVALLLFARETFTAELTELVVYDSVDPGTAYVTTVGPGAQR
                     AAMIACDRADVDGRLARANVVLGSTMVAPFSGASGVSGAVLVADTLASGRTFKRDEER
                     LLRTFAGQLATTLEKAQINASLAKARVQKNEFAHQASHDALTGLANRSRFRQCLADGL
                     PAAAAAGTGLAALFIDLDDFKTVNDTMGHAAGDELLRVVAQRISASIGANDVAARLGG
                     DEFAVLLVDVDNDSDVQVVAERILVVLGDPIEIDGTRVIANASIGAAMHTAGEDADAL
                     MQNADIAMYTAKRNGKGRCDFFEPSLSFSGTRRRLVESGLQRALEAGELVLHYQPVVD
                     VAKNQIVAIEALVRWCDPSRGLVPPAEFIRVAEETSLIVPIGRFVLREACQQAKAWET
                     LAPEMRMFVNLSARELNEPDLADHVARVLAETGLEPSRLILEVTDAAVMQNMGAARRT
                     LNALKHLGVGLGLDDVGPGYAALQHLRHLPIDVLKIAKPWIDALGNTEADVTAVRELV
                     ELGHAVGLHVVAEGVERVDQAAELIDMGCEWAQGYYYARSLESEAIGTLLCRANARAG
                     ARRETKEQTELELAE"
     gene            1788..>4338
                     /locus_tag="VFX21_03425"
     CDS             1788..>4338
                     /locus_tag="VFX21_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5095031.1"
                     /translation="MHFRRRTPHLIGREQPLRALRGFFESADRVRPIALVTGEAGIGK
                     TRLLDEFASLADPAVVVRGGCVEDVAYAPWVDALWWVLEASSLSVVDGLSDRTRARLG
                     RLLPQLATDDSTGDEDNGQQLLFEAVLDLLGAVASTTELVVVIDDIHWIDPASADLLR
                     YLAENVGRVAVRLVVAYRPEESNGVSELLEDLERAEPLHIDLEPLAEDAVTQIASYLL
                     EEGATEAALRRIIETADGNPLFVEELVAAVQEEHVPHTLRDLMLVRFNALGDDACRLV
                     RIAAIIGARVSKEWLLAATEFPIEQAISAANEAVDAGILIDEHNGADVTFRHDLLRRS
                     VISDLVADELVVFHRAIARALTDHPERATEADYVAELARHWDAAEQAAPALKWMVAAG
                     RRADETYAFEAATSCYERALVWWDEADAPVTSAGIEHTELLLAAARANATAGHVDRGA
                     ELGRVALDETVALPPNEAIDAVARLYPIMWTADRAAELFEFASEHLEAHLDRAEPKVR
                     AHYLVKRVMYQLGRTDDAETRRIAARLLEDVEDVDDPGFESNVHEVMARCYEAWGEVG
                     KVDGEYELAASLARTAENFVMLTVIRYNHACYKLAVGDVDGCRRLLEDVDELVKQYGL
                     RRFLVPAACLRIVVLCLQGDLDGAREGYGRLVHEPLQGYDAWFRAYAGSLLTLFSGDF
                     GTAVAMLQPETVGVDAVTDADFGVEMAIVSADALTWMGDLGLARRAAESGLARLQDFY
                     EISSHGWITMVAMRVAADTADADRADALDEAWRNALANANAVSPLLRAYSAAAEYEHL
                     RARGHDSFTLARAAASEFASLGYGYYATYFRWREAHAVLGEGDRRIATELLA"
BASE COUNT          697 a         1437 c         1492 g          712 t
ORIGIN      
        1 gacggctcgc tcgtcgcgct gctcctgttc gcgcgcgaga cgttcacggc cgagctcacc
       61 gagctcgtcg tgtacgactc cgtcgacccc ggcaccgcgt acgtgacgac cgtcggcccg
      121 ggcgcgcaac gcgccgcgat gatcgcctgc gatcgcgcgg acgtcgacgg ccgcttggcg
      181 cgtgcgaacg tcgtgctcgg ctcgaccatg gttgcaccgt tctccggcgc gtccggcgtc
      241 tcgggtgcgg tgctcgttgc ggacaccctg gcgagtggtc gtacgttcaa acgggacgag
      301 gagcgactgc tccgcacctt cgccgggcag cttgccacca cgctcgagaa ggcgcagatc
      361 aacgcgtcgc tggcgaaggc ccgcgtacag aagaacgaat tcgcccacca ggcgtcgcac
      421 gacgcgctca cgggtttggc gaaccggagc cgcttccgcc agtgcctcgc cgacgggttg
      481 cctgccgcgg cggcggccgg cacgggcctg gcggcgttgt tcatcgacct cgacgacttc
      541 aagacggtga acgacaccat gggccacgcg gccggcgacg agttgctgcg agttgtggcg
      601 caacgaattt cagcgtcgat cggcgccaac gacgtcgccg cccgactcgg cggtgacgag
      661 ttcgcagtgc tgctcgtcga cgtggacaac gactccgatg tccaggtcgt cgccgagagg
      721 attctggtgg tgctcggtga cccgatcgag atcgatggca cgcgcgtgat cgcgaacgcc
      781 agcatcggcg cagccatgca tacagcgggc gaggacgccg acgccctgat gcagaacgcc
      841 gacattgcga tgtacacggc gaagcgcaac ggcaagggcc gctgcgactt cttcgagccg
      901 tcgttgagct tctcggggac gcgacgccgg ttggtcgagt cggggctcca gcgcgcgctc
      961 gaagccgggg agctcgtgct gcactaccag ccggtcgtcg acgtcgcgaa gaaccagatc
     1021 gttgcgatcg aagcactcgt gcgttggtgt gacccgtctc gagggctcgt accgcccgcc
     1081 gagttcatcc gcgtcgccga ggagacgagc ttgatcgtgc cgatcggccg gttcgtgctc
     1141 cgagaggcgt gtcagcaggc caaggcgtgg gagacgcttg cgccggagat gcgcatgttc
     1201 gtgaacctgt cggcgcgcga gctcaacgag cccgacctcg ccgaccatgt ggcccgtgtc
     1261 ctcgccgaga ccgggctcga accgagccgt ctcatcctgg aagtcaccga cgccgccgtg
     1321 atgcagaaca tgggcgcggc gcggcgcaca ttgaacgcgc tcaaacatct cggtgtcgga
     1381 ctcgggctcg acgacgtcgg tcctggctac gccgcgttgc agcacctccg gcatcttccg
     1441 atcgatgtcc tcaagatcgc caagccgtgg atcgacgcgc tcggcaatac ggaggcggac
     1501 gtgaccgccg ttcgggagct cgtcgagctc ggccatgccg tcggattgca cgtcgtcgcc
     1561 gagggtgtcg aacgagtcga ccaggccgcg gaactgatcg acatgggatg cgagtgggcg
     1621 cagggctact actacgcgcg ctcgctggag tccgaagcga tcggaaccct gctgtgccgg
     1681 gccaacgcgc gtgccggcgc acgccgggag accaaggagc aaaccgagct ggagctcgca
     1741 gagtaagagt tagcgggctc gggtacggat cgtccacaat tcgcaacgtg cactttcgcc
     1801 ggcgcacgcc gcacctgatc ggacgcgagc agccgctccg tgccctgcgc ggctttttcg
     1861 agagcgccga tcgtgtgcgc ccgatcgcct tggtgacggg cgaagcgggc atcggaaaga
     1921 cccgcctcct cgatgagttc gcgtcgctcg ccgatcccgc ggtcgtcgta cgcggcggtt
     1981 gcgtcgagga cgtcgcgtac gcgccgtggg ttgacgcgct ctggtgggtc ctcgaggcga
     2041 gcagcctgag cgtcgtcgat gggctctcgg accggacacg tgctcgactc ggtcggcttc
     2101 tgccacaact cgcgacggac gactcgaccg gcgacgaaga caacgggcag cagctgctct
     2161 tcgaggcggt cctcgacctc ttgggtgccg ttgcctcgac gacggaattg gtcgtcgtca
     2221 tcgatgacat ccactggatt gatcctgcga gcgcagatct gctccggtac ctcgccgaga
     2281 acgtcggtcg tgtcgccgtc cggctcgtgg tcgcgtaccg tccggaagag agcaacggtg
     2341 tcagcgagct cctcgaggat ctcgagcgcg ccgagcccct tcatatcgac ctcgagccgc
     2401 tcgccgagga cgccgtcacg cagatcgcct cgtacttgct cgaggagggc gcgacggaag
     2461 ccgctctccg ccgcatcatc gagaccgccg acgggaaccc gctgttcgtc gaggagctcg
     2521 tcgccgcagt gcaggaggag cacgttccgc acacgctccg cgatctcatg ctcgtacgct
     2581 tcaacgcgct aggtgacgac gcctgccggc tggtgcgcat tgcggcaatc atcggtgcgc
     2641 gagtctccaa ggagtggctg ctcgcggcga ccgagttccc gatcgagcag gcgatctcgg
     2701 ccgcaaatga ggccgtcgac gcaggcatcc tgatcgacga acacaacggc gccgacgtca
     2761 cgttccgaca cgacctgtta cgccggtcgg tcatctccga cctcgtcgcc gacgagctcg
     2821 tcgtgtttca tcgtgcgatc gcgcgtgcgc tcaccgatca tcccgagcgc gcgaccgagg
     2881 ccgactacgt ggccgagctt gcccgccact gggacgcggc ggagcaagcc gcgcccgcgt
     2941 tgaagtggat ggtggccgcg gggcgacgcg ccgacgagac gtacgcgttc gaggccgcga
     3001 cgtcatgtta cgagcgcgcc ttggtgtggt gggacgaagc cgacgccccc gtcacttcgg
     3061 ccggcatcga acacaccgaa ctactgcttg ccgcggcaag ggcaaacgcg acagcaggcc
     3121 atgtcgatcg cggcgccgag ctgggccggg tcgcattgga cgagaccgtc gcgctgccgc
     3181 ccaacgaggc gatcgatgcc gtggcgcggt tgtatccgat catgtggacg gccgaccggg
     3241 ccgcagagtt gttcgagttc gcgtcggagc acctcgaagc acatcttgac cgcgccgagc
     3301 cgaaggtgcg cgcgcactat ctcgtgaaac gcgtgatgta tcagttgggc cgcaccgacg
     3361 acgcggagac acgccgcatt gccgcgcggc tgctcgagga cgtcgaagac gtcgacgacc
     3421 cgggcttcga atcgaacgtg cacgaggtaa tggcccggtg ttacgaggcc tggggtgaag
     3481 tcggcaaagt tgacggcgaa tacgagttgg cggcttcgct cgctcgtacg gccgagaact
     3541 tcgtcatgtt gacagtcatc cgttacaacc acgcttgcta caagctcgcg gtcggcgatg
     3601 tcgacggttg ccgccggcta ctggaggacg tcgacgagct cgtcaagcag tacgggctgc
     3661 gacgattcct ggtccccgcg gcgtgcctcc gtatcgtcgt cctgtgcctg caaggtgatc
     3721 tcgacggggc gcgcgaagga tacgggcgcc tcgtgcacga gccgctgcag ggctacgacg
     3781 cgtggtttcg cgcgtacgcc ggctcgctgc tgacgctctt ctccggtgac ttcggcaccg
     3841 cggtcgcgat gctgcagccg gagaccgtgg gtgtcgacgc cgtcaccgac gccgacttcg
     3901 gtgtcgaaat ggcgatcgtc agcgccgacg cgctgacctg gatgggagac ctcggcctcg
     3961 cgcgccgggc cgccgagagc ggccttgcac ggttgcagga cttctacgag atctcgtccc
     4021 atgggtggat cacgatggtc gcgatgcgcg tcgcggccga cactgcggat gccgaccgcg
     4081 ccgacgcgct ggacgaggcg tggcgcaatg ccctcgccaa cgccaacgcg gtgtcacctc
     4141 tcttgcgcgc gtacagcgcc gcggccgagt acgagcacct gcgtgcgcgc gggcatgact
     4201 cgttcacgct cgcgcgcgcg gcggcgagcg aattcgcgag cctcggctac ggctactacg
     4261 cgacgtactt ccgttggcgg gaagcacacg cggtgctcgg cgagggcgat cgccggatcg
     4321 cgaccgagtt gctcgccg
//
LOCUS       DASUGA010000176         2555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_592411, whole genome shotgun sequence.
ACCESSION   DASUGA010000176 DASUGA010000000
VERSION     DASUGA010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592411"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..1020)
                     /locus_tag="VFX21_03430"
     CDS             complement(241..1020)
                     /locus_tag="VFX21_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX5095032.1"
                     /translation="MPEILEVEAARAVLEAHALDRKIESVHAPDAWFLKRGATSEDLT
                     DVLVGEAFTTARRRGKSMVLDTSGGARVGLHLGMSGRVIVDDEEAGDPLVYASNRRVE
                     AWHRFGVNFADGGSFFLRDPRRLGGVELDPDESRLGPDAFTLTVKELDHALGTRTAPV
                     KAVLMDQARIAGLGNLLTDEALWRAGVDPERPAYELDLDTRRQLQRAIRTALRTLGRR
                     GGSHTGDMPRGRDAVCPKDGAELVRRTVGGRTTYSCPVHQR"
     gene            complement(1025..>2555)
                     /gene="ilvD"
                     /locus_tag="VFX21_03435"
     CDS             complement(1025..>2555)
                     /gene="ilvD"
                     /locus_tag="VFX21_03435"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX5095033.1"
                     /translation="PCNLPLARLAQRAKAGVRAFGGFPLEFVTIAVSDGISMGHEGMR
                     GSLVSREIIADSVECVMHAERLDALVTFAGCDKSLPGMLMAAARLNLPSVFLYGGSIL
                     PGHYKDQALDIVSVFEAVGACAAGTLTENELGEIEKRACPTEGSCAGMFTANTMSSIG
                     EAIGLALPGSASPPAVDRRRDDFAYESGRAVMRLLELDLRPRQILTREAFENAIAVTM
                     ALGGSTNAVLHLLAIANEARVELELEDFNRIGARVPHIADMKPHGNFHMVDLDAIGGV
                     PVVMKALLDAGLLHGDCLTVTGKTVAENLADLDPPAIDGRVVMPLSAPIHAQGGIAVL
                     RGSLAPNGAVVKIAGIDELRFDGVARVFDGESAAMEAILAGKINAGDIVVIRYEGPKG
                     GPGMREMLAITGAMKGAGRGADAALITDGRFSGGTHGFCVGHVAPEAVDGGPIAFVQE
                     GDRIVIDAGEYTVDLLVDDKELAERKKNWKPRDPLYTSGFLAKYARLAQGAEKGAITN
                     I"
BASE COUNT          381 a          890 c          885 g          399 t
ORIGIN      
        1 gacctgctcc atctccaggc tcttgatgag ggcttgggct ccggtcagct tcatggtttc
       61 tctcttcggc ggattgcgga cgtgaaaaac ccctcgcacg ggcgaggggc ggaagagcgc
      121 acgggcgcgt gaagggacga ccgtgcgcta cgtaattact acgaggaggt cgcggccggt
      181 gggcatcaag gagagagtat cacccgaatg gggcggccct ggcaagcccg ccacggccgg
      241 tcagcgctgg tgcaccgggc acgagtacgt cgtacggccg ccgacggtac ggcgcaccag
      301 ctcggcaccg tccttggggc acaccgcgtc gcgcccgcgc ggcatgtcgc cggtgtggct
      361 gccgccccgc cgcccgagcg tgcgcaacgc cgtgcggatc gcgcgctgga gctgtctgcg
      421 ggtgtcgagg tcgagctcgt acgcgggacg ctcgggatcg acgcccgcgc gccacagggc
      481 ttcgtctgtg agcaggttgc cgaggccggc gatgcgggct tggtccatca gcactgcttt
      541 caccggcgcg gtgcgcgtac cgagcgcgtg gtcgagctcc ttcacggtga gcgtgaacgc
      601 gtcgggcccc aggcgcgact cgtccgggtc gagctcgacg ccgccgagcc ggcgcggatc
      661 gcgcaggaag aacgagccgc cgtcggcgaa attcacaccg aagcgatgcc acgcctcgac
      721 ccgccggttg ctcgcgtaga cgagcgggtc accggcctcc tcgtcgtcga cgatgacgcg
      781 gccgctcatc ccgagatgca gcccgacgcg tgcgccaccc gaagtgtcga gcaccatcga
      841 cttgccccgc cggcgtgccg tcgtgaacgc ctcccccacg agcacatcgg tcaagtcctc
      901 cgatgtggct cctcgcttga gaaaccacgc gtcgggcgcg tgcaccgact cgatcttccg
      961 gtcgagtgca tgcgcttcga ggaccgcgcg cgcggcttcg acctcgagaa tctccggcac
     1021 cgggctagat gttcgtgatc gcgccctttt cggcgccttg cgcgaggcgc gcgtacttgg
     1081 cgaggaagcc gctcgtgtac agcgggtcgc gcggcttcca gttcttcttg cgctccgcga
     1141 gctccttgtc gtcgacgagg aggtcgaccg tgtattcgcc cgcgtcgatg acgatgcggt
     1201 caccctcttg cacgaacgcg atcggtccgc cgtcgacggc ttcgggggcg acgtgcccga
     1261 cgcagaaccc gtgcgtgccg cccgagaagc ggccgtcggt gatgagcgcg gcgtcggcgc
     1321 cgcggcccgc gcccttcatc gcaccggtga tcgccagcat ctcgcgcatc ccgggcccgc
     1381 ccttgggacc ctcgtagcgg atgacgacga tgtcgcccgc gttgatcttg cccgccagga
     1441 tcgcctccat cgcggccgac tcaccgtcga agacgcgcgc aacaccgtcg aagcgcagtt
     1501 cgtcgatgcc ggcaatcttc acgaccgcgc cgttgggcgc gagcgacccg cgcaacaccg
     1561 cgatcccgcc ctgggcgtga atcggcgccg acagcggcat cacgacccga ccgtcgatgg
     1621 ccggcgggtc gaggtcggcg aggttctcgg cgacggtctt ccccgtcacg gtgaggcagt
     1681 cgccatgcag caggcccgcg tcgaggagcg ccttcatcac gaccggcacg ccaccgatcg
     1741 cgtcgaggtc gaccatgtgg aagttgccgt ggggcttcat gtcggcgatg tgcggcacac
     1801 gcgcgccaat gcggttgaag tcctcgagct ccagctcgac gcgcgcttcg ttcgcgatcg
     1861 cgaggaggtg cagcaccgcg ttggtcgagc cgccgagcgc catcgtgacc gcgatcgcgt
     1921 tctcgaatgc ttcgcgcgtg aggatctgcc gcggccggag gtcgagctcg agcagccgca
     1981 tgaccgcgcg gcccgactcg taggcgaagt cgtcgcgccg gcggtcgact gcgggcggcg
     2041 acgcggatcc cggcaaggcg agcccgatcg cctcaccgat cgacgacatc gtgttggcgg
     2101 tgaacatgcc cgcgcacgat ccctcggtcg ggcacgcgcg cttctcgatc tcgcccagct
     2161 cgttctcggt gagcgtgccc gccgcgcacg caccgacggc ctcgaacaca ctgacgatgt
     2221 cgagggcttg gtccttgtag tgaccgggaa ggatcgagcc gccgtacagg aagaccgatg
     2281 gcaggttgag tcgggccgcg gccatgagca tcccgggaag gctcttgtcg catcccgcga
     2341 aggtgacgag cgcgtcgagg cgctccgcgt gcatgacgca ctcgaccgag tcggcgatga
     2401 tctcacggga cacgagcgat ccccgcatgc cctcgtggcc catcgagatg ccgtcgctca
     2461 ccgcgatcgt gacgaattcg agcgggaagc ctccgaaggc gcgcacgccg gccttcgccc
     2521 gttgggcaag gcgcgccagc ggcaggttgc acggc
//
LOCUS       DASUGA010000177         5608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1201655, whole genome shotgun sequence.
ACCESSION   DASUGA010000177 DASUGA010000000
VERSION     DASUGA010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201655"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VFX21_03440"
     CDS             <1..790
                     /locus_tag="VFX21_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5095034.1"
                     /translation="LVITAIAMTEPGIGSDLASMATSAILDGDHYVVNGSKTFITNGI
                     NADLVITAVKTDPTQKHKGISLLILERGMEGFERGRNLEKLGLHSQDTAELFFNDVPV
                     PVNNLLGEEGQGFLHLVHNLPQERLSIAVAGVAAARAALDWTLEYVKQRTAFGQPVGT
                     FQNSRFVLAEVATEVEVGQAFVDRCVLALNDDTLTAEEAAMAKWWCTELQKRVVDKCL
                     QLHGGYGYMLEYPIARAYADARVTTIYGGTTEIMKEIVGKSLGL"
     gene            829..1683
                     /locus_tag="VFX21_03445"
     CDS             829..1683
                     /locus_tag="VFX21_03445"
                     /inference="COORDINATES: protein motif:HMM:NF022655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LmeA family phospholipid-binding protein"
                     /protein_id="HEX5095035.1"
                     /translation="MPLDPWASAASVAGWMERTASWVSPLVPTPSRMLRDFLEAIAGR
                     YTGQTVTLEVAAGTTLTGTLDAIRLRANEEMGGLAPAREMVRWTKRVAGLPSDDPPMV
                     EAVEIESTDVEVNGRSIGRVVADADGIRLDPGITPVLITGPISLRIIASYIDAVAWVA
                     HELPEWQITRYRDDLLAVRGPGWKVTAVVRATFTPDLKLKTEPVGVLVFGKMRGVPRR
                     LVDKLTQVLDIPPLGARLTLKELRVEGERVHMKLHHPGIRQPVHLDMIRAAVRDGVAT
                     LANQAFAP"
     gene            complement(1687..2541)
                     /locus_tag="VFX21_03450"
     CDS             complement(1687..2541)
                     /locus_tag="VFX21_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5095036.1"
                     /translation="MIDLVGRDGNRIAADVAGDPADPPVVLLHGGGQTRHSWGTTLTA
                     LADKGWHAFSMDLRGHGESEWAADGDYTLDAFAGDILAIARTFDRPPALVGASLGGLS
                     CLAAIGEHPDEAVARALVLVDVAPKIEDEGRERIGMFMIEHMNDGFGSLEEVADAIAA
                     YNPHRPRPSNLEGLKKNLRERAGRWYWHWDPTFIVGRMGGADETRSSLIDPHRLEHAA
                     RALTVPTLLVRGRVSDLLSEQGAQEFLQLAPHAQMVDVSGAGHMVAGDRNDLFNDAVV
                     AFLDGVRD"
     gene            complement(2538..3740)
                     /locus_tag="VFX21_03455"
     CDS             complement(2538..3740)
                     /locus_tag="VFX21_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX5095037.1"
                     /translation="MSEDIWILGIHMTKFGKHPDLDTVDLASEAARGALADGGVQMRQ
                     MGIIAAGNLMGGGLGIAQAIQKQIGQTGIPAYNVSNACATGATALRTAIMAVKAGECD
                     YGLAVGVEKLSGAGLLSGGMRAGDADTWSPKGRYGAVAAVDGRVGTETMPGVFAQIGM
                     EYGHRYGGVDFDLFAGISEKNHAHSTLNPLASYQKRFTLDEIKNDVMIAYPNTRPMCS
                     ANCDGAAAAVVVNDSTLKKLSKEQQRRAVKVSASILTTDPWEEGCQVLPNVNTLTRNA
                     AKQAYELAGVGPSDLDLVELHDCFATAELVHYDNLMLCEEGGAGDFFRSGATWRDGTS
                     PVNVSGGLESKGHPIAATGIANIWEVCHHLRGEAGDRQIENAKVGLAHVIGLGSACGV
                     HILERSAA"
     gene            complement(3737..4144)
                     /locus_tag="VFX21_03460"
     CDS             complement(3737..4144)
                     /locus_tag="VFX21_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX5095038.1"
                     /translation="MPDQIPLVDYLALTPEPHLVAHECVACGARYFDRRNACAACEAD
                     EFKDVALATTGEVRAYTIVSLAAPGIPVPFVAALVDCDGTSVRANVINTEPDPDHVKV
                     GMKVRLTTQLVGVDAAGTEAIGFGFEPLEGASK"
     gene            4219..4743
                     /locus_tag="VFX21_03465"
     CDS             4219..4743
                     /locus_tag="VFX21_03465"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEX5095039.1"
                     /translation="MTFAEPRVPGTDGDEREVLLGFLHWLRELVVATADGLTEDELRW
                     TPDDRLLPILGIVNHLTHVEWRWIEGRYLQAPFPPRGEEFVVGREVTGSDVIAAYWTR
                     AQRTAEVVRDAPSLTVECVGQEGAMPPAHALLGLERPIDLRWVMLHLIEETGRHAGHA
                     DATREMLDGKRTSG"
     gene            complement(4874..>5608)
                     /locus_tag="VFX21_03470"
     CDS             complement(4874..>5608)
                     /locus_tag="VFX21_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018771361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX5095040.1"
                     /translation="ALADPTRRQLIDELFREDGQTLSALEERFPMTRFGVMKHLRQLE
                     DAGLVVTKKRGREKLHFLNPVPIRLVHDRWVSKYAEPWVAGLSDLKQQLEQTMEKVFE
                     IYIRTTPERLWEAITNPETRRKYNFGVGAESDWKQGSTIKMVHPQGGLELGDGKVLEV
                     DPPRRLVHTMTALWSDEVKAEGESRVTWEIEPVGDSCRLLLTHDELREGANDELYGGW
                     PMILSGLKTWLETGELLTTPGSLMYT"
BASE COUNT          863 a         1907 c         1907 g          931 t
ORIGIN      
        1 cctcgtcatc accgcgatcg cgatgaccga gcccgggatc gggtcggacc tcgcgtcgat
       61 ggcgaccagc gcgatcctcg acggcgacca ctacgtggtg aacgggtcca agacgttcat
      121 cacgaacggc atcaacgccg acctcgtcat caccgcggtg aagaccgacc cgacgcagaa
      181 gcacaagggc atctcgctgc tcatcctcga acgcgggatg gagggcttcg agcgcggccg
      241 caatctggag aagctcggcc tgcactccca ggacactgcg gagttgttct tcaacgacgt
      301 gccggtgccc gtgaacaacc tgctcggtga agaggggcag gggttcttgc acctcgtgca
      361 caacttgccg caggagcggt tgtcgatcgc agttgccggt gtcgcggccg cccgcgccgc
      421 gctcgactgg acgctcgagt acgtgaagca gcgcaccgca ttcggccagc cggtcggtac
      481 gttccagaac agccggttcg tgctcgccga agtcgcgacg gaggtcgaag tcgggcaggc
      541 gttcgtcgac cgctgcgtgc tcgcactgaa cgacgacacg ctcaccgcgg aggaagccgc
      601 gatggcgaag tggtggtgca ccgagctgca aaaacgcgtc gtcgacaagt gcctgcagct
      661 gcacggcggc tacggctaca tgctcgagta cccgatcgcg cgtgcgtacg ccgacgcgcg
      721 cgtcacgacg atctacggcg ggacgacgga gatcatgaaa gagatcgtgg gcaagtcgct
      781 cggtctctga cggcgacgat gaccgactcc tcgtcatccg gacgctggtt gccgctggat
      841 ccctgggcgt cggcggcgag tgtcgcgggc tggatggaac gcaccgcgtc gtgggtgtcg
      901 ccgttggtac cgacgccgtc gcggatgttg cgcgacttcc tcgaagcgat cgccggtcgt
      961 tacacgggcc agacggtcac gctcgaagtc gcggccggga caacgctgac cggcacactt
     1021 gatgccatcc ggctgcgcgc caacgaggag atgggcggcc tcgcacctgc acgcgagatg
     1081 gtgcgctgga ccaagcgcgt cgcgggcctc ccgagcgacg atccgccgat ggtcgaagca
     1141 gtcgagatcg agagcaccga cgtggaggtc aacgggcgct cgatcggtcg tgtcgtcgcg
     1201 gacgccgacg gcatccggct cgaccccggc atcacgccgg tattgatcac cggcccgatc
     1261 tcgttgcgca tcatcgcgag ctacatcgat gccgtcgcat gggtcgcgca cgagctcccg
     1321 gagtggcaga tcacccgcta ccgcgacgat ctccttgcgg tacgcggtcc gggttggaag
     1381 gtcacggccg tcgtgcgcgc gacgttcact cccgatctga agttgaagac ggaaccggtc
     1441 ggggtgctcg tgttcggcaa gatgcgcggg gtaccgcgtc ggctcgtcga caagctgacg
     1501 caggtgctcg acatcccgcc gctcggtgcg cgactgacat tgaaggaact gcgggtcgaa
     1561 ggggaacggg tgcacatgaa gctgcaccat cccggtattc gtcagccggt gcacctcgac
     1621 atgatccggg ccgcggtccg cgacggtgtc gcgacgctcg cgaaccaggc gttcgcgccc
     1681 tgacggtcag tcgcgcacgc cgtcgaggaa ggcgacgacc gcgtcgttga acaagtcgtt
     1741 gcggtcgccg gcgaccatgt gtcccgcgcc cgatacgtcg accatctgcg cgtgcggtgc
     1801 gagctggagg aactcctgcg cgccctgctc gctcaacagg tcgctgacgc ggccgcgcac
     1861 gagcagcgtc ggcaccgtca gcgcccgggc cgcgtgctcg agccggtgcg ggtcgatgag
     1921 cgacgaacgc gtctcgtccg cgccgcccat gcggccgacg atgaacgtcg gatcccagtg
     1981 ccagtaccac cggccggcgc gctcgcgcag gttcttcttc aaaccctcga ggttcgacgg
     2041 ccgcggccgg tgcgggttgt acgccgcaat cgcgtccgcg acctcttcga gcgacccgaa
     2101 cccgtcgttc atgtgctcga tcatgaacat gccgatgcgc tcacggcctt cgtcttcgat
     2161 cttgggcgcg acgtcgacga gcaccaacgc gcgggcgacc gcttcgtcgg gatgctcgcc
     2221 gatcgccgca agacaggaga ggccgccgag cgacgcgccc accaatgcgg gtgggcggtc
     2281 gaacgtccgc gcgatcgcga ggatgtcgcc cgcgaacgcg tcgagcgtgt agtcgccgtc
     2341 ggccgcccac tcgctctcgc cgtgaccgcg caggtccatc gagaacgcgt gccagccctt
     2401 gtcggccagc gcggtcagtg tcgtgcccca cgagtggcgc gtctgcccgc cgccgtgcag
     2461 gagcacgacg ggcgggtcgg ccggatcgcc ggcgacgtcc gccgcgatcc ggttgccgtc
     2521 ccgcccgacg aggtcgatca cgcggccgat cgttcgagga tgtgcacccc gcacgcagag
     2581 ccgaggccga tcacgtgcgc gaggccgacc ttcgcgttct cgatctggcg gtcgcccgcc
     2641 tcgccgcgca ggtggtggca gacctcccag atgttcgcga tgccggtggc tgcgatcggg
     2701 tggcccttgg actcgaggcc gccggacacg ttgaccggcg acgtgccgtc gcgccacgtc
     2761 gcacccgaac ggaagaagtc gcccgcgccg ccctcttcgc acagcatcaa gttgtcgtag
     2821 tgcaccagct ccgcggtcgc gaagcagtcg tgcaactcga cgaggtcgag atcgctcggg
     2881 ccgacacccg cgagctcgta cgcctgcttg gccgcgttgc gcgtcagcgt gttgacgttg
     2941 gggaggacct ggcagccctc ttcccagggg tcggtcgtca ggatcgacgc gctcaccttg
     3001 accgcgcgcc gctgctgttc cttcgacagc ttcttcagcg tgctgtcgtt gacgacgacc
     3061 gcggccgccg cgccgtcgca gttggccgag cacatgggcc gcgtgttcgg gtacgcgatc
     3121 atcacgtcgt tcttgatctc gtccagcgtg aagcgcttct ggtagctcgc gagcgggttg
     3181 agcgtcgagt gcgcatggtt cttctcggag atgcccgcga acaggtcgaa gtcgacgccg
     3241 ccgtagcggt ggccgtactc catgccgatc tgcgcgaaca cgcccggcat cgtctcggtg
     3301 ccgacgcggc cgtcgaccgc ggcgaccgcg ccgtagcggc ccttcggcga ccacgtgtcg
     3361 gcgtcgccgg cccgcatgcc gcccgagagc aggcccgcgc ccgagagctt ctcgacaccg
     3421 accgcgagcc cgtagtcgca ctcgccggcc ttcaccgcca tgatcgcggt gcgcaacgcg
     3481 gtggcgcccg tcgcgcacgc gttggacacg ttgtacgcgg ggataccggt ctggccgatc
     3541 tgcttctgga tcgcctgcgc gatgccgagc ccgccgccca tcaggttgcc ggccgcgatg
     3601 atgcccatct ggcgcatctg cacaccgccg tcggcgagcg cgccgcgcgc ggcctcgctg
     3661 gcgaggtcga cggtgtcgag gtcggggtgc ttcccgaact tcgtcatgtg gatgccgagg
     3721 atccagatgt cttcgctcac ttcgacgctc cttccagagg ctcgaatccg aacccgatcg
     3781 cttcggtgcc cgcggcatcg acgccgacga gctgcgtcgt gagccgcacc ttcataccga
     3841 ccttcacgtg gtcgggatcg ggctcggtgt tgatcacgtt ggcgcgcacg ctcgtgccgt
     3901 cgcagtcgac gagcgcggcg acgaacggca ccgggatgcc gggtgcggcg agcgacacga
     3961 tcgtgtacgc gcgcacctcg ccggtcgtcg cgagcgcgac gtccttgaac tcgtccgcct
     4021 cacacgcggc acatgcgttg cgccggtcga agtagcgcgc tccacacgca acgcactcgt
     4081 gcgcgacgag gtgcggttcg ggcgtgagcg cgagatagtc gaccagcggg atctggtccg
     4141 gcatacggcc tccaggtgtc gagtcagggc tctctctacc aggacaaggg ggtcgcgcgg
     4201 gaatatacgc tcggccctat gaccttcgcc gagccgcgtg ttcccggtac cgacggcgac
     4261 gagcgggagg tgctgctcgg gttcctccac tggctgcgcg agctcgtcgt cgcgaccgcc
     4321 gacggcttga ccgaggacga actgcgctgg acgccggacg accgtctcct gccgatcctc
     4381 gggatcgtca accacctgac ccatgtcgaa tggcgatgga tcgaaggccg ctacctgcag
     4441 gcgccgttcc cgccccgggg cgaggagttc gtggtcggac gggaagtgac cggttccgat
     4501 gtgatcgccg cgtattggac gcgtgcccag cgcaccgccg aagtcgtgcg cgatgcgccg
     4561 agcctgaccg tggagtgcgt cgggcaggaa ggtgcgatgc cgcccgcgca cgcgctgctc
     4621 ggactcgaac gtcccatcga cctgcgctgg gtgatgctcc acctgatcga ggaaaccggg
     4681 cgtcacgccg gccacgccga cgcgacccgc gagatgctcg acgggaagcg cacatccggc
     4741 tgaagggtgg gcacgtgctg agcgttctga cgcgccgcgt gccgtgccgg agcttgcgga
     4801 ggcactggcg gagtcctgag gcgggccaac aatccgggct gcctcacgcg agcacgtcga
     4861 aggcgacaaa gagttacgtg tacatcagtg aacccggggt ggtgagcagt tcgccggtct
     4921 cgagccaggt cttcaagccg gagaggatca taggccagcc gccgtagagc tcgtcgttcg
     4981 cgccttcgcg caactcgtcg tgggtgagga gcaaccggca cgagtcgccg accggttcga
     5041 tctcccacgt cacgcgcgac tcgccttcgg ccttcacctc gtcgctccac agcgcggtca
     5101 tcgtatgcac gagccggcgc ggcggatcga cctcgagcac tttgccgtcg ccgagctcca
     5161 ggccgccctg cgggtgcacc atcttgatcg tggagccctg cttccaatcc gactcggcgc
     5221 cgacaccgaa gttgtacttg cggcgcgtct cgggattggt gatcgcttcc cacaagcgtt
     5281 cgggtgtcgt gcggatgtag atctcgaaca ctttttccat tgtctgctcc agttgttgct
     5341 tgaggtcgct gaggcctgcg acccacggtt ccgcgtactt gctcacccac cggtcgtgca
     5401 ccaggcggat ggggacggga ttcaggaagt gcaacttctc gcggccgcgc ttcttggtga
     5461 ccacgagccc cgcgtcttcc agctgcctca ggtgcttcat cacgccgaag cgcgtcatcg
     5521 ggaagcgttc ttcgagcgcg ctgagcgtct gcccgtcctc gcggaacagc tcgtcgatca
     5581 gctgccgccg ggtcgggtcg gccaaggc
//
LOCUS       DASUGA010000178         2535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1426058, whole genome shotgun sequence.
ACCESSION   DASUGA010000178 DASUGA010000000
VERSION     DASUGA010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426058"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFX21_03475"
     CDS             <1..258
                     /locus_tag="VFX21_03475"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX5095041.1"
                     /translation="PYASAVLAQAARSKARFVGMMSSRRHVAHHLDAVRALGLPEEAI
                     ARIHAPVGLDLGRKDADGIALSIAAGIVADANETDGGWLDR"
     gene            341..1723
                     /locus_tag="VFX21_03480"
     CDS             341..1723
                     /locus_tag="VFX21_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX5095042.1"
                     /translation="MRKAVAALVAICSVVAAAVFRGSLPVVARDPRPNVLLVLTDDQT
                     LDTMTSDPPAMPWLRSQLTDPAGHWLWFPRAVASTPLCCPSRATILTGRYDTQTGVRN
                     NGQGPNLDDTNTLPVWLDDAGYTTGLVGKYLNFYPWDRAPFVPPGWDRWFAKENADES
                     TAYYDYDVVDQGIVRHYGDAPTDYATDVLGAQALRFVQDAPSDAPWFLYFSPNAPHLP
                     WIPAPAHTGAFDDADPPFPDLGVMNDVRGKPAYVRDLPPKTEADRAASIQDDRNERAM
                     LLSVDAWFHEIVDAIASRGELDTTVIVFMGDNGYTLGLHRLDGKRYPYTPSVRVPFAI
                     RTPWAPAGTVGDPVSNVDVAGTIAALAGATPGVEQMGIDLGPAIRGDPLPPRDGVFLD
                     WGGDVNGPPWQGVLTRDGTYVRNADGFRELYRARDRWQLHNVAGAPGAQALARRDRSL
                     LSLLGAEAQG"
     gene            1733..>2535
                     /locus_tag="VFX21_03485"
     CDS             1733..>2535
                     /locus_tag="VFX21_03485"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5095043.1"
                     /translation="MQNGPPIIRRSNLRRPTRSQIARRRAVVVVAVVVGLIALWQFWP
                     SGGTEGAAGPGPSETGSETVSPTASPSSSIKPNANPIQHVIFLVKENHTFDNYFGKYP
                     GADGATRGKTLQCDQGNTPGPTVKLTQAPYSYPHDLGHAFVPGLTAIDGGKMDGYNCV
                     PLGDDLTGYTQYDRKSLPAYWALADRFVLADHFFTSMYGPTFPEHLYTVAAQSYGIVD
                     NKSSTDHPGSYCDDPTEYTSRFPIEDLSNTDVRTIMNLEDHVLDNPANL"
BASE COUNT          402 a          937 c          857 g          339 t
ORIGIN      
        1 ccgtacgcca gcgcggtgct cgcacaggcc gcgcgctcga aggctcggtt cgtcgggatg
       61 atgagctcac gccgccacgt cgcccaccat ctcgacgcgg tgcgcgcgct cggtctcccg
      121 gaggaggcga tcgcccgcat ccacgcgccc gtcgggctcg acctcggccg caaggacgcc
      181 gacgggatcg cgctgtcgat cgccgccggc atcgtcgccg acgcgaacga gaccgacggc
      241 ggctggctcg accgatgaac cgagccggcg ctcgcgcgag ccgccgcacc ggcagtgaca
      301 gtcccgcgga gcggatccgg aaggtgggga aggaggcggc gtgaggaagg cggtcgccgc
      361 gctggtggcg atctgcagcg tcgtcgcggc ggccgtgttc cgcgggagcc tgccggtggt
      421 cgcccgtgac ccgcgtccga acgtcctgct cgtcctgacc gacgaccaga ccctcgacac
      481 catgacgtcg gacccgccgg cgatgccgtg gctcaggtcg cagctgaccg acccggccgg
      541 ccactggctc tggttcccgc gcgcggtcgc ctcgacgccg ctgtgctgtc cctcccgcgc
      601 gacgatcctc accggccgct acgacacgca gaccggcgtg cgcaacaacg ggcaaggtcc
      661 caacctcgac gacacgaaca cgctgccggt gtggctcgac gacgccggct acaccaccgg
      721 cctggtcggc aagtacctca acttctaccc gtgggaccgc gcgccgttcg tgccgccggg
      781 atgggaccgc tggttcgcca aggagaacgc cgacgagtcg accgcctact acgactacga
      841 cgtcgtcgac cagggcatcg tgcggcacta cggcgacgcg ccgacggact acgcgacgga
      901 cgtgctcggc gcgcaggcgc tccggttcgt gcaggacgcg ccgagcgacg ccccgtggtt
      961 cctgtacttc agcccgaatg cgccgcatct gccgtggata ccggcgcccg cgcatacggg
     1021 cgcgttcgac gacgcggacc ccccgttccc ggatctcggg gtgatgaacg acgttcgcgg
     1081 caagcccgcg tacgtccggg atctgccgcc gaagacggaa gcggaccgcg ccgcctccat
     1141 ccaggacgat cgcaacgagc gcgcgatgct gctgtcggtc gacgcgtggt tccacgagat
     1201 cgtcgacgcg atcgcgtccc gaggcgagct cgacaccacc gtgatcgtct tcatgggcga
     1261 caacggctac acgctcgggc tgcaccggct cgacgggaag cggtacccgt acacaccgtc
     1321 ggtgcgcgtg ccgttcgcga tccgcacgcc gtgggcgccg gcggggacgg tcggcgaccc
     1381 ggtctccaac gtcgacgtcg cgggcacgat cgccgcgctc gccggcgcga cgcccggcgt
     1441 cgagcagatg ggcatcgatc tcggcccggc catccgcggc gatccgctgc ccccgcgcga
     1501 cggggtcttc ctggactggg gcggggacgt gaacggcccg ccgtggcaag gcgtgctgac
     1561 acgcgacggc acctacgttc gcaacgccga cgggttccgg gagctgtacc gggcgcgcga
     1621 ccggtggcag ctgcacaacg tcgccggcgc gccgggcgcg caggcgctcg cacgccgcga
     1681 ccggtcgttg ctgtcgctcc ttggcgcgga agcgcagggg tagactcgcg cggtgcagaa
     1741 cgggccgccg atcatcagac gatcgaacct gcggcggccg acccgcagcc agatcgcgcg
     1801 acgacgcgcc gtggtcgtcg tcgcggtcgt cgtcgggctg atcgcgctgt ggcagttctg
     1861 gccgagcggc ggcaccgagg gcgccgccgg tccgggaccg agcgagacgg gctccgagac
     1921 ggtgagcccc acggcctcac ccagcagctc gatcaagccg aacgccaacc cgatccagca
     1981 cgtcatcttc ctggtgaagg agaaccacac cttcgacaac tacttcggca agtatcccgg
     2041 cgccgacggc gccacgcgcg gcaagaccct gcagtgcgac caggggaaca cgcccggccc
     2101 gaccgtcaag ctcacacagg cgccgtactc gtacccgcac gatctcgggc acgcgttcgt
     2161 ccccgggttg accgccatcg acggcggcaa gatggacggc tacaactgcg tcccgctcgg
     2221 cgacgacctg accggctaca cgcagtacga ccggaagagt cttccggcgt actgggcgct
     2281 cgccgaccgg ttcgtgctcg ccgatcactt cttcacgtcg atgtacggcc cgacgttccc
     2341 cgagcacctc tatacggtgg cggcgcagtc ctacgggatc gtcgacaaca agagctcgac
     2401 cgaccacccg gggagctatt gcgacgatcc caccgagtac acgagccggt tccccatcga
     2461 ggacctctcg aacaccgacg tgcgcacgat catgaacctc gaggatcacg tcctcgacaa
     2521 ccccgcgaac ctgtt
//
LOCUS       DASUGA010000179         2747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1704378, whole genome shotgun sequence.
ACCESSION   DASUGA010000179 DASUGA010000000
VERSION     DASUGA010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704378"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1266
                     /locus_tag="VFX21_03490"
     CDS             94..1266
                     /locus_tag="VFX21_03490"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5095044.1"
                     /translation="MASPKRPYPGVPVVDGDGHILEPRDLWERELPAKFQDLAIKIVW
                     NEDTQKEDEFVQNVNVAPGVGTCNGWARLPRSVRDDPVGLRWEDLTPAGLYPKDRLIE
                     LDREGIDAAVLYPSLGLGIGGLVDPEHAVLACRIYNDWLAEYCATDPARLIGIGAVPM
                     QDPKEAPREAERCVRELGFRGVFIRPNPILDMWPHHRVYDELWSTVEGLGVPVGFHPA
                     GFPDTFGAARTYGPEWQGITPLGKVINFMIDVVNTFTMMIAAGTLDRCPELKLIVLES
                     GCGWLHWWLDKMDHWNEARLAGPGMELKPSEYVERQIWVSGDPDESSFGSVSEVAPPG
                     RLMWASDFPHLDILESEPSCTEELYEQLESLPRDVAEGILGYNACDVYGLDVRAGG"
     gene            1266..1550
                     /locus_tag="VFX21_03495"
     CDS             1266..1550
                     /locus_tag="VFX21_03495"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX5095045.1"
                     /translation="MHAIIITRMVVHADQVDRASEVFRAMQADVRAQEPGTVFYAYYQ
                     ADDDPTVFWVHEVFAHDAAKQFHLGNHAHRRADFDAILAQPPEFHMVHEL"
     gene            1562..2440
                     /locus_tag="VFX21_03500"
     CDS             1562..2440
                     /locus_tag="VFX21_03500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX5095046.1"
                     /translation="MRLGVALGNLGLAIGRPLEGAEHGALVYRIAEAAEELGFHSVWA
                     GDHLALPRTPTTPYPYGDDAALLSADVSLLDPFAVLAAVAGRTERVRLGFGVAVLPYR
                     HPLVVAKLVASIDALSNGRVILGVGTGWMPEEFDAVGADFGARGRDTDAALAYLRRVF
                     ADGEADDLTVLPVPVQRPGPPIWVGGVAPAALRRAVEFADGWNAPYADPERLANGIER
                     LHAECRIAGRDAESIDISVHGIAADDVDDALLDAYAALGVTDLGVRLPLADAGSPVHA
                     LRDLATRCPRVLDHIG"
     gene            2489..>2747
                     /locus_tag="VFX21_03505"
     CDS             2489..>2747
                     /locus_tag="VFX21_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095047.1"
                     /translation="MKRVLASVAVVALVATSLLATAGAAGAVVRSAADLAADCNDDGI
                     VSITGDTTYVGGTGEITGTQLFPGSAPICMIDVEAATVTLKI"
BASE COUNT          414 a          922 c          965 g          446 t
ORIGIN      
        1 ccggctcgac gacgtgatcg tcgacgctga tgatcttcgg cagctccatt cgaagcagcg
       61 tatccgggag ttcatctgga atacggtggc gggatggcgt caccgaagcg tccgtatccc
      121 ggagtgccgg tcgtcgacgg cgacgggcac atcctcgagc cgcgtgacct ctgggagcgc
      181 gagctccccg cgaagttcca ggacctcgcg atcaagatcg tgtggaacga ggacacgcag
      241 aaagaggacg agttcgtcca gaacgtgaac gtcgccccgg gtgtcggcac gtgcaacggc
      301 tgggcgcgcc tcccgcgctc cgtgcgcgac gatccggtcg ggttgcggtg ggaggatctc
      361 acgccggccg ggctgtatcc gaaggaccgg ctgatcgagc tcgaccgcga agggatcgac
      421 gccgcggtgc tgtacccgtc gctcgggctc gggatcggcg gcctcgtcga tccggagcac
      481 gcggtgctcg cgtgccggat ctacaacgac tggctcgcgg agtactgcgc cactgatccg
      541 gcgcgcctca tcgggatcgg cgcggtgccc atgcaggacc cgaaggaagc cccgcgtgaa
      601 gcggagcggt gcgtgcgcga gctggggttc cgcggcgtgt tcatccgacc gaacccgatc
      661 ctcgacatgt ggccgcacca ccgcgtgtac gacgagctgt ggtcgacggt cgagggcctc
      721 ggcgtgcccg tcggcttcca cccggccggc ttccccgaca ccttcggtgc cgcgcggacc
      781 tacggtccgg agtggcaggg gatcacaccg ctcggcaagg tgatcaactt catgatcgac
      841 gtcgtgaaca ccttcacgat gatgatcgcg gccgggacgc tcgaccgctg cccggagctg
      901 aagctcatcg tgctcgagtc gggctgcggt tggctgcact ggtggctcga caagatggat
      961 cactggaacg aggcgcgcct cgcgggcccc gggatggagc tcaagccgag cgagtacgtc
     1021 gagcgtcaga tctgggtgtc cggtgacccc gacgagagca gcttcgggtc ggtgtcggag
     1081 gtcgcgccgc cgggtcggct gatgtgggcg tcggacttcc cgcacctcga catcctcgag
     1141 agcgagccgt cgtgcaccga ggagctgtac gagcagctcg agtcgttgcc gcgagacgtt
     1201 gccgagggaa tccttggcta caacgcgtgt gacgtctacg gcctcgacgt gcgcgcgggc
     1261 ggctgatgca cgcgatcatc atcacgcgca tggtcgtgca tgccgaccag gtggatcgcg
     1321 ccagtgaggt cttccgcgcg atgcaagccg acgtgcgcgc gcaggagccg ggcacggtct
     1381 tctacgcgta ttaccaagcc gacgacgacc cgaccgtgtt ctgggtgcac gaggtgttcg
     1441 cccacgacgc cgcgaagcag ttccatctcg ggaaccacgc gcaccgccgc gccgacttcg
     1501 acgcgatcct cgcgcagccg cccgagttcc acatggtcca cgagctctga gtcgtgtcgc
     1561 gatgaggctc ggggtcgcgc tcggcaacct cggactcgcc attggacggc cgctcgaagg
     1621 tgccgagcac ggggcgctcg tctaccgcat cgccgaagcg gccgaggagc tcgggttcca
     1681 ttccgtctgg gccggcgacc acctcgcgct tccgcgcaca ccgaccacgc cctacccgta
     1741 cggtgacgat gccgcgctgc tctccgccga cgtgtcgctg ctcgatccgt tcgccgtcct
     1801 cgccgcggtg gcgggccgta ctgagcgcgt gcgcctcggt ttcggcgtcg ccgttctccc
     1861 gtaccggcat ccgctggtcg tcgcgaagct cgtcgcgagc atcgacgcgc tgtcgaacgg
     1921 ccgcgtgatc ctgggcgtcg gcaccggatg gatgcccgag gagttcgacg cggtcggcgc
     1981 cgacttcggc gcgcgcggtc gcgacaccga tgccgcgctc gcgtacctgc gtcgcgtgtt
     2041 cgccgacggt gaagccgacg acctgaccgt gctgccggtc cccgtgcaac gaccgggtcc
     2101 gccgatctgg gtcggcggcg tcgcgccggc cgcattgcgg cgcgcggtcg agttcgccga
     2161 cgggtggaac gcgccctacg ccgatccgga acggttggcg aacggaatcg agcggctcca
     2221 tgccgagtgc aggatcgcgg gccgcgacgc ggagtcgatc gacatctccg tacacggcat
     2281 cgccgccgac gacgtcgacg acgcgctcct cgacgcgtat gccgcgctcg gtgtcaccga
     2341 cctcggcgta cggctgccgc tggccgatgc gggttcgccc gtccacgcat tgcgtgacct
     2401 cgcgacgcgg tgcccgaggg tgcttgacca cattgggtga cgtccttact gtcgcaaccg
     2461 ggcaggaagg ccgacgaaaa cgagatcgat gaagcgtgtc ctcgcgagcg ttgccgtcgt
     2521 cgccctcgtc gcgacgtccc ttctcgcgac agcgggcgcg gctggggccg tggtgcggtc
     2581 ggcggccgat ctcgcggccg attgcaacga cgacggaatc gtgtcgatca caggcgacac
     2641 gacgtatgtg ggcggcaccg gcgagatcac gggtacacag ttgttcccag ggagtgcgcc
     2701 gatctgcatg atcgacgtcg aggcggcgac cgtgacgctc aagatca
//
LOCUS       DASUGA010000180         2103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_662749, whole genome shotgun sequence.
ACCESSION   DASUGA010000180 DASUGA010000000
VERSION     DASUGA010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662749"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="VFX21_03510"
     CDS             <1..913
                     /locus_tag="VFX21_03510"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX5095048.1"
                     /translation="VAPPLHDANLKLCHIETPIGAPDGEVLTGEVFSAPNELAAAIKD
                     AGYDGCSTASNHSFDAGEEGLRDTLDALDAQGVGHAGTARSADEDAAVTVYDVLGIHV
                     AHLAYTFGLDDAPLPAAESDMVSVIDPDKIGADARAAHAAGANIVVVSLHWGTDFTAE
                     PTVAQVELAEQLSHVPEITLVIGHHAHVVQPITKVGDLVVAYGLGNFLSDQHESECCP
                     AESQDGVIARFTFTETAPESGAFTVSDVAYTATRVDLATHTIVPVNTALANPGLDDDD
                     RDVLDASRKRTDEAIGMLGLLAHPTGT"
     gene            complement(957..1688)
                     /locus_tag="VFX21_03515"
     CDS             complement(957..1688)
                     /locus_tag="VFX21_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX5095049.1"
                     /translation="MRQDELAGSGQAVLVAIADERRPEAFARALARAAMMPALAFDDH
                     DVRAFMRSMPFAAVVVDGDVDPSGAATAPLAGRRDVVVCFLARDARRAAPDHAHAVFP
                     ADIAPAELASRVRALVGLRGDDASLLSWGPLQLDLRRRRAAFDGADLVTTPVQFRILA
                     ALARARGGVVTKEELQSEAWPWAAHDDGERLVAHIRRIRAKLEPYPSGAHFLVTARGE
                     GFRLADVAAAWDGVERRKADRRKGA"
BASE COUNT          353 a          757 c          695 g          298 t
ORIGIN      
        1 ggtcgcgccg ccgttgcacg acgcgaatct caagttgtgc cacatcgaga caccgatcgg
       61 cgcgcccgac ggcgaagtgc tgacgggcga ggtgttcagc gcgcccaacg agctcgcggc
      121 ggccatcaag gacgccggct acgacggttg ttcgacggca tcgaatcact cgttcgacgc
      181 cggggaagaa ggtctgcgcg acaccctcga cgccctcgac gcgcagggcg tgggccacgc
      241 cggtacggcg cggtcggccg acgaggacgc cgcggtgacc gtctacgacg tcctgggcat
      301 ccacgtcgcg cacctcgcgt acacgttcgg gctcgacgac gcgccactcc ccgccgcgga
      361 gagcgacatg gtcagtgtga tcgatcccga caagatcggc gccgatgcgc gcgccgcaca
      421 cgccgcgggc gcgaacatcg tcgtcgtaag cctccactgg ggtaccgact tcacggccga
      481 accgacggtc gcgcaggtcg agctggccga gcagctctcg cacgtgcccg agatcacact
      541 cgtcatcggc caccacgcgc acgtcgtcca accgatcacg aaggtcggcg acctcgtcgt
      601 cgcctacggc ctcggcaact tcctctccga ccagcacgag tccgagtgct gcccggccga
      661 atcccaggac ggcgtcatcg cgcgcttcac gttcaccgag accgcgccgg agtccggcgc
      721 gttcaccgtg agcgacgtcg cgtacacggc gactcgcgtc gacctcgcca cgcacacgat
      781 cgtgccggtc aacacggcgc tcgcgaatcc ggggctcgac gacgacgatc gcgacgtcct
      841 cgacgcatca cgcaagcgca ccgacgaagc aataggaatg ctggggttac tcgcccaccc
      901 cacgggcacc tagcggcacg gacgtcacgt gcgcactccc ggagcgacaa gaagatctac
      961 gcgccctttc ggcggtcggc tttgcggcgt tcgacgccgt cccacgcggc cgcgacgtcg
     1021 gcgaggcgga agccttcgcc gcgcgcggtc acgaggaagt gcgcgcccga gggatacggc
     1081 tcgagcttgg cccggatgcg gcggatgtgc gcgaccagcc gctcgccgtc gtcgtgcgcg
     1141 gcccacggcc atgcttccga ctgcagctct tccttcgtca cgacgccgcc gcgggcacgc
     1201 gcgagcgcgg cgaggatgcg gaactggacc ggcgtggtga cgaggtccgc gccgtcgaac
     1261 gccgcgcgcc ggcgacgaag atcgagctgc aacggtcccc acgacaagag cgacgcgtcg
     1321 tcaccccgca gcccgaccag ggcgcgcacc cgcgacgcca gctccgcggg agcgatgtcc
     1381 gcggggaaca ccgcgtgcgc gtgatcaggc gcggctcgcc gcgcgtcgcg cgcgaggaag
     1441 cacacaacga catcgcgccg ccccgcgagc ggtgcggtcg ccgcgccgct cggatcgacg
     1501 tcgccgtcga cgacgaccgc cgcgaacggc atcgatcgca tgaacgcgcg gacatcgtga
     1561 tcgtcgaacg cgagggccgg catcatcgcg gcgcgtgcga gggcgcgtgc gaacgcttcc
     1621 ggtcgacgct cgtctgcgat cgcgaccagt accgcctggc ccgagcccgc cagctcgtcc
     1681 tgccgcatcc cgatcctcgt ctcgtcgtcc ctggcgagtt ctgtgaacct acgacagctc
     1741 ctgttgcctc gtggggcgat gtacgccgaa tcgctaaaag cggcaaaaca caaatgtgcg
     1801 cgctcgtgaa tccgttctca aacgacctcg gaactgttcg gatgctgccg ggtcgctgtc
     1861 ctttcttcgc ggtgcccgtt ctgttggggt tggcgcggtg tctcccatgg aggcatgcac
     1921 gtgcaaaggg ccctttacat ggaggttcaa accagtggtt aggtcaaagc tcttgcgagc
     1981 tctcgccctg acggcaagtg ccgctctccc gctcggcgtg gcggcggcag cgggtgcgac
     2041 ggagtcccat ggcgacgacg acgcaaccgt cgacagcacc aacggcgcaa cgaacagcaa
     2101 tac
//
LOCUS       DASUGA010000181         2572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1965005, whole genome shotgun sequence.
ACCESSION   DASUGA010000181 DASUGA010000000
VERSION     DASUGA010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1965005"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..600
                     /locus_tag="VFX21_03520"
     CDS             202..600
                     /locus_tag="VFX21_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX5095050.1"
                     /translation="MATMPTKEQIEEFLSGPPDRPVVMLNMLRFKARADQETHPGLSG
                     AEAVMSYSKAMKEFVEARGGSFVLAATIDSQLIGDGGEGFEFVGIMRYPSRSKYIELA
                     GDPEVARTIGKHREAGLESQWLFAMTEVTE"
     gene            597..1073
                     /locus_tag="VFX21_03525"
     CDS             597..1073
                     /locus_tag="VFX21_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095051.1"
                     /translation="MTGVQDIPRAHTPPGGYGEQMPPPILRGCVDELPAGAPDLRGTW
                     RVVDGTADGAPLAADHPIRNHVERIEQAGARVVVTAGGVVHDMVADGTYENGVNDVMA
                     ADLTTPISVAASFENGALVLRPRDLPDVEVRRWRDGAHVMWQYHTLFTVRMQHTDD"
     gene            complement(1100..1978)
                     /locus_tag="VFX21_03530"
     CDS             complement(1100..1978)
                     /locus_tag="VFX21_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5095052.1"
                     /translation="MRGDREWTVGGAAALEMAAPAAVEALCAVVAGHATRLGAETIGA
                     IRSVTSGANGLPALGGPVTDAGALDAVVRAFAEQFSIDVSSIDDGQRAEMSAVLGENL
                     FDAVQAIYVADFVPRLYAALDAVFGTSAWSVPDAFRTDDTWSLIDAFAMEVARLSTLD
                     AVTTELVRLRGARQHQCRLCKSLRNVTALREGADDATLEAIDSYADSALAPKAKAALA
                     LTDAMIWTPARIDPVVIDSVRASLAPVEVVEVALDVTRNAANKIAVALGADAAAVADG
                     AVALFDVDAEGKFTYL"
     gene            2060..2278
                     /locus_tag="VFX21_03535"
     CDS             2060..2278
                     /locus_tag="VFX21_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095053.1"
                     /translation="MALERTRMLTTLGLRALADHARLMDTEPARRAIMTVNDFTANLV
                     DILLAIVDAPTTVSSKSKPRASTRKARR"
     gene            complement(2303..>2572)
                     /locus_tag="VFX21_03540"
     CDS             complement(2303..>2572)
                     /locus_tag="VFX21_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEX5095054.1"
                     /translation="MGTKHGAWCVGCCWALMAALFALGIMSVTWMAVVAVVIAVEKTL
                     PWRRAALIGTVALLSVLGVLVLCAPSTIPHLTTPTDHPMQQMSNM"
BASE COUNT          448 a          919 c          840 g          365 t
ORIGIN      
        1 cacggcaact tcgtccgcgc ctggcacaag gcgaccctgc agtcgttctt cgccggctac
       61 ctgcgcccgg aggacgcact gcgcctcctc gtgaccggcg cgcacggcaa catcccgtag
      121 gtcgacacct cggtgtgaca ccggggacgg aactccacct acggtacgag ccacgcgccc
      181 gtcactgcgc aggaggagcc catggcgaca atgccgacga aggagcagat cgaggagttc
      241 ctctcggggc cacccgacag gcccgtcgtg atgctgaaca tgctgcgctt caaagcccgc
      301 gccgaccagg agacccaccc cggtctcagc ggcgccgagg ccgtgatgtc gtactcgaag
      361 gccatgaagg agttcgtcga ggcgcgcggc ggatcgttcg tgctcgcggc gacgatcgat
      421 tcgcaactca tcggcgacgg cggcgaggga ttcgagttcg tcgggatcat gcgctacccg
      481 tcgcggagca aatacatcga gcttgccggt gatccggaag tggcgcgtac gatcggcaag
      541 catcgcgagg cagggctgga gagccagtgg ctcttcgcga tgacggaggt gaccgagtga
      601 ccggcgtcca agacatcccc cgcgcgcaca cgccacccgg cggctacggc gaacagatgc
      661 cgccgccgat cctccgcggg tgtgtcgacg agcttccggc cggcgcgccc gacctgcgcg
      721 ggacctggcg ggtcgtcgac ggcactgcgg acggtgcgcc ccttgcggcc gaccatccga
      781 tccggaacca cgtcgaacgc atcgaacagg ccggcgcgcg cgtggtcgtc accgcgggcg
      841 gcgtcgtcca cgacatggtc gccgacggca cctacgagaa cggcgtcaac gacgtgatgg
      901 ccgccgacct cacgacgccg atcagcgtcg cggcatcgtt cgagaacggc gcgctcgtgt
      961 tgcggccgcg cgacctcccc gacgtggagg tacgcaggtg gcgcgacggt gcgcacgtca
     1021 tgtggcaata ccacacgctg ttcacggtgc gcatgcagca caccgacgac tgatcggctt
     1081 ctcgctcaga ggttctcgct cagaggtacg tgaacttgcc ttcggcgtcg acgtcgaaca
     1141 gcgcgacagc gccgtcggcg accgcagccg catccgcccc gagcgcgacg gcgatcttgt
     1201 tggcggcgtt gcgcgtcacg tcgagcgcga cctcgacgac ctcgaccggc gccaggctcg
     1261 cgcgaaccga gtcgatgaca actggatcga tgcgagcggg agtccagatc atcgcatccg
     1321 tcagcgcgag cgcagccttc gccttgggtg cgagcgcgct gtccgcgtag gagtcgatgg
     1381 cctcgagcgt ggcgtcgtcg gcgccctctc gaagagcggt cacatttcga agcgacttgc
     1441 acaaccgaca ctgatgctga cgcgcaccgc gcagccgcac caactcggtc gtgaccgcgt
     1501 cgagcgtgct cagccgagcg acctccatcg cgaacgcatc gatcaggctc cacgtgtcat
     1561 cggtgcggaa cgcgtccggc accgaccagg cgctcgtccc gaacacggcg tcgagagccg
     1621 cgtagagccg cggcacgaag tcagcgacgt agatcgcctg gaccgcgtcg aacaagttct
     1681 cgccgagcac ggcgctcatc tcggcccgct gtccatcatc gatcgacgag acgtcgatgc
     1741 tgaactgttc cgcgaacgcg cggaccacgg catcgagcgc gccggcgtcg gtgacgggac
     1801 cgccgagcgc cggcagcccg tttgctcccg acgtcacgct ccgtatcgcg ccgatggtct
     1861 cggcgccgag gcgcgtcgcg tgaccggcga cgaccgcgca cagcgcctcg acggctgccg
     1921 gcgcggccat ctccagggcc gcggcgccgc cgaccgtcca ctcgcgatcg cctctcaccg
     1981 gcgggatcct agaggatcgc ttcgtctcgc cgatcgtgac gacggcactg gtcgacgcgt
     2041 cgcgaatctt ccccgcgccg tggcactcga gcgcacgagg atgctgacca cactcggcct
     2101 gcgtgcgctc gccgaccatg cccgcctcat ggacaccgaa ccagcacggc gcgcgatcat
     2161 gacggtcaac gacttcaccg ccaacctggt cgacatcctg ctcgcgatcg tcgatgcacc
     2221 cacgaccgtg agctcgaaga gcaagccccg agcgtcgacg cgcaaagcgc gccgatgagt
     2281 cccgagcgcg acgatcccgc gtctacatgt tcgacatctg ctgcatcggg tggtccgtcg
     2341 gggtcgtgag gtggggaatc gtcgacggcg cgcacaggac cagcacgccg aggacgctta
     2401 ggagcgccac ggtgccgatc aacgcggccc gacgccacgg caacgtcttc tcgacggcaa
     2461 tgacgacggc aacgacggcc atccacgtca cgctcatgat gcccagcgcg aacagcgcgg
     2521 ccatcagcgc ccaacagcag ccgacacacc aggcaccgtg tttcgtcccc at
//
LOCUS       DASUGA010000182         2747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_175582, whole genome shotgun sequence.
ACCESSION   DASUGA010000182 DASUGA010000000
VERSION     DASUGA010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175582"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VFX21_03545"
     CDS             <1..739
                     /locus_tag="VFX21_03545"
                     /inference="COORDINATES: protein motif:HMM:NF020926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Zn-binding domain-containing protein"
                     /protein_id="HEX5095055.1"
                     /translation="DARVFSVAHPGAIYLHQGRQYRVASLDLRSHVAHVLADDDADEY
                     TQPRTETDIAIVDEDRSAPLGATTAHLGAVEVSNQVVAYQRKRISTNDVLDVVPLDLP
                     ERSLVTRACWYTVPAEVTEGAGITPAQLIGAVHAAEHGLIGMLPLFTICDRWDVGGVS
                     MALHPQTGEPTIFIYDGYPGGAGIAELAFEAAARHAAATLDLVAECPCEDGCPSCVQS
                     PKCGNWNEYLDKGAATSLLRLMATPRG"
     gene            complement(717..1067)
                     /locus_tag="VFX21_03550"
     CDS             complement(717..1067)
                     /locus_tag="VFX21_03550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3654c family TadE-like protein"
                     /protein_id="HEX5095056.1"
                     /translation="MSMVVLGAFALCVALAIGIGRLGAAAVLDARAQTAADAAALAAA
                     DQLALGHGSKAANQAAAATTRANGATLVQCDCGGEAAEVEVELTGSYAALGLPHAAHA
                     KARAEVDLSRAVSP"
     gene            complement(1085..1477)
                     /locus_tag="VFX21_03555"
     CDS             complement(1085..1477)
                     /locus_tag="VFX21_03555"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX5095057.1"
                     /translation="MIGGSGWRGARHARSASGQATVEFALVLPVVLLLILALFQMALI
                     ARDQVLATHSARVAAREASVGAPGNRVQAAAESVLDDVDVDVDKGAKVGDDVTVTVHY
                     TSHTSLPLVGALFPDVDMEATATMRREK"
     gene            complement(1477..1677)
                     /locus_tag="VFX21_03560"
     CDS             complement(1477..1677)
                     /locus_tag="VFX21_03560"
                     /inference="COORDINATES: protein motif:HMM:NF025395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
                     /protein_id="HEX5095058.1"
                     /translation="MTEVFTRIWVAVCNHFERDERGQTTAEYALVLLAAAALGMLLLT
                     WAKGSHAVSSLFDKVINQILPG"
     gene            complement(1704..2366)
                     /locus_tag="VFX21_03565"
     CDS             complement(1704..2366)
                     /locus_tag="VFX21_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5095059.1"
                     /translation="MIVWVIALAWAAVAMYGVLALADHRAVVRRTRAVRVRSRRRPRR
                     RRRSVPLPRAVSHWWWVLGGPFRRRRRRRHEDRLRSELPIGVDLVAVAVGAGCTPFQA
                     VELAQRWSPKLVAGVLHGIARATALGASFDDALRDTGSGTPIVRPLTDVLRTSAALGS
                     PASASLGRLALDVRADVRRRAEAHARTIPVRLLFPLVFCILPAFALLTVVPLFAGGLP
                     IG"
     gene            complement(2363..>2747)
                     /locus_tag="VFX21_03570"
     CDS             complement(2363..>2747)
                     /locus_tag="VFX21_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095060.1"
                     /translation="TMTDALGVWASTRTTSSAHAAAGALSLSSAVGGRAADALEGLAT
                     SLRERLAIMAETRALSAQARYSAFVVGLGPLAFIAVSTMLDRGAASALVGTSVGRVCA
                     VVGIVLELFGALWMRRIVRSAEAFA"
BASE COUNT          445 a         1001 c          955 g          346 t
ORIGIN      
        1 cgacgcgcgc gtgttctcgg tcgcgcatcc gggtgcgatc tacctgcatc agggtcgcca
       61 gtaccgcgtg gcgagcctcg atctccgcag tcatgtcgca cacgtgctgg ccgacgacga
      121 cgccgacgag tacacgcagc cgcggaccga gaccgacatt gcgatcgtcg acgaagaccg
      181 gagcgcgccg ctcggtgcga ccactgcaca tctgggcgcg gtcgaagtgt cgaatcaggt
      241 cgtcgcctac caacgcaagc ggatctcgac caacgacgtg ctcgacgtgg tgccgctcga
      301 cctccccgag cgctcgctcg tcacgcgcgc gtgctggtac acggtgccgg ccgaggtgac
      361 ggagggcgcc ggcatcacgc ccgcgcagtt gatcggcgcc gtgcacgcgg ccgagcacgg
      421 gctgatcggc atgctgccgc tgttcacgat ctgcgatcgg tgggacgtcg gcggcgtgtc
      481 gatggcgctg cacccgcaga cgggcgaacc gacgatcttc atctacgacg gctaccccgg
      541 cggcgcgggg atcgccgagc tcgcgttcga ggccgcggcg cgccatgcgg cggcaacgct
      601 cgatctcgtt gccgagtgcc cgtgcgaaga cggctgcccg tcgtgtgtgc agtcgccgaa
      661 gtgcggcaac tggaacgagt acctcgacaa aggtgccgcg acctcattgt tgcgactcat
      721 ggcgacaccg cgcggctgag gtcgacttcc gctctcgcct tggcgtgcgc ggcgtgcggg
      781 aggccgagtg cggcgtacga gccggtgagc tcgacctcga cttccgcggc ctcaccgccg
      841 cagtcgcact gcacgagcgt ggcgccgttg gcgcgcgtcg tcgcggccgc ggcctggttc
      901 gccgccttcg agccgtggcc gagcgcgagc tggtcggcgg ccgcgagggc ggcggcgtcg
      961 gctgccgtct gcgcccgcgc gtcgaggacc gcggcggcgc cgagacgacc gatgccgatc
     1021 gccagtgcga cgcacagggc gaacgcaccg agcacgacca tgctcaccga gcccgattct
     1081 cggttcactt ctcgcgccgc atcgtcgctg tggcttccat gtcgacgtcg gggaagaggg
     1141 cgccgacgag cggcagcgac gtgtgtgacg tgtagtgcac ggtgacggtc acgtcgtcgc
     1201 cgaccttcgc gcccttgtcg acgtcgacat cgacatcgtc gagcaccgac tcggccgcgg
     1261 cctgcacgcg attgcccggc gcgcccacgc tcgcttcgcg cgccgcgacg cgcgccgagt
     1321 gcgtggcgag cacctggtcg cgcgcgatga gcgccatctg gaacaacgcc aggatcaaca
     1381 gcaagacgac gggcaggacg agcgcgaact cgacggtcgc ctgcccggag gcggaacgcg
     1441 cgtgacgcgc gccccgccaa ccggaaccgc cgatcatcag ccgggcagga tctggttgat
     1501 gaccttgtcg aagaggctcg acaccgcgtg tgaacccttg gcccaggtca acagcagcat
     1561 gccgagcgcg gccgccgcga gcaggacgag cgcgtactcc gcggtcgtct gcccgcgttc
     1621 gtcgcgctcg aagtggttgc acactgcaac ccagatcctg gtgaagactt cggtcacttc
     1681 gttctccttg gtgaaagacg cgttcacccg atgggaagcc cgccggcaaa gagcggcacg
     1741 accgtcagca gcgcgaacgc gggaaggatg cagaacacaa gggggaagag caggcgcacg
     1801 ggaatcgtgc gcgcgtgggc ctcggcccgg cgccgcacgt cggcgcggac atcgagcgcg
     1861 aggcgaccga gcgacgccga cgcgggcgag cccaacgccg cgctcgtccg gagcacgtcg
     1921 gtgagcggcc gcacgatcgg cgtgccggac ccggtgtctc gcagcgcatc gtcgaacgac
     1981 gcaccgagcg ccgtcgcacg cgcaataccg tgcagcacgc cggcgacgag cttcggactc
     2041 caccgctgcg ccagctcgac cgcctggaac ggcgtacagc cggcgccgac cgccacggca
     2101 acgaggtcga cgccgatcgg cagctccgaa cgcagccggt cttcgtgccg gcgccgtcgc
     2161 cgccgccgga acggaccacc gagcacccac caccagtgcg agactgcccg cgggagcggc
     2221 accgagcgcc ggcggcggcg cgggcgacgc cggctgcgca cccgcacggc ccgcgtgcgc
     2281 cgcacgacgg cccgatgatc ggccaacgcg agcacgccgt acatcgcgac ggccgcccac
     2341 gcgagcgcga tcacccacac gatcatgcga aggcctccgc gctgcgcacg atgcggcgca
     2401 tccacaacgc gccgaagagc tccagcacaa tgccgacgac cgcacacacg cgtccgacgg
     2461 atgtaccgac gagcgcgctt gcggcaccgc ggtcgagcat cgtcgacacc gcgatgaatg
     2521 ccagcggacc gagcccgacg acgaacgccg agtagcgcgc ctgcgccgac agcgcccgcg
     2581 tctcggccat gatcgccagg cgttcccgca acgacgtcgc gagcccttcg agcgcgtcgg
     2641 ccgcacggcc gccgacggcg ctcgacaacg agagcgcgcc cgccgcagca tgtgcgctcg
     2701 atgtcgtgcg cgtcgatgcc cacacgccga gcgcatccgt catcgtc
//
LOCUS       DASUGA010000183         2615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1147923, whole genome shotgun sequence.
ACCESSION   DASUGA010000183 DASUGA010000000
VERSION     DASUGA010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147923"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /gene="aspS"
                     /locus_tag="VFX21_03575"
     CDS             complement(<1..1307)
                     /gene="aspS"
                     /locus_tag="VFX21_03575"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX5095061.1"
                     /translation="MRKTDVGTSVALCGWVANPRDHGGKIFIDLRDVAGIVQVVVDPA
                     LPGLDVGHRLRREWVVRVIGDVVARPAESVNPELPTGEVEVVAREVEVLNEAEPPPFQ
                     LDNRGDVDETLRLRHRYLDLRRPEMQRNLRLRAAMNAAMRTAMDEQGFVEVETPMLIA
                     STPEGARDFVVPSRLHPGAFYALPQSPQLFKQLLMVGGVDRYYQIARCLRDEDLRADR
                     QFEFMQLDIEMTFADQEDVLDAISTAVLRATEAVRPGEAPASIPRMTWHDAMERYGSD
                     KPDVRFGMELVDLDEVFAVTEFRAFAGADCIKGINVEGQGDLSRSKLDALTERAKSLG
                     AAGLVWMRVGDGGALESPVAKFLSEVEMIGLVDAMGAKAGDLLLLVAGDRPMARSVLG
                     TLRLDLGRPPISEGGLAFLWVVDFPLFEGTSEGGTVIPMHHPFT"
     gene            complement(1331..2599)
                     /gene="hisS"
                     /locus_tag="VFX21_03580"
     CDS             complement(1331..2599)
                     /gene="hisS"
                     /locus_tag="VFX21_03580"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020547130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX5095062.1"
                     /translation="MADWELLRAPKGMLDVLPPESGRWIDLVAQFATRAHRFGYGLLL
                     TPMVEHYEVFARVGASTDVVRKEMYDFVDRGGRRLALRPEGTAPVVRAYVEHRPPTPW
                     KVWYLAPNFRAERPQAGRYRQHWQLGVEVIGVDDPDVDVEVIELLWGFYRELGLRDVQ
                     LLLNSMGDQESRGRYRTVLLEYWRAHAELIGDEMERAETNPLRILDSKRPDWQEMLER
                     APQLGEYLSTASEAHFARVQEGLQALGIAYEIAPRLVRGFDYYTSTVFEFASGALDSA
                     QNAVGGGGRYDRLSEEMGGPAAPSIGFGSGIERLLLACDAEGVLPAPEARVDAFVVDL
                     LGGNDPLRLLAELREAGLAADRSYGSRKPKQQFAAADKSGAAWAVVLGAKEAERGLVS
                     IKDLRSGEQIEVRREEVAAWLCTRKDEISP"
BASE COUNT          442 a          947 c          823 g          403 t
ORIGIN      
        1 gtgaacgggt ggtgcatcgg gatgacggtg ccgccctcgc tcgtgccctc gaacaacggg
       61 aagtcaacaa cccaaagaaa tgcgagtccg ccttcggaga tcggtggacg gccgaggtcg
      121 agccgaagcg tgccgagcac ggaccgcgcc atcggccggt cgccggcaac gagcagcaag
      181 aggtcgcccg ccttcgctcc catcgcgtcg acgaggccga tcatctccac ttccgacaag
      241 aacttggcga ccggcgactc caacgctccg ccgtcgccga cgcgcatcca cacgagcccg
      301 gccgcgccca gcgacttggc gcgctcggtc agcgcgtcga gcttcgaacg cgacagatcg
      361 ccttggccct cgacgttgat ccctttgatg cagtcggcac cggcgaacgc gcgaaactcg
      421 gtcaccgcga agacctcgtc gaggtcgacc agttccatgc cgaaacgcac gtcgggcttg
      481 tcggagccgt aacgctccat cgcgtcgtgc catgtcatgc gcgggatcga tgccggcgct
      541 tcaccgggtc ggacggcctc ggtcgcgcgc agcaccgcag tcgagatggc gtcgagcacg
      601 tcttcctggt cggcgaacgt catctcgatg tcgagctgca tgaactcgaa ctggcggtcg
      661 gcgcgcaggt cctcgtcgcg caggcaccgc gcgatctggt agtagcggtc gacaccgccc
      721 accatcaaca actgcttgaa caactgcggc gactgcggaa gcgcgtagaa ggcgcccggg
      781 tgcaatcgcg acggaacgac aaagtcgcgc gcgccctccg gcgtcgaggc gatcagcatc
      841 ggcgtctcga cttcgacgaa cccctgctcg tccatcgcgg tccgcatcgc ggcgttcatc
      901 gccgcgcgca gccggaggtt gcgctgcatt tcggggcgcc gcagatcgag atagcgatga
      961 cgcagccgca aggtttcgtc gacgtcgccc cgattgtcga gctggaacgg cggcggctcc
     1021 gcctcgttca gcacctcgac ctcgcgtgca accacttcga cctcaccagt cgggagttcg
     1081 ggattcacgc tctcagccgg gcgcgcgacg acgtcaccga tcacacgcac gacccattcc
     1141 cgccgcagac ggtgccccac gtcgagaccg gggagcgccg ggtcgacgac cacctggacg
     1201 atgccggcga cgtcgcgcaa gtcgatgaag atcttcccgc cgtgatcgcg cggattcgct
     1261 acccagccgc acaacgcgac cgacgtgcct acgtcggtct tccgcaagtc gccggctcga
     1321 tgcgatcgca tcatggggag atctcgtcct tcctcgtgca caaccacgcc gcgacctctt
     1381 cgcgacgcac ttcgatctgt tcaccggaac gcaggtcttt gatcgagacc aatccgcgtt
     1441 ctgcttcctt cgcgcccagc acgaccgccc acgcggctcc cgacttatcg gcggccgcga
     1501 actgttgctt cggcttgcgg ctcccgtagg aacggtcggc cgccaatccc gcttcacgca
     1561 gttcggcgag caggcgcaac gggtcgttcc cacccaggag gtcgacgacg aacgcgtcga
     1621 cgcgcgcctc cggcgcaggg aggacgcctt cggcgtcgca cgccagcagc aagcgctcga
     1681 tgcccgaccc gaagccgatc gacggcgcgg ccgggccacc catctcttcc gacagccggt
     1741 cgtagcgacc gcccccgccg acggcgttct gcgcactgtc gagtgcgccg ctcgcgaact
     1801 cgaacaccgt cgaggtgtag tagtcgaacc cgcggacgag ccgcggcgcg atctcgtagg
     1861 cgatgccgag cgcctgcagc ccttcctgca cccgcgcgaa atgcgcctcc gacgcagtcg
     1921 agaggtactc ccccagttgc ggcgcccgct cgagcatttc ctgccagtcg gggcgcttcg
     1981 agtcgagaat gcgcaacggg ttcgtctccg cgcgttccat ctcgtcgccg atcagctccg
     2041 cgtgcgcgcg ccagtactcg agcaggacgg tgcgatagcg gccccggctc tcctggtcgc
     2101 ccatggagtt cagcaacagc tgcacatcgc gcagccccag ttcgcgatag aagccccaca
     2161 gcagctcgat gacttcgacg tcgacatccg gatcgtcgac gccgatgact tcgacgccga
     2221 gctgccagtg ctggcgatag cgcccggcct gcgggcgttc ggcccggaag tttggggcga
     2281 ggtaccaaac cttccacggt gtcggcggcc ggtgctcgac atacgcgcgc acgacgggcg
     2341 cggtgccctc cggccgcagc gccaggcgcc gcccgccccg gtcgacgaag tcgtacatct
     2401 ccttgcgcac gacgtcggtc gaggcgccga cgcgcgcgaa cacttcgtag tgttcgacca
     2461 tcggggtcag cagcaagccg tacccgaagc ggtgcgcccg agtcgcaaac tgcgcaacaa
     2521 ggtcgatcca gcgccctgat tccgggggca ggacgtcgag catccccttt ggtgcgcgca
     2581 gcagctccca gtcggccatc cgcgcagact accga
//
LOCUS       DASUGA010000184         3614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1183054, whole genome shotgun sequence.
ACCESSION   DASUGA010000184 DASUGA010000000
VERSION     DASUGA010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183054"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VFX21_03585"
     CDS             complement(<1..746)
                     /locus_tag="VFX21_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095063.1"
                     /translation="MLGRTWRIGRYVATATLIAGGLIAMPATAPAISLRAGTSAPIIG
                     LAATPSGGGYWQVGLDGGVVNSGDAGFYGSMTGTPLNKPIVGMAPTTSGRGYWLTASD
                     GGMFNFGDARFYGSTGAMRLNQPIVGMAATPTGAGYWLVASDGGIFSFGDARFYGSTG
                     GLRLNRPIVGMAATPSGRGYWLVASDGGVFGFGDASFYGSTGGMVLNKPIVGIAPAID
                     GRGYLMVASDGGVFKFGSVEFFGSAASACQT"
     gene            complement(855..2828)
                     /locus_tag="VFX21_03590"
     CDS             complement(855..2828)
                     /locus_tag="VFX21_03590"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEX5095064.1"
                     /translation="MRTADETAARPRPGTRSARSRPSARKKSKRTRRPVWQLVAMVAG
                     GALLVVFFLAVAERVVYSGKVMPGVDVEGVDVAGQSEDDSYADISALASDLETQPLHA
                     TIDGREVVADASLLEVDVDEAATLRDARKAARSGNPIEQTIGAVLRRFRPDDVSLHVS
                     YNEAGLAGVIDAWERETLDGRVEGNIEFDGTKVVEVLPKAGTGLQRDEARELLDEELH
                     RAERKTVKLPVGKVQPKLTRDDVATAAAQARAILGKTHDLATDGATITITPEQLVSAM
                     GTTARESAAGGPGKARADADASLELTIDPEKLRAALGDSLGALETAPVDATFEVTSSG
                     GVNVVPSQNGRQVDMTMVADALLEDQTHITAALEETPPEHDTAWAESLHIKEQVSTFT
                     TNHRAGEERVKNIHRAADLLNNTIVEPGATFSLNDTIGPRTEERGFVVAPVFYGEFTE
                     DFGGGVSQLATTTFTAVFWGGYEDVYHKPHTIYITRYPMGREATVNYPTVDLKFKNNT
                     SAGVLIRTSYSSSSITVTFYGDKEGKTVNEEDRKVLGETPVGDLYYDCPAPANVDPNN
                     VCANLPEGQSKRVEEGHAGADVEFYRVIEQPGHDTTRERFFWRYRKTDNKTVIGHAAA
                     PTTTVAPGATTATSTPTVAETTVPPPTVPAPNP"
     gene            2905..>3614
                     /locus_tag="VFX21_03595"
     CDS             2905..>3614
                     /locus_tag="VFX21_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095065.1"
                     /translation="MHEHLRSLLRELGASDEEMDRAEAQGWLPLLAVDRVLMPGQPRY
                     DLEAAAAQAGVDVEFARRLWRTMGFPDSPQGVAAFTDRDVEALRLAAERLRPGDDVHL
                     FLRQSRAIGAVMARLGAIDADLIARALDEMRAEGLDDETIAETVMLGIDWPSLATLID
                     HVHRVQARAAVWRRLARDAYADVGLGIGFCDLAGYTQLSAELDPDALSELLTTWEDVS
                     YDIVAQLGGRVVKSIGDEV"
BASE COUNT          525 a         1196 c         1265 g          628 t
ORIGIN      
        1 gtctggcacg cgctcgccgc ggaaccgaag aactcgaccg atccgaactt gaagacgccg
       61 ccgtcggacg caaccatcaa gtagccgcgg ccgtcgatcg ccggcgcgat gccgacgatg
      121 ggcttgttga gcaccatgcc gcccgtcgaa ccgtagaacg acgcgtcacc gaacccgaag
      181 acgccgccgt cgctggcgac cagccagtag ccgcgccctg acggcgtcgc ggccatgccg
      241 acgatgggcc ggttcaggcg gaggccgccc gtcgatccgt agaagcgcgc gtcaccgaag
      301 ctgaagatgc cgccgtcgct ggcgacgagc cagtatccgg cgccggttgg tgtcgcggcc
      361 atgccgacga tcggctggtt gagacgcatc gcgccggtcg atccgtagaa gcgcgcgtca
      421 ccgaagttga acatgccgcc gtcgctcgcc gtgagccagt agccgcgacc ggatgtcgtc
      481 ggcgccatgc cgacgatcgg tttgttcaac ggcgtgccgg tcatcgagcc gtagaagccc
      541 gcgtcacccg agttgacgac gccgccgtcg agtccgacct gccagtagcc gccgcccgac
      601 ggtgtcgcgg cgaggccgat gatcggggcg ctcgtgcccg cgcgcaatga gatggcaggc
      661 gcggtcgcgg gcatcgcgat cagaccaccg gcaatgagtg ttgcggtagc gacgtaacgt
      721 ccgatgcgcc acgtccgacc cagcaccaac gccacctcgt ccatccccct tgccgcacca
      781 ccggaaatgt gcggctccga ttgggatcgg cagcgggctt ggggatgttg aggaattcag
      841 acggtcaggc tgaatcaggg attcggtgcg ggcactgtgg gtggcggaac cgtcgtttcg
      901 gccacggtgg gcgtcgacgt ggctgtcgtc gcacccggtg cgaccgtcgt cgtcggcgcg
      961 gccgcatggc cgatcacggt cttgttgtcg gtcttgcggt accgccagaa gaagcgttcg
     1021 cgcgtcgtgt cgtgccccgg ttgttcgatc acgcggtaga actcgacgtc ggcgcccgcg
     1081 tgcccttctt cgacacgctt gctctgacct tcgggcaagt tcgcgcacac gttgttcggg
     1141 tcgacgttcg cgggtgcggg gcagtcgtag tagaggtcgc cgaccggcgt ctcgccaagg
     1201 actttgcggt cctcttcgtt cacggtcttg ccttctttgt cgccgtagaa cgtgacggtg
     1261 atcgacgacg aggaatacga agtgcggatc aacacgcccg cgctggtgtt gttcttgaac
     1321 ttcaagtcga ccgtcgggta gttgacggtc gcctcgcgtc ccatcggata acgcgtgatg
     1381 tagatcgtgt ggggcttgtg gtacacgtct tcgtagccgc cccagaacac cgcggtgaac
     1441 gtcgtcgttg cgagctggct cacgccgcca ccgaagtctt cggtgaactc gccgtagaac
     1501 accggtgcga cgacgaagcc gcgctcttcg gtgcgcggac cgatcgtgtc attcaacgag
     1561 aacgtcgcgc cgggctcgac gatcgtgttg ttgagcaggt ccgcggcgcg gtggatgttc
     1621 ttgacccgtt cttcgccggc cctgtggttc gtcgtgaacg tcgagacctg ttccttgatg
     1681 tgcagcgact cggcccaggc cgtgtcgtgc tcgggcggtg tctcttcgag cgcggcggtg
     1741 atgtgcgtct ggtcttcgag gagcgcgtcg gcgaccatcg tcatgtcgac ctggcgtccg
     1801 ttctgcgacg ggacgacatt cacgccgccc gacgacgtca cttcgaacgt cgcgtcgacg
     1861 ggtgccgtct cgagcgcacc gagcgaatcg ccgagcgccg cccgcagctt ctcgggatcg
     1921 atcgtcagct cgagtgacgc gtccgcgtcg gcgcgcgcct tccccggacc gccggccgcc
     1981 gattcgcgtg ccgtcgtgcc catcgcgctc acgagctgct cgggcgtgat cgtgatcgtc
     2041 gcgccatcgg tggcgaggtc gtgggtcttg ccgaggatcg cccgcgcctg cgcggccgcc
     2101 gtggcgacgt cgtcgcgcgt gagcttcggt tggaccttgc cgacgggcag cttcacggtc
     2161 ttccgctccg cccggtgcag ctcttcgtcg agcagttcgc gagcctcgtc gcgctggagc
     2221 ccggtgccgg ctttgggcag cacctcgacg accttggtgc cgtcgaactc gatgttgccc
     2281 tcgacccgtc cgtcgagcgt ctcgcgttcc cacgcgtcga tgacgccggc gagaccggcc
     2341 tcgttgtagc tgacatgcag cgagacgtcg tcggggcgga agcggcgcaa cacggcaccg
     2401 atcgtctgct cgatcggatt gccgctccgt gccgccttgc gcgcgtcgcg caacgtggcg
     2461 gcctcgtcga cgtcgacctc gagcagcgac gcatcggcca cgacctcgcg gccgtcgatg
     2521 gtcgcgtgca acggctgggt ctcgaggtcg ctcgcgagcg ccgagatgtc ggcgtagctg
     2581 tcgtcttcgc tctggccggc gacgtcgaca ccctcgacgt cgacgcccgg catcaccttg
     2641 cccgagtaca cgacgcgttc ggcgaccgcg aggaagaaca cgacgaggag cgcaccgccc
     2701 gcgaccatcg cgacgagctg ccacaccggg cggcgggtgc gtttcgactt cttgcgcgcc
     2761 gaggggcggg aacgagccga gcgagtgccg ggccgggggc gcgcggcagt ctcgtcggca
     2821 gttcgcatag acgggcaggc taccgaagcc gatcgcgcga tctgggggcg acggcggcat
     2881 ccgtacgcga cagaatcacg caccgtgcac gaacacctac ggtcgctgtt gcgcgagctc
     2941 ggcgcgagcg acgaagagat ggatcgcgcc gaagcacagg gctggctgcc gctgctcgcg
     3001 gtcgaccggg tgctcatgcc tggtcaaccg cgttacgacc tcgaggccgc ggccgcgcag
     3061 gccggcgtcg acgtcgaatt cgcgcgtcgg ttgtggcgga cgatggggtt cccggattca
     3121 ccccaggggg tggcggcatt caccgaccgt gacgtcgagg cgttgcggct ggcggccgaa
     3181 cggctgcgac ccggcgacga cgtgcatctg ttcttgcgcc agtcgcgcgc catcggcgcg
     3241 gtcatggccc gcctcggtgc gattgacgcc gatctcatcg cgcgtgcgct cgacgagatg
     3301 cgggccgagg gactcgacga cgaaacgatc gccgagacgg tcatgctcgg catcgactgg
     3361 ccgagcctgg ccacgctgat cgaccacgtg caccgggtcc aggcccgcgc cgcggtatgg
     3421 cggcggctgg cgcgtgacgc gtacgcggac gttggcctcg ggatcgggtt ctgcgatctc
     3481 gcgggctaca cgcagttgag tgccgagctc gatccggacg cgctgtcgga gctgctgact
     3541 acatgggagg acgtctccta cgacatcgtt gcgcagctcg ggggccgcgt cgtgaagtcg
     3601 atcggcgacg aggt
//
LOCUS       DASUGA010000185         3001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1668469, whole genome shotgun sequence.
ACCESSION   DASUGA010000185 DASUGA010000000
VERSION     DASUGA010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668469"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..724
                     /locus_tag="VFX21_03600"
     CDS             83..724
                     /locus_tag="VFX21_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016863827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5095066.1"
                     /translation="MDYTLTDRTQVRRLPARGRYDRALVHEIADEALTCSVGFAIDGK
                     PWVIPTIHARVDDTLYLHGAPANHMLRSLASGVETCVTITLVDGLVLARSAFHHSMNY
                     RSVVAFGVATKVEAFDEKRTALAALVEHVVRGRGIDARPPTDEELRATLVLRLPLEEV
                     SAKVRTGGPIDDEDDLGLPVWAGVLPCTTTYGTPEPDAGVDAAVPGYVTNYTR"
     gene            complement(721..2250)
                     /locus_tag="VFX21_03605"
     CDS             complement(721..2250)
                     /locus_tag="VFX21_03605"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5095067.1"
                     /translation="MPPQNVLLITTDQHRADTLGSTGNPLGATPRLDALAAEGTRYDG
                     ARTQNPLCQPARATILTGTYPSTHGVTCNGVDLPGDAEGRSIATLLANAGYATALIGK
                     AHFATAYPFQPTGKIESVEGSARVDPQWQGPYFGFEHAELMLFGHNLRIADLMGQWNW
                     IYGPPPFGLHYGQYLFRDGFERGAERLRLMQPEAVGEQWDDTQTWRNALPEEDHCTTW
                     TADRACAWLRDVDGPFFGWISFTDPHHPMDAPAPWCDRYQPEDVLEILFPVRDGEFTT
                     KPRLHEALAEGVRQPGMHWANPGGKNYTREQMAKLTAGYYGMVAQLDHAIGRVLDTLA
                     ARGLADNTLVIVTSDHGEFLGEHQMIFKGPFGYDSLLRVPLILRGPGVGAGEVVDDPV
                     GTIDLAPTMLRAAGVDVPSWMEGRALDGTPREHVLTENDFNIMVSLPMRTITTTRYKL
                     HHYLEAPWGELYDLQEDPGEIVNRYDDPAYASVRSELTDLLKDVMNHEVRKEPVVGLV
                     A"
     gene            2269..>3001
                     /locus_tag="VFX21_03610"
     CDS             2269..>3001
                     /locus_tag="VFX21_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018890524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HEX5095068.1"
                     /translation="METLRTPDERFASLPGYPFAPHYVTVSDDEGGELRVHYVDEGPA
                     DGAVVLLMHGEPSWAYLYRKMIPVLTAAGYRCVAPDLVGFGRSDKPTKRSDYTYARHV
                     EWMRSTLFDRLDLRDVTLVCQDWGGLLGLRLVGEHPDRFARVVTANTFLPTGDTPPGD
                     AFFNWQKFSQTVEDFDIGFIINMGSATDLSAEVIAAYNAPFPDDSYKEGARQFPMLVP
                     SRPDDPASPANRAAWETLRTFDKPWL"
BASE COUNT          474 a         1016 c          995 g          516 t
ORIGIN      
        1 ccggtacgtt caacgtggcg atgttgccgc cgccaataat tccgatgcgc actcggtcca
       61 tgcggcctag cttcgcacgc gtatggacta cacgctcacc gaccgcacgc aggtgcgccg
      121 gctcccggcg cggggccggt acgaccgagc cctcgtgcac gaaatcgccg acgaagcgct
      181 cacatgcagc gtcggcttcg ccatcgacgg caagccgtgg gtgatcccca cgattcacgc
      241 gcgcgtcgac gacacgctgt acctgcacgg cgcgccggcg aaccacatgc tgcgatcgct
      301 cgcgagcggg gtggagacct gcgtgacgat cacgctcgtc gacgggctcg tgctcgcccg
      361 ctccgcgttc catcactcga tgaactaccg gtcggtcgtt gcgttcggtg tggcgaccaa
      421 ggtcgaagcg ttcgacgaaa agcgcactgc actcgccgcg ctcgtcgagc acgtcgttcg
      481 cggacgcggc atcgatgcgc ggccgccgac cgacgaggaa ctgcgcgcga cgctcgtcct
      541 gcgccttccg ctcgaggaag tgtccgccaa ggtgcgcacc ggcggcccga tcgacgacga
      601 agacgacttg ggcttgccgg tctgggcagg tgtgctgccg tgcaccacga cgtacggcac
      661 accggaaccc gacgccggcg tcgacgcggc ggtgccgggc tacgtcacca actacacgcg
      721 ctaggcgacc aatcccacga ctggctcctt gcggacctcg tgattcatga cgtccttcag
      781 gaggtccgtg agttccgatc gcactgatgc atacgcggga tcgtcgtagc ggttcacgat
      841 ctcaccggga tcttcttgca agtcgtagag ctcgccccac ggcgcctcga ggtagtggtg
      901 cagcttgtag cgcgtcgtcg tgatcgtgcg catgggcaac gacaccatga tgttgaagtc
      961 gttctcggtg agcacgtgtt cgcgcggcgt tccgtcgagt gcgcgcccct ccatccacga
     1021 cggtacgtcg acgccggcgg cgcgcagcat ggtcggcgca aggtcgatcg tgccgaccgg
     1081 gtcgtccaca acttctccgg cgccgacgcc gggaccgcgc aggatcagcg ggacgcgcag
     1141 gagcgagtcg tagccgaacg gacccttgaa gatcatctgg tgctcgccga ggaactcacc
     1201 gtggtcgctc gtgacgatca cgagtgtgtt gtccgccaat ccgcgcgccg cgagcgtgtc
     1261 gaggacgcgg ccgatcgcgt ggtcgagctg ggcaaccatg ccgtagtagc ccgccgtgag
     1321 cttcgccatc tgttcgcgcg tgtagttctt gccgcccggg ttggcccagt gcatgcccgg
     1381 ctggcgcaca ccctccgcga gcgcttcgtg tagccgcggc ttggttgtga actcgccatc
     1441 gcgtacgggg aacagaatct ccagcacatc ttccggttga tatcggtcgc accacggtgc
     1501 gggcgcgtcc atcggatggt gcggatccgt gaagctgatc cagccgaaga acggaccgtc
     1561 gacgtcgcgc aaccacgcac aggctcgatc agccgtccag gtcgtgcagt ggtcttcctc
     1621 cggtaacgcg ttgcgccacg tctgcgtgtc gtcccactgc tcccccaccg cctcgggctg
     1681 catgagccgt aggcgttcgg caccgcgctc gaagccgtcg cgaaagaggt actggccgta
     1741 gtgcaacccg aacggcggcg ggccgtagat ccaattccac tgacccatca ggtcggcgat
     1801 ccgcaggttg tgcccgaaca acatgagttc ggcgtgctcg aacccgaaat acgggccctg
     1861 ccactgcggg tcgacgcgcg ccgaaccctc gaccgattcg atcttgcccg tcggttgaaa
     1921 gggatacgcg gttgcgaagt gggccttgcc gatcaacgcg gttgcgtatc cggcgttcgc
     1981 aagcagcgtc gcgatcgagc ggccttccgc gtcgccgggg aggtcgacac cgttgcaggt
     2041 gacgccgtgc gtcgacggat aggtgccggt gaggatggtg gcgcgcgcgg gctggcagag
     2101 cgggttctgg gtccgcgcgc cgtcgtagcg cgtcccctcg gcggcgagcg cgtcgagtcg
     2161 cggggtcgca ccgagggggt tgccggtcga gccgagcgtg tcggcccggt gctggtcggt
     2221 cgtgatcagc aagacgttct gcgggggcat ttgcgtatcc tcctgcacat ggagacccta
     2281 cgcacacccg acgaacgatt cgcgagcctg cccggctatc cgttcgcccc gcactacgtc
     2341 accgtttccg acgacgaggg cggggagctc cgggtgcact acgtcgacga gggcccggcc
     2401 gacggcgcgg tggtgctgct catgcacggc gagccgtcgt gggcctacct gtaccgcaag
     2461 atgattccgg tgctgaccgc cgcgggctac cgctgcgtcg cgcccgacct cgtggggttc
     2521 ggtcgctccg acaagccgac caagcgcagc gactacacgt acgcgcgtca tgtcgagtgg
     2581 atgcgctcga cgctgttcga ccgcctggac ctgcgggacg tcacgctcgt gtgccaggac
     2641 tggggcggac tgctcggcct ccgcttggtc ggcgagcacc ccgaccgttt cgcgcgcgtc
     2701 gtcaccgcga acacgttcct tccgaccgga gacacgccgc ccggtgacgc attcttcaac
     2761 tggcagaagt tctcccagac cgtcgaagac ttcgacatcg ggttcatcat caacatgggt
     2821 tcggcgaccg atctctccgc cgaggtgatc gcggcgtaca acgcgccgtt ccccgacgac
     2881 tcgtacaaag agggtgcgcg ccagttcccg atgctcgtgc cgagccggcc cgacgatccg
     2941 gcatcgcccg ccaatcgcgc ggcatgggaa acgctgcgaa ccttcgacaa gccgtggctc
     3001 t
//
LOCUS       DASUGA010000186         5622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_730736, whole genome shotgun sequence.
ACCESSION   DASUGA010000186 DASUGA010000000
VERSION     DASUGA010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5622
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730736"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VFX21_03615"
     CDS             complement(<1..204)
                     /locus_tag="VFX21_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HEX5095069.1"
                     /translation="MSASRDIAVLGVGMHEWGKWGRNFVEYGMVAAQRALEDAGVVWN
                     DIQFVSGGETVRNGYPGYVAGATF"
     gene            complement(201..644)
                     /locus_tag="VFX21_03620"
     CDS             complement(201..644)
                     /locus_tag="VFX21_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019050543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX5095070.1"
                     /translation="MAKTRVPAAEGWFTMDEAAPQLVGARGSQSGSYYWPTAIATSGN
                     PTAPGEAREEALLSRRGHLWSWTTNHYAPPEPYVAPDPFVPYTVCAVELEAEQMVVLG
                     GLATGADPSVLEVGMEMELVLGPLYEDDDHEYVVWQWAPLSGGSK"
     gene            complement(644..1696)
                     /locus_tag="VFX21_03625"
     CDS             complement(644..1696)
                     /locus_tag="VFX21_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX5095071.1"
                     /translation="MTIRTPLCDLLDIEVPIVQTGMGWVAGARLVTATANAGALGILA
                     SATMTYDQLAHTIREVKARTDRPFGVNLRADAEDADARVQLLIQEGVKVASFALAPSE
                     TQIKTLKDAGVRVVPSIGAKRHAEKVAAWGVDAVVVQGGEGGGHTGAVATTLLLPQVC
                     DAVDVPVIAGGGFFDGRGLVAALAYGAAGVAMGTRFLLTQESTVPDAVKQYYLSRAVT
                     DTVVTARIDGVPHRVLRTEFVDDLEKSSPVTSYPRALKNALAFKKMSGISWKGMYQSG
                     RAMKHEGNLKWSQVVLAANTPMLLRAGLVEGNLDAGVLSSGQAAGVIDDLPTVAELVA
                     RIMSEAETTLKRLKEL"
     gene            complement(1701..2474)
                     /locus_tag="VFX21_03630"
     CDS             complement(1701..2474)
                     /locus_tag="VFX21_03630"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017236389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX5095072.1"
                     /translation="MIRDGAPTRAEVCAAAIADLFANDGEILANPIGTLPMIGGRLAK
                     ATVAPELVMTDGEALLVENIMPVGVDNPEKVVAGYNPYRAMFDVVWSGRRHVIMGGSQ
                     LDQYGNQNFAFIGDPAAPKTQLLGMRGAPGNTINDKTSYFVPSHSTRVFVPEVSVVSG
                     VGYDRAHALGGGGRFHNVVRVVTNLCVLDFETPDHRMRLRSVHPGVSVDDVVAATGFE
                     LVVDGDVPQSRLPTDDEVHWIRAVIDPNGLGEQEVPTPA"
     gene            complement(2471..3322)
                     /locus_tag="VFX21_03635"
     CDS             complement(2471..3322)
                     /locus_tag="VFX21_03635"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018215777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX5095073.1"
                     /translation="MPGPDKRVSIDEIVSEVHDGMTVGIGGWGSRRKPMALVRALCRS
                     SVRDLTVVSYGGPDVGLLCRAGKVRKVVYAFVSLDSIALEPHFRNARQSGAVSSMELD
                     EGMFLLGLQAAAWRVPFLPTRVGLGSDVMRLNPDLKTVTSPYADGDELVAMPALTLDV
                     ALVHMHRGDKGGAGQYLNVDPYFDDLMCMAATRRFMSVERIVETEDFLKEGPVQSVRI
                     NRLMTDGVVETPHGGHFTECVPDYGRDEAFQKEYVASAKNDDAWDAFRTKYVDVTEAE
                     YQQAVGQ"
     gene            3383..4684
                     /gene="aspS"
                     /locus_tag="VFX21_03640"
     CDS             3383..4684
                     /gene="aspS"
                     /locus_tag="VFX21_03640"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013020101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HEX5095074.1"
                     /translation="MRIWSDELGEHVGERVVLKGWLHNHRPLKKVDFVILRDAHGVCQ
                     IVANDEAARAEVAQWVDETVFEVEGTPKANPQAPGGVELVDPEFRVISASTDLPPINL
                     RVPELTEQLPFQLDHAAVSLRHPKRRAIFALGAASMGGMRRALDAEGFVEIQTPKMVA
                     SSTETGANVFRIDYFGERGYLAQSPQFYKQMMVGVFERVYEIGPVFRAEPHDTVRHLA
                     EYVSLDVELGFIDDHFTVMGIVRLVIDGMVAEMQARCARELELLELTLPKVPAEIPHV
                     FFRDAQEIVERETGEKGVVGEPDLAPAHERALGDWAKREHDSDFLFVTGYPMVKRPFY
                     THPDPARPEFSNSFDLLFRGLELITGGQRLHRYQDYLDALARQGQDPALLEGYLEAFR
                     YGMPPHGGFAIGLERWLARLVEAPNVRETTLFPRDLNRITP"
     gene            4687..5445
                     /locus_tag="VFX21_03645"
     CDS             4687..5445
                     /locus_tag="VFX21_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007228667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5095075.1"
                     /translation="MSFDGQVVLVTGGGRGIGRGIASAFAAEGASVVVCGRTEPESLP
                     NGVTFVACDVRDAEHVSALFATIAQRFGRLDVLVNNAGGAPPSDSATVSPRFTAGIIA
                     LNLTAAIVCAQGAYGLMAQQESGGSIVNIASVSGVRPSPNTAAYGAAKAGLLNLTETL
                     AVEFAPKVRVNAVTVGLVVTEQAHLFYGDDDGIAAVGRTVPLGRMADPSDIADVCLFL
                     ASPLARYVSGANVLVHGGGERPAYMDAARNTRPE"
BASE COUNT          845 a         1977 c         1899 g          901 t
ORIGIN      
        1 gaacgtcgcg cccgcgacgt accccgggta gccgttgcgc acggtctcac cgcccgagac
       61 gaactggatg tcgttccaca cgacgcccgc gtcctcgagc gcgcgctgcg cggcgaccat
      121 gccgtactcg acgaagttgc ggccccactt gccccactcg tgcatgccga caccgagcac
      181 ggcgatgtcg cgcgaggcgc tcatttcgac cctcccgaca gtggcgccca ctgccagacg
      241 acgtactcgt ggtcgtcgtc ttcgtagagc gggccgagca ccagttccat ctccatgccg
      301 acttcgagca ccgatgggtc ggcaccggtc gcgagcccgc cgagcacgac catctgctcg
      361 gcttccaact cgaccgcgca caccgtgtac ggcacgaacg gatcgggcgc gacgtagggc
      421 tcgggcggcg cgtagtggtt cgtcgtccac gaccacaagt gcccgcgccg cgacagcaac
      481 gcctcctcgc gcgcctcgcc gggcgcggtc ggattgcccg acgtcgcgat cgcggtcggc
      541 cagtagtaac tcccggactg cgagccgcgt gcgccgacga gctgcggcgc ggcttcgtcc
      601 atcgtgaacc agccttcggc ggccggaact cgtgtctttg ccatcacagt tccttcagtc
      661 tcttcagcgt cgtctcggct tcggacatga tgcgcgcgac gagctcggcg acggtcggca
      721 ggtcgtcgat gacgcccgcg gcctggccgc tcgacaacac accggcatcg aggttgccct
      781 cgacgagacc ggcgcgcagc agcatcggcg tgttggcggc gagcacgacc tgcgaccact
      841 tgaggttgcc ttcgtgcttc atcgcgcggc cggactggta catgcctttc cacgagatgc
      901 ccgacatctt cttgaacgcg agcgcgttct tgagcgcgcg cggatacgac gtgaccggcg
      961 acgacttctc caggtcgtcg acgaactccg tgcgcagcac acggtggggc acgccgtcga
     1021 tgcgcgccgt gacgaccgtg tcggtcaccg cgcggctcag gtagtactgc ttcaccgcgt
     1081 cgggaacggt gctctcctgc gtgagcagga accgcgtgcc catcgcgacg cccgcggcgc
     1141 cgtaggcgag cgcggcgacg aggccccgtc cgtcgaagaa cccgccgccc gcgatgaccg
     1201 gcacgtcgac cgcgtcgcag acctgcggga gcagcagcgt cgtcgcgacc gcgccggtgt
     1261 ggccgccgcc ctcgccaccc tgcacgacca ccgcgtcgac gccccacgcc gcgaccttct
     1321 ccgcatgacg cttggcgccg atcgacggca cgacgcgcac gcccgcgtcc ttgagcgtct
     1381 tgatctgcgt ttcgctcggc gcgagcgcga acgatgcgac cttcacgccc tcctggatga
     1441 ggagttgcac gcgtgcatcg gcgtcctccg cgtcggcgcg caggttgacg ccgaacggcc
     1501 ggtcggtgcg cgcctttacc tcgcggatcg tgtgcgcgag ctggtcgtac gtcatcgtcg
     1561 ccgacgcgag gatcccgagc gcgcccgcgt tcgccgtcgc ggtcacgagc cgcgcgcccg
     1621 cgacccagcc catgccggtc tgcacgatcg gcacctcgat gtcgaggaga tcgcagagcg
     1681 gcgtgcggat cgtcatcgcg tcacgccggc gtcgggactt cttgctcgcc gaggccgttg
     1741 gggtcgatga cggcgcggat ccagtgcact tcgtcgtcgg tcggcaggcg cgactgcggg
     1801 acgtcaccgt cgacgacgag ctcgaagccc gtcgccgcga cgacgtcgtc gaccgacacg
     1861 ccgggatgca ccgaacgcag ccgcatgcgg tggtcgggcg tctcgaagtc cagcacgcag
     1921 agattcgtga cgacgcgcac cacgttgtgg aaccggccgc cgccgccgag tgcgtgcgcc
     1981 cggtcgtagc cgacgccgct gacgacgctc acttcgggca cgaacacgcg cgtcgagtgg
     2041 ctggggacga agtacgacgt cttgtcgttg atcgtgttgc cgggtgcgcc gcgcatgccg
     2101 agcagttgcg tcttcggtgc cgcgggatcg ccgatgaatg cgaagttctg gttgccgtac
     2161 tggtcgagct ggctgccgcc catgatcacg tgccggcggc ccgaccacac gacgtcgaac
     2221 atcgcgcggt acgggttgta tcccgcgacg accttctcgg ggttgtcgac gccgaccggc
     2281 atgatgttct cgacgagcaa cgcctcaccg tcggtcatga cgagctccgg cgcgaccgtc
     2341 gccttggcga ggcggccgcc gatcatcgga agcgtgccga tcgggttggc gaggatctcg
     2401 ccgtcgttcg cgaacaggtc cgcgatcgca gccgcgcaca cctcggcgcg agtgggagcg
     2461 ccatcacgaa tcactggccg accgcctgct ggtattccgc ttcggtcacg tcgacgtact
     2521 tcgtgcggaa cgcgtcccac gcgtcgtcgt tcttggcgct cgccacgtat tccttctgga
     2581 acgcctcgtc gcggccgtag tcggggacgc actcggtgaa gtggccgccg tgcggcgtct
     2641 cgaccacgcc gtcggtcatg agccggttga tgcgcaccga ctgcaccgga ccttccttga
     2701 ggaagtcctc ggtctcgacg atgcgctcga ccgacatgaa gcgccgagtc gcagccatgc
     2761 acatgaggtc gtcgaaatag ggatcgacgt tgaggtactg gcccgcgccg cccttgtcgc
     2821 ctcgatgcat gtgcaccagc gcgacgtcga gcgtcaacgc cggcatcgca acgagctcgt
     2881 cgccgtcggc gtacggcgag gtcaccgtct tcaggtccgg gttcagccgc atcacgtccg
     2941 acccgagccc gactcgtgtc ggcaagaagg gcacccgcca cgccgccgcc tgcaggccga
     3001 ggaggaacat gccctcgtcg agctccatgc tcgacaccgc gccgctctgg cgcgcgttgc
     3061 ggaaatgcgg ttcgagcgcg atcgagtcga gcgagacgaa cgcgtacacg accttgcgga
     3121 ccttgcccgc gcgacacagg agcccgacgt cggggcctcc gtaggacacc accgtgagat
     3181 cgcgcaccga cgagcggcac agcgctcgca ccaacgccat cggcttgcgg cgtgaacccc
     3241 agccgccgat gccgacggtc atcccgtcgt gcacttcgct gacgatctcg tcgatggaaa
     3301 cgcgcttgtc cggaccgggc acgacgccgg atcgtatggc cgtttcaccg gtcgaggcca
     3361 acgcagcggt agcgtcttcc cgatgcggat ctggtcggac gagctcggtg aacacgtcgg
     3421 cgaacgagtc gtcctgaagg gctggttgca caaccaccgg cccctgaaga aggtcgactt
     3481 cgtgatcctg cgcgacgccc acggcgtctg ccagatcgtc gcgaacgacg aggccgcgcg
     3541 tgccgaagtc gcccagtggg tcgacgagac cgtcttcgag gtcgaaggca cacccaaggc
     3601 gaacccacag gcgccgggcg gcgtcgagct ggtcgacccc gagttccgcg tgatctcggc
     3661 gtccaccgac ctgccgccga tcaacctgcg cgtccccgag ctgaccgaac agctcccgtt
     3721 ccagctcgac cacgcggccg tctcgctccg ccatccgaag cggcgcgcga tcttcgcgct
     3781 cggcgccgcg tcgatgggcg gtatgcggcg cgcgctcgac gccgagggct tcgtcgagat
     3841 ccagacgccg aagatggtcg cgtcgtcgac cgagaccggc gccaacgtct tccgcatcga
     3901 ctacttcggc gagcgcggct acctggcgca gagcccgcag ttctacaagc agatgatggt
     3961 cggcgtgttc gagcgtgtgt acgagatcgg tcccgtgttc cgggccgagc cgcacgacac
     4021 cgtccgtcat cttgccgaat acgtatcgct cgacgtcgag ctcggtttca tcgacgacca
     4081 cttcacggtg atgggcatcg tgcgcctcgt gatcgacggc atggttgccg agatgcaggc
     4141 gcgctgcgcc cgtgagttgg agctgctcga gctcacgttg ccgaaggtgc cggccgagat
     4201 cccgcacgtg ttcttccgcg acgcgcagga gatcgtcgaa cgggagacgg gcgagaaggg
     4261 tgtggtgggc gaacccgacc tcgcgcccgc gcacgagcgc gcgctcggcg actgggccaa
     4321 gcgcgagcac gactccgact tcctcttcgt caccggctac ccgatggtga agcggccgtt
     4381 ctacacgcac cccgatccgg cgcggcccga gttctcgaac agcttcgacc tgttgttccg
     4441 tggcctcgag ctcatcaccg gcggccagcg tctgcaccgg taccaggact acctcgacgc
     4501 gctcgcgcgc cagggccaag atcccgcgtt gctcgagggc tatctcgaag cgttccggta
     4561 cggcatgccg ccgcacggcg gcttcgcgat cgggctcgag cggtggctgg cgcggctcgt
     4621 cgaggcgccg aacgtgcgcg agacgacact cttcccgcgc gacctcaacc gcatcacgcc
     4681 gtgaacatca gcttcgacgg gcaggtggtg ctcgtcaccg ggggcgggcg cggcatcgga
     4741 cgcggcatcg cgtcggcgtt cgcggccgag ggtgcgtcgg tcgtcgtctg cggccgcacc
     4801 gaaccggagt cgctgcccaa cggtgtgacc ttcgtcgcgt gcgacgtgcg cgacgccgag
     4861 cacgtgtcgg cgctgttcgc gacgatcgcg cagcggttcg ggcgcctcga cgtgctggtc
     4921 aacaacgcgg gcggcgcgcc gccatcggat tccgcgacag tgtcgccgcg tttcaccgcg
     4981 gggatcatcg cgttgaacct caccgcggcg atcgtgtgtg cacagggcgc gtacggcttg
     5041 atggcgcagc aggagagtgg cggttcgatc gtcaacatcg cgagcgtgag cggtgtgcgt
     5101 ccgtcgccca acacggctgc gtacggcgcg gccaaagccg gactgctcaa cctgaccgag
     5161 acgctcgcgg tcgagttcgc gccgaaagtg cgcgtgaacg cggtgaccgt cggcctcgtc
     5221 gtgacggagc aggcgcacct cttctacggc gacgacgacg gcatcgccgc ggtgggtcgc
     5281 acggtgccgc tcggccgcat ggccgatccg agcgacatcg cggacgtctg cctgttcctc
     5341 gcgtcaccgc tggcccgcta cgtcagcggc gccaacgtgc tcgtccacgg cggcggcgaa
     5401 agacccgcat acatggacgc ggcgaggaac acccgccccg agtagtcgcg taccggcgtg
     5461 gatctgacgc ggtgtgcgcg ccgaactcac gccggttcgt gtggcgcggc gtaccggcgt
     5521 ggatctggcg cggtgtgcgc gccgaactca cgccggttcg tgtggcgcgg cgtaccggcg
     5581 tggatctggc gcggtgtgcg cgccgaactc acgccggttc gt
//
LOCUS       DASUGA010000187         6510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_598877, whole genome shotgun sequence.
ACCESSION   DASUGA010000187 DASUGA010000000
VERSION     DASUGA010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6510
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598877"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VFX21_03650"
     CDS             complement(<1..354)
                     /locus_tag="VFX21_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX5095076.1"
                     /translation="MTGREVALAFAAVIGVVVIAVLGRRLGEMRLIAELERDEAARRR
                     QLAERLETEVRDARIAAAVSQQRAGFLSAISHNLRTPLTSIKAAASTLLSSWSRLEVE
                     ERRELVELIYDESDRL"
     gene            373..792
                     /locus_tag="VFX21_03655"
     CDS             373..792
                     /locus_tag="VFX21_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEX5095077.1"
                     /translation="MRVMLGADERTALTDAVRADLEGRGAEVVVVGPPAGDDQQWAEV
                     GQRVGAAVADGSVDTGVLFCWTGTGASIAANKVRGVRAALCTDAATAAGARRWNDANV
                     LVMSLRLTSPQVASEMLDAWFATEADPSEAGNIARLE"
     gene            802..1089
                     /locus_tag="VFX21_03660"
     CDS             802..1089
                     /locus_tag="VFX21_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019550282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX5095078.1"
                     /translation="MPTYVMLTTLGPDGWETVSKNPDRIRAVRDEVEALGLQVKAQYA
                     LMGQWDFMNIIEAPDEKAMAKAAVMLAARGTLRTTTLQAIEIEDLIATLKD"
     gene            complement(1090..1293)
                     /locus_tag="VFX21_03665"
     CDS             complement(1090..1293)
                     /locus_tag="VFX21_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095079.1"
                     /translation="MKPGSHVEVRSRFEDKWSRGFEVSEIVNNNGRDRYRVRRRSDGS
                     ILPVLFDETDLREEHRRGTWWMG"
     gene            1330..1995
                     /locus_tag="VFX21_03670"
     CDS             1330..1995
                     /locus_tag="VFX21_03670"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX5095080.1"
                     /translation="MVGTYPTLRFERKCWAAGDEIVVGIDEVGRGSWAGPVTVAAVVP
                     PPEHLSGVRDSKMLTPAEREVSARRVRAWARAIGVGHASHEECDVLGMTEALRRAALR
                     ALDQVADQGFRPDRIVLDGKHDYLGMPGRVHTIIKGDQKVLSVAAASVVAKVTRDAMM
                     ADEAEHFPAYGFESNRGYPAPVHKCALAAYGPSAIHRRSWIFMDYCLWGGVAKYERQP
                     RLF"
     gene            2005..3456
                     /gene="pgi"
                     /locus_tag="VFX21_03675"
     CDS             2005..3456
                     /gene="pgi"
                     /locus_tag="VFX21_03675"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX5095081.1"
                     /translation="MALEAAGWYFEPAHHQLSEQALAALVQRAEAAGLRSAIDAMFAG
                     DHINVTEDRSVLHVALRMPASASLVVDGVDVVREVHTVLEKMRAFSRRAARFRNVVNI
                     GIGGSDLGPAMAYDALWAYSDPARRFRFVSNIDGADLAFALHDLDAADTLFIVSSKTF
                     TTLETLTNARSARAWLVDALGEAAVAEHFVAVSTNAEAVTEFGIDTENMFEFWDWVGG
                     RYSMWSAIGLSLMCAIGADRFDEFLAGAHAMDEHFRTAPFAENIPVRMGLLTAYYREE
                     RDAQTHVVVPYAEVLGKLPSYLQQLEMESNGKSVRRDGSPVTTPTGEVVWGTAGTNGQ
                     HAYFQLLHQGTLVAPIDFIGFSRPQPGLDVAGHQDLLVSNMLAQSEALAFGTDDPALP
                     PYRVMPGNRPSSVFLADQLTPFALGALVAAYEHKVMTLGVLWDIDSFDQWGVELGKEL
                     ARKVAAEMQSGAEPALHHDDATNALIRRYRSRR"
     gene            complement(3453..4760)
                     /locus_tag="VFX21_03680"
     CDS             complement(3453..4760)
                     /locus_tag="VFX21_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095082.1"
                     /translation="MTVTEPRAVPTTRDRAAVLALGAWFVLIVVASLWGEHFGAGRIG
                     LGVPPLHSSWRPRPGLDTLLAVGTGAGALLIAPRIVDRLRWRFVPPFAGALAGLWAVV
                     LAANDGTKGLTRGAVTSDYREVARGIDAPLVFLDRFSASVRAHELPVHTSGHPPGFVV
                     VLWVLDHLGLRADVWPTALCIGGGTLAIACIAITVREVAGEPVGRAAVPFLALSPAAI
                     WIATTPDAFFAGVGAAAVCATVLATSNRRTMAPAIAGGIAFAAAALLSYGLVLLAVIP
                     VVVAVARRTVRPLVVAGSTAAAALCVVAFTTGFWWYDGLQATRDQYWLGIASARPFRY
                     FVVANLAALAITCGPAAAVGLAWLRDRALWLLVGGAVAAVLVADLSAMSKGEVERIWL
                     PFALWILPAAATATCARAPRTARALLALQVGTALVVQTFLHSA"
     gene            complement(4757..5803)
                     /locus_tag="VFX21_03685"
     CDS             complement(4757..5803)
                     /locus_tag="VFX21_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5095083.1"
                     /translation="MRVLVTGGAGFVGSHVVDQLLGAGHDVRVVDALDPSLHREPPVF
                     VDPRVELVPGDIRNPNVAACVVDGVDAVSHQAAMVGLGVDFDDVTDYVDHNALATAVL
                     LRALHRRDFRGRFVLASSMVVYGEGRYRCAVHGLIRPLPRTSLQLQRGDFEPACPGCG
                     AALAPESVPESAPIDPRNVYAATKLHQEHLCDAFARTHPDAAVTVLRYHNVYGPRMPR
                     DTPYAGVASIFRSAIERGEAPRVFEDGGQLRDFVHVRDVARANVLALTVEEPVRDVCN
                     VASGTPRTVLDMANALAAAAPTRPTPVVVGSGRLGDVRHVFASTARAYELLGFRAETD
                     FAAGMREFATAPLR"
     gene            complement(5800..>6510)
                     /locus_tag="VFX21_03690"
     CDS             complement(5800..>6510)
                     /locus_tag="VFX21_03690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5095084.1"
                     /translation="EARARERALESSRRELVAWISHDLRTPLSGLRAIAEALEDGVVG
                     DDAPRYYALLREETERLSVLVDDLFELSRTQAGVLRLELQRVQLGEVVSDAIAGMAPV
                     AAAKGVRLESRDLDGLPDCSVAPPELLRALRNLLENAVRHTPADGTVLVETTLDGPDA
                     VVSVIDDGGGIADADLPRVFDTGFRGDQARGGDAGAGLGLAIAKSIVEAHHGELAVCN
                     DNGGARFTIRLPLGTHPR"
BASE COUNT         1022 a         2209 c         2352 g          927 t
ORIGIN      
        1 gaggcggtcg ctctcgtcgt agatcagctc gacgagctcg cggcgctctt cgacttcgag
       61 gcgcgaccac gacgacagca atgtcgacgc cgcggccttg atcgacgtca acggcgtccg
      121 caggttgtgg ctgatcgccg agaggaagcc ggcgcgctgt tggctgaccg ccgccgcgat
      181 gcgcgcgtcg cgcacttcgg tctcgagccg ttccgcgagt tgccggcggc gcgcggcttc
      241 gtcgcgttcc agctcggcga tcaggcgcat ctcgccgagc cgccgcccca acaccgcgat
      301 gacgacgacg ccgatcacgg ccgcgaacgc gagggccacc tcccttccgg tcacggtcat
      361 atcctgcacc gtgtgcgagt gatgcttggt gccgacgagc gcacggcgct cacggacgcg
      421 gtccgggccg acctggaagg gcggggcgcc gaggtcgtcg tggtcggtcc gccggcgggc
      481 gacgatcagc agtgggccga ggtgggacag cgggtcggcg ccgcggtggc cgacggatcg
      541 gtcgacaccg gtgtgctgtt ctgctggacc ggcaccggcg cgtcgatcgc ggccaacaag
      601 gttcggggcg tgcgcgcggc gttgtgcacc gacgccgcga ccgcagccgg cgcccgccgc
      661 tggaatgacg cgaatgtcct cgtgatgagc ttgcggctga cgagtccgca ggtcgcgtcg
      721 gagatgctcg acgcgtggtt cgcgacggag gcggacccgt cagaagccgg caacatcgcg
      781 cggctggagt agcgttcact cgtgcctacg tacgtcatgc tcaccacgct cggtcccgac
      841 ggttgggaga cggtgagcaa gaaccccgat cgcatccgcg ccgtgcgcga cgaagtcgaa
      901 gcgctcgggc tccaggtcaa ggcgcagtac gcgctgatgg gtcagtggga tttcatgaac
      961 atcatcgaag cgcccgacga gaaggcgatg gcgaaggccg cggtcatgct cgcggcgcgc
     1021 ggcacactgc gcacgacgac gttgcaagcg atcgagatcg aagacctcat cgcaacgttg
     1081 aaggattagc taccccatcc accaagtgcc gcggcggtgt tcctcgcgca ggtcggtctc
     1141 gtcgaagagc accggcagga tcgaaccgtc ggagcggcgg cgcactcggt agcggtcgcg
     1201 gccgttgttg ttgacgatct ctgatacctc gaagccacga ctccacttgt cttcgaagcg
     1261 agagcgcacc tcgacgtgac tgccgggctt cacgaggtgg agcataagca cctgctagct
     1321 tccgcgcgcg tggtcggcac ttacccgacg ttgcgattcg agcggaagtg ttgggcggcc
     1381 ggcgacgaga tcgtggtcgg gatcgacgag gtcggccgtg gatcttgggc gggcccggtc
     1441 accgtagcgg ccgtggtgcc ccctccggaa cacctcagcg gtgttcgcga ctcgaagatg
     1501 ttgacgcccg cggagcgcga ggtgagcgcg cgtcgcgtgc gcgcctgggc gcgcgcgatc
     1561 ggtgtcggcc acgcgtcgca cgaggagtgc gacgtgctcg gcatgaccga agcgctgcgc
     1621 cgcgccgcgc tgcgggcgct cgaccaggtc gctgaccagg gctttcggcc cgaccgcatc
     1681 gtgctcgacg gcaagcacga ctatctgggt atgcccggcc gcgtgcacac gatcatcaag
     1741 ggcgaccaga aagtgctctc cgtggcggcc gcgtcggtgg tcgcgaaggt gacgcgcgac
     1801 gcgatgatgg cggacgaggc cgagcacttc ccggcgtacg gcttcgagtc gaaccgcggt
     1861 tacccggcgc cggtgcacaa gtgcgcgctg gccgcgtacg gcccgtccgc gatccaccgc
     1921 cggtcctgga tcttcatgga ctactgcctg tggggcggcg tcgcgaagta cgaacgtcaa
     1981 ccacgattgt tctgaggttc tgctgtggcc ctcgaagcgg caggctggta cttcgaaccc
     2041 gcacaccatc agctctcgga gcaggcgctc gcagcgttgg tgcagcgtgc ggaggcggcc
     2101 gggttgcggt cggcgatcga tgcgatgttc gcgggcgacc acatcaacgt caccgaggac
     2161 cggtcggtgc tgcacgtcgc actgcggatg ccggcttcgg cgtcattggt ggtcgacggt
     2221 gtcgacgtcg tacgcgaggt ccacacggtc ttggaaaaga tgcgcgcgtt cagccggcgc
     2281 gccgcgcggt tccgcaatgt cgtcaacatc ggcatcggcg gcagcgacct cgggccggcc
     2341 atggcgtacg acgcgctgtg ggcatactcc gatccggccc ggcggttccg gttcgtgtcg
     2401 aacatcgacg gcgccgatct cgcgttcgcg ctccacgacc ttgatgcagc cgacaccttg
     2461 ttcatcgtgt cgtcgaagac gttcacgaca ctcgagacct tgaccaacgc ccgctcggcg
     2521 cgggcctggc tggtcgacgc gctcggcgag gccgcggtgg ccgagcactt cgtcgcggtg
     2581 tcgaccaacg ccgaagcggt cacggagttc gggatcgaca ccgagaacat gttcgagttc
     2641 tgggactggg tcggcggccg ctactcgatg tggtccgcga tcgggctgtc gctcatgtgc
     2701 gcgatcggcg ccgaccgctt cgacgagttc ctcgcgggcg cgcacgcgat ggacgagcac
     2761 ttccgcaccg cgccattcgc cgagaacatc cccgtgcgca tgggtctgct gaccgcgtac
     2821 taccgcgagg agcgcgacgc gcagacgcac gtggtggtgc cgtacgcgga agtgctcggg
     2881 aagctgccgt cgtacctaca acagctcgag atggagagca acggcaagtc ggtgcggcgc
     2941 gacggctcgc cggtgacgac gccgacgggt gaagtggtgt gggggacggc cggcacgaat
     3001 ggccagcacg cctacttcca gctgctgcac cagggcacgc tggtcgcgcc gatcgacttc
     3061 atcgggttct cccggccgca accgggtctc gacgtggccg ggcatcaaga cctgctcgtg
     3121 tcgaacatgc tggcgcagtc ggaagcgctc gcgttcggca ccgacgatcc ggcgctgccg
     3181 ccgtatcgcg tgatgcccgg caaccgcccg tcgagtgtgt tcctggccga tcaactcacg
     3241 ccgttcgcgc tgggcgcgct cgtcgccgcg tacgagcaca aggtgatgac gctgggtgtg
     3301 ctgtgggata tcgactcctt cgaccaatgg ggcgtcgagc tcggcaaaga gctggcgcgc
     3361 aaagtcgcag ccgagatgca gtcgggtgcc gagcccgcgc tgcatcacga cgacgcgacc
     3421 aacgcgctca tccgccgcta ccgctcgcgc cgttaggcgc tgtgcaggaa tgtctgcacg
     3481 acgagcgcgg tgcccacttg cagtgccagg agtgcacggg ccgtgcgcgg cgcacgcgcg
     3541 cacgtggccg tggccgccgc ggggaggatc catagtgcga acggcagcca gatccgttcg
     3601 acttcgccct tcgacatcgc gctgagatcc gcgaccaaca ccgccgcaac cgcgccgccg
     3661 accaagagcc acaacgcccg atcgcggagc cacgcgagtc cgacggccgc cgcgggcccg
     3721 catgtgatgg cgagggcggc gaggttcgcg acgacgaagt agcggaacgg ccgtgcggac
     3781 gcgatgccga gccagtactg atcacgcgtc gcctgcaagc cgtcgtacca ccagaacccg
     3841 gtcgtgaacg ccacgacgca gagcgcggcg gccgcggtcg atcccgcaac gaccagtggc
     3901 cggaccgtcc gccgcgcgac ggcaacgacg accgggatca cggccaggag cacgaggccg
     3961 tacgagagca gggcggctgc cgcgaacgcg atgccgcccg cgatcgccgg cgccatcgtc
     4021 cgccggttcg aggtcgcgag caccgtcgcg cagacggccg ccgcacccac gccggcgaag
     4081 aacgcgtcgg gtgtcgtcgc gatccagatc gcggctggtg acagcgcgag aaaggggacg
     4141 gccgcgcgcc cgacgggttc cccggcgacc tcgcgcacgg tgatcgcgat gcatgcgatc
     4201 gcgagtgtgc cgccgccgat gcacagtgcc gtcggccaga cgtcggcgcg cagtccgagg
     4261 tggtcgagca cccacaacac gacgacgaac ccgggcggat gtcccgacgt gtgcacgggc
     4321 aactcgtggg cgcgcacgga cgcgctgaat cggtcgagga agacgagggg cgcatcgatg
     4381 ccgcgcgcga cctcgcgata gtcgctcgtc accgcgccgc gggtcaggcc cttggtgccg
     4441 tcgttggcgg cgagcacaac ggcccacagt cccgcgagcg cgcccgcgaa cggcggtacg
     4501 aagcgccacc gcaaccggtc gacgatccgc ggcgcgatca ggagtgcacc ggcgccggtg
     4561 ccgaccgcca ggagcgtgtc gagaccgggt cgcggccgcc acgacgaatg caggggcggc
     4621 acgccgagcc cgatgcgacc cgcgccgaag tgctcgcccc agagggaggc cacgacgatg
     4681 aggacgaacc acgcgcccaa tgcgagcacg gcggcgcggt cgcgtgtcgt cgggaccgcg
     4741 cgcggctcgg tgaccgtcac cgcaacggcg cggtcgcgaa ctctcgcatg cccgccgcaa
     4801 agtcggtttc ggcgcgaaag ccgagcaact cgtacgcgcg tgcggtcgac gcgaagacgt
     4861 ggcgaacgtc gccgaggcgt ccgctgccga cgacgaccgg cgtcgggcgc gtcggcgcgg
     4921 ccgcggcgag tgcgttcgcc atgtcgagga ccgtgcgggg ggtgccgctc gcgacgttgc
     4981 acacgtcgcg gacgggctcc tcgaccgtga gcgccaacac gttcgcgcgc gcgacgtcgc
     5041 gcacatggac gaagtcacgg agctgcccgc cgtcctcgaa cacgcgcggt gcctcgcctc
     5101 gttcgatcgc gctgcggaag atgctcgcga ctcccgcata tggcgtgtcg cggggcatcc
     5161 gcggcccgta cacgttgtgg taacgcagca cggtgaccgc ggcgtcggga tgcgtgcgtg
     5221 cgaacgcgtc acacaggtgt tcctgatgca gcttggtggc ggcgtacacg ttgcgcggat
     5281 cgatgggtgc agattcgggc acggactccg gcgcgagcgc ggcgccgcag ccgggacacg
     5341 ccggctcgaa gtcgccgcgt tgaagctgca acgaggtgcg cggcaagggc cggatcaacc
     5401 cgtgcaccgc acagcggtag cgaccttcgc cgtagacgac catgctgctc gcgagcacga
     5461 agcggccccg gaagtcgcgg cggtgcagcg cacgcagcaa cacggccgtc gccagcgcgt
     5521 tgtggtcgac gtagtcggtg acgtcgtcga agtcgacgcc gagcccgacc attgctgcct
     5581 ggtgcgacac cgcgtcgacg ccgtcgacga cgcacgccgc gacgttcggg ttgcggatgt
     5641 cgccgggcac gagctccacg cgcggatcga cgaacaccgg cggctcgcgg tggagcgacg
     5701 ggtcgagcgc atcgacgaca cgtacgtcgt gccccgcacc gagcagctgg tcgacgacat
     5761 gggatccgac gaacccggcg ccgccggtga ccaggactct caacgggggt gcgtccccag
     5821 cggcaggcgg atcgtgaagc gggcgccgcc gttgtcgttg cacaccgcga gctcgccgtg
     5881 gtgcgcctcg acgatgctct tggcgatggc gagcccgagc ccggcgccgg catcgccgcc
     5941 tcgggcctgg tcaccgcgga agccggtgtc gaacacgcgg ggcagatcgg cgtcggcgat
     6001 gccgccgccg tcgtcgatga ccgacacgac cgcgtcgggc ccgtcgagcg tggtctcgac
     6061 gagcaccgtg ccgtccgcgg gtgtgtggcg caccgcgttc tcgagaaggt tgcgcaacgc
     6121 ccgcagcagc tcgggcggcg cgaccgagca gtcgggcagg ccgtcgaggt ctcggctctc
     6181 gagtcgcaca cccttggccg cggcgaccgg tgccatgccg gcgatcgcgt cggagacgac
     6241 ctcgccgagc tgtacgcgct gcagttcgag tcgcaacacg cccgcttgcg tccggctcag
     6301 ctcgaacagg tcgtcgacga gcacgctcag gcgttcggtc tcctcgcgca acagcgcgta
     6361 gtagcgcggc gcgtcgtcac cgacgacgcc gtcctcgagt gcttcggcga tcgcgcgcag
     6421 acccgacagc ggcgtgcgca ggtcgtggga gatccacgcg acgagctcgc ggcgcgacga
     6481 ctcgagggcg cgctcgcggg cgcgagcctc
//
LOCUS       DASUGA010000188         4209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1980659, whole genome shotgun sequence.
ACCESSION   DASUGA010000188 DASUGA010000000
VERSION     DASUGA010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1980659"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..365)
                     /locus_tag="VFX21_03695"
     CDS             complement(48..365)
                     /locus_tag="VFX21_03695"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEX5095085.1"
                     /translation="MGNGNDFVPLGDALAAVGKELGLPDPNVVSRIETVWAAVVGPAL
                     ASHSEVRSLREGVCSVTVDSASWATQLRYLHDDLVHRLCEALGAGSVTAIRVAVSPPA
                     GAG"
     gene            complement(369..1493)
                     /locus_tag="VFX21_03700"
     CDS             complement(369..1493)
                     /locus_tag="VFX21_03700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEX5095086.1"
                     /translation="MESPASHVGRVWLTDFRCFDGLELALTPGVTVLHGANGQGKTSL
                     LEAIGWVARARSFRGVADALLVRSGAEQAVVRAEVVSGDRTQLFEAEIRVSGRNRIMT
                     NKQVLTRARDLHGLLRVTVFAPDDLALVKDGPAERRTYLDELLAMISARYDAARGDYE
                     KVLRQRNALLRGGMRDEHARSTLAVFDEQLVHAGAELLRGRLRLIERLVPSVDTAYRT
                     LAPDHRAVTAEYVTDWAGDALDATEEGALEAQLRDGIARKTKAEIDRGVTLVGPHRDE
                     WRLAIGGLEARTQASQGEQRTLALALRLAGHGAVTDIVGAAPVLLLDDVFSELDADRS
                     HALVHNLPAGQTLVTTAGHVPDDIEPQQVLRVDHAKITPA"
     gene            complement(1497..2591)
                     /gene="dnaN"
                     /locus_tag="VFX21_03705"
     CDS             complement(1497..2591)
                     /gene="dnaN"
                     /locus_tag="VFX21_03705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX5095087.1"
                     /translation="MKFRCERDTLAEAVATAQRTVASRSGALPVLQDLRISATGGGLE
                     LVGSDLEITNRVQVPADVEEEGVAVVPKLLGEIVRKLEPGPVTVAVTGDEAVITAGRF
                     STSLRLKPAEDYPRLAPSDGEGVTVDAAAFAAALKQVVRAASKDDLRPILTGVLLTAH
                     AGGLRLVATDSYRLAVRDLKGVSMLSEGQRVLVAAKGLSEVQRLAGDGEIEVVLRERD
                     VVFRTSRAEVTARLIEGEFPNYEQLIPSGYPNRLTVPREAFDDALERVQIVGQNRDNA
                     AVRLSMSSSGLELSMSAQDVGNAQESLDAKFEGTELTVAFNPGFLREGVEAIDTAEVA
                     LETIDPLKPATLRAADGGDFLYLLMPVRTS"
     gene            complement(2802..>4209)
                     /gene="dnaA"
                     /locus_tag="VFX21_03710"
     CDS             complement(2802..>4209)
                     /gene="dnaA"
                     /locus_tag="VFX21_03710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX5095088.1"
                     /translation="WAKVAAAVRAQLSEATWNTWFQGVHALDLTDESLLLGVPSSVAV
                     ERLRTSYLGLLADAVQALTGVPIAVELLVDTAPRHEELVSIDTLGTAPNPAGPSTAVD
                     DRPRPPAGDTEQAPAAAPSSSATSLNPRYTFDQFVIGASNRFAHAAALSVAESPARSY
                     NPLFIYGPAGLGKTHLLHAIGHHDRELFSSKRVRYVSTETMMNEFVEAMRSKTMPGFK
                     RRYREVDVLLVDDIQFLERTEQLQEEFFYTFNDLHSRGSQIVISSDRPPKSIATIEDR
                     LRSRFEWGLITDIQPPEFETRLAILRKKAESEHLDGIPDPVLGFIAENVVDNVRELEG
                     SLIRVAAYSSLHRVPLTEEVAREVLADLLPATRPRVITPQLILEETAKMFGWTVEDLC
                     GKSRRRPLVTARQIGMYVFRELTDYSYPKIAEEFGGRDHTTVMHACEKIRGQMAERRV
                     VFEQVNELINRIKHGASG"
BASE COUNT          656 a         1429 c         1438 g          686 t
ORIGIN      
        1 tcagaggggg tttcgcgtag gtgatgattc taccagaacg gagtgactta accggccccc
       61 gcaggggggc tcacggccac ccgaatcgcg gtcaccgaac cggcaccgag ggcctcgcac
      121 agccggtgca cgagatcgtc gtggaggtac cgcaactgcg ttgcccacga tgcgctgtcg
      181 acggtcaccg agcacacgcc ctcacgcagc gaacgcacct cggaatgact cgcaagcgcg
      241 ggcccgacga ccgcggccca cacggtctcg atgcgcgaga cgacattcgg atcgggcagg
      301 ccgagctctt tgccgaccgc cgcgagcgcg tcgccgagcg gcacgaagtc gttaccgttg
      361 cccatgactc atgccggtgt gatcttcgcg tggtcgacgc gcagcacttg ttgcggttcg
      421 atgtcgtcgg gcacatggcc ggcggtggtc accaacgtct ggcccgccgg caggttgtgc
      481 accagcgcgt gcgaacggtc ggcgtcgagc tcgctgaaga cgtcgtcgag caacaacacc
      541 ggcgccgcgc cgacgatgtc ggtgacggca ccgtggcccg cgagccgcaa ggccagcgcg
      601 agcgtgcgct gctccccttg cgacgcctgc gtgcgggctt cgagcccgcc gatcgcgagc
      661 cgccattcgt cgcggtgagg tccgacgagt gtgacgccgc gatcgatttc ggcctttgtt
      721 ttccgggcga ttccgtcgcg tagctgtgcc tcgagcgcgc cttcctcggt cgcgtcgagg
      781 gcgtcgccgg cccagtcggt cacgtactcg gcggtgaccg cacggtgatc gggcgccagc
      841 gtccggtacg ccgtgtcgac gctggggacg agccgctcga tcagccggag ccggccccgt
      901 aacagctcgg cgccggcgtg cacgagctgc tcgtcgaaca ccgcgagcgt cgaccgcgcg
      961 tgctcgtcgc gcataccgcc ccgcaacaac gcgttgcgtt gccggagcac tttctcgtag
     1021 tcgccgcgcg cggcgtcgta gcgggccgag atcatcgcca gcagctcgtc gagatacgtg
     1081 cgccgctcgg ccggcccgtc cttgaccagc gccaggtcgt cgggggcgaa caccgtgacc
     1141 cgcaacaagc cgtgcagatc acgagcacgc gtgagcacct gcttgttcgt catgatccgg
     1201 ttgcggccac tcacccggat ctcggcctcg aacaactggg tgcggtcgcc ggagacgacc
     1261 tcggcccgca cgactgcttg ctcggcgccg ctgcgtacca acaacgcgtc ggcgacaccg
     1321 cggaaggacc gcgcgcgcgc cacccaaccg atggcctcga ggaggctcgt cttgccctgc
     1381 ccgttcgccc cgtgcagcac cgtgacaccc ggcgtcagcg cgagctcgag gccgtcgaaa
     1441 caacggaagt ccgtgagcca gacccgtccg acgtgcgacg cgggcgactc catcggctac
     1501 gacgtgcgga cgggcatcag caagtagagg aagtcgccgc cgtcggccgc ccgcagcgtc
     1561 gcgggcttca gcgggtcgat cgtctccagc gcgacctcgg ccgtgtcgat ggcctcgacg
     1621 ccctcccgca agaaccccgg gttgaaagcg acggtcaact cggtgccttc gaacttcgcg
     1681 tccagcgact cctgcgcgtt gccgacgtcc tgggccgaca tcgatagctc cagccccgac
     1741 gagctcatcg acagccgtac cgcggcgttg tcgcggttct gcccgacgat ctgcacccgc
     1801 tcgagcgcgt cgtcgaacgc ctcccgcggc acggtcaggc ggttcgggta tcccgagggg
     1861 atgagctgtt cgtagttcgg gaactcgccc tcgatcagcc gggccgtcac ctccgcgcgc
     1921 gaggtgcgga acacgacgtc tcgctcgcgc aacacgactt cgatctcgcc gtcaccggcc
     1981 agccgctgca cttcgctgag ccccttggcc gccaccagga cccgctggcc ctccgagagc
     2041 atgctgacgc ccttgaggtc ccgcacggcc agccggtagg agtcggtggc caccaggcgc
     2101 aggccgccgg catgcgcggt gaggagcaca ccggtgagga tgggccgcag gtcgtccttg
     2161 gatgccgccc gcaccacctg cttgagcgcc gccgcgaacg cagccgcgtc gacggtgacg
     2221 ccctcgccgt cgctgggcgc cagacgtggg tagtcctcgg ccggcttcaa ccgcaacgac
     2281 gtcgagaacc ggccggcggt gatgactgcc tcgtcaccgg tgacggcgac cgtgaccgga
     2341 ccgggttcga gcttgcgcac gatctcgccc aacagcttcg ggacgacggc aacgccttct
     2401 tcttcgacgt cggccggcac ctgcacgcgg ttggtgatct ccaagtcgga gccgacgagc
     2461 tcgagcccac cgccggtggc ggagatgcgc aggtcctgga ggacgggaag cgcgccactc
     2521 cggctggcga ccgtgcgctg ggccgtcgcc accgcttcgg cgagggtgtc acgctcgcat
     2581 cggaacttca cctgggcctc ctagaacaca aaaggaattc tctcttgtag tagcagtagt
     2641 agggcctgtg gattgtggac aaccgaggct tacacctggt cgacgccagt gtgggcctgg
     2701 gcacggacat ccacagctgt ccccagcatg ccaccgcgtt acgtggctga gccgctgcac
     2761 atcccacgcg cggcgcggga tttccccaga gatccacagg cttatccact ggcaccgtgc
     2821 ttgatccggt tgatgagctc gttgacctgc tcgaacacga cccggcgctc ggccatctgg
     2881 ccgcggatct tctcgcacgc atgcatcacg gtcgtgtggt cgcggccgcc gaactcctcg
     2941 gcgatcttcg ggtagctgta gtcggtcagc tcgcggaaca cgtacatgcc gatctggcgc
     3001 gcggtgacga gcggccggcg gcgcgacttg ccgcagaggt cttcgaccgt ccatccgaac
     3061 atcttcgccg tttcctcgag gatcagttgc ggcgtgatga cgcgtggacg cgtcgccggc
     3121 aacaggtccg cgagaacttc gcgcgcgacc tcttcggtca agggaacacg atgcaaactc
     3181 gagtaggccg cgacgcgaat gagtgagcct tcgagctcgc ggacgttgtc gacgacgttc
     3241 tccgcgatga acccgagcac ggggtcgggg atgccgtcga ggtgctcgga ttcggccttt
     3301 ttcctgagga ttgcgagccg cgtctcgaac tcggggggct ggatgtcggt gatgaggccc
     3361 cactcgaaac gactgcggag gcggtcttcg atcgtcgcga tcgacttcgg gggacggtcg
     3421 gaggagatga cgatctggct gccgcggctg tgcaagtcgt tgaacgtgta gaagaactct
     3481 tcttgcagtt gctcggtgcg ttcgaggaac tggatgtcgt cgacgagcaa cacgtcgact
     3541 tcgcggtagc ggcgtttgaa gcccggcatc gtcttcgagc gcatcgcctc gacgaactcg
     3601 ttcatcatcg tctcggtcga cacgtagcga acgcgcttcg acgagaacag ctcgcggtcg
     3661 tggtgtccga tcgcgtgcag aagatgcgtt ttcccgaggc ccgcggggcc gtagatgaac
     3721 aacgggttgt acgagcgcgc cgggctctcc gcgaccgaca acgcggccgc gtgcgcgaac
     3781 cggttcgacg cgccgatgac gaactggtcg aacgtgtagc gcgggttcag tgacgttgcg
     3841 ctgctgctcg gcgcggccgc gggcgcctgt tcggtgtcac ccgccggggg gcgcgggcga
     3901 tcgtcgacgg cggtgctcgg cccggccggg ttcggcgcgg tgccgagggt gtcgatcgag
     3961 acgagctctt cgtggcgggg tgcggtgtcg acgaggagct cgaccgcgat ggggacaccg
     4021 gtcagggcct gcaccgcgtc ggcgagcaag ccgagatagc tggtgcgcag ccgctccacc
     4081 gcgaccgagc tcgggacgcc gagcaggagg gactcgtcgg tgaggtcgag cgcgtgcacc
     4141 ccttggaacc atgtgttcca agtcgcctcc gagagctggg cgcgcacggc ggccgccacc
     4201 ttggcccag
//
LOCUS       DASUGA010000189         5966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1285828, whole genome shotgun sequence.
ACCESSION   DASUGA010000189 DASUGA010000000
VERSION     DASUGA010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5966
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285828"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VFX21_03715"
     CDS             complement(<1..186)
                     /locus_tag="VFX21_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095089.1"
                     /translation="MTASGWYLLVSIWGACFTVMALWPIRRPQWLMGIGFFASWLATE
                     LAVIHIVWQVIATIVFIW"
     gene            285..1679
                     /locus_tag="VFX21_03720"
     CDS             285..1679
                     /locus_tag="VFX21_03720"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX5095090.1"
                     /translation="MQRLTGLDAAFLALETPSAHMHVMGVAIVDPTTAPRGFSYERVR
                     EVMASRLDLVGPLRRRVIEVPLGLHNPVWIEDPDFDLDFHLRRAALPAPGGEAELAAF
                     VADVAGRELTRDRPLWEAWVVEGLEHGYYAFVAKLHHSLIDGASGVEILAALFDMEPA
                     PEPRVADIVEEWHPDHIPSDFELLAYAVLSFAQRPLYFVKAANNLGRGLMSAVQRARE
                     ESLDVALPLTAPRLSMNRTITPHRKVAFASVPLADIKAAKNALGVTVNDIVLAIASGA
                     LRNYLQQREELPDRSLVAAIPTSIRAEGEREYGNRVSAMFAALPVELDDAIDRVHAVQ
                     RSTAGAKGVHEQIGSTTLQEWAEVAAPAVFSRAMRLYTRLRIGERVAPVINAVVSNVP
                     GPPFPLYLAGARLVALHPLGPIFDDCGLNLTVISYLDHVDFGFIACRELIPDLEELAA
                     AVPEALAEIVKAAG"
     gene            complement(1663..2475)
                     /locus_tag="VFX21_03725"
     CDS             complement(1663..2475)
                     /locus_tag="VFX21_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020640392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX5095091.1"
                     /translation="MAYTLVVFHAHPDDEAIATGGVMAQAHAQGHRVVLVVATRGELG
                     EYDPAILEPGETLADRRVAETQAAAKVLGVDRVEFLGYRDSGMAGEDSNHATGAFANA
                     DLEEAAQKLAALLVGEHADVLTVYDEFGNYGHPDHIQVHRVGVRAAEIAQTTVVLEAT
                     MNRDHLLRLMEMIPDGLDDVSEEERPTPEDIEHLGMPESALTTAVDVRDFVELKRAAM
                     AAHASQISDASFFLQMPIDAFRESFGYEWFIRRGAEPGLRESSLFETLSPRP"
     gene            2599..3948
                     /locus_tag="VFX21_03730"
     CDS             2599..3948
                     /locus_tag="VFX21_03730"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5095092.1"
                     /translation="MRGSKLVTLGLALTLLLAACGNSDDDDDSAAPTTGAGGSTTPTT
                     ASDLTKNVPIDEVGVTDDEIHVNVITAKTNPVGGKYAELADGINAYFAMINDGGGIYG
                     RQLKITKERDDQLANNQQETQAALAEDQAFATFEATLLFTGADLLDEADQPTFIWNIN
                     PEMAGHDNIFANASALCFGCDGHILPYMAEKHEATKIGILAYGVSEESKRCAAGNRSS
                     IEKYGAALGQEVAFFDDSIGFGQELSAQVAQMKSNGVQFITTCMDASETLVLAKEMKK
                     QGLDAVQHLPNGYDHQLMTDNAAEFEGSIVIPQFLALETAEPLQIQKDFEKWTKEIGV
                     TPVETTIVGWILAHEFVEGLKAAGPEFNKAKLIAALNAMTDYDAEGMIPPIDWTKGHI
                     DPSTNPEVRGQLECANFTVVQGGNFVPEWVDDPAKPWTCFNETDPDITKPIPTSFAE"
     gene            3972..>5966
                     /locus_tag="VFX21_03735"
     CDS             3972..>5966
                     /locus_tag="VFX21_03735"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5095093.1"
                     /translation="MEEFLSATLRGLSFGSVYALLAIGLVLTYKTSGVFHLAYGAQAF
                     VSAVVFYEARVRHEWPIWAAALLAIGVIAPLIGIALEMLLFRYLRRASELAKLVTSIG
                     LLIAIPEIVKLWFEATAGAATSLAPDGEVTYRPFGDVFLNRNDLFNMAAVVIIVVGLT
                     VMFRYTAIGLRMRAVVESSRMTELAGINADRVSVVSWVLSSVMAGLAGVLLLGFSQFA
                     QVRDQDFTVLVTAAIAAAVFAGLTSIPRAFIGGLALGIGQQLLTRYLPTGNVLAQNLR
                     PALPFIALFLVLILMPSLRGRRQATDPLAGVDPPPPAPISTEVGSLLKWGTRGFWTVI
                     GLITAYYIFFSASARWVDIAVRATILSTIFLSIVVITGLAGQISLCQATFAAIGACAT
                     AQLATKQGMSVLVAMLVGAAIAAAVGALLALPALRLGGIFLSLATFAFALFFDSVMVK
                     FDWVGGGVAPIRAPRPQAVGSIDFENDKTFLVLAIVVFTISALLVILVRGATTGRFLR
                     AIQGSEVAASSIGINRNRARITAFAISAAIAGVGGGMLAMYEGRAYINPNFVPFQGLV
                     WVVLVVTLGSRTVEGAVQAGVGFFVFREVFLEQVLPWSVNNVVDPLVPFWHIHMGEPS
                     ATIAFIFFGLGAITYAKHPEGVLEFNKRKSMARIQARLDRR"
BASE COUNT          968 a         1971 c         1983 g         1044 t
ORIGIN      
        1 ccagatgaag acgatcgtcg cgatcacttg ccacacgatg tggatgacgg cgagctcggt
       61 cgcgagccac gatgcaaaga agccgatacc catgagccac tgggggcggc ggatcggcca
      121 cagtgccatg acggtgaagc aggcgcccca gatggagacg agcaggtacc agccggacgc
      181 ggtcacggtt tgaatctacc gtgcggacat tggtgccccg ggcctgggat tccggcgtcg
      241 gtgcacctac catttcgacc gccggccccc tgcaggagga ccccatgcaa cgcctcacgg
      301 gtctcgacgc cgcgttcctc gcgctggaga caccatctgc ccacatgcac gtcatgggcg
      361 tcgcgatcgt cgatccgacg accgcgccgc gcgggttctc ctacgagcgc gtccgcgagg
      421 tcatggcgtc gcggctcgac ttggtcgggc cgttgcggcg tcgcgtcatc gaagtgccgc
      481 tcgggttgca caacccggtc tggatcgagg atcccgactt cgatctcgat ttccacctcc
      541 gccgcgccgc actgccggca ccgggcggtg aagcggagct cgcggcgttc gtcgccgatg
      601 tcgcgggccg cgagctgacc cgtgaccgcc cgttgtggga ggcatgggtc gtcgagggac
      661 tcgagcacgg ctactacgcg ttcgtcgcga aactgcacca ctcgttgatc gacggtgcgt
      721 cgggtgtcga gatcctcgcc gcgttgttcg acatggagcc ggcgcccgag ccgcgcgtcg
      781 cggacatcgt cgaggagtgg catcccgatc acatcccgag cgacttcgag ctcttggcgt
      841 atgccgtgtt gtcgttcgcg caaaggccgc tgtacttcgt caaggccgcg aacaatctcg
      901 gccgaggatt gatgagcgcg gtgcaacgcg cccgcgaaga gtcgctcgac gtcgcgctgc
      961 cgttgactgc gccgcggctc tcgatgaacc gtacgatcac gccccaccgc aaagtcgcgt
     1021 tcgcgtcggt gccgctcgcc gacatcaagg ccgcgaagaa cgcgctcggc gtgaccgtga
     1081 acgacatcgt gcttgccatt gcgtccggtg ccttacgcaa ctacctgcaa cagcgcgaag
     1141 agctgcccga ccgatcactc gtcgccgcca tcccgacgag catccgggcc gaaggtgaac
     1201 gcgagtacgg caaccgtgtg tcggcgatgt ttgcggcgct gccggtcgag ctcgacgacg
     1261 cgatcgatcg tgtgcatgcg gtgcagcgct cgacggccgg cgccaaggga gtgcacgagc
     1321 agatcggctc cacgacgctg caggaatggg cggaggtcgc agccccggcc gtgttctcgc
     1381 gcgcgatgcg gctctacacg cgcctgcgga tcggcgagcg ggtggcgccg gtcatcaacg
     1441 cggtcgtgtc gaacgtgccc ggtccgccgt tcccgctgta cctggccggc gcgcggctgg
     1501 ttgcgctgca cccgctcggg ccgatcttcg acgactgcgg gctgaacctc acggtgatct
     1561 cgtacctcga ccacgtcgac ttcgggttca tcgcgtgccg cgagctgatc ccggatctcg
     1621 aagagctggc cgcggcagtg cccgaagcgc tcgcggagat cgtcaaggcc gcgggctgag
     1681 cgtttcgaac agcgagctct cgcgcaggcc cggttctgcg ccgcgccgga tgaaccactc
     1741 gtagccgaac gactcgcgga acgcgtcgat cggcatctgc aagaagaacg acgcgtcgct
     1801 gatctggctc gcgtgcgcgg ccatcgcggc gcgcttgagc tcgacgaagt cgcgcacgtc
     1861 gaccgccgtc gtcaacgcgg attccggcat cccgaggtgc tcgatgtctt cgggggtcgg
     1921 acgctcctcc tcggagacgt cgtcgaggcc gtccgggatc atctccatca ggcgcaggag
     1981 gtgatcgcgg ttcatcgtcg cttcgagcac gactgttgtc tgtgcgatct ctgctgcgcg
     2041 cacgccgacg cggtgcacct gaatgtgatc ggggtgcccg tagttgccga actcgtcgta
     2101 gacggtcaac acgtccgcgt gctcgccgac caggagcgcg gccagtttct gcgcggcctc
     2161 ttcgagatcg gcatttgcaa atgcaccggt cgcgtgattg gagtcctcgc cggccatgcc
     2221 ggagtcgcgg tagccgagga actcgacgcg atcgacgccc agcaccttgg ccgcggcctg
     2281 ggtctcggcg actcggcgat cggcgagcgt ttcccctggt tcgaggatcg cggggtcgta
     2341 ttcgcccagt tcgccgcggg tcgcgacgac gaggacgacg cgatgacctt gtgcgtgggc
     2401 ctgcgccatg acgccacccg tggcaatggc ctcgtcgtcg ggatgcgcat gaaacacgac
     2461 cagcgtgtac gccacgccgc gatggtggca ggttttcggc cgcgttaccc gaacgtcccg
     2521 atgtggctat tgttcggccg cgaaacctga cgctcgtgtc gggaaacttc ttccgaacct
     2581 gtgagaccgg ggggtccaat gcgcggttcg aagctggtca cgctgggtct tgccctcacg
     2641 ctgctgttgg ccgcgtgcgg gaactccgat gacgacgacg acagcgcggc gcccacgacc
     2701 ggtgcgggcg ggtcgaccac gccgacgacc gcgtccgacc tgaccaaaaa cgtcccgatc
     2761 gacgaggtcg gcgtcaccga cgacgagatc cacgtcaacg tcatcaccgc caagaccaac
     2821 ccggtcggcg gcaagtacgc ggagctcgcc gacggcatca acgcgtactt cgccatgatc
     2881 aacgacggcg gcggcatcta cggccgccag ctgaagatca ccaaggaacg cgacgaccag
     2941 ctcgcgaaca accagcagga gacgcaggcc gcgctcgccg aagaccaggc gttcgcgacg
     3001 ttcgaagcga cgctgctgtt cactggtgcc gacctgctcg acgaagccga ccagccgacg
     3061 ttcatctgga acatcaaccc cgagatggcg ggccacgaca acatcttcgc caacgcgagt
     3121 gcgctgtgct tcggttgcga cggccacatc ctgccgtaca tggccgagaa gcacgaggcg
     3181 acgaagatcg gcatcctcgc gtacggcgtc tccgaagagt cgaagcggtg cgcggccggc
     3241 aaccggtcgt cgatcgagaa gtacggcgcg gcgctcggtc aggaggtcgc gttcttcgac
     3301 gactcgatcg gtttcggtca ggagctcagc gcgcaggtcg cgcagatgaa gtccaacggc
     3361 gtgcagttca tcaccacgtg catggacgcc agcgagacgc tcgtgctcgc caaggagatg
     3421 aagaagcagg gcctcgacgc cgtgcagcac ctgcccaacg ggtacgacca ccagctcatg
     3481 accgacaacg cggccgagtt cgaaggctcg atcgtgatcc cgcagttcct cgccttggag
     3541 accgcggaac cgctccagat ccagaaggac ttcgagaagt ggacgaagga gatcggcgtc
     3601 acgccggtcg agaccacgat cgtcggctgg atcctggccc acgagttcgt cgaagggctg
     3661 aaggcagccg gccccgagtt caacaaggcg aagctcatcg ccgcactgaa cgcgatgacc
     3721 gactacgacg ccgagggcat gatcccgccg atcgactgga cgaagggcca catcgacccg
     3781 tcgacgaacc ccgaggtgcg cggccagctc gagtgtgcga acttcaccgt cgtgcagggc
     3841 ggcaacttcg tgccggagtg ggtggacgat ccggcgaagc cgtggacctg cttcaacgag
     3901 acggatcccg acatcaccaa accgatcccc acgagcttcg ccgaataacg aatcgatagg
     3961 agcccgcgct cgtggaggag tttctcagtg caacactgcg cgggctgagc ttcggcagcg
     4021 tctacgcgct gctcgcgatc ggtctcgtcc tcacctacaa gacgtcaggt gtgttccacc
     4081 tggcgtacgg cgcgcaggcc ttcgtgtccg cggtcgtgtt ctacgaggca cgcgtgcggc
     4141 acgagtggcc gatctgggcg gcggcgttgc tcgcgatcgg cgtcatcgca ccgctcatcg
     4201 gcatcgcgtt ggagatgctg ctcttccggt atctgcggcg agcatccgag ctcgcgaagc
     4261 tggtgacgtc catcggcttg ctgatcgcga tccccgagat cgtcaagctg tggttcgagg
     4321 ccacggccgg cgccgcgacc agcctcgcgc ccgacggcga agtcacgtat cggcccttcg
     4381 gcgacgtctt cttgaaccgc aacgacctct tcaacatggc cgcggttgtc atcatcgtcg
     4441 tcggcttgac ggtgatgttc cgatacacgg cgatcggcct gcggatgcga gcagtggtcg
     4501 agagcagccg catgaccgag ttggccggca tcaacgccga ccgcgtgagc gtcgtgtcgt
     4561 gggtgttgtc gagcgtcatg gccggattgg ccggtgtgtt gttgctcggc ttctcgcagt
     4621 tcgcgcaggt ccgcgaccaa gacttcaccg tgctcgtgac cgccgccatc gcggcggctg
     4681 tcttcgcggg gctgacgagc atcccgcgtg cgttcatcgg cgggctcgcg ctcggtatcg
     4741 gacagcaatt gctcacccgc tacctgccga cgggcaacgt gctcgcgcag aacctgcggc
     4801 ccgcgctgcc gttcatcgcg ctgttcctcg ttctcatctt gatgccgagc ctgcggggcc
     4861 ggcggcaggc gaccgaccca ctggcgggtg tcgacccacc gccgccggcg ccgatctcga
     4921 ccgaggtcgg ttcgctgctg aagtggggca cgcgcgggtt ctggacggtg atcgggttga
     4981 tcaccgcgta ctacatcttc ttctcggcca gtgcgcgctg ggtcgacatc gcggtgcggg
     5041 cgacgatctt gtcgaccatc ttcctgtcga tcgtcgtcat caccgggctc gcgggccaga
     5101 tctcgttgtg ccaagccacc ttcgccgcca tcggcgcgtg tgcgaccgcg cagctcgcga
     5161 cgaagcaagg gatgtcggtg ctcgttgcga tgctcgtggg cgccgcgatc gcggcggccg
     5221 tcggtgcgct cctcgcgttg cccgcactgc gcctgggcgg gatcttcctg tcgttggcga
     5281 cgttcgcgtt cgcactcttc ttcgacagcg tgatggtgaa gttcgattgg gtcggtggcg
     5341 gtgtcgcgcc catccgtgcg ccgcggccgc aggcggtcgg atccatcgac ttcgagaacg
     5401 acaagacgtt cctcgtgctc gcgatcgtcg tgttcacgat ctcggcgctc ctggtcattc
     5461 tcgtgcgcgg cgcgacgacg ggccgattcc tgcgcgcgat acaaggcagc gaggtcgcgg
     5521 catcgtcgat cggcatcaac cgcaaccgcg cccgtatcac cgcgttcgcg atctcggccg
     5581 caatcgcagg cgtcggcggc ggcatgctcg cgatgtacga aggccgcgcc tacatcaacc
     5641 cgaacttcgt gccgttccaa ggcttggtgt gggtcgtgct cgtcgtcacg ctcgggtcgc
     5701 ggaccgtcga aggtgcggtg caggcgggcg tcggcttctt cgtcttccga gaggtgttcc
     5761 tcgaacaggt gctgccgtgg agcgtgaaca atgtcgtcga cccgctcgtg ccgttctggc
     5821 acatccacat gggtgagccg tcggcgacga tcgcgttcat cttcttcggc ctgggtgcga
     5881 tcacgtacgc gaagcacccc gaaggtgtgc tcgagttcaa caagcgcaag tcgatggcca
     5941 ggatccaggc ccgcctcgac cggcgt
//
LOCUS       DASUGA010000190         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_470331, whole genome shotgun sequence.
ACCESSION   DASUGA010000190 DASUGA010000000
VERSION     DASUGA010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470331"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..>2573)
                     /gene="dnaE"
                     /locus_tag="VFX21_03740"
     CDS             complement(216..>2573)
                     /gene="dnaE"
                     /locus_tag="VFX21_03740"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX5095094.1"
                     /translation="PIKYDLLFERFLNPGRKQMPDIDMDFDSRYRGELIRYAARTYGE
                     DHVAQIVTFSTIKARAAVRDSARVLGYPYAVGDKIAKLMPPLIMGRDTPLKACFETMP
                     KHEDGYKMAAELRTLYESDPDAKRVIDVARGLEGLRRQDGIHAAAVVITRDPLTEYLP
                     IQRKPEAGGNIEDAPIVTQYEMHGVEDLGLLKMDFLGLRNLDVLEIALDLIAESTGAR
                     PDIDRVPLDDELTFELLRRGDTVGVFQLEGGPMRALLRSLAPTDFEDVAALIALYRPG
                     PMAQNWHNEYADRKNGRKQVSYDHDDLIEILEPTFGLMVYQEQLMRVSQKLAGYSLEE
                     ADNLRKATGKKIRALIAKERTKFVAGCVANGHAEDWAERYFNTIEPFADYSFNKSHTV
                     GYGYITYQTAWLKANYQVQYLAALLTSVKTNLDKAAVYLSDCRQHDIEVLVPDVNASL
                     SDFSTEGNAIRFGLSAVRNVGEGVATLLIQERAENGPYADIHEFADRVDPAVLNKRTV
                     ESLINGGGFDSLGHPRKGLLTVFEPIIESALRRRRERDAGTMSLFDTGGGADDPSPVF
                     DDRLPIPDMEFDKNERLRAEKEMLGLYVSDHPLMGAERSLRKLVDTSIADLAELEDGA
                     MRTVAGVVTALQRKYTKRGDLMATFVLEDLAAAIEVMVFPKTMLTYGELLTSDAIVTV
                     KGRVDNREDTPKLMAMEITRPEIHIENGRPVRLRVKTSAMTDEKVVRLKEVLSNNPGD
                     SPVLLTLVGPDKETDIRLGDEFHCDDRNGLYAELRILFGADCIVS"
BASE COUNT          402 a          801 c          864 g          506 t
ORIGIN      
        1 tgccacggca ccaactgcaa ctttccgttg ccatgccgtt cccatcgact tgcccccccg
       61 gtggcggacg ctttgccaac gcgcccatct tgcctgcttc ggagcggtgc tgccatctgc
      121 gatcgacact cagccggcgc cttcatcgcc ctcaaacgtg ggcaagttat gtaccgaatc
      181 cgtacacaac ttgcccaaga atggatcgga cgcggctagg acacgatgca gtcggcgccg
      241 aagaggatgc gcagttccgc gtacaggccg ttgcggtcgt cgcagtggaa ctcgtcgccg
      301 aggcggatgt cggtttcctt gtcgggaccg accagcgtca gcagcactgg actatcgccg
      361 gggttgttgg agagcacttc cttcaaccgc acgaccttct cgtcggtcat cgcgctcgtc
      421 ttcacacgca gccgcaccgg gcggccgttc tcgatatgga tctcgggccg cgtgatctcc
      481 atcgccatga gcttgggcgt gtcttcacga ttgtcgaccc ggcccttgac ggtgacaatc
      541 gcgtcggagg tcagcagctc gccgtaggtg agcatggtct tcgggaagac catcacttcg
      601 attgcggcgg cgaggtcttc gagcacgaac gtcgccatga ggtcgccgcg tttggtgtac
      661 ttgcgctgca acgccgtgac cacacccgcg accgtgcgca tcgcgccgtc ttcgagttcg
      721 gccagatccg caatcgaggt gtcgaccagc ttgcgcaggg agcgctcggc acccatgagc
      781 ggatggtcgg acacgtacaa gccgagcatt tctttctcgg cccgcaggcg ttcgttcttg
      841 tcgaactcca tgtcgggaat cgggaggcgg tcgtcgaata ccggtgaggg atcgtcggcg
      901 ccaccgccgg tgtcgaacaa gctcatcgtg cccgcgtcgc gctcgcgccg gcggcgcagc
      961 gccgactcga tgatcggttc gaagaccgtg agcaaaccct tgcgggggtg cccgagcgag
     1021 tcgaagccgc cgccgttgat caacgattcg accgtgcgct tgttcagcac cgccgggtcg
     1081 acgcggtcgg cgaactcgtg gatgtcggcg tagggaccgt tctcggcgcg ctcttggatg
     1141 agcagcgtcg caacgccctc gccgacgttg cgaacggccg agagtccgaa gcggatcgca
     1201 ttgccttcgg tagaaaaatc ggaaagcgat gcgttgacat cgggaacgag cacttcgatg
     1261 tcgtgctggc ggcagtccga caagtacacc gccgccttgt cgaggttcgt cttgacgctc
     1321 gtgagtagcg cagcgaggta ctgcacctga tagttcgcct tcagccacgc ggtttggtac
     1381 gtgatgtagc cgtaaccgac cgtgtgcgac ttgttgaacg agtagtcggc gaatggttcg
     1441 atcgtgttga aatagcgctc ggcccagtct tccgcatggc cgttcgcgac acagccggcg
     1501 acgaacttgg tgcgttcctt cgcgatcagc gcgcggatct tcttgcccgt cgccttgcgc
     1561 aggttgtcgg cctcttcgag cgagtaaccc gcgagcttct gtgacacacg catgagctgt
     1621 tcttggtaga ccatcaaccc gaacgtcggc tcgaggatct cgatgaggtc gtcgtggtcg
     1681 tagctgacct gcttgcggcc gttcttgcgg tcggcgtact cgttgtgcca gttctgcgcc
     1741 atcgggcccg gtcggtagag ggcgatgagc gcggcgacgt cttcgaagtc ggtgggcgcg
     1801 agcgaacgca gcagcgcgcg catcggccct ccttcgagct ggaacacgcc cacggtgtcg
     1861 ccccgacgca gcagctcgaa cgtgagctcg tcgtcgagcg ggacgcggtc gatgtcgggg
     1921 cgcgcgccgg tgctctcggc gatgaggtcg agggcgatct cgaggacgtc gaggttgcgc
     1981 agccccagga agtccatctt gagcagcccg aggtcttcga ccccgtgcat ctcgtactgg
     2041 gtgacgatcg gggcgtcttc gatgttccct cccgcctccg gcttgcgctg gatcgggagg
     2101 tactcggtga gcggatcgcg cgtgatcacg acggccgcgg cgtggatgcc gtcctggcgg
     2161 cgcagcccct cgaggccgcg ggcgacgtcg atcacgcgct tggcgtcggg atcgctctcg
     2221 tagagcgtgc gcagctcggc ggccatcttg tagccgtctt cgtgcttggg catcgtctcg
     2281 aagcacgcct tcagcggcgt gtcgcggccc atgatgagcg gcggcatgag cttggcgatc
     2341 ttgtcgccga ccgcgtaggg gtagccgagc acgcgcgccg agtcgcgcac cgcggcgcgg
     2401 gccttgatgg tggagaaggt gacgatctgc gcgacgtggt cctcgccgta ggtgcgcgcc
     2461 gcgtagcgga tcagctcgcc gcggtagcgc gagtcgaagt ccatgtcgat gtcgggcatc
     2521 tgcttgcggc ccgggttgag gaaccgctcg aacagcaggt cgtacttgat ggg
//
LOCUS       DASUGA010000191         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_729899, whole genome shotgun sequence.
ACCESSION   DASUGA010000191 DASUGA010000000
VERSION     DASUGA010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729899"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VFX21_03745"
     CDS             <1..598
                     /locus_tag="VFX21_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095095.1"
                     /translation="RTVLWIGSPPLRSDKSGGAEELDRVMREEAAKRRPQVLYVDAYK
                     MFGSADGDYTDTLEDDNGKSIRVRIGDGVHLTPAGAEYLANAVFALLDARWKINEQSD
                     PEHRIGYTILHGGESTDSGSGSSSGNSGKRSAATTTTEPGDDGDGGTGSSTSSPPTSD
                     GSPVSTTPPSTTETSPTTSTAPTTAGGSTPTSGQPPTT"
     gene            632..2254
                     /locus_tag="VFX21_03750"
     CDS             632..2254
                     /locus_tag="VFX21_03750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5095096.1"
                     /translation="MTDRLVAARAATKILAEAVDQASRHLAELARDGDKISVPKLDEH
                     QIFAYDLAHVAAAAEASRVMCEYAEHGELESMLARAFVADAVADVVGRILGRTSEWQI
                     DPALFSPAGPFVEEHRAPAFLEALAEQCAKHGTGPTHLSDDFELVADTFHRFAEDKIR
                     PVAEHVHRTNADVPEEIIAGLAEIGGFGLSVPEEYGGFATGGESDYIGMVVSTEELSR
                     GSLGIGGSLITRPEILTRALVAGGTEEQKQQWLPRIASGEVMVGIMVTEPDYGSDVGS
                     VKMTATPTDGGWLMNGVKTWATFAARANIGMLLARTNPDRSLTHKGLSVFVIEKPQAE
                     GHSFAFEDDRGGKIEGRAIDTIGYRGMHSYEVAFDNWFVAAENLIGGDAGLGRGFYLQ
                     MAGFENGRLQTAARAVGLMQAAFEAGLAYAQDRKIFGQAVFDYQLSKAKLARMATLIQ
                     TGRQFSYDVARKMAAGGGTLEAAMVKAYVCRAAEWVTREAMQLHGGMGYAEEFAVSRY
                     FVDARVLSIFEGADETLCLRLITRRLTERAIA"
BASE COUNT          408 a          803 c          814 g          338 t
ORIGIN      
        1 ccgaaccgtc ctctggatcg gatcgccacc actgcggagc gacaagtcgg gcggcgcgga
       61 ggaactcgac cgcgtcatgc gcgaagaagc ggcgaagcga cgcccgcagg tgctgtacgt
      121 cgacgcgtac aagatgttcg gcagcgcgga cggcgactac accgacacgc tcgaggacga
      181 caacggcaag agcattcgcg tccgcatcgg cgacggcgtg cacttgacgc cggccggcgc
      241 cgagtatctc gcgaacgcgg tctttgcact gttggatgcg cgctggaaga tcaacgagca
      301 atccgacccc gagcaccgca tcggctacac gatcctgcac ggcggcgaga gcaccgactc
      361 gggcagcggc agcagcagcg gcaacagtgg caagcgctcc gccgcgacca cgacgacgga
      421 gcccggcgac gatggcgacg gcggcaccgg ttcgtcgacg agctcgccgc cgacgagcga
      481 cgggtcgccg gtgtcgacga ccccgcccag cacgaccgag acgagcccga ccacatcgac
      541 ggcaccgacg acggcgggcg ggtcgacgcc gacctcgggc cagccgccga cgacctgagt
      601 tcttttggag ctgctgggta acttgcccgg catgactgac cgactcgttg cagcgcgcgc
      661 ggccacgaag atcctggccg aggccgtcga ccaggcctcg cggcacttgg ccgagctcgc
      721 ccgcgacggc gacaagatct cggtgcccaa gctcgacgag caccagatct tcgcctacga
      781 cctcgcacac gttgccgccg cggcggaagc gtctcgcgtc atgtgcgaat acgccgagca
      841 cggtgagctg gagtcgatgc tggcgcgggc gttcgtcgcg gacgcggtgg ccgacgtcgt
      901 cggtcgcatc ctcggccgca catcggagtg gcagatcgat cccgcgctgt tctcgcccgc
      961 cggaccgttc gtcgaagagc accgcgcgcc cgcgttcctc gaagcgctcg ccgagcagtg
     1021 cgcgaagcac ggcaccggcc cgacgcatct ctccgacgac ttcgagctcg tcgccgacac
     1081 gttccatcgc ttcgccgagg acaagatccg gccggtcgcc gagcacgtgc accgcacgaa
     1141 cgcagacgta cccgaagaga tcatcgcggg tctcgcggag atcggcggct tcgggctgtc
     1201 ggtgcccgag gagtacggcg gcttcgcgac cggcggcgaa tccgactaca tcggcatggt
     1261 cgtgtcgacc gaagagctgt cgcgcggttc gctcggcatc ggcgggtcgc tcatcacacg
     1321 tcccgagatc ctcacccgcg cgctcgtcgc gggtgggacc gaggaacaga agcagcagtg
     1381 gctgccgcgc atcgcgagcg gcgaggtcat ggtcggcatc atggtcaccg agcccgacta
     1441 cggctccgac gtgggcagcg tgaagatgac cgcgacgccc accgacggcg gctggttgat
     1501 gaacggcgtg aagacgtggg ccacgttcgc ggcgcgcgcc aacatcggca tgttgctcgc
     1561 gcgcacgaac ccggatcgct cgctcacgca caaggggttg tcggtcttcg tcatcgagaa
     1621 gccgcaagcc gaagggcact cgttcgcgtt cgaagacgac cgcggcggca agatcgaagg
     1681 gcgcgcgatc gacacgatcg gctaccgcgg catgcactcg tacgaggtcg cgttcgacaa
     1741 ctggttcgtg gcggccgaga acctcatcgg tggtgacgcg ggactcggcc ggggcttcta
     1801 cctacagatg gccggcttcg agaacggtcg gctgcagacc gcagcgcgcg ccgtcggact
     1861 gatgcaggcc gcgttcgaag cgggtctcgc gtacgcgcag gaccgcaaga tcttcggcca
     1921 ggccgtgttc gactaccagc tgtcgaaggc gaagctggcc cggatggcga cgctcatcca
     1981 gaccggtcgc cagttctcct acgacgtcgc ccgcaagatg gccgcgggtg ggggcacgct
     2041 cgaggccgcg atggtgaagg cctacgtctg ccgggcggcg gagtgggtca cccgcgaagc
     2101 gatgcagctg cacggcggca tgggctacgc cgaggagttc gcggtgtccc gctacttcgt
     2161 cgacgcccgc gtgctgtcga tcttcgaggg cgccgacgag accctgtgcc tccgcctgat
     2221 cacccgccgc ctcacggaac gcgcgatcgc ctgagcggcg gtaggtcgcg cggcccaact
     2281 tttggaagat tcacccgtga cctttccatc gtggacaaaa ggggcgtata ttccgacgcc
     2341 tccggcccat tggcgctgag tgg
//
LOCUS       DASUGA010000192         2163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2015825, whole genome shotgun sequence.
ACCESSION   DASUGA010000192 DASUGA010000000
VERSION     DASUGA010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2015825"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1650
                     /locus_tag="VFX21_03755"
     CDS             <1..1650
                     /locus_tag="VFX21_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX5095097.1"
                     /translation="LALHVAFSLGASWVDYDGDGDLDLYVVTGFSANNENVLYRNDQG
                     TLVRVTGVPLVEDAADSPCSAWGDYDNDGAIDAFVSNLVASGGMLFHGGVLDTTAGVT
                     STALKGTGCAWGDYDNDGRLDLVVSALFGQGNIVTGNRLFHGGGNGTFTEVTVGPMVT
                     TQDTHHHPTWADYDGDGDLDLFFATGPVGSTDTDRMYRNLLRETGAATFAAITTAPIA
                     TDARDSQQLSWVDYDDDGDLDLYAVNYTSLGNQLYRNDGGGAFTKITTGTIVTDTGAN
                     HGVVWGDFDLDGDQDAYVARDNTQSNRYYRNEGNGTFTRISAGAHVTELRSNYGAAAG
                     DYDGDGDLDLFVPTARSEGPSLLFRNDLASGNHWAAFRLEGTVANRTGLGAKVRVRAV
                     IAGVAKWQMRELRNATCYGGHDALEAHFGLGDAATIDSVRVEWPGGTVDTWTNLPADT
                     RRELVEGATVLGVPPAPKRFGLSTAPNPSAGSVRLSYSLPRPGSLAVFDARGRVVHAR
                     PLASGTMSVTIPSNVLPAAGVYMAVVRADGLREATRFVHLK"
     gene            1659..>2163
                     /locus_tag="VFX21_03760"
     CDS             1659..>2163
                     /locus_tag="VFX21_03760"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5095098.1"
                     /translation="MLSLVLALSLVAPPADSIAARADRYLAARTALGAFSGAAVIARG
                     GVPVYAGGHGYADFARKTPFTPDTRIPIASLSKMFTAMAALRLEATGKLKLSDTLGTP
                     AAWRSITIEQLIQHSSGIPDYEESLGMATPAYFAEMTRADAAARQVAWAKKLPLSFAP
                     GTKMHYSN"
BASE COUNT          335 a          777 c          726 g          325 t
ORIGIN      
        1 ctggcccttc acgtcgcgtt ctcgctcggc gcgtcgtggg tcgactacga cggtgacggt
       61 gacctcgacc tctacgtcgt cacgggcttc agcgcgaaca acgagaacgt cctctatcgc
      121 aacgaccagg gaacgctggt gcgggtgacc ggcgtgccgc tggtcgagga cgcggccgac
      181 agtccctgca gcgcatgggg cgactatgac aacgacggcg cgatcgacgc gttcgtctcc
      241 aacctggtcg cttcaggcgg gatgttgttt cacggcggtg tcctcgacac gacggcgggc
      301 gtgacctcta ccgcgctcaa gggcacggga tgcgcgtggg gcgactacga caacgacggc
      361 aggctcgacc tggtggtgag cgcgctcttc gggcagggca acatcgtgac cggcaatcgc
      421 ctgtttcacg ggggcggcaa cggcacgttc accgaggtca ccgtgggccc gatggtgacc
      481 acccaggaca cgcaccacca tcccacgtgg gccgactacg acggggacgg cgacctcgac
      541 ctcttcttcg cgaccggccc ggtcggatcg accgacaccg accggatgta ccggaacctg
      601 ctcagggaaa cgggcgccgc gacgttcgcc gcgatcacga ccgcgccgat tgcgaccgac
      661 gcgcgcgatt cgcagcagct gtcgtgggtc gactacgacg atgacggcga cctcgacctc
      721 tacgccgtca attacacgag cctcggtaac cagctctacc ggaacgacgg cggcggtgcg
      781 ttcacgaaga tcacgaccgg cacgatcgtg accgacacgg gcgcgaacca cggcgtcgtc
      841 tggggcgact tcgacctcga cggcgaccag gacgcgtacg tcgcgcgcga caatacccag
      901 tccaatcgct actaccggaa cgagggcaac gggacgttca cgcggatcag cgcgggcgcg
      961 cacgtcaccg agctgcgctc gaactacggc gcggccgccg gcgattacga cggtgacggc
     1021 gacctcgacc tcttcgtgcc gaccgcgcgc agcgaggggc cgagcctcct cttccggaac
     1081 gacctcgcga gcgggaacca ctgggcggcg ttccgcctcg agggcacggt cgcgaaccgc
     1141 accggcctgg gcgcgaaggt gcgcgtgcgc gccgtgatcg cgggcgtcgc gaagtggcag
     1201 atgcgcgagc tccggaacgc gacctgctac ggcgggcacg acgcgctcga ggcgcacttc
     1261 ggcctgggtg acgcggccac gatcgactcg gtgcgcgtcg agtggccagg cggcacggtc
     1321 gacacgtgga cgaacctccc ggccgacacg cgccgcgagc tggtcgaagg cgcgacggtc
     1381 ctgggcgtcc cccccgcacc gaagcgtttt ggcctgtcca cggcaccgaa tccgtccgcc
     1441 gggtcggttc gtctgtccta ctccctcccc cgtccgggat cgctagcggt gttcgatgcc
     1501 cgcgggcggg tcgtccacgc gcgcccgctc gcttccggca cgatgtcggt gacgattcct
     1561 tccaacgttc tgcccgcggc gggtgtctac atggccgtcg tgcgcgcgga cgggctgcgc
     1621 gaggcgacga ggttcgtcca cctcaagtag gccccatcgt gctctctctg gtccttgcgc
     1681 tttccctcgt cgcgccgccc gccgattcga tcgcggcgcg cgccgaccgc tatctcgccg
     1741 cccgcacggc actcggagcg ttctccggcg ccgcggtgat cgcgcgtggc ggcgttcccg
     1801 tgtacgcggg gggccacggg tacgccgact tcgcgcgcaa gaccccgttc acgcccgaca
     1861 cccggatccc gatcgcctcg ctctccaaga tgttcaccgc gatggccgcg ctccgcctcg
     1921 aggcgaccgg gaagctcaag ctgtcggaca cgctcggcac gcccgcggcg tggcgctcga
     1981 tcacgatcga gcagctgatc cagcactcgt ccgggattcc cgattacgag gagtcgctcg
     2041 gcatggcgac gcccgcgtac ttcgcggaga tgacccgcgc cgatgccgcc gcccgccagg
     2101 tcgcctgggc gaagaagctc ccgctgtcgt tcgctcccgg aacgaagatg cactactcga
     2161 aca
//
LOCUS       DASUGA010000193         6400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_738141, whole genome shotgun sequence.
ACCESSION   DASUGA010000193 DASUGA010000000
VERSION     DASUGA010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6400
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738141"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1726
                     /locus_tag="VFX21_03765"
     CDS             <1..1726
                     /locus_tag="VFX21_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX5095099.1"
                     /translation="QNRMAVVEAWTPSGGAIVRGQGYEPVGEIEATDAVRAVLTEAAQ
                     AGLLCSNGRAVEREGIWVAQGDPMEAALHVLALRLDGATDPSERKVVARFPFDARRRR
                     MSVVTEHEVLVKGAPDAVLPCCCDHGSSTRALNDMTARGLRVLAVARRSLRRGELPLQ
                     ASDAERDLELLALLAFEDPPRSHAADAIERCRRAGMRVAMVTGDHPMTALAIAREVGL
                     ARDDTVVVEGHDLPEDDEVLGATVDRDGVVLARITPEDKLRVARALRARGHVVAMTGD
                     GVNDGPALREADIGVAMGSSGTDVAREAADLVLLDDDFATIVAAIEQGRSTFANLRRF
                     LTYHLTDNVAELTPFVIWALSGGRFPLAIGVLQVLALDIGTDTLPAVALGAEQPSLYA
                     LEGPPDRGRLLNGRVARRAFGVLGPAEAIFAMLAFVVSLVASGWRVGTSFPDGPPLLA
                     ASGAAFASIVLGQAANAFACRSSRRPVYALHRPANRFLRIAVAIEIATLALFLWVRPL
                     ADLLDQAAPTAAGLAVALLTPIAVVAADALDKARRRRQRDDPRYDRAVARPAFVPRYG
                     SSTAAPVT"
     gene            complement(1693..2691)
                     /locus_tag="VFX21_03770"
     CDS             complement(1693..2691)
                     /locus_tag="VFX21_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011434731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX5095100.1"
                     /translation="MRAWAISEPGPIATSPLRRVDREPPRVKPTEIRIRVEACGVCRT
                     DLHLAEGDLAPHVPQAVPGHEVVGVVDEAGAGCRQFGVGDRAGIAWLRYTCGQCHACR
                     TGAENLCPNARFTGWDADGGFAEWATIDEAYAYHLPARYDAAHAAPLLCAGIIGYRAL
                     ERASVPPNGRLGIYGFGASAHLTAQVAMARGARVHVMTRSEAAREHARELGVASVAAA
                     DAPPPEPLDAAILFAPVGTLVPPALEALADGGTLAIAGIHLSDIPSLNYDRHLFRERQ
                     IRSVTANTRRDGTEFLALAAKIPLQVRVQPYQLDEADVALRDLAADHVTGAAVLLP"
     gene            2859..3071
                     /locus_tag="VFX21_03775"
     CDS             2859..3071
                     /locus_tag="VFX21_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1918 domain-containing protein"
                     /protein_id="HEX5095101.1"
                     /translation="MRASVGDRIIIKGHHVGEPDRDCEVLEVHGADGEPPWFVRWDDG
                     HEGLYFPGSDAHVQHFVHGKAAGERT"
     gene            3068..3850
                     /locus_tag="VFX21_03780"
     CDS             3068..3850
                     /locus_tag="VFX21_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5095102.1"
                     /translation="MIPRNIVVPLDGSAFAERAIPLAHKLTAQTGGRVTLMRAREDDD
                     PAREYDYLQKAAAEHGPVNVLFVQDRQAAPAISVVAEDDGEHLVCMTSHGRGSLRWAA
                     LGSVTEAVIRESRVPVLVVGRHCVLDAAPFEHAVVALDGSDANDPVVPVAIEWSRELG
                     LTVRLVHVIHPLDVEGAEHPNKAVQAIADRMRESGLTVEALLLRSTIVGAAIADAAAA
                     VPGPLVMMSSHSRTGARRFALGSVAMHAVAMSPAPVLVVHRD"
     gene            3925..4119
                     /locus_tag="VFX21_03785"
     CDS             3925..4119
                     /locus_tag="VFX21_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095103.1"
                     /translation="MTTNAQHQRSGSIAGLTWGVLVVVGLVLLVLHNSPWAAAWVTAA
                     AVSFVAIVGVAFAVRVFHRR"
     gene            complement(4184..5197)
                     /locus_tag="VFX21_03790"
     CDS             complement(4184..5197)
                     /locus_tag="VFX21_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEX5095104.1"
                     /translation="MKVAVVKELGQPLVIEERAIPEPGPGEVRVRIETAGLCHTDIHA
                     AHGDWPVKPAPPFVPGHEGVGIVDAVGPNVRGRSAGDRVAIPWLGWACGTCEHCVGGW
                     ETLCLAQHNTGYSIDGSYAEYVTAAADFVVPVPAGIDPMEAAPLTCAGVTTYKAVKVS
                     GARPSDLVAVFGIGGLGHMAVQYACISGARVAAVDIADDKLEIAHSLGADVLVNAASD
                     DPGTAITALGGADAAVVTAATPRAVAQAYRSLKRGGRLVLVGLPAENHIDLPIFETVL
                     NGITVVGSIVGTRRDLAEVFELHAAGRTHVVYEERKLDEVNDAFTDVLAGRVPARVVF
                     RIS"
     gene            complement(5194..>6400)
                     /locus_tag="VFX21_03795"
     CDS             complement(5194..>6400)
                     /locus_tag="VFX21_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020477959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEX5095105.1"
                     /translation="GEVLVAPMTNPDWVPVLRRAAALVTDSGGMTCHAAIVSRELGVP
                     CVVGTRSATTALRNGQCVTVDGSKGIVVEGAAPQPDVDVRETPAATVGAVEPLATLLY
                     VNLAMADNAERVAALPVDGVGLLRAEFMLTDALGGRHPRALIAAGEQREFVDRMAASL
                     LRITTAFAPRPVVYRTIDFRSNEFSNLQGGHQFEPVEENPMIGYRGCFRYVREPDLFA
                     LECELLARVREQTPNLHVMIPFVRTKWELEACLELMDKSPLGRQRGLRRWVMAEVPSV
                     VYRIPDYAALGIDGVSIGSNDLTQLMLGVDRDSEICAELFDESDAAVLAAIRDIITGA
                     RAAGLTSSLCGQAPSNRPRFAEHLVRFGITSISVNPDAVDQARLVIGAAERRLLLDAA
                     RREVEEVLP"
BASE COUNT          933 a         2204 c         2266 g          997 t
ORIGIN      
        1 gcagaaccgc atggcggtgg tcgaggcatg gacgccgtcg ggcggagcga tcgtacgcgg
       61 tcagggctac gaaccggtcg gggagatcga ggccaccgac gctgtacggg cggtgctcac
      121 cgaagcggca caggccggcc tcttgtgctc gaacgggcgc gccgtggaac gcgagggcat
      181 ctgggtcgcg cagggcgatc cgatggaggc cgcactgcac gtgctcgcgc ttcgcctcga
      241 cggcgcgacc gacccgagcg agcggaaggt ggtcgctcgt ttcccgttcg acgcgcgccg
      301 gcgtcgcatg tcggtcgtca ccgagcacga ggtgctcgtg aaaggtgcgc ctgacgcagt
      361 gctcccgtgc tgttgtgacc atggctcgag cacacgcgcg ctgaacgaca tgacggcccg
      421 cgggctgcgc gtgctcgcgg tggcccgccg gtccctccgc cgcggcgagc ttccgttgca
      481 ggcgtccgac gccgagcgag acctcgaact gctggccctg ctcgcgttcg aggacccgcc
      541 ccgcagccat gccgccgacg ccatcgaacg atgccggcgc gcggggatgc gggtcgcgat
      601 ggtcacgggc gaccatccga tgaccgcgct cgcgatcgct cgcgaggtcg gcctcgcccg
      661 cgacgacacg gtcgtcgtcg agggtcacga ccttcccgaa gacgacgaag tgctcggtgc
      721 gacggtcgat cgcgacggcg tggtgctggc gcgcatcacg ccggaggaca agctgcgcgt
      781 ggcgcgagcg ctgcgcgcac gcggtcacgt cgtcgcgatg accggcgacg gcgtgaacga
      841 cggtcctgcg ctgcgggagg ccgacatcgg tgtcgcgatg ggaagctcgg gaaccgacgt
      901 tgcccgcgag gccgccgatc tggtgctgct cgacgacgac ttcgcgacga tcgtcgcggc
      961 gatcgagcag ggccgatcga ccttcgcgaa tctgcgccgc ttcctcacgt accacctcac
     1021 cgacaacgtc gcggagctga cgccgttcgt gatctgggcg ctgtcgggcg gccgcttccc
     1081 actcgcgatc ggtgtcttgc aagtgctcgc cctcgacatc gggaccgata ccctgcccgc
     1141 cgtcgcactc ggcgccgagc aaccgagcct gtacgcgctc gaaggtccac cggaccgcgg
     1201 tcgtttgctc aacgggcgcg tcgcgcgccg ggcgttcggc gtgttgggcc cggccgaagc
     1261 gatctttgcg atgctcgcct tcgtggtgtc gttggtggcg agcggttggc gagtggggac
     1321 gtcgttcccc gatggcccgc ctctcctcgc cgcatccggc gccgcgttcg cgagcatcgt
     1381 gctgggccag gcggccaatg cgttcgcgtg ccggagcagc cgccgccccg tctatgcgct
     1441 gcaccgtccc gccaaccggt tcctgcgcat cgcggtagcg atcgagatcg cgacgctggc
     1501 gctgttcctg tgggtgcggc cgctcgccga cctgctcgac caggcggcac cgacggccgc
     1561 cgggctcgcg gttgcactcc tcacaccgat tgcagtcgtc gccgccgatg cactcgacaa
     1621 ggcgcggcgc cgacggcaac gtgacgatcc gcggtacgac cgcgcggtgg cgcgtcccgc
     1681 gttcgtcccg cgctatggca gcagcaccgc ggcaccggtg acatgatctg cggccaggtc
     1741 gcgcagcgcg acgtccgctt cgtcgagctg gtacggctgg acgcgcacct gcagcgggat
     1801 cttcgccgcc agcgccagga actccgtgcc gtctcggcgc gtgttggcag tcacactacg
     1861 gatctggcgt tcgcggaaca ggtggcggtc gtagttcagc gacgggatgt cactgaggtg
     1921 gatgcccgcg atcgccagcg tgccgccgtc ggcgagcgcc tcgagcgcgg gcgggacgag
     1981 ggtgccgacc ggcgcgaaca agatggcggc gtcgagcggc tcgggcggtg gagcatcggc
     2041 ggcggcgact gacgcgacac cgagctcgcg cgcgtgctct cgtgcggctt ccgagcgcgt
     2101 catgacgtgc acgcgtgcgc cgcgcgccat cgccacttgg gcggtgagat gtgccgacgc
     2161 gccgaagccg tagatcccga ggcggccgtt cggcggcacg ctcgcgcgct ccaatgctcg
     2221 gtaaccgatg atcccggcgc acagcagtgg cgcggcgtgc gcggcgtcgt agcgcgcagg
     2281 gaggtggtac gcgtacgcct cgtcgatcgt cgcccactcg gcgaagccac cgtcggcgtc
     2341 ccacccggtg aagcgcgcgt tcgggcacag gttctccgcg ccggtacggc acgcgtggca
     2401 ttgaccgcat gtgtaccgga gccacgcgat gcccgcgcgg tcgccgaccc cgaattgccg
     2461 gcaaccggcg ccggcctcgt cgaccacgcc gacgacctcg tggccgggga cggcctgcgg
     2521 cacgtgcggc gccagatcgc cttcggcgag atggagatcg gtgcggcaca cgccgcaggc
     2581 ttcgacccgg atccggatct cggtcggctt cacgcgcgga ggttcgcggt cgacgcgccg
     2641 gaggggactc gtcgcgatcg gtcccggttc cgagattgcc cacgctcgca tgggtggctc
     2701 ctccgttcca caccagtgtc gtcccggagt gcgaagcgcg ccatggacga aggtcacgtt
     2761 gtcggcgaca gggtctgcgt ggaggacctt cgaccctggc gcgattcggt cacggttgga
     2821 gatcatcgga ggggtcgatg cttcgattgg aggacgtcat gcgcgcgagt gtcggtgatc
     2881 gcatcatcat caagggacat cacgtcgggg agcccgatcg cgactgcgaa gtgctcgaag
     2941 tgcacggtgc cgacggcgaa ccgccctggt tcgtccgctg ggacgacggc cacgaaggcc
     3001 tgtacttccc cggcagcgac gcgcacgtac aacacttcgt gcacggcaag gctgccggag
     3061 aacggacgtg attccccgca acatcgtggt accgctcgac ggctcggcgt ttgccgaacg
     3121 cgcgatcccg ctcgcgcaca agctcacggc ccaaaccggc ggccgcgtca ccctcatgcg
     3181 ggcgcgcgag gacgacgacc cggcgcgaga gtacgactat ctccagaagg ccgcggccga
     3241 gcatggtccg gtgaacgtgc tcttcgtcca ggatcggcag gccgcacccg cgatctccgt
     3301 tgtcgccgaa gacgacggcg aacacctcgt ctgcatgacc agccatggtc gaggttcgct
     3361 gcggtgggct gcgctcggca gcgtcaccga ggcagtcatc cgagagtcac gcgtccccgt
     3421 gctggtcgtc ggccgccact gcgtgctcga cgccgcaccc ttcgaacacg cagtcgtggc
     3481 cctcgacgga tcggacgcga atgatcccgt cgttccggtt gcgatcgagt ggtcgcgcga
     3541 gctcggcctg accgtgcgtc tcgtgcacgt gatccacccg ctcgacgtcg agggggcgga
     3601 gcacccgaac aaggcggtgc aggcgatcgc cgatcgcatg cgcgaaagcg ggctgacggt
     3661 cgaggcattg ctgctgcgat ccacgatcgt cggcgccgcg atcgccgatg ccgcggcggc
     3721 cgtccccggt ccgctggtga tgatgtcgag tcacagtcgc accggtgccc gacgcttcgc
     3781 gttgggcagt gtggccatgc acgccgtggc gatgtcgccc gcgcccgtgc tcgtcgtgca
     3841 tcgggattag caaggctcgg tccggacgac aggtcgggac caacggctct atgggcgaga
     3901 cgaacgacgg ccgacaatcg aggcatgaca acgaatgctc aacatcaacg gagcggctcg
     3961 attgccgggt tgacgtgggg cgtgctggtc gtcgtcgggc tcgtgctgct cgtcctgcac
     4021 aactcgccgt gggccgcggc ctgggtgacc gcagccgcag tgtcgttcgt tgcgatcgtc
     4081 ggcgttgcct tcgcggtgcg cgtgttccac cgccggtagg tgaagccgac ggcgctcggt
     4141 ggtcggtact gcaggccacc gagcgcatcc gtcgtcgccg tgctcagctg atccggaaga
     4201 ccacgcgcgc cggcacccgg cccgccagca cgtccgtgaa cgcgtcgttg acctcgtcga
     4261 gcttgcgctc ttcgtacacg acgtgcgtgc gtcctgccgc gtgcagctcg aacacttccg
     4321 caaggtcgcg gcgggtaccg acgatcgaac cgacgaccgt gatgccgttc agcaccgtct
     4381 cgaagatcgg caggtcgatg tggttctcgg cgggcagccc gacgagcacg aggcgaccac
     4441 cgcgcttgag gctccggtac gcctgggcga cggcccgggg ggttgcggcc gtcaccaccg
     4501 cggcgtcggc gccgccgagc gcggtgatcg cggttccggg gtcgtcgctc gcggcgttga
     4561 cgagcacgtc cgcgccgagg ctgtgcgcga tctcgagctt gtcgtccgcg atgtcgaccg
     4621 cagcgacgcg ggcaccactg atgcacgcgt actgcaccgc catgtgcccg agacctccga
     4681 tgccgaagac ggcgacgaga tcggagggcc gcgcgccgga gaccttcacg gccttgtagg
     4741 tcgtgacgcc cgcgcaggtg aggggtgcgg cttccatcgg atcgatgccc gcgggcaccg
     4801 gcacgacgaa gtccgcggcc gcggtcacgt attcggcata tgacccgtcg atcgagtacc
     4861 cggtgttgtg ttgcgccagg cacagtgtct cccagccgcc gacgcagtgc tcgcaggtcc
     4921 cgcatgccca tccgagccac gggattgcga ctcgatcgcc ggcactgcgg ccgcggacgt
     4981 tcgggccgac cgcgtcgacg atgccgacac cctcgtgacc gggcacgaac ggcggtgcgg
     5041 gcttgacggg ccagtcgccg tgcgcggcat ggatgtcggt gtggcacagg cccgcggtct
     5101 cgatccggac gcgcacttcg ccgggacccg gctcggggat cgcacgttcc tcgatgacga
     5161 ggggctgacc cagctctttg acgactgcga ccttcatggc aatacctcct cgacttcgcg
     5221 ccgcgccgcg tcgagcagca ggcgacgttc ggcggctccg atgacgagtc gcgcctggtc
     5281 gaccgcgtcg gggttcaccg agatcgatgt gatgccgaag cgcacgaggt gctcggcgaa
     5341 gcgcggccgg ttcgacgggg cctggccgca caacgacgat gtgagaccgg ctgcgcgcgc
     5401 gcccgtgatg atgtctctga tcgcggcgag caccgccgcg tcggattcgt cgaacagctc
     5461 ggcgcaaatc tccgagtcac ggtcgacacc gagcatcagc tgcgtgagat cgttcgaacc
     5521 gatcgagacg ccgtcgatgc ccaacgccgc gtagtcaggg atgcggtaca cgaccgacgg
     5581 gacctcggcc atcacccacc gccgcaaacc gcgctggcgc ccgagcggac tcttgtccat
     5641 cagctcgagg cacgcctcga gctcccactt cgtgcgcacg aaggggatca tgacgtgcag
     5701 attcggtgtc tgctcccgca cacgcgccaa tagctcgcac tcgagtgcga acaagtccgg
     5761 ttcgcgcacg tagcggaagc agccgcggta gccgatcatc gggttctcct cgaccggctc
     5821 gaactggtgc ccgccttgga ggttgctgaa ctcgttgctg cggaagtcga tcgttcggta
     5881 cacgaccggg cgcggcgcga acgcggtcgt gatgcgcaag agcgacgccg ccatcctgtc
     5941 gacgaactcg cgctgctcgc ccgcggcgat gagggctctc gggtggcgcc cgccgagcgc
     6001 gtcggtgagc atgaactcgg cgcgcaacaa gccgacaccg tcgacgggta gcgcggcgac
     6061 gcgctcggcg ttgtcggcca tcgcgagatt gacgtacaga agcgtcgcaa gcggttcgac
     6121 ggcaccgacc gtcgccgcgg gcgtctcgcg cacgtcgacg tccggttgcg gggccgcgcc
     6181 ttcgacgacg atgcctttgc tgccgtcgac cgtgacgcac tgcccgtttc gcagtgccgt
     6241 tgtcgccgaa cgcgtgccga cgacgcacgg gacgcccagc tcgcggctga cgatggcggc
     6301 gtgacacgtc atcccgccgc tgtcggtgac gagtgccgcc gcccgccgca acaccggcac
     6361 ccagtccggg ttcgtcatcg gtgccacgag cacttcgccg
//
LOCUS       DASUGA010000194         6123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_903309, whole genome shotgun sequence.
ACCESSION   DASUGA010000194 DASUGA010000000
VERSION     DASUGA010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903309"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..970
                     /locus_tag="VFX21_03800"
     CDS             35..970
                     /locus_tag="VFX21_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095106.1"
                     /translation="MRGEADNEIARISEGKDGIFTLADARSCDFSDRQMDGRVGTMWV
                     PLYDGVYRMAGAPLTWRGEMRAACSAGTFPIAVSHRCAGEIYRVPWRRPDVEITTGRW
                     RRTKYSGLIVHESTRFDERDIDTETHGFPVVRAEFLLLQLAALRPNPNYLEAVIHAVR
                     RGRYVTYESTMETFERHARRGVPGVRALREALGRWRPGEAATQSDPELDLIQILRKHG
                     FDPVPQYRVYDEHGIFVAQVDAGLPQWKITVEYESDQEHLDEFQIAHDDKRRNDIVTA
                     GYKPLAARKHDLRRGALDLIEQIETIARRAERKPA"
     gene            complement(1072..2466)
                     /locus_tag="VFX21_03805"
     CDS             complement(1072..2466)
                     /locus_tag="VFX21_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5095107.1"
                     /translation="MVLPATAVETLAADFLGPLVTPDSPTYDEDRKIWNGHIDRRPAL
                     IARCQGVADVIAAVRFAREQGVAASVRGGGHAVAGHALVDDGIVIDLSLMTGARVDPM
                     AKTIRIQGGALNEHLDRETQAFGLAATGGIVSHTGVGGLTLGGGIGHLMRKFGLSIDA
                     LRSCDVVTADGEFVVANERENADLFWALRGGGGNFGIVTNFEFALQPLGPQVYCGMVM
                     WPMDEAPAVLSFLRDFIADAPDEIGLMGNLRLAPPLAIVPEAFHGKPIVALVCTYAGA
                     VEDGERAFAPLQSLPAPALNALVPKPYTTHQKMFNAALPHGRHYYWRSHRLGPLTDDV
                     QRVIIDHASTITSGMSSIPLFTFGGAVARVAEDATPFPHRSAAHDINIVASWLPDDPE
                     PDRHIEWVRETCDAIAPHSLGVYVNFTSDDSPERVKQAAYSPTQWTRLTGIKAKYDPT
                     NFFKANANIPPNGA"
     gene            2553..3785
                     /locus_tag="VFX21_03810"
     CDS             2553..3785
                     /locus_tag="VFX21_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007383623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5095108.1"
                     /translation="MELPKIVSVDDHVVEPPHVWQTWLPEKWRERGPRVERKKWGPFV
                     HKPGARYTNTEDPNGQWGDAWYYDGKLIYVQKRFVAIPQEAIVDRADGGVESFDRTKM
                     VMEAVTYDEMRKGCWDRDARVKDMELNWVDGSLPFPTFPRFCGQTFYEGDDKELGLAC
                     VQAYNDWMIQEWCGPSNGYNIPLCLIPLWDVDLAAAEVQRVADQGCRAICFSELPHHL
                     KLPTIHSGGWDKMFQVCNDTGVTLCMHIGSSSTNPAASPDAPGGVGGTLAFNNSMASL
                     ADWLFSGKLIEYPKLKLAYSEGQIGWIPYALERADTVWEHHDSWQHSKERIPEPPSSY
                     YYGRVFGCFTADHHGLHSLKEVGEDNICFETDYPHTDTTWPHSKEYAEKMLADFDETV
                     AYKVLRGNAIKMLELDRV"
     gene            complement(3799..4842)
                     /locus_tag="VFX21_03815"
     CDS             complement(3799..4842)
                     /locus_tag="VFX21_03815"
                     /inference="COORDINATES: protein motif:HMM:NF014954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095109.1"
                     /translation="MIPTCVGDLTPEWCTEALGREITAVDAAPIGVGVGLVGQLFRLH
                     LSSPAGDRTVIAKLAAAGEESRFVATILNMYGREHGFYTEIAERAAVSAPDCYYCAHD
                     PETQDTVLLLEDVSARGTAFDQISGCSVADAEPALRALARFHAGFWDDASLDAHAWLL
                     KLCDDPYPGAVSMAYSGGWARMPEFYPDLMTPEVRRFGDAYNDRIPALFAKLCEGPLV
                     MSHADWRLDNLFFAPDGDVIAIDWQLIDRSVGVRDVSYLVTQSVNLANASECRGALEI
                     YLDALGEHGVDADRGWARAMYRYATAFGFVYPVVAAGSLTVEDPRHLELCRMLLERCI
                     AAIAALDAWDLPL"
     gene            complement(4839..5639)
                     /locus_tag="VFX21_03820"
     CDS             complement(4839..5639)
                     /locus_tag="VFX21_03820"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5095110.1"
                     /translation="MKIDGARVVVTGASRGIGEALTHALASRGAHVVLVARDADALEK
                     LAANTGGEAFPADLTKTREIADLVDRIEAGGPIDLLVNNAGVDLVGELTEMRADQIEQ
                     LFTLNLVAPVLLSRAVLPGMRARKRGHIVNVSSLAATNAVPGLAPYSASKAGLSHFTA
                     GLRAECKGTPIKTTLVEIGTVVTSMLDNVESHDPTKRAFKRMKHLQLLPDLEVETVVG
                     AIVHALEHDRRHVRLPYRDALFPMIVETPRRLTEVLLLGVKAQRKGHR"
     gene            5726..>6123
                     /locus_tag="VFX21_03825"
     CDS             5726..>6123
                     /locus_tag="VFX21_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 family protein"
                     /protein_id="HEX5095111.1"
                     /translation="MDSFRGWPADALAFYAGLEADNSRTYWQAHKATYDDSVKAPFLA
                     LSEVVAKEFGPLRLFRPHRDTRFSKDKTPYKTAQGAVTEGRGGTMYYVALSAEGLYAG
                     SGYYMFGPDQLERWRAAVADPRSGPALERAL"
BASE COUNT         1024 a         2036 c         2117 g          946 t
ORIGIN      
        1 acgcgctgtc tcacccgcgc gcgagcctgc ggctatgcgg ggtgaagcag acaacgagat
       61 cgcgcggatc agcgaaggca aggacggcat cttcacgctc gccgatgcgc ggtcatgcga
      121 cttcagcgat cggcaaatgg acggacgcgt cgggaccatg tgggtgccgt tgtacgacgg
      181 cgtctaccga atggcgggcg cgccgctgac gtggcgcggt gagatgcgcg cggcgtgttc
      241 cgccggtacc tttccgatcg cggtgtcgca ccgctgcgcg ggcgagatct atcgggtgcc
      301 atggcggcga cccgacgtcg agatcacgac cggccgttgg cggaggacga agtactcggg
      361 gctcatcgtc cacgagtcga cccgtttcga cgagcgtgac atcgacacgg agacgcacgg
      421 ctttccggtg gttcgggccg agttcttgct cctgcagctc gccgcactgc gaccgaatcc
      481 gaactacctc gaggccgtga tccatgcggt ccggcggggc cggtacgtga cgtacgagtc
      541 gacgatggag acgttcgaac gccacgctcg acgcggtgtt cccggcgtac gagcgttgcg
      601 ggaagcgctg ggccgctggc gccctggtga agcggcgacc cagagcgatc cggagctcga
      661 cctgatccag atcctgcgca agcacgggtt cgaccccgtg ccgcagtacc gggtctacga
      721 cgagcacggg atcttcgtcg cgcaagtcga cgcgggtctc ccgcaatgga agatcacggt
      781 cgaatacgag agtgaccagg agcacctcga cgagttccag atcgcgcacg acgacaagcg
      841 ccgcaacgac atcgtcaccg ccggctacaa accgcttgcg gcccgcaagc acgacctccg
      901 gcgcggcgcg cttgatctga tcgagcagat cgagaccatc gcccgccgcg ccgagcgcaa
      961 accggcgtga ctcaaccacc ggggcggtgg ttgagcggcg caggtacgaa cgtgtgcgcg
     1021 tacctgcgtc cctgaaccac ccgcgcggtg gttgcgcggc gcaggtacgc gctatgcgcc
     1081 gttgggtggg atgttggcgt tggccttgaa gaagttggtg gggtcgtact tggccttgat
     1141 gccggtgagg cgtgtccact gcgttggcga ataggcggcc tgcttcacgc gttcggggct
     1201 gtcgtcgctc gtgaagttca cgtacacacc gaggctgtgc ggtgcgatcg cgtcgcacgt
     1261 ctcgcgtacc cactcgatgt gacggtcggg ctcagggtcg tcgggcagcc acgacgcgac
     1321 gatgttgatg tcgtgcgcgg cgctgcggtg cgggaagggc gtcgcgtcct ccgcgacgcg
     1381 cgcgaccgcg ccgccgaacg tgaacagcgg gatgctcgac atgccggacg tgatcgtcga
     1441 cgcgtggtcg atgatgacgc gttgcacgtc gtcggtgagc gggccgagcc ggtgtgagcg
     1501 ccagtagtag tggcggccgt ggggcagcgc ggcgttgaac atcttctggt gcgtcgtgta
     1561 cggcttcggc accagcgcat tcagggcggg ggcgggcaac gactgcaacg gcgcgaacgc
     1621 gcgctcgccg tcctcgaccg cacccgcgta ggtgcacacc agcgcgacga tcggcttccc
     1681 gtggaacgct tcgggcacga tcgccaacgg cggtgcgagg cgcaggttgc ccatcaggcc
     1741 gatctcgtcg ggcgcgtccg cgatgaagtc gcgcaggaac gacaggacgg ccggtgcctc
     1801 gtccatcggc cacatcacca tgccgcagta cacctgcggc ccgagcggct ggagcgcgaa
     1861 ctcgaagttg gtgacgatgc cgaagttgcc accgccgccg cgcagcgccc agaagagatc
     1921 cgcattctcg cgctcgttcg cgacgacgaa ctcgccgtcg gcggtcacca catcgcacga
     1981 ccgcaacgcg tcgatcgaca aaccgaactt gcgcatcagg tgtccgatgc cgccgccgag
     2041 tgtcaggccg ccgacgccgg tgtggctcac gatcccgccc gtcgccgcga gcccgaacgc
     2101 ctgcgtctcg cggtcgaggt gttcgttgag cgcgccgcct tggatgcgga tggtcttcgc
     2161 catcgggtcg acgcgcgcac cggtcatcaa cgagaggtcg atgacgatgc cgtcgtcgac
     2221 gagcgcgtga ccggcgaccg catggccgcc gccgcgcacc gatgccgcga cgccttgttc
     2281 gcgcgcgaat cgcaccgcgg cgatgacgtc ggcgacgccc tggcagcgcg cgatcagcgc
     2341 gggccggcgg tcgatgtggc cgttccagat cttgcggtct tcgtcgtacg tcggcgagtc
     2401 gggcgtgacc agcgggccga ggaagtcggc ggccagcgtt tcgacagcag ttgcaggcag
     2461 caccatgact cgggagcgta cgacccgcat cgcccggcga acagggcccg agtaacctga
     2521 cacgggcgtc aggtcggccg cggaggagcg ccatggaact gccgaagatc gtcagcgtcg
     2581 atgaccacgt cgtcgaaccg ccgcacgtct ggcagacgtg gctgcccgag aagtggcggg
     2641 agcggggccc gcgcgtcgag cgcaagaagt ggggcccgtt cgtgcacaag cccggtgcgc
     2701 gctacacgaa caccgaggac ccgaacggtc agtggggcga cgcctggtac tacgacggca
     2761 agctcatcta cgtccagaag cggttcgtcg cgatccccca ggaggcgatc gtcgaccggg
     2821 ccgacggcgg cgtcgagtcg ttcgaccgca cgaagatggt gatggaagcg gtcacctacg
     2881 acgagatgcg caagggctgc tgggaccgcg acgcacgcgt caaggacatg gagctcaact
     2941 gggtcgacgg ctcgctgccg ttcccgacct tcccgcgctt ttgcggtcag accttctacg
     3001 aaggtgacga caaggagctc ggtctcgcgt gcgtccaggc gtacaacgac tggatgatcc
     3061 aagagtggtg cgggccgtcg aacggctaca acatcccgct ctgcctgatc ccgttgtggg
     3121 acgtcgacct cgccgctgcc gaagtgcagc gcgtcgccga ccagggttgc cgcgccatct
     3181 gcttcagcga gttgccacac cacttgaagc tgccgacgat ccactccggc ggctgggaca
     3241 agatgttcca ggtgtgcaac gacaccggtg tcacgctgtg catgcatatc ggctcgtcgt
     3301 cgacgaaccc ggccgcgtcg cccgacgccc ccggcggcgt cggcggcacg ctcgcgttca
     3361 acaactcgat ggcgtcgctc gccgactggt tgttcagcgg caagctcatc gagtacccga
     3421 agctaaaact cgcgtactcc gaaggccaga tcggctggat cccgtacgcc ctcgagcgcg
     3481 ccgacacggt gtgggagcac cacgacagct ggcagcactc gaaggagcgc atccccgaac
     3541 cgccgtcgag ctactactac ggccgtgtgt tcgggtgctt caccgcggac caccatggtt
     3601 tgcactcgct gaaagaggtc ggcgaggaca acatctgctt cgaaaccgac tacccgcaca
     3661 ccgacacgac gtggccgcat tcgaaggagt acgcggagaa gatgctcgcc gacttcgacg
     3721 agacggtcgc ctacaaggtg ctgcgcggca acgcgatcaa gatgctggag ctcgaccggg
     3781 tctgacggcg ctgaccgatc agagcgggag atcccacgcg tcgagcgcgg cgatcgcggc
     3841 gatgcaccgt tcgagcagca tccggcacag ttcgaggtga cgcgggtctt ccaccgtcaa
     3901 gctgccggcc gcgacgacgg gatagacgaa gccgaatgcc gtcgcgtagc ggtacatcgc
     3961 acgggcccat ccccggtcgg cgtcgacgcc gtgttcgccg agcgcgtcga ggtagatctc
     4021 gagcgcgccg cggcactcgg acgcgttcgc gagattgacg ctctgcgtca cgagatacga
     4081 cacgtcgcgg acgccgaccg agcggtcgat gagctgccag tcgatcgcga tgacgtcgcc
     4141 gtcgggtgcg aagaagaggt tgtcgagccg ccagtccgcg tgtgacatca cgagcgggcc
     4201 ctcgcacaac ttcgcgaaca acgcgggaat tcggtcgttg tacgcgtcgc cgaacctccg
     4261 cacctccggt gtcatcaggt cgggatagaa ctcgggcatg cgggcccatc cgccgctgta
     4321 cgccatcgac accgcgccgg ggtacgggtc gtcgcacagc ttcagcaacc acgcgtgagc
     4381 gtcgagtgat gcgtcgtccc agaacccggc gtgaaagcgc gccaacgcgc gcagcgcggg
     4441 ttccgcatcc gcgacgctgc agcccgagat ctggtcgaac gcggtgccgc gcgcgctgac
     4501 gtcctcgagc aggagcacgg tgtcctgcgt ctcggggtcg tgcgcgcagt agtagcaatc
     4561 gggggcggac acggccgccc gttcggcgat ctcggtgtag aagccgtgtt cgcggccgta
     4621 catgttgagg atcgtcgcga cgaagcggct ctcctcgccg gccgcggcca gcttcgcgat
     4681 cacggtgcgg tcaccggcag gggacgacag gtgcagccgg aacaactggc cgacgagccc
     4741 caccccgacg ccgatcggcg cggcgtcgac cgcggtgatc tcgcggccga gcgcttcggt
     4801 gcaccactcc ggcgtgagat cgccgacgca cgtcgggatc accggtgccc cttgcgctgc
     4861 gccttcacgc ccaacagcag cacttcggtg aggcggcggg gcgtctcgac gatcatcggg
     4921 aacaacgcgt cgcgatacgg caggcgcaca tgacgccgat cgtgctcgag cgcgtgcacg
     4981 atcgcgccga cgacggtctc gacttcgagg tcggggagca gctgcaagtg cttcatgcgc
     5041 ttgaacgcgc gcttggtcgg gtcgtgcgac tccacgttgt cgagcatcga cgtcacgacg
     5101 gtaccgatct cgacgagcgt cgtcttgatc ggcgtgccct tgcactccgc gcgtaggccg
     5161 gcagtgaagt ggctgagccc ggccttcgac gcggagtacg gcgcgagacc gggcacggcg
     5221 ttcgtggccg ccagggacga cacgttgacg atgtgacctc gtttccgggc ccgcatcccg
     5281 ggcagcaccg cgcgagacaa caggacgggc gcgacgaggt tgagcgtgaa cagctgctcg
     5341 atctggtcgg cccgcatctc ggtcaactcg ccgacgagat cgacgccggc gttgttgacg
     5401 agaaggtcga tcgggccacc ggcctcgatc cgatcgacga gatcggcgat ctcccgcgtc
     5461 ttcgtgagat cggcggggaa cgcctcgccg ccggtgttgg ccgcgagctt ctcgagcgcg
     5521 tcggcgtcgc gcgcgaccag gacgacgtgc gcgccgcggc tggccaacgc gtgggtcaac
     5581 gcttcgccga tgccgcgcga cgcgccggtg acgacgacac gagccccgtc gatcttcatg
     5641 ccctcagtcc tcccgcttcg cgcaccgtgc ctcctgcgtt cgaccgcacc ggtaagcatg
     5701 gcgcagctcg gcctacgctg acgagatgga ctcgtttcgg ggttggccgg ccgatgcatt
     5761 ggcgttctac gccgggctcg aagccgacaa ctcgcggacc tactggcagg cgcacaaggc
     5821 cacctacgac gactcggtca aggcgccgtt cctcgcgttg tccgaggtgg tggccaagga
     5881 gttcggcccc ctgcgcctct tccggccgca ccgcgacacg cgtttctcca aggacaagac
     5941 gccgtacaag accgcgcagg gcgccgtcac cgaaggtcgc ggcggcacca tgtactacgt
     6001 cgcgctctcg gccgaaggcc tgtacgccgg gagcggctac tacatgttcg gccccgacca
     6061 gctcgagcgc tggcgggcag ccgttgccga cccgcggagc ggccccgcgc tcgagcgggc
     6121 act
//
LOCUS       DASUGA010000195         2216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_173294, whole genome shotgun sequence.
ACCESSION   DASUGA010000195 DASUGA010000000
VERSION     DASUGA010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173294"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VFX21_03830"
     CDS             complement(<1..383)
                     /locus_tag="VFX21_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5095112.1"
                     /translation="MTLDSSVMNVSIATVAKDVDTTVTGIQTAITFYTLVMAALMITG
                     GKVGGILGRKRAFAIGCVIYGVGSFTTAISQSLTVLIIGWSVLEGIGAALIMPAIVAL
                     VASNFGPSERPRAYGLVAAAGAIAVA"
     gene            482..1696
                     /locus_tag="VFX21_03835"
     CDS             482..1696
                     /locus_tag="VFX21_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5095113.1"
                     /translation="MRSPEVDFDIFEASTLADSDASWRELRDAHAVAWTEHNGGHWVI
                     TRHEEVAEAFRAWEQFSSARTDPARSAIAISSNNVPLLIPEELDPPEWHPMRRILAQQ
                     LSPGASERLRPRARHWARACIDKVIESGTSNLVDDIAFPVPAAVTLEWLGFPQSDWHA
                     MSQAFHDAAAYETGTPEYKAALAAFGQVMVRVADEVAKREQEPRDDGLTALVYQEIEG
                     ERIPRDIAEALVFMTVGGGVDTTSALTSAALLHLHQHPDDRQRLLEDPELFDSATEEF
                     LRCYPPARTHARTIAEDTVFAGCPMKKGDRVLLSEASAGRDERQFPDADTFVIDRFPN
                     RHLSFGAGLHRCPGSHLARITFTETLREVLERIPDYRILDDEIVEYPNWFMIGGWAKL
                     PAVFTPGLRLGD"
     gene            1725..1916
                     /locus_tag="VFX21_03840"
     CDS             1725..1916
                     /locus_tag="VFX21_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019068096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX5095114.1"
                     /translation="MRVRVDVDKCQGHARCAALAPDVYELDELGYNVTPLTDVAPEQE
                     EAARKGALACPELAITIEE"
     gene            complement(1924..>2216)
                     /locus_tag="VFX21_03845"
     CDS             complement(1924..>2216)
                     /locus_tag="VFX21_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095115.1"
                     /translation="HAVEAVGSTDALVLVYLYQGMASWSSTLIRPQSFGPDLPWRTGP
                     VQCTDANHDVTVRTLSFTDNSARLSVMVVVGKAATPQRHAEVYAVLDSLRVE"
BASE COUNT          363 a          784 c          745 g          324 t
ORIGIN      
        1 gcgactgcga tcgcaccggc ggccgcgacc aagccgtacg cacgtggtcg ctccgacggt
       61 ccgaagttcg acgccaccaa ggcgacgatc gccggcatga tcagcgcggc accgatgccc
      121 tcgagcaccg accagccgat gatgagcacg gtgaggctct gcgagatcgc ggtggtgaac
      181 gagccgacgc cgtagatcac gcacccgatg gcgaacgcgc gcttgcggcc gaggatcccg
      241 ccgaccttgc cgcccgtgat catgagcgcg gccatgacca gtgtgtagaa ggtgatcgcg
      301 gtctggatcc cggtgacggt ggtgtcgacg tccttggcga cggtcgcgat cgacacgttc
      361 atcaccgagc tgtcgagcgt catgaggaac tgacccgccg ccagcgtcaa cagcacgatc
      421 ccggacgcct tggcgaccgc ttcggttgca ggcctctcca gcacgctggg acgataccga
      481 catgcgctca cccgaggtgg acttcgacat cttcgaggcg tcgacgctcg cggactccga
      541 cgcgagttgg cgcgagctac gcgacgccca cgccgtggcg tggaccgagc acaacggcgg
      601 ccactgggtc atcacccgtc acgaagaagt cgcggaggcg tttcgcgcct gggagcagtt
      661 ctcctccgcc cgcaccgatc cggcgcggtc ggccatcgcg atcagctcga acaacgtgcc
      721 gctcctgatc ccggaggagc tcgacccgcc ggagtggcac ccgatgcggc gcatcctcgc
      781 ccagcagctc tcgccgggcg cctcggagcg gctccgtccg cgcgcgcggc actgggcgcg
      841 tgcctgcatc gacaaggtga tcgagtcggg gacgtcgaac ctcgtcgacg acatcgcttt
      901 cccggtgccg gccgcagtga cgctcgagtg gctcggcttc ccccagtccg actggcacgc
      961 gatgtcgcag gcgttccacg acgcggcggc gtacgagacc ggcacgcccg agtacaaggc
     1021 cgcgctcgcc gcgttcggcc aagtgatggt gcgcgtggcg gacgaggtcg cgaagcggga
     1081 gcaggaaccg cgcgacgacg gcctcaccgc gctcgtgtac caggagatcg agggcgagcg
     1141 catcccgcgc gacatcgccg aggcgctcgt gttcatgacc gtcggcggcg gtgtcgacac
     1201 cacttccgcg ctcacgagcg cggcgttgct gcacttgcac cagcaccccg acgaccggca
     1261 gcggctgctc gaggacccag agctcttcga cagcgcaacc gaggagttcc tgcgctgtta
     1321 tcccccggcg cgcacgcacg cacgcacgat cgctgaagac accgtcttcg cgggctgccc
     1381 gatgaagaag ggtgaccggg tgctcttgag cgaggcgtcg gccggccgcg acgagcgtca
     1441 gttccccgac gccgacacct tcgtcatcga ccgtttcccg aaccggcacc tctcgttcgg
     1501 cgctggcctg caccggtgcc ccgggtcgca cctcgcgcgc atcacgttca ccgagacgct
     1561 gcgagaggtg ctcgagcgca tccccgacta ccgcatcctc gacgacgaga tcgtcgagta
     1621 cccgaactgg ttcatgatcg gcggctgggc gaagctgccc gccgtcttca cgcccggcct
     1681 gcggctgggc gactgagtgc gttgacgagc aggaggagac tgcgatgcgc gtgcgagtcg
     1741 acgtcgacaa gtgccagggc cacgcgcgct gtgcggcgct cgcgcccgac gtctacgagc
     1801 tcgacgagct cggctacaac gtgacgccgc tcaccgacgt cgctcccgag caggaggagg
     1861 cggcgcgcaa gggcgcgctc gcctgccccg agctcgccat caccatcgag gagtgacgct
     1921 cggtcactcg acgcggagcg agtcgagcac cgcgtacacc tcggcgtgac gttgcggtgt
     1981 tgccgccttg cccaccacga ccatcacact gaggcgcgcc gagttgtccg tgaacgagag
     2041 cgtgcgcacg gtcacgtcat ggttggcgtc ggtgcactgc accggccccg tgcgccacgg
     2101 caggtcgggt ccgaacgact gcggtcggat cagtgtcgac gaccagctcg ccatgccctg
     2161 gtacaggtag acgagcacga gcgcgtcggt cgaaccgacg gcttccaccg catgct
//
LOCUS       DASUGA010000196         2287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_451157, whole genome shotgun sequence.
ACCESSION   DASUGA010000196 DASUGA010000000
VERSION     DASUGA010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451157"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VFX21_03850"
     CDS             complement(<1..187)
                     /locus_tag="VFX21_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX5095116.1"
                     /translation="MATEADVRRIALSLPETTEKPSYGTPGFRVKDKLFLRIRSNDEG
                     GLVVFVENLGEKEALLAS"
     gene            complement(200..1054)
                     /locus_tag="VFX21_03855"
     CDS             complement(200..1054)
                     /locus_tag="VFX21_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX5095117.1"
                     /translation="MFRNSLKTAALLALIGALCLGVGSLFGTGGLVIGLGIGLVFVGG
                     SYWFSDKLAVKAAGAQPVTEAQAPQLYAIVRDLTTRADMPMPAIYISPAAQPNAFATG
                     RNPHHAAVAVTQGLLQVVDEDELRGVLAHELSHVQNRDILISSVAAAVALGITFIARM
                     AMWGAIFGGGQGNDRDGGNVFGALAMMILAPIAATMLQLALSRSREFEADRSGAELLG
                     TGEPLARALQKIEGYAKQVPMNIDPAHASAFIVNPLTGRKMQFSNLFMTHPPTEERVR
                     RLQHQHQH"
     gene            complement(1095..2189)
                     /locus_tag="VFX21_03860"
     CDS             complement(1095..2189)
                     /locus_tag="VFX21_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX5095118.1"
                     /translation="MAISAISLAPIDRFLNTLWERGGSDLLLTAYNRPLVRIDGHLLP
                     IEEEPVLDEDHVAHLVLSTLTEELKQDLRINKEVDFSFAWNGVARFRANCFFQQGALA
                     MALRIIPLKLPTFNELGLPPAVEYFSNLPQGLVLVTGPTGSGKSTTLAAMINYINENR
                     AAHLLTIEDPIEYVHPHKRCAVSQREVGYDTLSFERALRAALREDPDVILVGEMRDPE
                     TVQFALSIAETGHLVFATLHTNDAPQALDRISDMFPAERQNQIRVQLAACISGVVSQR
                     LVPRIGGGMAAAFEILIANNAVRSLVREGKTHQIRNIISSGHKEGMCTLESWMNHLVA
                     NGIVTYEDAFARSMHPREIKPPVGQTAYSG"
BASE COUNT          365 a          740 c          795 g          387 t
ORIGIN      
        1 cggacgcgag cagcgcttcc ttctcgccga ggttctccac gaacacgacc aagccgcctt
       61 cgtcgttcga gcggatgcgc aggaacagct tgtccttcac ccggaacccg ggtgtgccgt
      121 acgacggctt ctcggtcgtc tccggcagtg acagcgcgat ccggcggacg tcggcttcgg
      181 tggccatgcc accacggtgt cagtgctggt gctggtgctg caagcggcgc acgcgctctt
      241 cggtcggcgg gtgcgtcatg aagaggttcg agaactgcat cttgcggccc gtcagcgggt
      301 tcacgatgaa cgcggaagcg tgtgccggat cgatgttcat cggcacctgc ttcgcatagc
      361 cctcgatctt ctggagcgca cgagccagcg gctctccggt accgagcaac tcggcaccgg
      421 agcggtcggc ttcgaattcc cgtgaccgcg acagtgcgag ttggagcatg gtcgccgcga
      481 tcggcgcgag gatcatcatc gccagcgcac cgaacacgtt gccaccgtcg cggtcgttgc
      541 cctgaccgcc accgaagatg gcgccccaca tcgccatgcg ggcgatgaac gtgatgccga
      601 gcgccactgc cgccgccacc gacgagatca ggatgtcgcg gttctggacg tgcgacagct
      661 cgtgggcgag cacaccgcgc agctcgtcct cgtcgacgac ctgcagcaag ccctgcgtca
      721 cggcgaccgc cgcgtggtgc gggttgcggc ccgtcgcgaa cgcgttgggc tgcgcggcgg
      781 ggctgatgta gatggcaggc atgggcatgt cggcgcgcgt cgtgaggtcg cgcacgatcg
      841 cgtagagctg cggggcctgc gcttcggtca ccggctgcgc accggcggcc ttcaccgcga
      901 gcttgtcgga gaaccaatac gacccgccga cgaacaccag gccgatgccg agcccgatga
      961 cgagcccgcc cgtgccgaac aaggaaccga caccgaggca cagcgcgccg atgagagcga
     1021 ggagcgccgc ggtcttcaac gagttgcgga acatgctccc tacaacgtag ggcctggccc
     1081 gtccgttccc agcgtcagcc cgaataggcg gtttgtccga ccggcggctt gatctcccgg
     1141 gggtgcatcg accgggcgaa ggcgtcctcg taggtgacga tgccgttggc gacgaggtga
     1201 ttcatccagc tctcgagcgt gcacatgccc tctttgtggc ccgagctgat gatgttgcgg
     1261 atctggtgag tcttgccctc ccgcaccagc gaccggaccg cgttgttcgc gatgaggatc
     1321 tcgaaggccg ccgccatgcc gccgccgatg cggggcacga gccgctggga cacgacaccg
     1381 gagatgcacg ccgcgagctg cacgcggatc tggttctgcc gttcggccgg gaacatgtcg
     1441 gagatgcggt cgagcgcctg gggcgcgtcg ttggtgtgca gcgtcgcgaa cacgaggtgg
     1501 ccggtctcgg cgatcgacag cgcgaactgc accgtctcgg ggtcgcgcat ctcgccgacg
     1561 aggatgacgt cggggtcttc ccgcagcgcg gcgcgcagtg cccgctcgaa gctgagcgtg
     1621 tcgtagccga cctcgcgctg gctgaccgcg caacgcttgt gcgggtgcac gtactcgatc
     1681 gggtcttcga tcgtgagcaa gtgcgccgcg cggttctcgt tgatgtagtt gatcatcgcg
     1741 gcgagcgtcg tgctcttgcc ggaaccggtc gggccggtca ccagcacgag cccctgcggc
     1801 aggttcgaga agtactcgac ggccggcggc agaccgagct cgttgaaggt cggcagcttc
     1861 agcggaatga tccgcagcgc catcgcgagc gcgccctgct ggaagaagca gttggcgcgg
     1921 aagcgggcaa caccgttcca cgcgaacgag aagtcgactt ccttgttgat gcgcaggtct
     1981 tgcttcaact cttcggtgag ggtgctgagc accaagtgcg cgacgtggtc ctcgtcgagc
     2041 accggctcct cctcgatggg gagcaggtga ccgtcgatgc gcacgagcgg ccggttgtac
     2101 gccgtcagga gcaggtcgga accgccgcgc tcccagagcg tgttcaagaa ccggtcgatg
     2161 ggcgcgaggc tgatcgcgct gatggccatg agccgtaccc tccgcagctg ccgtcgaatt
     2221 tggtttcggc gcgctcgtac gcggcattga gggtcattcc gccgagcgca cgagaagggg
     2281 ccccgcc
//
LOCUS       DASUGA010000197         6279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1719145, whole genome shotgun sequence.
ACCESSION   DASUGA010000197 DASUGA010000000
VERSION     DASUGA010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6279
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719145"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VFX21_03865"
     CDS             <1..250
                     /locus_tag="VFX21_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX5095119.1"
                     /translation="DLQAEMGFPGLHVVQQGEVQSARAFFPEEGDYTFYCAIPGHRAA
                     GMEGSIAVTGDPKTLDEAKAEVGDTGEAGAAGTETPTS"
     gene            complement(247..1122)
                     /locus_tag="VFX21_03870"
     CDS             complement(247..1122)
                     /locus_tag="VFX21_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX5095120.1"
                     /translation="MPWTVIVNPAAGRGRTAKLLDDLTAAARAAGATVEVSPNPDAPA
                     RLAKEAASAGHDLVAAGGDGVVSEVAGIAADTGRRLAIVPTGAGNDFARELGYDTKKP
                     LSALDALADGTDRTVDLGRCNGRWYTCVTASGFDAEANRWANTVQRLSGTTLYVAAVL
                     RTLAVYKPHPFRLTVDGEAHEMRAWLVAVGNGPAYAGGMHITPDARMDDGLLDVTVIG
                     DLSRARFLVNFPKVFKGTHVSHPRVSTFRAEHVELASLDDRVPMEVYADGERVGPLPA
                     TMAAVRNALTVRVPS"
     gene            1167..1886
                     /gene="uppS"
                     /locus_tag="VFX21_03875"
     CDS             1167..1886
                     /gene="uppS"
                     /locus_tag="VFX21_03875"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016691344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX5095121.1"
                     /translation="MVHLDPDRMPKHVALVMDGNGRWAQRRGLKRTDGHAAGEEALFD
                     TVEGGLDIGLAWMTVFAFSTENWRRPLDEVRFLMNFNERLLVDRREELYERGVRVLFL
                     GRRGGRVPARVRRRIEETEELTRKNKEMTLAFAFNYGSRAEIVDATREIGRLVKAGKL
                     DPEKIDERTIERHLYAPDMPDPDLLIRTSGEFRISNYLLWQLAYSELVFTDVLWPDFR
                     RENLYEAIAEFQKRNRRFGAV"
     gene            1974..2699
                     /gene="recO"
                     /locus_tag="VFX21_03880"
     CDS             1974..2699
                     /gene="recO"
                     /locus_tag="VFX21_03880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX5095122.1"
                     /translation="MQSHGLYRDEGVVLRTIKLGEADRIITFFTQGHGKVRAVAKGIR
                     KTTSKFGARLEPASRVQVQCYRGRELDVVTQAETVEANRELREEYVLLTHTVPILEAV
                     DQVAQEREANPALYRMLTGALRTLGERRTPLVTPAFFWKLLSLEGFHPMLDGCARCAE
                     GDRELTAFDLEEGGTICRSCAHGAGRPITPAALELLRRMLGGGLNGALTETVGPTTYE
                     VEQLGIRVLEHHVERRLRSTTLL"
     gene            2787..4097
                     /locus_tag="VFX21_03885"
     CDS             2787..4097
                     /locus_tag="VFX21_03885"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX5095123.1"
                     /translation="MDRVVNLAKRRGLVFPSSEIYGGFRSTWDYGPLGVLLKRNVKDA
                     WWRAMVQERDDMVGLDAAILMAPKVWEASGHVATFSDPLVDCRNCNERFRADHLPESG
                     ACPNCGAKESFTEARQFNLMFKTYVGPVENDASVAYLRPETAQGIFVNFKNAQTTSRK
                     KPPFGIAQIGKSFRNEITPGNFVYRTREFEQMEMEYFVPPEQGAQWFEYWVQQRFNWY
                     VDLGIPEDKLRIRPHDPDELSHYSAGTSDIEFMFPWGWGELEGIANRTDYDLTQHAKF
                     GGVDLTYFDQERNEHYVPHVIEPAAGADRATLAFLLAAYDEDEAPTGEGKVEKRTVLR
                     LDRRLAPIKVAVLPLSKNEKLVPLADEVAKSLRKHFQIDVDLAGAIGRRYRRQDEVGT
                     PLCVTIDFDSLDDGAVTVRDRDTMAQDRVPIDGLTDELRARLEV"
     gene            4106..5128
                     /locus_tag="VFX21_03890"
     CDS             4106..5128
                     /locus_tag="VFX21_03890"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX5095124.1"
                     /translation="MSDDYYDLLGIDADAPTEEIRTAYREKKDALNEKGDKSGVAQLN
                     KAWNVLSDPYQRGRYDAERENGNNVSDALDGDDITVEDAPPPRRRRFLEPPDRSNQPA
                     RPVPVAPAGMTFPPPRRRIYAMVIDLFIVFGIFLGGLALSQAVLNANHKADVDRQKDL
                     SDQITDARDEFNDLDDAADKAEDRHDNLVDANASQADIDEAEAAAREARAKADAQQRE
                     VDALQDQYDDVTAKLSPTSTAFTVGAFVLGLLYLVVPSAFGGQTLGKKIMRVRVVRED
                     GSPINFWAAMFRYGIIAFPTFLAGATLLGPLAAAIALIVVLGWMRNAKLQGLHDRTAK
                     TLVVEA"
     gene            5163..>6279
                     /locus_tag="VFX21_03895"
     CDS             5163..>6279
                     /locus_tag="VFX21_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX5095125.1"
                     /translation="MTKYVYAFEEGSKEQKFLLGGKGANLAEMTNLGLPVPPGFTIST
                     DACKAYMAGGDAMPDGLMIEVAAARTALEAKMDKTLGDANDPLLVSVRSGAAFSMPGM
                     MDTVLNLGLNDESVKGLAKQTNNERFAYDSYRRFVQMFGKIVLDIPGDLFEEALHDLV
                     EEKGLNVETELRAADLASLVETFKGIVKKEAGVDFPTDPTEQLAYAIEAVFKSWNGRR
                     AKDYRKLEGISDDLGTAVNVQTMVFGNKGDDSGTGVAFTRNPSSGENTPYGDFLTNAQ
                     GEDVVAGIRITEPLDAMGNDFPECHKQLLDVMSLLENHYRDMCDIEFTIEQGRLFMLQ
                     TRVGKRTAAAALRMAVEMEQEGLIDAREAVLRVQPAQL"
BASE COUNT         1105 a         2139 c         2124 g          911 t
ORIGIN      
        1 cgatctgcag gccgagatgg gcttccccgg actgcacgtc gtgcaacagg gcgaagtgca
       61 gtcggcgcgg gccttcttcc ccgaagaggg cgactacacc ttctactgcg ccatcccggg
      121 ccaccgcgcc gcgggcatgg agggttcgat cgcggtgacc ggcgacccga agacgctcga
      181 cgaggccaag gccgaggtcg gcgacacggg tgaggccggt gccgcgggca ccgagacgcc
      241 gacttcctag ctcggcacgc gcaccgtgag cgcgttgcgg acggccgcca tcgtggccgg
      301 cagcggtccg acgcgctcac cgtcggcgta gacctccatc ggcacgcgat cgtcgagcga
      361 tgcgagctcg acgtgctcgg cgcggaaggt cgacacccgc gggtgcgaca cgtgcgtgcc
      421 cttgaagacc ttggggaagt tcacgaggaa cctcgcgcgc gagaggtcgc cgatcacggt
      481 gacgtcgagc agcccgtcgt ccatgcgggc gtcgggtgtg atgtgcatgc cgcccgcgta
      541 cgcggggccg ttgccgaccg cgacgagcca ggcgcgcatc tcgtgcgctt cgccgtcgac
      601 ggtgaggcgg aacggatgcg gcttgtagac cgcgagcgtg cgcaacacgg ccgcgacgta
      661 cagcgtcgtg ccggagagcc gttgcaccgt gttggcccac cggttcgctt ccgcgtcgaa
      721 acccgacgcg gtgacgcacg tgtaccaccg gccgttgcag cgcccgaggt cgacggtgcg
      781 gtcggtgccg tcggcgagcg cgtcgagtgc gctgagcggc ttcttcgtgt cgtagccgag
      841 ctcgcgcgcg aagtcgttgc cggcgccggt cggcacgatc gcgagccgcc gtccggtgtc
      901 ggccgcgatc ccggccacct ccgacacgac gccgtcgcca ccggctgcga cgaggtcgtg
      961 gcccgcgctt gcagcttcct tggccagacg cgcgggcgcg tcggggttgg gcgacacttc
     1021 gacggtcgcg ccggcggcgc gcgcggcggc ggtcaggtcg tcgagcagct ttgcggtgcg
     1081 acctcgcccc gcggcggggt tgacgatcac ggtccacggc acggctcagg acgttatcga
     1141 gccgcagcta ctactttcag gggctgatgg tgcacctcga tccggaccgg atgccgaagc
     1201 acgtggcgct ggtcatggac ggcaacggcc ggtgggcgca gcgccggggc ctcaagcgca
     1261 ccgacgggca cgcggcgggt gaggaggccc tcttcgacac cgtcgaaggc gggctcgaca
     1321 tcggcctggc gtggatgacc gtgttcgcgt tctcgaccga gaactggcgc cgcccgctcg
     1381 acgaagtgcg cttcctcatg aacttcaacg aacggttgct cgtcgaccgc cgggaggaac
     1441 tgtacgaaag gggagtgcgc gtgctgttcc tcggccggcg cggcgggcgg gtgcccgcgc
     1501 gcgtgcgccg gcgcatcgag gaaaccgagg agctcacgcg taagaacaag gagatgacgc
     1561 tcgcgttcgc gttcaactac ggctcacgcg cggagatcgt cgacgcgaca cgcgagatcg
     1621 gtcggttggt gaaggcgggc aagctcgacc ccgagaagat cgacgagcgc acgatcgaac
     1681 gtcatctgta cgcgcccgac atgcccgacc ccgacctgtt gatccgcacg tcgggcgagt
     1741 tccgcatctc caactacctg ctgtggcagc tcgcgtactc ggagctggtg ttcaccgacg
     1801 tcctctggcc cgacttccgc cgcgagaacc tctacgaagc catcgccgag ttccaaaagc
     1861 gcaaccgccg cttcggcgcc gtctgaaccg gccaccgcgc gtcgggcgtc tgagaagggc
     1921 ggaattcgcc cttgtaagac gcccgaccaa cgccgaatta cactgctgta agcatgcaga
     1981 gccacgggct gtaccgggac gagggggtcg tgctgcgcac gatcaagctc ggtgaggccg
     2041 accgcatcat cacgttcttc acccagggcc acggcaaggt gcgcgccgtc gccaagggga
     2101 tccgcaagac gacgtcgaag ttcggggcgc ggctcgagcc cgcgagccgg gtccaggtgc
     2161 agtgctaccg ggggcgcgag ctcgacgtcg tgacgcaggc cgagacggtc gaggcgaacc
     2221 gcgagctgcg cgaggaatat gtgctcctca cgcacacggt gccgatcctc gaagcggtcg
     2281 accaggtcgc gcaggagcgc gaggccaacc ccgcgctgta ccggatgctc accggcgcgc
     2341 tgcgcacact cggtgaacgg cgcaccccgt tggtgacgcc cgcgttcttc tggaagctgc
     2401 tctcgctcga aggcttccac ccgatgctcg acggttgcgc gcgctgcgcg gaaggcgacc
     2461 gggagctgac cgcgttcgac ctcgaggagg gcggcacgat ctgccggtcg tgtgcgcacg
     2521 gcgccggccg cccgatcacg cccgccgcgc tcgaactgct ccggcgcatg ctcggcggcg
     2581 gcctgaacgg cgcgctgacc gaaacggtcg ggccgacgac gtacgaggtg gaacagctcg
     2641 ggatccgggt gctcgagcac cacgtcgagc gccgcctccg ctccacgacc ctgctctgag
     2701 cgccgccgac cggggtgctt cgggccgggg agaaaccggt ccggccggta gcctggggag
     2761 tcatggccgg gcccgatccg aaactgatgg accgcgtcgt caatctcgcc aaacgccggg
     2821 gcctggtgtt cccgtcgtcg gagatctacg gcgggttccg ctccacctgg gactacgggc
     2881 cccttggcgt cctcctcaag cgcaacgtga aggacgcttg gtggcgagcg atggtccaag
     2941 agcgcgacga catggtcggc ctcgacgcgg ccatcctcat ggcccccaag gtctgggagg
     3001 ccagcgggca cgtcgccacc ttctccgacc cgctggtcga ctgccgcaac tgcaacgagc
     3061 gcttccgcgc cgaccacctc cccgaatcgg gtgcgtgccc gaactgcggc gccaaggaga
     3121 gcttcaccga ggcccgccag ttcaacttga tgttcaagac gtacgtcggc ccggtggaga
     3181 acgacgcgtc ggtcgcgtac ctgcggcccg agaccgcgca gggcatcttc gtcaacttca
     3241 agaacgcgca gacgacgtca cgcaagaagc cgccattcgg catcgcgcag atcggcaagt
     3301 cgttccgcaa cgagatcaca cccggaaact tcgtctaccg cacgcgcgag ttcgagcaga
     3361 tggagatgga gtacttcgtg ccgcccgagc aaggcgcgca gtggttcgag tactgggtac
     3421 agcagcggtt caactggtac gtcgacttgg gcatccccga agacaagctg cgtatccgcc
     3481 cgcacgaccc cgacgagttg tcgcactaca gcgcgggcac gtcggatatc gagttcatgt
     3541 tcccgtgggg ctggggcgag ctcgaaggca tcgcgaaccg caccgactac gacctcacgc
     3601 agcatgcgaa gttcggaggc gttgacctca cctacttcga ccaggagcgc aacgagcact
     3661 acgtgccgca cgtgatcgag cccgcggccg gcgccgaccg ggccacgctc gcattcctgc
     3721 tcgcggccta cgacgaggac gaagcaccga cgggcgaggg caaggtcgag aagcgcacgg
     3781 tgctgcgact cgaccggcgc ctggccccga tcaaggtcgc ggtgctcccg ctgtcgaaga
     3841 acgagaagct cgtcccgctc gccgacgagg tggcgaagtc gctgcggaag cacttccaga
     3901 tcgacgtcga cctggccggc gccatcggcc gccgttaccg gcgccaggac gaagtaggca
     3961 cgccgctgtg cgtgacgatc gacttcgatt cactcgacga cggagccgtc accgtgcgcg
     4021 accgggacac gatggcccaa gatcgggtcc cgatcgacgg cctgaccgac gaactgcgag
     4081 cgagactcga ggtttgagca aagcaatgag cgacgattac tacgacctgc tcggcatcga
     4141 cgccgacgcg ccgaccgaag agatccgcac cgcgtaccgc gagaagaagg acgcgctgaa
     4201 cgagaagggc gacaagagcg gcgtcgcgca gctgaacaag gcgtggaacg tcctctccga
     4261 cccgtaccag cgcggccgct acgacgcgga gcgcgagaac ggcaacaacg tctcggacgc
     4321 gctcgacggc gacgacatca cggtcgaaga cgcgccgccg ccgcggcggc gccggttcct
     4381 cgaaccgccc gaccgctcca accagccggc gcgtccggtg ccggtcgcgc ccgcgggcat
     4441 gacgttcccg ccgccgcgcc gccgcatcta cgcgatggtc atcgacctgt tcatcgtgtt
     4501 cggcatcttc ctcggcggct tggcgctgtc gcaagcggtg ttgaacgcga accacaaggc
     4561 cgatgtcgac cggcagaagg atctctccga ccagatcacc gacgcgcgcg acgagttcaa
     4621 cgacctcgac gacgcggccg acaaagccga ggaccgtcac gacaacttgg tcgacgccaa
     4681 cgcgtcgcaa gccgacatcg acgaggccga ggctgcggca agggaggccc gggccaaggc
     4741 cgacgcgcag caacgcgagg tcgacgcgct ccaggaccag tacgacgacg tcaccgcaaa
     4801 gctgtcgccc acgagcacgg ccttcaccgt cggtgcgttc gtgctcggct tgttgtatct
     4861 cgtcgtgccg agcgcgttcg gtggccagac gctcggcaag aagatcatgc gcgtccgggt
     4921 cgtccgcgag gacggctcgc ccatcaactt ctgggctgcg atgttccgct acgggatcat
     4981 cgcgttcccg acgttcctgg ccggcgcaac gctgttgggc ccgctcgcgg ccgcgatcgc
     5041 gttgatcgtc gtgctcggct ggatgcgcaa cgccaaactg cagggtctgc acgaccgcac
     5101 cgccaagacg cttgtcgtcg aagcgtgacc cgttcgaaca actgtgtgaa agaaggctcc
     5161 cgatgacgaa gtacgtctac gcgttcgagg aggggagcaa ggaacagaag ttcctcctcg
     5221 gcggcaaggg cgcgaacctc gccgagatga ccaacctcgg tctgcccgtc ccgcccggct
     5281 tcacgattag caccgacgcc tgcaaggcct acatggcggg cggcgacgcg atgcccgacg
     5341 gtctgatgat cgaggtggcc gccgcgcgca ccgcgctcga agccaagatg gacaagacgt
     5401 tgggcgacgc caacgacccg ctgctcgtct ccgtgcgttc cggtgctgcg ttctcgatgc
     5461 ccggcatgat ggacaccgtc ctcaacctcg ggttgaacga cgagtcggtg aagggcctcg
     5521 ccaagcagac gaacaacgag cgcttcgcgt acgactcgta ccgccgcttc gtgcagatgt
     5581 tcggcaagat cgtgctcgac atccccggcg acctcttcga ggaagcactg cacgacctcg
     5641 tcgaggagaa gggcctcaac gtcgagaccg agctgcgcgc ggccgacctc gcgtcactcg
     5701 tcgagacgtt caaggggatc gtgaagaaag aagccggcgt cgacttcccg accgacccga
     5761 ccgaacagct cgcctacgca atcgaagcgg tcttcaagtc ctggaacggc cgccgcgcca
     5821 aggactaccg caagctcgaa ggcatctccg acgacctcgg taccgcggtc aacgtgcaga
     5881 cgatggtgtt cggcaacaag ggcgacgact ccggcaccgg tgtcgcgttc acgcgcaacc
     5941 cgtcgtcggg tgagaacacg ccctacggcg acttcctcac caacgcgcaa ggtgaagacg
     6001 tcgtcgccgg catccgcatc accgagccgc tcgacgcgat ggggaacgac ttcccggagt
     6061 gtcacaagca gctgctcgac gtcatgtcgc tcctcgagaa ccactaccgc gacatgtgcg
     6121 acatcgagtt cacgatcgag cagggtcgcc tcttcatgct gcagacgcgt gtcggcaagc
     6181 gcaccgccgc cgcggccctg cgcatggccg tcgagatgga gcaggagggc ctgatcgacg
     6241 cgcgcgaggc ggtgctgcgg gtccagcccg cgcagctcg
//
LOCUS       DASUGA010000198         3882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_727977, whole genome shotgun sequence.
ACCESSION   DASUGA010000198 DASUGA010000000
VERSION     DASUGA010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727977"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VFX21_03900"
     CDS             complement(<1..612)
                     /locus_tag="VFX21_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095126.1"
                     /translation="MTTTTPATETHARGRAGLVIALVIGLVIVPVAVAFVALWGADWK
                     PASDIAVEVLRVRDVGGRHTPFTGVQSRFGWDHPGPMLFWYLAPWYRLFGTTGVMYAV
                     GALNIAALVGALVVARRRGGVLLMALVGIVALLLVRSLTLDLLVDPWNPWVAVLPFLL
                     FVLLAWSVAERDRGALVWLVAVGTFLVQTHVGYALLFVGAGAAA"
     gene            668..2020
                     /locus_tag="VFX21_03905"
     CDS             668..2020
                     /locus_tag="VFX21_03905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5095127.1"
                     /translation="MTAARRERSSFAVGLLLIALAALTIRVLFVVVVDPEVPRIGDAS
                     AYHLLAENLAGGNGYIRPFDKELLGETRATAEYPPLFPSVVAVPAFVGVHSVEGQRLF
                     VAFIGAGTVVLIGLLGRRLGGDSCGLLAGGIAACYPMLFLGEATLMAESLSVPLATGV
                     ILLAYRLIDRPSIARAVALGAGLGLLTLARAEGLLLGVVLLIGLGWRLRAVALRQRLF
                     LATAALGVMLCLVAPWTVRNAIRLHAFVPVSNNVATLVDGANCDWVYSGHHVGLWRET
                     FSTFGADARTRSQAEACFEGFAIEDPGFDEAEVASNHRRDGTDYAQHHIGSLPKVAVA
                     RVLRTWALFAPRQQVNFESLEGRPRDWQMAGTVMFWVLLPFAIGGLVLLARRRIPVWP
                     LHATVITVTVVAALTYGQQRFRINAEPVIVACAALAIVALVMRLRARYTRSDPPHRPG
                     "
     gene            2032..2355
                     /locus_tag="VFX21_03910"
     CDS             2032..2355
                     /locus_tag="VFX21_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095128.1"
                     /translation="MLSFERSVVGALMDPAADAERRRAITDYVDTNLRSMPEHLRAGV
                     FAESLVLQTWVRVSARPGTADERRLRARVERWQYSRIDLVRQYVRLLQSLVLFAENEL
                     EPAAA"
     gene            2352..3773
                     /locus_tag="VFX21_03915"
     CDS             2352..3773
                     /locus_tag="VFX21_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX5095129.1"
                     /translation="MTTRDTDVLIIGSGAGGAVTAASLARAGRDVLVVEEGPWVDPDA
                     LEPFSLEEMVAKYRHCGSSAALGRPAIAYAEGRCVGGSTEINSGLYHRLPDDLAREWT
                     QTYEIDEFGPDVLARYSDRVENELSVSRLPGAPPASSAVLERGASKLGWRAVEFSRVF
                     RYEHGGRAVKQTMARTYLPRAIDDGAEILANTRVVKLVRGKDRIEGARCRRTRPDGSV
                     EDLFIRADHVFVCGGAIHTPALLQRSGIWRNIGNGLKMHATVKIAARFPHPIDHGDVP
                     MHRVTEFAPSLTIGGSASRRGHVALALADSAADYADALANWEHVAVYYAAIRSEGSGR
                     VIALPRVSSPLVTYNLTDADMSRLARSLVHLGEVLLAAGATELYPSATGAVVARNTND
                     LGKWWDAVTRNRANLMTVHLTSTVRIGEHRARTGSDSFGRVWGYENLRVNDASLLPDA
                     PGVNPQGTIMALATRNSDHFLAH"
BASE COUNT          578 a         1396 c         1319 g          589 t
ORIGIN      
        1 ggcggccgcg cccgcgccga cgaagagcag cgcgtagccg acgtgcgtct gcacgaggaa
       61 ggtgccgacg gcaacgagcc agacgagtgc gccgcggtcg cgttcggcca cggaccatgc
      121 gaggagcacg aagagcagga acggcagcac cgcgacccag gggttccacg gatccaccag
      181 gaggtcgagg gtcagcgacc ggacgagcag gagcgccacg atgccgacga gcgccatcag
      241 gagtacgccc ccgcggcggc gggcaacgac cagcgcaccg acgagcgcgg cgatgttcaa
      301 cgcacccacc gcgtacatga cgccggtcgt gccgaacagg cggtaccaag gcgcgaggta
      361 ccagaacagc atggggcccg ggtggtccca accgaagcgt gactgtaccc cggtgaacgg
      421 cgtgtgccga ccgccgacgt cacgcacgcg caacacctcg accgcaatgt cggatgcggg
      481 tttccagtcg gcgccccaca gcgcgacgaa ggcgacggcg accggcacga tgacgagccc
      541 gatgacgagt gcgatgacga gtccggcgcg gccgcgcgcg tgtgtctcgg tcgcaggtgt
      601 cgtcgtcgtc atgggccggg cgagatgcta gggattggcg ggagttccgc aagtaccatc
      661 gcccgccatg accgcagccc ggcgcgaacg ctcgtcgttc gccgtcggcc tgcttctgat
      721 tgcgctggcc gcgctcacga tccgagtgct gttcgtcgtc gtcgtcgatc ccgaggtgcc
      781 gcgcatcggc gacgcgtcgg cctaccacct gctggccgag aacctcgccg gcggcaacgg
      841 ctacatccgc ccgttcgaca aggaattgct cggcgagacc cgcgcgactg ccgagtatcc
      901 gccgctgttc ccgagcgtcg tcgcggtgcc cgcattcgtc ggcgtgcaca gcgtcgaggg
      961 acagcgcttg ttcgtcgcgt tcatcggcgc gggaaccgtc gtgctcatcg gactcctcgg
     1021 ccgccgcctc ggtggcgaca gctgtggcct cctcgcgggc ggcatcgcgg cgtgctatcc
     1081 gatgctgttc ctcggcgagg ccacgctcat ggccgagtcg ttgtcggtac ccctcgcgac
     1141 cggggtgatc ctgctcgcat accggctgat cgacagaccc tcgatcgcgc gcgccgttgc
     1201 gctcggtgcg gggctcggac tgctgacgct cgcgcgcgcc gaaggcctgc tgctcggcgt
     1261 cgtgctcctg atcggcctcg ggtggcgact ccgcgcagtg gcactccggc aacgactgtt
     1321 cctcgccacg gccgcgctcg gcgtcatgct ctgcctcgtc gcgccatgga cggtgcgcaa
     1381 cgcgatccgg ctgcacgcgt tcgtgcccgt ctccaacaac gtggcgacac tggtcgacgg
     1441 cgcgaactgc gactgggtct acagcggcca ccacgtcggg ctgtggcgcg agacgttctc
     1501 gaccttcggc gccgacgccc gtacgcggtc gcaggcggaa gcatgcttcg aagggttcgc
     1561 gatcgaagat ccaggcttcg acgaagccga ggtcgcgtcg aaccatcgcc gggacggcac
     1621 cgactacgcg cagcatcaca tcggctcgct cccgaaggtc gccgtcgcgc gcgtgctgcg
     1681 cacgtgggcg ctgttcgcgc cgcgccagca ggtgaacttc gagtccctcg aagggcgacc
     1741 gcgggattgg cagatggccg gcacggtgat gttctgggtg ttgctgccgt ttgcgatcgg
     1801 cgggctcgtg ctcctcgccc ggcggcgcat cccggtctgg cctctccatg ccaccgtgat
     1861 caccgtcacg gtcgtcgccg cgctcaccta cggccaacaa cgcttccgca tcaacgccga
     1921 accggtgatc gtcgcgtgcg cggccctggc catcgtcgcg ctcgtcatgc ggctgcgggc
     1981 ccgatacaca agatcggatc cgccgcatag gcccggatag gctgcccttc cgtgctttcg
     2041 ttcgagcgca gcgtcgtcgg cgccctcatg gatcccgccg ccgacgccga gcggcgccgg
     2101 gcgatcaccg actacgtcga caccaatctc cgctcgatgc ccgagcacct gcgcgccggc
     2161 gtcttcgccg aatcactcgt gttgcagacg tgggtacgcg tcagcgctcg gcccggcaca
     2221 gccgacgaac gtcgtttgcg cgcgcgcgtc gaacgctggc agtacagccg catcgacctc
     2281 gtgcgccaat acgtgcgcct cctgcagtcg ctcgtgctgt tcgccgagaa cgagttggag
     2341 cccgcggccg catgacgact cgcgacaccg atgtcctgat catcggttcg ggcgcgggcg
     2401 gcgcggtcac cgcggcatcg ctcgcccgcg ccgggcgcga cgtgctcgtc gtcgaagagg
     2461 gcccgtgggt cgaccccgac gcgctcgagc cgttctcgct cgaggagatg gttgcgaagt
     2521 atcgccactg cggctcgtcc gcggcgctgg gccggcccgc gatcgcatac gccgaggggc
     2581 gctgcgtcgg cgggagcacc gagatcaaca gcggcctcta ccaccggctc cccgacgatc
     2641 tcgctcggga gtggacgcag acctacgaga tcgacgagtt cggtcccgac gtgctcgctc
     2701 ggtactcgga tcgcgtcgag aacgagctct cggtgtcgcg gctgccgggc gcgccgccgg
     2761 cgtcgtcggc cgtcctcgaa cgcggcgcgt cgaagctcgg ctggcgcgcg gtcgagttct
     2821 cgcgcgtgtt ccgctacgag cacggcggcc gcgcggtcaa gcagacgatg gcccgcacct
     2881 acctgccgcg cgcgatcgac gacggcgccg agatcctcgc caacacccgc gtcgtgaagc
     2941 tggtgcgcgg caaggatcgc atcgagggcg cgcgctgccg gcgtacccgc cccgacggtt
     3001 ccgtcgaaga tctcttcatc cgcgccgacc acgtcttcgt gtgcggcggt gcgatccaca
     3061 cccccgcgct gctgcagcgc agcggcatct ggcggaacat cgggaacggc ttgaagatgc
     3121 acgcgacggt caagatcgcg gcgcgcttcc cgcacccgat cgaccacggc gacgtgccga
     3181 tgcaccgcgt caccgagttc gcgccgagcc tgacgatcgg cggttccgcg agccgccgcg
     3241 gccacgtcgc cctcgcactc gccgactccg cggccgacta cgccgatgcc ctcgcgaact
     3301 gggagcacgt ggcggtgtat tacgccgcga tccgcagcga gggctcgggt cgcgtcatcg
     3361 cgcttccccg cgtcagctcg ccgctcgtca cctacaacct gaccgatgcc gacatgagcc
     3421 gcctcgcgcg ttcgctcgtc cacctcggcg aagtgttgct cgcggcgggc gcgaccgagc
     3481 tgtatccgtc ggccacgggc gcggtcgtcg cccgcaacac caacgatctc ggcaagtggt
     3541 gggacgcggt gacgcgcaac cgcgccaacc tcatgaccgt ccacctcacg tcgacggtga
     3601 ggataggcga gcaccgggcc cgaaccggct ccgacagctt cggccgggta tggggctacg
     3661 agaacctgcg cgtcaacgac gcgtcactgc tgccggacgc gccgggcgtg aacccgcagg
     3721 gcacgatcat ggctctcgcc acccgcaaca gcgaccactt cctcgcccac tgatcaacgg
     3781 gcgtcttaca acggcggatt ccgcccttgt aaggcgcccg agaatcgttt cgccgcggtg
     3841 tcgagggcga tggcggcgta gacgatcatc gagggttcgg ct
//
LOCUS       DASUGA010000199         2584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_189729, whole genome shotgun sequence.
ACCESSION   DASUGA010000199 DASUGA010000000
VERSION     DASUGA010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189729"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..962)
                     /locus_tag="VFX21_03920"
     CDS             complement(159..962)
                     /locus_tag="VFX21_03920"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5095130.1"
                     /translation="MSRAKRTRKKENARVAREARAAAAKREKRRTAVIRFGSAFLVLI
                     LIIAFISFITRDDDNSSASDSTTTTTPTTVASDRPTFDAPPPMAIDADANDYSATIST
                     SEGDIEMSLDGVNAPTSVNNFVFLARNGFYDGLMFNRAAKGFVIQTGSPNNTTAGGPG
                     YSVQAELPPNGAGYVMGSVAWAKAGNEAAGTAGSQFFIVTGDGTSLTPDYGYLGIVTS
                     GIEVAQRIESYAPESGDGPPTTDVTINSVTITETPKGGAATTAPTTTES"
     gene            1039..2289
                     /locus_tag="VFX21_03925"
     CDS             1039..2289
                     /locus_tag="VFX21_03925"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX5095131.1"
                     /translation="MNASGNASGLVVQKYGGTSVGDPDRVRAVADHVVRTRRSGRGVV
                     VVLSAMGKTTDDQIRLAHDVAGHGNGGREMDMLLTAGERISMSLLCMAVESLGVPAVS
                     FTGSQAGIVTDTAHGKAKILEVRADRIRDALADGRVAIVAGFQGVSTDRDVTTLGRGG
                     SDTTAVALAAALGADECEIYTDVEGVYSADPRIVPAAHRLSHVSFDEMLEMAATGGRV
                     LALRSVEFARNHNVRVHVRSSFTWAPGTWVTEEDRSMEQAIISGVTHDTSEAKITIEQ
                     VPDRPGIAASVFRTLADAGVNVDMIVQNVSTEGHTDISFTLPRVDLGVAATEMDKIIV
                     ETEAAGYRTDEQIGRVSLIGAGMKTNPGVAAKMFETLANEAINIEMISTSTIRISCVV
                     HEDDVERAVRTLHTAFELDEGAVR"
     gene            2286..>2584
                     /locus_tag="VFX21_03930"
     CDS             2286..>2584
                     /locus_tag="VFX21_03930"
                     /inference="COORDINATES: protein motif:HMM:NF013298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX5095132.1"
                     /translation="MSGGVRVGLMGATGLVGTEMLRLLIERDFPVAELHPYASPRSEG
                     RRLPFGDREVVCEVLRDGCFDGLDIVIVDVDDPLALEWAPVAAAAGARVVDNSAA"
BASE COUNT          388 a          844 c          896 g          456 t
ORIGIN      
        1 cgcgtcgagc gtgactccgt cattgtcgga acgggcgagg gacaccgcgg cgatcgcgcc
       61 gccgatcccg accagcagca cgacggcgat caacgcccag atccacggcg cgatgccgcg
      121 cggctgcgac ggcggttgtt cccattgcgt ggacacggtc agctctccgt cgtggtcggt
      181 gccgtcgtcg cggcaccgcc cttgggcgtc tcggtgatcg tgacgctgtt gatcgtcaca
      241 tcggtcgtcg gggggccgtc accgctctcg ggcgcgtacg actcgatcct ctgtgcgacc
      301 tcgatgcctg acgtgacgat gccgaggtac ccgtagtcag gagtgagcga agttccgtca
      361 ccggtcacga tgaagaattg cgagcccgca gtgccggccg cctcgttgcc ggccttggcc
      421 cacgccaccg accccatgac gtagcccgca ccgttcggtg gcagttcggc ttgtactgaa
      481 tagccgggtc cacctgctgt cgtgttgttg gggctcccgg tctgtatgac gaaacccttc
      541 gcggcgcggt tgaacatgag cccgtcgtag aacccgttgc gcgcgaggaa cacgaagttg
      601 ttgacgctcg taggcgcgtt gacgccgtcg agagacatct cgatgtcgcc ctcgctcgtg
      661 ctgatcgtcg cgctgtaatc attggcgtcg gcgtcgatcg ccatcggtgg cggcgcgtcg
      721 aacgtgggtc gatccgaggc gaccgtggtc ggtgttgtcg tcgtggtgct gtcgctagcg
      781 ctcgagttgt cgtcgtcgcg cgtgatgaac gagatgaacg cgatgatcag gatcagcacg
      841 aggaacgcgg atccgaatcg gatcacggcc gtccgccgct tctcccgctt ggctgcggcc
      901 gcccgcgctt cgcgcgcgac gcgtgcgttc tctttcttcc tggtgcgttt cgcccggctc
      961 acgaggtcgg cagcttaccg gtcacccctt ccgaaactcg ggcaaggggc cgcatatgcg
     1021 ctggtagcgt ggtcggccgt gaacgcatcg gggaacgcct ccgggctggt cgtgcagaag
     1081 tacggaggca cttccgtcgg cgaccccgac cgcgtccgcg ccgtcgccga ccacgtggtg
     1141 cgcacccggc gcagcgggcg tggcgtcgtg gtggtcctgt cggccatggg caagacgacc
     1201 gacgatcaga tccgcctcgc ccacgacgtc gccggccatg gcaacggtgg ccgcgagatg
     1261 gacatgctgc tcactgcggg cgagcgcatc tccatgtcgt tgctctgcat ggctgtggag
     1321 tcgctgggag ttcccgcagt gtcgttcacc ggctcgcagg ccggcatcgt caccgacacc
     1381 gcgcacggca aagcgaagat cctcgaggtc cgcgccgacc gcatccgcga cgcgctcgcc
     1441 gacggtcggg tcgcgatcgt cgccggcttc caaggggtct ccaccgaccg cgacgtcacc
     1501 acgctcggcc gcggcggctc cgacaccacc gcggttgcac ttgccgccgc gctcggcgcc
     1561 gacgaatgcg agatctacac cgacgtcgaa ggcgtctaca gcgcggaccc gcgcatcgtg
     1621 cccgccgcgc accggctgtc gcacgtctcg ttcgacgaga tgttggagat ggctgcgaca
     1681 ggcggccgcg tgctcgcgct gcgttctgtc gagttcgcgc gcaatcacaa tgtgcgcgtg
     1741 catgttcgtt cgagtttcac gtgggcgccg ggcacgtggg tcaccgagga ggaccggtcg
     1801 atggagcagg cgatcatctc gggtgtcacg cacgacacgt ccgaggcgaa gatcacgatc
     1861 gagcaggtcc ccgaccgccc tggcatcgcg gcgtcggtgt tccgcacgct cgcggacgcg
     1921 ggtgtgaacg tcgacatgat cgtgcagaac gtctcgaccg agggccacac cgacatctcg
     1981 ttcacgttgc cgcgcgtcga cctcggtgtc gcggccaccg agatggacaa gatcatcgtc
     2041 gagaccgaag ccgccggcta ccgcaccgat gagcagatcg ggcgggtgtc gctgatcggc
     2101 gcgggcatga agacgaaccc gggtgtcgcg gcgaagatgt tcgagacgtt ggcgaacgag
     2161 gcgatcaaca tcgagatgat cagcacgtcg acgatccgta tctcgtgtgt cgtccacgaa
     2221 gacgacgtcg aacgcgcggt gcgcacgctg cacacggcgt tcgagctcga tgaaggggca
     2281 gtgcgatgag tggcggtgtg cgcgtcgggt tgatgggcgc gaccggtctg gtgggcaccg
     2341 agatgctgcg cctgttgatc gagcgtgatt tcccggtcgc cgagctgcac ccgtacgcgt
     2401 cgccgcgatc cgaaggccgg cggttaccgt tcggcgaccg cgaagtcgtg tgcgaagtcc
     2461 tgcgcgacgg atgcttcgac gggctcgaca tcgtgatcgt cgacgtcgac gacccgctcg
     2521 cgctcgagtg ggcgccggtc gccgcggcgg cgggcgcgcg tgtcgtcgac aactcggccg
     2581 cgtt
//
LOCUS       DASUGA010000200         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1439125, whole genome shotgun sequence.
ACCESSION   DASUGA010000200 DASUGA010000000
VERSION     DASUGA010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439125"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VFX21_03935"
     CDS             complement(<1..434)
                     /locus_tag="VFX21_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX5095133.1"
                     /translation="MIPRYSLPEIANLFTDEARFGAWLDIEILAVEAWAQLGVIPEEH
                     AQAVRARADFEVDAIHEREKITDHDVAAFVDVVQAHIGAPAGTWVHYGLTSSDVVDTA
                     LSLQITRALDHIIRAAGDLEAAIAQRAREFRDTPMVGRTHGI"
     gene            complement(431..1726)
                     /gene="purD"
                     /locus_tag="VFX21_03940"
     CDS             complement(431..1726)
                     /gene="purD"
                     /locus_tag="VFX21_03940"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006141431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX5095134.1"
                     /translation="MARVLLVGSGAREHALAWALSRSPHVDALVSAPGSDGTAEFSER
                     RAVDANDARAVADLADEIGADLVVVGPEEPLVKGAASAVRERGRLAFGPGTEAATLEG
                     SKAWMKDVLVAGGVPTARHATFAGPDDADRAYEFLETLPGLYVVKTDGLAGGKGVIVT
                     ESMTEAREAVRAYLTGNAFGDAGRTCVIEEGLTGPELSVFALCDGRHAVAFAAAQDHK
                     RAFDNDEGANTGGMGAYSPVPFAPDSLVDEVMERAIRPTLAELARRNAEYRGVLYCGL
                     MLTPDGIKVIEYNIRFGDPECQVLMPRLASDIYEHCYEAAAGRIETPVRMRDDACIGI
                     VLAADGYPPAPVRTGDVISGLDAASAVEGVQIFHSGARREGDDLVTNGGRVLTLTALA
                     PSIKEARDRGYEAAAKISWPGLHYRRDIGAQALSPDPLG"
     gene            complement(1726..>2457)
                     /locus_tag="VFX21_03945"
     CDS             complement(1726..>2457)
                     /locus_tag="VFX21_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEX5095135.1"
                     /translation="RYLSYVPRLEPMIDDTVHLVHEALEAQQWVLFEGAQATFLDLDH
                     GTYPFVTSSNPIAGGACTGAGVGPRAIERIVGVVKAYVTRVGSGPFPTELHEGDEVGD
                     LLIERGHEFGTNTGRRRRAGWLDLVMLKHAVRLNTATELAMTKLDILSPLEEVKVCVA
                     YEDETGKRYDHVPYHQSVLHKVTPVYETLPGWKSDIDGAARIEDLPPAARDYVKFIEE
                     FAGVPASFVGVGPARDQTLVRPRAA"
BASE COUNT          352 a          842 c          871 g          392 t
ORIGIN      
        1 tggatgccgt gcgtgcggcc gaccatcggc gtgtcgcgga actcacgggc gcgttgcgcg
       61 atcgcggctt cgaggtcgcc ggcggcgcgg atgatgtggt cgagcgcgcg cgtgatctgg
      121 agcgacagcg cggtgtcgac gacatcgctc gacgtcaggc cgtagtgcac ccacgtgccc
      181 gcgggcgcac cgatgtgcgc ctggacgacg tcgacgaagg cggcgacgtc gtgatcggtg
      241 atcttctcgc gctcgtggat cgcgtcgact tcgaagtcgg cgcgcgctcg cactgcctgc
      301 gcatgctctt cggggatcac accgagctgc gcccacgctt cgaccgcgag gatctcgatg
      361 tcgagccacg cgccgaagcg agcttcgtcg gtgaacaggt tggcgatctc gggcagtgag
      421 tagcggggaa tcatccgagt ggatcgggcg agagggcctg ggctccgata tcgcgacggt
      481 agtggagacc cggccacgag atcttggccg cggcctcgta gccgcggtcg cgggcttcct
      541 tgatgctggg cgcgagggcg gtgagggtaa gcacccgtcc accgttggtg accagatcgt
      601 cgccttcgcg gcgcgcgccg ctgtggaaga tctgcacacc ctcgacggcg gacgcggcgt
      661 cgaggccgct gatgacgtca cccgtgcgga cgggtgcggg cggatagccg tcggcggcca
      721 gcacgatgcc gatgcacgcg tcgtcgcgca tgcgcaccgg cgtctcgatg cgtccggccg
      781 cggcttcgta gcagtgctcg tagatgtcgg acgcgagccg cggcatgagc acctggcact
      841 cggggtcacc gaagcggatg ttgtactcga tgaccttgat gccgtcgggt gtgagcatga
      901 gcccgcagta gagaacgccg cggtactcgg cgttgcggcg cgccagctcc gcgagcgtcg
      961 gtcggatcgc gcgctccatg acctcgtcga cgagcgagtc gggggcgaac ggtacgggcg
     1021 agtacgcgcc catgccgccg gtgttcgcgc cttcgtcgtt gtcgaacgcg cgcttgtggt
     1081 cttgcgcggc tgcgaacgcg accgcgtggc gtccgtcgca cagtgcgaac acggacagct
     1141 cgggcccggt gaggccttct tcgatcacac acgtgcggcc cgcgtcaccg aacgcgttgc
     1201 ccgtcaggta cgcgcgcacc gcttcgcgcg cttcggtcat cgactcggtg acgatcacgc
     1261 ccttgccgcc cgcgagcccg tcggtcttga cgacgtacaa gccgggcagt gtctcgagga
     1321 actcatacgc ccggtcggcg tcgtcggggc ccgcgaacgt cgcgtgccgc gcggtgggca
     1381 ccccgcccgc gacgaggacg tctttcatcc acgccttcga tccttcgagc gtggccgcct
     1441 cggtacccgg cccgaacgcg agccggcctc gttcgcgtac tgcactcgca gcgcccttga
     1501 ccagcggctc ctcgggcccg acgacgacga ggtcggcgcc gatctcgtcc gcgaggtcgg
     1561 cgaccgcgcg ggcgtcgttg gcgtcgacgg ctcgccgctc gctgaactcc gcggtgccgt
     1621 cgctgcccgg cgcactgacg agcgcgtcga cgtgcggaga acgcgacagc gcccacgcga
     1681 gcgcgtgctc acgagcaccc gaaccgacga gcaggacgcg tgccatcagg cggcgcgggg
     1741 acgcacgagg gtctggtcgc gcgcgggacc gacgccgacg aacgacgcgg ggacgccggc
     1801 gaactcctcg atgaacttca cgtagtcgcg cgccgcgggc ggcaggtctt cgatgcgcgc
     1861 ggcgccgtcg atgtccgact tccagccggg cagtgtctcg tacaccggtg tcaccttgtg
     1921 cagcaccgac tggtggtacg ggacgtggtc gtagcgcttg ccggtctcgt cttcgtacgc
     1981 gacgcagacc ttcacctctt ccagtggcga caggatgtcg agcttcgtca tcgcgagctc
     2041 ggtcgcggtg ttgaggcgca ccgcgtgctt caacatgacg aggtcgagcc aaccggcccg
     2101 ccgccgccgg ccggtgttgg tgccgaactc gtgcccgcgc tcgatcagca ggtcgccgac
     2161 ctcgtcacct tcgtgcaact cggtggggaa cgggcccgat ccgacacgcg tcacatacgc
     2221 cttgacgaca cccacgatgc gctcgatcgc gcgcgggccg acgcccgcgc ccgtgcacgc
     2281 accgcccgcg atcgggttgc tcgacgtcac gaacggatac gtaccgtggt cgagatcgag
     2341 gaacgtcgcc tgcgcgccct cgaacagcac ccactgttgc gcctccagcg cctcgtgcac
     2401 gaggtgcacg gtgtcgtcga tcatcggctc gaggcgcggc acgtacgaca agtaccg
//
LOCUS       DASUGA010000201         5326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1006202, whole genome shotgun sequence.
ACCESSION   DASUGA010000201 DASUGA010000000
VERSION     DASUGA010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006202"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(314..1837)
                     /locus_tag="VFX21_03950"
     CDS             complement(314..1837)
                     /locus_tag="VFX21_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095136.1"
                     /translation="MRFRRGAAVLVSMVLGAGLLTACKVIPQVAPQATGCSPLAVPSQ
                     EPATVEAPSAPAVPPDDRVNVAVELSTGAPNLDLTGVVWNSGSSIEPLAAAHPSIVRI
                     DGSLQSRSQGPDQLDLQPLLDRVAQVRDIGAEPLVLLSYMPSWLGAPRATNGQDPTRM
                     APYDLDLWQDLVTEVVRTLATAEKPAYRFETWNEPDLPIFWQDTRESFIATALRSHQA
                     VVDVAAETGLPLEIGGPASFHGGSTLLPEYARAVADAGLPLDFVTWHNYANFPFLGPD
                     GPEGNLDPEIYAGLAKRNPNSTPLTYSNEIRDVRKKIKTAVGDSGLSPELIIDEWNVS
                     AGGYDVRHDTAEGASLIAGILVEMERAGLDEAAFYRAVSGSHIGDWGLVYSDGTPKPS
                     WWVFRAWSTMTGDRLFAAGDDATTGLWARATRDGGCVSVLLANFIATGAPARNVEVNL
                     HGELPPCQGQRTTTLATLDATSTNLADTATLHPANSDAITVPMASQSVSLIRISCGR"
     gene            1958..2509
                     /locus_tag="VFX21_03955"
     CDS             1958..2509
                     /locus_tag="VFX21_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019929594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5095137.1"
                     /translation="MPPDPPRTPKAARTRARLHAAAWQLFIERGYHAVSMRDISAAAG
                     LTKTGAYGHFRSKGQLLVEVIRWKLAERDAGIDFSVVDDLVSGVDLMFDDHYREIRLL
                     EVDAAAAARHDPDVAAGLTLLYTERVGRMRDALSAMRDPETAAWIMSALMGGVGMKDS
                     AALPRPDPDRLAAALVAAFAALA"
     gene            2551..3507
                     /locus_tag="VFX21_03960"
     CDS             2551..3507
                     /locus_tag="VFX21_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5095138.1"
                     /translation="MDASRPCGPGSVSLRCYPHLELDAPEILAEMRAQAALAAEHGFD
                     GVMTSEHHGGFAGYLPNPLQLAGFLLDGMPRGWAAACPVLATLRPAALIAEETAWLAA
                     RYPERVGVGLAAGALAQDFEVMDLDMHDLTARFTRALERVCALLRGETDGVLAGDPAI
                     ARCREHPIPVVSAAASATAARRAARVGAGIILDSLTSVARCRELTDAYRGAGGTEAVV
                     LVRRAWIGEPPRDLFERQVSVYRGYASPDASATWRADEMLVDTDGDALAARIEQTVRA
                     AGADACNVRIHVPGIDAARVRTQITRLGQDVVPTLRDALRAA"
     gene            complement(3515..3997)
                     /locus_tag="VFX21_03965"
     CDS             complement(3515..3997)
                     /locus_tag="VFX21_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthrone oxygenase family protein"
                     /protein_id="HEX5095139.1"
                     /translation="MIDGFPRVVATTAAIAAAVNAGVFFAFSTFVGRALIDLPASQGI
                     ATMQSINRRAPTPWFMLLFMGTALLAVVSIAVGIGRWSEVWGAYVVIGGALYLVSIVM
                     TAAYHQPRNLALDDVDANGPDAAATWTAYIHPWIAWNHVRTLVCIGSALAFVLSLRVD
                     "
     gene            complement(3997..4119)
                     /locus_tag="VFX21_03970"
     CDS             complement(3997..4119)
                     /locus_tag="VFX21_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095140.1"
                     /translation="MERLGREAFHGRMSLDTAPALQPTLVLGAGGKTGRQQEDG"
     gene            4272..4697
                     /locus_tag="VFX21_03975"
     CDS             4272..4697
                     /locus_tag="VFX21_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020121629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HEX5095141.1"
                     /translation="MADDAMGPIGYLIVEYPGNKMTGEGLIELVNLVDQGLIRVLDLI
                     FVTRDDDGIRAVELTDLDGDGTIDLAVFEGASSGLLDQSDLEDAAAAIEPNSSAAILV
                     YENRWAIGLVQALRDSEAQVVAAGFIPQDALIESLDALD"
     gene            4723..5016
                     /locus_tag="VFX21_03980"
     CDS             4723..5016
                     /locus_tag="VFX21_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003935456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX5095142.1"
                     /translation="MPGLMRGVARTAVIAGTATAVSNRVSRRQANKWSAQEEQQYQAQ
                     QYQQQQAAAAAPPPAAPQEDMVEQLQKLASLKDQGILTEEEFAAQKAKILAAQ"
BASE COUNT          843 a         1810 c         1845 g          828 t
ORIGIN      
        1 gcggcgctgt cgtcggattc ggcacgggtg atacgtacac cggcaccaac gggatagccg
       61 ggtcgatcgg agagcgcacc gatggcacgt gtcgcgttgt ctgtgagcgt cagcatgacg
      121 tttcccttca gctgcgcgcg aacaagcact cgtgtctgtt cgcggtcacg catcgcggaa
      181 tcgaaccacg cgcgttcccg ccggtgccgt tcatgaaaca cggacgccgg aaacgggtgc
      241 ggagccggtt aggcgtggcg catcgcctgc aacagtgtgt ccggtgacgg tcggcgcgcc
      301 gaccgtggac gtttcaacgt ccgcagctga ttcggatcaa tgagaccgat tgcgatgcca
      361 tcggcaccgt gatcgcgtcg ctgttcgcag gatgaagggt cgccgtgtct gcgaggttcg
      421 tagacgttgc gtcaagcgtc gcgagcgtgg tcgtgcgttg gccttggcac ggcggcagtt
      481 cgccgtggag gttcacttcc acattgcgcg cgggtgcgcc ggtggcgatg aagttggcga
      541 gcagaaccga gacgcatccg ccatcgcgcg ttgcgcgggc ccacagaccg gtcgtggcgt
      601 cgtcgcccgc ggcgaagagt cggtcaccgg tcatcgtcga ccacgcgcgg aagacccacc
      661 aggacggctt cggagtcccg tcggagtaca cgaggcccca gtcgccgatg tggctcccgg
      721 acaccgctcg gtagaacgcg gcctcgtcga gaccggcgcg ttccatctcg acgaggatcc
      781 cggcgatcag cgacgcgcct tcggcggtgt cgtggcgcac gtcgtagccg cccgccgaga
      841 cgttccactc gtcgatgatg agctcgggag agagtccgct gtcgccgacc gcggtcttga
      901 tcttcttgcg gacgtctcgg atctcgttcg agtacgtgag cggcgtcgag ttcgggttcc
      961 gcttcgcgag ccctgcatag atctcggggt cgagattgcc ttccggcccg tccgggccga
     1021 ggaacgggaa gttggcgtag ttgtgccacg tcacgaaatc gagcggcagc ccggcgtcgg
     1081 ccacggctcg cgcgtactcc ggcagcagtg ttgacccgcc gtggaacgac gcggggccgc
     1141 cgatctcgag cgggaggccg gtctcggccg caacgtcgac gacggcctgg tgcgatcgca
     1201 gcgcggtcgc gatgaaggac tcgcgagtgt cctgccagaa gatcgggagg tcgggctcgt
     1261 tccacgtctc gaagcggtag gccggtttct cggccgtcgc cagtgtccgc acgacctcgg
     1321 tgacgaggtc ctgccagagg tcgaggtcgt agggggccat ccgcgtcggg tcctgaccgt
     1381 tcgtcgctcg gggcgcgccc agccagctcg gcatgtagga caggagcacg agcggctcgg
     1441 cgccgatgtc gcgcacctgc gcgacgcggt cgagcagcgg ctgcaggtcg agctgatcgg
     1501 gtccctggct gcgactctgc aagctcccgt cgatgcggac gatcgacgga tgcgcggctg
     1561 ccaacggttc gatgctgctg ccggagttcc acaccactcc ggtcaggtcg aggttcggag
     1621 ctccggtcga cagttcgacg gcgacgttca cacggtcgtc cggcggcacg gcgggcgcgc
     1681 tcggcgcctc gacggtggcc ggctcctgac tggggacggc gaggggcgag caacccgttg
     1741 cctgcggcgc gacctggggg atcaccttgc aggccgtgag cagcccggcg ccgagcacca
     1801 tgctcacgag caccgctgca ccgcgacgga atcgcatgcc gtctccctgc ctcgtcagtt
     1861 attgagcgcc ggactataat gcggtgctca attctgtcaa ccgcgaggag acgtcctgcc
     1921 cggcgaagcg gtgcaagacg cggcgacggg ctccttcgtg ccgccggacc cgccgcgaac
     1981 tcccaaagcc gcgcgtacgc gcgcccgcct ccacgcggcc gcctggcagc tcttcatcga
     2041 acgcggatac cacgcagtgt cgatgcgaga catctcggcc gcggccggtc tgaccaagac
     2101 cggcgcgtac ggacacttcc gctcgaaggg ccagctcctc gtcgaagtga tccggtggaa
     2161 gctcgccgag cgcgacgccg gcatcgactt cagcgttgtc gacgacctcg tgagcggcgt
     2221 ggacctgatg ttcgacgacc actaccgcga gattcggttg ctcgaggtcg atgcggccgc
     2281 ggccgcgcgc cacgaccccg acgtcgccgc gggcctgacg ttgctctaca ccgagcgagt
     2341 cggacgaatg cgcgacgcgc tctcggcgat gcgtgacccc gagactgccg cgtggatcat
     2401 gagtgcgctc atgggagggg tcgggatgaa ggactccgcg gcgcttccgc ggcccgatcc
     2461 ggaccgactc gccgccgcgc tcgtcgcggc ctttgccgcg ctcgcctgac gccggaccgc
     2521 gaacccgagg cgtcgttacg atgcgcggcc gtggacgcca gtcgaccctg cgggccgggg
     2581 tcggtctcct tgcgctgtta cccgcacctc gaactggacg cgccggagat cctcgccgag
     2641 atgcgcgccc aagccgcgct cgcggccgaa cacggcttcg acggcgtgat gaccagcgag
     2701 catcacggcg gcttcgcggg ctacctgccg aacccgttgc agctggccgg cttcctcctt
     2761 gacggcatgc cgcgcggttg ggccgccgcg tgcccggtgc tcgcgacgct gcgccccgcc
     2821 gcgttgatcg cggaggagac ggcgtggctc gcggcccgct atccggaacg ggtcggcgtc
     2881 ggtctcgccg cgggcgcgct cgcgcaggac ttcgaggtga tggacctcga catgcacgac
     2941 ctgacggcgc gcttcacccg cgcgctggaa cgggtgtgtg cgctgttgcg gggcgagacc
     3001 gacggtgtac tcgccggcga ccccgccatc gcgcgttgtc gcgaacatcc gattccggtc
     3061 gtcagtgcgg cggcgagcgc aacggccgcg cgccgcgccg cccgcgtcgg cgcgggcatc
     3121 attctggact cactcaccag cgtggcgcgg tgtcgtgagc tgaccgatgc gtaccgcggc
     3181 gcgggtggca ccgaggcggt cgtgctcgtc cgtcgcgcgt ggatcggtga accgccgcgc
     3241 gatctcttcg agcgccaggt gagcgtctat cgcggctacg cctcgcccga cgcgtcggcg
     3301 acttggcgtg cggacgagat gctcgtcgac accgatggtg acgccttggc ggcccgcatc
     3361 gagcagacgg ttcgcgccgc gggcgccgac gcgtgcaacg tgcgcatcca cgtccccggt
     3421 atcgacgcgg ctcgcgtccg gacgcagatc actcgcctcg gccaagacgt cgtgccgacg
     3481 cttcgggatg cgctgcgggc ggcgtgaggg aacctcagtc gacgcgcaac gacagtacga
     3541 acgcaagcgc acttccgata cagacgagcg tgcgtacgtg gttccacgcg atccacggat
     3601 ggatgtacgc ggtccacgtg gccgccgcat cgggaccgtt tgcgtccacg tcgtcgagcg
     3661 ccaggttgcg cggctggtgg taggccgcgg tcatcacgat cgacacgaga tacaacgcgc
     3721 caccgatcac gacgtacgcg ccccacacct cgctccaccg gccgatgccc accgcgatcg
     3781 agaccacggc gagcagcgca gtgcccatga acaacaacat gaaccacggc gtcggagccc
     3841 ggcggttgat ggactgcatc gtcgcgatgc cctgcgatgc cgggaggtcg atgagggcgc
     3901 ggccgacgaa ggtcgagaac gcgaagaaca cacccgcgtt gaccgccgcc gcgatggcgg
     3961 cagtcgtcgc gaccacgcgg gggaacccgt cgatcatcag ccgtcctcct gctgccggcc
     4021 ggtcttgccg cccgcgccga ggaccaaggt cggttggagg gcaggtgcag tgtcgaggct
     4081 catgcgtcca tggaacgcct cccggccgag acgttccacg gtgcaagaac tcaaatacat
     4141 acgcgaacga tgtgaacccg acatgacgat cggtcccgcc gccagttcac ccggtccgaa
     4201 tgatgacggg gcaggtcggg cgtgcgagac tgccggtcca catccgttcg acatcgaggg
     4261 tggtgccgca catggccgat gacgcaatgg ggccgatcgg ttatctgatc gtcgagtatc
     4321 ccggcaacaa gatgaccggc gaggggctca tcgagctcgt caatctcgtc gaccagggcc
     4381 tgatccgagt cctcgacctg atcttcgtga cccgcgacga cgacggcatc cgcgccgtcg
     4441 agctgaccga cctcgacggc gacggcacga tcgacctggc cgtcttcgaa ggcgcctcgt
     4501 cgggactgct cgaccagagc gacctcgagg atgccgccgc cgcgatcgag ccgaactcgt
     4561 cggccgcgat cttggtctac gagaaccgct gggcgatcgg gctcgtgcag gcgctgcgcg
     4621 actccgaggc gcaggtcgtc gcggccggtt tcatccccca agacgcactc atcgagtcgc
     4681 tcgacgcgct cgactgacca accgaacgag gagctgacac caatgcccgg actcatgcgt
     4741 ggggttgccc gcaccgctgt catcgccggt accgccactg cggtctcgaa ccgtgtctca
     4801 cgccggcagg cgaacaagtg gtccgctcag gaagagcagc agtaccaggc gcagcagtac
     4861 caacagcagc aggccgcggc cgccgcaccg ccgccggccg cgccgcaaga agacatggtc
     4921 gagcagctcc agaagctcgc ctcgctgaag gaccaaggca tcctcaccga agaggagttc
     4981 gcggcgcaga aggcgaagat cctcgccgcg cagtagttcc cgagcggagc ccgccgtgct
     5041 gttcctctac cggcctcgtc agacgtacat gccctaccgg gtaccgcgcg cgatctcgca
     5101 acagaccgcg tacaaccggg agatgcaggc gaagttcgac gcgacgcgtc gcgtgccgcc
     5161 gccgagtgct cgtcctcccg ccgcgcgatg acgtcgaacg tcgagcgcgc cgagatactg
     5221 acgcgcgcgc tcgacgcggg tatccgtggt gacttcgcgg cactcgaagg gctgtacacg
     5281 gacgacatca aggggtgggg tgctgcgatc tcggcgtcgt cggccg
//
LOCUS       DASUGA010000202         2269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_32563, whole genome shotgun sequence.
ACCESSION   DASUGA010000202 DASUGA010000000
VERSION     DASUGA010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32563"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VFX21_03985"
     CDS             <1..639
                     /locus_tag="VFX21_03985"
                     /inference="COORDINATES: protein motif:HMM:NF016051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEX5095143.1"
                     /translation="VRVLVTAGGTREPVDAVRVLSNRSSGKQGYAIAAAAAKRGAVVT
                     LVTTVDRPVPPGMAQVVRVETAAQMEAAVMPIAPDQDVIVMAAAVADFRPKAPADRKT
                     KKEEGPPELILEPTHDFLVDLGARKSGHQVLVGFAAETDDLVANAADKLARKGLDLIV
                     GNDVGVSDSGFEADTNRAVLLDRSGGTETLGLLSKDAVAERLLDRIETLRSA"
     gene            772..1968
                     /gene="metK"
                     /locus_tag="VFX21_03990"
     CDS             772..1968
                     /gene="metK"
                     /locus_tag="VFX21_03990"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX5095144.1"
                     /translation="MSKHTFTSESVTEGHPDKMSDQISDSVLDAVLTEDPYGRVACET
                     MVTTGLCVVAGEISTKAHIDFSRLARDTICEIGYTDAAYGIDGKTCGVVVSIDEQSPD
                     IAMGVDKALEQKADSADAYDEVGAGDQGMMFGYACDETPDLMPMPIWLAHKLAARLTE
                     VRKQDIVKFLRPDGKTQVSLDYEDGVPKRLNTVLISTQHDPGISIEHDLRPALIEHVI
                     TPSLPEQFRGDDFEVFVNPTGNFELGGPHADCGLTGRKIIVDTYGGMARHGGGAFSGK
                     DPTKVDRSAAYAVRQIAKAVVAAGLAQRCEAQVAYAIGVAHPVSIMVDTFGTGKVADD
                     KLEGFVREVFDMRPAAIIERLDLRRPIFRRTAAYGHFGRTDSTGGFTWENVDTYAKTL
                     REAAGA"
     gene            1914..>2269
                     /locus_tag="VFX21_03995"
     CDS             1914..>2269
                     /locus_tag="VFX21_03995"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX5095145.1"
                     /translation="MGERRHLRQDTARGGGGIAPRSRACRVVPDVTALAREFDYLVPE
                     EFAARVRVGTIVRVPLHGRKVRGWVVEDDVAPRDGLLPLAAVVSDGPPADVVALTDWI
                     AWRWAGPRVAVLRSASP"
BASE COUNT          373 a          804 c          778 g          314 t
ORIGIN      
        1 gtgcgcgtgc tcgtgaccgc gggcggtacc cgcgagcccg tcgacgcggt gcgcgtgctc
       61 tccaatcgct cgtcgggcaa gcagggctac gcgattgccg cggccgccgc gaagcgcggc
      121 gcagtcgtca ccctcgtcac caccgtcgac cggccggtgc cgccgggcat ggcccaggtg
      181 gtgcgggtcg agaccgcggc gcagatggaa gcggcggtca tgccgatcgc gcccgaccaa
      241 gacgtcatcg tgatggccgc cgccgtcgcc gacttccggc ccaaggcgcc cgccgaccgc
      301 aagacgaaga aggaagaggg cccccccgag ctgatcctcg agccgaccca cgacttcctc
      361 gtcgacctcg gcgcccgcaa gtcgggccac caggtgctgg tcgggttcgc ggccgagacc
      421 gacgatctgg tcgccaacgc ggccgacaag ctggcgcgca agggcctcga cctgatcgtc
      481 ggcaacgatg tgggcgtttc ggactccggt ttcgaggccg acacgaaccg ggcggtgctg
      541 ctcgaccgta gcggcggcac cgagacgctc gggttgttgt ccaaggacgc cgtcgccgag
      601 cggctgctcg atcgcatcga aacgctccgc tcggcctgaa ccccaacttc gctctacgat
      661 gcgcccacct cgtccaacaa cggcgccgaa cggagtgagg ctgcccggag cggagggcgg
      721 gccaacaagg cgacgtacct cacgcgagca cgaccggagc gacaaagaga catgagcaag
      781 cacaccttta cctcggagtc ggtgaccgag ggtcaccccg acaagatgtc ggaccagatc
      841 agcgacagcg tgctcgacgc ggtgctgacc gaggacccgt acggccgggt cgcctgcgag
      901 acgatggtga ccaccggtct gtgcgtcgtc gccggcgaga tcagcaccaa ggcgcacatc
      961 gacttctcgc gcctggcccg cgacacgatc tgcgagatcg gctacaccga cgccgcgtac
     1021 ggcatcgacg gcaagacctg cggtgtcgtc gtgtcgatcg acgagcagtc acccgacatc
     1081 gcgatgggtg tcgacaaggc gctcgagcag aaggccgaca gcgccgacgc gtacgacgag
     1141 gtcggcgccg gtgaccaggg catgatgttc ggctacgcct gcgacgagac gcccgacctc
     1201 atgccgatgc cgatctggct cgcgcacaag ctcgcggcgc gcctgaccga agtgcgcaag
     1261 caggacatcg tgaagttcct gcggcccgac ggcaagacgc aggtgtcgct cgactacgaa
     1321 gacggcgtcc cgaagcggct caacacggtg ctgatctcga cgcagcacga cccgggcatc
     1381 agcatcgagc acgacctgcg gcccgcgctc atcgagcacg tgatcacgcc gtcgctgccc
     1441 gagcagttcc gcggtgacga cttcgaagtg ttcgtgaacc cgaccggcaa cttcgagctc
     1501 ggcggtcctc acgccgactg cgggctcacg ggccgcaaga tcatcgtcga cacgtacggc
     1561 ggcatggctc gccacggtgg tggcgcgttc tcgggcaagg acccgacaaa ggtcgaccgc
     1621 tcggccgcgt acgcggtccg tcagatcgcc aaggcggtcg tcgccgccgg gctcgcccag
     1681 cgctgcgagg cgcaggtcgc gtacgccatc ggtgtcgccc acccggtgtc gatcatggtc
     1741 gacaccttcg ggaccgggaa ggtcgccgac gacaagctcg aaggcttcgt gcgtgaggtc
     1801 ttcgacatgc gcccggccgc gatcatcgaa cggctcgacc tgcgccgccc gatcttccgc
     1861 cgcaccgccg cgtacggcca cttcggccgc accgacagta cgggcggctt cacgtgggag
     1921 aacgtcgaca cctacgccaa gacactgcgc gaggcggcgg gggcatagct ccccgctcgc
     1981 gcgcctgtcg cgtcgtccct gacgtcaccg cgctggcgcg cgagttcgac tatctggtcc
     2041 cggaggagtt cgcggcgcgg gtgcgcgtcg ggacgatcgt gcgcgtgccg ttgcacggcc
     2101 gcaaagtgcg cggctgggtc gtcgaggacg atgtcgcccc gcgcgacggc ctgctgcccc
     2161 tggcggcggt ggtctccgac ggcccaccgg cggatgtcgt cgcgctgacc gactggatcg
     2221 cctggcgctg ggccggcccg agagtggccg tcctccgcag cgcctcacc
//
LOCUS       DASUGA010000203         2224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_539861, whole genome shotgun sequence.
ACCESSION   DASUGA010000203 DASUGA010000000
VERSION     DASUGA010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539861"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..1165)
                     /locus_tag="VFX21_04000"
     CDS             complement(218..1165)
                     /locus_tag="VFX21_04000"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5095146.1"
                     /translation="MQLWTDEIEGLRAEARAAVESGLDLLPLTRPVDESGMTREERIA
                     HVRGRHQPYYTVDDAIERTIAGVRCRVFVPEGTPRAIYLHFHGGGMVAGAPDMMDIPN
                     RHVARTHRVVVVSADYRKAPEHPWPAGPDDGVAVAAWLLEHGADEFGASRMLIGGESA
                     GGYMTAITALRIRDELGAIDRVDGLNLVFGVYDWGRSPSQRGQRPHDGPDILTVEGIE
                     FFTECFVPGRTDDERRAPEISPAYADLRGLPPCLVSVGTCDHLLDDSLLFATRAAAAG
                     VDVELFVAPDMPHAFLAFDCGITKRWAALTAEWFEARLA"
     gene            complement(1185..1700)
                     /locus_tag="VFX21_04005"
     CDS             complement(1185..1700)
                     /locus_tag="VFX21_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095147.1"
                     /translation="MLRRLVVLCALVALVLVACGGDDDEVVTTPSTNDASGTGGTDGG
                     GGGLSPDAPVSSPPMSDGGQLQPSGAQRVEPQPGMADVRPQVFDTEHPPEAVDGGVLV
                     RFYGGVAPCFVLDHYDVAETRRDVTITLFAGSDPSSPDVACIEIAALYEVMVPLDAPL
                     GDRTVIDGAAP"
     gene            1725..>2224
                     /locus_tag="VFX21_04010"
     CDS             1725..>2224
                     /locus_tag="VFX21_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012274980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX5095148.1"
                     /translation="MTNGFAGRAAIVGIGETDYVRGSPHLPVELMLHAADTAIADAGL
                     TPADIDGIVPPPGYTSSEELAANLGIDNLAFATTVHMGGASSVAAIQHAALAVSSGIA
                     KNVLCVVGWNGYSAFRPREGIPRPRRGLDAGAVGDVVVDFYLPYGARSAAQFYGWIAT
                     RHKQLYG"
BASE COUNT          329 a          792 c          790 g          313 t
ORIGIN      
        1 agtcggcgcg cgacgacgtc tgcacctgct cgtagagcgt ctggtagtga tggcggggcg
       61 acccgtcctc ggcgaacatc tcgtcccatg cggacgtgag ctggtagccg tcgaggaggt
      121 cgcccacgac ggtcagcgtc gcaggcgcgt cgcgcgcctc agtgtcgcga aggtgaaggg
      181 cgagcgtccg gcatctgcca ccaatgtgac agcgcgctca ggcgaggcgc gcttcgaacc
      241 actccgcggt cagcgcggcc cagcgcttgg tgatgccgca gtcgaacgcg aggaacgcgt
      301 gcggcatgtc gggcgcgacg aagagctcga cgtcgacgcc agcggccgcg gcgcgtgtcg
      361 cgaacagcaa cgagtcgtcg agtaggtggt cgcacgtgcc cacgctgacg aggcacggcg
      421 gcaggccgcg caggtcggcg taggccggcg agatctcggg cgcgcggcgc tcgtcgtcgg
      481 tgcggcccgg gacgaagcac tcggtgaaga actcgatgcc ttcgacggtg aggatgtcgg
      541 ggccgtcgtg cgggcgttgc ccgcgctggc tcggcgagcg cccccagtcg tagaccccga
      601 acaccaggtt gaggccgtcg acgcggtcga tggcgccgag ctcgtcgcgt atgcgcagcg
      661 cggtgatcgc ggtcatgtag ccaccggccg actcgccgcc gatgagcatg cggctcgcgc
      721 cgaactcgtc cgcgccgtgc tcgagcaacc acgcggcaac cgcgacgccg tcgtcggggc
      781 ccgccggcca cggatgctcg ggcgccttgc ggtagtcggc cgacacgacg acgacgcggt
      841 gcgtgcgcgc gacatgccgg ttcgggatgt ccatcatgtc gggcgcgccc gcgaccatgc
      901 cgccgccgtg gaagtgcaga tagatcgcgc gcggcgtgcc ctcggggacg aagacgcggc
      961 accgcacgcc cgcgatcgtg cgctcgatcg cgtcgtcgac cgtgtaatac ggctggtggc
     1021 ggccacgaac atgcgcgatg cgctcctcgc gcgtcatccc cgactcgtcg accggacgcg
     1081 tcagcggcag cagatcgagc ccgctctcca ctgcggcgcg cgcttcggcc cggagtcctt
     1141 cgatctcatc tgtccagagc tgcatggcgt cagtctcgcc gcgctcaggg cgcggcgcca
     1201 tcgatcacgg tgcggtcgcc gagcggcgca tccagaggca ccatgacttc gtacagcgcg
     1261 gcgatctcga tgcaggccac gtcgggcgac gacgggtcgc tccccgcgaa caatgtgatc
     1321 gtcacgtcac gccgggtctc ggccacgtcg tagtggtcga gcacgaagca cggcgcgacg
     1381 ccgccgtaga accgcacgag tacgccgccg tcgaccgctt cgggcggatg ctcggtgtcg
     1441 aagacctggg gccgcacgtc ggccatgccc ggctgcggct cgacccgttg cgcgccgctc
     1501 ggctgcagct gaccgccgtc gctcatcggc ggcgagctca ccggcgcgtc gggcgacaga
     1561 cctcccccac ctccgtcggt accaccggta ccggacgcat cgttggtgct gggagtggtg
     1621 acgacctcgt cgtcgtcacc gccgcaggcg acgaggacga gcgcgaccag cgcacacagc
     1681 acgacaaggc ggcggagcac gatcacagac tacgttcggc cgccgtgacc aacgggttcg
     1741 caggacgcgc tgcgatcgtc ggtatcggcg agaccgacta cgtgcgcggc tcaccgcatc
     1801 ttccggtcga gttgatgctg cacgcggccg acaccgcgat cgccgacgcc gggctcacgc
     1861 cggccgacat cgacggcatc gtcccgccgc ccggctacac gtcgagcgag gagctcgcgg
     1921 ccaacctcgg catcgacaac ctcgcgttcg cgacgaccgt gcacatgggt ggcgcgagct
     1981 cggtcgccgc gatccagcac gccgcgctcg cggtgagctc cggcatcgcg aagaacgtcc
     2041 tgtgcgtcgt cggctggaac ggctactccg cgttccggcc gcgcgaaggc atcccgcggc
     2101 cgcgccgcgg cctcgacgcc ggcgcggtcg gcgacgtcgt cgtcgacttc tacctgccgt
     2161 acggcgcccg ttcggccgcg cagttctacg gctggatcgc gacccgtcac aagcagctgt
     2221 acgg
//
LOCUS       DASUGA010000204         5844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_261740, whole genome shotgun sequence.
ACCESSION   DASUGA010000204 DASUGA010000000
VERSION     DASUGA010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261740"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..783
                     /locus_tag="VFX21_04015"
     CDS             76..783
                     /locus_tag="VFX21_04015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5095149.1"
                     /translation="MTALDPGTWARGRGVWAALAGADVFPESGARVVVNAESQICPPD
                     WCGVVALGDGVLATTPDEARRGRLVEQCNDLGPADDWAIAFADVAEVRGPARLAYIDL
                     RVFPDEDEPPFTEVLRVGDDAVAAFVAGVPAADRDEAGLEGCTSPLACVRHDGAIVAA
                     AGYRIWREELAHMSVLVAPDHRGAGLATTVARDMTRSALEHGLIPQWRARPAASQAVA
                     RKIGYVDVGQQISIKLP"
     gene            797..1345
                     /locus_tag="VFX21_04020"
     CDS             797..1345
                     /locus_tag="VFX21_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX5095150.1"
                     /translation="MPVKEGPIPPFPDGADPDEYDRLRRRVLWKMPSGLYIVGSTDKG
                     DRRNGMTLNWATQLSFDPKWLGIGVEHGAFTHELITAGGVFALCIIDREDRAIVRKFT
                     KPVDVDLAAHTLNEFAYFDGPATGAPVLTQSVAYVECEVRQTVEIGNHSLFLGEVVNA
                     AFLKDEATDVLRMEDTRMNYGG"
     gene            1342..3372
                     /locus_tag="VFX21_04025"
     CDS             1342..3372
                     /locus_tag="VFX21_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095151.1"
                     /translation="MTVRRALVLLVVVFLVGITACSGGDDDDSSASTAPGSTTTTFPP
                     LMGTIEVGELQAPDRCDPIDQRHCLLPFPSDTFTVADETTETGRRVALSRDSMPTNKD
                     GVHVDPREWNRNDGFSPGQPISIYVAGLDVAQSHLAPSTDIGSSLADDAPIVLLDATT
                     GERHPYWSELDASVGSDDARVLYIRPAVNYPDGHRMIVALRGLKDASGAAIEPSAVFR
                     GYRDRLDTKDVSVEARRPRYEQVFGDLEDAGVERDDLYAAWDFTVASTQNLTERMLTI
                     RDDAFADLGDDAPSFTIDAVEEHPEGLFRRVTGTVQVPLYLTGVGDPGSSFHYGDGEN
                     VPSRNGTESFGAGFICNIPEAARTQPARLSLYGHGLLGSNDEVNAGNVRAFAQEHDIV
                     FCATKWIGMSEDDIPNAVAILQDLSKFPTLADRAQQGFLNALFLGRLMQSDAFANDPN
                     LAGLLDTSALYYDGNSQGAIMGGALTAVSQDFTRSVLGVTGMNYSTLLQRSIDWDTYR
                     AVYDPAYPDEIERGIGISLIQMLWDRAEANGYANHMTGDPLPDTPEHTVLLHVALGDH
                     QVANVAAEVEARTIGARIHCPATTDGRLPDVEPHWDLECAKDGDTGSVLVWWDSGTPP
                     APPVNLAPSGGEDPHGDPRSSPDARTQKSEFLQPDGTFVDVCNGQACTAAPS"
     gene            3383..4429
                     /locus_tag="VFX21_04030"
     CDS             3383..4429
                     /locus_tag="VFX21_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEX5095152.1"
                     /translation="MATETPDRTGTQASRDTREWSLGKKWRYVLATTNPPPGRLDPVS
                     KWLYLTRAGVLPMTLVSATVAGLLAVYRDDNVSWGWFALASLGIVLAHMANNLMNDLF
                     DVKVGTDTEDYPRNLYSPHPVLSGVISKKGLATCALVVNAMCFVIMIVLTVARGWAIV
                     AFALGGYILSASYTAPPLRLKKRGLGEPTVLIVWGPLMVGGVYYAATGSIPGAVILAS
                     LPYALLCTTVLMGKHIDKLPWDERDGTRTLPVMLGEARARAITKGMFAVFYASVVALV
                     IAQILPIASLLVFLSAPIMVRTWKAYGEPKPAESPMPNPVWPLWFAPHSFLVTRRAGS
                     LLVLGLLIGAIVGW"
     gene            complement(4355..>5844)
                     /locus_tag="VFX21_04035"
     CDS             complement(4355..>5844)
                     /locus_tag="VFX21_04035"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX5095153.1"
                     /translation="RDCPGTTTCPAGGRCQAEAARVRTQLSEIVVVNTALYGSNIMAG
                     GHVLPPHDAVVIDEAHLSEDVFADQFGVEVHSGRLRHLAALVEPVANAHEAVDRLRRA
                     GKRLDELCTAAAVPEDPRVHACEGPLAEILATIAAAVADALKKLKDAKLDAGSPASAR
                     KTRAEKAAQTLAYEVGRALDPDVYDTHVAWCTTEGSTPRLALMPVVVGDVLSQQLLPT
                     ATVVATSATLAVGGNFDAMAARFGLTADDNWDGDRVPSPFDFREQAMLYVPKHLPDPR
                     APGAADALADELHSLIVAAGGRTLALFTSWNAMRAAADRCAAKGEYEVLRQGDRPRRV
                     LVDSLRDNATAGGVAVFATMGFWTGVDVAGVGLSLVTIDRIPFPRPNEPLHAARRERA
                     VQAGLDPFATVDVPRAAMLLAQGAGRLIRHREDRGVVAVLDRRLATAGYRRPILATMP
                     PFRRSIDGDAVRVFLAEITRALPADDGADQEAEDQEAAGAAGDEE"
BASE COUNT          876 a         2025 c         2016 g          927 t
ORIGIN      
        1 gggcggcctc tcccaacgcg ccacgctcgc gttcgcgctc ggtttctatc ccgcggtcgc
       61 gatcgcggcg acacggtgac cgccctcgat cccgggacct gggcgcgggg gcgcggcgtc
      121 tgggccgcac tcgccggtgc cgatgtgttc cccgaatcgg gcgcgcgcgt cgtcgtgaac
      181 gccgagtcgc agatctgtcc gcccgattgg tgcggcgtgg tcgcgctcgg cgatggcgtc
      241 ctggcgacga cgccggacga agcgcggcgg ggccgtctgg tcgagcagtg caacgatctc
      301 ggccccgccg acgactgggc gatcgcgttc gccgacgtcg ccgaagtgcg cggtccggcg
      361 cggctcgcgt acatcgatct ccgtgtgttc cccgacgaag acgagccgcc gttcacggaa
      421 gtgcttcgag tgggcgacga cgccgtcgcc gcgttcgtgg ctggtgtccc cgccgccgat
      481 cgcgacgaag ccggtctcga gggctgcacg tcaccgctcg cgtgtgttcg ccatgacggc
      541 gccatcgtgg ccgcggccgg atatcgcatt tggcgcgaag aactggcgca catgtctgtg
      601 ctcgttgcgc ccgaccatcg cggcgcgggc ctcgcgacga cggtcgcgcg cgacatgaca
      661 cgctccgccc tcgaacacgg cctgataccg cagtggcggg cgcggcccgc tgcgtcgcag
      721 gcggtggcgc gcaagatcgg ctacgtcgac gtcgggcagc agatctccat caagctgccg
      781 taggtaacgt ggccggatgc ccgtcaaaga aggtccgatt ccgccgtttc cggacggtgc
      841 cgatcccgac gagtacgacc gcttgcggcg gcgcgtgctg tggaagatgc cgtcgggcct
      901 ttacatcgtc gggtcgaccg acaagggcga tcgccgcaac ggcatgacat tgaactgggc
      961 gacacagctg tcgttcgacc cgaaatggct cggtatcggt gtggaacatg gtgcgttcac
     1021 gcacgaactg atcaccgcgg gcggcgtgtt cgcgctgtgc atcatcgacc gcgaagaccg
     1081 tgcgatcgtc cgcaagttca cgaagccggt cgacgtcgat ctggccgcgc acacgctcaa
     1141 cgagttcgcg tacttcgacg gtcccgccac cggcgcgccg gtgctgacac aatccgttgc
     1201 ctacgtggaa tgcgaagtac gccaaacggt cgagatcggc aatcactcgt tgttcctcgg
     1261 tgaggtcgtg aacgccgcgt tcttgaagga cgaagcaacg gatgttctcc gcatggagga
     1321 cacgcgcatg aactacgggg gttgaccgtg cgccgggcgc tcgtgttgtt ggtcgtcgtc
     1381 ttcctggtcg gcatcaccgc gtgcagcggt ggcgacgacg acgactcgag cgcgtcgacc
     1441 gcgccgggct cgacgacgac gaccttcccg ccgctcatgg gcaccatcga ggtcggcgag
     1501 ttgcaggcgc ccgatcgttg cgatccgatc gatcagcggc actgcttgct gccgttcccg
     1561 tccgacacgt tcaccgtcgc cgacgagacc acggagaccg ggcggcgcgt cgcgttgtcg
     1621 cgcgactcga tgcccaccaa caaggacggc gtccacgtcg acccgaggga gtggaaccgc
     1681 aacgacggct tcagtccggg ccaaccgatc tcgatctacg tcgcgggact cgacgtcgcg
     1741 cagtcgcacc tggcgccgtc caccgacatt ggctcgtccc tcgccgacga cgcgccgatc
     1801 gtgctgctcg acgccacgac cggcgaacgg catccgtact ggagcgagct cgacgcgagc
     1861 gtcggcagcg acgatgcacg cgtgctgtac atccggcccg cggtgaacta tcccgacggc
     1921 catcgcatga tcgtcgcgct gcgcggcctg aaggacgcgt cgggtgccgc gatcgaaccg
     1981 tcggcggtgt ttcgcggcta ccgcgatcgg ctcgacacga aggacgtctc cgtcgaagcg
     2041 cgccgcccgc gctacgagca ggtcttcggc gacctggagg acgcaggcgt ggagcgcgac
     2101 gacttgtacg ccgcgtggga cttcacggtt gcgagcaccc agaacctgac cgagcgaatg
     2161 ctcacgattc gcgacgacgc gttcgcggat ctcggcgacg atgcaccttc cttcacgatc
     2221 gatgccgtcg aagagcatcc ggagggcctg ttccggcggg tcacgggcac ggtgcaggtc
     2281 ccgctgtacc tcacgggcgt cggtgatccc ggcagcagct tccactacgg cgacggcgag
     2341 aacgtgccgt cgcgcaacgg caccgagtcg ttcggcgccg gcttcatctg caacattccg
     2401 gaagcggcgc gcacgcaacc cgcgcgcctg tcgttgtacg ggcacgggtt gctcgggtcg
     2461 aacgacgagg tgaacgcggg caatgtgcgc gcgtttgctc aggaacacga catcgtcttc
     2521 tgtgcgacga agtggatcgg catgtcggaa gacgacattc cgaacgccgt cgcgatcctc
     2581 caggatctct ccaagttccc gacactggcc gaccgcgcgc agcagggatt cctgaacgcg
     2641 ttgttcctcg gtcggctgat gcagtccgac gcgttcgcga acgacccgaa cctcgcgggg
     2701 ctgctcgaca cgagcgcgct gtactacgac ggcaacagcc agggtgcgat catgggtggc
     2761 gcgctcaccg cggtgtcgca ggacttcacc cgttcggtgc tcggcgtcac ggggatgaac
     2821 tattcgacgt tgctgcagcg cagcatcgac tgggacacct accgggcggt ctacgaccct
     2881 gcgtaccccg acgagatcga gcgcggcatc ggtatctcgc tgatccagat gctctgggac
     2941 cgggccgagg ccaacggcta cgcgaaccac atgaccggcg acccgttgcc cgacacaccc
     3001 gagcacaccg tgctcttgca cgtcgcgctc ggcgatcacc aggtcgcgaa cgtcgcggcc
     3061 gaggtcgagg cgcgcaccat cggcgcgcgc atccactgcc cggcgaccac cgacggccgg
     3121 ctgcccgacg tcgagcccca ctgggatctc gagtgcgcga aggacggcga caccggttcg
     3181 gtgctcgtgt ggtgggacag tggcacgcca cccgcgccgc cggtcaacct cgcgccgagc
     3241 gggggcgagg atccgcacgg tgatccgcgg tcgagcccgg acgcgcgcac gcagaagtcc
     3301 gagttcctgc agcccgacgg gacgttcgtc gacgtctgca acgggcaggc gtgcaccgcg
     3361 gcaccgtcgt aatcttcccg gcatggccac cgaaacgccg gaccggaccg ggacgcaagc
     3421 gtcgcgcgac acgcgggagt ggagcctcgg caagaagtgg cggtacgtcc tcgccaccac
     3481 gaacccgccg cccggccgcc tcgacccggt ctcgaagtgg ctctacctga cccgggccgg
     3541 tgtgctgccg atgacgctcg tctcggccac cgtcgcgggc ctgctcgcag tgtaccgcga
     3601 cgacaacgtc tcgtggggct ggttcgcgct cgcgtcgctc ggcatcgtgc tggcgcacat
     3661 ggccaacaac ttgatgaacg atctcttcga cgtgaaggtc ggcaccgaca ccgaggacta
     3721 cccgcgcaac ctctattcgc cgcaccccgt gctgtcgggt gtgatctcga agaagggttt
     3781 ggcgacgtgc gcgttggtcg tcaacgcgat gtgcttcgtc atcatgatcg tgctgacggt
     3841 cgcgcgcggc tgggccatcg tcgcgttcgc gctcggcggc tacatcctct ccgcctcgta
     3901 caccgcaccg ccgctgcggt tgaagaagcg cggcctcggt gaaccgacgg tgctgatcgt
     3961 gtggggtccg ctcatggtcg gtggcgtcta ttacgcggcg accggcagca tccccggcgc
     4021 ggtcatcctc gcgtcgttgc cgtacgcgtt gttgtgcacg accgtgttga tgggcaagca
     4081 catcgacaag ctgccgtggg acgaacgtga cggcacccgc acgctgccgg tgatgctcgg
     4141 cgaagcgcgt gcccgtgcga tcaccaaggg catgttcgcg gtgttctacg cgtcggtggt
     4201 cgcgctcgtg atcgcgcaga tcctgccgat cgcgtcgctc ctcgtgttcc tgtcggcgcc
     4261 gatcatggtg cgcacgtgga aggcgtacgg cgaacccaag cccgcggagt cacccatgcc
     4321 gaacccggtg tggccgctgt ggttcgcgcc gcactcattc ctcgtcaccc gccgcgccgg
     4381 cagcctcttg gtcctcggcc tcctgatcgg cgccatcgtc ggctggtaac gcccgagtga
     4441 tctctgcgag aaacacgcgg acggcgtcgc cgtcgatgct gcggcggaac ggcggcatcg
     4501 tcgccaggat cggccggcgg tagcccgcgg tcgcgaggcg gcggtcgagc accgcgacga
     4561 caccgcgatc ttcgcgatga cggatgagac gacccgcgcc ctgcgccagc agcatcgcgg
     4621 cgcgcggcac gtcgacggtc gcgaacgggt cgaggccggc ttgcaccgcg cgctcgcgcc
     4681 gcgccgcgtg caacggctcg ttcgggcggg ggaacgggat gcggtcgatg gtgaccaggc
     4741 tcagcccgac acccgcgacg tcgacacccg tccagaaccc catcgtcgcg aacaccgcga
     4801 cgccaccggc ggtcgcgttg tcgcgcaacg agtcgacgag cacccggcgc ggccggtcgc
     4861 cctgccgcag cacttcgtac tcgccctttg ccgcgcagcg gtcggccgcg gcgcgcatcg
     4921 cgttccatga cgtgaacagc gcgagcgtgc gcccacccgc cgcgacgatc aacgagtgca
     4981 gttcgtcggc gagcgcgtcg gccgcgccgg gcgcacgcgg gtcggggaga tgtttcggga
     5041 cgtagagcat cgcctgttcg cggaagtcga acgggctcgg cacgcggtcg ccgtcccagt
     5101 tgtcgtcggc ggtgaggccg aagcgcgccg ccatcgcgtc gaagttcccg ccgaccgcca
     5161 gcgtcgccga cgtcgcgacg acagtcgcgg tggggagcaa ctgctgcgac aggacatcgc
     5221 cgacgacgac gggcatgagc gcgagccgtg gcgtcgaccc ttcggtcgtg caccacgcga
     5281 cgtgggtgtc gtagacgtcg gggtcgaggg cgcgcccgac ttcgtacgcg agcgtctgcg
     5341 cggccttctc ggcgcgggtc ttgcgggccg acgccggcga gcccgcgtcg agtttggcgt
     5401 ccttcaactt cttcagcgcg tcggcgacgg ccgccgcaat cgtcgcgagg atctccgcca
     5461 gcggcccttc gcacgcgtgc acccgggggt cttcgggtac ggccgccgcg gtgcacagct
     5521 cgtcgaggcg cttgcccgcg cggcgcaagc ggtcgacggc ctcgtgcgcg ttcgcgacgg
     5581 gctcgacgag cgcggcgagg tgccggagcc ggccgctgtg cacctcgacg ccgaactggt
     5641 ccgcgaagac gtcttcgctg aggtgcgctt cgtcgatcac gaccgcgtcg tgcggcggca
     5701 gcacgtgccc accggccatg atgttcgacc cgtacagcgc ggtgttcacc acgacgatct
     5761 cggacagctg cgtgcgcaca cgcgccgctt cggcctggca gcgcccgccg gccgggcacg
     5821 tcgtggtgcc cggacagtcg cgtg
//
LOCUS       DASUGA010000205         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_866733, whole genome shotgun sequence.
ACCESSION   DASUGA010000205 DASUGA010000000
VERSION     DASUGA010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866733"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..916
                     /locus_tag="VFX21_04040"
     CDS             119..916
                     /locus_tag="VFX21_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016907352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX5095154.1"
                     /translation="MLAARAEAVGFAALLVADHPGSSASPFVALGAAASVTSTIALGT
                     YVVNAGVRDPVHVASDVATLDVVSGGRALLGLGAGHTPAEWEMSGLRYPSAGERVDHL
                     FRFVERVRARLQDPEVLPRPVQPRIPLLLGGGNRRLLAYAVAHADIVALSGLGRTLAD
                     GHTHEVRWRAADVDALAASVRGAACVDVLVQHVEITDEREAVAARFAEDVPSLTVDDL
                     LACPFVLIGTEDEIAAQVRSHHERWGIDRFTVRAPALDAIAAVIARL"
     gene            complement(913..1746)
                     /locus_tag="VFX21_04045"
     CDS             complement(913..1746)
                     /locus_tag="VFX21_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019747291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX5095155.1"
                     /translation="MRAVPVSGALGAEIVDVDVRDLDDAGFEALRKLLHEHEVVFIRG
                     VEFTDDEHIAFAERFGPLRLFPIAKLLGATEPTLQVIADGPDSPPEADDWHTDVTWVA
                     EPPDYAVLRATVVPERGGDTLWASMTAAYEALSAPMRAFLDSLTVRHDNTVFIDAVER
                     KAPDMCEQFDVPNRLRTEYPGVEHPLVRTHPDTGRKSLFLGGDFMREIVALRADESAT
                     LLDFLRRHIEDARYHCRWRWAVGDVAIWDERSTNHRSAGDHFPQHREIRRSEVGGARP
                     R"
BASE COUNT          297 a          669 c          716 g          328 t
ORIGIN      
        1 gcgcggcacg gagatcgaat cgcatcatga acacgagcgg tatgtgagca cggaaccgcg
       61 agaaacgaaa atcgagttcg cgctgcaagc agtcgtgaac gatgcggcgt cgtggacggt
      121 gttggcggcg cgcgcggagg ccgtgggctt cgctgcactg ctcgttgccg accatccggg
      181 ttcgtcggcg tcgccgttcg tcgcgctggg cgcagccgcg tcggtgacat cgacgatcgc
      241 gctcggcacc tatgtcgtga acgcgggcgt gcgtgatccg gtgcacgtcg cgtccgacgt
      301 cgcgacgctc gacgtcgtgt cgggcgggcg cgcactgctc gggctcggcg cgggccacac
      361 accagccgag tgggagatgt cggggctccg ctatccgtcc gcaggcgagc gggtcgatca
      421 cctgtttcgc ttcgtcgagc gcgtgcgggc gcgcttgcag gatcccgagg tgttgccgcg
      481 cccggtgcag ccgcgcatcc cgttgctgct cggcggcggc aatcggcgac tgctcgcata
      541 cgcggtcgcg cacgccgaca tcgtcgccct ctccggactc gggcggaccc tggccgacgg
      601 ccacacgcac gaagtgcgtt ggcgcgccgc cgatgtcgac gccctggcag cgagtgtgcg
      661 gggcgccgca tgtgtcgatg tgctcgtgca acacgtcgag atcaccgacg agcgcgaagc
      721 ggtcgcggcg cggttcgccg aggacgtgcc gtcgttgacc gtcgacgacc tgcttgcgtg
      781 cccgttcgtg ctgatcggca ccgaggacga gatcgccgcg caggtgcgct cacatcacga
      841 gcgctggggt atcgaccggt tcacggtgcg cgccccggcc ctggacgcga tcgcagcggt
      901 gatcgcgcgc ctctagcgcg gacgggcgcc gccgacttcg ctgcggcgga tctcgcggtg
      961 ctgcgggaag tggtcgcccg cgctgcggtg gttcgtcgag cgttcgtccc agatcgcgac
     1021 gtcgccgacg gcccagcgcc aacggcagtg gtagcgcgcg tcctcgatgt gccggcgcag
     1081 gaagtcgagc aacgtcgcgc tctcgtcggc gcgcagtgcg acgatctccc gcatgaaatc
     1141 gccgcccagg aacagcgact tacggcccgt atcgggatgc gtgcgcacca acgggtgctc
     1201 gacacccggg tactccgtgc ggagccggtt cgggacgtcg aactgctcgc acatgtcggg
     1261 tgccttgcgt tcgaccgcgt cgatgaagac cgtgttgtcg tggcgcacgg tgagcgaatc
     1321 gaggaacgcc cgcatcggcg cggacagcgc ttcgtacgcg gcggtcatcg acgcccacaa
     1381 cgtgtcgccg ccccgctcgg gtacgacggt ggcacgcaac accgcatagt cgggcggttc
     1441 cgcgacccag gtgacgtcgg tgtgccagtc gtcggcttcg ggtgggctgt cgggcccgtc
     1501 cgcgatcacc tgcaacgtcg gttcggtcgc acccagcagc ttcgcgatcg ggaagagccg
     1561 caacgggccg aatcgctcgg cgaacgcgat gtgctcgtcg tcggtgaact cgacgccgcg
     1621 gatgaacacg acttcgtgct cgtgcagcaa cttgcgcagc gcctcgaaac cagcgtcgtc
     1681 gaggtcgcgc acgtcgacgt cgacgatctc ggcgccgagc gcacccgaga caggaaccgc
     1741 acgcacgatc gtgaggctag atcgtgagaa cgtggcgagg gaccggcgtc ggtgccggcc
     1801 cctcgtcagt cacatgcgaa cactcggcct cgagctagtc gccaccgttg ttgtggccac
     1861 ccgagttgcc ggggtcgcag tcgttctcgg gcgtcgtttc cgattggttg ccgcgtccgg
     1921 agttgcactt ggccttctcg tgttcgcagt cccagccgaa agccggtgat gcgacaccga
     1981 gagtcaacgc ccccgcgaac agggcaacga
//
LOCUS       DASUGA010000206         2271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_345649, whole genome shotgun sequence.
ACCESSION   DASUGA010000206 DASUGA010000000
VERSION     DASUGA010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345649"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFX21_04050"
     CDS             <1..261
                     /locus_tag="VFX21_04050"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX5095156.1"
                     /translation="RGAGTWRLANHALVVTLEPCPMCAGAAWAARISEIVFGAADPKA
                     GASGSLYNFAADPRLNHESTVTSGVLAEECGALLSDFFAPRR"
     gene            299..383
                     /locus_tag="VFX21_04055"
     tRNA            299..383
                     /locus_tag="VFX21_04055"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:333..335,aa:Ser,seq:cga)
     gene            complement(462..893)
                     /locus_tag="VFX21_04060"
     CDS             complement(462..893)
                     /locus_tag="VFX21_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5095157.1"
                     /translation="MEKIVVGVDGSGHAQAALRWAIAEARVRGASLEVVHAWEIPYLG
                     GTLYGPPFAVETGPFEVEAGALLHHAVTSEDTSGVEVHERVVCEAPTTALLESAKGAA
                     MIVVGTRGRGGFKSLLLGSVSQQVVHYAPCPVVVVPSHDED"
     gene            complement(976..1824)
                     /locus_tag="VFX21_04065"
     CDS             complement(976..1824)
                     /locus_tag="VFX21_04065"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018500083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX5095158.1"
                     /translation="MKIAEIVTRAPVCSFEFFPPKTPALEETLERTLRELEPLGPSFV
                     SVTYGAGGSTRDKTHEIVCRINQETSMTAMAHLTCAAHRRDELEAVVTRYRDAGVENI
                     LALGGDPPKELGLPPGELAHATDLVDLVRAIGDFSVGVAVHPEGHPASTDRDEDRRRQ
                     AEKLALADFGLSQFFFDPQVWFDFLDDLDRLGVTTPVTPGIMPVTNVKSVKRMAEMSG
                     ADFPEWLEARLRAVEDDEQAMYQVGVEAATELCEALRAGGVERFHFYTLNRSPATREI
                     HSNLGL"
     gene            complement(1844..>2271)
                     /locus_tag="VFX21_04070"
     CDS             complement(1844..>2271)
                     /locus_tag="VFX21_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX5095159.1"
                     /translation="TDLDAEALPGDKLALVKQLKDAGHTVAVVGDGVNDAPALAQADL
                     GIAMGTGTDVAIETSDVTLVAGDLRGAADAIALSRRTLATIKGNLFWAFAYNVAAIPL
                     AAFGLLNPVIAAGAMAFSSVFVVANSLRLRRFRSIRDTA"
BASE COUNT          371 a          794 c          746 g          360 t
ORIGIN      
        1 cgcggtgccg gaacgtggcg gctcgcgaat cacgcgctcg tcgtcacact cgaaccctgc
       61 ccgatgtgcg caggcgcggc gtgggcggcg cgcatcagcg agatcgtgtt cggcgccgcc
      121 gatccgaagg ccggcgccag cggcagcctc tacaacttcg ccgccgatcc ccgcttgaac
      181 cacgagtcca ccgtgacctc cggagtgctg gctgaggagt gcggggccct cctcagcgac
      241 ttcttcgcgc cgcggagatg aggatcgggc catgaccgat cccgtatcat ctcgccccgg
      301 agggatgcga gagcggccga atcggatggt ctcgaaaacc attgtggcgc aagtcaccgt
      361 gggttcaaat cccactccct ccgctcatga accccgtgac tccgtcccgg ggttcattcg
      421 cgatatctct ctgtcgctct cgacctactc gcggtgaggc gtcagtcttc gtcgtgggag
      481 ggcacgacga cgaccgggca cggcgcgtag tgcacgacct gctggctcac cgacccgagc
      541 agcaggctct tgaacccgcc gcgaccgcga gtgcccacca cgatcatcgc ggcacccttg
      601 gcgctctcga gcaacgccgt cgtcggcgcc tcgcacacga ccctctcgtg cacctcgaca
      661 ccactcgtgt cctcggacgt caccgcgtgg tgcagcaacg ccccggcctc cacctcgaac
      721 ggaccggttt cgacggcgaa ggggggtccg tacagcgtcc cgccgagata ggggatctcc
      781 cacgcgtgca cgacctcgag tgacgcgccc cgcacgcgag cctcggcgat cgcccatcgc
      841 agcgcggcct gcgcgtggcc cgatccgtcg acaccaacga cgatcttctc catcacgtac
      901 ctcctcgcga cgtcaactct cgtcgccgga ccgagaagtc gccagaggcg aaggtcccca
      961 ggcgcctgta cgcgctcaca acccgaggtt cgagtggatc tctctcgtcg cgggtgagcg
     1021 gttgagcgtg tagaagtgga agcgctcgac gccgcctgcg cgcagcgctt cgcagagctc
     1081 ggtcgcggcc tcgacgccga cttggtacat cgcctgctcg tcgtcctcca ctgcgcgcag
     1141 acgcgcttcc agccactcgg ggaagtccgc acccgacatc tccgccatgc gcttcacgga
     1201 cttcacgttg gtgaccggca tgatccccgg cgtcaccggc gtcgtgacac cgagccggtc
     1261 gaggtcgtcg aggaagtcga accacacctg cggatcgaag aagaactgcg acaggccgaa
     1321 gtcggcgagt gcgagcttct cggcctgccg gcgccggtct tcgtcgcggt cggtcgacgc
     1381 gggatgcccc tcggggtgca cggcaacgcc caccgagaag tcgccgatcg cgcgcacgag
     1441 gtcgacgagg tcggtggcgt gcgcaagctc acccggcggg aggccaagct ctttcggcgg
     1501 atcgccaccc agcgcgagga tgttctcgac gccggcatcg cgatagcgcg tgacaacggc
     1561 ctcgagctcg tcacgacgat gcgccgcgca cgtgaggtgc gccattgccg tcatcgacgt
     1621 ctcttgattg atgcggcaga cgatctcgtg cgtcttgtcg cgcgtcgatc cgcccgcgcc
     1681 gtaggtcacc gacacgaacg acggaccgag cggttcgagc tcgcgcagcg tccgttcgag
     1741 tgtctcctcc agcgcaggcg tcttgggcgg gaagaactcg aacgagcaga caggagcgcg
     1801 ggtgacgatc tcggcgatct tcatcggctg cgcagaatag tgctcacgcg gtgtcgcgaa
     1861 tcgagcggaa gcgccggagc cgcaacgagt tggcgacgac gaacaccgac gagaacgcca
     1921 ttgcgcccgc cgcgatcacc ggattcaaca gaccgaatgc cgcgagcggg atcgcggcga
     1981 cgttgtacgc gaacgcccag aacagattgc ccttgatcgt cgcgagggtg cgtcgcgaca
     2041 gcgcgattgc gtcggccgcg ccgcgcaggt cgcccgcgac gagcgtgacg tcactggtct
     2101 cgatggccac gtccgtaccg gttcccatgg cgatgccgag atcggcctgc gccagcgcgg
     2161 gtgcgtcgtt cacaccgtcg ccgacgaccg cgacggtgtg gccggcgtcc ttgagttgct
     2221 tgacgagcgc gagcttgtcg ccgggcaggg cctcggcgtc gaggtcggtg a
//
LOCUS       DASUGA010000207         5182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1838478, whole genome shotgun sequence.
ACCESSION   DASUGA010000207 DASUGA010000000
VERSION     DASUGA010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1838478"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VFX21_04075"
     CDS             complement(<1..432)
                     /locus_tag="VFX21_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEX5095160.1"
                     /translation="MAPVLSLSDVTFGYGATPVLDGVTLAVEPGEFAALVGPNGSGKS
                     TLLRIVLGVLRPQHGTVRLLGADPADLHDRARIGYVPQRALLSPDIPATVEEVVRAGR
                     LARRGWWRWPGPADRTAVDHALQSVALTDMRERRVRELSGGQ"
     gene            complement(480..1421)
                     /gene="map"
                     /locus_tag="VFX21_04080"
     CDS             complement(480..1421)
                     /gene="map"
                     /locus_tag="VFX21_04080"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013937267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX5095161.1"
                     /translation="MSKRNQKERLARRRAASVRRPGAEGARRLVKPGRLSPDRTVPAS
                     IPRPPYAPDAPALTPAVPPDEFAERMRAAGRAAREVLLEIEAAVAVGVTTDELDRVCH
                     EACIARGGYPSPLHYKGYPKSLCTSVNEIICHGIPDDRKLVDGDIVDCDVTIFLNGVH
                     GDCNATYLVGDVDEIGRDLVRVTRESMWAGIDAVKPGAHVYDIGRAIQAHAEGNGFGV
                     VRAFVGHGIGEIFHTAPSVPHFFDKHADTLLVPGMTFTIEPMITEGSWELGRIWADGW
                     TAPTKDLSRCAQFEHTVMVTGTGVDVLTLLPREPHGT"
     gene            complement(1418..1816)
                     /locus_tag="VFX21_04085"
     CDS             complement(1418..1816)
                     /locus_tag="VFX21_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HEX5095162.1"
                     /translation="MSDQTVFEAVGGQAFFDRLVDAFYERVEHDEVLRPLYPDDLEPG
                     KHALALFLGQYWGGPPAYSAVKGHPRLRMRHAPFVIGPAERDAWLTAMFAALTDAAPP
                     PEIETAMRDYFEMAATHMINSVPPGATMGS"
     gene            1875..2381
                     /locus_tag="VFX21_04090"
     CDS             1875..2381
                     /locus_tag="VFX21_04090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HEX5095163.1"
                     /translation="MHQSWLNPLAEVRSAGAKGMGCFARQDLPAGTTVAGFGGHVVER
                     AEFDELDEELRTHALQIDDRLYMVSLPPFAPADYVNHSCEPNCGIVGSCLLVTRRDVS
                     VGEELCFDYAMSDTNDYDEFVCECGTGCCRRLITGGDWKLPELQARYAGFFSSYITRR
                     IAQTAPTE"
     gene            complement(2389..3078)
                     /locus_tag="VFX21_04095"
     CDS             complement(2389..3078)
                     /locus_tag="VFX21_04095"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX5095164.1"
                     /translation="MSPSPKRAPAKKKLPIVPIVIGVIAVAAVAAIAFTMLGSDDKQD
                     ATKDGESAPVTVTGDTLPPFPNSQPNDNPGGAAPVLQGKNFAGEDVTIPADDGKAKVI
                     FFVAHWCPHCQREIPNLAEWLQSNELPDNVEIAFVSTRVLEGGTTANYPPSKWLADNG
                     FGDYPTIADTKDDNPAYYAYGAGGLPYVVYLDADNNVVLRTAGELGDSSVYTDIFDAL
                     SKGETPKDPRE"
     gene            complement(3075..3680)
                     /locus_tag="VFX21_04100"
     CDS             complement(3075..3680)
                     /locus_tag="VFX21_04100"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEX5095165.1"
                     /translation="MLDSNPWINFFVALTVLANVATLTLWVLGIASALGFGRALWQQV
                     RDALGDTGITLAAIVAGTAMVGSLYMSEGAHFLPCKLCWYQRIGMYSLAIILVIAAVR
                     RDWNIRIYATTLAVLGGIVSTYHYLIERFPSLEKGTSCDPTNPCTFVWFFHLHYISIP
                     FMALSAFALVATMLSVARPAPTEDDGERVLDDALRQEESPV"
     gene            complement(3712..4158)
                     /locus_tag="VFX21_04105"
     CDS             complement(3712..4158)
                     /locus_tag="VFX21_04105"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX5095166.1"
                     /translation="MRGATDELRETVGRRLGRHQLRLTSVREHIVDVLASSDRPLTIP
                     EILASRDGLAQSSVYRNLVVLEDADVVRRVVTHDEFARYELAEGLTEHHHHLVCSNCG
                     RVEDLPASAALERSVQSAVELAASKAGFRTEHHRLDLVGTCADCAS"
     gene            4206..4994
                     /locus_tag="VFX21_04110"
     CDS             4206..4994
                     /locus_tag="VFX21_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX5095167.1"
                     /translation="MQDPAERFANVVAPDRAAVHLDEAALCIAAHAREGLDVDEWCSR
                     LDDLAGEARLPSFDEVRHVLFEEHDFRGDTAHYQDPENSFLDAVLTRERGIPISLSVV
                     VIEVARRLGIPVHGVGMPGHFLVQDADSDGFWLDAFHGGALLQYEDCARIVDRIYRGT
                     RRLVPSDLTPTPARAILARMLANLEGGSLASDPTQLAWMLRLHVMIPGTAVHARLQLL
                     RQFAEVGHPAHVAQAYDLVVGQLDDDKAREQVDAEATRYRRRWN"
     gene            5012..>5182
                     /locus_tag="VFX21_04115"
     CDS             5012..>5182
                     /locus_tag="VFX21_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005169325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX5095168.1"
                     /translation="MFPLGTVLFPHALLPLQVFEPRYRVMMRNLLDGEREFGVVLIER
                     GSEVGGGDVRFDV"
BASE COUNT          808 a         1758 c         1774 g          842 t
ORIGIN      
        1 ctgtccgccc gagagctcgc ggacgcgtcg ctcgcgcatg tcagtgagcg cgaccgattg
       61 cagcgcgtgg tcgactgcag tgcgatcagc cggaccgggc caccgccacc agccgcgccg
      121 cgcgaggcgg ccggcgcgca cgacctcttc gaccgtcgcc gggatgtcgg gcgacaacag
      181 cgcgcgctgc ggcacgtacc cgatccgagc gcggtcgtga agatccgcgg gatccgcgcc
      241 gagcaaccgc acggtgccgt gctgcggccg caagacgccc aacacgatgc ggagcagcgt
      301 cgacttccca ctgccgttcg gcccgacgag cgccgcgaac tcccccggct cgacggcaag
      361 cgtcacaccg tcgagcacag gcgtagcccc gtagccaaag gtgacgtcgg acagcgagag
      421 gacgggagcc atagaatgag agtgattctc accctagtct ctcgacgggt attccctggt
      481 caggtgccgt gaggttcgcg tgggaggagg gtcaggacgt cgacgccggt gccggtgacc
      541 atcaccgtgt gctcgaactg ggcgcagcgg gaaaggtcct tggtgggcgc ggtccagccg
      601 tcggcccaga tccggcccag ctcccatgag ccctcggtga tcatcggctc gatcgtgaac
      661 gtcatgccgg gtacgagcag cgtgtccgcg tgcttgtcga agaagtgggg caccgacggc
      721 gcggtgtgga agatctcgcc gatgccgtga ccgacgaacg cgcgcacgac gccgaagccg
      781 ttgccctccg cgtgcgcctg gatcgcgcgg ccgatgtcgt agacgtgcgc gcccggcttc
      841 accgcgtcga tgccggccca catcgactcc cgcgtcacgc gcacgagatc gcggccgatc
      901 tcgtcgacgt cgcccacgag atacgtcgcg ttgcagtcgc cgtgaacacc gttcaggaag
      961 atcgtgacgt cgcagtcgac gatgtcgccg tcgacgagct tgcggtcgtc ggggatgccg
     1021 tgacagatga tctcgttcac cgacgtgcac aacgacttcg ggtagccctt gtagtgcaac
     1081 gggctcgggt agccgccgcg cgcgatgcat gcctcgtggc acacgcggtc gagctcgtcg
     1141 gtagtgaccc cgaccgcaac cgcggcttcg atctcgagca gcacctcacg cgcggcccgt
     1201 cccgcggcgc gcatgcgttc ggcgaactcg tcgggcggca ccgcgggggt cagtgcgggc
     1261 gcatcggggg catagggcgg gcgcgggatc gaggccggca ccgtgcgatc cggcgacagc
     1321 cggcccggct tcacgaggcg ccgggcccct tctgcgcccg ggcggcgcac cgatgcggcc
     1381 cgccggcgcg cgagacgttc cttctggttc cgcttgctca cgatcccatt gtcgcgccgg
     1441 gaggcacgct gttgatcatg tgggtcgcgg ccatctcgaa gtagtcgcgc atcgccgtct
     1501 cgatctcggg tggcggggcg gcgtcggtca gcgccgcgaa catcgcggtc agccacgcgt
     1561 cccgctcggc cggaccgatg acgaacggcg catgccgcat ccgcagccgc gggtgaccct
     1621 tgaccgcgct gtacgcgggc ggaccacccc agtactgccc gaggaacagg gcgagcgcgt
     1681 gcttccccgg ttcgaggtcg tcgggataca gcggacggag cacttcgtcg tgctcgaccc
     1741 gctcgtagaa cgcgtcgacc aggcgatcga agaacgcttg tccaccgacc gcctcgaaaa
     1801 cggtttggtc actcacccgg tgtagtgtcc tcgattcctc tgccggatct cgaacgcgcg
     1861 caaagcgagg tgacgtgcac cagtcctggc tcaatccact cgccgaagtc cgttccgccg
     1921 gcgccaaggg aatggggtgc ttcgcccgcc aagacctgcc cgcgggcacg acggtcgccg
     1981 gcttcggcgg tcacgtcgtc gagcgcgccg agttcgacga gctcgacgaa gagctgcgca
     2041 cgcacgcgtt gcagatcgac gatcgcctct acatggtgag cctgccgccg tttgcgccgg
     2101 ccgactacgt caaccactcc tgcgagccca actgcgggat cgtcgggtcg tgcttgctgg
     2161 tgacgcgccg cgacgtgtcg gtcggcgaag agctgtgctt cgactacgcg atgtcggaca
     2221 ccaacgacta cgacgagttc gtctgcgagt gcggcaccgg ctgctgccgg cgcctgatca
     2281 ccggcggcga ctggaagctc cccgagctcc aggcgcgcta cgccggcttc ttctcctcgt
     2341 acatcacccg ccggatcgcg caaaccgcac cgacggaata gcgactcgct attcgcgcgg
     2401 gtcctttggc gtttcgccct tcgacagcgc gtcgaagatg tcggtgtaga cgctcgagtc
     2461 gccgagctca cccgccgtcc gcaacaccac gttgttgtcc gcgtcgaggt agacgacgta
     2521 cggaaggccg ccggcgccgt acgcgtagta cgcggggttg tcgtccttcg tgtcggcgat
     2581 cgtcgggtag tcgccgaagc cgttgtcggc gagccacttc gacggcgggt agttcgcggt
     2641 cgtcccgccc tccaggacgc gcgtcgacac gaacgcgatc tcgacgttgt cgggcagctc
     2701 gttgctctgc agccactccg ccaggttcgg gatctcgcgc tggcagtgcg ggcaccagtg
     2761 ggcgacgaag aagatcacct tcgccttgcc gtcgtcggcg gggatcgtga cgtcttcgcc
     2821 tgcgaagttc ttgccctgca agacgggcgc ggcgcctccc gggttgtcgt tgggttgact
     2881 gttcgggaag ggcggcagtg tgtcgccggt caccgtgacc ggcgcggact cgccgtcctt
     2941 cgtcgcatcc tgcttgtcgt cgctcccgag catcgtgaag gcgatcgcgg cgacggccgc
     3001 gacggcgatc acgccgatca cgatcggcac gatcggcagc ttcttcttcg ccggcgccct
     3061 cttcggtgac gggctcatac gggggactcc tcctgtcgga gcgcgtcgtc gaggacacgc
     3121 tcaccgtcgt cttccgtcgg cgcgggccgg gccaccgaca acatggtggc gacgagcgcg
     3181 aacgccgaca acgccatgaa cgggatcgag atgtagtgca ggtggaagaa ccacacgaac
     3241 gtgcacgggt tcgtcgggtc gcaggacgtt cccttctcga ggctcgggaa ccgctcgatc
     3301 aggtagtggt acgtcgagac gatgccgccg agcacggcga gcgtggtcgc gtagatgcgg
     3361 atgttccagt cgcgccgcac ggctgcgatg acgaggatga tcgcgagcga gtacatgccg
     3421 atgcgctggt accagcacag cttgcacggc aagaagtgcg caccttccga catgtacagc
     3481 gaaccgacca tcgcggtgcc cgcgacgatc gcggcgagcg tgatgcccgt gtcaccgagt
     3541 gcgtcgcgca cctgctgcca gagcgctcga ccgaagccga gcgcggacgc gatgccgagc
     3601 acccacaacg tgagcgtcgc gacgttcgcg agcaccgtga gggcgacgaa gaagttgatc
     3661 caaggattgc tgtccagcaa ctcgcccgcc cgccctaacc ctgcatgtcg ttcacgaagc
     3721 acaatcggca cacgtgccga ccagatcaag acgatgatgc tccgtgcgga accctgcttt
     3781 gctcgcggcc agctccaccg ccgattggac cgaccgttcg agcgccgccg atgccgggag
     3841 gtcctcgacc cgaccgcagt tcgagcacac caggtggtgg tgatgctcgg tcaatccctc
     3901 ggccagctcg tagcgcgcga actcgtcgtg cgtgaccacc cggcgcacga cgtcggcgtc
     3961 ctcgagcacg acgagattgc ggtacaccga gctctgcgcg aggccgtcgc gggaagccag
     4021 gatctcggga atcgtcagcg ggcgatccga tgacgcgagc acgtcgacga tgtgctcgcg
     4081 cactgatgtg aggcggagct gatgtcgacc gagccggcgt ccgacggtct cgcgcagctc
     4141 gtcggtggcg ccacgcacgc cgccgagtct accggccgat cggggcctct cgtacgctcc
     4201 ggggcgtgca ggatccggcg gaacggttcg cgaacgtcgt tgcgccggac cgggcggcgg
     4261 tccatctcga cgaggcggcg ctgtgcatcg ccgcgcacgc gcgcgaggga ctcgacgtcg
     4321 acgagtggtg cagccggctc gacgacctcg cgggtgaggc gcggctgccg tcgttcgacg
     4381 aagtccggca cgtgctgttc gaagagcacg acttccgcgg tgacacggcg cattaccagg
     4441 atccggagaa ctcgttcctc gacgccgtgt tgacgcgcga gcgcggcatt ccgatctcgt
     4501 tgtcggtggt cgtcatcgag gttgcgcgcc ggctcggtat tcctgtgcac ggcgtcggca
     4561 tgcccggcca cttcctcgtg caagacgccg acagcgacgg cttctggctc gatgcgttcc
     4621 atggtggcgc gctgttgcag tacgaggact gcgcacggat cgtcgaccgc atctaccgcg
     4681 gtacccgacg gctcgtgccg tccgacctca cgccgacgcc ggcccgcgcc atcctggcgc
     4741 gcatgctcgc gaatctcgaa ggaggctcgc tcgcgtccga cccgacgcaa ctggcgtgga
     4801 tgctacggct ccacgtgatg atcccgggca ccgcggtgca cgcacgcctt cagctgctgc
     4861 gccagttcgc ggaggtcggg cacccggcgc atgtcgcgca ggcctacgac ctcgtcgtcg
     4921 gtcagctcga cgacgacaag gcgcgcgaac aagtcgacgc cgaggcaacg cgctaccgcc
     4981 ggcggtggaa ttgacgcgcg acgcgctccc gatgttcccg ctcggcacag tgctgttccc
     5041 gcacgcgctg ttgcccttgc aggtgttcga gccccgttac cgcgtgatga tgcggaactt
     5101 gctcgacgga gagcgggagt tcggcgtcgt gctcatcgaa cgggggagtg aggtcggtgg
     5161 cggcgacgtt cgtttcgacg tc
//
LOCUS       DASUGA010000208         3605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1717653, whole genome shotgun sequence.
ACCESSION   DASUGA010000208 DASUGA010000000
VERSION     DASUGA010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1717653"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFX21_04120"
     CDS             complement(<1..185)
                     /locus_tag="VFX21_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095169.1"
                     /translation="MRSLRRAFVLLIAIIGVVAVTGCAGAPPILPSSSPAPSVMAAQD
                     EGAEQALINSINSLRAS"
     gene            complement(415..1512)
                     /locus_tag="VFX21_04125"
     CDS             complement(415..1512)
                     /locus_tag="VFX21_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX5095170.1"
                     /translation="MADDPKIELDTPWRRDPHELASGLEQWARHTIDPALTVRDVNAP
                     GNGMSSESVLFTLAGGAAAGDYVARLAPMPAVYPVFPEYDLDVQRRCMQLVRARTDVP
                     APEVPYGEPDASWLGTPFIVMKRVDGVPPLDVPPYVMDGWLLEATDEQRAAVQRHAAS
                     VLARLHTITPDNADLSFLMRPEHGATALEQHLGYQRWYYDWAREGETYPLIEQTFAWL
                     AAHMPTGHETVLNWGDARLGNMLWANFEPVAVLDWEMAAVGPPEIDLAWMIFLHAFFQ
                     DLAAKFEIVGLPEFMRRENVVADYESCSGRTVRNLDWFEVFAALRFAIVSVRTSTRGI
                     AYGSMEKPDDVDDLIMFRGMLERMLDSTYWT"
     gene            1580..2629
                     /locus_tag="VFX21_04130"
     CDS             1580..2629
                     /locus_tag="VFX21_04130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5095171.1"
                     /translation="MGGFNVISPAPRHRWRELLGEDPDALPTQAPEGIDAMCARSLWR
                     DASRLYTTDAGRSFVLPMVRLGPMGPGAIEASPRRGWGYGGVIAPGGVTLADLDIVAR
                     DLAAHRVLRRTIRPNPIHAEVWRSFSGMQRVASDAHVIDLGPGLDVVYKGFNRNARRN
                     INMAEQAGVRVEVDRTGRLLAEFFELYDLSRARWAHQQHEPQWLARIRYGRQDPAKKW
                     RQISSHLGSRCAVMIARVGTRPAAGGIMLFGQNAHFTRAAMDLEIAGPTRATDALEWE
                     SIKEACASGARWFQLGHSSSPGVAAFKERFGAVRVDYEEFVSERLPITRVDKMLRSAV
                     KQIVRFDEDATGPPA"
     gene            2656..3066
                     /locus_tag="VFX21_04135"
     CDS             2656..3066
                     /locus_tag="VFX21_04135"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX5095172.1"
                     /translation="MSENLRFLGKYSAAMESGDTDAVYEFFSPDFHSHVSDRTGFTRG
                     TDARLGEQQWWDNVRSAFPDMQFSVDLLIEQGELVVSNWTVRGTHTGTAFYDVPASGN
                     PVIINGTAILRIVDNQIVEHWGGPHCQEGLGLIK"
     gene            3177..>3605
                     /locus_tag="VFX21_04140"
     CDS             3177..>3605
                     /locus_tag="VFX21_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095173.1"
                     /translation="MGLLWYKVVSPAPRARWREVLLQDPDALPSHSPEWTDAMCANSR
                     WRDASRLYITNEGRSFVMPMVRFGPLRRFLAIEASPRPGWGFGGIVAPGGVTSADIAV
                     VADDLAHRRPMRREIKPNPVQVDLWRGFTGMRRVPTEAHVI"
BASE COUNT          596 a         1140 c         1241 g          628 t
ORIGIN      
        1 ttcgacgcgc gcagcgagtt gatcgaattg atgagcgctt gctctgcgcc ttcgtcctgc
       61 gcggccatga ccgatggtgc gggactactc gacggaagaa ttggtggtgc acctgcacaa
      121 ccggtaacgg cgacgacacc gatgattgcg atcaacaata cgaatgcccg gcgaaggctg
      181 cgcatagaga gagcgtctcc tggttggcga catgaacatg cgccgatcca tgcgcaagag
      241 aggttcatcc gtgcgtgcgg aagttgcccg cgccggacga taacgaaacg gttctgcagt
      301 ttcgtggtta tgcgcgcgag ttctctttag cgtttgggat tcacgcggca tcgcggttga
      361 cgcgttgccc gattacgcgc ggtcagtgac cggtcagaac ggacgctcgg tgcgctatgt
      421 ccaatacgtg ctgtcaagca tccgttccag catgccgcgg aacatgatga gatcgtcgac
      481 gtcgtcgggc ttttccatcg agccgtacgc aataccgcgt gtgctcgtgc gcacggaaac
      541 gatcgcgaac cgcaacgcgg cgaagacttc gaaccaatcg aggttgcgca ccgtgcgacc
      601 ggaacacgat tcgtagtcgg cgacgacgtt ctcgcggcgc atgaactccg ggagtccgac
      661 gatctcgaac ttggccgcga ggtcctgaaa gaacgcatgc aggaagatca tccacgcgag
      721 gtcgatctcg ggtggtccga cggccgccat ctcccagtcg agcaccgcga ccggttcgaa
      781 gtttgcccac agcatgttgc cgagccgcgc gtcgccccaa tttaggactg tctcgtggcc
      841 ggtcggcatg tgcgcggcga gccacgcgaa cgtctgctcg atgaggggat aggtctcgcc
      901 ctcgcgtgcc cagtcgtagt accaacgctg atagccgagg tgttgttcga gcgcggtcgc
      961 gccgtgctcg ggacgcatca agaacgacag atccgcgttg tcgggcgtga tcgtgtgcag
     1021 tcgcgcgagc acgcttgccg cgtgacgttg cacggccgcg cgttgttcgt cagttgcttc
     1081 gagcaaccaa ccgtccatga cgtacggcgg tacgtcgagg ggcgggactc cgtcgacgcg
     1141 cttcatgacg atgaacggcg tgccgagcca actcgcgtcg ggttcgccat agggaacctc
     1201 gggcgcggga acgtcggtcc gggcgcgcac gagttgcatg cagcgacgct gcacgtcgag
     1261 gtcgtactcg gggaagacgg gatagaccgc cggcatcggc gccagccggg ccacgtagtc
     1321 gccggccgcg gcgccacccg ccagggtgaa caggacgctc tcgctcgaca tcccgttgcc
     1381 cggcgcgttc acgtcgcgaa cggtgagggc cgggtcgatc gtgtgccggg cccactgctc
     1441 gaggccggac gcgagctcgt gggggtcgcg ccgccagggc gtgtcgagct cgatcttggg
     1501 gtcgtcagcc atgggacaag gatgccgtac cgggccgttc gggatcgact ctctcgttct
     1561 gggtaagaag ggccaacata tgggcggctt caacgtcatt agcccggcgc cgcggcaccg
     1621 gtggcgagaa ttgctgggcg aggacccgga cgcgctgccg acccaggcgc cggagggaat
     1681 cgacgcgatg tgcgcccgtt cgttgtggcg tgacgcaagc cggctgtaca ccaccgacgc
     1741 gggccgttcg ttcgtgctgc cgatggtgcg gctcgggccc atgggcccgg gtgcgatcga
     1801 agcgtccccg cgccgcggtt ggggctacgg cggcgtgatc gcgcccggcg gtgtgacgct
     1861 ggccgatctc gacatcgtcg cgcgcgacct cgccgcgcat cgcgtgttgc gccgcaccat
     1921 ccgacccaac ccgatacatg ccgaagtgtg gcgcagcttc agtggcatgc aacgagtcgc
     1981 gtcggacgcg cacgtgatcg atctcggtcc ggggctcgac gtggtctaca agggcttcaa
     2041 tcgcaacgcg cgccgcaaca tcaacatggc cgagcaggcc ggagtcaggg tcgaggtcga
     2101 tcgcaccggc cgtttgctcg cggagttctt cgagctctac gacctgtcac gggcgcggtg
     2161 ggcgcaccag cagcacgaac cgcagtggtt ggcgaggatc cggtacggcc gtcaggaccc
     2221 ggcgaagaag tggcgacaga tcagcagcca tctcggctcg cggtgcgcgg tgatgatcgc
     2281 gcgcgtcggg acccgaccgg cggccggcgg catcatgttg ttcggccaga acgcacactt
     2341 cacgcgcgcg gcgatggacc tcgagatcgc aggtcccaca cgcgcgaccg acgcgctcga
     2401 gtgggagtcg atcaaggaag cgtgcgccag cggtgcacgg tggttccagt tgggtcacag
     2461 ttcgtcaccg ggcgtcgccg cgttcaagga acgcttcggc gcggtgcgcg tcgactacga
     2521 ggagttcgtg tccgagcgac tgcccatcac ccgcgtcgac aagatgttgc ggtcggccgt
     2581 gaagcagatc gtccgcttcg acgaggacgc gaccggccct cccgcgtagg ggcgcagcgg
     2641 tgctaccgtc cggccatgtc ggagaacctg cgcttcctgg gcaagtacag cgcggcgatg
     2701 gagtccggtg acaccgacgc ggtgtacgag ttcttctcgc ccgacttcca cagccatgtc
     2761 agcgaccgca ctggtttcac gcgcggcacg gacgcgcgtc tcggcgaaca gcagtggtgg
     2821 gacaacgtgc gcagcgcgtt ccccgacatg cagttctccg tcgatctgct gatcgaacag
     2881 ggcgagctgg tcgtgtcgaa ctggaccgtg cgcggcaccc acaccgggac cgcgttctac
     2941 gacgtcccgg cgtcgggcaa tccggtgatc atcaacggca ctgcgatctt gcgcatcgtc
     3001 gacaaccaga tcgtcgagca ctggggtggc ccgcactgcc aggaggggct cggcctcatc
     3061 aagtaagtcg ccgtgctcgc cgggtcgcgg tcgttcgcgg cttttccgcg gtgcaacgtg
     3121 acgcgcgtca ccaaacctcg ccaaacctcg tcgtcgcgtg cattgtgtcc cgcaacatgg
     3181 gtttgctctg gtacaaggtc gtgagcccgg cgccgcgcgc tcgttggcgt gaagtgctcc
     3241 tgcaggaccc ggacgcgttg ccgtcgcatt cgcccgagtg gaccgatgcg atgtgtgcga
     3301 actcgcgctg gcgtgatgcg agccggttgt acatcacgaa cgagggacgt tcgttcgtga
     3361 tgccgatggt tcgcttcgga ccactccggc gcttcttggc catcgaggcg tcgccgcggc
     3421 cgggttgggg cttcggcggg atcgtcgcgc ccggcggtgt gacgagcgcg gacatcgccg
     3481 tcgtcgccga cgatctcgcg caccgccgcc cgatgcggcg agagatcaaa ccgaacccgg
     3541 tgcaagtcga tctgtggcgt ggctttaccg gcatgcgtcg tgtgcccacc gaggcgcacg
     3601 tcatc
//
LOCUS       DASUGA010000209         2317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1561114, whole genome shotgun sequence.
ACCESSION   DASUGA010000209 DASUGA010000000
VERSION     DASUGA010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561114"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VFX21_04145"
     CDS             <1..770
                     /locus_tag="VFX21_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007529897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5095174.1"
                     /translation="GTAIVPAYTRGAHTLASQVASACQAMPGKFALGIGTSSNVIVER
                     WNDIAFEKPYQRVRDTIAFLRTALTGEKVDEEYETFKVRGFRLGIKVPEQPPILVAAL
                     RQGMLKLAGTKGDGAIINWLSADDVKTVVPHVGGKEVVARIFVLPVDDAGMARAVGKR
                     AIASYLTVPVYAAFHEWLGRGDELADLWRLWKEGDRKAAAESIPDSVVDDLLVWGSPQ
                     ACKEHIARYMENGVTTPAPALFVGPDQLRDVVRALAR"
     gene            complement(767..1522)
                     /locus_tag="VFX21_04150"
     CDS             complement(767..1522)
                     /locus_tag="VFX21_04150"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX5095175.1"
                     /translation="MRTAQWIAVVAFALLVTQIATAATTVYLHRGLSHRALTVKPAAA
                     MPFRFFIWITTGMKPREWVAVHRKHHAATDTPEDPHSPKVLGFWRVQLGNVGLYKKVA
                     SDELQVHKYARDIKRDKWDRLFFDHALFGLGVGIGLLVTVTWGLGFGIVVGLLAAGLH
                     AVSFVMMSGAINAVTHTYGKQPYDNSATNLQSLALVTGGEALHNNHHAAPTSAKFALA
                     KGQIDPGWYLVKVLVFFKLASVRHEEVHLKSAA"
     gene            1570..2190
                     /locus_tag="VFX21_04155"
     CDS             1570..2190
                     /locus_tag="VFX21_04155"
                     /inference="COORDINATES: protein motif:HMM:NF019961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5095176.1"
                     /translation="MAIAANSDPTTKDLILAAATRRFADHGYAGTSLNDIADEVGIRR
                     PSLLHHYPSKEVLYRAVLYSSFEDWSGLVDEAIVGTREGWPQVERMLRAAFTFFEDHP
                     DFVRLVRWEALEGGPRISHELSLLLKPLFDRGAAFLEREMDAGRLKRYDARQLLLTGY
                     GAVLSYLSDGQLMSGLLDTDPLSPEMLAVRREHVIAVLRTAVAPTS"
BASE COUNT          395 a          763 c          763 g          396 t
ORIGIN      
        1 tgggtacggc gatcgtgcct gcgtacacgc ggggcgcgca cacgctcgcg tcgcaggtcg
       61 cgtcggcgtg ccaagcgatg ccgggcaagt tcgcgctggg catcggcacc tcgtcgaacg
      121 tgatcgtcga gcgctggaac gacatcgcct tcgagaagcc gtaccagcgc gtccgcgaca
      181 cgattgcgtt cctgcgcacc gcgctcaccg gcgagaaggt cgacgaggaa tacgaaacct
      241 tcaaagtgcg cggctttcgg ctcggcatca aggtgcccga gcagccgccg attctcgtcg
      301 ccgcgctccg ccaagggatg ctgaagctcg cgggcacgaa gggtgacggc gcgatcatca
      361 actggttgtc ggccgacgac gtcaagaccg tcgttccgca tgtcggcggc aaggaagtcg
      421 tcgcgcgcat cttcgtcctc ccggtcgacg acgccgggat ggcgcgcgcg gtcggtaagc
      481 gcgcgatcgc gagctacctc accgttccgg tgtacgccgc gttccacgag tggctcgggc
      541 gcggcgacga gctcgcggat ctctggcgct tgtggaagga aggcgaccgc aaggccgcgg
      601 ccgagtcgat ccccgactcc gtcgtcgacg acctgctcgt gtggggctca ccgcaagcgt
      661 gcaaggaaca catcgcgcgc tacatggaga acggcgtcac cacaccggca cccgcattgt
      721 tcgtcggccc cgaccagttg cgcgacgtcg tccgagcgct cgcacgctaa gccgcggatt
      781 tgagatgcac ttcttcgtgg cgcactgacg ccagtttgaa gaagacgagc accttcacga
      841 ggtaccagcc gggatcgatc tggcccttgg ccagcgcgaa cttggccgac gtcggtgcgg
      901 cgtggtggtt gttgtgcaac gcctcgccgc cggtgaccag cgcgagcgac tgcaagttcg
      961 tcgcggaatt gtcgtagggc tgcttcccgt acgtgtgcgt cactgcgttg atggcgcccg
     1021 acatcatcac gaacgagacg gcgtgcagtc ccgcggccag caaaccgacg acgatgccga
     1081 agccgagccc ccatgtcacc gtgacgagca gcccaatacc gactcccaga ccgaagaggg
     1141 cgtggtcgaa gaacagacgg tcccacttgt cgcgcttgat gtcacgcgcg tacttgtgca
     1201 cctgcaactc gtcggacgcg accttcttgt agaggccgac gttgccgagt tgtacgcgcc
     1261 agaagccgag taccttcggg ctgtgcggat cctcgggggt gtcggttgcc gcgtgatgct
     1321 tgcggtgaac agccacccat tcacgcggct tcatgccggt ggtgatccag atgaagaagc
     1381 ggaacggcat cgccgcggcc ggcttcacgg tgagcgcccg gtgcgagagg ccccggtgca
     1441 agtagacggt ggtggccgca gtcgcgatct gggtgaccag gagcgcgaag gcgacgacgg
     1501 caatccattg agcagttcgc attggcctca caccctacta ccgactagtc ggtaggctgg
     1561 catcacctca tggcgatcgc agcgaattcc gaccccacca ccaaggacct gatcctggcg
     1621 gccgccaccc gccgcttcgc cgaccacggc tacgccggga ccagcctcaa cgacattgcc
     1681 gacgaggtcg gtatccggcg gccgagcctg ctgcaccatt acccctccaa ggaagtgctc
     1741 taccgcgcgg tgctctacag ctccttcgag gactggagcg gcctcgtcga cgaagcgatc
     1801 gtcggcaccc gcgaaggttg gccgcaggtt gagcggatgt tgcgcgccgc gttcacgttc
     1861 ttcgaagacc accccgactt cgtgcgcctc gtgcgatggg aagcgctcga aggcgggccg
     1921 cgcatcagcc acgagctttc gttgctgttg aagccgttgt tcgaccgcgg tgccgcgttc
     1981 ctcgaacgag agatggatgc cggccgcttg aagcgttatg acgcacgcca gttgttgctc
     2041 accggatacg gcgcggtgct ctcgtacctg tccgacggcc agttgatgag cggcctgctc
     2101 gacactgatc cgctctcgcc cgagatgttg gccgtccggc gggagcatgt gatcgcggtg
     2161 ctccggaccg cagtcgcgcc taccagttga cggtcgcgcg ccagcgcgtc aggacctcgg
     2221 cgtcgccgaa cacttcgagt tcgctcggct gcacgcggcc gtagaggaag agcaacaagt
     2281 cgttcgccgc gccgcgcgct gcgacatcgc ctttggc
//
LOCUS       DASUGA010000210         2060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1977252, whole genome shotgun sequence.
ACCESSION   DASUGA010000210 DASUGA010000000
VERSION     DASUGA010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1977252"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VFX21_04160"
     CDS             complement(<1..881)
                     /locus_tag="VFX21_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5095177.1"
                     /translation="MPNHPTRDDIDLLDGGFYIGTVRERYAWMRANAPVYFDAHNGLW
                     GFASYDAVLAAEKDAKTFSNAGGSRPVNPPLPMMIDMDDPDHWKRRKLVNKGFTPRRV
                     RDSEDWIRQVCDEIIDAVCERGECDFVNDVAAPLPMILIGDLLGVAPENRAELLQWSD
                     DMLTSLDTKATEEQITKSMEAATGYAEFCTHAVAQRQTNPTDDLMSILVHAEVDGDRL
                     SPEDILYESLLILIGGDETTRHVISGGMEQLILHPEQRKVLVDDPAKIEAGVEEMLRW
                     VTPIKSMCRTVTRDTTF"
     gene            complement(886..1647)
                     /locus_tag="VFX21_04165"
     CDS             complement(886..1647)
                     /locus_tag="VFX21_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005178341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5095178.1"
                     /translation="MTLENTTALVTGGASGMGEASARRLAEAGATVIVLDRDANKGEK
                     VAAELGGRFVQADVTDDGDVSAAVAAATALAPLRTCIHCAGIGWAERTINRTGAPHSL
                     DTFKKVIEINLIGTFNVLRLAASGMSANEPTEDGERGVIVNTASIAGYDGQIGQAAYS
                     ASKGGVIALTLTAARDLSAAGIRVCTIAPGLVDTPLLGTLPDDARNALAQSVLFPKRL
                     GVPDDFASLAMEIVRNRYLNGEVIRMDAGIRMPPK"
     gene            1686..1949
                     /locus_tag="VFX21_04170"
     CDS             1686..1949
                     /locus_tag="VFX21_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX5095179.1"
                     /translation="MRWPPDAFLKSVFPTGETPGPTTCFHVLSVIQVYADGECDVTTA
                     VAIAEHLDHCPICRDELASFRWLKAAVRRCGGASRAPESRAWP"
BASE COUNT          303 a          670 c          714 g          373 t
ORIGIN      
        1 aagaaggtcg tgtcgcgcgt gacggtgcgg cacatgctct tgatcggggt gacccagcgc
       61 agcatctctt cgacccccgc ttcgatcttg gcggggtcgt cgaccagcac cttgcgttgt
      121 tcgggatgca ggatgagttg ctccatgccg ccgctgatga cgtggcgggt cgtctcgtcg
      181 ccgccgatga ggatgagcag cgactcgtaa aggatgtctt ccggtgaaag gcggtcgccg
      241 tcgacctctg cgtgcacgag gatgctcatg agatcgtcgg tggggttcgt ctggcgttgt
      301 gcgaccgcat gcgtgcagaa ctcggcgtag ccggtcgccg cctccatcga cttcgtgatc
      361 tgctcctcgg tcgccttggt gtcgagcgac gtgagcatgt cgtcggacca ctgcaacaac
      421 tcggcccggt tctcgggcgc gacgccgagc aggtcgccga tgaggatcat cggcaggggt
      481 gccgcgacgt cgttcacgaa atcgcattcg ccgcgttcgc agaccgcgtc gatgatctcg
      541 tcgcagacct ggcggatcca atcctcgctg tcgcgcacgc gtcgcggggt gaagcctttg
      601 ttgacgagct tgcggcgctt ccaatggtcg ggatcgtcca tgtcgatcat catcggcagc
      661 gggggattga ccgggcggct accgcccgcg ttcgaaaacg tcttggcgtc cttttcggcc
      721 gcgagcaccg cgtcgtagga cgcgaagccc cacagcccgt tgtgggcgtc gaagtacacc
      781 ggcgcgttgg cgcgcatcca cgcataccgc tcgcgcaccg ttccgatgta gaacccgccg
      841 tcgaggagat cgatgtcgtc ccgggtgggg tggttcggca tgcccctact ttggcggcat
      901 ccggataccg gcgtccatcc ggatcacctc gccgttcagg tagcggttcc gcacgatttc
      961 catcgcgagc gacgcgaagt cgtcgggaac gccgaggcgc ttcgggaaca acaccgattg
     1021 cgccagcgcg ttgcgggcgt cgtcgggcag cgtgccgagc agcggcgtgt cgacgagccc
     1081 cggcgcgatc gtgcacacgc gaatgccggc cgccgagagg tcgcgtgcgg cggtgagtgt
     1141 cagcgcgatg acgccgcctt tggatgcgga gtacgcggct tggccgatct ggccgtcgta
     1201 acccgcgatc gacgcggtgt tgacgatgac gccccgctcg ccgtcttccg tcggttcgtt
     1261 ggccgacatc cccgacgccg caaggcgcaa cacgttgaac gtgccgatca agttgatctc
     1321 gatgaccttc ttgaacgtgt cgagcgagtg cggtgcgccg gtgcggttga tcgtgcgttc
     1381 ggcccagccg atccccgcgc agtgaatgca cgtccgcagt ggtgcgagcg cggtcgcggc
     1441 cgccaccgct gcgctgacgt cgccgtcgtc ggtgacgtca gcctggacga atcgcccgcc
     1501 cagttcggcc gcgacctttt cgcccttgtt ggcgtcgcgg tcgaggacga tcacggtcgc
     1561 gccggcctcg gcgaggcgcc gggcggacgc ttcgcccatg ccggaggcgc cgccggtgac
     1621 gagggcagtg gtgttctcga gggtcatggg ccgggactgt acgcactggg gcggagggag
     1681 cgggaatgcg atggccgccc gacgcgttcc tcaagagcgt gttcccgacc ggcgaaaccc
     1741 ccgggccgac gacgtgcttt cacgtccttt cggtcatcca ggtctacgcc gacggcgagt
     1801 gcgacgtcac gaccgcggtg gcgatcgccg agcatctcga ccactgcccg atctgccggg
     1861 acgaactcgc gtcctttcgc tggctgaagg cggcggtgcg gcgatgcggg ggtgcgagcc
     1921 gcgcgccaga atctcgggcg tggccctgat ctcatacgcc cagcagctct cgaatctcgc
     1981 cgccgccgac ggtgcgcatc tcgcggtcac cgacgaacac gaaagcgtga cgcgcgccga
     2041 gctcgaatcg atcgcgaacc
//
LOCUS       DASUGA010000211         3531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_170439, whole genome shotgun sequence.
ACCESSION   DASUGA010000211 DASUGA010000000
VERSION     DASUGA010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170439"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..677
                     /locus_tag="VFX21_04175"
     CDS             231..677
                     /locus_tag="VFX21_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX5095180.1"
                     /translation="MPTTTPADDEAALRAHGLSVTAQRLAVLRAVAGVSHRTADGIYT
                     VVRAELGSVSKQAVYDALATLTEKGLVRRIQPAGSPARYEDRVGDNHHHLICRVCGGM
                     VDVDCAVGDAPCLTADDAAGFEIDEAEVIYWGRCPDCMAAVRKPRP"
     gene            711..2903
                     /gene="katG"
                     /locus_tag="VFX21_04180"
     CDS             711..2903
                     /gene="katG"
                     /locus_tag="VFX21_04180"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007262102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEX5095181.1"
                     /translation="MSNGSESENPVIDSPTPHATRPHTNKDWWPNQLDLSVLHQHSPH
                     ANPMEEDFEYAKEFATVDLAELKRDIEQVMTTSQEWWPADYGHYGPLFIRMAWHAAGT
                     YRVADGRGGGGTGAQRFAPLNSWPDNANLDKARRLLWPVKQKYGRKLSWGDLIIFAGN
                     CALESMGFETFGFGGGRTDTWEPDEIFWGPEDTWLGDERYSGDRELATPFGAVQMGLI
                     YVNPEGPNGNPDPLAAAPDIRETFARMAMNDEETVALIVGGHTFGKCHGAHSADMIGP
                     EPEGCPIEAQGFGWHNPSGTGKGADAVTSGLEGAWTNNPTQWDNGFLDNLYKYEWELT
                     ESPAGAKQYTPTDKSSANDVPDAHDASKRHAPMMLTTDLAMRLDPIYAPIAKRFHEHP
                     EELADAFARAWYKLTHRDMGPISRYVGALVPSEQLIWQDPVPAVDHPLVGATEIAALK
                     EKLLASGLSTAQLVGTAWASAASFRGTDKRGGANGARIRLAPQRDWAVNEPAELGVVL
                     KTLEQIQQDFNATQSGGVKISLADLIVLGGCAAVERAAKDAGVNVTVPFVPGRTDATQ
                     EQTDIESFAVLEPRADGFRNYVSPGEKLSPETLLLERANLLTLTAPEMTVLVGGMRAL
                     NANCAQSTLGVFTERPGVLTNDFFVNLLDIGTEWKVSTTEHEYEGRDRATGELKWTAT
                     AVDLVFGSHSQLRGLAEVYASDDAQEKFVRDFVAAWNKVMDLDRFDLK"
     gene            complement(2904..>3531)
                     /locus_tag="VFX21_04185"
     CDS             complement(2904..>3531)
                     /locus_tag="VFX21_04185"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5095182.1"
                     /translation="TELPRVLPAPPNEPDALPIAGEMQALTRAIAFDGDWTAERKEQI
                     ASFFDAMAEGWRDRDAPVRHDPVLDALARGGPFPDGWCVEVGSGTGNVTADFRRAFAD
                     VVALDLSFEMLRLAPARSRQIQSDASMLPLRSASAAVLALVNMFVFPGEIARVLAGDG
                     VLLHVSTGGDHTPIYLSPADVMRVLPGEWEGVTAKAGWGTWLTARRRA"
BASE COUNT          626 a         1214 c         1199 g          492 t
ORIGIN      
        1 atccgttccg agcgggaccg gttggttcga gaacgcgacg aagcagtggc gcggcgcgac
       61 gaggtggtac ggcagttgcg caagctgctg cacgacgaag cggaggcttc ggccgagcat
      121 cacgctcgac cggccgccgc ggagtgacgg cgtccgcgcg acgccgcgac cgcattctgg
      181 gttgaatctg aatctggact cgatccagat tattcgatag ccttcgggcc gtgcccacca
      241 cgacccccgc cgacgacgag gccgcgctgc gggcgcacgg cctgtcggtg acggcgcagc
      301 gcctcgccgt cctgcgcgcg gtcgccggcg tctcccaccg cactgccgac ggcatctaca
      361 ccgtcgtgcg cgccgagctc ggctcggtgt cgaagcaagc ggtgtacgac gcgctcgcga
      421 cgctcactga gaagggactc gtgcggcgca tccagcccgc cggttcaccc gcgcggtacg
      481 aggaccgcgt cggcgacaac caccaccatc tcatctgccg cgtctgcggt ggcatggtcg
      541 acgtcgactg tgcggtcggc gacgcgccgt gcctgaccgc ggacgacgcg gcgggtttcg
      601 agatcgacga agccgaggtc atctactggg gtcgctgccc cgactgcatg gccgccgtac
      661 gcaagccccg cccttgacga ccttcgaacg aatgcacgag gaggactgac gtgtccaacg
      721 gcagcgagag cgaaaacccc gtcatcgatt cccccactcc gcacgcaacc cgaccgcaca
      781 ccaacaagga ttggtggccg aaccagctcg acctgtcggt actccatcag cactcgccgc
      841 acgcgaaccc gatggaggaa gacttcgagt acgcgaagga gttcgcgacc gtcgatctcg
      901 cggagctcaa gcgcgacatc gagcaggtga tgacgacgtc gcaggagtgg tggccggccg
      961 actacgggca ctacggaccg ctcttcatcc gcatggcgtg gcacgcggcg ggcacgtacc
     1021 gcgtcgcgga cggacgcggt ggtggcggca ccggtgcgca acggttcgcg ccgctcaaca
     1081 gctggcccga caacgccaac ctcgacaagg cccgccggtt gctgtggccg gtcaagcaga
     1141 agtacggccg caagctgtcg tggggcgacc tgatcatctt cgccggcaac tgcgcgctcg
     1201 agtcgatggg cttcgagacg ttcggcttcg gcggtggtcg caccgatacg tgggagcccg
     1261 acgagatctt ctggggcccc gaagacacgt ggctcggtga cgagcgctac agcggggacc
     1321 gcgagctcgc cacgccgttc ggtgcggtgc agatgggctt gatctacgtc aaccccgaag
     1381 gccccaacgg caaccctgac ccgctcgccg cggcgcccga tatccgtgag acgttcgcgc
     1441 gcatggcgat gaacgacgag gaaactgtcg cgctcatcgt cggtggtcac accttcggca
     1501 agtgccacgg cgcgcacagc gcggacatga tcgggcccga acccgagggt tgtccgatcg
     1561 aggcgcaagg tttcggctgg cacaacccgt cgggcaccgg caagggcgcg gacgcggtga
     1621 ccagcggtct cgaaggtgca tggaccaaca accccacgca gtgggacaac gggttcctcg
     1681 acaacctgta caagtacgag tgggagctca ccgagagccc ggccggcgcg aagcaataca
     1741 cgccgaccga caagtcgtcg gccaatgacg taccggacgc gcacgacgca tcgaagcgcc
     1801 acgcgccgat gatgctcacg acggatctgg cgatgcggct cgacccgatc tacgcaccga
     1861 tcgcgaagcg cttccacgag caccccgagg agctcgcgga cgcgttcgcc cgggcgtggt
     1921 acaagttgac gcaccgcgac atgggcccga tctcgcggta cgtcggtgcg ctggtgccca
     1981 gtgagcagct gatctggcag gaccccgtgc ccgcggtcga ccacccgctc gtcggcgcga
     2041 ccgagatcgc ggcgctgaag gaaaagctgc tcgcgtcggg cctctcgacc gcgcagctcg
     2101 tcggcaccgc gtgggcgtcg gccgccagct tccgcggcac cgacaagcgc ggtggcgcga
     2161 acggcgcccg catccgcctc gcgccgcagc gcgattgggc cgtgaacgag ccggccgagc
     2221 tcggcgtcgt gttgaagacg ctcgagcaga tccagcagga cttcaacgcg acgcagtccg
     2281 gtggtgtgaa gatctcactc gccgacctga tcgtgctcgg tggctgcgcg gcggtcgagc
     2341 gggcggcgaa ggacgccggc gtgaacgtca cggtgccgtt cgtgccgggt cgcaccgacg
     2401 cgacgcagga gcagaccgac atcgagtcgt tcgcggtgct cgaaccgcgc gccgacggct
     2461 tccgcaacta cgtcagcccg ggagagaagc tctcgccgga gacgttgctg ctcgaacgag
     2521 cgaacctcct gaccctgacc gcgccggaga tgacagtgct ggtcggcggg atgcgggcac
     2581 tgaatgcgaa ctgcgcgcag tcgacgctgg gcgtcttcac cgagcggccc ggtgtgttga
     2641 cgaacgactt cttcgtcaat ctgctcgaca tcggcacgga gtggaaggtg tcgacgaccg
     2701 agcacgagta cgaaggccgc gaccgagcca caggcgagct gaagtggacc gcgaccgcag
     2761 tcgacctcgt cttcggttcg cactcgcaac tccgaggcct ggccgaggtc tacgcgagcg
     2821 acgacgccca ggagaagttc gtccgcgact tcgtcgccgc gtggaacaag gtgatggacc
     2881 tcgaccgctt cgacctgaag tgatcaggcg cggcggcgcg cggtgagcca cgtgccccaa
     2941 cccgccttgg cagtgacgcc ttcccattcg cccggcagca ctcgcatcac gtcggcgggg
     3001 gagagataga tcggcgtgtg gtcaccgccg gtgctcacat gcaacaacac gccgtcaccg
     3061 gccaggacgc gcgcgatctc accggggaac acgaacatgt tgaccagcgc gaggacggcc
     3121 gcgctcgccg accgcagcgg cagcatcgac gcgtcggatt ggatctgccg actacgcgcc
     3181 ggcgcgagcc gcaacatctc gaacgagagg tccagcgcca cgacgtccgc gaacgcgcgc
     3241 cggaaatcgg cggtcacgtt gccggtgccc gagccgacct cgacacacca accgtcgggg
     3301 aacgggccac cgcgcgccag cgcgtcgagc accggatcgt gccgcacggg cgcatcgcga
     3361 tcccgccaac cctcggccat cgcgtcgaag aacgacgcga tctgctcctt gcgttccgcc
     3421 gtccagtcgc cgtcgaatgc gatcgcgcgc gtcaacgcct gcatctcacc ggcgatgggc
     3481 agcgcatcag gttcgttcgg cggcgcgggc agcacgcgcg ggagttcggt g
//
LOCUS       DASUGA010000212         3959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1977163, whole genome shotgun sequence.
ACCESSION   DASUGA010000212 DASUGA010000000
VERSION     DASUGA010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1977163"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VFX21_04190"
     CDS             <1..694
                     /locus_tag="VFX21_04190"
                     /EC_number="1.1.1.333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007458997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="decaprenylphospho-beta-D-erythro-pentofuranosid-
                     2-ulose 2-reductase"
                     /protein_id="HEX5095183.1"
                     /translation="TCEKLARRRHARVVLAGRKPESLESAATAIKRAGASEVHTVAFD
                     ATDFATHEQFVAQTFDTYGDFDVVLVAFGVLGEQERAETDAAVALEIVQTNFTGVVSV
                     TVPLANRLQAQGHGTLILLSSVAGERARRSNFVYGSSKAGVDAFYQGLGDSLTDDGVQ
                     VMVVRPGFVHTKMTAGLAAAPLATTPEKVADAIVRGIERGSETVWVPAPMRFVMSGLR
                     HLPRPVFRKLEV"
     gene            691..1323
                     /locus_tag="VFX21_04195"
     CDS             691..1323
                     /locus_tag="VFX21_04195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEX5095184.1"
                     /translation="MSGPTRTLAAFDVDGTLTARDTLAPFLARIVGRTALVRAAAANA
                     PRLAPIALGRGDRDATKERMLVRLLRGRPYEPIATAGAAYGREIAADRVAPAMHERIA
                     WHLEQDHEVVLVSASLGTYLDEVARVLGASTALCTRLEVDDDGHCTGRMIGGNCRGAA
                     KADRLLAHIGRGDATIYAYGDSRGDDELLALADHAFRVRHTRGGVRCTEE"
     gene            complement(1334..2965)
                     /locus_tag="VFX21_04200"
     CDS             complement(1334..2965)
                     /locus_tag="VFX21_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HEX5095185.1"
                     /translation="MNETDGRAPSGHDDCPACRSHAGQAQRAKSESQISRRRALKMAA
                     GAGAAAAVVPHLSLFGGETTPRLTDLRNARPTLAQRPDWPAPAIITRAQWGANEAIRK
                     HDQTYEGVIEKIVVHHTGTPNPVNDGAAHLRGIYESAISSGVYSDVQYHWLIDPQGRI
                     YEGRWATDYPPGAPHTGELNGQNVRGGHAENYNSRTIGVAIMGDYSNFAPPAAAIDSL
                     IALLTWKCARWGIDPGGASPYVNGLGATVSIANITSHRETKNSFGDGTTCPGDPLINM
                     LPSIRASVASRLADGSTGYWVASKEGRLLSFGSLPDMGDTRRLGIPSQIMGLSAHPSG
                     LGYWLVGRDGGVFTFGAAQFFGSMGGVRLNQPMIGMSSTTTGNGYWLVALDGGVFSFG
                     DARFFGSMGAVRLNAPILGLCPTPSGAGYWLYARDGGIFSFGDARFFGSTGGLRLNKP
                     IVAMAARPQGDGYWLVAEDGGVFSFGAAQFFGSGASYSLRGPVVGMVPTTTGNGYVLL
                     VRDGGVLAFGDAPFLGSAAGRVLEAVGIAGRLKPV"
     gene            3087..>3959
                     /locus_tag="VFX21_04205"
     CDS             3087..>3959
                     /locus_tag="VFX21_04205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5095186.1"
                     /translation="MPATARPTVAFDVTPLLGVRTGIGNAVAEMYAALQGLDAAPDLV
                     PYTLSLRARQMREDVPSDTRFVPLPARGLLKAWARANTPRIDRWITPARVVHATNYLA
                     PATRLPTVVSVYDCSFVRYPELCTPEVLAFVPILRRAIERGATVHTTSEFVATEIEEA
                     FGARRRDGKLVVVPLGVPPLGTEAAMPADLRDELDGAPYVLAVGMLEPRKNFPHLIGA
                     FGALAASHPELRLVIVGPDGPARPSVERAVERLPAAVRPRVLLPGAVDNLARRALLDA
                     AAVLAYPSIYEGFGF"
BASE COUNT          594 a         1386 c         1407 g          572 t
ORIGIN      
        1 gacgtgcgag aagctcgcac ggcgccggca cgcgcgcgtc gtcctcgcgg gccgcaagcc
       61 ggagtcgctc gagagcgccg cgaccgcgat caagcgcgcc ggcgcatccg aggtgcacac
      121 ggtcgcgttc gacgccaccg acttcgccac ccacgagcag ttcgtcgcgc agacattcga
      181 cacctacggc gacttcgacg tcgtgctcgt cgcgttcggt gtgctcggcg aacaggagcg
      241 agccgagacc gatgccgccg tcgcgctcga gatcgtgcag acgaacttca ccggcgtcgt
      301 gtcggtgacc gtgccgctcg ccaaccggct gcaggcgcag ggccacggca cgctgatcct
      361 gctgtcgtcg gtcgcgggcg aacgggcccg gcgcagcaac ttcgtctacg gctcgtcgaa
      421 ggcgggcgtc gacgcgttct accaagggct cggcgactcg ctcaccgacg acggcgtgca
      481 agtcatggtc gtgcgtcccg gcttcgtgca cacgaagatg acggcgggcc tcgcggctgc
      541 accgctcgcc acgacgccgg agaaggttgc cgacgcgatc gtgcggggca tcgaacgcgg
      601 ctcggagacg gtgtgggtgc cggcgccgat gcgcttcgtc atgtcggggc tgcggcatct
      661 cccgcgaccg gtgttccgca agctcgaggt gtgagcggtc cgacgcgcac actggctgcg
      721 ttcgacgtcg acgggacgtt gaccgcgcgc gacacgctcg caccgttcct cgcgcgcatc
      781 gtcggccgga cggcgctggt gcgggccgcg gccgcgaacg cgccgcggct tgcgcccatc
      841 gcgctcggcc gcggtgaccg tgacgcgacg aaggagcgga tgctcgtgcg cctcttgcgc
      901 ggccggccgt acgagccgat cgcgacggcc ggcgccgcat acgggcggga gatcgccgcc
      961 gaccgagtcg cgcccgcgat gcacgaacgg atcgcgtggc acctcgaaca ggaccacgaa
     1021 gtcgtgctcg tctccgcgtc gctcgggacg tacctcgacg aggtcgcgcg cgtgctcggc
     1081 gcgtccacgg cgctgtgcac acgactcgaa gtcgacgacg acggccactg cacgggccgc
     1141 atgatcggcg gcaactgccg cggtgcggcc aaggccgatc ggctgctggc gcacatcggc
     1201 cgcggcgatg cgacgatcta cgcgtacggc gacagcaggg gcgacgacga actgctcgcg
     1261 ctcgcggacc acgcgttccg cgtgcggcac acacgcggcg gcgtgcgctg taccgaggaa
     1321 tgagggtcgc ggctcagacg ggtttgaggc ggcccgcgat gccgaccgct tcgagcacgc
     1381 ggcccgcggc gctgccgagg aacggcgcgt caccgaacgc gagcacgcca ccgtcgcgta
     1441 cgaggagcac gtagccgttg ccggtcgtcg tcggcaccat gcccacgacc ggaccgcgca
     1501 acgagtagct cgcgcccgaa ccgaagaact gcgcggcgcc gaacgagaac acgccgccgt
     1561 cttcggcgac gagccagtac ccgtcgccct gcgggcgcgc ggccatcgcc acgatcggct
     1621 tgttgaggcg cagaccgccg gtcgaaccga agaagcgcgc gtcgccgaag ctgaagatgc
     1681 cgccgtcacg cgcgtacaac cagtagcccg cgcccgacgg cgtcgggcac aacccgagga
     1741 tcggcgcgtt gagcctcacc gcgcccatgg agccgaagaa gcgggcgtcg ccgaaggaga
     1801 agacgccacc gtccagcgcg acgagccagt agccgttgcc ggtcgtcgtc gacgacatgc
     1861 cgatcatcgg ctggttcagg cggacaccgc ccatagagcc gaagaactgc gcggcgccga
     1921 aggtgaacac gccgccgtcg cggcccacca gccagtagcc gagccccgac ggatgcgcgc
     1981 tgagccccat gatctgcgat gggatcccga ggcggcgggt gtcgcccatg tcggggaggc
     2041 tgccgaagct gagcagccgg ccttctttcg acgcgaccca gtagccggtg gatccgtcgg
     2101 cgagccggga cgcgaccgag gcgcggatcg acggcagcat gttgatgagc gggtcgcccg
     2161 ggcacgtggt gccgtcgccg aaggagttct tcgtctcgcg atgcgaggtg atgttcgcaa
     2221 tggaaacggt cgcgccgagc ccgttgacgt agggtgatgc gccacccgga tcgatgcccc
     2281 accgcgcgca cttccacgtc agcaacgcga tcagagaatc gatcgcggcg gccggcggag
     2341 caaagttcga gtagtcgccc atgatggcga cgccgatcgt gcgcgagttg tagttctcgg
     2401 cgtgaccacc gcggacgttc tggccgttca gttcgccagt gtgcggcgcg cccggcggat
     2461 agtcggtggc ccaacgtcct tcgtagatgc ggccttgcgg atcgatgagc cagtggtatt
     2521 gcacatcgct gtagacaccg ctgctgatcg cgctctcgta gatgccacgc aagtgcgctg
     2581 cgccgtcatt gacaggattc ggcgttcccg tgtgatgcac gacgatcttc tcgatgacac
     2641 cttcatacgt ctggtcgtgc ttgcgaatcg cctcgttcgc accccactgc gcgcgcgtga
     2701 tgatggccgg cgcaggccag tcggggcgct gggcgagggt cggccgggcg ttgcgcaggt
     2761 cggtgaggcg cggggtcgtc tcgcccccga acaacgacag gtgtggcacg acggccgcgg
     2821 ccgcaccggc gccggccgcc atcttgagcg cgcgccgacg cgagatctgc gactcgctct
     2881 tcgcccgttg ggcctgtccc gcgtgcgacc ggcacgcggg acagtcgtcg tgcccgctcg
     2941 gcgcacgacc gtcggtttca ttcacgggtt ccccccagca cgtccgcgaa ttccatcggc
     3001 agggcacagg gaagtacgaa gcatacggga tgcgcggacg acgacggcgg ctcccggcgt
     3061 acggcgcagt gtggtacgaa cggcacgtgc ccgcgaccgc ccgtccgacc gtcgcgttcg
     3121 acgtcacgcc gctcctcggc gtgcggaccg gtatcgggaa cgccgtcgcg gagatgtatg
     3181 cggccttaca gggactggac gccgcgcccg acttggtgcc gtacacgttg agcttgcgcg
     3241 cgcgtcagat gcgggaggac gttccgtccg acacgcggtt cgtgccactc cccgcgcgcg
     3301 ggctgctcaa agcgtgggcg cgcgcgaaca cgccgcgcat cgaccgttgg atcacaccgg
     3361 cgcgcgtcgt gcacgccacg aactatctcg cgcccgcgac gcggctgccg acggtggtca
     3421 gcgtgtacga ctgttcgttc gtgcgttacc ccgagttgtg cacacccgag gtgctcgcgt
     3481 tcgtcccgat cctgcggcgg gcaatcgaac gaggcgcgac ggtgcacacg acctcggagt
     3541 tcgtggcgac ggagatcgag gaggccttcg gcgcccgccg ccgcgacggc aagctggtcg
     3601 tcgtcccgct cggggtcccg ccgctcggca ccgaggcggc catgccggcc gatctgcgcg
     3661 acgaactcga cggcgcgccg tacgtcctcg ccgtcgggat gctcgagccg cgcaagaact
     3721 tcccgcacct gatcggcgcg ttcggcgcgc tggccgcgtc gcatcccgag ttgcggctcg
     3781 tcatcgtcgg acccgacggg cccgcgcgcc cgagcgtcga gcgcgcggtc gaacgactgc
     3841 ccgccgcggt gcgaccgcgc gtgttgctac cgggcgcggt cgacaacttg gcgcgccgcg
     3901 cgctgctcga cgctgcggcc gtcctcgcgt acccgtcgat ctacgaaggg ttcggcttc
//
LOCUS       DASUGA010000213         2250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_848419, whole genome shotgun sequence.
ACCESSION   DASUGA010000213 DASUGA010000000
VERSION     DASUGA010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848419"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VFX21_04210"
     CDS             complement(<1..448)
                     /locus_tag="VFX21_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095187.1"
                     /translation="MATAALVALTVGVGAYAFEMWRSRGPDEASVSEAVTRFRSSSTT
                     VASEAMRVRPAAGVYTYAGSGEEELSFAGTHQTEGPSIPGTVTYGDNGCWTFQVEYNT
                     FHRQTWNWCTRGGRVVELGGTTQQKFDFVAFKVDEQSTITCDPPFTA"
     gene            complement(541..1179)
                     /locus_tag="VFX21_04215"
     CDS             complement(541..1179)
                     /locus_tag="VFX21_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5095188.1"
                     /translation="MTATTFLLLFFFVWFVVGLATAAVMARRGHDLWTWVMLGLLMGP
                     LVLALVIPAVHRESHLAPETRSLHHGGGGPGAVSVLIGTDGSPDAEIAAATVSRLFGP
                     QLGRVTVATVVDFDAADLAGAKATVTTRASELLEDTAALLEPVDADRVVLTGRPSDAL
                     LAYAIEHDVDLIVVGQRGAGLTPAVVGSTADQLVRQRRVPVLLTGVPECVHS"
     gene            1432..1812
                     /locus_tag="VFX21_04220"
     CDS             1432..1812
                     /locus_tag="VFX21_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5095189.1"
                     /translation="MRIATLIGGEVVFVAPDATLADVIDVLAANEIGVVVVGGPDEPL
                     GVVSERDIVHALADRRPIESTRAADVATNKLVWCDAHSLVGDVAAEMMESYVRHVLVE
                     KNGRLVGIVSARDLLGFYAEMSSS"
     gene            1848..>2250
                     /locus_tag="VFX21_04225"
     CDS             1848..>2250
                     /locus_tag="VFX21_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5095190.1"
                     /translation="MDLTFWGAAETVTGSRFVVDTVAARVLVDCGLFQGLKRLREQNW
                     KPFAIDQASIDAVVLTHAHLDHSGYLPALIRDGYRGPVWCTPGTAALAEILLLDAARI
                     QEEDARFANRRRSSKHHPALPLFTTADAERAL"
BASE COUNT          365 a          762 c          771 g          352 t
ORIGIN      
        1 tggccgtgaa cggcggatca cacgtgatcg tgctctgctc gtcgaccttg aacgcgacga
       61 agtcgaactt ctgctgtgtc gtcccgccga gctcgacgac ccgtccaccg cgtgtgcacc
      121 agttccacgt ctgacggtga aaggtgttgt attcgacctg gaaggtccag caaccgttgt
      181 cgccgtaggt gaccgtgccg ggaatcgacg gtccctccgt ttggtgcgta cccgcgaacg
      241 agagctcctc ctccccggag cccgcgtacg tgtacacgcc ggccgcgggc cggacgcgca
      301 tcgcctccga cgcgacggtc gtcgaggacg accggaaccg agtgaccgcc tccgacaccg
      361 atgcttcgtc ggggccgcgc gatcgccaca tctcgaacgc gtaagcgccg acgccgacgg
      421 tcagcgcgac cagggccgcg gtggccacga tccatgcccg ccgccgcggc cggcggcgta
      481 ccggagcggg cgtcgccgcg gtgtctgacg cgatctcttc cagggtctct ctcgcggtgt
      541 ctacgagtgc acgcattccg gtacccccgt gagcaggacg ggcacgcgcc gctgccggac
      601 gagctggtcg gcggtgctcc cgacgactgc gggcgtgagc cccgcgccgc gctgaccgac
      661 cacaatcagg tcgacgtcgt gttcgattgc gtacgcgagc aatgcatccg atggccggcc
      721 cgtgagcacg acacgatcgg cgtcgaccgg ctcgagcagg gccgcagtgt cctcgagcag
      781 ctcgctcgcc cgagtggtca ccgttgcttt ggcgccggcc aggtcggcgg cgtcgaagtc
      841 gacgacggtc gcgactgtca cccgcccgag ctgcgggccg aacagccgcg agacggtggc
      901 ggccgcgatc tcggcatcgg gcgaaccatc ggtgccgatc aggacggaca cggctccggg
      961 tccgccgccg ccgtggtgca acgatcgcgt ctcgggagcc aggtgcgact cacggtgcac
     1021 ggcggggatc acgagcgcga gcacgagcgg ccccatgagc agaccgagca tgacccaggt
     1081 ccacagatcg tggccgcgtc gggccatcac cgcggccgtt gccaacccga cgacgaacca
     1141 gacgaagaag aagagcaaga gaaacgtcgt tgccgtcatc gcgatgctcc tgtgatcggc
     1201 gcacggtggt cgcctcgaac tacagactca tcgtcggcgc gcaacccgcg cccgggctag
     1261 ggacgttcga ccctgccgat ccgggccctt gtgggcatca cgtcggacgg agtgccattc
     1321 gaccctggtc gcagccaccc gatccgacga tcgttcgcat cgaagggagc agtcggcagc
     1381 gggcaccgga cacgaccgcg acgggccccg tctcgcaagg agggtcgaac catgcgcatt
     1441 gccacactca ttggcggcga ggtcgtcttc gtcgcacccg acgcaacgtt ggccgacgtg
     1501 atcgacgtgc tcgccgcgaa cgaaatcggt gtggtggtcg tcggcgggcc cgacgaaccc
     1561 ctcggcgtcg tttccgagcg agacatcgtg cacgcactgg cggatcgacg gccgatcgaa
     1621 tcgacgcgcg ccgctgacgt cgcgacgaac aaactcgtct ggtgcgacgc gcactcactg
     1681 gtcggcgatg tcgctgccga gatgatggag tcgtacgtgc gtcatgttct cgtcgagaag
     1741 aacggccgac tcgtcggtat cgtttccgcc cgcgatctgt tgggcttcta cgcggagatg
     1801 tcgtcgtcct gagctcggag atcccgaagc gcggcgtgag gaacgacatg gatctgacct
     1861 tctggggtgc cgcggagaca gtgaccggtt cgcggttcgt cgtcgacacg gtggcggcgc
     1921 gcgtgctcgt ggactgcggc ctgttccagg gtctgaaacg gctgcgcgag cagaactgga
     1981 agcccttcgc catcgaccag gcatcgatcg acgcggtggt gctgacgcac gcgcacctcg
     2041 accacagcgg ctatctgccg gcgctgatcc gcgacggtta ccgcggaccg gtgtggtgca
     2101 cgcccgggac cgcggcgctc gccgagatct tgttgctcga cgcggcgagg atccaggagg
     2161 aggacgcgcg gttcgcgaac agacgccggt cgtccaagca ccatcccgcg ttgccgttgt
     2221 tcacgaccgc ggatgccgag cgcgcgctcg
//
LOCUS       DASUGA010000214         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_448449, whole genome shotgun sequence.
ACCESSION   DASUGA010000214 DASUGA010000000
VERSION     DASUGA010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448449"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2004)
                     /locus_tag="VFX21_04230"
     CDS             complement(<1..>2004)
                     /locus_tag="VFX21_04230"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5095191.1"
                     /translation="EMALGDKDYVDDIRMPGLLHAAVVLSEHARADILAIDTAAAAAE
                     PGVVRVFTAADVPGELRVGLIHRDWPVFIPVGGRTSYVGDIVALVVAEDRATARAAAE
                     KVAVEYRPLRPITDAVAAVDDGEDAVWQLDGNVLSRSAFTRGDVDAALAGSAHVVHEV
                     FQTQRVEQAFLEPEATLAAPMDDGRLEVWSGGQGVWDDRRQIADVLGVDEQQIVVHMV
                     SNGGAFGGKEDCTNQCQTALAAWLLGRPVKCTFSREESLRVHAKRHPIRIEAWAGCDA
                     DGRLTALRARMIGDSGPYASVGMKVLERAAGHAGGPYAFPAMDVEALAVRTNNSVCGA
                     FRGFGANQAQFAVEGLLDRLAAAAGIDRLEFRAANLVDPGDVWGPGQVMDDGCAGARA
                     CLEEVRPDYDAAVAAGKAVGVAVGIKNSGLGNGLVEVSRAVVWLDPDGTVEVRHCWTD
                     MGQGVDTVALQVAVEELRVDPARVRVVVDTTRELGAGQTTGSRGTLMCAGAVADACRA
                     AVAAGCACGVESPGEFRVDWTQAIGATEHPTIHAAFGYAAQLVVMDRATGTIERVVAA
                     HDVGRAVNPLLCEGQVLGSVHMGLGYALSEEFPTGADGHPTAMTLKKLAILRPKDMPP
                     VDVRLVEVPQPRAPYGVKGVGEIGLVPTAGAVGSALHDLDGQWR"
BASE COUNT          289 a          780 c          668 g          267 t
ORIGIN      
        1 cgccactgcc cgtcgagatc gtgcagcgcg gatcccaccg cgccggccgt cgggacgagc
       61 ccgatctcgc cgacgccctt cacaccgtac ggcgcgcgcg gctgcgggac ttcgacgagc
      121 cgcacgtcga ccggcggcat gtccttgggc cgcaagatcg cgagcttctt cagcgtcatc
      181 gccgtggggt gcccgtcggc gccggtgggg aactcctcgg agagcgcata gccgagcccc
      241 atgtggaccg agccgagcac ctgcccttcg cacaacagcg gattcacggc gcggccgaca
      301 tcgtgcgcgg cgacgactcg ctcgatggtg cccgtggcgc ggtccatgac gacgagctgc
      361 gccgcgtacc cgaacgcggc atgaatcgtc ggatgctcgg tcgcgccgat ggcctgggtc
      421 cagtcgacgc ggaactcacc gggagactcg acgccgcacg cgcacccggc cgcgaccgcg
      481 gcgcgacacg cgtcggcgac ggcgcccgcg cacataagcg tgccgcggct gccggtcgtc
      541 tgccccgcgc cgagctcgcg cgtcgtgtcg acgacgaccc gcacccgcgc cggatcgacg
      601 cgcagctcct cgacggcgac ctgcagcgcg accgtgtcga cgccctggcc catgtcggtc
      661 cagcagtgcc gcacctcgac cgtgccgtcg gggtcgagcc acacgaccgc gcggctcacc
      721 tcgacgagcc cgttgccgag gccggagttc ttgatgccga ccgcgacgcc gacggccttg
      781 ccggccgcga ccgcggcgtc gtagtcggga cggacttcct ccaaacacgc gcgcgcaccc
      841 gcgcagccgt cgtccatcac ttgccccggt ccccaaacgt cgccgggatc gacgagattc
      901 gcggcgcgga actcgagccg gtcgatgccc gccgccgcgg cgaggcggtc gagcaacccc
      961 tcgacagcga actgcgcctg gttggcgccg aagccccgga atgcaccgca caccgagttg
     1021 ttcgtgcgca ccgcgagcgc ttcgacgtcc atcgcgggga acgcgtacgg gccaccggcg
     1081 tgacccgccg cgcgctccaa caccttcatg ccgaccgacg cgtacggtcc cgagtcgccg
     1141 atcatccgcg cccgcaatgc agtcagccgc ccgtcggcgt cgcatccggc ccacgcctcg
     1201 atccggatcg gatgccgctt ggcgtgcacg cgcagcgatt cctcgcggct gaacgtgcac
     1261 ttcaccggcc ggcccagcaa ccacgccgcc agcgcggtct ggcactggtt cgtgcagtct
     1321 tccttgccgc cgaacgcgcc accgttcgag accatatgca cgacgatctg ctgctcgtcg
     1381 acgccgagga cgtccgcaat ctggcgccgg tcgtcccaga cgccctgccc acccgaccac
     1441 acctcgagcc ggccgtcgtc catcggcgcc gcgagtgtcg cttccggttc gaggaaggct
     1501 tgttcgacgc gctgggtctg gaacacctcg tgcacgacgt gcgcgctgcc ggcgagcgcg
     1561 gcgtcgacgt cgccacgcgt gaacgcagac cgcgacagca cgttcccgtc gagctgccag
     1621 acggcatcct caccatcgtc gacggccgcg accgcgtcgg tgatcggccg cagcggccgg
     1681 tactcgaccg cgaccttctc ggccgcggcg cgcgccgtcg cgcggtcctc ggcgacgacc
     1741 agggccacga tgtcgccgac gtacgacgtg cgcccgccca ccggaatgaa cacgggccag
     1801 tcgcggtgga tgaggccgac gcgcaactcg cccggcacgt cggccgcggt gaagacgcgc
     1861 accacgccgg gctcggccgc ggccgcggcc gtgtcgatcg cgaggatgtc ggcgcgtgcg
     1921 tgctccgaca gcacgaccgc ggcatgcagc aatcccggca tgcggatgtc gtcgacgtag
     1981 tccttgtcgc cgagcgccat ctcg
//
LOCUS       DASUGA010000215         7736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2038330, whole genome shotgun sequence.
ACCESSION   DASUGA010000215 DASUGA010000000
VERSION     DASUGA010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7736
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2038330"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VFX21_04235"
     CDS             <1..489
                     /locus_tag="VFX21_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095192.1"
                     /translation="LAHADFNPVLAAVSALVAVIGVLISYAYYAGRLKVFENLSSRNK
                     VAHAGKTFLVNKYYLDALYEDVIVAGIKGPIANGAYWFNQKVIDNVLNYTGRGAQALG
                     RFTYDYIDQKGVDGIVNGIGTGTDEAGGAVRQIQTGRLQFYALLLVIAVGVFALALWI
                     AT"
     gene            505..2070
                     /locus_tag="VFX21_04240"
     CDS             505..2070
                     /locus_tag="VFX21_04240"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX5095193.1"
                     /translation="MEWKEWALSLAVFLPAVGAVIVMLIPRAQEEAIKWVTLIATVAT
                     LGVVIGILADFDYDYVTPEGGSGLQFELNNKWIEVINSRYHIGVDGISLPLLALSAFI
                     TVLCVVYSWNHFPEPHNPKAFLALMLILEVGMNGTFVAQDLILFFIFFEIVLLPMFFM
                     IGVWGGPNREYASIKFFLFTLFGSALMLLSFLALYFKVDTDIGHTFDMVALTDAAGSG
                     VVHSTQLVIFAGLFLGFAIKVPMFPFHTWLPDAHTEAPTVGSVLLAAILLKLGTYGFV
                     RIAFPMLPDAAESWAPWIGLLAVIGIIYGALGCLAQRDMKRLIAFSSVAHMGFVMLAI
                     ATMTTAGTNAAIFGMVAHGLITGMLFFIAGSVQHNFETRELSRLGGLLIQAPKMGWIL
                     GFCAMASLGLPGLAGFWGEFPALLSAFEPGPGLSDELFRTFMVIGAIGTVLAAGYLLW
                     MLQKVAFGTPKPEFADAHIHDVNPFEWAAWLPILLLILVLGIYPNLLFKVTDQTVSQG
                     PGQTTIVAEQANE"
     gene            2072..3559
                     /locus_tag="VFX21_04245"
     CDS             2072..3559
                     /locus_tag="VFX21_04245"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX5095194.1"
                     /translation="MNPTFDWHALAPEIVVSATLLVALLADMFSKRRDIVLVSRIASF
                     GVLGALIPIMTLAASDHDTRSMFGGAYVSDNYSLTLQAFFLVAAYVTILLSIDYIDEG
                     DYYKGEYFFLLLSSTLGMMVMASARDLITIFVALELISIPTFVLAAFRKHDRRSNEAG
                     VKYYLIGVLSSAIMLYGMSLIFGLYGTTVLSQINQQVSAEGAEPLLSVAIFLALVGFA
                     FKVSAVPFHFWAPDTYEGAPTPVTAFLSVSSKAGGFVALLSIIFFGFYGVDGAGSDLW
                     WGAVWVLAVLSMTLGNLAALRQTNIIRMLAYSSVAQGGFILVPFAAAGIAGADGNTDV
                     AATAFSAVVIYLLIYGAMNLGAFAVVIAAARRTRSAEVSSFAGLFTTSPVLSVLMTIF
                     MASLAGIPPLAGWFAKFVMFRSTLEAGTTWAVVLAVIAAVNSVIAFFYYARVIRVMWF
                     HEPAEGAGPVQVPGALAAALAITTAVTVVVGIYPQVFARVGELAF"
     gene            3561..4730
                     /locus_tag="VFX21_04250"
     CDS             3561..4730
                     /locus_tag="VFX21_04250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5095195.1"
                     /translation="MSAAEEIARRIRREGPIPFETFMEHALYEPDGGFFTSGGGAGRA
                     GRDFITSPEVGSLYGACVAESLTRGWREMGEPDPFVVVEAGAGNGRLAADVLRVQPDF
                     ASALRYVLVERSPRLRDEQRARLPLEPRDEALGPFVERDDRAEPLPRRGPIVASIPDL
                     PAITFEGIVFANELLDNLPFGIAAFDGTRWNEVRVTVDGDCFVEVLVPLVDDHALPDV
                     GAGARVPIPHALTAWFADLATVLRRGYVLLVDYIVTMDELLARGDDWLRTYRGHGSGN
                     DPLSEPGSSDITGDVVREQLLRAAATAGFTLVAEHTQADWLRGLGIDALVDEGRRAWE
                     EGAARGDLAAVAGRSRVNEAAALTDPAGLGAHRVVLLARSAQSPGFDRMTSFLSY"
     gene            4844..6799
                     /gene="acs"
                     /locus_tag="VFX21_04255"
     CDS             4844..6799
                     /gene="acs"
                     /locus_tag="VFX21_04255"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016825247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX5095196.1"
                     /translation="MTETLAALLQEGRTFPPSPEFRKDALVTEAKVYDESERDWQGFW
                     AKQALQLDWSKEWHTILEWDLPFAKWFVGGELNVAHNCLDRHVAAGHGDQIAYYWEGE
                     PGDTREVTYQQLLDESSRVANVLRSLGVAKGDRVAIYMGMVPEAAAAMLACARIGAPH
                     SVVFGGFTAASLRDRIIDAEAKVLITGDGAWRRGGVVPLKDIADEAIAETPSIEHVLV
                     LRRTENDVAMKDGRDIWWHDAVPAASTDCPVEPMDSEDLLYILYTSGTTGKPKGIMHT
                     TGGYLTQCAYTHKYVFDLHPDTDIFWCTADVGWVTGHSYIVYGPLANRATSVMYEGTP
                     DYPDKDRFWSIIAKYKVTKFYTAPTAIRTFMKWGDDYPARHDMSSLQLLGSVGEPINP
                     EAWVWYFKTIGDERCPVVDTWWQTETGAIMISPLPGATTLKPGSATFPLPGIKAEICD
                     DDGKAVGIPGGGYLVLTRPWPAMLRGIWGDPQRYRDTYWSRFSDRYFAGDGAKRDEDG
                     YFWLLGRVDDIMLVSGHNISTTEVEHALVDHESVAEAAVVGKVDETTGQAIAAFVILR
                     AGFEPSDALVEELRDHVAKLIGPIAKPKSVLFTEELPKTRSGKIMRRLLRNVAEDEAL
                     GDTTTLADPAIVDGIKTKYLATQPSEE"
     gene            6800..7291
                     /locus_tag="VFX21_04260"
     CDS             6800..7291
                     /locus_tag="VFX21_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095197.1"
                     /translation="MTRYLPEHWTWHNGPVEPGRAVVVDIDGVLSDATERQHYIEAPR
                     ANWRAFFDACGDDPVIEEVRTLLDLLDPDLQVVLLTARPHRLHDLTEAWLHRYEIRWN
                     LLIMRPWGDYELSRDFKQSTVWELRSAGFALALAFEDDRRNVEMFRAEGIPCLYVHSG
                     YYD"
BASE COUNT         1203 a         2587 c         2567 g         1379 t
ORIGIN      
        1 ctggcccacg ccgacttcaa ccccgtgctc gcggcagtgt cggcgctcgt cgcggtgatc
       61 ggggtgctga tctcgtacgc gtactacgcg ggccggctca aggtgttcga gaacctctcg
      121 tcgcgcaaca aggtcgcgca cgcgggcaag accttcctcg tcaacaagta ctacctcgat
      181 gcgttgtacg aggatgtcat cgtcgcgggc atcaagggcc cgatcgcgaa cggggcctac
      241 tggttcaacc agaaggtcat cgacaacgtc ctcaactaca ccggccgggg cgcacaggcg
      301 ctcggccgtt tcacgtacga ctacatcgac caaaagggtg tcgacggcat cgtcaacggc
      361 atcggtaccg gtacagatga agccggcggc gcggtgcgcc agatccagac cggccgactg
      421 cagttctacg cactgttgtt agtgatcgcc gtgggcgtgt tcgcgctcgc gctgtggatc
      481 gcgacctaga ggggaatcgg catcgtggaa tggaaggaat gggccctcag cctggcagtg
      541 ttcctgccgg cagtgggtgc ggtgatcgtg atgctgatcc cgcgcgcgca agaagaggcc
      601 atcaagtggg tgacgttgat cgcgaccgtt gccacgctcg gcgtcgtcat cggcatcctc
      661 gccgacttcg actacgacta cgtcacgccc gagggtggga gcgggctgca gttcgagctg
      721 aacaacaagt ggatcgaggt catcaacagc cgataccaca tcggcgtcga cggcatctca
      781 ctcccgctgc tcgctctgtc cgcgttcatc accgtcctgt gcgtcgtcta ctcgtggaac
      841 cacttccccg agccacacaa ccccaaggcg ttcctcgcac tcatgctgat cctcgaggtc
      901 ggcatgaacg gcacgttcgt cgcccaggac ctcatcctct tcttcatctt cttcgagatc
      961 gtcctgctcc cgatgttctt catgatcggg gtatggggcg gcccgaatcg tgagtacgcg
     1021 tcgatcaagt tcttcctgtt cacgctgttc ggttccgcgc tgatgttgct gagcttcctg
     1081 gcgctgtact tcaaggtcga cacggatatc gggcacacct tcgacatggt cgcattgacc
     1141 gatgccgcgg gcagtggtgt cgtgcacagc acgcagctgg tgatcttcgc gggactgttc
     1201 ctcgggttcg cgatcaaagt gccgatgttc ccgttccaca cctggctccc cgacgcgcac
     1261 accgaagcgc cgacggtggg ttcggtgctg ctggccgcga tcctcctgaa gctcggtacg
     1321 tatggcttcg tgcgcatcgc gttcccgatg ttgcccgacg ccgcggagag ctgggcgccg
     1381 tggatcggcc tgctcgcagt catcggcatc atctacggcg cgctcgggtg tctcgcgcaa
     1441 agagacatga aacgcctcat cgcgttctcg tcggtcgcgc acatgggctt cgtcatgctc
     1501 gcgatcgcga cgatgaccac tgccggcacc aacgccgcca tcttcggcat ggtcgcccac
     1561 ggtctgatca ccggcatgtt gttcttcatc gccggttcgg tgcagcacaa cttcgagacg
     1621 cgcgagctct cgcgcctcgg cggcttgctg atccaggcac cgaagatggg atggatcctc
     1681 ggcttctgtg cgatggcgag cctcgggctg cccggtctcg ccgggttctg gggcgagttc
     1741 cccgcgctcc tgtcggcgtt cgaaccgggc cccggactct ccgacgagtt gttccgcacg
     1801 ttcatggtca tcggtgcgat cggcaccgtg ctcgccgcgg gctacctcct gtggatgttg
     1861 cagaaggtcg cgttcggcac gccgaagccg gagttcgcgg acgcgcacat ccacgacgtc
     1921 aaccccttcg agtgggcggc gtggctcccg atcctgctgt tgatcctcgt gctcggcatc
     1981 tacccgaact tgttgttcaa ggtgaccgac cagacggttt cgcagggtcc gggccaaacg
     2041 accatcgtcg ccgaacaggc caacgaataa cgtgaacccg accttcgact ggcacgcgct
     2101 cgctccggag atcgtcgtct cggcgacgct tctcgtcgcg ctccttgccg acatgttctc
     2161 gaagcgccgc gacatcgtgc tcgtgtcgcg catcgcgtcg ttcggcgtgc tcggtgcgct
     2221 gatcccgatc atgacgctcg ccgcgagcga tcacgacacg cggtcgatgt tcggcggcgc
     2281 ctatgtcagc gacaactact ccttgacgct gcaggcgttc ttcctcgtcg cggcgtacgt
     2341 cacgatcctg ctgtcgatcg actacatcga cgagggcgac tactacaagg gcgagtactt
     2401 cttcctgctg ctctcgtcga cgctcgggat gatggtgatg gcgtccgcgc gtgacctcat
     2461 cacgatcttc gtcgcgctgg agctgatctc gatcccgacc ttcgtgctcg ccgcgttccg
     2521 caagcacgac cgccggtcga acgaggcggg cgtgaagtac tacctgatcg gcgtgctctc
     2581 gagcgcaatc atgctgtacg gcatgtcgct gatcttcggc ctctacggca ccacggtgct
     2641 gtcgcagatc aaccagcaag tgagcgcgga gggtgccgag ccactgctgt cggtcgcgat
     2701 cttcctcgca ctggtggggt tcgcgttcaa ggtgagcgcg gtgccgttcc acttctgggc
     2761 gcccgacacc tatgagggtg caccgacgcc ggtcaccgcg ttcctgtcgg tgtcgtcgaa
     2821 ggcgggcggc ttcgtcgccc tgctcagcat catcttcttc ggcttctacg gcgtcgacgg
     2881 cgcgggcagc gacctgtggt ggggcgcggt ctgggtcctc gcggtgctgt cgatgacgct
     2941 cggcaacctg gcggccctcc gccagacgaa catcatccga atgctcgcgt actcgtcggt
     3001 cgctcagggc ggcttcatac tggtgccgtt cgcggcggcg ggtattgcgg gcgccgacgg
     3061 caacaccgac gtcgccgcga ccgcgttcag cgcagtcgtc atctacttgc tgatctacgg
     3121 cgcgatgaac ctcggagcgt tcgcagtcgt catcgcggcg gcgcgccgga cgcgcagcgc
     3181 cgaggtctcg tcgttcgccg gcttgttcac gacgtcgccc gtgctgtcgg tgctcatgac
     3241 gatcttcatg gcctcgttgg ccgggatccc gccgctcgcg ggctggttcg ccaagttcgt
     3301 gatgttccgc tccacgctcg aagccggtac cacctgggcg gtcgtgctcg cggtgatcgc
     3361 ggcggtgaac tcggtgatcg cgttcttcta ctacgcgcgt gtcatccggg tgatgtggtt
     3421 ccacgagccc gccgaaggtg ccggtccggt gcaggtgccg ggcgcgctgg ccgcggcgct
     3481 cgcgatcacg accgcggtca cggtcgtcgt cggcatctac ccgcaggtgt tcgcacgcgt
     3541 cggagagctc gcattctgag gtgagcgcgg cggaggagat cgcgcggcgc atccgccgtg
     3601 agggtccgat cccgttcgag acgttcatgg agcacgcgct ctacgaaccc gatggtggct
     3661 tcttcacgtc gggcggcggc gccggtcggg ccggccgcga cttcatcacg tcacccgaag
     3721 tcggctcgtt gtacggcgcc tgcgtggccg agagcctcac gcgcggttgg cgcgagatgg
     3781 gcgagcccga tccgttcgtc gtcgtggagg cgggcgccgg caacgggcgg ctcgcggccg
     3841 acgtgctgcg cgtgcagccc gacttcgcat cggcgctgcg ctacgtgctc gtcgaacgat
     3901 cgccccggtt gcgcgacgag caacgcgcgc gccttccgct cgagccgcgc gacgaagccc
     3961 tcggcccgtt cgtcgagcgc gacgatcgcg ccgaaccgtt gccgcgccgg ggtcccatcg
     4021 tcgcgtcgat ccccgacctg cccgcgatca cgttcgaagg catcgtgttc gccaacgaat
     4081 tgctcgacaa cctgccgttc gggatcgcgg cattcgatgg cacccgctgg aacgaggtcc
     4141 gggtgaccgt cgacggcgac tgcttcgtgg aagtgctcgt tccgttggtc gacgaccatg
     4201 cgttgccgga tgtcggcgcg ggcgcgcgcg tgccgatccc gcacgcgttg acggcgtggt
     4261 tcgccgacct ggcgactgtg ttgcgccgcg ggtacgtgtt gctcgtcgac tacatcgtca
     4321 cgatggacga gctcctcgcg cgcggcgacg actggttgcg cacctaccgc gggcacggat
     4381 cggggaatga cccgttgtcg gaaccgggca gctccgacat cacgggcgat gtggtgcgcg
     4441 agcagctgtt gcgggcggcc gcgaccgcgg gcttcacgct cgtcgccgag cacacgcagg
     4501 ccgactggtt gcggggcctc ggcatcgacg cgctcgtcga cgaagggcgc cgggcatggg
     4561 aagaaggcgc tgctcgcggc gacctcgcag cggtcgccgg ccgcagccgg gtcaacgagg
     4621 ccgcggcgct gaccgacccc gccggcctcg gcgctcatcg cgtcgtcctg ctggccagat
     4681 ctgctcagtc gcccggtttc gacaggatga caagctttct gagctactga gcagatcttc
     4741 ggccgacagc gagcgcagcg agcggtgcgt cggacgaaac agtgtggacg tcggcgtagc
     4801 cggcctcggg gtccgggggc ggagccccga tagattccgc gtcatgaccg agacgctcgc
     4861 cgcgctgttg caagagggcc gcaccttccc gccgtcgccc gagttccgca aggacgcgct
     4921 cgtcaccgaa gccaaggtgt acgacgagtc cgaacgcgac tggcagggct tctgggccaa
     4981 gcaggccctc cagctcgact ggtcgaagga atggcacacc atcctcgaat gggatctgcc
     5041 gttcgcgaag tggttcgtcg gcggcgagct gaacgtcgcg cacaactgcc tcgatcgcca
     5101 cgtggcggcc ggccacggcg accagatcgc gtactactgg gagggcgagc ccggcgacac
     5161 gcgcgaggtc acgtaccagc agttgctcga cgagtcgagc cgcgtcgcga atgtgttgcg
     5221 gtcgctcggt gtcgccaagg gcgaccgcgt cgcgatctac atgggcatgg tgcccgaggc
     5281 cgcggccgcg atgttggcgt gcgcgcgcat cggcgcgccg cactcggtcg tgttcggcgg
     5341 gttcaccgcg gcttcactgc gcgaccgcat catcgacgcg gaagcgaagg tgctcatcac
     5401 cggcgacggc gcgtggcggc gaggtggcgt cgtgccgctg aaggacatcg ccgacgaggc
     5461 aatcgcggag acgccgagca tcgagcatgt gctcgtcttg cgccgcaccg agaacgacgt
     5521 cgcgatgaag gacggccgcg acatctggtg gcacgacgcg gttcccgccg cgtcgaccga
     5581 ttgccccgtg gaaccgatgg acagtgaaga cctgctgtac atcctctaca cctcgggcac
     5641 cacggggaag cccaagggca tcatgcacac gacgggcggt tacctcacgc agtgcgcgta
     5701 cacgcacaag tacgtgttcg acctgcaccc cgacaccgac atcttctggt gcacggccga
     5761 cgtcggttgg gtcaccggtc actcgtacat cgtctacggg ccgctcgcga accgcgccac
     5821 gagcgtcatg tacgaaggca cgcccgacta tcccgacaag gatcgcttct ggtcgatcat
     5881 cgcgaagtac aaggtcacga agttctacac ggcccccacc gcgatccgga cgttcatgaa
     5941 gtggggcgac gactatcccg cgcgccacga catgtcgtcg ttgcagctgc tcggcagcgt
     6001 cggcgagccg atcaatcccg aagcctgggt ctggtacttc aagacgatcg gcgacgaacg
     6061 ttgcccggtc gtcgacacgt ggtggcagac cgagacgggc gccatcatga tcagcccctt
     6121 gccgggggcc acgacgttga agccggggag cgcgacgttc ccgctaccgg gcatcaaggc
     6181 cgagatctgt gacgacgacg gcaaggccgt cggtatcccg ggcggcggct atctcgtgtt
     6241 gacgcgccca tggccggcga tgttgcgcgg catctggggt gatccgcagc gctaccgcga
     6301 cacgtactgg agccggttca gtgaccgtta cttcgcgggc gatggcgcca agcgcgacga
     6361 ggacggctac ttctggctcc tcggtcgggt cgacgacatc atgctcgtgt cgggacacaa
     6421 catctcgacc accgaagtcg agcacgcgct ggtcgaccac gaatcggttg ccgaagccgc
     6481 ggtcgtcggc aaggtcgacg agacgacggg ccaggcgatc gccgcgttcg tcatcctgcg
     6541 cgccggtttc gagccatccg acgcgctcgt cgaagagctg cgcgaccacg tcgcgaagct
     6601 catcggtccg atcgcgaagc cgaagtcggt cctgttcacc gaagagctgc cgaagacccg
     6661 ctcgggcaag atcatgcgcc gcttgttgcg caacgtcgcc gaagacgaag cgctcggcga
     6721 caccacgacg ctcgccgacc ccgcgatcgt cgacggcatc aagacgaaat acctcgcgac
     6781 gcaacccagt gaggagtgag tgacccggta cctcccggag cactggacct ggcacaacgg
     6841 tccggtggaa cccggccgcg ccgtcgtcgt cgacatcgac ggcgtgctct cggacgccac
     6901 cgagcgccag cactacatcg aggcgccgcg ggccaactgg cgggcgttct tcgacgcgtg
     6961 cggtgacgac ccggtcatcg aggaggtccg cacgctgctc gacctcctcg atcccgatct
     7021 tcaagtggtg ctgctcaccg cccgtcccca ccgcctccac gacctgacgg aggcgtggct
     7081 ccaccgctac gagatccgct ggaacctgct gatcatgcgg ccgtggggcg actacgagct
     7141 gtcgcgcgac ttcaagcagt cgacggtctg ggagcttcgc tcggccgggt tcgccctggc
     7201 cctggccttc gaggacgacc gccgcaacgt cgagatgttc cgcgccgaag gcatcccctg
     7261 cctctacgtc cacagcggct actacgattg atctttgtcg ctttgggctc gcgcgcgtgt
     7321 gcgacttcgg tgccttgccg agcccaaaga ctccgcgagt ggccgagacg gtcgtggggg
     7381 cgctgcgcgc cgtggggacg tgaggccacc gcccttccgg cgtctcgcgc cgggcctcgg
     7441 ggggcgctgc gcgccgcggg aggaccgaac atcgcgggct tgtagccacg tcctcacgtc
     7501 cgactagtcc cgaccgggcg aaattcacca ttacccccgg cccggacgcg ccgaaagaga
     7561 acgcaggagt tcgaggcgcg ggaagggatt cggccatgcc ggcgacgggc gggcgagcgc
     7621 gagcgatgat cggggcgcag ccatgcgcct gacccgcacg cgcaaggagg cgctcgacga
     7681 cttcggccag gccatcgcgg agcgcttcgc ggagccggcc gaggccgcgc cgggcg
//
LOCUS       DASUGA010000216         2170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1075404, whole genome shotgun sequence.
ACCESSION   DASUGA010000216 DASUGA010000000
VERSION     DASUGA010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075404"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VFX21_04265"
     CDS             complement(<1..516)
                     /locus_tag="VFX21_04265"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX5095198.1"
                     /translation="MSRPAAKSTSPQRTDLTTGERLQKVLARAGIASRRASEELIAAG
                     RVTVNGEIAQLGRRVDPATDKLAIDGVPVVVDATIVHWVLNKPAGYVTTAQDPHGRPT
                     VLDLVPDEPRVFPVGRLDLDTEGLLLLTNDGELAQLLTHPRHGVEKEYVAEVEGRPAP
                     AAIRALREGVEL"
     gene            complement(473..1108)
                     /gene="scpB"
                     /locus_tag="VFX21_04270"
     CDS             complement(473..1108)
                     /gene="scpB"
                     /locus_tag="VFX21_04270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX5095199.1"
                     /translation="MSDPEARRAVEAIVLAATESVHPTVIAQLLEMSVDAVDDLCNEL
                     AAEYETQGRGFQLARVAGGYRFQTHPEAYPYVERFVLEGQTVRLSGPALETLAIIAYK
                     QPVSRAQLSAIRGVNVDATLKTLVGRGYVEEIGHDQTPGNPALFSTTRLFLERLGLDS
                     LDELPALADFVPDASIVEALERGLRAPTNEEIAEEVAAVEAGGEIDLTAED"
     gene            complement(1105..1944)
                     /locus_tag="VFX21_04275"
     CDS             complement(1105..1944)
                     /locus_tag="VFX21_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX5095200.1"
                     /translation="MGYEVTTPVFEGPFDLLLHLILKEEVDLWEIKLERIVDAFVAEL
                     EKMERLDLEVTTEFLLIAATLVDLKSRRLLPGREDVDLDEELMRFEERDLLLARLLEC
                     KTFQDAAKVLHEQLVRGSQTFARTVGPEEPFRSLAPDPLERLPLDALVKAARKGLAPK
                     VETPTEVDTYHVAPIRASVRDAIETVLALLPERGAVQFRELTYGVQEKLEVIVRFLAV
                     LELFKQGIIDLEQVESFSELTVRPLAAGERVALDLSSLEEWGDDEVTPLQQVEAQTVE
                     EQV"
     gene            2046..>2170
                     /locus_tag="VFX21_04280"
     CDS             2046..>2170
                     /locus_tag="VFX21_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006656563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX5095201.1"
                     /translation="MARVFSGIQPTGEMHLGNYLGAVRRWVDSQPPAGSPEAAAH"
BASE COUNT          346 a          733 c          712 g          379 t
ORIGIN      
        1 gagctcgacg ccctcgcgca gggcgcgaat tgcggccggc gcgggccggc cctccacctc
       61 ggcgacgtat tccttttcga caccatggcg cggatgcgtc aacaactggg ccagctcacc
      121 gtcgttcgtg agcagcagca atccttcggt gtcgaggtcg aggcggccga ccggaaagac
      181 acgcggttca tccggcacga ggtcgaggac ggtcggccgg ccgtgtggat cctgtgcggt
      241 cgtcacgtag cccgcgggct tgttcaacac ccaatggacg atcgtggcgt cgacgacgac
      301 ggggacgccg tcgatcgcga gcttgtcggt cgcgggatcg actcgccgcc cgagctgcgc
      361 gatctcaccg ttgaccgtga cccgtccggc cgcgatcaat tcctcggacg cccgccgcga
      421 cgcaatcccc gcgcgcgcca gcaccttttg caggcgctcg cctgtggtca ggtcagtcct
      481 ctgcggtgag gtcgatttcg ccgccggcct cgacagccgc gacttcttcg gcgatctctt
      541 cgttcgtcgg cgctcgcagg ccgcgctcca gcgcttcgac gatcgacgcg tccggcacga
      601 agtcggcgag cgcgggcaac tcgtcgagcg agtccaggcc gagccgctcc aggaacagtc
      661 gcgtcgtcga gaacagcgcc ggattgccgg gcgtttgatc gtgaccgatc tcttcgacat
      721 agccgcgccc gacgagcgtc ttcagtgtcg cgtcgacgtt cacgccgcga atggccgaaa
      781 gctgtgcgcg cgacaccggc tgcttgtagg cgatgatcgc cagcgtttcc agggccggtc
      841 ccgagagccg cacggtttgg ccttcgagca cgaaccgttc gacgtacggg tatgcctcgg
      901 gatgggtctg gaaccggtac ccgcccgcga cgcgtgcgag ttggaagccg cggccttgcg
      961 tttcgtactc ggcggcaagc tcgttgcaga ggtcgtcgac ggcgtcgacc gacatctcca
     1021 acagctgcgc gatcaccgtg gggtgcacgg actcggttgc cgccagcacg atcgcttcga
     1081 ctgcgcgccg cgcttcggga tcgctcacac ctgctcctcg actgtttgcg cttcgacctg
     1141 ctggaggggc gtcacttcgt cgtcgcccca ctcctcgagc gaggacaggt cgagcgccac
     1201 ccgttcgccg gctgccaacg ggcgcaccgt gagttcgctg aagctctcga cctgttcgag
     1261 atcgatgatg ccctgcttga agagttcaag caccgcgagg aaccgcacga tgacttcgag
     1321 tttctcctgc acgccgtagg tgagttcacg gaactggact gcgcctcgtt cgggcagcaa
     1381 cgcgagcacc gtttcgattg cgtcgcgcac gctcgcgcgg atcggcgcga cgtgataggt
     1441 gtcgacctcg gtcggcgttt cgaccttcgg cgcgagtccc ttgcgcgcgg ccttgacgag
     1501 cgcgtcgagc ggcagccgtt cgagcggatc gggcgcgagc gaacggaacg gctcttcggg
     1561 gccaacggtg cgcgcgaacg tctggctgcc ccgcacgagt tgttcgtgca gcaccttcgc
     1621 cgcgtcctgg aacgtcttgc actcgagcag ccgcgccaac agcaagtcgc gctcttcgaa
     1681 gcgcatgagt tcttcgtcga ggtcgacgtc ctcgcggccg ggaagcaacc ggcgagactt
     1741 caggtcgacg agcgtcgccg cgatcaacag gaattcggtg gtcacctcga gatcgaggcg
     1801 ttccatcttc tcgagctcgg ccacgaacgc gtcgacgatg cgctcgagct tgatctccca
     1861 gagatcgacc tcttccttca ggatgaggtg gagcagcagg tcgaacgggc cctcgaagac
     1921 gggagtcgtg acctcgtagc ccatgcgccg cccagattat cgggatcgcg cgcgaatcac
     1981 agcgatgtca tacacccccg aagaacaggg aaaatgccgg cgcgaccggt atcgtctcgg
     2041 cccgtatggc acgagtgttt tcgggcatcc agcccaccgg cgagatgcac ctgggcaact
     2101 acctcggcgc agtccggcgc tgggtggaca gccagccacc cgcgggctcc cccgaggcgg
     2161 ccgcccacga
//
LOCUS       DASUGA010000217         3509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_177519, whole genome shotgun sequence.
ACCESSION   DASUGA010000217 DASUGA010000000
VERSION     DASUGA010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177519"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1767
                     /locus_tag="VFX21_04285"
     CDS             172..1767
                     /locus_tag="VFX21_04285"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5095202.1"
                     /translation="MDSCDVLIVGGGITGIYQLYRAREVGFSVRLLEAGGGVGGTWYW
                     NRYPGARFDSESYTYGYLFSRELFDEWEWTEHFAEQPETERYLNHVVDRFDLRRDIVC
                     NARVTSAVYDEASCAWTVTTSDGSSWRAQFLVAATGVLSVPYFPDVPGRDDFAGEQYH
                     TGLWPSAPVDLRNKRVAVIGTGSSGVQVIPAIADEVASLTVYQRSANWCTPLNNAPVT
                     PEEQAALRADFESIRETLNTSLHGFLHPACDRGTFDDDAEQRQAFYEEMWNSRGFSKL
                     TSNYTDMTVDESANAEWREFVAAKVRSIVADPATAEKLIPKDHRFGEKRPPFVTGYYE
                     TYNKPHVSLVSLAETPITRITGAGIETADGLQEFDVIIWATGFDFGTGALNRMGIRGR
                     DGRALEDHWADGPETFLGIQTTGFPNFFFPGGPHAAAGNNPRYNGDQVDFVTDTLVYV
                     RDHGFHAIEVNADAEERWTSMVDTASHRPPSFGEGSYYFGTNIPGKPKRYLLNAGGRP
                     KLFKEIARVKANDYAAFTLSRRA"
     gene            complement(1745..2806)
                     /locus_tag="VFX21_04290"
     CDS             complement(1745..2806)
                     /locus_tag="VFX21_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX5095203.1"
                     /translation="MAKRWQQRPPGSTWGDWGDDDELGRINLLTPEKVLQGVREVEAG
                     ISFCLSLPLDYPGGTSLNQRRYPPRIAPTEDLDGAPEVFYNVRQRDIPKYGSPLHVDV
                     WADDQVTLWLQYSTQWDSLAHAGAEFDVDGNGDEVSVFYNGYRAGVDLVGPKEDARGD
                     GGDNRSFAHHLGLEHMAFHGVQGRGVLIDVAHHLGHEWRGVNYEMLQEIMQADDVVVE
                     PGDMVLFHTGFATKVLEWNREPDPVEIHRTCAYLDAHDPALLEWIADSQLSALVADNY
                     AVEGMVGRRNPDPERHSLLPIHHLCLFKLGVPLGEMWYLHELATWLREHHRSRFLLTA
                     PPLRLPGIVGSPLTPVATV"
     gene            2868..>3509
                     /locus_tag="VFX21_04295"
     CDS             2868..>3509
                     /locus_tag="VFX21_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013426443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5095204.1"
                     /translation="MPDKDFVVSADGHLLEPIDLFKQRLPEHLRDFAVWEEEIEIEPF
                     VEGGARVFRKLHTPGFEGWTVSRYRQTSGRTPEGDPEIILEDMDADGVDAQVLFPNLS
                     QFGLFTDRHDTSIAHARVYNDYVAERFSPYFHRLAPTAPIPLTDVDDAVAEVERVANA
                     GFRAVTLPATAPRPYYTADFDPVWAAIQGSGMNVFFHTQTGGVKVDDPEALTLR"
BASE COUNT          590 a         1170 c         1177 g          572 t
ORIGIN      
        1 cgagccggca cgccatctgc cagatccgcg gccacaactg ctcggcttcc cgcgcgtaga
       61 agtccgggtc gtagtagcgc tccttgggaa cgcggtccgg ggtgtccatg gcgtagggca
      121 cgggcacagc cggacggtat acgaaccggt cggtacaatg ccgcccgcct catggactcc
      181 tgcgacgtat taattgtcgg cggcggcatc accggcatct atcagctgta ccgcgcacgg
      241 gaggtcggct tctcggtccg cctgctcgag gccggcggcg gcgtcggcgg cacgtggtac
      301 tggaaccgct accccggtgc gcggttcgac tccgagagct acacctacgg gtacctgttc
      361 tcacgcgagc tgttcgacga gtgggagtgg accgagcact tcgccgagca gcccgagacc
      421 gagcgttatc tgaaccacgt cgtcgaccgc ttcgacttgc gtcgcgacat cgtctgcaat
      481 gcgcgggtca cgtcggcggt gtacgacgag gcgtcgtgcg cgtggacagt aacgacgtcc
      541 gacggatcgt cgtggcgcgc gcagttcctc gtcgcggcga ccggcgtgct gtccgtgccg
      601 tacttccccg atgtgccggg acgtgacgac ttcgccggtg agcagtacca caccggattg
      661 tggccgtcgg cgccggtcga tctgcgcaac aaacgcgttg cggtgatcgg tacgggatcg
      721 agcggtgtgc aagtgattcc cgcgatcgcc gacgaggtcg catcgctgac cgtgtaccaa
      781 cgcagcgcga actggtgcac gccgctgaac aatgcgccgg tcacgccgga ggagcaggcg
      841 gcgctgcggg ccgacttcga gtcgatccgc gagacgctga acacgtcact gcacgggttc
      901 ctgcatcccg cgtgcgaccg cggcacgttc gacgacgacg cggagcaacg gcaggcgttc
      961 tacgaggaga tgtggaacag ccgcgggttc tcgaagctga cgagcaacta caccgacatg
     1021 acggtcgacg agtctgcgaa cgccgagtgg cgtgagttcg tcgcggccaa ggttcggagc
     1081 atcgtcgccg acccggcaac ggcggagaag ctcatcccga aggaccaccg cttcggtgag
     1141 aaacggccgc cgttcgtcac cggctattac gagacctaca acaagccgca cgtctcgttg
     1201 gtctcactgg ccgagacgcc gatcacccgc atcaccggcg ccggcatcga gaccgcggac
     1261 gggttgcagg agttcgacgt catcatctgg gcgaccggtt tcgacttcgg caccggcgcg
     1321 ctgaaccgca tgggcatccg tggtcgcgac ggacgcgcgc tcgaagacca ctgggccgac
     1381 ggaccggaga cgttcctcgg tatccagacc accggcttcc cgaacttctt cttcccgggc
     1441 ggaccgcacg cggcggccgg caacaacccg cgttacaacg gcgaccaagt cgacttcgtc
     1501 acggacacac tcgtctacgt ccgcgaccac ggcttccatg caattgaggt gaacgcggac
     1561 gcggaggagc gatggaccag catggtcgac accgcgtcac accggccacc ctcgttcggt
     1621 gagggcagct actacttcgg tacgaacatc cccggaaagc cgaagcgcta cctgttgaac
     1681 gcgggtggcc gcccgaagtt gttcaaggag atcgcgcgcg tgaaggcgaa cgactacgcc
     1741 gcgttcacac tgtcgcgacg ggcgtgagcg gcgagcccac gatgcccggc aggcgcagcg
     1801 gaggcgcggt gagcaggaag cggctgcggt ggtgctcgcg cagccacgtc gccagctcgt
     1861 gcaggtacca catctcgccg agcgggacgc ccagcttgaa caggcacagg tggtggatgg
     1921 gcagcagcga gtgccgttcg gggtccgggt tgcggcggcc gaccatgccc tcgaccgcgt
     1981 agttgtcggc gacgagcgca gagagctggg agtccgcgat ccactcgagc agggccgggt
     2041 cgtgggcgtc gaggtacgca cacgtgcggt ggatctcgac gggatcgggt tcgcggttcc
     2101 actcgagcac cttggtcgcg aagccggtgt ggaacagcac catgtcgccg ggctccacga
     2161 cgacgtcgtc ggcctgcatg atctcctgga gcatctcgta gttcacaccg cgccactcgt
     2221 gcccgagatg gtgcgcgacg tcgatcaaca cgccgcggcc ctgcacgccg tggaacgcca
     2281 tgtgttcgag gccgagatgg tgcgcgaacg aacggttgtc accgccgtcg ccgcgcgcgt
     2341 cttccttcgg tccgaccaga tcgacgcccg cgcggtaccc gttgtagaac acggagactt
     2401 cgtcgccgtt accgtcgacg tcgaactccg cacccgcgtg cgcgagcgag tcccattgcg
     2461 tcgagtactg caaccagagc gtcacttggt cgtcggccca tacgtcgacg tgcagcgggc
     2521 tgccgtactt ggggatgtcg cgctggcgga cgttgtagaa gacctcgggc gcgccgtcga
     2581 ggtcttcggt cggcgcgatg cgcggcgggt aacgccgctg gttcaacgac gtgccgcccg
     2641 gatagtcgag gggcaggctc aagcagaagc tgatgccggc ctcgacctcg cgcacaccct
     2701 gcaagacctt ctcgggtgtg agcaggttga tacgcccgag ctcgtcgtcg tcgccccaat
     2761 cgccccaggt cgaaccgggc gggcgttgct gccaccgctt ggccacggcg gttgtctagc
     2821 accgcgtcgc gttgcctgta ccgaccggtt ggtttaccct cgaaccgatg ccggacaagg
     2881 acttcgtcgt ctccgcggac ggtcacctcc tcgagcccat cgacctgttc aagcagcgcc
     2941 tccccgagca cttgcgcgac ttcgcggtgt gggaggagga gatcgagatc gagccgttcg
     3001 tcgagggcgg cgcgcgcgtg ttccgcaagt tgcacacgcc gggcttcgag ggctggacgg
     3061 tgtcgcgcta ccgccagacg agcgggcgca cacccgaggg cgatcccgag atcatcctcg
     3121 aggacatgga cgccgacggt gtcgacgcgc aggtgctgtt cccgaacctc tcgcagttcg
     3181 gcctcttcac cgaccggcac gacacatcga tcgcgcacgc tcgcgtctac aacgactatg
     3241 tcgccgaacg gttctcgccg tacttccatc ggttggcgcc gaccgcgccg atcccgttga
     3301 ccgacgtcga cgacgcggtc gccgaggtcg aacgggtggc caacgccggc ttccgcgcgg
     3361 tgacgttgcc ggcgaccgcg ccgcgcccgt actacaccgc cgacttcgat ccggtgtggg
     3421 cggccatcca gggttccggg atgaacgtct tcttccacac ccaaaccggc ggcgtgaagg
     3481 tcgacgatcc cgaagcgctc accctccgg
//
LOCUS       DASUGA010000218         2002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1490944, whole genome shotgun sequence.
ACCESSION   DASUGA010000218 DASUGA010000000
VERSION     DASUGA010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490944"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VFX21_04300"
     CDS             complement(<1..807)
                     /locus_tag="VFX21_04300"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5095205.1"
                     /translation="MAVTRDDAPVRLAELVASLSLGIDLGFGQPMEHVLRQCLVGLRL
                     GEQIGLDESDRATVYYTALLVNVGCHSDAHEQAKWFGDDIAMKSTKYEYEPFTVADAV
                     AMVRLLGSGGTPLHRFRTGLEFAIGGFKEVNTMIADHAALARELGVQLGLPPSVLDAL
                     GASYERWDGRGWPGKLSGDEIPMASRVAQLAEFVEVAYRVGGIDAAIEVARKRRGTQF
                     DPLLVDALVLDAPKIFFHLDDVASWDAVIDAEPSLAVALTDDECDDALRAI"
     gene            945..1328
                     /locus_tag="VFX21_04305"
     CDS             945..1328
                     /locus_tag="VFX21_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019985448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5095206.1"
                     /translation="MAKATRATTPITMDVELAEDRSCALDDYTVNFVTIRQSHDLAPM
                     LAVLPDGLCHCPHWGVITTGRMTVTYPDREEVYEAGDAFYMTPGHTPAAEAGTEFVMF
                     SPTDLLEETEAAIQEGMQEAAAQTL"
     gene            complement(1357..>2002)
                     /locus_tag="VFX21_04310"
     CDS             complement(1357..>2002)
                     /locus_tag="VFX21_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5095207.1"
                     /translation="VLRTIVQTDLRTGSFGASLAALGFRPEDITDVLLSHLHFDHIGW
                     LSVDGRPYFPNAVVRCEQHDAEYFLAPDFDDGFYRVLWNAMPTSERMAPVLAQFEPWA
                     EDAAIAPGLDCVYAPGHTPGSAIFSITSGSERALILGDSVHCPQELMDPDFFGGGVDI
                     AQADATRERIRRDAEDGATLLSAPHFPRLQFGRLRVGDRPRRWSFEWVPPTSAA"
BASE COUNT          341 a          697 c          689 g          275 t
ORIGIN      
        1 gatggcgcgc agcgcgtcgt cacactcgtc atcggtcagc gcgaccgcga gcgacggctc
       61 cgcatcgatc accgcgtccc acgacgcgac gtcgtcgagg tggaagaaga tcttcggcgc
      121 gtcgagcacg agcgcgtcga ccagcagcgg gtcgaactgc gtcccgcgac gcttgcgggc
      181 aacctcgatc gcggcgtcga tgccgccgac ccggtatgcg acctccacga actcggcgag
      241 ctgcgcgacg cgcgacgcca tcgggatctc gtcgcccgag agcttgcccg gccagccgcg
      301 accatcccac cgttcgtacg acgcgccgag ggcgtcgagc acgctcggcg gcagaccgag
      361 ctgcacaccg agctctcggg cgagcgcggc gtggtcggcg atcatcgtgt tcacttcctt
      421 gaagccaccg atcgcgaact cgaggcccgt ccggaaccgg tgcagcggcg tgccgcccga
      481 accgaggagc cgcaccatcg ccaccgcgtc ggccacggtg aacggctcgt actcgtactt
      541 cgtcgacttc atggcgatgt cgtcgccgaa ccatttcgcc tgctcgtgcg cgtcggagtg
      601 gcagccgacg ttcacgagca gcgccgtgta atacacggtc gcgcggtcgg actcgtcgag
      661 accgatctgc tctccgagtc gcagcccgac gaggcactgg cgcaggacat gctccatcgg
      721 ctgaccgaat ccgagatcga tcccgagtga cagcgacgcg acgagctcgg ccagacgtac
      781 cggcgcgtcg tcgcgcgtga ccgccattcc gccgagcgta agccaccgcg cggaaaccca
      841 ccaggaatgg ggtgttcgcc tcatgacgcc gtcggcgggt ctccgtaccg tgcgaggaag
      901 cctcccgccg ggagcgcagg accagccgac gacaggagca accgatggcg aaggcaacca
      961 gggccacgac cccgatcacg atggacgtcg agctcgccga ggaccgctcg tgcgcgctcg
     1021 acgactacac ggtgaacttc gtgacgatcc gtcagtcgca cgatctcgcg ccgatgctcg
     1081 cggtgctccc cgatgggctg tgccactgtc cgcactgggg cgtcatcacg accggtcgga
     1141 tgacggtgac ctatcccgac cgcgaagagg tgtacgaggc gggcgacgcg ttctacatga
     1201 cgcccgggca cactccggcg gccgaggccg gcaccgagtt cgtgatgttc agcccgacgg
     1261 acctgctcga ggagaccgaa gccgcgatcc aggagggcat gcaggaggcg gcggcccaga
     1321 cgctctgatc aggaccgggt gatcgccacc gggtgatcac gcggccgacg tcggcggcac
     1381 ccactcgaag ctccagcgcc gcgggcgatc accgacgcgc agccgtccga actgcagtcg
     1441 cgggaagtgc ggcgccgaca gcaacgtggc gccgtcttcc gcgtcgcgac ggatgcgctc
     1501 gcgcgtcgcg tcggcctggg cgatgtccac gccaccgccg aagaaatcgg ggtccatcag
     1561 ctcctgcggg cagtgcacgc tgtcgccgag gatgagcgca cgctccgaac cggacgtgat
     1621 cgagaagatc gcgctccccg gggtgtggcc gggtgcatac acgcagtcga ggcccggcgc
     1681 gatcgcggcg tcttcggccc acggctcgaa ctgcgcgagc acgggcgcca tccgctccga
     1741 cgtcggcatc gcgttccaca gcacgcggta gaagccgtcg tcgaagtcgg gtgcgaggaa
     1801 gtactccgcg tcgtgctgct cgcagcgaac gaccgcgttc gggaagtacg gccggccgtc
     1861 gacgctcagc catccgatgt gatcgaagtg caggtgcgag aggaggacgt cggtgatgtc
     1921 ctcgggccgg aacccgagcg cggcgaggct ggcgccgaac gagccggtgc ggagatccgt
     1981 ctgcacgatc gtgcgcagca cc
//
LOCUS       DASUGA010000219         3077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1021830, whole genome shotgun sequence.
ACCESSION   DASUGA010000219 DASUGA010000000
VERSION     DASUGA010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021830"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFX21_04315"
     CDS             <1..262
                     /locus_tag="VFX21_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095208.1"
                     /translation="VRQQATFACHKTTVAVEMEDGEEEMTVTDKSEHCAGAMIMLEHM
                     GRPNQMMRIAERLGFYEPTKLDMAAPVYKHGRDFIRAHRGNR"
     gene            283..456
                     /locus_tag="VFX21_04320"
     CDS             283..456
                     /locus_tag="VFX21_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095209.1"
                     /translation="MEQAESPERRRVRLEETVAYHERHGRVALAEGRHDDFRYHVARI
                     AMARMYLHRARLQ"
     gene            510..728
                     /locus_tag="VFX21_04325"
     CDS             510..728
                     /locus_tag="VFX21_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095210.1"
                     /translation="MHELEPGAKFKFDDSDEVYEVASHGDGTSFIRPVAVRTVKKTIT
                     PRFAEARTFEVQERAPRRCAPGAQVIPV"
     gene            725..910
                     /locus_tag="VFX21_04330"
     CDS             725..910
                     /locus_tag="VFX21_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095211.1"
                     /translation="MSASKLAEEVRTIEYVLRQGKCITYACGSQAQAAEIVAALLTRW
                     PSAPIYSITFKVLDEGG"
     gene            1163..1696
                     /locus_tag="VFX21_04335"
     CDS             1163..1696
                     /locus_tag="VFX21_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5095212.1"
                     /translation="MIWFTADTHFGHAAIMRYSERPFVDVDAMDEYLIATWNEHIKPT
                     DTVWHLGDFSLRAPDPYWARLNGHKNIIFGNHDDPKKLTRLAVAGGGVAKEVHNLRNG
                     RQRIFLSHYAHRTWPASHYGTLHLFGHSHGALSRWGRSMDVGVDAIGGYRPISLDEVI
                     EELRFVAPMDDVGRMVP"
     gene            1693..1878
                     /locus_tag="VFX21_04340"
     CDS             1693..1878
                     /locus_tag="VFX21_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095213.1"
                     /translation="MNRPNGNRAHDTALYVAGYRKLTDDELNTALVARNPSPSPWQRE
                     AIRKVLWERKVAKGREP"
     gene            1875..2339
                     /locus_tag="VFX21_04345"
     CDS             1875..2339
                     /locus_tag="VFX21_04345"
                     /inference="COORDINATES: protein motif:HMM:NF023101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger-containing protein"
                     /protein_id="HEX5095214.1"
                     /translation="MSVRDEDLACPDCAAPMELAIGYGGRQYYRCERYPDCHGAHGAH
                     PDGSPMGEPADARTRQARILAHEALDKLWMDAPLMYGAEALDPEKVKRFRRIARVRAY
                     DWLAAKMGGAYMHIGDADMQACRDIIAICNRATPEIVRAWAKSNADDQRRAR"
     gene            2311..>3077
                     /locus_tag="VFX21_04350"
     CDS             2311..>3077
                     /locus_tag="VFX21_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095215.1"
                     /translation="MPTINVAPGRVYWRHDAEDDGSGAPVWHRGTRWPDGRVELPSPR
                     ARGTIVVYHSPDEHDAFRRTVLHGPPSNSIIHFQGRIRARRQWVTVEDDRARDEQREQ
                     RRGDEPGAWRTFAEAFDEMGERARQATRETARLTQQRIRDHQDDALRYGSSTRRYTYE
                     DDVARLTLPEFTGDAVLRFYPQDEPTQWYTTDVAGNLIPIPEPPEEDEMPRGDQLSFF
                     TDPRYPPTFATIEVSQIELTREAPNPPFVENVQRLGIV"
BASE COUNT          597 a          950 c         1070 g          460 t
ORIGIN      
        1 cgtccgacag caagctacgt tcgcgtgtca caagacgacc gtggcggtgg agatggaaga
       61 tggtgaggag gaaatgacgg tgacggacaa gtcggagcac tgcgcgggcg cgatgataat
      121 gcttgagcac atgggccgac cgaaccagat gatgaggatt gcggagcggc tcgggttcta
      181 cgagccgacg aagctggaca tggcggcgcc cgtctacaaa catgggcgag acttcatccg
      241 tgcacacagg gggaaccgat gatcgcggac acgagcagga acgtggaaca ggccgagagc
      301 ccggagcgtc ggcgcgtgcg gctggaggag acggtcgctt accacgagcg gcacggccgg
      361 gtcgcgttgg ccgaggggcg gcacgacgac tttcgctacc acgtggcgcg catcgcgatg
      421 gcccgcatgt accttcaccg ggcgcgactc cagtgagccg gcggtgcgcg aagtgcctgc
      481 ggaccgggat gcacaacgac gtgcggcacg tgcacgagtt ggagccgggt gccaagttca
      541 agttcgatga cagcgacgaa gtgtacgaag tggcgtccca cggcgacggt accagcttca
      601 ttcggccggt tgccgtgcgc acggtcaaga agacgatcac gccgcgcttc gccgaggcgc
      661 ggacattcga ggtccaggaa cgtgccccgc gcagatgtgc gccgggtgca caggtgattc
      721 ccgtatgagc gcgagcaagc tggccgagga ggtgcgcacg attgagtacg tgctgcgaca
      781 gggcaagtgc atcacgtacg cgtgcgggtc gcaagcgcag gcggcggaga tcgtcgccgc
      841 gttgctcacg cggtggccgt ccgcgccaat ctattcgatc acgttcaagg tgctcgacga
      901 aggcggttga ccggcaacgg tagttgacgt aggttcgaac cgttcggaag caacggaaca
      961 tccacacaca gcaacgggag gcggtctgtc tgcggtacga cgtcatcatc cgcaaccggc
     1021 gtgaggcgtc cccggccgtg tcgttgctcc acagcatcgc gcaggacgac cggcagttcg
     1081 cgttgcaagg cagggtgtcg tggtcgcagc gcatggcgcg cggcacggac ccgatcgtga
     1141 cggcggttcg gagggtcaag ctatgatctg gttcacggcg gacactcact tcggtcacgc
     1201 ggcgatcatg cggtacagcg agcgtccgtt cgtagacgtg gacgccatgg acgaatattt
     1261 gatcgccacg tggaacgaac acatcaagcc taccgacacg gtgtggcacc ttggcgactt
     1321 ctcgctgcgt gcgcccgacc cgtactgggc acggttgaac ggtcacaaaa acatcatctt
     1381 cgggaaccac gacgacccca agaagttgac gcggttggcg gtggccggcg gtggcgtcgc
     1441 caaggaagtg cacaacctac gtaacggccg gcagcgcatc ttcctgtcgc actacgcgca
     1501 ccgcacgtgg ccggcttcgc actacgggac gttgcatttg ttcggtcatt cgcacggcgc
     1561 gttgtcgcgt tggggtcggt ccatggacgt gggcgtcgac gcgatcggcg gttaccgccc
     1621 gatctcgcta gacgaggtga tcgaggagct tcgattcgtc gcaccgatgg acgacgtcgg
     1681 gaggatggta ccatgaaccg accgaacggc aaccgcgcgc acgacacggc gctgtatgtc
     1741 gccgggtacc gcaagctgac ggacgacgaa ctgaacaccg cgctggtggc gcgcaatccg
     1801 tcgccgtcgc cgtggcagcg cgaagcgatc cgcaaggtgt tgtgggaacg caaggtcgcg
     1861 aaggggcgcg agccatgagc gtgcgcgacg aagacctggc gtgccccgat tgcgcggcac
     1921 cgatggagtt ggcgatcggg tacggtgggc ggcagtacta ccgctgtgaa cggtacccgg
     1981 actgccacgg cgcgcacggt gcgcacccgg acgggtcacc gatgggcgag ccggccgacg
     2041 cgcgcacgcg tcaggcgcgc atactcgcgc acgaggcgct cgacaagctg tggatggacg
     2101 cgccgctgat gtacggtgcc gaggctctcg acccggagaa ggtgaagcgg ttcaggcgca
     2161 tcgcacgcgt gcgcgcgtac gactggctgg ccgcaaagat gggcggcgct tacatgcaca
     2221 tcggggacgc cgacatgcag gcgtgccgcg acatcatcgc catctgcaac cgcgcgacac
     2281 cggagatcgt gcgcgcatgg gcgaagtcga atgccgacga tcaacgtcgc gcccggtagg
     2341 gtctactggc ggcacgacgc cgaggacgac gggtcgggcg cgcccgtgtg gcaccgaggg
     2401 acgcgctggc ctgacggtcg ggtggagtta ccgtcgcccc gcgcgcgggg caccatcgtc
     2461 gtttaccact cgccagacga gcacgacgca ttccggcgca cggtgctgca cggcccgccc
     2521 tcgaactcga tcatccactt ccagggccgc atccgggcac gacgccagtg ggtcacggtg
     2581 gaagacgacc gcgcgcgcga cgagcaacgt gagcagcgcc gtggcgacga gcccggtgcg
     2641 tggcgcacgt tcgccgaagc gttcgacgag atgggcgagc gtgcgcgcca agccacccgg
     2701 gaaacagcgc ggctgacgca gcaacgaata cgcgatcacc aagacgacgc gcttcggtac
     2761 gggtcgtcca cgcgtcgcta cacgtacgag gacgacgttg cccgtctcac gttgcccgag
     2821 ttcacgggcg acgcagttct acggttctac ccccaggacg aaccgactca gtggtacacg
     2881 actgatgtcg caggaaattt aatcccaatc cccgagccac ccgaggaaga cgaaatgcca
     2941 cgcggcgacc aattgagttt cttcaccgac ccgcgctacc cgccgacgtt cgcgaccatc
     3001 gaagtctcgc agatcgaact gacgcgcgag gcaccaaacc ctccgttcgt ggagaacgtc
     3061 cagcggctag gcatcgt
//
LOCUS       DASUGA010000220         3834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_520157, whole genome shotgun sequence.
ACCESSION   DASUGA010000220 DASUGA010000000
VERSION     DASUGA010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520157"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VFX21_04355"
     CDS             complement(<1..321)
                     /locus_tag="VFX21_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5095216.1"
                     /translation="MEAAARLARQKGPGGFSMDVLAKEAGVARATVYEHFRSKRVVLD
                     ELASSAARSVTLETSPSTETDPLRALRDTLSEVCRHWASHEETMRELQTLSAMTGGER
                     IGDAI"
     gene            complement(463..2007)
                     /locus_tag="VFX21_04360"
     CDS             complement(463..2007)
                     /locus_tag="VFX21_04360"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX5095217.1"
                     /translation="MRIAALDLGSNSFHLLVADVHPDGTFESITREKEMLRLGDEVSR
                     YGSIGFAAAERAVASVRRLTALAEAVGATEVLAKATSAIRTAENSSELVDRIEAETGV
                     EVEVISGLEEARLIFAAVRASVVIDPPPALCIDIGGGSVELVIGDSAGMRWAASVPLG
                     VGRLTEELVRSDPPTKSDRKRVEDRVREAFEPFVREVKERDPQMCVGTSGTINDLAAM
                     TEVCRTGEVPSLANALRVSRKDLLDLQERVLQLKTSERRRLPGLEEKRAELIPAGIVL
                     LTTALDIFGVDTLTVSDWALREGIVLDAVRTHDPDDWSDDPRALRRAAVANLARRCNS
                     DERHTRHVARLALELFDQTTELHGLDAHDREMLEFAALLHDIGQHVSRKGHQRHAAYL
                     VENAQLRGFDPGEIAFLSALVRHHRRGEPKRSEPRFGALTPAERTKVRKLAALLRVAD
                     GLDRGRRGVVERMTVDVGTDLVMLRLVTNGDAELALWGARRRRDLFEQVFERELECSV
                     LSAQNV"
     gene            2042..3502
                     /locus_tag="VFX21_04365"
     CDS             2042..3502
                     /locus_tag="VFX21_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013424450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5095218.1"
                     /translation="MDLTFESRMLIDGNLVEAGGAKTYDNVNPATEAVIGPVADGSVA
                     DMQRAIAAARRAFDETDWATNRSLRKECLTQLKEALDKHKEDLRPQIVAEVGTPIGLT
                     YAIQQDSCIDDMQWDIDQIDNYEWEHELGAHEFFGLRSNRLVSREAIGVVGLITPWNF
                     PFMLNLSKLAPALAAGCTAILKPAPDTPYSATWIGKLVAEETDIPAGVFNVVTSADPA
                     AVGEALTGDERVDMISFTGSTGVGKRIAARGADTLKRVFLELGGKSANIMLDDADFSG
                     LASAGMVCMHSGQGCAITTRLLLPRSRYDEGVHKVRESFESFAYGDPNDIANMAGPLI
                     NARQRERVLGYIEKGKAEGAKCLVGGGPATQFERGYFVQPTLFVDVDPSSTIAQEEIF
                     GPVLSVIPYEDDDDAVRIANASRYGLSGAVSSASLDRALAVARRIRTGTIAVNGGQWF
                     GPDSPFGGYKESGLGREHGVQGFEEYLETKTIGLPA"
     gene            3648..>3834
                     /locus_tag="VFX21_04370"
     CDS             3648..>3834
                     /locus_tag="VFX21_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEX5095219.1"
                     /translation="MLHRADHELFELAEDQDGIFTNHDADERGLTRPQGKRRIGSLWV
                     PMHRGVHRMAGAPLTQRG"
BASE COUNT          624 a         1320 c         1277 g          613 t
ORIGIN      
        1 gatcgcgtcg ccgatgcgtt cgccgcctgt catcgcgctc agcgtctgga gctcgcgcat
       61 cgtctcttcg tgcgacgccc agtgccggca cacctccgac aacgtgtcac gcaacgctcg
      121 cagcggatcg gtttcggtcg acggtgacgt ctcgagcgtg accgaacggg cggctgacga
      181 cgcgagctcg tccaacacga cgcgcttcga tcgaaagtgt tcgtaaacgg tcgcgcgcgc
      241 aactcctgct tccttggcca acacgtccat gctgaagcca cccggaccct tttggcgcgc
      301 caaccgtgcg gcggcctcca agatccgggt gcgcgtagcc cgtaccaact ctcgacgcgt
      361 aggactcaca ccacactccg aagcaatggg atttgcccgc cccacgcggt agacccgaaa
      421 tcctcgcgca cgaaacccgc gcgcttacgg tcggccttct gttcagacgt tctgcgcgct
      481 gaggaccgag cattcgagct cgcgctcgaa cacctgctcg aagagatcac gccgtcgccg
      541 cgcgccccat aacgcaagct cagcatcccc gttggtcacc aaacgcaaca tcacgagatc
      601 tgtacccaca tcgacggtca ttcgttccac gacgccacgg cggccccgat cgagcccgtc
      661 ggcaactctc agcagtgcag ccaatttgcg cactttggtc cgctcggcgg gcgtgagtgc
      721 accgaagcgc ggttcggacc gcttcggctc cccgcgccgg tggtggcgaa cgagggccga
      781 caagaaggcg atctcgcccg ggtcgaaacc gcgcagctgc gcgttctcga cgaggtacgc
      841 ggcgtggcgc tggtggccct tgcgggagac gtgctggccg atgtcgtgca gcaacgcggc
      901 gaactcgagc atctcccggt cgtgggcgtc gagtccgtgc aactcggtcg tctggtcgaa
      961 cagttcgagc gcgagacgcg caacgtgccg agtgtggcgc tcgtcggagt tgcagcgccg
     1021 cgcgaggttg gcgactgccg cgcggcggag ggcgcgcggg tcgtccgacc agtcgtcggg
     1081 atcgtgcgtg cggacggcgt cgagcacgat gccttcgcgc aacgcccagt cactgaccgt
     1141 cagtgtgtcg acaccgaaga tgtcgagcgc cgtcgtgagc aagacgatgc cggcggggat
     1201 gagctcggcc cgtttctctt cgaggcccgg gagccggcgt cgctccgacg tcttgagctg
     1261 caacacgcgt tcctggaggt cgagcaggtc cttgcgggac acccgcagcg cgttcgccaa
     1321 cgacggcacc tcgccggtgc ggcacacttc cgtcatcgcg gcgagatcgt tgatcgtgcc
     1381 gctcgtgccg acgcacatct gcggatcgcg ctctttgacc tcccgcacga acggctcgaa
     1441 cgcctcgcgc acgcggtcct cgacccgttt gcggtcggac ttcgtcggcg ggtcgctgcg
     1501 cacgagctct tcggtcaacc ggcccacacc gagcggcacg ctcgcggccc agcgcatccc
     1561 cgcgctgtcg ccgatgacca actccacgct gccgccgccg atgtcgatgc acaacgccgg
     1621 tggcggatcg atcacgacgc tggcccgcac cgccgcgaag atgagccggg cctcttcgag
     1681 gccgctgatg acctcgacct cgacgcccgt ttcggcctcg atgcggtcga cgagctcgct
     1741 gctgttctcc gcggtgcgga tcgcgctcgt cgccttggcc agcacctccg tcgcgccgac
     1801 ggcctcggcc agtgccgtga gccgccgtac gctggcgacg gcgcgttccg cggccgcgaa
     1861 cccgatcgat ccgtagcggc tcacctcgtc gccgaggcgc agcatctcct tctcgcgggt
     1921 gatcgactcg aacgtgccgt cggggtgcac gtcggcgacg agcagatgga acgagttcga
     1981 cccgaggtcg agcgccgcga tgcgcatgct gccgtcagat tagttgcgta ctgttgcggc
     2041 catggatctc accttcgagt cacgcatgct gatcgacggc aacctcgtcg aggccggcgg
     2101 cgccaagacc tacgacaacg tgaatccggc gaccgaagcg gtcatcgggc cggtcgcgga
     2161 cgggtcggtc gccgacatgc aacgcgcgat cgcagccgcc cgccgtgcgt tcgacgagac
     2221 cgactgggcg acgaaccgct ctttgcgcaa ggagtgcctc acgcagctga aggaagcgct
     2281 cgacaagcac aaagaggatt tgcgtcccca gatcgtcgcg gaggtgggca cgccgatcgg
     2341 cctgacgtac gcgatccagc aagacagctg catcgacgac atgcagtggg acatcgacca
     2401 gatcgacaac tacgagtggg agcacgagct cggcgcgcac gagttcttcg ggttgcgttc
     2461 gaaccggctc gtcagtcgcg aagcgatcgg cgtcgtcggg ttgatcacgc cgtggaactt
     2521 cccgttcatg ctcaacctgt cgaagctcgc gccggcgctc gcggccggtt gcaccgcgat
     2581 cctgaagccg gcacccgaca cgccgtactc cgcgacgtgg atcggcaagc tcgtcgcgga
     2641 ggagaccgac attcccgcgg gcgtgttcaa cgtcgtgacg tcggccgacc ccgccgcggt
     2701 cggtgaagca ctgaccggcg acgaacgcgt cgacatgatc tcgttcacag ggtcgaccgg
     2761 cgtcggcaag cgcatcgccg cacgcggcgc cgacacgttg aagcgcgtgt tcctcgagct
     2821 cggcggcaag tcggcgaaca tcatgctcga cgacgccgat ttctccggcc tcgccagcgc
     2881 cggcatggtg tgcatgcact ccgggcaggg ctgcgcgatc acgactcggt tgttgctgcc
     2941 gcggtcgcgc tacgacgagg gcgtccacaa ggtgcgcgag tcgttcgagt cgttcgcgta
     3001 cggcgacccg aacgacatcg ccaacatggc cggcccgctc atcaacgcgc gccagcgtga
     3061 gcgcgtgctc ggctacatcg agaagggcaa ggccgagggt gcaaagtgtc tggttggtgg
     3121 gggaccggcg acgcagttcg agcgcggtta cttcgtgcag ccgacactgt tcgtcgacgt
     3181 cgacccgtcg tcgacgattg cgcaggagga gatcttcggt ccggtgttgt cggtgatccc
     3241 gtacgaagac gacgacgacg cggtccgcat cgcgaacgcg tcgcgctacg ggctgtcggg
     3301 cgcggtcagc agcgcgtcgc tggaccgcgc gctggcggtc gcccgccgca tccgcaccgg
     3361 caccatcgcg gtgaacggcg ggcagtggtt cggccccgac tcgcccttcg gcggctacaa
     3421 ggagagcggc ctgggccgcg agcacggcgt ccagggcttc gaggaatacc tcgagaccaa
     3481 gaccatcggc ctccccgcct aaacgaactg gcgtgagttc ggcgcgagtg gcgcgccgca
     3541 cccacgccgg ttcgtgcgta cctccgaact ggcgtgagtt cggcgcgagt ggcgcgcggc
     3601 acccacgccg gttcggtcgt gtcacaccgg ttcgcgaggc tgtcgggatg ctgcaccggg
     3661 cggatcacga actcttcgaa ctggccgagg accaagacgg catcttcacc aaccatgatg
     3721 ccgacgagcg ggggctcacg cgtccgcagg gaaagcgtcg gatcggaagc ctctgggtgc
     3781 cgatgcaccg cggcgtccac cgaatggccg gtgcgccgct cacgcagcgc gggc
//
LOCUS       DASUGA010000221         2061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1533349, whole genome shotgun sequence.
ACCESSION   DASUGA010000221 DASUGA010000000
VERSION     DASUGA010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533349"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VFX21_04375"
     CDS             complement(<1..467)
                     /locus_tag="VFX21_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX5095220.1"
                     /translation="MNEAFIVDAVRSPVGRRNGGLAKVHPADLGAHSIKALMDKVDVD
                     PAAVEDVVFGCVDTIGPQAGDIARTCWLVAGMPDEVPGTTVDRQCGSSQQAVHFAAQA
                     VMSGTSDLVVAGGVQNMSAIPISAAMLAGQEFGFETPFVGSPGWEARYGNQEVS"
     gene            complement(566..1465)
                     /locus_tag="VFX21_04380"
     CDS             complement(566..1465)
                     /locus_tag="VFX21_04380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5095221.1"
                     /translation="MKFAFSDDQLAFRDAVRDLLEKECSATALRDAWTNETGRVPGLW
                     DQLVEMGVVAMLVSEAQGGLGLSMVDLVLILEETGRYAVPEPIVETAAFGLPLLGRSD
                     VTVSTGAQYLPWADTADVLIALFRQYDSATTELVPHASVDGARRLFELRGDSTPVSGD
                     TGAAYTRAQIGVAAQLCGLSDRMLAMTADYVKERKQFGVAVGSFQAVKHHLANARVAL
                     EFARPLTYRAAATLELVHADMALAKASDAAAVTAKAALQCHGAIGYTTEYDLHLYMKR
                     AWALMRTYGDARYHRGRVAEAIL"
     gene            complement(1462..>2061)
                     /locus_tag="VFX21_04385"
     CDS             complement(1462..>2061)
                     /locus_tag="VFX21_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5095222.1"
                     /translation="HKGLTYFLVDLSTPGVTVRGVERLDGDEGFAEVFFDDVFVADAD
                     VLGTVNEGWGVAMATTGSERGLTLRSPGRFLATAQRLIDLYHARGDDPALRDRLVAAY
                     IDAEAYRWQTFWTVTRIVDGRRTGPESSMVKVFWSELDVELHSLALELLGHEAELLDG
                     PDAAWMKGYQFALAGPIYAGTNEIQRNVIAERVLGLPRK"
BASE COUNT          334 a          720 c          672 g          335 t
ORIGIN      
        1 gacacctctt ggttgccgta gcgcgcttcc cagcccggcg aaccgacgaa cggcgtctcg
       61 aaaccgaatt cctggcctgc gagcatcgcg gcgctgatcg ggatcgcgct catgttctgc
      121 acgcctcccg caacgacgag atcgctcgtg cccgacatca ctgcctgggc tgcgaagtgc
      181 accgcctgct gcgacgaacc gcactgccgg tcgacggtcg tgcccggcac ctcgtcgggc
      241 atgccggcga cgagccagca cgtccgcgcg atgtcgcccg cttgcggacc gatcgtgtcg
      301 acgcagccga acacgacgtc ttcgacggcc gcggggtcga cgtcgacctt gtccatcaac
      361 gccttgatgc tgtgcgcacc caggtcggcc gggtgcacct tcgccaaccc gccgttgcgg
      421 cggccgacgg gggagcgaac tgcgtcaacg atgaaggctt cattcatgcg gcttcgccct
      481 ccggtcctcg atcgccgttg caccttggtt cattgtccgg acctcccggc tgcgcctggc
      541 caggcacgaa gcgaaggata cgagatcaga ggatcgcttc ggccacccgg ccgcggtggt
      601 agcgagcgtc gccataggtg cgcatgagcg cccatgcccg cttcatgtac aggtgcaggt
      661 cgtattcggt tgtgtagccg atcgcgccgt ggcactggag cgccgctttc gccgtgaccg
      721 cggccgcgtc ggatgccttg gcgagcgcca tgtcggcgtg tacgagctcg agcgtcgcgg
      781 ccgcgcgata cgtgagcggt cgcgcgaact cgagcgcgac acgcgcattc gcgaggtggt
      841 gcttcacggc ttggaacgaa cccaccgcga cgccgaactg cttgcgctcc ttcacatagt
      901 cggccgtcat cgccagcatg cgatcgctca agccgcacaa ctgcgcggcg acaccgatct
      961 gcgcccgcgt gtacgcggcg ccggtgtctc cgctgaccgg cgtgctgtcg ccgcgcagct
     1021 cgaacaggcg gcgcgcacca tctaccgacg cgtggggaac aagttcggtc gttgccgagt
     1081 cgtactgccg gaagagcgcg atcaggacgt cggcggtgtc cgcccacggt aagtactggg
     1141 cgccggtgct caccgtgacg tcactgcgcc cgagaagcgg tagcccgaac gccgcggtct
     1201 cgacgatcgg ttccggcacc gcgtagcggc cggtctcttc gagaatcagc accaggtcga
     1261 ccatcgacag gccgagcccg ccttgcgcct cggagacgag catcgcgacg acgcccatct
     1321 cgacgagctg gtcccacaag cccggcaccc ggccggtctc gttcgtccag gcgtcgcgca
     1381 acgcggtcgc gctgcactcc ttctccagga gatcgcggac ggcatcgcgg aacgcgagtt
     1441 gatcgtcgga gaacgcgaac ttcatttgcg cggcaatccc agcactcgtt cggcgatgac
     1501 gttgcgctga atctcgttcg tccccgcgta gatcgggccc gccaacgcga actggtagcc
     1561 cttcatccac gccgcatcgg gtccgtcgag caattccgcc tcatggccga gcagctccaa
     1621 cgccagtgag tgcagctcga cgtcgagctc cgaccagaac accttgacca tcgacgattc
     1681 gggcccggtg cgccggccgt cgacgatccg cgtcacggtc cagaacgttt gccagcggta
     1741 cgcctcggcg tcgatgtacg ccgcgacgag gcggtcgcgc aacgcggggt cgtcgccccg
     1801 cgcgtgatac aggtcgatca agcgttgcgc ggttgcgagg aagcgcccag gggagcgcag
     1861 cgtgaggccg cgctcggaac ccgtcgtcgc catagcgacg ccccagcctt cgttgacggt
     1921 gccgagcacg tcggcatcgg cgacgaacac gtcgtcgaag aacacctcgg cgaacccttc
     1981 gtcgccgtcg aggcgttcga cgccgcgcac ggtcacgccc ggagtcgaaa ggtcgacgag
     2041 gaagtacgtg agacctttgt g
//
LOCUS       DASUGA010000222         2161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2065028, whole genome shotgun sequence.
ACCESSION   DASUGA010000222 DASUGA010000000
VERSION     DASUGA010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2065028"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2161
                     /locus_tag="VFX21_04390"
     CDS             <1..>2161
                     /locus_tag="VFX21_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX5095223.1"
                     /translation="NEYKTASARDPQFETSLHNQCGQKGPALDLALGISQGRDDVLIA
                     VLDSGIKWRDTGDMQDLATKVFINSGEAAPPCTTPDGDADCNHDGVFDITDFGAIADR
                     NANALADPEDLILDPAYANGVDDDHNGYVDDISGWDFVYGDNNPLDSVRYGHGTGEAK
                     DSTAAPDNGGEVGTCPKCRVLPVRVGRSFIAEGGRFAAGVLFALDSGADVVQEALGAI
                     SNPPQAQQAIDAAYDRNVVVVASMADEAAKHPNLPSSLEHTMTVNSVTELDSPFATGI
                     HGYLALNGCTNFGGRTFISIPSGSCSSEATGRSAGMVGLVLSVARDHNLQLTTNEIMQ
                     LVRATADDVDFATPNATEPANDFDPHLAGLVPTMRYPTTPGWDATFGYGRINTYELLK
                     AVRDQRIPPVADITSPQWFDLLDTKGTIKVEGTVGAPRATSYDYRLEWAPGVQPPNHP
                     ATDTWHVVEQRTNQKKTRSGVLGKISLADVAAALPDGGHGVPSDPVTTRPDEERYSVR
                     VRVVVTAHGGSGDGLTGEHQKQVFVHHDDQLARGYPERIPGAGSGSPVFAQLDKTPQP
                     ELVLATDDGRVEVFRNEHKRMKNFPVHTDTPPWWPVASPTAKATHIPPPGSAIGIGAP
                     AIADLDADGTPEIVITDTSGFVWAWNTDGSLQEGAWPAHLDPAFSVDDPATADNLNRV
                     EPGFVAAPAVGDLDGDGTLEVVAAALDRHVYAWHADGS"
BASE COUNT          385 a          781 c          709 g          286 t
ORIGIN      
        1 caacgagtac aagaccgcat cggcacgcga tccgcagttc gagacgtcgc tgcacaacca
       61 gtgcggccag aagggacccg cactcgacct cgcgctcggc atcagccagg gtcgcgacga
      121 cgtgctcatc gccgtcctcg actccggcat caagtggcgc gacaccggcg acatgcagga
      181 tctcgcgacg aaggtgttca tcaacagcgg tgaggcggcg ccgccctgca cgacaccgga
      241 cggcgacgcc gactgcaacc acgacggcgt gttcgacatc accgacttcg gcgccatcgc
      301 cgaccgcaac gcgaacgcgc tcgcggatcc cgaggacttg atcctcgacc ccgcgtatgc
      361 gaacggcgtc gacgacgacc acaacggcta cgtcgacgac atctcgggct gggacttcgt
      421 gtacggcgac aacaacccgc tcgactcggt gcgttacggc cacggcaccg gcgaagcgaa
      481 ggactcgacc gcggcccccg acaacggcgg cgaggtgggt acctgtccga agtgtcgcgt
      541 gcttcccgtg cgcgtcggcc gttcgttcat cgcggaaggt ggtcgcttcg cggccggcgt
      601 cttgttcgcg ctcgacagcg gcgccgacgt cgtgcaggaa gcgctcggcg cgatcagcaa
      661 cccgccacag gcgcagcagg cgatcgacgc cgcgtacgac cgcaacgtcg tggtcgtcgc
      721 gtcgatggcc gacgaagcgg cgaagcaccc gaacctgccg agctcactcg agcacacgat
      781 gacggtgaac tccgtgaccg agctcgactc cccgttcgcg actggaatcc acggctacct
      841 cgcgttgaac gggtgcacga acttcggcgg gcgcacgttc atctcgatcc cgtcgggctc
      901 ctgctcgtcg gaagcgacgg gccgctccgc gggcatggtc gggctcgtgt tgtcggtcgc
      961 gcgcgaccac aacctccaac tcacgacgaa cgagatcatg caactcgtgc gggcgaccgc
     1021 cgacgacgtc gacttcgcca cgccgaacgc gaccgaaccg gccaacgact tcgacccaca
     1081 cctcgcgggc ctcgtgccga cgatgcgcta cccgacgaca ccgggctggg acgcgacgtt
     1141 cggctacggc cgcatcaaca cgtacgagct gctgaaggcg gtgcgcgacc agcgcatccc
     1201 acccgtcgcc gacatcacgt cgccgcagtg gttcgacctg ctcgacacca agggcacgat
     1261 caaggtcgaa ggcaccgtcg gcgcgccgcg cgcgacgtcg tacgactacc gcctcgaatg
     1321 ggcgccgggc gtgcagccgc cgaaccatcc cgcgaccgac acgtggcacg tcgtcgagca
     1381 gcgcaccaac cagaagaaga cacggagtgg cgtgctcggc aagatctcac tcgccgacgt
     1441 cgcggcggcc ttgcccgacg gcggccacgg cgtcccgagc gacccggtca ccacgcggcc
     1501 ggacgaggag cgctacagcg tgcgcgtccg ggtggtcgtg accgcgcacg gtggctccgg
     1561 tgacggactc accggcgagc atcagaagca ggtgttcgtg caccacgacg accagctcgc
     1621 ccgcgggtat ccggaacgaa ttcccggcgc cggctccggc agtccggtgt tcgcgcagct
     1681 cgacaagacg ccgcagccgg agctcgtcct cgcgaccgac gacgggcgcg tcgaagtgtt
     1741 ccgcaacgag cacaagcgga tgaagaactt cccggtgcac accgacacgc cgccgtggtg
     1801 gccggtcgcc tcgccgaccg cgaaggccac gcacatcccg ccgccgggca gtgcaatcgg
     1861 tatcggcgca cccgcgatcg cggacctcga cgcggacggc acgccggaga tcgtgatcac
     1921 cgacacgagc gggttcgtgt gggcgtggaa cacggacggt tcgttgcaag agggcgcgtg
     1981 gcccgcgcat ctcgatcctg cgttcagcgt cgacgatccc gcgactgccg acaacctcaa
     2041 ccgcgtcgag cccggtttcg tcgcggcacc cgcggtcggc gacctcgacg gcgacgggac
     2101 cctcgaagtg gttgcggcag cgctcgaccg tcacgtgtac gcgtggcacg cggacggctc
     2161 t
//
LOCUS       DASUGA010000223         2720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_501230, whole genome shotgun sequence.
ACCESSION   DASUGA010000223 DASUGA010000000
VERSION     DASUGA010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501230"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="VFX21_04395"
     CDS             complement(<1..913)
                     /locus_tag="VFX21_04395"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX5095224.1"
                     /translation="MSVDGYHELPPEQRRVRRLSAELRERESVMPLELFFDLVFVLAF
                     TQCTALMEHESTWSGLGKGILALAVIWWAWVCYAWLTSVVEPEQGAARIVLLLAMAGM
                     LVIALCVPEAFGDRALLFAIAYGLVRAGHIGLFVKVSRDVPEMRRTVLTFACSTALAI
                     ALLVVASFTDGTVQAGLWIASLSVDCLGASFRVDGWSLVPRHFAERHNLVIILALGES
                     IVALGIGAQVDLDAAVITAAVLGVGLAAALWWIYFDVVALVTERRLIRAAPGPERNRL
                     ARDSYSFLHFPMVAGIVLSAFGLHEVLV"
     gene            complement(930..2549)
                     /locus_tag="VFX21_04400"
     CDS             complement(930..2549)
                     /locus_tag="VFX21_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5095225.1"
                     /translation="MTRDHDLSYASGPSDVPLIGRTIGADLLRTVERLPDHEALIDVP
                     TARRWTYAQLNDAIDRVASAFLHQGAAKGDRVGIWSPNCAEWFFVQYATAKIGAILVN
                     INPAYRSHELQYVLNQSSISRLIAAPSFKTSDYVAMVDEVRGECSGLHDVVFFGSERW
                     NEIASCEIDAAALDERGATLTFDDAINIQYTSGTTGFPKGATLSHHNILNNGYFVGRL
                     LDYTERDRVCVPVPFYHCFGMVLGNLAATSTGATIVIPAPAFDPAATLQAVQDERCTS
                     LYGVPTMFIAELGLANFADYDLTSLRTGIMAGAPCPVEVMKRVCAEMNMGEVTIMYGM
                     TETSPVSTMTRRDEDLERRVSTVGTVLPHVEVQIVDPVSGRTVPRGTPGEACTRGYSV
                     MIGYWDAPEQTAEAIDGAGWMHTGDLATMDDDGFIAIVGRSKDMVIRGGENIYPREIE
                     EFLYGHPVIDEVQVIGVPDEKYGEELMAWVRCKPETTLTIDELRAFCDGKLAHYKIPR
                     YLHLTDEFPMTVTGKIRKVEMRERAVDILRP"
BASE COUNT          471 a          907 c          883 g          459 t
ORIGIN      
        1 gcaccagcac ctcgtgcaac ccgaacgcgc tgagcacgat gccggcaacc atcgggaagt
       61 gcagaaacga gtacgagtcg cgcgcgagcc ggttgcgttc gggtccgggc gcggcgcgga
      121 tgaggcggcg ctcggtgacc agcgcgacga cgtcgaagta gatccaccag agcgcggctg
      181 ccaatcccac gccgagcacc gccgcggtga tcacggcggc atcgagatca acttgtgcgc
      241 cgatcccgag cgcgacgatc gactcgccga gcgcgaggat gatgacgagg ttgtggcgct
      301 cggcaaagtg ccgtggaacg aggctccacc cgtcgacccg gaagctcgcg cccaggcagt
      361 cgaccgagag cgaggcgatc cagagcccgg cctgcaccgt gccgtcggtg aacgacgcga
      421 cgacgaggag ggcaatggcg agtgcggtgc tgcacgcgaa cgtgagcact gtgcgccgca
      481 tctccggtac gtcgcgactt actttgacga agagcccgat gtggccggcg cgcacgagac
      541 cgtacgcgat cgcaaacagc agcgcgcggt caccgaatgc ttccggaaca cagagggcga
      601 tgacgagcat gcccgccatc gcgagcagca acacgatgcg cgccgcacct tgttcgggct
      661 cgacgacgct cgtgagccat gcgtagcaga cccatgccca ccagatcaca gccagcgcga
      721 ggatgccctt gcccagaccg gaccacgtcg actcgtgctc catcagcgcg gtgcactgcg
      781 tgaacgcgag cacgaacacg agatcgaaga acagctcgag cggcatcaca ctctcccgct
      841 cgcgcagctc cgcgctcaat cgccgcacgc gccgctgttc gggcggcaac tcgtggtatc
      901 cgtcgacgct catgctccca gcatgcgcgc tatgggcgaa ggatgtcgac cgcccgctcg
      961 cgcatttcga ccttgcggat cttgcccgtc acagtcatcg ggaactcgtc ggtgaggtgc
     1021 agatatcgcg ggatcttgta gtgcgccagc ttcccgtcac agaacgcgcg cagttcgtcg
     1081 atcgtgagtg tcgtctcggg tttgcagcga acccaggcca tgagctcttc gccgtacttc
     1141 tcgtcgggca caccgatcac ctgcacttcg tcgatcacag gatgcccgta caagaactct
     1201 tcgatctcgc gcggatagat gttctcgccg ccccggatga ccatgtcctt gctgcgcccg
     1261 acgatggcga tgaatccgtc gtcgtccatc gtcgcaaggt cgccggtgtg catccatccc
     1321 gccccgtcga tggcttccgc cgtctgctcg ggcgcatccc agtatccgat catcaccgag
     1381 tagccacgtg tgcacgcctc gcccggcgtt cctcgcggga ccgttcgccc cgacaccggg
     1441 tcgacgatct gcacctccac atgcgggagc acggtcccga cggtcgagac ccgacgctcg
     1501 agatcttcgt cgcgtcgtgt catcgtggag acgggtgacg tctcggtcat gccgtacatg
     1561 atcgtgacct cgcccatgtt catctcggcg cacacccgct tcatcacctc gaccgggcac
     1621 ggcgcgccgg ccatgatgcc cgtacgcaac gacgtcaggt cgtagtcggc aaagttcgcg
     1681 agaccgagtt cggcaatgaa catcgtcggc acgccgtaca gcgaggtgca ccgttcgtcc
     1741 tgcacggctt gcagcgtcgc ggcggggtcg aacgccggcg ccggaatgac gatcgtcgcg
     1801 ccggtcgatg tcgcggcaag gttgccgagc accatgccga agcagtggta gaagggcacc
     1861 ggcacgcaga cgcgatcgcg ctcggtgtag tcgaggagcc ggcccacgaa gtagccgttg
     1921 ttcaagatgt tgtggtgcga caacgtcgcg cccttcggga agccggttgt tcccgacgtg
     1981 tactggatgt tgatcgcgtc gtcgaacgtc aaggtggcgc cgcgttcgtc gagcgccgcg
     2041 gcgtcgatct cgcacgacgc gatctcgttc catcgctcgc tgccgaagaa gaccacgtcg
     2101 tgcaggccgc tgcactcgcc gcgcacctca tcgaccatgg ccacgtagtc gctcgtcttg
     2161 aacgacggcg ccgcgatcaa gcgcgagatc gacgactggt tcagcacgta ctggagctcg
     2221 tgactccggt acgcggggtt gatgttcacg aggatcgcgc cgatcttcgc ggtcgcgtac
     2281 tgcacgaaga accactccgc gcagttcgga gaccagatgc cgacgcggtc acccttggca
     2341 gctccctgat gcaggaacgc tgaggcgacg cgatcgatcg cgtcgttcag ctgggcgtag
     2401 gtccaccggc gcgcggtcgg cacgtcgatc agcgcttcgt ggtcgggcag tcgctcgacg
     2461 gttcgcagca gatcggcgcc gatcgtgcgg ccgatgagcg gcacgtcgct cggaccactc
     2521 gcgtagctga ggtcatggtc tcgagtcatc gccgaccccc tcgtcgccgt ctgtctactc
     2581 cgacgggcgc cgatggtcga gcgataggtt tcgccgatgg ccttcgaaac ggtgaccggg
     2641 tactgctggc cgcaatcggt cgccggtggt gacccggtcg cgttgcacct gtcgtcgccg
     2701 ggtgggaggg cggtcgcggt
//
LOCUS       DASUGA010000224         2356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_465215, whole genome shotgun sequence.
ACCESSION   DASUGA010000224 DASUGA010000000
VERSION     DASUGA010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465215"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VFX21_04405"
     CDS             <1..418
                     /locus_tag="VFX21_04405"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020520776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5095226.1"
                     /translation="AVFSSDLTRARETAEIAFGDGPIPIFFDWRLRECDYGAANGSPA
                     DQHVRRRADHLEQPYSGGESWQEAVARVTRCIDDIAARWDGGRVIVIGHVATRWALDH
                     MVHGTPLTDLVAQDFAWREGWEYYVVRGSGPSGSIS"
     gene            complement(376..>2356)
                     /gene="aceE"
                     /locus_tag="VFX21_04410"
     CDS             complement(376..>2356)
                     /gene="aceE"
                     /locus_tag="VFX21_04410"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX5095227.1"
                     /translation="DEPESTASLAIAAREELDNLIFVVNCNLQRLDGPVRGNHKVIQE
                     YESIFRGMGWNVIKVIWGSAWDDLLARDNDGVLVNKMNATLDGEFQKYAVESGAYIRD
                     HFFGPDPRLRSMVEHLSDEDLQKLPRGGHDYRKLYAAYKAATEHEDAPTVILAKTIKG
                     WTLGELFEARNATHQIKKMTSEHLKAFRDRLYLDIPDADLEEGDLPPYYHPGYRSPEY
                     EYLMERRRALDGYLPERVDRAKTVIFPKDDVIDQLAAGTGEKVQASTTTAFTRFLRTL
                     LKDPEFGRRIVPIIPDEARTFGMDALFPEYKIYAPFGQQYEPVDAGLMLSYREGAKGQ
                     ILEEGITEAGSMADFTAAATAYATWGQPMVPFFIFYSMFGFQRVGDLIWQFGDMRGRG
                     FLMGATAGRTTLTGEGLQHCDGQSQMYALAYPNCRAYDPAFAYEVAVLVREGIRRMYG
                     PEPEDCFYYLSLYNENYSQPAMPEGVEEGIVRGMYLYRAAEGEHEHRAQILASGPMVL
                     QALDAQRMLADDHNVGADVWSVPGWKQLRDDAIDCERWNRLHPNDAPRTPYVTEQLDA
                     TQGPIVGVSDWVKTVPDSISRFVPQPYVVLGTDGSGWSDIRPSLRRHFEVDAAHIVLG
                     VLDGLAQTGDVKGEELAEAIARYEIDPDGPDPRTT"
BASE COUNT          343 a          780 c          815 g          418 t
ORIGIN      
        1 cgccgtcttc agctccgatc tgacccgtgc ccgggagacc gccgagatcg cgttcggcga
       61 cggcccgatc ccgatcttct tcgactggcg gctgcgcgag tgcgactacg gcgcggccaa
      121 tggcagcccg gccgaccagc acgtccggcg ccgcgccgac catctcgagc agccctattc
      181 gggcggcgag agctggcagg aggccgtcgc gcgcgtcact cggtgcatcg acgacatcgc
      241 ggcgcgctgg gacggcgggc gcgtgatcgt gatcggccac gtggcgacgc gctgggcgct
      301 cgaccacatg gtgcacggca cgccgctcac cgacctcgtc gcgcaggact tcgcgtggcg
      361 agaagggtgg gagtactacg tagtccgcgg gtcggggccg tcggggtcga tctcgtagcg
      421 cgcgatcgct tcggccagct cctcgccctt gacgtcaccc gtctgcgcga ggccgtcgag
      481 gacacccagc acgatgtgcg cggcgtcgac ctcgaagtgg cggcgcagcg agggccggat
      541 gtcggaccag ccggagccgt cggtgcccag caccacgtag ggctgcggca cgaagcgtga
      601 gatcgagtcg gggacggtct tcacccagtc gctgacgccg acgatcgggc cttgcgtcgc
      661 gtcgagctgc tcggtgacgt acggcgtacg cggcgcgtcg ttcgggtgca gacggttcca
      721 gcgttcgcag tcgatcgcgt cgtcgcgcag ctgcttccag ccggggacgc tccacacgtc
      781 ggcgccgacg ttgtggtcgt cggcgagcat ccgttgcgca tcgagcgcct gcaacaccat
      841 cgggccgctc gcgaggatct gcgcgcggtg ctcgtgctca ccttcggccg cgcggtacag
      901 gtacatgccg cgtacgatgc cctcttcgac gccctccggc atcgccggct gcgagtagtt
      961 ctcgttgtag agggacaggt aatagaagca gtcttcgggc tccggcccgt acatgcgtcg
     1021 gatgccctcg cgcacgagca ccgcaacctc gtacgcgaac gcggggtcgt acgcgcgaca
     1081 gttcggatac gccaacgcgt acatctgcga ctgcccgtcg cagtgctgca gcccttcacc
     1141 ggtcaatgtc gtgcggccgg cagtcgcgcc catgaggaaa ccgcggccac gcatgtcgcc
     1201 gaactgccag atgaggtcgc cgacgcgctg gaagccgaac atcgaataga agatgaagaa
     1261 cgggaccatc ggctggcccc acgtcgcgta cgcagtggcg gccgcggtga agtcggccat
     1321 gctgcccgct tcggtgatcc cctcttcgag gatctggccc ttcgcgccct cgcggtacga
     1381 aagcatcaga cccgcgtcga ccggctcgta ctgctggccg aacggcgcat agatcttgta
     1441 ctcggggaac agcgcgtcca tgccgaacgt gcgcgcttcg tcggggatga tcggcacgat
     1501 gcgcctgccg aactccgggt ccttcaacag cgtgcgcagg aaccgggtga acgcggtcgt
     1561 cgtcgatgct tggaccttct cgccggtgcc cgccgcgagc tggtcgatga cgtcgtcctt
     1621 cgggaagatc accgtcttcg cccggtcgac gcgctcgggc aggtagccgt cgagcgcgcg
     1681 tcggcgttcc atcaggtact cgtactccgg tgagcggtag ccggggtggt agtacggcgg
     1741 caggtcgccc tcttcgagat ccgcgtcggg gatgtcgagg tagagccggt cgcggaacgc
     1801 cttgaggtgc tccgacgtca tcttcttgat ctggtgcgtc gcgttgcggg cctcgaacag
     1861 ctcgcccagc gtccagccct tgatcgtctt cgcgaggatc acggtgggcg cgtcttcgtg
     1921 ctccgttgcg gccttgtagg cggcgtagag cttgcggtag tcgtgcccac cgcgcggcag
     1981 cttctgcagg tcttcgtcgc tgaggtgctc gaccatcgaa cgcaggcgcg ggtcggggcc
     2041 gaagaagtgg tcgcggatgt atgcgccgct ctcgaccgcg tacttctgga actcgccgtc
     2101 gagggtcgcg ttcatcttgt tgacgagcac gccgtcgttg tcgcgggcga gcaagtcgtc
     2161 ccaggcgctg ccccagatca ccttgatgac gttccagccc atgccgcgga agatcgattc
     2221 gtattcctgg atgaccttgt ggttgccgcg caccgggccg tcgagtcgct gcaggttgca
     2281 gttcacgacg aagatcaggt tgtcgagctc ctcccgcgcc gcgatggcga ggctcgcggt
     2341 ggactcgggc tcgtcc
//
LOCUS       DASUGA010000225         4633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1351065, whole genome shotgun sequence.
ACCESSION   DASUGA010000225 DASUGA010000000
VERSION     DASUGA010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351065"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2540)
                     /locus_tag="VFX21_04415"
     CDS             complement(<1..2540)
                     /locus_tag="VFX21_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018535888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5095228.1"
                     /translation="MAPDGADNGTLDRFSAPVRAWFETSFAAPTDAQAAGWPAIAARD
                     HTLILAPTGSGKTLAAFLWAIDRLATAPEPAKDRRCRVLYVSPLRALAVDVEKNLRAP
                     IAGIGLAAERLGVPSRTPTVGMRTGDTPAKERQALVRTPPDILITTPESLYLMLTSRA
                     RETLRSVDCVIVDEIHALAPTKRGAHLMLSLERLDELCDTPPQRIGLSATQRPLDEIA
                     RFLGGHSAEGPRPVTIVDAGSRKPLELEVIVPVDDMSELHANPPPQPIDDANFDLTPE
                     PRASIWPHVHPRLLELIRAHHTTLIFCNARRMSERLAAQLNDLAGEELVRAHHGSLSR
                     EQRLVIESDLKAGRLRAIVATSSLELGIDMGSVDLVVLVESPGSVARGMQRVGRAGHQ
                     VGEPSTGKIFPKYRGDLLEATAVTRRMRDGLVEEMRFPRNPIDVLAQQIVAATAVDEW
                     DVDALLALIRRSANFAELSDDVYREVLDLLAGRYPSDRFAGLRPRVVWDRVQNRVRAR
                     EGAQRVAVTSGGTIPDRGLFGVFLPDGARVGELDEEMVYESRAGETFVLGASTWRIEE
                     ISFDRVVVTPAPGEPGKTPFWKGEKPGRPLELGRALGAVVRELRALPAERAEAVLRDD
                     GLDERAAANLRTYLEEQAESTGAVPDDRTIVIERFPDEIGDWRVCILTPFGSRVHAPW
                     ALALEERLTRHDMPVQVLWSDDGIILRLPEALDDIPLDLLLFDPDEVEELVVERLPNT
                     SLFASRFRENAARALLLPRRRPGERTPLWQQRQRAADLLEVASGYPTFPMLLETTREC
                     LRDVFDLPALREVLTDIRSRKVSVVPVETKRASPFAQSLLFGWIAVYM"
     gene            2650..2985
                     /locus_tag="VFX21_04420"
     CDS             2650..2985
                     /locus_tag="VFX21_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5095229.1"
                     /translation="MKLACDRSAPAHARHALVEAFACLATPIRQQSELIVSELVTNAV
                     LHGSEPIVFRADRRGDRLRIEVHDGSTRMGPPTEHSRGLELIARVASAWGVQRHAPGK
                     TVWAEMLLD"
     gene            complement(3023..>4633)
                     /locus_tag="VFX21_04425"
     CDS             complement(3023..>4633)
                     /locus_tag="VFX21_04425"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX5095230.1"
                     /translation="VVHEIAHMWFGDLVTMKWWNGIWLNEAFATFMEHAGVDAFRPEW
                     RTWDDLALSRAAAMDIDALSHTRAVEYEVHTPEDAEGMFDVLTYQKGGSVVRMLEQWL
                     GADAFRAGVRHYLDRYRYANTETTDLWDSLEAATSQPARRIMDSWIFQPGFPEVVVEP
                     SLAGVRLSQRRFTYADDTDATTWSIPVLARVDDEVHQLLVEGDGISLDVPGDARVVVN
                     AGGEGFYRVAYPPVMRATLLTHGRLNARERFAIVDDAWAAVLANTATVAEYVDLLDSF
                     VNEPDLVVWRTILGRARDATRLVDGPALDALRARLAAILRPTFDRLGWDPASGEDGRE
                     RQLRGLVLDSLATVGRDDEIAAEAKVRHERVGTDPDVVAACIAIIADRGGEAEFDDYV
                     RRFETSDTPQEQLRYLYALMQFPTRELVLRAAQLASSDAVRSQNGPFLLQRGLKHRVH
                     GPAVWEFVHHNWGAIDDRFPRTLMSRMLEGITWLVDDQTARDVPAFLAEHPIKEGAKV
                     IAQHLERQRIHRTLWEREHDRFATELLS"
BASE COUNT          684 a         1589 c         1668 g          692 t
ORIGIN      
        1 tacatgtaca ccgcgatcca gccgaacagg agcgactgcg cgaacggtga cgcccgtttc
       61 gtctcgaccg ggaccacgct caccttgcgc gagcggatgt cggtgagcac ttcgcgcagc
      121 gcggggaggt cgaacacgtc gcgcaggcac tcgcgcgtcg tctccagcag catcgggaac
      181 gtcgggtacc cggacgcgac ctccaacagg tcggccgcgc gctggcgctg ttgccacaac
      241 ggcgtgcgct cacccggccg gcggcgcggc aacaacaacg cgcgtgccgc gttctcgcgg
      301 aaccgggacg cgaacaacga agtgttcggc agccgctcga ccacgagctc ctcgacctcg
      361 tcggggtcga acagcaagag gtcgagcggg atgtcgtcga gcgcttcggg gagccgcagg
      421 atgatgccgt cgtcggacca cagcacttgc accggcatgt cgtggcgggt gagccgctct
      481 tcgagcgcga gcgcccaggg tgcatggacc cgcgagccga acggtgtgag gatgcacacg
      541 cgccagtcgc cgatctcgtc ggggaagcgc tcgatcacga tcgtgcggtc gtcggggacc
      601 gcgcccgtgg actcggcctg ctcctccaag tacgtgcgta ggttcgcggc cgcacgctcg
      661 tcgaggccgt cgtcgcgcaa cacggcttcg gcccgctcgg ccggcagcgc gcgaagctcc
      721 cgcacgaccg cgccgagggc gcggcccagc tccaacgggc ggcccggctt ctcgcccttc
      781 cagaacggtg tcttgcccgg ttcgccgggc gcgggggtga cgacgacccg gtcgaaggag
      841 atctcctcga tgcgccatgt cgacgcgccg agcacgaacg tctcgccggc gcggctctcg
      901 tagaccatct cttcgtcgag ctccccgacg cgggcaccgt cggggaggaa gacgccgaac
      961 agaccgcggt cggggatcgt gccgccactc gtgaccgcga cgcgctgtgc accttctcgc
     1021 gcccgcactc ggttctgcac gcggtcccac acgacgcgcg ggcgcaagcc ggcgaagcga
     1081 tcggacgggt agcgccccgc gagcaggtcg aggacttccc ggtacacgtc gtcggagagc
     1141 tccgcgaagt tcgccgagcg gcggatcagc gcgagcagcg cgtcgacgtc ccactcgtcg
     1201 accgcggttg cggcaacgat ctgttgcgcg agcacgtcga tcgggttgcg tgggaaccgc
     1261 atctcttcga cgaggccgtc gcgcatgcgg cgcgtgacgg ccgtggcttc gaggaggtcg
     1321 ccgcggtact tcgggaagat cttgcccgtg ctcggctcgc ccacctggtg gccggcccgc
     1381 ccgacgcgct gcatgccgcg cgccaccgag cccggcgact cgaccaacac cacgaggtcg
     1441 acgctgccca tgtcgatgcc gagctcgagg ctgctcgtcg cgacgatcgc gcgcagccgc
     1501 cccgccttga gatcggattc gatgacgagc cgttgctcgc gcgagagcga cccgtggtgg
     1561 gcgcgtacga gctcctcgcc ggcaaggtcg ttcaactgcg ccgcgagccg ctccgacatg
     1621 cggcgcgcgt tgcagaagat cagcgtcgtg tggtgcgcgc ggatcaactc caacaagcgc
     1681 ggatgcacgt gcggccagat gctcgcccgc ggctcgggcg tgagatcgaa gttcgcgtcg
     1741 tcgatcggtt gcggcggcgg gttcgcgtgc agctccgaca tgtcgtcgac cggcacgatg
     1801 acttcgagct cgagcggctt gcgcgagccg gcgtcgacga tggtgaccgg acgcggaccc
     1861 tcggccgagt gcccgccgag gaagcgggcg atctcgtcga gcggtcgctg cgtcgccgag
     1921 agaccgatgc gctgcggcgg tgtgtcgcac agttcgtcga gccgttccaa gctcaacatg
     1981 aggtgcgcac ctcgcttcgt cggcgcgagc gcgtggatct cgtcgacgat cacgcagtcg
     2041 accgagcgca gtgtttcgcg cgcccgcgac gtcaacatca gatagagcga ctcgggcgtg
     2101 gtgatgagga tgtcgggagg ggtgcggacg agcgcctgcc gctccttggc cggcgtgtca
     2161 ccggtgcgca tcccgacggt cggcgtgcgc gacggcacgc cgagccgctc ggccgcgagg
     2221 ccgatgcccg cgatcggtgc ccgcaggttc ttctcgacgt cgacggccaa cgcgcgcaac
     2281 ggcgacacgt acagcacgcg gcagcggcgg tctttcgccg gctcgggcgc ggttgcgagc
     2341 cggtcgatcg cccacaagaa cgcggcgagc gtcttgcccg agcctgtcgg cgccaggatc
     2401 agcgtgtggt cgcgcgccgc gatcgcgggc caccccgcgg cctgggcgtc ggtgggcgcg
     2461 gcgaagctgg tctcgaacca ggcccggacc ggcgccgaga accggtcgag cgtcccgttg
     2521 tccgcgccgt cgggagccac ggattccaag gtaccggtgc cctgcgacaa cgaatccggg
     2581 tcgggtttca ctcggttgga gccgggaacg ctcggctccg tgcatgggcc gacacgggac
     2641 gcgctcgaaa tgaagctcgc atgcgatcgg tccgcgcccg cccacgcccg gcatgccctg
     2701 gtcgaggcgt ttgcgtgcct ggcgacgccg atccgccagc agtcggagtt gatcgtgagc
     2761 gaattggtga cgaacgcggt gctccacggc tccgaaccga tcgtcttccg ggccgaccgg
     2821 cgcggtgacc gcctccggat cgaggtgcac gacgggtcga cccgcatggg accaccgacc
     2881 gagcattccc gcggcctgga gctcatcgcc cgagtcgcgt cggcctgggg cgtgcaacgg
     2941 cacgcgcccg gaaagaccgt ctgggccgag atgctccttg actgaccggc gcggaactgc
     3001 gccctgcgct cgctctacac cgctaggaaa gaagttcggt cgcgaaccgg tcgtgctctc
     3061 gttcccagag cgtacggtgg atccgctggc gctcgaggtg ttgcgcgatg accttcgcgc
     3121 cttccttgat cgggtgctcg gcgaggaacg cgggcacgtc gcgcgcggtc tggtcgtcga
     3181 cgagccaggt gatgccctcg agcatgcgcg acatcagtgt gcgggggaac cggtcgtcga
     3241 tcgcgcccca gttgtggtgc acgaactccc acaccgccgg gccgtgcacg cggtgcttca
     3301 gcccgcgctg gaggaggaac gggccgttct gtgaccgcac ggcgtcggag ctcgcgagct
     3361 gcgcggcccg cagcaccaac tcccgcgtcg ggaactgcat cagcgcgtag aggtagcgga
     3421 gctgctcttg gggtgtgtcc gacgtctcga agcgacgcac gtagtcgtcg aactcggcct
     3481 cgccgcctcg gtcggcgatg atcgcgatgc acgcagccac gacgtcgggg tcggtgccga
     3541 cgcgttcgtg gcggaccttc gcctccgcgg cgatctcgtc gtcgcgtccg acggtcgcga
     3601 gcgagtcgag gacgagcccg cgcagctggc gctcgcgccc gtcttcgccg ctcgcggggt
     3661 cccagccgag gcggtcgaac gtcggccgga ggatcgcggc aaggcgggcg cgcagggcgt
     3721 cgagcgcggg gccgtcgacg aggcgggtcg cgtcgcgggc gcggccgagg atcgtgcgcc
     3781 acacgacgag gtcgggttcg ttcacgaagc tgtcgagcag gtcgacgtat tcggcgacgg
     3841 tcgcggtgtt cgcgaggacc gccgcccacg cgtcgtcgac gatcgcgaat cgctcgcgcg
     3901 cgttcaggcg gccgtgggtg agcagggtgg cgcgcatcac cggcggatac gcgacccggt
     3961 agaagccttc accgcccgcg ttgacgacga cacgcgcgtc gccgggaacg tcgagcgaga
     4021 tgccgtcgcc ctcgacgagc aactggtgca cctcgtcgtc gacgcgcgcg agcaccggga
     4081 tcgaccaggt tgtcgcgtcg gtgtcatcgg cgtaggtgaa acgccgctgc gagagccgca
     4141 cgcccgccaa cgacggttcg accacgacct cggggaagcc gggctggaag atccacgagt
     4201 ccatgatccg gcgggccggc tgcgacgttg ccgcctcgag cgaatcccag aggtcggtgg
     4261 tctcggtgtt cgcgtagcgg taccggtcga ggtagtggcg cacgccggcg cggaacgcgt
     4321 ccgcgcccaa ccactgttcg agcatgcgga cgactgaacc gcccttctgg tacgtgagga
     4381 cgtcgaacat cccctccgcg tcctcgggcg tgtgcacttc gtattcgacc gcgcgcgtgt
     4441 gcgagagcgc gtcgatgtcc attgcggccg cgcgtgagag cgcgaggtcg tcccaggtgc
     4501 gccactcggg ccggaacgcg tcgacgcccg cgtgctccat gaacgtcgcg aacgcttcgt
     4561 tcagccagat gccgttccac cacttcatcg tgacgaggtc gccgaaccac atgtgcgcga
     4621 tctcgtggac gac
//
LOCUS       DASUGA010000226         2529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_118070, whole genome shotgun sequence.
ACCESSION   DASUGA010000226 DASUGA010000000
VERSION     DASUGA010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118070"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VFX21_04430"
     CDS             <1..286
                     /locus_tag="VFX21_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095231.1"
                     /translation="TQTAQVLELIEHLRDTGLGIVVISHNIDVVFEVADRIVVLYVGR
                     VMATFERASTTRDEVVSAIVGVALGLGAPRALLTPWTLHRDCKGRDVSRS"
     gene            complement(371..1537)
                     /locus_tag="VFX21_04435"
     CDS             complement(371..1537)
                     /locus_tag="VFX21_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5095232.1"
                     /translation="MARAGIPSPRRPPLRAFPGMLRGRTSPPRRRAQSDRLRIALLCP
                     VWFPVPPPGYGGIEAVVALLAEVLTGAGHDVTVFASGDSAPMARLSSIYPTAPSERIG
                     ESIVELRHVLACYERADTFDVISDHTGPVAAALSRFVECPVLHTVHGPLDGELGDAYE
                     QVARVAPGLGLVSLSLSQRSPRPLLPWVANCPNAIDPSRYSCSLRRGDYLVFVGRMGH
                     EKGCRHAIAAAKSVGLPLKIAAKCREPAERAYFAQYVEPQLGHDVEYVGEVGHDEKVE
                     LLRNARATLFPVDWEEPFGLVMIESLACGTPVVATRRGSVPEVLEEGRTGVIVDDHEA
                     LPAAIADADRIDRAECRRAVEERFAPRSLLAAYTRAYRTAINRPTCVQQRVFQP"
     gene            complement(1537..1857)
                     /locus_tag="VFX21_04440"
     CDS             complement(1537..1857)
                     /locus_tag="VFX21_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095233.1"
                     /translation="MSAQTGHDESCWTDEYLIAHSEGYRVDDERRQLVGVVDSVVWPD
                     EPFEDAEAVVVRCAGRPEHIVIPVEEIREVDPWDKRIVVSARSVLPVSPASPPPLSST
                     HPVG"
     gene            2074..>2529
                     /locus_tag="VFX21_04445"
     CDS             2074..>2529
                     /locus_tag="VFX21_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095234.1"
                     /translation="MLDVTAPTPRRRAPRDDLPQGLRRRFEAIVFDWDGTAVTDRAAD
                     ATRLRAIIEELSTQGMHLAVVTGTHVSNVDRQLGARPTGPGRVLLSVNRGSELFEADG
                     DGLRLLFRREATASEEAALDAAAAATVEELGRRGIDATIVSQRLNRRKID"
BASE COUNT          428 a          835 c          865 g          401 t
ORIGIN      
        1 cacgcagacc gctcaggtgc tcgagctgat cgagcatctg cgggacaccg ggctcggcat
       61 cgtcgtcatc tcccacaaca tcgacgtcgt gttcgaagtc gccgatcgca tcgtcgtgct
      121 gtacgtggga cgcgtcatgg ccacgttcga gcgagcttcg accacgcgcg acgaagtggt
      181 aagcgcgatc gtcggcgtcg ctcttggtct cggcgcacct cgagcgctgc taaccccttg
      241 gacgctccat cgggattgca aagggcgtga cgtatcacga tcttgagctc gcatttggtt
      301 cggtgggtgg caagggcgcg ttgtccgcga cgtctgagat gcaggcggac gcagtcgctc
      361 tcgggcgacg tcagggctga aagacgcgct gttgtacgca ggttggtcgg ttgatcgccg
      421 tccgataagc gcgcgtgtag gccgcaagca gagaacgggg tgcgaatcgc tcctccacgg
      481 cccggcgaca ctctgcgcgg tcgatgcggt cggcgtcagc gattgccgct ggcagcgctt
      541 cgtggtcgtc gacgatgacg cccgtgcgtc cctcctccag cacctcgggg accgagccgc
      601 gtcgggtagc gacgacgggc gtgccgcacg ccagagactc gatcatcacg aggccgaacg
      661 gttcctccca gtcgacgggg aagagcgtcg ctcgtgcgtt ccgcaggagc tcgactttct
      721 cgtcgtggcc gacctctccg acgtactcga cgtcgtggcc gagctgtggc tcgacatact
      781 gcgcgaagta ggcgcgctct gcgggctcac ggcattttgc ggcgatcttg agcggcaggc
      841 cgaccgactt cgcggcggcg atcgcgtggc ggcagccctt ctcgtggccc atcctgccga
      901 cgaagaccaa atagtcgcca cgtcgcaggc tgcacgagta gcgcgacggg tcgatcgcgt
      961 tcgggcagtt ggcgacccac ggaaggagcg ggcgggggct ccgctgactc agtgacaacg
     1021 acacgagtcc caatccgggc gcgacgcgcg caacttgttc gtacgcgtcc cctagctcgc
     1081 cgtcgagcgg gccgtgcacc gtgtgcagca ccgggcactc gacgaaccgt gacagggctg
     1141 ctgcgaccgg accggtgtga tcgctgatga cgtcgaacgt gtcggcgcgc tcgtagcacg
     1201 ccagcacgtg tcgaagctcc acgatcgact ctccgatgcg ctcgctcggt gccgtcgggt
     1261 agatggagga gagtctggcc atcggggcgg agtcccccga ggcaaagaca gtgacgtcgt
     1321 ggccggcacc ggtcagcacc tcggcgagca gtgcaaccac tgcttcgata cccccgtaac
     1381 cgggaggcgg caccggaaac cagaccgggc agagcaacgc aatccgcagg cgatccgact
     1441 gcgcgcgccg ccgcggcgga gaagtccgcc ccctcaacat gcctgggaaa gccctcagcg
     1501 gcggccgcct tggggagggg atgcctgctc gcgccatcac cccacgggat gtgtggacga
     1561 cagcggagga ggagacgccg gcgacaccgg aaggacgctt ctggcggata cgacgattcg
     1621 cttgtcccac gggtcgactt cgcggatctc ttcgacggga atcacaatgt gctcgggtcg
     1681 gcccgcgcaa cgcaccacga cggcctcggc gtcctcgaag ggctcgtcgg gccacaccac
     1741 gctgtcgaca acgccgacga gttgccgacg ctcgtcatcg acgcggtacc cctcggagtg
     1801 ggcgatcagg tactcgtccg tccagcagga ctcgtcgtgg cctgtctgag cgctcatctt
     1861 cccaacctcc tgttaccaaa agcatcgccg aacgatcgac ggccgccatc agggggccgg
     1921 ctgagagagg tgcgtagaag cccgcacatc gatcagcgca ggggcgttga tgtgtcgtgg
     1981 tggcccccac ttgcgaggat gacgtgatga gccattcgtg cgtgtcctcc ctgacgccct
     2041 ctcgcgccgt gtgatggggc ttcccgcgcg tccatgctgg acgtgaccgc accgacgccc
     2101 agacgacgtg caccgcgcga cgacctgcca cagggcctcc gccgacggtt cgaagcgatc
     2161 gtgttcgact gggacgggac ggcggtcacg gaccgggccg ccgacgcgac gcgcctgcgc
     2221 gcgatcatcg aggagctgtc cacccaaggt atgcatctgg ccgtcgtaac cggcacgcac
     2281 gtcagcaacg tcgaccggca gctcggcgca cgcccgacag ggccgggacg tgtcctcctc
     2341 agcgtcaacc gagggtcaga actgttcgaa gccgatggag acgggttgag gctgctgttt
     2401 cggcgagagg cgacggcctc ggaggaggcg gccctcgacg cggccgccgc ggcaaccgtc
     2461 gaggagctgg ggcgccgcgg gatcgacgcg accatcgtgt cgcaaaggct caaccgccga
     2521 aagatcgac
//
LOCUS       DASUGA010000227         2657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1159278, whole genome shotgun sequence.
ACCESSION   DASUGA010000227 DASUGA010000000
VERSION     DASUGA010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159278"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1958
                     /gene="lon"
                     /locus_tag="VFX21_04450"
     CDS             <1..1958
                     /gene="lon"
                     /locus_tag="VFX21_04450"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX5095235.1"
                     /translation="RELAREYHAIVENILETRGARRMLDLLPGVDDPGGLADTAAYSP
                     ELTMEQRIQLLETLDVEARLRLAITWGREQLADLNLKDKIRTEVSDDLEQQQREMLLR
                     RQMDAIRKELGDGDDDVVGEYRAKLADKKFPDEVRAAVEKEIDRLERMGQQSPEHSWV
                     RTWLDATFDLPWGERAEENNDLDAARATLDADHEGLDKVKERILEFLAVRTLRAERGL
                     AAEGGRGSGAILVLVGPPGVGKTSLGESVARALGRPFVRVAVGGVRDEAEIRGHRRTY
                     VGSRPGRIVRALTEAGAMNPVLLIDEIDKLQAGGWSGDPASALLEVLDPAQNHTFRDH
                     YLEVDLDLSDVLFLATANQLETIPGPLLDRMELVQLDGYTEDEKVAIAKRHLFPRQRE
                     KAGLRDDELSVTDGALHAIVSGWTREAGVRGLERQLGTIARKATRKIATGQAATVVVD
                     EPDVKDMLGRQRFKPEDATRAPIPGLATGLAVTGAGGDVLTIEVTGMDGDPGLQVTGQ
                     LGEVMSESAHIALSFIRANADELGVPATAFEKRKFHLHVPAGAVPKDGPSAGITMTTA
                     LTSLLTGRPVKPGVGMTGEVTLQGRVLPIGGLKQKLLAAHRAGLTDVVIPADNEPDLD
                     DVPEQVREQLHVHPVHDVREVLAVALS"
     gene            1979..>2657
                     /locus_tag="VFX21_04455"
     CDS             1979..>2657
                     /locus_tag="VFX21_04455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5095236.1"
                     /translation="MGALEGRVAIVTGGGAGIGRAVVERFVAEGARVVFAEIDADRAR
                     DAQVAAGEQSVAVVCDVREPASAHALVTAACDAFGRVDILVNNVGHYGGARKAFHEQS
                     DAEWDELYRVNLGHVLWCSREVIPVFLEQGDPAAIVNVSTIEAFRAIPTRAVYSAYKS
                     ALTGLTRSLAVEYGGRGIRVNAIAPDVTETAQVPYSRWVGPDEQHMIPSWVPVGRFGQ
                     PADTAAVA"
BASE COUNT          422 a          897 c          969 g          369 t
ORIGIN      
        1 cacgcgagct cgcacgtgag taccacgcga tcgtcgagaa catcctcgag acgcgcggcg
       61 cgcgccggat gctcgacctg ttgcccggcg tcgacgaccc gggcggtctc gccgacacgg
      121 ccgcgtactc gcccgagctc acgatggaac aacgcatcca gctgctcgag acgctcgacg
      181 tcgaagcgcg gctgcggctt gcgatcacgt ggggtcgcga gcagctcgcc gatctcaact
      241 tgaaggacaa gatccgcacc gaggtctccg acgacctcga gcagcaacag cgcgagatgc
      301 tgctgcgccg ccagatggac gccatccgca aggagctggg cgacggtgac gacgacgtgg
      361 tgggcgagta ccgcgccaag ctcgcggaca agaagttccc cgacgaagtg cgggccgcgg
      421 tcgaaaagga gatcgaccgg ctggagcgca tggggcaaca gagccccgag cactcgtggg
      481 tgcgcacgtg gctcgacgcg accttcgacc taccgtgggg tgagcgcgcg gaggagaaca
      541 acgacctcga cgccgcgcgc gcgacgctcg acgcggacca cgaagggctc gacaaggtca
      601 aggagcgcat cctcgagttc ctcgcggtgc gcacgttgcg agccgagcgc ggtctcgcgg
      661 ccgagggcgg ccgcggttcg ggcgcgatcc tcgtgctcgt cggcccgccg ggtgtcggca
      721 agacgtcgct cggtgagtcg gtggcgcgcg ccctcggtcg cccgttcgtg cgggtcgccg
      781 tcggcggtgt gcgcgacgaa gcggagatcc gcggtcaccg gcgtacgtat gtcgggtcgc
      841 ggcccgggcg catcgtgcgc gcgctgaccg aggcgggcgc gatgaacccg gtgctgttga
      901 tcgacgagat cgacaagctg caggccggcg gctggagcgg cgaccccgcg tccgcgctgc
      961 tggaggtgct cgaccccgcg cagaaccaca cgttccgcga ccactacctc gaagtcgacc
     1021 tcgacctttc cgacgtgttg ttcctcgcga ccgcgaacca gttggagacc atccccggcc
     1081 cgctgctcga ccgcatggag ctggtgcagc tcgacggcta caccgaggac gaaaaggtcg
     1141 cgatcgccaa gcgccacctt ttcccgcgcc agcgcgagaa ggcggggctg cgcgacgacg
     1201 agctgtcggt caccgacggc gcgttgcacg cgatcgtcag tggttggacg cgtgaagcgg
     1261 gcgtgcgtgg tctcgagcgt cagctcggca ccatcgcgcg caaggcgacg cgcaagatcg
     1321 cgacgggcca ggcggcgacg gtcgtcgtcg acgagcccga cgtgaaggac atgctcggcc
     1381 ggcagcggtt caagcccgag gacgcgacac gcgcaccgat ccccggattg gcgacgggcc
     1441 tggcagtgac cggcgccggt ggtgacgtac tcaccatcga agtcaccggc atggacggcg
     1501 acccgggcct gcaggtcacg ggccagctgg gcgaggtcat gagcgagtcc gcgcacattg
     1561 cgctgtcgtt catccgcgcc aacgccgacg agctcggcgt gccggcgacc gcgttcgaga
     1621 agcgcaagtt ccacttgcac gtgcccgcgg gcgcggtgcc caaggacggg ccgtcggccg
     1681 gcatcacgat gacgaccgcg ctgacgagcc tgctcacggg gcggccggtg aagcccggtg
     1741 tcggcatgac cggcgaggtc acgttgcagg gccgggtgtt gccgatcggc ggtttgaagc
     1801 agaagctgct cgcggcgcac cgcgccggtc tgaccgacgt ggttatcccc gccgacaacg
     1861 agcccgacct cgacgacgtg cccgagcagg tgcgcgagca actgcacgtg cacccggtgc
     1921 acgacgtgcg tgaggtcctc gccgtcgcgc tgagctgatc cgtgtacctt cgagcgcgat
     1981 gggcgcgctc gaaggacgcg tagcaatcgt caccggcggc ggcgcaggca tcggccgcgc
     2041 cgtcgtcgag cgcttcgtcg ccgaaggtgc gcgcgtcgtg ttcgccgaga tcgacgcgga
     2101 ccgggcgcgc gacgcccaag tcgcggcggg ggagcagtcg gttgccgtcg tgtgcgacgt
     2161 gcgcgaaccg gcgtccgcgc acgcgctggt caccgccgcg tgcgacgcgt tcggtcgcgt
     2221 cgacatactc gtgaacaacg tcggccacta cgggggcgcg cgcaaggcgt tccacgagca
     2281 gagtgatgcc gagtgggacg agctgtaccg cgtgaacttg gggcacgtgc tgtggtgctc
     2341 gcgcgaggtc atcccggtgt tcctggagca aggtgatccg gctgcgatcg tcaacgtgtc
     2401 gacgatcgaa gcgtttcgcg cgatcccgac acgcgcggtc tactcggcgt acaagtcggc
     2461 gctgaccggc ctcacgcgca gtctcgcggt cgagtacggc gggcgcggga tccgcgtcaa
     2521 cgcgatcgcg cccgacgtca ccgagaccgc acaagtgccg tactcgcgct gggtcgggcc
     2581 cgacgagcag cacatgatcc cgtcgtgggt gcccgtcggg cgcttcgggc agcccgccga
     2641 taccgccgcg gtcgcgc
//
LOCUS       DASUGA010000228         6364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1585305, whole genome shotgun sequence.
ACCESSION   DASUGA010000228 DASUGA010000000
VERSION     DASUGA010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585305"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VFX21_04460"
     CDS             complement(<1..528)
                     /locus_tag="VFX21_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095237.1"
                     /translation="MPTELEPLGDVAWAAAVDYVQKLASHEYESATDCLESAIATEGI
                     GAMCNALGDACRSLMDRVTFEAGTIDVHTVVEQIAQRVVAVAGDTRPDALDHQRSLIV
                     FLGSEGLPCAARADVATWSPIERLRALVACTIGLLGIAGENEGTEIVDLVGTLDKPGD
                     PAPAPGTFGLAWRGNE"
     gene            731..2053
                     /locus_tag="VFX21_04465"
     CDS             731..2053
                     /locus_tag="VFX21_04465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5095238.1"
                     /translation="MLSALAVAALALRVLYIVRTQGDVVSGDGTYYHALAAFLADGRG
                     FIAPELYEATGRMMPNASHPPVWPVVLAGPAALGLRTFFEQQLFTALIGSAVVVAVGV
                     AGRLVAGAGAGLIAAALAAIYPNLWLYEREVLSEPLAMLLAACTIILAYRFIERPGIG
                     RAGALGALCAVLAQTRSEQMLLFPFLVMPLVLRTHTVPLPRRLLWLGASAAIALVLLA
                     PWSAYNANRFERPVFLSTQFGAGFLTSNCDETYHGERLGSVHPQCSAQNRRDVDLSGD
                     PTEDDAQMRDEGLRYVRAHLSRVPVVVLAREGRVWNVYRPSQQMEIDSSRDTNKSVIR
                     LGYVAYWALLPFAVAGVLLLHRRRVALLPLLVFPAITAIAVAVQFGYTRYRAPAEVSI
                     LILSAVTLDAIVRRVRRPPPPSRVVTLPAEEPSEILSVHGFDPQDHLR"
     gene            2022..3236
                     /locus_tag="VFX21_04470"
     CDS             2022..3236
                     /locus_tag="VFX21_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014054282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5095239.1"
                     /translation="MVSIPKIISVDDHVIEPPNVWQDRLPEKFKAIGPRIERRKIKSM
                     KFVGGVFSYEEAGANEDGTYCDWWLVEDLASPLTRLSAAAGYPRDEVTVSPITMDDMR
                     PGCWSQKERLEDMDVNHVEASLAFPSFPRFCGQTFTERKDKELGDLCVKAYNDWMIDE
                     WCAGSNGRLIPLIIVQLWDAKLAAAEIRRNAARGCRAVTFSEIPPFLGLPSIHDKDGY
                     WDPFLAACEETGTVVNMHIGSSSKMPSTSADAPPAVGSTLTYMNAAMSLTDWLMSGVF
                     ERFPDLKIAYSEGQIGWIPYVLERADKVWEDNRGWGGVADKVKRPPSEYYFEHIYGCF
                     FDDPHGLANIDAVGRDNVTFETDYPHSDSTWPHTKKVAEEIMKDLDQETVNKVCRDNA
                     IKMLSLDFPTAN"
     gene            3237..3818
                     /locus_tag="VFX21_04475"
     CDS             3237..3818
                     /locus_tag="VFX21_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017840425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX5095240.1"
                     /translation="MPRVCVYAGSNFGARPAYRAAAESLARAIVGRGMGIVYGGSDIG
                     LMGAVADAGLAAGGEVIGVMPQALVDHEIAHRGLTELRITASMHERKATMADLADGFV
                     ALPGGVGTLEELVEIVTWAQLGLHAKPCGLLDVAGYYAQLQAFLDHAAAEAFIRPQHR
                     ALLLVEDDPDALLDAFESWRAPTVEKWIDRASS"
     gene            complement(3808..4692)
                     /locus_tag="VFX21_04480"
     CDS             complement(3808..4692)
                     /locus_tag="VFX21_04480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011434535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX5095241.1"
                     /translation="MDLGPVGIWTFCLDGQPSARAREIAAELESLGYGSLWIPEVMGR
                     DPFVAASGLLAGTERLVVGTGIASVWARDALAMKSAWYTVEEAHPDRFILGLGVSHQP
                     FVDGMRAATYEKPLSKMRAYLDAMDMAPFAASPPTTTPKRVLAALGPKMLALAGEKTD
                     GAHPYNGTPEHTAAAREIMGPGKLLAVEQKVVCVEDAAAAREIARGMLRVYTPLPNYA
                     NNWKRLGFTDDDLADGGNDRFVDTVVAWGSEDTIKRRVQAHLDAGADHVCIQVLTAEG
                     FAARPIDEWRRLAPALTS"
     gene            4742..5224
                     /locus_tag="VFX21_04485"
     CDS             4742..5224
                     /locus_tag="VFX21_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095242.1"
                     /translation="MKLAALAAVAVLAFAGCGGDDDDSSGGGGGSDDTVSEAEFQKQA
                     NALCVKFNDEVDAKSEELNSSLDENSTPEDIANVFIDEILPLFRDQVQELQDLPTPDA
                     NAEQYDELFDDLESAADDLEQQAKDDPEKLFSSEEDPFADLDQRARDLGLDDCGSTDE
                     "
     gene            5265..>6364
                     /locus_tag="VFX21_04490"
     CDS             5265..>6364
                     /locus_tag="VFX21_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5095243.1"
                     /translation="MTRQLLIGGEWVDGADGGYDVVNPATETVVDVAPEASVAQAQAA
                     AAAARAAFDSWSQTTPEYRADLLAKAADAIKARYQELLPLVIAETGCTETVGKQMQVP
                     TAWTRFEAYARYALESNTIPLAPQEMPATALAPGGLMGAVVRRAPVGVVACITPYNFP
                     IVNMAGKLGPALAMGNTVVVRPASQNPLAVIELVRILHEVGFPEGVVNVVTGSTPASG
                     EALVDSRDVDMVSFTGSTGVGTRIAEGCGRTMKRTLLELGGKGAALVFDDADVKTAVS
                     MIGSVWTFHSGQICTSPTRAIIQRKLYDQVVAGLSKMAGVLKVGDPYEPGVVVGPVIT
                     AAHRDRVEGYVQIGRDEGGEVVAGGARPEHPD"
BASE COUNT          969 a         2174 c         2200 g         1021 t
ORIGIN      
        1 ctcgttgccc cgccacgcga gcccgaaggt gcccggcgcc ggtgccgggt caccgggctt
       61 gtcgagcgtg ccgaccaggt cgacgatctc ggtcccttcg ttctcgcccg cgatgccgag
      121 cagcccgatc gtgcacgcca cgagcgcgcg caggcgctcg atcggtgacc acgtcgcgac
      181 gtcggcgcgt gccgcgcacg gcagaccttc actgcccagg aacacgatga gggatcgttg
      241 atggtccagt gcgtcgggac gagtgtcgcc cgcgacggcg acgacgcgct gcgcgatctg
      301 ctcgacgacg gtgtgcacgt cgatcgtgcc cgcctcgaac gtcacgcggt ccatcaacga
      361 ccggcacgcg tcgcccaacg cgttgcacat cgcgccgatg ccttcggtcg cgatcgcgct
      421 ctcgaggcag tcggtcgcgg actcgtactc gtggctcgcc agcttctgca cgtagtcgac
      481 ggcggcggcc cacgcaacgt caccgagtgg ttcgagttcg gtgggcatcg gccctcctcg
      541 gctcgcgact gctccgcttc tttatcggcg aacggcaccg aaatctgagg tggccccctc
      601 ccgagttgtc cgttttgctc ggacaaggcc gtcggaactt cgtgcggcgg cttgggaggc
      661 gaccggcatt gcgggattgt cactcgcgat gacgggacgc gggccgagcg gggctcgacg
      721 cttctgggca gtgctcagcg ccctcgcggt cgccgcgctc gcactgcgcg tgctctacat
      781 cgtccggacg cagggcgacg tcgtcagcgg cgacggcacc tactaccacg cgctcgcggc
      841 cttcctcgca gacggccgcg gcttcatcgc accggagttg tacgaggcga cgggccggat
      901 gatgccgaac gcgagtcacc caccggtgtg gcccgtcgtc ctcgcgggtc ccgcggcact
      961 cggccttcgc acgttcttcg agcagcagct cttcaccgcg ctgatcggct cggccgtcgt
     1021 cgtcgcggtg ggcgtcgcgg gtcgcctcgt ggcgggcgcc ggcgcgggcc tgatcgcggc
     1081 ggcgctcgcg gccatctatc ccaacttgtg gctgtacgag cgcgaagttc tctccgagcc
     1141 cttggcgatg ctgctcgccg cgtgcacgat catcctcgcg taccggttca tcgaacggcc
     1201 gggcatcggg cgtgccggcg cgttgggcgc gctgtgcgcg gtgctcgcgc agacccgttc
     1261 ggaacagatg ctcctgttcc cgttcctcgt catgccgctc gtcctgcgca cgcacacggt
     1321 gccactcccg cggcgcctcc tctggctcgg tgcgtccgcc gcgatcgccc tggtgctcct
     1381 cgcgccctgg tccgcgtaca acgcgaaccg cttcgagcga ccggtgttcc tctcgacgca
     1441 attcggcgcc ggcttcctga cgtcgaattg tgacgagacc tatcacggtg agcgtctcgg
     1501 ctcggtccac ccgcagtgct cggcgcagaa ccgtcgcgac gtggatctca gcggagaccc
     1561 aaccgaagat gacgcgcaga tgcgcgacga aggactgcgc tacgtccgcg cgcatctttc
     1621 ccgcgtcccg gtcgtcgtcc ttgcgcgcga aggtcgtgtg tggaacgtct accgcccgtc
     1681 ccaacagatg gagatcgaca gctcgcgcga cacgaacaag agcgtcatcc ggctcggcta
     1741 tgtggcctac tgggcgctgc tgcccttcgc cgttgcgggc gtcctcctgc tgcaccgccg
     1801 gcgcgttgcg ttgctgccgc tgctcgtgtt cccggcgatc accgcgatcg ccgtcgcggt
     1861 gcagttcggg tacacgcggt accgggctcc cgccgaggtg agcatcctga tcctttcggc
     1921 cgtcacgctc gatgcgatcg tgcggcgggt ccggcgccca ccaccgccga gcagggtggt
     1981 gacgctcccg gccgaggagc cgtcggagat actgtccgtg catggtttcg atccccaaga
     2041 tcatctccgt tgacgaccac gtcatcgagc cgccgaacgt ctggcaggac cgcctgccgg
     2101 agaagttcaa ggcgatcggg ccccgcatcg agcggcgcaa gatcaagtcg atgaagttcg
     2161 tgggcggtgt cttctcgtac gaggaggcgg gtgccaacga ggacggcacc tactgcgact
     2221 ggtggctggt cgaggatctc gcgtcgccgc tgacccgcct ctcggccgcg gccggctacc
     2281 cgcgcgacga agtgaccgtg agcccgatca cgatggacga catgcggccc ggctgctggt
     2341 cgcagaagga acggctcgag gacatggacg tcaaccatgt cgaggcgtcg ctcgcctttc
     2401 cgagcttccc acgtttttgc gggcagacgt tcaccgagcg caaggacaag gagctcggtg
     2461 acctctgcgt caaggcgtac aacgactgga tgatcgacga gtggtgcgcg ggttcgaacg
     2521 gccgcttgat cccgctcatc atcgtgcagc tctgggacgc caagctcgcg gccgccgaga
     2581 tccggcgcaa cgcggcgcgc ggttgccgcg cggtgacgtt ctcggagatc ccgccgttcc
     2641 tcgggctgcc gtcgatccac gacaaggacg gctactggga tccgttcctc gccgcgtgcg
     2701 aagagacggg cactgtcgtc aacatgcaca tcggttcgtc gtcgaagatg ccctcgacgt
     2761 cggcggacgc gccgcccgca gtcggttcga cgctcacgta catgaacgcg gcgatgtcgt
     2821 tgaccgactg gttgatgtcg ggcgtgttcg agcggttccc ggacttgaag atcgcgtaca
     2881 gcgaaggcca gatcggttgg atcccctacg tgctggagcg cgccgacaag gtgtgggagg
     2941 acaaccgcgg gtggggtggc gttgccgaca aggtgaagcg cccgccgtcg gagtactact
     3001 tcgagcacat ctacggctgc ttcttcgacg acccgcacgg gttggcgaac atcgacgcgg
     3061 tcggccgcga caacgtgacg ttcgagaccg actatccgca cagcgactcg acgtggccgc
     3121 acaccaagaa ggtcgcggaa gagatcatga aggatctcga ccaggagacc gtcaacaagg
     3181 tgtgccgcga caacgcgatc aagatgctgt cgctcgactt cccgacggcc aactgattgc
     3241 cgcgcgtctg cgtctacgcg gggtcgaact tcggcgcgcg ccctgcatat cgcgccgcgg
     3301 ccgagtcgct ggcacgggcg atcgtcggcc gcggcatggg cattgtgtac ggcggcagcg
     3361 acatcgggtt gatgggtgcg gtcgccgatg ccggactcgc ggcgggcggc gaggtcatcg
     3421 gcgtcatgcc gcaggcgctc gtcgaccacg agatcgcgca ccgcgggctg acggagctgc
     3481 gcatcaccgc gtcgatgcac gagcgcaagg cgacgatggc cgatctcgcc gacggattcg
     3541 tcgcgctgcc cggtggcgtg ggcacgctcg aagagctcgt cgagatcgtc acgtgggcgc
     3601 agctcggcct gcacgccaag ccgtgcggcc tgctcgacgt cgccggctac tacgcgcagt
     3661 tgcaggcgtt cctcgaccac gcggcggccg aagccttcat ccgcccccag caccgggcct
     3721 tgctcctggt cgaggacgac cccgacgcgt tgctggacgc cttcgagtcg tggcgcgccc
     3781 ccacggtcga aaagtggatc gaccgtgcta gctcgtgagc gcgggggcga ggcggcgcca
     3841 ttcgtcgatc ggccgcgcgg cgaagccctc ggccgtgagc acctggatgc acacgtggtc
     3901 ggcacccgcg tcgaggtgtg cctgcacgcg ccgcttgatc gtgtcctcgg agccccacgc
     3961 gacgaccgtg tcgacgaagc ggtcgttgcc gccgtccgcg aggtcgtcgt cggtgaaccc
     4021 gaggcgcttc cagttgttcg cgtagttggg gagcggtgtg tagacgcgca gcatcccgcg
     4081 cgcgatctcg cgcgcggcgg ccgcgtcttc gacgcagacg accttctgct cgaccgcgag
     4141 aagcttgccg gggcccatga tctcgcgcgc ggccgcggtg tgctcgggcg tgccgttgta
     4201 cgggtgcgcg ccgtcggtct tctcgccggc gagcgcgagc atcttcggcc cgagcgcggc
     4261 caggacgcgt ttgggtgtcg tggtcggggg gctggcggcg aacggcgcca tgtccatcgc
     4321 gtcgaggtag gcgcgcatct tcgacaacgg tttctcatac gtcgcggcgc gcatgccgtc
     4381 gacgaacggt tggtggctga cgcccagacc gaggatgaac cggtccggat gcgcctcctc
     4441 gaccgtgtac cacgcggact tcattgcgag cgcgtcgcgc gcccacaccg acgcgatgcc
     4501 ggtgccgacc acgagccgct ccgtcccggc gaggagcccg gacgcggcga cgaacggatc
     4561 gcggcccatc acttcgggga tccacagcga gccgtagccg agcgactcca gctccgccgc
     4621 gatctcgcgc gcgcgtgccg aaggctgccc gtcgaggcag aacgtccaga tgccaaccgg
     4681 tccgagatcc atacgcggac gctacccagc ggttagcgtc ccccggatgc gacgaggtgg
     4741 attgaagttg gcggctctgg ccgcagtggc ggtcctggca ttcgcgggct gtggcggcga
     4801 cgacgacgac agcagtggcg gcggtggcgg cagcgacgac acggtctcgg aggccgagtt
     4861 ccagaagcag gcgaacgccc tctgcgtgaa attcaacgac gaggtcgacg cgaagtcgga
     4921 ggagctcaac agctccctcg acgagaactc gacgccggaa gacatcgcca acgtgttcat
     4981 cgacgagatc cttccgttgt tccgcgatca ggtacaggag ctgcaggacc tgccgacgcc
     5041 ggacgcgaac gccgagcagt acgacgagct cttcgacgac ctggagagcg cggcggacga
     5101 tctggagcag caggccaagg acgatccgga gaagttgttc agcagcgaag aggatccgtt
     5161 cgcggacctc gaccagcggg cccgggacct cggcctcgac gactgcggct cgaccgacga
     5221 gtgacctgaa cgcacgaaaa tagaacgcgt tacacttcgc gttcatgaca cgtcagctgc
     5281 tcatcggcgg ggagtgggtc gacggggccg acggcggtta cgacgtcgtc aaccccgcga
     5341 ccgagaccgt cgtcgacgtc gcgcccgaag cgtcggtcgc ccaggcgcag gccgccgcgg
     5401 ccgcggcccg cgccgcgttc gactcgtggt cgcagaccac gcccgagtac cgcgccgacc
     5461 tgctggccaa ggcggccgac gcgatcaagg cgcgctacca ggagttgctc ccgctcgtca
     5521 tcgcggagac ggggtgcacc gagaccgtcg gcaaacagat gcaggtgccg accgcgtgga
     5581 cccgcttcga ggcctacgcg cgctacgcgc tggagtcgaa cacgattccg ctcgcacccc
     5641 aggagatgcc ggcaaccgcg ctcgcgccgg gcggcctcat gggtgcggtg gtacggcgcg
     5701 cgccggtcgg cgtcgtcgcg tgcatcacgc cgtacaactt cccgatcgtg aacatggccg
     5761 gcaagctcgg ccctgcgctc gcgatgggca acaccgttgt cgtgcgcccc gcgtcgcaga
     5821 accccctcgc ggtcatcgag ctcgtgcgca tcctgcacga ggtcgggttc cccgaaggcg
     5881 tcgtcaacgt cgtcaccggc tcgaccccgg cgagcggtga agcgctcgtc gacagccgcg
     5941 acgtcgacat ggtgtcgttc accggatcga cgggtgtcgg cacgcgcatc gccgagggct
     6001 gcggccggac gatgaagcgc acgctgctcg agctgggcgg caagggcgcc gcgctcgtgt
     6061 tcgacgacgc ggacgtgaag accgcggtgt cgatgatcgg ctcggtgtgg acgttccact
     6121 cgggccagat ctgcacgtcg ccgacccggg cgatcatcca gcgcaagctc tacgaccagg
     6181 tggtcgcagg cctgtcgaag atggccggcg tcctgaaggt cggcgacccg tacgagcccg
     6241 gcgtcgtcgt cggaccggtc atcaccgccg cgcaccgcga ccgcgtcgag ggctacgtgc
     6301 agatcgggcg cgacgagggc ggcgaagtcg ttgccggtgg cgcgcgaccg gagcaccccg
     6361 acaa
//
LOCUS       DASUGA010000229         2581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_307653, whole genome shotgun sequence.
ACCESSION   DASUGA010000229 DASUGA010000000
VERSION     DASUGA010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307653"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..1943
                     /locus_tag="VFX21_04495"
     CDS             282..1943
                     /locus_tag="VFX21_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEX5095244.1"
                     /translation="MQLIPLRLIAAVVALGAVFPSSSMPDEGMWPFDGLPLQQLKDKY
                     NFEPTKEWLDHVRLGSVRFDTGGSGSFVSANGLVMTNHHVALSTIQKVSSQEKDWVKD
                     GFSSRLYGSELKSVGLKLRQLIDVKDVTKEILAAKAADKTAEGNEWRAKVQEMCDAIT
                     DEEKHVQADPVQLYSGQSFRIYVYHTYDDVRVVFAPEKQVAFFGGDPDNFTYPRYDLD
                     CAFFRVYEDGKPVDSSKFYFKWNAKGAKADELVFVSGHPGNTERLLTHAELEFMRDLR
                     VPQQVAGLKRMYDQCKELMARSEENAFRLRDQFFGISNSLKAYEGHLAGLKDAVMMAK
                     MKTREEELIQKSGKPEVQAAFDSIAKGYAEYRKVIAANESRQAITDFRKKLDDEIVAP
                     AKAVINKARFDVYGNSMYPDATFTLRLAYGTVKGYEAGTTMVPPKTTFNGLYERNAAF
                     DNQAPFDLPKRWIDKKSKLKLDTPINFVCTADIIGGNSGSPVLNRNAEVVGLVFDGNI
                     ESLPGNYWFDERVNRCVSVHTGGMIEAIEVIYEEKDLVRELREGK"
     gene            1975..>2581
                     /locus_tag="VFX21_04500"
     CDS             1975..>2581
                     /locus_tag="VFX21_04500"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX5095245.1"
                     /translation="MTPRQRRPGREGHSLDGIFRPRSVAVIGASRREGAIGRQVVANI
                     VKAGFTGPVYPVNHHADTVLSILAYASIADIPGPVDLAVLVVPADAAVRAAEQCGRKG
                     VKGLVVVTAGFNEIGGKGTARERRLLAIARKYGMRVIGPNCMGIINSEPAYRLDASFA
                     AIYPGEGRVAMVSQSGALGESILSDAASHGLGVAMFASVGNR"
BASE COUNT          467 a          854 c          869 g          391 t
ORIGIN      
        1 gaccgttcgt gaggaggtgt cgctccgtcg ttcagccgct gcgggcgaga gcggagtgaa
       61 gatagtctgc ccgacgggac tgcaactcat gagatccgcg ccatctgccg catcggacga
      121 ccgaggctcc cgcgccgcac cgttcggtga cggacccccg gcccggggca gatcgtcgga
      181 cgcagagtcg cgtactcgga cgtgccccgg ggccacccga tccctatcat gccccgcccg
      241 cgttcccccg ttcggtggag ccgctcccag gaaaccccac gatgcaactc atcccgctcc
      301 ggctgatcgc agccgtcgtc gcgctcggcg ccgtcttccc gtcctcctcg atgcccgacg
      361 agggcatgtg gccgttcgac ggcctgccgc tgcagcagtt gaaggacaag tacaacttcg
      421 aaccgacgaa ggagtggctc gaccacgtgc ggctgggctc ggtccgtttc gacaccggcg
      481 gctccggctc gttcgtgtcg gcgaacgggc tcgtgatgac gaaccaccac gtcgcgctgt
      541 cgacgatcca gaaggtgtcc tcgcaggaga aggactgggt caaggacggc ttcagctcgc
      601 ggctctacgg cagcgagctc aagagcgtgg gcctgaagct gcgccaactg atcgacgtca
      661 aggacgtcac caaggagatc ctcgccgcga aggccgcgga caagaccgcg gaaggcaacg
      721 aatggcgtgc gaaggtccag gagatgtgcg atgcgatcac cgacgaggag aagcatgtcc
      781 aggccgatcc cgtccagctc tacagcggcc agtcgttccg gatctacgtc taccacacgt
      841 acgacgacgt gcgtgtcgtg ttcgccccgg agaagcaggt cgcgttcttc ggcggtgacc
      901 cggacaactt cacctacccg cgctacgacc tcgactgcgc gttcttccgc gtgtacgagg
      961 acggcaagcc ggtcgactcg tcgaagttct acttcaagtg gaacgccaag ggcgcgaagg
     1021 ccgacgagct ggtgttcgtg tccggccacc ctggcaacac cgagcggctg ctgacacacg
     1081 cggagctcga gttcatgcgc gacctccgcg tgccgcagca ggtcgccggg ctgaagcgga
     1141 tgtacgacca gtgcaaggaa ctgatggcgc gcagcgagga gaacgcgttc cggctccgcg
     1201 accagttctt cggcatctcg aacagtctga aggcctacga gggccatctc gccggactga
     1261 aggatgccgt catgatggcg aagatgaaga cgcgcgagga ggagctgatc cagaagtcgg
     1321 gcaagccgga ggtccaggcg gcgttcgact cgatcgcgaa gggttacgcc gagtaccgga
     1381 aggtgatcgc ggcgaacgaa tcgcgacagg cgatcaccga cttccgcaag aaactcgacg
     1441 acgagatcgt ggcgccggcg aaggccgtga tcaacaaggc gcggttcgac gtctacggca
     1501 actcgatgta ccccgacgcg accttcacgc tgcggctcgc ctacggcacg gtgaaggggt
     1561 acgaggccgg cacgacgatg gtgccgccga agacgacgtt caacggcctg tacgagcgca
     1621 acgccgcgtt cgacaaccag gcgccgttcg acctgcccaa gcgctggatc gacaagaagt
     1681 cgaagctgaa gctcgacacg ccgatcaact tcgtctgcac cgccgacatc atcggcggca
     1741 actcgggcag cccggtgctc aaccgcaacg ccgaggtcgt cgggctggtc ttcgacggga
     1801 acatcgagtc gctgccgggc aactactggt tcgacgagcg cgtcaaccgc tgcgtcagcg
     1861 tgcacaccgg cggcatgatc gaggcgatcg aggtcatcta cgaggagaag gacctcgtac
     1921 gcgagctccg cgagggcaag tgaccggacg atctggcgca gccgcgaccc tcgcatgact
     1981 cctcggcagc gtcgtcccgg gcgcgagggt cacagcctcg acggcatctt ccgcccccgt
     2041 tcggtcgcgg tgatcggcgc gagccggcgc gagggcgcga tcggccggca ggtcgtcgcc
     2101 aacatcgtca aggccgggtt caccgggccg gtctatccgg tgaaccacca cgcggacacg
     2161 gtgctgtcga tcctcgcgta cgcgtcgatc gcggacatcc cgggcccggt cgacctcgcg
     2221 gtgctcgtcg ttcccgccga cgccgccgtg cgcgccgcgg agcagtgcgg caggaagggc
     2281 gtgaagggtc tggtcgtcgt caccgcgggg ttcaacgaga tcggcggcaa gggcaccgcg
     2341 cgcgaacgcc ggctcctggc gatcgcccgc aagtacggga tgcgcgtcat cggccccaac
     2401 tgcatgggga tcatcaactc ggagccggcg taccggctcg acgcgtcgtt cgccgccatc
     2461 tatcccggcg aaggccgcgt cgcgatggtc tcccagtcgg gggcgctcgg tgagtcgatc
     2521 ctctcggatg ccgcgagcca cggactcggt gtggcgatgt tcgcgtcggt cggcaaccgc
     2581 g
//
LOCUS       DASUGA010000230         5894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_63805, whole genome shotgun sequence.
ACCESSION   DASUGA010000230 DASUGA010000000
VERSION     DASUGA010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63805"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VFX21_04505"
     CDS             complement(<1..305)
                     /locus_tag="VFX21_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095246.1"
                     /translation="MRHGNPPPDGAALTAIIDRLTRLGARREEIDDAIVSNRLDSLTV
                     EYLLKPDRSLTIAELAARAGASLEDALRLWRAWGFPTPMPTDRRFDASDVGHLRFAL"
     gene            complement(302..1186)
                     /locus_tag="VFX21_04510"
     CDS             complement(302..1186)
                     /locus_tag="VFX21_04510"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX5095247.1"
                     /translation="MASLNTPVIIEAALNGVTSKKRNPHAPIAPDEQAKDGLACVDAG
                     ATVIHTHAANIAVPPEDAALAYAEAYRPIVDAHPGVICYPTTGLGATIDERYRHIELL
                     DDIGVIRAGFVDTGSVNLGGTGPDGLPPASDYVYKNTFSDVAHKMRVCAERGLGPSIA
                     CFEPGFARVVVAYHRAGKLPAGTLVKFYFSGGGYLGGGEPLWGAPPTREALDLYVSML
                     ENTGIPWAVAVLGGSVCDTPVAQLALERGGHLRVGLEDWDTGPSNEEQVARAASLCAE
                     ANRTVASIDEAVQVLGLP"
     gene            1251..2183
                     /locus_tag="VFX21_04515"
     CDS             1251..2183
                     /locus_tag="VFX21_04515"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013754569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX5095248.1"
                     /translation="MRMQNRVTEMLGVEYPIVQAPMGWIARAQLASAVSNAGGLGIIE
                     TSSGMLAEVRGEIAKMRDLTDKPFGVNIAQMFVRDPSIVDFVVDNGVKFVTTSAGDPT
                     KYTKLLKDAGLTVFHVVPSLRGALKAVDAGVDGLVVEGNEGGGFKAPTGASTMVLLPL
                     VCSKVDVPVIAAGGVCDGRSMAAAFVLGAEGVQMGTRMVSAAESPIHDNWKQLICAAG
                     EGDTYLLNRNNPPAFRVLKSAFSVRAEGAGDPVIPNLDNVLDLYFNGNLDASFAFGGQ
                     VAGRIDGVEPVADILESTMREFDATMREAASRYA"
     gene            2203..3141
                     /locus_tag="VFX21_04520"
     CDS             2203..3141
                     /locus_tag="VFX21_04520"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5095249.1"
                     /translation="MGNSGFAVRFKLFRVPVTVHPAFFVILFLFGYLGAAEYILVWVA
                     IGFASILIHEFGHALTARAFGSPARVELVSSGGVTYHNQMSDGRSLLVTLAGPATGIA
                     IGLPLWLLVDETDYAGAVHAALVYGVWISLAWSLLNLLPILPLDGGNAVQSILQLTTG
                     KPHVVTTRRISIVIAAAVAAFAASQGYIFGAFYAVTFIGINLSALRQHQERANAANPA
                     DLNAAQPVDRLQQTGAALLAKGEIDRGLAFITEGIERSGTIDKSVVDVVIATGSTEAL
                     ATKLRHLPHPRGGSWEPVFRAALQQLGYDDKPGTFN"
     gene            complement(3138..3740)
                     /locus_tag="VFX21_04525"
     CDS             complement(3138..3740)
                     /locus_tag="VFX21_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095250.1"
                     /translation="MDGIAGVQSRINEIVGRFSAGSVAGGVLGAGAASDADDFASTLA
                     RAEESQATSASSATSTDTYVAPTGGDLNKAGVDPVQWAKDFLTALGMPTSSENVRAVT
                     AWEQAEGTRAHFNPLATTQGGFAGTSNLNSVGVKNYASYQDGIAANVKAITNGRYENV
                     LAALRQGTSATAVAQAIAEGPWGTHDGVLRVLRGAQGATV"
     gene            3809..>5894
                     /locus_tag="VFX21_04530"
     CDS             3809..>5894
                     /locus_tag="VFX21_04530"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5095251.1"
                     /translation="MATSGSILGNPVRRKEDPGILTGATQYVDDLPVDGLLHVAFVRS
                     TIAHARIEGIDTADALTMPGVVAVYTAGDVGLNAMHGFMMLPPTMNRPPLATDRVRFV
                     GDIVAMVVADSKAHAMDAAEGVIVDYDPLPAVADMDAALTADAPVIHEAHGSNIANAM
                     GTGPVEGVLDDADVVVTAHIENQRLAPVPMEGAGIIVVPGEPAGGATIIVASQGPHGV
                     RDEIAGTLGLDPSVVRARTAAVGGGFGAKQGTMPEHVLVAKAAMMLGRPVKWAETRSE
                     NMVAMWHGRGHRHDVEMGLKRDGTITGLRVHTLADAGAYPGIGAFLPFFTQMMSSNVY
                     AIPKIEFNWQAAVTNTTPVAAYRGAGRPEGIHLTERILDVAADELGIDPVEIRRKNFI
                     QPEAFPYMPNTGLGASYDTGEYEKALDAALAAAGYQELRAEQQARRARNDRMLLGIGV
                     ASYLEISAPMVLTREYGSVTIEDDGSVTARVGTSSHGQGHETAFSQVISAVLGVPFEQ
                     IKVLHSDTDEVRSGGGTGGSRSLQIGGSALHVASTEVLHQAKQLAANVLEANPDDIVV
                     SDGGGLQVAGVPASAMSWADLAQAAKDPAKRPEGVGERLQHELDFDAEGSSFPFGSHV
                     SVVEIDRETGRITMLRHIAVDDCGNIINPLLVAGQQHGGIAQGVAQVLYEAVQYDEDG
                     NPITANLMDYAVP"
BASE COUNT          917 a         1996 c         2055 g          926 t
ORIGIN      
        1 tcgagcgcga agcgcagatg tccgacatcg gaagcatcga agcggcggtc ggtcggcatc
       61 ggcgtcggga aaccccacgc tcgccagagt cgcaacgcgt cctcgagtga tgcgcccgct
      121 cttgctgcga gctctgcgat ggtcagtgac cgatcgggct tgagcagata ctcgaccgtg
      181 agtgagtcga gacggttcga cacgatcgcg tcgtcgatct cctcgcgacg cgcgccgagg
      241 cgggtaagcc gatcgatgat cgcggtgagc gcagcgccgt cgggcggcgg attgccgtgc
      301 ctcacggcag cccgagcacc tgcactgcct cgtcgatcga cgcgacggtg cggttggcct
      361 cggcacacag cgatgcggcg cgcgcaacct gctcttcgtt cgacgggccg gtgtcccagt
      421 cttcgaggcc gaccctgaga tggccgccgc gttccaacgc gagttgcgcg accggcgtgt
      481 cacacactga gccgccgagc accgcaaccg cccacgggat gccggtgttc tcgagcatcg
      541 agacgtagag gtcgagcgct tcgcgcgtcg gcggcgcgcc ccagagtggc tcgccaccgc
      601 cgaggtagcc gccgcccgag aagtagaact tcacgagtgt gcccgcgggc agcttgccgg
      661 cacggtggta cgcgacgacg acgcgcgcga acccgggttc gaagcacgcg atgctcgggc
      721 cgaggccgcg ctcggcgcac accctcatct tgtgcgcgac gtcggagaag gtgttcttgt
      781 agacgtagtc actcgcgggc ggcaggccgt cggggcccgt gccgccgagg ttgaccgaac
      841 cggtgtcgac gaagccggcg cggattaccc cgatgtcgtc gagtagttcg atgtgccggt
      901 agcgctcgtc gatcgtcgcg cccaaccccg tcgtcggata gcagataacg ccggggtgcg
      961 cgtcgacgat cggccggtag gcctcggcgt acgcgagggc cgcgtcttcc ggcggcaccg
     1021 cgatgttggc ggcatgcgtg tggatcaccg tcgcgcccgc gtcgacgcac gcaagaccgt
     1081 ccttcgcctg ttcgtcgggt gcgatcggcg cgtgcggatt gcgcttcttc gacgtcacac
     1141 cgttcaacgc cgcctcaatg atgacaggag tgttcagaga tgccacgagg gcctaggttg
     1201 ccactccagt gcggacggag tgaagccacg gacggcgcaa cgagggagcc atgcggatgc
     1261 agaaccgcgt cacggagatg ttgggcgtcg agtacccgat cgtgcaggcg ccgatgggct
     1321 ggatcgcccg ggcccagctc gcgtcggcgg tgtccaatgc cggtgggctc ggcatcatcg
     1381 agacgtcgtc ggggatgctc gcggaggtgc gcggcgagat tgccaagatg cgcgacctca
     1441 ccgacaagcc gttcggcgtg aacatcgccc agatgttcgt gcgcgatccg agcatcgtcg
     1501 acttcgtcgt cgacaacggc gtgaagttcg tgacgacgtc ggcgggcgac ccgaccaagt
     1561 acacgaagct gttgaaggac gcgggcctca cggtgttcca cgtcgtaccg tcgctgcgcg
     1621 gcgcgctgaa agcggtggat gccggtgtcg acggcctcgt cgtcgaaggc aacgaaggcg
     1681 gcggcttcaa ggcaccgacc ggtgcgtcga cgatggtgct gctgccgctc gtgtgttcca
     1741 aggtcgacgt ccccgtgatc gccgccggcg gcgtgtgcga cggccggtcg atggcagccg
     1801 cgttcgttct gggcgcggag ggtgtgcaga tgggcacccg catggtcagc gcggccgagt
     1861 cgccgatcca cgacaactgg aaacagctca tctgcgcggc cggcgagggc gacacgtact
     1921 tgctcaatcg caacaacccg cccgcgttcc gggtgctcaa gtcggcgttc agtgtgcggg
     1981 ccgagggcgc gggcgacccg gtgatcccga acctcgacaa cgtgctcgac ctctacttca
     2041 acggcaacct cgacgcgtcg ttcgcgttcg gcggccaggt cgcgggccgc atcgacggcg
     2101 tcgaaccggt tgccgacatc ctcgagtcga cgatgcgaga gttcgacgcg acgatgcgag
     2161 aggccgcttc gaggtacgcc tgacgcccag tagcattgcg cgatggggaa ctcggggttc
     2221 gccgtgcgct tcaagctgtt ccgggtcccc gtgacggtgc accccgcatt cttcgtcatc
     2281 ctcttcctgt tcggatacct cggcgccgcc gaatacatct tggtgtgggt tgcgatcggg
     2341 ttcgcgtcga tcctgatcca cgagttcggg cacgcgctca cggcacgtgc gttcgggagc
     2401 cccgcgcgcg tcgagctcgt ctcgagcggc ggcgtgacgt atcacaacca gatgtccgac
     2461 ggtcgcagcc tgctcgtgac gctcgcgggc ccggcgaccg gtatcgcgat cgggcttccg
     2521 ctgtggctcc tcgtcgacga gaccgactac gccggtgccg tgcacgcggc gctcgtgtac
     2581 ggcgtgtgga tcagcctcgc gtggtcgttg ctgaacctct tgccgatcct cccgctcgac
     2641 ggcgggaacg cggtgcagtc gatcctgcaa ctgaccacgg gcaaaccgca tgtcgtcacc
     2701 acgcgccgca tctcgatcgt gatcgcggcc gccgttgccg cgttcgccgc gtcgcagggc
     2761 tacatcttcg gcgcgttcta cgccgtgacg ttcatcggaa tcaatctcag cgctctgcgt
     2821 cagcatcagg aacgcgccaa cgccgccaac cccgccgatc tcaacgccgc ccaacccgtc
     2881 gaccggttgc agcagacggg tgccgcgttg ttggccaagg gcgagatcga ccggggcctc
     2941 gcgttcatca ccgaaggcat cgagcggtcg ggcacgatcg acaagtcggt tgtcgacgtc
     3001 gtgatcgcga cgggaagcac ggaagcgctc gcgacgaagt tgcgccatct tccgcacccg
     3061 cgtggcgggt cgtgggagcc ggtgtttcgc gccgcgctcc agcagctcgg ctacgacgac
     3121 aagcccggca cgttcaacta gacggttgcg ccctgcgcgc cgcgcaggac gcgcagcaca
     3181 ccgtcgtgcg tgccccacgg accttccgcg atggcctgcg cgaccgcggt cgcgctcgtg
     3241 ccctgccgca gcgcggcgag cacattctcg tagcggccgt tggtgatcgc cttgacattt
     3301 gcggcgatgc cgtcctggta gctcgcgtag ttcttcacgc cgaccgagtt caggttcgac
     3361 gtgccggcga agccaccctg cgtcgtggcg agcgggttga agtgggcgcg ggtgccttcg
     3421 gcctgctccc acgcggtgac cgcgcgcacg ttctcggacg acgtcggcat gccgagcgcg
     3481 gtgaggaagt ccttggccca ctgcaccggg tcgacgcccg ctttgttgag gtcgcctccg
     3541 gtcggggcga cgtaggtgtc ggtcgaggtc gccgacgacg ccgaggtcgc ttgcgattcc
     3601 tcagcacggg cgagcgtcga cgcgaagtcg tccgcgtcgc tcgctgcgcc cgcgccgagt
     3661 actccgcccg cgaccgagcc ggccgagaac cggccgacga tctcgttgat tcgcgactgg
     3721 acaccggcga tgccgtccat cggaggtcca atcggccgga agcaggcgct tgttagtgtg
     3781 cggccgcttg cgatttcagg agggcgcagt ggcaacgtcc ggctcgatcc tcggcaaccc
     3841 cgttcgccgc aaggaagacc ccgggatcct caccggtgca acgcagtacg tcgacgacct
     3901 cccggtcgac ggcctgctgc acgtggcctt cgtgcgctcg acgatcgccc acgcccgcat
     3961 cgaggggatc gacaccgccg acgcgctcac gatgcccggc gtcgtggccg tctacacggc
     4021 cggcgatgtc ggcctgaacg cgatgcacgg cttcatgatg ttgccgccga cgatgaaccg
     4081 gccgccgctc gcgaccgacc gcgtgcgctt cgtcggtgac atcgtcgcca tggtcgtcgc
     4141 cgactcgaag gcgcacgcga tggacgcggc cgagggcgtc atcgtcgact acgacccgct
     4201 gcccgcggtc gccgacatgg acgccgcgct cacggccgac gcgcccgtga tccacgaggc
     4261 gcacggatcg aacatcgcga acgcgatggg aaccgggccg gtcgagggcg tgctcgacga
     4321 cgccgacgtc gtcgtcacgg cgcacatcga gaaccagcgc ctcgcgccgg tgccgatgga
     4381 gggcgcgggc atcatcgtcg tgcccggcga accggcgggt ggcgcgacga tcatcgtcgc
     4441 gtcgcagggc ccgcacggcg tacgcgacga gatcgcgggc acgctcggtc tcgatccgtc
     4501 ggtggtgcgg gcgcgcaccg cggcagtcgg cggcgggttc ggcgcgaagc agggcacgat
     4561 gcccgagcac gtgctcgtgg ccaaggccgc gatgatgttg ggccgcccgg tgaagtgggc
     4621 ggagacgcgc tcggagaaca tggtcgcgat gtggcacggc cgcggccacc gccacgacgt
     4681 cgagatgggc ctcaagcgcg acggcacgat caccgggctg cgcgtgcaca cgctcgccga
     4741 cgccggcgcg tatcccggca tcggcgcgtt cctgccgttc ttcacgcaga tgatgtcgtc
     4801 gaacgtgtac gcgatcccga agatcgagtt caactggcag gctgcggtca ccaacacgac
     4861 gccggtcgcg gcatatagag gtgcgggccg gcccgagggc atccacctca ccgagcgcat
     4921 cctcgacgtc gcggccgacg agctcggcat cgacccggtc gagatccggc gtaagaactt
     4981 catccagccc gaggcgttcc cgtacatgcc gaacacgggc ctgggcgcgt cgtacgacac
     5041 gggcgagtac gagaaggcgc tcgacgccgc gctcgcggcc gcgggctacc aggagctgcg
     5101 cgcggagcag caagcacgcc gggcgcgcaa cgaccgcatg cttctcggca tcggcgtcgc
     5161 gtcctatctc gagatcagcg cgcccatggt gctgacgcgc gagtacggct cggtgacgat
     5221 cgaggacgac ggcagcgtca ccgcgcgcgt cggcacgagc tcgcacgggc agggccacga
     5281 gaccgcgttc tcgcaggtga tctcggccgt gctcggcgta ccgttcgagc agatcaaggt
     5341 gttgcactcc gacaccgacg aagtgcggtc gggcggcggc accggcggtt cgcgctcgct
     5401 gcagatcggc ggcagcgcgc tgcacgtcgc cagcaccgag gtgctgcacc aggcgaagca
     5461 gctcgccgcg aacgtgctcg aagcgaaccc cgacgacatc gtcgtcagcg acggcggtgg
     5521 cctccaggtc gcgggcgtgc cggcctcggc gatgtcgtgg gccgacctcg cgcaggcggc
     5581 caaggatccc gccaagcggc ccgagggcgt gggcgagcgc ttgcagcacg agctcgactt
     5641 cgacgccgaa ggctcgtcgt tcccgttcgg atcgcatgtc tcggtcgtcg agatcgaccg
     5701 cgagaccggc cgcatcacca tgctgcgtca catcgcggtc gacgattgcg gcaacatcat
     5761 caacccgttg ctcgtcgccg gccagcagca cggcggcatc gcacaaggtg tcgcgcaggt
     5821 gctctacgaa gccgtgcagt acgacgagga cggcaacccg atcaccgcga acttgatgga
     5881 ctacgcggtg ccgt
//
LOCUS       DASUGA010000231         2944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1141060, whole genome shotgun sequence.
ACCESSION   DASUGA010000231 DASUGA010000000
VERSION     DASUGA010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141060"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VFX21_04535"
     CDS             <1..707
                     /locus_tag="VFX21_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014928328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5095252.1"
                     /translation="ATARRLAADGARVLIADRDVAKGEAVAKELGGLFAQADITDEEQ
                     MTAAVAAAVEMAPLRVCIHCAGVGWVERTVNRDGTPHNLDTFRKIIDIDLIGTFNVMR
                     LAAAAMSKNEAGVDGERGVIVTTASVAAFDGQIGQAAYAAAKGGMVALTLTAARDLSA
                     VGIRVNTIAPGLIDTPLLGGLPDEAREKLAQSVLFPKRLGVPDDFASLAMELVRNHYL
                     NGEVIRMDAGIRMPPK"
     gene            725..1939
                     /locus_tag="VFX21_04540"
     CDS             725..1939
                     /locus_tag="VFX21_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5095253.1"
                     /translation="MPDHPTRDDIDLLEGHFYSDSPGEKYAWLHANAPVYFDAKNNVW
                     GIASYAPLLAAEKDPRTFSNAGGIRPGMGPIPMMIDMDDPEHWKRRKLVNKGFTPRRV
                     RESEPKIRAVCDEIIDSVCERGECDFVNDIAAWLPMILIGDMLGVAPEARGELLQWSD
                     DMLKMQSGSATDEMVMKAMTAMDGYTQFCTHAVAERRANPTDDLMSILVHAELDGDRL
                     ADDAVLHESLLILIGGDETTRHVISGGMEQLLLHPDQHRKLIDDPSLIPRATEEMLRW
                     VSPIKNMCRTVTHDTEFWGRQLSEGQEVLLLFEGANRDPEKFDDPFTFDIGRDPNEHV
                     AFGFGTHYCLGQALARLELVVMFEQLTRRLPDLELATDASSLPRRPANFVSGLESMPV
                     RFTPTAPVGAGA"
     gene            1939..2376
                     /locus_tag="VFX21_04545"
     CDS             1939..2376
                     /locus_tag="VFX21_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5095254.1"
                     /translation="MDNRELVAQFWADLYTRDWDVVAKYFGDDSEYTDVPTPPEDVAR
                     GPAQIVGRLQLGLGVLESIGHDVHTVVCEGDNVVTEHTEHWAWNTGERAALPFVSMQQ
                     VRDGKIVRWWDYWDMGTLMNSAPAWWIEHVMAGSTELGLRGDD"
     gene            2447..2815
                     /locus_tag="VFX21_04550"
     CDS             2447..2815
                     /locus_tag="VFX21_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5095255.1"
                     /translation="MGEHAGYHEPLESLDAATMDRHRAIVSLQEELEAVDWYDQRVAA
                     ATDDDLRAVLAHNRDEEKEHAAMTLEWLRRQDAKWDEVLRTYLFTDAPITEIEAIVEG
                     ASSGERATPSIGDLRGAPEP"
     gene            2812..>2944
                     /locus_tag="VFX21_04555"
     CDS             2812..>2944
                     /locus_tag="VFX21_04555"
                     /inference="COORDINATES: protein motif:HMM:NF016349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="encapsulin"
                     /protein_id="HEX5095256.1"
                     /translation="MNHLLRELAPVPDDAWEQVTEEATRTLTHFLVARRLVDFSGPHG
                     "
BASE COUNT          536 a         1005 c          979 g          424 t
ORIGIN      
        1 aggccacggc tcgccgcctt gccgccgacg gcgcccgcgt cctcatcgcc gaccgcgacg
       61 tcgccaaggg cgaggccgtc gccaaggagc tcggcggcct cttcgcgcaa gccgacatca
      121 ccgacgagga acagatgacc gcggcggtcg cggccgcggt cgagatggcg ccgttgcgcg
      181 tgtgcatcca ctgcgccggt gtcggctggg tcgaacgcac ggtgaatcgc gacggcacgc
      241 cgcacaacct cgacacgttc cgcaagatca tcgacatcga cttgatcggc acgttcaacg
      301 tgatgcggct cgcggccgcg gcgatgagca agaacgaagc gggcgtcgac ggcgaacgcg
      361 gcgtcatcgt cacgaccgcg tcggtcgccg cgttcgacgg ccagatcggg caagccgcgt
      421 acgcggccgc gaagggcggc atggtcgcgt tgacgctcac cgcggcgcgc gacctgtccg
      481 cggtcggcat ccgcgtgaac acgatcgcac ccggtctcat cgacacgccg ctgctcggcg
      541 ggctgcccga cgaagcgcgc gagaagctgg cccagagcgt gctgttcccg aagcggctcg
      601 gcgtgcccga cgacttcgcg tcgctcgcga tggagctcgt gcgcaaccac tacctgaacg
      661 gcgaagtgat caggatggac gccggcattc gcatgccgcc caagtaagcg gaggaccggc
      721 ccgcatgccc gaccacccga ctcgcgacga catcgacctc ctcgagggcc acttctactc
      781 cgacagtccg ggtgagaagt acgcgtggct gcacgcgaac gcgcccgtct acttcgacgc
      841 caagaacaac gtgtggggta tcgcgtcgta cgcgccactg ctcgcggccg aaaaggaccc
      901 gcgcaccttc tcgaacgcgg gcggcatccg cccgggcatg ggtcccatcc cgatgatgat
      961 cgacatggac gatcccgagc actggaagcg ccggaagctc gtcaacaagg gcttcacgcc
     1021 gaggcgcgtg cgcgagagcg aaccgaagat ccgcgcggtg tgcgacgaga tcatcgacag
     1081 cgtctgcgag cgcggcgagt gcgacttcgt gaacgacatc gcggcgtggc tgccgatgat
     1141 cctcatcggc gacatgctcg gcgtcgcgcc cgaagcacgc ggcgagctct tgcagtggtc
     1201 cgacgacatg ctgaagatgc agagcggcag cgccaccgac gagatggtca tgaaggcgat
     1261 gaccgcgatg gacggctata cgcagttctg cacgcacgcg gtcgccgagc gccgggcgaa
     1321 cccgaccgac gacttgatga gcatcctcgt gcacgcggag ctcgacggcg accgtctcgc
     1381 ggacgacgcg gtgttgcacg agtcgctgct catcctcatc ggcggcgacg agacgacgcg
     1441 ccacgtcatc agcggcggca tggaacagct gctgctgcac cccgaccagc accgcaagct
     1501 catcgacgac ccctcgctga tcccgcgcgc caccgaagag atgctgcgct gggtgtcgcc
     1561 gatcaagaac atgtgtcgca cggtgaccca cgacaccgag ttctggggcc ggcaactctc
     1621 cgaaggtcaa gaggtgctct tgctgttcga aggcgcgaac cgcgaccccg agaagttcga
     1681 cgacccgttc acgttcgaca tcggccgcga tcccaacgag cacgtcgcat tcggcttcgg
     1741 cacgcattac tgcctcggtc aggcgctcgc ccgcctcgag ctcgtcgtga tgttcgagca
     1801 actcacgcgg cgcctccccg acctcgagct ggccaccgat gcgtcgtcgt tgccgcgccg
     1861 tcccgcgaac ttcgtgagcg gcctcgagtc gatgcccgtg cgcttcacgc cgaccgcgcc
     1921 cgtcggggca ggtgcgtgat ggacaaccgc gagctggtcg cgcagttctg ggccgatctc
     1981 tacacgcgtg actgggacgt cgtcgcgaag tacttcggtg acgacagcga atacaccgac
     2041 gtaccgacac cacccgagga cgttgcgcgc gggccggcac agatcgtcgg ccggttgcag
     2101 ctcgggctcg gcgtgctcga gtcgatcggc cacgacgtgc acaccgtcgt gtgcgaaggc
     2161 gacaacgttg ttaccgagca caccgagcac tgggcgtgga acaccggcga gcgcgccgcg
     2221 ttgccgttcg tgtcgatgca gcaggtgcgc gacggcaaga tcgttcgctg gtgggattac
     2281 tgggacatgg ggacgttgat gaacagcgct ccggcctggt ggatcgagca cgtgatggcg
     2341 ggaagcaccg agctcggtct gcgcggagac gactgaaccg cggaacaaac ccgccgtttg
     2401 gggacatgcg cctgcgggta cttcgggtcg accgggaagg acacgcatgg gcgaacacgc
     2461 gggataccac gaaccactcg agtcgctcga cgcagccacg atggatcgcc accgcgccat
     2521 cgtgtcgctc caagaagagc tcgaagccgt cgactggtac gaccagcgcg tcgcggccgc
     2581 gacggacgac gacctgcgcg cggtcctcgc gcacaaccgc gacgaagaga aggaacacgc
     2641 ggcgatgacg ctcgagtggt tgcgtcgcca ggatgcgaag tgggacgaag tcctgcgcac
     2701 ctacttgttc actgacgcgc ccatcaccga gatcgaagca atcgtcgaag gcgcgtcgtc
     2761 cggcgaacgc gctacgccct ccattggcga cctccgagga gcacccgagc catgaaccac
     2821 ctgctgcgcg agctggcgcc cgtccccgac gacgcgtggg agcaggtcac cgaagaggcg
     2881 acgcgaacgc tcacgcactt tctcgtggca cgccgcctcg tcgacttctc gggtccgcac
     2941 ggct
//
LOCUS       DASUGA010000232         2855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1679288, whole genome shotgun sequence.
ACCESSION   DASUGA010000232 DASUGA010000000
VERSION     DASUGA010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1679288"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="VFX21_04560"
     CDS             <1..977
                     /locus_tag="VFX21_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEX5095257.1"
                     /translation="LRLGLHTLGDVAALPVDAVLGRFGLEGCRYHDLARGIDPGPPVL
                     VPPPPDLVEQTELDPPAERVDMAAFAAKELADRLVERLADRGLACTRVLVEAETEHGE
                     VLTRCWRHAEGLTPGALAERVRWQLDGWISTGETTAGIAVLRLVPDEVVPADGRQLGF
                     WGGDQVAHDRADRALARVQGMLGFDAVATAVVQGGRTPAEQVRWVPWGEPRDEDRQAR
                     AAWPGSLPAPYPARVFDPPIAAELVDAHGNPIAVSGRGEQMAAPAHVHSRIVSGDVAG
                     WAGPWAHDVRWWDPEARDRCARYQIMVGDTACLVVLEGGRTAIEAIYD"
     gene            1099..1317
                     /locus_tag="VFX21_04565"
     CDS             1099..1317
                     /locus_tag="VFX21_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095258.1"
                     /translation="MPDRRADRWTIMFFVTFALLLAVALILAAIGNRDAAVIFAFAAV
                     STTLVLRVRLQVLARRELGREREERGER"
     gene            1381..1797
                     /locus_tag="VFX21_04570"
     CDS             1381..1797
                     /locus_tag="VFX21_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095259.1"
                     /translation="MRRCLIVANQTLTGPALMRAVVDRQVREPCDFHIVVPATHQHGT
                     AAWTEGGALAHARSTLQHALEQFGDAGIPATGEVGDASPMLAVGDVLRREHIDEIILS
                     TLPPGASKWLKRDLPHRLARYHLPVTHIVADPVPVA"
     gene            1818..2597
                     /locus_tag="VFX21_04575"
     CDS             1818..2597
                     /locus_tag="VFX21_04575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEX5095260.1"
                     /translation="MATDAAWTDLLHALDAQHAELAAIVDQCTGDDWERPTRCEGWDV
                     ADVLLHLAATDEFANWTALGKLGNDEHGFLSDGDRQTVTVDDAAAAQVASDRAAGGAA
                     IRERWHAASEAMRATFNAGDPHARVRWVSGRLSLHTLTATRLSECWIHTGDIALALGI
                     DLPPTNRLQYIARLAWRTLPYAFARDNKEMAGPVALVLTGPDADTWYFEPDAPALTTI
                     RGKAEEFCDVAARRVEPSATTLEGEGPDVENVLALVRTYAL"
     gene            2650..>2855
                     /locus_tag="VFX21_04580"
     CDS             2650..>2855
                     /locus_tag="VFX21_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5095261.1"
                     /translation="MATFVLIHGAGDVGWYWHLVADELRVAQHDVVAPDLPCEDDAAG
                     LVEYADAVIDAIGDRKDLVVVGQSL"
BASE COUNT          431 a          941 c         1009 g          474 t
ORIGIN      
        1 tgttgcgttt gggattgcac acgttgggcg acgtcgcggc gttgccggtc gacgccgtgc
       61 tcggccgctt cgggcttgaa ggctgccgct atcacgactt ggcgcgtggc atcgatcccg
      121 gcccgccggt actggtgccg ccgccgcccg acctcgtgga gcagacggag ctcgatccgc
      181 ccgccgaacg ggtcgacatg gctgcgttcg cggcgaagga gctcgccgac cggttggttg
      241 aacggctggc cgatcgcggg ttggcgtgca cgcgcgttct cgtcgaagcc gagaccgagc
      301 acggcgaggt gctgacgcgt tgttggcgtc atgccgaagg actgacgccg ggcgcgttgg
      361 cggagcgtgt gcgttggcaa ctcgacggct ggatctccac tggtgaaacg acggcgggaa
      421 ttgccgtgct ccgactcgtc cctgacgaag tggtgccggc ggatgggcgc cagttgggat
      481 tctggggcgg cgaccaagtc gcgcacgacc gcgccgatcg cgcgttggcg cgcgtgcaag
      541 ggatgttggg gttcgacgcg gtcgcgaccg cggtcgtgca aggcggccgt actcctgcgg
      601 aacaagtgcg gtgggtgccg tggggcgagc cgcgcgacga agaccggcaa gcgagagccg
      661 cgtggcccgg ttcattgccc gcgccgtatc cggcccgcgt gttcgatccg ccgatcgcgg
      721 ccgagctcgt cgatgcgcac ggcaatccga ttgcggtgag cggccgcggc gaacagatgg
      781 cggcccctgc gcatgtgcac agccgcatcg tgagcggcga tgtcgctggc tgggccggac
      841 cgtgggcgca cgacgtgcgc tggtgggacc ccgaagcccg cgaccgctgc gcccgctacc
      901 aaatcatggt cggcgacacc gcgtgtctcg tcgtgctgga aggcggccgc accgccatcg
      961 aagccatcta cgactgattc tgtgtgacac tgccggtgtg gcggagcgct ggatgcggat
     1021 tccggttggg tgcttggtcg ccgcgaccgc cctttcggca agcgcgctga acggctgcag
     1081 tgacgttgac gatccctgat gcctgatcgt cgtgcagacc gttggacaat catgttcttt
     1141 gtcacgttcg ccttgttgct cgcggtcgcg ctcatcctcg ccgccattgg caaccgggac
     1201 gcggcggtga ttttcgcgtt cgcggcggta tccacaaccc tggtccttcg tgttcgtttg
     1261 caagtactcg cccgccgtga actcggtcgc gagcgcgaag agcgcggcga gcgatagaac
     1321 ctctttgcgc cgggaagaat cggcgcgaca atcgaagccc cacctgcctt ggaggtgccc
     1381 atgcggcgtt gcctcatcgt ggcgaaccag acgctcaccg gcccggcgtt gatgagggcg
     1441 gtggtcgacc gccaagtgcg ggagccgtgc gacttccaca tcgtggtgcc ggcgacgcat
     1501 cagcacggca ccgcggcgtg gaccgaaggg ggcgcgctcg cccatgcccg gtcgacgttg
     1561 caacacgcgc tcgagcagtt cggcgacgcc ggcattcccg cgacgggcga ggtcggcgac
     1621 gcgagcccga tgcttgcggt cggcgacgtc ctccgccgcg agcacatcga cgagatcatt
     1681 ctgtcgacgc tgccgccggg tgcgtcgaag tggctgaagc gtgacctgcc gcaccggctc
     1741 gcgcgctatc acttgcccgt gacccacatc gtcgccgatc ctgttccggt cgcatagtcc
     1801 gtcgaacgga gaaccgggtg gcaaccgacg ccgcgtggac cgatctgctg cacgcgctcg
     1861 acgcgcagca cgccgaactc gccgcgatcg tcgatcagtg cactggcgac gactgggagc
     1921 gaccgacgcg ctgcgagggc tgggatgttg ccgacgtgct gttgcacctc gccgcgaccg
     1981 acgagttcgc gaactggacc gcgctcggaa agctcggcaa cgacgagcac ggcttcctga
     2041 gcgacggaga tcgccagacg gttaccgtcg acgacgcggc ggccgcgcag gtcgcatcgg
     2101 atcgagccgc aggtggcgca gcgatccgcg agaggtggca tgccgcgtcg gaagcgatgc
     2161 gagcgacgtt caacgccggc gatccgcacg cgcgcgtgcg gtgggtatcg ggcaggttgt
     2221 ccctgcacac gctgacggcg acgcgactct cggagtgctg gatccacacc ggagacatcg
     2281 cacttgcgct cggcatcgac ctgccgccta ccaaccggct gcagtacatc gcccggctcg
     2341 cgtggcgcac cctcccgtac gcgttcgcgc gcgacaacaa ggagatggcc gggccggtcg
     2401 cgctcgtgct caccgggccg gacgcggaca cgtggtactt cgaaccggac gcgcccgcgc
     2461 tgaccacgat ccggggcaag gccgaggagt tctgcgacgt cgcagcccgc cgcgtcgaac
     2521 cgagcgcaac cacgctcgaa ggtgaaggcc ctgacgtcga aaacgtcttg gctctcgtcc
     2581 gcacgtacgc gctgtagccc ctcgccgaac aagacgttcg acgcttgcgc tccgcgggta
     2641 ccaaccggcg tggccacgtt cgtcctgatt cacggcgcag gtgatgtcgg ttggtattgg
     2701 catctcgttg ccgacgagct gcgcgtggcg cagcacgatg tcgtcgcgcc cgatcttcca
     2761 tgcgaagacg acgccgccgg tctcgtcgag tacgccgatg ctgtgatcga cgcgatcggc
     2821 gatcgaaagg atctggtcgt cgtcggccag tcgct
//
LOCUS       DASUGA010000233         3103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_463972, whole genome shotgun sequence.
ACCESSION   DASUGA010000233 DASUGA010000000
VERSION     DASUGA010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463972"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..842
                     /locus_tag="VFX21_04585"
     CDS             132..842
                     /locus_tag="VFX21_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095262.1"
                     /translation="MTPEQAAHETASAIVSTSSRFMTDPATYGDAAKLGFEGMDFYIA
                     GRGGVLGEVPAAVATAALVFFEPAAVSAAWVRSGSLMSRAEAAATWADAMHHWARAHF
                     ADDVDWARVAELAGEIVRAAPVAGAPLFAGWRVLPEPGDAKALAMHRMNALRELRGAL
                     HEAAILTVGLTPLEAIAVRSPRMASVFGWTEALPDTAPLLDRWQLAESRTDRMIGRHY
                     RALTESAREEFVTLLSNS"
     gene            complement(877..1134)
                     /locus_tag="VFX21_04590"
     CDS             complement(877..1134)
                     /locus_tag="VFX21_04590"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX5095263.1"
                     /translation="MPTPQRQFPVGSYAIDFAVPDRMVGVECEGFEFHGNRLQWKRDK
                     RRTAFLERREWRLVFVTWEDVTRYPNQTLDRIAIALRRAAA"
     gene            1865..2329
                     /locus_tag="VFX21_04595"
     CDS             1865..2329
                     /locus_tag="VFX21_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HEX5095264.1"
                     /translation="MTMLEIPTAVHRGNDELPWVDIGGGSLLKVLQVKEREGLWVILN
                     RFEAGYDVQTHKHTGPVYAFTFSGSWKYKESDFVNRAGSFLYEPAGSVHTLQVPADNT
                     EPTEVFFAIWGANLNLDADGNVESVIDAGGILQAYYMMAEAEGHPRPNVILD"
     gene            2340..>3103
                     /locus_tag="VFX21_04600"
     CDS             2340..>3103
                     /locus_tag="VFX21_04600"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5095265.1"
                     /translation="MSILGTRVLRSEDPRFLTDGGNYIESIDLPGAAHVVYVQSTVAH
                     GRITSIDTTDAARMPGVIGVFTAADVDLAPQDRPNRRVPEPMARPLLATDTVRFVGEL
                     IGAVVAETRTQAVDAAEMVVVDIDPLPAVVDTEVALRNEALLYPDAETNTAFVFPSRA
                     GDDFFDGCEVVIEQRIVNQRVAPCPLEVRAAAARWGPDGRLTQWACTQGPHGTRNDLA
                     KVLGLERDQVRVITPDVGGGFGAKFGAYPEEVLVPWL"
BASE COUNT          495 a         1065 c         1043 g          500 t
ORIGIN      
        1 gaggaagccg tctacgcgaa caacaccgag gcgacgctcg ccgcgttgac cgcgggtcgc
       61 gacgaccggc cgtcggccga tgacgtgttg tcggccgcgg ttggcgaacc gcagaacccg
      121 tacttccgta catgactccc gaacaggccg cccacgagac cgcgagcgcc atcgtctcga
      181 cgtcgtcgcg tttcatgacc gatcccgcga cgtacggcga tgccgcgaag ctcggcttcg
      241 aaggcatgga cttctacatc gcgggccgcg gcggcgtgct gggtgaggtc ccggccgcgg
      301 tggccaccgc cgccctcgtg ttcttcgagc ccgccgccgt ctcggccgcg tgggtgcggt
      361 ccggttcgtt gatgtcgcgc gcggaagcgg ccgcgacatg ggccgacgcc atgcaccact
      421 gggcccgcgc ccacttcgcc gacgacgtcg actgggcgcg cgtcgccgag ctcgcgggtg
      481 agatcgtccg cgccgcaccg gtggccggtg cgccgttgtt cgccggctgg cgcgtgctgc
      541 ccgaaccggg cgacgccaag gcgctcgcca tgcaccgcat gaacgcgctg cgcgagctgc
      601 gcggcgcgct gcacgaagcc gcgatcctga cggtcggtct gacgccgctc gaagcgatcg
      661 cagtgcgcag cccacggatg gcgtcggtgt tcggctggac cgaagcgctc cccgacaccg
      721 cgcccctcct cgaccgctgg cagctcgccg aatcgagaac cgaccgtatg atcggccgcc
      781 actaccgcgc gctcacggag tcagcccgcg aagagttcgt caccctcctg tcgaactcat
      841 gagctggacc gccatatttg gcggtctggc tcatagctac gcagctgcgc ggcgaagtgc
      901 gatcgcgatg cggtcgagcg tctggttcgg atagcgcgtg acgtcctccc aagtgacgaa
      961 cacaagtcgc cattcgcgcc gttcgaggaa cgcggtgcgg cgcttgtcgc gcttccattg
     1021 cagtcggttg ccatggaact cgaatccttc gcactcgacg ccgaccattc ggtcgggcac
     1081 cgcaaagtcg atggcatagg acccgacggg gaactggcgc tgcggtgtcg gcaacgcgct
     1141 cgtgcgccag agccgcgccc ttcacttcga gcttgaactg aagggcgggc tgcccgtctt
     1201 gatgcgacag cagttcacga atggccttcg tcccggcctt cccgcgtccg cacagatctt
     1261 cggcgcgccg ggcgagcagt gcgggtgtgg tgagtcgcat acggcgtgcc gcttcgaacg
     1321 cgacttcgag tggttcaccg ctcatcatcg acgccagatc aatgatcgtg cgcgtcgcgc
     1381 tcaagcacgg gatgtcgtcg acgaacactc gatcgacggg ttgcagcgat gccgctctgt
     1441 gcagcacggc cgagtcggtc cgcagtccgg agtcgctcgg gaccgtgagg tgcagctcct
     1501 tgggccggat gccgtccaac cgcagtaacc gaccggcagt tgtgtgtgac accgcgctgt
     1561 cggtgccggc ccacagcgtc gcggcatacg ctcgttggcg gcccgtcctc ggtgcgccac
     1621 cgagtcgata gacgtggtgg aacagctctt cccaagcgcc ggactcgacg cgccgctgaa
     1681 tcgtgcggtc cgtcatgccc cacgcgaggg cttgcgagtg gctgacgacc ccataacgtt
     1741 cggccgccat tgccgcgacc atccgttctg caccgttcat atgagctacc tcgccacata
     1801 tggcggtttg gatcatgaga acggggaagg ccgggagaag tggggcgaga gtatcgtccc
     1861 gattatgacg atgctcgaga ttcctactgc ggtgcaccgc ggcaatgacg agttgccgtg
     1921 ggtcgacatc ggcggcggtt cgctgttgaa agtgctccag gtgaaggagc gcgaggggtt
     1981 gtgggtgatc ctcaaccgct tcgaggccgg ctacgacgtg cagacgcaca agcacacagg
     2041 tccggtgtac gcgttcacgt tctcgggctc gtggaagtac aaggaatccg acttcgtgaa
     2101 ccgcgccggg tcgttcctgt acgagcccgc gggttcggtg cacacgctgc aggtgccggc
     2161 cgacaacacc gagccgaccg aggtgttctt cgcgatctgg ggcgcgaacc tcaacctcga
     2221 cgcggacggc aacgtcgaga gcgtcatcga cgccggcggc atcctgcagg cgtactacat
     2281 gatggccgag gccgaagggc acccgcgccc gaacgtcatc ctcgactgac gtccacgcag
     2341 tgagcattct cggaacgcgc gtcctgcgca gtgaggaccc gcgcttcctc accgacggcg
     2401 gcaactacat cgagagcatc gacctgcccg gtgccgcgca cgtcgtctac gtgcagtcga
     2461 cggtggcgca cggccgcatc acgagcatcg acaccacgga cgcggcgagg atgccgggcg
     2521 tcatcggcgt gttcaccgcg gccgacgtcg acctcgcgcc gcaggaccgg ccgaacaggc
     2581 gcgtccccga gccgatggca cggccgctgc tcgcgacaga caccgtccgt ttcgtcggcg
     2641 aactcatcgg ggcggtcgtc gcggagacgc gcacccaggc cgtcgacgcc gcggagatgg
     2701 tcgtcgtcga catcgatccg ctccccgccg tcgtcgacac cgaagtcgcg ctccgcaacg
     2761 aagcgctgct ctaccccgac gccgagacga acaccgcgtt cgtgttcccg tcgcgcgccg
     2821 gcgacgactt cttcgacggc tgtgaggtgg tcatcgagca gcgcatcgtg aaccaacgcg
     2881 tcgcgccgtg tccgctcgaa gtgcgcgccg cggccgcgcg ctggggaccc gacggccgtc
     2941 tcacgcagtg ggcgtgcacg caagggccgc acggcacccg caacgacctc gccaaggtgc
     3001 tggggctcga acgcgaccag gtccgcgtga tcacgcccga cgtcgggggc ggcttcggtg
     3061 cgaagttcgg cgcctatccc gaggaagtgc tcgtcccgtg gct
//
LOCUS       DASUGA010000234         3904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_386492, whole genome shotgun sequence.
ACCESSION   DASUGA010000234 DASUGA010000000
VERSION     DASUGA010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386492"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            510..1523
                     /gene="moaA"
                     /locus_tag="VFX21_04605"
     CDS             510..1523
                     /gene="moaA"
                     /locus_tag="VFX21_04605"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX5095266.1"
                     /translation="MVRRAIPLIDPHARRVKDLRISITDRCNFRCAYCMPAEGMDWLP
                     RTELLTYEEIARVARVCVEQFGFEAIRLTGGEPLVRAHITRLVGMLAALDVDLALTTN
                     GVKLPEMAHDLAASGLQRVNVSLDSLRRETFLALTRRDELDRVIAGIDAARHAGLSPV
                     KVNAVVMRGVNDDEVVDLARFAREREIGMRFIEFMPLDAGDEWSADKVVPADEILERI
                     DAVFPLDRVTDDHVDPAARYAYRDGHGDVGVIASVTEPFCESCDRVRITAEGQFRTCL
                     FALEEFDLRAELRAGAGLDEPDLDDRLAAAITRAVGTKWAGHHIGQADFVRPSRSMSQ
                     IGG"
     gene            1607..2107
                     /gene="moaC"
                     /locus_tag="VFX21_04610"
     CDS             1607..2107
                     /gene="moaC"
                     /locus_tag="VFX21_04610"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX5095267.1"
                     /translation="MPSEFTHLDPLGRARMVDVTAKEPSHRRAVARCRVNMEAETASK
                     IATGAITKGDVLAVARVAGIQAAKQTPNLLPLCHPLLVGAVLVNFRIEDTYVEVEANV
                     DTVDRTGVEMEAMTACAVAALTIYDMCKSIDRGMTIGDLALWEKTGGRSGTFRRAASD
                     GDPFAL"
     gene            2499..2717
                     /locus_tag="VFX21_04615"
     CDS             2499..2717
                     /locus_tag="VFX21_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095268.1"
                     /translation="MLIGAVLMTLLVAQVSGAKAVWALQLVADVALIAYVGLWAYFRS
                     LQADRAAKVHALPRRRAPELALRRTGSS"
     gene            2723..3364
                     /locus_tag="VFX21_04620"
     CDS             2723..3364
                     /locus_tag="VFX21_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HEX5095269.1"
                     /translation="MDRAAEIAALGARARDAFDDKHRAREITIAASRRAIQACAASIR
                     ATHRSEFDAAEKLAREARDHLDEADGAVHNFPDVRTNGPIYDAKKELAEAWLTLALVR
                     EEPLPTPTDLRVDVAPYCNGLAEAASELRRQLLDQLRAGDLSRAERLMRSMDEVYSIL
                     VTIDYPDGVTGGLRRTTDQLRAVLERTRGDLTTAMVAARLQSAIESARDATRD"
     gene            3450..>3904
                     /locus_tag="VFX21_04625"
     CDS             3450..>3904
                     /locus_tag="VFX21_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095270.1"
                     /translation="METSGATGSRRDHAVALGIALGALVLIVGLIAAVGSNDDATSGV
                     VAGAPVATENVADVSDSGGGSDGASNADVTVTTMGAMEHDHGSGASGATTDEHDMEHD
                     SSEHAHTDATTGSHDAQHAHDSTSATSAGEHDHGDAPTTPGATSAGAHE"
BASE COUNT          621 a         1328 c         1341 g          614 t
ORIGIN      
        1 agcggtccgt cgctggtccg gatatgtggc gaaggggtca cggacccgcg gatgagcggg
       61 cgccggaggg gggacaccag cgcccgcatc cgcagcgtgc cgctcgtcgt tgagcaaccc
      121 gatcacggct gagcgatccg cctacctcgc gatactgcgc gcgtagagtg gttagagcaa
      181 gacgttgagc gcgagtgtgg accacgtcac cttcggcacc gaccgcttca ccgaccgttg
      241 tcatggctca cgaagcgtgg tacaacgtgc tggcttcgga acttcgcccg ggcgggcgac
      301 accgacagca tcgggaggct gcgcaaaacc gtggcaccga ccgaaggcgc gtgggctggc
      361 gcgactgcgg tcacctggag aggcatagat ccgtcggctg gagttggtcg ctgcgccgtc
      421 ggggagccag agcgaacccg cctccaggcg ccacagggac ggaaccccgc cggggcgacc
      481 cccgattcct ctgcccgtaa ccttggtgca tggttcggcg cgcgattcct ctcatcgatc
      541 cgcacgcgcg gcgcgtgaag gacctgcgga tctcgatcac cgatcggtgc aacttccggt
      601 gcgcctactg catgccggcc gaggggatgg actggctgcc ccgcaccgag ctcctcacct
      661 acgaggagat cgcccgggta gcccgcgtgt gcgtcgagca gttcggcttc gaggcgatca
      721 ggctcaccgg cggcgagccg ttggtgcggg cccacatcac gcggctcgtc ggcatgctcg
      781 ccgcgctcga cgtcgacctc gcgctgacga cgaacggcgt gaagctgccc gagatggcgc
      841 acgacctcgc ggcgtcgggt ctgcagcgag tgaacgtctc gctcgattcc ttgcgccgcg
      901 aaacgttcct cgcgttgacg cgtcgcgacg agctcgatcg cgtgatcgcg ggcatcgacg
      961 cggcgcgcca cgcggggctg tcaccggtca aggtcaacgc ggtcgtgatg cgcggcgtca
     1021 acgacgacga ggttgtcgac ttggcgcgct tcgcgcgcga acgcgagatc gggatgcgct
     1081 tcatcgagtt catgccgctc gatgcaggcg acgagtggag cgccgacaag gtcgtccccg
     1141 ccgacgagat tctcgaacga attgacgctg ttttcccgct cgatcgcgtc accgacgacc
     1201 acgtcgatcc cgccgcgcgc tacgcgtatc gcgacggtca cggcgatgtc ggcgtgatcg
     1261 ccagcgtgac cgaaccgttc tgcgagagct gcgaccgcgt ccgcatcacc gcggaaggac
     1321 agttccgcac gtgtctgttc gcgctcgagg agttcgacct gcgcgccgag ctccgcgccg
     1381 gcgccgggct cgacgagccc gatctcgacg accggctcgc ggccgcgatc acgcgcgccg
     1441 tcggcaccaa gtgggcgggc catcacatcg gccaggccga cttcgtgcgg ccgtctcggt
     1501 cgatgagtca gatcggcggt tgaggaccgg cgccgccgcc ccggagtcgg gccggcccgc
     1561 ccgtcccggt cgagatcccg ccgggtcgcc tctagactcg gcccccatgc cctcggaatt
     1621 cacgcacctc gacccgctgg gtcgcgcccg gatggtcgac gtcaccgcga aggagccctc
     1681 gcaccggcgg gccgtcgctc gttgccgcgt gaacatggag gcggagacgg cgtccaagat
     1741 cgcaaccggc gcgatcacaa agggcgacgt gctcgcggtc gcgcgcgtcg cgggcatcca
     1801 agcggcgaag caaacgccga acttgttgcc gctgtgtcac ccgttgctcg tcggcgcggt
     1861 gctcgtgaac ttccgcatcg aagacacgta cgtcgaagtg gaagcgaatg tcgacacggt
     1921 cgaccgcacc ggtgtcgaga tggaagcgat gaccgcgtgt gcagttgccg cgctcacgat
     1981 ctacgacatg tgcaaatcga tcgatcgcgg catgaccatc ggtgacctcg cgttgtggga
     2041 gaaaacgggc ggtcgctccg gaacgtttcg ccgggcggca agcgatggag atccgttcgc
     2101 gctctgagtg agccgcaatg cgtgtcacct ggtgttttac ggggtagacg gttcgcgcgt
     2161 cgggtgtggt tggcaataca cattcgtacg acgcagactt cttatgtgcg ttcgggtcca
     2221 ccacctgaac ttcacgcgtt gttaccgtcg tctacagcaa aggcggtcga ggggtcttgc
     2281 cgttcgtgtt ggtcttgatt gtgctcggag cactgtgggg gatcgtgttg gtcccgccgt
     2341 tgcttcgttc ccgcaccgag cggacctccg attcgatcgg cgacttcaat caccgcctcg
     2401 gtgtcctcag tcgcaccaac gacacggggc gaacgtcgcg tcctgtctcg tctcggcgtc
     2461 caccttcggc ggcgaagcgc cggcgtgacg tctgcaccgt gctcatcggc gcggtgctca
     2521 tgacgttgct cgtcgcgcag gtgtccggtg caaaggcggt gtgggcgctg caactggtcg
     2581 ccgacgtcgc gctcatcgcg tacgtcggtc tgtgggcgta cttccggtcg ctgcaggccg
     2641 accgtgccgc gaaggtgcac gcgttgccac gacgtcgcgc gccggagttg gcactgcgcc
     2701 gcaccggttc ttcgtagtcg cgatggatcg cgctgccgag atcgccgcgc tgggcgctcg
     2761 cgctcgcgac gccttcgacg acaagcaccg tgcgcgagag atcacgatcg cggcgtcgcg
     2821 acgcgcgatc caagcgtgtg ccgcgtcgat tcgcgccacg catcgcagcg agttcgatgc
     2881 tgccgagaag ctcgcgcgag aagcgcgcga ccatctcgac gaagccgacg gcgcggtgca
     2941 caacttcccc gacgtgcgta cgaacgggcc gatctacgac gcgaagaagg agctcgccga
     3001 ggcctggctg acgctcgcgc tcgtgcgcga ggaaccgttg ccgacgccca ccgatctccg
     3061 ggtcgacgtc gccccgtact gcaacgggct ggccgaggcc gcgagcgagc tgcggcgcca
     3121 gctgctcgac cagctccggg ccggcgacct ctcccgggcc gagcgattga tgcggtcgat
     3181 ggacgaggtg tattcgatcc tcgtgaccat cgactacccg gacggtgtga ccggcggttt
     3241 gcggcgcacc accgaccagc tgcgggccgt cctcgagcgc acacggggcg acctgacgac
     3301 ggccatggtc gcggcccgcc tccagagcgc catcgaatcg gcgcgcgacg cgacgcgcga
     3361 ctgacacaca ctcagcgcgg ccgaaacttc acgaaaccct gatcgttcgt tggtggcccc
     3421 cggccacacc cgttgctaca caacgtccga tggagacctc aggggcaacc ggaagccgcc
     3481 gcgaccatgc cgtcgcgctc ggcatcgcac tcggcgcgct cgtccttatc gtcggtttga
     3541 tcgcggcggt cggttcgaac gacgacgcca cctcgggggt ggtggccggt gcgccggtcg
     3601 cgaccgagaa cgttgccgac gtctcagatt ccggcggcgg gtcggacgga gcgtcgaacg
     3661 cggacgtgac ggtcacgacg atgggcgcga tggaacacga ccacggctcc ggcgcgtcgg
     3721 gtgcgacgac cgacgagcac gacatggagc acgactcgtc cgagcacgcg cacaccgacg
     3781 cgacgaccgg cagccacgac gcccagcacg cgcacgacag cacgagcgcg acgtccgccg
     3841 gcgagcacga ccacggcgac gcgccgacca cgccgggcgc gacgagtgcc ggcgcgcacg
     3901 agca
//
LOCUS       DASUGA010000235         2853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_898015, whole genome shotgun sequence.
ACCESSION   DASUGA010000235 DASUGA010000000
VERSION     DASUGA010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898015"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2249
                     /locus_tag="VFX21_04630"
     CDS             <1..2249
                     /locus_tag="VFX21_04630"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEX5095271.1"
                     /translation="SNIRSSYEHLAGGGTASGPVSFMRGADASAGTIKSGGKTRRAAK
                     MVILNADHPDIEEFVWCKAVEERKARALAEAGFDMDLDGKDSHSIQYQNANNSVRVTD
                     EFMQAVVDDRDWALRAVTTGEPLKTLQARDLWRQIAESAWECADPGMQFDTTINRWHT
                     AAVTGRINASNPCSEYMHLDNSACNLASINLLKYLGDDGAFDVDGFKHTVDVVFTAQE
                     ILVGNADYPTEPIADTSRKFRQLGLGYANLGALLMAQGMPYDSDAGRAWAAAITALMT
                     GQAYATSARTAARMGPFAGYADNAEPMLNVLRMHRAEAARIDEDIVPPELLGAAQQSW
                     DEAVELGERHGVRNSQASVLAPTGTIGLMMDCDTTGIEPDLALTKAKKLVGGGTMLIV
                     NQTIPRALAKLGYGADEVDKIVAYIDEHKTIIGAPAFKVEDLPVFACSMGDNAIHYRG
                     HVKMMAAVQPFISGAISKTVNMPEDVSIDDVGDLYLESWRLGLKAVAIYRDNCKVAQP
                     LATQKKASEGAGDAVSGEVERIVETIIVQEPVRQKLPRTRTAKTFSFRVADCHGYVTV
                     GEYEDGRPGEMFLQVAKQGSTLAGIMDAFAISVSHGLQYGVPLEAFVEMYTNMRFEPA
                     GMTDDADIRFATSLVDYIFRRLAVEYLPKEKREAMGILTVGERLQPTLPGVEEATTPS
                     ATALDLPLDDRPPSAALEQPKPVAPPAAAPAKQQREVVLCHVCGDMMQRAGSCYACPS
                     CGSTSGCS"
     gene            2327..>2853
                     /locus_tag="VFX21_04635"
     CDS             2327..>2853
                     /locus_tag="VFX21_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095272.1"
                     /translation="MVASVERLPSGEIVLSRYSQVANALRDPRFGKPPLPQVPVRALR
                     ALTEQFLLVDPPAHTRLRRVVAPTWTPAELAKMRPAVESIATTLLATRPDDFDLVRDF
                     AYPLPLTLIGDLLGVPAADRSQIATWTRTLTESIDVTPPRGLRDAGRVVRDVAARRWR
                     PMAAANAGIKVFKYA"
BASE COUNT          479 a          956 c         1005 g          413 t
ORIGIN      
        1 tgtcgaacat ccgctcgtcg tacgagcacc tcgcgggcgg cggcaccgcg agtggccccg
       61 tgtcgttcat gcgcggcgcc gacgcgtcgg ccgggacgat caagtcgggc ggcaagacgc
      121 gccgtgccgc gaagatggtc atcctcaacg ccgatcaccc cgacatcgaa gagttcgtgt
      181 ggtgcaaggc ggtcgaggag cgcaaggcgc gcgcgctggc cgaggccggc tttgacatgg
      241 acctcgacgg caaggacagc cactcgatcc agtaccagaa cgcgaacaac tcggtgcgcg
      301 tcaccgacga gttcatgcag gccgtcgtcg acgaccgcga ctgggcgctg cgcgcggtca
      361 cgaccggcga gccgctgaag acgctgcagg cgcgcgacct gtggcgccag atcgcggagt
      421 cggcgtggga gtgcgcggac ccgggcatgc agttcgacac gacgatcaac cgctggcaca
      481 ccgcggcggt gaccgggcgc atcaacgcct cgaacccgtg ctccgagtac atgcacctcg
      541 acaactcggc gtgcaacctc gccagcatca acctgttgaa gtacctcggc gacgacggcg
      601 cattcgacgt cgacggcttc aagcacacgg tcgacgtcgt gttcaccgcg caggagatcc
      661 tcgtcggcaa cgccgactac ccgacggagc cgatcgcgga cacgagccgc aagttccgcc
      721 agctcggtct cgggtacgcg aacctcggcg cgctgttgat ggcgcagggc atgccctacg
      781 actccgacgc cggccgggcg tgggcggccg cgatcaccgc gctgatgacg ggtcaggcgt
      841 atgcgacctc ggcgcgcacc gcggcgcgga tgggtccgtt cgcgggctac gccgacaacg
      901 cggagccgat gctgaacgtg ttgcgcatgc accgggcgga ggctgcccgc atcgacgaag
      961 acatcgtgcc gcccgagctg ctcggcgccg cgcagcagtc gtgggacgaa gcggtcgagc
     1021 tgggggagcg tcacggcgtc cgcaactcgc aggcctcggt cttggctccc accggcacca
     1081 tcggcctgat gatggactgc gacaccaccg gcatcgagcc cgacctcgca ctgacgaagg
     1141 cgaagaagct cgtcggcggc ggcacgatgc tcatcgtcaa ccagacgatc ccgcgtgcgc
     1201 tggccaagct cggatacggc gcggacgagg tcgacaagat cgtcgcctac atcgacgagc
     1261 acaagacgat catcggggcg cccgcgttca aggtcgaaga cctgccggtg ttcgcgtgct
     1321 cgatgggtga caacgcgatc cactaccggg gtcacgtgaa gatgatggcg gcggtccagc
     1381 cgttcatctc gggcgcgatc tccaagaccg tgaacatgcc cgaagacgtc tcgatcgacg
     1441 acgtcggcga cctctacctc gagtcgtggc ggttgggcct gaaggcggtc gcgatctacc
     1501 gcgacaactg caaggtcgcg cagccgttgg cgacgcagaa gaaggcgagc gagggcgcgg
     1561 gcgacgcggt gagcggtgaa gtcgagcgga tcgtggagac gatcatcgtc caggagccgg
     1621 tgcgccagaa gctgccgcgc acgcgcacgg cgaagacgtt ctcgttccgg gttgccgact
     1681 gccacgggta cgtgacggtg ggcgagtacg aagacggccg gcccggcgag atgttcctgc
     1741 aggtggcgaa gcagggttcg acgctcgccg gcatcatgga tgcgttcgcg atctcggtga
     1801 gccacggtct gcagtacggc gtgccgctcg aggcgttcgt ggagatgtac acgaacatgc
     1861 gcttcgaacc ggcgggcatg accgacgacg ccgacatccg gttcgcgacg agcttggtcg
     1921 actacatctt ccggcgcttg gcggtcgagt acctgcccaa ggagaagcgg gaggcgatgg
     1981 gcatcctcac ggtcggcgaa cggctgcagc cgacgttgcc cggtgtcgag gaagcgacga
     2041 cgccgagcgc gacggcactc gacctgccgc tcgacgaccg gccgccgtcg gccgcgttgg
     2101 agcagccgaa gccggtcgcg ccgcccgccg cggcaccggc gaagcaacag cgtgaagtcg
     2161 tgctctgcca tgtgtgcggc gacatgatgc agcgcgccgg cagttgctac gcctgcccca
     2221 gctgcggcag caccagcggc tgctcgtaac acctgtcaaa cctgcgccgc tcacccaccg
     2281 cggcggtggt tcagcggcgc aggtacgcgc gcgttaacgt gcgcgaatgg tcgcttcggt
     2341 ggaacggctg ccgagtgggg agatcgtgct ctcccggtac tcacaggtcg cgaacgcact
     2401 gcgcgatccg cgcttcggca agccgccgct tccgcaggtt ccggtacggg cgttgcgggc
     2461 gctcaccgag cagttcttgc tggtcgatcc gccggcgcac acgcgactgc ggcgcgtcgt
     2521 cgcccccacg tggacgccgg cggagctggc gaagatgcgg cccgcggtcg agtcgatcgc
     2581 gacgacattg cttgcgaccc gtcccgacga cttcgatctc gtgcgcgact tcgcgtaccc
     2641 gttgccgctg acgctgatcg gcgatctcct cggtgtgccc gcggccgacc ggtcgcagat
     2701 cgcgacgtgg acgcgcacgc tcaccgagtc gatcgacgtg acgccgccgc gcgggttgcg
     2761 cgacgcgggc cgcgtcgtgc gcgacgtcgc ggcgcgccgc tggcgcccga tggccgcggc
     2821 gaatgccggc atcaaggtct tcaagtacgc gca
//
LOCUS       DASUGA010000236         5981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1002542, whole genome shotgun sequence.
ACCESSION   DASUGA010000236 DASUGA010000000
VERSION     DASUGA010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002542"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1554)
                     /locus_tag="VFX21_04640"
     CDS             complement(<1..1554)
                     /locus_tag="VFX21_04640"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: protein motif:HMM:NF008559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HEX5095273.1"
                     /translation="MVHPLDMLSGDEVTKAAEILRASGKMPDGSLFAHLVLHEPAKED
                     LARWKTGDPVDREVRALIVPGPALHLVEAVVSVTAGEIREWREVHGMRPALLMTDAIN
                     AIFTTKEHPDYIAALAKRGITDLDNVQIDPWPAGVFGYAAEDERRIARCISFLRADDT
                     DNGYARPIEGVIVHFDMGRNEVIEVIDHGVTPLPPNRASYYAEDQPKLRTDLKPIHIT
                     QPDGPSFTVDGNFVEWQKWQFRISFDPYEGLVLHQVAYDDDGRVRPILHRASISEMVV
                     PYGDPGPVHGWKNAFDAGEWGLGRMTQPLTLGCDCLGEIRYFDATLATETGDPWVIEN
                     AICMHEEDYGIQWKHVDLFGGRGEVRRSRRLVVSFISTVGNYEYGFFWYLYLDGNIQL
                     EVKLTGIVSPMAITPGTQPEYANVVAEGVAAPHHQHLFNARLDFDVDGPVNEIYEVEA
                     ERVPAGPDNPWMNAFRPRATRLERESEAQRDMNAATSRVWKVVNPEVRNGLGQPVAYK
                     LVPTMSTPTM"
     gene            complement(1577..2305)
                     /locus_tag="VFX21_04645"
     CDS             complement(1577..2305)
                     /locus_tag="VFX21_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEX5095274.1"
                     /translation="MEPVSLEGVERILLVLAHPDDVDFGFAGGVATWTDAGIAVTYCI
                     VTDGDAGGAETGINRAEMAALRRDEQRAAAAVVGVDDVRFLAYPDGRVEASLDLRRDI
                     SRVIRDVRPQRVVCQSPERNWDRIYASHPDHLAAGEAAVAAVYPDARNRWAHPELADE
                     GFEPWSVDEMWVGAGAPGIPTHYTDITDAIDRKAEALCSHKSQIADPEGVGEMIRLWA
                     SGMAKLAGLPEGRYAEAVRVVNTR"
     gene            2325..2882
                     /locus_tag="VFX21_04650"
     CDS             2325..2882
                     /locus_tag="VFX21_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEX5095275.1"
                     /translation="MSFESRELVGLLADDNRRRVFAALVLGSSTLADVRRATGLDARD
                     ASRALQRFVDAGLVLADPDGTHHLLAEAFAMAARQDAARAPRSTEHADEPEDVARVLR
                     AFVRDGRLTSIPAQRGKRLVILDWLAQRFEPGRHYSESMVNLVLGQVHADTAALRRYL
                     VDEGFLDREHGEYWRSGGTVDGDGE"
     gene            2893..3699
                     /locus_tag="VFX21_04655"
     CDS             2893..3699
                     /locus_tag="VFX21_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5095276.1"
                     /translation="MSLVLVDKNVRPHVSVCTLNKPERLNSMSFPLVQALYGALDDIA
                     QDNDCWVVVLTGAGRGFCSGLDLHDRGVPPNSHDLGFSRLAMKSMSYMSDVVPAMRNI
                     PQPIIAAINGPAYGGGMCLTLGADVRYASDTAVFCSAGIINGLTSSELGATYLLPRAV
                     GTSNANELLLTGRKIDAAEALRIGLVSKVLPDGDVLNYALETAEEMTTNLSPFGFMMT
                     KQSSWSNIEISSLAAAIELENRNQLLAGYTGNLDEAIAAFKEKRKPSYKE"
     gene            complement(3700..4596)
                     /locus_tag="VFX21_04660"
     CDS             complement(3700..4596)
                     /locus_tag="VFX21_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5095277.1"
                     /translation="MMTDRYLVTGATGQVGFPVALGLARGGADVVAVARFKDPAKREQ
                     LEGAGVTCAEADFAKGALDAVPTGVDYVCNFAVVKSNKWDVDIAGNAEAVGMLMSHCR
                     GARAFLHCSSTGVYEAADGTPQRETDPLGDNHRVMMPTYSISKIAAEAVARTNARVFD
                     LPTTIARLNVPYGDNGGWPAFHLALMHAGRAVPVHPNGPSRYNPIHEDDIIALIPKML
                     EIATVPATIVNWGGDEETSIEEWCEYLAELTGCTATFDRDTPTIGGIPTDNTKRRELV
                     GPSTVGWKDGMRRMAIAQGYPL"
     gene            complement(4600..5298)
                     /locus_tag="VFX21_04665"
     CDS             complement(4600..5298)
                     /locus_tag="VFX21_04665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEX5095278.1"
                     /translation="MTDDRDLEGLSPYDLMDAEVERLDRYFDALSGAAWDAPSRCDGW
                     SVRHVLAHLTSSEDYNRACLDGNVQALMQQWGSQGATDLDGVNELGVRAWDGTDPKEI
                     LAVWRERIGTNRRDFRARDGNVVDSSVGPYPARWQAFHLAFELAVHADDVFVPVDPSE
                     EPARTAWQARFGRFAISELKPDATIERGAGSTRVIGPGVDIELADPEFVAVVSGRAGD
                     SGLDAAVLDYVRAT"
     gene            5390..5533
                     /locus_tag="VFX21_04670"
     CDS             5390..5533
                     /locus_tag="VFX21_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006545243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPGG-motif small membrane protein"
                     /protein_id="HEX5095279.1"
                     /translation="MLFLLWLLAVILVVSGIVALVRGAIAYGLVLIIVGFLVGPGGAS
                     IFT"
     gene            complement(5530..>5981)
                     /locus_tag="VFX21_04675"
     CDS             complement(5530..>5981)
                     /locus_tag="VFX21_04675"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5095280.1"
                     /translation="RSTEMAMSSDVRTYAAAVRDFEAAAQNVAAQHEVRLVKLIGDAA
                     MLASRRAPDVVAAAASLVANIAADNRFEAARGGVAFGRVVARDGDYYGPAVNLAARLS
                     GRAESGAVLVDEAVARSVPDIVIDAGTQQLKGFSDAQRVFALRTAES"
BASE COUNT          915 a         2055 c         1992 g         1019 t
ORIGIN      
        1 catcgtcggc gtgctcatcg tcggcacgag cttgtaggcc accggctggc ccaggccgtt
       61 gcgcacctcg gggttgacca ccttccagac gcgtgaggtc gcggcgttca tgtcgcgctg
      121 cgcctccgac tcgcgctcca gtcgcgtggc gcgcgggcgg aacgcgttca tccacggatt
      181 gtcgggcccg gccggcacgc gctcggcctc gacctcgtag atctcgttca ccggaccgtc
      241 gacgtcgaag tcgagacgcg cgttgaacag gtgttggtga tgcggcgccg cgacaccttc
      301 cgcgacgacg ttcgcatact cgggttgcgt accgggcgtg atcgccatcg gcgagacgat
      361 gccggtgagc ttcacctcga gctggatgtt gccgtcgagg tagaggtacc agaagaagcc
      421 gtactcgtag ttgccgacgg tcgagatgaa gctgaccacg agccggcggc tgcggcgcac
      481 ttcgccgcga ccgccgaaga ggtcgacgtg cttccactgg atgccgtagt cctcttcgtg
      541 catgcagatc gcgttctcga tcacccacgg gtcgccggtc tcggtcgcga gcgtggcgtc
      601 gaagtaacgg atctcgccga ggcagtcgca gcccagagtg aggggttggg tcatgcgccc
      661 gaggccccac tcccctgcgt cgaacgcgtt cttccacccg tgcaccgggc ccggatcgcc
      721 gtagggcacg accatctcgc tgatcgacgc ccggtgcagg atcgggcgca cacggccgtc
      781 gtcgtcgtag gcgacctggt gcagcaccag cccttcgtac gggtcgaacg agatgcggaa
      841 ctgccacttc tgccactcca cgaagttgcc gtcgacggtg aagctcggcc cgtcgggctg
      901 tgtgatgtgg atcggcttca ggtcggtgcg cagcttcggc tggtcttcgg cgtaatagct
      961 ggcccggttg ggcggcagcg gcgtcacacc gtggtcgatg acctcgatca cctcgttgcg
     1021 gcccatgtcg aagtggacga tgacgccctc gatcgggcgc gcgtagccgt tgtcggtgtc
     1081 gtccgcgcgc aggaacgaga tgcagcgcgc gatgcgccgc tcgtcttccg cggcgtagcc
     1141 gaacacaccg gccggccacg ggtcgatctg cacgttgtcg aggtcggtga tgccgcgctt
     1201 cgccagtgcg gcgatgtagt cggggtgttc cttcgtcgtg aagatcgcgt tgatggcgtc
     1261 cgtcatcagc agcgcgggcc gcatgccgtg cacttcgcgc cactcgcgga tctcgccggc
     1321 ggtcaccgag acgacagctt cgacgaggtg cagcgccggt cccggcacga tcagcgcgcg
     1381 cacctcgcgg tcgacgggat caccggtctt ccagcgcgcg aggtcttcct tggccggctc
     1441 gtgcaacacg aggtgggcga acagcgaacc gtcgggcatc ttccccgacg cgcgcaggat
     1501 ctcggcagcc ttcgtcacct cgtcgccgct gagcatgtcg agcggatgca ccatcactgt
     1561 cctcctgatg cgtgtcctaa cgcgtgttca cgacgcgcac tgcttcggca tagcggcctt
     1621 cgggcagacc cgcgagcttc gccatcccac tcgcccacag gcggatcatc tcaccgacac
     1681 cctcggggtc ggcgatctga ctcttgtgcg agcacagcgc ctccgccttg cgatcgatcg
     1741 cgtcggtgat atcggtgtag tgcgtcggga tcccgggcgc gccggcaccg acccacatct
     1801 cgtcgaccga ccacggttcg aaaccttcgt ccgcgagctc cggatgcgcc cagcggttgc
     1861 gggcgtcggg atagacggcc gcaaccgccg cttcgccggc cgcgagatga tcggggtggc
     1921 tcgcgtagat gcgatcccag ttgcgctcgg gcgactggca cacgacgcgc tgcggtcgca
     1981 cgtcgcggat cacgcgcgag atgtcgcgac gcaggtcgag cgatgcttcg acgcgaccat
     2041 cggggtaggc caggaaacgg acatcgtcga caccgacgac tgcggccgcc gcccgctgtt
     2101 cgtcgcgtcg cagcgcggcc atctcggccc ggttgatgcc ggtctccgca ccaccggcgt
     2161 cgccgtcggt gacgatgcag taggtcacgg caatgccggc gtcggtccac gtcgcgaccc
     2221 cgcccgcgaa cccgaagtcg acgtcgtcgg ggtgcgccag caccagcagg atgcgctcga
     2281 caccttcgag tgagaccggt tccacgggtg cgaagctatc cgctgtgagc ttcgagagcc
     2341 gtgagctggt cggactgctg gccgacgaca accggcggcg cgtgttcgcg gcactcgtgc
     2401 tcggttcgtc caccttggcc gacgtccggc gcgccaccgg actcgatgca cgagacgcgt
     2461 cgcgcgcgtt gcagcgcttc gtcgacgccg gtctcgtgct cgccgacccc gacggcaccc
     2521 atcatctcct cgctgaggcg ttcgcgatgg ccgcccgtca agacgcggcc cgcgcaccgc
     2581 gcagtaccga acacgcggac gaaccggaag acgtggcgcg agtgctacgt gcgttcgtgc
     2641 gcgacggccg gctgacgtcg atccccgcgc aacgcggcaa gcggctggtg atcctcgact
     2701 ggctcgcgca acgcttcgag cccggccgtc attactccga gtcgatggtg aacctcgtcc
     2761 tcggccaggt gcatgccgat acggccgcgc tgcgccgcta cctcgtcgac gaggggttcc
     2821 tcgaccgtga gcacggcgag tactggcgct cgggcgggac cgtcgacggc gacggcgagt
     2881 agcttcccgc gcatgagttt ggtcctggtc gacaagaacg ttcgtcccca cgtcagcgtc
     2941 tgtacgttga acaagcccga gcgcctgaac tcgatgtcgt tcccgctggt gcaggcgctc
     3001 tacggagcgc tcgacgacat cgcgcaggac aacgactgct gggtcgtcgt gctcaccggc
     3061 gcgggccgcg gcttctgctc ggggctcgac ctgcacgacc gcggcgtgcc tcccaacagt
     3121 cacgatctcg gtttctcgcg cctggccatg aagtcgatgt cgtacatgag cgacgtcgtg
     3181 cccgcgatgc gcaacatccc gcagccgatc atcgccgcga tcaacgggcc cgcgtacggc
     3241 ggcggcatgt gcctcaccct cggtgccgac gtgcggtacg cgtccgacac cgccgtgttc
     3301 tgctccgccg gcatcatcaa cggcctgacg tcgtccgagt tgggtgcgac gtacctcttg
     3361 ccccgcgcgg tgggcacgtc gaatgcgaac gagctcctgc tcacggggcg caagatcgac
     3421 gcggcggaag cgctgcgcat cgggctcgtc tcgaaggtgt tgcccgacgg tgacgtgctg
     3481 aactatgcgc tcgagaccgc cgaggagatg acgaccaacc tgtcaccgtt cgggttcatg
     3541 atgacgaagc agtcgtcgtg gtcgaacatc gagatctcgt cgctggcggc ggcgatcgag
     3601 ctggagaacc gcaatcagct gctcgcgggc tacaccggca acctcgacga ggcgatcgcc
     3661 gcgttcaagg agaagcgcaa gccgagctac aaggagtagc tacaaggggt aaccctgcgc
     3721 gatcgccatg cggcgcatgc cgtccttcca gccgacggtg ctcggcccga cgagctcccg
     3781 gcgcttggtg ttgtcggtgg gtatgccgcc gatcgtcggc gtgtcccggt cgaacgtcgc
     3841 ggtgcatccc gtgagctcgg cgaggtattc gcaccactcc tcgatgctcg tctcctcgtc
     3901 gccgccccag ttcacgatcg tcgcgggcac cgtcgcgatc tccagcatct tggggatcag
     3961 cgcgatgatg tcgtcctcgt ggatcgggtt gtagcgactc ggcccgttcg ggtgcacggg
     4021 caccgcgcgg cccgcgtgca tgagggccag gtggaacgca ggccaaccac cgttgtcgcc
     4081 gtagggcacg ttgagccgcg cgatcgtcgt cgggagatcg aagacgcgcg cgttcgtgcg
     4141 ggcgaccgct tcggcggcga tcttcgagat gctgtaggtc ggcatcatca cgcggtggtt
     4201 gtcgcccagc ggatccgtct cgcgctgtgg tgtgccgtca gccgcttcgt acacgccggt
     4261 cgacgagcaa tgcaggaagg cacgggcacc acggcagtgt gacatgagca tgccgaccgc
     4321 ctcggcgttg ccggcgatgt cgacgtccca cttgttcgac ttcacgaccg cgaagttgca
     4381 cacgtagtcg acgccggtcg gcaccgcgtc gagtgcgccc ttggcgaagt cggcctcggc
     4441 gcacgtcaca cccgcgcctt cgagttgttc gcgcttggcc ggatccttga accgcgcaac
     4501 cgcgacgaca tcggcgccgc cacgcgcgag acccaaggca accgggaacc cgacctggcc
     4561 ggtggcaccc gtgacgagat agcgatcagt catcactcgt catgttgccc gcacgtagtc
     4621 aagtactgcc gcatcgagcc ccgagtcacc ggcccggccc gagacgacgg cgacgaactc
     4681 cggatcggcc agttcgatgt cgacgcccgg cccgatcact cgggtcgagc ccgcaccccg
     4741 ttcgatcgtc gcgtcgggct tcagctcgct gatcgcgaag cgtccgaacc gcgcctgcca
     4801 cgccgtgcgc gcaggctcct ccgacgggtc gacgggcacg aagacgtcgt cggcatgcac
     4861 cgcgagctcg aacgcgaggt ggaacgcctg ccagcgcgcc ggatacgggc cgacggagct
     4921 gtcgacgaca ttcccgtcgc gagcgcggaa gtcgcgacga ttggtgccaa tgcgctcgcg
     4981 ccacaccgcc aggatctcct tcgggtccgt gccatcccag gcgcgtacac cgagctcgtt
     5041 gacgccgtcg agatctgtcg caccttgcga tccccactgc tgcatcagcg cctggacgtt
     5101 cccgtcgaga cacgcgcggt tgtagtcctc gctggacgtc aggtgcgcga gcacgtggcg
     5161 caccgaccaa ccatcgcagc ggctcggcgc gtcccacgcc gcccccgaca acgcatcgaa
     5221 gtagcggtcg agccgctcga cctcggcgtc catcaggtcg tacggactga gtccttcgag
     5281 atcacgatcg tcggtcacgg ccgaacggta gcgcgggcgt tgtcagtacc taaacgtgta
     5341 tctgcagaaa ttctcgggta gcgcgaggcc agcaaaggag gcattcccca tgctgttctt
     5401 actgtggctt ctcgccgtga tcctcgtagt gtcgggcatc gttgcgctcg tgcgcggggc
     5461 gatcgcgtac gggttggtgc tcatcatcgt cgggttcctc gtcggccccg gcggcgcaag
     5521 catcttcacc tagctctccg cagtgcgcag cgcgaacacg cgctgcgcgt cactgaagcc
     5581 cttcaactgt tgcgtgcccg catcgatgac gatgtcgggc acgcttcgcg cgacggcctc
     5641 gtcgacgagc accgctccgg actctgcacg tcccgacagg cgcgcggcca gattcactgc
     5701 gggtccgtag tagtcgccgt cacgcgcaac gacccggccg aacgcgacgc cgccgcgcgc
     5761 ggcctcgaat cggttgtcgg cggcgatgtt cgcgacaagt gacgccgccg cggcgacgac
     5821 atcgggagcg cggcgagacg caagcatcgc ggcgtcgccg atgagcttca ccaggcggac
     5881 ctcgtgttgt gccgcgacgt tctgggcggc ggcctcgaag tcgcgcaccg ccgccgcgta
     5941 ggtacgcacg tcggacgaca tcgccatctc ggtcgagcgc a
//
LOCUS       DASUGA010000237         2239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_584410, whole genome shotgun sequence.
ACCESSION   DASUGA010000237 DASUGA010000000
VERSION     DASUGA010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584410"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VFX21_04680"
     CDS             complement(<1..289)
                     /locus_tag="VFX21_04680"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX5095281.1"
                     /translation="MALLDDSEVERVLVVVAHPDDIDFGVAGSVAMWTDAGIEVHYCL
                     VTSGDAGGHDVSVSRSEMVEIRQREQTEAAKVVGVTDLHWLGFPDGRVEPSL"
     gene            330..731
                     /locus_tag="VFX21_04685"
     CDS             330..731
                     /locus_tag="VFX21_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020659064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX5095282.1"
                     /translation="MLPVTWDTIRVFLHVAAATIWVGGQFTLAGLVPGLRGISPDAPR
                     AVARRFNLIAWPAYFVLIATGIWNVIAIDPTFDTPYGRTLMVKVVVAILSGLAAFLHS
                     IARSKPGLAIWGAVSSLSAITALFLGVMLAT"
     gene            complement(728..1804)
                     /locus_tag="VFX21_04690"
     CDS             complement(728..1804)
                     /locus_tag="VFX21_04690"
                     /EC_number="1.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018506 - maleylacetate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetate reductase"
                     /protein_id="HEX5095283.1"
                     /translation="MNAFVYESQPGRVVFGLGAQARIAEEVDRLGAERVLLLSSPSAT
                     AAADALHAALGPVALRWTEVAEHVPVGLAERARAAAWDAHVDVIVTIGGGSAIGLAKA
                     VALELGAPIVAVPTTYAGSEMTPMYGLTGDHKVTGRDSRVLPRVVVYDPDLTLGLPID
                     TSIASACNALAHCIEGCYGPGANPVTTLSAIEGIRVLSANVPLLARDARDVDVRSELL
                     YGAYLAGTVIASVGVGVHHRTCHVLGGSYGLAHAASNAVILPHAIAYNAPAVPDLVAR
                     WRDAMGTSDPAGFAFDLVADAGLPTSLASLGLPEAALDDAAARIVEETPNNPRPVDVS
                     SVRAMLDGAWRGVRPTSASPTAAR"
     gene            1859..>2239
                     /locus_tag="VFX21_04695"
     CDS             1859..>2239
                     /locus_tag="VFX21_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5095284.1"
                     /translation="MAESELDYDALDAERERIRSEYMRESPGPSTARGVHHVALLSSD
                     VEQTVRFYQDLLEFPLTDMFENRDYRGSTHFFFDIGNGNALAFFDFPGLGLGPYAEVL
                     GGLQHLAISVAPDTWSRLKDKLDAA"
BASE COUNT          349 a          829 c          726 g          335 t
ORIGIN      
        1 cgagtgacgg ctcgacccga ccgtccggaa acccgagcca gtggagatcg gtcaccccca
       61 cgaccttcgc cgcctcggtc tgctcgcgct ggcggatctc caccatctcg ctccggctca
      121 ccgagacatc gtgcccaccg gcgtcgccac tcgtgacgag gcagtagtgc acctcgatgc
      181 ccgcgtcggt ccacatcgcg acgctgccgg cgacgccgaa gtcgatgtca tccggatgcg
      241 cgacgaccac caggacgcgt tcgacctcgg aatcatcgag caatgccata cgaccaaggg
      301 taacgacggc cgctagcgtt cggattccga tgcttccggt gacctgggac accattcgcg
      361 tgttcctcca cgtcgccgcc gcaaccatct gggtcggcgg tcagttcaca ctcgccgggc
      421 tcgtaccggg actgcgcggc atctcccccg acgcgccgcg cgccgtcgcg cgacggttca
      481 acctcatcgc gtggcccgcg tacttcgtgc tcatcgcgac cgggatctgg aacgtcatcg
      541 cgatcgaccc gacgttcgac acgccctacg gccgcacgct catggtgaaa gtcgtcgtgg
      601 ccatcctctc gggcctggcc gcgttcctgc actcgatcgc gcggtcgaag ccgggcctgg
      661 cgatctgggg tgccgtgagc tcactctcgg cgatcaccgc gctgttcctc ggcgtcatgc
      721 tcgcgaccta gcgcgctgcc gtcgggctgg cgctcgtcgg gcggacgccg cgccaggcgc
      781 cgtcgagcat ggcgcgcacc gacgacacgt cgaccggccg cgggttgttc ggcgtctcct
      841 ccacgatccg cgccgcggcg tcgtcgagcg cagcttccgg gagcccgagc gaagccaagc
      901 tcgtgggcag gccggcgtcg gcgacgaggt cgaacgcgaa accggccggg tcggacgttc
      961 ccatcgcgtc gcgccagcgc gccacgaggt ccggaaccgc cggcgcgttg tacgcgatgg
     1021 cgtgcggcag gatcaccgcg ttgctcgcgg catgcgcgag tccgtacgag ccgccgagga
     1081 cgtgacacgt gcgatggtgc acgcccactc cgacgctcgc gatgaccgtc cccgcgaggt
     1141 acgcaccgta gagcagctcg ctccgcacgt cgacgtcacg cgcatcccgc gcaaggagcg
     1201 gcacgttcgc actcagcacg cggatgcctt cgatcgccga cagcgtggtc acgggattgg
     1261 cgccgggccc gtagcagccc tcgatgcagt gcgcgagcgc gttgcacgcg ctcgcgatgc
     1321 tcgtgtcgat cggcaagccg agcgtgagat cggggtcgta caccacgacg cgcggcagca
     1381 cccgcgaatc gcgacccgtg accttgtggt cgccggtcag cccgtacatg ggcgtcatct
     1441 ccgagcccgc gtaggtggtc ggcaccgcga cgatgggcgc acccagctcg agcgccacgg
     1501 ccttggcgag cccgatcgcc gaacccccgc cgatcgtgac gatcacgtcg acgtgcgcat
     1561 cccacgccgc cgctcgggca cgttcggcga gccccacggg cacgtgctcc gccacctcgg
     1621 tccaacgcag cgcgaccgga cccagtgcgg cgtgcagcgc gtcggccgca gcagtggccg
     1681 aaggcgacga cagcaggagc acgcgctcgg cgccgaggcg atcgacttcc tcggcgatcc
     1741 gggcctgcgc gcccaagccg aacaccacac gtcccggctg ggactcgtac acgaacgcgt
     1801 tcactcctac gatcatcgca ctgatcagcc cgtggtagag gtgggagagg aggatgccat
     1861 ggccgagtcc gaactcgact acgacgcgct ggacgccgaa cgcgaacgca tccgttcgga
     1921 gtacatgcgc gagtccccgg gaccgtcgac cgcacgcggc gtgcaccacg tcgcgctctt
     1981 gagctccgat gtcgagcaga ccgtccgctt ctaccaggac ctgctcgagt tcccgctcac
     2041 cgacatgttc gagaaccgcg actaccgcgg gtcgacccac ttcttcttcg acatcggcaa
     2101 cggcaacgca ctcgcgttct tcgatttccc gggcctcggc ctgggcccgt acgccgaagt
     2161 gctcggcgga ctgcagcacc tcgcgatctc ggtggcgccg gacacgtggt cccggttgaa
     2221 ggacaagctc gacgccgcc
//
LOCUS       DASUGA010000238         2080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_359632, whole genome shotgun sequence.
ACCESSION   DASUGA010000238 DASUGA010000000
VERSION     DASUGA010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359632"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VFX21_04700"
     CDS             <1..576
                     /locus_tag="VFX21_04700"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX5095285.1"
                     /translation="GSPDTGEHVWILGAPPPSASEPWMTSGIVTSTNSLVAKDAGPIT
                     SGLLETDAASNATAAGGALVDAHGEVTGIVIGHVDDSPTTFAVPIQTAVAIAEELRDN
                     GKAEHGSLGLEGKDAPLPMVMSVVKDGPADKAGIRAGDVVSAVDGRAVDSIGAVTAII
                     RARHPGETVTISLRRGRDDLTMRVPLGAVNG"
     gene            614..1684
                     /locus_tag="VFX21_04705"
     CDS             614..1684
                     /locus_tag="VFX21_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HEX5095286.1"
                     /translation="MADDLDDLDREILNAVQWDFPLEPRPFAALGERLGLSEPEVRER
                     IAKVKDAGVLRQLSAIFDTRALGYSSALVAAKIDPDRIDDAAAVVSEHPGVSHNYKRN
                     HDYNLWYTIAVPPGDSLDEHIDVLHRDSGASVTRKLPTITLYKIGVKLDMTGKTAADA
                     KAEVLEHERPERKEVMPAPELSDFELAAIRVVQHDLPNIERPFAAYAEQIGALLGRDV
                     SEDDVLDALRSFKERKLMRRFAAVMNHRSAGFKANAMGVWAVPEELLGEIGPQMAGFA
                     AVSHCYRRPTYDDWPYSVFTMVHGRSARDCEATIDAIKTETGIDEYCLLWSIKEYKKV
                     RLEYYTSDWDSWAAKHLPATAR"
     gene            complement(1681..>2080)
                     /locus_tag="VFX21_04710"
     CDS             complement(1681..>2080)
                     /locus_tag="VFX21_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095287.1"
                     /translation="LELATVDDLDGAFNVAADGWLTADEATALLPGRRMPAIPSEVAT
                     RVLATTWSTGIGDAPPTVVPYLAHPWVVGNDRLKSAGWKPKHSNDEAILLATTGGEAS
                     LWPWIASAAAVTTGAAIGTWWLTRRRRRRR"
BASE COUNT          377 a          703 c          706 g          294 t
ORIGIN      
        1 ggcagtcccg acaccggcga gcacgtgtgg atactgggcg cgccgccgcc gagcgcgtcg
       61 gaaccgtgga tgacgagcgg catcgtcacc tcgaccaact cactggttgc gaaggacgca
      121 ggtccgatca cgagcgggct gctcgaaacc gacgccgcca gcaacgccac cgcggccggt
      181 ggcgcgctcg tcgacgcgca cggcgaggtg accggcatcg tcatcggtca cgtcgacgac
      241 agcccgacga cattcgcggt gccgatccaa acggcggtcg ccatcgccga ggaactccgc
      301 gacaacggca aggcggaaca cgggtcgctc ggcctcgaag gcaaggacgc gccgctgccg
      361 atggtgatga gtgtggtgaa ggacggaccg gccgacaaag cgggcatcag ggccggcgac
      421 gtcgtgagcg ccgtcgacgg acgagcggtc gactcgatcg gcgccgtgac ggccatcatt
      481 cgggcccgcc atcccggtga gacggtgaca atttcgctgc ggcggggccg cgacgatctc
      541 acgatgcggg tgccactcgg cgccgtcaac ggctgagttc gaacccggac cggccacgcg
      601 cgaccatggg agcgtggcag acgacctcga cgatctcgac cgcgaaattc tgaacgcggt
      661 ccagtgggac ttcccgctcg agccccggcc gtttgcggcg ctcggcgagc ggctgggcct
      721 ctccgaacca gaggtgcgcg aacgcatcgc caaggtgaaa gacgcgggcg ttttgcgcca
      781 gctgtccgcg atcttcgaca cgcgggcgct gggttactcg tccgcgctgg tcgccgcgaa
      841 gatcgacccg gaccgtatcg acgacgcggc cgcggtcgtc agcgagcacc cgggcgtcag
      901 tcacaactac aagcgcaacc acgattacaa cctctggtac acgatcgcag ttccgcccgg
      961 cgactcactc gacgagcaca tcgacgtcct gcaccgcgat tcgggtgctt cggtgacgcg
     1021 aaagctgccg acgatcacgc tgtacaagat cggcgtgaaa ctcgacatga ccggtaagac
     1081 cgccgccgac gcgaaggccg aagtactcga acacgaacgg ccggaacgca aagaggtcat
     1141 gccggcgccc gaactctccg acttcgaact ggccgcgatt cgggtcgtgc aacacgactt
     1201 gccgaacatc gagcgaccgt ttgccgcgta cgcggagcaa atcggcgcgc tcctcggacg
     1261 cgacgtcagc gaggacgacg tgctcgacgc gctgcggtcg ttcaaggagc gcaagctcat
     1321 gcgccggttt gcggcggtca tgaaccaccg cagcgccggc ttcaaggcga acgcgatggg
     1381 cgtgtgggca gtccccgaag aactgttggg cgagatcggt ccgcagatgg ccgggttcgc
     1441 cgccgtctca cactgttacc ggcggccgac ctacgacgac tggccctaca gcgtgttcac
     1501 gatggtgcac gggcgcagcg cgcgtgattg cgaagcgacg atcgacgcca tcaagaccga
     1561 gacgggcatc gacgagtact gcctgctctg gtcgatcaag gaatacaaga aggtgcggct
     1621 cgagtactac acctccgact gggacagctg ggccgccaag cacctgccgg caaccgcgcg
     1681 ctagcggcgg cgtcgccgcc ggcgggtcag ccaccacgtt ccgatcgcgg caccggtcgt
     1741 gaccgcggcc gcgctcgcga tccacggcca caacgacgct tcgccgccgg tcgtggcgag
     1801 caagatcgct tcgtcgttgc tgtgcttcgg tttccagccg gccgatttca agcgatcgtt
     1861 cccgacgacc cacgggtgcg cgagatacgg cacgaccgtc gggggcgcgt cgccgatacc
     1921 ggtcgaccac gttgtggcga gcacgcgggt cgcgacttcg ctcgggatcg cgggcatgcg
     1981 ccggccgggc aacaacgcgg tcgcctcgtc cgcggtcagc cagccgtcgg ccgcgacatt
     2041 gaacgcgccg tcgaggtcgt cgaccgtcgc gagttcgagc
//
LOCUS       DASUGA010000239         2460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_915180, whole genome shotgun sequence.
ACCESSION   DASUGA010000239 DASUGA010000000
VERSION     DASUGA010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915180"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VFX21_04715"
     CDS             complement(<1..531)
                     /locus_tag="VFX21_04715"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX5095288.1"
                     /translation="MASSIRLLVSDIDGTLITPDKTLTDRTVDAVRRLHAAGILFAIT
                     SSRPPPGLAMYATPLQVTTPLGAYNGGAYCDPTTLQVLDHRAIPDAAVAPLVETIEAA
                     GLQAWLYRGGAWLVRDVDAWHVQHEAEVVQFTPTEVADFGDLEGADAADVVKVVGVGQ
                     DHDAVKRVESEIRAQLG"
     gene            576..1385
                     /locus_tag="VFX21_04720"
     CDS             576..1385
                     /locus_tag="VFX21_04720"
                     /inference="COORDINATES: protein motif:HMM:NF019633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HEX5095289.1"
                     /translation="MTTPRLRREPPRFRSVSLLRREPITPRLVRCTFGGDDLTGFPVP
                     DPAASVRVLFPEPGTTGLVMPEWNGNEFLLPGSGGGTRLRPYIRTFTPRVTRTEGPNA
                     PELDVDVVLHGAGVASSWVETAELGAPAAVSGPGRGYAVSADAPPHLLAGDESAIPAM
                     SQLLEVLPATVDVIVHVEIGDAAAHHPLPPHPRADVHWHELAASTPPGDSLVAAIEAA
                     TIGDDTRVWAAGEAAAMQRIRRHLFDTRGIPRPRTTIRGYWKFGRAGTVDD"
     gene            complement(1401..>2460)
                     /locus_tag="VFX21_04725"
     CDS             complement(1401..>2460)
                     /locus_tag="VFX21_04725"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003607264.1"
                     /note="catalyzes the formation of D-glucono-1,5-lactone
                     6-phosphate from D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX5095290.1"
                     /translation="TAVQLLDKAELADGSRIIMEKPFGTDLASAMGLNDQLHLTFNED
                     QIFRIDHFLGKEAALNILAFRFANGLFEPIWNREHIAHVQIDVPEALEIGHRIKFYEE
                     TGAYRDMVVNHLFQVLAFMAMEPPTSLAPRPIREEKDKVFRCMKPIEPSDVVRGQYDG
                     YLDEAGVRPNSETETFVALKCEIDNWRWADVPFYLRTGKRMAESARIISIAFREPPKS
                     MFPTGSGVGAHGPDHLTFDLADVSKLSLSFYGKRPGPGMKLDKQSLQFSLHETGKAGD
                     VLEAYERLIYDAMLGDHTLFSSAEGIESLWDRSSPLLENPPPVRTYRPGSWGPNAIHQ
                     LIAPNAWRLPFERKWRDS"
BASE COUNT          354 a          871 c          840 g          395 t
ORIGIN      
        1 tcccagctgc gcccggatct ccgattccac gcgcttcacg gcgtcgtggt cctggccgac
       61 gccaaccacc ttcaccacgt ccgcggcgtc ggcgccctcg agatcgccga agtcggccac
      121 ctccgtcggc gtgaactgca cgacttcggc ttcgtgctgc acgtgccacg cgtccacgtc
      181 gcgcacgagc cacgcgccgc cgcgatacag ccacgcctgc aagccggccg cttcgatggt
      241 ctcgacgagt ggcgccaccg cggcgtcggg gatcgcgcgg tgatcgagca cctgcaacgt
      301 cgtcgggtcg cagtacgcgc cgccgttgta cgcaccgagc ggtgtggtca cctgcaacgg
      361 cgtcgcgtac atcgccagcc ccggcggcgg gcggctgctc gtgatcgcga acaggatccc
      421 cgcggcgtgg aggcgccgca ccgcgtcgac ggtccggtcg gtcagcgtct tgtcgggcgt
      481 gatgagcgtg ccgtcgatgt cggacacgag cagccggatc gacgatgcca cggcctcgag
      541 ccttgcatac acgcacgtgg cacgatgctg aaccgatgac gacaccgcgc ctgcgccgcg
      601 agccaccccg ttttcgcagc gtgtcgttgc tgcgacgcga gccaatcacg ccccgcctcg
      661 tgcgctgcac cttcggcggc gacgacctca ccggcttccc ggtgccggat cccgcggcca
      721 gcgtgcgcgt gctgttcccc gagcccggta cgaccgggct ggtgatgccg gaatggaacg
      781 gcaacgagtt cctgctcccc ggctcgggcg gtggcacacg gctccggccg tacatccgca
      841 cgttcacgcc gcgtgtgacg cgcaccgagg gccccaacgc tcccgagctc gacgtcgacg
      901 tcgtgctcca cggcgccggt gtcgcgtcct cgtgggttga gaccgccgag ctcggcgcgc
      961 cggctgcggt gtccggtcct ggccgcggct acgcggtcag cgcggacgcg ccgccccacc
     1021 tcctcgccgg agacgagagc gcgatcccgg cgatgagcca gctcctcgaa gtgctccccg
     1081 cgaccgtcga cgtgatcgtg cacgtcgaga tcggcgacgc cgcggcgcac cacccgctgc
     1141 cgccgcaccc gcgcgccgac gtgcactggc acgagcttgc cgcgagcacg ccacccggcg
     1201 actcgctcgt cgccgcgatc gaagccgcga ccatcggcga cgacacgcgc gtgtgggccg
     1261 ccggcgaagc cgcggcgatg cagcgcattc gtcgacatct gttcgacacg cgcggcatcc
     1321 cgcggccgcg cacgacgatc cgcggctact ggaagttcgg gcgcgccggc accgtcgacg
     1381 actgacgcgt acacgcgcgg ctacgagtcg cgccacttcc gttcgaacgg cagccgccac
     1441 gcgttcggcg cgatcagctg gtggatcgcg ttcgggcccc acgagcccgg tcggtacgtg
     1501 cgcacgggcg gtgggttctc gagcagcggt gacgaccgat cccacaggct ctcgatcccc
     1561 tccgcgctcg agaacagtgt gtggtcaccg agcatggcgt cgtagatcaa gcgctcgtac
     1621 gcctcgagca cgtcgccggc cttgccggtc tcgtgcagcg agaactgcag gctctgcttg
     1681 tcgagcttca tgccgggacc cggccgcttg ccgtagaacg acagcgacag cttcgacacg
     1741 tcggcgaggt cgaaggtcag gtggtcgggg ccgtgcgcgc cgacacccga tccggtcggg
     1801 aacatgctct tgggcggctc acggaacgca atggagatga tgcgtgcgct ctccgccatc
     1861 cgcttgccgg ttcggaggta gaagggcacg tcggcccagc gccagttgtc gatctcgcac
     1921 ttgagcgcga cgaacgtctc ggtctcggag ttggggcgca cgccggcctc gtcgaggtag
     1981 ccgtcgtact ggccgcgcac gacgtcggac ggctcgatcg gcttcatgca ccggaagacc
     2041 ttgtccttct cctcgcggat cggacgcggt gcgagcgacg tcggcggctc catcgccatg
     2101 aacgcgagca cctggaacag gtggttcacg accatgtcgc ggtacgcgcc ggtctcttcg
     2161 tagaacttga tccggtgtcc gatctcgagc gcttcgggga cgtcgatctg cacgtgcgcg
     2221 atgtgctcgc ggttccagat cggctcgaag agcccgttcg cgaagcggaa ggcgaggatg
     2281 ttgagcgcgg cttccttgcc caggaagtgg tcgatgcgga agatctggtc ctcgttgaac
     2341 gtgaggtgga gctggtcgtt cagtcccatc gcgctcgcga ggtcggtgcc gaacggcttc
     2401 tccatgatga tgcgagagcc gtcggcgagc tcggccttgt cgagcagctg gactgcggtg
//
LOCUS       DASUGA010000240         4732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1695904, whole genome shotgun sequence.
ACCESSION   DASUGA010000240 DASUGA010000000
VERSION     DASUGA010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695904"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VFX21_04730"
     CDS             complement(<1..613)
                     /locus_tag="VFX21_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX5095291.1"
                     /translation="MPDRTSESWDDVVEDLAQRRAASRAMGGDERLAKHRAAGKLDVR
                     ARIAHLLDPGSFLELGTLVGGEDSPADALVMGSGRIDGRPVMVAAEDFTVKAGTISGA
                     ANAKRYRVADIAVRDRVPCIMVLEGAGYRADGKPHIAPTPTDMLAQARCSGRVPLVTA
                     VLGASAGHGALVAPISDFSVMTTRAAIFTAGPPVVYESMGEEIT"
     gene            complement(619..1422)
                     /locus_tag="VFX21_04735"
     CDS             complement(619..1422)
                     /locus_tag="VFX21_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018637162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5095292.1"
                     /translation="MSHPNIRVERDDLVATVTLDRPEAKNACTGDMWVEIGAAFRDLS
                     HAGVRAIVLTGANGDFCTGADLGGGGSGSGGGSGGPQAIPPMVDSMRVLADVVLAVHD
                     CPIPVVAKVDGLCVGAGLGLALAADITYCSERARFSAIFARRGLSLDFGSSWLLRQRI
                     GAHKAKELAFTAKMVSADQASELGLVNAVFPAAGLDESVREIVATIAAGPPIALASTK
                     REIDNASAMSLAQALEIEALAQSVNVQTEDLREALMAWMEKRPPVFKGR"
     gene            1576..2466
                     /locus_tag="VFX21_04740"
     CDS             1576..2466
                     /locus_tag="VFX21_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095293.1"
                     /translation="MVRSRRLRQAGIALWVGALALIMFGLAAPDRAFAQSPSDPRAAF
                     FDGNVTTCAEVGLASDAQVGSPTGGNASDANVSGVVTTNAGTVQPGQGEEVNVTITGS
                     SVVIDAIVVKGGNAYNVYSNPAVLPPALAPPQHYISPLTGGGQVPELSHWFVCYHIGT
                     QPTTTTTSNATSTSTTSMSTTSTSTTSTSTTSTTSTTSTTTTSTTGTTTSSTTTAPTS
                     TSTTDGGAGGLGTTATTGVTTTLVAPTDAANIAGDDANAAASLPRTGDSSVPLVVGFV
                     ALAAGSVLVGAGRRQGAGGR"
     gene            complement(2463..3122)
                     /locus_tag="VFX21_04745"
     CDS             complement(2463..3122)
                     /locus_tag="VFX21_04745"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX5095294.1"
                     /translation="MDETVRDDGFEAVFVVRTPRAEAWEQLCNAKPAFEFFGEARPGQ
                     WWIPGIEAPADELEVKPESLLRARKAVEPCKGTEIVITLEDADTGTRIHIVQTGFGDG
                     FSEQRPWLTAGWHAILADLVVFFERDVSLGRHGTWWYPIGCDVIETDEGLAVANVMPG
                     GFAAQAGMRRGDLILQLAGAPVVNVRDLSILTRGPLKTGTEWGVRYLRDRAVLTGTGV
                     I"
     gene            complement(3122..3703)
                     /locus_tag="VFX21_04750"
     CDS             complement(3122..3703)
                     /locus_tag="VFX21_04750"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5095295.1"
                     /translation="MTAPRVEVIEDPDRIGALAHPTRVAVLDALRSPNTAAGAARALG
                     LTRQAVNYHVKALLDAGLIRLAEERRKGNFIEQVYESVAGAFVVSPRVAWSDRRRADA
                     LTRELPLERLVTLGDALQRDAVELLDRAAFDEEDIPCASVDATVHFRDEAARAAFIDE
                     YLTSLRQLVKKYGTRNGDTYRVAVAVYPNTERS"
     gene            3816..>4732
                     /locus_tag="VFX21_04755"
     CDS             3816..>4732
                     /locus_tag="VFX21_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5095296.1"
                     /translation="MPREFRGTVNLDLRSSQEDWDAFLADRAPAGAPNVLVVLYDDTG
                     QAAWSPYGGRIQMPTMQRLADNGLIYSQWHTTALCSPTRSTMLTGRNHHQNGFASISE
                     TALGFAGYSSHIPPENAPMAHILRDAGWSTYWVGKNHNVPVDEWTMGASKKNWPLGMG
                     YDRFYGFIGGETNQWYPDLAEDNHYIDQPYLPEDGYHLSKDLADKALEFIRDSKQSEP
                     EKPWYLWFCPGANHAPHHAPQEYIDKYKGVFDDGYEAYREWVLPRMIEKGILPEGTEL
                     TPINPMPPDTFVEGDSVRPWATLNDEEKA"
BASE COUNT          782 a         1669 c         1573 g          708 t
ORIGIN      
        1 tggtgatttc ctctcccatc gactcgtaca cgacgggcgg tcccgcagtg aaaatcgcgg
       61 cgcgagtcgt catcaccgag aagtcggaga tcggcgcgac cagcgcgccg tgcccggccg
      121 acgcgccgag cacggccgtc acgagcggca cgcgacccga gcagcgcgct tgggcgagca
      181 tgtcggtggg cgtcggtgcg atgtggggct tgccgtcggc gcgatagccc gcaccctcga
      241 gcaccatgat gcacggcacc cggtcgcgca ccgcgatgtc ggcgacgcga tagcgcttcg
      301 cattggccgc gccgctgatc gtgcccgcct tcaccgtgaa gtcctcggcc gcgaccatca
      361 ccgggcggcc gtcgatgcga cccgaaccca tgaccagcgc gtccgcgggc gagtcctcgc
      421 cgccgacgag cgtgccgagc tccaggaacg aaccgggatc gagcaagtgc gcgatgcgag
      481 cgcgcacgtc gagctttccc gcggcgcggt gcttggcgag ccgctcgtcg ccgcccatcg
      541 cgcgcgacgc ggcccggcgc tgcgcgaggt cctcgacgac gtcgtcccag ctctcagaag
      601 tgcgatccgg catggtcgtc accgcccctt gaacacgggc ggtcgcttct ccatccacgc
      661 catcagcgcc tcgcgcaggt cttcggtctg gacgttgacg ctctgcgcga gcgcttcgat
      721 ctcgagggcc tgggcgagtg acatcgcact cgcattgtcg atctcgcgct tggtcgacgc
      781 cagcgcgatc ggcgggcccg ccgcgatcgt cgccacgatc tcccggacgg actcgtcgag
      841 accggcggcg gggaagaccg cgttcacgag cccgagctcg gatgcctggt ccgcgctcac
      901 catcttcgcg gtgaacgcga gctccttggc cttgtgcgcg ccgatgcgtt ggcgcagcag
      961 ccacgacgaa ccgaagtcga ggctgagccc gcgcctggcg aagatcgcag agaagcgggc
     1021 ccgttcggag cagtacgtga tgtcggcggc cagcgcgagg ccgaggccgg cgccgacgca
     1081 cagcccgtcg accttggcga cgaccgggat cgggcagtcg tgcacggcca gcaccacgtc
     1141 ggcgaggacg cgcatcgaat cgaccatggg cggtatcgcc tgcgggccgc cactaccgcc
     1201 accgctaccg ctgccgccgc cgccgagatc ggcgccggtg cagaagtcgc cgttcgcgcc
     1261 ggtcagcacg atcgcgcgca cacccgcgtg gctcaggtcg cgaaacgcgg caccgatctc
     1321 cacccacatg tcgcccgtgc acgcgttctt cgcttcgggc cggtcgagcg tcaccgtcgc
     1381 gacgaggtcg tcgcgctcga cccggatgtt cgggtggctc acgccgagat atctataggt
     1441 tttccgcgga tgtggtcaac ttggcaccgg acgacgcggt gtcgccggaa tgaccggaat
     1501 ccgcccatgc gctgaatcga tcgcctgccg gggcgatacc tggatgtgcg cggcggcaat
     1561 accgtccgtg tcgcgatggt gcgttcacgc cggctccggc aagcaggcat cgcgctctgg
     1621 gtgggcgcgc tcgctctcat catgttcggg ctcgccgcgc ccgatcgcgc gttcgcgcag
     1681 tcgccctccg atccgcgtgc cgcgttcttc gacggcaatg tcacgacctg cgcggaggtc
     1741 ggcctcgcga gtgatgcgca ggtcggttca ccgacgggcg ggaacgccag tgacgcgaac
     1801 gtgtcgggtg tcgtcacgac caatgccggc accgtgcaac ccgggcaagg cgaggaagtg
     1861 aacgtcacga tcaccgggag cagcgtcgtc atcgacgcga tcgtcgtgaa gggcggcaac
     1921 gcgtacaacg tgtactcgaa cccggcggtg ctgccgccgg cattggcgcc cccgcagcac
     1981 tacatctcac cgctgaccgg cggcggccag gtgccggagc tcagccactg gttcgtctgc
     2041 taccacatcg gtacgcagcc gaccaccaca accacgagca acgcgacctc gacgagcacg
     2101 acgtccatga gcacgacgtc cacgagcacg acgtccacga gcaccacgtc cacgacgtcg
     2161 acgacatcga cgacgacaac ctcgacgaca gggacgacaa cgagctcgac caccaccgcc
     2221 ccgacgagta cgtcgacgac cgacggcggc gcgggagggc tcggcacgac cgcgacgacc
     2281 ggcgtcacca cgactctcgt cgcgccaacc gacgccgcga acatcgccgg cgacgacgcg
     2341 aatgctgcgg cgtcgctccc gcggaccggc gattcatcgg tgccgctcgt cgtcggtttc
     2401 gtcgcgctcg ccgcgggctc agtgctcgtc ggcgccggtc gccgccaagg cgccggcggc
     2461 cgttagatca cgccggtgcc ggtcagcact gctcggtcgc gcaggtagcg cacgccccac
     2521 tcggtcccgg tcttgagcgg cccacgcgtg aggatcgaca ggtcgcgcac gttgaccacg
     2581 ggtgcacccg cgagctggag gatgagatcg ccgcgccgca tgcctgcctg cgcggcgaag
     2641 ccgcccggca tcacgttcgc caccgcgagc ccttcgtcgg tctcgatcac gtcgcaaccg
     2701 atcgggtacc accaggtgcc gtggcgcccg agtgagacgt cgcgctcgaa gaacacgacg
     2761 aggtcggcga ggatcgcgtg ccagccggcc gtcagccacg gtcgctgctc ggagaatccg
     2821 tcaccaaagc cggtctggac gatgtgaatg cgcgtgcccg tgtcggcgtc ctccagcgtg
     2881 atgacgatct cggtgccctt gcagggctcg acggccttgc gtgcccgcag caacgactcc
     2941 ggtttcacct cgagctcgtc ggccggcgct tcgatgccgg ggatccacca ttgtcccggg
     3001 cgcgcttcgc cgaagaattc gaacgcgggc ttcgcgttgc acagttgctc ccacgcttcc
     3061 gcgcgcggtg ttcgcacgac gaagaccgcc tcgaacccgt cgtcacgaac ggtctcgtcc
     3121 atcagcttct ctccgtgttc gggtacaccg cgacggcgac gcgatacgtg tcgccgttgc
     3181 gcgtgccgta cttcttgacg agttggcgca gcgaggtgag gtattcgtcg atgaacgcgg
     3241 cccgtgccgc ctcgtcgcgg aagtgcaccg ttgcgtcgac ggatgcgcac gggatgtctt
     3301 cctcgtcgaa cgcggcgcgg tcgaggagct cgacggcgtc gcgttgcagc gcgtcgccca
     3361 atgtcaccaa gcgttcgagc ggcaactccc gtgtcagcgc gtccgcgcgc cggcgatcac
     3421 tccacgcgac gcgcggcgac acgacgaacg cgcccgccac cgactcgtac acctgctcga
     3481 tgaagttgcc cttgcggcgt tcctcggcga gccggatgag gcccgcgtcg agcagcgcct
     3541 tcacgtggta gttgaccgct tggcgcgtca ggcccagcgc ccgggcggcg ccggccgcgg
     3601 tgttcggcga ccgcagcgcg tcgagcaccg cgacccgggt cgggtgggcg agcgcgccga
     3661 tccggtcggg atcctcgatg acttccacgc gcggcgccgt cacgaaagaa agtgttgtct
     3721 agaaagggaa tgctgtcaac cctgagcggg tgaagatcgg cttgccggaa cggtcctaag
     3781 agagaggaga aagcccatcg gcgaaggagc acggcatgcc cagggagttc cgcggcacgg
     3841 tcaacctcga cctgcggtcg tcacaagagg actgggatgc gttcctcgcc gatcgcgcgc
     3901 ccgcgggcgc gccgaacgtg ctcgtcgtcc tctacgacga caccgggcaa gcagcgtggt
     3961 cgccgtacgg cggccgcatc cagatgccga ccatgcaacg gctcgcggac aacggcttga
     4021 tctactcgca gtggcacacg accgcgctct gctcgccgac gcgctccacg atgctcaccg
     4081 gccgcaacca tcaccagaac gggttcgcgt cgatctccga aactgcgctc ggcttcgccg
     4141 gttacagctc gcatatcccg ccggagaacg cgccgatggc gcacatcctg cgcgatgcgg
     4201 gctggagcac gtactgggtc ggcaagaacc acaacgttcc cgtcgacgag tggacgatgg
     4261 gcgcgtcgaa gaagaactgg ccgctcggca tgggttacga ccgcttctac ggcttcatcg
     4321 gcggcgagac gaaccagtgg tatcccgacc tcgcggaaga caaccactac atcgaccagc
     4381 cgtacctccc cgaagacggc taccacctct ccaaggatct cgcggacaag gcgctcgaat
     4441 tcattcgcga ctcgaagcag tcggagcccg agaagccgtg gtacctctgg ttctgcccgg
     4501 gcgcgaacca cgcgccgcat cacgcgccgc aggagtacat cgacaagtac aaaggtgtgt
     4561 tcgacgacgg ctacgaggcg taccgcgagt gggtgctccc ccgcatgatc gagaagggca
     4621 tcctgcccga gggcaccgag ctcacgccga tcaacccgat gccgccggat acgtttgtcg
     4681 aaggcgacag tgtccggccc tgggccacgc tgaacgacga ggagaaggcg tt
//
LOCUS       DASUGA010000241         2333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_306307, whole genome shotgun sequence.
ACCESSION   DASUGA010000241 DASUGA010000000
VERSION     DASUGA010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306307"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(419..850)
                     /locus_tag="VFX21_04760"
     CDS             complement(419..850)
                     /locus_tag="VFX21_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014444006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095297.1"
                     /translation="MNRTVVRVGLAVLAVQAAMLGVWAGLAPRSFYDDFPGLGRHWVS
                     IDGPYNEHLVRDVGWLNLALLVVTVWALVTLARSLVIATLVAWLVADLPHLVYHAANL
                     DGLSSSDGTAEIISLALGPAIAAALLVLVVRSDDAASTAST"
     gene            878..1330
                     /locus_tag="VFX21_04765"
     CDS             878..1330
                     /locus_tag="VFX21_04765"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5095298.1"
                     /translation="MTSVYPREEIDAEFAKYVERGKANDWSAWADQFTEDARYVEHEY
                     GIYEGREAIREWIVATMASVSGMSFPIEWYVIDGNRVIMYCWNVFDPLPGMTGEYKFA
                     VVGILEYAGNGQWSLEEDVYNAKEAEQVLVRFLEDAAAAGHAPPSPEL"
     gene            1410..2159
                     /locus_tag="VFX21_04770"
     CDS             1410..2159
                     /locus_tag="VFX21_04770"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX5095299.1"
                     /translation="MSTHRFTRRAVTVGATALAAISFLVGSAAPASAQKTDATGSADQ
                     SRSIVATGTSRVRGTPDVLTVQVGATSRGHTVGEALDRNNAAVRKVIAALVDGGVDKK
                     DIQTTNLSVGPIYGDNGNDIQGYQVSNVVVAHLRDLEKAGSLLDEAVQAGGDDVVLRG
                     VSFDIDDTSDLVAAARVDAVKRARNQAEQLAAAAGVQLGDVMTISESSRDVGPVVSAP
                     DAAASTSVERLVIQSGSQELTVQVSVVFSIR"
BASE COUNT          386 a          827 c          804 g          316 t
ORIGIN      
        1 cggtcgcgtc gtcggcgcgc gagcggtcgc ctgcggcgga gtcggtgccg ccgtcgcacg
       61 cgctggcgac gagcgcgagc accgcgacga gcccgacggc cacaagctgg gcgcggtggg
      121 cgcggtggag ccggcggcga cggtggcgaa tccccccagc tgccatcgac tgtccccgtc
      181 ctcgtcgtgc cgcgggcgcg cggccgctcg ctcttgcgat gatcggcccg cccggtcgcc
      241 cagttgaggc tcggcggcga gggtagcccg ggtcggcagc tggttgaccg acgccctacc
      301 ctgtccgact caccgaggag ccgtcgtgtc caagaagacg aagaagcgca agctcaacgc
      361 ccgccgcaag aaggcgaacc acggcaagcg acccaacgcc ggccgtcgct gagcggcgct
      421 acgtcgaggc cgtgctcgcg gcgtcgtcgc tgcgcaccac cagcaccaac agcgcggcgg
      481 cgatcgcggg gccgagcgcc aacgagatga tctcggccgt gccgtcgctc gacgacaggc
      541 cgtcgaggtt cgcggcgtgg tacacgaggt gtggcaggtc ggccacgagc catgccacca
      601 gtgtggcgat cacgagcgaa cgcgcgagcg tgacgagcgc ccacaccgtc acgacgagca
      661 gcgcaaggtt cagccagccg acgtcgcgca cgaggtgctc gttgtacggg ccgtcgatcg
      721 acacccagtg gcgcccgagc ccggggaagt cgtcgtagaa ggatcgcggc gccaggccgg
      781 cccacacgcc gagcatcgcg gcttgcaccg cgagcaccgc cagacccacg cgtacgaccg
      841 tgcggttcac gcgcgaagca gcgtacgctc cgccttcatg acctctgtgt atccccgcga
      901 agagatcgac gccgagttcg cgaagtacgt cgaacgcggg aaggccaacg actggagcgc
      961 gtgggccgac cagttcaccg aagacgcgcg ctacgtcgag cacgagtacg ggatctacga
     1021 ggggcgcgag gcgattcgcg agtggatcgt cgcgacgatg gcatcggtgt cggggatgag
     1081 cttcccgatc gagtggtacg tcatcgacgg caaccgcgtg atcatgtact gctggaacgt
     1141 cttcgacccg ctcccgggca tgaccggcga gtacaagttc gcggtcgtcg gcatcctcga
     1201 gtacgcgggc aatggtcagt ggtcactcga ggaggacgtg tacaacgcga aggaagccga
     1261 gcaggtgctc gtgcgcttcc tcgaggacgc ggccgccgcc ggccacgcgc caccgtcgcc
     1321 ggagctctga cgcgcgcttt tcgcatacaa ggcgggaact gacgcgcgcg cgccgtcgtc
     1381 caacctccgc aaaccaggag gttcggaaga tgtccacgca ccgcttcacc cgtcgcgccg
     1441 tcaccgtcgg tgccaccgcg ctcgctgcga tctcgttcct cgtcggttcg gcggcgccgg
     1501 cgtcggccca gaagaccgat gccaccggtt cggccgacca gtcgcgctcc atcgtcgcca
     1561 ccggcacctc gcgcgtccgt ggcacgcccg acgtcctcac ggtgcaggtc ggcgccacga
     1621 gccgcggtca cacggtcggc gaagcgctcg accgcaacaa cgccgccgtg cgcaaggtga
     1681 tcgcggcact cgtcgacggc ggcgtcgaca agaaggacat ccagacgacg aacctgtcgg
     1741 tcgggcccat ctacggcgac aacgggaacg acatccaggg ctaccaggtg agcaacgtcg
     1801 tggtggcgca cctccgcgat ctcgagaagg cggggagcct cctcgacgag gccgtgcaag
     1861 cgggcggcga cgacgtcgtg ctccgcggcg tgtccttcga catcgacgac accagcgacc
     1921 tcgtggccgc ggcgcgcgtg gacgcggtga agcgggcccg caaccaggcc gagcaacttg
     1981 cggccgcagc cggcgtgcag ctcggcgacg tgatgaccat cagcgagtcc agtcgcgatg
     2041 tcggccccgt ggtcagcgcg ccggacgccg ccgcctcgac gtcggtcgag cgcctggtga
     2101 tccagagcgg atcccaagag ctcaccgtgc aggtgtccgt ggtgttctcg atccggtagc
     2161 cgcgcgcact tcgcgcgcta ccagacgatg cgcacaccgc gccgagtggt gacgccacca
     2221 cttggcaccc ttcccgcgac cgccggtggg acaatggtgc gcctcgcagc cgccgaacgg
     2281 gcagacacgt gctcttcccc accgtcacct tcgccatctt cttcctgctc gtg
//
LOCUS       DASUGA010000242         2174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1965241, whole genome shotgun sequence.
ACCESSION   DASUGA010000242 DASUGA010000000
VERSION     DASUGA010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1965241"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VFX21_04775"
     CDS             <1..188
                     /locus_tag="VFX21_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011981421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX5095300.1"
                     /translation="CTSCGHVGCCDNSPGKHATAHFHEQEHPIVQSFEPGENWYWCYV
                     DDLGFERDGETSSPSHP"
     gene            199..1056
                     /locus_tag="VFX21_04780"
     CDS             199..1056
                     /locus_tag="VFX21_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095301.1"
                     /translation="MTDWFEITKRNARSVQTTIGWIFWDPGAVARYEKHGLLGPLGYI
                     AARAAPLAPAGADATIAAFASISPVGIRLAFDLVAQTTSFDAVWKMRDEAVAEGLRAH
                     APAIVEPLTELGPALWPVVEQLPAAGRVFFAAHLRMPRPDDPLLSGWHAVNCIREWRG
                     DTHWALVTAAGIEGVGASILHNAWLGYERDWLAKSRGATEEEIARAWAALTDRGLARD
                     GEVTPEGIALRQRIEDDTDRLTTRPWELLGEDRARRFAAEFEPPCELLLHRVDVTAGP
                     NYQPGSRIR"
     gene            complement(1073..>2174)
                     /locus_tag="VFX21_04785"
     CDS             complement(1073..>2174)
                     /locus_tag="VFX21_04785"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX5095302.1"
                     /translation="AGCHEHPLATRDGVASGQVSAPSHQAPSHIVFELTATDAAGATA
                     TVSRSVDPNAVALTFQTLPPGLSLTVGPEDATAAPFTQTWAANSQLQLSAPAQQTVGG
                     TTYQFAGWSQGGTATQSIFVPSVNTTYTATYTSVGCSGPSYASAVLADAPRAYWRLGD
                     TSGTTAADSSPNAYSGTYVGGVTLNAAGALTGIANPAATFDGANDHMIRNPFPGMPTT
                     AMTVDLWIKTNDTTKEAGIASYASTASVDEFQLRDQRNLKVYIRGTSVTTGVVLNDGN
                     WHHLAVTWTSAGGALAVYKDGALAFTGTLRAGYAMTTGGAWILGQEQDALGGGFETSQ
                     AYLGTLDEVAVYPSALPAARVQAHYNARSGSC"
BASE COUNT          313 a          744 c          769 g          348 t
ORIGIN      
        1 gctgcacgtc ctgtggccat gttgggtgtt gcgacaactc gcccggcaag catgcgaccg
       61 cgcatttcca cgagcaggag cacccgatcg tccagtcgtt cgaaccgggt gagaactggt
      121 actggtgtta tgtcgatgat ctcggcttcg agcgcgacgg cgagacgtcg agcccatcgc
      181 acccttagga agccgggcgt gacggattgg ttcgagatca ccaagcggaa cgcgcgatct
      241 gtgcagacga cgatcggctg gatcttctgg gatccgggcg cggtcgcgcg ctacgagaag
      301 cacggcctgc tcggcccgct cgggtacatc gcggcgcgcg ccgcgccgct cgcgcccgcg
      361 ggtgccgacg cgacgatcgc ggcgttcgct tcgatcagcc cggtcggcat ccgcctcgcg
      421 ttcgacctcg tcgcgcaaac gacgtcgttc gacgcggtgt ggaagatgcg cgacgaagcg
      481 gtcgccgaag gactgcgtgc gcacgcgccc gcgatcgtcg agccactgac cgagctcggc
      541 ccggcgttgt ggccggtcgt cgagcaactg ccggcagccg gtcgcgtgtt cttcgccgca
      601 cacttgcgca tgccgcgccc cgacgatccg ttgttgtcgg gttggcatgc cgtcaactgc
      661 atccgcgagt ggcgcggcga cacgcactgg gcgctcgtga ccgccgccgg catcgaaggt
      721 gtcggcgcct cgatcctgca caacgcgtgg ctgggctacg aacgcgactg gctcgcgaag
      781 tcgcgcggcg ccaccgagga agagatcgcg cgcgcatggg ccgcgctcac cgatcgcggg
      841 ctcgcccgcg acggcgaggt gacacccgag ggcatcgccc tacggcagcg aatcgaggac
      901 gacaccgacc gtctgacgac ccgtccgtgg gagctgctcg gcgaagaccg ggctcgccgc
      961 ttcgcggcgg agttcgagcc gccgtgcgaa ctgttgctgc accgcgtcga cgtcaccgcg
     1021 ggccccaact accagcccgg gtcccgcatc cgctgacccg cgttcgcgct cgctaacagc
     1081 tgccggaccg ggcgttgtag tgggcttgca cgcgcgccgc ggggagcgcg gacgggtaga
     1141 ccgcgacctc gtcgagggtg ccgaggtacg cctgtgacgt ctcgaagccg ccgcccagcg
     1201 cgtcttgctc ctgaccgaga atccatgcgc cgccggtggt catcgcgtag ccggcgcgca
     1261 gcgtgccggt gaacgcgagc gcaccgtcct tgtagaccgc cagcgcaccg ccggcactcg
     1321 tccacgtgac cgcgaggtga tgccagttgc cgtcgttgag cacgacaccg gtcgtgaccg
     1381 acgtgccgcg gatgtagacc ttcaggttcc tctgatcgcg cagctggaat tcgtccaccg
     1441 acgccgtcga cgcgtacgag gcgatgcccg cttccttcgt cgtgtcgttc gtcttgatcc
     1501 aaagatcgac cgtcatcgcg gtggtgggca tgccggggaa cgggttgcgg atcatgtgat
     1561 cgttggcgcc gtcgaacgtc gccgccgggt tcgcgatgcc ggtcagcgcg cccgcggcgt
     1621 tgagcgtgac gccaccgacg taggtgccgg agtacgcgtt ggggctgctg tcggcggccg
     1681 tggtgccgct cgtgtcgccg agccgccagt acgcgcgcgg cgcatcggcc agcaccgcgc
     1741 tcgcgtacga cgggccgctg cagccaacgg atgtgtacgt cgcggtgtac gtcgtgttca
     1801 ccgacggcac gaagatcgac tgagtcgcgg taccgccctg tgaccaaccg gcgaactgat
     1861 acgtcgtgcc gccgaccgtc tgttgcgcgg gcgcgctgag ctgcagctgc gagttggccg
     1921 cccacgtctg cgtgaacggc gcggcagtcg catcctccgg cccgaccgtc aacgacagtc
     1981 ccggcggcaa ggtctggaac gtcaaagcca ccgcgttcgg gtcgacgctg cggctgacag
     2041 tcgcggtcgc gccggcggcg tcggtcgcgg tgagctcgaa cacgatgtgc gacggcgcct
     2101 gatggctggg cgcggagacc tgcccgctcg ccacgccgtc acgcgtggcg agcggatgtt
     2161 cgtggcagcc ggct
//
LOCUS       DASUGA010000243         5312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1278641, whole genome shotgun sequence.
ACCESSION   DASUGA010000243 DASUGA010000000
VERSION     DASUGA010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278641"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VFX21_04790"
     CDS             complement(<1..337)
                     /locus_tag="VFX21_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020520311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5095303.1"
                     /translation="MGETVSFASNGDTASGYLAKPASGTGPGVIVLQEWWGLAPQLKR
                     VCDRLAAEGFVALAPDLYRGDIAEHTEMDKAGQLMSTLPPDRAAADMEGAVDYLRGLP
                     SVQNDKIGVI"
     gene            complement(337..801)
                     /locus_tag="VFX21_04795"
     CDS             complement(337..801)
                     /locus_tag="VFX21_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012786391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5095304.1"
                     /translation="MAGKRDRIKMTDAEIDAFIDQERTLQVASINKDGTPHLVAMWFA
                     RFEGKLAFWTYGKSQKVLNVQRDPRLTVMLESGDKYEELKGVTIYGNARVISDLDVVL
                     QFGEAVYERYWGPIDSDLVREGVRTMGAKRVLIVVEPDKIVSWDHSKLGGAY"
     gene            882..1367
                     /locus_tag="VFX21_04800"
     CDS             882..1367
                     /locus_tag="VFX21_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095305.1"
                     /translation="MAQNPDPALALTSLKGMTRTLDDWATVFNLAIVVLPPRPEAAGF
                     VPVIDRIFKTFGDSDVRTCVCVAGSEAIARRILGPDADRWLVFADSDGTLPSSLGLER
                     LPAFVHLRQDTTLAAAAQGWSPTEWQKIADQIARSLHWTSPIVSGRNDPPPSPGWPIA
                     S"
     gene            1396..2574
                     /locus_tag="VFX21_04805"
     CDS             1396..2574
                     /locus_tag="VFX21_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX5095306.1"
                     /translation="MLPVWRRLAPRTMVVGVVVAALSVPLALGAPAGASPQDDLEHER
                     ATAEQLEAKIDDLNQRISMLDENYNEGQLEISQTTANIEDAQHRIDDAVARSDGLSAR
                     LRNRAALLYVQASDTTPIDALEAANVHEIGSRSKYGAAAAEQDATLIDDLSVARQDLA
                     DQQAALEQQRKDAETKQSELQQLRDQMSDAQSQQEELLGQAQGRIAGLVAEIEAQRRA
                     EEEARAKAELEKKQREEAAAKAAAAAATTAGTRGTSSTSAKKSAAVNAPDPGSRAVAS
                     SPNAQAAVDAAYGELGKPYRYAGDGPENFDCSGLTMFAWAAAGVSLPHSSRAQYAALP
                     HVSIDALEPGDLVFYGNPIHHVGIYIGNGQYIHAPQTGDIVKISGIYRDDYTGGARPG
                     "
     gene            2599..3654
                     /gene="selD"
                     /locus_tag="VFX21_04810"
     CDS             2599..3654
                     /gene="selD"
                     /locus_tag="VFX21_04810"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013916318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX5095307.1"
                     /translation="MTDTEAIRLTSFSHGAGCACKLSPSDLRTVLGLVRGFEPVTDPN
                     LLVGFDTADDAAVYRLRDDLAIVVTTDFFTPIVDDPFDWGRIAATNALSDVYAMGGTP
                     LLALNLVAWPREALPFELLARVLDGGAAVARDAGCIIAGGHSIDDAEPKYGLAVVGTV
                     DPRAVLANSAAQPGDALVLTKPIGLGAISTAVKREQAAPALVQRAIEVMTTLNASARD
                     AALEVGVHAATDVTGFGLVGHLRELCSGSGVGAVVDAAAVPVIDGVRELVAAGMVAGG
                     TQRNHAFVAPDIDFGSLPEPDQLLLCDAQTSGGLLLSVAAAKADRLLGALRERGTLAA
                     EVIGLTTAAHPGRITVK"
     gene            complement(3765..4766)
                     /gene="trxA"
                     /locus_tag="VFX21_04815"
     CDS             complement(3765..4766)
                     /gene="trxA"
                     /locus_tag="VFX21_04815"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX5095308.1"
                     /translation="MALVTNIVNGSNAARLLVLLHGYGADEVDLGGLLPYLDPDGTFA
                     TVLPRAPFAAPGTPGFAWYDFGGDANVIQQGYLEAIALVDELVDEQCATLGMERSDAI
                     FAGFSQGAGVALGLGLLPSDRPLPAAILAMSPATPIPDAFTELPEGARDVPVLVQHGT
                     DDPLIPVQRSRDFARQLRSVGVPTVYREYPMQHQVAMESMRDARDWLTRVLEGERPDE
                     AVPEDPVELVPSMTTARWEAEVLGSELPVIVDFWAPWCGPCRQVSPVVETIAAMRKGS
                     YKVVKVNIDDEPQIAQQYDVQSIPMIGLFRNGRMERASVGAKPRQQLEAELGMLVIP"
     gene            4813..>5312
                     /locus_tag="VFX21_04820"
     CDS             4813..>5312
                     /locus_tag="VFX21_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX5095309.1"
                     /translation="MQLGMIGLGRMGANIVRRLMRDGHTCVVYDRDDSVVASLAAEGA
                     VPTGSLAELSEKLDPPRAVWVMVPAGITGAVVDNVAATLAPGDIVIDGGNSNYRDDLT
                     RAAELATKGIEYVDCGTSGGVFGLERGYCLMVGASDAAFARIEPILRTLAPGIETAPR
                     TTGRTG"
BASE COUNT          850 a         1881 c         1804 g          777 t
ORIGIN      
        1 cgatcacgcc gatcttgtcg ttctgcaccg acggcaaccc gcgcaggtag tcgacggcgc
       61 cttccatgtc ggcggccgcg cggtcgggcg gcaacgtcga catgagctgg ccggccttgt
      121 ccatctccgt gtgctcggcg atgtcgcctc ggtacaggtc gggcgcgagc gcgacgaagc
      181 cttcggcggc caagcggtcg cacacgcgct tgagctgcgg tgcgagtccc caccactcct
      241 gcaagacgat cacacccgga cccgtgcccg atgccggctt cgcgaggtaa ccgctcgcgg
      301 tgtcgccgtt gctcgcgaac gaaacggtct cacccatcag tacgcacccc cgagtttcga
      361 gtggtcccac gacacgatct tgtcgggctc cacgacgatc aacacgcgct tggcacccat
      421 tgttcgtaca ccctcgcgaa ccagatcact gtcgatcggg ccccaatacc gctcgtacac
      481 ggcttcgccg aactgcaaca cgacgtcgag atccgagatg acgcgcgcgt tcccgtagat
      541 ggtgacgccc ttgagctcct cgtacttgtc gccgctctcg agcatcacgg tcaaccgagg
      601 gtcgcgctgc acgttcagca ccttctgcga cttcccgtac gtccagaacg cgagcttgcc
      661 ctcgaaccgg gcgaaccaca tcgccacgag gtggggcgtg ccgtccttgt tgatggacgc
      721 cacctgcaac gtacgttctt ggtcgatgaa cgcgtcgatc tcggcgtcgg tcatcttgat
      781 gcggtcacgc ttgccggcca tgaccgggac aatacgaggt ggccgctcga cggcgcccgg
      841 tggccgcttg cacacctcgt ccggagtagg ttctccgccc aatggcgcag aacccggacc
      901 cggcgctcgc cctcacgagc ctcaagggca tgacccgcac gctcgacgac tgggcgaccg
      961 tcttcaacct cgcgatcgtc gtgctgccgc cgcggcccga ggcggccggg ttcgtgccgg
     1021 tgatcgaccg gatcttcaag accttcggcg acagcgacgt ccgtacgtgc gtctgcgtcg
     1081 cgggctcgga ggcgatcgcc cgccggatcc tgggtccgga cgcagatcgt tggctggtgt
     1141 tcgcggacag tgacgggacg ctgccgtcca gcctcgggct cgagcggctg ccggccttcg
     1201 tccacctgcg ccaggacacc accctggctg ccgcggccca gggctggagc ccgaccgaat
     1261 ggcagaagat cgccgaccag atcgcgcgct cgctccactg gacgtcaccc atcgtgagcg
     1321 gccgcaacga cccgccgccc tcaccgggct ggccaatcgc aagctgagcc gcgccgcgag
     1381 ctaggtttga ggcccatgtt gcccgtgtgg cgccggctgg cgccccggac catggtggtg
     1441 ggggtcgttg tcgccgcctt gtccgttccc ctcgcgctcg gcgcgcccgc gggcgcgtcc
     1501 ccgcaggacg atctcgagca cgagcgcgcg accgcggaac aactcgaagc gaagatcgac
     1561 gacctgaacc agcgcatctc gatgctcgac gagaactaca acgagggcca gctcgagatc
     1621 tcgcagacga cggcgaacat cgaggacgcg cagcaccgta tcgacgacgc cgtggcccgc
     1681 tccgacggac tcagcgctcg cttacgcaac cgggccgcgc tgctctacgt ccaggcctcc
     1741 gacaccacgc cgatcgacgc gctcgaagcc gcgaacgtgc acgagatcgg gtcgcgctcg
     1801 aagtacggcg ccgccgcggc cgagcaggac gcgacgctca tcgacgacct gagcgtcgcc
     1861 cgccaggacc tcgccgacca gcaggcggcg ctcgagcagc agcgcaagga cgccgagacc
     1921 aagcagagcg agctgcagca gctgcgcgac cagatgagcg acgcgcagtc gcagcaagag
     1981 gagttgctcg gccaggccca gggccgcatc gccgggctcg tcgccgagat cgaagcacaa
     2041 cggcgcgccg aggaagaagc gcgcgccaag gccgagctcg agaagaagca gcgcgaggaa
     2101 gcggccgcga aggcggccgc cgcggcggcg accaccgcgg gcacacgcgg gacgtcgtcg
     2161 accagcgcga agaagtcggc cgcggtgaac gcccccgacc caggttcgcg agcagtcgcg
     2221 tcgagcccga acgcgcaggc cgcggtcgac gccgcctacg gcgagttggg caagccgtat
     2281 cgctatgccg gcgacgggcc cgagaacttc gactgctccg gcctcaccat gttcgcctgg
     2341 gcggcggcgg gcgtgagcct gccgcactcg tcccgggctc agtacgccgc cctgccgcac
     2401 gtgtcgatcg acgcactgga accgggcgat ctcgtcttct acggcaaccc gatccaccac
     2461 gtcgggatct atatcggcaa cggccagtac atccacgccc cgcagacggg cgacatcgtg
     2521 aagatttcgg gcatctaccg cgacgactac acgggcggcg ctcgccccgg atagcggcgg
     2581 gcgcggcaca atcgtgtcgt gaccgacacc gaggcgatcc gtctcacttc cttctcgcac
     2641 ggggcgggtt gcgcctgcaa gctgtcgccg agcgacctgc gtaccgtgct gggtctcgtg
     2701 cgcggcttcg aaccggtcac cgacccgaat ctcctcgtcg gcttcgacac cgccgacgac
     2761 gccgcggtgt accggttgcg cgacgacctg gcaatcgtcg tcaccaccga cttcttcacg
     2821 ccgatcgtcg acgacccgtt cgactggggc cgcatcgcgg cgacgaacgc gctctcggac
     2881 gtctacgcga tgggcggcac gccactgctc gcgctgaacc tcgtcgcgtg gccgcgcgaa
     2941 gcactgccgt tcgaactgtt ggcgcgtgtg ctcgacggcg gcgcggccgt cgcacgcgac
     3001 gcgggctgca tcatcgcggg cggacattca atcgacgacg ccgagccgaa gtacggcctc
     3061 gcggtcgtcg ggacggtcga tccgcgcgcg gtgctcgcga acagtgcggc gcaaccgggc
     3121 gacgcgctgg tgctcaccaa gccgatcgga ctcggcgcca tctcgaccgc agtgaagcgt
     3181 gagcaggcgg cgcccgcgtt ggtccagcgc gcgatcgagg tgatgacgac gttgaacgcg
     3241 tcggcgcgcg acgccgcgct cgaggtgggc gtgcacgcgg cgaccgacgt gacgggattc
     3301 gggttggtcg ggcacttgcg cgagctctgc agcggttcgg gcgtcggtgc tgtcgtcgac
     3361 gcggcggcgg tgccggtcat cgacggcgtc cgcgagctcg tcgcggccgg gatggtcgcg
     3421 ggcggcacgc agcgcaacca cgcgttcgtc gcgcccgaca tcgacttcgg gtcgttgccg
     3481 gagccggacc agctgctgct gtgcgacgcc cagacctcgg gcggcctgct cctgtcggtc
     3541 gccgcggcca aggccgaccg cctactgggc gccctccgcg aacgaggcac gttggcggcc
     3601 gaagtcatcg gcctgacaac ggctgcccac cccggtcgaa taaccgtgaa gtaagacgcg
     3661 cgcgtcgcac gaggcggtgc cggagcttgc ggaggtactc gcggagcgga gcgcgggcca
     3721 acaatccggg ctgcctcacg cgagcacgtg cggagcgaca agagttacgg gatcaccaac
     3781 attccgagct cggcttcgag ctgttggcgc ggtttggcgc cgacggatgc tcgttccatg
     3841 cggccgttgc ggaacaggcc gatcatcggg atgctctgca cgtcgtactg ctgcgcgatc
     3901 tgcggctcgt cgtcgatgtt gaccttcacg accttgtagg agcccttgcg catcgcggcg
     3961 atggtctcga ccaccggcga gacctggcgg cacggtccgc accacggcgc ccagaagtcg
     4021 acgatgaccg ggagctcgct gcccaacact tcggcttccc accgcgcagt cgtcatcgac
     4081 ggcaccagct cgacgggatc ttcggggacc gcttcgtcgg gccgctcacc ctcgagcacc
     4141 cgcgtgagcc aatcgcgcgc gtcacgcata ctctccatcg cgacctggtg ctgcatcggg
     4201 tactcgcggt acaccgtcgg aacgccgacg ctgcgcaact ggcgcgcgaa gtcgcgcgac
     4261 cgttgcaccg ggatgagcgg atcgtccgtg ccgtgttgca cgagcaccgg gacgtcccgc
     4321 gccccctccg gcagctcggt gaacgcgtcc ggaatgggtg tggcgggact catcgcgagg
     4381 atcgcggccg gcaggggccg gtcgctcgga agcaagccga gcccgagcgc gactccggcg
     4441 ccttgcgaga agccggcgaa gatcgcgtcg ctgcgttcca tgccgagcgt cgcacactgc
     4501 tcgtcgacca actcgtcgac cagcgcgatc gcctcgaggt aaccctgctg gatcacgttg
     4561 gcgtcgccgc cgaagtcgta ccacgcgaat cccggcgtgc cgggcgccgc gaacggcgcg
     4621 cgcggcaaca ccgtcgcgaa cgtgccgtcg gggtcgaggt acggcaggag gccgccgagg
     4681 tcgacctcgt cggcgccgta cccgtgcagc agtacgagca ggcgagcggc gttcgagccg
     4741 ttcacgatgt tcgtcacgag ggccattggg tcagactact gacctcgcac accggactcc
     4801 ctgggggcgt cgatgcagtt gggaatgatc gggttggggc gcatgggcgc gaacatcgtg
     4861 cgccgcctca tgcgcgacgg gcacacatgc gtcgtctacg accgcgacga cagtgtcgtt
     4921 gcgtcgctcg ccgcggaagg cgcggtgccc accggatcac tcgcggagct gagcgaaaag
     4981 ctcgacccgc cgcgcgcagt ctgggtgatg gtgcccgccg gcatcacggg cgccgtggtc
     5041 gacaacgtcg cggcgacgct cgcgccgggc gacatcgtca tcgacggcgg caacagcaac
     5101 taccgcgacg acctcacacg cgcggccgag ctcgcgacca agggcatcga gtacgtcgac
     5161 tgcggaacga gcggcggtgt gttcgggctc gagcgcggct attgcctgat ggtcggcgcg
     5221 tcggatgccg cgttcgcacg catcgagccg atcctccgca cactggcgcc cggcatcgag
     5281 accgcgccgc gcacgaccgg gcgaaccggc ga
//
LOCUS       DASUGA010000244         2003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_722843, whole genome shotgun sequence.
ACCESSION   DASUGA010000244 DASUGA010000000
VERSION     DASUGA010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722843"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2003
                     /locus_tag="VFX21_04825"
     CDS             <1..>2003
                     /locus_tag="VFX21_04825"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEX5095310.1"
                     /translation="ADARLKLHVTKVYIEAVDANGQDILYPECPTSLECGLTITGTVT
                     FDVKAYRPFITPFFSGKSSVKLSGYQGHWEGRNKVVIDGRQPLWNDDFEIDRDAGGFG
                     SQQHAIYQLKQPITVEIDISHLDVDVPFTLWVEAVAETNNHRQRESYVAAFFRDPIST
                     EGAEIETFGLDPVGGPFTDPLPDEPQAPECSSGDEAAAGTLQFAAATFLAPEMAQAFT
                     QVEITRTGGTLGAVSAKVRTHDDTAHAGEDYETVDTSVYFGPGDDAPRVLEVPLTLDD
                     VAEPDKSLTVTLSDPRGCATLGTQASATLTILDDDRPEPPPPPSGLDETFGSQGKTTL
                     AGFGDETDMAIQPDGKIVMVGGKFIDFVLARFNADGTVDTGFGDEGKIATDLAGGFAQ
                     ERARAVAIQTDGKIVVAGEATVPSGDLAVALVRYNADGTLDTTFGTGGKVFGPAVLGR
                     AFDVAILADSRIVVAGDAPVGNIADDFGDFLLARFQANGVLDTTFGTNGVVVHDMTGR
                     TDLARNLLVQPNGALVVSGDPFGSDPARRTSVARYDADGHLDASFANGGRLIIGGAYV
                     GEGLALQTDGRLVLVGSVQVLNVQNDFTHFAALRLNADGTFDDSFGDGGMATTSITGL
                     TDVAHAVVVQGDGKIVVAGEGNLINANFAVVRYDRNGDLDTSFA"
BASE COUNT          358 a          658 c          667 g          320 t
ORIGIN      
        1 gccgatgcgc gtctcaagct gcacgtgacc aaggtgtaca tcgaggcggt cgacgccaac
       61 ggccaggaca tcctgtatcc ggagtgcccg acgagcctgg aatgcgggct gacgatcacc
      121 ggcacggtga cgttcgacgt gaaggcgtat cggccgttca tcacgccgtt tttttccggc
      181 aagagcagcg tcaagctctc cggctaccag ggtcactggg agggtcgcaa caaggtcgtg
      241 atagatgggc gacagccgct gtggaacgac gacttcgaga tcgaccgcga cgccggcggc
      301 ttcggctcgc agcagcatgc gatctaccag ctcaagcagc cgatcacggt cgagatcgat
      361 atctcgcatc tcgacgtgga cgtgccgttc acgctgtggg tcgaagccgt ggcggaaacc
      421 aacaatcacc ggcagcgcga gtcgtacgtc gcggccttct tccgcgaccc gatcagcacc
      481 gagggcgccg agatcgaaac gttcggcctc gatccggtcg gcggcccgtt caccgacccg
      541 ctgcccgacg agccgcaggc tcccgagtgc agctcgggcg atgaagccgc tgcggggacg
      601 ctgcaattcg ccgcagcgac tttcctcgca cccgagatgg cgcaggcgtt cacgcaagtc
      661 gagatcacgc gcaccggcgg caccttgggt gcggtgagcg cgaaggtgcg tacgcacgac
      721 gacactgcgc atgcgggtga ggactacgag accgtcgata cctctgtcta cttcggcccc
      781 ggcgacgacg caccgcgcgt gctcgaagtg ccgctaacgc tcgatgacgt cgccgagccc
      841 gacaagtcgc tgaccgtgac cttgagcgat ccacggggtt gcgcgacgct cggcacgcaa
      901 gcgagcgcga cgctgacgat cctcgacgac gatcggccgg agccgccgcc gccgccgagc
      961 ggtctcgacg aaacgttcgg ctcgcaaggc aagacgacgc tcgccggctt cggcgacgag
     1021 acagacatgg cgattcagcc ggacggcaag atcgtcatgg tgggcggcaa attcatcgac
     1081 ttcgtgctcg cgcgcttcaa cgccgatggc accgtcgaca cgggattcgg tgatgagggc
     1141 aagatcgcca ccgacctcgc cggcggcttc gcgcaggagc gtgcccgcgc cgtcgcgatt
     1201 cagaccgacg gcaagatcgt cgtcgccggc gaggcgaccg tgccgtccgg cgatctggca
     1261 gtggctctcg tgcgctacaa cgcggacggc acgctcgaca cgacgttcgg tactggcggc
     1321 aaggtgttcg gaccggcggt gctcggtcgt gcgttcgacg tcgcgatcct ggcggatagc
     1381 cgtatcgtcg tagccggcga cgcgcccgtc gggaacatcg ccgacgactt cggcgacttc
     1441 ctgctcgcgc gtttccaggc gaacggtgtg ctcgatacga ccttcggcac caacggggtc
     1501 gtcgtccacg acatgacggg gcgcacggat ctcgcacgca atctgctggt gcagccgaac
     1561 ggcgcgctgg tcgtgagcgg cgatcctttc ggcagcgacc cggcgcggcg caccagcgtc
     1621 gcgcgctacg atgcggacgg tcatctcgat gcgagcttcg cgaacggtgg caggctcatc
     1681 atcggcggtg catacgtcgg agaaggcctc gcgttgcaga cagacggccg cctcgtactc
     1741 gtaggttcgg tgcaggtgct gaacgtgcag aacgacttca cgcacttcgc agcgctgcgc
     1801 ctcaacgcgg acggcacgtt cgacgactcg ttcggcgacg gcggaatggc gacaacctcg
     1861 atcaccggcc tgactgatgt cgcacacgca gtcgtggtcc aaggcgacgg caagatcgtc
     1921 gtcgccggcg aaggcaacct catcaatgcg aactttgctg tcgtgcgcta cgaccgcaat
     1981 ggcgacctcg acacgtcgtt cgc
//
LOCUS       DASUGA010000245         6258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_896890, whole genome shotgun sequence.
ACCESSION   DASUGA010000245 DASUGA010000000
VERSION     DASUGA010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896890"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VFX21_04830"
     CDS             complement(<1..515)
                     /locus_tag="VFX21_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HEX5095311.1"
                     /translation="MSDNSLPTFEPMFGADVDPGPSFAELLRRVAPHQLPGAMIPPGT
                     QASAEIVHGTTVVAIRTADGVVMAGDRRATAGYTIASRRIEKVFPADEYSGVSIAGAA
                     GPAVEMVKLFQTQLEHYEKVQGDALSLEGKANQLGQLVRANLPAAMQGFAVVPLFAGY
                     DHARDRGRVFS"
     gene            complement(595..2136)
                     /locus_tag="VFX21_04835"
     CDS             complement(595..2136)
                     /locus_tag="VFX21_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095312.1"
                     /translation="MLVRRYPVAAATALAALTVSALGWRGRDLPAQLLRISVFRNGDW
                     FWNPQWFGGHPTLGYSVLLPALGAVLGVTIVGVLVTAGAVAAFESLVRHRPRAMLATS
                     VFAFGMLSNLVVGRLSFGLGAAFALAALATHSRGRALPIVFAVAASLSSPVVAVFLAI
                     AFAAWAYTTCQYRNGMVLISAALAPAVVTSVLFGTGGTYPYPFTTFLWSFGLCALTAL
                     ATEDKAIRIGCAMFGAIAFAAFALPTPLGSNVIRMPLLLAAPLVLLADPARTRRIVVA
                     AAVPIAGFLQTAQIAEVAAASTVDPSTDRAYYVPLIEYLQRQDAPIRVEIPPTKQHWE
                     TAYVAELVPIARGWERQIDRGVNPEFYDPAQPLTPARYRAWLQRTGVTHVALSDALPD
                     PWALAETGLVEDGQPYLHEVWHDAHWRVFEVDGASLVDGPARLSDLSDTRAVVDVTGP
                     GEVVVRVRESPYWVVTSGDACMVAGTDGWLHLHVAHPGPVTLDIRMEIASVVPGNDDR
                     LCPGD"
     gene            complement(2162..2728)
                     /locus_tag="VFX21_04840"
     CDS             complement(2162..2728)
                     /locus_tag="VFX21_04840"
                     /inference="COORDINATES: protein motif:HMM:NF014941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VII domain-containing protein"
                     /protein_id="HEX5095313.1"
                     /translation="MKECKRCRQTKPLEEFYENPSGRDGKRPECKECTKQRRKAWYAE
                     NRAREIERVAVWQREKPDKVKANIERFTMSGRRFTMSGRRRVSNRRSHLQRKYRMTLE
                     DFERKLASQGGGCAICGTTTARVDNVDHDHATGKVRGILCFRCNAAIGQLDDDPRRAR
                     ALADYLELHFEGDLIFRELGFALELQPS"
     gene            complement(2772..2966)
                     /locus_tag="VFX21_04845"
     CDS             complement(2772..2966)
                     /locus_tag="VFX21_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013286106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HEX5095314.1"
                     /translation="MPERERKQKPAPKEREEVVEEVPATNERGEKLKEDIDDLLDEID
                     SVLEDNAEEFVRNYVQKGGQ"
     gene            complement(2979..4469)
                     /gene="dop"
                     /locus_tag="VFX21_04850"
     CDS             complement(2979..4469)
                     /gene="dop"
                     /locus_tag="VFX21_04850"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HEX5095315.1"
                     /translation="MAVPKIVGIETEYGVTLRGAADPNPVVASSLLINGYVEQRRIGW
                     DFEDESPGRDARGFARDGAQPPEVETHLVNAVLTNGARYYVDHAHPEYSTPECSDPLE
                     AVLWDKAGERILARSMEAARRLLPPGQEIVVHKNNSDGKGNSYGCHENYLVDRNVPFA
                     MLVRNLVPWFVTRQVFTGAGKIGAENGADAVDYQLTQRADFFEEEVGLETTLKRPIVN
                     TRDEPHADPQKYRRLHVIVGDANLCEVATFLKVGTTAIVLAMIEDNFIDKDLGILNPV
                     PSMRAVSHDPSLRATVDVADLGACTAIELQWEFLRLARKYADETGLEPCGGDEIGNVV
                     LDRWEQTLGALERDPMELDGQLDWVTKWKLLRAYVERDNLEWSDPKIELLSIQYHDVR
                     PDRSLYERLVRAGKVERLVAEPDVIAAMGDPPPTTRAYFRGRCLSEWADSVVAANWDS
                     LVLDVGLDPLRRIPMMEPTRGSKKHVEQLFSEVTTPRQLVERLAAP"
     gene            complement(4480..5568)
                     /locus_tag="VFX21_04855"
     CDS             complement(4480..5568)
                     /locus_tag="VFX21_04855"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX5095316.1"
                     /translation="MHWDALDGLRGLAVLLVVVWHAYRLTLARDMSSLAVPTYLWPAG
                     VARFGVDIFFVLSGMLVVRSWHAARERHDSRTQALRHYWTRRAARILPAYWISLLVLV
                     PVASNGLLGHPKRLFAFATMNQYLRLDMPDRVNTVTWSLTTEWHFYILVPLVAFLLTK
                     VGKWPVLAACVALSVWWYWHTPFDLPQSYLFGRLDQFVAGAIVGLIATQSVRHPLVRV
                     VQWPFVTPAAVAALVAIGTYHGRWLGFGTEERFTDSLVHPAVGLLTALVMLRLMTGSC
                     PPFLRHPVVRWFGTISFGLYLWHYPIFDNGLRWTRDATVLPTSVSSSLALPLLFGAAV
                     GVAWLSYVLVERPFLARGTATRAVPTPR"
     gene            complement(5669..>6258)
                     /locus_tag="VFX21_04860"
     CDS             complement(5669..>6258)
                     /locus_tag="VFX21_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HEX5095317.1"
                     /translation="SNREDLIDPAILRPGRLDVKIKIERPDEDSARQIFHQYLTTRLP
                     LADSELKNAGGDPEGAVATMIKAAVDHMYDTGDDNKFLEVTYQNGDKELLYFKDFSSG
                     AMIENIVRRAKKLSIKRAIAGDERGICTDDLIESIRQEFREHEDLPNTTNPDDWAKIS
                     GKKGERIVYVRTIVNEGQAEKGGRQIERVTTGQYL"
BASE COUNT         1014 a         2057 c         2164 g         1023 t
ORIGIN      
        1 taggagaaca cgcggccgcg atcgcgcgcg tggtcgtagc cggcgaacag cggcacgacc
       61 gcgaagccct gcatcgcggc gggcaggttg gcgcgcacga gctggccgag ctggttggcc
      121 ttgccctcga ggctgagcgc gtcgccctgc accttctcgt agtgctcgag ttgggtctgg
      181 aacagcttca ccatctcgac cgcgggtccg gccgcgcccg cgatggagac gccggagtac
      241 tcgtcggccg ggaacacctt ctcgatgcgg cggctcgcaa tggtgtagcc cgcggtcgcg
      301 cgccggtcac cggccatgac gacgccgtcg gcggtgcgga tcgcgacgac cgtcgtgccg
      361 tgcacgatct cggccgaggc ctgggtgccg ggcgggatca tcgcgcccgg gagttggtgc
      421 ggcgcgacgc ggcggagcag ctccgcgaac gacgggcccg gatcgacgtc ggcaccgaac
      481 atcggctcga acgtcggcaa ggagttgtcg gacatcgggc ccgaccctaa tgggtgggaa
      541 gcgcactgga gcccctttgc cggaggcccc gatgcggagg gagcgggcgg gaagtcaatc
      601 gcctgggcac agccgatcgt cgttccccgg tacgacgctg gcgatttcca tgcggatgtc
      661 gagcgtgacc ggtccggggt gcgcgacgtg cagatgcagc cagccgtcgg tgccggcgac
      721 catgcacgcg tcgccgctgg tgacgaccca atacggcgac tcgcggaccc gcacgacgac
      781 ctcgcccgga ccggtcacgt cgacgacggc gcgcgtgtcg ctgagatcgg agagacgcgc
      841 gggcccgtcg acgaggctcg cgccgtcgac ttcgaacacg cgccagtgcg cgtcgtgcca
      901 gacctcgtgc aggtacggct ggccgtcttc gacgaggccg gtctcggcga gcgcccacgg
      961 gtccgggagc gcgtcggaga gcgcgacgtg cgtgacgccg gtgcgctgca accaggcgcg
     1021 gtagcgcgcg ggcgtgagtg gttgtgccgg gtcgtagaac tcggggttca cgccgcggtc
     1081 gatctggcgt tcccagccgc gcgcgatggg cacgagctcg gcgacgtacg cggtctccca
     1141 gtgctgcttc gtcggcggga tctcgacgcg gatcggtgcg tcttgtcgtt gcaggtactc
     1201 gatgagcggg acgtagtacg cgcggtcggt cgacgggtcg acggtcgacg cggccgcgac
     1261 ctcggcgatc tgcgcggtct ggaggaagcc cgcgatcggc acggccgcgg cgacgacgat
     1321 ccgtcgtgtg cgcgcggggt cggcgagcag gacgagcggt gccgcgagga gcagcggcat
     1381 gcggatgacg ttggagccga gcggcgtggg cagcgcgaac gcggcgaacg cgatcgcgcc
     1441 gaacatcgcg caaccgatgc ggatcgcttt gtcttcggtc gcgagtgcgg tgagggcgca
     1501 caatccgaac gaccagagga acgtggtgaa cggataggga tacgtgccgc cggtgccgaa
     1561 caggacgctc gtgacgaccg cgggtgcgag cgcggcactg atgagcacca tgccgttccg
     1621 gtattggcaa gtcgtgtacg cccacgccgc gaacgcgatc gcgaggaaga ccgcgacgac
     1681 cgggctgctg agcgacgccg cgaccgcgaa cacgatcggc agcgcgcggc cgcggctgtg
     1741 cgtcgcgagg gcggcgagcg cgaacgcggc gccgagcccg aacgacaggc ggccgacgac
     1801 gaggttcgag agcatcccga acgcgaagac actcgtcgcg agcatcgccc ggggccggtg
     1861 gcggacgagc gactcgaagg cagccaccgc gccggcggtg acgaggacgc cgacgatcgt
     1921 gacgccgagc accgcgccca gggccgggag caggacgctg taaccgaggg tggggtgacc
     1981 gccgaaccac tgcgggttcc agaaccagtc gccgttgcgg aacaccgaga tccgcagaag
     2041 ttgcgccggc aggtcccgcc cgcgccaacc caacgccgac accgtcagcg ccgcgagcgc
     2101 cgtagcagcc gcgaccgggt acctccgcac gagcatcacg cccggtctga cgcgcggacg
     2161 ctcaggaagg ttgtagctcg agcgcgaagc cgagttcccg gaagatcagg tcgccctcga
     2221 agtggagctc caggtagtcg gccagtgccc gcgcccgtcg cggatcgtcg tcgagctgcc
     2281 cgatggccgc gttgcagcgg aagcagagga tgccgcggac tttgccggtc gcatggtcgt
     2341 ggtcgacgtt gtcaacccgt gcggtcgtcg taccgcagat cgcgcaccct ccgccctgag
     2401 acgcgagctt gcgctcgaag tcctcgagcg tcatccggta cttccgctgc agatgactcc
     2461 tgcgattgga gacacgtcgt cttcccgaca ttgtgaaccg tcttcccgac attgtgaacc
     2521 gctcgatgtt cgccttcacc ttgtcgggct tctcgcgttg ccaaaccgca acgcgctcga
     2581 tctcacgcgc tcgattctcg gcgtaccaag ctttgcggcg ctgcttcgtg cactctttgc
     2641 actcaggtcg cttgccgtct cgtccactcg ggttctcgta gaactcttcg agtggcttcg
     2701 tctgcctgca gcgcttgcat tccttcacga aggaagtatg cgaagcaggt gtgacagttg
     2761 tatgtagtta cttactgccc acccttctgg acataattac gtacaaactc ctcggcgtta
     2821 tcctcgagga ccgagtcgat ttcatcgagc aagtcgtcga tgtcttcctt gagtttctcg
     2881 ccgcgttcgt tggtcgcggg gacctcttcg acgacttctt cgcgttcctt gggcgccggt
     2941 ttctgtttgc gctcccgctc cggcatcaga ccaacctcct acggcgccgc cagccgctca
     3001 acgagctgcc ggggtgtcgt gacttccgag aacagttgct cgacgtgctt cttgctacca
     3061 cgcgtgggtt ccatcattgg gattcttcgt agcgggtcga gcccgacgtc gagcacgagg
     3121 ctgtcccagt tggccgccac gaccgaatcg gcccactccg acaggcaccg cccgcggaag
     3181 tacgccctgg tcgtgggcgg cggatctccc atcgcggcga tcacgtcggg ttcggcgacg
     3241 agtcgctcga ccttgcccgc gcgcaccagc cgctcgtaca gcgaccggtc ggggcgcacg
     3301 tcgtggtact ggatcgacag cagttcgatc ttcggatccg accactccag gttgtcgcgc
     3361 tccacgtacg cgcgtagcag cttccacttg gtgacccagt cgagctgacc gtcgagctcc
     3421 atcgggtcgc gctccagcgc gcccaacgtc tgctcccacc ggtcgagcac gacgttgccg
     3481 atctcgtcgc cgccgcacgg ctcgagcccg gtctcgtccg cgtacttgcg ggccagccgc
     3541 aggaactccc actgcagttc gatcgcggtg cacgcgccga gatcggcgac gtcgaccgtc
     3601 gcgcgcaggg agggatcgtg cgacaccgcg cgcatcgacg gcaccgggtt gaggatcccg
     3661 aggtccttgt cgatgaagtt gtcctcgatc atggcgagca cgatcgcggt cgtaccgact
     3721 ttcaggaacg tcgcgacctc gcacagattc gcgtcaccga cgatcacgtg cagccggcgg
     3781 tacttctgcg ggtccgcgtg cggctcgtcg cgcgtgttga cgatcgggcg cttgagcgtc
     3841 gtttccaatc cgacttcttc ctcgaagaag tcggcgcgct gcgtgagttg gtagtcgacg
     3901 gcgtcggctc cgttctcggc gccgatcttg cccgcgccgg tgaacacctg gcgcgtcacg
     3961 aaccacggca ccaagttgcg caccaacatc gcgaacggca cgttgcggtc gacgaggtag
     4021 ttctcgtgac agccgtacga gttgcccttg ccgtcgctgt tgttcttgtg gacgacgatc
     4081 tcctggcccg gcggcagcaa ccggcgggcg gcctccatgg agcgggcgag gatgcgctcc
     4141 cccgccttgt cccagagcac ggcctcgagc ggatcgctgc actccggcgt ggagtactcg
     4201 gggtgcgcgt ggtcgacgta gtagcgcgcg ccgttggtga gcaccgcgtt gacgagatgc
     4261 gtctcgacct cgggcggctg cgcgccgtcg cgggcgaagc cacgcgcgtc gcgccccggg
     4321 gattcgtctt cgaagtccca accgatgcgg cgctgctcga cgtaaccgtt gatgagcagc
     4381 gatgacgcga cgacggggtt cgggtccgcg gcgccgcgca acgtcacgcc gtattcggtc
     4441 tcgatgccga cgatcttggg cacggccacg aggggcagtc taccgagggg tagggacggc
     4501 tctcgtcgca gttccgcgtg cgaggaacgg ccgctcgacc agcacgtagg acagccacgc
     4561 gacgccgacc gccgcgccga acaacagcgg caacgccagg gacgacgaca ccgacgtggg
     4621 cagcaccgtc gcgtcacgag tccaccgcag gccgttgtcg aagatcgggt agtgccagag
     4681 gtacaagccg aagctgatcg ttccgaacca acgcacgacg gggtggcgca ggaacggcgg
     4741 gcaggagcca gtcatcagcc gcaacatcac cagcgcggtc aacagcccga ccgccggatg
     4801 caccaacgag tcggtgaagc gctcctcggt gccgaagccg agccaccggc cgtgatacgt
     4861 gccgatcgcg acgagcgccg cgaccgcggc cggcgtcacg aacggccact ggacgacgcg
     4921 cacgagcggg tggcggaccg actgggtcgc gatcagcccg acgatggcgc ccgcgacgaa
     4981 ctgatcgagg cgaccgaaca ggtagctctg aggcaggtcg aacggcgtgt gccagtacca
     5041 ccacacactc agcgcgacgc acgccgcgag caccggccac ttccccacct tggtgagcaa
     5101 gaacgcaacg agcggtacca agatgtagaa gtgccactcc gttgtcaggc tccacgtcac
     5161 cgtgttgacg cggtcgggca tgtcgagccg taggtactgg ttcatcgtcg cgaacgcgaa
     5221 cagccgcttc gggtgaccga gcagtccgtt cgacgcgacc ggcaccagga cgagcaacga
     5281 gatccagtag gcgggaagga tgcgcgccgc gcgccgggtc caatagtggc gcagcgcttg
     5341 cgtacgtgag tcgtggcgtt cgcgcgccgc gtgccaactg cgcacgacga gcatccccga
     5401 gagcacgaag aagatgtcga caccgaagcg cgcgacaccc gcgggccaca ggtaggtcgg
     5461 caccgcgagt gacgacatgt cgcgggccag cgtcaggcgg tacgcgtgcc agacgacgac
     5521 gagcagcacg gccaggccgc ggagaccgtc gagcgcgtcc caatgcaacg tggtcgacgt
     5581 cgcggtgggc gcgggagggc gcgctcgagg cgcggcggaa agcgattgca taccgggggc
     5641 actgcgaatt tacggcgttg cggcgcgtct agaggtattg gccggtcgtg acccgttcga
     5701 tctggcggcc acccttctcc gcctgacctt cgttcacgat cgtgcgcacg tagacgatcc
     5761 gctcgccctt cttacccgag atcttcgccc agtcatccgg gttggtggtg ttgggcagat
     5821 cttcgtgctc gcggaattcc tgacgaatgg attcgatcag gtcgtccgtg cagataccgc
     5881 gttcgtcgcc cgcgatcgcc cgcttgatcg agagcttctt cgcgcgccgc acgatgttct
     5941 cgatcatcgc gcccgacgag aagtccttga agtagaggag ctctttgtcg ccgttctggt
     6001 acgtgacttc gaggaacttg ttgtcgtcgc cggtgtcgta catgtggtcg accgcggcct
     6061 tgatcatcgt cgcgaccgcg ccttcgggat cgccgccggc gttcttcaac tcggagtccg
     6121 cgagcggcaa gcgcgtcgtg aggtactggt ggaagatctg gcgcgcactg tcttcgtcgg
     6181 gacgctcgat cttgatcttc acgtcgaggc gaccgggtcg caggatcgcg gggtcgatca
     6241 ggtcttcgcg gttcgacg
//
LOCUS       DASUGA010000246         3163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_871165, whole genome shotgun sequence.
ACCESSION   DASUGA010000246 DASUGA010000000
VERSION     DASUGA010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871165"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VFX21_04865"
     CDS             complement(<1..219)
                     /locus_tag="VFX21_04865"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5095318.1"
                     /translation="MDFAFSEEQEELRGLAAQILADRADLQQLKARDKSEDWYDRDTW
                     SEFAKANLLGIALPESAGGLGLGYLDLCL"
     gene            complement(237..1415)
                     /locus_tag="VFX21_04870"
     CDS             complement(237..1415)
                     /locus_tag="VFX21_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5095319.1"
                     /translation="MYVGLSKEQEALRDELRAYYDKLLTPEIEDDIRKGEGIGPASKA
                     TWKQMASDGWSGVGWPKEWGGRGLTPIEQFVFFDESMRAGAPVPMLTINSVAPTIMRY
                     GSAEQKQFYVPKILAGEIHFAIGYTEPDAGTDLASLKTRAVRDGDEYVINGQKIFTSL
                     ASGSDYVWLAVRTNTEVKKHKGISIIIVPTDTPGFSAVPIENFGGLNTTITTYDDVRV
                     PAANLVGEENGGWNLITNQLNHERVTLCSSGIIERNLDDVRRWAQETKLADGRRVIDQ
                     EWVQVNLARVHAKLEFLRLANWKVAWLSQTGAPLNPADASSIKVFGTEFYMEAARLLM
                     EVMGDAATLSLDSPGSVLRGRVERLVRSMHILTFGGGTNEMQRDLIAIFGLNMPGSPR
                     "
     gene            complement(1444..1884)
                     /locus_tag="VFX21_04875"
     CDS             complement(1444..1884)
                     /locus_tag="VFX21_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095320.1"
                     /translation="MTDRGRYAGKRVILSGAASGTGPATAGLLVDWGAEVHTIDATKP
                     KVRGLASFTECDLRAPEQVDAAVDRIGKYVHVLVNCAPHGPGVEHLIERVAADMLEGS
                     AIVCAGGTAAEAFVTGHARELAARGIDLTFADPPFVGPAGDRAD"
     gene            complement(1881..2075)
                     /locus_tag="VFX21_04880"
     CDS             complement(1881..2075)
                     /locus_tag="VFX21_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013830660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX5095321.1"
                     /translation="MKVRVDADLCVGHGRCYVLAPDVFTTDDYGHCVVIAETVDGALA
                     DQARVGADNCPERAIVVEDA"
     gene            2141..>3163
                     /locus_tag="VFX21_04885"
     CDS             2141..>3163
                     /locus_tag="VFX21_04885"
                     /EC_number="3.1.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF027980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ca2+-dependent phosphoinositide-specific
                     phospholipase C"
                     /protein_id="HEX5095322.1"
                     /translation="MPLQRLGIAVLVACSLVMAACSGDDDDNTSSSPDTEATTTTTFS
                     TVHRLDDTLRLNQMQVLASHNSYKGALYPQVLATLQSTVPEIAAGLEYSHRPLTEQFD
                     DFGIRAIELDAWADPQGGLYAQPAGPLSVGVTIPDDPAMRAPGFKVLHQSNVDTNSTC
                     LTLVACMELVRDWSDAHPGHAPMMVQIEVKDEAADAAEFDALDAEIRSVFDDDQLITP
                     DDVRGDAATLGEAVRSNGWPTLGETRGKVLFTLDNGGLRDVYLAGHPSLEGRVMFTPS
                     EPGADDAAFAKLNDPVGDKDAIAAALAANMIVRTRADADTVQARNNDTTDRDVALASG
                     AQFVSTD"
BASE COUNT          483 a         1098 c         1053 g          529 t
ORIGIN      
        1 gaggcacagg tcgagatatc cgaggccgag gccgcccgcc gactccggca gcgcgatgcc
       61 gagcaggttc gccttcgcga actcgctcca cgtgtcgcgg tcgtaccagt cctccgactt
      121 gtcgcgcgcc ttcaactgct gcaggtcggc gcggtcggcg aggatctgcg ccgcgagccc
      181 tcgcagctct tcttgttctt cggagaatgc aaagtccatg tctgctcccg tccggctcag
      241 cgcgggctgc cgggcatgtt gaggccgaag atcgcgatga ggtcgcgctg catctcgttc
      301 gtgccgccgc cgaacgtcag gatgtgcatg ctgcgcacga gacgttccac ccgaccgcgc
      361 aacaccgacc ccggagagtc gagcgacagc gtggccgcgt cgcccatgac ttccatgagc
      421 agccgcgcgg cctccatgta gaactcggtg ccgaacacct tgatgctcga cgcgtcggcg
      481 gggttgagcg gcgcgccggt ctgcgacagc caggcgacct tccagttcgc gaggcggagg
      541 aactcgagct tggcgtgcac gcgggccagg ttgacctgca cccactcctg gtcgatgacg
      601 cggcgcccgt cggcgagctt cgtctcctgg gcccaacggc ggacgtcgtc gaggttgcgt
      661 tcgatgatgc ccgacgagca cagcgtgacg cgctcgtggt tgagctggtt cgtgatgagg
      721 ttccagccgc cgttctcttc gccgacgaga ttggccgccg ggacgcgtac gtcgtcgtac
      781 gtcgtgatcg tcgtgttcag cccgccgaag ttctcgatcg gtaccgcgct gaacccgggt
      841 gtgtcggtcg gcacgatgat gatcgagatg cccttgtgct tcttgacctc ggtgttggtg
      901 cgcaccgcga gccacacgta gtcggaaccg ctcgcgaggc ttgtgaagat cttctgaccg
      961 ttgatcacgt actcgtcgcc gtcgcgcacg gcacgagtct tgagcgacgc gaggtcggtg
     1021 cccgcgtccg gctcggtgta accgatcgcg aagtggatct caccggcgag gatcttcggc
     1081 acatagaact gcttctgttc cgcgctgccg tagcgcatga tcgtcggcgc cacggagttg
     1141 atcgtcagca tcggtaccgg cgcaccggcg cgcatcgact cgtcgaagaa cacgaactgc
     1201 tcgatcggtg tgagtccgcg gccaccccac tccttcggcc agccgacgcc cgaccatccg
     1261 tcggacgcca tctgcttcca cgtcgccttc gacgcggggc cgatgccctc gcccttgcgg
     1321 atgtcgtctt cgatctcggg cgtgagcagc ttgtcgtagt aggcgcgaag ctcgtcgcgc
     1381 agcgcctctt gctccttcga caatccgacg tacatgcggc ctccggcgaa actagaacag
     1441 gtttcagtct gcccggtcgc cggcgggccc gacaaacggg gggtcggcga acgtcaggtc
     1501 gatcccgcgg gcggccagct cgcgtgcgtg gccggtgacg aacgcctcgg ccgcggtccc
     1561 gcccgcgcac acgatggccg agccttcgag catgtcggcc gcgacgcgtt cgatgaggtg
     1621 ctcgacaccc ggtccgtgcg gcgcgcagtt cacgagcacg tggacgtact tgccgatccg
     1681 gtcgaccgcg gcgtcgacct gctcgggggc gcgcaggtcg cactccgtga agctcgcgag
     1741 cccccgcacc ttgggcttcg tggcgtcgat ggtgtgcacc tcggcgcccc aatcgacgag
     1801 gagaccggcc gtcgccgggc cggtgccgga tgcggcgccg ctcaggatga ctctcttgcc
     1861 ggcgtaccga ccgcggtcgg tcacgcgtct tcgacgacga tggcgcgctc gggacagttg
     1921 tccgcgccga cgcgcgcctg gtcggccagt gcaccgtcga ccgtctcggc gatcacgacg
     1981 cagtgcccgt agtcgtcggt ggtgaacacg tcgggcgcca gtacgtagca acgtccgtgg
     2041 ccgacacaca gatcggcgtc cacacgcacc ttcacgtcca cgctccttcg gcgactgccc
     2101 gcagagcgta tcggagcccg gcgccctagc atccgcgttc gtgcccctgc agcgtctcgg
     2161 aattgccgtt ctcgtcgcgt gctcgctcgt catggccgct tgtagcggtg acgacgacga
     2221 caacacgtcg tcttcgcccg acaccgaggc gacgaccacg acgaccttct cgaccgtcca
     2281 ccgtctcgac gacacgttgc ggttgaacca gatgcaggtg ctggcgtcgc acaacagcta
     2341 caagggcgcg ctctacccgc aggtgctcgc gacgctgcag tccacagtgc ccgagatcgc
     2401 ggccggtctc gagtactcgc accgcccgct gacggagcag ttcgacgact tcgggatccg
     2461 cgcgatcgag ctcgacgcgt gggcggaccc gcagggcggc ttgtacgcgc agccggcagg
     2521 tcccttgtcg gtcggcgtca cgatccccga cgatcccgcg atgcgcgcac cgggcttcaa
     2581 ggtgctgcac cagtcgaacg tcgacaccaa ctcgacgtgc ctgacgctgg tcgcgtgcat
     2641 ggagctcgtg cgcgactggt ccgacgcgca cccgggccac gcgccgatga tggtgcagat
     2701 cgaagtgaag gacgaagcag ccgacgccgc ggagttcgat gcgctcgacg ccgagatccg
     2761 ctcggtgttc gacgacgacc agctcatcac gcccgacgac gtgcgcggtg acgcggcaac
     2821 gctcggcgaa gcggtgcgct cgaacggctg gccgacgctc ggcgagacgc gcggcaaggt
     2881 gctgttcacc ctcgacaacg gcggcttgcg cgacgtctac ctcgccggcc acccgtcgct
     2941 cgaagggcgc gtgatgttca cgccgtcgga gccgggcgcc gacgacgccg cgttcgcgaa
     3001 gctcaacgat cccgtcggtg acaaggacgc catcgcggcc gcgctcgccg cgaacatgat
     3061 cgtgcgcacg cgcgccgacg ccgacacggt gcaggcgcgc aacaacgaca cgaccgatcg
     3121 cgatgtggcg ctggcgagcg gtgcgcagtt cgtcagcacc gac
//
LOCUS       DASUGA010000247         2274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_516078, whole genome shotgun sequence.
ACCESSION   DASUGA010000247 DASUGA010000000
VERSION     DASUGA010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516078"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VFX21_04890"
     CDS             complement(<1..849)
                     /locus_tag="VFX21_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095323.1"
                     /translation="MRKFLSLLFVVIMAGAFFVACGGDDDDSTNASDDDTEQADSNDN
                     SGDDASNGDDASDDDSGDDDSTGSEELDKLLAQQADAHIKIVYDVSGDFASDGEGEEL
                     TVIQDGKDRSAWISGDTSYYSIDGKSISCSGLDSEPTCTEMPEGLGDLAALGPMTMLN
                     SIGQGLLQAGNDLDGLDKHDEEIAGRDAVCIDYDYGALLGGLADLGDLGDEDTPTDAK
                     VRICVDEETGYLLEFSGEGDGQKGGYVAKEVSEPEDSDFEPPADVEANPLGDLNLDEL
                     MPEDSDN"
     gene            complement(958..>2274)
                     /locus_tag="VFX21_04895"
     CDS             complement(958..>2274)
                     /locus_tag="VFX21_04895"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5095324.1"
                     /translation="DVLEVVGRNPLAEQATDRFSPLAEELAHKHPHRRDNTYPGGYEQ
                     VVQLFDSPAAPDLCVIHSASHNWEDQGGHLGEHGSPGVVQARAPLVIGGKGVRNLGLI
                     PRAARLTDIAPTFAAMLGCAPRDDGTYFAVQDGDALVDVLDPAERPDTVVGFLFDGTN
                     ANVLYDMVARGEAPNIARLLDMGVAFGHGAMASLPTVTLANHTTIITGAHPGHHGILH
                     NAWYDRARGEQIITNSSATWPTAMQHLSPGIESIHDVVHRTWPDAFTVSVNEPCDTGA
                     DYSTFEFFRRGEVPPIPRSPDGLPHTTERFVRPSKDYSWSSIVDHMGIDQATNIIAGS
                     YREVAYPRPKFMWCNFTLTDAAMHEGGPHSQMAAAAVRDSDGRVGEVLAALERAGTFD
                     DCAFLLVADHGMEESDPGCRGDWDVTLREHGLHFRDEGYGFLYFGV"
BASE COUNT          323 a          748 c          801 g          402 t
ORIGIN      
        1 gttgtcggag tcttcgggca tgagctcgtc gaggttcagg tcgccgagcg ggttcgcctc
       61 gacgtcggcc ggcggctcga agtcggagtc ctcgggctcg ctgacttcct tggcaacgta
      121 gccacccttt tggccgtcac cttcgccgga gaactcgagc aggtagccgg tctcctcgtc
      181 gacgcagata cggaccttgg catcagtggg cgtgtcttcg tcgccgaggt cgccgaggtc
      241 ggcgaggccg ccgagcaacg cgccgtagtc gtagtcgatg cacaccgcgt cgcggcccgc
      301 gatctcctcg tcgtgcttgt cgaggccgtc gaggtcgttg cccgcttgca gcaggccctg
      361 gccgatcgag ttgagcatcg tcatcggacc gagcgccgcg aggtcgccga gaccttcggg
      421 catctcggtg cacgtcggct cggagtcgag gcccgagcac gagatcgact tgccgtcgat
      481 gctgtagtac gaggtgtcgc cgctgatcca cgccgagcgg tccttgccgt cctggatgac
      541 ggtgagctct tcgccttcgc cgtcggacgc gaagtcaccg ctgacgtcgt agacgatctt
      601 gatgtgcgca tccgcctgct gcgcgagcag cttgtcgagc tcttcgctgc cggtcgagtc
      661 gtcgtcgccg gagtcgtcgt cactggcatc gtcgccgttg ctcgcgtcgt cgccgctgtt
      721 gtcgttgctg tcggcctgtt cggtgtcgtc gtcggaggcg ttggtgctgt cgtcgtcgtc
      781 accgccgcac gccacgaaga acgcgcccgc catgatcacg acgaacaaca gggagaggaa
      841 cttgcgcatg aatgcgtctc ctgcagtctt gggaaaatgc cgcgcccgcc tgaccggcag
      901 ggttcgcgca gcactcactc ggccgagatc cgctcggtct gagcgatcgt aggcccgtta
      961 aaccccgaaa tagaggaatc cgtacccttc gtcgcggaag tgcaggccgt gctcgcgcag
     1021 ggtgacgtcc cagtcgcccc ggcagcccgg gtcgctctct tccatgccgt ggtcggcgac
     1081 gagcaggaac gcgcagtcgt cgaaggtgcc ggcccgctcg agcgccgcga gcacttcgcc
     1141 cacgcgcccg tcggagtctc ggaccgcggc ggccgccatc tgggagtgcg ggccgccctc
     1201 gtgcatggcc gcgtcggtga gcgtgaagtt gcaccacatg aacttcgggc gcgggtaggc
     1261 gacctcgcgg tacgagccgg cgatgatgtt cgtcgcctgg tcgatgccca tgtggtcgac
     1321 gatcgacgac caggagtagt ccttcgaggg ccgcacgaac cgctcggtcg tgtgcggcaa
     1381 cccgtcgggc gacctgggga tgggcggcac ctcgccgcgc cggaagaatt cgaacgtcga
     1441 gtagtcggca ccggtgtcgc acggctcgtt caccgacacg gtgaacgcgt cgggccacgt
     1501 gcggtgcacg acgtcgtgga tggactcgat gccgggcgag agatgctgca tggccgtcgg
     1561 ccatgtcgcg ctcgagttcg tgatgatctg ctcaccgcgc gcccggtcgt accacgcgtt
     1621 gtggaggatg ccgtgatggc cgggatgcgc gccggtgatg atcgtggtgt ggttcgcgag
     1681 cgtgaccgtc ggcaacgacg ccatcgcgcc gtgtccgaac gccacaccca tgtcgagcag
     1741 ccgcgcgatg ttgggcgcct cgccgcgcgc caccatgtcg tacagcacgt tggcgttggt
     1801 gccgtcgaac aagaagccga cgaccgtgtc cggacgctcc gccgggtcga gcacgtcgac
     1861 gagcgcgtca ccgtcctgca cggcgaaata ggtgccgtcg tctcgcggcg cgcagccgag
     1921 catcgctgcg aacgtgggcg cgatgtcggt gagccgcgcc gcgcgcggga tcaggccgag
     1981 gttgcgcacg cccttgccgc cgatcaccaa tggtgcccgc gcctgcacca cgcccggcga
     2041 cccgtgctcg ccgaggtggc cgccttggtc ctcccagttg tgcgacgcgg agtggatgac
     2101 gcacaggtcg ggcgcggcgg gcgagtcgaa cagctggacg acctgctcgt agccaccggg
     2161 ataggtgttg tcacggcggt gcgggtgctt gtgggccaac tcctcggcca gcggcgagaa
     2221 ccggtcggtg gcctgctcgg cgagcgggtt ccgacccacg acctcgagga cgtc
//
LOCUS       DASUGA010000248         2005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1348165, whole genome shotgun sequence.
ACCESSION   DASUGA010000248 DASUGA010000000
VERSION     DASUGA010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348165"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /gene="fdhD"
                     /locus_tag="VFX21_04900"
     CDS             <1..603
                     /gene="fdhD"
                     /locus_tag="VFX21_04900"
                     /inference="COORDINATES: protein motif:HMM:NF014671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX5095325.1"
                     /translation="DIDTIAYCLGGEGEQLYNVVTLRTRTPVASAIRERRFLSNSSCG
                     ICGKAALEEVEVRCDPVGAGPVVAATVLGTLPAQLQDQQRVFGETGGLHAAARFTPDG
                     RLVSVREDVGRHNALDKLVGEALLARALPLANDVLLVSGRLSFELVQKAAVAGLPILC
                     AVSAPSSLAVAAAEQFGQTVVGFLRDGRFNVYSRPERVEL"
     gene            603..>2005
                     /locus_tag="VFX21_04905"
     CDS             603..>2005
                     /locus_tag="VFX21_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5095326.1"
                     /translation="MARPHRKHLIRSGGRMRRDLWVGLKPNGMGEQKPNHYGAMARTV
                     WENRKNLPWAWRILRKGVCDGCALGVAGFHDWTISGVHLCTTRLELLKVNTAAAIDDA
                     ALADVSALQRKNGKQLRELGRLAHPMVREKGDRGFRRVTWDEALDLVASRIEATTPDR
                     VGLYMTARGITNEVYYVAQKAMRAMGTNNVDNAARVCHAPSTAALKESVGVAATTCSY
                     RDVIESDLIVLFGADVANAQPVFMKYLYLAKKRGAKIAVVNPMREPGLERYWVPSNVE
                     SALFGTKLTDEFFAIHTGGDVAFVNGVMKQLLAIGGVDRRFITEHTNGFDALLAELED
                     EPFDMLERESGATRADMTRFAQMYANAKSAVLVWSMGVTQHAWGVDNVRAVVNLGLAR
                     GNVGRAGTGLMPIRGHSGVQGGAEMGCYATTFPGGVAINGDSAADLAAQWGFDVPAQP
                     GLTAGDMVDAARRGELDV"
BASE COUNT          328 a          662 c          689 g          326 t
ORIGIN      
        1 gacatcgaca cgatcgcgta ctgcctgggc ggtgagggcg aacagcttta caacgtcgtc
       61 acgttgcgga cgcgcacgcc ggttgcttca gccattcgcg aacgccgctt cctctcgaat
      121 tcgtcgtgcg gtatctgcgg caaggccgcg ctcgaggagg tcgaggtccg ctgcgacccg
      181 gttggcgccg gaccggtcgt cgcggcgacg gtcttgggca cgctgcccgc gcagctccag
      241 gaccaacagc gcgtgttcgg cgaaaccggc ggactgcatg ccgccgcccg attcacgccc
      301 gacggcaggc tggtgtcggt acgggaagac gtcggccgcc acaacgcgct cgacaagctc
      361 gtcggcgaag cgttgctggc gcgagcgttg ccgctcgcga acgacgtgtt actcgtctcg
      421 ggccgcctgt cgttcgaact cgtccagaag gcagccgttg cgggcctgcc gatcttgtgt
      481 gcggtgagcg cgccgtcgag tctggcagtt gccgccgccg aacagttcgg gcaaaccgtc
      541 gtcggctttc ttcgcgacgg ccggttcaac gtgtactccc gtcccgagcg cgtcgagctc
      601 tgatggcgcg tccgcatcgc aagcacctca tccgcagcgg cgggcgcatg cggcgcgacc
      661 tgtgggtcgg gctcaagccc aacggcatgg gcgagcagaa gcccaaccac tacggcgcga
      721 tggcgcgcac cgtgtgggag aaccgcaaga acctgccgtg ggcgtggcgc attctgcgca
      781 aaggcgtgtg cgacggctgc gcgctgggcg tcgccggatt ccacgattgg acgatcagcg
      841 gcgtgcacct ctgcaccacg cgcctcgagt tgttgaaggt caatacggcc gccgccatcg
      901 acgacgccgc gctcgccgac gtctccgccc tccaacgcaa gaacggcaag cagttgcgtg
      961 aactcggccg ccttgcgcat ccgatggtgc gcgagaaggg cgaccgcggt ttccggcgcg
     1021 tcacttggga cgaagcactc gacctcgtcg ccagccgcat cgaggcgacc acccccgatc
     1081 gcgtcggtct ctacatgacg gcgcgcggca tcaccaacga ggtgtactac gtcgcccaga
     1141 aggccatgcg ggccatgggc acgaacaatg tcgacaacgc cgcgcgcgtg tgtcacgcac
     1201 cgtcgaccgc cgccttgaag gagtcggtcg gcgtcgccgc gacaacgtgt tcgtacaggg
     1261 acgtcatcga gagcgatctc atcgtcctgt tcggagccga cgtcgcgaac gcgcagcccg
     1321 tgttcatgaa gtacctgtac ctcgcgaaga agcggggcgc gaagatcgcc gtcgtcaacc
     1381 cgatgcgcga gcccgggctc gagcgttact gggtgccctc gaatgtcgag agtgcgctgt
     1441 tcggcacgaa gctcaccgac gaattcttcg cgattcatac cggcggcgac gttgcgttcg
     1501 tcaacggtgt catgaagcag ctgctcgcga tcggcggcgt cgaccgcagg ttcatcaccg
     1561 agcacaccaa cggattcgac gcgctgttgg cggaactcga agacgaaccg ttcgacatgc
     1621 tcgagcgcga atcgggcgcg acccgggccg acatgacgcg gttcgcgcag atgtacgcga
     1681 acgcgaaatc agccgtgctc gtgtggtcga tgggtgtgac gcagcacgcg tggggcgttg
     1741 acaacgtgcg cgccgtcgtc aacctcggtc tcgcgcgcgg caacgtcggg cgcgcgggca
     1801 ccggactcat gccgattcgg ggccactccg gtgtgcaggg cggcgcggag atgggttgct
     1861 acgcgacgac gtttcccggc ggcgtggcga tcaatgggga ctcggcggcc gacttggctg
     1921 cgcagtgggg cttcgacgtt ccggcccagc ccggtctgac tgccggcgac atggtcgacg
     1981 ccgcgcggcg cggcgaactc gacgt
//
LOCUS       DASUGA010000249         4635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2010977, whole genome shotgun sequence.
ACCESSION   DASUGA010000249 DASUGA010000000
VERSION     DASUGA010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2010977"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /locus_tag="VFX21_04910"
     CDS             <1..1434
                     /locus_tag="VFX21_04910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5095327.1"
                     /translation="DAVANARRHMTFRRALLLIVALAFVVRVGYVVGAKSETCKIVVA
                     GEVRGSFGSECLAGDQYFYSAEANSLARGDGFVEPFAAILAPGQTPPAADHPPLTVVV
                     LAPVSWLADHTPLGDDADANVAAQRYAMVLLGTLLVFLVGLLGRRVGGDTVGLVAAAI
                     AALSPNIWVNDGLVMSETLTMVLTVAVLLAAFAWYDAPTARKGVLLGALFALAGLTRV
                     ELLLLVPLVMAPAVVRRAWAPRDRALRIATSLGVCALVLAPWVVFNLSRFDEPTFIST
                     NDGLTLAGANCDSTYHGEGTGLWVGRGVARCYGDVPSGLDQSEVSQRLRDIGLDYISA
                     HKSRAVVVAAVRVGRTWSLFHPADMIDFNKGEGRESWVTRLGLLVYYPTLVAAIGGAV
                     VLVRRRRFLLLWMVLVPAFVVTIASAVTYGQTRFRVPAEPSLAVLAAVAIVAAWDAFR
                     RRQVAASTGEPSSSSASETVTSAATGN"
     gene            complement(1356..3785)
                     /locus_tag="VFX21_04915"
     CDS             complement(1356..3785)
                     /locus_tag="VFX21_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095328.1"
                     /translation="MAPWLAILLVSMAGLLLEVSYTRIVSFKLWYYYTYLVIGLALLG
                     IGSGAVVTAVWPAMRRWSANSIVAVSSTIAALTTPLGFFVVARARVNTIAIWDYGSGS
                     SFTNLARLFLICFVLFATFVAMGVIVSVLLGRAGERVGRIYFADLVGAGIGCLVAVPL
                     IWWLEPPSVIALSSLTFAVVAVSTAAGAALRPVRIVSAIACIALAVVVVAGDVLPSVR
                     PEDGKLQVPTTLSTQWGPVFRVDVVAVPNVDDNRLLIHDGTYGSGIWEFDGDVSTLGR
                     YQTDTRAIPFLVRGGAPPKELIIGSAGGQEILASLHFKAPHIEAVELNPVTVGLLRDK
                     FAGFTGHLTDRDDVDMHVGDGRSYLARQDGDYDLIWYVAPDSYAATNAASSGAFVLSE
                     SYLYTTEMIKETLEHLDDDGIMVVQFGELDFEHSPNRTSRYVVTARTALEQLGIDDAP
                     DHLLVAASLTAGPDLSTILVKRTPFTDAEIANFRTGIATVPDTTEIHSPGTAAGESIV
                     SRLASAPDGEVDSIVADAPKDISAISDDAPYFWHFSPFPSVLRHIFEPLDVQDPEDVI
                     GERVLLLLLGIAAVYAAVFLLAPFVLVRRQWRALPKKGASAIYFAALGLGFMFFEITM
                     IQRLTLLLGYPTYSLTVTLAAILVFSGLGSLVSSRFAGRGRHTEFVLLGALAVLTAFY
                     QLALDDITDAVLDQSLAVRIVVTLLLLAPLGLCLGMFMPLGLGRVRTLSADGEQYVAW
                     SWAVNGFFSVIGSVLTTILSMTFGFQTVQVAGLAVYAIAVLAFTQLPVAADVTVSLAE
                     EDDGSPVLAAT"
     gene            3885..>4635
                     /locus_tag="VFX21_04920"
     CDS             3885..>4635
                     /locus_tag="VFX21_04920"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX5095329.1"
                     /translation="MTSLAAIEADDGTRLRASGYVPAVDGLRALAVAAVVIFHFSRDS
                     LRAGFLGVDVFFVVSGFLITRLLLREIEGTGSIGLVSFWGRRARRLLPALAVMTVIVV
                     IVSAIEFSDTEMHDIRAHALGSLFYVANWVFIHGDASYFATLGRPSPFLHTWTLAIEE
                     QFYLLFPLVMLVFRRGAVRRPLVAAGVAVGLAAASAVWMAHLVEPHRDPSRAYFGSDA
                     HASGILIGVALGLLFAAGAVDRVRAHRARAAN"
BASE COUNT          716 a         1509 c         1670 g          740 t
ORIGIN      
        1 gacgcggttg cgaacgcgcg ccgccacatg accttccggc gcgcgctgct cctgatcgtc
       61 gcgctcgcat tcgtcgttcg cgtcggatac gtggtcggcg ccaagagtga gacttgcaag
      121 atcgtggtcg cgggcgaggt gcggggatcc ttcggaagcg aatgcctggc cggggaccag
      181 tacttctaca gcgcggaagc gaactcgttg gcccgcggtg acgggttcgt cgaaccgttc
      241 gctgccatct tggcgccggg acagacgccg cccgcggcgg accatccacc gctgaccgtg
      301 gtggtgctcg caccggtgtc gtggctcgcc gaccacacgc cgctgggcga cgacgcagac
      361 gccaacgtcg ccgcgcagcg gtacgcgatg gtgctgctcg gcacgctgct cgtgttcctc
      421 gtcggcctgc tcggtcgacg cgtcgggggc gacaccgtcg gactcgtcgc ggccgcgatc
      481 gcggcgttgt caccgaacat ctgggtcaac gacgggctcg tcatgtccga gacgctcacg
      541 atggtgctga cggtcgccgt cttgctcgcg gcgttcgcgt ggtacgacgc gccaacggcc
      601 cgcaaggggg ttctcctcgg tgcgttgttc gccctggccg gtttgacacg cgtcgagctg
      661 ctgctgctcg tcccgctggt gatggcgccg gccgtcgtgc ggcgcgcgtg ggcgccacgc
      721 gaccgggcgc tgcgcatcgc aacgagtctc ggcgtgtgcg cgctcgtcct cgcaccatgg
      781 gtcgtcttca acctgtcgcg cttcgacgaa cccacgttca tctcgaccaa cgacggcttg
      841 acgctggccg gcgcgaactg cgacagcacg taccacggtg aggggaccgg gctatgggtc
      901 ggccgcggtg tggcgcgctg ctacggcgat gtgccgtccg gtctcgatca gagcgaagtg
      961 tcgcaacggc tccgtgacat cgggctcgac tacatcagtg cgcacaagtc gcgcgcagtc
     1021 gtggtggcgg ccgtgcgtgt tggtcgtacc tggagcctct tccatccggc cgacatgatc
     1081 gacttcaaca aaggagaggg ccgggagtcg tgggtgacgc ggcttggact gctcgtgtac
     1141 tacccgacgc tcgtcgccgc gatcggcggc gccgtcgtgc tcgtgcgccg gcggcggttc
     1201 ctgctcctgt ggatggttct cgtgcccgcg ttcgtcgtca cgattgcgtc ggcggtcacg
     1261 tacggacaga cgcgctttcg ggttccggcc gaaccgtcgc tcgcggtcct cgcggctgtc
     1321 gcgatcgttg ccgcgtggga cgcgttccgg cgccgtcagg tcgcggcgag cacgggcgag
     1381 ccgtcgtctt cttcggcgag cgagaccgtc acgtcggctg ctaccggcaa ctgagtgaac
     1441 gcgaggaccg cgatcgcgta gacggcgaga cccgcgacct gcacggtctg gaagccgaac
     1501 gtcatcgaca ggatggtcgt cagcaccgag ccgatgaccg agaagaagcc gttcaccgcc
     1561 cacgaccacg cgacgtattg ctcgccgtcg gcgctgagcg tgcggacgcg gccgaggccg
     1621 agcggcatga acatgccgag gcagaggccg agcggggcca gcaacaacag cgtgacgacg
     1681 atgcggaccg cgagactctg gtcgagcacc gcatcggtga tgtcgtcgag cgcgagttgg
     1741 tagaacgccg tcagcaccgc gagcgcgccg agcagcacga actctgtgtg gcgaccgcgg
     1801 cccgcgaagc gcgagctcac cagcgacccg agtcccgaga acacgaggat cgcggccagc
     1861 gtcaccgtga gcgaataggt ggggtatccg agcagcaagg tcaaccgctg gatcatcgtg
     1921 atctcgaaga acatgaagcc gagcccgagc gcggcgaagt agatcgcgct ggcgcccttc
     1981 ttcggcaggg cgcgccactg gcggcggacg agcacgaacg gcgcgagcag gaagaccgcg
     2041 gcgtagacgg ctgcgatgcc gagcaggagc agcagcacac gttcaccgat cacgtcctcg
     2101 gggtcttgca cgtcgagcgg ctcgaagata tgacgcaaca ccgatgggaa cggcgagaag
     2161 tgccagaagt acggcgcgtc gtcggagatc gcgctgatgt ccttgggcgc gtcggcgacg
     2221 atcgagtcga cctcgccgtc gggggcgctc gcgaggcgcg acacgatgct ctcgccggca
     2281 gcggtgccgg gggagtggat ctcggtcgtg tcgggcacgg ttgcgatgcc ggtgcggaag
     2341 ttggcgatct ccgcgtcggt gaacggcgtg cgcttgacga ggatcgtcga cagatcgggc
     2401 ccggccgtca gcgacgccgc gacgagcagg tggtcgggcg cgtcgtcgat gcccagctgc
     2461 tcgagcgccg tgcgcgcggt gacgacgtag cggctcgtgc gattcggcga atgctcgaag
     2521 tcgagctcac cgaactgcac gaccatgatc ccgtcgtcgt cgagatgctc gagcgtctcc
     2581 ttgatcatct cggtcgtgta gaggtagctc tcggagagca cgaacgcgcc cgacgatgcc
     2641 gcgttcgtcg ccgcgtagct gtcgggcgcg acgtaccaga tgaggtcgta gtcgccgtcc
     2701 tggcgcgcga ggtacgaacg accgtcgccg acgtgcatgt cgacgtcgtc gcggtcggtc
     2761 agatgaccgg tgaagccggc gaacttgtcg cgcaacagcc cgacggtcac cgggttgagc
     2821 tcgacggcct cgatgtgcgg cgccttgaag tgcagcgacg cgaggatctc ttggccgccc
     2881 gcggagccga tgatcagctc cttgggtggc gcgccaccgc ggacgaggaa cgggatggcg
     2941 cgcgtgtcgg tctggtagcg cccgagcgtg gacacgtcgc cgtcgaactc ccagatgccc
     3001 gatccgtacg tgccgtcgtg gatcagcagc cggttgtcgt cgacgttcgg caccgcgacg
     3061 acgtcgaccc ggaagaccgg tccccactgg gtcgacagcg tcgtcggcac ctgcagcttg
     3121 ccgtcttcgg ggcggacgct cggcaggacg tcgccggcca cgaccaccac ggcgagagcg
     3181 atgcaggcga tcgcgctgac gatccgcacc ggacgcagcg ccgcgccggc cgcggtgctc
     3241 accgcgacga cggcgaaggt caacgacgac aacgcgatga cgctcggcgg ttcgagccac
     3301 cagatcaacg gcaccgcgac gaggcaaccg atgcccgcgc cgacgagatc cgcgaagtag
     3361 atgcggccga cgcgctcgcc ggcgcgcccg agcagcaccg agacgatgac gcccatcgcg
     3421 acgaaggtcg cgaacaacac gaagcagatc aggaacaggc gggcgaggtt ggtgaacgag
     3481 ctgccgctgc cgtagtccca gatcgcgatc gtgttgacgc gcgcgcgcgc gacgacgaag
     3541 aagccgagcg gcgtcgtgag cgcggcgatc gtcgacgaca ctgcgacgat cgagttggcg
     3601 gaccaccggc gcatggccgg ccacaccgcg gtgacgacgg caccggaccc gataccgagc
     3661 agtgcgagcc cgatgacgag gtaggtgtag tagtaccaga gcttgaacga gacgatgcgg
     3721 gtgtagctga cctcgaggag caagccggcc atgctgacga gcaagatcgc gagccacggc
     3781 gccattcggg ccgagtcgcg gcgcgtcgct ggcattctgc gcgtccccca agaggcagcc
     3841 cgccgtgggt cgtcgggcgc aggcacgata gcgcgggagt tgcgttgact tcgctcgcag
     3901 cgatcgaagc cgacgacggc acacgcctgc gtgcgagcgg ctacgtaccc gcggtggacg
     3961 gcctgcgcgc actcgcggtg gccgcggtcg tcatcttcca cttctcgcgc gattcgctgc
     4021 gtgcgggctt cctgggagtc gacgtgttct tcgtcgtctc cggcttcctg atcacgcgtc
     4081 tgctgctgcg cgagatcgag ggcacgggca gcatcgggct cgtgtcgttc tggggccggc
     4141 gggcgcggcg gctgttgccg gcgctggccg tgatgaccgt catcgtcgtg atcgtgagcg
     4201 cgatcgaatt cagcgacacc gagatgcacg acatccgcgc gcacgcgctc ggttcgttgt
     4261 tctacgtcgc caactgggtg ttcatccacg gggacgcgtc gtacttcgcg accctcgggc
     4321 gcccctcgcc gttcctgcac acgtggacgc tcgcgatcga agaacagttc tacctgctgt
     4381 tcccgctcgt catgctggtg ttccggcgtg gcgccgtgcg ccgaccgctg gtcgcggcgg
     4441 gtgtcgcggt cggtctggcg gcggcatccg cggtgtggat ggcccatctc gtggagccgc
     4501 accgcgatcc gtcgcgcgcg tacttcggaa gcgatgcgca cgcgtcggga atcctgatcg
     4561 gcgttgcgct cgggctgttg ttcgcggcgg gtgccgtcga tcgcgtgcgt gcgcaccggg
     4621 cgcgcgccgc gaacg
//
LOCUS       DASUGA010000250         3133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_245189, whole genome shotgun sequence.
ACCESSION   DASUGA010000250 DASUGA010000000
VERSION     DASUGA010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245189"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /locus_tag="VFX21_04925"
     CDS             <1..1240
                     /locus_tag="VFX21_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5095330.1"
                     /translation="GGSGWVDMIDIDPADLVDPERYAQRGYPHDVWTRLRAEAPVARI
                     KSPGYEPFWAITKHADILQIAAQPERFSSAQGITLAREGAAPVPPTEILVMIDPPKHG
                     PMRRVVNARFTPRAVRARQDDVERIAVDVVDGAAATGDVDFVARIAAPFPLAVIAWIL
                     GVPSDDWPHLFRWTNEIIGKDDPEYRQPGESPGKTMKRARGELRGYFERLVEECRAAP
                     RDGLVTELINGTINGEPLTHEQLISYCELFVEAGNETTRNAISGGLLAFCEHPGEWEK
                     LRANPDLLPSATEEILRWASPISHFTRVATEDCEIRGVPIAAGDQLALYFASANRDEE
                     VFDDPFAFRVDRTPNPHLAFGFGEHVCLGAHLARVEIETIYRHLVTRLESFELAGEVE
                     RLKSAVNGSIKQLPLRYRLV"
     gene            1264..2262
                     /locus_tag="VFX21_04930"
     CDS             1264..2262
                     /locus_tag="VFX21_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5095331.1"
                     /translation="MEARPFGRTGLDVGAVGYGCWEIGGGYGDIEEQDFVRAVGRALD
                     LGINCFDTAEGYGMGASERSLGQALGSRRDEAVVVTKFGMNYKDKPNLRDSSRERVIA
                     SIDKSLKNLGTDYVDVYLIHWPDRLTPFEETMSALDDIVREGKVRFVGVSNFKKEEIE
                     ACMAVRRVDVVQYGWNMFDRRMQTEILPYCAEHGIGFMAYGSLAFGLLTGTFTEDHDF
                     GAADWRARQGNMGAIKMFAALFGPEKFKDNVRAVEELKGIATKYEKSLPQLALRWALE
                     HPAVSTALVGCRNVAEVEDNAGATGWSISSDDLAAIDAIFAKYEIDTTPDFWIEEA"
     gene            2267..3040
                     /locus_tag="VFX21_04935"
     CDS             2267..3040
                     /locus_tag="VFX21_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007777473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5095332.1"
                     /translation="MSRELDGMVAIVTGGAGGLGRGMVECFAEEGASVVVADVNAEGG
                     EALASGLGDTVAFKQTDVSDADQIQDVVDFAVERFGGLHVMCNNAGLGGSFAPFLKDD
                     FADFDRSIAVNLYGVMIGCQRAARHMAGHGGGVIVNTTSIGGITAGAGVMVYRATKAA
                     VIHMTRSLAIELAPKDIRVNCIAPAHIPTAINENLDQSLIIKAMQPLQRQGSPRDVGN
                     AAVFLASDRAAQITGIVLPVDGGTTAGPPPRRLEDLKKQ"
BASE COUNT          544 a         1053 c         1070 g          466 t
ORIGIN      
        1 cggagggagc gggtgggttg acatgatcga catcgatccc gccgacctcg tcgacccgga
       61 gcggtacgcg cagcgcgggt acccgcacga cgtgtggacg cggctgcgcg cggaggcgcc
      121 ggtcgcgcgc atcaagtcgc ccggttacga gccgttctgg gccatcacca agcacgccga
      181 cattctccag atcgcggcgc agcccgaacg gttctccagt gcccagggca tcacgctcgc
      241 gcgggagggc gcggcgccgg tgccgccgac cgagatcctg gtgatgatcg acccgccgaa
      301 gcacgggccg atgcgccgcg tcgtcaatgc gcgcttcacg ccgcgcgcgg tacgagcacg
      361 acaagacgac gtcgaacgca tcgccgtcga cgtcgtcgac ggtgcggccg cgaccggcga
      421 cgtcgacttc gtggcgcgca tcgcggcgcc gttcccgctt gcggtgatcg cgtggatcct
      481 cggcgtgccg agcgacgact ggccgcacct cttccgctgg accaacgaga tcatcggcaa
      541 ggacgatccg gagtaccgcc aacccggtga gtcgccgggc aagacgatga agcgggcgcg
      601 cggtgagctg cgcggttact tcgaacggct cgtcgaggag tgccgggccg caccgcgcga
      661 cggcctcgtc accgagctga tcaacggcac gatcaacggc gagccgctca cgcacgaaca
      721 gctcatctcc tactgcgagt tgttcgtcga ggccggcaac gagacgaccc gcaatgcgat
      781 cagcggcggg ctcctcgcgt tctgcgagca cccgggcgaa tgggagaagc tgcgggcgaa
      841 tcccgacctg cttccgagcg cgaccgaaga gatcctgcgc tgggccagcc cgatcagcca
      901 cttcacacgg gtcgcaaccg aggactgtga gatccgcggt gtgccgatcg cggccggcga
      961 ccagctcgcg ttgtacttcg cgtccgcgaa ccgggacgag gaagtgttcg acgacccgtt
     1021 cgcgttccgg gtcgaccgca ccccgaaccc gcacctcgcg ttcggcttcg gtgagcacgt
     1081 gtgtctcggc gcgcacctgg cgcgcgtcga gatcgaaacg atctaccgcc accttgtcac
     1141 tcggctcgag tcgttcgagc tggcgggtga ggtcgagcgg ctgaaatccg ccgtcaacgg
     1201 cagcatcaaa caacttccgc tgcgctaccg cttggtgtag caagcacttc gagaggggac
     1261 acgatggaag cccgaccgtt cggacgcacc ggcctcgacg tcggcgccgt tggctacggc
     1321 tgttgggaga tcggcggcgg ctacggcgac atcgaagagc aagacttcgt gcgcgccgtc
     1381 ggccgcgcgc tcgacctcgg catcaactgc ttcgacacgg ccgagggcta cggcatgggc
     1441 gcgtccgagc gatcgctcgg gcaggcgctc ggcagccgtc gcgacgaggc agtcgtcgtc
     1501 accaagttcg gcatgaacta caaggacaag ccgaacctgc gcgacagcag ccgcgagcgt
     1561 gtcatcgcgt cgatcgacaa gagcctgaag aacctcggta ccgactacgt cgacgtgtac
     1621 ctcatccact ggcccgaccg gctcacgccg ttcgaggaga cgatgagcgc gctcgacgac
     1681 atcgtgcgcg agggcaaggt gcgcttcgtc ggcgtgtcga acttcaagaa ggaagagatc
     1741 gaagcgtgca tggcggtgcg gcgagtcgac gtcgtgcagt acggctggaa catgttcgac
     1801 cgccgcatgc agaccgagat cctcccgtac tgcgcggagc acggcatcgg cttcatggcc
     1861 tacgggtcgc tcgcgttcgg tctgctcacc ggcacgttca ccgaggacca cgacttcggc
     1921 gcggccgact ggcgggcgcg ccagggcaac atgggcgcga tcaagatgtt cgcggcgctc
     1981 ttcggccccg agaagttcaa ggacaacgtg cgcgcggtgg aggagctgaa gggcatcgcg
     2041 acgaagtacg agaagagcct cccgcagctc gcgttgcggt gggcgctcga gcaccccgcg
     2101 gtcagcactg cgctcgtcgg gtgccgcaac gtcgccgagg tcgaggacaa cgcgggtgcg
     2161 accggctggt cgatcagcag cgacgacctc gcggcgatcg acgccatctt cgccaagtac
     2221 gagatcgaca ccacgcccga cttctggatc gaagaggcgt agagagatgt cgcgcgagct
     2281 ggatggcatg gtcgcgatcg tgaccggcgg tgccggcggg ctcggtcgcg gcatggtcga
     2341 gtgcttcgcc gaagagggcg cgtcggtggt cgttgccgac gtgaacgccg aaggcggcga
     2401 agcgctcgcg agtggtttgg gtgacaccgt cgcgttcaaa cagaccgacg tctccgacgc
     2461 ggaccagatc caggacgtcg tcgacttcgc cgtcgaacgc ttcggcgggc tccacgtgat
     2521 gtgcaacaat gcggggctcg gcggctcgtt tgccccgttt ctgaaggacg acttcgccga
     2581 cttcgaccgt tccatcgcgg tcaacctcta cggcgtcatg atcggctgcc agcgcgcggc
     2641 ccggcacatg gccggccacg gcggcggcgt gatcgtcaac accacctcga tcggcggcat
     2701 caccgcgggc gcgggcgtca tggtctaccg agccaccaag gccgcggtga tccacatgac
     2761 gcgctccctc gcgatcgagt tggccccgaa ggacatccgc gtgaactgca tcgcgccggc
     2821 gcacatcccg actgcgatca acgagaacct cgaccagtcg ttgatcatca aggcgatgca
     2881 accgctgcaa cggcagggtt cgccgcgcga cgtcggcaac gcggcggtgt ttctcgcgag
     2941 cgaccgcgcc gcgcagatca ccggcatcgt cttgccggtc gacggcggga cgaccgcggg
     3001 gccgccgccc cgccggctcg aggacctgaa gaagcaatga ccgcgacgat ctactcctgc
     3061 gacgaccatc tcgacctcta tgcggtgccg cgcgacgtct ggaccacgcg cctctcggcc
     3121 gcggacgcgg cac
//
LOCUS       DASUGA010000251         4149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_131503, whole genome shotgun sequence.
ACCESSION   DASUGA010000251 DASUGA010000000
VERSION     DASUGA010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131503"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VFX21_04940"
     CDS             complement(<1..385)
                     /locus_tag="VFX21_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5095333.1"
                     /translation="MARRDAFIDHLQQVTLFSACSRKDLQLVARRAEDVKVPAGKVLV
                     AEGETGHEFFVILEGNAKVTRQGRKIADIGPGAAFGELALLDKAPRNATVVAESDMEL
                     VVLGQREFAGIIDEVPGFSRKLLAGM"
     gene            548..2392
                     /gene="dnaK"
                     /locus_tag="VFX21_04945"
     CDS             548..2392
                     /gene="dnaK"
                     /locus_tag="VFX21_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX5095334.1"
                     /translation="MSKAVGIDLGTTNSVVSVLEAGEPVVIPNAEGSRTTPSVVAFSK
                     TGEVLVGEVAKRQAITNPDRTIRSVKRHMGTNWSVDIDGKAYKAQEISARTLMKLKRD
                     AEQYLGTSVTQAVITVPAYFDDAQRQATKEAGEIAGLEVLRIINEPTAAALAYGLDKE
                     EHDQTILVFDLGGGTFDVSVLDIGDGVFEVKSTAGNTHLGGDDWDQRVIDWMVTEFKN
                     AHGVDLSVDKMAIQRLKEAAEKAKIELSNLQETSINLPFITATADGPLHLELSLTRAR
                     FQEMTADLLNACRGPFEQAIKDAKLTKDQIQHVILVGGSTRMPAVSDLVRELTGKEAH
                     KGVNPDEAVAIGASIQAGVLKGEVKDILLLDVTPLTLGIMVKGELMEPLIERNSTIPT
                     KKSKTFTTADDNQPSVEIKVLQGESQVATRNKPLGTFTLAGIPPAPRNVPQIEVTFDI
                     DANGIVHVSAKDLGTGKEQSMTITGGTALNKDEIDRMVKDAEAHAEEDKRKVEEAEVR
                     NGADSLAYQTETLLREQGDKVTPDDKTKIEESLKALKDALAGTDIEAVKLAHENLLSA
                     SNDFSRRLYESVSAQQQAAGAGTAGPSDAPSDDEVADAEIVDDDQQSA"
     gene            2399..2941
                     /gene="grpE"
                     /locus_tag="VFX21_04950"
     CDS             2399..2941
                     /gene="grpE"
                     /locus_tag="VFX21_04950"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX5095335.1"
                     /translation="MTDHNESAPSLDDDVVDGSAADDVQEIEADLMQLTQLQSERDEL
                     LDATRRLQADFENYRKRVLREQTSIVERAAEGLIEQLLPVLDSFELAVRNLEGDDVDI
                     EKVRKGVELVYAELFATLEKAGLERVQALGEPFDPNEHEAVMQDDGDADPVVGEVFRP
                     GWKLKGRVMRPAMVKVTRNP"
     gene            2942..4129
                     /gene="dnaJ"
                     /locus_tag="VFX21_04955"
     CDS             2942..4129
                     /gene="dnaJ"
                     /locus_tag="VFX21_04955"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018547806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX5095336.1"
                     /translation="MAAEREWFDKDYYAVLGVPMSAPDKDVARAYKKLAKQFHPDANP
                     GNKDAEERFKEISAAYDVLGDANKRKEYDEVRRMVASGVGPGFVGGGPGGQSFTFDTS
                     GADSGIFSDLLGGFFGGGRGRGGGRRAGQTASQRGSDLETDLQIGFDDAVHGVTSTVR
                     FRADASCSTCNGTGAEPGTHPETCPECHGTGQVAVEQGPFSFAQVCPTCSGRGQVVAH
                     PCPTCRGRGVENKAREVKVRIPAGVADGQRIRVKGRGAAGVRGGQPGDLYVNVRVGKH
                     PVFGRDGDNVTVKVPVTFAEATLGADVRVPTLDGQVTVRIPPGTPSGKVLRVRGKGVA
                     GNNGKGGGDLLVTVDVTVPTELNDEQREALDSLAKAFDADVRAPLFTQQHNRRRTDAD
                     GTA"
BASE COUNT          787 a         1326 c         1442 g          594 t
ORIGIN      
        1 ccatacccgc gaggagcttg cggctgaagc ccggcacttc gtcgatgatg ccagcgaact
       61 cgcgctggcc gaggaccacg agctccatgt cggactcggc gacgacggtc gcgttgcgcg
      121 gtgccttgtc gaggagggcg agctcgccga acgcggcgcc gggaccgatg tcggcgatct
      181 tgcggccctg gcgggtgacc ttggcgttcc cctccaaaat gacgaagaat tcgtggcccg
      241 tctcgccttc ggcgaccagg accttgcccg cggggacctt cacgtcctcg gcccggcggg
      301 cgaccagctg gagatctttg cgggagcagg cgctgaacag ggtgacctgt tgcaggtggt
      361 cgatgaaggc gtcacggcgt gccatagggc cggaatgtaa ctggcatcac gctcggcgtc
      421 acgttccccg agaaatacgg catccgcggg aatctgatac gaccgcgctc aagttatact
      481 cggcacacct actcaggttt cccgcgtgga aacccttcat gttcagtttt gtccagggag
      541 gaccagtatg tccaaggccg tcgggatcga tctcggcacc acgaactcag tcgtctcggt
      601 gctggaggcc ggcgaacccg tcgtcatccc caacgccgag ggctcgcgca cgacgccttc
      661 ggtcgttgcg ttctcgaaga ccggcgaggt cctcgtcggt gaggtcgcca agcgccaggc
      721 gatcaccaac cccgaccgca cgatccgctc cgtgaagcgc cacatgggca cgaactggag
      781 cgtcgacatc gacggcaagg cgtacaaggc gcaggagatc tccgcgcgca cgctgatgaa
      841 gctcaagcgc gacgcggagc agtacctcgg cacgagcgtg acccaggcgg tcatcaccgt
      901 gcccgcgtac ttcgacgacg cgcagcgcca ggccaccaag gaagccggtg agatcgcggg
      961 cctcgaagtg ctgcgcatca tcaacgagcc caccgcggcc gcgctcgcgt acgggctcga
     1021 caaggaagag cacgaccaga cgatcctcgt gttcgacctc ggcggcggca ccttcgacgt
     1081 gtcggtgctc gacatcggcg acggtgtctt cgaggtgaag tcgaccgcgg gcaacacgca
     1141 cctcggcggt gacgactggg accagcgcgt catcgactgg atggtgacgg agttcaagaa
     1201 cgcgcacggc gtcgacctgt ccgtcgacaa gatggcgatc cagcggctga aggaagccgc
     1261 ggagaaggcg aagatcgagc tctccaactt gcaggagacg tcgatcaact tgccgttcat
     1321 caccgcgacc gcggacggcc cgctgcacct ggagctgtcg ctcacgcgcg cgcgattcca
     1381 ggagatgacc gcggacctgt tgaacgcgtg ccgcggcccg ttcgagcagg cgatcaagga
     1441 cgccaagctc accaaggacc agatccagca cgtcatcctc gtcggtggtt cgacgcgtat
     1501 gccggcggtc agcgacttgg tgcgtgagct cacgggcaag gaagcgcaca agggcgtcaa
     1561 ccccgacgaa gcggtggcga tcggtgcgtc gatccaggcc ggcgtgttga agggcgaggt
     1621 caaggacatc ttgctcctcg acgtcacgcc gctgacgctc ggcatcatgg tcaagggtga
     1681 gttgatggag ccgctcatcg agcgcaacag caccatcccg accaagaagt cgaagacgtt
     1741 cacgaccgcc gacgacaacc agccgagcgt cgagatcaag gtgctccagg gtgagtcgca
     1801 ggtcgcgacg cgcaacaagc cgctgggcac gttcacgctc gcgggtatcc cgcccgcacc
     1861 acgcaacgtg ccgcagatcg aggtcacctt cgacatcgac gcgaatggca tcgtgcacgt
     1921 gtccgcgaag gatctgggca cgggcaagga gcagtcgatg acgatcaccg gcggcaccgc
     1981 gctcaacaag gacgagatcg accgcatggt caaggacgcc gaggcgcacg ccgaggaaga
     2041 caagcgcaag gtcgaggagg ccgaggttcg caacggcgcg gactcgctcg cctaccagac
     2101 cgagacgttg ctgcgtgagc agggcgacaa ggtcacgccc gacgacaaga ccaagatcga
     2161 ggagtcgttg aaggcgttga aggacgcgct cgccggcacc gacatcgagg ctgtgaagtt
     2221 ggcgcacgag aacctgctca gcgcgagcaa cgacttctcg cggcggttgt acgagtcggt
     2281 ttctgcgcag cagcaggctg ccggtgccgg caccgcgggt ccctcggacg ccccctcgga
     2341 cgacgaggtc gcggacgccg agatcgtcga cgacgaccag cagagcgcat aacccgacat
     2401 gactgaccac aacgagtcgg caccgagcct cgacgacgac gtcgtcgacg gctcggcggc
     2461 cgacgacgtc caagagatcg aagccgacct gatgcagttg acgcagttgc aatccgagcg
     2521 tgacgagttg ctcgacgcga cgcgtcgtct gcaggccgac ttcgagaact accgcaagcg
     2581 cgtcctgcgc gagcagacct cgatcgtcga gcgcgcggcc gaagggctca tcgagcagct
     2641 gctaccggtg ctcgacagct tcgagctcgc ggtccgcaac ctcgaaggtg acgacgtcga
     2701 catcgagaag gtccgcaagg gtgtcgagct cgtctatgcg gagctgttcg ccacgctcga
     2761 gaaggcgggg ctcgaacgag tgcaggcgct cggcgagccg ttcgacccga acgagcacga
     2821 agcagtgatg caagacgacg gcgatgccga tcccgtggtc ggcgaagtct tccgtcccgg
     2881 ttggaagctg aagggtcgtg tgatgcggcc cgcgatggtg aaagtgacga ggaaccccta
     2941 gatggccgcg gagcgcgagt ggttcgacaa ggactactac gccgtgttgg gtgtgccgat
     3001 gagcgccccc gacaaggacg tcgcgcgcgc gtacaagaag ctggccaagc agtttcaccc
     3061 cgacgccaac cccggcaaca aggatgccga ggagcgcttc aaggagatct ccgccgccta
     3121 cgacgtgctc ggtgacgcga acaagcgcaa ggagtacgac gaagtccggc gcatggtggc
     3181 gagtggtgtc ggcccggggt tcgtcggcgg tggacccggt ggccagtcgt tcaccttcga
     3241 cacgtcgggc gccgacagcg gcatcttctc cgacttgctc ggcggcttct tcggtggcgg
     3301 gcgcggtcgc ggcggcggtc ggcgcgcggg acagaccgcg tcacagcgcg gcagcgacct
     3361 ggagaccgat ctgcagatcg ggttcgacga cgcggtgcac ggtgtgacgt cgacagtgcg
     3421 cttccgggcc gacgcgtcgt gctcgacgtg caacggcacc ggcgcggagc cgggcacgca
     3481 ccccgagacg tgtcccgagt gccacggcac gggacaggtc gcggtcgagc aagggccgtt
     3541 ctcgttcgcg caggtgtgcc cgacgtgctc cggccgcggg caggtggtcg cgcacccgtg
     3601 cccgacgtgc cgcgggcgcg gcgtcgagaa caaggcgcgc gaggtcaagg tccgcattcc
     3661 ggcgggtgtt gccgacggcc agcgcatccg cgtgaaggga cgcggcgcgg cgggcgtgcg
     3721 cggcggtcag cccggcgacc tctacgtcaa cgtgcgcgtc ggcaagcacc cggtcttcgg
     3781 gcgcgacggc gacaacgtga cggtgaaggt gccggtgacg ttcgcggagg cgacgctcgg
     3841 tgccgatgtg cgggtgccga cgctcgacgg gcaagtgacg gtgcgcatcc cgcccgggac
     3901 acccagcggg aaggtgctgc gcgtgcgcgg caagggcgtg gccggcaaca acggcaaggg
     3961 tggcggcgat ctgctcgtca ccgtcgacgt gaccgtgccg accgagctca acgacgaaca
     4021 acgagaagcg ctggattcgc tcgcgaaggc gttcgacgcc gatgtgcgcg cgccgctctt
     4081 cacacagcaa cacaatcgca ggaggacgga cgccgatgga accgcatgac gcacgcgcgt
     4141 tgtacgtca
//
LOCUS       DASUGA010000252         3290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_500712, whole genome shotgun sequence.
ACCESSION   DASUGA010000252 DASUGA010000000
VERSION     DASUGA010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500712"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFX21_04960"
     CDS             <1..353
                     /locus_tag="VFX21_04960"
                     /inference="COORDINATES: protein motif:HMM:NF015665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HEX5095337.1"
                     /translation="VLGLTFKANTDDLRESPAMTITKDLVSGGASVCAFDPVAGHAAA
                     NEIPGLEIATDAYEACDDAEVLAILTEWDQFRWLDYTRVVKAMAKPQVVDARNLLDPA
                     AMRRLGFTYQGVGR"
     gene            353..1291
                     /locus_tag="VFX21_04965"
     CDS             353..1291
                     /locus_tag="VFX21_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEX5095338.1"
                     /translation="MPRAVLTGGAGFLGSHLATELRRGGYDVVAVDDLSTGRVENLDH
                     LADDPGFTLLEHDVSRGIPIDGPVAAVLHFASPASPPAYLARPLETLAVGSEGTRHAL
                     ELARAHGARFLLASTSEIYGDPLVHPQPESYLGNVNSVGPRAVYDEAKRFAEAITMAY
                     HREYGLDTKIVRIFNTYGPRMAPGDGRVVTNFLEQALAGRPLTVYGDGSQTRSFCWVD
                     DEVRGFLALLASDWTGPMNVGNPGEFTVLELAKIVIEVTASDSEIVFEPLPVDDPTRR
                     RPDITLARDVLGWEPEVDLRTGVARLADWYRTQRMS"
     gene            1288..2172
                     /locus_tag="VFX21_04970"
     CDS             1288..2172
                     /locus_tag="VFX21_04970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5095339.1"
                     /translation="MNSRWAAVVVNYEAGDALVACVRSLLADDSAGVPPELVVVDNGS
                     RDGSVATLRRELPGITVIEPHANLGYAGAANRGIARTTAPIVAVCNADLVIAPGAGAV
                     MLAAFADDTVGAAGPCIRNLDGSVYPSVRRDDGVVDATGHAVLGSVWPTNPFTRRYRE
                     LDADPTVARDVEWASGAALWLRRDAIEGVGGWDEGFFMYLEDVDVGWRLRKAGWRVRY
                     EPAAEIVHIQGLSTDQHRTRMILEHHRSTYRFASKRWQGWQRLLLIPTAALLAVRAAF
                     TVAWDRLRPRRSRSDITR"
     gene            2184..2981
                     /locus_tag="VFX21_04975"
     CDS             2184..2981
                     /locus_tag="VFX21_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095340.1"
                     /translation="MPKSRNKYSRARMRERSRKSRHQRTSGWFYATVAVIVVIGSVTI
                     GAIVMGRDDNNTAPVIGDHWHAAFGVNICGEWISNPATFETPANNSGVRAGIHTHGDG
                     FIHIHPFARAETGNNATLGKFLSYGGWSASEDSISVWTGPDVDKSKTTWTNGDKCPSG
                     SEEGAGEPGRVVFEVNCKTVEGNPSDHKLADQEVVAIGFVPKGVEIGAPPNAASAPEN
                     DSGTGTTPEAINQKECRPSAENNPGVPETVPPATTATTTPSTTSPTP"
     gene            2978..>3290
                     /locus_tag="VFX21_04980"
     CDS             2978..>3290
                     /locus_tag="VFX21_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX5095341.1"
                     /translation="MKAVVLVGGEGTRLRPLTYATPKPLLPIANQPFLERQLTWMHRH
                     GIDEVVLSLGYLPDAFAQHFAHGSFGDVRLQYAVEDEPLGTAGAIRYAAEVGGIDERF
                     VV"
BASE COUNT          515 a         1176 c         1129 g          470 t
ORIGIN      
        1 cggtgctcgg gctcacgttc aaggccaaca ccgacgacct gcgcgagtcc ccggccatga
       61 cgatcaccaa ggacctcgtt tcgggcggtg cgtcggtctg cgcgttcgat cccgtggccg
      121 ggcacgcggc ggcgaacgag atccccggcc tcgagatcgc caccgacgcg tacgaggcgt
      181 gcgacgacgc cgaggtgctg gcgatcctca ccgagtggga ccagttccgc tggctcgact
      241 acacgcgcgt ggtgaaggcg atggcgaagc cgcaggtcgt cgacgcgcga aacctgctcg
      301 accccgcggc gatgcgtcgc ctcgggttca cgtaccaggg cgtggggcgc tgatgccgcg
      361 cgcggtgctc accggcggcg cgggattcct cggctcccac ctcgccaccg agctccggcg
      421 cggggggtac gacgtcgtcg ccgtcgacga tctctccacc ggtcgtgtcg agaacctcga
      481 ccacctcgcc gacgaccccg ggttcacgct gctcgagcac gacgtcagca ggggcatccc
      541 gatcgacggg ccggtcgccg cggtgctgca ctttgcgagc ccggcgagcc cgccggcgta
      601 cctcgcccgg cccctcgaga cgctcgccgt cggatcggaa ggcacccgtc atgcgctcga
      661 gctcgcgcgc gcacacggcg cgcggttcct gctcgcgtcc acgagtgaga tctacggcga
      721 tccgctcgtc catccgcagc ccgagtcgta tctcggcaac gtgaactccg ttggcccgcg
      781 cgcggtgtac gacgaagcga agcggttcgc cgaggcgatc acgatggcgt accaccgcga
      841 gtacggactc gacacgaaga tcgtgcgcat cttcaacacg tacggaccgc ggatggcgcc
      901 cggcgacggt cgcgtcgtca cgaacttcct cgagcaggcg ctcgccggcc ggccgctcac
      961 cgtgtacggc gacggcagcc agacccgatc gttctgctgg gtcgacgacg aagtgcgcgg
     1021 cttcctcgcg ttgctcgcgt ccgactggac cggccccatg aacgtcggga acccgggcga
     1081 gttcaccgtg ctggagctcg cgaagatcgt catcgaggtg acggcgtccg actccgagat
     1141 cgtgttcgag cccctgcccg tcgacgatcc cacccgacgt cgccccgaca tcacgctcgc
     1201 gcgcgacgtg ctcggatggg agcccgaggt cgacctgcgc acgggcgtcg cacgcctcgc
     1261 tgactggtac cggacacaac ggatgtcgtg aactcgcgct gggcggccgt cgtcgtcaac
     1321 tacgaagcgg gcgacgcgct ggtcgcgtgt gtccgctcgc tgctcgccga cgattccgcc
     1381 ggcgtgccgc ccgagctcgt cgtcgtcgac aacgggtcgc gcgacggctc cgttgcgacg
     1441 ctgcgacgcg agctcccggg catcacggtc atcgagccgc acgcgaactt ggggtacgcg
     1501 ggcgcggcga accgcggcat cgcgcgcacc accgcgccga tcgtcgcggt gtgcaacgcc
     1561 gacctcgtga tcgcaccggg tgcgggtgcg gtgatgctcg ccgcgttcgc cgacgacacc
     1621 gtcggcgcgg cggggccctg cattcgcaac ctcgacggct cggtgtaccc gtcggtccga
     1681 cgcgacgacg gggtcgtgga tgccaccggc cacgcggtcc tcggatccgt gtggccgacc
     1741 aacccgttca cgcgccgcta ccgggagctc gacgccgacc cgaccgtcgc gcgtgacgtc
     1801 gagtgggcgt cgggcgcggc gctgtggctc cggcgcgacg ccatcgaggg cgtcggtggc
     1861 tgggacgagg gcttcttcat gtatctcgaa gacgtcgacg tggggtggcg gctccggaag
     1921 gccgggtggc gagtgcggta cgaaccggcg gccgagatcg tccacatcca gggcctgtcc
     1981 accgaccagc accggacccg gatgatcctc gagcaccacc gctccacgta ccgcttcgcg
     2041 tcgaagcgct ggcaaggctg gcaacgcctg ctgctgatcc cgacggcggc gttgctcgcc
     2101 gtgcgcgccg ccttcaccgt ggcgtgggac cgcctgagac cgcgtcgaag ccgttccgac
     2161 atcacccggt aacctcggcg gccgtgccca agtcgcgcaa caagtactcg cgcgcgagga
     2221 tgcgcgagcg cagccggaag tcgcgccacc aacgcaccag cgggtggttc tacgccacgg
     2281 tcgcggtcat cgtcgtgatc ggaagcgtca cgattggcgc gatcgtgatg ggccgcgacg
     2341 acaacaacac ggcaccggtc atcggcgacc actggcacgc cgcgttcggc gtgaacatct
     2401 gcggcgagtg gatcagcaac ccggccacgt tcgagactcc cgccaacaac tctggtgtgc
     2461 gcgcgggcat ccacacccac ggcgacggct tcatccacat ccatccgttt gcgcgcgcgg
     2521 agaccggcaa caacgcgacg ctcggcaagt tcctctcgta cggcggctgg tcggcctccg
     2581 aggactcgat ctcggtctgg accggtcccg acgtcgacaa gagcaagacc acgtggacga
     2641 acggcgacaa gtgcccgagc gggtcggagg agggcgcggg cgaaccgggg cgtgtcgtgt
     2701 tcgaggtgaa ctgcaagacg gtggagggca atccgtccga tcacaagctc gccgaccagg
     2761 aggtcgtggc gatcggcttc gtgccgaagg gcgtcgagat cggcgcgccg cccaacgcgg
     2821 cgagcgcccc ggagaacgac agcgggaccg gcaccacacc cgaggcgatc aaccagaagg
     2881 agtgccgtcc gtcggccgag aacaacccgg gcgtgcccga gacggtcccg cctgcgacca
     2941 ccgcgacgac gacgccgtcg accaccagcc ccaccccgtg aaagcggtcg tcctcgtagg
     3001 gggcgagggc acccggctcc ggccactcac gtacgcgacc ccgaagccgc tgctgccgat
     3061 cgcgaaccag ccgttcctgg agcgccagct cacatggatg caccggcacg gcatcgacga
     3121 ggtcgtgctc tcactcgggt atctccccga cgcgttcgca cagcacttcg cacatggcag
     3181 cttcggcgac gttcgcttgc agtacgcggt cgaagacgag ccgctcggta ctgcgggtgc
     3241 gatccgctac gcggccgagg tcggcgggat cgacgagcgg ttcgtcgtct
//
LOCUS       DASUGA010000253         2451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_695077, whole genome shotgun sequence.
ACCESSION   DASUGA010000253 DASUGA010000000
VERSION     DASUGA010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695077"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VFX21_04985"
     CDS             complement(<1..411)
                     /locus_tag="VFX21_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095342.1"
                     /translation="MAGIHISTRVDADAFAVFVRDDAGVEISNATVTVTRRRVDGTGE
                     PVTKQGDLYPLTTDLCAYDIKASDGTNEVSDNIFAKSSVTTPAFGEYDKNFRDVALQL
                     AFTSAGVLVIVFLLGAIVWFWGLDPATRAQPAPLS"
     gene            complement(493..1077)
                     /locus_tag="VFX21_04990"
     CDS             complement(493..1077)
                     /locus_tag="VFX21_04990"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5095343.1"
                     /translation="MRSRLTQRGRDRREQLIACAARLFAERGYHPTSVSDIVEELGVG
                     KGVFYWYFSSKEELLAELLKASNHDLRKRQQVAIGDEPEPVRRIELGIRASLEWFHEH
                     RDYFAIIQFAATDETFAPVLHRNREIAIADTIRHLKDGIVEGRIADQDPEMLAHAIHG
                     VVSELTRVYLIEGNESVDRVNDLAVSFCLSGLTR"
     gene            1325..1477
                     /locus_tag="VFX21_04995"
     CDS             1325..1477
                     /locus_tag="VFX21_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095344.1"
                     /translation="MLSRIRFELGRGRALANARHELDRVEQSLAAIDALGARLAARGA
                     AVQRAA"
     gene            1496..2206
                     /locus_tag="VFX21_05000"
     CDS             1496..2206
                     /locus_tag="VFX21_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013475739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5095345.1"
                     /translation="MLYWVIKGCLTPVLRLGFRIKVEGREHLPKNGPVILASNHRSFL
                     DSIFIPLVVRRRVTFVAKAEYFDDPKSAWFFKGVGQIPIRREGGSASERALDSAMDVL
                     REGGVFGIYPEGTRTRDGYLHRGHTGVARLALRAGAPIVPVGLIGTDDIQPVDKRMPR
                     FGKPVTIRFGEALDAARYAGREHDHLALRELTDELMYEIGQLSGYEYVDTYATRKAED
                     LPTEVAHVASYTDARAAS"
     gene            complement(2208..>2451)
                     /locus_tag="VFX21_05005"
     CDS             complement(2208..>2451)
                     /locus_tag="VFX21_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HEX5095346.1"
                     /translation="QFQADMIDAKVSRAATQETTALGAAFLAGLAEDVWGSPATVNAT
                     WRADATFTPAMDAAARTQRRSEWSRAVERSRDWARE"
BASE COUNT          379 a          841 c          824 g          407 t
ORIGIN      
        1 gctcaacggc gccggttgtg cgcgcgtcgc gggatcgagt ccccagaacc acacgatggc
       61 gccgagcagg aacacgatga cgaggacgcc cgccgatgtg aacgccagtt gcagtgcgac
      121 gtcgcggaag ttcttgtcgt actcgccgaa ggcgggcgtc gtgacgctcg acttcgcgaa
      181 gatgttgtcg gacacttcgt tggtcccgtc ggacgccttg atgtcgtacg cgcacaggtc
      241 ggttgtgagc ggatagaggt cgccctgctt cgtcacgggc tccccggtgc cgtcgacccg
      301 tcggcgcgtg acggtgacag tcgcgttgct gatctcgact ccggcgtcgt cgcgaacgaa
      361 cacggcgaac gcgtccgcgt cgacgcgagt ggagatgtga atgcccgcca tggaggcacc
      421 tcccgctcgg agagacgccg cgaaggctag gcggttccgg cgacggcgac catcccggac
      481 gcgggtacgc agctagcggg tcaggccgct caggcagaag ctgaccgcga ggtcgttcac
      541 gcggtcgacc gactcgttgc cctcgatcag gtagacgcgg gtcagctcgg agaccacgcc
      601 atggatcgcg tgcgcgagca tctcgggatc ctggtcggcg atgcggcctt cgacgatgcc
      661 gtccttcaag tgacggatcg tgtccgcgat cgcgatctcg cggttgcggt gcaacaccgg
      721 tgcgaacgtc tcgtcggtcg ccgcgaactg gatgatcgcg aagtagtcgc ggtgctcgtg
      781 gaaccactcg aggctggcgc ggatgccgag ctcgatgcgg cgcacgggtt cgggttcgtc
      841 gccgatcgca acttgctggc gcttgcgcag gtcgtggttc gacgccttca acaactcggc
      901 gaggagctcc tccttcgagg agaagtacca gtagaagacg cccttgccga cgccgagctc
      961 ttcgacgatg tcggacaccg aagtcgggtg atagccgcgc tcggcgaaca gccgggccgc
     1021 gcacgcgatc agctgctcgc gacggtcacg cccgcgctga gtgagacggc tgcgcatcgc
     1081 gagaacgcta ctcgccttct tgaccgcttg gtcaagagcc atgtttcttt gtcgctccgg
     1141 tcgtgctcgc gtgaggcggc ccggattgtt ggcccgccct tcgctccggc gagctcgctg
     1201 gcgctcgccg cgctttcgga gtcggaccgt cccgtcatgc aggggtcacg agctgttcga
     1261 acgcctgtga tccgccagga acgccctggt cacgtccgtc ccgcacactg cgatcatgcg
     1321 aaccatgctc agccggatcc gattcgaact cgggcggggc cgtgccctcg ccaacgcgcg
     1381 ccacgagctc gaccgtgtcg agcagagcct cgcggccatc gatgccctcg gggcccgcct
     1441 cgccgcccgc ggcgcagcag tacaacgcgc ggcatgactc gcgtaccctg cgcgggtgct
     1501 ctattgggtc atcaagggct gcctcacccc ggtcctgcgc ctcggtttcc gcatcaaggt
     1561 cgaagggcgc gagcacctgc ccaagaacgg tccggtcatc ttggcgagca atcaccgctc
     1621 gttcctcgac tcgatcttca tcccgctggt cgtgcgccgc cgggtgacgt tcgtcgccaa
     1681 agccgagtac ttcgacgacc cgaagtccgc gtggttcttc aaaggcgtcg gccagatccc
     1741 catccgcaga gaaggaggca gtgccagtga gcgggccctc gacagcgcga tggacgtctt
     1801 gcgggaaggt ggcgtgttcg gcatctatcc cgaaggcaca cgcacccgcg acggctacct
     1861 ccatcgcggc cacacgggag tcgcgcggct cgcgctgagg gcgggcgcgc cgatcgtgcc
     1921 cgtcgggctc atcgggaccg acgacatcca gccggtcgac aagcgcatgc cgcgcttcgg
     1981 caagccggtg acgatccgct tcggcgaagc gctcgatgcc gcgcgctacg cgggacgcga
     2041 gcacgaccat ctcgccctgc gcgagctcac cgacgagttg atgtatgaga tcggccagct
     2101 gtcgggctac gagtacgtcg acacgtacgc aacccgcaag gcggaggatc tccccaccga
     2161 ggtcgcgcac gtcgcgtcgt acaccgacgc ccgcgccgcg tcctgaccta ctcccgcgcc
     2221 cagtcgcgcg accgttcgac cgcgcgggac cactcgctcc gccgctgcgt gcgcgccgcc
     2281 gcgtccatcg ccggtgtgaa cgtcgcgtcg gcacgccacg tcgcgttgac ggtggcgggg
     2341 gagccccaga cgtcttcggc gagccccgcg aggaacgcgg cgccgagcgc cgtcgtctcc
     2401 tgtgtcgcag cccgcgacac cttggcgtcg atcatgtcgg cttggaactg g
//
LOCUS       DASUGA010000254         2260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_842087, whole genome shotgun sequence.
ACCESSION   DASUGA010000254 DASUGA010000000
VERSION     DASUGA010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842087"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2223)
                     /locus_tag="VFX21_05010"
     CDS             complement(<1..2223)
                     /locus_tag="VFX21_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEX5095347.1"
                     /translation="MALASSTTALTGACPAGVVDAVSFGGTASDCGAKTTQTLTNSTA
                     AIRGDAGCAYTKDLSVDFSTGAPAPRNSASPLHVCPGALPVGDLDHVTIAGSATAFVG
                     GTTQLTATPQDANGQTVTTATTTWSSSDDAIATVDATGLVTGVAESATPVTITATSID
                     NDLTVSASVAITVTTPTINWLDVGWNTTSLPPGFQANMFLTARTASQGTIIPATFTVE
                     ALDPAIADVLPVPNAIMIQAVAASPSKPRFRITASPIAGGTPYVFTTGASTSITVETP
                     TNAPIADYSPNDEFGDPTPANAATPNDFLITRPQYVISYNQSRGTPNWVAYELDNRQF
                     GAEDRCNCFTADPDLPSDRQIFTSDYTNGGYDRGHMARSADRTAANVDNATTFYLSNI
                     VPQKADLNQGVWAQFENALGDSAKAGRAVYIVTGPLYSSSHPLVFLKNEGKVAVPDST
                     WKVALIGPMNGGAPFTRGNVQGWNDVSGLSVLAVNMPNDSGVRNDPWQKYLTTVDRIE
                     QSTGYDVLSLLEASFQDALEAGDRSPTAAFSFSGTPKEGSAIAFDASASTDPDLGRTD
                     LGRTENLTYAWQFGDGTSATGETPSHSFTNDGDFTVTLTVSDAFGWQKTLSQTVSIAN
                     VAPFVTLNATSPVSILSGDAVSVAGSFTDPGNDAPWQSVIAWGNGTATSATHAQSGAS
                     ITGTKSYLAAGTYTVSLTVTDNDGASGVQSLSVDVARRPVSGTATPGSIQMTDLAGDV
                     K"
BASE COUNT          318 a          713 c          833 g          396 t
ORIGIN      
        1 cttgacgtcg ccggcgaggt cggtcatctg gatcgagccc ggtgtggcgg tgccggaaac
       61 gggccgacgc gcgacgtcca ccgagagcga ctgcacgccg ctcgcaccgt cgttgtcggt
      121 cacggtgagc gagacggtgt atgtgccggc ggcgagatag ctcttcgtcc ccgtgatcga
      181 ggcgcccgac tgcgcgtgtg tcgccgaggt ggccgtgccg ttcccccagg cgatgacgga
      241 ttgccacgga gcgtcgttcc ccggatcggt gaagcttccc gcgacgctca cggcgtctcc
      301 cgacaggatc gacaccggcg acgtcgcgtt caacgtcacg aagggcgcga cgttggcgat
      361 cgagacggtc tgcgagagtg tcttctgcca cccgaacgcg tcgctcacgg tgagtgtgac
      421 ggtgaagtcg ccgtcgttcg tgaacgagtg gctcggggtc tcgccggtgg ccgaagtgcc
      481 gtcaccgaac tgccacgcgt aggtgaggtt ctcggtgcga ccgaggtcgg tgcggccgag
      541 atcgggatcg gtgctcgccg acgcgtcgaa ggcgattgcg gagccttcct tcggcgtgcc
      601 gctgaacgag aacgccgcgg tgggcgaccg gtcgccggcc tcgagcgcgt cctggaagct
      661 ggcctccagc agcgagagga cgtcgtagcc ggtcgactgc tcgatcctgt cgaccgtggt
      721 gagatacttc tgccacggat cgttccgcac gccggagtcg ttcggcatgt tgacggcgag
      781 cacgctgagt cccgacacgt cgttccagcc ctggacgttg ccgcgcgtga acggtgcgcc
      841 gccgttcatc gggccgatga gcgcgacctt ccacgtgctg tccggcaccg cgaccttgcc
      901 ctcgttcttg aggaagacga gcgggtgcga cgacgagtag agcggaccgg tgacgatgta
      961 aacggcgcga ccggccttcg ccgaatcgcc gagcgcgttc tcgaactgcg cccagacacc
     1021 ctgattcagg tcggccttct gcgggacgat gttgctgaga tagaaggtcg tcgcgttgtc
     1081 gacgttcgcc gccgtgcgat cggccgagcg ggccatgtgg ccgcggtcgt atccgccgtt
     1141 ggtgtagtcc gacgtgaaga tctgcctgtc cgacggaaga tccgggtcgg cggtgaagca
     1201 gttgcagcgg tcctcggcgc cgaactgacg gttgtccagc tcgtacgcga cccagttggg
     1261 ggtgccgcgc gactggttat acgagatcac gtactgcggc cgggtgatca ggaagtcgtt
     1321 cggcgtcgcc gcgttcgcag gagtcggatc gccgaactcg tcgttcggcg agtagtccgc
     1381 gatcggcgcg ttggtcggcg tctcgacggt gatcgacgtc gacgcgcccg tggtgaacac
     1441 gtacggcgtg ccgccggcga tcggcgacgc ggtgatcctg aagcgcggct tcgacggcga
     1501 cgccgcgacc gcctggatca tgatggcatt cggcaccgga agcacgtcgg cgatcgccgg
     1561 gtccagcgct tcgacggtga acgtggcggg gatgatcgtg ccctggctgg cggtgcgcgc
     1621 cgtcaggaac atgttcgcct ggaagccagg cggcaggctc gtcgtgttcc atccgacgtc
     1681 gagccagttg atcgtcggtg tcgtcacggt gatggcgacg gacgcggaca cggtgaggtc
     1741 gttgtcgatc gacgtcgcag tgatggtgac gggggtagcg ctctccgcca cgccggtcac
     1801 gaggccggtc gcgtccaccg tggcgatcgc gtcgtcgctc gacgaccagg tcgtcgtcgc
     1861 cgtcgtcacc gtctgcccgt tcgcgtcctg tggcgtggcg gtgagctgcg tcgtaccgcc
     1921 gacgaaggcg gtcgccgaac cggcgatggt gacgtggtcg agatcaccga cggggagcgc
     1981 gccgggacag acgtggagcg gcgacgcgct gttgcgcggc gccggcgcgc cggtggagaa
     2041 gtcgaccgag aggtccttcg tgtacgcgca tcccgcgtcg ccgcggatcg cggcggtgct
     2101 gttggtgagc gtctgcgtcg tcttcgcgcc gcagtcgctc gcggtgccgc cgaagctcac
     2161 ggcatcgacg acgccggcgg gacatgcacc ggtgagtgcc gtggtggacg acgccagcgc
     2221 cacctttccc gccgtcgcgc ccatcgcgat cgtgccggtc
//
LOCUS       DASUGA010000255         3963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_429310, whole genome shotgun sequence.
ACCESSION   DASUGA010000255 DASUGA010000000
VERSION     DASUGA010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429310"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..858)
                     /locus_tag="VFX21_05015"
     CDS             complement(40..858)
                     /locus_tag="VFX21_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX5095348.1"
                     /translation="MIPAAFEYVRAGSADEAVALIVEHGDEAKFLAGGMSLLPLMKLR
                     LATPAVLVDLGRVKDLSYIRDAGDHLAIGALTRHRDIEISDVLAKECGVLRAVAAQVG
                     DNQVRHRGTIGGSVVHGDPASDLPAALLALDATFVGKGPNGERKISATEFFQGFLETA
                     LGEDELLTEIQVRKSGSDGFNFQKFNRRAQDWAIVGAVAARVNGGTHVALVNMGATPL
                     RAHAVETALSQGADAATAARDAAMGTEPPSDLNASPEYREHLARVLVQRALEAL"
     gene            complement(855..1325)
                     /locus_tag="VFX21_05020"
     CDS             complement(855..1325)
                     /locus_tag="VFX21_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5095349.1"
                     /translation="MRIGMQVNGAAQEHDVEPRTLLVQYLREACALTGTKIGCDTSSC
                     GACTVLVDGESVKSCTMLAAQAEGSSITTIEGLAKGDEWHPVQTAFHENHGLQCGYCT
                     AGMVMASVSLLEEIPNPTEKDVRLGLEGNLCRCTGYHNIVKSVLAAAEKMQAAR"
     gene            complement(1350..1760)
                     /locus_tag="VFX21_05025"
     CDS             complement(1350..1760)
                     /locus_tag="VFX21_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009741982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX5095350.1"
                     /translation="MDIAAALRFAAENHHAVLATRRRDGRPQLSPVAVGVDDDGHIVI
                     STREPSMKVKNLRRDPHASLCLLNDAFFGAWAQVEGPAEIVSLPEAMDGLIAYYRSVS
                     GEHPDWDDYRDAMQRDRRCLIRITPERAGPTVSG"
     gene            complement(1774..2229)
                     /locus_tag="VFX21_05030"
     CDS             complement(1774..2229)
                     /locus_tag="VFX21_05030"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5095351.1"
                     /translation="MIEVGQAAPDFTLPGIQNGEKRDYTLSEYRGKNVILAFYPGDFT
                     PGCTRQMCSYRDNLEEIDKTGAVLLGISPQDVDSHEKWAEKKSFAFPLLADCDKTVIE
                     AYGVGAPIINVRRSVFVVDGEGVVRFTDRKLIGATFVPVDKLVEVVTSL"
     gene            complement(2229..2978)
                     /locus_tag="VFX21_05035"
     CDS             complement(2229..2978)
                     /locus_tag="VFX21_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX5095352.1"
                     /translation="MSGHSKWHSIKHKKGAADAKRGKLFAVLIRQIEVAARAGGGDLD
                     SNATLRTMVQKARDNSVPMDTIQRAIKRGTGEEEGVTYESVAYEGYAPGGIAVIVQAL
                     TDNRNRTSAEIKNLFSRNGGSFAEPGAVSWQFERKGIVNVDKAVDEDELMLVAADAGA
                     DDVEDGGDAWNVTTPPTELHAVRTAIEDAGYKVASSDLTMVPTTTVEVGEESQAKTIL
                     RLMDALEDHDDVEAVYANFDIPDAMLEAVTA"
     gene            complement(2978..3553)
                     /gene="pdxT"
                     /locus_tag="VFX21_05040"
     CDS             complement(2978..3553)
                     /gene="pdxT"
                     /locus_tag="VFX21_05040"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEX5095353.1"
                     /translation="MPKIGVLALQGAFREHAEALTALGADVALVKRPEHLAGIDAIFL
                     PGGESTTIDKLLDSSGLRTPLTQQLRDGLPAFATCAGLIVCATDIVDGRPDQLPIGVL
                     DVTVRRNGYGRQNESFEAPLDVRDLPGGAFPGVFIRAPLVERTGSTVEVLAEHEGVPV
                     LGRQGVVWFASFHPELANDLRLHQMFLSEVG"
     gene            complement(3582..>3963)
                     /locus_tag="VFX21_05045"
     CDS             complement(3582..>3963)
                     /locus_tag="VFX21_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX5095354.1"
                     /translation="ILGDIEKLKVARAEERYGWAKELRAPVDLVDEVAERGELPVPLF
                     CAGGIATPADAALVMQLGAQANFVGSGIFKSEDPAKRARAIVEATTHYADPDIIAKVS
                     RGLGEAMRGDEIDKLDTKLADRGW"
BASE COUNT          616 a         1350 c         1307 g          690 t
ORIGIN      
        1 agcgggtccc gagacccgcg gccaagacaa ccgggatgat caaagagctt cgagcgcgcg
       61 ctgcacgagc acgcgcgcaa gatgctcgcg gtactcgggc gacgcgttca ggtcgctcgg
      121 cggctccgtc cccattgccg cgtcgcgcgc agcggttgcg gcgtcggcac cctgcgacaa
      181 cgcggtctcg accgcgtgcg cccgcaacgg cgtggcgccc atgttcacga gtgcgacgtg
      241 ggtgccgccg ttcacacgag cggcgaccgc tccgacgatc gcccagtcct gggcccgccg
      301 gttgaacttc tggaagttga agccgtcgct accgctcttg cgtacctgaa tctcggtcag
      361 cagctcgtct tcaccgagcg cggtttcgag gaagccttgg aagaactcgg tcgccgagat
      421 cttgcgctcg ccgttcggcc ccttcccgac gaaggtggcg tcgagcgcga gcaacgcagc
      481 gggcaggtcg ctggcgggat cgccgtggac caccgagccc ccgatggtgc cgcggtgtcg
      541 aacctggttg tcgccaactt gcgcggcgac cgcgcgcagc acaccgcact ccttggcgag
      601 cacatcgctg atttcgatgt cgcggtgacg cgtgagcgcg ccgatcgcga gatggtcgcc
      661 ggcatcgcgg atgtaggaaa ggtccttcac gcgcccgagg tcgaccagca ccgcgggcgt
      721 ggcgaggcgg agcttcatca acggcagcaa cgacatgccg ccggcgagga acttcgcttc
      781 gtcgccgtgc tcgacgatca gcgcgaccgc ttcgtcggcc gagccggccc ggacgtattc
      841 gaacgcggcc gggatcatcg ggcggcctgc atcttctcgg cggcggccag gaccgacttc
      901 acaatgttgt ggtaaccggt gcaacggcag aggttgccct cgaggccgag ccgcacgtcc
      961 ttctcggtgg ggttcgggat ctcttcgagc agcgacaccg acgccatcac catgccggcg
     1021 gtgcagtagc cgcactgcag gccgtggttc tcgtggaacg cggtctgcac cggatgccat
     1081 tcgtcaccct tggccaggcc ctcgatcgtg gtgatgctcg acccctcggc ctgcgccgca
     1141 agcatcgtgc acgacttcac cgactcaccg tcgaccagca ccgtgcacgc gccacaactg
     1201 gacgtgtcgc acccgatctt cgtccccgtc agcgcgcacg cctcgcgcaa gtactgcacg
     1261 agcaaggtgc gcggttcgac gtcgtgctcc tgcgccgcgc cgttgacctg cattccgatc
     1321 cgcatgtcgg cggagactag cggccgcgac taccccgaca ccgtggggcc ggcgcgctcg
     1381 ggcgtgatgc gaatcaggca gcgacggtcg cgttgcatcg cgtcgcgata gtcgtcccag
     1441 tcgggatgct cgcccgagac actgcggtag tacgcaatca ggccatccat ggcttcgggc
     1501 agcgacacga tttcggcggg cccctcgacc tgcgcccatg cgccgaaaaa tgcgtcgttc
     1561 agcaggcaga gcgacgcatg cggatcgcgg cgcaggttct tcaccttcat cgaaggctcg
     1621 cgggtgctga tgacgatgtg gccgtcgtcg tcgacgccga ccgccaccgg cgagagctgg
     1681 ggccggccgt cgcggcggcg ggtcgccagc acggcgtgat ggttctcggc ggcaaagcgc
     1741 agggcggccg cgatgtccat attgccccct ttgtcagagg ctggtaacga cctcgacgag
     1801 cttgtcgacg gggacgaagg tcgcgccgat gagcttgcgg tcggtgaagc gcacgacccc
     1861 ttcgccgtcg acgacgaaga ccgatcgacg cacgttgatg atcggcgcgc ccacgccgta
     1921 ggcctcgatg accgtcttgt cgcagtcggc gagcagcggg aacgcgaagc tcttcttctc
     1981 ggcccacttc tcatggctgt cgacgtcctg gggcgagatc ccgagcagca ccgcgccggt
     2041 cttgtcgatc tcttcgaggt tgtcgcggta cgagcacatc tggcgcgtgc aaccgggcgt
     2101 gaagtcgccc ggatagaacg cgaggatcac gttcttcccg cggtactcgg agagcgtgta
     2161 gtcacgcttc tcgccgttct gaataccggg aagcgtgaag tccggtgcag cctgccctac
     2221 ttcaatcatt acgcggtcac cgcctcgagc atcgcgtcgg ggatgtcgaa gttggcgtac
     2281 accgcttcga cgtcgtcatg gtcttccagc gcgtccatca agcgcaagat ggtcttggcc
     2341 tggctctcct cccccacttc caccgtcgtg gtggggacca tcgtgaggtc ggaggacgcc
     2401 accttgtagc ccgcgtcttc gattgcggtg cggaccgcgt gcagttcggt gggcggggtc
     2461 gtcacgttcc acgcgtcgcc gccgtcttcg acatcgtcgg cgccggcgtc agcagccacg
     2521 agcatgagct cgtcttcgtc gaccgccttg tcgacgttca caatgccttt gcgttcgaac
     2581 tgccacgaca ccgcgcccgg ttcggcgaac gagccgccgt tgcggctgaa caggttcttg
     2641 atctcggccg acgttcggtt gcggttgtcg gtcagcgctt gcacgatcac tgcgatgccg
     2701 cccggcgcgt agccctcgta ggccaccgac tcgtaggtga cgccttcttc ctcgccggtc
     2761 ccccgcttga tcgcgcgctg gatcgtgtcc atcggcaccg agttgtcgcg cgccttctgc
     2821 accatcgtgc gcaacgtcgc gttcgagtcg agatcaccgc cgccggcacg cgctgccact
     2881 tcgatctggc ggatcaagac cgcgaagagc ttgccgcgct tggcgtcggc ggcacccttc
     2941 ttgtgcttga tcgaatgcca cttggaatgg ccggacatca acccacctca cttaggaaca
     3001 tctggtgtag acgaaggtcg ttcgcgagct ccgggtggaa cgacgcgaac catacgactc
     3061 cttggcggcc gagcaccgga acgccctcgt gctcggccag cacctcgacc gtcgagccgg
     3121 tgcgctcgac caggggcgcc cggatgaaga caccgggaaa cgcaccgccc ggcagatcgc
     3181 gaacgtcgag cggcgcctcg aacgactcgt tctggcggcc gtagccgtta cggcgcacgg
     3241 tcacatcgag caccccgatc gggagttgat cgggccggcc gtcaacgatg tcggtcgcgc
     3301 acacgatgag ccctgcgcac gtcgcgaatg ccggcaagcc gtcgcggagt tgctgggtca
     3361 ggggcgtgcg cagccccgac gaatcgagca gcttgtcgat ggtcgtcgac tcgccgccgg
     3421 gcaggaagat cgcatcgata cccgcgaggt gttcggggcg tttgacgagc gcgacgtcag
     3481 cgccgagcgc ggtgagcgcc tccgcgtgtt cgcggaacgc gccctgcaat gcgagcactc
     3541 caattttcgg catggcctgg cggcctccgc gcacccacgc gctaccagcc gcggtcggcg
     3601 agcttggtgt cgagcttgtc gatctcgtcg ccgcgcatgg cctcgccgag accgcgcgac
     3661 accttggcga tgatgtcggg gtcggcgtag tgcgtcgtcg cctcgacaat tgcccgggcg
     3721 cgcttggcgg ggtcttccga cttgaagatg ccactgccaa cgaagttcgc ctgcgcgccc
     3781 agttgcatca cgagcgcggc gtcggcgggc gtagcgatac cgcccgcgca gaacaacgga
     3841 acgggcagct cgccgcgctc ggcaacttcg tcgacgaggt cgaccggcgc acgcagctct
     3901 ttggcccagc cgtagcgctc ctcggcgcgc gcaaccttca gcttctcgat gtcgccgagg
     3961 atc
//
LOCUS       DASUGA010000256         2259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1418172, whole genome shotgun sequence.
ACCESSION   DASUGA010000256 DASUGA010000000
VERSION     DASUGA010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418172"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFX21_05050"
     CDS             <1..297
                     /locus_tag="VFX21_05050"
                     /inference="COORDINATES: protein motif:HMM:NF024687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX5095355.1"
                     /translation="GNIDTRVLSSVLLRALARSGRVIRLQIEVPDRPGVLAMVADIVG
                     SCRGNIVDVAHHRDQPGVAVKRAMLELSIETRDRSHADEIVNALTSAALRVTRA"
     gene            336..1274
                     /locus_tag="VFX21_05055"
     CDS             336..1274
                     /locus_tag="VFX21_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5095356.1"
                     /translation="MRKPFVVVVTTLMLAGLAACGSSSKDSEGSTNTTTAASGNTSTS
                     TPAKATPIDCNGTNAYIEDIGDAADFKPVKADTLSVVTSLPGPGFWEGSDEDPSKVTS
                     GYEYDIAKAMQKAFGLKNLEVRNVSFDAIVAGTATDYDVSLSQVTITCERAKVVKFSQ
                     PYFFSNQGVLVKSDFDKPIATVEDAKKLKWGVQTATTALTLLEKIGPDSEPRSYTQLT
                     DGYTALEAGQVDAFLIDTAINLGQAARSNGALKVPAQFLQEGGPDQYGAILPKDSTNG
                     PAINAVFDELQKSGELSKLVTKNLTTDPGVLPIIVP"
     gene            1387..>2259
                     /locus_tag="VFX21_05060"
     CDS             1387..>2259
                     /locus_tag="VFX21_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX5095357.1"
                     /translation="MAITWAMLWKFDQSLVVNSAQQTTARILEVIIALATLAVPWFGI
                     RALAASLTASKLAREDERHSANIELADSRDRILIVVGLGLTALVVAFAAFLLAANDGS
                     VRRVLFNWDLIWRSRTALLRGFWLTIRLVIVAEAIVLVWALVVAVIRLLPGRACAPIR
                     FLAIVYTDVFRGIPAIVTIYLIVFGFSLAGVPPFNSLEGDTKLFWLAVFALVLVYGAY
                     VGEVYRSGLESVHWSQSAAARSLGLSQWQTLRHVVIPQAVRRILPPLMNDFVALQKDT
                     SLAFVVGALELTGQA"
BASE COUNT          395 a          745 c          702 g          417 t
ORIGIN      
        1 ggcaacatcg acacccgggt gctgagttcg gtgctgctgc gcgccctcgc ccggtcgggg
       61 cgggtcatcc ggctccagat cgaggttccg gaccggcccg gcgtcctggc aatggtcgcc
      121 gacatcgtgg gctcgtgccg gggcaacatc gtcgacgtgg cccaccaccg cgaccagccc
      181 ggcgttgccg taaagcgggc aatgctcgaa ctttcgatcg aaacgcgcga tcgatcgcat
      241 gctgacgaga tcgtgaatgc cctcacatcg gcggcgctgc gcgtgacccg ggcgtgagtt
      301 ctagagtgcc gccgttcccg gcgaaaggat cacacgtgcg taagccgttt gtcgtcgttg
      361 tgacgacgct catgctggcg ggcctcgcgg cctgcggcag tagtagcaag gactccgaag
      421 gttcgacgaa tacaacgact gcggcgtcgg gcaacaccag cacgtcgacc ccggcgaaag
      481 cgacgccgat cgactgcaac ggcacgaacg cgtacatcga agacatcggc gacgcggctg
      541 acttcaagcc ggtcaaggcc gacaccctga gcgtcgtcac cagcttgccc ggtcccggct
      601 tctgggaggg cagcgacgaa gacccgtcga aggtgacgtc cggttacgag tacgacatcg
      661 ccaaagccat gcagaaggcg ttcgggttga agaacctcga agtgcgaaac gtcagcttcg
      721 atgcgatcgt ggccggcacc gcgaccgact acgacgtctc gctctcgcag gtgaccatca
      781 cgtgcgaacg cgccaaggtc gtgaagttct cgcagccgta cttcttctcg aaccagggtg
      841 tactcgtgaa gtccgacttc gacaaaccga tcgcgacagt cgaggatgcc aagaagctga
      901 agtggggtgt gcagaccgcg acaaccgcgc tgacgctcct cgagaagatc gggcccgatt
      961 cggaaccgcg ttcgtacacg caactcacgg acgggtacac ggcgctcgaa gccgggcagg
     1021 tcgacgcgtt cctcatcgac actgcgatca acctcggcca agcggcgcga tccaacggtg
     1081 cactcaaggt gccggcgcag ttcctccagg aaggtggtcc cgaccagtac ggcgcaatcc
     1141 ttccgaagga ctccacgaac ggtccggcca tcaacgcagt gttcgacgag ttgcagaagt
     1201 cgggagagct ctcgaagctg gttacgaaga acctcacgac cgaccccgga gttctgccga
     1261 tcatcgttcc ctgaggtggt ttccgacgac ctcatcgcag tcgcaccgcc cggccaacgc
     1321 cgggcggtgc cgttccgagg aaacctcgcg accgcgacct tcaccgcagt tgcgtcggtc
     1381 gccgtggtgg cgatcacgtg ggcgatgctg tggaaattcg accagtcgct cgttgtgaat
     1441 tcggcgcagc agacgactgc ccgaatcctt gaagtgatca ttgccctcgc gacgctggct
     1501 gtgccttggt tcggaattcg cgcgctcgcc gcgtccctga cggcgtcgaa gctcgcgcgc
     1561 gaggacgaac gccatagcgc gaacatcgag ctcgctgatt cgcgcgaccg catcttgatc
     1621 gtcgtcggac tcgggctcac cgcactcgtc gtcgccttcg cggcgttcct cttggcggcc
     1681 aacgacggca gcgttcgccg cgttctcttc aactgggatc tgatctggcg gagcaggacc
     1741 gcattgctac gcggcttctg gctcacgatc cggctcgtca tcgtcgccga ggcgatcgtg
     1801 ctcgtgtggg cacttgtcgt cgccgtgatt cggctcctgc ccggccgcgc ctgcgcgccg
     1861 attcggttcc tggcgatcgt ctataccgac gtgtttcgcg gcatccccgc catcgtcacg
     1921 atctacctca ttgtcttcgg gttctcgctc gccggagtgc cgccgttcaa ttcgctcgag
     1981 ggcgacacca agctcttttg gctggccgtg ttcgccctcg tcctcgtgta tggcgcgtac
     2041 gtcggagagg tctaccggtc cggcctcgaa agtgtgcact ggagccagtc cgccgccgcc
     2101 cggtcgctcg ggctgtccca atggcagacg ctgcgccacg tcgtgattcc acaagccgtg
     2161 cgacgcatcc tcccgccact catgaatgat ttcgtcgcct tgcaaaagga cacgtcgctc
     2221 gcgtttgtcg tcggagcact cgaattgacc ggtcaggcc
//
LOCUS       DASUGA010000257         3080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1392855, whole genome shotgun sequence.
ACCESSION   DASUGA010000257 DASUGA010000000
VERSION     DASUGA010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392855"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="VFX21_05065"
     CDS             complement(<1..1356)
                     /locus_tag="VFX21_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095358.1"
                     /translation="MRTRVIAVTAALAALAIAWRLGTSEDAPPAPVANAARERAVDGV
                     RPSSGDSAVDGPLREPGGADNGAPGMGDAPPSRFDLRGTFVVVDHEGVEHAAESGEVE
                     VVIDGCSSGFDVRSGRWRLPDFVQRDRMRVTKATLGGKPVVCEIDIAIEPSNPALHLR
                     GRWLAPCRLRVVDGDTGLDLANVRVVENDTWAHWPAFATSDWRHPGGYQTDDASVVAA
                     RPVLLWPRPGEVRQYFIGAPDYAWTRIDIDPATTAERWVPLARGGDVDVTVTGRLPYD
                     ASLRASRDEGGRPLAEWTPHPDGATRLANLPAGTWHISVALGYWSEHPVVFADTRVEI
                     VPGTVATASLAARDVVEPHRVALEGTLVYPSAWRSPPRVDIDPMGEASAWNEGAWLTP
                     ERIEAAGDEYCWLAGMRTPGTYRARVIGSGFVTRFELSSTGGQNVRIVVPEPCDFRMR
                     VL"
     gene            complement(1524..2837)
                     /locus_tag="VFX21_05070"
     CDS             complement(1524..2837)
                     /locus_tag="VFX21_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095359.1"
                     /translation="MRLLAAVLLPTSVLAQDAPPARADLERAVAAYFAADDNATNSTL
                     AAAMKLARGNEALLIDVVTKKTFVARTTAAIDQRGIIDASYRFVADAPVPDGQRNNEA
                     LLAGPAGGDGLFPLVVYVPDATNTAGYGRELRADGIDAGRFVFLVPDEKRDNRYAPSL
                     HEHRRHAGPLRELLLTLPIDPDRVYMVGSGRGGHATWDVGLTCADRWAGIFPCNGGLV
                     HEGGWKASGGVFVGNARCFATFLVFNTSFDHGIVSCRYAVKKLREWNCRIDAVEEPQM
                     RVMSVGEAMGKLAPVVRNAHPRRIEKRFNHLVDGEHYWLRALDRKGKEWDPAAKILVK
                     DWPKDPAQQLETVWNAVEDACGHLAGTIAGNRFDVTAHGMGKVCVYLDPELVDFDAPV
                     TFAVNGKVVKTLKPARQLEVMLQHVRETGDTSRLYFAFVDLPVGP"
BASE COUNT          443 a         1104 c         1071 g          462 t
ORIGIN      
        1 gagcacgcgc atccggaagt cacacggctc cggcacgacg atgcgcacgt tctgtccgcc
       61 cgtcgacgac agctcgaagc gggtcacgaa gccactgccg atgactcgcg cgcggtacgt
      121 tccgggcgtt ctcatcccgg cgagccagca gtactcgtca ccggcggcct cgatgcgctc
      181 gggggtcagc catgcgccct cgttccacgc gctggcctcg cccatggggt cgatgtcgac
      241 gcggggcggg cttcgccacg cgctcggata cacgagcgtg ccctcgagcg cgacacggtg
      301 cggctcgacg acgtcgcgag ctgcgagcga cgcggtcgcc acggttcccg gcacgatctc
      361 gacgcgcgtg tctgcgaaga ccacggggtg ttcggaccag tacccgagag cgaccgagat
      421 gtgccacgta ccggcgggca ggttcgcgag ccgcgttgcg ccgtccgggt gcggcgtcca
      481 ctcggccagg ggccggccgc cttcgtcgcg gctcgcccgc agcgacgcgt catagggaag
      541 ccgcccggtg acggtcacgt cgacgtcgcc gccgcgcgcg agcggcaccc atcgctccgc
      601 agtcgtcgcg gggtcgatat caatgcgtgt ccacgcgtag tcgggcgcgc cgatgaagta
      661 ctggcgcacc tcacccggtc gcggccacag cagcacgggt ctcgccgcga cgaccgaggc
      721 gtcgtcggtc tggtacccgc ccggatgccg ccaatccgag gtggcgaacg cgggccagtg
      781 cgcccaggtg tcgttctcga ccacgcgcac gttcgcgagg tccaagccgg tatcgccgtc
      841 gacgacgcgc aggcgacagg gggcgagcca gcgtcctcga aggtgcagcg ctggattcga
      901 cggctcgatt gcgatgtcga tctcgcacac gacgggtttg ccgccgagcg tcgctttggt
      961 gacgcgcatc cgatcgcgct gcacgaagtc gggaaggcgc caccgccccg acctcacgtc
     1021 gaagccggag ctgcagccgt cgatcactac ctcgacctcc ccgctctccg cggcgtgctc
     1081 cacgccctcg tggtcgacga cgacgaacgt gccacgcagg tcgaagcgtg atggcggagc
     1141 gtctcccatc cccggcgcgc cgttgtcggc accgcccggt tctcgcaacg gtccgtcgac
     1201 cgcgctgtcg ccggacgagg gacgcacgcc gtcgaccgcg cgttcgcgag cagcgttcgc
     1261 gaccggcgca ggtggggcat cctcactcgt ccctagccgc cacgcgatcg ccagggcggc
     1321 gagcgcggcc gtgacagcga tgacgcgagt cctcacggcg acaatccaac ggaccgctgc
     1381 gcttggcgtt cagccgccgc cgggcaggtc gacgaacgcg aagtagagcc gcgacgtgtc
     1441 gccggtctcg cggacgcgaa gtagcgcgtc aggtcgtcga cgatcgccgc acggtccatg
     1501 gccgcactct accgctcggg tcgtcacgga ccgacgggca ggtcgacgaa cgcgaagtac
     1561 agccgcgacg tgtcgccggt ctcgcggacg tgctgcagca tgacttcgag ctggcgcgcg
     1621 ggcttcagcg tcttcacgac cttgccgttc acggcgaacg tgaccggcgc gtcgaagtcg
     1681 acgagctccg gatcgaggta gacgcacacc ttccccatgc cgtgcgcggt gacgtcgaag
     1741 cggttgccgg cgatcgtgcc ggcgagatgg ccgcacgcgt cctcgaccgc gttccacacc
     1801 gtctcgagct gctgcgccgg gtccttcggc cagtccttga cgaggatctt cgccgccggg
     1861 tcccactcct tgcccttgcg atcgagcgcg cgcagccagt agtgctcgcc gtcgacgagg
     1921 tggttgaagc gcttctcgat gcggcgcggg tgcgcgttgc ggaccacggg cgcgagcttg
     1981 cccatcgcct cgccgacgct catcacgcgc atctgcggct cctcgacggc gtcgatgcgg
     2041 cagttccact cgcgcagctt cttcaccgcg tagcggcacg acacgatgcc gtgatcgaag
     2101 ctcgtgttga acacgaggaa cgtcgcgaaa caccgcgcat tgccgacgaa cacgccgccg
     2161 ctcgccttcc acccgccttc gtgcacgagc ccgccgttgc acgggaagat gccggcccag
     2221 cggtccgcgc acgtgaggcc gacgtcccac gtcgcgtggc cgccgcggcc gctgccgacc
     2281 atgtagacgc ggtcgggatc gatcggcagc gtcagcagca gctcgcgcag cggaccggcg
     2341 tggcggcggt gctcgtgcag cgacggcgcg tagcggttgt cgcgcttctc gtcgggcacg
     2401 aggaacacga agcgtccggc gtcgatgccg tccgcgcgca gctcgcgacc gtagccggcc
     2461 gtgttcgtcg cgtccggcac gtagacgacg agcgggaaca gcccatcgcc gcccgccggc
     2521 cccgcgagca gcgcttcgtt gttgcgctgg ccgtccggca ccggcgcatc cgcgacgaag
     2581 cggtacgacg cgtcgatgat gccgcgctgg tcgatcgctg ccgtcgtccg cgcgacgaac
     2641 gtcttcttcg tcacgacgtc gatcagcagc gcctcgttgc cgcgcgcgag cttcatcgcg
     2701 gccgcgagcg tgctgttcgt cgcgttgtcg tccgcggcga agtaggccgc gacggcgcgt
     2761 tcgagatcgg cccgagccgg cggcgcatcc tgcgcgagga cgcttgtcgg caacaggacc
     2821 gcagcgagca ggcgcatcgg cgcatggtag tcggcatcgt ggtgcgcttc agatccgtga
     2881 aacggcaagc gcggctcggg cacagaatgc gcgcatgctc cgttccggcc tcgaccgcgc
     2941 gttcgcgcgc tacgcccgca ccggcgatcc cggggcgttg gcgaaggtgt tcgacggcag
     3001 tgctcaggag ctgtatcggc tcgggtatca cctgctcggc gaccggcacg cggcggagga
     3061 cctcgtgcag cacgcgttcg
//
LOCUS       DASUGA010000258         5208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2022794, whole genome shotgun sequence.
ACCESSION   DASUGA010000258 DASUGA010000000
VERSION     DASUGA010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2022794"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="VFX21_05075"
     CDS             <1..830
                     /locus_tag="VFX21_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX5095360.1"
                     /translation="GYWVARMAAALGATPTICAPIGGETGAVVKDLLAREGVALRSVT
                     GKADNSLFVHDRRHGDREALLELQPQPLGRHQLDQLYSVTLAAAMESGVCVVAGSQNA
                     TVVAADTYGRLVADLVECDVRVVVDLAGEPLRPALSGYPDVLKISHEELLADKWARDD
                     SPAELARAIAALQAAGARDVIVSRGANPSLANVDGELVEIIAPRMHEVDHRGAGDSMT
                     AGLAVNLARGKSLRDEGLALAAAAGAMNVTRHGLASGHAEMIEQLAQRVAIRPVRWR"
     gene            complement(817..1650)
                     /locus_tag="VFX21_05080"
     CDS             complement(817..1650)
                     /locus_tag="VFX21_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX5095361.1"
                     /translation="MAVLLGLCVAITYGASDFLGGFASKRSTSDSVVAMTQSIGFVIS
                     LVIVFLVGWDEVTGRDIGLSIGSGAGQVLGVVLLYRGLATGRMGVVAPVSAVTSALLP
                     ISYGLGTGEDPSALALFGVLLAIIAVAIVAAEPDHKSTGNDRSALALAIGAGLGFGTS
                     FIFFGETGEASGFWPVVIARLTAATLVVTFILAARRPRLPDRSERPVAVAAGVLDTTA
                     NGLLLFAVRGEMLSLVAPVASLYPASTVVLARFVLHEPIGRQRLAGLALAAVALVFIA
                     I"
     gene            1729..2592
                     /locus_tag="VFX21_05085"
     CDS             1729..2592
                     /locus_tag="VFX21_05085"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEX5095362.1"
                     /translation="MRIFVSVDMEGISGVTRWADVVKSGQDYERARRWLTADVNAAVA
                     GARAAGATDFVVEENHGTEMLCNVLLDEIDPEVEVVRGQPRGGATTMSALDDSFDAVF
                     LVGHHASSSDYPGVCAHTISHSHYKEVRLDGRPLTEGEMFALAAAQRGVPTALITGDD
                     VVTAILQKVCPGIEMAVVKRALSREAAAIIPPVRAQRIIHDAARRAVERLRAGEIDAP
                     EPHPPFAIEVELRESISKDIQATIAQRFPEFTVNDDRVFSFAVDEMAVGFRMAAIVQA
                     LAETPERVRSY"
     gene            complement(2586..3185)
                     /locus_tag="VFX21_05090"
     CDS             complement(2586..3185)
                     /locus_tag="VFX21_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5095363.1"
                     /translation="MVQLLRVPAPPERIELTGAYLRMVGVDDAPAVAAAVRANLEHLK
                     PWMAWADETACDAAFQRERLRGAVERWHTREEHQYGLFVPGDDTTFRGSFGLMTRRGR
                     GTLEIGYWLAADVTGQGHATRAAAALTDVALRVRGVRRSLVYTDEANVRSAAIPQRLG
                     YELLKIEVNAPTAPGESGRTQVWVRTKPIDQDLGRSVAQ"
     gene            complement(3233..4981)
                     /locus_tag="VFX21_05095"
     CDS             complement(3233..4981)
                     /locus_tag="VFX21_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5095364.1"
                     /translation="MKRPSLRRILREFSPFRRDGIGALVGIVVGVTCQVLSPLVVAYA
                     INQGVVDRNTGIIVGCVAVMATLIVGQVLGSYSELRAMGRFAERYLADLRSRLLGHLH
                     ELDLDYFSTEPAGKVVSRLTSDVESLQQFLQYGLSLLLRGILLLTLSALIMFTQSWQL
                     ALAVLVIVPVLYLAGRWYRPRAFGVQVGYRESIADLLNHVNEALIGMRVVQAYSIEKQ
                     QQREFASANDAALDAKIEAGRVTSIYYAVIEFMQPVALAIVIGYGAVLADRGEIEVGT
                     LIAFTLYLTRLFEPIQQFIELNSLMQAASAAYSRTFEFLDRQPMLRDQDGAREFRRGA
                     GEVHVDDVTFRYAPDAPPAVADLDIHLEPGERIAVVGTSGAGKSTLAKLIGRFYDPTA
                     GRVVIDGQDLRDVKHAPLRRDVMVVPQEGFLFDGTIADNIALGHPTATRAELLAACER
                     LGLADVLGRIPGGLDARIANRGLSLSSGQRQLVALARAFVADPAVIVLDEATSNLDPA
                     TDALVEHALVGLLDQRTSIVVAHRVGTALRADRVIVLEHGRIVEDGAPDELASREGAF
                     ARWVAAARAATTAAAS"
     gene            complement(4978..>5208)
                     /locus_tag="VFX21_05100"
     CDS             complement(4978..>5208)
                     /locus_tag="VFX21_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014048980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5095365.1"
                     /translation="SAVDPPTEAEIRRGLLHAMEGRTTLVIAHRVETISLAERVVLLH
                     EGRVVADGTHDELLALPEYRRALALETKELTS"
BASE COUNT          839 a         1762 c         1862 g          745 t
ORIGIN      
        1 agggctattg ggtcgcgcgc atggcggccg cgctcggcgc gacgccgacg atctgtgcgc
       61 cgatcggtgg tgagaccggc gccgtcgtca aggatctgct cgcccgcgaa ggggtggcgc
      121 tgcgatcggt gacggggaaa gccgacaact cgctgttcgt gcacgaccgc cgacacggcg
      181 atcgtgaagc gctgctcgag ctgcaaccgc agccgctcgg tcgccaccaa ctcgatcagc
      241 tgtacagcgt cacgctcgcg gccgcgatgg agtcgggtgt gtgcgtcgtt gccggatctc
      301 agaacgcaac ggtcgtcgcg gccgacacct acggtcgtct cgtcgcggat ctcgtcgagt
      361 gcgacgtgcg ggtcgtcgtc gatctcgcgg gcgagccgtt gcgccccgcg ctctccggct
      421 atccggacgt attgaagatc agccacgaag agttactcgc ggacaagtgg gcgcgcgacg
      481 actcacctgc cgagctcgca cgcgcgatcg ctgcgctcca ggcggccggt gcacgcgatg
      541 tcatcgtctc gcgcggtgcg aacccgtcgc tggcgaacgt cgacggcgag ctggtcgaga
      601 tcatcgcgcc ccgcatgcac gaggtcgatc atcgcggtgc gggcgactcg atgacggccg
      661 ggctcgcggt caacctcgcg cgcggcaagt cgttgcgcga cgaggggctc gcgctggcgg
      721 cggccgccgg cgcgatgaac gtcacccgcc acggtctcgc gtcgggtcac gccgagatga
      781 tcgagcagct cgcgcagcgg gtcgcgatcc ggccggtcag atggcgatga acacgagcgc
      841 gaccgcggcc agcgcgaggc ccgcgagtcg ctgccgcccg atcggttcgt gcagcacgaa
      901 gcgggcgagc acgacggtgg acgccgggta gagcgacgcg accggcgcga ccagcgacag
      961 catctcgccg cgcaccgcga acagcagcag gccgttggct gtggtgtcga gcacgccggc
     1021 cgcgaccgcg acgggccgtt cggagcgatc ggggagccgg ggccggcgcg cggcgaggat
     1081 gaaggtgacg acgagcgtcg ccgcggtgag gcgtgcgatc accaccggcc agaagccgct
     1141 cgcctcgccg gtctcgccga agaagatgaa cgacgtgccg aacccgagcc cggcgccgat
     1201 cgcgagggca agcgcgctgc ggtcgttccc cgtgctcttg tgatcgggtt cggccgcgac
     1261 gatcgcgacc gcaatgatcg cgagcaacac gccgaagagc gcgagtgccg acggatcttc
     1321 gccggtgccc aacccgtagc tgatggggag cagcgcgctc gtcactgcgc tgaccggcgc
     1381 gacgacgccc atgcgaccgg ttgccaggcc gcggtacagc aacacgacgc cgagcacttg
     1441 gcccgcgccc gaaccgatgc tgaggccgat gtcgcgcccc gtgacctcgt cccacccgac
     1501 gaggaacacg atgaccaacg agatgacgaa cccgatcgac tgcgtcatgg cgacgacgga
     1561 atcggaagtg gagcgtttcg acgcgaagcc gccgaggaag tcggaggcgc cgtacgtgat
     1621 cgcgacgcag agacccagca gcacggccat agccggagca atctaggcgt cgtgaccggc
     1681 cgcgaagcac cgtggcgggc cgggcactca cggggttaac gtcctctcgt gcgtatcttc
     1741 gtgtcggtcg acatggaagg catcagtggc gtgacccgct gggcggacgt cgtcaaaagc
     1801 gggcaggact acgagcgggc gcgccgctgg ctcaccgccg acgtgaacgc ggcggtcgcg
     1861 ggcgcccggg cggccggcgc gaccgatttc gtcgtcgagg agaaccacgg caccgagatg
     1921 ctgtgcaacg tgctcctcga cgagatcgac cccgaggtcg aggtcgtgcg cggccaaccg
     1981 cgcggcggtg cgaccacgat gtccgcgctc gacgactcgt tcgacgccgt gttcctcgtc
     2041 gggcaccacg caagctcgag cgactatccg ggtgtgtgcg cacacacgat cagtcactcg
     2101 cactacaagg aagtgcgact cgacggccgg ccgctcaccg aaggcgagat gttcgcgctc
     2161 gcagccgcgc agcgcggcgt gccgacggcg ttgatcaccg gtgacgacgt cgtgaccgcg
     2221 atcctccaga aggtgtgccc cggtatcgag atggccgtcg tgaagcgcgc gctgtctcgg
     2281 gaagcggccg cgatcatccc gccggtgcgt gcccaacgga tcatccacga cgccgcccgg
     2341 cgcgccgtcg aacggttgcg cgcgggcgag atcgacgcgc ccgagccgca cccgccgttc
     2401 gcgatcgagg tcgagctacg cgagtcgatc agcaaggaca tccaggcgac gatcgcgcaa
     2461 cgcttccccg agttcacggt gaacgacgac cgcgtgttct cgttcgcggt cgacgagatg
     2521 gccgtcggct tccgcatggc cgcgatcgtg caggcgctcg cggagacgcc ggagcgagtc
     2581 cgtagctact gagcgaccga gcgaccgaga tcctggtcga tgggcttggt gcgcacccag
     2641 acctgcgtgc gcccgctctc acccggtgca gtcggcgcgt tcacttcgat cttcagcagt
     2701 tcgtagccga gccgctgcgg gatcgcggcg ctgcggacgt tcgcttcgtc ggtgtacacg
     2761 agagaccgcc tgacgccgcg gacgcgcagc gcgacatcgg tcagcgccgc ggccgcgcgg
     2821 gttgcatgcc cctgaccggt gacgtctgcc gcgagccagt agccgatctc gagcgtgccg
     2881 cgaccgcgtc gcgtcatgag accgaacgat ccgcggaacg tcgtgtcgtc gcccggcacg
     2941 aacaacccgt actggtgctc ctcacgcgtg tgccagcgct cgaccgcgcc ccgcagtcgt
     3001 tcgcgctgga acgcggcgtc gcacgcggtc tcgtcggccc aggccatcca cggcttcagg
     3061 tgctccaggt tggcgcgcac tgcggcggcc acggccggcg cgtcgtctac gcccaccatg
     3121 cgcaggtagg cgccggtgag ctcgatgcgc tcgggcggcg caggcacgcg gagcagttgc
     3181 accatatgca agttatcgac gtcgtacgac cccgaatggt tgccccccga gctcatgaag
     3241 cggcggcggt cgtggccgcc cgggcggccg ccacccagcg ggcaaacgcc ccctcgcggc
     3301 tcgcgagctc gtcgggcgcg ccgtcctcga cgatgcgtcc gtgctccagg acgatgaccc
     3361 gatcggcccg cagcgcggtg ccgacccggt gcgcgacgac gatcgacgtg cgttggtcga
     3421 ggaggccgac gagcgcgtgt tcgaccaacg cgtccgtcgc cggatcgagg ttcgaggttg
     3481 cctcgtcgag gacgatcacc gcgggatcgg ccacgaacgc acgcgcgagc gcgacgagct
     3541 gtcgttgccc cgacgacaac gacaggccgc ggttcgcgat gcgggcgtcg agcccgccgg
     3601 ggatgcgccc gagtacgtcg gcgagtccga gtcgttcaca cgccgcgagc agctcggcgc
     3661 gggtcgcggt ggggtggccg agcgcgatgt tgtcggcgat cgtgccgtcg aagaggaagc
     3721 cctcttgcgg cacgaccatc acgtcgcgcc gcagcggcgc gtgtttgacg tcgcgcaggt
     3781 cttgtccgtc gatgacgacg cgccccgcgg tcgggtcgta gaagcggccg atcaacttgg
     3841 ccagcgtcga cttgcccgcg ccgctcgtgc cgacgaccgc gatgcgttcg ccgggctcga
     3901 ggtggatgtc gagatcggcg acggcgggcg gcgcgtcggg cgcgtaccgg aacgtgacgt
     3961 cgtcgacgtg tacctcaccg gcaccgcgcc ggaactcgcg cgcaccgtcc tgatcgcgca
     4021 gcatcggttg gcgatcgagg aactcgaacg tccgggagta cgccgcgctg gcggcctgca
     4081 tcaacgagtt cagctcgatg aactgctgga tcggctcgaa caaccgcgtg aggtacaacg
     4141 tgaacgcgat cagcgtgccg acctcgatct cgccccggtc ggcgaggacc gcgccgtagc
     4201 cgatcacgat cgccagtgcc accggttgca tgaactcgat gacggcgtag tagatcgacg
     4261 tcacacgacc ggcttcgatc tttgcgtcga gcgcggcgtc gttggcgctg gcgaactcgc
     4321 gctgttgctg cttctcgatc gagtaggcct gcacgacgcg catgccgatg agcgcttcgt
     4381 tgacgtggtt gaggaggtcg gcgatcgact cgcggtagcc gacctgcacg ccgaacgcac
     4441 gcggccggta ccagcgaccg gcgaggtaca gcaccggcac gatcacgagg accgccaacg
     4501 cgagctgcca tgactgcgtg aacatgatga gtgcgctgag cgtgagcaac aagatgccgc
     4561 gcagcagcaa cgagaggccg tactgcagga actgctgcag gctctcgacg tcggacgtca
     4621 ggcgcgacac caccttgccc gccggttcgg tggagaagta gtcgaggtcg agctcgtgca
     4681 ggtggccgag cagacggctg cgcaggtcgg cgagatagcg ctcggcgaag cgacccatcg
     4741 cgcgcagttc gctgtaggag ccgagcacct gcccgacgat gagcgtggcc atcaccgcga
     4801 cgcagccgac gatgatgccg gtgttgcggt cgacgacgcc ttggttgatc gcgtacgcga
     4861 cgacgagggg cgagaggacc tggcaggtga cgccgacgac gatgccgacg agcgcgccga
     4921 tgccatcgcg gcgaaatggc gagaactcac gcaggatgcg ccgcagcgac ggccgcttca
     4981 cgacgtcagc tccttcgtct ccagcgcgag tgcccttcgg tactcgggta gtgcgaggag
     5041 ctcgtcgtgc gtgccgtcgg cgacgacccg cccctcgtgc agcaggacga cgcgctcggc
     5101 caggctgatc gtctcgaccc ggtgggcgat gacgagcgtc gtgcgcccct ccatcgcgtg
     5161 cagcaggccg cggcggatct cggcctcggt cggcgggtcg acggcact
//
LOCUS       DASUGA010000259         3424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1126960, whole genome shotgun sequence.
ACCESSION   DASUGA010000259 DASUGA010000000
VERSION     DASUGA010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126960"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VFX21_05105"
     CDS             complement(<1..580)
                     /locus_tag="VFX21_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095366.1"
                     /translation="MTDAFPITAETARGQPLIDDLAVFSPRARGLSAIDDLMLPTEPP
                     IRRPGSRGSTAAETPETRAEMTTSERGPSIDDSLLGISSVDGVLASFGEETNSVPEAL
                     PFEIDSGPVVADPDELVPVRPDLASLQVVVYEDSAQLATAQNAIVAAGHVVAQGGSGR
                     AGLSRVLAAIHGGGIDVVLVALPGGQPIIDAAL"
     gene            973..1890
                     /locus_tag="VFX21_05110"
     CDS             973..1890
                     /locus_tag="VFX21_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095367.1"
                     /translation="MATTTASVPSVATMVTITAPVPTAAVTAVAATTATTCAPLAATA
                     AAVTMATTCVPVAVTAAAATTERMTGPVAVTAAAGTTERMTALAALTAAAATMMTTTA
                     PATMTTAGAAASGVVAEAVWFAARRQTKLHREWATDATEPWGSATDAMEPWGSATDAM
                     MATARAMTATDARAEPQATTATDATDARARPRATTATDARAARQATMATDALMARPLP
                     SRQVRKWLQGSVTDATTKAIAATTETAAEPATRVRRVAAHRACVPTSCAAVPATEFSS
                     GVVPCGPTCRRCARCSSPARRPRRCARCS"
     gene            complement(2176..>3424)
                     /gene="xseA"
                     /locus_tag="VFX21_05115"
     CDS             complement(2176..>3424)
                     /gene="xseA"
                     /locus_tag="VFX21_05115"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF014640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX5095368.1"
                     /translation="EVTQATKPSSGHLYFALRDKDSVLSAVMWSRDLGRMKFRIEPGQ
                     RLRVRGRLGVYDRDGKMQLYADFAEPAGLGAEALALEQLKHKLAAEGMFAAEKKRPLP
                     RFPRRIGVVTSKNGAAVHDIIRTVQRRLPTPILIADAAVQGETAPHQIVQGMAMVVRA
                     GVDVVIVGRGGGAVTDLTAFNHERVVRTISRCPVPVISAVGHEVDLSLSDLAADARAS
                     TPTAAAELAVPDGAALVDVLRKERRRLDREIQHVVARAHQDLDRNTIALHARGERALH
                     ATRAQLHDLHRRLAGLHPRARILAHRAALADLERRLAGLHPAPRIARATHELSALISR
                     RDAAMHDILDERRSELAQLGAQMAALSPLAVLDRGYAMVRAGDVLVRDAAQVAKGERL
                     SVRLAKGALDVIVDKIQKSGEGE"
BASE COUNT          514 a         1126 c         1320 g          464 t
ORIGIN      
        1 cgagcgccgc gtcgatgatc ggctggccgc ccggcaacgc gacgaggacg acgtcgatgc
       61 cgccgccgtg aatcgcagca agcacgcgcg agaggccagc gcgaccgctg ccgccctgcg
      121 cgacgacgtg acctgccgcg acgatcgcgt tctgtgcggt cgcgagttgc gccgagtctt
      181 cgtagaccac gacctgtagc gatgcgaggt cgggacgcac gggcaccagc tcgtcgggat
      241 cggcgaccac cgggccggag tcgatctcga acggcagcgc ctcgggaaca gagttcgtct
      301 cctcgccgaa cgacgcgagc acgccgtcga cagacgagat cccgagcaac gagtcgtcga
      361 tcgacgggcc gcgctcggag gtcgtcatct cggcgcgcgt ctccggcgtc tccgccgccg
      421 tcgagccccg cgatccggga cggcggatcg gcggctcggt cggcaacatc aggtcgtcga
      481 tcgccgacaa cccacgcgcg cgcggcgaga acacggccag gtcgtcgatc aacggctgcc
      541 cgcgagcagt ctctgcggtg atcggaaacg catcggtcac cgagtacgcg cgacccgcgt
      601 cggacgatcc ctcatcgaag gaggacgcgc cctcatcgaa ggaggacgcg ccgcggtcct
      661 cggagaccga gaggtcgtcg tacgccgacg gtacgcgatg gtgttcgagc ggtcgctcgc
      721 tgaacggcga cgatggcggt cgcgcgggag aaggcgttgg acgtgtcggc gtcgccgatg
      781 tcgagggcgt cgcggccgat gggcgcgtcg gtatcgacga cgaggacgaa accgacgtcg
      841 acatgggccg cgtcggcgtc gccgtcggcg tcggcgtcga tgaggtcgac ggtcccgatg
      901 ggcgcgtcgg catcgacgac gtcgagggcg tcggagccga tgggcgcgtc agcatcgacg
      961 acgaggaggc ggatggcgac gacgaccgca tcggtgccaa gtgtggcgac gatggtgacg
     1021 ataaccgccc cggtgccgac ggcggcggtg acagcggtgg cggcgacgac ggcgacgacg
     1081 tgcgccccgc tggcggcgac agcggcggcg gtgacgatgg cgacgacgtg cgtcccggtg
     1141 gcggtgacag cggcggcggc gacgacggag aggatgaccg gtccggtggc ggtgacagcg
     1201 gcggcgggga cgacggagag gatgaccgcc ctggcggctc tgacagcagc ggcggcgacg
     1261 atgatgacga cgaccgcccc tgcgacgatg acgacggcgg gggcggcggc gtcgggcgtg
     1321 gtggcggagg cggtgtggtt cgcggcgcgc cggcagacga agctccaccg ggaatgggcg
     1381 acggacgcga cggagccgtg gggatcggcg acggacgcga tggagccgtg gggatcggcg
     1441 acggacgcga tgatggcgac ggcgcgagcg atgacggcga cggacgcgag ggcggagccg
     1501 caagcgacga cggcgacgga cgcgacggac gcgagggcga ggccgcgagc gacgacggcg
     1561 acggacgcga gggcggcgag gcaagcgacg atggcgacgg acgcgctgat ggcgaggccg
     1621 ctgccgagcc gccaggtgag gaagtggctc cagggatcgg tgacggacgc gacgacgaag
     1681 gcgattgccg cgacgacgga gactgccgcg gaaccggcga cgcgggtccg gagggtggcg
     1741 gcgcatcgag cgtgtgtacc gacgtcgtgc gcggcggtgc cggcaacgga attctcgagc
     1801 ggcgttgtcc cgtgcgggcc gacgtgtcgt cgttgcgcgc ggtgctcgtc acccgctcgt
     1861 cgtcctcgtc gttgcgcgcg gtgctcgtag ggagctcgtc ttccgcgtcg tcggggatga
     1921 tgtcctcgtg cgatggcgcg cgggtctgcg tgcgcggctc gtcgtcgtcg gccatctcct
     1981 cggtgggagg tgccacggtc tcgtcggtcc gcgcgaccat gtcgtcgagt gcagcctcgg
     2041 cagcgtcggc gatgtcgctc ggcaaccggc ccttcgcctt gggccgcggc agctgcacgc
     2101 gctcgaatgg atcggtctcg ccaccgtcgc cgcggcggcc accgcgcgtt ccaccccggc
     2161 gccccttcga gcggctcact cgccctcccc ggacttctgg atcttgtcga cgatgacgtc
     2221 gagcgcgccc ttcgcgagcc gcacgctgag ccgttcaccc ttggcgacct gcgcggcgtc
     2281 gcgcacgagc acgtcgcccg cacgcaccat cgcgtagccg cgatcgagca ccgcgagcgg
     2341 cgacagcgcc gccatctgcg cgccgagttg cgcgagctcg ctgcggcgtt cgtcgaggat
     2401 gtcgtgcatc gcggcgtcgc gacgcgagat cagcgcggac agctcgtgcg tggcgcgggc
     2461 gatgcgcggc gcagggtgaa ggcccgcgag tcggcgctcg agatccgcga gtgctgcgcg
     2521 atgggcgaga atgcgtgcac gcggatgcag cccggcgagc cggcggtgca agtcatgtag
     2581 ctgtgcgcgt gtcgcgtgga gcgcgcgttc accacgtgca tggagcgcga tcgtgttgcg
     2641 atcgagatcc tggtgtgcgc gcgcgaccac gtgctggatc tcgcgatcga ggcgacggcg
     2701 ctccttgcgc agcacgtcga cgagcgcggc accgtcggga accgcgagct cagcggcggc
     2761 cgtcggtgtc gacgcgcgag catcggctgc gagatccgac aacgagaggt cgacctcgtg
     2821 accgacggcg ctgatcaccg gcaccgggca gcgcgagatc gtccgcacga cgcgctcgtg
     2881 attgaacgcc gtgagatcgg tgaccgcgcc gccaccacgg ccgacgatca cgacgtcgac
     2941 gccagcacgc acgaccatcg ccatgccctg cacgatctgg tgcggggccg tctcgccttg
     3001 caccgcggcg tctgcgatca ggatcggcgt cggcagccgg cgctgcacgg tgcgaatgat
     3061 gtcgtgcacc gcggcaccgt tcttcgatgt cacgacgcca atgcggcgcg ggaaccgcgg
     3121 cagcggccgc ttcttctccg cggcgaacat gccctcggcc gcgagcttgt gcttgagctg
     3181 ctcgagcgcg agcgcttcgg cgccgagtcc agccggctct gcgaaatcgg cgtagagctg
     3241 catcttgccg tcgcggtcgt agacaccgag ccggccgcgc acgcgcaacc gctggccggg
     3301 ctcgatccga aatttcatgc gcccgagatc gcggctccac atcacggccg acagcacgct
     3361 gtccttgtcg cgcagtgcga agtagagatg tcccgaggat ggcttcgtag cctgcgtgac
     3421 ttcg
//
LOCUS       DASUGA010000260         2223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_297641, whole genome shotgun sequence.
ACCESSION   DASUGA010000260 DASUGA010000000
VERSION     DASUGA010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297641"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..2196
                     /locus_tag="VFX21_05120"
     CDS             52..2196
                     /locus_tag="VFX21_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX5095369.1"
                     /translation="MNTRRLLLLAGFVALAVAALPHVRPDVDVGPTDIGGVVTGPRGP
                     EAGVWVIAETNDLPTRFVRIVVTDDQGRYLIPDLPKARYSVWVRGYGLVDSPKSTSAP
                     GSTLALTAVAAPDARAAAAYYPAGYWFSLLQVPDRKEFPGTGPEGNGISPNIKSQADF
                     VRTIKSGTCLACHQLGSIGTRRVPDAFKTMPTSFAQWERRVQSGQAGAQMTSAIGALG
                     HQGPLAMFADWTDRIAAGEVPKAPLRPTGLERNVVITEWDWADPKAYLHDVVSTDRRD
                     PRVNANGLLYGSLELSADYLPVLDPARNSISRVPLTVRDSATPIAAGPAAGAPSAYWG
                     DTVLWTSKANVHNPMMDEKGRVWITSAVRPPDNPAVCKAGSSHPSAKLFPLARSGRHL
                     AMYDPTTKKLTHIGTCFSTHHLMFAEDAHRTLWTSGGGPVVGWLDTKKFDETGDEEAS
                     QGWTALVLDANGNGKRDEYTEPNQPIDPTKDHRINNGLYAVSPAPDGSIWGSSLGFPG
                     GIVRLTPGAHPPETALAEYFALPYGNPKAKDPGFSPRGMDVDRNGVAWVALASGHMAS
                     FDRRKCTGPLNGPTATGQHCPEGWTFYTEPLPQLKGLTESGSAEGSYYTWVDQFNVLG
                     LGPNTPINTGNESEALLALKDGKFVVLRVPYPMGFYTKWMDGRIDDPNAGWKGRGLWA
                     TVSTRAPWHMETGKGTTSKVLHFQLRPNPLAN"
BASE COUNT          377 a          795 c          711 g          340 t
ORIGIN      
        1 tccctcattc cctcattccc tcattccctc attcccgcat cccgagggat catgaacaca
       61 cgccgtctgc tccttctcgc cggcttcgtc gcgctcgcgg tcgcggcgtt gccgcacgtt
      121 cgtcccgacg tcgacgtcgg ccccaccgac atcggcggtg tcgtcaccgg tccgcgtggt
      181 cccgaagcgg gcgtgtgggt catcgccgag acgaatgatc tcccgacgcg cttcgtgcgc
      241 atcgtcgtca ccgacgacca gggacggtac ctcatccccg atctgccgaa ggcgcgctac
      301 tccgtgtggg tgcgcggcta tgggctcgtc gactcaccga agtcgacgtc ggcgccgggc
      361 agcacgctcg cgctcaccgc ggtggccgcg ccggacgcgc gtgcggccgc cgcgtactac
      421 ccggccggct actggttctc gctgctccag gtaccggaca ggaaggaatt tcccggcacg
      481 ggccccgagg gaaacggtat ctcgccgaac atcaagtcgc aggccgactt cgtacgcacg
      541 atcaagtcgg ggacgtgcct ggcctgccac cagctcggct cgatcggtac gcgtagggtt
      601 cccgacgcgt tcaagacgat gccgacgagc ttcgcgcagt gggagcgtcg cgtgcagtcc
      661 gggcaggccg gcgcgcagat gacctccgcg atcggcgcgc tcggccacca gggtccgctc
      721 gccatgttcg ccgactggac cgaccgcatc gccgcgggag aggtgccgaa ggcacctctc
      781 cgtccaacag gcctcgagcg taacgtcgtg atcaccgagt gggactgggc cgatccgaag
      841 gcgtacctgc acgacgtcgt ctccaccgat cggcgcgacc cgcgcgtcaa cgcgaacggc
      901 ctgctctacg gatcgctcga gctgagcgcg gattatctcc ccgttctcga tcccgcgcgc
      961 aacagtatca gccgcgtgcc gctcaccgtg cgcgacagcg cgacgcccat cgccgcgggt
     1021 ccggccgccg gcgcgccttc cgcgtactgg ggcgacaccg tgctgtggac gagcaaggcg
     1081 aacgtccaca atccgatgat ggacgagaag gggcgcgtgt ggatcacctc ggccgtgcgc
     1141 cctcccgaca atcctgccgt ctgcaaggcc ggctcgtcgc atccgtcggc gaagctcttc
     1201 ccgctcgcgc gctcggggcg ccatctcgcg atgtacgatc cgacgacgaa gaagctgacg
     1261 cacatcggca cctgcttcag cacgcatcac ctcatgttcg ccgaggatgc gcaccgcacg
     1321 ctgtggacga gcggcggcgg ccccgtcgtc ggctggctcg acacgaagaa gttcgacgag
     1381 acgggcgacg aggaagcgtc gcagggctgg accgcgctcg tcctcgacgc gaacggcaac
     1441 gggaagcgtg acgagtacac ggagccgaac cagccgatcg atccgacgaa ggatcatcgc
     1501 atcaacaacg gcctctacgc cgtgtcgccc gcacccgacg gctcgatctg gggctcgtcg
     1561 ctcggcttcc ccggcggcat cgtccgtctc acgccgggcg cgcatccgcc cgagaccgcg
     1621 ctcgccgaat acttcgcgct tccgtacggc aacccgaagg cgaaggatcc cggcttctca
     1681 ccgcgcggca tggacgtcga ccgcaatggt gtcgcctggg tcgcgctcgc gagcggacac
     1741 atggcgagct tcgaccgccg caagtgcacc ggcccgctca acggccccac cgccaccggc
     1801 cagcactgcc ccgaaggatg gaccttctac accgagccgc tcccgcagct gaagggactg
     1861 acggagtcgg ggagcgccga aggcagctac tacacctggg tcgatcagtt caacgtgctc
     1921 ggactcggcc ccaacacgcc gatcaacacc ggcaatgaat cggaggcact gctcgcgctg
     1981 aaggacggca agttcgtcgt gctgcgcgtg ccgtatccga tgggcttcta cacgaagtgg
     2041 atggacggcc gcatcgacga tccgaacgcc ggctggaagg ggcgcgggct ctgggccacc
     2101 gtgagcacgc gcgcaccctg gcacatggag accggcaagg gcacgacgag caaggtgctg
     2161 cacttccaat tgagaccgaa tccactggcg aactgaaaac gcagttttcg gttcacggtg
     2221 ttc
//
LOCUS       DASUGA010000261         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1678791, whole genome shotgun sequence.
ACCESSION   DASUGA010000261 DASUGA010000000
VERSION     DASUGA010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678791"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFX21_05125"
     CDS             <1..258
                     /locus_tag="VFX21_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018639955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX5095370.1"
                     /translation="AQRKSRGLRDEVAALDGERVDLYRALESLPKRQRQVVMLRYVAD
                     LPEAAVATALHCSVGNVKSNASRGLATLRARLAIGDDEEIS"
     gene            258..1571
                     /locus_tag="VFX21_05130"
     CDS             258..1571
                     /locus_tag="VFX21_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095371.1"
                     /translation="MFEHLDDPHPSGPELTGLEDARVRGRVLRNRRRSTLGGGATLAL
                     VLLIAGASVAWSADDRGRVNVGDGDTPTSAAVDATTTTEPEAEAPWVEHVDETGGYRI
                     LFPVEPTGFHVSTDVSARLELVDDHSYAAEDDGGRYQIRWFDVPAEAATDPAALMQEL
                     AEYWASSSSPGELSGMTETTSFGNPAADFVVTERGAGDDETDVVRHHMIVRGTRVYWM
                     ATSVTAAADGSHERFVGSFSFLEEQTTTTTRPTTDERPPTTTTTTPTVPTSTPATDWS
                     GPRVEMPTAFPAGDYIDPAGRFSTSLPTLGYSRYFFVATWFGGPPVHDYWIASDTGEY
                     LDELRVQDYAPGTIADADGFARRLAKYWCSALDGSFGSEPVTTTEKDRPAVRFRCDHP
                     VSGATSYSWSTIIVDGDRVYWLNTSAFVEQPAEHRHWIDLLQILG"
     gene            complement(1575..>2087)
                     /locus_tag="VFX21_05135"
     CDS             complement(1575..>2087)
                     /locus_tag="VFX21_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095372.1"
                     /translation="KPHDPAVYEHGLVESVEAVLPGEPVDAIGFSLGAATLLRIATED
                     ASRFHRLVLMGIGAHTLGVSTGGPQTISATDDRVLTQLADSDRNDPGALDALRRRPQH
                     PFTDDELARVTVPTLVITSDADGLAGSPQPLVDKLPNVELKLLRGIDHFQTPREFGAI
                     DAAVEFLAKG"
BASE COUNT          314 a          710 c          749 g          314 t
ORIGIN      
        1 gcgcaacgaa agtcgcgtgg cctgcgcgat gaggtggcgg ccctcgatgg tgagcgcgtc
       61 gacctgtacc gggcgttgga gtcgctgccg aaacgtcagc gtcaggtcgt gatgctgcga
      121 tacgtcgcgg acttgcccga agccgcggtc gcgaccgcgc tgcactgctc ggtcgggaac
      181 gtgaagtcga acgcatctcg agggctcgcc acgttgcgcg cacggctcgc gatcggcgac
      241 gacgaggaga tctcctgatg ttcgagcacc tcgacgatcc gcacccgtcc ggccccgagc
      301 tcaccggcct cgaggacgcg cgagtgcggg gccgcgtgct gcgcaatcgc cggcgctcga
      361 cgctcggtgg cggcgcgacg ctcgcgctcg tcctgctcat tgccggtgcc tcggttgcgt
      421 ggtcggccga cgatcgcggt cgggtgaatg tcggcgacgg tgacacaccg accagtgcag
      481 ccgtcgacgc gacgaccacc accgagcccg aggcggaggc gccgtgggtc gagcacgtcg
      541 acgagaccgg cggctaccgc atcttgttcc cggtcgagcc caccgggttc cacgtcagta
      601 ccgacgtctc cgcgcgtctc gagctggtcg acgaccactc gtatgccgcc gaagacgacg
      661 ggggccggta ccagatccgt tggttcgacg tgcccgcgga ggcggcgacc gatccggctg
      721 cgctgatgca ggagctcgcc gagtactggg cgagctcctc gagcccgggc gagctctccg
      781 gcatgaccga gacgacgagc ttcggcaacc cggccgccga cttcgtcgtg accgagcggg
      841 gcgcgggcga cgacgagacc gacgtcgtgc gccaccacat gatcgtgcgc ggcacgcgcg
      901 tgtattggat ggcgacaagc gtgacggcag ccgccgatgg ctcacacgag cgcttcgtcg
      961 gcagcttctc gttcctcgag gagcagacca cgacgacgac gcggcccacg acggacgagc
     1021 ggccgccaac gacgacgacg accacgccga cggtccccac cagtaccccg gcgaccgatt
     1081 ggtccgggcc gcgcgtggag atgcccacag cgttcccggc cggcgactac atcgacccgg
     1141 ccggccggtt cagtacctcg ctaccgacgc tcgggtactc gcggtacttc ttcgtcgcga
     1201 cgtggttcgg tggaccgcct gtgcacgact actggatcgc gtccgacacc ggcgagtacc
     1261 tcgacgagct ccgggtccag gactacgcac ccggcacgat cgccgacgcg gacgggttcg
     1321 cgcggcgcct cgccaagtac tggtgttccg ccctggacgg gtccttcggg agtgagccgg
     1381 tcacgacgac ggagaaggac cgcccggccg tgcgcttccg ttgcgaccac cccgtgagcg
     1441 gcgcgacgtc gtactcgtgg agcacgatca tcgtcgacgg cgaccgcgtg tactggttga
     1501 acacgagcgc cttcgtcgag cagccggccg agcaccggca ctggatcgac ctgctgcaaa
     1561 tcctcggctg agtctcagcc cttcgcgagg aactcgaccg cggcgtcgat tgcaccgaac
     1621 tcgcgcggcg tctggaagtg gtcgatgccg cgcagcagct tcagctccac gttcggcagt
     1681 ttgtcgacga ggggctgtgg cgacccggcc agaccgtcgg cgtcgctggt gatcacgagc
     1741 gtgggcacgg tgacgcgggc gagctcgtcg tcggtgaacg ggtgctgcgg gcggcgccgt
     1801 aaggcgtcga gcgcgccagg atcgttgcgg tcggagtcgg cgagctgggt gaggacgcgg
     1861 tcgtcggtcg cgctgatcgt ctgcgggccg ccggtgctca cgccgagcgt gtgcgcaccg
     1921 atccccatga gcacgaggcg gtgaaagcgc gacgcgtcct cggtcgcgat gcgcaggagt
     1981 gtcgcggcgc cgagcgagaa gccgatcgcg tcgaccggtt cgccgggcag cacggcttcg
     2041 accgactcga ccagcccgtg ttcgtagacc gcggggtcgt gcggttt
//
LOCUS       DASUGA010000262         2341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1421479, whole genome shotgun sequence.
ACCESSION   DASUGA010000262 DASUGA010000000
VERSION     DASUGA010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421479"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1650
                     /gene="ahcY"
                     /locus_tag="VFX21_05140"
     CDS             175..1650
                     /gene="ahcY"
                     /locus_tag="VFX21_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX5095373.1"
                     /translation="MSNTLPARAKTVAEGDYKVADLSFAPFGRKEIQLAEHEMPGLMA
                     LRERYSASQPLAGARITGSLHMTIQTAVLIETLLALGAEVRWASCNIFSTQDHAAAAL
                     AVRGVPVFAWKGETLEEYWWCTEQALRWPAKDGEASQGPNMILDDGGDATLVVHKGVE
                     FERAGAVPDVDPSDPEDWRECLTLLQRVFASDPKAWHTLAAGIKGVTEETTTGVHRLY
                     QMHADGSLLFPAINVNDSVTKSKFDNLYGCRHSLVDGICRATDVMLAGKVAVVCGYGD
                     VGKGCAQALKGQGARVVITEIDPICALQAAMEGYQVLRVEDVVETADLFVTATGNSNV
                     ITADHMARMKHNAIVGNIGHFDNEIDMAALTAFPGIERVNVKPQVDEWKFPADGDRPA
                     HSIIVLAEGRLLNLGCATGHPSFVMSNSFTNQVIAQIELFTHTDFYERRVYTLPKHLD
                     EEVARLHLEKLGVRLTELSEAQSSYLGIPVQGPYKPEHYRY"
     gene            1668..>2341
                     /locus_tag="VFX21_05145"
     CDS             1668..>2341
                     /locus_tag="VFX21_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX5095374.1"
                     /translation="MPDPIPPTVAWRRGRIRLIDQRALPQRLRFVECTTTAELVRAIR
                     ELVVRGAPALGASGAYGVALAAAARSRPAQVRADAARVRDARPTAVNLAHGVDHALSA
                     YEQGGAAAALAAAEAYAAADVAVNRALGAHGAVLVPDDGRVLTHCNAGALACVGYGTA
                     VGVIRAAAEAGKSPHVWVDETRPVLQGARLTAWELDRLGIAHTIVVDGAAASLMARGD
                     VDLVVV"
BASE COUNT          358 a          796 c          843 g          344 t
ORIGIN      
        1 gcagcgttcg tcgtgactgg tcaggtccga gccacgcgcg ggggaaccaa accgggggga
       61 gcacggggtg gtcgtccctg agtggagagc ccactcaagg cgaccacctc gtgcgttatc
      121 gtgtgcaatc gcggcgtcac cagcggcgaa ccgcggcccc tgaggagttc gttgatgtcc
      181 aacacgctgc ccgcccgcgc gaagaccgtt gccgagggcg actacaaggt cgcggacctg
      241 tcgttcgccc cgttcggccg caaggagatc cagctcgccg agcacgagat gcccggcctg
      301 atggcgttgc gcgagcgcta ctcggcgtcg cagccgctcg ctggtgcgcg catcaccggc
      361 tcgttgcaca tgaccatcca gaccgcggtg ctcatcgaga cgctgctcgc gctcggtgcc
      421 gaagtccggt gggcgtcgtg caacatcttc tccacgcagg accacgcggc ggccgcgctc
      481 gcggtgcgcg gtgtccccgt gttcgcgtgg aagggcgaga cgctcgagga gtactggtgg
      541 tgcacggagc aggcgttgcg ctggcccgcc aaggatggtg aggcgagtca gggccccaac
      601 atgatcctcg acgacggcgg tgacgccacg ctcgtggtgc acaagggcgt ggagttcgag
      661 cgcgccggtg ccgtgcccga cgtcgacccg tcggacccgg aggactggcg tgaatgtctc
      721 acgctgctgc agcgggtgtt cgcctccgac ccgaaggcgt ggcacacgct cgcggcgggc
      781 atcaagggcg tcaccgagga gaccacgacc ggtgtgcacc ggctctacca gatgcacgcc
      841 gacggctcgc tgctgttccc cgcgatcaat gtcaacgact ccgtgacgaa gtcgaagttc
      901 gacaacctgt acggctgtcg gcactcactc gtcgacggca tctgccgcgc caccgatgtg
      961 atgctcgcgg gcaaggtcgc ggtggtgtgc ggttatggcg acgtcggcaa gggctgcgcg
     1021 caggcgctga agggccaagg cgcgcgcgtc gtcatcaccg agatcgatcc gatctgcgcg
     1081 ctgcaggcgg cgatggaggg gtaccaggtg ctgcgagtgg aggacgtcgt ggagacggcc
     1141 gacctgttcg tgaccgcgac cgggaactcc aacgtgatca ccgccgacca catggcgcgg
     1201 atgaagcaca acgcgatcgt cggcaacatc ggtcacttcg acaacgagat cgacatggcc
     1261 gcgctcaccg cgttcccggg catcgagcgc gtgaacgtga agccgcaagt cgacgagtgg
     1321 aagttccccg ccgacggcga ccgtccggcg cactcgatca tcgtgctggc ggagggtcgg
     1381 ctcctgaacc tcggctgcgc caccggccac ccgagcttcg tgatgtcgaa cagcttcacc
     1441 aaccaggtga ttgcgcagat cgagctgttc acgcacaccg acttctacga gcgtcgcgtc
     1501 tacacgctgc ccaagcacct cgacgaagag gtcgcgcggc tgcacctcga gaagctcggc
     1561 gtgcggctca ccgagctcag cgaagcgcag tcgagctatc tcggcatccc ggtccagggc
     1621 ccctacaagc ccgagcacta ccggtactga acccgccgca tcgagtcatg cccgatccga
     1681 tcccgccgac cgtcgcatgg cggcggggtc ggatccggct catcgaccag cgcgcgctgc
     1741 cgcagcggct gcgtttcgtc gagtgcacga ccacggcgga gctcgtgcgc gccatccgcg
     1801 agctcgtggt ccgcggcgca cctgcgctcg gcgcgtcagg cgcgtacggg gtggcgctcg
     1861 cggcggccgc gcggtcgcgt ccggcgcagg tgcgcgccga cgcggcgcgt gtgcgcgacg
     1921 cgcgtccgac ggcggtcaac ctcgcgcacg gcgtcgacca tgcgctgtcc gcgtatgagc
     1981 agggtggcgc cgcggccgca ctcgcggcgg ccgaggcgta cgcggcggcc gacgtcgcgg
     2041 tgaaccgcgc gctcggtgcg cacggcgccg tgctcgtgcc cgacgacggg cgggtcctca
     2101 cgcactgcaa cgcaggggcg ctcgcgtgcg tcggctacgg cacggcggtc ggcgtcatcc
     2161 gggccgcggc cgaggcgggg aagtctccgc acgtgtgggt cgacgagacg cggccggtgc
     2221 tccaaggcgc gcggctgacc gcgtgggagc tcgatcggct cgggatcgcg cacaccatcg
     2281 tggtcgacgg cgcggccgcg tcgttgatgg cgcgcggcga cgtcgacctc gtggtcgtgg
     2341 a
//
LOCUS       DASUGA010000263         6753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1817805, whole genome shotgun sequence.
ACCESSION   DASUGA010000263 DASUGA010000000
VERSION     DASUGA010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6753
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1817805"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VFX21_05150"
     CDS             complement(<1..391)
                     /locus_tag="VFX21_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018570980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEX5095375.1"
                     /translation="MVSNDIAARLADLARPTVTFASDNAATVHPAVMDALAAANHGHA
                     IAYGDDVWTGRLRESLRTIFGAPVEALPVWGGTGANIVGLAGMVQASDAIVCPDSAHI
                     HVDEAGAPERMAGVKLLPVPTVDGKLRP"
     gene            complement(598..3042)
                     /locus_tag="VFX21_05155"
     CDS             complement(598..3042)
                     /locus_tag="VFX21_05155"
                     /inference="COORDINATES: protein motif:HMM:NF021446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HEX5095376.1"
                     /translation="MTTTMTPEPNAPSSGRRAGATWVAATGVFLLLAAAAVFVATRWN
                     DIPQAAKLAVLVGITGACVLAGDRLRATLPSTGNALYHLGAFLIPIDIAALGVRAHLE
                     WPELLLFDSVVTTAALTLLAWRSESVVLAAGASASVIAIAAGVGAVTGVPAPLVLVAL
                     AAVATWFAVTERHAFVWAIVAGVAPIVTLALQDIVTGRGVAQQLGVLAVHPWYASAAT
                     GALAAFVIVRGARRRGEPALALFALAALAAHGIAAWDATTVPEVVQILMVPSIFLAVE
                     LTALAARRDAFWAQPAARLGQVVELPAALMTLYATGIGLLAVVTFEIQSDARVAVAAG
                     LAALAWLVAGVRRDPRDRSLVASIVEGPQWSSATAGSVAMVSTAVVAATMHPVAVAIT
                     ALGLALVVLAGTQRLRSLIAFLACGYATVAGTGDHRFTLVLGAIAASVAAVGIVRSCV
                     TEDVVLGLVTGLATGGLGELVGAEHAPRLFAALLWVSTCWLLAYACDTREQGSGDVAR
                     LAAVMSLLVSVPWSGVDALVPTVFLLLALVGDGVTRNRQELLVAAVAPLLAIEFVLAD
                     LAGLDIASTGLALTLAASVWLGVAALAREPWRRPLLVAGGASVVAGLIAASASPDTFG
                     PALFVAGGIAVAYGLLLDVVPVVHIGALAATLGAWTTFAAHDLQYAELYAAPVALHLL
                     GTGFAARRKTEHRPNSWVAYAPGLALLTAAAFVERLGGGSANHSLIAGAIAVIAVILG
                     GTYRLAAPLVVGTATLVLVVGREAFASTAGIPTWAWLAAGGVTLIGAAVSMERHDVGP
                     VEAGRRVVDVFAAHFE"
     gene            complement(3039..4415)
                     /locus_tag="VFX21_05160"
     CDS             complement(3039..4415)
                     /locus_tag="VFX21_05160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX5095377.1"
                     /translation="MTTTTAPHRPSASDLAAATPAGRDRYVDFLRIASIAVVVAGHWL
                     MSVVTWHDGELETGNVLGMVPWLWLATWVLQVMPIFFFVGGFANVVTLDALHRKGQGT
                     PEFLAGRVQRLLRPVVTLFAVWIPLSVVILAAGVDHDAFGKATRIVCQPLWFVGAYLM
                     VTAFAPVMRTLHSRFRTNVLAVLAIATITVDIVRFNGSAGVGYFNLLFVWLFAQQLGF
                     FYADGSLLRLSRTRLAAMAGAAVTALVALTTFGPYPNSMVGLPGAKVSNMTPPTTCLL
                     VLTVFQVALVMLARPAVSRWLERRRVWTAVVAGNGVIMTIFLWHLTALLVTIGALYPI
                     GFPQPEGGSAMWWATRPLLFGAALVPLTLFVALLGRFERPKPTTYDCVDHATVPAAAA
                     VAATALGIYGMAASNLADVFTGHPANVVVLQLTFLQSVGLGFLGWVLLRTTVRPQRSR
                     LFPKGIAR"
     gene            complement(4607..5230)
                     /locus_tag="VFX21_05165"
     CDS             complement(4607..5230)
                     /locus_tag="VFX21_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5095378.1"
                     /translation="MAAVRVVLADDHQIWRSGVKTDLGEAFFVAAEAGDADEAIAAIR
                     REKPELVVCDLHMPNGGGIKVAKECGEETRIVMLTVSEQERDLLDAVAAGAQGYLVKS
                     TPGPELRTALLRAAKGEPVFSPHLASLVLGEFRRMSKDATGANPLSDREREVLQQVAR
                     GYSYKDIGERLFISPKTVENHVRNILQKLHLNRKQELIRYAVEHGIE"
     gene            complement(5218..6330)
                     /locus_tag="VFX21_05170"
     CDS             complement(5218..6330)
                     /locus_tag="VFX21_05170"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5095379.1"
                     /translation="MAVFRWGAWVWMAVVMLLNRADLGRPVLAWSLLGMTLAWTAAAT
                     VLVERRPEWLERPAAIGLELSLAMLLGIGGGIVYEHTNSVGEAFSSVRTIGFAWPLVG
                     LMSAGVVYGGAAGLFAGLLVALPRWFAPIVNGVRFDDYETAHWFSLVSTTLLYALAGG
                     VAGYMASLLRNSESQLRQAESVIAASRAREHVARELHDGVLQTLAIIERRSDDPDLAR
                     MAREQERDLREFLFGAGPSADRGGAKDVGPRIRAAAARYEDAFGGRVDVVLAPDLPHL
                     DAARADALAGAVGEALVNAGKHGHAQRVTVYAEPGDGGGVSCSVHDDGDGFDVANAQE
                     RVGLSRSIRSRMQEVGGNVEIESRPGAGTEVRLWLP"
     gene            6387..>6753
                     /locus_tag="VFX21_05175"
     CDS             6387..>6753
                     /locus_tag="VFX21_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5095380.1"
                     /translation="MADQAQFAELTMPFMSALYAAALRMTRNPSDAEDLVQETYLRAY
                     RGFEGFREGTNLKAWLYKILTNTFINQYRAKKRRPDEVDLDDTEDFYLFRRLGGLENV
                     DATRTAETEVLDAIPDAEVK"
BASE COUNT         1169 a         2422 c         2308 g          854 t
ORIGIN      
        1 cgggccgcag cttgccgtcg accgtgggga ccgggagcag cttcacaccg gccatgcgct
       61 cgggcgcgcc ggcttcgtcg acgtggatgt gtgcgctgtc ggggcacacg atcgcatcgc
      121 tggcctgcac catgccggcc aagccgacga tgttcgcgcc cgtcccgccc cagaccggga
      181 gtgcttcgac cggggcgccg aagatcgtgc gcaacgactc gcgcaggcgt ccggtccaca
      241 cgtcgtcgcc gtacgcgatc gcgtggccgt gattggcggc ggcgagcgcg tccatcaccg
      301 cgggatgcac tgttgccgcg ttgtcgctgg cgaacgtcac ggtcggacgc gccagatcgg
      361 caagtcgcgc cgcgatgtcg ttcgatacca cggagcgacg ctaccaaccg cctttctctg
      421 tcgctctcga cctgctcgcg tgaggcgggc cggattgctg gccggcctcg agactccagc
      481 gagtgtccga gaaggtccag ggggcgcttc gcgcatcgcc aggacacaaa ggacaccgcc
      541 ccctggggcg ctgtgttccg tcgcgtcggc aacactgcga cgcgcatctg acgctggtca
      601 ctcgaagtgc gcggcgaaca cgtcgacgac ccggcgcccg gcctcgaccg ggccgacatc
      661 atgccgctcc atcgacaccg ccgcgccgat caacgtcacg ccacctgccg ccagccatgc
      721 ccacgtcggg atgccggcgg tcgaggcgaa cgcttcacgg ccgaccacga gcacgagcgt
      781 cgcggtgccg acgaccaacg gcgccgcgag ccggtacgtg ccgccgagga tgaccgcgat
      841 cacggcgatc gcaccggcga tcagcgagtg gttcgcgctg ccgccgccga gccgttccac
      901 gaacgcggct gccgtcaaca gtgcgagccc cggtgcgtac gccacccacg agttcgggcg
      961 gtgctcggtc ttgcggcgcg ccgcgaagcc cgtgccgagc aagtgcaacg cgacgggcgc
     1021 ggcgtagagc tcggcgtact gcagatcgtg cgcggcgaac gtcgtccacg cacccagggt
     1081 cgccgcgagc gcaccgatgt gcacgacggg tacgacgtcg agcagcaagc cgtacgcaac
     1141 cgcgatgccg cccgcgacga acagcgcggg accgaacgtg tcggggctcg ccgatgccgc
     1201 gatcaacccg gcgacgacgc ttgcgccacc ggcgacgagc aacggccgcc gccacggttc
     1261 gcgtgcgagc gcggccaccc cgagccacac cgacgccgcc aacgtcaggg cgagacccgt
     1321 cgacgcgatg tcgagaccgg ccaggtcggc cagcacgaac tcgatcgcga gcagcggcgc
     1381 gaccgcggcc accaacagct cttgccggtt gcgcgtcacg ccgtcgccga cgagcgcgag
     1441 caacagaaag acagtgggca ccaacgcatc gacacctgac cacggcacgc tgacgagcaa
     1501 cgacatcacc gccgcgaggc gcgccacgtc accggaaccc tgttcgcgcg tgtcgcacgc
     1561 gtacgcgagc aaccaacatg tcgagaccca cagcaaggcg gcgaacaacc gcggcgcgtg
     1621 ctccgcgccc accaactcgc ccaggccgcc ggtcgcgaga cccgtgacga gtccgaggac
     1681 gacatcctcg gtcacacagg accgcacgat ccccacggct gccaccgagg ccgcgatcgc
     1741 gccgagcacg agcgtgaacc ggtggtcgcc cgtgcccgca accgttgcgt agccgcacgc
     1801 caggaacgca atcagcgatc gcagtcgttg cgtaccggcg agcacgacca gcgcgagccc
     1861 gagcgcagtg atcgccacgg cgacgggatg catcgtcgcc gcgacgaccg cggtgctcac
     1921 catggcgaca ctgcccgcag tcgccgagct ccactgcgga ccttcgacga tcgacgcgac
     1981 gagcgaacga tcccgcgggt cccgccggac gccggcgacg agccatgcga gcgcggcgag
     2041 cccggccgcg acggcgacgc gcgcgtcgga ctggatctcg aacgtgacga ccgcgaggag
     2101 cccgatgccg gttgcgtaca acgtcatcag cgccgccggt agctccacga cctgtccgag
     2161 tcgcgcggcc ggctgggccc agaacgcatc acggcgtgcg gccaacgcgg tcagctcgac
     2221 ggccaagaag atcgacggga ccatcaggat ctgcacgacc tcggggacgg tcgtcgcgtc
     2281 ccatgcggcg atcccgtgcg cggcgagtgc cgccagtgcg aacagcgcga gcgccggttc
     2341 accgcgccgg cgggcacccc ggacgatcac gaacgccgcc agggcgcccg tcgccgccga
     2401 cgcgtaccac gggtggaccg ccagcacgcc gagctgttgg gcgacgccgc ggccggtgac
     2461 gatgtcctgg agcgccaacg tgacgatcgg cgcaaccccg gcaacgatcg cccagacgaa
     2521 cgcatgccgt tcggtgacgg cgaaccacgt cgccacggcg gccaacgcca cgagcaccaa
     2581 cggcgccggc acgccggtga ccgcaccgac acccgcggcg atcgcgatca ccgacgcgga
     2641 cgcgccggcc gcgagcacga cggactcgct gcgccacgcg agcagcgtca gcgcggcggt
     2701 cgtcaccacg ctgtcgaaca acaacagctc cggccactcg agatgggcgc gcaccccgag
     2761 cgcggcgatg tcgatcggga tcaggaaggc gcccaggtgg tacagcgcgt tgccggtcga
     2821 ggggagcgtg gcgcgcaacc ggtcaccggc gaggacgcac gcgccggtga tgccgacgag
     2881 caccgcgagt ttcgcggcct gcgggatgtc gttccagcgg gtcgcgacga acaccgccgc
     2941 cgcggccagc aacaagaaca cgcccgtcgc cgcaacccag gtcgcgccgg cgcggcggcc
     3001 ggacgacggc gcgttcggtt cgggtgtcat cgtggttgtc atcgtgcgat gcctttcggg
     3061 aagaggcgcg agcgctgcgg ccgcacagtc gtgcgcagca gcacccagcc caggaagccg
     3121 agcccgacgc tttggaggaa cgtgagctgg aggacgacga cgttcgcggg gtgacccgtg
     3181 aacacgtcgg cgaggttcga cgccgccatg ccgtagatgc cgagtgcggt tgccgcgacg
     3241 gcggccgccg ccggcaccgt tgcgtgatcg acgcagtcgt acgtcgtcgg cttggggcgc
     3301 tcgaagcgac cgagcagtgc gacgaagagc gtgagcggga cgagcgcggc gccgaacaac
     3361 aacggccgcg tcgcccacca catcgcgctg ccaccctcgg gctgcgggaa gccgatcggg
     3421 tacagcgcgc cgattgtcac cagcagcgcg gtgaggtgcc acaggaagat cgtcatgatc
     3481 acgccgttgc cggcaacgac cgccgtccac acccggcgcc gttcgagcca acggctcacc
     3541 gcgggacgag cgagcatgac gagcgcgacc tggaacacgg tgagcacgag caagcacgtc
     3601 gtcggcggcg tcatgttcga gaccttggcg ccgggcaggc cgaccatgct gttcgggtac
     3661 gggccgaacg tcgtcagtgc gacgagcgca gtcactgccg caccggccat cgccgcgagc
     3721 cgcgtgcgcg acaggcgcag caacgaaccg tcggcgtaga agaagccgag ctgttgtgcg
     3781 aacagccaca cgaacagcag gttgaagtaa ccgacaccgg cggaaccgtt gaatcggacg
     3841 atgtcgaccg tgatcgttgc gatcgccaag accgcgagca cgttggtgcg gaagcgcgag
     3901 tgcaacgtgc gcatgaccgg cgcgaacgcg gtgaccatca agtacgcgcc gacgaaccac
     3961 agcggctggc acacgatgcg cgtggccttg ccgaacgcgt catggtcgac gccggcggcg
     4021 aggatcacga ccgagagcgg gatccacacc gcgaacagcg tgacgaccgg ccgcaatagc
     4081 cgctgcacgc gccccgcgag gaactcgggc gtgccctgcc ccttacggtg cagcgcgtcg
     4141 agcgtcacca cgttcgcgaa gccgccgacg aagaagaaga tcggcatcac ctgcagcacc
     4201 caggtggcca gccacagcca cgggaccatg ccgagcacgt tgcccgtctc gagctcgccg
     4261 tcgtgccacg tcaccacgga catcagccag tggccggcga cgacgaccgc gatcgacgcg
     4321 atgcgcaaga agtcgacgta gcgatcccgg ccggcgggcg tggccgcggc caggtccgag
     4381 gccgaagggc ggtgcggggc agtcgtcgtg gtcatgagac ccatgttcag gccgcgcgcc
     4441 cggtgctccc accgggtgct tcccccatat ggatgtgggg ggcccggccg gggttgtgct
     4501 cacggaccga aaatcagggc tccatccggc cgaccggccg gtgacggaga acccggggga
     4561 cgggagcggc aatggactgc cgctgccgcc ccgtcggcgt gccgcgctac tcgatgccgt
     4621 gctcgacggc gtaccggatc agctcttgct tccggttgag gtgcagcttc tgcaagatgt
     4681 tgcggacgtg gttctcgacc gtcttgggtg agatgaacag ccgctcaccg atgtccttgt
     4741 acgagtagcc gcgcgcgacc tgctggagga cttcgcgttc gcggtcggag agcgggttcg
     4801 ctccggtggc gtccttggac atccggcgga actcgcccag cacgagcgac gcgagatgcg
     4861 gcgagaacac cggctcgccc ttcgcggcgc gcaacagcgc ggtgcgcagc tcgggtccgg
     4921 gcgtgctctt cacgaggtag ccctgcgcgc cggccgcgac cgcgtcgagg aggtcacgct
     4981 cttgctcgga gacggtgagc atcacgatgc gcgtctcctc gccgcattcc ttggcgacct
     5041 tgatgccccc gccgttcggc atgtgcaagt cgcacacgac gagctccggc ttctcgcgcc
     5101 ggatcgcggc gatggcctcg tcggcgtcgc ccgcctcggc cgcgacgaag aacgcttcgc
     5161 ccaggtcggt cttcacgccg ctccgccaga tctgatggtc gtcggccaac accactctta
     5221 cggcagccac aaccgcacct ccgtccccgc acccgggcgg ctctcgatct cgacgttgcc
     5281 gccgacttct tgcatgcggc tacgtatcga acgcgacagc ccgacgcgct cctgcgcgtt
     5341 cgcgacgtcg aagccgtcgc cgtcgtcgtg caccgaacac gacacgccgc cgccgtcgcc
     5401 cggttctgcg tacacggtga cgcgttgcgc gtgcccgtgc ttgcccgcgt tcacgagcgc
     5461 ttcgccgact gcgcccgcga gcgcgtcggc gcgcgcggcg tcgaggtgcg ggagatcggg
     5521 tgcgagcacg acgtctactc ggccgccgaa ggcgtcttcg tagcgcgcgg ccgcggctcg
     5581 gatccgcggc ccgacgtcct tggcgccgcc ccgatcggca gacgggccgg cgccgaagag
     5641 gaactcccgc aggtcgcgtt cctgctcccg cgccatgcgg gcgaggtcgg ggtcgtcgga
     5701 gcggcgctcg atgatcgcga gcgtctggag cacgccgtcg tgcagctcgc gcgcgacgtg
     5761 ctcacgggcg cgagatgcgg cgatcacgct ttcggcctgt cgcagttggc tctcggaatt
     5821 gcgcagcagt gacgccatat atcccgcgac gccgccggcg agcgcgtaga gcaaggtcgt
     5881 ggacacgagc gagaaccagt gcgcggtctc gtagtcgtcg aaccggacgc cgttcacgat
     5941 cggtgcgaac cagcgcggca gcgcgacgag cagcccggcg aaaagcccgg ccgccccgcc
     6001 gtagacgacg cccgcactca tcaatccgac gagtggccac gcgaacccga tggtgcgcac
     6061 gctcgagaac gcctcaccga ccgagttcgt gtgctcgtag acgatgccgc cgccgatgcc
     6121 gagcaacatc gcgagcgaca gctcgagacc gatcgcggcg ggacgctcga gccactccgg
     6181 gcgccgctcg acgagcacgg tcgccgccgc ggtccacgcg agcgtcatgc ccaagagcga
     6241 ccacgccagc accggacgtc cgagatcggc gcgattgagc aacatgacga ctgccatcca
     6301 gacccatgcg ccccagcgga agaccgccat cccgcgaagc atcccccgct gcacgaagaa
     6361 aagactaagc ccactagtct tcgcatatgg ccgaccaggc ccagttcgcc gagctcacca
     6421 tgccgttcat gtcggcgttg tatgcggccg cgctccggat gacgcgcaac ccgagtgacg
     6481 cagaggatct cgtgcaggag acgtacctgc gtgcgtaccg cgggttcgaa ggctttcgcg
     6541 agggcacgaa cctcaaggcg tggctctaca agatcctcac gaacacgttc atcaaccagt
     6601 accgggcgaa gaagcggcgt cccgacgaag tcgacctcga cgacaccgag gacttctact
     6661 tgttccggcg cctcggcggc ctcgagaacg tcgacgcgac ccgcaccgcg gaaaccgaag
     6721 tgctcgacgc gattcccgac gcggaagtga agg
//
LOCUS       DASUGA010000264         3341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1973702, whole genome shotgun sequence.
ACCESSION   DASUGA010000264 DASUGA010000000
VERSION     DASUGA010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1973702"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VFX21_05180"
     CDS             complement(<1..364)
                     /locus_tag="VFX21_05180"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX5095381.1"
                     /translation="MTTAPTTRRFGQSALLTPANLVTMSRIVVAIPTFALIRDRGSSW
                     LTVALWFAITSTDSLDGWLARRDGATRSGAFLDPIADKLIVLGGLAVLADRGDFPWWP
                     VAVIALRELGISLYRSIAG"
     gene            complement(361..1656)
                     /gene="rimO"
                     /locus_tag="VFX21_05185"
     CDS             complement(361..1656)
                     /gene="rimO"
                     /locus_tag="VFX21_05185"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX5095382.1"
                     /translation="MPDARPRFFVTTLGCPKNAVDSDKVVASLLADGLTEAENAEDAD
                     LVVVNTCAFIEAARQESIDVALALADVKRADAKLVLTGCMAERYGDELAAALPEADAV
                     VGFAGEGSLSQVVLKRKPTGVRDLLELPRPAPSAPWAYVKVAEGCDRACAFCAIPSFR
                     GTQRSRRPADIETEARDLVERGASELVLVAQDLAWYGRDAGEQGALVALLHRLDALAS
                     AGLSRIRLLYLYPSEVRDPLIATMLGLPTVVPYFDLSLQHASAPLLRRMKRWGDGDRF
                     RRLVDDIRARDGDAVFRSSFIVGFPGETERDHEQLLSFLGDVHLEWAGFFPFSPEAGT
                     AAVGLDGAVAPELIAERLRECAEVQEPLTAAARAALVGETVEVLVDGHEDGGLVGRTH
                     REAPEIDGIVRIMGAEGRPGALVKVRVTDVEGPDLEAVA"
     gene            1748..2209
                     /locus_tag="VFX21_05190"
     CDS             1748..2209
                     /locus_tag="VFX21_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095383.1"
                     /translation="MQDSPTVWCPSCGAEYRVGTTQCADCRVALAGTPPEHFGDTFEE
                     TGEESALVEMGEWPRIQAQILRRRLESANVPVMIEYSDTDIDATGVLIVPEEHAEFAA
                     AVINEIDVDDEVPDTSPYAYITRLEEHLSAAAELIGELRTRLDQLEAEGKL"
     gene            complement(2206..3135)
                     /locus_tag="VFX21_05195"
     CDS             complement(2206..3135)
                     /locus_tag="VFX21_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095384.1"
                     /translation="MILLAGSALTACSVRSSSDRSVAAASTTTVATAVGAPDPVPEVV
                     TRLTGNRLPSPLALADGDAWLGMHALEGDRPVIRLERRAFDSGALIGTVDVPQEGGFA
                     MASYKDVVYLVGGGDGAVPETTISALDARDESVVFTRALETPCSCAIAVNDAGVWLGA
                     NGGNYLVRADRETGKQVAEIELPRAAITISADEEFVRAALDDGTTALVDPATNAIAEI
                     SPVDPTGLTFTPRSTAVAGDGTWSVGAGNRIARTRDGDTTTYILQPDDTFDQGADELQ
                     FASSQYGFDQVVGDANRLWIAQQGAVLVVEIPA"
BASE COUNT          547 a         1172 c         1156 g          466 t
ORIGIN      
        1 ggccggcgat cgagcggtac aacgagatgc ccagctcgcg aagcgcgatg acggccaccg
       61 gccaccaggg gaagtcgccg cggtcggcca gcaccgcgag gccaccgagc acgatgagct
      121 tgtcggcgat cgggtcgagg aacgcgcccg aacgcgtcgc gccgtcgcgc cgcgccagcc
      181 agccgtcgag gctgtcggtc gacgtgatcg cgaaccacaa cgccaccgtg agccagctgg
      241 agccgcggtc gcggatcaag gcgaacgtcg ggatcgcgac gacgatgcgc gacatcgtga
      301 ccaggttcgc cggagtgagg agcgcgctct gcccgaagcg gcgcgtcgtc ggtgccgtcg
      361 tcacgcgacg gcctccaggt cgggaccctc gacgtcggtg acgcgcacct tcaccagcgc
      421 gccgggccgt ccttctgccc ccatgatgcg aacgataccg tcgatctcgg gcgcctcgcg
      481 atgcgtgcgg cccacgaggc cgccgtcctc gtgcccgtcg accaacactt cgacggtctc
      541 gcccaccagc gccgcacgcg ccgcggcggt gagtggctcc tgcacctccg cgcactcgcg
      601 caggcgctcg gcgatcagtt cgggcgcgac ggcgccgtcg agaccgaccg cggccgtgcc
      661 cgcttcgggc gagaacggga agaagccggc ccactcgaga tggacgtcgc cgaggaacga
      721 gagcagttgc tcgtggtcgc gctcggtctc gccggggaag ccgacgatga acgacgaacg
      781 gaacaccgcg tcgccatcac gggcgcggat gtcgtcgacg agacggcgga agcggtcgcc
      841 gtcgccccag cgcttcatgc ggcgcagcaa cggtgcggac gcgtgttgca acgacagatc
      901 gaagtagggc acgacggtcg gcaggccgag catcgttgcg atcagtgggt cacgcacctc
      961 cgacggatac aggtacagca gccggatccg cgacagcccc gcgctcgcca gcgcgtcgag
     1021 acggtgcaac agcgcgacca gcgcgccctg ctcaccggca tcacgtccgt accacgcgag
     1081 atcctgcgcg acgagcacga gctcgctcgc gccgcgctcg acgagatcgc gcgcttcggt
     1141 ctcgatgtcg gccgggcgac gggatcgctg ggtgccgcgg aacgacggga tcgcacagaa
     1201 cgcgcacgcc cggtcgcatc cctcggcaac cttgacgtag gcccacggcg cgctcggcgc
     1261 cggacgcggc agctccaaca ggtcgcgcac accggtcggc ttgcgcttca acacgacctg
     1321 cgagagcgac ccctcgcccg cgaatccgac caccgcgtcg gcctccggca acgcggcggc
     1381 gagctcgtcg ccatagcgct cggccatgca gccggtgagc accaacttcg cgtccgcgcg
     1441 cttcacatcg gcgagcgcga gcgcgacgtc gatcgattcc tggcgcgccg cctcgatgaa
     1501 cgcgcaggtg ttgacgacga cgaggtcggc gtcctcggcg ttctccgcct cggtgagccc
     1561 gtcggcaagc agtgacgcga cgaccttgtc ggagtcgacg gcgttcttcg gacaaccgag
     1621 tgtcgtcacg aagaagcgtg gtcgggcatc gggcacggtc gaacatcgta ccggcgaggg
     1681 ccttctacac tcctttcatg tgcggacgca gcggagccac ggacggcgta gcgagggagc
     1741 cgtagtcatg caggactccc cgaccgtgtg gtgcccgtcg tgcggcgccg aataccgcgt
     1801 cggcacgacg cagtgcgccg attgccgcgt ggcgctcgcc gggacgccgc cggagcattt
     1861 cggggacacg ttcgaagaga ccggcgagga gtcggcgctc gtcgagatgg gcgaatggcc
     1921 gcgcatccag gcgcagatcc tgcggcgccg gctcgagagc gcaaacgtgc cggtgatgat
     1981 cgaatactcc gacaccgaca tcgatgccac cggtgtgctc atcgtccccg aggagcacgc
     2041 ggagttcgcg gccgcggtca tcaacgagat cgacgtcgac gacgaagttc cggacacgtc
     2101 tccgtacgcg tacatcaccc gcctcgagga gcacctgtcg gccgcggccg agttgatcgg
     2161 cgagctgcgc acgcgcctcg accagctcga ggccgaaggc aagctctaag ccgggatctc
     2221 cacgacgagc accgcgccct gctgcgcgat ccacaaccgg ttggcgtcgc cgacgacctg
     2281 gtcgaagccg tactgcgagc tcgcgaactg gagctcgtcg gccccctggt cgaaggtgtc
     2341 gtccggctgg aggatgtacg tcgtcgtgtc gccgtcgcgc gtgcgggcga tgcgattgcc
     2401 cgcacccacc gaccatgtgc cgtcgccggc gacggccgtg ctgcgcggcg tgaacgtcag
     2461 cccggtcgga tcgaccgggc tgatctcggc gatcgcgttc gtcgcggggt cgacgagtgc
     2521 cgttgtgccg tcgtccagtg ccgcgcggac gaactcctcg tcggcgctga tcgtgatcgc
     2581 ggcgcggggc aactcgatct ccgcgacctg cttgcccgtc tcccgatccg ctcgcaccag
     2641 gtagttgccg ccgttcgcgc ccagccacac acccgcatcg ttgaccgcga tcgcgcacga
     2701 acacggcgtc tccagcgcgc gggtgaacac gacggactcg tcgcgggcgt cgagcgcgct
     2761 gatcgtcgtc tccggcacgg caccgtcacc gccgccgacg aggtagacga cgtccttgta
     2821 cgacgccatc gcgaagccgc cttcttgcgg gacgtcgacg gtgccgatga gggcgccgct
     2881 gtcgaacgcg cgccgttcga gccggatgac aggtcggtcg ccttcgaggg cgtgcatacc
     2941 aagccaggcg tcgccgtccg cgagcgccaa cggagagggc agacggtttc cggtcagccg
     3001 agtcacgact tccgggacgg gatcgggcgc cccgaccgcg gtcgcgaccg tcgtggtcga
     3061 cgcggcggcc accgaccggt cggaggacga tcgcaccgag cacgccgtga gggctgagcc
     3121 ggcgagaagg atcaccacaa ggactcgttg cacgcatgac aggacgccgc agtcctcgaa
     3181 tcggttgctt cgcgacatcg cgtgacgcga cgtaaccctc ttggcagacg cgcagtcgaa
     3241 ggagccggaa ggcgagtgtc cttccggaga accacctcgg aggtggacga tgtacgactt
     3301 cgcgatcgtc gcattgcttg cgctcgcgac tctcaagctc g
//
LOCUS       DASUGA010000265         5428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2044340, whole genome shotgun sequence.
ACCESSION   DASUGA010000265 DASUGA010000000
VERSION     DASUGA010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2044340"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VFX21_05200"
     CDS             complement(<1..801)
                     /locus_tag="VFX21_05200"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5095385.1"
                     /translation="MSDAVVLPSPCLVVLVGAAGAGKSTWAAEQFAPEQIVSSDALRA
                     VVGAGEDDVGASDDAFSILDQVVEHRCKRDLTTVVDTVGLDPTRRARWIAVARRHRIS
                     TACVIFTTSATECRARNRARKKTVPDKVLTGQARQVREQHDALYDEGFDFVLEPAIVR
                     IAPAHVAKTVELEARQAEAPVGMVFGLQIPAYTWPGGPHEMQWRLRSIARAAEDAGFA
                     SIWVMDHFRQIPSFGPAWDDMLESYTTLAFLAGVTEKVRLGTLVSGITH"
     gene            complement(826..1080)
                     /locus_tag="VFX21_05205"
     CDS             complement(826..1080)
                     /locus_tag="VFX21_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095386.1"
                     /translation="MRSQYLDRIREIPMFGHCSTRELTEIARLIDRIELPAGTALASN
                     TRELLLTTAPTEVLVIDRRALAAVLELVADDEIARHMSHV"
     gene            1217..1588
                     /locus_tag="VFX21_05210"
     CDS             1217..1588
                     /locus_tag="VFX21_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017569231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5095387.1"
                     /translation="MLDHLSIQCADVTASAAFYDAVFAPLGAGRIMDFGDVIGYGNDG
                     RPYFWIGPATTTGEAREAHIAFVAPDRDAVVAFRDAAVQAGAEVLHEPRVWPEYHPNY
                     FGAFVRDPDGNNVEAVCHTPA"
     gene            1674..3116
                     /locus_tag="VFX21_05215"
     CDS             1674..3116
                     /locus_tag="VFX21_05215"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5095388.1"
                     /translation="MATHDARLFIAGRYTEGTGDRAVPVVSPVTGEHLADVPVPSAAD
                     VDTAVAAARAAFDDYRHWSTYERAELCHRVAALIEANADELARLTTLEQGKPLEAEAR
                     GDVADSAELFAVSAEDAKRLYGSIIPSTARDKRIFTYRQPVGVWAGITPWNFPLMIMA
                     EFLAPALATGNAIVCKPPEHTPLACLRLGALLEEAGVPPGLVSIVPGAGATGEALVTH
                     AGVDAIGFVGSSATAERIVRAAGLKRTVIEASGNGPVIVCADADVEKAAEAAAYGAYW
                     NAGQVCCATERVIVDRDVHDDFVAALDDAAAAAVLGDPFAAATTLGPLNNEPTAAKMD
                     RHVADALDGGATLRRGGRRAEGFPTKLYYEFTVLDGVREDMLVAREESFGPVVPVLSA
                     NGDDDALRIANADPLGLQAAVFTSSLSRAFRFSESLRCGSVIVNDSTDYFENAMPFGG
                     AGGTRTGWGRVGGLFQLADMTDVRCTILSL"
     gene            3113..3988
                     /locus_tag="VFX21_05220"
     CDS             3113..3988
                     /locus_tag="VFX21_05220"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5095389.1"
                     /translation="MSALDGGRLFHVNVNCRSLARSRPFYEALGLTAGVQTAPEKPQP
                     GLAFGLRQAWWDAWIMTGARGFEGAAVDLLEWKEPVPAGAPPASFTETGWQRLGLAMP
                     DLDRVVAQVPALGGAVRSAPHSHTLDDGRTVKLAIIEDPDGTAVECIEGSASRVSFVA
                     VSCRNLDRSTACYAALGFREVARFTSHGEFVMDEVMLAAPGGGEAHVLLVHVVEPGPQ
                     VAPARKANTVGIWRAAFLVPDIDAAWAAVAAVDDAARLSPPVAMAMGEGLPELRFGCF
                     RGPDGEVLELIEQPG"
     gene            3985..>5428
                     /locus_tag="VFX21_05225"
     CDS             3985..>5428
                     /locus_tag="VFX21_05225"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEX5095390.1"
                     /translation="MTSRRDFLRHSAIGALGLTSVGALLNACGGDDGDGNEATGAGTT
                     TVPPTVPLDPDTPWWLQGNFAPVQHEVESVDLQVHGEIPAALSGLYVRNGSNPLTGWA
                     PHWFLGDGMVHGVMLDGGKASWYRNRYVQTALLAAGGGLTASGAPGGASSLSNVSMIH
                     HAGKLLALGEVGLPYEVSPRDCSTVGVYDFGGAINGNVTAHPKIDPATKQMHFFGYEF
                     SAPFLEYYVADANGTLITNEAVDVKASTMIHDFAITEKDVVFWEFPILFDFDLAIEMV
                     NTPKSTTMPFVWTPSYGSRIGVMPLGGPASAIRWVEIDNGYVFHGVNAFRDGDDVVVD
                     VCRMDEVFVGATLGPPPAMHRWRINTAGPSLTFADEVLSDRPADLPSIDRRHTGRDYR
                     HSWRVEVGTRTDTAELKGVVHYDTKTGREQRYDPGPTKSGDEWLFVANGDEEGDGYLL
                     TYVYDATTDSSELVVLDARDVESGPIGRVEL"
BASE COUNT          834 a         1862 c         1895 g          837 t
ORIGIN      
        1 gtgcgtgatg ccgctgacga gcgtgccgag ccgcaccttc tccgtcacgc ccgcgaggaa
       61 cgcgagcgtc gtgtagctct cgagcatgtc gtcccacgcc gggccgaacg acgggatctg
      121 gcggaagtgg tccatgaccc agatcgacgc gaacccggcg tcctcggcgg cccgggcgat
      181 cgagcgcagc cgccactgca tctcgtgcgg accaccgggc cacgtgtagg cgggaatctg
      241 cagaccgaag accatgccca ccggcgcctc ggcctggcgc gcttcgagct cgacggtctt
      301 ggcgacgtgt gcgggtgcga tgcggacgat cgcgggttcg agcacgaagt cgaagccttc
      361 gtcgtacagc gcatcgtgct gctcgcgcac ctggcgcgcc tgaccggtga gcaccttgtc
      421 gggcaccgtc ttcttccgcg ctcggttccg ggcccggcat tcggtcgccg atgtcgtgaa
      481 gatcacgcac gcggtcgaga tgcgatggcg ccgcgccacc gcgatccatc gcgcgcgccg
      541 cgtggggtcg agaccgacgg tgtcgacgac cgtcgtgaga tcacgcttgc agcgatgctc
      601 gacgacctgg tcgaggatcg agaacgcgtc gtcgctcgcg ccgacgtcgt cttcgccggc
      661 gccgacgacc gcgcgcaggg catcgctcga gacgatctgc tcgggtgcga actgctcggc
      721 cgcccacgtg ctcttcccgg caccggccgc gccgacgagc acgacgaggc acggtgacgg
      781 caacacgacg gcgtcggaca cgccatcatc ctgccgcgtg ccggctcaga cgtgagacat
      841 atggcgcgcg atctcgtcgt cggcgacgag ctcgagcacc gctgcgagcg cccgccggtc
      901 gataaccaat acttcggtcg gtgcggtcgt cagcagcagc tcacgggtgt tcgacgcgag
      961 tgcagtgccc gccgggagct cgatgcggtc gatcagccgg gcgatctccg tcagctcgcg
     1021 cgtcgagcag tgcccgaaca tcgggatctc ccggatccgg tcgagatact gcgagcgcac
     1081 gttgcgagtc ataggggagg tgaggaattg tggccgcaac ggcgtccgcg gtgcgaacga
     1141 ttttccatct cccacgacgg gtggagacga actcgcccgg ctgccattga gttcgcgaca
     1201 ggaaggcgat tgcatgctgc tcgaccatct ctccatccag tgcgccgacg tcacggccag
     1261 cgcggcgttc tacgacgcgg tgttcgcgcc gctcggcgcc gggcgcatca tggacttcgg
     1321 cgacgtgatc ggctacggga acgacggccg gccgtacttc tggatcggcc ccgcgacaac
     1381 cactggcgag gcgcgcgaag cgcacatcgc gttcgtcgcg cccgaccgcg acgcggtcgt
     1441 tgcgttccgg gacgcggccg tgcaggcggg cgccgaagtg ttgcacgaac cacgcgtgtg
     1501 gccggagtac cacccgaact atttcggcgc gttcgtacgc gaccccgacg gcaacaacgt
     1561 cgaggccgtc tgccacacac ccgcctagat cgaacagggg ccgatgtcgt cgtggtcggc
     1621 gacgacgcgc cccgccgtgc ccctcgaccg gttctccgcc taccgtctcg cccgtggcga
     1681 cccatgacgc gcggctgttc atcgccggcc ggtacaccga gggcaccggc gaccgcgccg
     1741 taccggtcgt ttcgccggtc accggcgagc atctcgccga cgtgccggtg ccgtcggccg
     1801 ccgacgtcga caccgcagtc gcggcggcgc gcgcggcatt cgacgactac cggcactggt
     1861 cgacgtacga gcgcgcggag ctgtgccacc gcgtcgcggc actgatcgaa gccaacgccg
     1921 acgagctcgc gcggctgacc acactcgaac agggcaagcc gctcgaagcc gaagcacgcg
     1981 gcgatgtcgc cgactcggcc gagctgttcg cggtgtcggc cgaggacgcc aagcgtctgt
     2041 acgggtcgat catcccgagc acggcacgcg acaagcgcat cttcacgtac cgccaacccg
     2101 tcggcgtgtg ggccggcatc acgccttgga acttcccgtt gatgatcatg gccgagttcc
     2161 tcgcgcccgc gctcgccacc gggaacgcga tcgtgtgcaa gccgccggag cacacgccgc
     2221 tcgcgtgcct gcgcctcggt gcgctgctcg aagaagccgg cgtgccgccc gggctggtgt
     2281 cgatcgtgcc gggtgccggc gcaaccggcg aagcgctcgt cacgcacgcg ggcgtcgacg
     2341 cgatcggttt cgtcggctcg tcggccaccg cggagcgcat cgtgcgcgcc gcgggcctga
     2401 agcgcaccgt gatcgaggcg tcggggaacg gtcccgtcat cgtgtgcgcg gacgccgacg
     2461 tcgagaaggc cgcggaggcc gccgcgtacg gcgcgtactg gaacgcgggc caggtgtgct
     2521 gcgcgaccga gcgcgtgatc gtcgaccgcg acgtgcacga cgacttcgtc gccgcgctgg
     2581 acgacgcagc cgcggccgcg gtgctcggcg acccgttcgc cgccgcgacg accttggggc
     2641 cgctcaacaa cgaaccgacg gccgcgaaga tggaccgtca cgtggcggac gcgctcgacg
     2701 ggggcgcgac gttgcgccgc ggcgggcggc gcgcggaggg gttcccgacg aagctgtact
     2761 acgagttcac ggtgctcgac ggtgtgcgcg aagacatgct cgtcgcgcgc gaggagtcgt
     2821 tcgggccggt cgtcccggtg ctgagcgcaa acggagacga cgacgcgttg cggatcgcga
     2881 acgcagatcc gctcggtctg caggcggcag tgttcacctc gtcgctgtcg cgcgcgttcc
     2941 ggttctcgga gtcgctccgt tgtggatcgg tgatcgtgaa cgactcgacc gactacttcg
     3001 agaacgcgat gccgttcggc ggtgcgggtg gcactcgcac gggatggggc cgagtcggcg
     3061 gtctgttcca gctcgccgac atgaccgacg tgcgctgcac cattctctcg ctatgagcgc
     3121 gctcgacggc ggccgcctgt tccacgtcaa cgtcaactgc cgttcgctcg cgcggtcgcg
     3181 gccgttctac gaagcactcg gtctgaccgc cggtgtgcag accgcgcccg agaagccgca
     3241 gccgggcctc gcgttcggat tgcgtcaggc atggtgggac gcgtggatca tgaccggtgc
     3301 gcgcggcttc gagggcgcgg ccgtcgactt gctcgagtgg aaggagccgg tacccgcggg
     3361 agcgccaccc gcgtcgttca cggaaacggg atggcagcga ctcgggctcg cgatgcccga
     3421 cctcgatcgc gtggtcgcgc aggtgcccgc gctcggtggt gcagtgcgca gtgcgccgca
     3481 ctcgcacacg ctcgacgacg gccgcacggt gaagctcgcg atcatcgagg atcccgacgg
     3541 cactgcggtc gagtgcatcg aaggcagcgc ctcgcgtgtt tccttcgtcg cggtgagctg
     3601 ccggaacctc gatcgatcga cggcctgcta cgccgcgctc ggcttccgcg aggtcgcacg
     3661 gttcacgtcg cacggcgaat tcgtgatgga cgaggtcatg ctcgcggcgc ccggtggtgg
     3721 agaggcacac gtcctgctcg tccacgtcgt ggagcccggg ccgcaggtcg cgccggcccg
     3781 caaggccaac acggtcggga tctggcgcgc cgcgttcctc gtccccgaca tcgacgcggc
     3841 gtgggcagcg gtcgcagccg tcgacgacgc cgcccgactg tcgccgccgg tcgcgatggc
     3901 aatgggcgag ggattgcccg agttgcgttt cggttgcttc cggggtcctg acggtgaggt
     3961 cctggaactg atagagcaac ccggatgacg tcccgccgcg acttcctccg tcacagcgcg
     4021 atcggcgcgc tcggccttac gagcgtgggc gcgttgctga acgcgtgcgg cggtgacgac
     4081 ggcgacggca acgaggccac cggcgcgggc accacgacgg tgccgccgac cgtgccgctc
     4141 gaccccgaca cgccgtggtg gttgcagggg aacttcgcgc cggtgcaaca cgaggtcgag
     4201 tcggtcgacc tgcaggtgca cggcgagatc cctgccgcgc tctccggact gtacgtgcgc
     4261 aacggatcga acccgctgac cggctgggcg ccacactggt tcctcggcga cggcatggtg
     4321 cacggcgtca tgctcgacgg cgggaaggcg tcgtggtacc gcaatcgcta cgtgcagacc
     4381 gcgttgttgg ccgcgggcgg cggcctcact gcatcgggcg caccgggtgg cgcatcgagc
     4441 ctcagcaacg tgtcgatgat ccatcacgcg ggcaagctgc tcgcgctcgg agaagtcggg
     4501 ctgccctacg aagtctcacc tcgcgactgc tcgaccgtcg gtgtctacga cttcggtgga
     4561 gcgatcaacg gcaacgtcac cgcgcacccg aagatcgatc ccgccacgaa acagatgcac
     4621 ttcttcggct acgagttctc ggcgccgttc ctcgagtact acgtcgccga cgcgaacggc
     4681 acgctgatca cgaacgaagc cgtcgacgtc aaggcgtcga cgatgatcca cgacttcgcg
     4741 atcaccgaga aggacgtcgt cttctgggag ttcccgatcc tgttcgactt cgacctcgcg
     4801 atcgagatgg tgaacacgcc gaagtcgacg acgatgccgt tcgtatggac gccatcgtac
     4861 gggtcacgca tcggcgtgat gcccctcggc ggaccggcct cggcgatccg ctgggtggag
     4921 atcgacaatg gctacgtgtt ccacggcgtg aacgcgttcc gcgacggtga cgatgtcgtc
     4981 gtcgacgtct gccggatgga cgaagtgttc gtcggtgcaa cgctgggtcc gccgccggcg
     5041 atgcatcggt ggcggatcaa caccgcgggg ccgtcgttga cgttcgccga cgaggtgctc
     5101 tccgaccgcc ccgccgacct gccgagcatc gaccggcggc acaccggccg cgactatcgc
     5161 cacagttggc gcgtcgaagt cggcacccga accgacaccg ccgagctcaa gggcgtcgtc
     5221 cactacgaca cgaagaccgg ccgcgagcag cgctacgacc cgggacccac gaagtcgggc
     5281 gacgagtggt tgttcgtcgc gaacggcgac gaagaaggcg acggctatct gctcacctac
     5341 gtctacgacg cgacgaccga cagctcggag ctggtcgtac tcgatgctcg agacgtcgag
     5401 agcggaccga tcgggcgcgt cgagctcc
//
LOCUS       DASUGA010000266         2753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_840556, whole genome shotgun sequence.
ACCESSION   DASUGA010000266 DASUGA010000000
VERSION     DASUGA010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840556"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /gene="manB"
                     /locus_tag="VFX21_05230"
     CDS             complement(<1..332)
                     /gene="manB"
                     /locus_tag="VFX21_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016430318.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX5095391.1"
                     /translation="MSLDSIFKAYDVRGVYPDEIDEQIARKIGNAFAHFTGAQTVVVG
                     RDMRPSSEPLVEAFIEGALLAGADVTDIGLCSTDLVYFASGTLDAPGAMFTASHNPAQ
                     YNGIKLCRS"
     gene            complement(418..>2753)
                     /locus_tag="VFX21_05235"
     CDS             complement(418..>2753)
                     /locus_tag="VFX21_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095392.1"
                     /translation="FTIGLAFALFALGALAKTLDTGRKPWLPAALMALAVMSHIVVGI
                     FMGLAAILLWLTRRPFRTWRLAVPVGFTAAALTAVWTLPLAATEHYTQSMRYTKLQVR
                     GSGWKLPSFLEFGPLGPVKHTIEGLVNGLEAPAIDPNTNPARHFSPTLWLPGWIWLLV
                     AIAVVAGAWYRRRSTGVLLLLGLVFGIMFVQWPEKAIWNTRFLPFWILSWAFVAAMGG
                     TELLRLGSKLVQWSYRWIRDGDLQDARARAWAEVATAPADDTTVSEETRKDAAWALAE
                     RRFDRGPDGWEPPARLNAENIARRGRMIGAGALAVLLALSGMFLVTRAWDARDNNPGI
                     AISGWAAWNYAGYERKGAYPQYKAIMDGVSEIANTPGEGPGRVLWEPSSGNPDAINSY
                     GTSLALELMPHFTDGKIGSMEGIYFESSATTSFHFLMVSNCAQHPSNPVRALVYGNPN
                     DDFDLCVKQMQDLGVRYYMVWTKEQRELADKTDDLTFVKEIPGEVQGTDLKGWRIYEV
                     ADSDLVEGMKTEPIVADVHPGKYSECWGQPWPDPNIREALLEQGWECDMAPAWMNRDL
                     IDVPFVEGPPPGLRAQGEKVMTGGPKDPPKEWQRIDGRELDQAEPRAIDDPAKVSNVK
                     RDVDSIEFDVDQIGKPVVVKESFFPNWKVEGAKGPYRMSPNVMVVVPTSQHVKLSYGL
                     TTADWLGRLITIFGLVGLVLLFVWKGAVRYGAGTEDAPVGDQPDENGGRDAADDSGDD
                     SPEGDSPDGDDGDDPDQSGDPPDGEPPDRWEPAPALP"
BASE COUNT          458 a          909 c          920 g          466 t
ORIGIN      
        1 gaccggcaga gcttgatgcc gttgtactgc gcggggttgt ggctggcggt gaacatcgcg
       61 ccgggcgcgt cgagcgtgcc cgacgcgaag tagacgaggt cggtggagca cagcccgata
      121 tcggtgacgt cggccccggc cagcagggca ccttcgatga aggcctcgac gagcggctcc
      181 gacgacgggc gcatgtcgcg gccgacaacg acggtctgtg cgcccgtgaa gtgggcgaag
      241 gcattgccga tcttgcgcgc gatctgctcg tcgatctcgt cgggatacac gccgcgcacg
      301 tcataggcct tgaagatcga atcgagcgac aacgctggct cctacagaag acgatcccgc
      361 gttccgcggt ccgggttcgg cgggtgtcgg ggagtcgcta cccgcgaacg gcggcggtca
      421 tggtagcgcc ggagccggtt cccaccggtc tggcggctca ccgtcgggcg gatccccgga
      481 ttgatcagga tcatcgccgt catctccgtc gggcgaatcc ccctcgggac tgtcgtcacc
      541 cgaatcgtcg gcggcgtcgc ggccaccgtt ttcgtcgggt tggtcaccca ccggtgcgtc
      601 ttcggtgccg gctccgtaac gaaccgcacc cttccagacg aacaacaaca cgaggccaac
      661 gaggccgaag atcgtgatga gtcgtccgag ccagtcggcg gtcgtgagtc cgtaactcag
      721 cttcacgtgc tgcgacgtcg gcacgacgac catcacgttc ggcgacatgc ggtacggacc
      781 cttcgcgccc tcaactttcc agttcgggaa gaaggattcc ttcaccacga cgggcttgcc
      841 gatttggtcg acgtcgaact cgatcgagtc gacgtcgcgc ttcacgttgc tcaccttcgc
      901 gggatcgtcg atcgcgcgcg gttcggcttg gtccagctcg cgaccgtcga tgcgctgcca
      961 ttccttcggc gggtccttcg gaccgccggt catgaccttc tcgccctgcg cgcgcaaccc
     1021 cggtggcggc ccttcgacga agggaacatc gatgaggtcg cggttcatcc acgccggcgc
     1081 catgtcgcat tcccagccct gctcgagcag cgcttcgcgg atgttcggat cgggccaggg
     1141 ctgaccccag cattcggagt acttgccggg atgcacgtcg gcgacgatcg gctcggtctt
     1201 catgccctcg acgagatcgc tgtcggcgac ttcgtagatg cgccaaccct tgaggtcggt
     1261 gccctgaacc tcgcccggga tctccttgac gaaggtcaag tcgtcggtct tgtcggcgag
     1321 ctcgcgttgc tccttggtcc acaccatgta gtagcgaacg ccgaggtcct gcatctgctt
     1381 gacgcagaga tcgaagtcgt cgttggggtt gccgtacacg agcgcgcgca ccgggttcga
     1441 cgggtgttgc gcgcaattgc tgaccatcaa gaagtggaac gacgtcgtgg ccgacgactc
     1501 gaagtagatg ccttccatcg agccgatctt gccgtcggtg aagtgcggca tcagctcgag
     1561 cgcgagtgac gtcccgtaag agttgatcgc gtcggggttg ccggacgacg gctcccacaa
     1621 cacgcggccc gggccttccc cgggcgtgtt cgcgatctcg gagacgccgt ccatgatcgc
     1681 cttgtattgc gggtacgcgc ccttgcgttc gtagccggcg tagttccacg cggcccagcc
     1741 cgaaattgcg ataccggggt tgttgtcgcg cgcgtcccac gcgcgcgtga cgaggaacat
     1801 gccgctcaac gcgagcagca ccgcgagcgc gccggcgccg atcatgcgac cgcgccgcgc
     1861 gatgttctcg gcgttcaggc gcgccggcgg ttcccagccg tcgggaccgc ggtcgaaacg
     1921 ccgctcggcg agcgcccagg ccgcgtcctt gcgcgtctcc tcgctgacgg tcgtgtcgtc
     1981 ggccggcgcg gtcgcgactt cggcccacgc gcgagcgcgc gcgtcttgca ggtcaccgtc
     2041 gcgaatccat cgatacgacc actgcacgag cttcgaaccg agacgcagca actccgtgcc
     2101 gcccatcgcg gcgacgaacg cccacgacaa gatccagaag ggcaggaacc gcgtgttcca
     2161 gatggctttt tccggccact ggacgaacat gatgccgaac acgagtccga gcagcaggag
     2221 cacaccggtc gaacggcggc gataccacgc gccggcgacg acggcgatcg cgacgagcaa
     2281 ccagatccag cccggcagcc acagcgtcgg cgagaagtga cgggccggat tcgtgttggg
     2341 gtcgatcgcg ggcgcctcca acccgttcac gaggccttcg atggtgtgct tcaccgggcc
     2401 gagcggaccg aactcgagga acgacggcag cttccatccg ctgccgcgta cctggagctt
     2461 cgtgtaccgc atgctctgcg tgtagtgctc ggttgcggcg agcggcagcg tccacaccgc
     2521 cgtgagcgca gcggcagtga aacccacggg aacggcgagc cgccaggtgc ggaacggacg
     2581 gcgggtgagc cagagcagga tggccgccag gcccatgaag atgccgacga cgatgtgcga
     2641 catgacggcg agcgccatca gtgccgcggg caaccacggt tttcgtccgg tgtcgagcgt
     2701 cttggccagc gcgccgagtg cgaacagcgc aaacgcgaga ccgatcgtga acg
//
LOCUS       DASUGA010000267         3258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_779421, whole genome shotgun sequence.
ACCESSION   DASUGA010000267 DASUGA010000000
VERSION     DASUGA010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779421"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VFX21_05240"
     CDS             complement(<1..872)
                     /locus_tag="VFX21_05240"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5095393.1"
                     /translation="MNLSFTPGEEAFAAEVRAWLAARVEVPPRFETIADEVDFGRRWQ
                     AELAAGRWVGIHWPEAYGGRGASPVQVAIFNMEYARSRALQPINRVGINLAGPTLLAH
                     GTEEQKQRWLPSILTADEIWCQLFSEPDAGSDLASLKTRAQRVDDGWLLSGQKVWTSY
                     AQFARWGICLARTDADAPKHKGISYLVVDMQAPGIEIRPLVQITGDAEFNEVFFDDVF
                     VPDGHLVGALHNGWAVANTTLAHERGTSFPFKEQVVHEVYLDELWQLAAQRGSLDDVE
                     IADALAQSFVELRV"
     gene            complement(869..2542)
                     /locus_tag="VFX21_05245"
     CDS             complement(869..2542)
                     /locus_tag="VFX21_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5095394.1"
                     /translation="MLRRNAEEHPDNVAIKFGDRQWTHREYFEESCRFATLFDERLPK
                     SGPRHVAVLLDNTPDYLFAFGGAALLGAAVVGVNHTRRGEHLRRDVAHTHCSLVITEP
                     RHEDLLAPIADALPPVLTSTRFADRDDPAPAIGASLDDAIASVSHDDLGLDPDIDAIW
                     ALIFTSGTSDAPKAVICSQRRLLVTGNRMSMIMDLGPDDVGYVCMPLFHSNAVQVGWA
                     PSIVKACAVGLGRKFSASKFLPDVRRYGSTYFNYTGKPLAYLLAQPERPDDADNSLRV
                     AFGNEGSPEVVESFAQRFGVDVIDAYGATEGGVAVNRDAEPRPGALGLAPEAVKIVDE
                     EGNEKARAQLDERGRVQNADECVGEIVNTAGAGPFEGYYNNSDATEKTTRHGWYWSGD
                     LGYKDADGYLYFAGRNADWIRVDGENFPAGPIEEALRKAPDVILSAVYGVPDDQAGDQ
                     AMAGLVLAAGATFDGVAFAQWLDEQEAIGPKWRPRYVRILRDPPTTGTNKIVKRTLAQ
                     QKFRHDLVGGDELYVRPRGADSYTPFSESDERALHESFVAYQRERFWEL"
     gene            complement(2711..2905)
                     /locus_tag="VFX21_05250"
     CDS             complement(2711..2905)
                     /locus_tag="VFX21_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095395.1"
                     /translation="MGEIPTAAHDIHVDVIVTPERVIRCPRGRGHRLPQLQWAELTSE
                     KIAAIPLLKLLARDANNAVP"
BASE COUNT          504 a         1115 c         1103 g          536 t
ORIGIN      
        1 aagacgcgca gttcgacgaa cgattgcgcg agcgcgtccg cgatctcgac gtcgtcgagg
       61 ctcccgcgct gtgccgcgag ctgccagagc tcgtcgagat acacctcgtg cacgacttgt
      121 tccttgaacg ggaacgacgt accccgttca tgggccagtg tcgtgttcgc gactgcccag
      181 ccgttgtgca gcgcgcccac gagatggccg tcgggcacga acacgtcgtc gaagaacacc
      241 tcgttgaact cggcgtcacc ggtgatctgc accagtgggc ggatctcgat gccgggcgcc
      301 tgcatgtcga cgaccaggta ggagatgccc ttgtgcttcg gcgcgtcggc gtcggtgcgg
      361 gcgagacaga tgccccagcg cgcgaactgc gcgtaggacg tccacacctt ctgccccgac
      421 agcagccagc cgtcatcgac gcgctgcgca cgcgtcttca aggaggcgag gtcggagccc
      481 gcgtcgggct cgctgaacag ctggcaccag atctcgtccg cggtgaggat cgacggcagc
      541 caccgctgct tctgctcctc cgtcccgtgc gcgagcagtg tcgggccggc gaggttgata
      601 ccgacgcggt tgatcggttg cagcgcgcgc gaccgcgcgt actccatgtt gaagatcgcg
      661 acctgcaccg gcgatgcgcc gcgaccgccg tacgcctccg gccagtggat gccgacccac
      721 cgcccggccg cgagctcggc ctgccagcgc cgaccgaagt cgacctcgtc cgcgatcgtc
      781 tcgaaccgcg gcgggacctc gacgcgcgcg gccagccatg cgcggacctc ggccgcgaac
      841 gcttcttccc cgggcgtgaa cgagaggttc acagttccca gaagcgttcg cgctggtacg
      901 caacgaacga ctcgtgcagc gcgcgctcgt cgctctcgga gaacggcgtg tagctgtcgg
      961 caccccgcgg ccgcacgtag agctcgtcac cgccgacgag atcgtgacgg aacttctgct
     1021 gcgccagcgt gcgcttcacg atcttgttcg tgccggtcgt cggcggatcg cgcaggatgc
     1081 gcacatagcg cggccgccac ttggggccga tcgcctcctg ctcgtcgagc cactgcgcga
     1141 acgcgacacc gtcgaacgtc gcgccggcgg cgaggacgag gccggccatc gcctggtcgc
     1201 cggcttggtc gtccggcacg ccgtagacgg ccgagagaat gacgtcgggt gctttgcgta
     1261 acgcttcctc gatcggtccc gcgggaaagt tctcgccgtc gacgcggatc cagtccgcgt
     1321 tgcgccccgc gaagtaaagg tagccgtcgg cgtccttgta tccgaggtcg cccgaccagt
     1381 accagccgtg acgcgtcgtc ttctccgtcg cgtcgctgtt gttgtagtag ccctcgaacg
     1441 gacccgcacc cgcggtgttg acgatctcgc cgacacattc gtcggcgttc tgcacgcggc
     1501 cgcgctcgtc gagttgggcg cgcgccttct cgttgccttc ttcgtcgacg atcttcaccg
     1561 cctcgggtgc gagaccgagc gcgccggggc gcggctcggc gtcgcggttg acggccacgc
     1621 cgccttcggt cgcgccgtag gcgtcgatca cgtcgacccc gaagcgctgg gcgaacgact
     1681 cgacgacctc cggcgaaccc tcgttgccga acgcgacgcg caacgagttg tccgcgtcgt
     1741 cgggccgctc gggttgggcg agcagatagg ccaatggctt gccggtgtag ttgaagtagg
     1801 tcgagccgta gcggcgcacg tcgggcaaga acttcgatgc cgagaatttc cggccgagcc
     1861 cgaccgcgca ggccttgacg atcgacggcg cccacccgac ctgcaccgcg ttggagtgga
     1921 acaacggcat gcagacgtag ccgacgtcat cgggcccgag gtccatgatc atcgacatgc
     1981 ggttacccgt gacgagcagg cgacgttgcg agcagatcac cgctttgggc gcgtcggagg
     2041 tgcccgacgt gaagatcagc gcccagatcg cgtcgatgtc gggatcgagc cccagatcgt
     2101 cgtgggacac cgacgcgatt gcgtcgtcga gcgacgcgcc gatcgccggc gcgggatcgt
     2161 cgcggtccgc aaatcgcgtc gacgtgagga ccggcggcag cgcgtccgcg atcggcgcca
     2221 gcaagtcttc gtggcgcggc tcggtgatca ccagcgaaca gtgcgtgtgc gcgacgtcgc
     2281 gccggaggtg ctcgccccgg cgcgtgtggt tcaccccgac gaccgccgcg cccaacagcg
     2341 cggcgccgcc gaacgcgaac aagtaatcgg gggtgttgtc gagcagcacc gcgacgtggc
     2401 gcggtccgga tttggggagc cgctcgtcga acagggtcgc gaaccggcac gactcctcga
     2461 agtactcgcg gtgggtccac tgccggtcgc cgaacttgat cgcgacattg tccgggtgct
     2521 cctcggcgtt gcgccgcagc aatgcggccg cgttgggtgc aaccagcggc gcctgaccag
     2581 gagccgggac cgatcgggat cgcaaacctg acatcaacgt cagacaataa ctctctgtcg
     2641 ccttcgacct gctcgcgtga gggagcccgg attgtcggcc ggcctcaggc tccgccagtg
     2701 cctccgcaag ctacggcacg gcgttgttgg cgtctcgcgc caggagtttc agaagcggga
     2761 tcgccgcgat cttctcgctg gtcagctccg cccactgcag ctgcggcaag cgatggccgc
     2821 ggccgcgtgg gcagcggatg actcgttcgg gcgtcacgat cacgtcgacg tgtatgtcgt
     2881 gtgccgccgt cgggatctca cccacccgcg agtgcgcctc cgcggagaag cgttcatcat
     2941 gccaaatcgc gcggtcgcat ccagttctcg acgttgtgga cacgacggtt ccaggtctca
     3001 cggcgcggtg ccggagcttg cggaggcact cgcggagtcc tgaggcgggc caacaatccg
     3061 acctgcctca cgcgagcacg tcgaaggcga caaagaggaa tgcccggttc cgacatcgcg
     3121 gcccacacct gctcacgcag gtcggccttg cgcgccagca gctcggcgtc gccgcccgca
     3181 tcgtggtcgt gtcgcgccgg catgcggttg agcgtaaaac gtccacgtcc cgtcttgacg
     3241 tgcggtgccg gagcttgc
//
LOCUS       DASUGA010000268         2114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1960357, whole genome shotgun sequence.
ACCESSION   DASUGA010000268 DASUGA010000000
VERSION     DASUGA010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1960357"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VFX21_05255"
     CDS             <1..551
                     /locus_tag="VFX21_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEX5095396.1"
                     /translation="DPRSLKDRWRIGYVPQRLRLQPDLPATVEEVVRTGRLPRRGWWR
                     PPRHADREAVDHALESVALADLRHRRVGELSGGQQQRVLIAKAFAAEPDVLVLDEPIA
                     GVDAESQRLFRDSLVHLVAEHDATVLLVSHELGAVADDLDRVVVLKRTVVFDGAPNDL
                     AATGVHLGVHDADLPRWLEDLS"
     gene            548..1408
                     /locus_tag="VFX21_05260"
     CDS             548..1408
                     /locus_tag="VFX21_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX5095397.1"
                     /translation="MNLALTLTLPWPFDRDYMQLALIAGLVVGACGPLIGAFLVEKRM
                     ALLGDGLGHLAFAGVAIGLLTDVWPIWTALIAAVAGAIVVEWLRSHGRASGDLALAMI
                     FYGGIALAVVVASRTPEGSSVNIVPYLFGSILTVTASDVRVIVVLGVVIVATLLLAGR
                     ALFAIVLDEESARVAGLPVDALNTLLAALTAVTIVAAMRVVGVLLVAALMVLPVAASR
                     LVARSFRGTLVGAVLVGIASVVGGLAAARQWDLAPGGSIVLTTVAIFAVTAVIGSVRG
                     RSGALLRIDH"
     gene            1420..>2114
                     /locus_tag="VFX21_05265"
     CDS             1420..>2114
                     /locus_tag="VFX21_05265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5095398.1"
                     /translation="MRMKGERALVTGSTAGIGRAIALAFAREGAAVVVTGRNEERGAT
                     VVAEITASGGTATFDAADVHHEGECTALVERAATALGGLSVLVNNAAGGDVADARVAD
                     TDTAAWEAILRVDLTAPMWLARAAIPHLRAAGHGTIVNISSRQGERPSAGLAAYVAAK
                     AGLNGLTRAIAVEEAVHGIRCNTISPGYVLNDRRDADITPERRARFEGMHLTGLGTAE
                     DVAAAAVYLACAES"
BASE COUNT          297 a          730 c          722 g          365 t
ORIGIN      
        1 aagacccgcg ttcgctcaag gaccgctggc gcatcggtta cgtgccccag cggctgcggc
       61 tccagcctga tctgccggcc accgtcgaag aggtcgtccg taccggacgc ctgcctcgcc
      121 ggggttggtg gcgaccgccg cgccacgccg accgtgaggc cgtcgaccac gcgctcgagt
      181 cggtggcgct cgccgatctg cgccaccggc gcgtcggcga actgtccggc ggccagcaac
      241 agcgcgtgtt gatcgccaag gcgtttgcgg ccgaacccga tgtgctcgtc ctcgacgaac
      301 cgatcgccgg cgtcgacgcc gaatcgcaac ggctgttccg cgactcgctc gtgcacctcg
      361 tcgcggaaca cgacgcgacg gtgctcctcg tttcccacga actcggggcc gtcgccgacg
      421 atctggatcg cgttgtcgtc ctgaagcgca cggtcgtctt cgacggcgca ccgaacgatc
      481 ttgccgcgac cggtgtacat cttggcgtgc acgacgccga tctcccgcgc tggctcgagg
      541 acttgtcgtg aatctcgcgc tgacactgac actcccctgg ccgttcgacc gcgactacat
      601 gcagctcgcg ttgatcgccg gccttgtcgt cggtgcgtgc ggaccactca tcggcgcgtt
      661 ccttgtcgag aagcgcatgg cgctactcgg ggacggactc ggacacctcg ccttcgcagg
      721 ggttgccatc ggcttgctca ccgacgtgtg gccgatctgg accgcgctca tcgccgccgt
      781 cgccggcgcc atcgtggtcg agtggctgcg gtcgcacggc cgcgcgtcag gcgacttggc
      841 gctcgccatg atcttctacg gcggcatcgc gctggcagtc gtcgtcgcga gccgcacgcc
      901 ggaaggatcg agcgtgaaca tcgtcccgta tctgttcggc tcgattctga ccgtcaccgc
      961 gtccgacgtg cgcgttatcg tcgtgctcgg cgtcgtcatc gtcgcaacgc tgctgctcgc
     1021 gggacgcgca ctgttcgcga ttgtgctcga cgaagagtcg gcacgcgttg caggcctgcc
     1081 ggtggacgcg ctgaacacgt tgctcgcggc gttgaccgcc gtcacgatcg tcgccgcgat
     1141 gcgcgtcgtc ggggtgttgc tcgttgcggc actgatggtg cttccggttg ccgcgagccg
     1201 attggtggcc cgatcgtttc gagggacctt ggtcggcgcg gtactcgtcg gaatcgcttc
     1261 ggtcgtcggt ggtctcgccg ccgcgcggca atgggacctt gcgcccggtg ggtcgatcgt
     1321 gctgacaacc gtcgcgatct tcgcggtcac tgccgtgatc ggcagcgtgc gcggccggag
     1381 cggtgcactg ttgcgtatcg accactaacg tcgctcgcga tgcgcatgaa gggcgagcgg
     1441 gcgctcgtta ccgggtcgac cgccgggatc ggtcgggcaa tcgcgcttgc gttcgcacgc
     1501 gaaggcgcgg ccgtcgtcgt gaccggccgc aacgaagaac gcggtgcaac ggtggtcgcg
     1561 gaaatcaccg cgagcggggg cactgcgacg ttcgacgcgg cggacgtgca tcacgaaggc
     1621 gaatgcacgg cgctcgtcga acgggccgca accgcgttgg gcgggctcag tgtgctggtc
     1681 aacaacgcgg ccggcggtga cgtggccgac gcgcgcgtcg ccgataccga cactgcggcc
     1741 tgggaagcca ttctgcgggt cgatctcacc gcaccgatgt ggctcgcccg ggcggccatc
     1801 ccgcacctgc gagccgccgg ccacggcacc atcgtcaaca tttcgtcccg ccagggcgaa
     1861 cggcccagcg ccggattggc ggcgtacgtc gccgccaagg ccgggctcaa cggactcacg
     1921 cgcgcgatcg ccgtcgaaga ggcagtacac ggcattcgct gcaacaccat cagccccggc
     1981 tatgtgttga acgaccggcg cgacgccgac atcacacctg agcggcgggc ccgattcgaa
     2041 ggtatgcatc tcaccggact cgggacggcc gaagatgtcg ccgccgcggc cgtctacctc
     2101 gcgtgcgccg agtc
//
LOCUS       DASUGA010000269         3590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1677322, whole genome shotgun sequence.
ACCESSION   DASUGA010000269 DASUGA010000000
VERSION     DASUGA010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677322"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VFX21_05270"
     CDS             <1..580
                     /locus_tag="VFX21_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX5095399.1"
                     /translation="PIFGGMTLVDAKFTGAGPHLIAVRPKSFAAESAGGGAAAISNVG
                     AADAGRAAEAKVLEKHVEEQQGPKLEDATVVVSGGRGIGAAENYAPLVEELAKQLKGA
                     WGASRAIVDAGWVPYALQVGQTGKTVKPKVYIALGISGATQHLVGMKNSDNIIAVNKD
                     GEAPIFSVADLGIVGDVHKVVPALIEALKAKS"
     gene            865..1599
                     /locus_tag="VFX21_05275"
     CDS             865..1599
                     /locus_tag="VFX21_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095400.1"
                     /translation="MLSRHALRTNEAHLWWLRVREEPTAAIESVLDDGDRRALAGAAD
                     TEWRTLVVWRRALLRLVAGALVDVDPATVPLGRRCPTCGATDHGAPTIADCDVSLSAA
                     AADGIVVVAACAGLRVGVDVLARADGEAALFGARIVCTSADVDALDAIPDEHRGDAAL
                     HAVTQLDAYAKLGGLHHHAYPEISVSIDVFRSAVRAGDAAFWARRVDPGPGYVANLVA
                     DGRPRAVRGHDATDLLIDVMHGSSAA"
     gene            complement(1574..2305)
                     /locus_tag="VFX21_05280"
     CDS             complement(1574..2305)
                     /locus_tag="VFX21_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095401.1"
                     /translation="MSDPLVIAVDWSGANAAAAQRRAIWTTTIRDGRVVESVGGRTRA
                     DVVDALVQRDEPMIVGLDFSFSVPHWFACAHACHDVDAVWKLAQVRGDDWLRPTPPFW
                     RERCAVPLGQRFRRCETRLRGEGFQPKSLFQLVGNGQVGAGSVRGMPLLATLRGAGFA
                     VWPFDAAAERTVMEMYPSLLRARAPHLDTGSHPNEHARDATVAALVMWEHRDALARLD
                     ATKDPVVALEGDVWDPAATRPRSRA"
     gene            2329..3090
                     /locus_tag="VFX21_05285"
     CDS             2329..3090
                     /locus_tag="VFX21_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5095402.1"
                     /translation="MPDVILEARALVKDYRRARAVDGVDLVMHAGERVALLGPNGAGK
                     TTTLLMILGVISPDEGSVSIAGFPLARKRSKAAEHVGFAAGYLPLAERMRVSEYLRLY
                     GQIYGIADPTPQIDEGLARFRIEHLADAMGTELSSGQRTLIGIVRATLHKPRLLVLDE
                     PTASLDPDVALRVRNGLIELADANGCALLMTSHDMSDVEQVSERVIFLSHGRIIADGT
                     PQQIAAQYGHEDLEGVFLHLAERHETSHAQQSDHP"
     gene            3155..>3590
                     /locus_tag="VFX21_05290"
     CDS             3155..>3590
                     /locus_tag="VFX21_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095403.1"
                     /translation="MSNVTMIRLRAISRRHAYVLVRSPHRLFDVTLWPLVDVVLFGSL
                     AAFVGDGDATAANQTAGYLLAGIVCWHVIYQSQIALSTGFLEETWSRNLLNLMVTPLR
                     EVEYVAGVALFGMVKLFLGLAVMVLGALAFFSFHTWELGWGLI"
BASE COUNT          543 a         1229 c         1275 g          543 t
ORIGIN      
        1 gccgatcttc ggcggcatga cgctcgtcga cgccaagttc accggcgccg gtccgcacct
       61 catcgcggtg cgtccgaagt cgttcgcggc ggagtcggcg ggtggcggcg cggcggcgat
      121 ctcgaacgtc ggcgcggccg acgcgggacg cgcggccgag gcgaaggtgc tcgagaagca
      181 cgtcgaggag cagcagggcc cgaagctcga ggacgcgacg gttgtcgtct cgggcggtcg
      241 cggcatcggc gcggcggaga actacgcgcc gctcgtcgag gagctggcca agcagctcaa
      301 gggtgcatgg ggcgcgtcac gcgcgatcgt cgacgcgggc tgggtgccgt acgcgttgca
      361 ggtcggccag accggcaaga ccgtgaagcc gaaggtctac atcgcgctcg gcatctccgg
      421 cgcgacgcag cacctcgtcg gcatgaagaa cagcgacaac atcatcgccg tcaacaagga
      481 cggtgaggcg ccgatcttct cggtcgccga cctcggcatc gtcggcgacg tccacaaagt
      541 cgtccccgcg ctgatcgaag ccctcaaggc gaagagctaa ttacttgagc agatcgagcg
      601 ggagcgcggg actttcgtag tgggcgtccg actgggtgat caccagtcgg acgtttgctt
      661 cgtcggcgac cgcgcgacgg cggtcgaact cttcgttggc gtcgatcggc gccgggacag
      721 cgaatcgggc cggggtgcca agggcgcggg ctcggcgtcc ggaactcggt ccgccgcgcg
      781 gcagagaccg gctccacctg cgggtgggtg caatcacgac ccgcggcaga agcggtggga
      841 tcgagaaccg gtcatcttga tgacgtgctt tcccgccacg ccctgcgcac caacgaggct
      901 cacctgtggt ggctgcgggt ccgggaggag cccacggcgg cgatcgagtc ggtgctcgac
      961 gacggcgacc ggcgggcact ggccggcgcg gccgacaccg agtggcggac gctcgtggtg
     1021 tggcgccggg cgctgctgcg tttggttgcg ggcgcgctcg tcgacgtcga tcccgcgacc
     1081 gtgccgctcg ggcgtcgctg cccgacgtgc ggcgccaccg accacggcgc gccgacgatc
     1141 gcggattgtg acgtgtcgct gagcgccgcc gcggccgacg gcatcgtcgt ggtcgccgcc
     1201 tgcgccggcc tccgcgtcgg cgtcgacgta ctggcacgcg cggatggcga ggccgcgttg
     1261 ttcggcgcgc gcatcgtctg tacgtccgcc gacgtcgacg cgctggacgc catccctgac
     1321 gagcatcggg gcgacgccgc gctgcatgcg gtgacgcaac tcgacgcata cgccaagctc
     1381 ggcggcttgc atcaccacgc ttaccccgag atctcggtct cgatcgacgt gttccgttcg
     1441 gcggtgcgcg ccggcgacgc cgcgttctgg gcccgccgcg tggatccggg cccggggtac
     1501 gtcgccaacc tcgtcgccga cggccgcccg cgtgcggtgc gcggtcacga cgcgaccgac
     1561 ttgctcatcg acgtcatgca cggctcctcg gccgcgtagc ggccgggtcc cagacgtcac
     1621 cttcgagtgc gacgaccgga tccttcgtcg cgtccagacg cgccaacgcg tcacggtgct
     1681 cccacatcac gagcgcggcc acggtcgcgt cgcgcgcgtg ctcgttcggg tgcgacccgg
     1741 tgtcgaggtg aggtgcgcga gcgcgcagca gtgacggata catctccatg acggtgcgtt
     1801 cggcggcggc atcgaacggc cacactgcga acccggcgcc tcgcaacgtc gccagcagcg
     1861 gcatgccgcg cacggagccc gcgccgacct gcccgttgcc gacgagttgg aagagcgact
     1921 tcggttggaa gccctcgccg cggagtcgcg tctcgcagcg ccggaaccgt tggccgaggg
     1981 gaacggcgca ccgctcgcgc cagaacggag gcgtcggccg taaccagtcg tcgccgcgaa
     2041 cctgcgcgag cttccacacc gcgtcgacgt cgtgacaggc gtgcgcgcac gcgaaccaat
     2101 ggggtaccga gaacgagaag tcgaggccga cgatcatcgg ttcgtcgcgt tgcacgagtg
     2161 catcgacgac atcggcccgc gtgcggccac ctacgctctc gacgacgcgt ccgtcgcgaa
     2221 tcgtcgtcgt ccagatcgcc cggcgctgcg cggcggcggc gttggcgccc gaccagtcga
     2281 cggcgataac caacgggtcg gacacgcgcg gcacggtacg gtgccggcgt gcccgacgtc
     2341 atcctcgaag cccgcgccct ggtcaaggac taccgccgcg cgcgtgcagt cgacggcgtc
     2401 gatctcgtca tgcacgcggg cgaacgcgtc gcgctgctgg gcccgaacgg cgcgggcaag
     2461 acgacgacgc tgctgatgat cctcggggtc atcagtcccg acgaaggcag cgtgtcgatc
     2521 gccggattcc cgctggcgcg caagcgcagc aaggcggccg agcacgttgg cttcgcagcc
     2581 gggtatctcc cgctcgccga gcgcatgcgc gtcagcgagt acttgcgcct gtacggccag
     2641 atctacggga tcgccgatcc gacgccgcag atcgacgaag ggctcgcccg tttccgcatc
     2701 gagcatctcg cggacgcgat gggtaccgag ctgtcgtccg ggcagcgcac gctcatcggc
     2761 atcgtgcgcg cgacgttgca caagccgcga ttgctcgtgc tcgacgagcc gaccgcatca
     2821 ctcgatcccg acgtcgcgct tcgagtgcgc aacgggctca tcgagctggc cgacgcgaat
     2881 ggctgcgcgc tgttgatgac gagtcacgac atgagcgacg tcgaacaggt gagcgagcgc
     2941 gtcatcttcc tgtcgcacgg tcgcatcatc gccgacggca ccccgcaaca gatcgccgcg
     3001 cagtacggac acgaagacct cgaaggggtg ttcctccacc tggccgagcg gcacgagacc
     3061 agccacgcgc agcaaagcga ccacccatga acgtgcggac gcagcggagg ccttcttccg
     3121 gcgaagccgt gctcggctcg gagcggaggg agccatgagc aacgtcacga tgatccggct
     3181 gcgggccatc tcgcggcggc acgcgtacgt cctcgtgcgc agcccgcacc ggttgttcga
     3241 cgtcacgttg tggccgctcg tcgacgtcgt gctgttcggt tcgctcgccg cgttcgtcgg
     3301 cgacggcgac gcgactgcgg ccaaccagac cgcggggtat ctgttggccg gcatcgtgtg
     3361 ctggcacgtg atctaccagt cgcagatcgc gctcagcacg ggcttcctcg aagagacgtg
     3421 gagccgcaac ctcctgaacc tcatggtaac gccgttgcgc gaggtcgaat acgtcgcggg
     3481 cgtcgcgctg ttcgggatgg tgaagctgtt cctcgggctc gcggtgatgg tgctcggcgc
     3541 gctcgcgttc ttctcgttcc acacctggga gctcgggtgg gggctcatcc
//
LOCUS       DASUGA010000270         2408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1784831, whole genome shotgun sequence.
ACCESSION   DASUGA010000270 DASUGA010000000
VERSION     DASUGA010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1784831"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VFX21_05295"
     CDS             complement(<1..610)
                     /locus_tag="VFX21_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095404.1"
                     /translation="MAQRAQRLIDAAKNSPLPEGTYAVGAGLLVAGLAAYGFQILTFR
                     QLDESQYAALNGLWILIFIVTPGLFQPLEQEVARSLAHRNSQGRGGAPLVKKAALLGG
                     AFSVLAIIVTLIVSPALINSLFKGDSALLIGLLISIVAYYVMFLTRGTLSGNGRFGTY
                     GLLNGSEGGVRIAFVIALVILGASTPGPYGIALALPPLFAVGI"
     gene            863..2251
                     /gene="lpdA"
                     /locus_tag="VFX21_05300"
     CDS             863..2251
                     /gene="lpdA"
                     /locus_tag="VFX21_05300"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX5095405.1"
                     /translation="MPEEFDVVVLGGGPGGYAAALYGAAAGLSIAMVEADRVGGTCLN
                     RGCIPAKELLQTAEVMRTVQGAAEFGVTTTPGGIEMPVTQVRKQQVVDRLVNGLSGLL
                     KNRKVTVVAGRGTLAPDGHTVRVDDGTELHGRNVVLATGSAPRSLPLPGLDFDGTHVM
                     SSDHVLQLDHVPARVAVIGGGAIGCEFASFLTDVGAEVTILEALPQVLTGVDQQVAQT
                     VVRAFNKRGMKVQTGVKIISAERGAETVIRYEGKNGEEKLTVDLVVVSIGRRPLSENV
                     GYEEAGVKVDERGFVVVDGNMRTTVDGVYAVGDLVATPQLAHVAFAESIVAIKTMLGE
                     PALPIEYDKVPWGIYCHPEVGFCGLTEEQAKEQGYDVVTSVHRWMGNGRALIMGETDG
                     MVKIVADKDGPLLGVHIAGELATELIVEGYLSVNWEATAGDLGLLIHPHPSLSELFGE
                     SVLALTGRGLHN"
     gene            2278..>2408
                     /locus_tag="VFX21_05305"
     CDS             2278..>2408
                     /locus_tag="VFX21_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013735202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX5095406.1"
                     /translation="MPQLGETVTEGTITRWFKSVGEHIDADEPLFEVSTDKVDSEVPA
                     "
BASE COUNT          414 a          774 c          835 g          385 t
ORIGIN      
        1 cgatccccac cgcgaacaat ggcggcagcg cgagcgcgat tccgtacggg cccggtgtcg
       61 acgcaccgag gatgacgagc gcgatcacga acgcgatgcg cacgccgccc tcggagccgt
      121 tgaggagtcc gtacgtgccg aagcggccgt tgcccgacag cgtgccgcgt gtcaggaaca
      181 tgacgtaata cgcgacgatc gagatgagca gcccgatgag gagcgccgag tcgcccttga
      241 agagactgtt gatcaatgcc ggcgacacga tcaaggtgac gatgatcgcg agcaccgaga
      301 acgcgccgcc gaggagcgcc gccttcttga cgagtggtgc gccgccgcgc ccctgcgagt
      361 tccggtgcgc cagcgagcgc gcgacctctt gttcgagcgg ctggaacaag cccggcgtaa
      421 cgatgaagat caggatccag agcccgttca gcgccgcgta ctgcgactcg tcgagctggc
      481 ggaacgtgag gatctggaac ccgtaggcgg cgagaccggc gacgagcagg cccgcgccga
      541 ccgcgtaggt gccctcgggc agcgggctgt tcttggcggc atcgatcagc cgttgcgcgc
      601 gttgtgccac cgtctgtctg ccgatcctct cccagcgacg agcctggcga atcctacggc
      661 tgttcgtggc gatctcctga cttcacagat cggccgttcc gggaccgata ggactcagaa
      721 ggagcggtag cgttcgacga gagatgagta cccggaccct gctgattctc gccgccgtta
      781 ccgggctcgc gattctcgtc gcgttcgcgg tgcaagtcag cgtggtcgcc cgttgaatgc
      841 ccagttgaag gggtgagtga ccgtgcccga ggagtttgat gtcgtcgtac tcggcggcgg
      901 gcccggcgga tacgcagccg cgttgtacgg tgccgcggcg gggctctcga tcgcgatggt
      961 cgaggccgac cgcgtcggcg ggacctgcct gaaccggggc tgcatcccgg ccaaggagct
     1021 cctgcagacg gccgaggtga tgcgcacggt gcagggcgcg gccgaattcg gcgtgacgac
     1081 cactccgggt ggcatcgaga tgcccgttac ccaggttcgc aagcagcagg tcgtcgaccg
     1141 gctcgtcaac ggcctgtcgg gactgttgaa gaaccgcaag gtcaccgtcg tcgcgggccg
     1201 cggcaccctt gcccccgacg gtcacacggt gcgcgtcgac gacggcacgg agctgcacgg
     1261 tcgcaacgtc gtgctcgcga cgggttccgc gccgcggtcg ttgccgctgc cgggtctcga
     1321 cttcgacggc acgcacgtca tgtcgtccga tcacgtgctc cagctcgacc acgttcccgc
     1381 gcgggttgcc gtcatcggcg gcggcgcgat cgggtgcgag ttcgcctcgt tcctgaccga
     1441 cgtcggcgcg gaggtcacga tcctcgaagc cctcccgcag gtgctcacgg gtgtcgacca
     1501 gcaagtcgcg cagaccgtcg tgcgcgcgtt caacaagcgc ggtatgaagg tgcagaccgg
     1561 cgtcaagatc atctccgccg aacgcggcgc cgagacggtc atccgctacg agggcaagaa
     1621 cggtgaggag aagctcaccg tcgatctcgt cgtcgtgagc atcgggcggc ggcctctgag
     1681 cgagaacgtc ggttacgaag aagccggcgt caaggtcgac gagcgtggct tcgtcgtcgt
     1741 cgacggcaac atgcgcacga cggtcgacgg cgtctacgcg gtcggcgatc tcgtcgcgac
     1801 accgcaactc gcgcacgtcg cgttcgcaga gtcgatcgtc gcgatcaaga cgatgctggg
     1861 cgagcccgcg ctgcccatcg aatacgacaa ggtgccgtgg ggtatctact gccatccgga
     1921 ggtcgggttc tgcgggctga ccgaagagca ggccaaggag cagggttacg acgtcgtcac
     1981 ctcggtgcac cgctggatgg gcaacggccg cgcgctcatc atgggcgaga ccgacggcat
     2041 ggtgaagatc gtcgccgaca aggacggtcc gttgctcggc gtgcacatcg ccggtgagct
     2101 ggcaaccgag ctcatcgtcg agggctacct ctcggtcaac tgggaagcga ccgccggcga
     2161 cctcggtctg ctgatccatc cgcatccgtc gttgtcggag ctgttcggcg agtcggtgct
     2221 cgcgctgacc ggccgcggcc tgcacaacta gataggggac acgtcgtgga cgtcacgatg
     2281 ccgcaactcg gtgaaacggt caccgaaggc accatcacgc gatggttcaa gagcgtcggc
     2341 gagcacatcg acgccgacga gccgctgttc gaggtctcga ccgacaaggt cgactccgaa
     2401 gtgcccgc
//
LOCUS       DASUGA010000271         2263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_982441, whole genome shotgun sequence.
ACCESSION   DASUGA010000271 DASUGA010000000
VERSION     DASUGA010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982441"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1034
                     /locus_tag="VFX21_05310"
     CDS             135..1034
                     /locus_tag="VFX21_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095407.1"
                     /translation="MSTALGAVYDRGYRPYEGPRGRRGAATFALYKASIRRALGIRRS
                     WRQKVMPFVLLGIVTIPAIVNVGIGYVTRNELFESDRVEIITYREYVGVSSALLLFVA
                     LVAPDVICPDRRQHVLPLMFARPMTGVDYVLAKVGAIATILFAFSFIPQVVLFVGNML
                     VSDRALDYFTGHLEVLWKVPLAVLLLSLYYAVIGVALASLTDRRIVAGASIIGLFLVT
                     SITSGILVPDDYEFRGGSAAALINVLFMPLYLRDLVFLGHIDPDSPLTMVDHAGLLAV
                     ATYVAVLALGLGILLRRYRWVER"
     gene            1031..2011
                     /locus_tag="VFX21_05315"
     CDS             1031..2011
                     /locus_tag="VFX21_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5095408.1"
                     /translation="MSTADAVHDETFGDREYDPAFAPEATVEVSDVSVWFGPKVALSE
                     LSCSFGPGVTGLLGPNGAGKTTLMRALTGMIRVNQGSIRITGQNPRREREVFSRIALV
                     PEDEAVPGGLTARQFVRYVADLHRIPDRDAPDSTLRQVGLLDAADRSVEGFSKGMRQR
                     TKVAAALVNDPQVLVLDEPLNGADPVQRVHLITLFKALGAQGRTVIVSSHVLNEVERL
                     AERVIVLIHGRLAAAGGHHAIRDAMDDRPRHVLVRSDDLRRLASALISLESVGGVTFD
                     PHRDGLVIQTLQAREVATALARLARDNGVRLLEVRALDDSLESLFRELVR"
     gene            2008..>2263
                     /locus_tag="VFX21_05320"
     CDS             2008..>2263
                     /locus_tag="VFX21_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095409.1"
                     /translation="MSVPAPVAVPSSRAPRPRSRFVAILLYTFRSCFPRRRYFALLFP
                     CAGAVLFGLLALALDERTAERAFATVAADGIFGLVVPIAAL"
BASE COUNT          311 a          812 c          733 g          407 t
ORIGIN      
        1 gtgacgatcg accgcgacga gcagaacgac acgctgcggg acgtcatcgc cgacctcggg
       61 ctcccgctct accgcttgtc gtcgcgcctc acgtcgttgg acgaggtctt cctgcagcga
      121 gcggagggca gcgcgtgagc accgctctcg gcgccgtcta cgaccgcggc taccggccgt
      181 acgaaggccc gcgcggccgc cgcggtgcgg cgacgttcgc gctgtacaaa gcatcgatcc
      241 ggcgcgcgct cggcatccgc cggtcgtggc ggcagaaggt catgccgttc gtgttgctcg
      301 gcatcgtcac catcccggcg atcgtcaacg tcggcatcgg ctacgtcacc cgcaacgagt
      361 tgttcgagag cgaccgcgtc gagatcatca cgtatcgcga gtacgtcggc gtgtcgtccg
      421 cgctgctgct cttcgtggcg ctcgtcgcac cggacgtcat ctgcccggac cgccgccagc
      481 acgtactccc gctgatgttc gcgagaccga tgaccggcgt cgattacgtg ctcgcgaagg
      541 tcggtgcgat cgcaaccatc ctgttcgcgt tctcgttcat cccgcaggtc gtcctcttcg
      601 tcggcaacat gctcgtgagc gaccgggcgc tcgactactt cactggtcac ctcgaggtgt
      661 tgtggaaggt gccgctcgcg gtcttgctgc tttcgctgta ctacgcggtg atcggcgttg
      721 cgttggcgtc gctcaccgac cgccgcatcg tcgcgggcgc gtccatcatc ggcttgttcc
      781 tcgtgacgtc gatcacgtcc ggcatcctcg ttcctgacga ctacgaattc cgaggcggtt
      841 cggccgccgc actgatcaac gtgctcttca tgccgttgta cttgcgcgac ctcgtgttcc
      901 tcggccacat cgacccggac tcgccactca ccatggtcga ccacgccggc ctcctcgcgg
      961 tcgcgacata cgtcgcggtc ctcgcgctcg gcctgggcat cctgctgcgc cgctaccgct
     1021 gggtggagcg atgagcaccg ccgacgccgt gcacgacgag acgttcggcg atcgggagta
     1081 cgaccccgcg ttcgcacccg aagcgacggt cgaggtctcc gacgtgtccg tgtggttcgg
     1141 ccccaaggtc gcactgtcgg agctgagctg ttccttcggt ccgggtgtca ccggcctgct
     1201 gggcccgaac ggcgcgggca agacgacgtt gatgcgcgcg ctgaccggca tgatccgcgt
     1261 caaccaggga tcgatccgca tcacgggcca aaacccacgc cgggagcgcg aggtgttcag
     1321 ccgcatcgcg ctcgttcccg aggacgaagc ggtgcccggc ggactcacgg ctcggcagtt
     1381 cgtgcgctac gtcgccgact tgcaccgcat ccccgatcgg gacgcacccg acagcacgct
     1441 gcgccaagtc gggctgctgg acgccgcgga ccgcagcgtc gaaggcttca gcaaaggcat
     1501 gcgccagcgc acgaaggtcg cggccgcgct cgtcaacgac ccgcaggtcc tcgtgctcga
     1561 cgaaccgctg aacggcgcgg atccggtaca gcgcgtgcac ttgatcacgt tgttcaaagc
     1621 gctcggcgcg caaggacgca cggtcatcgt gagctcgcac gtcctgaacg aggtcgagcg
     1681 gctcgccgaa cgcgtcatcg tgctcatcca cggccggctc gccgcggccg gcggtcacca
     1741 cgcgattcgt gacgcgatgg acgaccggcc gcgccacgtg ctcgtccgtt ccgacgacct
     1801 gcggcggctc gcatccgccc tcatctcgct cgaatcggtc ggcggcgtga ccttcgaccc
     1861 gcaccgcgac ggcctggtga tccagacgct ccaggcgcgt gaagtcgcga ccgcactcgc
     1921 ccggctggca cgcgacaacg gcgtgcggct cctcgaggtg cgcgccctcg acgactcgct
     1981 cgagagcctg ttccgggagc tggtgcgatg agtgtgcccg caccggtcgc cgtgccgtcg
     2041 tcgcgtgcgc cgcgcccgcg gtcgcgcttc gtcgcgatct tgctctacac gttccggtcg
     2101 tgcttcccgc gccggcgtta cttcgcgctg ttgttcccat gtgcgggcgc tgtgttgttc
     2161 ggcttgctcg cactggccct cgacgaacgt acagccgagc gcgcgttcgc aaccgtcgcc
     2221 gccgacggca tcttcgggct cgtggtgccg atcgcggcgc tca
//
LOCUS       DASUGA010000272         7023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1551413, whole genome shotgun sequence.
ACCESSION   DASUGA010000272 DASUGA010000000
VERSION     DASUGA010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551413"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VFX21_05325"
     CDS             <1..759
                     /locus_tag="VFX21_05325"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HEX5095410.1"
                     /translation="EGFIARGGNAAFFSGNTSFWQVRLEGPTVEGPAATIVGYKGQFK
                     QDPVLGTERVPELTSMWSDHLIERPENHMTGVSFARGGYHRIGRRATAGAGGYTIHRP
                     HHWIFDGTDIGYGDVLGAAAVTVGYECDGCDFTYRDGLPYPTGRDGTPDNFEILGTAP
                     AAHFSRTTSVRPTPPDQPSEVEFIAARLFGARSDENVALIEHGHAVLGTYVAPSGGVV
                     VTSGSTDWAHGLAGRDAQVEQITRNILDRLGRKL"
     gene            777..1745
                     /locus_tag="VFX21_05330"
     CDS             777..1745
                     /locus_tag="VFX21_05330"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX5095411.1"
                     /translation="MAAVVASALAFAACSSGDDDDAASTSAPSTTAAPTTTTTAAVNA
                     STGCGTTPAVKGLVGERPGDVELSFDSGGVARTYRLAVPDSYDADMPVPVILNLHGSG
                     SNAIQQSVYSDLPRRAAERGVITITPDAIDGAWELAATGTDDDFLVALVRDVTDHYCI
                     DLDRVYTAGISLGSWKATQEACTHPDIFAAIALVAEEVRPPDCAPMPVVAFHGTGDPV
                     VPYGEGADPGITVTGFNGGLPGASVNMPSWAEGGGCGERQITPIGDDVEQWTYPDCDD
                     GIDVVFYSIKGGGHTWPGSDINVGATTQTIDATDIALDFFAAHPMR"
     gene            complement(1798..2346)
                     /locus_tag="VFX21_05335"
     CDS             complement(1798..2346)
                     /locus_tag="VFX21_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018548510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX5095412.1"
                     /translation="MSTLTARPNTALLVVDVQNDVVANAYRRDEVIAAINAAIDKARA
                     AGVPVIWVQHSDDELVRDSDGWQYVAELQRSDGEPLVHKHYNDSFEETTLEAELAQRG
                     VGHVVVSGAETDACVRATIHGALVRGYDTTLVGDAHTTNDYTSYGMPAPDLVIAHTNG
                     YWQYQSAPGRTAAVVPAAEITF"
     gene            complement(2343..2747)
                     /locus_tag="VFX21_05340"
     CDS             complement(2343..2747)
                     /locus_tag="VFX21_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006538325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5095413.1"
                     /translation="MDDVEVIKQLKARYFRTMDTKDWDGMRRVFTDDLVIDTSESGGG
                     VVDGADEFMAFLRETLADVVTVHHGHMPEITLTSPTTASGIWAMEDMLRWPNGMELHG
                     YGHYHETYERVGDEWRIKTSTLTRLRMDFTNP"
     gene            complement(2779..3546)
                     /locus_tag="VFX21_05345"
     CDS             complement(2779..3546)
                     /locus_tag="VFX21_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019929155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5095414.1"
                     /translation="MDLGTTNLRFEREGPIAWCVIDRPNARNALTPAMYNGVKRAVAL
                     VNRDPHLAALIITGTGDVFAPGGDLGGRSEPGDEMPPDWGMDILPFLDIRNSRAPVIS
                     AVNGICQAGGLLIAMLSDIAVASDRATFRVPELLRGIPDAFYASTLPAHVGVAVARDL
                     MLSGRPFDATEAQRLGVISRVVPHDDLRDAALEAARQVLRTPPDARTHVKRMLNERYG
                     HVDIQTMAWAVSHSPEMQEGMLAFMEKRNPSWMPPDL"
     gene            complement(3558..3959)
                     /locus_tag="VFX21_05350"
     CDS             complement(3558..3959)
                     /locus_tag="VFX21_05350"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX5095415.1"
                     /translation="MSTPYPRADPVDAAEAEFWSYVAGGELRVQRCVHCGEFRHPPRL
                     ACAACGSGAVEWVVASGHGEVWAATMIHPPTLPAFAPRTPYNAVVVRLVEGVFMVGNV
                     VDADDVPLGTRVDVEITEVEPGYRLPLFRRA"
     gene            complement(3956..5149)
                     /locus_tag="VFX21_05355"
     CDS             complement(3956..5149)
                     /locus_tag="VFX21_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095416.1"
                     /translation="MYGARQAAVAGIGQTQYAKDMGRTELDLACEAIRAACADAGLDV
                     ASVDGFVSYHIEQVAEVDLMGALGLAELRVMARTPSGGGGAASILGLAARAVECGDAE
                     CVIAFRARNRSKAASYGADPNQGGRPWAKGGDRLRDARQWHHPFGVAAPAHEFALIAS
                     RHMHVYGTRPEHFGMQAVAQRFHASRNPDAIMRKEITLDDWRASRPIADPIRLFDCSL
                     ENDGAAAVLVTSLDRARDLRPPAVPVLAQAQYGAPIHTQLCEFFASTAAYGERDGGAV
                     TAGRRLFAEAGLTPADVDVALFVEPFTVAVPLALEQYGFCGRGEGGPFIESGATRWPG
                     GALPVNTHGGSNGEAFVHGVNHLPEAVRQLRGTAVNQVAGAEVAFVCGAISDPSGAVL
                     LGVDR"
     gene            5204..6427
                     /locus_tag="VFX21_05360"
     CDS             5204..6427
                     /locus_tag="VFX21_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011606376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5095417.1"
                     /translation="MELPKIISVDDHVVEPAHVWQTWLPEKHRAKGPRVERKRWGKFR
                     LKKGAKYEMTEDPEGEWGDAWIYEDKVIYVQKKFVAIPKSATKNDDPSTFDKTVMTMT
                     ATTYDDMRPGCWDPKERKKDFELNWVDGSLPFPTFPRFCGQTFKEADDKELALACVQA
                     YNDWMVEEWCDPSIGVNIPLCIMPLWDVELAVKEIERNAARGVRAVCFSELPTRLDLP
                     SIHTGHWDPMFAACNDTGTTVCMHVGSSSSDPASSKDAPPGVGSMVAFNNSMASLGDY
                     LFSGKMAQFPKLKIAYSEGQIGWLPYALERADTVWVQHEAWLHSQRLCPEPPSTYYYD
                     RVFGCFTWDHHGVKSLEEVGENNICFETDYPHTDTTWPNSKEYCEKILDGLTDEQKYK
                     ILRGNAIRMLELDRV"
     gene            6443..>7023
                     /locus_tag="VFX21_05365"
     CDS             6443..>7023
                     /locus_tag="VFX21_05365"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5095418.1"
                     /translation="MSVIVVTGAAQGMGRACVDRLRAEATHLVAVDLDAPDIPGTVGV
                     ACDVSDPAAIARLVDEVRGLGLFRALVHAAGISPTMADSRRVMEVDLVGTQLLLDAFE
                     PLVEDGSSAVCFASSSAHMIALMGPNPELDALVEQPLADDLLDRATALLPDPGIAYAW
                     AKRGVIRAVARAAVTWGRRGGRTNSVSPGIIDTG"
BASE COUNT         1161 a         2451 c         2341 g         1070 t
ORIGIN      
        1 gaaggattca tcgcccgcgg cggcaacgcc gcgttcttct cgggcaacac gtcgttctgg
       61 caggtccgcc tcgagggacc caccgtcgaa ggacccgcgg cgacgatcgt cggctacaag
      121 ggccagttca aacaggatcc ggtgctcggc accgaacgcg tccccgagct cacgagcatg
      181 tggtcggatc acctcatcga gcggcccgag aaccacatga ccggcgtgag cttcgcgcgc
      241 ggcggctatc accggatcgg ccggcgcgcg accgccggtg ccggcggcta cacgatccac
      301 cgcccgcacc actggatctt cgacggcacc gacatcggct acggcgacgt gctcggcgca
      361 gccgcggtca ccgtcggcta cgagtgcgac ggctgcgact tcacctaccg cgacggcctg
      421 ccgtatccga ccggccgcga cggcaccccc gacaacttcg agatcctcgg caccgcgcct
      481 gccgcgcact tctcgcgcac gacttccgtg cggccgacgc cgcccgacca accatcggaa
      541 gtcgagttca tcgcggcgcg attgttcggc gcgcggtcgg acgagaacgt cgcgctcatc
      601 gagcatggcc acgcggtgct cggcacctat gtcgcgccgt cgggtggggt cgtcgtgacg
      661 tccggcagca ccgactgggc ccacgggctc gccggacgcg acgcgcaggt cgaacagatc
      721 acccgcaaca tcctcgaccg gttgggacgg aagctgtgag gttgcgaaga cttgccgtgg
      781 cggcggtggt cgcgtcggcg ctggcattcg cggcgtgctc gtcgggtgat gacgacgacg
      841 cggcgtcgac gagcgcaccc tcgaccaccg ccgcgccgac aacgacgacg acggcagcgg
      901 tgaacgcaag taccggctgc ggtaccacgc ccgcggtcaa ggggctggtg ggcgaacgtc
      961 ccggcgacgt cgagctgagc ttcgacagcg gcggcgtcgc gcgcacctac cgcctcgcgg
     1021 tgcccgactc ctacgacgcc gacatgcccg tcccggtcat cctcaacctg cacggttcgg
     1081 gatcgaacgc catccagcag agtgtctaca gcgatctgcc ccgacgggcc gccgagcgcg
     1141 gtgtgatcac gatcaccccc gatgccatcg atggcgcgtg ggagcttgca gccacgggta
     1201 ccgacgacga cttcctcgtc gcgctcgtgc gcgacgtgac ggaccactac tgcatcgatc
     1261 tcgatcgtgt gtacacggcc ggaatctcac tcggctcgtg gaaggcgacg caggaggcat
     1321 gcacccatcc cgacatcttc gccgcgatcg cgttggtcgc cgaagaggtc cggccccccg
     1381 attgcgcgcc gatgccggtc gtcgcgtttc acgggaccgg cgacccggtt gtgccgtacg
     1441 gcgagggcgc ggacccgggc atcacggtca ctggcttcaa cggcggattg cccggcgcgt
     1501 cggtcaacat gccgtcgtgg gcagaaggcg gcggctgcgg cgagcgccag atcacaccga
     1561 tcggcgacga cgtcgagcag tggacgtacc cggactgtga cgacgggatc gacgtcgtct
     1621 tctactcgat caagggcggc ggccacacgt ggccgggctc ggacatcaac gtcggcgcga
     1681 cgacgcagac gatcgacgcg accgacatcg cgctcgactt cttcgcagcc cacccgatgc
     1741 gctgacagcg cgtttgaacg atgaccgccc ttgtagggca cgctcgcacg caaacaccta
     1801 gaacgtgatc tcggccgcgg ggacgacggc ggcggtgcgc ccgggcgcgc tctgatactg
     1861 ccagtagccg ttggtgtggg cgatgacgag gtcgggcgcg ggcatgccgt acgacgtgta
     1921 gtcgttcgtc gtgtgcgcat cgccgacgag cgtcgtgtcg tagccgcgca cgagggcgcc
     1981 gtgaatcgtc gcgcgtacac aggcgtcggt ctcggcgccg gacacgacga cgtggccaac
     2041 accgcgctgt gcgagctctg cttcgagtgt cgtctcctcg aacgagtcgt tgtagtgctt
     2101 gtgcaccaag ggctcgccgt cggagcgctg caactccgcg acatactgcc agccgtcact
     2161 gtcgcgcacg agctcgtcgt cggagtgctg cacccagatc accggcacgc cggccgcgcg
     2221 cgccttgtcg atcgcggcgt tgatcgccgc gatgacctcg tcgcgacggt aggcgttggc
     2281 gacgacgtcg ttctggacgt cgacgaccag cagtgcggtg ttcggacggg cggtcaaggt
     2341 gctcatgggt tcgtgaagtc catccggagt cgcgtgagcg tcgacgtctt gatacgccat
     2401 tcgtcgccga cgcgttcgta ggtctcgtga tagtgcccgt acccgtggag ctccatgccg
     2461 ttgggccagc gcagcatgtc ctccatcgcc catatcccac tggccgtcgt cggcgacgtc
     2521 aaggtgatct ccggcatgtg accgtggtgc accgtcacga cgtcggcgag cgtctcgcgc
     2581 aggaacgcca tgaactcgtc ggcaccgtcg acgacaccgc ccccggactc cgacgtgtcg
     2641 atgacgaggt cgtcggtgaa cacacggcgc atgccgtccc aatccttcgt gtccatagtg
     2701 cggaagtagc gcgccttcag ctgcttgatc acctcgacgt cgtccatgga cccaacgtag
     2761 tcattcggtg cgcagcacct acaggtcggg cggcatccag ctcgggttgc gcttctccat
     2821 gaacgcgagc atgccttcct gcatctcggg cgagtgggaa acggcccacg ccatcgtctg
     2881 gatgtcgacg tgcccgtagc gttcgttcag catccgcttc acgtgggtgc gcgcgtcggg
     2941 cggcgtgcgc agcacctgcc gcgccgcttc gagcgcggca tctcgcagat cgtcgtgcgg
     3001 tacgacacgc gagatcacac cgaggcgttg cgcctccgtc gcgtcgaacg gtcgccccga
     3061 caacatcaag tcgcgcgcca ccgcaacacc gacgtgcgcg ggcagcgtcg acgcgtagaa
     3121 cgcgtcggga atgccgcgca agagctcggg cacgcggaac gtggcgcgat cactcgccac
     3181 cgcgatgtcg gagagcatcg cgatcaacag tccaccggcc tggcagatgc cgttcaccgc
     3241 ggagatcacc ggcgcgcggc tgttgcggat gtcgaggaac ggcaggatgt ccatgcccca
     3301 gtcgggcggc atctcgtcgc cgggctccga ccgtccgccg aggtcaccgc ccggcgcgaa
     3361 gacgtcgccg gtgccggtga tgatcagtgc cgcaagatga ggatcgcggt tgacgagcgc
     3421 gaccgcgcgc ttgaccccgt tgtacatcgc cggcgtcagc gcattgcgag cgttgggccg
     3481 gtcgatcacg caccacgcga tcggcccttc ccgttcgaac cggagattcg tcgttccgag
     3541 gtccatggct gcgcacccta cgcgcggcgg aacaacggca accgatagcc gggctcgacc
     3601 tcggtgatct cgacgtcgac gcgcgtgccg agtggcacat cgtcggcgtc gacgacgttg
     3661 ccgaccatga agacgccttc gacgaggcgc acgacgaccg cgttgtaggg ggtccgcggc
     3721 gcgaacgcgg gcagcgtcgg cggatggatc atcgtcgccg cccacacttc gccgtgaccc
     3781 gacgcgacga cccactcgac cgcgcccgat ccacaggccg cacacgcgag ccgcggcgga
     3841 tgccggaact cgccgcagtg cacgcaccgc tgcacccgca gctcgccacc ggcgacgtac
     3901 gaccagaact cggcctcggc cgcgtcgacc ggatcggcac gcggatacgg cgtgctcacc
     3961 ggtcgacccc gagcagcacc gcgcccgacg gatcgctgat cgcgccgcac acgaacgcga
     4021 cttccgcgcc cgcgacctgg ttcaccgcgg tgccgcgcaa ctgacgtacg gcctcgggga
     4081 ggtggttcac gccgtggacg aacgcctcac cgttcgatcc gccgtgcgtg ttcacgggca
     4141 gcgcgccgcc gggccagcgc gtcgcgccgc tctcgatgaa cggaccaccc tcgccgcgcc
     4201 cgcagaatcc gtactgctcg agcgcaagcg gcaccgccac ggtgaatggc tccacgaaca
     4261 gcgccacgtc gacgtccgcc ggtgtgagac cggcttctgc gaacagccgg cggccggcgg
     4321 tgaccgcgcc gccgtcgcgt tcgccgtacg ccgcggtcga cgcgaagaac tcgcacagct
     4381 gtgtgtggat cggtgcgccg tactgcgcct gcgcaagcac cggcaccgcc ggcggccgca
     4441 ggtcgcgcgc ccgatccaac gaggtcacca agaccgcggc cgcaccgtcg ttctcgagtg
     4501 agcagtcgaa caaccggatg ggatcggcga tcggccgcga cgcacgccag tcatcgaggg
     4561 tgatctcctt gcgcatgatc gcgtcgggat tgcggctcgc gtgaaaccgt tgcgcgaccg
     4621 cttgcatccc gaaatgctcc ggtcgcgtgc cgtagacgtg catgtgccgg ctcgcgatca
     4681 acgcgaactc gtgcgcgggc gcggcgacac cgaacgggtg atgccactgg cgtgcgtcgc
     4741 gcaggcgatc gccgcctttc gcccacggcc gtccaccctg attcggatcg gcgccgtacg
     4801 acgccgcctt cgaacggttg cgagcccgga acgcgatgac gcactccgcg tcaccgcact
     4861 cgaccgcgcg cgccgcgagc ccgagaatcg acgccgcgcc gccaccaccc gacggcgtgc
     4921 gcgccatgac gcgcagctcg gccagaccga gtgcgcccat gaggtcgact tcggcaacct
     4981 gctcgatgtg gtagctgacg aacccgtcga cggacgcgac gtcgagcccc gcgtccgcgc
     5041 acgcggcgcg gatggcttcg cacgcgaggt cgagctcggt gcggcccatg tccttcgcgt
     5101 actgcgtctg gccgatcccg gccaccgccg cctggcgcgc cccgtacatc ccgggggtac
     5161 tgtaaacctg acacgcacgt catatacgag ccccggggtc cgcatggaac tgccgaagat
     5221 catcagcgtc gacgaccacg tggtcgagcc cgcgcacgtc tggcagacgt ggctgccgga
     5281 gaagcaccgc gccaagggcc cgcgcgtcga gcgcaagcgc tggggcaagt tccgcctcaa
     5341 gaagggcgcc aagtacgaga tgaccgagga tccagaaggc gaatggggcg acgcctggat
     5401 ctacgaggac aaggtcatct acgtgcagaa gaagttcgtc gcgatcccga aatcggcgac
     5461 gaagaacgac gacccgtcga cgttcgacaa gacggtcatg accatgaccg cgacgacgta
     5521 cgacgacatg cgaccgggat gctgggaccc gaaggagcgc aagaaggact tcgagctcaa
     5581 ctgggtcgac ggctccttgc cgttcccgac gttcccgcgc ttctgcggcc agacgttcaa
     5641 ggaggccgac gacaaggagc tcgcgctcgc ctgcgtgcag gcgtacaacg actggatggt
     5701 cgaggagtgg tgtgatccct cgatcggcgt gaacatcccg ttgtgcatca tgccgctctg
     5761 ggacgtcgag ctcgcggtca aggagatcga acgcaacgcg gctcggggcg tgcgcgccgt
     5821 gtgcttctcc gagctgccga cccggctcga cctgccgagc atccacaccg gccactggga
     5881 cccgatgttc gcggcgtgca acgacaccgg cacgaccgtg tgcatgcacg tcggttcgtc
     5941 gtcgagtgat ccggcgtcgt cgaaggacgc gccgccgggc gtcggcagca tggtggcgtt
     6001 caacaactcg atggcgtcac tgggcgacta cctgttcagc gggaagatgg cgcagttccc
     6061 gaagttgaag atcgcgtact ccgaaggcca gatcggctgg cttccctatg ccctggaacg
     6121 cgccgacacc gtctgggtcc agcacgaggc gtggctgcac tcgcagcgcc tgtgcccgga
     6181 gccgccgtcg acgtactact acgaccgcgt gttcggctgc ttcacgtggg accaccacgg
     6241 cgtgaagtcg ctcgaagaag tcggcgagaa caacatctgc ttcgagaccg actacccgca
     6301 caccgacacg acgtggccca actcgaagga atactgcgag aagatcctcg acggcctcac
     6361 cgacgagcag aagtacaaga tcctgcgcgg caacgcgatc cggatgctcg agctcgacag
     6421 agtttgagaa gatgcgctcg atatgagcgt catcgttgtc accggtgctg cgcagggcat
     6481 gggccgcgcc tgcgtcgacc gtttgcgtgc cgaagcgaca catctcgtcg cggtcgacct
     6541 cgacgcgccg gacatccccg gcacggtcgg cgtcgcctgt gatgtctccg atccggccgc
     6601 gatcgcgcgc ctcgtcgacg aagtacgcgg gctcgggctg ttccgcgcgc tcgtgcacgc
     6661 ggcgggcatc tcgccgacga tggccgactc gcgtcgcgtg atggaggtcg acctcgtcgg
     6721 cacgcagttg ttgctcgacg cgttcgaacc gctcgtcgag gacgggtcga gcgccgtgtg
     6781 cttcgcctcg agctctgcgc acatgatcgc gttgatgggc ccgaatcccg agctcgacgc
     6841 gctcgtcgag caaccactcg cggacgacct gctcgaccgg gccaccgcgc tgcttcccga
     6901 ccccggcatc gcctacgcgt gggccaagcg cggcgtcatc cgcgcggtgg cgcgtgccgc
     6961 ggtcacatgg ggtcgtcggg gcgggcgcac caactccgtg tcgcccggca tcatcgacac
     7021 cgg
//
LOCUS       DASUGA010000273         4064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1514841, whole genome shotgun sequence.
ACCESSION   DASUGA010000273 DASUGA010000000
VERSION     DASUGA010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514841"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            468..689
                     /locus_tag="VFX21_05370"
     CDS             468..689
                     /locus_tag="VFX21_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095419.1"
                     /translation="MAKPVKFEHYRYVGDKRNQVVYDLDLYDSDPAVKALVDELMESE
                     QFLGISPQTLAEARNRNYRPHKSIKEEDE"
     gene            complement(690..1292)
                     /locus_tag="VFX21_05375"
     CDS             complement(690..1292)
                     /locus_tag="VFX21_05375"
                     /inference="COORDINATES: protein motif:HMM:NF020398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HEX5095420.1"
                     /translation="MTTQTTNPAAPALKEWAAIVHALLEGEQIVDVRKGGIKEDGRHF
                     DLPARRVWLSPTAEHQKPELLKPAYAHWVDLADASPVGGPVTMQGWADVVDVATITEP
                     EHVEALDSKLIWTRDYVESRFNWKKRDPLWVLVLRVHRLAEPVTIGWRDEYGGCTSWV
                     NYDGAPADPASLPSELVLSDVAFEAKHKGIREALPAECWQ"
     gene            1315..2058
                     /locus_tag="VFX21_05380"
     CDS             1315..2058
                     /locus_tag="VFX21_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5095421.1"
                     /translation="MEEIVSEGLRLGAHFARPNGMTRVPALLVLPGFPRGPGGAASLG
                     NTDQTLCDRIARESGWAGMTITFRGTGTSEGDFSIEGWLADVRAAVDVLHARTDTTGV
                     WIAGFRLGGTLAIMTAADDERVRGIATFAAPASLRTWVRDPDWFLEYARRTGVLRTAD
                     YPADPASWIRAISNLDPVEAAKRIDPRPWLLMHGSADDVVPVDDARVLEDASGGHAEV
                     RIIANGPHRLRHDPRAIAGLLGWLDRQEP"
     gene            complement(2075..2971)
                     /locus_tag="VFX21_05385"
     CDS             complement(2075..2971)
                     /locus_tag="VFX21_05385"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX5095422.1"
                     /translation="MKVLITGATGFVGPYLIDECTKHGDDVVDAGDATTGFDIADRDS
                     CFATFEKFEPDAVYHLAALSHVGDSWADPTPCLRVNIEGTANVLDASAKIGAQRVLLI
                     GSSEEYGKVSDPTTPVKEDEPLRPLSPYGASKVAASYLGVQAFYARNLPVVRVRAFGH
                     TGPGQSPKFVVPALAHRIAEAERTGANEIVMGSLTPVRDLSDVRDVVRAYRLLLTQGE
                     PGEVYNVGRGVGSTIGDIAKSLIAQSTATLEIVTDPALVRPVEMPGLIADTSKIRAAV
                     GWEPEHSLDDTLASMLDAARNS"
     gene            complement(2968..>4064)
                     /locus_tag="VFX21_05390"
     CDS             complement(2968..>4064)
                     /locus_tag="VFX21_05390"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX5095423.1"
                     /translation="RPVGAGVYTIAIARGLAAREGVELHLVTRRSDAERWPAVAAGAV
                     VHPVAPDRRPVRLAWEQMGARAVIGDVAPDVWHGPHYTMPLRASVPTVVTIHDLTFFD
                     HPEYHERSKVVYFRQMIRLAAKRASVLICVSEYTAARLRALCAPKGDVVVVPHGVDHA
                     QFHPGGDEAADLAALAAHGIAPPYLAFAGTFEPRKNLPALVQAFARCAVAHPEVRLVL
                     AGSDGWGATEARNAIATSGVATRILRPGYLAGDALTALFRRAEAVVYPSIEEGFGLPA
                     LEAMACGTPLVTSIGTALGEVAGDAALLVPPTDTDALAAAITTALDDPATAARLRAAG
                     PVRAAAFTWDASVEGHASAYERAVGASMRG"
BASE COUNT          658 a         1409 c         1394 g          603 t
ORIGIN      
        1 cggtgatcga aacccagctc gagtacctcc gggccatcgg cgccgtcggg cccgtcgtgg
       61 acggcgacga cgacccgccg ccccgggcgt cctgagcgct gatcacaccg cggatacgaa
      121 aagcggtatt cggaccccga acgatctggt acaaaggtcg gaccggagaa gggaggtggt
      181 ccagcacatg cacgataaac ctgggacccc ggaggtgcgc ggccgctagg ccgtcgagaa
      241 tccgactgga ccacctggcg gagtccaaac cgccgcaccg ccgtcgaggc tgccggacgt
      301 ggtcccaccg gcacaccaag gcttcggcgg atccgaatca gtcatggaac gaggggtgcg
      361 caagcgcccc tcgtttcacg ttcagggaga cgacagcgca gcgaccggcg gggagcggag
      421 tgtggagtcg acccatgatc atgcgttggg gcggtagctt ctgcgccgtg gccaagcccg
      481 tgaagttcga gcactaccgc tacgtcggcg acaagcggaa ccaggtcgtg tacgacctcg
      541 acctctacga ctcggatccg gccgtcaaag cgctcgtcga cgagctcatg gaatccgagc
      601 agttcctcgg gatctcaccg cagacgctcg ccgaagcgcg caaccggaac taccgccccc
      661 acaagtcgat caaagaagaa gacgagtagc tactgccagc actccgcggg cagcgcctcg
      721 cggatcccct tgtgcttcgc ttcgaacgcg acgtccgaca gcacgagttc ggacgggagc
      781 gacgcggggt cggcgggcgc cccgtcgtag ttcacccacg acgtgcagcc gccgtattcg
      841 tcgcgccatc cgatcgtgac cggctccgcg agccggtgca cccggagcac gagcacccac
      901 agcgggtcgc gcttcttcca gttgaaccgg gactcgacgt agtcgcgcgt ccagatcagc
      961 ttggagtcga gcgcctcgac gtgttcgggc tcggtgatcg tcgcgacgtc gaccacgtcg
     1021 gcccatccct gcatggtgac gggaccgccg accggcgacg cgtccgccag gtcgacccag
     1081 tgggcgtacg cgggcttcaa cagctccggc ttctggtgct cggccgtcgg cgagagccac
     1141 acccgccgcg cgggcaggtc gaagtggcgg ccgtcctcct tgatgccgcc cttgcggacg
     1201 tcgacgatct gttccccttc gagcaaggcg tgcacgatgg cggcccactc cttgagtgcc
     1261 ggggcggccg ggttcgtcgt ctgcgtggtc acgcaggcca ggataacgac ctacatggaa
     1321 gaaatcgtct ccgaaggact gcgcctgggc gcgcacttcg cgcggccgaa cgggatgacg
     1381 cgcgtgcccg cgctgctcgt cctgcccggc tttccgcgcg ggccgggcgg agccgcgtcg
     1441 ctcggcaaca ccgaccagac gctgtgcgac cgaatcgcac gcgaatcggg ctgggccggg
     1501 atgacgatca cctttcgcgg cacaggcaca tcggaaggcg acttttcgat cgaaggctgg
     1561 ctcgcggacg tgcgcgccgc ggttgacgta ttgcacgccc gtaccgacac caccggtgtg
     1621 tggatcgccg ggtttcgcct cggcggcacg ctcgcgatca tgaccgccgc cgacgacgaa
     1681 cgggtgcgcg gcattgcgac gttcgcggca ccggcgtcac tgcgcacgtg ggtgcgcgat
     1741 ccggactggt tcctcgaata cgcgcgccgc acgggtgtgc tgcgcaccgc ggactatccc
     1801 gccgatcccg cgagctggat tcgcgcgatc tcgaatctcg atccggtcga agcggcaaag
     1861 cgcatcgacc cgcgaccgtg gttactgatg cacggcagcg ccgacgacgt cgtgccggtc
     1921 gacgatgcgc gcgtcttgga agacgcgagc ggcgggcacg ccgaagttcg catcatcgcg
     1981 aacggtccgc accgcttgcg gcacgacccg cgcgccatcg cgggactgct cggctggctc
     2041 gaccgccaag aaccctagaa aacgaagagc gcctctagga attgcgggcg gcgtcgagca
     2101 tcgacgccag cgtgtcgtcg agcgaatgtt cgggttccca acccaccgca gcgcggatct
     2161 tgctcgtgtc ggcgataagg cctggcatct cgaccggccg cacgagcgca gggtcggtga
     2221 cgatctcgag cgttgcagtc gattgtgcga tcagcgactt ggcgatgtca ccgatggtcg
     2281 aaccgacacc gcggccgacg ttgtagacct cgcccggctc gccctgcgtg agcagcagcc
     2341 ggtacgcgcg cacgacatcg cgcacgtcgc tcaggtcgcg cactggcgtc agcgaaccca
     2401 tcacgatctc gttggcgccg gtgcgctccg cttccgcgat ccggtgcgcg agcgcgggca
     2461 ccacgaactt cggactctgt cccgggccgg tgtgcccgaa cgcgcgcacc cgcacaaccg
     2521 ggagattgcg cgcgtagaac gcttgcacgc cgaggtagga cgccgcgacc ttgctcgcgc
     2581 cgtagggact caacggccgc agcggctcat cttccttgac cggcgtcgtg ggatcgctga
     2641 ccttgccgta ctcctcggag ctgccgatga gcagcacgcg ctgcgcgccg atcttcgccg
     2701 acgcatcgag cacgttggcg gtgccctcga tgttcacgcg caggcacggc gtcgggtctg
     2761 cccacgaatc accgacatgg gacagcgccg cgaggtgata cacggcgtcg ggctcgaact
     2821 tttcgaacgt cgcgaagcac gagtcgcgat cggcgatatc gaagccggtc gtcgcatcgc
     2881 cggcatccac gacgtcgtcg ccgtgcttcg tgcattcgtc gatcaggtac gggccgacga
     2941 atcccgtcgc gccggtgatg aggaccttca tccccgcatg cttgcaccta cggctcgttc
     3001 gtacgcactt gcgtggcctt cgacggacgc gtcccaggtg aatgccgctg cccggaccgg
     3061 acctgcggct cgcaaccggg cggcggtcgc cgggtcgtcc agcgccgtgg tgatggcggc
     3121 ggccagcgcg tcggtgtcgg tcggcgggac gagcagcgcg gcgtccccgg ccacttcacc
     3181 cagcgccgtg ccgatgctcg tgacgagcgg tgtaccgcag gccatcgcct cgagggcggg
     3241 caggccgaaa ccttcctcga tcgagggata cacgaccgcc tcggcgcggc ggaacagcgc
     3301 ggtcagcgcg tcgcccgcga ggtagccggg ccgcaggatg cgggtcgcga cgccgctcgt
     3361 cgcgatcgcg ttgcgcgcct ccgttgcgcc ccagccgtcg ctgcccgcga gcacgaggcg
     3421 cacctcgggg tgggccacgg cgcaccgggc gaacgcctgc acgagcgcag gcaggttctt
     3481 gcgcggctcg aaggtgcccg cgaacgcgag atacggcggc gcgatgccgt gcgccgcgag
     3541 cgcggcaaga tcggcggctt cgtcgccgcc cggatgaaac tgcgcgtggt cgaccccgtg
     3601 cggcacgaca acgacatcgc ccttcggcgc gcaaagcgca cgcagccggg cggcggtgta
     3661 ttcgctgacg cagatcaaga ccgacgcgcg tttcgccgcc aggcggatca tctggcggaa
     3721 gtacacgacc ttgctgcgct cgtggtactc gggatggtcg aagaaggtga ggtcgtggat
     3781 cgtgacgacg gtcggaaccg acgcacgcag tggcatggtg tagtgcggcc cgtgccacac
     3841 gtcgggcgca acgtctccga tcaccgcgcg agcgcccatc tgttcccacg cgagtcgcac
     3901 aggccgccga tcaggagcga ccggatgcac gaccgcgccg gccgcgaccg ccggccagcg
     3961 ctcggcgtcg ctgcggcgcg tcaccagatg cagctccacc ccctcgcgcg cggcgaggcc
     4021 gcgtgcgatc gcgatcgtgt agacgccggc gccgaccgga cggt
//
LOCUS       DASUGA010000274         2529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_235803, whole genome shotgun sequence.
ACCESSION   DASUGA010000274 DASUGA010000000
VERSION     DASUGA010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235803"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VFX21_05395"
     CDS             <1..760
                     /locus_tag="VFX21_05395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX5095424.1"
                     /translation="DGFVVGERVLAIPTFGHGGFTECTIAPAATVFAIPDAMPFDAAS
                     ALHVTYQTGHLALHHRAHLQAGETLLVHAGAGGVGSAAIQLGVAAGARVFATAGGPEK
                     VDVCRKLGAELAIDYRADDFVTAIKDATGGRGVDVVYDPVGGDTYDRSTKCIAWEGRI
                     LVIGFTGGRFAEARTNHVLIKNYSVVGVHWGMYNLHDPALVGSIHDDLIALFEAGKIE
                     PLISERVSLAAVPAALTRLGGRGTWGKVVYSAGS"
     gene            complement(742..1713)
                     /locus_tag="VFX21_05400"
     CDS             complement(742..1713)
                     /locus_tag="VFX21_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5095425.1"
                     /translation="MRFVVYGAGAIGGVVGARLAQHGHDVLLVARGTHLDAIRAHGLR
                     LQAGTEDVTLPIPAAAHAREIDWREDDVVLLATKSQDTLGVALALAEDAPRSLPVVSL
                     QNGVANEPLLLRWFPNVYAVCVMSPTAHLEPGVVQAYSVPVSGLLDIGRYPHGLDDTA
                     RAVAAALASASFESIPRPDIMRWKYTKLLMNLGNAIEATCAPSEAMGALAKRVRNEGR
                     TVLRAAGIDAASSEEDRERRGSIMTITPIEGSVRGGGSSWQSLARGTGTIESDYLNGE
                     IVWLGRLHGVQTPANELVRETAVRAAREHLPPGSFDAHALLDQLPAE"
     gene            complement(1710..2501)
                     /locus_tag="VFX21_05405"
     CDS             complement(1710..2501)
                     /locus_tag="VFX21_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016570367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5095426.1"
                     /translation="MSNNALAGFEETQFTYNGKMRTVFRGGAGPGVVVIHEIPGITPL
                     VAAFGRRVVDEGFTVAMPVLAGTPGKAMSLPYVVSSFGPVCVSKEFRAWATNSTSPVI
                     EWLRALARDLHESAGGPGVGAVGMCFSGGFALAMSVDEAMRAPVLSQPSMPFPVGKKR
                     TRDLGISDADLAIVKARDDFCMMALRFSEDRAVPDARWKRYQEEFGDRLIAVDIDSSK
                     GNPHGIGKMSHSVLTEHLVDEPGHPTSDALERVLQFFKDRLKGGA"
BASE COUNT          394 a          882 c          855 g          398 t
ORIGIN      
        1 cgacggattc gtggtgggcg agcgcgtgct cgccattcct acgttcggtc acggcgggtt
       61 caccgagtgc acgatcgcgc ccgcggcgac ggtgtttgcc attccggacg cgatgccgtt
      121 tgatgctgcg tctgcactgc acgtcacgta ccagaccggt catctcgcgt tgcaccaccg
      181 cgcgcatctc caagcgggtg aaacgctgct cgtgcacgcg ggcgcgggcg gggtcgggag
      241 cgccgcgatc caactcggcg tggcggccgg ggcgcgcgtg ttcgcgaccg cgggtggacc
      301 ggagaaggtc gacgtctgcc gcaagctcgg cgccgagttg gcgatcgact accgcgccga
      361 cgacttcgtg accgcgatca aggacgcgac cggtggtagg ggtgtcgacg tcgtatacga
      421 cccggtcggc ggcgacacct acgaccgctc gacgaagtgc atcgcgtggg aaggtcgcat
      481 cctcgtcatc ggcttcacgg gcggccgctt cgccgaggcc cgtaccaacc acgtgctgat
      541 caagaactac agcgtggtcg gtgtgcactg gggcatgtac aacctgcacg accccgcact
      601 cgtgggcagc atccacgacg acctgatcgc actgttcgag gcgggcaaga tcgagccgct
      661 catctcggag cgagtgtcac tggccgccgt gcccgccgcg ctcacgcgtc tcggcgggcg
      721 gggcacgtgg gggaaggtcg tctactcggc cgggagctga tcgagcagcg cgtgcgcgtc
      781 gaacgaaccg ggcggcaggt gctcgcgcgc ggcgcgcacc gcggtctcgc gcacgagctc
      841 gttcgccggt gtctgcacac cgtggagacg accgagccag acgatctcgc cgttcaggta
      901 gtccgactcg atcgttcccg tcccgcgggc gaggctctgc cacgacgagc caccaccgcg
      961 gaccgaaccc tcgatcggtg tgatcgtcat gatcgagccc cgacgctcgc ggtcttcttc
     1021 gctcgacgcg gcgtcgatgc cggccgcgcg cagcacggtg cggccttcgt tgcgcacccg
     1081 cttggcgagg gcgcccatcg cctcgctcgg cgcgcacgtc gcttcgatgg cgttgcccag
     1141 attcatcagc aacttcgtgt acttccaccg catgatgtcg ggccgcggga tcgactcgaa
     1201 cgacgccgat gccagcgcgg ctgcaacggc ccgcgcggtg tcgtcgaggc cgtgcggata
     1261 acggccgatg tcgagcagcc cgctcacggg gacggagtac gcctgcacga cacccggttc
     1321 gagatgcgcg gttggcgaca tgacgcacac cgcgtagacg ttcgggaacc aacgcagcag
     1381 cagcggttcg ttggcgacac cgttctgcag tgagacgacc ggcaacgagc gcggcgcgtc
     1441 ctcggccagc gccagtgcga caccgagcgt gtcttgcgac ttcgtcgcca gcagcacaac
     1501 gtcgtcttca cgccagtcga tctcgcgcgc gtgcgcggcc gccggaattg gcagcgtcac
     1561 gtcttcggtg cccgcttgta ggcgcagccc gtgcgcacgg atggcgtcga ggtgcgtgcc
     1621 ccgcgcgacg agcaacacgt cgtggccgtg ctgggcgagc cgggcgccga cgacaccgcc
     1681 gatcgcaccc gcgccgtaga cgacgaacct cacgcgccgc ccttcaggcg gtccttgaag
     1741 aactgcagca cgcgctcgag cgcatcgctg gtcgggtgac cgggctcgtc gacgagatgc
     1801 tcggtcagta ccgagtgcga catcttgccg atgccgtgcg ggttgccctt cgacgaatcg
     1861 atgtcgacgg cgatcaaccg gtcaccgaac tcctcctggt agcgcttcca ccgcgcgtcc
     1921 ggtaccgcgc ggtcctcgga gaagcgcaac gccatcatgc agaagtcgtc gcgcgccttt
     1981 acgatggcga gatcggcgtc ggagatgccg agatcccggg tacgcttctt cccgacgggg
     2041 aacggcatcg acggctggct caagaccggc gcgcgcatcg cttcgtcgac cgacatcgcg
     2101 agtgcgaagc cgccgctgaa gcacatgccg accgcgccga cgcccggacc gccggccgac
     2161 tcgtgcaggt cgcgtgcgag cgcccgcaac cattcgatca cgggagacgt gctgttcgtc
     2221 gcccaggcgc ggaattcctt cgacacgcac acggggccga acgacgacac gacgtacggc
     2281 aggctcattg ccttgccggg cgtgcccgcc agtaccggca tcgcgacggt gaagccttcg
     2341 tcgacgaccc gccgcccgaa tgccgcgacc agcggagtga tgccggggat ctcgtggatg
     2401 accacgacgc ccggccccgc gccgccgcgg aagaccgtgc gcatcttccc gttgtacgtg
     2461 aactgcgtct cctcgaatcc cgccagtgcg ttgttactca tgcggcttcg gcctccggcc
     2521 ctcgatcac
//
LOCUS       DASUGA010000275         2544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1718269, whole genome shotgun sequence.
ACCESSION   DASUGA010000275 DASUGA010000000
VERSION     DASUGA010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718269"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFX21_05410"
     CDS             complement(<1..272)
                     /locus_tag="VFX21_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX5095427.1"
                     /translation="MTSHAEKAARFLALHHTDEVLLLPNAHDAGTAKVFEAMGFSATA
                     TTSAGFAGTLGRLDYHVTRDEALEHCAALVAATDLPVSADYENAFA"
     gene            663..1706
                     /locus_tag="VFX21_05415"
     CDS             663..1706
                     /locus_tag="VFX21_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX5095428.1"
                     /translation="MTSLTEPSLQVVGGVDTHKDVHVAAAIDTTGRMLGTVSFPTTSA
                     GYRALLGWLRGHGTVVRVGIEGTGCWGAGITRFVTDAKIPVVEVDRPNRQRRRRHGKS
                     DPTDAEAAARAALAHDATTIPKSRDGNVEMIRALRVARRSAIKARTQAANQLHALVVN
                     APDTLRAQLRTLNTNQLIEHAANFRAGSMTTPAAAIKLALHEIARRHRHLSGEIIRLD
                     AHLDELVKATAPQLVARRGVGTDTAGALLVATGDNPHRLANERSFAALCGASPRDASS
                     GKQQRHRLNRGGDRQANHALWRIVMVRMSCDPATKAYIARRRAEGKTTREAIRCLKRY
                     VAREIYQALHLTP"
     gene            complement(1789..>2544)
                     /locus_tag="VFX21_05420"
     CDS             complement(1789..>2544)
                     /locus_tag="VFX21_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5095429.1"
                     /translation="RDEKVPRNQGIIMLAVDVSASMTATDVQPSRIAAATVAARDFVE
                     SIPSGFEVGLVAFDASARVLSPPTDDHAKVAAAIDRLDTGEGTAAGEAIYAALNSISA
                     SLEDQAAADGNGNGNGDGREDLAATIVLLSDGTTTVGRPVEDAAQAADDAGVPITTIA
                     YGTDEGEVTIQGEVIPVPADTETMANVAETTGGSAFKAGSAEQLEGVYQDIQGRVGYT
                     TEQKEIGRWFMFVGILVLFAALAAAIVWMARLL"
BASE COUNT          409 a          916 c          809 g          410 t
ORIGIN      
        1 tcggcgaacg cgttctcgta gtccgcgctc accggcagat cggtcgcggc cacgagtgcc
       61 gcacaatgct cgagtgcttc gtcgcgcgtc acgtggtagt cgagccggcc gagcgttccc
      121 gcgaagcccg cgctcgtggt cgcggtcgcc gagaagccca tggcctcgaa gaccttggcc
      181 gtccccgcgt cgtgcgcatt cggcagcagc aacacctcgt cggtgtggtg cagggcgagg
      241 aaccgggcgg ccttctcggc gtgcgacgtc atgacatcca tctttgtcgc tctcgacctg
      301 ctcgcgtgag gcacgtctcg ttgttgtccg gcctcgagac tccagcgagt ggccgagacg
      361 gtcctgaggc ggcgcttcgc gcatcgcgag gacatcaacg gtgtgttgta agtccacccg
      421 ttggtggtcg ggtcggtcga ggatcggttc gaccttctcg ggatcggtct gactctcgca
      481 aggacatggg cttggtgcct tttcagtgat ctgtcgaccg gtctcgagag ggccgaacgc
      541 ggcaccagcc ggtccggacg gacgtgacct cataggagct tgaagcctct ccccttgatg
      601 tcttgtccgc cgtcccggcc ggcacgtgcc atccgagcac ctcttgagaa ggagccgatg
      661 acatgaccag cctgacagaa ccgagcctgc aagtggtggg cggcgtcgac acgcacaagg
      721 acgtgcacgt cgccgcggcg atcgatacga ccggacggat gctcggcact gtgtcgtttc
      781 ccacgacgtc cgccgggtat cgagcgttgc tgggttggct gcgcggacac ggcacggtcg
      841 tgcgcgtcgg gatcgaaggg accgggtgtt ggggcgccgg catcacgcga ttcgtcaccg
      901 atgccaagat tccggtggtt gaggtcgatc gcccgaatcg gcagcgacga cgtcggcacg
      961 gcaagtccga cccgaccgat gccgaggccg cggcgcgcgc ggcgctcgca cacgacgcaa
     1021 cgacgatccc gaaaagtcga gatggcaacg tcgaaatgat ccgcgcgttg cgggtcgcgc
     1081 gccgctccgc gatcaaagcc cgcacccagg ctgcgaatca gctccacgcg ctcgtggtca
     1141 acgcgcccga cacgctccgc gcgcagctgc gcaccctcaa cacgaaccaa ctcatcgagc
     1201 acgccgcgaa cttccgcgcc gggtcgatga ccacaccggc agccgcgatc aagctcgcgc
     1261 tgcacgaaat cgcccgccga caccgccacc tcagcggcga gatcatccgc ctcgacgccc
     1321 atctcgacga gctcgtgaag gcgaccgcac cccagctcgt cgctcgacgc ggcgtcggca
     1381 ccgacaccgc cggcgcattg ctcgtcgcca ccggcgacaa cccccatcga ctcgccaacg
     1441 aacgatcctt cgcggcgctt tgcggcgcct cgccccgcga cgcctcctcg ggcaaacaac
     1501 aacgccaccg cctcaaccgc ggcggcgacc gtcaagccaa ccacgcgctc tggcgcatcg
     1561 tcatggtccg catgagctgc gaccccgcga ccaaggccta tatcgcccgc cgccgcgccg
     1621 aaggcaagac cacccgcgaa gccatccgct gcctcaaacg ctacgtcgcc cgcgagatct
     1681 accaagccct ccacttgacg ccctaaacca ataggagcat cactgaggcc accgcctcgt
     1741 gcgacttctc gcgccgttgg atccctgtac ggcgcccgtg gatccctctt acagcaggcg
     1801 ggccatccag acgattgccg ccgcgagcgc cgcgaacaga acgaggatgc cgacgaacat
     1861 gaaccagcgt ccgatctcct tctgctcggt cgtgtagccg acgcggccct ggatgtcctg
     1921 gtagacacct tcgagctgtt ccgccgaccc ggccttgaac gcggagccac cggtggtctc
     1981 ggccacgttg gccatcgtct cggtgtcggc cggcaccggg atcacctcgc cctggatggt
     2041 gacctcaccc tcgtcggtgc cgtacgcgat cgtcgtgatc ggcacaccgg cgtcgtccgc
     2101 ggcctgggcc gcgtcctcga ccgggcggcc cacggtggtc gtgccgtccg agagcaacac
     2161 gatcgtcgcg gccaggtctt cgcggccgtc accgttgccg ttgccgttac cgtcggcggc
     2221 ggcctggtct tcgaggctcg cgctgattga gttgagcgcg gcgtagatcg cctcgcccgc
     2281 cgccgttccc tcgccggtat cgagccggtc gatcgccgcc gcgacctttg cgtggtcgtc
     2341 ggtcggcggt gagagcacac gggccgaggc gtcgaacgcg accaagccca cctcgaagcc
     2401 gctcgggatg ctctcgacga aatcgcgggc cgcaacggtc gcggcagcga tgcgcgacgg
     2461 ttgcacgtcg gttgccgtca tcgacgcgga cacgtcgacc gcgagcatga tgatgccctg
     2521 gttgcgcggc accttctcgt cgcg
//
LOCUS       DASUGA010000276         5533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1163228, whole genome shotgun sequence.
ACCESSION   DASUGA010000276 DASUGA010000000
VERSION     DASUGA010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163228"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VFX21_05425"
     CDS             complement(<1..334)
                     /locus_tag="VFX21_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095430.1"
                     /translation="MTVLPDLDDLVDLDWGIVDAGRKQLRDTGACEIPNFIRPEALDA
                     FIEDARAVAPLAHPSGGLGTVYLGFPDESFPADHPRQWLGNYRVQAVAYDLFPAESLV
                     RRLYEWQAL"
     gene            complement(331..1530)
                     /locus_tag="VFX21_05430"
     CDS             complement(331..1530)
                     /locus_tag="VFX21_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010987844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5095431.1"
                     /translation="MTSNYVVISTDCHAGGSLEQYKEFLETKWHNEFDAWRGAYKNPF
                     RDLSGSKRYRNWDSDARLNEQAEDGIVAEVAFPNTVPPFFPSASFMARAPQTAEEYER
                     RWAGLQAHNRWVRDHFVAAAPERRAGMAQILLNNVDDAVETVYWAAENGLRGGILLPG
                     VPPDSHLPPLYANDVYEPIWDALEETGLPVNHHSGSAAPDYGKYPAGGVLWLLETGFY
                     AHRAFWALIFGGVFERHPTLKFVLAESGMGWLADELPRTDAIAERIFGGKVGELGFTE
                     DQRLPMLPSEYFTRNVWVTASFMGPLECKRRHQVGLEKIMWASDYPHDEGTYPYSKQS
                     LRNTFHDVPENEVRMMLGETAAQLYNFDMAALQKLADEFGPSPEEIAQPIDQLPDSES
                     NAFSKMR"
     gene            complement(1560..2372)
                     /locus_tag="VFX21_05435"
     CDS             complement(1560..2372)
                     /locus_tag="VFX21_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095432.1"
                     /translation="MNLLTVTFVEMRRALHRRLVRWMVALALGMCAFVGVIVFNTSDN
                     AIELLQSPDHPANMYYWWYDHGQGFLTVAASFLAIGAAICGASVGGAEWRAGTITTVL
                     TWVPARLRLHFARTISAGILAFLISFGLQIVYLSAALPAVWANGSTDGTDGAWWSSLG
                     AAMVRVSFVSALIAMLALSIATIARNTAASLVALAVWALLVEGLIRGYKPHLARYLVT
                     ENVVTFVGWAQIDDVEFHRGPLTSMAALVTYAGIAIAAAAISFRRRDVTAAT"
     gene            complement(2369..3295)
                     /locus_tag="VFX21_05440"
     CDS             complement(2369..3295)
                     /locus_tag="VFX21_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5095433.1"
                     /translation="MPVIEIEGLRKEYRRFRGGTTVAVDGLHLEVPEGGVFGFLGPNG
                     SGKTTTIRCLLGLVRPSAGRMRLFGESTPAHLERVVTRIGAIVETPALFPTMSGRENL
                     RLLGALHGIGRARVEDVLAQVGLAERGDDKVRKYSLGMRQRLGLAAALLHDPALLVLD
                     EPANGLDPAGIREIREMLRGLAAEGRTVFVSSHLLAEVEQTCDRVAIIDRGRLLLDGR
                     VEDVLASAARPSLLVGLEDLAAGARALGAAGIACEPSEHLLRVAVAPADAALVTKTLA
                     DAGLYVTELRPETVSLEELFLQMTSHREDGAA"
     gene            3363..4547
                     /locus_tag="VFX21_05445"
     CDS             3363..4547
                     /locus_tag="VFX21_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5095434.1"
                     /translation="MTDLGYQAFDADNHYYEALDAFTRHLDPKLGPRCVQWCEIDGRK
                     YHVVGGRVSKAVVNPTFNPIALPGAMHDYFRGNPDKKQPWEFLAAREPIPAEYRDRDA
                     RIAKLDEFGLESVWLFPTLGMLYEELLKDDGEAVTLTFTAFNRWLEDDWGCNYRDRIF
                     AAPYISLCDIDWAIRELEWALDHDARTVVMRPAAPHTRDGQIPVSDPCFDPFWARVDE
                     AGITVVAHAGDSGYTSNGYARDGFSAAFSGGRWAPSIKSFHIEQAAFDFLITLVFDKL
                     FERFPNVRIASVENGSDFLRDLFKKLASTDRKMPGYFGTDPSDAFRRNIWVNPFWEDD
                     VHEVVDLMSADRVIFGSDWPHIEGMPRPLDYVAELKEFDDATQQLILRDNVRALNQRT
                     PA"
     gene            4544..5482
                     /locus_tag="VFX21_05450"
     CDS             4544..5482
                     /locus_tag="VFX21_05450"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5095435.1"
                     /translation="MNIAVVGTDHGHIIEIVFRLRDAGGTIVAIIPTEDKIGPWLASQ
                     FPDARGDDPYGDDVDVVATAAIPSERAGIATVAMRAGKDVVSDKPAATSDAQLDALRT
                     CCAETGRGYWVIFSERMSDPAMLTAQRLVGEGRLGTVLHTIGLGPHTLNLPQRPEWFY
                     DPARYGGILVDIGSHQVDQFLTFTGATEARVAAATVRAHDEHPGLQVLGEMLLRADDG
                     RTGYTRVDYFTPAGLGAWGDVRFTVVGTDGFLEVVHMTHSVTVVDGDGRQTIDCQGGS
                     VHWAKDVVEGRAPIAQDHVFAVTDICLRAQAAATEA"
BASE COUNT          930 a         1958 c         1796 g          849 t
ORIGIN      
        1 tgagcgcctg ccactcgtac aaccgccgca cgagcgactc ggccgggaac aagtcgtagg
       61 ccaccgcctg cacgcggtag ttgccgagcc actgccgcgg atgatccgcg ggaaacgact
      121 cgtcgggaaa cccgagatac accgtgccga gcccgccgct cggatgcgcc aacggcgcga
      181 cggcgcgcgc atcttcgatg aacgcgtcga gcgcctccgg ccggatgaag ttcgggatct
      241 cgcacgcgcc ggtgtcgcgc agctgcttgc ggcccgcgtc gacgatgccc cagtcgaggt
      301 cgaccaagtc gtcgaggtcg ggaagcaccg tcatcgcatc ttggagaacg cgttgctctc
      361 actgtcgggc agctggtcga tcggctgcgc gatctcctcc ggtgacggcc cgaactcgtc
      421 cgccagcttc tgcagcgcgg ccatgtcgaa gttgtagagc tgcgccgcgg tctcaccgag
      481 catcatgcgc acctcgttct cgggaacgtc gtggaacgtg ttgcgcagtg actgcttcga
      541 gtacgggtac gtgccttcgt cgtgcgggta gtcgctcgcc cacatgatct tctcgaggcc
      601 gacctggtgg cggcgcttgc actcgagcgg acccatgaag ctcgcggtca cccacacgtt
      661 gcgtgtgaag tactcgctcg ggagcatcgg tagccgctgg tcttcggtga atccgagctc
      721 gccgaccttg ccgccaaaga tccgctcggc gatcgcgtcg gtgcgcggca actcgtcggc
      781 gagccagccc atgcccgact ccgcgaggac gaacttcagc gtcgggtgcc gttcgaacac
      841 accgccgaag atcaacgccc agaacgcgcg gtgcgcgtag aagcccgtct ccaacaacca
      901 gagcacgccg cccgcggggt acttgccgta gtcgggtgcc gcgctccccg agtggtggtt
      961 gaccggcaga ccggtctctt cgagcgcgtc ccagatcggt tcgtacacgt cgttcgcgta
     1021 caacggcggg aggtgtgagt cgggtggcac acccggcagc aggatcccgc cccgcaaccc
     1081 gttctcggca gcccagtaca ccgtttcgac cgcgtcgtcg acgttgttca acaagatctg
     1141 cgccatgccc gcgcgccgtt cgggcgcggc cgcgacgaag tggtcgcgca cccaacggtt
     1201 gtgcgcctgc aagccggccc aacgccgctc gtactcctcg gcggtctgcg gcgcgcgcgc
     1261 catgaagctc gcgctcggga agaagggcgg caccgtgttc gggaacgcga cctcggcgac
     1321 gatgccgtct tcggcttgtt cgttcagccg cgcgtcggag tcccagttgc ggtagcgctt
     1381 cgagccgctc aggtcacgga acgggttctt gtacgcgccg cgccacgcgt cgaactcgtt
     1441 gtgccacttc gtctcgagga actccttgta ctgctcgagc gatccgcccg cgtggcagtc
     1501 ggtcgagatc acgacgtagt tcgaggtcat ggcccgagat ggtagagccg ggcccccgct
     1561 cacgtcgcag ccgtcacgtc cctccgccgg aagctgatgg cagctgccgc aattgcaatc
     1621 cccgcgtacg tcaccagcgc cgccatcgag gtcaacgggc cccgatggaa ttcgacgtcg
     1681 tcaatctgtg cccagcccac gaaggtgacg acgttctcgg tgacgaggta cctcgccaga
     1741 tgaggcttgt agccacggat gagcccttcg acgagaagcg cccagactgc gagcgcgacc
     1801 aacgacgcag cggtgttgcg ggcgatcgtc gcaatgctca acgcgagcat cgcgatcagc
     1861 gcggacacga acgacacgcg caccatcgca gcgcccaacg acgaccacca tgcgccgtcg
     1921 gtcccgtcgg tgctgccgtt cgcccagacc gccgggagcg cggcggacaa gtacacgatc
     1981 tgcaatccga agctgatgag gaacgcgagg atcccggccg agatcgtgcg cgcgaagtgc
     2041 agccgcagcc gcgcgggcac ccacgtcaac acggtcgtga tcgtgcccgc tctccattcg
     2101 gcgccgccga ccgacgcgcc gcagatcgcg gcgccgatcg cgaggaacga cgcggccacg
     2161 gtgaggaagc cctgcccgtg gtcgtaccac cagtagtaca tgttcgcggg atggtcgggc
     2221 gactgcagca gctcgatcgc gttgtcactc gtgttgaaca cgatgacacc gacgaacgca
     2281 cacatgccga gcgcgagcgc gaccatccac cgcacgaggc gccggtgcag cgcccgccgc
     2341 atctcgacga aggtgaccgt gagcaagttc atgcggcgcc gtcttcgcgg tgcgacgtca
     2401 tctgcaggaa cagctcttcg agcgacaccg tctcgggccg cagctcggtg acgtagagac
     2461 cggcgtcggc cagtgtcttg gtgacgagcg ccgcatcggc gggtgccacc gccacccgca
     2521 acagatgctc cgacggttcg cacgcgatcc cggccgcccc gagcgcccgc gcgcccgccg
     2581 cgagatcttc cagcccgacg aggagcgacg ggcgcgccgc cgacgcgagc acgtcctcga
     2641 cgcgtccgtc gagcaggaga cgaccccggt cgatgatcgc gacccggtca cacgtctgtt
     2701 cgacttcggc gagcaggtga ctcgacacga acacggtgcg gccctccgcc gcgagcccgc
     2761 gcaacatctc gcggatctcg cggatgccgg ccgggtcgag accgttggcg ggctcgtcga
     2821 gcacgagcag cgcggggtcg tgcagcaacg cggccgcgag cccgaggcgt tgccgcatgc
     2881 ccaacgagta cttgcgcacc ttgtcgtcac cgcgttcggc caagccgacc tgcgccagca
     2941 cgtcctcgac acgcgcgcgg ccgatgccgt gcagcgcgcc gagcaaccgg aggttctcgc
     3001 gcccgctcat cgtcgggaac agcgcgggcg tctcgacgat cgcgccgatg cgggtgacca
     3061 cccgttcgag atgcgcgggc gtcgattcac cgaagagacg catgcggccc gccgacggcc
     3121 gcacgagacc gagcaggcag cggatcgtcg tcgtcttccc cgagccgttc ggccccaaga
     3181 acccgaacac gccgccttcg gggacttcga ggtgcaggcc gtcgaccgca accgtcgtcc
     3241 cgccgcggaa ccggcggtac tccttgcgca gcccctcgat ctcgatcacc ggcatcccgc
     3301 agctcctgtt cgtcctcact gacgaacggc gacctcgatg tgtaacaccg tagaattgga
     3361 acgtgactga cctcggctac caggccttcg acgccgataa ccactactac gaggcgctcg
     3421 acgcgttcac ccgccacctc gacccgaagc tcggcccacg ctgcgtgcag tggtgcgaga
     3481 tcgacggccg caagtaccac gtggtcggcg gccgggtgag caaggcggtc gtcaacccga
     3541 ccttcaatcc catcgcgctg ccgggcgcga tgcacgacta cttccgcggc aaccccgaca
     3601 agaagcagcc gtgggagttc ctcgccgcgc gcgaaccgat tccggccgaa taccgcgatc
     3661 gtgacgcgcg gatcgcgaag ctcgacgagt tcggcctcga atccgtgtgg ctgttcccga
     3721 cgctcggcat gctctacgag gagctgctca aggacgacgg cgaagcggtg accctcacgt
     3781 tcaccgcgtt caaccgttgg ctcgaggacg actggggctg caactaccgc gaccgcatct
     3841 tcgccgcgcc ctacatctcg ctgtgcgaca tcgactgggc gattcgcgag ctcgagtggg
     3901 cgctcgacca cgacgcccgc acggtcgtca tgcggcccgc ggcgccgcac acccgtgacg
     3961 ggcagatccc cgtgagcgat ccgtgcttcg acccgttctg ggcgcgcgtc gacgaggccg
     4021 gcatcacagt cgtcgcgcac gcgggcgaca gcggctacac gagcaacggc tacgcgcgcg
     4081 acggcttctc cgccgcgttc tccggcggac ggtgggcgcc gagtatcaag agcttccaca
     4141 tcgagcaggc cgcgttcgac ttcctcatca cgctcgtgtt cgacaagctg ttcgagcgct
     4201 tcccgaacgt gcgcatcgcg tccgtcgaga acggctccga cttcctgcgc gacctgttca
     4261 agaagctcgc ctccaccgat cgcaagatgc cgggctactt cggcaccgat cccagcgacg
     4321 cgttccgccg gaacatctgg gtcaacccgt tctgggagga cgacgtgcac gaggtggtcg
     4381 acctcatgag cgccgaccgc gtcatcttcg gctccgactg gccgcacatc gaagggatgc
     4441 cgcggccgct cgactacgtc gccgagctca aggagttcga cgacgctacg cagcagctga
     4501 tcttgcgtga caacgtgcga gcactgaacc aacgcacacc cgcgtgaaca tcgcagtcgt
     4561 cgggaccgat cacggtcaca tcatcgagat cgtgttccgc ctgcgcgacg cgggcggcac
     4621 gatcgtcgcg atcatcccga ccgaggacaa gatcggcccg tggctcgcat cgcagttccc
     4681 cgacgcgcgc ggcgacgatc cgtacggtga cgatgtcgac gtcgtcgcga ccgccgcgat
     4741 accgagcgaa cgagcgggca tcgccacagt tgcgatgcgc gcgggcaagg acgtcgtctc
     4801 cgacaagccc gcggcaacca gcgacgcgca gctcgacgcg ttgcgtacgt gctgcgcgga
     4861 gaccgggcgc ggctactggg tgatcttctc cgagcgcatg agcgatcccg cgatgctcac
     4921 cgcgcagcgg ctcgtgggcg aaggtcggct cgggacggtg ctccacacca tcggcctcgg
     4981 tccgcacacc ctcaatctcc cgcagcggcc ggagtggttc tacgacccgg cccgctacgg
     5041 cggcatcctc gtcgacatcg gctcccacca agtcgaccag ttcctcacct tcaccggggc
     5101 caccgaagcc cgggtggccg cggcgacggt gcgcgcgcac gacgaacatc cgggcctcca
     5161 agtccttgga gagatgctcc tgcgcgccga tgatggacgg acgggctaca cgcgcgtgga
     5221 ctacttcacc cccgccggtc tcggcgcgtg gggcgatgtg cgcttcaccg tggtgggcac
     5281 cgacggcttt ctcgaggtgg tacacatgac gcactcggtg accgtcgtcg acggtgacgg
     5341 gcgccagacg atcgattgcc aaggcgggtc ggtgcattgg gcgaaggacg tcgtcgaggg
     5401 ccgcgccccg atcgcgcagg accacgtgtt cgcggtgacc gacatctgcc tccgcgcgca
     5461 ggcggccgcg acagaagcat gaagcgcacc ctcgcagtcg tcgtcgcgac gttcgtcgcg
     5521 ctactggtcg cgg
//
LOCUS       DASUGA010000277         2773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1701432, whole genome shotgun sequence.
ACCESSION   DASUGA010000277 DASUGA010000000
VERSION     DASUGA010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701432"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VFX21_05455"
     CDS             <1..230
                     /locus_tag="VFX21_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX5095436.1"
                     /translation="CQVPLDDAVVPSLTPVYPGANFPERETYDLYGITFDGHPELTRI
                     LMPDDWTGYPLRKDDPPSRVPVTFKGDPAPR"
     gene            227..1528
                     /gene="nuoD"
                     /locus_tag="VFX21_05460"
     CDS             227..1528
                     /gene="nuoD"
                     /locus_tag="VFX21_05460"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEX5095437.1"
                     /translation="MSTTHDHDHDLQLERQTDEGAQELQARTRTHEMVFTGGVWPEAD
                     ELGDTMIINMGPQHPSTHGVLRLMMELDSEEVLRIKPVIGYLHTGMEKTGEELTYMQG
                     ATNVTRMDYAANFSNELVFSLATERLLDIGVPERAHWIRLLMCELNRMASHLLFQATN
                     GMDIGAVSMMLYGWREREEVLRLFEFVTGLRMNCNYIRPGGVVADLPAGWQDEIRRVC
                     DLVEKGVAEYDALLTGNPIWQERTVGVGIITTEQCLERSITGPILRSTGFAWDLRKSQ
                     PLLPYDEVDFDVIYTSNGDVFDRYRIRLYEILESVKLVRQCIDKMPGGDFRVQDKKVT
                     PPPRARIDESMEALIHHFKLFTEGFKVPAGETYVAIESPRGEIGCYMVSDGSAKPVRM
                     HIRGPSFYNLQAIDPMAGGSLVADAVAIVSSIDPIMGEVDR"
     gene            1532..2020
                     /locus_tag="VFX21_05465"
     CDS             1532..2020
                     /locus_tag="VFX21_05465"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX5095438.1"
                     /translation="MAFSDQNLQRARAIVAQYPRPKSAILPLAHLAQDQDGWLSPEAM
                     DEIATLTGETTAIVQGTCSFYTMFKRRPCGRLVVSVCTNVTCLVTGGPEILDHLDTKY
                     SMDAEVTVEEVECIAACGGAPSMQVNYEFHEKLTPESAEAIVEQYKSGSLTARTISGS
                     HV"
     gene            2023..>2773
                     /locus_tag="VFX21_05470"
     CDS             2023..>2773
                     /locus_tag="VFX21_05470"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018234668.1"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HEX5095439.1"
                     /translation="MEERRIVSQRLHERPHDSWTIAGALAGGAYEVLREVLRQGNPDD
                     VQEQVKLSGLRGRGGANFSTGQKWSFLPAGSYPRYLVVNGDEGEPSTFKDRLLVEQDP
                     HQLVEGIVISAYAIGCNLAFVYLRGEFALGYERLTQAINDARAQGFVGPNVCGSGFDV
                     EIVVHRGAGAYICGEETALLESLEGERGMPRLKPPFPAIEGLYAKPTVVNNVETLSTV
                     PHIMKMGGAEYAKLGVNKSTGTRLVALSGHVK"
BASE COUNT          508 a          910 c          922 g          433 t
ORIGIN      
        1 tctgccaagt gccgctcgac gatgcggtgg tgccgtcgct gacgcccgtg tatcccggcg
       61 cgaactttcc ggagcgcgag acgtacgacc tctacggcat caccttcgac ggtcatcccg
      121 agttgacgcg catcctcatg cccgacgact ggaccggata tcccttgcgc aaggacgatc
      181 cgccgagccg cgtgccggtg acgttcaagg gagacccggc gccgcgatga gcacgacgca
      241 cgaccacgat cacgatctgc agctcgagcg gcagaccgac gagggcgcgc aggagctcca
      301 agcacgcacg cgcacgcacg agatggtgtt caccggcggc gtgtggcccg aagccgacga
      361 gctcggcgac acgatgatca tcaacatggg gccgcagcac ccgtcgacgc acggcgtgtt
      421 gcgactcatg atggagctcg actccgaaga ggtgctgcgc atcaagccgg tgatcggtta
      481 cctgcacacc ggcatggaga agaccggcga agagctcacg tacatgcagg gcgcgaccaa
      541 cgtgacccgc atggactacg cggcgaactt ctcgaacgag ctcgtcttca gcctcgcgac
      601 ggagcggctg ctggacatcg gcgtgcccga gcgcgcgcac tggatccgcc tgctcatgtg
      661 cgagctcaac cgcatggcgt cgcatctgtt gttccaggcc acgaacggca tggacatcgg
      721 cgcggtgtcg atgatgctct acggctggcg cgagcgcgag gaggtgctgc ggctcttcga
      781 gttcgtcacc gggctgcgca tgaactgcaa ctacatccgg ccgggtggcg tcgtcgccga
      841 cttgcccgcc gggtggcaag acgagattcg gcgtgtctgc gacctcgtcg agaaaggtgt
      901 tgccgagtac gacgcgctgc tgaccgggaa cccgatctgg caggagcgta cggtcggcgt
      961 cggcatcatc accaccgagc agtgcctcga acgcagcatc accggcccga tcctgcgctc
     1021 gacgggattt gcgtgggacc tgcgcaagtc gcagccgttg ctgccctacg acgaggtcga
     1081 tttcgacgtc atatacacga gcaacggcga cgtcttcgac cggtaccgca tccggctcta
     1141 cgagatcctc gagtcggtga agctcgtgcg ccagtgcatc gacaagatgc cgggcggcga
     1201 cttccgcgtc caggacaaga aggtcacgcc gcctccgcgc gcccgcatcg acgagtcgat
     1261 ggaagcgctg atccaccact tcaagctgtt caccgagggc ttcaaggtgc ccgcgggcga
     1321 gacgtacgtc gcgatcgagt caccgcgcgg tgagatcggc tgctacatgg tgagcgacgg
     1381 ttcggcgaag ccggtgcgga tgcacatccg cggcccgtcg ttctacaacc tgcaggcgat
     1441 tgatccgatg gccgggggca gcctcgtcgc cgatgccgtc gcgatcgtgt cgagcatcga
     1501 cccgatcatg ggcgaagtgg accggtaaga gatggccttc tcggaccaga acctgcagcg
     1561 cgcgcgggcg atcgtcgcgc agtacccgcg gccgaagtcc gcgatcttgc cgctcgcgca
     1621 cctcgcccag gaccaagacg gctggctgtc gcccgaagcg atggacgaga tcgcgacgct
     1681 gacgggtgag acgaccgcga tcgtgcaggg gacgtgctcc ttctacacga tgttcaagcg
     1741 ccgtccgtgc ggccggctgg tcgtgtcggt ctgcacgaac gtgacgtgtc tcgtgacggg
     1801 cgggcccgag atcctcgacc acctcgacac caagtactcg atggatgccg aagtcaccgt
     1861 cgaggaagtc gagtgcatcg cggcctgcgg aggtgcaccg tcgatgcagg tcaactacga
     1921 gttccacgaa aagctcacgc ccgagtcggc ggaagcgatc gtcgagcagt acaagagcgg
     1981 ctcgctcacc gcccgcacca tctccggaag ccacgtatag atatggaaga acgccgcatc
     2041 gtctcgcaac gcctgcacga acggccccac gactcgtgga cgatcgcggg cgcgctcgcg
     2101 ggcggcgcgt acgaagtgct gcgcgaggtg ctgcgccagg gcaaccccga cgacgtgcag
     2161 gagcaggtga agctgtcggg gctgcgcggc cgcggtggcg ccaacttctc gaccggccag
     2221 aagtggtcgt tcctcccggc cgggagctac ccgcgctatc tcgtcgtcaa cggcgacgaa
     2281 ggcgagccgt cgacgttcaa ggaccgcttg ctggtcgaac aagacccgca ccagctcgtc
     2341 gaaggcatcg tcatctctgc ctacgcgatc ggctgcaacc tcgcgttcgt ctacctgcgc
     2401 ggcgagttcg cgctcggcta cgaacggctc acgcaggcga tcaacgacgc acgcgcgcag
     2461 ggattcgtcg gccccaacgt ctgtggatcc ggcttcgacg tcgagatcgt cgtgcaccgc
     2521 ggggcgggtg catacatctg cggcgaggag accgcgctgc tcgagtcgct cgagggcgag
     2581 cgcggcatgc cgcggttgaa gccgcccttc cccgcgatcg aaggactcta cgcgaagccg
     2641 accgtcgtga acaacgtcga gacgctgtcg acggtgccgc acatcatgaa gatgggcggc
     2701 gccgagtacg ccaagctcgg cgtcaacaag tcgacgggca cgcgcctcgt cgcgctgtcg
     2761 gggcacgtca aga
//
LOCUS       DASUGA010000278         2630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_151556, whole genome shotgun sequence.
ACCESSION   DASUGA010000278 DASUGA010000000
VERSION     DASUGA010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151556"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFX21_05475"
     CDS             <1..217
                     /locus_tag="VFX21_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095440.1"
                     /translation="VEPILAAHGFTAAPSTPTSTAAHPIRRIDYIACDEAFGIGATRV
                     HVPLVSDHRPIVADLIVVRRAPDRPSE"
     gene            274..1464
                     /locus_tag="VFX21_05480"
     CDS             274..1464
                     /locus_tag="VFX21_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011645091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5095441.1"
                     /translation="MAYAYRPMHDADSHIMEEPDWLHPYLDAATKSRFPHVWTVDSEP
                     GRWGIDKERRRHAEPEYRAENASQILLRKNFSATGSFINDDRPEALDHLGFASQLVFD
                     TFTSSPILRFDRDGESELAVTLARAQHRAVLDWCSVDPRLLAVTVVPVGDMDAAVALT
                     RDAIDGGTAAIQIGQYCTPGHSPSHIGLEPMWAMCAAAGVPVLLHVAGAGENVMHPAY
                     FENGRPPVPDFHGGDTNFKSIDYMSIPLPVMQTLNAIVIDGILQRHPNLRVGVIELGA
                     SWLPGWMRMLDSAFEAFRKNEDRLQQMEMKPSEYVQRQVRATPYPHEDTGWTIANSGR
                     QVCLFSSDFPHVEGGRNPIGRFERSLDAAEIGGRARDGFYFDNFVDLMRPVLEARGLA
                     TVAA"
     gene            1564..1923
                     /locus_tag="VFX21_05485"
     CDS             1564..1923
                     /locus_tag="VFX21_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095442.1"
                     /translation="MSARSGAHASGDDARYPFELWLTCPDCGDIVVPAKSCDVYLDLS
                     HDRHLVAYRCPRCRRRGTCPLDDEHVAWLMERGHAVRVLTVAAELHEPRPGGPAFQWD
                     DILAFHEMLSDGPMRLP"
     gene            2011..>2630
                     /locus_tag="VFX21_05490"
     CDS             2011..>2630
                     /locus_tag="VFX21_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095443.1"
                     /translation="MGLGRKGREQRDARTAEAIDEIRQELAKLDTTMRSMVADLNTTL
                     EAQMRITIASVERARESGVGALEHALQEFGSREEQLLDVLRQVGRSYELLAEHITADK
                     IERRTLIDALGGAVRAVSGAVPKPRLIGGSILNATEDDVVVETETAEATEIAGTADDE
                     GEEINLTRAEHAVQQATMQAVEVRCRFGDRWVDGFEVADVIDDGTS"
BASE COUNT          434 a          909 c          895 g          392 t
ORIGIN      
        1 cgtcgaaccg atcctcgccg cgcacggctt caccgccgca ccgtcgacac cgacgtcgac
       61 ggctgcgcac ccgatccgcc gcatcgacta catcgcgtgc gacgaggcgt tcggcatcgg
      121 cgcaacgcgc gtgcatgtgc cgctcgtcag cgatcaccgg ccgatcgtcg ccgacttgat
      181 cgtcgtccgc cgtgcgcccg accgaccgtc ggagtagtcg gtctcgaagg atgaggcggg
      241 accgagcgcg ctaccgcggc gtatcgttcg gtcatggcgt acgcataccg acccatgcac
      301 gatgccgact cgcacatcat ggaggagccc gactggctgc atccctacct cgatgccgcg
      361 accaagtcac gtttcccgca cgtgtggacg gtcgactccg aaccgggccg gtggggcatc
      421 gacaaggaac ggcgccggca cgcggaaccg gagtaccgcg ccgagaacgc gtcgcagatc
      481 ctgttgcgca agaacttctc cgcgaccggc tcgttcatca acgacgaccg cccggaagcc
      541 ctcgatcatc tcgggttcgc gagccagctc gtcttcgaca cgttcacgag ctcaccgatc
      601 ttgcggttcg accgcgacgg ggagtcggag ctcgccgtca cgctcgcccg cgcgcaacat
      661 cgcgcggtgc tcgattggtg ctcggttgat ccgcgcctgc tcgcggtcac ggtcgtgccg
      721 gtcggcgaca tggatgccgc ggtcgcgctc acgcgcgacg cgatcgacgg gggcaccgcg
      781 gcgatccaga tcgggcagta ctgcacaccg ggccactcgc cgagtcacat cggcttggag
      841 ccgatgtggg ccatgtgcgc ggcagcgggt gtccccgtgt tgctgcacgt cgccggtgcg
      901 ggcgagaacg tcatgcatcc cgcgtacttc gagaacggca ggccgcccgt acccgacttc
      961 cacggtggcg acacgaactt caagtcgatc gactacatgt cgatcccgct gccggtgatg
     1021 cagacgctga acgcgatcgt catcgacggc atcctgcaac ggcatcccaa tctgcgcgtc
     1081 ggtgtcatcg agctcggtgc atcgtggctg ccgggctgga tgcgcatgct cgactccgcg
     1141 ttcgaagcgt tccgcaagaa cgaagaccgg ctccagcaga tggagatgaa gccgagcgag
     1201 tacgtgcagc ggcaagtgcg cgcgacgccg tacccgcacg aggacaccgg atggacgatc
     1261 gcgaacagcg gccggcaggt gtgcctgttc tcctccgact tcccgcacgt cgaaggcggc
     1321 cggaatccga tcggacgctt cgagcgcagc ctcgacgcgg ccgagatcgg ggggcgggcg
     1381 cgcgacggct tctacttcga caacttcgtc gacctgatgc ggcccgtgct cgaggcccga
     1441 ggcctggcga cggtcgcggc ctgagccgat tcgagccgag ccgagcgcga atgctggcgc
     1501 gcccggggcg cctgcgcgaa acttcgagac cgggcggggc aggggccggc ggggagtgtt
     1561 gccatgtctg cccgatccgg cgctcacgcg tcgggcgacg acgcccggta tccgttcgag
     1621 ctgtggctca cgtgtcccga ctgcggcgac atcgtcgtcc ccgccaagag ttgcgacgtg
     1681 tatctcgatc tctcgcacga ccggcacctg gttgcctacc gctgcccgcg gtgccgccgg
     1741 cgcgggacct gcccgctcga cgacgagcac gtcgcctggc tgatggagcg cgggcacgcg
     1801 gtccgcgtcc tgaccgtcgc ggcggaattg cacgagccgc gccccggcgg cccggccttc
     1861 caatgggacg acatcctcgc cttccacgag atgctctcgg acgggcccat gcgcttgccc
     1921 tgaccggagc cccggttgcg cgcgatccgc gcgatcggga gcaactcggc ttacgctgcg
     1981 tccgacggca ggacaatgcg ggggttcccg atggggctcg gccgaaaggg acgagaacag
     2041 cgcgacgcgc gcacggccga ggcgatcgac gagatccgcc aagagctggc gaagctcgac
     2101 acgaccatgc ggtcgatggt cgcggatctc aacaccacac tcgaagcgca gatgcgcatc
     2161 acgatcgcga gcgtggagcg cgcccgcgag tcgggtgtcg gcgcgctcga gcacgcgctg
     2221 caagagttcg gctcccgcga ggagcagctc ctcgacgtct tgcgccaagt cggccgttcc
     2281 tacgagctcc tcgccgagca catcaccgcc gacaagatcg agcggcgcac gctgatcgac
     2341 gcgttgggcg gtgcagtgcg tgccgtgtcg ggcgcagtgc cgaagccgcg actgatcggc
     2401 ggatcgatcc tcaacgcgac cgaggacgat gtcgtcgtcg agaccgagac ggccgaggcc
     2461 accgagatcg ccggcaccgc cgacgacgaa ggcgaagaga tcaacctcac acgcgccgag
     2521 cacgcggtgc agcaggcgac gatgcaagcc gtcgaggtgc gctgtcgttt cggcgaccgg
     2581 tgggtcgacg gcttcgaagt cgccgacgtg atcgacgacg gcacatcgtt
//
LOCUS       DASUGA010000279         2541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_807480, whole genome shotgun sequence.
ACCESSION   DASUGA010000279 DASUGA010000000
VERSION     DASUGA010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807480"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1014)
                     /locus_tag="VFX21_05495"
     CDS             complement(37..1014)
                     /locus_tag="VFX21_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX5095444.1"
                     /translation="MPDVSRLLRAIDRFQQRHRPLAFGFGVIKKFGDDQGGNLAALMT
                     YYGFLSLFPLLLVMVTVLSYVLDDNPDLQRDILNSALADFPVLGDQIRNNIGSLRGNG
                     IGLIIGILVTFYGGLGIANSVQDAMNRVWSVPHHSRPGFFPRIARSLALIGVLGLGIL
                     FTTFLATVAAASGNVNGEVRVMTAFAAFVFNIGLFALAFKALTAKKLAWTDVLPGALI
                     AAFGYGVLQVIGGALLTRQLKGMSETYGVFAAVIGLLLWIFLLARVTIYAAEVNAVRT
                     HKLWPRSFNPPPLTDGDQRAFEMYARVEGRRKGERIHVELPEDEIQGTS"
     gene            1182..2363
                     /locus_tag="VFX21_05500"
     CDS             1182..2363
                     /locus_tag="VFX21_05500"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5095445.1"
                     /translation="MTTFKDLGLKAEIVDALAAQGIDEPFAIQAATIEDALAGRDVCG
                     KAKTGSGKTLAFGLPTLQAVEPATPGHPKALVLVPTRELAKQVVDVLSPIGKEIGVRV
                     AAFYGGTSMDRQIRNLRRRIEVAVATPGRLIDLLERNELDLDQISILVVDEADRMADM
                     GFLPQVEWLLRKVPDDRQTLLFSATLDREVDVLVRRYMVDPVKHEVVSETQTVDEMLH
                     RFLKVHELDRVKVLASIAGGVDRTIVFCRTKRGADKLVSQLKREGVNAAAIHGDLRQS
                     AREKALKEFQGDGNHGVLVATDVAARGIHVDDIEVVVHYDPPEEHKSYVHRSGRTARA
                     GNEGIVVTFVLWDQELEVERLQRRLGMREPIIEVFSNDPRLADLAHLDGPAPKRRAVS
                     A"
BASE COUNT          477 a          841 c          819 g          404 t
ORIGIN      
        1 agggcgggaa attttcccgg cctgaagcgc ctcttgctag ctcgtgccct ggatttcgtc
       61 ttcgggaagc tcgacgtgga tgcgctcccc tttgcgccgc ccttcgaccc gtgcgtacat
      121 ctcgaacgca cgctgatcgc cgtcggtcaa gggcggcggg ttgaagctcc gaggccacaa
      181 cttgtgggtg cgcactgcgt tgacctcggc cgcgtagatg gtgacgcgag ccaagaggaa
      241 gatccacagg agcaggccga tcacggccgc gaacacgccg taggtctcgc tcatgccctt
      301 caactgccgc gtgagcaacg caccaccgat cacctgcaac acgccgtagc cgaacgcggc
      361 aatcagcgcc cccggcaaca cgtcggtcca cgcgagcttc ttcgcggtca gcgctttgaa
      421 cgcgagcgcg aacagcccga tgttgaacac gaaggcagcg aacgcggtca tgacgcgaac
      481 ctcgccgttc acgtttcctg acgcagccgc gacagtcgcg aggaacgtcg tgaacaagat
      541 gccgaggccg agcaccccga tcagcgccag gctgcgcgcg atgcgcggaa agaagccggg
      601 acgcgagtga tgcggaaccg accacactcg gttcatcgcg tcttgcaccg agttcgcgat
      661 gccgagaccg ccgtagaacg tcacgagaat gccgatgatc aagccgatgc cgttgccgcg
      721 caacgacccg atgttgttgc ggatctgatc gccgagcacc gggaagtcgg ccagcgccga
      781 gttcaagatg tcgcgttgga ggtcgggatt gtcgtcgagg acgtagctca gcaccgtcac
      841 catgacgagc aacagcggga agagcgagag gaagccgtag taggtcatca gcgcggcgag
      901 attgccgccc tggtcgtcgc cgaacttctt gatgacgccg aatccgaaag ccaagggccg
      961 gtggcgttgc tggaatcggt cgattgcacg cagcagccgg ctcacgtcgg gcatcaaagc
     1021 cgctgtaccc cgatgaccgg agtatccctc ttgaccaggt agtatggcgg agccgggtgc
     1081 ccccaacttc gggacccgcg gccgtccctc acctgtttcg ctgcaacgga gctgccgagg
     1141 atcggcctcc gaccgatgta acaggtatca acaggagaga cgtgaccacg ttcaaagacc
     1201 tcggcctcaa ggccgagatc gtcgacgccc ttgccgcgca ggggatcgac gaacccttcg
     1261 ccattcaggc cgccacgatc gaagacgccc tcgccggccg cgatgtgtgc ggcaaagcca
     1321 agaccggatc gggcaagacg ctcgcgttcg ggcttcccac cttgcaggcc gtcgagcccg
     1381 cgacgccggg acaccccaaa gcactcgtgc tcgtgccgac ccgcgaactc gcaaagcagg
     1441 tcgtcgacgt cctgagtccg atcggcaagg agatcggcgt gcgcgtcgcc gccttctacg
     1501 gcggcacgtc gatggaccgc cagattcgca acctgcgccg ccgcatcgag gtcgcggtcg
     1561 cgacgccggg ccgcttgatc gacctgctcg agcgcaacga actcgacctc gaccagatct
     1621 cgatcctcgt cgtcgacgaa gccgaccgca tggccgacat gggcttcttg ccccaggtcg
     1681 agtggctgtt gcgcaaagtg cccgacgacc gccagacgtt gttgttctcc gccacgctcg
     1741 accgcgaggt cgacgtgctc gtgcggcgct acatggtcga ccccgtgaag cacgaggtcg
     1801 tttccgaaac gcaaacggtc gacgagatgc tccaccgctt cctgaaggtc cacgagctcg
     1861 accgcgtcaa ggtgctcgcg tcgatcgcag gcggcgttga ccgcacgatc gtgttctgcc
     1921 gtaccaagcg cggcgccgac aagctcgtgt cgcagctgaa gcgcgaaggc gtgaacgcgg
     1981 cggcgatcca cggtgacctc cggcagtcag cacgtgagaa ggcgttgaag gagtttcaag
     2041 gcgacggcaa ccacggtgtg ctcgttgcaa ccgacgtggc cgctcgcggt atccacgtcg
     2101 acgatatcga ggtggtcgtc cattacgacc cgccggaaga gcacaagtcg tatgtccacc
     2161 gttcgggccg caccgcgcgg gcgggcaacg agggcatcgt ggtgacgttc gtgttgtggg
     2221 accaggaact cgaggtcgag cggctccagc ggcggctcgg tatgcgggaa ccgatcatcg
     2281 aggtcttctc gaacgatccg cggctggccg acctcgccca cctcgacggg cccgcgccga
     2341 aacgccgggc tgtttcggcc tagcgctggg tacgattcac ggctgtgact cagacccccg
     2401 atcccggcgg acccgtgacg cccgaagtcg tcgacctgcc gatcccggcc ggtgacgcca
     2461 gcggcgaagg cgagcaggtc tcccagccga cgaagctgat ccgtattgcg tcgatggtgc
     2521 gcgcgctgct cgacgaggcc c
//
LOCUS       DASUGA010000280         4514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_576623, whole genome shotgun sequence.
ACCESSION   DASUGA010000280 DASUGA010000000
VERSION     DASUGA010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576623"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VFX21_05505"
     CDS             <1..187
                     /locus_tag="VFX21_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095446.1"
                     /translation="ANLANAVNTETPPGQMAPITTGPTVPAGADATTVPGDTATTAPA
                     ATDTTPTTGAPTATSTP"
     gene            184..1215
                     /locus_tag="VFX21_05510"
     CDS             184..1215
                     /locus_tag="VFX21_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEX5095447.1"
                     /translation="MKVVVLVGGEGTRLRPLTYTTPKPLLPIANQAHLERQLAWLAAH
                     GVDEAVLSMGYLPDAFHNHFAHDAQGNDVFGDMRLRYAVEDEPLGTAGAIRFAAEGID
                     ERFVVCNGDVLTDTDLGAMVEFHDSCGAEATISLTQVDDPSAFGVVPTRDDGAVIAFV
                     EKPPPGKAPSNWINAGTYVLEPSFLERIPERLNVSVERETFPRMLEQPGLLYGYKTDA
                     YWLDIGTPEKYLQAQLDVMDGRTGGSPVAGRVLLGDGAHVDEGAHVSDSVIGARAVVE
                     AGARVEHSVLHAGVRVSHGSTVRDSIVGADSVLKPEVALSALTIIGAGMTVPSGSTIS
                     GGRLPSDRA"
     gene            1212..2162
                     /locus_tag="VFX21_05515"
     CDS             1212..2162
                     /locus_tag="VFX21_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5095448.1"
                     /translation="MRALVTGGAGFIGSTLVDRLLAEGWAVDVVDDLSSGSLGNLGDA
                     RSQPGRKFSFHRLDVASPAVTELIVHRRPDIVFHLAAQIDVRVSVAKPAQDAMVNIIG
                     SLNVCEGALTAGVKKVVFAASGGTLYGTPEVLPVREGHPQRPESPYGVAKKAVGDYLH
                     YYREVRGLEYTALALANVYGPRQDAHGEAGVVSIFAGKLLSHERPIIFGDGAQTRDFV
                     YVDDVADAFVRATDKGGGLVLNIGTGVETSVQQLFDVMARLTGFKETARYDPPRVGEL
                     QRSALDPSRAAIHLGWKPWTALDEGVARTLEHFKGARSAK"
     gene            complement(2178..2786)
                     /locus_tag="VFX21_05520"
     CDS             complement(2178..2786)
                     /locus_tag="VFX21_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX5095449.1"
                     /translation="MTRVPAFLEARRSIRAFVDEAVPRETLDALVEAACIAPAPHHSR
                     PWRFVVVDTEDAKAALANGMGARWRTDLTGDGLDPARIDELVDASHRKITGAPALVLG
                     CLTWDGLDRYPDERRRRAEWGMALLSLGAAVENLMVAAADRALASCWVAAPIFCPEEA
                     RDALALPGEWLPHAMVMVGVPDPAYVGRLRPPIPLEELREFR"
     gene            complement(2783..3490)
                     /gene="cofE"
                     /locus_tag="VFX21_05525"
     CDS             complement(2783..3490)
                     /gene="cofE"
                     /locus_tag="VFX21_05525"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX5095450.1"
                     /translation="MTLTLVPIRGIGEIKPGDEIAERVAAAARAQDTPLADGDCLVVT
                     QKIVSKAEGRLVELDPDDLAARRALVESESVRIVRRRGDLIISETTHGFVCANAGIDL
                     SNVEAGHAALLPVDPDRSAKHIRDALRARAGVDVAVVVSDTFGRAWRRGLTDVAIGVS
                     GISAIVDLRETPDALGRILHVTEVAIADEIAGAAELVMGKASGVPVAIVRGLDRAWFG
                     DGSYRDLVRPPAEDLFR"
     gene            complement(3487..4422)
                     /gene="cofD"
                     /locus_tag="VFX21_05530"
     CDS             complement(3487..4422)
                     /gene="cofD"
                     /locus_tag="VFX21_05530"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEX5095451.1"
                     /translation="MLVALAGGVGAARFLAGLIRAVPPESVTVIVNTADDETFHGLHV
                     SPDLDSVTYTLAGASNVAQGWGLEGESFAALEALKRYPVETWFGLGDKDLATHLFRTE
                     RLRTGAPLSAVTGEIARAWGLTTRLLPMSDDDVRTRIEVRDGTTTRELRMQEWFVRDR
                     AEPEVVAVRFEGAEAARPAPGVLDALRTAETILLCPSNPVISIGPILAVPGIRDVLVE
                     RRDRVVGVSPIVGGAPVKGPADRLMGPLGIEVSCVGVAREYAPFCHTLVIDAVDEART
                     SEVEATGVRAVVADTMMRSPEIAAALAERALAAVA"
BASE COUNT          699 a         1605 c         1604 g          606 t
ORIGIN      
        1 cgccaacctg gcgaacgcgg tcaacaccga gacgccgccg gggcagatgg cgccgatcac
       61 caccggtccg acggtaccgg ccggcgcgga cgcgacgacc gtgcccggtg acaccgcgac
      121 gacggcaccg gccgccaccg atacgacacc gaccacgggc gcgccgacgg caaccagcac
      181 tccatgaagg tcgtcgtcct ggttgggggt gaagggacac ggctgcggcc gctgacgtac
      241 acgacgccga agccgctgct cccgatcgcg aaccaggcgc acctcgaacg acagctcgcg
      301 tggttggcgg cgcacggcgt cgacgaggcg gtgctgtcga tggggtatct gccggacgcg
      361 ttccacaatc acttcgcgca cgacgcgcag ggcaacgacg tcttcggcga catgcgcctg
      421 cgctatgccg tcgaagacga gccgctcggc acggccggcg cgatccgctt cgcggcggag
      481 ggcatcgacg agcgcttcgt cgtgtgcaac ggcgacgtcc tcaccgacac cgatctcggt
      541 gcgatggtcg agttccacga ctcctgcggt gcggaggcga cgatctcgct gacgcaggtc
      601 gacgacccga gcgcgttcgg ggtggtgccg acccgcgacg acggcgcggt gatcgcgttc
      661 gtcgagaagc cgccgccggg caaggcgccg agcaactgga tcaacgccgg cacctacgta
      721 ctggagccct cgttcctgga gcgcattccc gagcgcttga acgtctcggt ggagcgcgag
      781 acgttcccgc gcatgctcga gcaaccgggg ttgttgtacg gctacaagac cgacgcctac
      841 tggctcgaca tcggcacgcc cgagaagtac ttgcaggccc agctcgacgt gatggacggc
      901 cgcaccggcg ggtcaccggt cgccggccgc gttctactcg gtgacggtgc gcacgtcgac
      961 gagggcgcgc acgtctcgga ctccgtcatc ggtgcgcgcg cggtggtcga agcgggcgcc
     1021 cgtgtcgagc actccgtgtt gcatgccggc gtacgcgtgt cgcacggcag cacggtccgc
     1081 gactccatcg tcggggccga ctcggtgctc aagcccgaag tcgcgctgag cgcgctgacg
     1141 atcatcggcg cggggatgac ggtgccgtcg ggcagcacca tctccggcgg gcggctcccg
     1201 agcgatcgcg catgagggca ctcgtcaccg gcggagccgg cttcatcggg tccacgctcg
     1261 tcgaccggct gctcgccgaa ggctgggcgg tcgacgtcgt cgacgacctg tcgagcggtt
     1321 cgctcggcaa tctcggcgac gcgcgcagcc aacccggccg caagttctcg ttccatcgcc
     1381 tcgacgtggc gtcaccggcg gtcaccgagc tcatcgtgca ccggcggccc gacatcgtgt
     1441 tccacctcgc ggcgcagatc gacgtgcggg tgtcggtcgc gaagccggcg caggacgcga
     1501 tggtcaacat catcggctcc ttgaacgtct gcgaaggcgc gttgaccgcg ggagtgaaga
     1561 aggtcgtgtt cgcggcgtcc ggcggcacgc tctacggcac gcccgaagtg ttgcccgtgc
     1621 gggaaggcca tccgcagcga cccgagtcgc cctacggcgt cgcgaagaag gccgtcggcg
     1681 attacctgca ctactaccgc gaggtacgcg ggctcgagta cacggccctc gcgctcgcca
     1741 acgtctacgg gccgcgccag gacgcgcacg gcgaagccgg ggtcgtctcg atcttcgcgg
     1801 ggaagttgct gtcgcacgag cggccgatca tcttcggtga cggcgcgcag acgcgcgact
     1861 tcgtctatgt cgacgacgtc gccgacgcgt tcgtgcgcgc caccgacaag ggcggcggtc
     1921 tcgtcttgaa catcggcacg ggcgtcgaga cgtcggtgca acagctgttc gacgtcatgg
     1981 cccgcctgac gggcttcaag gagaccgccc gctacgatcc gccgcgcgtc ggcgaactgc
     2041 agcgcagcgc tctcgatccg tcgcgcgcgg cgatccacct cggctggaag ccctggacgg
     2101 cactcgacga aggcgtcgcc cgcaccctcg agcacttcaa gggcgcgcgc agcgccaagt
     2161 agccaacgtt tcgacggcta ccgaaactcg cgcaactctt cgagggggat cggcgggcgg
     2221 agccgaccga cgtacgccgg atcgggcaca ccgaccatca ccatcgcatg cggcagccac
     2281 tcgccgggga gtgcgagcgc gtcgcgcgcc tcctccgggc agaagatcgg cgccgcgacc
     2341 cagcacgacg cgagcgcacg gtcggcggcc gcgaccatca ggttctcgac cgcggcgccc
     2401 agcgagagca acgccatgcc ccactcggcg cgccggcgcc gttcgtcggg atagcggtcg
     2461 aggccgtccc aggtgagaca cccgagcacg agcgcgggcg caccggtgat cttgcggtgc
     2521 gacgcgtcga ccagctcgtc gatgcgagcc gggtcgaggc cgtcaccggt caggtccgtg
     2581 cgccagcgcg cgcccatgcc gttcgcgagc gccgccttcg catcctcggt gtcgacgacg
     2641 acgaatcgcc acggtcgcga gtggtggggc gcgggggcaa tgcacgcggc ctcgaccagc
     2701 gcgtcgaggg tctcgcgcgg cacggcctcg tcgacgaacg cacggatcga acggcgcgct
     2761 tcgaggaacg cggggacgcg cgtcaccgga acaggtcttc ggccggcggc cggacgaggt
     2821 cgcggtagga gccgtcgccg aaccaggcgc ggtcgagccc gcgcacgatc gcgacgggca
     2881 cgccggacgc cttgcccatg acgagctcgg cggcaccggc gatctcgtcc gcgatcgcga
     2941 cttcggtgac gtgcaggatg cggccgagcg cgtcgggtgt ctcgcgcagg tcgacgatcg
     3001 ccgagatgcc gctcacgccg atcgcgacgt cggtgaggcc gcggcgccac gcccgcccga
     3061 acgtgtccga gacgaccacc gccacgtcga cgccggcccg ggcccggagt gcgtcacgga
     3121 tgtgcttcgc cgaccgatcg ggatcgaccg gcagcagcgc ggcgtgcccg gcttcgacat
     3181 tggagaggtc gatgcccgcg ttcgcgcaca cgaagccgtg cgtcgtctcg ctgatgatga
     3241 ggtcgccgcg ccgccgcacg atgcgcacgg actccgactc gaccaacgcc cggcgcgccg
     3301 cgaggtcgtc ggggtcgagc tcaacgaggc gcccctccgc tttcgacacg atcttctgcg
     3361 tcacgacgag gcagtcgccg tcggcgaggg gcgtgtcctg agcgcgcgcg gctgcggcga
     3421 cacgctctgc gatctcgtcg cccggtttga tctcgccgat gccgcggatc ggcacgagcg
     3481 tgagcgtcat gcgacggcgg ccagcgcgcg ctcggcgagc gcggccgcga tctcggggct
     3541 gcgcatcatc gtgtcggcca cgaccgcgcg cacgccggtc gcctccactt cggatgtgcg
     3601 cgcctcgtcg accgcgtcga ttacgagcgt gtggcagaac ggtgcgtact cgcgcgcgac
     3661 gccgacacac gagacctcga tgccgagcgg ccccatcaag cggtcggccg gccccttcac
     3721 cggcgcgccc ccgacgatcg ggctgacgcc gacgacgcgg tcgcgccgct cgacgagcac
     3781 gtcgcggatg ccgggcaccg cgaggatcgg tccgatcgag atcaccgggt tcgaggggca
     3841 cagcaagatc gtctcggccg tccgcagcgc atccagcacg cccggggcgg ggcgggcggc
     3901 ctcggcgccc tcgaagcgca ccgcgaccac ctcgggctcg gcgcggtcac gcacgaacca
     3961 ctcctgcatg cgcaactccc gcgtggtggt gccgtcgcgc acttcgatgc gcgtgcgcac
     4021 gtcgtcgtcc gacatcggca ggaggcgggt cgtgaggccc caggcccggg cgatctcgcc
     4081 cgtcactgcg gagagcggcg cgcccgtgcg cagccgttcg gtgcggaaca ggtgggtcgc
     4141 gaggtccttg tcgccgagtc cgaaccaggt ctcgacggga tagcgcttca gcgcctcgag
     4201 cgcggcgaag ctctcaccct cgagtcccca gccctgggcg acgttggagg cacccgcgag
     4261 cgtgtaggtg accgagtcga ggtcggggga gacgtgcagc ccgtggaacg tctcgtcgtc
     4321 cgcggtgttc acgatcacgg tgacggactc gggcggcacc gcgcgaatca gcccggcgag
     4381 gaaccgggcc gctccgacac cacccgcgag cgcgacgagc acaacgcgaa cctagcgaac
     4441 acgcgaaacg gcggtcctca agaggaccgc cgcttcgcgt caccgcgagc aggtcgaagg
     4501 cgacaaagaa acta
//
LOCUS       DASUGA010000281         3047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_111726, whole genome shotgun sequence.
ACCESSION   DASUGA010000281 DASUGA010000000
VERSION     DASUGA010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111726"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            435..608
                     /locus_tag="VFX21_05535"
     CDS             435..608
                     /locus_tag="VFX21_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095452.1"
                     /translation="MLNGVLLVGLSESRRHSGPPQPPAEGRGKDSSRAKLRVAAPAAA
                     AVAMAGCCAALLV"
     gene            634..945
                     /locus_tag="VFX21_05540"
     CDS             634..945
                     /locus_tag="VFX21_05540"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018655344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX5095453.1"
                     /translation="MSVVKINAITVPKERADELVGRFAARAGEVGQAEGFEAFELLRP
                     SDDRDVFLVYTRWRSQEDFEKWMASAAFGHGHAQHANQGPVSTGAELWSFDVVQHEEA
                     S"
     gene            942..2648
                     /locus_tag="VFX21_05545"
     CDS             942..2648
                     /locus_tag="VFX21_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX5095454.1"
                     /translation="MTDPIIEQLTGEGGPFEIVIEDVLGHPTQVYKSRLTSMRDLMAQ
                     NAARGDLDWVVQGEARYTYAEHDRLARVVAAHLAGLGVERGDRVGLCSANVPEWVITW
                     WACAILGAVVVPLNAWWKSEELEFGLVDSGAKVLIADERRVDAVRDRLPEIRELAHVF
                     SFDDPGAAPVRSFAELTTGDDPGMPPGFVEEDDLLAIMYTSGTTGQPKGATLTHRQAL
                     ANLANIIVSGVAATMRGGTPREGTPDLRSAALLVVPLFHVTGCLSTMMVSYATGGKLV
                     LMPVGRFDPDTAMAMIEREKVTAIGGVPTVMWRILESPNLEKYDLSSVQRASYGGAPA
                     APELVERIEQVFPNLRKTLATAYGLTETASVATAISGDDYFAHPGSVGRAAPTVEVRV
                     VNEDGVDSDPGERGEVWIKGPTVMNRGYWRRPDANEVAFSDGWFHTGDIGYLDAEGYL
                     YLVDRAKDMIIRGGENVYSVEVEHVLFDHPDVVDAAVVGVPHRTLGEEVKAVVQLKPG
                     STATESEIREFCAKHLANFKVPEYVEIRAEPLPRNPAGKVLKNMLRGQETSFAAADDQ
                     AL"
     gene            2655..3023
                     /locus_tag="VFX21_05550"
     CDS             2655..3023
                     /locus_tag="VFX21_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095455.1"
                     /translation="MLFDIHRTWGYVAIVLNAIAGIQLLCAWRWERLRTSWRWWPTII
                     AEAAMMIQVVLGVLLVSVEDYEPPRFHMFYGFVAFITVGLLYSYKYVWKARGWLELAY
                     GLGGLFIMGLGIRAVLQVMA"
BASE COUNT          488 a          984 c         1093 g          482 t
ORIGIN      
        1 tcgaggacgg cctcgcgtcg cggcccgacg tgaaggagat cgtcgatcga ctcgacgcgc
       61 aggccggccc gccccccgac ggcgacgccc tggtcgacga gatcgagcag ttcctccgct
      121 cgcagacgga cgagtaacgc cggactcatc gcgcctcgac aaaggcagcg tcgccgtgct
      181 cgtgccgatc ccggcgccga cgctgcgggc cggtgaccgc gtctacgtgc gggccgatca
      241 gaaggacaag ggcaccgtgt accgcgtgag caccgtgaag gactcgtgga agcacgagtt
      301 cgaggtgctc gacgagcgcg gccaggcgca gacgatcgaa ctgccgggga gcgcgtggcg
      361 cgtcagccgc tcggtgccgt acctcggcgt cccgttctcg ctggctcgtc ggaccgatcc
      421 aggcgaacct gttcgtgctc aacggcgtct tgctggtcgg gctcagcgag tcgcgccgcc
      481 actccggccc gccccaaccg ccggcggagg gccgcggcaa ggactcgtcc cgcgccaagc
      541 tgcgcgtggc cgcgcccgcc gcggccgcgg tcgcgatggc gggttgttgc gctgcattac
      601 tggtctgagc cccggctgcg cgacgatgcg cgcatgtctg tcgtcaagat caatgccatc
      661 acggttccga aagagcgcgc ggacgagctg gtcgggcgat tcgcggcccg cgccggcgag
      721 gtgggccagg ccgaggggtt cgaggcgttc gagctgttgc ggccgagcga cgaccgcgac
      781 gtgttcctcg tgtacacgcg ctggcggtcg caggaagact tcgagaagtg gatggcgagc
      841 gcggcgttcg gtcacgggca cgcgcagcac gccaaccagg gaccggtgag caccggcgcg
      901 gagctctggt cgttcgacgt cgtgcaacac gaagaagcgt cgtgaccgac ccgatcatcg
      961 aacagctgac gggtgaaggc gggccgttcg agatcgtgat cgaagacgtc ctcggtcacc
     1021 cgacgcaggt ctacaagtcg cgcttgacgt cgatgcgcga cttgatggcg cagaacgccg
     1081 cgcgcggtga cctcgactgg gtggtgcagg gcgaggcgcg ctacacgtac gccgagcacg
     1141 atcgactggc gcgcgtcgtc gctgcgcatc tggccgggct cggtgtcgag cgcggcgacc
     1201 gcgtcggctt gtgctccgcg aacgtgcccg agtgggtcat cacctggtgg gcgtgcgcga
     1261 ttctcggcgc cgtcgtcgtg ccgttgaatg cgtggtggaa gagcgaagag ctggagttcg
     1321 ggctcgtcga ctccggcgcc aaggtcctga tcgccgacga acgacgcgtc gacgccgtgc
     1381 gcgaccggct gccggagatc cgcgagctcg cgcacgtgtt cagcttcgac gaccccgggg
     1441 ccgcgccggt tcgatcgttc gccgagttga cgacgggtga cgacccgggg atgccgcccg
     1501 gtttcgtcga ggaagacgac ctcctggcga tcatgtacac gtcgggcacg accgggcagc
     1561 cgaagggtgc gacgctgacg caccgccagg cgctcgcgaa cctcgcgaac atcatcgtga
     1621 gcggcgtcgc ggcgacgatg cgcggcggca caccgcgcga gggcacaccc gacctgcgtt
     1681 ccgccgcgct gctcgtcgtc ccgttgttcc atgtcaccgg ctgcctgtcg acgatgatgg
     1741 tcagctacgc gaccggcggg aagctcgtgc tcatgccggt cggtcgcttc gaccccgaca
     1801 cggcgatggc gatgatcgaa cgcgagaagg tcaccgcgat cggtggcgtg cccacggtga
     1861 tgtggcgcat cctcgaatcg ccgaacctcg agaagtacga cctgtcgtcg gtgcaacgcg
     1921 cgagctacgg cggcgcgccc gcggcaccgg agctggtcga gcgcatcgag caggtgttcc
     1981 cgaacctgcg caagacgctc gcaaccgcgt acggcctcac cgagaccgcg tcagtggcga
     2041 ccgcgatcag cggcgacgac tacttcgcgc accccggttc ggtcggccgc gccgcgccga
     2101 cggtcgaagt gcgcgtcgtg aacgaggacg gcgtcgacag cgatccgggc gagcggggcg
     2161 aggtctggat caagggcccg accgtgatga accgtggcta ttggcgccgg cccgatgcga
     2221 acgaggtcgc gttcagcgac ggctggttcc ataccggcga catcggctat ctcgacgcgg
     2281 agggctacct gtacctggtc gaccgggcca aggacatgat catccgcggc ggcgagaacg
     2341 tctacagcgt cgaggtcgaa cacgtgctgt tcgaccatcc cgacgtcgtc gacgcggccg
     2401 tcgtcggcgt gccacaccgc acgctcgggg aagaagtgaa ggccgtcgtg cagctgaagc
     2461 cgggatcgac ggcgacggag tccgagatcc gcgagttctg tgcgaagcac ctcgcaaact
     2521 tcaaggtgcc cgagtacgtg gagattcgcg ccgaaccgtt gccgcgcaac cccgcgggca
     2581 aggtgctcaa gaacatgttg cgcggccagg aaacgtcgtt cgccgccgcc gacgaccagg
     2641 cgctgtaaca ctcgatgttg ttcgacatcc accggacttg gggctacgtc gcgatcgtgc
     2701 tcaacgcgat cgcgggcatc cagttgttgt gtgcgtggcg gtgggaacgc ctgcggacgt
     2761 cgtggcggtg gtggccgacg atcatcgcgg aagcggcgat gatgatccag gtcgtgctgg
     2821 gcgtgttgct cgtcagcgtc gaggactacg agccaccgcg ctttcacatg ttctacggct
     2881 tcgtcgcgtt catcaccgtc ggcttgctgt actcgtacaa gtacgtgtgg aaggcgcgcg
     2941 gctggttgga gctggcctac ggcctcggcg gcctgttcat catggggttg ggtatacgcg
     3001 ccgtgctaca agtaatggcg tgaacgacaa tcgtcgggtg cgcgtgc
//
LOCUS       DASUGA010000282         3674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1953532, whole genome shotgun sequence.
ACCESSION   DASUGA010000282 DASUGA010000000
VERSION     DASUGA010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1953532"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1117)
                     /locus_tag="VFX21_05555"
     CDS             complement(137..1117)
                     /locus_tag="VFX21_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX5095456.1"
                     /translation="MTDPVVEQVPLGPRWPTIDPFLFCAHHDDAYPRGNDDLGPDASL
                     ADRDIGQDFSGRDGWSMYHGDVVPGFPQHPHRGFETVTLVRQGLIDHSDSLGAKARFG
                     HGDVQWVTAGNGIVHSEMFPLLYRDEPNPIELFQIWLNLPAADKRVDPYFTMLWADDI
                     PTVDGVTVIAGSFNGVDAPAPPPNSWAAHPEADVAIWHVRLEPGASVTLPAAHGPDTV
                     RTLYFFAGDTLRVEDHELGVNTGAVVRCDAPVTLTAGAAAVEALVLQGRPIGEPVAQY
                     GPFVMNSEAEIHEAFADYRRTQFGGWPWPEDGPTHGRDPERFARVGERAL"
     gene            1176..2192
                     /locus_tag="VFX21_05560"
     CDS             1176..2192
                     /locus_tag="VFX21_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5095457.1"
                     /translation="MTLELFTMSIPEPSLVELFSTRAEAEGWDGITFTDSQNLVADPF
                     IGIAIGARVTERLQFMTGVSNPATRHPAALATVAATVQETSGGRMTLGLGRGDTALFH
                     LGRHPQSLNEFFARTDELHTYLNGGVVDIDGYPSQIRWLARAQQPRVPIDIASSGPKV
                     IAYAARTVERITFAVGADPERMAWAVDLARAAMKDAGRTDGDVSFGAYVTVACHPDAA
                     KARALVKGSVAAFAHFSAMPGSTGAGLDERDRELVAEVGRTYDSNMHLVNSADHAQVL
                     PDEFIDRFAVIGDASACAKRLHELVSLGLERLVITGPSFGADREDANTHRTLVCEELL
                     PALR"
     gene            complement(2189..2620)
                     /locus_tag="VFX21_05565"
     CDS             complement(2189..2620)
                     /locus_tag="VFX21_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HEX5095458.1"
                     /translation="MADLDLVRRLGGASHGLTIVATTRPDGSVHATLVNAGVLDHPVN
                     GAPVVGAVVRGSAKKLANIRRSGRASFTFRDGWEWVSVEGPAQIIGPDDPVAGVDVPE
                     LLRAVFKAAGGTHDDWDEYDRVMAAEGRVAVLVTPARVMTN"
     gene            2646..2930
                     /locus_tag="VFX21_05570"
     CDS             2646..2930
                     /locus_tag="VFX21_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX5095459.1"
                     /translation="MALTLCVLLDAVPGNEQQLIDYEDQVLALIPAHGGRVVSRVRAL
                     ESGGPYEVHVIEFPSDDALDAFMSDPQRTALAPQRDRAIAQTTVLRVDQV"
     gene            complement(2921..3373)
                     /locus_tag="VFX21_05575"
     CDS             complement(2921..3373)
                     /locus_tag="VFX21_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095460.1"
                     /translation="MPLRFNHMELTFPRGTLTKEFRREVDSVFVDILGWRATDADVVG
                     QHTHILLPDDGQFILLAEGKKWMESPGYDHLGFLLDTRAEVDDVLEKLEKFAADDDRM
                     QIKRYEDLAAPNLTVHAFYFKFLLPIWFDVQCLEYGAAGGPAKQWRYT"
     gene            3441..>3674
                     /locus_tag="VFX21_05580"
     CDS             3441..>3674
                     /locus_tag="VFX21_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012359471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEX5095461.1"
                     /translation="MDTLQMVEPPALSRESERVRHLSAMKRRATGLLLVMAIAFVVVT
                     LVTDEEGVAGYVQAALEASLVGGLADWFAVTALF"
BASE COUNT          577 a         1298 c         1228 g          571 t
ORIGIN      
        1 ctcgcggtcg gaccgttgtc ttcgttgtcg ccccacaaac ggttcgcggc ctccggcgca
       61 accaagaacg acacaccgga cgcgaagcgg atcgcacccg cggtcaacgc gagtgagtcg
      121 acgcggctct cggcgctcac agtgcccgct cgccgacgcg cgcgaaccgt tcggggtcgc
      181 ggccgtgcgt cggtccgtcc tcgggccacg gccagccgcc gaattgcgtg cgccggtagt
      241 cggcgaacgc ctcgtggatc tccgcctcgc tgttcatgac gaagggtccg tactgcgcga
      301 cgggttcgcc gatcgggcgg ccctgcaaga cgagcgcttc gaccgcggcc gcgcccgcgg
      361 tcaatgtcac cggcgcgtcg cagcgcacga ccgcacccgt gttcacgccg agctcgtgat
      421 cttcgacgcg caaggtgtcg ccggcgaaga agtacagggt gcgcaccgtg tccgggccgt
      481 gcgcggccgg caatgtcacc gacgcgcccg gctccaggcg cacgtgccag atcgcgacgt
      541 cggcctccgg gtgggccgcc caggaattcg gcggcggcgc cggcgcgtcg acaccgttga
      601 acgaacccgc gatcacggtg acgccgtcga cagtggggat gtcgtcggcc cacaacatcg
      661 tgaagtacgg gtcgacgcgc ttgtcggcgg cgggcaggtt gagccagatc tggaacagct
      721 cgatcgggtt cggttcgtcg cgatagagca acgggaacat ctcggagtgc acgatgccgt
      781 tcccggcggt cacccactgc acgtcgccgt gcccgaagcg cgccttcgcg ccgagcgagt
      841 cggagtggtc gatcaggcct tggcgcacga gcgtgaccgt ctcgaacccg cggtgcggat
      901 gctgcgggaa gcccggtacg acgtcgccgt ggtacatgct ccagccgtct cggcccgaga
      961 agtcctggcc gatgtcgcgg tccgcgagcg acgcgtccgg gccgaggtcg tcgttgccgc
     1021 gcgggtacgc gtcgtcgtgg tgcgcacaga agaggaacgg gtcgatcgtc ggccagcgcg
     1081 gcccgagcgg cacctgctcc acaacggggt cagtcatccc ggcaacggta gcgccgcatt
     1141 tgacgcttcc agtccccgcc gatcgaatac cgcccatgac gctggagctg ttcacgatga
     1201 gcattccgga gccgagtctc gtcgagctgt tctcgacgcg cgccgaggcc gaaggttggg
     1261 acggcatcac gttcaccgac tcgcagaacc tcgtcgccga cccgttcatc ggcatcgcga
     1321 tcggcgcgcg cgtcaccgaa cggctgcaat tcatgaccgg cgtctcgaac cccgcgacgc
     1381 gccaccccgc cgcgctggca accgtcgcgg caaccgtgca ggagacctcg ggcggacgga
     1441 tgacgctcgg tctcgggcgc ggcgacaccg ccctgttcca cctcggccgc catccccaat
     1501 cgctcaacga gttcttcgcg cgtaccgacg agttgcatac gtacctgaac ggcggtgtcg
     1561 tcgacatcga cgggtacccg agccagatcc ggtggctggc gcgcgctcag cagccgcgcg
     1621 ttccgatcga cattgcctcg tccggaccga aagtcatcgc gtacgccgcg cgcacggtcg
     1681 agcgcatcac gttcgcggtc ggtgcggatc cggagcgcat ggcgtgggca gtcgatctcg
     1741 cccgcgccgc gatgaaggat gccggccgca ccgacggcga cgtctcgttc ggcgcgtacg
     1801 tcaccgtcgc ctgtcatccc gacgcggcca aggcccgcgc gctggtgaag ggcagcgtcg
     1861 ccgccttcgc ccacttctcc gccatgccgg gctcgacggg cgcggggctc gacgaacgcg
     1921 accgcgagct cgtcgccgaa gtcggccgca cctacgacag caacatgcat ctcgtgaaca
     1981 gcgccgatca cgcgcaggtg ctgcccgacg agttcatcga tcgcttcgcc gtgatcggcg
     2041 acgcgagcgc ctgcgcgaag cgtctgcacg agctcgtgag cctcggcctg gaacggctcg
     2101 tgatcaccgg cccctcgttc ggtgccgacc gcgaagacgc caacacccac cgcacgctcg
     2161 tctgcgagga gctcctcccc gcgttgcgtt agttcgtcat cacgcgcgcg ggcgtgacga
     2221 gcaccgcgac gcggccttcg gcggccatga cgcggtcgta ctcgtcccag tcgtcgtggg
     2281 taccgcccgc cgccttgaag acggcgcgca acaactcggg cacgtcgacg ccggcgaccg
     2341 ggtcatcggg gccgatgatc tgcgcgggac cctcgacgct cacccactcc caaccgtcgc
     2401 ggaacgtgaa cgacgcccgg ccactgcggc ggatgttcgc gagcttcttc gcgctcccac
     2461 gtacgaccgc gccgacaacg ggcgcgccgt tcaccggatg gtcgagcaca cccgcgttca
     2521 ccaaggttgc gtgcacggaa ccgtcgggac gcgtcgtcgc gacgatcgtg agcccgtgac
     2581 tggcgccacc gaggcggcgt acgagatcga ggtcagccat gaacgtccag ggtacgttgc
     2641 cgggcatggc actcaccttg tgcgtgctcc tcgacgccgt gccgggcaac gagcagcagc
     2701 tgatcgacta cgaggatcag gtgctcgcgc tgatcccggc gcacggcgga cgagtcgtct
     2761 cgcgcgtccg cgcgctcgaa tccggcggtc cctacgaggt gcacgtcatc gagttcccat
     2821 ccgatgacgc gttggacgcc ttcatgagcg acccgcaacg gaccgcgctt gctccacaac
     2881 gcgaccgcgc aatcgcgcag acgacggtgt tacgcgtgga tcaggtgtag cgccactgct
     2941 tcgcgggtcc gcccgcggcg ccgtactcga ggcactgcac gtcgaaccag atcggcaaca
     3001 agaacttgaa gtagaacgcg tgcaccgtca ggttcggcgc cgcgagatcc tcgtagcgct
     3061 tgatctgcat ccggtcgtcg tcggccgcga acttctccag cttctcgagc acgtcgtcga
     3121 cctcggcgcg ggtgtcgagc aagaagccga gatggtcgta gccgggggac tccatccact
     3181 tcttgccctc cgccagcagg atgaactggc cgtcgtccgg caacaggatg tgcgtgtgct
     3241 gcccgacgac gtccgcgtcg gtcgcccgcc agccgaggat gtcgacgaag acactgtcga
     3301 cctcgcgccg gaactccttg gtaagcgtcc cgcgcgggaa cgtcaactcc atgtggttga
     3361 agcgcaacgg catggcgcca tcgtacgcgg gtcacggcat ccgcgacacg ggccggtccg
     3421 cgcgggtatc accttctgtc atggacacgc tccaaatggt cgaaccgccc gcgctgagcc
     3481 gcgaatcgga acgggtgcgc catctctcgg cgatgaagcg gcgcgccacc gggctgctgc
     3541 tcgtcatggc gatcgcattc gtcgtggtca ccttggtgac cgacgaggag ggcgtggccg
     3601 ggtacgtgca ggccgcgctc gaagcatcac tcgtcggcgg gctcgccgac tggttcgcgg
     3661 tcaccgcgct gttc
//
LOCUS       DASUGA010000283         7215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1397322, whole genome shotgun sequence.
ACCESSION   DASUGA010000283 DASUGA010000000
VERSION     DASUGA010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7215
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397322"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VFX21_05585"
     CDS             complement(<1..915)
                     /locus_tag="VFX21_05585"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5095462.1"
                     /translation="MSERLVVVGGDAAGMTAAATARRRATRDELEIIAFELGDYTSYS
                     ACGLPYFVADTIGDAQRLVARTPEEHRERGIDVRIRHEVVAIDLAARTVTARDTSGND
                     ERAEVVESFDKLLVATGASPIRPDLPGIDALGVHGIQSLTDGIDLRAHVREHLHADQH
                     AVVVGSGYVGLEMAEALKLRGLPVTIVDQGPQPMSTLDPDMGALVAATIRDEGITLRT
                     DTEVEGFETDADGHVRQVVTKDGKLPADLVVLGIGVRANSDLARDAGIAIGSNGGVTT
                     DAGMATSVDGVWAAGDCVESYHRVSRRHL"
     gene            complement(1046..2008)
                     /locus_tag="VFX21_05590"
     CDS             complement(1046..2008)
                     /locus_tag="VFX21_05590"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX5095463.1"
                     /translation="MKTRLTEMLGIEHPVMLAGMGGVSYHRLVAAVSEAGGIGTLGAA
                     PMSSEEMVREIELTREATNMPFGVDLLAALPDRLEPDARAIVKGGASLFVAGLGVPRD
                     IIKILHDGNVLVASMCGKVRHATAAVEAGCDFVIAQGTEAGGHTGQIATMPLVPQIVD
                     AVGDRVPVVAAGGLFDGRGLAAALTLGADGVWMGTRFIATPEARTVPGYKEALIRTPE
                     DGTVISRGFTGKTLRAVRNEWTQYIEEHPEELAKFPEQMARAFAANALHLGGDENADV
                     DPNRECYPAGQGVGAIEALIPAGDLVRSIVAEAERALDRANSFR"
     gene            2117..2623
                     /gene="purE"
                     /locus_tag="VFX21_05595"
     CDS             2117..2623
                     /gene="purE"
                     /locus_tag="VFX21_05595"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005461673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX5095464.1"
                     /translation="MSAPVVGVIMGSDSDLPVMQGAIDVLADFGVPYEVRIVSAHRTP
                     DVMVEYARGAADRGLRVIIAGAGGAAHLPGMTASLTPLPVIGVPVPLRQLDGLDSLLS
                     IVQMPAGIPVATVAVGNARNAGLLAVRILAIADDALRKAMSNYQDELDTTIREKDAEI
                     RTRFANGD"
     gene            complement(2733..4802)
                     /locus_tag="VFX21_05600"
     CDS             complement(2733..4802)
                     /locus_tag="VFX21_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5095465.1"
                     /translation="MLTQQATKTYRLVGITKFGSADSPLGASVTAFTLHEAQTINNYT
                     DQITSVRAVAEPGVSQTELQRRVEQAVKTSDPLTAADAEVITGKQLTEENQNDIQDAL
                     GFFNIALLVFAVVALFVAVFIIYNTFSIILAQRLRELALLRAIGAGTPQVFGSVMFES
                     FAIGLVAAILGFVAGIGLAVGLKNLLNVLGFGIPATSIVIPSTAIIASIIVGIGVTML
                     SALLPAWKAARVPPIAALRDVAFERSSGLARRALAGGIILFLGAATLLYGLFGGPSNA
                     IAFVGLGAGVMFIGVFVIAPLLSRPVGLILGAPHRIFGVSGALARENAMRSPRRTART
                     AAALMIGVSLVGFITIFAASAKESIAHAVDQQLKSDYILTGGTGFTSIFFSPTVGEEV
                     AKIPEFFAVSPVRIGEVRVEGKSEFITSSDPKPSEELIDFDVIEGAIGDLTDDGVAIS
                     EDYSDDHDLGMGDVVSMEFPDGAVVPLHVQAIYTRTQLAGDFLTSIGLYEQHFLPTQQ
                     LDFLVMAKLAPGADAKAAVAQMEKIVEPYPTVEIRDNEQYKKDQAASINQVVNIVYAM
                     LFLSIIIAFIGIVITLALSIYERTRELGLLRAVGMSRRQMRRTVRLESVLIALFGTAL
                     GLVVGLFFGYAVFLALRDEGFNRFAAAPGQLLIVVIVSVFVGIAAAIYPARRAARLDI
                     LESIATE"
     gene            complement(4893..5306)
                     /locus_tag="VFX21_05605"
     CDS             complement(4893..5306)
                     /locus_tag="VFX21_05605"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5095466.1"
                     /translation="MFRVTLKNISSHKLRVFFTALAAIIGVAFLSGTLVLTNTIQHTF
                     DDLFADVNRGTDVVVRGTEPFETQFSDQRRPLPESLVDVVKDVPGVEFAAPAVEVPYA
                     QVIGNDGKKIGNPGAGAPTFGVVWVDNDDLNPMSI"
     gene            complement(5314..6039)
                     /locus_tag="VFX21_05610"
     CDS             complement(5314..6039)
                     /locus_tag="VFX21_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015796838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5095467.1"
                     /translation="MTTAARAVDAMKVYGQGDTEVRALDGVTVDFPRSVFTAIMGPSG
                     SGKSTLLHCIAGLDRLTKGEVWIGDVNLNTLSEKELTRVRRDKIGFIFQAFNLIPTLN
                     AYENITLPMALAKRDPDKEWVDNVIDTVRLRDRLTHRPAELSGGQQQRVAVARALASR
                     PEIIFADEPTGNLDSRSGAEVLAFMRKAVDDFEQTIVMVTHDPISAANADRVIFLADG
                     RIVDEQQQPTADSVLSKMRELGD"
     gene            complement(6132..6986)
                     /gene="lgt"
                     /locus_tag="VFX21_05615"
     CDS             complement(6132..6986)
                     /gene="lgt"
                     /locus_tag="VFX21_05615"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX5095468.1"
                     /translation="MLLAYIPSPEHGVIDVGPLQLHMYGLMLAIGILVAGWIAQKRWR
                     AWGYPENTITEIAIPVVIGGVLGARIYHLFTGYSWDSGGIVGALKIWEGGLSIWGAVG
                     GGFIAALVMARVKHVDTLALMDALAPGVVIAQAIGRWGNWFNQELYGRPTKLPWGLEI
                     DNPPARYAQYKTFHPTFLYESLWCLAVFAVLVWAERRFRVKRGQTFAGYIALYTFGRI
                     FFEAMRSDPASRILGVRFNLLLSALLCVFGAILWVVYGRTQPVARASEHGDEKEPVPL
                     GDAPDPPA"
BASE COUNT         1204 a         2439 c         2410 g         1162 t
ORIGIN      
        1 caaatgcctg cggctgacgc ggtgatacga ctcgacgcaa tcgccggccg cccacacacc
       61 gtcgaccgag gtcgccatcc ccgcgtcggt cgtgacgccg ccattgctac cgatcgcgat
      121 gccggcgtcg cgcgcgaggt cgctgttggc gcgcacaccg atgccgagca cgacaagatc
      181 ggcgggcaac ttgccgtcct tcgtgacgac ctggcgcacg tgaccgtcgg cgtcggtctc
      241 gaagccttcg acctcggtgt cggtgcgcag cgtgatgccc tcgtcgcgaa tcgtcgctgc
      301 gacgagcgcg cccatgtcgg gatcgagcgt cgacatcggt tgtggtccct ggtcgacgat
      361 cgtcaccggc agcccgcgga gcttcagcgc ctcggccatc tccagaccga cgtagccgct
      421 gccgacgacg accgcgtgct gatcggcgtg caggtgctcg cgaacatgcg cgcgcaggtc
      481 gatgccgtcc gtcagcgact ggatgccgtg cacgccgagc gcgtcgatgc cgggcagatc
      541 ggggcggatc ggggacgcgc cggtggcgac caggagcttg tcgaacgact cgacgacttc
      601 cgcacgttcg tcgttcccgc tcgtatcgcg cgcggtgacg gtgcgggccg caaggtcgat
      661 cgcgacgact tcatgacgga tccggacgtc gatgccgcgc tcgcggtgct cctcgggcgt
      721 gcgggcgacc agccgttgcg cgtcgccgat cgtgtcggcg acgaagtacg gcagcccgca
      781 cgcggagtac gaggtgtagt cgccgagttc gaacgcgatg atctcgagct cgtcgcgagt
      841 cgcgcgccgg cgcgcggtcg cggccgcggt catgccggcc gcgtccccgc cgacgacaac
      901 gagccgttcg ctcacgcggg cggtggcctc ccgtcctccc gatgcgcgaa gcgccccgac
      961 aggaccgtct cggccactcg cggagtcttt cggcgggcca acaagccggg ctgcctcacg
     1021 cgagcacgtc gaaagcgaca aagagtcatc ggaatgaatt cgcgcggtcg agtgctcgtt
     1081 cggcctcggc cacgatgctg cgcacgagat cgccggccgg gatcagcgcc tcgatcgcgc
     1141 cgacaccctg gcccgcggga tagcactcgc gattcgggtc gacgtcggcg ttctcgtcgc
     1201 caccgaggtg cagcgcgttg gcggcaaacg cgcgcgccat ctgctcgggg aacttcgcga
     1261 gctcctcggg atgctcctcg atgtactgcg tccactcgtt gcggaccgcg cgcagggtct
     1321 tgccggtgaa gccgcgactg atcaccgtgc cgtcttcggg agtgcggatc agcgcctcct
     1381 tgtagcccgg caccgtgcgc gcctcgggcg tcgcgatgaa ccgcgtgccc atccagacac
     1441 cgtcggcgcc gagcgtcagc gcggcggcga ggccacgccc gtcgaacagg ccgcccgcgg
     1501 cgacgaccgg cacacggtcg ccgaccgcgt cgacgatctg cggcacgagc ggcatcgtcg
     1561 cgatctggcc ggtgtgcccg ccggcctcgg tgccctgcgc gatgacgaag tcgcaacccg
     1621 cctcgaccgc cgcggtcgcg tggcgaacct tgccgcacat gctggcgacg agcacgttgc
     1681 cgtcgtgcag gatcttgatg atgtcgcgcg gcacaccgag gccggcgacg aacaacgacg
     1741 cgccaccctt gacgatggcg cgcgcgtccg gctccaggcg atcgggcaac gccgcgagga
     1801 ggtcgacgcc gaagggcatg ttcgtcgcct cgcgcgtgag ctcgatctcg cgcaccatct
     1861 cctccgacga catcggcgcc gcgccgaggg tgccgatacc gcccgcttcg gagaccgccg
     1921 caaccagtcg gtggtaactc acgccgccca tcccggccag catcaccggg tgctcgatcc
     1981 cgagcatctc tgtcagtcgt gtcttcatgt tgatggctcc ctccactgcg agccacgcgg
     2041 cggctacgcc gcaagaaggc ctgcgttacg tccgcactcc ctcatcttcg cagaccgttc
     2101 ggttatcgtc ccaggcgtgt ccgctccggt cgtcggcgtg atcatgggca gcgattccga
     2161 cctgccggtg atgcaaggcg cgatcgacgt gctcgccgac ttcggtgtcc cctacgaagt
     2221 gcgcatcgtg tcggcgcacc gcacgcccga cgtgatggtc gagtacgcgc gcggcgccgc
     2281 cgatcgcggc ctgcgcgtga tcatcgcggg agcgggcggc gccgcccacc tgccgggcat
     2341 gaccgcttcg ctgacaccgt tgccggtgat cggcgtgccg gtgccgttgc gccagctcga
     2401 cggcctcgac tcgctgctgt cgatcgtgca gatgccggcc ggcattcccg tcgccacggt
     2461 tgccgtcggc aacgcccgca atgcgggcct cctcgcggtg cgcatcctcg ccatcgctga
     2521 cgacgcgctg cgcaaggcga tgagcaacta ccaggacgag ctcgacacca ccatccgcga
     2581 aaaggatgcg gagatccgca cccgcttcgc gaacggcgat taacctttgt cgctccgcac
     2641 gtgctcgcgt gaggcagccc ggattcttgg cccgcgctcc gctccgcgcg tgcctccgca
     2701 agctccggca ccgcgctgtt ggagcgagcg cgtcactccg tagcgattga ctcgaggatg
     2761 tcgaggcgcg ccgcgcgacg ggccgggtag atcgcggccg cgataccgac gaacaccgac
     2821 acgatcacga cgatcagcag ctgaccgggc gcggccgcga accggttgaa cccttcgtcg
     2881 cgcaacgcga ggaacaccgc gtagccgaag aacaggccga cgacgaggcc gagcgcggta
     2941 ccgaacaacg cgatcagcac cgactcgagg cgcaccgtcc ttcgcatctg ccgccggctc
     3001 atcccgacgg cacgcagcaa gcccaactct cgcgtgcgct cgtagatcga cagcgcgagc
     3061 gtgatgacga tgccgatgaa cgcgatgatg atcgacaaga agagcatggc gtagacgatg
     3121 ttcacgacct gattgatcga cgcggcctga tccttcttgt actgctcgtt gtccctgatc
     3181 tcgaccgtcg ggtacggctc gacgatcttc tccatctgcg cgaccgcggc tttggcatcg
     3241 gcacccggcg cgagcttggc catgacgagg aagtcgagct gttgggtcgg gaggaagtgc
     3301 tgctcgtaga gaccgatcga agtcaggaag tctccggcga gctgcgttcg cgtatagatc
     3361 gcctgcacgt gcagcggcac gaccgcgcca tcggggaact ccatcgagac gacgtcgccc
     3421 atgccgaggt cgtgatcgtc ggaatagtcc tcggagatcg cgacgccgtc gtcggtgagg
     3481 tcgccgatcg caccttcgat cacgtcgaag tcgatcagct cttcactcgg cttcggatcg
     3541 gacgaggtga tgaactcgct cttcccctcg acgcggacct cgccgattcg tacggggctg
     3601 accgcgaaga actcggggat cttggcgacc tcttccccga ccgtcggcga gaagaagatc
     3661 gaggtgaagc cggtaccgcc ggtcaagatg taatcgctct tcaactgttg gtcgacggcg
     3721 tgcgcgatcg actccttcgc cgacgcggcg aagatcgtga tgaagccgac gagtgacacg
     3781 ccgatcatca acgcggcggc ggtgcgcgca gtccgccgcg ggctgcgcat cgcgttctcg
     3841 cgcgcgagcg cgccggagac gccgaagatg cgatgcgggg caccgaggat cagacccacc
     3901 ggccgcgaca acagcggcgc gatgacgaag accccgatga acatgacccc ggcgcccaga
     3961 ccgacgaatg cgatcgcatt gctcggtccg ccgaacagac cgtagagcag cgtcgcggca
     4021 ccgaggaaga ggatgatgcc gcccgcgagc gcgcgtcgcg ccagacccga cgaccgctcg
     4081 aacgcgacat cgcgcagcgc ggcgatcggt gggacccggg ccgccttcca tgccggaagc
     4141 agcgccgaga gcatggtgac gccgatgccg acgatgatcg aggcgatgat cgcagtgctc
     4201 gggatgacga tggacgtcgc ggggatgccg aaaccgagca cgttcagcag gttcttgagc
     4261 ccgacggcga gcccgatgcc ggcaacgaac ccgaggatgg ccgcgacgag accgatcgcg
     4321 aacgattcga acatcacgga cccgaagacc tgaggagtgc cggcgccgat ggcacgcaac
     4381 aacgcgagct cccgcaagcg ctgcgccagg atgatcgaga acgtgttgta gatgatgaag
     4441 accgcgacga agagggcgac gacggcaaac accagcagcg cgatgttgaa gaagccgagg
     4501 gcgtcctgga tgtcgttctg gttctcttcc gtgagctgct tccccgtgat cacctcggcg
     4561 tcggccgcgg tcagcgggtc cgatgtcttg accgcttgct cgacgcggcg ctgcagctcg
     4621 gtctggctca cgcccggttc cgcgaccgca cgcacgctcg tgatctggtc ggtgtagttg
     4681 ttgatggtct gggcctcgtg cagcgtgaag gcggtgaccg acgcgcccag cggactgtcg
     4741 gcggacccga acttggtgat gccgacgagc cggtaggtct tggtcgcctg ttgcgtgagc
     4801 acttcgacgt cgtcgccgac ctcgaggtcg cctttgtcgg ctgtgccctt gtcgatcgtg
     4861 atctggttgt cggcgcgcgg cggttggcct tcttagatcg acatcgggtt gaggtcgtcg
     4921 ttgtcgaccc agacgacgcc gaacgtgggc gcgcccgccc cgggattccc gatcttcttg
     4981 ccgtcgttgc cgatgacctg cgcgtacgga acctcgactg cgggtgccgc gaattccacg
     5041 cccgggacgt ccttgacgac gtcgacgagc gactccggca acgggcgacg ctggtcgctg
     5101 aactgcgtct cgaacggctc ggtgccgcgc acgacgacat cggtgccccg gttcacgtcg
     5161 gcgaagaggt cgtcgaacgt gtgctggatc gtgttcgtca ggaccaacgt gcccgacagg
     5221 aacgcgacgc cgatgatggc ggcgagcgcg gtgaagaaca cccgcagctt gtgcgacgag
     5281 atgttcttga gcgtgaccct gaacatcgtg ccgtcagtcc ccgagctctc gcatcttcga
     5341 gagcacggag tccgcggtcg gctgctgttg ctcgtcgacg atgcgaccgt cggcgaggaa
     5401 gatgacccgg tccgcgttgg cggcggagat cggatcgtgg gtcaccatga cgatcgtctg
     5461 ctcgaagtcg tcgaccgcct tgcgcatgaa cgcgagcacc tcggcgccgg aacgcgagtc
     5521 gaggttgccc gtcggctcgt cggcgaagat gatctcgggc cggctggcca gggcgcgagc
     5581 aacggcgacg cgctgctgct ggccgccgga gagctcggcg gggcggtgcg tcaaccggtc
     5641 acgcaaccgc acggtgtcga tcacgttgtc gacccactcc ttgtcgggat cgcgcttggc
     5701 cagcgccatc ggcagcgtga tgttctcgta ggcgttcagc gtcgggatga ggttgaacgc
     5761 ctggaagatg aacccgatct tgtcgcggcg aacgcgtgtc agctccttct cggagagcgt
     5821 gttcaagttg acgtcgccga tccacacctc acctttcgtg aggcggtcga gcccggcgat
     5881 gcagtgcagc aacgtcgact tgccggaccc cgacgggccc atgatcgcgg tgaacaccga
     5941 gcgcgggaag tcgacggtca cgccgtcgag cgcgcgcacc tcggtgtcgc cctggccata
     6001 gaccttcatg gcgtcgaccg cgcgcgcagc cgttgtcatc gtctccggga cagtggacgt
     6061 cacggtgtgc tcctctgaat accgtcgcag cggcgtccac gctagggaac gcgcggacgg
     6121 attacatccc tctaggcggg tgggtcgggc gcatccccta agggaaccgg ttctttctcg
     6181 tccccgtgct cggaggcccg ggccaccggc tgtgtccggc cgtacaccac ccacagaatc
     6241 gccccgaaga cgcacagcag ggccgagagc agcaggttga accggacacc gaggatcctc
     6301 gaggccggat ccgagcgcat ggcctcgaag aagatacgac cgaacgtgta gagcgcgatg
     6361 tagcccgcga acgtctgccc gcgcttgacg cggaagcgtc gttccgccca gacgaggacg
     6421 gcgaagacag ccaggcacca gagcgactcg tacaggaacg tcgggtggaa cgtcttgtac
     6481 tgcgcgtatc gcgcgggcgg gttgtcgatc tccaatcccc atggcagctt cgtcggccgg
     6541 ccgtagagct cttggttgaa ccagtttccc caacgcccga ttgcctgcgc gatgacgaca
     6601 ccgggcgcga gcgcgtccat caacgcgagc gtgtccacgt gcttcacgcg cgccatgacc
     6661 agcgcggcga tgaagccgcc gcccaccgcg ccccagatcg acaggccgcc ttcccagatc
     6721 ttcaatgcgc cgacgatgcc gccactgtcc cagctgtagc cggtgaacag gtggtagatg
     6781 cgggcgccga gcacgccacc gatcacgacc gggatcgcga tctcggtgat cgtgttctcg
     6841 gggtagcccc acgcgcgcca ccgtttctgg gcgatccaac ccgcgaccag gatgccgatc
     6901 gcgagcatca agccgtacat gtgcaactgg agcggaccga cgtcgatgac gccgtgttcc
     6961 gggctcggga tgtaggcgag cagcatgacg ggccaatgct atggggccgt ccctcggccg
     7021 gaccgagcgg tcgatttgcg acggtaaaat gcctgcatgg ctcgcaccga cgcgaatccg
     7081 ccgatgccgg agttctccac cgactacgtg ctgcaagagg tcgaccccaa gtggctcacc
     7141 accgcggtca aacccaagca gttcctccac atcgaccaag ccgaatgcat cctctgcgaa
     7201 ggctgcgtcg acatc
//
LOCUS       DASUGA010000284         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_147203, whole genome shotgun sequence.
ACCESSION   DASUGA010000284 DASUGA010000000
VERSION     DASUGA010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147203"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1146
                     /locus_tag="VFX21_05620"
     CDS             157..1146
                     /locus_tag="VFX21_05620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX5095469.1"
                     /translation="MSAPPDNLGVTFASLQALGLDAALTTAKWAEDLGYQSYWTAETT
                     GPEAFSTLAAVGAVAPSLDLGTGVLALQLRTPPLTAMAAATLQALRPDRDIVLGIGIS
                     SPVVTSQWHGAPYGDRPLARVREFVELTRQCLSGDSVTFKGDFYEVRRFRLGVKLGDR
                     KPKIVIGALNERMLQLAGEVADGVLLNYLPASLVPWSVEQVRKGGDATIYAYVHVGVC
                     DREDGIDYARRDLFSYAVVDSYARNFERAGFADEVADIRERHGAKDREGALAAVSDAM
                     VDAIDVMGDADHVKATVRAYIDAGVEAPIVMPLPWGQDRLGVVRATLEAAAQA"
     gene            1183..1959
                     /locus_tag="VFX21_05625"
     CDS             1183..1959
                     /locus_tag="VFX21_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008229570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5095470.1"
                     /translation="MEVSGKVVVITGGASGIGRALAERIAADGASAVIVADIDEAGAN
                     AVADRIGAVGRRVDVADEADIARLIDETERQQGPIDLFCSNAGILAIGGAEQSDDTWR
                     RCIDVNVMAHVWAARHLVPRMLERGGGYLLNTASAAGLLIQIGSAPYTVTKHAAVGFA
                     EWLAVTYGEQGLRVSVLCPQAVETKMTAGFEGGGVAGVDGMLAPEAVADAVIDGLRDE
                     RFLILPHPEVAEYERRKTADRDRWLTGMQRLQARFGDMLS"
BASE COUNT          316 a          703 c          692 g          293 t
ORIGIN      
        1 tcgacccgca gcagtgtcgg tcgacgaccc gaactgctgg acgagcgacc gcgccgaagt
       61 ggtcacgcgc gtgagctggg tcggcaccgc ccgcaccgcc gagccctacg agctacggcg
      121 ggacggctcg ttctgacggc aggtaacgtc gcccggatga gcgcaccgcc cgacaacctc
      181 ggcgtcacct tcgccagctt gcaggcactc ggtctcgacg ccgcgctgac caccgccaaa
      241 tgggccgaag acctgggcta ccagtcgtac tggacagccg agacgaccgg ccccgaagcg
      301 ttcagcacgc tcgccgcggt gggtgcggtc gcaccatcgt tggatctcgg caccggtgtg
      361 ctggcgctcc agttgcgcac gccgccgctc accgccatgg ctgctgccac cttgcaggcc
      421 ctccgacccg atcgcgacat cgtgctcggc atcggcatct cctccccagt cgtcacgagc
      481 cagtggcacg gcgcccccta cggcgaccgg ccgctcgccc gcgtgcgcga gttcgtcgag
      541 ctcacgcgcc aatgcctctc aggtgattcg gtcacgttca agggagactt ctacgaggtc
      601 cgccggttcc gactcggcgt gaagctcggc gaccgcaagc cgaagatcgt catcggtgcc
      661 ctcaacgagc gcatgctgca actcgcaggc gaagtggccg acggggtgct gctgaactac
      721 ctgcccgcct cgctcgtccc gtggtcggtc gaacaggtgc gcaagggcgg cgacgccacg
      781 atctacgcat acgtgcacgt cggcgtctgc gatcgggagg acggcatcga ctacgcgcgt
      841 cgcgatctgt tctcgtacgc ggtggtcgac tcgtacgccc gcaacttcga acgcgcgggc
      901 ttcgccgacg aagtcgccga catccgcgaa cgccacggtg ccaaggaccg cgaaggcgcg
      961 ctggccgcgg tctcggacgc gatggtggat gccatcgacg tgatgggcga cgccgatcac
     1021 gtgaaggcga cggtccgtgc gtacatcgac gcaggtgtcg aggcgccgat cgtgatgccg
     1081 ctgccgtggg gacaggaccg cctcggcgtg gtgcgagcca ccctcgaagc ggccgcccag
     1141 gcgtgactcc cgcaccctcg tcgcgcacgt agcattcgct cgatggaggt ctccggcaag
     1201 gtcgtcgtca tcacgggcgg agcgtcgggc atcggtcgag cgctggcaga acgcatcgcg
     1261 gccgacgggg cgagcgcggt gatcgtggcc gacatcgacg aagccggtgc gaacgccgtg
     1321 gccgaccgca tcggcgcggt cggccggcgc gtcgacgtcg ccgacgaggc ggacatcgcg
     1381 cggttgatcg acgagaccga gcgccagcaa ggcccgatcg acctgttctg ctcgaacgcc
     1441 ggcatcctcg ccatcggcgg cgcggagcag agcgacgaca cgtggcgacg atgcatcgac
     1501 gtcaacgtca tggcccacgt gtgggccgcc cgccatctcg tcccacgcat gctcgaacgg
     1561 ggcggcggct acctcctcaa caccgcgtcg gccgcgggct tgctgatcca gatcggctcc
     1621 gcgccctaca ccgtcaccaa gcacgccgcg gtcgggttcg ccgaatggct cgccgtcacc
     1681 tacggcgaac agggcctgcg cgtgtcggtg ctctgcccgc aagccgtcga gacgaagatg
     1741 accgcgggct tcgaaggcgg cggcgtcgcc ggggtcgacg ggatgttggc gcccgaagcc
     1801 gtggccgacg cagtgatcga cgggctgcgc gacgagcgct tcctcatcct tcctcacccc
     1861 gaggtggccg agtacgaacg acgcaagacg gccgatcgcg atcggtggct tacgggcatg
     1921 cagcggctgc aggcgcggtt cggggacatg ctgtcgtgac gcacggaggt gacgacctca
     1981 ccatgctcgg gttgcggcgg ggcg
//
LOCUS       DASUGA010000285         2239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1171229, whole genome shotgun sequence.
ACCESSION   DASUGA010000285 DASUGA010000000
VERSION     DASUGA010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171229"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(449..1543)
                     /gene="proB"
                     /locus_tag="VFX21_05630"
     CDS             complement(449..1543)
                     /gene="proB"
                     /locus_tag="VFX21_05630"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX5095471.1"
                     /translation="MSGTLGVVKVGTSSITNARGELDDTALLKLCADLAAARATGDRV
                     VLVMSGAIAAGMPALGLHARPTDIGTLQALAAVGQPRLVERMNAMLSAHDIVAGQVLL
                     TPYDFAHRSQYLHARETLQHLLDLGVLPIVNENDTVADDEIRYGDNDRLAALVAHLLH
                     ADALVLLTDTAGLYTADPRLDEGASLIEEIVEVDDELEAAAGGAGTSRGSGGMASKLV
                     AAKIAAWSGVRAVIAAASAPNVVTDALGGVPVGTAFAPRPHRLPARKLWIAFAQGAVG
                     RVVVDDGARSALVEGNKSLLAAGVRGVDGEFEADAPVEIVGEDGEPFAKGLSRYSSRQ
                     LRSVMGRRTSDLPEGLPHEAVHRDDLVVLP"
     gene            complement(1540..>2239)
                     /gene="cgtA"
                     /locus_tag="VFX21_05635"
     CDS             complement(1540..>2239)
                     /gene="cgtA"
                     /locus_tag="VFX21_05635"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HEX5095472.1"
                     /translation="VSAAKPKIANYPFTTLEPHLGVVRFGEHEFVVADIPGLIEGAAE
                     GRGLGYQFLRHVERARVLVILCDLAPVDERSPQEQERILLDELGRYQPELLERPRLVI
                     GTKADIATHDFDGPRISAVTREGLDEVLGRLAELVRAARETEPAEEPYVLLRPEEQGF
                     SVVREGAHEWRVKGKPAERAVALADLTNIEALAYVQQRLRRLGVERALTRAGVQDGDT
                     VRIGDHELEYEAGL"
BASE COUNT          328 a          787 c          794 g          330 t
ORIGIN      
        1 gtcaccagcg gtgaggcgtt cacgacgtag atctgctcga cgctgcctcg gatcgtcagg
       61 ccggtcgggt aggagggcac cgcgttgacc gaaccggcag tcgtgagcac gccacctgcg
      121 aggagcgcgg cgagcgcgag cacgaccaga cgggaacggg atcgatacat cggtccacct
      181 cacacttctg cgcgccggtc accccgacgg cggtcgggaa cctagcgggc gcgcgtcgcg
      241 ttgtcttcac ctccgagctg aagttgcgtg gcgttcacct cacggccggc cgactccggc
      301 tccacgcgca tggacggcgc gagtgaccga tgcgtcatcg ggatatcgga atggccagtc
      361 ggtcacacct cgccgccgac ccgatagcgc gtgccagctt cgttgtcgcg aacggagcac
      421 ccggcatcag cggcgccgac cgacgaagct acggcaggac gaccaagtcg tcgcggtgga
      481 cggcctcgtg gggcagccct tcggggaggt cggacgtacg gcgacccatg acgctgcgga
      541 gttggcgcga cgaatagcgc gacaagccct tggcgaacgg ttcgccgtct tcgccgacga
      601 tctcgacggg cgcgtcggct tcgaactcgc cgtcgacgcc gcgcacgccc gcggcgagga
      661 gcgacttgtt gccctcgacc agcgcactgc gcgcgccgtc gtcgacgacg acacggccga
      721 ccgcgccctg cgcgaacgcg atccagagct tgcgggccgg caaccggtgc gggcgcgggg
      781 cgaatgcggt accgacgggg acaccgccga gcgcatcggt cacgacgttc ggggcgctcg
      841 cggccgcgat gacggcacgc acgcccgacc acgccgcgat cttcgcggcc acgagcttgc
      901 tcgccatgcc gccgctgccg cgcgaggtgc ccgcgccccc ggccgcggct tcgagctcgt
      961 cgtcgacctc gacgatctcc tcgatgagcg acgcgccttc gtcgaggcgc ggatcggcgg
     1021 tgtacagacc ggccgtgtcg gtcaacagga cgagcgcgtc ggcgtgcagc aagtgcgcga
     1081 cgagcgcggc gaggcggtcg ttgtcgccgt agcggatctc gtcgtcggcc accgtgtcgt
     1141 tctcgttgac gatcggcaac acgccgaggt cgagcagatg ctgcagcgtc tcgcgcgcgt
     1201 gcaggtattg actgcggtgc gcgaagtcgt acggcgtcag cagcacctga ccggcgacga
     1261 tgtcgtgcgc cgacaacatc gcgttcatgc gttcgaccaa ccggggctgc ccgacggccg
     1321 ccagcgcttg cagcgtgcca atgtcggtcg gccgggcgtg cagaccgagg gcgggcatcc
     1381 ccgccgcgat cgcgccggac atcacgagga cgacgcgatc gccggtcgcg cgcgcggcgg
     1441 ccaagtcggc gcacaacttc aacagcgcgg tgtcgtcgag ctcgccgcgc gcgttcgtga
     1501 tcgacgacgt gccgaccttg acgacaccga gcgtgccgct cacaaccccg cctcgtactc
     1561 gagctcgtgg tcgccgatgc gcaccgtgtc gccgtcttgc acacccgcgc gcgtgagcgc
     1621 gcgctcgacg cccagccggc gcaatcgttg ctgcacgtac gcgagtgctt cgatgttcgt
     1681 gaggtccgcc agtgcgaccg ctcgctccgc gggcttgccc ttgacccgcc actcgtgcgc
     1741 gccttcgcgc acgacggaga agccttgctc ctcgggacgc agcagcacgt acggctcctc
     1801 ggccggttcg gtctctcggg cggcccggac gagctcggcg agacggccca gcacttcgtc
     1861 gagcccctcg cgggtcaccg cggagatgcg cgggccgtcg aagtcgtggg tcgcgatgtc
     1921 ggccttcgtg ccgatcacga gccgcggccg ttcgagcagc tcgggctgat agcgcccgag
     1981 ctcgtcgagc aggatgcgct cctgttcctg cggcgatcgt tcgtcgaccg gcgccaggtc
     2041 gcagaggatg acgagcacgc gcgcgcgttc gacgtgccgc aggaactggt agccgaggcc
     2101 gcggccctcg gccgcgcctt cgatcaagcc ggggatgtcg gcgacgacga actcgtgctc
     2161 gccgaaccgg acgacgccga ggtgcggctc gagcgtggtg aacgggtagt tggcgatctt
     2221 cggcttggct gcgctgacc
//
LOCUS       DASUGA010000286         7025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1421947, whole genome shotgun sequence.
ACCESSION   DASUGA010000286 DASUGA010000000
VERSION     DASUGA010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7025
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421947"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..168
                     /locus_tag="VFX21_05640"
     CDS             <1..168
                     /locus_tag="VFX21_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5095473.1"
                     /translation="GNTSFENASFDTSIESIDRRLFTLPADMLVLPGHGLDTTIGEER
                     PHLDEWITRGW"
     gene            complement(232..735)
                     /locus_tag="VFX21_05645"
     CDS             complement(232..735)
                     /locus_tag="VFX21_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX5095474.1"
                     /translation="MRTSTVDDVVTLQWPEDRVRLDELAAHDMPRLLLVAPGAEPPEH
                     RDERSDWMRLPAEGSEVRARVDALRRRLEHPGEPYLDSFGVLHRGERWVSLPPIEERI
                     ADLLVVRAGRVVGRRTLARAAWPEGVPNDRAMDSRMMLLRRRVAPLGLRIHTVRRQGF
                     LLEIDPR"
     gene            complement(801..3527)
                     /locus_tag="VFX21_05650"
     CDS             complement(801..3527)
                     /locus_tag="VFX21_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5095475.1"
                     /translation="MHDIDIGTVLTHILVVLVAAKLAAEISERVGIPAVVGEIIAGIL
                     IGPSVLDIVGSDEVLRVLGELGVILLLLEVGLEMDIVELGRVGRTSLFVAVVGVVAPL
                     LLGLGAMELIGDDFNTSLFIGAALTATSVGITARVFGDLRALATTEARIVLGAAVADD
                     VIGLVVLTVVVRLVTEDSVSLVSVGGIVLVAVAFLVVGGVVALRVAPPLFSFVERISR
                     SAGTMVAIALAFTLAFAQLADAAKLAPIVGAFVAGLALSRSRESDRIRRELAPVGHLF
                     IPVFFLQIGIDADISAFGRAEVLRDAAILLAVAVVGKMLSPVGAIGSTGDKLLIGLGM
                     LPRGEVGLIFASIGLANNVFDENLYAALLLVVLATTLVTPILLRWRSQRLRAGHHEPD
                     VSEPEPAEGWLEVDDGEIVLPARPPGRLALQLAFETAVLAASYPPSAKLLDWFAAHRD
                     APITWTSGATKSLLDVVRHGNVRSWRFLDALGILERALPELSESLAARRSDPMMLDPT
                     GLHRWITLDRLLEQTETGPLAAERAKLQFPERLLLAAVLVDALEGRDGIVPLTRRVVG
                     RLGLGAAAEQEIALLVGDVHLLWDMALRVDSLQEENVLQLAAHLDSPETARAAYLLAL
                     AVQRRDSWEQDRLETLYDYLQRVLADPELGGLEARNLVSRQRAEAARLLNGNALAIER
                     IEHAPRSYLLAQTPSAMARAATLLTRAQGRRTVAVQCEPGDVDGEWFVDVAASDRPGL
                     LATITGVLAELGVDVQRAVIATWSDGGALDSFVVTARSVPVPAELERAITERIGRVAP
                     TEPVEGAKVDFDDGASPWHTVCEVRASDRPGRLHEIATAFAAANVSVHAARLSVEHDT
                     IVDRFEVTGPGGTKLDATHKERFADFLHSGVKPRRRRVPRLLLAAVGQKAFI"
     gene            complement(3619..5130)
                     /locus_tag="VFX21_05655"
     CDS             complement(3619..5130)
                     /locus_tag="VFX21_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX5095476.1"
                     /translation="MLVSALAAAFVVVGALPAFAQAETPDPTAVLGTQVNLLWVIIGA
                     VLVIFMQAGFALVETGFCRAKHAAHVVSTNFAIFGLGFVAYYLVGYAFMFGGWTIPAV
                     GYDTPVGTHLVGSGNWVFLWQGGWAGSGEAFYTAGTMGIFLYMVAFMDTVATIPTGAM
                     AERWKWKAFVGWGLFCGAIYYPLFGAWTWGGGWLAKLGESAEWGVGYADFAGSGVVHA
                     VGGAAALAGAIVLGPRIGKYGKDGKPRTIPGHHIPMAMLGTFILLFGWFGFNAASTFA
                     VTDIRFAVVATNTAIAAAFGAVAAMFFMMFKGEKPDPGMMCNGMLAGLVAITAPCAFV
                     QPWAAMVIGIIAGCLVIVAARFIENRGVDDPVGAVAVHGVCGTFGVLAVGIFADGQYG
                     AGWNGTTTTTDGVTGILYGGDGPGQFLAQLAGAVVIWTVIFGVAFLFFKIQNAWMKGG
                     IRPSAEEELEGMDIPEMGAYAYPEFMHPELFDHLPEGHGSPTEIAPHGDEIKV"
     gene            complement(5406..5732)
                     /locus_tag="VFX21_05660"
     CDS             complement(5406..5732)
                     /locus_tag="VFX21_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX5095477.1"
                     /translation="MHTLASRVVAPAEPHIDEFGVLHRGTQWVALPPIEERLASVLLL
                     REGYVVGRRTLARAAWPTNRAPKERSIDSRISLLRRRVAPLRIRIRTVRGQGFVLEVE
                     ERSPAS"
     gene            complement(5778..6140)
                     /locus_tag="VFX21_05665"
     CDS             complement(5778..6140)
                     /locus_tag="VFX21_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095478.1"
                     /translation="MRRVRLLLLAAAFLAAVSQLFALARMVDDRMRPVAETVQDDRAT
                     ITARVTSAPAVVASVTQRRRDLRTAVMVLGFAAALFAALLRRGSRQRALATLTPRLRL
                     HGPPRAVRRGPPHLHVTA"
     gene            complement(6310..>7025)
                     /locus_tag="VFX21_05670"
     CDS             complement(6310..>7025)
                     /locus_tag="VFX21_05670"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5095479.1"
                     /translation="GGPVTVKALQDGDIDVGLLFTGSSVIDDDFVLLEDDKGLQPADN
                     PTAVINKDKATDDVTSIIDEVNGKLTLDEYNTMALSVFNDKEDPADVAGAFLERNGLT
                     EAGTTGEGTSLTVGSKDFAGAQLLSQAYGQALAAKGYDISYKDNIGPTETVYPLVKDG
                     TIDLYGEYTGTLLTYLEGTPTADSQETYDALVEKLSADNLTATAPSSAQDVNGFYVTT
                     ETADKYGLTKVSDLTQPAS"
BASE COUNT         1200 a         2393 c         2422 g         1010 t
ORIGIN      
        1 ggcaacacct cgttcgagaa cgcgagcttc gacacgagca tcgagtcgat cgaccgccgg
       61 ctgttcacac tcccggccga catgttggtg ctccccggcc acggcctcga cacgacgatc
      121 ggcgaggagc gcccgcacct cgacgaatgg atcacccgcg gctggtaacg ccaacaacgc
      181 tcgggcgtct tacaggggcg gattccgccc ttgtaagacg cccgtagtgt ttcagcgggg
      241 gtcgatctcc aacaggaagc cttggcggcg cacggtgtgg atgcgcaggc cgagcggcgc
      301 gacgcggcgg cggagcagca tcatgcggga gtccatcgcc cgatcgttgg gcacgccttc
      361 gggccaggcc gcccgcgcga gcgtgcggcg gccgaccacg cgaccggcgc gcacgacgag
      421 caggtcggcg atgcgctcct cgatcggagg caggctgacc cagcgctcgc cgcggtgcaa
      481 cacgccgaag ctgtcgagat agggctcgcc cggatgctcg agccgccggc gcagcgcgtc
      541 gacgcgcgct ctgacctcgc tcccctccgc gggcagccgc atccagtccg acctctcgtc
      601 ccgatgctcg ggcggttcgg cgcccggcgc gaccaacagc aaccgcggca tatcgtgggc
      661 ggccagctca tcgagtcgga cccggtcctc gggccactgc aaggtgacga cgtcgtcaac
      721 ggtgctcgtt cgcattgcct cacgacccta cgttcgctgt gtcgcggcaa tgtctgcggc
      781 gcgttacggc cacactgcag ctagatgaag gctttctgac ccacggccgc cagcagcaac
      841 ctggggacgc gccgccgccg cggcttcacc ccggagtgca ggaagtcggc gaagcgctcc
      901 ttgtgggtgg catcgagctt ggtcccaccc ggcccggtga cctcgaaacg gtcgacgatc
      961 gtgtcgtgct cgaccgagag ccgggccgcg tgcacgctca cattggccgc cgcgaacgca
     1021 gtcgcgatct cgtggagccg gcccggacgg tcactcgcgc gcacctcgca gaccgtgtgc
     1081 cacggcgatg cgccgtcgtc gaagtcgacc ttggcgccct cgaccggttc cgtcggcgcg
     1141 acgcggccga tccgctcggt gatcgcgcgt tcgagctcgg cgggaaccgg caccgaccgc
     1201 gcggtgacga cgaaggagtc gagggccccg ccgtcggacc acgtggcgat gaccgcgcgc
     1261 tgcacgtcga cgccgagctc ggccagcacg ccggtgatcg tcgcgagcag accgggacga
     1321 tcggacgccg cgacgtcgac gaaccactca ccatcgacat cgccgggctc gcattgcacc
     1381 gcaacggttc gccggccctg cgcgcgcgtg agcaacgtcg ccgcgcgcgc catcgccgac
     1441 ggcgtctgcg cgagcaggta gctgcgcggc gcatgttcga tgcgctcgat cgcgagcgcg
     1501 ttgccgttga gcaggcgggc cgcttccgcg cgctggcgac tgactagatt gcgcgcctcc
     1561 agcccaccga gctcggggtc ggcgagcacg cgctgcaggt agtcgtacaa cgtctcgagc
     1621 cgatcctgct cccacgagtc gcgacgttgg acggcaagcg ccaggaggta cgcggcgcgt
     1681 gcggtctccg gcgagtcgag atgtgcggcg agctgcagca cgttctcctc ttgcagagag
     1741 tcgacgcgca gtgccatgtc ccacaacaga tggacgtcgc cgacgaggag cgcgatctcc
     1801 tgttcggctg cggcaccgag cccgaggcgg ccgacgacgc ggcgagtgag cgggacgatg
     1861 ccgtcacgtc cttcgagagc gtcgacgagc acggccgcca gcaggaggcg ctcggggaac
     1921 tgcagcttcg cgcgctcggc cgcgagtggc cccgtctcgg tctgctccaa caggcggtcg
     1981 agcgtgatcc accgatgcaa cccggtcggg tcgagcatca tcgggtcgct gcgacgcgcg
     2041 gccagcgact ccgacagttc cggcagcgcg cgttccagga tgccgagcgc gtcgaggaac
     2101 cgccaggacc gtacgttgcc atggcggacg acgtcgagca gcgatttcgt cgcgccgctc
     2161 gtccacgtga tcggcgcgtc gcggtgcgcc gcgaaccaat cgagcaactt ggccgacggc
     2221 gggtacgacg ccgcgagcac ggccgtctcg aacgcgagct gcaacgccag gcggccgggc
     2281 ggccgcgcgg gcagcacgat ctcaccgtcg tccacctcga gccaaccttc cgcgggctcg
     2341 ggttcggaga cgtcgggttc atggtgaccg gcacgcagtc tctgcgaccg ccagcgcagg
     2401 agtatcgggg tgaccaaggt cgtcgcgagc acgaccagca gcagcgccgc gtacaggttc
     2461 tcgtcgaaca cgttgttggc gagtccgatc gaggcgaaga tcagcccgac ttcgccgcgc
     2521 ggcagcatgc cgagcccgat gaggagcttg tcaccggtac tgccgatcgc gccgaccggc
     2581 gacagcatct tgccgacgac ggcaacggcc aggaggatcg cggcgtcgcg caggacctcc
     2641 gcccggccga acgcgctgat gtcggcgtcg atgccgatct gcaggaagaa gacggggatg
     2701 aagaggtgac cgacgggcgc gagctcgcgg cggatgcgat ccgactcccg gctgcgggaa
     2761 agtgcgaggc cggcgacgaa ggcgccgacg atcggcgcga gcttggccgc gtcggcgagc
     2821 tgcgcgaacg cgagcgtgaa cgcgagggcg atggcgacca tcgttcccgc cgagcgcgag
     2881 atgcgctcga cgaacgagaa caacggcggg gcgacccgga gcgcgaccac cccgcccacg
     2941 acgaggaacg cgaccgcgac gaggacgatg ccgccgacac tgacgagcga gacggagtcc
     3001 tcggtgacga gacggacgac cacggtcagc acgacgaggc cgatgacgtc gtcggcaacg
     3061 gccgcgccga gcacgatgcg cgcctcggtg gttgcgagcg cgcggaggtc gccgaacacg
     3121 cgcgccgtga tccccacgct ggttgccgtc aacgcggcgc cgatgaagag cgacgtgttg
     3181 aagtcgtcac cgatgagctc catcgcgccc aggccgagaa ggagcggcgc gacgacgccg
     3241 accaccgcga cgaacagcga cgtccgaccg acgcggccga gctcgacgat gtccatctcg
     3301 agcccgacct cgaggagcag caggatcaca cccagctcgc cgaggacgcg cagcacttcg
     3361 tcggagccca cgatgtcgag caccgacggc ccgatcagga tgcccgcgat gatctcgccg
     3421 acaacggccg ggataccgac gcgctcggag atctcggcgg ccagcttcgc cgcgacgagc
     3481 acgacgagga tgtgcgtgag aacggtgccg atgtcgatgt cgtgcaacgg aagacctaac
     3541 gggtccggcc gcgacaccgg acgggcactt cagtgtgccc gtccggtcgt cacgccgtag
     3601 gtgattgctc gggaagtctc agaccttgat ctcgtcgccg tgcggcgcga tctcggttgg
     3661 gctcccgtgc ccttcgggca ggtggtcgaa caactccggg tgcatgaact cggggtaggc
     3721 gtaggcgccc atctcgggga tgtccatgcc ttcgagctct tcctcggccg acgggcggat
     3781 gccgcccttc atccaggcgt tctgaatctt gaagaagagg aatgcgactc cgaagatcac
     3841 cgtccagatg accacggcgc cggcgagctg ggcgaggaac tgtcccggcc cgtcaccgcc
     3901 gtagaggatg cccgtgacac cgtcggtcgt cgtcgtggtg ccgttccaac ctgcgccgta
     3961 ctggccgtcg gcgaagatgc cgaccgcgag cacaccgaag gtcccgcaca caccgtgcac
     4021 cgcgaccgca ccgaccgggt cgtcgacgcc gcggttctcg atgaagcggg cggccacgat
     4081 gacgaggcaa ccggcgatga tgccgatgac catcgcggcc cacggctgca cgaacgcgca
     4141 gggcgccgtg atcgcgacga gaccggcgag catgccgttg cacatcatgc cggggtcggg
     4201 cttctctccc ttgaacatca tgaagaacat cgcggcgacg gcaccgaagg cagctgcaat
     4261 ggccgtgttt gtcgcgacga ccgcgaaccg aatgtccgtg acggcgaacg tggacgcggc
     4321 gttgaagccg aaccagccga acagcaggat gaacgtgccg agcatcgcca tcgggatgtg
     4381 gtgccccggg atggtgcgcg gcttgccgtc cttgccgtac ttgccgatgc gcggcccgag
     4441 gacgattgcg cccgcgagcg cggcggcacc accgactgcg tgcacgacac cggaaccggc
     4501 gaagtcggcg taaccgacgc cccactcggc gctctcaccg agcttcgcga gccaaccgcc
     4561 gccccacgtc cacgccccga acaacgggta gtagatcgcg ccgcagaaca ggccccagcc
     4621 gacgaacgcc ttccacttcc accgctcggc catcgcgccg gtcgggatgg tcgcaaccgt
     4681 gtccatgaac gcgaccatgt agaggaagat gcccatcgtg ccggcggtgt agaacgcctc
     4741 cccgctgccg gcccagccgc cctgccacag gaacacccag ttcccggagc cgacgaggtg
     4801 ggtaccgacc ggcgtgtcgt agccgaccgc gggaatcgtc cagccgccga acatgaacgc
     4861 gtacccgacg aggtagtacg cgacgaaccc cagtccgaag atcgcgaagt tcgtcgacac
     4921 gacgtgcgcc gcgtgcttcg cacgacaaaa gccggtctcg acgagcgcga aacccgcctg
     4981 catgaagatg acgagcaccg cgccgatgat gacccagagc aagttgacct gcgtccccag
     5041 caccgcggtc ggatcgggtg tctctgcttg cgcaaatgca ggcaacgcgc cgacgaccac
     5101 gaacgccgcg gcgagggccg acaccagcat cagcttccgt cgaagtctca cggcgcaccc
     5161 cccgtgaacg tcgctctccg ctgcgaccaa tacatgcgcg cctacctttc gtgtttcctg
     5221 cgggtattca ggtccacgaa agacggtaga aagacgctgt ttcgcagcat cgactcccga
     5281 gtaaacggga tacgtcgatc aggtacgctc gacgttccgt caacgcgcga ggaatctgac
     5341 gagaacatga agacgtcgga gactcagacg aggtcgcgcg tcttcgccgg cacccaaatc
     5401 agcgctcacg aagccggcga gcgctcctcg acctcgagga cgaagccctg gcctcgcacg
     5461 gtacggatgc ggatccgcag cggggcaacg cgacggcgca gcaacgagat gcgcgagtcg
     5521 atcgaccgtt ccttgggtgc gcgattcgtg ggccacgcgg cccgcgccag cgtacgccgg
     5581 ccgacgacgt agccctcgcg caagagcagt acggacgcca accgttcctc gatcgggggc
     5641 aacgccaccc attgggtgcc gcggtgcaac acaccgaact cgtcgatgtg gggctcggcc
     5701 ggcgcgacga cgcgcgatgc gagcgtgtgc atgagaggtt cctccacgtc ggcgtcgcgg
     5761 cgtgtgccgc gacaacgtca agcagtgacg tggaggtgag gaggaccgcg ccgaacggcg
     5821 cgcggcggcc cgtgcagacg caggcgcggc gtgagggtcg cgagcgcgcg ctggcgggat
     5881 ccgcgccgca agagtgcggc gaagagcgcg gcggcgaagc cgagcaccat tactgcggtg
     5941 cggaggtcgc gccgccgttg cgtgaccgac gcgacgaccg cgggcgcgga cgtgactcgc
     6001 gccgtgatgg tggcgcggtc gtcctggacg gtctcggcga ccggtcgcat gcggtcgtcg
     6061 accatgcggg cgagcgcgaa gagttgggag accgccgcca agaaggcggc agcaagcagg
     6121 agcagtcgca ccctacgcat cgggagcacc gtaggaccgg cagatgacct acacatgcgc
     6181 cctgcgttac ggcgtggtgc caggttcctg actgctcgct gacggagtgc ggggaccggc
     6241 ggccgggcct gatcgtgcgc ccgtggtgct cggccggcga gcaccacgga cgccccgatg
     6301 atcagtgggc tagctcgcgg gttgggtgag gtcggagacc ttcgtgagcc cgtacttgtc
     6361 ggcagtttcc gtcgtgacgt agaagccgtt gacgtcctgg gccgacgatg gcgctgtcgc
     6421 ggtcaggttg tcggcgctca acttctcgac gagcgcgtcg tacgtctcct ggctgtcggc
     6481 cgtcggcgta ccttcgaggt acgtgagcaa cgtgcccgtg tactcgccgt agaggtcgat
     6541 cgtcccgtcc ttgacgagcg ggtagaccgt ctccgtcggg ccgatgttgt ccttgtacga
     6601 gatgtcgtag cccttggccg cgagcgcttg cccgtaggcc tggctcaaca actgcgcgcc
     6661 ggcgaaatcc ttggagccga ccgtgaggct cgtcccttcg ccggtcgttc cggcttcggt
     6721 caagccgttg cgctcgagga atgcacccgc gacgtccgcg gggtcctcct tgtcgttgaa
     6781 cacggacaac gccatcgtgt tgtactcgtc gagcgtgagc tttccgttca cctcgtcgat
     6841 gatgctcgtg acgtcgtcgg tcgccttgtc cttgttgatg accgccgtcg ggttgtcggc
     6901 gggctggagg cccttgtcgt cttcgagcaa cacgaagtcg tcgtcgatga cactgctgcc
     6961 ggtgaacaac aggccgacgt cgatgtcgcc gtcctgcaac gccttcaccg tgacggggcc
     7021 gccca
//
LOCUS       DASUGA010000287         2080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_142924, whole genome shotgun sequence.
ACCESSION   DASUGA010000287 DASUGA010000000
VERSION     DASUGA010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142924"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VFX21_05675"
     CDS             <1..885
                     /locus_tag="VFX21_05675"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX5095480.1"
                     /translation="LDMGRAAADPARDRLARSSTASAPTTIAQREAPTAVERTAVAAG
                     ARDTTLAARNDSASIAVRVRTVSGRPWASVSGCVHPLGATWYGLVSPTTFVTDGSGYA
                     EVPVAPGRWIVRLSLDPEGYRKLDVAPGAAARLDYVVDPTLAVAGLVVDEARRPLRDV
                     PISVTTLGTDSVRLDAARTDAEGRFRLAIVGNECVVVARAVGYATAFAHVQMPGARPD
                     REARPTIVLQQANTLRGLVANQRGVPVGGCLVTAGSHAAQTDDELPPDAESAVSLADG
                     TFLIERLAAGPHIVRARA"
     gene            892..>2080
                     /locus_tag="VFX21_05680"
     CDS             892..>2080
                     /locus_tag="VFX21_05680"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX5095481.1"
                     /translation="MPWRGSVDVTAGALTTCFIELRSAGTITGRVQSTSGEPVAGALL
                     VGYAGKNVACRTRSDAAGAFVLAPLPPGLALVWAHHKTAGWGRVEHDLGDPGQVVVTL
                     TSGPTAQGRCVDQSGALLPGVDVELRMIDAAGRVFVDAHAAHSGDDGRFRVANVATEA
                     DLWVTAQKAGCRGASGRFAAAADVELVLTTTIDDCILAGRVVDPEGRPVAGATVLVHG
                     SSIGGAYGGATSDDGRFAITDLSRCTMRVTLEHPEFALTTIAQVVSVGGRQELGDLTI
                     VRGGRVRVAVDRPGSAAPSGSVYLLDADGRSASTFELVNGLAVSATIPPGEYQALIAA
                     SHCETCAFRVTVVAGCETTLARTLRPGLPIAVAFELPTTVPTEAPLVGTLTRGGETLY
                     RFTV"
BASE COUNT          298 a          711 c          745 g          326 t
ORIGIN      
        1 ctcgacatgg gccgcgccgc ggcagaccct gcgcgagatc gactcgcacg ttcgtcgact
       61 gccagcgcgc ccacgacgat cgcgcagcgt gaggcgccaa cggccgtcga gcgaacggct
      121 gtcgccgccg gcgcgcgcga cacaacgctc gcagcgcgca acgacagtgc gagcatcgca
      181 gtgcgtgtgc gcacagtcag cggtcgaccg tgggccagcg tgagcggctg cgtgcatccg
      241 cttggcgcca cgtggtacgg cttggtgagc ccgaccacgt tcgtgacaga tggctccggc
      301 tacgccgagg tgccggtcgc cccgggacgc tggatcgtgc gcctttccct cgacccagag
      361 ggttaccgta agctcgacgt ggcaccgggt gccgcagcgc gactcgacta cgtcgtcgac
      421 ccgacgctcg cggtcgccgg cctcgtcgtc gacgaggcac ggcggccgtt gcgcgatgtc
      481 ccgatcagcg tgacgacgct tggtacggac agtgtgcggc tcgacgccgc gcgcaccgat
      541 gccgagggcc gctttcgcct cgcgatcgtc ggtaacgagt gcgtagtcgt cgcccgcgct
      601 gtcggctatg ccactgcgtt cgcgcacgtg cagatgcccg gcgcgcggcc cgatcgtgag
      661 gcgagaccga cgatcgtgct gcagcaggcc aacactctgc gagggctcgt agcgaatcaa
      721 cgcggagtcc ccgtgggggg ctgtctcgtc acggcaggca gccatgccgc acagaccgac
      781 gacgaactcc cgcccgacgc cgagagcgcg gtgagccttg ccgacggcac gttccttatc
      841 gagcgcttgg ccgccgggcc acacattgtg cgcgcgcgcg cctaagacca catgccgtgg
      901 cgtggctccg tcgacgtcac cgcgggggcg ctgacgacgt gcttcatcga gctgcggtct
      961 gccgggacca tcactggccg cgtccaatcg acgagcggtg aaccagtcgc gggcgcgctg
     1021 ctggtcggct acgccgggaa gaacgtagcg tgtcgtaccc gttcggacgc cgctggcgca
     1081 ttcgtgctcg cgccgctgcc accgggtttg gccctggtct gggcccacca caagacggcc
     1141 ggctggggcc gcgtggagca tgacctgggc gacccgggtc aggtggtggt cacgctgacc
     1201 tcggggccga cggcgcaggg acgttgcgtc gaccagagcg gtgccttgct cccaggcgtc
     1261 gatgtggaat tgcgcatgat cgacgccgcc ggccgcgtgt tcgtggacgc gcacgccgcg
     1321 cattccggcg acgacgggcg cttccgcgtc gcgaacgtcg ccaccgaggc cgacctgtgg
     1381 gtcactgcgc agaaggcggg ctgccgcggc gcgtcgggcc ggtttgccgc cgcagccgac
     1441 gtggagctgg tgctgaccac gaccatcgac gactgcattc tggctggccg cgtcgtcgat
     1501 cctgaaggcc gaccggttgc cggtgctacg gtgctggtcc acggtagcag tatcggcggc
     1561 gcctacggag gtgcgacgtc cgacgacggc cgattcgcga tcactgactt gtcccgctgc
     1621 acgatgcgcg tgacgttgga gcacccggag ttcgcgctga caacgattgc gcaggtcgtc
     1681 agcgttggcg gacgccagga gcttggtgac ctgacgatcg ttcgcggcgg acgcgtgcgg
     1741 gttgcggtcg acaggccagg cagcgctgcg ccgagtggca gcgtttactt gctcgacgcg
     1801 gacgggagat cagcgtccac gttcgaacta gtcaacggcc tggcggtgtc ggcgacgatc
     1861 ccgccgggcg agtaccaagc cctgatcgcc gcgtcgcact gcgaaacctg tgccttcaga
     1921 gtcacggtcg tcgcggggtg cgagacgacc ctggcccgga cactgcgacc tgggctgccg
     1981 atcgctgtgg cgttcgaact gccgacgacc gtgccgaccg aggcgccgtt ggttggcacc
     2041 ctgacccgcg gtggcgagac gctctaccgc ttcaccgtcc
//
LOCUS       DASUGA010000288         2287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1696121, whole genome shotgun sequence.
ACCESSION   DASUGA010000288 DASUGA010000000
VERSION     DASUGA010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1696121"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VFX21_05685"
     CDS             complement(<1..493)
                     /locus_tag="VFX21_05685"
                     /inference="COORDINATES: protein motif:HMM:NF018777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HEX5095482.1"
                     /translation="MIVALWLAPGADVDALANVGGRVCIAVDDQPYTRDPVDVLLEVP
                     DDYDVAPLIRLARSMDCIPVETHVQLDGPTSPSVTMVAFVRRAEGLTHEEFVEHWTQR
                     HAPLALKHHIGLVRYVQHVVTDGGAVDGIAELTFASRKDYDTKFYDDETGKAIIREDV
                     KKFI"
     gene            complement(593..1198)
                     /locus_tag="VFX21_05690"
     CDS             complement(593..1198)
                     /locus_tag="VFX21_05690"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5095483.1"
                     /translation="MPRARKAPADVDVRTALLDAAGDLLSAEGPAALTTRRLADAAGT
                     TTQSIYTLFAGKEGVVRAMYREGYERLHARMRRVPHTADPLDDLLKLGRSYRAAALAS
                     PHYYTVMFGHPVPEFVPNADDLAIARVAQQILTDGVQRCVDARVLRAGSDARHVAQWL
                     WATSHGLVSLELLGALDLGPRRGVSATYDEYLRYSLLPFLA"
     gene            1252..1713
                     /gene="smpB"
                     /locus_tag="VFX21_05695"
     CDS             1252..1713
                     /gene="smpB"
                     /locus_tag="VFX21_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX5095484.1"
                     /translation="MSRRGDQVAITNRKARHDYFVLDTYECGIVLKGAEVKSIREGHA
                     NLQEAYARVDDGEVWLHGMHILPYKFSQENLDPVRKRKLLLHRREIERIAVATGEKGI
                     TLVPLRIYFKDGRVKVEIGTARGKHHYDKRQALAARDAKRETERALKGQRD"
     gene            complement(1723..2088)
                     /gene="trxA"
                     /locus_tag="VFX21_05700"
     CDS             complement(1723..2088)
                     /gene="trxA"
                     /locus_tag="VFX21_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003946714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX5095485.1"
                     /translation="MSTVELTMDNFEDTVRENDIVLVDFWASWCGPCRMFAPTFEASS
                     EQHPDIVFGKIDTEAQQDLAGAFGIMSIPTLMIFRDQIGVFSQPGAIPAEALEDLIRQ
                     VRELDMDAVRASLAEQPAS"
BASE COUNT          401 a          757 c          788 g          341 t
ORIGIN      
        1 cgatgaactt cttgacgtct tctctgatga tcgccttgcc cgtctcgtcg tcgtagaact
       61 tcgtgtcgta gtccttgcgc gacgcgaacg tgagctcggc gatgccgtcg accgcaccgc
      121 cgtcggtgac gacgtgttgc acgtagcgga ccagcccgat gtggtgcttc agcgccaacg
      181 gcgcgtgacg ctgcgtccag tgctcgacga actcttcgtg cgtgaggccc tcggcgcgac
      241 gcacgaacgc caccatcgtc acgctcggcg acgtgggacc gtcgagctgc acgtgcgtct
      301 cgaccgggat gcagtccatg ctgcgcgcga gccgaatgag tggcgcgacg tcgtagtcgt
      361 ccgggacttc caggagcacg tcgaccggat cgcgcgtgta gggctggtcg tcgaccgcga
      421 tgcacacgcg tccgccgacg ttcgcgagcg cgtcgacgtc ggcacccggt gcgagccaga
      481 gtgcgacgat cacgagaggg cggtatccgt gacgtcggcc gggtcggcct cacacaccgg
      541 cacgccgagg cggcactcgg ggaaccagtt gtcgtgcggc acgggccgga ggctacgcaa
      601 gaaacggcag caacgagtag cgcaagtact cgtcgtacgt cgccgagaca ccgcgtcgcg
      661 ggccgaggtc gagcgcgccg agcaattcga ggctgacgag gccgtgcgac gtcgcccaca
      721 gccactgcgc gacgtggcgg gcatccgacc cggcgcgcag gacgcgcgcg tcgacgcagc
      781 gttgcacccc gtcggtgagg atctgctgtg cgactcgagc gatcgcgagg tcgtccgcgt
      841 tcggcacgaa ctccggcacc ggatgcccga acatgactgt gtagtagtgc gggctcgcga
      901 gcgcggccgc ccggtacgac cgcccgagct tcagcaagtc gtcgagtggg tcggcggtgt
      961 gcggcacccg gcgcatgcgc gcgtgcaggc gttcgtaacc ctcgcggtac atcgcccgca
     1021 cgacgccttc cttgcccgcg aagagcgtgt agatcgactg cgtcgtcgtt ccggccgcgt
     1081 cggcgaggcg gcgcgtcgtc aaggccgccg ggccctccgc ggacagcagg tcgccggcgg
     1141 cgtccagcag cgcggtgcgc acgtcgacat cggcgggagc cttccgagcg cgcggcatgc
     1201 ccgcagcata acggtgttat gacatgcgat cgctgcggta ccgtcgtcgt gatgtcacgt
     1261 cgcggcgacc aggtcgccat cacgaaccgc aaggcccggc acgactactt cgtgctggac
     1321 acctacgagt gcggcatcgt gctgaagggc gccgaggtca agtcgatccg cgagggccac
     1381 gccaacctgc aagaggcgta cgcccgcgtc gacgacggag aagtctggct gcacggcatg
     1441 cacatcctcc cctacaagtt ctcgcaggag aacctcgacc cggtccgcaa gcgcaagctg
     1501 ctcctccacc ggcgcgagat cgaacggatc gcggtggcga ccggcgagaa gggcatcacc
     1561 ctcgtgccgc tgcgcatcta cttcaaggac ggtcgcgtca aggtcgagat cggcaccgcg
     1621 cgcggcaagc accactacga caagcggcaa gcattggccg cgcgcgacgc gaaacgagaa
     1681 acggaacgcg ccctcaaggg ccagcgcgac tgacccgcgc ggtcagctcg cgggctgctc
     1741 ggccagcgac gcgcgcacgg cgtccatgtc gagctcgcgc acctggcgga tcaagtcttc
     1801 gagcgcctcg gccgggatcg cgccgggctg cgagaagacg ccgatctgat cgcggaagat
     1861 catcagcgtc gggatcgaca tgatgccgaa cgcaccggcg aggtcctgct gcgcttcggt
     1921 gtcgatcttt ccgaacacga tgtcggggtg ctgctcggac gacgcctcga acgtcggggc
     1981 gaacatccgg cacggcccgc accaggacgc ccagaagtcg acgaggacga tgtcgttctc
     2041 gcgaacggtg tcctcgaagt tgtccatggt gagttcgacg gtcgacatgc aagagctcca
     2101 gtaggtgtcg ggaagtcaga gatacaaccc tggtatcggc cggatcattc ccgggccgga
     2161 agggcccgga tgttcacccg gatcggccgg tcgtagcgca ggtagtacca ggaccagcgg
     2221 gccaagacga gggcccggtt ctcgaagccg atcaagtagt agaggtgcac gatcagccag
     2281 acgaccc
//
LOCUS       DASUGA010000289         4980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1400926, whole genome shotgun sequence.
ACCESSION   DASUGA010000289 DASUGA010000000
VERSION     DASUGA010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400926"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1335
                     /locus_tag="VFX21_05705"
     CDS             151..1335
                     /locus_tag="VFX21_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HEX5095486.1"
                     /translation="MDLIVSGAGPAGTAAAIAAARLGLEVVCFDKAAVGRDKTCGDGL
                     TTQALRLLEQLGLTRRALAAAGYVSVHETVIVSPSGRRAVLPLPTDGEHAGVVPRAGL
                     DAALVARAREGGVDVREQCAVDDVVVSSDGVKVRTETGDTLEARALVAADGHWSPVRR
                     RLHPDAPRDLGTWHAARQYFDHVDDDRLWVIFERDLLPGYAWVFPLPGGGANVGYGVL
                     RDQERAGRDLKALWPDLLARPLVREILGPRAVAREPVRAWPIPTHFDPARLADGRVLY
                     AGDAAAVVDPLTGEGIAQALETGMLAADAIARGGAADELAARYRRSVQRSLGRDLRFA
                     SLLQRVLTTERGARAAIKAADLSPWTRRSFARWLFEGYPRALLLTPDRWHRGMFGASG
                     AY"
     gene            1590..1976
                     /locus_tag="VFX21_05710"
     CDS             1590..1976
                     /locus_tag="VFX21_05710"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5095487.1"
                     /translation="MAGGIAEIKIDKSPDEVWKVVGDFGGLANWMPGIESCELDGDVR
                     KLAMMGIEIHEQLRELSEDDHRIAYGIVQSPMPLERHHATIAVAADGDGSVVTWEVEV
                     LPDEMLPAFLPIYEQSLGQLKSHLEG"
     gene            complement(1980..2405)
                     /locus_tag="VFX21_05715"
     CDS             complement(1980..2405)
                     /locus_tag="VFX21_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5095488.1"
                     /translation="MPRGRGVADHLADVRLFETCSARDRQTLAKHIEVVDFPAGKRIV
                     SQGDAGNAFYVMLDGHAVVRRNGRKVGELHAGDYFGELALLDPSPRNADIVANSDVTV
                     GRLLVGAFRQMLRDVPAMNERLLAGLARRVRDADRHAVE"
     gene            complement(2463..3125)
                     /locus_tag="VFX21_05720"
     CDS             complement(2463..3125)
                     /locus_tag="VFX21_05720"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5095489.1"
                     /translation="MRFAVTLRRALPLGAVASLFVFVALAIDVSDDRGWIADVDRDFA
                     ELAHDGVDDRPPAIEASKTGTRLGDTMTLFVVVSLLTVAALARGRARLAVIVVVTAIS
                     GALLNHAVKELVDRARPQFADPVAVGHGPSFPSGHAMNSMIAYGSVVLVVWILHRRFA
                     MIAAVVSAIVVAVVGVTRVTLGVHYVSDVVGGWLLGAVWLALAVVVLGDCRTRQFARA
                     TE"
     gene            complement(3129..4190)
                     /locus_tag="VFX21_05725"
     CDS             complement(3129..4190)
                     /locus_tag="VFX21_05725"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5095490.1"
                     /translation="MSEREGKSAPERAGEAAQALYGAVGIGVPGYRRSDVASLAGIDR
                     SRSVKWWRAMGFPEVPEDVPAFSELDVEILRRLAAVTGAGLVDDENIMRLARVLGASF
                     SRIADAQVAVLEQLVASLPGADPDASTRDGIEALLTAPGESVFDLLEDSLVYVWRRHL
                     LAALGRRLEAADEGATERAVGFADLSGFTRLSQRMPAHRLAQVVDRFEEIAFDVISDR
                     GGRAVKFIGDEAMFVADTLPDAVDIALDLAERLRAVRDMPVIHCGIACGRTVTVGGDV
                     FGPAVNLAARLTTIARPDTIVIPRDAVGQLAERDDIEIVRVQRSFELKGIGDTRVVAV
                     RRKRARREATPRPIRALEH"
     gene            complement(4287..>4980)
                     /locus_tag="VFX21_05730"
     CDS             complement(4287..>4980)
                     /locus_tag="VFX21_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5095491.1"
                     /translation="YAELMGLTAFMCMKVPNGLDHRRAALTEPMAVGRHAVARSGVTR
                     GESALVLGAGPVGLAVVAELQRSGIETIVASDYSPRRRSVARAMGATATVDPAHESPI
                     EVWQREDGRRPLVVFDAVGVPGTLMQALRAAPPLARVCVVGSCMEPDTVMPLIPQAKQ
                     LTVIFSFAYDPFEFGDTLRAIAEGEIDVTPMLTGTCGIDGVPAAFDALGRPEEHVKIL
                     VEPGGPAMPAPL"
BASE COUNT          773 a         1781 c         1702 g          724 t
ORIGIN      
        1 gttccgctcg aacgtcgaac cgtatgtctt tgtcgctccg gtcgtgctcg cgtgaggcag
       61 cccggattgt tggcccgccc tccgctccgg ccagtgcctc cgcaagctcc ggcacggcgc
      121 cgtgcggcga cgggccggta ccgtcgccgt gtggatctga tcgtcagcgg ggccggaccg
      181 gccgggacgg ccgccgcgat cgcggccgcc cgcctcggcc tcgaggtcgt ctgcttcgac
      241 aaggccgccg tcggccggga caagacctgt ggcgacgggt tgacgacaca agccctccgc
      301 ctcctcgaac agctgggcct gacgcgacgc gcgctcgccg ccgccggcta cgtctcggtg
      361 cacgagaccg tgatcgtctc ccccagcggc cgccgcgcgg tcctgccgct gccgaccgac
      421 ggcgaacacg ccggcgtcgt gccacgcgcc ggactggacg cggcgctcgt cgcgcgagcg
      481 cgggagggcg gcgtcgatgt ccgcgagcaa tgcgcggtcg acgacgtcgt cgtcagcagc
      541 gacggcgtga aggtgcgcac cgagacgggc gacacgctgg aggcacgcgc gctggtcgcc
      601 gccgacggcc actggtcacc ggtgcggcgg cgcctccatc ctgatgcgcc gcgcgatctc
      661 ggcacctggc acgcggcgcg ccagtacttc gaccacgtcg acgacgaccg cctgtgggtg
      721 atcttcgagc gtgatctgct gcccgggtac gcgtgggtgt tcccgctgcc cggcggcggc
      781 gcgaacgtcg gctacggcgt gctgcgcgat caagagcgcg cgggacgcga cctcaaggcg
      841 ttgtggcccg acctgttggc gcgcccgctc gtgcgcgaga tcctcggacc gcgcgccgtc
      901 gcccgcgaac cggtacgcgc gtggccgatc cccacgcact tcgatccggc gcgcctggcc
      961 gacggccgcg tgctgtacgc cggcgacgcg gccgccgtcg tcgacccgct gaccggtgaa
     1021 ggcatcgcgc aggcgctcga gacgggcatg ctcgccgctg acgcgatcgc gcggggcggt
     1081 gccgcggacg agctcgccgc gcgatatcgc cgctcggtgc aacgctcgct cgggcgcgac
     1141 ctgaggttcg cgtcgttgct ccaacgcgtg ttgacaaccg aacgcggcgc ccgcgccgcg
     1201 atcaaggctg ccgacctctc cccctggacg cgccgcagct tcgcccgctg gctgttcgag
     1261 ggctatcccc gcgctctcct cctcaccccc gaccgctggc accgcgggat gttcggcgcc
     1321 tccggcgcat actgactttg tcgctctcga cctgctcgcg tgagacagcc cggattgttg
     1381 gccggcctcg agactccggc cagtgcctcc gcaagctccg gcacgatctt tctttgtcgc
     1441 cttcgacgtg ctcgcgtgag gcggccggga ttgtcggccc gcctcaggac tccggccagt
     1501 gcctccgcaa gctccggcac ggcgccgtac gacgcgcgcg gtccgcccat ggcatgctgc
     1561 gcgctccgaa cgaggggaag tgacacgaca tggccggcgg cattgctgag atcaagatcg
     1621 acaagagccc cgacgaggtg tggaaggtcg tcggcgactt cggcggcctc gccaactgga
     1681 tgccgggcat cgagtcgtgc gagctcgacg gcgacgtgcg caagctcgcg atgatgggca
     1741 tcgagatcca cgagcagctt cgcgagctgt ccgaggacga ccaccgcatc gcctacggca
     1801 tcgtccagtc gccgatgccg ctcgagcggc accacgccac gatcgcggtg gccgccgacg
     1861 gcgacggatc ggtcgtgacg tgggaggtcg aagtgctccc cgacgagatg cttccggcct
     1921 tcctacccat ctacgagcag agcctgggcc agctgaaatc acatctcgag ggctaggagc
     1981 tactccaccg cgtgccggtc ggcgtcgcgc acacggcgcg ccaggcccgc gagaagccgt
     2041 tcgttcatcg cggggacgtc gcgcaacatc tgccggaacg cgccgacgag gagccgcccg
     2101 acggtgacgt cggagttggc gacgatgtcg gcgttgcgcg ggctcgggtc gagcagcgcg
     2161 agctcaccga agtagtcgcc cgcgtgcagc tcgccgacct tgcggccgtt gcggcgcacg
     2221 accgcatgcc cgtcgagcat cacatagaac gcgttgcccg cgtcgccctg gctgacgatc
     2281 cgcttgccgg ccgggaagtc gacgacctcg atgtgcttgg ccagcgtctg ccggtctcgc
     2341 gccgagcacg tctcgaacaa ccggacgtcg gcgagatggt cggcaacgcc gcgaccgcgt
     2401 ggcatgggcc gactgtagag gtttggcaaa ggaccgcgtg ggacaccggc cattggacgt
     2461 cgtcattccg tcgcgcgagc gaattgccgc gtccggcaat caccgagaac cacgacggca
     2521 agcgcgagcc agacggcgcc gaggagccag ccacccacga cgtccgacac gtagtgcact
     2581 cccagcgtca ctcgcgtgac gccgacgacc gcgaccacga tggcgctcac aacggccgcg
     2641 atcatcgcga agcgtcggtg aaggatccac acgacgagca cgacgctgcc ataggcgatc
     2701 atcgagttca tggcatgccc agaaggaaac gagggcccat gaccgaccgc gaccgggtcg
     2761 gcgaactgcg gccgggcgcg gtcgacgagt tccttcaccg cgtgattgag cagtgcgccc
     2821 gagatcgccg tcacgacgac gatcacggcg agcctcgccc ttccccgcgc gagcgcggcg
     2881 accgtgagca ggctcacgac gacgaacagc gtcatcgtgt cgcccagcct cgtgcccgtc
     2941 ttggacgctt cgatcgcggg cggacggtcg tcgacaccgt cgtgagcaag ttcggcgaag
     3001 tctcgatcga cgtcggcgat ccatcctctg tcgtccgaca cgtcgatcgc cagcgcgacg
     3061 aagacgaaga gagatgcaac cgcgccgagc ggtagcgctc gtcgaagtgt gacggcgaag
     3121 cgcacagatc agtgctccaa cgcacggatg ggccgcggcg tcgcctctcg tcgcgcgcgc
     3181 ttgcgacgga cggcgacgac gcgggtgtcg ccgattccct tgagctcgaa gctgcgttgg
     3241 acgcgaacga tctcgatgtc gtcgcgctcg gcgagttgtc cgaccgcgtc gcgcgggatc
     3301 acgatcgtgt ccggacgcgc gatcgttgtg aggcgggcgg cgagattgac ggcgggcccg
     3361 aacacgtcac cgccgacggt gaccgtgcga ccgcacgcga tgccacagtg gatcacgggc
     3421 atatcgcgga ccgcacgcag ccgctcggca agatcgagcg cgatgtcgac cgcgtccggc
     3481 aaggtgtcgg caacgaacat cgcctcgtcg ccgatgaact tgacggcccg tccgccgcga
     3541 tccgagatga cgtcgaaggc aatttcctcg aaccggtcga cgacctgtgc cagccggtgc
     3601 gcgggcatcc gttgactcag cctggtgaag ccactcagat cggcgaagcc gactgcacgt
     3661 tcggtcgcgc cttcatcggc cgcctccaga cgccggccca gcgccgcgag caagtgtcgt
     3721 cgccacacgt agaccagcga gtcttccaac agatcgaaga cggattcgcc gggcgccgtc
     3781 aggagcgcct cgatcccatc tcgagtactc gcgtcggggt cggcaccggg cagtgacgcg
     3841 acgagctgct cgagcactgc aacctgcgca tcggcgatgc gactgaacga cgcacccagc
     3901 actcgcgcga ggcgcatgat gttctcgtcg tcgacgagcc cggcaccggt gaccgccgcc
     3961 agacgtcgaa ggatctcgac atcgagctcg gagaacgcag gcacgtcctc cggcacctcc
     4021 ggaaagccca tcgctcgcca ccacttcacc gaccggctcc ggtcgattcc tgcaaggctc
     4081 gctacgtcgg agcgtcggta tccggggacc ccgatgccga cggctccata cagcgcctgc
     4141 gccgcttcac cggcgcgctc gggtgcgctc ttgccctcgc gctcgctcat cgttccatcg
     4201 ttacagacga gcggcgcgtc ggcatcgagc cggtccggac gctcgtgacc gatccgtgct
     4261 tgcgaacgat ctcggcgtgc gacgcgctac aggggtgcgg gcatcgccgg gccgcccggt
     4321 tcgacgagga tcttcacgtg ctcctcgggc cgcccgagag cgtcgaacgc ggccggcaca
     4381 ccgtcgatgc cgcaggtgcc ggtcagcatc ggcgtgacgt cgatctcgcc ttcggcaatg
     4441 gcccgcagcg tgtcgccgaa ctcgaacggg tcgtacgcga acgagaagat gaccgtgagc
     4501 tgcttggcct gtgggatcaa cggcatcacg gtgtcgggct ccatgcacga gccgacgacg
     4561 cacacccgcg cgagcggcgg cgccgcgcgc agggcttgca tcaaggtgcc gggaacgccg
     4621 acggcgtcga acacgacgag cggccgccgg ccgtcttcgc gttgccacac ctcgatcggt
     4681 gactcgtgcg cgggatcgac ggtcgccgtc gcgcccatgg cgcgcgcgac actgcggcgg
     4741 cgcggtgagt agtcgctcgc gacgatcgtt tcgatgcccg accgttgcag ctcggcgacg
     4801 accgcgagac cgaccgggcc cgcaccgagg accaacgccg actcgccgcg cgtcacgccg
     4861 gagcgggcaa ccgcgtggcg gccgaccgcc atcggctcgg tcaacgccgc gcgccggtgg
     4921 tcgagaccgt tcggcacctt catgcacatg aacgcggtga gccccatcag ctcggcgtag
//
LOCUS       DASUGA010000290         2196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1032451, whole genome shotgun sequence.
ACCESSION   DASUGA010000290 DASUGA010000000
VERSION     DASUGA010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032451"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VFX21_05735"
     CDS             <1..468
                     /locus_tag="VFX21_05735"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HEX5095492.1"
                     /translation="LEQLGKHGGFDLTIEALGDLDVDLHHTVEDVGITLGVALREALG
                     DKAGVRRFANSLVPLDEALVQVAVDLSGRPFLVYDVDPISEWIGTFDPQLAEEFWKGF
                     VDGARLTLHLRSVSGKNGHHIIEASFKGVARALRDAVRIEGTGVPSTKGTLSS"
     gene            465..1067
                     /gene="hisH"
                     /locus_tag="VFX21_05740"
     CDS             465..1067
                     /gene="hisH"
                     /locus_tag="VFX21_05740"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009645214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX5095493.1"
                     /translation="MIAVIDYGIGNLRSAEKALAHVGADARLTSDVAEIAKASAVVLP
                     GVGAFGACMRALRASELEAVTKEAATDGRPFLGICIGMQMLFDGSDESPDVAGLGIVG
                     GRVTSLPTSVRLPQMGWNTLDITPDSLLCAGLGGDDWLYFVHSFAPEPTDLSIVAAWC
                     DYGRRFAAAIEAGPVWATQFHPEKSGAVGLQLLRNFVEAA"
     gene            1067..1804
                     /gene="hisA"
                     /locus_tag="VFX21_05745"
     CDS             1067..1804
                     /gene="hisA"
                     /locus_tag="VFX21_05745"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX5095494.1"
                     /translation="MELYPSIDLREGRVVRLLQGDFDRETHYSDDPVAVARTFADAGT
                     PWIHVVDLDAARGGEARNLQHLEAICAAVDVKVQASGGVRTVADASARFASGATRVVV
                     GSAAVEHPEVVDELAALHPGQVAVGLDARGRDVAIHGWETSTGLDLLDLVARFDDPRI
                     GALVFTEIGRDGTLHGPAFDLLTSVMETTSVPVIASGGVSELDDIRALAGLRGGGRRL
                     AGVITGKAIYEGRFSVAEGVAACSASV"
     gene            1786..>2196
                     /locus_tag="VFX21_05750"
     CDS             1786..>2196
                     /locus_tag="VFX21_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEX5095495.1"
                     /translation="MLGVRVIPCLDVDAGRVVKGVNFVGIRDAGDPVELAARYDAEGA
                     DELVFLDITASSDARDTTVHMVECVADQVFIPFTVGGGVRTVEDVRRMLRAGADKVSL
                     NTAAVAAPELVAQGAEEFGAQCVVCAIDAKHRPGQ"
BASE COUNT          311 a          761 c          781 g          343 t
ORIGIN      
        1 ctcgagcagc tcggcaagca cggcgggttc gacctgacga tcgaggcgtt gggtgatctc
       61 gacgtcgacc tccaccacac cgtcgaagac gtcggcatca cgctcggcgt cgcgctgcgc
      121 gaggccctgg gcgacaaggc cggggtgcgg cggttcgcga actcgctggt gccgctcgac
      181 gaagcactgg tgcaagtcgc cgtcgacctt tccgggcggc cgttcctcgt ctacgacgtc
      241 gacccgatct ccgaatggat cggcaccttc gacccgcagc tcgccgagga attctggaag
      301 ggcttcgtcg acggcgcgcg cctcacgctc cacttgcgca gcgtgtcggg caagaacggg
      361 caccacatca tcgaggcgtc gttcaagggt gtcgcccgcg cgttgcgcga cgcggtccgc
      421 atcgagggga ccggcgtgcc gtccacgaag ggcacgttgt cgtcttgatt gccgtcatcg
      481 attacgggat cgggaacctg cgatccgcgg agaaagcgct cgctcacgtc ggcgccgacg
      541 cgcgcctgac gtcggatgtc gccgagattg cgaaggcgag cgcggtcgtc ttgcccggtg
      601 tcggcgcgtt cggcgcgtgc atgcgggcgt tgcgagcgag cgagctcgaa gcggtgacga
      661 aggaggccgc caccgacggc cgaccgttcc tcgggatctg catcggcatg cagatgctct
      721 tcgacggcag cgacgagtcg cccgacgtcg ccggcctcgg catcgtcggc ggtcgcgtga
      781 cgtcgttgcc cacgtcggtg cggctgccgc agatgggctg gaacacgctc gacatcaccc
      841 ccgacagctt gctgtgcgcg gggctgggcg gcgacgactg gctgtacttc gtgcactcgt
      901 tcgcgcccga gcccactgac ctctcgatcg tcgccgcgtg gtgcgactac ggccgtcgtt
      961 tcgccgccgc gatcgaagcc gggcccgtgt gggcaacgca gttccacccc gagaagagcg
     1021 gcgcggtcgg tctccagctg ctgcgcaact tcgtcgaggc cgcgtgatgg agctctaccc
     1081 gtcgatcgac ttgcgcgagg gccgcgtcgt ccgcctgttg caaggcgact tcgatcgcga
     1141 gacgcactac agcgacgacc ccgtggcggt ggcgcgtacg ttcgcggacg caggcacgcc
     1201 gtggatccac gtcgtcgacc tcgatgccgc acgcggcggc gaagcgcgca acctgcagca
     1261 cctcgaagcg atctgcgccg cggtcgacgt caaggtgcag gcgagcggtg gtgtgcgtac
     1321 ggtcgcggac gcgagtgcgc ggttcgcgtc gggcgcaacg cgtgtcgtcg tcggcagcgc
     1381 cgccgtcgag caccccgaag tcgtcgacga actggccgcg ttgcacccgg gccaggtcgc
     1441 ggtcgggctc gacgcccgcg gccgcgacgt cgcgatccac ggctgggaga cgagcacggg
     1501 gctcgacctg ctcgacctcg tcgcccgctt cgacgacccg cgcatcggcg cgctggtgtt
     1561 caccgagatc ggccgcgacg gcaccctgca cggacccgcg ttcgacctcc tcaccagcgt
     1621 gatggagacg acgagcgtgc cggtcatcgc gagcggcggc gtctccgagc tcgacgacat
     1681 ccgtgccctc gccggcctcc ggggcggcgg ccggcggctg gccggcgtca tcaccggcaa
     1741 ggcgatctac gaaggccgct tctcggtcgc ggagggggtg gcggcgtgct cggcgtccgt
     1801 gtgatcccgt gcctcgacgt cgacgcaggc cgcgtcgtga aaggtgtgaa cttcgtcggc
     1861 atccgcgacg ccggtgaccc cgtcgagctc gccgcgcgct acgacgccga aggcgcggac
     1921 gagctcgtgt tcctcgacat caccgcgtcg tccgatgcgc gtgacaccac tgtgcacatg
     1981 gtcgagtgcg tcgccgacca ggtgttcatc cccttcaccg tcggcggggg cgtccgcacg
     2041 gtcgaagacg tccgccgcat gctgcgcgcg ggcgccgaca aggtgtcgct caacaccgca
     2101 gcagtggccg cgccggaact ggtcgcgcaa ggtgccgaag agttcggcgc ccagtgcgtc
     2161 gtgtgtgcca tcgacgccaa gcaccgtccg ggtcag
//
LOCUS       DASUGA010000291         6327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_306565, whole genome shotgun sequence.
ACCESSION   DASUGA010000291 DASUGA010000000
VERSION     DASUGA010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6327
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306565"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="VFX21_05755"
     CDS             complement(<1..907)
                     /locus_tag="VFX21_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX5095496.1"
                     /translation="MTDVTGSAAEERKARMAKVDALRERGVDPYPVAFERSHSLGDVR
                     AQWDAKLEDGASTDDEVRVAGRVLLMRDQGRLIFVTLRDGTGALQLFVSEGDLGKETF
                     AEFRDGVDLGDWIGVDGTVMKTKKGELSVRIKSARMLSKSLRPLPDKWHGLTDVDARY
                     RQRYVDLIVNEDARRVFEIRFAAMNAIRSELIERGFVEVETPVLHPIPGGATARPFIT
                     HHNALDMELYLRIAPELYLKRLIVGGFDRVFEFARVFRNEGLSTRHNPEFTMLELYQA
                     YADYRDFMDLVERLFQGLADTVLGTR"
     gene            complement(904..1668)
                     /locus_tag="VFX21_05760"
     CDS             complement(904..1668)
                     /locus_tag="VFX21_05760"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX5095497.1"
                     /translation="MLLAIDVGNTQTLVGLFNGHELADHWRIATTAERTSDELALMIQ
                     QFLGFHGFSFDDSITGVAISSVVPRVTAELRQMTERYFAMPALVVEPGVRTGMPILYD
                     NPKEVGADRIANAVGAYDLYGGPSIVVDFGTATTVEAISDRGEYLGGAIFPGVDISLD
                     ALFERAAGLRRVELALPKHVIGKSTQESLQSGAIYGFSAQVDGLVERFMAEVGESTVI
                     ATGGLAVVIIPHSRTVQHYEPWLTLYGLRIIFERNQ"
     gene            complement(1695..2537)
                     /gene="nadC"
                     /locus_tag="VFX21_05765"
     CDS             complement(1695..2537)
                     /gene="nadC"
                     /locus_tag="VFX21_05765"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX5095498.1"
                     /translation="MSTSFDPPVPILRRLVEEALAEDMGVLGDITSIGCINEDQTAVA
                     MFVAREEGVFAGTAAATEVFRQVDWSVTIDWQVRDGMSIEAGMELGQVHGTLRSILAG
                     ERVALNFLCHLSGIASLTRRYVRAAHGKARVRDTRKTLPGLRAVQKAAVRAGGGFNHR
                     DSLSDAVLVKDNHLAALGLTRVVERERARWPGRIVEVECDSLDQVKEAKDAGVDVVLL
                     DNMSPEDVQAAVEVLDGTTKVEVSGRVTLESIGAYANAGVDFIAVGALTHSARSLDIG
                     LDLP"
     gene            complement(2542..4161)
                     /gene="nadB"
                     /locus_tag="VFX21_05770"
     CDS             complement(2542..4161)
                     /gene="nadB"
                     /locus_tag="VFX21_05770"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEX5095499.1"
                     /translation="MFDVLVLGSGIAGLTTALQAARRGLSVLVLTKGELSHSATRYAQ
                     GGVAAALAPPDSPDLHLADTLVAGAGLCDADAVRVLVTEGPDRVRELAALGAQFDAEV
                     DDDGPQLLLAREGGHSLARVVHAGGDATGAEIERALVAAVERGDIEVREGWFAIELLV
                     EKGHCEGVLALNPTGGVEFVRATDTVLATGGAGQCFAVTTNPTLSTGDGIALALKAGV
                     ACADLEFVQFHPTALHHPSMPRPLLSEALRGEGAVLRDDDGVAFMTGVHPLADLAPRD
                     VVSRAIAKRMRESGADHVYLDATMIPEFETRFPTIWRACQSAELDPREDWLPVAPAAH
                     YLSGGVVTDLDGATTLPQLWACGEVACSGVHGANRLASNSLLDGLVYGRRVVEAIAAG
                     RQEAQATGAMTGVLELGVGAADEDVVVLPKATPTEPEKLRGAVQRTMSADCGVVRAAD
                     GLEMASETLTDLNGLAIDLPARTVATYEVLNVLRVSRGIVAAAAAREESRGAHTRSDY
                     PQTSDEFVGRFVLRGARAPQFVPLPAYALEK"
     gene            complement(4187..4588)
                     /gene="panD"
                     /locus_tag="VFX21_05775"
     CDS             complement(4187..4588)
                     /gene="panD"
                     /locus_tag="VFX21_05775"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEX5095500.1"
                     /translation="MNRFMMKSKIHRVTVTGACVDYVGSITLDPELMRLADIREFEQV
                     HVLDIDNGARFETYAMAGGPGDVILNGAAARLVAPGDKVIVITYSSYDAAELERYEPQ
                     IVHVDERNRPIPEAEAVARAGSNVVWIGANS"
     gene            complement(4585..5472)
                     /gene="panC"
                     /locus_tag="VFX21_05780"
     CDS             complement(4585..5472)
                     /gene="panC"
                     /locus_tag="VFX21_05780"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX5095501.1"
                     /translation="MRQLATIADLRAACDDARARGERVGLVPTMGFLHEGHRSLIRAA
                     RAATDFVVVTIFVNPLQFGPTEDLEAYPRDLERDLAACAAEGADAVFTPSVAEMYPRG
                     AVTTVHVDGLTADLCGASRPTHFDGVTTVVTKLFSIVGPCDAYFGRKDAQQLAVVRRM
                     ALDLNLPAAVHGCPLVREADGLALSSRNAYLTADEHAAAPVLFRALRAAADAVAAGER
                     EPEQLRAIVQSTVATEPLVALEYIEVRDAHDLSRVDTLDGDVLVALAARLGRTRLIDN
                     VQLTVSGDDVAADLGEVKQ"
     gene            complement(5553..>6327)
                     /locus_tag="VFX21_05785"
     CDS             complement(5553..>6327)
                     /locus_tag="VFX21_05785"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEX5095502.1"
                     /translation="AVAGRAPDAASTATAAVCLAAQPTLVSGAGQGAQLVIIATPDRA
                     IEGASLTVADSLEPDALVLHLAGSKGIDVFDALREKRPDVRVGALHPLQSFPSTSVGV
                     ERLAGSWAAIAGDAEVEQLAMSLGLRPFAVADADRVRYHAAAVVASNHLVALLGQVER
                     LAAAAGVPFAAFGPLAEASVDNAFRLGPANALTGPVERGDLTTVEGHLAALDSAERDS
                     YRSLAREAARLTGRRDTAMGRLLGDVLAPRAGEPPTDGN"
BASE COUNT         1013 a         2183 c         2213 g          918 t
ORIGIN      
        1 gccgcgtccc cagcacggtg tcggcgagtc cctggaacag ccgctcgacg aggtccatga
       61 aatcgcggta gtccgcgtac gcctggtaca gctcgagcat cgtgaactcg gggttgtgcc
      121 gggtcgacag cccttcgttg cggaagacgc gcgcgaactc gaagacacgg tcgaagccgc
      181 cgacgatgag gcgcttcagg tacagctcgg gcgcgatccg cagatacagc tccatgtcga
      241 gcgcgttgtg atgcgtgatg aacggtcgtg ccgtcgcacc gccggggatg gggtggagca
      301 ccggcgtctc gacctcgacg aagccgcgtt cgatcaactc ggagcggatc gcgttcatcg
      361 ccgcgaaccg gatctcgaac acgcgacggg cgtcttcgtt gacgatcaag tcgacgtagc
      421 gctggcgata gcgggcgtcg acgtcggtga gaccgtgcca cttgtcgggg agcggccgca
      481 gcgacttcga cagcatgcgc gccgacttga tgcgcaccga cagctcgccc ttcttcgtct
      541 tcatgacggt gccgtcgaca ccgatccagt cgccgaggtc gacgccgtca cggaactcgg
      601 cgaatgtctc cttgccgaga tcaccttcgc tcacgaacag ctgcagcgcg ccggtgccgt
      661 cgcgcagcgt cacgaagatc aatcgaccct ggtcgcgcat caacaacacg cgaccggcga
      721 cgcgcacttc gtcgtcggtg gacgcaccgt cttcgagctt ggcgtcccac tgggcgcgca
      781 cgtcgccgag tgagtgcgac cgctcgaacg cgacgggata cggatcgacg ccgcgctcac
      841 gcagcgcgtc gaccttcgcc atccgcgcct tgcgctcttc ggcggcggaa ccggtgacgt
      901 cagtcattga tttcgctcga agatgatgcg caggccgtac agcgtgagcc acggctcgta
      961 gtgctgcacg gtgcgcgagt gcgggatgat gacgacggcg aggccgccgg tcgcgatgac
     1021 cgtgctctca cccacctcgg ccatgaagcg ctcgacgagc ccgtcgacct gggcgctgaa
     1081 cccgtagatc gcgcccgact gcaacgactc ctgcgtggac ttgccgatga cgtgcttcgg
     1141 cagcgcgagc tcgacgcgcc gcaggcccgc ggcgcgctcg aacaatgcgt cgagcgagat
     1201 gtcgacgccg gggaagatcg cgccgccgag gtactcgccg cggtcgctga tcgcctcgac
     1261 cgttgtcgcg gtcccgaagt cgacgacgat cgacggaccg ccgtagaggt cgtacgcgcc
     1321 gactgcgttc gcgatccggt ctgcgccgac ctccttcggg ttgtcgtaga ggatcggcat
     1381 tccagtacgg acgccgggct cgacgacgag ggccggcatc gcgaagtagc gctcggtcat
     1441 ctggcgcagc tctgccgtca cccgcggcac taccgacgag atcgcgacac cggtgatcga
     1501 gtcgtcgaag gagaacccgt ggaacccgag gaactgctgg atcatcaacg ccagctcgtc
     1561 ggaggtgcgc tcggccgtcg tcgcgatgcg ccagtggtcc gcgagctcgt gcccgttgaa
     1621 cagcccgacg agcgtttgcg tgtttccgac gtcgatcgcc aacagcatcg aacgggaatg
     1681 ctaggagacg gggcctaagg caggtcgaga ccgatgtcga gcgatcgggc cgagtgcgtc
     1741 agtgcgccga cggcgatgaa gtcgacaccg gcattggcgt acgcgccgat cgattcgagg
     1801 gtcacgcggc ccgacacctc gaccttcgtg gtgccgtcga gcacctcgac cgccgcctgc
     1861 acgtcctcgg ggctcatgtt gtcgagcaag acgacgtcga cgccggcgtc cttggcttcc
     1921 ttcacctggt cgagcgagtc gcactcgacc tcgacgatgc gcccgggcca gcgggcccgt
     1981 tcgcgctcca cgacgcgtgt gaggccgagc gcggcgaggt ggttgtcctt gacgagcacc
     2041 gcgtcgctca gcgaatcgcg gtggttgaac ccgccaccgg cgcgcacagc ggccttctgc
     2101 accgcacgca ggccgggcaa cgtcttgcga gtgtcgcgga cgcgcgcctt gccgtgcgcg
     2161 gcgcgtacgt agcggcgggt caacgacgcg atgccggaga ggtggcacaa gaagttcagc
     2221 gcgacgcgtt cacccgcgag gatcgagcgc agcgtgccgt gcacttgacc gagctccatg
     2281 ccggcttcga tcgacatacc gtcgcggacc tgccagtcga tggtgaccga ccagtcgacc
     2341 tggcggaaca cttccgtcgc ggccgccgtg ccggcaaaga caccctcttc gcgcgcgacg
     2401 aacatcgcga cggcggtctg gtcttcgttg atgcagccga tcgacgtgat gtcgccgagc
     2461 acacccatgt cctcggcgag cgcttcttcg acgaggcggc gcaggatcgg gaccggagga
     2521 tcgaaagaag tactcatggc ttcacttctc cagggcgtac gccggaagcg ggacgaactg
     2581 cggcgcacgc gcgccccgga gcacgaaacg ccccacgaac tcgtcgctcg tctgcggata
     2641 gtcgctccgt gtgtgcgcgc cacgcgactc ttcacgcgcg gcggcggccg caacgatgcc
     2701 gcgcgacacg cggaggacgt tgaggacctc gtacgtcgca accgtgcgcg ccggcagatc
     2761 gatggcgagg ccgttcaggt cggtgagcgt ttcgctcgcc atctcgagac cgtcggccgc
     2821 gcgcacgacg ccacagtccg cggacatcgt gcgctgcacg gcgccccgca gtttctccgg
     2881 ctcggtcggc gtcgccttgg gcaggaccac gacgtcctca tcggcagcac cgacaccgag
     2941 ctccagcaca cccgtcattg cgcccgtggc ctgggcttct tggcggcccg cggcgatcgc
     3001 ctcgaccacc cggcgcccgt agacgagccc gtcgagcaac gagttcgacg ccaggcggtt
     3061 ggcgccgtgc acgccgctgc acgccacctc gccgcacgcc cacagctgcg gcaacgtcgt
     3121 ggcgccgtcg agatcggtga cgacaccacc cgagagatag tgcgcggcgg gcgcgaccgg
     3181 caaccagtct tcacgcggat cgagctccgc cgactggcac gcgcgccaga tcgtcgggaa
     3241 gcgcgtctcg aactcgggaa tcatcgtcgc gtcgagatac acgtggtcgg cgcccgattc
     3301 gcgcatgcgc ttggcgatcg cgcgcgagac gacatcacgc ggcgcaaggt cggccagcgg
     3361 gtgcacaccg gtcatgaacg cgacgccgtc gtcgtcgcgg agcaccgcgc cttcgccgcg
     3421 tagagcttcg gacaggagcg gccgcggcat cgacgggtga tgcagcgcgg tcggatggaa
     3481 ctgcacgaac tccaagtctg cgcacgcgac gcccgccttg agcgcgagcg cgatgccgtc
     3541 gccggtcgag agcgtgggat tcgtcgtcac cgcgaagcac tgaccggcgc caccggtcgc
     3601 gagcacggtg tcggtagcgc ggacgaattc gacaccacca gtcgggttga gtgcgagcac
     3661 accctcgcag tggcccttct cgaccagcag ctcgatcgcg aaccagccct cacgcacttc
     3721 gatgtcgccg cgttcgacgg ccgcgacgag cgcacgctcg atctccgcgc ccgtcgcgtc
     3781 accacccgcg tgcacgaccc gcgccagcga gtgcccgcct tcgcgcgcga gcagcaactg
     3841 tggtccgtcg tcgtcgacct cggcgtcgaa ctgcgcgccc agcgccgcga gctcgcggac
     3901 gcggtcgggc ccttcggtca cgaggacgcg caccgcgtcg gcgtcgcaga ggccggcacc
     3961 ggcgacgagc gtgtcggcga gatgcagatc gggcgagtcc ggtggcgcca gcgcagccgc
     4021 gacgccgccc tgcgcgtagc gcgtcgccga gtgcgagagc tcgcccttgg tcaggacgag
     4081 gaccgacagc cctcgccgcg ccgcctgcag cgcggtcgtc aggccggcga ttccgctccc
     4141 gaggacgagc acatcgaaca cagcccgagc atacggggac aggccgtcag ctgttcgctc
     4201 caatccagac gacgttggaa ccggcgcgcg ccaccgcttc ggcctccggg atgggtcggt
     4261 tgcgctcgtc gacgtgcacg atctgcggct cgtagcgctc gagctcggcg gcatcgtagg
     4321 acgagtacgt gatgacgatg accttgtcgc cgggggcgac gagacgggcg gccgcgccgt
     4381 tgaggatgac gtcgcccggg ccaccggcca tcgcgtacgt ctcgaagcgc gcgccgttgt
     4441 cgatgtcgag cacgtgcacc tgttcgaact cgcggatgtc cgcgaggcgc atgagctcgg
     4501 ggtcgagcgt gatcgacccg acgtagtcga cacacgcgcc ggtcacagtg acgcggtgga
     4561 tcttcgactt catcatgaag cggttcattg cttgacctct ccgagatcgg cagcgacgtc
     4621 gtcgccgctg acagtgagtt gcacattgtc gatgagccgt gtgcggccca accgggccgc
     4681 aagcgcgacg agcacgtcgc cgtcgagcgt gtcgacgcgt gacaggtcat gcgcgtcgcg
     4741 cacttcgatg tactcgagcg cgaccagcgg ttcggtcgcg acagtcgact gcacgatcgc
     4801 gcgcagctgc tccggctcgc gctcgccggc ggccaccgcg tcggccgcgg cgcgcaacgc
     4861 gcggaacagc accggtgcgg cggcgtgctc gtccgccgtc aagtacgcgt tacggctcga
     4921 gagcgcgagg ccgtcggcct cgcgcaccag tgggcacccg tgcacggcgg ccgggaggtt
     4981 cagatcgagg gccatgcgcc gcacgaccgc gagctgctgg gcgtccttgc ggccgaaata
     5041 cgcgtcacac gggccgacga tcgagaacag cttcgtgacg acagtggtca cgccgtcgaa
     5101 gtgcgtcggg cgggacgcgc cgcacaggtc ggcggtcagg ccgtcgacgt gcacggtcgt
     5161 gactgcaccg cggggataca tctccgccac cgacggtgtg aacaccgcgt cggcgccctc
     5221 ggccgcgcac gcggcgagat cgcgctcgag gtcacgggga tacgcctcga ggtcttcggt
     5281 gggcccgaac tgcagcgggt tcacgaagat cgtcacgacg acgaagtcag tggcggcgcg
     5341 cgccgcgcgg atcaaggagc ggtggccctc gtgcaggaac cccatcgtcg gcaccaggcc
     5401 gacgcgttcg ccgcgagcgc gcgcgtcgtc gcacgcggcg cgcagatcgg caatggtcgc
     5461 aagctgtcgc acggatgcga acctcgtcgg ttccggccca actgggtccg gactggcggt
     5521 tcgggtcagt gcgaaatggt actacgcggg cttcagttcc cgtcggtggg cggctcgccg
     5581 gcgcggggcg cgaggacgtc gccaagcagc cggcccatcg cggtgtcgcg ccggcctgtg
     5641 aggcgcgcgg cctcgcgcgc gagcgagcgg tacgagtcgc gctccgcgga gtcgagtgcc
     5701 gcgaggtgtc cttcgacggt ggtgagatcg ccgcgttcga ccggacccgt gagcgcgttg
     5761 gcggggccga gacggaacgc gttgtcgacc gatgcctcgg ccagcggccc gaaggcagca
     5821 aacggcaccc ccgccgcggc ggcgagccgt tcgacctgac cgagcagcgc gacgagatgg
     5881 ttcgacgcga cgacggcagc cgcgtggtaa cgcacgcgat cggcgtccgc gaccgcgaac
     5941 gggcgcaacc ccaacgacat cgcgagctgc tcgacctcgg catcgcccgc gatcgcggcc
     6001 cacgagcccg caaggcgctc gacacccacg gatgtcgagg ggaacgactg caacgggtgc
     6061 agcgcgccga cccgcacgtc gggccgcttc tcccgcaacg cgtcgaagac atcgatgccc
     6121 ttcgaacccg ccaggtgcag cacgagcgcg tcgggctcga gggagtcggc aacggtgaga
     6181 gacgcgcctt cgatggcgcg gtcgggcgtg gcgatgatga cgagctgcgc gccctggccc
     6241 gctccggaga cgagcgtcgg ctgcgcggcg aggcacacgg ccgcggtggc ggtggacgcg
     6301 gcgtcggggg cccggcccgc aaccgcg
//
LOCUS       DASUGA010000292         3684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1274414, whole genome shotgun sequence.
ACCESSION   DASUGA010000292 DASUGA010000000
VERSION     DASUGA010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274414"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1303)
                     /gene="eno"
                     /locus_tag="VFX21_05790"
     CDS             complement(20..1303)
                     /gene="eno"
                     /locus_tag="VFX21_05790"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX5095503.1"
                     /translation="MSSIEDVSGREILDSRGNPTVEVEVELLSGARGRAAVPSGASTG
                     AHEAVELRDGEARFAGLGVLTAVDHVNTTLREELLGLEALDQRAIDAAMVAHDGTDNK
                     RRLGANAILGVSLAVAKAAADECGQSLFRYVGGANAHVLPVPMMNVLNGGAHADTNVD
                     IQEFMIAPVGAVSLSEAVRWCAETYHALKKLLHENGLSTAVGDEGGFAPNLPSNEEAL
                     KFLMRAIERAGYRPGDEIALALDAASTEFYAGGVYDLAGEGKKFSPSEFVDYLDALCD
                     RYPIVSLEDGMAEDDWDGWAALTARLGDRVQLVGDDLFVTNKERLQRGLDAGVANSIL
                     IKVNQIGTLTETLDTMALASRHAYTSMMSHRSGETEDVTIADLAVATNCGQIKTGAPA
                     RSDRVAKYNQLLRIEELLGSSATYAGASAFPRLSL"
     gene            complement(1431..2822)
                     /locus_tag="VFX21_05795"
     CDS             complement(1431..2822)
                     /locus_tag="VFX21_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HEX5095504.1"
                     /translation="MSTPRVVVAGLGPAGLDLLVPAARAAIDRIPVRFVRTAQHPAVD
                     ELVSAGVEFESLDRLYDAGDDLDTVYSAIASYVVDAALTHGEVLYAVPGSPSVAERTV
                     LLLRAATVEVEIIPGVSFADIAWARVGVDPLGGARVVDARAFAVEAAGGAGPMLIAQC
                     DSQFVLSDVKLALLEMLRPDHEVTVLQRLGLPDERVFTVALVDLDRDVAPDHLTSLFV
                     DSGELAVASELVRLVALAERLRGPGGCPWDAAQTHHSLRRHVIEEAYEVAEAIDVLPP
                     DAPGGDVDVRDYDALEDELGDLLFQVVIQSVLAAETGAFSIADVARRIHDKLIRRHPH
                     VFGDVRVGGADEVVTNWEQIKKSEKGDDSLVGGITASLPSLIAVQKLFRKADSIGLTP
                     RIAGDDVGAALAAIAQEARRNDVDADAELARWNTQFKARFQRMERAARDEGVDLANAD
                     PGTVDRLWAAARD"
     gene            complement(2819..>3684)
                     /locus_tag="VFX21_05800"
     CDS             complement(2819..>3684)
                     /locus_tag="VFX21_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5095505.1"
                     /translation="HISQGDFQDQLRDLGSHEVAVNYLSQGQGSHENSTDYSVEMSLA
                     TGWLDKLVTYQAAKIEAARLGVQVTDADLQAVREQVPGLEGKAGDILVEGEALSAAVA
                     GATPDEPSEQEVKAFYDEHEDALTECHGAVWVLAFATAGEAQQMVQQIEAMEGDVDEV
                     WQSANAQPNCLQYLPDELRREVEAAAADEVVGPLAFGDTYYVLGHAEDFAPTFEFWRD
                     QLASALSNPDAAISLRKFLMTVHIAPQYGSWEGALLQVQAPPVPEPNTSREGGTGTTG
                     DVLVAGDGTTG"
BASE COUNT          539 a         1295 c         1256 g          594 t
ORIGIN      
        1 ctcacccgcc catggtccct cagagggaca agcgcgggaa cgccgacgca ccggcgtacg
       61 tggccgaact gcccagcagt tcctcgatcc ggagcagctg gttgtacttg gcaacccggt
      121 cggagcgagc cggcgcaccg gtcttgatct ggccgcagtt ggtcgcgacc gcgaggtcgg
      181 cgatcgtgac gtcctccgtc tcgccgctgc ggtgcgacat catcgacgtg tacgcgtgcc
      241 ggctcgcgag cgccatcgtg tcgagcgtct cggtgagcgt cccgatctgg ttcaccttga
      301 tcaggatgga gttggcgacg ccggcgtcga gcccgcgctg caggcgttcc ttgttcgtca
      361 cgaacaggtc gtcgccgacg agctgcaccc ggtcgccgag gcgcgcggtg agcgccgccc
      421 agccgtccca gtcgtcctcg gccatgccgt cttcgagcga gacgatcggg tagcgatcgc
      481 acagggcgtc gagatagtcg acgaactccg acgggctgaa cttcttgcct tcgccggcga
      541 ggtcgtacac gccgccggcg tagaactcgg tcgacgcggc gtcgagcgcg agcgcgatct
      601 cgtcgccggg gcggtacccc gcgcgctcga tggcgcgcat caagaacttg agcgcttctt
      661 cgttcgacgg caagttcggc gcgaacccac cttcgtcgcc gactgcggtc gacagaccgt
      721 tctcgtgcaa cagcttcttc agcgcgtggt atgtctccgc gcaccagcgc accgcctccg
      781 acaagctcac cgcgcccacc ggggcgatca tgaactcctg gatgtcgacg ttcgtgtccg
      841 cgtgcgcgcc accgttcagc acgttcatca tcggcacggg gagcacgtgc gcgttcgcgc
      901 cgcccacgta gcggaacagc gactggccgc attcgtcggc cgcggccttg gcgaccgcga
      961 gcgacacgcc gaggatcgcg ttcgcgccga gccgccgctt gttgtcggtg ccgtcgtggg
     1021 cgaccatcgc ggcgtcgatc gcgcgctggt cgagcgcctc gagaccgagc agctcctcgc
     1081 gcagcgtggt gttgacgtgg tccacggccg tgagcacgcc caagccggcg aagcgggcct
     1141 cgccgtcgcg gagctcgacc gcctcgtggg cgccggtgct ggcgcccgac gggaccgcgg
     1201 cccggccccg ggccccggag aggagctcca cctccacctc gacggtgggg ttgccgcggg
     1261 agtcgaggat ctcccggccg ctgacgtcct cgatgctgct cacgtgaact cctgtgaaag
     1321 gtgtggcgaa tgtaatcggt tgagggccgc gatcggtgtc acattccgtg gaatgaagtt
     1381 caccgaacgt gcgttcggcg ggcgagcctc ggtttccggt cccgccattc ctagtcgcgg
     1441 gcggcggccc agagccggtc gacggtgccg ggatcggcgt tcgcgaggtc gacgccctcg
     1501 tcgcgtgcgg cgcgctccat gcgttggaag cgcgctttga actgcgtgtt ccaccgcgcg
     1561 agctcggcat cggcgtcgac gtcgttgcgc cgtgcctcct gcgcgattgc ggccagcgcc
     1621 gcgccgacgt cgtcacctgc gatacgaggc gtcagaccga tcgagtcggc cttgcggaac
     1681 agcttctgca ccgcgatcaa cgacggcagg ctcgcggtga tcccgccgac cagtgagtcg
     1741 tcgcccttct ccgacttctt gatctgctcc cagttcgtca cgacctcgtc ggcgccgccg
     1801 acccgtacgt cgccgaacac atgtgggtgg cggcggatga gcttgtcgtg gatccggcgc
     1861 gccacgtcgg cgatgctgaa cgcgccggtc tcggccgcga gcaccgactg gatgacgacc
     1921 tggaacagca agtcgccgag ctcgtcttcg agcgcgtcgt agtcgcggac gtcgacgtca
     1981 ccgccgggcg cgtcgggcgg cagcacgtcg atggcttccg ccacctcgta ggcctcctcg
     2041 atcacgtggc ggcgcaacga gtggtgggtc tgcgcggcgt cccacgggca cccgccgggg
     2101 ccgcgcaacc gctcggccaa cgcgaccagg cgcaccagct ccgacgccac ggccagctcg
     2161 cccgaatcga cgaacagcga cgtcaagtga tcgggcgcga cgtcgcggtc gaggtcgacg
     2221 agtgcaaccg tgaacacgcg ctcgtccggg agaccgaggc gctggagcac ggtcacttcg
     2281 tgatccggtc gcagcatctc gagcagtgcg agcttgacgt cggagaggac gaactgtgag
     2341 tcgcactgcg cgatcagcat cgggcccgcg ccaccggcgg cttcgaccgc gaacgcacgc
     2401 gcgtcgacga cgcgcgcgcc tccgaggggg tcgacaccga cgcgcgccca cgcaatgtcg
     2461 gcgaacgaaa caccggggat gatctcgacc tcgaccgtcg ccgcccgcag caagagcacg
     2521 gtgcgctccg cgacgctcgg actgcccggc accgcgtaca gcacctcacc gtgcgtcagc
     2581 gccgcgtcga ccacatacga cgcgatcgcg ctgtagaccg tgtcgaggtc gtcacccgcg
     2641 tcgtagaggc gatcgagcga ctcgaactcg acgcccgcgc tcacgagctc gtcgacggcg
     2701 ggatgttggg cagtacggac gaaccggacg gggatgcgat cgatcgcggc gcgcgccgcg
     2761 ggcacgagca ggtcgaggcc ggcagggccg agcccggcaa cgacgactcg cggcgtgctc
     2821 acccggtcgt gccgtcgccg gcgacgagca cgtcgcctgt cgtccccgtc ccgccctcac
     2881 ggctcgtgtt cggctcgggc acgggtggtg cctgcacctg gagcagcgcg ccctcccacg
     2941 agccgtactg cggagcgatg tggacggtca tgaggaactt gcggaggctg atcgccgcgt
     3001 ccgggttcga gagcgcgctc gcgagctggt cgcgccagaa ctcgaacgtc ggcgcgaagt
     3061 cctcggcgtg cccgagcacg tagtacgtgt caccgaaggc caggggaccg acgacttcgt
     3121 cggccgcggc cgcttcgact tcgcgtcgga gctcgtcggg caggtactgc agacagttcg
     3181 gctgcgcgtt ggccgactgc cagacctcgt cgacgtcgcc ttccatcgct tcgatctgct
     3241 gcaccatctg ctgcgcttcg ccggcggtcg cgaacgcgag cacccacacc gcgccgtgac
     3301 actcggtcag tgcatcttcg tgctcgtcgt agaacgcctt gacctcttgc tccgatggct
     3361 cgtcgggggt cgcgccggca accgcggccg acagcgcttc gccctccacc aagatgtcgc
     3421 ccgccttgcc ctcgagcccg ggaacttgtt ctcgtacggc ctgcaggtcg gcgtcggtca
     3481 cctgcacacc gaggcgcgcc gcttcgatct tcgcggcttg ataggtgacg agcttgtcga
     3541 gccacccggt cgcgagcgac atctcgaccg agtagtccgt cgagttctcg tggctgccct
     3601 gaccctgcga cagatagttg acggctacct cgtgactccc gaggtcgcgg agctgatcct
     3661 ggaagtcgcc ctgcgagatg tgga
//
LOCUS       DASUGA010000293         3480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_682158, whole genome shotgun sequence.
ACCESSION   DASUGA010000293 DASUGA010000000
VERSION     DASUGA010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682158"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            657..2444
                     /locus_tag="VFX21_05805"
     CDS             657..2444
                     /locus_tag="VFX21_05805"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HEX5095506.1"
                     /translation="MITKPLWMACVGAAIAGGVSAQQMFPLPATTTDTTSTESTAPFL
                     APMGVKQTLITDRKTLSVRGLPSSFSNWDMVAFDPTARYVFVPAEVGSGAGVFRYDTQ
                     SGAIATLMRGNESGTRTSDPKIWSATNDDFARIDPASWSPWGSIITGEETTGGRLFEI
                     TNPMASGNFSVKWRDNIPAVSHEGLRFDKDGALYFVDEDNSGSIYKFVPKNKGDLSVG
                     QSFVLSVDAFANNKSAAPNESWSSTANRATTRTGFATWVPMTDADGKALTTANPFAFV
                     TTTGGRTAADELRGTPYGRPEDVDFNYLASGNQALYFTATSENRVYSVELHDATRAIV
                     RVFCDFDTINLATGKDVNPLQNDPYTSPGPDSSTIFNNPDNLAVDAYGNVYVIEDANP
                     GDIWKCYDANKDGVAESVGLFVSLGVAGCEPTGLIQDPTNRGRMICCIQHPSSGNDAL
                     WAFDVCPFPGSGADLHLGIGINVPANGGAGEFVRGVETGDWVTFHVSSRSAYVGAPYA
                     LLLQFPSNTPRQFLPGIWMDLGQPMIVLAGGSQLNFPTVLSPFGASLTVQVPPGLTGL
                     TVMSQAVSSARNVNGGLVSFSDAFETCFK"
     gene            2715..>3480
                     /locus_tag="VFX21_05810"
     CDS             2715..>3480
                     /locus_tag="VFX21_05810"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX5095507.1"
                     /translation="MSMRPNFVTATSLAFSLLLVTALQAQDRLLADGIRAPDFTSKDV
                     NGRDVRTSDYTGKVVVLDFWATWCTPCLQSLPHVQEIAKSHRDAGVVVLAVCTSDSRA
                     RFDAWVETNAARYPDVVFTCDPHDRGSDSFDERASKKLYHVSGLPTKFVIGRDGKVAM
                     GIVGSEKDDVRLEAGLARAGIAIDAAVARAGEAMAAKITKEEAARAAAARMAPSFFPS
                     AGKMKSGDEVPDLTLQRADGTEFALSSLRGKPIVLAV"
BASE COUNT          589 a         1246 c         1126 g          519 t
ORIGIN      
        1 gagccccgag tgcagcatct gcagcggccg gagattgctc gcccccatcg tcgccccgta
       61 gaggaaaccg gcgacgcaga gcagcacggc cagctcgtgg ccgcggtacc tggagaccaa
      121 ctcgtccggc cgcggcgcgc gtagcaggtc gtcgacggca gcgagcttca cgacaggagc
      181 tggcggagcg ccgcacacgg tgcgcatgcg gttcacgcgc gcgcagccgc gtctcgcgaa
      241 gaaggtcggt gagctccggc gcggattgct cgcggacctg ctcggcgaag aggtggcgga
      301 agcggacgag gcctgatcgg cgacgacccg ttcgacgggc acggacgatc gacgccgcgc
      361 tgcatcgcga tgcgtgccgc acctgactgc atcgagtgat gcgcgtcgcg aacgttcgtc
      421 tcgcacgcgt gatcaccgca cgtctcgtct cgcgagtgcg cgatgaagaa cgagcgtgtg
      481 cacgatgtgg atcgtgtgag ccgatgtgca cgcgagtcgt tcgcatcgcg tgcacgcgct
      541 cgtcgattga cggtggactt cacgagggat tggcatgcgg tcaacgaact ccgtctagcg
      601 tctcgagcct tccggggaaa accccacagc acctcagaca accaaacgca caacacatga
      661 tcaccaagcc cctttggatg gcctgcgtcg gcgcagcgat cgccggcggc gtgtccgcac
      721 agcagatgtt cccgcttccc gcgacgacca cggacacgac gtcgacggag agcaccgcgc
      781 cgttcctggc accgatgggc gtcaagcaga cgctcatcac cgaccgcaag acgctctccg
      841 tgcgcggtct gccttcgagc ttcagcaact gggacatggt cgcgttcgat ccgaccgctc
      901 gctacgtctt cgttcccgca gaggtcggca gcggcgccgg cgtgttccgc tacgacacgc
      961 agagcggggc catcgcgacc ctgatgcggg gcaacgagag cggcacgcgt acgagcgacc
     1021 ccaagatctg gagcgcgacg aacgacgact tcgcccgcat cgatccggcg tcgtggagcc
     1081 cctggggcag catcatcacg ggcgaggaga cgaccggtgg ccggctcttc gagatcacca
     1141 acccgatggc gtcgggcaac ttctcggtga agtggcgcga caacatcccc gcggtctccc
     1201 acgagggact gcgcttcgac aaggacggcg cgctctactt cgtcgacgag gacaacagcg
     1261 gctcgatcta caagttcgtc ccgaagaaca agggcgacct gagcgtcggc cagtccttcg
     1321 tcctcagcgt cgacgcgttc gccaacaaca agagcgccgc cccgaacgag agctggagct
     1381 ccaccgcaaa ccgcgcgacg acccgcaccg ggttcgcgac ctgggtgccg atgaccgacg
     1441 cggacggcaa ggcgctcacc actgcgaatc ccttcgcgtt cgtgacgacg accggaggcc
     1501 gcacggcggc ggacgagctc cggggcacgc catacggccg tcccgaggac gtcgacttca
     1561 actacctcgc gagcggcaat caggcgctct acttcaccgc gacgagcgag aaccgcgtct
     1621 actccgtcga gctccatgac gcgacgcgcg cgatcgtccg cgtgttctgc gacttcgaca
     1681 cgatcaacct cgccaccggc aaggacgtca acccgctgca gaacgatccc tacacgagcc
     1741 ccggccccga cagcagcacg atcttcaaca acccggacaa cctcgccgtc gatgcctacg
     1801 gcaacgtcta cgtcatcgag gacgcgaacc cgggcgacat ctggaagtgc tacgacgcca
     1861 acaaggacgg tgtggcggag tcggtggggc tgttcgtgag cctgggtgtt gctggttgcg
     1921 agccgacggg tctcatccag gatccgacca accgcggccg catgatctgc tgcatccagc
     1981 acccgtcgag cggcaacgac gcgctgtggg cgttcgacgt ctgcccgttc ccgggcagcg
     2041 gcgcggacct gcatctcggc atcggcatca acgtgccggc gaacggtggg gcgggcgagt
     2101 tcgtccgcgg cgtggagacc ggtgactggg tcacattcca cgtgtcctcg cgcagcgcct
     2161 acgtcggcgc gccctacgcg ctgctgttgc agttcccgag taacacgccg cgccagttcc
     2221 tgccgggcat ctggatggac ctcgggcagc cgatgatcgt cctcgcgggc ggctcgcagc
     2281 tgaacttccc cacggtgctc tcgccgttcg gcgcttcgct caccgtgcag gttccgcccg
     2341 ggctcacggg cctgacggtc atgtcgcagg ccgtgtcttc cgcgcggaac gtcaacggcg
     2401 gcctcgtctc gttctccgac gcgttcgaga cctgcttcaa gtaggcctca tgcagtcggt
     2461 cggcgcggtg gaagaccacc gcgccgcggc ttggagccgt cgcgctactt cggcggcggc
     2521 ggcttcttcg gcccggcggc cgcaagcagc gactggcaca gctcgagggc ccgcgcgctg
     2581 ccgggagcca ccgttccgcc cctgagtccc cccgcccccg cgtgccgagg atccgtgctg
     2641 gtccttggca tccgcacatc ggcgcgacag actcggtcac acacggcgag agcacgactc
     2701 cgcacccccc aaccatgtcc atgcgcccca acttcgtcac ggcgacgtcg ctcgccttct
     2761 ccctgctgct cgtgaccgca ctgcaggccc aggaccgcct gctcgcggac ggcattcgcg
     2821 ccccggactt caccagcaag gacgtcaacg gccgcgacgt gcgcacgagc gactacaccg
     2881 gcaaggtcgt cgtcctcgac ttctgggcga cgtggtgcac gccgtgcctg cagtcgctcc
     2941 cacacgtcca ggagatcgcc aagtcgcacc gagatgccgg cgtcgtcgtc ctcgccgtct
     3001 gcacgagcga ttcgcgcgcc cgcttcgacg cctgggtcga gacgaacgcc gcgcgctatc
     3061 ccgacgtcgt gttcacgtgc gatccgcacg atcgcggcag cgactcgttc gacgaacgcg
     3121 cctccaagaa gctctaccac gtgtccggcc tgccgacgaa gttcgtcatc ggacgcgacg
     3181 gcaaggtggc catgggcatc gtcggctcgg agaaggacga cgtccggctc gaggcgggcc
     3241 tcgcgcgcgc cggcatcgcg atcgacgccg ccgtcgcccg cgccggcgag gcgatggccg
     3301 cgaagatcac caaggaagaa gccgcccgtg cggcagccgc gcgcatggct ccgtcgttct
     3361 tccccagcgc cgggaagatg aagagcggcg acgaagtccc cgacctcacc ctgcagcgcg
     3421 cagacggcac cgagttcgcc ctgtcgtccc tgcgcggcaa gcccatcgtg ctcgcggtcg
//
LOCUS       DASUGA010000294         4369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1895194, whole genome shotgun sequence.
ACCESSION   DASUGA010000294 DASUGA010000000
VERSION     DASUGA010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1895194"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VFX21_05815"
     CDS             complement(<1..891)
                     /locus_tag="VFX21_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX5095508.1"
                     /translation="MDDAGLAGKGEPLEHSYISGGSQNEIYELRRGNLHCAMRIPPST
                     APESRDDGIMREWRIIEALNGTDVPHTEAVAVCTDKSVLGRTFYLMGFVDGWSPMGLE
                     GGRWPAPFDTDLDARRGLSFQLVEGIALLSKVDWQAKGLQDLGRPDGFHERQVDRWTS
                     FLDRIKGRELPGFDEAAAWLRAHRPIDFVPGLMHGDYQFANVMFCHGTPARLAAIVDW
                     EMGTVGDPKLDLGWTVQGWPADTTSSDAGSLGYVDYRGMPSRDEVVAHYAKVSGRQVD
                     DLDYYLVLAKWKLAVVLEQGY"
     gene            complement(933..2234)
                     /locus_tag="VFX21_05820"
     CDS             complement(933..2234)
                     /locus_tag="VFX21_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5095509.1"
                     /translation="MAWDFETEPEFQAKLDWVDAFVREEVEPLDLAFPGLAFTPLDDN
                     RRKVIDPLKEEVRRQGLWATHLGPELGGQGFGQLKLALLNEILGRSSWAPIVFGCQAP
                     DTGNAEIIAHYGTPEQKEKYLRPLLEGEMFSCYSMTEPHAGADPTMFTTRAEKDGDEW
                     VINGWKFFSSNAKTASFLIVMAVSNPEISAYQGMSMFLVPTDTPGINIVRNVGLGGES
                     RDEGSHALIHYDNVRVPAEGLLGGEGQAFAIAQTRLGGGRIHHAMRTIGMAQRALDMM
                     CERALSRETQGSLLADKQFVQGYIADSYAQLIQFRLFVLYTAWEIDKYNDYKRVRKDI
                     ATAKVVMPTVLHDIAWRAMQVHGALGVSNEMPFSNMVLAAAVMGLADGPTEVHKVTVA
                     RQVLRGYKPSDDLWPTQHIPKLRDAARAKFAEYMEHEVGNL"
     gene            complement(2296..3948)
                     /locus_tag="VFX21_05825"
     CDS             complement(2296..3948)
                     /locus_tag="VFX21_05825"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5095510.1"
                     /translation="MTADIVIRGGTVYDGTGAPGRAADVAIADGVVTEIGANLHGERE
                     LDASGCAVTPGFIDIHTHYDAQVFWDPCLRPTSFHGVTTVVAGNCGFAIAPTRPEFRD
                     TIVRTLENVEDMDPATLTAGVDWDFETFPEYKASIQRRGTLLNFTAYCGHSALRLYVM
                     GDAAYERAATPDEVAQMCALVREAIELGAAGFSTSFSYAHRGVDGKPVPSRYSDKAEV
                     DALFRAAGEAGKGVILITPGEQCTYTDVYEWQPQVGRPFTYPLFASPGGKHLEPVRLH
                     EEGLARGAQVWPQVTPRPLTMQFTMEDAYNLNTGEVFGELMKTSRRVRLAAYRDPAWR
                     AEAAADLEHSKMKPRWETFEVSESSKFPELEGRRVTELAAERGMSPFDVLCEIAVADD
                     LKTRFRTYIANDDVQAVSHLLTHESVALGLSDAGAHVDQLCDAPLPTDLLGNWVRERE
                     VMALERAVHKLTGEPASVFGFVRRGELRAGSWADVCVFDPATIGPGPTRRVRDFPADA
                     ERLTAEEPSGLRHVLVNGTPIRVDEKQLDAPDLRPGVWPENA"
     gene            complement(3938..>4369)
                     /locus_tag="VFX21_05830"
     CDS             complement(3938..>4369)
                     /locus_tag="VFX21_05830"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5095511.1"
                     /translation="LDEPLAIMIFSGALERHPGFTLVLAESGIGWLPYFLTRMDLEWE
                     SLREKLDYAPSVPPSELFRRQVIATFEEEPSAVQFIPMLGAESCMWASDYPHTDSTFP
                     NSRATIEETLGALPADDIRKVTGANCAELYGFDAKGLDDDS"
BASE COUNT          689 a         1493 c         1444 g          743 t
ORIGIN      
        1 gtaaccctgc tccaacacga ccgcgagctt ccacttcgcg agcacgaggt agtagtcgag
       61 gtcgtcgacc tgccggccgg acaccttggc gtagtgcgcg acgacttcgt cacgcgacgg
      121 catgccgcga tagtcgacgt agccgaggct cccggcgtcg gacgaagtcg tgtcggcggg
      181 ccaaccttgc acggtccacc cgagatcgag cttcgggtcg ccgaccgtgc ccatctccca
      241 gtcgacgatc gccgcgagcc gcgccggcgt gccgtggcag aacatgacgt tcgcgaactg
      301 gtagtcgccg tgcatgaggc cgggaacgaa gtcgatgggg cggtgcgcgc gcagccacgc
      361 ggcggcttcg tcgaagccgg gcaattcgcg gcccttgatg cggtccaaga acgatgtcca
      421 gcggtcgacc tgccgctcgt ggaagccgtc aggtcgtccg agatcctgca agcccttcgc
      481 ctgccagtcg accttcgaca acaacgcgat gccctcgacg agttggaacg aaagaccgcg
      541 gcgggcgtcg aggtcggtgt cgaacggcgc cggccaccgg ccgccctcca gccccatcgg
      601 cgaccagccg tcgacgaaac ccatcaaata gaacgtgcgt ccgagcaccg acttgtcggt
      661 acagacggcg acggcttcgg tgtgcggcac gtcggtgccg ttcagcgcct cgatgatgcg
      721 ccactcgcgc atgatgccgt cgtcgcgtga ttcgggtgcg gtcgaaggcg ggatgcgcat
      781 cgcgcagtgc aggttgccgc gccgcagctc gtagatctcg ttctgcgatc cgccggagat
      841 gtagctgtgt tcgagcggct cgcccttgcc ggccaggccg gcatcgtcca tccacgaacc
      901 gaggcggtcg gtgtcgatcg tcggtgtggt ggtcacagat tcccgacctc gtgctccatg
      961 tactcggcga acttcgcgcg cgcggcgtcg cgcagcttcg gaatgtgttg cgtcggccag
     1021 aggtcgtcgc tcggcttgta accgcgcaat acctgccgcg ccaccgtcac cttgtgcacc
     1081 tcggtgggac cgtcggccag ccccatgacc gcggccgcga gcaccatgtt cgagaacggc
     1141 atctcgttgc tcacaccgag tgcgccgtgt acctgcatcg cgcgccacgc gatgtcgtgc
     1201 aggaccgtcg gcatgacgac cttggcggtg gcgatgtcct tgcggaccct cttgtagtcg
     1261 ttgtacttgt cgatctccca cgcggtgtac agcacgaaca gccggaactg gatcagctgc
     1321 gcgtaggagt cggcgatgta gccctgcacg aactgcttgt cggccagcag cgagccctgc
     1381 gtctcgcggc tcagtgcgcg ctcgcacatc atgtcgagcg cccgctgcgc cataccgatc
     1441 gtgcgcatcg cgtggtggat acgtccgccg ccgagtcgag tctgcgcgat cgcgaacgcc
     1501 tgaccctcac cgccgagcag gccctccgcg ggtacgcgca cgttgtcgta gtgaatcagt
     1561 gcgtgcgaac cctcgtcgcg cgactcacca ccgaggccga cgttgcgcac gatgttgatg
     1621 ccgggcgtgt cggtcggcac gaggaacatc gacatgcctt ggtacgcgct gatctcgggg
     1681 ttgctcaccg ccatgacgat gaggaacgac gcggtcttcg cgttcgacga gaagaacttc
     1741 cagccgttga tgacccactc gtcgccgtcc ttctcggcgc gcgtcgtgaa catcgtcggg
     1801 tccgcgcccg cgtggggctc ggtcatcgag tagcacgaga acatctcgcc ttcgagcagc
     1861 gggcggaggt acttctcctt ctgctcgggc gtgccgtagt gggcaatgat ctccgcgttg
     1921 cccgtgtccg gcgcttggca accgaacacg atcggcgccc acgacgagcg accgaggatc
     1981 tcgttcaaca acgcgagctt gagttggcca aagccctggc cgcccagctc gggacccaag
     2041 tgtgtcgccc agagaccttg gcgccgcacc tcttctttca gcgggtcgat gaccttgcga
     2101 cggttgtcgt cgagcggtgt gaacgcgaga cccgggaacg cgaggtcgag tggttcgact
     2161 tcttctctta cgaacgcgtc gacccagtcg agcttcgctt ggaactcggg ctcggtctcg
     2221 aaatcccacg ccatgaacgt ctccctcgtc gacgccgcaa gcgtacgcga actcctgaca
     2281 cgcgtgtcag gtcgatcagg cgttctcggg ccacacgccg ggccggagat cgggggcgtc
     2341 cagctgcttc tcgtcgacgc gaatcggtgt gccgttcacg aggacgtgac gcaaaccgga
     2401 cggctcctcc gcggtcagcc gctcggcgtc ggccgggaaa tcacgcaccc gccgcgtcgg
     2461 ccccggaccg atcgtcgccg gatcgaacac gcacacatcc gcccacgatc ccgcgcgcag
     2521 ctcgccccgg cgcacgaacc cgaacacaga tgcgggttcg ccggtcagct tgtgcaccgc
     2581 ccgctccagc gccatgacct cgcgctcgcg cacccagttg ccgagcaggt cggtggggag
     2641 cggcgcgtcg cacaactggt cgacgtgcgc acccgcgtcg gacagcccga gcgcgaccga
     2701 ctcgtgcgtg agcagatgac tcaccgcctg gacgtcgtcg ttggcgatgt aggtgcgaaa
     2761 gcgcgttttc aaatcgtcgg caaccgcgat ctcgcacagc acgtcgaacg ggctcatgcc
     2821 gcgctccgcc gcgagctcgg tgacccgacg cccttcgagc tcgggaaact tcgatgactc
     2881 ggacacttcg aacgtctccc agcggggctt catcttcgag tgctcgaggt cggcggccgc
     2941 ttccgcgcgc cacgcggggt cgcggtacgc ggcgagccgc acgcggcggc tggtcttcat
     3001 caactcgccg aacacctcgc cggtgttgag gttgtacgcg tcctccatcg tgaactgcat
     3061 ggtgagcggg cgcggcgtga cctgcggcca gacctgcgcg ccgcgggcga ggccttcctc
     3121 gtggagccgc accggctcca ggtgcttgcc cccggggctc gcgaacagcg ggtacgtgaa
     3181 cggccgcccg acctgcggct gccactcgta cacatctgta taggtgcact gctcgccggg
     3241 cgtgatgagg atgacgccct tgccggcctc ccctgcggcg cggaacagcg cgtcgacctc
     3301 ggccttgtcg gagtaccggc tcgggacggg cttgccgtcg acgccgcggt gcgcgtacga
     3361 gaagctcgtc gagaacccgg cggcgccgag ttcgatcgct tcgcgtacga gcgcgcacat
     3421 ctgcgccacc tcgtcgggcg tcgccgcgcg ctcgtacgcg gcgtcgccca tgacgtacaa
     3481 ccgcagcgcg ctgtgcccgc agtaggcggt gaagttgagc agcgtcccgc gtcgctgaat
     3541 cgacgccttg tactccggaa acgtctcgaa gtcccagtcg acgcccgcgg tgagcgtcgc
     3601 ggggtccatg tcttccacgt tctcgagcgt gcgcacgatc gtgtcgcgga actcggggcg
     3661 ggtcggtgcg atcgcaaatc cgcagttgcc ggccacgacc gtggtgacgc cgtggaacga
     3721 cgtcggccgc aggcaggggt cccagaacac ctgcgcgtcg tagtgcgtgt ggatgtcgat
     3781 gaaacccggc gtgaccgcgc aacccgaggc gtcgagctct cgttcgccgt ggaggttcgc
     3841 accgatctcg gtgaccacac cgtccgcgat cgcgacgtcg gcggcgcggc ccggggcacc
     3901 ggtgccgtcg tagacggtgc caccgcggat cacgatgtca gctgtcatcg tcgaggccct
     3961 tcgcatcgaa gccgtacagc tcggcgcaat tcgcgcccgt gaccttgcgg atgtcgtcgg
     4021 ccggcaacgc gccgagcgtc tcctcaatcg tggcgcgcga gttcgggaac gtgctgtcgg
     4081 tgtgcgggta gtcggaggcc cacatgcacg actcggcgcc gagcatcggg atgaactgca
     4141 ccgcggacgg ttcctcctcg aacgtcgcga tcacctggcg ccggaacaac tcactgggcg
     4201 gcacgctcgg cgcatagtcg agcttctcgc gcaggctctc ccactcgagg tccatacgag
     4261 tgaggaagta cggcagccaa ccgatccccg actccgcgag cacgagtgtg aatcccgggt
     4321 gccgttcgag cgcgcccgag aagatcatga tcgccagcgg ttcgtcgag
//
LOCUS       DASUGA010000295         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1392673, whole genome shotgun sequence.
ACCESSION   DASUGA010000295 DASUGA010000000
VERSION     DASUGA010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392673"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="VFX21_05835"
     CDS             <1..1275
                     /locus_tag="VFX21_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095512.1"
                     /translation="FCMQTHIPYLGLVGGIVLGATVWVVVRAVRQRDNQKLRRDLVRY
                     GIVAVVLGLVLWTPPVLDEIVHEPGNITTIREHFSDPPETPVGLKDGVYVLGTQLNPW
                     RFVSEVLVRDGKPRPVDGSMAPGLLLVAAWAAVAVLSAWKLRVRALVALHVVIGIALV
                     LGVISAARIFGTVWYYLLLWAWGLVALMLLVIGWTVVELVRPRLDDGARRTAARWGAG
                     ALGAIVLVTSVVFAVDAADVDVQSPRLNESLGRVVGPTADALAAREAAGARGPYLVSW
                     LPDPLAIGSQGFGLLNELVRRGYDVNAATVFRPGATRYHVMDPATATLEVHLATGPAI
                     DAFRAEHPDFEEVASYDPRSSEEQAEAARLRDEVIDELEAAGLDASAKDVDLNLFMVG
                     VDQQVPAATRDKIARIVELGMPVAVFVGSPVE"
     gene            1272..>2351
                     /locus_tag="VFX21_05840"
     CDS             1272..>2351
                     /locus_tag="VFX21_05840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5095513.1"
                     /translation="MSGRRFAWTLGAIAGIALIVRVAYVLIERRDIVLAGDQYFYHRG
                     ANLLVDGKGFISPFFYDLGQRVQAAEHPPLYLLFLSIPSALGMKSVLVHLLWSCLPST
                     ATVVLVGYLGRAVWSARAGLVAAGIFAVYPNAWAPAGLLQAEVLSMFFVALSLVLGCR
                     YWRAPSAGRLALVGVAIGLGALARSELILLVPLLVAPLAWKTAARSARDRLVWFGAAT
                     AAVIALLMPWSIYNATRFEHPVLLSTQFDALLAATNCDSVYYGPFAGYYDNNCTKDAY
                     ARAGLGSDDDQSEQSIVFRRAAVEYATEHAGRWPYVASTRLARSVGLYKADLSTRLDI
                     FLETRESWVAWSALYSFYALALLAIA"
BASE COUNT          294 a          781 c          845 g          431 t
ORIGIN      
        1 ttctgcatgc agacgcacat tccgtatctc gggttggtcg gcggcatcgt gctcggcgcg
       61 acggtgtggg tcgtcgtgcg cgccgtacgt cagcgcgaca accagaagct gcgccgtgat
      121 ctcgtccggt acggcatcgt cgcggtggtc ctcggcctcg tgctgtggac gccgccggtg
      181 ctcgacgaga tcgtgcacga gcccggcaac atcacgacga tccgcgagca cttcagcgat
      241 ccgccggaga cgccggtcgg gctgaaggac ggcgtctacg tcctcggcac gcagctgaac
      301 ccctggcgct tcgtcagcga agtgctcgtg cgcgacggca agccgcgccc cgtcgacggt
      361 tcgatggcgc cgggactgtt gctcgtcgcc gcatgggccg cggtcgcggt gctcagcgcg
      421 tggaaactgc gggtgcgcgc actcgtcgcg ctccacgtcg tgatcgggat cgcgctcgtg
      481 ctcggtgtga tctccgcggc gcgcatcttc ggcaccgtct ggtactacct gttgctctgg
      541 gcgtggggcc tcgtcgcgct catgctgctc gtgatcgggt ggacggtcgt ggagctcgtc
      601 cggccccggc tcgacgacgg cgcgcgccgc accgcggcgc gctggggcgc gggcgcgttg
      661 ggtgcgatcg tcctggtgac aagcgtcgtg ttcgcggttg atgccgccga cgtcgatgtg
      721 cagtcgccgc ggctcaacga gtcgctgggc cgggtcgtcg gcccgaccgc cgatgcgctc
      781 gccgcgcgcg aggccgcggg cgcacgcggt ccgtacctgg tgtcgtggct cccggatccg
      841 ctcgcgatcg gttcgcaggg cttcggtctg ctgaacgagc tcgtccggcg tggctacgac
      901 gtgaacgcgg cgaccgtctt ccgcccgggc gcgacgcgct accacgtgat ggatccggcc
      961 accgcaacgc tcgaggtgca cctcgcgacc ggcccggcga tcgacgcgtt ccgtgccgaa
     1021 caccccgact tcgaagaagt cgcgtcgtac gacccacgct cgtccgagga gcaggccgaa
     1081 gcggcccggc tacgcgacga ggtgatcgac gagctggaag ccgcgggact ggacgcgtcg
     1141 gcgaaggacg tcgacctcaa cctgttcatg gtcggtgtcg accagcaggt cccggcggcg
     1201 acacgcgaca agatcgcccg catcgtggag ctggggatgc ccgtcgccgt cttcgtcggg
     1261 tcgccggtcg agtgagcggc cggcgcttcg cgtggacgct cggcgcgatc gcgggcatcg
     1321 cgttgatcgt gcgcgtcgcg tacgtgctga tcgagcggcg cgacatcgtt ctcgcgggcg
     1381 accagtactt ctatcaccgg ggcgcgaacc tgctcgtcga cggcaagggc ttcatctcgc
     1441 cgttcttcta cgacctcggg cagcgcgtgc aagcggccga acatccgccg ctgtatttgc
     1501 tgttcctgtc gatcccgtcg gcgctcggga tgaagagcgt gctggtgcac ttgttgtggt
     1561 cgtgcctgcc gagcacggcg acggtggtac tggtcggcta cctcgggcgc gccgtgtggt
     1621 cggcgcgcgc cggattggtc gcggccggca tcttcgcggt gtacccgaac gcatgggcgc
     1681 cggccggtct gttgcaggcc gaagtgctgt cgatgttctt cgttgcgctg agcctcgtcc
     1741 tcgggtgccg gtactggcgc gcgccgagtg ccggtcgcct cgcgctggtc ggtgtcgcga
     1801 tcgggctggg cgcgctcgcc cggtcggagc tcatcctcct cgtgccgttg ctcgtcgcgc
     1861 cgttggcgtg gaagacggcg gcgcgatcgg cacgagaccg gctcgtgtgg ttcggcgccg
     1921 cgaccgcagc agtgatcgcg ctgctcatgc cgtggtcgat ctacaacgca acgcgcttcg
     1981 agcacccggt gttgttgagc acgcaattcg atgcgttgct cgcggccacg aattgcgatt
     2041 ccgtgtacta cggcccgttc gcgggctact acgacaacaa ctgtacgaag gacgcgtacg
     2101 cgcgtgccgg gctcggttcg gatgacgacc agtcggagca gtcgatcgtc ttccggcggg
     2161 cggcggtcga gtatgcaacg gagcacgcgg gtcgctggcc gtacgtcgcg agcactcgtc
     2221 tcgcgcgcag cgtcggtctc tacaaggccg acctgtcgac acgcctcgac atctttctcg
     2281 aaacgcgcga gtcgtgggtc gcgtggtcgg cgctgtacag cttctacgcg ctggcgttgc
     2341 tggccatcgc c
//
LOCUS       DASUGA010000296         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_924001, whole genome shotgun sequence.
ACCESSION   DASUGA010000296 DASUGA010000000
VERSION     DASUGA010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924001"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VFX21_05845"
     CDS             <1..864
                     /locus_tag="VFX21_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX5095514.1"
                     /translation="VMQRLAAEPGLEAWLDRTELIAGTSTGGLIALSIAAGISLAELR
                     DLYATRAGIVFQDSFLDDLRDVGKVFGADYDVGNLESEARRVLGDRTLAQLTKRVLVP
                     AFDLDNGATTDRRWKAKLFHNFPGPDSDGDALAYRVAVATAAAPTYFASYDGYVDGGV
                     FATNPSMCALAQTQDARLAAAERADLTELRLISFGTGRTLRYVEGDRLDWGYWQWVRQ
                     LLDVMLDGVNGIADYQCTQLLGDHYHRIAPTFPPAKSIGMDAVDQIPYLIEFANGLDL
                     STSADWLRANW"
     gene            894..1328
                     /locus_tag="VFX21_05850"
     CDS             894..1328
                     /locus_tag="VFX21_05850"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5095515.1"
                     /translation="MADAAPPTPSRPPSLPLILARELASNLATPMFVLNQDGLLVYFN
                     DAAERIIGKPFAEIGMVDAMEFGKLLDLTDLDGNPISRPDSPSGIAFFRRRPSHQVLL
                     ATGFDGVRRTVQATAYPLFGTTEELHGVVTVFWEGTPGDEAE"
     gene            1328..2194
                     /locus_tag="VFX21_05855"
     CDS             1328..2194
                     /locus_tag="VFX21_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5095516.1"
                     /translation="MQTRIWGCRGSVAAPGEDTVRYGGNTSCLEARLSSGHVLVFDAG
                     TGMRPLGAALDLSDVTELHIMLTHLHLDHLQGLGFFKPLFQPGLDVHLWGPPSPVQPL
                     AERIAIYLSPPLFPVRLADLPAKVTFHDAPEEPIEIGSAIVRAAKVTHQGPTVGYRVE
                     EDGRGFAYVPDHEPAIGGRLVEQPDDWISGYDLVRDVDVLFHDAQYLDTEYPNHIGWG
                     HSAIGDALELARRARVGRLVLFHHDPYHTDDDLEAMLADAQRTHATPDGWVSLAHEGM
                     EVVFDTSVVPTA"
BASE COUNT          349 a          825 c          735 g          355 t
ORIGIN      
        1 gtcatgcaac ggctcgcggc cgagcccgga ctcgaggcgt ggctcgatcg aaccgagctg
       61 atcgccggca cgtcgaccgg cgggctcatc gcactctcca ttgcagccgg catctcactc
      121 gccgagctgc gcgacctcta cgcgacgcgc gccggcatcg tgttccagga cagcttcctc
      181 gacgatcttc gtgacgtcgg caaggtgttc ggcgccgact acgacgtcgg caacctggag
      241 tcagaagctc gtcgcgtgct cggcgatcgc acgctcgcgc agctgacgaa gcgcgtgctc
      301 gtgcccgcgt tcgacctcga caacggcgcc accaccgacc gacgatggaa ggcgaagctg
      361 ttccacaact ttcccggtcc cgacagcgac ggcgacgcgc tcgcgtaccg cgtcgcggtt
      421 gccaccgcgg cggcgccgac gtacttcgcg tcatacgacg gctacgtcga cggcggcgtc
      481 ttcgcgacga acccgagcat gtgcgcgctc gcgcagacgc aggacgcgcg ccttgctgcc
      541 gccgagcgcg ccgacctcac ggagctgcgg ctgatctcgt tcggcaccgg tcgcacgctg
      601 cgctacgtcg aaggagatcg gctcgattgg ggctactggc aatgggtacg tcagctgctc
      661 gacgtcatgc tcgacggcgt gaacggcatc gccgactacc agtgcacgca gctcctcggc
      721 gatcactacc accgaatcgc gccgacgttc ccgcccgcga agtcgatcgg catggacgcc
      781 gtcgaccaga tcccgtatct gatcgagttc gcgaacgggc tcgacctctc gaccagcgcc
      841 gattggctgc gcgcaaactg gtgaccccgc gtccggcctc ttaggctgcc gctgtggccg
      901 acgctgcccc cccgacacca tcgaggccgc cgagcctgcc gctgatcctc gcgcgcgagc
      961 tcgcgtccaa cctcgcgacg ccgatgttcg tgctcaacca ggacggcctc ctcgtgtact
     1021 tcaacgatgc ggccgagcgg atcatcggta agccgttcgc cgagatcggc atggtcgacg
     1081 caatggagtt cggcaagctg ctcgatctca cggacctcga cggcaatccg atcagtcgac
     1141 ccgacagccc gtcgggcatc gcgttcttcc ggcgtcgacc ctcacaccag gtgctgctcg
     1201 cgaccgggtt cgacggcgtg cgccgcacgg tgcaggccac cgcctacccg ctcttcggca
     1261 cgaccgagga gctgcacggt gtggtgaccg tgttctggga aggcacgccc ggcgacgagg
     1321 ccgagtgatg cagacgcgga tctgggggtg ccgcgggtcg gtggcggcgc ccggcgagga
     1381 caccgtccgc tacggcggca acacgtcgtg cctcgaggcg cgcctctcga gcggtcacgt
     1441 gctcgtgttc gatgccggca ccggcatgcg tccgctcggc gccgcgctcg acctctccga
     1501 cgtcaccgag ctgcacatca tgctcacgca cctgcacctc gaccacctgc agggcctcgg
     1561 gttcttcaag cccctgttcc agcccggact cgacgtgcac ctctggggtc cgccgtcgcc
     1621 ggtgcagccg ctcgcggagc ggatcgcgat ctacctgtcg cctccgctgt tcccggttcg
     1681 gctcgccgac ctgccggcaa aggtcacgtt ccacgatgcg ccggaggagc cgatcgagat
     1741 cggttcggcc atcgtgcgcg cggccaaggt gacgcaccag ggtccgacgg tcggataccg
     1801 cgtcgaagag gacggccgcg gcttcgccta cgtgccggac cacgagccgg cgatcggcgg
     1861 ccgcctcgtg gagcagcccg acgactggat cagcggctac gacctcgtgc gcgacgtcga
     1921 cgtgctgttc cacgacgcgc agtacctcga caccgagtac ccgaaccaca tcgggtgggg
     1981 gcactccgcg atcggcgacg cgctcgagct cgcacgccgc gcgcgcgtcg ggcggctcgt
     2041 gctcttccac cacgacccgt accacaccga cgacgacctc gaagcaatgt tggccgacgc
     2101 gcagcgcacg cacgcgacgc ccgacggctg ggtgtcactc gcccacgagg gcatggaagt
     2161 ggtgttcgac acctcggtgg ttcccactgc gtgagtctga cctgacatga ccacggtgcg
     2221 gccgacgacg cggtacgtga cggtcgatgg ctcacgcgtc gcgt
//
LOCUS       DASUGA010000297         4429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393 k141_7025,
            whole genome shotgun sequence.
ACCESSION   DASUGA010000297 DASUGA010000000
VERSION     DASUGA010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7025"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFX21_05860"
     CDS             complement(<1..372)
                     /locus_tag="VFX21_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095517.1"
                     /translation="MLSVSCNAGDAKFGDGGFVVGDVGAAAAADVGALVAASLVGVGA
                     VVVVVPEGRVVVVRAVMRGRNAPKPVADCGSTIGVAGAVGPPWFAGGAFVPRGAVVGR
                     SGGCVAGGAVDAGAVVGGAVVG"
     gene            353..490
                     /locus_tag="VFX21_05865"
     CDS             353..490
                     /locus_tag="VFX21_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095518.1"
                     /translation="MQLTDSKESPGTGRNLAAIGVFAVIVAVILLFVRAAGRRSASPA
                     G"
     gene            complement(498..2297)
                     /locus_tag="VFX21_05870"
     CDS             complement(498..2297)
                     /locus_tag="VFX21_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5095519.1"
                     /translation="MRGGGHWSDLVPDETLSRDEAQRLLRRLFRMLRPYRFQMCVVIG
                     VLVAQTATLLAGPALVKHGIDAGLDEGDAGALNLSAGLYLVMAVLGYFLGRWAIIGVA
                     KIGEAFLRSLRRRLFDHLMSLSLDYFEKEKTGRIVARMTSDIDSLQELISQGLVLFVQ
                     NILLFGGAVVVILLMSWQLALGVLIIVPPVYFASRWFRRVSNKAYLEVRDRIATNLST
                     LQESLEGVRVVQAYGREGSFTQQFDETNEGQYEANMETVRISSKYFPIVEFVGVLGTA
                     VIIGLGGYLTSVDVVTVGTVAAFVLYLNNLFEPVQQLSQLYNTVQSAGAALSKIYTVL
                     DTKPSVQERPGAVDLPRAGRIDVESVSFAYVEETVLRDVTLHIAAGERIAFVGPTGAG
                     KSTLAKLIARFYDPGEGSITVDGVDLRNATIKSLRERIVVVPQEGFLFAGTLRDNVRV
                     GRPEATDDEVDDALRVLGMYERFRAFPDGLDTQVRERGSRLSAGERQLVSLARAALAD
                     PAVLVLDEATSNLDPGTEHQVEEALEHLMEGRTVVVVAHRLSTAARADRIAVVYDGRL
                     AELGTHAELIAHEGHYAALYRSWSAHHPDTNVA"
     gene            complement(2294..3994)
                     /locus_tag="VFX21_05875"
     CDS             complement(2294..3994)
                     /locus_tag="VFX21_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5095520.1"
                     /translation="MRPLRGLVVLGILAGLVWTVARVAIPSLTRVAIDNGVAEGNATV
                     ALQWTLLILFAGAVQAVCTGLRRFAAFKLALRVETNLRMRLVAHLQRLHFAFHDHAQT
                     GQLMAHANTDIQQIHQVVLLIPLTIASTLTMLAVIVILVLRSPGLAFFALAALPLLNV
                     AATRFTHRMFPVGLRLQEELADLSGVVEESVSGVRVVKGFGAERMQERKLVTESEDVY
                     DQSIAQARLRANFLPLIDLLPTLGLVGILWYGGHQVLDGNLTVGDIVAANIYVVMLIW
                     PLRMVGMLLGQLSRAAASAGRIHAVLDTDAEIDDAPDAVALGDGPGTVSFRGVTFGYG
                     TRPVLEGLDLEIPGGQAIALVGATASGKSTVARLIPRFYDVSAGQIAIDGHDVRATKL
                     HDVRGAVGLVFEDTFLFSDSVRANIAFADPNASNDRVKRAAVLAGADEFIQDLPEGYE
                     TIVGEHGYSLSGGQRQRIAIARAVLADPRILILDDATSSVDPTKEHEIRNALREVMAG
                     RTTIIIAHRPATIALADRVVLLDGGRIVADGTHESLLASNARYREVLARAEASVGDAA
                     "
     gene            4143..>4429
                     /locus_tag="VFX21_05880"
     CDS             4143..>4429
                     /locus_tag="VFX21_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095521.1"
                     /translation="MPRSLRRCWRASARAEVLVIGMLAAGFAGSGLVARPVVTSPNSE
                     TPATQARVTANAPLQSHADAVRRERWLEAAYDTAQEEKLQQAVDQQRAEHAA"
BASE COUNT          764 a         1537 c         1484 g          644 t
ORIGIN      
        1 gccgacgacc gcgccgccga cgactgcgcc ggcgtcgacc gccccgcccg cgacgcaacc
       61 gcccgaacgg ccgacgaccg cgccacgcgg cacgaacgcg ccgccggcga accagggcgg
      121 gccgaccgcg cccgcgacgc caatcgtcga gccgcaatcc gcgaccggct tcggggcatt
      181 tctgccgcgc atcacggccc gcacgacgac gacgcggcct tccggtacga cgaccacgac
      241 cgcgccgaca ccgacgagcg aggccgcgac gagcgcgccg acgtctgccg cggccgcggc
      301 accgacatca ccgacgacga acccgccgtc gccgaacttc gcgtcccccg cgttgcaact
      361 gacggacagc aaggaatcgc ccggcaccgg ccgcaacctt gccgcgatcg gcgtgttcgc
      421 cgtcatcgtc gcggtgatcc tgttgttcgt gcgtgccgcc ggccgccgtt cggcgtcacc
      481 cgccggctaa cgcgggatca ggcgacgttg gtgtcgggat ggtgcgccga ccaggaccgg
      541 tacagcgcgg cgtagtgccc ctcgtgcgcg atcagctccg cgtgggtacc gagctcggcc
      601 aagcgaccgt cgtacacgac cgcgatgcga tcggcgcgcg ccgcggtcga gaggcgatgg
      661 gcgacgacca ccaccgtgcg tccctccatc aggtgttcga gcgcttcctc gacctgatgc
      721 tcggtgccgg gatcgaggtt cgatgtcgct tcgtcgagca cgaggaccgc gggatccgcg
      781 agcgcagcgc gtgccaacga caccaactgt cgttcgccgg ccgagagccg cgacccgcgc
      841 tcgcgcacct gcgtgtcgag cccgtcgggg aacgcgcgga accgttcgta catgccgagg
      901 acgcgcagcg cgtcgtcgac ttcgtcgtcg gtggcttccg gccgtccgac gcggacgttg
      961 tcgcgcagcg ttccggcgaa caggaagcct tcctgcggca cgacgacgat gcgttctcgt
     1021 agcgacttga tcgtcgcgtt gcgcaagtcg acgccgtcga cagtgatcga tccctcgccg
     1081 gggtcgtaga accgtgcgat cagcttggcc agcgtcgact tgccggcgcc cgtcggaccg
     1141 acgaacgcaa tccgctcgcc ggccgcgatg tgcagcgtca cgtcgcgcag cacggtctcc
     1201 tcgacgtagg cgaacgacac cgactcgacg tcgatgcggc cggcacgcgg caggtcgacg
     1261 gcacccggcc gctcctgcac cgacggcttc gtgtcgagca cggtgtagat cttgctgagc
     1321 gccgcgcccg cggactgcac ggtgttgtag agctgactca gctgctgcac cggctcgaac
     1381 aggttgttca agtagaggac gaacgcggcg acggtgccga cggtgacgac gtcgaccgag
     1441 gtgaggtagc caccgaggcc gatgatgact gcggtgccga gcaccccgac gaactcgacg
     1501 atcgggaagt acttcgacga gatgcgaacc gtctccatgt tcgcttcgta ctgaccctcg
     1561 ttggtctcgt cgaactgctg cgtgaaggag ccttcgcgac cgtaggcctg cacgactcgc
     1621 acgccttcga ggctctcctg caacgtgctc aggttcgtgg cgatgcggtc gcgcacctcg
     1681 aggtacgcct tgttcgacac gcggcggaac caacggctcg cgaagtacac gggcggcacg
     1741 atgatgagca cgccgagcgc gagctgccac gacatcagca agatgacgac gaccgcgccg
     1801 ccgaacagca agatgttctg gacgaagagc acgaggccct gcgagatcag ctcctgcaac
     1861 gagtcgatgt cggaggtcat gcgcgcgacg atgcggcccg tcttctcctt ctcgaagtag
     1921 tcgagcgaca acgacatcag gtggtcgaac aggcggcggc gcaacgaacg caggaacgcc
     1981 tcgccgatct tcgccacacc gatgatcgcc caacggccga ggaagtagcc gaggaccgcc
     2041 atcaccaggt agaggccggc cgacaggttc aacgcgcccg cgtcgccttc gtcgagcccc
     2101 gcgtcgatgc cgtgcttcac gagcgcgggc ccggcgagca gcgttgccgt ctgcgcgacg
     2161 aggaccccga tcacgacgca catctggaac cggtagggcc gcagcatgcg gaacagacgg
     2221 cgcagcaagc gctgcgcctc gtcgcgcgac agcgtctcgt cggggacgag atcggaccag
     2281 tgcccgccgc ccctcatgcc gcatcaccca cgctggcctc ggcccgggcc agcacctcgc
     2341 ggtagcgggc gttcgacgcg agcagggact cgtgcgtgcc gtcggcgacg atgcgcccac
     2401 cgtcgagcag gacaacccgg tccgcgagcg cgatcgtcgc cgggcggtgg gcgatgatga
     2461 tcgtcgtccg cccggccatg acctcgcgca acgcgttgcg gatctcgtgt tctttcgtcg
     2521 ggtcgacaga cgacgtcgcg tcgtcgagga tcaagatgcg cggatccgcg agcacggcgc
     2581 gggcgatcgc gatgcgctgg cgttgcccgc ccgacaacga gtagccgtgc tcgccgacga
     2641 tcgtctcgta gccctcggga aggtcttgga tgaactcgtc ggcgcccgcc aacaccgccg
     2701 cacgcttcac gcggtcgttc gacgcgttgg gatccgcgaa cgcgatgttg gcgcgaaccg
     2761 aatcggagaa gaggaacgtg tcctcgaaga cgagaccgac cgcgccgcgc acgtcgtgca
     2821 gcttcgtcgc gcgaacatcg tggccgtcga tcgcgatctg acccgcggag acgtcgtaga
     2881 agcgcgggat gagccgtgcg accgtcgact tcccggatgc ggtcgcaccc acaagtgcga
     2941 tcgcctgccc gccggggatc tcgaggtcga gaccttcgag caccggccgc gtgccgtacc
     3001 cgaacgtgac accgcggaac gacaccgtgc cggggccgtc gcccaacgcg accgcgtccg
     3061 gcgcgtcgtc gatctcggcg tccgtgtcga gcacggcatg gatgcgaccc gccgacgccg
     3121 cggcgcgtga cagctgcccg agcagcatgc cgaccatgcg caacggccag atcagcatca
     3181 cgacgtagat gttggcggcg acgatgtcgc cgaccgtgag gttgccgtcg agcacttggt
     3241 gcccgccgta ccagaggatg ccgacgagcc cgagcgtcgg cagcaggtcg atgagcggca
     3301 ggaagttcgc ccgcagccgc gcctgcgcga tcgactggtc gtagacgtct tcgctctcgg
     3361 tcacgagctt gcgctcttgc atgcgctcgg cgccgaagcc cttcacgacc cgcacaccgg
     3421 acacgctctc ctcgacgacg cccgagaggt cggccagctc ttcttgcaag cggaggccga
     3481 ccgggaacat gcgatgcgtg aagcgagtcg ccgcgacgtt gagcaacggc agcgcggcca
     3541 acgcgaagaa cgcgagcccg gggctgcgca gcacgagaat gacgatcacc gcgagcatcg
     3601 tgagcgtgct cgcgatcgtc aacgggatga gcagcacgac ttggtggatc tgctggatgt
     3661 cggtgttcgc gtgcgccatc aactggccgg tctgcgcatg gtcgtgaaac gcgaagtgca
     3721 accgttgcag gtgcgcgacc aaccgcatgc gcaggttcgt ctcgacgcgc aacgcgagct
     3781 tgaacgcggc gaatcgacgc aggcccgtgc acactgcttg gaccgcgccg gcgaacagga
     3841 tgagcaaggt ccattgcagt gcaacggtcg cgttgccttc cgcgacgccg ttgtcgatgg
     3901 cgacacgggt cagtgacggg atcgcgacgc gagcgacagt ccagacgagt ccggcaagga
     3961 tgccgaggac gaccaaacca cgcaagggac gcaccgcgcc cctcatcagc cgccatccgg
     4021 ccttccgcgc cgcgcgtgct tcgtccacca attcccccgt tttcccggtt gccgaggcta
     4081 ccagcgggct gtgacagcct caggagcgtt tttccgatgc gtgatctggg gacgcacgtt
     4141 caatgccccg atctctccgt cgttgttggc gcgcgagcgc acgcgcggaa gtgctcgtca
     4201 tcggcatgtt ggccgccggg ttcgcgggct ccggactcgt tgcccgtccc gttgtcacgt
     4261 caccgaacag tgagacgccc gcaacacaag cgcgcgtcac cgccaacgcg ccgttgcaat
     4321 cacatgccga cgcggtcaga cgcgaacgtt ggttggaagc tgcgtacgac actgcacaag
     4381 aagagaagct gcagcaggcg gtcgatcaac agcgcgcgga gcatgcggc
//
LOCUS       DASUGA010000298         3950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_806620, whole genome shotgun sequence.
ACCESSION   DASUGA010000298 DASUGA010000000
VERSION     DASUGA010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806620"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VFX21_05885"
     CDS             complement(<1..385)
                     /locus_tag="VFX21_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095522.1"
                     /translation="MADLRTTITEVVTGLGMLGFDDVDAALSARPEAMENVEPSVYDG
                     LALAWKEHRHADLFTASLMNGRAFLAARDALRHRTPFVIEWKGAHKAPGDEVIPADLR
                     VDHVYFVSCKYLSRIVVNASPHNLFD"
     gene            complement(395..1801)
                     /locus_tag="VFX21_05890"
     CDS             complement(395..1801)
                     /locus_tag="VFX21_05890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX5095523.1"
                     /translation="MATAKRGTSTSNFGVSKRESHDATAFYERFTAPELSDDDTVVAP
                     YEITDPFVLGDARQMDAITDGSVALVVTSPPYYAGKQYEEELEREGVPSSYREYLELL
                     RDVFAECTKKLEPGGRIAVNVANLGRKPFRNLAADVTRILQDDLKLLLRGEVVWRKGE
                     GAAGNCAWGSFRSPANPVLRDTTERVVIASKGRFDRAKSKQQRERQGLPYVNGINADE
                     FMAATLDVWDIPPESARRVSHPAPFPVELPARLIDLYTYENDLVLDPFMGSGSTLVAA
                     SKLRRRYVGYDLDASYVDIARLRVRDEGTPEPHPLPAVPDLDPGTLDFGENFQARATK
                     EGKAAQALAEQLLGEVGFTIVAKNSRVRGTGVTINFIATDADGVEWYFDVSGAFTSTR
                     GGLLRTDTVWKSIGRAHALAQEKDRRIVFLTSHKPRKGSEGDIALRNAEDIIFDIIEM
                     RDPEDIEKLQKYAAGGNS"
     gene            complement(1873..2439)
                     /locus_tag="VFX21_05895"
     CDS             complement(1873..2439)
                     /locus_tag="VFX21_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX5095524.1"
                     /translation="MRLVALFGLLLLAACGANTSDASHDLARKNIPEQITVTSPLFTN
                     NGSLPRGNTCDGAGTPPEIEWRDLPAKTKSVAVVADDPDAGDKPFVHWIVIGLPPVPG
                     SVPSAAKNVHELDNTGGTRGWTAPCPPAGETHHYRFTVYALRDYVCAGDADDIRVQSC
                     SPPGAPSALNLIAANALTKGTMTGTYRR"
     gene            complement(2436..3080)
                     /locus_tag="VFX21_05900"
     CDS             complement(2436..3080)
                     /locus_tag="VFX21_05900"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5095525.1"
                     /translation="MPAADPSPNPTQRVLILRHGESTWNVEKRWQGWLDAPLTERGEA
                     QAAARARTLAHDGFRPRVIYSSDLGRAARTAEIIGAHVSAPVITDEGFRERHGGEWQG
                     RTGTEIDTDYPGQRDAWRRGELTAPPGGEEDAQVLTRFDIAMTHALAHAGTGLLCVVT
                     HHGMLRVVATRAGIDVHTLIPNLGGFWFDVVEGALTRAEPLDTLPEDDVHAAVE"
     gene            3178..>3950
                     /locus_tag="VFX21_05905"
     CDS             3178..>3950
                     /locus_tag="VFX21_05905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016334219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX5095526.1"
                     /translation="MPTAVIVDAVRTPLGRRNGKLKDVHPVEMAAHTLKALVERNDLD
                     PALVEDVIMGCVMQVGDQGLNIARNAALAAGFPESTVGTTIDRQCGSSQQAAHFAAQG
                     VMAGAYDVVIAAGIENMTRVPMGASVLPGSNPFGISMPARYPGLVPQGISAELISEKW
                     NLSREDNDAFSAQSHQRAAKAREEGRFDREIVPIQAGDELVSQDEGIRPDSTTETLAK
                     LKPAFKEDGVITAGNSSQITDGAAAVLIMTEEKANELGL"
BASE COUNT          619 a         1297 c         1359 g          675 t
ORIGIN      
        1 gatcgaacaa gttgtgcggc gacgcattga cgacgatccg cgacaggtat ttgcagctga
       61 cgaagtagac gtgatcaacg cgcaggtcgg cggggatgac ttcgtcgccg ggcgccttgt
      121 gcgcgccctt ccactcgatc acgaacggcg tgcgatgccg cagcgcgtcg cgagctgcca
      181 gaaaggcccg gccgttcatc aaggaggcgg tgaacagatc ggcgtgccgg tgctctttcc
      241 aggcgagcgc aaggccgtcg tacacggacg gttcgacgtt ctccattgcc tcgggccgcg
      301 cactcaacgc ggcgtcgacg tcgtcgaagc cgagcatgcc gaggccggtc acgacctcgg
      361 tgatcgtggt tcgtagatcc gccatcgtgc tccgttagga gttgccgccg gcggcgtact
      421 tctggagctt ctcgatgtct tcgggatcgc gcatctcgat gatgtcgaag atgatgtctt
      481 cggcattacg cagcgcgatg tcgccttcgc tgcctttgcg gggcttgtgc gatgtcagga
      541 acacgatgcg ccggtccttc tcctgggcca gcgcgtgagc ccgaccgatc gacttccaca
      601 ccgtgtcggt gcggagcaga ccgccgcgcg tactggtgaa cgcgccggaa acgtcgaagt
      661 accactcgac gccgtcggcg tcggtggcga tgaagttgat cgtcacgccg gtgccgcgca
      721 cgcgcgagtt cttggcaacg atcgtgaagc cgacctcgcc gaggagctgt tcggcgagcg
      781 cctgcgcggc cttcccttcc ttggtcgcgc gcgcttggaa gttctcgccg aagtcgagcg
      841 tgccgggatc gaggtcgggg accgcgggca gcggatgggg ttcgggcgtg ccttcgtcgc
      901 ggacgcgcag ccgcgcgatg tcgacgtagg aagcgtcgag gtcgtagccg acgtagcggc
      961 gccgcagctt cgacgccgcg accaacgtgg aacccgaccc catgaacggg tcgagcacaa
     1021 ggtcgttctc gtaggtatag aggtcgatca atcgcgccgg cagctcgacg gggaacggcg
     1081 cggggtggga tacgcggcgc gcgctctcgg gcgggatgtc ccacacgtcg agggtcgcgg
     1141 ccatgaactc gtcggcgttg atgccgttga catacgggag cccctggcgt tcgcgttgct
     1201 gcttgctctt ggcccggtcg aagcgaccct tgcttgcgat tacgacccgt tcagttgtgt
     1261 cgcgcagcac gggattcgcc ggactgcgga acgaacccca tgcgcagttg cccgccgcgc
     1321 cctcgccctt gcgccacacg acttcgccgc gcaacagcag cttcaggtcg tcctgcaaga
     1381 tgcgcgtgac gtcggcggcg aggttgcgga acggcttgcg cccgaggttc gcgacgttga
     1441 ccgcgatgcg gccacccggc tcgagcttct tggtgcactc ggcgaagacg tcgcgcaaga
     1501 gttcgagata ctcacggtag gaactcggca cgccttcccg ttcgagttct tcttcgtatt
     1561 gttttccggc gtagtaaggc ggtgaggtga cgaccagcgc aaccgaacca tcggtgatcg
     1621 catccatctg gcgcgcgtcg ccgagcacga acggatcagt gatctcatac ggcgcgacga
     1681 cggtgtcgtc gtcggagagc tcgggcgcgg tgaagcgctc gtagaacgcg gtggcgtcgt
     1741 ggctctcgcg tttggagaca ccgaagttcg aggtgctcgt tccccgcttt gccgtcgcca
     1801 tcaggggcag cctacgcggg gggtgcgcca cggtccggct ctcgcgcgtg cgcgggccgg
     1861 gccgcgcgct gatcagcgcc gataggtccc ggtcatggtt cccttggtca gcgcattggc
     1921 cgcgatcagg ttcaacgcgc tgggcgcgcc gggcggggag cagctctgga cgcggatgtc
     1981 gtcggcgtcg ccggcgcaca cgtagtcgcg caacgcgtac acggtgaacc ggtagtgatg
     2041 ggtctcgccc gcaggcggac acggcgcggt ccatccacgc gtgccgccgg tgttgtcgag
     2101 ttcgtgcacg ttcttcgcgg ccgacggcac cgaaccgggc acgggcggca gcccgatgac
     2161 gatccagtgc acgaacggtt tgtcgcccgc gtcaggatcg tcggccacca ccgcgacgct
     2221 cttcgtcttc gccggcaagt cgcgccactc gatttccggt ggcgtgccgg caccgtcgca
     2281 cgtgttgcct ctgggaagcg agccgttgtt cgtgaacagg ggactcgtca cggtgatctg
     2341 ctcgggaatg ttcttgcgtg cgaggtcgtg cgacgcgtcc gacgtgttcg cgccgcaggc
     2401 ggcgagcaac aacagtccga agagtgcgac gagtctcatt cgaccgcggc gtgcacgtcg
     2461 tcttcgggca gggtgtcgag cggttccgcg cgcgtgagcg caccctcgac cacgtcgaac
     2521 cagaacccgc cgaggttcgg aatgagcgtg tgcacgtcga tcccggcgcg agtcgcaacg
     2581 acgcgcagca tcccgtggtg ggtcacgacg cacaagagac ccgtgcccgc gtgcgcgagc
     2641 gcgtgcgtca tcgcgatgtc gaagcgcgtg agcacctgcg cgtcttcttc gccgccgggc
     2701 ggcgcagtga gttcgccgcg gcgccacgca tcgcgctggc ccgggtagtc ggtgtcgatc
     2761 tccgtcccgg tccggccctg ccattcgccg ccgtggcgct ctcgaaaccc ttcgtcggtg
     2821 atgaccggcg cactcacgtg ggcaccgatg atctcggccg tgcgcgcggc ccggccgagg
     2881 tcagagctgt agatgacgcg cgggcggaac ccgtcgtgcg cgagggtgcg ggctcgggct
     2941 gcggcctggg cctcgccccg ctcggtcaat ggcgcgtcca gccagccctg ccagcgcttc
     3001 tcgacgttcc acgtcgactc gccgtgccgc agaatcagca cgcgctgcgt cgggttgggg
     3061 ctcgggtcgg cggccggcat tggaatgaga gtcaagcaga gcgggtttga attctcgccc
     3121 cgcccccggc aaacttgact gtccggtcaa gttcgcattt ctcccctgga ggacgccatg
     3181 ccgacggctg tcatcgtcga cgcagtacgc accccactcg gacgccgcaa cggcaagctc
     3241 aaggacgtcc acccggtcga aatggccgcc cacacgctga aggcgctcgt cgagcgcaac
     3301 gacctcgacc ccgcgctcgt tgaagacgtg atcatgggct gcgtcatgca ggtcggggat
     3361 cagggcctca acatcgcccg caacgccgcg ctcgcggccg gcttccccga gtcgaccgtc
     3421 ggcacgacga tcgaccgcca atgcggttcg tcgcagcagg ccgcgcactt cgccgcgcag
     3481 ggcgtgatgg ccggcgcgta cgacgtcgtg atcgcggcgg gcatcgagaa catgacgcgc
     3541 gtgccgatgg gcgcttcggt gctccccggc agcaacccgt tcggcatctc gatgccggcg
     3601 cgctaccccg gcctcgtgcc gcagggcatc agcgcggaac tcatctccga gaagtggaac
     3661 ctctcgcgtg aggacaacga cgcgttctcg gcgcagtcgc accagcgcgc cgccaaggct
     3721 cgtgaagaag gccgtttcga ccgcgagatc gtgccgatcc aggccggcga cgaactcgtg
     3781 tcgcaggacg aaggcattcg tcccgactcc acaaccgaga cgctcgccaa actgaagccg
     3841 gcgttcaagg aagacggcgt catcaccgcc ggcaactcgt cgcagatcac cgacggtgcg
     3901 gcggcagtgt tgatcatgac cgaggagaag gcgaacgagc tcggactcaa
//
LOCUS       DASUGA010000299         2224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2004035, whole genome shotgun sequence.
ACCESSION   DASUGA010000299 DASUGA010000000
VERSION     DASUGA010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2004035"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..>2224
                     /locus_tag="VFX21_05910"
     CDS             10..>2224
                     /locus_tag="VFX21_05910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HEX5095527.1"
                     /translation="MTALDDFVAQYERPLRRVVIPIYFGLAIVAEQALVDRHPSLAAE
                     LDRLESALGKDALLELAEQTRLRALVHQRKEVGRRETLLEASAVRYLALLKRTLLDEV
                     YIENEVRLQYLADCITKNRPVDETQLRDPVRYLKQQTAGLESVRATGALGDAAGTGAS
                     FLPYTDMGRARLDHLEHALDVIRREDISGDLVDCSTGRGGGGIFMRGYLDAYQVPDRL
                     VWVVDRFRVHARGAGSLDLGTDLNQVRDAFARFDVFDDRVRFVQGDYGETLKGTATGA
                     IALLRIGADSDDAGAILERLYPRLAAGGFVVVDDYASPACRARVDEYRAARGLTEPLD
                     RIDASAVSWRKATSAPGAAPRAVEPRSRQTTVDLSVVVVFYNMRREAARTLHSLSRAY
                     QEGLDGVAYEVIVVENGSAPDQKLGADYVTSFGPEFRYVDLDDAARPSPVYALNHGAA
                     LATGNAVAFMIDGAHVLTPGVLRNGLLGLSAYEPSVVVTQQWYVGPGQQGDEMRTGYD
                     QAFEDKLFDRIGWPSDGYRLFEIGHFIGDRDWLDGLWESNCIFATRALLDQVGVFDEA
                     FSVAGGEYANLDFYERLVANPRTTMVSILGEGSFHQVHGGTTTNQLDTDGRRARIVSY
                     AERYKDLRGRNYKGPGKTIHYIGSMRASALRTRARRMTGTAFRDARVDADATVSVPMP
                     DQLRDEFIDAFWHTQAWRGTEWLGVTVERPASDLVAYQTVVARTRPDWIVVTGPRS"
BASE COUNT          355 a          771 c          737 g          361 t
ORIGIN      
        1 aacggtgtca tgaccgcgct cgacgacttc gtcgcgcaat acgaacgacc gctgcggcgc
       61 gtcgtgatcc cgatctactt cgggctcgcg atcgtcgcgg agcaggcgct cgtcgaccgt
      121 catccgtcgc tcgcggccga gctcgaccgc ctcgaaagtg ctctcggcaa ggacgcgttg
      181 ctcgagctcg ccgagcagac gcgactgcgc gcactcgtgc accagcgaaa ggaggtcggg
      241 cggcgcgaga cgttgctcga ggcgagcgcc gtccgctacc tcgcgctgct caagcgcaca
      301 ctcctcgacg aggtctacat cgagaacgag gtgcggttgc aatatctcgc cgactgcatc
      361 accaagaacc gcccggtcga cgaaacacag ctccgcgacc ccgtccggta cctgaagcag
      421 cagaccgcgg ggttggagag cgtgcgggcg accggcgccc tcggcgacgc ggcgggcacc
      481 ggtgcctcgt tcttgccgta caccgacatg gggcgggcgc gtctcgatca cctcgaacac
      541 gcgctcgacg tgatccgccg cgaggacatc tccggcgacc tcgtcgactg cagcaccgga
      601 cgcggcggcg gcggcatctt catgcgcggc tacctcgacg cctaccaagt ccccgaccgg
      661 ctcgtctggg tcgtcgatcg cttccgcgtg cacgcgcgtg gcgccggatc gctcgatctc
      721 ggcaccgatc tcaaccaggt ccgggacgcc ttcgcgcgat tcgacgtctt cgacgatcgg
      781 gttcgcttcg tccaaggcga ctacggcgag acactgaagg gcaccgcgac cggcgcgatc
      841 gcgttgctgc ggatcggcgc cgactccgac gacgccggcg cgatcctcga gcgcctctac
      901 ccgcgcttgg cggcgggcgg tttcgtcgtc gtcgacgact acgcgtcgcc tgcttgccgc
      961 gcgcgcgtcg acgaataccg cgccgcgcgc gggctgaccg aaccgctcga ccgcatcgac
     1021 gcgtcggcag tgtcgtggcg caaggcgacg agcgcgccgg gcgccgcacc acgcgccgtc
     1081 gagccgagaa gcaggcagac gacggtcgat ctctcggtcg tcgtcgtctt ctacaacatg
     1141 cgccgtgagg cggctcgcac gctgcattcg ctgtctcgcg cctaccaaga aggacttgac
     1201 ggcgttgcgt acgaggtcat cgtcgtcgag aacggctccg caccggacca gaagctcggc
     1261 gcggactatg tcaccagctt cggtcccgag tttcgttacg tcgatctgga cgacgccgcg
     1321 cgtccctctc cggtctacgc gctgaatcac ggtgctgcat tggcgaccgg gaatgccgtc
     1381 gcgttcatga tcgacggcgc acacgtgctg acgccgggtg tgctccgcaa cggactgctc
     1441 ggtctcagcg cctatgagcc ctcggtcgtc gtcacacagc agtggtacgt cggaccgggt
     1501 caacagggcg acgagatgcg caccggatac gaccaggcgt tcgaagacaa gctgttcgac
     1561 cgcatcggtt ggcccagcga cggctaccgg ctgttcgaga tcggccactt catcggcgat
     1621 cgcgactggc tcgacgggct gtgggagagc aactgcatct ttgccacgcg cgcgctgctc
     1681 gaccaggtgg gcgtgttcga cgaggccttt tcggtggccg gcggtgagta tgccaacctc
     1741 gacttctacg aacggctcgt cgccaacccc cgaacgacga tggtgtcgat cctcggcgag
     1801 ggttcgttcc atcaagtgca tggcggtacg acgacgaacc agctcgatac cgacggacgc
     1861 cgcgcgcgca tcgtctcgta tgccgagcgc tacaaggact tgcgcggccg gaactacaag
     1921 ggccccggca agacgatcca ctacatcggg tcgatgcggg cgtctgcgct gcggactcgt
     1981 gcccgccgga tgaccggtac cgcgttccgc gacgcgcgcg tcgacgccga cgcgaccgtg
     2041 tccgtgccga tgccggatca gcttcgtgac gagttcatcg acgcgttctg gcacacgcag
     2101 gcgtggcgcg gcacggagtg gctcggtgtc accgtcgaac gcccggcgtc agatctcgtc
     2161 gcgtaccaga ccgtggtggc gcggacccgg ccggactgga tcgtcgtcac cggcccgcgc
     2221 agcg
//
LOCUS       DASUGA010000300         2858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1169984, whole genome shotgun sequence.
ACCESSION   DASUGA010000300 DASUGA010000000
VERSION     DASUGA010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169984"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..2245
                     /locus_tag="VFX21_05915"
     CDS             206..2245
                     /locus_tag="VFX21_05915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEX5095528.1"
                     /translation="MSHRKLGTWAVAILLASACDCGGGDDPPPGGDGGPDGGGIVPTD
                     APPPVDAQVDGGASVCGDGMMDGPELCDDGNTDDGDGCRGDCLSDYRCGNGVTDTIAS
                     GGTVDELCDDGNTVNGDGCNATCDSDESCGNGIVDLGAGEVCDDGNTMGGDDCSADCL
                     VSLLCGNGAIDPPEECDDGDTTSGDGCSAACRIERCGNGITDAGEECDDGNADDTDGC
                     TASCEFTCAADADCDDGNVCSGTETCTDPGTATSGCAAGTPAADGTSCGGGRVCMSGV
                     CTGVGCGDGTVSGTEQCDDGNLADGDGCDSDCTWTCVSAASCSDGNPCNGNERCAMPS
                     TLMSRCLPRAAPADGTVCDRDMMAGTRDICLMMTCVRSRCGDGFTDPGGMPVEQCDDG
                     NTTSGDGCSSTCMTEMAVPPTAFRVTNIDLISPRIVVVPFIGCNDVTQTPVAGFSVNG
                     ALDTAVRPMSAGGDYNLHIVNLFRPLAPASASTPVELHLNAMCMEAPTPDSCGPDPMP
                     DTILSNANNMSAGTCFTPVAADVNTRAGTPAAYTPTANTVSAPCFVSDEETLTISVAG
                     IMIPLQRARVAATYSGTPTNRLVTGVVTGFLSETAAADILLPSTLPVVGGDPLYSHLQ
                     AGNRSVMNSAGMSIPDGCNVGGGTSEDDADTLPGGTRGFWFFLNFEADLITWTGP"
     gene            complement(2258..>2858)
                     /gene="map"
                     /locus_tag="VFX21_05920"
     CDS             complement(2258..>2858)
                     /gene="map"
                     /locus_tag="VFX21_05920"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX5095529.1"
                     /translation="EGARSAPRLAYDFPGNTCISVNDALAHGIPSAAVVLREGDLVNV
                     DVSAELDGFWADTGASAAVGEVSARHRTLLDVTRVARDEAIATVRAGARVGVVGRAVE
                     RRAKRHGFRVIRNIGGHGVGRYIHEEPHVANHFDPSARTVLHEGLVLAIEPFLTTGAT
                     QVIEDADGWTLRTPDGSRGAQFEHTVVVTRGAPIILTAA"
BASE COUNT          462 a         1016 c         1014 g          366 t
ORIGIN      
        1 cacacgccgc caagaacagc gccgcgaacc cgaatgaaat cgtgcttccg tgaaccatcg
       61 atcacctccg gcacggccca tcgccgcacc cgagaaggag tacgcagcgg atcttgcgaa
      121 gcggttgagg atggcccggc cccgaccctg gttaccccct cggacgaccc atgtagactc
      181 tggtcatacc gaacgaggtg gggccatgag ccatcgcaag cttggaactt gggccgtcgc
      241 gattcttctc gcgagcgcgt gtgattgcgg gggcggggac gacccgccgc caggcggcga
      301 tggcgggccg gacggcggtg ggatcgttcc gaccgatgcg ccgccgccgg tcgacgcgca
      361 ggtcgacggt ggggcgtcgg tgtgcggcga cgggatgatg gacggccccg agctctgcga
      421 cgacggcaac accgacgacg gcgacgggtg tcgcggcgac tgcctgtcgg actaccgctg
      481 cggcaacggc gtgacggaca cgatcgcgag cggcggaacg gtcgacgagc tctgcgacga
      541 cggcaacacc gtcaacggcg acggctgcaa cgcgacgtgc gactcggacg agagctgcgg
      601 caacggcatc gtggatctcg gcgcgggcga ggtgtgcgac gacggcaaca cgatgggcgg
      661 cgacgactgc agcgcggact gcctcgtgag cctgctctgc ggcaacggcg cgatcgaccc
      721 gccggaggag tgcgacgacg gcgacacgac gtccggcgac gggtgcagcg ccgcgtgccg
      781 catcgagagg tgcggaaacg gcatcaccga cgcgggcgaa gagtgcgacg acggcaacgc
      841 cgacgacacc gacggctgca ccgcgagctg cgagttcacc tgcgcggcgg acgcggactg
      901 cgacgacggc aacgtctgca gcgggaccga gacgtgcacg gaccccggca cggcgaccag
      961 cggctgcgcg gcgggcacgc ccgcagcaga cggaacgagc tgcggcggcg ggcgcgtgtg
     1021 catgtcgggc gtgtgcacgg gagtcggctg cggcgacgga accgtctccg gcaccgagca
     1081 gtgcgacgac gggaacctcg ccgacggcga cgggtgcgac agcgactgca cgtggacgtg
     1141 cgtgagcgca gcgagctgct ccgacggcaa cccctgcaac ggcaacgagc gctgcgccat
     1201 gccgagcacg ctcatgagcc gttgcctgcc gcgcgcggcg ccggcggacg gaaccgtgtg
     1261 cgaccgcgac atgatggcgg gcacccgcga catctgtctg atgatgacgt gcgtgcgctc
     1321 gcggtgcggt gacggcttca ccgaccccgg cgggatgccc gtcgagcaat gcgacgatgg
     1381 caacacgacg agcggcgacg gatgcagctc gacgtgcatg accgagatgg cggtgccgcc
     1441 gacggcgttc cgcgtgacga acatcgacct catctcgccg cgcatcgtcg tcgtgccctt
     1501 catcggctgc aacgacgtga cgcagacgcc ggtcgcgggg ttctccgtca acggcgcgct
     1561 cgacacggcc gttcggccga tgtcggcggg cggggactac aacctccaca tcgtgaacct
     1621 cttccgcccg ctcgcgcctg cgagcgcgtc gacccctgtc gagctccacc tgaacgcgat
     1681 gtgcatggag gcaccgacgc ccgattcgtg cggcccagac ccgatgcccg acacgatcct
     1741 gtcgaacgcg aacaacatga gcgcgggcac gtgcttcacg cccgtggccg cggacgtcaa
     1801 cacccgcgcg ggcacgccag cggcctacac gccgacggcg aacaccgtga gcgcgccctg
     1861 tttcgtcagc gacgaggaga cgctcacgat ctcggtcgcc gggatcatga tcccgctcca
     1921 gcgggcccgt gtcgcggcga cctactcggg cacgccgacg aaccgtctcg tgaccggcgt
     1981 cgtgaccggc ttcctctcgg agacggcggc cgccgacatc ctcctgccga gcacgctgcc
     2041 cgtcgtcggc ggcgacccgc tctactcgca cctccaagcc ggcaaccgga gcgtcatgaa
     2101 cagcgcgggc atgtcgatcc ccgacggatg caacgtgggc ggcggcacga gcgaggacga
     2161 cgcagacacg ctccccggcg gcacgcgcgg cttctggttc ttcctgaact tcgaggccga
     2221 cctcatcacg tggacgggcc cgtaggcctc gacgacgtca cgcggccgtc aggatgatcg
     2281 gcgcgccgcg cgtcacgacg acggtatgct cgaactgagc gccgcggctc ccgtcgggcg
     2341 tgcgcagcgt ccagccgtcc gcgtcctcga tgacttgcgt cgcgccggtc gtgaggaacg
     2401 gctcgatcgc gagcacgagg ccctcgtgca acacggtgcg cgcgctcggg tcgaagtggt
     2461 tcgcgacgtg cggctcctcg tggatgtacc gcccgacgcc gtgtccgccg atgttgcgga
     2521 tcacgcggaa accgtggcgc ttcgcgcgtc gctcgacggc gcgcccgacg acgccgacgc
     2581 gcgcgcctgc ccgcaccgtc gcgatcgcct cgtcgcgcgc gacgcgcgtc acgtcgagca
     2641 gtgtgcgatg gcgcgccgac acctcgccga ccgccgcgct cgcgcccgtg tcggcccaga
     2701 acccgtcgag ctccgccgac acgtcgacgt tcacgaggtc gccctcgcgg agcacaaccg
     2761 ccgccgacgg gatcccgtgc gccagcgcgt cgttcacgct gatgcaggtg ttccccggaa
     2821 agtcgtacgc gagccgaggc gccgaccgcg cgccttcg
//
LOCUS       DASUGA010000301         4407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393 k141_431,
            whole genome shotgun sequence.
ACCESSION   DASUGA010000301 DASUGA010000000
VERSION     DASUGA010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VFX21_05925"
     CDS             complement(<1..920)
                     /locus_tag="VFX21_05925"
                     /inference="COORDINATES: protein motif:HMM:NF014998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX5095530.1"
                     /translation="MAEARFEHHMSDEDALMWNIEKDPILRSTILAVAEFDRPPDWAA
                     LRTRIDRGTRVIPRLRQRVLSPPLRIGPPRWTVDPSFDLDFHLRRVRLPAPGTERVLL
                     DVLQPIASAAFDRARPLWEFTLLEGLVREDGTEGAALAMKVHHSVTDGVGGMALLTEL
                     VDFERFAPPPDDTNVPAAPAPEAFDSLALIRDSLGHTTRRFLGIARRVPATVLTTTVV
                     AVRHPAAAGVQLAQTARSVARILAPATAPMSPVMRDRGMGRQLAKFTISIDDVRRAGK
                     SVDGSINDVFLAAVTGGLQRYHAHHGTAPP"
     gene            958..1317
                     /locus_tag="VFX21_05930"
     CDS             958..1317
                     /locus_tag="VFX21_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5095531.1"
                     /translation="MKPTEIIANLPVADIGEARSFYTDFLGLDEESFNLGWVAHFRTS
                     DGRAAVQLVTKDASAPVDSAISVRVGDQVDEAYAEAQRRGYEIVHPLTVEEWGIRRFF
                     VRAPDGTVVNIAGHKDE"
     gene            complement(1349..1795)
                     /locus_tag="VFX21_05935"
     CDS             complement(1349..1795)
                     /locus_tag="VFX21_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095532.1"
                     /translation="MLHRAVRGPDGREWAVRREWLKRAPRWPSRRKKQRDPDPVDGVH
                     EAAGCVGEFLIDIPALAIVGLFVLAIVLAFLFVWPAVVFLVDLLVLLLLAAWGVALRA
                     MFKRPWRVLATTDGPPSQTFESHVVGWSASLRERNRLADDITTGRL"
     gene            1840..2724
                     /locus_tag="VFX21_05940"
     CDS             1840..2724
                     /locus_tag="VFX21_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5095533.1"
                     /translation="MRSRYVTTNGIRLFCLEAGPADGPCVLLLHGFPELAYSWRAQVA
                     ALGAAGYHVVAPDLPGYGRSDKPDVTYDSDFINASLVGVVDALGHDQFVVAGHDWGGI
                     LVWIMARKYPERVAGVIGVNTPDLPRTPVPPVEMLRQIFVDTPIYIIQFQERGLAEWV
                     LGTWGRGTDDFIEMIFGATTNADDAFPPEVLDVYKDAFRPQGALTPPIEYYRNMDRNW
                     EINADVADRKVEVPCLMICAADDPVLTPAMSIGMEERVPNLDRVTIENCGHWTQQERP
                     QETTDAMLTYLDKLPRWT"
     gene            complement(2729..3499)
                     /locus_tag="VFX21_05945"
     CDS             complement(2729..3499)
                     /locus_tag="VFX21_05945"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5095534.1"
                     /translation="MSAKLAARVVVVTGGASGLGEAIVSRLASDGDRVVVADVDEPRA
                     QQIADNLVAAGRLAEAATLDVTSEGEVAAMFDAINERHGRLDALVCSAAIETRKSIVD
                     CTDDEWQSVIDVNLKGPFLCMKHGIPMIARQGGAVVLLGSVLGAMGSPGYPAYCASKG
                     ALVNLAKQAAIEHAPDGVRVNVVSPSATDTGLFARMAEASGDPDRIKAMVIAGTPMGR
                     LGRADEVSATVSFLCSPGAAFISGTVIPVDGAMAARRV"
     gene            complement(3496..4266)
                     /locus_tag="VFX21_05950"
     CDS             complement(3496..4266)
                     /locus_tag="VFX21_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5095535.1"
                     /translation="MRGEPLLEGKVALITGAGSGQGRAAALLFAREGASITIAEINLA
                     GAVETAALVEKAGGRALAIHADVSRRADCDRMVEETVAAFGGLHVLYNNAAVQMSGRL
                     VECTEDDWDLTIATNLNAIFYACRAAIPYLQRDGGSIINTASTLGLIGSEGYAAYGAA
                     KAGLLALTRQIAVEYGPAVRANVIAPGSIDTPRFRKVTAQLEDPDSFLRMLSKNTPLS
                     RLGTADDIARVALFLASDLSGYTSGAIVPVDGGLAALR"
BASE COUNT          713 a         1548 c         1492 g          654 t
ORIGIN      
        1 tcgggcggcg ccgtcccgtg atgcgcgtgg taccgctgca ggccgccggt cactgccgcg
       61 aggaacacgt cgttgatgct gccatcgacc gacttgcccg cgcgacgcac gtcgtcgatc
      121 gagatcgtga acttcgcgag ctgacggccc ataccgcggt cgcgcatcac cggtgacatc
      181 ggcgcggtcg cgggcgcaag gattctggcg accgaacgcg ccgtctgcgc gagctgcacg
      241 ccggcggccg cgggatggcg aactgcgacg actgtcgtgg tgagcaccgt cgccggcacc
      301 cggcgcgcga tgccgaggaa gcgtcgcgtc gtgtggccga gcgagtcacg gatcaacgcc
      361 agcgagtcga acgcttcagg cgccggtgcg gcggggacgt tcgtgtcgtc gggcggcggc
      421 gcgaatcgct cgaaatcgac gagctcggtc agcagcgcca tcccgccgac accgtcggtg
      481 acggagtggt gcaccttcat cgcgagcgcg gcgccctcgg tgccgtcttc gcgcacgagt
      541 ccttcgagca gcgtgaactc ccacagcggc cgggcccggt cgaacgcggc cgacgcgatc
      601 ggctgcaaca cgtcgagcag cacccgctcg gtgcccggcg cggggagccg cactcggcgc
      661 aggtggaagt cgaggtcgaa gctcgggtcg accgtccagc gcggcggccc gatgcgcaag
      721 ggcggcgaga gcactcgctg gcgcaggcga ggaatgaccc gggtgccgcg gtcgatgcgc
      781 gtgcgcaacg ccgcccagtc gggcggccgg tcgaactcgg ccaccgccag gatcgtcgag
      841 cggaggatcg ggtccttctc gatgttccac atgagcgcgt cctcgtcgct catgtgatgt
      901 tcgaaccggg cctccgccat cggcgaagaa taatcgttac cgacgcgagg agcgttcgtg
      961 aagccgacgg aaatcatcgc caacctgccg gtcgccgaca tcggcgaggc ccgcagcttc
     1021 tacaccgact ttctcgggct cgacgaggag agcttcaacc tcgggtgggt cgcacacttc
     1081 cgtacttccg acggtcgggc cgccgtacag ctcgtcacca aggacgcaag cgcgccggtc
     1141 gactccgcca tctcggtgcg cgtcggcgac caggtcgacg aggcgtacgc cgaagcgcaa
     1201 cggcgcgggt acgagatcgt gcacccgctg acggtcgagg agtggggcat ccgccggttc
     1261 ttcgtccgtg cgcccgacgg caccgtcgtc aacatcgccg gccacaaaga cgaatgatcg
     1321 ggcgtctctc aagggcgaat tccgcccttc acagacgccc ggtggtgatg tcgtcggcga
     1381 gccggttgcg ctcgcgcaga ctggcgctcc atccgaccac gtgtgactcg aaggtttggg
     1441 acgggggacc gtccgtcgtc gcgaggacgc gccacggtcg cttgaacatc gcgcgcaacg
     1501 ccacgcccca cgccgcgagc agcagcagca cgagaaggtc gacgaggaag accaccgccg
     1561 gccagacgaa caagaacgcg agcacgattg cgagcacgaa gaggccgacg atggccagcg
     1621 ccggaatgtc gatgagaaac tcgccaacgc aacccgcggc ctcatggaca ccatctaccg
     1681 ggtccgggtc tcgttgcttc ttcctccgcg acggccagcg cggcgcgcgc ttcaaccatt
     1741 cccgccggac cgcccactcg cgcccgtccg gcccccgcac cgcccgatga agcacaccgc
     1801 aagtgtgccc gatgtgccgt ccaagttagg ttccttttca tgcgcagtcg ctacgtgacg
     1861 acgaacggga ttcgcctctt ctgcctcgag gcgggtccgg ccgacggacc gtgcgtgttg
     1921 ctgctccacg ggttccccga gctcgcctac tcctggcggg cgcaggtcgc ggcgctcggc
     1981 gccgccggct accacgtcgt cgcgcccgac ctgccgggat acggccgctc cgacaagccc
     2041 gacgtcacct acgacagcga cttcatcaac gcgtcgctcg tcggtgtcgt cgacgcgctc
     2101 gggcacgacc agttcgtcgt cgccggacac gactggggcg gcatcctcgt gtggatcatg
     2161 gcccgcaagt atcccgagcg cgtcgcgggc gtcatcgggg tgaacacacc tgacttgccg
     2221 cgcacgccgg taccgcccgt cgagatgctg cggcagatct tcgtggacac gccgatctac
     2281 atcatccagt tccaagaacg aggtctcgcc gagtgggtgc tcggtacctg ggggcgcggc
     2341 accgacgact tcatcgagat gatcttcggc gcgaccacga acgcggacga cgcgttcccg
     2401 cccgaggtgc tcgacgtgta caaggatgcg ttccgtccgc agggcgcgct cacgccgccg
     2461 atcgagtact accgcaacat ggaccgcaac tgggagatca acgccgacgt cgcggaccgc
     2521 aaggtcgagg tgccgtgctt gatgatctgc gcggccgacg acccggtgct cacaccggcg
     2581 atgtcgatcg ggatggagga acgcgtgccc aacctcgacc gcgtgaccat cgagaactgc
     2641 gggcactgga cccagcaaga acgtccgcag gagacgacgg acgcgatgct gacctacctc
     2701 gacaagctgc cgcgctggac gtagcgccct acacccggcg ggcggccatg gcgccgtcga
     2761 ccgggatcac cgtgccgctg atgaacgcgg cgcccggtga gcagaggaac gacaccgtcg
     2821 cgctgacttc gtcggcgcgt ccgaggcgac ccatcggcgt gcccgcgatg accattgcct
     2881 tgatgcgatc cgggtcaccc gacgcctccg ccatgcgcgc gaacaagccc gtgtccgtcg
     2941 ccgacggcga aacgacgttg acgcgcacgc cgtcgggtgc gtgttcgatc gcggcttgct
     3001 tcgcgaggtt gacgagtgct cccttcgacg cgcagtacgc cggatagccg ggcgatccca
     3061 tcgcgcccag cacggagccg agcaacacga cggcgccacc ctggcgcgcg atcatcggga
     3121 tcccgtgctt catgcacagg aacgggccct tgaggttcac gtcgatcacc gactgccatt
     3181 cgtcgtcggt gcagtcgacg atcgacttcc gagtctcgat cgcggccgag cacacgagcg
     3241 cgtcgaggcg gccgtgccgc tcgttgatcg cgtcgaacat cgccgccacc tcgccctcgc
     3301 tggtgacgtc gagcgtcgcc gcctccgcga gccggccggc ggcgacgagg ttgtcggcga
     3361 tctgttgcgc acgcggttcg tcgacgtccg cgacgacgac ccggtcgccg tcgctcgcca
     3421 accggctcac gatggcttca ccgagcccgg atgcaccgcc ggtgaccacg accacgcggg
     3481 ccgcgagctt ggcgctcatc gcagcgccgc cagcccaccg tcgaccggga cgatcgcacc
     3541 cgacgtgtag cccgacaggt cgctcgcgag gaacagtgcg acccgtgcga tgtcgtcggc
     3601 cgtgccgagc cgcgacagcg gtgtgttctt cgacagcatg cgcaggaagg agtcgggatc
     3661 ctcgagctgt gcggtcacct tgcggaaccg cggcgtgtcg atcgaccccg gcgcgatgac
     3721 gttggcgcgc actgcagggc cgtattcgac ggcgatctgg cgcgtgagcg cgagcagccc
     3781 ggccttggcc gcgccgtacg cggcatagcc ctcggatccg atgaggccga gcgtcgacgc
     3841 ggtgttgatg atcgacccgc cgtcgcgttg caggtacggg atcgcggcgc ggcacgcgta
     3901 gaagatcgcg ttcaggttcg tcgcgatcgt gaggtcccag tcgtcctccg tgcactcgac
     3961 gaggcgaccc gacatctgca ccgcggcgtt gttgtagagc acgtgcaagc caccgaacgc
     4021 ggcgaccgtc tcctcgacca tgcgatcgca gtccgcgcgg cgcgacacgt cggcatgaat
     4081 cgcaagtgcg cgcccacccg ccttctcgac gagcgcggcg gtctcgacag cacccgcgag
     4141 gttgatctcg gcaatcgtga tcgacgcgcc ctcgcgcgcg aagaggagcg cggcggcgcg
     4201 gccctgtccg gatcccgcgc cggtgatcaa tgccaccttg ccttcgagca acggctctcc
     4261 ccgcacgcac cgtacgatac gccgatggtt tcggacgcgg tacaggagca cggcgcgctg
     4321 ttcgaccggc gtggcgagat gttcgtgccg acgccgctcg cgcgcggtcc ttgggatccg
     4381 aacgcgctgc acggcggcgc gccgagc
//
LOCUS       DASUGA010000302         2836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2050895, whole genome shotgun sequence.
ACCESSION   DASUGA010000302 DASUGA010000000
VERSION     DASUGA010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2050895"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VFX21_05955"
     CDS             <1..618
                     /locus_tag="VFX21_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015794529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX5095536.1"
                     /translation="NGAHFEAYTLLAAMAAETQHADIGALVTCNSYRNPHLLADMART
                     IDHISNGRFVLGIGSGWFERDYEEYGYEFGTAPERLRELARALPTIMDRLSRLEPPPR
                     GRLPILVGGSGEKVTLRLVAEHADMWNSFGPPEHFAAKNRVLDEWCARLGRNPRQIER
                     TCAIDGPEVDNWPAYLDAGADHLIVMAGPPFDLDPLRRLLDHARG"
     gene            complement(779..1588)
                     /locus_tag="VFX21_05960"
     CDS             complement(779..1588)
                     /locus_tag="VFX21_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095537.1"
                     /translation="MTASVCALSVLLVLFALGDIYVSPQNDRVDRYGGTGTSRRANGQ
                     PFATFDEPPVQQIFVQAIARRTTSSTTTSTSPTTAPGAPTTTPRGRGGSPVTEPRIVA
                     VTSPSLGSSTTTTTTVPRPAIPPLPSLPPLPPLPVLPVSPSLPTNKTVAPMTIPTTTS
                     TSTSTTTIPPAPVTLDATLSLSVSAIPAGPYDINSDAAPRLSWSTVDATQASVSGLGV
                     VSLDLSGTVTLCPGVVTDNVCTPMANTYDYTLTARDDLGHTVTRTVTLVVG"
     gene            complement(1768..2424)
                     /locus_tag="VFX21_05965"
     CDS             complement(1768..2424)
                     /locus_tag="VFX21_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095538.1"
                     /translation="MRKAVESRRTSALSPIVSIVGGLAALSTLLVMFGLSEVRNATPQ
                     EPRSAGRIAGVVTDRSGPGVDLDAPTTTTVPPELPSVPIPDVRAIAAVVTTPTTAATA
                     PAPPAPVVAEPAAVVVASVQAEAPAVTKKDRGKHRPHRPHGPHQPKDARGSRDAQQAA
                     AAAGALAAAPAAADPVGDGDNRGRQPYKPHTPHQSRRDRADGSSAQEEDCTDNAANDA
                     "
     gene            complement(2511..>2836)
                     /locus_tag="VFX21_05970"
     CDS             complement(2511..>2836)
                     /locus_tag="VFX21_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX5095539.1"
                     /translation="LGNNDNTLVGVLPETVTLDLDGVRVAMVHETGATKGRAARVHRR
                     FPDAAVVVYGHSHQPDDSIGVDGQRLFNPGSPTERRRATNHTYGLLTFDNARVRVHRI
                     VSCDS"
BASE COUNT          418 a          979 c         1002 g          437 t
ORIGIN      
        1 aacggtgcgc acttcgaggc ctacacgctg ctcgccgcga tggccgccga gacacagcac
       61 gccgacatcg gtgcgctcgt cacatgcaac tcgtaccgca acccgcactt gctcgccgac
      121 atggcgcgca cgatcgacca catcagcaac ggtcgcttcg tgctcgggat cgggtccggc
      181 tggttcgagc gcgactacga agagtacggc tacgagttcg gtactgcgcc ggaacgcctg
      241 cgcgagctgg cccgtgccct gccgacgatc atggaccgct tgagccggct cgaaccaccg
      301 ccgcgcggtc ggctgccgat cctcgtcggc ggcagcggcg aaaaggtcac gctgcgactc
      361 gtcgccgagc atgccgacat gtggaactcc ttcggtccgc cggagcactt cgcggccaag
      421 aaccgcgtgc tcgacgagtg gtgtgcacgt ctgggccgca acccgcgtca gatcgagagg
      481 acgtgcgcga tcgacgggcc cgaggtcgac aactggcccg cgtacctcga cgcgggcgcc
      541 gaccacttga tcgtgatggc gggcccgccg ttcgacctcg acccgctacg ccgtctcctg
      601 gaccacgcgc gcggctaaag ggcgcgagaa gccgagcagg cggtggcgtc tcgtcctcgc
      661 gatgcgcgaa gcgcccctga ggaccttctc ggccactcgc ggagtctttc ggcgggccaa
      721 caatccgggc tgcctcacgc gagcacgtcg aaagcgacaa ggccaagtgc ccggcgaatc
      781 agccgacgac gagcgtgacg gtgcgcgtga ctgtgtgccc gaggtcgtcg cgggcggtca
      841 gggtgtagtc gtaggtgttg gccatcggcg tgcacacgtt gtcggtcacc acgccggggc
      901 acagtgtcac cgtgcccgag aggtcgagcg acacgacccc gaggccgctc accgatgcct
      961 gtgtcgcgtc cactgtcgac cacgacaggc gcggtgcggc gtcggagttg atgtcgtagg
     1021 ggccggccgg gatcgcgctg accgacagcg acagcgtcgc gtcgagcgtg accggcgcgg
     1081 gcgggatcgt cgtggtcgac gtcgacgtcg acgtcgtcgt cgggatcgtc atcggcgcca
     1141 cggtcttgtt ggtcgggagc gacggcgaca ccggcaacac cggcaacggg ggcagcggag
     1201 gcagcgacgg caacggcggg atggccggac gcggcacggt ggtcgtcgtc gtcgtgctcg
     1261 aaccgagcga cggactggtc accgcgacga tgcgcggctc ggtcaccggt gatccccctc
     1321 gcccgcgcgg cgtcgtcgtt ggcgccccgg gcgccgtcgt cggactcgtg gacgtcgtgg
     1381 tggacgatgt cgtccgccgc gcgatcgcct gcacgaagat ctgttgcacg ggcggctcgt
     1441 cgaacgtcgc gaacggctgg ccgttcgccc gccgggaggt gccggtgccg ccgtaacgat
     1501 cgacccggtc gttctgcggg ctgacgtaga tgtcgccgag cgcgaacagc acgagcaaga
     1561 cggagagtgc acacacgctc gcggtcaagg acagcagacg cgaccgtccc cggcggggcg
     1621 ccggtgtcgt gtgctccggt ttgtctccaa acactgcgtt gtgctctcag ccgaggggtc
     1681 tgtgcccgcg ttcacatcga cgggaggccg ctgttgtaaa ggccgcgccg ctcttcaccc
     1741 cacctggctg gacacgcgat ccggacgcta cgcgtcgttg gccgcgttgt cggtgcaatc
     1801 ttcctcttga gcagacgagc cgtccgcacg atcgcggcgg gattgatgcg gtgtgtgcgg
     1861 tttatagggc tgccggccgc gattgtcgcc gtcaccgacc ggatcggccg ccgccggagc
     1921 cgcagccagc gcgcccgcgg ccgctgccgc ttgctgcgcg tcgcgcgacc cgcgcgcgtc
     1981 cttcggttgg tgcggaccgt gcggccggtg cggacggtgc ttgccccgat ccttcttcgt
     2041 caccgcgggc gcttcggctt gcacgctcgc aacgaccacg gcggccggtt cggcgacgac
     2101 cggtgcgggc ggcgcgggtg cggtagccgc ggtcgtcggc gtcgtgacga ccgcggcgat
     2161 tgcgcgcacg tcgggaatcg gaaccgacgg gagctcgggc ggcacggtgg tcgtcgtcgg
     2221 cgcgtcgagg tcgacgccgg ggcccgaacg atcggtcacc acgccggcga tgcggccggc
     2281 actgcgcggc tcctgcggcg tcgcattgcg cacttcggag agcccgaaca tgacgagcag
     2341 cgtcgacaac gcggccaggc cacccacgat cgagacgatc ggcgacaacg ccgacgtacg
     2401 tctgctctcc actgccttgc gcacgtgtcg cagctccagc ccgcccggct ctcccgcaac
     2461 gtctcccacc aattcgtcgg cagaggaccg ccattttctg aagccgcggc tcaggaatcg
     2521 cacgagacga tgcgatgcac ccggacccgc gcgttgtcga acgtgagcaa tccgtacgtg
     2581 tggttcgtag cccggcgacg ctccgtgggc gaacccgggt tgaacaaccg ctgtccgtcg
     2641 acaccgatgc tgtcgtccgg ctggtggctg tggccgtaga cgacgaccgc ggcatccggg
     2701 aaacggcggt gcacccgcgc cgcgcggccc ttcgtcgcgc cggtctcgtg caccatcgcc
     2761 acccgcacac cgtcgaggtc gagcgtaacc gtctccggca gcacaccgac gagcgtgttg
     2821 tcgttgttgc cgagca
//
LOCUS       DASUGA010000303         2366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1916771, whole genome shotgun sequence.
ACCESSION   DASUGA010000303 DASUGA010000000
VERSION     DASUGA010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1916771"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFX21_05975"
     CDS             <1..345
                     /locus_tag="VFX21_05975"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX5095540.1"
                     /translation="SATAQFLGFPAPVAGVVEGGIVRTPWSNFPAPAGMPDGPVTVVL
                     RPDAVSLGGPIAATVRRVQFAGARVEITAQTESGESITLSAPARDAPAMNGRITVSID
                     ADAVLLYPHAAD"
     gene            350..973
                     /locus_tag="VFX21_05980"
     CDS             350..973
                     /locus_tag="VFX21_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEX5095541.1"
                     /translation="MPTKLEAAAAELAKRDRVLKRALRDHGVPELKRHRPRRPHFAEL
                     CRMICYQQLAGRAAAAIHGRFEALFDGPPTPEAVLMLSVEQMRGVGLSGAKTASIRDL
                     AEKVVAGLVELDRVAKLPDDEVVRELTLVRGIGEWTAHMFLMFQMTRLDVWPVLDLGV
                     RAGFARLYDVDPVPTPKQLVALGEPFRPYRSLVAVWCWDTVDTKTPG"
     gene            1013..1591
                     /locus_tag="VFX21_05985"
     CDS             1013..1591
                     /locus_tag="VFX21_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095542.1"
                     /translation="MADDSVIARRSRQRNRRALLTSLGLIGFAAVCITFIVNGFGAEP
                     GGATQVRQGKVYGALVTHPTTTTLAPTTTTTSTTTTTTVPPSTTTSSSTTTTTATTVP
                     PTTIATTTTTVPRPTVSAIVSNSPKFCTVTVRLSTGYQATYPLDQYVSNVGDTYSFNA
                     TIAGWRVDVTATVVNRNNTPACAATLANLQRV"
     gene            complement(1779..>2366)
                     /locus_tag="VFX21_05990"
     CDS             complement(1779..>2366)
                     /locus_tag="VFX21_05990"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5095543.1"
                     /translation="VDAAMVDGSAQLMSIFFGIDSIGGWGPRGTNLLDGGAHFYNVYE
                     TADGGYVSLAAYEPKFYANFLALVGSLGFDDLTVETQMDMAQWPAWKERLAVLFRTKT
                     RDEWVAFFAGHEICFAPILPMSEARAHPHNLDRHTFVDVDGAAHPAPAPRFSRSTTAV
                     QGAPVKPGADTETAFVEWGFDAAAVQKLTASGAIA"
BASE COUNT          379 a          810 c          805 g          372 t
ORIGIN      
        1 tccgcgaccg cgcagtttct cggtttcccg gctccggttg cgggcgtcgt cgagggcggc
       61 atcgtgcgca caccctggtc gaactttccc gcaccggccg gtatgccgga tggaccggtc
      121 acggtggtct tgcggcccga cgcggtttcg ttggggggac cgatcgccgc gaccgtgcgg
      181 cgcgtgcagt tcgccggcgc acgcgtcgag atcaccgcgc aaacggagtc cggggagtcg
      241 atcacgcttt cggcgccggc gcgcgatgcg ccggccatga acggacgaat cacggtgtcg
      301 atcgatgccg acgccgtgct gctctacccg catgcggcag actgacccga tgccgacgaa
      361 gctcgaagcg gcggccgccg aactcgcgaa acgcgaccgc gtactgaagc gtgcgctgag
      421 agatcacggc gtgcccgaac tgaagcggca tcgaccgcgt cgcccgcact tcgccgagct
      481 gtgccgcatg atctgttacc agcagctcgc gggccgggcg gcggccgcga tccacgggcg
      541 cttcgaagcg ttgttcgacg gacctccgac tcccgaagca gtgttgatgc tgtcggtcga
      601 acagatgcga ggtgtcgggc tgtcgggcgc gaagaccgca tcgattcgcg acctggccga
      661 gaaggtggtt gccggcctcg tcgaactcga tcgggtggcg aagctgcccg acgacgaggt
      721 ggtccgtgaa ctcacgctcg tgcgcggcat cggggagtgg accgcgcaca tgttcctcat
      781 gttccagatg acccgcctcg acgtgtggcc ggtgctcgat ctcggtgtcc gggccgggtt
      841 cgcccgcctg tacgacgtcg atcccgtgcc cacgcccaag cagctggtgg cgctgggtga
      901 acccttccgg ccctaccgct cgctcgtcgc ggtctggtgt tgggacaccg ttgataccaa
      961 aacccctggt tagggcgttg caactccggc gattcgcgcc gatcgtaaag gggtggcaga
     1021 cgactccgtg attgcgcgcc gcagccggca acgcaaccgg cgagcgctgc tgacgtcgct
     1081 cggcctcatc ggcttcgcgg cggtctgcat cacgttcatc gtcaacggtt tcggggccga
     1141 acccggcggc gccacccagg tgcgccaggg caaggtgtac ggcgcgctgg tcacgcaccc
     1201 gacgacgacg accctggcgc cgacgacgac cacaacgtcg acgaccacga ccacaaccgt
     1261 gccgccctcg accacgacca gttcgtcaac gacgacgaca acggcaacga ccgtgccgcc
     1321 gacgacgatc gccacgacta ccacgacggt gccgcgaccg acggtgagcg cgatcgtgtc
     1381 gaactcgccc aagttctgca ccgtcacggt gcggctgagc accggctacc aggcgaccta
     1441 cccgctcgat cagtacgtct ccaacgtcgg cgacacctac tcgttcaatg cgacgattgc
     1501 gggctggcgc gtcgacgtga ccgcaacggt ggtgaatcgc aacaacacgc cggcgtgcgc
     1561 cgcgacgctg gcaaacctgc agcgggtcta gcccggcgat gccggctgcg ttgcggccgc
     1621 gttgagatcg gtggcttggc gcgcgagttc ctcggcgcgc aaccacgcgg tttccggctg
     1681 gttgcgaagg gcggcagctt ccccggtgag gaacccacga cctcgccccg cccgacgaca
     1741 tcgagttcac cgagctcctg agcgccgacg ggcagcaact acgcgatcgc gccgctcgcc
     1801 gtgagtttct gcacggcggc ggcgtcgaag ccccattcga cgaaggcagt ttcggtgtcg
     1861 gccccgggct tgaccggcgc gccctgcacc gcggtcgtgg accgcgaaaa gcgcggtgcc
     1921 ggagccgggt gggcggcgcc gtcgacgtcg acgaaggtgt ggcggtcgag gttgtggggg
     1981 tgggcgcgcg cttccgacat cggcaggatc ggcgcgaagc agatctcgtg ccccgcgaag
     2041 aatgcgaccc actcgtcgcg cgtcttggtg cggaacagca cagcgaggcg ttccttccac
     2101 gccggccact gcgccatgtc catctgcgtc tcgaccgtga ggtcgtcgaa tccgagcgac
     2161 cccaccaacg cgaggaagtt cgcgtagaac ttcggttcgt acgcagcgag cgacacgtag
     2221 ccgccgtcgg ccgtctcgta gacgttgtag aagtgggcac cgccgtcgag caggttcgtg
     2281 ccgcgcgggc cccagccgcc gatcgagtcg atgccgaaga agatgctcat caactgcgcg
     2341 ctgccgtcga ccatcgcggc gtcgac
//
LOCUS       DASUGA010000304         2060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1717987, whole genome shotgun sequence.
ACCESSION   DASUGA010000304 DASUGA010000000
VERSION     DASUGA010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1717987"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1736)
                     /locus_tag="VFX21_05995"
     CDS             complement(<1..1736)
                     /locus_tag="VFX21_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5095544.1"
                     /translation="MPARKALAGAVGLALISGTALAQNPRPDPDEVVVTGKGAPLESA
                     STKFTAPLIDTPKSVTVIPQSLIAETGATTLVDALRTVPGITFNAGEGGQPAGDNLKI
                     RGFDAGADVFIDGVRDAGSQTRDVFALEQIEVVKGPGSAYSGRGSSGGTVNLVTKKPR
                     SENFIVTNLGAGTDSYGRAALDANYRFGDSGAFRLNVLRQDADVPGRDSVSQSHWGIA
                     PSLAFGIGEMTRVNLDVYRYETDDIPDYSIPYGRNAANTAPAGEPVDVDRENFYGLLD
                     RDFQRTSADVRTVQVTHTLGNGLTLTNTTRYGTTANDYIVTNPDDGRGNLVNGFLFRS
                     AKSRNSETETRANLTDFSGEARIGGLEHSYAFGVEASEEHMLNRPYVVESLFSGNAVT
                     AFPSSCSVPGVVGAPSSYNCTTLDDPNPHDPWTGTITQSATPTTVATDTRSVYGFDTL
                     GFNDRWSLNVGVRYDDYDTAQKGVSRGTPQDLENRSDFWNYQAGVVFKPKPNASVYLS
                     TGTSSTPPGNTLGDGTENLAANNQDLAPERDRTYELGTKWALFDDKLSLTSAVFNTET
                     ANARAAVVGGLQ"
BASE COUNT          300 a          656 c          744 g          360 t
ORIGIN      
        1 tgctgcaagc cgccgaccac ggcagcgcgg gcgtttgccg tctcggtgtt aaacaccgcg
       61 gaggtcagag acagcttgtc gtcgaacagc gcccacttcg tgccgagctc gtaggttcgg
      121 tcgcgctccg gcgccaggtc ctggttgttg gccgcgaggt tctcggtgcc gtcgccgagc
      181 gtgttccccg gcggggtcga cgaggtgccg gtcgagagat agacgctcgc gttcggcttg
      241 ggcttgaaca ccacgccggc ctgatagttc cagaagtccg accggttctc gagatcctgc
      301 ggcgtgccgc ggctcacgcc cttctgcgcc gtgtcgtagt cgtcgtagcg cacgccgacg
      361 ttgagcgacc agcgatcgtt gaagccgagc gtgtcgaatc cgtagaccga gcgcgtgtcg
      421 gtggccaccg tggtcggcgt ggccgattgc gtgatcgtgc cggtccaggg gtcgtgcgga
      481 ttcgggtcgt cgagcgtggt gcagttgtag ctcgacggcg cacccacgac gcccggcacg
      541 ctgcaagagc tcggaaaggc ggtcacggcg ttgcccgaga acagcgactc gaccacgtac
      601 ggccggttca gcatgtgctc ctcgctcgcc tcgacgccga acgcgtagga gtgctcgagt
      661 ccgccgatgc gggcctcgcc gctgaagtcc gtgagattcg cgcgcgtctc cgtctcggag
      721 ttgcggctct tggcgctgcg gaacaggaag ccgttgacga ggttgccgcg gccgtcgtcg
      781 gggttggtca cgatgtagtc gttggccgtg gtgccgtagc gcgtggtgtt ggtgagcgtg
      841 aggccgttgc cgagggtatg cgtgacctgc accgtgcgca cgtcggcgct ggttctttgg
      901 aagtcgcggt cgagcaaccc gtagaaattc tcgcggtcga cgtccacggg ctcgcccgcg
      961 ggcgccgtgt tggccgcgtt gcggccgtac gggatgctgt agtccgggat gtcgtcggtc
     1021 tcgtagcgat acacgtcgag gttcacgcgc gtcatctcgc cgatgccgaa tgcgagcgac
     1081 ggcgcgatgc cccaatgcga ttggctcacc gagtcgcggc ccggcacgtc cgcgtcttgg
     1141 cgcagcacgt tcaagcggaa cgcgccgctg tcgccgaacc ggtagttggc gtcgagggcg
     1201 gctcgtccgt agctgtcggt gccggccccg aggttggtga cgatgaagtt ctcggagcgc
     1261 ggcttcttgg tcacgaggtt caccgtgccg ccgctcgagc cgcggcccga gtacgccgac
     1321 cccgggccct tcactacttc gatctgctcg agggcgaaga cgtcgcgcgt ctgcgagccc
     1381 gcgtcgcgca ctccgtcgat gaacacgtcg gcgccggcgt cgaagccgcg aatctttagg
     1441 ttgtcgccgg ccggctgacc gccctcgccc gcgttgaacg tgatgccggg caccgtgcgc
     1501 agcgcgtcga ccaacgtggt ggcgcccgtc tccgcgatca gggactgcgg gatcaccgtc
     1561 accgacttcg gcgtgtcgat gagcggcgcg gtgaacttgg tggaggccga ttccagcggc
     1621 gcgcctttac cggtgacgac cacctcgtcc ggatcgggcc gcgggttttg cgccaaggcg
     1681 gtgcccgaga tcaacgcgag gccgacggcg ccggcgagcg ccttgcgagc gggcaacgcc
     1741 gatttcgatt gcgagcgagg catgtgaatc ctcccctgag gaaccaaccg cgcaaatgat
     1801 aatcgctcgc attggcgaac gcaatgagaa cgattcgcgt taatgtgtga acgcatcaca
     1861 atgcgtcgcg gtcagggaac gagcaggtgc gggccccgac gggccgacgg acctcggcgc
     1921 gtgccggagc acgccgaaaa aacggcggga gcgaaggaaa cgcgcgggcg ccgggcgcgc
     1981 ccgcggcggg ggttcagcgg gtggcgtcgc gagcgcggtc ggacatgccg tcctcccagc
     2041 tgctccagcc cgacgtcatg
//
LOCUS       DASUGA010000305         2173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_742721, whole genome shotgun sequence.
ACCESSION   DASUGA010000305 DASUGA010000000
VERSION     DASUGA010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742721"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFX21_06000"
     CDS             <1..274
                     /locus_tag="VFX21_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095545.1"
                     /translation="DTVTPSSIGPGPSQLTVAGARFLPGVTVAFAGGGITVDSVTRVD
                     ASTLLVDVTVANGVAPGGHTLGVTNPGTGPGAGAGGSKFCECISVS"
     gene            complement(436..509)
                     /locus_tag="VFX21_06005"
     tRNA            complement(436..509)
                     /locus_tag="VFX21_06005"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(473..475),aa:Val,seq:cac)
     gene            complement(525..1718)
                     /locus_tag="VFX21_06010"
     CDS             complement(525..1718)
                     /locus_tag="VFX21_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX5095546.1"
                     /translation="MTNLLDAARSVFDDVVALRRQIHSAPELGLELPRTQSAILTSLD
                     GLGLDVTTGTSVSSVVADLRGGAGDGPTILLRADMDALPMPEDTGLEFASTVDGTMHA
                     CGHDAHVAMLMGAARVLAAQRDSLRGTIRFAFQPGEEGFAGAKFMIDEGLLDDPNVDG
                     AFALHVAPNLPSGSLWTKGGPLMASADVISITVTGRGGHASTPHLANDPMPVAAEVIM
                     AIQTFVTRRIDAFDPAVITITKVRAGTTNNVIPEAVQMLGTVRTVSRSGRKRALEGLE
                     RVVAGIAAAHDMDATVSIDPGYPPTVNDAGFAAFVLEVASELLGADQTGEMPTPVMGA
                     EDFSYVLEERPGALAFLGVCPPGTNPARAASCHSNRMQLDENAMATGVALHCAVATRF
                     LARDR"
     gene            complement(1736..>2173)
                     /locus_tag="VFX21_06015"
     CDS             complement(1736..>2173)
                     /locus_tag="VFX21_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095547.1"
                     /translation="GIDNSRFANDLEGLQAKHAAELDDMERTGDRCPDLPQSVRDEVA
                     QYRAGFDELSNTLPNVARLTTEAAALARKLATQLDQAMQQRTATTLVDPATATLVEAA
                     RTLLLAQMSVTRATALAGSATASPPTFGLPPQVQAELKAFDKK"
BASE COUNT          330 a          729 c          780 g          334 t
ORIGIN      
        1 cgacacagtg acgccgtcgt cgatcggtcc cgggcccagt cagctgaccg tggcgggagc
       61 ccgattcctt ccgggcgtca ccgtcgcatt cgccggcggt ggcatcaccg tcgacagcgt
      121 cactcgcgtc gatgcatcga cgctgctcgt cgacgtcacc gtcgccaacg gcgtcgcacc
      181 gggcgggcac acgttgggcg tcaccaatcc cggcacgggt ccgggcgcag gtgccggcgg
      241 ctcgaagttc tgcgaatgca tctcggtgtc atgacgaacc gcgtttgatg agtccgacca
      301 cctcacgcga acaggtcgac gcgccacctc caacagggcg gtgccggagc ttgcggaggc
      361 actcgcggag tcctgaggcc ggccaacaat ccgggccgcc tcacgcgagc aggtcgaagg
      421 cgacaaagag ttgcgtgggc ggtagaggac tcgaacctcc gacctcatgc gtgtgaagca
      481 tgcgctctaa ccaactgagc taaccgcccc ggagggagga caggttagcg gtcccgcgcc
      541 aagaaccggg tggcgacggc gcagtggagg gccacgcccg tcgccatcgc gttctcgtcg
      601 agctgcatgc ggttcgagtg gcacgacgcg gcgcgcgcgg gattggtgcc gggcgggcag
      661 acgcccagga aggccagcgc gcccggccgt tcctcgagca cgtaggagaa gtcctcggcg
      721 cccatcaccg gtgtcggcat ctcccccgtc tggtcggcgc ccaacagctc ggacgcgacc
      781 tcgagcacga acgcggcgaa gccggcgtca ttgaccgtcg gcggatagcc gggatcgatc
      841 gagaccgtcg cgtccatgtc gtgcgcggcc gcgatgccgg cgacgacgcg ttcgagccct
      901 tcgagcgcgc gcttgcggcc gctgcgggac acggtgcgca cggtgcccag catctgcacc
      961 gcctcgggga tgacgttgtt cgtcgtgccc gcccgcacct tcgtgatcgt gatgacggcc
     1021 ggatcgaacg cgtcgatgcg acgcgtcacg aaggtctgga tcgccatgat cacctcggcc
     1081 gcgaccggca tcgggtcgtt ggcgagatgc ggcgtggacg cgtgaccacc gcgaccggtc
     1141 accgtgatcg agatgacgtc ggccgacgcc atcagcggtc cgcccttcgt ccacagcgat
     1201 ccggagggga ggttcggcgc gacgtgcaag gcgaacgcgc cgtcgacgtt ggggtcgtcg
     1261 agcagtcctt cgtcgatcat gaacttggcg ccggcgaagc cttcttcgcc gggttggaac
     1321 gcgaagcgga tcgtgccccg caacgagtcg cgctgtgcgg cgagcacccg ggctgcgccc
     1381 atgagcatcg cgacgtgcgc gtcatggccg cacgcgtgca tcgtgccgtc gacggtgctc
     1441 gcgaactcga gcccggtgtc ttcgggcatc gggagggcgt ccatgtcggc gcggagcaag
     1501 atggtcggcc cgtcgcccgc gccgccgcgc aggtcggcca cgaccgacga cacactcgtc
     1561 ccggtcgtca cgtcgagccc gagcccgtcg agcgaggtca ggatggcgga ctgcgtgcgg
     1621 ggcagctcga gccccagctc cggtgcgctg tggatctggc ggcgcaaggc gacgacgtcg
     1681 tcgaagacgg accgggcggc atccaacaag ttggtcatta cggcggagat tagggctact
     1741 tcttgtcgaa cgctttcagc tccgcttgca cctgtggcgg caggccgaac gtcggaggcg
     1801 acgcggtcgc cgagcccgcg agcgcagtgg cacgggtgac gctcatctgc gccaacaaga
     1861 gtgtccgcgc ggcctcgacg agcgtggccg tcgcgggatc gacgagcgtc gtcgcggtgc
     1921 gctgctgcat cgcctggtcg agctgggtgg cgagcttgcg cgccagcgcc gccgcctcgg
     1981 tcgtcagacg ggcgacattg ggcaaggtat tggacaactc gtcgaagccg gcccggtact
     2041 gcgcgacctc gtcgcgtacc gactgtggca ggtcggggca ccggtcgccg gtgcgctcca
     2101 tgtcgtccag ctcggccgcg tgcttggcct ggaggccctc gaggtcgttc gcgaaccggg
     2161 agttgtcgat gcc
//
LOCUS       DASUGA010000306         4105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1852645, whole genome shotgun sequence.
ACCESSION   DASUGA010000306 DASUGA010000000
VERSION     DASUGA010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1852645"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2272)
                     /locus_tag="VFX21_06020"
     CDS             complement(<1..2272)
                     /locus_tag="VFX21_06020"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5095548.1"
                     /translation="MFRLTFANIRMRKVRFLLTSVAVVLGVAFMAGTLVLTQTIKKSY
                     DDLAGAVYKDTDAVVRSADSVTDEANNELRAGVDGSFLDQVRGVPGVDVAEPSQLGIA
                     MVVGHDGDLLDASENRAVPIALAWQSDERLNPMELVSGHAPAANEIVIDKNSAEKGEF
                     AVGETIHVLSKAGAGDYVLSGIATYGGKDDAAGAQVVAFAPETAATVLGDPGRYDSID
                     IVAKPGVSQSQLAADVRATLHGDSVEVLTGQQAADDARELAGGKMQFLNTFLMAFAIV
                     GLLVGSFVIYNTFSITVAQRMRENALLRAIGAKRKQVMRSLVLESLFTGVFASAVGVA
                     AGIATAKGLGQVFEHFDVRLPDDGTVVTSSTIVISMITGTVVTVLAAYLPARRAGKVR
                     PIAALRDVSIDRSGTSKTRTVFGALLTAGGAALLGMGLSGGGIGPVGMGAMAIFVGVA
                     VLGPVLARPFARLIGSPLPRLRGMAGTIARENATRNPRRTSATASALMIGVGLVAFIT
                     VFAASTKASINKSVDTSVKTDLIVETAWGMGGLSPDAAAKLDALPETGIVSPVNYVST
                     EINGSAADLTGFDPQYVDVGLDLQATQGDLSKLGAHDLAISDNVAKDKGWKVGTQVPV
                     KFADTGVQTFTVTTIYDELGPTDGYAISLDALNANVADHVDNYLMVTQADGYSLAETK
                     AAAKGVLDEYPNAKVMTRDEFKGTIAQQIDQMLNLIYVLLFMALAIALFGIANTLALS
                     VFERTREIGLLRAVGMT"
     gene            complement(2274..3050)
                     /locus_tag="VFX21_06025"
     CDS             complement(2274..3050)
                     /locus_tag="VFX21_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009066406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5095549.1"
                     /translation="MSLTLSRPAQQGTVNGNAAVAARAVDAVKVYGKGDTEVRALDGV
                     TVDFATGRFTAIMGPSGSGKSTLMHTLAGLDDLTSGAVYIGDTDISRLRDKKLTKLRR
                     DRIGFIFQAFNLIPTLTAVENITLPMRLAGRKPDQAWVDNVVDTVGLRARLGHRPSEL
                     SGGQQQRVAVARALASRPQIIFADEPTGNLDSRSGAEVLSFMRQAVREFGQTIVMVTH
                     DPVAASYADRAVFLADGRIVDDLDNPTSDSVFDRMRSLGG"
     gene            3265..>4105
                     /locus_tag="VFX21_06030"
     CDS             3265..>4105
                     /locus_tag="VFX21_06030"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain-containing protein"
                     /protein_id="HEX5095550.1"
                     /translation="MERVPLSMRMSALARRIPMPLLDALIATAFLALSLAVLAGTEEG
                     DVDGLKGPTALAYLLTIAMVLPYYVRRRWPFVVYLTSLAFFCLLVVGDWEVDVPALLL
                     LIGTYTVGAYCASRDRLVAMLALAFTFLVVIISDIPGADGAGILVNVGFFGAAYFFGA
                     TVRNRRLYTEQLEERARALEHERDEQARRAVADERLRIAQELHDVVAHSMGVIAVQAG
                     VGAHVIDSDPVEAKKSLESISRVSRSTLTEIRRMLGVLREDEGAEYAPAPGLADLERL
                     VRDV"
BASE COUNT          644 a         1396 c         1384 g          681 t
ORIGIN      
        1 gggtcatacc gaccgctcgc agcaacccga tctcccgggt ccgttcgaac accgacagcg
       61 ccagcgtgtt ggcgatgccg aacagtgcga tcgccagcgc catgaacaac aacacgtaga
      121 tcaggttcag catctggtcg atctgctgtg cgatcgtccc cttgaactcg tcacgcgtca
      181 tcaccttggc gttggggtac tcgtcgagca cacccttggc ggccgccttg gtctcggcca
      241 gcgagtagcc gtcggcctga gtgaccatca ggtagttgtc gacgtggtcg gcgacgttcg
      301 cgttgagcgc gtccagcgag atggcgtagc cgtccgtcgg gccgagctcg tcgtagatcg
      361 tcgtgaccgt gaacgtctgc acaccggtgt cggcgaactt caccggcacc tgcgtgccga
      421 ccttccatcc cttgtccttg gcgacgttgt cggagatggc caagtcgtgc gcacccagct
      481 tcgacaggtc gccctgcgtc gcctggaggt cgagcccgac gtcgacgtac tgcgggtcga
      541 agccggtgag gtcggcggcc gaaccgttga tctcggtcga cacgtagttc accggtgaca
      601 cgatgccggt ctccggcagc gcgtccagct tcgcggcggc atcggggctc aggccgccca
      661 tgccccacgc cgtctcgacg atgaggtcgg tcttcaccga cgtgtcgacc gacttgttga
      721 tcgacgcctt ggtcgacgcc gcgaacaccg tgatgaacgc caccagaccg acaccgatca
      781 tcagtgccga cgccgtcgcc gacgtgcgcc gcgggttgcg ggtcgcgttc tcccgggcga
      841 tcgtgccggc catgccgcgc agccgcggca acggcgaacc gatcaaccgt gcgaacgggc
      901 gggccagcac cgggccgagc accgcgacgc cgacgaagat cgccatcgca cccatgccga
      961 ccggaccgat accgccaccg gacaaaccca tccccagcag cgcggcgccg ccggccgtca
     1021 acagcgcacc gaagaccgtg cgggtcttcg acgtgccgct gcggtcgatc gacacgtcac
     1081 gcagcgccgc gatggggcgg accttcccgg cccgccgagc gggcaggtag gcagccaaca
     1141 ccgtcacgac cgtgcccgtg atcatcgaga tgacgatcgt gctcgacgtg acgacggtgc
     1201 cgtcgtcggg cagccgcacg tcgaagtgct cgaacacctg gccgaggccc ttcgccgtcg
     1261 cgatgcccgc ggccacaccg acggccgagg cgaacacgcc ggtgaacaac gactcgagca
     1321 cgagcgaacg catcacttgc ttgcgcttcg caccgatcgc ccgcagcaac gcgttctcac
     1381 gcatccgctg cgcaacggtg atcgagaacg tgttgtagat gacgaacgaa ccgacgagca
     1441 ggccgacgat cgcgaacgcc atcaggaacg tgttcaggaa ctgcatcttg ccgcccgcga
     1501 gctcgcgcgc gtcgtccgca gcttgctggc cggtgagcac ttcgacgctg tcgccgtgca
     1561 acgtggcgcg cacgtccgcg gccagctgcg actgcgacac accgggcttg gccacgatgt
     1621 cgatcgagtc gtagcgacca gggtcaccga gcacggtcgc ggccgtctcg ggcgcgaacg
     1681 ccaccacttg cgcaccggcc gcgtcgtcct tgccgccgta ggtcgcgata ccggagagca
     1741 cgtagtcgcc ggcgccggcc ttcgacaaca catggatcgt ctcgccgacc gcgaactcgc
     1801 ccttctcggc cgagttcttg tcgatgacga tctcgttcgc ggccggtgca tgtccggaca
     1861 cgagctccat cgggttgagg cgctcgtccg actgccacgc gagcgcgatc ggcacggcgc
     1921 ggttctcgct cgcgtcgagc aagtcgccgt cgtggccgac caccatcgcg atgccgagct
     1981 gcgacggttc cgcaacgtcg acacccggaa cgccgcgcac ctggtcgagg aaggatccgt
     2041 cgacgccggc gcgcagctcg ttgttggctt cgtcggtgac gctgtcggcc gaacgcacga
     2101 ccgcgtcggt gtccttgtac accgcaccgg cgaggtcgtc gtacgacttc ttgatggtct
     2161 gggtgagcac gagcgtgccc gccatgaacg cgacaccgag cacgacggcg acgctggtga
     2221 gcaggaaccg gaccttgcgc atccggatgt tggcgaacgt caggcggaac atctcagcct
     2281 cccaggctgc gcatgcggtc gaagaccgag tcggacgtcg ggttgtcgag gtcgtcgacg
     2341 atgcgaccgt cggccaggaa gaccgcgcgg tccgcgtacg aagcggcgac cgggtcgtgc
     2401 gtcaccatca cgatcgtctg gccgaactcc cgtacggcct gacgcatgaa ggagaggact
     2461 tcggcacccg agcgggagtc gaggttcccc gtcggttcgt cggcgaagat gatctgcggg
     2521 cggctggcga gcgcacgggc gacggcaacg cgctgttgct gaccacccga gagctccgac
     2581 ggacggtgac cgaggcgtgc ccgcagaccg acggtgtcga cgacgttgtc gacccaggcc
     2641 tggtcgggct tgcggcccgc gagacgcatc gggagggtga tgttctcgac ggcggtcagc
     2701 gtcggaatga ggttgaacgc ctggaagatg aaaccgatgc ggtcacgccg aagcttcgtg
     2761 agcttcttgt cgcgcaaccg cgagatgtcg gtgtcgccga tgtagacggc accgctcgtg
     2821 agatcgtcga gacccgcgag cgtgtgcatg agcgtcgact tgcccgaacc cgacgggccc
     2881 atgatcgcgg tgaagcgacc ggtcgcgaag tcgacggtga cgccgtcgag cgcgcgcacc
     2941 tcggtgtcgc ctttgccgta gaccttgacc gcgtcgacgg cccgggcggc aacggcggcg
     3001 ttgccgttga ccgttccctg ctgtgcgggg cgagagagtg tgagtgacat ggactggctc
     3061 ctcggttgag agcccgtccg tatcgggctc tggagccacc atgcgccgat cggccggcgg
     3121 gatcgtcggc cgcgagtcgc tgggcgatca gcctccagtc gcctcgtgcc ggcgccgagc
     3181 ggccgccgcg tacatctcgg gatgtacgcc ggagtcgccc tcgggatgga tgccgccggg
     3241 ctgacctgtc ggtacgttga ccaggtggaa cgtgttcccc tgtccatgcg gatgtcggcc
     3301 ctggcccggc gcatcccgat gccgctgctc gacgccctca tcgcgaccgc gttcctggcg
     3361 ctgtcgctcg ccgtgctcgc cggtaccgag gagggcgacg tcgacggtct gaagggcccg
     3421 actgctctcg cgtacttgct gacgatcgcg atggtgctcc cgtactacgt gcggcggcgc
     3481 tggccgttcg tcgtgtatct gacgtccctc gcgttcttct gcctgctcgt cgtcggcgac
     3541 tgggaggtcg atgttccggc gctgctcttg ttgatcggga cgtacaccgt cggcgcgtac
     3601 tgcgcctcga gagatcgtct cgtggcaatg ctcgcgctcg cgttcacctt cttggttgtc
     3661 atcatctccg acataccggg tgccgacggt gccggcatcc tggtcaacgt cggcttcttc
     3721 ggcgcggcgt acttcttcgg cgcgaccgtg cgcaaccggc gtctctacac cgagcagctc
     3781 gaggaacgcg cgcgcgcgct cgaacacgaa cgcgacgagc aggcgcggcg cgccgtcgcc
     3841 gacgagcgcc tgcgcatcgc acaggaactg cacgacgtcg tcgcccattc gatgggtgtc
     3901 atcgcggtgc aagcgggcgt cggcgcgcac gtcatcgaca gcgaccccgt cgaggcgaag
     3961 aagtcgctcg agtcgatctc tcgcgtcagc cggtcgacgc tcaccgagat ccgccgcatg
     4021 ctcggtgtgc tgcgcgaaga cgaaggtgcg gagtacgcgc ccgcgcccgg gctggccgat
     4081 ctcgaacggc tggtgcgcga cgtgt
//
LOCUS       DASUGA010000307         2897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1816366, whole genome shotgun sequence.
ACCESSION   DASUGA010000307 DASUGA010000000
VERSION     DASUGA010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816366"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="VFX21_06035"
     CDS             <1..980
                     /locus_tag="VFX21_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095551.1"
                     /translation="FRSAMAELITLLSQTGVYVVVLNGSTVVPDDVAHCYRDGEETPA
                     LIMHRLNAALMELAAGEGISILDADRVIAELGGEHHVTGLLSYSDVANDALCAELVAI
                     LNESGYLDGRARRAGESRTATGSALRLEMVAHTAAVRAGRVRRWRVAEGASFARGDGL
                     VDIDVTEWSTLARRRTRPHMGPADERRDRLTFDAPRRPTVVHIVARERGVLARVTAPV
                     GTPVAIGDLLGVVGVSEGLGPWASAPPPGGPTLQTGAVRFRERADRVEIDESRAASTP
                     APSPRRKAAVSHSTLYTRCKRLVPKPLRRRARSLLRRFREKPRHPGAAA"
     gene            977..1876
                     /locus_tag="VFX21_06040"
     CDS             977..1876
                     /locus_tag="VFX21_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095552.1"
                     /translation="MSDANEAREVVQGDTFVRWSKPRSTVIRSLVNPEPRTGIFLKGA
                     CDLPALLLLGELLQGQVRGTVAVFADKGAIAYSRADILLQSLEEIPTEATQELFDHLP
                     VPPDYFRPTLFEPSFTPPHRLGEFPKTVVVLSLVPNVIRTAYKDRKHGYLLDPGAWWL
                     DEERFAKGEKERLDWIRKRFTTIGRLKIEEFVELFTKLILTVQERLDAHVLVFNVLTV
                     EPGLRVHNYRMRKAPDGLRRREFNIALAELSGPLGFHIVDVDRTLKRYGISRQQDFDH
                     FPVDAGLPIATEALRILRELEVL"
     gene            1873..>2897
                     /locus_tag="VFX21_06045"
     CDS             1873..>2897
                     /locus_tag="VFX21_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5095553.1"
                     /translation="MSVTHAPRGSERSGAAPGTLLHRRLEASARDRPDAIAVVDGDRS
                     FTYAQLAADASRVAHLLRECGIRRGDRVGLYLEKSYEGIAGVYGTLAAGAAYVPLDPR
                     GPAPRLGYIAADCGLTCLLSSRAHRARVRDLCANGALVEHLVLLDELPPVPASPLSSA
                     AGATEDDLAYILYTSGSTGVPKGVMLSHRNALAFVDWAVAVTGAGPEDRLSSHAPLHF
                     DLSVFDLYAASSVGAALVLVPHKVALFPIEQTRFIAANEITVWYSVPSTLSMMVEKGG
                     LARGDLPSLRTVIFAGEVFPTRYLHALMTLLPHVEFWNWYGPTETNVCTGYRVPEPPD
                     PAGPDIP"
BASE COUNT          433 a         1005 c          972 g          487 t
ORIGIN      
        1 ggttccggtc cgcgatggcg gagctcatca cgctgttgtc gcagaccggt gtctacgtcg
       61 tcgtcttgaa cggttccacg gtcgttcccg acgacgtcgc ccactgctac cgcgacggcg
      121 aggagacgcc cgcgctcatc atgcatcgct tgaacgccgc gctcatggag ctggccgccg
      181 gcgaaggcat ctcgatcctc gacgcggacc gcgtcatcgc ggagctgggc ggcgaacacc
      241 atgtcaccgg cctcctttca tacagcgacg ttgcgaacga cgcgctctgc gccgaactcg
      301 tcgcgatcct caacgagtcg ggatacctcg acggccgcgc acgacgcgcg ggtgagagcc
      361 ggacggctac gggctcggcg ttgcggctcg agatggtcgc gcacaccgcc gcggtccgcg
      421 ccggccgcgt gcgccggtgg cgcgtcgccg aaggcgcatc gttcgcgaga ggcgacgggc
      481 tcgtcgacat cgatgtgacc gagtggtcca ccctcgcccg gcggcggact cgtccgcata
      541 tgggaccggc ggacgagcgc cgggatcggc tgacgttcga cgcgccccgg cggcccaccg
      601 tcgttcacat cgtcgcgcgg gagcgagggg tgctcgcacg ggttacggcc ccggtgggaa
      661 cgcccgttgc catcggcgat cttctcggtg tggtcggggt cagcgagggc ctaggtccgt
      721 gggcgtccgc accgccgccg ggcggcccga cgctgcaaac gggtgcagtg cgctttcgag
      781 aacgcgccga ccgcgtcgag atcgacgagt caagggcggc gagcactccc gcgccttcgc
      841 cacgacgcaa agccgccgtg tcgcattcca cgttgtacac gcggtgcaag cggctcgtgc
      901 caaagccgct gcgacgccgc gctcggtcgt tgctacgccg atttcgagag aagccacgcc
      961 acccgggggc ggccgcgtga gtgatgcgaa cgaggcacga gaagtcgttc agggcgacac
     1021 gttcgttcgg tggagcaaac cgcgcagcac tgtcattcgt tcactcgtca atccggagcc
     1081 gcgaacgggg atcttcctca aaggcgcgtg cgatcttccc gcactgctct tgttgggtga
     1141 gctcttgcag ggccaggtgc gaggcaccgt tgcggtattc gcggacaagg gcgcgatcgc
     1201 gtactcgcgc gccgacatcc tcctccagtc gctggaggag atcccgaccg aagcgacgca
     1261 ggagctgttc gaccacttgc cggtgccgcc ggactatttc cggccgacgc tcttcgagcc
     1321 ttcgttcacg cctccgcacc ggctgggcga gttcccgaag accgtcgtcg tcctgtccct
     1381 cgtgcccaac gtcattcgca cggcgtacaa ggatcgcaag cacggctacc tgctcgatcc
     1441 cggcgcgtgg tggctcgacg aggaacgctt cgcgaagggc gaaaaggaac ggctcgattg
     1501 gatccggaag cgcttcacca cgatcgggcg gctcaagatc gaagagttcg tggagctgtt
     1561 cacgaagctc atccttaccg tgcaggagcg tctggacgca cacgtgctcg tgttcaacgt
     1621 cctgacggtc gagccaggat tgcgggtgca caactaccgc atgcgcaagg ctcccgacgg
     1681 gctgcgccgg cgcgagttca acattgcgct cgcggagctc tccggcccgc tcggcttcca
     1741 catcgtcgac gtcgaccgca cgttgaaacg ctacggcatc tctcggcagc aggacttcga
     1801 tcacttcccg gtcgacgccg gtctccccat cgccacggag gcactccgga tcctgcgcga
     1861 gctcgaagtg ctatgagcgt cacgcacgcg ccccgaggat cggagcgttc gggcgccgcg
     1921 cccggcacgc tcctccatcg ccgattggag gcgagcgcgc gcgatcgacc cgacgcaatc
     1981 gcggtcgtcg acggcgaccg ttccttcacc tatgcgcagc tcgcggcgga cgcgtcgcgc
     2041 gtcgcgcacc tgctgcgcga gtgcggcatc cgccgcggtg accgcgtcgg gctgtacctg
     2101 gagaagtcct acgaagggat cgccggtgtg tacggcacgc tcgcggcggg cgcggcctac
     2161 gtccctctgg acccacgcgg gccggcaccg cggctcggct acatcgccgc cgactgcggc
     2221 ctgacgtgcc tcctcagcag ccgggcgcac cgcgcgcgtg tccgcgacct gtgcgccaac
     2281 ggcgcgctcg tcgagcacct cgtgttgctc gacgagttgc cgcccgtccc cgcgtcaccg
     2341 ctctcgtcgg ccgcgggcgc gaccgaagac gatctcgcgt acatcctcta cacgtcggga
     2401 tctaccggtg tgccgaaggg cgtgatgctc agccaccgca atgcgctcgc gttcgtcgac
     2461 tgggcagttg ccgtgaccgg tgccgggccc gaggaccggc tgtcgagtca cgcgccgttg
     2521 cacttcgacc tgtcggtctt cgatctgtac gcggcctcgt cggtcggcgc cgcgctcgta
     2581 cttgtcccgc acaaggtcgc gttgttcccc atcgagcaga cgcgcttcat cgcggccaac
     2641 gagatcacgg tgtggtactc ggtgccgtcg acactcagca tgatggtcga gaagggtggt
     2701 ctcgcacgcg gtgacctgcc gtcgttgcgc accgtgatct tcgcggggga ggtgttcccg
     2761 acgcgttatc tgcacgcgtt gatgacgctg ctgccgcacg tcgagttctg gaactggtac
     2821 gggccgacgg agaccaacgt gtgcacgggg tatcgcgtgc ccgagccgcc ggatcccgcc
     2881 gggcccgaca tcccgat
//
LOCUS       DASUGA010000308         3792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1574520, whole genome shotgun sequence.
ACCESSION   DASUGA010000308 DASUGA010000000
VERSION     DASUGA010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574520"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..521)
                     /gene="folK"
                     /locus_tag="VFX21_06050"
     CDS             complement(42..521)
                     /gene="folK"
                     /locus_tag="VFX21_06050"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX5095554.1"
                     /translation="MTVAYLALGSNIGDRRAYLQLAVDTLAATPGVQLLAVSRVYETD
                     PVGGPVQDDYYNAVVALETDLDPWALLALAQRCEEAAHRVRRERWGPRTLDVDVLLYD
                     DLRLDDPALTVPHPRMWERDFVLVPLADVAPERVPAGRAGKGVRVAPVALVLSPPSP"
     gene            complement(518..868)
                     /gene="folB"
                     /locus_tag="VFX21_06055"
     CDS             complement(518..868)
                     /gene="folB"
                     /locus_tag="VFX21_06055"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:NF014233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX5095555.1"
                     /translation="MDRIVISGLREVGIHGVLPEERVRPQPFEIDVELYVDLAAAGES
                     DDLGDTVDYGAVSEAVARIVRDEHHQLLERLAQRIATQCRVDERVQRVVVEVRKLRPP
                     IPLPLAHVAVRIER"
     gene            903..2318
                     /gene="pyk"
                     /locus_tag="VFX21_06060"
     CDS             903..2318
                     /gene="pyk"
                     /locus_tag="VFX21_06060"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX5095556.1"
                     /translation="MRRTKIVATVGPATSSDDALRSILEAGADVVRLNAAHGTPDVHA
                     ERAQHVRNVARAIGRSVGVLVDLPGPKLRTGVIAGDDVELDPGQRFVLTADDAPGDDT
                     RVGTTVPELARWVRPRDDIYLADGEIVLCAIAVEGDDVVTEVVRGGLLRSRKGMHVPR
                     AEGHVDPFTDADEAALAMSVKIKADYVGLSFVRRPDDVDAVRARLPKRGIRPLLVAKI
                     ETAVALDHLAGIVASADAVMVARGDLGIQVPARRVPLIQKEIIRFCNMSGKPVITATQ
                     MLESMTRAPLPTRAEVNDVANAVIDGTDALMLSEETAVGAHPADAVRTMAEVADSAET
                     WPRSRVTPAGAGAAADDDRVAWAVAHAAVQAAEDLSVTAIVCMTQSGNTARRVAAFRP
                     SVPVVAASPQSDALGRLGLVWGVRPLLVPLAEAQNEHLTLALRSATDARLLQSGDLVT
                     VVSASPGPRAGSTDLVRVVRA"
     gene            complement(2328..2852)
                     /locus_tag="VFX21_06065"
     CDS             complement(2328..2852)
                     /locus_tag="VFX21_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095557.1"
                     /translation="MNTSGLRGRWAQGLQPRYFTWFIKDRLGAGERPGGFARNHRKVR
                     RQEELIWLRVNNFTHLISLLDSPHNLHAYEEAGLAYAHTPLGPSDELGIRLKTIYDTV
                     GGYLDDPNEKLYLHHEEFGERVIGVLGGYLLYTGVVETGPHAISIVEKLTGRSLGAAG
                     REIIATTLNERISR"
     gene            complement(2849..3658)
                     /gene="folP"
                     /locus_tag="VFX21_06070"
     CDS             complement(2849..3658)
                     /gene="folP"
                     /locus_tag="VFX21_06070"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX5095558.1"
                     /translation="MGVVNVTPDSFSDGGAFLSVDAAIAHGRQLARDGAAVLDVGGES
                     TRPGAAPVDADEELRRVVPVVRALAAEPGVYVSIDTTKARVAEAAIESGASIVNDVSG
                     GRADAAMHDVIASTGAGYVAMHMRGTPRTMQREATYHDVVADVGRELRDRVDAALAVG
                     VRADGLMADPGIGFAKTADQNVELLRALPELARYAGVPLLVGASRKSFLGALLGDLPV
                     GERDEATLATTVWCFTTGAAMVRVHDVESSVRAAQLMDVMERATATGMVAA"
BASE COUNT          602 a         1347 c         1288 g          555 t
ORIGIN      
        1 tcatggaaca cgagaacacc tcggccagtg ctccccgcgg ttcaggggga tggcggggag
       61 agcaccaagg ctaccggagc cacgcgtacc ccttttcccg cccgacctgc gggaacacgc
      121 tccggcgcga cgtcggccag cggcaccagg acgaaatcgc gttcccacat gcgcgggtgg
      181 gggacggtca gcgccgggtc gtcgaggcgg aggtcgtcgt acagcaacac gtcgacgtcg
      241 agcgtgcgcg gcccccagcg ctcgcgccgt acgcggtgcg cggcctcctc gcatcgttgc
      301 gcgagcgcga gcagtgccca cggatcgagg tcggtctcga gcgcgacgac ggcgttgtag
      361 tagtcgtcct gcacggggcc gccgacggga tccgtctcgt agacgcgcga gacggccagc
      421 agctgcacgc ccggtgtcgc ggcgagcgtg tcgacggcga gctggaggta cgcgcgccgg
      481 tcgccgatgt tcgagccgag cgcgaggtac gcgaccgtca acgctcgatc cgcaccgcga
      541 cgtgcgcaag cggcagcgga atcggcggcc gcaatttccg gacttcgacg acgacccgtt
      601 ggacgcgttc gtcgacgcgg cactgcgtcg cgatgcgttg cgccagccgc tcgaggagtt
      661 gatggtgctc gtcacggacg atccgggcga ccgcttctga gacggcgccg tagtcgacgg
      721 tgtcgccgag atcgtcgctc tcgcccgcgg cggcgaggtc gacgtacaac tccacgtcga
      781 tctcgaacgg ctgcggccgg acccgctcct cgggcagcac gccgtgaatg cccacctcgc
      841 gcaggcccga aatcacgatc cgatccacga acccgacgct accccggccg atacttgagt
      901 tgatgcggcg tacgaagatc gttgccaccg ttggtcccgc gacatccagt gacgatgcac
      961 tccggtcgat cttggaggcg ggtgccgatg tcgtccgcct gaacgcggcc cacggaacac
     1021 cggacgtcca cgcggaacgg gcgcaacacg tccgcaatgt cgcgcgcgcc attgggcgaa
     1081 gcgtcggcgt gctcgtcgac ctgcccggcc cgaagctgcg caccggtgtg atcgccggtg
     1141 acgacgtcga gctcgatccc ggacaacggt tcgtgctcac cgccgacgac gcgccgggcg
     1201 acgacacgcg tgtgggcacc acggtgcccg agctcgcgcg ttgggtccgc ccgcgcgacg
     1261 acatctacct ggccgacggc gagatcgtgc tgtgcgcgat cgcggtcgaa ggtgacgacg
     1321 tggtgaccga ggtcgtgcgc ggcgggctcc tgcggtcacg caagggcatg cacgtcccgc
     1381 gcgccgaggg tcacgtcgac ccgttcaccg acgccgacga agcggcgctc gcgatgtcgg
     1441 tgaagatcaa ggccgactac gtcgggctgt ccttcgtccg ccgccccgac gacgtcgacg
     1501 cggtgcgcgc ccggctcccc aaacgcggca tccgcccgtt gctcgtggcc aagatcgaga
     1561 ccgcggtcgc cctcgaccac ctcgccggca tcgtcgccag tgccgacgcg gtcatggtgg
     1621 cgcgtggtga cctcggtatc caggtgccgg cacgccgcgt gccgctcatc caaaaggaga
     1681 tcatccggtt ctgcaacatg tcggggaagc cggtgatcac cgcgacacag atgctcgagt
     1741 cgatgacgcg cgcgccactc ccgacgcgcg ccgaggtcaa cgacgtcgcc aacgcagtca
     1801 tcgacggcac tgacgcactg atgctgtcgg aggaaactgc ggtcggcgcg catccggcgg
     1861 acgcggtgcg caccatggct gaggttgcgg actccgcgga gacgtggccg cgcagccggg
     1921 tcacaccggc cggagccggc gcggccgccg acgacgaccg ggtggcgtgg gccgtcgcgc
     1981 acgccgcggt gcaggcggcc gaggacctct cggtcaccgc gatcgtgtgc atgacccaga
     2041 gcggcaatac ggcgcgccgc gttgccgcgt tccggccgtc ggtgccagtc gtcgcagcat
     2101 caccgcaaag cgacgcactg ggccggctcg ggctcgtgtg gggcgtacgc ccgttgctgg
     2161 tgccgctcgc cgaggcgcaa aacgagcacc tcacgctcgc gctgcgcagc gcgaccgacg
     2221 cgcgtctgtt gcagtccggc gacctggtga cggtggtttc cgcatcgccg ggcccgcggg
     2281 cgggcagcac cgacctcgtc cgcgtcgtac gcgcgtagcg caggttgcta gcgcgagatt
     2341 cgttcgttca gtgtcgtcgc gatgatctcg cggcccgcgg cgccgaggct gcggccggtc
     2401 agcttctcga cgatcgagat cgcgtgcggt ccggtctcga cgacaccggt gtagagcagg
     2461 tagccgccga gcacaccgat cacgcgctcg ccgaactcct cgtggtgcag gtagagcttc
     2521 tcgttcggat cgtcgagata tccgccgacg gtgtcgtaga tcgtcttcag ccgaatgccg
     2581 agctcgtcgc tgggcccgag cggcgtgtgc gcgtacgcga gccccgcctc ttcgtacgca
     2641 tgcaggttgt gcggcgagtc gagcaacgaa atcaggtgcg tgaagttgtt cacgcgcagc
     2701 cagatcagct cctcctggcg ccgcaccttg cggtggttgc gcgcgaaccc gcccggccgc
     2761 tcacccgcac cgagccggtc cttgatgaac cacgtgaagt aacgcggctg aagcccttgc
     2821 gcccaccgac cccgcaaccc actcgtgttc acgctgcaac catccccgtt gccgtggccc
     2881 gttccatcac atccatcaac tgtgccgccc gcaccgacga ctcgacgtcg tgcacgcgaa
     2941 ccatcgccgc accggtggtg aagcaccaca cggtcgtggc cagtgtcgct tcgtcacgtt
     3001 cgcccaccgg cagatcgccg agcaacgcac cgaggaacga cttacgcgac gcaccgacca
     3061 acagagggac acccgcgtac ctcgcaagct ccggcagcgc acggagcagc tccacgttct
     3121 gatctgccgt cttcgcgaag ccgatgccag ggtcggccat caatccgtcg gcccggacac
     3181 cgaccgcgag cgcggcgtcg acacggtccc gcagttcgcg cccgacgtcg gcgacgacat
     3241 cgtggtacgt cgcttcgcgc tgcatcgtgc gaggcgtacc ccgcatgtgc atcgcgacgt
     3301 agcccgcgcc cgtgctcgcg atgacgtcgt gcatggctgc gtcggcccgg ccacccgaca
     3361 cgtcgttcac gatcgacgcg ccggactcga tcgcggcctc ggcaacgcgc gccttcgtgg
     3421 tgtcgatgct gacgtagaca ccaggctccg ccgcgagcgc gcgaacgaca ggaacgacgc
     3481 gacgcagctc ttcgtcggcg tcgaccggcg cggcgcccgg acgcgtcgac tcgccgccga
     3541 cgtcgagcac cgcggcgccg tcacgcgcga gttgccggcc gtgcgcaatc gcggcgtcga
     3601 ccgagagaaa cgcgccgccg tcggagaagg aatcgggcgt gacgttcacg acgcccatca
     3661 ccgcccgtcg cggcagcgag gcgacggtga aggacgaggg cacgagccgc agaatatcga
     3721 accgacacct cgtacccgct cacgtttggc gggtaattca gaggaatttc tagagcgttc
     3781 ggctgcccat cc
//
LOCUS       DASUGA010000309         2362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_388740, whole genome shotgun sequence.
ACCESSION   DASUGA010000309 DASUGA010000000
VERSION     DASUGA010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388740"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /locus_tag="VFX21_06075"
     CDS             complement(<1..1345)
                     /locus_tag="VFX21_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX5095559.1"
                     /translation="MTSNLEELGPNAGLVDEMYRLYRDNPQAVSAGWREFFEDYVPVG
                     ETAPATPPAPAPAAAAPAPAPAPAAAPAPAPALSGPGAPVVLEGEQPAPLRGAAARIV
                     ANMEASLGVPTATSVRSVPAKLLEVNRQILNNHLQRTRGGKVSFTHLIGFAVLRALRI
                     VPNMNSSFGVVDGTPSVVRHEHVNLGLAIDLQKDDGSRTLLVPNIKSADTLDFAAFHA
                     QYEELIRKARTNKLAVDDFAGTTVSITNPGTIGTMHSVPRLMPGQGTIIGVGAITYPI
                     EYEGADPQTLADIGVSKVVTLTSTYDHRVIQGAESGEFLSCVHELLLGEHGFYDEMFA
                     SFGVPYEPARWGRDHKPLEGSHEALKKALAVNGLVNMYRVRGHLIANLDPLSLKEPRT
                     HEELDPVHWGLTIWDLDREFPTSVAGKDVMKLRDILGVLRDAYSRTIGVEYMHIQE"
     gene            complement(1641..>2362)
                     /locus_tag="VFX21_06080"
     CDS             complement(1641..>2362)
                     /locus_tag="VFX21_06080"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX5095560.1"
                     /translation="RAMRFMAACGIDLQAEFQLHEVDFFTSHDALLLGYEEALTRRDS
                     LTGDWYDCSAHLLWIGERTRQIDGAHVEFLSGVQNPIGLKLGPDATPEQVLALCRRLD
                     PGRDPGRLTLISRMGATRVAEALPPLLRAVKRAGHPVVWACDPMHGNTFSHESYKTRH
                     FDDVLREVEQFFDVCKAEGVWPGGVHIEMTGDDVTECLGGAEEIRGEHLAGRYETQCD
                     PRLNGRQSLDLAFRVSELVRR"
BASE COUNT          345 a          789 c          838 g          390 t
ORIGIN      
        1 gctcctggat gtgcatgtac tcgacaccga tcgtgcgcga gtacgcgtcg cgcaacacgc
       61 cgaggatgtc gcgcagcttc atgacgtcct tgcccgcgac gctcgtcggg aactcgcggt
      121 cgaggtccca gatcgtcaag ccccagtgca ccgggtcgag ctcttcgtgg gtgcgcggtt
      181 ccttgagact cagcggatcg aggttcgcga tgaggtgacc gcggacgcga tacatgttga
      241 cgaggccgtt caccgcgagc gccttcttca gcgcttcgtg cgagccctcg agtggcttgt
      301 ggtcgcggcc ccaacgggcg ggctcgtagg ggaccccgaa gctggcgaac atctcgtcgt
      361 agaacccgtg ttcacccagc aacagctcgt gcacacacga gaggaactcg ccgctctcgg
      421 cgccctggat cacacggtgg tcgtacgtgc tcgtgagcgt gacgaccttg ctgacgccga
      481 tgtcggcgag cgtctgcggg tccgcgcctt cgtactcgat cggatatgtg atcgcgccga
      541 cgccgatgat cgtgccctgg cccggcatga ggcgcggcac cgagtgcatc gtgccgatcg
      601 tgcccgggtt ggtgatcgac accgtcgtcc ccgcgaagtc gtcgaccgcg agcttgttgg
      661 tgcgcgcctt gcggatcagc tcttcgtact gcgcgtggaa cgcggcgaag tcgagcgtgt
      721 cggcgctctt gatgttcggg acgagcagcg tgcgcgaccc gtcgtccttc tgcaggtcga
      781 tcgcgagccc gaggttcacg tgctcgtgcc gcacgaccga cggtgtgccg tcgaccacgc
      841 cgaacgacga gttcatgttg gggacgatgc gcagggcccg cagcaccgcg aagccgatca
      901 ggtgcgtgaa cgagaccttg ccgccgcgcg tgcgctgcag atggttgttg aggatctggc
      961 ggttgacctc gagcagtttc gcgggcaccg accgcaccga tgtcgcggtc gggacgccga
     1021 ggctggcctc catgttggcg acgatgcggg cggccgcgcc gcgcaacggt gcgggctgct
     1081 cgccctcgag cacgacaggc gcgccgggac ccgagagcgc cggtgcgggt gcgggtgcgg
     1141 cggccggggc cggtgccggt gcgggtgcgg cagcggccgg tgcgggtgcg ggcggggttg
     1201 cgggcgcggt ctccccgacc ggcacgtagt cctcgaagaa ctcgcgccag cccgccgaca
     1261 ccgcctgcgg gttgtcccgg tacaggcggt acatctcgtc tacgagaccc gcgttgggcc
     1321 ccagctcctc gaggttcgag gtcatcggtc ggcctgcctt cgggcgcgct tcatcaacac
     1381 catcatcgtg taccacaccg ggaatctgag aaagtcccgc ccgcggtccg gccggccagg
     1441 tcgttcaccc cgcgaattcc accccggagg actacgtcag ctcgggtgta gaaccgatgg
     1501 ggagcggtac agcggcgtcg tggaatatct gctcctcgcc gcggccgccg cagtcgtcgt
     1561 cgtgctgctg ctcggcgtct tcacctacca actccaccgc cgcggctggt gagcaacagg
     1621 acacgaccgg ccgccgcgag ttagcgccgc acgagctcgg agacgcggaa ggccaggtcg
     1681 agcgactggc ggccgttgag gcgcgggtcg cactgggtct cgtagcggcc cgcgaggtgc
     1741 tcgccccgga tctcctcggc gccgccgagg cactcggtga cgtcgtcgcc ggtcatttcg
     1801 atgtgcaccc cgccgggcca cacgccttcg gccttgcaga cgtcgaagaa ctgctcgacc
     1861 tcgcgcaaca cgtcgtcgaa gtggcgcgtc ttgtagctct cgtgcgagaa ggtgttgccg
     1921 tgcatcgggt cgcacgccca cacgaccggg tgtccggcgc gcttcaccgc gcgcagcaac
     1981 gggggcagcg cttcggcgac gcgcgttgcg cccatgcgcg agatcaacgt gaggcgcccg
     2041 gggtcgcgac cggggtcgag gcgccggcac aacgcgagca cctgttcggg ggtcgcatcg
     2101 gggccgagct tcaacccgat cgggttctgc acgcccgaca agaactcgac gtgggccccg
     2161 tcgatctggc gggtgcgctc gccgatccac agcaggtgcg cggagcagtc gtaccagtcg
     2221 ccggtgaggc tgtcgcggcg cgtcaacgct tcctcgtaac cgagcagcag cgcgtcgtgc
     2281 gacgtgaaga agtcgacctc gtgcagctgg aactcggcct ggaggtcgat gccgcacgcg
     2341 gccatgaagc gcatcgcgcg gt
//
LOCUS       DASUGA010000310         2222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1936098, whole genome shotgun sequence.
ACCESSION   DASUGA010000310 DASUGA010000000
VERSION     DASUGA010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1936098"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VFX21_06085"
     CDS             complement(<1..902)
                     /locus_tag="VFX21_06085"
                     /inference="COORDINATES: protein motif:HMM:NF014695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEX5095561.1"
                     /translation="MKVPVPKKLPRPHQVVFALGVLMFFFVLASGLIPVITGWHDDSP
                     IQREVFVNVPDAVVVAFYFSVATMLMIVAWLASLRMKNYERGRPDNRRTNRKNVEKRA
                     KSYRYGVWMQSLLRDPAAGLMHSFLYFGFVVLFIVTVISEIDHQLPEQLKFLHGQTYQ
                     AYSAGAEVAGLAFLIGVVWAIERRFVQRPYRIKIKTKPEDIVIIVTFLVIGVTGFLAE
                     GARIATEHRPNFEKWSFVGYPISALFDDWGPSTISNFHRWMWAVHIVAFLVFLAILPT
                     TKLRHMFTSPMNMYMKDKDRPKGA"
     gene            complement(940..1365)
                     /locus_tag="VFX21_06090"
     CDS             complement(940..1365)
                     /locus_tag="VFX21_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5095562.1"
                     /translation="MARRDAFIDHLQQVPLFAACSRKDLQLVARRAEDVKVPAGKTLV
                     SEGETGHEFFVILEGNARVSRQGRKVATIGPGAAFGELALLEKAPRNATVVADSDMEL
                     VVLGQREFAGLIDEVPGFARKLLAGMAHRLREADSKPVQ"
     gene            1512..>2222
                     /locus_tag="VFX21_06095"
     CDS             1512..>2222
                     /locus_tag="VFX21_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX5095563.1"
                     /translation="MSKAVGIDLGTTNSVVSVLEAGEPAVIPNAEGGRTTPSVVAFSK
                     TGEVLVGEVAKRQAITNPDRTIRSVKRHMGTGWTIDIDGKAYTAQEISARILQKLKRD
                     AEAFLGDTVNQAVITVPAYFDDAQRQATKEAGEIAGLEVLRIINEPTAAALAYGLDKE
                     GSDQTILVFDLGGGTFDVSLLEIGEGVFEVKSTHGDTKLGGDDWDQRVIDWLVDAFKA
                     AHGVDLGKDNMALQRLKEA"
BASE COUNT          420 a          711 c          725 g          366 t
ORIGIN      
        1 atcgcgccct tgggccggtc cttgtccttc atgtacatgt tcatcggtga cgtgaacatg
       61 tggcgcagct tggtggtagg caggatcgcg aggaacacga ggaacgcgac gatgtggacg
      121 gcccacatcc accggtggaa gttcgagatg gtgctcggac cccagtcgtc gaagagtgcc
      181 gaaatcgggt agccgacgaa ggaccacttc tcgaagttgg gccggtgctc ggtcgcgatg
      241 cgcgcgcctt cggcgaggaa gccggtgacg ccgatgacga ggaacgtcac gatgatgacg
      301 atgtcttcgg gcttcgtctt gatcttgatg cggtagggcc gctgcacgaa gcggcgttcg
      361 atcgcccaca ccacaccgat gaggaatgcg agcccggcaa cttcggcgcc ggccgagtac
      421 gcctggtacg tctggccgtg taggaacttc aactgctccg gcagctggtg gtcgatctcg
      481 ctgatcaccg tcacgatgaa cagcaccacg aagccgaagt agaggaacga gtgcatgagc
      541 ccggcggcgg ggtcgcgcag cagcgactgc atccacacgc cgtagcggta gctcttcgcg
      601 cgtttctcga cgttcttgcg attggtccgc cggttgtcgg gtcggccgcg ctcgtagttc
      661 ttcatgcgca gcgaggccag ccacgccacg atcatgagca tcgtggcgac cgagaaatag
      721 aacgccacga cgacggcatc tggaacgttg acgaagacct cgcgctggat cggtgagtcg
      781 tcgtgccagc ccgtgatgac cgggatcagg ccactcgcca gcacgaagaa aaacatcagc
      841 acgccgagcg cgaagacgac ctgatgcggg cgcggcagct tcttcggaac cggaaccttc
      901 atcgaggcgg aatctacaag tacagggccc gaaagcgggc tattggaccg gcttggaatc
      961 cgcttcgcgg aggcgatgcg ccataccggc gagcagcttg cgtgcgaaac ccgggacttc
     1021 gtcgatgagg ccggcgaact cgcgctggcc gagcaccacc aactccatgt cggagtcggc
     1081 gacgacggtg gcgttgcgcg gggccttttc gaggagcgcc agctcgccga acgcggcgcc
     1141 ggggccgatg gtggcgacct tgcggccctg gcggctgacc cgggcgttcc cctccaggat
     1201 cacgaagaac tcgtgcccgg tctcaccctc cgagaccagg gttttcccgg ccggaacctt
     1261 cacgtcctcg gcccggcggg ccacgagctg gaggtccttg cgggagcagg ccgcgaacag
     1321 cgggacctgc tggaggtggt cgatgaatgc gtcacgccgc gccatgggcc cggaatctaa
     1381 ccactttgcg cagtcgccgc acgcgccact ccagtcccgg agatttctgc cgattctggg
     1441 aatctgacaa tggtgttgtc aagttatagt ggtccgaacc actcaactct gtttcttccg
     1501 gaggcacctc tatgtccaag gctgtcggca tcgacctcgg caccacgaac tccgtcgttt
     1561 cggtgctcga ggccggggag cccgctgtta tccccaacgc cgagggcggc cgcacaaccc
     1621 cctcggtcgt cgccttttcc aagaccggcg aggtcctcgt cggcgaggtc gccaagcgcc
     1681 aggcgatcac caaccccgat cgcacgatcc gttccgtcaa gcgccacatg ggcaccggct
     1741 ggacgatcga catcgacggc aaggcgtaca ccgcgcagga aatcagcgcc cgcatcctgc
     1801 agaagctgaa gcgcgacgcc gaggcgtttc tcggcgacac ggtgaatcag gccgtcatca
     1861 ccgttcccgc gtatttcgac gacgcccagc gccaggccac caaggaggcc ggcgagatcg
     1921 caggcctcga agtgctgcgc atcatcaacg agccgaccgc ggctgcgctc gcgtacggcc
     1981 tcgacaagga aggcagcgac cagacgatcc tcgtcttcga cctcggtgga ggcacgttcg
     2041 acgtgtccct gctggagatc ggcgagggcg tcttcgaggt caagtcgacc cacggcgaca
     2101 ccaagctcgg cggcgacgac tgggaccagc gagtcatcga ctggctcgtc gacgcgttca
     2161 aggccgcgca cggcgtcgac ctgggcaagg acaacatggc gttgcagcgc ctgaaggaag
     2221 cc
//
LOCUS       DASUGA010000311         4417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_914607, whole genome shotgun sequence.
ACCESSION   DASUGA010000311 DASUGA010000000
VERSION     DASUGA010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914607"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /gene="hisG"
                     /locus_tag="VFX21_06100"
     CDS             complement(<1..830)
                     /gene="hisG"
                     /locus_tag="VFX21_06100"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX5095564.1"
                     /translation="MLRLVLPKGSLERATLQLFEDADLAVVRSSDVDYRATIDDPRVI
                     DVTILRPQEIPRYVADGLFDVGVTGRDWIEETGVEVATLSELHYSKASSRPARIVLAV
                     AADAQWQTVKDLPEGVRVHTEYPEMTRRFLEKNGVRADIALSYGATEAKIPDIADVVV
                     DLTETGRALRAAGLRIIDTIVVSYTELIANPVSYADADKRKAMEQLRTLLSGALEARG
                     RVLVKLNVDGANLEKVIDLLPALKSPTVSKLHGEDGYAVETVVAKAEINVLIPALKDH
                     G"
     gene            complement(837..1586)
                     /locus_tag="VFX21_06105"
     CDS             complement(837..1586)
                     /locus_tag="VFX21_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009719702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5095565.1"
                     /translation="MSVSYDRIGRSYTTTRRADPRIEAQIHGALGDAASVVNVGAGAG
                     SYEPRDRRVVAVEPSATMIAQRAPDASPAMRAVAEALPFPDRSFDTALGVLTMHHWSD
                     VAAGLRELRRVARRQVLMLFEPSFADQAWIVADYFPEMVDMETERNAPSVDDLARVLD
                     VHHVEPVLVPGDCLDGFGGAYWNRPEEYLRPEVQAGMSCFAQMDPDVLARGMDRLRME
                     LETGHWDAKYGALRAMSATDLGYRLVVAGLP"
     gene            complement(1583..2005)
                     /gene="ribH"
                     /locus_tag="VFX21_06110"
     CDS             complement(1583..2005)
                     /gene="ribH"
                     /locus_tag="VFX21_06110"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX5095566.1"
                     /translation="MRIAIAVARFNRDITEQLLDGAEKTLRKLGAAQVDVYWVPGAYE
                     LPVTAHRLASGDGVDAVIAIGAVIRGETAHFEYVAGECASGLTRASLDTGKPVIFGVL
                     TVDTHQQALDRVGGKDGHKGEEAAETAVEMVSLLRALP"
     gene            complement(2053..3291)
                     /locus_tag="VFX21_06115"
     CDS             complement(2053..3291)
                     /locus_tag="VFX21_06115"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016691693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEX5095567.1"
                     /translation="MSNEAVEQAIDAVRRGEFVVVVDDEDRENEGDLIIAAEKMTPEK
                     MAFMIRYTSGVICLPMEGTRLDALQLPLMVSGSENTEGQRTAFTVSVDAKAGTTTGIS
                     AADRCATVRTLIDPATTSDDLARPGHIFPLRYREGGVLKRAGHTEAAVDFARLAGCFP
                     AGVLAEIVNDDGTMQRRADLEAFAAEHGLVLVTIADLIRYRNHREKLVRRISEARIPT
                     KHGDFTAHVFESLLDGAEHLAFVRGEVAGKKNVLVRVHSECLTGDVFGSLRCDCGTQL
                     DEAMARVAEAGEGVVVYLRGHEGRGIGLGHKLRAYSLQDQGRDTVEANIELGFPPDSR
                     EYGIGAQILVDLGVTTMRLMTNNPAKYGGIDGYGLEIVDRVPLHVEPNHENIAYLRTK
                     QEKLGHLLELVDDDEHLGTS"
     gene            complement(3294..3902)
                     /locus_tag="VFX21_06120"
     CDS             complement(3294..3902)
                     /locus_tag="VFX21_06120"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019356181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX5095568.1"
                     /translation="MFTGIVEELGRVRAITPNEGGARVEIDAKLVLEDAQLGDSIAVN
                     GCCLTVVDLADGWWAADAVTETLARTNLGALAAGDGVNLERPVRLADRLGGHLVQGHV
                     DATGTVAAREKVADGSELVRFHAPAGVMRYVVHKGSITVDGISLTVAALHDDGFTIAV
                     IPHTLAVTTLGARAVGEPVNLEVDVVAKYVERLLTPLDPKEQ"
     gene            complement(3903..>4417)
                     /locus_tag="VFX21_06125"
     CDS             complement(3903..>4417)
                     /locus_tag="VFX21_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HEX5095569.1"
                     /translation="ALTVRDVATPPRIAPTRVLLDARGRVPATGPLFDADLAPTLVLT
                     TTAAPPDAIDAWTHAGAKVEVVAPGADGTGVDLDAVFALLGGGREGFVQAMIEGGGTL
                     LGAVLDAGHAQRLVAYVAPILLGERGTPGYRLDGPTTLAAARRFALTSVRRLGPDVRL
                     DYDLTDRGAV"
BASE COUNT          634 a         1522 c         1561 g          700 t
ORIGIN      
        1 ccgtggtcct tgagcgcggg gatcaacacg ttgatctccg ccttggcgac gacggtctcg
       61 accgcgtagc cgtcttcgcc gtgcagcttc gataccgtcg gcgacttcaa cgccggcagg
      121 aggtcgatga ccttctccaa gttggcaccg tcgacgttga gcttgacgag cactcggccg
      181 cgcgcttcga gcgcgcccga cagcaacgtg cggagctgct ccatcgcctt gcgcttgtcg
      241 gcgtccgcgt acgacaccgg gttcgcgatc agctcggtgt acgagacgac gatcgtgtcg
      301 atgatccgca gccccgcggc gcgcagcgcg cggccggtct cggtgaggtc gacgacgacg
      361 tccgcgatgt cggggatctt cgcctcggtc gcgccgtagg agagggcgat gtcggcacgc
      421 acgccgttct tctcgaggaa gcggcgagtc atctcgggat actcggtgtg gacgcgcacg
      481 ccctcgggca gatccttgac cgtctgccac tgcgcgtcgg cggcgaccgc gagcacgatg
      541 cgcgccggac gcgagctcgc cttcgagtag tgcagctccg acagcgtcgc gacttcgacg
      601 ccggtctcct cgatccagtc gcggccagtg acacccacgt cgaagagccc gtcggccacg
      661 taacgcggga tctcctgcgg gcgcaggatc gtcacgtcga tgacccgggg gtcgtcgatc
      721 gtggcccggt agtcgacgtc ggaggaccgc acgaccgcga ggtcggcgtc ctcgaacagc
      781 tggagcgtgg cccgctccaa cgatcccttc ggcaagacga ggcgcaacac ggctgatcag
      841 ggtagcccgg caaccacgag gcgatagccg agatccgttg ccgacatggc gcgcagcgcg
      901 ccgtacttcg cgtcccagtg gccggtttcg agctccatcc gcagccggtc catgccgcgc
      961 gcgagcacgt cggggtccat ctgcgcgaag cacgacatgc ccgcctgcac ctcgggccgc
     1021 aggtactcct cgggccggtt ccagtaggcg ccgccgaagc cgtcgaggca gtcgccgggc
     1081 acgaggaccg gttcgacgtg gtgcacgtcg aggacgcgcg cgagatcgtc gacgctcggc
     1141 gcgttgcgct cggtctccat gtcgaccatc tcggggaagt agtcggcgac gatccatgcc
     1201 tggtcggcga aggacggctc gaacaacatg agcacctgcc gtcgtgcgac gcggcgcagc
     1261 tcgcgcaggc cggccgcgac gtcggaccag tgatgcatcg tcagcacacc gagcgcggtg
     1321 tcgaagctcc ggtcggggaa cggcagcgcc tcggcaaccg cacgcatggc gggcgaggca
     1381 tcgggcgcgc gctgggcgat catcgtggcc gacggttcga cggcgaccac gcgccggtcg
     1441 cggggctcgt agctccccgc gccggcaccg acgttgacga ccgacgcggc atcgcccagg
     1501 gcaccgtgga tctgggcttc tatgcgcggg tcggcccgcc gggtggtcgt gtacgaacga
     1561 ccgatccggt cgtaggaaac gctcacggga gcgcgcgcag cagcgagacc atctcgaccg
     1621 cggtctccgc ggcttcttcg cccttgtggc cgtccttgcc gccgacgcgg tcgagcgcct
     1681 gctggtgcgt gtcgacggtg aggacaccga agatcaccgg cttcccggtg tcgagcgagg
     1741 cgcgcgtcaa gccgctcgcg cactcccccg ccacgtactc gaagtgcgca gtctcgccgc
     1801 gaatgaccgc gccgatcgcg atcaccgcgt cgacgccgtc gccggacgcg agtcggtgcg
     1861 ccgtcaccgg cagctcgtac gcgcccggga cccagtacac gtcgacctgt gccgcgccga
     1921 gcttgcgcag cgtcttctcc gcgccgtcga gcagctgttc ggtgatgtcg cggttgaagc
     1981 gcgcgactgc gatcgcgatg cgcatcccgc tcgcgtcgag gtcaccttcg tgcgccgcgt
     2041 actcgcccac ggttagctcg tccccagatg ctcgtcgtcg tcgaccaact cgagcaggtg
     2101 gccgagcttt tcttgcttgg tgcgcaggta cgcgatgttc tcgtggttcg gttcgacgtg
     2161 cagcggcacg cggtcgacga tctccagccc gtagccgtcg atgccgccgt acttcgccgg
     2221 gttgttcgtc atcaaccgca tggtcgtcac gccgaggtcg accaggatct gcgcgccgat
     2281 gccgtactca cgcgagtcgg gcgggaagcc gagctcgatg ttcgcttcga cggtgtcgcg
     2341 gccttggtcc tgcagcgaat acgcccgcag cttgtggccg aggccgatgc cgcggccctc
     2401 gtggccgcgc aggtagacaa cgacgccttc acccgcttcg gcgacgcgtg ccatcgcttc
     2461 gtcgagctgc gtgccacagt cgcagcgcag cgaaccgaac acgtcgcccg tgaggcactc
     2521 ggagtgcacg cgcaccaaca cgttcttctt gcccgcgacc tcgccccgga cgaacgccaa
     2581 gtgctcggcg ccgtcgagga gcgattcgaa gacgtgggcg gtgaagtcgc cgtgcttcgt
     2641 ggggatgcgt gcctcggaga tgcggcgcac gagcttctcg cggtggttgc ggtagcggat
     2701 gaggtcggcg atcgtcacga gcacgaggcc gtgctcggcc gcgaacgctt cgaggtcggc
     2761 gcggcgctgc atcgtgccgt cgtcgttcac gatctccgcg agcacaccgg ccgggaagca
     2821 accggccagc cgcgcgaagt cgaccgccgc ttcggtgtgc ccggcgcgct tgagcacgcc
     2881 gccttcgcga tagcgcaacg ggaagatgtg gccgggccgc gcgaggtcgt cggatgtcgt
     2941 cgcgggatcg atcagcgtgc gcacggtcgc gcagcggtcg gccgcggaga tgcccgttgt
     3001 ggtgccggcc ttggcgtcga cggacacggt gaacgcggtg cgttggcctt cggtgttctc
     3061 gctgcccgag accatgagcg gcagttgcag cgcatcgaga cgggtgccct ccatcggcag
     3121 gcagatgaca ccgctcgtgt agcggatcat gaacgccatc ttctcgggcg tcatcttctc
     3181 ggccgcgatg atgaggtcgc cttcgttctc gcggtcctcg tcgtcgacga ccacgacgaa
     3241 ctcgccgcgg cgtacggcgt cgatggcctg ctcgaccgct tcgttggaca tgctcattgc
     3301 tccttcgggt cgagcggcgt gagcagccgc tccacgtact tggcgacgac gtcgacttcg
     3361 aggttcaccg gctcgccgac ggcgcgggct ccgagtgtcg tcaccgcgag cgtgtgcggg
     3421 atgacggcga tcgtgaatcc gtcgtcgtgc aacgcggcaa cggtgaggct gatgccgtcg
     3481 acggtgatcg aacccttgtg caccacgtag cgcatgacgc ccgcgggtgc atggaaccgg
     3541 acgagctcgg aaccgtccgc caccttctcg cgcgcggcga cggtgccggt cgcgtcgacg
     3601 tggccctgga cgaggtggcc accgagccgg tcggcgagcc gcaccgggcg ttcgaggttc
     3661 acgccgtcac cggccgccag cgcgccgagg ttcgtgcgcg ccaacgtctc ggtcaccgcg
     3721 tcggccgccc accaaccgtc ggcgaggtcg acgacggtga ggcagcagcc gttgaccgcg
     3781 atggagtcac cgagctgcgc gtcttcgagc acgagcttgg cgtcgatctc gacgcgcgcg
     3841 ccgccctcgt tcggcgtgat cgcgcgcacg cggcccaact cttcgacgat gccggtgaac
     3901 atctacacgg ctccccggtc ggtgaggtcg tagtcgaggc ggacgtcggg gccgagccgg
     3961 cgcacgctcg tcagcgcgaa acggcgggcg gcggcgagtg tcgtgggacc gtcgaggcgg
     4021 tagccgggcg tgccgcgctc gccgagcagg atcggcgcga cgtatgcgac cagtcgctgc
     4081 gcgtgcccag cgtcgagcac ggcgccgagc aacgtgccgc cgccctcgat catcgcctgc
     4141 acgaatcctt cgcgcccgcc gccgagcagc gcgaacacgg cgtcgaggtc gacgcccgtg
     4201 ccatcggcgc ccggcgcgac gacttcgacc ttggcacccg cgtgcgtcca cgcgtcgatg
     4261 gcgtcgggtg gcgcggcggt tgtcgtgagc acgagcgtcg gcgcgaggtc ggcgtcgaac
     4321 agcggtccgg tcgcgggcac acggccacgc gcatcgagga ggacgcgcgt cggagcgatg
     4381 cgcggcggcg tcgcaacatc gcgcacggtc agcgcgg
//
LOCUS       DASUGA010000312         3221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1853977, whole genome shotgun sequence.
ACCESSION   DASUGA010000312 DASUGA010000000
VERSION     DASUGA010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1853977"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..792
                     /locus_tag="VFX21_06130"
     CDS             274..792
                     /locus_tag="VFX21_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095570.1"
                     /translation="MSVALAGMARNAGASDFRPGPRVTGLIGDQYVPWNPQPAKPAKF
                     AVVLLSWSDGPAAPWPASRLRNVLTGGGRSVERHLAEASNGVVRFRTAQNTPGVDIFG
                     WYQLPYSSTSEASCDDNRLQADAIAKAKGVNLDVYDKVAIVVPSQHCFWAGLGDYYGG
                     KWLWISLPPVVT"
     gene            838..1422
                     /locus_tag="VFX21_06135"
     CDS             838..1422
                     /locus_tag="VFX21_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095571.1"
                     /translation="MGHSQRLVCRNKQGGQVAFSAKCEDAWFEPWDPMGAGAYNYSAL
                     HQAQAGFLPSRNVRVVDRSQTIYLRAVERRTATGAHLLLIPTDDSAVRFYAVEFHVAA
                     GAISNFPANSAATRGLMIRRTGLGDVDTKLLDLRPGTPGVLKPRVVQAPNDAFADGTL
                     RPGSTFTDPVSKRSIKLLSYKDGIAIVHVSIPKE"
     gene            1452..2642
                     /locus_tag="VFX21_06140"
     CDS             1452..2642
                     /locus_tag="VFX21_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014060268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5095572.1"
                     /translation="MQTVAIVGMSLAGLRAAETLRREGYDGRIVAIGGESHLPYDRPP
                     LSKEILRGEWEPDQIVLRKQGVEDLELDWRLGARATGLDVAARTVELHDGEQVAFDGL
                     VIATGAVPRRMPNQPDLAGVFVLRSLDEALALRECLTGNPRVVVIGAGFIGAEVAAAC
                     RMRGLDVTVLEMLPQPMVRGLGPDLGATIAAVHRDHGVDLRTGVTVEEIVGADGEVRA
                     VRLGDGTVIEADVVVVGVGVVPETGWLEGSGLKIDNGIVCDERCEASPGVVAAGDVAR
                     WPNPLFDGASMRLEHWTNATEQGVHAARRLLGHDEPFAPVPFVWSDQYDRKIQTVGIV
                     SADADTHVAHGTLADRQFVSLFGRDGRIVGALGFNRPRNVMQYRRLIAERASWDDALA
                     LANS"
     gene            2639..>3221
                     /locus_tag="VFX21_06145"
     CDS             2639..>3221
                     /locus_tag="VFX21_06145"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5095573.1"
                     /translation="MSDAKHKLLSLRFVTVVTCGTFYFIALAMLTPVIPHYVKDDLGQ
                     GDFAVGIATGVFAVGAILLRLYFGRLGDRLGRRVLIIFGASVVAVTTLMYGAVDSLAW
                     LIVWRFATGFGEAAFFVGAATMVTDLAPVERRGEAISYWSIAVYSGLAFGPALGELLR
                     GTDRYVLTFTVSAALAGVAALLGLFTRDVPREPS"
BASE COUNT          489 a         1049 c         1115 g          568 t
ORIGIN      
        1 aacgcgtcga tgtcaacgtc gcgcagacgc agtggacggc cggagacgtc gagcggtggg
       61 ggcgtgggca ttgggcactc cggatcgggc ggcccgccag tatgacgtga gcgtcaggaa
      121 cccagccatt cgcgctgggc cgatcgactc atttcgccgg tcgcgtaaac cgtcctcgaa
      181 cggtctgcgt cagtcaagca agaccggcga ccagccgccg gcgacgggcg gaacaggcgg
      241 aacatgcggc gttgcatcgt gggactcatt tgcttgtccg tcgcgctcgc gggcatggcg
      301 cgaaatgccg gcgcctctga cttccggcca gggccgcgag tcaccggtct gatcggcgac
      361 cagtacgtgc cgtggaaccc gcagcctgcg aagccggcca agttcgcagt cgttctcctt
      421 tcgtggagtg acggacccgc ggcgccgtgg cccgcgagcc ggctccgaaa cgtgttgacc
      481 ggcggcggcc gctcggtcga gcgccacctt gccgaagcga gcaacggcgt cgtgcggttc
      541 cgcaccgcgc agaacacacc cggcgtcgac attttcgggt ggtaccagtt gccgtactcg
      601 tcgacgtccg aggcgagctg tgacgacaac cggttgcaag ccgacgccat tgcgaaggcg
      661 aagggtgtca atctcgacgt ctacgacaag gtcgccatcg tcgtgccgtc gcaacattgc
      721 ttttgggcgg gcctcggcga ttactacggc gggaagtggc tctggatctc gctcccaccg
      781 gtggtgacct agtactggta cagcattttc gtacacgagc tcggacacac ctaccgcatg
      841 ggccactcgc agcggctggt gtgccggaac aagcaaggcg ggcaggttgc gttttccgcg
      901 aagtgtgaag acgcgtggtt cgagccctgg gatccgatgg gcgccggcgc gtacaactat
      961 tccgcgcttc atcaggctca agcgggcttt ctcccaagtc gcaatgttcg tgtcgtcgac
     1021 cggtcgcaga cgatctatct ccgcgccgtc gaacgcagga ccgcgaccgg ggcgcatctg
     1081 ctgctgatcc caacggatga cagcgcggtt cggttctacg cggttgagtt ccatgtcgcg
     1141 gcgggcgcca tctccaactt tccggcaaac agcgcggcga cgcgcggcct catgatccgc
     1201 cggaccggac tcggcgacgt cgacacgaag ttgctcgatc tccggcccgg cacgcccggc
     1261 gtgttgaagc cgagagtggt gcaggcgccg aacgacgcgt ttgccgacgg cacgctgcgg
     1321 cccggttcca cgttcaccga tccggtgagc aaacggtcga tcaagctgct ctcgtacaaa
     1381 gacggcatcg cgatcgtgca cgtctccatt ccgaaggagt gagtcgcgcg gcccttcggt
     1441 agggtcggcg ggtgcaaacg gtcgcgatcg tcggtatgtc cctcgcgggt ctgcgcgcgg
     1501 ccgagacgct ccgacgtgag ggctatgacg ggcgcatcgt cgcgattggt ggcgagtcgc
     1561 acttgccgta cgaccgtccg ccgctctcga aggagatctt gcgcggcgag tgggagcccg
     1621 accagatcgt gttgcgcaag cagggcgtcg aagatttgga gctcgactgg cggctcggcg
     1681 cgcgagcgac tgggctcgac gttgcggcgc gcacggtcga actccacgac ggcgagcagg
     1741 tggcgttcga cggcctcgtg atcgcgaccg gcgcagtgcc gcggcgcatg ccgaaccagc
     1801 ccgaccttgc gggtgtgttc gtgctgcgtt cgctcgatga ggcgctcgcg ctgcgcgagt
     1861 gcctgaccgg caatcctcgc gtcgtggtca tcggcgcggg attcatcggg gccgaagtcg
     1921 ccgccgcgtg ccggatgcgt ggcctcgacg tgacggtgct cgaaatgttg ccgcagccga
     1981 tggtgcgcgg tctcgggcct gatctcggcg ccacaattgc cgcagtgcat cgggatcacg
     2041 gcgtcgacct gcgcaccggt gtcaccgtgg aggagattgt cggcgccgac ggcgaagtgc
     2101 gcgcagtccg gctcggcgac ggaaccgtta tcgaagccga cgtcgtcgtg gtcggggtcg
     2161 gcgtcgtgcc cgaaaccggg tggctcgagg gatccggact caagatcgac aacggcatcg
     2221 tctgcgacga acgctgcgag gcctcgccgg gcgtggtcgc cgccggcgac gtcgcgcgct
     2281 ggcccaaccc gctgttcgac ggcgcgtcga tgcgactcga gcactggacg aacgcaaccg
     2341 aacagggagt gcacgccgcg cggcggctgc tcggccacga cgaaccgttc gcgccggtgc
     2401 cgttcgtgtg gtcggatcaa tacgaccgca aaatccaaac cgtcggaatc gtctcggccg
     2461 atgccgacac gcatgtcgcg cacggcacgc tcgccgaccg ccagttcgtg tcgctgttcg
     2521 ggcgcgacgg ccgcatcgtt ggtgccctcg gcttcaaccg cccgcgcaac gtgatgcaat
     2581 accggcggct gatcgccgag cgggcgtctt gggacgacgc gctcgcgctc gcgaactcgt
     2641 gagcgacgcc aagcacaagc tgctgtcgct ccggttcgtc accgtcgtga cgtgcggaac
     2701 cttctacttc atcgcgctcg cgatgttgac gccggtgatt ccgcactacg tcaaggacga
     2761 cctcggccag ggcgacttcg cggtgggcat tgccaccggc gtcttcgcag tcggcgcgat
     2821 cctgttgcgc ttgtacttcg gccgcctcgg cgaccggctc ggcagacgag tgctcatcat
     2881 cttcggcgcg tcagtggtcg cggtcacgac cttgatgtac ggcgcggtcg actcgctggc
     2941 ctggctcatc gtgtggcggt tcgcgaccgg gttcggcgaa gcggcatttt tcgtgggcgc
     3001 cgcgacgatg gtcacggacc ttgcacccgt cgagcggcgg ggtgaagcga tctcgtactg
     3061 gtcgatcgcg gtgtacagcg gcttggcgtt cgggcccgcc ctcggcgagt tgttgcgcgg
     3121 caccgaccgg tacgtcctta ccttcaccgt ttccgccgcc cttgccggtg ttgccgcact
     3181 gctcgggctg ttcacgcgcg acgtgccgcg cgagccctcg a
//
LOCUS       DASUGA010000313         4695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1495825, whole genome shotgun sequence.
ACCESSION   DASUGA010000313 DASUGA010000000
VERSION     DASUGA010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495825"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VFX21_06150"
     CDS             <1..658
                     /locus_tag="VFX21_06150"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX5095574.1"
                     /translation="LVEEYIEHNERARAEVDELRETAAALALMPSGEGSAPAELWDRI
                     AADIAPTDELRARREKKPRHWVAPVLAVAAAVIAIVLTVQVVSLDGRLGDARDDQGNV
                     AIAFDRALEADGARTTELVSADTNTPVGRIVVLPDGTGYMKSDDMAALSDDETYQLWA
                     LVGNETDRRVISAGVLGNDPGAAGFRIDGPVEGFAVTIEQAGGVEESANDPIAIGSLA
                     "
     gene            669..1769
                     /gene="hemW"
                     /locus_tag="VFX21_06155"
     CDS             669..1769
                     /gene="hemW"
                     /locus_tag="VFX21_06155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX5095575.1"
                     /translation="MGYGVYVHIPFCAHRCDYCDFATWTDRAHLIDDYVDACITDLGR
                     RDAPTATSVFFGGGTPSLIEGDQLARIVAAIDVTSDAEITVECNPDSVDADKLHAYKD
                     AGVNRLSFGVQSFAPHVLRALNRTHDPANVERAIALARAAGFDNVNADIIYGTAGESA
                     DDWRRTVDHVIALGVEHVSAYALTVEPPTPLGQRVAAGTAAAPDDDDQADKYVVADEQ
                     LIAAGYGWYEISNWARPGRECRHNQLYWEQGDYLAIGCAAHGHTTGRRWWNVRTPERY
                     IERIAANESPEAGHETLAAETRADEAFTLALRTRSGAPVPPGTGAVVADLVAGGLLEP
                     APDGRVVCTRAGRLVANDLTARILAAAVSTGR"
     gene            1821..2861
                     /gene="hrcA"
                     /locus_tag="VFX21_06160"
     CDS             1821..2861
                     /gene="hrcA"
                     /locus_tag="VFX21_06160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX5095576.1"
                     /translation="MPQEVPQASSSRLDERKAAVLRAIIEQYVATGQPVGSATVTRAA
                     ELGVSAATVRNEMSTLERDGFIGQPHTSAGRVPTDQGYRYYVDHLAGTAQLSTPDRRR
                     IAEFFTSATMAMDELLAETSQLLARVSAHAALVVGPQSDAVVVKGAHLTQIQPRVLLA
                     VVILSNGAVEKEIVMLDHDVADNDVAAASAQLAKVLDGRRLGDLPPTAAAAAGDVAGH
                     LVRASCDALRIHLESHNREPLYVGGASRLAAEQDAFTATTISRVYELLEQHVVLASVM
                     RELLGPGLTVRIGSENERTDLRECSLVLAPYLIDGELRGTIGVLGPTRMDYRKAQAAV
                     TAVSQQLGRQLS"
     gene            2858..3973
                     /gene="dnaJ"
                     /locus_tag="VFX21_06165"
     CDS             2858..3973
                     /gene="dnaJ"
                     /locus_tag="VFX21_06165"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX5095577.1"
                     /translation="MTNYYELLGVTQNATEDEIKKAYRTLARQYHPDANGNDPAAAER
                     FKEIANAYETLSDPERRRRYDMFGSDDAGAGASGFAGAGAFGLNDLFDAFFGGDAFGR
                     MGGAGGGPPRGADVEIGIDLTLSEIVFGARKTIEPRMPVECETCGGSGAAPGTTPQRC
                     PTCEGAGEVRQVRRSLLGQIITASPCPACGGTGEIVPTPCETCRGDGRVNGTRSLDVD
                     VPPGIEDGQRLRLAGRGPAAPRGGQAGDLYVAVRVQPDSRFTRRGDDLWMQQPVSIVQ
                     AALGMHATLDTLDGPHELEIPHGTQHGAQFRVKGLGVPHLRSGRRGDLVCEIAVEVPT
                     HLTHEEAELLAQFAALRGEDVQPPREGLFSRIKSAFQ"
     gene            3970..>4695
                     /locus_tag="VFX21_06170"
     CDS             3970..>4695
                     /locus_tag="VFX21_06170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEX5095578.1"
                     /translation="MTFAAGVAVPAHLFVDALADECTVDGRDGHHLQRVRRLRVGEHV
                     TAADGDGAWRRYEIVASAGGQLELAARGAIEHEPDVQPRVGLALALTKGGIDAAIARA
                     TELGVAWIEPLEAARSVARFDHGRAEHAYERWRTVVREAAAQSRRARLPEIRPRVRLA
                     DLAVRPGALLADRTGVPAAALPPPPALGWTVVVGPEGGFEPAEVDVLSGMPRLAVGPF
                     VLRAETAPVAAVASLMSQEAPGSV"
BASE COUNT          699 a         1700 c         1663 g          633 t
ORIGIN      
        1 gctcgtcgag gagtacatcg agcacaacga gcgggcccgg gccgaggtgg acgagttgcg
       61 cgagaccgcg gccgcgctcg ccttgatgcc gagcggcgaa ggatcggcgc cggccgagct
      121 ctgggaccgc atcgccgccg acatcgcacc gaccgacgag ctccgggccc ggcgcgagaa
      181 gaagccgcgc cactgggtcg caccggtgct cgccgtcgca gccgccgtga tcgcgatcgt
      241 gctgacggtg caggtcgtct cgctcgacgg ccgcctgggc gacgcgcgtg acgaccaggg
      301 caacgtcgcg atcgcattcg accgcgcgct cgaagccgac ggcgcgcgta cgaccgagct
      361 cgtgagcgcg gacacgaaca cgccggttgg ccgcatcgtc gtgctgcccg acggcaccgg
      421 ctacatgaag agcgacgaca tggccgcgct gtccgacgac gagacgtacc aactctgggc
      481 gctcgtcggc aacgagaccg accggcgcgt catctccgcc ggcgtgctcg gcaacgaccc
      541 cggcgccgcc ggcttccgca tcgacggacc cgtcgaaggc ttcgcggtga cgatcgagca
      601 ggcgggcggc gtcgaggagt cggccaacga cccgatcgcg attggctcgc ttgcctagcg
      661 ttggacccgt gggttacggc gtctacgtcc acatcccgtt ctgtgcacat cgctgcgact
      721 actgcgattt cgcgacgtgg accgatcgcg cccacctgat cgacgactac gtcgatgcct
      781 gcatcactga tctcgggcgg cgagacgcac cgaccgcgac cagtgtcttc ttcggcggcg
      841 gcacgccctc gctgatcgaa ggcgaccagc tcgcccgcat cgtcgccgcc atcgacgtca
      901 cgagtgacgc cgagatcacg gtcgagtgca atcccgacag cgtcgacgcc gacaagctgc
      961 acgcctacaa ggacgccggc gtcaaccggc tgtcgttcgg cgtgcagtcg ttcgcgccgc
     1021 acgtgctccg cgcgctgaac cgcacgcacg accccgcgaa cgtcgaacgg gcgatcgcgc
     1081 tcgcgcgggc agcgggcttc gacaacgtca acgccgacat catctacggc acggccggcg
     1141 agagcgccga cgattggcga cgcaccgtcg accatgtcat cgcgctcggt gtcgagcacg
     1201 tcagtgcgta cgcgctcacc gtcgagccgc cgacaccact cggtcagcgc gtcgccgccg
     1261 gcactgcggc ggcgcccgac gacgacgacc aagccgacaa gtacgtcgtc gccgacgagc
     1321 agctcatcgc ggccggctac gggtggtacg agatctcgaa ctgggcccgc cccgggcgcg
     1381 agtgccggca caaccagctg tattgggaac agggcgacta cctcgcgatc ggctgtgccg
     1441 cgcacgggca caccacgggc cgccggtggt ggaacgtccg cacaccggag cgctacatcg
     1501 aacggatcgc ggcaaacgag tcgcccgaag ccgggcacga gaccctcgcg gccgagaccc
     1561 gcgccgacga ggcgttcacc ctggcgctgc gcacccgatc gggcgcgccc gtgcccccgg
     1621 gcaccggcgc cgtcgtcgcc gacctggtcg ccggcggtct gctcgagccc gcccctgacg
     1681 gccgcgtggt gtgcacgcgg gcggggcgcc tggtcgccaa cgacctcacg gcccggatcc
     1741 tggccgcggc agtgtccaca gggcgctagc actcgttacc attgagtgct aacggaggtg
     1801 cggtcccgcc gcaacattgc atgccgcaag aagtgcctca ggcttcttcg tcccgcctcg
     1861 acgagcggaa ggccgccgtc ctgcgggcga tcatcgagca atatgtcgca accgggcagc
     1921 cggtcgggtc cgcgaccgtg acgcgcgcgg ccgagctcgg ggtctcggcg gcgaccgtgc
     1981 gcaacgagat gtcgacgctc gagcgcgacg gcttcatcgg ccagccgcac acgtcggccg
     2041 gccgggtgcc gaccgaccag ggctaccgct actacgtcga ccacctcgcc ggcaccgcgc
     2101 agctctcgac gcccgaccgg cgccgcatcg ccgagttctt cacctccgcg accatggcga
     2161 tggacgaact gctcgcggag acgagccagc tgctcgcgcg cgtgtcggcg cacgccgcgc
     2221 tcgtcgtcgg cccgcagtcc gatgctgttg tcgtgaaggg cgcgcatctc acacagatcc
     2281 agccgcgtgt gctcctcgca gtcgtgatcc tctcgaacgg cgcggtcgag aaggagatcg
     2341 tgatgctcga ccacgacgtc gccgacaacg acgtcgcggc cgcgagcgcg caactcgcca
     2401 aggtgctcga cggtcgccgg ctcggcgacc tgccaccgac ggcggccgcc gcggcgggcg
     2461 acgtcgcggg gcatttggtc cgggcctcgt gcgacgcatt gcgcatccat ctcgagtcgc
     2521 acaaccgcga gccgctctat gtcggcggcg cgagccggct ggcggccgaa caggacgcat
     2581 tcaccgcgac caccatctcc cgcgtctacg agctgctcga gcagcatgtc gtgctcgcgt
     2641 cggtgatgcg ggagctactc ggccccggcc tgacggtgcg catcgggtcg gagaacgagc
     2701 gcaccgatct gcgtgaatgc tcgctcgtgc tcgcgccgta cctgatcgac ggtgagctgc
     2761 gcggcacgat cggtgtcctc ggcccgacgc gcatggacta ccgcaaggcg caagccgcgg
     2821 tgaccgcggt ctcgcaacaa ctcgggcggc agttgtcgtg acgaactact acgagctcct
     2881 gggcgtcacg cagaacgcca ccgaagacga gatcaagaag gcgtatcgca cacttgcgcg
     2941 ccagtatcac cccgacgcga acggcaacga ccccgcggcc gcggagcggt tcaaggagat
     3001 cgcgaacgcg tacgagacgc tgagtgaccc ggagcgtcgc cgtcgctacg acatgttcgg
     3061 cagcgacgac gccggtgcgg gcgcgagcgg attcgccggt gcgggcgcgt tcggcctcaa
     3121 cgacctgttc gatgcgttct tcggcggcga cgcgttcgga cgcatggggg gcgcgggcgg
     3181 cgggccgccg cgcggtgccg acgtcgagat cggcatcgac ctgacgttgt cggagatcgt
     3241 gttcggcgcg cgcaagacga tcgagccgcg gatgccggtc gaatgcgaaa cgtgcggcgg
     3301 ctcgggcgcg gcgcccggca cgacaccgca gcggtgcccg acgtgcgaag gcgccggcga
     3361 agtccgccag gtgcgccggt cgttgctcgg ccagatcatc accgcgtcgc cgtgcccggc
     3421 atgcggcggg acgggcgaga tcgtgccgac gccgtgcgag acgtgccgcg gtgacggccg
     3481 tgtcaacggc acgcgttcgc tcgacgtcga cgtcccgccc ggcatcgaag acggccagcg
     3541 gttgcggctc gccggccgcg gcccggccgc ccctcgcggc ggccaagccg gcgatctcta
     3601 cgtcgcggtg cgcgtgcaac ccgacagtcg tttcacgcgc cgcggcgacg acctctggat
     3661 gcagcaaccg gtgtcgatcg tccaggccgc gctgggcatg cacgccacgc tcgacacgct
     3721 cgacggtccg cacgagctcg agatcccgca cggcacccag cacggcgcgc agttccgtgt
     3781 gaaaggcctc ggtgtgccgc acttgcgcag cggtcggcgc ggcgacctcg tgtgcgagat
     3841 cgcggtggag gtgccgacgc atctgacgca cgaggaagcc gagctgctcg cgcaattcgc
     3901 cgcactgcgc ggcgaagacg tgcaaccgcc gcgcgagggc ttgttctcgc gcatcaagtc
     3961 cgccttccag tgacgttcgc agcgggcgtt gccgttcccg cgcatctctt cgtcgacgcg
     4021 ctcgcggacg agtgcaccgt cgacggccgc gacggacatc atctccaacg cgtgcgccgg
     4081 ttgcgtgtcg gcgaacacgt cactgcggcc gacggcgacg gcgcgtggcg gcgatacgag
     4141 atcgtcgcgt cggcgggcgg gcagctcgag ctcgcggcgc gcggcgcgat cgagcacgag
     4201 ccggacgtgc agccgcgcgt cgggctcgcg ctcgcgctga ccaagggcgg catcgacgcg
     4261 gcgattgcgc gcgccaccga gctcggcgtc gcgtggatcg agccgctgga ggccgcgcgg
     4321 tcggtggccc ggttcgacca cggccgcgcc gagcatgcct acgagcggtg gcggaccgtc
     4381 gtgcgggagg ccgcggcgca gtcgcgccgg gcccggctgc ccgagatccg gccgcgcgtg
     4441 cgcctcgccg accttgccgt gcgcccgggc gccctcctcg cggaccgcac cggcgtgccg
     4501 gccgccgcgc tcccgccgcc gccggcactg ggctggacag tggtggtcgg tccggagggc
     4561 ggcttcgagc ccgccgaggt ggacgtcttg tcgggaatgc cccgcctggc cgtgggcccc
     4621 ttcgtgctcc gggccgagac cgcgccggtc gcggcggtcg cgagccttat gagccaagaa
     4681 gcccctggca gtgtg
//
LOCUS       DASUGA010000314         2109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_902625, whole genome shotgun sequence.
ACCESSION   DASUGA010000314 DASUGA010000000
VERSION     DASUGA010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902625"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="VFX21_06175"
     CDS             <1..1109
                     /locus_tag="VFX21_06175"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5095579.1"
                     /translation="AGPIATRYFSEHGATVLRVESKTRADFLRTMALADPKNPHGLEG
                     STMYDGLNAGKRSVTLNLKHPDAVALVKRLVVEWADAIEENFAPRAMKNFGLDYDTLV
                     HDKPDLVMVSACLNGQFGPHKDYPGFGGQGSALGGYNAMTGWPDREPVGPFGTITDSL
                     APRYVATAIAAGLHYHRRTGRGCYLDVSQVETALYSLSPWILDYEVDGNLRLREGNNH
                     ADAKLHGAFACADEDGIGDRWVTIACWTDDELARLHAITGPDVAAWTATRTRAEVSAQ
                     LQAVGIEAVPVNDFGDLHDDPQLAARGHFERHTNPFLGDGLYERNGFRLSDAPSGYTA
                     AGPTLGQDNEWVLGEVLGLGRAEVAALMDAGAIE"
     gene            1106..1582
                     /locus_tag="VFX21_06180"
     CDS             1106..1582
                     /locus_tag="VFX21_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5095580.1"
                     /translation="MKVRRVEAGDREWVAEVVRTHFASTRMVSRGRLYEDVSVLDGFI
                     VEGDGRPIGLSLWEERDGDAELVVMVTLNRGSGAGTMLIDAVVEYAREHDWQRLWLIT
                     TNDNVDAIRFYQRAGWDWVGFYRDAVTEARGLKPEMGELGAHGLPIRHEIEFEAPL"
     gene            complement(1584..>2109)
                     /locus_tag="VFX21_06185"
     CDS             complement(1584..>2109)
                     /locus_tag="VFX21_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5095581.1"
                     /translation="RSHERGHAVGRADMHALPFADRTFAAVRADRVVQHVADPHRAFD
                     ELVRVTSGRIVVADPDQSTLSIVVPGAPAALVDAVTRLRAQVGYRNGTFIREVVDTFD
                     ERDDAELTVDEFTLALTNPDDAFGLPTWVDYWRAEGEFTDADVRVWDDAMQRAREAGG
                     FEFALQYVVVAATV"
BASE COUNT          359 a          720 c          731 g          299 t
ORIGIN      
        1 cggccggccc gatcgcgacg cggtacttct ccgagcacgg cgcgaccgtg ttgcgtgtcg
       61 agtcgaagac gcgcgccgac ttcctgcgca cgatggcgct cgcggatccg aagaacccgc
      121 acggcctcga aggctcgacg atgtacgacg ggctcaacgc gggcaagcgg agtgtcacac
      181 tcaacctcaa gcaccccgac gcagtcgcgc tcgtgaagcg actcgtcgtc gagtgggccg
      241 acgcgatcga ggagaacttc gcgccgcgcg cgatgaagaa cttcggcctc gactacgaca
      301 cgctcgtgca cgacaagccc gatctcgtga tggtcagtgc gtgtctcaac ggccagttcg
      361 ggccgcacaa ggactacccc ggcttcggcg gccagggttc tgcgctcggc ggttacaacg
      421 cgatgacggg ctggcccgat cgcgagccgg tcgggccgtt cggcacgatc accgactcgc
      481 tcgcgccgcg ctacgtcgca accgcgatcg cagccggcct gcactaccac cggcgcaccg
      541 gccggggctg ctacctcgac gtcagccagg tcgagaccgc gctgtacagc ctctcgccgt
      601 ggatcctcga ctacgaagtc gacggcaacc tgcgcctgcg cgagggcaac aaccacgccg
      661 acgcgaaact gcacggtgcg ttcgcgtgcg ccgacgaaga cggcatcggc gaccggtggg
      721 tcacgatcgc gtgctggacc gacgacgagc tcgcacggct gcacgccatc accggtcccg
      781 acgtcgcggc ctggacggcg acccggacac gcgccgaggt cagcgcgcaa ctgcaggcgg
      841 tcgggatcga agccgtcccc gtcaacgact tcggcgacct ccacgacgac ccgcaactcg
      901 cggcgcgcgg tcacttcgag cggcacacca acccgttcct cggcgacgga ctctacgagc
      961 gcaacggctt ccgtctctcg gatgccccga gcggatacac ggccgccggg cctacgctcg
     1021 gacaggacaa cgagtgggtc ctgggagaag tgctcggtct gggccgcgcg gaggtggccg
     1081 cactgatgga tgcaggcgcg atcgaatgaa ggtccggcgc gtcgaggccg gagatcgcga
     1141 atgggtcgcg gaagtggtgc gcacgcactt cgcgtcgacg cgcatggtgt cgcgcggccg
     1201 gttgtacgaa gacgtctctg tgctcgacgg gttcatcgtc gaaggtgacg gccggccgat
     1261 cgggttgtcg ctgtgggagg agcgcgacgg cgatgcggag ctcgtcgtga tggtcacgct
     1321 caaccgcggc agcggcgcgg gcacgatgtt gatcgacgcc gtcgtcgagt acgcccgcga
     1381 gcacgactgg cagcgcttgt ggctgatcac gacgaacgac aacgtcgacg cgatccgatt
     1441 ctaccagcgc gcgggctggg actgggtcgg cttctaccgc gacgcggtga ccgaggcgcg
     1501 cgggttgaag ccggagatgg gcgagctcgg cgcgcacggg ctcccgatcc gccacgagat
     1561 cgagttcgaa gcgccgctct aggtcacacc gtcgccgcga cgacgacgta ctgcaacgcg
     1621 aactcgaagc caccggcttc acgcgctcgt tgcatcgcgt cgtcccagac gcgtacgtcg
     1681 gcatcagtga actcgccctc cgcccgccag tagtcgaccc acgtcggaag accgaacgcg
     1741 tcgtcggggt tcgtcagggc cagcgtgaac tcgtcgaccg tgagctcggc gtcgtcgcgc
     1801 tcgtcgaagg tgtcgacgac ctcccggatg aacgtgccgt tgcgataccc gacctgggcg
     1861 cgcaaacggg tcacggcatc gaccagcgcc gcaggcgcac ccgggacgac gatcgacagg
     1921 gtcgattggt cggggtcggc gaccacaatg cgaccactcg tgacccggac gagctcatcg
     1981 aacgcgcggt gcggatcggc gacgtgttgc acgacacggt cggcgcgcac cgcggcgaac
     2041 gtgcggtcgg caaacgggag cgcgtgcatg tcggcgcgcc cgaccgcgtg tccgcgttcg
     2101 tgcgaacgc
//
LOCUS       DASUGA010000315         3732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1277740, whole genome shotgun sequence.
ACCESSION   DASUGA010000315 DASUGA010000000
VERSION     DASUGA010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277740"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2108
                     /gene="ppdK"
                     /locus_tag="VFX21_06190"
     CDS             <1..2108
                     /gene="ppdK"
                     /locus_tag="VFX21_06190"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX5095582.1"
                     /translation="AGLVTTFKGIVKSEAGIDFPDDPTAQLAHAIEAVFKSWNGRRAK
                     DYRKLEGIADDLGTAVNVQTMVFGNKGDDSGTGVAFTRNPSTGEAAPYGDFLVNAQGE
                     DVVAGIRITEPLNEMGGHFPEPHQQLLGLMQLLEGHYHDMCDIEFTIEQGRLFMLQTR
                     VGKRTAAAALRMAVEMEQAGLIDKKEAVLRVQPAQLDQLLHPQFDPKAAYDVLAKGLN
                     ASPGAAVGKVYFTADDAEARHEAGERVILVRPETSPDDLHGMIAAEGILTSRGGLVSH
                     AAVVARGMGTPAICGAEALKIDLKAKQFVVDGTTVKEGDVISISGTTGEIVVGEVAVI
                     TPEPTGHFGTILGWADEFRRLKVRTNADLPEDAQKARDFGAQGIGLCRTEHMFLGDRL
                     PIVQRMILADTPDEEAAALEQLRAQQEEDFVGILEAMDGLPVTVRLLDPPLHEFLPDH
                     EELIVKQAKGEATDADLKLLHAAEYWRESNPMLGTRGCRLGIIKPGLYRMQVRALVQA
                     AVDRKKAGGDPIIEIMIPLIVSEPELKLIDDWCREEADAVIAAAGVELAYLVGTMVET
                     PRAALVADRIADVAEFFSFGTNDLTQMTFGFSRDDIEGRFMEAYLDYKLLKDNPFDSL
                     DIEGVGQLVKMGVEKGRTTRPDLKLGICGEHGGDPRSVAFCHEVGLDYVSCSPYRVPI
                     ARLAAAHAAIGSGGPGTTA"
     gene            2225..3304
                     /locus_tag="VFX21_06195"
     CDS             2225..3304
                     /locus_tag="VFX21_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002519039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5095583.1"
                     /translation="MQVDEELLRQAHPGASAFVGRAVLRRADREAALDATMAPGATRP
                     VGAGKRALAEEPDPFRLCFERDLDRVKHSAPWRRLAGKCQVFIAPEDDHLRTRLTHAV
                     EVAQIATGIARAAGLCVPLAEAIALAHDCGHGPAGHASEEAFSPYVPGGYDHAVYGAD
                     VTLAPLNLCAETLDGVRNHSWRRPAPSTPEGEVVAWADRIAYVCHDFEDAVRAGILVP
                     GDLPEEVAAVVGRSRSRQIGAFVYAVLDAIDRSGSVGMTEPGANALAAFRAFNFDRIY
                     LRPAARKQADKVIGLLQGLVDFFADAPSRIPADATGMHPGITPGSLEAAEIAVRYVSG
                     MTDRYALGLGVELLGWRPEELPRGV"
     gene            3323..>3732
                     /locus_tag="VFX21_06200"
     CDS             3323..>3732
                     /locus_tag="VFX21_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019553225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HEX5095584.1"
                     /translation="MGILDDDVARVREATDLVALISEHVALKRVGRRFQGLCPFHQEK
                     SPSFSVSPEKGVFHCLAGETRVITWEGVKEIRELAGETHRVLTERGSWVDAPFSAFGK
                     QRLYKISLTRNRQKKEIYATDGHRWFLRWGPRMT"
BASE COUNT          636 a         1219 c         1270 g          607 t
ORIGIN      
        1 tcgccggttt ggtcacgacg ttcaagggca tcgtgaagtc ggaagccggg atcgactttc
       61 ccgacgaccc gaccgcgcag ctcgcgcacg ccattgaagc ggtgttcaag tcgtggaacg
      121 gccgccgggc gaaggactac cgcaagctcg aaggcatcgc cgacgacctc ggcaccgccg
      181 tgaacgtgca gacgatggtg ttcggcaaca agggcgacga ctccggcacc ggcgttgcat
      241 tcacgcgcaa cccgtcgacc ggagaagcgg cgccgtacgg cgacttcctc gtcaacgcgc
      301 agggcgaaga cgtcgtggcc ggcattcgca tcaccgagcc cttgaacgag atgggcggtc
      361 acttccccga gccgcaccag cagctcctcg ggctcatgca gttgctcgaa ggtcactacc
      421 acgacatgtg tgacatcgag ttcacaatcg agcagggccg cctgttcatg ctgcagacgc
      481 gcgtcggcaa gcgcaccgcg gccgccgcgc tccgaatggc ggtcgagatg gaacaggccg
      541 ggctcatcga caagaaggaa gcggtgctgc gcgtgcagcc cgcgcagctc gaccagttgc
      601 tgcacccgca gttcgacccg aaggccgcat acgacgtgct cgccaagggc ttgaacgcgt
      661 cgcccggcgc cgcggtcggc aaggtgtact tcaccgccga cgacgccgaa gcgcgccacg
      721 aggcggggga gcgcgtcatt ctcgttcgcc ccgagacgtc gcccgacgac ttgcacggca
      781 tgatcgcggc cgagggcatc ctcacgtcgc gcggtggtct cgtgtcgcac gcggcggttg
      841 tggcgcgcgg catgggtacg cctgcgatct gtggcgccga agcgttgaag atcgatttga
      901 aggcgaagca gttcgtcgtc gacggcacga cggtgaagga aggcgacgtc atttcgatct
      961 ccggtacgac cggtgaaatc gtcgtcggcg aggttgccgt catcacgccc gaaccgaccg
     1021 ggcactttgg cacgatcctc ggttgggccg atgagttccg ccgcctcaag gtgcgcacca
     1081 acgccgacct gcccgaggat gcgcagaagg cgcgcgactt cggcgcgcag ggcatcggcc
     1141 tgtgccgcac cgagcacatg ttcctcggcg accggctccc gatcgtgcaa cgcatgatcc
     1201 tcgccgacac ccccgacgag gaagccgccg cgctcgagca gttgcgcgca cagcaggaag
     1261 aagacttcgt cggcattctc gaagcgatgg acggtctgcc ggtcacggtg cgactgctcg
     1321 acccgccgct ccacgagttc ctgcccgacc acgaagagct catcgtgaag caggccaagg
     1381 gcgaggcgac cgacgccgac ctcaagctgc tgcacgcggc tgagtactgg cgcgagtcca
     1441 acccgatgct gggcacccgc ggttgccggc tcggcatcat caagcccggc ctctaccgca
     1501 tgcaggttcg cgcactggtg caggccgcag tcgatcggaa gaaggcgggc ggcgacccga
     1561 tcattgagat catgatcccg ctgattgtga gcgagcccga actgaagctc atcgacgact
     1621 ggtgtcgcga agaggccgac gcggtgatcg cggcggccgg cgtcgagctt gcgtacctcg
     1681 tcggcacgat ggtggagacg ccgcgcgccg cgctcgtcgc cgatcgcatc gcggatgtcg
     1741 ccgagttctt ctcgttcggg acgaacgacc tcacgcagat gacgttcggt ttctcgcgtg
     1801 acgacatcga gggccgcttc atggaggcgt acctcgacta caagttgttg aaggacaacc
     1861 cgttcgactc gctcgacatc gaaggtgtcg gccagctcgt gaagatgggc gtcgagaagg
     1921 gacgcacgac gcgtcccgac ttgaagctcg gcatctgcgg cgagcacggc ggcgacccgc
     1981 gttcggtcgc gttctgtcac gaagtcggcc tcgactacgt gtcgtgctcg ccgtaccgcg
     2041 tgccgattgc ccgcctcgcc gccgcccacg ccgcaatcgg ctccggcggc cccggcacaa
     2101 ccgcgtagtt ttcggcgccg aaccagaggt tcggcaccga ggtcgagggg gaacgcaagc
     2161 ttgtccccct cgaggacccc ctgcgcacca cgtttctgtc tcaccctgtc gttacgctgt
     2221 tcgtatgcag gtggacgagg agctgctccg gcaggcgcat ccgggcgcgt cggcgttcgt
     2281 agggcgcgcg gtgctgcggc gcgccgaccg ggaggctgcg ctcgacgcga cgatggcgcc
     2341 cggtgcgacg cggcccgtcg gtgcgggcaa gcgcgcgttg gctgaagagc ccgatccgtt
     2401 ccggctgtgc ttcgaacgtg atctcgaccg cgtgaagcac tccgcgccgt ggcggcgact
     2461 cgcggggaag tgccaggtgt tcatcgcgcc ggaagacgac catttgcgca cgcggctcac
     2521 acatgcggtc gaggtggcgc agatcgcgac cggtatcgct cgcgcggccg gtttgtgtgt
     2581 accgctcgcg gaagcgatcg cactcgcgca cgattgcggg cacggtcccg cggggcatgc
     2641 gtcggaggaa gcgttctcgc cgtacgtgcc gggcggttac gaccatgccg tatacggcgc
     2701 cgacgtcacg ctcgcgccat tgaacttgtg tgcggaaacg ctcgacggcg tgcgcaacca
     2761 ctcgtggcgg cgcccggcac cgtcgacccc cgaaggcgaa gtggtcgcgt gggcggaccg
     2821 catcgcgtac gtgtgccacg acttcgaaga cgcagtgcga gcgggcatct tggtgcccgg
     2881 cgatctgcct gaggaggtgg ccgcggtggt tgggcgcagc cggtcccgtc agatcggagc
     2941 gttcgtctac gccgtgctgg atgccatcga ccgcagtggt tcagtcggga tgaccgagcc
     3001 cggtgcgaat gccttggcgg cgttccgggc gttcaacttc gaccggatct acctgcggcc
     3061 ggccgcgcgc aagcaggccg acaaggtgat cgggctgctg cagggcctcg tcgacttctt
     3121 cgccgatgca ccgagccgca ttcccgccga cgccaccggc atgcacccgg ggatcacgcc
     3181 gggctcgctc gaagcggccg aaatcgccgt ccgctatgtc agcggcatga ccgaccgtta
     3241 cgcattgggt ctcggggtcg aactcctcgg ctggcgcccc gaggaacttc cccgcggcgt
     3301 gtaaccccgt agtctgcggc caatggggat cttggacgac gacgtcgccc gagttcggga
     3361 agcaaccgat cttgtcgcgt tgatcagcga acacgtcgcg ctcaaacgcg tcggtcgtcg
     3421 cttccagggg ctgtgtccgt tccaccagga gaagtcgccc tcgttttcgg taagcccgga
     3481 gaagggtgtg ttccactgcc tcgcggggga gacacgagtc atcacgtggg aaggggtcaa
     3541 ggagattcga gagctcgccg gggaaaccca tcgagttctg actgaacgag gcagctgggt
     3601 agacgcaccg ttctcggcgt ttgggaaaca gcggctctac aagatcagtc tcacgcgcaa
     3661 tcggcagaag aaagagatct acgcgaccga cgggcatcgc tggttccttc gctggggacc
     3721 gcgaatgacg aa
//
LOCUS       DASUGA010000316         4837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_27189, whole genome shotgun sequence.
ACCESSION   DASUGA010000316 DASUGA010000000
VERSION     DASUGA010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27189"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2461
                     /locus_tag="VFX21_06205"
     CDS             <1..2461
                     /locus_tag="VFX21_06205"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX5095585.1"
                     /translation="TPTDIDSTNLTGATVTIAANFAPGEDVLSFTNQLGISASYVAGT
                     MTLTGTATVADYETALRSITYSNTSEDPSPAPRTLAFVVSDASNPSVVATRSLTVAAA
                     NDTPTATTTGGSASYTENASAVVLDAGFGAADVDNTNLAGASLQITGNFASGEDALAF
                     TNQSGISGSWNAGSGLLTLSGSASVADYQAAIRIITYVDTSDDPLTATRTVTITVTDG
                     TATSAPATRDVDVTAVNDAPAITPTGSSLSFTENAAATAVDGALTVGDLDDASLTAAT
                     VAISSGYWNGEDELAFTDQSGITGSWDATSGALTLTGSATVAQYQTALRTVTYVDTSD
                     DPHAVARTVTFTVTDSALTSSPVTRSIDVVPVNDLPYASLATGSVTYVEDDAPVAIDS
                     TATVTDPDDSTLPSATVAVTSGYHNGQDVLAVTGGGAGIAASWDSATGTLALHGAASP
                     AGYQSVLRSVVFSNTSQHPTSGARVLTVTLADASGPGGSVTRTVVVSAVDDAPVAVDD
                     SDTTFEAVGITVDVLENDTDIEDDQLSIAAVDVPAHATATVTSDQKLHVAPDADFAGS
                     MSVDYTVSDGSATDTGTLTLTVLRVADMGVDLTAAPNPVTVGDDILVDIGVGNAGPGI
                     AVHPVAYFDTGAGHRITSVRSDDGTCGAVGAQGRCDFVDIAAGDTVHVTVAVRTGGAG
                     TLTAGAIVTAEPLDFHVADNGDEAELIVRSRTRSSAPRSPATTTTVPQESSSTTSSTS
                     SASSATTTTTTTTHHASTTTTTRPVTPTTHADAPPTTIAETDEKSEGGRSGVGLYVGV
                     LVTIAAAIAAAVVLIQRRVGS"
     gene            2458..3291
                     /locus_tag="VFX21_06210"
     CDS             2458..3291
                     /locus_tag="VFX21_06210"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX5095586.1"
                     /translation="MIDLDVLARDAARLDPLPTSVARLASLIGAGDPPFDEIIELVRF
                     DQAMTASLLRTANSSWSASRSEVATVKDAVIRLGAGPVLSLAIAAHVQAQMTRAVPEL
                     GLGEHDLWDHSVAAMLAAEMLGRAATHRPPPETATAALLHDVGKLIMARHLDPPLLAA
                     LAASETAGHSRMQAERDVLGVTHAELGGLIAQSWGLPETLARGIRLHHEPDPRDGAIA
                     CAVHLADLIATAVAAGAGVDDAGLRFDFALGQLGLTPAAVEETGRLVAERLVEVQDRL
                     R"
     gene            3466..>4837
                     /locus_tag="VFX21_06215"
     CDS             3466..>4837
                     /locus_tag="VFX21_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX5095587.1"
                     /translation="MTAALPSGATAPDYDDVWATVQDGDSGEVSVVRGADAVVAAVDD
                     RLGRTDDNVLSIETDQPVASLGDPLRPQQWALNTTSYEATWSTTRGSGVTVAVIDTGV
                     RGDHEDLSSILLPGVDYVNGTDGRVDPNGHGTHVAGIIAAQVDNGRGIAGGAPGVNIL
                     PVRVLDANGSGSTSNVAAGIIWATDHGARVINLSLGGGESQGMHDAIIYAVNKGVVVL
                     AAAGNSGDGAAPVYPGAFNESIAVAAVDSNRNRASFSNTGSYVDIAAPGVSILSTYGS
                     AANAYAAMSGTSMATPYAAAAAALVLAAKPSLSSTGVRDLLLASADDLGSPGFDNWYG
                     NGFVDPRDAVNRALAPVPGTAGAGYWIVDRDGDVHAFGAAPYLGEPKGLPGLQPIVAS
                     ARTPSGGGYWIVAADGAVYAYGDAPALGGMNGRGLNQPIVAMAPTRTGRGYFLLARDG
                     GVFAFGD"
BASE COUNT          758 a         1803 c         1614 g          662 t
ORIGIN      
        1 cacgccgacc gacatcgaca gcacgaactt gaccggcgcg acggtgacga tcgccgccaa
       61 cttcgccccc ggtgaagacg tgctgtcgtt cacgaaccag ctcggcatct ccgcctccta
      121 tgtcgccggc acgatgacgc tcaccgggac ggcgaccgtc gccgactacg agaccgcgtt
      181 gcgctccatc acgtactcca acacgagcga ggatccgtcg cccgcgccac gcacactcgc
      241 cttcgtcgtc tccgacgcgt cgaacccgag tgtcgtcgcg acccgcagcc tcaccgtcgc
      301 cgcggccaac gacaccccga ctgcgaccac caccggcggc agcgcgtcgt acaccgagaa
      361 tgcatccgcg gtcgtgctcg acgccggctt cggtgcggcc gacgtcgaca acacgaacct
      421 cgcgggcgca tccctgcaga tcacgggcaa cttcgcgagc ggtgaagacg cgctcgcgtt
      481 caccaaccag tcggggatca gcggatcctg gaacgcgggc tcgggtctcc tgaccctcag
      541 tggcagcgcg tcggtcgcgg actaccaggc cgcgatccgc atcatcacct acgtcgacac
      601 gagcgacgat cctttgacgg ccacccgcac cgtcacgatc accgtgaccg acgggacggc
      661 cacgagtgca cctgcgacgc gcgacgtcga cgtgacggcc gtcaacgacg cgccggcgat
      721 cacgccgacg ggttcgtcgt tgtcgttcac cgagaacgcg gccgcgaccg cggtcgacgg
      781 ggccctcacc gttggcgacc tcgacgatgc gtcgctcacc gcggcgacgg tcgcgatctc
      841 gagtggctat tggaacggtg aagacgagct cgcgttcacc gaccagtccg gcatcaccgg
      901 atcgtgggat gcgacgtcgg gtgcgctcac actcaccggc tccgccaccg tcgcgcagta
      961 ccagaccgcg ttgcgcaccg tcacctacgt cgacacgagc gacgacccac acgcggtggc
     1021 gcgcacggtg acgttcaccg tgaccgacag cgcgctgacg agctcgcccg tgacccgcag
     1081 catcgacgtc gtgcccgtga acgacctgcc gtacgcgtcg ttggcgacgg gcagcgtgac
     1141 ctacgtcgag gacgacgcac cggtggcgat cgacagcacc gcaacggtga ccgatcccga
     1201 cgactcgacg ctgccgagcg cgaccgtcgc cgtcacgagc ggctaccaca acggtcagga
     1261 cgtcctcgcg gtgaccggcg gcggcgcagg tatcgccgca tcgtgggact cggcaaccgg
     1321 caccctggcc ttgcacgggg cggcgagccc cgccggctat cagtcggtgc tgcggtcggt
     1381 cgtgttctcc aacacgtcgc agcatccgac ctccggtgcg cgggtgctga ccgtcaccct
     1441 cgccgacgcg agcggccccg gtggcagcgt gacccgcacg gtcgtcgtca gcgccgtcga
     1501 cgacgcgccg gttgcggtcg acgacagcga tacgaccttc gaagccgtcg gtatcacggt
     1561 cgacgtgctc gagaacgaca ccgacatcga ggacgaccag ctctcgatcg ccgcggtcga
     1621 cgttccggcg cacgcgacgg cgacggtcac gtcggatcag aagctgcacg tcgctcccga
     1681 cgccgacttc gccgggagca tgtcggtcga ctacacggtc tccgacggca gtgcgacgga
     1741 caccggcacg ctcacgctca ccgtgctccg ggtcgcggac atgggcgtcg acctgaccgc
     1801 cgcgcccaac ccggtgacgg tcggcgacga catcctcgtc gacatcggcg tcggcaacgc
     1861 cggacccggg atcgccgtgc accctgtcgc ctacttcgac acgggcgccg gccaccgcat
     1921 cacatccgtg cgcagcgacg acggcacgtg cggcgcggtt ggcgcccaag gtcgctgtga
     1981 cttcgtcgac atcgccgccg gcgacaccgt gcatgtcacg gttgccgtcc gcaccggcgg
     2041 ggcgggcacg ctgaccgccg gggcgatcgt caccgccgaa ccactcgact tccatgtcgc
     2101 cgacaacggc gacgaagccg agctgatcgt gcgcagccga acgcgttcgt cggcgccgcg
     2161 gtcgccggcg accacgacga ccgtgccgca ggagtcgagc tcgacgacgt catcgacctc
     2221 gtccgcctcg tcggcgacca ccaccaccac caccaccacg caccacgcgt cgacgacgac
     2281 cacgacccgg cccgtgacgc ccaccacgca cgccgacgcg ccgccgacga cgatcgcgga
     2341 aacggacgag aagtccgagg gcggccgcag cggtgtcggc ctctacgtcg gtgtgctggt
     2401 cacgatcgcg gccgcgatcg cggccgctgt cgtgctcata caacgccggg tcggctcgtg
     2461 atcgatctcg acgtcctcgc ccgggatgcg gcgcggctcg acccgctgcc gacgagcgtc
     2521 gcgcgtcttg cgtcgttgat cggtgccggt gacccgccgt tcgacgagat catcgagctc
     2581 gtgcggttcg accaggcgat gacggcatcg ctgttgcgga ccgcgaactc gtcgtggagc
     2641 gcgagccgtt cggaagtcgc gacggtcaag gacgcggtca ttcgcctcgg tgccggaccg
     2701 gtgctctcgt tggcgatcgc cgcgcacgta caggcgcaaa tgactcgcgc ggtgccggag
     2761 ctcggcttgg gtgagcacga cctgtgggac cactcggtgg ccgcgatgct cgccgccgag
     2821 atgctggggc gcgccgcgac gcaccgaccg ccgcccgaga ccgcgaccgc ggcgctgttg
     2881 cacgacgtcg gcaagctgat catggcccgc catctcgacc cgcccctcct cgccgcgctc
     2941 gccgcgtcgg agaccgccgg tcacagccgg atgcaggccg agcgcgacgt cctgggcgtc
     3001 acccatgccg agctcggcgg cctcatcgcg cagtcatggg ggttgccgga gacgctcgca
     3061 cgcggcatcc gcctccacca cgaacccgac ccgcgcgacg gcgccatcgc gtgcgcagtg
     3121 cacctggccg acctgatcgc caccgcggtc gcggccggcg ccggtgtcga cgatgccggc
     3181 ctgcgcttcg acttcgccct cggtcagctc ggcctgaccc cggcggcagt cgaagagacg
     3241 gggcgtctgg tcgcagaacg cctggtcgag gtgcaggacc ggcttcggta gcgtccgcag
     3301 tcgtcgaatc tcacgctcag tgacaaaatt gcggttaggg tcgccactgc ctgtgccgat
     3361 tgccactcca tgcggttggg agacggcaac tcatcgggca agatcgggca ccggggccgg
     3421 aaggtcgcgc ggtacgtggt ggcagcgggt ctgagcatcg gcctggtgac cgcggcgctc
     3481 cccagcggcg ccaccgcccc cgactacgac gacgtctggg cgaccgtcca agacggcgac
     3541 agcggcgagg tctccgtcgt ccgcggcgcc gacgcggtgg tcgcggcggt ggacgaccgg
     3601 ctgggccgga ccgacgacaa cgtgctgagc atcgagaccg accagcccgt cgcgagcctc
     3661 ggcgacccat tgcggcccca gcaatgggcg ctcaacacca cgtcgtacga agcgacgtgg
     3721 tcgaccacgc gcggcagtgg ggtgacggtc gctgtcatcg acaccggcgt ccgcggtgac
     3781 cacgaggacc tgtcctccat cctgctgccg ggcgtggact acgtgaacgg caccgacggt
     3841 cgcgtcgacc ccaacggtca cggcacgcac gtggccggca tcatcgccgc ccaagtggac
     3901 aacgggcgcg ggatcgcagg cggcgcgccc ggtgtcaaca tccttccggt acgcgtgctc
     3961 gacgcgaacg gcagcggcag cacgtcgaac gtcgcggccg ggatcatctg ggccaccgac
     4021 cacggcgcgc gcgtcatcaa cttgagcctc ggcggcggcg agtcgcaagg catgcacgac
     4081 gcgatcatct acgcagtcaa caagggtgtc gtcgtgctcg cggccgcggg caacagcggc
     4141 gacggcgccg cacccgtgta ccccggtgca ttcaacgaat cgatcgcagt cgccgccgtc
     4201 gactcgaacc gcaaccgcgc atcgttctcc aacaccggca gctacgtcga catcgcggcg
     4261 cccggtgtca gcattctctc gacctacggc tcggccgcca acgcgtacgc ggcgatgagt
     4321 gggacgtcaa tggcgacgcc ctacgccgcc gccgccgcgg cactcgtgct ggcggccaag
     4381 ccgagcctct cgtcgaccgg cgtgcgcgac ctgttgctcg catccgcgga cgacctcggg
     4441 agtcccggct tcgacaactg gtacggcaac gggttcgtgg acccgcgtga cgcggtgaac
     4501 cgcgcgctcg ccccggtccc cggcaccgcc ggcgccggct actggatcgt cgaccgcgac
     4561 ggcgacgtgc acgcgttcgg cgccgcgccg tacctcggcg aaccgaaggg cctgcccggt
     4621 ctgcagccga tcgtcgcctc ggcccgcacg ccaagcggcg gcggctactg gatcgtcgcc
     4681 gccgacggcg cggtctacgc atacggcgac gcgccggcgc tcggcggcat gaacggtcgc
     4741 ggcctcaacc agccgatcgt cgccatggcg ccgacgcgca ccggacgggg ctacttcctg
     4801 ctcgcccggg acggcggtgt gttcgccttc ggtgacg
//
LOCUS       DASUGA010000317         2942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_924122, whole genome shotgun sequence.
ACCESSION   DASUGA010000317 DASUGA010000000
VERSION     DASUGA010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924122"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VFX21_06220"
     CDS             <1..445
                     /locus_tag="VFX21_06220"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095588.1"
                     /translation="VLREHVPGLHVTTSGTHVIEGMPMSWRTRDAIESLGLPIPNHRS
                     RQATVQELDSASLVIALAREHVAWMRRVHPRAAPRTATLKRLARDLPPGPAPLPERLG
                     AMALDEVELESWEDVLDPAGGDLDVFLECAREIADLLHVVIPRLD"
     gene            447..2126
                     /locus_tag="VFX21_06225"
     CDS             447..2126
                     /locus_tag="VFX21_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX5095589.1"
                     /translation="MAIGVPEDLARIEAELLASGGYFAIETTEVNGASVQAFASRARS
                     LGELLEGSLNRADAPYFTFTDGAHERSLTFGEHAAVVGSVAAALRDQYGVQPGDRVAI
                     LAANTAEWIVTFWATVSLGAIAVGLNGWSTATEIEYFLGDSEPRLLVADAKRLARLDR
                     RAVAAPVVEMEREFGALEEYAPGAALPDVPIAEDDPAIILYTSGTTGRPKGAVNTHRN
                     MIAFVSMNFFGGARTMMLHPPPPGMGPRCQLMTNPLFHVSGLHAGAVMLLASGVRSVW
                     LTGRFDAEVAMQVIERERCTGWSYTETMLHRFLNHPALPNYDLTSVMSVGGGGSPIAP
                     SIFERTRQVFPNAAPTMGYGYGQTECAALATMNLGQELVDFPLSVGRPLPTVELEIRS
                     PNDTPLAEGEEGEVCVKGPMVMPGYWRRPEENANTIRDGWLHTGDIGRMEGGRLYLSS
                     RKRDLIFRGGENVYPVEIEKRIEDHPAVEECAVIGVDDPDLGQAVKAVVVAAPGATID
                     VESLRAWTADALAYYKVPAHWEVRDALPRNAAGKILKDALRTGEDAAFVEE"
     gene            2137..2760
                     /locus_tag="VFX21_06230"
     CDS             2137..2760
                     /locus_tag="VFX21_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX5095590.1"
                     /translation="MAIDLHALVAPGHTALVLQEVQEGVVGAESALPALASAAAAVGL
                     IENCARLAAAARAAGIPVVHCTAETRDDGRGANQNARLFLGVAKSPVRLSPGSRAVQV
                     PDAIGTPPEDIVLPRYHGLGPMTGTQLDSMLRNLGTRTIVGVGVSVNIGMTNFAFDAV
                     NRGYQMVMPRDAVAGVPPEYAEAVLDNTLSLVATLTTTEAVLQAWGS"
     gene            complement(2720..>2942)
                     /locus_tag="VFX21_06235"
     CDS             complement(2720..>2942)
                     /locus_tag="VFX21_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095591.1"
                     /translation="GLAWRGNEGSLAEPLAGVVPRGYTAHITFLGSGRCSLRSIFARV
                     AYLRDARADGASPAPLLKIPTPATQPRSS"
BASE COUNT          457 a         1005 c         1046 g          434 t
ORIGIN      
        1 ggtcctgcgc gagcacgtgc ccggtttgca cgtcacgacg tcgggcaccc acgtcatcga
       61 aggcatgccg atgagctggc gcactcgcga cgcgatcgag tcgctgggcc tgccgatccc
      121 gaaccaccgc agccggcagg caacggtgca ggagctcgac agtgcctcgc tggtcatcgc
      181 gctcgcacgc gagcacgtgg catggatgcg ccgcgtgcat ccccgcgccg cgccgcgcac
      241 cgcgacgctc aagcgactgg cgcgtgacct gccgccgggc cccgcgccgt tgcccgaacg
      301 gttgggagcg atggcgttgg acgaggtcga gctggagtcg tgggaagacg tgctcgatcc
      361 ggccggcggt gatctcgacg tgttcctcga gtgcgcgcgc gagatcgcgg acctgttgca
      421 cgtcgtgatc ccgagactcg actgagatgg cgatcggcgt tcccgaggac ctggctcgta
      481 tcgaggcgga actgctcgcg tccggtggct acttcgcgat cgagacgacg gaggtcaacg
      541 gcgcgtcggt gcaggccttc gcgagtcgcg cccgcagtct gggcgagctg ctcgaaggat
      601 cgctcaaccg cgccgacgcg ccgtacttca cgttcaccga cggcgcgcac gaacggagcc
      661 tgaccttcgg ggagcacgcg gcggtcgtcg gatcggtcgc ggctgcgctg cgcgaccagt
      721 acggcgtgca acccggtgat cgcgtcgcga tcctcgcggc caacaccgcc gagtggatcg
      781 tcacgttctg ggcaacggtg agcctcggcg cgatcgcggt cgggctcaac ggctggtcga
      841 cggccacgga gatcgaatac ttcctcggag acagcgagcc gcggctgctc gttgccgacg
      901 ccaagcggct ggcgcgcctc gaccggcgcg cggttgcagc accggtcgtc gagatggaac
      961 gcgagttcgg ggcgctcgag gaatacgcgc ccggggcggc gctgcccgat gtgccgatcg
     1021 cggaggacga ccccgcgatc atcctctaca cgtcgggcac gacgggacga ccgaagggcg
     1081 cggtcaacac gcaccgcaac atgatcgcgt tcgtctcgat gaacttcttc ggtggcgcgc
     1141 gcacgatgat gctccacccg ccgccgcccg gcatgggtcc gcggtgccag ctcatgacga
     1201 acccgttgtt ccacgtctcg ggactgcacg cgggcgcggt catgctgctc gcgtccggtg
     1261 tgcgcagcgt gtggctcacc ggccgcttcg acgccgaggt cgcgatgcag gtcatcgagc
     1321 gcgagcgctg cacgggctgg tcgtacaccg agaccatgct gcaccggttc ctcaaccatc
     1381 ccgcgctccc gaactacgac ctcacctccg tcatgtccgt cggtggtggc ggttcaccga
     1441 tcgcgccgtc gatcttcgag cgcacgcgcc aggtgttccc gaacgcggcg ccgacgatgg
     1501 gctacggcta cggacagacc gagtgcgccg cgctcgcgac gatgaatctc ggtcaggagt
     1561 tggtcgactt cccgttgtcc gtcggccgcc cgttgccgac ggtcgagctg gagatccgta
     1621 gcccgaacga cacgccgctc gcagagggtg aggagggcga agtctgcgtc aagggcccga
     1681 tggtgatgcc gggctactgg cgacgaccgg aagagaacgc gaacacgatt cgcgacggat
     1741 ggctgcacac cggcgacatc gggcgcatgg aaggcggccg cctgtacttg tcgtcgcgca
     1801 aacgcgatct catcttccgc ggcggcgaga acgtgtaccc ggtcgagatc gagaagcgca
     1861 tcgaagacca cccggccgtc gaggagtgcg cggtgatcgg tgtggacgat cccgacctcg
     1921 gtcaggcggt gaaggccgtc gtcgtcgccg cgccgggcgc gacgatcgac gtcgagtcgc
     1981 tgcgggcgtg gaccgccgat gcgctcgcgt actacaaggt gcccgcgcac tgggaggtgc
     2041 gcgacgcctt gccgcgcaac gccgccggca agatcctgaa ggacgcactg cgaaccggcg
     2101 aagacgccgc gttcgtcgag gagtgaggag gccgacgtgg cgatcgacct gcacgcgctc
     2161 gtcgcgcccg ggcacaccgc cctggtgctc caagaagtgc aagaaggtgt cgtcggcgcc
     2221 gaatccgcgc tgcccgcgct cgcgtcggcc gcggctgcgg tcggtctgat cgagaactgc
     2281 gcccgcctgg cggccgcggc ccgcgccgcc ggcattcccg tcgtgcactg cacggccgag
     2341 acgcgcgacg acggccgcgg cgccaatcag aacgcgcgcc tcttcctcgg cgttgccaag
     2401 tcgccggtac ggctctcgcc cggcagccgt gcggtgcagg tgcccgacgc gatcgggaca
     2461 ccacccgaag acatcgtgtt gccgcgttac cacgggctcg gacccatgac cggcacacaa
     2521 ctcgactcga tgttgcgcaa cctcgggacg cgcacgatcg tcggtgtcgg cgtgtcggtc
     2581 aacatcggga tgacgaactt cgcgttcgac gcggtgaacc gcggttacca gatggtgatg
     2641 ccgcgcgatg ccgtcgcggg cgtaccgccg gaatatgccg aggcggtgct cgacaacacg
     2701 ctctcgctgg tcgcgacgct tacgacgacc gaggctgtgt tgcaggcgtg gggatcttga
     2761 ggagcggcgc aggcgacgcg ccgtccgcgc gggcgtcgcg caggtaggcg acgcgcgcaa
     2821 agatcgagcg cagcgagcag cgccccgacc cgaggaacgt gatgtgcgcg gtgtacccgc
     2881 gcggcacgac gcccgcgagc ggttcggcca ggctgccctc gttgccccgc cacgcgagcc
     2941 cg
//
LOCUS       DASUGA010000318         2437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1024945, whole genome shotgun sequence.
ACCESSION   DASUGA010000318 DASUGA010000000
VERSION     DASUGA010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024945"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1511)
                     /locus_tag="VFX21_06240"
     CDS             complement(<1..1511)
                     /locus_tag="VFX21_06240"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018539811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX5095592.1"
                     /translation="MSRTTKLPTLPRRHAKSSTPDSTIRPLLETLRKRPGRTDTAMIE
                     RAYLVARDAHRDQVRRSGDSYITHPVGVALILADLGLDDVTLAGALLHDAVEDTSVTL
                     DDLEREFGSDVAAIVDGVTKLDRLQFDSREAQQAATLRKMLVAMAKDVRVLLIKLADR
                     LHNMRTIASLPEAKQKRIAQETLDIYAPLAHRLGIQDVKWQLEDLAFAVLHPKRYAEI
                     EAMVLQHAGEREKYLHEVLDAVRARLDDLHIKAEVTGRPKHYWSIYEKMVVRGKEFAE
                     INDLVGVRVIVGSVKDCYGALGSIHALWRPVQGRFKDYIAMPKFNLYQSLHTTVVGPQ
                     GRAVEFQIRTSEMHNRAEYGVAAHWGYKEKQNPTEDLVWLQRIVDWQTETDDPADFMQ
                     SLKIDLEQDEVFVFTPKGKVVTLPTGATPVDFAYTIHTDVGHRCIGARVNGRLVPLDS
                     ILQSGDTCEIFTSKVEGAGPSRDWLQFVSTPRARSKIRQWFSRERRVDAIDQGR"
     gene            complement(1504..2028)
                     /locus_tag="VFX21_06245"
     CDS             complement(1504..2028)
                     /locus_tag="VFX21_06245"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012163376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX5095593.1"
                     /translation="MDPAWLKSHVRDIPDYPKPGIIFRDITPLLAAPDAFAATVDALA
                     APFEGERIDKVLGIEARGFVFAAPVAYHFAAGFVPVRKAGKLPWQIEQEEYELEYGTD
                     LLEIHRDAVHPGERVLIVDDVIATGGTAAATARLVERLGGEVAGFSFALELVFLAGRA
                     KLPGRRVEAVVSYE"
     gene            complement(2066..>2437)
                     /locus_tag="VFX21_06250"
     CDS             complement(2066..>2437)
                     /locus_tag="VFX21_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018087631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX5095594.1"
                     /translation="VALLPVLSLLIVGSLILGATALEDFALALAVGLFIGAYSSIYVA
                     APLLAWWKSKEPQYRALAERRKRTTIAATAVATGVPVIPTEDYGGDPEPAPVAGVPGP
                     PAVPRTNIQARPRQQRGRKRK"
BASE COUNT          384 a          816 c          820 g          417 t
ORIGIN      
        1 cggccctggt cgatcgcgtc gacgcgacgt tcacgagaga accactggcg gatcttcgag
       61 cgagcgcggg gcgtcgacac aaactgcaac cagtcgcgcg acggcccggc gccttcaacc
      121 ttgctcgtga agatctcgca cgtgtcgccg gactgcaaaa tcgaatcgag cggtaccagc
      181 cggccgttca cgcgcgcgcc gatgcaacgg tgaccgacgt cggtgtggat cgtgtacgcg
      241 aagtcgaccg gcgtcgcgcc ggttggcagc gtgacgacct tgcccttggg cgtgaagacg
      301 aacacctcgt cctgttcgag gtcgatcttc aggctttgca tgaagtcggc ggggtcgtcg
      361 gtctccgttt gccagtcgac gatccgctgc aaccacacga ggtcttcggt cgggttctgc
      421 ttttccttgt agccccagtg tgccgccacg ccgtactcgg cccggttgtg catctccgac
      481 gttcggatct ggaactcgac cgcccggccc tgcggtccga ccaccgtcgt atgcaacgac
      541 tggtagaggt tgaacttcgg catggcgatg tagtccttga agcgaccctg caccggacgc
      601 cacaaggcgt gaatcgagcc gagcgcgccg taacagtcct tcaccgaccc cacgatcacg
      661 cgcacgccga ccaggtcgtt gatctccgcg aactccttgc cgcgcacgac catcttctcg
      721 tagatcgacc agtagtgctt cggccggccg gtgacttcgg ccttgatgtg caggtcatcg
      781 agacgagcgc gcactgcatc gagcacttcg tgcaagtact tctcgcgctc ccccgcgtgc
      841 tgcaggacca tcgcctcgat ttcggcgtat cgcttcgggt gcaacaccgc gaaggccagg
      901 tcttcgagtt gccacttgac gtcctggatt ccgagccggt gcgcaagcgg cgcgtagatg
      961 tcgagcgttt cctgcgcgat gcgcttctgc ttcgcctccg gcagcgatgc gatcgttcgc
     1021 atgttgtgca accggtcggc gagcttgatg agcaggacgc gcacgtcctt ggccatcgcg
     1081 acgagcatct tgcgcagcgt cgccgcctgc tgcgcttctc tcgagtcgaa ctggagccgg
     1141 tcgagtttgg tgacgccgtc gacgatcgcc gcgacatcgg acccgaactc gcgctccagg
     1201 tcgtcgaggg tgaccgacgt gtcttcgact gcgtcgtgca gcagcgcgcc ggcgagtgtg
     1261 acgtcgtcga gtccgaggtc agcgagaatc aaggcaacgc ctaccgggtg tgtgatgtag
     1321 gagtcgcccg accggcgtac ctggtcgcgg tgggcgtcgc gcgcgacgag gtaggcgcgc
     1381 tcgatcatcg cggtgtcggt tcggcccggg cgcttgcgga gcgtctcgag cagcggccgg
     1441 atcgtcgagt cgggcgtcga cgacttcgcg tgccggcgcg gcaacgtcgg aagcttggtg
     1501 gtcctactca tacgagacca ccgcttcgac gcgacggccg ggcagcttgg cgcggcccgc
     1561 gaggaacacc agctcgagcg cgaacgagaa cccggccacc tccccgccga gccgttcgac
     1621 gagacgcgcg gttgcggcgg cggtaccgcc ggttgcgatc acgtcgtcga cgatcaacac
     1681 ccgctcgccg gggtgcacgg cgtcgcggtg gatctccagc agatccgtgc cgtactcgag
     1741 ctcgtactcc tcttgctcga tctgccacgg caacttgccg gccttgcgga ccggcacgaa
     1801 ccccgccgcg aagtgatacg cgaccggcgc tgcgaaaacg aagccgcgcg cctcgatgcc
     1861 gagcaccttg tcgatgcgtt cgccctcgaa cggcgccgcc agcgcgtcga cggtcgcggc
     1921 gaacgcatcc ggggcggcca ggagcggggt gatgtcgcgg aagatgatcc cgggcttcgg
     1981 gtagtcgggg atgtcgcgca cgtgcgactt gagccacgca ggatccatcg cccaccaggc
     2041 taccggcggg cccgaacgag actgtttact tgcgcttgcg gccgcgctgc tggcgcggcc
     2101 gagcctggat gttggtcctc gggaccgccg gcgggccggg cacgccggcg accggcgcgg
     2161 gctcggggtc gccgccgtag tcctcggtcg ggatgaccgg cacgccggtc gcgaccgcgg
     2221 tggccgcgat cgtcgtgcgc ttgcgccgtt cggccaacgc ccggtactgc ggctccttgc
     2281 tcttccacca agcgaggagc ggcgcggcaa cgtagatcga cgagtacgcg ccgatgaaca
     2341 ggccgaccgc gagtgcgagc gcgaagtctt cgagcgcggt cgcgccgaga atcaaactgc
     2401 cgacgatcag cagcgacaac accggcaaca gcgcgac
//
LOCUS       DASUGA010000319         5106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1670109, whole genome shotgun sequence.
ACCESSION   DASUGA010000319 DASUGA010000000
VERSION     DASUGA010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1670109"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VFX21_06255"
     CDS             <1..620
                     /locus_tag="VFX21_06255"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5095595.1"
                     /translation="RTAGADRERSMSDDFDALRRLRPDRVQPDDPAEPTVFAREKERL
                     MTTISDNAPTPGRVMRMPDIYARLAYEDEYAAVAYLERVFQLAEIREARTEHGDTMLA
                     WLRTGDGVVMIGHANAEVHRIHSPHTIGNTTVQMMVYVHDVDAHYAHALAEGAQITMA
                     LEDAFYGERRYEATDLEGHRWHFAERFEDIKARGGTVADDPRGGK"
     gene            745..969
                     /locus_tag="VFX21_06260"
     CDS             745..969
                     /locus_tag="VFX21_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095596.1"
                     /translation="MQWQPRFRAGAVFGGALAGLRRSRTEERPTPNPGNELDELIAYG
                     HAALAARGAAPARIAEPEKASKRRGRRKKG"
     gene            1001..1071
                     /locus_tag="VFX21_06265"
     tRNA            1001..1071
                     /locus_tag="VFX21_06265"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1033..1035,aa:Gly,seq:tcc)
     gene            complement(1073..1492)
                     /locus_tag="VFX21_06270"
     CDS             complement(1073..1492)
                     /locus_tag="VFX21_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5095597.1"
                     /translation="MARAIDLPPEAQRRIANDQVVWLTTITDSGAPAPNPVWFVPDGE
                     SLVVYAAPESHKVHNIGRRNHVSLHFNSDPNGTDVVVINGDAELENDQRPSALPAYVK
                     KYERSVAEDLQQTFDDLDRQFHTRIRIRPTRVRLTEA"
     gene            complement(1535..2503)
                     /locus_tag="VFX21_06275"
     CDS             complement(1535..2503)
                     /locus_tag="VFX21_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5095598.1"
                     /translation="MIDFSGQAVIVTGAGRGLGRLYALDFARRGASVVVNDLGSTMGG
                     DGADTQVADAVVAEIQTAGGVAVASYESVATVAGGQAIVDGALDAFGRLDAVVSNAGI
                     FDTQPFEDLTPDAWRRMLEVHLDGAFHISQPAYRAMKSRGYGRFVFTASSAGLFGQPS
                     SAHYAAAKAGTFGLANVIAIEGATHGILANCVLPFGYSRMVSETVGDRDEVEPDPGFL
                     HAIEPELVVPMVVYLASRACELTHHSFSACAGRYARVFVGLGDGWVAATDGTPTAEDV
                     AAHWPGVVRAEPYTIPDSIFDEVFKVCAQLGIDQNRTGEKRARNQS"
     gene            2636..4003
                     /locus_tag="VFX21_06280"
     CDS             2636..4003
                     /locus_tag="VFX21_06280"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5095599.1"
                     /translation="MSRATRPLIAAMLAMTLVAAACSGDDDDTSSADAATAASSGPDA
                     TTAAASAVDYAAIGLWDDGPCNKDLAPLKIGLTTLFESPVLSLGDHAKALEASADAFN
                     ARGGANGACIDVTTCDDGGNLDQAVGCVRTFDDAGVVATVNDQTTAGLAEVSAAMIDA
                     GISRIGSNVAPEDWAAPNMYPLDASGTGSVFVLPQALIDNGVEKIGVLRPDLADASAL
                     IGLLRGVYEGKAELPADIVVTAGTTDYSQFVLGAAQQGADGVELALGEQEAVQVVRAA
                     EQLDSDLTIGATLAAFPHAQIAELGDMAETMVFLWSYPPGTAELPVYDAMRADLAASG
                     EEELQPENLSATALRSWIGLYGLLKIIRDAGTTDFSRASISALLNNAKDVPMLGIFGD
                     ENWTPALDHPGAFKRAGMNTWGVYKWDPEAEAPGDLQGNFVQTSTLDFDEILCGSPFG
                     APKPC"
     gene            complement(4028..4786)
                     /locus_tag="VFX21_06285"
     CDS             complement(4028..4786)
                     /locus_tag="VFX21_06285"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5095600.1"
                     /translation="MAVTVDLTGRRVLVTGASSGIGQAICRSVVAAGGAVAMLARREE
                     RLTELQSELGERAFGVPCDVTDLDALAAAVDESARGLDGLDGIVAAAGRGMVGTMSTG
                     DPERWRELMHLNVVAPLATARAAVGHFGASGRRDVVLIGSTGALTPMPGLGIYAATKR
                     GLRAAFDALRLELAPAGINVSYVMPGMFDTEGLMAPDVVDGDVPDFDTPMFVAGAGPA
                     STEPLSDVVAMMLGLPDGVAINEIVIRPTGQLRP"
     gene            4833..>5106
                     /locus_tag="VFX21_06290"
     CDS             4833..>5106
                     /locus_tag="VFX21_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5095601.1"
                     /translation="MTERDESYGAGLAELMNPDVARSPQPIYAMLHATPVFRLDGVGV
                     IVTTREAVDEVLRNPETFLSGGAHDLKTKRPLIPLQTDPPDHRRYRK"
BASE COUNT          856 a         1774 c         1736 g          740 t
ORIGIN      
        1 cgcgaactgc gggcgccgat cgggagagaa gtatgagcga cgacttcgac gcgctgcgcc
       61 ggctgcgccc ggaccgggtc cagcccgacg accccgccga accgacggtc ttcgcccgcg
      121 agaaggaacg actcatgacg acgatcagcg acaacgcgcc cacgcccgga cgcgtgatgc
      181 gcatgcccga catctacgcc cggctcgcgt acgaagacga gtacgccgcc gtcgcgtatc
      241 tggagcgcgt gttccagctc gccgagatcc gcgaggcgcg caccgagcac ggggacacga
      301 tgctcgcgtg gctgcgcacc ggcgacgggg tcgtgatgat cggtcatgcg aacgcggaag
      361 tgcaccgcat ccacagcccg cacacgatcg ggaacacgac ggtgcagatg atggtgtacg
      421 tgcacgacgt cgatgcccac tacgcgcacg cgctggccga gggggcgcag atcaccatgg
      481 cgctcgagga cgccttctac ggcgaacgcc gctatgaggc caccgacctc gagggccacc
      541 gatggcactt cgccgagcgc ttcgaggaca tcaaggcccg gggtggcacc gtcgccgacg
      601 acccccgggg cgggaagtag cggccccacc ggcacccgcc cggctcgtcc cggttcgcgg
      661 agaaccactg ttcgtcaccg tcgcgcgcgg gcgagaggac gagacgctgt tggcgacggg
      721 gcgagcgcgc acaagcggac gcgggtgcag tggcagccgc gctttcgcgc gggcgccgtg
      781 ttcggcggtg cgctggccgg gctgcgccga tcacgcacgg aggaacggcc cacaccgaac
      841 ccgggcaacg agctcgacga gctcatcgcg tacggtcacg ccgcgctcgc ggcgcgcggc
      901 gccgcgcctg ctcgcatcgc ggaaccggag aaggcgagca agcggcgcgg ccgtcgcaag
      961 aagggctgac ccgccgcgcg ctagcgtgga gccgtacgcc gcgggtgtag ctcaatggca
     1021 gagcttcaga cttccaatct gactacgcga gttcgattct cgtcacccgc tctcacgcct
     1081 cggtcaagcg gacgcgcgtc ggccgtatgc ggatgcgcgt gtggaactgc ctgtcgaggt
     1141 cgtcgaacgt ctgctgcagg tcttcggcaa cggagcgttc gtacttcttc acgtatgcgg
     1201 gcagcgcact cggccgctgg tcgttctcga gctcggcgtc gccgttgatg acgacaacgt
     1261 cagtgccatt cgggtcgctg ttgaaatgca gcgacacgtg gttgcggcga ccgatgttgt
     1321 gcaccttgtg actctccggc gcggcatata cgacgagaga ctcaccatcc ggtacgaacc
     1381 acacagggtt gggtgcgggc gcaccgctgt cggtgatcgt cgtcagccag acaacctggt
     1441 cgttcgcgat ccggcgctgc gcctcgggtg gaagatcgat cgcacgtgcc atgcgggcac
     1501 ctccagtggc ggtctcgagt cgatgtacct gcatctacga ctggttccgc gcccgttttt
     1561 caccggtacg attctgatcg ataccgagtt gcgcgcagac cttgaacacc tcgtcgaaga
     1621 tcgaatccgg gatcgtgtac ggctcggcgc gaacaacccc gggccagtgc gccgcgacgt
     1681 cctcggcggt aggtgtcccg tcggtcgcgg cgacccaacc gtcgcccaac ccgacgaaca
     1741 cgcgcgcgta acgaccggcg caggcggaga agctgtggtg cgtcagctca cacgcacgac
     1801 tcgcgaggta caccaccatc gggacgacca gctcgggctc gatcgcgtgc aggaagccgg
     1861 gatcgggttc gacctcgtcg cggtcgccga cagtttcgga caccatgcgc gagtacccga
     1921 agggaagcac acagttcgca aggatcccgt gtgtcgcgcc ttcgatcgcg atgacgttgg
     1981 cgaggccgaa cgtgcccgcc ttcgccgccg catagtgcgc cgagctcggc tggccgaaca
     2041 gtcccgccga tgacgccgtg aacacgaacc ggccgtaacc gcgcgacttc atcgcgcgat
     2101 acgccggttg gctgatgtgg aacgcgccgt cgaggtgcac ctcgagcatc cgccgccacg
     2161 cgtcgggcgt gaggtcctcg aacggctgcg tgtcgaagat gccggcgttc gacacgaccg
     2221 cgtcgagccg gccgaacgcg tcgagggcgc cgtcgacgat cgcttgaccg cccgcgacag
     2281 tcgcaaccga ttcgtacgac gcaaccgcga cgccgcccgc ggtctggatc tccgcaacca
     2341 ccgcgtcggc cacctgcgtg tcggcgccgt cgccgcccat ggtggatccg aggtcgttga
     2401 ccacgacgga cgcgccgcgc cgggcgaagt cgagcgcgta cagacgcccg agtccacggc
     2461 cggcacccgt cacgatcacc gcctgccccg agaagtcgat cacggcgcgg acggtacctg
     2521 accaaacagt tgttccaacg gagggatcgg ggtacggtcc tccggcgacg agcggcaggt
     2581 cgttcgcggt gctgcgtcgt ccggtttggt cgggtggtcc gaagggggtc gtgatatgtc
     2641 gcgcgccacc cgtccgctga tcgcggcgat gctcgcgatg acactcgtcg cggccgcgtg
     2701 cagcggcgac gacgacgaca cgtcgagcgc cgacgccgcg accgcggcgt cgtccggtcc
     2761 cgacgcgacg acggccgcgg caagcgcggt cgactatgcg gccatcggcc tgtgggacga
     2821 cggcccgtgc aacaaggatc tggcgccact gaagatcggt ctgacgaccc tgttcgagtc
     2881 gcccgtgctg tcgctcggtg atcacgccaa ggcattggaa gcgtccgccg acgcgttcaa
     2941 cgcgcgtggc ggcgccaacg gagcctgcat cgacgtcacg acatgtgacg acggcggcaa
     3001 cctcgaccag gcggtcggtt gcgtgcgcac gttcgatgat gccggtgtcg tggcgacggt
     3061 caacgaccag accacggccg gcctcgccga ggtctcggcc gccatgatcg acgccggcat
     3121 ctcgcgcatc ggctccaacg tcgcaccaga agactgggcc gcgcccaaca tgtacccgct
     3181 cgacgcgtcg ggcacgggct cggtgttcgt gctgccgcag gcgctgatcg acaacggcgt
     3241 cgagaagatc ggtgtgctcc ggcccgacct cgccgacgcg tccgcgctga tcggcctgct
     3301 gcgtggtgtc tacgaaggca aggccgagct gccggccgac atcgtcgtga ccgcgggcac
     3361 gaccgactac agccagttcg tgctcggcgc cgcgcaacaa ggtgccgacg gcgtcgagct
     3421 cgcgctcggc gagcaagagg cggtacaagt cgtccgcgcc gcggaacagc tcgactccga
     3481 tctgaccatc ggtgcgacgc tcgccgcgtt cccccacgca cagatcgccg agctcggcga
     3541 catggccgag acgatggtct tcctgtggtc gtatccgccg ggcaccgccg aactgccggt
     3601 gtacgacgcg atgcgcgccg atctcgcggc ctccggcgag gaagagctgc aacccgagaa
     3661 cctcagcgcg accgcgttgc ggagttggat cggcctgtac gggctcctga agatcatccg
     3721 cgacgcgggg acgaccgatt tctcgcgcgc atcgatctcg gcgttgttga acaacgcgaa
     3781 ggacgtcccg atgctcggca tcttcggtga cgagaactgg acgccggcgc tcgatcaccc
     3841 cggcgcgttc aaacgcgcgg gcatgaacac gtggggcgtc tacaagtggg atcccgaggc
     3901 cgaggcgccc ggcgacctgc agggcaactt cgtccagacg agcacactcg acttcgacga
     3961 gatcctctgc gggtcgccgt tcggtgcgcc caagccctgc tgagccggac cgagccggac
     4021 cggggcacta cggccggagc tgtcccgtcg gccggatgac gatctcgttg atcgcaacac
     4081 cgtccggcag gccgagcatc atcgcgacga cgtccgagag cggctcggtg gatgcggggc
     4141 ccgcgccggc gacgaacatc ggcgtgtcga agtcggggac gtcgccgtcg acgacatcgg
     4201 gcgccatcag tccttcggtg tcgaacatgc cgggcatcac atagctcacg ttgatgcccg
     4261 cgggtgcgag ctcgagccgc agcgcgtcga atgcggcccg caacccgcgt ttggtcgccg
     4321 catagatccc gagcccgggc atcggtgtca gcgcgcccgt cgaaccgatg agcaccacat
     4381 cgcgccgccc gctcgcgccg aagtgaccaa ctgcggcccg cgcggttgcg aggggcgcga
     4441 cgacgttgag atgcatcagc tcgcgccaac gctcggggtc accggtcgac atcgtcccga
     4501 ccatgccgcg gcccgcggcg gcgacgatgc cgtcgagacc gtcgagacca cgcgccgact
     4561 cgtcgactgc cgccgcgagc gcgtcgagat cggtgacgtc gcacggcacg ccgaacgcgc
     4621 gctcgcccag ctcgctctgc agctcggtga gccgctcctc gcggcgcgcc aacatcgcga
     4681 cggcaccgcc ggccgccacg acggatcggc agatcgcctg gccgatgccg ctggacgcgc
     4741 ccgtcaccaa cacgcggcgt ccggtgagat cgacggtgac agccatgggt gcattcttgc
     4801 aacgaaccaa acattgggtc cacactgcgc ccatgaccga acgggacgag agctacggcg
     4861 cggggctggc cgagttgatg aaccccgatg tggcgcgcag cccgcagccc atctacgcga
     4921 tgttgcacgc aacgcccgtg ttccgcctcg acggcgtcgg cgtcatcgtc acgacgcgcg
     4981 aagcggtcga cgaggtgctg cgcaatcccg agacgttctt gtcgggtggc gcgcacgatc
     5041 tcaagacgaa gcggccgctc atcccgctgc agacggatcc gcccgatcac cgtcgctacc
     5101 gcaagc
//
LOCUS       DASUGA010000320         3769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_533577, whole genome shotgun sequence.
ACCESSION   DASUGA010000320 DASUGA010000000
VERSION     DASUGA010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533577"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..184)
                     /locus_tag="VFX21_06295"
     tRNA            complement(111..184)
                     /locus_tag="VFX21_06295"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(148..150),aa:Ala,seq:tgc)
     gene            356..1462
                     /locus_tag="VFX21_06300"
     CDS             356..1462
                     /locus_tag="VFX21_06300"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX5095602.1"
                     /translation="MSAALVVRDLDAAIDKAFACEGALDHVEVARLADRLECLKLRAF
                     RDYARSEQWKEEGFVTAAAAVRAQCRLTEGAARHALDLGRKLEELPVLADAFAAGEVS
                     RAHAEVMTKAFTTERSGAMAELEPLLVDIAKETNARGLGEAVQRVTDTIDGDGGAAAD
                     AAKFERRRLHASVTIDRMVATDGMFDQLGGEIVLTALKAEMRRDHQPCDPRTAAQRRA
                     DAFVNICRRALDNGELGTTRRVRPHVTVIVDAHKTQDGWELIAEFANTGAISRATLGR
                     VLGDCSLARVITNGASQVLDVGRATRQVSRAQWNAVVARDRECQGCGRQPGDCQVHHK
                     NWWSSGGETNVAELELRCDFCHHKLHDRNPIRRE"
     gene            complement(1471..1716)
                     /locus_tag="VFX21_06305"
     CDS             complement(1471..1716)
                     /locus_tag="VFX21_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095603.1"
                     /translation="MSTLTAKQRANLEPKDFGLPEKARTKKARKQSGNYPIPDKKHAI
                     SALTRSERERERGNLTKDEYNRIQRKAKKKLKKTKKK"
     gene            complement(1830..1902)
                     /locus_tag="VFX21_06310"
     tRNA            complement(1830..1902)
                     /locus_tag="VFX21_06310"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1867..1869),aa:Arg,seq:cct)
     gene            complement(2107..2466)
                     /locus_tag="VFX21_06315"
     CDS             complement(2107..2466)
                     /locus_tag="VFX21_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX5095604.1"
                     /translation="MRIYTYSAEVMPTSASTTLPDLTGFEVEAEDGKIGKIDEATYEA
                     DASCVVVDTGFWIFGKKRMIPAGVVDRVDANDKTVFVRMTKDQIKNAPDYDPDRYQND
                     AQDYRREVGDYYTPYSS"
     gene            2592..>3769
                     /locus_tag="VFX21_06320"
     CDS             2592..>3769
                     /locus_tag="VFX21_06320"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEX5095605.1"
                     /translation="MPALHWPYRLIALAVVVVGTATLASCSSSDKSDANKKSSDTTAP
                     KVAFAAHVPTEITAPPAKGAMNKPQPAVAPAKNYVEEEYFVGGTATSFAAKDTPESGA
                     WNATPGAEKEYRTRVIVRRPANAADFSGTVLVEWFNVSALEASPDWAFLNTEIARAGH
                     AYIGVSAQKQGVEGGDTLLEVEVDPEQANSLAEGVPDKSGLKKIDPARYGTLVHPGDA
                     YSYDIFSQVARSVEKEPAKLLGALKPKQVIGVGESQSAAFLTTLVNAVHPLAPSFDGF
                     LIHSRGANGAPLDGKITRGSGSNDESSGIASRAIHIRTDLDVPVFMFETETDLTLLQY
                     ARARQPDTDQVRTWEVAGTSHADAHFIRSIVGGPRDPNVGSLLNCDAPINTGPQHEVL
                     "
BASE COUNT          655 a         1175 c         1266 g          673 t
ORIGIN      
        1 ccgacagcgc gcccgatcga acgcgggtcg ccaggtgccg ttgtgaccca cctgtgaccg
       61 ctgcggagat tcgccggcgc aagaagcgcc cgccacatgg gactacgcgg tggagacgat
      121 gggactcgaa cccacgaccc cctgcttgca aagcaggtgc tctagccagc tgagctacgt
      181 ccccgagggc cgttcatcgt atccgaggcg cttcgagatc aacgacgtga tcggtgcttg
      241 ccgaaaggcg ttcgcgtttc gaagaaacgc ttgcatggtc gttgccggtg gcgtaggcta
      301 cgaacaccag ttcgttccgc tcgggggacc ggagcgaacg aagggccggt cttccatgag
      361 tgcagcgttg gtcgtgcgcg acctcgacgc agcaatcgac aaagcattcg cctgcgaagg
      421 cgcgctcgat catgtcgagg ttgcgcgtct tgcggaccgg ttggagtgtt tgaagctgcg
      481 tgcgtttcgc gactacgcgc gttcggagca gtggaaggaa gaaggcttcg tcaccgccgc
      541 ggcggcagtc cgggcgcagt gccgcctcac cgaaggcgcg gcgcgccacg cattggatct
      601 gggacgcaag ctcgaagagc tgccggttct ggcggatgcg ttcgcggcgg gggaggtgtc
      661 gcgcgcccac gccgaagtga tgacgaaggc attcacgacc gagcgatccg gggccatggc
      721 cgagttggaa ccgttgctgg tggatatcgc gaaggagacc aacgcgcgcg ggcttggtga
      781 agccgtgcag cgggtcaccg acacgatcga cggcgatggg ggcgccgcgg ccgacgccgc
      841 caagttcgag cgccggcggc tgcacgcgtc ggtgacgatc gaccgcatgg tggccaccga
      901 cggcatgttc gaccagttgg gcggcgagat cgtgctgacg gccctgaagg ccgagatgcg
      961 gcgcgaccac caaccgtgcg acccgcgcac ggccgcgcag cggcgcgccg atgcgttcgt
     1021 gaacatctgc cgccgcgcgc tcgacaacgg tgaactcggc acgacgcgcc gggtgcggcc
     1081 ccatgtgacc gtcatcgtcg acgcgcacaa gacgcaggac ggctgggaac tgatcgcgga
     1141 gttcgcgaat accggcgcga tttcgcgggc gacgctgggg cgcgtgctcg gcgattgctc
     1201 gctggcgcga gtgatcacga acggcgcgtc gcaggtgctc gacgtcgggc gtgcgacgcg
     1261 ccaggtgtca cgggcgcagt ggaatgccgt cgtggctcgc gatcgcgagt gccaaggctg
     1321 cggccgtcag cccggcgact gccaggtgca ccacaagaac tggtggagca gcggcggcga
     1381 aacaaacgtt gccgaactgg agcttcggtg cgacttctgt caccacaagt tgcacgaccg
     1441 gaacccgatc cgtcgggaat gacgcagcga ctacttcttc ttcgtcttct tgagcttctt
     1501 cttggccttg cgctggatgc ggttgtattc gtccttggtc aagttgccgc gttcgcgctc
     1561 gcgttccgac cgcgtgagtg cgctgatcgc atgcttcttg tccgggatcg gatagttccc
     1621 cgactgcttg cgcgctttct tcgtgcgcgc cttttcgggc aacccaaagt ccttcggttc
     1681 gagattcgca cgctgtttcg cagtgagagt gctcatgtgc tcgcggtacc cggtcggagc
     1741 gctgcacatg cgcgaccgcc gggggtcaga tgtcagcgtc gtattcaggt gcggtcggca
     1801 cggcacgctc caacgtcgat gttgctgcgc gcccccggca ggattcgaac ctgcggcaca
     1861 cggtttagga aaccgatgct ctatcccctg agctacgagg gcggaggggt cattatcgca
     1921 ggtcgctccg tcagcgctcg cgctaacgga gcagcaggat caagacgacg atcaccagga
     1981 tgagtacgac agctccggcg ccgatataca tgatgagttg ttacccaaat cgtgctcgac
     2041 tgatgtttaa aacgaagaag gccgccggtt taaaaccggc ggccttctcg atcgaaccct
     2101 ggtcagctat gagctgtagg gcgtgtagta gtcaccgact tcgcgtcggt agtcctgcgc
     2161 gtcgttctgg tagcgatcgg ggtcgtagtc gggcgcgttc ttgatctgat ccttcgtcat
     2221 gcgcacgaaa acggtcttgt cgttcgcgtc aacgcggtcg acgacaccgg ccgggatcat
     2281 gcgcttcttg ccgaagatcc agaacccggt gtctacgacg acgcagctgg cgtcggcttc
     2341 gtaggtggct tcgtcgatct tgccgatctt gccgtcctcg gcttcgacct cgaaccctgt
     2401 gagatcggga agcgtcgtgc tcgcggacgt cggcatgact tcggcggagt acgtatagat
     2461 cctcactggt acctccttgg caatccgttg ttggtgtacc cggagggtcc gcccacaacc
     2521 tcgacggtct gcgcggcgat tcgcgtcggc gctacggttt caccgatttg ttcgctgcac
     2581 ctgggggacg catgcctgcg ctgcactggc cgtatcggtt gatcgcgctg gcggttgtcg
     2641 tcgtcggcac ggcgacgctc gcaagctgct cctcgagcga caagtcggac gcgaacaaga
     2701 agtcgtccga cacgaccgca cccaaggtcg cattcgccgc gcacgtgccg accgagatca
     2761 ccgcgccgcc ggccaagggc gcgatgaaca agccgcagcc ggctgtggcg cccgcaaaga
     2821 actatgtcga ggaggagtac ttcgtcggcg gtactgcgac gagttttgcg gcgaaggaca
     2881 cgcccgagag tggtgcatgg aatgcgaccc cgggagccga gaaggagtat cggactcgcg
     2941 tcattgtccg ccggcccgcg aacgcggccg acttcagcgg caccgtgctc gtcgagtggt
     3001 tcaacgtcag cgcgctcgag gcgagtccgg actgggcatt cctgaacacc gaaatcgcgc
     3061 gcgccggcca cgcgtacatc ggcgtcagcg cgcagaagca aggggtcgag ggcggcgaca
     3121 ctttgctcga ggtcgaggtc gacccggaac aggccaactc gctggccgag ggcgtcccgg
     3181 acaagagcgg gctgaagaag atcgatcccg cccggtacgg cacgctcgtg catcccggtg
     3241 acgcgtactc ctacgacatc ttcagccagg tcgcgcggtc ggtcgagaag gagcccgcca
     3301 agttgctcgg cgcgttgaag ccgaagcagg tcatcggcgt cggcgagtca cagtcggcgg
     3361 cgtttctcac gacgctcgtc aatgcggtgc atcccctcgc gccgtcgttc gacggcttct
     3421 tgatccacag ccgcggcgcg aacggagcgc cgctcgacgg caagatcacg cgcggttccg
     3481 ggagcaacga cgagtcgtcg ggcatcgcgt cgcgcgctat ccacattcgc acggacctcg
     3541 acgtgccggt cttcatgttc gagaccgaaa ccgacttgac gttgctgcaa tatgcgcgag
     3601 ctcgccaacc cgatacggat caggttcgga catgggaagt tgccgggacg tcgcacgccg
     3661 atgctcactt catccgatcg atcgtcggcg gaccgcgcga cccgaacgtc gggtcgctgc
     3721 tcaactgcga cgccccgatc aacaccggtc cgcagcacga agtgctgca
//
LOCUS       DASUGA010000321         7355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1126037, whole genome shotgun sequence.
ACCESSION   DASUGA010000321 DASUGA010000000
VERSION     DASUGA010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7355
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126037"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFX21_06325"
     CDS             complement(<1..401)
                     /locus_tag="VFX21_06325"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5095606.1"
                     /translation="MDFNDSPEEAAWRAECDAWLDEYAPTVAGPPAAAPMEPGSDEYM
                     QRAKRWQALKFDHGFARITWEPEFGGRNGTSMQQIIFGQEEAKYAVPGEAFIIGMGMI
                     APTLRACGTDAQKERYLTKLLRGEEIWCQLF"
     gene            complement(417..2915)
                     /locus_tag="VFX21_06330"
     CDS             complement(417..2915)
                     /locus_tag="VFX21_06330"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF009687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX5095607.1"
                     /translation="MGLDKTPELADIEERCRTLWEESGIYEFDPDAPAESIYSIDTPP
                     PYVSASHLHVGHAMSYAQAEFIIRYQRMRGRHVFYPMGFDDNGLPTERFVEQKYNIDK
                     SRTTRSEFRELCLKETAEIAAGYERFWRKLGLSVDWSLRYSTIDDHCRRTSQLSFLDL
                     YAKGRIYRSEEPVFWDTAMQTSLAQADLETITRGSTLHDIAFAAPDGRDLIISTTRPE
                     LIPSCVALYFHPEDDRYRSSQNGRAITPVTRHEVQILSDEDVKPDFGTGLMMVCTFGD
                     AMDVQRWKRDGLELRMGLDPAGRMTAVAGPYEGLSTEEARKRILVDLDAAGALRGSQK
                     IEQQVSVGERSQRPIEFQMRPHWFIRVLDMEPELLARSAELTWYPDYMKVRLDHWIEG
                     LKYDWNITRQRFYGVPFPIWYCTGCATPIVATEAQLPVDPLEDAPPVDTCPDCGGTEF
                     TGDPDVMDTWMTSSLTPQINANWAQTPGRTGVNGLPMTLRVQAFEIIRTWLFYSVVKA
                     HVHNDAVPWTDVMISGWGLNEQGKKFSKRDLEAKKPGQYSKYDPEDVIDKHGADALRY
                     WAAGSHLGHDLRYHEEDVKAGRKLVLKLWNVTRLAEQYGEGYDLASAAPVATRTPEDR
                     WIINRLEQVLPVVERGFETYDYAIAREALDRFFWDDFCDDYLEIVKDRFWSEERYTDE
                     QRDSARATMWETLRAVLGLYAPFVPFITEELYQYRFRAHEGAPSIHVTAYPQPPVSPT
                     AEVPEIETLREVLRAVRSERTRLRIPQGRPLTEMVIDTSGAAPELRAAIEGLTDSIAA
                     AARARVVRFDSAEAASTIDGVRIDIVPEEKREAS"
     gene            3007..4173
                     /locus_tag="VFX21_06335"
     CDS             3007..4173
                     /locus_tag="VFX21_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5095608.1"
                     /translation="MVMVPFDPTPVEDPAELGFDPDAITRLVERAQREIDDGVLPGCQ
                     FSLARNGRVGFTIALGDAKTDDRFIMYSATKALVASAVWILIGDGSIDVSRRVVEYIP
                     EFGTNGKDVITIEQVMLHTSGFAYAPFNALDWDNRERRYERFAQWRCNWEPGTRYVYH
                     ATSAHWVLAELIERASGTDFREFIRTRVIEPLGLQRLALGVPLEQQGDINEVVLTGEP
                     LSEDELLAIIGVREMPFTDVTPELLISLNQPPARAVGVPGGGGVSTSGDVALFYQALL
                     HNPASIWKPDVLADVTTHVRCTLKDWLGTPANRSLGLILAGDDGKSNLRGMGRTVSPG
                     AFGHNGAGGQIAFADPATGLSFAYLTNGLDQNVVREARRNTAIASRAADCVASA"
     gene            4278..5492
                     /locus_tag="VFX21_06340"
     CDS             4278..5492
                     /locus_tag="VFX21_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095609.1"
                     /translation="MDVRRIGIWMVAVGGGALSIGLVLDAIDNADDPSRAAKEGIFDL
                     SSFPHALFFGGICVAILGLFAILFGGRLYEHTDRVTVGRRMAQVGVPIAAVVLIIGAA
                     AFAGNSGLSEGNDERTVSTEPASSSSDAQTATDDHSAEHDMSQMAPGEEHADEHAAGE
                     HDMAGMGTVIEGTATGDSPCEVAQPTPASPGQVGAGGGGSSEGVIGEHGHRGMVQQEP
                     LTQEQRTELQSQMEAARSVAVTYPTVAAAEAGGYHKSTPYVPCIGAHYTNFGLVGSFD
                     PAKPSELLFDGTNPDSKIVGLSYLVYNKGGAPEGFAGPNDHFHQHNANGGLCLQGGLV
                     VGGEEVSPEECAQRGGKKQELTDIWMEHAWIVPGFECSWGVFAGECPELGGRTGGTAW
                     DAPAPAESTASN"
     gene            complement(5514..6212)
                     /locus_tag="VFX21_06345"
     CDS             complement(5514..6212)
                     /locus_tag="VFX21_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095610.1"
                     /translation="MLLLHEVHTLVGPHEDDFEASVREEYMPTVAEDDDARLLYYLKL
                     AHGTGRAYHATTITALRDGAAYERLASRVQTGDLRKWAAKVDGFRHEVAGKLLVPVEW
                     SPLEIDFATVPTDARTHACELFMEDSAWPYEAKLDDYLDAARTHYAPSLEQRTERSLL
                     TLLGVFQAALGAPRRREVVLWQRVDFPERLPALFTRELPAHVKAPGTWMHDALQVRDD
                     WESRLLRSAGWSPW"
     gene            complement(6219..6743)
                     /locus_tag="VFX21_06350"
     CDS             complement(6219..6743)
                     /locus_tag="VFX21_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_273701375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6459..6461),aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HEX5095611.1"
                     /translation="MNPNRVLMDPTGERQPATRERVRRPPSLEGKNVGLLDITKPRGD
                     VFLDRLEDLLVQRGATVERFRKPTFTKPAPVDLRHEIATNCDVVIEALADUGSCTSCS
                     VHDTVNLEAAGVVTVFVASTEFVDAADAQSRSLGADPARVFVPHPIQDRTDEELRALA
                     DDALTALLEAITET"
     gene            complement(6740..>7355)
                     /locus_tag="VFX21_06355"
     CDS             complement(6740..>7355)
                     /locus_tag="VFX21_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX5095612.1"
                     /translation="QLVIRNVGGGRPGEVDRATLGQPGKYTFCFAEDEQGSPWEPMHV
                     ERGLQSGQSTVTLFAGEGVRGIVDQLSREPESLARSFAACLRTVAHPKNVIIWDAVLV
                     VSPEHGRVFRDAGWTKARLREELDTLLQIPGEEFVRGAGGIAEGMPEGFREMTLPKFR
                     DGGLLIVHAGGGAGLFSGIIGGWASGAVGSAPVTHPIETAEVSV"
BASE COUNT         1119 a         2509 c         2501 g         1226 t
ORIGIN      
        1 ctgaagagct ggcaccagat ctcctcgccg cgcagcagct tggtgagata ccgctccttc
       61 tgcgcatcgg tgccgcacgc acgcagcgtc ggggcgatca tgcccatgcc gatgatgaac
      121 gcctcgcccg gcacggcgta cttcgcctcc tcctgcccga agatgatctg ttgcatcgac
      181 gtgccattgc ggccgccgaa ctccggctcc caggtgatcc gcgcgaaccc gtggtcgaac
      241 ttcagcgcct gccaccgctt ggcgcgctgc atgtattcgt cgctgccggg ttccatcggc
      301 gcggccgccg gcggtcccgc cacggtcggc gcgtactcgt cgagccaggc gtcgcactcc
      361 gcccgccaag cggcctcttc ggggctgtcg ttgaagtcca tcttgcgaac tcctcgctag
      421 ctggcctcgc gcttctcttc gggcacgatg tcgatccgca cgccgtcgat cgtcgatgct
      481 gcctcggcgc tgtcgaagcg cacaacacgg gcgcgtgccg cggccgcgat gctgtcggtc
      541 agcccttcga tcgcggcccg cagctcgggc gcggcgccgg atgtgtcgat gaccatctcg
      601 gtcagcgggc ggccctgcgg gatgcgcagg cgggtgcgct ccgaccgaac cgcgcgcagc
      661 acttcgcgca acgtctcgat ctccggcact tcggcggtcg gcgacacggg cggctgcggg
      721 tacgcggtga cgtggatcga cggcgcacct tcgtgcgcac ggaagcggta ctggtacagc
      781 tcctcggtga tgaacggcac gaacggcgcg tacaagccga gcaccgcgcg caacgtctcc
      841 cacatcgtcg cccgcgccga gtcacgctgt tcgtcggtgt agcgctcctc gctccagaag
      901 cggtccttga cgatctcgag gtagtcgtcg cagaagtcgt cccagaagaa gcggtcgagc
      961 gcctcacgcg cgatcgcgta gtcgtaggtc tcgaagccac gctctacgac cgggagcacc
     1021 tgttcgagcc ggttgatgat ccagcggtct tcgggcgtac gggtcgcgac cggtgccgcg
     1081 ctcgcgaggt catagccctc gccgtactgc tccgcgagcc gcgtgacgtt ccacaacttg
     1141 agcacgagct tgcggcccgc cttcacgtct tcttcgtggt agcgcaggtc gtggccgagg
     1201 tggctgcccg cggcccagta gcgcaacgcg tcggcgccgt gcttgtcgat gacgtcttcg
     1261 gggtcgtact tggagtactg cccgggcttc ttcgcttcga ggtcgcgctt ggagaacttc
     1321 ttgccctgct cgttgaggcc ccagcccgag atcatcacgt cggtccacgg cacggcatcg
     1381 ttgtgcacgt gcgccttcac aacggaatag aagagccacg tgcggatgat ctcgaaggcc
     1441 tgcacgcgca gtgtcatcgg cagcccgttg acacccgtgc gccccggcgt ctgcgcccag
     1501 ttggcgttga tctgcggcgt gagcgacgac gtcatccacg tgtccatgac gtcggggtcg
     1561 ccggtgaact cggtgccgcc gcagtcggga caggtgtcga ccggcggcgc gtcttcgagc
     1621 gggtcgaccg gcaactgcgc ttcggtcgcg acgatcggcg tcgcgcaacc ggtgcagtac
     1681 cagatcggga acggcacgcc gtagaagcgc tggcgcgtga tgttccagtc gtacttgagg
     1741 ccctcgatcc agtggtcgag gcgcaccttc atgtagtcgg ggtaccacgt cagctccgcg
     1801 ctgcgcgcga gcagctcggg ctccatgtcg agcacgcgga tgaaccagtg cggacgcatc
     1861 tggaactcga tcgggcgctg cgaacgctca ccgaccgaca cttgctgctc gatcttctgc
     1921 gaaccgcgca acgcgccggc cgcgtcgagg tcgacgagga tgcgcttgcg tgcctcttcg
     1981 gtcgacaacc cttcgtacgg gcccgcgacc gcggtcatgc gccccgcggg gtcgagaccc
     2041 atgcgcaatt cgaggccgtc gcgcttccat cgctgcacgt ccatcgcgtc accgaacgtg
     2101 cagaccatca tcaacccggt gccgaagtcg ggcttgacgt cttcgtcgga gaggatctgc
     2161 acctcgtggc gggtcacggg tgtgatcgcg cggccgttct gcgatgaccg gtaacggtcg
     2221 tcttcggggt ggaagtagag cgcgacacaa ctcgggatca gctcgggccg tgtcgtcgag
     2281 atgatcaggt cgcgaccgtc gggcgcggcg aacgcgatgt cgtgcagcgt cgagccgcgg
     2341 gtgatcgtct cgaggtcggc ttgcgcgagc gacgtctgca tggcggtgtc ccagaacacc
     2401 ggctcctcgg agcgatagat acggcccttc gcgtagaggt cgaggaacga gagctgcgac
     2461 gtgcggcggc agtggtcgtc gatcgtcgag taccgcagcg accagtcgac cgacaggccg
     2521 agcttgcgcc agaaccgctc gtaaccggcc gcgatctcgg ccgtctcctt gaggcacagc
     2581 tccctgaact ccgagcgcgt cgtgcgcgac ttgtcgatgt tgtacttctg ctcgacgaag
     2641 cgctcggtgg gcaggccgtt gtcgtcgaag cccatcgggt agaagacgtg acggccgcgc
     2701 atgcgctggt agcggatgat gaactcggcc tgcgcgtacg acatcgcgtg gccgacgtgc
     2761 aggtgggacg ccgagacgta cggcgggggc gtgtcgatcg agtagatgct ctcggccggc
     2821 gcgtcggggt cgaactcgta gatgccggac tcttcccaga gcgtgcggca ccgctcctcg
     2881 atgtcggcaa gttcgggcgt cttgtcgagg cccatggcgg cgggtcccac ccttcagcac
     2941 agaggttcgt ggagcgacgg agcttagttc tggccgcggc tcgaccttgc gtggcaacct
     3001 tcggccatgg tcatggttcc gttcgatccc acacccgtcg aggaccctgc cgagctgggc
     3061 ttcgaccccg acgccatcac tcgtctcgtg gagcgcgcgc aacgcgagat cgacgacggc
     3121 gtgctccccg gctgccagtt cagcctggct cgcaacggcc gcgtcggctt cacgatcgcg
     3181 ctcggtgacg cgaagaccga cgaccggttc atcatgtact ccgcgacgaa ggcactcgtc
     3241 gcgtcggcgg tgtggatcct gatcggtgac ggctcgatcg acgtgtcgcg tcgcgtcgtc
     3301 gagtacatcc ccgagttcgg tacgaacggc aaagacgtca tcaccatcga gcaggtgatg
     3361 cttcacacgt cggggttcgc gtacgcgccg ttcaacgcgc tcgactggga caaccgcgaa
     3421 cggcgatacg aacggttcgc gcaatggcgg tgcaactggg agccggggac gcgctacgtg
     3481 tatcacgcca cctcggcgca ctgggtgctc gccgagttga tcgagcgcgc gtcgggcacc
     3541 gacttccgcg agttcattcg cacacgcgtc atcgaaccgc tcggactgca acggctcgcg
     3601 ctcggtgtgc cgctcgaaca acagggagac atcaacgaag tcgtgctcac cggcgaaccg
     3661 ctgagcgagg acgaactgct cgcgatcatc ggggtgcgcg agatgccgtt caccgacgtc
     3721 acgccggagc tgttgatctc gttgaatcag ccgcccgccc gcgccgtcgg tgtgcccggc
     3781 ggcggcggtg tgtcgacgtc aggcgacgtc gcgctcttct accaagcgct gttgcacaac
     3841 cccgcgagca tctggaagcc cgacgtgctc gcggatgtca cgacgcacgt gcgctgcacg
     3901 ttgaaggact ggttgggcac ccccgcgaac cgttcgctcg gcctgatcct cgcgggcgac
     3961 gacggcaagt cgaacctgcg cggcatgggc cgcacggtct cgcccggcgc gttcgggcac
     4021 aacggcgccg gcggccagat cgccttcgcg gatcccgcga ccgggctctc gttcgcctac
     4081 ctcaccaacg gcctcgacca gaacgtcgtg cgggaggccc ggcgcaacac cgcgatcgcg
     4141 agccgcgccg ccgactgcgt ggcttcggcg taggatccct tttatcagtt ttgcccgggt
     4201 gaccggcacg gctcgtacag gccgtcgtaa ggttgcacgg tcagtgcagt ttcggtcggg
     4261 ggatcggggt caacgccgtg gacgttcgca ggatcggcat ctggatggtc gcggtcggtg
     4321 gaggagcgct ctccatcggg ctcgtgctgg acgcgatcga caacgccgac gatccgagtc
     4381 gcgcggccaa ggaaggcatc ttcgatctgt cgagcttccc gcacgcgttg ttcttcggcg
     4441 gcatctgcgt cgcgatcctc ggtctgttcg cgattctctt cggtggtcgc ttgtacgagc
     4501 acaccgatcg cgtcaccgtc ggccggcgca tggcgcaagt cggtgtgccg atcgccgccg
     4561 tcgtgttgat catcggtgcc gcggcgttcg cggggaactc cggactctcc gaaggcaacg
     4621 acgagcgcac ggtctcgacc gaaccggcat cctcgtcgag cgacgcgcag accgcgaccg
     4681 acgaccactc cgccgagcac gacatgtcgc agatggctcc cggcgaagag cacgccgacg
     4741 aacacgccgc gggcgagcac gacatggcgg gcatgggcac ggtgatcgaa ggcaccgcga
     4801 ccggcgactc gccgtgcgag gtcgcgcagc cgacgcccgc gtcaccgggc caggtcggcg
     4861 ccggtggcgg cggcagctcc gaaggcgtga tcggtgagca cggtcaccgc ggcatggtgc
     4921 agcaagagcc gctcacgcag gagcagcgca ccgagctcca gtcgcagatg gaagccgcgc
     4981 gcagcgttgc ggtgacgtac ccgaccgtcg ccgccgccga agccggcggt taccacaagt
     5041 cgacgccgta cgtgccgtgc atcggtgcgc actacaccaa cttcggtctc gtcggttcgt
     5101 tcgatcccgc gaagccgtcc gagttgctct tcgacggcac gaaccccgac tccaagatcg
     5161 tcggcctgag ctacctcgtg tacaacaagg gcggtgcgcc cgagggcttc gccgggccga
     5221 acgaccactt ccaccagcac aacgcgaacg gcggtctgtg cctccagggt ggcctggtcg
     5281 tcgggggcga ggaggtctcg cccgaggagt gcgcccagcg cggcggcaag aagcaggagc
     5341 tcaccgacat ctggatggag cacgcgtgga tcgtgcccgg cttcgagtgc agctggggcg
     5401 tgttcgccgg tgagtgcccc gagctcgggg gccgcaccgg tggcaccgcc tgggacgcac
     5461 cggcaccggc cgagtcgacc gcttcgaact aacccgtgct ccggacggag cacttaccac
     5521 gggctccagc ccgcgctgcg gaggagacga ctctcccagt cgtcgcgcac ttggagcgcg
     5581 tcgtgcatcc acgtccccgg cgccttcacg tgcgcgggca gttcacgcgt gaagagggcg
     5641 ggcaaccgct cggggaagtc gacgcgctgc cacagcacga cttcacgccg gcgcggtgcg
     5701 cccagcgcgg cctggaacac gccgagcaac gtgagcagcg agcgttcggt gcgttgctcg
     5761 agactcggcg cgtagtgcgt gcgcgccgcg tcgaggtagt cgtcgagctt cgcctcgtat
     5821 ggccacgcgc tgtcctccat gaacagctcg caggcgtgcg tgcgcgcgtc ggtcgggacg
     5881 gtcgcgaagt cgatctcgag cggtgaccac tcgacgggta ccaggagctt gcccgcgacc
     5941 tcgtgacgga acccgtcgac cttcgccgcc cacttccgca ggtcgcccgt ctgtacgcgg
     6001 gacgccagtc gctcgtacgc ggcgccgtcg cgcagcgcgg tgatcgtcgt cgcgtgatac
     6061 gcccggccgg tgccgtgcgc gagcttcagg tagtagagca agcgcgcgtc gtcgtcctcg
     6121 gcgacggtgg gcatgtactc ctcgcgcacc gacgcctcga agtcgtcttc gtgggggccg
     6181 acgagcgtgt ggacttcgtg caggagcagc attggcggtt acgtctccgt gatcgcttcc
     6241 aacaacgcag tcaaggcgtc gtcggccagc gcgcgcaact cctcatcggt gcggtcctgg
     6301 atcgggtgcg gcacgaacac gcgcgccgga tcggcgccga gcgaacgcga ctgcgcgtcg
     6361 gcggcgtcga cgaactccgt cgacgcgacg aacaccgtca cgacgccggc cgcttccaga
     6421 ttgacggtgt cgtgcacact gcacgacgtg cacgagcctc aatccgcgag cgcttcgatc
     6481 acgacgtcgc aattggtcgc gatctcgtgg cgcaggtcga ccggcgcggg cttggtgaac
     6541 gtcggcttgc ggaagcgttc gaccgtggcg ccccgctgca cgagcaggtc ctcgagccgg
     6601 tcgaggaaca cgtcgccgcg cggcttggtg atgtcgagca ggccgacgtt cttgccctcg
     6661 agcgacggtg gccgccgcac gcgctcgcgg gtcgcgggct gccgctcacc cgtcgggtcc
     6721 atcaacactc ggttcgggtt cataccgaca cctccgccgt ctcgatcggg tgcgtgaccg
     6781 gcgcgctgcc gacggccccg ctggcccacc ccccgatgat gcccgagaac aggccggccc
     6841 cgccgccggc gtgcacgatc aacagcccgc catcgcggaa tttcggcagc gtcatctcgc
     6901 ggaagccttc gggcatccct tcggcgatgc cgcctgcgcc ccgcacgaac tcctcgccgg
     6961 ggatctgcag cagcgtgtcg agctcctccc gcagccgggc cttcgtccag cccgcgtcgc
     7021 ggaagacgcg cccgtgctcg ggcgagacga cgagcactgc gtcccagatg atcacgttct
     7081 tcgggtgcgc gaccgtgcgc aggcacgcgg cgaacgaacg cgcgagcgac tccggctcgc
     7141 gcgacagctg gtcgacgatg ccgcgcacgc cttcgcccgc gaacagggtc accgtggact
     7201 gaccggattg gagaccgcgc tcgacgtgca tcggttccca cggggatccc tgctcgtctt
     7261 cggcgaagca gaacgtgtac ttgccgggtt ggcccagcgt ggcccggtcg acttcgccgg
     7321 gtcggccacc gccgacgttg cggatgacga gctgc
//
LOCUS       DASUGA010000322         2725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1311139, whole genome shotgun sequence.
ACCESSION   DASUGA010000322 DASUGA010000000
VERSION     DASUGA010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311139"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VFX21_06360"
     CDS             complement(<1..831)
                     /locus_tag="VFX21_06360"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX5095613.1"
                     /translation="MTDSLYIIPEVPEPVAPRRGLARDISLSTVGSFAGKLLGAAGGV
                     VTARLLGPAGKGDYALFALLGAAVGTAATMGFQFWIAREVARPDGLDVPRASSAVRRQ
                     SGLAAAAIAALLLLIGGPLVALDAVSAADAFAVAALAWASVVSVLALAVPNGQRRMGI
                     VAWAFGAGGALYLGTACALLAADRASVAAVLFIAAVGNLATALVALPRVLRPGTRVAL
                     PQGWYRDAMRLGVPGALGELVLLATFRIDLLIIAAFGNATEVGLYAVASSLAELLWLV
                     P"
     gene            complement(844..2157)
                     /locus_tag="VFX21_06365"
     CDS             complement(844..2157)
                     /locus_tag="VFX21_06365"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX5095614.1"
                     /translation="MRRRLAFLLVQLITIASLATTVAIHAARHDYSATGTERFAWAVA
                     YLVVLVLASYAAGIPDAIDDRSPVLTSLVAATAGALAMSGVQLVVGSAVLPRFVVFGT
                     ACLVTVGWTIVTDLFQRGPDHAGEIDRVVFVGGGLDATAILKDVQLAPERRAKVVATV
                     LPAEIRPIARGEEPLVDLAIDDDATVIVLGRDVQDDRAVVVQASALHESGVRVRSITD
                     FYDEWLGKLPAFELQRMSLMFDISEIHAQSYARVKRLFDFCIGLVGCVVLVPVIPFVW
                     LVNLAANRGSLFYRQPRVGRAGVAFDILKFRTMKPGGSSEWTSEDDPRITPFGRVMRR
                     MHIDELPQFWNIVRGDLSIVGPRPEQPRYVAELVDCLPYYKMRHLVRPGLTGWAQVKY
                     GYAGNQNDALEKLQYDFYYLGHQSLRLDARIVARTIRSTVGRGGR"
     gene            complement(2157..>2725)
                     /locus_tag="VFX21_06370"
     CDS             complement(2157..>2725)
                     /locus_tag="VFX21_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095615.1"
                     /translation="WRDGRRRLLAIVALAALPLVWMSQYVGGAYPQWGGRYVLATSIL
                     LGVIGLVAAMDLVPVLAKGAIVLSLVITLAGVVNLSFRSHDADRVGKLVTELPQDVVI
                     ARNGFLMREFGAAYDETRPWLTAYNDDLLTKAFGVAEARDADTVALLTMGASEFATPK
                     IDGWKVTTRQKWDVFYQRLDVVTYERAG"
BASE COUNT          516 a          939 c          916 g          354 t
ORIGIN      
        1 gggcacgagc cacaacagtt cggcgagcga cgaggcaacc gcgtagaggc caacctcggt
       61 ggcgttgccg aaggcggcga tgatcaagag gtcgatccgg aacgtcgcga gcagcacgag
      121 ctcgccgagc gcgccgggca cgccgaggcg catcgcgtcc cgataccagc cttgcggcag
      181 cgcgactcgc gttcccggcc gcagcacgcg cggcaacgcg acgagcgcgg tcgcaaggtt
      241 gcccaccgcc gcgatgaaca acacggccgc gaccgacgct cggtccgcgg caagcaacgc
      301 gcaggcggta ccgaggtaca gcgcaccacc ggcgccgaag gcccacgcga caatccccat
      361 gcgccgctga ccgttcggca ccgcgagcgc gagcacgctg acgaccgaag cccaggcgag
      421 tgccgcgacc gcgaacgcat ccgcggccga gacggcatcg agcgcgacga gcggcccgcc
      481 gatcagaagc aggagcgccg cgatcgcggc ggccgcgagt cctgattgcc gccggaccgc
      541 gctcgacgcg cgcggcacat cgagaccgtc gggccgggca acttcgcgcg cgatccagaa
      601 ctggaagccc attgtggcgg ccgttccgac cgcggcgccg agcaacgcga agagggcgta
      661 gtcgcctttg ccggccggac cgaggagacg ggcggtgacg acgccgcccg cggcaccgag
      721 cagcttgccg gcgaacgatc cgacggtcga cagcgagatg tcgcgcgcga gtccgcggcg
      781 cggagcaacc ggttcaggaa cctcgggaat gatgtagagc gagtcagtca cgaccgcgcc
      841 gcgtcagcgg cctcctctgc cgacagtgct ccgaatcgta cgcgccacga tgcgcgcatc
      901 gagacgaagg ctctgatgac cgaggtaata gaagtcgtac tggagcttct ccaacgcgtc
      961 gttctgattg cccgcgtagc cgtatttcac ttgcgcccag ccagtgagac ccggccgcac
     1021 caagtggcgc atcttgtaat acggcaggca gtcgacgagt tctgccacgt agcgcggttg
     1081 ctccggccga ggaccgacga tcgacagatc accgcgcacg atgttccaga actgcggcag
     1141 ctcgtcgatg tgcatgcgac gcattacgcg accgaacggc gtgatccgcg gatcgtcctc
     1201 gctggtccac tcactcgaac cgcccggctt catcgtgcgg aacttcagaa tgtcgaacgc
     1261 gacaccggcc cggccgacgc gcggctggcg gtagaacagc gaaccgcggt tcgcggccag
     1321 gttgaccaac catacgaacg ggatgacggg cacgagcacg acgcaaccga cgaggccgat
     1381 gcagaagtcg aacaagcgct tcacgcgggc gtagctctgg gcatggatct cgctgatgtc
     1441 gaacatcagc gacatccgct gcagctcgaa cgccggcagc ttgccgagcc attcgtcgta
     1501 gaagtcggtg atcgagcgaa cgcgcacgcc ggattcgtgc aaggccgatg cctgcaccac
     1561 gacggcgcga tcgtcctgga cgtcgcgacc gagcacgatg accgtcgcgt cgtcgtcgat
     1621 cgccaggtcg acgagtggct cttcaccgcg cgcgatcggc cggatttcgg cgggcagcac
     1681 ggtcgccaca accttcgccc tgcgttcggg cgcaagctgg acgtctttca gaattgcggt
     1741 cgcgtccagt cccccgccca cgaacacgac gcggtcgatc tcaccggcgt gatcgggccc
     1801 ccgttgaaag aggtcggtga cgatggtcca gccgaccgtc accaggcacg cggtgccgaa
     1861 gacgacgaac cgcggcaaca ccgccgagcc gacgacgagt tgcacgcccg acatcgcgag
     1921 cgcgccggcg gtcgcggcaa cgagcgacgt gaggaccggg ctgcgatcgt cgatggcatc
     1981 cgggatgcct gcggcgtacg acgcgaggac gagcacgacc aagtaggcaa ctgcccacgc
     2041 gaaccgttcg gtaccggtgg ccgagtagtc gtgccgcgcc gcgtggatcg cgaccgtcgt
     2101 ggccagcgac gcgatcgtga tcagctgaac cagcaggaac gccaggcggc ggcgcatcag
     2161 ccggcccgct cgtacgtcac gacatcgagc cgctgataga agacgtccca cttctgccgg
     2221 gtcgtgacct tccagccgtc gatcttcgga gtcgcgaact cggatgcgcc catcgtgagg
     2281 agcgcgaccg tgtcggcgtc gcgtgcttcc gcgacaccga acgccttcgt gagcagatcg
     2341 tcgttgtatg ccgtcagcca cgggcgcgtc tcgtcgtacg cggccccgaa ctcccgcatc
     2401 aagaacccgt tgcgtgcaat gacgacgtcc tgcggcaact cggtgacaag cttgccgacc
     2461 cggtcggcgt cgtgcgaccg gaaggagagg tttacgacac cggccagggt gatcacgagc
     2521 gaaagcacga tcgcgccctt ggccagcacc ggcacgagat ccatcgccgc gacgaggccg
     2581 atgacgccga gcaggatcga cgtcgcgagc acgtagcggc cgccccactg cgggtacgcg
     2641 ccgccgacgt actgcgacat ccacacgagc ggcaacgcgg ccagcgcaac gatcgcgagc
     2701 agccggcggc ggccgtcgcg ccatg
//
LOCUS       DASUGA010000323         2158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2043183, whole genome shotgun sequence.
ACCESSION   DASUGA010000323 DASUGA010000000
VERSION     DASUGA010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2043183"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..447)
                     /locus_tag="VFX21_06375"
     CDS             complement(7..447)
                     /locus_tag="VFX21_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095616.1"
                     /translation="MVWRVQPRGLGTMDEFMEIVRYFGTEDPDELLRRYDQALEMLAT
                     DDVDTWLNHSMDAGITEGALDHFRRDWVDGSEMPVEGSQITSALHDGFAAALSEARDN
                     GVRTSIVWVERGSEFTVDHVLGGNGVTVIVSVPEGTAAAQNAPS"
     gene            481..1815
                     /locus_tag="VFX21_06380"
     CDS             481..1815
                     /locus_tag="VFX21_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5095617.1"
                     /translation="MAGRTEYARIGDASVAYQVVGDGNLDLVFVPEWMTHLELQWQEP
                     ACARFLERLASFARLILFDKRGVGLSDPIAVGEGSGLDPWMDDVNAVLDHAKSQRAAF
                     VGTGAGGPMTMLYAASHPERVSALVCCNTGARFMQTDDYPYGAPAGIVEEAVQYTRDT
                     WGSGQLFPMGAPSLATSPAAREFHARFQRQAAGPGVAAEMQRMVLGIDVRPILSSIQV
                     PTLVVHRAEDALVDVAHGRYLAEHIPGATYVELPGEDHLYFAGDAGLLVQEIQTFVTG
                     KPVEVEPDRVLATVLFTDIVGSTDRAVELGDRRWRDLLDRHDALVREQLMVFRGREID
                     TAGDGFFATFDGPARALRAACAIRDAVRTIGLDIRVGVHTGEVDVRGDTYSGIGVHIG
                     ARVGALAEAGEVLTTKTVADLVAGSGIAFADRGTHRLKGVPDDWQLMAVAGF"
     gene            1817..>2158
                     /locus_tag="VFX21_06385"
     CDS             1817..>2158
                     /locus_tag="VFX21_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095618.1"
                     /translation="MLIPLRFREPIERGDVTLTFRRWKRSQVLAGRTYRTAVGRLVVE
                     SVTVVHPDKITTRDAKAAGFGSRAELVKELRGEEDWPTYRIQFHLAQDEDPRSALAAD
                     DALDEGEVAEID"
BASE COUNT          338 a          727 c          745 g          348 t
ORIGIN      
        1 tactaactag ctcggagcgt tctgggcggc ggcggtgcct tcgggaacgg agacaatcac
       61 cgtgacgccg ttgccgccca gcacgtggtc gaccgtgaac tccgagccgc gctcgaccca
      121 cacaatgctg gtacgcacgc cgttgtcgcg tgcttcgctc aacgccgcgg cgaagccgtc
      181 gtggagggcg ctggtgatct gcgagccttc gaccggcatc tcggaaccgt cgacccagtc
      241 gcgccggaag tggtcgagtg cgccttcggt gatgccggca tccatcgagt ggttgagcca
      301 cgtgtcgacg tcgtcagtcg cgagcatttc gagcgcctgg tcgtaacgac ggagcaactc
      361 gtcgggatcc tcggtgccga agtaccgcac gatttccatg aactcgtcca tcgttccgag
      421 tccgcggggt tgcactcgcc acaccatggc gctgaccgta gcgcggcgta gcctttgggc
      481 gtggcaggcc gaaccgaata cgcgcgcatc ggcgacgcat cggttgcgta tcaggtcgtt
      541 ggcgacggca acctcgacct cgtgttcgtg cccgagtgga tgacgcacct cgaactgcag
      601 tggcaagagc cagcatgtgc gcgattcctc gaacggctgg cgagcttcgc ccggctgatt
      661 cttttcgaca agcgcggcgt cgggctctcc gatccgatcg ccgtcggcga gggcagcggc
      721 ctcgatcctt ggatggacga cgtcaacgcg gtgctcgatc acgccaagtc gcagcgggcc
      781 gcattcgtgg gcaccggcgc gggcggaccg atgacgatgc tgtacgcggc atcgcatccc
      841 gagcgcgtca gtgcactcgt gtgctgcaac accggtgcgc ggttcatgca gaccgacgac
      901 tacccctacg gcgcgccggc cggcatcgtc gaagaagccg tgcagtacac acgcgacacg
      961 tgggggagcg gccagctgtt cccgatgggc gcgccgagcc tcgcgacgtc gccggccgcg
     1021 cgcgagtttc acgctcgatt tcagcgccag gccgcgggcc cgggcgtcgc ggccgagatg
     1081 caacgcatgg tgctcggcat cgacgtgcgc ccgatcctct cgtcgatcca ggtcccgacc
     1141 ctcgtcgtgc accgggccga ggatgcgctg gtcgacgtcg cgcacggccg ctacctcgcc
     1201 gaacacattc cgggcgcgac ctacgtcgag ctgcccggcg aagaccatct ttacttcgcc
     1261 ggcgacgcgg gcttgctggt gcaagagatt cagacgttcg tgaccggcaa gccggtcgag
     1321 gtcgaacccg accgcgtgct cgccacggtg ctgttcaccg acatcgtcgg ctcgaccgac
     1381 cgcgcggtcg aactcggcga ccggcgctgg cgcgacttgc tcgatcgcca cgacgcgctc
     1441 gtacgcgagc agctgatggt ctttcggggt cgcgagatcg acaccgccgg cgacggcttc
     1501 ttcgcgacgt tcgacggtcc ggcacgcgcg ctgcgggcgg cgtgcgcgat ccgcgacgca
     1561 gtgcggacca tcggactcga tattcgcgtc ggcgtgcaca cgggcgaagt cgacgtgcgc
     1621 ggcgacacct acagcggcat cggcgtgcat atcggcgcgc gcgtcggcgc gctcgcggag
     1681 gccggcgaag tgttgacgac gaagacggtg gccgatctcg ttgccggttc gggcatcgcg
     1741 ttcgccgacc ggggcaccca tcgcctcaag ggtgtgccgg acgactggca gctcatggcg
     1801 gtcgcagggt tctgacgtgt tgatcccgct gcggttccgc gagccgatcg agcgcggcga
     1861 cgtgacgctg acgttccggc gttggaaacg cagccaggtg ctcgcgggcc ggacctaccg
     1921 caccgcggtc gggcgattgg tcgtcgaatc ggtcacggtc gtgcaccccg acaagatcac
     1981 gacgcgcgac gcgaaagccg ccggcttcgg ctctcgcgcg gaactcgtca aggagttgcg
     2041 cggcgaggag gactggccga cgtaccgcat ccagttccac ctcgcacaag acgaggaccc
     2101 gcgcagcgcg ctcgcggccg acgacgcgct cgacgagggc gaggtcgccg aaatcgac
//
LOCUS       DASUGA010000324         3621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1850651, whole genome shotgun sequence.
ACCESSION   DASUGA010000324 DASUGA010000000
VERSION     DASUGA010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850651"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="VFX21_06390"
     CDS             <1..1204
                     /locus_tag="VFX21_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5095619.1"
                     /translation="LRGMVAVPLVYDPYDYEIDANPHPIWKRMRDEAPLYYNEQYDFY
                     ALTRFDDVLRAHLDAATFSSARTTVLELMGEAPAGFEDFMIFMDPPAHTRYRKLVSRA
                     FTPRHIAELEIRIRELCADFLDPLVGAGGFDYVADFGARLPVLVISALLGAPREDTKQ
                     LQEWSDATLHIDPGGMMGDAVAQINRQIHDYWQAHIDYRRKHPQDDIMGELMTAELET
                     ADGGARHLTDSELHGFYGLLSGAGNETVARFLGWAATGLDQFPAERAKLAADPELIPN
                     AVEEILRWEAPSAIQGRWVTEDVPLHGSVLPKNTKVALLTGAANRDERVFEKPDEIQV
                     ERDISRHVAFGYGIHFCLGAALARLEGRIALEETFKRFPTWTVDRDNCEMVHTSTVRG
                     YAKVPIST"
     gene            complement(1218..2363)
                     /locus_tag="VFX21_06395"
     CDS             complement(1218..2363)
                     /locus_tag="VFX21_06395"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5095620.1"
                     /translation="MAVSSLMVRDWHEWHRAYDSSDSPLSQRLRVVQQYIAEAVDAAP
                     PGPIRVLSMCAGEARDLLGVLDGHPRRQDVHGRLVELDPDLAARAREHAPATVDVVIG
                     DAGITDVYEGATPADLVLVCGVFGNVTDADVFNTVQMLPLLCAPDATVIWTRHRRPPD
                     LTPALLEEFDRNGFEAIATTQGEVGTMFGVGMHRFRGLPQPFRPHLRLFTFVGYDALE
                     DACPDCGFSYAVGRADALAWLRSDTRSFVDVFARYDDATARTRPEPDVWSPLEYACHV
                     RDVLLVQHDRVVLALETDEPTFVPMRRDERAGELKYNEQDPAVVADEINTASAAFIAL
                     YEQMNDEQWNRRGVYNYPPPQLRTMEWVAIHTVHELLHHRGDITLTR"
     gene            2362..3399
                     /locus_tag="VFX21_06400"
     CDS             2362..3399
                     /locus_tag="VFX21_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HEX5095621.1"
                     /translation="MTYKVIQWATGLLGKESILGILAHPELELVGCWVYSDDKVGKDV
                     GEICGIDPIGVAATNSIDELCALDADAVVYSPVLASTRDVIQLLQSGKNIVTPVGWIY
                     PADSPGVAAIQAACIEGNVTLHGTGINPGGITERFPLMLSAMCRDIRHVRAEEFSDIR
                     NYPTPMVVREVMLFGKDPETAAKSPMVDILGHGFKQSIDMVAAELGWELDEEKPSIHE
                     WAVATQDMDTPVGVIEKGTVAAQRFTWQGTVNGKPVMTVRVNWLMGDDYLDPPWKLGE
                     QRFENELDADPPVSCTFHGLHPPVIGENPEIGLTGPAMHCVNAIPYVVQAEPGIKTYL
                     DLPLLAGRGVV"
     gene            3396..>3621
                     /locus_tag="VFX21_06405"
     CDS             3396..>3621
                     /locus_tag="VFX21_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095622.1"
                     /translation="MSDVTIRRARSDEAATLSPLQEPSREAIALAGSAEYANRFVCAL
                     LERALAVDVPVFVAEREGRPVGFAQLGEPDD"
BASE COUNT          633 a         1202 c         1190 g          596 t
ORIGIN      
        1 cctgcgcggc atggttgcgg tgccgctggt ttacgacccg tacgactacg agatcgatgc
       61 gaatccgcat ccgatctgga agcggatgcg cgacgaagca ccgctctact acaacgagca
      121 atacgacttc tacgcgctga ctcgcttcga cgacgtcttg cgcgcccatc tcgacgcggc
      181 gacgttcagt tcggcgcgca cgaccgtgct cgaactgatg ggcgaagcgc cggcgggctt
      241 cgaggacttc atgatcttca tggatccgcc cgcgcacacc cgttaccgca agctggtcag
      301 tcgcgccttc acgccgcgcc acatcgccga gctcgagatt cgcattcgtg aactgtgcgc
      361 cgactttctc gacccgctcg tcggtgcggg cggcttcgat tacgtcgccg acttcggcgc
      421 gcgcctcccc gtcctggtga tctccgcgtt gctcggtgcg ccgcgcgaag acaccaagca
      481 actgcaggag tggtcggacg cgacgctgca catcgacccg ggcggcatga tgggcgacgc
      541 ggtcgcacag atcaaccgcc agattcacga ctactggcag gcgcacatcg actaccgccg
      601 caagcatccg caggacgaca tcatgggcga gctcatgacg gcggaactcg aaactgccga
      661 cggcggagcg cgacacctga ccgacagcga attgcacggc ttctacggcc tgctgtcggg
      721 cgcgggaaac gaaaccgtcg cgcgcttcct cggctgggca gcgaccgggc tcgaccagtt
      781 cccggccgaa cgggccaagc tcgcggccga cccggagttg attccgaacg cggtcgagga
      841 aatcctgcgc tgggaagcac cgtcggcgat tcagggccga tgggtgaccg aggacgtgcc
      901 gctccacggg tcggtcttgc cgaagaacac caaggtcgca ctcctcaccg gtgccgcgaa
      961 tcgcgacgag cgcgtcttcg agaagcctga cgaaattcag gtcgaacgcg acatcagccg
     1021 ccacgtcgcg ttcggttacg gcattcactt ctgcctcggc gccgcgttgg cccggctcga
     1081 aggacgcatc gcgctggagg agaccttcaa gcggttcccg acatggaccg tcgatcgcga
     1141 caactgcgaa atggtgcaca cgagcaccgt gcgcggctac gccaaggtcc cgatttccac
     1201 gtgagcgacg acgtcgccta acgcgtgagt gtgatgtcgc cgcgatgatg taggagttcg
     1261 tggacggtgt ggatcgcaac ccactccatc gtccgcagtt gcggcggggg gtagttgtac
     1321 acgccgcggc ggttccactg ctcgtcgttc atctgctcgt agagggcaat gaacgccgcg
     1381 ctcgctgtgt tgatctcgtc ggcgaccacc gccggatctt gctcgttgta cttcagctct
     1441 ccggcgcgtt cgtcgcgccg catgggcacg aaggtcggct cgtcggtttc gagcgccagt
     1501 accacccggt cgtgttggac gagcagcacg tcccgcacat ggcatgcgta ctccagcggc
     1561 gaccacacgt ccggttccgg ccgcgtacgt gcggtcgcat cgtcatatcg cgcgaacacg
     1621 tccacgaagg aacgcgtgtc ggatcgcagc cacgcgagcg cgtcggcgcg accgacggca
     1681 tagctgaacc cgcagtccgg acatgcgtcc tcgagtgcgt cgtagccgac gaacgtgaac
     1741 aagcgcaggt gcgggcggaa cggttgcggg agcccgcgga accggtgcat gccgaccccg
     1801 aacatcgtcc ctacttcgcc ttgtgtggtt gcgattgctt cgaacccgtt gcggtcgaac
     1861 tcctcgagga gtgccggggt caggtcaggc ggccgccggt gccgcgtcca gatgacggtt
     1921 gcatccggcg cgcacagcaa cgggagcatc tgcaccgtgt tgaacacgtc ggcgtcggtc
     1981 acgttgccga atacgccgca gacgagcacg agatcggcag gggttgcgcc ctcgtacacg
     2041 tcggtgatcc cggcgtcacc gatcacgacg tccaccgtcg ccggcgcgtg ctcccgcgcc
     2101 cgcgcggcga ggtcggggtc gagctcgacg agccggccgt gcacgtcctg ccggcggggg
     2161 tgaccgtcga gcacgccgag cagatcgcga gcctcgcccg cgcacatgct caggactcgg
     2221 atcgggccgg gtggcgcggc atcgacggcc tcggcgatgt actgctgcac gacccgcagg
     2281 cgctgggaca acggtgagtc cgagctgtcg tacgcgcggt gccactcgtg ccagtcccgg
     2341 accatgagcg aagataccgc catgacgtac aaagtgatcc agtgggcgac cggcctcctc
     2401 ggcaaggagt cgattctcgg catcctcgcc caccccgagc tcgaactggt cggctgttgg
     2461 gtgtacagcg acgacaaggt cggcaaagac gtcggggaga tctgtggcat cgacccgatc
     2521 ggtgttgccg cgacgaacag catcgacgaa ctgtgcgcgc tcgacgccga cgcggtcgtg
     2581 tactcgccgg tgctcgcgag tacgcgtgac gtcattcagc tcttgcagtc gggcaagaac
     2641 atcgtgacgc ccgtcggctg gatttatccc gccgactcgc ccggcgtcgc tgcgatccaa
     2701 gcggcgtgca tcgaaggcaa cgtgacgctg cacggtaccg ggatcaaccc gggcggcatc
     2761 accgagcgtt tcccgctgat gttgagcgca atgtgccgcg acattcgcca cgtgcgtgcc
     2821 gaagagttct ccgacatccg caactacccg acaccgatgg tcgtgcgcga agtgatgctg
     2881 ttcggcaagg accccgagac tgcggccaag agcccgatgg tcgacattct cggccacggt
     2941 ttcaagcagt cgatcgacat ggtcgctgcc gaactgggct gggaactcga cgaagagaag
     3001 ccgtcgatcc acgagtgggc ggtcgcgacc caggacatgg acacgccggt cggcgtgatc
     3061 gagaagggga ctgttgccgc gcaacgcttc acgtggcaag gcaccgtcaa cggcaagccc
     3121 gtgatgaccg tgcgcgtgaa ctggctgatg ggcgacgatt acctcgaccc gccgtggaag
     3181 ctcggcgaac agcgcttcga gaacgagctc gatgccgacc cgcccgtctc gtgcacgttc
     3241 cacggtctgc acccgccggt catcggcgag aatcccgaga tcggcctgac cggtccggcg
     3301 atgcactgcg tgaacgcgat tccgtacgtc gtgcaagccg agcccggcat caagacgtac
     3361 ctcgacctgc cgttactcgc cggccgcggc gtcgtttgag cgacgtcacg attcgcaggg
     3421 cgcgctccga cgaagccgcg accctctcgc cgcttcagga gccatcgcgc gaagcgatcg
     3481 cgctcgccgg cagcgccgaa tacgcaaacc ggttcgtgtg cgcgctgctc gaacgggcac
     3541 tggcggtcga cgtgccggtg ttcgtcgccg agcgcgaagg ccggccggtg gggttcgcgc
     3601 agctcggcga acccgacgat t
//
LOCUS       DASUGA010000325         5532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1184355, whole genome shotgun sequence.
ACCESSION   DASUGA010000325 DASUGA010000000
VERSION     DASUGA010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184355"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="VFX21_06410"
     CDS             <1..951
                     /locus_tag="VFX21_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5095623.1"
                     /translation="STPVTLALGHLLAGAVAVEDEAVAAGRDHLATARLLAAPLLDGR
                     VGAALATARADVAIAERDLDAATAAVDEGIRKVAYTGDDEALAHLCLLGMRVEAQRDA
                     DALGRASERSRTRRAGRIRGYERHIDRVLAAAPAGVDRPELRAIDLTRLAECARLDDA
                     NDPAAWRASADAWDAVQLPRWAAYAKTRLAEALVATGAPSADVELAFSRAVDAAHAID
                     SRRALSDVARIAQRAGVSLPETVPVTGVPEPALDIGGLTPREREVLDLVVEGATNKQI
                     ANRLFISEKTASVHVSRILTKLGATSRQEAAALARRARQR"
     gene            complement(953..1024)
                     /locus_tag="VFX21_06415"
     tRNA            complement(953..1024)
                     /locus_tag="VFX21_06415"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(989..991),aa:Arg,seq:ccg)
     gene            complement(1142..1819)
                     /locus_tag="VFX21_06420"
     CDS             complement(1142..1819)
                     /locus_tag="VFX21_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095624.1"
                     /translation="MNSLDDLELELRKLPGVRAAGFDELDDMLLVQLHVGDERTEQPV
                     PVSASRIAARHSDRPVAVEVVRWRNPSNVLQPAAEAVDVDVAVDIDGDEIDVALTIES
                     EEATRRPRLLAVLAFPDTDELEVHLILEGRRTIGRAAASRGLVGAVEATIDAVRGLGA
                     PIEPRARWARALDDTGDERVLVAVALDHDDEAATINYGLAAGASPIDAAARSTLDALN
                     RKLSRVL"
     gene            complement(1816..2637)
                     /locus_tag="VFX21_06425"
     CDS             complement(1816..2637)
                     /locus_tag="VFX21_06425"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5095625.1"
                     /translation="MGLVTDDLAAGVATTESGIALIYRALDALVEQFELDDAAVVLEE
                     PGLGRQVFCAGRRPLGPDDEELLTSSPGLYTQPALEDHGFDRSLMLSLCVLALRLDVL
                     RYDAWHDPLTGLYDRRSFDRLLEMAISRSNRYGWPFTLVILDLDHFKQINDREGHQAG
                     DASLRELGERFRRALRFGDNASRIGGDEFAMILPNTEPDLVPTLLDRVMNAPGFERDC
                     PSFSFGVAQCPQEATEFDELVRLADTRLYEAKEHDAPAMTHIEIVHEQDARLERG"
     gene            2917..4557
                     /gene="argS"
                     /locus_tag="VFX21_06430"
     CDS             2917..4557
                     /gene="argS"
                     /locus_tag="VFX21_06430"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX5095626.1"
                     /translation="MIRETLHSAIQNALSAAGYPEPPGGIVLTVPKQREHGDVSSPVA
                     LALAKTVGAKPRDVAERLRQILEDAAIAHVERVEIAGPGHLNFFLAPSWLHDVLRTVV
                     DAGDRYGTDDAFAGRRINLEFVSVNPTGPLHAGGGRWVAIGDAIANLLAARGAEVHRE
                     YYLNDAGNQLRTFGDSLYARYRGEAPPEDGYQGDYLIDMAAQLHAELGDDVTPDDACE
                     WGYHKIVDGLKDDLGRIGVHFDTWFSERTLHERGDVAEVLRILDERGHTYESEGAKWF
                     RATAFGDQRDRVLVKSDGSTTYLCNDFAYHRDKFARGWTHLIDIWGADHHGQVKSLHA
                     GLEALGFPAGEPEILLGQFVKLVRDGKEVRLSKRTGNIIALADILDETDADVARMVFL
                     LQGMETPQTFDLDVVTAQSMDNPVYYVQMAHARIASIGRKADAAGVTRIPLADADLSL
                     LTHEREEELLRTLEGYPDVIAEAAEQRAPQKVTTWVRDFARDFHGFYHDCRVLTDDEP
                     LTQARLWLTEACRVGLANALGVLGVHAPDEMTRLDEDA"
     gene            4554..>5532
                     /gene="lysA"
                     /locus_tag="VFX21_06435"
     CDS             4554..>5532
                     /gene="lysA"
                     /locus_tag="VFX21_06435"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX5095627.1"
                     /translation="MTDPFDISLLGRTATVDADGVVAIGGVSLVALAEEFGTPLFVYD
                     ESELRGRCREYHAAFGAGAAYASKAFLCTAMARVVDEERLGLDVATGGELFVALNAGF
                     PATRIVFHGNNKSDDELRAALEAGVGRIAVDSFDELDRIERLVASGCPQPSLHVRVTP
                     GVEAHTHEFIETGTDDSKFGFSLATGDALAAVRRVVASDAMQFAGIHCHIGSQVLRLD
                     SFGRAVEKMVGLVRSIEEATGALVEELNVGGGLGVRYLPEDDPPTIAAYHRTIDEAFS
                     KALADAGVRSRPALMVEPGRSIAAPAALTLYRVGTVKTIDGVGTYVAVDG"
BASE COUNT          843 a         1942 c         1910 g          837 t
ORIGIN      
        1 agcacgccgg tcacgctcgc gctcggtcac ctgctggcgg gcgcggtcgc ggtcgaggac
       61 gaagcggtcg ccgccggccg tgaccacctg gcgaccgcgc gcctgctggc ggcgccgctg
      121 ctcgacgggc gcgtcggcgc ggcacttgcc accgcgcgcg ccgacgtcgc gatcgccgag
      181 cgggatctcg acgccgcgac cgcggcggtc gacgaaggca tccgcaaggt cgcgtacacc
      241 ggtgacgacg aggcgctcgc ccatctgtgc ttgctcggga tgcgcgtcga ggcgcagcgc
      301 gacgccgacg cgttgggccg cgcgtcggag cggtcacgta cgcgccgcgc cggccggatc
      361 cggggctacg agcgccacat cgaccgcgtc ctcgcggcgg caccggccgg tgtcgaccgg
      421 cccgagctgc gcgcgatcga tctcacgcgc cttgccgaat gcgctcgtct cgacgacgcc
      481 aacgaccccg ccgcgtggcg cgcgtccgcg gacgcgtggg acgccgtgca actcccgcgc
      541 tgggccgcgt acgcgaagac ccgcctcgca gaggcattgg tcgcgaccgg cgcgccgagt
      601 gccgacgtcg agctcgcgtt ctcccgcgcg gtcgatgctg cgcacgcgat cgacagccgc
      661 cgcgcactca gcgacgtggc ccgcatcgcg cagcgcgccg gggtgtcgtt gccggagacg
      721 gtgccggtga ccggcgtgcc cgagcccgcg ctcgacatcg gcggcctgac gccgcgcgaa
      781 cgagaagtgc tcgacctcgt ggtcgaaggc gcgaccaaca agcagatcgc gaaccgcctg
      841 ttcatcagcg agaagaccgc aagcgtccac gtctcgcgca tcctcaccaa gctcggcgca
      901 accagccgcc aagaagccgc ggcactggcc cgccgcgccc gccagcggta agcgccctcg
      961 gcaggattcg aacctgcgca catggctccg gaggccactg ctctatcccc tgagctacga
     1021 gggcaagggc ggacgagcgt accaattggc tccgaccatc ggtcgatgaa cgacatccga
     1081 ggtgggtgaa cgaccaatgg tcgcggaacg ctgcccgact gcgttgctcg gaatgcgcgc
     1141 gctagagaac gcgcgacagc ttgcggttga gcgcgtcgag cgtcgaacgg gcggcggcgt
     1201 cgatcgggct ggcaccggcc gcgaggccgt agttgatggt cgcggcttcg tcgtcgtggt
     1261 cgagcgcgac cgcgacgagc actcgctcgt cgccggtgtc gtcgagcgcc cgcgcccaac
     1321 gggcgcgcgg ctcgatcggt gcgcccaggc cccgcaccgc gtcgatcgtc gcctcgaccg
     1381 cgccgaccag accgcgtgac gcggcggccc ggccgatcgt gcggcggcct tcgaggatca
     1441 ggtgcacttc gagctcgtcg gtgtcgggaa acgccaacac ggccagcagg cgcggccgcc
     1501 gggtggcctc ctcgctctcg atcgtaagcg ccacgtcgat ctcgtcgccg tcgatgtcga
     1561 ccgcgacatc gacgtcgacg gcctctgccg cgggctgcag gacgttgctc ggattccgcc
     1621 agcgcacgac ttcgacggca accggtcgat ccgagtgccg cgccgcgatg cgggatgcgc
     1681 tcaccggcac cggctgctcg gtgcgctcgt cgccgacgtg cagctgcacg agcagcatgt
     1741 cgtcgagctc gtcgaagccg gcggcgcgca cgcccgggag cttgcgcagc tcgagctcga
     1801 ggtcgtccag tgagttcatc cccgctccaa cctcgcatcc tgctcgtgga caatttcgat
     1861 gtgggtcatg gccggagcgt cgtgctcctt ggcctcgtac aaccgggtgt cggcgagccg
     1921 caccagctcg tcgaactcgg tcgcttcctg cgggcactgc gcgacaccga aggagaagct
     1981 cgggcagtcg cgctcgaagc ccggcgcgtt catcacgcgg tcgagcagcg tcggtaccag
     2041 gtcgggctcg gtgttgggca ggatcatcgc gaactcgtcg ccgccgatac gtgacgcgtt
     2101 gtcgccgaag cgcaacgcgc ggcggaagcg ttcgccgagc tcgcgcagcg acgcgtcgcc
     2161 ggcttggtga ccttcgcggt cgttgatctg cttgaagtgg tcgaggtcga ggatgacgag
     2221 cgtgaacggc cagccgtagc ggttgctgcg cgagatcgcc atctcgagca gacggtcgaa
     2281 gctgcggcgg tcgtacaggc ccgtgagcgg gtcgtgccac gcgtcgtagc gcagtacgtc
     2341 gagccgcagc gcgagcacgc acaacgacag catcaacgag cggtcgaagc cgtggtcttc
     2401 gagtgcgggc tgggtgtaca gccccggcga cgacgtgagc agttcctcgt cgtcgggtcc
     2461 gaggggccgg cggcccgcgc agaacacctg gcggccgagc ccgggttctt cgaggaccac
     2521 cgcggcgtcg tcgagctcga actgttccac gagcgcgtcg agcgcgcgat agatcagtgc
     2581 gatccccgat tccgtggtcg ccactcccgc ggcgaggtcg tcggttacga gacccatccc
     2641 ccggctcccg atgtcacgca cggtccttgt gtctggttcg tccccgctcg tgcgtgcgcc
     2701 cgggaggact gccgccaagc ctcgaccggc gccgagctcc tgcggcattg cgaccctccc
     2761 tggtgcagct cagcgcggcc ccccaccact tggcctgctg attccatggc tgcttgcccc
     2821 tacccctgta agtgatcgcg aaggatagtg gtctgcgcgc gtcctgtgaa atgccgaatg
     2881 ccgaatcttg gtcaccgtgt accctttgtt ggctaaatga tcagagaaac cctccactcg
     2941 gccatccaaa acgcactctc cgcggccgga tacccggagc cgccgggggg catcgtcctc
     3001 accgtgccca agcagcggga gcacggcgac gtctcctcgc cggtggcgct cgcgctggcc
     3061 aagacggtgg gggccaagcc ccgcgatgtc gccgagcgtc tccgccagat cctggaagat
     3121 gctgccatcg cgcacgtcga gcgggtcgag atcgcgggtc ccggccatct caatttcttc
     3181 ctggccccga gttggttgca cgacgtgttg cggacggtcg tggacgccgg cgaccgctac
     3241 ggcaccgacg acgcgttcgc gggccggcgc atcaacctcg agttcgtctc ggtgaacccg
     3301 accggtccgc tgcacgcggg cggcgggcgg tgggtcgcga tcggcgacgc gatcgcgaac
     3361 ctgctcgcgg cgcgcggcgc ggaggtgcac cgcgagtact acctgaacga cgcgggcaac
     3421 cagctgcgga cgttcggtga ctcgttgtac gcgcgctacc gcggcgaggc gccgcccgag
     3481 gacggctacc agggcgacta cttgatcgac atggccgcgc agctgcacgc cgagctcggc
     3541 gacgacgtca cgcccgacga cgcgtgcgaa tggggctacc acaagatcgt cgacggcttg
     3601 aaggacgacc tgggtcgcat cggcgtgcac ttcgacacgt ggttctcgga gcgcacgctg
     3661 cacgagcgcg gcgacgtcgc cgaggtgctc cggatcctcg acgagcgcgg tcacacctac
     3721 gagagcgaag gcgccaagtg gttccgcgcg accgctttcg gtgaccagcg cgatcgcgtc
     3781 ctcgtgaagt ccgacggcag cacgacgtat ttgtgcaacg acttcgcgta ccaccgtgac
     3841 aagttcgcgc gcggctggac gcatctcatc gacatctggg gcgcggacca ccacggccag
     3901 gtgaagtcgt tgcacgcggg tctcgaagcg ctcgggttcc ccgccggaga accggagatc
     3961 ctgctcggac agttcgtgaa gctggtgcgc gacggcaaag aggtccgcct ctcgaagcgc
     4021 acgggcaaca tcatcgccct cgccgacatc ctcgacgaga ccgatgccga cgtcgcgcgc
     4081 atggtcttcc tgctgcaggg catggagacg ccgcagacgt tcgacctcga cgtcgtcacc
     4141 gcgcagtcga tggacaaccc cgtgtactac gtgcagatgg cgcacgcgcg catcgcgtcg
     4201 atcggtcgca aggccgacgc ggccggggtc acgcgtatcc cgctcgccga tgccgacctc
     4261 tcgttgctca ctcacgagcg cgaggaggag ttgctgcgca cgctcgaggg ctaccccgat
     4321 gtcatcgcgg aagcggccga gcagcgcgcg ccgcagaagg tcaccacgtg ggtgcgtgac
     4381 ttcgcacgcg acttccacgg cttctaccac gactgtcgtg tgctcaccga cgacgaaccg
     4441 ttgacgcaag cccgcctgtg gctcaccgaa gcctgccggg tgggactcgc gaacgcgctg
     4501 ggcgtcctcg gcgtgcacgc ccccgacgag atgacccgcc tggacgaaga cgcgtgaccg
     4561 acccgttcga catctcgttg ctcggtcgca ccgccaccgt cgacgccgac ggtgtcgtcg
     4621 cgatcggcgg cgtcagcctc gtcgcgttgg ccgaggagtt cggcacaccg ctgttcgtgt
     4681 acgacgaatc ggagctgcgc ggccgctgcc gtgaatatca cgccgcgttc ggcgcgggcg
     4741 ccgcctacgc gagcaaagcg ttcctgtgca ccgcgatggc gcgtgtcgtc gacgaggaga
     4801 ggctcgggct cgatgtcgcg accggtgggg agctgttcgt cgcgctcaac gccggctttc
     4861 cggcgacacg catcgtgttc cacggcaaca acaagtccga cgacgagcta cgggctgcgc
     4921 tcgaagccgg cgtcggccgc atcgcggtcg actccttcga cgagctcgac cgcatagagc
     4981 ggctcgtcgc gtcgggctgt ccgcagccgt ccctgcacgt acgcgtcacg ccgggcgtcg
     5041 aggcacatac gcacgagttc atcgagacgg gcaccgacga ctcgaagttc ggcttctcgc
     5101 tcgcgaccgg cgacgcactg gccgccgtgc ggcgcgtcgt cgcgtccgac gcgatgcagt
     5161 tcgccggcat ccactgccac atcggttcgc aagtgctgcg gctcgactcg ttcgggcgcg
     5221 ccgtcgagaa gatggtgggc ctcgtacgct cgatcgagga ggcaaccggt gctctcgtcg
     5281 aggaactgaa cgtcggcggc gggctcggag tgcgctacct ccccgaggac gacccgccga
     5341 cgatcgcggc gtatcaccgc acgatcgacg aagcgttctc caaagcactc gccgacgcgg
     5401 gtgtgcgctc ccggccggcg ctgatggtcg aaccgggacg atcgatcgcg gcgcccgcgg
     5461 cactcacgtt gtatcgcgtc ggcacggtga agacgatcga cggtgtcggc acctacgtcg
     5521 ccgtcgacgg cg
//
LOCUS       DASUGA010000326         5692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_424401, whole genome shotgun sequence.
ACCESSION   DASUGA010000326 DASUGA010000000
VERSION     DASUGA010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424401"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VFX21_06440"
     CDS             <1..927
                     /locus_tag="VFX21_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095628.1"
                     /translation="ATVRRGDKQWAVRMSGSRPDDKLQQQVGPYRIEVVEPFHRIRII
                     CDADDHGLGFDLTYSSDYGPVSEPQHIRRQGDRILLDASRFAGVGTWSGELRFDGETV
                     TVTPDRFTATRDRSWGIRPVGEPEPPGKPNDFSGMWWLWIPLKFDDFMLHVILEEDMH
                     GNRNTNFAIKQYPASTGKPIEQLGWPLPEITYKSGTRWPTGANIDLTARDGKVSTLSI
                     EPIVGIPLNVGCGYGADPEWSHGLWKGDDWIEGSTYDHNDPEVTGRAGFSIADHIARA
                     TFEGQDGWGIFEHAAVGPHAPSGFKDFLDTAP"
     gene            complement(924..1856)
                     /locus_tag="VFX21_06445"
     CDS             complement(924..1856)
                     /locus_tag="VFX21_06445"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5095629.1"
                     /translation="MNADTGAVPQRLAELSARIGRCPDLDTLLSTALGGIAELFGYGH
                     SLLLLLDESNTRCYTIASHGYETQGVGSEVNVGEGVIGMVAARAEPVRVGNLGQMLAY
                     ARTVRRAYEASGEEVPGQEIPLPGLANAESLVAVPAMVLGQLVGVLAIESPERLAFGP
                     GDEALLTVVATLLASAIEIDAAEERCAVPAVGAPSRPAADTSTPVTRVRFFAIDGSTF
                     LDGDYLIKGVAGRVLWALLRQYVDAGRVEFTNKEVRLDPTLELPDFRDNLESRLILLK
                     RRLVERDAPIRIEKTGRGRFRIDVDTQLHLEDAG"
     gene            complement(1853..2287)
                     /locus_tag="VFX21_06450"
     CDS             complement(1853..2287)
                     /locus_tag="VFX21_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010153238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5095630.1"
                     /translation="MTATDEPISLAPIMNCFEGLVPAVIATASSDGTPNVTHLSKVHY
                     VDDDHVALSNQFFSKTMRNLAENPRASVVLVDPTAYGVWKLELRYERTERRGPVFDKM
                     HRDIELVAALTGMAGVFKLKSADIYRVVDLQQVRTAWSTQGQ"
     gene            complement(2284..2757)
                     /locus_tag="VFX21_06455"
     CDS             complement(2284..2757)
                     /locus_tag="VFX21_06455"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5095631.1"
                     /translation="MLIDDEDVALLEGPSSLIVGTVDETGLPDATKAWGVAVQAARER
                     MRVVLSADEERALANARSTGVFALTATNVDTFVSVQVKGRVESVEPESDRDRARREQQ
                     IEGFKRAVNETDGTPIELLGRIEPCAFVTVVATIEEAFDQTPGPTAGRRREPAAS"
     gene            2895..3611
                     /locus_tag="VFX21_06460"
     CDS             2895..3611
                     /locus_tag="VFX21_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5095632.1"
                     /translation="MTAVSVSSTLAVELADLEAVEYDVYRDIHKGIRGELFAVVSEAG
                     RVDPAGQEGVEAVGAHWRSIVELLISHAEHEDGYAQPLIEAHLPELAAKIAYDHPRLE
                     QQMATLEVVADRAANAVGEARRIAVHRLYLGLASFTSDYLAHQAVEELDVMPGLAKVI
                     GVPELIALNQEIVASIPPEQMVKTLPLMMQQMNVDDRTELLFGMKMGAPPEVFAGLSG
                     VVRDALAPEDWDPVAARLAI"
     gene            complement(3662..4522)
                     /locus_tag="VFX21_06465"
     CDS             complement(3662..4522)
                     /locus_tag="VFX21_06465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX5095633.1"
                     /translation="MRIGVVFPQTEIGADVGAVRAYAERVTELGYLHILAYDHVLGAD
                     PEVHTPWRGPYDVHTTFHEPFVLFGYLAAVTSLELVTGIIILPQRQTALVAKQAAEVD
                     LVTRGNFRLGVGIGWNAVEYEALGKDFGDRGKRMERQIPLLRRLWTEESVTSDDGDER
                     VTAAGLAPLPVQRPIPIWIGGSSDPAYRRMGRLADGWFPQVAPGEALDHAREVVGGAA
                     RAAGRDPATIGMEGRVSWTADGLDKLVRQVGKWKEAGATHVTINTMRADLGDVHGHLS
                     VLEQVAAALL"
     gene            4545..5372
                     /locus_tag="VFX21_06470"
     CDS             4545..5372
                     /locus_tag="VFX21_06470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX5095634.1"
                     /translation="MTLRLGIHLPQYGRAAGPDAITRVARAAEDLGFEDVWISDHIVH
                     PAAQSYPSPFLYDPLVTLTWAAATTTRVGLGTSVLVLPHHHPLALANALASLDALSGG
                     RVTVAAGTGWSEAEFDALGQQFARRGARTDEIIRILRACWTEDPVDFAGEFYAVDDIR
                     VLPQPAHPIPIWIGGSGERAYKRAAALGDGFHAIGLHEDDIGPVVERIRRDHPDPSFT
                     FSLRTGWDPQGMEPDQIKRERDAFEAAGVQHIVSAPWRQTADEWIRSMELLVAIVDE"
     gene            complement(5510..>5692)
                     /locus_tag="VFX21_06475"
     CDS             complement(5510..>5692)
                     /locus_tag="VFX21_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5095635.1"
                     /translation="GYALDAASEARMREFLVDNAQDKHGSHTYTWADTGLDMGAVRER
                     AKKYQDYFDVTSESFA"
BASE COUNT          914 a         1977 c         1894 g          907 t
ORIGIN      
        1 gcgaccgtgc gccgcggcga caagcaatgg gcggtccgca tgtcgggatc gcgacccgac
       61 gacaagctgc aacaacaggt cggtccgtat cgcatcgagg tcgtcgagcc gttccaccgg
      121 atccgcatca tctgcgatgc cgacgaccac ggtctcgggt tcgacctcac ctactcctcg
      181 gattacgggc cggtctcgga gccccagcac atccgccggc agggcgaccg catcctgctc
      241 gacgcgtcgc ggttcgcggg tgtcggcacg tggagcggcg agctgcgctt cgacggcgag
      301 acggtcacgg tcacacccga ccgtttcacg gcgacgcgcg accgttcgtg gggcatccgt
      361 ccggtcggcg aaccggagcc gccgggcaag ccgaacgact tcagcggtat gtggtggttg
      421 tggatcccgc tgaagttcga cgacttcatg ttgcacgtca tcctcgaaga ggacatgcac
      481 gggaaccgca acacgaactt cgcgatcaag cagtatcccg cgtcgaccgg caagccgatc
      541 gaacaactcg gctggccgct gccggagatc acctacaagt cggggacgcg ctggccgacc
      601 ggcgcgaaca tcgatctcac cgcgcgcgac ggcaaggtgt cgacactgag catcgaaccg
      661 atcgtcggca tcccgctgaa cgtcggctgc ggctatggcg ccgacccgga gtggagtcac
      721 ggcctgtgga agggcgacga ctggatcgaa ggatcgacgt acgaccacaa cgatcccgag
      781 gtcaccggcc gcgccggctt ctcgatcgcg gaccacatcg cccgcgcgac attcgaaggg
      841 caagacggat ggggcatctt cgagcacgcc gccgtcggcc cccacgcccc cagcggcttc
      901 aaggactttc tagatactgc gccctagccg gcgtcttcga ggtgcaactg ggtgtcgacg
      961 tcgatgcgga agcgaccgcg gccggtcttc tcgatgcgga tcggcgcgtc gcgctcgacg
     1021 agccggcgct tgagcaggat cagccggctc tcgaggttgt cgcggaagtc gggaagctcg
     1081 agcgtcgggt cgagacgcac ctctttgttc gtgaactcga cgcggccggc atcgacgtac
     1141 tggcggagta acgcccacaa cactcggccc gcgacaccct tgatcaagta gtcgccgtcg
     1201 aggaacgtgc tgccgtcgat cgcgaagaag cggacgcgcg tgaccggtgt cgacgtgtcg
     1261 gccgccggcc gagaaggcgc gcccaccgcg ggcaccgcac agcgctcttc ggccgcgtcg
     1321 atctcgatcg cactcgcgag caacgtcgcg acgacggtga gcaacgcctc gtcgccgggc
     1381 ccgaacgcga gccgttcggg gctctcgatc gcgagcacac cgacgagctg accgagcacc
     1441 atggccggca ccgcgacgag gctctccgcg ttcgcgaggc cgggcaacgg gatctcctgc
     1501 ccgggcactt cctcgccact cgcctcgtac gcccgccgca cggtgcgcgc gtaggccagc
     1561 atctgcccca ggttgccgac gcgcaccggc tcggcgcgcg ccgcaaccat cccgatgacg
     1621 ccttcgccga cgttcacctc ggacccgacg ccctgggtct cgtagccgtg actcgcgatc
     1681 gtgtagcagc gggtgttgct ctcgtcgagc agcaacagca gcgagtgccc gtagccgaag
     1741 agctctgcga tcccacccag cgcggtgctg agcagcgtgt cgagatcggg acagcgaccg
     1801 atgcgcgcgc tcagctccgc cagacgctgc ggcaccgcgc ccgtgtcggc attcattgcc
     1861 cttgtgtgct ccacgccgtc cggacttgct ggaggtcgac gacgcggtag atgtcggcgc
     1921 tcttgagctt gaagacgccg gccatgccgg tgagcgcggc gacgagctcg atatcacggt
     1981 gcatcttgtc gaacaccggt ccgcgccgtt cggtgcgctc gtagcgcagt tcgagcttcc
     2041 agacgccgta tgcggtcggg tcgacgagca ccaccgacgc gcgtgggttt tcggcgagat
     2101 tccgcatcgt cttcgagaag aactggttcg acagcgccac gtggtcgtcg tcgacgtagt
     2161 ggaccttcga caagtgcgtg acgttcggcg tgccgtcgct cgacgcggtt gcgatgaccg
     2221 cgggaacgag tccttcgaag cagttcatga tcggcgcgag cgagatgggc tcgtcggtgg
     2281 cggtcatgac gcagccggct cgcgtcggcg acctgcagtc gggcccggtg tctggtcgaa
     2341 cgcctcttcg atggtggcaa cgacggtgac gaacgcgcac ggttcgatac gcccgagcag
     2401 ttcgatcggt gtgccgtccg tctcgttgac cgcgcgcttg aagccttcga tttgttgctc
     2461 gcggcgagcc cggtcccggt cggactccgg ttccaccgac tcgacgcgcc ctttcacctg
     2521 caccgagacg aacgtgtcga cgttggtcgc cgtcaatgcg aacacgccgg tcgagcgcgc
     2581 gttcgccaac gcacgttcct cgtcggccga gaggacgacc cgcatgcgct cgcgagcggc
     2641 ctgtaccgcg acgccccacg ccttggtcgc gtcgggcagc cccgtctcgt cgaccgtccc
     2701 gacgatgagc gaggacggcc cttcgagcag cgccacgtcc tcgtcgtcga tcagcacctg
     2761 cgcactccgg agtccactta cggattactt agcgttgctt agccttcgct cacgcgcctt
     2821 ttgggagcgg gagcgcccgc acttcttcag gatcgtagga gacaagaccc gatctgatcc
     2881 tgaaggagtc accgatgact gcagtgagcg tttcctccac cctcgccgtc gaactggccg
     2941 acctcgaagc cgtcgagtac gacgtctacc gggacatcca caagggcatc cgcggtgagc
     3001 tgttcgccgt ggtctcggag gcgggccggg tcgaccccgc cggccaagaa ggcgtggaag
     3061 ccgtcggcgc gcactggcgg tcgatcgtcg agctcctcat ctcgcacgcc gagcacgagg
     3121 acgggtacgc gcaaccgctg atcgaggccc acctgcccga gctggccgcc aagatcgcgt
     3181 acgaccaccc ccgcctcgaa cagcagatgg ccaccctcga agtcgtcgcc gaccgggccg
     3241 cgaacgcggt gggcgaggct cgccgcatcg ccgtgcaccg cctctacctc gggctggcgt
     3301 cgttcacatc cgactacctc gcgcaccagg cggtcgaaga gctcgacgtg atgccgggtc
     3361 tcgcgaaggt catcggtgtg cccgagctga tcgcgctgaa ccaggagatc gtcgcgagca
     3421 tcccgcccga gcaaatggtc aagacgctgc cgctgatgat gcagcagatg aacgtcgacg
     3481 accgcaccga actgctcttc ggcatgaaga tgggcgcgcc gcccgaggtg ttcgccggac
     3541 tgtcgggcgt cgtgcgcgac gcgctggcgc cggaagactg ggacccggtc gccgcccgcc
     3601 tcgccatctg agttcgtcgg gcgtcttaca ggggcggatt ccgcccgtgt aagacgcccg
     3661 actacagcag tgcggcggcg acttgttcca gcacggacag gtggccgtgg acgtcgccga
     3721 gatcggcgcg catcgtgttg atcgtcacgt gcgtcgcgcc ggcctctttc catttgccga
     3781 cttgacgcac gagcttgtcg agaccgtccg cggtccagct cacgcgccct tccatcccga
     3841 tcgtcgccgg atcacgtccg gcggcgcgcg ctgctcctcc gacgacttct ctcgcatggt
     3901 ccaacgcttc gccgggtgcg acttggggga accaaccgtc ggcgagccgg cccatgcgtc
     3961 ggtacgcggg atccgacgag ccgccgatcc agatcgggat cggacgctgc accggcagcg
     4021 gtgcgagccc cgccgcagtg acccgctcgt caccgtcgtc ggaggtcacc gactcttcgg
     4081 tccagagccg gcgcagcagc gggatctgtc gttccatccg cttgccgcga tcgccgaagt
     4141 ccttgccgag cgcttcgtac tcgaccgcgt tccagccgat gcccacaccg agacggaagt
     4201 tgccccgcgt caccaagtcg acctcggccg cctgcttggc cacgagcgcg gtctgccgtt
     4261 gcgggaggat gatgatgccg gtcaccagct cgagcgacgt gaccgcggcg agatagccga
     4321 acaacacgaa cggctcgtgg aacgttgtgt gcacgtcgta cgggccgcgc catggcgtgt
     4381 gcacctcggg gtcggcaccg agcacgtggt cgtaggcgag gatgtgcaga tagcccagct
     4441 cggtgacacg ctcggcgtac gcacgcaccg cgccgacgtc ggcgccgatc tcggtctgcg
     4501 ggaacaccac tccaatgcgc ataccgaagt tctacccttc cgccatgacg ctgcgcctcg
     4561 gcatccacct cccgcagtac ggacgggcgg ccggccccga tgcgatcacc cgcgttgccc
     4621 gcgccgccga ggacctcggg ttcgaggacg tgtggatcag cgaccacatc gtgcacccgg
     4681 ccgcgcagtc gtacccgtcg ccgttcctct acgacccgct cgtcaccttg acgtgggccg
     4741 cggccacgac gacacgcgtc gggctcggga cgagcgtgct cgtcttgccg catcaccatc
     4801 cgctcgcgtt ggcgaacgcg ctcgcgagcc tcgacgcgtt gagcggcgga cgcgtgaccg
     4861 tcgcggccgg gaccgggtgg tcggaggcgg agttcgacgc gctcgggcag caattcgcgc
     4921 ggcggggcgc gcgtacggac gagatcatcc gcatcctgcg ggcgtgctgg accgaggacc
     4981 cggtcgactt cgcgggcgag ttctacgcgg tcgacgacat ccgcgtgctc ccccagcccg
     5041 cgcacccgat tccgatctgg atcggtggca gcggcgaacg cgcgtacaag cgcgcggccg
     5101 cgctcggcga cggcttccac gcgatcggcc tgcacgagga cgacatcggg ccggtcgtcg
     5161 agcgcatccg gcgcgaccat ccggatccgt cgttcacgtt ctcgctgcgc accggctggg
     5221 atccgcaggg gatggagccc gaccagatca agcgcgagcg cgatgcgttc gaggccgccg
     5281 gcgtgcagca catcgtgagc gcgccgtggc gccagaccgc ggacgagtgg atccgctcga
     5341 tggagctgct cgtcgcgatc gtcgacgagt agccgaaccg gccaacttcc aacggcgata
     5401 gctcaacgtc gcacgaggcc gtgccggagc ttgcggaggc actggcggag cggagggcgg
     5461 gccaacaacc cggcgtgcct cacgcgagca cgaccggagc gacaaagagt tatgcgaagc
     5521 tctcgctcgt gacgtcgaag tagtcctggt acttcttcgc acgctcgcgc accgcaccca
     5581 tgtcgaggcc ggtgtcggcc cacgtgtacg tgtgcgaacc gtgcttgtct tgggcgttgt
     5641 cgaccaggaa ctcgcgcatg cgcgcttccg acgcagcgtc gagcgcgtag cc
//
LOCUS       DASUGA010000327         3337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_524519, whole genome shotgun sequence.
ACCESSION   DASUGA010000327 DASUGA010000000
VERSION     DASUGA010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524519"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="VFX21_06480"
     CDS             <1..1060
                     /locus_tag="VFX21_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5095636.1"
                     /translation="DEAFPAFGTTPRDYGTDVYMRMAARFVQRSVHAGQPFFAYLSVF
                     APHEPATPAPADVDRFAGATAPRTPSYDEADVSAMPVYIRDLPQLTPDESAAIDKLYQ
                     RRLESLQAVDRGVANLIATLRSLHALHDTYVVFTSDNGFHLGQHRLPAGKETPYDTDI
                     RVPLAMRGPGIPAHSHVDAMTANTDLAPTFAAMAGAPRDPEWDGRSLLRLAQGDAPPH
                     AGARRAQLVEHWTESPTDRPLPPGIFEPPDVDQSDPRLHPTSMSRPDLLSDHTIYGRL
                     VKIPDYAGVRTRNYLYVEYINGDRELYDVNADPDEMHNLAGTQPELEARLSQLVAALR
                     VCRGAMCRALERSDPAGP"
     gene            1307..2481
                     /locus_tag="VFX21_06485"
                     /pseudo
     CDS             1307..2481
                     /locus_tag="VFX21_06485"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
     gene            complement(2498..>3337)
                     /locus_tag="VFX21_06490"
     CDS             complement(2498..>3337)
                     /locus_tag="VFX21_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095637.1"
                     /translation="CFAGAWAQHVADGNDTSLRLSDDDLDHALAGFLELRDPSGVDGG
                     DDGAHGNAFDRVGAFQDGLEGGARACRDYATNPPEVTEAGFSSYADAEVNGDLPLDEV
                     LPMLETSLDDYWTATYSDYERTPKLTATTGAPSCPSGSDRGVLSDSVLYCAATNTINY
                     STQTLNKAEKTIGDLGAGVFVAAAWSSAVQHQLGVKIGTAQARASAECLTGAWAGGVE
                     NGSAGTRKSRDLTFSPGDLDEVVATFVATDGSSSVNRGSVFTRVTLFRTGFEHGAKAC
                     FSA"
BASE COUNT          521 a         1219 c         1090 g          507 t
ORIGIN      
        1 cgacgaagcg ttccccgcat tcggcaccac gccgcgcgac tacggcaccg acgtgtacat
       61 gcgcatggcc gcccgcttcg tgcagcggtc ggtgcacgcc ggccagccgt tcttcgccta
      121 cctctcggtg ttcgcgccgc acgagccggc cactcccgcc cccgccgacg tcgaccgctt
      181 cgccggcgcc acggctccgc gcacaccgag ctacgacgag gccgacgtca gcgcgatgcc
      241 ggtgtacatc cgcgatctgc cacagctcac gcccgacgag tccgccgcga tcgacaagct
      301 gtaccagcgc cggctcgagt cgttgcaagc ggtcgaccgc ggtgtcgcga atctcatcgc
      361 cacgcttcga tcactgcacg cgctgcacga cacctacgtc gtgttcacct ccgacaacgg
      421 gttccacctc ggacagcacc ggctccccgc tggcaaggag acgccgtacg acaccgacat
      481 ccgcgtcccg ttggcgatgc gcggtcccgg catcccggcg cacagccacg tcgacgccat
      541 gacggccaac accgacctcg ccccgacctt cgccgcgatg gccggcgcac cgcgcgaccc
      601 cgaatgggac gggcgatcgc tgctccgtct cgcgcagggt gatgcacccc cgcacgccgg
      661 cgcacggcgc gcccaactcg tcgagcactg gaccgagagt cccaccgacc ggccgctccc
      721 gcccggcatc ttcgagccgc ccgacgtgga ccagtccgat cctcgactgc acccgacgtc
      781 gatgtcgcgg ccggacctgc tcagcgatca cacgatctac ggccggctcg tgaagatccc
      841 cgactacgcc ggcgttcgca ctcggaacta tctctacgtc gagtacatca acggcgatcg
      901 cgagctgtac gacgtgaacg ccgatcccga cgagatgcac aacctcgccg gcacgcagcc
      961 ggagctggag gcacggctct cgcagctggt cgctgcgctg cgcgtgtgcc gcggcgcgat
     1021 gtgccgcgcg ttggaacggt cggaccccgc cggtccatga tgaccaggtg gacctggagg
     1081 ttgtagggat cgcgcgcgcg tgagcgacga cgtcgcgcgc atcaccgcgc tcgccgtcgc
     1141 accgcgcgcg gagctgcacg agcggggccg gaccgagcgt cacgaagtgc cgcgccgcgc
     1201 ccatgcagag tggaaaccgc ctccggatcg tcgctcaccg ctcgacatcc tcgccgagag
     1261 caatgcgtca cgcgtccccg agctcgtgcc gatccgatac gggcgcatgg cggcgaaccc
     1321 gtttgcctac ttgcgcggtg cgccggccgt catggccgcc gacctcgccc gcaccccgag
     1381 gacgcggatc tggccccaga tctgcggtga cgggcacctc tcgaacttcg gcgtcttcgg
     1441 gacacccgag cgttcgatga tcttcgacct caacgacttc gacgagaccc tgccgggtcc
     1501 gttcgaatgg gatctcaagc gcttgaccgc gagcatcgtg gtggccgcgc gagcccacgg
     1561 tttcggcaac caggcgggcc tcgacgccgc tcgcgaggcg gtacgcggct acgcgtaccg
     1621 gatcgagcag ctgccgcacg gcccacgctc gacatctggt acgccaagac caccgagacc
     1681 gacgttctcg cactcgccaa ggggcgaagc ctgcaacgcc ggctcgagaa ggacttcgcc
     1741 aaggcccgtc ggcgggactc gatgcaggca gtcgccaaac tcacccggat catcgacgac
     1801 cggccggtga tcgtggatga ccctccgctc gtcgtgcacg accagacatc caacgacgcc
     1861 gcgaccgtgc accacttctg gcgcgagtac cacgccggct tgcgcgggga tcggcgtcat
     1921 ctcgccgacc agtacgagat cgtcgacgtc gctcgcaagg tcgtcggcgt gggcagcgtg
     1981 ggcacgcgct gctacgtcgc gctcctcgca ggacgtggca ccgaggacct cttgatgctc
     2041 caggtgaagg agtcgcaggc ttcggtgctc gagccgtacc tcggccgcag cgagttcgac
     2101 catcatggcg agcgcgtcgt gacggggcag cgactccttc aggcggcgag cgacatcttt
     2161 ctcggctggg tcacgagcgt ggtcggtcac gactacttcg tgcgccagct ccgcgacatg
     2221 aagtactcag ccgacgttgc gacgctgccg ggcagtggct tcttccagta cgcgtcgctg
     2281 tgcggttcca cgctcgctcg cgcgcacgcg aacacgtgcg acccggcata catcgcgggc
     2341 tacatcggac gtggcggtgt gctcgcggac gcgttggtgg agttcgccaa cgcatacgcg
     2401 acgcagaccg agcacgatca tgcggagctc gtcaccgcca tccacgacgg tgacgtcgac
     2461 gcggtcttcg aggcgccgtg accgcggccc gtgcgattca ggcgctgaag caggccttgg
     2521 cgccgtgctc gaagccggtt cggaacagcg tgacgcgagt gaacaccgag ccgcggttca
     2581 ccgacgacga tccgtcggtt gccacgaacg tcgcgaccac ctcgtcgagg tcgccgggcg
     2641 agaacgtgag gtcgcgcgac tttcgggtgc cggcgctgcc gttctcgacg ccaccggccc
     2701 aggccccggt caggcactcg gccgacgcac gcgcctgcgc ggtgccgatc ttcacgccga
     2761 gctggtgctg cacggccgac gaccacgccg cggcgacgaa cacaccggcg ccgaggtctc
     2821 cgatcgtctt ctccgccttg ttgagggtct gggtcgagta attgatcgtg ttcgtcgccg
     2881 cgcagtacag caccgagtcg ctgaggacgc cgcggtccga gccgctcgga cacgacggcg
     2941 cacccgtcgt ggccgtgagc ttcggcgtcc gctcgtagtc ggagtacgtc gcggtccagt
     3001 agtcgtcgag gctcgtctcg agcatcggca agacttcgtc gagtggcaga tcgccgttca
     3061 cctcggcgtc ggcgtagctg ctgaacccgg cttcggtcac ttcgggcgga ttcgtcgcgt
     3121 agtcgcgaca ggcgcgcgcg ccgccttcga gcccgtcctg gaacgcgccg acgcgatcga
     3181 atgcgttccc gtgcgcgccg tcgtcgccgc cgtcgacgcc cgacgggtcg cgcagctcga
     3241 ggaaaccggc cagcgcgtgg tcgaggtcgt catcggagag tcggagcgac gtgtcgttgc
     3301 cgtcggcgac gtgttgcgcc cacgcgccgg caaagca
//
LOCUS       DASUGA010000328         6153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1593441, whole genome shotgun sequence.
ACCESSION   DASUGA010000328 DASUGA010000000
VERSION     DASUGA010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593441"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="VFX21_06495"
     CDS             complement(<1..1123)
                     /locus_tag="VFX21_06495"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX5095638.1"
                     /translation="MAIDVREFHTRTLDPVISEELDIFESTVSEYLDGRVDEDTFRVF
                     RLNHGVYGQRQGGRNQMLRVKVPHGRTTAEQLDMYAYIADTYSRGWGHLTTRQNVQFH
                     FIQLEQSPEILRLLASVGLTTREACGDTVRNVMGCHLAGACPQEVLDITPWAQATTDF
                     LLRHPYGQRLPRKFKINFSGCATDCGQAMFNDVGVVATTRTLDDGTIEAGFRVFIAGG
                     LGANPHPAQALEDFTSREDLMATIYACLRTFDHYGNRDNKIRARMKWLVDTLEGGIDE
                     LRERIFKERKFLRGARDWPGGIPQYVLEHGDAPAGGTPDATVVGATAVQVKLRSSDPY
                     ERWIEANVVRGVAKGTVSAVAYARLGDITSDQWRALASIQ"
     gene            complement(1400..2068)
                     /locus_tag="VFX21_06500"
     CDS             complement(1400..2068)
                     /locus_tag="VFX21_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095639.1"
                     /translation="MRVRLFLAASGAAVAFALAAPAGAIDVPGDGFTVTGEFGVGGTI
                     TITDDDPTPCDGENGSASLSRPIGGSNFIPVVSNVAPLVEGQVWSVTLPIPATDKGNP
                     ATALVPGMTLVLDVGGVCNDLGDQFGFQREDLELTLGEPVTAPATTTTTTDPGATTTT
                     VTAGAVPTTATASTTPTTAGAAAAAELPRTGGGSGVPIGIAFVLVGAAVVVAAGRRPA
                     RRGA"
     gene            2191..3876
                     /locus_tag="VFX21_06505"
     CDS             2191..3876
                     /locus_tag="VFX21_06505"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX5095640.1"
                     /translation="MRMSELFLRTLRDDPADAEVDSHRLLVRAGFIRRIAAGFYSWLP
                     LGRRVLRNVEQIVREEMDRAGAQEVILPIAQPRELWERSGRDALYGPLMFRLEDRKET
                     GFNLTPTSEEVVTTLVAAEYSSYRDLPLNLYQINWKYRDELRPRFGLLRSREFLMKDA
                     YSFDVDLDALRVSYKKMYDAYHRVFERCGLTFRPVEAQSGEIGGDVNHEFMAVAAVGE
                     DDFVWCTSCDYAANVEAARRDTTSWDTTATVDAPPMEKVHTPDMPGIAPLAEFFGIDA
                     DATLKCIAFDVDGELGLALVPGDREVNEYALRAAVAPRSMRLYGDEDFDAHLELPKGY
                     IGPHFGASIVVADPSVAAPKGWYTGANQNDYHVKNAVLGRDFSVDIWAGIVTIVSGDP
                     CPRCGQPLSVDRGIEVGHVFQLGTKYAEALDARYTDEAGEQHAMVMGCYGIGVSRVVA
                     AVVEEHHDEQGFKWPAAIAPYDVHLIVLPGRGETAEKVTAEAERVYAMLQERGTSVLF
                     DDRDASPGVKFADADLLGMPVQLVVGAKGLANGIVERKTRATGEREDVPLGDV"
     gene            3873..4937
                     /locus_tag="VFX21_06510"
     CDS             3873..4937
                     /locus_tag="VFX21_06510"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX5095641.1"
                     /translation="MKLPVMPPVEPMLAKLTRELPPGGPVMFEPKWDGFRCILFRDGD
                     DLDLQSRNQKPLLRYFPELRDPMLSQLPDRIVLDGELVVWNDSGLDFDALQLRQHPAE
                     SRIKKLAAEIPASFVAFDMLALGDESWCERPFRERRAELESIMAKTEPPLYLTPATLD
                     RDEAHDWFTRFEGAGFDGVVAKPLDDTYRPGQRSMLKVKHERTADCVAAGFRVHKDGK
                     GVGSILCGLYDDDGDLHHVGVASGMSAAVRAQLLEEIEPLREHALEGHPWRDWAEAMQ
                     SGTRMPGGPSRWNADKDLSWEPLRIERVVEVAYEGLMNGRFRHNARFRRWRDDRDPDS
                     CTYSQLEAVAPAELREMFGK"
     gene            4950..>6153
                     /locus_tag="VFX21_06515"
     CDS             4950..>6153
                     /locus_tag="VFX21_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5095642.1"
                     /translation="MQPSDRADVGASPFGRAVADFEGIVRDGLELAANLDVADLGEVS
                     PERAAHLEAFALVASFIAADGDIAFEERAAVLDLFPDFATRPPLAKREWKLQPSPTLL
                     TILQAAVVVGEPDPIVLYKFAALELARRVIALDLELRDPELAELSQFEEMIAGVTLAM
                     NEKLHRAGGNSARADAPPDLDAVFAELDALVGLRAVKRQVRDVANLLRIHAIRREEGL
                     PVPDVSHHMVFTGNPGTGKTTVARLLAQIYASLGVVRRGQLVEATRADLVGEYVGQTA
                     VRTTEVVNRALDGMLFIDEAYALARSETRREDFGAEAIDTLVKLMEDHRGSLVVVVAG
                     YPEPMKTFLASNPGLPSRFKRTIHFSDYTPDELVEIFVKLAADAGYEVAADARGRVRQ
                     VFVDLDEDT"
BASE COUNT          939 a         2064 c         2196 g          954 t
ORIGIN      
        1 gctggatcga ggcgagcgcg cgccactggt cgctggtgat gtcgcccagg cgggcgtagg
       61 cgacggcgga gacggtgccc ttggcgacgc cgcgcacgac gttggcctcg atccaccgtt
      121 cgtaggggtc gctcgagcgc agcttcacct gtaccgccgt cgcgccgacg accgtcgcgt
      181 cgggtgtgcc gcccgcgggc gcgtcaccgt gctcgaggac gtactgcggg atgccgcccg
      241 gccagtcgcg ggcaccccgc aggaacttgc gctccttgaa gatgcgttcg cgcagctcgt
      301 cgatgccgcc ttccagggtg tcgacgagcc acttcatgcg ggcgcggatc ttgttgtcgc
      361 ggttgccgta gtgatcgaac gtgcgcaggc acgcgtagat cgtcgccatc aggtcttcgc
      421 gcgacgtgaa gtcttcgagc gcctgcgcgg ggtgcgggtt ggcgccgagg ccgccggcga
      481 tgaacacgcg gaagccggct tcgatcgtgc cgtcgtcgag cgtgcgcgtg gtcgcgacga
      541 cgccgacgtc gttgaacatc gcctggccgc agtcggtcgc gcagccggag aagttgatct
      601 tgaacttgcg cggcagccgc tggccgtagg ggtggcggag caagaagtcg gtggtggcct
      661 gcgcccacgg cgtgatgtcg agcacttcct gggggcaggc gccggcgagg tggcagccca
      721 tcacgttgcg gacggtgtcg ccgcacgctt cacgcgtcgt caggccgacc gacgcgagga
      781 ggcgcaggat ctcgggcgac tgctcgagct ggatgaagtg gaactgcacg ttctggcgcg
      841 tcgtgaggtg gccccacccg cgcgagtacg tgtcggcgat gtacgcgtac atgtcgagct
      901 gttccgcggt cgtgcggccg tgcggcacct tcacgcgcag catctggttg cggccgccct
      961 ggcgttgccc gtacacgccg tggttcagcc ggaagacgcg gaaggtgtcc tcgtcgacac
     1021 ggccgtcgag gtactcggac acggtgctct cgaagatgtc gagctcttcg ctgatcaccg
     1081 ggtcgagcgt gcgagtgtgg aattcgcgga cgtctatcgc catgaggcgg ccctctcggg
     1141 acggggaacg gtcggacggc ggagcggcgg gcgcgggcga gcgcggccgc tcagcgggtg
     1201 gtcgcgcgac acaactcgtc gcgcagctcg tgcggcgtgc ggcccatgaa gatgctcggc
     1261 gcgccgttca cccggagaat cacgccccgg aggctaccgg aatcgggccc taaataccac
     1321 cgtggaggtc gggaatttac ccgacctcca cggtcaacaa tctcgagtgc gttggtggac
     1381 gccggccggc gcccgggtcc taggcgccgc gccgggccgg tcgacggccc gcggcaacga
     1441 cgacagccgc cccgaccagg acaaacgcga taccgatggg tacgccggac ccaccgcccg
     1501 tccggggcag ctcggccgcg gccgcggccc cggctgtcgt cggcgtcgtg ctcgcggttg
     1561 cggtcgtcgg caccgcgccc gcggtgaccg tggtggtcgt cgcgcccggg tcggtggtcg
     1621 tcgtcgtggt cgccggcgcg gtgaccggtt cgccgagggt cagctcgagg tcttcgcgct
     1681 ggaacccgaa ctggtctccg aggtcgttgc agacgccgcc gacgtcgagc accagcgtca
     1741 tgccgggcac gagcgcggtg gccgggttgc ccttgtcggt cgccgggatc ggcaacgtca
     1801 ccgaccacac ctggccctcg acgagcggcg cgacgttcga cacgaccggg atgaagttgc
     1861 tgccgccgat cggccgcgag agcgatgcgc tgccgttctc gccgtcgcag ggcgtcgggt
     1921 cgtcgtcggt gatcgtgatg gtcccgccga cgccgaactc gcccgtgacc gtgaagccgt
     1981 ctccgggaac gtcgatggcg ccggccggag ccgcaagtgc aaaggcgacg gcggcgccgg
     2041 aggccgcgag gaacagtcgg acgcgcatgg ttgccctccc gaaggtgttg tcggtaccgg
     2101 ccggacggta acgacgggcg atcacatgag catcacatcg cggtcgaact ccgcgagtgg
     2161 ctcggggcga tccttgtagc ctcgaactcc atgcgtatgt cggagttgtt cctgcggacg
     2221 ttgcgagacg atcccgccga cgccgaggtc gacagccacc gcctgctcgt gcgggccggc
     2281 ttcatccggc ggatcgcggc gggcttctac tcgtggctgc cgttggggcg gcgggtgctc
     2341 cgcaacgtcg agcagatcgt gcgcgaagag atggaccgcg ccggcgccca ggaagtgatc
     2401 ctgccgatcg cccagccgcg cgagctgtgg gagcgcagcg gccgcgacgc gctctacggg
     2461 ccgttgatgt tccgcctcga agaccgcaag gagaccggct tcaacctcac gccgacgtcg
     2521 gaagaggtcg tcacgacgct ggtcgcggcg gagtactcgt cgtatcgcga cctgccgctc
     2581 aacctgtacc agatcaactg gaagtaccgc gacgagctgc ggccgcgctt cgggctgttg
     2641 cgcagccgcg agttcctgat gaaggacgcc tattccttcg acgtcgacct cgacgcgctg
     2701 cgcgtgagct acaagaagat gtacgacgcc taccaccgcg tcttcgaacg gtgcgggctg
     2761 acgttccggc cggtcgaggc gcagtcgggc gagatcggcg gcgacgtcaa ccacgagttc
     2821 atggcggtcg ccgccgtcgg cgaagacgac ttcgtgtggt gcacgtcgtg cgactacgcg
     2881 gccaacgtcg aagcggctcg gcgcgacacg acgtcgtggg acacgaccgc gacggtcgac
     2941 gcgccaccga tggagaaggt gcacacaccc gacatgcccg gcatcgcgcc gctggccgag
     3001 ttcttcggca tcgacgcgga cgccacgttg aagtgcatcg cgttcgacgt cgacggcgag
     3061 ctcggcctcg cgctggttcc cggcgaccgt gaggtcaacg agtacgcgct gcgcgccgcg
     3121 gtcgcgccca ggtcgatgcg gctctacggc gacgaagact tcgacgcgca tctcgagcta
     3181 ccgaagggat acatcggccc gcacttcggg gcatcgatcg tcgtggccga tccttcggtc
     3241 gcggcgccga aggggtggta cacgggcgcg aaccagaacg actaccacgt caagaacgcg
     3301 gtgctcggcc gcgacttcag cgtcgacatc tgggccggca tcgtcacgat cgtgtccggc
     3361 gacccgtgcc cgcggtgcgg gcagccgctg tcggtcgacc gcggcatcga ggtcggtcac
     3421 gtgttccagc tcggcaccaa gtacgcggag gcgctcgacg cccgttacac cgacgaggcc
     3481 ggcgagcagc acgcaatggt gatgggctgc tacggcatcg gtgtctcacg tgtggtcgcc
     3541 gcggtcgtcg aggagcatca cgacgagcag ggcttcaagt ggccggccgc gatcgcgccc
     3601 tatgacgtcc acctcatcgt gctgcccggc cgcggcgaga ccgcggagaa ggtcaccgcc
     3661 gaagcggaac gcgtctacgc gatgttgcag gagcgcggca cctcggtgtt gttcgacgac
     3721 cgcgacgcga gccccggtgt gaagttcgcc gacgccgacc tcctcgggat gcccgtgcag
     3781 ctcgtcgtcg gtgccaaagg gcttgccaac ggcatcgtcg agcgcaagac gcgcgcgacg
     3841 ggcgagcgcg aagacgtccc gttgggtgac gtgtgaagct cccggtcatg ccgcccgtcg
     3901 agccgatgct cgcgaagctg acgcgagagc tgccgcccgg cggcccggtg atgttcgaac
     3961 cgaagtggga cggcttccgc tgcatcttgt tccgcgacgg cgacgacctc gatctgcaga
     4021 gccgcaacca gaagccgctg ctgcgctact tccccgagct gcgcgacccg atgctctcgc
     4081 agctcccgga ccgcatcgtg ctcgacggcg agctcgtggt gtggaacgac tcgggcctcg
     4141 acttcgacgc gctgcagctc cgccagcacc cggccgagtc gcgcatcaag aagctcgcgg
     4201 ccgagatccc ggcgtcgttc gtcgcgttcg acatgttggc gctcggcgac gagtcgtggt
     4261 gcgaacgccc gttccgcgag cggcgcgcgg agctcgaatc gatcatggcg aagaccgagc
     4321 cgccgctgta tctcacaccc gcgacgctcg accgcgacga agcgcacgac tggttcacgc
     4381 gcttcgaagg cgcgggcttc gacggcgtcg tcgccaagcc actcgacgac acgtaccggc
     4441 ccgggcagcg ttcgatgctg aaggtgaagc acgagcgcac cgccgactgt gtcgccgccg
     4501 gcttccgggt gcacaaggac ggcaagggtg tcgggtcgat tctctgcggt ctctacgacg
     4561 acgacggcga cttgcaccac gtcggcgtcg cgagcggtat gtccgcggcc gtgcgggcgc
     4621 agctgctaga ggagatcgaa ccgctgcgcg agcacgcgct cgagggtcat ccgtggcgcg
     4681 actgggccga ggcgatgcag tccgggacgc gcatgcccgg cgggccgtcg cggtggaacg
     4741 ccgacaagga tctgtcatgg gaaccgttgc gcatcgagcg tgtcgtcgag gtcgcatacg
     4801 aagggctcat gaacggccgg ttccgtcaca acgctcgctt ccggcgctgg cgcgacgacc
     4861 gtgatcccga ttcctgtacg tactcgcagc tcgaagcggt cgcgccggcc gagctgcgcg
     4921 agatgttcgg gaagtaacct ggtgacggta tgcagccaag cgaccgggcg gacgtaggcg
     4981 cgagcccctt cggccgcgcc gtcgccgact tcgaaggcat cgtccgcgac ggcctcgagc
     5041 tcgccgccaa cctcgacgtc gccgacctcg gtgaggtcag ccccgagcga gccgcgcacc
     5101 tcgaagcctt tgcgctcgtc gcgtcgttca tcgcggccga cggcgacatc gcgttcgaag
     5161 aacgggccgc ggtgctcgac ctcttcccgg acttcgccac gcggccgccg ctggccaagc
     5221 gcgagtggaa gctccagccg tcgccgacgt tgctcaccat cctccaggcc gcggtcgtcg
     5281 tgggtgagcc cgatccgatc gtgctctaca agttcgccgc gctggagctg gcccgccgcg
     5341 tgatcgcgct cgacctcgag ctgcgcgatc ccgagctcgc cgagctctca cagttcgagg
     5401 agatgatcgc cggcgtcacg ctcgcgatga acgagaagct gcaccgagcg ggtggcaact
     5461 cggcgcgtgc cgacgcgccg cccgatctgg atgcggtgtt cgcggagctg gacgcactgg
     5521 tcggcctgcg ggcggtcaag cggcaggtgc gcgacgtcgc gaacctgcta cgtatccacg
     5581 cgatccgacg cgaggagggc ctccccgtcc ccgacgtgag tcatcacatg gtcttcaccg
     5641 ggaacccggg caccggcaag acgactgtcg cgcggttgct cgcgcagatc tacgcgtcgc
     5701 tcggcgtcgt gcgccgcggg cagctcgtcg aagcaacacg cgcggatctg gtgggcgaat
     5761 acgtcgggca gaccgcggtg cggacgaccg aagtcgtcaa ccgcgcgctc gatggcatgc
     5821 tgttcatcga cgaggcgtac gcacttgccc gcagcgagac gcgacgcgaa gacttcggcg
     5881 cggaagcgat cgacacgctc gtgaagctga tggaggacca ccgcggctcg ctcgtggttg
     5941 tcgtcgccgg ctacccggag ccgatgaaga cgttcctcgc gtcgaacccg gggttgccga
     6001 gccggttcaa gcgcacgatc cacttctccg actacacgcc cgacgagctc gtcgagatct
     6061 tcgtgaagct ggcggccgac gcgggctacg aggtcgccgc cgacgcgcgc gggcgtgtgc
     6121 ggcaagtgtt cgtggacctc gacgaagaca ccg
//
LOCUS       DASUGA010000329         4273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_284073, whole genome shotgun sequence.
ACCESSION   DASUGA010000329 DASUGA010000000
VERSION     DASUGA010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284073"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..523
                     /locus_tag="VFX21_06520"
     CDS             173..523
                     /locus_tag="VFX21_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020101143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5095643.1"
                     /translation="MSIKVELAEVQAVAAQQMGFAYLLTVSDDATPHLVAITPAFGDG
                     EITCDAGKTSCRNIGERPNVSLLWPPLAREDYSLIVDGNATVDDGTVRIAPTRAVRHR
                     PAPGGGNDCAPVSL"
     gene            complement(513..1364)
                     /locus_tag="VFX21_06525"
     CDS             complement(513..1364)
                     /locus_tag="VFX21_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX5095644.1"
                     /translation="MTLTEPGISEHVARIEADGYTIVNDAIEPDLVTNLRDTIRRLER
                     ELDLRPRDTSAEGHATLRMYNLLAKDPVFQAMPVHGSVLPIVERLLDGGCLLSGMTAI
                     DIGPGEHPQPMHGDDIVMSRHLQRPHAPMFVTSMWALTDFTAANGGTWFVPGSHQFAG
                     TPDAPGALDDVTIEKLEMPAGSVMIFHGSLWHGGGANTTADEWRCGVNVQYCPGFVRQ
                     QQNPYLGIPVEIAETFSDRLLSLLGYRLYKGIMGHIDGASPGAFVFGDRFAETAYKTS
                     EERRAKG"
     gene            1375..2064
                     /locus_tag="VFX21_06530"
     CDS             1375..2064
                     /locus_tag="VFX21_06530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5095645.1"
                     /translation="MADVEPLDNPIWHALTGPQAMFSEGTGLALRFEPEVAVFAALPD
                     QVAADAWDALRALVGNGGAIVARPQLEPPPGWRSPFRLDGFQMIAPDSFGRGAPDDRF
                     VRLTNDDVPEMLALVERTRPGPFAKRTIELGPYYGLRDDAGELVAMSGTRMRLPGYAE
                     LSAVCTDPALRGKGIATALMETVAAGMRSNGEQPMLHVAENNSTAIALYEHLGFVRRS
                     RFTFALVQPPE"
     gene            complement(2033..2542)
                     /locus_tag="VFX21_06535"
     CDS             complement(2033..2542)
                     /locus_tag="VFX21_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX5095646.1"
                     /translation="MHVVLIGLMGSGKSTVGRPLAACLGFAFVDNDVMLERRTGRRAR
                     DIAAADGVDALHQLERAVLADALSDPVPAVITAAAAAPLVDGAEQFAGHHVVYLHGEP
                     STLAPRLVGGGGHRPFVDAGDTLELLRAQYAARDPIYRMLATTIVDAGRPPDAIVEAI
                     IQAVAPTRT"
     gene            2595..3722
                     /locus_tag="VFX21_06540"
     CDS             2595..3722
                     /locus_tag="VFX21_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011601760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEX5095647.1"
                     /translation="MRVPPARVLFPEEDRAEILARIDEALATGQLTLGPLGRDLEAAF
                     AQRHQREHAIAVSSGTSALEIILRAIGVEGREVLVPANTFFATAAAAAHAGARVKFVD
                     CNTDTMAFDLDDVAAQIGPQTAAVIAVHIGGLISPDVPALAELCAQHGVELVEDAAHA
                     HGSALDGHAAGTFGIAGAFSFYPTKVMAGGEGGMIVTNNATIDEEARTYRDQGKGSFL
                     SNFHTRLGANWRMSEPHAAIVHAQLRRLDEFIAARQNLAKRYDAVVESIGLQPFISPP
                     NAHCNYYKYIAFLPEGVDRTDVKQAMRERFDVGLSGEVYDTPLHRQPVFETFADRELP
                     NAEWLCARHICLPLYPSLSDTDADYVIESLATVLAERGVPA"
     gene            3737..>4273
                     /locus_tag="VFX21_06545"
     CDS             3737..>4273
                     /locus_tag="VFX21_06545"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5095648.1"
                     /translation="MQDNLIAVTGGSGFVGSHVVDALLGAGYEVRVIDPKKPYQPEAD
                     WADVDMLDQDAVTDAVKGASAVFHLAAMADVNDIVADPAGSVALNTLGVARVLEAGRR
                     ADTGRVIVSSTVWVYAATHGDVVDETTLFDLETDRHLYVSEKIAAEMFCRDYATLFGR
                     PYTCLRYGIPFGPRMRSDL"
BASE COUNT          717 a         1472 c         1405 g          679 t
ORIGIN      
        1 ctcatggtgt tcggcggccg cttccaagcc gactacttgc acggcgtgcc gaaggtgcag
       61 cacgccgtgc gcggccgaat ctccgcccaa tggcgccaca cctccaagcg cggccgccgc
      121 gacaccaacc ccggctacta cgccccgcga gaattctcga aaggacgctg acgtgtcgat
      181 caaggtcgag ctcgcagaag tacaagcagt cgccgcgcag cagatgggct tcgcctatct
      241 cttgacggtc tccgacgacg cgacaccgca tctcgtcgcg atcacgcccg cgttcggcga
      301 tggcgagatc acctgcgacg cagggaagac gtcgtgccgc aacatcgggg agcgcccgaa
      361 cgtgtcgttg ctctggcctc cgctcgcacg cgaggactat tcgctgatcg tcgacggcaa
      421 cgcaaccgtc gacgacggca ccgtgcgcat cgcaccgacg cgcgcggtgc gccaccgccc
      481 cgcgccgggc ggcgggaacg actgcgcacc cgtcagcctt tagcgcgccg ttcttccgag
      541 gtcttgtacg cggtttcggc gaaacggtca ccgaagacga acgcgcccgg gctcgcgccg
      601 tcgatgtgac ccatgatccc cttgtacagc cggtagccga gcaacgacag caaccgatcg
      661 gaaaaggtct ccgcgatctc gactggaatc ccgagatacg gattctgctg ttggcgaacg
      721 aagcccgggc agtactgcac gttcacaccg caccgccact cgtcggcggt ggtgttcgcg
      781 ccgccgccgt gccaaagcga cccgtggaag atcatgaccg agccggcggg catctccagc
      841 ttctcgatcg tgacgtcgtc gagcgcgccg ggtgcgtcgg gcgttcctgc gaactggtgc
      901 gaacccggaa cgaaccaggt gccgccgttc gccgccgtga agtcggtgag cgcccacatg
      961 ctcgtgacga acatcggcgc gtgcggtcgc tgcaagtggc gcgacatcac gatgtcgtcg
     1021 ccgtgcatgg gctgcgggtg ttctccaggt ccgatgtcga tcgcagtcat gcccgagagc
     1081 aggcagccac catcgagcag ccgctcgacg atcgggagca cacttccgtg caccggcatc
     1141 gcttggaaca ccggatcctt cgccagcaag ttgtacatcc gcagcgtcgc gtgcccttcg
     1201 gccgaggtgt cgcgcgggcg caggtcgagc tcgcgttcca accgccggat cgtgtcgcgc
     1261 agattggtca cgaggtccgg ttcgatcgcg tcattgacga tcgtgtagcc gtcggcctcg
     1321 atgcgggcaa cgtgttcgga aattcccggc tcggtcagcg tcacacctga caccatggcg
     1381 gacgtggagc cgctcgacaa ccccatatgg cacgcgctga cgggcccgca ggcgatgttt
     1441 tcggaaggaa ccggcctcgc gctgcgcttc gaacccgagg tggcggtctt cgccgccttg
     1501 cccgaccagg tcgccgccga cgcatgggac gcgctacgcg cgctggtcgg gaacggcggc
     1561 gcaattgttg ctcgtccgca actggagccg ccgccgggtt ggcgcagccc cttccggctc
     1621 gacggattcc agatgatcgc ccctgacagt ttcggccgcg gcgcgcccga cgatcggttc
     1681 gtgcggctca cgaacgacga cgttcccgaa atgctcgcgc tggtcgaacg aacccgaccc
     1741 ggaccattcg cgaaacggac gatcgagctg ggcccgtact acgggttgcg cgacgacgcg
     1801 ggcgaactcg tcgcgatgtc gggcacgcgc atgcgcctcc ccgggtacgc cgagctcagc
     1861 gcggtgtgca ccgaccccgc gctgcgaggg aaaggcatcg cgaccgccct gatggaaacg
     1921 gtcgccgccg gcatgcgcag caacggcgag caaccgatgc tgcacgttgc cgagaacaac
     1981 tcgaccgcga tcgcgctcta cgagcatctc ggattcgtgc gccggtctcg cttcacgttc
     2041 gcgttggtgc aaccgcctga atgatggcct caacgatcgc gtcgggcggg cggccggcgt
     2101 cgacgatcgt tgtcgcgagc atgcgataga tcggatcgcg tgcggcgtac tgcgcccgca
     2161 gcagttcgag cgtgtcaccc gcgtcgacga agggacgatg gccgccgccg cctaccaggc
     2221 gcggcgcaag cgtcgacggc tctccgtgca ggtaaacgac atggtgcccc gcgaactgct
     2281 cggccccgtc gacgagcggc gcggccgccg cagccgtgat gacggccggg accggatcgg
     2341 acagggcgtc ggcgaggacg gctcgttcca gctggtgcag cgcatcgacg ccgtcggcag
     2401 cggcgatgtc acgcgcccgg cgaccggttc gtcgttccag catgacgtcg ttgtcgacaa
     2461 aggcgaagcc gaggcacgcc gcgagcggac gaccgacggt cgacttccct gatcccatca
     2521 acccgatgag cacgacgtgc acgggcgtcc tccagtcggc ctcggaaggg cgttccccgc
     2581 tagattcttc cctcatgcgc gtgccgccgg ctcgggtgtt gttcccggaa gaagaccgcg
     2641 ccgaaatcct ggcccgaatc gacgaagccc tggccaccgg ccagttgacc ctcggaccgc
     2701 tcggccgcga cctcgaagcc gcgttcgcgc agcgccatca gcgcgagcac gcaatcgcgg
     2761 tcagttcggg cacgagcgcg ctggagatca tcctccgagc catcggcgtc gagggccgcg
     2821 aggtcctggt cccggccaac acgttcttcg cgactgccgc ggccgccgcc cacgcgggcg
     2881 cgcgcgtcaa gtttgtcgac tgcaacaccg acacgatggc gtttgacctc gacgacgtcg
     2941 cggcccagat cggtccgcag accgcggccg tcattgcggt gcacatcggc gggctcatca
     3001 gccccgacgt tccggcgctg gccgaactgt gcgcgcagca cggcgtcgag ctggtcgaag
     3061 acgccgccca cgcgcacggc agcgcgctcg atgggcacgc ggcgggcacg ttcggaatcg
     3121 cgggcgcgtt ctcgttctat ccaacgaagg tgatggccgg cggcgaaggc ggcatgattg
     3181 tcacgaacaa cgcgacgatc gacgaagaag cgcgtaccta ccgcgatcaa ggtaagggat
     3241 cgttcctctc gaacttccac actcgcctcg gtgcgaactg gcgcatgagc gaaccgcacg
     3301 ccgcgatcgt gcatgcacag ctacggcgcc tcgacgagtt catcgccgct cgtcagaacc
     3361 tggcgaagcg ctacgacgcc gtcgtggagt cgatcggctt gcagccgttc attagtccgc
     3421 ccaacgcgca ctgcaactac tacaagtaca tcgcgttcct gcccgagggc gtggaccgca
     3481 ccgatgtgaa gcaggccatg cgagagcgct tcgacgtcgg tctctcgggc gaggtctacg
     3541 acacgccgtt gcatcgtcag ccggtcttcg agacgttcgc cgatcgcgag ctgccgaatg
     3601 ccgagtggct gtgtgcgcgc cacatctgct tgccgctgta tccgtcgctg tccgacaccg
     3661 acgccgacta cgtcatcgaa tcgctggcca ccgtgctcgc cgagcgcggc gtgcccgcgt
     3721 aagaaccgga gcttttgtgc aggacaactt gattgcggtg accggcggtt ccggttttgt
     3781 ggggtcgcat gtcgtcgacg cacttctggg cgcgggctac gaagtgcggg tcatcgatcc
     3841 caagaagccg taccagcccg aagcagattg ggccgacgtc gacatgctcg accaggacgc
     3901 ggtcaccgac gcggtgaagg gcgcgagcgc ggtgttccac ctcgccgcca tggccgacgt
     3961 caacgacatc gtcgccgatc cggccggcag cgtcgcgctc aacaccctgg gcgtcgcacg
     4021 ggtgctggaa gccgggcggc gcgccgacac cggccgcgtc atcgtgtcga gcacggtgtg
     4081 ggtgtacgcc gcaacgcacg gcgacgtcgt cgacgagacg acgctgttcg acctcgagac
     4141 cgaccggcat ttgtatgtgt cggagaagat tgcagccgag atgttctgcc gcgactacgc
     4201 gacgctgttc ggacggccgt acacctgcct gcgctacggc atcccgttcg gcccgcgcat
     4261 gcgcagcgac ctc
//
LOCUS       DASUGA010000330         2863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1273466, whole genome shotgun sequence.
ACCESSION   DASUGA010000330 DASUGA010000000
VERSION     DASUGA010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273466"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..658
                     /locus_tag="VFX21_06550"
     CDS             2..658
                     /locus_tag="VFX21_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095649.1"
                     /translation="MPLDTPHPRLDVLAERGFVVLRDADFEIPDDEFRSLEYMDWKSG
                     GDTNFAPIATADGQLDCRGFWNKGDERPDKGARFTSNAEKCPTIKRYVESVGADFGRV
                     RTIKLEPQDHDTAFRSFHRDDNNRFNPEADGWVVRAWFELTDFPESYMLLMDTGPDGL
                     PDSATEVRLPLYKGARFVVDTQRLWHVVVHNGNAPRYALITSFESGPALDGWITSQLP
                     "
     gene            670..1818
                     /locus_tag="VFX21_06555"
     CDS             670..1818
                     /locus_tag="VFX21_06555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5095650.1"
                     /translation="MRFGLFMAPFHPTGQNPTLALERDLQLLEHLDALGFHEAWIGEH
                     HSGGYEIIASPEVFIAAAAARTKHIRLGSGVNSLPYHNPLMLADRFVLLDHLTRGRAM
                     LGCGPGQLTSDAHMLGIPADEQRPRMNESLEAIMALLNGETVTMHTDGFTLQDARLQL
                     RPYSEPNLEVAVAASISPTGAVAAGKHGIGLLSIAATTSQGFDAIGTHWNTWKETAEQ
                     HGHVADRSKWRLVGPMHIAETKEQARKDVEYGIVPFSKYFTHVLPAGPTRGDTAEEII
                     ANTAEDGFAIIGTPDDAIAKIQSLVDQSGGFGTFLLFGHDWASPAATKRSFELFAQYV
                     MPHFTDQLGAPQASCDWVTGSGGEFVNRAIGAITKAIVDHAESQNPVS"
     gene            complement(1811..2413)
                     /locus_tag="VFX21_06560"
     CDS             complement(1811..2413)
                     /locus_tag="VFX21_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095651.1"
                     /translation="MDLVVDDRGVHATPKTVPAGHVEVTMADQRSDKTHPVSVRSVPP
                     PLGMTVGTKLIALRIAGRGYALTADVNGEPLPEGDSVSVVVPELAAPQEPAHQVTVEV
                     AEDGITTPARETRRGQPLQLTTENEPPMAQPWTTVVPGKAEIVVRNRTGAEQQCSVGD
                     ESVSVDETATLITQLGGDDGFATLTCAGGGRSFSFGLWFS"
BASE COUNT          494 a          998 c          905 g          466 t
ORIGIN      
        1 gattccgctc gacacaccgc acccgcgact cgacgtgctc gccgagcgcg ggttcgtcgt
       61 tctgcgcgac gccgacttcg aaattcctga cgacgagttc cggtcgctgg aatacatgga
      121 ctggaagagc ggcggcgaca cgaactttgc gccgatcgcc actgccgacg gccagctcga
      181 ctgccgcggg ttctggaaca agggcgacga gcgacccgac aagggcgcac gcttcacgag
      241 caacgcggag aaatgcccga cgatcaagcg ctacgtggaa agcgttggcg ccgacttcgg
      301 acgcgtgcgg accatcaagc tcgaacccca ggaccacgac acggcgttcc gcagcttcca
      361 ccgcgacgac aacaaccggt tcaatccgga ggccgacggc tgggtcgtgc gcgcgtggtt
      421 cgagctcacc gacttccccg agagctacat gctgttgatg gacaccggcc ccgacgggct
      481 ccccgactcc gcgaccgagg tgcggctgcc tctgtacaaa ggcgcgcggt tcgtcgtcga
      541 cacgcagcgc ctgtggcacg tcgtcgtgca caacggcaat gcgccgcgct atgcgctgat
      601 caccagtttc gagagcggtc ccgcactcga cgggtggatc acctcgcaac tcccctagtc
      661 tccgcagcca tgcgcttcgg cttgttcatg gctcccttcc acccgaccgg gcagaacccc
      721 acgctcgcgc tcgaacgcga cctccagctg ctggagcacc tcgacgcact cgggttccac
      781 gaggcctgga tcggcgagca ccactccggc ggctacgaga tcatcgcctc gcccgaagtg
      841 ttcattgcgg ctgcggcggc acgcacgaag cacatccgcc tcggcagcgg cgtgaactcg
      901 ctgccgtatc acaacccgtt gatgctggcc gatcgtttcg tgctgctcga ccacctcacg
      961 cgtgggcgcg cgatgctcgg ctgcggtccc ggccagctca cgtccgacgc gcacatgctc
     1021 ggcatccccg ccgacgagca gcgcccgcgc atgaacgagt cccttgaagc gatcatggcg
     1081 ttgctcaacg gcgagaccgt gacgatgcac accgacggct tcacattgca ggacgcgcgg
     1141 ctgcagttgc gtccatacag cgaaccgaac ctcgaggttg ccgtcgcggc gtcgatctcg
     1201 ccgaccggcg ccgtcgcggc cggcaagcac ggcatcggcc tcctttccat cgcggccact
     1261 acgagccagg gcttcgacgc gatcggtacg cactggaaca cgtggaaaga aactgcggaa
     1321 cagcacggcc acgttgccga ccggtcgaag tggcgcctcg tcggcccgat gcacatcgcc
     1381 gaaacgaagg aacaggcacg caaggacgtg gagtacggca tcgtgccgtt ctcgaagtac
     1441 ttcacacacg tgctgccggc cggcccgacg cgcggtgaca ccgcggaaga gatcattgcg
     1501 aacaccgcag aggacggctt cgcgatcatc ggcacacccg acgacgcaat cgcgaagatc
     1561 cagtcgctcg tcgatcagtc cggcgggttc ggcacgttct tgttgttcgg ccacgactgg
     1621 gcgtcgcccg ccgccaccaa gcgcagcttc gaattgttcg cgcagtacgt catgccgcac
     1681 ttcaccgatc agctcggtgc gccgcaggcg tcatgcgact gggtcaccgg cagcggcggc
     1741 gaattcgtca accgggccat cggcgcgatc acgaaggcaa tcgtcgatca cgccgagtcg
     1801 cagaacccgg ttagctgaac cagaggccga acgagaacga gcggccgccg ccggcgcacg
     1861 tcagcgtcgc gaacccgtcg tcgccgccga gctgcgtgat cagcgttgcg gtctcgtcga
     1921 cactgaccga ctcgtcaccg accgaacact gctgttcggc accggtgcgg ttgcgtacga
     1981 cgatctctgc cttgccggga acgacggtgg tccacggctg cgccatcggc ggttcgttct
     2041 cggtcgtcag ctgcaacggt tgaccccggc gcgtttcccg cgccggcgtg gtgatgccgt
     2101 cttcggcgac ttcgaccgtc acctgatgcg cgggctcttg cggcgcggcg agctccggca
     2161 cgacgactga caccgagtcg ccttccggca gcggctcgcc gttgacgtcg gccgtgagtg
     2221 cgtagccgcg gccggcgatt cggagcgcga tgagcttcgt cccgacggtc atgccgagcg
     2281 gcggtggcac cgaccgaacc gagacgggat gtgtcttgtc ggagcgctgg tcggccatcg
     2341 tgacttcgac gtgcccggcg ggcaccgtct tcggcgttgc gtgcacaccg cgatcgtcaa
     2401 cgaccagatc cacgtgactg aacgtctcgg caggacgcgc cttcggttcg gtcggcgcct
     2461 cgttgcgcgc gatcagaaga accagtcccg ccaagaacaa cacaatgacc gcgtcaaccg
     2521 cccaacgcgc agctctactc actgcaactc cctcgatgcc ctgcttcgca tggtgcagca
     2581 ctcgcccgac cggtgacatg ggtcgccggg cccattcgta ggatgcccgg cgtgtctcct
     2641 gtcacgactc ggctcgccac gaaggaattg tcgctcgcaa tccactcgtg gggaggtgat
     2701 ggtgcgcccg tgctgctttc tcatgccacc ggctttcacg gccaggcctg ggcagaggtt
     2761 gcgcagcaac tggtcgaagc gggccgccaa gtgtggtcat tcgacttccg cgggcacggc
     2821 gacagtgacg cgccgtcgct cgccgtcgac gactacgcgt ggg
//
LOCUS       DASUGA010000331         2466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1868009, whole genome shotgun sequence.
ACCESSION   DASUGA010000331 DASUGA010000000
VERSION     DASUGA010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1868009"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..983)
                     /locus_tag="VFX21_06565"
     CDS             complement(147..983)
                     /locus_tag="VFX21_06565"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX5095652.1"
                     /translation="MERAPHPWNRAFTWADHVDPPATITRAQMASYDRDGFFVFEDAF
                     DAATLTELDAELAPGDAGVKEFLAKMPDGRYSVAGLDTQTVAPHAVMRSEYAREFVAH
                     PVLAGIARDLVGPDVRLYWEQSVYKQPQSAEPVLWHQDNGYTYTEPQAYLTCWIAITD
                     ATLENGCVSVMPGVHREGTLAHRNTPVGEECWGDWDAAVDVPVRAGSIGVFTSLTPHA
                     TRRNRTDKVRKAYIVQYAPDGAVAHFGDANAGPPVKTEVLADDRRFWIVRDGERCATA
                     SA"
     gene            complement(1008..1304)
                     /locus_tag="VFX21_06570"
     CDS             complement(1008..1304)
                     /locus_tag="VFX21_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5095653.1"
                     /translation="MRESGRTVKLEIHGEYDLACGDAAKTSSEHVAERLSREMDTLVV
                     DLSAMTFFDAAGIAYLLRLEHIARIVSADYRVEGASSTVRRLLEIVELDHVLAE"
     gene            complement(1355..1759)
                     /locus_tag="VFX21_06575"
     CDS             complement(1355..1759)
                     /locus_tag="VFX21_06575"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5095654.1"
                     /translation="MSSAGADNETAVFPPTDDAPYESRSLVRNFLTDHDADDLVDIGL
                     VLTSELVTNVIRHAHSPVQLDLEWQAETLRVEVRDGSSIIPAVAELANADGGFGLRLV
                     DRLAADWGIRQLDDGKAVWFTVRREHPPGDSA"
     gene            1851..>2466
                     /locus_tag="VFX21_06580"
     CDS             1851..>2466
                     /locus_tag="VFX21_06580"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEX5095655.1"
                     /translation="MSRGKSSAVLLVLALVAGAACSSSSKSSDDKPAASTTTTAAPRP
                     SSGPAATLTAPENAGKGVYLAAGQAVDMAGDGYTESEFFASGEAHSYSLSTEQTSDGK
                     WGAVESGAAAPYETRVVLRRPKDPAKFNGTILYEWLNVSGGVDASPDFTYLRDEIVRE
                     GYAWAGVSAQHIGVEGGEAVVAVSAAPKGGLKGIDPARYGELHHP"
BASE COUNT          387 a          843 c          831 g          405 t
ORIGIN      
        1 cgcctcggcg catggacgaa gctggccgtc gaaccgcaac gcgatgtgca cgtcggccgg
       61 cgctacttcc gcagcggcca ccgcgtcctc gacgcgctca ccgagcgcct caagcgcgaa
      121 ctcccaattc ggtaggtgcg acgcacctac gcgctggcgg tcgcgcagcg ttcgccgtcg
      181 cgcacgatcc agaagcgacg gtcgtcggcg agcacttccg tcttcaccgg cgggcccgcg
      241 ttcgcgtcac cgaaatgggc gactgcaccg tcgggcgcgt actgcacgat gtacgccttg
      301 cggactttgt cggtgcggtt acgccgcgtc gcgtgcggcg tgagcgacgt gaacacgccg
      361 atgctgccgg cacgcaccgg cacatcgacc gctgcatccc agtcgcccca gcactcttcg
      421 ccgaccggcg tgttgcgatg cgcgagcgtg ccttcccggt gcacgcccgg catgaccgac
      481 acgcaaccgt tctcgagcgt cgcgtcggtg atcgcgatcc agcacgtgag gtacgcctgc
      541 ggttcggtgt acgtgtagcc attgtcctgg tgccacaaca ccggctccgc gctctgcggc
      601 tgcttgtaca ccgactgttc ccaatagagg cgaacgtcgg gaccgacgag atcgcgcgcg
      661 atgccggcca gcaccggatg cgccacgaac tcccgggcgt actcactgcg catcaccgcg
      721 tgcggggcga ccgtttgggt gtcgagtccc gccacgctgt agcggccgtc gggcatcttg
      781 gcgaggaact ccttcacgcc cgcgtcgccg ggcgcaagct cggcatccag ctccgtgagc
      841 gtggcggcgt cgaacgcgtc ttcgaacacg aagaatccgt cgcggtcgta gctcgccatt
      901 tgggcacgcg taatcgtggc cggcgggtcg acgtggtctg cccacgtgaa cgctcgattc
      961 cacggatgcg gtgcccgctc cacggggcag cacggtagcc gttgcgtcta ctcggccaac
     1021 acgtggtcca actcgacgat ttccagcagc ctgcgcactg tggagctcgc accctcgact
     1081 cgatagtcgg cggacacgat tcgcgcgatg tgctcgaggc gcaggaggta cgcgatcccg
     1141 gccgcgtcaa agaacgtcat cgccgagagg tccacgacca gcgtgtccat ctcgcgcgac
     1201 aggcgttctg ccacgtgctc gctggaggtc ttcgcggcgt cgccgcaggc gaggtcgtat
     1261 tcgccgtgga tttcgagctt caccgtccgg ccgctctcgc gcacgacgaa cttggcgtga
     1321 cgcgctttgc agacgaacgc gccgccctcc ggaatcatgc agagtccccg ggcgggtgtt
     1381 cacgccggac ggtgaaccac acggccttgc cgtcatcgag ttggcgaata ccccagtcgg
     1441 cggcgagccg gtcgacgagc cgcaaaccga aaccgccgtc cgcattcgcc agctcggcaa
     1501 ccgcgggaat gatcgacgag ccgtcgcgca cctcgacacg cagggtctcg gcttgccact
     1561 cgagatcgag ctgcacgggg gagtgcgcgt ggcggatcac gttcgtgacc agctcgctgg
     1621 tgagcaccag cccaatgtca accaggtcat cggcgtcgtg gtcggtgaga aagttgcgaa
     1681 cgaggctccg gctctcgtag ggcgcatcgt cggttggcgg gaagacggcc gtctcgttgt
     1741 cggcgccggc actgctcata tctccgccct accgcgacag ccgacggtcg taacgctcga
     1801 cccttgttgg gcgcgcatgc ggtttgaccg cattgtgcga gactcctccg atgtctcggg
     1861 ggaaatcgtc tgctgttctg ctcgtgctcg cgctcgttgc gggagcggcc tgctcgtcgt
     1921 cgtcgaagtc gagtgacgac aaacccgcgg cgtcgaccac gacgaccgcg gcgccgcggc
     1981 cgtcgagtgg accggccgcg accctgactg cgccggagaa cgccggcaag ggcgtgtatc
     2041 tggccgccgg ccaggcggtc gacatggccg gcgacggcta caccgagagc gagttcttcg
     2101 cgagcggtga agcgcactcg tactcgttgt cgaccgagca aacgtcggac ggcaagtggg
     2161 gcgccgtcga gtccggcgcc gcggcgccct acgagacgcg agtcgtgctc cgccggccga
     2221 aggatcccgc caagttcaac ggcacgatct tgtacgagtg gctgaacgtg agcggcggcg
     2281 tcgacgcgtc acccgacttc acctacctgc gcgacgagat cgtgcgcgag ggctacgcgt
     2341 gggccggtgt ttcggcccag cacataggcg tcgaaggggg cgaggcggtc gtcgcggtgt
     2401 cggccgcgcc gaagggtggc ctcaaaggca tcgatcccgc tcgctacggg gagctgcacc
     2461 accccg
//
LOCUS       DASUGA010000332         8410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_698819, whole genome shotgun sequence.
ACCESSION   DASUGA010000332 DASUGA010000000
VERSION     DASUGA010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8410
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698819"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VFX21_06585"
     CDS             <1..582
                     /locus_tag="VFX21_06585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5095656.1"
                     /translation="GQIEVLRRPKKIGLGDAYRAGFTVGLERGYDVLIQIDADLSHDP
                     AALPGMLQQLEHGADVVVGSRYVPGGSIPHWPWFRRALSKWGNRYATLVLGLKVNDAT
                     SGYRVYRAPILSKVDYATTRSKGYGFQIELAYRVMREGGDIEQAPITFTDRVRGHSKM
                     TWKIAVEELTLVTWWGFRDRVLHRGKKAEQHAA"
     gene            complement(724..2181)
                     /locus_tag="VFX21_06590"
     CDS             complement(724..2181)
                     /locus_tag="VFX21_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013873361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5095657.1"
                     /translation="MRWLSSNHEPTDVVVIGAGPAGLTAAYELAKRDVAATVLEADGI
                     VGGISRTEERDGWRFDIGGHRFFTKVRAVEDLWFEILGPDDFLRRPRLSRIYYRGKFY
                     DYPIVPLKALRSLGFVEAMRCGLSYVWVRIKPPKDKTTLEGFVASRFGWRLYRHFFKT
                     QSEKVWGVPCTEIQADWGAQRIKNLSLFRALWEAIKPKRVRANASKSNQVTSLIEEFN
                     YPKYGPGMMWERCAELVTAMGTKITMDSAVTRIERRDGRAVAVWAVTDGVETRYDCTH
                     VISSMPIGALLRAMDPPVPDDVRAAADALRYRAHITVGLVVPEADSFPDNWVYVNDPH
                     VKVGRIQNFGRWSPYLVKDGRTCLGLELFVDEGDEWWTKSDDELIATGSRELGEIGLI
                     DPDDVEAGYVIRMPKAYPFYDAEYKAHVETLRAWLTEHTPNVYPVGRNGMHRYNNQDH
                     SMLTAMLSVENIFGASHDIWSVNVEAEYHEEKTGA"
     gene            2227..4269
                     /locus_tag="VFX21_06595"
     CDS             2227..4269
                     /locus_tag="VFX21_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095658.1"
                     /translation="MSSALKRPAIFGALASGVVFVAVLADRHWSVLRYDTVGGFYDLQ
                     AHRFLQGHLDVPADPLGYEGIFIGRRVYMYYGPWPAILRLPVAVFTHRFDGRLTQLSM
                     LLAFAVALFFTIRLFQRVRTLVAGETAVTRGERWATGVFVFVVGAGSVLTFLASVGWV
                     YHEAEIWGVALALAGFDFVVAYLVSRRMRDLVWASFFATLAFWARASVGAGPAAALAL
                     LLIAQISEPTRRLVAMPATGSVVRAARNLAVATVVPFGAYAAVNYAKFHSLFSVPLDR
                     QIRYVAVNESRRAALADNGGSLFGLKFVPTTLGQYLRPDGIRVNSVFPWVSFPPRASI
                     IGNATFDTIDLSSSVTACMPALAVLALVGLVAVVRARGRDAPNVAVLRAPIAGAALAT
                     GATVTIGFIANRYLSDFVPLLVLGAIAGFHVTLVWLRGPGTRGQRAMKRTVGVALVVL
                     ASFSLWANVGLAVLYERTLAPTEIDEWSLAGFIGFQDRVHDIVPGGALPNVRHGASLP
                     VDVAALGTVFVVGDCDGLYWSNGDRWQAVERTHTTGLWRVRATFEPAGNEWQPLLTNG
                     VPGADAYLGVRVRDDGRVVFGVAAPVSDGEWSEGEPQDIPLGTPMTVDLIYDPSFGRA
                     VVRVEGRIVLGYVTPLPPRQDVSIGRNDITDETVPTFAGALSDLPVTPTLCHRLTR"
     gene            complement(4235..6046)
                     /locus_tag="VFX21_06600"
     CDS             complement(4235..6046)
                     /locus_tag="VFX21_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095659.1"
                     /translation="MVRDAAPARAGNRGHAVVLETALYTIVVVCTTWPALTRSRDEML
                     GGSDDARYYAWLGWRVSRLIADGHVLPAHIPDVIAPFGLDARLLDGYLPTYVCALWNL
                     VTGPVLAYNLTLITGVALNLLAARALARRLTGNRAVVMIGAVAFATAPALALNAQVGV
                     PPLLWAWTLPLFVGEALDVVTRRSPVRPVRLALLFVAAYLCSIYFLVFGAVVYAAIVF
                     VGAVRDHEWRPIAMTALSAAAAFVLLTPFVVPRLTFDREDTRHDDDALLAESNLYSAD
                     ALSLVAQPTRSTVLLPRPTVVDDSIGRLPDPTHALESTLFPGFLVLGGFVTFMFVRDR
                     RRLPLAVAAAAVFVLALGPSLKVGGRFLWRDDDQPVSWLPFRLLSQIPGLGALRAPAR
                     ASYALVAVGVAATVIVLERLLARAPQRAPFVVAASVLLLGTNLLIPLPSVDMQTTPTS
                     DDALRQIARIADPGDTVLRVPADCDPDFVAYQLLHHTASVGCAGSSAANPWHTKMTAY
                     AASDAFTKLRCDRRTYGRLETTPIATAPFGPDDVDELRAQFGVRFLIVDRARLGADRC
                     PAVHDLDDVLSRYEILGADDRFAIIDLSGSGGDTTSG"
     gene            complement(6054..7211)
                     /locus_tag="VFX21_06605"
     CDS             complement(6054..7211)
                     /locus_tag="VFX21_06605"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX5095660.1"
                     /translation="MSGIHQGHQRFPCFEGIRAIAALSIIVYHSMFFTSLFFDIWGGS
                     VWANLNAGVWVFFVTSGFLLYLPFAAHHLGKAPAVDTGRYAARRAARIYPAYWVVVAF
                     FTLVIAKAQIFGFDGFLRHFSLTQTYTAEKNPFLVGLPPAWSLVVEVTFYVLLPLYAL
                     GVAAGARRSRPIVAEFVGAGLFAGVGVLAIVAVAAGFDPVWMTVFPQHALPFGLGILL
                     AVCVTHDWPAAVAERFAGAGERSWIWWCGALAALLAIPFLAGIDPGERLSSAQIVELN
                     LLQTAIGFCIVVPAALGRQRTRGIRRALQTPVLVYLGVVSYGLYLWHWWVLGIVQHDW
                     LGWDVNSGNWLALLALGLPVVVGAATLSWFVVERPIIGLARRARRRPVPAA"
     gene            complement(7208..>8410)
                     /locus_tag="VFX21_06610"
     CDS             complement(7208..>8410)
                     /locus_tag="VFX21_06610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX5095661.1"
                     /translation="ANVIGIVALAGVVVTMRIAAENTLFLYRSGFFWFSVASAVVIAV
                     IVRFPSTPLSRFLGLGPLVAIGLRSYSIYLWHWPVRVFVTGEKVHLHGTALFLVRLTI
                     LVVLSEASYRLVERPFRTGRIARRTGSRGAIAAFVGLIALTFVLVQTAARPRPLPPTS
                     LAAVSVTAPTTDGTESAPQRKFDIYGDSTGLVFGLSAAGHLGELQGISGVGGQAMLGC
                     GLVETDQYAAGRIIERPQVCDGIDDRWRITIAAEPDAVRVIHTGAWEVLDHRVDGKTV
                     RYGTAEWTELVRSSYERAFDLLTNDGRTVFVFQTPCYGDGDPAYPLPERAQPQRVDAI
                     NVIIDELAAANSRVVVVPWRDVVCPGGTRAEALDGADLWEADGVHLTDDGAVAVWKWF
                     LGWLDARE"
BASE COUNT         1410 a         2856 c         2841 g         1303 t
ORIGIN      
        1 gggcagatcg aagtgctgcg ccgcccgaag aagatcgggc tcggtgacgc gtaccgcgcc
       61 gggttcaccg tcggcctcga gcgcggctac gacgtgctca tccagatcga cgccgacctc
      121 tcgcacgatc ccgccgcgct ccccggcatg ctgcagcagt tggagcacgg cgccgacgtc
      181 gtcgtcggct cgcgctacgt gcccggcggc tcgatcccgc actggccgtg gttccgccgg
      241 gcgctgtcga agtggggcaa ccgctacgcc acactcgtgc tcggattgaa ggtcaacgac
      301 gcgacgtccg ggtaccgcgt gtatcgcgca cccattctca gcaaggtcga ctacgcgacg
      361 actcgctcga agggctatgg cttccagatc gagctcgcgt accgcgtgat gcgcgaaggc
      421 ggcgacatcg agcaggcgcc gatcaccttc accgaccggg tgcgcgggca ctccaagatg
      481 acgtggaaga tcgcggtcga agagctgacg ctcgtcacct ggtggggctt tcgagatcgc
      541 gtcctgcacc gcggcaagaa ggcggagcag cacgccgctt gagccccggc gcgtgcggat
      601 tcgccccctt cggcgcagcc gcctccagcc cattcgcacg agctccgacc gccggccgag
      661 cgagcgaggc ttcgtcggtc ggaacagaga ggcgtcggcg cagccgtcca cgcccttccg
      721 gcgctacgcg cctgtcttct cttcgtggta ctcggcctcg acgttcaccg accagatgtc
      781 gtgagacgcg ccgaagatgt tctcgaccga gagcatcgcg gtgagcatcg agtggtcctg
      841 gttgttgtag cggtgcatgc cgttgcgccc gaccggatag acgttcggcg tgtgctcagt
      901 gagccacgcg cgcagcgtct ccacgtgcgc cttgtactcg gcgtcgtaga acggatacgc
      961 cttcggcatc cggatcacgt agcccgcttc gacgtcgtcg ggatcgatga gcccgatctc
     1021 ccccagctcg cggctgccgg tggcgatgag ctcgtcgtcg ctcttggtcc accactcgtc
     1081 gccttcgtcg acgaacagct cgagcccgag gcaggtgcgg ccgtccttga cgaggtacgg
     1141 cgaccagcga ccgaagttct gtatccggcc taccttgacg tgcgggtcgt tcacgtagac
     1201 ccagttgtcg ggaaagctgt cggcttcggg gacgacgagg ccgacggtga tgtgcgcgcg
     1261 gtagcgcagg gcgtcggcag cggcgcgcac gtcgtcgggc accggcgggt ccatcgcacg
     1321 caagagagca ccgatcggca tcgacgagat cacatgcgtg cagtcgtagc gcgtctcgac
     1381 accgtcggtg accgcccaca ccgcgaccgc ccttccatcg cgccgttcga tgcgggtcac
     1441 tgcggaatcc atcgtgatct tcgtgcccat cgcggtcacc agctcggcac agcgttccca
     1501 catcatcccg ggtccgtact tcgggtagtt gaactcctcg atgaggctcg tgacctggtt
     1561 cgacttcgag gcgttggcgc gcacccgctt cggcttgatc gcctcccaca gtgcgcggaa
     1621 cagcgacagg ttcttgatgc gctgggcacc ccagtcggcc tggatctcgg tgcaaggcac
     1681 gccccacacc ttctcgctct gcgtcttgaa gaagtgtcgg tagagccgcc agccgaagcg
     1741 cgacgcgacg aaaccctcga gcgtggtctt gtccttgggt ggcttgatgc gcacccagac
     1801 gtacgagagg ccgcagcgca tcgcttcgac gaagccgaga cttcgcaacg ccttcagcgg
     1861 gacgatcggg tagtcgtaga acttgccgcg gtaatagatg cggctcagtc gtgggcgccg
     1921 gaggaagtcg tcgggaccga ggatctcgaa ccaaaggtcc tcgacggctc gcaccttggt
     1981 aaagaaccgg tgaccgccga tgtcgaagcg ccagccgtcg cgctcttcgg tacggctgat
     2041 gccgccgacg ataccgtcgg cttcgagcac cgtcgcggcg acgtcgcgct tggcaagttc
     2101 gtacgccgcg gtcaggccgg cgggacccgc accgatgacg acaacgtccg tgggctcatg
     2161 gttcgaggac aaccagcgca gtctatgaaa cagtgacgcg ggtcctcggt tccgtatagt
     2221 ccgcccgtgt cgtccgcgct gaaacgacct gcgatcttcg gcgccctcgc gtccggagtg
     2281 gtcttcgtcg cagtgctggc cgaccgtcat tggtcggtct tgcggtatga caccgtcggg
     2341 gggttctacg acctccaggc gcaccgattc ttacagggcc atctcgacgt ccccgccgac
     2401 cccctcggct acgaaggcat cttcatcggc aggcgcgtct acatgtacta cgggccgtgg
     2461 ccggcgatct tgcgcctgcc cgtcgccgtg ttcacgcatc gtttcgacgg ccggctgacc
     2521 caactctcga tgctcctcgc gttcgccgtg gccctgttct tcacgattcg cctcttccag
     2581 cgcgtccgca cgctcgtcgc cggcgagacc gcggtgacgc ggggtgaacg gtgggcgacc
     2641 ggcgtgttcg tgttcgtcgt cggcgcggga tcggtgctca cgttcctcgc gagcgtcggt
     2701 tgggtgtacc acgaagccga gatctggggt gtggcgctcg cgctggccgg gttcgacttc
     2761 gtcgttgcgt acctcgtttc gcgccggatg cgcgacctgg tctgggcatc gttcttcgcg
     2821 acgctggcgt tctgggcgcg cgcctcggtc ggtgcgggtc ccgcggcggc gctcgcgctg
     2881 ctgctgatcg cccagatctc ggaaccgacg cgacggttgg tcgcgatgcc cgccaccggc
     2941 tcggtggtcc gcgccgcgcg caacctcgcc gtcgcgacgg tggtgccgtt cggtgcgtac
     3001 gcggcggtca actacgcgaa gttccacagc ctgttctcgg tccctctcga tcggcagatt
     3061 cgctatgtcg ccgtcaacga gagccgccgc gccgcactcg ccgacaacgg aggcagcctg
     3121 ttcggcttga agttcgtacc gacgaccctc gggcaatacc tgcggcccga cgggatccgt
     3181 gtgaacagcg tgttcccttg ggtgtccttc ccgccgcgcg caagcatcat cggcaacgcc
     3241 acgttcgaca cgatcgacct gagctcgagc gtgacggcgt gcatgccggc ccttgcggtt
     3301 cttgcgctcg tcggcctcgt tgcggtcgtt cgtgcgcgcg gtcgcgacgc gcccaatgtc
     3361 gcggtgctcc gggcgccgat tgcgggcgcc gcgctggcaa ccggtgcgac ggtgacgatc
     3421 gggttcatcg ccaaccgata cctgtccgac ttcgtaccgc tgctcgtcct cggagcgatc
     3481 gccggcttcc acgtcacgct tgtgtggctg cgcggaccgg ggacgcgcgg gcaacgcgcc
     3541 atgaaacgca cggtcggtgt cgcgctcgtc gtgttggcat cgttctccct gtgggccaac
     3601 gtcggtctcg cggtgctcta cgagcgcacg ctggcgccca cagagatcga cgagtggtcc
     3661 ctcgccggct tcatagggtt ccaggatcgt gtgcacgaca tcgtcccggg cggcgcgctg
     3721 ccgaacgtgc gacacggcgc atcgctcccg gtcgacgtcg cggcgctcgg cacggtgttc
     3781 gtcgtcggtg actgcgacgg gttgtactgg tcaaacggcg atcggtggca ggcggtcgag
     3841 cgtacgcaca cgaccgggct atggcgtgtg cgggcgacgt tcgagccggc aggcaacgag
     3901 tggcagccct tgctcacgaa cggcgtgccg ggcgccgatg cgtacctcgg cgtgcgtgtg
     3961 cgcgacgacg gacgggtcgt cttcggcgtc gcggcgccgg tcagcgacgg cgagtggagc
     4021 gaaggcgagc cccaggacat cccgctcggg acaccgatga ccgtcgatct gatctacgac
     4081 ccatcgttcg gccgcgcggt ggtgcgcgtc gagggacgga tcgtgctcgg gtacgtcacc
     4141 ccccttccgc cgcgacagga tgtctcgatc gggcgcaacg acatcaccga cgagaccgtc
     4201 cccacgttcg ccggtgcgct gagcgacctg ccggtcaccc cgacgttgtg tcaccgcctg
     4261 acccgctgag gtcgatgatc gcgaatcggt cgtcggcacc gagtatctca tagcgcgaga
     4321 gcacatcgtc gagatcgtga acagccgggc atcggtcggc acccaaacgc gcgcggtcaa
     4381 cgatcaggaa ccgcaccccg aactgtgcac ggagctcgtc gacatcatcg gggccgaagg
     4441 gcgcggtcgc aatcggcgtc gtctccaggc gtccatacgt gcgtcgatcg caacgcagct
     4501 tcgtgaaggc gtccgacgcc gcgtacgcgg tcatcttcgt gtgccatggg ttcgctgccg
     4561 acgagcctgc gcagccgacg gacgcagtgt gatgcaggag ttggtatgcg acgaagtccg
     4621 gatcgcaatc ggcagggact cgcaagacgg tatcgcccgg gtcggcgatt cgtgcgatct
     4681 gccgcagcgc atcgtcgctc gtcggtgtcg tttgcatatc gacggacggc aacgggatca
     4741 ggaggttcgt gcccagcaac agcaccgacg ccgcgacgac gaacggcgcc cgctgtggcg
     4801 cgcgtgccag gagtcgctcg agcacgatca cagtcgccgc gacaccgact gcgacgagcg
     4861 cgtacgaggc gcgcgctggt gcccgcagcg cgccgagccc cgggatctgc gacagcaggc
     4921 ggaacggcag ccacgaaacg ggctggtcat cgtcgcgcca caggaagcgg ccgccaacct
     4981 tgagcgacgg accgagcgcg agcacgaaca ccgccgccgc cgcgacggcc aagggcaggc
     5041 gacgccggtc gcgtacgaac atgaacgtga cgaagccacc gagcacgagg aagccgggaa
     5101 acagcgtcga ctcgagcgcg tgcgtcgggt cgggcaaccg accgatgctg tcgtcgacga
     5161 cggtcggtcg cggcagcaac accgtcgaac gcgttggttg cgccaccaac gacagtgcgt
     5221 ctgccgagta caagttgctc tcggcgagca gcgcgtcgtc gtcatgtcgc gtgtcctcgc
     5281 ggtcgaacgt cagtcggggc acgacgaagg gcgtcaggag cacgaaggcc gcggccgccg
     5341 agagcgcggt catcgcgatc ggtcgccact cgtggtctcg caccgcgccg acgaagacga
     5401 ttgccgcgta tacgactgcg ccgaagacga ggaagtagat gctgcacagg taggcggcga
     5461 cgaacaggag tgccaagcgc accgggcgca cgggcgaacg acgcgtcaca acgtcgagcg
     5521 cttcgccgac gaacaacggc agcgtccaag cccagagaag tggcggcaca ccgacttgcg
     5581 cgttcagcgc aagcgcgggc gcggtggcga acgcgaccgc gccgatcatc acgaccgccc
     5641 ggttgccggt cagccgccgg gcgagcgccc gcgccgccaa gaggttcagc gccactcccg
     5701 tgatgagagt gaggttgtac gcgagcacgg gtcccgtcac gaggttccac agcgcgcaca
     5761 cgtacgtggg gaggtagccg tcgagcaggc gggcatcgag cccgaacggg gcgatcacat
     5821 cggggatgtg cgcgggcaac acgtggccgt cggcgatcag gcgactcacg cgccagccca
     5881 gccacgcgta gtagcgcgcg tcatcgctgc cgccgagcat ctcgtcacgg gaccgtgtca
     5941 gtgccggcca ggtcgtgcat acgacgacga tcgtgtagag cgcggtctcg agcacgacag
     6001 cgtgcccgcg gttgcccgct cttgccggcg ccgcgtcgcg aaccacgacg ccgctacgca
     6061 gccgggaccg gccggcgccg ggcacggcgg gcgagcccga tgatcggccg ctcgacgacg
     6121 aaccagctga gcgtcgccgc gccgaccacc acgggaaggc cgagcgccaa cagcgcgagc
     6181 cagttccccg agttgacgtc ccaacccagc cagtcgtgct gcacgatgcc gagcacccac
     6241 cagtgccaca agtacaaccc gtagctgacg acgccgaggt acacgagcac cggcgtctgc
     6301 aacgctcgcc ggatgccacg ggtgcgctgg cggcccaggg cggcggggac cacgatgcag
     6361 aagccgattg ccgtctgcaa caaattcagc tcgacgatct gcgcggacga cagcctctcg
     6421 ccggggtcga tgcccgcgag gaacgggatc gcgagcagtg cagcgagggc gccgcaccac
     6481 cagatccacg accgctcgcc ggcgccggcg aaacgctcgg ccaccgcggc cggccagtcg
     6541 tgggtgacgc acacggccaa caagatgccg agcccgaacg gcagggcgtg ttgcggaaac
     6601 actgtcatcc acaccgggtc gaacccggct gcgacggcga cgatcgcgag cacgccgacg
     6661 ccggcaaaca gcccggcacc gacgaactcc gcgacgatcg gccgcgagcg acgcgcgccg
     6721 gcggcgacgc ccaatgcgta caggggcaac aagacgtaga aggtcacttc caccaccagg
     6781 ctccatgccg gcggcagtcc gaccaggaac gggttcttct ccgccgtgta cgtctgcgtc
     6841 aacgagaagt gccgcaggaa gccatcgaag ccgaagatct gcgccttggc gatcaccaac
     6901 gtgaagaacg cgaccacgac ccagtacgcg ggatagatgc gcgccgcacg gcgcgccgcg
     6961 tacctcccgg tgtcgacggc gggcgccttg cccaggtggt gcgcagcgaa cggcaggtac
     7021 aacaggaagc ctgatgtcac aaagaacacc cacacgccgg cgttgagatt cgcccacacc
     7081 gagccgcccc atatgtcgaa gaacaggctc gtgaagaaca tcgagtggta cacgatgatc
     7141 gacagcgccg cgatcgcgcg gatgccttcg aaacatggga agcgctggtg gccctgatgg
     7201 atgccgctca ttctcgcgca tccaaccaac cgaggaacca cttccagacg gccacggcgc
     7261 cgtcgtcggt gaggtgtaca ccgtcggctt cccaaaggtc tgcaccgtcg agcgcttcgg
     7321 cccgcgttcc gcccgggcac accacgtcgc gccacggcac gacgaccacg cgcgagttgg
     7381 cggctgcgag ctcgtcgatg atgacgttga tcgcgtcgac gcgttggggc tgcgcccgct
     7441 cgggcaacgg gtacgccggg tctccgtcgc cgtagcacgg cgtctggaac acgaatacgg
     7501 tgcgcccgtc gttcgtgagc agatcgaacg cgcgctcgta cgacgagcgc accagctcgg
     7561 tccactcggc cgtgccgtac cgaactgttt tgccgtcgac gcggtggtcg aggacttccc
     7621 acgcaccagt gtgaatcacg cgcaccgcat ccggttcggc tgcgatcgtg atccgccacc
     7681 ggtcgtcgat gccgtcgcac acctgcggac gctcgatgat gcgcccggcg gcgtattggt
     7741 cggtttcgac gagcccgcag ccgagcatcg cctggccgcc gacgccgctg atgccctgca
     7801 gttcgccgag gtgacccgcg gccgacagcc cgaagacgag gcccgtcgaa tctccgtaga
     7861 tgtcgaactt gcgctgtggc gcggattcgg tgccgtccgt cgtcggagcc gtgacggaga
     7921 cggccgcgag ggaggtaggt gggagcggtc gtgggcgtgc cgcggtctgt accaacacga
     7981 acgtcaacgc gatcagaccg acgaacgccg cgatcgctcc ccggctgccc gtgcggcgcg
     8041 cgatgcgtcc ggtgcggaac ggcctctcga cgaggcggta tgacgcctcc gagagcacga
     8101 cgaggatcgt cagccgcacg aggaacaacg ccgtgccgtg caggtgcacc ttttcgccgg
     8161 tgacgaacac gcgcaccggc cagtgccaga ggtagatgga atacgaccgc aggccgatcg
     8221 cgaccagcgg tccgagcccg aggaatcggg acaacggcgt cgacgggaac cgcacgatga
     8281 ccgcgatgac gactgcgctc gcgacggaga accagaagaa cccgctgcgg tagagaaaga
     8341 gggtgttctc cgccgcgatc cgcatcgtca ccaccacgcc ggccaacgcg acgatcccga
     8401 tgacgttcgc
//
LOCUS       DASUGA010000333         2828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_280155, whole genome shotgun sequence.
ACCESSION   DASUGA010000333 DASUGA010000000
VERSION     DASUGA010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280155"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VFX21_06615"
     CDS             <1..534
                     /locus_tag="VFX21_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5095662.1"
                     /translation="VPHDHVLGDVNEGWTVAITTLMNERVAIGSGGAGGRSGGVDDLI
                     DFARDHGVNHDPRTRQGLADLYTKTRIQKFLSRRMLTAASQGKVPGPEGSIGKLFGAR
                     VLTQMGELALSIAGPAGVARVDDGYRWNQTMLGAPASHIAGGSDQVLMNIIGERVLGL
                     PGEPRPDKGVAWKSVPR"
     gene            complement(520..1236)
                     /locus_tag="VFX21_06620"
     CDS             complement(520..1236)
                     /locus_tag="VFX21_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEX5095663.1"
                     /translation="MQILRLWRYPVKSLQGEQLGEADVTPDGLAGDRRYGIVDVARGT
                     VLTGRREPRLLFASASLSADGALTITLPDGSVARDDDALSSWLGKRVQLRAASPDFAA
                     KYETQLVIEDDPNAEWFAWEGPAGRFHDSTRTVVSLVTTTTMRDWDERRFRANVVLEG
                     DGEDELVGTTVRLGSVELEITKPIDRCVMVTREQPEGIARELDVLKTIHAQRAGNLAI
                     GALVTTPGRIAVGDELLPRD"
     gene            complement(1429..>2828)
                     /locus_tag="VFX21_06625"
     CDS             complement(1429..>2828)
                     /locus_tag="VFX21_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX5095664.1"
                     /translation="GIVTALEGEPLPSGDIASRLGAFTDISDMEALPDTVDSKIIEVL
                     LGSKNDAHNNYQDFQAFLEAGGKIGLQHDPLLYGAYLLNPFLVRVEMVPMLVVNQGEV
                     AVVKAYVGLPTLDTSGAEFKFGSIVRPGHRGIWAEPLRTGKYPINPRVYAAEIVPTSI
                     LTLNWAEAVSTAHNLDAHLSPIEAKSREGFIFRIDLQVQIHVPDTRAPKVISMVGTMQ
                     NLVNEVLQAAVGNHFRNTIQSLEAVKFVETRQEVQASAFQAITAYLSAYDVETRGVYI
                     QDVEFPQELVEVLTKREIANQEKATFAEQERAEAARVQMEKARGTADMQAQLAQSAVG
                     IDIKNNEAQAREAQARGEAAFVELTGKAEASKTEAIGLAEAKAVEALGLARAEGFKAQ
                     TEALGQMPTALVAVANAVSEGKITVVPEVLVTGGGGGALDGLAATLMRTFGNGHNGHN
                     GNSAAPKPELEPDKS"
BASE COUNT          453 a          975 c          925 g          475 t
ORIGIN      
        1 gtgccgcacg accacgtgct cggcgacgtc aacgaaggct ggacggtcgc gatcacgacg
       61 ctgatgaacg agcgcgtcgc gatcggctcc ggcggcgcgg gtggtcgcag cggcggtgtc
      121 gacgacttga tcgacttcgc acgggaccac ggcgtcaacc acgatccgcg gacacgtcaa
      181 gggctcgccg atctctacac gaagacgcgc atccagaagt tcttgagcag gcgcatgctc
      241 acggccgcgt cgcagggcaa ggtccccggt cccgaaggat cgatcggcaa gctcttcggc
      301 gcgcgggtgc tcacgcagat gggcgagctc gccctttcga tcgcgggccc ggccggcgtc
      361 gcgcgcgtcg acgacggcta ccgctggaac cagacgatgc tcggcgcgcc cgcgtcgcac
      421 atcgcaggcg gttccgacca ggtgctgatg aacatcatcg gcgaacgggt gctgggtctc
      481 cccggcgagc cgcgccccga caagggcgtc gcctggaaat cagtcccgcg gtagcaactc
      541 gtcgccgacc gcgatgcggc cgggcgtcgt gaccagcgcg ccgatcgcga ggttgccggc
      601 ccgttgcgcg tgaatggtct tgagcacgtc gagctcgcgc gcgatgccct cgggttgctc
      661 gcgcgtcacc atcacgcagc ggtcgatcgg cttcgtgatc tcgagctcga ccgaaccgag
      721 ccgcacggtc gtgccgacga gctcgtcctc accgtcgccc tcgagcacga cgttggcgcg
      781 gaagcggcgc tcgtcccagt cgcgcatcgt cgtggtcgtc acgagcgaga ccaccgtgcg
      841 agtcgagtcg tggaaacgcc ccgcgggccc ttcccacgcg aaccattccg cgttcggatc
      901 gtcctcgatc acgagttggg tctcgtactt cgccgcgaag tcgggtgacg cggcacgcaa
      961 ctggacgcgc ttgccgagcc acgacgacag cgcgtcgtcg tcgcgcgcga ccgaaccgtc
     1021 gggaagcgtg atcgtgagcg cgccgtcggc ggaaagcgac gccgacgcga acagcagccg
     1081 tggttcgcgg cgcccggtca gcaccgtgcc gcgggcgacg tcgacgatgc cgtagcgacg
     1141 gtcaccagcc agaccgtccg gcgtgacgtc ggcttcgccc agttgctccc cctggagcga
     1201 cttgaccggg tagcgccaca aacgaaggat ctgcactggc gaaagacact agtggtgttc
     1261 tccggaattg agtcagccat tccacacacg cgtcgacgga cgcatgatcc gaattcctag
     1321 gaagggaatc cgcggccagc cgatgccctc gtgcgaacga cggcgaaaga agagttcggc
     1381 ccggacgccg gtgggcgtcc gggccgttct gtgttcgcga gtcgtgcgtt acgacttgtc
     1441 gggctccaac tccggcttcg gcgcggcgct gttgccgttg tggccgttgt ggccgttccc
     1501 gaacgtgcgc atgagcgtcg ccgcgaggcc gtcgagcgca ccgccaccgc cgccggtgac
     1561 gagcacctcg ggcacaacgg tgatcttgcc ctccgacacc gcgttcgcga ccgcgacgag
     1621 cgcggtcggc atctgaccga gcgcttcggt ctgcgccttg aaaccttcgg cgcgagcgag
     1681 acccaacgct tcgaccgcct ttgcctcggc cagaccgatc gcctcggtct tcgacgcctc
     1741 ggccttaccg gtcagctcga cgaacgccgc ctcaccacga gcctgcgcct cgcgtgcttg
     1801 tgcttcgttg ttcttgatgt cgatcccgac cgccgactgc gccagctggg cctgcatgtc
     1861 cgcggtaccg cgggccttct ccatctgcac ccgggcggcc tcggcacgct cctgctccgc
     1921 aaacgtcgcc ttctcctggt tcgcgatctc ccgtttggtc agcacctcga ccagctcctg
     1981 cgggaactcg acgtcctgga tgtacacacc gcgcgtctcg acgtcgtagg ccgagaggta
     2041 cgcagtgatc gcctggaacg cgctcgcctg cacctcttgg cgggtctcga cgaacttcac
     2101 cgcctccaag ctctggatcg tgttgcggaa gtggttgccg acggccgcct gcaagacctc
     2161 gttgacgagg ttctgcatcg tgccgaccat cgagatcacc ttcggcgccc gcgtgtcggg
     2221 gacgtggatc tgcacctgca agtcgatccg gaagatgaag ccctcgcggc tcttcgcttc
     2281 gatcggcgac aagtgcgcgt ccaggttgtg cgcagtcgac accgcctcgg cccagttcag
     2341 ggtcaagatc gacgtcggca cgatctccgc cgcgtacaca cgcgggttga tcgggtactt
     2401 gcccgtgcgc aacggctcgg cccagatacc gcggtgaccc ggacgcacga tcgaaccgaa
     2461 cttgaactcg gcaccggagg tgtcgagcgt gggcagaccg acgtacgcct tgacgaccgc
     2521 gacctcacct tggttgacga cgagcatcgg gaccatctcg acgcgcacca ggaacgggtt
     2581 gagcaagtac gcgccgtaca gcaacgggtc gtgctgcaga ccgatcttgc cgccggcctc
     2641 gaggaacgcc tggaagtcct ggtagttgtt gtgggcgtca ttcttcgacc cgagcaacac
     2701 ctcgatgatc ttcgagtcga cggtgtccgg gagcgcttcc atgtccgaga tgtcggtgaa
     2761 ggcaccgagg cggctcgcga tgtcacccga cggcaacggc tcgccctcga gcgcggtcac
     2821 gatgccga
//
LOCUS       DASUGA010000334         3371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_228114, whole genome shotgun sequence.
ACCESSION   DASUGA010000334 DASUGA010000000
VERSION     DASUGA010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228114"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VFX21_06630"
     CDS             <1..694
                     /locus_tag="VFX21_06630"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX5095665.1"
                     /translation="VFTQSEMDAVFREIDDSLPDYTPDDGHSWWARTAKGEHRAVRLD
                     SFDEHSPTTHRIIRDGRLDVIRDLFDDGHQSGDREIRGNVIEALIKPVGVVEGISDVP
                     WHKDCSLGRHSYRCCAMTVGISVTGADATCGQLRVVAGSHRALIQPAFVRKQLDLPQI
                     DLPTETGDVTVHLSCTMHMSEPPVDSERRVMYTGFGLPDPNHDSRIGDAKLRAIRNRA
                     HTTVSQEPGYIS"
     gene            704..1687
                     /locus_tag="VFX21_06635"
     CDS             704..1687
                     /locus_tag="VFX21_06635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX5095666.1"
                     /translation="MIRIGLQIPNFTYPGVAPVDLFDRIAGIARTAEESGFDTVMVMD
                     HFYQLPTLGPPEHEMFEAYTLLGAIAARTERAQLGTLVTGVTYRNPTLLAKAVTALDV
                     ISRGRAFLGIGAAWFDFEHNALGFDFPSTGTRMEMLDEAVQICRAMFRGEHPTFEGKH
                     YRTVNAINSPAPIQAGGPPIMIGGQGEKKTLKLVAKHADMWNCTAAFDELPHKLDVLA
                     QHCANVGRDIKEINKTPLGSIVVADTHEAAVAKRTDFLAARGIDWDALPDEIRKGLEA
                     RIVLGDPDEVGERVRAILDTGMDGVVFNMPADGWDLEAVAQLGEVLTKACN"
     gene            1858..>3371
                     /locus_tag="VFX21_06640"
     CDS             1858..>3371
                     /locus_tag="VFX21_06640"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX5095667.1"
                     /translation="MGKRFVAVALAALLLGGACSGDDDDSASEAAGATREPAPAITGP
                     AAFTTNGSINAAWVTDADPGTALMLVDGEGMKVAQQSADDAGSAIFYDVAPGDGYTVR
                     ETESDEVLGSDEFAVLEPGATPKATLYDQPLEPGLNYVKMRDGVELAMTLRLPTGKTM
                     DDAPFATVIEYSGYQVAAPHDLLNDVASRLGDPDLPADPLVPSTSTAVGSLIAPAIGF
                     ATVSVQMRGTGCSGGAFDLFDYPTVYDGYDAVETVATQDWVKGGKVGMVGISFSGMTQ
                     LFTGGTQPPHLAAVAPLSVTDDLYRTVGSPGGIYNKGFAQSWLEERTEDAKPAPEGGQ
                     PWAKELVAQGDEHCIANQKLHSQARDVFEILSENPERVPELYDHRAPAKWGADIDVPI
                     FMASAFQDEQIVGVGPRLVSGLEDNPNVYITLMNGTHVDPLGPGTITRWAEFLGLFVG
                     DEAITIPDTVLTLGGALYQQIASAAAAPVEQSRFAGRTDVDAVKQEYRQDPRVRV"
BASE COUNT          593 a         1215 c         1081 g          482 t
ORIGIN      
        1 cgtcttcacc cagtccgaga tggacgccgt gttccgcgag atcgacgact cgctccccga
       61 ctacacgccg gacgacggcc attcgtggtg ggcgcgcacc gcgaagggcg agcaccgcgc
      121 cgtccggctc gactccttcg acgagcactc gccgacgacg caccgcatca tccgcgacgg
      181 tcgcctcgac gtgatccgcg acctgttcga cgacggccac cagagcggcg accgcgagat
      241 ccgcggcaac gtcatcgaag cactcatcaa accggtcggc gtcgtcgaag gcatctccga
      301 cgtgccgtgg cacaaggact gcagcctcgg aaggcactcg taccgctgtt gtgcgatgac
      361 cgtcggcatc tcggtgaccg gcgccgacgc gacgtgcggg cagctgcgcg tcgtcgccgg
      421 ttcacaccgt gcgctgatcc aacccgcgtt cgtccgcaaa cagctcgacc tgccgcagat
      481 cgacctgccg accgagacgg gcgacgtcac cgtgcacctg agctgcacga tgcacatgag
      541 tgaaccgccg gtcgacagcg aacgacgcgt catgtacacg gggttcgggc tccccgaccc
      601 caatcacgac tcgcgcatcg gcgacgccaa gctgcgcgcg atccgcaacc gcgcccacac
      661 gacggtgtcg caggaaccgg gctacatctc gtaaggaccc cgcatgattc gcattggcct
      721 tcagatcccg aacttcacgt atcccggcgt cgcgccggtc gacctgttcg accgcatcgc
      781 cggcatcgct cgcaccgccg aagagtccgg gttcgacacc gtcatggtga tggaccactt
      841 ctaccaactg ccgacgctcg ggccgccgga gcacgagatg ttcgaggcct acacgttgct
      901 cggcgcgatc gcggcgcgta ccgaacgggc ccaactcggc acgctcgtca cgggtgtcac
      961 ctaccgcaat cccacgctgc tcgccaaggc ggtgaccgcg ctcgacgtga tctcacgcgg
     1021 ccgcgccttc ctcggcatcg gcgccgcgtg gttcgacttc gagcacaacg cactcggctt
     1081 cgacttcccc tcgaccggca ctcgcatgga gatgctcgac gaagcagtgc agatctgccg
     1141 cgcgatgttc cgcggcgagc acccgacctt cgagggcaag cactaccgca ccgtcaacgc
     1201 gatcaactcg cccgcgccca tccaagccgg cggcccaccg atcatgatcg gcggccaggg
     1261 cgagaagaag acgttgaagc tcgtcgcgaa gcacgccgac atgtggaact gcaccgcagc
     1321 cttcgacgag ctcccgcaca agctcgacgt gctcgcgcag cactgcgcga acgtcggtcg
     1381 cgacatcaag gagatcaaca agacaccgct gggatcgatc gtcgtcgccg acacgcacga
     1441 ggctgcggtc gccaagcgca ccgacttcct cgccgcccgc ggcatcgact gggacgcgct
     1501 gcccgacgag atccgcaaag gtctcgaagc gcgcatcgtg ctcggtgacc ccgacgaagt
     1561 cggcgagcgg gtgcgcgcca tcctcgacac gggcatggac ggcgtcgtgt tcaacatgcc
     1621 cgccgacggc tgggacctcg aagccgtcgc ccaactcggc gaagtcctca ccaaagcctg
     1681 caactgactc gggcgtcgat ggcccccaat agttgagggc gagcgacgcc cgtccggatc
     1741 ggttcggcgc ggcactcggt cgaccctcgg catcatcttc cacgacgcga cgtgagtcgc
     1801 ccccgcgcgg cccggtagat tgcgccgact gcacgggcgg cggccggggg acctctgatg
     1861 ggcaaacgat tcgttgcggt cgcgctcgcg gccctcctct tgggcggtgc ctgctcgggt
     1921 gacgacgacg actcggcgtc cgaggccgcc ggcgcaacgc gcgaacccgc acccgcgatc
     1981 accgggcccg cggcgttcac cacgaacggc agcatcaatg cggcgtgggt gaccgacgcc
     2041 gatcccggca cggcgctcat gctcgtcgac ggcgagggaa tgaaggtcgc ccaacagtcc
     2101 gccgacgacg caggcagcgc gatcttctac gacgtcgcgc ccggcgacgg ctacaccgtg
     2161 cgcgagacgg aaagcgacga agtgctcggc agcgacgagt tcgcggtgct cgaaccgggc
     2221 gccacgccca aggcgacgct ctacgaccag ccgctcgagc ccgggttgaa ctacgtcaag
     2281 atgcgcgacg gcgtggagct cgcgatgacg ctgcggctgc cgacgggcaa gacgatggat
     2341 gacgcaccct ttgcaacggt catcgaatac tcgggctatc aggtcgcggc gccgcacgat
     2401 ctcctgaacg acgtcgcgtc acggctcggc gaccctgacc tccccgcgga tccactcgtg
     2461 ccgtcgacgt cgacggcagt cggttcgctc atcgcacccg cgatcggctt cgcgacggtc
     2521 agcgtgcaga tgcgcggcac cggttgttcg ggcggcgcgt tcgacctctt cgactacccc
     2581 accgtctacg acggttacga cgcggtcgag accgtcgcga cccaggactg ggtcaagggc
     2641 ggcaaggtcg gcatggtggg catcagcttc tccggcatga cgcagctgtt cacgggcggg
     2701 acgcagccgc cgcatctcgc cgcggtcgcg ccgctctcgg tcaccgacga tctctaccgc
     2761 accgtcggtt cgcccggcgg catctacaac aaaggcttcg cacagtcgtg gctcgaggag
     2821 cgcaccgagg acgccaagcc cgcgcccgag ggtgggcagc cgtgggcgaa ggagctcgtc
     2881 gcgcaaggtg acgagcactg cattgcgaac cagaagctgc actcgcaggc gcgtgacgtg
     2941 ttcgagatcc tgagcgagaa ccccgagcgc gtgcccgagc tgtacgacca ccgcgcgccc
     3001 gcgaagtggg gcgccgacat cgacgtgccg atcttcatgg ccagcgcgtt ccaagacgaa
     3061 cagatcgtcg gcgtcggtcc gcgcttggtc tccggactcg aggacaaccc caacgtgtac
     3121 atcacgctga tgaacggcac gcacgtcgac ccgctcggac cgggcacgat cacgcgctgg
     3181 gccgagttcc tcgggctgtt cgtcggcgac gaggcgatca cgatccccga caccgtgctc
     3241 acactcggcg gcgcgctcta tcagcagatc gcgagcgcgg ccgccgcacc cgtggagcag
     3301 tcacggttcg cgggccgcac cgacgtcgac gccgtgaagc aggagtaccg ccaggacccg
     3361 cgtgtgcggg t
//
LOCUS       DASUGA010000335         2005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393 k141_2440,
            whole genome shotgun sequence.
ACCESSION   DASUGA010000335 DASUGA010000000
VERSION     DASUGA010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2440"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..1107)
                     /locus_tag="VFX21_06645"
     CDS             complement(190..1107)
                     /locus_tag="VFX21_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HEX5095668.1"
                     /translation="MRRYEATVYDSRRWDGFELRAGDIIICTPPKCGTTWTQMICAQL
                     VLQEPELPLPLDTLSPWIDMVTRARTEVFSDLAAQTHRRFIKTHTPLDGIPNDSRVTY
                     ICVGRDPRDVALSMDHHIDNMDVGAFVEARERAAAIDGIELGPLRPPRGRPDDERARF
                     WRWVDDDTPSTESGSTLRFTVEHVQSFRDAADDLDVVFLHYDDLQADLEGQMRELAAH
                     LGIAVEEDRWPRLVDAATFESMRSRADTMVPGGGREHWIDTTAFFSRGTSGQWRDLLD
                     ASDQARYAARVRALASDDLVEWLHREPVD"
     gene            complement(1163..>2005)
                     /locus_tag="VFX21_06650"
     CDS             complement(1163..>2005)
                     /locus_tag="VFX21_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095669.1"
                     /translation="QINFKGDDRAGRSEIRGGNIGVNNTGFAERGEPRLTMCLGPGDG
                     LNVVMDPHTQVVADTAKLGGACDVWDVFVTKLEASGHTPHTNSVNGGWYPVGAPEPLI
                     TSLPPFPAFTCAPSPHVTFKNTTLPPGNYGNVSIEGSVSLGAGTYTFCDLHVERGARL
                     ATTNATVIQVQGALRVPGGSIGSAQSCDANIFVKADLPRDMNGVAFGRGSEIFGQLWA
                     PNADVALGHSTDLHGHFWAKSMTSDRGVNVTGCGTTAATTTSTSTTTTAPETSTTETT
                     LPDP"
BASE COUNT          290 a          700 c          695 g          320 t
ORIGIN      
        1 accgacgacg tgcgcaaggc ctacatcgtc cagtacgcgc ccgacggtgc cgtcgcgtac
       61 cgaccgcaac ccgacggcag ccgcgggacc gccgaaccgc tcgacgacga gcgccgccaa
      121 ttcctcgtcg tgcgcgacgg cgagccagtc cgcgagcgcc cgtagcaacc acgagccgcg
      181 accgtcggct cagtcgaccg gctcgcggtg caaccattcg acgaggtcgt cggacgcgag
      241 cgcccgcacg cgcgcggcgt accgcgcctg gtcggacgcg tcgaggaggt cgcgccactg
      301 gccgctcgtt ccgcggctga agaacgctgt ggtgtcgatc cagtgctcac gtccaccacc
      361 ggggaccatt gtgtcggccc ggctccgcat cgactcgaac gtcgccgcgt cgacgagccg
      421 gggccaacga tcttcctcta ccgcgatgcc gaggtgcgcg gccagctcgc gcatctgccc
      481 ctcgaggtcg gcctgcaggt cgtcgtagtg caggaacacg acgtcgagat cgtcggccgc
      541 gtcccggaac gactgcacgt gctcgacggt gaaccgcagc gtcgacccgg actccgtcga
      601 cggcgtgtcg tcgtcgaccc agcgccagaa ccgagcccgc tcgtcgtcgg ggcgcccccg
      661 cggtgggcgc aacggcccga gctcgatgcc gtcgatcgcg gccgcccgct cgcgggcctc
      721 gacgaatgcg ccaacgtcca tgttgtcgat gtggtggtcc atcgagagcg ccacgtcacg
      781 cgggtcgcgg ccgacgcaga tgtacgtgac cctcgaatcg ttggggatcc catcgagcgg
      841 cgtgtgggtc ttgatgaaac ggcggtgcgt ctgcgccgcc aagtcggaga acacctcggt
      901 gcgggcacgg gtcaccatgt cgatccacgg tgagagcgtg tccaacggga gcggcagctc
      961 cggttcctgc aggacgagct gcgcgcagat catctgcgtc catgtcgtcc cgcacttggg
     1021 cggcgtgcag atgatgatgt cgccggcgcg gagctcgaac ccgtcccagc gacggctgtc
     1081 gtagacggtt gcctcgtagc gtcgcacggc ggtcatcctc ccaccgaccc gacagtgccg
     1141 gatgccgcac gtccggaacg ggctacgggt ccgggagggt cgtctccgtc gtcgacgttt
     1201 cgggcgccgt cgtggtcgtc gacgtgctcg tcgtcgtggc ggccgtcgtg ccgcatcccg
     1261 tcacgttgac accgcggtcg ctcgtcatcg acttcgccca gaagtgcccg tgcaggtcgg
     1321 tgctgtggcc gagggcgacg tccgcgttcg gcgcccagag ctgcccgaag atctccgagc
     1381 cccgcccgaa ggcgacaccg ttcatgtcgc gcggcagatc ggccttcacg aagatgttgg
     1441 cgtcgcacga ctgtgcgctg ccgatcgagc cgcccggcac gcgcagcgcg ccttgcactt
     1501 ggatcacggt ggcgttcgtc gtggcgagcc gtgcgccccg ctcgacgtgc aggtcgcaga
     1561 acgtgtaggt gcccgcgccg agcgacaccg acccttcgat gctgacgttg ccgtagttgc
     1621 cgggcggcaa tgtcgtgttc ttgaaggtca cgtgcggtga cggcgcgcac gtgaacgcgg
     1681 gaaacggcgg cagcgacgtg atgagcggct cgggcgcgcc gaccgggtac cagccaccgt
     1741 tgaccgagtt cgtgtgcggc gtgtgtcccg acgcctcgag cttggtgacg aagacgtccc
     1801 agacgtcgca cgcgccgccg agcttcgcgg tgtctgcgac gacttgggtg tgcggatcca
     1861 tgacgacgtt cagcccgtcg ccgggcccga ggcacatcgt caggcgcggc tcgccccgct
     1921 ccgcgaagcc ggtgttgttc acaccgatgt tgccgccgcg aatctcggag cgcccggcgc
     1981 gatcgtcgcc cttgaagttg atctg
//
LOCUS       DASUGA010000336         2396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1957174, whole genome shotgun sequence.
ACCESSION   DASUGA010000336 DASUGA010000000
VERSION     DASUGA010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1957174"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VFX21_06655"
     CDS             complement(<1..323)
                     /locus_tag="VFX21_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015488960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5095670.1"
                     /translation="MGANTSFGRYRDIDAAELAARLGTADSPFVVDVREPNEFAEWSI
                     PTAVNVPLSELPSRIDSLPRDREVVVMCAAGGRSARAAEALVDAGLHVANLRGGMSAW
                     GAVYD"
     gene            complement(384..1919)
                     /locus_tag="VFX21_06660"
     CDS             complement(384..1919)
                     /locus_tag="VFX21_06660"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX5095671.1"
                     /translation="MSQPAGTLTGGRLAGAFIALMVVNTMSGVDTTIVSTAAPTITSD
                     LGRLSLLPWISTAYLLAQIGTIALYGKLGDLYGRKRVFLTAASLFLVASVLCGAAQSM
                     PQLVAFRFLQGAGAGGLTGLAMAIVADIAPPSRVGRYLGYAGLVYAFTSVLGPLTGGL
                     FVDHLSWRWAFYINLVPGLIGGAFVIALVPSEQKERIRHRIDVLGAILLAATTTALVL
                     TTSWGGSEYEWTSPVIVFLVIAVVGAGAAFVFREQRAPEPLVPLGVLRERTVSLSIGA
                     NLVAGAAFTAGIVFLPILYQAVIGRDATSSGLLLIPLGLATALSTVVAGQVVDRYGGV
                     KVIPATGMVTMALAYAALSTVDADTSAAFGATCALFVGIGVGCVMQTLLHVVQRSVPI
                     ENLGIATSSVMLGRIMGSALGVALFGAIFNARLEPALAAIPGVVPDDVHGDPASIRQL
                     GAGLRHAVAAAFADALAGGFTALVGVSLLGLGTVLALRGRVLRRRIAIPVDLDAQVAP
                     TAP"
     gene            complement(1916..>2396)
                     /locus_tag="VFX21_06665"
     CDS             complement(1916..>2396)
                     /locus_tag="VFX21_06665"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEX5095672.1"
                     /translation="ESNSVTSSGIDITGVVVPVDIPAACGPIPASHPHVTLLAPFRAR
                     PRLDAALCSELAAFFATQRAFTMEFVAERRFVDGPRYLAPEPVDRIVAMTDALAVRYP
                     DTPPYEGQFRDIVPHCTLDLDGPAPRLPIAMPVTEAHLVHSFGDTWDVIARFPFANP"
BASE COUNT          416 a          809 c          875 g          296 t
ORIGIN      
        1 cagtcgtata ccgcgcccca cgcgctcatg ccgccgcgca ggttcgcgac atgcagaccc
       61 gcgtccacga gcgcttcggc ggcgcgtgcg ctacggccgc cggccgcgca catgacgacc
      121 acctcacggt cgcggggcaa tgaatcgatc cgtgacggga gctccgagag cggcacgttg
      181 accgcggtcg gaatcgacca ctcggcgaac tcgttcggtt cgcgtacgtc gaccacgaac
      241 ggcgaatcgg cggtgccgag ccgcgccgcg agttccgcgg cgtcgatgtc gcgataccga
      301 ccgaacgatg tgttcgcgcc catggcggct ctccttccgg ggtggtttcg ggtcccacca
      361 ctataccccc gggggtattc cgcctacggc gcggtgggtg cgacctgcgc atcgaggtcg
      421 accgggatcg cgatgcggcg gcgcaggacg cggccgcgca acgcgagcac ggtgccgagc
      481 ccgagcagtg agaccccgac cagtgcggtg aacccgcccg cgagcgcgtc ggcgaacgcg
      541 gcggcgacgg catgacgcag gccggcgccg agctggcgga tcgacgccgg gtcgccgtgg
      601 acgtcgtcgg ggacgacgcc ggggatggcc gcgagcgcgg gttcgagccg cgcgttgaag
      661 atcgcaccga acagcgcgac gccgagcgca ctccccatga tgcggccgag catgacactc
      721 gaggtcgcaa taccgaggtt ctcgatcggg accgatcgtt gcacgacgtg cagcagcgtc
      781 tgcatgacac aaccgacgcc gataccgacg aacaacgcgc acgtcgcgcc gaatgcagcg
      841 gaggtgtcgg cgtcgaccgt gctcagcgcg gcgtacgcga gcgccatcgt caccatgccg
      901 gtcgcgggaa tgaccttgac gccgccgtag cggtcgacca cttggcccgc gaccacggtc
      961 gacagcgccg tcgcgaggcc gagtgggatc aacagcaggc cggacgaggt cgcgtcgcgc
     1021 ccgatgacgg cttggtagag gatcgggaga aacacgatgc ccgcagtgaa cgccgcgccg
     1081 gcgacgaggt tggcgccgat cgacagggag accgtgcgtt ccctcagcac gccgagcggc
     1141 accaacggtt ccggcgcgcg ctgctcccgg aagacgaacg cggcgcccgc gccgacgacc
     1201 gcgatgacga ggaagacgat cacgggcgac gtccactcgt actcgctgcc gccccacgag
     1261 gtcgtgagca cgagcgcggt cgtggttgcc gcgagcaaga tcgcgccgag cacgtcgatc
     1321 cggtggcgga tgcgttcctt ctgctccgag ggcacgagcg cgatgacgaa cgcgccgccg
     1381 atgaggccgg gcacgaggtt gatgtagaac gcccaccgcc acgagaggtg gtcgacgaag
     1441 agtccgcccg tcagcgggcc caacaccgaa gtgaacgcgt agaccagccc cgcgtaaccg
     1501 aggtagcggc cgacgcgcga cggcggcgcg atgtcggcga cgatcgccat cgcgagaccg
     1561 gtgagcccgc ccgcgccggc gccctggagg aagcggaatg cgacgagctg cggcatcgat
     1621 tgcgcggcgc cgcagagcac ggacgcgacg aggaacaacg acgcggccgt gaggaacacg
     1681 cgcttgcgtc cgtagagatc gccgagcttg ccgtaaagcg cgatcgtccc gatctgcgcg
     1741 aggaggtaag cagtcgagat ccacggcagc aacgagaggc gaccgagatc gctcgtgatc
     1801 gtgggcgccg cggtcgagac gatcgtcgtg tccactccgg acatcgtgtt gacgaccatc
     1861 agcgcgatga acgcgcccgc gagccggccg ccggtgagcg tgcccgccgg ttggctcatg
     1921 ggtttgcgaa cgggaagcgc gcaatgacgt cccacgtgtc gccgaacgag tgcacgaggt
     1981 gcgcctcggt gaccggcatc gcgatcggca gccgcggcgc cggcccgtcg agatccaagg
     2041 tgcagtgggg gacgatgtct cggaactgcc cctcgtaggg cggggtgtcg gggtagcgca
     2101 ccgcgagcgc gtcggtcatc gcgacgatgc ggtcgacggg ctccggcgcg aggtaacgcg
     2161 gcccgtcgac gaaccggcgc tcggcgacga actccatcgt gaacgcgcgc tgcgtggcga
     2221 agaacgcggc cagctcgctg cacagcgcgg cgtcgaggcg gggccgggcc cggaacggcg
     2281 ccagcagtgt cacgtgagga tgcgaggcgg ggatcggccc gcaggccgcg gggatgtcca
     2341 ccggcaccac gacccccgtg atgtcgatgc cgctactcgt caccgaattc gactcc
//
LOCUS       DASUGA010000337         6654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_469557, whole genome shotgun sequence.
ACCESSION   DASUGA010000337 DASUGA010000000
VERSION     DASUGA010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6654
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469557"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFX21_06670"
     CDS             complement(<1..196)
                     /locus_tag="VFX21_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEX5095673.1"
                     /translation="MTPDVDRLLADLQALAAVGATGDGGNCRLALTDDDAGGRELVVG
                     WMRDLGLDVRVDRIGNVVATR"
     gene            249..755
                     /locus_tag="VFX21_06675"
     CDS             249..755
                     /locus_tag="VFX21_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEX5095674.1"
                     /translation="MHPTDATTDDIREELSWELFGDATRELAEVIADDGFVPDVVLAV
                     ARGGLTVAGALAYALGVKNCGAVNVEFYTGVDERLDVPVLLPPTLNLLEVADMRVLVA
                     DDVADTGHTLELVRKVLDEHVGELRTAVLYHKPRSVILPTYSWRTTSLWINFPWSTLP
                     PVAGLPPR"
     gene            843..1745
                     /locus_tag="VFX21_06680"
     CDS             843..1745
                     /locus_tag="VFX21_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5095675.1"
                     /translation="MESFELRYATIHGHDVGYRTGGSGPVLLLVHGMAGSSDTWLPVL
                     PALAERFTVVAPDLVGHGASAKPRGDYSLGAFANSLRDLLVTLGHERVTVVGQSLGGG
                     VVMQFAYQFPERCERIVLVSSGGLGEEVNLLLRALALPGAEYVLPVACTTWLHDRVVS
                     VFRGLRRVGLRPSPNLVEVWRSYGSLADAPTRTAFVHTLRSVIDVAGQRVSARDRLYL
                     ASALPTLIIWGDHDRIIPVGQAYATHEAIPGSRLEIFEGVGHFPHCERPRHFVEVLTA
                     FMHSTEPGPVSAEHWQQAILAQQG"
     gene            complement(1789..2139)
                     /locus_tag="VFX21_06685"
     CDS             complement(1789..2139)
                     /locus_tag="VFX21_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095676.1"
                     /translation="MKTVFTQTITFRCDQPEDLVAAAREWDALQAHQDVMGYMEVRIL
                     ADRDDPGRYLMVSDFGVVHPELSAAQEAFLNNERPQTQQFAERMRALATSEPEWHHYD
                     ELYRTTFGQEDTSE"
     gene            2276..2533
                     /locus_tag="VFX21_06690"
     CDS             2276..2533
                     /locus_tag="VFX21_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX5095677.1"
                     /translation="MPDRRADATADSTLLAIRVIPRARRNEVSGERDGRLVVRTTAAP
                     VDGSANDSVRKLVAQHLGLPVRAVSIASGHHSRDKVLRIAR"
     gene            2586..2984
                     /locus_tag="VFX21_06695"
     CDS             2586..2984
                     /locus_tag="VFX21_06695"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX5095678.1"
                     /translation="MTLVVGDTAAHYFAYDYEPATGTVGPRRIFGDMSDLPGGPDGAT
                     LDADEGLWCALFGGAAVVRFTNAGLDRTIEVPVTNPTDVTFGGRDLDRLYLVSVADPN
                     AVVGALDGALLVIDGLGTRGRPEPRFAAVR"
     gene            complement(3061..4176)
                     /locus_tag="VFX21_06700"
     CDS             complement(3061..4176)
                     /locus_tag="VFX21_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008733030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX5095679.1"
                     /translation="MPLPPDPPLDLAPALQRRLSQADQAVGRLDGLTRFLPDVDLFLA
                     MFVRREALLSSQIEGTECTLDDVISYELDEASAVRSLDVREVVNYVAAMEDGLRGLDH
                     LPLSNRLLRDVHGTLLRDGRGADKEPGEFRRSQNWIGPPGSTLTTATYVPPPPHVMQD
                     AMAALERFVHDSDLPILVTAGLAHAQFETIHPFLDGNGRIGRLLVTLLVCERRVLTRP
                     VLYLSTFLRRHRSRYFDILTAIRNDGAWEAWLEFFLDGVATTAQEAADSAERVHELRE
                     RDRRRVMDASGGNNELRLLDALYRQPVVNAAWVEKHLEVASATANKVLERCTTAGVIR
                     ETTGKRRNRVYRYDDYLALFDAPVVPIEDETTVSNES"
     gene            complement(4432..4788)
                     /locus_tag="VFX21_06705"
     CDS             complement(4432..4788)
                     /locus_tag="VFX21_06705"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX5095680.1"
                     /translation="MSTSVVPSPADADALRRSRSALLTELATQRQVLADCDAALEELD
                     AIGDRDAIDTRESVRLAQARALGAVDNIERALIRLRYGTYGRCDECGSTIGAARLEAL
                     PATRHCISCASTTLDA"
     gene            complement(4845..5150)
                     /locus_tag="VFX21_06710"
     CDS             complement(4845..5150)
                     /locus_tag="VFX21_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095681.1"
                     /translation="MVIATVFAALALTVVAAGPDIAPVENGVSSGVDTPDDAVEQPAR
                     PGTRAAALVPLMLLGGLAFVAPPVRRRVALLRRRFRRIGDAGDDWRALLLGAPPVAA"
     gene            complement(5409..5915)
                     /locus_tag="VFX21_06715"
     CDS             complement(5409..5915)
                     /locus_tag="VFX21_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095682.1"
                     /translation="MVQQRERAYPKWTLGIGTGVESSTMALYVTIVLLATTSLLTDAD
                     MHDIGILAVVWGESFGLAIAHFFAFVLAVHLVHGEAAPHEWRTAREQLAGALSVATIC
                     TIPVFLVPESAELDVVRVMLAVVLALSGYAAARANGGSQRRAVIVGAVVLVGGLTIAV
                     MKNVIGGH"
     gene            6027..>6654
                     /locus_tag="VFX21_06720"
     CDS             6027..>6654
                     /locus_tag="VFX21_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095683.1"
                     /translation="MPSARRSFAVIAIVAIALTMLVTGTADAGIVIPRDDATVIDGPT
                     VAQEGPSVVIEWSMPKRFGLPQTNAYVRNSAGWKVTVTTCGSYTNASGVTITGSSWIL
                     YDKGVQLASSTTCKKTITLPHLGFYDVKGTIRTSNGGTYTAQRRIELKDHLIVVAGDS
                     MASGEGNPDVKGDADISWCGSSKLEEQLMPWSCVVQMIDLITNNPSVLV"
BASE COUNT         1048 a         2229 c         2276 g         1101 t
ORIGIN      
        1 cgcgcgtcgc gacgacgttg ccgatccggt cgacgcggac gtcgaggccg aggtcgcgca
       61 tccagcccac gaccagctcg cggccgccgg cgtcgtcgtc ggtgagcgcg agacggcagt
      121 tgcccccgtc gccggttgca cccaccgcgg ccagggcctg caggtcggcg aggagccggt
      181 cgacgtcggg tgtcacgcag gcgattctcc cacgacggtt gcgcgtaatg tggcggggga
      241 ggagcacgat gcatccgact gacgcgacca cagacgacat tcgagaagag ctgtcgtggg
      301 agttgttcgg cgacgcgacg cgcgagctcg ccgaagtgat cgccgacgac ggcttcgttc
      361 ccgatgtcgt gctcgcggtc gcgcgcggcg gtctcacggt cgccggcgcg ctcgcctacg
      421 cgttgggcgt caagaactgc ggtgcggtca acgtcgagtt ctataccggc gtcgacgagc
      481 gcctcgacgt tccggtgctg ctgccgccga cactcaacct cttggaggtt gccgacatgc
      541 gcgtgctcgt cgccgacgac gtcgccgaca ccggccacac gctcgagctc gtgcgcaagg
      601 tcctcgacga gcatgtcggc gagctccgca ccgcggtgct gtaccacaag ccccggtcgg
      661 tgatcctgcc gacctactca tggcgcacga cgagcttgtg gatcaacttc ccgtggtcga
      721 cgctcccgcc cgttgccggg ctcccgccgc gctgacgccg gtccgcgcgt cggacgcact
      781 agcgagatgc agcgtctggt ggtggcgcgc gcgatcagcg ggatgcacat accgtacgcc
      841 gtatggaatc tttcgagctc cggtacgcga cgatccacgg acacgatgtc gggtaccgga
      901 ccggtgggtc cggcccggtc ctgttgctcg tccacggcat ggccggcagc tccgacacct
      961 ggctgccggt gttgcccgcg ctggcggagc ggttcacggt ggtcgccccc gacctcgtgg
     1021 gccacggcgc gtcggcgaag ccgcgcggcg actactcgct cggcgcgttc gccaacagcc
     1081 tgcgcgacct cctcgtgaca ttgggtcacg agcgcgtcac ggtggtgggc cagtcgctcg
     1141 gcggtggcgt ggtgatgcag ttcgcgtacc agttcccgga acgctgcgag cggatcgtgc
     1201 tcgtgtcgag tggtgggctc ggcgaagagg tgaacctctt gctccgcgcg ctcgcattgc
     1261 ccggcgccga gtacgtgctg cccgtcgcgt gcacgacgtg gctccacgac cgggtcgtct
     1321 cggtgttccg cggcctccgg cgcgtcggcc ttcgcccgag tcccaacttg gtggaggtct
     1381 ggcgaagcta cggttcgttg gccgacgcgc cgacccgtac tgcgttcgtg cacacgctgc
     1441 ggtcggtcat cgatgtggcc ggccagcgtg tcagcgcccg cgatcgcttg tatctcgcgt
     1501 cggcgcttcc gacgttgatc atctggggcg accacgaccg catcatcccg gtcgggcagg
     1561 cgtacgcgac gcacgaagcg atccccggca gccggctcga gatcttcgaa ggagtgggcc
     1621 actttccgca ctgcgaacgg ccccgccact tcgtggaggt gctcaccgcg ttcatgcact
     1681 ccaccgaacc cgggcccgtg agcgccgaac actggcagca ggcgatcctc gcgcagcaag
     1741 gctgagcgcg gcgcatcacg ttcccgtcgt tgtatgcctt ggccgcgtct actcggatgt
     1801 gtcttcctgt ccgaaggtcg tgcggtacaa ctcgtcgtag tgatgccact ctggctctga
     1861 tgttgcgagc gcgcgcatcc gctccgcgaa ctgttgcgtc tggggccgct cgttgttgag
     1921 gaacgcctct tgggcggctg acaactcggg gtgcacgacc ccgaagtcgc tgaccatgag
     1981 gtaccggcca gggtcgtcac gatcggcgag gatccgcacc tccatgtagc ccatgacgtc
     2041 ttgatgcgcc tggagcgcgt cccattcccg tgccgcggcg accagatcct cgggttgatc
     2101 gcatcggaac gtgatcgttt gtgtgaagac agttttcatg gcgacgcagg gtgccaagcc
     2161 gaagtcgatc atgcaagaca gagccaccgg acgacatccg gcgcctcgtc gtcaacgccg
     2221 acagttgctc gttcggcgca tgtaagggac aggacggcgc gaggatgggt gacgtatgcc
     2281 cgatcgacgc gccgacgcaa ccgccgactc gaccctgctg gccattcggg tgatcccgcg
     2341 ggcgcgtcgc aacgaggtca gcggcgagcg cgacgggcgc ttggtcgtcc gcacgaccgc
     2401 ggcgccggtc gacggttccg ccaacgactc ggtgcgcaag ctcgtggcgc aacacctcgg
     2461 gctgcccgtc cgcgccgtct cgatcgcgtc cggccaccac tcacgcgaca aggtgttgcg
     2521 catcgcgcgg tagcgtcggc catggatatt tcgcgcgtcg gtgcgtcgcg acgatcggcg
     2581 gcctcatgac gctcgtcgtc ggtgacaccg ccgcccacta cttcgcgtac gactacgagc
     2641 cggcgacggg cacggtcggg ccacggcgca tcttcggtga catgagcgac cttccgggtg
     2701 gtcccgacgg cgccaccctc gatgccgacg aaggactctg gtgcgcgttg ttcggcggcg
     2761 cggccgtcgt gcgcttcacc aatgcgggcc tcgaccggac gatcgaggtg ccggtcacga
     2821 acccgaccga cgtcacgttc ggcggacgcg acctcgatcg cctctatctg gtctcggtcg
     2881 cggaccccaa cgccgtcgtc ggcgcgctcg acggcgcgct gctcgtgatc gacgggctcg
     2941 gcacgcgtgg gcgccccgag ccgcgcttcg ccgccgtgcg ctgacaccgc tgcgcctcgc
     3001 agttgtgaag acttcgccag ggtggtcagg tagtgaacac cgcgccggca cggcttttcg
     3061 ctacgactcg ttcgagactg ttgtttcatc ctcgatcggg acgacgggcg cgtcgaagag
     3121 ggcgaggtag tcgtcgtacc ggtagacgcg gttgcgccgc ttccccgtcg tttcgcggat
     3181 cacgccggcc gtcgtgcatc gttccaacac cttgttcgcg gtggcggacg cgacttcgag
     3241 gtgtttctcg acccaggccg cgttcacgac cggctgccgg tagagcgcgt cgagcaaacg
     3301 cagctcgtta ttgccgccgg acgcgtccat cacacgccgg cggtcccgct cgcgaagttc
     3361 atggacgcgt tcggcggagt ctgcggcctc ctgcgcagtc gtggcaacgc cgtccaagaa
     3421 gaactcgagc catgcttccc atgcgccgtc gttgcgaatc gctgtcagga tgtcgaagta
     3481 ccggcttcgg tgacggcgca agaacgtgct gagatacaag acgggtcgcg tgaggacgcg
     3541 tcgctcacac accagcaacg tcacgagcaa tcggccgatc cgtccgttgc cgtcgaggaa
     3601 tgggtggatg gtttcgaact gagcgtgcgc gagcccggca gtgacgagga tgggaaggtc
     3661 ggagtcgtgg acgaaccgtt cgagcgctgc catcgcgtcc tgcatcacgt gcggtggagg
     3721 cgggacgtag gtcgccgtcg tcagcgtgga tcccggaggg ccgatccagt tttgcgagcg
     3781 ccgaaactcg ccgggctcct tgtcggcacc acggccatcg cgtagcaagg tgccgtgtac
     3841 gtcgcgcagg agccggttgc tcagcggaag gtggtccagt ccgcggaggc cgtcttccat
     3901 cgccgcgacg tagttgacga cttcgcgcac gtcgagcgac cggacggcgc tggcttcatc
     3961 gagctcgtac gagatgacgt cgtcgagtgt gcactccgtt ccttcgattt gtgagctgag
     4021 gagcgcctcg cgtcgaacga acatcgcgag gaacaggtcg acgtccggca ggaatcgggt
     4081 cagtccatcg aggcggccca cggcctggtc ggcctgggag agccttcgct gcaacgcggg
     4141 tgccaggtcg agcggcggat ccggcggcag gggcatgggc tcgaaggcga catagccctc
     4201 gagctgcctg acgaatcgtc ccgcgcggcc ggtactcaca gggtgtccac ggatgagttc
     4261 actatttcag tgttcgacgg atttctgaaa tagtagcggg gtcgtctcgc ccatgggctg
     4321 cattgctgaa ataacggcgt ccgcaacgcg cagctggtac agcgttggca acggaactgt
     4381 gcgcgggcga gccggatatc agggtcagca gctcgcccgc gcacgtgctc gctacgcgtc
     4441 gagggttgtc gacgcgcagc tgatgcagtg ccgagtcgcg ggcagcgctt cgaggcgcgc
     4501 ggccccgatc gtgctgccgc actcgtcgca tcgtccgtag gtgccgtagc gcaggcggat
     4561 cagtgcgcgc tcgatgttgt caacggcacc gagcgcccgc gcctgcgcga gccgcacaga
     4621 ctcgcgggtg tcgatggcat cgcgatcacc gatcgcgtcg agctcctcga gcgcggcgtc
     4681 gcagtcggcc agcacctgac gctgggttgc gagctcggtg aggagcgcac tgcgggagcg
     4741 gcgcagcgca tctgcgtccg cgggtgaagg aacaactgac gtactcatga cgagtccttt
     4801 gctgctcagg tcgcgcgaga cgcgaccgaa tccggtgggc ggcgttacgc cgcgaccgga
     4861 ggcgcgccga gcagcaaggc ccgccagtcg tcgccggcgt cgccgatgcg gcggaaccgg
     4921 cggcggagca atgcgacgcg acgacgaacc gggggagcga cgaacgccaa cccgcccagc
     4981 agcatcaacg gcacgagtgc ggcggcgcgc gtgcccggac gggccggctg ttcgaccgcg
     5041 tcgtccggcg tgtcgacacc cgacgaaaca ccgttctcga cgggcgcaat gtccggcccg
     5101 gcggcgacga ccgtcaacgc gagcgccgcg aacaccgtcg cgatgaccac ggtaaggatg
     5161 cgaagacgtt tcggcacggc gggtccctcg gctgatggtc gatcagtcga aggtctcctc
     5221 cgccgtgagg ccggtgcgcc cgggccggag ccgtgttgac gaacgcaccg cgtgggagta
     5281 ctccccttcg gtcggcacat ggtaccggac gctggttgcc gccggtacgc gctgcattgc
     5341 tccgggccgg acgcaacgtc gagcgaagcc gtcttgcggg tgatcttgcc ggctcgaggc
     5401 tgagcgcgtc agtggccgcc gatgacgttc ttcatcaccg cgatcgtcag gccgccgacc
     5461 aacacgacgg caccgacgat gacggcacgg cgttgcgagc cgccgttcgc gcgcgccgcc
     5521 gcgtagcccg agagcgccag caccacggcg agcatgacgc gcacgacgtc gagctcggcc
     5581 gactcgggca ccaagaaaac ggggatcgtg cagatcgtcg cgacgctcag cgcgcccgcg
     5641 agttgctcgc gcgcggtgcg ccactcgtgc ggcgcggcct cgccgtggac gaggtgcacc
     5701 gccaacacga acgcgaagaa gtgcgcgatc gcgagcccga acgactcgcc ccacaccacc
     5761 gcgaggatgc cgatgtcgtg catgtcggcg tcagtgagca ggctggtcgt cgcgagcaac
     5821 acgatggtga cgtacaacgc catcgtgctc gactcgacgc cggtaccgat ccccagcgtc
     5881 cacttcggat acgcccgttc ccgctgctgc accacgagcg agaacggtaa ccggccgcca
     5941 cctcgtgtcc gattcaggcg ggaaacgccc atgtcacgcg cgtgtcacgc cttctggttg
     6001 tacgcccgaa cccggaggtc gccgccatgc cgagtgcccg ccgttccttt gccgtcattg
     6061 ccatcgtcgc catcgcgctg accatgctcg tcacgggcac ggccgacgcc ggcatcgtca
     6121 tcccgcgcga cgacgccacc gtcatcgacg gaccgaccgt cgcgcaggaa gggccctcgg
     6181 ttgtgatcga gtggtccatg cccaaacgct tcggtctccc gcaaacgaac gcgtatgtgc
     6241 ggaacagcgc gggctggaag gtcaccgtca cgacgtgtgg ttcgtacacg aacgcgagcg
     6301 gcgtcacgat caccggctcg tcgtggatct tgtacgacaa gggagtccaa ctcgcgtcgt
     6361 cgacgacctg caagaagaca atcacgctgc cgcatctggg gttctacgac gtcaagggca
     6421 cgatacggac gtcgaacggc ggcacctaca cggcacaacg gcgcatcgag ctgaaggacc
     6481 acctcatcgt cgtcgcgggc gactcgatgg cctcgggcga aggcaacccc gatgtgaagg
     6541 gcgacgccga catctcgtgg tgcggcagct cgaagctcga agagcagctc atgccctggt
     6601 cgtgtgtcgt gcagatgatc gacctcatca cgaacaaccc gtcggtgctc gtcg
//
LOCUS       DASUGA010000338         8175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2070072, whole genome shotgun sequence.
ACCESSION   DASUGA010000338 DASUGA010000000
VERSION     DASUGA010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8175
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2070072"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VFX21_06725"
     CDS             complement(<1..220)
                     /locus_tag="VFX21_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX5095684.1"
                     /translation="MTGRLLAQIVSLTPSNYEAFARCPRLFFNESLLGIPASDAGISS
                     DQGMLVHALIYKIHKSGSCADNAHVTDVL"
     gene            complement(217..906)
                     /locus_tag="VFX21_06730"
     CDS             complement(217..906)
                     /locus_tag="VFX21_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095685.1"
                     /translation="MDDELPRVTPAMLRRADDMCRRRLAREHAGGKKYANKGADARFA
                     VSNRLVEDARLAHAEAGPPRAEAFVDPGELEPEQIHLYRAAVRGYLALFGEQSGRAFD
                     LGWRTTLAHLGVDLTGNPGLAFAPDGGAPELRMLKVGGRAPDDVDVNVALLRTEEWAP
                     DALRIVVADVIWMEHTELSIDLPEARAAAHEWIDERVAVVQRNAAHGQPRAGADCNGC
                     AFIAGCKAHPQ"
     gene            969..1418
                     /gene="rpiB"
                     /locus_tag="VFX21_06735"
     CDS             969..1418
                     /gene="rpiB"
                     /locus_tag="VFX21_06735"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX5095686.1"
                     /translation="MRIAIGADHAGFELKGHFSQLLKDWGHDVEDLGTYTPEMIDYPP
                     ICASVGRKVVSGGADAGIVIGGSGQGEQIAANKVRGVRAALCNDLFTARLARAHNNAN
                     VLSIGARAVGVTLAEEILKLFLETPFDGGRHATRVAEITAIEEEEAR"
     gene            1415..2659
                     /gene="glyA"
                     /locus_tag="VFX21_06740"
     CDS             1415..2659
                     /gene="glyA"
                     /locus_tag="VFX21_06740"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX5095687.1"
                     /translation="MTTPLERTDPEIADIIRREVERQNTTVQLIASENFASRAVIEAQ
                     GSVLTNKYSEGYPSKRYYGGNYVVDEAEELARRRACDLFGADHANVQPHSGANANMAA
                     FLALLEPGDKVMGMRLDQGGHLTHGSPVNFSGRLYDFVAYGVDDDSEQLDYDAIRALA
                     VQERPKMIIAGATAYPRVIDFPAFRSIADEVGALLMVDAAHIAGLVAGGAHPSPLPLA
                     DIVTFTTHKTLRGPRAGAILCKQEYAAAIDKAVFPGLQGGPLMHVIAAKAVAFREAMA
                     PEFKEYAADIVVNAQALADALGEEGFRIVSGGTDNHLMLVDLRPFGVTGKVAQEALDR
                     AGITCNKNAIPNDPEKPFTTSGLRLGTAACTTVGMGTEEMRQIAAMMSRVLRKPDDKE
                     VATAVREEANDLCARFMPYPEG"
     gene            2663..3853
                     /locus_tag="VFX21_06745"
     CDS             2663..3853
                     /locus_tag="VFX21_06745"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX5095688.1"
                     /translation="MGGYAVVLGAAAVTTLLLTPLVRWLAIRFGAVVMPSQAKHTHEG
                     ALPTLGGAAMFCGFLVAMAVASQIPQFHEMFADNSEPFGIMLAAGVIFVVGALDDLIE
                     VSPPAKMAGQVLAAGMLVFFGVTMFYFRMPFDLFGTDTVVLSQDWAPLVTTLWVVLMT
                     NAINLIDGIDALAAGIVAIAGTALFLFADQLFDAGLLEGSNVAPLIAIIAVGVCLGFL
                     PHNWHPSRIMMGDSGALLLGLLLAVPTITIGGRTDFAFSGSTYFFFAPLLIPIVILGV
                     PIIDTVFSFIRRILSRQSWSLGDSGHLHHRLMRLGHGPRRTVIILWAWTAILSGVALL
                     PVYTDEGNAVVPFVAAALALGLYVFFHPGVRSAREQAARDQHPTGTGSEDVVDLDARR
                     RQRA"
     gene            4070..4351
                     /locus_tag="VFX21_06750"
     CDS             4070..4351
                     /locus_tag="VFX21_06750"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX5095689.1"
                     /translation="MQFGFRTKAAPAPEKHLMQGLDGGVTRGIEMVVTPVLFALLGFW
                     LDGVFGTRPILTVVFFTLALAGAVASVYYRYRAEMDEHEKGKPWTKSPR"
     gene            4330..4761
                     /locus_tag="VFX21_06755"
     CDS             4330..4761
                     /locus_tag="VFX21_06755"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEX5095690.1"
                     /translation="MDEIPAVERELAFDIAKRGLLVSPVVILACGLLRGPDGAWSAAI
                     GLGIVIVNFLVAALIVDRAAKVSTAAVGAASLAGYVLRLVVIVVALLLLRNQPWIDLP
                     VLGFTLVGAQLGLLFWEARHVSLTLAAPGLRPPRPTPSGDQ"
     gene            4758..5516
                     /gene="atpB"
                     /locus_tag="VFX21_06760"
     CDS             4758..5516
                     /gene="atpB"
                     /locus_tag="VFX21_06760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX5095691.1"
                     /translation="MILGVDVPPISHVTIWGSGPLGINKTAVIYVFATLATILLFALG
                     TGKKKPLVPSGIFQNTAESGVGFVRSQIIMSTMGSDGLGYLPYLTALFFFIFFSNITE
                     ILPGVQFPANARLAMPLVFALATWFIYNIVGIARQGPLTYLKNSTIPPGVPKAILPLV
                     MIIEFVSNFIVRPFSLMVRLFANMLAGHLILVTFAALCAALWAAKVTAIIVVPTFGLL
                     VAMTGFEILVSFLQAFIFAILTAVYIGSSMHPDH"
     gene            5577..5825
                     /gene="atpE"
                     /locus_tag="VFX21_06765"
     CDS             5577..5825
                     /gene="atpE"
                     /locus_tag="VFX21_06765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016935169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEX5095692.1"
                     /translation="MFLAQQAADPTKGLTAIGRGIVYGGAAIGPGIGIGLVVGNAITA
                     MARQPESAGTVRTTMFLGIAFTEALALFGFVLAFIISG"
     gene            5842..6507
                     /gene="atpF"
                     /locus_tag="VFX21_06770"
     CDS             5842..6507
                     /gene="atpF"
                     /locus_tag="VFX21_06770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX5095693.1"
                     /translation="MRTRIRKTMAMAAIAVGVIGFAAPAFAQEEEPDFANHAAEECFE
                     KLENGATVDDCQKAPSPLRPEWNEIIWGSAAFLVLLLAAWKWGVPAVKNMEAAREERI
                     RNDLERAETARTEAESEKEQYLAQIADARNEAGRIIEEARQAAEQVRRDTVARAEAEA
                     AEVRARAQEDARLAADRAMSELRGRVGELSIQLAEKIVERNLDRNTQMDLVNSFIDQV
                     GTR"
     gene            6510..7043
                     /gene="atpH"
                     /locus_tag="VFX21_06775"
     CDS             6510..7043
                     /gene="atpH"
                     /locus_tag="VFX21_06775"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEX5095694.1"
                     /translation="MADADARIDVYADALLRIAQQEGYLYEVEDELFRFARVVEGNDD
                     LRIAISDPALPPERRAAIVEELLANKAMQTTIAIVSFIIGIGRGRDLPAIVEKFVELS
                     ALRREKAVAEVRSAVPLSDEQVQRLAAALSTSTGKRVEVKVIVDEKVMGGLVARIGET
                     VIDGTVRTKLEKFKETM"
     gene            7052..>8175
                     /gene="atpA"
                     /locus_tag="VFX21_06780"
     CDS             7052..>8175
                     /gene="atpA"
                     /locus_tag="VFX21_06780"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX5095695.1"
                     /translation="MTINPDDITAVLKKHVEGFAPSISKEQVGRVLEVGDGIARVAGL
                     PNAAVNELLEFEGGAQGLALNLEEGTIGAVILGVADIVEEGMQVTATGRILSVPVGDV
                     MLGRVVNSLGVAIDGKGPIDQKNLRRLEVQAPGITGRKPVHEPLQTGIKAIDAMTPIG
                     RGQRELIIGDRKTGKTTIAVDTILNQKGQGVKCIYVAIGQKGSTVAQTVETLREHGAL
                     EYTVVVSAPASDEAVFKYLAPYAGCAIGQHWMENGEHALVVYDDLSKQAEAYRQLSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDRGAGSLTALPVIETKAGDVSAYIPTNV
                     ISITDGQVYVQDNLFKSGVRPAVDVGNSVSRVGGAAQLKS"
BASE COUNT         1308 a         2769 c         2738 g         1360 t
ORIGIN      
        1 cgagcacgtc ggtgacatgt gcgttgtcgg cgcacgaccc gctcttgtgg atcttgtaga
       61 tgagcgcgtg cacaagcatg ccctggtcgc tggagatgcc cgcgtcgctg gccgggatgc
      121 cgagcagcga ctcgttgaag aacaggcgcg ggcaccgcgc aaaggcctcg tagttgctcg
      181 gcgtcaacga cacgatctgg gcgaggaggc ggccggtcat tgcggatgcg ccttgcagcc
      241 ggcgatgaac gcacaaccgt tgcagtcggc acccgcgcgc ggttggccgt gcgccgcatt
      301 gcgctgcacg accgcgacgc gctcgtcgat ccactcgtgc gcggccgcgc gcgcctccgg
      361 cagatcgatc gacagctcgg tgtgctccat ccagatgacg tcggcgacga cgatccgcaa
      421 cgcgtcgggg gcccactctt cggtgcgcaa caacgcgacg ttgacgtcga cgtcgtcggg
      481 tgcgcgcccg ccgaccttga gcatgcgcaa ctccggtgcg ccaccgtcgg gcgcgaacgc
      541 gagcccgggg ttgccggtca agtcgacgcc gagatgggcc aacgtcgtgc gccacccgag
      601 atcgaacgcg cgccctgact gctcgccgaa caacgcgaga tacccgcgca ccgccgcgcg
      661 atagagatga atctgttcgg gttccagctc gccgggatcg acgaacgcct ccgcgcgcgg
      721 cgggccggct tccgcgtgcg caaggcgagc gtcttcgacg agacggtttg acaccgcgaa
      781 gcgagcgtcg gcacccttgt tcgcgtactt cttgccgccc gcgtgctcgc gggccagccg
      841 ccggcggcac atgtcgtcgg cccggcgcag catggccggt gtgacccgcg gcagctcgtc
      901 gtccacggat cggacgctaa cgcccacatg agacaacccc gggagatccc gtctcataca
      961 ctcgccgggt gcggattgcg atcggtgccg accacgccgg cttcgaactg aagggccact
     1021 tcagccagct cctgaaggac tggggccacg acgtcgagga tctcggcacg tacacgcccg
     1081 agatgatcga ctacccgccg atctgcgcga gcgtcggtcg caaggtcgtc tccggtggcg
     1141 ccgacgcggg catcgtgatc ggcggcagcg gccagggcga acagatcgcc gccaacaagg
     1201 tgcgcggggt gcgcgccgcg ctgtgcaacg acttgttcac ggcccggttg gcccgcgccc
     1261 acaacaacgc gaacgtactc tcgatcggcg cgcgcgccgt gggtgtcacc ctcgccgaag
     1321 agatcttgaa gctgttcctg gagacgccgt tcgacggtgg gcgacacgcc acccgcgtcg
     1381 ccgagatcac cgccatcgaa gaggaggaag cgcgatgacg acgccgctcg aacgcaccga
     1441 tcccgagatc gccgacatca tccgccgcga ggtcgagcgc cagaacacca ccgtgcagct
     1501 catcgcgtcc gagaacttcg cgtcgcgtgc ggtgatcgaa gcgcagggtt cggtcctcac
     1561 gaacaagtac tccgagggtt atccgtccaa gcggtactac ggcggcaact acgtcgtcga
     1621 cgaagccgag gagttggcgc ggcgccgggc gtgcgacctg ttcggcgccg accacgcgaa
     1681 cgtgcagccg cactcgggtg cgaacgcgaa catggcggcc ttcctcgcgc tgctcgagcc
     1741 gggcgacaag gtcatgggca tgcggctcga ccagggcggc cacctcacgc acggctcgcc
     1801 cgtcaacttc agcggccgcc tctacgactt cgtcgcctac ggcgtcgacg acgacagcga
     1861 acagctcgac tacgacgcga tccgggcgct ggccgtgcag gaacggccga agatgatcat
     1921 cgcgggcgca actgcgtacc cgcgcgtcat cgactttccc gcgttccggt cgatcgccga
     1981 cgaggtcggc gcgctgttga tggtcgatgc cgcgcacatc gccggtctgg tggccggtgg
     2041 cgcgcacccg tcgccgctgc cgctcgccga catcgtgacg ttcacgaccc acaagacgct
     2101 gcgcggcccg cgtgcgggcg ccatcctctg caagcaggag tacgccgccg cgatcgacaa
     2161 ggcggtgttc ccgggcctgc agggcggccc gctcatgcac gtcatcgcgg ccaaggccgt
     2221 cgcgttccgg gaggcgatgg cccccgagtt caaggagtac gcggccgaca tcgtcgtgaa
     2281 cgcgcaggcg ctggccgacg cgctcggcga agagggcttc cgcatcgtgt ccggcggtac
     2341 cgacaaccac ttgatgctcg tcgacctccg gccgttcggg gtgaccggca aggtcgcgca
     2401 ggaagcgctg gatcgcgccg gcatcacctg caacaagaac gcgatcccga acgaccccga
     2461 gaagcccttc acgacgagcg ggctgcgtct cggcaccgcg gcgtgcacga cggtgggcat
     2521 gggcaccgag gagatgcgcc agatcgcggc gatgatgtcg cgcgtgctgc gcaagcccga
     2581 cgacaaagaa gtcgcgacgg cggtacgcga agaagccaac gacctctgcg cccgcttcat
     2641 gccgtacccc gagggctaac gcatgggggg ttacgcggtc gtcctggggg cggccgcagt
     2701 gacgacgctg ttgctgacgc cgctggtgcg gtggctggcg atccgtttcg gcgccgtcgt
     2761 gatgccgtcg caggcgaagc acacgcacga gggcgcgttg ccgacgctcg gcggcgcggc
     2821 gatgttctgc ggcttcctcg tcgcgatggc ggtcgcgtcg cagatcccgc agttccacga
     2881 gatgttcgcc gacaactccg agccgttcgg gatcatgctc gccgcgggcg tgatcttcgt
     2941 cgtcggcgcg ctcgacgacc tcatcgaagt gtcgccgccc gccaagatgg ccggacaggt
     3001 gctcgcggcc ggcatgctcg tgttcttcgg cgtgacgatg ttctacttcc gcatgccgtt
     3061 cgacttgttc ggcaccgaca cggtcgtgct gtcgcaagac tgggcgccgc tcgtcacgac
     3121 actgtgggtc gtgttgatga cgaacgcgat caacctcatc gacggcatcg acgcgctcgc
     3181 ggccggcatc gtcgcgatcg ccggcactgc gttgttcttg ttcgccgatc agctgttcga
     3241 cgcggggcta ctcgaaggat cgaacgtcgc accgctcatc gcgatcatcg cggtgggtgt
     3301 gtgcctcggc ttcctgccgc acaactggca tccgtcgcgc atcatgatgg gtgactccgg
     3361 cgcgctgttg ctcggcctgc tgctcgcggt accgacgatc acgatcggcg gtcgcaccga
     3421 cttcgcgttc tccggcagca cgtacttctt cttcgcgccg ctgttgatcc cgatcgtcat
     3481 cctcggtgtc ccgatcatcg acaccgtgtt ctcgttcatc cggcggatcc tctcgcgcca
     3541 gagttggtcg ctcggtgact ccgggcacct gcaccaccgc ttgatgaggc tcggccacgg
     3601 tccgcgccgg acggtcatca tcctgtgggc ctggaccgcg atcctctcgg gcgtcgcgct
     3661 gctgcccgtc tacaccgacg agggcaacgc ggtggtgccc ttcgtggccg cggcgctggc
     3721 cctcggcctc tacgtcttct tccaccccgg cgtacggtcc gcgcgcgagc aagcggcccg
     3781 cgatcagcac cccaccggga cgggcagcga ggacgtcgtc gacctcgacg cccggcgccg
     3841 ccagcgggcc tgacgttcgc cggacatcgc ggtggccgac cggggagtga ctggtccgac
     3901 gccggtcgac gggcgccgga ccgggtcggt agggtcgctc cttacctgac ggggaatcgc
     3961 ttcggtttga ctcttcgcgg agactgaccg tagattgcga atacattcac aagccgcctc
     4021 cggagcccgt cgcggggcgg ggtggcgtga acaacggggt tcaccagcaa tgcaattcgg
     4081 gtttcgcacg aaggccgcac cggcgcccga gaagcacctc atgcaagggc tcgacggggg
     4141 agtgacgcgc ggcatcgaga tggtcgtcac gcccgtgctg ttcgcgctgt tgggcttctg
     4201 gctcgacggc gtcttcggca cgcgaccgat cctgaccgtc gtgttcttca cgctggcgct
     4261 cgccggcgcc gtcgcctccg tctactaccg ctaccgcgcc gagatggacg agcacgagaa
     4321 ggggaaaccg tggacgaaat ccccgcggta gagcgcgagc tggccttcga catcgccaag
     4381 cgcgggctgc tcgtcagtcc cgtcgtcatc cttgcgtgtg gcctcttgcg cggccctgac
     4441 ggtgcgtggt ccgccgcgat cgggctcggc atcgtcatcg tcaacttctt ggtcgccgcg
     4501 ctcatcgtcg accgcgccgc caaagtctcg accgctgcag tcggcgccgc gtcgctcgcg
     4561 ggctacgtgc tgcgactcgt cgtgatcgtt gtcgcactat tacttttgcg caaccaacct
     4621 tggatcgacc tccccgtact cgggttcacg ctcgtcggcg cgcaactcgg gttgttgttc
     4681 tgggaggcgc gccacgtgag tttgacgctt gccgcgccgg gccttcgccc gccgcgtccg
     4741 accccatcgg gagaccaatg atcctcggtg tcgatgttcc gccgatcagc cacgtcacca
     4801 tctggggatc ggggccgctc ggaatcaaca agaccgcggt catctacgtc ttcgcgacgt
     4861 tggccacgat cctgttgttc gcgcttggta ccggcaagaa gaagccgctc gtgccgtcgg
     4921 gcatcttcca gaacaccgcg gagtccggtg tcgggttcgt gcgcagccag atcatcatgt
     4981 cgacgatggg cagcgacggc ctcggctacc tgccgtacct gactgcgctg ttcttcttca
     5041 tcttcttcag caacatcacc gagatcctgc ccggcgtgca gttccccgcc aacgcgcgcc
     5101 tcgcgatgcc gctggtcttc gcgctggcga cgtggttcat ctacaacatc gtcggcatcg
     5161 cccggcaggg cccgctgacc tacctgaaga actcgacgat cccgcccggc gtgcccaagg
     5221 cgatcctgcc gctcgtgatg atcatcgagt tcgtgtcgaa cttcatcgtt cgcccgttct
     5281 cgttgatggt ccggctcttc gccaacatgc tcgccggcca cctcatcctg gtgacgttcg
     5341 cggcgctgtg cgcggcgctg tgggcggcga aggtgaccgc gatcatcgtc gtgccgacct
     5401 tcggcctgct cgtcgccatg accggattcg agatcctcgt cagcttcttg caggcgttca
     5461 tcttcgcgat cttgaccgcg gtgtacatcg gctcgtcgat gcaccccgac cactagtcca
     5521 tcgatcaaca cagtccgagc aaacagagaa agagggagac cttcgtggcc ctactgatgt
     5581 tcttggccca gcaggctgcc gaccccacca agggtctgac ggcgatcggg cgcggcatcg
     5641 tctacggcgg cgcggccatc ggccccggca tcggcatcgg cctcgtggtc ggcaacgcca
     5701 tcaccgcgat ggcccgccag cccgagtccg ccggcaccgt ccgcacgacg atgttcctcg
     5761 gtatcgcgtt caccgaggcg ctcgcgctgt tcggcttcgt ccttgcgttc atcatctccg
     5821 gctgacccaa aaggagccac ggtgcgtact cgcatccgta agaccatggc gatggcggcc
     5881 attgccgtag gcgtgatcgg tttcgccgcg cctgcgttcg cgcaggagga agagcccgac
     5941 ttcgcgaacc acgcggccga ggagtgcttc gagaaactcg agaacggcgc gacggtcgac
     6001 gactgccaga aggcaccgag cccgctgcgt cccgagtgga acgagatcat ctggggttcc
     6061 gccgcgttcc tcgtgttgct gctcgccgcc tggaagtggg gcgtccccgc cgtcaagaac
     6121 atggaggcgg ctcgcgagga gcgcatccgc aacgatctcg agcgcgccga aacggcgcgc
     6181 accgaggccg agagcgagaa ggagcaatac ctcgcgcaga tcgcggacgc tcgcaacgag
     6241 gccggtcgca tcatcgagga agcgcgccag gccgcggagc aggtgcgccg cgacacggtt
     6301 gctcgcgcgg aggccgaagc ggccgaggtg cgggcccgtg cccaagaaga cgcgcgcctc
     6361 gccgccgatc gggcgatgtc ggagctgcgt ggtcgggtcg gtgagctgtc gatccagctc
     6421 gcggagaaga tcgtcgagcg caacctcgac cgcaacacgc agatggacct cgtgaacagc
     6481 ttcatcgacc aggtcgggac ccggtagcga tggccgacgc cgacgcccgc atcgacgtct
     6541 acgcggacgc gctgctgcgc atcgcgcagc aggagggcta cctctacgag gtcgaagacg
     6601 agctcttccg cttcgcgcgt gtggtggagg gcaacgacga tcttcgcatc gccatctccg
     6661 acccggcgtt gccgccggag cgccgcgccg cgatcgtcga ggagctgctc gccaacaagg
     6721 cgatgcagac gacgattgcg atcgtgtcgt tcatcatcgg gatcggccgc ggtcgcgacc
     6781 tcccggcgat cgtcgagaag ttcgtggagc tctcggcgtt gcggcgcgag aaggcagtgg
     6841 ccgaggtgcg cagcgcggtg ccgctgtccg acgagcaggt gcagcggttg gccgcggcgc
     6901 tcagcacgtc gaccggcaag cgcgtggaag tgaaggtgat cgtcgacgag aaggtgatgg
     6961 gcggcctcgt cgcccgcatc ggtgagacgg tcatcgacgg cacggtccgt accaagctcg
     7021 agaagttcaa ggagacgatg tagtggctga gttgacgatc aacccagacg acatcactgc
     7081 ggtcctcaag aagcacgtcg agggcttcgc gccgtcgatc tccaaggagc aggtcggccg
     7141 cgttctcgaa gtcggtgacg gcatcgcgcg cgtcgcgggc cttcccaacg cggcggtcaa
     7201 cgaactgctc gagttcgagg gtggcgcgca aggtctcgcg ctgaacctcg aagagggcac
     7261 gatcggtgcg gtcatcctcg gtgtcgcgga catcgtcgaa gagggcatgc aggtcactgc
     7321 gaccgggcgc atcctctcgg tgccggttgg cgacgtcatg ctcggacgtg tcgtcaactc
     7381 gctcggtgtc gcgatcgacg gcaagggccc gatcgaccag aagaacctcc gccgcctcga
     7441 ggtgcaggcg cccggcatca ccggtcgcaa gccggtgcac gagccgctgc agaccggcat
     7501 caaggccatc gacgcgatga cgcccatcgg tcgtggtcag cgggagctga tcatcggcga
     7561 ccgcaagacg ggcaagacga cgatcgcggt cgacacgatc ctgaaccaaa agggtcaggg
     7621 cgtgaagtgc atctacgtcg ccatcggtca gaagggctcg acggtcgcgc agaccgtgga
     7681 gacgctgcgt gagcacggtg cactcgagta cacggtcgtg gtgagtgcgc ccgcctccga
     7741 cgaagcggtc ttcaagtacc tcgcccccta cgcgggttgc gcgatcggtc agcactggat
     7801 ggagaacggc gagcacgcgc tcgtcgtgta cgacgacctg tcgaagcagg ccgaggcgta
     7861 ccgccagctg tcgctgctgc tgcgccggcc gccgggccgc gaggcgtacc ccggtgacgt
     7921 cttctatctc cactcgcgcc tgctggagcg cgccgcgaag ttgagcgacg accgcggtgc
     7981 cggttcgctg accgcgttgc cggtcatcga gaccaaggcg ggtgacgtct cggcgtacat
     8041 cccgaccaac gtgatctcga tcaccgacgg tcaggtgtac gtgcaggaca acctgttcaa
     8101 gtccggcgtg cgcccggcgg tcgacgtcgg caactcggtg tcgcgcgtgg gcggcgccgc
     8161 acagctcaag tcgat
//
LOCUS       DASUGA010000339         3190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1670137, whole genome shotgun sequence.
ACCESSION   DASUGA010000339 DASUGA010000000
VERSION     DASUGA010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1670137"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VFX21_06785"
     CDS             <1..203
                     /locus_tag="VFX21_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020109620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX5095696.1"
                     /translation="LGGEIPSPMAPPSGCRFRTRCPKAQELCAAEEPVIRELRDGQYV
                     ACHFPLAPGEVLPGTADDALVV"
     gene            complement(275..1435)
                     /locus_tag="VFX21_06790"
     CDS             complement(275..1435)
                     /locus_tag="VFX21_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5095697.1"
                     /translation="MDFTYPPEAEAFRAEVRAWLDANLPADLAHRRTTLDTDAETLAR
                     LRAWNRALADARYAAIAWPEEWGGRGASVMEQVVYAEEMHRAGAPGTLNPIGLSNIAP
                     AIIEHGTDEQKRQLLPRMLRGDDIWCQGFSEPNAGSDLASLRTRAVADGDDWVVNGQK
                     TWTTLGHLANWCELLVRTDPDVPKHKGITCLLVDMTLPGIEVRPLITITGERDFNEIF
                     FTDVRVPRSATLGPVNEGWRVAMTTLAYERGTVAKLHLGTRAKVQRLIELARTTPLGD
                     GRMAADDPVLRTKLASVYLDAELLKLISDRALSAELHGRAMGPEGSVAKLLWSETEQR
                     VADVASDVLGPIALSGDWAHDRVYSRSFTIAGGTTQVNKNIVAQRMLGLPRA"
     gene            complement(1435..2268)
                     /locus_tag="VFX21_06795"
     CDS             complement(1435..2268)
                     /locus_tag="VFX21_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5095698.1"
                     /translation="MKLIEPRMPELSLTQEHQMCPGCGEPLAARQFLDAISDLGVARR
                     SIAVVGIGCYTSFSGTIDVDLVQALHGRAPSVATGVKRMQPDALVFTLQGDGDMVNEG
                     LQEVVHTAARGERLTCILLNNGVFGETGGHMTATSVLGQRTKNTLDGRDAEYHGYPIL
                     IGDLIAQLEGAAYVARGSVHNAGAVARTKKMFRRAFESQLRGDGFSFVEVLTMCPTGW
                     FIPTAEGPGYMTETLGEVHIMGELKVDGRVRTTDELHAENVAKQTEMDARLAAGGLDH
                     D"
     gene            complement(2265..>3190)
                     /locus_tag="VFX21_06800"
     CDS             complement(2265..>3190)
                     /locus_tag="VFX21_06800"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095699.1"
                     /translation="GTRAATGSTGQGLSLMQESFSEITLAELPLVIFNMARGQQDYYQ
                     ATRGGGHGDYRHIVLAPQDVREGVEHVQLAFHLADKWRNPVLVYGDYLLAHTQEALTI
                     ETIAFDPLPPKTWAVDGTASGSGASKSVTPLGVAKYGQPAFGQEGKAQYIATKTPLME
                     REVRVESGFLDGAETVIVAFGTPAKFVKYAIKQLRAQGHRIGYVRPITLWPFPYDAVR
                     DACTDTVQRVGSFELCAGQMVDDVRIGVAGRAPVSFIGGVSTDHSGFGVGRLLDVEAI
                     TERILALHHDRPMPLIPGYEQFSYELQEHQV"
BASE COUNT          457 a         1073 c         1121 g          539 t
ORIGIN      
        1 cgctcggcgg tgagatcccg tcgccgatgg cgccgccgag cggctgccgc ttccgtactc
       61 gctgcccgaa ggcccaggag ctgtgcgcgg ccgaggagcc ggtcatccgt gagctgcgcg
      121 acggccagta cgtcgcctgc cacttcccgc tcgcccccgg agaggtactg cccggcaccg
      181 ccgacgacgc cctggtcgtc tagctgcgcg gtaagcccag aagtcaccca ataccatgcc
      241 ccggccacca cccctgatgg ccggtttgcg ctcgctacgc tcgcggcaaa ccgagcatcc
      301 tctgggccac gatgttcttg ttcacctggg tggtgccgcc cgcgatcgtg aacgagcggc
      361 tgtagacgcg gtcgtgggcc cagtcgccgg agagcgcgat gggcccgagc acgtcgctcg
      421 cgacatcggc cacgcgctgt tcggtctcgc tccacagcag cttggcgacg ctgccttcgg
      481 gtcccatcgc gcggccgtgc aactccgcgg acagcgcgcg atcggagatg agcttgagca
      541 gctccgcatc gagatagacg ctcgcgagct tggttcgcag cacgggatcg tcggcggcca
      601 tgcggccgtc accgagcggt gtcgtgcgcg cgagctcgat gaggcgttgg accttggcgc
      661 gcgtcccgag gtgcagcttg gcgaccgtcc cgcgttcgta cgcgagcgtc gtcatcgcga
      721 cgcgccagcc ctcgttcacc ggcccgagcg tcgcgctccg agggacgcgc acgtcggtga
      781 agaagatctc gttgaagtcg cgttcaccgg tgatcgtgat cagcgggcgc acttcgatgc
      841 cgggcaacgt catgtcgacg aggaggcagg tgatgccctt gtgcttcggg acgtcggggt
      901 cggtgcgcac cagcagctcg caccagttgg cgagatgccc gagcgtggtc cacgtcttct
      961 gcccgttcac gacccagtcg tcaccgtcgg cgacggcgcg cgtgcggagg ctcgcgaggt
     1021 cggagccggc gttgggttcg gagaagccct ggcaccagat gtcgtcgccg cgcagcatgc
     1081 gggggagcag ctgtcgcttc tgctcgtcgg tgccgtgctc gatgatcgcg ggtgcgatgt
     1141 tcgagagccc gatcgggttc aacgtgcccg gcgcgccggc gcggtgcatc tcctcggcgt
     1201 acacgacctg ttccatgacg ctcgcgccgc gcccgcccca ctcttcgggc cacgcgatcg
     1261 cggcgtagcg cgcgtcggcg agggcgcggt tccacgcgcg gagtcgcgca agggtctcgg
     1321 cgtccgtgtc gagcgtcgtc cgccggtgcg cgaggtcagc gggcagattg gcgtcgagcc
     1381 acgcccggac ctcggcgcgg aacgcctcgg cttcgggcgg ataggtgaag tccatcagtc
     1441 gtggtccagt ccgccggcgg cgaggcgcgc gtccatctcg gtttgcttgg cgacgttctc
     1501 ggcgtggagc tcgtcggtgg tgcggacgcg gccgtcgacc ttgagctcgc ccatgatgtg
     1561 cacttcgccg agcgtctcgg tcatgtagcc gggcccttcc gcggtcggga tgaaccaacc
     1621 agtggggcac atggtgagca cctcgacgaa cgagaagccg tcgccgcgca actgcgactc
     1681 gaacgcacgg cggaacatct tcttcgtgcg ggctaccgcg cccgcgttgt gcaccgatcc
     1741 gcgcgccacg taggcggcgc cttcgagctg cgcgatcagg tcaccgatca ggatcgggta
     1801 gccgtggtac tcggcgtcgc ggccgtcgag cgtgttcttc gtgcgctggc cgagcacgct
     1861 cgtcgcggtc atgtgcccgc cggtctcgcc gaagacgccg ttgttcaaca ggatgcacgt
     1921 caaccgctca ccgcgtgcgg cggtgtggac gacttcctgg agtccttcgt tgaccatgtc
     1981 gccgtcgcct tgcagcgtga acacgagcgc gtcgggttgc atccgcttca ccccggtcgc
     2041 gaccgacggc gcgcgcccgt gcagcgcctg cacgaggtcg acgtcgatcg tgcccgagaa
     2101 cgacgtgtag cagccgatcc cgacgaccgc gatggagcgt cgtgcgacac cgaggtcgga
     2161 gatcgcgtcg aggaactgcc gcgccgcgag cggttcgccg cagccagggc acatctggtg
     2221 ctcctgggtg agcgacagct cgggcatccg cggttcgatc aacttcatac ctggtgctcc
     2281 tggagctcgt aggagaactg ttcgtagccg gggatgaggg gcatcggtcg gtcgtggtgc
     2341 aacgcgagga tgcgttcggt gatcgcctcg acgtcgagca agcgcccgac accgaacccg
     2401 gagtggtcgg tcgacacgcc gccgatgaac gacaccggcg cgcggccggc gacaccgatg
     2461 cggacgtcgt cgaccatctg ccccgcgcac agctcgaacg aaccgacccg ctgcaccgtg
     2521 tccgtgcacg cgtcgcgtac ggcgtcgtag gggaacggcc acaaggtgat gggccggacg
     2581 tagccgatgc gatgaccttg cgcgcgcagt tgcttgatcg cgtacttcac gaacttggcc
     2641 ggcgtgccga acgcgacgat cacggtctcg gcgccgtcga ggaatcccga ctcgacgcgc
     2701 acctcacgtt ccatgagtgg cgtcttcgtc gcgatgtact gcgctttgcc ctcctgcccg
     2761 aaggccggtt ggccgtactt cgcgacgccg agcggtgtca ctgacttcga tgcgccggag
     2821 cccgacgcag tgccgtcgac tgcccacgtc ttcggcggca gcgggtcgaa ggcgatcgtc
     2881 tcgatcgtga gggcttcctg ggtgtgcgcg agcaagtagt cgccgtagac gagcacgggg
     2941 ttgcgccact tgtcggcgag atggaacgcg agctgtacgt gctcgacgcc ctcgcgcacg
     3001 tcttgcggtg cgaggacgat gtggcggtag tcgccgtggc caccgccgcg cgtcgcctgg
     3061 tagtagtcct gctgaccgcg ggccatgttg aagatgacga gcggcagctc cgcgagcgtg
     3121 atctccgaga acgactcctg catgagcgac aagccctgcc cggtcgagcc cgtcgcggcg
     3181 cgggtaccgg
//
LOCUS       DASUGA010000340         2052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1946300, whole genome shotgun sequence.
ACCESSION   DASUGA010000340 DASUGA010000000
VERSION     DASUGA010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1946300"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /gene="meaB"
                     /locus_tag="VFX21_06805"
     CDS             complement(<1..910)
                     /gene="meaB"
                     /locus_tag="VFX21_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX5095700.1"
                     /translation="MSRTDTGELVRLACAGDRRAVARLISLVETGGDPAREALAALYP
                     STGRAYSVGITGAPGAGKSTLTDRLVARVRADGERVAVLAVDPTSPFSGGAILGDRVR
                     MQDHATDPGVFIRSMATRGHLGGLALATPQAIRVLDAAGAPWILVETVGVGQVEVEVA
                     AATDTTVVVVNPGWGDSVQANKAGLLEIADVFVVNKADRPGADDAERDLLQMLELGAH
                     RAWMPPIVRTVATDGEGIDALWDAIGQHRAHLEETGELVRRRRDRLRDEVRSIVLARL
                     AAEVDARVASDGGLDALVARVDAREID"
     gene            complement(907..1164)
                     /locus_tag="VFX21_06810"
     CDS             complement(907..1164)
                     /locus_tag="VFX21_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX5095701.1"
                     /translation="MSTETANRDDMEAGVLEAIEAIRPALQSDGGDIVFKRIDDDGVV
                     FVDLIGACGTCPVSTMTLKAGVERIIMDRVPGITAVEEDGA"
     gene            complement(1213..>2052)
                     /locus_tag="VFX21_06815"
     CDS             complement(1213..>2052)
                     /locus_tag="VFX21_06815"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX5095702.1"
                     /translation="DVVHGFIDARVNALCGARIVLDYPSVGATETLLTAAVLAKGETV
                     IDNAAREPEITDLVAFLTKMGARIEGAGTNTITVEGVDELGSADHAIVGDRIEGGTFL
                     FAALIAGGDVTVEGIELEHLGVVAAKLADLGANVDRAPDGVRVIVEGRPRATDVQTLP
                     FPGFATDFMPFAVTALAVSDGTGIVTENVFDNRLSFADELNRMGAHVHTEGRHAVVRG
                     VERLSGAPVRALDVRAGAALVLAGLAAEGETVVLESRHIDRGYPDLAGKLRALGADVD
                     RES"
BASE COUNT          303 a          736 c          722 g          291 t
ORIGIN      
        1 gatcgatctc gcgcgcatcg acgcgcgcga ccagcgcgtc caacccgccg tcggacgcaa
       61 cgcgcgcgtc gacctcggct gcgagacgcg ccagcacgat gctgcgcact tcgtcgcgca
      121 atcggtcgcg acgccgacgc acgagctcgc cggtctcctc gaggtgcgca cggtgttggc
      181 cgatggcgtc ccacagcgcg tcgatgccct ccccatcggt cgcaacggtg cgcacgatcg
      241 gcggcatcca cgcgcggtgc gcgccgagct cgagcatctg caacaggtca cgctcggcgt
      301 cgtcggcgcc gggccggtcg gccttgttca ccacgaagac gtcggcgatc tcgaggagcc
      361 ccgccttgtt ggcttgcacg gagtcacccc agcccgggtt cacgacgacg acggtggtat
      421 cggtggcggc cgcaacctcc acttcgacct gccccacccc gacggtctcg acgaggatcc
      481 acggcgcgcc ggcggcgtcg agcacgcgga tggcctgcgg ggtcgcgagg gcgaggccac
      541 cgaggtgacc gcgcgtggcc atcgagcgga tgaagacacc ggggtcggtg gcgtgatcct
      601 gcatgcgcac gcggtccccg aggatcgcgc cgcccgagaa cgggctcgtg ggatcgacgg
      661 cgaggaccgc gacccgctcg ccgtcggcgc ggacacgggc gacgagccga tcggtgaggg
      721 tggacttgcc ggctcccggc gcgccggtga tgccgaccga gtacgcgcgg ccggtgctcg
      781 ggtagagcgc ggcaagggcc tcacgagccg ggtcgccgcc ggtctcgacc agcgagatca
      841 ggcgcgcgac ggcacgccga tcacccgcac acgcgaggcg gacgagctcg ccggtgtcgg
      901 tcctgctcag gctccgtctt cctcgaccgc ggtgatgccg gggacgcggt ccatgatgat
      961 ccgctccacc cccgccttca gggtcatcgt cgagaccggg cacgtgccgc aggcaccgat
     1021 gaggtcgacg aacaccacac catcgtcgtc gatgcgcttg aagacgatgt cgccaccgtc
     1081 ggactggagc gcaggacgaa tcgcctcgat cgcctcgagg accccggctt ccatgtcgtc
     1141 acggttcgca gtctcagtcg acatcgcagt gctcccagtc atcgtctgac tagggaaaca
     1201 gggtaccggc ggtcaggatt cccggtcgac gtcggcgccg agggcccgca gcttgccggc
     1261 gaggtccggg tagccccggt cgatgtggcg gctctccagc acgacggtct cgccctcggc
     1321 cgccagcccg gcgagcacga gcgcggcccc ggcccgcacg tcgagcgcgc gtaccggcgc
     1381 accgctgagc cgctcgacgc cccgcaccac cgcgtgccgt ccttcggtgt gcacgtgcgc
     1441 gcccatgcga ttcagctcgt cggcgaacga cagccggttg tcgaagacgt tctcggtgac
     1501 gatgcccgtg ccgtcactca ccgccagcgc ggtgactgcg aacggcatga agtccgtggc
     1561 gaatcccggg aacgggagcg tctggacgtc ggtggcgcgc ggtcgaccct cgacgatcac
     1621 ccgcacgccg tcgggcgcgc ggtcgacgtt cgcaccgagg tcggcgagct tggcggccac
     1681 gacgccgagg tgctcgagct cgatgccctc gacggtgacg tcgccgccgg cgatcagcgc
     1741 ggcaaacaag aaggtgccgc cctcgatgcg gtcgccgacg atcgcgtgat cggccgaacc
     1801 gagctcgtcg acgccctcga cggtgatggt gttcgtgccg gcgccttcga tgcgcgcgcc
     1861 catcttcgtg aggaaggcga cgaggtcggt gatctcgggc tcacgcgccg cgttgtcgat
     1921 caccgtctca cccttggcga ggactgcggc cgtgagcaag gtctcggtcg cgcccacact
     1981 cgggtagtcg aggacgatgc gcgcgccgca caaggcgttg acgcgcgcgt cgatgaagcc
     2041 gtgcaccacg tc
//
LOCUS       DASUGA010000341         3985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1120394, whole genome shotgun sequence.
ACCESSION   DASUGA010000341 DASUGA010000000
VERSION     DASUGA010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120394"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VFX21_06820"
     CDS             <1..422
                     /locus_tag="VFX21_06820"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5095703.1"
                     /translation="VLGFVVGFRLDAGVAEVALAIALLVAAAYSFMWVFISIGLRSKS
                     AQAANGTATLLIVPLSFISAAYVPADSLPGWLQPVANNQPFTVYSNAMRSLLLGGADA
                     VGLSHSTTYWVGLSLVWCAGILLVFSAIAVARFARRR"
     gene            443..1045
                     /locus_tag="VFX21_06825"
     CDS             443..1045
                     /locus_tag="VFX21_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5095704.1"
                     /translation="MTDSNLDMTMMIAFHDALRRDLQQVAAMDARSEGWDLFERMLHL
                     HHTAEDDLLWPVARDQVAGRSEELALLEEMAAEHAAIGPVLDEFDRELNEGKIAPAVR
                     ADLDERLRQHLQHEEVAALPLIDRTLSPDQWMTFGMAAMERFRPDMPRFLPWLLDGAD
                     ADVTANILKFIPPPVQQSYRDDWLPAYDARDLWATKSSVT"
     gene            complement(1042..2469)
                     /locus_tag="VFX21_06830"
     CDS             complement(1042..2469)
                     /locus_tag="VFX21_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX5095705.1"
                     /translation="MVSGKMRAVLADDIAHWSTTRQAAAIRAGEFSARDLLWLYASRI
                     ERLNPAVNAIVTLDLDRAARDAAAVDERLARGDGVGVGPLAGVPMTIKDAIAVGGMRS
                     TGGAVELRDHVPVADAPAVAALRAAGAVIIGKTNVPRWCNAETETHNEIFGTTNNPWD
                     LQRSVGGSSGGSAAAVAAGLSSSDLGTDIGGSVRIPSHYCGTFALKPSFGVVPQLGYL
                     SHLGAGRVGADMNTFGPITRSADDLALLLDVLAGPAPEDALAWQVALPPPRRSHLAEY
                     RIGTWFDEPDLPVAASYRSVLERAADQLRAAGAHVDVSHPNVGFREQLDLWMTLAGAA
                     ASPSLPKDIREAASGLHMHWLRNHERREELRDRWRMWFDDYDVLLSPAVLTGAPHHDL
                     DGDPFARTIDVDGVPRNIMMDIPQWTGLVNVIGAPAVVAPIGCTDEGLPVGLQIVTSY
                     LRDREAIDVARQVEAVVGGFQAPRL"
     gene            2544..2936
                     /locus_tag="VFX21_06835"
     CDS             2544..2936
                     /locus_tag="VFX21_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5095706.1"
                     /translation="MTDTTTAEIAATPLVDTYFEMWRATDDATRRALLEQVFAPDGRH
                     VDALADANGYDELASMIANVHGHYPGFTIERTSGVDRYGQQLRFGWKLDAADGTPVVA
                     GIDVGELDADGRLTRIAGFWGDLPARDQ"
     gene            complement(2993..3385)
                     /locus_tag="VFX21_06840"
     CDS             complement(2993..3385)
                     /locus_tag="VFX21_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095707.1"
                     /translation="MKLRITALIALALVSVFALAACSTEARAERKGKAAGDQICKAKD
                     ADNADEAQRHIDRANDKLDDLARFTGRDVREDINDLDRNLDQMARGNATSQDVNAIVR
                     SVESARSAASGNAEAAFDGLLEALNGCD"
     gene            complement(3382..>3985)
                     /locus_tag="VFX21_06845"
     CDS             complement(3382..>3985)
                     /locus_tag="VFX21_06845"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEX5095708.1"
                     /translation="VSNRAGMYREVRVTAVEVSYSREPAVGKWWWVLLITGILWIIIG
                     LFVLSADYDSAVLIGYLTGFWLLFAGVAEFMEAGFVAGWKWVHIVLGVFFVLGGIAAL
                     TAPFQTFTILAALIGIFLVIKGSVDFVFGIALRHEVDLWWMTVVAGVIEIIIGIWAVG
                     YPGRSAALLILWIGIGAIIRGIAEIVTAFHVRKDPEAVLA"
BASE COUNT          657 a         1380 c         1328 g          620 t
ORIGIN      
        1 ccgtgctcgg cttcgtcgtc gggttccgcc tcgacgccgg cgtcgccgag gttgccttgg
       61 cgatcgcgtt gctcgtcgcc gcggcgtaca gcttcatgtg ggtgttcatc tcgatcgggt
      121 tgcggtcgaa gagcgcccag gcggccaacg gcacggcgac actgttgatc gtcccgctct
      181 cgttcatctc cgccgcgtac gtgccggcgg actcgttgcc cggttggttg caaccggtcg
      241 cgaacaacca accgttcact gtgtactcca acgcgatgcg gagcttgctc ctgggcggcg
      301 cggacgcggt cggactgagt cacagcacga cgtattgggt gggcctgagc cttgtctggt
      361 gcgcgggaat tcttctcgtg ttcagcgcga tcgccgtcgc gcggttcgcg cgccggcgct
      421 agtccccgat caaggagcac aaatgaccga ctcgaacctc gacatgacga tgatgatcgc
      481 gtttcacgac gcgctgcggc gcgacctgca gcaggtcgcg gcgatggatg cgcgcagcga
      541 gggctgggac ctgttcgaac gcatgttgca cctgcatcac acagctgagg acgacctgtt
      601 gtggcccgtc gcgcgcgatc aggtcgcggg ccgctccgaa gagctcgcgc tgctcgaaga
      661 gatggcggcc gagcacgccg cgatcggacc ggtgctcgac gagttcgacc gcgagctgaa
      721 cgagggcaag atcgccccgg cggtacgcgc cgacctcgac gagcggttgc ggcagcactt
      781 gcagcacgag gaggtcgccg cgctcccgct gatcgatcgc acgctctcac cggatcagtg
      841 gatgacgttc ggcatggccg caatggagcg cttccgtcct gacatgccgc gcttcttgcc
      901 ctggttgctc gacggtgccg acgccgacgt gaccgcgaac atcctgaagt tcattccgcc
      961 gccggtccag cagtcgtacc gcgacgactg gctcccggcc tacgacgcac gcgacctctg
     1021 ggcgacgaag agctccgtga cctagagacg cggcgcctgg aagccgccca caactgcctc
     1081 gacctgacgg gcgacgtcga tcgcctcacg gtcgcgcaga tacgaagtga cgatctgcag
     1141 cccgaccggc aagccctcgt cggtgcagcc gatcggcgcg acaaccgccg gcgcgccgat
     1201 gacgttgacg agcccggtcc actgcgggat gtccatcatg atgttgcgcg ggaccccgtc
     1261 gacgtcgatc gtgcgggcga acggatcgcc gtcgaggtcg tggtgcggcg caccggtcaa
     1321 cacggccggg ctcaacagca cgtcgtagtc gtcgaaccac atgcgccagc ggtcgcgcag
     1381 ctcttcgcgg cgttcgtgat tgcgcagcca gtgcatgtgc agaccactcg cggcttcacg
     1441 aatgtctttc ggcaggctcg gtgacgcggc cgcgcccgcg agcgtcatcc agaggtcgag
     1501 ctgctcgcgg aatccgacgt tcggatgcga cacgtcgacg tgcgcgcccg cggcgcgcag
     1561 ttggtcggcc gcgcgttcga gtaccgaccg atacgacgcc gcgacgggaa gatccggctc
     1621 gtcgaaccac gtaccgatcc ggtactcggc cagatgcgaa cggcgcggcg gcgggagcgc
     1681 aacctgccac gcgagcgcat cttccggcgc cggtcccgcg agcacgtcga ggagcaacgc
     1741 caggtcgtcg gcggaccgtg tgatcgggcc gaacgtgttc atgtcggcgc cgacgcgacc
     1801 cgcgcccagg tggctgaggt acccgagctg cggcaccacg ccgaagctcg gcttcagtgc
     1861 gaacgtgccg cagtagtgcg acgggatgcg caccgaacca ccgatgtcgg tgccgagatc
     1921 agaactgctc aacccggccg ccacggccgc agccgaacca cccgacgagc cgccgacgct
     1981 gcgctgcagg tcccacgggt tgttcgtcgt gccgaagatc tcgttgtggg tctcggtctc
     2041 cgcgttgcac caacgcggca cgttcgtctt gccgatgatc accgcgccgg ccgcgcgcag
     2101 tgccgccacc gcgggcgcgt ccgcgaccgg gacgtggtcg cgcagttcga ccgcgccacc
     2161 ggtgctgcgc atcccgccga ccgcgatggc gtccttgatc gtcatcggca cgccggccag
     2221 cgggccgacg ccgacgccgt cgccgcgcgc cagccgttcg tcgaccgccg ccgcatcgcg
     2281 cgcggctcga tcgaggtcga gcgtgacgat cgcgttgacg gccgggttca gccgctcgat
     2341 gcgagaggcg taaagccaca gcaggtcgcg cgccgagaac tcaccggctc ggatcgccgc
     2401 ggcctgccgc gtcgtcgacc agtgtgcgat gtcgtcggcg agcacagcgc gcatcttgcc
     2461 cgaaaccatg acgtggcacg taatcgcttt gccgcgccgt ttcgtcgatg ctcggggtcc
     2521 cccgacttcc aaggagcacg acgatgaccg acacgactac cgccgagatc gccgccacgc
     2581 cgctcgtcga cacctacttc gagatgtggc gggcgaccga cgacgcaacg cgtcgcgcgc
     2641 tgctcgagca ggtctttgca cccgacggcc gccacgtcga cgcgctggcc gacgcgaacg
     2701 gctacgacga gctcgcgagc atgatcgcca acgtgcacgg gcactacccc ggcttcacga
     2761 tcgaacgcac gagcggcgtc gaccggtacg ggcaacagct gcggttcggt tggaagctgg
     2821 acgccgccga cggcacgccg gtggtcgcgg gcatcgacgt cggcgagctc gacgcggacg
     2881 gccgcctcac gcgcatcgcc ggcttctggg gcgacctgcc cgcccgcgat cagtgagtga
     2941 tacgggcgtc gacagccctc aatactcgag ggctatcgac gcccgagtgg cgtcagtcgc
     3001 aaccgttgag tgcctccaac aacccgtcga aggcggcctc ggcgttgccg ctcgcggccg
     3061 accgtgccga ctcgacactg cgaacgatcg cgttgacgtc ttgcgacgtc gcgttgcccc
     3121 gtgccatctg gtcgaggttc cggtcgagat cgttgatgtc ttcgcgaacg tcgcgacccg
     3181 tgaaccgcgc caggtcgtcg agcttgtcgt tcgcccggtc gatgtggcgc tgggcttcgt
     3241 cggcgttgtc ggcgtccttg gccttgcaga tctggtcgcc ggcggccttg cccttgcgtt
     3301 cggcccgcgc ctcggtcgag cacgcggcca acgcgaagac cgacacgagc gccagtgcga
     3361 tgagtgcagt gatgcgcagc ttcatgccag caccgcctcg gggtctttgc gtacgtggaa
     3421 ggcagtcacg atctcggcga tgccgcggat gatcgcaccg atgccgatcc acaagatgag
     3481 caacgcggcc gagcggccgg gatagccgac ggcccagatg ccgatgatga tctcgatgac
     3541 gcccgcgacc accgtcatcc accacaggtc gacctcgtgg cggagcgcga tgccgaagac
     3601 gaagtcgacg gatcccttga tgacgaggaa gatgccgatc agcgcggcga ggatcgtgaa
     3661 cgtctggaac ggcgcggtca gcgccgcgat gccgccgagc acgaagaaca caccgagcac
     3721 gatgtgcacc cacttccatc ccgcgacgaa gccggcttcc atgaactcgg cgacgccggc
     3781 gaagagcagc cagaagccgg tcaggtaacc gatgagcacg gccgagtcgt agtcggccga
     3841 gagcacgaac agcccgatga tgatccacag gatcccggtg atgaggagca cccaccacca
     3901 cttgccgact gcaggctccc gcgaatacga gacctccaca gcagtcacgc gcacctcccg
     3961 atacatcccc gcccggttgg atacc
//
LOCUS       DASUGA010000342         5806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_651587, whole genome shotgun sequence.
ACCESSION   DASUGA010000342 DASUGA010000000
VERSION     DASUGA010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651587"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /gene="miaA"
                     /locus_tag="VFX21_06850"
     CDS             complement(<1..672)
                     /gene="miaA"
                     /locus_tag="VFX21_06850"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX5095709.1"
                     /translation="MTRHLALVGPTATGKSAVALAIARADPDVELISLDSMQVYRGMD
                     IGTAKPAPEELADVRHHLVDVADPDEEWSVKRTQELTRAALADIERRDKRAILVGGTG
                     LYVRAVVDALEIPGGDGDVRAQLERETRTDDGLASAYARLQREDPVAAARIEPDNRRR
                     IVRALEVIAVTGRPFSSFGPGLDVYPAPAIDVRLVGLEMPSDELAARIATRVTGMRER
                     GLVAEV"
     gene            complement(669..2054)
                     /gene="miaB"
                     /locus_tag="VFX21_06855"
     CDS             complement(669..2054)
                     /gene="miaB"
                     /locus_tag="VFX21_06855"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX5095710.1"
                     /translation="MTRRFHVRTFGCQMNEHDSERLAGLLVEQGLEPTDDLAEADVVV
                     LNTCCIRENADNRLYGHLGHLKALKEERPDLQIAVGGCLAQKDRGLVAEKAPHVDVVF
                     GTHNLANAPSLLERAVLEGQIVEILEEHEAYPSALPARREHDHAAWVTIQIGCDNSCA
                     FCIVPSVRGSEVSRRLGDIVHEIEDLARDGVAEVTLLGQNVNSYGRDLGAAQYSPKFA
                     DLLRAVDAIDGIDRIRFTSPHPKDLRPETIAAMAECASVCEHLHLPLQSGSDRTLARM
                     HRGYTAERYLARLAAARAAIPDLAVTTDIIVGFPGETDDDFERTLEVVDAAGYDAAYT
                     FVFSPRPGTEAAEMVDDFVPAEIAQDRMRRLVDVVERHALRTHDARVGRDEAVLVEGP
                     SKKDIAVWSGRTRQNKLVHFVPAADAPARAGDLVEVRITSAAPHWLKGDFVRPLQAAR
                     RRRVRIPVSAV"
     gene            2112..2834
                     /locus_tag="VFX21_06860"
     CDS             2112..2834
                     /locus_tag="VFX21_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5095711.1"
                     /translation="MDVTVAAVIVAAVATLVGASIQGSIGFGMNLVTVPALALVLPDA
                     LPATVILIGLPLAVIMFRHEHHALDRDAIGWILAGRVPGTILGAIIVAALTADELKGV
                     IGVFVLFAVAISVFAPPVPMNHPTEFTAGMVAGTTGTAAGIGGPPLALLYQHHEGPTM
                     RSTLAASFLFGTLLSIITLSVAHQMHLADLWLALGLSPLVFAGSRVGRHITRYVDGRV
                     LRPAVLVFAAVSAIVVIVDALN"
     gene            complement(2845..3810)
                     /locus_tag="VFX21_06865"
     CDS             complement(2845..3810)
                     /locus_tag="VFX21_06865"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX5095712.1"
                     /translation="MTSPVRAARWLLAPFWVGIGILLVVVVARFVAWDDRYSLSLLNS
                     ETVWIFLPAYFVAVAAWCFRRWLLAALATVAVVFHVAMLATTVGSTEPVTDAARAAPR
                     LRVVTANVRFDNFDRDALAHELLAADADVVLLQEITPGWVRALSAAGFNAEYPYSVNY
                     PNGAGGQAVYSRLPLLDVQRAMREFWPTYSARIEVGGRAVSLVDVHAIGPPQGMDKHR
                     DTVDSILDVTRSMPEPRVVAGDFNATPYNETMHRFEALGLDSAFERRGRGLDVTWPNG
                     ERLFPPPIQVDHVLVDDGIVVLDVDTLRGTGSDHQPIVVDLAIVK"
     gene            complement(3853..4905)
                     /gene="recA"
                     /locus_tag="VFX21_06870"
     CDS             complement(3853..4905)
                     /gene="recA"
                     /locus_tag="VFX21_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX5095713.1"
                     /translation="MDRDKALDMALGQIEKQFGKGSIMKLGERGHVGIEAIPTGALSL
                     DLALGIGGLPRGRVVEIFGPESSGKSTLAMHVVAEAQRNGGVCAYIDAEHAMDPVYAA
                     AIGVNVDELLISQPDTGEQALEIADMLIRSGALDVLVIDSVAALVPRAEIEGEMGDAH
                     VGLQARLMSQALRKLTGNLNRSRTICVFINQLREKIGVMFGSPETTPGGRALKFYSSV
                     RLDIRRIESIKDGAEVTGNRTRVKVVKNKTAPPFKQAEFDIMYGKGISREGSLLDVGV
                     DLGIVKKSGAWFTYEGEQLGQGRENVKNFLAENLDLMVEISEKIRAASGIDDVAVDPV
                     EAGDVAIDLEDEDADV"
     gene            5154..>5806
                     /locus_tag="VFX21_06875"
     CDS             5154..>5806
                     /locus_tag="VFX21_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5095714.1"
                     /translation="MRPTLRLGRIAGIEVGVHWSLLLIGALLVTALAGDLFPEVAPHS
                     EGSYWAAAIIATALFFVSILAHELSHAVVARRRGQRVGGITLWALGGVAMLKDEARDA
                     KSELLVAAAGPAMSIAVGGGFVALGFGLDAVTANGSLLPAIAFYLGVVNLVLAAFNLL
                     PGAPLDGGRILSGAIWAVRKDHRRAQIAAAKAGRVLGFLLIGVPFVELMLGWGFNPWT
                     "
BASE COUNT          855 a         2030 c         1971 g          950 t
ORIGIN      
        1 gacctcggcg accaggccgc gctctcgcat cccggtgacg cgggtggcga tgcgagccgc
       61 gagctcgtcc gacggcatct cgagcccgac gagacgaacg tcgatggcgg gcgccggata
      121 gacatcgagc ccgggaccga aggacgagaa cggacggccg gtcacggcga tgacttcgag
      181 cgcgcgcacg atgcgacgcc ggttgtcggg ctcgatccgc gcggccgcga ccgggtcttc
      241 gcgctgcaac cgcgcgtacg cgctcgccaa cccgtcgtcg gtgcgcgtct cgcgttcgag
      301 ttgcgcgcgc acgtccccgt ccccgcccgg gatctccaac gcgtcgacga ccgcgcgcac
      361 gtacaagccg gtcccgccga cgaggatggc gcgcttgtcg cggcgttcga tgtcggcaag
      421 tgcggcacgg gtcagttctt gtgtgcgctt gacggaccac tcttcatcgg ggtccgcgac
      481 gtcgacgaga tgatggcgaa cgtcggccaa ctcctcgggc gccggcttgg cggtgccgat
      541 gtccatcccc cggtatacct gcatcgagtc gagcgagatc agctcgacgt cgggatccgc
      601 acgcgcgatc gcgagcgcaa ctgctgactt gccggtggcg gtcggaccga cgagcgcgag
      661 gtgacgcgtc aaacggccga gaccgggatg cggacgcgcc ggcgccgggc ggcttgcagc
      721 ggacgaacga agtcgccctt gagccagtgg ggcgcggcgc tcgtgatgcg cacctcgacg
      781 aggtcaccgg cgcgcgcggg ggcgtcggcg gccggcacga agtgcacgag cttgttctgg
      841 cgggtgcggc ccgaccacac ggcgatgtcc ttcttggacg ggccttcgac cagcaccgct
      901 tcgtcgcgac cgacgcgcgc gtcgtgcgtg cgcagcgcgt ggcgctcgac gacgtcgacc
      961 aggcggcgca tgcggtcttg cgcgatctcg gccggcacga agtcgtcgac catctcggcc
     1021 gcttccgttc ccggacgggg cgagaagacg aacgtgtacg ccgcgtcgta accggccgcg
     1081 tcgacgacct ccagcgtgcg ttcgaagtcg tcgtcggtct cgcccggaaa gccgacgatg
     1141 atgtcggtcg tgaccgcaag atcggggatc gctgctcgcg ccgccgcgag acgcgccaag
     1201 tagcgctccg cggtgtagcc gcggtgcatg cgcgccagcg tccggtcact ccccgactgc
     1261 aacggcaggt gcaagtgctc gcacacgctc gcgcattcgg ccatcgccgc gatcgtctcg
     1321 ggccggagat ctttgggatg cggcgacgtg aagcgaatgc ggtcgatgcc gtcgatcgcg
     1381 tcgaccgcac gcaggaggtc ggcgaacttc ggcgaatact gcgcggcccc gagatcgcga
     1441 ccgtaggagt tgacgttctg gccgagcagc gtgacctcgg caacgccgtc gcgcgcgagg
     1501 tcttcgatct cgtggacgat gtcgcccagg cggcggctga cttcactgcc gcgcacggac
     1561 ggcacgatgc agaacgcaca cgagttgtcg cagccgatct ggatcgtgac ccacgcggca
     1621 tggtcgtgct cacggcgggc cggcaaggcc gacgggtacg cctcgtgctc ctcgaggatc
     1681 tcgacgatct gaccttcgag caccgcccgc tccaggagcg agggcgcgtt cgcgaggttg
     1741 tgcgtgccga agacgacgtc gacgtgcggc gccttctccg cgaccaggcc gcggtccttc
     1801 tgcgccaggc agcccccgac cgcgatctgg aggtccggcc gttcctcctt gagcgccttc
     1861 aggtgaccga ggtggccgta aagcctgttg tcggcgttct cgcggatgca gcacgtattg
     1921 agcacgacga cgtcggcttc cgcgaggtcg tcggtcggct ccaagccctg ttccacgagc
     1981 agcccggcga ggcgctcgga gtcgtgctcg ttcatctggc acccgaacgt gcgcacatgg
     2041 aaccggcgag tcattgcccg ccattctacg accggccccg ggagcgcctc gggccgtagc
     2101 atccgcccct cgtggacgtc accgtggccg ccgtcatcgt cgcggccgtc gcgaccttgg
     2161 tcggcgcgtc gatccagggg tcgatcggat tcggcatgaa cctcgtgacc gtgcccgcgc
     2221 tcgcgctcgt gttgcccgac gcgctgcccg cgacggtcat cctgatcggg ctgccgctgg
     2281 cggtgatcat gttccgccac gagcaccacg cgctcgaccg cgacgcgatc ggatggatcc
     2341 tcgccgggcg ggtgccgggc acgatcctcg gcgcgatcat cgttgctgcg ctgaccgccg
     2401 acgagttgaa gggcgtcatc ggcgtgttcg tgctcttcgc ggttgcaatc agcgtcttcg
     2461 caccgccggt gcccatgaac catcccaccg agttcaccgc cggcatggtt gccggcacca
     2521 ccggtaccgc ggcgggtatc ggcggcccgc ctctcgcgct tctctatcaa caccacgagg
     2581 gaccgacgat gcgctcgacg ctcgcggcgt cgttcctgtt cggcacgctg ctgtcgatca
     2641 tcacgctgtc ggtcgcgcac cagatgcacc tcgccgacct gtggctcgcg ctcggcctct
     2701 caccgttggt gttcgccggc tcgcgcgtcg gccgtcacat cacgcgctac gtcgacggac
     2761 gcgtgctccg ccccgcggtg ctcgtcttcg ccgcggtctc ggcgatcgtg gtgatcgtcg
     2821 atgcgttgaa ctagccgcgt tgcgtcactt gacgatcgcc aagtcgacaa cgatcggttg
     2881 gtggtcgctg ccggtgcccc ggagcgtgtc gacgtcgagg acgacgatgc cgtcgtcgac
     2941 gaggacgtgg tcgacctgga tcggcggcgg gaacaggcgc tcgccgttgg gccacgtgac
     3001 gtcgagacca cgcccgcgcc gttcgaacgc gctgtcgaga ccgagcgctt cgaaccgatg
     3061 catcgtctcg ttgtacggcg tcgcgttgaa gtctccggcg accacgcgcg gctccggcat
     3121 cgaccgggtg acgtcgagga tgctgtcgac ggtgtcgcga tgcttgtcca tgccctgcgg
     3181 cggcccgatc gcgtgcacgt cgaccagcga caccgccctg ccgccgacct cgatccgcgc
     3241 cgaatacgtc ggccagaact cgcgcatcgc gcgctgcacg tcgagcagcg gcagccgcga
     3301 gtacacggcc tgaccgcccg cgccgttcgg gtagttcacc gagtacggat actcggcgtt
     3361 gaagccggcg gccgacaacg cacgtaccca cccgggcgtg atctcctgca acaacaccac
     3421 gtcggcgtcg gcggccagca gttcgtgggc caacgcgtcg cggtcgaagt tgtcgaagcg
     3481 cacgttcgcg gtcacgaccc gcaagcgcgg cgcagcccgc gccgcgtcag tgaccggctc
     3541 cgtcgacccg accgtcgtgg caagcatcgc gacgtggaac acgaccgcga ccgtcgccag
     3601 cgccgccagg agccagcgcc ggaagcacca cgcggcaacc gcgacgaagt acgcgggcag
     3661 gaagatccag accgtctccg agttgaggag gctcaggctg tagcggtcgt cccacgccac
     3721 gaaacgcgca accacgacga ccagcaagat gccaatgccc acccagaacg gcgcgagcaa
     3781 ccaccgcgca gctcggacgg gtgacgtcac ccgcccgagg ctacggagtc atccgaccgt
     3841 tcgtcgttcg ggtcagacgt cggcgtcttc gtcttcgagg tcgatcgcga cgtcgccggc
     3901 ttcgaccggg tcgacagcaa cgtcgtcgat gccggaggcc gcccggatct tctcgctgat
     3961 ctcgaccatg aggtcgaggt tctcggcgag gaagttcttc acgttctcgc ggccctggcc
     4021 gagctgctca ccttcgtagg tgaaccacgc acccgacttc ttcacgatgc cgaggtcgac
     4081 gccgacgtcg agcagcgaac cctcgcggct gatgcccttg ccgtacatga tgtcgaactc
     4141 ggcctgcttg aacggcggcg cggtcttgtt cttgacgacc ttcacccgcg tgcggttgcc
     4201 ggtgacctcg gcaccgtcct tgatcgactc gatgcggcgg atgtcgaggc gcaccgacga
     4261 gtagaacttc agcgcgcgtc cacccggggt ggtctcgggt gagccgaaca tcacaccgat
     4321 cttctcccgc agctggttga tgaagacaca gatcgtgcgg gagcggttga ggttgccggt
     4381 gagcttgcgc aacgcctggc tcatcagacg cgcctgcaag ccgacgtgcg cgtcgcccat
     4441 ctcgccttcg atctcggcgc gaggtacgag cgcggcgacg gagtcgatga ccaacacgtc
     4501 gagcgcaccc gagcggatca gcatgtccgc gatctcgagc gcctgctcac cggtgtcggg
     4561 ctggctgatc aacagctcgt cgacgttcac gccgatcgcg gccgcgtaca ccgggtccat
     4621 cgcgtgctcg gcatcgatgt acgcgcacac cccgccgttg cgctgcgcct cggcgacgac
     4681 gtgcatcgcg agcgtcgact tgcccgacga ctccggaccg aagatctcga cgacacgccc
     4741 ccggggcagg ccgccgatgc ccagtgcgag gtcgagcgag agcgcaccgg tcggtatcgc
     4801 ctcgatgccg acgtggcccc gctcgccgag cttcatgatc gaacccttgc cgaactgctt
     4861 ctcgatctgg ccgagtgcca tgtcgagtgc cttgtcacgg tccaccgtgg acccctctct
     4921 gtgaatgcct ctgtgacggg ctggctggtc gcaacttccg ggggaagctc gtggagcgaa
     4981 cctaacgagc gggtgtgaca gcatcgtaag cgaacaggcg ttcggggtgg tggatatccg
     5041 cggccctggc ccccggcgcg gggcgagcgt catgtgcggg ttcgcgggaa ttggcgcggg
     5101 acggaacgtt gcggcccgcc gtagcgttgt caccgaggaa caaaggaacc cccatgcgac
     5161 cgaccctccg cctcggacga atcgccggca tcgaggtcgg cgtccactgg agcctgctcc
     5221 tgatcggcgc cctcctcgtc accgcgctcg ccggcgactt gttccccgag gtcgcgccgc
     5281 actccgaggg ctcgtactgg gccgcggcga tcatcgccac cgcgctcttc ttcgtgtcga
     5341 tcctcgcgca cgagctctcg cacgcggtcg tcgcgcgccg gcgcggccag cgcgtcggcg
     5401 gcatcaccct gtgggcgctc ggcggggtcg cgatgttgaa ggacgaagcg cgcgacgcca
     5461 aaagcgagct cctcgtcgcg gcggcgggtc ccgcgatgag catcgcggtc ggcggtggct
     5521 tcgtcgcgct cgggttcggg ctcgacgcgg tgaccgcgaa cggcagtctc ctgcccgcga
     5581 tcgcgttcta cctcggtgtc gtcaacctgg tgctcgccgc gttcaacttg ttgccgggcg
     5641 cgcccctcga cggcggccgc atcctgagcg gcgcgatctg ggccgtccgc aaggaccatc
     5701 gccgcgcgca gatcgcggca gccaaggccg gacgcgtcct cggcttcctc ctgatcggtg
     5761 tcccgttcgt cgagctcatg ctcggctggg gcttcaaccc gtggac
//
LOCUS       DASUGA010000343         3892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1064205, whole genome shotgun sequence.
ACCESSION   DASUGA010000343 DASUGA010000000
VERSION     DASUGA010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064205"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFX21_06880"
     CDS             complement(<1..402)
                     /locus_tag="VFX21_06880"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX5095715.1"
                     /translation="MSRDWQPRKFVQRFFLALVVTTVLMGSAVGGAYWYGNDSIDDAT
                     HVDLDLPDGGNFLLIGSDTRAFVSDSADEQHFGDPSEQTGQRSDTIMVAHLDPKTKTG
                     LLVSFPRDLWVDVPGLGHAKINAAFNEGPERV"
     gene            complement(476..2005)
                     /locus_tag="VFX21_06885"
     CDS             complement(476..2005)
                     /locus_tag="VFX21_06885"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX5095716.1"
                     /translation="MAPGTSLRSFFARFAIALVAVVACAGVAVAFGNGEVEDSFNDIE
                     VQPIRPDALDLPDPGGPQNFLVVGTDSRAFVETEGEQQLFGTADEVGGQRSDAIMVVH
                     IDPHAAAGFVVSFPRDLWVDIPGHGSGRLNSAIGYGDASLIIDTINENFHIPINHYLE
                     LDFAGFQKIVDTIGSVNIWFPTPAKDEVSALNIPEAGCRSLNGADALAYVRARHYQWY
                     DAEAGKWRMDPRSDLSRIERQQYFVRSLAQTALDRGARNLTTAFALLDDMSDALKRDP
                     NLTVKDLKALINAFRDVNPADIEMVTLPVDRANKGGADVVVPHEPDADAVLSRLWLGG
                     MPQGLPPMAAPADTTVHVLNGSGEKGRAAEVQQMFEAAGFRTVGEPGDADRDDYPVTQ
                     VRWEPGKGGEALAVVAALGAPSAAEAGPDDELGGADVLVIVGRDWDALAAPVKEGAAA
                     PSTEAPTTGPSGTTTAPATTTTVTTAPPTTPPSTQPPSPSATAVVPVDPETGGTLVGC
                     P"
     gene            2077..3303
                     /locus_tag="VFX21_06890"
     CDS             2077..3303
                     /locus_tag="VFX21_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010472281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEX5095717.1"
                     /translation="MALSPASVLAHLPTTQGCAVVRDGDSTLIGVAPDAVVAARHDTA
                     FAALDAAARGGFWTGYLAYDLGRAVERIPERATDDLEVPDVVLARYDARLVLTPEGDV
                     EIVGDGPTADVLAQAAWRAQRDTAVAWSPRVHRWDSSLDRDQYEAAVARVLDHLAAGD
                     CYQVNLTRRLRTSGAADPIELFAALARSNPAPHAMLCTFGTAGPDVAIVSASPELFLR
                     VDGRRVTTRPIKGTDRDAVRLAASAKDHAENVMIVDLSRNDLGRVCRYGSITVPELCA
                     VEAHPGLYHLVSTVCGELRRDVGFADLVRATFPPASITGAPKPRVMEIIEDLEPVRRG
                     VYCGAVGWIDGDAGRAELAVAIRTFVIARGQTFLGVGGGIVADSSADREWAETELKAA
                     RLLEAASGASAEAGHR"
     gene            3300..>3892
                     /locus_tag="VFX21_06895"
     CDS             3300..>3892
                     /locus_tag="VFX21_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX5095718.1"
                     /translation="MIVWIDGRLTPLAEARVSPLDHGITVGDGVFETMRVYGGVPFAW
                     RRHLDRLAASASGLGLQSPDRAGLRVATDAVLDANGLGAGDARVRITVTGGPTSPGSS
                     RHGSSRTTVFVTATPFEPAAPTADVVIAPWTRNEHGALAGLKTISYGANVRALAYAEA
                     HDAIEAVFANTKMDLCEATGSNVFVVHDDMLCTPPASA"
BASE COUNT          561 a         1374 c         1365 g          592 t
ORIGIN      
        1 gacccgttcg ggaccttcgt tgaacgcggc gttgatcttg gcgtgaccca aaccgggcac
       61 gtcgacccac aggtcgcgcg ggaacgacac gagcaacccg gtcttcgtct tcgggtcgag
      121 atgggcgacc atgatcgtgt cggagcgctg ccccgtctgc tcgctcgggt caccgaagtg
      181 ctgctcgtcg gcgctatcgg agacaaacgc gcgggtgtcg gatccgatca ggaggaagtt
      241 gccgccgtcg ggcagatcga ggtcgacgtg cgtcgcgtcg tcgatgctgt cgttgccgta
      301 ccagtacgcg ccaccgaccg cgctccccat cagcacggtc gtgacgacca gtgcgaggaa
      361 gaagcgctgg acgaacttgc gcggctgcca atccctgctc actgagcgat ccacggtagt
      421 gacgggaccg ccgctcccga cggatacatg accctgggca ccatgaccac gagaactacg
      481 ggcagccgac caaagttccc ccggtctcgg ggtcgaccgg caccacggcg gtggccgagg
      541 gcgagggcgg ctgggtcgag gggggcgtgg tcggtggggc cgtcgtcacg gtcgtggtgg
      601 tagccggggc cgtggtggtg cccgacgggc cggtggtcgg ggcctcggtg gacggcgccg
      661 ccgcaccctc cttgaccggc gcggccagcg catcccagtc gcggccgacg atcacgagca
      721 cgtcggcgcc gccgagctcg tcgtcgggac cggcttcggc cgcactcggc gcgcccagcg
      781 cggcgacgac cgcgagagcc tcgccgccct taccgggctc ccagcggacc tgcgtgaccg
      841 ggtagtcgtc gcggtctgcg tcgccgggct cgccgacggt ccggaagccg gcggcctcga
      901 acatctgctg cacctccgcg gcgcggccct tctcgcccga tccgttgagc acgtgcacgg
      961 tcgtgtcggc gggcgccgcc atcggtggca ggccctgcgg cataccgccg agccacaggc
     1021 gcgaaagcac cgcgtcggca tcgggttcgt gcgggacgac gacgtccgcg ccgcccttgt
     1081 tcgcgcggtc gacgggcaac gtcaccatct cgatgtccgc agggttcacg tcgcggaacg
     1141 cgttgatcaa cgccttgagg tccttgacgg tcaggttcgg gtcacgcttg agcgcgtccg
     1201 acatgtcgtc gagcagcgcg aacgcggtcg tcaagttccg cgcgccgcgg tcgagggcgg
     1261 tctgcgccag actgcgaacg aagtactgct ggcgctcgat gcggctgagg tcgctgcgcg
     1321 ggtccatgcg ccacttgccc gcctcggcgt cgtaccactg gtagtgacgc gcgcgcacat
     1381 acgcgagcgc atccgccccg ttcaggctgc ggcaacccgc ttcggggatg ttgagtgccg
     1441 acacttcgtc ttttgccggc gtcgggaacc agatgttcac cgagccgatc gtgtcgacga
     1501 tcttctggaa accggcgaag tcgagttcga ggtagtggtt gatcgggatg tggaagttct
     1561 cgttgatcgt gtcgatgatc aacgacgcgt cgccgtatcc gatcgcactg ttcaaccggc
     1621 ccgagccgtg cccgggaatg tcgacccaga gatcacgggg aaaggagacg acgaacccgg
     1681 ccgccgcatg cgggtcgatg tgcacgacca tgatcgcgtc cgaccgctgg ccgccgactt
     1741 cgtcggcggt cccgaagagc tgctgctcac cttcggtttc gacgaacgcg cggctgtcgg
     1801 tgccgacgac gagaaagttc tgcggaccgc cgggatcggg caaatcgaga gcatcgggtc
     1861 ggatcggttg cacctcgatg tcgttgaagc tgtcttcgac ttcaccgttc ccgaacgcga
     1921 ccgccacacc ggcgcacgcg acgactgcca cgagcgcgat cgcgaagcgc gcgaagaacg
     1981 aacgaagaga ggtgcccgga gccaagccgt gggagtttac cgaccgtccg attttgcgca
     2041 cggtcggctc tcgcggtgcg ggtaccgtcg cgagcgatgg ccctttcgcc cgcttcggtc
     2101 ctggcgcacc tgcccacgac gcagggctgc gcggtggtgc gcgacggtga ctccacgctc
     2161 atcggcgtcg cgcccgacgc ggtcgtcgcg gcgcgccacg acaccgcatt cgcagcgctc
     2221 gatgccgcgg cgcgcggcgg cttctggacc ggctacctcg cctacgactt ggggcgcgcc
     2281 gtcgagcgca tccccgagcg cgcgaccgac gatctggagg tccccgacgt cgtgctcgcg
     2341 cgctacgacg cgcgactcgt cctcactccc gagggcgacg tggagatcgt cggcgacgga
     2401 ccgacggccg acgtgctcgc gcaggcggca tggcgcgcgc agcgcgacac cgccgtcgcg
     2461 tggagcccgc gcgtgcaccg ctgggactcc tccctcgatc gcgaccagta cgaagccgcg
     2521 gtcgcgcgtg tgctcgacca cctcgccgcg ggcgactgct accaggtcaa cctcacgcgg
     2581 cgcctgcgca cttccggcgc ggccgacccg atcgagctgt tcgccgcgct cgcgcgctcg
     2641 aaccccgcgc cgcacgcgat gttgtgcacg ttcggtaccg ccggtcccga cgtcgccatc
     2701 gtctccgcgt caccggagct cttcctccgc gtcgacggcc gccgggtgac gacgcgcccg
     2761 atcaagggca ccgaccgcga cgccgtgcgg ctcgcggcca gcgccaagga tcacgccgag
     2821 aacgtgatga tcgtcgacct cagccgcaac gacctcggtc gcgtctgccg ttacgggtcg
     2881 atcaccgtgc cggagctgtg cgccgtcgaa gcccacccgg gcctctacca cctggtgtcg
     2941 acggtgtgcg gcgagttgcg ccgcgatgtc ggtttcgccg atctcgtgcg ggcgacgttc
     3001 ccgcccgcgt cgatcaccgg cgcgcccaag ccccgtgtca tggagatcat cgaagacctc
     3061 gaaccggtgc gccgcggcgt gtactgcggc gcggtcggat ggatcgacgg cgacgcgggc
     3121 cgggccgagc tcgcggtcgc gatccgcacg ttcgtcatcg cgcgcggcca gacgttcctc
     3181 ggcgtgggcg gcggcatcgt cgccgactcc agcgcggatc gggaatgggc ggagacggag
     3241 ctgaaggcgg cgcggctgct cgaagccgcg tcgggcgcga gcgcagaggc cgggcaccgg
     3301 tgatcgtctg gatcgacggg cggctcaccc cgctcgccga agcgcgcgtg tcaccgctcg
     3361 accacggcat cacagttggc gacggcgtct tcgagaccat gcgcgtctac ggcggcgtgc
     3421 cgttcgcgtg gcggcgccac ctcgaccggc tcgccgcatc cgcgtccggg ctcggcctgc
     3481 agtcacccga ccgcgccggc ctgcgcgtcg cgaccgatgc cgtgctcgac gccaacggac
     3541 tcggcgcggg cgacgcgcgc gtgcgcatca cggtcaccgg agggccgaca tcgcccggtt
     3601 cgtcgcggca cggcagctcg cgcaccactg tgttcgtcac ggccacaccg ttcgagccgg
     3661 ccgcgccgac tgccgatgtc gtgattgccc cgtggacgcg caacgagcac ggggccctcg
     3721 cgggcctgaa gaccatctcg tacggcgcga acgtacgcgc cctcgcgtac gccgaggccc
     3781 acgatgcgat cgaagccgtg ttcgcgaaca cgaagatgga cctgtgcgag gcgacgggct
     3841 cgaacgtgtt cgtcgtgcac gacgacatgc tctgcacgcc gccggcgagt gc
//
LOCUS       DASUGA010000344         2403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_461037, whole genome shotgun sequence.
ACCESSION   DASUGA010000344 DASUGA010000000
VERSION     DASUGA010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461037"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VFX21_06900"
     CDS             <1..742
                     /locus_tag="VFX21_06900"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX5095719.1"
                     /translation="ATARRIVDALLESDAGLVEQWPALRDRLPATARDAADGLVTALT
                     PLEGELVVSHAERLRAALAPLVEQNYDDAAARLCDLDALVDVARRAERLADVATELTL
                     EPPQSSGDLAGPPMIDEDWLVISTVHSAKGLEWDVVHVLHATDGNFPSDMALTSNDGL
                     EEERRVFYVGVTRPRHSLHVYVPLRFHVRPHHRDDAHMYGQPSRFLTERVTQHFDRDA
                     AGHDEPAMAAAATATATRVELSLDALWS"
     gene            complement(739..1242)
                     /locus_tag="VFX21_06905"
     CDS             complement(739..1242)
                     /locus_tag="VFX21_06905"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEX5095720.1"
                     /translation="MHLELVQGDITRQAVDAIVNAANSSLLGGGGVDGAIHRAAGPEL
                     LHECRLLGGCPTGEARVTRGYRLPATWIIHTVGPVWRGGQQGEPDLLSACYRNSFRVG
                     VEVGARSLAFPAISTGIYGYPSDLGARTAVREAKRFVGPIDLVRFVAFDGVTFAAYEQ
                     ALAEPVD"
     gene            complement(1260..>2403)
                     /locus_tag="VFX21_06910"
     CDS             complement(1260..>2403)
                     /locus_tag="VFX21_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX5095721.1"
                     /translation="TKFEGEIIAMTLDLLHADAITGTEPAGMVTSGGSGSILHAVLAY
                     REAAAARGITQPNFVKPETGHPAFDKACHLLGIELRRAPIDPKTATADVDAVAERVDG
                     NTIAIMGSACCYGYGTVDPIGALSDLALERGVGLHVDGCLGGWILPFGQELGYDIPLF
                     DFRIPGVTSISADTHKYGYGFKGSSVLAFRDKALRNGQYFFITDWTGGKYASPGIDGS
                     RSGGLIAATWAALVSIGRTGYLGYARQIFETAFAMQDAVRSHPELRIIGSPTFCFSFT
                     SDDFDIYHVNDAMRQRGWRFNGQQYPNAIHMAVTRPQTQPGLVEAFTADLAEAVQYAR
                     EHKGEKPSSGAIYGGVEGGMTDEADEFIRSVMTDMLDKHQGLPRPE"
BASE COUNT          368 a          849 c          811 g          375 t
ORIGIN      
        1 ggcgactgcg cgccgtatcg tcgacgcgct cctcgagtcg gatgccgggc tggtcgagca
       61 gtggccggcg ctgcgcgacc gcctgccggc cacggcgcgc gacgccgcgg acgggctcgt
      121 caccgcgctc acgcccctcg aaggcgaact cgtcgtctca cacgcggaac gactgcgggc
      181 cgcgctcgcg ccgctggtcg agcagaacta cgacgacgcc gcggcgcggc tgtgtgatct
      241 cgacgcgctc gtcgacgtcg cccggcgcgc cgagcgcctc gccgacgtcg cgaccgagct
      301 cacgctcgaa ccgccgcagt cgagcggcga cctggccggg cccccgatga tcgacgaaga
      361 ctggctggtc atctccactg tccactcggc caagggcctc gagtgggacg tcgtgcacgt
      421 gctgcacgcc accgacggca acttcccgtc cgacatggcg ctcacctcga acgacgggct
      481 cgaagaggaa cggcgcgtct tctacgtcgg cgtcacccgc ccgcggcact cactgcacgt
      541 ctacgtgccg ctgcggttcc acgttcgccc gcaccaccgc gacgacgcgc acatgtacgg
      601 tcagccgtcg cgcttcctca ccgaacgggt cacgcagcac ttcgaccgcg acgccgccgg
      661 ccacgacgaa ccggcaatgg cggcggctgc gacggcgacc gcaacgcgag tggagctgtc
      721 gctcgacgcg ctgtggtcct agtcgactgg ttcggcgagt gcctgttcgt aggccgcgaa
      781 cgtgacgccg tcgaacgcaa cgaaccgaac gagatcgatc ggaccgacga aacgctttgc
      841 ctctcgcacg gcggtgcgtg cgcccaagtc ggacgggtag ccgtatatgc cggtcgagat
      901 cgccgggaac gcaagcgagc gggcaccgac ctcgacgccg acgcgaaacg agttgcggta
      961 gcacgcggac aacaggtccg gctcgccctg ttgaccgccg cgccaaaccg gtccgaccgt
     1021 gtgaatgatc cacgttgccg gcagccggta gccgcgtgtg actctcgctt cgccggtggg
     1081 gcacccaccg agcagccggc actcgtgcaa gagctcgggc ccggccgcgc ggtgaatggc
     1141 gccgtcgacg ccaccgccgc cgagcagcga cgagttcgcg gcgttcacga ttgcgtcgac
     1201 cgcctgtcgc gtgatgtcgc cctgcacgag ctcgaggtgc atacgtcgtc gacctcgcgt
     1261 cactccgggc gcggcaggcc ttggtgcttg tcgagcatgt cggtcatcac gctgcggatg
     1321 aactcgtcgg cctcgtcggt catgccgcct tcgacgccgc cgtagatcgc gccactcgac
     1381 ggcttctcac ccttgtgctc gcgcgcgtac tgcaccgcct cggcgagatc ggccgtgaac
     1441 gcctcgacca ggcccggctg cgtctgcgga cgcgtcaccg ccatgtggat cgcgttcggg
     1501 tactgctggc cgttgaagcg ccaacccctt tggcgcatcg cgtcgttgac gtggtagatg
     1561 tcgaagtcgt cgctggtgaa gctgaagcag aacgtcgggc tcccgatgat gcgcagctcg
     1621 gggtgcgagc gcaccgcgtc ttgcatcgcg aatgcggtct cgaagatctg ccgcgcgtaa
     1681 ccgaggtatc ccgtgcggcc gatgctgacg agcgcggccc acgtggccgc gatgagtcca
     1741 cccgaccgtg acccgtcgat gccgggcgac gcgtacttgc cgccggtcca gtcggtgatg
     1801 aagaagtact gaccgttgcg cagcgccttg tcgcggaacg cgagcaccga cgaacccttg
     1861 aagccgtatc cgtacttgtg cgtgtcggcg gagatcgacg tcacgcccgg gatgcggaag
     1921 tcgaacagcg ggatgtcgta accgagctcc tggccgaacg gcaggatcca tccgcccagg
     1981 caaccgtcga cgtggagccc gacgccgcgt tcgagcgcga gatcggagag cgcgccgatc
     2041 gggtcgacgg tgccgtaccc gtagcaacac gccgaaccca tgatcgcgat cgtgttgccg
     2101 tcaacgcgct cggcgaccgc gtcgacatcg gcggtcgccg tcttcgggtc gatcggtgcc
     2161 cgccgcagct cgatgccgag caggtggcac gccttgtcga acgcggggtg cccggtctcg
     2221 ggcttcacga agttcggttg cgtgataccg cgggccgccg cggcctcgcg gtacgcgagc
     2281 accgcgtgga ggatgctgcc gctcccgccc gatgtcacca tgccggccgg ttcagtgccg
     2341 gtgatcgcgt ccgcgtgcag caggtcgagc gtcatcgcga tgatctcgcc ctcgaacttc
     2401 gtc
//
LOCUS       DASUGA010000345         2085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_983298, whole genome shotgun sequence.
ACCESSION   DASUGA010000345 DASUGA010000000
VERSION     DASUGA010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983298"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VFX21_06915"
     CDS             <1..229
                     /locus_tag="VFX21_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020490044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5095722.1"
                     /translation="LPDDRCQCPHWGVVLKGKVKFTTDEGETVIEAGDAYYIPPGHLP
                     YLYAGTEVIEFSPTHELNETLAVIEKNMANA"
     gene            complement(345..797)
                     /locus_tag="VFX21_06920"
     CDS             complement(345..797)
                     /locus_tag="VFX21_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095723.1"
                     /translation="MNTHTVLLTLHIAAIASWLGADVLLYAMRHRWKRETPEATKAWA
                     RMQFWLHDRYYAVVALLILVTGIALVQHEHWGWSSKFIWVGLAAIAAGATLGGIGLKG
                     LAKQRVEALDASDADTAVATDRRALPIELLLTAFVLITVAAMVHKWGA"
     gene            complement(807..1487)
                     /locus_tag="VFX21_06925"
     CDS             complement(807..1487)
                     /locus_tag="VFX21_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004973527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEX5095724.1"
                     /translation="MRLNIEAGPAFAFGTITIDSGGELHVEAGAMAAYSDGVEVETKA
                     KGGVLAGLKRSVLGGESFFINTFRATSGGTVSVAPALPGDLSVVPVDGSQALMVQSGS
                     WIASDPGIQVDTKWGGAKTFFSGEGLFLLRCTGQGDVLMSSYGAILTMTLEPGQSHTL
                     DTGHVVAFDESVQYQVSKAGNWKTTILGGEGFVTRFTGPGRLWLQTRSSQDLLDWLIP
                     KLPTKSSS"
     gene            complement(1541..2053)
                     /locus_tag="VFX21_06930"
     CDS             complement(1541..2053)
                     /locus_tag="VFX21_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX5095725.1"
                     /translation="MSAHRRKATSAEVRALAHPLRLRIIRLLYDADRSNKELADRLHV
                     DPATVLHHVRTLEKTGFLKAAGTRPGARGSVEKLYRSTGKSWKIDVDAGLPGASRVSQ
                     ATMDAFLAELQAAGPDADLSTARLALNINEARREELMARLGAVLDEYALDDDADGEPW
                     ALFVAVHRRG"
BASE COUNT          386 a          680 c          657 g          362 t
ORIGIN      
        1 gctgcccgac gaccgctgcc agtgcccgca ctggggagtc gtgctcaagg gcaaggtgaa
       61 gttcacaacc gacgaaggcg agacggtgat cgaagccggc gacgcgtact acatcccgcc
      121 ggggcacctt ccctatctgt acgcaggcac cgaagtgatc gagttcagcc caacgcacga
      181 actcaacgag acgttggccg tcatcgagaa gaacatggcc aacgcctgag cggcgtggcg
      241 gtggatatcc cggcgggatc gagtacccgc cgggatacac ggaagaaatc ctttgaaata
      301 ttgctggatt cctcggttgt tgtccgaggt ctccgtcggc gagatcaggc tccccacttg
      361 tggaccatcg ccgcgaccgt gatgagcacg aacgccgtca gcaagagctc gatcggcagg
      421 gcgcgtcggt cggtagcaac tgcggtgtct gcgtcgctcg cgtcgagcgc ctcgactcgc
      481 tgcttggcca agcctttcaa tccgatgcca ccgagcgtcg cgcccgcggc gatcgcggcg
      541 agcccgaccc aaatgaactt cgatgaccac ccccaatgct cgtgctgcac cagcgcaatg
      601 ccggtaacca ggatgagcag ggcgacgacc gcgtaataac ggtcgtgaag ccagaactgc
      661 atccgagccc aggccttcgt cgcttcaggc gtctcccgct tccaccggtg acgcatcgcg
      721 tatagaagga cgtcggcacc gagccatgac gcgatcgcgg cgatgtgcag tgtgagcaac
      781 accgtgtggg tgttcacggc gcgaccctaa gaactcgact tggttgggag cttggggatc
      841 agccagtcga ggaggtcttg gctgctgcga gtctgcagcc acaggcgacc gggcccggtg
      901 aagcgcgtga cgaatccttc gccgccgagg atggtggtct tccagttgcc cgccttcgac
      961 acttggtact gcacggactc gtcgaacgcg acgacgtgcc cagtgtcgag ggtgtggctc
     1021 tggcccggct cgagcgtcat cgtgaggatg gcgccgtaac tcgacatcaa cacgtcgcct
     1081 tggccggtgc aacgcaggag gaacaagcct tcaccactga agaacgtctt cgcgccgccc
     1141 catttggtgt cgacctgaat gcccggatcg gatgcgatcc acgaacccga ctgcaccatg
     1201 agtgcctgcg aaccgtcgac cggtacgacc gaaaggtcgc cgggcaacgc cggcgcgacg
     1261 ctgacggtgc ccccgctcgt cgcccggaac gtgttgatga aaaagctttc accgccgagg
     1321 accgagcgct tcaaaccggc aagcacgccg cccttcgctt tcgtctcgac ctcgacgccg
     1381 tcggagtacg ccgccatcgc gccggcttcg acatgcagtt caccgccgga atcgatggtg
     1441 atagtgccga aggcaaacgc aggacccgct tcgatgttca atcgcatgcg cggaagcgta
     1501 cggcgaggtt cgtaccgcga gggtgatgac ccttggacct ttacccgcgg cgatgaaccg
     1561 ccacgaacaa cgcccacggc tcaccgtcgg cgtcgtcgtc gagcgcgtac tcgtcgagca
     1621 ccgcgcccag ccgcgccatg agttcttcac ggcgggcctc attgatgttc agcgcgaggc
     1681 gcgcggtcga gagatcggcg tccgggcccg cggcttggag ttctgcgagg aaggcgtcca
     1741 ttgtcgcctg actgacgcgg ctcgcgcccg gcaagccggc gtcgacatcg atcttccacg
     1801 actttcccgt cgagcggtac agcttctcga cgctgccgcg cgcgcccggc cgggtgcccg
     1861 cggccttgag gaatcccgtc ttttccaacg tccgcacgtg atggagaacg gtggctgggt
     1921 caacgtgcag ccgatcggcc agttccttgt tcgaccgatc tgcgtcgtag agcagacgga
     1981 tgatccgcag tcgtagcgga tgcgcgagcg cacgcacctc ggccgacgtc gcttttctcc
     2041 tgtgcgcact cataaaccga cgctcaccct agcaagtgat tgggt
//
LOCUS       DASUGA010000346         8446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1146107, whole genome shotgun sequence.
ACCESSION   DASUGA010000346 DASUGA010000000
VERSION     DASUGA010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8446
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146107"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1562)
                     /locus_tag="VFX21_06935"
     CDS             complement(120..1562)
                     /locus_tag="VFX21_06935"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5095726.1"
                     /translation="MSIQGVVRDRIGRGRGLEVAETTPRHRVVIVGGGFGGLPACRFL
                     ANLPVDVTLIDRRNHHLFQPLLYQVATGILSPGQIAIPLRQVVRKAKNVTVEQAEVTD
                     VDLERRVVKARRLPGLEIEVPYDSLIVAAGAGQSYFGHDEFALHAPGLKTIDDALELR
                     RRILGAFELAETAPNDEERRFWLTIVVVGAGPTGVEIAGQIRELATRTLRDNFRAFDP
                     ASVRVLLVDGGKEPLATFGDDLSQRAAQELESLGVELKMGARVTNVDGAGATVQTDDG
                     TESVSAGTVIWAAGVQASPLAEMLAKASGAEVDRAGRIATRPDLTLPDHPEVFAVGDM
                     VTLNDLPGVAEVAMQGGLHAANTIVRRLRGEHEAKPYKYHDLGSVAAIGRFRAICSVG
                     RLRLSGFAGWFVWMFVHLGFLNGFGNRATTVLRWCRWLVGRQRVERVFSVAHTGGDLS
                     APAAVRSVIEPNPLPGLTDSAIDPAGKRTP"
     gene            complement(1559..3406)
                     /locus_tag="VFX21_06940"
     CDS             complement(1559..3406)
                     /locus_tag="VFX21_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX5095727.1"
                     /translation="MTDTYPAIAEHGLIGDLQTCALVATDGTIDWFCAPRFDAPSVFA
                     SLLDANRGGSFSLRPARADYVAKQLYFPDTAVLITRFLSSEGVAEIADFMPVDHPGQP
                     TDRHSIVRLVRVPRGEVNLQFEVAPRFDYGRGDHTVNMTEHGVVFDSGKFALTLHSST
                     ALEQRGKDAGGVLTLKAGEIGGFILETQADGPPRHVTADELDRCFRKTVHVWRSWLSA
                     STYQGRWRDMVNRSAITLKLLTYAPSGAPIAAATAGLPEQVGGERNWDYRYTWLRDGS
                     FSVQALARIGFLDEAYGFLAWLRDRMEERNEGPSGPMQIMYRVDGSSDLVEYELDNLE
                     GYRGSAPVRIGNAASEQLQLDTYGEMLDAIYHAERDEPIIGYRGWTDLCSVIDWVCEN
                     WDRDEEGIWETRGGRKPFVYGRMMCWAAIDRAIRMATLTGSRPAPIERWTACRDAIHE
                     QIVTRGWNPELHALVQHYGSDVLDSSVLAALRLHYLVPTDELWLGTLHAIEDKLVSDS
                     LVYRYNPEASPDGLRGSEGTFSMCTFWYVDALTRTNRLDDARLVFEKMLTYGNHLGLY
                     AEEIGDNGEQLGNFPQAFTHLSLINAAVDLDRRLDRQSGHTPSSLPFLQ"
     gene            3572..4339
                     /locus_tag="VFX21_06945"
     CDS             3572..4339
                     /locus_tag="VFX21_06945"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX5095728.1"
                     /translation="MTLEGKVAIVTGGNSGIGKAVTLALAEKGASIVIDYVANEKATE
                     ELEQQIAKLGDQSIGVEADVSKVEDLETLIEQAVKAFGRIDVMVNNAGIETRSSVLDT
                     SEDQYDRVLAINLKSAFFGTQIAAKQMIAQGGGGTIINITSVHEDWPMPGNTAYCLSK
                     GGMRMLTRTAGVELGVHGIRVIGVGPGAVATPINTQTMNDPAAMKTLDNAIPLGRMAQ
                     PHEIGQVVAFLASDGASYMNATTVFADGGLMHSSPGL"
     gene            4349..5902
                     /locus_tag="VFX21_06950"
     CDS             4349..5902
                     /locus_tag="VFX21_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020104785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX5095729.1"
                     /translation="MPERYDVIIIGTGAGGGTLAHTIAPSGKKVLILERGNFLPREME
                     NWTPAPVFIDGRYISEDTWYDASGKAFQPQVHYFVGGATKLYGAALYRLRPQDFGEVK
                     HVDGVSPEWPLTYDDFEPYYSKAEWLYQVHVNHGEDPTEGHLSKQYPWPAVSHEPRIQ
                     QVADDLEKAGYHPFHAPCGVLLDEAERAKSTCIRCTWCDGYPCLVHAKSDAETIAVRP
                     IIDLPNVTLLVDTEVVQLATNASGRAVTGVVVSRGGEREVYEADIVVLSAGASNSAKI
                     LLASAGDQHPNGLANGSDQVGRNYMFHNSKAVVALSKERNDTTFQKTLGLNDFYFGAD
                     DYTWPIGNIQMVGKSNAEAMKGEEPKLTKLAPHWSLDDVAHHAVDFWLTTEDLPLPEN
                     RVTIDGDRNVHLAYKSTNDEEAGRLYHELRKILNHVGMADHHVLDKNFYMDMSIPVAG
                     CAHQAGTCRFGRDASTSVLDENCRAHEVDNLYVVDTSFFPSIGAVNPALTAMANAIRV
                     GEHLLERMS"
     gene            5929..7167
                     /locus_tag="VFX21_06955"
     CDS             5929..7167
                     /locus_tag="VFX21_06955"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5095730.1"
                     /translation="MATETEQRVVQIAAVAQGVALVTFPAASTIFTNADDYDLSRSAY
                     GAMFVPQAVTAVAASVLGASWAARVGTRKIYLIGLTANVVAMALLVVSATVQHDESIA
                     YPILLLATGCLGVGFGFTVPALNTFTAAFNPAKVDASVLVLNALLGVGTALAPVLVAV
                     FVGLDLWWGLPALTGIVLVGLLVASSRLAFAVDSSARPPEATARRSIPVRFWIYAGFA
                     LCYGICETMNGNWASLDMKDLGASTTQASLALTTFWASVTLGRVVFAALQRWVPTSLI
                     YRALPVLLVAVFLAISQLPDDEPALAIVTFGLAGLGCSALLPLTISFGQGELTAMSAG
                     VAGGVIASYQVGYGIAAFGAGPLQSAGVDLAALFAWSAVVAAVMAALALVLTRSRADG
                     LADLGARRELPSTRGRRTLN"
     gene            complement(7164..7556)
                     /locus_tag="VFX21_06960"
     CDS             complement(7164..7556)
                     /locus_tag="VFX21_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX5095731.1"
                     /translation="MLGEWQVGEVLWTMIWFTLFFIWIYLLIIVFSDIFRSHDLGGFA
                     KFLWVIFIIFLPYLGVFVYLIARGHKMSEHAMADAQKADEAARAYIRDAAGSGGSSAD
                     ELARLADLKAKGVIDDAEFAKMKAKIVG"
     gene            complement(7718..>8446)
                     /locus_tag="VFX21_06965"
     CDS             complement(7718..>8446)
                     /locus_tag="VFX21_06965"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX5095732.1"
                     /translation="FRGVLGEQFANNFTGLVGGTVATTALVIRFFQKQGQKIAVAASS
                     GILNSFAGFVVQTFVIIIGLAVTGDEFIPSSTGGDRTIAGVVILVIIVGGIALALALV
                     VPKLRRRLKGLIAPQWDAAKENLRLILSTPRKAVMLFGGNLASQILYALVLDASLHAY
                     GQDLPVLQLMVVNSLASLLGGMAPVPGGMGVVEAGMIAGLTAAGIPEAQAVAATFTHR
                     LFTAYLPPIWGWFSLQWLRRNDYV"
BASE COUNT         1474 a         2793 c         2820 g         1359 t
ORIGIN      
        1 cacggtctcg cgccggtgtg cgctgggaac cgtgagcttc agctcgacgc tgtcggcctc
       61 tttgatgagc gcgagcatct cgatcaggcg gtcgcccgag atcggttcgt gggtcgtcgt
      121 catggtgtcc tctttcccgc cggatcgatc gcactatcgg tgagcccggg gagcggattc
      181 ggttcgatga cggaacgtac cgcggccggt gcgctcagat cgccccccgt gtgcgccacg
      241 ctgaagacgc gctcgacccg ctgacgcccc acgagccacc ggcaccaccg cagcaccgtc
      301 gtcgcgcgat tgccgaaacc gttgaggaag ccgaggtgca cgaacatcca cacgaaccaa
      361 ccggcgaagc cgctcaaccg gagccggccg acactgcaga tcgcgcggaa gcgcccgatc
      421 gcggcgacgc tgccaaggtc gtggtacttg tacggcttcg cctcgtgctc gccgcgcaac
      481 cggcgcacga tcgtgttcgc cgcgtgcaga ccgccttgca tcgcgacctc ggcgacaccc
      541 ggcagatcgt tcagcgtgac catgtcaccg accgcgaaga cctcgggatg gtcgggcagc
      601 gtgaggtcgg gccgcgtcgc gatgcgtccg gcccggtcga cctccgcgcc cgatgccttc
      661 gcgagcatct cggcgagcgg cgacgcctgc acacccgcgg cccagatgac ggtgcccgca
      721 ctgacggact ccgtgccgtc atcggtctgc acggtggcgc cggcaccgtc gacgttcgtg
      781 acgcgcgcac ccatcttcag ctcgacgccg agcgactcga gctcctgcgc cgcgcgctgc
      841 gagaggtcgt caccgaaggt cgcgagcggc tccttgccgc cgtcgaccaa gagcacgcgc
      901 accgacgcgg gatcgaacgc gcggaagttg tcgcgcagcg tccgcgtcgc gagctcgcgg
      961 atctgaccgg cgatctcgac gccggtgggc ccggcgccga cgacgacgat cgtgagccag
     1021 aaccggcgtt cttcgtcgtt cggcgcggtc tccgcgagct cgaacgcgcc gaggatgcgg
     1081 cgccgcagct cgagcgcgtc gtcgatcgtc ttcagcccag gcgcatgcag cgcgaactcg
     1141 tcgtgcccga agtacgactg cccggcaccg gcggccacga tcaagctgtc gtacggcact
     1201 tcgatctcga ggccgggcaa tcgccgcgcc ttgacgacgc gccgctcgag atcgacgtcg
     1261 gtgacctccg cctgctcgac cgtgacgttc ttcgccttgc gcaccacttg ccgcaacgga
     1321 atcgcgatct ggccgggcga gaggatgccc gtcgcgacct gatacagcag gggctggaac
     1381 aggtggtgat tgcggcggtc gatcagcgtg acgtcgaccg ggaggttcgc gaggaagcga
     1441 cacgcgggca gcccgccgaa cccgccgccg acgatcacga cccgatggcg cggcgtcgtc
     1501 tcggcaacct ccagcccgcg accccgcccg atgcgatcgc gtacgacgcc ctggatgctc
     1561 actggaggaa cggtagcgaa ctcggcgtgt gtcccgactg ccgatccagg cgccggtcga
     1621 ggtcgaccgc ggcgttgatc aacgagagat gagtgaacgc ctgcgggaag ttcccgagct
     1681 gctcgccgtt gtcgccgatc tcttccgcgt agaggccgag gtggtttccg taggtgagca
     1741 tcttctcgaa cacgaggcgc gcgtcgtcga gccgattagt gcgcgtcaac gcgtcgacat
     1801 accagaacgt gcacatcgaa aacgtgccct cggaaccacg cagcccgtcg ggcgaggctt
     1861 ccgggttgta gcggtagacg aggctgtcgg aaacgagctt gtcctcgatc gcgtgcagcg
     1921 tgccgagcca cagctcgtcg gtggggacga gatagtgcag ccggagcgcg gcgagcaccg
     1981 acgagtcgag cacgtcggag ccgtagtgct gcaccagcgc gtggagctcc gggttccaac
     2041 cacgcgtgac gatctgttcg tggattgcgt cgcggcatgc cgtccatcgc tcgatgggtg
     2101 cgggacgcga gcccgtcagg gttgccatcc ggatggcccg gtcgatcgcg gcccagcaca
     2161 tcatgcgacc gtagacgaac ggcttgcgtc cgccgcgcgt ctcccagatg ccttcttcgt
     2221 cgcggtccca gttctcgcag acccagtcga tgaccgaaca gagatcggtc cagccccgat
     2281 agccgatgat gggttcgtcg cgttcggcgt ggtagatcgc gtcgagcatc tcgccgtagg
     2341 tgtcgagctg cagctgctcc gacgccgcgt tcccgatgcg caccggagcc gagccgcgat
     2401 acccctcgag gttgtcgagc tcgtattcga cgaggtccga actgccgtcg acgcggtaca
     2461 tgatctgcat cgggcctgac ggtccttcgt tgcgctcctc catgcgatca cgcagccacg
     2521 cgaggaaccc gtatgcctcg tccaagaatc cgatccgggc gagcgcctgc acgctgaagg
     2581 atccatcccg cagccacgtg tagcggtaat cccagttgcg ctcaccgccg acttgctcgg
     2641 gcagcccggc agtcgcggcc gcaatcggcg cgccgcttgg tgcgtacgtg agcaacttca
     2701 gagtgatcgc ggagcggttc accatgtcgc gccaacgacc ctgatatgtc gatgcggaga
     2761 gccagctgcg ccacacgtgc acggtcttgc ggaagcagcg gtcgagctcg tcggcggtca
     2821 cgtggcgcgg cgggccgtcg gcctgcgttt cgaggatgaa accgccgatc tcgccggcct
     2881 tgagggtgag gacaccgccg gcgtccttgc cgcgctgctc gagggcggtc gaggagtgca
     2941 acgtgagcgc gaacttgccg ctgtcgaaca cgacgccgtg ctcggtcatg ttcacggtgt
     3001 gatcgccgcg cccgtaatcg aagcgcggcg caacctcgaa ctggagattg acctcaccac
     3061 gcggcacgcg cacgagccgg acgatcgagt gccggtcggt cggctgcccg ggatgatcga
     3121 cgggcatgaa gtcggcgatc tcggcgacgc cctcggacga gaggaagcgc gtgatcagca
     3181 ctgcagtgtc ggggaagtac agctgcttgg cgacgtaatc cgcgcgggcg ggcctcaggc
     3241 tgaacgagcc acctcggttc gcgtcgagga gtgacgcgaa cacgctcggc gcgtcgaagc
     3301 ggggcgcgca gaaccagtcg atggttccgt cggtcgcgac gagcgcgcac gtctgcaggt
     3361 ccccgatgag gccgtgttcg gcgatggccg ggtacgtatc ggtcatatcc gtgctcctcc
     3421 gcgccggatc ggtggggcgg tgcccagtgc agtcgctcgg gcgctgatgc acatcacccg
     3481 atgagcatga ttcgccgccg ccgcacctgt cataccgtcg tccccggccc atgacaacgg
     3541 cggtccccgc cgtgaacagc cggaggtcag catgacgctc gaaggcaagg ttgcgatcgt
     3601 gacgggtggc aacagcggca ttgggaaagc cgtcacgctg gcgctcgccg agaagggcgc
     3661 aagcatcgtc atcgactacg tcgccaacga gaaggcgacc gaggagctcg agcaacagat
     3721 cgcgaagctc ggcgaccagt cgatcggcgt cgaagccgac gtgagcaagg tcgaagacct
     3781 cgagacgctc atcgagcagg cagtgaaggc attcgggcgt atcgacgtca tggtcaacaa
     3841 cgccggcatc gagacgcgct cgtcggtgct cgacacgtcc gaggaccagt acgaccgtgt
     3901 cctcgcgatc aacctgaaga gcgccttctt cggcacacag atcgcagcga agcagatgat
     3961 cgcccagggt ggcggcggca cgatcatcaa catcacatcg gtgcacgaag actggccgat
     4021 gcccggcaac accgcgtact gcctgtcgaa gggcggcatg cgcatgctca cccgcaccgc
     4081 cggcgtcgaa ttgggtgtgc acggcatccg cgtgatcggt gtcggtccgg gggcggtcgc
     4141 gacaccgatc aatacgcaga cgatgaacga cccggccgcg atgaagacgc tcgacaacgc
     4201 gatcccgctc gggcgtatgg cccagccgca cgagatcggc caggtcgtcg cgttcctcgc
     4261 gagcgacggc gcgtcgtaca tgaacgcgac cacggtgttc gccgacggcg ggctcatgca
     4321 cagcagcccc ggcctgtagg agcgcgccat gccagagcgt tacgacgtca tcatcatcgg
     4381 gaccggtgcg ggcggcggaa cgctcgccca cacgattgcg ccgtccggca agaaggtatt
     4441 gatcctcgaa cgcggcaact tcctgccgcg tgagatggag aactggaccc cggcgcccgt
     4501 cttcatcgac ggccgctaca tctccgaaga cacgtggtac gacgcgtcgg gcaaggcgtt
     4561 ccaaccgcag gtgcactatt tcgtcggcgg cgcgacgaag ttgtacggcg ccgcgttgta
     4621 ccggctgcgg ccgcaggact tcggtgaggt gaagcacgtc gacggcgtgt cgccggaatg
     4681 gccgctcacc tacgacgact tcgagcccta ctactccaag gccgaatggc tctaccaagt
     4741 gcacgtgaat cacggtgagg acccgaccga aggccatttg agcaagcagt atccgtggcc
     4801 ggcggtgtcg cacgagccgc gcatccaaca agtagccgac gacttggaga aagcgggcta
     4861 ccacccgttc cacgcgccgt gcggggtgct gctggacgag gcggaacgcg cgaagagcac
     4921 ctgcatccgg tgcacgtggt gtgacggcta cccgtgcctc gtgcacgcga agtcggacgc
     4981 ggagacgatc gcggtgcggc cgatcatcga cctaccgaac gtgacgttgc tcgtcgacac
     5041 cgaagtcgtg caactggcaa cgaacgcgtc cggccgtgcg gtgaccggtg tcgtcgtctc
     5101 acgcggcggc gaacgagagg tgtacgaagc cgacatcgtc gtgctgtcgg cgggcgcgtc
     5161 gaacagcgcc aagatcctgc tcgcgtcggc aggcgaccag catccgaacg ggctcgcgaa
     5221 cggttcggat caggtcggcc gcaactacat gttccacaac agcaaggcgg tcgtggcgct
     5281 gtcgaaggaa cgcaacgaca cgacgttcca gaagacactc ggcctcaacg acttctactt
     5341 cggtgccgac gactacacat ggccgatcgg caacatccag atggtcggca agtcgaacgc
     5401 ggaggcgatg aagggcgagg agccgaagct caccaagctt gcgccgcact ggagcctcga
     5461 cgacgtcgcg catcacgcgg tcgacttctg gctcacgacc gaagacctgc cgctcccgga
     5521 gaaccgggtg acgatcgacg gcgatcgcaa cgtgcacctc gcgtacaagt cgacgaacga
     5581 cgaggaggcg gggcggctct atcacgagct gaggaagatc ctgaaccatg tcgggatggc
     5641 cgaccaccac gtcctcgaca agaacttcta catggacatg agcatcccgg ttgcgggatg
     5701 tgcgcaccag gccgggacgt gtcgtttcgg ccgggacgcg tcgacctcgg tgctcgacga
     5761 gaactgccga gcacacgagg tcgacaacct ctacgtggtc gacaccagct tcttcccgag
     5821 catcggagcg gtgaaccccg cgctgaccgc gatggcgaat gccattcgcg tcggcgagca
     5881 cctgctcgaa cgcatgagct gacccgaacg ggacggaaga ccgagccgat ggcgacggag
     5941 acggaacaac gcgtcgttca gatcgcggcc gtcgcgcaag gcgtcgcact cgtcacattt
     6001 ccggccgcca gcacgatctt cacgaacgct gacgactacg acctgtcgcg gagcgcgtat
     6061 ggcgcgatgt tcgtcccgca ggccgtcacg gcggtggccg cgtcggtcct cggcgcgtcc
     6121 tgggccgctc gcgtcgggac gcggaagatc tacctgatcg gactcaccgc gaacgtcgtc
     6181 gcgatggcgc tgctggtggt cagtgcgacg gtgcagcacg acgagtcgat cgcgtacccg
     6241 atcctgttgc ttgcgaccgg gtgcctcggc gtcggcttcg gcttcaccgt gccggcgctc
     6301 aacacgttca ccgcggcgtt caatccagcc aaggtcgacg cgtccgtcct ggtgctcaat
     6361 gcgctgctcg gcgtcggcac tgcgctcgcg ccggtgctcg ttgcggtgtt cgtgggcctc
     6421 gatctctggt ggggccttcc ggcgctcacc ggaatcgtgc tcgtcggtct gctcgtcgcg
     6481 agctcccggt tggcgttcgc ggtcgattcg tcggcgagac cgcccgaggc gactgcgcgc
     6541 cggtcgatcc ctgtgcgctt ttggatctac gccgggttcg ccctgtgtta cgggatctgc
     6601 gagacgatga acgggaactg ggcgtcgctc gacatgaagg acctcggcgc gtcgacaaca
     6661 caggcatcgc tcgcgctcac gaccttctgg gcgtctgtca ccctcggacg cgtcgtgttc
     6721 gccgctctcc agcgctgggt gccgacgagc ctcatctacc gagcgttgcc cgtactgctc
     6781 gtcgcggtgt tcctcgcgat ctcacaactg ccggacgacg aacccgcgct cgcgatcgtc
     6841 acgttcgggc tcgcaggcct cgggtgttcc gcgttgctcc cgctcacgat cagcttcggg
     6901 cagggtgagc tgaccgcgat gagcgccggc gttgccgggg gtgtcatcgc gagctaccag
     6961 gtcggctacg ggatcgcggc ctttggcgcg gggccgttac agtccgcagg cgtcgacctg
     7021 gccgcactgt tcgcctggag cgcggtcgtc gccgccgtca tggccgcgct ggcgctcgtg
     7081 ctcacgcgtt cccgtgcgga cggtctcgcc gacctcggtg ctcgacgcga actgccgagc
     7141 acacgaggtc gacgaactct caactagccg acgatcttgg ccttcatctt ggcgaactcg
     7201 gcgtcgtcga tgacaccctt cgccttgagg tcggcgagac gggccaactc gtccgcggaa
     7261 ctgccgccgc ttccggccgc gtcgcggatg taggcgcgcg ccgcctcatc ggctttctgc
     7321 gcgtctgcca tcgcgtgctc cgacatcttg tgcccgcgcg cgatcaagta cacgaacaca
     7381 cccagatacg ggaggaagat gatgaagatc acccagagga acttggcgaa tccaccgaga
     7441 tcgtggctgc ggaagatgtc cgagaacacg atgatcaaca ggtagatcca aatgaagaag
     7501 agcgtgaacc aaatcatggt ccacagcact tcaccaactt gccattcacc cagcatttgc
     7561 gctcctgttc acgcagctag aggaagcgcc gtctgagggc tcgctcccgc gccatcaccg
     7621 tgatggcctt gatgtgggag agaaacgtag cccgtggccg gacacccgac gagtatcacg
     7681 aacaccgcaa acgctgattc acccttgaag gcgaccctta cacgtagtcg ttgcggcgga
     7741 gccattgcag cgagaaccag ccccagatgg ggggcaggta cgcggtgaac agccggtgcg
     7801 tgaacgtcgc ggcgaccgct tgcgcttcgg ggatcccggc cgcggtcagg cccgcgatca
     7861 tgcccgcctc gacgacgccc atgccgccgg gcaccggtgc catgccgccg agtagcgacg
     7921 ccaacgagtt gacgaccatg agctgcagca cggggaggtc ttggccgtac gcgtgcagcg
     7981 acgcgtcgag cacgagggcg tagaggatct gcgacgcgag gttgccgccg aagagcatga
     8041 cggctttgcg cggcgtcgag aggatgaggc gcaggttctc cttggcggcg tcccattgcg
     8101 gcgcgatcaa gcccttcagc cgccggcgca gcttcggcac gacgagcgcg agtgcgagcg
     8161 cgatgccgcc gacgatgatc accaggatca cgacgcccgc gatggtgcgg tcgccgccgg
     8221 tgctcgacgg gatgaactcg tcgccggtga ccgcgaggcc gatgatgatg acgaacgtct
     8281 gcacgacgaa gcccgcgaag gagttcaaga tgccggagct cgcggcgacc gcgatcttct
     8341 gcccttgctt ctggaagaac cggatgacga gcgcggtcgt cgcgaccgtg ccgccgacga
     8401 gaccggtgaa gttgttcgcg aactgttcgc cgagcacgcc tctgaa
//
LOCUS       DASUGA010000347         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2030423, whole genome shotgun sequence.
ACCESSION   DASUGA010000347 DASUGA010000000
VERSION     DASUGA010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2030423"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..>2050)
                     /locus_tag="VFX21_06970"
     CDS             complement(178..>2050)
                     /locus_tag="VFX21_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095733.1"
                     /translation="TFLDKVIPTCTATQAVTWNGTSFSCIALSAGSTPGGADKQVQFN
                     DAGTFGTDAAITWTKNTDQLSIDTTAPPAVGADRTLYRANHNFNYNNDGVVIGYLSTM
                     ALAQIVSGAPTANVTGFRAEGFFTNSSTSSPAIGSHVGGFVSAQTSLSVTGVSAAHPI
                     YAYDDISIMRQQAGSPPQPGQSAISFFSRPQIQATGTANLQYFATIGLNSQYTYSVDP
                     GASITVPRRVGVWFSPATKVGSGTLNLGEEAAVFVGANSFLGTSAYSLHSLNSSVMID
                     NAGPMFIREKVSFEDQNVSTSGATPTGGTIDPALEFWAGGAPNSPTAIGIDAPFSFDF
                     LSSALLYVHSNTTRTGSLGEFLFAKWDGLHDYDTGATFFGADYYFFYGIPTFKIDETR
                     TIPGSGGIVYKPTFRPTGAGTVMTLDSASLPYTADIFAGSVFSSSGGGTYGAGSYRVG
                     LRLQPEVKTGVTMGGTYTSAEILGKYAGTGTLGLHVGLYVAGLVSTSIPRGIWNASTT
                     MLECASTDVASTTKVFDPRSTCIEFSPTAGCCAVPPCDCTQTATPTINDGVVGQILIL
                     RSKDVSGRTVTLQDQGTLAGTNIEMSTGTFVMGNGDTLVLQWSDAMANWYEVARSNN"
BASE COUNT          360 a          625 c          693 g          372 t
ORIGIN      
        1 cgaggaagcc ccactgcttc gtggtcaggc tcgatcccgt tcgcgcgttg tacgcgtccc
       61 gcgccgccgt cagcgacgcg agcttcgcgt ccggaatcgt gaccgtcacg tccgccgcgt
      121 cgcttctttt gcttaggacg acgatgctta atgcgagaca tagcacgacc ctcttcatca
      181 gttgttgctc cttgcgacct cgtaccagtt agccatggcg tcgctccatt gcagaacgag
      241 cgtgtcgccg ttccccatca cgaaggttcc cgtcgacatc tcgatgttcg taccagcgag
      301 tgtgccctgg tcctgcaggg tcactgtacg ccccgagacg tctttgctcc ggaggatcag
      361 gatttgcccg acgacgccgt cgttgatcgt cggcgtcgcc gtctgtgtgc agtcgcatgg
      421 cggtacggca cagcagcccg cggtgggaga gaattcgatg caggtcgagc gcggatcgaa
      481 gaccttcgtc gtcgaagcga cgtccgtcga ggcgcactcg agcatcgtcg tcgaggcatt
      541 ccaaatgccg cgcggaatcg aggtcgaaac caggcccgcg acatagagac cgacgtgcag
      601 tccgagcgtg cccgtgccgg cgtacttccc gaggatctcg gcagaggtgt aggtgccgcc
      661 catggtgacg ccggtcttca cctcgggctg aaggcgaagg ccgacgcgat aggagcccgc
      721 accgtaggtg ccgccgcccg agctcgagaa caccgagccg gcgaagatgt ccgcggtgta
      781 cggaagcgac gcactatcca gcgtcatgac cgtgccggcg ccggtcggcc ggaaggtcgg
      841 cttgtagacg atgcccccag agcccggaat cgtgcgcgtc tcatcgatct tgaaggtcgg
      901 aatgccgtag aagaagtagt agtccgcacc gaagaacgtc gcgccggtgt cgtagtcgtg
      961 cagaccgtcc cacttcgcaa acaagaattc gccgagcgac ccggtgcgag tcgtgttgga
     1021 gtgtacgtag aggagagctg agctgaggaa gtcaaacgag aacggcgcat cgataccgat
     1081 cgccgtcggg ctattcggcg caccgccggc ccagaactcg agcgccggat cgatcgttcc
     1141 gccggtcggc gtcgcgcccg acgtcgagac gttctgatcc tcgaaggaga ccttctcgcg
     1201 gatgaacatc ggcccggcgt tgtcgatcat gacgctgctg ttcaagctgt gcagcgagta
     1261 ggcgctcgtg ccgaggaagg aattcgcgcc gacgaagacg gccgcctcct ccccgagatt
     1321 cagcgttccg ctgccgacct tcgtcgccgg cgaaaaccag acaccgacac ggcgcgggac
     1381 cgtgatcgag gcgccggggt cgactgagta ggtgtactgc gagttgagac cgatcgtggc
     1441 gaaatactgc aggttcgcgg tgccggttgc ctggatctgc gggcgcgaaa agaaagagat
     1501 cgccgactgt cccggttgcg gtggcgagcc tgcctgctgg cgcatgatcg agatgtcgtc
     1561 gtaggcgtag atcggatggg cggcgctcac gcccgtcacg ctgagcgagg tctgcgccga
     1621 gacgaagccg ccgacgtggc tgccgatcgc tggcgagctc gtcgaggagt tggtgaagaa
     1681 gccctcggcg cgaaagccgg tcacgttcgc ggtcggcgcg ccgctcacga tctgtgcgag
     1741 cgccatcgtc gagaggtagc cgatgacgac gccgtcgttg ttgtagttga aattgtgatt
     1801 ggcgcggtag agcgtgcggt cggcgccaac cgccggcggt gccgtcgtat cgatcgagag
     1861 ctggtcggtg ttcttcgtcc aggtgatcgc cgcgtcggtg ccgaacgtgc cggcgtcgtt
     1921 gaactgcacc tgcttgtcgg cgccgccggg cgtcgaaccg gccgagagtg caatgcagga
     1981 gaagctcgtg ccgttccagg tgaccgcctg cgtcgcagta caggtcggga tcaccttgtc
     2041 gagaaacgtc
//
LOCUS       DASUGA010000348         2695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_294755, whole genome shotgun sequence.
ACCESSION   DASUGA010000348 DASUGA010000000
VERSION     DASUGA010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294755"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VFX21_06975"
     CDS             <1..891
                     /locus_tag="VFX21_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095734.1"
                     /translation="TCNGHAELCERAYDEVVQVATHNSMSSPDVVQIWPEQDGTIREQ
                     LDAGVRALLIDTHYWPGVTAPDQLTGLEPTVPANVANRVFETGGDRLRPHDGVFLCHN
                     NCALGGEPFADGLAQLRDFLVANPDEVVTLVVQDETSSADTTAEITKAGLEPFLYDHA
                     AHDDWPTFGELIDAGERLIVFAENEGPPPSWYRNAFDDMQDTPFGFPTPDAMTCEHNR
                     GADDATLLLMNHWVSRVAPDRATAAVVNAHDFVVDRARECEKARGRLPNFVAVDFYSI
                     GDVVGAVDTLNGFGSGSEDT"
     gene            888..2573
                     /locus_tag="VFX21_06980"
     CDS             888..2573
                     /locus_tag="VFX21_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009477343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX5095735.1"
                     /translation="MSVPLFTSLHGYDRKWFRGDLIAGLTVWAVLVPEALAYASIAGV
                     SPVVGLYAAPPALILYAMFGSSKHLVTGPMAATAALSAAAVADLVTDTRDFPAFTAAV
                     ALMTGAIALLAGLLRLGFLANFISEPVLKGFIIGLALTIMIGQVPKLFGVEAADGDFF
                     RQLWEFLGNVGDAHGRTTLVGLGSLALVLILRKIAPAIPGSLVAVLLGIALVHLLDLD
                     DKGVAIVGHIDSGLPSFGAPDAIGIHEYFATAAASVGIMLVGFAEGLGAAKTYATRNH
                     YDVDANRELIGLGAANIGAGFSSGMVVNGSLSKTAVNGGAGAQSQLSGLFVAALTVVT
                     LLFLTGLFEDLPEATLAAVVIAALIELVDFPALAELYRLYTRRLGQIYGPAARPDFIA
                     AVAAMFGVLLFDTLPGLFIGIAVSLLLLLYRASKPRVSVLGKVPGANETYADRERHPE
                     YTETPGIATLRVESGLFFANADAVRDAIRTHAADTSTRAIVLDAESIAFIDVTAVRML
                     RELAEDLATRKIQLVLAHEIGQVRDVMRRESGEESALAVYPTIQAAVDALGAQ"
BASE COUNT          411 a          920 c          914 g          450 t
ORIGIN      
        1 acctgcaacg gtcacgccga gctgtgcgag cgtgcatacg acgaggtcgt gcaagtcgcg
       61 acccacaact ccatgtcgag ccccgacgtc gtgcagatct ggcccgaaca agacggcacc
      121 atccgcgagc agctcgacgc gggcgtgcgg gcgttgttga tcgacacgca ttactggccc
      181 ggtgtcacgg cgcccgacca gctcacgggg ctcgagccga cggtgccggc caatgtcgcg
      241 aaccgagtgt tcgagaccgg cggcgaccgg ctgcggccgc acgacggcgt gttcctgtgc
      301 cacaacaact gcgcgctggg cggcgagccg ttcgccgacg gcctcgcgca actgcgcgac
      361 ttcctcgtcg ccaatcccga cgaggtggtc acgctcgtcg tccaggacga gacgtcgtcc
      421 gcggacacga ccgcggagat caccaaagcc gggctcgagc cgttcctcta cgaccacgcc
      481 gcccacgacg actggccgac gttcggcgag ctgatcgacg cgggcgaacg actgatcgtg
      541 ttcgccgaga acgaaggtcc gccgccgtcg tggtatcgca acgccttcga cgacatgcag
      601 gacacgccgt tcggattccc gacgcccgac gcgatgacgt gcgagcacaa ccgaggtgcc
      661 gacgacgcca cgctgctgct gatgaaccac tgggtctcgc gggtcgcgcc cgatcgagcg
      721 acggcggcgg tcgtgaacgc gcacgacttc gtcgtcgacc gcgcgcggga atgcgagaag
      781 gcgcgcggcc ggctcccgaa cttcgtggcg gtcgacttct acagcatcgg cgacgtggtc
      841 ggcgcggtcg acaccctcaa cgggttcggg tcaggatcag aggacacatg agtgttcccc
      901 tgttcacgtc gctccacggc tacgaccgga agtggttccg cggtgacctg atcgcaggcc
      961 tgaccgtctg ggccgttctc gttcccgagg cgctcgcgta cgcgtcgatc gcgggcgtct
     1021 cccccgttgt cggtctctat gccgcgccgc ccgccctgat cctctacgcg atgttcggca
     1081 gctcgaagca cttggtgacg ggaccgatgg ccgcgaccgc ggcgctgtcg gccgccgcgg
     1141 tcgccgacct cgtcaccgat acgagggatt tccccgcgtt cacggcggcg gtcgccctga
     1201 tgacgggtgc gatcgcgctg ctcgccggac tgttacggct cgggttcctc gcgaacttca
     1261 tctccgaacc ggtattgaag ggcttcatca tcgggctcgc gctcaccatc atgatcgggc
     1321 aagtaccgaa gctgttcggc gtcgaagcgg cagacggtga cttcttccgc cagttgtggg
     1381 agttcctcgg caacgtcggt gacgcgcacg gccgcacgac gctggtggga ctgggatcgc
     1441 tcgcgctcgt cctcatcttg cgcaagatcg cgcccgcgat acccgggtcg ttggtcgcgg
     1501 tgctgctcgg catcgcgttg gtgcacctgc tcgacctcga cgacaaaggc gtcgcgatcg
     1561 tcggccacat cgacagcggg cttccgtcct tcggggcgcc ggacgcgatc ggcatccacg
     1621 agtacttcgc gaccgcggcc gcgtcggtcg gcatcatgct ggtcgggttc gccgaaggac
     1681 tcggtgccgc gaagacctac gcgacacgca accactacga cgtcgacgcg aaccgcgaac
     1741 tgatcggcct cggcgccgcg aacatcggcg ccgggttctc gagcggcatg gtcgtgaacg
     1801 ggagcctgtc caagaccgcg gtcaacggcg gtgccggcgc gcagtcgcag ctctcggggc
     1861 tcttcgtcgc ggccctgacc gtcgtgacgt tgctcttcct gaccggcctc ttcgaggacc
     1921 tcccggaagc aacgctcgcc gcggtcgtga tcgcagcgtt gatcgagctg gtcgacttcc
     1981 ccgcgctcgc cgagctgtac cgcctgtaca cgcgccggct gggccagatc tacgggccgg
     2041 ccgcccgccc cgacttcatt gcggcggtcg cggcgatgtt cggcgtgttg ctcttcgaca
     2101 cgctgccggg gctgttcatc gggattgccg tctcactgct gctgttgctg taccgcgcgt
     2161 cgaaaccgcg ggtcagcgtc ctgggcaagg taccgggcgc gaacgagacg tacgcggacc
     2221 gcgagcggca tccggagtac accgagactc cgggcatcgc gaccctgcgt gtcgagagcg
     2281 gcctgttctt cgccaatgcg gatgccgtcc gcgacgcgat ccggacgcat gcggctgaca
     2341 cgtcgacacg cgcgatcgtg ctcgacgccg agtcgatcgc gttcatcgac gtgacggcgg
     2401 tgcgcatgtt gcgcgagctg gccgaggatc tcgcgacccg gaagatccag ctcgtgctcg
     2461 cgcacgagat cggtcaggtg cgcgacgtga tgcggcggga gtcgggcgag gaatccgcgc
     2521 tggccgtgta cccgacgatc caggccgcag tcgacgcgct cggtgctcag tagacctcgg
     2581 gaacgaacgt ctgctcgtcg acgggcgggc gcacgtagtt gtcgggcccg cgcgacggca
     2641 tcgtcagcgg cgccgaggtg acgtcctcgt acggaatctg cgacaggaga tggct
//
LOCUS       DASUGA010000349         3314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1142111, whole genome shotgun sequence.
ACCESSION   DASUGA010000349 DASUGA010000000
VERSION     DASUGA010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142111"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1526
                     /locus_tag="VFX21_06985"
     CDS             <1..1526
                     /locus_tag="VFX21_06985"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional glutamine-synthetase
                     adenylyltransferase/deadenyltransferase"
                     /protein_id="HEX5095736.1"
                     /translation="QMHLAVVRSIHEKLYFAPLLDTLAGAGPLSPAAVEERLAAFGFT
                     DVEQTRAALHELTAGLTRRSRVMQQLLPVILGWLSSTPDPDLGLLQLRRLTEGYTRSS
                     TLARRFRETPIAAERTSRLLGSSRVLGLAMHRHPDFVDALANDAILAVEPARAEIVAE
                     AVESLDWREDDEARLAGLRRFKRRHLLRIGARDLLGFADLAGVGRELSNLGDATVAAA
                     LDSLEPSIPFAVIGMGRLGGGELSYASDIDVLFVSETSGAREFEAAERVATRLLRAIG
                     DPTAEGQTFRIDARLRPEGAQGVLTRSLDGYRGYWEQWAQPWEFQALTKARYVAGDEE
                     LAGRFLETAHRYVYRDPFPSEWRREIRRMKARIERERIPPGEDPQFHLKLGRGSLSDV
                     EFTVQLLQLESGHREPSVRATSTLGALHALVAVAAISAVDAAHLADAYVLCERARNYR
                     YLLTGAPGDALPIGDDAAKLGRMLGYGREPQQSLREDYRRLTRRSRDVVERIFYGRE"
     gene            1547..2107
                     /locus_tag="VFX21_06990"
     CDS             1547..2107
                     /locus_tag="VFX21_06990"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016471352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5095737.1"
                     /translation="MPERWDGGHYQRKFDELAASGSNVHGEVDFLMTLEPATVLDAGC
                     GTGRVAIELARRGIDVVGVDAEDDMLATARERAPQIEWVTSDLTELDLGRTFDVVVMA
                     GNVPLFTEPARRDALAAGCARHVAPGGALVAGFSNELRGRTYSFGEYDAHCEAAGLVL
                     DTRYATWDRDPFDNGNYAVSVHRHAR"
     gene            complement(2157..2417)
                     /locus_tag="VFX21_06995"
     CDS             complement(2157..2417)
                     /locus_tag="VFX21_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD family protein"
                     /protein_id="HEX5095738.1"
                     /translation="MKVRIPTPLRSYTAQQAVVEADGATVSAVLEDLDARYPGLRFRM
                     VDEQNQIRKHMKVFVNDESVRDLATPLADGDEVTIMQALSGG"
     gene            complement(2414..>3314)
                     /locus_tag="VFX21_07000"
     CDS             complement(2414..>3314)
                     /locus_tag="VFX21_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX5095739.1"
                     /translation="WKEASRPPAFPTGDRLGRAVKRVFWLTPGHASEPGVWYAGGTPQ
                     SLFRTEDNGDTWESFAGWNDHPMYETWCEFPEEQTPDGSMLHSIIVDPRDAKHLYLGL
                     SGGGIFESTDGGADWAPLNGGVAMDFAPDDTAEFGHDPHCVRMHPRQPDRLYHQNHCG
                     IYRIDRPEKQWVRIGDNMPRDVGDIGFPIELHPRDPDTAWVFPMDGTDVWPRTSPDGR
                     PAAFMTRDAGASWTRCDNGLPERAWYTVKRQAMTVDDRDPVGVYFGTTSGEVWASSDE
                     GARWTCIAQHLPEIYSVEFADFA"
BASE COUNT          467 a         1148 c         1166 g          533 t
ORIGIN      
        1 atcagatgca cctcgcggtc gtgcgttcga tccacgagaa gctgtacttc gcgccgctgc
       61 tcgacacgct tgcgggcgcg gggccgctgt cgcccgccgc ggtcgaggaa cggctggccg
      121 cgttcggctt caccgacgta gagcagacac gcgccgcgct gcacgagctg accgcgggcc
      181 tcacccgccg gtcgcgcgtc atgcaacagc tcttgcccgt catcctcggc tggctgtcgt
      241 cgacacccga tcccgacctc ggcctgttgc agttgcgccg cctcaccgag ggctacacac
      301 gttcgtcgac gctggcgcgc cgattccggg agacaccgat cgcggcggag cgcacgtcgc
      361 ggttgttggg ttcgagtcgt gtgctcggcc tggcgatgca ccggcatccc gacttcgtgg
      421 acgcgctcgc caacgacgcg atcctcgcgg tcgagccggc gcgggccgag atcgtcgcgg
      481 aggcggtcga gagcctcgac tggcgcgagg acgacgaagc gcggctggcc ggactgcgcc
      541 gtttcaagcg ccgccatctg ttgcgcatcg gcgcgcgcga cctgctcggg ttcgccgatc
      601 tcgccggcgt cggacgcgag ttgtcgaatc tcggcgatgc gacggtcgcg gccgcgctcg
      661 actcgctcga gccctcgatt cccttcgcgg tcatcggcat gggccggctc ggcggcggtg
      721 agttgtcgta cgcatccgac atcgacgtgt tgttcgtgtc cgagacgtcg ggcgcgcgtg
      781 agttcgaggc ggcggagcgc gtcgcgaccc ggctgctgcg cgcgatcggc gaccccactg
      841 ccgaggggca gacgttccgg atcgacgcgc ggttgcggcc cgaaggcgcg cagggcgtgc
      901 tcacgcgttc gctcgatggt taccgcggct attgggagca atgggcgcag ccgtgggagt
      961 tccaggcgct gacgaaggcg cgctacgtgg cgggcgacga ggagctcgcg gggcgattcc
     1021 tcgagacggc gcatcgatac gtctatcgcg atccgttccc ctccgagtgg cggcgcgaga
     1081 tccggcgcat gaaggcgcgc atcgaacgcg aacgcatacc gccgggcgaa gacccgcagt
     1141 tccacctgaa gctcggacgc gggtcactgt ccgatgtgga gttcacggtg cagctcctgc
     1201 agctggaatc gggacaccgg gaaccgtcgg tgcgcgcgac ctcgacactc ggcgcactgc
     1261 atgcactcgt cgccgtcgcg gcgatcagtg cggtggacgc cgcgcatctc gccgacgcgt
     1321 acgtcctgtg cgagcgcgct cgcaactatc gctacctgct cacgggcgca cccggtgacg
     1381 cgctgccgat cggcgacgac gcggccaagc tcgggcgcat gctcggctac ggccgcgagc
     1441 cgcagcaatc gctgcgcgag gactaccggc gcctgacgcg acggtcacga gacgtcgtcg
     1501 agaggatctt ctacggccgc gagtagcccg ccactatcgt cggcccatgc ccgagcgctg
     1561 ggacggcggc cactaccagc ggaagttcga cgagctcgcg gcgagcggtt cgaacgtcca
     1621 cggcgaggtg gatttcctca tgacgctgga gccggcgacc gtgctcgacg ccgggtgcgg
     1681 caccgggcgg gtcgcgatcg agctcgcgcg gcgcggcatc gacgtcgtcg gtgtcgacgc
     1741 cgaggacgac atgctggcaa cggcgcgcga acgggcgccg cagatcgagt gggtcacgag
     1801 tgatctgacc gagctggatc tcggtcgcac gttcgacgtg gtcgtgatgg ccggcaacgt
     1861 gccgctgttc accgagccgg cgcgccgcga tgcgttggcg gccggttgcg cgcgtcatgt
     1921 cgcgccgggt ggcgcgctgg tcgccggctt ctcgaacgaa ctgcgcggcc gcacgtattc
     1981 cttcggcgag tacgacgcgc actgcgaagc cgcgggactc gtgctcgata cgcgttacgc
     2041 gacctgggac cgcgacccct tcgacaacgg caactacgca gtctccgtgc accgccacgc
     2101 gaggtagccg ccgagcaacg agcggcccgg tcgatcgccg tgcgcccgga cgtggatcaa
     2161 ccgcccgaga gggcctgcat gatcgtcacc tcgtctccgt cggcgagggg cgtcgcgagg
     2221 tcgcgcaccg actcgtcgtt cacgaagacc ttcatgtgct tgcggatctg gttctgctcg
     2281 tcgaccatgc ggaaccgcag gcccgggtag cgcgcgtcga gatcctcgag cactgcactg
     2341 acggtcgcgc cgtcggcttc cacgaccgcc tgctgcgcgg tgtacgaccg caacggtgtg
     2401 gggatgcgga ccttcatgcg aagtcggcga actccaccga gtagatctcg ggcaggtgct
     2461 gcgcgatgca cgtccagcgc gcgccttcgt cgctgctcgc ccagacttcg ccgctcgtcg
     2521 tcccgaagta cacaccgacc gggtcgcggt cgtcgaccgt catcgcctgg cgcttcaccg
     2581 tgtaccacgc gcgttcgggc aggccgttgt cgcagcgcgt ccacgacgca cccgcgtcgc
     2641 gtgtcatgaa cgcggcgggt cggccgtcgg gactggtgcg cggccacacg tcggtgccgt
     2701 ccatcgggaa cacccacgcc gtgtcgggat cacgcggatg caactcgatc gggaaaccga
     2761 tgtcgcccac gtcgcgcggc atgttgtcgc cgatgcgcac ccactgcttc tcgggccggt
     2821 cgatgcggta gatgccgcag tggttctggt ggtagagccg gtcgggctgc cgcggatgca
     2881 tgcgcacgca gtgcgggtcg tgaccgaact cggcggtgtc gtcgggcgcg aagtccatcg
     2941 cgacgccgcc gttcaacggt gcccagtccg cgccgccgtc ggtgctctcg aagatcccgc
     3001 cacccgacag gccgaggtag aggtgcttcg cgtcgcgcgg gtcgacgatg atcgagtgca
     3061 acatcgagcc gtccggcgtc tgctcctccg ggaactcgca ccacgtctcg tacatcgggt
     3121 ggtcgttcca gccggcgaag ctctcccatg tgtcgccgtt gtcctcggtg cggaacaggc
     3181 tctgcggcgt cccgcccgcg taccaaacgc ccggctcgct cgcgtgtccc ggcgtcagcc
     3241 agaacacccg cttgaccgcg cggccgagac ggtcaccggt cgggaacgcg ggcgggcggc
     3301 tcgcttcctt ccac
//
LOCUS       DASUGA010000350         2738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1947395, whole genome shotgun sequence.
ACCESSION   DASUGA010000350 DASUGA010000000
VERSION     DASUGA010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1947395"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..538)
                     /locus_tag="VFX21_07005"
     CDS             complement(20..538)
                     /locus_tag="VFX21_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX5095740.1"
                     /translation="MPCYALGDAEPQIDPDAFVHPEATIIGNVVIGPGSTIWPQAVLR
                     GDQSRIVIGARTSVQDGAVLHCTRELETVVGDDCTIGHLAHLEGCTVEDGALVGTGSI
                     VLHAAIVRSGALVGAAALVPGGMEVPPGAMALGVPAKIKPDAVKPAQITYPASEYQRN
                     WARYRAEMRRID"
     gene            558..1358
                     /locus_tag="VFX21_07010"
     CDS             558..1358
                     /locus_tag="VFX21_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX5095741.1"
                     /translation="MDVALHVKEVAENGYTIVENAIEPELVDELHDDLLRLEQFFDVQ
                     PSPNSFEGHHTLRVYNLLAYGKLYEKIPVHASVLPVVEGVLDPGCLISSLSSIAILPG
                     ETAQPIHADDQLIPLPKPHPPTVCNSMWALTDFTEANGATRIIPGTHLSDHSPDYGQP
                     YESIAAEMPKGSVLIWHGSLWHGGGANTTGERRVGIAMNYCAGYIRQQENQQLGLSRE
                     TVAGFEPRLRQLVGYSVYNMLIGHINKHSPDELLITGHDDHSMVWDGI"
     gene            1364..2155
                     /locus_tag="VFX21_07015"
     CDS             1364..2155
                     /locus_tag="VFX21_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5095742.1"
                     /translation="MRNLIVEDTDGVRVLTLNRPDKLNAFDTALYDACAAAIKEAEGR
                     DDIACVVLTGTGRAFSAGQDLGEMAAIDTSAPSAEQSAGHGFPRFIDTVAAFEKPFIV
                     AVNGLGVGIGMTVLLHCDIALIARGARLRAPFVPLGVVPEAAGSLLMPIVMGNQRAAE
                     SLYTGDWITAEEAVAGGLALRVVPGETLMDETMQLARRIAAGPVRSLVETKKLVLAGR
                     IDAVRAARAREDAVFARMVGAAANADALTAFLDKGQPPPDPTRRP"
     gene            2193..>2738
                     /locus_tag="VFX21_07020"
     CDS             2193..>2738
                     /locus_tag="VFX21_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEX5095743.1"
                     /translation="MAIVDTRSGKVEGLEHHGICEFRGIPYAAPPVGPLRWLAPRREA
                     AWDGVRDATKFSAICAQGDFAMETLMGGTAPVKSEDGLYLNVYTPGCDDARRPVMVWI
                     HGGAFLFGHGSTPWYDGTRFAVNGDVVIVTINYRLAAFGYLHLADLFGPEFEGSGNAG
                     VLDQAAALEWVRDCIANFGGDP"
BASE COUNT          447 a          967 c          902 g          422 t
ORIGIN      
        1 cgagcaccat cgccgcgcgc taatcaatcc ttctcatctc ggcgcggtag cgggcccagt
       61 tccgctggta ctcgctcgcc gggtacgtga tctgcgcggg cttcaccgcg tcgggcttga
      121 tcttcgcggg gacgccgagc gccatcgcac ccggcgggac ttccatccca cccgggacga
      181 gtgccgccgc gcccaccagt gcgccgctgc ggacgatggc cgcgtgcaac acgatcgagc
      241 cggtgccgac gagggcgccg tcctcgacag tgcatccttc gagatgggcg aggtgcccga
      301 tcgtgcagtc gtcgccgacg acggtttcca gctcgcgcgt gcagtgcagc accgcgccgt
      361 cctggaccga ggtgcgggcg ccgatcacga tgcggctctg gtcgccccgc aacaccgctt
      421 gcggccagat cgtcgagccg ggaccgatga cgacgttgcc gatgatcgta gcttcgggat
      481 gcacgaaggc gtccggatcg atctgcggtt cggcgtcacc gagggcgtag caaggcatgc
      541 gcggtagttt gacgtgcatg gacgtcgccc tgcacgtcaa ggaagtcgcc gagaacggct
      601 acacgatcgt ggagaacgcg atcgagccgg agctcgtcga cgaattgcac gacgacctgc
      661 tgcgcctcga gcagttcttc gacgtgcagc cctctccgaa ctccttcgag ggtcaccaca
      721 cactgcgggt ctacaacctg ctggcctacg ggaagctcta cgagaagatt cccgtgcacg
      781 cgagcgtgct gcccgtcgtc gagggcgtgc tcgatccggg ttgtctcatc tcgtcgctgt
      841 cgtcgatcgc gatcctgccc ggcgagaccg cgcagccaat ccatgccgac gaccagctca
      901 tcccgctccc gaagccgcat ccgccgaccg tgtgcaactc gatgtgggca ctcaccgact
      961 tcacggaggc gaacggcgca acgcgcatca tccccggtac gcacctgtcc gaccactccc
     1021 ccgactacgg ccagccgtac gagtcgatcg cggcggagat gccgaagggc tcggtgctga
     1081 tctggcacgg gagcctgtgg cacggcggtg gtgcgaacac gaccggcgag cggcgcgtcg
     1141 gcatcgcgat gaactactgc gcgggctaca tccgccaaca ggagaaccaa cagctcggct
     1201 tgtcgcgcga gacggtcgcg ggcttcgagc cgcgcctgcg ccagctcgtc ggctacagcg
     1261 tctacaacat gctcatcggc cacatcaaca agcactcgcc cgacgagctg ttgatcaccg
     1321 gccacgacga ccactcgatg gtctgggacg gcatctaaca ctcgtgcgca atctcatcgt
     1381 cgaagacacg gacggggtac gcgtcctcac gctcaaccgg cccgacaagc tcaatgcgtt
     1441 cgacaccgcg ttgtacgacg cgtgcgcggc cgcgatcaaa gaggcggaag ggcgcgacga
     1501 catcgcgtgc gtcgtcctca ccggcaccgg gcgcgccttc tccgcgggac aggacctcgg
     1561 cgagatggcc gcgatcgata cgtccgcgcc gtccgcggag cagtcggcgg gccacgggtt
     1621 cccgcgcttc atcgacaccg tcgccgcgtt cgagaagccg ttcatcgtcg cggtcaacgg
     1681 gctcggtgtc ggcatcggca tgaccgtgct gttgcactgc gacatcgcgc tcatcgctcg
     1741 cggtgcgcga ctgcgggcgc cgttcgtgcc gctgggcgtc gtgcccgaag ccgccggcag
     1801 cctgctgatg ccgatcgtga tgggcaacca gcgcgcggcc gagagcctct atacgggcga
     1861 ctggatcacg gccgaagaag cagtcgccgg cgggctcgcc ctgcgcgtgg tgccgggcga
     1921 gaccctcatg gacgagacga tgcagctcgc ccgccgcatc gccgcgggac cggtccggtc
     1981 cttggtcgag acgaagaagc tcgtgctcgc gggccgcatc gacgcggtgc gcgcggcccg
     2041 ggcccgcgag gacgccgtgt ttgcccgcat ggtcggcgcc gccgccaacg ccgacgccct
     2101 caccgccttt ctcgacaaag gacaaccacc ccccgacccc acccgccgcc cttagaagac
     2161 gggcttcgcc cggcggatct agggttccgc ccatggcgat cgttgacact cggagcggca
     2221 aggtcgaagg cctcgaacac cacgggatct gcgagttccg cggcatcccc tacgccgcac
     2281 cacccgtcgg accattgcgg tggctggcgc cgcgccgaga agcggcatgg gacggcgtgc
     2341 gcgacgcgac gaagttctcc gcgatctgcg cgcagggcga cttcgcgatg gagacgctca
     2401 tgggtggcac ggcacccgtc aagagcgaag acgggctgta cctgaacgtg tacacacccg
     2461 gctgcgacga tgcccgccgg ccggtgatgg tgtggatcca cggcggcgcg ttcctcttcg
     2521 ggcacggcag cacaccgtgg tacgacggca cgcgtttcgc ggtcaacggc gacgtcgtca
     2581 tcgtcacgat caactaccgc ctcgccgcgt tcggctactt gcacctcgcc gacctcttcg
     2641 ggcccgagtt cgaaggctcg ggcaatgcgg gcgtgctcga ccaggccgcc gcactcgagt
     2701 gggtacgcga ctgcatcgcg aacttcggcg gcgacccc
//
LOCUS       DASUGA010000351         3852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_11913, whole genome shotgun sequence.
ACCESSION   DASUGA010000351 DASUGA010000000
VERSION     DASUGA010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11913"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..989
                     /locus_tag="VFX21_07025"
     CDS             174..989
                     /locus_tag="VFX21_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX5095744.1"
                     /translation="MDALTFYGLEPTDDPCKWRMPIVPGLTSGRRALYGGAGLGAAIE
                     VMERVTGRPCVWATAQFLTYATPPSVLELDALEVVRGHQISQARVLAHVGDTEVVTVV
                     GAFGYRPLPFEGQWAQRPIVPPPDASPPRPLRNHHEGTISTRLDARLAAGRPFWEFPA
                     EQGSGTAALWVRMPELLEMSAASLAIIGDFVPMGIGQALGERAGGNSLDNTLRVAHRV
                     PTEWVLADIRVHAVADGFGHGLVHLWAEDGTLLGTASQSTIVRVWREEPEETS"
     gene            986..1942
                     /locus_tag="VFX21_07030"
     CDS             986..1942
                     /locus_tag="VFX21_07030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010595643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX5095745.1"
                     /translation="MTVPQRYGITFPFDGLPLLEQREIVRQLVDLGYTDLWSAESGGY
                     DAFIPLAVAAQWAPELRLGTAIVPAYTRGAHTLASTVASMCQAAPGQFALGLGTSSNV
                     IVERWNGIAFDKPYQRVRDTIRFLRKALTGEKVTEEYETFAVNGFRLGISVPEQPPIL
                     VAALREGMLRLAGREGDGAIINWLSADDVKTVVPHVGPDKEIAARIFVLPVDDAGMAR
                     AVGKRAIASYLTVPVYAAFHEWLGRGDELKDLWRLWKEGDRKAAADSIPDHVVDQLLV
                     WGTPAQCREHIQRYIDNGVTTPAPALFVGPEQLPGIITALKR"
     gene            1952..2410
                     /locus_tag="VFX21_07035"
     CDS             1952..2410
                     /locus_tag="VFX21_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020106995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEX5095746.1"
                     /translation="MFSVEDFLWYVDDAIDGMVAIVTELGDDLANTAIDTPGANSPYV
                     VLTHCLGVMEWWGGYFIAGRAIDRDRDAEFVARGSVADLARRSRAARDQLRADLVAFD
                     PHAPPAGTPAPEWADEPCARTQGGTALHIYRELAQHRGQMEITRDVLLTR"
     gene            complement(2400..>3852)
                     /locus_tag="VFX21_07040"
     CDS             complement(2400..>3852)
                     /locus_tag="VFX21_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX5095747.1"
                     /translation="SKVFGDLVPEAIARRHQVIPIGRRRGSILLAMADPDDVMVLDDL
                     RMRLGVPMIAVMADAAQVAAAIEEAYSRSAVRENLAAVATDDEFAEEEDLDDVEQGPI
                     VRLVDSLLEEAASDRASDIHIEPGRDGVKMRFRIDGVLHDISEASTSVLRPILSRVKV
                     LAELDIAQSRLPQDGRFTQRIGGDDVDVRVATVPTVYGEAAVLRLLDRSTGLVELSRL
                     GIAPEQLARYKTAFEAAQGAVLVTGPTGSGKTSTLYSTVAALDTRDRSVMSVEDPVEY
                     KLRGVKQMQVNRRAGLTFPVALRAMLRSDPDIILVGEIRDGETATIAAGAAMSGHLVF
                     STMHTTSAASSPARFIEMGVEPYVVTSAISCIVAQRLARRLCDACAAPLPDPEAILRR
                     MGVPEEAWETAKLRRAVGCDECHGTGFYGRVGCYEIMLMSDDIRELVLSRAPAQDIER
                     YAIAEGMQTLRDSGMSHVLAGQTTVEEMLRVIS"
BASE COUNT          606 a         1367 c         1294 g          585 t
ORIGIN      
        1 acacgggcgt cgctcgccca caattattgg ggtctctcga cgcccgacct agtctcccgg
       61 catcacgatc tgacctcaca cggcgccggc gagcgaagcg agcctggccg gaacggaagg
      121 cttgccggca acttgactcc aacacgcgga caagcctgaa gcgacaaaga gaagtggatg
      181 cgctgacctt ctacgggctg gagcccactg acgatccgtg caagtggcgg atgccgatcg
      241 tgccggggct cacgtcgggg cgacgcgcgt tgtacggcgg cgcggggctc ggcgccgcga
      301 tcgaggtgat ggaacgggtc accgggcggc cgtgcgtgtg ggcaactgcg cagttcctca
      361 cgtacgcgac gccgccgtcc gtgttggagc tggacgcgct cgaagtcgtg cgcgggcatc
      421 agatctccca agcgcgcgtg ctcgcgcacg tcggtgacac cgaagtcgtc accgtcgtgg
      481 gcgcgttcgg atatcgccca ctcccgttcg aagggcaatg ggcgcagcgc ccgattgttc
      541 ctccccccga tgcctcgcca ccgcggccat tgcggaacca ccacgaaggc accatcagta
      601 cacgcctcga cgcgcggctc gcggcgggcc gccccttctg ggagttcccc gccgagcagg
      661 gcagcggcac cgccgcgctg tgggtgcgca tgcccgagct gttggagatg tcggccgcct
      721 cactcgccat catcggcgac ttcgtgccga tgggcatcgg ccaggcgctc ggcgagcgcg
      781 cgggcgggaa cagcctcgac aacacgctgc gcgtcgcgca ccgggtcccg accgagtggg
      841 tgctggccga catccgcgtc catgcggttg ccgacgggtt cggtcacggg ctcgtgcacc
      901 tgtgggccga ggacggcacc ctgctcggca ctgcgagcca gtccacgatc gtgagagtct
      961 ggcgggagga acccgaggag acttcatgac tgtcccgcag cgttacggca tcacgttccc
     1021 gttcgacggc ctcccactcc tcgaacagcg cgagatcgtc cgccaactcg tcgacctcgg
     1081 ctacaccgac ctgtggtcgg ccgagtcggg cggctacgac gcgttcatcc cgctcgccgt
     1141 cgcggcgcag tgggcaccgg agctccggct cggcaccgcg atcgtgcccg cgtacacacg
     1201 cggcgcgcac acactcgcgt cgaccgtcgc gtcgatgtgc caggccgcgc cagggcagtt
     1261 cgcgctcggt ctcggcacgt catcgaacgt gatcgtcgaa cgatggaacg gcatcgcgtt
     1321 cgacaagccg taccagcgtg tgcgtgacac gatccgcttc ctgcgcaagg cgctcaccgg
     1381 tgagaaggtc accgaggaat acgagacgtt cgcggtgaac gggttccgcc tcgggatctc
     1441 ggtgcccgaa cagccgccca tcctcgtcgc cgcgctgcgc gaagggatgt tgcgcctcgc
     1501 cggccgcgag ggcgacgggg cgatcatcaa ctggctctcc gccgacgacg tcaagacggt
     1561 cgtgccgcac gtcggccccg acaaggagat cgcggcgcgc atcttcgtgc tgcccgtcga
     1621 cgacgcgggc atggcacgcg cggtgggcaa gcgcgcgatc gcgtcgtacc tcacggtgcc
     1681 ggtgtacgcg gccttccacg aatggctcgg ccggggcgac gagctgaagg acctatggcg
     1741 gctgtggaag gaaggcgacc gcaaggccgc ggccgactcg atccccgacc acgtcgtcga
     1801 ccagctgctc gtgtggggca cgccggcaca gtgccgcgag cacatccagc gctacataga
     1861 caacggcgtg acgacacccg cccccgcgtt gttcgtcgga cccgaacagc tccccggcat
     1921 catcaccgca ctgaagcgat agcgcccgcg catgttctcg gtcgaggact tcctctggta
     1981 cgtcgacgac gcgatcgacg gcatggtcgc gatcgtcacc gagttgggcg acgatctcgc
     2041 gaacacggcg atcgacacgc ccggcgccaa ctcaccgtac gtcgtgctca cgcactgcct
     2101 gggtgtcatg gagtggtggg gcggatactt catcgccggc cgcgccatcg accgcgatcg
     2161 cgacgccgag ttcgttgccc ggggctcggt tgccgacctc gcgcggcgtt cccgcgccgc
     2221 ccgcgaccag ctccgagcgg acctcgtcgc cttcgaccct cacgcgccac cggccggtac
     2281 acccgcgccc gagtgggccg acgaaccgtg cgcgcgtacg cagggcggaa ccgcgctgca
     2341 catctaccgc gagctcgcgc agcaccgcgg tcagatggag atcacgcgcg acgtgctgct
     2401 cacgagatga cgcgcagcat ctcctcgacc gtcgtctgac ccgccagcac gtgcgacatg
     2461 cccgagtcgc gcagcgtctg catcccttcg gcgatggcgt agcgctcgat gtcttgcgcg
     2521 ggcgcccgcg agagcacgag ttcgcggatg tcgtcgctca tgagcatgat ctcgtagcaa
     2581 ccgacgcggc cgtagaagcc ggtgccgtgg cactcgtcgc aaccgaccgc gcgccgcagt
     2641 tttgccgtct cccacgcttc ttcgggcacg cccatccggc gcaggatcgc ctccgggtcg
     2701 gggagcggag ccgcacacgc atcgcagagc cgccgcgcga gccgctgcgc gacgatgcag
     2761 gagatcgccg acgtcacaac gtagggctcc acgcccatct cgatgaagcg ggccggcgag
     2821 ctcgcagcgc tcgtggtgtg catcgtcgag aacacgaggt gacccgacat ggcggcaccc
     2881 gccgcgatcg tggccgtctc accgtcacgg atctcgccga cgagaatgat gtcggggtcg
     2941 ctgcgcagca ttgcccgcaa cgcgacgggg aacgtcagac ccgcgcgccg gttcacctgc
     3001 atctgcttga cgccccgcaa cttgtactcg accgggtcct cgaccgacat cacgctgcgg
     3061 tcgcgcgtgt cgagtgcggc caccgtcgag tacagcgtcg acgtcttgcc ggatccggtg
     3121 ggtccggtga ccagcaccgc cccttgcgcg gcttcgaagg ccgtcttgta gcgcgcgagt
     3181 tgttcgggcg cgatgccgag ccggctcaac tcgacgagac cggtcgaacg gtcgagcaag
     3241 cgcaacaccg cggcctcgcc gtacacggtc ggaaccgtcg cgacgcgcac gtcgacgtcg
     3301 tcgccgccga tccgttgcgt gaagcggccg tcctgcggca gtcgagattg cgcgatgtcg
     3361 agctccgcga gcaccttcac gcggctcagg atcggccgca gcaccgacgt gctcgcctcg
     3421 gagatgtcgt gcaacacacc atcgatgcgg aagcgcatct tcacgccgtc gcggcccggc
     3481 tcgatgtgga tgtcggaggc gcggtcgctg gcggcttctt cgagcaacga gtcgacgagg
     3541 cggacgatcg ggccttgctc gacgtcgtcg aggtcttcct cctcggcgaa ctcgtcgtcc
     3601 gtcgcgacgg cggcgaggtt ctcgcgtacg gcggaccgtg agtacgcctc ctcgatcgca
     3661 gccgccacct gtgccgcgtc ggccatgacc gcgatcatcg ggacgccgag ccgcatgcgc
     3721 aggtcgtcga gcaccatgac gtcgtccggg tcggccatcg cgagaaggat cgagccccgg
     3781 cggcggccga tcgggatcac ctgatgccgc cgcgcgatcg cctccggcac caggtcaccg
     3841 aagaccttcg ag
//
LOCUS       DASUGA010000352         2156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1687072, whole genome shotgun sequence.
ACCESSION   DASUGA010000352 DASUGA010000000
VERSION     DASUGA010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1687072"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /gene="sucD"
                     /locus_tag="VFX21_07045"
     CDS             complement(<1..765)
                     /gene="sucD"
                     /locus_tag="VFX21_07045"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX5095748.1"
                     /translation="MSIFVDENTKVVYQGLTGSQGRFHGLRNKAYGTQVVAGTNPKKA
                     GTDVEGVPIFATVGDAREATGANASCIFIPAPGVKDAVIEAAEAGIEFIVVITEGVPA
                     HDEAFFYNDLKERFPDTQLLGPNCPGIISPGKCNIGITPGEIARPGGPVGIVSRSGTL
                     TYQALHEMTQQGIGQTTCVGIGGDPVPGTSFIDCLERFEADPDTLAVMMIGEIGGSAE
                     EEAAEFIGANMSKPVVSYIAGVTAPPGKKMGHAGAII"
     gene            complement(777..1919)
                     /gene="sucC"
                     /locus_tag="VFX21_07050"
     CDS             complement(777..1919)
                     /gene="sucC"
                     /locus_tag="VFX21_07050"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010541245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX5095749.1"
                     /translation="MDLFEYQGKQLFARYGIPVSAGEAVTTVDDAVAAAERVGYPVVV
                     KAQVQVGGRGKAGGIKLANDAGETRTHASNILGMDIKGHVVKVLWIEHASDIAEEYYA
                     SFTLDRGAKKHLGMLSAQGGVDIEGVAAESPEAIARIHIDPVTGLTEAQAREWVAAAK
                     LNPRANDGTVDILLKLYNAYVDGDCDLVEINPLILTPEGKVHALDAKVTLDSNAEYRH
                     DDWEQYKATQELDEREQLAREKGLNYIGLEGSVGIIGNGAGLVMSTLDVVNQVGGSAA
                     NFLDVGGGADATVLANAIEVINTDEYVRAILVNIFGGITRCDEVANGIIAALGTVDVK
                     APIVIRLDGTNAEEGRALIKPHESDKLISQPTMLDAARKAVELAAK"
     gene            complement(1931..>2156)
                     /locus_tag="VFX21_07055"
     CDS             complement(1931..>2156)
                     /locus_tag="VFX21_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HEX5095750.1"
                     /translation="SGAHMTLVPKIVEEMRARGLADVVVFAGGIIPEDDVAALRDLGV
                     AEIFTPGAPLDGIGTWLESSLDSRETAASA"
BASE COUNT          300 a          724 c          723 g          409 t
ORIGIN      
        1 gatgatcgcg cccgcgtggc ccatcttctt gcccggcggc gcggtgacac cggcgatgta
       61 gctcaccacc ggcttcgaca tgttggcgcc gatgaactcg gccgcctcct cttccgcaga
      121 gccgccgatc tcgccgatca tcatcacggc caacgtgtcc gggtcggctt cgaagcgctc
      181 gaggcagtcg atgaagctgg tgccgggcac cgggtcgccg ccgatgccga cgcacgtcgt
      241 ctgaccgatg ccctgctgcg tcatctcgtg cagcgcctgg taggtgagcg tgcccgaacg
      301 ggacacgatg ccgaccgggc cgccgggacg cgcgatctcg ccgggcgtga tgccgatgtt
      361 gcacttgccc ggcgagatga tgcccggaca gttcggaccc aacagctgcg tgtccgggaa
      421 ccgttccttc aggtcgttgt agaagaacgc ttcgtcgtgc gcgggcacgc cctcggtgat
      481 cacgacgatg aactcgatgc cggcctcggc ggcttcgatc accgcgtcct tcacgcccgg
      541 agcgggaatg aagatgcacg acgcgttcgc gccggtggcc tcgcgtgcat ctccgacggt
      601 cgcgaagatc ggcacaccct cgacgtcggt gccggccttc ttcgggttgg tgcccgcgac
      661 gacctgcgtg ccgtacgcct tgttgcgcag cccgtggaac cggccctgac ttccggtcag
      721 tccttggtag acgaccttgg tgttctcatc gacaaagatc gacatgagcg cgtgacctac
      781 ttcgcggcga gctcaacggc cttgcgggcc gcgtcgagca tcgtgggctg ggagatgagc
      841 ttgtcggact cgtgcggctt gatcagcgcc cggccctctt cggcattggt gccgtcgagt
      901 cggatcacga tcggcgcttt cacgtcgaca gtgccgagtg cggcgatgat gccgttcgcg
      961 acctcgtcgc agcgcgtgat gccgccgaag atgttgacga ggatcgcccg cacgtattcg
     1021 tcggtgttga tgacctcgat cgcgttcgcg agcaccgtgg cgtccgcgcc gccgccgacg
     1081 tcgaggaagt tcgcggccga gccgccgact tggttgacga cgtcgagcgt gctcatcacg
     1141 agccccgcgc cgttgccgat gatgccgacg ctgccttcga gaccgatgta gttgaggccc
     1201 ttctcgcgcg cgagctgctc gcgttcgtcg agctcctgcg tcgccttgta ctgctcccaa
     1261 tcgtcgtggc ggtactcggc gttgctgtcg agcgtcacct tggcgtcgag cgcgtgcacc
     1321 ttgccttcgg gcgtgaggat gagcgggttg atctcgacga ggtcgcagtc gccgtcgacg
     1381 tacgcgttgt agagcttcaa caagatgtcg acggtgccgt cgttcgcccg cgggttgagc
     1441 ttcgcggccg cgacccactc gcgcgcttgc gcttcggtga gcccggtgac gggatcgatg
     1501 tgtatccgcg cgatcgcttc ggggctctcg gccgcgacac cttcgatgtc gacgccgccc
     1561 tgcgcgctca gcatgccgag gtgcttcttg gccccccggt cgagtgtgaa cgacgcgtag
     1621 tactcctcgg cgatgtcgga cgcgtgctcg atccacagca ccttcacgac atggcctttg
     1681 atgtccatcc cgaggatgtt ggatgcgtgc gtgcgggtct cgccggcgtc gttggcgagc
     1741 ttgatgccgc cggccttacc ccgcccgccg acctgcacct gcgccttgac caccaccggg
     1801 tacccgaccc gttccgcggc ggcgaccgcg tcgtcgaccg tcgtgacggc ctctccggcc
     1861 gatacgggga tgccgtagcg cgcgaagagc tgcttgcctt ggtattcgaa cagatccacg
     1921 ggcgtggacc ctacgcggac gcggcggttt ctcgcgagtc gagggaggat tccagccagg
     1981 tgccgatgcc gtcgaggggc gcgccgggcg tgaagatctc ggcgacacca aggtcgcgaa
     2041 gcgccgcgac gtcgtcctcg gggatgatgc cgcccgcgaa cacgaccacg tcggcgagcc
     2101 cgcgtgctcg catctcctcg acgatcttgg gcacgagcgt catgtgcgcg cccgag
//
LOCUS       DASUGA010000353         3213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2015722, whole genome shotgun sequence.
ACCESSION   DASUGA010000353 DASUGA010000000
VERSION     DASUGA010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2015722"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VFX21_07060"
     CDS             <1..461
                     /locus_tag="VFX21_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX5095751.1"
                     /translation="RRGPAELDEADKALIELLQTDGRMSYTRLAGEVGLSEAAVRQRV
                     QRLIEGNVVQVVAVTDPLRLGFRRQAMVGVKTEGDIAAIADLLAAIPEVDYVVFVAGS
                     YDLMFEIVCEDDDHLLSILNDKVRSIPGVRTTDTYTYLRLHKQTYAWGTR"
     gene            547..1911
                     /locus_tag="VFX21_07065"
     CDS             547..1911
                     /locus_tag="VFX21_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5095752.1"
                     /translation="MNNEEMQQAARDHLWLHFAKLGRYQDHELPIIVRGEGCNVYDQH
                     GDRKLDALSGLFVVQAGYGRRDLAEAGAHQAETLNYFPIWSYGHPPAIELAAKLAELA
                     PGDLNRVFFTIGGSEAVESAWKLARAYFRAIGQGQRYKVIARRTAYHGTTLGALAITG
                     LPALRAPFEPLTPGGVHARNTNRYRDPLAKDEAAFMDACASAIEDAIVFEGPETVAAV
                     FVEPVQNAGGCYTPPEGYFQRLREICDRYGVLLVSDEVICAYGRLGEMFGAQRYDYLP
                     DMITTAKGLTSGYSPLGAVICRDFLAEPFLDPNASFAHGLTFGGHPVGCAVALRNLQL
                     FEEEQFCEHVRTHEAGFKERLEGLYDLPIVGDVRGAGWFWAVELVKDQATEQPFTRPE
                     VEEVLRGIVAPALYKEGLICRADDRGDPVLQFAPPLIAGDKELDEIEAKVRTVLTDAA
                     AKAC"
     gene            1908..3023
                     /gene="ald"
                     /locus_tag="VFX21_07070"
     CDS             1908..3023
                     /gene="ald"
                     /locus_tag="VFX21_07070"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX5095753.1"
                     /translation="MTVGVPREVKTEEHRVAITPDGVNELTHRDVPVLVERDAGADSG
                     FANDDYASAGAEIVDQAADVWARAELVMKVKEPQPSEFVCLRPGLVLFTYLHLAAYPE
                     VARALLDAGTTGIAYETVQLPNGQLPLLAPMSEVAGRMAPQIGAHFLEKAHGGRGVLM
                     GGAPGVRPARVVVLGAGNVGWNAAWIAQGMEAEVLLLDKNLDRLRWVDQIHKGRIMTL
                     ASNRGAVTRAVTDADLVIGAVLVPGGRAPVLVSEAMVAGMKPGAVIVDCAIDQGGCIE
                     GIRETTHTDPVYEQYGVLHYAVGNVPGAVPNTSTYALTNATLPYALELATKGVKEAVD
                     ADPALALGVNTIDGCVPNTAVAQALGVQATPLGSLLR"
BASE COUNT          532 a         1079 c         1126 g          476 t
ORIGIN      
        1 aacggcgcgg cccggcggag ctcgacgagg ccgacaaggc gctcatcgag ctgctccaga
       61 ccgacggccg catgtcgtac acgcggctcg cgggcgaggt cgggctctcg gaagcggccg
      121 tgcgtcagcg cgtgcagcgg ctcatcgagg gcaacgtcgt ccaggtcgtc gcggtcaccg
      181 acccgctccg gctcgggttc cggcgccagg ccatggtcgg ggtcaagacc gagggcgaca
      241 tcgccgcgat cgccgacctg ctcgcagcca tccccgaagt cgactacgtc gtgttcgtcg
      301 cggggtcgta cgacctcatg ttcgagatcg tgtgtgaaga cgacgaccat ctcttgtcga
      361 tcctcaacga caaggtgcgg agcatccccg gtgtccgcac gaccgatacg tatacctatc
      421 tgcggctcca caagcagacg tacgcctggg gtacacgatg acgagtcagg cgagagatcc
      481 ggagggccgg tcgtggcact gacgtcgcac ggacattcga ggcccggagg tcggagcccc
      541 atgaacatga acaacgagga gatgcagcag gcggcgcggg atcacctgtg gctgcacttc
      601 gcgaagttgg gtcggtacca ggaccacgag ctgccgatca tcgttcgggg cgagggctgc
      661 aacgtctacg accagcacgg cgaccgcaag ctcgacgcgc tctcgggcct gttcgtcgtg
      721 caggccggct acgggcggcg cgacctcgcg gaagccggcg cacaccaggc cgagacgctc
      781 aactacttcc cgatctggtc gtacggccac ccgcccgcca tcgagctcgc ggcgaagctg
      841 gccgagctcg cgcccggcga cctgaaccgc gtgttcttca ccatcggtgg ctccgaggcc
      901 gtcgagtccg cgtggaagct cgcgcgcgcg tacttccgcg ccatcgggca ggggcagcgg
      961 tacaaggtca tcgcgcgccg caccgcgtac cacggcacga cgctcggtgc gctcgcgatc
     1021 accggtctgc cggcgttgcg cgcgccgttc gagccgctga ccccgggtgg tgtgcacgca
     1081 cgcaacacca accgctaccg cgatccgctg gccaaggacg aggccgcgtt catggatgcc
     1141 tgcgcgagcg cgatcgagga tgcgatcgtc ttcgaaggcc ccgagacggt cgcggccgtg
     1201 ttcgttgaac cggtgcagaa cgcgggtggg tgctacacgc cgcccgaggg atacttccaa
     1261 cggctgcgcg agatctgcga ccggtacggc gtcttgctcg tctccgacga agtgatctgc
     1321 gcgtacggcc ggctcggcga gatgttcggc gcgcagcgct acgactacct gcccgacatg
     1381 atcacgaccg ccaagggact gacgtcggga tactcgccgc tcggtgcggt catctgccgc
     1441 gacttcctcg ccgaaccgtt cctcgatccc aacgcgtcgt tcgcgcacgg cctgacgttc
     1501 ggcggccatc ctgtcggttg cgcggtcgcg ctgcgcaacc tgcaactgtt cgaggaagag
     1561 cagttctgcg agcacgtccg cacgcacgaa gccggcttca aggagcgact ggaaggtctg
     1621 tacgacctgc ccatcgtcgg cgacgtgcgc ggcgcagggt ggttctgggc cgtcgagctg
     1681 gtcaaggacc aggcgaccga gcaaccgttc acgcggccgg aggtcgaaga ggtgctgcgc
     1741 ggcatcgtcg cgcccgcgct ctacaaggaa ggcctgatct gtcgcgccga cgaccgcggt
     1801 gatcccgtcc tccagttcgc gccgccgctg atcgcgggcg acaaggagct cgacgagatc
     1861 gaagcgaagg tgcgcacggt actgaccgac gcggcggcga aggcgtgctg accgtcggtg
     1921 tcccgcgcga ggtcaagacc gaggagcacc gcgtcgcgat cacgcccgac ggcgtgaacg
     1981 agctgacgca ccgcgacgta ccggtgttgg tcgagcgcga cgcgggggcc gactcggggt
     2041 tcgccaacga cgactacgcc agcgccggcg cggagatcgt cgaccaggcc gccgacgtct
     2101 gggcgcgcgc cgaactcgtg atgaaggtga aggagccgca gccgtcggag ttcgtgtgcc
     2161 tgcgacccgg tctggtgctg ttcacctatc tccacctcgc cgcctacccg gaggtcgcgc
     2221 gcgcgctgct cgacgcgggc acgaccggca tcgcgtacga gacggtgcag ctcccgaacg
     2281 ggcagctgcc attgctcgcg ccgatgagcg aagtggccgg ccgcatggcg ccccagatcg
     2341 gcgcgcactt cctcgagaag gcgcacggtg gtcgcggcgt gctgatgggc ggcgcgcccg
     2401 gcgtgcgccc ggcgcgcgtc gtcgtgctcg gcgccggcaa cgtcggctgg aacgcggcgt
     2461 ggatcgcgca gggcatggag gccgaggtgt tgttgctcga caagaacctc gatcgcctgc
     2521 ggtgggtcga ccagatccac aagggccgca tcatgacgct ggcgagcaac cgcggcgcgg
     2581 tgacgcgcgc ggtgaccgac gccgacctgg tcatcggtgc ggtgctggtg cccggcggac
     2641 gcgcgccggt actggtcagc gaggccatgg tcgcggggat gaaaccaggc gccgtgatcg
     2701 tcgattgtgc gatcgatcaa ggtggctgca tcgaaggcat ccgcgagacc acccacaccg
     2761 atccggtcta cgagcaatac ggtgtgttgc actatgccgt cgggaacgtg ccgggcgcgg
     2821 tgcccaacac gtcgacgtac gcgttgacca acgcaacgct gccttacgca ctggaactcg
     2881 cgacgaaggg cgtcaaagag gccgtcgacg ccgacccggc gctggcgctc ggcgtcaaca
     2941 ccatcgacgg ctgtgtgccg aacacggccg tcgcgcaggc gctgggcgtg caagcgacac
     3001 cactcggatc actcctgcgg tgacgcccga cgagcttcag gcgttgcagt tgccctcgtc
     3061 gtagcgaggg tcgaatttcc aacccgcgtc ctcccaaccg ggacgatcga gtcggagatg
     3121 cgcgagcgtg aagtgtgtca gcgcggccgc gccatcggga cagagatgcc atccgtcagg
     3181 gccacgcaac cggcggccgt cgacgaactc ggg
//
LOCUS       DASUGA010000354         2394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_732925, whole genome shotgun sequence.
ACCESSION   DASUGA010000354 DASUGA010000000
VERSION     DASUGA010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732925"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2381)
                     /locus_tag="VFX21_07075"
     CDS             complement(<1..2381)
                     /locus_tag="VFX21_07075"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX5095754.1"
                     /translation="MSGRLLVTDREAETREPVIEIAHEALLTHWSRLRDWVEADRQWL
                     RQLQHLALATHTWDDAGRPDAELYRGARLEAAIEALPDRRGELTPNEIAFVEAGQRVR
                     DADREQERRVARRTRRLLGVVAGALVIALIAGAVAFLQRGNARAAADAATDAQREAEI
                     EALIGRIETLRSTQRDAAALLAIEAFKLADTPRTRAALLSTFTENPGYLDTHQLPPGN
                     DLYFGVVMDDGKHAFVTTRDHVIRPYDLETGELGKPWPVMANKPLPFSWLQGSPDGTS
                     LAQNSDNESQTGVAASTIGVFDVANGALRFPALQLPIGIGIILFSPDGKLLAAAGGTD
                     ETVTVFDAFTGRELGRYRGLDQPGDIRWGTAGISFLGNRQLAIGSIQGRVRIVDVPSM
                     RVAREFPVPDATTQELSTLDGGRALLGIGLNGLIRFDATTGARTWFVPESRDSCSMYT
                     VIESRNRVYCSDVFGRLDERDLGDGHVVRQLDAQKGAAGSLWPARNDTELVEFGNGAT
                     VVTRWRLDGTGPITRRLEPGAVASRYSPDGSQLIGLKVVGDARQFEPKLADAALLDAR
                     TGKVVDELEGIVSPGWITPDRLGGAVMDGDDFVLTQYDPVSKRRSPGIRLPGEGLSGG
                     LNVGTGKTWMFYDRDKGKTEIQTIDVKTSRRVEPTFTVPSFAKADASADGSRFVVAAE
                     DIIVYDTTTGKEVGRIKGHFLNGVHLTRNNVVFAWSLGGALKVYDLKTLKPIRELAGN
                     RGYLVDVKSDARGGTIAASGGDRQVTLYDAATGVALGESITVADEESTLIALRP"
BASE COUNT          372 a          802 c          793 g          427 t
ORIGIN      
        1 ggtcgcaacg cgatcaatgt cgactcctcg tcggcgaccg tgatcgattc gcccagcgcg
       61 actccggttg ccgcgtcgta caacgtgacc tgccggtctc cgccgctcgc cgcgatcgtc
      121 ccgccgcgcg cgtccgattt cacgtcgacg agatagccac ggttgcccgc gagttcacgg
      181 atcggcttca gggtcttcag gtcgtacacc ttgagcgcgc cgcccaacga ccacgcgaac
      241 acgacattgt tcctggtgag atgtacgccg ttcaggaagt gtcccttgat gcgtccgact
      301 tctttgcccg tcgtcgtgtc gtacacgatg atgtcctcgg ccgcgacaac gaaccggctg
      361 ccgtcggcag aggcgtcggc cttggcgaac gacgggacgg tgaacgtggg ctcgacccgc
      421 cggctcgtct tgacgtcgat ggtctggatc tcggttttcc ccttgtcgcg gtcgtagaac
      481 atccacgtct tcccggtgcc gacgttcagg ccgccgctca gcccttcccc gggaaggcga
      541 atgccgggcg aacgtcgttt ggagacgggg tcgtattggg tcagcacgaa gtcgtcgccg
      601 tccatgactg cgccgcccag ccggtcgggc gtgatccaac ccggcgaaac aattccctcg
      661 agttcgtcga cgaccttgcc tgtgcgtgcg tcgagcagcg cagcgtccgc aagtttcggt
      721 tcgaactggc gcgcgtcgcc gacgactttg aggccgatga gttgcgagcc gtcggggctg
      781 tacctcgacg cgacggcgcc gggttcgaga cgccgcgtga tcggacccgt tccgtcaaga
      841 cgccaccgcg tcaccaccgt tgcgccgttg ccgaactcga ccagctccgt gtcgttgcga
      901 gccggccata acgaacccgc cgcgcccttc tgtgcgtcga gctgacgcac gacatggccg
      961 tcgcccagat cgcgctcgtc cagccggccg aagacgtcgg agcagtagac gcgattccgt
     1021 gactcgatga cggtgtacat ggagcagctg tcgcgtgact ccggcacgaa ccacgttcgg
     1081 gcaccggtcg tcgcatcgaa gcggatgagg ccgttgagac cgatgcctag caacgcccgc
     1141 ccgccgtcga gcgtcgagag ttcttgtgtc gtcgcgtcgg gcaccgggaa ctcgcgcgcg
     1201 acgcgcatgc tcggcacgtc gacgatgcgc acgcgtccct ggattgagcc gatcgccagt
     1261 tgccgattgc cgaggaagct gatgccggcg gtgccccacc gaatgtcacc gggctggtcg
     1321 agcccgcggt agcggccgag ctctcgtccg gtgaacgcgt cgaagacggt aacggtttcg
     1381 tcggtaccgc ctgccgccgc caggagcttc ccgtccgggc tgaagaggat aattccgatc
     1441 ccgatcggca actggagcgc ggggaagcga agcgccccgt tcgcgacgtc gaacacgccg
     1501 atggtcgacg ccgcgacccc cgtctgagat tcgttgtcgg agttctgggc caaggatgtg
     1561 ccgtccgggc tcccctgcaa ccagctgaac ggcagcggct tgtttgccat gacgggccag
     1621 ggcttgccca actcgcccgt ctcgaggtcg tacggacgga tgacgtgatc gcgcgtcgtc
     1681 acgaacgcgt gcttgccgtc gtccatcacg actccgaaat acaagtcgtt cccgggcggc
     1741 aattgatgcg tgtcgaggta accgggattc tcggtgaaag tggaaagcag cgccgcgcga
     1801 gtgcgcggtg tgtccgcgag cttgaaggct tcgatcgcca gcaacgccgc cgcatcgcgc
     1861 tgggttgatc gcaaggtctc gatgcggccg atcagcgctt cgatctctgc ttcgcgttgt
     1921 gcatcggtgg cggcgtcggc cgcggcccga gcattgccgc gctgtaggaa cgcgaccgcg
     1981 cccgcgatca acgcgatcac gagcgcgccg gcaacaacgc cgaggagcct gcgagtgcgc
     2041 cgggcgacgc gccgttcttg ctcgcgatcg gcgtcgcgca ctcgctgacc ggcttcgacg
     2101 aacgcgatct cgttcggcgt gagttcaccg cgccggtcgg gcagcgcttc tatggccgct
     2161 tcgaggcgcg cgccgcggta caactctgcg tcgggccggc ccgcgtcgtc ccaggtgtgt
     2221 gtggccaggg cgaggtgctg caactgtcgc aaccattggc gatccgcttc gacccagtca
     2281 cgcagccgcg accaatgggt gagcagtgcc tcgtgcgcaa tctcgatgac gggctcgcgc
     2341 gtctccgctt cgcggtcggt cacgaggagg cgcccggaca cgaaactggc gacc
//
LOCUS       DASUGA010000355         2321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_181990, whole genome shotgun sequence.
ACCESSION   DASUGA010000355 DASUGA010000000
VERSION     DASUGA010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181990"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VFX21_07080"
     CDS             complement(<1..335)
                     /locus_tag="VFX21_07080"
                     /inference="COORDINATES: protein motif:HMM:NF018766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HEX5095755.1"
                     /translation="MGEVHDHLIHGFMYWNLMLALVPVGLAFLLFRPGTVRTPAWWAG
                     CALWVLFLPNAPYVLTDVVHMVDDIQAARASNPRAYTIVVLYALFFAAGMVCYVLAIG
                     LLERYLAPQ"
     gene            complement(351..1181)
                     /locus_tag="VFX21_07085"
     CDS             complement(351..1181)
                     /locus_tag="VFX21_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095756.1"
                     /translation="MAGGEHVVLVVHGVANRTPADFQDEIDALNPALAPRAIVPVFWG
                     DLGPADATASFPVIEGSIAIDRGDDDDADSSLRARLRRITDAVDALSPESFATGLRRT
                     IARTVRDVEAYERNGESIRARLDEAYRTARGDAERVDVVAHSLGGLVAVEWLFGAPAG
                     NGATAPGERRIHTLVTFGTQVGLFCELRGLQGPPGTIALPPAPISLTLALRRWCNVWH
                     ALDPLAFAAAPAFTVAGRDGPVGIEDYRLPVHGVPESAAFHTAYWRDLHFGAWLDRTL
                     "
     gene            1212..>2321
                     /locus_tag="VFX21_07090"
     CDS             1212..>2321
                     /locus_tag="VFX21_07090"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX5095757.1"
                     /translation="MTDLSILDATAQAELVRTGDATAAELVDSAIERIEKLNGELNAV
                     IHPLYDRARAAVAAGLPSGPFTGVPIVIKDLDGTLADAPYHGGNKMLKAANYVPSVTS
                     YIFEKLERAGFVIVGKTNTPEFGLMTTTESEAYGPAHNPWDVTRSTGGSSGGSGAAVA
                     SGMVPVAHAGDGGGSIRIPASHCGLFGLKPNRGRVSLGPDEAEAWAGLVMRHVVSHTV
                     RDSAAILDCIHGYMNGDYYTAPPPGRAYARDAMNEPGTLRIGIRTTAPLGLAEVDAEC
                     VAAAENAAALCESLGHNVEVATPGALDDFSMMESFLAVMNSSLRADLQMLGEQLGREV
                     TAADVEAGTWSNYESASGIDAAAYIRALTKMHQWVR"
BASE COUNT          424 a          810 c          764 g          323 t
ORIGIN      
        1 atttgcggcg cgagatagcg ctccagcaag ccgatcgcca ggacgtaaca caccatgccg
       61 gcggcgaaga acaacgcata gagcacgacg atcgtgtacg cgcgcgggtt cgaggcacgc
      121 gcggcttgga tgtcgtcgac catgtgcacc acatcggtca atacgtacgg cgcgttgggc
      181 aggaacaaca cccacagcgc gcatccggcc caccatgccg gtgtgcgaac ggtgccgggc
      241 cggaagagca agaacgcgag tccgaccggc acgagcgcca gcatcaggtt ccagtacatg
      301 aaaccgtgaa tgagatgatc gtgcacttca cccatggggg cgacggtacg tcacagtgtg
      361 cggtcgagcc acgcgccgaa gtgaagatcc cgccaatacg cggtgtgaaa cgccgctgac
      421 tccgggacgc cgtggacggg caggcggtag tcctcgatcc cgaccgggcc gtcgcggccg
      481 gcgaccgtga acgccggcgc cgccgcgaac gcaagcggat cgagcgcgtg ccagacgttg
      541 caccagcgcc gcaacgcgag cgtcagcgag atcggcgccg gtggcagcgc gatcgttccc
      601 ggcgggcctt gcagtccgcg cagctcgcaa aacaggccga cctgggtccc gaacgtgacc
      661 aacgtgtgga tgcggcgttc gccgggtgcg gttgcgccgt tcccagccgg cgcaccgaac
      721 agccactcga ccgcaacgag tccacccagt gagtgcgcaa cgacgtcgac acgttcggcg
      781 tcgccgcgag ccgttcggta cgcctcgtcg agccgcgccc ggatcgactc gccgttgcgt
      841 tcgtacgcct cgacgtcgcg gaccgtacgc gcaatcgtgc gtcgcaatcc ggtcgcgaac
      901 gactccggcg aaagcgcatc gaccgcgtcg gtgatccgcc gcagccgcgc gcgcagcgac
      961 gagtcggcgt cgtcatcgtc gcccctatcg atcgcgatcg atccctcgat gacgggaaac
     1021 gacgcggttg catcggcggg cccgaggtcg ccccagaaca cgggcacgat cgcacgaggt
     1081 gcgagcgccg ggttcagggc gtcgatctcg tcctggaaat cggccggcgt tcggttcgcg
     1141 acaccgtgca cgacgagcac gacgtgctca ccccctgcca ttgccgcatc ctacgaacta
     1201 gcgtccggcg cgtgaccgac ctctccatct tggacgccac cgcgcaagcc gaactcgttc
     1261 gcacgggcga cgcaacggcc gccgaactcg tcgacagcgc catcgaacgc atcgagaagc
     1321 tgaacggcga gctgaacgcc gtcatccacc cgctctacga ccgtgctcgc gccgcagtcg
     1381 cagcaggcct gccatccggc ccgttcaccg gcgttccgat cgtgatcaag gatctcgacg
     1441 gcacgctcgc ggacgcgccg tatcacggtg gcaacaagat gctgaaggcg gcgaactatg
     1501 tgccgtcggt gacgagctac atcttcgaga agctcgaacg cgccggattc gtgatcgtcg
     1561 gcaagaccaa cacacccgag ttcggactca tgaccacgac cgaatccgaa gcctacggtc
     1621 ccgcgcacaa cccgtgggat gtcacgcgca gcaccggtgg atcgagtggc ggctcgggtg
     1681 ccgcagtcgc gagcggcatg gtcccggtcg cgcacgccgg cgacggtggc ggctcgatcc
     1741 gcatcccggc ctcgcactgc ggactcttcg gcttgaaacc gaaccggggc cgtgtttcgc
     1801 tcggacccga cgaagccgag gcctgggccg gtctcgtgat gcgccacgtc gtgtcgcaca
     1861 cggtccgcga cagcgccgcc atcctggatt gcattcacgg ctacatgaac ggcgactact
     1921 acaccgcgcc tccgcccggc cgcgcgtacg cgcgcgacgc gatgaacgaa ccgggcacct
     1981 tgcgcatcgg catccgcacg accgccccgc tcggcctggc cgaggtcgac gccgaatgcg
     2041 tggccgcggc ggagaacgcc gcggcgctgt gcgaatcact cggacacaac gtggaagtcg
     2101 cgacgccggg cgcgctcgac gacttctcga tgatggagtc gttcctggcg gtgatgaact
     2161 cgtcgctgcg cgccgacctg cagatgctcg gcgagcaact cggccgcgag gtcaccgcgg
     2221 ccgatgtcga ggccggcacc tggagcaact acgaaagcgc gtcgggaatc gacgccgccg
     2281 cgtacatccg cgcgctgacg aagatgcacc agtgggtgcg c
//
LOCUS       DASUGA010000356         2364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_353814, whole genome shotgun sequence.
ACCESSION   DASUGA010000356 DASUGA010000000
VERSION     DASUGA010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353814"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2364)
                     /locus_tag="VFX21_07095"
     CDS             complement(<1..>2364)
                     /locus_tag="VFX21_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5095758.1"
                     /translation="PGPPGAYPDLDPTSDDFCKLAIDLGTAGSVVVAPDGSVLVAASG
                     ALRVDRFNPPFPTSPDAAGGCGAVDPQGSPLVDASITNRAPGATPFRETNVLAPGGGM
                     LTPSGLALSPQGTLYAASVLTGAINEYDLSTGALLRTLVEAPNPLSLPTPYGSPQGIA
                     VGPDGTLYYADLNLVGTLSDPSTIGPGPNGKVWRVRFDGNGDPMTPEMVRDGLGFPDG
                     VALFPGNLQQSEWLTFAGSAARKFDNPAESVITEGNVDQLAIRWQIPVTKVITGSPTV
                     AAIDLPGEGLRQVVFFQSWDLAIYAARLSDGSVIWRVETDDQPGASFPSTASVHVAKL
                     ANRDTVLVGQGHNFFALDATTGAEVWRFTAGTGCGYDDGGPPGLCGFAGERNQIESSA
                     IVANGLVFFGMDVNDVATGKGGFYALDALDGRMRWFFDLESGMTCRPDPGDEIRKYDG
                     YHSEAQLGLPAGFFATRAGCNHPRSANGCSNVWSSPAADEGRGALFVASSNCDMPIDP
                     MSGDPFPMPPFDEAIFSLDFAGNVRWVWRPREFDNDDLAFGAAPNLFQITVDVAAVPT
                     LVDVVGIGGKDGTYYVLDRDGVNRVTGERWDDDPASHLPAALPYWETNVVDGGDIGGI
                     LSTAAVDEANHRIHFSTAPGESGVNSPPATPQLPTVHTLDMDDGSVLWQNAGEMPPLA
                     SFSSVSHIPGVVVAGTSLGAKLRSYADDAGTKLGEFDLANFGIGSTPVFVDGTVLVGA
                     GIGTRTTTGSGISDVVANFPSDLTALCVPGTSGCSACDDGEDNDGDG"
BASE COUNT          330 a          820 c          863 g          351 t
ORIGIN      
        1 gcccgtcgcc gtcgttgtcc tcgccgtcgt cgcaggcgct gcagcccgac gtgcccggca
       61 cgcacagcgc ggtcaggtcg gacggaaagt tcgcgacgac gtcgctgatg ccgctgccgg
      121 tcgtcgtgcg ggttccgatc ccggcgccga cgagcacggt gccgtccacg aacaccggcg
      181 tcgatccgat gccgaagttc gcgaggtcga actcgccgag cttcgtcccg gcgtcgtccg
      241 cgtagctgcg cagcttcgcg ccgagcgacg tgccggcgac gacgacgccc gggatgtggc
      301 tcaccgacga gaagctcgcg agcggcggca tctcgccggc gttctgccac agcacggagc
      361 cgtcgtccat gtcgagcgtg tggacggtcg gcagttgcgg agtggcgggc ggcgagttca
      421 cgccgctctc gccgggtgcg gtgctgaagt ggatgcggtg gttcgcctcg tcgaccgccg
      481 cggtcgagag gatgccgccg atgtctccgc cgtcgacgac gttcgtctcc cagtacggca
      541 gcgccgcggg caggtggctc gccggatcgt cgtcccagcg ctcgccggtc acgcggttga
      601 cgccgtcgcg atcgagcacg tagtaggtgc cgtccttgcc gccgattccg acgacgtcga
      661 cgagggtcgg caccgccgcg acgtcgaccg tgatctggaa caggttcggc gccgcgccga
      721 acgcgagatc gtcgttgtcg aactcgcgcg gccgccacac ccagcgcacg ttgcccgcga
      781 agtcgagcga gaagatcgcc tcgtcgaacg gcggcatcgg gaacggatcg ccgctcatcg
      841 ggtcgatcgg catgtcgcag ttgctcgacg cgacgaacaa cgcgccgcgc ccctcgtcgg
      901 ccgcgggcga cgaccacacg ttgctgcagc cgttcgccga ccgcgggtgg ttgcagcccg
      961 cacgcgtcgc gaagaagccg gccggcagcc cgagctgcgc ctcgctgtgg tagccgtcgt
     1021 acttccggat ctcgtcaccg ggatcgggcc ggcaggtcat gccgctctcg aggtcgaaga
     1081 accagcgcat gcggccgtcg agcgcgtcga gcgcgtagaa gccgcccttg ccggtcgcga
     1141 cgtcgttcac gtccatgccg aagaacacga ggccgttcgc gacgatggcc gacgactcga
     1201 tctggttgcg ctcgcccgcg aagccgcaca ggccgggcgg gccgccgtcg tcgtagccgc
     1261 agccggttcc ggccgtgaag cgccacacct cggcgcccgt cgtcgcgtcg agtgcgaaga
     1321 agttgtggcc ctggccgacg agcaccgtat cgcggttcgc gagcttcgcg acgtgcacgg
     1381 acgcggtgct cgggaacgac gcgcccggct ggtcgtcggt ctcgacgcgc cagatcacgc
     1441 tgccgtccga gaggcgcgcc gcgtagatcg cgagatccca ggactggaag aatacgacct
     1501 ggcgcagccc ttcgccgggc agatcgatcg cggcgacggt cggcgatccg gtgatcacct
     1561 tcgtgaccgg gatctgccag cggatcgcga gctggtcgac gttgccttcg gtgatcacgg
     1621 attccgcggg attgtcgaac ttgcgcgccg cgctgccggc gaacgtcagc cactcgctct
     1681 gctgcaggtt gccggggaac agcgccacgc cgtccgggaa gccgaggccg tcgcggacca
     1741 tctcgggcgt catcgggtcg ccgttcccgt cgaagcgcac gcgccacacc ttgccgttgg
     1801 gaccgggccc gatcgtgctc ggatcggaga gggtgccgac gaggttcaga tccgcgtagt
     1861 agagcgtgcc gtccgggccg accgcgatgc cctgcggcga gccgtagggc gtcggaagcg
     1921 agagcgggtt cggcgcttcg acgagcgttc gcagcagcgc tcccgtcgag aggtcgtact
     1981 cgttgatggc gcccgtgagc acgctggccg cgtacagcgt gccctgcggc gacagtgcga
     2041 ggccggacgg cgtcagcatg cctccgcccg gcgcgagcac gttggtctcg cggaatggcg
     2101 tcgcgcccgg cgctcggttc gtgatgctcg cgtcgacgag cggagagccc tgcggatcga
     2161 cggccccgca gccgccggcc gcgtcgggcg aggtggggaa gggcgggttg aagcggtcga
     2221 cgcgcagcgc gcccgacgca gcgaccagca cgctcccgtc cggcgcgacg accacgctgc
     2281 ccgcggtgcc gaggtcgatc gcgagcttgc agaagtcgtc gctcgtcgga tcgagatccg
     2341 ggtacgcgcc cggcggcccc gggt
//
LOCUS       DASUGA010000357         2167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1127793, whole genome shotgun sequence.
ACCESSION   DASUGA010000357 DASUGA010000000
VERSION     DASUGA010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127793"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VFX21_07100"
     CDS             complement(<1..964)
                     /locus_tag="VFX21_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095759.1"
                     /translation="MSGLISPSMLTRSRTVADPRWSPSGALLGWVDGYDGRSDVVVMP
                     ADGSSPPIVVTGDCGAGGGWAWVSDDELVVGAANGRLVLVSAEGGVIRELTQDHRAFS
                     PSVSVRGEVAFSLERADACDIATVPLDGSAWPVRVSDASYAWDPAWSPDGRELAWHEW
                     DLPAMSWDQSRIVVRDDDGKTRVVADAPACGQPRWSVDGKLAYIASNLLHVDGAPLLE
                     ESFEHAEPAWGPGARSYAWSPDGSELAWCRNEDGFGRLVIGKIGRKSARELSKGWHRG
                     IAWGDDGIVCVRSGGVTPSQVVVLAPNGSARRTIARGPVGGFERT"
     gene            1076..>2167
                     /locus_tag="VFX21_07105"
     CDS             1076..>2167
                     /locus_tag="VFX21_07105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5095760.1"
                     /translation="MIYRGNPHCGGQGVYSRHLTRELTELGHDVTMLAGQPWPVADAP
                     VRLEQVPGLDLYKRENPFRVPWLYEFRSWEDVEEFGIMAAAGYPEPYAFSRRVYKQLK
                     FRRHEFDLVHDNQCLGRGMLGFLRDGWPFVHTLHHPITVDRDLDLSHASSLHRKLTLR
                     RWYGFLGMQMQVARQLPRHITVSENSKKDIVAQMGVDPATLHIVPVGVDQRQFRPMPH
                     VKKVKGRLMTTASADVPLKGLTYLIEALAKIRTEREDAHLVVIGQPRHKSAVPAQIER
                     LGLQGAIEFVSGVSDERIVELYAEAEIAVVPSLYEGFSLPAIEAMACGVPLVTTTGGA
                     LPEVVGPSGGAAMTVPTADPGALAQQIVDV"
BASE COUNT          349 a          787 c          707 g          324 t
ORIGIN      
        1 ccgtgcgttc gaagccgccg accggtccgc gcgcgatcgt gcggcgcgcc gaaccgttcg
       61 gggcgagcac gacgacctgc gacggcgtca cgccgcccga gcgcacgcac acgatgccgt
      121 cgtcgcccca cgcgatgccg cggtgccagc ccttcgacaa ttcgcgcgcc gacttgcggc
      181 cgatcttccc gatcacgagc cggccgaagc cgtcttcgtt gcgacaccat gccagttccg
      241 aaccgtcggg cgaccacgcg tacgaacgcg cgcccggacc ccacgccggc tccgcatgct
      301 cgaacgattc ttccaagagc ggcgcgccgt cgacgtgcag caggttcgac gcgatgtacg
      361 cgagcttccc gtcgaccgac cagcggggct ggccgcacgc gggcgcgtcg gcaaccacgc
      421 gcgtcttgcc gtcgtcgtca cgcaccacga tgcgggactg gtcccacgac atcgcgggca
      481 gatcccattc gtgccacgcg agctctcgcc cgtccggcga ccacgccggg tcccacgcat
      541 agctcgcatc ggacacccgt accggccagg ccgacccgtc gaggggcacg gtcgcgatgt
      601 cgcacgcgtc ggcgcgttcg agcgagaacg cgacctcgcc ccgcaccgag accgacggcg
      661 agaatgcccg atggtcctgc gtgagttcgc ggatgacacc gccctcggcc gagaccagca
      721 cgagccggcc gttggcggct ccgaccacca gctcgtcgtc ggagacccac gcccagccgc
      781 caccggcgcc acagtcgccg gtcacgacga tcggagggct cgacccgtcg gcgggcatca
      841 cgaccacgtc ggagcggccg tcgtagccgt cgacccagcc gagaagggcg cccgagggtg
      901 accagcgagg atcggccacc gtacgggagc gggtcagcat ggagggtgag atcaggcccg
      961 acaccagggc aacctactgc gcaccggctt gctattcgag gggccttccc tccatcctgg
     1021 tttcttcatg ccgagcaccg attcccccct ttcgcgcccg cttcgcgtcg cgttcatgat
     1081 ctaccgcggc aacccgcatt gcgggggcca gggagtctac agccgccatc tcacgcggga
     1141 gctgacggag ctcggccacg acgtcaccat gctcgccggc cagccctggc ccgtcgccga
     1201 cgcgccggtg cggctcgagc aggtgcccgg cctcgacctg tacaagcgcg agaacccgtt
     1261 tcgggtcccg tggctctatg agttccgctc ctgggaggac gtcgaggagt tcgggatcat
     1321 ggccgcagcc ggctaccccg agccctacgc attcagccgc cgggtctaca agcagctgaa
     1381 gttccgtcgc cacgagttcg acctcgtgca cgacaaccag tgcctcggac gcgggatgct
     1441 cgggttcctg cgcgacggct ggccgttcgt gcacacgttg caccacccca tcaccgtcga
     1501 ccgcgatctc gacctgtcgc acgcgtcgtc gctgcaccgc aagctcacgc tgcgccgctg
     1561 gtacgggttc ctgggcatgc agatgcaggt cgcgcgccag ctgccgcggc acatcacggt
     1621 gtcggagaac tcgaaaaagg acatcgtcgc ccagatgggt gtcgaccccg cgacgttgca
     1681 catcgtgccg gtcggtgtcg accagcgtca gttccggccg atgccgcacg tgaagaaggt
     1741 caagggccgc ttgatgacga ccgcgagcgc cgacgtgccg ctcaagggtc tgacgtatct
     1801 catcgaagcg ctcgcgaaga ttcgtaccga gcgcgaagac gcgcacctcg tcgtgatcgg
     1861 ccagccccgt cacaagtcgg cggtgccggc gcagatcgaa cgcctcggac tgcaaggcgc
     1921 gatcgagttc gtgtcgggtg tgagcgacga gcgcatcgtc gagctctacg ccgaggccga
     1981 aattgcggtg gttccttcgc tttacgaggg tttttcgttg cctgcaatcg aggcaatggc
     2041 atgcggcgtt ccgctcgtga ccacgacggg cggtgcactg cccgaggtcg tcggcccgag
     2101 cggtggcgcg gcgatgaccg tgccgaccgc cgaccccggc gcactcgcgc aacagattgt
     2161 cgacgta
//
LOCUS       DASUGA010000358         4354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1502140, whole genome shotgun sequence.
ACCESSION   DASUGA010000358 DASUGA010000000
VERSION     DASUGA010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502140"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /locus_tag="VFX21_07110"
     CDS             <1..1545
                     /locus_tag="VFX21_07110"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5095761.1"
                     /translation="LEVEGADASAYTFTAFTIAYTLYLVSRSMGIANTTAGAREPHLL
                     ATTTRRTMRETARIIIPAVAFIVVFAPWILLVFGKDYSEGGTTLLRLFALSAIPYLVT
                     STFVSVSYVQRRMRAVIATTGSMAVSTLVLSVSLLIAFGVNGVGVGWLLAQSGVALVL
                     LCTEFRSLWVSRVDVRRLEPVVGGARALAARRRAPAVRERATQVVADDERLRDWTVER
                     VLPSEHDVDIIALRGEDDERALLRLPQSPSGTRAVAGHTGALRQLRERDIASLRPLVP
                     EVLAENLSGNAPWMLESHLPGAPASGFLDVFGTERGLDMVRSVIEPLHAHALTTMVAD
                     DAALEAWVDRPIEQVRSVPGPALYRHRDEAALDRLREELHDALRGREVAAGFVHGDLW
                     LGNVLVDPDEHEVCGVIDWDFAREPVPVQTDVMHLILATRSAREKMELGDVVVRLLAT
                     DAWEDYELDTLARTGGLGACDVPPRTLLLLTWLGHVSGLPDKSDRYRKSLVWRARNTE
                     QVLERL"
     gene            1503..3401
                     /locus_tag="VFX21_07115"
     CDS             1503..3401
                     /locus_tag="VFX21_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095762.1"
                     /translation="MASTQHRASAGTTLTVLDAPLAEPIREPGPAVAPVERRAPWIFP
                     VVMLTAALACWIVALRNIDPRDIGDYGLISAFPPAMYLAVAILTLSFCVVLNTRVRDE
                     LLGAHVVLLVLIIHATPSITYGTLRYAWAWKHLGIVDYFHRNHALWPESPTLTVYHNW
                     PGFFTFVTHLTEASGLASAGSFAAWAPPFFELLFVLAVAAVISALTSDRRVIALGVWL
                     FVLANWVGQDYFAPQAFAYFLYLVLFAILLRWFRNEPAPKYFKGLRHRLQRRVGRALV
                     EDPPHPVTVPEAQQRTPLLFFVVLMMIAIATSHPLTPFVAIAGLLALAVTRTISVRVL
                     PLVMATMTLAWLCTGAWPYVSPNARALFSEIGALGANVDSNLRNTSVVSDAQQLVSAA
                     GRYVVVSMVALAALGVLRRLRVGRFEGAAISLMAAPIAVAIAGSYGGEVIFRVFLFAL
                     PFAAYLAARAFMSSDDGGRGILTTLALIVVSGVLLAGSFLAYYGKEQWTHFSTDEVRA
                     AQAVFDNAPDDSLLIAFDRSMPYQFANYDRFTYVWILEEPSSSVRRILNDPVGSLEDW
                     MEQKEYRRSYLLISRAQSEETAALGVLPAGTLDTVRDRLKASGVFDIVYDGPDATVFS
                     LDPHAVTP"
     gene            3398..3730
                     /locus_tag="VFX21_07120"
     CDS             3398..3730
                     /locus_tag="VFX21_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095763.1"
                     /translation="MNRRLVCAVWALAAVAVTGLEAPRAIAFPVMFGFLCFVPGFALV
                     SRLRLQSLGIEVAIAFGVSMALAAIVTEVLMYAELYSALPATLTLALITLTACAWPVS
                     RTRASQLT"
     gene            complement(3718..>4354)
                     /locus_tag="VFX21_07125"
     CDS             complement(3718..>4354)
                     /locus_tag="VFX21_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HEX5095764.1"
                     /translation="FIQIYGLTETTPLLTMNRGREEYDALSPADRAARLGRAGAPALG
                     VRLAVDAEGEVLARTNHVMEGYWEQPDETAKAIRDGWFRTGDGGLIDDESYLSIADRK
                     KDVIISGGENVSSIEVEDVVFSHPAVAEVCVIGVPDEKWGETVIALVVLAPGVSATED
                     ELIAHCRDRLTHFKCPTRIEFRDVLARTATGKLQKFKLRAPYWEGRERQVN"
BASE COUNT          609 a         1507 c         1459 g          779 t
ORIGIN      
        1 ctcgaggtcg agggcgccga cgccagcgcg tacacgttca ccgcgttcac gatcgcctac
       61 acgctgtacc tggtcagccg cagcatgggt atcgcgaaca cgaccgcggg cgcgcgcgaa
      121 ccgcacctgc tggcgacgac gacccgccgc acgatgcgcg agaccgctcg gatcatcatt
      181 cccgcggtcg cgttcatcgt cgtcttcgcg ccgtggatcc tgctcgtctt cggcaaggac
      241 tactcggagg gcggcacgac gctgctgcgg ctgttcgcgc tgtccgccat cccgtacctc
      301 gtcacgagca cgttcgtcag cgtctcgtac gtacagcggc gcatgcgcgc cgtcatcgcc
      361 acgaccggat cgatggccgt gtccacgctc gtcctctcgg tctcgctgtt gatcgcgttc
      421 ggcgtgaacg gcgtgggcgt gggctggctc ctcgcgcagt caggcgtggc cctcgtcttg
      481 ctctgcaccg agttccggtc gctgtgggtg tcgcgcgtcg acgtgcgccg cttggagccc
      541 gtagtcggcg gcgcgcgtgc gctggcggcc cgccgacgcg cgcccgccgt tcgcgagcgg
      601 gcgacgcagg tcgtggccga cgacgaacgg ctgcgagact ggaccgtcga gcgcgtgctg
      661 ccgagcgagc acgacgtcga catcatcgcg ttgcgcggcg aagacgacga gcgtgcgctg
      721 ctccggctcc cgcaatcacc gagcgggacg cgcgcggtcg cgggtcacac cggcgcgctc
      781 cggcagctgc gcgagcgcga catcgcgtcg ctgcgaccgc tcgtccccga ggtgctcgcc
      841 gagaacttgt cgggcaacgc gccgtggatg ctggagtcgc acctgcccgg tgcgccggcg
      901 tcggggttcc tcgacgtgtt cgggacggaa cggggcctcg acatggtccg cagcgtgatc
      961 gaaccgctgc acgcgcacgc cttgacgacg atggtcgcgg acgatgctgc gctcgaggcg
     1021 tgggtcgatc gaccgatcga gcaggtccgc agcgttcccg gtcccgcgct ctatcgccac
     1081 cgcgacgagg cggcgctgga ccgcctccgc gaagaactgc acgacgcact gcgcggccgc
     1141 gaggttgccg ccggtttcgt gcacggcgac ctctggctgg gcaacgtgct cgtcgatccg
     1201 gacgaacacg aagtgtgcgg ggtgatcgat tgggactttg cacgcgaacc ggtcccggtg
     1261 cagacggacg tcatgcacct gatcctcgcg acccgctcgg cgcgcgagaa aatggagctg
     1321 ggcgacgtcg tcgtcaggct tctcgcaacc gacgcgtggg aagactacga gttggacact
     1381 ttggcacgta cgggtgggct tggtgcatgt gatgttcctc cacgcacctt gctcttgttg
     1441 acctggttgg gacatgtgag cgggctaccg gacaagagcg accggtacag gaagagcttg
     1501 gtatggcgag cacgcaacac cgagcaagtg ctggaacgac tctgacggtc ctcgacgcgc
     1561 cgcttgccga gccgatccgc gagccggggc cggccgtagc gccggtcgaa cggcgcgcgc
     1621 cgtggatctt cccggtcgtc atgctcaccg ccgcgctcgc gtgctggatc gtggcgctgc
     1681 gcaacatcga tccccgcgac atcggcgact acggcctcat ctccgcgttc ccaccggcga
     1741 tgtatctcgc ggtcgcaata ctcacgctga gcttctgcgt cgtgctcaac acgcgagtac
     1801 gcgacgagct cctcggcgcg cacgtcgtgc tgctcgtcct catcatccac gcgaccccgt
     1861 cgatcaccta cggaacgctg cggtacgcgt gggcgtggaa gcacctcggc atcgtcgact
     1921 acttccaccg gaaccatgcg ttgtggcccg agtcaccgac actgaccgtg taccacaact
     1981 ggcccgggtt cttcacgttc gtcacgcatc tcaccgaagc gtcgggcctg gcgagcgcgg
     2041 gttccttcgc ggcatgggca ccgccattct tcgaactgct cttcgtcttg gcggtggcgg
     2101 cggtcatctc ggcgctcacg agcgaccgcc gcgtcatcgc gctcggcgtg tggctgttcg
     2161 tgctcgcgaa ctgggtcggc caggactact tcgcgccgca ggcgttcgcg tacttcttgt
     2221 acctcgtgct gttcgcgatc ctgctgcgtt ggttccgcaa cgagccggca ccgaagtatt
     2281 tcaaggggct tcggcatcga ctccagcggc gcgtgggccg ggcgctcgtc gaggacccgc
     2341 cgcacccggt gacggtcccg gaggctcagc agcgcacccc gttgctgttc ttcgtcgtgc
     2401 tcatgatgat cgcgatcgcg accagccacc cgctgacacc gttcgtcgcc atcgcgggcc
     2461 tgttggcgtt ggccgtcacc cggacgatca gcgtgcgggt cctgccgctc gtcatggcga
     2521 caatgacact cgcgtggttg tgcaccggcg cgtggccgta cgtcagcccg aacgcgcgcg
     2581 cgctcttcag cgagatcggc gcgctcggcg ccaacgtcga ctccaacctc cgcaacacat
     2641 cggtggtctc cgacgcgcag caactcgtct ccgcggccgg gaggtacgtc gtcgtgtcca
     2701 tggttgccct cgccgcgctc ggcgtgttgc gccggctgcg ggtcggtcgc ttcgagggcg
     2761 ccgccatctc gctcatggcc gcgccgatcg cggtcgcgat cgccggctcg tacggcggtg
     2821 aagtgatctt tcgcgtgttc ctgttcgcgt tgccgtttgc cgcgtacctc gcggcgcgcg
     2881 cattcatgtc gtccgacgac ggcgggcgcg gcatcctcac gacactggcc ctgatcgtgg
     2941 tcagcggtgt cctgctcgcc ggatcgttcc tcgcgtacta cggcaaggag cagtggacgc
     3001 atttctcaac cgacgaagtg cgcgccgcgc aagcagtgtt cgacaatgca cccgacgact
     3061 cgttgctgat cgcgttcgac cggtcgatgc cgtaccagtt cgcgaactac gaccgcttca
     3121 cctacgtgtg gatcctcgag gagccgagct cgtcggtgcg ccggatcctg aacgacccgg
     3181 ttggctcgct cgaggactgg atggagcaga aggaataccg gcggtcctac ttgctgatct
     3241 cgcgggcaca gagcgaggag accgccgccc tcggcgtact ccccgcgggc acgctcgaca
     3301 ccgtccgcga ccggttgaag gcgtcgggcg tcttcgacat cgtctacgac ggcccggacg
     3361 cgacggtgtt ctccctcgac ccgcacgcgg tcacgccgtg aaccggcgac tcgtgtgcgc
     3421 agtgtgggcg ctcgccgcgg tcgcggtgac cgggctcgag gcgccgcgcg cgatcgcgtt
     3481 cccggtcatg ttcgggttct tgtgcttcgt cccgggcttc gcactggtca gccggctgcg
     3541 gctgcagtcg ctcggcatcg aggtcgcgat cgcgttcggc gtcagcatgg cgctcgccgc
     3601 gatcgtcacc gaggtcctca tgtacgccga gttgtacagc gcgctcccgg cgacactgac
     3661 gctcgcgttg atcacgctca ccgcgtgcgc gtggccagtg tcgcgaacgc gggccagtca
     3721 gttgacctga cgttcgcgcc cttcccagta cggcgcgcgc agcttgaact tctgcagctt
     3781 gccggtggcg gtgcgggcga ggacatcacg gaactcgatg cgcgtcgggc acttgaagtg
     3841 cgtgagccgg tcgcggcagt gcgcgatcaa ctcgtcctcc gtcgcagaca caccgggtgc
     3901 cagcaccacg agcgcgatca cggtctcgcc ccacttctcg tcgggcacgc cgatgacgca
     3961 cacctcggcg acggccggat gtgagaacac aacgtcttcg acctcgatcg acgagacgtt
     4021 ctcaccaccc gagatgatga cgtccttctt gcggtccgcg atcgacaggt acgactcgtc
     4081 gtcgatgaga ccaccgtcgc cggtgcggaa ccagccgtcg cgaatcgcct tcgcggtctc
     4141 gtcgggctgt tcccagtagc cctccatgac gtggttggtg cgggcgagca cttcgccctc
     4201 ggcgtcgacc gcgaggcgga cacccagcgc gggcgcgccg gcgcggccga gccgggccgc
     4261 acggtcggcg ggactcagcg cgtcgtactc ctcgcggccg cggttcatcg tcagcagcgg
     4321 cgtggtctcc gtgaggccgt agatctggat gaac
//
LOCUS       DASUGA010000359         2611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_15822, whole genome shotgun sequence.
ACCESSION   DASUGA010000359 DASUGA010000000
VERSION     DASUGA010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15822"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VFX21_07130"
     CDS             <1..958
                     /locus_tag="VFX21_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX5095765.1"
                     /translation="LGLHNDLQNEHSIVGNNVGLLLMINYGTEQQKAEWIDDLAEGRR
                     GFAFGITEPAHGSDATHMETTAVRDGDEWVINGEKTFNTGMHRAQYDMVMARTSGEAG
                     DGNGITAFLTPTNSPGFEIVEFLWTFNMPTDHAHVRLTDVRVPDSAIFGGEGTGLQVT
                     QHFFNENRIRQAASSLGAAQFCIDQAVEYAKVRAPFGKPLATNQGIQFPLVELQTQCE
                     MLRALIHKTAWSMDKDGPFSVSDKVSMCNYWANRLCCEAADRAMQVHGGLGYSRHMPF
                     EHIYRHHRRYRITEGSEEIQMRRIAGYMFGFMKQRAPKGVTA"
     gene            955..>2611
                     /locus_tag="VFX21_07135"
     CDS             955..>2611
                     /locus_tag="VFX21_07135"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5095766.1"
                     /translation="MSDLPRDVTCDFTSSPFVGAIEPIAASDDEIRAALAEAEIPPLL
                     PALAYATGDMSLLREHLRPDPLLFGAMPQGGLHESQQAEVRELAFDALVKFRDGGCVS
                     APCPSDDDLLRIMEYAVGGAAMGAYLPLLEEELSYRGEDRRAPGFVLSDVAPGRTFRV
                     AIIGAGMSGLLAAHRLDQAGVEFVIFEKNGDVGGTWLENTYPGCRVDNPNHNYSYAFA
                     QRHDWPLHFSTQDVLLDYFRRCADAFDLRENIRFNTEVASAEWSDKDGNWTVTVRDGD
                     GLESSDVYDAVISAVGQLNRPLYPDIEGAGSFAGPTFHSARWDHSVDLHGKRVAVIGT
                     GASAVQFIPEIAPDPEHLYVYQRTPPWLGPTPDYHEPVSDGQRWLYAHVPSYSEWNRF
                     CIFWKMGDGVLDGVRVDPDFEPKGAAVSAANDMARMVMTGYLQMEFADRPDLLERVIP
                     TYPIGGKRLLRDNGVWARALKRDNVSLETTPIARITPSGIELADGTVHDADVLIYGTG
                     FQASKFLTPMQVRGRGGLDLHEQWGGDEARAYLGVTVPGFPNLF"
BASE COUNT          442 a          863 c          892 g          414 t
ORIGIN      
        1 gctcggcctg cacaacgact tgcagaacga gcactcgatc gtgggcaaca acgtcggctt
       61 gttgctgatg atcaactacg gcaccgaaca gcagaaggcg gagtggatcg acgacctcgc
      121 ggaagggcgg cgcggcttcg cgttcggcat caccgaaccc gcgcacggct ccgacgcgac
      181 acacatggag accaccgcgg tgcgcgacgg cgacgagtgg gtcatcaacg gcgagaagac
      241 attcaacacc ggcatgcacc gcgcccagta cgacatggtg atggcgcgca cgagtggcga
      301 ggcgggtgac ggcaacggca tcaccgcgtt cctcacgccg acgaacagtc ccggcttcga
      361 gatcgtcgag ttcctctgga cgttcaacat gccgaccgac cacgcgcatg tgcggctcac
      421 cgacgtgcgc gtccccgatt cggcgatctt tggcggtgag ggcaccgggc tccaggtgac
      481 gcagcacttc ttcaacgaga accgcatccg ccaggccgcg tcgagcctcg gtgccgcgca
      541 gttctgcatc gaccaggcgg tcgagtacgc gaaggtacgg gcgccgttcg ggaagccgct
      601 cgcgaccaac cagggcatcc agttcccgtt ggtcgagttg cagacgcagt gcgagatgct
      661 gcgggcgctg atccacaaga ccgcgtggtc gatggacaag gacggcccgt tctccgtctc
      721 cgacaaggtg tcgatgtgca actactgggc caaccggctg tgctgcgaag ccgcggaccg
      781 ggcgatgcag gtgcacggtg gtctcgggta ctcacggcac atgcccttcg agcacatcta
      841 tcgtcaccac cgccggtacc ggatcaccga aggcagtgag gagatccaga tgcggcgcat
      901 cgcggggtac atgttcggtt tcatgaagca gcgcgccccc aagggagtga cggcatgagt
      961 gacctgccgc gtgatgtgac gtgtgacttc acgtcgagcc cgttcgtcgg tgcgatcgag
     1021 ccgatcgccg cgagcgacga cgagatccgc gccgcgctcg ccgaggccga gatcccgccg
     1081 ttgctgcccg cgctcgcgta cgcgacgggc gacatgtcgc tgctgcgcga gcacctgcgg
     1141 cccgacccgt tgctgttcgg cgcgatgccg caaggcgggc tgcacgaatc ccagcaggcc
     1201 gaggtgcgcg agctcgcgtt cgacgcgctg gtcaagttcc gcgacggcgg ctgcgtctcg
     1261 gcgccatgcc cgagcgacga cgacctgctc cgcatcatgg agtacgcggt tggaggggcg
     1321 gcgatgggcg cgtacttgcc gctgctcgaa gaggagttgt cgtaccgcgg cgaggaccgg
     1381 cgcgcgcccg gcttcgtcct gtccgacgtg gccccgggcc gcacgttccg ggtcgcgatc
     1441 atcggcgcgg gcatgtcggg tctgctggcc gcgcaccggc tcgaccaggc cggcgtcgag
     1501 ttcgtcatct tcgagaagaa cggtgacgtc ggcggtacgt ggctcgagaa cacgtatccg
     1561 ggctgccggg tcgacaaccc gaaccacaac tacagctatg cattcgcaca gcggcacgac
     1621 tggccgttgc acttctcgac ccaagacgtc ctgctcgact acttccggcg ctgcgcagac
     1681 gcgttcgacc tgcgcgagaa catccggttc aacaccgaag tcgcgagcgc ggagtggtcc
     1741 gacaaggacg gcaactggac cgtgaccgtg cgtgacggcg acgggctcga gtcgtccgat
     1801 gtgtacgacg cggtaatcag cgcggtcggg cagctcaacc ggccgctgta tcccgacatc
     1861 gaaggtgccg gctcgttcgc gggtccgacc tttcactccg cgcgctggga tcattcggtc
     1921 gacttgcatg gcaagcgcgt cgcggtgatc ggcacgggcg cgagcgcggt gcagttcatc
     1981 cccgagatcg cgcccgatcc ggagcatctg tacgtgtacc agcgcacgcc accgtggctc
     2041 ggcccgacgc ccgactacca cgagcccgtc tccgacgggc agcggtggct gtacgcgcac
     2101 gtgccgtcgt acagcgaatg gaaccggttc tgcatcttct ggaagatggg cgacggcgtg
     2161 ctcgacggtg tgcgcgtcga ccccgacttc gagccgaagg gcgccgcggt cagcgccgcc
     2221 aacgatatgg cccgcatggt catgaccggg tacctgcaga tggagttcgc ggaccggccc
     2281 gacctgctcg agcgcgtcat cccgacgtac ccgatcggcg gcaagcggct gttgcgtgac
     2341 aacggcgtgt gggcgcgcgc gttgaagcgc gacaacgtgt cgctggagac gacgccgatc
     2401 gcgcgcatca cgccttcggg catcgagctc gccgacggca ccgtgcacga cgccgacgtg
     2461 ttgatctacg gcaccgggtt ccaggcgtcg aagttcctta cgccgatgca ggtgcgcggc
     2521 cgcggcggcc tcgatctcca cgagcagtgg ggcggcgacg aagcgcgtgc gtacctcggc
     2581 gtgacggtgc ccggtttccc gaacctgttc t
//
LOCUS       DASUGA010000360         2216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_446100, whole genome shotgun sequence.
ACCESSION   DASUGA010000360 DASUGA010000000
VERSION     DASUGA010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446100"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFX21_07140"
     CDS             <1..274
                     /locus_tag="VFX21_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095767.1"
                     /translation="LRLQLRYEYLQPETDEDPEPWEPDDDDLARIDDELRRAVDQMWE
                     EEEWRGVNEPDACSKCRFRSVCRDSATPGEAQWPVLSEAEEDPPRL"
     gene            285..1775
                     /locus_tag="VFX21_07145"
     CDS             285..1775
                     /locus_tag="VFX21_07145"
                     /inference="COORDINATES: protein motif:HMM:NF036848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEX5095768.1"
                     /translation="MTDPRAPVIVGVGQTSQRVPASEARAPLDLLADAARLAESDSGA
                     AMLGKVDIVAVVAIGSWKYNDPGAFLARQLGITPRATAVSTVGGNSPQLLVDEFATRI
                     QKNECDVVLIGGAESMHTRWRARREPKHELTWETGDDAPCEWIIGDDRPGSSQYEMAH
                     LAIAPTVVYPLFETALRAAAGRSVEEHQVHVSEMWARFSEVAAGNPDAWSRTALTPEE
                     IRTVSPDNRMVCFPYPKRMCANIDVDQGAAVLLCSYEAARAAGVPDDRIVFLHAAAEA
                     HDHYFVTERWSLAESPAIGAIGRAAFGATGLDVDDVARFDLYSCFPSAVQIGRAALGI
                     APADARPLTVTGGLGFAGGPVNNYPTHGIARMVQALRTDPGSIGCTTALGWYVTKHAL
                     ALWSTTPSDQPFQLHHPQAEVDALPRREPAGLVADECTVEATSVSVERDGTPTLGIVS
                     GLLDDGRRVIANARAVDTLTAMTTEPWEGRRVKIDNDGTTNAVAEG"
     gene            1808..2152
                     /locus_tag="VFX21_07150"
     CDS             1808..2152
                     /locus_tag="VFX21_07150"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEX5095769.1"
                     /translation="MTRRCARCGQGHLFTKWFKMVPNCPRCGLHFEREQGYWTGAIAV
                     NTIVIGGLFALIFVGTLIATAPDIPIVPLLLVVVPLMAIGPLVAYPFSKTIWVAIDLA
                     FLQPLGINFDGS"
BASE COUNT          374 a          746 c          745 g          351 t
ORIGIN      
        1 actccggctg cagctccgct acgagtactt gcaaccggag accgacgagg accccgaacc
       61 gtgggagccc gacgacgacg atctcgcccg catcgacgac gagttgcggc gcgccgtgga
      121 ccaaatgtgg gaagaggagg agtggcgagg cgtgaacgag ccggacgcgt gcagcaagtg
      181 ccgcttccgc tcggtctgcc gcgacagcgc gacgcccggc gaagcgcaat ggcccgtgct
      241 ctccgaggcg gaggaagatc cgccgcgctt gtagggtcgg cgttatgacc gatccccggg
      301 cgcccgtgat cgtgggcgtc ggccaaacgt cgcaacgcgt ccccgcaagc gaggcgcgcg
      361 cacccctcga cttgctcgcc gatgccgccc ggctcgcgga gtccgacagc ggtgcggcca
      421 tgctcggcaa ggtcgacatc gtcgcggtgg tcgcgatcgg ttcgtggaag tacaacgatc
      481 cgggcgcgtt ccttgcgcgc caactcggca tcacgccgcg cgcgaccgca gtgtcgacgg
      541 tcggcgggaa cagtccgcaa ctgctcgtcg acgaattcgc gacgcgcatt cagaaaaacg
      601 aatgcgatgt cgtgttgatc ggcggggccg agagcatgca cacccgttgg cgggcccggc
      661 gcgaaccgaa acacgaactc acgtgggaga cgggcgacga cgcaccgtgc gagtggatca
      721 tcggcgacga ccggcccggt tcgagccaat acgagatggc gcacctcgcc atcgcgccga
      781 cggtggtcta tccgctgttc gagaccgcgt tgcgcgccgc ggccggccgc tcggtcgaag
      841 aacatcaagt ccacgtgagc gagatgtggg cacgcttttc cgaagtcgcg gctggcaacc
      901 cggacgcgtg gtcgcgtacc gcgctgacgc ccgaagagat ccggacggtc tcaccggaca
      961 accgcatggt ttgcttcccg tacccgaagc gcatgtgcgc caacatcgac gtcgaccagg
     1021 gcgcggcggt gctcctctgc tcgtacgaag cggcgcgcgc cgcgggcgtc cccgacgacc
     1081 gcatcgtgtt cctgcacgcg gcggccgaag cgcacgatca ctacttcgtg accgagcgct
     1141 ggtcgctcgc cgagtcgccg gcgatcgggg cgatcgggcg ggccgcgttc ggcgcgaccg
     1201 gtctcgacgt cgacgatgtt gcgcgcttcg acctctactc gtgctttccc tcggccgtgc
     1261 agatcggccg cgccgccctc ggcattgcac cggcggatgc ccggccgctg accgtcaccg
     1321 gcgggctcgg gttcgcgggc ggaccggtca acaactatcc gacgcacggc atcgcacgca
     1381 tggtgcaggc gttgcggacc gatccgggaa gcatcggctg caccactgcg ctcggctggt
     1441 acgtcaccaa gcacgcgctc gcgctgtggt cgacgacacc atcggaccaa ccgtttcagt
     1501 tgcaccatcc gcaggccgag gtcgacgcgt tgcctcgccg cgaaccggcc ggactggtcg
     1561 ccgacgagtg cacggtcgag gccacatcgg tgtcggtcga gcgcgatggc acgccgacgc
     1621 tcgggatcgt gtccggactc ctcgatgacg gccgccgcgt gattgccaac gcgcgcgcgg
     1681 tcgataccct gactgcgatg accacggagc cgtgggaagg ccggcgcgtg aaaatcgaca
     1741 acgacggaac cacgaacgcg gtcgccgagg gatgaactcg agccgtactc ggaagctgtg
     1801 gcgcgggttg actcgccgct gcgcgcgctg cgggcaaggc cacttgttca cgaagtggtt
     1861 caagatggtc cccaactgtc cgcgctgcgg actgcatttc gaacgggaac aggggtattg
     1921 gaccggcgcg atcgcggtga acacgatcgt gatcggcggc ctgttcgcct tgatcttcgt
     1981 cggaactctg atcgcgaccg cgcccgacat cccgatcgtg ccgctgctgc tcgtcgtcgt
     2041 cccgctgatg gcgatcggcc cgctggtcgc ctatccgttc tcgaaaacga tttgggtcgc
     2101 gatcgacctc gccttcctcc aacccttggg catcaacttc gacggctctt agaatccatg
     2161 cgccaaatga cggcgaatac gccgtgagat ggcacatgga ttcattctga agccat
//
LOCUS       DASUGA010000361         3436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1389766, whole genome shotgun sequence.
ACCESSION   DASUGA010000361 DASUGA010000000
VERSION     DASUGA010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389766"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFX21_07155"
     CDS             <1..807
                     /locus_tag="VFX21_07155"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEX5095770.1"
                     /translation="RYRPAVVLGFGGYASLPCVVAARVLRVPVVVHEQDAAPGLANRI
                     GVKLGARAAVSLPGTPLTDATLTGNPVRAAFATLERAPQHDPAVVAVFGGAQGARTIN
                     RAALGCYDRWRARADIAVHHVCGERNLEESERVLAAARRPGDELQYELVGYEQDMPGL
                     YARASLAVCRAGAGTIAEVAAAGMPAIFVPLPNAPSDHQMRNAQTLERIGAAVVLRDE
                     ECDPRRLDAIVSELMRAPDRLERMSKAAKTVSRLDAAARVADLAEEHARG"
     gene            800..2185
                     /gene="murC"
                     /locus_tag="VFX21_07160"
     CDS             800..2185
                     /gene="murC"
                     /locus_tag="VFX21_07160"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011691431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX5095771.1"
                     /translation="MADALDLSTPKQVHIVGVAGAGMSAIALLLARMGHTVSGSDIKD
                     AAVLERLAAAGVAVQVGNRAENVPSTADAVVYSTAVPRNNPELVAAEKLGIPVLHRAA
                     ALAALSAIRRTVAVAGSHGKTTTASMLALVLRGAGLAPSFVIGGEVNEVGTNAAYGDG
                     DLFVVEADESDGTFLHLQTHAALVTNVEPDHLDYYGGFDGLVAAFERFVAGATGPVVC
                     SADDPVSARIAKSRPGVRTFGTARDAHYRIDDEHGDANGCRFTLVVGGETRGVIAVPV
                     GVKAATNATAALAVALELGVELDAASRALAGFGGVARRFQVRGERRGVTFIDDYAHLP
                     SEVAAAIATAKQGSGKRVIAVFQPHRYSRTASIGRDFADAFTAADMVVLTDVYPAGEQ
                     PIPGVSGRIVLEAVLDAHPGLPVAYLPRRADLLVVPDRYARPGDVVLTLGAGDLTALP
                     DVWLGNEPLTE"
     gene            2208..2975
                     /locus_tag="VFX21_07165"
     CDS             2208..2975
                     /locus_tag="VFX21_07165"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX5095772.1"
                     /translation="MTTTPTPTLRIDPRIRERLIAVRRQAGRRRLRWLIVALSLIAAL
                     GLSYLVVTSPILDVDHVRVGGAQHVTAEQVRAAAHIGSDDALLFVNTGAIARRVEEAL
                     PWVERATVERAFPGTVRITVHEYAPTAFVRAGNALVLLAADGRAIARVATAPAGAVEV
                     RGIRRAPNTGEYLAPMEASNIVPRLPRALAERVRAVDVGGGALSLQLAAGGTIRLGNA
                     NELDAKAQAALAVLAHLADASFNYIDVSTPQRPVSHQ"
     gene            3165..>3436
                     /locus_tag="VFX21_07170"
     CDS             3165..>3436
                     /locus_tag="VFX21_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018500051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX5095773.1"
                     /translation="MGAPQHYLAVIKVVGVGGGGCNAVNRMIDAGLKGVEFIAINTDA
                     QSLLLSDADVKLDIGRQLTRGLGAGSDPEVGRQAAEEHRQEIEEVL"
BASE COUNT          534 a         1171 c         1175 g          556 t
ORIGIN      
        1 cggtatcggc ctgcggtcgt gctcggattc gggggatatg cgtcgttgcc gtgcgtcgtc
       61 gcggcgcgcg tgctccgtgt cccggtcgtc gtgcacgaac aagatgctgc gcccggtctc
      121 gcgaaccgca ttggtgtcaa gctgggtgcg cgagccgcgg tttcgctgcc cggcacgcca
      181 ctgaccgacg cgacgctgac cggaaatccg gtgcgggccg ccttcgcgac gctcgaacgg
      241 gcaccgcaac acgatccggc agttgtggcg gtgttcggcg gtgcccaggg tgcgcgcacg
      301 atcaaccgcg ccgcgctcgg ttgctacgac cggtggcgag cgcgcgccga tatcgccgtc
      361 catcacgttt gcggcgaacg caacctcgag gagagcgagc gtgtgctcgc ggccgcgcgc
      421 cgtcccggcg acgagctgca gtacgaactc gtcggctacg aacaagacat gccggggctc
      481 tacgcccgcg catcgctcgc ggtgtgtcgc gcgggtgccg gcacgatcgc ggaagtcgcg
      541 gcggccggaa tgccggcgat cttcgtgccg ctgccgaacg cgccgagcga ccatcagatg
      601 cggaacgcgc agacactcga acgcatcggc gcggccgttg tgctgcgtga cgaggaatgt
      661 gatccgcgcc ggctcgacgc gatcgtgtcg gagctgatgc gggcgcccga tcggctcgaa
      721 cggatgagca aggccgcaaa gacggtcagc cggctcgacg cggcggcacg agtcgccgat
      781 cttgccgagg agcacgctcg tggctgacgc gctcgacctt tcgactccca agcaggtgca
      841 catcgtcggc gtcgcgggtg ccggcatgag tgcgatcgca ttgctgctcg cgcgcatggg
      901 tcacacggtc agcggatcgg acatcaaaga cgcggcggtg ctcgagcggc tcgcggcggc
      961 gggcgtcgcg gtgcaggtcg gcaaccgcgc cgagaacgtg ccctcgaccg ccgacgcggt
     1021 cgtctattcg acggcggttc cacgtaacaa ccccgaactc gtcgccgccg agaagctcgg
     1081 catcccggtg ctgcaccggg cggcggccct cgctgcgctc agcgccatcc ggcgcacggt
     1141 cgcggtggcg ggatcgcacg gcaagaccac gaccgcatcg atgctcgcgt tggtgctgcg
     1201 cggcgccggc ttggcgccca gcttcgtgat cggcggcgaa gtgaacgagg tcggcaccaa
     1261 tgccgcgtac ggcgacggcg acttgttcgt ggtggaggct gacgagagcg acgggacgtt
     1321 cctgcatctg cagacgcacg ctgcgctcgt cacgaatgtg gaacccgacc acctcgacta
     1381 ctacggcggg ttcgacgggc tcgtggccgc gttcgaacgg ttcgtcgcgg gcgcgaccgg
     1441 gccggtcgtc tgttcggccg acgatcctgt gtccgcacgc attgcgaaat cgcggccggg
     1501 ggtccggacc ttcggtaccg cgcgcgatgc gcactaccgc atcgatgacg agcacggtga
     1561 cgcgaacggt tgccggttca cgctcgtcgt cgggggcgaa acgcgcggcg tgatcgcggt
     1621 tccggtcggc gtcaaggccg cgaccaacgc gaccgcggcg ctggcggttg cgctcgaact
     1681 cggtgtcgaa ctggacgcgg cgagccgggc cctggccggc ttcggcgggg ttgcccgccg
     1741 tttccaggtc cggggcgaac gccgcggtgt cacgttcatc gacgattacg cccaccttcc
     1801 cagcgaagtc gcggccgcaa tcgcgaccgc caaacaagga tccgggaagc gcgtgatcgc
     1861 ggtcttccag ccccaccgct acagccgcac ggccagcatc ggccgcgact tcgccgacgc
     1921 gttcaccgcc gccgacatgg tggtgctgac cgacgtctat cccgcggggg agcagccgat
     1981 ccccggcgtc tcgggccgga tcgtgctgga ggccgtcctc gacgcccatc ccggcctgcc
     2041 ggtcgcgtac ctgccgcggc gtgccgatct gctcgtcgtg cccgaccgct atgcccgccc
     2101 cggcgacgtt gttctcacgc tcggagccgg cgacctgacc gcactgcccg acgtctggct
     2161 cggcaacgag ccgttgaccg aatgaacgct cgcatcgagg tgcgaccatg acgacgacgc
     2221 ccacaccgac cctgcgcatc gaccctcgca ttcgcgaacg gctcatcgcg gtgcgccgcc
     2281 aggccggccg ccgccggctg cgctggctga tcgtcgcact gagtctgatc gcggcgctcg
     2341 gcctgagcta tctcgtcgtc acctcgccca tcctcgacgt cgaccatgtc cgcgttggtg
     2401 gtgcacagca cgtgaccgcc gaacaagtgc gtgcggccgc gcacatcggt tctgacgatg
     2461 cgttgctgtt cgtgaacacc ggcgccatcg cgcgccgcgt cgaggaagcg ttgccgtggg
     2521 tcgagcgtgc aacggtcgag cgcgcattcc cgggcaccgt tcgcatcacc gtgcacgaat
     2581 acgccccaac tgccttcgtg cgcgccggca acgcactggt gctgctcgct gccgacggcc
     2641 gcgccattgc gcgcgtcgcg actgcacccg caggcgccgt cgaagtgcgc ggtatccgtc
     2701 gcgcaccgaa caccggtgaa tacctcgcac cgatggaagc gtcgaacatt gtgccgaggt
     2761 tgccgcgcgc gttggcggag cgcgtgcgcg cagtcgacgt cggtgggggt gcgctttcgt
     2821 tgcagctcgc agccggagga acgattcgtc tgggcaacgc gaacgaactc gatgcgaaag
     2881 cgcaagcagc attggcagtc ctcgcgcatc tcgccgacgc gtcgttcaac tacatcgatg
     2941 tgtcgacgcc gcagcgaccg gtttcccatc aatgaccgtt cgcttccacg ttgattcagc
     3001 acgcgcaaac gattaaggtc tcgacccgaa gcacaggttg acataattgt caacctcaag
     3061 ttgagcttca gggttggggg actctccaga cgttgttcca ggtggcagcc tggtcgcggg
     3121 ggccttccct tgctcgtccc gatgaagacg aaaggttgcg catgctgggc gctccgcagc
     3181 actacctcgc agtgatcaag gtcgttggtg tcggcggcgg cggttgcaat gccgtcaacc
     3241 gcatgatcga cgccgggttg aaaggcgtcg agttcatcgc catcaacacc gatgcacagt
     3301 cgctgctgct gtccgacgcg gacgtgaagc tcgacatcgg tcgccagctc acgcgcggac
     3361 tcggcgccgg atccgacccc gaggtcggac gtcaggccgc cgaagaacac cgccaggaaa
     3421 tcgaagaagt gttgaa
//
LOCUS       DASUGA010000362         2714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1874721, whole genome shotgun sequence.
ACCESSION   DASUGA010000362 DASUGA010000000
VERSION     DASUGA010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1874721"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="VFX21_07175"
     CDS             complement(<1..1520)
                     /locus_tag="VFX21_07175"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX5095774.1"
                     /translation="MTATETDLTAADVAWDVEPLLPAPGDAGLDDLLDRADAVADTLA
                     RARGNVGTMDAAALAEFMRGLGDVSELIGRAGSYAGLSFATDTTDPARGARMQRVEER
                     ATVISTKLVFFELEWAEVPDERVEALLADAQLAFARHHLRSALRYRPHLLTESEEVVL
                     TEKSVTGRSAWERLFEEQVSSIDVELDGEPATLEAALARLHAPSREVRADAAAAVTAG
                     LAPGLRTRAYILNTLLADKSTDDRLRSYPSWIASRNLANEASDESVQALVDAVQGRYA
                     IPQRWYALKAQLLGLDKLADYDRMASVADTEAQIGWAGAKDLVLDAYASFSGELADTA
                     QKFFDGGWIDAPARPGKRPGAFCAYTVPSHHPYVLLNWTARRRDVLTLAHELGHGLHA
                     YLAREQGIFHQTTPLTLAETASVFGETVTFGRLLGEVTDPQERLALLASNLEDQIATV
                     FRQIAMNRFENLMHTARREEGELSIDRLGELWAESQEAMLGDAVEVTDGYRSWWSY"
     gene            complement(1517..1921)
                     /locus_tag="VFX21_07180"
     CDS             complement(1517..1921)
                     /locus_tag="VFX21_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007620146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5095775.1"
                     /translation="MNADPVVAVGAIIVDDGRVLLVRRGRAPAAGKWSVPGGRVAAGE
                     QLRAAVAREVREETGLQVIVGDFAGWVERIDEQHHFVILDFFAGPVERARALVAGDDA
                     AEARWVPFAEVPRLDLVDGLLDFLRAVGSLPG"
     gene            complement(1918..2382)
                     /locus_tag="VFX21_07185"
     CDS             complement(1918..2382)
                     /locus_tag="VFX21_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5095776.1"
                     /translation="MEGARAATSADLPALVALATTLRAELRDQRGGALWEAHEARPEP
                     LEASLQVLLDRTDAGVFVGTIDDTIVGFGTVEIEELRTGGRLGVIGDLYVEPEARAVG
                     VGEAIAELLVAYCEQAKCIGIDAFALPGARAAKNFFERSGFTARALIMHKKL"
     gene            complement(2394..>2714)
                     /locus_tag="VFX21_07190"
     CDS             complement(2394..>2714)
                     /locus_tag="VFX21_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095777.1"
                     /translation="EPEPEPAVDTDVPAPPATPPAQHTTPYVPEPAPAPTVARSVPAN
                     GAPKSADLPIPGYDELSASQVVERLNGLAPRELDAVHAYETANRQRRTILGKIEQLAA
                     SPGV"
BASE COUNT          409 a          956 c          915 g          434 t
ORIGIN      
        1 atgtacgacc accacgaccg gtagccgtcg gtgacttcga ccgcgtcgcc cagcattgct
       61 tcttgcgact cggcccacag ttcgccgagc cggtcgatcg acagctctcc ttcctcgcgg
      121 cgcgcagtgt gcatcaggtt ctcgaaccgg ttcatcgcga tctggcggaa caccgtggcg
      181 atctgatctt cgaggttcga cgcgagcaac gccagccgct cttggggatc ggtgacttcg
      241 ccgagcaacc gtccgaacgt gacggtctcg ccgaacaccg acgcagtctc ggcgagggtg
      301 agcggcgtcg tctggtggaa gatcccctgc tcgcgcgcga ggtacgcgtg gagtccatgc
      361 ccgagttcgt gagcgagggt caacacgtcg cgtcggcgcg ccgtccagtt caacaacacg
      421 tacgggtggt gggacggcac ggtgtacgca caaaacgcgc cgggccgctt gcccggccgt
      481 gccggcgcgt cgatccagcc gccgtcgaaa aacttctgcg cggtgtcggc caactcgccc
      541 gagaacgacg cataggcatc gagcacgagg tccttcgcgc ccgcccaccc gatctgcgcc
      601 tcggtgtcgg ccaccgacgc catccggtcg tagtcggcaa gcttgtcgag cccgaggagc
      661 tgcgccttca acgcgtacca acgctgcgga atcgcgtacc ggccctggac tgcgtcgacg
      721 agcgcctgca cggactcgtc gctcgcctcg ttcgccaagt tccggctggc gatccagctt
      781 ggatacgacc gcagccggtc gtcggtcgac ttgtcggcga gcagcgtgtt caagatgtac
      841 gcccgcgtcc gcagcccggg cgcgagtccg gcggtcacgg ccgcggccgc gtcggctcgc
      901 acttcgcgtg acggagcgtg cagccgggcc agcgccgctt ccaacgtcgc cggttcgccg
      961 tcgagttcga cgtcgatcga cgacacctgc tcttcgaaca atcgctccca ggcgctgcgg
     1021 cccgttaccg atttctcggt caggacgact tcttcggact ccgtcagcag atgaggccgg
     1081 taacgcagcg ccgaccggag gtgatgacgc gcgaaggcga gctgcgcgtc ggcgagcagt
     1141 gcctcgacgc gctcgtcggg aacctcggcc cactccagct cgaagaacac gagcttggtg
     1201 ctgatgacgg tcgcgcgctc ctcgacgcgc tgcatccgag cgccgcgcgc cggatcggtt
     1261 gtgtcggtcg cgaacgacag acccgcgtag ctgccggcgc gcccgatgag ctcgctgacg
     1321 tcgcccagcc cgcgcatgaa ctccgcgagc gcggccgcgt ccatcgtgcc gacatttccg
     1381 cgtgctcgag cgagcgtgtc cgcgacggca tccgctcgat cgagcagatc atcgagcccc
     1441 gcgtcgccgg gcgccggcaa cagcggttcc acatcccacg caacgtcggc ggcggtgagg
     1501 tcggtttcgg tcgcggtcat cccgggaggc taccgaccgc acggagaaag tcgagcagcc
     1561 cgtcgacgag gtcgagccga gggacttccg cgaacggcac ccaccgcgct tcggccgcgt
     1621 cgtcgcccgc gacgagcgca cgagcacgtt cgaccggtcc ggcgaagaag tcgaggatga
     1681 cgaagtgatg ctgctcgtcg atgcgctcga cccagcccgc gaagtcgccg acgatcacct
     1741 gcagcccggt ctcttctcgt acctcgcgcg caaccgccgc gcggagctgc tcccccgctg
     1801 cgacacgtcc gcctggaacc gaccactttc ccgccgccgg tgcccggccc cgccgcacga
     1861 gcagcacgcg gccgtcgtcg acgatgatcg caccgaccgc aacgaccggg tcggcgttca
     1921 cagcttcttg tgcatgatca gggcgcgcgc cgtgaaccct gatcgttcga agaaattctt
     1981 ggcagcacgc gcgccgggca acgcgaacgc atcgatgccg atgcacttcg cctgctcgca
     2041 gtacgcgacg agtagctccg cgattgcctc accgacaccg acggcgcgtg cttcgggttc
     2101 gacgtagaga tcgccgatga cgccgagccg gccgccggtg cgcagttcct cgatctcgac
     2161 cgttccgaat ccaacgatcg tgtcgtcgat ggtgccgacg aacactcccg cgtcggtgcg
     2221 atcgagcaag acttgcaacg acgcttccaa cggttcgggc cgggcctcgt gggcttccca
     2281 cagcgcgcca ccgcgctggt cacgaagctc ggcgcgcaag gttgtcgcga gcgcgacgag
     2341 ggcgggcagg tctgcgctcg tcgcggcccg cgccccttcc accagcgatc cgttcagacg
     2401 cccggtgacg cggcgagctg ttcgattttg ccgaggatcg tgcgccgctg gcggttcgca
     2461 gtctcgtagg cgtgcaccgc gtcgagttcg cgcggcgcga ggccgttcag gcgttcgacg
     2521 acctgcgatg ccgagagctc gtcgtagccg ggaatcggaa ggtcggcgct cttcggcgcg
     2581 ccgttcgcgg ggacggagcg cgcgacggtc ggcgcgggcg cgggctcggg aacgtacggc
     2641 gtcgtgtgct gcgcgggcgg ggtcgccggc ggcgcgggta cgtccgtgtc gactgcgggt
     2701 tccggttcgg gttc
//
LOCUS       DASUGA010000363         2238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_83314, whole genome shotgun sequence.
ACCESSION   DASUGA010000363 DASUGA010000000
VERSION     DASUGA010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83314"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VFX21_07195"
     CDS             <1..788
                     /locus_tag="VFX21_07195"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HEX5095778.1"
                     /translation="GVLFERDAAIDTAIAFVCFCLAASGTYFINDALDAEADRRHPTK
                     CTRPVASGAISVQTAVIGGVVLLVAAVSLSFAARAQLALVVGGYLALTVAYSLWLKHE
                     AVLDLAAVAAGFVLRTIAGGVAVGVVISPWFLIVAGAGSLFMVTGKRHAELLELGDDA
                     ADHRPALQMYSHAYLGYVRAVASSVAVLAYCLWAFEKSGNTGDPMWFELSIVPFVIAI
                     LRYALLLEQGRGGAPEELVLSDKTLLAVGAVWAVLFGIAVHYG"
     gene            781..2154
                     /locus_tag="VFX21_07200"
     CDS             781..2154
                     /locus_tag="VFX21_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003998645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5095779.1"
                     /translation="MADVEAGTDELLTGWGRTRPTRATVAEPAHLDEIVARLSHAGPR
                     GVIARGLGRSYGDAAQNAGGAVLRLTEMHRMLALDLDKGTCTVEAGVSLDTLMRALLP
                     LGWFPMVVPGTRFVTVGGAIASDIHGKFRHGTFSDYVERIQLATPGRVLTCGPDLEPD
                     TFWATVGGMGLTGVITEATLQLQPVETALITSDSERVADVDDCMARMLERDDEYRYSV
                     AWIDCMARGRHLGRSVLSRGNHTRLDELTPSARADARVFAPRAVATAPKFVPNGLLNL
                     LSVRAFNELWFRKPQSSRRGHIESITSFFHPLDGVQGWNRIYGSRGFVQYQYVVPYGA
                     EDVVRGTLERLSATRCPSFLAVIKRFEHASGGLLGFPAPGWTLALDVPATPHGLDGLL
                     DGLDELVVEAGGRVYLTKDSRLRPELLDEMYPQLDRWRAIRAGLDPKHVMRSDMDRRL
                     HLSGEHS"
BASE COUNT          317 a          809 c          742 g          370 t
ORIGIN      
        1 ccggagtgct gttcgaacgc gacgccgcga tcgacaccgc gatcgcgttc gtctgcttct
       61 gtctcgcggc gagcggtacg tacttcatca acgacgcgct cgacgccgaa gccgatcgcc
      121 ggcacccaac caagtgcacg cgcccggtcg cgtcgggcgc gatctccgtg cagacggccg
      181 tcatcggcgg cgtcgtactc ctcgtcgccg cggtcagcct ctcgttcgcc gcccgcgcgc
      241 agctcgcgtt ggtcgtcggc ggatatctcg cgctcaccgt cgcgtacagc ctctggttga
      301 agcacgaggc cgtgctcgat ctcgcggcag tggcggccgg gttcgtgctg cgcacgatcg
      361 ccggtggtgt cgcggtcggt gtcgtgatct cgccgtggtt cttgatcgtc gcgggcgccg
      421 gttcgctgtt catggtcacc ggcaagcggc acgccgaact gctcgagctg ggtgacgacg
      481 cagccgatca ccggcccgcg ctccagatgt actcgcatgc gtacctcggc tacgtacgcg
      541 cggtcgcgtc gagcgtcgcg gtgctcgcgt actgcctgtg ggcgttcgag aagtcgggca
      601 acaccggcga ccccatgtgg ttcgagctct cgatcgtgcc gttcgtgatc gcgatcctgc
      661 ggtacgcact gctcctcgaa caagggcgcg gcggcgcacc cgaggagctc gtgctgtccg
      721 acaagacgtt gctggcggtc ggcgcggtat gggccgttct gttcggcatc gcagtccact
      781 atggctgacg tcgaggccgg taccgacgag ttgctgaccg gctggggccg cacccggccg
      841 acacgcgcaa ccgtcgccga acccgcgcac ctcgacgaga tcgtcgcccg cttgtcacac
      901 gcgggaccgc gcggtgtgat cgcgcgcggg ctcggtcgta gctacggcga cgccgcgcag
      961 aacgcagggg gcgcggtgct gcgtctcacg gagatgcacc gcatgctcgc gctcgacctc
     1021 gacaagggca cgtgcaccgt cgaagcgggc gtcagcctcg acacgctcat gcgggccctg
     1081 ctgccgttgg gctggttccc gatggtcgtg cccgggacgc gcttcgtcac cgtcggcggc
     1141 gcaattgcgt ccgacatcca cggcaagttc cgccacggca cgttctccga ctacgtcgag
     1201 cggatccagc tcgcgacgcc cggacgagtc ctcacgtgcg gtcccgacct cgaacccgac
     1261 acgttctggg ccaccgtcgg cggcatgggg ctcaccggcg tcatcaccga ggccacgctg
     1321 cagctccagc cggtcgagac cgctctcatc acctccgact ccgagcgcgt cgccgacgtc
     1381 gacgactgca tggcccgcat gctcgagcgc gacgacgaat accgctactc cgtcgcgtgg
     1441 atcgactgca tggcgcgcgg cagacatctc ggtcgctcgg tactctcgcg cggcaaccac
     1501 acgcgtctcg acgagctcac cccgtccgca cgcgcggacg cgcgcgtctt tgccccgcgc
     1561 gccgtcgcga ccgcgccgaa gttcgtaccc aacgggctgc tgaacctgct ctcggtgcgc
     1621 gcgttcaacg agttgtggtt tcgcaagccg cagagctcgc ggcgcgggca catcgagtcg
     1681 atcacgagct tcttccaccc cctcgacggc gtgcagggct ggaaccgcat ctacggctca
     1741 cggggcttcg tgcaatacca atacgtcgtg ccgtacggcg ccgaagacgt cgtacgcggg
     1801 acgctcgaac gactcagcgc gacgcggtgc ccgtcgttcc tcgcggtgat caaacgcttc
     1861 gagcacgcga gcggcgggct cctcgggttc ccggcaccgg gctggacgct cgcgctcgac
     1921 gtcccggcca ccccgcacgg cctcgacggg ctgctcgacg gcctcgacga gctcgtcgtc
     1981 gaggcgggcg gccgcgtgta cctgaccaag gactcccggt tgcggcccga gctgctcgac
     2041 gagatgtacc cgcagctcga ccggtggcgg gcgatccgcg ccgggctcga ccccaagcac
     2101 gtcatgcgct ccgacatgga ccgccgcctg catctctcgg gagaacattc atgaaggacg
     2161 cgctcggcgc cgtggaatcc attctcgtgc tcggcggcgg ctccgacatc gcgatcgcga
     2221 cgtgcgagaa gctcgcac
//
LOCUS       DASUGA010000364         3292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1657817, whole genome shotgun sequence.
ACCESSION   DASUGA010000364 DASUGA010000000
VERSION     DASUGA010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657817"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VFX21_07205"
     CDS             complement(<1..200)
                     /locus_tag="VFX21_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020461226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5095780.1"
                     /translation="MPDFDASDFFRGSDFVADPYPYFEYLRAQCPVQREPHHDVVMVT
                     GYEEAVAIYNDTARFSSCNSVTG"
     gene            complement(269..1099)
                     /locus_tag="VFX21_07210"
     CDS             complement(269..1099)
                     /locus_tag="VFX21_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5095781.1"
                     /translation="MDRIDDALAELGWLDMLATEPDDAIAIVFETLGALNATASVLDD
                     VVVTGLGLLPSPTLAAALPAFGTWVAPGPAALATARVTTATEIVAGGHPVPIARATSA
                     PLGGIDPTMKLFTVSIETTEILESWDDAVALGRRAIAHQIAGATRAMLDFARTHALER
                     VQFGRPVARFQAVRHKLAESLVAIEALDATLGAAAAEPGPLTAALAKAVAGQTARTVG
                     THCQQVLAGIGFTTDHAFHTYLRRTMLLDGLFGAADDVVVAIGHELLAGGSVPTLIEL
                     "
     gene            complement(1159..2310)
                     /locus_tag="VFX21_07215"
     CDS             complement(1159..2310)
                     /locus_tag="VFX21_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019927795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5095782.1"
                     /translation="MSFSAPNPDMTAYRAELHDWLDAHAGELAPPYAEPGTLDEQMAQ
                     MQRVKGLLFDAGWMRYGWPEHVGGLGGSPLLRTELGATLAARDIIDPGLFSLIEVLAP
                     TLIDYAPPALAAAVVPALLSGAEMWCQGFSEPETGSDLGGLRCRAVADGNERWIVNGQ
                     KVWTSLAQYADRCVLLTRTGDVESRHRGITAFFVDMDTPGITVQPIEMINGVYEFTEV
                     FFDDVIVPADRVLGEVNGGWAVAMSILPYERSSCFWQRIAYLYNRLGRLTAVAPADDR
                     TAETIGHAFVQLHAARARSLATQHRLAAGATLGAETSIDKVLIATAEQATYAAVQKVL
                     RTQIATGDDVGAEMWRNEYLYSRAATIYGGTAEVQRNIIARRLLDLGDE"
     gene            2369..>3292
                     /locus_tag="VFX21_07220"
     CDS             2369..>3292
                     /locus_tag="VFX21_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5095783.1"
                     /translation="MDTWHEERLLIDGELVAASGGKTYENVNPATEEVIGTSADASVA
                     DTERAIAAARRAFDKTEWSRDRDLRVRGLQQLHAALVKHADELRTILVQEVGAPLQST
                     FGPQLDGPTAVVEWYGEMLKTYEFVEDLGEREAFGLNNHRWTEKEAAGVVGAIAAYNY
                     PIQLALAKLAPALAAGCTVVLKGAPDTPWSTLALGQLIAEETDIPPGVVNVLASSDNA
                     VGALLTTHPDVDVVSFTGSTPVGRQIMAAASDTVKRVFLELGGKSAFVMLDDGDVNMA
                     AMFCAFAAVSHSGQGCAITSRLVVPRDRYDEA"
BASE COUNT          543 a         1097 c         1128 g          524 t
ORIGIN      
        1 ccggtcaccg agttgcaaga cgagaagcgc gcggtgtcgt tgtagatcgc gacggcttcc
       61 tcgtagccgg tgaccatcac gacgtcgtga tgcggctccc gctgcaccgg acactgggcc
      121 cgcaggtact cgaagtaggg gtacggatcc gcgacgaagt cgctgccccg aaagaagtcg
      181 ctcgcgtcga aatcaggcac accatctccc ggtgaatact tcgttcacac actaggagag
      241 cggcgtttcc gccgcaacgg agtgcaggtc agagctcgat cagcgtcggt accgacccgc
      301 cggcgagcag ctcgtggccg atcgcaacca cgacgtcgtc ggctgcgccg aacagcccgt
      361 cgagcaacat cgtgcgccgg agatacgtgt gaaacgcgtg gtcggtcgtg aagccgatgc
      421 cggcgaggac ctgctggcag tgcgtgccga ccgtgcgcgc cgtttgcccg gcgaccgctt
      481 ttgccagcgc agccgtgagc ggtcccggtt cggccgcggc cgcgcccagg gtcgcgtcga
      541 gggcttcgat cgcgacgagc gattccgcga gcttgtgccg gacggcttgg aaacgcgcaa
      601 ccggccgccc gaactgcacg cgttcgagcg cgtgtgtgcg cgcgaagtcg agcatggcgc
      661 gggtcgcgcc cgcgatctgg tgcgcgattg ctcgccgtcc gagtgcaacc gcgtcgtccc
      721 aggattcaag gatctcggtc gtttcgatcg aaacggtgaa cagcttcatc gttggatcga
      781 tgccgccgag cggcgcagac gtcgcgcggg cgatcggcac cgggtggccg ccggcgacga
      841 tctccgtcgc ggtcgtcacg cgtgcggttg caagtgcggc cggaccgggc gcaacccacg
      901 tgccgaacgc gggcagcgca gctgcaagcg ttgggctcgg gagcagaccc aaccccgtga
      961 cgacgacgtc gtcgaggacg gacgcggttg cgttcagggc accgagcgtc tcgaacacga
     1021 tggcgatcgc gtcgtccggt tcggtcgcga gcatgtcgag ccagccgagt tccgccagtg
     1081 cgtcgtcgat ccgatccacg ggggcagccg cgattgcgtc gcgcacggcg ccccgaagca
     1141 gctctcgttc ttcgggtgtc actcgtcccc caagtcgagg aggcggcggg cgatgatgtt
     1201 gcgttgcact tcggcggtgc cgccgtagat cgtcgcggcg cgggagtaca agtactcgtt
     1261 gcgccacatc tccgcgccga cgtcgtcgcc ggtcgcaatc tgcgtacgga gcactttctg
     1321 caccgccgca tacgtcgcct gctcggcggt cgcgatcagg accttgtcga tcgaggtctc
     1381 ggccccgagc gtcgcgcccg cagcgagccg gtgctgggtt gcgagcgagc gggcgcgcgc
     1441 cgcgtgcagc tgtacaaatg catgaccgat cgtctcggcg gtgcgatcgt cggccggcgc
     1501 gacagccgtg aggcgcccca accggttgta caggtacgcg atgcgctgcc agaagcacga
     1561 cgaacgctcg taggggagga tgctcatggc gacggcccac ccgccgttca cttcgccgag
     1621 cacacgatcg gccggcacga tgacgtcgtc gaagaacacc tcggtgaact cgtaaacgcc
     1681 gttgatcatc tcgatcggtt gcaccgtgat gcccggcgtg tccatgtcga cgaagaacgc
     1741 ggtgatcccg cggtggcgcg actccacgtc gcccgtgcgc gtgagcagca cgcagcgatc
     1801 ggcgtactgc gccaagctcg tccacacttt ctggccgttg acgatccagc gctcgttgcc
     1861 gtcggcgacg gcgcggcagc gcaggccgcc gagatcgctg ccggtctcgg gttcgctgaa
     1921 tccttggcac cacatctccg cgcccgacag caacgcgggc acgaccgcag ccgcgagtgc
     1981 gggcggcgcg tagtcgatca gtgtgggtgc gaggacttcg atcagggaga acaggccagg
     2041 gtcgatgatg tcgcgcgccg ccaacgtcgc gccgagttcg gtgcgcagca gcggtgagcc
     2101 gccgaggccg ccgacgtgtt cgggccagcc gtaccgcatc cacccggcgt caaacagcag
     2161 acccttgacg cgttgcattt gcgccatctg ctcgtcgagt gttccgggct ccgcgtacgg
     2221 cggcgccagt tcgccggcat gcgcgtcgag ccagtcgtgc agctcggcgc gatacgccgt
     2281 catgtcgggg ttgggcgcac taaaggacac ggtgagaacc ccgcatgcgt tttgaagaga
     2341 aagccgttct cgccgtagcg taaccgccgt ggacacatgg cacgaagagc gattgctgat
     2401 cgacggtgaa ctggtcgcgg cgagcggcgg caagacgtac gagaacgtca accccgcgac
     2461 cgaggaagtg atcggcacct ccgccgacgc gtcggttgcc gacaccgaac gcgcgatcgc
     2521 ggcggcgcgc cgcgccttcg acaagaccga atggtcgcgc gaccgcgacc tgcgtgtgcg
     2581 cggactccaa cagctgcacg ccgcgctcgt gaaacatgcc gacgaactcc gaacgattct
     2641 cgtgcaagag gtcggtgctc cgttgcagag cacgttcggg ccgcagctcg acggcccgac
     2701 cgccgtcgtc gagtggtacg gcgagatgct caagacgtac gagttcgtcg aggatctcgg
     2761 cgagcgcgaa gcgttcggcc tcaacaacca ccgctggacc gagaaggaag cggcgggagt
     2821 cgtcggcgcg atcgcggcgt acaactatcc gatccagctc gcgctggcga agctcgcccc
     2881 ggcgctggct gcaggctgca cggtggtgct caagggcgca cccgacacgc cgtggtcgac
     2941 gctcgcactc ggccagctca tcgccgaaga aacggacatc ccgcctggcg tcgtgaacgt
     3001 cctcgcgtcc tccgacaacg ccgttggcgc gctgctcacc acgcatcccg acgtcgacgt
     3061 cgtgtccttt accggatcca ccccggtcgg ccgtcagatc atggccgcgg ccagcgacac
     3121 cgtgaagcgc gtgttcctcg agttgggcgg caagtcggcg ttcgtcatgc tcgacgacgg
     3181 cgacgtgaac atggctgcga tgttttgcgc gttcgcggcg gtctcacact cgggccaagg
     3241 ttgcgcgatc acctcgcgct tggtcgtgcc gcgcgaccgc tacgacgaag cg
//
LOCUS       DASUGA010000365         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_112803, whole genome shotgun sequence.
ACCESSION   DASUGA010000365 DASUGA010000000
VERSION     DASUGA010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112803"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1430)
                     /locus_tag="VFX21_07225"
     CDS             complement(111..1430)
                     /locus_tag="VFX21_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX5095784.1"
                     /translation="MATTTNDRHDPPLVEIERAVQTRAKELALDVSGPRGESELRALI
                     DDEVRRWNDEFHRGVRVHELTHPQIVAERAFRNLARFGPLTPLLLDDDVWEIMINGPD
                     QIFVKRHREADGYHHEGFHDNEHVLRTLTRVLDESSQNHRKLDASEGLQDAQLDDGSR
                     IHIVHGDLSHGGNAMVNIRKFTGVPFRSLRELVERGMLDAFAARFLRAAVRANASIVF
                     AGAPGSGKTTMLSCCAAELDPALRVVVAEEVFEADVPLPNVASMQTRPARSDRPAIDL
                     RRLVAGFLRMAPDVAIVGEVRDREALPLLLTLSSGVTGFTTIHAGSARQALTRLRFVA
                     QLADEASNLPMSALNTLVSEAIDVVVHCRRRHHGPQVSEIIAVEDLIAGHDASNFTTT
                     ELFVRNTPDERLTWTGNVPVRLGKVFHTSGLRLADYLGERIPRREEP"
     gene            1554..>2009
                     /locus_tag="VFX21_07230"
     CDS             1554..>2009
                     /locus_tag="VFX21_07230"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEX5095785.1"
                     /translation="MLRRSTSIAAIAAVLLASCVLFVVDAPEAQAQEPAPHLVSRLTI
                     GDATIVEGSGGTKAGRKLALRLSQPLSFDLTVNYTTDGGTATAPSDYKDKDGKVTIKA
                     GKTIKFLSPKVIGDDVAEGTETFSITLTSATPLIPAIGSVFFAKRQGTVT"
BASE COUNT          345 a          676 c          652 g          336 t
ORIGIN      
        1 ccggcgttct actcgtgcct cgtctcgctc gagggctgga cgttgcggtc cgcgaacgcc
       61 gatcagcaga tggacgccag acgccgcgaa cagcgcgagc acgaggccgg tcatggctct
      121 tcccggcgcg gaatgcgctc accgaggtag tcagccagcc gcaaccccga cgtgtggaac
      181 accttgccca gccgaaccgg cacgttgccc gtccacgtca accgctcgtc cggtgtgttg
      241 cgaacgaaca gctcagtcgt cgtgaaattg gaggcgtcgt gccctgcgat gaggtcctcc
      301 acggcgatga tctcgctcac ctgtggaccg tgatgccggc gccggcaatg gacgacgacg
      361 tcgatcgcct cgctcacgag cgtgttgagt gcgctcatcg gcagattgct ggcctcgtcg
      421 gcgagctgag cgacgaaccg gagtcgggtg agcgcctgtc gcgccgaacc tgcgtgaatg
      481 gtcgtgaaac cggtgacgcc cgacgaaagc gtgagcagca atggcaacgc ctcgcggtcg
      541 cggacctcac cgacgatggc gacgtccgga gccatgcgga gaaacccggc gaccagtcgg
      601 cgcaggtcga tggccggtcg atccgaacga gccggccggg tctgcatgct cgcgacgttg
      661 ggcaagggaa cgtcggcctc gaacacctcc tcggccacaa cgacccggag cgcagggtcg
      721 agctccgcgg cgcagcacga cagcatcgtc gtcttaccgc ttcccggcgc cccggcgaac
      781 acgatcgacg cgtttgcacg caccgcggcc cggaggaacc gagccgcgaa cgcgtcgagc
      841 atcccgcgtt cgacgagctc gcggagtgat cggaagggca ccccggtgaa cttccgaatg
      901 ttcaccatgg cgttgccgcc gtgactgaga tcgccatgca cgatgtggat gcgcgaaccg
      961 tcgtccaact gcgcgtcttg cagcccttcg ctggcatcga gctttcggtg gttctgggaa
     1021 gactcgtcca gcacgcgtgt caaggtgcgc aggacgtgct cgttgtcgtg gaacccctcg
     1081 tggtggtagc cgtcggcctc gcgatgtcgc ttcacgaaga tctgatccgg gccgttgatc
     1141 atgatctccc acacgtcgtc gtcgagcagg agcggcgtga gcgggccgaa cctggccagg
     1201 ttccggaagg cgcgctcggc gacgatctgc ggatgcgtga gctcgtgcac gcgcacgccg
     1261 cgatggaact cgtcgttcca gcgcctcacc tcgtcgtcga tgagcgcgcg gagctccgac
     1321 tctccacgcg gaccgctcac atccagcgcg agctccttgg cgcgcgtctg cacggcacgc
     1381 tcgatctcga ccagtggggg gtcgtgccgg tcgtttgtgg tggtcgccat cgccggaagg
     1441 taaatcggcg acgtacccgt ccgcgccatg acatgtttca ccggaaccgg gtcccgtcgc
     1501 gggtcaacgg cgggccctag atcgtccgat acggccgcgg taccttccgt ctcatgttgc
     1561 gccgttcgac atccattgcc gccatcgctg cggtgctgct cgcgtcgtgt gtgctcttcg
     1621 tggtcgacgc acccgaggcg caggcgcagg agcccgcacc acaccttgtg tcgcgcctca
     1681 cgatcggcga cgccacgatc gtcgagggca gtggcggaac gaaggccggc cgcaagctgg
     1741 cattgcgcct gtcgcaaccg ctctcgttcg acctcacggt gaactacacg accgacggcg
     1801 gcaccgcgac cgcaccatcc gactacaagg acaaagacgg caaggtgacc atcaaggcgg
     1861 gcaagaccat caagttcttg tcgcccaagg tgatcggcga cgacgtcgcc gaaggtaccg
     1921 agacgttttc gatcacgctc acctccgcga caccactcat tcctgcaatc ggatccgtgt
     1981 tcttcgccaa gcggcaggga acggtcacg
//
LOCUS       DASUGA010000366         4436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_847128, whole genome shotgun sequence.
ACCESSION   DASUGA010000366 DASUGA010000000
VERSION     DASUGA010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847128"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VFX21_07235"
     CDS             complement(<1..795)
                     /locus_tag="VFX21_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 65 glycosyl hydrolase"
                     /protein_id="HEX5095786.1"
                     /translation="MTDEHEFGTDAWCIVERELHLDRLAHLESLFALSNGHIGLRGNL
                     DEGEPHAVPGTYLNGFFETVPLPYAEVGYGYPEDGQTLIDVTNGKIMRLLVDDEPFDV
                     RYGTLTHHERVLDMRHGLLDRRVQWTSPARQSVAIRSTRLVSFVQRAIVAIRYEVQAV
                     DAAARVVLQSSLVANEPVPTRTADPRAAAALDQPLVADFHTHHDVEVALGHHTRVSGL
                     RMAAALDHLVDGPDGTVTACESDPDLARVTVTTELQPGDTLTVVKLL"
     gene            complement(792..1544)
                     /locus_tag="VFX21_07240"
     CDS             complement(792..1544)
                     /locus_tag="VFX21_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009720866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HEX5095787.1"
                     /translation="MNASPRLPDGIRACLFDLDGVLTQTARVHAAAWKEMFDQYLSDR
                     ARRVGGDLRPFSLPDDYARYVDGKLREDGVRAFLASRNIDLPDGSADDPPSAETVAGL
                     ATRKNELLLEMIDAGGVDVYASSVRFVEAVRDARLPRAVVSASKNCRAILEAAHIDHL
                     FDARVDGVVAAQRNLRGKPAPDMFLAAAALLDVAPQHAAVFEDALAGVEAGRSGGFGC
                     IVGVDRVDQTAALRSHGADIVVSDLGELLEDP"
     gene            1833..2219
                     /locus_tag="VFX21_07245"
     CDS             1833..2219
                     /locus_tag="VFX21_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095788.1"
                     /translation="MNVRRGPHQHPSHARDRRRGYELWVTCADCGDVVVRAELCELHA
                     RAGDFTIVFPCSQCARRDVVPVTTEDVLELVAAGFVIRRQSAAAELREHRPCGEPFAW
                     DDLLAFHEDLASATDVVALLDQTRVR"
     gene            complement(2224..3075)
                     /locus_tag="VFX21_07250"
     CDS             complement(2224..3075)
                     /locus_tag="VFX21_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095789.1"
                     /translation="MLEPVIGQVYFSPECHEAYRTLGFGPSPGMFNNVAMPDGSAYFT
                     SRGSLMGQVEGEVVAAAFGVFKPEVVVDGVRAGWNLTDAPTIFAARRNGAVAQLVRIL
                     GPADANVDRAIALMTRAVDPLAPPGRPLFAGLRAQWDDPSDPWTRLFHLGDMLREYRG
                     DAHVAAWSSADVDAPEIGLLMEAYIGMPLRTYVRTRAWNDEELEAAVQRLNARGWLDG
                     ETLTETGRHEREEIECATDRALRPAIEALGDDVGELCALLRPWGEAVRAAGGYVGGPV
                     DLWPSRD"
     gene            complement(3467..>4436)
                     /locus_tag="VFX21_07255"
     CDS             complement(3467..>4436)
                     /locus_tag="VFX21_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095790.1"
                     /translation="GREVEALALADELAALDAEISARRPRATVGRELAKWERVTGEAR
                     TRLVECHEQAARVQPADLAEALRLRNEWRYAEQVHRQMRRRRTRRDVDELKQHYERFL
                     GRFGAATYEDLAVVGTGFGNTDADLAIREAATVVSMAEQHCIDLRAELETSDPAALAR
                     RRQDALARASVLLGSEADADVVTKLRRLQPVAAGAVTASDTDRATDRAVDELDRLAIE
                     VRALEVEQARLERSRRDLVARAPIDLPGVLQALLDADDERSASTMLPPLVVDHVFAGF
                     VPTARRRVFDALVAHSRVRQVILVDDDPELVTWAAACDAVVWSATQ"
BASE COUNT          650 a         1494 c         1593 g          699 t
ORIGIN      
        1 gagcagtttg accacggtca gcgtgtcgcc cggctgcagt tcggtggtga ccgtcacccg
       61 ggcgaggtcc ggatcgctct cgcacgcggt gacggtgccg tcgggtccgt cgacgaggtg
      121 atcgagcgcg gcggccatgc gtagaccgct gacgcgcgtg tggtggccga gcgcgacctc
      181 gacgtcatgg tgggtgtgga agtcggcgac gagcggctgg tcgagcgcgg cggcggcgcg
      241 cggatcggcc gttcgcgtcg gtacgggttc gttggcgacc aaactcgact gcaggacgac
      301 ccgcgccgct gcatcgacag cctgtacctc gtagcgaatg gcaacgatcg cgcgttggac
      361 gaacgagacc agtcgtgtcg agcggatggc caccgactgc cttgcgggcg acgtccactg
      421 cactcgccgg tcgaggagac cgtgtcgcat gtccagcacg cgttcgtggt gggtgagtgt
      481 gccgtagcga acgtcgaacg gctcgtcgtc gacgagcagg cgcatgatct tgccgttggt
      541 cacgtcgatc agcgtctggc cgtcttcggg atagccgtag ccgacttcgg catacggcaa
      601 cggcaccgtt tcgaagaagc cgttcaagta cgtgccgggg acggcgtgcg gctcgccttc
      661 gtcgagattc ccgcgcaagc cgatgtggcc gttcgagagc gcgaacagcg actccaggtg
      721 cgcgagccgg tcgagatgca gctcgcgctc gacgatgcac cacgcatcgg ttccgaactc
      781 gtgctcgtcg gtcacgggtc ctccaacagc tcgcccaagt ctgacacgac gatgtcggcg
      841 ccatgcgagc gaagcgcggc ggtttggtcg acacgatcga cgccgacgat acagccgaag
      901 ccgccgctgc gccccgcttc gacacccgcg agtgcgtcct cgaacaccgc cgcgtgctgc
      961 ggtgcgacgt cgagcaacgc ggccgcggcg aggaacatgt ccggggcagg tttgccgcgg
     1021 agattccgtt gcgcggcaac gacgccgtcc acgcgcgcgt cgaagagatg gtcgatgtgc
     1081 gcagcttcca ggatggcgcg gcagttcttg ctggccgaaa ccaccgcgcg tgggaggcgc
     1141 gcatcacgca ctgcctcgac gaagcggacc gaagacgcgt atacgtcgac acctcccgcg
     1201 tcgatcatct cgagcaggag ttcgttcttg cgggtcgcga ggcccgcaac ggtctcggcg
     1261 gacgggggat cgtccgcgga tccgtccgga agatcgatgt tgcgcgaggc gaggaacgcg
     1321 cgcacgccgt cctcgcgcag cttgccgtcg acgtagcgtg cgtagtcgtc aggcagactg
     1381 aacggccgta ggtcgccgcc gactcgtcgc gcgcgatccg acaggtactg atcgaacatc
     1441 tccttccacg ccgcggcatg aactcttgcc gtctgcgtga ggacaccgtc gagatcgaag
     1501 aggcacgcgc gaatcccgtc gggaagacgc ggggacgcgt tcacgccgtt caccgtctgg
     1561 tcgttccatc cggttcggtg cgagaaacag gaggcgcggg cgcgggcgtt gccaagagct
     1621 ggcatttcct gatccaggcg gcccgcgccg ccgatagaga ccctgaacgc acgacggaat
     1681 tgtgcgaacc cggacatagg gaccacactc atggtgcgct tcggatggcg ccgcaatcga
     1741 ctcggggggg aatcgatgcg tactcaggga cggagtgtcg ctcgcgccgc gatcgcggcg
     1801 agcgggccct ggtggtcgca ggacttcggc gggtgaacgt gcgccggggt ccgcatcagc
     1861 atccgtcgca tgcacgcgac cggcgtcgcg gctacgagct ctgggtgacg tgcgccgact
     1921 gcggcgacgt cgtggtccgc gcggagctgt gcgaactgca cgcgcgcgcc ggggacttca
     1981 cgatcgtgtt cccctgctcg cagtgcgcac ggcgcgacgt ggtacccgtg acgaccgagg
     2041 atgtcctgga gctcgttgcc gcgggcttcg tcatccggcg acagtcggcg gcggccgagt
     2101 tgcgcgagca ccggccgtgc ggcgaaccgt tcgcatggga cgacctgctc gcgttccacg
     2161 aggacctcgc gtccgcgacc gacgtggtcg cgctgctcga ccagacgcgc gttcgatagc
     2221 ccgtcagtcg cgcgacggcc agaggtcgac cggcccgccg acgtagccgc ccgcggcgcg
     2281 tacagcctcg ccccacggcc tgagcagcgc gcagagctcc ccgacgtcgt cgccgagcgc
     2341 ctcgatcgcg ggccgcaggg cgcggtcggt cgcgcactcg atctcttcgc gttcgtggcg
     2401 accggtctcg gtgagggtct cgccgtcgag ccaaccgcgg gcgttcaggc gttgcaccgc
     2461 ggcttcgagc tcctcgtcgt tccacgcgcg ggttcgcacg taggtgcgca acggcatgcc
     2521 gatgtaggcc tccatcagca gaccgatctc gggcgcatcg acgtctgcgc tcgaccacgc
     2581 ggcaacgtgc gcgtcgccgc ggtactcgcg cagcatgtcg ccgaggtgga acaagcgcgt
     2641 ccacggatcg ctcgggtcgt cccactgcgc gcggaggccc gcgaacaacg ggcggccggg
     2701 cggcgcgagc gggtcgacgg cgcgcgtcat gagcgcgatc gcccggtcga cgttcgcgtc
     2761 ggccggcccg aggatgcgca cgagctgtgc gaccgcgccg ttccggcggg ccgcgaagat
     2821 cgtcggcgcg tcggtgaggt tccagcccgc gcgcacgccg tcgacgacga cctccggctt
     2881 gaacacaccg aaggcggcgg cgaccacctc gccttccacc tgccccatca gcgagccgcg
     2941 gctcgtgaag tacgcagatc cgtcgggcat cgccacgttg ttgaacatgc cgggactcgg
     3001 gccgaatccg agcgtgcgat acgcctcgtg gcactcgggc gagaagtaca cctggccgat
     3061 gaccggctcg agcatgccgg ccatggtgcg agaacgattg cgaagttgcg tcgtatccac
     3121 gcgacgagtc tgtacggctc ggggcgcaac gctcaacaat gctttgtcgc cttcgacctg
     3181 ctcgcgtgag gcggctcgga ttgttggccg gcctcaggac tccgcgagtg cctccgcaag
     3241 ctccggcacc gcatctcttt gtcgccttcg acctgctcgc gtgaggcggc ccggattgtt
     3301 ggccggcctc aggactccgc gagtgcctcc gcttcgctcc ggcaccgcaa tgctttgtcg
     3361 ccttcgacct gctcgcgtga ggcggttcgg attgttggcc ggcctcagga ctccgcgagc
     3421 gcctccgcaa gctccggcac cgcaactctt tgtcgggagt gcgaagttac tgcgtggcgg
     3481 accagacgac cgcgtcgcac gcggccgccc acgtgacgag ctcggggtcg tcgtcgacga
     3541 ggatgacctg gcgtacgcgg ctgtgggcca ccaacgcgtc gaacacccgg cggcgggcag
     3601 tcggcacgaa ccccgcgaag acgtggtcga ccacgagggg cgggagcatc gtgctcgccg
     3661 agcgttcgtc gtcggcgtcg agcaacgcct gcagcacgcc gggcaggtcg atcggcgcgc
     3721 gggcgacgag gtctcggcgc gatcgttcga ggcgagcctg ttcgacctcg agcgcccgga
     3781 cttcgatcgc gagccggtcg agctcgtcaa cggccctgtc ggtggcgcgg tccgtgtcgc
     3841 tcgcggtgac ggcgcccgcg gcgaccggct ggagacgacg caacttcgtc acgacatcgg
     3901 cgtcggcttc gctgcccaac aacacgctcg cgcgggcgag cgcgtcttgc cggcggcgtg
     3961 cgagcgccgc gggatcggag gtttcgagct cggcgcgcag gtcgatgcag tgttgctcgg
     4021 ccatcgacac gacggtcgcc gcctcgcgga tcgcgaggtc ggcatcggtg ttgccgaagc
     4081 ccgtgccgac cacggcgaga tcctcgtacg tggcggcgcc gaaacggccg aggaagcgct
     4141 cgtagtgctg cttcaactcg tcgacgtcgc gacgggtgcg gcggcggcgc atctggcggt
     4201 gcacctgctc ggcgtagcgc cactcgttgc ggaggcggag cgcttcggcc aggtcggcgg
     4261 gttgcacgcg cgcggcctgc tcgtggcatt cgacgagtcg tgtgcgcgct tcgccggtga
     4321 cgcgctccca cttcgcgagc tcgcgaccga cggtggcgcg cgggcgacgc gcgctgatct
     4381 cggcgtcgag cgcggccaac tcgtcggcga gcgcgagcgc ttcgacctca cggccg
//
LOCUS       DASUGA010000367         2525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_229166, whole genome shotgun sequence.
ACCESSION   DASUGA010000367 DASUGA010000000
VERSION     DASUGA010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229166"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VFX21_07260"
     CDS             complement(<1..471)
                     /locus_tag="VFX21_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5095791.1"
                     /translation="MAVRYARNGDQHLAYRVFGEGAVDLLHHTSLFVSFDSYDDEPHV
                     ARFERRLGAFARVIEFDQRGMGLSDRGVDDYDIETFVDDTLAVLDAAGSTNTILFCPT
                     GAGLVGIALARRAPERVQSLVLFGSYSKLLRSPEYPIGLPPEIVDMFINDNTDPD"
     gene            complement(482..1042)
                     /locus_tag="VFX21_07265"
     CDS             complement(482..1042)
                     /locus_tag="VFX21_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095792.1"
                     /translation="MPTDPFVPADPADRPRQQQNLPPGTALPPARAWRADRPGDLGPG
                     QPTGPLIGSPGPNVGYAYTLAERAAERVEVSEHEDDHDVLPVLAEIAAKRAALFGRAP
                     VIHDIDIAIALLGYDGTADEDFVEIRSLIVHDAGHDYTRRRAVVDTVPEQLLRLKPDE
                     FAAMIDEWRHLTRAKLFPELGRQPTM"
     gene            complement(1124..>2525)
                     /gene="cimA"
                     /locus_tag="VFX21_07270"
     CDS             complement(1124..>2525)
                     /gene="cimA"
                     /locus_tag="VFX21_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX5095793.1"
                     /translation="TLVAFGSTRRVKGKVDSDDTLRHLVEANVGTVCIVGKSWDYHVT
                     EALGTTLDEGVAMVGDSVEFLRGAGMQVLFDAEHFFDGYKRNPEYALRVLESAVTNGA
                     SHLVLCDTNGGALPHEVERCVREVVAYFGNDIVVAVHLHDDGGTGVANALAGVLGGAR
                     QVQGTINGYGERTGNCNLTTIIPNLTLKMGIETIPRDRLDRLTPVAHHVAELVNMPLN
                     PQAPYVGHSAFAHKAGLHTSAIAKRPDAYEHVSPDVVGNGTRFVVSELAGKSTLVMKA
                     KELGLDLDGPQLAEVVERLKQLEHEGYHFEVADGSLELLMRRVAGWRPSWFEIESFRV
                     ITDDATGVDTLTGETQATVMTEATVKVHIDGERHIATAEGNGPVNALDRALRAALNGR
                     VASLEHLHLTDFKVRVLDTNKGTGAVTRVLIDSSDGDATWTTIGVNENIIEASWQALL
                     DSVHFALLRADLARQP"
BASE COUNT          382 a          868 c          863 g          412 t
ORIGIN      
        1 gtccgggtcc gtgttgtcgt tgatgaacat gtcgacgatc tcgggcggca gaccgatcgg
       61 gtactccggc gaccgcagca gcttggagta cgacccgaag agcaccagtg actgcacacg
      121 ctcgggtgcg cgacgggcca gcgcaatacc gacgagaccg gcgccggtcg gacagaacag
      181 gatcgtgttg gtcgagcccg ccgcgtcgag caccgcgagc gtgtcgtcga cgaaggtctc
      241 gatgtcgtag tcgtcgacgc cccgatcgga gagtcccatg ccccgctggt cgaactcgat
      301 cacccgcgcg aatgcaccga ggcgacgctc gaagcgcgcg acgtgcggtt cgtcgtcgta
      361 ggagtcgaat gacacgaaca gcgacgtgtg gtgcaggagg tcgaccgcgc cttcgccgaa
      421 cacgcggtac gcgaggtgtt ggtcgccgtt gcgggcgtag cgcaccgcca tcagtccacg
      481 cttacatcgt gggctggcga cccagctccg ggaagagctt cgcgcgcgtg aggtggcgcc
      541 attcgtcgat catcgccgcg aactcatccg gcttcaggcg caacaactgc tccggcaccg
      601 tgtcgaccac cgcccggcgg cgcgtgtagt cgtgccccgc gtcgtgcacg atgagagacc
      661 ggatctcgac gaagtcctcg tccgcggtgc cgtcgtaccc gagcagcgcg atcgcgatgt
      721 cgatgtcgtg gatgaccggc gcccgcccga acagcgcggc gcgcttggcc gcgatctcgg
      781 cgagcaccgg caacacgtcg tggtcgtcct cgtgctcact gacctcgacc cgttccgccg
      841 cgcgttcggc gagtgtgtac gcgtagccga cgttcggacc cggcgacccg atcagtggtc
      901 ccgtcggctg cccgggcccg aggtcaccgg gccggtcggc ccgccacgcg cgcgccggcg
      961 gcagtgcggt gcccggcggc aggttctgct gctggcgggg acgatcggcg gggtccgcgg
     1021 ggacgaacgg atcggtcggc atcgccccgg aggctacccg ccggcccggt ccgcacgaac
     1081 aaccatggct ccctcgctgc gcgccgaggg gcgccttcgg cgctcacggt tgccgcgcta
     1141 ggtccgcacg gagcaacgcg aagtgcacag aatcgaggag tgcctgccag gacgcctcga
     1201 tgatgttctc gttgacgccg atcgtcgtcc acgtcgcgtc gccgtcgctc gagtcgatca
     1261 gcacccgcgt gaccgcaccc gttcccttgt tcgtgtcgag gacgcgcacc ttgaagtcgg
     1321 tgaggtgcaa gtgctcgagc gacgcgaccc ggccgttgag cgcagcgcgc agcgcgcggt
     1381 cgagcgcgtt caccggaccg ttcccttcgg cggtcgcgat gtgccgctcg ccatcgatgt
     1441 gcaccttgac cgttgcctcg gtcatgaccg tcgcctgcgt ctcgccggtc agcgtgtcga
     1501 cacccgtggc gtcgtcggtg atcacccgga acgactcgat ctcgaaccac gacggccgcc
     1561 aaccggcgac gcggcgcatg agcagttcga gcgaaccgtc ggcgacctcg aagtgatacc
     1621 cctcgtgctc cagctgcttg agccgctcga cgacttccgc gagctgcggg ccgtcgaggt
     1681 cgaggccgag ctccttcgcc ttcatcacga gggtcgactt gccggcgagc tccgacacca
     1741 cgaagcgggt gccgttgccg acgacgtcgg gcgacacatg ctcgtaggcg tcgggccgct
     1801 tggcgatggc gctcgtgtgc aacccggcct tgtgcgcgaa cgccgagtgc ccgacgtacg
     1861 gcgcctgcgg gttgagcggc atgttcacca gctccgcgac gtggtgggcg accggtgtga
     1921 gccggtcgag tcggtcgcgc gggatcgtct cgatacccat cttcagcgtg aggttcggga
     1981 tgatcgtcgt gaggttgcag ttgccggtgc gctcgccgta gccgttgatc gtgccctgta
     2041 cctggcgggc gccgccgagc acacccgcga gcgcgttggc aacgccggtg ccaccgtcgt
     2101 cgtggaggtg cacggcgacg acgatgtcgt tcccgaagta ggcgacgacc tcacgcacgc
     2161 aacgctcgac ctcgtgcggc agcgcaccac cgttggtgtc gcacagcacg agatgcgacg
     2221 caccgttcgt gaccgccgac tcgagcactc gcagcgcgta ctcggggttg cgcttgtagc
     2281 cgtcgaagaa gtgctccgcg tcgaagagca cctgcatgcc cgcgccgcgc aggaactcga
     2341 ccgaatcgcc gaccatcgcg acgccttcgt cgagcgtcgt cccgagcgct tcggtgacgt
     2401 ggtagtccca cgacttgccg acgatgcaaa ccgtgccgac gttcgcctcg accagatggc
     2461 gcagcgtgtc atcgctgtcg accttccctt tgacccgacg cgtcgaaccg aacgcgacga
     2521 gcgtg
//
LOCUS       DASUGA010000368         5415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1685758, whole genome shotgun sequence.
ACCESSION   DASUGA010000368 DASUGA010000000
VERSION     DASUGA010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685758"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="VFX21_07275"
     CDS             complement(<1..1087)
                     /locus_tag="VFX21_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011598699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5095794.1"
                     /translation="MKTEAPANNTRLAILLAMAMFVLVVDTSLMNVSISSVVRDLDTT
                     VSGVQSAIALEALVSAAFILIGSKVGDLIGRKRAYVLGLLGYALGAASMAIGQSLTAI
                     IIFWAIIGGLGASLLLPSMQSLIHGNFEGAAQKKAYALVGAAAAIAAAVGPLIGGFIT
                     TYLSWRIAFLLEDVIIIIVLSGIKLVKDAPYTGSREVDVVGAVFSVVGMGGIVLGILV
                     WQEGGEAVGALIAIGAIAMGALVWWLQKRKRAGKAALIDPGLFSSKVFRLGISQQMMQ
                     QIALGGTMIALPIFLQMVLEYNAMEAGLTIAPLSLSMFAIALLAGKRAGKRRASAVVR
                     MGFGLLTLGLVILLPIVPRAESGWPLVI"
     gene            complement(1104..1523)
                     /locus_tag="VFX21_07280"
     CDS             complement(1104..1523)
                     /locus_tag="VFX21_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015747029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095795.1"
                     /translation="MGSEFPDETRPSAWAVGWIFFAATMMIMIGVFHAIAGLIAIFDD
                     EFYVTTRDYVFQFDTTGWGWIHLILGIIVAVSGGYLLTGSVFARTVGVILAMVSMAAG
                     FAWIPYYPIWGVIIVAMAVSVIWALTAHGRDIQAEMD"
     gene            1607..2074
                     /locus_tag="VFX21_07285"
     CDS             1607..2074
                     /locus_tag="VFX21_07285"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX5095796.1"
                     /translation="MPIELPDFQLPNFDEKYDEKIAKSLEGSTDFAGGLMRFLGIRHV
                     EVGAGVLVAEIDMRDELKTPAGNLHGGALAALCDHVLGTVCYPVMVRGAWAATTEFKL
                     NYLAPVTEGTLRATAQIISMTRRTAVVRIDVVNGDRLVCTAQGTVLLMEPKPK"
     gene            complement(2077..4410)
                     /locus_tag="VFX21_07290"
     CDS             complement(2077..4410)
                     /locus_tag="VFX21_07290"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX5095797.1"
                     /translation="MAKPFKGIINVDVRDSTPDWEPYAPVTAPDGAPNVLIVLYDDTG
                     LASWSPYGGRIDMPTMQRFADNGLTYTQWHTTALCSPTRSCILTGRNHHQNGYACIAE
                     GATGFPGSNAHIPWENAFLAQVLRDSGYGTFWVGKNHNVPSEDWDMGGSKQTWPLARG
                     FDRFYGFLGGETDQWYPTLVNDNHYVDQPYLPEDGYHLSKDLADQAIRMIRDTRQTAP
                     DKPWYMFYCPGANHAPHHAPKEYIDKYKGMFDDGYEAYREWVLPRMIEKGILPPGTEL
                     TDLNPMTEGTFSPADMVKPWAECSDDEKRLYARMAEVFAAYSEYTDVQVGRIVDYLEE
                     SGQLDNTIVFYCADNGASGEGTPFGSVNENKFFNGFPDDLEQNLTMLDKLGGPETYNH
                     YPTGWAVGFSTPFRMFKRYSYQGGICDPLVISWPKGIKARGEVRDQYHHAIDIVPTIL
                     DCIGMEFPERVLGYAQTPLPGVSMKYSFDDAGAPTQKVTQYYAMLGTRGLWHQGWKVV
                     SEHGPLSSIGNFDKDNWQLFHTDEDRAEAHDLSSQHPEKVTELVNLWFAEAGKNDVLP
                     LDDRRALEILLDERPVPLPPRGLYRYYPNTTEVSERTAASVRGRSYKILATVDITDAG
                     ADGVLYSHGARFGGHVLFVKDRKLHYVYNFLGIPPEQHLVSSVEITPGRRVLGVDFAK
                     ESVDDHGSSHGTATLYIDGDAAGTDEIRTQPGHFALCGEGMCIGRDSGDPISAAYASE
                     RFQFEFAGGEIKVVEVSVGDDQYVDLEKEALAMMARE"
     gene            4585..5001
                     /locus_tag="VFX21_07295"
     CDS             4585..5001
                     /locus_tag="VFX21_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095798.1"
                     /translation="MTAGWSLARAELRARPWSALVIAVVGGLALGLALAGVAGARRGP
                     AAWDDLVARFRASDFSVFPQDPERLDELEQTLAGSDAVAATSRSAFVLLGAVEADGTI
                     SDRGVAAGMVDIAGDRSVERPAVVRAGCRRPRSRSR"
     gene            4971..>5415
                     /locus_tag="VFX21_07300"
     CDS             4971..>5415
                     /locus_tag="VFX21_07300"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5095799.1"
                     /translation="MPEAAFEVAVNDAVARRRHVEPGDTIRLAVFGAGQVDGVGRTSD
                     PPAHGQFDFTVTGIVRQPGDLEFQAEAQPGTVYERDDEMHWYSAALWSRFDGDIANYG
                     TGVFARLRPGADTEALADELRTVDRNAVVDDEPDSADIFTTIARTI"
BASE COUNT          892 a         1792 c         1828 g          903 t
ORIGIN      
        1 ggatgacgag cggccacccg gactccgcgc gcgggacgat cggcaggagg atgacgagcc
       61 cgagcgtcag gaggccgaag cccatgcgca cgaccgcgct ggcgcggcgc ttcccggctc
      121 gcttcccggc gagcagcgcg atggcgaaca tgctcagcga gagcggcgcg atcgtgaggc
      181 cggcttccat cgcgttgtac tcgagcacca tctgcaagaa gatcgggagc gcgatcatcg
      241 tgccgcccag cgcgatctgc tgcatcatct gctgcgagat cccgagccgg aacaccttcg
      301 acgagaacaa ccccgggtcg atcagcgcgg ccttgcccgc gcgcttgcgc ttctgcagcc
      361 accacaccag cgcgcccatc gcgatcgcgc cgatggcgat gagcgcaccg actgcttcgc
      421 caccctcctg ccacacgagg atgccgagca cgatgccgcc catgccgacg accgagaaga
      481 ccgcgccgac gacgtcgact tcacgagacc cggtgtaggg cgcgtccttc acgagcttga
      541 tgcccgacag gacgatgatg atgatcacat cctcgagcaa gaacgcgatg cgccacgaca
      601 gataggtcgt gatgaagccg ccgatgagcg ggccgacggc cgctgcgatc gcggcggccg
      661 cgccgacgag cgcgtacgcc ttcttctgcg cggcaccttc gaagttgccg tggatgagcg
      721 actgcatcga cggcagcagg agcgacgcgc cgagcccgcc gatgatcgcc cagaagatga
      781 tgatcgcggt caggctctgg ccgatcgcca tcgacgccgc gcccagggcg tagccgagca
      841 agccgaggac ataggcgcgc ttgcgcccga tgaggtcgcc gaccttgctg ccgatgagga
      901 tgaacgcggc cgagacgagc gcctcgagcg cgatcgcgga ctggacaccg ctgaccgtcg
      961 tgtcgaggtc gcgcacgacc gacgagatcg acacgttcat cagtgacgtg tcgacgacga
     1021 gcacgaacat cgccatcgcg agcaggatcg cgaggcgagt gttgttcgcc ggcgcctcgg
     1081 ttttcatgca gtgactcgac tcgtcaatcc atctcggcct ggatgtcgcg gccgtgcgcg
     1141 gtcagtgccc agatcacgct gacggccatg gcgacgatga tgacacccca gatcgggtag
     1201 tacggaatcc acgcgaagcc ggcagccatg ctcaccatcg ccaagatgac gccgaccgtg
     1261 cgcgcgaaga ccgaaccggt gagcaagtag ccgccgctca cggcgacgat gatgccgagg
     1321 atgaggtgga tccaccccca gcccgtcgtg tcgaactgga acacgtagtc gcgcgtcgtg
     1381 acgtagaact cgtcgtcgaa gatggcgatc aaccccgcga tggcgtggaa cacgccgatc
     1441 atgatcatca tcgtcgccgc gaagaagatc caaccgaccg cccacgcgct cggacgagtc
     1501 tcgtcgggga attcggaacc catgtggtgc tcctcgggtc aggtaacagc cagaatcttc
     1561 gcactcgccg tccgccgaac cgtggacctc gcgtacgctg ccgtacgtgc cgatcgagtt
     1621 gcccgatttc cagctgccga atttcgacga gaagtacgac gagaagatcg ccaagagtct
     1681 cgaagggtcg accgacttcg cgggcgggct gatgagattc ctcggcatcc gccacgtcga
     1741 agtcggcgcg ggcgtcttgg tcgccgagat cgacatgcgc gacgagctga agaccccggc
     1801 cgggaacctc cacggcggcg cgctggccgc gttgtgcgac cacgtgctcg ggacggtctg
     1861 ctacccggtc atggtgcgcg gcgcgtgggc ggcgacgacc gagttcaagc tcaactacct
     1921 cgcgccggtc accgagggca cgttgcgcgc gaccgcacag atcatctcga tgacgcgccg
     1981 caccgcagtc gtacgcatcg acgtcgtgaa cggagaccgt ctcgtgtgca ccgcgcaggg
     2041 cacggtgctc ttgatggagc ccaagccgaa gtagcgctac tcgcgcgcca tcatcgccag
     2101 cgcttccttc tcgaggtcga cgtactggtc gtcgcccacg ctcacctcga cgaccttgat
     2161 ctcgccgccc gcgaactcga actggaatcg ctccgatgcg tacgcggcgc tgatcgggtc
     2221 gccgctgtcg cgcccgatgc acatgccttc gccgcacagc gcgaagtgac cgggctgggt
     2281 acggatctcg tcggtaccgg ccgcgtcgcc gtcgatgtac aacgtcgcgg tgccgtgcga
     2341 cgagccgtgg tcgtcgacgc tctccttggc gaagtcgaca ccgagcacgc gccgccccgg
     2401 cgtgatctcg acgctcgaca cgagatgctg ctcgggcggg atgccgagga agttgtagac
     2461 gtagtgcagc ttgcgatcct tgacgaacaa cacgtgcccg ccgaagcgcg cgccgtgtga
     2521 atacagcacg ccgtccgcgc ccgcgtcggt gatgtcgacc gtggccagga tcttgtacga
     2581 ccggccgcgc accgatgccg ccgtgcgctc cgacacctcc gtcgtgttcg ggtagtaccg
     2641 atagagaccg cgagggggca ggggaacggg tcgctcgtcc aagaggatct cgagcgcacg
     2701 gcggtcgtcg agcggcagca cgtcgttctt cccggcttcc gcgaaccaga ggttcacgag
     2761 ctcggtgacc ttctccgggt gctgcgacga caggtcgtgc gcttcggccc ggtcctcgtc
     2821 ggtgtggaac agctgccagt tgtccttgtc gaagttcccg atgctcgaca acggaccgtg
     2881 ctcggagacg actttccagc cttggtgcca caggccgcgc gtcccgagca tggcgtagta
     2941 ctgcgtcacc ttctgcgtcg gcgcgccggc gtcgtcgaag ctgtacttca tcgacacgcc
     3001 cggcagcggt gtctgcgcgt agccgagcac gcgctcgggg aactccatgc cgatgcagtc
     3061 gaggatggtc ggcacgatgt cgatcgcgtg gtggtactgg tcgcggactt cgccgcgcgc
     3121 cttgatgccc ttcggccacg agatcacgag cgggtcgcag atgccacctt ggtaggagta
     3181 gcgcttgaac atccggaacg gcgtcgagaa cccgaccgcc caaccggttg ggtagtggtt
     3241 gtacgtctcg ggaccgccga gcttgtcgag catcgtcagg ttctgctcga ggtcgtcggg
     3301 gaacccgttg aagaacttgt tctcgttgac cgaaccgaag ggtgtgccct cacccgacgc
     3361 gccgttgtcc gcgcagtaga acacgatcgt gttgtcgagc tggcccgact cctcgaggta
     3421 gtcgacgatg cgacccactt ggacgtcggt gtactccgag tacgccgcga acacctcggc
     3481 catgcgcgcg tagaggcgct tctcgtcgtc ggagcactcg gcccacggct tcaccatgtc
     3541 ggcaggtgag aacgtgccct cggtcatcgg gttgaggtcg gtgagctcgg tgcccggcgg
     3601 gaggatgccc ttctcgatca tgcgcggcag cacccactcg cggtacgcct cgtagccgtc
     3661 gtcgaacatc cccttgtact tgtcgatgta ctccttcggc gcgtgatgcg gcgcatggtt
     3721 cgcgcccggg caatagaaca tgtaccaggg cttgtcgggc gcggtctgcc tcgtgtcgcg
     3781 gatcatgcgg atcgcttggt ccgcgaggtc cttcgacagg tgatagccgt cttcgggcag
     3841 gtacggctgg tcgacgtagt ggttgtcgtt gacgagcgtc ggataccact ggtcggtctc
     3901 gccgccgagg aacccgtaga accggtcgaa gccgcgcgcg aggggccacg tctgcttcga
     3961 tccgcccatg tcccagtcct cggacgggac gttgtggttc ttgccgaccc agaacgtgcc
     4021 gtatccgctg tcgcgcagca cttgcgcgag gaacgcgttc tcccacggga tgtgcgcgtt
     4081 cgagcccggg aatccggtcg caccttcggc gatgcacgcg tacccgttct ggtggtgatt
     4141 gcgaccggtg aggatgcacg aacgcgtcgg cgagcagagc gcggtcgtgt gccactgcgt
     4201 gtaggtcagg ccgttgtcgg cgaagcgctg catcgtcggc atgtcgatgc ggccgccgta
     4261 cggcgaccac gacgcgagac cggtgtcgtc gtagagcacg atgagcacgt tcggtgcacc
     4321 gtcgggtgcg gtgaccgggg cgtacggctc ccaatcgggc gtcgagtcac gcacgtcgac
     4381 gttgatgatc cccttgaacg gcttggccac gacggactcc ctgaaacgcg cgattgggcg
     4441 cccatgctct ctcccgggcg agttcgcgtt cgtcaccccg tgcgggtgac gcgagctgcg
     4501 tgaaggaaag gggtgcctgc acccctcgcc gcactcatgc ctccggtgtg tcgatcacac
     4561 gccgcgctcg gtaccgtgcc gagggtgaca gcgggctggt cattggcgcg agcagagctg
     4621 cgcgcccgtc cgtggtcggc gctcgtcatc gcggtggtgg gcgggctggc gctcgggctc
     4681 gcactggccg gcgtggccgg tgcccgtcgc ggaccggccg cgtgggacga cctcgtcgca
     4741 cgcttccgcg cgtcggactt ctcagtcttc ccacaggatc ccgaacgact cgacgagctc
     4801 gagcagacgc tcgcgggcag tgacgcagtc gcggccacga gccgaagcgc gttcgtgctc
     4861 ctcggcgcgg tcgaggcgga cggcacgatc tcggaccgtg gtgtcgcggc gggcatggtc
     4921 gacatcgccg gcgaccgatc ggtcgagcga ccagctgtcg ttcgcgccgg ttgccggagg
     4981 ccgcgttcga ggtcgcggtg aacgacgcgg tcgcgcgacg acgtcacgtc gaaccgggcg
     5041 acaccattcg gttggcggtg ttcggtgccg gccaggtcga cggcgtgggg cggacgtcgg
     5101 atccgccggc gcacggccag ttcgacttca cggtcaccgg catcgtccgc caacccggcg
     5161 atctcgagtt ccaggccgag gcccagccag gcaccgtgta cgagcgcgac gacgagatgc
     5221 actggtactc ggcggcgctg tggtcgcgct tcgacggcga catcgcgaac tacggcaccg
     5281 gcgtcttcgc gcgtctccgc cccggtgcgg acactgaggc actcgcggac gagttgcgca
     5341 cggtcgatcg caacgcggtc gtcgacgacg aacccgactc ggccgacatc ttcaccacga
     5401 tcgcgcgcac gattg
//
LOCUS       DASUGA010000369         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_747900, whole genome shotgun sequence.
ACCESSION   DASUGA010000369 DASUGA010000000
VERSION     DASUGA010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747900"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="VFX21_07305"
     CDS             <1..1237
                     /locus_tag="VFX21_07305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5095800.1"
                     /translation="LRRADPTLRVVVCERETVGFGASGRNGGWCSAFFAGARAATEKR
                     HGRDAVIAMQRACIATLDEIEDVIGKEGIACDWARGGSLTVATLPAHRHRLEHSLADH
                     RSYGFGDADWTWLDPPAAQARIGCTPNLGALATPHCAAIHPKKLVLALGDVVDGTGVT
                     IHEHTAVSAIEPHRVVTDHGVVRAEVVVRATEAFTPDFEGDLHRALVPVYSLMIATEP
                     LPASFWAEAGLADRATFADARHMVIYGQRTADDRFAFGGRGAPYHFGSKVRSAYDTNA
                     RVFDAIEQTLRSLFPAMDGAVTHQWGGAVAIPRDWYPSVGFDRATGHACAGGYVGDGV
                     AITNLAGRTLADLVLGHDTELTTLPWVGHRSPRWEPEPLRWAGINAGANLAASLDRAE
                     ASGRSARVRRRLAGALLGH"
     gene            1237..1683
                     /locus_tag="VFX21_07310"
     CDS             1237..1683
                     /locus_tag="VFX21_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095801.1"
                     /translation="MTEHARVVGRRVVVIAVAVLQLFFVVRAYWSAHQEFGFHMFNES
                     TTWQADIVRVTADGQRIPVTQQWDGYAWPELVHTRYLTYPEVRQHADAGIENQLAFLD
                     EALDYVASHTPRDGETLYLEATVTYWRNTHAPQTRVLRSDMRDVAQ"
     gene            1680..>2135
                     /locus_tag="VFX21_07315"
     CDS             1680..>2135
                     /locus_tag="VFX21_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095802.1"
                     /translation="MNAFDELLGRRVSMRAMALLRIAVGPIVLLHLQPFLEDARDGVI
                     YRDAFYAKYVSWYPELPRSLYIALLWAAAVAAVAMTIGLWTRFNTVVAFGIVAYNVFL
                     STTHFHNNRAYLLIVLGMLALAPCGRELSFDAWWRSRNGLPVLPTDAPGW"
BASE COUNT          296 a          728 c          754 g          357 t
ORIGIN      
        1 cttgcggcgg gccgatccga cgttgcgtgt cgtcgtgtgc gagcgcgaga ccgtcgggtt
       61 cggtgcgtcc ggccgcaacg gcggatggtg ctcggcgttc ttcgccggtg cgcgcgccgc
      121 gacggagaag cggcacggtc gcgacgccgt gatcgcgatg caacgcgcgt gcatcgcgac
      181 gctcgacgag atcgaggacg tcatcggcaa ggagggcatc gcgtgcgact gggcgcgcgg
      241 tggatcgctg accgtcgcga cgcttcctgc gcaccggcat cggctcgagc actctctcgc
      301 cgaccaccgt tcctacggct tcggcgacgc ggactggacg tggctcgatc cgccggcggc
      361 gcaggcgcgc atcggatgca cacccaatct cggcgcgttg gcgacgcccc actgtgcggc
      421 gatccacccg aagaagctgg tgctcgcgct cggagatgtc gtcgacggca cgggcgtcac
      481 catccacgag cacaccgccg tctccgcgat cgagccgcat cgggtcgtca ctgatcacgg
      541 cgtggtccgc gccgaggtcg tggtgcgcgc aacggaagcg ttcacgcccg acttcgaagg
      601 cgacctgcac cgcgcactcg tgcccgtgta ctcgttgatg atcgcgacgg agccgttgcc
      661 ggcgtcgttc tgggccgaag cagggttggc cgaccgcgcg acgttcgcgg acgcacgtca
      721 catggtgatc tacgggcagc gcaccgccga cgaccggttc gcgttcgggg gcagaggtgc
      781 gccgtatcac ttcggttcga aggtgcgcag cgcgtacgac acgaacgcgc gcgtgttcga
      841 cgcgatcgag cagacgctgc ggtcgctctt ccccgcgatg gacggcgcgg tgacgcatca
      901 gtggggtggc gcggtcgcga tcccgcgcga ctggtatccg tcggtcggct tcgatcgcgc
      961 caccggtcac gcgtgcgcgg gcggatacgt cggcgacggc gtcgcgatca cgaacctcgc
     1021 gggccgcacg ctcgcggacc tcgtcctcgg gcacgacacc gagctgacga cgttgccgtg
     1081 ggtcggccac cggtcgcccc ggtgggagcc cgaaccgctg cggtgggcgg ggatcaacgc
     1141 gggtgcgaat ctcgcggcat cgctcgaccg cgcggaggcg tcgggccgct cggcgcgcgt
     1201 ccggcggcgc ctggccggcg cgctgctcgg ccactgatga cggagcacgc acgcgtcgtc
     1261 ggccgaaggg tcgtcgtcat cgcggtcgcc gtgctccaac tgttcttcgt ggtgcgcgcg
     1321 tactggtccg cgcaccagga gttcggattt cacatgttca acgagtcgac cacgtggcag
     1381 gccgacatcg tgcgcgtcac cgccgacggc caacgcatcc cggtcacgca gcagtgggac
     1441 gggtacgcgt ggccggagct cgtgcacacc cgttacttga cctatcccga ggttcgtcag
     1501 cacgccgacg cgggcatcga gaaccagctc gcgttcctcg acgaggccct cgactacgtc
     1561 gcgtcgcaca cgccgcgcga cggcgagacg ctctacctcg aggcgaccgt cacgtactgg
     1621 cgcaacacgc atgcgccgca gacccgcgtg ttgcgcagtg acatgcgaga cgtcgcgcag
     1681 tgaacgcgtt cgacgaattg ctcggccgcc gcgtgagcat gcgcgcgatg gcgctgctgc
     1741 gcatcgcggt cgggccgatc gtgctgttgc acctgcagcc cttcctcgag gacgcacgcg
     1801 acggggtgat ctaccgcgac gcgttctacg cgaagtacgt gtcgtggtac cccgagttgc
     1861 cacgctcgct gtacatcgcg ttgttgtggg cggcggctgt tgctgcggtc gcgatgacga
     1921 ttgggctgtg gactcggttc aacacggtgg ttgcgttcgg catcgttgct tacaacgtct
     1981 ttctgtcgac gacccacttc cacaacaacc gcgcgtactt gctcatcgtg ctcgggatgc
     2041 tcgcgctcgc gccgtgcggc cgggagctct cgttcgatgc gtggtggcgc tcgcgcaacg
     2101 gcctccccgt gctcccgacc gatgcgccgg gctgg
//
LOCUS       DASUGA010000370         3162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_509662, whole genome shotgun sequence.
ACCESSION   DASUGA010000370 DASUGA010000000
VERSION     DASUGA010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509662"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="VFX21_07320"
     CDS             <1..818
                     /locus_tag="VFX21_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011604965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5095803.1"
                     /translation="APREIHLEPRARGVDLAVQGITVRFGGVVAVDDVSFSVSPGEVV
                     GLIGPNGAGKTTIIDAVTGFVPIAGGTITLDARRLDGLNATKRARLGLRRSFQSLELF
                     EDVSVEENIRAGSDRLASRWSWFTDLFWPGEHAMPPTAVAAVQAFELEPLLDQTPEEL
                     PYGRRRLVGIARTVASGPSVVMLDEPAAGLDERESAELARLIRKLADERNMAVLLVEH
                     DVGLVMSTCDRIVVIDFGRVIATGAPSEIRSDQAVRDAYLGHAEGEVVEEVTT"
     gene            878..1522
                     /locus_tag="VFX21_07325"
     CDS             878..1522
                     /locus_tag="VFX21_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011601582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5095804.1"
                     /translation="MPVVRDINLHVGAGEVVALIGANGVGKTTTLLTLAGELTPMAGE
                     VSFLGRGTTAPMHARCRNGLGYVTEERSVIMDMSVADNLRLARVKRNVACEYFPQLER
                     IMSRRAGLCSGGEQQMLSLARALGRNPKVLLADELSLGLAPLVVTNLLQAVRDAATNL
                     GIGVLLVEQHVRQALKIADRVYLMERGHIALHGTAAEVVGQLDKIEAAYLTGSA"
     gene            1661..>3162
                     /locus_tag="VFX21_07330"
     CDS             1661..>3162
                     /locus_tag="VFX21_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX5095805.1"
                     /translation="MTVTAVAMLAVLPASAQSKQPAPPPQDVARYILPPGNYGGLPTT
                     NESLDQLPLYDALTPLRGNVSATDIQQNFLPENFEPVGATQEIDTGRPGVRVLYDEYG
                     VAHVYGRTREDVAFGAGWVTARDRMLLLQLGRFPARVAVADVPGIDAFSLVTSAQSFV
                     PSAATEALVTQQIDLLIQTYGDKGRQIIADAQAEADGVNAYAAANNIDLTPPVTVNDV
                     IAVTAFIGSIFGAGGGGEARNADLLAKLRQGLGVQRGDAAWDDAMLFDDPEAPTTIRQ
                     HFNYGPLTGGPVRGSVVVDANSIVPLDPRAPASAAASTVDPTAAPPGRQASNFLVVDP
                     KRSATGNTLAVMGPQLGYYYPEIVQQIHLVGPGYEAQGAAVPGMAMYILIGRTHDYAW
                     SLTSANHDVRDVFAEQLCEPDGSAPTRASDHYLFDGECRPFVEFDAGLLNGTPLRYPT
                     SVHGPVIGTATVQGAPYALTRKRSTFGRDGLNLAALKDMTDGVATTAQKF"
BASE COUNT          504 a         1097 c         1078 g          483 t
ORIGIN      
        1 aggcgccgcg tgagatccac ctggagccgc gagcgcgcgg cgtcgacctg gcagtgcagg
       61 ggatcacggt ccgcttcggg ggcgtggtcg cggtcgacga cgtgtcgttc tcggtctcac
      121 cgggtgaggt ggtcgggctc atcgggccga acggcgcggg caagacgacg atcatcgacg
      181 cggtcacggg cttcgtcccg atcgcgggcg gcacgatcac gctcgacgcg cgtcgcctcg
      241 acggcctcaa cgcgaccaag cgcgcgcgtc tcgggctgcg gcgatcgttc cagtcactcg
      301 aactgttcga ggacgtgagc gtcgaggaga acatccgcgc cggcagcgac cgcctcgcgt
      361 cgcgctggtc gtggttcacc gatctgttct ggcccggtga acatgcgatg ccgccgaccg
      421 cggtcgccgc ggtgcaggcc ttcgagctcg aaccgctcct cgaccagacc cccgaggagc
      481 tgccgtacgg ccgccgccgg ctcgtcggca tcgcgcgcac ggttgcgtcg ggcccgtcgg
      541 tcgtcatgct cgacgagccc gccgccggcc tcgacgaacg cgagagtgcc gagctggcgc
      601 ggctcatccg caagctcgcc gacgaacgca acatggccgt gctcctcgtc gagcacgacg
      661 tcggcctcgt gatgtcgacg tgcgaccgca tcgtcgttat cgacttcggc cgggtgatcg
      721 cgaccggcgc gccgtcggag atccgcagcg accaggcggt gcgcgacgcc tacctcggcc
      781 acgccgaagg cgaggtcgtc gaggaggtga cgacgtgagc gcgacgttgc tcgagacgtt
      841 gatcgaggcg cgcggagtgt cggccggcta cggaaagatg cccgtcgtcc gagacatcaa
      901 cctgcacgtc ggcgccggcg aagtcgtcgc cctgatcggc gcgaacggcg tcggcaagac
      961 gaccacgctg ctcaccttgg ccggcgagct gacgccgatg gccggcgagg tcagctttct
     1021 cggacgcggc accaccgcac cgatgcatgc ccgctgccgc aacggactcg gctacgtcac
     1081 cgaagaacgc tccgtgatca tggacatgtc cgtcgccgac aacttgcggc tggcccgcgt
     1141 gaaacgcaac gtcgcctgcg agtacttccc gcagctcgag cgcatcatga gccggcgcgc
     1201 gggactgtgc tcgggtggcg agcagcaaat gctgtcgctg gcccgggcgc tcggccgcaa
     1261 tccgaaggtg ctgctcgccg acgagctgtc actggggctc gcgcccttgg tcgtcacgaa
     1321 tcttctccag gccgtgcgcg acgcggcgac gaatctcggg atcggcgtcc tgctcgtcga
     1381 gcaacacgtg cgccaggcgc tgaagatcgc cgatcgcgtc tacctcatgg agcgcggcca
     1441 catcgcgctg cacggcacgg cggccgaggt cgtgggccag ctcgacaaga tcgaagccgc
     1501 atacctgacg ggcagcgcct gagactcgcg tcgcgtcgga cacggatgcg cggcgcatta
     1561 gcgcgaactt cgtcgccgta cctaaggtgc ggctcgcggg tatgagacac aaacgaggtc
     1621 tggccccgtg cgacgtatca ggtggacacg aatcgccgtc gtgacagtga cggcagtcgc
     1681 gatgctcgcg gtgctgcccg cgagcgcaca gagcaagcag cctgcgccgc cgccgcagga
     1741 tgttgcgcga tacatcctcc cgcccgggaa ctacggcggc ctgccgacga cgaacgagtc
     1801 gctcgatcag cttccgttgt acgacgcgtt gacgccgctg cgcggcaacg tcagcgcgac
     1861 cgacatccag cagaacttcc tgccggagaa cttcgagccc gtcggtgcaa cacaggagat
     1921 cgacaccggt cgcccggggg tccgcgtcct gtacgacgaa tacggcgtcg cgcacgtcta
     1981 cggacgcaca cgcgaagacg ttgccttcgg tgcggggtgg gtcaccgcgc gcgaccggat
     2041 gctgctgttg cagctcgggc gctttccggc tcgtgtcgcg gttgccgacg tgccgggtat
     2101 cgacgccttc tcgctcgtga cgagcgcgca gtcgttcgtg cccagcgccg caaccgaggc
     2161 actggtcacg cagcagatcg acctcttgat ccagacctac ggcgacaagg gccgccagat
     2221 catcgccgac gcacaggccg aagcggacgg cgtcaacgcg tacgcggcgg cgaacaacat
     2281 cgacctcacc cctccggtga cggtgaacga cgtgatcgcc gtcactgcgt tcatcggctc
     2341 gatcttcggc gccggtggcg gcggcgaagc gcgcaacgcc gacctcctgg cgaagttgcg
     2401 gcagggcctc ggtgtccagc gcggtgacgc cgcgtgggac gacgcgatgc tcttcgacga
     2461 tcccgaggcg ccgacgacga tccggcagca cttcaactac ggaccgctga ccggtggccc
     2521 cgtccgtggt tcggtcgtcg tcgatgcaaa ctccatcgtt cccctcgacc cgcgggcgcc
     2581 ggcgagcgcg gccgcgtcga cggtcgaccc cacggccgcg ccgcccggcc gtcaggcgtc
     2641 gaacttcctg gtcgtcgacc cgaagcgctc ggcgacggga aacacgctcg cggtgatggg
     2701 tccgcagctc ggctactact acccggagat cgtccagcag atccatctcg tcggcccggg
     2761 ctacgaggcg caaggtgcgg ccgttcccgg catggcgatg tacatcctga tcggccgaac
     2821 gcacgactac gcgtggagcc tgacgtcggc gaatcacgac gtgcgcgacg tcttcgccga
     2881 acaattgtgc gaacccgacg gttcggcgcc gacccgtgcg tcggatcatt acctgttcga
     2941 cggcgaatgc cggcccttcg tcgagttcga cgccggtctc ctgaacggca cgcccttgcg
     3001 ctacccgacg tcggtgcacg ggccggtgat cgggaccgcg acggtgcagg gtgcgccgta
     3061 cgcactgacc cgcaagcgct cgaccttcgg tcgcgacggg ctcaacctcg cggcgttgaa
     3121 ggacatgacc gatggtgtcg cgaccaccgc gcagaagttc tt
//
LOCUS       DASUGA010000371         2613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1594823, whole genome shotgun sequence.
ACCESSION   DASUGA010000371 DASUGA010000000
VERSION     DASUGA010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594823"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="VFX21_07335"
     CDS             <1..1128
                     /locus_tag="VFX21_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5095806.1"
                     /translation="YKRLRDEAPLFYNEEYDFYAVSRYDDVERGLVDAKHHISGRGGI
                     LELIKADIEIPPGTLIFEDPPVHTIHRSLLSRVFTPRRVAALEPQIREYCARCLDPLE
                     GKDELDFILDFGAEMPMRVIGMLFGIPEEDQFKVKASSDARLRTEAGQPMDVGENPTF
                     GSENFEAYIDWRIDHPSDDLMTDLLTAQFQDENGETRTLTRDEAVMYTTVVAGAGNET
                     TTKLIGWAGKTLADYPDQRRQLVEDRSLIPNAIEELLRFEPPGPHIGRYVPEPVEWYD
                     TTIPEGSAILFVNASANRDERKFGDTADRFDIHREIKTHLAFGYGIHFCLGAALARLQ
                     GRIALDELLTRWPEWEVDIARAKIAPTSTVRGYETLPAFVK"
     gene            1286..1900
                     /locus_tag="VFX21_07340"
     CDS             1286..1900
                     /locus_tag="VFX21_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018500272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5095807.1"
                     /translation="MTDTRASGERRRYDSAVRRAQAAQTRERILSAAATLLRKSSIRN
                     WDGLTIRAVAERAGVHERTVYRHFANENALRDSVMQRLEAEAGVDLAHMRLDDVADVA
                     ASTIRHVSAYRMEPEPLTDPTLVAAKQRLHDALVAAVAEHTEAWTEADRTIAAAVLDV
                     LWAPGAYERLVANWQLNREEAIRAVTWAIELVRDAINAGRGPSG"
     gene            complement(1897..2409)
                     /locus_tag="VFX21_07345"
     CDS             complement(1897..2409)
                     /locus_tag="VFX21_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020123702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5095808.1"
                     /translation="MQPREIAEVLNHPLSKELLDQQLTRLSYVAEDGTPRVVPIAFVW
                     NDSEIVMTTAKNAPKLSALREHPAVALSIDTNDFPPKILLVRGRAKLDPVDGIPDEYL
                     QANNATKMSPEQREQWEIEIRSLYVDGMVRIVVTPTWVKLIDFDSTLPSAVQELMDQR
                     EARRRASTNR"
BASE COUNT          450 a          887 c          868 g          408 t
ORIGIN      
        1 tacaagcggc tgcgcgacga ggcgccgctc ttctacaacg aggagtacga cttctacgcg
       61 gtcagccgct acgacgacgt cgagcgcggg ctcgtcgacg ccaagcacca catctccgga
      121 cgcggcggca tcctcgaact gatcaaggcg gacatcgaga tcccgccggg caccttgatc
      181 ttcgaggatc cgccggtcca cacgatccac cggtcgctgt tgtcgcgcgt gttcacgccg
      241 cgccgcgtcg ccgcgctcga accgcagatc cgcgagtact gcgcgcgctg cctcgacccg
      301 ctcgaaggca aggacgagct cgacttcatc ctcgacttcg gcgcggagat gccgatgcgc
      361 gtgatcggca tgctgttcgg cattcccgag gaagaccagt tcaaggtcaa ggccagctcc
      421 gacgcgcggc tgcgcacgga ggccgggcag ccgatggacg tcggcgagaa cccgacgttc
      481 ggcagcgaga acttcgaggc ctacatcgac tggcggatcg accacccctc cgacgacctc
      541 atgaccgacc tcctcaccgc gcagtttcag gacgagaacg gcgagacccg cacgctgacg
      601 cgcgacgaag cggtcatgta cacgactgtt gtcgcgggcg cgggcaacga gaccacgacg
      661 aagctcatcg gctgggcggg caagacgctt gccgactacc ccgatcagcg ccgccaactc
      721 gtcgaggacc gctccctcat ccccaacgcg atcgaagaat tgctgcgctt cgagccgccg
      781 ggcccgcaca tcgggcgcta cgtgccggag cccgtcgagt ggtacgacac gacgatcccc
      841 gagggcagcg cgatcttgtt cgtcaacgcg tcggccaacc gcgacgagcg caagttcggc
      901 gacacagccg acagattcga catccaccgc gagatcaaga cgcacctcgc gttcggctac
      961 ggcattcact tctgcctcgg tgccgcgctc gcgcgactgc aaggacgcat cgcgctcgac
     1021 gagttgctca cgcgctggcc ggagtgggag gtcgacatcg cgcgcgccaa gatcgcgccg
     1081 acgtcgaccg tgcgcggcta cgaaacgctg cccgcgttcg tcaagtgatc cgtccggtga
     1141 cgttgtcgat gttcacggcg agtcaggggc gtcgctcgca aggacgcacg acgaaggaag
     1201 agtggggctc ttttgaggag gaggacgccg cgagcggcgt cactggccgg cgtgaacccg
     1261 gcaaacgttg ccggtcgggt cactagtgac ggacacgcgc gcctccggtg agcgccgccg
     1321 ctacgacagc gcggtgcggc gggcgcaggc ggcgcagacg cgtgaacgca tcctcagcgc
     1381 ggccgccacc ctgctgcgga agtcgtcgat ccggaactgg gacggcttga cgatccgggc
     1441 cgttgccgag cgcgcggggg tacacgagcg cacggtgtac cggcacttcg ccaacgagaa
     1501 cgcgctgcgc gactcggtga tgcaacgcct cgaggccgag gcgggcgtcg acctcgcgca
     1561 catgcgcctc gacgacgtcg ccgacgtcgc ggcgagcacg atccggcacg tctcggcgta
     1621 ccgcatggag ccggagccgc tgaccgaccc gacgttggtc gcggcgaagc agcgcttgca
     1681 tgatgcgttg gtcgcggccg tggccgagca cacggaggcc tggaccgagg ccgaccgcac
     1741 gatcgcggcc gcggtgttgg acgtgctgtg ggcgccgggc gcgtacgaac ggctcgttgc
     1801 aaactggcag ctcaaccgcg aagaggcgat ccgcgccgtc acctgggcta tcgaactcgt
     1861 ccgcgacgcc atcaacgcgg ggcgcggccc gtcgggctaa cggttcgtcg acgcgcgccg
     1921 gcgtgcctcg cgctggtcca tcagctcctg gaccgcgctc ggcagggtcg agtcgaagtc
     1981 gatcagcttg acccacgtcg gcgtgacgac gatgcgaacc atgccgtcca cgtagagcga
     2041 ccggatctcg atctcccact gctcgcgctg ttccggcgac atcttggtcg cgttgttcgc
     2101 ctggaggtac tcgtccggga tgccgtcgac cggatcgagc ttcgcccggc cgcgcacgag
     2161 caggatcttc ggcgggaagt cgttcgtgtc gatcgagagc gcgaccgcgg gatgctcacg
     2221 tagggcggac agcttcggcg cgttcttcgc cgtcgtcatc acgatctccg agtcgttcca
     2281 gacgaacgcg atcgggacga cacgcggcgt cccgtcctcg gcgacgtacg acagacgagt
     2341 gagctgctgg tcgaggagct ctttgctcag cggatggttc aggacttcgg cgatctcacg
     2401 gggctgcatg atcagacacc ctttccactg ttgttcgtcg ggtcgaagac ctctgaccta
     2461 cccggaacaa cgaactcatc gccgccgccg cctcgtcgtt tccgacattc gacccaacaa
     2521 ccgcgtcatg accgcgtaca tcacgttgcc gtacggccac tgggtgacga ctgtgccggt
     2581 cggtgcgcgg aagtagttgt tcgtcatggt cca
//
LOCUS       DASUGA010000372         2541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_223582, whole genome shotgun sequence.
ACCESSION   DASUGA010000372 DASUGA010000000
VERSION     DASUGA010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223582"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /gene="nuoB"
                     /locus_tag="VFX21_07350"
     CDS             complement(<1..373)
                     /gene="nuoB"
                     /locus_tag="VFX21_07350"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013016555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEX5095809.1"
                     /translation="MNLGLEAVPHNFLTGRLEDLVKWARRNSVWPATFGLACCAMEMM
                     ATGAAHYDTARFGMEVFRASPRQADLMIVAGRVSQKMAPVLRQVYDQMVEPKWVISMG
                     VCASTGGMFNNYALVQGVDQVV"
     gene            complement(373..849)
                     /gene="ndhC"
                     /locus_tag="VFX21_07355"
     CDS             complement(373..849)
                     /gene="ndhC"
                     /locus_tag="VFX21_07355"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX5095810.1"
                     /translation="MALDQYLPLFSMLALVFLFAALSFLASVLLGPKRPTSAKEAPYE
                     CGIVPEVEPADRFPVKFYLVAMAFIVLDVEIIFLYPFTTVLKGLAGYGLVVMGVFLLV
                     ALVPFGYLLSTGAVDWGPVRQVVERVGTRVLRAAGAPGRDGLDPDAPRPDSVEEAA"
     gene            1007..2257
                     /locus_tag="VFX21_07360"
     CDS             1007..2257
                     /locus_tag="VFX21_07360"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEX5095811.1"
                     /translation="MSATPIEPTATARPTSRTRKEAREHANRKRRRWVTIAVIGVLLL
                     ALIPIGKDYVDALRAPGTDAMGARTVEWMREHGFGPMVNSIERWWYTKNAPKVGGTLD
                     KLPKSQAVQSAAVGRIPSDMALLVGSVKETLLPKTPPPLKMAPFVDTPLPGEGEWSAT
                     GRSVLGTPGVYTAFMRPDPIHTSLVAGFMWMDPKLLKAVHVPGLKEPAGAPQKYGAQI
                     PNELRPSTVAAFNSAFRLDDARGGFWDEGNEVRPLVNGQASLVIDKDGSANVVAWGRD
                     RQLDQNVAQVRQNLALVVDNGQSAAGLDSEATTKWGATVGNQVLVWRSGIGVDKNGGL
                     IYIAGPGLSVNQLAELFLRAGAVRAMELDINSDWTTAYIFEQTDPNNPAAIFGDKLLP
                     DMKRNGDRYLVPGERDFVTMVARY"
BASE COUNT          441 a          870 c          876 g          354 t
ORIGIN      
        1 gcacgacctg gtcgacgcct tgcacgagcg cgtagttgtt gaacatgccg cccgtgctcg
       61 cgcacacacc catcgagatg acccacttcg gctcgaccat ctggtcgtag acctggcgga
      121 gcaccggcgc catcttctgg ctcacccgac cggcgacgat catgaggtcg gcctgacgcg
      181 ggctcgcgcg gaagacctcc atcccgaacc gggcggtgtc gtagtgcgca gcgccggtcg
      241 ccatcatctc cattgcgcag cacgccagac cgaaggtcgc cggccacacc gagttccggc
      301 gggcccactt cacgaggtcc tcgagtcgtc cggtcaggaa gttgtgcggg acggcctcca
      361 ggccgaggtt catcaggcgg cctcctccac cgagtcgggc cggggtgcgt ccgggtcgag
      421 tccgtcgcgg ccgggcgcac ccgcggcgcg gagaacgcgc gtacccacgc gctccaccac
      481 ttgacgcacg ggaccccagt cgacggcgcc ggtggagagg aggtagccga acggcacgag
      541 cgcgacgagc aggaagacgc ccatcacgac cagcccgtag cccgcgagcc ccttgaggac
      601 ggtggtgaag ggatagagga agatgatctc gacgtcgagg acgatgaagg ccatcgccac
      661 caggtagaac ttcaccggga agcgatcggc cggctcgacc tcgggcacga tgccgcactc
      721 gtacggcgct tccttcgcgg aggtgggccg cttcggaccc aagagcaccg acgcgaggaa
      781 gctcagcgcc gcgaacagga acaccagcgc cagcatcgag aacagcggca ggtactggtc
      841 gagagccacg aggcgcaggc tatccgaggc gcgcgggatc acggcaatac gacgaccgtg
      901 ctgtgcagtc ggatcgttgg cggcgcggag taccctgatc gggccttggg gtggctcccg
      961 cgagctcccg agttcgacgc ccgaatctcg aattggtgag cagctggtga gcgcaacgcc
     1021 catcgaaccg acggcgaccg ctcgccccac gtcgcgcacc cgcaaggaag cgcgcgagca
     1081 cgccaaccgc aagcgccgcc gctgggtcac gatcgcggtc atcggtgtgc tgctgctcgc
     1141 actgatcccg atcggcaagg actacgtcga cgcgctgcgc gcgcccggca ccgacgccat
     1201 gggcgcgcgc acggtggagt ggatgcgcga gcacgggttc ggcccgatgg tgaactccat
     1261 cgagcgttgg tggtacacga agaacgcgcc gaaggtgggc ggcacgctcg acaagctgcc
     1321 gaagtcgcaa gccgtgcagt cggccgcggt cgggcgcatc ccgtccgaca tggcgctgct
     1381 cgtcgggtcg gtgaaggaga cgctgctccc gaagactccg ccaccgctca agatggcgcc
     1441 gttcgtcgac acgccgctgc ccggtgaagg tgagtggagc gccacgggcc gctcggtgct
     1501 cggcacaccg ggcgtgtaca ccgcgttcat gcgacccgat ccgatccaca cgagcctcgt
     1561 cgcgggcttc atgtggatgg acccaaagct gttgaaggcc gtgcacgtgc ccggcttgaa
     1621 ggaacccgcc ggcgcgccgc agaagtacgg cgcgcagatc ccgaacgaac tgcgtccgtc
     1681 gacggtcgcc gcgttcaact ccgcgttccg gctcgacgac gcgcgcggcg gattctggga
     1741 cgagggcaac gaagtgcgtc cgctcgtgaa cgggcaggcg tcgctcgtga tcgacaagga
     1801 cggcagcgcg aacgtcgtgg cgtggggccg tgaccgtcag ctcgaccaga acgtcgcgca
     1861 ggtgcgccag aacctcgcgc tcgtggtcga caacgggcag tcggccgccg ggctcgactc
     1921 cgaggccacc acgaagtggg gcgcgaccgt cggtaaccag gtgctcgtct ggcgctcggg
     1981 catcggcgtc gacaagaacg gtggcctcat ctacatcgcc ggccccggcc tgtcggtgaa
     2041 ccagctcgcc gagctgttcc tgcgcgccgg ggcagtgcgc gcgatggagc tcgacatcaa
     2101 ctccgactgg acgaccgcgt acatcttcga gcagaccgac ccgaacaacc cggccgcgat
     2161 cttcggcgac aagctgctgc ccgacatgaa gcgcaacggc gaccgctacc tcgtgcccgg
     2221 cgagcgcgac ttcgtcacca tggtggcgcg ctactagctc gctcccgcgg tcgcacggga
     2281 ccgcgtagcc ccgaagcgtc gcgcgcgtct gcgcagtcca gcgattgcca ttgcgaaggc
     2341 agggggccac gccgaggacg gaggcgtgcg cgcggcgctt gtgctagggc tcgggcgtga
     2401 cgcgcgcgat ggtggcgacc atcttgtaga cgacttccgc gccgccgcgt tcgtcgggcc
     2461 gcatcaagtt cgccatgatg cgcaacaccc actccatgag cgtcttcgac tggaagccga
     2521 tgcgcgtgag ctcgcgcatc a
//
LOCUS       DASUGA010000373         2367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1889907, whole genome shotgun sequence.
ACCESSION   DASUGA010000373 DASUGA010000000
VERSION     DASUGA010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889907"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1576
                     /locus_tag="VFX21_07365"
     CDS             <1..1576
                     /locus_tag="VFX21_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5095812.1"
                     /translation="QVQHPLIVEIYNFASHEGAGYIVMEYVGGRSLKQFLKERMAANG
                     GKYTPFPVDQAIAYIVEILPAFAYLHSQGLLYCDFKPDNMIQTGDALKLIDLGGVRRG
                     DDNVSAIYGTVGFQAPEVAEVGPSVASDIFTIGRTLTVLAMEFRGYQSTYVASLPPAE
                     DAPLFQRYDSLYRTIQKATAPNPDDRFQTADELRSQLIGVLREVVAVDSGKAAADHSS
                     PSDLFGAPTATGAELAWTDLPLLRIDHTDPAATWLAGVSLGAPDQRLLVLQQAPQQTV
                     EVQLAKARAAIEAGMYDDANVLLDGTLADNPWEWRAIWLQGLAALAKNDAQSALTAFN
                     TVLSQVPGELAPKLALALACEHGGVADVAEYLYAVCAATDANYVAPAAFGLTRTREMR
                     QDVTGAIASLDLVAPTSGAYIAARRRRAELLTAPGRGLNDLALAAASIEDLAIDPRDR
                     QVALVAILSAALAEVKKHGDQPAIQVARNVPATELSLRAAAEHAYRELASLTPDRAMR
                     VMLVDKANEIRPRTMV"
     gene            1587..>2367
                     /locus_tag="VFX21_07370"
     CDS             1587..>2367
                     /locus_tag="VFX21_07370"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX5095813.1"
                     /translation="MKCPHCGEEVAETDQFCESCGQSLSTAPQPVAVLATEGGSAELG
                     TQLLAPPGAGDAAPAGPTCSCGGLIDDDGFCTVCGLRAPSERDHFSEQPAAGIAAVCD
                     KGIVHRRNEDAVAVGATGTRLIAVVCDGVTNATDSDIVSLAAARAARDVLQNAPVETG
                     DTVEHWRVQHNAAAAAAQDAAMNAAQDAHLARAENPPSCTYVAAIADGATLAAAWIGD
                     SRAYWFGDDGAATQLSVDDSWATAEIERGVDRATAEADPRAH"
BASE COUNT          402 a          807 c          801 g          357 t
ORIGIN      
        1 ccaggtgcag cacccattga tcgtcgagat ttacaacttc gcgagtcacg agggcgcggg
       61 ctacatcgtc atggagtacg tcggcgggcg ctccttgaag cagttcctga aagaacgcat
      121 ggctgcgaac ggcgggaagt acacgccgtt cccggtcgac caagcgatcg cgtacatcgt
      181 cgagatcctc cctgcgtttg cgtacttgca ttcgcagggt ctgctctact gcgacttcaa
      241 gcccgacaac atgatccaga ccggtgatgc gctcaagctc atcgacctcg gtggcgtgcg
      301 ccgcggcgac gacaacgtca gtgcgatcta cggcaccgtc ggattccaag cgcccgaggt
      361 cgcggaggtc gggccgtcag tagcgagcga cattttcacg atcggccgca cgctcaccgt
      421 gctggcgatg gagttccgcg gctaccagag cacgtatgtc gcgtcgctgc cgccggcaga
      481 ggacgcaccg ttgttccagc gctatgactc gctctaccgc acgatccaaa aagcaaccgc
      541 gccgaatccc gacgatcgct tccagaccgc cgacgaactg cgcagccagc tgatcggcgt
      601 gttgcgcgag gtcgttgccg tcgattcagg caaggcggcg gccgaccact ccagtccgtc
      661 cgatctgttc ggggcgccaa ccgcgaccgg cgcggagttg gcgtggactg atctgccgtt
      721 gttgcgtatc gaccacaccg atcccgctgc aacctggctc gccggcgtct cgctcggggc
      781 tcccgaccag cgcctgctcg tcttgcaaca ggcaccccaa caaacagtcg aagtccagct
      841 cgccaaagcg cgcgccgcga tcgaagcggg catgtacgac gacgccaatg tgctcctcga
      901 cggaacgctc gccgacaatc cgtgggagtg gcgggcgatc tggctgcaag gcctcgcggc
      961 gctggcaaag aacgatgccc agtcggcgtt gaccgcgttc aacacggtgc tcagccaggt
     1021 accgggcgag ctggctccga agctcgcgct ggcgctggcg tgtgaacacg gcggcgttgc
     1081 cgacgtggcc gagtacctct atgcggtgtg cgcggcgacc gacgccaact acgtcgcgcc
     1141 cgccgcgttc gggctcactc gcacacgcga gatgcgccag gacgtgaccg gcgcgatcgc
     1201 ctcgctcgat ctggtggcac cgacgagcgg cgcgtacatc gcggcccggc ggcggcgcgc
     1261 ggagctgctc acggcaccgg gacgcgggtt gaacgacctc gcgctggccg cggcgagcat
     1321 cgaagacctt gcgatcgatc cgcgtgaccg gcaggtcgcg ctggtcgcga tcctcagcgc
     1381 cgcgctggcc gaagtgaaga agcacggcga tcagcccgcg atccaggttg cccgcaatgt
     1441 gcctgccacc gagttgagcc tgcgcgccgc cgcggaacac gcataccgcg agttggcgtc
     1501 gctgacgccc gaccgggcaa tgcgcgtgat gctcgtcgac aaggctaacg aaatccggcc
     1561 gcggacgatg gtgtgaggcg ccgcgcatga agtgtccgca ctgcggagag gaagtcgccg
     1621 aaaccgacca gttctgcgaa tcgtgcggcc agtcgctgtc gactgcgccg caaccggtcg
     1681 cagtgctcgc gaccgaaggc ggcagtgccg agctcggaac gcagttgctg gccccacccg
     1741 gtgccggcga cgcggcgccg gccggaccca cgtgctcgtg tggtggcctc atcgacgacg
     1801 acgggttctg tacggtgtgc ggactccgtg cgccgtcgga acgcgatcac ttctcggaac
     1861 agccggcggc gggcatcgcc gcggtgtgcg acaaaggcat cgtgcatcgc cggaacgaag
     1921 atgccgtcgc ggttggcgcg accggcacgc gcttgatcgc cgtcgtgtgc gacggtgtga
     1981 cgaatgcaac cgacagcgac atcgtgtcgc ttgcggccgc gcgcgccgcg cgcgacgtgc
     2041 tccagaacgc gcccgtcgaa accggtgaca ccgtcgagca ctggcgcgtc cagcacaacg
     2101 cggcggcggc cgccgcgcag gacgcggcca tgaacgcggc ccaggacgca catctggcgc
     2161 gagcggagaa tcctccgtcc tgcacctacg ttgcggcgat cgccgatggt gccacgctcg
     2221 ccgcggcttg gatcggcgac agccgcgcct actggttcgg tgacgatggc gcggcgacgc
     2281 agctatcggt cgacgactcg tgggcgacgg ccgaaatcga gcggggcgtc gaccgcgcca
     2341 ccgccgaagc cgacccgcgt gcgcact
//
LOCUS       DASUGA010000374         2461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_152461, whole genome shotgun sequence.
ACCESSION   DASUGA010000374 DASUGA010000000
VERSION     DASUGA010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152461"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1636
                     /locus_tag="VFX21_07375"
     CDS             <1..1636
                     /locus_tag="VFX21_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095814.1"
                     /translation="LAVEHDPAAVADAVRQLFATRGTEAVAPALQGAYNFVGANEQWG
                     LRAFTSFSGLFPLYWQQTDGVTVVSNRSTTVRAVLDNAGWSPSALAWVIGHANLFFDE
                     MPAAGVSYLRPGMELRVNLADGRSTLVRAHDWIWPAPSEDPARADLSDDEWDEITQAL
                     IANFRVLGSYDARLMLSGGKDSRLCLALAKAAGLADRLRTVTTGPPNNAEALCAAEVA
                     RRAGFEHKHAGQVTETGPKVVDDDAMRTAVLNARQWRLYRQHVHRYEAIVCPADGLTP
                     EARDTVLSIKGFGGELYRGPGGHAKKFGKTDVESLQQMADMFVNYHQPHDPLGVLVPD
                     VATAQAEQLRAWVYDTAAEIRFDLLPEKFFVDYRLGHWNGPLGQYTPGDIKINPLLSA
                     RAAQLCLSLSKHARSMDLLHFQVMRRTAPELLGVPIVNQAWHPALQELSPVLLPAEPW
                     PAEVRVNARTLKPRRWAFLTEERDAVRALFEDAHETQMREIVDFGALLASIDDPASLS
                     NVATKEILSNIAVALILLRRGVPVLDRIAPEPESVT"
     gene            complement(1629..>2461)
                     /locus_tag="VFX21_07380"
     CDS             complement(1629..>2461)
                     /locus_tag="VFX21_07380"
                     /inference="COORDINATES: protein motif:HMM:NF021850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative capsular polysaccharide synthesis
                     family protein"
                     /protein_id="HEX5095815.1"
                     /translation="SDGSHMRLVNDLRYNLEKAHAMRRLGTPSGRPVVVFTMDQSGAP
                     VAHALRARLDRPVFHVHRLNYGSVSAAEGRCRRTEPTARPHDLWASEFLLRRLPTRDD
                     PWDVVTIVREPVAQAVVAQFPAADRTIDLTRTSLEVLTRTFEDQTDFRNPLRWFDREF
                     EPGLDLCVYDYDFDPVQAHGIIETPEVRVLILRSENLDAAPAALAEFFGQLDPVIIRT
                     DNVARHQMRADISRELAGDELLPRSLLDRAYNSRYTKHFYSADEIARFRRTWEHNRVT
                     "
BASE COUNT          409 a          793 c          833 g          426 t
ORIGIN      
        1 gctcgccgtc gagcacgatc cggcggccgt ggccgacgct gtgcggcagt tgttcgcgac
       61 gcgcggcacc gaagcggtcg cgccggcgct gcaaggggcg tacaactttg tcggcgcgaa
      121 cgagcaatgg ggtctccgcg ccttcacgag cttctccggc ctgttcccgc tctactggca
      181 acagacggat ggcgtcacgg tggtgtccaa ccgatcgacg acggtgcgcg ccgtcctcga
      241 caatgccggt tggagcccga gcgctctggc gtgggtcatc gggcacgcga atctcttctt
      301 cgacgagatg cccgcggccg gcgtttcgta cctgcggccc ggcatggagc tgcgcgtcaa
      361 cctcgcggac ggtcgatcca cactggttcg cgcgcacgat tggatctggc ccgcgccgtc
      421 cgaggatccg gcccgtgccg atctcagcga cgacgagtgg gacgagatca cccaggcatt
      481 gatcgcgaac ttccgcgtcc tgggttcgta cgacgcgcgg ttgatgctct cgggcggaaa
      541 agattcgcga ctctgtctcg cgctggccaa ggccgccggc ttggcggacc gtctgcggac
      601 cgtcaccacg gggccgccca acaacgcgga agcactgtgc gccgcggaag tcgcgagacg
      661 cgcgggcttc gagcacaaac atgccggtca ggtgaccgag accggcccga aagtcgtcga
      721 cgacgatgcc atgcgtactg cggtcttgaa cgcgcggcag tggcgcttgt atcgccaaca
      781 cgtacatcgc tacgaggcca tcgtgtgtcc tgcagacggc ttgacgcccg aagctcggga
      841 tacggtcttg agcatcaagg gtttcggcgg tgagctctac cggggcccgg gtggtcacgc
      901 aaagaagttc ggcaagacgg acgtcgagtc gctccagcag atggccgaca tgttcgtcaa
      961 ctatcaccaa cctcacgacc ctctcggcgt cctggtcccc gacgtcgcga ccgcgcaggc
     1021 tgaacagctc cgcgcctggg tgtacgacac tgcggccgag atcaggttcg atctccttcc
     1081 ggagaagttc ttcgtcgact accggctcgg acattggaac ggtccgctcg gtcagtacac
     1141 gccgggagac atcaagatca acccgttgct ctcggcccga gccgcgcagc tctgcctctc
     1201 gctctcgaag cacgcgcgat cgatggatct cttgcatttc caggtgatgc ggcgaacggc
     1261 gccggagctg ctcggcgtgc cgatcgtcaa ccaggcgtgg catccggcgc tccaggagct
     1321 ctcaccggtc ctcctgcccg ccgaaccctg gccggcggag gtcagggtga acgctcgtac
     1381 cttgaagccg cggagatggg cgttcttgac cgaagagcgt gacgcggttc gcgcgttgtt
     1441 cgaggacgcc cacgagacgc agatgcgcga gatcgtcgac ttcggcgcgc tgctcgcgag
     1501 catcgacgat ccggcgtcgt tgagcaatgt cgcgacgaag gagatactca gcaacatcgc
     1561 cgtggcgttg attctgctgc gacgaggtgt gcccgtgctc gaccggatcg ctcccgaacc
     1621 ggagtcggtc acgtgacgcg gttgtgctcc caggtccgac ggaagcgagc gatctcgtcg
     1681 gcggagtaga agtgcttcgt gtagcgcgaa ttgtacgccc ggtcgaggag cgaccgcggt
     1741 aacagttcat cgcccgcaag ctcgcgcgag atgtccgcgc gcatttggtg cctcgccacg
     1801 ttgtcggtgc ggatgatcac ggggtccaac tgcccgaaga actcggcgag tgctgcgggc
     1861 gcagcatcga ggttctcgct gcgcaggatc agcacgcgca cctcgggtgt ctcgatgatg
     1921 ccgtgcgcct gcaccggatc gaagtcgtag tcgtacacgc aaagatcgag gccaggttcg
     1981 aactcgcggt cgaaccaacg cagcgggttc cggaagtcgg tctggtcctc gaacgtgcgc
     2041 gtcaagactt cgagggatgt cctcgtcagg tcgatcgttc ggtccgcggc cgggaactgg
     2101 gccacgactg cctgcgcgac cggttcgcgc acgatcgtga cgacgtccca tgggtcgtcc
     2161 cgcgtcggca gtcgtcgcag gaggaactcg gacgcccaga gatcgtgggg ccgggcagtt
     2221 ggttcggtgc ggcggcagcg gccctcggcc gcgctgaccg agccgtagtt gagccggtgg
     2281 acgtggaaca ccggccgatc gagccgggcc cggagcgcgt gcgccaccgg agcacccgat
     2341 tggtccatcg tgaacacgac gacaggacgg cccgacggcg tgccgagacg ccgcatcgcg
     2401 tgcgccttct cgaggttgta gcgcaggtcg ttgacgagcc tcatatgcga tccatcgctc
     2461 t
//
LOCUS       DASUGA010000375         2565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1251210, whole genome shotgun sequence.
ACCESSION   DASUGA010000375 DASUGA010000000
VERSION     DASUGA010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251210"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VFX21_07385"
     CDS             <1..732
                     /locus_tag="VFX21_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5095816.1"
                     /translation="VTGGSRGIGLMIARGFVEGGAKVYISSRKAEVCDEVAAELSKSG
                     ECISLPADLATEQACNDLAAGIASREDKVHILVNNAGATWGAPIADFPASAWDKVLDL
                     NVKSPFFLTRALLPHLEAAGTADDPARVINIGSIDGIQVPMMETFSYSSSKAAVHQLT
                     RHLARHLGRKHITVNAIAPGPFESKMMAETLRNFGDSIAASSPLNRIGRPDDMAGTAI
                     FLASRAGAYVTGAVIPVDGGIATTK"
     gene            752..1945
                     /locus_tag="VFX21_07390"
     CDS             752..1945
                     /locus_tag="VFX21_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5095817.1"
                     /translation="MADDLTRLDARIDELLAACDPRDMKTQEFRGEQYDRGLAWVHFP
                     DGFGGMGLPPGLQRHVDQRLRAAGAKAPHGTLFFGLSLAGPTVVTHGSDDLRNRALRP
                     MFTGEEIWCQLFSEPGSGSDLAGLSTRAIRDGDEWVVNGQKVWNTLAHLADRGMLVAR
                     TDPELPKHKGLSYFMVDMHAPGVDVRPLRQITGEAEFNEVYLTDVRIADRDRIGDIGD
                     GWRVSMTTLMNERTTIGGATSSAPRRATPVAEALDIWSKREDKDPVLRDRIAKLYIDF
                     EVLRILNARGAANRKAGNPGPEGSIAKLQLATLNKAAYELCIDLLGPAGVVGYDYTFK
                     RPDEAGLDAPLGSARKMFLRARANSIEGGTSEIMRNILGERILGLPGEPRVDRDLPWS
                     QVPRN"
     gene            1948..2307
                     /locus_tag="VFX21_07395"
     CDS             1948..2307
                     /locus_tag="VFX21_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5095818.1"
                     /translation="MDRAAAQAWVDAYERAWRTAGTEPVRALFAEDATYSMGPYEAVE
                     RGIAAIEALWERERVSHDEPFTMTSEIVAAEGDVAVARLEVHYQATGNEFRDLWVMRF
                     AADGRCVAFEEWPFAPE"
     gene            2351..>2565
                     /locus_tag="VFX21_07400"
     CDS             2351..>2565
                     /locus_tag="VFX21_07400"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5095819.1"
                     /translation="MSQLGVDGFRAERDTILTIAKSCTDDEWNAQSDCTGWAVRDVLA
                     HLAATLHGVVDPTFMPDMSGGTENAMEA"
BASE COUNT          434 a          878 c          889 g          364 t
ORIGIN      
        1 gtcaccggcg gcagccgcgg catcggtctc atgatcgccc gcggcttcgt cgagggcggg
       61 gcgaaggtct acatctcgtc gcgcaaggcc gaggtctgcg acgaggtcgc ggccgagctg
      121 tcgaagtccg gcgagtgcat cagcctgccc gccgaccttg ccaccgagca ggcgtgcaac
      181 gacctcgcgg ccgggatcgc gtcgcgagag gacaaggtgc acatcctcgt caacaacgcg
      241 ggcgcgacat ggggcgcgcc gatcgccgac ttccccgcgt cggcgtggga caaggtgctc
      301 gacctgaacg tgaagtcgcc gttcttcctg acgcgcgcgc tcctgccgca cttggaagcg
      361 gccggcaccg ccgacgatcc ggcgcgcgtc atcaacatcg ggtcgatcga cgggatccaa
      421 gtgccgatga tggagacgtt ctcctactca tcgagcaaag ccgcggtgca tcagctgaca
      481 cggcatctcg cgcggcacct cggtcgcaag cacatcaccg tgaacgcgat cgcgcccgga
      541 ccgttcgagt cgaagatgat ggcggagaca ctgcgcaact tcggcgattc gatcgcggcg
      601 tcgtcaccgc tcaaccgcat cggtcggccg gacgacatgg ccggcactgc gatcttcctt
      661 gccagccggg cgggtgcgta cgtcaccggt gcggtgatcc cggtcgacgg cggcatcgcg
      721 acgacgaagt agtgggggtt agtgtgcggc catggccgat gacctgacgc gcctcgacgc
      781 ccgcatcgac gagctcctcg ccgcgtgtga tccacgcgac atgaagacgc aggagttccg
      841 cggcgagcaa tacgaccgcg ggctcgcgtg ggtgcacttc ccggacggat ttgggggcat
      901 gggactcccg cccggattgc agcggcacgt cgatcaacgg ctgcgcgccg ccggcgccaa
      961 ggcgccgcac ggcacactgt tcttcggcct ctccctcgcg ggaccgaccg tcgtcacgca
     1021 cggcagcgac gatctgcgca accgcgcgct gcgcccgatg ttcacgggcg aggagatctg
     1081 gtgccagctc ttctcggagc cgggttcggg ctccgacctc gccgggctct ccacgcgcgc
     1141 gatccgcgac ggcgacgagt gggtggtcaa cggccagaag gtgtggaaca cgctggccca
     1201 cctcgccgac cgcgggatgc tcgtcgcccg caccgacccc gagctgccga agcacaaggg
     1261 cctcagctac ttcatggtcg acatgcacgc gcccggtgtc gacgtgcgcc cgctgcggca
     1321 gatcaccggg gaggcggagt tcaacgaggt gtatctcacc gacgtgcgta tcgcggaccg
     1381 cgatcgcatc ggtgacatcg gcgacggctg gcgcgtctcc atgacgacgc tgatgaacga
     1441 gcgcacgacc atcggtggtg cgacgagctc cgcgccgcgg cgggcgacgc cggtcgcgga
     1501 agcgctcgac atctggtcga agcgggaaga caaggacccg gtgctgcgcg accgcatcgc
     1561 gaagctgtac atcgacttcg aggtgctccg gatcctcaac gcgcgcggcg ccgcgaaccg
     1621 caaggccggc aacccgggcc ccgaaggctc gatcgccaag ctgcagctcg cgaccttgaa
     1681 caaggctgcg tacgagctgt gcatcgacct gctcggaccg gccggcgtcg tcggctacga
     1741 ctacacgttc aagcgtcccg acgaagccgg cctcgacgcg ccactcggct cggcccgcaa
     1801 gatgttcctg cgggcacggg cgaactcgat cgaggggggc acgtccgaga tcatgcgcaa
     1861 catcctcggc gagcggatcc tcggcctgcc cggcgagccg cgcgtcgacc gcgacttgcc
     1921 gtggtcgcaa gtgccgcgca actagtggtg gatcgcgcgg cggcgcaggc gtgggtcgac
     1981 gcgtacgagc gcgcgtggcg caccgcgggc accgaacccg tgcgcgcgct gttcgccgag
     2041 gacgcgacct actcgatggg tccgtacgaa gcggtcgagc gcggcatcgc cgcgatcgaa
     2101 gcgctgtggg aacgcgagcg cgtcagccac gacgaaccgt tcaccatgac gtccgagatc
     2161 gtcgccgcgg agggcgatgt cgcggtcgcc cgtctcgaag tgcactacca ggcgaccggc
     2221 aacgagttcc gagacctgtg ggtcatgcgc ttcgccgccg acgggcgctg cgtggcgttc
     2281 gaagaatggc ctttcgcccc cgaatgaccg catcggcaag gatgccgagg acacaacgga
     2341 ggagcacacc atgagccagc tgggagtcga cggtttccgc gccgagcgcg acaccatcct
     2401 cacgatcgcg aagagctgca ccgacgacga gtggaacgcg caatcggact gcacgggttg
     2461 ggccgtgcgt gacgtgctcg cgcacctggc cgcgacgctg cacggcgtcg tcgacccgac
     2521 gttcatgccc gacatgagcg gcggcaccga gaacgcgatg gaggc
//
LOCUS       DASUGA010000376         7404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_568080, whole genome shotgun sequence.
ACCESSION   DASUGA010000376 DASUGA010000000
VERSION     DASUGA010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7404
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568080"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFX21_07405"
     CDS             <1..528
                     /locus_tag="VFX21_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003079591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5095820.1"
                     /translation="NAIGAVLPEGVIVVDEAQTGGLFVSGATAGAPRHDWLTLTGGAI
                     GLGLPMATGAAVACPDRKVLCLEADGSAMYTLQALWTQAREQLDVTTLLFDNRSYAIL
                     NIELGRVGAGTGGPRAQSLLDLTNPTLDFVQLARGMGVDATRPETAEDLTNELERALS
                     TPGPHLIDAVLPSRL"
     gene            complement(492..944)
                     /locus_tag="VFX21_07410"
     CDS             complement(492..944)
                     /locus_tag="VFX21_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095821.1"
                     /translation="MTRWLFWLAASGAVAGAVAASLAVRAWIRFEDGVDAGGLFLSFG
                     SYQSARDDLDLALAACTLLGIALLVCLGVGAVQRVPWPALRWAALVLAVELLLRIGVV
                     WADGSTAREIRRGYAFITASTLGFLATGVALALAARGYNREGSTASMR"
     gene            complement(941..1954)
                     /locus_tag="VFX21_07415"
     CDS             complement(941..1954)
                     /locus_tag="VFX21_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEX5095822.1"
                     /translation="MTAIVREATAPPEARGITRDAVRMMVADKSNLEIVHTYAHDLPR
                     FLDEGDLVVVNTSGTLAAALDALGEDGTPLVVHLSQRLPAEAWLVELRRPDNGSTKPW
                     FDDPPGRELRLVGGGTVALASRLPGSQRLWLATLSLPDPLLTYLTKHGRPIRYGYVTQ
                     DWPLDMYQNVYSREPGSAEMPSAGRPFTPELITRLIAKGVDVAPLVLHTGVSSLEANE
                     QPYPEWYRVAPSTAARVNRTHDEGGRVIAIGTTVVRALETVSDKRGVVHPGEGWTDLV
                     VTPERGVHTIDGMLTGWHEPEASHLMMLEAVVGRPVLEVAYAAAEADGYLWHEFGDVN
                     LLL"
     gene            complement(1951..2637)
                     /locus_tag="VFX21_07420"
     CDS             complement(1951..2637)
                     /locus_tag="VFX21_07420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5095823.1"
                     /translation="MQVALVTGASRGFGLALARRLARDGYALVLDARGAPALDDAARE
                     LAAITTVRAIPGDVADPEHRAALIAAAWELGGLDLLVNNASVLGPSPQPALAEYPIDT
                     LRAVYEVNVLAPIALTQEALPLLRARHGTIVNVTSDAAVEPYEGWSGYGSSKAALEQA
                     GNVLGAEEHDVRVYSFDPGDMRTQMHQDAFPGEDISDRPLPESVVPSLIALLQEYPVS
                     GRYRAQELVP"
     gene            2744..3781
                     /locus_tag="VFX21_07425"
     CDS             2744..3781
                     /locus_tag="VFX21_07425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007694096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5095824.1"
                     /translation="MTGFDVAVVGGGLVGAATAYELAIGGARTVVYDRADAGRATGAG
                     AGILSPETAVRDDAAWTELCRAAGAHYDALVPQLGGDTGWARCGILELATRESDVGAF
                     EWTAARASGAREIDPDDARGMCPVLGDVQRALHHPDAARVDGRMMAAALQHAAIARGV
                     DWRAGAAPPVTEIGAEAVVIAGGAWTPETSAQVGVALPITPLRGQIMHLDLPEHDTAS
                     WPIVQPVFGHYMVPWADRHVAVGATVEDAGFAAEVTAGGVQEILREALRVMPGLAPAR
                     LREVRVGLRPCSADDLPVIGPLVGNVFVCTGHGANGLLLGPVSGRLVADCVLSRESQV
                     DLAPFTPARFV"
     gene            3789..4946
                     /locus_tag="VFX21_07430"
     CDS             3789..4946
                     /locus_tag="VFX21_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095825.1"
                     /translation="MPATGGAGMGVRGSFGMLLCCFVALAGCGGDDDSAASDDDVTTT
                     SNEVTTSTIAAVPTLDGLYFLTGTQTCKSALGPGTTSFPLFAGSTLAISGDRAWAGLR
                     SDPPEASVTTDGASFHVNVTYSGRTNAAVTTVAGASPSALDLVLDLSGTISDDGQTIT
                     GTGFDTGVNCEYEFTGSRSAIGDTGAGTEKCGGEAALVKVVAETPREDGLTFTADDVI
                     VEAMTPDGQIARYLIGQAAEGFAGCEPDGWKLADPDIVRLALCESELGKEIAPQLDVD
                     CGPSTTAPSAAPTTTPVVTAPANPVSGSCDAQGALDATIAKHPDTIARDLDTVLACED
                     GWAVVVWSTGDADLMEVLQLRDGAWVATPGAACTGAAGIPESVRFYACMTG"
     gene            complement(4954..6159)
                     /locus_tag="VFX21_07435"
     CDS             complement(4954..6159)
                     /locus_tag="VFX21_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5095826.1"
                     /translation="MDFAWEAEDLAFKEELEAFLDKELPPFLDEWTDDDLEPSRGVMG
                     VMDKRKAWQHKLNEGRWSAILWPEEWGGRAATTSQQVIYTQVMSKYRTPGIYNANGIV
                     QIGPSIIAWGTEEQKSKWLPGILDASEHWCQGFSEPQAGSDLANLRTTAILSDDGTHY
                     VVNGQKIWISSAQIAKWGLFLFRTDPTAISRGKKHEGITAFIVDMELPGIDIRPIREI
                     TGDSMFCEVFFTDVKVPVGDRLGDENLGWMVSMGALGQERVGSSGQAISMAQDLRRLL
                     QTAKAENPQALEDPAIRERVARLHTQIEYTRLLIARALSKVLKGEKGWPEVPLAKLQW
                     GYISLWLAELALDILGPTGTLLKGAPGAIDGGVWARNYVWQRYTTIGAGPTEVQKNII
                     ADRALKLER"
     gene            6185..7282
                     /locus_tag="VFX21_07440"
     CDS             6185..7282
                     /locus_tag="VFX21_07440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5095827.1"
                     /translation="MDFTFSADQDALRDTVRSFMRAESPKEYVRRMSEHDDIGVEPAV
                     WRKLVDLGWTALLVPEEQGGLGLGVVDAVVVQEEMGRATFPGPYFSSAILATLAARAL
                     GLDEQLAALAAGAQRGAVALEEQGWGDPVERARVRANGRGARFKLDGTKPFVMDGHSA
                     DWVLVTARTREGLQTFLVEKPAAPVVSSLDITRKFARLEFEDTPATLVGPPGDHTAIW
                     HRVADDAAVLLAAELIGVCEAATELALDYAQAREVFGKPLSKFQVTRHKAVDMLHQIE
                     LAKVGVHYAAWASDVDAPDRESAAAMAKSYAAEAANYIGAECIQIHGGIGFTWENDAH
                     IYLRRAKVDDLLLGDQNWQRGRVADLYFAGL"
BASE COUNT         1114 a         2514 c         2632 g         1144 t
ORIGIN      
        1 aacgcgatcg gcgcggtact gcccgaaggc gtgatcgtcg tcgacgaagc acagaccggc
       61 gggctgttcg tctcgggtgc gactgccggc gcgcctcgtc acgactggct gacgctcacc
      121 ggcggcgcga tcggcctcgg actgccgatg gcgaccggcg cggccgtcgc gtgccccgac
      181 cgcaaggtgc tgtgcctcga agccgacggc agcgcgatgt acacgctgca ggcgctgtgg
      241 acgcaggcgc gcgagcaact cgacgtgacg accctcctgt tcgacaaccg ctcgtacgcg
      301 atcttgaaca tcgagctcgg ccgggtcggt gccggcacgg gcgggccgcg cgcccagtcg
      361 ctgctcgacc tgacgaaccc gacgctcgac ttcgtgcagc tcgcgcgcgg catgggcgtc
      421 gacgcgacgc ggccggagac ggccgaagac ctgacgaacg agttggagcg ggcgctgtcg
      481 acgcccggcc ctcacctcat cgacgcggtg ctgccctcgc ggttgtagcc gcgcgccgcg
      541 agcgcgagcg cgacgcccgt ggccaggaac ccgagtgtgc tcgcggtgat gaacgcgtag
      601 ccgcgccgga tctcgcgtgc cgtcgagccg tcggcccaga cgacaccgat gcggaggagc
      661 aactccacgg ccagcacgag cgcggcccag cgcagcgccg gccacggcac tcgctgaacc
      721 gcgcccacgc cgaggcacac gagcagcgcg ataccgagga gtgtgcacgc agccaacgcg
      781 agatcgaggt cgtcgcgcgc cgactggtac gacccgaacg acaggaagag cccgcccgcg
      841 tcgacgccgt cctcgaaccg gatccatgcc cgcaccgcca gagacgccgc gaccgctccc
      901 gcgacggcgc ccgacgccgc cagccagaac agccaccgcg tcacaggagc aaatttacgt
      961 ctccgaactc gtgccagagg tagccgtcgg cttcggcggc cgcgtacgcg acttcgagga
     1021 ccggccggcc gaccactgct tccagcatca tcaagtgtga tgcttccggt tcgtgccagc
     1081 cggtgagcat gccgtcgatc gtgtgcacgc cgcgttcggg cgtgacgacc aggtcggtcc
     1141 agccttcacc gggatggacg acgccgcgct tgtcactcac ggtctcgagg gcgcgcacga
     1201 cggtcgtgcc gatcgcgatc acgcgtccgc cttcgtcgtg ggtgcggttg acgcgcgcgg
     1261 cggtcgacgg tgcgacgcga taccactcgg gatagggctg ctcgttggct tcgagtgacg
     1321 acacgcccgt gtgcaacacc aacggcgcga cgtcgacgcc ctttgcgatc aagcgcgtga
     1381 tcaactcggg cgtgaatggg cggcccgcgc tcggcatctc cgcactgccc ggttcgcgcg
     1441 agtacacgtt ctggtacatg tcgagcggcc agtcctgcgt cacgtacccg tagcgaattg
     1501 ggcggccgtg cttcgtcagg tacgtcagca acgggtcggg cagcgagagt gtcgccaacc
     1561 acaaccgttg cgatcccggc agccgtgacg cgagcgcgac ggtcccgccg ccgacgagtc
     1621 gcaactcgcg accgggcgga tcgtcgaacc agggcttggt cgatccgttg tcaggacgac
     1681 gcaactcgac gagccaggct tcggccggca aacgctgtga caggtgcacg accaggggcg
     1741 tgccgtcctc gccgagcgca tccagtgcgg ccgccagcgt gcccgaggtg ttgacgacga
     1801 cgaggtcgcc ttcgtcgagg aaccgaggca ggtcgtgcgc atatgtgtgg acgatctcga
     1861 gattggactt gtcggcgacc atcatgcgca ccgcgtcgcg ggtgatgccg cgtgcctcgg
     1921 gcggcgcagt cgcttcccgc acgatcgcgg tcacgggacc agttcctgcg cgcggtaccg
     1981 gccgctcacc gggtactcct gcaacagcgc gatgagtgac ggcacgacgg actccggcaa
     2041 cggacgatcg gagatgtcct caccggggaa cgcgtcttga tgcatctgcg tgcgcatgtc
     2101 gccggggtcg aagctgtaca cgcggacgtc gtgctcttcc gcgcccaaca cgttgccggc
     2161 ttgttcgagc gcggccttcg acgatccgta gccgctccac ccttcgtacg gctccacggc
     2221 ggcatccgac gtcacgttga cgatcgttcc gtgtctcgcg cgcaacaggg ggagcgcctc
     2281 ttgcgtcagc gcgatcggtg cgagcacgtt cacctcgtag actgcgcgca gcgtgtcgat
     2341 cgggtactcg gcgagcgccg gttggggcga cgggccgagc acgctcgcgt tgttgacgag
     2401 caggtcgagt ccgcccagct cccacgcggc cgcgatgagc gcggcgcggt gctcggggtc
     2461 ggcgacgtcg ccggggatcg cccgcacggt ggtgatggcg gcgagctcac gggcggcatc
     2521 gtcgagggcg ggagcgccgc gggcgtcgag gacgagtgcg tagccgtccc gggcgaggcg
     2581 gcgggccagg gccagcccga agccgcggga ggcgccggtg accagtgcaa cctgcatggt
     2641 ggtcctcctt gtgtctgcgc cgcagtatgc gccgccacgc cgaataaagc aagtagttac
     2701 ttgtgatatt cggtgggcgc gagtcgcagg ccaggatggc gtcgtgacgg ggttcgacgt
     2761 ggccgtggtc ggcggaggac tggtgggtgc ggcgaccgcg tacgagctcg cgattggcgg
     2821 tgcccgcacc gtcgtctacg accgcgccga cgccgggcgt gccaccggcg cgggcgccgg
     2881 gatcttgtcg cccgagacgg cagtgcgcga cgacgcggcg tggaccgagc tgtgccgcgc
     2941 cgcgggcgcg cactacgacg cgctcgtgcc gcaactcggc ggcgacaccg gctgggcgcg
     3001 gtgcggcatc ctcgagctcg cgacccgaga aagcgacgtc ggcgcgttcg agtggactgc
     3061 ggcgcgcgcg tccggggcgc gagagatcga ccccgacgac gcgcgcggga tgtgtcccgt
     3121 gctcggagac gtgcagcgcg cgttgcatca tcccgacgcg gcccgcgtcg acgggcgcat
     3181 gatggcggcc gcgttgcagc acgcggcgat tgcgcgcggc gtcgactggc gcgccggcgc
     3241 agccccaccc gtcaccgaga tcggcgccga ggcagtcgtc atcgcgggcg gcgcgtggac
     3301 acccgagacg agcgcgcagg tcggtgttgc acttccgatc acaccgctgc gcggtcagat
     3361 catgcacctc gacctccccg agcacgacac tgcgtcgtgg ccgatcgtgc agccggtgtt
     3421 cgggcactac atggtgccgt gggccgatcg ccacgtcgcg gtcggtgcga ccgtcgaaga
     3481 tgcgggcttc gcggccgagg tcacggcggg cggcgtgcag gagatcctgc gggaggcgtt
     3541 gcgcgtcatg cccggcctcg cgccggcgcg gttgcgggaa gtgcgggtcg ggctgcggcc
     3601 gtgcagcgcc gacgaccttc ccgtcatcgg cccgctcgtc ggcaacgtgt tcgtgtgcac
     3661 cgggcacggc gcgaacggct tgctgctcgg tccggtgagc gggaggttgg tcgcggattg
     3721 tgtcctaagc cgcgaatccc aggtcgacct cgcgccgttc acgcccgccc gattcgtttg
     3781 aacatacgat gcccgccacg ggaggtgcgg ggatgggtgt gcggggttcc ttcggcatgt
     3841 tgctgtgctg tttcgtcgcg ctcgccgggt gcggcggtga cgacgactcg gccgcgagcg
     3901 acgacgacgt cacgacgaca tcgaacgaag tgacgacgtc gaccatcgcg gcggtaccga
     3961 cactcgacgg tctctacttc ctcaccggca cccagacgtg caagagcgca ctcggccccg
     4021 gcacgacgtc gttcccgttg ttcgccgggt cgacgctcgc gatctccggc gaccgcgcgt
     4081 gggccggcct gcggagtgac ccgccggaag catcggtcac gaccgacggc gcctcgttcc
     4141 acgtcaacgt gacctacagc ggccggacga acgcggcggt gacgacagtt gcgggcgcat
     4201 cgccctcggc cctcgacctc gtgctggacc tgtccggcac gatcagcgac gacgggcaga
     4261 cgatcaccgg gacgggcttc gacaccggcg tgaactgcga gtacgagttc acggggtccc
     4321 gcagcgccat cggcgacacc ggggccggca cggagaagtg cggtggcgaa gccgcgctcg
     4381 tgaaggtcgt ggccgagacg ccgcgcgagg acggtctgac gttcaccgcc gacgacgtca
     4441 tcgtggaagc gatgacgccc gacgggcaga tcgcgcgcta tctgatcggg caggccgcgg
     4501 agggattcgc cgggtgcgag cccgacggct ggaagctcgc cgaccccgac atcgtccggc
     4561 tcgcgctctg cgaatccgaa ctggggaagg agatcgcacc ccaactcgac gtcgactgcg
     4621 gcccgtcgac caccgcgccc tccgcggccc cgacgacgac gccggtcgtg acggcgcccg
     4681 cgaacccggt gtcgggctcg tgtgacgcgc aaggcgcgct cgacgcgacg atcgcgaaac
     4741 atcccgacac gattgcccgc gacttggaca cggtgctcgc gtgcgaagac ggctgggccg
     4801 tcgtcgtctg gtcgaccggt gacgccgatc tcatggaagt gctgcagttg cgcgatggcg
     4861 cctgggtcgc gacgcccggc gccgcgtgta ccggagccgc aggaatcccg gagtcggtgc
     4921 gcttctacgc gtgcatgacc ggctgaacgg acgtcagcgc tcgagcttca gcgcgcggtc
     4981 ggcgatgatg ttcttctgca cctcggtcgg gcccgcgccg atcgtcgtgt agcgctgcca
     5041 gacgtagttg cgggcccaca cgccgccgtc gatcgcgccc ggcgcaccct tcaacaacgt
     5101 gccggtcggg ccgaggatgt cgagcgcgag ctcggccagc cagagcgaga tgtagcccca
     5161 ctgcagcttg gcgagcggta cttcgggcca gcccttctcg cccttcaaga ccttcgacaa
     5221 cgcgcgtgcg atcagcagcc gcgtgtattc gatctgcgtg tgcagccgcg cgacccgttc
     5281 gcggatcgcc gggtcctcga gcgcttgggg gttctcggcc ttcgccgtct gcaacaggcg
     5341 gcgaaggtct tgcgccatcg agatggcctg cccggagctg ccgacgcgct cttggccgag
     5401 cgcacccatc gacaccatcc agccgaggtt ctcgtcgccg agccggtcgc cgaccggcac
     5461 cttcacgtcg gtgaagaaca cctcgcagaa catcgagtcg ccggtgatct cgcggatcgg
     5521 ccggatgtcg ataccgggca gctccatgtc gacgatgaac gcggtgatgc cttcgtgctt
     5581 cttgccacgg gagatcgcgg ttggatcggt gcggaacaag aagagacccc acttcgcgat
     5641 ctgcgcggag gagatccaga tcttctgccc gttgacgacg tagtgcgtgc cgtcgtcgga
     5701 gaggatcgcg gtcgtgcgca ggttcgcgag gtcggaaccg gcctgcggct cactgaagcc
     5761 ctggcaccag tgctccgacg cgtcgaggat gcccggcagc cacttcgact tctgctcctc
     5821 ggtgccccac gcgatgatcg acgggccgat ctgcacgatg ccgttcgcgt tgtagatccc
     5881 gggcgtgcgg tacttcgaca tcacttgcgt gtagatcact tgctgcgacg tcgtcgcggc
     5941 gcgcccgccc cactcctcgg gccacaagat cgcggaccag cgcccttcgt tcagcttgtg
     6001 ctgccacgcc ttgcgcttgt ccatgacgcc catgacgccg cgcgagggct ccaggtcgtc
     6061 gtcggtccac tcgtcgagga agggcgggag ctccttgtcg aggaacgctt cgagctcctc
     6121 tttgaacgcg aggtcctcgg cttcccaggc gaaatccacg accctgacac tagtgtcagg
     6181 tttcgtggac ttcactttct cggcggatca ggacgcgctg cgggacacgg tgcgctcgtt
     6241 catgcgggcg gaatcgccca aggaatacgt gcgccgcatg tccgagcacg acgacatcgg
     6301 cgtcgagccg gcggtgtggc ggaagctcgt cgacctgggc tggacggcgc tgctcgtgcc
     6361 cgaggaacag ggcggtctcg ggctcggcgt cgtcgacgcg gtcgtggtgc aagaggagat
     6421 gggacgcgcg acgttccccg gtccgtactt ctcctccgcg atcctcgcga cgctcgccgc
     6481 gcgcgcactc ggcctcgacg aacagctcgc ggcgctcgcg gccggcgcgc agcggggcgc
     6541 ggtcgcgctc gaagagcaag gctggggtga tcccgtcgaa cgcgcacgcg tacgcgccaa
     6601 cggtcgcggc gcgcgcttca agctcgacgg tacgaagccg ttcgtgatgg acggccacag
     6661 cgccgactgg gtactcgtca ccgcccgcac tcgtgaaggt ctacagacgt tcctggtcga
     6721 gaagccggcc gcgccggtcg tgtcgagcct cgacatcacg cgcaagttcg cccgtctcga
     6781 gttcgaggac acgcccgcga cgttggtcgg tccgcccggc gatcacaccg cgatctggca
     6841 tcgcgtcgcc gacgacgcgg ccgtccttct cgcggcggaa ctgatcggtg tgtgcgaagc
     6901 agcgaccgag ctcgcgctcg actacgcgca ggcgcgcgaa gtgttcggca agccactgtc
     6961 gaagttccag gtgacgcggc acaaggccgt cgacatgctg caccagatcg agctggcgaa
     7021 ggtcggcgtg cactacgcgg cgtgggcctc cgacgtcgac gcgcccgatc gcgagtcggc
     7081 ggccgcgatg gcgaagtcgt acgcggccga agcggcgaac tacatcggcg ccgagtgcat
     7141 ccagatccac ggcggcatcg gcttcacgtg ggagaacgac gcgcacatct acttgcggcg
     7201 agccaaggtc gacgacctgt tgctgggcga ccagaactgg cagcgcggcc gggtcgccga
     7261 cctgtacttc gccggcctct agggcttccc ggccgggggt cgaggtgaca gatggacgct
     7321 gtggttgatg gtcatgctgc tttgcggttg cggtggctgt cgaggacggt tcgtggtgtg
     7381 cggccgcgca tgaagtcgct gtgg
//
LOCUS       DASUGA010000377         2315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_896567, whole genome shotgun sequence.
ACCESSION   DASUGA010000377 DASUGA010000000
VERSION     DASUGA010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896567"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..844)
                     /locus_tag="VFX21_07445"
     CDS             complement(23..844)
                     /locus_tag="VFX21_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009472439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5095828.1"
                     /translation="MQDKVAIVTGSTRGIGRATALRFAREGARVVVTGRTEDAGRAVE
                     DEIRNAGGEGVFVPTDLAREEDVVRMVTTAMERYGALTTLVNNAAPTELMGPGRLDRR
                     VTELENDAWDSIMLVALKAVIWSCKHAIPAMTASAGPDGGGGAIVNISSAASILGTPG
                     LDTYTAAKGALNALTRSLAVEYAPDNIRVNCIVSGMVLTSEGAWKMMEDPVIGGATKA
                     MHLTRLGMPDDIANAALFLASDEAAFVTGAIIPVDGGVTARMPVPDISAADLSLD"
     gene            931..1635
                     /locus_tag="VFX21_07450"
     CDS             931..1635
                     /locus_tag="VFX21_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095829.1"
                     /translation="MASVSDLLRTPHWSTDIPLETPHLRLDVLAERGFVILRDQDPPV
                     PDDEFLGLEYMDWKSGGDTNFAPIATADGQLDCRGFWKPGDEKPDKGGRWTSNAPRCP
                     TLVANVEEVGADFGRVRVIKLEPQGYEDALRQIHRDDNNRFNPDGDGWVVRQWCELTD
                     NPGAFMVLMEQGDDGLPDPATEVHVELHRGSRFVVDTQRLWHVVCNPSGTTRYALIAS
                     FESGPPLDRWIRANLP"
     gene            complement(1678..2115)
                     /locus_tag="VFX21_07455"
     CDS             complement(1678..2115)
                     /locus_tag="VFX21_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018385818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5095830.1"
                     /translation="MTEEQDVDAVRAANAAFYSAFEAGDLDAMADVWEHSDRIAVTHP
                     GWPTLRGWPSVAGSWDAIFRNTPYIQFVLTGDEVAVVGDVGWVTLDENILQATGSPDG
                     GAADTQELSGARIAAVNVFVRAGDGWRMVLHHGSPVSAGAPDG"
BASE COUNT          354 a          843 c          758 g          360 t
ORIGIN      
        1 ggctcctttg cgctcagcgg attcagtcga ggctgaggtc ggcggccgag atgtcgggca
       61 ccggcatgcg cgccgtgacc ccgccgtcga ctgggatgat cgcgccggtg acgaacgccg
      121 cctcgtcgga cgcgaggaac agcgccgcgt tcgcgatgtc gtcgggcatc ccgagccgcg
      181 tgaggtgcat tgccttcgtc gcgccaccga tgacgggatc ctccatcatc ttccacgcgc
      241 cttcgctcgt gagcaccatg cccgacacga tgcagttcac gcgaatgttg tcgggcgcat
      301 actcgacggc gagcgaccgc gtgagtgcgt tgagcgcccc tttggccgcg gtgtaggtgt
      361 cgagcccggg cgtcccgagg atcgacgccg ccgacgagat gttcacgatc gcgccaccgc
      421 ctccatcggg ccccgccgac gcggtcatcg ccgggatcgc gtgcttgcac gaccagatca
      481 ccgccttgag cgccacgagc atgatcgagt cccacgcatc gttctcgagc tcggtgacgc
      541 ggcgatcgag tcgcccgggg cccatgagct ccgtgggggc ggcgttgttc acgagcgtgg
      601 tgagcgcgcc gtaccgctcc atcgcggtgg tcaccatccg caccacgtcc tcttcgcgcg
      661 cgagatccgt cgggacgaac acgccttcgc cgcccgcgtt gcggatctcg tcctccaccg
      721 cgcgtcccgc gtcctcggtg cgaccggtca ccacgacgcg cgcgccctca cgtgcgaacc
      781 gcagcgcggt cgcgcggccg atcccgcgtg tcgaccccgt gacgatcgcc accttgtctt
      841 gcattcgagt agccgccgcc attgcgggag catggcacag gcgccaagag atcgcgttgg
      901 ccgcgatcgt ctctctacac tttcgggtac atggcctccg tgtccgatct cctgcgcacg
      961 ccgcactggt ccaccgacat cccgctcgag acgccgcacc tccgcctcga cgtcctggcc
     1021 gagcgcgggt tcgtgatcct gcgcgaccag gatcctccgg tccccgacga cgagttcctc
     1081 ggactcgagt acatggactg gaagagcggc ggcgacacca acttcgcgcc gatcgccacc
     1141 gccgacgggc agctcgactg ccgtggcttc tggaagcccg gcgacgaaaa gcccgacaag
     1201 ggtggccggt ggacgtcgaa cgcgccgcgg tgcccgacgc tcgtcgcgaa cgtcgaggaa
     1261 gtcggcgccg acttcggacg ggtgcgcgtg atcaagctcg agccgcaggg ctacgaggac
     1321 gcgctgcgcc agatccaccg cgacgacaac aaccgcttca accccgacgg cgacgggtgg
     1381 gtcgtgcggc agtggtgtga gctcaccgac aatccgggtg cgttcatggt cctgatggaa
     1441 cagggcgacg acggactgcc cgaccccgcc accgaagtgc acgtcgagct gcaccgcggc
     1501 tcacgcttcg tcgtcgacac gcaacggctg tggcacgtcg tgtgcaatcc cagcggcacg
     1561 acgcgctacg cgctcatcgc cagcttcgag agcggtcccc cgctcgaccg ctggatccgc
     1621 gcgaacttgc cctgacggga ttcaggcctg ccgtagcccg cgcgtcggcg cgcgccgtca
     1681 gccgtcgggc gcgcccgcgc tcacaggcga accgtggtgc agcaccatgc gccagccgtc
     1741 gcccgcacgc acgaacacgt tgaccgcggc gatgcgcgcg cccgagagct cctgtgtgtc
     1801 ggcggcaccg ccatccgggc tccccgttgc ctggaggatg ttctcgtcga gcgtcaccca
     1861 cccgacgtcg ccgacgaccg cgacttcgtc gccggtgagc acgaactgga tgtacggcgt
     1921 gttgcggaag atggcgtccc acgagcccgc cacactcggc caaccacgca acgtgggcca
     1981 acccggatgc gtcacggcga tgcgatccga gtgctcccac acgtcggcca tggcgtcgag
     2041 gtcgccggct tcgaacgccg aatagaacgc tgcgttcgcg gcgcgcacgg catcaacgtc
     2101 ctgttcctcc gtcatcgcgc gcaatcgtcg cacggcgcga ccgctggcgt caggctgctt
     2161 ctcggatcac ctggtcacgc aaggagcacc agtggtgccc ggtgacgacg atccagtcga
     2221 ggagcacgac tccttcgtcg gcgcagctgc gccgcaaggt gacgaactgc tccacttcat
     2281 cgcgcgtggg gagccgcggg gtgcccggtt cgacc
//
LOCUS       DASUGA010000378         6071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1018854, whole genome shotgun sequence.
ACCESSION   DASUGA010000378 DASUGA010000000
VERSION     DASUGA010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018854"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VFX21_07460"
     CDS             complement(<1..198)
                     /locus_tag="VFX21_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095831.1"
                     /translation="MCTVIEHLFTIVGPPRRMYFAAVAFETMPAAVYTGKGAVELQDV
                     PVPQPGDGDVLIEVSHCGICGT"
     gene            185..709
                     /locus_tag="VFX21_07465"
     CDS             185..709
                     /locus_tag="VFX21_07465"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX5095832.1"
                     /translation="MTVHIALLRGINVGGKGVLKMSDLRDICAGAGCTDVATYIQSGN
                     VVFSHSTASADSVASMLERALQQATGRDVAVMVRSRAQLQKVVDKNPYKNAEPQTLHV
                     AFLAAAPAAAAINAFDRAKYAPEEFVVAGRDVYLHLPNGVGKTKLLPPLTAKLPAATV
                     RNWNTVQKLLEMAG"
     gene            complement(744..1595)
                     /locus_tag="VFX21_07470"
     CDS             complement(744..1595)
                     /locus_tag="VFX21_07470"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX5095833.1"
                     /translation="MVNTWDPGYGPLVETRARIATWNIWARYGPWEERAPAILETLRA
                     VDADIVGLQEVWDDGTRNQARELADALGYESCVWERNLTFPDGVHAGNAIISRWPIVR
                     HDVRLLPRESGEARDDEGEERLCVFAEIDGPRGLIQVFCAHLSWRDDHSAVRQEQVRA
                     ISEFVRDMRPRTFPAVVVGDLNAEPESDEMRALVGLRAVPVRGIRFRDAWRSVHPGDP
                     GYTVDNRNPFDAANLFLDARIDYVMVGAPKLGGVGHVCDARLVGNEPVAGQWASDHFG
                     VVADLRY"
     gene            complement(1615..2526)
                     /locus_tag="VFX21_07475"
     CDS             complement(1615..2526)
                     /locus_tag="VFX21_07475"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX5095834.1"
                     /translation="MTATDLPLIPVPAAPAGHEYRHITVERHAGPCGAVIGGVDVATE
                     LADDVVAEIRRALLDHGVVFFREQSLTPEEQVRFSRRFGPFSPVPFVQAIAEHPEVIA
                     VVRDPSEPIGLTFGSLWHSDFSFLAEPPMGSILHAIEVPPYGSDTLWANQYIAYKTLS
                     PGMQRMLEGLVGVHSAVNAYSPKMQVIHDSFRGMSVQTSDDANRIQEHPVVRVHAETH
                     KRALFVNAQYTVGLAGFHPHESKPLLDQLFAHSTQDSFTCRWKWAAGDVAFWDNRCVQ
                     HMVMADVAGHRRYMHRTTVAGEAPARV"
     gene            2603..3172
                     /locus_tag="VFX21_07480"
     CDS             2603..3172
                     /locus_tag="VFX21_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HEX5095835.1"
                     /translation="MTENTVMLQNVIGEMSRLVAGLDQSQFQRPSPCEGWSVADVLNH
                     VTAGAIMFAIAAEDGAVSDEKMGELLGNDNLGANPQASFGQAADRAIGAFSVPGVMDK
                     VVKLPFGEMPASVALDIAVFDVATHACDSAYGTGQTIRDTATFEEALEYGRKMIGPEL
                     RGTGLFEAEQPPRSDAIQDRLLAFGGRKI"
     gene            complement(3250..3453)
                     /locus_tag="VFX21_07485"
     CDS             complement(3250..3453)
                     /locus_tag="VFX21_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX5095836.1"
                     /translation="MAQGTVKFFNAEKGYGFISREGGEKDIFVHYSNIQGNGYKTLEE
                     GQRVEFDVAPGRKGDEAQNVRVV"
     gene            3563..4063
                     /locus_tag="VFX21_07490"
     CDS             3563..4063
                     /locus_tag="VFX21_07490"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX5095837.1"
                     /translation="MRDLRHVVLVGLMGSGKTTVGRLVARRLGWPLVDNDVELGRRIG
                     MTAREFTAREGLDALHRAEAETLAAMLDVSEPSVIGAAASVVESPLMRDRLERHYVVW
                     LDADPEVLAARAANGSHRPHLPAAQLQAARSALYEQISALRVDTTHMSPEDAAAYVAE
                     ALADGR"
     gene            4096..5928
                     /gene="typA"
                     /locus_tag="VFX21_07495"
     CDS             4096..5928
                     /gene="typA"
                     /locus_tag="VFX21_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019872785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX5095838.1"
                     /translation="MPAREDLRNVAIVAHVDHGKTTLVDALLWQSGAFRANQDVAERV
                     LDSNDLEREKGITILAKNTAIKYGESTFNIIDTPGHADFGGEVERGLAMVDGVLLLVD
                     ASEGPLPQTRFVLRKALESHLPVILVVNKVDRPDARISEVVDEVYELFIDLGAEEHHI
                     EFPIVYANARAGWASTDPDVEGSDLKPLCELILEHIPAPMYEEDHPFQAMVTNLDASP
                     YVGRLALCRVRNGRITRGQQVAWCRIDGSIEKAKITELYMTEALDRVDVNEAGPGDII
                     AIAGFPEITIGETLADADDPRPLPPLTVDEPSLSMTVGINTSPLAGKEGQKLTARLVE
                     ARLTQELVGNVSLRVLPTERPDAWEVQGRGELQLAVLVETMRREGYELTVGKPQVVTR
                     EIDGKLHEPVERLTIDVPEEYVGVVSQLIALRKGRMEQMVNHGTGWVRLDFRVPSRGL
                     IGFRTEFMTETRGTGMLHHVFDRYEPWHGALRTRPTGSLVADRRGYSNTFALMNLQER
                     GQMLVGPAVEVYEGMIVGENARSDDMDVNITKEKKLTNMRASGSDNTERLVPPRPMSL
                     EQALEFIADDECVEVTPHNVRLRKVELDASIRGRTTKNATRGKS"
BASE COUNT         1007 a         2092 c         2043 g          929 t
ORIGIN      
        1 ggtgccgcag atgccgcagt gcgagacttc gatcaggaca tcgccgtcgc cgggttgggg
       61 tacgggcacg tcctgtagtt cgaccgcgcc tttgccggtg tacacggccg ccggcatggt
      121 ctcgaacgcc acggcggcaa aatacatgcg gcgcggcggc ccgacaatcg tgaagaggtg
      181 ttcgatgacc gtgcacatcg cgctcttgcg cgggatcaac gtcggcggca agggcgtgct
      241 caagatgagc gacctgcgcg acatctgcgc aggcgcagga tgtaccgacg tcgcgacgta
      301 catccagagc ggcaacgtcg tcttctcgca ctcgaccgca tccgccgatt cggtggcgag
      361 catgctcgaa cgcgcgctgc agcaggcaac cggccgtgac gtcgcggtga tggtgcggtc
      421 gcgcgcgcag ctccagaagg tcgtcgacaa gaacccgtac aagaatgcgg agccgcagac
      481 gttgcacgtc gcgttcctcg cggccgcgcc cgcggccgcg gcgatcaatg cgttcgatcg
      541 ggcaaagtac gcgcccgagg agttcgtcgt cgcgggccgc gacgtctatc tgcacctgcc
      601 aaacggcgtc gggaagacga agctgttgcc gccactcacc gcgaagttgc cggctgcgac
      661 agtgcgcaac tggaacaccg tgcagaagct gctcgagatg gccggatgac cgagcacgcg
      721 gtcgcgatcg tcggaaccgc ggcctagtag cgcaggtcgg cgacgacgcc gaagtggtcc
      781 gaggcccact ggcccgcgac cggctcgttg ccgacgaggc gggcgtcgca gacgtggccg
      841 acgccaccca gcttcggtgc gccgaccatg acgtagtcga tgcgcgcgtc gaggaagagg
      901 ttggcggcgt cgaacgggtt gcggttgtcg acggtgtagc cgggatcgcc gggatgcacc
      961 gaccgccacg cgtcgcggaa gcggatgccg cgcaccggca ccgcccgcag gccgacgagc
     1021 gcgcgcatct cgtcgctctc gggctcggca ttgaggtcgc cgaccaccac ggccggaaat
     1081 gtgcgcggcc gcatgtcgcg tacgaactcg ctgatcgccc gtacttgctc ctgccgcacc
     1141 gcgctgtggt cgtcgcgcca gctcaggtgc gcgcagaaca cctggatcag gcctcggggg
     1201 ccgtcgatct ccgcgaacac gcagaggcgc tcttcgccct cgtcgtcacg cgcctcgccg
     1261 gactcccgcg gcagcaggcg gacgtcatgg cgcacgatcg gccaccgcga gatgatcgcg
     1321 ttgccggcgt gcacgccgtc ggggaacgtc agattccgtt cccagacgca cgactcgtag
     1381 ccgagagcgt ccgcgagctc gcgcgcctga ttgcgcgtgc cgtcgtccca gacctcctgc
     1441 aacccgacga tgtcggcgtc gaccgcgcgc agcgtctcga ggatcgcggg cgcccgttcc
     1501 tcccacggcc cgtagcgcgc ccagatgttc cacgtcgcga tgcgcgcccg cgtctcgacc
     1561 agcggcccgt accccggatc ccaggtgttc accatgtcgc tgccggtgcc gtcgctacac
     1621 ccgcgccgga gcctcgcccg cgacggtcgt gcggtgcatg taccggcggt ggccggccac
     1681 gtcggccatg accatgtgct gcacgcatcg gttgtcccag aacgcgacgt cgccggccgc
     1741 ccacttccac cggcacgtga acgagtcctg ggtcgagtgt gcgaacagtt gatcgagcaa
     1801 cggcttcgac tcgtgcggat gaaagcccgc gagccccacc gtgtactgcg cgttgacgaa
     1861 caacgcgcgc ttgtgcgtct ccgcgtgcac ccgcacgacc ggatgctcct ggatgcggtt
     1921 cgcgtcgtca ctcgtctgca cgctcatccc gcggaagctg tcgtggatca cctgcatctt
     1981 cggcgagtac gcgttcaccg cgctgtgcac gccgacgagc ccctcgagca tgcgctgcat
     2041 gcccggcgac agcgtcttgt aggcgatgta ctggttcgcc cacaacgtgt cggagccgta
     2101 cggcggcacc tcgatcgcgt gcaggatcga ccccatcggc ggctcggcca ggaacgagaa
     2161 gtcggagtgc cacagcgatc cgaacgtcaa cccgatgggc tcgctcgggt cgcgcacgac
     2221 ggcgatcact tcgggatgct cggcgatggc ctgcacgaac ggcaccgggc tgaacggtcc
     2281 gaaccggcgg ctgaagcgca cttgctcctc cggcgtgagc gactgctcgc ggaagaacac
     2341 gacaccgtgg tcgagcagcg cccggcggat ctcggcgacc acgtcgtcgg cgagctcggt
     2401 cgcgacgtcg acaccgccga tcacggcgcc gcacgggccc gcgtggcgct cgacggtgat
     2461 gtgccggtac tcgtggccgg ccggggccgc gggtacgggg atcagcggga ggtcggtcgc
     2521 cgtcatgggg caaacgctac gccgttgatc cgcgggcgtg ctcggggcga acattcatcg
     2581 gatccgaacc cgggagggat cagtgacaga gaacaccgtg atgctgcaga acgtcatcgg
     2641 cgagatgagc cgcctcgttg ccggcctcga ccagtcgcag ttccaacgcc cgtcgccgtg
     2701 tgaggggtgg agcgtggccg acgtcctcaa ccacgtcacg gcgggggcga tcatgttcgc
     2761 catcgcggcc gaggacggcg cggtctcgga cgagaagatg ggcgagctgc tcggcaacga
     2821 caacctcggc gccaacccgc aggcgtcgtt cggccaggcc gcggaccggg cgatcggcgc
     2881 gttctcggtc ccgggcgtca tggacaaggt ggtgaagctg ccgttcggcg agatgccggc
     2941 gagcgtcgcg ctcgacatcg cggtgttcga cgtcgcgacg cacgcgtgcg acagtgcgta
     3001 cgggaccggc cagacgatcc gcgacacggc gaccttcgaa gaggcactcg agtacggccg
     3061 caagatgatc ggtcccgaac tgcgcgggac cggcctgttc gaagccgaac agccaccgcg
     3121 cagcgacgcc atccaagacc gcctcctcgc cttcggcggc cgcaagatct gacaaacaaa
     3181 caagccgccg gatttctccg gcggcctgct gattcttcga gaccgagatc tcgcgtgatt
     3241 ggtcgaagct cagaccacgc gaacgttctg agcctcgtca cccttgcggc ccggcgccac
     3301 gtcgaactcg acgcgttgac cctcttcgag ggtcttgtag ccgttcccct ggatgttcga
     3361 gtagtggacg aagatgtcct tttctccgcc ttcacgcgag atgaacccgt agcccttctc
     3421 ggcgttgaag aacttgacag tgccttgtgc caacgcagtt gcttctttct tggtgtgagc
     3481 ggaaccaacc cccgctcgtt gtcgtcgacc aatgtagcgg gcgtttcgca gaaccggaca
     3541 cgaggcgggg tagaggtcca cggtgcgtga tctccggcac gtcgtgctcg tcgggttgat
     3601 gggctcgggc aagacgaccg tgggccgcct ggtcgcacgg cggctcggct ggccgctggt
     3661 cgacaacgac gtcgaactgg gccgccggat cggcatgaca gcccgcgagt tcacggcccg
     3721 tgagggcctc gatgccctgc accgggccga ggccgagacc ctcgcggcca tgctcgacgt
     3781 ttccgagccg tcggtgatcg gggccgcggc gtcggtggtc gagtcaccgc tcatgcggga
     3841 tcggctcgag cggcactacg tcgtgtggct cgacgccgac cccgaggtgc tggcggcccg
     3901 agcggcgaac gggtcgcacc gcccccacct gccggccgcg cagctccagg ccgcccgcag
     3961 tgcgttgtac gagcagatct ccgcgctccg tgtcgacacg acgcacatgt cgccggagga
     4021 cgcggccgcg tacgtggccg aggccctcgc agacggccgg taacctgttc gttcccccgg
     4081 atctggagtt ctgttatgcc cgcgcgcgaa gacctgcgca atgtggcgat cgtcgcccac
     4141 gtcgaccacg gcaagaccac cctcgtcgac gcgctgctgt ggcagtcggg tgcgttccgc
     4201 gccaaccagg acgtcgccga gcgcgtcctc gactcgaacg acctcgagcg cgagaagggc
     4261 atcaccattc tcgccaagaa caccgccatc aagtacggcg agtcgacgtt caacatcatc
     4321 gacacgcccg gccacgccga cttcggcggt gaggtcgagc ggggtctggc catggtcgac
     4381 ggcgtgttgc tgttggtcga cgcgagcgaa ggtccgctgc cgcaaacccg tttcgtgctc
     4441 cgcaaagcgc tcgaatcgca tctgccggtc atcctcgtcg tcaacaaggt cgaccggccc
     4501 gatgcccgca tctcggaagt cgtcgacgag gtctacgagc tgttcatcga cctcggcgcc
     4561 gaggagcacc acatcgagtt cccgatcgtc tacgcgaacg cgcgtgcggg ctgggcgtcg
     4621 acggaccccg acgtcgaggg cagcgacctc aagccgctgt gcgagttgat actcgagcac
     4681 atccccgcgc ccatgtacga agaagaccac ccgttccagg cgatggtgac caacctcgac
     4741 gcgtcgccgt acgtcggccg cctcgcgctg tgccgcgtgc gcaacggccg catcacgcgc
     4801 gggcaacagg tcgcgtggtg ccgcatcgac ggttcgatcg agaaggcgaa gatcaccgag
     4861 ctgtacatga cggaggcgct cgaccgcgtc gacgtcaacg aagcggggcc gggcgacatc
     4921 atcgcgatcg cgggcttccc cgagatcacg atcggcgaga cgctcgccga cgccgacgac
     4981 ccgcgcccgc tgccgccgct caccgtcgac gagccgagcc tgtccatgac agtcggcatc
     5041 aacacgtcgc cgctcgccgg caaggaaggt cagaagctca ccgcccggct ggtcgaagct
     5101 cgtttgactc aagagctcgt cggcaacgtc tcgttgcgcg tgctcccgac cgaacggccc
     5161 gacgcgtggg aagtgcaggg ccggggcgag ctgcagctcg cggtgctggt cgagaccatg
     5221 cggcgcgagg gctacgagct gaccgtcggc aagccgcagg tcgtgacgcg cgagatcgac
     5281 ggcaagctgc acgaaccggt cgagcggctg acgatcgacg tgcccgagga gtacgtcggc
     5341 gtcgtgtcgc aactcatcgc gctgcgaaag ggccgcatgg agcagatggt gaaccacggc
     5401 acgggctggg tgcgcctcga cttccgtgtg ccgtcacgcg ggctcatcgg ctttcgcacc
     5461 gagttcatga ccgagacgcg cggcaccggc atgctccacc acgtgttcga ccgctacgag
     5521 ccgtggcacg gcgcgctccg cacgcgtccc accggcagcc tcgtcgcgga tcgccgcggc
     5581 tacagcaaca cgttcgcgct catgaacctc caggagcgcg ggcagatgct cgtgggccct
     5641 gcagtggagg tctacgaggg gatgatcgtc ggcgagaacg cacgctccga cgacatggac
     5701 gtcaacatca ccaaggagaa gaagctcacc aacatgcggg cgtcgggctc cgacaacacc
     5761 gagcgcctgg tgcccccgcg gcccatgtcg ctcgagcagg cgctcgagtt catcgccgac
     5821 gacgagtgcg tcgaggtcac cccgcacaac gtgcggctgc gcaaggtcga gctcgacgcc
     5881 agcatccgcg gccgcaccac gaagaacgcg acccgcggca agagctgatc gtcctgcctc
     5941 gcacgatcgc acgccgcttc ttccgggcga actgaccgcc tctacgatct gggcgccatg
     6001 cgagtgcctc ggttgagccc cgccgcgtac cggcagatca ccctggtcgc cgcgatcctg
     6061 ctcgcggtca t
//
LOCUS       DASUGA010000379         3541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_572347, whole genome shotgun sequence.
ACCESSION   DASUGA010000379 DASUGA010000000
VERSION     DASUGA010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572347"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1345
                     /locus_tag="VFX21_07500"
     CDS             110..1345
                     /locus_tag="VFX21_07500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5095839.1"
                     /translation="MTDTVDPTRAEGAPTGGSLLTPRFVLVVTAGLCYFVSLAMLTPV
                     IPHYVEDELNGGSLAVGVAVGSFAFGAVVLRTYAGRLGDQIGRRVLIVSGAGIVAVTT
                     AAYGLVPSLWWIVLMRLVTGFGEAAFFVGAATMITDLAPPHRRGEAVSYWSIAVYGGL
                     SFGPALGEAVRGDDRYTAAWLVSAGFAAAAMLVGLMTREVARSAPPAEKGHLIHRAAV
                     LPGTVLFLGLIPLAGFSAFMPLYAEDTLNIKAGPIFLLYGMLVLIVRIVGARLPDRLG
                     ARVSGTGALAFSGLGIAIMAIWPTVGGLVAGTIVFAAGMSLMYPALLLMSLTGVPDNE
                     RASVVGTFSSFFDLSQGIGAPICGIAVVLAGDRGAFAAGAITAAFGLFVLWKFGPSAE
                     RAALARGGDAAVVPPENFA"
     gene            complement(1277..2509)
                     /locus_tag="VFX21_07505"
     CDS             complement(1277..2509)
                     /locus_tag="VFX21_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013426621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX5095840.1"
                     /translation="MTNSGPLSGLRVVEVSMLGPGDLTRNLADLGADIIKVEPPQGDY
                     GRRMTWPIVEGVSLLFLHVSRGKRSIVLDLRTEEGVDIFKELVRDADAVVEAMRPGGL
                     ARRGLGYEELKKVNPKIVFMTISGYGMTGPYRDMPSHGIAYDVWAGIVQPKIDDEGFA
                     YMPEHVSIGINAGPVYGALGLLAGVMQARATGEGCQMEIAQSDAAAAIDWLRSETWKA
                     YERPESEVTGNEADNFERRAPGTAGMESGVRYQFYATKDEKFVLFMASEREFWKNFCE
                     GVGRAELFEKWPGSQYGDHARGNRELQVILRDIFATKTSDEWIEFGRVQNTPICPANS
                     PKTLPDDPQFKDRLGWLPASALGAEMLPCPIKIVDGELPVPAKAPTVGQHTDDVLREV
                     LGWDDGRIAAARESGALG"
     gene            2548..>3541
                     /locus_tag="VFX21_07510"
     CDS             2548..>3541
                     /locus_tag="VFX21_07510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5095841.1"
                     /translation="MPSLLVTNDFPPKMGGIQSYLYELWRRLPSAETTVLTTPYDGAA
                     AFDARQGFRVERTKEKVLFPTKSLARRIDALAREVRADAIFLDPMLPLAQLGPSLTAA
                     PYVVIAHGAEITLPGRLPLSNRLARRVLRGAAGVVAAGTYPAREAVRAAAQALPGLII
                     PPGVDPQRFTPVDDETRRATRKRFGLDPEQPLVLGLSRLVPRKGFDIVIDAVRELDGV
                     QLAIAGSGRDRKRLERRAQGRAHFLGRVADDDLPALYACADVFAMCCHDRWGGLEAEG
                     FGIVFLEAAACGVPSVAGRSGGSHEAVVDGETGYVVEPRSVPAVRDAIRQLCADA"
BASE COUNT          463 a         1219 c         1231 g          628 t
ORIGIN      
        1 gcgaatgtgc ggcatgggtc acacggaagg gtcgccctct tcgttgcatt cggcacgaca
       61 ggaactcgga cagtgggccg gcgggtcgtc cccgtagcat cccgggacca tgaccgacac
      121 cgtcgacccg actcgcgccg agggcgcgcc gaccggtgga tcgttgctga cgccccgctt
      181 cgtgctcgtg gtgacggcgg ggctctgcta cttcgtctcg ctcgcgatgc tgacgccggt
      241 catcccgcac tacgtcgagg acgagctcaa cggcggcagc ctcgcggtgg gagtggccgt
      301 cgggagtttc gcgttcgggg cggtcgtgct gcgcacgtac gcgggccgcc tcggtgatca
      361 gatcgggcgt cgcgtcctca tcgtcagcgg cgcgggcatc gtcgcggtga ccaccgcggc
      421 gtacgggctc gtgccgtcgc tgtggtggat cgtgctcatg cggctcgtca ccgggttcgg
      481 tgaagccgcg ttcttcgttg gcgcagcgac catgatcacc gaccttgcgc cgccgcatcg
      541 gcgcggtgaa gcggtcagtt actggtcgat cgcggtctac ggcggcctct cgttcggccc
      601 cgcgctcggc gaagcggtcc gcggcgacga tcgttacacc gcggcatggc tcgtcagcgc
      661 gggattcgcg gcggccgcga tgctcgtcgg cctcatgact cgagaagtgg cgcgcagcgc
      721 gccgcccgcg gagaaggggc atctgatcca ccgcgccgcg gtgctgcccg ggaccgttct
      781 cttcctcggg ctgatcccgc tcgccggatt cagcgcgttc atgccgctgt acgcggagga
      841 cacactgaac atcaaggcgg gtccgatctt cttgctctac ggcatgcttg tgctgatcgt
      901 gcgcatcgtc ggcgcccgcc ttccggatcg cctcggcgcg cgcgtctcgg gcaccggtgc
      961 cctcgcgttc agtggtctcg gcatcgcgat catggcgatc tggccgaccg tcggcggcct
     1021 cgtcgccggc acgatcgtct tcgcggcggg gatgtcactc atgtatcccg cgctgttgtt
     1081 gatgtcgttg accggggtgc ccgacaacga gcgcgcatcc gtcgtcggta ccttctcgtc
     1141 gttcttcgac ctgtcgcagg gcatcggcgc gccgatctgc ggcatcgcgg tcgtgctcgc
     1201 aggcgaccgt ggcgcgttcg cggcgggcgc gatcaccgcc gcgttcgggt tgttcgtgct
     1261 gtggaagttc gggccgtcag ccgagcgcgc cgctctcgcg cgcggcggcg atgcggccgt
     1321 cgtcccaccc gagaacttcg cgtagcacgt cgtcggtgtg ttgaccgacg gtcggcgcct
     1381 tcgccggcac cggcagctca ccgtcgacga tcttgatcgg gcacggcagc atctccgcgc
     1441 cgagcgcgga agccggcaac cagcccagcc ggtccttgaa ctgcgggtcg tcgggcagcg
     1501 tcttcggtga gttcgcgggg cagatcggcg tgttctgcac ccggccgaac tcgatccact
     1561 cgtcgctggt cttcgtcgcg aagatgtcgc gcaggatgac ttgcagctcg cggttgccgc
     1621 gcgcgtggtc gccgtactgc gagccgggcc acttctcgaa cagttcggcg cggccgacgc
     1681 cttcgcagaa gttcttccag aactcgcgct cggacgccat gaacaagacg aacttctcgt
     1741 ccttcgtcgc gtagaactgg tagcgcacgc ccgactccat gccggccgta cccggcgcgc
     1801 gccgctcgaa gttgtcggct tcgttccccg tgacctcgga ctccggccgt tcgtacgcct
     1861 tccacgtctc gctgcgcagc cagtcgatcg cggcggcggc atcggactgc gcgatctcca
     1921 tctggcagcc ctcgcccgtt gcgcgcgcct gcatgacacc cgcgagcaac ccgagtgcgc
     1981 catacaccgg gcccgcgttg atgccgatcg acacgtgctc gggcatgtac gcgaagccct
     2041 cgtcgtcgat cttcggctgc acgatgccgg cccagacgtc gtacgcgatg ccgtggctgg
     2101 gcatgtcgcg gtaggggccc gtcatcccgt agcccgagat cgtcatgaac acgatcttcg
     2161 ggttgacctt cttcagctcc tcgtagccga gcccgcgacg ggcgagcccg cccggtcgca
     2221 tcgcctcgac gaccgcgtcg gcgtcgcgca cgagctcttt gaagatgtcg acgccctcct
     2281 ccgtccgcag gtcgagcacg atgctgcgct tgccccggct cacgtgcagg aacagcagcg
     2341 acacgccttc gacgatcggc cacgtcatgc gccgcccgta gtcgccttgc ggcggctcga
     2401 ccttgatgat gtcggcgccg aggtccgcga ggttcctcgt gaggtcgccg ggcccgagca
     2461 tcgagacctc gacgacgcgc aaacccgaga gcggtccgga gttggtcatg gaggtggagg
     2521 ttagatgggt cggtatcgtt cgctcgcatg ccgtcactgc tcgtcacgaa cgacttcccg
     2581 ccgaagatgg gcggcatcca gtcgtatctg tacgagctgt ggcggcggct gccgtcggcg
     2641 gagacgacgg tgctcaccac gccgtacgac ggtgctgccg cgttcgacgc gcggcaaggg
     2701 ttccgcgtcg aacgtacgaa ggagaaggtg ctcttcccga cgaagtcgtt ggcgcggcgc
     2761 atcgacgcac tggcgcgaga agtgcgcgcg gacgcgatct tcctcgatcc gatgctgccg
     2821 ctcgcgcaac tcggcccgag cctgactgcc gcgccgtatg tcgtgatcgc ccacggcgcc
     2881 gagatcacgt tgccgggccg gctcccgttg tcgaacaggt tggcgcgccg cgtgttgcgc
     2941 ggcgcggcag gcgtcgttgc cgcggggacc tatcccgcgc gcgaagccgt gcgggccgcc
     3001 gcgcaggcgc tcccgggcct gatcattcct ccgggcgtcg acccgcaacg gttcacgccc
     3061 gtggacgacg agacccggcg cgcgacgcgc aaacggttcg ggctcgatcc cgagcaacca
     3121 ctcgtgctcg gactgtcgcg gttggtgccg cgcaaagggt tcgacatcgt catcgacgcc
     3181 gtgcgcgagc tcgacggcgt gcaactcgcg atcgcgggta gcggccgcga ccgcaaacgg
     3241 ctggaacggc gcgcgcaggg acgcgcgcac tttctcggcc gtgtcgccga cgacgatctt
     3301 cccgcgttgt acgcgtgtgc cgacgtgttc gcgatgtgct gccacgaccg ttggggtggg
     3361 ctcgaggcgg aagggttcgg catcgtgttc ctcgaagccg ccgcgtgcgg tgtgccatcg
     3421 gtcgcgggcc ggagcggggg atcgcacgaa gcggtcgtcg acggcgagac cggttacgtc
     3481 gtcgagccgc gctcggtccc ggcggtgcgc gacgcgatcc ggcagctgtg cgccgacgcg
     3541 g
//
LOCUS       DASUGA010000380         3261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_846708, whole genome shotgun sequence.
ACCESSION   DASUGA010000380 DASUGA010000000
VERSION     DASUGA010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846708"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VFX21_07515"
     CDS             <1..876
                     /locus_tag="VFX21_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015662882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5095842.1"
                     /translation="RLADAEHAVANRPRGATTPADDVARTLTKAICDAGRGRTAHALA
                     AVTDLQAPRSGSNILRATTSLAVDALQQRSIRRRAPLTNSFLADLCAALGAVDLLVEH
                     PMTTAYPVERSLARARRLFTTGRDDDAILDALVPLTDVACHPRSQVELYTLCALAADR
                     TGRDQSALGSLRRALARAFADDMRAPFMGYGRAAAALLERYAWQLGEHHSYAVAVLED
                     VRQSDPPVFVETLTDRELAVLEYLPTMMSNVEIARQLYVSVNTVKTHLKSVYRKLGVE
                     RRRDAVVRARQLELL"
     gene            complement(889..1275)
                     /locus_tag="VFX21_07520"
     CDS             complement(889..1275)
                     /locus_tag="VFX21_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095843.1"
                     /translation="MDPMDNTRSGIVTFAGVLALIIGFFNVIAGIAAIAEDDQTERAA
                     EVLFGIDITVWGWVWLGIGALQLLTGYLILVRHPIGLMFGVAWAVVSATLTIFTIFSV
                     PLYALMVLALEVLIIYALLENADEFA"
     gene            1418..2140
                     /locus_tag="VFX21_07525"
     CDS             1418..2140
                     /locus_tag="VFX21_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family zinc transporter"
                     /protein_id="HEX5095844.1"
                     /translation="MLAAFGWGALGASALLVGAVIAYQFHPNRRIIAFVMALGTGLLI
                     GSVAFELVDDALDAQSVGAVGALMLVGAVVFVAGDWFLDRQGAGDRKDPTGEQASGSP
                     LAIVLGSILDGIPESFVLGLTVLQGGVSVAFLVSVLLSNLPEGMSSSSGLDAAGWPRK
                     RVLLMWLAVIVVSALSAALGWLLLDPASGRTGALVQAFAGGALLAMVADTLLPEAYET
                     EGPFTGLVVVVGFAISVSLSAV"
     gene            complement(2123..>3261)
                     /locus_tag="VFX21_07530"
     CDS             complement(2123..>3261)
                     /locus_tag="VFX21_07530"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX5095845.1"
                     /translation="NFVENVDDLLHGLDDIQWRTFALALSCAVILLGLKRFAPRVPGA
                     LVVVVFGIAMVRLQDLSAKGVAVLGTVDDGLPSLGFPDPKGWWILVPGAAAIALMSGL
                     ESATAAKILRRPQDPEIDANRELNATGAMNVAAGFVGAYPGGGGLSQSVVNSNAGATS
                     QLSSLTTAVCAVIVLTVATGLLEDLPQAALGVIVIVAVSSFLDPRPLIAMSRVRTRDF
                     GLAIVALVCACLFGMLEGVLVSLIVSVLVLMYQVNKVTIEDVTPPDLDGLMALRPRGL
                     LYFANVQAVNDRTLRMVLDAQPRPRIVIIDFISVQDLEITALDSSRKLVDELLDAGIE
                     PWAAGFSPGEAGMVGRYGPRQELRIFATLDDAIATYRRELDRGQ"
BASE COUNT          503 a         1135 c         1107 g          516 t
ORIGIN      
        1 cgcctcgccg acgccgaaca cgcggtcgcg aaccgcccgc ggggcgcgac gacgcccgcc
       61 gacgacgtcg cgcgcacgct gacgaaggcg atctgtgatg cgggccgcgg gcgtactgcg
      121 cacgcgctcg ctgccgtcac cgatctccag gcaccgcgca gcgggtccaa catcttgcgc
      181 gcgacgacga gcctggccgt cgacgcgctc cagcaacggt cgatccgccg gcgcgctccg
      241 ctcaccaact cgttcctcgc ggacctgtgt gccgcgcttg gcgccgtcga cctgctcgtc
      301 gagcacccca tgacgaccgc ctacccggtc gagcgatcac tcgcccgcgc gcgacggctg
      361 ttcacgaccg ggcgcgacga cgacgcgatc ctcgacgcac tggtgccgct caccgacgtc
      421 gcgtgccatc cacgttcgca ggtggagctc tacacgttgt gcgcgctcgc ggccgaccgc
      481 accggccgtg accagtccgc gctgggttcg ctccggcggg cgctcgcccg cgcgttcgcc
      541 gacgacatgc gtgcgccgtt catgggctac ggccgggccg cggcagcgtt gctcgagcgc
      601 tatgcgtggc agctcggcga gcaccacagc tatgcggttg ccgtcctcga agacgtccgg
      661 cagtcggatc cgccggtctt cgtcgagacg ctgaccgatc gcgagctcgc ggtactcgag
      721 tacctgccga cgatgatgtc gaacgtcgag atcgcgcggc agctctacgt ctccgtgaac
      781 acggtgaaga cacacctgaa gtcggtgtac cgcaaactcg gagtcgagcg gcggcgcgac
      841 gccgtcgttc gcgcgcgcca gctcgagctc ctctgagtgc ttcgtcgatc aggcgaactc
      901 gtctgcgttc tcgagcagcg cgtagatgat cagcacctcg agtgcgagca ccatgagcgc
      961 gtacagcgga acggagaaga tcgtgaagat cgtcaacgtc gcgctcacga cggcccacgc
     1021 cacaccgaac atcaacccga tcgggtgccg tacgaggatg aggtaaccgg tcaacagctg
     1081 caaagcgccg atgccgagcc agacccatcc ccacaccgtg atgtcgatgc cgaagagcac
     1141 ttcggccgcc ctctcggttt ggtcgtcttc ggcgatcgcc gcgatgcccg cgatgacgtt
     1201 gaagaaaccg atgatgagcg cgagcacacc ggcgaaggtg acgatgccgc tgcgggtatt
     1261 gtccatcggg tccacgaccc ctccttttcc aggcgcgatg ctgcctggcg ggcgtcagca
     1321 cttcgtcacc cacgctgggt gaccggcgtc catcgtcgca aatgcccgtc gccggcgggt
     1381 ggatacgggc cgcggagaca cagggagcga ggctgacatg ctggccgcct tcgggtgggg
     1441 cgcgctcggc gcatcggcgc tgctcgtggg cgcggtcatc gcgtaccagt tccatcccaa
     1501 ccgacggatc atcgcgttcg tgatggcgct cggcaccggg ctgctcatcg gctcggtggc
     1561 gttcgagctc gtcgacgacg cgctcgacgc gcaaagtgtg ggcgcggtcg gcgcgttgat
     1621 gctcgtcggc gcggtggtgt tcgtcgcggg cgactggttc ctcgaccggc agggcgcggg
     1681 cgaccgcaag gacccgacgg gggagcaggc gagcggctca ccgctcgcga tcgtgctcgg
     1741 ttcgatcctc gacggcatcc ccgaatcgtt cgtgctcggg ctcaccgtgt tgcaaggcgg
     1801 cgtcagcgtc gcgttcctcg tcagcgtctt gttgtcgaac ctgccggaag gcatgtcgtc
     1861 gtcgagcggc ctcgacgccg cgggctggcc gcgcaagcgc gtcctgctca tgtggctcgc
     1921 ggtcatcgtc gtctccgcgc tctcggccgc actcgggtgg ttgttgctgg accccgcgag
     1981 cgggcgcacc ggcgcactcg tgcaggcgtt cgcgggcggc gcgctcctcg cgatggtcgc
     2041 cgacacgttg ctgcccgagg cctacgagac cgagggtccc ttcaccggcc tcgtcgtcgt
     2101 ggtcggcttt gcgatctccg tgtcattgtc cgcggtctag ctctcggcgg tacgtcgcga
     2161 tcgcgtcgtc gagggtcgcg aagatccgta gctcctggcg cggtccgtag cgcccgacca
     2221 tgccggcctc accgggcgag aagcccgcgg cccacggttc gatcccggcg tcgagcagct
     2281 cgtcgacgag cttccggctc gagtcgagcg cggtgatctc caggtcctgc accgagatga
     2341 agtcgatgat gacgatccgc gggcgcggct gcgcgtcgag gaccatgcgc agcgtccggt
     2401 cgttcacggc ttggacattg gcgaagtaca acaggccccg cggccgcagc gccatgagcc
     2461 cgtccagatc gggtggcgtc acgtcctcga tcgtcacctt gttgacttgg tacatcaaca
     2521 ccagcacgga gacgatgagc gagacgagaa cgccttcgag catcccgaac aggcacgcgc
     2581 acaccagcgc gacgatcgcg agcccgaagt cgcgtgtgcg tacccgcgac atcgcgatca
     2641 acgggcgcgg gtcgaggaac gacgacaccg cgacgatcac gatcacgccc aacgcggcct
     2701 gaggaagatc ctcgagcaac ccggtcgcga ccgtgaggac gatgaccgcg cagactgcgg
     2761 tcgtcagcga cgacagctgg ctcgtcgcgc cggcattcga gttgacgacg ctctgcgaca
     2821 ggccgccgcc gcccgggtac gcgccgacga aaccggccgc gacgttcatc gcgccggtcg
     2881 cgttcagctc gcggttggcg tcgatctcgg ggtcctgcgg ccggcgcagg atcttcgccg
     2941 cggtcgccga ttccaatccc gacatcagcg cgatggcggc cgcgccgggc acaaggatcc
     3001 accatccttt ggggtcgggg aaacccaggc tcggcaggcc gtcgtcgacc gtgccgagca
     3061 ccgcgacgcc tttggcgctc aggtcttgca ggcggaccat cgcgatcccg aacacgacga
     3121 cgacgagcgc gcccggcacg cgcggcgcga agcgcttgag gccgagcagg atcaccgcgc
     3181 agctcagggc cagcgcgaac gtgcgccatt ggatgtcgtc gaggccgtgc aggagatcgt
     3241 cgacgttctc gacgaagttg t
//
LOCUS       DASUGA010000381         3050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1439757, whole genome shotgun sequence.
ACCESSION   DASUGA010000381 DASUGA010000000
VERSION     DASUGA010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439757"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(307..498)
                     /locus_tag="VFX21_07535"
     CDS             complement(307..498)
                     /locus_tag="VFX21_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095846.1"
                     /translation="MSFSVHFDDDTNTLVHDADAYEQEGPLTTFFERNGGSGHLGSAF
                     ACRVASFRTSRIIEIRRLP"
     gene            complement(540..1568)
                     /locus_tag="VFX21_07540"
     CDS             complement(540..1568)
                     /locus_tag="VFX21_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095847.1"
                     /translation="MHEPVVLLDPLFDAAGADAMVELCERFGRYRMYLEHEQLETDIG
                     RGLAQRHDALTNFLRTGGSTGADEPVSALAVRTSYFREEYAYGQTARIDGIEPFLHHP
                     GFVEAAQAIHGRPVIEPAIAYANLMIPGQELAVHTDVPEFRGANRKVIPQWLLVVMRH
                     SGCFDEYRMPIATGIAWFHDCDGGELAYWPQGADGPIERHNVRFNTAMVLDTDSVFHG
                     VQRIADVAVDELPRLRPGMTLDFEGDRSWVVRNGDGDGDEIARYDWPQLRFSVSWKAY
                     CFESDQERDAWREHSDDLTLDMILDRLVDDMRARGRVSGDVARDADLGLALIDEYVRF
                     PSTTSSTR"
     gene            complement(1579..2625)
                     /locus_tag="VFX21_07545"
     CDS             complement(1579..2625)
                     /locus_tag="VFX21_07545"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX5095848.1"
                     /translation="MLAEVHVVTSDGLHDLDRALAPFSADALVLIVPPAAMVRTEALL
                     TRYTLLDGDVVVAAACGPEPGTDPDAHPLVNTPYRFVAPALMGPVAAIRDMQVTSPAD
                     LTAHYLAGADLELDVGAQLFLVRDGTGTDAVCVDGKVVATATGTRPAVVIGDPVPDES
                     GDDLVRLLAYMPRPGKARMIAPEILRVPFWEPEQCATVIRESEAVNAWGSDPDDPVPG
                     LEVSLATISPVLFEHVERHLEERVVPALREFWPEFAWTGVHDAFVIKYAAGSEATSLA
                     LHHDVAQLSGSVRLNTGYEGGALEFPRQDWSNADMAIGELVVWPSLVTHPHRGAPLTR
                     GVKYALTIWIALPG"
     gene            2806..>3050
                     /locus_tag="VFX21_07550"
     CDS             2806..>3050
                     /locus_tag="VFX21_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5095849.1"
                     /translation="MAVDRTNAQQAFDAWQARGTTIDTATGRVFMVDVPAQHDAGLTP
                     MFILHGFPTCSYDWVHVLPAFSRDRRVVLLDFPGFGLS"
BASE COUNT          506 a         1036 c         1019 g          489 t
ORIGIN      
        1 aggtgtcatc gacccgctta gccgcacgcg ccttcaaacc atcgagcagc gcctggatcg
       61 caccgagttc gcgcaccaac tccaacgatg cacgcccatg caaggacgag gcgtcgaagt
      121 ctcgagcgag ctcctcgagc aacgaacgaa ccttccccac ccccacgagc atgagctaat
      181 gctacccgaa cgtgtgttcg atcgtcaaga gatttctcag gcggattcga gaaaatcgcc
      241 ggcccgcggc ccgacgcgcg cgtcacacga cccaacgctc ttctcgttgc gtcgtttcgt
      301 ttgtcgttaa ggcagccgac ggatctcgat gatgcggctc gtgcggaacg acgcgacgcg
      361 gcaggcgaac gcggagccga ggtggccgga gccgccgttg cgttcgaaga aggtcgtcag
      421 cggtccttct tgttcgtacg cgtcggcgtc gtgtacgagc gtgttggtgt cgtcgtcgaa
      481 gtgcaccgag aagctcacac gcggattgta cgacgcgcgc gttgtggatg actagtagat
      541 caacgtgtgg atgacgttgt ggatggaaag cgaacgtact cgtcgatcaa cgcgagaccg
      601 aggtccgcgt cgcgcgcgac atcgcccgac acgcggccac gcgcgcgcat gtcgtcgacc
      661 agacgatcga ggatcatgtc gagcgtgaga tcgtcggagt gctcgcgcca cgcgtcgcgc
      721 tcttgatcag actcgaagca gtacgccttc cacgacaccg agaaccgcaa ctgcggccag
      781 tcgtagcgcg cgatctcgtc accgtcaccg tcgccgttgc gcaccaccca ggagcggtcg
      841 ccttcgaagt cgagtgtcat gccgggtcgc aggcgcggca gctcgtcgac cgcgacgtcc
      901 gcgatgcgct gcacgccgtg gaacaccgag tcggtgtcga gcaccatcgc ggtgttgaag
      961 cgcacgttgt ggcgctcgat cgggccgtcg gccccctgag gccagtacgc cagctccccg
     1021 ccgtcgcagt cgtggaacca tgcaatgccc gtcgcgatgg gcatgcggta ctcgtcgaag
     1081 cagccggagt ggcgcatgac gacgagcagc cactgcggga tcaccttgcg gttcgcgccg
     1141 cggaactcgg gcacatcggt gtggaccgcg agctcctggc ccgggatcat caagttcgca
     1201 tacgcgatcg cgggctcgat cacgggccgg ccgtgaatcg cctgcgcggc ttcgacgaac
     1261 ccagggtggt gcaggaacgg ctcgatgccg tcgatgcgcg cggtctgccc gtacgcgtac
     1321 tcctcacgga agtagctcgt gcggaccgcg agcgcggaaa ccggctcgtc ggcacccgtc
     1381 gatccgccgg tgcgcaggaa gttggtcagc gcgtcgtggc gttgcgcgag cccgcgcccg
     1441 atgtcggtct cgagctgctc gtgctcgagg tacatccggt accggccgaa ccgctcgcac
     1501 agctcgacca tcgcgtccgc gcccgccgcg tcgaacaacg gatccagcag gacgaccggc
     1561 tcatgcatgc attgcacgct atcccgggag ggcaatccag atcgtcaggg cgtacttcac
     1621 gccgcgggtg agaggcgcgc cgcgatgggg gtgcgtcacg agcgacggcc acacgacgag
     1681 ctcgccgatt gccatgtcgg cgttggacca gtcctgccgt gggaactcca gcgcgccgcc
     1741 ctcgtagccc gtgttgaggc gcaccgagcc gctgagctgc gcgacatcgt gatgcagcgc
     1801 gagtgacgtc gcttcgcttc ccgccgcgta cttgatgacg aatgcgtcgt gcacgccggt
     1861 ccatgcgaac tcgggccaga actcgcgcag tgccggcacg acgcgctcct ccaggtggcg
     1921 ttcgacgtgc tcgaacagca ccggactgat cgtcgcgagc gacacctcca ggcccggaac
     1981 cggatcgtcg ggatcgctcc cccaggcgtt caccgcttcg ctctcccgga tgaccgtcgc
     2041 gcactgctcg ggttcccaga acggcacccg caggatctcc ggcgcgatca tccgggcctt
     2101 gcccggtcgc ggcatgtacg caagcagccg cacgaggtcg tcacccgact cgtcgggcac
     2161 cggatcgccg atgacgaccg cgggccgggt gccggtggcg gtcgcgacga ccttgccgtc
     2221 gacgcacacc gcgtcggtgc cggtgccgtc gcggacgagg aagagttgcg cgccgacgtc
     2281 gagctcgaga tcggcaccgg cgagatagtg cgcggtcaga tccgcgggcg atgtgacctg
     2341 catgtcccgg atcgctgcga ccggacccat gagcgccggt gcgacgaagc ggtagggcgt
     2401 gttgaccaag gggtgtgcat cggggtcggt acccggttcg ggaccgcacg cggccgcgac
     2461 gacgacgtcg ccgtcgagca acgtgtagcg cgtgaggagc gcctccgtgc gcaccatcgc
     2521 cgccggcggg acgatcagga ccagcgcgtc ggcggagaac ggtgccagcg cgcgatcgag
     2581 gtcgtgcaac ccgtcgctgg tgacgacgtg gacttccgca agcacggtgt gagcttcgcg
     2641 cgtgccgtcg ccgcatactc gcaacgtggt gggtgtcgac cattcgaatc acaacatcca
     2701 gtaccacgcc gagattctcc gcaccctgcc gcccgcctgc cggcacgtca tgttccgctc
     2761 ctcgctcgtg tggacgaggc cgggcgcgag ttagcctgcc gcaccgtggc agtggaccgg
     2821 acgaacgcgc agcaggcctt cgacgcatgg caagcgcgcg gcacgacgat cgacacggcc
     2881 accggtcggg tgttcatggt cgacgttccc gcgcagcacg acgccgggct gacaccaatg
     2941 ttcatcctgc acggcttccc gacctgctcc tacgactggg tacacgtgct cccggcgttc
     3001 agccgcgacc ggcgcgtcgt gctgctcgac ttcccgggct tcggcctgtc
//
LOCUS       DASUGA010000382         2515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1870322, whole genome shotgun sequence.
ACCESSION   DASUGA010000382 DASUGA010000000
VERSION     DASUGA010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870322"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VFX21_07555"
     CDS             complement(<1..798)
                     /locus_tag="VFX21_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095850.1"
                     /translation="MAPSARGSRPALGAADVLIVAGAVALLVGTRFPFYELDNALSLE
                     WSAWSNAWSLFPVALLGALYGCVPAAALVRARARRRPWTGTESGACVLLGVACLLTVL
                     ASWLRDVRLSTVVLATGPGAWMMFTGATASVAGSWLHYDAAVRDAPPDRPPVRSHVGP
                     AVLVVWLGAAVVLVASFLTVFELPTVGGETVAASAWSRNFSPPLFLLPVVWALLLAAL
                     VSIPLLPRRLRPRTILAVERTDVEFGLSAIAVVTMVGLTVGVPGFRAF"
     gene            862..1422
                     /gene="purN"
                     /locus_tag="VFX21_07560"
     CDS             862..1422
                     /gene="purN"
                     /locus_tag="VFX21_07560"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012086254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX5095851.1"
                     /translation="MRVGVLASGSGTILRALVERDLPIVVAVFDRPCGAIDVAAAHGV
                     ATEVVERTDFSPQFDREAYTHEVIDALRRHDIDLVAIAGFGTILSKPFVDEYGGRAVN
                     THPALLPAFKGWHAVRDALEFGVKVSGCTVHLVAEDVDSGPILAQAAVPVLDDDTEAT
                     LHERIKEVERQLYPDVLEKLIKDRQS"
     gene            1419..>2515
                     /gene="purH"
                     /locus_tag="VFX21_07565"
     CDS             1419..>2515
                     /gene="purH"
                     /locus_tag="VFX21_07565"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX5095852.1"
                     /translation="MRTTITRALLSVHDKTLLVDFARGLHELGVELVSSGGTASAIEA
                     ADIPVTRVEDVTGSPEMLDGRVKTLHPKIHGGLLADLGNESHRADLERQGIRPFDLVV
                     SNLYPFLESPGIETIDIGGPAMVRASAKNFAWVTVVTSPVQYDVLLDELRANQVTVSD
                     ETRRAFALEAFAHTSAYDAAIVQWLQGDELLPKYLTFSLERTNEVLRYGENPHQQAAR
                     YKRQGATSWIDHVTQHSGLALSYLNLYDTEAAWRLAFDLGADPACAIIKHANPCGVAV
                     AASLDVAYERALECDERSAFGGIVACNRPIDHATVERMVAGPQADVVIAPSYDEGVVE
                     ALVAKRRNTRILAARKPDAERLDVRQISGGFL"
BASE COUNT          452 a          880 c          862 g          321 t
ORIGIN      
        1 gaaggcgcgg aagccgggca cgccgaccgt gaggcccacc atcgtgacga cggcgatcgc
       61 cgacagcccg aactccacgt cggtccgctc gaccgcgagg atcgtgcgcg ggcgcaggcg
      121 gcgagggagc aacgggatcg agacgagcgc ggccagcagc agcgcccaca cgaccggcag
      181 caagaacaac ggcggcgaga agttgcggct ccacgcgctc gcggcgacgg tctcgccgcc
      241 gacagtgggc agctcgaaca cggtgaggaa cgacgcgacc agcacgacgg cggccccgag
      301 ccacacgacg aggaccgcgg gcccgacgtg gctgcgcacc ggcggccggt cgggcggcgc
      361 atcgcggacc gcggcgtcgt agtgcaacca cgagcccgcc accgacgccg tcgcgccggt
      421 gaacatcatc cacgcccccg gcccggtcgc gagcacgacg gtgctcagac gtacgtcacg
      481 cagccaagac gcgagcaccg tgagcaggca cgcgaccccc agcagcacac acgcgccgga
      541 ctccgtgccc gtccacggcc ggcggcgggc acgcgcccgc acgagcgcgg ccgcgggcac
      601 gcatccgtag agcgcgccca acaacgcgac cgggaagagg ctccacgcgt tcgaccaggc
      661 gctccattcg agcgacagcg cgttgtcgag ctcgtagaac ggaaaacggg tcccgaccag
      721 gagcgcaacg gcgcccgcga cgatgagcac gtcggccgcg cccagcgcgg gccggctccc
      781 gcgcgcggat ggggccacga cccgaaagtc tggcaaagct cactcgggcg cccaaggacc
      841 cgccccacta cggtggcgat catgcgggtc ggagtcctgg catcaggcag tggaacgatc
      901 ctgcgcgcgc tcgtcgaacg cgatctgccg atcgtcgtcg cggtgttcga ccgcccgtgc
      961 ggtgcgatcg atgtcgccgc cgcgcacggt gtcgcgaccg aagtcgtcga gcgcaccgac
     1021 ttctcaccac agttcgatcg cgaggcgtac acccacgaag tcatcgacgc gctgcgacgc
     1081 cacgacatcg atctggtggc gatcgccgga ttcggcacga ttctgtcgaa gccgttcgtc
     1141 gacgaatacg gcggccgcgc cgtcaacacg caccccgcgt tgctgcccgc gttcaagggc
     1201 tggcacgcgg tgcgcgacgc gctcgagttc ggtgtgaagg tgagcgggtg cacggtgcac
     1261 ctcgtggccg aagacgtcga ctccgggccg atcctcgcgc aggccgcggt acccgtcctc
     1321 gacgacgaca ccgaagcgac attgcacgaa cgcatcaagg aagtagagcg ccagctctat
     1381 cccgacgtac tggagaagct catcaaagac cggcagtcat gaggacgacg atcacgcgcg
     1441 cgttgttgtc ggtgcacgac aagacgttgc tggtcgactt cgcgcgcggg ctccacgagt
     1501 tgggcgtcga gctggtctcg tcgggtggca ccgcgagtgc gatcgaagcg gctgacatcc
     1561 cggtcacgcg cgtcgaggac gtcaccgggt cgccggagat gctcgacggg cgggtgaaga
     1621 cgctgcaccc gaagatccac ggcgggttgc tcgccgacct cggcaacgaa tcccaccggg
     1681 ccgacctgga acgccaggga atccggccgt tcgacctggt cgtgtcgaac ctctatccct
     1741 tcctcgagtc gcccggcatc gagaccatcg acatcggcgg cccggccatg gtgcgggcct
     1801 cggccaagaa ctttgcgtgg gtcaccgtcg tgacgagccc ggtgcagtac gacgtgctgc
     1861 tcgacgagct gcgcgccaac caggtcacgg tgtcggacga gacgcgccgc gcgttcgcac
     1921 tcgaagcgtt cgcgcacacc agcgcgtacg acgccgcgat cgtgcaatgg ctgcaaggcg
     1981 atgagttgct gccgaagtac ctcacattct cactcgaacg tacgaacgag gtattgcggt
     2041 acggcgagaa cccgcaccag caagccgcgc ggtacaagcg gcagggcgcg acgagctgga
     2101 tcgaccacgt cacacaacac agcggtctcg cgctctcgta cttgaacctg tacgacaccg
     2161 aagccgcgtg gcggctcgcg ttcgatctcg gcgcagatcc cgcgtgcgcg atcatcaagc
     2221 acgcgaatcc gtgcggcgtc gcggtcgcgg cctcgctcga cgtcgcgtac gagcgcgcgc
     2281 tggagtgcga cgagcggtcg gcgttcggcg gcatcgtcgc gtgcaaccgt ccgatcgacc
     2341 acgcgacggt cgagcgcatg gtcgccggcc cgcaagccga cgtcgtcatc gcgccgagct
     2401 acgacgaggg cgtcgtggag gcgttggtcg ccaagcgcag gaacacgcgc atcctcgcgg
     2461 cgcgcaagcc ggatgcggaa cgcctcgatg tccgccagat ctccgggggg ttcct
//
LOCUS       DASUGA010000383         2525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1120587, whole genome shotgun sequence.
ACCESSION   DASUGA010000383 DASUGA010000000
VERSION     DASUGA010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120587"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..1284)
                     /gene="argF"
                     /locus_tag="VFX21_07570"
     CDS             complement(142..1284)
                     /gene="argF"
                     /locus_tag="VFX21_07570"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX5095853.1"
                     /translation="MNTKRRLLELDDMTPARVTRVLDRSVAWKADPAAVPQVLAGKAV
                     AAVFQKPSARTRTSVEVAVATLGGHPIYIREEEVGIDRRETAEDVARTLASMCCLIAA
                     RVFEHETLVRMDNAVDVPVVNLLSDTGHPVQALADLLTLQETFGSLDGCRVAYVGDGN
                     NVAASLAFAAALAGIEVVVASPSGFELDADVVDRARNLGATVELVGDPYDAVQGADCV
                     YADVWTSMGQEEEREARLRAFTGWTIDAALMKAANDDAIFLHCLPAHRGEEVAAEVID
                     GPQSRVWQQAGNRMDAVRALFVDMLEAGSTDGHPGQAATAAPHPAHPRRATGLESEPA
                     RRAARGRRRRRDTGDGQPRSRRAGRGEGADSRRHDGLRHPRLHARR"
     gene            complement(1281..2465)
                     /locus_tag="VFX21_07575"
     CDS             complement(1281..2465)
                     /locus_tag="VFX21_07575"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HEX5095854.1"
                     /translation="MTSLDEIVALDAQYVMQTYGRLPVAFVRGEGTRLWDSDGREYLD
                     FLTGLAVVSLGHAHPEVAAAIADQAATLLHVSNLYYNEHQPHVAARLDALLGGGGQSF
                     FCNSGAEANECAIKLARRYGQLHGGLARHHVVSAYGSFHGRTLTTLAATGQPQKQDTF
                     QPLPQGFRQVAFEDVDALAAALDERVAAVMLEPVQGEGGVNPAPPEYLQAVRQLCDER
                     EVLLIMDEVQTGLGRTGKWFGFEHSGVRPDIVTMAKALGNGVPIGACWARAEVAAAFS
                     PGDHATTYGGQPLATRAARAVLEVMERERIPERAARAGARLDVALQKVPGVVAVRGLG
                     LLVAAELAAGIDAKAIAQQCMDNGLIVNAVTASALRLAPPLLVSDDEIDEAVAILTGV
                     LS"
BASE COUNT          377 a          910 c          858 g          380 t
ORIGIN      
        1 agcgcggcac ggtcgacggc cgacgcgacg acgtgggcgc tgccgggcgg cgtgcgcagc
       61 acgaccatgt tcccgctgtg cgcgacctcg agcacgaact cacccatgag ccggcggagg
      121 tggtcgtcgg agctgccgcg ctcaccgccg cgcgtgtagt cggggatggc gtaggccatc
      181 gtgccgccgg gaatccgcac cttcaccgcg cccagctctt cgagatcgcg gctgaccgtc
      241 gcctgtgtcg cgacgacgcc gtcggcctcg agcagcgcga cgagctggct ctgactcgag
      301 accggttgct cttcgaggat gcgcaggatg cggtgctgcc gttgcggctt gcccagggtg
      361 gccatcagtg ctccccgcct cgagcatgtc gacgaacaac gcccgcacgg catccatccg
      421 gttgccggct tgctgccaga cgcgcgactg cggaccgtcg atcacttcgg cggcgacctc
      481 ttcgccgcgg tgcgcgggca ggcagtgcag gaagatcgca tcgtcgttcg cggccttcat
      541 cagcgcggcg tcgatcgtcc agcccgtgaa cgcacgcagg cgcgcctcgc gttcttcttc
      601 ttggcccatc gacgtccaca catcggcata cacacagtcg gcaccttgga cggcgtcgta
      661 cggatcgccc accagctcga ccgtcgcgcc gaggttgcga gcccggtcga cgacgtcggc
      721 gtcgagctcg aagccggagg gcgacgcgac gacgacctcg ataccggcga gtgccgccgc
      781 gaacgcgagc gacgccgcca cgttgttacc gtcaccgacg taggccacgc ggcaaccgtc
      841 gagtgaaccg aaggtctcct gcagcgtcag caagtcggcc aacgcctgca ccgggtgacc
      901 cgtgtcggag agcaggttga caacggggac gtcgacggcg ttgtccatac ggacgagtgt
      961 ctcgtgctcg aacacgcgcg cagcgatgag gcagcacatg ctcgccagcg tccgggccac
     1021 gtcttcggcg gtctcccgcc ggtcgatgcc gacctcttct tcgcgtatgt agatcgggtg
     1081 gccgccgagc gtcgcgaccg cgacctccac cgaggtgcgc gtccgcgccg acggcttctg
     1141 gaacactgcc gcaacggctt tgccggccag cacctgtggc accgcggccg ggtcggcctt
     1201 ccacgcgacc gagcggtcga ggacgcgcgt cacccgcgcc ggcgtcatgt cgtcgagctc
     1261 gagcaatcgg cgcttcgtgt tcacgacagc acgcccgtca agatcgcgac cgcttcgtcg
     1321 atctcgtcgt cgctgacgag caacggcggc gcgagccgca gcgcgctcgc cgtcaccgcg
     1381 ttcacgatca ggccgttgtc catgcactgt tgcgcgatcg ccttggcgtc gatgcctgcc
     1441 gcgagctccg ccgcgaccag gaggccgagg ccgcgcaccg cgacgacacc aggcaccttc
     1501 tggagtgcga cgtcgagccg ggcgcccgca cgcgccgccc gctcgggaat ccgctcgcgc
     1561 tccatgacct cgagcaccgc gcgcgccgcg cgcgtcgcca gcggctgccc gccataggtc
     1621 gtcgcgtgat cgccggggct gaacgcggcg gcaacctcgg cgcgggccca gcacgcaccg
     1681 atcggcacac cgttgcccaa cgccttggcc atggtcacga tgtcgggccg cacacccgaa
     1741 tgctcgaaac cgaaccactt cccggtccgg ccgaggccgg tctgcacttc gtccatgatc
     1801 agcagcactt cgcgctcgtc gcacagctgc cgcacggcct gcaggtactc cggcggcgcg
     1861 gggttcacgc caccttcgcc ctggaccggt tcgagcatga cggccgcgac gcgctcgtcg
     1921 agcgcggccg cgagcgcgtc gacgtcttcg aacgcgacct ggcggaaccc ttggggcaac
     1981 ggctggaacg tgtcttgttt ctgcggctgg ccggtcgcgg ccaacgtggt gagcgtgcgg
     2041 ccgtggaagg agccgtacgc ggagacgacg tggtggcgcg ccagcccgcc gtgcaattgg
     2101 ccgtaccgtc gcgcgagctt gatcgcgcac tcgttggcct ccgcgcccga gttgcagaag
     2161 aacgactgac caccgccgcc gagcagcgcg tcgagacgcg ccgcgacgtg cggctggtgc
     2221 tcgttgtagt agaggttcga gacgtgcagc agggtcgcgg cctggtccgc gatcgcggcc
     2281 gcgacctcgg ggtgcgcgtg accgagcgac acgaccgcca ggcccgtgag gaagtcgagg
     2341 tactcgcgac cgtcgctgtc ccacagccgc gtgccttcgc cgcgcacgaa cgccaccggc
     2401 aggcggccat aggtctgcat cacgtactgc gcgtcgagcg cgacgatctc gtcgagggac
     2461 gtcatgggtg caccatcgtt cccacacctt ctcgcgtgaa gaattcgagc aacagggcgt
     2521 gcggc
//
LOCUS       DASUGA010000384         2428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1174276, whole genome shotgun sequence.
ACCESSION   DASUGA010000384 DASUGA010000000
VERSION     DASUGA010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174276"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..121
                     /locus_tag="VFX21_07580"
     CDS             <1..121
                     /locus_tag="VFX21_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006724539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5095855.1"
                     /translation="AREWQSATSLGPFTLVGCTVSPPFRFAGFELAPDGWEPG"
     gene            174..1991
                     /locus_tag="VFX21_07585"
     CDS             174..1991
                     /locus_tag="VFX21_07585"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX5095856.1"
                     /translation="MTNVDLVRLAGSRGRISASPTVDPVTAEVIRGGMETICFEMAEY
                     VSRTATTPILNQSNERNATVLDAQGRLAALSVGIPQFMLTSTLPVRFALEFLGVDEFR
                     EGDVFVANDPYHGGGHLPDYNVFAPVFADDPAAPGQRRMVLIASIQCHHGDTGGAVPG
                     GYNVTANDIWGEGVRWPVVKVVDRGVERRDVLYALQVNNRIPSYIGDLRAQIGAAQLG
                     AIRLGEIIDRYSASAVEESVDYMIDYAARRFREEVAAWPDGVYEGDAYVDHDPLGNPD
                     VHIHVKVTVEGDSLTIDFSGSDTRPEIQAWSTYGNTRGYTVGQIAAMMDPEIPKNEGF
                     FEQIKLVVPKGCVLNPDPGKPVSAGTHHPGADVGEVIAVAMQHVLPERAVPQTYKTGI
                     PTIIAGVDPRTGATFTDHSAEVYAGWCNASKGMDAWGALNASFGNLWKATAEINESLY
                     PHIQWSRDYRTDSGGPGQWRGICGSHYEKEVLVDAKVYTYVVGMKYPMPGICGGKNGA
                     PNEMIIRYGSDDPFRVQHTADWIPMQAGDRIMYDYGGGGGWGDPFERDPQAVLDDVLD
                     EYVSVEGARRDYGVVLTGSLENLTLQIDHDATRELRAKR"
     gene            2075..>2428
                     /locus_tag="VFX21_07590"
     CDS             2075..>2428
                     /locus_tag="VFX21_07590"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5095857.1"
                     /translation="MGVDVGGTFTDLICVTPSGEVVLDKTPTTLDDQSTGVMNGLGQL
                     AERFDLTLHDFCASLDIVVHGTTTADNTMIEMNGAATGLLVTAGHRDEIELRRCHKEE
                     IWDPNYPGPTPIARRR"
BASE COUNT          436 a          821 c          810 g          361 t
ORIGIN      
        1 ggcgcgcgag tggcaatccg cgacctcgct cgggccgttt acgctggtgg ggtgcacggt
       61 cagcccaccg ttccggttcg cgggcttcga gctcgccccc gacggctggg agccgggctg
      121 agtacacttc ctgacgccag cgtcaggttt gccgcgtcga ttgggagccc gtcatgacga
      181 acgtcgactt ggttcgcctc gccggaagcc gcggtcggat ctccgcatcg ccgaccgtcg
      241 acccggtcac cgcggaagtc atccgcggtg gcatggagac gatctgcttc gagatggcgg
      301 aatacgtctc gcgcaccgcg acgacaccga tcctcaacca gtcgaacgag cgcaacgcga
      361 cggtgctcga cgcgcagggc cgtctcgccg cgctgtccgt cgggatcccg cagttcatgt
      421 tgacctcgac attgccggtg cggttcgcgc tcgagttcct tggcgtcgac gagttccgcg
      481 agggcgacgt gttcgtcgcc aacgacccgt accacggcgg cgggcacctt cccgactaca
      541 acgtgttcgc gcccgtgttc gccgacgatc ccgcggcgcc cgggcagcgg cggatggtgc
      601 tcatcgcgtc gatccagtgc caccacggcg acaccggcgg cgcggtcccc ggcggttaca
      661 acgtcaccgc caacgacatc tggggcgaag gtgtgcgctg gcccgtcgtg aaggtcgtcg
      721 accgcggcgt cgagcgccgc gatgtcctct acgcgctgca ggtcaacaac cgcatcccgt
      781 cgtacatcgg cgacctgcgc gcgcagatcg gtgccgcgca gctcggcgcg atccggctcg
      841 gcgagatcat cgaccggtac agcgcgagcg cagtcgagga atccgtcgac tacatgatcg
      901 actacgcggc ccgccgcttc cgcgaagagg tggcggcgtg gcccgacggc gtatacgaag
      961 gcgacgcgta cgtcgaccat gacccgctcg gcaatcccga cgtgcacatc cacgtgaagg
     1021 tcacggtcga gggcgactcg ctcaccatcg acttcagcgg ctcggacacg cgccccgaga
     1081 tccaagcgtg gtccacgtac ggcaacacgc gcggctacac ggtcggtcag atcgcggcga
     1141 tgatggaccc cgagatcccg aagaacgaag ggttcttcga gcagatcaag ctcgtcgtgc
     1201 cgaagggttg cgtgttgaac cccgacccgg gcaagccggt gagcgcgggc acgcaccacc
     1261 cgggcgccga cgtcggcgag gtgatcgcgg tcgcgatgca gcacgtactg cctgagcgtg
     1321 cggtgccgca gacgtacaag accggtatcc cgacgatcat cgccggcgtc gatccgcgca
     1381 ccggtgcgac gttcaccgat cactcggccg aggtctacgc cggttggtgc aacgcgtcga
     1441 agggcatgga cgcttggggt gcgctcaacg cgagcttcgg caacctgtgg aaggcgaccg
     1501 cggagatcaa cgagtcgctg tacccgcaca tccagtggag ccgcgactac cgcaccgaca
     1561 gcggcggccc gggtcagtgg cgcggcatct gcggcagcca ctacgagaag gaagtgctcg
     1621 tcgacgccaa ggtctacacg tacgtcgtcg gcatgaagta cccgatgccg ggcatctgcg
     1681 gcggcaagaa cggcgcgccg aacgagatga tcatccgcta cggcagcgat gaccccttcc
     1741 gcgtgcaaca caccgccgac tggatcccga tgcaggccgg cgaccgcatc atgtacgact
     1801 acggcggcgg cggcggttgg ggcgacccat tcgagcggga cccgcaagcc gtgctcgacg
     1861 atgtcctcga cgagtacgtg tccgtcgagg gcgcccgtcg cgactacggc gtcgtcctca
     1921 ccggatcgct ggaaaacctg acgctccaga tcgaccacga cgccacccgc gaacttcgcg
     1981 ccaagcgatg agcgacgacg aggaaacaac cggttttggc gacacagggc gaccgaatcg
     2041 gtcgtcagat gacgccaaaa ctgggaagta ccgggtgggg gtcgacgtcg gggggacgtt
     2101 caccgacctg atctgcgtga caccgagcgg cgaggtcgtg ctcgacaaga cgccgacgac
     2161 actcgacgac cagtcgacgg gcgtgatgaa cgggctcggg caactcgccg agcgcttcga
     2221 cctgacgttg cacgacttct gcgccagcct cgacatcgtc gtccacggca cgacgactgc
     2281 cgacaacacg atgatcgaga tgaacggtgc ggcgacgggc ttgctggtca ctgccggcca
     2341 ccgcgacgag atcgagttgc gccgttgcca caaggaagag atctgggacc cgaactatcc
     2401 cggtcccacg ccgatcgccc gtcggcgc
//
LOCUS       DASUGA010000385         4343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_327094, whole genome shotgun sequence.
ACCESSION   DASUGA010000385 DASUGA010000000
VERSION     DASUGA010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327094"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2279
                     /locus_tag="VFX21_07595"
     CDS             <1..2279
                     /locus_tag="VFX21_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095858.1"
                     /translation="PFTLPAQYSRAECDRRRGDIDLEVRRWLRDSALPNHSFAGYDGL
                     VIVIPLNREVGASVGCTFAGTAFGATMISQGIPWWWTSVHEIGHLMGGPGDVQGLQHG
                     PRYRCTRNGMPIVLATAAECTEALVPEGWPDFDGYDAMSSGPYLFHAVHKYRLGIMPE
                     TNVRTVTDTTTVTLTNSESVIRGATQLIRIPWERFPDGTFRTAVYLELRKRQGKFDDS
                     LPDAAVNGISVRYGAYDGGAPGHTGVVNLLDGNPRSATFDDAQLTPGQSFSNALRQTF
                     VRVNSIDTAAGTASLTITRGTPQTTVRVVDGRLEYSATANTASNVRFESAPGGAVDVT
                     DSLFLAEADSSCSPLGDNRVRCANVTSIAATLGDKNDTATVAVDLPSSLDGGSGNDGL
                     SGGPGLDTFDGGPGDDTISSKDGRVETVTCGDGILDEVAGDVAPGVTMENMTACEWVH
                     DGDFSVARPALSPLAFGGDVYSNNNLGRVHGVYTLTDKSVGYRRNSCFVVTGTWCAMT
                     SLGGYATTSPTVVEAGNRTDVVVGGGGGALYVKHQTCRANGTCDPFTSPWENISKDKA
                     GTTVTTTASPDLARYADPRFGGGPYTLLAVRGQNNALWVRVFDGKGWGLWHNFGGTLL
                     GGPGATWVEAQGGGVGMVEVFGVGTDGALWRNTVQCLATTCLANGWTRQGAPPSGRLV
                     GDVDATSRTAGSVDVVARDDRGYARHRVRTGATWSPYVQLAPTLGVYGPTVTSLGNGM
                     LAGSTPGAWATYVEFLRR"
     gene            2316..3104
                     /locus_tag="VFX21_07600"
     CDS             2316..3104
                     /locus_tag="VFX21_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095859.1"
                     /translation="MERESTTHGRILDEAMKHEVDHHVDPARDMEGLTEEPERVETPD
                     VAPGALSDADIRQRSELAQSLRPSIFPAYRDAIVASAVDEDAPPALLERMQRLPAGVR
                     FHTTEEVWEALGGRHEVRAQEREREHEPGPRPTRRFEFRFDQLHRVLGLPFGVTPGTT
                     HVDVDRERNRFVARFGPWCVETPLTNVRRAEVSGGYFPLKTVGGAHLSFADRGLTFAT
                     NDAEGVCITFEEPVTGIEPRGVIRHPGLTVTVADAQGLVEALRG"
     gene            complement(3140..4009)
                     /locus_tag="VFX21_07605"
     CDS             complement(3140..4009)
                     /locus_tag="VFX21_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020667093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5095860.1"
                     /translation="MRTSIAITNFSWPAPDTIAADLDALAEAADGGGIDTIFVADHVQ
                     QAEPGTERSEPMLEAFTTLSHLAARTERVRLGTMVASVTLRAPALLVKAVTTLDVLSG
                     GRAWFGVGAGYHLPELDDLGVPLPPTGERFDWLEDTVELAHRMWSGDDAAFAGRRLRA
                     GRPIASPSPRTQPHPPILVGGWGKKRTLPLVARCADACNLPDMPDGGELIRQSLRVLA
                     AQCDAHERPFEQIEKTVSTRFSPGETPDDMIERARTLAALGMDHVVVLTRGPWTTDHV
                     AALARAVPYIADL"
     gene            3987..>4343
                     /locus_tag="VFX21_07610"
     CDS             3987..>4343
                     /locus_tag="VFX21_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5095861.1"
                     /translation="MIAMLVRTSRAHTHALRPLPGRPSLRGPVRGNNFLGGLAERSRV
                     PDVTTLIEQDHRAVERLFARYKEDRAMATVRRICDALDAQANAEEAVFYRAVRDEVPD
                     GARIAHRATSDHGRVRD"
BASE COUNT          697 a         1513 c         1501 g          632 t
ORIGIN      
        1 gcccgttcac gcttccggcc cagtactcac gggccgaatg tgatcgcagg cggggcgaca
       61 tcgacctcga agttcggcgc tggctccgtg actcggcgct cccgaaccat tccttcgcgg
      121 gctacgacgg cctcgtcatc gtgatccctc tcaaccggga ggtcggcgcg tcggtcggct
      181 gcacgttcgc aggtaccgcg ttcggcgcaa cgatgatctc gcagggcatt ccgtggtggt
      241 ggacgtctgt gcacgagatc ggtcacctca tgggcgggcc gggcgacgtg caaggcctac
      301 agcacgggcc gcgctatcga tgcacgcgca acggcatgcc gatcgtgctc gctacggcgg
      361 ccgagtgcac cgaagcgctc gtgcccgaag gctggcccga cttcgacggc tacgacgcga
      421 tgagcagcgg gccgtacctc ttccatgccg tccacaagta ccggctcggc atcatgcccg
      481 agaccaatgt gcgcacggtc accgacacca ccacggtcac gctcacgaac agcgagagcg
      541 tgatccgggg cgcgacgcaa ctgatccgca tcccctggga acggtttccg gacgggacgt
      601 tccggacggc cgtgtacctc gagctccgca agcggcaagg gaagttcgac gactcgttgc
      661 cggatgccgc ggtcaacggg atcagtgtgc ggtacggcgc gtacgacggt ggggctcccg
      721 gtcacacggg tgtcgtgaat cttctcgacg gcaacccgcg cagcgcgacg ttcgacgacg
      781 cgcaactcac cccgggccag tcgttcagca acgctctgcg gcagacgttc gtccgggtga
      841 actcgatcga cactgcggcc ggaacggcat cgctgaccat cactcgcggc acgccacaaa
      901 cgacggtgcg cgtcgtcgac ggccggctcg agtacagcgc gaccgcgaac accgcgagca
      961 acgttcgctt cgaaagcgcg cccggtggtg cggtcgacgt gaccgacagc ctgttcctcg
     1021 cggaagccga cagttcgtgc agcccgctcg gcgacaaccg tgtgcgctgc gcgaacgtga
     1081 cgagcattgc cgcgacgctg ggcgacaaga acgacacggc caccgtcgcc gtggacctgc
     1141 cgtcgtcgct cgacggcggc tccgggaacg acggcctgag cggcggaccg ggcctcgaca
     1201 ccttcgacgg agggcccggc gacgacacga tctcgtcgaa ggacggccgg gtggagacgg
     1261 ttacgtgcgg cgacggcatc ctcgacgagg tcgccggcga cgtggcaccc ggcgtgacga
     1321 tggagaacat gacggcgtgc gagtgggtgc acgacggcga cttctccgtc gcgcgcccgg
     1381 ccctctctcc gctcgcgttc ggcggcgacg tgtactccaa caacaacctc gggcgcgtgc
     1441 acggcgtcta cacgctcacg gacaaaagcg tcggctaccg ccgcaacagc tgtttcgtcg
     1501 tcaccggtac gtggtgcgcg atgacgtcgc ttggcgggta cgcaacgacg tcgccgacgg
     1561 tcgtcgaagc cggtaaccgc accgacgtcg tggtgggcgg cggcgggggt gcgctctacg
     1621 tgaagcatca gacgtgccgc gccaacggca cctgtgatcc gttcacgagc ccatgggaga
     1681 acatctcgaa ggacaaggcc gggacgacgg tcacgacgac cgcatctccg gacctcgcgc
     1741 gctacgcgga tccgcgattc ggcggcggcc cctacacgct gctcgccgtg cggggacaga
     1801 acaacgcgct ctgggtacgg gtcttcgacg gcaagggctg gggcctctgg cacaacttcg
     1861 gcggcaccct cctcggcggc ccgggtgcga catgggtcga ggcgcagggc ggcggggtcg
     1921 gcatggtcga ggtcttcggc gtcgggaccg acggcgcgct gtggcgcaac accgtgcagt
     1981 gcctcgccac cacgtgcctc gccaacggct ggacccgtca gggcgcgccg cccagcggcc
     2041 gactcgtcgg tgacgtcgac gcgacgtcac gcaccgccgg ctccgtcgac gtggtcgcgc
     2101 gcgacgatcg cgggtacgca cgacaccgcg tacgaaccgg tgcgacgtgg tcgccgtacg
     2161 tgcaactcgc accgacgctc ggcgtatacg ggccgaccgt gacgtccctc ggcaacggca
     2221 tgctggccgg atccacgccc ggcgcatggg ccacctatgt cgaattcctc cgccgctgag
     2281 caatagcgct tcgcgctcgc gggtacgcgc gcgcgatgga acgtgagagc acgacacacg
     2341 ggcggatcct cgacgaggcg atgaagcacg aggtcgacca tcacgtcgat ccggcccgcg
     2401 acatggaggg gctcaccgaa gagcccgagc gcgtcgaaac tccggacgtc gcgcccggcg
     2461 ctctgtcgga tgcagacatc aggcagcgct cggagctcgc gcagagtctg cgcccgtcga
     2521 tctttcccgc ctaccgcgac gcgatcgtcg cctccgctgt cgacgaggac gcaccgccgg
     2581 cgctgctcga acggatgcag cggctcccgg cgggagtccg gttccacacg acggaggaag
     2641 tgtgggaggc gctcggcggc cggcacgagg tgcgcgcgca ggaacgcgaa cgagaacacg
     2701 agccgggacc gcgaccgacg cgtcgcttcg agttccgctt cgaccagctg caccgcgtgc
     2761 tcgggctgcc cttcggtgtg acgcccggga cgacacacgt cgacgtcgat cgggaacgga
     2821 accgcttcgt cgcgcggttc ggaccgtggt gcgtcgagac gccgctgacg aacgtccggc
     2881 gcgcggaggt cagcggtggc tacttccccc tgaagaccgt cggcggcgcc catctttcgt
     2941 tcgcggaccg cggtctgacc ttcgcgacga acgacgcgga gggtgtttgc atcaccttcg
     3001 aggagcccgt gaccgggatc gaaccgcgcg gcgtcattcg ccaccccgga ctgacggtca
     3061 ccgtcgccga cgcgcagggc ctggtcgagg cgctccgcgg ctaggagtcg tcagaaccct
     3121 cgcgacacac gctccttggt cagaggtccg cgatgtatgg cactgcgcgt gcgagcgcag
     3181 cgacgtggtc ggtggtccac ggcccgcggg tcagcaccac gacgtggtcc attcccaacg
     3241 ccgccaatgt gcgcgcgcgt tcgatcatgt cgtcgggggt ctcgcccggt gagaagcgcg
     3301 tgctcaccgt cttctcgatc tgttcgaagg ggcgctcgtg cgcatcgcac tgcgcggcga
     3361 gcacccgcag gctctggcgg atcagctcgc cgccgtcggg catgtcgggc aggttgcacg
     3421 catccgcgca acgggcgacg agcggcagcg tccgcttctt gccccaccca ccgaccagga
     3481 tcggcgggtg cggctgcgtg cgcgggctcg ggctcgcgat gggccggccg gcacggagtc
     3541 gccggcccgc gaatgccgcg tcgtctcccg accacatgcg gtgcgccagc tccaccgtgt
     3601 cttccaacca gtcgaaacgt tccccggtcg gcggcagcgg tacgcccagg tcgtccagct
     3661 ccggcaggtg gtagcccgcg ccgacaccga accaggcccg gccgccggac agtacgtcga
     3721 gcgttgtcac cgctttcacg agcagcgcgg gcgcacgcaa cgtgaccgac gcgaccatcg
     3781 tgccgagccg cacacgttca gtccgcgcgg ccagatggga gagcgtcgtg aacgcctcca
     3841 acatcggctc cgaccgttcc gttcccggtt cggcctgctg cacgtggtcg gcaacgaaga
     3901 tcgtgtcgat gccacctccg tcggcggcct ccgcgagggc gtcgaggtcc gcggcgatgg
     3961 tgtcgggggc cggccacgag aagttcgtga tcgcgatgct ggtgcgcacg tcacgagcgc
     4021 atacccacgc gcttcgaccg ctacccgggc gcccgagttt gcgcggcccc gtccgcggca
     4081 acaacttcct ggggggcctc gcggaaagga gccgcgtgcc tgatgtgaca acactcatcg
     4141 aacaggacca tcgcgccgtc gagcgactct tcgctcggta caaggaagac cgcgccatgg
     4201 cgaccgtccg ccggatctgt gacgcgctcg atgcgcaggc caacgccgag gaggcggtct
     4261 tctatcgcgc ggtgcgagac gaggtgccgg acggcgcacg gatcgcgcat cgtgccacca
     4321 gcgaccacgg ccgagtgcga gat
//
LOCUS       DASUGA010000386         4239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1094981, whole genome shotgun sequence.
ACCESSION   DASUGA010000386 DASUGA010000000
VERSION     DASUGA010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094981"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VFX21_07615"
     CDS             <1..901
                     /locus_tag="VFX21_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HEX5095862.1"
                     /translation="VHFRDKKIGDDVDINVVARGTPGMSGADLANLVNEAALFAVRRQ
                     ASEVHQEDFDAARDRVIMGLKRESMALSEEEKEIVAYHEGGHAVLAYVLEHADPLHKV
                     TILPTGMALGVTQQLPLEERHIYKREYIADSLVVRLGGRVAEELVFGHQSTGASNDLV
                     GNTELARRMVREWGMSDRVGPMAWGSQGAVFLGDDLMHSRDYSDETARVIDEEVERIL
                     RDEEDRTRKVLTEYRAGLDAIAQALLERETLSADEVAHIVDTAMGRKVGGPRKITHAD
                     GTEEYADEPLEPGLEGDAVGPTW"
     gene            979..1917
                     /locus_tag="VFX21_07620"
     CDS             979..1917
                     /locus_tag="VFX21_07620"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX5095863.1"
                     /translation="MVLAVALLAASCLPPRPVLPAVPKHADAISALTLNVLHGLPCVD
                     NDYCHAPDRAEITAQYVEAMGCPDLVGFQEIGFRQPDVFPPAMSAVCGGRYQLAWPGV
                     AGVDRTMIFTTLPILDQGAVDLAAFPWEAYWVRVDAPMGPVDFLTTHFASSSNNPICP
                     AQNCPPVCPDGIRANQCNALEVVQLLDAQRAGAVMQIVSGDLNARPDSPTYDELRQAG
                     FVDTWLAARRPECKPQNGHGCTSGRDRPENDVDGMDAPTGHYTSRIDYILTRPATGCS
                     LRANAKPVAATPLQEPFNGLYWPSDHAGVWAKISCR"
     gene            complement(1904..2335)
                     /locus_tag="VFX21_07625"
     CDS             complement(1904..2335)
                     /locus_tag="VFX21_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095864.1"
                     /translation="MAARLLVAVVLLVAAVIVALVIERRRRPAAPSQGRDVVPHQLDR
                     NDFPRPDAPWLVVLWSSTACESCAGLIDKLRPLESPDVAVVEVEYQSNKALHARYKIE
                     AAPITQVVDAQGVTQASFTGAFEATELWSIVAALRDAPTDS"
     gene            2400..3812
                     /locus_tag="VFX21_07630"
     CDS             2400..3812
                     /locus_tag="VFX21_07630"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5095865.1"
                     /translation="MSVNSGADKKTVSAWPMWVLGFVIMIDQVDQNIVRGVIPQLQDD
                     FGINDAQIGILLSAFVLVNGLVTVPAGYLADRWNRTRTIGHTVVGWSLITMLTAAAGN
                     FATLVGLRALLGFGQAITEPSAASLLSDYYPTNERGKVFSNQQVMGFLGFGLGIAIGG
                     WVGSNVGWRAAFLVVGPPSLFVALLAYRLREPKRGHGDRLHVGIEDTETEPEEHPKLF
                     EDGFQRFVIDMTRGLRDDFSTIWAIPTLRYALVGVSALLFTVAGIGAWLPQFHERFSD
                     MSQDEATAVVGGLIIFGGIPGVLFGGRLADRFAQRVKGARVVIPAYCIWIGNTIFIIS
                     YLRIPAGLSVFLQLLGIFTITAAIPALRAGMADAVPAHLRGAGFGAFNLVSILFGAAA
                     APFIVGALADIWNLRIAFLIVSPPVYIGAFVLFRARKHLDADAMKIFEAVVRAMEQDQ
                     ERHAAREAAHDSAPDPDSTA"
     gene            complement(3857..>4239)
                     /locus_tag="VFX21_07635"
     CDS             complement(3857..>4239)
                     /locus_tag="VFX21_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095866.1"
                     /translation="LVSRADVNTESGVSTTAGVARTADRWVLARSRVADEHATILSIV
                     NSGATELQASIALVHDGVVEELPELQGITVAPSRPTIVTLSDLSPNDAAVVVTATGPV
                     SVQRTLFGAAEQSTAMGIPERLHR"
BASE COUNT          668 a         1485 c         1428 g          658 t
ORIGIN      
        1 ggtccacttc cgcgacaaga agatcggcga cgacgtcgac atcaacgtcg tcgcgcgcgg
       61 cacgcccggc atgagcggcg ccgacttggc caacctggtc aacgaagccg ccctgttcgc
      121 cgtgcgccgc caggcgtccg aggtccacca ggaagacttc gacgcggctc gcgaccgcgt
      181 gatcatgggt ctcaagcgcg agtcgatggc gttgtcggag gaagagaagg agatcgtcgc
      241 ctaccacgag ggcgggcatg ccgtgctcgc ctacgtgctc gagcacgccg atcccctcca
      301 caaggtgacg atcctgccca cgggcatggc cctcggcgtc acccaacagc tcccgctcga
      361 ggagcgccac atctacaagc gcgagtacat cgccgattcg ttggtcgtac gtctcggtgg
      421 ccgcgtcgcc gaggagctcg tcttcggcca ccagtcgacc ggcgcgtcga acgacctcgt
      481 cggcaacacc gagctcgcgc ggcgcatggt tcgcgagtgg ggcatgtcgg atcgcgtcgg
      541 cccgatggcg tgggggtcgc agggcgccgt gttcctcggc gacgacctca tgcactcgcg
      601 cgactactcg gacgagaccg cacgagtgat cgacgaggaa gtcgagcgga tcctgcgcga
      661 cgaggaagac cggacccgca aggtgctcac cgagtaccgc gccggcctgg acgcgatcgc
      721 ccaggcgctg ctcgagcgcg agacgctcag cgcggacgag gtcgcccaca tcgtcgacac
      781 ggccatgggc cgcaaggtcg gcgggccgcg caagatcacg cacgcggacg gcaccgagga
      841 atacgccgac gagccgctcg aacccggcct cgaaggcgac gccgtcggcc cgacctggta
      901 aggaagagag aaaccggaag gttctctcgc gcgggcggcg tcggtacggt ccggcgcatc
      961 aaggttcgtt ggctgctcgt ggtgctcgcg gtggcattgc tcgccgcgtc gtgcctcccg
     1021 ccgcgcccgg tgttgcccgc ggtgccgaag catgccgacg cgatcagcgc gctgacgctc
     1081 aacgtgctgc acgggctccc gtgcgtcgac aacgactact gccacgcacc cgatcgcgcc
     1141 gagatcaccg cgcaatacgt cgaggcgatg ggctgccccg acctcgtcgg cttccaggag
     1201 atcggcttcc gccaacccga cgtgttcccg cccgcgatga gcgcggtctg cggcggccgg
     1261 taccaactcg cgtggccggg cgtcgccggc gtcgaccgca cgatgatctt cacgacgttg
     1321 ccgatcctcg accagggtgc ggtcgatctg gccgcgtttc cctgggaggc ctactgggtg
     1381 cgcgtcgacg cgccgatggg acccgtcgac tttctcacga cgcacttcgc gagcagcagc
     1441 aacaacccga tctgccccgc gcagaactgc ccgccggtct gtcccgacgg gatccgcgcc
     1501 aaccagtgca acgcgctcga agtcgtgcag ctactcgacg cccagcgtgc cggcgcggtg
     1561 atgcagatcg tcagcggaga cctgaacgcc cggcccgaca gcccgacgta cgacgaacta
     1621 cggcaggccg gcttcgtcga tacgtggctc gcggcgcgcc gccccgagtg caagccgcag
     1681 aacgggcacg gctgcacgag cgggcgcgac cggcccgaga acgacgtcga cggcatggat
     1741 gcaccgaccg gtcactacac cagccgcatc gactacatcc tcacgcgccc cgcgaccggg
     1801 tgctcactac gcgccaacgc gaagccggtc gcggcgacgc cgctgcagga gccgttcaac
     1861 gggttgtact ggcccagcga ccatgcgggt gtctgggcga agatcagctg tcggtaggcg
     1921 cgtcgcgtaa ggcggcgacg atcgaccaga gctccgtcgc ctcgaacgcg ccggtgaacg
     1981 acgcttgcgt gacgccctgc gcgtcgacga cttgggtgat cggcgcggct tcgatcttgt
     2041 agcgcgcgtg cagcgccttg ttcgactggt attcgacttc gacgaccgcg acgtcgggcg
     2101 actcgagcgg ccgcagcttg tcgatcagac ccgcgcagga ctcacacgcg gtcgacgacc
     2161 acagcacgac gagccatggc gcgtcgggcc gcgggaagtc gttgcggtcg agttggtgcg
     2221 ggacgacatc gcgtccttgt gacggtgccg cgggtcggcg gcggcgctcg atcaccagcg
     2281 cgacgatcac cgcggcgacc aacaacacga ccgcgacgag gagccgggca gccatcaggt
     2341 ggacacggta tgcgccctcg atgccaaact ctgagccggg cttccgggcg aggggcgcag
     2401 tgtcggtcaa cagcggtgcg gacaagaaga cggtgtcggc gtggccgatg tgggtgctcg
     2461 gcttcgtcat catgatcgac caggtcgacc agaacatcgt gcgcggcgtc attccgcagc
     2521 tgcaggacga cttcggcatc aacgacgcgc agatcggcat cctgctctcg gcgttcgtgc
     2581 tggtgaacgg tctggtcacc gtgcccgcgg gatacctcgc cgaccgctgg aaccgcacgc
     2641 gcacgatcgg tcacaccgtc gtcgggtgga gcttgatcac gatgctcacg gccgcggccg
     2701 ggaacttcgc cacgctcgtc ggcctgcgcg cgctgctcgg cttcggccag gccatcaccg
     2761 aaccgtcggc cgcgagcctg ctctccgact actaccccac caacgaacgc gggaaggtct
     2821 tctccaacca gcaagtcatg gggttcctcg ggttcgggct cggcatcgcg atcggcggct
     2881 gggtcggctc gaacgtcggc tggcgcgctg cgttcctcgt cgtcgggccg ccgagccttt
     2941 tcgtcgcgtt gctcgcgtac cggttgcggg aacccaagcg cggccacggc gaccgcctgc
     3001 acgtcggcat cgaagacacc gagaccgaac ccgaagagca tccgaagctg ttcgaagacg
     3061 gcttccagcg cttcgtcatc gacatgacgc gtggcctgcg cgacgacttc agcacgatct
     3121 gggcgatccc taccctgcgc tacgcgctcg tcggcgtcag cgcgctgctg ttcacggtcg
     3181 cgggcatcgg cgcgtggctc ccgcagttcc acgagcggtt ctccgacatg agtcaggacg
     3241 aagccaccgc ggtcgtcggt gggctgatca tcttcggcgg cattcccggt gtgctgttcg
     3301 gcggccggct ggcagaccgc ttcgcgcaac gcgtgaaggg cgcgcgcgtc gtcatcccgg
     3361 cctactgcat ctggatcggg aacacgatct tcatcatctc gtacctgcgt atcccggccg
     3421 ggctgtcggt gttcctccag ctgctcggga tcttcacgat caccgcggcc atcccggcgc
     3481 tgcgcgcggg catggccgac gccgtccccg cgcacctgcg cggcgccggg ttcggcgcgt
     3541 tcaacctcgt gtcgatcctg ttcggcgcgg ccgccgcgcc cttcatcgtc ggcgcactcg
     3601 ccgacatctg gaacctgcgg atcgcgttct tgatcgtgtc gccgccggtc tacatcggcg
     3661 cgttcgtcct cttccgcgcc cggaagcacc tcgacgcgga cgcgatgaag atcttcgaag
     3721 cggtcgtccg cgcaatggag caggaccagg aacggcacgc ggcacgcgag gccgcgcacg
     3781 attccgcgcc tgaccccgac tcgacggctt gaaagcgggc gcggattggc cgcatgcggc
     3841 gcagccgtcc tcgcccttag cggtgcagcc gctcggggat gcccatcgcg gtcgactgct
     3901 cggccgcacc gaacaacgtg cgctgcaccg agaccggtcc ggtggcggtg acgacgactg
     3961 ccgcgtcgtt cggtgagaga tccgacagcg tgacgatcgt cggtcggctc ggcgcgacgg
     4021 tgatgccttg caactccggc agctcttcga cgacaccgtc gtgcacgagt gcgatgctcg
     4081 cctgcagttc ggtcgcgccg ctgttgacga tcgacaagat cgtcgcgtgc tcgtcggcga
     4141 cgcgcgagcg agccaacacc cagcggtcgg cggtgcgcgc gacacccgcg gtcgtcgata
     4201 ctccggactc cgtgttgacg tcggcccggc tcaccagct
//
LOCUS       DASUGA010000387         5006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_288640, whole genome shotgun sequence.
ACCESSION   DASUGA010000387 DASUGA010000000
VERSION     DASUGA010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288640"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VFX21_07640"
     CDS             <1..263
                     /locus_tag="VFX21_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095867.1"
                     /translation="SRLPTPPAGTAGTATIVALCGEDEAVDAASRALASRPDVLVVVA
                     GRPRPGLRDGIACAFRGCDCLEVLGALLAAATRGATPAGGER"
     gene            260..2404
                     /gene="scpA"
                     /locus_tag="VFX21_07645"
     CDS             260..2404
                     /gene="scpA"
                     /locus_tag="VFX21_07645"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018000385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HEX5095868.1"
                     /translation="MSEAPDFTRLGYGFDEFGNDAGADRPSPTFPTAEQIDVRSRPTR
                     ADLEGLEHLDYLPGLPPFGRGPYATMYSRRPWTVRQYAGFSTAEESNAFYRRNLAMGQ
                     QGLSIAFDLATHRGYDSDHERVRGDVGMAGVAIDSVEDMKILFDGIPLEKMSVSMTMN
                     GAVIPILALYVVAAEEQGVATEQLAGTIQNDILKEFMVRNTYIYPPEPSMRIVADIIQ
                     YTAQNMPKFNSISISGYHMQEAGATAVQELGFTLADGLEYVRYATARGLSVDEFAGRL
                     SFFFAIGMNFFMEVAKLRAARLLWARIMKQQGAQKPNSMMLRTHCQTSGVSLTEQDPY
                     NNVVRTAYEALAAVLGGTQSLHTNSFDEAIALPSDFAARIARNTQLILAEETGVTHVV
                     DPLGGSYYVEALTASLANEAWKLIEEVEALGGMTKAVESGMPKLRIEESAARRQAAID
                     RGDETIVGVNKYRLENERPVDVREIDNTEVRRQQLERLERVKATRDNARVEAALEALT
                     NGAASDGNLLQLSIEATRARATVGEISAAMEKVFGRHRAEIRAISGVYGGAYADDEEF
                     QRIRKEVDEFADGEGRRPRILVAKLGQDGHDRGAKVIATAFADLGFDVDIGALFQTPE
                     EAAREAIDNDVHVVGVSSQAAGHKTLVPQLVEALKAQGAGEIVVVVGGVIPASDYDYL
                     RAAGVSAVFGPGSNIPRAASEILGIIRNKRAA"
     gene            2504..3481
                     /gene="meaB"
                     /locus_tag="VFX21_07650"
     CDS             2504..3481
                     /gene="meaB"
                     /locus_tag="VFX21_07650"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX5095869.1"
                     /translation="MSDAQEIVDGVRSNDRRALARAITLVESTRADHRADAAALLELC
                     MPYTGGALRVGITGAPGAGKSTFIEALGLHLVEQGRRLAVLAVDPSSTRSGGSILGDK
                     TRMEMLARDERAFIRPSPSGGTLGGVARRTREAMLVCEAAGFDVVLVETVGVGQSEVA
                     VASMVDVFVLLLAPGGGDELQGVKRGIVELADVVVVNKADGDLAPAAQRTAADYQNAL
                     HLVRGPGDGPAVRVLTSSALAGTGIAEMWAAVGETVAAARTDGSLERRRREQARQWMW
                     SEVSDTLVEALRDQARAREMQDVESEVASGRMSPTAGARELLDRFSWPC"
     gene            complement(3465..3662)
                     /locus_tag="VFX21_07655"
     CDS             complement(3465..3662)
                     /locus_tag="VFX21_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095870.1"
                     /translation="MSRDTDTAAEHEGQLRLIPGGPRARRWALDERTRQVGRAGVAEA
                     REILRHARPPQPRERNLSKAS"
     gene            3779..>5006
                     /locus_tag="VFX21_07660"
     CDS             3779..>5006
                     /locus_tag="VFX21_07660"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEX5095871.1"
                     /translation="MKPWKQSVAVVFALALFVTACGGGDDDDDSSSDTTKASSSEDDA
                     EGAITSMDDAESAVIRIVAKGTFAEPSEEGVASYNEVTGVGSGSGFIIDPSGIAVTNN
                     HVVTGAASLEVYVGDEKDDPVSAKVLGVSECDDLAVIDLAGDGYPFLKWSDDPPKTGR
                     DVRAAGFPLGDPEFTLTSGIISKAEANGDTPWASTPNIIEHDAAIQPGNSGGPLLDAK
                     TAAVVGVDYATNDPGTGQNQFFAIGAELAQDVVEQMAQGENVLALGVNGQAIYQEDDG
                     VVGVWVSSVESGSPASEVGLHGGDIIERIEGLPLATDGTMKDYCDILRSHDPTDTLAV
                     QVLRFADAARYRGEFNGDELTPSESLASTVEEETGSTLAAGTAYDYVPVTDDSGTLYV
                     EVPSAWSDVATSPYVLE"
BASE COUNT          805 a         1732 c         1750 g          719 t
ORIGIN      
        1 cgtcccgact gcccaccccg cctgcgggca ccgcgggcac cgcgacgatc gtcgcgctgt
       61 gcggcgagga cgaggcggtg gacgccgcct cgcgcgcgct ggcgtcccgt cccgacgtcc
      121 tcgtggtcgt ggccgggcgg ccccgtcccg ggctgcgcga cgggatcgcg tgcgcgttcc
      181 gcggctgtga ctgcctcgag gtcctcggtg cgctgctcgc cgcggcgacg cgtggcgcca
      241 cgcccgcggg aggcgaacga tgagcgaagc acccgacttc acccggctcg gctacggctt
      301 cgacgagttc ggcaacgacg ccggagcgga ccggccctcg cccaccttcc ccaccgcgga
      361 acagatcgat gtgcggtcga ggccgactcg cgccgatctc gagggactgg agcacctcga
      421 ctacctgccg gggctgccgc cgttcgggcg cggcccctac gcgacgatgt actcgcgtcg
      481 cccgtggacc gtgcgtcagt acgccgggtt ctccaccgcc gaggagtcca acgcgttcta
      541 tcgccgcaac ctggcgatgg gacagcaggg cctctccatc gcgttcgatc tcgccacgca
      601 ccgcggctac gacagcgacc acgaacgcgt gcgcggagac gtcggcatgg caggcgtcgc
      661 gatcgactcg gtcgaggaca tgaagatcct cttcgacggc atcccgctcg agaagatgtc
      721 ggtgtcgatg accatgaacg gtgccgtgat cccgatcctc gcgctctacg tcgtggcggc
      781 ggaggaacag ggcgtcgcca ccgagcagct cgccgggacc atccagaacg acatcctcaa
      841 ggagttcatg gtccgcaaca cctacatcta tccgcccgaa ccgagcatgc ggatcgtcgc
      901 cgacatcatc cagtacacgg cgcagaacat gccgaagttc aactcgatct cgatctccgg
      961 ctaccacatg caagaagcgg gcgcgaccgc ggtgcaggag ctcggcttca cgctcgccga
     1021 tggtctcgag tacgtgcggt acgcgaccgc gcggggcctc agcgtcgacg agttcgcggg
     1081 ccgcctgtcg ttcttcttcg cgatcggcat gaacttcttc atggaagtcg cgaagctgcg
     1141 cgcggcgcgg ttgctgtggg cgcgcatcat gaagcagcag ggcgcgcaga agccgaactc
     1201 gatgatgctg cgcacgcact gccagacgtc gggcgtgtcg ctcaccgagc aagatccgta
     1261 caacaacgtc gtgcgcaccg catacgaagc gctcgcggct gtgctcggcg gcacgcagag
     1321 cctgcacacg aacagcttcg acgaagcgat cgcgctgccc tccgacttcg cggcgcgcat
     1381 cgcacgcaac acacagttga tcctcgccga ggaaaccggc gtcacccacg ttgtcgaccc
     1441 gctcggcggc tcgtactacg tcgaagcgct caccgcgagc ctcgccaacg aggcgtggaa
     1501 gctgatcgaa gaggtcgagg cgctgggcgg catgacgaag gccgtcgagt cgggcatgcc
     1561 gaagctgcgc atcgaggagt cggcggcgcg ccgccaggcc gcgatcgacc gcggcgacga
     1621 gacgatcgtc ggcgtcaaca agtaccgcct cgagaacgaa cggccggtcg acgtccgcga
     1681 gatcgacaac accgaggtgc gccgccagca gctcgaacgg ctcgagcggg tgaaggcgac
     1741 ccgcgacaac gcgcgcgtcg aagccgcact cgaagcgctc acgaacggcg ccgcgtccga
     1801 cggcaacctg ttgcagctct cgatcgaggc gacgcgtgcc cgcgcgaccg tcggtgagat
     1861 ctccgccgcg atggagaagg tcttcggccg tcaccgcgcc gagatccgcg ccatctccgg
     1921 tgtgtacggc ggtgcatacg ccgacgacga ggagttccag cgcatccgca aagaagtcga
     1981 cgagttcgcc gacggtgaag gccggcgccc gcgcatcctc gtggccaagc tcggccagga
     2041 cggtcacgac cgcggcgcga aggtcatcgc gaccgcgttc gccgacctcg gcttcgacgt
     2101 cgacatcggc gcgctgttcc agacgcccga agaagcggcg cgcgaagcga tcgacaacga
     2161 cgtgcacgtc gtcggcgtgt cgagtcaggc cgcggggcac aagacgctcg tgccgcaact
     2221 tgtcgaagca ctgaaggcac agggtgcagg cgagatcgtg gtcgtcgtcg gcggcgtcat
     2281 ccccgcgtcc gactatgact acttgcgcgc ggcgggcgtg agcgcggtct tcggtcccgg
     2341 cagcaacatc ccgcgtgcgg cctcggagat cctggggatc attcgcaaca aacgcgccgc
     2401 ataattcttt gtcgctccgg tcgtgctcgc gtgaggcggc ccggagtgtt ggcccgccct
     2461 ccgctccggc gagctcgctt cgctcgccgc taccgtgcga cgtgtgtccg acgcgcaaga
     2521 aatagtcgac ggtgtacgaa gcaacgatcg ccgggcactg gcgcgggcga tcacgctcgt
     2581 cgagtcgacg cgtgccgacc accgtgccga cgcggccgcg ctgctcgagt tgtgcatgcc
     2641 gtataccggt ggcgcgctgc gcgtcggcat caccggcgcg cccggcgcgg gcaagtcgac
     2701 gttcatcgag gcactcgggc tgcacctcgt cgagcagggc cggcggctcg cggtgctcgc
     2761 ggtcgacccg tcgagcaccc gcagcggcgg ttcgatcctc ggcgacaaga cccgcatgga
     2821 gatgctggcg cgcgacgagc gcgcgttcat ccgcccgtcg ccgtcgggtg gcacgctcgg
     2881 cggtgtcgcc cgccgcaccc gtgaggcgat gctcgtctgc gaagcggccg ggttcgacgt
     2941 cgtgctcgtc gagacagtgg gcgtcgggca gtcggaggtc gcggtcgcgt cgatggtcga
     3001 cgtgttcgtg ctgttgctcg cgccgggcgg cggtgacgag ctgcaaggcg tgaagcgcgg
     3061 gatcgtcgag ctggccgacg tcgtcgtggt caacaaggcc gacggcgacc tcgcgccggc
     3121 cgcgcagcgc accgccgccg actaccagaa cgcgctgcac ctcgtacgcg gcccgggcga
     3181 cggccccgcg gtgcgcgtgc tcacgagctc ggcgctcgcg ggcacgggca tcgcggagat
     3241 gtgggccgcg gtcggcgaga cggtcgccgc cgcccgcacc gacggttcgc tcgaacggcg
     3301 gcggcgtgag caggcgcgcc agtggatgtg gtcggaggtc agcgacaccc tcgtcgaggc
     3361 cctgcgcgat caggcgcggg cgcgcgagat gcaggatgtg gaaagcgaag tggcatcagg
     3421 gcgcatgtca ccgacggccg gcgcccggga gctgctcgac cggttcagct ggccttgctg
     3481 aggttgcgct cgcgcggctg cggcgggcgg gcgtgacgga ggatctcgcg ggcctcggcc
     3541 acaccggccc gaccgacctg gcgagtccgc tcgtccagcg cccagcgccg ggcccgcgga
     3601 ccgcccggga tcagccggag ctggccctcg tgctcggcgg cggtgtcggt gtccctactc
     3661 acacgttgag tatccccatc gggtgagaca actacaacgc gaccccccgc aattcacagg
     3721 gtgttccaag gtcccgtgcg agactgccgc caagtttttc gcatgcggag ggaccccgat
     3781 gaagccatgg aagcagtcgg tcgcggtcgt gttcgcgctc gcgctgttcg tcactgcctg
     3841 cggcggaggt gacgacgacg acgactcgtc gtccgacacg acgaaggcgt cctcgagcga
     3901 ggacgatgcc gagggggcga tcacctcgat ggacgacgcc gagtcggccg tcatccggat
     3961 cgtcgccaag ggcacgttcg cggagccgtc cgaggaaggc gtcgcgtcct acaacgaggt
     4021 caccggcgtc gggtcggggt cgggcttcat catcgacccg tcgggcatcg ccgtcacgaa
     4081 caaccacgtc gtcaccggcg cggcctcgct cgaggtgtac gtcggcgacg agaaggacga
     4141 ccccgtcagc gccaaggtgc tcggcgtctc cgagtgcgac gacctcgccg tcatcgacct
     4201 cgccggcgac ggctacccgt tcctgaagtg gagcgacgac ccgcccaaga cgggacgcga
     4261 cgtgcgcgcg gccggcttcc cgctcggtga tccggagttc acgctgacga gcggcatcat
     4321 ctcgaaggcc gaggccaacg gcgacacacc gtgggcgtcg acgcccaaca tcatcgagca
     4381 cgacgctgcg atccaacccg gcaactccgg tggaccgctg ctcgacgcga agaccgcggc
     4441 agtcgtcggt gtcgactacg caacgaacga ccccggtacg gggcagaacc agttcttcgc
     4501 gatcggtgcg gagctcgcgc aggacgtcgt cgagcagatg gcccagggcg agaacgtgct
     4561 cgcgctcggc gtcaacggcc aggccatcta ccaagaggac gacggcgtcg tgggcgtctg
     4621 ggtgtcgtcg gtcgagtccg ggagtcccgc cagcgaggtc gggctccacg gtggcgacat
     4681 catcgagcgc atcgagggcc tgccgctcgc gaccgacggc acgatgaagg actactgcga
     4741 catcctgcgc tcgcacgatc ccaccgacac gttggcggtg caggtgctgc ggttcgcgga
     4801 tgcggcgcgc taccgcggcg agttcaacgg cgacgagctg acgccgtcgg agtcgctcgc
     4861 gtccaccgtc gaggaagaaa ccggcagcac gctcgccgcc ggcaccgcct acgactacgt
     4921 gccggtgacc gacgactccg gcacgttgta cgtcgaagtg ccgtccgcgt ggtcggacgt
     4981 cgccacctcg ccgtatgtgc tcgagg
//
LOCUS       DASUGA010000388         3753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393 k141_8528,
            whole genome shotgun sequence.
ACCESSION   DASUGA010000388 DASUGA010000000
VERSION     DASUGA010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8528"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="VFX21_07665"
     CDS             complement(<1..1296)
                     /locus_tag="VFX21_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095872.1"
                     /translation="MSTWGDEPQGGQPGHQPWQQSWQQPSAPPQPPAAWSPSPPSWAP
                     PPSRPPQQKLWPAFLVIALLAAATGATYWFVARDDSPSYPSEWDSRVTDLVAFVEEER
                     NLSFHHPVEIEFLDDDAWEKEMTTDPGTVSEDDAQDAEDFVAEMRALGLMSGDVDLLE
                     ETNDLQVSGVLALYDFEAKEIRVHAAPDAELTIGTRGTLVHELTHVIQDQNFDLDEMA
                     ANAGDDDIVFDALTEGDASNVERAWYDKLSDDDKAKYDAEFESAGDELDAVDAPPVLE
                     TIFGAPYALGPPFAQLLAAESTAALDDAYEDLPSGDEVVFSPEHYLAGDTREIVAKPA
                     VPEGAREIDDGAFGPLGWYIMLSERIDAHQAFTAARGFGGDNYVEYEDHDETCVRIRY
                     TGESGLETVAMKGAIDAWIASLPTPFARVTQDGDELLFES"
     gene            1337..2587
                     /locus_tag="VFX21_07670"
     CDS             1337..2587
                     /locus_tag="VFX21_07670"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5095873.1"
                     /translation="MTLVSFAPFRHRSYARLWTGAFVSNIGTWMQMVAIAAYVTDVTG
                     KATWTGLVAAADFIPIALLAPIGGALADRAPRRLLLVTTSLVQAGFAAALTLLFVTGD
                     PAPALVTLCVFGGGVASALGFPAYQAMLPDLVPEEDLPGAISLSSAQYNLGRIVGPAL
                     AGIVIAVGGYGWALGLNAASFFAVVVVVLSLTLPPPPNANAGVTIRAAIAEGVRFVRG
                     DAGLRVAFSAMCVNTLLAAPFIALVPAMAQNVFDAGSRGTSILVTAQGIGAVLMAFML
                     NSFVQRFGTGRTVATLLTVLPAALVLYAGAPHLWSSAIALFVVGFLYLGTLSSFFTIS
                     QLRAPAALRGRVLSVNNLVLGSLYPLGALIQGRLADAVGLRVTTAAAAAILAFVVIIA
                     RVLRPGLVDVIDAGVDIGPSAVAT"
     gene            complement(2597..3028)
                     /locus_tag="VFX21_07675"
     CDS             complement(2597..3028)
                     /locus_tag="VFX21_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095874.1"
                     /translation="MPVREITRTLNQPPPVVTERASRPALDKRWMVPVFGTLVAAFAA
                     VQFFTPEPVHPDMAPTLLDQLFSTILTGCIFFTAYGFVQRSPRMGYFGAAGFAWTLLF
                     GIASCPMSGHHMYGMWWGAQMALGLAAVATSTVAAVAHARR"
     gene            3132..>3753
                     /locus_tag="VFX21_07680"
     CDS             3132..>3753
                     /locus_tag="VFX21_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5095875.1"
                     /translation="MERRGATALLTLNRPEARNAISPEVSRTMAGLLDQIEADDEIRA
                     VVVTGAGEVFCAGADLKVVASGNALDIARGKGGFAGIVTRDFPKPLIAAVNGPALAGG
                     FEIVLSCDLVVAASHARFGIPEAKRGLLAAAGGLIRLPKRVSLAVALELAMTGDPIDA
                     DRALALGLVNRVVAADQVIDAALALADAIAANSPIAVRCARRLVREA"
BASE COUNT          512 a         1341 c         1263 g          637 t
ORIGIN      
        1 cgactcgaac aggagctcgt cgccgtcttg ggtcacgcgc gcgaacggcg tcggcagcga
       61 cgcgatccac gcgtcgatcg cacccttcat cgcgaccgtc tcgaggcctg attcgccggt
      121 gtagcggatg cgcacacagg tctcgtcgtg gtcctcgtac tcgacgtagt tgtcgccgcc
      181 gaagccccgc gcagcggtga atgcctggtg cgcgtcgatg cgctcggaca gcatgatgta
      241 ccaacccaac ggtccgaacg caccgtcgtc gatctcgcgc gcgccttccg gcaccgcggg
      301 cttggccacg atctctcgcg tgtcgccggc gaggtagtgc tcgggtgaga acacgacctc
      361 gtcgccgctc ggcaggtctt cgtacgcgtc gtcgagcgca gccgtgctct cggccgcaag
      421 caactgggcg aacggtggac cgagcgcata cggcgcaccg aagatggtct ccaggaccgg
      481 cggcgcatcg accgcgtcga gctcgtcgcc cgcgctctcg aactcggcgt cgtacttcgc
      541 cttgtcgtcg tcggacagct tgtcgtacca cgcgcgctcg acgttcgacg cgtcgccttc
      601 ggtcagggcg tcgaacacga tgtcgtcgtc gccggcgttc gcggccatct cgtcgagatc
      661 gaagttctga tcctggatga catgcgtcag ctcgtgcacc aacgtgccgc gcgtcccgat
      721 cgtcagttcc gcgtcgggag cggcatggac ccggatctcc ttcgcctcga agtcgtagag
      781 cgcgagcacc ccgctgacct gcaggtcgtt cgtctcttcg aggagatcca cgtcacccga
      841 catcagcccg agtgcgcgca tctccgcgac gaagtcttcg gcgtcctgcg cgtcatcctc
      901 gctgacggtg cccgggtcgg tcgtcatctc cttctcccac gcgtcgtcgt cgaggaactc
      961 gatctcgacg gggtggtgga acgagaggtt gcgttcctct tcgacgaacg caacgaggtc
     1021 ggtgacgcgc gagtcccact cgctcggata cgagggcgaa tcgtcgcggg cgacgaacca
     1081 ataggtcgcg ccggtggcgg cggcgagcag cgcgatgacc aagaacgcgg gccagagctt
     1141 ctgctgtggc ggccgcgacg gcggcggtgc ccacgaaggc gggctcgggc tccacgccgc
     1201 gggcggttgc gggggtgcgg agggctgctg ccacgactgc tgccacggct ggtgccccgg
     1261 ttgtccgccc tgcggctcat cgccccacgt cgacatcggt tcattgtgcc agcacagcac
     1321 tagcgtcgaa tccactgtga cgcttgtctc gttcgctccg ttccgccacc ggtcgtacgc
     1381 gcggttgtgg acgggcgcct tcgtctcgaa catcggtacg tggatgcaga tggtcgccat
     1441 cgcggcctac gtcaccgacg tgaccggcaa ggcgacgtgg acggggctcg tcgcggccgc
     1501 cgacttcatc ccgatcgcat tgctcgcgcc gatcggcggc gcgctcgcgg accgcgcgcc
     1561 gcggcggttg ctcttggtca cgacgtcgct cgtacaggcc ggcttcgccg ccgcgctcac
     1621 cttgttgttc gtcaccggcg atcctgcacc cgcgctcgtc acgctgtgcg tgttcggcgg
     1681 cggcgtcgcg tctgcgctcg ggttccccgc ctaccaggcg atgttgcccg acctcgtgcc
     1741 cgaagaagac ctgcccggcg ccatcagcct gtcgtccgcg cagtacaacc tcggacgcat
     1801 cgtcgggccc gcgctcgccg gcatcgtcat cgccgtcggc ggttacgggt gggcattggg
     1861 cctgaacgcg gccagcttct tcgccgttgt cgtcgttgtc ctgtcgctga cgctcccgcc
     1921 accgccgaac gccaacgccg gtgtcacgat ccgcgccgcc atcgccgaag gtgtgcggtt
     1981 cgtacgcggt gatgccggcc tgcgcgtcgc gttctcggcg atgtgcgtga acacgctgct
     2041 ggccgcgccg ttcatcgcgc tcgtgcccgc gatggcccag aacgtgttcg acgcgggctc
     2101 acgcggcacc tccatcctcg tcaccgcgca gggcatcggc gcggtactga tggcgttcat
     2161 gctcaacagc ttcgtgcaac gcttcggcac cggacgcacg gtcgccaccc tgctcaccgt
     2221 cctgcccgcc gcgctcgttc tctacgcagg cgcgccgcac ttgtggtcgt cggccatcgc
     2281 gctgttcgtc gtcggcttcc tctacctcgg gacgctgtcg tcgttcttca cgatctcgca
     2341 gttgcgcgcg cccgcggcac tgcgcggccg cgtgttgtcg gtgaacaacc tcgtgctcgg
     2401 ctcgctgtac ccgctcggcg cattgatcca gggccggctc gccgacgcgg tcggcttgcg
     2461 cgtcacgacg gccgccgccg ccgcgatcct ggccttcgtc gtcatcatcg cccgagtgct
     2521 caggccggga ctcgtcgacg tcatcgacgc cggcgtcgac atcggaccct ccgccgtcgc
     2581 gacctgagcc cgaggatcag cggcgagcgt gcgcgaccgc ggcgaccgtg ctggtcgcca
     2641 ccgcggccag accgagcgcc atctgcgcac cccaccacat gccgtacatg tggtgaccac
     2701 tcatcgggca cgacgcgatg ccgaagagca gcgtccacgc gaacccggcc gcaccgaagt
     2761 accccatgcg cgggctgcgc tgcacgaacc cgtacgcggt gaagaagatg cagccggtca
     2821 ggatcgtcga gaacaactgg tcgagcagcg tcggcgccat gtcgggatgc accggctcgg
     2881 gcgtgaagaa ctgcacggcc gcgaacgcgg cgacgagcgt cccgaacacc gggaccatcc
     2941 agcgcttgtc gagcgcgggg cggctcgccc gttccgtgac gacgggcggc ggctggttca
     3001 gcgtgcgggt gatctcacgt accggcatcc catgcctcct tgctcgcgca tccttacgcg
     3061 tatcagatgt cggcctccga gcgacagcac tcaagccccg agtacggtcc aacttcatgg
     3121 cagttgtgga aatggaacgg cggggcgcga ccgccctgct cacgctgaac cggcccgagg
     3181 cccgcaatgc gatcagtccg gaggtcagcc ggacgatggc cggcctgctc gaccagatcg
     3241 aggccgacga cgagatccgg gcggtcgtcg tgaccggcgc gggcgaggtg ttctgcgcgg
     3301 gcgccgacct caaagtggtt gcgtcgggca atgccctcga catcgcccgg ggcaagggcg
     3361 ggttcgcagg gatcgtgacc cgtgacttcc cgaagccgct gatcgcggcc gtgaacggcc
     3421 cggcgctcgc gggcgggttc gagatcgtgc tgtcgtgcga cctggtcgtc gccgcgagcc
     3481 acgcccggtt cgggatcccc gaggccaagc ggggcctgct ggccgcggcc ggagggctga
     3541 tccgcctgcc caagcgggtg tcgctggcgg tcgcgctcga gttggccatg accggcgacc
     3601 cgatcgacgc cgaccgagcc ctcgccctgg gactcgtcaa ccgggtcgtc gccgccgacc
     3661 aggtcatcga tgcggcgctg gccctggccg acgccatcgc cgcgaactcg cccatcgcgg
     3721 tgcgctgcgc ccgccggctg gtgcgggagg cgg
//
LOCUS       DASUGA010000389         4352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_420375, whole genome shotgun sequence.
ACCESSION   DASUGA010000389 DASUGA010000000
VERSION     DASUGA010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420375"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1182)
                     /locus_tag="VFX21_07685"
     CDS             complement(154..1182)
                     /locus_tag="VFX21_07685"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX5095876.1"
                     /translation="MRIGLMGATGMVGTEMLRILEERNFPVDELRPYASARSEGRKLP
                     FAGGEVTCQVLTEKCFDGLDLVIVDVDDPIALEWAPIAAAAGAKVVDNSAAFRMDPDV
                     PLVIAEVNPQDLRNMPKGIASCPNCTTMIPLTALAPLHKAARLDRMVVSSYQSVSGAG
                     QAGSHELDAQWTKLDGRMADLSRAGTFDGYLVDGEQWPKPIAGNVIPLAGSVKEEGYT
                     SEEWKMVRETRKIFHDDDIKCAVTCVRVPVYVGHAVSANLRFHRPMTKAEAVEILRDA
                     PGVALIDNGDGIPTPHDAAGIDPVLVGRIREDISEPGSLNIWITGDNLRKGAALNAVQ
                     MAEVLLNQ"
     gene            complement(1189..2430)
                     /locus_tag="VFX21_07690"
     CDS             complement(1189..2430)
                     /locus_tag="VFX21_07690"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX5095877.1"
                     /translation="MSGLVVQKFGGTSVADPDRIKAVADHVVRTRRGGDDVVVVVSAM
                     GKTTDDLVRLAGDVTRAPGGREMDMLLTSGERISMALLSMAIEDLGQPAMSFTGSQAG
                     ILTDTEHGKAKIIEVRGDRIREALAGGKVAIVAGFQGVSTERDITTLGRGGSDTTAVA
                     LAAALGADVCEIYTDVEGVYTADPRIVPKAHKLARVSFDEMLEMAATGGRVLALRSVE
                     FARNHGVKIHVRSSFTWAPGTWVTEEDASMDAKTESMEQAIISGVTHDTSEAKFTIEQ
                     VPDRPGVAATVFRDLAEAGINVDMIVQNVSTAGHTDISFTAPRADVPKTERCMEKVLV
                     DTEASGFRTDEKIGRVSLVGAGMKTHPGIAAKMFETLATEGVNIEMISTSTIRISCVV
                     AEEDVERAVQALHSAFGLDRE"
     gene            2500..3342
                     /locus_tag="VFX21_07695"
     CDS             2500..3342
                     /locus_tag="VFX21_07695"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5095878.1"
                     /translation="MPSADKRQRKKENARAAREAREAAEKRQKRMKGIRNAVIAAAVV
                     IAGIVLFSFILNKNDDDSASDTTTTTTPTESTTPGATVSPAAVEKVSCEKVTPEKPKK
                     DTSQSEPKMSIDENKTYTATIETSCGNIKVELDAKNAPKGVNNFVTLAKSGFYDGLTF
                     HRAVTDFVIQGGDPNGDGSGNPGYSTVTELPADGYPLGTLAWAKTGTAPAGSAGSQFF
                     VVTSDHPSALEQKQDDKYQYGAFGKVTEGIEIAKKIEGFDDKSSENKVSQPVVIKKIT
                     ISES"
     gene            complement(3343..4029)
                     /locus_tag="VFX21_07700"
     CDS             complement(3343..4029)
                     /locus_tag="VFX21_07700"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEX5095879.1"
                     /translation="MLALLGGDEWTKPCEAFDRQLLEHAGTSEVLVLPAAAAFENPED
                     AVARASTYFAGLGAKVTNMNVLTRRDADAQANVAAARTAKFVYIADGSPLHLRSVLKG
                     SEFYDALVYAYRERGAVIAASGAGATVLCDPMVDPRGGAYTVGLGIVSGLAIFPYHGT
                     TADHLRERSIDLLSDNASLVGVDEATALIRQPDGGFSVAGAGRITVYRKGVKPKTVKS
                     GGTLEFAPTP"
     gene            complement(4061..>4352)
                     /locus_tag="VFX21_07705"
     CDS             complement(4061..>4352)
                     /locus_tag="VFX21_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095880.1"
                     /translation="STTQAPAAARDRTGSNQQTAQEGSGSGNGSAGADALTGPNGEKL
                     SEAQILAKVCPDVKDTGDDAAAQGSGDAKNYGSVAKRFTQALAKKCEARDKP"
BASE COUNT          746 a         1474 c         1388 g          744 t
ORIGIN      
        1 acgctgtggg tcggcgccat tggcggcctg ctcgcgattc cgtgggtgct ggccaagccg
       61 gtgctcgagc tgcgcgagat tcccgcgggc atcggcgccg acgaaacgcc gctgccgaat
      121 tcccagatct cagagtttcg cgtcgacgac tgactactgg ttgagcagta cttcggccat
      181 ctgcaccgcg ttcaaggcgg cgccctttcg aaggttgtcg cccgtgatcc agatgttcag
      241 cgagccgggc tcggagatgt cttcgcgaat ccggccgacg aggaccggat cgatgccggc
      301 cgcgtcgtga ggcgtgggga tgccgtcacc gttgtcgatc aacgcgacgc cgggcgcgtc
      361 gcgcaggatc tcgaccgctt ccgccttcgt catcggccgg tggaaccgca ggttcgcaga
      421 cacggcatgg ccgacgtaca ccggtacacg cacgcacgtc accgcacact tgatgtcgtc
      481 gtcgtgaaag atcttgcgcg tctcgcgcac catcttccat tcctctgacg tgtagccctc
      541 ttccttcacc gagcccgcga gcggaatcac gttgcccgcg atcggcttcg gccactgctc
      601 accgtcgacg aggtaaccgt cgaacgtgcc ggcgcgggag aggtcggcca tacggccgtc
      661 gagcttggtc cactgcgcgt cgagttcgtg cgaaccggcc tggccggctc ccgacaccga
      721 ctggtacgac gacacgacca tgcgatcgag ccgcgccgcc ttgtgcaacg gcgccaacgc
      781 ggtgagcgga atcatcgtcg tgcagttggg acacgatgca atgcccttcg gcatgttccg
      841 caagtcttgc ggattcactt ctgcgatcac caacggcacg tcgggatcca tacggaacgc
      901 agccgagttg tcgacgacct tcgcaccggc ggcggccgcg atcggcgccc actccaacgc
      961 gatcggatcg tcgacgtcga cgatgacgag atcgagaccg tcgaaacact tctccgtcag
     1021 cacctggcac gtgacctcgc cgcctgcgaa cggcagcttg cggccctccg agcgggcgga
     1081 cgcgtacggc cgcagctcgt cgaccggaaa gttgcgctcc tcgagaatgc gcaacatctc
     1141 ggtgcccacc atccccgtgg cgcccatcag accgattcgc atgaggtatt actccctatc
     1201 gaggccgaag gcggagtgca gcgcctgcac cgctcgctcg acgtcttctt cggcgaccac
     1261 gcacgagatc cgaatggtcg acgtgctgat catttcaata ttgacacctt cggtcgccag
     1321 cgtttcgaac attttcgcgg cgataccggg gtgggtcttc atgccggcgc cgacaagcga
     1381 aacgcggccg atcttctcgt cggtgcggaa gccactcgct tccgtgtcga cgagcacctt
     1441 ctccatgcag cgctcggtct tcgggacgtc ggcgcgtggc gcggtgaacg aaatgtcggt
     1501 gtgaccggcc gtcgacacgt tctgcacgat catgtcgacg ttgatgccgg cttcggcgag
     1561 atcgcggaac acggtggccg cgacacccgg acggtcggga acctgttcga tcgtgaactt
     1621 cgcttccgag gtgtcgtgcg tgacacctga gatgatcgcc tgttccatcg attcggtctt
     1681 ggcgtccatc gacgcgtcct cctcggtcac ccacgttccg ggcgcccatg tgaaactcga
     1741 acgcacgtga atcttcacgc cgtggttacg cgcgaactcg acggatcgca gcgcgagtac
     1801 gcgccctccg gtcgcggcca tttccaacat ttcgtcgaac gacacgcgcg ccaacttgtg
     1861 cgccttcggc acaatgcggg ggtcggcggt gtacacgccc tcgacatcgg tatagatctc
     1921 gcacacgtcg gcgccgagcg ccgccgcgag cgcgaccgca gtcgtgtcgg agccgccgcg
     1981 gccgagcgtc gtgatatcgc gctcggtcga cacgccttga aagccggcaa cgatcgcgac
     2041 cttgccaccg gccagcgcct cgcggatgcg gtcgccgcgc acctcgatga tcttcgcctt
     2101 gccgtgttcc gtgtcggtga ggattccggc ctgcgaaccg gtgaagctca tcgcgggctg
     2161 gcccagatcc tcgatcgcca tcgaaagcag cgccatcgag atgcgttcgc ccgaagtcag
     2221 gagcatgtcc atctcgcggc cgcccggcgc tcgggtgacg tcaccggcca ggcgcacgag
     2281 gtcgtcggtg gtcttgccca tcgccgacac cacgaccacg acgtcgtcgc ccccgcgccg
     2341 ggtgcgcacg acgtggtccg cgaccgcctt gatgcggtcg ggatcggcga ccgaagtgcc
     2401 gccgaatttc tggactacca ggcctgacat cgggagacag gctaccggcg aggggtcccg
     2461 cccccggaag agattgaggg cgctgtaccc tctgcggccg tgccgagtgc ggacaagcgt
     2521 cagcggaaga aggaaaacgc ccgggcggcg cgcgaagcgc gcgaagccgc ggagaagcgc
     2581 cagaaacgca tgaagggcat ccgcaacgcg gtcatcgccg cggccgtcgt catcgcgggc
     2641 atcgtcctct tcagcttcat cctcaacaag aacgacgacg actcggcgtc cgacaccacg
     2701 accacgacga cgccgaccga gagcaccaca ccgggagcga ccgtgtcacc ggccgcggtg
     2761 gagaaggtgt cgtgcgagaa ggtcacgccc gagaagccca agaaggacac gtcgcagtcc
     2821 gaaccgaaga tgtcgatcga cgagaacaag acgtacaccg cgacgatcga gacgagctgc
     2881 ggaaacatca aggtcgagct cgacgcgaag aacgcaccga aaggcgtgaa caacttcgtc
     2941 acgctcgcca agtcgggctt ctacgacggt ctcacgttcc accgcgccgt gaccgacttc
     3001 gtcatccagg gcggcgaccc caacggtgac ggttcgggga accccggcta ctcgacggtg
     3061 accgagctgc cggctgacgg ctatccgctc ggcacgctcg cgtgggcgaa gacgggaacg
     3121 gcaccggccg gctccgccgg ttcgcagttc ttcgtcgtga cctcggacca cccgagcgcg
     3181 ctcgaacaga agcaggacga caagtatcag tacggcgcgt tcggcaaggt gaccgagggc
     3241 atcgagatcg cgaaaaagat cgaaggcttc gacgacaagt cgtcggaaaa caaggtctcc
     3301 cagccggtcg tcatcaagaa gatcacgatc agcgagtcgt gatcacggcg tcggtgcgaa
     3361 ctccagcgtg ccgccgctct tgacggtctt tggtttcacg cccttgcggt agacggtgat
     3421 gcggcccgcg cccgcgacgc tgaagccgcc gtcgggttga cggatcaacg cagttgcctc
     3481 gtcgacgccg acaagtgatg cgttgtcgct cagcaagtcg atcgagcgct cgcgcaggtg
     3541 gtcggccgtc gtgccgtggt acgggaagat cgccaagccc gacacgatgc cgagcccgac
     3601 ggtgtaggcg ccgccgcgcg gatcgaccat cgggtcgcac aacaccgttg cgcccgcacc
     3661 cgacgccgcg atcactgcgc cccgttcgcg gtacgcgtag acgagggcgt cgtagaactc
     3721 gctgcccttc agcaccgagc gcaagtgcag tggcgagccg tcggcgatgt acacgaactt
     3781 cgcggttcgc gcggcggcga cgttggcttg tgcgtcggcg tcgcggcgcg tgagcacgtt
     3841 catgttggtg accttcgcgc cgaggcccgc aaagtacgtc gatgcgcgcg cgaccgcgtc
     3901 ctcggggttt tcgaacgcgg cagccgcggg cagcaccagc acctccgagg tgccggcgtg
     3961 ttccagcagc tgacgatcaa acgcttcgca cggcttcgtc cactcgtcgc cgccgagcag
     4021 cgccagcatg cctgttgttc gtttcatccc tcgcccacag tcacggtttg tctcgagcct
     4081 cgcacttctt cgcaagcgcc tgagtgaatc gcttcgccac gctgccgtag ttcttcgcgt
     4141 caccgcttcc ctgcgcggcg gcatcgtcgc cggtgtcctt cacgtcggga cagaccttcg
     4201 ccaggatctg ggcctcgctc agcttctcgc cgttgggacc ggtcagcgcg tcggcaccgg
     4261 cgctgccatt gccggatccg ctgccctcct gcgccgtctg ttggttgctc ccggtccggt
     4321 cgcgagctgc cgcaggggct tgggtcgtcg ac
//
LOCUS       DASUGA010000390         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1007741, whole genome shotgun sequence.
ACCESSION   DASUGA010000390 DASUGA010000000
VERSION     DASUGA010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007741"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VFX21_07710"
     CDS             complement(<1..392)
                     /locus_tag="VFX21_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095881.1"
                     /translation="MTTCVIGASGDLGRRVCAGLAALDVPYRPLARRGDATYADLADP
                     GSLAAAFAGGTRLFLVSSPVPEQVQLETNAIEAAERARFERLVKISNSPIAGLDTGVH
                     GNHRAIERRLAASSVPSTLVRPSFFTTV"
     gene            421..1149
                     /locus_tag="VFX21_07715"
     CDS             421..1149
                     /locus_tag="VFX21_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX5095882.1"
                     /translation="MRVPQPGPGIGRALTVQQQLACALRILAPEWQENLSGHLTWAND
                     DGSMWVNPWGVWWQEVRASQIIRVDADGNVVEGDLDVTPVVAMHTELHRARPDATLIV
                     HNHPYYATLLSIMGETPRIVHQNSCIFDGELAFVDEYTFTEGVDAASSLADAVGKASG
                     VLLAHHGALVTAPTVEELCYKATTFERMCRFTYDILAAGREPVEFPASERPGMQAVMK
                     GSPLQAYWDGALRDLLARSPEVLD"
     gene            1155..>2124
                     /locus_tag="VFX21_07720"
     CDS             1155..>2124
                     /locus_tag="VFX21_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5095883.1"
                     /translation="MSIDDLAASPAFGGGKGRRETITWLPEPEPRERRYTVFSVDDHV
                     VEPPDAFAGRFPAKFADAAPRVVATDDGGEAWVWQGQLLPNVGFNAVVGRPSTEYGFE
                     PTRFDEMRRGAWDVHARAADMDVDGVAASLCFPSFLPGFVGQRLTMWPDDDELALVAM
                     RAYNDWHFDAWCGAEPGRFVPNQITYLRDPVVAAAEVRRNAARGFRALTFSEAPDKLG
                     LPTIHSGYWDPLFAAAEETETVVCLHVGSSGTSPTTSDDAPPEIPAVLFGAYGMYSAV
                     DWLYSKIPVRFPNIKICLSEGGIGWVAGILDRLDHCYKYQLGYLPTW"
BASE COUNT          323 a          702 c          769 g          330 t
ORIGIN      
        1 atgaccgtgg tgaagaacga cggccggacg agcgtcgagg gcacgctcga tgccgcgagc
       61 cgccgttcga tggcgcggtg gttgccgtgc acgcctgtgt cgagcccggc gatcggactg
      121 ttggagatct tgacgaggcg ctcgaagcgg gcgcgctcgg ccgcctcgat cgcgttcgtc
      181 tcgagctgta cttgctcggg taccggcgac gagacgagga acagccgcgt cccacccgcg
      241 aatgcggcag cgagtgaccc gggatcggcg agatcggcgt acgtcgcatc accgcggcgg
      301 gcgagcgggc ggtacggcac atcgagcgcg gcgaggccgg cgcagacgcg ccgaccgaga
      361 tcgccggacg caccaatgac gcacgtcgtc accggccgag tctggcacta ccgtcgtgac
      421 gtgcgagtac cgcagccggg gccgggcatc ggccgagcgc tcactgtcca gcagcagctc
      481 gcgtgcgcgc tgcgcatcct cgccccggag tggcaggaga atctgtcggg gcacctcacg
      541 tgggccaacg acgacggctc catgtgggtc aacccgtggg gtgtgtggtg gcaggaggtg
      601 cgcgcgtcgc agatcatccg cgtcgacgcc gacggcaacg tcgtcgaggg cgacctcgac
      661 gtcacaccgg tcgtcgcgat gcacaccgaa ctgcaccgcg cccgccccga tgcgacgctg
      721 atcgtgcaca atcacccgta ctacgcgacg ttgctgagca tcatgggcga gacgccgcgc
      781 atcgtgcacc agaactcgtg catcttcgac ggggagctcg cgttcgtcga cgagtacacc
      841 ttcaccgaag gcgtcgacgc ggcatcgtcg ctggcggacg cggtcggcaa ggcgtcaggg
      901 gtgttgctcg cgcatcacgg cgcactcgtg accgcgccga cggtcgagga gctgtgttac
      961 aaggcgacga cgttcgaacg catgtgccgc ttcacgtacg acatcctcgc cgccggacgc
     1021 gagccggtgg agttccccgc ctcggagcgg cccggcatgc aagcggtgat gaagggatcg
     1081 ccgttgcagg cgtactggga cggcgcgctg cgcgacctgc tggcgcgttc accggaggtc
     1141 ctcgactagt ggggatgtcg atcgacgatc tcgcggcgtc gcctgcgttc ggcggcggca
     1201 agggccggcg ggagacgatc acgtggttgc cggaaccgga accacgcgag cgccggtaca
     1261 cggtcttctc ggtcgacgac cacgtcgtcg aaccgccgga cgcgttcgcg ggccggttcc
     1321 cggcaaagtt cgccgatgcg gcgccgcgtg tggtcgccac cgacgatggc ggcgaggcct
     1381 gggtgtggca gggccagttg ctgcccaatg tcgggttcaa cgcggtcgtc ggccgaccgt
     1441 cgaccgagta cgggttcgag ccgacgcgct tcgacgagat gcggcgcggc gcgtgggacg
     1501 tgcacgcgcg cgcggccgac atggacgtcg acggtgtcgc cgcgtcactg tgcttcccgt
     1561 cgtttctgcc cgggttcgtc gggcagcggt tgacgatgtg gcccgacgac gacgagctcg
     1621 cgctggttgc gatgcgcgcg tacaacgact ggcacttcga cgcgtggtgc ggggcggagc
     1681 cgggacggtt cgtgcccaac cagatcacct acttgcgaga cccggtcgtc gccgcggcgg
     1741 aagtgcggcg caacgcggcg cgcggattcc gggcgctgac gttctcggag gcgcccgaca
     1801 agctcggcct gcccaccatc cactccggct actgggatcc gctgttcgcg gccgcggaag
     1861 agaccgagac ggtcgtgtgc ctgcacgtcg gttcgtcggg cacgtcgccg acgacatcgg
     1921 acgacgcgcc gcccgagatc cccgccgtgt tgttcggcgc gtacggcatg tactccgcgg
     1981 tcgactggct gtactcgaag atcccggtgc gcttcccgaa catcaagatc tgcttgtcgg
     2041 agggcggcat cggttgggtc gcggggatcc tcgaccggct cgaccactgc tacaagtacc
     2101 aactcggtta cctgccgacc tggc
//
LOCUS       DASUGA010000391         3504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_947956, whole genome shotgun sequence.
ACCESSION   DASUGA010000391 DASUGA010000000
VERSION     DASUGA010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947956"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VFX21_07725"
     CDS             complement(<1..316)
                     /locus_tag="VFX21_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095884.1"
                     /translation="MTAAAEPVTPTRRRTWTVPVALAAGAAIVRLPIFFATRHVGFDE
                     SVYGASIIGMRHGQWPYKDLFSAMGPLHFVLLRAGDLLGFQTRYSPRIAPVLAGIGIT
                     LAT"
     gene            445..2091
                     /locus_tag="VFX21_07730"
     CDS             445..2091
                     /locus_tag="VFX21_07730"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX5095885.1"
                     /translation="MAKHVFVTGGVASSLGKGLTASSLGRLLKTRGLRVTMQKLDPYI
                     NVDPGTMNPFQHGEVFVTDDKGETDLDLGHYERFVDVALTRKSNATTGSIYQAVLAKE
                     RKGEYLGETVQVIPHITNEIKSRINALATEDVDVVITEIGGTVGDIEILPFLEAIRQF
                     RNEVGRENVFYVHVTLMPYIGPAGEQKTKPTQHSVTELRARGIQPDAIVCRSDRPIPT
                     RLKEKISALCDVPVEGVVSAIDADILYEIPLVLHDEGLDSYLCRVLHLDDREPDLTEW
                     RALVDRVRAADQEVHIGLVGKYINLPDAYLSVVEALKHGGYASGAKVVVDWIAADDTE
                     GLLAEGRLAGLDGIVVPGGFGVRGIEGKIAAAGYAREHGIPYLGLCLGLHAATIEFAR
                     NVCGLAGANSSEFDSQSPHPVIDLLDEQRDVVDMGGTMRLGAYPAKLEAGTIVRKVYG
                     DEVVYERHRHRYELNNRYRQVFEENGMRLSGTSPDGLLVEFIELVDHPFFVATQAHPE
                     FKSRPNRPHPLFAGFVEAARERAEGRAPRLPIDEIETVTL"
     gene            2176..3120
                     /gene="xerD"
                     /locus_tag="VFX21_07735"
     CDS             2176..3120
                     /gene="xerD"
                     /locus_tag="VFX21_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX5095886.1"
                     /translation="MDELDKLLAEHYEWLSVERGLAKNSLAAYRRDLNRYTEFLRARN
                     ITDPAEVGEHTVHEFVEYLQALTDDDGRPLLAPSSRARAIVSVRSFHRFCAVEGFLPS
                     DPTEDVGAPRVPQGIPKALDEEEVTALLGAVTGDSALAQRDRALLETLYATGIRISEA
                     VGLDQGDVDLEDGLLRVLGKGDKERIVPVGRSARRALTAYFGDGFLELRTNDRRRAGG
                     DAVFLNSRGGRISRQACWTIVRRAGERVGLHDRLSPHVLRHSCATHMLDHGADLRVVQ
                     ELLGHATISTTQVYTKVSPERLRAVYDRAHPRARSRHG"
     gene            3193..>3504
                     /locus_tag="VFX21_07740"
     CDS             3193..>3504
                     /locus_tag="VFX21_07740"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX5095887.1"
                     /translation="MSEPTHAALREQLAAERERVTGQLVELGVDRSSFDEGFADSGQV
                     TAERGEVEALVGTLRETLLDINDALEKFDAGTYGTCEDCGNQIAEARLEAMPMARFCI
                     TC"
BASE COUNT          593 a         1191 c         1212 g          508 t
ORIGIN      
        1 acgttgccag cgtgatgccg atgccggcga gcaccggcgc aatgcgcggt gagtaacgcg
       61 tctggaatcc gaggaggtca ccggcgcgca acaacacgaa gtgcagcggc cccatcgcag
      121 agaacagatc cttgtagggc cactgcccgt gacgcatccc gatgatcgac gcgccgtaca
      181 cactctcgtc gaagccgacg tggcgcgtcg cgaagaagat gggcaaccgc acgatcgccg
      241 cacccgcggc cagcgcgacc ggcaccgtcc acgtgcgccg gcgcgtcggg gtgacgggct
      301 cggccgctgc ggtcatggga aaacgctagg tgacgacggg atgatcgttt gggtcactga
      361 tgggggtact tgcgggtggt accgtcgccg ggcgaacgca gcgatgcctg gatgcctgcg
      421 ggcgcaacct cgaaaggaat gccggtggcc aagcacgtct tcgtgacggg tggagtggcg
      481 tcgagcctcg gcaaggggct caccgcgtcg tcgctgggtc gactcctcaa gacccgcggg
      541 ctgcgggtca cgatgcagaa gctcgacccc tacatcaacg tcgaccccgg cacgatgaac
      601 cccttccagc acggcgaggt gttcgtcacc gacgacaagg gcgagaccga cctcgacctc
      661 ggccactacg agcgcttcgt cgacgtcgcg ctcacccgca agtcgaacgc gacgacgggt
      721 tccatctacc aggcggtgct ggccaaggag cgcaagggcg agtacctcgg tgagacggtg
      781 caggtcatcc cgcacatcac caacgagatc aagtcgcgca tcaacgcgct cgccaccgaa
      841 gacgtcgacg tcgtcatcac cgagatcggc gggacggtcg gcgacatcga gatcctcccg
      901 ttcctcgagg cgatccggca gttccgcaac gaggtcggcc gcgagaacgt cttctacgtg
      961 cacgtcacgc tgatgcccta catcggcccc gcgggcgagc agaagaccaa gccgacgcag
     1021 cactcggtca ccgagctgcg cgcccgcggt atccagcccg acgccatcgt gtgccgcagc
     1081 gaccggccga tcccgacccg gctcaaggag aagatctcgg ccctgtgcga cgtgcccgtc
     1141 gagggtgtcg tgtccgcgat cgacgccgac atcctctacg agatcccgct cgtgctgcac
     1201 gacgaagggc tcgacagcta tctctgccgc gtgctgcacc tcgacgaccg cgaacccgac
     1261 ctcaccgaat ggcgcgcact cgtcgaccgc gtgcgcgccg ccgaccaaga ggtgcacatc
     1321 gggctcgtcg gcaagtacat caacttgccg gacgcgtacc tctcggtcgt cgaggcgttg
     1381 aagcacggtg gttacgcgtc gggcgcgaag gtcgtcgtcg actggatcgc ggccgacgac
     1441 accgaaggcc tgctcgcgga agggcggctc gcggggctcg acggcatcgt cgtgccgggc
     1501 gggttcggcg tgcgcggcat cgagggcaag atcgcggcgg ccggctacgc gcgcgagcac
     1561 ggcatcccgt acctcggtct gtgcctcggt ctgcacgcgg ccacgatcga gttcgcccgc
     1621 aacgtgtgcg ggctcgcagg cgcgaactcg tcggagttcg actcgcagtc gccgcacccg
     1681 gtcatcgacc tgctcgacga gcagcgcgac gtcgtcgaca tgggcggcac catgcgcctc
     1741 ggcgcgtacc cggccaagct cgaggcgggc acgatcgtgc gcaaggtcta cggcgacgaa
     1801 gtcgtctacg agcgccaccg ccaccgctac gagctgaaca accggtaccg ccaggtgttc
     1861 gaggaaaacg gcatgcgact gtcggggacg agccccgacg gcttgctcgt cgagttcatc
     1921 gagttggtgg accacccgtt cttcgtcgcg acgcaggcgc accccgagtt caagagccgg
     1981 cccaaccgcc cgcacccact cttcgcgggc ttcgtcgaag ccgctcgcga acgggccgag
     2041 ggtcgtgccc cgcggctgcc gatcgacgag atcgaaaccg tcacgctgta atgcccggtg
     2101 agtggccgtg cggacgcagc ggaggccttc ccgcggcgaa gccgcccctc ggcgcgtagc
     2161 gagggagcca tagtcatgga cgagctcgac aagctgctcg cagaacacta cgagtggctc
     2221 tcggtcgagc gcgggctcgc gaagaactcg ttggccgcct accgccgcga cctgaatcgg
     2281 tacacggaat tcttgcgggc gcgcaacatc acggatccgg ccgaggtcgg tgagcacacg
     2341 gtgcacgagt tcgtcgagta cctgcaggcg ctgaccgacg acgacggacg cccgttgctc
     2401 gcgccgtcgt cgcgagcgcg cgcgatcgtc tcggtgcggt cgttccaccg gttctgcgcg
     2461 gtcgaggggt tcctgccgag tgacccgacc gaggatgtcg gcgcgccgcg tgtgccgcag
     2521 ggcatcccca aggcgctcga cgaggaggag gtgaccgcgt tgctcggtgc ggtcaccggt
     2581 gattccgcgt tggcgcaacg cgaccgcgcc ctgctcgaga cgctgtacgc gaccgggatc
     2641 cgtatcagcg aggcggtcgg gctcgaccag ggcgacgtcg atctcgaaga cgggctgttg
     2701 cgcgtgctcg gcaagggcga caaggaacgc atcgtgccgg tcgggcgttc ggcgcggcgc
     2761 gcgttgactg cgtacttcgg cgacggcttc ctcgagctgc gcaccaacga ccgccggcgg
     2821 gccggaggtg acgcggtgtt cctgaactcg cgtggtgggc gcatctcgcg ccaggcgtgc
     2881 tggacgatcg tgcggcgcgc gggggagagg gtcgggttgc acgatcgcct gtcgccgcac
     2941 gtgttgcgac actcgtgcgc gacgcacatg ctcgaccacg gtgccgacct gcgcgtcgtg
     3001 caggagctcc tcggtcacgc gacgatctcg acgacacagg tgtacacgaa ggtctcgccc
     3061 gagcggctgc gagccgtcta cgaccgggcg caccccagag cgcgatcccg ccacggatag
     3121 tcgtgagaaa cccgttcggt tccggcggag cgcccgagcc gcccgaccag gggcggaggt
     3181 acacttgccg ccatgtccga gccgacgcat gcagccctgc gcgagcaact cgcggccgag
     3241 cgcgagcggg tcaccggcca gctcgtcgag ctcggcgtcg accggtccag cttcgacgaa
     3301 ggcttcgccg actccggtca ggtgacagcc gagcggggcg aagtcgaggc cctcgtcggc
     3361 acgctgcgcg agacgctgct cgacatcaac gacgcgctcg agaagttcga cgcgggcacc
     3421 tacggcacgt gcgaggactg cggcaatcag atcgccgagg cccggctcga agcgatgccg
     3481 atggcgcggt tctgcattac ctgc
//
LOCUS       DASUGA010000392         2314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_996950, whole genome shotgun sequence.
ACCESSION   DASUGA010000392 DASUGA010000000
VERSION     DASUGA010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996950"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1200
                     /gene="gap"
                     /locus_tag="VFX21_07745"
     CDS             154..1200
                     /gene="gap"
                     /locus_tag="VFX21_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX5095888.1"
                     /translation="MAVRVAINGFGRIGRLVFRALVEQGLLGTRLEVVAVGDIVPADN
                     LAYLLKYDSTQGRFAGTVTSKKSSPDKAEDDVLVVNGKEILVTSAKTPAELPWQKLGV
                     QVVIESTGLFTDAEKAKGHLTAGAKKVIITAPAKGEDITVVLGVNDEKIDYQKHNIIS
                     NASCTTNCLAPLVHVLLKEGFGLTEGLMTTIHAYTATQKTVDGPSKKDWKGGRTAAQN
                     IIPSSTGAAKAVGLVLPEIKGKLTGMSFRVPTPTVSVVDLTFKTQKDTSLEEIKKAMK
                     RASETYMKGILEYTEDEVVSSDFIHSRASSVFDAGSSIELNKNFFKLVSWYDNEWGYS
                     NRVVELTSKVAAKL"
     gene            1315..1467
                     /locus_tag="VFX21_07750"
     CDS             1315..1467
                     /locus_tag="VFX21_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095889.1"
                     /translation="MTQAVWELPIPEVAVLVGVHFYNQALVLDPGANPFGAVVSDAAE
                     GVVGHW"
     gene            1700..>2314
                     /locus_tag="VFX21_07755"
     CDS             1700..>2314
                     /locus_tag="VFX21_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX5095890.1"
                     /translation="MAKNLTDLDLSGKRVLVRVDFNVPLDGKGQITSDARIRESLPTI
                     KAILKAGGRPVLMSHLGRPKGKIVESMRLQPVAQRLGELLGSRVDYVKDCIGPEAEAA
                     SKALPAGACLVLENLRFRDAEEAGDEAFAQALARNGDVYVNDAFGTAHRAHASVSVIC
                     KHLPAAAGLLMQKELAAFGKVLTKPDRPFVAILGGAKVSDKLPVI"
BASE COUNT          416 a          760 c          771 g          367 t
ORIGIN      
        1 ccgcgcgatc cgcgacgttg ctctcgaagc ggcagaggca cctcgagcac aggtccagca
       61 ggcgcgcgaa actgtccatt tggctcggaa gtgaatttcg gtagactgct cggccgtttt
      121 tcgccgcaac aaccagcagg agcttacctc gtcatggcag tccgggtcgc tatcaacggt
      181 ttcggtcgca tcggtcgcct cgtcttccgc gccctcgtcg aacaggggct gctcggcacc
      241 agactcgagg tcgtcgcggt cggcgacatc gtgccggccg acaacctcgc ctacctgctc
      301 aagtacgact ccacgcaggg gcgctttgcc ggcacggtca cctcgaagaa gtcctcaccc
      361 gacaaggccg aggacgacgt gctcgtcgtg aacggcaagg agatcctggt cacgagcgcg
      421 aagacgcccg cggagctgcc gtggcagaag ctcggcgtgc aggtcgtgat cgagtcgacc
      481 ggtctgttca ccgacgccga gaaggccaag ggccatctca ccgccggcgc gaagaaggtc
      541 atcatcaccg cacccgcgaa gggcgaggac atcacggtcg tgctcggcgt caacgacgag
      601 aagatcgact accagaagca caacatcatc tcgaacgcgt cgtgcacgac gaactgtctc
      661 gcgccgctcg tgcacgtgct gctgaaggag ggcttcggtc tcaccgaggg gttgatgacg
      721 acgatccacg cctacaccgc gacgcagaag accgtcgacg gcccgtcgaa gaaggactgg
      781 aagggcggcc gcaccgcggc gcagaacatc atcccgtcga gcacgggtgc ggcgaaggcg
      841 gtcggtctcg tgctgcccga gatcaagggc aagctgacgg gcatgtcgtt ccgcgtgccg
      901 acgccgaccg tgtccgtcgt cgacctcacg ttcaagacgc agaaggacac gtcgctcgag
      961 gagatcaaga aggcgatgaa gcgcgcctcc gagacctaca tgaagggcat cctcgagtac
     1021 accgaggacg aagtcgtctc gtcggacttc atccacagca gggcttcgtc ggtgttcgac
     1081 gcgggctcgt cgatcgagct gaacaagaac ttcttcaagc tcgtgagctg gtatgacaac
     1141 gagtggggct actcgaatcg cgtcgtcgag ctgacctcca aggtcgccgc gaagctgtga
     1201 aacctcgggg ccggcccccc tcaatgttcc ctgtttgttg tagtccacgg cctgcaggac
     1261 gggggcgacc agctggccca gctgcggagg acttcgcatc gaggggctca ggctgtgacc
     1321 caggcggtct gggagctgcc gattcccgag gtcgcggttc tggtcggcgt gcacttctac
     1381 aaccaggcgt tggtgctcga tccgggtgcc aaccccttcg gcgccgtcgt gtcggatgcg
     1441 gccgagggag tcgtcggcca ctggtgagac ggggcgcagc ccccaagtcc gctcggtgcc
     1501 acggtgctcg ttgtcgcgcc gtcgagaagg ccacgtcgca ttttcgcgcg aacaagaccg
     1561 cttcgcggta gccgacgctc gtgacggccc gggactgttg gcccgtgcgc cgcatcagca
     1621 agatcccgct gggcggcgaa ccgcaccggc gaacgtggca ccttccccgt ggtcgcggca
     1681 agatcacggc ttcgctgcca tggccaagaa cctcaccgac ctcgacctgt ccggcaaacg
     1741 cgtgctcgtg cgcgtcgact tcaacgtgcc cctcgatggc aagggccaga tcacgagcga
     1801 tgcccgcatc cgcgagagcc tgccgacgat caaggccatc ctgaaagcag gagggcgtcc
     1861 ggtgctgatg tcgcacctcg ggcggcccaa gggcaagatc gtcgagtcga tgcggctgca
     1921 accggtcgcg cagcggctcg gcgagctgct cggttcgcgc gtcgactacg tgaaggactg
     1981 catcggcccc gaagccgagg ccgcgtcgaa ggcgctgccg gccggcgcgt gtctcgtgct
     2041 cgagaacctg cgcttccgcg acgccgagga ggcgggcgac gaggcgttcg cgcaggcgct
     2101 cgcgcggaac ggcgacgtct acgtgaacga cgcgttcggc accgcgcacc gcgcgcacgc
     2161 ttccgtcagc gtgatctgca agcacctgcc cgcggcggcc gggttgttga tgcagaagga
     2221 gctcgccgcg ttcggcaagg tgctgaccaa accggaccgg ccgttcgtcg cgatcctcgg
     2281 cggcgcgaag gtcagcgaca agctgcccgt gatc
//
LOCUS       DASUGA010000393         9372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_988406, whole genome shotgun sequence.
ACCESSION   DASUGA010000393 DASUGA010000000
VERSION     DASUGA010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 9372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9372
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988406"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VFX21_07760"
     CDS             <1..186
                     /locus_tag="VFX21_07760"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX5095891.1"
                     /translation="LTANEMFDAIAALPYRQRTAIVLRYWHDADDRAIADALGCRIGT
                     VGSLVHRAHAVLRRGLP"
     gene            183..1508
                     /locus_tag="VFX21_07765"
     CDS             183..1508
                     /locus_tag="VFX21_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095892.1"
                     /translation="MSEFDDRVRRSLQQQGELIHDAPAPAELTDRIVRKERRARRGLT
                     VALVVALLAGPTLGFVAGRGRPEETKKVAKTATAKTDTGVVIHDDKVPKIPGDAALSA
                     GATQDSFAAAGSRTAESQGIAIAAGTWTGPYGVEMARFFDRDAGNAKVRVYHAEIETP
                     VSAGPPWWQPADYCFPNGVVQADVSTDDIVGIVSGSTYASYKDTSMGGSLGIAGVAEQ
                     APHWIAIAQSTADVATIRVTFPGGGTDEMKPVDGVAVLVAPAAVNDDQLVNDVEGLKA
                     HAQSLDRSGAVVAEADLSLYGGYLGMSDPGCSAPQELPAPGAEQPADPEAARAEIASH
                     FGGAGGASKVSDILGNLDDPRGMEETWNELLNGPFAEQAKKAETQFRDLVFLSATRAA
                     VQYDVHIPDYMDFDNRFAEYVLIDGQWKETRESLCRDIALAGVTCPPPE"
     gene            complement(1513..3453)
                     /locus_tag="VFX21_07770"
     CDS             complement(1513..3453)
                     /locus_tag="VFX21_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006517169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5095893.1"
                     /translation="MSTTWHKVATLDELPDGRVKTVVAGRKSLALTHCNGRYGALDNH
                     CPHQGGPLGEGSIENGWLRCPWHGYDYSPATGTPPPGFSDAPACFPVEVRADGVYVEL
                     PDEVPRARTVSDVMVETMCAWGVDHVFGMVGHSNLGFADAMRGAEARGDLQFIGIRHE
                     GAAAFAASAYGKLTGRLAACFAIAGPGSTNLLTGLYDAKVDRAPVLAISGQVPSNVRG
                     RGAFQDLDLNAAFADVAVYSQTVQASSDHAELMNLACKHALLQRGVAHLVLPDEVQVL
                     AAAPGADAGGPSGRLPAQDIAPPEAAVEDAIQLLGRAKRPVFVVGHGARFDMDDVMAL
                     AERLHAPVLTTFKAKGIVADDHPLGCGVLGRSGTPIASWFMNESDLIVVFGASFSNHT
                     GIAPYKPIVQVDFDADALGRFHPVTVPVLGHVGVTARRLLQGCDAAKIETIDQRDEVA
                     TRRAIWSAEKERRMHDDHGRGVNSASIFAAMQHHVPDDAVLAVDVGNNTYSFGRYFEC
                     RRQSVLMSGYLGSIGFGFPAAMGAWAAAPDRTIVAVTGDGGFGQYLAEITTAVKYGMN
                     ITHVLLNNGQLGKITKEQRAAEWDVWQTGLHNPDFAAYAQLCGAFGVRVTDRAQLDDA
                     LAAALAHDGPALVDIVTDADLV"
     gene            complement(3450..5057)
                     /locus_tag="VFX21_07775"
     CDS             complement(3450..5057)
                     /locus_tag="VFX21_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011456242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEX5095894.1"
                     /translation="MNLVRIATWSEIPEREPVGAMVEGVDLVVIRRGDEHSVLYGRCL
                     HRGARLADGHVVGDDLLCGLHGWDYRIDSGVSAYNNSEVLEKFTSFVEDDGLFVDRDE
                     VIRWILRHPQPYDPDVYQGLYTDPHMVIEEPYVMRIHEYAANGLSKVGHHGPVAAMGV
                     PRQELPSWDNIQFVTAQLARLPLLDDEPVGTEICIGPNAARPLWLDIPLFVSDMSFGA
                     LSQEAKTALARGAQLAGTGICSGEGGMLPEEQSENSRYFYELASARFGWSWDVLPKVQ
                     AFHFKLGQGAKTGTGGHLPGHKVSGRIAEVRQLPEGTAAISPSRFPDWHTVEDFRRFA
                     DEVRDRSGGIPIGVKLSAQHVEDDLDAALSIGVDYVIIDGRGGGTGAAPLLFRDNISV
                     PTIPALARARRHLDRRGRRDVTLVATGGLRVAADFAKALALGADAVAIANSAIQAIGC
                     LGMRACHTNNCPVGIATQKPHLRARLPVDEAAQRLDRFLGATVELMQVLARACGHDHL
                     SKFVLDDLTTFDRDMAALSGVEYGGVR"
     gene            5206..6543
                     /locus_tag="VFX21_07780"
     CDS             5206..6543
                     /locus_tag="VFX21_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEX5095895.1"
                     /translation="MTATANPFLMGNYAPVADECTADQLDVVGQIPAELRGRYLRIGP
                     NPYAMPDGPYHWFMGDGMVHGVELADGRANWYRNRWVRTDGIADAMGEPHVGGPTPPM
                     YDSSNTNVLGHAGRILSLTEGAIPFELSPTLDTKRRQDFGGPLPGGLTAHPKVDPVTG
                     ELHAFTYWFAPPFCYYHVVDAQGNLVRTEPITLPRSVMMHDFALTRDNVIFFDLPVVF
                     DLEVMPFPFRWDDDHQARIGVMPRTGGDADVTWIDIDPCYVFHPMNAYEDGGTIVVDV
                     PRHPTMFASQGELIEGPNRGGPPALHRWTIDVGAGKVRDEVVDERGQEFPRVNESLLM
                     SKHRYGYAVQVDSADAFEGSTYLKHDFERGAVEEHSFGVGAGPGEFVFVPAESGTSED
                     DGWLMGFVYDAATDTSSFQIVDAHDFAAAPVAVVKLPRRVPYGFHGNWVPDAR"
     gene            complement(6547..8010)
                     /locus_tag="VFX21_07785"
     CDS             complement(6547..8010)
                     /locus_tag="VFX21_07785"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX5095896.1"
                     /translation="MRRRAAVVLGLGATVAAVAAVLAVRARRRREHATAVAARSTRAR
                     TLTLATVGARSGAAHVVHRARRAVTPAAERAALDEAHQIRTTAQVVEALGNMKGALMK
                     LGQMASYLDDGMPEPMRDALASLQQDAAPMAPELCAQVIRSELGRDPEHLFAAWDPDP
                     IAAASIGQVHRARTRAGLEIAVKVQYPGVDDAIRSDLMASDLLFRALSLLFPGLDPGP
                     LVEELRARLVEELDYTREAENQRLFTGYYRGHPFIRVPRVVDELSTARVLTTEYANGA
                     RFAEVEEWSQPERDLAAEAIYRFVFRSLYRLHAFNGDPHPGNYLFHGNGIVTFLDFGL
                     VKYYEPTDVALFEDLIRAMVLEHDITRFRSVLEEHDILRRDPSMTDDDVREYFGHFYE
                     FVMDDKVVTFSPEYASETVRRFFDVSGPHAHVAKKSNVPPAFVITQRINLGLHAVLGR
                     LRATANFRKIAEELWPIVDGPPTTPMGQAEADWLASK"
     gene            8084..9280
                     /locus_tag="VFX21_07790"
     CDS             8084..9280
                     /locus_tag="VFX21_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5095897.1"
                     /translation="MPTDVTTDEQRVVELCDQLLAEHDPKTTPANVFLGAQYDLGLAW
                     VHFPEGSGGLGLSPKLQQVINQKVVRAGGPIAYARNPIGAGMCAPTVVVHGSDEQKQR
                     YLRPLFAGEEIWCQMFSEPGAGSDVAGLSTRAERDGDEWVLNGQKVWTTLAHVSRFGI
                     VLARTNPEAVKHKGMTMFVIDMHAPGVEVRPLVQATGEAEFNEVYMTDVRIPDSERLG
                     DVGEGWHVSLTTLMNERVSIGGQVQPRGSGIIAIAMKTYENSNQRGNPIAREKLAQFW
                     IESEVNRLTNMRASQLRAKGTPGPEGSVGKMAMAGLNQRLMDFVMDLLGPEAMLYPGG
                     YPMDRPTTAMDLSKPQKAFLRVQANSIEGGTTNIMKNILSERVLGLPGEPRADKDVPW
                     SQVPRS"
BASE COUNT         1478 a         3169 c         3325 g         1400 t
ORIGIN      
        1 ctgaccgcga acgagatgtt cgacgcgatc gccgcgttgc cgtaccggca gcgcaccgcg
       61 atcgtgctgc ggtattggca cgacgcggac gaccgcgcga tcgcggacgc gctcggctgc
      121 cggatcggga cggtggggtc actcgttcac cgcgcgcacg cggtactacg aaggggattg
      181 ccatgagtga gttcgacgac cgcgtgcggc gcagcctgca acagcaaggc gagttgatcc
      241 acgacgcgcc ggcgcccgcg gagttgacgg atcgcatcgt gcgcaaggaa cggcgggccc
      301 ggcgcggcct cacggtcgcg ctcgtcgtcg cgctgctggc ggggccgacg ctcggcttcg
      361 tggcgggtcg agggcggccg gaagagacca agaaggtcgc caagaccgcg accgccaaga
      421 cggacaccgg tgtcgtcatc cacgacgaca aggtgccgaa gatccccggc gacgcggccc
      481 tgagcgcggg cgcgacgcag gattccttcg ccgccgcggg ttcgaggacg gccgagagcc
      541 aagggatcgc catcgccgcc ggcacgtgga ccggtcccta cggcgtcgag atggcgcggt
      601 tcttcgaccg cgacgcgggg aacgcgaagg tgcgcgtgta ccacgccgag atcgagacac
      661 ccgtgtccgc gggaccgccc tggtggcagc ccgccgacta ctgcttcccg aacggcgtcg
      721 tgcaagccga cgtctcgacc gacgacatcg tcggcatcgt gtcggggtcg acctacgcgt
      781 cgtacaagga caccagcatg ggcggttcgc tgggaatcgc gggcgtggcc gagcaggcgc
      841 cgcactggat cgcgatcgcg cagtcgaccg cggacgtcgc gacgattcgc gtgacgttcc
      901 ccggcggcgg caccgacgag atgaagccgg tcgacggtgt tgcggtgctc gtcgcgcccg
      961 cagccgtcaa tgacgaccag ctcgtgaacg acgtcgaggg cctgaaggcg cacgcgcagt
     1021 cgctcgacag atcgggcgcg gtcgtcgccg aagccgacct cagcctgtac ggcggctacc
     1081 tcggtatgag tgacccgggc tgcagcgcgc cgcaggaact gccggctccc ggggcggagc
     1141 aacccgccga cccggaggcc gcgcgcgccg agatcgcgtc gcacttcggt ggcgcgggcg
     1201 gggcatcgaa ggtcagcgac atcctcggga acctcgacga tcctcgcggg atggaagaga
     1261 cgtggaacga gctcctcaac ggtccgttcg cggagcaggc caagaaggcg gagacacagt
     1321 tccgtgatct cgtgttcctc tccgcgacgc gtgcggccgt ccagtacgac gtccacatcc
     1381 ccgactacat ggacttcgac aaccggttcg cggagtacgt gttgatcgac gggcagtgga
     1441 aggagacgcg cgagtcgttg tgccgtgaca tcgcgctcgc gggtgtcacg tgcccgccgc
     1501 cggagtaacg cgctagacga gatcggcgtc ggtgacgatg tcgacgagtg cggggccgtc
     1561 gtgcgcgagc gcggcggcca gcgcgtcgtc gagctgggcc cggtcggtga cccgcacacc
     1621 gaacgcgccg cacaactgcg cgtaggccgc gaagtcgggg ttgtgcaggc cggtctgcca
     1681 gacgtcccac tctgcggcgc gttgctcctt ggtgatcttg ccgagctggc cgttgttcaa
     1741 cagcacgtgc gtgatgttca tcccgtactt caccgcggtc gtgatctcgg cgaggtactg
     1801 accgaagccg ccgtcgccgg tcacggcgac gatcgtgcgg tccggcgccg ccgcccacgc
     1861 acccatggcg gcggggaagc cgaacccgat cgagccgagg taccccgaca tgagcaccga
     1921 ctggcggcgg cactcgaagt agcgcccgaa cgagtacgtg ttgttgccga cgtcgaccgc
     1981 gagcactgcg tcgtctggga cgtggtgttg catcgcggca aagatcgacg cgctgttcac
     2041 gccccttcca tggtcgtcgt gcatccgccg ttccttctcg gcggaccaga tcgcgcgccg
     2101 cgtcgcgact tcgtcgcgtt ggtcgatcgt ctcgatcttc gccgcgtcgc agccctgcag
     2161 caggcggcgc gcggtgacgc cgacgtggcc gaggacggga acggtaacgg ggtggaagcg
     2221 accgagcgcg tcggcgtcga agtcgacctg gacgatgggc ttgtacggcg cgatgccggt
     2281 gtggttggag aacgacgcgc cgaacacgac gatcaggtcc gactcgttca tgaaccacga
     2341 cgcgatcggt gtgccgctgc ggccgagcac gccgcatccc agcgggtggt cgtcggcgac
     2401 gatgcccttg gccttgaacg tcgtgagcac cggcgcgtgc aggcgttcgg cgagcgccat
     2461 cacgtcgtcc atgtcgaacc gcgcgccgtg cccgacgacg aacaccggcc gtttggcccg
     2521 cccgagcaac tggattgcat cctcgaccgc tgcctccggc ggcgcgatgt cctgcgccgg
     2581 cagccgcccg ctcgggccgc ccgcgtctgc gccgggcgcg gccgcgagca cctgcacttc
     2641 gtcgggcagc acgaggtgcg cgacgccgcg ctggagcaac gcatgcttgc acgcgaggtt
     2701 catcagctcc gcgtgatcgg acgacgcctg cacggtctgc gagtacaccg cgacgtcggc
     2761 gaacgcggca ttgaggtcga ggtcctggaa cgcaccgcgc ccacgcacgt tcgacggcac
     2821 ctgacccgag atcgcgagca ccggcgcgcg gtcgaccttg gcgtcgtaga gaccggtcag
     2881 gaggttggtc gaacccgggc ccgcgatcgc gaagcacgcc gcgagccggc cggtgagctt
     2941 cccgtaggcc gacgctgcga acgcggccgc gccttcgtga cggataccga tgaactggag
     3001 atcgccgcgt gcttcggcgc cgcgcatggc gtccgcgaac ccgagattcg agtgcccgac
     3061 catgccgaac acgtggtcga cgccccatgc gcacatcgtc tcgaccatca cgtcggaaac
     3121 ggtgcgggcg cgcggcactt cgtcggggag ctcgacgtag acgccgtcgg cgcgcacctc
     3181 gaccgggaag cacgcgggcg cgtccgagaa gccgggcggc ggcgtcccgg tcgccggcga
     3241 gtagtcgtag ccgtgccacg ggcagcgcaa ccagccgttc tcgatcgatc cttcgccgag
     3301 cggaccgccc tggtgcgggc agtggttgtc gagcgcgccg tagcgcccgt tgcaatgtgt
     3361 gagcgcgagg ctcttgcgcc ccgcgacgac cgtcttcacg cggccgtcgg gcagctcgtc
     3421 gagagtggcg accttgtgcc aggtcgtgct catcgcacgc cgccgtactc gacgcccgac
     3481 agtgccgcca tgtcgcggtc gaacgtcgtg aggtcgtcga gcacgaactt cgagagatga
     3541 tcgtgcccgc acgcgcgggc gaggacttgc atcaactcga cggtggcgcc caggaaccga
     3601 tcgagccgct gcgcggcctc gtcgaccggc aggcgggcgc gcaagtgcgg tttctgcgtc
     3661 gcgatgccga cggggcagtt gttggtgtgg cacgcgcgca tcccgaggca gccgatcgcc
     3721 tggatcgcgg agttcgcgat tgcgaccgcg tcggcaccga gcgccaacgc cttggcgaag
     3781 tcggccgcca cccgcaggcc gcccgtcgcg acgagcgtga cgtcgcgccg gccgcgccgg
     3841 tcgagatggc gccgggcgcg cgccagcgcg gggatcgtcg ggaccgagat gttgtcgcgg
     3901 aacagcaacg gcgccgcgcc cgtgccgccg ccgcgtccgt cgatgatcac gtagtcgacg
     3961 ccgatcgaca gtgccgcgtc gaggtcgtcc tcgacgtgct gcgcggacag cttcacgccg
     4021 atcgggatgc cgcccgagcg gtcgcgtacc tcgtcggcga aacggcggaa gtcctcgacg
     4081 gtgtgccagt cggggaagcg cgacggggag atcgcggcgg tcccttccgg cagttgccga
     4141 acttcggcga tcctgcctga caccttgtgg cccgggagat ggccgccggt cccggtcttt
     4201 gcgccctgcc cgagcttgaa gtggaacgcc tgcaccttcg ggagcacgtc ccacgaccat
     4261 ccgaagcgcg ccgacgcgag ctcgtagaag tagcgcgagt tctcagactg ctcctcgggc
     4321 agcatgccgc cctcacccga gcagatgccg gtacccgcga gctgcgcgcc gcgcgcgagc
     4381 gcggtcttcg cctcctgcga cagcgcgccg aagctcatgt cggagacgaa caacgggatg
     4441 tcgagccaca acggccgggc ggcgttcggg ccgatgcaga tctcggtgcc gaccggctcg
     4501 tcgtcgagca gcggcaggcg cgcgagctgc gcggtgacga actggatgtt gtcccacgac
     4561 gggagctctt ggcgcggcac gcccatcgcc gcgaccggtc cgtggtgccc gaccttgctg
     4621 agaccgttcg cggcgtactc gtggatccgc atcacgtacg gctcttcgat caccatgtgc
     4681 gggtcggtgt agagcccttg gtacacgtcg gggtcgtacg gctgcgggtg gcgcaggatc
     4741 caccggatga cttcgtcgcg gtcgacgaac agcccgtcgt cttccacgaa cgacgtgaac
     4801 ttctcgagca cttcactgtt gttgtacgcg ctcacgccgg agtcgatgcg gtagtcccag
     4861 ccgtgcagac cgcacagcag gtcgtcaccg acgacgtgcc cgtcggcgag ccgcgcgccg
     4921 cgatgcaggc agcggccgta cagcacggag tgctcgtcgc cgcgccggat gacgacgagg
     4981 tcgacgcctt cgaccatcgc gccgaccggc tcgcgctcgg ggatctcgct ccaggttgcg
     5041 atgcggacga ggttcacgat gcgtgttgta ctcccggcgt ggggccgccg gaagtacttc
     5101 gcaggcatcg gcgcgtgtgg atcagtgcgc gacgaggggc gttgacggct gattataacg
     5161 ctgttatggt cgctgggacc ccggcgcccg gcgagaggac gcgtgatgac cgccaccgcc
     5221 aacccgttcc tgatgggcaa ctacgccccg gtggccgacg agtgcaccgc cgaccagctc
     5281 gacgtcgtcg ggcagatccc cgccgagctg cgcggccggt acctgcgcat cggtcccaac
     5341 ccgtacgcaa tgcccgatgg tccgtaccac tggttcatgg gcgacggcat ggtgcacggc
     5401 gtggagctcg ccgacgggcg cgcgaactgg taccgcaacc ggtgggtgcg caccgacggg
     5461 atcgcggacg cgatgggcga gccccacgtc ggagggccga cgccgccgat gtacgactcg
     5521 agcaacacga acgtgctcgg tcacgcgggc cgcatcctgt cgctcaccga gggcgccatc
     5581 ccgttcgagc tgtcaccgac gctcgacacg aagcggcgcc aggacttcgg cggtcccttg
     5641 cccggtgggc tgactgcgca cccgaaggtc gacccggtga ccggagagct gcacgcgttc
     5701 acgtattggt tcgcgccgcc gttctgctac taccacgtcg tcgacgcgca ggggaacctc
     5761 gtgcgcactg aaccgatcac gctgccccgg tcggtgatga tgcacgactt cgcgttgacg
     5821 cgcgacaacg tgatcttctt cgacctgccg gtcgtgttcg acctcgaggt catgccgttc
     5881 ccgttccgct gggacgacga ccaccaggcg cgcatcggcg tcatgccgcg caccggcggc
     5941 gacgccgacg tcacgtggat cgacatcgac ccctgctacg tgttccaccc gatgaacgcg
     6001 tacgaggacg gcggaacgat cgtcgtcgac gtcccgcgcc acccgacgat gttcgcgtcg
     6061 cagggcgagc tgatcgaagg cccgaaccgt ggcggcccgc cggcgttgca ccggtggacg
     6121 atcgacgtcg gcgcgggcaa ggtgcgcgac gaggtcgtcg acgagcgcgg ccaggagttc
     6181 ccgcgcgtca acgagtcact gctgatgtcg aagcaccgct acggctacgc ggtgcaggtc
     6241 gacagtgccg acgcgttcga aggcagcacg tacctgaagc acgacttcga acggggcgcg
     6301 gtcgaggagc actcgttcgg cgtgggggcc ggtcccggcg agttcgtgtt cgtgccggcc
     6361 gagagcggca cgagcgagga cgacggctgg ctgatgggct tcgtctacga cgccgcgacc
     6421 gacacgagca gcttccagat cgtcgacgcg cacgacttcg ccgccgcgcc cgtcgcggtc
     6481 gtgaagctgc cgcgccgagt gccgtacggc ttccacggca actgggtccc cgacgcgcgc
     6541 tgacggttac ttcgacgcga gccagtcggc ttcggcctga cccatcggtg tcgtcggcgg
     6601 gccgtcgacg atcggccaca gctcttctgc gatcttgcgg aagttcgcgg tcgcccgcag
     6661 acggccgagc accgcgtgca gcccgaggtt gatgcgttgc gtgatgacga acgcgggcgg
     6721 cacgttcgac ttcttcgcga cgtgcgcgtg cggaccggac acgtcgaaga agcgccgcac
     6781 cgtctcggac gcgtactccg ggctgaacgt gacgaccttg tcgtccatca cgaactcgta
     6841 gaagtgcccg aagtactcgc ggacgtcgtc gtcggtcatc gacggatcgc ggcggaggat
     6901 gtcgtgctcc tcgagcacgg agcggaagcg cgtgatgtcg tgctcgagca ccatggcgcg
     6961 gatcaagtct tcgaacagcg cgacgtcggt gggctcgtag tacttcacca atccgaagtc
     7021 gaggaacgtg acgatgccgt tgccgtggaa cagatagttc ccggggtgcg ggtcgccgtt
     7081 gaacgcgtgc agccggtaga ggctgcggaa cacgaaccgg tagatggcct ccgccgcgag
     7141 gtcgcgctcg ggctgtgacc actcctcgac ctcggcgaag cgggcgccgt tggcgtattc
     7201 ggtcgtgagc acgcgcgcgg tcgacagctc gtcgacgacg cgcggcacgc ggatgaacgg
     7261 atggcccctg tagtagccgg tgaacagtcg ctgattctcg gcctcgcgcg tgtagtcgag
     7321 ctcctcgacg aggcgggccc gcaactcttc gacgagcgga ccgggatcga gacccgggaa
     7381 caagagcgac agcgcgcgga acaagagatc gctcgccatg agatcggagc ggatggcgtc
     7441 gtcgacgccc ggatactgca ccttcaccgc gatctcgagg cccgcgcgcg tgcgcgcacg
     7501 gtgcacctgt ccgatcgacg cggccgcgat cggatcggga tcccacgccg cgaacaagtg
     7561 ctcggggtcg cgtcccagct cactgcggat cacctgcgcg cacagctcgg gtgccatcgg
     7621 cgccgcgtcc tgctgcaggc tcgccaacgc gtcgcgcatc ggctcgggca tgccgtcgtc
     7681 gaggtagctc gccatctggc cgagcttcat caacgcgccc ttcatgttgc cgagtgcctc
     7741 cacgacctgc gcggtcgtgc ggatctggtg cgcctcgtcg agcgcggcgc gctcggcggc
     7801 gggcgtgacc gcgcgccggg cacggtgcac gacgtgggcc gcacccgaac gcgcgccgac
     7861 cgtcgccagg gtcagcgtgc gcgcccgcgt gctgcgcgcg gccaccgcgg tcgcgtgctc
     7921 ccgccgccgc cgggcgcgga ccgcgagcac ggcggccacc gcggccaccg tcgctccgag
     7981 acccagcacc accgccgccc tgcgtcgcac gaggagccat cttggcggag cggaggccgg
     8041 ttcgtagact tgaccgatcg gtcaacttcc ggaggatcgc cccgtgccca cagacgtgac
     8101 caccgacgag cagcgagtcg tcgagctgtg tgaccagctc ctcgccgagc acgatccgaa
     8161 gacgacgccg gcgaacgtct tcctcggcgc gcagtacgac ctcggcctcg cgtgggtgca
     8221 cttcccggag ggcagcggcg gcctcggcct gtcgccgaag ctccagcagg tgatcaacca
     8281 gaaggtcgtg cgcgccggtg gcccgatcgc atacgcgcgc aatccgatcg gcgcgggcat
     8341 gtgcgcgccg accgtcgtcg tgcacgggag cgacgagcag aagcagcggt acctgcgccc
     8401 gctgttcgcc ggcgaggaga tctggtgcca gatgttcagc gagcccggcg cgggctccga
     8461 cgtcgcggga ctctcgacgc gcgccgagcg cgacggtgac gagtgggtgc tgaacggcca
     8521 gaaggtgtgg acgacgctcg cgcacgtgtc gcggttcggc atcgtgctcg cccgcaccaa
     8581 ccccgaagcg gtgaagcaca aggggatgac gatgttcgtc atcgacatgc acgcacccgg
     8641 tgtcgaagtg cgaccgcttg tgcaggcaac gggtgaggcg gagttcaacg aggtctacat
     8701 gaccgacgtg cgcatccccg atagcgaacg gctcggtgac gtcggcgaag gctggcacgt
     8761 gtcactgacg acgctgatga acgagcgggt gtcgatcggc gggcaggtgc agccgcgcgg
     8821 cagcgggatc atcgcgatcg cgatgaagac gtacgagaac agcaaccagc gcggcaatcc
     8881 gatcgcgcgc gagaagctcg cgcagttctg gatcgagtcc gaggtgaacc ggctcacgaa
     8941 catgcgcgcg tcgcaactgc gggcgaaggg cacgccgggt ccggagggtt cggtcggcaa
     9001 gatggcgatg gccggcctca accagcggct catggacttc gtcatggacc tgctcggccc
     9061 cgaggcgatg ctctatccgg gcggctatcc gatggaccgg ccgaccacgg ccatggacct
     9121 gtcgaagccg cagaaggcgt tcctgcgcgt gcaggccaac tcgatcgagg gcggcacgac
     9181 caacatcatg aagaacatcc tcagcgagcg ggtcctgggc ctgccgggcg agccccgggc
     9241 cgacaaggac gtgccgtgga gccaggtgcc ccgctcgtga gcgggacgca gcgtccgggc
     9301 agaccccgtc acttctgttg ctcatgttgc acttgcggtt tatgaggtag ttgcgctgca
     9361 ctgtttctcc tg
//
LOCUS       DASUGA010000394         2840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1038410, whole genome shotgun sequence.
ACCESSION   DASUGA010000394 DASUGA010000000
VERSION     DASUGA010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038410"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VFX21_07795"
     CDS             <1..210
                     /locus_tag="VFX21_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095898.1"
                     /translation="MTIAHQSAMQGSAIRQRRPGVRVVVRPAVARRPVLVRLGLLIAG
                     TAVAIGVAMTITIAAVFVLAGALAG"
     gene            complement(226..924)
                     /locus_tag="VFX21_07800"
     CDS             complement(226..924)
                     /locus_tag="VFX21_07800"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HEX5095899.1"
                     /translation="MRKREIVGGALVAVVAGALVIASVVGAEDGAAPATTASTAGSTA
                     AATTTVATTTSTTTTSTSTTTTTTTIAPLTAPAATSPETAAPSDGPAPPPADPREDVA
                     VTRIGTITIPKIGLHHELYEGVSLTVLDVGPGHWPGTPMPGSAGNVVVGGHRVTHSHP
                     FRDLDQLTPGDEILFDMADGSYATYRVTETFIVNPDAMWIVDPTPDNIVTLFACHPKG
                     SAKQRIVVRAALAA"
     gene            979..1578
                     /locus_tag="VFX21_07805"
     CDS             979..1578
                     /locus_tag="VFX21_07805"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5095900.1"
                     /translation="MQRAVEIAARGASGRVRATDATELAAAVAADPDPTVRAVALGAL
                     ARAAPKRVAATAWRVAAGDADAAVRRRAAQTAPAIGRLVPVAMLVALLRDDDAWVAEA
                     AAFALGERDDATAVAPLEDAARGHADALVREAAVAALGARGVGRDAVLDACHDKPAIR
                     RRAVLALAAFDGPEVEAALQAALEDRDWQVRQAAEDLLH"
     gene            complement(1589..2599)
                     /locus_tag="VFX21_07810"
     CDS             complement(1589..2599)
                     /locus_tag="VFX21_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX5095901.1"
                     /translation="MPRDLAIDLGTANTLVYARGRGIILNEPTVIALNERSQEVLAMG
                     HEAWQMIGRTPGYIIAVRPLRGGAITDFEITQRMIRLLFQRAGVSRLYRARVLICVPS
                     AITHVEQRAVLEAARRAGAAQTYLIEQPMAAAIGAGLPIHEPMGNMVVDIGGGTTEVA
                     VISLGGVVALEALRVGSFDIDAAIQTYVRREYGIAIGEPTAEQIKLAIGSAYAVDDEY
                     KAEVRGRDLMSGLPKTILLLPEEVRAAIEEQVRAICDAVVACLARTPPELAQDLILNG
                     VHLVGGGGMLAGLDKRISEETELPVHLVDAPLECVVLGAGRCLENFDLLKEMFMARSM
                     AR"
     gene            complement(2613..2780)
                     /locus_tag="VFX21_07815"
     CDS             complement(2613..2780)
                     /locus_tag="VFX21_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095902.1"
                     /translation="MEFLLFAVVASVIGIVIVLMRNRRPSSMQHSIDEFERGLRALAP
                     DARNRRPQPPE"
BASE COUNT          387 a          993 c         1023 g          437 t
ORIGIN      
        1 atgaccattg cccaccagag tgccatgcag gggtcggcga tccggcagcg gcgccccggc
       61 gtgcgagtcg tggtgcgccc ggcggtggcc cgcaggccgg tactcgtacg cctcggtctc
      121 ttgatcgccg gcaccgccgt cgcgatcggc gtcgcgatga cgatcacgat cgcggccgtc
      181 ttcgtcctcg ccggcgccct cgccggctga gacgccggcg cgcgctcagg ccgcgagcgc
      241 ggcgcgcacg acgatgcgtt gcttcgcgct gcccttgggg tgacacgcga acagcgtgac
      301 gatgttgtcg ggtgtcgggt cgacgatcca catcgcgtcg gggttgacga tgaacgtctc
      361 ggtgacccgg tacgtggcgt agctgccgtc ggccatgtcg aacaggatct cgtcgcccgg
      421 cgtcagctgg tcgaggtctc ggaacggatg gctgtgagtg acgcggtgcc cgccgacgac
      481 gacattgccg gcactgcccg gcatcggcgt acccggccag tgaccgggac cgacgtcgag
      541 caccgtcagc gacacgcctt cgtacagctc gtggtgcagc ccgatcttcg ggatcgtgat
      601 cgtgccgatc cgggtgaccg cgacgtcttc gcgcggatcg gcgggcggcg gcgcggggcc
      661 gtcgctcggc gccgcggtct cgggcgacgt cgccgcgggc gcggtgaggg gcgcgatcgt
      721 cgtcgtggtc gtggtcgtgc tcgtcgacgt cgtggtcgtc gacgtggtgg ttgccaccgt
      781 cgtcgttgcg gcggcggtcg agcccgcggt cgatgcggtc gtggccggtg ccgccccgtc
      841 ctcggcaccg accacgctcg cgatgacaag cgcgccggcg acaaccgcga caagcgcgcc
      901 acccacgatt tcgcgctttc gcatgaccga ggatcggcag cggcgcgggg cgcctgtaga
      961 gcgacctatg gttcggctgt gcagcgtgcc gtcgagatcg cggcccgggg cgcgagcggg
     1021 cgagtgcgcg ccaccgatgc caccgagctg gccgcggcgg tcgccgccga tcccgacccc
     1081 accgtgcggg ccgttgccct cggtgcgctc gcccgcgccg cacccaagcg ggtcgcggcg
     1141 accgcgtggc gggttgccgc cggcgatgcc gacgccgccg tgcggcgccg cgccgcgcag
     1201 accgcgcccg ccatcggccg gctggtcccc gtcgccatgc tggttgcact gctgcgcgac
     1261 gacgacgcgt gggtcgccga agccgccgcg ttcgcgctcg gcgagcgtga cgatgcgacc
     1321 gcggtggcgc cgctcgagga cgcggcgcgc gggcacgccg acgcactcgt gcgcgaagcc
     1381 gcggttgccg cgctcggcgc gcgcggtgtc ggtcgcgacg cggtgctcga cgcctgccac
     1441 gacaagccgg cgatccggcg acgcgcggtg ctcgcgctcg ccgcgttcga cggtcccgaa
     1501 gtcgaggccg cgttacaggc cgcgctcgag gaccgcgact ggcaggtgcg gcaggcagcc
     1561 gaggacttgt tgcactaacg cacgagcact aacgcgccat gctgcgcgcc atgaacatct
     1621 ctttgagcag gtcgaagttc tcgaggcaac gacccgcgcc gagcacgacg cattccagcg
     1681 gcgcgtcgac gagatgcacg ggcagttcgg tctcttcgct gatgcgtttg tcgagaccgg
     1741 cgagcatgcc gccaccaccg acgaggtgca cgccgttgag gatcaggtct tgggccagct
     1801 ccggcggcgt gcgcgcgagg cacgcgacga ccgcgtcgca gattgcgcgc acttgttcct
     1861 cgatcgcggc ccgcacctct tcgggcagca gcaggatcgt cttcggcagc ccggacatca
     1921 ggtcgcggcc ccggacctcg gccttgtact cgtcgtcgac cgcataggcg gaaccgatcg
     1981 cgagcttgat ctgctcggcc gtcggctcac cgatcgcgat gccgtactcg cgccgcacat
     2041 acgtttggat cgcggcgtcg atgtcgaagg aaccgacccg caatgcttcg agcgcgacga
     2101 cgccaccgag cgagatcacc gcgacctcgg tcgtgccgcc accgatgtcg acgaccatgt
     2161 tgcccatcgg ctcgtggatc gggaggccgg cgccgatcgc cgccgccatc ggttgttcga
     2221 tcaggtacgt ctgggcggct ccggcccgcc gggcggcctc gagcacggct cgctgctcga
     2281 cgtgggtgat cgccgacggc acgcagatga gcacacgcgc gcgatacagg cggctcacac
     2341 ccgcgcgctg gaacagcagg cggatcatgc gctgcgtgat ctcgaagtcg gtgatcgcac
     2401 cgccgcgcaa cgggcgcacc gcgatgatgt agccgggcgt gcgaccgatc atctgccagg
     2461 cttcgtggcc catcgcgagc acttcttggc tgcgttcgtt cagcgcgatg acggttggtt
     2521 cgttgagaat gatcccgcgc ccgcgcgcgt acacgagcgt gttcgcggtt ccgagatcga
     2581 tcgcgaggtc gcgaggcaga gcgacgagcc tctcactccg gcggttgcgg gcgacggttc
     2641 cgggcgtccg gagccagcgc gcgcaacccg cgttcgaact cgtcgatgct gtgctgcatc
     2701 gacgacggtc gccggttccg catcagcacg atcacgatgc cgatcacgct ggcgaccacg
     2761 gcgaacagga ggaactccac ggcgtctccc tacaccgatg tcacgggccg aatgggatgt
     2821 tgctcgacgg cccagtcgga
//
LOCUS       DASUGA010000395        11874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393 k141_9425,
            whole genome shotgun sequence.
ACCESSION   DASUGA010000395 DASUGA010000000
VERSION     DASUGA010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 11874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11874
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9425"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1907)
                     /gene="topA"
                     /locus_tag="VFX21_07820"
     CDS             complement(<1..1907)
                     /gene="topA"
                     /locus_tag="VFX21_07820"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009741725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX5095903.1"
                     /translation="MAKPLVIVESPAKARTIAGYLGDSYTVKASIGHIRDLPGNKDEV
                     PDDKKETHGRLAGINPDDHFDVVYVPHKSKSKVITELRQALKGADELILATDEDREGE
                     AIGWHVLEVLKPKVPVKRMVFHEITPHAIREALEQPRELDLKLVEAQEARRILDRLVG
                     WETSPVLWRVFGRGQAASAGRVQSVAVRLVVERERARMRFRAGNWHDLEGVFVAAAEE
                     QTQEFAAALAELDGRRIAEGRDFDPNTGQIAHGRDVVLLDAAGAEALAGRLRDVPYRV
                     NSVESDPFTERPRAPFTTSTLQQEAGRKLRFTASRAMAVAQRLYERGYITYMRTDSTN
                     LSEQAVTAARTAIREQFGDEYVPNEPRVYRGKVKNAQEAHEAIRPAGDRIRPPDALRA
                     ELDADEQRLYELIWIRTVACQMVDARGRKMTIKLGATSTIGEQAVFRASGKTYDFLGW
                     RRVYVEDVDEDTEVENEARLPAVSEGDAVNCRALQSVGHETKPPARYTEASLVKDLEE
                     RGIGRPSTYAAVIETIQARGYVWRKGTALVPAWTAFAVTNLLEQHFGHLVDYGFTATM
                     EEALDVIARGEGESEKWLDSFYNGNGTPGLRELTSDEMLARIDPRGVSTIPIGADDTG
                     HEIVVRVGRYGP"
     gene            2059..2736
                     /locus_tag="VFX21_07825"
     CDS             2059..2736
                     /locus_tag="VFX21_07825"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX5095904.1"
                     /translation="MIGALDFLDPEKILDWLGPFATIGLLLIIFAESGLLIGFFLPGD
                     SLLFTAGLLSSQDKFGLNIWVLLAGTFGAAVLGDQVGYWFGKRVGPSLFKRPNSRVFK
                     QEYVGRTHDFFERHGPKTIILARFVPIVRTFAPILAGVAGMRYRVFFRFNVIGAAIWA
                     LGVTSAGYILGETVPSIDRYLLPIIALIVLGSVIPPFLEWRKHRKANQAPVELAPRAP
                     QTPEETV"
     gene            complement(2743..5154)
                     /locus_tag="VFX21_07830"
     CDS             complement(2743..5154)
                     /locus_tag="VFX21_07830"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX5095905.1"
                     /translation="MTPVVGAEGGYQEFTLHGGEWVILIGSALTALLAIAVGFFLMRK
                     VLAEDEGTQTMKDIAKAIQEGALAYLKRQFRTIAVILIPLAVVVFLTSTAVKKADGTE
                     ALSFAASGAARTVAFIAGCIMSGLTGFIGMSLAVRGNVRTAAAAKSGSMPRALQVAFR
                     TGGVAGMFTVGLGLIGATTIMMIFQNTSSAILIGFGFGGSLLALFLRVGGGIFTKAAD
                     VGADLVGKVEAGIPEDDPRNPATIADNVGDNVGDCAGMASDLFESYEVTLVASIILGV
                     PAFAAIFPDQPALWAAGALFPLGARALGVLASIVGVFAVRATDKDKSAMAPINRGFLT
                     AGILTVLGTLAFALIYVGNPDGDVSNVGWRMFGAVVGGLVLAQVASRITEYFTSTETK
                     PVQEIAESAATGGPATATLSGISSGLDSSVWAVLAIVGALAVAIGLGGGNIQFSLYLV
                     ALTGMGMLATTGVVVSEDTFGPVADNAAGIAEMSGEFHGEPERIMVSLDAVGNTTKAV
                     TKGFAIGSAVIAAVALFASFIEIIGNEVLGSALDQLERTGTVTVDGITYIGNNVFEAF
                     NVNVADPKVFIGLLIGGSVVFMFSALAIRAVGRTANVVVQEVRRQFADGKIMEGTKRP
                     DYGPVIDICTGASLRELATPALLAVLTPVIVGFGINFFALGAFLAAVILTGQLMANFL
                     SNAGGAWDNAKKYIEDGHYGGKGSDAYRAAVIGDTIGDPFKDTAGPALNPLIKVMNLV
                     SLLILPAVINLEDNDAARFTISGAALVVLLVAIFWSKRGSATLQANEIAIEEADAPAA
                     PAAHV"
     gene            complement(5178..5837)
                     /locus_tag="VFX21_07835"
     CDS             complement(5178..5837)
                     /locus_tag="VFX21_07835"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX5095906.1"
                     /translation="MLESLSKSDREALKAIYRVAKDGEAHTGALADRLGVTPGTVTAA
                     VKRLADRDLVDHKPYRGVELTAAGRQAAIAAIRRHRIVERFLADMLGYAWNEADRLAT
                     AFEHELPQEVEDRLFVALDRPESCPHGFPIPAKETAEMPEMPPLYALEPGDVAVVAVP
                     GSTDPEIVAFLDTLGLRPGVRVEVKEKHPFDGPLVLRVAGKDRTLGATVANQVYVQKS
                     A"
     gene            complement(5904..7799)
                     /locus_tag="VFX21_07840"
     CDS             complement(5904..7799)
                     /locus_tag="VFX21_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX5095907.1"
                     /translation="MFETLKRIFVGRPLATSEQEHQRIPKSIALAVFSSDAISSTAYA
                     TQEILLVVAFGGAGLALGLSRLVPISIVVAILLAIVVTSYRQTIHAYPGGGGSYVVSR
                     ENLGPRFAMVAGASLLVDYILTVAVSICAGVRAITSIPALDGLTNQRVLLCLILIWAI
                     TLANLRGIKESGRIFAFPTYLYIVMLLALVVWGLLHEFELFGLHEFPQISFAEVTKGM
                     PAEERAELFNQLYVSVGTLTLFRLLRGFSSGAVALTGVEAISNGVPAFRRPESRNAAM
                     TLVWMGVILGTLFLGVSILAHHLAPIPLETGESVFSQMGRTVFGEGPLYLVLQIATAG
                     ILTLAANTAYADFPRLSSIIARDGYLPRQLANRGDRLVFSNGVVVLALAASALVVIFG
                     GNETALIPLYAVGVFTSFTLSQSGMVQHHRKEREPRWRLNATVNTVGAIATFIVLMIV
                     AVSKFTEGAWVSIVVVLVVIALFRAIHSHYARVAKGLRTAPDYKPRRMNHTVVVLVGS
                     VHRGALEALAYAKSLAPNYLVAVTVVSDEEEQERIEREWEKRNIQVPLEIVHSPYREL
                     ARPILRFIDEIDARFDNDIVTVVIPEFVVTSWWAHLLHNQSALFLKGRLLFRKGTVVT
                     SVPYHID"
     gene            complement(7883..9742)
                     /locus_tag="VFX21_07845"
     CDS             complement(7883..9742)
                     /locus_tag="VFX21_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX5095908.1"
                     /translation="MLDTVKRIFVGRPLATSEQEHQRLNKTTALAVFSSDAVSSTAYA
                     TEEILFVTALGASSLALGLDVLLPIAIAVAFLLAVVITSYRQTIYAYPKGGAAYVVSR
                     ENHGELPSLVAGASILVDYVLTVAVSVSAGVAAIISIPQFHDAAEHRVEVCLVLIAII
                     TLANLRGLKESGRIFAIPTYGYIVMVTLLVVVGLCQYYFGWFGGFEKVPFDQESFEGL
                     KEAGGTLSLLIIMRGFSSGAVALTGVEAIADGVPAFKRPEPKNAANTLAIMGVILGTL
                     FLGVSLLASHFHPYPSHDVTVFAQMGRAVFGGGWPLWVLQILTAAILTLAANTAYADF
                     PRLSSVIARDGFLPRQLANRGDRLVFSNGIVALALMASLLIVAFGGVTNALIPLYAVG
                     VFTSFTLSQSGMVRHHLRHKEGNYKLHAAISGVGAAATGLVFLIVATTKFTQGAWVPL
                     IVIPAIVVLFKTIKRHYDRVAEGLRVDPGYKPPRMNHTVVVLVGNVHRGTLDALAYAR
                     SLAPNRLLAMTIVSDEEEQERAEKNWSEYNITVPLEIVHSPYRELTRPVMRFVDELDA
                     RHENDLVTVVIPEFVVTGWWAHLLHNQSALLLKGRLLFRKGTVVISVPYHVGN"
     gene            9884..10543
                     /locus_tag="VFX21_07850"
     CDS             9884..10543
                     /locus_tag="VFX21_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003957347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX5095909.1"
                     /translation="MHVVVIGCGRVGTELAGSLNLAGHTVAVVDKNARAFRRLPSNFN
                     GKQVVGFGFDRDHLAEAGIEEAGAFASVTNGDNSNILCARIARETYDIERVVARIYDP
                     RRALIYQRLGISTVATVAWTTDQVLRRLLPTETRHDWADPTGKVGLVERTIPPAAIGK
                     KVGALNNPGRWWLTAVTRFGQAQIVTSDMIGQDGDVLVFISALDALDELQAHIDAGGE
                     H"
     gene            10543..11205
                     /locus_tag="VFX21_07855"
     CDS             10543..11205
                     /locus_tag="VFX21_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX5095910.1"
                     /translation="MRVAIAGAGNVGLFIANDLAGAGHDIQLLEQNPDIAARLHVVAG
                     VEVVIADACEVTSLRAAGLERCDVVVAATGDDEDNLVISLLAKQEFAVPRVIARVNHP
                     KNEWLFNENWGVDLSVSTPHLITALVEEAVSVGRLVRILNFEGGNVGLVEVTLAPDAP
                     VIDKSIVEIDIPRDATIVAVLRGEHVVMPRGDTTFEAGDEVLAMVTREAEEDVTRILT
                     GK"
     gene            complement(11228..11581)
                     /locus_tag="VFX21_07860"
     CDS             complement(11228..11581)
                     /locus_tag="VFX21_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX5095911.1"
                     /translation="MGVRKFVERYTKPVEELDRRDLEQWCDSMSCTSMSALQARHPAR
                     VAGEVRSVRIVPRAGADALEVTVNDGRGSVTGVFLGRRKIGGLMPGRRVVLEGMLAKH
                     DGQNLIYNPVYTLQK"
     gene            11644..>11874
                     /locus_tag="VFX21_07865"
     CDS             11644..>11874
                     /locus_tag="VFX21_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095912.1"
                     /translation="MRPTRWSVLVLVAALALVACSGGDDDNSTEKSEKSTTSSTAVAA
                     DLTLTRGEVAVASAGGDVALDEATQQAVVDAAQ"
BASE COUNT         2059 a         3971 c         3916 g         1928 t
ORIGIN      
        1 ggcccgtagc gaccgacacg gacgacgatc tcgtgaccgg tgtcgtcggc gccgatcggg
       61 atcgtgctca cgccccgggg gtcgatgcgg gcgagcatct cgtcggaggt cagctcccgc
      121 aggcccggcg tgccgttgcc gttgtagaag ctgtcgagcc acttctcgct ctcgccctca
      181 ccgcgggcga tgacgtcgag cgcttcttcc atggtcgcgg tgaagccgta gtcgaccagg
      241 tgcccgaagt gctgctcgag caggttggtg accgcgaacg ccgtccacgc gggaacgagc
      301 gcggtgccct tgcgccacac gtagccgcgc gcctggatcg tctcgatgac cgcggcgtac
      361 gtcgacgggc ggccgatccc gcgctcttcg agatccttga caagactcgc ttcggtgtag
      421 cgcgcgggcg gcttggtctc gtgcccgacc gactgcagcg cgcggcagtt caccgcgtcg
      481 ccttcggaaa ccgccggcag ccgcgcttcg ttctcgacct cggtgtcttc gtcgacgtct
      541 tcgacataga cgcgacgcca accgaggaag tcgtacgtct tgcccgacgc gcgaaacacg
      601 gcttgctcgc cgatcgtcga cgtcgcgccg agcttgatcg tcatcttgcg accgcgtgcg
      661 tcgaccatct ggcacgcgac cgtgcggatc cagatcagct cgtagagccg ctgctcgtcc
      721 gcgtcgagct ccgcgcgcaa cgcgtcgggc ggacggatgc gatcacccgc gggccggatg
      781 gcctcgtgtg cctcttgcgc gttcttgacc ttgccgcggt atacgcgcgg ttcgttgggg
      841 acgtactcgt cgccgaactg ctcgcggatc gcggtgcggg ccgcggtgac cgcctgctcc
      901 gacaggttcg tgctgtcggt acgcatatac gtgatgtagc cgcgctcgta gagacgttgc
      961 gcgacggcca tcgcacgtga cgcagtgaaa cgcagcttgc ggccggcttc ctgctggagc
     1021 gtcgaggtgg tgaacggagc ccgcgggcgt tcggtgaacg ggtcggactc gaccgagttg
     1081 acccggtacg gcacatcgcg cagccgcccg gccagcgctt ccgcgccggc cgcgtcgagc
     1141 aggacgacgt cgcggccgtg cgcgatctga cccgtgttcg gatcgaagtc gcgaccctcg
     1201 gcgatgcggc ggccgtcgag ctcggcgagc gcagctgcga actcctgcgt ctgttcttcc
     1261 gcggcggcga cgaacacgcc ttcgaggtcg tgccagttgc ccgcacggaa gcgcatgcgc
     1321 gcgcgttcac gttcgacgac gaggcgaacc gcaacgctct gcacgcgtcc cgcggacgcg
     1381 gcctggccgc gcccgaagac ccgccacaac accggcgacg tctcccaacc gaccaaacgg
     1441 tcgaggatgc gacgcgcttc ttgcgcttcg acgagcttga ggtcgagctc acgcggttgt
     1501 tcgagcgcct cccgaatcgc gtgcggtgtg atctcgtgga agaccatccg cttgaccggc
     1561 accttcggct tcagcacttc gagcacgtgc cagccgatcg cctcgccctc gcgatcctcg
     1621 tcggtcgcga ggatgagctc gtccgcaccc ttcaacgcct gccgcagctc cgtgatgacc
     1681 ttcgacttcg acttgtgcgg cacgtacacg acgtcgaagt ggtcgtcggg gttgatgccc
     1741 gcgagccggc cgtgggtctc cttcttgtcg tcggggacct cgtccttgtt gccgggcagg
     1801 tcgcggatgt gcccgatact cgccttcacc gtgtacgaat cgccgagata gcccgcgatg
     1861 gtgcgcgcct tggccggaga ctcgacgatc acgagcggct tcgccacctg cacactcccg
     1921 cggctggaaa cgcgtacttc tcggcagacc ggccggtcac cgtagctggc acctcggccg
     1981 gggccgcgaa ttgctcacgt tcagcggcag ccgagggtgc accggccacg gaccgtagcc
     2041 cgtctatggt ggccgcccgt gatcggcgcc ctcgacttcc tcgacccgga aaagatcctc
     2101 gactggttgg ggcccttcgc caccatcggg ctgctgctca tcatcttcgc cgagtcgggc
     2161 ctgctcatcg gtttcttcct gccgggcgac tccctcttgt tcaccgccgg cctgctctcg
     2221 tcccaggaca agttcggcct caacatctgg gtgctgctcg cgggcacctt cggtgccgcg
     2281 gtgctcggcg accaggtcgg ctactggttc ggcaagcgcg tcggcccgtc tctgttcaag
     2341 cgaccgaact cgcgcgtgtt caaacaggaa tacgtcggcc gcacgcacga cttcttcgaa
     2401 cgtcacggcc cgaagacgat catcctcgca cgcttcgttc cgatcgtgcg cacgttcgcg
     2461 ccgatcctcg cgggcgtcgc ggggatgcgg taccgcgtgt tcttccgctt caacgtcatc
     2521 ggcgcggcca tctgggccct cggcgtgacg agtgcgggct acatactcgg cgagacggtg
     2581 ccgagcatcg accgctactt gctcccgatc atcgcattga tcgtgctcgg ttcggtgatc
     2641 ccgccgttcc tcgagtggcg caagcaccgc aaagcgaatc aggcgcctgt ggagctcgcg
     2701 ccgcgagcac cacagacgcc cgaagaaacc gtgtagtacg gctcagacgt gggcggccgg
     2761 cgccgcgggt gcgtccgcct cctcgatggc aatctcgttc gcctgcaacg tcgcgctgcc
     2821 acgcttcgac cagaagatcg cgacgagcaa cacgacgagt gccgcgcccg aaatcgtgaa
     2881 ccgcgcggcg tcgttgtcct cgaggttgat gaccgcgggg aggatcaaca gcgagacgag
     2941 gttcatcacc ttgatgagcg ggttcaatgc cgggccggcc gtgtccttga acgggtcacc
     3001 gatcgtgtca ccgatcacgg ccgcgcgata ggcatcggaa cccttgccac cgtagtgacc
     3061 gtcctcgatg tacttcttgg cgttgtccca cgcaccaccg gcgttcgaca ggaagttcgc
     3121 catcaactga ccggtgagga tcaccgccgc gaggaacgcg cccagcgcga agaagttgat
     3181 accgaagccg acgatcaccg gcgtcaacac cgcgagcaac gccggcgtgg cgagctcccg
     3241 cagcgatgca ccggtgcaga tgtcgatgac cgggccgtag tcgggccgct tcgtgccttc
     3301 catgatcttg ccgtcggcga actgacggcg aacttcctgc acgacgacgt tcgcggtgcg
     3361 gccgaccgca cggatcgcga gcgccgagaa catgaagacg actgagccac cgatcagcag
     3421 accgatgaag accttcggat cagccacatt gacgttgaac gcctcgaaca cgttgttgcc
     3481 gatgtaggtg atgccgtcga cggtgaccgt tccggtccgt tcgagctggt cgagcgcgga
     3541 accgaggact tcgttcccga tgatttcgat gaaggacgcg aacagcgcga ccgcggcaat
     3601 gacggcagag ccgatcgcga agcccttggt taccgccttt gtggtgttcc cgaccgcgtc
     3661 gaggctcacc atgatccgct ccggttcgcc gtggaattcg cccgacatct ccgcgatgcc
     3721 cgccgcgttg tcggcgacgg gaccgaacgt gtcttcggaa accaccacac cggtcgtggc
     3781 gagcatgccc ataccggtga gcgcaaccag gtacagcgag aactggatgt tgccgccgcc
     3841 gagaccgatc gcaaccgcga gcgcgcccac gatcgcgaga accgcccaca ccgacgagtc
     3901 gagaccggag ctgatgcccg agagcgtcgc ggtcgccggc ccaccggttg ccgccgactc
     3961 cgcgatctcc tgcaccggct tggtctcggt cgaggtgaag tactcggtga tgcgcgacgc
     4021 aacctgtgcg agcacgaggc cgccgacgac cgcaccgaac atgcgccaac cgacgttcga
     4081 gacgtcaccg tcggggttgc ccacgtagat cagcgcgaac gcgagcgtgc cgagcaccgt
     4141 gaggatgccc gcggtgagga agccgcggtt gatcggcgcc atcgccgact tgtccttgtc
     4201 ggtcgcccgc accgcgaaca cgccgacgat cgacgcgagc acgccgagcg cacgggcacc
     4261 gagcgggaag agcgcgcccg ccgcccacaa ggcgggctgg tcggggaaga tcgccgcgaa
     4321 cgcaggaacg ccgaggatga tcgacgcgac cagcgtgact tcgtacgact cgaacaggtc
     4381 ggacgccatg ccggcgcagt cgccgacgtt gtcgccgacg ttgtcggcga tcgtggccgg
     4441 gttgcgcggg tcgtcttcgg ggatgcccgc ttcgaccttg ccgacgaggt cggcaccgac
     4501 gtcggccgcc ttggtgaaga tgccaccgcc gacgcgcagg aacagggcga ggagcgaacc
     4561 accgaacccg aagccgatga ggatcgccga gctcgtgttc tggaagatca tcatgatggt
     4621 cgttgcaccg atgagaccga ggccgacggt gaacatgccg gcgacgccgc cggtgcggaa
     4681 cgcaacctgc agcgccctcg gcatcgaccc cgacttcgca gccgcggccg tccgcacgtt
     4741 gccccgcacc gcgaggctca tgccgatgaa gcccgtgagg cccgacatga tgcagcccgc
     4801 gatgaatgcg accgtgcgcg cggcgccgga tgctgcgaac gacaacgctt cggtcccgtc
     4861 ggccttcttc accgccgtcg acgtgaggaa cacgacgact gcaagcggga tgagtatgac
     4921 ggcgatcgtc ctgaactgcc gcttcaggta cgcgagcgca ccctcttgga tcgccttggc
     4981 gatgtccttc attgtctgcg tgccctcgtc ttcggcgagc accttgcgca tcaagaagaa
     5041 cccgaccgcg attgccaaga gcgcggtcag cgccgagccg atgaggatga cccactcacc
     5101 gccgtggagg gtgaactctt ggtagccacc ctcggctcca actactggtg tcatttctct
     5161 ttgttccttg gtaggggcta agcagatttc tggacgtaga cctgattggc cacggttgcg
     5221 ccgagcgttc gatccttgcc tgcgacgcgc agcacgagcg gtccatcgaa ggggtgcttt
     5281 tccttcacct cgacgcgcac tccgggtcgg agtccgagcg tgtcgaggaa ggcaacgatc
     5341 tcggggtcgg tggagcctgg cacggcgaca accgcgacgt cacctggctc cagcgcgtag
     5401 agcggcggca tctccggcat ctccgcggtc tccttggcgg ggatgggaaa gccgtgcggg
     5461 cacgactcgg gccggtcgag cgcgacgaac aagcggtcct cgacctcttg cggcagctcg
     5521 tgctcgaacg ccgtcgcgag gcgatcggcc tcgttccagg cgtagccgag catgtcggcg
     5581 aggaagcgct ccacgatgcg gtgccggcgg atggcggcga tggccgcctg gcgcccggcc
     5641 gcggtcagct cgacgccgcg gtagggcttg tggtcgacga gatcgcgatc ggccaggcgc
     5701 ttgaccgcgg ccgtgaccgt ccccggcgtg acgccgagcc ggtccgcgag tgccccggtg
     5761 tgggcctctc cgtccttcgc gacccgataa atcgctttca gggcttcgcg atcagatttt
     5821 gacaggctct ccagcaagtc agcgatgccg ggaagttaga ttttgatcag ccaaaaagca
     5881 agcggaagac cctggtcccg gcgctaatcg atgtggtacg gcaccgaggt gaccaccgtg
     5941 cccttgcgga agagcaggcg gcccttgagg aagagggcgc tctggttgtg cagcaggtgc
     6001 gcccaccatg acgtcaccac gaactcgggg atgacgacgg tgacgatgtc gttgtcgaag
     6061 cgagcgtcga tctcgtcgat gaagcgcagg atcggccggg ccagctcccg gtacggcgag
     6121 tggacgatct cgagcggcac ctggatgttg cgcttctccc actcccgctc gatccgctcc
     6181 tgttcctctt cgtcggacac caccgtgacc gcgaccagat agttcggcgc gagcgacttc
     6241 gcgtacgcga gcgcctcgag cgcaccgcgg tgcaccgagc ctacgagcac cacgacagtg
     6301 tggttcatac ggcgtggttt gtagtcgggc gcggtacgca gacccttcgc cacgcgtgca
     6361 tagtgcgagt ggatcgcgcg gaacaacgcg atcacgacca gcacgacgac gatcgagacc
     6421 cacgcgcctt ccgtgaactt gctgaccgcg acaatcatca acacgatgaa cgtcgcgatg
     6481 gcgccgacgg tgttgacggt ggcgttcagt cgccagcgcg gttcgcgctc cttgcgatgg
     6541 tgctgcacca tgcccgactg cgacagcgtg aaggacgtga acacgccgac cgcgtacaac
     6601 gggatcaggg cggtttcgtt tccaccgaag atcaccacga gcgcgctggc cgcgagcgcg
     6661 agcacgacga caccgttgct gaacaccagc cggtcgccgc ggttcgcgag ctggcgcggc
     6721 aagtagccgt cgcgggcgat gatcgacgag agccgcggga agtccgcgta cgccgtgttc
     6781 gcggccagcg tcaggatccc cgcggtggcg atctggagca cgagatagag cggtccctcg
     6841 ccgaacacgg tgcggcccat ctgcgagaac accgactcgc cggtctcgag cgggatgggt
     6901 gccagatggt gcgccaggat cgagacgccg aggaacaagg tgccgaggat gacacccatc
     6961 catacgagcg tcatcgccgc gttgcgcgac tccggccggc ggaacgcggg gacgccgttg
     7021 gagattgcct cgacaccggt cagcgcgacg gcgcccgacg agaaaccgcg cagcaaccgg
     7081 aacagcgtga gcgtgccgac cgacacgtac aactggttga agagctctgc tcgttcctcc
     7141 gccggcatgc ccttggtgac ttcggcgaac gagatctgcg ggaactcgtg caacccgaac
     7201 agctcgaact cgtgcagtag accccacacg acgagcgcga gcagcatgac gatgtacagg
     7261 tacgtgggga acgcgaagat gcggcccgac tccttgatgc cccgcaggtt cgcgagcgtg
     7321 atcgcccaga tgaggatcag gcacagcagc acgcgctggt tggtgagccc gtcgagcgcg
     7381 ggaatcgacg tgatcgcgcg cacgccggcg cagatcgaca ccgcaacggt gaggatgtag
     7441 tcgacgagga gcgacgcacc ggcgaccatc gcgaacctcg gaccgaggtt ctcacggctg
     7501 acgacgtagg agccaccgcc acccggatac gcatgaatgg tctggcggta cgacgtgacg
     7561 acgatcgcga gaaggatcgc gacgacgatc gagatcggca ccagccggga caggccgagc
     7621 gcgaggccgg cgccgccgaa cgcgacgacg agcaggatct cttgcgttgc gtacgcggtc
     7681 gaggagatcg cgtccgaaga gaagaccgcg agcgcgatcg acttcgggat gcgctggtgc
     7741 tcctgctccg aagttgccag cggccggccg acgaagatgc gcttgagagt ctcgaacacc
     7801 cggccgtccc actcgaatac gtgaagatgc gagcttggca ggatgcgccg accgcgcccg
     7861 caaggcgcgc gtcaagaaat cgtcagttac cgacgtggta cggcaccgag atgacgaccg
     7921 tgcccttgcg gaacagcagg cgccccttca acaacagtgc gctctggttg tggagcaagt
     7981 gcgcccacca acccgtcacg acgaactcgg ggatcaccac ggtcaccaga tcgttctcgt
     8041 ggcgcgcgtc gagctcgtcg acgaaccgca tcaccgggcg cgtgagctca cggtacgggg
     8101 agtgcacgat ctcgagcgga acggtgatgt tgtactccga ccagttcttc tccgcgcgtt
     8161 cttgttcttc ttcgtcggag acgatcgtca tcgcgagcaa ccggttcggt gcgagcgacc
     8221 tcgcgtaggc caacgcgtcg agcgtgcccc ggtgcacgtt gccgacgagc acgacgacgg
     8281 tgtggttcat gcgcggcggt ttgtacccgg ggtcgacgcg caggccctcg gcgactctgt
     8341 cgtagtgacg cttgatcgtc ttgaacagca cgacgatcgc aggaatgacg atcagcggca
     8401 cccacgcgcc ttgcgtgaac ttcgtcgtcg cgacgatcag gaacacgagc cctgtcgcgg
     8461 cggcaccgac gccactgatc gccgcgtgga gcttgtagtt gccttccttg tgcctgagat
     8521 ggtgccgcac catgcccgac tgcgacagcg tgaacgacgt gaagacgccg accgcgtaca
     8581 gcggtatcag cgcgttcgtc acgccgccga aggcgacgat gagcaggctg gccatcaacg
     8641 ccagcgcgac gatgccgttc gagaacacca gccgatcgcc gcggttcgcg agctggcgcg
     8701 gcaggaagcc gtcgcgggcg atcaccgacg acagccgcgg gaagtcggcg tatgccgtgt
     8761 tggccgcgag cgtcaggatc gcggcggtca ggatctgcag gacccacagc ggccacccac
     8821 cgccgaagac agcccggccc atctgcgcga acaccgtgac gtcgtgactc gggtacggat
     8881 ggaagtggct cgccaacaac gacacgccga ggaacagcgt gccgaggatc acgcccatga
     8941 tcgcgagcgt gttcgccgcg ttcttcggct cgggccgttt gaacgcgggc acgccatcgg
     9001 cgatcgcctc gacaccagtg agcgcgaccg cacccgacga gaaaccgcgc atgatgatga
     9061 gcagcgagag ggtgccgccg gcttctttca atccttcgaa cgactcttga tcgaacggca
     9121 ccttctcgaa gccgccgaac catccgaagt agtactggca caagcccacc acgacgagca
     9181 gcgtcaccat gacgatgtag ccgtaggtgg ggatcgcgaa gatgcggccc gactccttca
     9241 ggccgcgcag gttggccaac gtgatgatcg cgatgaggac gagacagacc tcgacgcgat
     9301 gttcggccgc gtcgtggaac tgcgggatcg agatgatcgc ggcgacaccg gccgacacgc
     9361 tcaccgcgac ggtcaggacg tagtcgacga ggatggacgc gcccgccacg agcgacggca
     9421 gctcaccgtg gttctcacgg ctgacgacgt acgccgcgcc gcccttcggg taggcgtaga
     9481 tggtctggcg gtacgacgtg atgacgactg cgagcaagaa cgcgaccgcg atcgcgatcg
     9541 gcaggaggac gtcgagaccc aacgcgagac tcgacgcgcc caacgcggtg acgaagagga
     9601 tctcctcggt cgcgtatgcc gtggacgaga ccgcgtcgga agagaagacc gcgagggccg
     9661 tcgtcttgtt gagccgctgg tgctcctgct cggatgtcgc gagcggtcgc ccgacgaaga
     9721 tccgcttgac tgtgtcgagc atcctgaggg gctccgccgg tccgaagtgg tcaggcctgt
     9781 ccgccagcat gccatgcgga cctcagccgg gttcggacac agtgtgccga ggcgcgcagg
     9841 tgcgtcgtag catccgcccg tcccgaatgc cgaggaggcg ggcgtgcacg tcgtcgtgat
     9901 cgggtgtggt cgagtcggaa ccgagctcgc cgggtcgttg aacctcgccg gccataccgt
     9961 tgccgtcgtc gacaagaacg cacgcgcgtt ccggcgcctg cccagcaatt tcaacggcaa
    10021 gcaggtcgtc ggcttcggat tcgaccgcga tcatctcgcg gaagcgggca tcgaagaggc
    10081 gggcgcgttt gcatcagtga ccaacggcga caactcgaac atcctgtgcg cccgcattgc
    10141 ccgggagacc tacgacatcg agcgcgtcgt tgcccgcatc tacgacccgc ggcgcgcgct
    10201 catctaccaa cggctcggca tctcgaccgt cgccactgtt gcgtggacaa ctgatcaggt
    10261 gttgcgccgc ttgctgccga cagagacgcg tcatgactgg gccgacccga ccggcaaggt
    10321 cgggctcgtc gaacggacga tcccgcccgc cgcgatcggc aagaaggtcg gcgcgctcaa
    10381 caatcccggt cgctggtggc tgaccgctgt cacccggttc ggtcaggccc agatcgtgac
    10441 ctccgacatg atcggccagg acggcgacgt cctcgtcttc atcagcgcgc tcgacgcgct
    10501 ggacgagcta caggcgcaca tcgacgccgg aggagagcac tgatgcgggt cgcgatcgcc
    10561 ggcgccggca acgtcggatt gttcatcgcc aacgacctcg cgggcgccgg ccacgatatc
    10621 cagttgctcg agcagaaccc cgacatcgcc gcccgcctgc atgtcgtcgc cggcgtcgag
    10681 gtcgtcatcg ccgacgcgtg cgaagtcacg tcgctgcgcg ccgccggact cgaacggtgc
    10741 gacgtcgtcg tcgccgcgac cggtgacgac gaagacaacc tcgtcatctc gctgctggcg
    10801 aagcaggagt tcgcggtgcc gcgcgtcatc gcgcgcgtca accatccgaa gaacgagtgg
    10861 ctgttcaacg agaactgggg cgtcgacctc tcggtctcga cgccgcacct catcaccgcg
    10921 ctcgtcgaag aggccgtatc ggtcggccgc ctcgtccgca tcctcaactt cgaaggcggc
    10981 aacgtcggtc tcgtcgaggt gacgctcgcc cccgacgcgc cggtcatcga caagtcgatc
    11041 gtcgagatcg acatcccgcg cgacgcgacg atcgtcgccg tcctgcgtgg tgagcacgtc
    11101 gtcatgccgc gcggtgacac gacgttcgaa gcgggcgacg aggtgctggc catggtcacc
    11161 cgcgaggccg aggaagacgt cacgcgcatc ctcaccggca agtagccgtc gtgcggcgcg
    11221 ttcggcgcta cttctgcagg gtgtagacgg ggttgtagat caggttctgg ccgtcgtgct
    11281 tggcgagcat gccttcgagc acgacgcgcc gtcccggcat gaggccgccg atcttgcgcc
    11341 ggcccaggaa cacacccgtc accgagccgc gcccgtcgtt gacggtcact tcgagcgcgt
    11401 cggcacccgc tcgcggcacg atccgcaccg accgcacttc acccgcgacc cgggccggat
    11461 gccgggcctg cagcgccgac atcgaggtgc acgacatgct gtcgcaccac tgttcgaggt
    11521 cgcggcgatc cagctcctcg actggcttgg tgtagcgctc gacgaatttt cgtacgccca
    11581 aaacagatcc cccatccgga ggtgcacgag tttaacgctc tcctccctta cgctattccc
    11641 ccgatgcgac ccacccgatg gtcggtgctg gtcctcgtcg ctgccctcgc gctcgtcgcg
    11701 tgctctggtg gtgacgacga caattccacc gaaaagtccg agaaatcaac gacgtcctcg
    11761 accgcggttg ccgcggacct gaccctcacc cggggcgagg tcgcggtggc cagcgcgggc
    11821 ggcgacgtcg cgctcgacga ggcgacacaa caagcagtcg tcgacgcggc gcag
//
LOCUS       DASUGA010000396         5607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1566618, whole genome shotgun sequence.
ACCESSION   DASUGA010000396 DASUGA010000000
VERSION     DASUGA010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5607
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566618"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VFX21_07870"
     CDS             <1..484
                     /locus_tag="VFX21_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095913.1"
                     /translation="VAALAVVGAIGGVLVWNRPPARIYLGDAGSYLIGTALALLLGGA
                     LAPGQPVATGAAALLLVGVPVIDTSVAIVRRFRAHRPLLQGDRGHVYDQLVDRGWRPI
                     AATGACVGAQAVLAAAAIAIAELPAGAAVVTVAIVIALVGGVVLTAFTTPGSWARDLE
                     "
     gene            484..1425
                     /locus_tag="VFX21_07875"
     CDS             484..1425
                     /locus_tag="VFX21_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095914.1"
                     /translation="MDIFGTADFRAGLAAGAVGAVCVLVAAAVVRRWRPRALPIAGAG
                     VAFTGASFVAVALRHDVPAPVPVAVAGVGSAVWLLARRHAPPWLGVFAAVPFAALLAF
                     ACDLATPAWFRLVVFGTVCLGVPLIASFEATWGTGAPVGGLLALSVLGIAFAVPDTEE
                     IASLAGTAIVIAVAAWPFVPVRIGAGGAAAALAWTTWVAAFDARGRPAALLGAVGCFG
                     LLVGLALVDPIARRVLRAHPWSVWTAIVLTAVHAVVVALMSRVAGTEASVGRAAMVVL
                     VVAVPALVVGLPLAARRPPRERVASDSGSRLRVDVER"
     gene            complement(1384..4572)
                     /locus_tag="VFX21_07880"
     CDS             complement(1384..4572)
                     /locus_tag="VFX21_07880"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5095915.1"
                     /translation="MEESTETPLTMSAGGSTEDGSLPRAVYRFASRLDNASLRLLDAG
                     VIVVSWLLAYLAGYEGHFPRHSAIGPLVFLAIPLVIQLVANHAAGLYGPVWKYASVEE
                     AARVVAAVAIGAAASTAVVGVLAKVTDTMLPVFTTPPVAALLMLLGCGGIRFQARLFA
                     LERGRVGRTDNKLRTLIVGAGSSGVSLAVELGKDARSDLRVVGFVDDTPHLSGRTVRG
                     VRVLGRTSDLERICKEEKIERVIIALPNISRAETQAMMDLALKTDAQVKVLPRASDLV
                     NGPLLRSLRDIDVADLLGREHAPVDSEEIAEYLEGATVLVTGAGGSIGSEIARQVARF
                     QPGRLLLLDRDETLLHEVVSDSLADAVPVLGDLRNQTRIREIFERYRPDVVFHAGAQK
                     HVPILERHPAEAVHTNVLGTWWLATIAAECGCGRFVHVSTDKAANPCSVMGSTKRAAE
                     LVVHEVGDQFDLPFAAVRFGNVLGSRGSVVPTFLRQILAGGPVTVTNPEMTRYFMTIP
                     EAVSLVLQAGAMADEGNVFLLEMGKPVSIIDLAKQMIRLAGLRPGEDIEIKITGPRPG
                     ERLHEQLHDDAEIVDATRHPSIKGVVPKSPPDRETVFFFLALLEQACTESNNDAAIAS
                     LLGQLLRHSGIDCHLELESAPSTASGRITKDLRPTRATRLPALLGGRPRFSEQLPFAR
                     PARPPLEQVFKRIEPSYESGMLTNGPLVRELEARAAQLLEVEHVVAVSSCTSGLMLTL
                     QAVTGGRTGPVVLPSFTFSASAHAVAWNGRTPSFVDCEADSLQISTEDAAEALDGASA
                     ILATHVFGAPCDVEQLTKLAKAAGVPLVFDAAHAFGARFDDRPVGGHGVAEVFSLTPT
                     KVLVAGEGGLVATNDEMLAELLRIGRDYGNPGDYNTRFAGLNARMSEFHAAMALESLE
                     MLDTSLMRRRQLAARYQDHLADVPGVAFQTVRPGDLSTYKDFSIVIDPDKFGLTRDKL
                     VSVLTAEGIDTRKYFDPPVHRQHTYSRLEPVDLPVTDSVASSVLSLPIYPGLRGDEIR
                     RVAESIRTAHTHAVELDEALSFHVDTESRPRV"
     gene            complement(4749..>5607)
                     /locus_tag="VFX21_07885"
     CDS             complement(4749..>5607)
                     /locus_tag="VFX21_07885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5095916.1"
                     /translation="PVVVWQRILPFPPSIWRPGRRQFWQAVTRRIDAAVALTNDDRTE
                     LRRLGFTKTVHVIPNFRRLSRFAEVERQQAEARLRDRLGVGADVRLLGLVGHLIAQKR
                     PERALDVLRGVREDGIDAHLVVAGDGPLRQALVGACEREDLTPFVHVLGTVSDVGAVL
                     GGLDALLLTSDSEGIPGIVIEAQMCALPVVTFDLGGIATVVVNNVTGAIVAAGDVAAM
                     ARRTSELLRDETRNARMRKAAREHAAVFATERVARDYEKLLRELTAQRHPPSFGESGQ
                     MARAKSGDL"
BASE COUNT          818 a         1867 c         1947 g          975 t
ORIGIN      
        1 tgttgccgca ctcgcagtcg tcggcgcgat cgggggcgta ctcgtgtgga accgcccgcc
       61 cgcgcgcatc tatctcggtg acgccggcag ctatctcatc gggacggcgc tcgcgctcct
      121 gctcggaggt gcgctcgcgc ccggtcaacc cgtcgcgacg ggcgcggccg cgttgctgct
      181 cgtcggcgtg cccgtcatcg acacttccgt cgcgatcgtt cgccgttttc gcgcacatcg
      241 tcccctgctc cagggcgacc gcggacacgt gtacgaccag ctcgtcgacc gaggatggcg
      301 gccgatcgcc gcgaccggtg cgtgtgtcgg tgcacaggcc gtgttggcgg ccgcggcgat
      361 tgcgatcgcg gagcttcccg ccggcgcggc agtcgtgacc gtggccatcg tgatcgcact
      421 cgtcggcggt gtcgtgctga ccgcattcac cacgcccggc tcgtgggccc gagatctgga
      481 atagtggaca tcttcgggac tgcggacttt cgcgccggtc tcgcggccgg tgccgtcggc
      541 gctgtgtgcg ttctcgtggc tgctgcagtc gtccggcggt ggcgtccgcg cgcgttgccg
      601 attgccggtg cgggagttgc gttcacgggt gcgtcgttcg ttgccgtcgc ccttcgccac
      661 gacgtgccgg caccggtgcc cgtcgccgtg gccggcgtcg ggtcggccgt ctggttgctg
      721 gcccgtcgcc acgccccgcc atggctcggt gtgttcgcgg cggtgccgtt cgcggcgctg
      781 ttggccttcg cgtgcgacct tgcgaccccg gcctggttcc gtctcgtcgt cttcggcacc
      841 gtctgccttg gggtaccgct catcgcgtcc ttcgaagcca cgtgggggac gggcgcgccc
      901 gtcggtgggt tgctggcgct cagcgtcctc gggatcgcgt tcgccgttcc ggatacggag
      961 gagatcgcaa gcctggccgg cacggcgatt gtgattgccg tcgccgcgtg gccgttcgtg
     1021 ccggtgagga tcggtgccgg tggggccgcg gccgcgctcg cgtggaccac atgggtcgcc
     1081 gcattcgatg ctcgcggccg cccggccgcg ctgctcggag ccgtcggatg cttcgggctg
     1141 ctggtcggcc tcgcgctcgt cgacccgatc gcgcgccgcg tgcttcgtgc gcacccgtgg
     1201 agcgtgtgga cggcgatcgt gttgacggca gtgcacgcgg tcgtcgtcgc gctcatgtcg
     1261 cgggtcgcgg gtaccgaagc gtcggtcggc cgcgcggcga tggtcgtgct cgtcgttgcc
     1321 gtgccggcgc tcgtcgtcgg gctgccgctt gcggcgcggc ggccgccgcg cgaacgcgtg
     1381 gcgtcagact ctgggtcgcg actccgtgtc gacgtggaac gatagggcct cgtcgagctc
     1441 gaccgcatgc gtgtgcgcgg tgcggatgga ctcggcgacg cgacggatct cgtcgccgcg
     1501 gaggccgggg tagatcggca gggacagcac gctggacgcg accgagtcgg tgacgggcag
     1561 gtcgacaggc tcgagacgcg agtacgtgtg ctgccggtgc acgggcgggt cgaagtactt
     1621 gcgggtgtcg ataccttcgg cggtgagcac cgacaccagc ttgtcgcgcg tcagaccgaa
     1681 cttgtccggg tcgatgacga tcgagaagtc cttgtacgtc gacaagtcac cgggtcgcac
     1741 ggtttggaag gcgacacccg gcacatctgc gaggtggtcc tggtaccgcg ccgcgagctg
     1801 gcgccgccgc atcaatgacg tgtcgagcat ctcgagcgac tcgagcgcca tcgcggcgtg
     1861 gaactccgac atccgcgcgt tgaggcccgc gaaacgcgtg ttgtaatcgc cggggttccc
     1921 gtaatcgcgt ccgatgcgaa gaagttcggc gagcatctcg tcattcgtcg caacgagtcc
     1981 gccctcgccg gcgacgagca ccttcgtcgg cgtgaggctg aagacctccg cgacgccgtg
     2041 gccaccgacc ggccggtcgt cgaaccgcgc accgaatgcg tgagcggcgt cgaacacgag
     2101 cggcacaccc gcggccttcg ccagcttcgt cagttgctcg acatcgcacg gcgcgccgaa
     2161 cacgtgcgtc gcaaggattg cgctcgcgcc gtcgagagct tcggcggcat cttcggtgga
     2221 gatctggagc gagtcggctt cgcagtcgac gaagctcggt gtgcggccgt tccatgcgac
     2281 ggcgtgcgcc gacgccgaga aggtgaagct cggcaggacg accggacccg tacgaccacc
     2341 ggtcaccgcc tggagcgtca gcatcaagcc tgaggtgcac gagctcacgg cgacgacgtg
     2401 ctcgacctcg agaagctgcg cggcgcgcgc ttcgagctct cgaacgaggg ggccgttcgt
     2461 cagcatgccg ctttcgtacg acggctcgat gcgcttgaag acttgttcga gcggcggacg
     2521 ggcgggacgg gcgaatggca actgctcact gaatcgcggc cgtccgccca ggagtgcggg
     2581 gaggcgcgtg gcccgcgttg gccgtaggtc cttggtgatg cgcccacttg ccgtgctcgg
     2641 cgcggactcg agctcgagat ggcagtcgat cccggagtgg cggagcaact ggccgagcag
     2701 gctggcgatc gccgcgtcgt tgttgctctc ggtgcacgcc tgctcgagca acgcgagaaa
     2761 gaagaacacc gtttcgcgat cgggcggcga cttggggacc acacccttga tggacgggtg
     2821 tcgtgtcgcg tcgacgatct ccgcgtcgtc gtggagttgc tcgtgcaacc gctctccggg
     2881 gcgcgggccg gtgatcttga tctcgatgtc ttctcccggc cgcaaaccgg cgagtcggat
     2941 catctgcttg gcgaggtcga tgatcgaaac gggtttgccc atctcgagaa ggaagacatt
     3001 gccctcgtcc gccatcgcgc cggcttgcag aaccaggctg acggcctcgg ggatcgtcat
     3061 gaagtaccgc gtcatctcgg ggttggtcac cgtgaccgga ccgccggcga ggatctgacg
     3121 caggaaggtc ggaacgacgc tgccgcggct gcccaacaca ttgccgaagc gcaccgctgc
     3181 gaacggcaga tcgaactggt caccgacctc gtgcacgacc aactcggccg cgcgctttgt
     3241 cgatcccatc accgagcacg ggttcgcggc cttgtcggtc gacacgtgca cgaaccgtcc
     3301 gcatccgcat tccgcggcga tcgtggcgag ccaccacgtg ccgagcacgt tcgtgtgcac
     3361 ggcttcggcc gggtgccgct ccaggatcgg aacatgcttt tgcgcgccgg cgtggaacac
     3421 gacgtcgggc cggtagcgct cgaagatctc gcggatccgt gtctggttgc gcaggtcgcc
     3481 gagcaccggg accgcgtcgg ccagcgaatc gctgacgacc tcgtgcagca gcgtctcgtc
     3541 ccggtcgagc aacagcagcc ggccgggttg gaaccgagcg acctggcggg cgatctcgct
     3601 gccgatcgag cctccggcgc cggtcaccag aacggtcgcg ccttcgaggt actcggcgat
     3661 ctcctcggag tcgacaggtg cgtgctcccg cccgagtagg tccgcaacat cgatgtcgcg
     3721 caggctgcgc agcagcggtc cgttgacgag atccgacgct cggggcaaca ccttgacctg
     3781 cgcgtcggtc ttcaacgcga ggtccatcat cgcctgcgtc tcggcccgac tgatgttcgg
     3841 caacgcgatg atcacgcgct cgatcttctc ttccttgcat atgcgctcga gatccgacgt
     3901 acgtcccaac acgcgcacgc cgcgcacggt acggccggac aggtgtggtg tgtcgtcgac
     3961 gaagccgacg acgcgcaagt cgctgcgtgc gtccttgccg agctcgaccg cgagcgagac
     4021 acctgagctg cccgcgccca cgatgagtgt gcgcagcttg ttgtcggtgc gtccgacgcg
     4081 cccgcgctcc agcgcgaaca agcgcgcctg gaagcggatg ccgccgcagc cgagaagcat
     4141 caacagcgcc gcgaccggcg gtgtcgtgaa gacaggcagc atcgtgtcgg tgaccttggc
     4201 gagcacgccg acgactgcgg tcgacgccgc ggcaccgatg gcgacggcgg cgacgacacg
     4261 agccgcctcc tcgacggagg cgtacttcca caccggtccg tagaggccgg cggcgtggtt
     4321 cgccaccagc tggataacta gcggaatggc aaggaagacg agcggcccga tggccgagtg
     4381 gcgggggaaa tgcccttcgt agcccgcgag gtacgcgagg agccacgaca cgacgatgac
     4441 gcccgcgtcg agcagccgca gtgatgcatt gtcgaggcgg cttgcgaatc ggtagacggc
     4501 gcgcggcagc gaaccgtctt cagtcgagcc tcccgcgctc atcgtgagcg gcgtttccgt
     4561 ggattcctcc acgcatctcc cccagaccga gatgttcccg ccacccgatg tgcatcctgc
     4621 ttgctgctcg ctgcgtactg ccgccccgcg acggattgct ccgcgctccg acagtcacaa
     4681 atccttgctt gttctcgccg gtcgaggcaa cacgcaaact gagtatgcgt ttacaagtca
     4741 cgtaactttt acaagtcacc tgactttgcg cgggccattt gcccggattc accgaatgat
     4801 ggcggatgac gttgcgcggt gagctcgcgc aagagctttt cgtagtcgcg cgcgactcgt
     4861 tcggtcgcaa agaccgcagc gtgttcgcgc gccgctttcc tcatgcgcgc gttgcgtgtt
     4921 tcgtcccgca ggagctcgga cgttcgtcgt gccatcgcgg cgacgtcacc ggccgcgacg
     4981 atcgcgccag tgacattgtt cacaacgacg gttgcaatgc cgccgaggtc gaaggtcacg
     5041 acgggcagtg cgcacatctg agcctcgatc acgatgccgg ggattccctc cgagtcgctc
     5101 gtgaggagaa gcgcgtcgag gccccccaag actgctccga cgtcgctgac cgttccgagg
     5161 acgtggacga acggcgtgag atcttcgcgc tcgcacgcgc cgacgagtgc ctgtcgcagg
     5221 ggtccgtcgc cggcgacgac gaggtgcgcg tcgatgccgt cttcgcgcac tccccgaagt
     5281 acgtcgagcg cccgctcagg ccgcttctgc gcgatcaggt gaccgacgag tccgagcagc
     5341 cgcacgtcgg caccgacgcc gagccggtca cgcagccgcg cttctgcctg ctgccgctcc
     5401 acctcggcga agcgcgacag tcggcgaaag ttcggaatga cgtgcacggt cttcgtgaaa
     5461 cccaagcgcc ggagctcggt tcggtcatca ttcgtgagcg caaccgcggc gtcgattcgg
     5521 cgcgtcactg cctgccagaa ctggcggcgc ccgggacgcc agatactcgg tgggaaggga
     5581 agaatccgtt gccatacgac caccggt
//
LOCUS       DASUGA010000397         7734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2073236, whole genome shotgun sequence.
ACCESSION   DASUGA010000397 DASUGA010000000
VERSION     DASUGA010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7734
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2073236"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VFX21_07890"
     CDS             <1..186
                     /locus_tag="VFX21_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095917.1"
                     /translation="DGREVASFEAFWEDQAREEFGDLGPAEEAGPAPSGGRGGDRGGR
                     GGGRGGRGGRGRGGPRR"
     gene            194..1447
                     /locus_tag="VFX21_07895"
     CDS             194..1447
                     /locus_tag="VFX21_07895"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5095918.1"
                     /translation="MTQAASDVQRAQLPSGLRVVTESLPGLRSVTIGAWVGSGARDES
                     LPEWGASHFLEHLLFKGTPERSAKDIARAVESVGGEMNAFTTHEQTVFYVRVPDTHLA
                     LALDILADVLWHPAFRENDVDAERQVILEEIGMRDDTPDDLVHDVFARAMYPDHILGR
                     EVLGSDETITAMTRDQIATYHGAHYHPSNMVLAAAGNLVHDDVLSVIASKFPGDGGAR
                     PTRPRAEPPSVVPLEVVHRDSEQAHVVLGVRAIPAMDPDRYALTVLNQAFGGGMSSRL
                     FQEIREERGLAYSVYSYRAAFDDNGYLAVYAGTSPERIHETLDVVNVELDRLVRDGLP
                     ADELDAAKGHLTGSLTMSLETSASRMRRIGRAEQVEGDVPTLDELVARVDAVTDDDIN
                     RVIERVLKDADRTMAIVGPHEASEF"
     gene            1447..2214
                     /gene="dapB"
                     /locus_tag="VFX21_07900"
     CDS             1447..2214
                     /gene="dapB"
                     /locus_tag="VFX21_07900"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX5095919.1"
                     /translation="MINVGVFGAGGRMGATVCGAVSGASDMELVAAVDPFHAGEEVCG
                     VTIAGDKGALKDAGAEVVVDFTVIDAARENVQWCAENGVHAVVGTTGFSDEELSQFTA
                     AFAKSNANAVIAPNFAIGAVLLMRFAELAAPHFDSAEIIELHHENKIDAPSGTAMYTA
                     QRIAAASKDWLDDPTTKVVAEGARGGSVEHIPIHSVRLRGLVAHQEVLFGTTGQTLSL
                     RHDTIDRSSFMPGVLLAVRKVGATPGLTLGLDALLGF"
     gene            complement(2180..2848)
                     /locus_tag="VFX21_07905"
     CDS             complement(2180..2848)
                     /locus_tag="VFX21_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095920.1"
                     /translation="MVSPPSRAGISDAGRRRVDAALARHGMLLVQGQDVVPSLADLLA
                     RRAITTRGYSWDYEPAWRMRRELEARSDIASVKLLRGKVTIIAPRLWPAVRAVADAAR
                     DAVDSPALREIERTPAITVERLMAATGLDKKATRKETRDLERWCCAFSRERDDVEYHT
                     HEQAWFPWSAHKVARSRRGRACSFDDAVALLHATAGGRESAARLFPVVAVTLRNPGAR
                     RARA"
     gene            2894..3634
                     /locus_tag="VFX21_07910"
     CDS             2894..3634
                     /locus_tag="VFX21_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5095921.1"
                     /translation="MAPLLSVEDVRVEFGGIVALVDLSFTIDEGQICALIGPNGAGKT
                     TFFNCVSRLYQTSSGRITFAGNELTRLPPYRMAKLGIARTFQNLALFPALTVLENVMV
                     GGNSLGGSSATRRREARAILDRFSLAHLADHHATGLPYGTLKRIEFARALAAKPRLLM
                     LDEPAAGLTHSEVDELGELIRSIRDEFSLSVLLVEHHMAMVMAISEKIVVLDFGSKIA
                     EGGAEEISGNPRVIEAYLGAPPDTGTAG"
     gene            3631..4353
                     /locus_tag="VFX21_07915"
     CDS             3631..4353
                     /locus_tag="VFX21_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5095922.1"
                     /translation="MIDAGSVLLQVAELRAGYGPVQVLHGVDFAVHEGEVVVILGANG
                     AGKTTTLRAISGMIHARGRVEFAGQSLLGLKPEQVTRAGIAHVPQGRGTIADLTVDEN
                     LRIGAYTRKDRGVDDDIDRWYTMFPRLGERRTQAAGSMSGGEQQMLAISRALMSRPKL
                     LLLDEPSLGLAPIVTQGLFRVLGELNRDHGTSMLIVEQNAQLALGIARRGYVLETGSI
                     AVSGEAQALSTNDDVRRAYLGI"
     gene            4353..5240
                     /locus_tag="VFX21_07920"
     CDS             4353..5240
                     /locus_tag="VFX21_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003994917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5095923.1"
                     /translation="MDLFFAQLINGIGAGVVYASLALALVLIFRTTGILNFAQGEMAL
                     VSTYLTWKFTDYGWPVWLSIVASVAISFVGGALIERIIIRPVERSSPLVIVIVTIGLF
                     LALNSLAQVIFGTDNKVMPRFYEFHVWELAGIRVSSDTVVLVAVLAVECALLWLLLQR
                     TKIGLALRAVASNAESSRLVGVSVGTMLMFGWGLSAALGALAGSLVIPTTGSQSLTAG
                     SMQAILFYAFAAAAIGGFDSPVGAVVGGMIVGVTQALTIEYVDALDGIESVVPLALIL
                     LVLLFKPSGLFGKVQVERV"
     gene            5237..6316
                     /locus_tag="VFX21_07925"
     CDS             5237..6316
                     /locus_tag="VFX21_07925"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5095924.1"
                     /translation="MTVSRVVKGIVFLVLLGALLYVPQYYEATTVNHFTTAIYVAIAA
                     LGLNILTGYNGQVSIGHGVFFALGSYTTAILMRDRDWPFLATIPVVLVVCFVVGGLVG
                     FPALRVKGLYLALVTLGVAVVFPDLARKFVNRDGIWDPNGEPGVVQLSIVRNLRPPNW
                     YTWFPDSIADHFDEWIHDRDQWAFYCAITLGLVLLLLVWLIVRSRFGRALIAVRDHEA
                     AAETVGINLARVKVTAFALSALYAGVAGSIAVLLDRSASVDKVEVFQDSILFLVAVVI
                     GGTATVIGPIVGAFVLVFFQEWANEQSTLQDWLGDERAKLFSPALFGIALIVSMYFLP
                     DGIVGGGRRLWRRVRRRHPVAESVN"
     gene            6340..7731
                     /locus_tag="VFX21_07930"
     CDS             6340..7731
                     /locus_tag="VFX21_07930"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5095925.1"
                     /translation="MRVRSAKPFRLLALAVAAATLFAACGDGRDDDDSSSDTTAASGT
                     PTSAGEGGGGAATFDIDTADCETDPASVTIEGDTIKLGTSLPQSGIYAAFAEILRGEQ
                     AYIQYLNDDLGGVEVAGKTYKVELVAKDDGYNSDQTVANVDSLIADDDVFALFNVVGT
                     KNNLAIRDSVQTNCIPNLFAATGSPAWGNPEYPWMLGTYLVPYPLEAQALVNYLEDNN
                     PTASLAILRADDDFGRAYADTLKALIEGTDITIKQEETYDPEGAEVAAQVTSLAASGA
                     DVFVLGATLLACPSALNEMGKAGWKPSVVYMSGTCTSKTLMAAAGDNGAGVLSVSPLM
                     DPNDPQFASDEAMTLYKEKIAQYGNGADATNGIVAYGWSAGAALQKSLEAASAPNRLA
                     VMQAARTLDLSDVGLQLPGSEWVVGQDDWFLGEDFNLVQYDLAGKYFKPVGDLIKENG
                     ETEKITPENLING"
BASE COUNT         1212 a         2615 c         2635 g         1272 t
ORIGIN      
        1 gacggccgtg aggtcgcgtc gttcgaggcg ttctgggaag accaggcgcg cgaggagttc
       61 ggcgacctcg gcccggccga agaggcgggc ccggccccgt ccggcggccg gggcggcgac
      121 cgtggtggtc gcggcggggg acgaggcggt cgcggtggac gcggtcgcgg cggcccgcgc
      181 cgataatcgc tacgtgacgc aagctgcttc cgacgtccag cgggcgcaac tgccgtcggg
      241 tctgcgcgtc gtcaccgagt cgctgcccgg tctgcgctcg gtgacgatcg gcgcgtgggt
      301 gggcagcggc gcccgcgacg agtcgctccc cgagtggggt gcgagtcact tcctcgagca
      361 cctgttgttc aagggcaccc ccgaacgcag cgccaaggac atcgcgcgcg cggtcgagtc
      421 ggtgggcggc gagatgaacg ccttcacgac gcacgaacag accgtgttct acgtgcgcgt
      481 ccccgacacg cacctcgcgc tggcgctcga catcctcgcc gatgttttgt ggcacccggc
      541 gttccgtgag aacgacgtcg acgcagagcg tcaggtgatc ctcgaagaga tcggcatgcg
      601 cgacgacacg ccggacgatc tcgtccacga cgtgttcgcc cgcgcgatgt atcccgatca
      661 catcctcgga cgcgaagtgc tcgggtccga cgagacgatc accgcgatga cccgcgacca
      721 gatcgcgaca tatcacggcg cgcactacca cccgtcgaac atggtgctgg ccgcggcggg
      781 caacctcgtg cacgacgacg tgctctcggt catcgcatcg aagttccccg gtgacggggg
      841 cgcccgcccg acacggccgc gcgccgagcc gccgtcggtg gtgccgctcg aggtcgtgca
      901 tcgtgactcc gagcaggcgc acgtcgtgct cggcgtgcgt gcgatccccg cgatggaccc
      961 cgaccgttac gcgctgaccg tgctgaacca ggcgttcggc ggtgggatgt cgtcgcggct
     1021 gttccaggag atccgagaag agcgtggcct cgcgtactcc gtgtactcgt accgcgccgc
     1081 gttcgacgac aacggctacc tcgccgtgta cgcggggacg tcgcccgaac gcatccacga
     1141 gacgctcgat gtcgtgaacg tcgagctcga ccgcctcgtg cgcgacgggc tgccggccga
     1201 cgagctcgat gcggccaagg gccatctcac cggttcgctc acgatgtcgc tcgagacatc
     1261 ggccagccgg atgcgccgca tcggtcgcgc cgagcaggtc gagggcgacg tcccgacgct
     1321 cgacgagctc gtggcgagag tcgacgccgt caccgacgac gacatcaatc gcgttatcga
     1381 gcgcgtgctg aaggacgcgg atcgtacgat ggccatcgtc gggccacatg aagcaagcga
     1441 gttctgatga tcaacgtcgg ggtgttcgga gcggggggcc ggatgggcgc caccgtgtgc
     1501 ggcgcggtgt caggtgcgag cgacatggag ctcgtcgctg cggtggaccc gtttcacgcc
     1561 ggtgaagagg tgtgcggcgt cacgatcgcg ggcgacaagg gcgcactgaa agacgccggg
     1621 gccgaggtcg tcgtcgactt cacggtcatc gacgccgcac gcgagaacgt gcagtggtgc
     1681 gccgagaacg gcgtgcatgc agtggtcggc acgacgggct tctccgacga agaactgtcg
     1741 cagttcacgg ctgcgttcgc gaagagcaac gccaatgcgg tgatcgcgcc caacttcgcg
     1801 atcggcgcgg tgctgctgat gcgcttcgcc gagctcgcgg cgccgcactt cgatagcgcc
     1861 gagatcatcg agctgcacca cgagaacaag atcgacgcgc cgtcgggcac cgcgatgtac
     1921 accgcacagc gcatcgcggc cgcatcgaag gactggctcg acgacccgac cacgaaggtc
     1981 gtcgccgagg gtgcacgcgg cggcagcgtc gagcacatcc cgatccactc cgtacggttg
     2041 cggggcctcg tcgcacacca agaggtgctg ttcggtacga cggggcagac gttgtcgctg
     2101 cgccacgaca cgatcgaccg ctcgtcgttc atgccgggcg tcctgctcgc ggtgcgcaag
     2161 gtcggcgcga cgccgggcct cacgctcggg ctcgacgcgc tcctgggttt ctgagcgtca
     2221 ccgcgaccac cgggaacaag cgggccgcgc tctcgcggcc acccgcggtt gcgtgcagca
     2281 acgccaccgc gtcgtcgaac gagcatgctc gtccacgccg tgaacgggcg accttgtgcg
     2341 ccgaccacgg gaaccacgct tgctcgtgcg tgtggtactc gacgtcgtcg cgttctcggc
     2401 tgaacgcgca acaccaccgt tcgagatcgc gcgtttcctt tcgcgtcgcc ttcttgtcga
     2461 gacccgttgc ggccatcagc cgctcgactg tgatcgccgg cgtccgctcg atctcgcgca
     2521 acgccggcga atcgacggcg tcgcgcgcgg catcggcaac cgcgcgcacg gcgggccaca
     2581 gacgcggcgc gatgatcgtc accttgcccc gcaacagctt gaccgacgca atatcggagc
     2641 gcgcttcgag ttcgcggcgc atgcgccagg ccggttcgta gtcccacgag tacccacgcg
     2701 tcgtgatcgc gcggcgcgcg agcaagtcgg ccaggctcgg gacgacgtcc tggccctgca
     2761 cgagcagcat cccgtgacga gccagcgcgg catcgacacg gcggcgcccg gcatccgaga
     2821 ttcccgctcg cgacgggggc gaaaccacgc gcgagatagt aggtttcgcc accgctgcca
     2881 accggggggt tcagtggcgc cgctgttgtc ggtcgaagac gtgcgggtcg agttcggcgg
     2941 catcgtcgcg ctcgtcgacc tgtcgttcac gatcgacgaa ggccagatct gcgcgttgat
     3001 cggtcccaac ggcgcgggga agacgacgtt cttcaactgc gtcagccgcc tgtaccaaac
     3061 cagctcgggc cgcatcacgt tcgccggcaa cgagctcacg aggctgccgc cgtaccgcat
     3121 ggcgaagctc ggcatcgccc gcacgttcca gaacctggcg ctgttcccgg cgctgacggt
     3181 gctcgagaac gtcatggtcg gcgggaactc gctcggcggc tcgagcgcga cgcgccgccg
     3241 ggaggcgcgc gcgatcctcg accggttctc gctcgcgcac ttggccgacc atcacgcgac
     3301 gggtctcccg tacggcacgc tgaagcgcat cgagttcgcg cgggcgctgg ccgcaaagcc
     3361 gcgactgctg atgctcgacg aaccggcggc cggcctcacc cactccgagg tcgacgagct
     3421 cggcgagctg atccggtcga ttcgcgacga gttctcgttg tcggtgctgc tcgtcgagca
     3481 ccacatggcg atggtgatgg cgatctcgga gaagatcgtc gtgctcgact tcgggtcgaa
     3541 gatcgccgag ggcggcgccg aggagatcag cggcaacccg cgggtcatcg aggcctacct
     3601 cggggcaccg ccggacacgg gaacggcggg atgatcgacg cggggagcgt gctgctccag
     3661 gtcgccgagc tgcgcgcggg ctacgggccc gtgcaggtgc tccacggcgt cgacttcgcg
     3721 gtgcacgagg gtgaggtcgt cgtcattctc ggcgccaacg gcgcgggcaa gacgacgaca
     3781 ctgcgcgcca tctccggcat gatccacgct cgcggacgcg tcgagttcgc gggccagtcg
     3841 ttgctcggcc tcaagcccga acaggtcacg cgcgccggta tcgcgcacgt gccgcagggc
     3901 cgcggcacca tcgccgacct caccgtcgac gagaacctcc gcatcggcgc gtacacacgc
     3961 aaggatcgtg gggtcgacga cgacatcgac cgctggtaca cgatgttccc gcgtctcggc
     4021 gagcggcgta cgcaagccgc ggggagcatg agcggtggtg aacagcagat gctcgcgatc
     4081 tcgcgggcgc tgatgtcacg cccgaagctg ttgctgctcg acgagccatc gctcggtttg
     4141 gcgccgatcg tgacgcaggg actgttccgc gtgctcggcg agctcaaccg cgaccacggc
     4201 acctcgatgc tcatcgtcga gcagaacgcg caactcgcgc tcgggatcgc tcgccgcggc
     4261 tacgtgctcg agaccggatc gatcgcggtc tcgggggaag cgcaggcgtt gagcaccaac
     4321 gacgacgtcc gccgggcgta cttggggatc tgatggacct cttcttcgcg caactcatca
     4381 acggcatcgg cgccggcgtc gtctacgcgt cgctcgcgct ggcgctcgtc ctcatcttcc
     4441 gcacgaccgg catcctgaac ttcgcccaag gcgagatggc gttggtttcg acgtacttga
     4501 cgtggaagtt caccgactac ggctggccgg tgtggctctc gatcgtcgcg agcgtcgcga
     4561 tctcgttcgt cggcggcgcg ctcatcgaac gcatcatcat ccgcccggtc gagcgatcgt
     4621 cgcccttggt gatcgtgatc gtgacgatcg gcttgttcct cgcgctcaac tcgctcgcgc
     4681 aggtgatctt cggcaccgac aacaaggtga tgccgcgctt ctacgagttc cacgtctggg
     4741 agctcgccgg catccgtgtc agctccgaca ccgtggtgtt ggtcgcggtg ctcgctgtcg
     4801 agtgcgcgct gctctggctc ctgctgcagc gcacgaagat cggcctcgcg ctgcgggccg
     4861 tggcgtcgaa cgccgagtcg agccgcctcg tcggtgtatc ggtcggcacg atgctcatgt
     4921 tcggctgggg cctctccgcc gcgctcggtg cgctcgccgg gtcgctcgtc atcccgacga
     4981 cggggtcgca gtcgctcacc gcggggtcga tgcaggcgat cctgttctac gcgttcgcgg
     5041 ctgccgccat cggcggcttc gacagcccgg tcggcgccgt cgtcggcggg atgatcgtcg
     5101 gcgtcaccca ggccctcacc atcgagtacg tcgacgcgct cgacggcatc gagtcggtcg
     5161 tgccgctggc gctgatcctg ctcgtgctgc tgttcaagcc gtcgggcctg ttcggcaagg
     5221 tgcaggtcga acgcgtatga cggtctcgcg cgtcgtcaag ggaattgtgt tcctcgtgct
     5281 gctcggcgcg ctgttgtacg tgccgcagta ctacgaggcg acgaccgtca accatttcac
     5341 gaccgcgatc tacgtcgcga tcgcggccct cggcctcaac atcctgaccg gttacaacgg
     5401 ccaagtgtcg atcgggcacg gtgtcttctt cgcgctcggt agctacacga ccgcgatcct
     5461 catgcgcgac cgcgattggc cgttcctggc gacgatcccc gtcgtgctcg tcgtgtgctt
     5521 cgtcgtcggc ggactcgtcg gctttcccgc cttgcgcgtc aagggcctgt acctcgcgct
     5581 cgtcacgttg ggtgtcgcgg tcgtcttccc cgatctcgcc cgcaagttcg tgaatcgcga
     5641 cggcatctgg gaccccaacg gcgaacccgg cgtcgtgcaa ctgtcgatcg tcaggaacct
     5701 gcgaccaccg aactggtaca cgtggttccc cgactcgatc gccgaccatt tcgacgagtg
     5761 gatccacgac cgcgaccagt gggcgttcta ctgcgcgatc acgctcgggc tcgtgctgtt
     5821 gctcttggtc tggctgatcg tgcgcagccg cttcgggcgc gcgctcatcg cggtccgcga
     5881 tcacgaagcg gccgcggaga ccgtcggcat caacctcgcg cgcgtcaagg tcaccgcgtt
     5941 cgcactcagc gcgctgtacg ccggtgtcgc cgggtcgatc gcagtgttgc tcgaccgcag
     6001 cgccagcgtc gacaaggtcg aggtgttcca ggattccatc ctgttcctcg tcgccgtcgt
     6061 gatcggcggc actgcgaccg tgatcggccc gatcgtgggc gcgttcgtgc tcgtgttctt
     6121 ccaggagtgg gcgaacgagc agtcgaccct gcaggattgg ctcggtgacg aacgcgcgaa
     6181 gctgttctcg cccgcgctgt tcggcatcgc gttgatcgtc tcgatgtact tcttgcccga
     6241 cggaatcgtc ggcggcgggc ggcggttgtg gcggcgcgtg cgccggcgac acccggtcgc
     6301 cgaatctgtc aactagaaga gaccgaaagg aagccaccca tgcgcgttcg atctgcgaag
     6361 ccgttccggc tgctcgcgtt ggccgtcgcc gccgcgaccc tgttcgccgc gtgcggcgac
     6421 ggtcgcgatg acgacgacag ctccagcgac acgaccgcgg cctccggcac accgaccagc
     6481 gcgggtgaag gtggcggcgg cgcggcgacg ttcgacatcg acaccgccga ctgcgagacc
     6541 gatcccgcga gtgtgacgat cgagggcgac acgatcaagc tcggcaccag cctcccgcag
     6601 tcgggcatct acgccgcgtt cgccgagatc ctccgcggcg agcaggcgta catccagtac
     6661 ctcaacgacg acctcggtgg cgtcgaggtc gcgggcaaga cgtacaaggt cgagctcgtc
     6721 gccaaggacg acggctacaa ctccgatcag acggtggcga acgtcgacag cctgatcgcg
     6781 gacgacgacg tgttcgcgct cttcaacgtc gtcggcacga agaacaacct cgcgatccgt
     6841 gacagcgtgc agacgaactg catcccgaac ctgttcgcgg cgaccgggtc acccgcgtgg
     6901 ggcaaccccg agtatccctg gatgctcggc acgtacctcg tgccctaccc gctcgaggcg
     6961 caggcactcg tgaactacct cgaggacaac aaccccacgg cgtcgctcgc gatcctgcgc
     7021 gccgacgacg acttcgggcg cgcgtacgcc gacacgctga aggcgttgat cgagggtacc
     7081 gacatcacga tcaagcagga agagacgtat gaccccgagg gcgccgaggt tgccgcgcag
     7141 gtgacgagtc tcgccgcgag cggcgccgac gtcttcgtgc tcggcgcgac gttgctcgcg
     7201 tgcccgtcgg ccctgaacga gatgggcaag gcgggctgga agccgtcggt cgtctacatg
     7261 tccggcacgt gcacgtcgaa gacgctcatg gccgcggcgg gtgacaacgg tgcgggcgtg
     7321 ctctcggtgt cgccgctcat ggatccgaac gacccgcagt tcgcgagcga cgaggcgatg
     7381 acgctgtaca aggagaagat cgcgcagtac ggcaacggtg cggacgcgac gaacggcatc
     7441 gtcgcctacg gctggagcgc gggcgccgcg ttgcagaagt cgctggaggc tgcgtcggcg
     7501 ccgaaccggt tggcggtcat gcaagcggcg cggacgctcg atctctccga cgtcggcctg
     7561 cagctcccgg gctccgagtg ggtcgtcggc caagacgact ggttcctcgg cgaggacttc
     7621 aacctggtgc agtacgacct cgccggcaag tacttcaagc cggtcggaga cctcatcaag
     7681 gagaacggcg agaccgagaa gatcacgccc gagaacctga tcaacggctg agct
//
LOCUS       DASUGA010000398         7141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_532835, whole genome shotgun sequence.
ACCESSION   DASUGA010000398 DASUGA010000000
VERSION     DASUGA010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532835"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..643
                     /locus_tag="VFX21_07935"
     CDS             236..643
                     /locus_tag="VFX21_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5095926.1"
                     /translation="MELFAGCSPRQCRELQSVCTPMDLATGRVLCRQGEVAEQCFIVV
                     SGHADVFMDGALVAEVNAGELIGELALLGGPRRRNATVIAASDMRVLALSRREFSSLN
                     ATSLTVQQRVLGTAIRRLIENAQRATPALSAPR"
     gene            complement(653..1588)
                     /locus_tag="VFX21_07940"
     CDS             complement(653..1588)
                     /locus_tag="VFX21_07940"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX5095927.1"
                     /translation="MTTLTVQPIREDIGFGARVRGVTQEALRDTAVRAEINDLFERYG
                     MLIFEDIEPTPRMQVALSEVFGPLKDHPSQATARAAGDDLLGVIEMRHDPDDGSVRVD
                     GRALSTWLPWHFDHCYNDELNRAGVLRAVEVPPEGGRTGFVDGIALYNAIPAGVRERI
                     EGETVIYAMDVIMANLRYGRPADFEEIDAGDQARAVMEEFATRPRALHPAVWTRRTGE
                     KVLHVSPWMAKGIVGRDDAEADALLMEVSDAIVEAAQGLCYHHDWKPTDMVIWDNWRC
                     LHSVAGTPPQYARCMHRTTIKGDYGLGRFEEVPVA"
     gene            complement(1591..2454)
                     /locus_tag="VFX21_07945"
     CDS             complement(1591..2454)
                     /locus_tag="VFX21_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5095928.1"
                     /translation="MPVAEIDGIEIAYELVGDGPPWVLTPGGRFTKEVGGLPEMARAL
                     ADTGQRVLTWDRPNCGASSVVFHGRSESEVQAEALGALIRHLDLGPTVIAGGSGGSRI
                     SLLAAARNPDVTAGLAMWWISGGVPGLLQLANYYCMPSADAAWHGGMEAVAALDQWQE
                     VQEKNPSNRARILAMDRREFIETMDRWMVAYCPCDDALVPGLDDEDITRLDVPILVFR
                     SGASDMSHTRATSEAIAKGLPGAELAEPPWGDDEWNERRAEVEQTGSLFVRWPLLVPQ
                     LVEWADRTVRS"
     gene            2539..3414
                     /locus_tag="VFX21_07950"
     CDS             2539..3414
                     /locus_tag="VFX21_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095929.1"
                     /translation="MAMQGRREAAVARVSVVIQAIRDMDEDSVTSAVEGLSHRHRLLA
                     PLALVAGAFVMLLDGLKLVVSNWRLTLVQVLPAMWIWIAMFDIKVHVLRHREFADVTG
                     PKTAAAFVLITAITAACFYLNATFAFAITQRGGPPDIRAAMREAWSHRRVVLAWGVGV
                     GVLLATAAVVVDRWGLRWFALSMSVVVGIMMVCYVAVPSRLIGATKPHISRRDKLAAA
                     AVGGVVAAIVTTPGYLLGRVGLVLLGSQFLRIPGVVFIVVGLTLHAGAEGSVKAVKMS
                     SKLLVTRPPETAESR"
     gene            complement(3422..3943)
                     /locus_tag="VFX21_07955"
     CDS             complement(3422..3943)
                     /locus_tag="VFX21_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEX5095930.1"
                     /translation="MAFGRGKDDEQRPQRRKFQMRQKMMSIGDDYWIEDEQGNKAFRV
                     DGKAMRMRDTWVLEDASGKEVATIREKKLTIRDKIKIELASGREATVKKEMISFRDKY
                     HVDIDGADLTVKGDIADHEYKIERDGDKIAEISKKWFRVRETYGVEVADDRDTALILA
                     VTVAVDAMSGIGK"
     gene            complement(4034..4759)
                     /locus_tag="VFX21_07960"
     CDS             complement(4034..4759)
                     /locus_tag="VFX21_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007461098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HEX5095931.1"
                     /translation="MSSLRKTALAAGLFYIATFVFSIPALGFYKDVLDNDAFARGAGN
                     ETAVLWGGLFEVITALTGIGTAVVLYRVLRRDSQSAAIGFVASRTLEAAMIFVGVISL
                     LSVVTMRQDYGTVGDSSNLLPISHALVAVHDWTFLLGPGVMPAINALCFATVMYRTRL
                     VPRIIPTIGLIGAPLLLASSTATLFGAHEQVSTSAMLMALPIAAWELSVGVYMTVKGF
                     RPQPLAELPVIIESSSREPAYAN"
     gene            complement(4761..4973)
                     /locus_tag="VFX21_07965"
     CDS             complement(4761..4973)
                     /locus_tag="VFX21_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015747642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095932.1"
                     /translation="MQYEIRINGHLGSRWAAWFDGFCVTNEGDGTAVLRGPIVDQAAL
                     HGLLDKLRDLGIPLVSLTPISHEEGN"
     gene            complement(5042..>7141)
                     /locus_tag="VFX21_07970"
     CDS             complement(5042..>7141)
                     /locus_tag="VFX21_07970"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5095933.1"
                     /translation="LLELRAADLRFTADEAATYFNDAMGLALTPADVDALESRTEGWI
                     AALQLAALSMQDRDDVGAFIANFTGDDRFVVDYLAEEVLERQPDDVRRFLLDTSVLAR
                     LSGPLCDAVTQRRGGKATLEALDRANLFLVALDDRRTWYRYHHLFADVLRARLADEHP
                     ELIPALHGRASDWFDAHGDADAAIEHALAGAHFERAAQLIELAAPRLRQARQETTLRR
                     WLEALPDEIFGDRPVLSVALVGARMSTGESTDVEQLLDRAESAPEPIVFDTDEYACLP
                     AQIAVQRAGLALLAGDLAGASAHAERDFALVEPTDHFRLGAASALTGLARWPQGDLEA
                     AGLHYADAVRHFLAIGYLADALGCSITVADIQIAQGRLRDAMRTFEDGLRLVTEHPGL
                     RGAADMHVGMSEVLLARGDLADAARHLATSGDFGDLNGLPQNAYRWRVAMAGLRQAEG
                     DIDRALTLLDEAERVYNTDFSPSVRPIPALQARVHLARGDLDAADEWSRRRGLTVDDE
                     LTYVLEFEHITLARILVARQHPDALSFVDRLRAAADAGSRGGTLIELLVLLALVRHQR
                     GDIAAARDAIADALRRAEPEGYLRVFTAEGAAMNALLDTVALDGTAHEHAQRVLRALR
                     AAAPAIPTRQALVDELSARELDVLRLLRSELSGPDIARALNVSLNTMRTHTKNVYAKL
                     GATNRREAVRRADDLGI"
BASE COUNT         1047 a         2399 c         2532 g         1163 t
ORIGIN      
        1 cggcgcccgc gccgcgggcc tgcacgccga gcgcacggtc ggcgtcgccg cggcccggga
       61 gtgcctcgtg cgcaagggga tcctcccggc cgaataggcg ccgggcgcgc caatcgaagc
      121 gcagttggac gccggtcaaa gtcgcccccg cacactcctg aggtaccgga gttggaggtg
      181 cacggtgacg ggttcgaccc agttgcgtat tccgcggcgg tcttggctcc cggagttgga
      241 gctgttcgca gggtgcagcc cgcgtcagtg ccgcgagctg cagtcggtct gcacgcccat
      301 ggacctggcg acgggtcgcg tgctgtgccg gcaaggcgag gtcgccgagc agtgtttcat
      361 cgtggtctcc ggccacgccg acgtgttcat ggacggtgcg ttggtggccg aagtcaacgc
      421 tggcgagctg atcggtgagc tcgcactgct cggcggccct cggcgtcgca acgccacggt
      481 catcgcggcg agcgacatgc gcgtgctcgc gttgtcgcgg cgcgagttct cgagcctgaa
      541 cgcgacgtcg ctgaccgtgc aacaacgcgt gttggggacc gcgatccggc gcctcatcga
      601 gaacgcgcag cgggcgacgc ccgcgctgag cgcgcctcgg tgagccccgg cctcaggcga
      661 ccggcacctc ttcgaagcga ccgagcccgt agtcgccctt gatcgtcgtg cggtgcatgc
      721 agcgcgcgta ctgcggtggt gtgccggcaa ccgagtgcag acagcgccag ttgtcccaga
      781 tcaccatgtc ggtgggtttc cagtcatggt ggtagcagag accctgcgcg gcttcgacga
      841 tcgcgtctga cacttccatg agaagcgcat cggcctcggc gtcgtcgcgg cccacgatcc
      901 ctttcgccat ccacggcgac acgtgcagca ccttctcacc ggtacggcgc gtccagacgg
      961 cggggtgcag cgcgcgtggg cgggttgcga actcctccat cacggcgcgc gcttggtcac
     1021 cggcatcgat ctcttcgaaa tcggcggggc gtccgtagcg gaggttggcc atgatgacgt
     1081 ccatcgcgta gatgaccgtc tcgccctcga tgcgctcgcg cacaccggcc gggatcgcgt
     1141 tgtacagcgc gatgccgtcg acgaaaccgg tccgcccgcc ttcgggcggc acctcgaccg
     1201 cgcgcagcac cccggcgcgg ttgagctcgt cgttgtagca gtggtcgaag tgccacggca
     1261 gccacgtcga cagcgcccga ccgtcgacgc gcaccgagcc gtcgtcgggg tcgtggcgca
     1321 tctcgatgac gccgagcaag tcgtcaccag ccgcgcgcgc cgtcgcctgg ctggggtgat
     1381 ccttgagcgg tccgaacacc tcgctgagcg cgacctgcat cctcggcgtg ggctcgatgt
     1441 cctcgaagat cagcatgccg taccgctcga acaggtcgtt gatctcggcc cgcaccgcgg
     1501 tgtcgcgcag cgcttcttgt gtgacgcccc gtacgcgcgc tccgaacccg atgtcttctc
     1561 gaatgggttg gacggtcaac gtcgtcatcg tcaactcctc acggtccggt cggcccactc
     1621 gaccagttgc ggaacgagca gcggccaccg cacgaacagc gaaccggtct gctccacttc
     1681 ggcgcgccgt tcgttccact cgtcgtctcc ccagggcggt tcggcgagct ccgcgcccgg
     1741 cagccccttg gcgattgcct ccgacgtcgc gcgcgtgtgc gacatgtcgg acgcgccgct
     1801 gcggaacacg aggatcggca cgtcgagccg ggtgatgtct tcgtcgtcga gccccggtac
     1861 gagggcgtcg tcgcacgggc agtacgcgac catccagcgg tccatcgtct cgatgaactc
     1921 gcggcggtcc atcgcgagga tgcgtgcgcg gttgctcgga ttcttctcct gcacctcttg
     1981 ccattggtcg agtgcggcga cggcctccat accgccgtgc cacgcggcgt cggccgacgg
     2041 catgcagtag tagttcgcga gctgcagcag gccgggcaca ccgccgctga tccaccacat
     2101 cgcgagcccc gcggtgacgt cgggattgcg cgcggccgcg agcagcgaga tgcgggaacc
     2161 acccgagccg ccggcgatga ccgtgggccc gagatccagg tggcggatga gcgcgcccag
     2221 cgcttccgcc tgcacttccg attcggagcg gccgtggaac acgacggacg acgcaccgca
     2281 gttcggccgg tcccacgtga gcacgcgctg tccggtgtcg gcgagcgcgc gtgccatctc
     2341 gggtaggccg ccgacttcct tcgtgaagcg gccgcccggc gtcaggaccc aaggcgggcc
     2401 gtcgcccacc agctcgtagg cgatctcgat cccgtcgatc tcggccaccg gcatgacacg
     2461 aagcctacga ccgatactgg caggtgggaa cttcgtacta cgtgacctag cgtcggccgc
     2521 gttgtgagca ctcgacgcgt ggcgatgcag ggtcggcgcg aagccgcagt ggcacgggtg
     2581 agcgtcgtca tccaagccat tcgcgacatg gacgaggact ccgtgacctc cgcggtcgaa
     2641 ggcctgagcc accggcaccg gctcctcgcg ccgttagcgc tcgtcgccgg cgcgttcgtg
     2701 atgctcctcg acggattgaa gctcgtcgtg tcgaactggc gcctgaccct cgtgcaggtg
     2761 ctcccggcga tgtggatctg gatcgcgatg ttcgacatca aggtccacgt cttgcgacac
     2821 cgagagttcg cggacgtcac cgggccgaag accgcggccg cgttcgtcct catcaccgcg
     2881 atcaccgccg cctgcttcta cctcaatgcg acgttcgcgt tcgcgatcac ccagagaggt
     2941 ggtccgcccg acatccgtgc cgcgatgcgc gaagcgtggt cgcaccgacg cgtggtgctc
     3001 gcgtgggggg tgggtgtcgg cgtgctcttg gcgacggcgg ccgtggtcgt cgaccgttgg
     3061 gggctgcgat ggttcgcgct ctcgatgagc gtcgtcgtcg ggatcatgat ggtgtgctac
     3121 gtcgcggtgc cgtcgcggct catcggcgcc accaagccgc acatctcacg gcgcgacaag
     3181 ctcgcggccg ccgcggtcgg aggggtggtc gccgcgatcg tgacgacgcc gggctatctc
     3241 ctgggccggg tcggcctggt cctgctcgga tcgcagttct tgcgcatccc cggcgtcgtg
     3301 ttcatcgtcg ttgggctcac gctgcacgcc ggcgcggagg gctcggtcaa ggcggtgaag
     3361 atgagctcga agctgctcgt cacgcgaccg cccgagacgg cagagtcacg ttgacgcgaa
     3421 gctatttgcc gataccgctc atggcgtcga ccgcgacggt gacggcgagg atgagcgccg
     3481 tgtcgcgatc gtctgcgact tcgacgccgt aggtctcgcg gacccggaac cacttcttcg
     3541 agatctcggc gatcttgtcg ccgtcgcgtt cgatcttgta ctcgtggtcg gctatgtcgc
     3601 ccttgaccgt gaggtccgcg ccgtcgatgt cgacgtggta cttgtcgcgg aagctgatca
     3661 tctccttctt cacggtggcc tcgcggccgg acgcgagctc gatcttgatc ttgtcgcgga
     3721 tggtcagctt cttctcgcgg atggtcgcga cctctttgcc ggacgcgtcc tccaacaccc
     3781 acgtgtcgcg catgcgcatg gccttgccgt cgaccctgaa ggccttgttg ccctgctcgt
     3841 cttcgatcca gtagtcgtcg ccgatcgaca tcatcttctg acgcatctgg aacttgcgac
     3901 gctgcggtct ctgctcgtca tccttgccgc gcccgaatgc cataccggca gcctacgacg
     3961 ggcccgagcc gcgaacgcgc aagcggccgc cgcgtcgaga cgtggcggcc gctcggagtg
     4021 cgacgcgcgg agctcagttg gcgtatgccg gctcccgcga cgacgactcg atgatcaccg
     4081 gcagctcggc gaggggctgg gggcggaagc ccttgaccgt catgtacacg ccgaccgaga
     4141 gctcccatgc cgcgatcggc agcgccatga gcatcgccga ggtcgacacc tgctcgtggg
     4201 cgccgaacag cgtcgccgtc gacgaggcga gcaacagcgg cgcaccgatg aggccgatgg
     4261 tggggatgat gcggggaacg agacgggtgc ggtacatcac ggtcgcgaag cacagcgcgt
     4321 tgatcgcggg catcacaccc gggccgagca ggaacgtcca atcgtgcacg gcgacgagcg
     4381 cgtgcgagat cgggaggagg ttcgacgagt cgccgaccgt gccgtagtcc tgccgcatgg
     4441 tgacgacgga gagcaggctg atgacgccga cgaagatcat cgccgcttcg agcgtccggg
     4501 atgcgacgaa cccgatcgcc gcggactggc tgtcgcggcg cagcacgcgg tagagcacga
     4561 ccgcagtacc gatgccggtc agcgcggtga tgacttcgaa cagaccgccc cagagcacgg
     4621 cagtctcgtt gccggcgcca cgagcgaacg cgtcgttgtc gagcacgtcc ttgtagaagc
     4681 ccagggccgg gatcgagaag acgaaggtgg cgatgtagaa aaggccggcg gcgagcgcgg
     4741 tcttgcgcag cgatgacatg tcagttccct tcttcgtgac tgatcggggt gagcgagacc
     4801 agcgggatgc cgaggtcgcg cagcttgtcg agcaacccgt gcagcgcggc ctggtcgacg
     4861 atcgggcccc gcaggaccgc ggtgccgtcg ccttcgttgg tgacgcagaa cccgtcgaac
     4921 caggctgccc accgggaccc gaggtgaccg ttgatgcgga tctcgtactg caccgggaga
     4981 ccgtacgaac cgacctggtg agtgcggatc accacatgtg gtgagcgccg ctggtgattg
     5041 gctagatgcc gaggtcgtcg gcccggcgga ctgcttcgcg gcggttggtg gcgccgagct
     5101 tggcgtagac gttcttggtg tgcgtgcgca tcgtgttgag cgacacgttc agcgcacggg
     5161 caatgtcggg tccactcagc tcgctgcgca gcaggcggag cacgtcgagc tcgcgggcgc
     5221 tcagctcgtc gacgagcgcc tgccgggtcg gtatcgccgg cgcggcggct cggagcgcgc
     5281 gcaagacccg ttgcgcgtgc tcgtgcgcgg tgccgtcgag tgcgacggtg tcgagcaggg
     5341 cgttcatcgc ggcgccctcg gcggtgaaga cccgcaggta gccctcgggc tcggcccgcc
     5401 gcagcgcgtc cgcgatcgcg tcgcgtgcgg ctgcgatgtc gccgcgctgg tggcgcacga
     5461 gggcgagcag cacgagcagc tcgatgagcg ttccgccgcg tgaccccgcg tccgcggcgg
     5521 cgcgcaggcg gtcgacgaag gacaacgcat ccgggtgttg gcgcgcgacc aggatgcggg
     5581 cgagcgtgat gtgctcgaac tcgaggacgt acgtgagctc gtcgtcgacc gtgaggccgc
     5641 ggcggcgcga ccactcgtcg gccgcatcga ggtcgccgcg cgcgagatgc acacgcgcct
     5701 gcaacgccgg gatcggacgc accgacggcg agaagtcggt gttgtagacg cgctcggctt
     5761 cgtcgagcag cgtcagcgcg cggtcgatgt cgccttcggc ctggcgcagt ccggccatcg
     5821 cgacccgcca tcggtacgcg ttctgcggca gcccgttgag atcgccgaag tctccgctcg
     5881 ttgccagatg ccgcgcggcg tcggcgaggt caccgcgtgc cagcagcact tcgctcatgc
     5941 cgacgtgcat gtcggccgcg ccgcggagac cgggatgctc ggtgacgagg cgaagtccgt
     6001 cctcgaaggt tcgcatcgcg tcgcgcagac ggccttgcgc aatctggatg tcggcgaccg
     6061 tgatggaaca accgagcgcg tccgcgaggt agccgatggc gaggaagtgc cggaccgcgt
     6121 cggcatagtg caggcccgcg gcctcgaggt cgccctgcgg ccaccgcgcc aggccggtga
     6181 gcgcggacgc ggcaccgagg cgaaagtgat cggtcggctc gacgagcgcg aagtcccgtt
     6241 cggcgtgcgc gctcgcaccc gccaggtcgc ccgcgaggag cgcgagcccg gcgcgctgga
     6301 ctgcgatctg cgcgggcagg cacgcgtact cgtcggtgtc gaacacgatc ggctccggcg
     6361 ccgactcggc gcggtcgaga agctgttcga cgtcggtcga ctcgccggtc gacatgcggg
     6421 cgccgaccag cgcgacgctc agcaccggcc ggtcaccgaa gatctcgtcg ggcaacgctt
     6481 cgagccaacg ccgcagcgtc gtctcctgcc gggcttggcg caggcgcggt gcggccagtt
     6541 cgatcaactg cgcggcccgc tcgaagtgcg cacccgcgag cgcgtgctcg atcgcggcat
     6601 ccgcgtcgcc gtgcgcgtcg aaccagtcac tcgcccggcc gtgcaatgcc gggatcagct
     6661 cggggtgctc gtcggcgagc cgggcgcgca acacgtcggc gaagaggtgg tggtagcggt
     6721 accaggtgcg gcggtcgtcg agcgcgacga ggaacaggtt cgcgcggtcc agtgcctcga
     6781 gcgtcgcctt gccgccgcgg cgttgcgtca ccgcgtcgca cagcggccca ctgagccggg
     6841 cgagcaccga tgtgtcgagc aggaagcgcc gcacgtcgtc cggctggcgt tcgagcacct
     6901 cctccgcgag atagtcgacg acgaaccggt cgtcgcccgt gaagttcgcg atgaacgcgc
     6961 cgacgtcgtc gcggtcttgc atcgagagcg cggcgagttg cagtgcagcg atccagccct
     7021 ccgtgcgcga ctccagcgcg tcgacgtccg cgggtgtgag cgcgagcccc atcgcgtcgt
     7081 tgaagtaggt cgcggcttcg tcggcggtga aacgcaggtc ggcggcgcgc agctcgagca
     7141 g
//
LOCUS       DASUGA010000399         2646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1678468, whole genome shotgun sequence.
ACCESSION   DASUGA010000399 DASUGA010000000
VERSION     DASUGA010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678468"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VFX21_07975"
     CDS             <1..367
                     /locus_tag="VFX21_07975"
                     /inference="COORDINATES: protein motif:HMM:NF037008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095934.1"
                     /translation="IEAFVVPTAELANDTDGGGRDHIGFIAAVLANPEHMPLVIDAFE
                     PTTDRWVAALARVTPNLDADVRELRLALAKDMTNRVLGQPDGPVRAWIAIRAPGADGP
                     LTERLIDFLVGAFAAPQTI"
     gene            381..1274
                     /locus_tag="VFX21_07980"
     CDS             381..1274
                     /locus_tag="VFX21_07980"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX5095935.1"
                     /translation="MAFTSFREWLEALHAQPPFGAGDRAGTANYIDAAARARGVASVV
                     TGTPVSLARKLPPDPAITAGGHPGFTMEVELQHLAPPVAMAVDQVEIHCHGLHSTHVD
                     ALNHLGRNGTWYGDYALDDPDAPSIADLADAGLVTRGVFADVPSVRGTDWADGTVPVN
                     GDDLDAAIAAAGVTFEPGDALLLRMGRDAYEAAGNVYASSVGDPPPPGIGRSGAEWIA
                     DHRASIVGWDFLDSSHASEPPGAVHSLIWAIGLVLIDNCDYSAARAALAEAGTATGML
                     VVAPLRIPGGTGCMVNPLLLI"
     gene            1285..2136
                     /locus_tag="VFX21_07985"
     CDS             1285..2136
                     /locus_tag="VFX21_07985"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5095936.1"
                     /translation="MGTVDYLCNAFVPRYADEWDAAIARQGVPVKVRRDPHDAFCEPD
                     VMVARMDELGIDTLLIVAGDQQDHPGAFDFSALTARFADAEQLAERYPGRFAALWNLD
                     PNRGMTGIDRAVDVLDHPWVVGLYNHVHSWDRPFDHADFYPYYALAADLDVPVAMQAG
                     TSGGLMPSECGRPIGIDRPAIYFRRVRFLLSHTGWPWVDEAVAMALKFPNVFLGTAAY
                     PPKHWAPAVHDFLRGPGRTKTVFGTNFPTVGHRHALAQVAELDLTPETRDALLGGTAR
                     RVFTRLS"
     gene            2133..>2646
                     /locus_tag="VFX21_07990"
     CDS             2133..>2646
                     /locus_tag="VFX21_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017558699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX5095937.1"
                     /translation="MIVPEPDAKARLRTRDIAVPASVVLDADGIGDGVELLHDPLVIK
                     AFGAGIVHKSDIGAVRLGLDADEVPTAIFAMRARLAAEHGIFPDGFLVEEQASGGVEL
                     LVGAVRGPFGVAVTVGLGGVLAEVLDDVAVGLAPLDREGAEALLDGFRGSELLRGYRG
                     AAPVDRAGLVA"
BASE COUNT          358 a          962 c          911 g          415 t
ORIGIN      
        1 catcgaagcg ttcgtcgtgc cgacggccga gctcgcgaac gacaccgacg gcggtggccg
       61 tgaccacatc ggcttcatcg ccgccgtgct cgcgaacccc gagcacatgc cgctcgtgat
      121 cgatgccttc gagccgacca ccgaccgatg ggtcgccgcg ctcgcacgcg tcactccgaa
      181 ccttgatgcc gacgtgcgtg agctgcggct cgcgctggcc aaggacatga ccaaccgtgt
      241 gctcggccag cccgacggcc cggtacgcgc ctggatcgcg atccgggcgc cgggtgccga
      301 cgggcctctg accgagcggc tcatcgactt cctcgtcggc gcgttcgccg cgccgcaaac
      361 gatctgacga ggacggtccg atggcgttca cctccttccg cgagtggctc gaggcgctgc
      421 acgcacagcc gccgttcggc gccggggatc gcgccggcac ggccaactac atcgatgccg
      481 ccgctcgggc gcggggcgtc gccagtgttg tcaccggcac gccggtgagc ctcgctcgca
      541 agctcccgcc cgatcccgcg atcacggctg gcggacaccc gggcttcacg atggaggtgg
      601 agctccagca cctcgcgccg ccggtcgcca tggccgtcga tcaggtggag atccactgcc
      661 acgggttgca cagcacgcac gtcgacgcgc tgaaccacct cggccgcaac ggcacctggt
      721 acggcgacta cgccctcgac gaccccgacg cgccgtcgat cgccgacctc gccgacgccg
      781 gcctcgtgac gcggggcgtg ttcgccgacg tcccctcggt acgcggcacc gactgggccg
      841 acggcaccgt gccggtcaac ggcgacgacc tcgacgccgc gatcgcggcg gccggcgtga
      901 cgttcgagcc cggcgacgcg ctgctcctgc gcatggggcg tgacgcgtac gaagcggccg
      961 gcaacgtgta cgcatcgtcg gtcggcgatc cgccgccccc cggtatcggc cggagcggcg
     1021 cggagtggat cgcggatcac cgcgcgtcga tcgtcggctg ggacttcctc gactcgtcgc
     1081 acgcgtcgga gccgccgggt gccgtgcaca gtctcatctg ggcgatcggt ctcgtgttga
     1141 tcgacaattg cgactacagc gcggctcgcg ccgcgctggc cgaggcggga acggccacgg
     1201 ggatgctcgt cgtcgcaccg ctgcgcattc ccggcgggac cggatgcatg gtcaacccgt
     1261 tgttgctgat ctgatggccg actgatgggc accgtcgatt acctgtgcaa cgcgttcgtg
     1321 ccgcgctacg ccgatgagtg ggatgccgcg attgcgaggc aaggcgttcc cgtgaaggtg
     1381 cggcgcgatc cacacgatgc gttctgcgaa cccgacgtca tggtcgcgcg catggatgag
     1441 ctcggcatcg acacgctgct gatcgtcgcg ggtgatcagc aggatcatcc gggcgccttc
     1501 gacttctcgg cgctcacggc gcgcttcgcc gacgcggagc agctcgccga gcggtatccg
     1561 ggccgcttcg ccgcgctctg gaacctcgac ccgaaccggg gcatgaccgg catcgaccgt
     1621 gccgtcgacg tgctcgacca cccgtgggtc gtcggcctgt acaaccacgt gcactcgtgg
     1681 gaccgcccgt tcgatcacgc ggacttctat ccgtattacg cgctcgccgc ggacctcgac
     1741 gttccggtcg cgatgcaggc gggcacgtcg ggtgggttga tgccgagcga gtgcggccga
     1801 cccatcggta tcgaccgtcc cgcgatctac ttccgccgcg tgcggttcct gctctcgcac
     1861 accgggtggc cgtgggtcga cgaagccgtc gccatggcgc tgaagttccc caacgtgttc
     1921 ctcggtactg cggcttaccc gccgaagcac tgggctccgg ccgtgcacga cttcctgcgc
     1981 ggaccgggtc gcaccaagac cgtcttcggc acgaacttcc caaccgtcgg ccaccgccac
     2041 gcgctcgcgc aggtcgcgga gctcgatctc acacccgaga cccgcgacgc cttgctcggc
     2101 ggcaccgcgc gtcgagtctt cacgcgcctg tcgtgatcgt ccccgaaccg gacgcaaagg
     2161 cgcggctgcg cacgcgcgac atcgcggtgc ccgcgagcgt cgtcctcgac gccgacggga
     2221 tcggcgacgg cgtcgagttg ctgcacgacc cgctcgtgat caaggcgttc ggcgccggga
     2281 tcgtccacaa gagcgacatc ggcgcggtgc ggctcggcct cgatgccgac gaggtcccga
     2341 cggcgatctt cgcgatgcgc gcgcgcctcg ccgccgagca cggaatcttc cccgacgggt
     2401 tcctcgtgga ggagcaggcg tccggcggtg tcgagctgct cgtcggcgcg gtgcgtgggc
     2461 cgttcggcgt ggcggtgacg gtcggactcg gcggcgtgct cgccgaggtc ctcgacgacg
     2521 tcgccgtcgg ccttgcaccg ctcgaccgcg aaggcgccga ggcattgctc gacgggttcc
     2581 gcggctcgga gctgctgcgc ggataccgcg gtgcggctcc ggtcgaccgt gccgggctcg
     2641 ttgcgc
//
LOCUS       DASUGA010000400         2404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1598241, whole genome shotgun sequence.
ACCESSION   DASUGA010000400 DASUGA010000000
VERSION     DASUGA010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598241"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /gene="purM"
                     /locus_tag="VFX21_07995"
     CDS             <1..935
                     /gene="purM"
                     /locus_tag="VFX21_07995"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX5095938.1"
                     /translation="GDLGGFGGLFAVDWKRYDDPLLVSSTDGVGTKSLIARMADRRNT
                     IGIDCVAMSVDDIAAQGAEPLFFLDYISIGKLVPEEIDELVAGVAKGCRDARCALLGG
                     EMSEHPGVMEPGEFDLVGFAVGVVDRANVLPAVVHTGDRIVGFASPGLRCNGYSLARA
                     ALFERAGRDIHDAAWPGSDRTLADELLEPSLIYAPAMLELANKVPVRAFAHITGGGLP
                     GNVNRVLPENCDAVFERGTWEEPRIFEVIQQAGGISDDEMASVFNLGVGMVAVVPRDA
                     AEAAVDAVRLAGHGASIVGEIVDGHGRVHVTPAV"
     gene            1300..1497
                     /locus_tag="VFX21_08000"
     CDS             1300..1497
                     /locus_tag="VFX21_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BldC family transcriptional regulator"
                     /protein_id="HEX5095939.1"
                     /translation="MEPQDDDALLTPSEVAAMFRVNPKTVTRWARAGKISAIRTLGGH
                     RRFRASEIRQFLEQVEDSERI"
     gene            1524..>2404
                     /locus_tag="VFX21_08005"
     CDS             1524..>2404
                     /locus_tag="VFX21_08005"
                     /inference="COORDINATES: protein motif:HMM:NF039218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HEX5095940.1"
                     /translation="MQRRGANELDRAAPGIRRRVVAFAAVLVVLAAGCGSSSKDTAPT
                     EKVLLIGDSIMDQEGGAAALALRQAGVDAEKIAFWGSGLLTADQYDHGKTRLDGPRPG
                     SEDIHWLSKAKELVAKDRPRLVVVSMNHNLHSPYPTTADGREIKDPASPEAHVMIQTQ
                     VRALLEILRKHGAKVAFVTPPPDKDEFKPEDNPIWRSIKPVLDEEHIPIIDIAPAVSH
                     NGARVEALPDCFGREQKIRRINEPHYERFGAGLAGTALADGIAKLLGEKLDNGAPGEH
                     PVALVSTESGEGYWIVQ"
BASE COUNT          431 a          793 c          808 g          372 t
ORIGIN      
        1 tcggtgacct cggcggattc gggggcctgt tcgccgtcga ctggaagcgc tacgacgacc
       61 cgctgctggt ctcgtcgacc gacggtgtcg gaacgaaatc gctcatcgcg cggatggccg
      121 accggcgcaa caccatcggt atcgactgcg tggccatgtc ggtcgacgac attgccgcgc
      181 aaggtgccga accgttgttc ttcctcgact acatctcgat cgggaagctg gtgcccgagg
      241 agatcgacga gctcgtcgcg ggcgtcgcca agggctgtcg cgacgcgcgc tgcgcgctcc
      301 tcggcggaga gatgtcggag cacccgggcg tgatggaacc gggcgagttc gatctcgtcg
      361 ggttcgcggt cggcgtggtc gaccgggcca acgtgttgcc ggccgttgtc cacaccggcg
      421 accgcattgt cgggtttgcg agcccaggct tgcgttgcaa cggctattcg ttggcgcgcg
      481 ccgcgctctt cgaacgcgcc ggccgcgaca tccacgacgc ggcctggccc ggatcagacc
      541 ggacgctcgc tgacgaactg ctcgagccga gcctgatcta cgcgcccgcg atgctcgaac
      601 tcgcgaacaa agtgccggtg cgcgcgttcg cgcacatcac cggcggcggc ctgcccggca
      661 acgtcaaccg ggtgctgccc gagaactgcg acgcggtgtt cgagcgcggc acctgggaag
      721 agccgcgcat cttcgaggtg atccaacaag cgggtggcat ctccgacgac gagatggcat
      781 cggtgttcaa cctcggcgtc gggatggtcg cagtcgtacc gcgcgacgcg gccgaagccg
      841 cggtcgacgc ggtccggctc gcgggccacg gcgcctcgat cgtcggcgag atcgtcgacg
      901 gacacggccg ggtccacgtc accccggcgg tctgacacga tggtctgaaa caggcctgac
      961 cagggacgat tcggccgatt cgtccggcgg acctggggcc ttcgggggcc tttggccaat
     1021 cggtgccggt tgtcctacaa taggccgagc gacctatgcc gcccggaacg ttctctccga
     1081 gagtgttcac gacggtacag atcgtgtgga agccgtgttg ccaatgtgca aggcttgggt
     1141 ggagtagcct aaaggcccga ttccggcatt taggtacacg gatgacaagg acgtcgagaa
     1201 cagacgaacg tggggacgga cgtggggtac gaaatcagaa gttcctcgca cgctccgcgg
     1261 aatacgcaaa ccatgaacct cgacgaccaa ggtggaacca tggaacccca ggacgacgac
     1321 gccctactca ctccctccga agtcgcggca atgttccgcg tcaaccccaa gaccgtgacc
     1381 cgctgggcgc gggccggcaa gatctcggcc attcggacgc ttggcggaca ccgccgtttc
     1441 cgggccagtg aaatccggca gttcctcgag caggtcgagg acagcgaacg tatttagcgc
     1501 gtcaccggcg cgaacctttc gcagtgcagc gccgaggtgc gaacgaactt gaccgcgcgg
     1561 cgccgggcat ccggcgccgc gtcgtcgcgt tcgcggccgt gctcgtcgtg ctcgcggccg
     1621 gatgcgggtc gtcctcgaag gacaccgcgc cgaccgagaa ggtgctgctg atcggcgaca
     1681 gcatcatgga ccaagagggc ggcgcggccg cgctcgcgct tcgccaggcg ggcgtcgacg
     1741 ccgagaagat cgcgttctgg ggatcgggcc tcctgaccgc ggaccagtac gaccacggca
     1801 agacgcggct ggacggtccg cggcccggtt ccgaagacat ccactggctg tcgaaagcca
     1861 aggagcttgt cgccaaagac cggccgcgcc tcgtcgtggt ctcgatgaac cacaacctgc
     1921 actcgcccta tccgaccacc gccgacggcc gcgagatcaa ggatccggcg agccccgaag
     1981 cgcacgtgat gatccagacc caagtgcgcg ccctgctcga gatcctgcgc aagcacggcg
     2041 ccaaggtcgc cttcgtcacc ccgccgcccg acaaggacga gttcaagccc gaagacaacc
     2101 cgatctggcg atcgatcaag ccggtgctcg acgaagagca catcccgatc atcgacattg
     2161 cgcccgcggt ttcacacaac ggcgcccggg tcgaggcgtt gcccgactgt ttcggtcgcg
     2221 aacaaaagat tcgtcgcatc aacgaaccgc actacgaacg gttcggagcg gggctggccg
     2281 gcaccgcgct ggcagacggc atcgcgaagc tgctcggcga gaagctcgac aatggtgcgc
     2341 ccggcgagca tcccgttgcg ctggtctcga ccgagagcgg cgagggctac tggatcgtgc
     2401 agtg
//
LOCUS       DASUGA010000401         2898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_420071, whole genome shotgun sequence.
ACCESSION   DASUGA010000401 DASUGA010000000
VERSION     DASUGA010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420071"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VFX21_08010"
     CDS             <1..603
                     /locus_tag="VFX21_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013733280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX5095941.1"
                     /translation="DSGVWEGREGERNYVSSKLMCWVALDRAVKLAPALGVDPAVWAH
                     ERDSIRDEILTRGWNEDAGAFTGAFDSDHLDASVLLLPIVGFLPASDPRVEATVAAIE
                     RELVENDLVRRWTGAAPEGAFVMCSYWLAQCHALAGRVDRAREIFERVTGFANDVDLL
                     SEMVDPSTQLLIGNFPQTLSHAALINAAATIDQAIETGAA"
     gene            605..1387
                     /locus_tag="VFX21_08015"
     CDS             605..1387
                     /locus_tag="VFX21_08015"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX5095942.1"
                     /translation="MGVLEDRVAIVTGSDSGIGQATAIEFAREGSDVVVNYLHDEAGA
                     KQTKAQVEAQGRRAIVVQADVSREADVEKLFGEAINAFEHVDILMNSAGVDASGVNVM
                     DIDIDDFDRSMRTNLYGPLLCSKHFIRHHRTSGEGRPGRILNVTSVHEEIPRAGAADY
                     DCAKGALRNLTRTLSLELAETGITVNNIAPGMVLTPFNQDAVDDTRVREEQVQSIPMK
                     RAANPSEIGRLAVFLASDDAAYVSGSSYVMDGALMQNQGQGA"
     gene            1424..1954
                     /locus_tag="VFX21_08020"
     CDS             1424..1954
                     /locus_tag="VFX21_08020"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5095943.1"
                     /translation="MGTAASGTRTLRDGSKVRLRPIAPDDDARLVAFHEKLSRETQRL
                     RFFGPHPHLSIAEVQRFVRVDGNRRVAVVAERDDEIVAVARYDLLGDTDEAEAAFVVR
                     DDLQGQGLGTLLLAELATRARAQGVARLVALTLPENRPMQRVLHAFGPNVTSAFVDGV
                     VRFEIALNETQARRTK"
     gene            complement(2055..>2898)
                     /locus_tag="VFX21_08025"
     CDS             complement(2055..>2898)
                     /locus_tag="VFX21_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5095944.1"
                     /translation="AGANAMLDPDAVGAPEPVDSEVAVLTAAARAATSDLDRARAAVD
                     AGLAALDRYSDAWCLGWFAIAALRVEGDAAAAATKAQRLDEIELAHARAQRIADRWQA
                     AIAALHEQAPFSRVCSLAVAAEMARVAGEDAAVPARAAADAANEISLPYLATYFKIRE
                     AEGVLRHGDEPGGTDRLQRARAAARLHGFAGLDRAVATLARNFQLRLGSGRALTENSD
                     ELSERELEVLSLLVDGRSNPEIAEALLISRHTARAHVSNLLRKLGTTSRVEAVAEAHR
                     RGLT"
BASE COUNT          472 a          979 c         1004 g          443 t
ORIGIN      
        1 gactcgggcg tctgggaggg acgcgagggc gagcgcaact acgtgagctc gaagctcatg
       61 tgttgggttg cgctcgaccg cgccgtgaag ctcgcgcccg cgctcggtgt cgatcccgcc
      121 gtctgggcac acgaacgcga cagcatccgc gacgagatcc tgacgcgcgg ctggaacgag
      181 gacgccggcg cgttcaccgg cgccttcgac tccgaccacc tcgacgcaag cgtgttgctg
      241 ttgccgatcg tcggcttcct gccggcctcg gaccctcggg tcgaagcgac cgtcgccgcg
      301 atcgagcgcg agttggtgga gaacgatctc gtgcggcgtt ggacgggagc cgcacccgag
      361 ggtgcattcg tcatgtgctc gtactggctc gcgcagtgcc acgcgcttgc gggccgcgtc
      421 gaccgggcgc gtgagatctt cgagcgcgtg accggcttcg ccaacgacgt cgatctgctc
      481 tcagagatgg tcgacccctc gacgcagctg ctcatcggca acttcccgca gaccctttcg
      541 cacgcggcgc tgatcaacgc ggccgcgacg atcgatcagg ccatcgaaac aggagcagcg
      601 tgacatggga gttctcgaag accgagtcgc gatcgtcacc ggatcggact cgggtatcgg
      661 acaggccacc gcgatcgagt tcgcgcgtga aggcagcgac gtcgtcgtca actatctgca
      721 cgacgaagcc ggcgcgaagc agacgaaggc gcaggtcgaa gcacagggtc gtcgcgccat
      781 cgtcgtgcag gccgacgtca gcagggaagc cgacgtcgag aagctcttcg gcgaggcgat
      841 caacgcgttc gagcacgtcg acatcctcat gaacagtgcc ggcgtcgacg cgtccggtgt
      901 caacgtcatg gacatcgaca tcgacgactt cgatcgcagc atgcggacga acctgtacgg
      961 cccgctgctg tgctcgaagc acttcatccg gcaccatcgc acgtcgggcg aggggcgtcc
     1021 gggtcgcatc ttgaacgtga cctcggtaca cgaggagatc ccgcgtgcgg gcgcggccga
     1081 ctacgactgt gcgaagggtg cgctgcggaa tctcacgcgc acgttgagcc tcgagctggc
     1141 cgagaccggg atcacggtca acaacatcgc gcccggcatg gtgctcacac ccttcaacca
     1201 ggacgccgtc gacgacaccc gcgtgcgcga ggagcaggtg cagagcatcc cgatgaagcg
     1261 ggcggccaac ccctcggaga tcgggcgcct tgccgtcttt ctcgcctccg acgacgcggc
     1321 gtacgtcagc ggttccagct acgtgatgga cggcgccctg atgcagaacc aaggacaagg
     1381 cgcctagtgc tccggacgga gcacgggtgg cagaatgccg cgcatgggca cggcggccag
     1441 cggcacgcga acgctccgcg acggcagcaa ggttcgcttg cgcccgattg cgcccgacga
     1501 cgacgcgcgg ctcgttgcgt tccacgaaaa gctgtcgcgg gagacgcaac ggctgcggtt
     1561 cttcgggccg catccgcacc tctcgatcgc cgaggtgcaa cgattcgtgc gcgtcgacgg
     1621 gaaccgccgt gtcgccgtcg tcgccgaacg cgacgacgag atcgtggccg tggcgcgtta
     1681 cgacctgctc ggcgacaccg acgaagccga agccgcgttc gtcgtgcgcg acgacctgca
     1741 gggccagggc ctcgggacgc tgctgctcgc agaactcgcc acgcgcgccc gggcccaggg
     1801 agtcgcgcgt cttgtcgcgt tgacgttgcc cgagaaccgg ccgatgcagc gcgtgttgca
     1861 cgccttcgga ccgaatgtca catcggcgtt cgtcgacggt gtcgtgcggt tcgagatcgc
     1921 gctgaatgag actcaggcgc ggcgcacgaa gtagatgatc aggatcacca gaagcacgac
     1981 gatgagtgtt ccgactccta tatacatgtg cgaccgatac ccggcgccgc tcgccgccaa
     2041 ccgtgaggcg agccttaggt gaggccgcgg cgatgcgctt ctgcgaccgc ctcgacgcgc
     2101 gacgttgtgc cgagtttccg caacaggttt gaaacgtgcg cgcgggctgt gtgccggctg
     2161 atgagcagcg cctcggcgat ctccgggttg ctgcgtccgt cgacgagcag gctcagcact
     2221 tcgagctcgc gctccgagag ctcgtcgctg ttctcggtca acgcgcgacc ggagccgagc
     2281 cgcaactgga agttgcgggc caaggtcgca accgctcggt cgagaccggc aaagccgtgc
     2341 agccgtgccg cggcgcgtgc tcgttgcaag cgatccgtcc cgcccggctc gtcgccgtgc
     2401 cggagcacgc cttcggcctc acggatcttg aagtacgtcg ccaggtacgg caacgagatc
     2461 tcgttggcgg cgtcggccgc ggcccgggcg ggtactgcgg cgtcctcgcc ggcgacgcgc
     2521 gccatctcgg ccgcgaccgc gagcgagcag acccgcgaga acggcgcctg ttcgtgcagc
     2581 gccgcgattg ccgcctgcca gcggtcggcg atccgctgcg cgcgagcgtg cgcgagctcg
     2641 atctcgtcga ggcgctgggc cttcgtcgcg gcggccgcgg cgtcgccctc gactcgcagc
     2701 gccgcgatcg cgaaccatcc gaggcaccac gcatccgagt agcggtcgag tgcggcgaga
     2761 ccggcatcga ccgccgcccg ggcccgatcg aggtcgcttg tcgccgcgcg tgcggcggcg
     2821 gtgagcacgg cgacttcgga gtcgacgggc tccggcgcgc cgactgcgtc gggatcgagc
     2881 atcgcgttcg cacccgcg
//
LOCUS       DASUGA010000402         2226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_249473, whole genome shotgun sequence.
ACCESSION   DASUGA010000402 DASUGA010000000
VERSION     DASUGA010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249473"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VFX21_08030"
     CDS             complement(<1..352)
                     /locus_tag="VFX21_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095945.1"
                     /translation="MQLLHSILVLASEETPHGLWNPTIIGVLTVICAVGLFCGSTYLL
                     LGTNLGARLGFLVAAAGLTGFMVLLTTLWLTTPGSATGNSSLDPPHGDTASWKVVEVV
                     NTPADSKIETVRELE"
     gene            complement(355..1893)
                     /locus_tag="VFX21_08035"
     CDS             complement(355..1893)
                     /locus_tag="VFX21_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5095946.1"
                     /translation="MRLIVLLGAEIPEWKNDLRHWLIVLNILAVAFLIFFLIKSLLSR
                     RSQVEEGDRSPANLTPFLPDEDLEGRRLERVQGWALIFAAIVAVALPIYWLREPTRQD
                     QSLTYFDENSVERGATLYANSASPAYNAAVSLQCANCHGLEGVGGVAPATVNGERVAW
                     KAPPLNTVMLRFTEQPECAIEQQVATHVCEVTNIITYGRPGTPMQPWGVAGGGPKNAQ
                     AIADLVAYLRSITIKPEAARAEAETALKQARADDGTCPEYVTCPNIESANAKKALDDA
                     TKARDDAVKATQDALKRPTATPAELDVACKDVETQVEAATPPVTGQLRDEAVACGKLL
                     AANTAFDNRTKSYEWSLEWIRRRANVEDGQILFETYCARCHTEGWSVFDPSAPPGDVD
                     SVDILGLSGGGGGTGGGIGFNLRDGGTTRRFGTDEQGGWALQQQFITEGSDPNKAYGI
                     GGIGSGKMPGFGQMLTPAMIGQIVSYERYCLNTTNYDGVSPPCPTPAKAPVVPSTTTT
                     TAGG"
     gene            complement(1884..>2226)
                     /locus_tag="VFX21_08040"
     CDS             complement(1884..>2226)
                     /locus_tag="VFX21_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5095947.1"
                     /translation="YPKEDLPNAEKVYKPIEIQAMEQGIVALYQKCVHLGCRVPFCES
                     SQWFECPCHGSKYNRVGEKTAGPAPRGLDHWPALIEGETVTIDTGGVPIPGVPIGTDT
                     TGQTAEGPLCV"
BASE COUNT          359 a          722 c          742 g          403 t
ORIGIN      
        1 tctcgagttc gcgcacggtt tcgatcttcg aatcggccgg cgtattgacg acttcgacga
       61 ccttccagct cgcggtatcg ccgtggggcg ggtcgaggct gctgttcccg gtcgcgctac
      121 ccggcgtcgt gagccacaag gtggtcagca gcaccatgaa gccggtgagg ccggcggccg
      181 ccacgaggaa gccgagccgc gcgccgaggt tcgttccgag cagcaggtag gtcgagccgc
      241 agaacagccc gaccgcgcag atcacggtga ggacaccgat gatcgtcggg ttccagagcc
      301 cgtgcggcgt ctcttccgac gcaagcacca ggatgctgtg cagcagttgc acggtcagcc
      361 tccggccgtc gtggtcgtcg tcgagggaac caccggcgct ttggccggcg tgggacacgg
      421 cggcgaaaca ccgtcgtagt tcgtcgtgtt caagcagtaa cgctcgtacg aaacgatctg
      481 gccgatcatc gcgggcgtca gcatctggcc gaagccgggc atcttgccag agccgatgcc
      541 gccgatgccg taggccttgt tcggatcgga accttcggtg atgaactgct gctgtagcgc
      601 ccagccgcct tgctcgtcgg tgccgaagcg ccgggtagtg ccgccgtcgc gcaggttgaa
      661 gccgatgccg ccgccggtac caccgccgcc gccggacaaa ccgagaatgt cgacactgtc
      721 gacgtcaccg ggcggagccg acggatcgaa cactgaccaa ccctcggtgt gacatcgcgc
      781 gcagtacgtc tcgaagagaa tctgcccgtc ttcgacgttc gctcgccgtc gaatccattc
      841 cagcgaccac tcgtaactct tggtgcggtt gtcgaacgcg gtgttcgcgg cgagcagctt
      901 gccgcaggcc acggcttcgt cgcgcagctg cccagtcacc ggcggcgtcg ccgcttccac
      961 ctgcgtttcg acgtccttac aggccacatc gagttcggcg ggggtcgcgg tgggacgttt
     1021 gagcgcgtcc tgcgtggcct tcaccgcgtc gtcgcgcgct ttcgtcgcgt cgtcgagcgc
     1081 cttctttgcg ttggccgact cgatgttggg acaggtgacg tactcgggac aggtgccgtc
     1141 atcagcccgc gcctgcttca acgccgtttc ggcttctgcc ctcgctgctt cgggcttgat
     1201 ggtgatggaa cggaggtatg cgacgaggtc ggcgatcgcc tgcgcgttct tggggccgcc
     1261 accggcgaca ccccacggct gcatcggcgt gccgggccgg ccgtaggtga tgatgttcgt
     1321 gacctcgcac acgtgcgttg cgacctgctg ctcgatggcg cactcgggct gctcggtgaa
     1381 gcgcagcatc accgtgttga gcggcggcgc cttccacgcg acgcgctcac cgttgaccgt
     1441 cgccggcgcg acaccgccga cgccttcgag gccgtggcag ttcgcgcact gcagcgacac
     1501 cgcggcgttg tacgccgggc tcgccgagtt ggcgtaaagg gtcgcgccac gctccaccga
     1561 gttctcgtcg aaataggtga gcgactggtc ctggcgggtg ggttcacgca gccagtagat
     1621 cggcaacgcg accgcgacga tggcagcgaa gatgagcgcc catccctgca cgcgttcgag
     1681 gcggcgacct tcgagatcct cgtcgggcag gaacggcgtg aggttcgcgg gcgaccggtc
     1741 gccttcttca acctggctgc ggcgcgacag caacgacttg atcaagaaga agatcaagaa
     1801 cgcaaccgcg agaatgttca gaacgatgag ccagtgacgg agatcgttct tccattcggg
     1861 gatctcggcc ccgagcagga cgattagacg cacagaggac cctcggcagt ctggccggtc
     1921 gtgtcggtac cgatcggcac gccgggaatc ggcacaccgc cggtgtcgat ggtgacggtt
     1981 tcaccttcga tgagcgcggg ccagtggtcg agaccacgcg gcgcggggcc ggccgtcttc
     2041 tcaccgaccc ggttgtactt cgaaccgtgg cacgggcatt cgaaccactg cgacgactcg
     2101 cagaacggca cgcggcaacc gaggtgcacg cacttctggt acagcgcgac gatgccttgc
     2161 tccatcgcct ggatctcgat cggcttgtac accttctcgg cgttcggaag gtcttccttc
     2221 ggatag
//
LOCUS       DASUGA010000403         2232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1278747, whole genome shotgun sequence.
ACCESSION   DASUGA010000403 DASUGA010000000
VERSION     DASUGA010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278747"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VFX21_08045"
     CDS             complement(<1..257)
                     /locus_tag="VFX21_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5095948.1"
                     /translation="MRAFVSGAAGFIGSHLCEALLARGDDVVGVDCFTPYYDRAAKEA
                     NRRAVPPHRFTFVEADLRSADLHALLDGVDVVFHQAGQPGVR"
     gene            complement(330..1688)
                     /locus_tag="VFX21_08050"
     CDS             complement(330..1688)
                     /locus_tag="VFX21_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5095949.1"
                     /translation="MTVRLVIIGGGPAGNTAATVAASLGAEVTLIEREVVGGAAHLRD
                     CIPSKAMIATGNELTELGHAQTMGLDAHGSLDVAALRTRVATIEQRLCNDITGLLESQ
                     GVRMIHGTGRMTDPHTVVAESADGTEEVTADAILLSTGSRPRIPDWAHIDRERVLTTR
                     DAYPPPEIPEHLIVIGSGVTGVEFTHMFSALGAQVTLIVSRQQVLPYKDPEVAAVLEA
                     EFLRRGVKLLKGARAISIGREGDKVFVACDDGRAADGSHAVFAIGFLPNSEGLGCEAA
                     GVDVNEGGFVPGNRHCQTNVSHIYSAGDLSGRLPLSSVAAMQGRKIAEHVMGLTAREH
                     RHLDYDKAASAIFTDPEIADVGVAEADAFAAGRKLRVTKVPFSANPKSLINGDTRGFV
                     KILSDPATGIVLGGSIVGRNAAELISVVAVAVTNNLKVADIVDSLLVHPALAESLAEA
                     AY"
     gene            1797..>2232
                     /locus_tag="VFX21_08055"
     CDS             1797..>2232
                     /locus_tag="VFX21_08055"
                     /inference="COORDINATES: protein motif:HMM:NF013269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEX5095950.1"
                     /translation="MAELREDSITGNQVIVAPARGTRPHTVGDGASAPAKSAGDCPFC
                     ERHEHETPPEVARTGDGAADTPGWRVRVVPNKYPIVGDGVDGAHEVVILSPDHNGTLA
                     RLAADRAREVMRVLRDRAAHHLDRGCAYVSVFVNQGKAAGASI"
BASE COUNT          347 a          774 c          774 g          337 t
ORIGIN      
        1 cgcacccccg gctgcccggc ttggtggaac accacgtcga cgccgtcgag cagcgcatgg
       61 aggtcggcgg agcgcaggtc ggcctcgacg aacgtgaacc ggtgcggcgg cacggcccgc
      121 cggttcgcct ccttcgccgc ccggtcgtag tagggcgtga agcagtcgac gccgacgacg
      181 tcgtcacccc gcgccaagag tgcctcgcac aggtggctgc cgatgaagcc ggccgcgccg
      241 gacacgaatg ctctcaccgg cccattttcg cacttgcccg aacagacctt cgacacgacc
      301 cgtatgcccg gcttacgggc ttcgtacggc tagtacgcag cttcggcgag cgactccgcc
      361 aacgcgggat gcacgagcaa cgagtcgacg atgtcggcga ccttcaggtt gttcgtgacc
      421 gcgacggcaa caacggagat gagctcggcg gcgttccggc cgacgatcga accgcccagg
      481 acgatgccgg tcgcagggtc ggagagaatc ttcacgaagc cgcgtgtgtc gccgttgatg
      541 agcgacttcg ggttggcgga gaacggcacc ttggtgacgc ggagcttgcg ccccgccgcg
      601 aacgcatcgg cctccgcgac accgacatcg gcgatctccg gatcggtgaa gatcgccgac
      661 gcggccttgt cgtagtcgag gtggcggtgc tcgcgagcgg tgaggcccat cacgtgctcg
      721 gcgatcttgc ggccctgcat cgcggccacc gacgacaacg gcaagcgtcc cgacaggtca
      781 cccgcggagt agatgtgcga cacgttggtc tggcagtgcc ggttgccggg cacgaagccg
      841 ccctcgttca cgtcgacacc ggccgcctcg cagccgaggc cttcgctgtt gggaaggaag
      901 cctatggcga acaccgcgtg gctgccgtcg gccgcgcggc cgtcgtcgca cgcgacgaac
      961 accttgtcgc cttcgcgtcc gatcgagatc gcccgcgctc ccttgaggag cttcaccccc
     1021 cgccgcaaga actcggcttc gagcacggcc gcgacttcgg ggtccttgta gggaagcact
     1081 tgctggcgcg agacaatgag cgtcacctgc gcacccagcg cgctgaacat gtgcgtgaac
     1141 tcgacgccgg tcacaccgga tccgatgacg atgagatgct cgggtatctc cggcggcggg
     1201 tacgcatcgc gcgtggtgag cacgcgttcc ctgtcgatgt gcgcccagtc ggggatacgc
     1261 ggccgcgagc cggtcgagag gaggatcgcg tcggccgtga cctcctcggt gccgtccgcg
     1321 ctctccgcga cgaccgtgtg cgggtctgtc atccggccgg tgccgtgaat catccgcacg
     1381 ccttgcgact cgagcaggcc ggtgatgtcg ttgcacagcc gctgctcgat cgtcgcgacg
     1441 cgtgtgcgga gcgcggcgac gtcgagactg ccgtgcgcgt cgagccccat cgtctgcgcg
     1501 tggccgagct cggtgagctc gttgccggtc gcgatcatcg ccttgctcgg gatgcagtcg
     1561 cgcaggtgcg cggcgccgcc cacgacctcg cgctcgatca gcgtcacctc ggcgccgagc
     1621 gaggcggcga cggtcgccgc ggtgttgccg gccgggccac caccgatgat cacgagtcga
     1681 acggtcacgg ctccaggcta gaggggtggg aatgcggacc ggagcctctc gccgaatgcc
     1741 gcgatgcgca ggaagggtgg ggaggtcgtg ttccgcgatg tctagcctga ccacccgtgg
     1801 ccgaactgcg cgaggactcc atcacgggca accaagtgat cgtcgcgccg gcccggggta
     1861 ctcggccaca cactgtcggc gacggcgcat ccgcgcccgc gaagagcgcc ggcgactgcc
     1921 cgttctgcga acggcacgag cacgagacgc cacccgaagt cgcgcgtacc ggcgacggcg
     1981 cggccgacac accgggctgg cgcgtgcgcg tcgtgccgaa caagtaccca atcgtcggcg
     2041 acggcgtcga cggcgcacac gaagtcgtca tcttgtcccc ggatcacaac ggcaccctcg
     2101 cgcgtctcgc agccgaccgg gcgcgcgagg tcatgcgtgt gttgcgcgac cgggccgcgc
     2161 accatctcga ccgcggctgc gcctacgtgt cggtcttcgt caaccagggc aaggccgcgg
     2221 gcgcgtcgat cg
//
LOCUS       DASUGA010000404         2880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1740511, whole genome shotgun sequence.
ACCESSION   DASUGA010000404 DASUGA010000000
VERSION     DASUGA010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740511"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1644)
                     /locus_tag="VFX21_08060"
     CDS             complement(<1..1644)
                     /locus_tag="VFX21_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX5095951.1"
                     /translation="MGVFNKLLHAGEGKRLKAVQSIVPLVSNLEPEVERLSDEELIAR
                     TGEFKGRVERATADAKNPEHELELVHDVLDDLLPEAFATVREAAKRVLGQRHYDVQVM
                     GGAALHFGWVAEMKTGEGKTLVATLPSYLNALAGRGVHLVTVNDYLARRDAEWMGRVH
                     KFLGMNVGTIIPGDYSPDYKREQYACDITYGTNNEFGFDYLRDNMVVRREQQTQTGAR
                     APHIKPHFFAIVDEIDSILIDEARTPLIISGRATDAAELYYQFARIVRGLQRDRDYEV
                     DEAKRTVVPTEDGIGRVEEALGVENLYEHVNQNFVHQLQQALRGKELFKKDVDYIVSG
                     GEVKIVDEFTGRILEGRRWSEGLHQAVEAKEGVKIKEENQTLATVTLQNYFRMYDKLS
                     GMTGTANTEAGEFAHTYDLQVVSVPTNRPLVRIDNADYIYKGELGKYESAVAEIAERH
                     EKGQPVLVGTISVEKSEVVSRLLGQHGIPHEVLNAKQHEREAEIITQAGQLGAVTVAT
                     NMAGRGVDILLGGNPEGLARRECIREGLVVDSDEYQARYD"
     gene            complement(2036..2713)
                     /locus_tag="VFX21_08065"
     CDS             complement(2036..2713)
                     /locus_tag="VFX21_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014142767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5095952.1"
                     /translation="MSEQRVDELIRVLIADDHALFRRGLELVLGGEPDIEVVGEAEDG
                     IAAVERAGDLNPDVVLLDVRMPGATGIEAARRIRSAMPNVKILMLTVSEEEDDLFASI
                     RAGANAYLLKEVAIEEVPDAVRAVFRGNSLVSPSMASKLFAEFNVLSRRVEDQHGNAP
                     RLTDRELEVLRLVAKGMSNREIAAELVIAENTVKNHIRNILEKLQLRSRMEAAMYAVR
                     EKLVDAT"
     gene            complement(2749..>2880)
                     /locus_tag="VFX21_08070"
     CDS             complement(2749..>2880)
                     /locus_tag="VFX21_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020155044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54 modulation/S30EA ribosomal C-terminal
                     domain-containing protein"
                     /protein_id="HEX5095953.1"
                     /translation="EEAALQMELLGHDFFVFTSVETGRAAVLYRRRDGDLGLIETSG"
BASE COUNT          435 a          949 c          962 g          534 t
ORIGIN      
        1 gtcgtagcgc gcctggtact cgtcgctgtc gacgacgagc ccctcgcgga tgcactcgcg
       61 ccgggcgagg ccctcggggt tgccgccgag gaggatgtcg acgccgcgcc cggccatgtt
      121 ggtggcgacg gtgaccgcgc cgagctggcc ggcctgcgtg atgatctcgg cttcccgctc
      181 gtgctgcttg gcgttgagca cttcgtgcgg aatgccgtgc tggcccaaca ggcgcgagac
      241 cacctctgac ttctcgaccg agatggtgcc gacgagaacg ggctggcctt tctcgtgacg
      301 ctccgcgatc tcggcgaccg cggactcgta cttcccgagc tcgcccttgt agatgtagtc
      361 ggcgttgtcg atgcgtacca gcgggcggtt cgtcggcacc gacacgacct gcaagtcgta
      421 ggtgtgcgcg aactcaccgg cttcggtgtt cgcggtgccg gtcatcccac tgagcttgtc
      481 gtacatgcgg aagtagttct gcagcgtgac ggtcgcgagg gtttggttct cctctttgat
      541 cttcacgccc tctttggctt cgaccgcttg gtgtagtcct tcgctccaac gccggccctc
      601 gaggatgcgg cccgtgaact cgtcgacgat cttcacttcg ccgcccgaga cgatgtagtc
      661 gacgtccttc ttgaacagtt ccttgccgcg cagcgcctgt tgcagctggt gcacgaagtt
      721 ctggttcacg tgctcgtaga ggttctcgac cccgagcgct tcctcgacgc gcccgatgcc
      781 gtcttcggtc gggacgacgg tgcgcttggc ctcgtcgacc tcgtagtcgc ggtcgcgttg
      841 caggccccgc acgatgcggg cgaattggta gtagagctcg gcggcatcgg tggcacgacc
      901 ggagatgatg agtggcgtac gggcttcgtc gatgaggatc gagtcgatct cgtcgacgat
      961 cgcgaagaag tgcggcttga tgtgcggagc gcgcgcaccg gtctgcgtct gctgctcgcg
     1021 ccgcacgacc atgttgtcgc gcaggtagtc gaagccgaac tcgttgttcg tgccgtacgt
     1081 gatgtcgcac gcgtactgct cgcgcttgta gtcgggcgag tagtcgccgg ggatgatcgt
     1141 gccgacgttc atcccgagga acttgtggac gcgtcccatc cattcggcgt cgcggcgggc
     1201 gaggtagtcg ttgaccgtga cgaggtgcac accgcgcccg gcgagcgcgt tcaggtacga
     1261 aggcagcgtc gcgacgagcg tcttgccctc gccggtcttc atctccgcga cccaaccgaa
     1321 gtggagcgcg gcgccgccca tgacctgcac gtcgtagtga cgctggccga gcacccgctt
     1381 ggcggcctcg cggacggtgg caaaggcctc gggcaggagg tcgtcgagca cgtcgtggac
     1441 gagctccagc tcgtgctcgg ggttcttggc gtcggccgtc gcccgctcga cgcggccctt
     1501 gaactcgccc gtgcgcgcga tcagctcctc gtcggagagc cgttcgacct cgggctcgag
     1561 gttggagacc agcgggacga tggactggac cgccttgagg cgcttgccct cgcccgcatg
     1621 gagaagcttg ttgaacacgc ccatgcggct ggtgatgcta ccggcgcccg gagggcctcc
     1681 cgggccgccc ggcgagggcc acaaacatgg ctccctccgc tccgagccga gcggcctgcg
     1741 gcgagaaggc ctccgctgcg tccgcacggt ccgagcgaaa tgagtagtcg ctactcacgc
     1801 cgccggacgg ccttccccga tggtgggtgc acccaatcgg cccacccacg gaggagcaca
     1861 taccaatggg tgcgccccgg gtggcgccgg ccgagcgcga gccgagtctc tgatgggctt
     1921 ctgaagagac ctcaacaagc cgaacaagca ggcgaaggcg atcgacaaga acgcgaccgc
     1981 gggcgagacg gccaccgcgc aagtgccggc gacggtgctc gtctagccga cggtgttacg
     2041 tcgcgtcgac gagcttctcg cggactgcgt acatcgcggc ttccatgcgc gagcgcagtt
     2101 ggagcttctc caagatgttg cggatgtggt tcttcacggt gttctccgcg atgaccagtt
     2161 ccgcggcgat ctcgcggttc gacatgccct tcgcaacgag gcggaggact tcgagctcgc
     2221 ggtcggtgag acgcggcgcg ttgccgtgct gatcctcgac ccgtcgcgac agcacgttga
     2281 attcggcgaa cagcttcgac gccatcgacg gtgacacgag gctgttgccg cggaacacgg
     2341 cgcgcaccgc gtcgggaacc tcttcgatcg cgacttcctt gagcagatac gcgttcgcgc
     2401 cggcgcggat cgatgcgaac aagtcgtctt cttcttcgga gacggtcagc atcagaatct
     2461 tgacgttcgg catcgcgctg cggatacggc gagccgcttc gataccggtc gcgcccggca
     2521 tccggacgtc gagcaagacg acgtcggggt tcaggtcgcc ggcccgctcg accgcggcga
     2581 tgccgtcctc ggcctcaccg accacctcga tgtcgggttc gccgcccagc acgagctcca
     2641 gaccgcgccg gaacagcgcg tgatcgtccg cgatcaagac ccgtatcagt tcgtcgactc
     2701 gctgttcact cacagagttt gtccctccgg cccgcccgcg cttcgtcgtc agccggaggt
     2761 ctcgatgagg ccgagatcgc cgtcacgccg ccggtagagc acggcagcgc gcccggtctc
     2821 gacactggtg aagacgaaga agtcatgccc caacagctcc atctggagcg cggcctcttc
//
LOCUS       DASUGA010000405         2716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_34942, whole genome shotgun sequence.
ACCESSION   DASUGA010000405 DASUGA010000000
VERSION     DASUGA010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34942"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="VFX21_08075"
     CDS             complement(<1..1123)
                     /locus_tag="VFX21_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX5095954.1"
                     /translation="MANDLEARAINVIRGLSMDAVQKANSGHPGTPMALAPLAHVLFT
                     RVMKYDASDGDWIDRDRFVLSAGHASMLLYSMLYLCGLGLELDDLRDFRQFGSKTPGH
                     PEKGHTKAVEVTTGPLGQGTGNAVGIAVAEAHLRARFGTDLVDHHTFAIASDGDLMEG
                     ISHEAASFAGHFKLGNLVMVYDDNHITIDGPTELTYTDNVPERYRAYGWHVVELGEVA
                     EDLDALEGGLHMGIAETERPTLLVLRSHIGYPSPKVQDTAAAHGNPLGADEVKVVKGI
                     LGLPEDQDFYVPADVLAYYREAGLRGKPAREAWDKRKAAMDPALAEEFDACIAGRGVA
                     GWEQKLPSWEAGASLATRVACADVLTSVFDVVPGLMGGAG"
     gene            complement(1184..2338)
                     /locus_tag="VFX21_08080"
     CDS             complement(1184..2338)
                     /locus_tag="VFX21_08080"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX5095955.1"
                     /translation="MDLVNRYAAAMSLHPTPVEAVGEVAGEILERFDGERPDLLVCFA
                     SPHHVGAFEDVASGLGKLLEPEIMVGGSAVAIAGGATEVESSPALSIFAARFAGGRLD
                     PLTLDALQTPDGHVINGWPDDVPDHGTLLMFADPYSFPVPDFLRLCNAQVPNLTVMGG
                     LASSASGPGGNRLMNGERVVTSGAVAVLLSEDVPVTPVVSQGCRPIGQPFTVTNAQQN
                     LVLELAGQPAMHRLQELVLAADEDERELMRQGLHLGVVVDEHKLDFSRGDFLVRNVLG
                     ADRNNGAIAVGDLVQVGQTVQFHVRDANAADEDLRARLTSVEGDAALLFTCNGRGSHL
                     FGAPNHDAGVVDDLLGPLPLAGAFCAGEIGPVGGKNFLHGFTASLAVFGR"
     gene            complement(2411..>2716)
                     /locus_tag="VFX21_08085"
     CDS             complement(2411..>2716)
                     /locus_tag="VFX21_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095956.1"
                     /translation="TGAFFSTAVESYPTTLEPHLVVLVRSPQNTDGNGTLEVVFMRDE
                     EEVGRNAQSFFVEPGKFGYRLVKGELEFPGPGTVEARCRIVESGAAITVPLTALPVS"
BASE COUNT          425 a          947 c          933 g          411 t
ORIGIN      
        1 cgcccgcgcc gcccatcagc ccgggcacga cgtcgaagac cgacgtgagc acgtcggcgc
       61 acgcgacacg cgtcgccaac gacgcgcccg cctcccacga cggcagcttc tgctcccagc
      121 ccgcgacgcc gcggcccgcg atgcacgcgt cgaactcctc cgcgagcgcg gggtccatcg
      181 cggccttgcg cttgtcccat gcctcgcgcg cgggcttccc gcgcaggccg gcctcgcggt
      241 agtacgcgag gacgtcggcg gggacgtaga agtcctggtc ttcgggcagg ccgaggatgc
      301 ccttcacgac cttgacctcg tccgcgccga gcgggttgcc gtgtgcggcc gcggtgtcct
      361 ggaccttggg cgacgggtag ccgatgtgcg agcggagtac gagcaacgtg ggccgttcgg
      421 tctcggcgat gcccatgtgc agcccgccct cgagcgcgtc gaggtcttcg gcgacttcac
      481 cgagctcgac gacgtgccag ccgtacgcgc ggtatcgctc ggggacgttg tcggtgtacg
      541 tgagctcggt cgggccgtcg atcgtgatgt ggttgtcgtc gtagaccatc acgaggttgc
      601 cgagcttgaa gtgaccggcg aacgacgcgg cttcgtgact gatgccttcc atcaagtcgc
      661 cgtcgctcgc gatcgcgaag gtgtggtggt cgaccaagtc ggtaccgaag cgggcgcgca
      721 agtgcgcctc ggcgacggcg atgccgaccg cgttgccggt gccctggccg agcggaccgg
      781 tcgtcacctc gaccgccttg gtgtgcccct tctcggggtg gcccggtgtc ttcgaaccga
      841 actggcggaa gtcgcgcagg tcgtcgagct ccaacccgag cccgcacagg tacagcatcg
      901 agtacaggag catcgacgcg tggccggccg acagcacgaa ccggtcgcgg tcgatccagt
      961 cgccgtcgga cgcgtcgtac ttcatcacgc gtgtgaacag cacatgcgcg agcggcgcca
     1021 gtgccatcgg tgtgcccggg tgacccgagt tcgctttctg gacggcgtcc atcgaaagcc
     1081 ctcggatgac gttgatcgcg cgtgcttcga gatcgttggc cacgaaactc ctcctccagc
     1141 cctggggcga gactaggcgg agcgggccct cgaaccagcg accttagcga ccgaaaacgg
     1201 cgaggctggc cgtgaacccg tgcaggaaat tcttcccgcc gacggggccg atctcaccgg
     1261 cacaaaaggc accagcgagg gggagcgggc cgaggaggtc gtcgaccaca cccgcgtcgt
     1321 ggttcggcgc gccgaacaga tgtgagcccc gcccgttgca cgtgaagagc agcgcggcgt
     1381 cgccctcgac cgaggtgagg cgcgcgcgca ggtcttcgtc ggccgcgttc gcatcccgca
     1441 cgtggaactg cacggtctgt ccgacctgca cgaggtcgcc gaccgcgatc gcgccgttgt
     1501 tgcggtccgc gccgaggaca ttgcgcacga ggaagtcgcc gcggctgaag tcgagcttgt
     1561 gctcgtcgac cacgacgccg aggtgcagcc cttggcgcat gagctcgcgc tcgtcctcgt
     1621 cggccgccag caccagctcc tgcagccggt gcatcgcggg ttggcccgcg agctccagca
     1681 cgaggttctg ttgcgcgttc gtgaccgtga acggttgacc gatcggccgg cagccctgcg
     1741 agacgaccgg cgtcaccggc acgtcctcgg acaacaacac ggccaccgca ccggatgtga
     1801 cgacgcgctc gccgttcatc agccggttgc caccgggacc cgacgccgac gacgcgagcc
     1861 cgcccatcac cgtgaggttc ggcacctgcg cgttgcacag gcgcaggaag tcgggcaccg
     1921 ggaacgagta cgggtcggcg aacatcaaca gcgtgccgtg gtcgggcacg tcgtccggcc
     1981 agccgttgat cacgtggccg tcgggggtct gcagcgcgtc gagcgtaagc gggtcgagcc
     2041 gcccgccggc gaagcgcgcc gcgaagatcg acagggccgg cgagctctcg acttcggtgg
     2101 cgccacctgc gatcgccacc gccgaaccac cgaccatgat ctccggttcg agcaacttgc
     2161 cgagccccga tgccacgtct tcgaatgcgc cgacgtggtg cggcgacgcg aagcacacga
     2221 gcaggtcggg gcgctcgccg tcgaagcgct ccaggatctc gcccgcgact tcaccaacgg
     2281 cctcgaccgg cgtgggatgc agcgacatcg cagccgcgta ccggttcacg agatccatgc
     2341 ctcccgggat tcggcgcgca gatggcgggg gttgagggcg atcacgtcac accctcccgg
     2401 gtcgcggtcg tcacgaaacc ggaagcgccg tgagcggcac ggtgatcgcg gcgccgctct
     2461 cgacgatccg gcaccgggcc tcgacagtgc ccggcccggg gaactccaac tcgcccttca
     2521 ccaaccggta gccgaacttg ccgggctcga cgaagaagct ctgggcgttg cgccccacct
     2581 cttcctcgtc gcgcatgaac acgacttcca gggtgccgtt cccgtcggtg ttttgcggcg
     2641 agcgcacgag caccacgagg tgcggctcga gcgtcgtcgg gtagctctcg accgcggtcg
     2701 aaaagaacgc accggt
//
LOCUS       DASUGA010000406         3517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_309899, whole genome shotgun sequence.
ACCESSION   DASUGA010000406 DASUGA010000000
VERSION     DASUGA010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309899"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..3285)
                     /locus_tag="VFX21_08090"
     CDS             complement(85..3285)
                     /locus_tag="VFX21_08090"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX5095957.1"
                     /translation="MRNRRCAFGALVGATLLLATMSLPLVTEQAAASTLPDGFTDTVV
                     WSGRTLPTNLAFAPNGKVFLAEKSGQIWVYDNTADTSATLFKDLSTNVFNFWDRGLLG
                     LTVDPRLGNGTGHDYIYVSYAKDAPPGQSTPYWGDDCPTPPEGDTDGCVVLGRLSRIP
                     VNANGTAGAEQVMIDNEWCQQFTTHSLGDLHFGADGMLYVTGGEGAGYASADWGQFGG
                     SLPGTPTPVNPCGDPPGAVGVTNTSPTGRGGALRAQSVRRPPGEPRLLSGTLLRINPD
                     TTNAEGAPGNPMFDPGNKASNASRIVAYGFRNPFRFTMRPGTNEAWVGNVGWGSWEEI
                     DRIQNPLASPAYNSGWPCLENETRLSGYRDLEVCQTIYHDTVDVADSPYLAYRHGDPI
                     NANDTCMIEDGTSISGAVFYTGDSYGSEYKNALFFADVSKNCIMYMPAGEDGLPDKND
                     VKVFVDDADDPRAVDLETDPASGDIFYVDIFGGKVHRISHPAGPANSVAPSITPAGTI
                     TSGDVLTASPGTWTGSQPIAFGYRWQRCSSSCSDIGGATSTQYTVQATDIGKKLRVNV
                     TATNPVGSSLAFAETGIVDNELPDAFIDLPTVSTNWKANDTITFSGHASDAEDGALPA
                     SALSWEIKIAHCPIACHEHPLATRTGTSGGTIAAPSHGAPSHLVFELTATDSAGESTT
                     VTRSIDPVVATLTFQTSPPGLTLTAGDQEAQAAPFTQTWVMNSQVQMSAPATQTVGGV
                     TYTFAGWSDAGAATHIVTADASATYTATYSAAPPPTTTTTTKPTTTTTTTTTTTTTTT
                     TTTTTTTTTATTTPPPPPATQSVPAGYWMVGGAGTAYAFGNVGHFGNAPTTSAVDLEP
                     TSSGKGYWIVDAGGHVFAFGDAHYFGTATTALAQGEVVTSLSRTKSGNGYWLFTNRGR
                     ALAFGDAPFLGDLRGLPLNGPVLDSVSTPSGNGYYMVASDGGVFSFGDAGFHGSMGGR
                     RLNAPVQSLVPDGDGAGYWLVASDGGIFAFDAPFHGSMGGQRLNRPVTGMVRFGDAYL
                     MVGEDGGIFNFSNQPFFGSLGSSPPPAPIVSVAASG"
     gene            complement(3285..3422)
                     /locus_tag="VFX21_08095"
     CDS             complement(3285..3422)
                     /locus_tag="VFX21_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095958.1"
                     /translation="MVRFGDGYLMVGEDGGIFNFSNQPFYGSLGSTPPAVPIVSVAAS
                     G"
BASE COUNT          520 a         1158 c         1256 g          583 t
ORIGIN      
        1 ggacgcgctc gacgagcagc cctacgactt cacggcgacg cagcaggcat tcgcggatgc
       61 cgaagtccgt ttccgcgaat cgagctagcc ggacgcggcg acggacacga tcggcgccgg
      121 cggcggcgac gaccccaacg agccgaagaa gggttggttg ctgaagttga agatgccgcc
      181 gtcctcgccg accatcaggt aggcatcgcc gaagcgcacc atccccgtga ccggacggtt
      241 gagtcgttgg ccgcccatcg acccgtggaa cggcgcgtcg aaggcgaaga tgccaccgtc
      301 ggacgccacg agccagtagc ccgcgccgtc gccgtcgggc acgagcgact gcacgggcgc
      361 gttcaaccgg cggccgccca tcgagccgtg gaacccggcg tcgccgaacg agaacacgcc
      421 gccgtcgctc gcaaccatgt agtagccgtt gcccgagggt gtcgagacgg agtcgagcac
      481 gggtccgttg agcggcaggc cgcgcaggtc gccgaggaac ggcgcgtcac cgaaggccag
      541 tgcgcggccc cggttcgtga acagccagta gccgttcccg ctcttcgtgc gcgacaagct
      601 cgtcacgacc tcgccttgtg cgagcgcggt cgtcgcggtg ccgaagtagt gcgcgtcgcc
      661 gaacgcgaag acgtgaccgc cggcgtcgac gatccagtac cccttcccgg acgatgtcgg
      721 ttcgaggtcg accgccgacg tggtgggcgc gttgccgaag tgcccgacgt tgccgaacgc
      781 gtacgcggtg cccgcgccac cgaccatcca gtagcccgcg ggcaccgact gcgtcgccgg
      841 cggaggcggt ggcgtggtcg tcgcggtcgt ggtcgtcgtg gtcgtggtcg tcgtggtcgt
      901 ggtcgtcgtg gtcgtggtcg tcgtggtcgt ggtcgtcggc ttcgttgtcg tggtcgtcgt
      961 tggcggcggg gccgcgctgt acgtcgcggt gtaggtcgcc gacgcgtcgg cggtgacgat
     1021 gtgcgtcgcc gcgccggcgt cggaccagcc cgcgaacgtg tacgtcacgc cgccgaccgt
     1081 ttgcgtcgcc ggcgcgctca tctgcacctg cgagttcatg acccacgtct gggtgaacgg
     1141 cgcggcctgc gcttcctggt cgccggcggt gagggtcaac cctggcgggc tggtctggaa
     1201 cgtcaatgtc gcgaccaccg gatcgatcga tcgggtgacg gtggtcgact cgcccgcgct
     1261 gtcggtcgcg gtgagctcga acacgaggtg cgacggcgcg ccgtgcgacg gcgccgcgat
     1321 cgtcccgccg ctcgtgcccg tgcgcgtcgc gaggggatgc tcgtgacacg cgatcgggca
     1381 gtgcgcgatc ttgatctccc aggacaatgc cgaagccggc aacgcgccgt cctccgcgtc
     1441 cgacgcatgg cccgagaacg tgatcgtgtc gttggccttc cagttcgtcg agacggtcgg
     1501 caggtcgatg aacgcatccg ggagttcgtt gtcgacgatc cccgtctccg cgaacgcgag
     1561 actcgaaccg accgggttcg tggcggtgac gttcacccgc agcttcttgc cgatgtccgt
     1621 ggcctgcacg gtgtactgcg tgctcgtcgc gccgccgatg tcgctgcacg acgacgagca
     1681 gcgctgccac cggtagccga aggcgatggg ttggctaccc gtccacgtcc ccggcgacgc
     1741 agtgaggacg tcaccggacg tgatcgtccc ggccggcgtg atcgacggtg cgacgctgtt
     1801 ggccgggccg gccggatggg agatgcggtg gactttgccg ccgaagatgt cgacgtagaa
     1861 gatgtcgccg gacgccggat cggtctccag gtcgaccgcg cgcgggtcgt ccgcgtcgtc
     1921 gacgaagacc ttgacgtcgt tcttgtcggg caagccgtcc tcgcccgccg gcatgtacat
     1981 gatgcagttc ttgctcacgt cggcgaagaa caacgcgttc ttgtactcgg agccgtacga
     2041 gtcgccggtg tagaacaccg cgccggagat cgaggtgccg tcttcgatca tgcacgtgtc
     2101 gttggcgttg atcgggtcac catgccggta cgcgaggtag ggactgtcgg cgacgtcgac
     2161 ggtgtcgtga tagatcgtct ggcacacctc gagatcgcgg taccccgaca gtcgcgtctc
     2221 gttctcgaga cagggccagc ccgagttgta cgccggcgac gcgagcgggt tctggatgcg
     2281 gtcgatctcc tcccacgatc cccacccgac gttgccgacc cacgcctcgt tggtcccggg
     2341 gcgcatggtg aagcggaagg ggttgcggaa cccgtaggca acgatgcgcg acgcgttcga
     2401 cgccttgttg ccggggtcga acatcgggtt gccgggggcg ccctcggcgt tggtggtgtc
     2461 ggggttgatc cgtaacagcg tgccgctcag caaccgcggc tcaccggggg gacggcgcac
     2521 gctttgcgcg cgcagcgcac cgccccgccc tgtcggtgac gtgttcgtca cgccgaccgc
     2581 gccgggcggg tcaccgcacg gattgacggg cgtcggcgtg cccggcaacg agccaccgaa
     2641 ctggccccag tcggcgctcg cataccccgc gccttcgccg ccggtgacgt acagcatgcc
     2701 gtccgcgccg aagtgcagat ctccgagcga gtgcgtcgtg aactgctggc accactcgtt
     2761 gtcgatcatc acctgctcgg cgcccgcggt gccgttcgcg ttgaccggga tacgggacaa
     2821 acggccgagg acgacgcaac cgtcggtgtc gccttccggc ggtgtcgggc agtcgtcacc
     2881 ccaatagggc gtgctctgtc ccggcggcgc gtccttcgcg tacgacacgt agatgtagtc
     2941 gtgccccgtg ccgttcccga gtcgcggatc gacggtgaga cccagcagac ctcggtccca
     3001 gaagttgaac acgttggtgg acagatcctt gaacagcgtc gcgctcgtgt cggcggtgtt
     3061 gtcgtagacc cagatctgcc cgctcttctc ggccaggaag accttgccgt tcggcgcgaa
     3121 cgcgaggttc gtcggcagcg tgcgcccgct ccacacgacg gtgtcggtga agccgtcggg
     3181 cagcgtcgac gcggcggcct gctcggtgac gagcggcagc gacatcgtgg cgagcagcag
     3241 agtcgccccg acgagcgcgc cgaacgcgca tcggcgattc cgcatcaccc ggacgccgcg
     3301 acggagacga tcgggaccgc gggcggtgtg gagccgagtg agccgtagaa gggttggttg
     3361 ctgaagttga agatgccgcc gtcctcgccg accatcaggt agccgtcccc gaagcggacc
     3421 atgccggtga tggccggttg agtcgttggc cgcccatcga cccgtggaac gcggcgtcga
     3481 aggcgaagat gccgccgtcg gatgcgacga gccagta
//
LOCUS       DASUGA010000407         2188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1407076, whole genome shotgun sequence.
ACCESSION   DASUGA010000407 DASUGA010000000
VERSION     DASUGA010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407076"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /gene="gltD"
                     /locus_tag="VFX21_08100"
     CDS             complement(<1..286)
                     /gene="gltD"
                     /locus_tag="VFX21_08100"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818196.1"
                     /note="glutamate synthase is composed of subunits alpha
                     and beta; beta subunit is a flavin adenine
                     dinucleotide-NADPH dependent oxidoreductase; provides
                     electrons to the alpha subunit, which binds L-glutamine
                     and 2-oxoglutarate and forms L-glutamate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HEX5095959.1"
                     /translation="MGETTGFLKWGRETPTRRAVDVRLRDWKEVYEPFPRDTLQLQAG
                     RCMDCGIPFCNNGCPLGNLIPDWNDLVYKGRWHDAVHRLQSTNNFPEFTGL"
     gene            complement(279..>2188)
                     /locus_tag="VFX21_08105"
     CDS             complement(279..>2188)
                     /locus_tag="VFX21_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEX5095960.1"
                     /translation="YGSISKEAHETLAIAMNRIGGKSNTGEGGEDPDRYIPDENGDLR
                     RSAIKQVASGRFGVTSEYLVNADDLQIKMAQGAKPGEGGQLPGGKVYPWIAATRHSTP
                     GVGLISPPPHHDIYSIEDIKQLIHDLKNSNPLARVHVKLVAQVGVGTVAAGVAKAHSD
                     VVLISGHDGGTGASPLTSIKHAGAPWELGLAETQQTLMRNGLRDRIVVQVDGQLKTGR
                     DVVIAALLGGEEFGFATAPLVVSGCVMMRVCHLDTCPVGIATQNPELRKRFTGKPEFV
                     VNFFEFLAQEVREYLAALGFRSIAEAIGHTEVLDMRPAVDHWKAHGLDLAPILLEAEN
                     PYDGQDRYATKAQDHGLDRALDQQLIALATPAIEDGKHVEIELPVQNTNRTVGTMLGS
                     LVTRTWGGDGLPDNTIRITLRGSAGQSFGAFLPSGITMRLEGDANDYCAKGLSGGRVI
                     LYPDRNATFVAEDNIIAGNVALYGATGGEAYIRGQVGERFCVRNSGATAVVEGVGDHG
                     CEYMTGGRVVVLGPTGRNFAAGMSGGIAYVYDPDEQFASLVNYEMVELVPLDDDDREW
                     LRSTIARHRELTGSDVADRLLGQWGSEVASFRKVMPEDFRRVLTVMREAEAAGLSEDE
                     TLAKVMESSHG"
BASE COUNT          327 a          738 c          741 g          382 t
ORIGIN      
        1 cgaggccggt gaattcgggg aagttgttcg tcgactgcag gcggtgcacg gcgtcgtgcc
       61 agcggccttt gtagacgagg tcgttccagt cggggatcag gttccccagc gggcatccgt
      121 tgttgcaaaa ggggatgccg cagtccatgc aacgaccggc ctggagctgc agcgtgtcgc
      181 gcgggaacgg ctcgtagacc tctttccagt ctcggagccg cacgtcgacg gcacggcgcg
      241 tgggcgtctc ccgaccccac ttgaggaagc cggtggtctc acccatgaga ggactccatc
      301 accttcgcca acgtctcgtc ttcggacagc cccgcggcct cggcctcgcg catcaccgtg
      361 agcacgcggc ggaagtcctc cggcatcacc ttgcggaacg acgcgacctc gctcccccac
      421 tgcccgagca accggtcggc aacgtcggag ccggtgagct cgcggtggcg tgcgatcgtc
      481 gaccgcagcc attcgcggtc gtcgtcgtcg aggggaacga gctcgaccat ctcgtagttg
      541 acgagcgatg cgaactgctc gtcggggtcg tacacgtacg cgatcccgcc cgacatcccg
      601 gccgcgaagt tgcgtccggt cggaccgagc acgacgacac gtccgccggt catgtactcg
      661 caaccgtggt cgcccacgcc ttcgacgacg gcggtggcgc cggagttgcg cacgcagaag
      721 cgttcgccga cctgaccgcg gatgtaggcc tcgcctccag tggcgccgta caacgcgacg
      781 ttcccggcga tgatgttgtc ctcggcgacg aacgtcgcgt tgcggtcggg atacaggatc
      841 acgcgcccgc ccgagagacc cttcgcgcag tagtcgttgg cgtcgccttc gagccgcatc
      901 gtgatgcccg acggcaggaa cgcgccgaag ctctgcccgg ccgagccgcg caacgtgatg
      961 cggatcgtgt tgtcgggaag gccgtcgccg ccccacgtgc gcgtgacgag cgagccgagc
     1021 atcgtgccga ccgtgcggtt cgtgttctgc acgggcagct cgatctcgac gtgcttgccg
     1081 tcctcgatcg cgggcgtggc cagcgcgatg agctgctggt cgagcgcgcg gtcgagaccg
     1141 tggtcctgcg ccttcgtggc gtagcggtcc tgaccgtcgt acgggttctc ggcctcgagc
     1201 aggatcggcg cgaggtcgag tccgtgggcc ttccagtggt cgaccgcggg tcgcatgtcg
     1261 agcacctcgg tgtgaccgat ggcctcggcg atcgaacgga acccgagcgc ggcgaggtac
     1321 tcgcgcactt cctgcgcgag gaactcgaag aagttcacga cgaactcggg cttgccggtg
     1381 aagcgcttgc ggagctcggg gttctgcgtc gcgatgccga cggggcacgt gtcgagatga
     1441 cagacgcgca tcatcacgca accactcacc acgagcggtg ctgtcgcgaa cccgaactcc
     1501 tcaccaccga gcagcgccgc gatcacgacg tcgcggccgg tcttcagttg tccatcgacc
     1561 tgcacgacga tgcggtcgcg aaggccgttg cgcatcagcg tctgctgcgt ctcggcgaga
     1621 ccgagctccc acggcgcgcc ggcgtgcttg atcgacgtga gcggtgacgc acccgtaccg
     1681 ccgtcgtgac ccgagatgag cacgacgtcg gagtgtgcct tcgccacacc cgcggccacg
     1741 gtgccgacac ccacctgcgc gacgagcttc acgtgcacgc gcgcgagcgg gttcgagttc
     1801 ttcaggtcgt ggatgagctg cttgatgtcc tcgatcgaat agatgtcgtg gtgtggcggg
     1861 gggctgatca ggccgacgcc cggtgtggag tgccgcgtgg ctgcgatcca cgggtacacc
     1921 ttcccacccg gcagctggcc gccctcgccc ggcttcgctc cttgcgccat cttgatctgg
     1981 aggtcgtcgg cgttgaccaa gtactccgac gtcacgccga accggcccga cgcgacctgc
     2041 ttgatcgccg atcgccggag gtcaccgttc tcgtcgggga tgtaccggtc ggggtcctcg
     2101 ccgccctcac ccgtgttcga ctttccgccg atccggttca tcgcgatggc gagcgtctcg
     2161 tgggcttcct tggagatgga gccgtacg
//
LOCUS       DASUGA010000408         5871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_281064, whole genome shotgun sequence.
ACCESSION   DASUGA010000408 DASUGA010000000
VERSION     DASUGA010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281064"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFX21_08110"
     CDS             <1..201
                     /locus_tag="VFX21_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX5095961.1"
                     /translation="AIKAPTPLHVPVTYEASLRAVDGRKLHVDATLSTAEGVLTATAD
                     AVFLAVDMEKVASTKRAAEAWA"
     gene            198..1151
                     /locus_tag="VFX21_08115"
     CDS             198..1151
                     /locus_tag="VFX21_08115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX5095962.1"
                     /translation="MSHRFRFGVLVSDVTSASRLHDDARKIEGLGYSTALVPDHLAVY
                     TPFAPLPVALSMANATSTLRVGTMVLANDLRHPALLARDVATIDVCSDGRFELGIGAG
                     WLRRDYDAAGIPYDAFGTRVERLAESLSVLKRLLEGDCVTHRGRHYTIDELDARPKTV
                     QRPRPPILVGGGSRAALELAAREADIVGVAPRMGLRAVDVGGLALRVQDAHGPATTDE
                     QLEWVRAAAGFRFDQLELQMFPMIVYVTPHREHLAEQLGAPVGLSEPDFLASSLALVG
                     TVDEIADELVARRERWGISYVVVPQVAADAFAPVVGRLAGT"
     gene            1232..2350
                     /locus_tag="VFX21_08120"
     CDS             1232..2350
                     /locus_tag="VFX21_08120"
                     /inference="COORDINATES: protein motif:HMM:NF018434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HEX5095963.1"
                     /translation="MLLAGFPGVAGHLTRLLAVAQARPRGFDAEFFVLDPGPFATHVT
                     DAGCTPVVTRTVPLDLWSVSDRDFAVRMLGAWFRRAVAEAMTVMKSIAPDCVVVDTHW
                     AAMAAATALDVPTLAVTNAVWTTHRRALGLADESDFELFAALFADAAAPALRAVGVGE
                     QALEALQHGTKTHIADLPALLGAPLGLDPDTTAGPLLWWPGASPRVEWPAGDGPTAYM
                     TAGSTGVLRDIDAAAMTLAERGWRVVVSGANDDGVVGNVVRTRFCDPRPLLETADVAV
                     LHGGSGSLLQALACGVPVVVVPANLDQLAHAELVERAGAGVMLGPAARAFTVDAVTDL
                     RDDEPARVRATELASMIRTEDATATVARTLSALAGVAV"
     gene            2347..3573
                     /locus_tag="VFX21_08125"
     CDS             2347..3573
                     /locus_tag="VFX21_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5095964.1"
                     /translation="MSGQSAVQWDRMVDGMMHAGDAPGEQTTYEDPYPRWAEMRAVAP
                     VHARGSGASPETLGHDVIVLGFDEAQLVLRDSERFATTVYGLLDPVMGRTILEMDGAE
                     HKTNRAFVMQAFTKAAIARWETDVIAPIVDAAVDKIVRGNGRADLVAELTFPVPFAVI
                     AGMLGLPSDELEALHDFAIDLIGAGIDERRAVHASERLRITLAEVIKDRRRTPQHDIV
                     STLAHARGPGRRLTDDEILSFLLLLLPAGGETSFRSTSTLLWTLLDDPARLAAVRADR
                     SLLPAAVEEGLRWEPPVVSMTRVARGDTELAGVPIAAGATIAACVGAANRDARYWENA
                     EQFDLHREPRPFLTFGSGPHLCLGLHLVRTETVSMITRVFDRLPGLRRDPDRPWPQVD
                     GVLLRSPSALPVVFDR"
     gene            3611..4138
                     /gene="def"
                     /locus_tag="VFX21_08130"
     CDS             3611..4138
                     /gene="def"
                     /locus_tag="VFX21_08130"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX5095965.1"
                     /translation="MATHAIRIFGDPVLKSPAAPVTDIDGALATLVDAMYDTMYDAPG
                     VGLAAPQVGVQKRFFVYDVADDTGPHVLLNPEIVEQTGEWLYEEGCLSLPGLAFEIVR
                     PKVVTVQGIDLDGNEVVVQGDELLGRVFLHEIDHLDGVLMLDRLDRGDRKRAMRELRE
                     QGMGVPTPGGRRHAL"
     gene            4151..5047
                     /gene="fmt"
                     /locus_tag="VFX21_08135"
     CDS             4151..5047
                     /gene="fmt"
                     /locus_tag="VFX21_08135"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX5095966.1"
                     /translation="MRLIFLGTPEGAVPPLRALVDAGHDVALVITQPDKRRSRGPGAD
                     ASPVKRAAEDLGLPVVTPEKSREIVEQVRDSDAAIGVVVAFGQLLPVSLLEAVPLGFV
                     NVHFSLLPRWRGAAPVERALLAGDAETGVCIMRVEAGLDTGPVYACERTPIGPDETAG
                     VLHARLVEMGTALLVRELPLVASATPVDQAGEATYADKLTVDEFRLDAARSADELARI
                     VRAGNPRPGAWFVAGGKRVKVLRAHVADASIPAGVVDPQGALGTARDTLVLDEVQPEG
                     KRPMTGEAWRRGVHGDVVLDTP"
     gene            5044..>5871
                     /locus_tag="VFX21_08140"
     CDS             5044..>5871
                     /locus_tag="VFX21_08140"
                     /inference="COORDINATES: protein motif:HMM:NF013216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX5095967.1"
                     /translation="MSDSRRVALDALIRIEEGAYAHLALPQMLTRSGLAPRDRGFATE
                     LVYGAVRMQRAVDYQLAKVCNRPLDSLEPVVRAALRLGAYQLLVGTAEHAAVGETVGA
                     VPERARGFVNGVLRGAVRARPWTWPPPTDTEGIGVRTSHPDWIVDLLVHEFGREDALA
                     TLALDDEAPPVTLRVNQRRATVGDVAAELTATGATVERGALVADALVVRDAGDIGALA
                     CVREGRASAQDQASQAVVALLGAQPGECVFDAAAAPGGKAAAIAEGIDDGGLVVAADL
                     "
BASE COUNT          786 a         2125 c         2104 g          856 t
ORIGIN      
        1 gcgatcaagg cgcccacacc cctgcacgta cccgtgacct acgaggcgtc cttgcgggcc
       61 gtcgacgggc gcaagctgca tgtcgacgcg acgctgtcga cagccgaagg cgtactgacc
      121 gcgacggccg atgccgtctt cctggcggtc gacatggaga aggtcgcgtc gaccaagcgc
      181 gcggcggaag cgtgggcgtg agccaccggt tccgcttcgg tgtgctcgtc tcggacgtca
      241 cgtccgcgag tcgcctgcac gacgacgcgc gcaagatcga ggggctcggc tactcgacgg
      301 cgttggtgcc cgaccacctc gcggtgtaca ccccgttcgc acccctcccg gtcgcgctct
      361 cgatggcgaa cgcgacgtcg acactgcgcg tcggcacgat ggtgctggcc aacgacctgc
      421 gccacccggc gctgctcgcg cgtgatgtcg ccacgatcga cgtctgctcc gacgggcgct
      481 tcgagctcgg catcggcgcg ggctggttgc ggcgcgacta cgacgccgcg ggcattccct
      541 acgacgcctt cggcacgcgc gtcgaacgac tggccgaatc gctgtcggtc ctgaagcgcc
      601 tcctcgaggg cgactgtgtg acgcaccgcg gccgtcatta cacgatcgac gagctcgacg
      661 cgcgcccgaa gaccgtgcag cggccgcgcc cgccgatcct ggtcggcggt ggttcgcgcg
      721 cggcgctcga gctcgccgca cgcgaggccg acatcgtcgg cgtcgcgccg cgtatggggt
      781 tgcgcgcggt cgacgtcggc ggcctcgcgc tgcgcgtcca agacgcgcac gggccggcca
      841 cgaccgacga acaactcgaa tgggtgcgcg cggcggccgg cttccgtttc gatcagctcg
      901 agctgcagat gttcccgatg atcgtctacg tcacgccgca ccgcgagcac ctcgcggaac
      961 agctcggcgc accggtcggg ctcagcgagc ccgacttcct cgcgtcgagc ctcgcgctcg
     1021 tgggaaccgt cgacgagatc gccgacgagt tggtcgcccg ccgcgaacgg tgggggatca
     1081 gctacgtcgt cgtcccgcag gtggcggccg acgcgttcgc gccggtcgtc ggaaggctcg
     1141 cgggtacatg atcttccctc ccgctccctc cggatcgcgg cctccggcat tcgcctccgg
     1201 tccgctgccc acccgaggcc gtgcaccgcg cgtgctgctg gccgggttcc cgggtgtcgc
     1261 ggggcacctg acgcgtctgc tcgcggtcgc gcaggcgcgc ccgcgcgggt tcgacgcgga
     1321 gttcttcgtc ctcgatcccg gaccgttcgc gacgcacgtg accgatgccg gctgcacacc
     1381 cgtcgtgacg cgcacggtgc cccttgacct gtggtcggtc tccgaccgcg acttcgcggt
     1441 gcggatgctg ggtgcgtggt tccggcgcgc cgtcgcggag gcgatgacgg tgatgaagtc
     1501 gatcgcgccc gactgcgtgg tcgtcgacac gcactgggcg gcgatggccg cggcgaccgc
     1561 gctcgacgtg ccgacgctcg ccgtcacgaa cgccgtgtgg acgacacacc gccgcgcgct
     1621 cggcctggcc gacgaatccg acttcgagct gttcgccgcg ctcttcgccg acgccgccgc
     1681 gcccgccctc cgcgcggtcg gcgtcggcga acaagcgctc gaggcgctcc agcacggcac
     1741 caagacgcac atcgccgacc tgcccgccct gctcggcgcg ccgctcgggc tcgatcccga
     1801 cacgacggcc ggcccgctgc tgtggtggcc cggcgcgtcg ccccgggtcg aatggcccgc
     1861 gggcgacggc ccgaccgcgt acatgaccgc ggggagcacc ggcgtcctgc gcgacatcga
     1921 cgccgccgcg atgacgctgg ccgaacgggg ctggcgcgtc gtcgtgagcg gcgcgaacga
     1981 cgacggcgtc gtcggcaacg tggtgcgcac ccggttctgc gatccgcgtc cgttgctcga
     2041 gaccgccgac gtcgcggtgt tgcacggcgg cagcggctca ctcctccaag cgctcgcctg
     2101 cggcgtcccc gtcgtcgtcg tgcccgcgaa cctcgaccag ctcgcgcacg cggagctcgt
     2161 cgagcgggcg ggcgcgggtg tcatgctcgg tcctgcggcg cgcgcgttca ccgtggatgc
     2221 cgtcaccgac cttcgtgacg acgagccggc gcgcgtgcga gccacggagc tcgcgtcgat
     2281 gatccgcacc gaggacgcga ccgcaacggt cgcacgcacc ctttcggctc tcgcaggagt
     2341 cgccgtatga gtggtcagag cgccgtgcaa tgggaccgga tggtcgacgg gatgatgcac
     2401 gccggtgacg caccgggcga acaaaccacg tacgaggacc cgtacccgcg ttgggccgag
     2461 atgcgcgcag tcgcacccgt gcacgcgcga gggagcggcg cgtcacccga gacactcggc
     2521 cacgacgtca tcgtgctcgg tttcgacgag gcacagctcg tgttgcgcga cagcgaacgg
     2581 ttcgcgacga ctgtgtacgg gctactcgat cccgtcatgg gccgcacgat cctcgagatg
     2641 gacggcgcgg agcacaagac gaaccgtgcg ttcgtcatgc aggcgttcac gaaggccgcg
     2701 atcgcccgct gggagaccga cgtcatcgca ccgatcgtcg acgcggccgt cgacaagatc
     2761 gtccgcggga atggccgggc cgacctcgtc gccgagctga cgttccccgt cccgttcgcg
     2821 gtcatcgcgg gcatgctcgg gctgccttcc gacgagctcg aggcgctgca cgacttcgcg
     2881 atcgacctca tcggtgcggg catcgacgag cggcgcgcgg tgcacgcgtc cgagcgcctg
     2941 cggatcacgc tcgcggaagt catcaaggat cgccgccgca caccgcaaca cgacatcgtg
     3001 agcacgctcg cgcacgcgcg cggcccgggc cggcggctca ccgacgacga gatcctctcg
     3061 ttcttgttgc tgctgctgcc cgcaggcggc gagacgtcgt tccggtcgac gtcgacgctg
     3121 ctgtggacgc tgctggacga tcccgcgcgg ctcgcggcgg tgcgcgccga ccggtcactg
     3181 ctcccggccg cggtcgagga aggcctgcgg tgggagccgc cggtcgtcag catgacgcgc
     3241 gtcgcccgcg gcgacaccga gctggcgggc gtgccgattg cggccggcgc gacgatcgca
     3301 gcgtgcgtcg gtgccgcgaa ccgtgacgcc cgctactggg agaatgccga gcagttcgac
     3361 ctccatcggg aaccacgacc gttcctcacg ttcggttccg gtccgcacct gtgtctcggc
     3421 ctgcacctgg tgcgcacgga gacggtgtcg atgatcaccc gcgtgttcga ccgcctgccc
     3481 ggattgcgcc gcgaccccga ccgcccgtgg ccccaggtcg acggcgtact cctccgttcg
     3541 ccgagcgcgc tccccgtcgt cttcgaccgg tagctgtggg cattcccggt gccattacga
     3601 tgcatgagtc atggccactc atgcgatccg catcttcggc gacccggtgc tcaagtcgcc
     3661 ggccgcgccg gtcaccgaca tcgacggcgc gctggccacg ctcgtcgacg cgatgtacga
     3721 cacgatgtac gacgcgcccg gcgtcgggct cgcggcgccg caggtcgggg tgcagaagcg
     3781 gttcttcgtg tacgacgtcg ccgacgacac cggaccacat gtgctgttga accccgagat
     3841 cgtcgagcag acgggcgagt ggctctacga agaaggttgc ttgtcgctgc cgggcctcgc
     3901 gttcgagatc gtgcgcccga aggtcgtcac cgtgcagggc atcgacctgg acggcaacga
     3961 agtcgtcgtg cagggcgacg aactgctggg ccgggtgttc ctgcacgaga tcgaccacct
     4021 cgacggcgtg ctcatgctcg accggctcga ccgcggcgat cgcaagcgcg cgatgcgcga
     4081 gctgcgcgag cagggcatgg gcgtccccac gcccggcggc cgccgccacg cgctctaagt
     4141 cggagtccgc gtgcggctga tcttcctcgg gacgcccgag ggcgccgtcc cgccgctgcg
     4201 cgcgctggtc gatgccggac acgacgtcgc cctggtcatc acgcaacccg acaagcgccg
     4261 ctcgcgtggt cccggcgccg acgcgtcgcc ggtcaagcgc gccgcggagg acctcgggct
     4321 gcccgtcgtc acgccggaga agtcgcggga gatcgtcgag caggtgcgcg actcggacgc
     4381 ggcaatcggc gtcgtcgtcg ccttcgggca actgctgccg gtctcgttgc tcgaggcggt
     4441 gccgctcggg ttcgtgaacg tgcacttctc gctgctgccg cgctggcggg gcgcggcacc
     4501 ggtcgagcgc gcgctgctcg ccggcgacgc ggagaccggt gtgtgcatca tgcgcgtcga
     4561 agccggtctc gacaccgggc cggtgtacgc atgcgagcgc acgccgatcg ggcccgacga
     4621 gacggcgggc gtgttgcacg cccggctcgt cgagatgggc accgcgctgc tcgtgcgcga
     4681 gctcccgctc gtcgcgtcgg cgacgccggt cgaccaagcg ggcgaagcca cgtacgcgga
     4741 caagctcacg gtcgacgagt tccgcctcga cgcggcgcgc agtgccgacg aactggcgcg
     4801 catcgtccgc gccggcaacc cgcgcccggg cgcctggttc gtggcaggtg gcaaacgcgt
     4861 caaggtgctg cgtgcccacg tggccgacgc ctcgataccc gccggtgtcg tcgacccgca
     4921 gggggcgctc ggcacggcgc gcgacacgct ggtgctcgac gaggtgcagc ccgagggcaa
     4981 gcggccgatg acgggcgagg catggcggcg cggggtgcac ggcgacgtcg tgctcgacac
     5041 accgtgagcg attcgcggcg cgtcgcgctc gacgcgctca tccgcatcga agaaggtgcg
     5101 tacgcgcatc tcgccctgcc gcagatgctc acccgcagcg gtctcgcgcc gcgtgaccgc
     5161 gggttcgcga ccgagctcgt gtacggcgcg gtccgcatgc aacgggccgt cgactaccag
     5221 ctcgcgaagg tctgcaaccg cccgctcgac tcgctcgaac cggtcgtgcg cgccgcattg
     5281 cggctgggcg cgtaccaact cctcgtcggc accgccgagc acgcggcggt gggtgagacg
     5341 gtcggcgcgg tccccgaacg ggcgcgcggg ttcgtcaacg gcgtgttgcg cggtgccgtg
     5401 cgggcccgtc cgtggacgtg gccgccgccc accgacactg aaggcatcgg tgtgcgcacg
     5461 tcgcatcccg actggatcgt cgatctgctc gtccacgagt tcggccggga ggacgccttg
     5521 gcgacgctgg ccctcgacga cgaagcgcca cccgtgacgc tgcgcgtcaa tcaacgccgc
     5581 gccaccgtcg gcgatgtcgc ggccgaactg accgcgacgg gtgccacggt cgagcgcggc
     5641 gcgctcgtcg cggatgccct cgtcgtgcgc gacgcgggtg acatcggcgc gctcgcgtgc
     5701 gtgcgcgagg gacgcgcgtc ggcgcaagac caagccagcc aggcggtcgt ggcactgctc
     5761 ggcgcgcagc cgggggagtg tgtgttcgac gcggccgccg caccgggcgg gaaggcggcc
     5821 gcgatcgcgg agggcatcga cgacggcggg ctcgtcgtcg cggccgacct g
//
LOCUS       DASUGA010000409         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1674628, whole genome shotgun sequence.
ACCESSION   DASUGA010000409 DASUGA010000000
VERSION     DASUGA010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674628"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="VFX21_08145"
     CDS             complement(<1..1127)
                     /locus_tag="VFX21_08145"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX5095968.1"
                     /translation="MTAVAHVGDVRVGLLGCGNVGGALARFVDEHADLIAARAGARVV
                     ITRVAVRDVTRPRDVKLPPSVFTDDAAAVVSDPDVDIVVEVIGGVEPARALVEAALRA
                     HKPVVTGNKELIARDGPELFRIAAEHGVDLLFEASVAGGIPLLRPLRESLAGDRIRRV
                     LGIVNGTTNYILTRMAEDATSFGDALAEAQRLGYAEADPTADVEGFDAAAKAAIIATI
                     AFGAPVGPGDVFREGIAQVTDDDMRSARSLGYVVKLLAVAEEAGGEISVRVHPAMVPV
                     HHPLAAVRDSFNAVFIEADAVGELMLYGRGAGGNPTASAVLGDLVDAARNLVGGARGA
                     TIGELVAKPIRPVGDVESPFYVLMEVADRPGVLHAISGEFSR"
     gene            complement(1160..>2009)
                     /gene="lysA"
                     /locus_tag="VFX21_08150"
     CDS             complement(1160..>2009)
                     /gene="lysA"
                     /locus_tag="VFX21_08150"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX5095969.1"
                     /translation="VLLRVTPGVEAHTHQFIETGTEASKFGFTVASGVARLAASRVAS
                     SDHLELAGLHCHIGSQIYVLDSYARAVQIVVALAGELEQAGIAIEELNFGGGLGARYL
                     ASDPALAIGEYARVLRDAVADARASTGLGAEPRLMVEPGRSIVAPAALTLYRVGTIKV
                     IPGIGTYVAVDGGMSDNPRPVLYGAGYEAFLPRAVAAARPLEVTVVGKHCEQGDILVA
                     GAQLPEDVAVGDLLVTPATGAYGYSMASNYNKVPRPAVVFVRDGEARVVVRRETLDDL
                     LRLDEG"
BASE COUNT          310 a          751 c          691 g          257 t
ORIGIN      
        1 cgcgagaact cgcccgagat cgcgtggagc acaccgggcc ggtcggccac ttccatgagc
       61 acgtagaacg gcgactcgac gtcgccgacc gggcggatcg gcttggccac cagctcgccg
      121 atcgtcgcgc cgcgcgcgcc accgacgagg ttgcgggcgg cgtcgacgag atccccgagc
      181 accgccgacg cggtcgggtt cccgccggcg ccgcggccgt acagcatgag ctcgcccaca
      241 gcgtcggctt cgatgaacac cgcgttgaac gagtcgcgta ctgccgcgag cgggtggtgc
      301 accggaacca tcgccgggtg cacccgtacc gagatctcgc cgccggcctc ctccgccacc
      361 gcgaggagct tcacgacgta cccgaggctg cgggccgatc gcatgtcgtc atcggtcacc
      421 tgcgcgatgc cctcacggaa gacgtcgccg gggccgaccg gcgcgccgaa cgcgatcgtg
      481 gcgatgatcg ccgccttcgc ggcggcgtcg aacccctcga cgtccgccgt ggggtcggct
      541 tcggcatagc cgagccgctg cgcctccgcg agcgcgtcgc cgaacgacgt ggcgtcctcg
      601 gccatgcgcg tgaggatgta gttggtggtg ccgttgacga tgccgaggac tcggcggatg
      661 cggtcgccgg caagcgactc gcgcagcgga cggagcaacg ggatgccgcc ggccaccgac
      721 gcctcgaaca gcaggtcgac accgtgctcg gccgcgatgc ggaagagctc cggaccatcg
      781 cgcgcgatga gctccttgtt cccggtgacc accggcttgt gcgcacggag cgccgcctcc
      841 accagtgcgc gcgcgggctc gacgccgccg atcacctcca ccacgatgtc gacgtcggga
      901 tccgacacga cggcggccgc gtcgtcggtg aacacgctcg gcggaagctt cacgtcgcgc
      961 ggacgggtga cgtcccggac ggcaacgcgc gtgatcacga cgcgcgcgcc cgcccgagcg
     1021 gcgatcaggt cggcgtgctc gtcgacgaag cgcgcgagcg caccgcccac gttcccgcac
     1081 ccgagcaagc cgactcgcac gtcacccacg tgtgccacgg ccgtcattgt gacgcaccat
     1141 ccgtcgcccg catgaatgat cagccctcgt ccaggcgcag caggtcgtcg agggtctccc
     1201 gccgcaccac cacacgcgcc tcgccgtccc gtacgaagac cacggccggc cggggcacct
     1261 tgttgtagtt cgacgccatc gagtacccgt acgcgccggt ggccggcgtc accaggaggt
     1321 ccccgaccgc gacgtcctcg ggcagctgcg caccggccac gaggatgtcg ccctgctcgc
     1381 agtgcttgcc gacgaccgtg acctccaacg gacgggcggc cgccacggca cgagggagga
     1441 acgcctcgta cccggcgccg tagagcaccg gacgcgggtt gtcgctcatg ccgccgtcga
     1501 ccgcgacgta ggtgccgatg cccgggatca ccttgatggt gccgacgcgg tagagcgtga
     1561 gcgcagccgg ggccacgatc gaccgacccg gttcgaccat cagccgcggc tcggcgccca
     1621 ggccggtgct cgcgcgcgcg tccgcaaccg cgtcgcggag cacacgcgcg tactcgccga
     1681 tcgcgagcgc agggtcactc gcgaggtagc gcgcgcccag gccacccccg aagttgagct
     1741 cctcgatcgc gatccccgcc tgctcgagct cccccgcgag cgccacgacg atctgcacgg
     1801 cacgcgcgta cgagtcgagg acatagatct gggagccgat gtggcagtgc aggcccgcga
     1861 gctcgaggtg atcactcgac gcgacgcgcg acgccgccaa gcgcgccacg ccggacgcca
     1921 cggtgaagcc gaacttcgat gcctcggtgc ccgtctcgat gaactggtgc gtgtgggcct
     1981 cgacgcccgg cgtgacgcgg aggagcaca
//
LOCUS       DASUGA010000410         3638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_162990, whole genome shotgun sequence.
ACCESSION   DASUGA010000410 DASUGA010000000
VERSION     DASUGA010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162990"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="VFX21_08155"
     CDS             complement(<1..1349)
                     /locus_tag="VFX21_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006539446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5095970.1"
                     /translation="MSADWSLPAALDAVTDAVPDREMLVWTSVRRTYADVQARTKRLA
                     AFFVARGLGVRHERAELQRWECGQDRVAVVLSNCPEYVETMIGAYRARIVPYNVNHHY
                     LPAEVAALLDQIGTQAIVYHLRLGPLLADVGLEGRVLVHVDDGSGVAPLPGSVAYEDA
                     IANARAVDALPEPSADDLYLLCTGGTTGRPKGVVWRQGDAYVAAMGGAEGATAASIAA
                     CADRPVPVWFAAPPLMHGAAQWTVFACLHNGGTVVLHDDAGPFSPSTILETAARERAT
                     MLTIVGDAYARPIIDELKAGTYDLSGLAQLSTGGAMTSPVLKAAFLDLLPHLTIVDGY
                     GASETGGMAFGASRKNAQTQQFSGSSGAAVLSEDRTRFLEAGDDEMGWTARIGRVPLG
                     YLDDREATERTFPIVDGRRLAVPGDRAKVDADGTIILFGRDSMVVNSGGEKIFVEEV"
     gene            complement(1349..2548)
                     /locus_tag="VFX21_08160"
     CDS             complement(1349..2548)
                     /locus_tag="VFX21_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5095971.1"
                     /translation="MAVSESLPLSYDPFDVEIDRNVHTVWRRMREEQPVYWNEKYEFY
                     ALSRFEDVWSAYHDTATFSSTHGVMLESLDEPIGMPMVIFMDPPEHDWMRKLVSRAFT
                     PRRIADLEAHVAELTDAYLDPFVGSAGFDYVSQFGALLPPMVIGHMIGVDEADRDMVR
                     RWFDESLHREPGSPMPSDEATAAISSILEYANQMIAERRKHPRDDMVTVLLEAEVQDG
                     ATTRRLDDLEVALFIMLLVGAGVETVARLLSFAAVTLAENRDQRALLVDDPSLIPNAV
                     EELLRYEAPSPVNGRWTLAPSEFHGVEIPAGSKVLLLNGSANRDPREFDDPDRFDVRR
                     RIKRHISFGYGAHFCLGAALARMEARIALTGTLARFPHWDVDRSEIVPVITTTVRGYS
                     SVPIHFR"
     gene            2606..>3638
                     /locus_tag="VFX21_08165"
     CDS             2606..>3638
                     /locus_tag="VFX21_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020460280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5095972.1"
                     /translation="MANQLRPIDADNHYYEPLDAFTRHLDKKFKQRGVRAVHDGSHVE
                     MIIGGKLNRFIPNPTFDPVIVPGCLDQQFRGEIPEGVDPRTLVRVEPISDTYRNRDAR
                     LAKMDEQQLDAVLLFPTLGCGVEQALMHDIPATMASLHAFNRWLEDDWGYSYRDRIVA
                     APMISLADPVAAVEEVDRVLGLGARVLHMRPAPVPTGGAKGRSLGHKAHDPVWARVAE
                     ANIPVAFHLGDSGYNALLGAPWGGAEDFAPFREPDLLGSVIIGDRAIHDTMASLIVHG
                     VFTRHPTLRVASIENGSDWIYPLVKGLKKQANRTPWLFAQDPLDTIKHHVWVTPYYEE
                     DLRKLADTIG"
BASE COUNT          594 a         1252 c         1226 g          566 t
ORIGIN      
        1 tcgacttcct cgacgaagat cttctcgccg ccgctgttga cgaccatcga gtctcgcccg
       61 aacaagatga tggtgccgtc ggcgtcgacc ttggcccggt cgcccgggac cgcgagccgg
      121 cgcccgtcga cgatcgggaa cgtgcgctcg gtcgcctcgc ggtcgtcgag gtatccgagg
      181 ggcacgcggc cgatgcgcgc cgtccatccc atctcgtcgt caccggcctc gaggaatcgc
      241 gtgcggtcct ccgacagcac cgcggcgccg ctcgacccgg agaactgctg cgtctgcgcg
      301 ttcttgcgcg acgcgccgaa ggccatgccg cccgtctcgg acgcgccgta gccgtcgacg
      361 atcgtgaggt gcggcagaag atcgaggaac gccgctttca gcaccgggct cgtcatcgcg
      421 ccgccggtcg acagctgcgc caacccggac aggtcgtagg tgcccgcctt cagctcgtcg
      481 atgatcggcc gcgcgtacgc atcgccgacg atcgtgagca tggtggcgcg ctcgcgcgcc
      541 gcggtctcca ggatcgtcga aggcgaaaac ggaccggcgt cgtcgtggag cacgacggtg
      601 ccgccgttgt gcaagcacgc gaacaccgtc cattgggccg cgccgtgcat gagcggcggt
      661 gccgcgaacc acaccggtac cggccggtcc gcgcacgccg cgatcgacgc ggccgtcgcg
      721 ccctcggcgc cacccatcgc ggcgacgtac gcgtcacctt gccgccacac cacgcctttc
      781 ggccggcccg tcgtgccgcc ggtgcacagc agatagaggt cgtcggctga cggttcgggt
      841 aacgcgtcga cagcgcgcgc gttcgcgatc gcgtcctcgt aggcgacgct tccggggagc
      901 ggcgcgacac ccgagccgtc gtcgacgtgc accaacaccc gaccttcgag cccgacgtcg
      961 gcgagcagcg ggccgaggcg caggtggtac acgatcgcct gcgtgccgat ctggtcgagc
     1021 agcgccgcga cctccgccgg gaggtagtgg tggttgacgt tgtacggcac gatgcgggcc
     1081 cggtacgcgc cgatcatcgt ctcgacgtac tcgggacagt tcgacagcac gacggcaaca
     1141 cggtcctgcc cgcattccca gcgctgcaac tcggcgcgtt cgtgccgcac accgagcccc
     1201 cgcgcgacga agaacgcagc gagacgcttc gtgcgcgcct gcacgtcggc atacgtgcgc
     1261 cggaccgacg tccacacgag catctcgcga tcgggcaccg cgtcggtgac cgcgtcgagc
     1321 gcggccggca acgaccagtc cgcgctcatc agcggaagtg aatcgggacc gacgagtagc
     1381 cgcgcacggt tgtggtgatc accggcacga tctcggaccg gtcgacgtcc cagtgcggga
     1441 accgcgcgag cgtccccgtc agcgcgatgc gcgcttccat gcgggcgagc gcggcaccga
     1501 gacagaagtg tgcgccgtac ccgaacgaga tgtgccgctt gatccggcgc cgcacgtcga
     1561 agcggtcggg atcgtcgaac tcgcgcgggt cgcgattcgc gctgccgttc agcaacagca
     1621 ccttcgaccc ggccgggatc tccacgccgt ggaactcgga cggcgccagc gtccaccgtc
     1681 cgttcacggg cgagggcgcc tcgtagcgca gcagctcttc gaccgcgttg gggatgagcg
     1741 acggatcatc gacgagcagt gcgcgttggt cgcggttctc ggccagtgtg actgcggcga
     1801 agctgagcag ccgcgccacc gtttcgacgc ccgcgccgac gagcagcatg atgaacagcg
     1861 cgacctcgag gtcgtcgagg cggcgcgtcg ttgcaccgtc ctgcacttcc gcttcgagca
     1921 gcaccgtgac catgtcgtcg cgcgggtgct tccggcgctc ggcgatcatc tggttggcgt
     1981 actcgaggat cgaggagatg gccgccgtcg cttcgtcgct cggcatcggg ctgcccggtt
     2041 cgcggtgcag cgactcgtcg aaccagcgcc ggaccatgtc gcggtcggct tcgtcgacac
     2101 cgatcatgtg gccgatcacc atcggcggca acagtgcgcc gaattgcgag acgtagtcga
     2161 agcccgccga cccgacgaag gggtcgaggt acgcgtcggt cagttcggcg acgtgcgctt
     2221 cgaggtccgc gatacgacgg ggcgtgaacg cgcggctgac gagcttgcgc atccagtcgt
     2281 gctcgggcgg gtccatgaag atgaccatgg gcatgccgat cggctcgtcg agcgattcga
     2341 gcatcacgcc gtgcgtcgag ctgaacgtcg cggtgtcgtg gtacgcgctc cacacgtctt
     2401 cgaagcgact cagcgcgtag aactcgtact tttcgttcca gtagacgggc tgttcttcgc
     2461 gcatccgtcg ccatacggtg tggacgttgc ggtcgatctc gacgtcgaac gggtcgtacg
     2521 acagtggcaa cgactcactg acggccatgc gttgacccta gcaaaagatg tagatgtata
     2581 ttcagggccg tcgaggagga tcgccatggc gaaccaactg cgccccatcg atgccgacaa
     2641 ccactactac gagccgctcg acgcgttcac gcgccacctc gacaagaagt tcaagcagcg
     2701 cggcgtccgc gccgtgcacg acggttcgca cgtcgagatg atcatcggcg gcaagctgaa
     2761 ccgcttcatc ccgaacccga cgttcgaccc ggtgatcgtg ccgggatgtc tcgaccagca
     2821 gttccgcggc gaaatccccg aaggtgtcga cccgcgcacg ctcgtgcgcg tcgagcccat
     2881 ctccgacacc taccgcaacc gcgacgcgcg cctcgcgaag atggacgagc agcagctcga
     2941 cgcggtgctg ttgttcccca cgctcggctg tggcgtcgag caggcgttga tgcacgacat
     3001 cccggcgacg atggcgagcc tgcacgcgtt caaccgctgg ctcgaagacg attggggcta
     3061 ctcgtaccga gaccggatcg tcgccgcgcc gatgatctcg ctggcggacc cggtggccgc
     3121 ggtcgaggag gtcgatcgcg tcctcggact gggcgcgcgc gtcctgcaca tgcgccccgc
     3181 gccggtccct acgggtggtg cgaagggtcg ctcactcggc cacaaggcgc acgacccggt
     3241 gtgggcgcgc gtggccgagg cgaacatccc ggtcgcattc catctcggcg acagcggata
     3301 caacgcgttg ctcggcgcgc cgtggggcgg cgccgaggac ttcgcgccgt tccgtgagcc
     3361 cgacctgctc gggtccgtga tcatcggcga ccgcgcgatc cacgacacga tggcgagtct
     3421 catcgtgcac ggcgtgttca cgcggcaccc gacactgcgc gtcgcgagca tcgagaacgg
     3481 gtccgactgg atctacccgc tggtgaaggg actgaagaaa caggcgaacc gaacgccgtg
     3541 gctcttcgcg caggatccgc tcgacacgat caagcaccac gtatgggtca cgccctacta
     3601 cgaagaggac ctgcggaagc tggccgacac gatcggtg
//
LOCUS       DASUGA010000411         3141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_250402, whole genome shotgun sequence.
ACCESSION   DASUGA010000411 DASUGA010000000
VERSION     DASUGA010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250402"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VFX21_08170"
     CDS             <1..455
                     /locus_tag="VFX21_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095973.1"
                     /translation="DGQTWDGVVEPDADIHEVVAADGHVVVAAAVGFGWSDDGTTWRW
                     TTDGLHASYARAAAIDGDTFFVSASNGPFTRRGAVYRARIGGRFERCTAGLPEWFPGN
                     VDSGALAARDGRAAIGTYDGEVYVSGDAGATWELAADDLGSVRAVRFA"
     gene            complement(468..1637)
                     /gene="xylA"
                     /locus_tag="VFX21_08175"
     CDS             complement(468..1637)
                     /gene="xylA"
                     /locus_tag="VFX21_08175"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018519755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEX5095974.1"
                     /translation="MSGAFEPTPADRFTFGLWTVGNRGRDPFGHETRPPLDPVDSVRR
                     LADLGAYGVNFHDDDLVPPGSSAAEREQILKRFRRALDDTGMKVPMATTNLFSNPVFK
                     DGAFTANDPMVRRYAVAKTLDAIDLGVELGAGIYVMWGGREGAECDAAKDIPLALDRY
                     REAVDLCCAYVLDRGYEVRFALEPKPNEPRGNILLPTVGHALGFIEQLAWPEMVGLNP
                     EFAHETMSGLSFHHGVAQTLWAGKLFHIDLNGQVPGKYDQDFRFASEGVKDAFYLVRL
                     LERSGWTGMRHFDAHAYRTEDEAGVWEFARGCMRSYLILKDKARRFDEDTEIQAALQD
                     AMVDKLAERTSPDGASADAIAALRRVSYDVETLAAQGYGHERLDQLVTELLLGVR"
     gene            1872..2549
                     /locus_tag="VFX21_08180"
     CDS             1872..2549
                     /locus_tag="VFX21_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095975.1"
                     /translation="MPAASSSSDRVRYAWQRRHESDYIFDFWTALGWTILTCGIYGLY
                     IVYQLVRRSRDHLLRRIELLDAATAFAWEQAEARGLSEELRPGFERIAQNMQTMRRQT
                     TEFREPVVWMILSLFASTIVYIVLFCLLDGDLVTHDHTEGAIEADLSEIYARLGAPVP
                     PPNPGRLKGPHNYVGRIIATLATCGIYGLWWEYDIMVEGNRHFEEAWRWEDGLANSVQ
                     SLLAAAA"
     gene            2564..3067
                     /locus_tag="VFX21_08185"
     CDS             2564..3067
                     /locus_tag="VFX21_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase / dehydrase and lipid
                     transport family protein"
                     /protein_id="HEX5095976.1"
                     /translation="MQRHEFSVDLDAAPADVWEVFWYRAPDRPQPRDVDTRIDILHPG
                     DEDGNGLVRHCTFPVPKWLLSKGVGQSWEWLTEVKPYESWRYDAIGKPLWSRARGFTR
                     LEDLGDGRTRVHFSEEYEAFNPIMRALFEKRVHAFLSRDNDTILAALQGGIRWHQKRR
                     RREASAG"
BASE COUNT          482 a         1091 c         1080 g          488 t
ORIGIN      
        1 acgacgggca gacgtgggac ggcgtcgtcg aacccgacgc cgacatccac gaggtcgtcg
       61 ccgccgacgg gcacgtcgtc gtcgcggccg cggtcggatt cggctggagc gacgacggca
      121 cgacgtggcg gtggaccacc gacgggcttc acgccagtta tgcgcgtgcc gccgcgatcg
      181 acggcgacac gttcttcgtg agcgcgtcga acggcccgtt cacgcgccgc ggtgcggtgt
      241 atcgcgcgcg catcggcgga cgcttcgaac gctgcacggc cggcctgccc gagtggttcc
      301 ccggcaatgt cgacagcggt gcgctcgccg cgcgcgacgg ccgcgccgcg atcggaacgt
      361 acgacggcga ggtctacgtg tcgggcgatg ccggcgcgac gtgggagctg gcggccgacg
      421 acctcggctc cgtccgcgcg gtgcgcttcg cgtaggggaa cgctcgccta gcggacgccc
      481 aacaacaact cggtcaccaa ctggtcgagg cgctcgtgtc cgtatccctg cgcggcgagt
      541 gtctcgacgt cgtatgagac ccggcgcaac gccgcgatcg cgtccgccga cgcgccgtcg
      601 ggtgacgtcc gctcggccag cttgtcgacc atcgcgtcct ggagcgcggc ttggatctcg
      661 gtgtcttcgt cgaagcgacg ggccttgtcc ttcaggatga gatagctgcg catgcaaccg
      721 cgcgcgaatt cccacacacc ggcttcgtcc tcggtgcggt acgcgtgcgc gtcgaagtgc
      781 cgcatgccgg tccagcccga gcgttcgagg agacgcacga ggtagaacgc gtccttcacg
      841 ccctccgacg cgaaccggaa gtcctggtcg tacttgcccg gcacctgtcc gttgaggtcg
      901 atgtggaaca gctttccggc ccacaacgtc tgcgcgacac cgtggtggaa cgagagcccc
      961 gacatcgtct cgtgcgcgaa ctcggggttg agcccgacca tctccggcca cgcgagctgc
     1021 tcgatgaaac cgagcgcgtg cccgactgtg gggaggagaa tgttcccgcg cggctcgttc
     1081 ggcttgggtt cgagcgcgaa ccgcacctcg tagccgcggt cgagcacgta tgcgcagcag
     1141 aggtcgaccg cttcgcgata ccgatccagc gcgagcggga tgtctttggc cgcgtcgcac
     1201 tcggcgccct cacgcccgcc ccacatgacg tatatgccgg cgccgagctc gacgccgagg
     1261 tcgatcgcgt cgagcgtctt ggccacggcg taacgccgca ccatcggatc gttggcggtg
     1321 aacgcgccgt ctttgaacac ggggttcgag aacaggttcg tcgtcgccat cggcaccttc
     1381 atgccggtgt cgtcgagcgc gcgccggaat cgcttcagga tctgctcgcg ctcggccgcg
     1441 ctcgagcccg gcggcaccaa gtcgtcgtcg tggaagttca cgccgtacgc gccgaggtcg
     1501 gcgaggcggc ggacggagtc gaccggatcg aggggcggtc gcgtctcgtg cccgaacgga
     1561 tcgcgcccgc gattgccgac ggtccacaac ccgaaggtga accggtcggc cggtgtcggt
     1621 tcgaacgcgc cgctcatgcc acatctccct gcgcggggtc cctgcgcgtg gtgcgggcac
     1681 ccagcgtatg ctcgcatccc gtgatgccgc cgccggactc ccccaacgga ccgaacggac
     1741 cgaacgggcc cagcgagccc aacgatgcgc cgccgcccac agggccggcg gacgcgccgc
     1801 cgagcgcgtg gacgggaccg gccacgccac cgccgatggg cgggccgtcg ggcccgccgc
     1861 aacaaccctg gatgcctgcg gcgtcgagct cgtcggatcg cgtccggtac gcgtggcagc
     1921 gccggcacga gagcgactac atcttcgact tctggaccgc gctcgggtgg acgatcctca
     1981 cgtgcggcat ctacggcctc tacatcgtct accaactcgt tcgccgcagc cgcgaccacc
     2041 tgctgcggcg catcgagctg ctcgacgccg cgaccgcgtt cgcgtgggag caagccgaag
     2101 cgcgcggcct gtccgaggag ctgcgtccgg ggttcgagcg catcgcgcag aacatgcaga
     2161 cgatgcggcg gcagacgacc gagttccggg agcccgtcgt ctggatgatc ctctcgctgt
     2221 ttgcgagcac catcgtctac atcgtgttgt tctgcctgct cgacggcgac ctcgtgacgc
     2281 acgaccacac cgaaggcgcg atcgaagccg acttgtccga gatctacgcg cggctcggcg
     2341 caccggtgcc gccgccgaat ccgggccggc tgaagggccc gcacaactac gtcggccgca
     2401 tcatcgccac gctcgcgacg tgcggcatct acggcctctg gtgggagtac gacatcatgg
     2461 tcgaaggcaa ccggcacttc gaagaagcgt ggcgctggga agacggtctc gcgaactccg
     2521 tgcagtcgct gctggcggcc gcggcctagc gtccggtgca gtgatgcagc ggcacgagtt
     2581 ctcggtcgac ctcgacgccg ctcccgcgga cgtgtgggaa gtgttctggt accgcgcgcc
     2641 ggatcggccg caaccgagag acgtcgacac gcgcatcgac atcctgcacc cgggagacga
     2701 ggacgggaac ggactcgtgc gccattgcac gttccccgtt cccaagtggc tgttgtcgaa
     2761 aggcgtcggc cagtcgtggg agtggctgac cgaagtcaag ccgtacgagt cgtggcgtta
     2821 cgacgcgatc ggcaaaccgt tgtggtcgcg cgcccgcggc ttcacgcgcc tcgaagatct
     2881 cggtgacggc cgcacgcgcg tgcacttctc ggaggagtac gaggcgttca atccgatcat
     2941 gcgcgcgctc ttcgagaagc gcgtgcacgc gttcttgtcg cgcgacaacg acacgatcct
     3001 cgccgcgctg cagggcggca tccgctggca ccagaagcga cgccggcgcg aagcatccgc
     3061 cggttgagca cggtcgtcct cttcgtctac ggcacgctcg cgccgggcca ggaggcgtgg
     3121 tcgaccctgg cgccgcatgc c
//
LOCUS       DASUGA010000412         3471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1974552, whole genome shotgun sequence.
ACCESSION   DASUGA010000412 DASUGA010000000
VERSION     DASUGA010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1974552"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /locus_tag="VFX21_08190"
     CDS             <1..1363
                     /locus_tag="VFX21_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013426432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX5095977.1"
                     /translation="AGDVVGLLSYNSIDFLTTIFAANHLGAIAMPVNWRLAAPEVQFI
                     LEHSQARAFVCDGELLDLGNEATKDASSDLVRVSIGPRADVWTDFEELAASEARVARI
                     AVAGDDIHRLMYTSGTTGRPKGVMITHANLAWKNFAHVTEFGFTSADIGLACGPLYHV
                     GALDLVTTTMIAVGATTIVHRVFDAQRVVDDIERLRITTVWTAPAMVRAILDLPGLEQ
                     RDLSSVRVIIAGGEKMPIPFIERLRKTFPSAWFADAYGLTETVSGDTFLDREHTITKL
                     GSVGRVCQYLELDIWDEDGNSLPPDTPGEIVLRGPKVFKGYWRDPAATTKAFAGGWFH
                     TGDIGVQDAEGYVYIVDRLKDMIVSGGENIASSEVERVLYEHDSVVEVAVVGRPDERW
                     GEVPVAYVVRQDGATTTADELIEHCRTQLAKFKVPKDVTFIDALPRNPSGKVLKRELR
                     SQS"
     gene            1360..2226
                     /locus_tag="VFX21_08195"
     CDS             1360..2226
                     /locus_tag="VFX21_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5095978.1"
                     /translation="MKQELCDFSDEVYTRIRARLDGLTDDEYFWEPAPGCWTIRRRAD
                     GTWMCDFPLPRPDIEPFTTIAWRLWHLIDMYGENRAPQWLAVAPQGEAIGRDAPDTTP
                     PSTAAAAITLLERAHDRWDAHLALATEDSLREPIGPVGGDYAEKSRIAYVHHMLDEFV
                     HHGAEIAVLRDLWRWQHPIADPLHELVMRGDMSVLDDAAPSADLVSLAAQYGRWDLML
                     GLLGRGAPLPTTGRLPLQLAAGAGELEVVKRLIEHGADPSATDDEYHATPLVWAEFLN
                     QPAVADYLKGAT"
     gene            complement(2210..3088)
                     /locus_tag="VFX21_08200"
     CDS             complement(2210..3088)
                     /locus_tag="VFX21_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013426429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5095979.1"
                     /translation="MSEHRFDGRVAVVTGAGRGIGRAYAHLLAALGAEVVVNDLGSSM
                     EGDGVDAGPAFEVVAEISAAGGTALADTNDVSTPAGAHALVDWAVAEYGRIDALINNA
                     GIMRWAGMPDVDAAQLDQHLAVHVGGSFNTTRAAWPHMVEQGYGRIVMTTSAGVFGLP
                     KNTAYATAKGGVIGLARSLATAGAQHGIKVNVIAPAAYTRMARGPDAGGMQPELVAPM
                     AAFLAHEDCPVNGEIYLAGAGRFSRIFLATTEGYVNNAATIEDVARHWTEINDETGYA
                     VPADLIAWAEQFTSHL"
     gene            3121..>3471
                     /locus_tag="VFX21_08205"
     CDS             3121..>3471
                     /locus_tag="VFX21_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013426435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5095980.1"
                     /translation="MPATESQRSEKWKARRDQIVDTSAKVFARGGYHATGIAELCTVN
                     GLGKGAFYHYIGSKEELLAAIHDRVMDEVMAGADRVAASGAPPAAQLAMLGDELLDVI
                     RRYPDHVWVFLHEFP"
BASE COUNT          553 a         1208 c         1161 g          549 t
ORIGIN      
        1 cgccggcgac gtcgtcgggc tcctctccta caacagcatc gacttcctga cgacgatctt
       61 cgccgccaat cacctgggtg cgattgcgat gccggtcaac tggcggctcg cggctcccga
      121 agtgcaattc atcctcgagc actcgcaggc gcgcgcgttc gtgtgcgacg gcgaactgct
      181 cgacctcggg aacgaagcca cgaaggacgc atcgagtgat ctcgtccgcg tctcgatcgg
      241 tccgcgggcc gacgtctgga ccgacttcga ggagctggct gcgagtgagg cgcgcgtcgc
      301 ccggatcgcc gtcgcgggtg acgacatcca ccggctgatg tacacctccg gcacgaccgg
      361 ccggcccaag ggcgtgatga tcacgcacgc gaaccttgcg tggaagaact tcgcgcacgt
      421 caccgagttc gggttcacga gcgccgacat cgggctcgcg tgtgggccgc tgtaccacgt
      481 gggcgcgctc gacctggtca cgacgacgat gatcgcggtg ggcgcgacga cgatcgtcca
      541 ccgcgtgttc gacgcgcagc gggtcgtcga cgacatcgaa cggctgcgga tcaccaccgt
      601 atggaccgcg ccggcgatgg tgcgcgcgat cctcgacctc cccggtctcg agcagcgcga
      661 tctttcgtcg gtgcgcgtga tcatcgcggg cggcgagaag atgccgatcc cgttcatcga
      721 acgattgcgc aagacgttcc catcggcgtg gttcgcggac gcgtacggct tgaccgagac
      781 cgtgtccggc gacacgtttc tcgaccgcga gcacacgatc accaagctcg gcagcgtcgg
      841 tcgcgtgtgc cagtacctcg agctcgacat ctgggacgag gacggcaact cgttgcctcc
      901 ggacacgccg ggcgagatcg tgctgcgcgg ccccaaggtg ttcaagggct attggcgcga
      961 ccccgccgcg accaccaagg cgttcgcggg cggctggttc cacaccggcg acatcggtgt
     1021 gcaggacgcc gagggctacg tgtacatcgt cgaccgcctc aaggacatga tcgtgtccgg
     1081 cggcgagaac atcgccagct ccgaagtcga gcgcgtgctg tacgagcacg attccgtcgt
     1141 cgaggtcgcg gtcgtcggcc gccccgacga gcgttggggc gaggtgccgg tcgcctacgt
     1201 cgtgcggcag gacggtgcga cgacgaccgc cgacgagctg atcgagcact gccgtacaca
     1261 gctggcgaag ttcaaggtgc cgaaggacgt gacgttcatc gacgccctcc cccgtaatcc
     1321 ctcgggcaag gtgctgaagc gagaattacg ctcacagtcg tgaagcaaga gctgtgcgac
     1381 ttctcggacg aggtgtacac gcgcatccgc gctcgcctcg acggcctcac cgacgacgag
     1441 tacttctggg agccggcacc gggctgttgg acgatccgcc ggcgcgccga cggcacgtgg
     1501 atgtgcgact tcccgttgcc gcgcccggat atcgagccgt tcacgacgat cgcgtggcgg
     1561 ttgtggcacc tcatcgacat gtacggcgag aatcgcgcac cgcagtggct cgctgtcgcg
     1621 ccgcagggcg aagcgatcgg gcgtgacgca cccgacacga cgcccccgtc gaccgcggct
     1681 gcggccatca cgttgttgga gcgcgcgcac gaccgctggg acgcgcacct tgcactcgcg
     1741 acggaggaca gcttgcgcga accgatcgga ccggtcggcg gcgactacgc cgagaagtcg
     1801 cgcatcgcgt acgtgcacca catgctcgac gagttcgtgc accacggcgc cgagatcgcg
     1861 gtgctgcgcg acctgtggcg ctggcaacat ccgatcgccg atcccttgca cgaattggtc
     1921 atgcgcggcg acatgtcggt gctcgacgac gccgcgccga gcgcggacct cgtctcactt
     1981 gccgcgcaat acggccgctg ggacctgatg ctcgggctgc tcggacgcgg cgcgccgctc
     2041 cccacaaccg gacggctgcc gttacagctg gcggccggcg cgggcgagct cgaggtggtg
     2101 aagcgcctga tcgaacacgg cgccgatccc agcgccacgg acgacgagta ccacgcaacc
     2161 ccgcttgtgt gggccgagtt cctcaaccaa cccgccgtcg ccgactacct caaaggtgcg
     2221 acgtgaactg ctcggcccag gcgatgaggt cggcgggcac tgcataaccc gtctcgtcgt
     2281 tgatctcggt ccagtgccgg gcgacgtctt cgatcgtcgc cgcgttgttg acgtagccct
     2341 ccgtcgtggc gaggaagatg cgcgagaacc ggcccgcgcc cgcgagatag atctcgccgt
     2401 tcacggggca gtcctcgtgc gcgaggaacg cggccatggg cgcgacgagc tccggctgca
     2461 tgccgccggc gtcgggtccc ctcgccatgc gcgtgtatgc ggcgggcgcg atgacgttca
     2521 ccttgatgcc gtgttgcgcg cccgcggtcg cgaggctgcg cgccagtccg atcacgccgc
     2581 ccttcgcagt cgcgtatgcg gtgttcttcg gcaggccgaa cacgccggcc gacgtcgtca
     2641 tgacgatccg gccgtagccc tgctcgacca tgtgcggcca cgccgcacgc gtcgtgttga
     2701 aggaaccacc cacgtgtacc gcgaggtgtt ggtcgagttg cgcggcatcg acgtccggca
     2761 taccggccca gcgcatgatg ccggcgttgt tgatcaacgc gtcgatgcgg ccgtactcgg
     2821 cgaccgccca gtcgacgagc gcgtgcgcgc ccgccggtgt cgacacgtcg ttcgtgtccg
     2881 cgagcgcggt cccgccggca gcggagatct cggccacgac ctcgaaggcc gggccggcat
     2941 ccacaccgtc gccctccatc gacgagccga ggtcgttgac gactacctct gcgcccagcg
     3001 ccgcgagcag gtgcgcgtac gcacgcccga tgccccgacc cgcgcccgtg acgaccgcta
     3061 cccgtccgtc gaatctgtgc tcgctcacct ctcccccttg tcgatgtacc ttcgggcccg
     3121 atgccggcca cggagtccca acgcagcgag aagtggaagg cgcgtcgcga ccagatcgtc
     3181 gacacgtcgg ccaaggtctt cgcgcgcggc ggctaccacg cgaccggcat cgcggagctg
     3241 tgcaccgtca acgggctcgg caagggcgcc ttctaccact acatcggatc gaaggaagag
     3301 ctgctcgccg cgattcacga ccgcgtgatg gacgaagtga tggcgggcgc cgaccgcgtt
     3361 gccgcgagcg gcgccccacc ggccgcgcaa ctcgcgatgc tcggcgacga actgctcgac
     3421 gttatccgcc gctatcccga ccacgtgtgg gtgttcctgc acgagttccc g
//
LOCUS       DASUGA010000413         2377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1122928, whole genome shotgun sequence.
ACCESSION   DASUGA010000413 DASUGA010000000
VERSION     DASUGA010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122928"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1637)
                     /locus_tag="VFX21_08210"
     CDS             complement(39..1637)
                     /locus_tag="VFX21_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019806549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5095981.1"
                     /translation="MTARVTEAAHAVRILARAGVLRPGRPDRLLRMAGAVRHWGPGLA
                     GAFAAGAARYGDNRAIVDEYGTVTFSELDARTDALARGLRARDIGAGDTVGVLCRNSR
                     YFFDAMGALAKLGANALLLNTGFSAPQLRDVMAREQAAGLLHDDEYREIVADAGITNG
                     IVAWTTGNASETTIDALIAEHGDGATLPRPDRDGRTIILTSGTTGTPKGALQSNTSDA
                     TAGISLLERLPYHARDTIVIAAPCFHAWGLTNSLLSLLLGNTMVLERFFAPETTLAAL
                     ARNRAQVLAAVPVMLLRIADLPAEVRARYDTSMLRFVPLSGSALPGDLATRFMDAYGD
                     VLYNLYGSTEVGAVSVASPADLRAAPSTAGRPPRGTEVRLLDEGGRELEPGETGRIFV
                     RSPLAFDGYTDGRTKTVIDGFMQTGDTGHFDADGRLFVDGRDDDMIISGGENVFPGEV
                     EDVIASHPDVVEAAVVGVDDPEFGQRLKAFVVQREGTTLDADSVRTLVREQLARYKVP
                     RDVVFVAELPRTTTGKVLRRHLAD"
     gene            1685..>2377
                     /locus_tag="VFX21_08215"
     CDS             1685..>2377
                     /locus_tag="VFX21_08215"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX5095982.1"
                     /translation="MSSTMDVPVVSADASTDEVETALRSAGCVVVERLLSDAFVDAVN
                     AELDPYVAATAVGADDFAGVNTKRTGALLARSPGFREMAIHPTVTGALDRVLADHATS
                     YQLHLTQVIEIGPGSPAQLVHRDQWAFDFFPFPQGYEVECHTMWAMQDFTEENGATRV
                     IPGSNTWADKLRPTLEETVPVEMPKGSVMLYLGSLYHGGGPNRSNAPRRGVNVGYTLS
                     WLRQEENQYLACP"
BASE COUNT          398 a          778 c          814 g          387 t
ORIGIN      
        1 ggtcatgccc tgatcttcgc cgcgcacccg cgatcggatc aatcggccag atgccgcctc
       61 aggaccttgc cggtggtggt gcgcggcaac tcggcgacga agaccacgtc gcgaggcacc
      121 ttgtagcggg ccagctgctc ccggaccagc gtccgcaccg aatcggcgtc gagggtcgtt
      181 ccctcgcgtt gcacgacgaa cgccttcaga cgctggccga attccggatc gtcgaccccg
      241 acaacggcgg cttcgacgac atcgggatgg gacgcgatga catcttcgac ttcgccgggg
      301 aacacgttct cgccgcccga aatgatcatg tcgtcgtcgc ggccgtcgac gaacaggcgg
      361 ccgtcggcat cgaagtgtcc ggtgtcgccg gtctgcatga agccgtcgat gacggttttc
      421 gtgcggccgt ccgtgtagcc gtcgaaggcc aacggactgc ggacgaagat gcggccggtt
      481 tcgcccggct ccaactcgcg gccgccttcg tcgagcaacc gcacttcggt gccgcgcggc
      541 ggccggcccg cggtcgacgg cgcggcgcgc aaatcggccg gggacgccac cgaaactgcg
      601 ccgacttcgg tcgagccgta caggttgtag agcacatcgc cgtacgcgtc cataaagcgg
      661 gttgcgagat cgccggggag cgccgagccc gagagcggga cgaatcgcaa catcgacgtg
      721 tcgtagcgcg cacgaacttc ggcgggcagg tcggcgatgc gcaggagcat caccggaact
      781 gcggcgagca cctgcgcgcg gttgcgcgcg agagcggcga gtgtggtctc cggtgcgaag
      841 aagcgttcga gcaccatcgt gttccccagc agcagactga gcaacgagtt cgtcagtccc
      901 caggcgtgaa aacagggtgc cgcaatcaca atcgtgtcgc gcgcgtgata cggcaaccgt
      961 tcgagcagcg atatccctgc ggtcgcgtcc gacgtgttgc tctggagggc gcccttcggc
     1021 gttccggtcg tgccgctcgt gaggatgatc gtgcgaccgt cgcgatcggg acggggcagc
     1081 gtcgcgccgt cgccgtgctc ggcaatcaac gcatcgatcg tcgtttcgga cgcgttgccg
     1141 gtcgtccacg cgacgatgcc gttcgtaatt ccggcgtcgg ccacgatctc gcggtactcg
     1201 tcgtcgtgca gcagcccggc ggcctgctct cgcgccatca catcgcgcag ctgcggcgcc
     1261 gaaaagcctg tattcagcaa cagcgcgttg gcgccgagtt tcgcgagcgc tcccatcgcg
     1321 tcgaagaagt agcgactatt ccggcagagc acaccgaccg tgtcgccggc gccgatgtcg
     1381 cgggcgcgca acccgcgggc gagcgcatcg gtgcgagcgt cgagttcgct aaaggtgacg
     1441 gtgccgtact cgtcgacgat cgcacggttg tcgccgtagc gcgccgcgcc ggcggcgaac
     1501 gcgcccgcca aacccggacc ccaatggcgc acggcgccgg ccatacgcag caggcggtcg
     1561 ggccgacccg gccggagtac gccggcgcgg gcgaggatcc gcacggcgtg ggctgcttcg
     1621 gtgacgcgcg cagtcacggg aggcacagtc gtgaacctta cgagatggcg cgtatcgttg
     1681 cgccatgtcg tcaacgatgg atgtcccggt cgtttcggcg gacgcgagta ccgacgaggt
     1741 cgaaactgcg ttgcgtagcg cgggctgcgt tgtggtcgaa cgactgcttt ccgacgcgtt
     1801 cgtcgacgcg gtgaacgctg aactcgaccc gtatgtcgcg gcaaccgcgg tgggtgccga
     1861 cgactttgcg ggcgtgaaca cgaagcggac cggtgcgctc ctcgcgcgat cgccggggtt
     1921 tcgagagatg gcaatccatc cgacggtcac aggcgcgctc gaccgggtgc tggccgacca
     1981 cgcgacgagc taccagctcc acctcacaca agtcatcgaa atcggtccgg ggtcgcccgc
     2041 gcagctcgtg catcgcgacc agtgggcgtt cgacttcttc ccgttcccgc aaggctacga
     2101 agtcgagtgt cacacgatgt gggccatgca ggacttcacc gaggagaatg gcgcgacccg
     2161 cgtgatcccc ggcagcaata cctgggccga caagttgcgg ccgacgctcg aggaaaccgt
     2221 tcccgtcgag atgccgaagg gttcggtaat gctgtatctc ggttcgctgt accacggcgg
     2281 cggaccgaat cggtcgaacg cgccgcgccg cggcgtcaat gtgggttaca cgctttcatg
     2341 gttgcgccaa gaggagaacc agtacctcgc gtgtccg
//
LOCUS       DASUGA010000414         3854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_853100, whole genome shotgun sequence.
ACCESSION   DASUGA010000414 DASUGA010000000
VERSION     DASUGA010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853100"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFX21_08220"
     CDS             <1..522
                     /locus_tag="VFX21_08220"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5095983.1"
                     /translation="GSHLVDQIRCWLGEFQHVSAALPTVADRIDAAEDSFVVRATLAG
                     GVEGVLHQTAASWIPRPMGVTAVAGTMGTLTTDGNRVTLSDRDGERTLVVDDDLRLPN
                     AAESDDPRQRFTHLELGPYTRLCEALRDRGKSTVPVPTFADGVAAMEVLDAIRVSAQL
                     GGAQVPVAHQTEP"
     gene            complement(504..1106)
                     /locus_tag="VFX21_08225"
     CDS             complement(504..1106)
                     /locus_tag="VFX21_08225"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX5095984.1"
                     /translation="MNGDAFRGLVTRQRACREFSDDPVGDDDIERCLAAATYAPSAEN
                     KQPWEFVVVRDAARRAAIGELTRRAWDAGGRAFSEGRLPQHLLQEVDRGATGGVAAAP
                     VLVVVAADTERGLPQTVPSSIFPAIQNLLLMATALGLGSALTTLSVGYAAELQALCSL
                     PTTVVPVAVIPLGYPARPLGRPRRDDFRGHTHREAYGSVW"
     gene            complement(1096..1749)
                     /locus_tag="VFX21_08230"
     CDS             complement(1096..1749)
                     /locus_tag="VFX21_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5095985.1"
                     /translation="MEDRITPGLYLEMTEQPLDEYVAERVPAVLRQDGVHRATWWRNV
                     HRDRDDLPRVLPEFGHLGVYEVDDSFIAPATPAGVTGHHFRHCPRPGQGIVTGRPTIG
                     LSLVLISPKDPLDAQALRDWADFVHIRHIAEVAVPGYAMITPYENVTGGDPHFMHFYE
                     MDTDDPERAFKSMTPLVMKRIGDASTDAFKRWARHPALRIMYVNSFARVGASDARER"
     gene            complement(1759..2601)
                     /locus_tag="VFX21_08235"
     CDS             complement(1759..2601)
                     /locus_tag="VFX21_08235"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX5095986.1"
                     /translation="MGDLAADTAVTGGDGRYTCALSRDWEIWGPNGGYMAAIALRAAG
                     EHSRFERPASIVGHFLGVAEFDTVDIEVTILRAAKRAESARVSIKQHGQPMFDAMVWS
                     VGDVEGFEHDFTEMPRVPDPDELPTNAERMAKKGEPEFTPYVFWKNFEQKIPDDKWID
                     DWESRPPRAAEYSQWYRYVPVDSFADPWVDACRLLILVDTLGWPAAGQPYPQGKYMAP
                     SIDLLCAFHRDASHSPWLYAQALAPSASAGIIGCESRVWTREGALAAVGMSQLLCRPA
                     PWAQ"
     gene            complement(2601..3074)
                     /locus_tag="VFX21_08240"
     CDS             complement(2601..3074)
                     /locus_tag="VFX21_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005515404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5095987.1"
                     /translation="MRIDIPDGSHDPEVRMWALRPEMGMAAGAMSKAIYEQSIVPVRE
                     RELARMRIAQINGCMICQQWRKTPGGAEAMTEDDYAGVEHWRTHDGYTDRERLAIEYA
                     ERFALDHDNLDDDFWARMRDAYSDAEVLDITVCIGGWIALGRTLHVLGIDEYCAL"
     gene            complement(3074..>3854)
                     /locus_tag="VFX21_08245"
     CDS             complement(3074..>3854)
                     /locus_tag="VFX21_08245"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HEX5095988.1"
                     /translation="VDAFEAAREAAAAGHRALVLKSHDFPTASLAWAVQQQTEGIGVF
                     GGICCDREIGGLNAAAVEVALRLGARIVWLPTLSSRQDFENGIAAQLGIPGPGLAVTD
                     DDGGLLPETLEVLDLVAEHGAVLATGHVSTAEHYAVVKEHARRAPVLLTHATEDLAGP
                     KLSAAQCRELADLGAWVELCAMTCIGALATKSVDDMVATIRAVGVERVTLGTDFGQKI
                     NPRPAAGLQTYADALFEAGLTEREIRTMACTNPCALLRLDA"
BASE COUNT          561 a         1334 c         1358 g          601 t
ORIGIN      
        1 gggtcgcacc tcgtcgacca gatccgctgc tggctgggcg agttccagca cgtcagcgcg
       61 gcgctgccga cggtcgccga ccgcatcgac gccgccgagg actcgttcgt ggtgcgcgcg
      121 acgctcgcgg gcggtgtcga aggcgtgctg caccagacgg ccgcgtcgtg gatcccgcgc
      181 ccgatgggtg tgaccgccgt cgcgggcacg atgggcacgt tgacgaccga cggcaaccgt
      241 gtcaccctct ccgatcgcga cggtgaacgc acgctcgtcg tcgacgacga cctccgccta
      301 cccaacgcgg ccgagagcga cgatccgcgc cagcggttca cgcacctcga gctcgggcca
      361 tacacgcgcc tgtgcgaggc gctgcgcgac cggggcaaga gcaccgtgcc ggtgccgacc
      421 ttcgcggacg gcgtggccgc gatggaagtg ctcgacgcga tccgtgtgtc tgcgcaactg
      481 ggtggcgcgc aggttcccgt cgctcaccaa accgagccgt aagcctcgcg gtgcgtatgg
      541 ccgcggaagt cgtcgcggcg cggccggccg agcggccggg ccggatagcc gagcgggatg
      601 accgccaccg gtacgacagt tgtcgggagc gagcacagcg cctgcagctc ggcggcatac
      661 ccgacgctca gcgtcgtcag cgcgctgccc aagcccaacg cggtcgccat caacaagagg
      721 ttctggatcg ccgggaagat cgacgacggc accgtctggg ggaggccccg ttccgtatcg
      781 gcggccacga cgaccaggac cggtgcggcg gcgacgccgc cggttgcacc ccggtcgacc
      841 tcctgcagca ggtgctgcgg gaggcggcct tcggagaagg cgcggccgcc cgcgtcccac
      901 gcgcggcgcg tcagctcgcc gatcgcggcg cgccgtgcgg cgtcgcgcac cacgacgaac
      961 tcccagggct gcttgttctc ggcgctgggg gcgtaggtcg cggccgcgag gcagcgttcg
     1021 atgtcgtcgt ccccgaccgg gtcgtcggag aactcgcggc acgcgcgctg gcgcgtcacc
     1081 aggccgcgga acgcgtcacc gttcacgtgc atcactcgcg ccgacccgtg cgaacgagtt
     1141 cacgtacatg atgcgcaacg ccgggtggcg tgcccagcgc ttgaacgcgt cggtcgaggc
     1201 atcgccgatc cgcttcatga ccaacggtgt catcgacttg aacgcgcgct ccggatcgtc
     1261 ggtgtccatc tcgtagaagt gcatgaagtg cggatcaccg cccgtcacgt tctcgtacgg
     1321 cgtgatcatc gcgtagcccg ggaccgcgac ctccgcgatg tggcggatgt gcacgaagtc
     1381 cgcccagtcg cgcagggctt gcgcgtcgag cggatccttc ggagagatca gtaccagcga
     1441 caggccgatc gtcggccgcc ccgtgacgat cccctggccg ggccgcgggc agtggcggaa
     1501 gtggtggccg gtgacgccgg cgggcgtcgc gggcgcgatg aaggagtcgt cgacctcgta
     1561 cacgccgagg tggccgaact cgggcaggac gcgagggaga tcgtcgcggt cgcggtgcac
     1621 gttgcgccac caggttgcgc ggtgcacgcc gtcttgacgc aacacagcgg gaacgcgttc
     1681 ggcgacgtac tcgtcgagcg gttgttcggt catctccaga tagagcccgg gcgtgatgcg
     1741 gtcttccatt gcgatgactt actgcgccca gggggcgggc cggcacaaca gttggctcat
     1801 cccgaccgcg gcgagggcgc cttcgcgcgt ccacacgcgg ctttcgcagc cgatgatccc
     1861 ggccgatgcg cttggcgcga gcgcctgcgc gtacagccac ggcgaatgcg acgcgtcgcg
     1921 gtggaacgcg cacaagaggt cgatgctcgg cgccatgtac ttgccctgtg ggtacggctg
     1981 accggccgcg ggccaaccga gggtgtcgac gagaatcagg agccggcacg cgtcgaccca
     2041 cggatccgcg aacgagtcga cggggacgta gcggtaccac tggctgtact ccgcggcgcg
     2101 cggcggccgc gactcccagt cgtcgatcca cttgtcgtcc gggatcttct gctcgaagtt
     2161 cttccagaac acgtacggcg tgaactccgg ctcgcccttc ttcgccatac gttcggcgtt
     2221 ggtcggcaac tcgtcggggt ccggtacccg tggcatctcg gtgaagtcgt gctcgaagcc
     2281 ttcgacatcg ccgacgctcc acaccatggc gtcgaacatc ggttggccgt gctgcttgat
     2341 cgacacgcgt gccgactcgg cccgcttcgc ggcgcgcaga atcgtgacct cgatgtcgac
     2401 ggtgtcgaac tcggccacac cgaggaagtg tccgacgatg ctcgcgggcc gctcgaaccg
     2461 ggagtgctcg cccgccgccc gcaacgcgat agcggccatg tagccaccat tcgggcccca
     2521 gatctcccag tcgcgcgaaa gggcgcacgt gtagcgaccg tcgccgccgg tgaccgcggt
     2581 gtcggcggcg aggtctccca tcagagcgcg cagtactcgt cgatgccgag cacgtgcagc
     2641 gtgcggccca gtgcgatcca accgccgatg cagacggtga tgtcgagcac ttcggcgtcg
     2701 ctgtatgcat ctcgcatgcg agcccagaag tcgtcgtcga gattgtcgtg gtcgagcgcg
     2761 aaccgctcgg cgtactcgat cgcgagccgt tcacggtcgg tgtagccgtc gtgcgtgcgc
     2821 cagtgctcga cgccggcgta gtcgtcctcg gtcatcgcct cggcaccgcc gggcgtcttg
     2881 cgccactgct gacagatcat gcagccgttg atctgcgcga tccgcatgcg cgcgagctcg
     2941 cgttcgcgca cgggcacgat gctctgttcg tagatggcct tgctcatcgc gccggccgcc
     3001 atgcccatct cggggcgcag cgcccacatg cgcacttcgg gatcgtggct gccgtcgggg
     3061 atgtcgatgc gcatcacgcg tccaatcgca gaagggcgca ggggttcgtg cacgccatcg
     3121 tacggatctc gcgctcggtg agcccagctt cgaacagcgc gtccgcgtag gtctggagcc
     3181 cggccgccgg gcgcgggttg atcttctggc cgaagtcggt cccgagcgtg acccgctcga
     3241 caccgacggc ccggatcgtg gcgaccatgt cgtcgacgct cttggtcgcg agcgcgccga
     3301 tgcaggtcat cgcacacagc tcgacccagg cgccgaggtc ggccagctcc cggcactgcg
     3361 cggcgctgag cttcggcccc gcgaggtctt cggtcgcatg cgtcagcaac accggcgccc
     3421 ggcgcgcgtg ctccttgacg acggcgtagt gctcggccgt gctcacgtgc ccggtggcga
     3481 gcaccgcgcc gtgctcggcg acgaggtcga gcacctcgag ggtctcgggc agcagtccgc
     3541 cgtcgtcgtc ggtgaccgcg aggcccgggc ccgggatgcc gagctgcgcg gcgatgccgt
     3601 tctcgaagtc ctggcggctc gacagcgtcg ggagccacac gatgcgcgcg ccgagccgca
     3661 gtgcgacctc gaccgcggcc gcattcaggc cgccgatctc gcggtcgcag cagatgccgc
     3721 cgaagacgcc gatgccctcg gtttgttgtt gcacggccca tgcgagcgac gcggtgggga
     3781 agtcgtgact cttcaggacg agcgcgcggt gaccggccgc ggcggcttct cgcgccgctt
     3841 cgaacgcgtc gacc
//
LOCUS       DASUGA010000415         3367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_280507, whole genome shotgun sequence.
ACCESSION   DASUGA010000415 DASUGA010000000
VERSION     DASUGA010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280507"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1481)
                     /locus_tag="VFX21_08250"
     CDS             complement(6..1481)
                     /locus_tag="VFX21_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011605243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5095989.1"
                     /translation="MASDLKRDRLYIGGEWVDAAGDGHIDVVNPATEEVIAQVAEGSV
                     ADVDRAVAAARKAYEEGPWRRMSPRERSDALVRFVQTVENKRAELVDLIIAEAGAARS
                     IAGALQFDTPMRYSMWFAERVASFPFMDPMPVNVTARGMGQGVIIKEPIGVVAAITPF
                     NFPLYLNLVKIVPALAMGNSVVLKPSPLTPLEAFALGDIANEAGLPPGVLNVVTGDIA
                     ASEHLTTHPMVDMVSFTGSDDVGKKIMGQAAPGLKKVLLELGGKSPNIVFNGSDVAKF
                     SFSAAFGFTIHAGQGCALPTRILAERAIYDEVVDGMTAALQKTTVGDPTDVKTGMGPL
                     IRETQRARVERYVETGIAEGARVATGGRRPDGMTRGFFYEPTLFADVKSSMAVAQDEI
                     FGPVGVVIPFDGEEEAIAIANDSRFGLAASVWHPDPVRAFEIAKEIQSGTVSINGGGG
                     GPNLWGPFGGYKQSGIGREFGDYGLLEYAQLKTVAWSAGRP"
     gene            1587..2861
                     /locus_tag="VFX21_08255"
     CDS             1587..2861
                     /locus_tag="VFX21_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006383532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5095990.1"
                     /translation="MHDTTAFDAVDFFRARDRWRDPYPYYEYLRSHGPVWREPHHGVV
                     MVTGFEETMSVYNDHDRWSSCNTVAGPWARFPVPLVGDDIGEIIEQYRDQLPFSDQLP
                     SFDQPKHTAHRGLLMRLITPKRLKENEEFMWRLADRQLDEFVARGACDFVRDYANPFT
                     LLVVADLLGVPEEDHETFRAELQGDERPTSKRGEMMHKPLEFLYERFTRYIEERRREP
                     RDDVMTELALAPFPDGSLPDIHDVMLIASNLFAAGGETTARLLTQMMRYTAESPELQQ
                     LLRDDREKIPVLAEESVRLECPIQGEFRLARTATTIAGVDLPAGSTAWLINGAANRDP
                     RQFDHPNELRLDRVNGRQHLGFGFGIHTCAGAPLARAEARVSVERILQRVDDIRISDA
                     QHGPAGDRHWEYTPIYMLRGLEHLYLEFTPRG"
     gene            2854..3219
                     /locus_tag="VFX21_08260"
     CDS             2854..3219
                     /locus_tag="VFX21_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019930796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5095991.1"
                     /translation="MDDAIVQRLRRHWEDGWNTGDLDLIMAPFADDVVFASPGIAMMT
                     HDPSRTMIEGAAALRDYVDNALRHTRDVHYELHATHVGTDSVVLSYTCGFPDGPQKAG
                     ADLMRVDATGQVVEWRCHY"
BASE COUNT          547 a         1183 c         1109 g          528 t
ORIGIN      
        1 cgacgctacg gccggcccgc cgaccatgcc accgtcttga gctgcgcgta ctcgaggagt
       61 ccgtagtcgc cgaactcgcg accgatgccc gactgcttgt aaccgccgaa cgggccccac
      121 aggttcgggc cgccaccacc gccgttgatg ctcacggtgc ccgactggat ctccttcgcg
      181 atctcgaacg cccgcaccgg gtcgggatgc cacaccgacg ccgcgaggcc gaagcgagag
      241 tcgttcgcga tcgcgatcgc ctcctcttca ccgtcgaacg ggatcacgac gccgaccgga
      301 ccgaagatct cgtcctgcgc gacggccatc gagctcttca cgtcggcgaa caacgtcggc
      361 tcgtagaaga agccgcgcgt catgccgtcg ggacggcggc cgccggtcgc gacacgcgcg
      421 ccttcggcga taccggtctc gacgtagcgc tcgacgcgcg cccgttgcgt ctcgcggatc
      481 aacgggccca tgccggtctt cacgtcggtc gggtcgccga ccgtcgtctt ctgcagcgcg
      541 gccgtcatgc cgtcgacgac ctcgtcgtag atcgcccgtt ccgcgaggat gcgcgtcggc
      601 agcgcgcagc cctggccggc gtggatcgtg aagccgaacg cggccgagaa cgagaatttg
      661 gcgacgtcgg agccgttgaa cacgatgttg ggcgacttcc cgcccagctc cagcagcact
      721 ttcttcaggc ccggcgcggc ctggcccatg atcttcttcc cgacgtcgtc ggagcccgtg
      781 aagctgacca tgtcgaccat cggatgcgtc gtgaggtgct cgctcgcagc gatgtcgccg
      841 gtcacgacgt tgagcacacc gggcgggagc cccgcctcgt tcgcgatatc gccgagcgcg
      901 aaggcctcca gcggcgtcaa cggcgacggc ttcaacacga cgctgttgcc catcgcgagc
      961 gcgggcacga tcttcacgag gttgaggtac agcgggaagt tgaacggcgt gattgcggcg
     1021 acgacaccga tcggttcttt gatgatgacg ccttgcccca tgccgcgggc ggtgacgttg
     1081 accggcatcg ggtccatgaa cgggaagctg gcgacgcgct ccgcgaacca catcgagtac
     1141 cgcatcgggg tgtcgaactg gagcgcaccc gcgatcgacc gcgccgcgcc cgcctccgcg
     1201 atgatcaggt cgacgagctc ggcgcgcttg ttctcgacgg tctgcacgaa gcgcacgagc
     1261 gcatcggacc gttcgcgcgg cgacatgcgc cgccacggtc cttcttcgta cgccttgcgc
     1321 gccgccgcaa ccgcgcggtc gacgtcggcg acggagcctt ccgcgacctg ggcgatgact
     1381 tcctcggtgg ccgggttgac gacgtcgatg tgcccgtcgc cggccgcgtc gacccactcg
     1441 ccgccgatgt agaggcggtc gcgcttcagg tcgctggcca tggtgctcgc tctccggtcg
     1501 gggtcgatcc gcccgggaac ctatgtgaga acgtcgctct ccgcaagcct tccccttggc
     1561 ggaaatggcg ttatacgatc accggcgtgc acgacaccac cgccttcgac gcggtcgact
     1621 tcttccgggc ccgcgaccga tggcgcgacc cctacccgta ctacgagtac ctccgctcgc
     1681 acggccccgt gtggcgcgag ccgcaccacg gggtggtgat ggtcaccggc ttcgaggaga
     1741 caatgtcggt gtacaacgac cacgaccgct ggtcgtcgtg caacacggtg gccggaccgt
     1801 gggcgcgctt cccggtgccg ctcgtcggcg acgacatcgg cgagatcatc gagcagtacc
     1861 gcgaccagct gccgttcagc gaccaactgc cttcgttcga ccagccgaag cacaccgcgc
     1921 accgcgggct gctgatgcgg ttgatcacac ccaagcgcct gaaggagaac gaggaattca
     1981 tgtggcggct cgccgaccgt cagctggacg agttcgtcgc gcgcggcgcg tgcgacttcg
     2041 tacgtgacta tgcgaacccg ttcacgttgc tcgtcgtcgc cgacctgctc ggtgtacccg
     2101 aagaagacca cgagacgttc cgcgccgaat tgcagggtga cgagcgcccg acgagcaagc
     2161 gcggcgagat gatgcacaag ccgttggagt tcctctacga gcgcttcacg cgctacatcg
     2221 aggagcgccg gcgcgagccg cgcgacgacg tgatgacgga gctcgcgctc gcgccgttcc
     2281 ccgacggttc gctgcccgac atccacgacg tgatgttgat cgcgagcaac ctgttcgcgg
     2341 cgggcggcga gaccaccgcg cggctgctca cgcagatgat gcgctacacg gccgaatcgc
     2401 cggagctgca gcaactgctc cgcgacgacc gggagaagat cccggtgctc gccgaggaat
     2461 ccgtgcgcct cgagtgcccg atccaagggg aattccggct ggcacggacg gcgaccacga
     2521 tcgccggcgt cgacctgccc gcgggcagca cggcgtggct gatcaacggc gcggccaatc
     2581 gtgacccgcg ccagttcgac cacccgaacg aactgcgcct cgatcgcgtc aacggccgcc
     2641 agcacctcgg gttcgggttc ggcatccaca cgtgcgcggg tgcgccgctc gcgcgcgccg
     2701 aagcgcgcgt cagcgtcgaa cgcatcctgc aacgcgtcga cgacatccgg atctccgacg
     2761 cgcagcacgg tccggccggt gaccggcact gggagtacac gccgatctac atgctgcgcg
     2821 gcctcgagca cctgtacctg gagttcacgc cgcgtggatg acgcgatcgt gcagcgactc
     2881 cggcgccatt gggaagacgg ctggaacacc ggtgacctcg acctgatcat ggcgccgttc
     2941 gccgacgacg tcgtgttcgc gtcgcccggc atcgcgatga tgacgcatga tccgtcgcgc
     3001 acgatgatcg agggtgccgc cgcactgcgc gactacgtcg acaacgcgct gcggcacacg
     3061 cgcgacgttc actacgagtt gcacgcgacg catgtcggca ccgacagcgt cgtgctctcg
     3121 tacacatgcg gatttcccga cggtccccag aaggcaggtg cggatctgat gcgcgtcgac
     3181 gcgacgggcc aggtcgtcga gtggcggtgc cactattgag cgcgaccgaa ccggcctggc
     3241 gcgagcgggc ggtctcccgg agcgtcgccg ccgcccgctt gcgggccgaa gaacgtgtgc
     3301 agcgcttcct cgacgccggc ttcgcgctcg tcgacgagaa gggcaccacc gacttcacga
     3361 tccagga
//
LOCUS       DASUGA010000416         2491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_763370, whole genome shotgun sequence.
ACCESSION   DASUGA010000416 DASUGA010000000
VERSION     DASUGA010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763370"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /gene="moaA"
                     /locus_tag="VFX21_08265"
     CDS             complement(<1..977)
                     /gene="moaA"
                     /locus_tag="VFX21_08265"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010640417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX5095992.1"
                     /translation="MRARPIPLIDPFDRRVKDLRISITDRCNFRCAYCMPEEGMDWLP
                     RTELLTYEEIGRIARVCVERYGFEAIRLTGGEPLIRAHITRLIQLLAPLGVDIAMTTN
                     GVKLGESAHDLKAAGLKRINVSLDTLQRERFVALTKRDELDRVLKGIAAALDAGLDPV
                     KVNAVVMRGINDDEVVDLARFGRENNVGVRFIEFMPLDAQGEWSNDKVVPAHEIIERI
                     DAVFPLERIAPDHVEPAERSLFRDGVGDVGVIASVTEPFCDNCDRVRVTAEGQFRTCL
                     FALEETDLRSVLRAGIGLDEPDLDDRLAGAIEAAVATKWAGHRIGQVDFV"
     gene            complement(1525..>2491)
                     /gene="glp"
                     /locus_tag="VFX21_08270"
     CDS             complement(1525..>2491)
                     /gene="glp"
                     /locus_tag="VFX21_08270"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX5095993.1"
                     /translation="APTTAVGPGEAIRIMTGAPIPDGADAVVMVERTETVGDQVTVEA
                     AKLGEHIRPAGGDVEPGTLVFTRGTLLTPAHLGVLASLDVREVECHPRPRVCVVSTGD
                     ELVATGRLEPGQIRDSNRPMLLALLEESGLDAVDGGIAGDDEAVITAAVERAADGCDA
                     LLTSGAVSVGDYDFVKLVLERVAKERGGVSLWAQVAIKPAKPLAFATLGSVPAVGLPG
                     NPVSSRVSFELFARPALRKLAGRTDVLPEPVTAIAAQALDRRSDAKLHLDRVRVWIED
                     GRYRCERAGFQASNVLSGMAAANGLALIPDGPGIEAGQEVKVLLL"
BASE COUNT          415 a          853 c          811 g          412 t
ORIGIN      
        1 acgaaatcga cctgcccgat gcggtgcccg gcccacttcg tcgcgaccgc ggcttcgatc
       61 gcaccggcga gccggtcgtc gagatcgggc tcgtcgagac cgatccccgc gcgcaggact
      121 gaccgcagat cggtttcctc gagcgcgaac aaacacgtgc ggaactgacc ttcggcggtg
      181 acgcgcacgc gatcgcagtt gtcgcagaac ggttcggtca cgctcgcgat cacaccgacg
      241 tcgccaacgc cgtcgcggaa caaggagcgc tcggccggct ccacgtgatc gggcgcgatg
      301 cgctcgagcg gaaacacggc gtcgatccgt tcgatgattt cgtgcgcggg gacgaccttg
      361 tcgttgctcc actcaccttg cgcatcgagc ggcatgaact cgatgaagcg cacaccgacg
      421 ttgttttccc ggccgaatcg agccaagtcg acaacttcgt cgtcgttgat accgcgcatc
      481 acgactgcgt tgaccttgac gggatcgagg cccgcgtcga gcgccgcagc gatgcccttg
      541 agcacgcggt cgagctcgtc gcgtttggtc agcgcaacga agcgctcgcg ctgcaatgtg
      601 tcgagcgaga cgttgatgcg tttgagaccg gccgctttca gatcgtgcgc actctcgccg
      661 agcttcacgc cgttcgttgt catggcgatg tcgacgccga gcggcgcgag cagctggatc
      721 aggcgcgtga tgtgcgcgcg aatcaacggc tcgccaccgg tcaggcggat tgcctcgaac
      781 ccataccgct cgacgcagac gcgcgcgatg cgaccaattt cttcgtaggt gaggagttcg
      841 gtgcgcggca gccagtccat gccctcttcg ggcatgcagt aggcgcagcg aaagttgcat
      901 cgatcagtga tcgagatgcg caggtccttc acgcgacgat cgaagggatc gatcaacgga
      961 atcggacgcg ctcgcacagc gcccaggata ggaaagccgt ctgcggggtc aatcctccgc
     1021 agtggctccc agggtcgggt tcgctctagc tccccgccgg cgcagcgacc aacttcagcc
     1081 gacagatctt tgcttccctg ggtgtgcagc gtcgcgccag cccaacgcgc cattgcacct
     1141 tgccactcgg tgcagcctcc cgctgctggt gtttcgcccg cccgggcgac ctcgtagcgt
     1201 ggtcatctaa gcacgactcg cggcatttca caagtgtttt ccccacccgt gaccttcgcc
     1261 acaacaccac gcagagtatt gatccaatca ctcctagtcc ggatactgcg cctagcggat
     1321 ctgctcgatg agcggaacgg gcactgctca acggagagcg ggaccacata gcgggcaggc
     1381 gtcgaaatcc ccccttcggc gcctgctcaa tgtggacttc cccgggctga tctgcgcagt
     1441 ttccaaagga gtggggcgga gcatgcgaac cttcccgggc ggaggttcgc atgattcgcg
     1501 tctggaccca attccgcgcg tgccctacag caggagcacc ttgacctctt ggccggcttc
     1561 gatgccgggg ccgtcgggga tgagggcgag gccgttggcg gcggccatgc ccgacaaaac
     1621 gttgctcgcc tggaacccgg ccctctcgca gcgataccgg ccgtcctcga tccacacccg
     1681 gacccggtcg agatgcagtt tggcgtccga gcgccggtcg agcgcctggg ccgcaatggc
     1741 cgtgaccggt tcgggcagca catcggtgcg gccggcgagc ttgcggagcg cgggccgggc
     1801 gaacagctcg aagctgacgc gtgaggagac gggattgccg gggagcccaa cggcgggcac
     1861 cgatcccaac gtcgcgaagg ccaatggctt ggcgggcttg atcgcaacct gggcccagag
     1921 cgagacgccg ccccgctcct tggccacgcg ctcgaggacg agcttcacga agtcgtagtc
     1981 gccgaccgat accgcaccgc ttgtgagcaa ggcgtcgcag ccgtcggccg ctcgctccac
     2041 ggccgcggtg atcaccgctt cgtcgtcgcc cgcgatcccg ccgtcgacgg cgtcgaggcc
     2101 cgactcctcg agcagcgcca gcaacatggg ccggttggag tcgcgaatct ggcctggctc
     2161 caggcgaccg gtcgcgacca gctcgtcgcc ggtggacacc acgcacacac gcggccgcgg
     2221 atggcactcg acctcgcgga cgtcgaggct ggcgagcacc ccgaggtgcg ccggcgtcag
     2281 caacgtgccg cgggtgaaca caagcgtgcc cggctcgacg tcgccgcccg cgggccggat
     2341 gtgctcaccg agcttggcgg cctcgaccgt gacctgatcg ccgaccgttt ctgtgcgttc
     2401 gaccatcacg acggcgtcgg caccatcggg aatcggcgcg cccgtcatga tgcggatcgc
     2461 ttcgcccggc cccaccgcgg tcgtcggcgc c
//
LOCUS       DASUGA010000417         2230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_575182, whole genome shotgun sequence.
ACCESSION   DASUGA010000417 DASUGA010000000
VERSION     DASUGA010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575182"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..>2230
                     /locus_tag="VFX21_08275"
     CDS             300..>2230
                     /locus_tag="VFX21_08275"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5095994.1"
                     /translation="MLGGRRGESVSGACGRPATRRRTAAALVLVLATACSSSDDHDAR
                     PEPAPSSTTVRGETNAPVETDWTVYGHDLANTRLSAGETRITTATVGGLEEEWSVDDL
                     VGVTGTPVVADGVAYFGDWTGKVHAVDTGSGTDRWAAQVGGFVVGSPAVDGDAIFVAS
                     GKTLFRLDRATGTIGWQVDVNEHDLAQISASPVVVGDVVLQGVASAEVVMAKDEYTFR
                     GSIGAYDVATGAEKWRFYTTAGDATDGPGAGVWSTPAVDVDRGVLYVGSGNAYAEPTG
                     PLADSILAIDIETGALQWSTQFTYPDVFSGANPGGKDADVGASPNLWSVDGHDYVGAG
                     DKGGVYHALDRDTGEIAWETTLTPGSVFGGEIGSGAFVDDRLVVVSNVGDPASNAPTN
                     VAKVFALDPETGDILWEADQFDGLIFGPVSAVPGVAFVGTNTGKLAALDTETGAERWS
                     FQAPAPVGGGASIVDGRVVWGYGFTLFDGPGQGGVLSFTVPDDTTASAARVDASASAG
                     CDATPATPGVSDRTLSFGGEDRNYQLTLPAHYDGRTPLPVVFGLHALTISYEFVPSMV
                     GFDAMQREYDFIGVAPSGRRNGDAPYWFAAPAEPNDDVEFLGALLDELENTLCVDPAR
                     VFATGMSNGAQMSSQLACRL"
BASE COUNT          341 a          749 c          791 g          349 t
ORIGIN      
        1 cgtcgacaac gtcccggtga cgcgcgtctt cgtcggtcat gacgattgcc gtacccgatc
       61 gcgtgtcggc agacggcgcg ttgtctgaag gaattcccga aggaaatcgc gattgcccgt
      121 accgaatcac aacgatgcga cgttcacgtc attgatcgca cggatgcatc gccgcaagga
      181 tgcgcgaagt cgcagggata gcgacgctgg ccgcggcccg caccattcac atgggcgggc
      241 cgcgtgtcgc gtctgcggtc ctttcagacg ggctgtcgcc gtgagtagcg taccgagacg
      301 tgctcggcgg ccggcgtggg gagtccgttt ccggcgcgtg cggcaggccg gcgacccgac
      361 ggcggacggc ggccgcgctg gtcctcgtgc tcgcgacggc gtgcagctcg tccgacgacc
      421 acgacgcccg cccggagccc gcaccgtcgt cgaccacggt acgcggagaa acgaacgcgc
      481 cggtcgagac cgattggacg gtctacgggc acgacctcgc caacacccgt ctgagcgcgg
      541 gtgagacgcg catcaccacg gcaaccgtcg gtgggctcga ggaggagtgg tcggtcgacg
      601 atctcgtcgg cgtgaccggt actccggtcg tcgcggacgg tgttgcctac ttcggcgact
      661 ggacgggcaa ggtgcacgcc gtcgacaccg ggtccggcac ggatcgttgg gccgcacaag
      721 tcggcggatt cgtcgtcgga tcgccggccg tcgacggcga cgccatcttc gtcgcgagtg
      781 gcaagacgct gttccgcctc gaccgcgcga ccggaacgat cggctggcag gtcgacgtga
      841 acgagcacga cctcgcgcag atctcggcgt caccggtcgt cgtgggtgac gtcgtgctcc
      901 agggcgtcgc cagcgccgaa gtcgtcatgg cgaaggacga gtacacgttc cgcggctcga
      961 tcggcgccta cgacgtcgcg acgggcgccg agaagtggcg cttctatacg accgcgggcg
     1021 acgcgaccga cggccccggc gcgggcgtgt ggtcgactcc cgccgtcgac gtcgaccgcg
     1081 gtgtcttgta cgtggggagc ggcaacgcgt acgccgaacc caccggaccc ctcgcggact
     1141 cgatcctcgc gatcgacatc gagaccggcg cgttgcagtg gtcgacgcag ttcacgtatc
     1201 cggacgtgtt cagcggcgcg aatccgggcg gcaaggacgc ggacgtgggc gcgtcgccga
     1261 atctgtggag cgtcgacggt cacgactacg tcggcgcggg cgacaaaggc ggcgtgtacc
     1321 acgcgttgga ccgcgatacc ggcgagatcg cgtgggagac gaccctgaca ccgggcagcg
     1381 tgttcggcgg tgagatcggt tcgggcgcgt tcgtcgacga ccggctcgtg gtcgtctcca
     1441 acgtcggcga tcccgcgtcg aacgcgccga cgaacgtcgc caaggtcttc gcgctcgatc
     1501 ccgagactgg cgacatcctc tgggaagcag atcagttcga cgggttgatc ttcgggcccg
     1561 tcagcgcggt gccgggcgtc gcgttcgtcg gcaccaacac cgggaagctg gcggccctcg
     1621 acaccgagac cggcgccgag cggtggtcgt tccaagcgcc ggccccagtc ggcggcggcg
     1681 cgtcgatcgt cgatggtcgc gtcgtgtggg gatacggatt cacgctgttc gacggtccgg
     1741 gccagggcgg cgtgctcagc ttcacggtgc cggacgacac cactgcgagc gcggctcgtg
     1801 tggacgcgtc ggcgtcggcg ggctgcgacg cgacgccggc cacgcccggc gtgtccgatc
     1861 gcacgctttc cttcggcggc gaagatcgga actaccaact cacgttgccg gcgcactacg
     1921 acggccggac gccactgccg gtcgtcttcg ggctgcacgc gctcaccatc agctacgagt
     1981 tcgtcccgtc gatggtcggc ttcgacgcaa tgcagcgcga gtacgacttc atcggcgtcg
     2041 caccatccgg gcggcgcaac ggcgacgctc cctactggtt cgcggcgccc gccgaaccga
     2101 acgacgacgt cgagttcctc ggtgcactcc tcgacgagct cgagaacacg ttgtgcgtcg
     2161 atcccgcgcg cgtcttcgcg accggcatgt cgaacggcgc gcagatgtcg tcgcagctcg
     2221 cgtgccgact
//
LOCUS       DASUGA010000418         2476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1841660, whole genome shotgun sequence.
ACCESSION   DASUGA010000418 DASUGA010000000
VERSION     DASUGA010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1841660"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1347)
                     /locus_tag="VFX21_08280"
     CDS             complement(85..1347)
                     /locus_tag="VFX21_08280"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5095995.1"
                     /translation="MSNNDRYLIISADCHAGLPCEEYRPYLDPKYSEAFEEYLAERQA
                     MRAQRMADSGDYITHWEEENAEGLTGAYDPAQRDRELDGDGVAGEVLFPDADAITGNA
                     SPPFGAGLSAADITDPELAFAGARAHNRFLAELCAHSPERRAGVALVPITHDVPRAVA
                     EIEWAAENGLRGGILIPTMWREHAPYVDPVYEPVWAACAANGMTVHTHSGTAPQEDYQ
                     NNAGVYLAEVVWWAVRPMWFLLFGGAFERHPDLQYCVTESAAYWAPDLMWKWDTYLGG
                     GHTTKKMVAQLAGKVSRLPSEYFGTNVFIGASTMSREEIRRRYAIGTDTVMWGNDYPH
                     PEGTWPHTIAKLKETFHDVPVEDARRMLGTNAASVYRFDVDALAPLADRIGPMPTDLG
                     QDPDRAVDPAEVAAGRWWKEGLVPPGSV"
     gene            complement(1352..2140)
                     /locus_tag="VFX21_08285"
     CDS             complement(1352..2140)
                     /locus_tag="VFX21_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HEX5095996.1"
                     /translation="MAQVRYAAKPLEARRNREIEATKGEIWSQALTAIYETDPDVLAS
                     VLPPPLQPPAEPLVRATITTVDMPGGYTFGAGYFAVRAAHEGVEGEYPLFMPMTTEQA
                     TVGGRETFGEPKKIGEVWVKRDGDAVQGVMARMGFTVVEITGRVVETLPPPPERTKTD
                     FYFKFLPSPDGDGFDSDPALVYCHKKERMRVLERVEGDVILKDSPLDPVADIPIVRVV
                     DLNWSERTTFQTGVIHSHVPQEWLLPFVHQRYDDLSVLGKAKKD"
     gene            2214..>2476
                     /locus_tag="VFX21_08290"
     CDS             2214..>2476
                     /locus_tag="VFX21_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5095997.1"
                     /translation="MHDLQGKVAVVTGGASGIGLAMVQRFAQEGMTAIAADVRLDAAQ
                     DAVADIDGAAAEQVDVTSYDSVAALAERVRDHHGAVHLLCNNAG"
BASE COUNT          361 a          850 c          849 g          416 t
ORIGIN      
        1 atcgacagcg cttcgacacc gctcgtctcg acgatccggc gcgcggccgc gacgatccgg
       61 tcgcgttcgc tggggtcgga gtgctcagac ggatccgggc gggacaaggc cttccttcca
      121 ccaacgaccg gccgccacct cggcgggatc gacggcgcgg tcgggatcct ggccgaggtc
      181 ggtcggcatc gggccgatcc gatcggcgag cggcgcgagc gcgtcgacgt cgaagcgata
      241 gaccgacgcc gcgttcgtgc cgagcatgcg gcgcgcgtcc tcgaccggca cgtcgtggaa
      301 cgtctccttg agcttcgcga tcgtgtgcgg ccacgtgcct tcgggatgcg ggtagtcgtt
      361 gccccacatg accgtgtcgg tgccgatcgc gtaccggcgg cggatctcct cgcggctcat
      421 cgtcgacgcc ccgatgaaca cgttcgtgcc gaagtattcg ctggggagcc gcgagacctt
      481 gcccgcgagc tgcgccacca tcttcttcgt cgtgtggccg ccaccgaggt aggtgtccca
      541 cttccacatg aggtcgggcg cccagtacgc ggcggattcg gtgacgcagt actggaggtc
      601 ggggtgtcgc tcgaacgcgc caccgaagag caggaaccac atcggccgga cggcccacca
      661 caccacttcg gcgaggtaca cacccgcgtt gttctggtag tcctcctgcg gcgcggtgcc
      721 cgagtgcgtg tgcaccgtca tgccgttcgc ggcgcacgcg gcccacaccg gttcgtacac
      781 cgggtcgacg tagggcgcgt gctcccgcca catcgtcggg atcagaattc ccccgcgcag
      841 cccgttctcg gccgcccact cgatctcggc gaccgcgcgc ggtacgtcgt gcgtgatcgg
      901 caccagcgca acgccggcgc gccgttccgg actgtgcgcg cacagctcgg cgaggaagcg
      961 gttgtgcgcg cgagcgccgg caaacgcgag ctcgggatcg gtgatatcag ccgcggacag
     1021 tcccgcgccg aacggcggcg acgcgttccc ggtgatcgcg tccgcatcgg ggaagagcac
     1081 ttcgccggca acaccgtcac cgtcgagctc gcggtcgcgt tgcgccgggt cgtacgcgcc
     1141 ggtgagccct tcggcgttct cttcttccca gtgcgtgatg tagtcgccgc tgtcggccat
     1201 ccgctgcgcg cgcattgcct ggcgttcggc gagatattcc tcgaacgcct cggagtactt
     1261 cgggtcgagg tacggccggt actcctcgca cggcagcccc gcgtggcagt cggccgagat
     1321 gatgaggtaa cggtcgttgt tgctcacggc gtcagtcctt cttcgccttg cccagtaccg
     1381 agaggtcgtc gtagcgctgg tgtacgaacg gcaacagcca ttcctgcggc acgtgcgagt
     1441 ggatcacgcc cgtctggaag gtcgtacgct cgctccaatt gaggtcgacg acgcgcacga
     1501 tgggaatgtc ggccaccggg tcgagcggcg agtccttcaa gatgacgtcg ccctcgacgc
     1561 gttcgaggac gcgcatcctc tccttcttgt ggcagtagac aagcgcggga tcgctgtcga
     1621 aaccgtcgcc gtcgggcgag ggaaggaact tgaagtagaa gtcggtcttc gtgcgctcgg
     1681 gcggcggcgg caacgtctcg acgacgcggc cggtgatctc gacgaccgtg aagcccatgc
     1741 gagccatgac cccctgcact gcgtcgccgt cgcgcttcac ccacacttcg ccgatcttct
     1801 tcggctcgcc gaacgtctcg cgcccgccga ccgttgcctg ctcggtcgtc atcggcatga
     1861 acaacgggta ctcgccctcg acgccctcgt gcgcggcgcg caccgcgaag taccccgcgc
     1921 cgaacgtgta gccgccgggc atgtcgacgg tcgtgatcgt cgcgcgcacg agcggttcgg
     1981 cgggcggctg caacggcggt ggcagcaccg acgcgagcac gtcgggatcg gtctcgtaga
     2041 tcgcggtgag cgcctgcgac cagatctcac ccttggtcgc ctcgatctcc cggttgcgtc
     2101 gggcctcgag cggcttcgcc gcgtaccgca cctgtgccat ggtggtcccc tttgcagggc
     2161 tgtgtgacgc cgttacagta acggtgttac agccgatgtc aacaggaggg tccgtgcacg
     2221 acctccaggg caaggtcgcc gtcgtgaccg gtggcgcgag cgggatcggt ctcgcgatgg
     2281 tgcagcgctt cgcgcaggag ggcatgaccg cgatcgcggc cgacgtgcgc ctcgacgcgg
     2341 cgcaggacgc ggtcgccgac atcgacggcg cggcagccga acaggtcgac gtgacgagct
     2401 acgactccgt cgccgcgctg gccgagcgcg tgcgcgacca ccacggcgcg gtgcacctgc
     2461 tctgcaacaa cgcggg
//
LOCUS       DASUGA010000419         4387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_565470, whole genome shotgun sequence.
ACCESSION   DASUGA010000419 DASUGA010000000
VERSION     DASUGA010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565470"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VFX21_08295"
     CDS             <1..278
                     /locus_tag="VFX21_08295"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ERCC4 domain-containing protein"
                     /protein_id="HEX5095998.1"
                     /translation="AGNVVDGSLLVTLGELSAAKRAAIVVEDRWADVFRLQHVAPSMV
                     SEMLAAAQVRYPNVPIVFCENRKLAQEWTYRFLGAARAYADEETFEL"
     gene            358..2892
                     /locus_tag="VFX21_08300"
     CDS             358..2892
                     /locus_tag="VFX21_08300"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5095999.1"
                     /translation="MLAAVRMVGRSERRRQRWALLALAAVVALGLGASLASFAAAYRT
                     DHAWDQYLRRAAVTDLVVNPSLPTQEFDDRLREVPHVRGMWTQDLLAAGVFNTTGPIT
                     AAEAADSDAPGEGIGSVDGRYTKADRLMVDSGRAPSGTHEVFVTETFRPRLDEIMGHR
                     VQVGDEIPVSFFAASDVSPDVSSDFDPNRVVEPLGIERLRVSGFGHLPDEVFDDELFP
                     RRRFVVSDDVTRQYTCQSTMPDASLEEIAAAVYPSDCSRSYRYYALDIDEPANVAGVT
                     QAVSEIIGDLNATLPAALREAGLGYLPILTTQADLDARVDHSVQPTVVAFELFGVLAG
                     AATLLVTVLGAARALRQARVTNDSIEPLGMERGARAAAIGAPVAGAVAVGVVVAIAVG
                     YAGSRVGPAGQVRRVDTNGSFSLPAAAWLVPVTAFAVLFAVTFLVGALRVTRRQLQPV
                     SRRAAVGPKLSAAHPAMTDGVRNALSSRRGGALLTGACCVAIAALAGAAVFGANLTRL
                     VDSPPRYGWPWNAAVITNGGYGSANIEQIAHDLDDRDDVGSYDLLGFIGAVMGGDNVT
                     LLVSGRDADPVDLPIVEGRAPREPGEVALGSVTADRLGLGVGDDVPTDIGEGTEPLKI
                     VGVAVMPAIGQIFADRSGLGVGAWTIAPPEQMNEEFVTFVGVDLDSEPAPAFLADIGD
                     ALMSWDARGEPPRTYTQPIRPAEIVNAEEMQRGPLLLAGLLALALLSALALSMAATVN
                     ARRRDYAIFRSVGFTTRQVASSVRWLAITTVAIGVVVGIPAGVLLGRWTWRVFAGDLG
                     AALDVTLPILLLALIAILALIAALLAAAHPAYVARRFRPAETLHTG"
     gene            complement(2889..3233)
                     /locus_tag="VFX21_08305"
     CDS             complement(2889..3233)
                     /locus_tag="VFX21_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6614 family protein"
                     /protein_id="HEX5096000.1"
                     /translation="MDVYEISVDLRPGVRDTEFVAALDAYLGMLQRDGRIETWRLLRR
                     KLGLGSGTEFKVLIETTDLAQLDAAFTEVSTRAEPVEGAHHGVNSLVTNFQAALYRDF
                     PDPHRKTGNERF"
     gene            3352..3846
                     /locus_tag="VFX21_08310"
     CDS             3352..3846
                     /locus_tag="VFX21_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HEX5096001.1"
                     /translation="MPGNGRAAWGWHRLTDDWAHEIVAAANVRRGELVLDIGAGDGAL
                     TRPLLDTGARVIAIEIHPGRLQTLRRRFADEPRCTVVRADAADLYLPRRPFRVVANPP
                     FHVSSALLRRLTARGSRLVAADLVLQRAVAQKWAAGGSLRPLPRAAFSPRPRVDAAVL
                     ILRR"
     gene            complement(3830..>4387)
                     /locus_tag="VFX21_08315"
     CDS             complement(3830..>4387)
                     /locus_tag="VFX21_08315"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5096002.1"
                     /translation="LHLLVNNAGLGDLRPLHTIDDRVWHRVLDVNLTGTFNTMRAAVP
                     LMQRAGQGAIVNNASVSGFTPTRNEAVYSAAKAAVIALTKSGALEYGPAIRVNCVAPG
                     YVRTPLTAAFERHPQAFAPIADGMPMRRMGEASEIAEVIAFLCSRKASYVTGQTVVVD
                     GGVSLPQNGTDAALAKMFSLLNDGE"
BASE COUNT          609 a         1509 c         1543 g          726 t
ORIGIN      
        1 tcgccggcaa cgtcgtcgac ggcagcctcc tcgtcacgct cggcgagttg tcggcagcga
       61 agcgggccgc aatcgtcgtc gaggatcgtt gggcggatgt cttccgcctg cagcacgtcg
      121 caccttcgat ggtgagcgag atgctggcgg ccgcgcaggt gcgctacccg aacgtgccga
      181 tcgtgttctg cgagaaccgc aagctggccc aagaatggac ctaccggttc ctcggagccg
      241 cgcgggcgta cgccgacgag gagaccttcg agttgtaggc cgcaggggtg ctggcacccg
      301 tgcccgagtc gcacgggtac gggggacaac cgcaccgcgg tgtgcatgaa ttgagtcgtg
      361 ctcgccgcgg tgcggatggt ggggcggtcg gaacgacgcc ggcagcggtg ggcgttgctc
      421 gcgctggcgg ccgtcgtcgc gctcggcctc ggcgcgtcgc tcgcgtcgtt cgcggccgcg
      481 taccgcaccg accatgcgtg ggatcagtac ctccggcgcg ccgcggtcac cgacctcgtc
      541 gtgaacccgt cactcccgac gcaggagttc gacgatcgat tgcgcgaagt gccacatgtg
      601 cgcggcatgt ggacgcagga cctgctcgcc gcgggtgtct tcaatacgac tggtccgatc
      661 acggccgcgg aagcggccga ctccgacgca cccggtgaag gcatcggctc ggtcgacggt
      721 cgttacacga aggccgatcg gctcatggtc gacagcggac gcgcgccgag cggcactcac
      781 gaagtcttcg tgacggagac tttccgcccg cggctcgacg agatcatggg tcatcgggtg
      841 caggtcggcg acgagattcc ggtctcgttc ttcgccgcga gcgacgtcag ccccgatgtg
      901 tcgtcggact tcgaccccaa ccgggtcgtc gaaccgctgg gtatcgagcg gttgcgcgtg
      961 agcgggttcg gtcacttgcc cgacgaggtg ttcgacgacg aactgttccc gcgccgccgg
     1021 ttcgtcgtga gcgacgacgt cacccggcag tacacctgcc agagcacgat gcctgatgcc
     1081 tcgctcgagg agatcgccgc ggccgtgtat ccctccgact gctcgcgcag ctaccgctac
     1141 tacgccctcg atatcgacga gcccgccaat gtcgcggggg tcacccaggc cgtcagcgag
     1201 atcatcggcg acttgaacgc gacgcttccc gccgcgctgc gggaagcggg gctcggatac
     1261 ctgccaatct tgacgacgca agcggatctc gacgcgcgcg ttgatcactc ggtgcaacca
     1321 acggtggtcg cgttcgaact cttcggagtc ctggccggcg cggcaacgct gctcgtcact
     1381 gtcctcggtg cggcgcgcgc cctacggcag gcgcgtgtga ccaacgattc gatcgagccc
     1441 ctcgggatgg agcgcggcgc ccgggccgcg gccattggtg cgccggtggc cggtgcagtc
     1501 gcggtcggag tcgtcgttgc gatcgccgtg ggctacgccg gttcgcgtgt cgggccggcc
     1561 gggcaggtgc ggcgtgtgga cacgaacggc tccttctcgt tgccggccgc ggcgtggttg
     1621 gtgccggtca cggcgttcgc cgtcctgttc gccgtgacat tcctcgtcgg cgcgctgcgg
     1681 gtcacgcgcc ggcaactgca gcccgtgtcg cgccgcgcag cagtgggacc gaagctctcc
     1741 gccgcgcacc ccgcgatgac cgacggtgtg cgcaatgcac tctcgtcccg gcggggcggg
     1801 gcactgctca cgggcgcgtg ctgcgttgcg atcgcggcgc tggccggtgc ggccgtgttc
     1861 ggcgcgaacc tgacgagact cgtcgacagc ccgccgcggt acgggtggcc gtggaacgcg
     1921 gcggtgatca ccaacggcgg ctacggcagt gcgaacatcg aacagatcgc gcatgatctc
     1981 gacgaccgcg acgacgtcgg ctcgtacgat ctgctcggct tcatcggtgc ggtcatgggc
     2041 ggcgacaacg tcacgctcct ggtttcggga cgcgacgcgg atcccgttga cctgccgatc
     2101 gtcgaagggc gggcgccgcg cgagcccggt gaggtcgcgc tcggatcggt caccgcggat
     2161 cggctcgggc tcggcgtcgg cgacgacgtg ccaaccgaca tcggtgaagg gaccgaaccg
     2221 ctgaagatcg tcggcgtcgc ggtcatgccg gcgattgggc agatcttcgc cgatcgatcc
     2281 gggctcggcg tcggtgcgtg gaccatcgcg ccacccgagc agatgaacga agagttcgtc
     2341 acgttcgtcg gcgtggactt ggactccgaa cccgcgccgg cgttcctcgc cgacatcggt
     2401 gacgcactca tgtcatggga cgccaggggc gaaccaccgc gtacgtacac acagccgatc
     2461 cgaccggccg agatcgtgaa cgcggaagag atgcagcgcg gcccgctcct gctcgccggt
     2521 ctgctcgcac tggcgctcct gagcgcgctc gcactgtcga tggcagccac cgtcaacgcg
     2581 cgtcgacgcg actacgcgat cttccgctcc gtcgggttca cgacccggca ggtggcgagc
     2641 agcgtgcggt ggctggcgat caccacggtc gccatcggtg tcgtcgtcgg catcccggcg
     2701 ggtgtcctgc tcggccggtg gacgtggcgc gtcttcgccg gggacctcgg cgccgcgctc
     2761 gacgtcacgc tgccaatctt gttgctcgcg ctcattgcga tcctcgcact gatcgccgcg
     2821 ctcctggcgg ccgcacaccc ggcgtacgtc gcgcgccggt tccgtccggc cgagaccttg
     2881 cacacgggct agaacctctc gttgccggtc ttgcggtggg ggtcggggaa gtcgcggtac
     2941 agcgccgctt ggaagttcgt cacgagcgag ttcacgccgt ggtgcgcgcc ctcgaccggt
     3001 tcggcgcgcg tcgagacctc ggtgaacgcg gcatcgagct gcgcgaggtc ggtcgtctcg
     3061 atcagcacct tgaactcggt gccgcttccc aacccgagct tgcgccgcaa cagccgccac
     3121 gtctcgatcc ggccgtcacg ctgcagcatc ccgaggtagg catcgagggc ggcgacgaac
     3181 tcggtgtcgc gcacgcccgg gcgcaggtcg acggagatct cgtagacgtc caagccgcca
     3241 gtctcccacg actccaggcg cctcgaagcc ggagtaattc cggcggagcg acgcctcgac
     3301 gcgcgtacgg tgggtcgcgt atgggttgct tcatcatgac ggttcgtcgc cgtgcccggg
     3361 aacgggcgtg cggcgtgggg ctggcaccga ctcactgacg actgggcgca cgagatcgtc
     3421 gccgccgcga acgtgcggcg cggtgagctc gtgctcgaca tcggcgccgg cgacggcgcg
     3481 ctcacccgcc ccctcctcga cacgggcgcg cgcgtcatcg cgatcgagat ccatccgggg
     3541 cgtctgcaga cgttgcgacg ccggttcgcg gatgaaccgc gctgcacggt cgtgcgggcc
     3601 gacgcggccg acttgtacct gccgcgccgg ccgttccgcg tcgtcgccaa cccgccgttc
     3661 cacgtgtcga gcgcgctgtt gcgccggctg accgcgcgcg ggtctcgttt ggtcgcggcc
     3721 gacctcgtcc tccaacgcgc agtggcgcag aagtgggcgg ccggtggatc gctgcggccg
     3781 ctcccgcgcg ccgcgttctc gccgcgccct cgagtggacg cggcggtcct cattctccgt
     3841 cgttgagcag cgagaacatc ttcgccagcg cggcgtcggt gccgttctgg gggagcgaga
     3901 cgccgccgtc gacgacgacg gtctggccgg tcacgtagct cgccttccgc gagcacaaga
     3961 acgcgatcac ttccgcgatc tccgacgctt cccccatccg acgcatcggc atcccgtcgg
     4021 cgatcggcgc gaacgcctgc ggatgccgtt cgaacgcggc cgtcaacggt gtgcggacat
     4081 aacccggtgc gacgcagttg acgcgaatgg ccggcccgta ctcgagcgca ccgctcttgg
     4141 tgagcgcgat caccgcggcc ttggccgccg agtacaccgc ttcgttgcgg gtcggggtga
     4201 agccggagac cgacgcgttg ttcacgattg cgccctgtcc ggcccgctgc atgagcggca
     4261 ccgccgcgcg catcgtgttg aacgtgccgg tcaggttcac gtcgagcacg cggtgccaga
     4321 ccctatcgtc gatcgtgtgc aagggccgca agtcgccgag ccctgcgttg ttcacgagga
     4381 ggtgcag
//
LOCUS       DASUGA010000420         2683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1785391, whole genome shotgun sequence.
ACCESSION   DASUGA010000420 DASUGA010000000
VERSION     DASUGA010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1785391"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="VFX21_08320"
     CDS             complement(<1..1032)
                     /locus_tag="VFX21_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018889611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5096003.1"
                     /translation="MSTPIPKIISVDDHVVEPPHLWDRWLPAKYRDRGPKSERRGIGS
                     MRHIGGGAYEQTFDDDGPKADCWVYEDLVYINKRHVAAVGFDRDDMTMSPITYDEMRP
                     GCYDPKARVEDMEMNWVEASLSFPTFPRFCGQTFLEAKDRDLAHACVVAYNDWMVDEW
                     CRDSGGRLIPLIIIPLWDAGLAAAEVRRNAARGVHAVCFSEIPPKLGLPSIHSGDWDP
                     FFTACEETDTVVCMHIGSSSQMPATSPDAPVAVAATLSFNNAMASLSDFLFSGVLVRF
                     PRLKLAYSEGQIGWLPYVLERADDVWLEHRAWAGVRNIVPEPPSTYYYRNVYGCFFRD
                     KHGLDSLARV"
     gene            1185..2321
                     /locus_tag="VFX21_08325"
     CDS             1185..2321
                     /locus_tag="VFX21_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5096004.1"
                     /translation="MDLRYSDADEKFRTDLRAWLDATLPSIPPEPSRDAWAERRKWDT
                     DWQRRLYDAGYAGLHWPAEFGGRGASPTEQLIFYEENARARAPYVGVNFVGTLHAGPT
                     LIEEGTDEQKAAHLPKILRGEEVWCQGFSEPGAGSDLASLRTRADRDGDDYVLNGQKI
                     WCSFGQVADIGEFLVRTDPNAPKHKGISWVMLPMDLPGIEVRPLKTVLGSSEFAEVFL
                     TDVRVPVANRVGAENDGWRVTNVTLKYERGTAFVGELVDSIRLCADLAPLVTDPAQWR
                     ELGRCAADFDALWALTKRSVSQSARGVAGAPAMVTKLAYSEARQRFGELCLRVLERDA
                     LHLDDNELVEERLRTLALTIAAGASQIQRNIIGERLLGLPREPR"
     gene            2321..>2683
                     /locus_tag="VFX21_08330"
     CDS             2321..>2683
                     /locus_tag="VFX21_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX5096005.1"
                     /translation="MDLELNDDQVALRDGIVAMLSGRIDGERVRAGFDRALFDELGGA
                     GVFSLRADGFTWADTVVVFEQLGRFCVPGPLVGSLLAGEGTITAITNRSRPAWIEHAN
                     TLDALLVLDDDGLWRVAPD"
BASE COUNT          435 a          888 c          947 g          413 t
ORIGIN      
        1 gacgcgcgcg agcgagtcga ggccgtgctt gtcgcggaag aagcagccgt acacattgcg
       61 gtagtagtac gtcgagggcg gctcggggac gatgttgcgc acgccggccc aggcgcggtg
      121 ctcgagccac acgtcgtcgg cgcgctccaa cacgtagggg agccacccga tctggccttc
      181 ggagtatgcg agcttcaagc gcgggaagcg cacgagcacg ccggagaaca agaagtcgga
      241 gagcgaggcc atcgcgttgt tgaacgacag cgtggccgcg accgcgactg gcgcgtccgg
      301 tgaggtagcc ggcatctgcg acgacgaacc gatgtgcatg cacacaacgg tgtcggtctc
      361 ctcgcacgcg gtgaagaacg ggtcccagtc gccggagtgg atcgacggca agccgagctt
      421 gggcgggatc tcgctgaagc aaaccgcgtg cacgccccgt gccgcgttgc ggcgcacctc
      481 ggccgcggcg aggcccgcgt cccacaacgg aatgatgatc agcgggatga ggcggccgcc
      541 gctgtcgcgg caccactcgt cgaccatcca gtcgttgtac gcgacgacac aggcgtgcgc
      601 gagatcgcgg tccttcgcct cgagaaacgt ctgcccgcaa aagcgcggga acgtcgggaa
      661 cgagagcgat gcctcgaccc agttcatctc catgtcctcg acgcgcgcct tggggtcgta
      721 gcaaccgggg cgcatctcgt cgtaggtgat gggcgacatc gtcatgtcgt cgcggtcgaa
      781 gccgacggcg gcgacgtggc gcttgttgat gtagacgagg tcttcgtaga cccagcagtc
      841 ggccttgggt ccgtcgtcgt cgaaggtctg ctcgtacgcg ccgccgccga tgtggcgcat
      901 ggagccgatg ccgcgccgct ccgacttggg tccgcggtcg cggtacttcg ccggcagcca
      961 ccggtcccac aggtgcggcg gctcgaccac atggtcgtcg acactgatga tcttcgggat
     1021 gggcgtactc acgcgcgcga ccctagactc cggtggctcc aacggccgat ctgctccgac
     1081 gtccaacaag gcggcgccgg agcttgcgga ggcgcttgcg gagcggagcg cgggccaacg
     1141 gtccgggctg cctcacgcga gcacgtgcgg agcgacaaag aaacatggat ctgcgttact
     1201 cggacgccga cgagaagttc cgcaccgact tgcgcgcgtg gctcgacgcg acgttgccga
     1261 gcatcccgcc ggagccgtcg cgcgacgcgt gggccgaacg tcgcaagtgg gacaccgatt
     1321 ggcagcgccg cctctacgac gcgggctacg cgggcttgca ctggccggcc gagttcgggg
     1381 ggcggggcgc gtcgccgacg gagcaactca tcttctacga agagaacgcg cgcgcccgcg
     1441 cgccgtacgt cggcgtgaac ttcgtcggca cgttgcacgc gggaccgacg ctgatcgaag
     1501 aaggcaccga cgagcagaag gccgcgcacc taccgaagat cctgcgcggc gaggaagtct
     1561 ggtgtcaggg attctccgag cccggcgccg gctccgacct cgcgtcgctg cgcacccgcg
     1621 ccgatcgcga cggtgacgac tacgtgctca acgggcagaa gatctggtgc tcgttcggtc
     1681 aggtcgccga catcggcgaa ttcctggtcc gcaccgaccc gaacgcgccg aagcacaagg
     1741 gcatctcgtg ggtcatgttg ccgatggacc tccccggcat cgaggtgcgg ccgctcaaga
     1801 cggtgctcgg ctcgtcggag ttcgccgaag tcttcctcac cgacgtgcgg gtcccggtcg
     1861 cgaaccgcgt cggcgcggag aacgacggct ggcgcgtcac caacgtcacc ctgaagtacg
     1921 agcgcggcac cgcgttcgtc ggtgaactgg tcgactccat ccgattgtgc gccgatctcg
     1981 cgccactcgt caccgatcct gcgcagtggc gcgagctggg ccgctgcgcc gccgacttcg
     2041 acgcgctctg ggcgctcacc aagcgcagcg tctcgcagtc ggcacgcggc gtcgccggcg
     2101 cgcccgcgat ggtcaccaag ctcgcgtact cggaggcgcg ccaacgtttc ggcgagctgt
     2161 gcctgcgcgt gctggaacgc gatgccttgc acctcgacga caacgagctc gtcgaggagc
     2221 ggctccgtac gctcgcattg acgatcgcgg ccggtgcgtc gcagatccaa cgcaacatca
     2281 tcggcgagcg cctgctcggc ctgccccggg agccgcggta gtggacctcg agctgaacga
     2341 cgaccaggtc gcgttacgag acggcatcgt cgcgatgttg tcggggcgca tcgacggcga
     2401 acgtgtgcgc gccgggttcg accgtgcgct gttcgacgag ctcggaggcg cgggcgtctt
     2461 ctcgctgcgc gcggacgggt tcacctgggc cgacaccgtt gtggtgttcg aacagctggg
     2521 ccggttctgc gtgccgggcc cgctcgtcgg atcgttgctc gcgggcgagg gcacgatcac
     2581 tgcgatcacg aaccgatcgc gaccggcgtg gatcgagcac gcgaacacgc tcgacgcgtt
     2641 gctcgtgctc gacgacgatg gcttgtggcg agtggcaccc gac
//
LOCUS       DASUGA010000421         2826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1501440, whole genome shotgun sequence.
ACCESSION   DASUGA010000421 DASUGA010000000
VERSION     DASUGA010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501440"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..1031
                     /locus_tag="VFX21_08335"
     CDS             48..1031
                     /locus_tag="VFX21_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5096006.1"
                     /translation="MTELEPRWFLAGGAGFIGSHFVDRLLGAPEVRRVTLFDNFSSGQ
                     EWHFAHHRGDDRLAVVRGDARDVDALTDAMAEHDVVLHLASNPDIAAAIANPDIDFEQ
                     GTLLTRGVVEAMRRSGVRRILYASGSGIYGDLGEFAPAEDHGPLEPVSTYGASKIAGE
                     TLIAAYAHMFGIRGCAFRFGNVVGPRQTHGVGFDFVRKLLQDPTELEILGDGRQSKSY
                     IHVADVIDAVLLAHTRVASPFDAFNVATGDYITVTEIAELAVAAAGLNAAAVRFRYTG
                     GRSGWLGDVPVMRLDTTKIRDLGWVCRRNTRDALRDALDALVVDAARPTRT"
     gene            1028..1579
                     /locus_tag="VFX21_08340"
     CDS             1028..1579
                     /locus_tag="VFX21_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEX5096007.1"
                     /translation="MTNGLTSRAATFLDRDGVLNVAPVVDGVPRPASPGDVQLLDGVE
                     DACAALVRAGLVLVVVTNQPDVARGTTTRAEVDEIHHWLAARLPITEFRVCTHDDGDG
                     CDCRKPAPGLLQHAAADLDLDLARSVMVGDRWRDVEAGRAAGVRTVFVDHGYAEALPH
                     PADVTVPDLATAVPFIIEHARGG"
     gene            1616..2329
                     /locus_tag="VFX21_08345"
     CDS             1616..2329
                     /locus_tag="VFX21_08345"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005771862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX5096008.1"
                     /translation="MPVDLGTLKVFADGADRAGILRYRAHPLIQGFTTNPTLMRKAGV
                     DDYESFALELVELVPDLPISFEVFADDFDDMERQALKIAAWGTNVYVKIPVTDTSGRS
                     TVPLQRRLADQGVQTNVTALMTVPQVEAVADAIGSGPSAFISVFAGRIADTGRDPVPL
                     LQDALAVIADRPHLEMIWSSPREVLNIVQAAAIGCHVITVTYDLLEKLPIFDKDLDEY
                     SLETVRMFFDDAARAGYSL"
     gene            2495..>2826
                     /locus_tag="VFX21_08350"
     CDS             2495..>2826
                     /locus_tag="VFX21_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096009.1"
                     /translation="MKKLITLIVAALVGTSSFATIWASGVAAQADATAVTPASASTDP
                     GIFVGWGRPAFGAIGSPPTGACKSTDVCEHPSNGISTFGVTAVSAGHVHTLLLRADGT
                     VWAVGDNR"
BASE COUNT          419 a         1029 c          932 g          446 t
ORIGIN      
        1 gagccacccg tcgctgcagc agtccgccac caagtgggaa tcgctcgatg actgagctcg
       61 agccgcggtg gttcctcgcg ggcggcgcgg gattcatcgg cagccacttc gtcgaccgac
      121 tcctcggcgc acccgaagtc cgccgcgtca cgctcttcga caacttctcg tcggggcagg
      181 aatggcattt cgcgcatcat cgcggcgatg accggctcgc cgtcgttcga ggggacgcgc
      241 gcgacgtcga cgccctcacc gacgccatgg ccgagcacga cgtggtgctc cacctcgcgt
      301 ccaaccccga catcgcggct gcgatcgcga accccgacat cgacttcgag cagggcacgc
      361 tcctgacccg cggcgtggtc gaggccatgc gccgatcggg cgtgcgccgc atcttgtacg
      421 cctcgggcag cggcatctac ggcgacctcg gcgagttcgc gccggccgag gaccacgggc
      481 ccctcgaacc cgtctccacc tacggcgcga gcaagatcgc gggcgagacc ctgatcgcgg
      541 cgtacgccca catgttcggc atccgcggct gcgccttccg cttcggcaac gtggtgggac
      601 cgcgccagac gcacggggtc gggttcgact tcgtccggaa gctgctccag gatcccacgg
      661 agctggagat cctcggcgac ggtcgccaga gcaagtcgta catccacgtt gccgacgtca
      721 tcgacgccgt gctcctggcg cacacgcgtg tcgcgtcccc gttcgacgcc ttcaatgtcg
      781 cgaccggtga ctacatcacc gtcaccgaga tcgccgagct ggccgtcgcc gcggccggtc
      841 tgaacgcggc tgccgtccgg ttccgctaca ccggcggccg cagcgggtgg ctcggcgacg
      901 tcccggtgat gcgactcgac accacgaaga tccgtgacct cggctgggtg tgccgacgaa
      961 acactcgcga cgcgctccgc gacgcgctcg acgcgctcgt cgtcgatgcc gcgaggccga
     1021 cgcgcacgtg accaacggtc tcactagccg tgccgcgacc ttcctcgatc gcgatggcgt
     1081 gctcaacgtc gccccggtcg tcgatggggt gcctcgtcca gcgtcgcccg gagacgtgca
     1141 gctgctcgac ggcgtcgagg atgcgtgcgc ggccctcgtg cgcgccgggc tcgtcctcgt
     1201 ggtggtgacg aaccagcccg acgtcgcgcg cggcacgacg acgcgggccg aggtcgacga
     1261 gatccaccac tggctggccg cacgtctccc gatcaccgaa ttccgggtgt gcacgcacga
     1321 cgacggtgac ggctgcgact gccgcaagcc ggcgccgggg ctgctgcagc acgcagccgc
     1381 cgacctcgat ctcgatctcg cgcggagcgt catggtcggt gatcgctggc gagacgtcga
     1441 ggccggccgc gccgccggcg tccgcaccgt gttcgtggac cacggatacg ccgaggcgct
     1501 cccgcaccca gccgacgtca cggtgcccga tctcgccacc gcggtgccct tcatcatcga
     1561 gcacgcacgc ggtggttgac cgatccggcg acccgcccga ataggctcct tcgtcatgcc
     1621 ggtcgatctc ggcacgctca aggtgttcgc cgacggcgcc gaccgcgccg gcatcctgcg
     1681 ctaccgcgcg catccgctca tccagggctt caccacgaac cccacgctga tgcgcaaggc
     1741 cggcgtcgac gattacgagt cgttcgcgct cgagctggtc gagctcgtcc ccgacctccc
     1801 gatctcgttc gaggtgttcg ccgacgactt cgacgacatg gagcgccagg cgctgaagat
     1861 cgcggcgtgg ggcaccaacg tctacgtgaa gatccccgtc accgacacga gcggtcgatc
     1921 cacggtgccg ctccaacgcc gtctcgcgga ccagggcgtg cagacgaacg tcaccgcgct
     1981 catgacggtc ccgcaggtgg aggccgtcgc cgacgcgatc gggtcggggc cgagtgcgtt
     2041 catctcggtg ttcgcaggcc ggatcgccga cacgggtcgc gacccggttc cgctcctcca
     2101 ggacgcgctc gccgtgatcg cggatcgacc gcacctcgag atgatctggt cgagtccgcg
     2161 cgaggtgctg aacatcgtgc aggcggccgc gatcggctgc cacgtgatca ccgtgacgta
     2221 cgacctgctc gagaagctgc cgatcttcga caaggacctc gacgagtact cgctcgaaac
     2281 ggtgcggatg ttcttcgacg acgccgcgcg cgccggctac tcgctctgat ccgctccgcg
     2341 cgttcggcga gcgacggcac cacccgcacg cggctccctc tccccggggt gcgtgtcaga
     2401 cacttcttgc acttttgcca tgttgcccat cccggtgagt ccgcacactc ggccgcatga
     2461 cggagaacgg gcgggcgacg aaggggctca caccgtgaag aagctcatca ccttgatcgt
     2521 tgccgcgctc gtgggcacgt cgtcattcgc gacgatctgg gcgtcaggtg tggcagcgca
     2581 ggccgacgcg actgcagtca cgcccgcctc ggcgtccacc gacccgggga tcttcgtggg
     2641 ttggggccgc cccgcgttcg gcgcgatcgg atcgccgccg accggagcct gcaagtcgac
     2701 ggacgtctgc gagcacccgt cgaacgggat ctcgaccttc ggcgtcacgg ccgtgtccgc
     2761 agggcacgtg cacacgttgc tccttcgggc tgatggcacg gtgtgggccg tgggcgacaa
     2821 tcgcta
//
LOCUS       DASUGA010000422         2625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1953321, whole genome shotgun sequence.
ACCESSION   DASUGA010000422 DASUGA010000000
VERSION     DASUGA010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1953321"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VFX21_08355"
     CDS             <1..617
                     /locus_tag="VFX21_08355"
                     /inference="COORDINATES: protein motif:HMM:NF022059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX5096010.1"
                     /translation="GEVELGTPLRTVIDTVGSGLATGRAVKAVLSGVANPVVTAQDLD
                     VPTSYEGFQSIGSGMGAAGFIVYDDTACMVNVACLVSRFLYVESCGQCPPCKIGSGEI
                     TVHLEQLEAGTGSDEDLEGIIHWLERVTDGHRCYLAVEEQVTVGSLLRAFADEFGAHV
                     EHRKCPRPRHLSVPKLVDLAGGTATYDETFARKRPDWTYDLTGP"
     gene            681..1475
                     /locus_tag="VFX21_08360"
     CDS             681..1475
                     /locus_tag="VFX21_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5096011.1"
                     /translation="MQLILVGDDGSRAASHAVTWATRLADERDARLFIVHVASTSESV
                     ESDDRVERITVRDSHPASAIMETAEDRDVDLIVLGRRGRGGFPSLPIGTTAHHVAAAS
                     GRPVAVVPPIETTTSGPVVQRAVVGIDGFPGSEEAAAWSARQWPHAHFNAVQAVELAP
                     AFAQLDDGTGAEIYERAHARAVTLLRDRWSRPLVEAGVAFDLAVEDGGPAEVLLEVAT
                     KVGADVIVVGRRDHAPLRGTLGGVSQRVLAYAPCAAVIVPPPSSPG"
     gene            complement(1520..1942)
                     /locus_tag="VFX21_08365"
     CDS             complement(1520..1942)
                     /locus_tag="VFX21_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5096012.1"
                     /translation="MPGPAFTDEGANPFVVDIEAATLGNENYRSTLWTGAHLQMTVMH
                     IEPGHDIGLEVHPDGDQFLRVEAGHARVQMGPQRDELSFEREVEDDWVILVPAGCWHN
                     VTNIGKEPLKVYALYAPPEHPHGTTHRTKAAAEAAEHH"
     gene            complement(1983..>2625)
                     /locus_tag="VFX21_08370"
     CDS             complement(1983..>2625)
                     /locus_tag="VFX21_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5096013.1"
                     /translation="ESVLELDDGTEVRGDVVVFGTGRTPRTRDLGLEQAGAVIDDQGA
                     IVVDEHCRGADGIWAIGDVTGLMPFTHVAKYQGRVVADCILGTTRVARYEGIPRVVFG
                     DPEIAAVGLTAAGAGDRGITTTSATVDLPEKIARPWTYELEPRGELGLLVDTGSDVLV
                     GAWAVAPLAGEWIHQAALAIRARVPIDTLLDQVAQFPSYTEGYLNALEALPRP"
BASE COUNT          408 a          876 c          891 g          450 t
ORIGIN      
        1 tcggcgaagt cgagctcggc acgccactcc gcaccgtcat cgacacagtc ggcagcggtc
       61 tcgcaacggg ccgggccgtc aaagcggtgc tctccggagt cgcaaacccg gtcgtcaccg
      121 cccaggatct cgatgtcccc acgagctacg agggcttcca gtcgatcggc agcggcatgg
      181 gtgcggccgg cttcatcgtc tacgacgaca ccgcgtgcat ggtgaacgtg gcctgtctgg
      241 tgtcacggtt cctctacgtg gagtcgtgcg gccagtgccc accatgcaag atcggttcgg
      301 gcgagatcac cgtacacctc gagcagctcg aagcggggac gggaagcgac gaggacctcg
      361 aggggatcat ccactggttg gagcgcgtca cggatggcca ccggtgttac ctcgcggttg
      421 aagaacaggt cacggtcgga agcttgctgc gcgcgtttgc ggacgagttc ggcgcgcatg
      481 tcgagcatcg caagtgccca cggccacgac acctttcggt cccgaagctc gttgatctgg
      541 ccggcggtac ggcgacctac gacgaaacct tcgcgcgcaa gcgccccgac tggacgtatg
      601 acctcactgg cccctgaacg aacgctccca gcgggtagtg atgcattgac gaattcctca
      661 ctcgggaggt gggacgacct atgcagctca tcctggtcgg tgacgacggt tcgcgcgcag
      721 cttcccatgc cgtcacatgg gcgacgcgtc tcgcagacga gcgcgacgca cggctcttca
      781 tcgtgcacgt cgcctcgaca agtgagtccg tcgagtccga cgaccgcgtc gaaaggatca
      841 ccgtgcgcga cagccatccg gcctcggcga tcatggagac ggccgaggac cgcgacgtcg
      901 acctgatcgt gctcgggcgc cgcggccgcg gcgggttccc gtcgctcccg atcggcacga
      961 ccgctcatca cgtcgccgcc gcgtcggggc gcccggtcgc ggtggtaccg cccatcgaga
     1021 ccacgacgag cggcccggtc gtgcagcgcg ccgtcgtcgg gatcgacgga ttccccgggt
     1081 cggaggaggc cgcggcctgg tccgcgcgcc aatggccgca tgcccacttc aatgcggtgc
     1141 aagcggtcga gctcgcccca gccttcgcgc agctcgatga cggtaccggt gccgagatct
     1201 acgagcgtgc gcacgcgcgc gcagtcacgc tgctccgaga ccgctggagc cgaccgctgg
     1261 tcgaagcagg cgttgcgttc gaccttgctg tcgaggacgg cggtccggcc gaggtgctcc
     1321 tcgaggtcgc gacgaaggtc ggtgccgacg tcatcgtcgt cggtcgacgc gatcacgctc
     1381 cccttcgggg cacgctcggc ggtgtcagcc aacgcgtcct tgcgtatgcg ccgtgcgccg
     1441 ctgtgatagt cccgcctccg tcatcaccgg ggtaagggcc tcgagcgaat tggggtagcc
     1501 ctcggtgtga ctcggcgtct cagtggtgtt cggccgcctc ggcggccgct ttggtgcgat
     1561 gtgtcgtgcc atgcgggtgc tcgggtggcg cgtagagcgc gtagaccttc agcggttcct
     1621 tgccgatatt cgtgacgttg tgccagcagc ccgcgggcac aaggatgacc caatcgtcct
     1681 cgacctcgcg ctcaaaggac agctcgtctc gttgtgggcc catctgcacc cgagcatgtc
     1741 cggcttcgac gcgcaggaac tgatcgccgt cggggtgcac ctccaagccg atgtcgtgac
     1801 ccggttcgat atgcatgact gtcatctgga ggtgcgcgcc ggtccacaac gtgctgcggt
     1861 agttctcgtt gccgagcgtg gcagcttcga tgtcgacgac gaacgggttc gcaccttcgt
     1921 cggtgaacgc agggccgggc atcggcgctc ctctctcagg gttcatgcgg gtgccttcaa
     1981 actcaggggc ggggtaatgc ctcgagcgca ttgaggtagc cctcggtgta gctcgggaac
     2041 tgcgcgacct gatcgagcag cgtgtcgatc gggacgcgcg cgcggatggc gagcgcagcc
     2101 tggtgaatcc actcacctgc gagcggagca acggcccagg cgccgacgag cacatcactc
     2161 ccggtatcga cgagtaagcc cagctcaccg cgcggctcga gctcgtatgt ccaaggtcga
     2221 gcgatcttct cgggcaggtc gacggtggcc gaggtcgtcg tgatgcctcg atcgccggcc
     2281 ccggcggccg tcaggccgac cgcggcgatc tccgggtcgc cgaacacgac tcgggggatc
     2341 ccttcatacc gggcgacgcg cgtcgtaccg aggatgcagt cggcgacgac tcggccctgg
     2401 tacttggcga cgtgagtgaa gggcatgagc ccggtcacgt ccccgattgc ccagatgccg
     2461 tcggcgccgc gacagtgctc gtcgacgacg atcgcgccct ggtcgtcgat caccgcaccg
     2521 gcctgctcga gtccgagatc acgtgttcgt ggggtgcgcc cggtcccgaa gacaaccacg
     2581 tcgccgcgga cctcggtgcc gtcgtcgagc tcgagcacgc tctcg
//
LOCUS       DASUGA010000423         2533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1945907, whole genome shotgun sequence.
ACCESSION   DASUGA010000423 DASUGA010000000
VERSION     DASUGA010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1945907"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFX21_08375"
     CDS             <1..257
                     /locus_tag="VFX21_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006376485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HEX5096014.1"
                     /translation="GDVRVAARLSDVEPFRVDADGDVLHLAVHLPFTERDEVQLGRTD
                     DELVLTVGPHRRTIVLPDSLVRRSVGGARFVDDDLVVEFI"
     gene            257..472
                     /locus_tag="VFX21_08380"
     CDS             257..472
                     /locus_tag="VFX21_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096015.1"
                     /translation="MTGMPPPPADDAIEHLWNAAHEFLRAVRMLVDAADEFVTQQQAE
                     AARPEREARVHRIDIDVEPHDADADAS"
     gene            469..1410
                     /locus_tag="VFX21_08385"
     CDS             469..1410
                     /locus_tag="VFX21_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX5096016.1"
                     /translation="MTAASTIGLDIGGTKVLGVRLDADGTVQAESRHASPHAGLDALI
                     DTSLHVVRAIAAGDARIGVGIAGLVDDAGNVLYSPNLPTVRRAAMRDEMAKATGCAVV
                     VDNDANVALLGEATYGAAAGASHALLVTLGTGIGGGMYLDGALYRGAHNFGAEFGHFT
                     VDSSGPMCACGERGHWEAIASGSALGRIAREFVGRTGGAAIVAEAGGDAAAVSGMHVG
                     RAAAKGDPDGLHVLAVYADNVALGLAALANIFDPETIVIGGGLVELGPLLFGPLHDAF
                     QRHVEGSQYRPPIQIVPAQLGEHAGAIGAAVLARSLS"
     gene            1407..2153
                     /locus_tag="VFX21_08390"
     CDS             1407..2153
                     /locus_tag="VFX21_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5096017.1"
                     /translation="MTLKVAVTLPSFVEDPEIPLAIARAAEDVELDAVFVYDHLWRGD
                     PPNRRPALECFALLGAVAAETERVGVGTLVARATLRPPATLCNAFATVQRISNGRLLA
                     AIGAGDSQSRAENEAFGLDFGTMAERIDALHGAVRATRGHGYPVWVGGIAAPVREIVT
                     IADGWNRWGGTPEEFVAEAKLVREVAPDAVLTWGGLAKPREEGVDALAARLTPFVDAG
                     AKWIIIGPVDSSNPENVPVLGELRARLTTR"
     gene            complement(2164..>2533)
                     /locus_tag="VFX21_08395"
     CDS             complement(2164..>2533)
                     /locus_tag="VFX21_08395"
                     /inference="COORDINATES: protein motif:HMM:NF036523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096018.1"
                     /translation="TLRERLVQRPPRGITPPARVADVLRRATRALEREPQVTRAMLCA
                     MASNDEGALPFKQEVDDNLRAIIADAVDHPSQRVANLDDIVHVLGSVWFAELTYWSNG
                     LTGPGAMADNLARAAELLLP"
BASE COUNT          375 a          838 c          928 g          392 t
ORIGIN      
        1 acggcgacgt gcgcgtcgcg gcccggctct ccgacgtgga gccgttccgc gtcgacgccg
       61 acggtgacgt gctgcacctt gcggttcatt tgccgttcac cgagcgcgac gaggtgcaac
      121 tgggccggac cgacgacgag ctggtcctga ccgttgggcc gcatcggcgc accatcgtgc
      181 ttccggacag cctggtccgg cgctcggtgg gcggtgcccg tttcgtcgac gacgacctcg
      241 tcgtagagtt catctgatga ctgggatgcc accgccgccg gccgatgacg cgatcgagca
      301 cctctggaac gccgcgcacg agttcctgcg ggcagtgcgg atgctggtcg acgcggccga
      361 cgagttcgta acgcagcaac aagccgaggc ggcgcgtccc gagcgcgagg cgcgggtgca
      421 ccgcatcgac atcgacgtcg agccccacga cgccgacgcc gacgcatctt gaccgcggct
      481 tcgacgatcg gcctcgacat cggcggcacc aaggtgcttg gcgtgcgcct tgatgctgac
      541 ggaacggtgc aagccgaatc ccgccacgca tcgccccatg ccgggctgga tgcgctgatc
      601 gatacgagcc tgcatgtcgt gcgcgcgatc gcggccggcg atgcgcgcat cggcgtcggg
      661 atcgcgggcc tcgtcgacga cgcgggcaac gtcttgtact cgccgaacct gccgaccgtc
      721 aggcgggccg ccatgcgcga cgagatggcg aaggccacgg gctgcgcggt tgtcgtcgac
      781 aacgacgcga acgtggcgct gctcggcgaa gcgacctacg gcgccgcggc cggagcctcg
      841 cacgcgttgc tcgtcacgct cgggacgggc atcggcggcg gcatgtatct cgatggcgcg
      901 ctgtatcgcg gtgcgcacaa cttcggcgcc gagttcgggc acttcacggt cgacagttcg
      961 ggacccatgt gcgcatgcgg tgaacgggga cactgggaag cgattgcatc gggttcggcg
     1021 ctcggtcgga tcgcacgcga gttcgttggc cgtaccggcg gggcggcgat cgtggcggaa
     1081 gcgggcggtg acgcagcagc ggtgagcggc atgcacgtcg gccgggcggc ggcgaaaggc
     1141 gatccggatg ggctgcacgt gctggcggtc tacgccgaca acgtggcgct cgggctcgcg
     1201 gccctcgcga acatcttcga cccggaaacg atcgtgatcg gcggcgggct cgtggagctc
     1261 ggtccactgt tgttcgggcc gttgcacgac gcgttccaac gccacgtcga gggcagccaa
     1321 taccggccgc cgatccagat cgtcccggcg cagctcggcg agcacgcggg cgcgatcggt
     1381 gccgccgtgc tcgcgcggag cttgtcgtga cgctcaaggt cgcggtgaca ctgccgtcgt
     1441 tcgtcgaaga tcccgagatt ccactcgcga ttgcgcgggc agcagaagac gtcgagctcg
     1501 acgccgtgtt cgtctacgac cacctgtggc gaggcgatcc gccgaaccgc cggcccgcgc
     1561 tcgagtgctt cgcactgctg ggtgcggtcg ccgccgaaac ggaacgggtc ggtgtcggca
     1621 cgctcgtggc gcgcgcgacg ttgcggccgc cggccacact ctgcaacgca ttcgcgaccg
     1681 tacaacgcat ctcgaacgga cggctgctcg ccgccatcgg cgcgggcgat tcgcagtcgc
     1741 gcgccgagaa cgaagcgttc gggctcgact tcggcaccat ggcggagcgg atcgacgcgc
     1801 tgcacggcgc cgtgcgcgcg acgcgcggcc acggctatcc ggtgtgggtc ggcggcatcg
     1861 ccgcgcccgt gcgcgagatc gtcacgatcg ccgacggctg gaaccggtgg ggcggaacgc
     1921 cggaggagtt tgtcgcggag gcgaagctcg tgcgggaggt cgcgcccgac gcggtgctga
     1981 cgtggggcgg actcgccaaa ccccgggaag aaggcgtcga tgcgttggcg gcgcggctca
     2041 ctccgttcgt cgacgccggc gccaagtgga tcatcatcgg tccggttgat tcgtcgaatc
     2101 ccgagaacgt tccggtgctc ggcgagctgc gcgcccggct gacgacgcgc tgagaaagca
     2161 gcgttaagga agcaggagtt cggcggcgcg ggccaggttg tcggccatcg cgccgggacc
     2221 ggtcagcccg ttgctccagt acgtgagctc cgcgaaccag accgagccga gcacatgaac
     2281 gatgtcgtcg agattggcga cgcgctggct tggatgatcg accgcgtcgg cgatgatcgc
     2341 gcgcaggttg tcgtcgactt cctgtttgaa cggcagcgca ccttcgtcgt tcgaagccat
     2401 cgcgcacagc atcgctctcg tgacttgcgg ctcgcgctcg agggcgcggg tcgctcggcg
     2461 cagcacgtcg gcgacgcgcg ccggcggcgt gatgccgcgc ggtggacgct gcacaagtcg
     2521 ttcccgcaac gtc
//
LOCUS       DASUGA010000424         6452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1291141, whole genome shotgun sequence.
ACCESSION   DASUGA010000424 DASUGA010000000
VERSION     DASUGA010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6452
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291141"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1830)
                     /locus_tag="VFX21_08400"
     CDS             complement(<1..1830)
                     /locus_tag="VFX21_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5096019.1"
                     /translation="MTCSACGAAVPEGARFCPECGTRLVAAPEERRLVTVLMADLVGF
                     TAFSESADPEQVKRLVDHCFERMAVDITAFGGRLDKIVGDEMVALFGTPVAHEDDAER
                     AVRAALRMQETLVSAAAEIGLQVRMRVGVNTGEVLVGAMRTGGESTVMGDVVNTAQRL
                     ETVAEPGEVIVGLATYTATRDAISYEALGLLSVKGRDEPVEAYRAGAALAPPGRRRAR
                     GEAPLVGREAEMGALQHVVRMAAGRRRGQLVLLYGDAGVGKSRLASELAQLAHNDLGA
                     RVLAGDCVPYGDANVFGPVGEAVRHACRLEHATTPAEVRECVTTTVAEAMGLPADSTE
                     TDRLVEGLLYVMEGITRPGVDPSRARDEGMRAGLAFFEALAAQTPVVLTLSDLHWAED
                     PVLEFADRLLARLRNVPLVLVATARRGFEERWSPSVGHQNTLVLNLDPLDAHATAQLV
                     DSLFEGCADAETVTTLLERSGGNPFFVEELVAYVQESAEDGRTDINDATLRELPATLH
                     GLVAARLDALDVAERSLLEDCAVVGGSGSVDAAVALAQRADARRILDRLADRDLLVLD
                     GDDFHFKSELIREVAYGTLTKAERGRRHAALAPILGARGDGAID"
     gene            2000..3661
                     /locus_tag="VFX21_08405"
     CDS             2000..3661
                     /locus_tag="VFX21_08405"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5096020.1"
                     /translation="MLSLVPEPVFVVAVEHTPHFRFLYGNDAFRELVAWPEDEPVAGD
                     LSVAFPDDTVETYEEHFAVAARDGASVSFETDVAGGRTTISVDVAPVRDGSGCTHLVC
                     TAHDVTDRHRMQASLEYHTRHDALTQLPNRVSLVERLGDALGVAQDLGGNVALLFLDI
                     DDFKLVNDTLGHDAGDAVLTMVARRLERVLREGDIVARFGGDEFGVICNDVECVDEAI
                     AVADRIRHVFEQPMTLDTGQVYLTAGVGIVVSNGGEDQPERMLRDADVARFAAKAEGR
                     GSVSVFDEHMRELMLTRLEVESALRRAIERDEFRVHYQPLVDFEQSEVTGFEALVRWE
                     HPERGLLGPDAFLEAAERTGLIVPIGAWVFDEACRQAAAWAEESAKPLSVSVNVSERQ
                     LLAANFVDSIARALEASGLPPELLVIEVTERVLMDDRERAAEILGRLAELGVQLSVDD
                     FGTGQSLSYLKHLPVHSLKIDRSFIDGLGADLDDAAIVAAIVALGHALGLTVTAEGIE
                     TPRQLSEVRSLGCDTGQGYYFARPQPGEVVRALVHRQIRWRERAS"
     gene            complement(3709..4026)
                     /locus_tag="VFX21_08410"
     CDS             complement(3709..4026)
                     /locus_tag="VFX21_08410"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEX5096021.1"
                     /translation="MDLPEEPPPESVDLDRRVGLTIRWADGTEDRFDLAELRLNCPCA
                     ECRGRREQHEPIWPRAGKPDVLEATGAELVGGWGISLRWNDSHDTGIYAWAMLKAWRG
                     TTS"
     gene            4078..4371
                     /locus_tag="VFX21_08415"
     CDS             4078..4371
                     /locus_tag="VFX21_08415"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015889728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX5096022.1"
                     /translation="MARPSRLSDDDVSAALASLPEWAREGDEIVKTFACASFPDAIAF
                     VVRIGFFAEKADHHPDLDIRWRNVRVALTTHDAGGLTQLDVDLATAIDGAFAG"
     gene            4375..4773
                     /locus_tag="VFX21_08420"
     CDS             4375..4773
                     /locus_tag="VFX21_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096023.1"
                     /translation="MPLVIAIVLVFVLLGILWTAVAREKGPAPQDVALAYENAWDQLD
                     FDLLYDLSGDELRDGLGRKQFIATKKAAYTADRHGRLGAEVAVEESISTGQTALVVTR
                     VTTADGSVRNNVMLEHRPSGWLVVKYSIRA"
     gene            4785..5318
                     /locus_tag="VFX21_08425"
     CDS             4785..5318
                     /locus_tag="VFX21_08425"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX5096024.1"
                     /translation="MVLTGGRSTRMGRDKATIVVDGETWAVRSGRLLAQVCAPVIEVG
                     PGVSGLPAIVEATPGRGPLVAFLAGVDALAATGPVVLLACDMPRLDVALLELVANWPG
                     TGTVVPVVDGHKQHSCSRWSSAAIGRGRNAPDGAIGAMRGDDTVFLEESVWRRVTSAP
                     GLTDADTPEDLARLGLT"
     gene            5315..6157
                     /gene="fdhD"
                     /locus_tag="VFX21_08430"
     CDS             5315..6157
                     /gene="fdhD"
                     /locus_tag="VFX21_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX5096025.1"
                     /translation="MKVSDRAIRKPVSPAQVLHVGADGARRAPDRLVTEEPMEIRAHG
                     PGEEPAPVVVTMRTPGNDFELAAGFLHTEGLLPDAAALDTIAYCLGGEGEQMYNVVTV
                     KARRPIVGRLRERNFLSTASCGICGKTALDDVEVRCAPVGAGPTITADVVRSLPSTLA
                     AQQRVFGETGGLHAAATFASDGSLLTLREDVGRHNALDKLVGHAFLERALPLADQVLL
                     VSGRLSFELVQKAAVAGIPVICAVSAPSNLAVGAAERFGQTVVGFLRDDRFNVYCNPD
                     RVLV"
     gene            6154..>6452
                     /locus_tag="VFX21_08435"
     CDS             6154..>6452
                     /locus_tag="VFX21_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HEX5096026.1"
                     /translation="MSKRDNRIRTSKQLRRDLWVGWKPNGVGEQKPHHYRDMTRTVWE
                     NRKNLPYAWRILRKGVCDGCALGAAGFHDWTITGVHLCTTRLELLKVNTAPAIADG"
BASE COUNT          934 a         2248 c         2276 g          994 t
ORIGIN      
        1 gtcgatcgcg ccgtcgccgc gcgcgcccag gatcggcgcg agcgcggcgt gacgccggcc
       61 gcgctcggcc ttggtgagcg tgccgtacgc gacctcgcgg atgagctcgg acttgaagtg
      121 gaagtcgtcg ccgtcgagca cgagcaggtc gcgatcggcg aggcggtcga ggatgcggcg
      181 cgcgtccgcc cgctgcgcga gcgcgactgc ggcgtcgacc gatccgctgc cgccgacgac
      241 cgcgcagtct tcgagcagtg agcgttccgc gacgtcgagc gcgtcgaggc gcgccgcgac
      301 gagtccgtgc agcgtggccg gcagctcgcg gagggtcgcg tcgttgatgt cggtgcggcc
      361 gtcttcggcc gactcctgca cgtaggcgac cagctcttcg acgaagaaag gattgccgcc
      421 gctacgctcg agcaacgtcg tcaccgtttc cgcgtcggcg cacccctcga acaacgaatc
      481 gacgagctgc gcggtggcgt gcgcgtcgag ggggtcgaga ttgagcacga gcgtgttctg
      541 gtggccgacg gagggcgacc accgttcctc gaacccgcgg cgggcggtcg cgacgagcac
      601 caggggcacg ttgcgcaggc gcgcgagcag ccggtcggcg aactcgagca ccggatcttc
      661 ggcccaatgc aggtcggaga gcgtcaggac caccggcgtc tgcgcggcga gggcctcgaa
      721 gaacgccagg ccggcgcgca tgccttcgtc acgcgcgcgc gacgggtcga cacccgggcg
      781 cgtgatcccc tccatgacgt agagcaggcc ctcgaccaga cggtcggtct cggtcgagtc
      841 ggccgggaga cccatcgcct ccgcgaccgt cgtggtgacg cactcgcgga cctcggccgg
      901 tgtcgtcgcg tgctcgagcc ggcacgcgtg ccgcacggct tcgccgacgg gcccgaacac
      961 gttcgcgtcg ccgtagggca cacaatcgcc cgccagcacc cgggcgccga gatcgttgtg
     1021 ggcgagctgc gcgagctcgc tcgcgagccg gctcttgccg acgccggcgt cgccgtagag
     1081 aaggacgagt tggccgcgcc ggcggccggc ggccatgcgc acgacgtgct gcagcgcacc
     1141 catctcggct tcgcggccga cgagcggtgc ctcgccgcgg gcgcggcgac gacccggggg
     1201 cgcgagcgcg gcgccggcgc ggtacgcctc gaccggctcg tcgcggccct tcaccgacaa
     1261 caggccgagc gcctcgtagg agatggcgtc gcgggtcgcc gtgtacgtcg cgaggccgac
     1321 gatcacctcg cccggttcgg cgacggtctc gaggcgctgc gcggtgttga cgacgtcgcc
     1381 catcaccgtc gactcgccac ccgtgcgcat ggcaccgacg agcacctcgc cggtgttcac
     1441 gccgacgcgc atgcggacct ggagtccgat ctcggcggcg gcgctcacga gtgtttcctg
     1501 catccgcaac gcggcgcgca ctgcccgttc ggcgtcgtcc tcgtgcgcga ccggcgtgcc
     1561 gaagagcgcg accatctcgt cgccgacgat cttgtcgagg cgcccgccga acgcggtgat
     1621 gtcgaccgcc atccgttcga agcagtggtc gacgagccgt ttgacctgct cagggtcggc
     1681 cgattcggag aacgcggtga atccgacgag gtccgccatc agcaccgtga ccaggcggcg
     1741 ctcctcgggc gcggcgacga ggcgtgtccc gcattcgggg caaaaccgcg cgccttcagg
     1801 tactgcggcc ccgcaggccg agcacgtcat gggtcgaggt taccggcctg ctcagacagg
     1861 ccgaccttcc ttccgcggct ccggccgttc aggtctgcgc cgccggtgcc gaaaggactg
     1921 tggcgacggg aaagggacga tggcagttac acagcccgcg gacggagacg cgacggagct
     1981 cgacgaacga gcgctccggg tgctctctct cgttcccgag cccgtgttcg tggttgccgt
     2041 cgagcacaca cctcacttcc gcttcctcta cggcaacgac gccttccggg agctggtcgc
     2101 ctggccggag gacgagcccg tggccggcga cctttccgtc gccttccccg acgacaccgt
     2161 cgaaacgtac gaagagcact ttgcggtagc cgcccgtgac ggcgcatccg tgtcgttcga
     2221 gaccgacgtg gcgggtggcc gtaccacgat ctcggtcgac gtcgcaccgg tgcgcgacgg
     2281 atccggttgc acgcatctcg tgtgcaccgc gcacgacgtc accgaccgcc accgcatgca
     2341 agcgtcgctc gagtaccaca cgcgccacga cgcgctcacg cagctgccga accgcgtgtc
     2401 gctcgtcgag cggttgggcg acgcgctcgg cgttgcgcag gacctcggcg ggaacgtcgc
     2461 gctgttgttc ctcgacatcg acgacttcaa gctcgtgaac gacacgctcg gtcacgacgc
     2521 cggcgacgcc gtgctcacga tggtcgcgcg tcgtttggaa cgcgtgctgc gcgagggcga
     2581 catcgtcgcg cgcttcggcg gtgacgagtt cggcgtgatc tgcaacgacg tcgagtgcgt
     2641 cgacgaggcg attgcggtcg cggaccgcat ccgccacgtg ttcgaacagc cgatgacgct
     2701 cgacaccggc caggtgtatc tcacggccgg cgtcggcatc gtggtctcca atggcggtga
     2761 ggaccaaccc gagcggatgc tgcgcgacgc ggacgtcgcg cgcttcgccg ccaaggccga
     2821 aggccgcggc tcggtctcgg tgttcgacga gcacatgcgc gagctgatgc tcacgcggct
     2881 cgaagtcgag agcgcgctgc gccgggccat cgaacgcgac gaattccgcg ttcactacca
     2941 gccgctcgtg gacttcgagc aatccgaggt caccggcttc gaagcactgg tgcgttggga
     3001 gcaccccgag cgcggcctgc tcggccccga tgcgttcctc gaagccgcgg agcgcaccgg
     3061 cctgatcgtg ccgatcggcg cgtgggtgtt cgacgaagcg tgccggcagg cggcggcgtg
     3121 ggccgaggag tcggcgaagc cgttgagcgt ctccgtgaac gtgtccgagc gccagctgct
     3181 cgcggcgaac ttcgtggatt cgatcgcgcg tgcgctcgaa gcgtcgggtc taccgcccga
     3241 actgctcgtc atcgaagtga ccgagcgcgt cctcatggac gaccgtgaac gtgccgccga
     3301 gatcctcggc cgccttgccg agttgggtgt gcaactcagc gtcgacgact tcgggacggg
     3361 ccagtcgctg tcgtacttga agcacctgcc ggtgcactcg ctgaagatcg accgctcgtt
     3421 catcgacggg ctcggcgccg acctcgacga cgcggcgatc gtcgccgcga tcgtcgccct
     3481 cggtcacgcg ctcggcctga cggtcaccgc cgaaggcatc gagacgccgc ggcaactgtc
     3541 cgaagtgcgt tcgctcggct gcgacaccgg ccagggctac tacttcgccc gcccccaacc
     3601 gggggaagtc gtccgcgccc tggtccaccg ccagatccgc tggcgcgaac gcgccagctg
     3661 acacgggcgt cgctggaccc caacaattga gggcgagcga cgcccgagtc agctcgtcgt
     3721 gccgcgccac gccttcagca tggcccaggc gtagatgccg gtgtcgtggc tgtcgttcca
     3781 gcgcagggag atgccccacc cgccgaccag ctcggcaccc gtggcttcca gcacgtccgg
     3841 tttcccggcg cggggccaga tcggctcgtg ctgctcgcgc cggccccggc attccgcgca
     3901 cgggcagttc agccgcagct cggcgaggtc gaagcggtct tcggtcccgt cggcccagcg
     3961 gatcgtgagg ccgacccgcc ggtccaggtc gaccgattcc ggcggcggct cttcgggcag
     4021 gtccatgggc acggagctga gtcttcccca gatgggccgc tgcgcttgaa tacctctatg
     4081 gcccgacctt cgcggctgtc cgacgacgac gtgagcgccg cgctcgcgtc ccttccggag
     4141 tgggcgcggg agggcgacga gatcgtcaag acgttcgcct gcgcgtcgtt tcccgatgcg
     4201 atcgcattcg tcgtgcggat cggcttcttc gcggagaagg ccgatcacca tcccgatctc
     4261 gacatccgtt ggcggaacgt gcgcgtcgcg ttgacgacgc acgatgcggg cgggctcacg
     4321 cagctcgatg tcgatctcgc gaccgcgatc gacggcgcct tcgcgggttg agccgtgccc
     4381 ctcgtcatcg cgatcgtgct cgtgttcgtc ttgctcggca ttctctggac cgcggtcgcg
     4441 cgcgagaagg gtccggcgcc acaagacgtc gcgctggcgt acgagaacgc gtgggaccag
     4501 ctcgacttcg acttgctcta cgacctctcc ggtgacgagc tgcgcgacgg actcgggcgc
     4561 aagcagttca tcgcgacgaa gaaggccgcg tacaccgccg accggcacgg gcggttgggt
     4621 gccgaggtcg cggtcgagga atcgatctcg accggtcaga ccgcgctcgt cgtcacgcgc
     4681 gtcacgacgg cggacggaag cgtccgcaac aacgtgatgc tcgagcaccg cccgtccggc
     4741 tggctcgtcg tgaagtactc gatccgcgcg tgacctttcc tggggtggtg ctgacgggcg
     4801 gccgcagcac gcggatgggg cgcgacaagg cgacgatcgt cgtcgacggt gagacgtggg
     4861 cggtgcgatc gggacggctg ctcgcgcagg tatgcgcgcc cgtcatcgag gtcggccccg
     4921 gcgtatcggg actgccggcg atcgtcgaag cgacgccggg acgcggacca ctggtcgcgt
     4981 tcctcgcggg tgtcgacgcg ctcgcggcaa cgggtccggt tgtgttgctc gcgtgcgaca
     5041 tgccgcgtct cgatgtcgcg ctcctcgagc tcgtcgcgaa ctggcccggc accggcacgg
     5101 tcgtgccagt ggtcgacggc cacaagcaac actcgtgctc gcggtggtcg agcgcggcaa
     5161 tcgggcgcgg tcgcaacgcg cccgacggcg cgatcggcgc gatgcgcggg gacgacaccg
     5221 tgttcctcga ggaatccgtg tggcggcgcg tcacgtccgc accggggctc acggacgccg
     5281 acacgcccga ggacctcgct aggttgggcc tcacatgaag gtctcggatc gtgcgattcg
     5341 caagccggtc tcacccgcgc aggtgctgca cgtcggtgcc gacggcgcgc gccgcgcgcc
     5401 cgaccgcctc gtgaccgagg aaccgatgga gatccgggcg cacgggccgg gggaggagcc
     5461 cgcaccagtc gtcgtcacga tgcgcacccc cgggaacgac ttcgagctgg ccgccgggtt
     5521 cttgcatacc gaaggcctgc tgcccgacgc ggccgcgctc gacacgatcg cctactgcct
     5581 cggcggcgaa ggcgagcaga tgtacaacgt cgtgacggtc aaggcgcgcc ggccgatcgt
     5641 cgggcggttg cgcgagcgga acttcctgtc gaccgcgtcg tgcggcatct gcggcaagac
     5701 cgcgctcgac gacgtcgagg tccgttgcgc gcccgtcggt gcggggccga cgatcaccgc
     5761 cgacgtcgtt cgttccttgc cgtccacgct tgccgcgcag caacgcgtgt tcggcgagac
     5821 gggcgggttg cacgcggccg cgacgtttgc gtccgacggt tcgttgctca cgttgcgcga
     5881 agacgtcggc cgccacaacg cgctcgacaa gctcgtcggg catgcgtttc tcgagcgcgc
     5941 gctccctctc gccgaccaag tgctgctcgt ctccggtcga ctctcgttcg agctcgtgca
     6001 aaaggccgca gtcgccggca tacccgtgat ctgcgcggtg tcggcgccgt cgaatctcgc
     6061 ggtcggcgcc gccgagcgct tcgggcagac cgtcgtcggc ttcctgcgcg acgaccgctt
     6121 caacgtctac tgcaatcccg accgggtgtt ggtgtgagca agcgcgacaa ccggatcagg
     6181 acgtcgaaac agcttcggcg cgacctgtgg gtcggctgga agccgaacgg cgtgggggag
     6241 cagaagccgc atcactaccg cgacatgacg cgcaccgtct gggagaaccg caagaacctg
     6301 ccgtacgcgt ggcgcatcct gcgcaaaggc gtgtgcgacg gctgcgcgct cggtgccgcc
     6361 ggcttccacg actggacgat caccggtgtg catctgtgca cgacgcgcct cgagctgctg
     6421 aaggtcaaca ccgcgcccgc gatcgccgac gg
//
LOCUS       DASUGA010000425         2258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1410886, whole genome shotgun sequence.
ACCESSION   DASUGA010000425 DASUGA010000000
VERSION     DASUGA010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410886"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..2126)
                     /locus_tag="VFX21_08440"
     CDS             complement(153..2126)
                     /locus_tag="VFX21_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5096027.1"
                     /translation="MLFTPLRLGPVEARNRVVCGAHFTMYTEPNSTYGEPGFYGERYG
                     RYLSEFARGGAGVVIAGQAQVHPTTAYQMHNNANVWDPACVPHLARVSAQVQEHGALA
                     FLQLAHNGGVNHALWSRLPVWSASGIANHMEAAARIDVAQIDEVVDHFARSARHASSA
                     GFDGIEVHGAHGYLIQEFLSPKSNTRTDEYGGSLENRMRFGVRVLESVRAAVGDRIAV
                     GLRLVGDEEDREGKGLTANDCAEIAAAFAEGGLVDFLDVSIGTSGVGMVRPMYVPHLV
                     GVRATAVVKKAVPDTPVFAVHRILTPDEAEGILERGEADAITIVRALIADPEWANKAR
                     DGRAGEIRKCTGCNQGCYGNLTQGYPILCVTNPVVGREAALGSGTLAPAVTRKRVVVV
                     GGGPAGLEAAWVAAARGHDVVLLERALELGGKIRLAQQLPGRAEIADFADWRAAECAR
                     RGVDIRCGVDADADTVFALAPDAVVIATGGRATVDTPTKSHPMPIAGSDQDWVITHED
                     AVCRAADLGPNIVVVEAVGHIEGIGVGHLLAEQGHNVSVVTPLATPLLLDAETMQKAL
                     PRAVRAGVRWLPNTAVVGIGDHEVTIANVLSYAIETLPADTVVIRTHGVPDDALYDAL
                     RDRVPEVHRVGDAVAVRLADRAISDGHLVGRAL"
BASE COUNT          318 a          810 c          789 g          341 t
ORIGIN      
        1 ccagtcgccg ccgtcgtcgg gccacgacga gatctcgtcg accgcgcgac gcagcgcatc
       61 gacaccggcg tcgtcgagcg tgtcgacgag tacccacccc gtcgcaccga aatgcgcgac
      121 cgcgtcggcc gcgaccgagt cgaatgcgcg gatcacaggg cgcgccccac caggtgaccg
      181 tcggagatcg cgcggtcggc gagccgcacc gcgactgcgt cgcccacgcg gtgcacctcg
      241 ggcacccggt cgcgcagcgc gtcgtacagc gcgtcgtcgg gaacgccgtg cgtgcggatc
      301 accacggtgt cggcgggcag cgtctcgatc gcgtacgaca agacgttcgc gatcgtcacc
      361 tcgtggtcac cgatgccgac gaccgcggtg ttcggcagcc agcgcacacc ggcccgcact
      421 gcgcgcggca gggccttctg catcgtctcg gcgtcgagga gcagtggtgt cgcgagcggt
      481 gtcacgacgc tgacgttgtg tccctgctcg gcaagcaggt gcccgacgcc gatgccttcg
      541 atgtgcccga cggcttcgac gacgacgatg ttcgggccaa ggtccgcggc gcggcacacc
      601 gcgtcctcgt gggtgatcac ccagtcctgg tcgctgcccg cgatcggcat cgggtgcgac
      661 ttcgtcggcg tgtcgaccgt tgcgcgcccg cccgtcgcga tcacgacggc gtcgggtgcg
      721 agcgcgaaca ccgtgtcggc gtcggcgtcg acgccgcaac ggatgtcgac gccgcggcgt
      781 gcgcattcgg cggcccgcca gtcggcgaag tcggcgatct ccgcgcgccc ggggagttgt
      841 tgcgcaaggc ggatcttgcc gccgagctcg agcgcgcgtt cgagaagcac gacgtcgtgc
      901 ccgcgcgcgg cggcgaccca tgctgcttcg agtccggcgg ggccaccgcc gacgaccacc
      961 acccgcttgc gcgtgaccgc aggcgccagg gtgccgctcc cgagcgcggc ttcacgaccg
     1021 acgaccgggt tggtcacgca gaggatcgga tagccctgcg tgaggttgcc gtagcagcct
     1081 tggttgcatc ccgtgcattt gcggatctcg ccggcccggc cgtcgcgcgc cttgttcgcc
     1141 cactcgggat cggcgatgag cgcgcgcacg atcgtgatcg cgtcggcttc accgcgttcg
     1201 aggatgcctt cggcttcgtc gggcgtgagg atgcgatgca ccgcgaacac cggcgtgtcg
     1261 ggcactgcct tcttgacgac ggcggtcgcg cgcacgccga cgagatgggg cacgtacatc
     1321 gggcgcacca tgccgacgcc ggacgtgccg atgctgacgt cgaggaagtc gaccaacccg
     1381 ccctcggcga acgcggctgc gatctcggcg cagtcgttcg cggtgagccc tttgccctcg
     1441 cggtcttcct cgtcgccgac caggcgcaga ccgacggcga tgcgatcacc gaccgcggcg
     1501 cgcaccgact cgagcacccg taccccgaac cgcatgcggt tctcgagcga cccgccgtat
     1561 tcgtcggtcc gcgtgttgct cttgggcgac aagaactcct ggatcaggta gccgtgggca
     1621 ccgtgcactt cgatgccgtc gaagccggcg gacgacgcgt gccttgcgct gcgcgcgaag
     1681 tggtcgacga cctcgtcgat ctgggcgacg tcgatgcgcg ccgcggcctc catgtggttc
     1741 gcgatgccgg acgccgacca gaccggcagc cgcgaccaga gcgcgtggtt gacgccgccg
     1801 ttgtgcgcga gttgcaagaa cgcgagcgcg ccgtgttctt ggacctgcgc actgacccgc
     1861 gcgaggtgcg gcacgcacgc cgggtcccac acgttcgcgt tgttgtgcat ctggtacgcg
     1921 gtcgtcgggt gcacctgcgc ctggcccgcg atcacgacac cggcgccgcc gcgcgcgaac
     1981 tccgacaggt aacgcccgta acgttcgccg tagaaccccg gttcgccgta cgtcgagttc
     2041 ggctcggtgt acatcgtgaa gtgcgcaccg cacacgaccc gattgcgcgc ttcgaccggc
     2101 ccgagacgaa gcggcgtgaa cagcatctcg ggcgcgctag gcgcggctgg ccttgcgcca
     2161 caccgcccgg tacgcgtcga tgccgtcggc cgcgatgaaa cggtcggggc ccgccgccac
     2221 ggacacgcgc tcggggatcg atgcccacac cgggacgc
//
LOCUS       DASUGA010000426         5196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1173166, whole genome shotgun sequence.
ACCESSION   DASUGA010000426 DASUGA010000000
VERSION     DASUGA010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173166"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VFX21_08445"
     CDS             <1..338
                     /locus_tag="VFX21_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5096028.1"
                     /translation="QRVLLARSLMNEPGVVLFDEPTAGLDVGGREELVGDLERWARDP
                     ARPPIVLVTHHLEEIPPGFTHALVLRDGSTLASGPLREAVTSETLSAAFGLRLRVDES
                     DGRYAARLL"
     gene            388..615
                     /locus_tag="VFX21_08450"
     CDS             388..615
                     /locus_tag="VFX21_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008364037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096029.1"
                     /translation="MTDASQLGKQATHADPAIGLAAAAALRKLADQLELAQVRRAREL
                     GWSWREIAHRLDLTKQAVHQRYAALVDPKAG"
     gene            617..1048
                     /locus_tag="VFX21_08455"
     CDS             617..1048
                     /locus_tag="VFX21_08455"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX5096030.1"
                     /translation="MEQLIDRARTEALQLGHNYVGTEHVLLAVTDADPKLLAPYGVSS
                     VDVRKGVMTEITRIVNERAAAKPFVDGVRKSAHEQFGVDAQPPPFAFSSLTPRLAKIL
                     EGAASDQDSDVLAAIIDEDSNLAVIVLERLGVPLDALRASL"
     gene            complement(993..1502)
                     /locus_tag="VFX21_08460"
     CDS             complement(993..1502)
                     /locus_tag="VFX21_08460"
                     /inference="COORDINATES: protein motif:HMM:NF014977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-lytic protease prodomain-containing
                     protein"
                     /protein_id="HEX5096031.1"
                     /translation="MGSEVDVQDGQDEAAFRRTRQLEELRLDISEVDTYAREVDAEFG
                     GSWLVSDADPIRLVLAVTTDPAPHRAAVAGRLEHPDRLDVVQVRYPHAELREIQRAVD
                     ADATGVAAFAAVAIDAPTNAVVVTLGAANEVYADELRLRFGDRVRVEVELTDSPAARP
                     EERRDAPGR"
     gene            complement(1540..2793)
                     /gene="clpX"
                     /locus_tag="VFX21_08465"
     CDS             complement(1540..2793)
                     /gene="clpX"
                     /locus_tag="VFX21_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX5096032.1"
                     /translation="MAKFGDGGDLLKCSFCGKSQKQVKKLIAGPGVYICDECIDLCNE
                     IIEEELSQNTEVRFDDLPKPREIYEYLNDYIIGQETAKKILSVAVYNHYKRVQAGAYG
                     DPDVELQKSNILLIGPTGCGKTLLAQTLARMLKVPFAIADATALTEAGYVGEDVENIL
                     LKLIQAADYDVKKAETGIIYIDEIDKIARKAENPSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPHQEFIQIDTTNILFICGGAFAGLDKIIESRVGNRGVGFGADVRRSDE
                     KDLGELLAGVLPEDLLKFGLIPEFVGRLPVTGVVHNLDQTALMRILVEPKNALVKQYQ
                     KFFHFDDVELVFSDDALESIAEEALKRGTGARGLRAILEEVLLEVMYELPSRTDVTKC
                     VVDREVVMHKVAPTLVSDEEGRRSA"
     gene            complement(2824..3456)
                     /locus_tag="VFX21_08470"
     CDS             complement(2824..3456)
                     /locus_tag="VFX21_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX5096033.1"
                     /translation="MQPINNVYMPYVTQETPRGTETRDVYSRLLKDRIIFLGTPIDDT
                     VANLIIAQLLHLESEDPDKDIMVYINSPGGEITGLFAIYDTMQYIKADVQTICVGQAA
                     SAAAVILAAGEPGKRFILPHSRVLIHQPHGGASGQAVDIEIQAKEIVRMRQLLDEMLA
                     HHTGQALDRVSKDTDRDFIMSGQEAKDYGIVDEVITNRELAAVPSAEGTN"
     gene            complement(3456..4835)
                     /gene="tig"
                     /locus_tag="VFX21_08475"
     CDS             complement(3456..4835)
                     /gene="tig"
                     /locus_tag="VFX21_08475"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX5096034.1"
                     /translation="MQATVESLEGNKVKLHVTVPASEFERAIDSAFRKLAQEVRIPGF
                     RPGKAPRRLLEARFGTDLAREQALRDALPEYYVEAVEANDVDVIAPPEIEIKSGEEEG
                     DVEFEAVVEVRPQVKLLGYDELRVELPVTPIDDEAVEQQVDALRDRFADLADSEYPLI
                     DDAYATIDVNGSIDGEAVEGLTATDFLYRVGSGMVVPELDDQLRGVRPGAILEFDAEL
                     PERFGEHAGQQVKFRVIVKDAKQKVLPDLTDEWASEASEFDTVDELRDDIRTRLDMMQ
                     KLQAQMAVRDKVLEAAADLVPIEAPETLVDSETRRRVEDLAHRLSHQGANLEQYLAAT
                     GKDPQEFIDEIKVGAARAVLADLALRAVMAQEEITATDDELDVEVGRLAERLNEKVNR
                     VRRDLEKRGALEAVRSDVARGKALEFLVEHAIVVDEEGNVLDLALPQESEAAHDTDDN
                     EDEHERSEA"
     gene            4898..>5196
                     /locus_tag="VFX21_08480"
     CDS             4898..>5196
                     /locus_tag="VFX21_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012412325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5096035.1"
                     /translation="MSDLGLTHIALPVHDIDVSIDFYARYAGMEVVHRRDDPGGNRVA
                     WVSDLTRPFVVVLIETVRSYPSLGDPFSHLGLALASREGVDRVCELARAEERAVHG"
BASE COUNT          793 a         1739 c         1697 g          967 t
ORIGIN      
        1 gccagcgggt gctgctggca cggtcgttga tgaacgagcc cggggtcgtg ctcttcgacg
       61 agcccactgc gggcttggac gtcggcggcc gggaagagct cgtcggcgac ctcgagcgct
      121 gggcccgtga ccccgcccgc ccgccgatcg tcctcgtcac ccaccacctc gaagagatcc
      181 cacccgggtt cacccacgcg ctcgtgctgc gggacgggtc gacgcttgcc tcggggccgt
      241 tgcgggaggc agtcacctcg gaaaccctca gcgccgcatt cgggctccgc ctgcgcgtcg
      301 acgaatccga cggtcgttac gccgcacggc tgctgtgact cgtcaagaca tcttgacgga
      361 tcggcgtcgt caacgtatct tgacgatgtg acggacgcat cgcagctcgg gaagcaggcc
      421 acgcacgcgg atcccgcgat cgggctcgcg gccgcggctg cgttgcgcaa gctcgccgat
      481 cagctcgagc tcgcacaggt gcgccgcgcc cgcgagctcg ggtggtcgtg gcgcgagatc
      541 gcgcaccggc tcgacctgac gaagcaagca gtgcaccagc ggtacgccgc gctcgtggat
      601 ccgaaggcag gctgacatgg aacagctcat cgaccgcgcc cgcaccgagg cgctgcagct
      661 cgggcacaac tacgtcggca ccgagcacgt gctcctcgcg gtcacggacg ccgacccgaa
      721 gctgctcgcg ccctacggcg tctcgagtgt cgacgtgcgg aagggtgtca tgaccgagat
      781 cacgcgcatc gtgaacgagc gcgccgcggc caaaccgttc gtcgacggcg tgcgcaagtc
      841 cgcgcacgag cagttcggcg tcgacgcgca gcccccgccg ttcgcgttct cgtcgctcac
      901 gccgaggctg gccaagatcc tcgaaggtgc ggcatccgat caggacagcg acgtgctcgc
      961 cgcgataatc gacgaagact cgaacctcgc ggtcatcgtc ctggagcgtc tcggcgttcc
     1021 tctggacgcg ctgcgggcga gtctgtgagc tcgacctcga cgcgcacgcg gtcaccgaag
     1081 cgcaaccgca actcgtcggc gtagacctcg ttcgcggcgc ccaacgtcac cacgactgca
     1141 ttcgtcggtg cgtcgatggc gacggcggcg aatgcggcga cgccggtggc gtccgcgtcg
     1201 acggcgcgct ggatctcgcg cagctccgcg tgcgggtacc ggacctgcac cacgtcgagc
     1261 cggtccgggt gctccagccg gccggccacg gcggcgcggt gcggcgcggg atcggtggtg
     1321 acggccagca cgagccggat cgggtccgcg tccgagacca gccaggaacc gccgaactcg
     1381 gcgtcgacct cgcgcgcgta cgtatcgact tcggagatgt cgagacgcag ctcttcgagc
     1441 tggcgcgtcc ggcgaaatgc agcctcgtct tgcccgtcct gcacgtcgac ctcactaccc
     1501 acggcgcacc acgcacgaac cgtggcgagc aatggacaac taggcggagc gccggccttc
     1561 ttcgtcgctg acgagcgtcg gcgcgacctt gtgcatgacg acctcgcggt cgacgacgca
     1621 cttggtgacg tcggtgcgcg acggcagctc gtacatcacc tcgagcaaca cctcttcgag
     1681 gatcgcgcgc agaccgcgcg cgcccgtgcc tcgcttcagt gcttcctcgg cgatggactc
     1741 gagcgcgtcg tcgctgaaca ccagctcgac gtcgtcgaag tggaagaact tctggtattg
     1801 cttcaccagc gcgttcttcg gctcgaccag gatgcgcatc agcgcggtct ggtcgaggtt
     1861 gtgcacgacg ccggtgaccg gcagacgacc gacgaactcg gggatgagcc cgaacttcag
     1921 caggtcttcg ggcagcacgc ctgccagcag ctcgccgagg tccttctcgt cggagcgccg
     1981 gacgtcggcg ccgaagccga caccgcggtt gccgacccgg ctctcgatga tcttgtcgag
     2041 gccggcgaac gcaccaccgc agatgaacaa gatgttcgtg gtgtcgatct ggatgaactc
     2101 ttggtggggg tgcttgcgcc caccctgcgg cggcaccgac gcggtcgtgc cttcgaggat
     2161 cttgagcagc gcctgctgca cgccctcacc cgacacgtcg cgcgtgatcg acgggttctc
     2221 ggccttgcgg gcgatcttgt cgatctcgtc gatgtagatg atgccggtct cggccttctt
     2281 gacgtcgtag tcggccgcct ggatcagctt caggaggatg ttctcgacgt cttcgccgac
     2341 gtaacccgcc tcggtgagcg cggtcgcgtc ggcgatcgcg aagggcacct tcaacatgcg
     2401 ggccagcgtc tgcgccagca acgtcttgcc gcagcccgtg gggccgatca gcaggatgtt
     2461 cgacttctgc aactcgacat cggggtcgcc gtacgcgccg gcttgcacgc gcttgtaatg
     2521 gttgtagacc gcgaccgaca ggatcttctt cgcggtctcc tgcccgatga tgtagtcgtt
     2581 caggtactcg tagatctcac ggggcttggg gaggtcgtcg aaccgaacct cggtgttctg
     2641 cgagagctct tcttcgatga tctcgttgca gagatcgatg cactcgtcgc agatgtacac
     2701 accggggccg gcgatcagct tcttgacctg cttttggctc ttcccacaga aggagcactt
     2761 caacaggtct ccgccgtcac cgaacttcgc cacgactcgt ccccctctcc ccgatgcgcc
     2821 tgtttagttc gtgccttcgg ccgacggtac cgcggccagc tcgcggttgg tgatgacttc
     2881 gtcgacgatc ccgtagtcct tggcctcctg gccggacatg atgaagtccc ggtcggtgtc
     2941 cttggacacc cgatcgagtg cttgacccgt gtggtgcgca agcatttcgt cgagcagctg
     3001 acgcatccgc acgatttcct tggcctggat ctcgatgtcg accgcttggc cggacgcacc
     3061 accgtgcggc tggtggatca gcacgcgcga gtgcggaagg atgaaccgct tgcccggttc
     3121 gcccgcggcg aggatgaccg cggccgccga tgcggcctga ccgacgcaga tcgtctgcac
     3181 atcggccttg atgtactgca tcgtgtcgta gatcgcgaac aggcccgtga tctcgccacc
     3241 cggcgagttg atgtacacca tgatgtcctt gtcggggtct tcggactcga ggtgcaacag
     3301 ctgggcgatg atcaggttgg cgaccgtgtc gtcgatgggc gtgcccaaga agatgatgcg
     3361 gtccttgagc agacgcgagt agacgtcacg cgtctcggtg ccgcgaggcg tctcctgtgt
     3421 gacgtacggc atgtacacgt tgttgatggg ctgcattacg cctcggacct ttcgtgttcg
     3481 tcttcgttgt cgtcggtgtc gtgcgcggcc tcggactcct gcggaagcgc gagatcgaga
     3541 acgttgcctt cttcgtcgac cactatcgcg tgctcgacca ggaattcgag tgccttcccc
     3601 cgtgcaacat cagagcgtac cgcctccaac gcgcctctct tttcaaggtc gcgccggacc
     3661 cggttgacct tctcgttcag ccgttcggcc aggcgcccca cctcgacatc gagctcgtcg
     3721 tcggtggcgg taatctcctc ttgggccatc accgcgcgca gcgcgaggtc ggcaagaacc
     3781 gcgcgggccg cgccgacttt gatctcgtcg atgaactcct gcggatcctt gcccgtcgcg
     3841 gcgagatact gctcgaggtt cgcgccctgg tgtgacaacc ggtgcgcgag atcctcgacg
     3901 cgtcgacgcg tctcgctgtc gacgagcgtc tccggcgctt cgatcggcac caggtccgcg
     3961 gcggcttcga gcaccttgtc gcgcaccgcc atctgcgcct gcaacttctg catcatgtcg
     4021 aggcgcgtgc ggatgtcgtc gcgcaactcg tcgacggtgt cgaactcgct cgcttccgac
     4081 gcccactcgt cggtgaggtc gggcaacacc ttctgttttg cgtccttgac gatgacccgg
     4141 aacttcacct gttggcccgc gtgctcgccg aagcgctccg gcaactcggc gtcgaactcg
     4201 aggatcgcgc cgggacgaac gccgcgcagc tggtcgtcga gctcggggac gaccatgccc
     4261 gagccgacgc ggtagaggaa gtcggtggcc gtcaggcctt cgaccgcttc accgtcgatc
     4321 gagccgttca cgtcgatcgt ggcgtacgcg tcgtcgatga gcggatactc ggagtcggcg
     4381 agatcggcga agcggtcgcg taacgcgtcg acctgctgct cgaccgcttc gtcgtcgatc
     4441 ggtgtgaccg gcagctccac gcgcagctcg tcgtagccga gcagcttgac ctgcggccgc
     4501 acttcgacga cggcttcgaa ctcgacgtcg ccttcttctt cacccgactt gatctcgatc
     4561 tcgggcgggg cgatgacgtc gacgtcgttc gcttcgacgg cttcgacgta gtactcgggg
     4621 agcgcgtcgc gcagcgcttg ttcgcgagcg agatcggtgc cgaaccgggc ttcgagcagt
     4681 cgccggggtg ccttgccggg ccggaacccg gggatgcgca cttcttgggc gagcttgcgg
     4741 aacgcgctgt cgatggcccg ctcgaactcc gatgccggca cggtgacgtg aagcttgacc
     4801 ttgttcccct cgagggactc gaccgtggcc tgcataagga agaaggagtg taccggcggg
     4861 ctgtgccatc cccggttcct gcgtacgctc ggtgttcttg agcgacctgg gcctcacaca
     4921 tatcgcactg ccggtgcatg acatcgatgt gagcatcgac ttctacgccc ggtacgccgg
     4981 catggaggtc gtgcaccgcc gcgacgatcc gggcgggaac cgggtcgcgt gggtcagcga
     5041 cctgaccagg cctttcgtcg tggtcttgat cgagaccgtg cggtcctacc cgtcactggg
     5101 cgaccccttc tcgcatctcg gtctcgcgct cgcatcccga gaaggcgtcg accgcgtgtg
     5161 cgaactggcg cgagcagagg aacgcgccgt gcacgg
//
LOCUS       DASUGA010000427         2022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_682876, whole genome shotgun sequence.
ACCESSION   DASUGA010000427 DASUGA010000000
VERSION     DASUGA010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682876"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1692
                     /locus_tag="VFX21_08485"
     CDS             <1..1692
                     /locus_tag="VFX21_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX5096036.1"
                     /translation="EQARPLADARVCLCDSKWRDGDAKPLAAYALARTAGDGVARFAA
                     AAAQLDMVVVLANGRRPVQAERSVVAGEQAVVVDQQLSITGVVLVDGGRPGESIELTA
                     CAGKRGDTWCDAARAALAPFDPDKAAGSAIADAAGRFAFFGLTPQTEYSIQPLVKAFK
                     RPGTESEPAVMATPLAVDVVLQLERMPLIRGRPVLPVGVPSDGREQVWINPRFVMANG
                     WSELGATPCRIGEQFTIAVVDSAYSEVRPDTKTVSTVLEFRFGRGKASPVVATRTVAC
                     PFDGVHDLGNVVLEDPRADREVRIVDRAGAPIAGARVISLGLVSAPAGTDGIVRVAVG
                     PPPATIVAGARGFRIRSVDVTDTSARPLDVVLEPANRLGIEARVGAAAQPRSPMLVSL
                     VFAGMPRDHDFGDRKAVSELRGAKLVMTVARGDEVEYVLELPASGVLDLFDLPSALEF
                     TVKLCDGFGHPIEARSVTMGTDERRDLRFVLAQPPRCLAGQVVDREGQPLRGVEVHVG
                     RSFDELLTDADGRFRVEGVCSDKPSLRFDKVGFRQLMVTGAQLFDRTTFRLERAN"
     gene            complement(1699..>2022)
                     /locus_tag="VFX21_08490"
     CDS             complement(1699..>2022)
                     /locus_tag="VFX21_08490"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX5096037.1"
                     /translation="GGERFQQLRTLLECRVVKSIHTATPVVLAGDFNDVWGTLGPRIL
                     VPAGFRGPHRPLRTFPAWAPVRALDSVYVRGDADVSSLERLRTASARAASDHLPLIAA
                     VDVKA"
BASE COUNT          285 a          675 c          758 g          304 t
ORIGIN      
        1 gaacaggcgc ggccgctcgc tgacgcgcgc gtgtgtttgt gcgactcgaa gtggcgcgac
       61 ggcgacgcga aaccgctcgc ggcgtacgcg ctcgcgcgca ctgccggcga tggcgtcgcg
      121 aggttcgcgg cggcggccgc acaactcgat atggtcgtcg tgctcgcgaa tggcaggcgt
      181 cccgttcagg ctgagcgcag cgtggtcgcg ggggaacagg ctgtcgtcgt cgaccagcaa
      241 ctgtcgatca ccggcgtcgt gctcgtggac ggcggtcgcc ccggcgagtc gatcgagctc
      301 acagcctgcg ccggcaagcg cggcgacacg tggtgcgacg cggcgcgggc ggcgctggct
      361 ccgttcgatc ccgacaaggc cgcgggcagc gccattgccg acgcggccgg ccgcttcgcg
      421 ttcttcggcc tcacgccaca gaccgagtac tcgatccagc cgctggtcaa ggcgttcaag
      481 cggcccggca ccgagtcgga gccagcggtg atggcgacgc cgctcgcggt cgacgtggtg
      541 ctgcagctcg aacgcatgcc gttgatccgc ggccggcccg tgctgccggt cggtgtgccg
      601 tccgacggcc gcgaacaggt ctggatcaac ccgcgcttcg tgatggcgaa cggttggtcc
      661 gaactgggcg ccacgccgtg ccggatcggc gaacagttca cgatcgccgt cgtcgacagc
      721 gcgtactcgg aggtgcgccc cgacaccaag accgtcagca ctgtcctcga gttccgcttc
      781 ggtcggggca aggcgagtcc ggtcgtggcg acgcgcaccg tggcatgtcc attcgacggt
      841 gtgcacgacc tcggcaatgt cgtgctcgag gatccgcggg cggatcgcga ggtgcggatc
      901 gtcgaccgcg cgggcgctcc gatcgcgggc gcgcgcgtca tctcgctggg tctggtcagt
      961 gcacccgcgg gcacggacgg gatcgtgcgc gtcgccgtcg ggccgccgcc tgcgacgatc
     1021 gtcgcgggtg cacgaggctt ccgcatccga tcggtcgacg tgaccgacac gtcggctcga
     1081 ccgctggacg tcgtgctcga gccggcgaac cgactgggca tcgaagcgcg cgtgggagcg
     1141 gctgcgcagc cacgatctcc gatgctggta tcgctggtgt tcgcgggcat gccgcgcgat
     1201 cacgacttcg gggaccgcaa ggcggtcagt gagctgcgcg gtgcgaagct ggtcatgacc
     1261 gtcgctcgcg gggacgaggt cgagtacgtc ctcgaattgc cagccagtgg agtgctggac
     1321 ctgttcgatc tgccgtcggc gctcgaattc accgtgaagc tgtgcgacgg cttcggccat
     1381 ccgatcgaag cgcgcagcgt cacgatgggc acggacgagc ggcgcgacct gcgcttcgtc
     1441 ctagcgcagc caccgcgttg cctcgccggc caggtcgtgg accgcgaggg ccagccgctg
     1501 cgcggcgtcg aggtgcacgt gggtaggtcg ttcgacgagc tgctcacgga cgccgacgga
     1561 aggttccgcg tcgagggggt atgcagcgac aagccgagtc tgcgcttcga caaggtcggc
     1621 ttccgccagc tcatggtgac cggcgcccag ttgttcgatc ggacgacgtt ccgcctcgag
     1681 cgcgcgaact gacgcgcttc acgccttgac gtcgacggcg gcgatcagcg gcaggtggtc
     1741 cgacgcggcg cgggcgctgg cggtgcgcag gcgttcgagc gacgacacat cggcgtcgcc
     1801 gcgcacgtag accgagtcga gcgcacgcac cggcgcccac gcggggaacg tgcgcagcgg
     1861 gcggtgcgga ccgcggaagc cggcgggcac gaggatgcgc gggccgagcg tgccccacac
     1921 gtcgttgaag tcgccggcga gcacgaccgg cgtcgcggtg tggatgctct tcacgacgcg
     1981 gcactcgagc agcgtgcgca gctgctggaa gcgctcgccg cc
//
LOCUS       DASUGA010000428         2519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1270918, whole genome shotgun sequence.
ACCESSION   DASUGA010000428 DASUGA010000000
VERSION     DASUGA010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270918"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2519
                     /gene="rpoC"
                     /locus_tag="VFX21_08495"
     CDS             <1..>2519
                     /gene="rpoC"
                     /locus_tag="VFX21_08495"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX5096038.1"
                     /translation="LDVVPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRL
                     LDLGAPEIIINNEKRMLQEAVDALFDNGRRGRPVTGPGNRALKSLSDMLKGKQGRFRQ
                     NLLGKRVDYSGRSVIVVGPMLRLQQCGLPKQMALELFKPFVMKRLVDLEYAQNIKSAK
                     RMVERARPQVWDVLEEVIKEHPVFLNRAPTLHRLGIQAFEPVLVEGKAIQIHPLVCAA
                     FNADFDGDQMAVHLPLSSEAQAEARMLMLAAHNILSPASGRPIAVPSQDMIIGLYYLS
                     EIFDGQKGEGRVFRSLDEAFAAYEERWGGDGETVSAHAKVKVRLPDGKFPADKFARPV
                     VNESPDGGTTYTPQSLVLREYPDANEVLVETSLGRLLLNSAYPDDFPYVDRVMKKRDI
                     TEVVGELVVSYDKAVVANSLDRMKGLGYEWSTRAGLTISIADVTTPPAKAGLLEKFEG
                     EAAKVESQYERGIITDDERRQQEIEIWTDATNQVTKAMQDLLMSKPFNPIDMMVNSGA
                     RGNVMQVRQIAGMRGLVANPRGEIIPRPIKSNFREGLSVLEYFISTHGARKGLADTAL
                     RTADSGYLTRRLVDVAQELIVREIDCESNRGIWIEDVHGDAESYRVLETSLLGRCIAE
                     DLTLVDGTVIPRNTEIDDPDLRRIADDEGITRVRVRSVLTCETVAGVCASCYGRMLAT
                     GKFSDLGEAIGIVAAQSIGEPGTQLTMRTFHTGGVAGEDITHGLPRVVELFEARTPKS
                     AALMADVSGVARIGENDKGERVITIVADDGTEFEYAVSRRAHISVRDGQEVAAGDRLI
                     GDEKSPLDPKKLLEIKGVRETQQYLVDEVQRVYRDQGVSIH"
BASE COUNT          452 a          806 c          825 g          436 t
ORIGIN      
        1 tcctcgacgt cgtgccggtc atcccgcccg acctgcgccc gatggtgcag ctcgacggtg
       61 gccgcttcgc gacctccgac ctcaacgacc tgtaccgccg cgtgatcaac cgcaacaacc
      121 ggttgaagcg actgctcgac ctcggtgctc ccgagatcat catcaacaac gagaagcgca
      181 tgttgcagga ggccgtcgac gcgttgttcg acaacggccg ccgcgggcgt ccggttacgg
      241 gccccggcaa ccgcgcgctc aagtcgctct ccgacatgtt gaagggcaag cagggccggt
      301 tccgtcagaa cctgctcggc aagcgcgtcg actactcggg tcgttccgtc atcgtcgtcg
      361 gtccgatgct gcgcctgcag cagtgcggtc tgccgaagca gatggcgctc gagttgttca
      421 agccgttcgt catgaagcgg ctcgtcgatc tcgagtacgc ccagaacatc aagtccgcca
      481 agcggatggt ggaacgcgcg cgtccgcagg tgtgggacgt gctcgaagaa gtcatcaagg
      541 aacaccccgt gttcctgaac cgtgcaccaa cgctgcaccg tctcggcatt caggccttcg
      601 agcccgtgct cgtcgagggc aaggcgattc agattcaccc gctcgtctgc gcggcgttca
      661 acgccgactt cgacggcgac cagatggccg tgcaccttcc gctttcgtcg gaggcgcagg
      721 ccgaggcgcg catgctcatg ctcgccgcgc acaacatctt gtcgcccgcg tcgggtcgtc
      781 cgattgccgt gccgagccag gacatgatca tcggcctcta ctacctgtcg gagatcttcg
      841 acgggcagaa gggtgaaggc cgcgtgttcc gttcgctcga cgaggcgttc gccgcgtacg
      901 aagagcgttg gggtggcgat ggcgagaccg tgtcggcgca cgccaaggtc aaggtgcgtc
      961 tgcccgacgg caagttcccg gccgacaagt tcgcgcgtcc ggtcgtgaac gagtcgcccg
     1021 acggtgggac gacctacacg ccgcagagcc tcgtgctgcg cgagtacccc gacgcgaacg
     1081 aggtgctcgt cgagacgtcg ctcggccggt tgctgctgaa cagcgcctac ccggacgact
     1141 tcccctacgt cgaccgcgtc atgaagaagc gcgacatcac cgaggttgtc ggtgagctcg
     1201 ttgtttcata cgacaaggcc gtcgtggcga actcgctcga ccgaatgaag ggcctcggct
     1261 acgagtggtc gacccgcgcg ggtctgacca tttcgatcgc cgacgtcaca acgccgccgg
     1321 cgaaggccgg gctgctcgag aagttcgaag gcgaagcggc caaggtcgaa agccagtacg
     1381 agcgcggcat catcaccgac gacgagcgtc ggcagcagga aatcgaaatc tggaccgacg
     1441 cgaccaacca ggtcacgaag gccatgcagg acttgctgat gtcgaagccg ttcaacccga
     1501 tcgacatgat ggtcaactcg ggtgctcgtg gaaacgtcat gcaggtgcgt cagatcgccg
     1561 gtatgcgtgg tctggtggcc aacccgcgtg gtgagatcat cccgcggccg atcaagagca
     1621 acttccgtga aggcctctcc gtgctcgagt acttcatctc gacgcacggt gctcgcaagg
     1681 gtctggccga cacggcgctg cgaaccgccg actccgggta cctgacccgt cgtctcgtcg
     1741 acgtcgcgca ggaactcatc gtgcgggaga tcgactgcga gtcgaaccgt ggcatctgga
     1801 tcgaggacgt gcacggcgac gctgagtcgt atcgcgtcct cgagacgagc ctgctgggcc
     1861 gttgcattgc cgaagacctc acgttggtcg acggcacggt gatcccgcgc aacaccgaga
     1921 tcgacgatcc cgacctgcgc cgtatcgccg acgacgaagg catcacgcgt gtgcgtgtgc
     1981 gttcggtgct cacgtgcgag acggtggccg gcgtttgtgc gtcgtgctac ggccgcatgc
     2041 tcgcgaccgg caagttctcc gatctcggtg aggcgatcgg tatcgtcgcc gcccagtcga
     2101 tcggtgagcc cggcacgcag ctgaccatgc gtacgttcca caccggtggt gttgccggtg
     2161 aggacatcac gcacggtctg cctcgtgtgg tcgagctgtt cgaagcccgt acgccgaaga
     2221 gcgcggcgtt gatggccgac gtgtccggtg tcgctcgcat cggcgagaac gacaagggcg
     2281 agcgcgtcat cacgatcgtt gccgacgacg gcacggagtt cgaatacgcc gtttcccgcc
     2341 gggcgcacat cagcgtgcgt gacggccagg aagttgcggc cggcgaccgg ctgatcggtg
     2401 acgagaagtc gccgctcgac ccgaagaagc tgctcgagat caagggtgtc cgcgagacgc
     2461 agcagtactt ggtcgacgaa gtgcagcggg tctaccgcga ccagggtgtg tcgatccac
//
LOCUS       DASUGA010000429         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_445441, whole genome shotgun sequence.
ACCESSION   DASUGA010000429 DASUGA010000000
VERSION     DASUGA010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445441"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /gene="sbcD"
                     /locus_tag="VFX21_08500"
     CDS             complement(<1..983)
                     /gene="sbcD"
                     /locus_tag="VFX21_08500"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HEX5096039.1"
                     /translation="MKILHTSDWHVGKLLRGHSRLDEHRAVLDEIVTVAGEEAVDLVL
                     VTGDLFDSAAPPPDAQRVVWDALLALRATGAQVVAIGGNHDNQHAFDAWAPVFAAAGV
                     TLLGHATRPADGGVVEIAAAGERAVCVLVPFVSQRYAVRTEQLLDLDAADASDLYTER
                     MRLLIETLCRAFRPDAVNLLAVHGFVRGGHLGGGERDAHTVFEYGIEGAHFPAGASYV
                     ALGHLHHTQHMAAPAPAWYAGSPIQVDFGEQSERKHVLLVDAAPGVPVKVRKHALTTG
                     WTLRTVRGTVAELADAASSYGDAWLRVVVTEPARAGLADEVRAVLPRAVDIR"
     gene            complement(980..>2062)
                     /locus_tag="VFX21_08505"
     CDS             complement(980..>2062)
                     /locus_tag="VFX21_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5096040.1"
                     /translation="DDTQRDRYRTIGLVPGAFKSVDVYAPPRKFDPNAGPDVATRTQG
                     VHSFYWTIADFVEGDLLPFAFADAEDDRQQYTMVVHNVTAALRKHGERADDGAWRIEG
                     QTLRTYRHLVDFVVDKVNDEDERREWAGGATGSGTVNAFVRRLLSSQRALDRIVRADV
                     VERERHLIETDEAQVTVVDLHNLSDRAKRFVVGVTLRQAFERKEQTGTAKPLLFVVLD
                     ELNKYAPRDGDSPIKEILLDVAERGRSLGIVLIGAQQTASEVERRVIANSSVRVVGRL
                     DPAEAARPEYGFLPATHQKRATIARPGTMFVMQPEIPVPLVAEFPFPAWATRPSERAG
                     PDPTATGAARAPDDVFRGLPGSSSAP"
BASE COUNT          290 a          720 c          752 g          300 t
ORIGIN      
        1 cggatgtcga ccgcgcgcgg caggaccgcg cgcacctcgt cggcgagacc ggcgcgcgcc
       61 ggctcggtga cgacgacgcg gagccacgcg tcgccgtacg acgatgcagc gtccgcgagc
      121 tcggcgacgg tgccccgcac cgtgcgcagc gtccatccgg tggtcagcgc gtgcttgcgg
      181 accttcaccg gaacgccggg tgccgcgtcg acgagcagca cgtgcttgcg ctccgactgc
      241 tcgccgaagt cgacctggat cggtgatccg gcataccacg cgggtgcggg cgcggccatg
      301 tgctgcgtgt ggtgcaggtg gccgagcgcg acgtagctcg cgccggcagg gaagtgggcg
      361 ccctcgatgc cgtactcgaa cacagtgtgc gcgtcgcgct cgccgccgcc gaggtgcccg
      421 ccgcgcacga acccgtgcac cgcgagcagg ttgaccgcat cgggccgaaa ggcacggcag
      481 agcgtctcga tcagcagccg catgcgctcg gtgtagagat ctgatgcgtc ggccgcgtcg
      541 agatcgagga gttgctcggt gcgcaccgca tagcgctggg acacgaacgg caccagcacg
      601 cacactgcgc gttcgccggc cgccgcgatc tcgacgacgc cgccatcggc cgggcgcgtc
      661 gcgtgcccga gcagcgtgac gccggccgcc gcgaacacgg gtgcccacgc gtcgaacgca
      721 tgctggttgt cgtggttgcc gccgatcgcc acgacctgcg cgccggtcgc ccgcagcgcg
      781 agcagtgcgt cccacaccac tcgttgtgca tcgggcggcg gcgcggcgga atcgaagagg
      841 tcgccggtga cgagcacgag gtcgacggcc tcctcacccg cgacggtgac gatctcgtcg
      901 agcacggcgc ggtgctcgtc gaggcgcgag tgaccgcgca gcagcttgcc gacgtgccag
      961 tcggaggtgt ggaggatctt cacggcgcgc tcgacgagcc cggcagcccg cggaacacgt
     1021 cgtcgggcgc tcgggccgca ccggttgcgg tcgggtcggg cccggcccgc tcggacgggc
     1081 gcgtcgccca cgcggggaac gggaactcgg cgaccagcgg caccgggatc tccggttgca
     1141 tcacgaacat cgtccccggg cgggcgatcg tcgcccgctt ctgatgggtc gcgggtagga
     1201 acccgtattc gggacgagcc gcctccgcgg gatcgaggcg gccgacgacc cgcaccgacg
     1261 agttcgcgat cacgcgccgt tcgacttcgc tcgcggtctg ctgggcgccg atcagcacga
     1321 tgccgagcga acggccccgc tcggccacgt cgaggaggat ctccttgatg ggagagtcgc
     1381 cgtcgcgcgg cgcgtacttg ttgagctcgt cgagcacgac gaacagcagc ggcttcgcgg
     1441 tgccggtctg ctccttgcgc tcgaaggcct ggcgcaacgt gacgcccacg acgaagcgct
     1501 tggcccggtc ggagaggttg tggaggtcga cgaccgtgac ctgcgcctcg tcggtctcga
     1561 tcaggtgccg ttcgcgctcg acgacgtcgg cccgcacgat ccggtcgagc gcacgctgcg
     1621 acgagagcag ccggcggacg aacgcgttga cggtgccgct cccggttgcc ccaccggccc
     1681 actcgcgccg ttcgtcttcg tcgttcacct tgtcgacgac gaagtcgacg agatgccggt
     1741 aggtacgcag cgtctgcccc tcgatgcgcc acgcgccgtc gtcggcgcgc tcgccgtgct
     1801 tgcgcaacgc cgcggtcacg ttgtgcacga ccatcgtgta ctgctggcgg tcgtcttccg
     1861 cgtcggcgaa cgcgaacggg aggaggtcgc cttcgacgaa gtcggcgatc gtccaataga
     1921 agctgtgcac gccctgcgta cgggtcgcga cgtcggggcc ggcgttcgga tcgaacttgc
     1981 ggggcggcgc gtacacgtcg accgacttga acgcgcccgg cacgagcccg atcgtgcggt
     2041 agcggtcgcg ctgcgtgtcg tc
//
LOCUS       DASUGA010000430         4099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_855263, whole genome shotgun sequence.
ACCESSION   DASUGA010000430 DASUGA010000000
VERSION     DASUGA010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855263"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..3716
                     /gene="metH"
                     /locus_tag="VFX21_08510"
     CDS             201..3716
                     /gene="metH"
                     /locus_tag="VFX21_08510"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX5096041.1"
                     /translation="MADPRGSIPSVGSGAEHPFLNLLQERVLVFDGAFGTWMQGHDLG
                     PDDFGGLALEGCNEMLVLTRPDLVAQMHAEYFAAGVDAVETATFGAFPLVLNEYQIPE
                     KTFEINKRAAEIATEVARDHSTPDQPRYVIGSMGPGTKLPSIGHIRYEQLRDDYQAQV
                     EGLLAGHVDVLLIETVYDLLQAKAAINGARRAMAAAGTLVPIMVQVTVETTGRMLVGT
                     EIGAALTALEAMQPDVIGMNCATGPAEMTEHLRYLAQHARTFLSCLPNAGLPSVVDGH
                     THYDLTPELLADAHERFVTDFGLNIVGGCCGTTPAHLSAVTERLATPVGRAPVVRVPE
                     FEAGCSSIYSHVPFHQELAYLAIGERTNANGSKKFREAMLEADWDTCVQMAREQVKEG
                     AHVLDVCVDYVGRDGTIDMDEVAGRFATQASLPLVFDSTEPPVVETGLQHHGGKAILN
                     SANLEEGDAEGKRMDRVFRLAREYGAAVICLTIDEEGQARDADWKLRVAHRLYDIATQ
                     RYGIDPHDLIFDPLTFPLSTGDEDLRRDAMESINAIRRIKEELPGASTVLGLSNVSFG
                     LKPAARHVLNSVFLHECVEAGLDAAIVHAARIMPMHKIDERQREVALDLIYDRRRDGY
                     DPLTEFMALFEGVEASAVEREDRSGWPVAERLKHRIIDGDRDGLEADLDEQLETMPAL
                     GIINDVLLDGMKVVGDLFGRGEMQLPFVLQSAETMKASVAHLEPHMEKADAGGKGIVV
                     LATVRGDVHDIGKNLVDIILTNNGYTVHNLGIKIPINEMIDKALEVGADAIGMSGLLV
                     KSTIIMRENLEELNERGLADNMPILLGGAALTRTYVEVDLRQTYEGRVFYGKDAFEGL
                     RTMDALMQGMKSGTLEPGFGRELGGRKLPPRKSEREVEAVEVPARSDVDPEAHVFTPP
                     FIGSRVAKGVPIQDIAAYVNETALFRNQWQFRPEKGGETDEEFKARIRPVLREQLAVA
                     LAEGLLVPAVVYGYFSVNADGNDLVVWKDDSRSQEWLRFNFPRQRKAPFHSIADFFRP
                     LDSGDADYAAFSICTMGRRASEREQQLFADNKYQDYLLLHGLGVEMTEALAELWHRRI
                     REEWGYADEDGPTLAGLFRQQYRGSRYSWGYPACPDLDDQAKVVELLEADRIDVALTE
                     EFHLVPEQSTSAIVVPHPEAKYFVV"
     gene            3776..>4099
                     /locus_tag="VFX21_08515"
     CDS             3776..>4099
                     /locus_tag="VFX21_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096042.1"
                     /translation="MPRPAPGRQHADKTAVARALSAVVGRKAVGAKPDDPDLIVTLPD
                     GRTLAIEVKSVSRAEPGPIANALRTWEPDLRRRRGAHDAAVVGIVVASSVAEATKNIL
                     REHGWG"
BASE COUNT          739 a         1395 c         1358 g          607 t
ORIGIN      
        1 atgggccggc agcgccggca acgcggcgac gagcacagca cagctcgccg cgaaagcaat
       61 tcgtcgaaac attggtcctc ccccacgacc ccacgaatgc ggacagcgca aacataggcg
      121 cccgtggtga acctgaacca acgcgttgat ggttcgaaaa cgaatcccga accatcggag
      181 cgggcacgta cgctgtagga atggccgacc ccagaggctc aattccgtcc gtcggaagtg
      241 gtgccgaaca cccctttctc aatcttttac aagagcgcgt tttggtgttc gacggcgcgt
      301 tcggcacgtg gatgcagggg cacgacctcg gccccgacga cttcggcggc ctggccctcg
      361 aaggctgcaa cgagatgctc gtgctcaccc ggcccgacct cgtcgcgcag atgcacgcgg
      421 agtacttcgc ggcgggcgtc gacgcggtcg agaccgcgac gttcggcgcc ttcccgctgg
      481 tgctcaacga gtaccagatc cccgagaaga cgttcgagat caacaagcga gcggccgaga
      541 tcgcgaccga ggtcgcgcgc gaccactcca cgcccgatca gccgcggtat gtgatcggct
      601 cgatgggccc cggcacgaag ctgccgtcca tcggccacat ccgctacgag cagctgcgcg
      661 acgactacca ggcgcaggtc gaaggcctcc tcgccgggca cgtcgacgtg ttgctgatcg
      721 agacggtgta cgacctgttg caggccaagg ccgcgatcaa cggggcgcgg cgcgcgatgg
      781 cagccgcggg aacgctcgtg ccgatcatgg tgcaggtgac ggtcgagacg accggccgca
      841 tgctcgtcgg caccgagatc ggcgctgccc tcaccgcgct ggaagcgatg cagcccgacg
      901 tcatcggcat gaactgtgcg acgggtcccg cggagatgac cgagcacctg cgctacctcg
      961 cgcagcacgc gcgcacgttc ttgtcgtgcc tgccgaacgc gggcctgccg tcggtcgtcg
     1021 acggacacac gcactacgac ctcacgcccg agttgttggc cgacgcgcac gagcgcttcg
     1081 tcaccgactt cggcctgaac atcgtcggtg gttgctgcgg cacgacaccc gcccacctgt
     1141 cggccgtcac cgaacgcctc gcgacgccgg tggggcgcgc gcccgtcgtg cgcgtgcccg
     1201 agttcgaggc cggctgctcg tcgatctatt cgcacgtacc gttccaccag gagctcgcgt
     1261 acctcgcgat cggcgagcgc accaacgcca acggctcgaa gaagtttcgc gaggcgatgc
     1321 tcgaagccga ctgggacacg tgcgtgcaga tggcgcgcga gcaagtgaag gaaggcgcac
     1381 acgtgctcga cgtgtgtgtc gactacgtcg ggcgcgacgg caccatcgac atggacgaag
     1441 tcgccggccg cttcgcgacg caggcctcgc tgccgctcgt gttcgactcc accgaaccgc
     1501 ccgtcgtcga gaccgggctc cagcaccacg gcggcaaggc gatcctcaac tcggccaacc
     1561 tcgaagaagg tgacgccgag ggcaagcgca tggaccgcgt gttccggctc gcgcgcgagt
     1621 acggcgcggc agtcatctgc ctcaccatcg acgaagaggg ccaggcccgc gacgccgact
     1681 ggaaactgcg ggtcgcgcac cgcctctacg acatcgcgac gcagcggtac ggcatcgatc
     1741 cgcacgacct gatcttcgac ccgctcacgt tcccgttgtc gaccggtgac gaagacctgc
     1801 ggcgcgacgc gatggagtcg atcaacgcga tccgccgcat caaggaagag ctgccggggg
     1861 cgtcgacggt gctcggcttg tcgaacgtgt cgttcggcct caaacccgcg gcgcgccacg
     1921 tgctgaacag cgtgttcctg cacgagtgcg tcgaggccgg cctcgacgcc gcgatcgtgc
     1981 acgcggcgcg catcatgccg atgcacaaga tcgacgagcg ccagcgcgaa gtcgcgctcg
     2041 atctcatcta cgaccgccgg cgcgacggct acgacccgct caccgagttc atggcgctgt
     2101 tcgaaggcgt cgaggcctcc gcggtcgaac gcgaggaccg gtcgggctgg cccgtcgcgg
     2161 agcggttgaa gcaccgcatc atcgacggcg accgcgacgg cctcgaagcc gacctcgacg
     2221 agcaactcga gacgatgccc gcgctcggga tcatcaacga cgtgctgctc gacggcatga
     2281 aggtcgtcgg cgacctgttc ggtcgcggtg agatgcagct gccgttcgtg ttgcagtccg
     2341 cggagacgat gaaggcgtcc gtcgcgcacc tcgaaccgca catggagaaa gccgacgcgg
     2401 gcggcaaagg cattgtcgtg ctcgcaaccg tgcgcggcga cgtccacgac atcggcaaga
     2461 acctcgtcga catcatcctc accaacaacg gctacacggt gcacaacctc gggatcaaga
     2521 tcccgatcaa cgagatgatc gacaaggcgt tggaggtcgg tgcggacgcg atcggtatga
     2581 gcggcctgct cgtgaagagc acgatcatca tgcgcgagaa cctcgaggag ctgaacgagc
     2641 gcgggctcgc cgacaacatg cccatcctcc tcggcggcgc cgcgttgaca cgcacctacg
     2701 tcgaagtcga cctgcgtcag acgtacgaag gccgcgtctt ctacggcaag gacgcgttcg
     2761 agggtctgcg caccatggac gcgctcatgc agggcatgaa gagcggcacg ctcgaacccg
     2821 ggttcgggcg ggagctcggt ggccgcaagc tgccgccgcg caagagcgaa cgcgaggtcg
     2881 aagcggtcga ggtccccgcg cgctccgacg tcgacccaga ggcgcacgtc ttcacgccgc
     2941 cgttcatcgg atcgcgcgtc gccaagggtg tgccgatcca ggacatcgcg gcgtacgtga
     3001 acgagaccgc gctgttccgc aaccagtggc agttccggcc cgagaagggc ggcgagaccg
     3061 acgaggagtt caaggcacgg atccggccgg tgttgcgcga gcagctcgcg gttgcgctgg
     3121 ctgaaggtct gttggtgccg gccgtcgtgt acggctactt ctcggtcaat gccgacggca
     3181 acgacctggt cgtgtggaag gacgactcac gcagccagga gtggttgcgg ttcaacttcc
     3241 cgcggcaacg caaggcgccg ttccactcga tcgccgactt cttccggccg ctcgactcgg
     3301 gtgacgcgga ttacgcggcg ttctcgatct gcacgatggg ccgccgcgcg agcgaacgtg
     3361 agcagcagct gttcgcggac aacaagtacc aggactactt gttgttgcac ggtctcggtg
     3421 tcgagatgac cgaggcgctc gcggagctgt ggcaccgccg catccgcgag gagtggggct
     3481 acgccgacga agacggcccg accctcgccg gcctgttccg ccagcagtac cgcggctcgc
     3541 gctactcgtg gggttacccc gcctgcccgg acctcgacga ccaggccaag gtcgtggagc
     3601 tgctcgaagc cgaccgtatc gacgtcgcgc tgacggagga gttccacctc gtgccggagc
     3661 agtcgacgtc cgcgatcgtc gtcccccacc ccgaggcgaa gtacttcgtc gtctagccgc
     3721 ggcgactgtt cggtgttcgg ttgctaccga acaccgaaca cggtagcctg gatgcgtgcc
     3781 cagaccagcc cccggccggc agcacgccga caagaccgcc gtcgcccgcg cgctcagcgc
     3841 ggtcgtcggc aggaaggccg tcggggcgaa gcccgacgac cccgacctga tcgtcacgct
     3901 ccccgatgga cggacactcg cgatcgaggt caagtccgtt tcgagagccg aacccggacc
     3961 gatcgccaac gcactgcgca cctgggaacc tgacctgcgc cgccgtcgtg gcgcgcacga
     4021 cgccgccgtg gttgggatcg tcgtcgcgag ctcggtcgcc gaagcaacga agaacatcct
     4081 gcgcgagcac ggttgggga
//
LOCUS       DASUGA010000431         2576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_429481, whole genome shotgun sequence.
ACCESSION   DASUGA010000431 DASUGA010000000
VERSION     DASUGA010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429481"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..322
                     /locus_tag="VFX21_08520"
     CDS             74..322
                     /locus_tag="VFX21_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096043.1"
                     /translation="MLLDLNLPPIDSWLVLAALGDRPERPRIVVAVGDRGDAQRAHAL
                     GADVCVVAGTTGPARALSTAWQRHHETISRPTRSFASG"
     gene            265..681
                     /gene="msrB"
                     /locus_tag="VFX21_08525"
     CDS             265..681
                     /gene="msrB"
                     /locus_tag="VFX21_08525"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX5096044.1"
                     /translation="MAKTSRDNLPTDAELRERLTPLQYEVTQKAGTERAFTGDYWDCH
                     DDGTYVCVVCGEPLFSSETKFESGTGWPSFFQPLAEEAVEEANDSSHGMVRTEARCGN
                     CGAHLGHVFPDGPRPTGLRYCMNSASLRLEEHESDT"
     gene            760..1512
                     /locus_tag="VFX21_08530"
     CDS             760..1512
                     /locus_tag="VFX21_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096045.1"
                     /translation="MTALTVGVTTAKQPECKRGIAANFAAALARHSAVSARVCVVDAD
                     PMALDVSTRLGVRGPYLEDFAKASMPQTAQLGRLHSPAMTVVPSNGSPVARVHLAIEK
                     ALPELKEAHDVVVCDLPGGPTGPGLVLGTRLELLDWLVLAVTPNNASIAAANHFLEHF
                     ETAKDRGDIGDVQLAIVATGDESSMLFEAQDVELMLGVNVAGRIPQLWGRAEPNFGFG
                     PALAIPELDDAVYELLMQMRLGHTHRSELITL"
     gene            1683..1964
                     /locus_tag="VFX21_08535"
     CDS             1683..1964
                     /locus_tag="VFX21_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX5096046.1"
                     /translation="MREMLIQLDVDAADRRWQERANCLGVDPDLFFPERGASTKEAKG
                     VCGGCEVRMECLEYALRHGEKFGIWGGMSERERRRIRRQRALARRGLSA"
     gene            complement(1981..>2576)
                     /locus_tag="VFX21_08540"
     CDS             complement(1981..>2576)
                     /locus_tag="VFX21_08540"
                     /inference="COORDINATES: protein motif:HMM:NF014965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096047.1"
                     /translation="ARTFGQWWAEAFEVPVFLYDEASPQKRDLPHARTHGFRSRKPDF
                     GPSEPHPLLGATAVGARKPLVAINCVLVSRDVTIARRIARSMRQAAGPPTGLPGVRAL
                     GFFLGDIGRAQVSMNLFDLDRTGVQDACLHVRELARKLGTDVAAVELVGLLPRRELDR
                     CTDDFLEWSRIDAESSIEARVGHGARWLPGDPPLQPV"
BASE COUNT          419 a          871 c          878 g          408 t
ORIGIN      
        1 tgtcgtcgag caggccgtcg tgaacgacgg tgaagccgcg ctcgccttgc tcgatcgcga
       61 gcgcttcgac gccgtgctgc tcgacctcaa cctgccaccg atcgacagct ggctcgtgct
      121 cgccgccctc ggcgaccgac ccgagcgacc ccggatcgtt gtcgccgtcg gggaccgcgg
      181 cgacgcccag cgggcccacg cactcggcgc cgatgtttgt gtggtggcgg gaaccaccgg
      241 tcccgcgcga gcgttgtcaa cggcatggca aagacatcac gagacaatct cccgaccgac
      301 gcggagcttc gcgagcggct gacccccctt cagtacgagg tcacccaaaa ggccggcacc
      361 gagcgtgcgt tcaccggcga ctactgggac tgtcacgacg acggcaccta tgtgtgtgtc
      421 gtctgcggcg agcccctgtt ctcgtcggag acgaagttcg agtccggcac cggctggccg
      481 agcttcttcc aacccctcgc cgaggaggcc gtggaggaag ccaacgactc cagccacggc
      541 atggtccgca ccgaggcccg gtgcgggaac tgcggcgccc atctcgggca cgtcttcccc
      601 gacggacctc gcccgacggg cctgcgctac tgcatgaatt cggcctcgct ccggctcgag
      661 gagcacgaat cggacaccta ggtccggcgg cccagtgaca tgagccacgg ttggccccgg
      721 aagctgcgga gacgcagccc cggggccgtt acctgtcacg tgacggctct gacggtcggc
      781 gtgacgacgg ccaagcaacc cgagtgcaag cgagggatcg cggcgaattt cgcggcggcg
      841 ctcgcccggc acagcgcggt gtccgctcgg gtgtgcgtgg tcgatgccga cccgatggcc
      901 ctcgacgtgt cgacccggct cggggtacgc gggccctacc tcgaggactt cgccaaagcc
      961 tcgatgcccc agactgccca actcggccgg cttcactcgc ccgcgatgac ggtcgtgccg
     1021 tcgaacggca gcccggttgc ccgagtccac ctcgccatcg agaaggcctt gccggagctg
     1081 aaggaagccc acgacgtggt ggtctgcgac cttcccggcg gtccgaccgg ccccggcctc
     1141 gtgctgggaa cccggctcga gctgctcgac tggctcgtgc tcgcggtgac gccgaacaac
     1201 gcttcgatcg cggccgccaa ccactttctc gaacacttcg aaacagcgaa ggatcgcggc
     1261 gacatcggcg acgtgcagct cgcaatcgtc gccaccggcg acgagagctc gatgctcttc
     1321 gaggcccagg acgtggagct catgctcggc gtgaacgtgg ccggccgcat cccgcagttg
     1381 tggggccgag cggagccgaa cttcggattc ggacctgcgc ttgcgatccc cgagctggac
     1441 gatgcggtct acgaattgct catgcagatg cgactcggac acacgcatcg cagcgaactc
     1501 atcacgctct gagttccttt ctcgcccatt ccggcctttt caagcggaca ttgtgcgcgt
     1561 ccggggcaag tttctgcttg cgccgttgtc aagcactcga caaaatgacg tggctgtaat
     1621 tccatcggcg gcatttaccg tccacagcag cgatcacgac agcgagagcg agggggcagc
     1681 cgatgcgaga gatgttgatt cagctcgacg tcgacgccgc ggaccggcgt tggcaagagc
     1741 gggcgaactg cctcggggtc gaccccgatc tcttctttcc cgaacgcggt gcatctacga
     1801 aagaggcgaa aggcgtgtgc ggcgggtgcg aggtgcgcat ggagtgcctc gaatacgcgc
     1861 ttcgccacgg cgagaagttc ggaatctggg gcgggatgtc ggagcgcgag cggcgccgca
     1921 tccgccgaca gcgggccctt gctcgccgcg gcctgagcgc gtaaccacgc gctcgcacgt
     1981 ctagaccggt tgcaggggcg gatcgcccgg gagccagcgt gcgccgtgac cgactcgcgc
     2041 ctcgatgctc gattcggcgt cgatgcggct ccattcgagg aagtcgtccg tgcagcggtc
     2101 gagctcgcgg cgcggcaaga ggccgacaag ttccacggcc gcgacatcgg tcccgagctt
     2161 gcgagcgagt tcgcgcacgt ggaggcacgc gtcctgcacg ccggtgcggt cgaggtcgaa
     2221 caggttcatc gagacctgcg cccggccgat gtcgccgagg aaaaagccca gcgcccgcac
     2281 gcccggtaat ccggtcggcg ggcccgcggc ttggcgcatc gaacgggcaa tgcggcgcgc
     2341 gatcgtcacg tcgcggctga ccagcacgca gttgatcgcg acgagcggct tgcgcgctcc
     2401 gacggcggtg gcgccgagaa gggggtgcgg ttccgacggc ccgaaatccg gtttgcgcga
     2461 gcggaacccg tgggtgcgcg cgtgcggcag gtcgcgtttt tgggggctgg cttcgtcgta
     2521 caggaagacg gggacctcga acgcttcggc ccaccattgt ccgaacgtgc gcgctt
//
LOCUS       DASUGA010000432         3919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_451363, whole genome shotgun sequence.
ACCESSION   DASUGA010000432 DASUGA010000000
VERSION     DASUGA010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451363"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VFX21_08545"
     CDS             complement(<1..662)
                     /locus_tag="VFX21_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5096048.1"
                     /translation="MLVAEGVRKVYRTGAEEVEALRGVDLVVDDGEFVSIMGPSGSGK
                     TTLLNCCSGLDEIDGGRVLLDGVDLHALSDAERTTNRASSMGFIFQAYNLIPVFSAVE
                     NVELPLLLAGADASDARRRASETLERVGLGHRLQHRPAELSGGEQQRVTVARALAGRP
                     KVVWADEPTGALDSETAGQVMELLRELNADGLTLIVVTHDEGVGRSANRLVGMRDGHI
                     AS"
     gene            complement(693..1127)
                     /locus_tag="VFX21_08550"
     CDS             complement(693..1127)
                     /locus_tag="VFX21_08550"
                     /inference="COORDINATES: protein motif:HMM:NF017426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HEX5096049.1"
                     /translation="MKVHGTEAAAAIVERAKRDRSGALVFTIGTGCCESTAPFLYEDF
                     WPGPDSEQVGEVAGVPVYAPEYLRALYPANDGVTIDVVDDLAESMSIETEYGCRFILR
                     DAAGTAIEGQERQDCEVPEAVSALRSRGPQELPEALRHARMR"
     gene            1187..3253
                     /locus_tag="VFX21_08555"
     CDS             1187..3253
                     /locus_tag="VFX21_08555"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5096050.1"
                     /translation="MSEASEPSGIKRELIAFAELFAMCGFAIAQPLLSVLGQSPDLFI
                     FRDATPGEIIFLAVVIVVAAPLALWVVELLVGLASKRARRVVHLAFLAVLAAIFVVQA
                     VKRATELRGALLVVLAAGAAAGLVTLYTRFKATKTWLLVVSPAPIVFALIFVLSSPVS
                     KIVFPADAQAAVLGSPRNAPSIVMITFDEWPLSSIVGADGKIDATLYPNLAQLAAAST
                     WYRNTTSITNSTWHAIPHLLTGRLGKSNQVPEASYHPENLFTFLGGTYRLRVSETVTR
                     LCPRTLCDTTPAPGAHGLRTLAKDVAKTYRDMISPNDSDAEVTAGFEERTTTAAVDQA
                     KNATGEGDKAAPDLGAATANRPQRFQGFLDGMNPAEPPTLHFLHLLLPHVAYRFLPSG
                     REYSAPADDFGRADDEYNAETWPPQLAHQRLLLQTAYVDRLVGDLLDSLRAKGMYDKT
                     VLVLTADHGITFTPNGPGRGLRDHPLDPKLYPDLLSVPLFVKASGQSRGATSDANVMT
                     IDVVPTIAKLAGFDLPWKVDGVPAGERRDQRKIFRKATVNPFGVEMGPDILYDGAQNE
                     KEMLARNVDTMVKPGDALQFYRVGPHAGLVGTDVADVPHGDRADVEINDPGAYQDVKA
                     APAPLPALLYGNAPRAGDLLVVLNGKVAGVSPTFRDGDQAHRFAVMVPEPLFRTGAND
                     LQLYWL"
     gene            complement(3307..>3919)
                     /locus_tag="VFX21_08560"
     CDS             complement(3307..>3919)
                     /locus_tag="VFX21_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5096051.1"
                     /translation="YDEFVGMLVERAGKIVIGDNMDMNTDLGPLVAPSQVDTTERYVK
                     LGLDEGAKLLCGGAKPANLAAGLDQSAYYLPTIFEADNSMKIAQEEIFGPVLCVIPFD
                     SDDEAIKIANDSIYGLGGGVWSKNLDRAHGVAAAMRTGTVWVNDYHMITPTQPFGGYK
                     QSGIGRELGDYGYNEYRQIKHVWQNTSTDRGGYMHFAALSGNI"
BASE COUNT          667 a         1365 c         1222 g          665 t
ORIGIN      
        1 tcgctggcga tgtgcccgtc gcgcatacca acaagccggt tggcgctgcg gccgacgcct
       61 tcgtcgtgcg tcaccacgat cagcgtgagg ccgtcggcgt tgagctcgcg caacaactcc
      121 atgacctggc cggccgtttc ggagtcgagc gcgccggtcg gttcgtcggc ccacaccacc
      181 ttcggccgcc ccgccaatgc gcgggcaacc gttacccgct gttgttcacc gcccgacaac
      241 tccgccggcc ggtgttgcaa gcggtgaccg agcccgaccc gctcgagcgt ttccgacgcg
      301 cgccgccgtg cgtctgacgc gtccgcgccc gcaaggagga gcgggagttc gacgttctcg
      361 accgcgctga acaccggaat gaggttgtac gcctgaaaga tgaagcccat cgacgacgca
      421 cggttcgtgg tgcgttccgc gtcggaaagc gcgtgcagat cgacaccgtc gagcaggacc
      481 cgcccgccat cgatctcgtc gagcccggaa cagcagttga gcagcgtcgt cttcccggaa
      541 ccggacggtc ccatgatcga gacgaactcg ccgtcgtcca cgacaagatc gacgccccgc
      601 aacgcctcga cttcttccgc gccggtccga tacaccttgc gcacgccctc ggccaccagc
      661 acgccgagaa cgctagggca cgaggggtgc ggtcagcgca tccgtgcgtg tcgcagcgct
      721 tcgggcaatt cttgcggacc gcgggaccgc aatgccgaaa cggcctccgg gacttcgcaa
      781 tcctgccgtt cttggccctc gatcgccgtg ccggcggcat cgcgcaggat gaagcgacag
      841 ccgtattcgg tctcgatcga catcgactcc gcgagatcgt cgacaacgtc gatcgtcacg
      901 ccgtcgttcg cgggatacag cgcgcgcaag tactccggcg cgtacaccgg cacacccgcg
      961 acctcgccga cctgttcgct gtcgggcccg ggccagaaat cttcgtagag gaacggcgcc
     1021 gtcgattcgc aacatcccgt gccgatcgtg aacacgagcg caccggagcg atcgcgtttc
     1081 gcgcgttcga caatcgcagc ggcggcctcg gttccgtgca ccttcacgaa caccacgcta
     1141 cctgccgacc cgaaatccgc cacagtggcc cgatagcctg cgcaatttga gcgaagcgag
     1201 cgaaccgagc ggcatcaagc gcgagctgat cgcgttcgcc gagctgttcg cgatgtgcgg
     1261 attcgcaatc gcccagccgc tgctttccgt gctcggccag agccccgacc tcttcatctt
     1321 ccgggatgcg acgcccggcg aaatcatctt cttggcggtc gtcatcgtcg tcgccgcacc
     1381 gttggcgctc tgggtggtcg agctgctcgt ggggctggcg agcaaacgcg cccggcgcgt
     1441 tgtacatctg gccttcctcg cggtgctcgc cgcgatcttc gtcgtgcagg cggtcaaacg
     1501 cgcgaccgaa ctgcgcggcg cgctcctcgt cgtactcgcg gccggagccg cggccgggct
     1561 cgtgacgctc tacacgcgct tcaaggcaac gaaaacgtgg ttgctcgtcg tctcgcccgc
     1621 gccgatcgtc ttcgcgttga tcttcgtctt gtcgtcaccc gtctcgaaga tcgtgtttcc
     1681 cgccgacgcg caggcggccg tcctcggctc gccccgcaac gcgccctcga tcgtcatgat
     1741 cacgttcgac gagtggccgc tctcgtcaat cgtcggcgcc gacgggaaga tcgacgcgac
     1801 gctctacccc aatctcgcgc agctggcggc ggcatcgacg tggtaccgca acaccaccag
     1861 catcacgaac tcgacgtggc acgcgattcc gcacctcctc accgggcggc tgggcaagag
     1921 taaccaggta ccggaagcgt cgtaccaccc ggaaaatctg ttcacgttcc tcggggggac
     1981 gtaccgattg cgcgtttccg aaaccgtgac acgcctgtgc ccgcgcacgt tgtgcgacac
     2041 gaccccggcg ccgggcgcgc acggactccg cacgctcgcc aaagacgtgg cgaagacgta
     2101 ccgcgacatg atctcgccca acgattccga cgcagaggtc acggccggat tcgaggaacg
     2161 caccacgacc gcggcggtcg accaagcgaa gaacgcgacc ggcgagggcg acaaggcggc
     2221 ccccgatctc ggcgctgcca ccgcgaaccg gccgcagcgc ttccaagggt tcctcgacgg
     2281 catgaacccg gccgagccgc cgacattgca cttcttgcac ttgttgttgc cgcacgtcgc
     2341 ataccgattc ctgccgtccg gtcgcgagta ctccgcgccc gccgacgact tcgggcgcgc
     2401 cgacgacgaa tacaacgccg agacgtggcc gccgcaactc gcgcaccaac gactgctgct
     2461 gcagaccgca tacgtcgacc ggctcgtggg cgatctgctc gactcgctac gcgccaaggg
     2521 catgtacgac aagaccgtgt tggtgctgac cgccgaccac ggcatcacgt tcacgccgaa
     2581 cggtccgggc cgcggcctgc gcgaccatcc gctcgacccg aagctgtatc cagatctgct
     2641 gtcggttccg ctgttcgtca aagccagcgg acaatcgcgt ggcgcgacga gtgacgccaa
     2701 cgtgatgacc atcgacgtcg tgccgacgat cgcgaagctg gccggattcg atctgccgtg
     2761 gaaggtcgac ggcgtgccgg ctggcgagcg ccgagaccag cgaaagatct tccggaaggc
     2821 cacggtgaat ccgttcggcg tcgagatggg ccccgacatc ttgtacgacg gcgcgcagaa
     2881 cgaaaaggaa atgctggcgc gcaacgtcga cacgatggtg aagcccggcg acgcgctcca
     2941 gttctaccgt gtcgggccgc acgccggact cgtgggcaca gacgttgccg acgtgccgca
     3001 cggcgatcgc gccgacgttg agatcaacga tcccggggcg taccaggacg tcaaagccgc
     3061 acccgcgccg ctacccgcgc tgctctacgg caacgcgccg cgcgcgggcg atctgctcgt
     3121 cgtgctcaac ggaaaggtcg cgggcgtctc accgacgttc cgcgacggcg accaagcgca
     3181 ccgcttcgca gtgatggttc ccgaaccatt gttcagaacg ggtgcgaacg atctacagct
     3241 ctactggctg taggaggcgc ttttcaggtg cgattttcgc acctgaaaag cgcctcatcg
     3301 ttctcgttag atgttgccgc tcagtgcggc gaagtgcatg tagccgccgc ggtcggtcga
     3361 cgtgttctgc cacacgtgct tgatctggcg gtactcgttg tagccgtagt cgccgagctc
     3421 gcggccgatg cccgactgct tgtagccacc gaacggctgc gtcggcgtga tcatgtggta
     3481 gtcgttcacc cacaccgtgc cggtgcgcat cgcggcggcg acgccgtgcg cacggtcaag
     3541 gttcttcgac cacacaccac caccgagccc gtaaatcgag tcgttggcga tcttgatcgc
     3601 ctcgtcgtcg gagtcgaacg ggatgacgca gagcaccgga ccgaagattt cttcctgcgc
     3661 gatcttcatc gagttgtcgg cctcgaagat ggtcggcagg tagtaggcac tctggtcgag
     3721 cccggccgcc aggttcgcgg gcttcgcacc gccgcacagc agcttggcgc cttcgtcgag
     3781 cccgagcttc acgtaccgct cggtggtgtc gacttggctc ggcgcgacga gcgggccgag
     3841 gtcggtgttc atgtccatgt tgtcgccgat gacgatcttc ccggcgcgct ccacgagcat
     3901 gccgacaaac tcgtcgtaa
//
LOCUS       DASUGA010000433         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1418722, whole genome shotgun sequence.
ACCESSION   DASUGA010000433 DASUGA010000000
VERSION     DASUGA010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418722"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..366)
                     /locus_tag="VFX21_08565"
     CDS             complement(163..366)
                     /locus_tag="VFX21_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096052.1"
                     /translation="MDRTKPNDETRAFEAEDAQTAHAPDDEPTTDEEAAAERAGDVDP
                     EVAAHYEEMAERGANQKGEGRIP"
     gene            complement(418..1473)
                     /locus_tag="VFX21_08570"
     CDS             complement(418..1473)
                     /locus_tag="VFX21_08570"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEX5096053.1"
                     /translation="MTALLDDDLPPGLFWVDDSAPGIRRRKRGQGFSYTDADGRAVRD
                     EDTLARIRALAVPPAWTDVWICSDALGHLQATGRDAKGRKQYRYNARFRAHRDETKFA
                     RLYEFGSALPDIRRTVADDLGRAGMPIEKVTAAVVRLLEATLVRVGNEEYARENGSYG
                     LTTLRDKHAVVGSGGFRLVFKGKHGISTNVAVQDRRLRSVVKRCQDLPGQVLFQYIDD
                     DGESRPVSSSDVNAYLRRATTLDITAKDFRTWMATLLAACAFAELPPPRSHRQGRQST
                     ARVLEVVADHLGNTPAVCRASYVHPLVIDAYADSSFPDLWSAASARGSRLLIPEERKL
                     CALLRPNRRRATRLRAA"
     gene            1574..>2202
                     /locus_tag="VFX21_08575"
     CDS             1574..>2202
                     /locus_tag="VFX21_08575"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HEX5096054.1"
                     /translation="MRIEDGVRVPDETERIAIERVRPDVDCGRFASKRVLGDIVDVEA
                     DIFTHGHDEIAANVEFRQAGSHSWRSVAMEPLGNDRWRGCFPVDRIGEYRFRVVAWVD
                     EFASWRRDLLAKIGAAQDVSLDREVGARLAEEAAKRAGARDQDALVKWAARLRGGLDP
                     KTIGALIQLVALGRRTADRSRAAVATTTSPVWVDRPLAGCSAWYELFPRS"
BASE COUNT          297 a          751 c          785 g          369 t
ORIGIN      
        1 acagcgtccg cgttcgatgc gtcgatctca ccgcggatgt ggacgaccgg tgtttcgtcc
       61 gtgtcgtcga catgcacttc ggccagcttc acgagctccc cactctcagc agtggcgggg
      121 ctcgattatc gcacgccgcc ggtcacatgg cggccgcgac ggtcacggta tgcggccttc
      181 gcccttctgg ttggcgccgc gttcggccat ctcttcgtag tgcgcggcaa cttcggggtc
      241 gacgtcaccg gcgcgctcgg ccgccgcttc ttcgtcggtc gtcggttcgt cgtcgggtgc
      301 gtgcgccgtt tgggcgtcct cggcctcgaa cgcacgagtc tcatcgttcg gctttgttcg
      361 gtccacggcg tttgctcctt cggtcgtcgt ctgtcactac ccgttgccgc cgcgtgctca
      421 tgccgcccgc agacgcgtcg cgcgccgacg gttcggtcgc aacagcgcgc acagctttcg
      481 ttcttcgggg atgagcagcc ggctgccgcg cgccgatgcc gccgaccaga ggtccgggaa
      541 cgagctgtcg gcgtaggcgt cgatgacaag tggatgcacg tagctcgccc ggcacaccgc
      601 cggcgtgttg ccgaggtggt cggcgacgac ctcgagcacg cgcgcggtcg attggcggcc
      661 ctggcggtgc gagcgcggcg gcgggagctc cgcgaacgcg cacgccgcga ggagtgtcgc
      721 catccacgtg cgaaagtcct tggcggtgat gtcgagcgtt gtcgcgcgcc ggaggtaggc
      781 attcacgtcg gaagacgaga ccgggcgcga ctcgccgtcg tcgtcgatgt actggaacag
      841 cacctgcccg ggaaggtcct ggcaccgctt cacgacgctc cggagccgcc ggtcctgcac
      901 ggcgacgttg gtgctgatcc cgtgcttgcc cttgaagacg agacggaacc ctcccgagcc
      961 gacgacggcg tgcttgtcgc gcaaggtcgt caggccgtag gagccgttct cgcgcgcgta
     1021 ctcctcgttg ccgactcgga cgagcgtcgc ctcgaggagc cgcaccacgg ccgcggtcac
     1081 cttctcgatc ggcatgccgg cgcgtccgag gtcgtcggcg acggtgcgac ggatgtcggg
     1141 gagcgcgctc ccgaactcgt agagacgcgc gaacttcgtc tcgtcacggt gcgcgcggaa
     1201 ccgcgcgttg taccggtact gcttgcggcc tttcgcgtcg cgtcctgtcg cctggaggtg
     1261 accgagcgcg tcagagcaga tccatacatc ggtccacgcc ggcggaactg cgagcgcgcg
     1321 tatgcgcgcc agcgtgtctt cgtcccggac ggcgcgtccg tcggcatcgg tgtaggagaa
     1381 cccctgcccg cgcttgcggc gacggatgcc gggcgcgctg tcgtcgaccc agaagagacc
     1441 gggtggcaga tcgtcgtcga gcagcgccgt catcgttgag cacgtccccc gtgacgcaac
     1501 tgcccaaacc acgccgcgca gcgcgcaacg cggttgcaac agcgcgtacg gcgggtacac
     1561 cgcgcgcgcc cccgtgagga tcgaggacgg cgtgcgggtc cccgacgaaa ccgagcgaat
     1621 tgcgatcgag cgcgtgcgtc ccgacgtgga ctgcggccga ttcgcgagca aacgcgtgct
     1681 cggtgacatc gtcgacgtgg aagccgacat cttcacgcac ggccacgacg agatcgcggc
     1741 gaacgtcgag tttcgccagg ccggctcgca ttcatggcga agcgtcgcga tggagccgct
     1801 cggcaacgac cggtggcgcg ggtgctttcc ggtcgaccgg atcggcgagt accggttccg
     1861 cgtcgtcgcg tgggtcgacg agttcgcgtc gtggcgacgc gacctcctcg cgaagatcgg
     1921 cgctgcgcaa gacgtgtcgc tcgatcgcga ggtcggcgcc cgcctcgccg aggaggcggc
     1981 caagcgcgcc ggtgctcgcg accaggacgc gctcgtgaag tgggccgccc gcctgcgcgg
     2041 cgggctcgac ccgaagacga tcggcgcgct gatccagctg gtcgcgctcg ggcggcgtac
     2101 ggccgaccga tcgcgggccg cggtggccac gacgacatcg ccggtgtggg tcgaccgtcc
     2161 gctcgcgggg tgttcggcgt ggtacgagct gttcccgcgc tc
//
LOCUS       DASUGA010000434         2193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_431848, whole genome shotgun sequence.
ACCESSION   DASUGA010000434 DASUGA010000000
VERSION     DASUGA010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431848"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..155)
                     /locus_tag="VFX21_08580"
     CDS             complement(33..155)
                     /locus_tag="VFX21_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096055.1"
                     /translation="MPRIIRDAATISGTTIATVHTSAWMIACRVCQQMIIAMTT"
     gene            complement(136..1077)
                     /locus_tag="VFX21_08585"
     CDS             complement(136..1077)
                     /locus_tag="VFX21_08585"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5096056.1"
                     /translation="MSVLLVLVLAGVVAAALAAVAFKVVNRDAGSATHGPVHATADAV
                     QTQLVRHRSVARFVRDRLSPASATGLALTVALALLLAVVALAFQVRRQSFVADADLDV
                     AQWAGRHATSTSTEFLRRFTDLGSTPVVIVITAVVAIVELVRTRSRGVIVFLAVTVLG
                     IGLANGLAKAVVGRPRPDVDRLVHASGMSFPSGHSAAAAACFAACALCLGRRRALGTR
                     LVITVVAAAFAVMVATSRVLLGVHWASDAFAGLCFGSAWVALCAIAFGGRLLRFGAPV
                     EIAQRATGEALPDDDADGTEHGADRDVRQEVHAQDHP"
     gene            1165..2049
                     /locus_tag="VFX21_08590"
     CDS             1165..2049
                     /locus_tag="VFX21_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX5096057.1"
                     /translation="MTKRVAVIAHSGKQLGGGLDELRRLLHEHGVEPRWYEVPKSKKA
                     PKCVRRALDDGAELLIVWGGDGMVQRCAHEAAGSGATLAIIPAGTANLLATNLGIPID
                     LAAAVSVALQGHRRKLDLGTINGEHFAVMAGAGFDAAMIRDADSGLKDRIGRAAYVLT
                     GAKNIRGNAVRARIKVDGEKFFKGDVSCILVGNVGALFGGMTVFPEAEPDDGSLELAV
                     VTATNLLEWSRVFMRTARSKPADSPFVRVVPARSVRVRFDRPMPYELDGGDRPPTDRL
                     KIDVEPLAVSVCVPAAAA"
BASE COUNT          365 a          751 c          769 g          308 t
ORIGIN      
        1 ccgtcgggcc atgtggccgc gtcggtcgtg ttctacgtgg tcatcgcgat gatcatctgt
       61 tggcacacgc ggcacgcgat catccatgca ctggtgtgga cggttgcgat cgtggtgccg
      121 ctgatcgtcg ccgcatcacg gatgatcctg ggcatgcact tcctgtctga cgtcacgatc
      181 ggcgccgtgc tcggtgccgt cagcatcgtc atcggggagc gcctcgcccg tcgcacgctg
      241 tgcgatctcg accggcgcac cgaagcgcag caggcgtccg ccgaacgcga tggcgcagag
      301 cgcaacccac gccgacccga aacacagccc cgcgaacgcg tcactcgccc agtgcacgcc
      361 caacagaacc cgtgacgtgg ccaccatcac cgcgaacgcg gccgcaacga ccgtgatgac
      421 gaggcgcgtg ccgagcgcgc gccgccgacc gagacacagc gcgcacgccg cgaaacacgc
      481 cgcggcggcg gccgagtgcc cactcggaaa cgacatgccg gacgcgtgca cgaggcgatc
      541 gacgtcgggg cgcgggcggc cgaccacggc tttcgccagg ccgttggcca gcccgatgcc
      601 gagcaccgtg acggcgagga acacgatcac tcctcgcgac cgtgtgcgga ccagctcgac
      661 gatcgcgacc accgccgtga tgacgatcac caccggcgtg ctaccgaggt ccgtgaaccg
      721 ccgcaggaac tccgtgctcg tgctcgtggc gtgccggccc gcccattgcg cgacgtcgag
      781 gtcggcatcg gccacgaaag actggcgtcg cacctggaac gcgagcgcga cgacggcgag
      841 gagcagcgcg agtgcgaccg tcagtgcgag acccgttgca ctcgccggac tcaatcgatc
      901 acgcacgaag cgggcgacgc tgcggtgtcg cacgagctgc gtctgcactg cgtccgcagt
      961 tgcgtggacc ggaccgtggg tcgcgctgcc ggcgtctcgg ttcacgacct tgaacgcaac
     1021 ggcggcgagc gcggccgcga cgactccggc gaggacgagc acgagtagga ccgacatcgt
     1081 tcccccaatc aggacacggc accgcggttc ttcccgcggc gtcgcgcccg caaccgcagc
     1141 accggcgcgc gaggaggaca tccgatgacg aagcgggtcg cagtcatcgc acactcggga
     1201 aagcaactcg gtggagggct cgacgagttg cgacgactcc tgcacgagca cggcgtcgag
     1261 ccgcgttggt acgaagtgcc caagagcaag aaggcaccga agtgcgttcg ccgcgccttg
     1321 gacgacgggg cggagctgtt gatcgtgtgg ggcggcgacg ggatggtgca gcgttgcgcg
     1381 cacgaagccg ccgggagtgg tgcgacgctc gcgatcattc ctgcggggac cgcgaacctg
     1441 ctggccacga acctcggaat acccatcgat ctggctgccg cggtctcggt tgcgctccag
     1501 ggacatcgcc gcaagctgga cctgggcacg atcaacggcg agcacttcgc ggtgatggca
     1561 ggagccggtt tcgacgccgc aatgattcgc gacgccgact ccggcctgaa ggaccgcatc
     1621 gggcgcgcgg cgtacgtgtt gaccggcgcc aagaacatcc gaggcaacgc ggtgcgcgcg
     1681 cggatcaagg tggacggcga gaagttcttc aaaggtgacg tgtcctgcat cctggtcggc
     1741 aacgtcggcg cgctgttcgg cggcatgacc gtcttccccg aggcggagcc cgacgacggg
     1801 tcactcgagc tcgccgtggt cacggccacg aacctgctcg agtggtcgcg cgtcttcatg
     1861 cgcaccgccc gctcgaagcc ggccgactcg ccgttcgtgc gcgtggtgcc ggctcgatcg
     1921 gtgcgggtgc ggttcgaccg gccgatgccg tacgagctcg acggcggtga ccgcccgccg
     1981 acggatcggc tgaagatcga cgtcgagccg ctggcggtgt ccgtgtgcgt cccggcagcg
     2041 gccgcatgag taccgcggtc ccgattcccg agacttggga gctcgacggc gacgatgcgc
     2101 gggagacact cctcagcacc ggacgccggc agctcgtgcg tgacgcgttc gtgcgcatgc
     2161 gggcggccga cggcttcagc cacgcccggt cga
//
LOCUS       DASUGA010000435         2742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_197163, whole genome shotgun sequence.
ACCESSION   DASUGA010000435 DASUGA010000000
VERSION     DASUGA010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197163"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VFX21_08595"
     CDS             complement(<1..664)
                     /locus_tag="VFX21_08595"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018837677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX5096058.1"
                     /translation="MTSPEPFTPELLPNIAWEPSSWQRLAAAQQPDWPDVVALDEVLA
                     ELRRMPPLVFAGEARTLKQSLARVAEGEGFLLHAGDCAESFADFSADNIRDKLKVILQ
                     MSVALTYSTGVPVLKIGRIAGQFAKPRSAPTESRDGIELPSFRGDMVNDLAFDAAARR
                     ADPSRLLRVYHQSAATLNLLRAFTKGGFADLNRVHQWNLEFVESSGAGRRYETLANEI
                     DRA"
     gene            complement(686..1120)
                     /locus_tag="VFX21_08600"
     CDS             complement(686..1120)
                     /locus_tag="VFX21_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061136 - regulation of proteasomal protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome activator"
                     /protein_id="HEX5096059.1"
                     /translation="MVDGALAETEEGGEQVARPTKLIRIASMVRSLLEEARRAPLDDA
                     GRRSLRTIHERSIKELEEILSPDLQAELEQVTLPFTTETPSESELRIAQAQLVGWLEG
                     LFHGIQATLFTQQMQAQRQFEDMRQRRGLELPAPDARPGSYL"
     gene            complement(1206..2594)
                     /locus_tag="VFX21_08605"
     CDS             complement(1206..2594)
                     /locus_tag="VFX21_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020517455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5096060.1"
                     /translation="MVDTIPVRSPYDGHLISSVPASTPADVDRAVGVAAGALAKGPLP
                     QWQRAAVLDRAAAALASRTEEYARTIAEEAAKPIKTARVEAQRAISTFQFAAVAARTL
                     AGEMVPMDASAAGANKLGFTLRLPIGVLGAISPFNFPLNLVAHKIAPAIAAGCPVVLK
                     PASQTPLSALALAQLLVEECELPAEQLQVVCGSGGTVGNAIVDHDGIAMISFTGSPEV
                     GWDIRARAARKKVGLELGNNAPLIIEPSADIATAAQKVSVAGFSHAGQSCISTQRIFV
                     HEAVRDAFVDALVPLVEGLVVGDPLDERTDVSALISTGERDRVSDWVEEAVTAGAEVL
                     AGGKVREDGLLMPTVLGGVTPDMKVCALEVFGPVVGLQTYSTLEDALKMANATRYGLQ
                     AAIFTADLDDAMRAAYALDFGGVLVNEVPTYRADQQPYGGVRDSGNTREGPLWAVREM
                     TEERVVIISLPG"
BASE COUNT          416 a          959 c          941 g          426 t
ORIGIN      
        1 tcgcgcggtc gatctcgttg gcgagcgtct cgtagcgccg gccggcaccg ctcgactcga
       61 cgaactcgag gttccactgg tgcacgcggt tgaggtcggc gaaaccgccc ttcgtgaacg
      121 cgcgcagcag gttcaacgtc gccgctgact ggtggtacac gcgcaacaat ctcgacggat
      181 cggcgcgccg ggccgcggcg tcgaacgcga gatcgttcac catgtcgcca cggaacgacg
      241 gcagctcgat gccgtcgcgc gactcggtcg gcgccgaacg cggcttcgcg aactggcccg
      301 cgatacgacc gatcttcagc accggcacac ccgtgctgta ggtcagcgcg accgacatct
      361 ggaggatgac cttcaacttg tcgcggatgt tgtcggcgga gaagtcggcg aaggactccg
      421 cgcaatcgcc cgcgtgcaag aggaagcctt cgccttcggc gacacgcgcc agcgactgct
      481 tcaaggtgcg cgcctcgccc gcgaacacga gcggcggcat acggcgcagc tcggcgagca
      541 cctcgtcgag cgcgaccaca tcgggccagt cgggctgctg cgcagcggcc aggcgctgcc
      601 acgacgacgg ctcccacgcg atgttcggca acaactcggg tgtgaacggt tcgggcgatg
      661 tcattgcgat cgactcacat caaactcaga ggtagctacc ggggcgcgcg tcgggtgccg
      721 gcaactcgag cccccgccgc tgacgcatgt cttcgaactg gcgctgcgcc tgcatctgct
      781 gcgtgaacag cgtcgcttgg atgccgtgga acagtccttc gagccagccg accagttgcg
      841 cctgcgcgat gcgcagctcc gactcgctcg gcgtctcggt ggtgaagggg agcgtcacct
      901 gctcgagctc cgcctggagg tcgggcgaca ggatctcctc gagctccttg atggagcgct
      961 cgtgaatcgt gcgcagcgag cgtcggccgg cgtcgtcgag cggtgcccgg cgcgcttcct
     1021 cgagcagcga ccgcaccatc gacgcgatac ggatcagctt ggtcggccgc gcgacctgct
     1081 caccgccctc ttccgtctcc gcgagggcgc cgtcgaccac ctcgggcgtg aggggttgcc
     1141 cggccgccgg attctgggtc ggttcgaacg aagtcacagc gtcccaggct acctgcccac
     1201 cctgttcagc ccggcaggga aatgatgacg acccgctcct cggtcatctc ccgcaccgcc
     1261 cacagtggcc cctcgcgtgt gttgccgctg tcgcgcacac cgccgtacgg ctgctggtcg
     1321 gcgcgatagg tcggcacctc gttgacgagc acgccaccga aatcgagcgc gtacgcagcc
     1381 cgcatcgcgt cgtcgaggtc ggcggtgaag atcgcggcct ggagcccgta gcgggtggcg
     1441 ttcgccatct tcagcgcgtc ttcgagcgtg ctgtaggtct gcagcccgac gaccgggccg
     1501 aagacctcca gcgcgcacac cttcatgtcg ggcgtgaccc cgccgagcac ggtcggcatc
     1561 aagaggccgt cttcgcggac cttgccgccc gcgagcacct cggcacccgc ggtgacggcc
     1621 tcctcgaccc aatcggacac acggtcgcgt tcaccggtcg agatcagtgc cgacacgtcg
     1681 gtgcgctcgt cgagcggatc gccgacgacg aggccttcga cgagcgggac gagcgcgtcg
     1741 acgaacgcgt cgcgcactgc ttcgtgcacg aagatgcgtt gggtcgagat gcacgactgt
     1801 cccgcgtggc tgaagccggc gaccgagacc ttctgggcgg cggttgcgat gtcggcggac
     1861 ggttcgatga tgagcggcgc gttgttgccc aactcgagcc cgaccttctt gcgggccgcg
     1921 cgggcacgga tgtcccagcc gacctcgggt gagccggtga agctgatcat cgcgatgccg
     1981 tcgtgatcca cgatcgcgtt cccgacggtg ccgccgctcc cgcagacgac ttggagttgc
     2041 tcggcaggca gctcgcactc ctcgacgagc agctgcgcga gcgccagcgc cgacaacggt
     2101 gtctggctcg cgggcttcaa cacgaccggg cagccggccg cgatcgcggg tgcgatcttg
     2161 tgggcgacga ggttcaacgg gaagttgaac ggactgatcg cgccgagcac gccgatcggg
     2221 agccgcagcg tgaacccgag cttgttcgcg ccggccgcac tcgcgtccat cggcaccatc
     2281 tcgcccgcca gggtgcgcgc cgcgaccgcc gcgaactgga acgtcgagat cgcgcgctgc
     2341 gcctcgacgc gcgccgtctt gatcggcttc gccgcttcct ccgcgatggt gcgcgcgtac
     2401 tcctcggtgc gcgacgcgag cgcggctgcg gcgcggtcga gcaccgcggc ccgctgccac
     2461 tgcggcaacg ggcctttcgc caacgcaccc gcggcgacgc cgacggcgcg gtcgacgtcg
     2521 gccggcgtcg acgcgggcac cgacgagatc aagtgcccgt cgtagggaga gcgaactggg
     2581 atcgtgtcaa ccacgacccc gaggctacga cgcgacgccg gctgccttct tgcgcggtgc
     2641 cgggctgtcg agcgtctcga ggtccgcgag gcgcgggtcg ttcgagaaca cgtcgatgat
     2701 cggcatgcgc agcgcgagcc ggcgttgcag gcgcgccacc tc
//
LOCUS       DASUGA010000436         3533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_419519, whole genome shotgun sequence.
ACCESSION   DASUGA010000436 DASUGA010000000
VERSION     DASUGA010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419519"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VFX21_08610"
     CDS             <1..541
                     /locus_tag="VFX21_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5096061.1"
                     /translation="GAAIETQVADAEALPFEDATFDATLSTFGVMFTPDQERAAAEMA
                     RVTRPGGKIGLANWTPGGFVGAMLRTVGGHVPPPAGLHSPAEWGTDTRLAELFAGSAS
                     VAATAKHFVFRAPSIDAWLELFASYYGPIVAALASLDDDRRSGLRRDLYSLAAAHNEA
                     TDGTARISGEYLEVVATVR"
     gene            complement(534..1355)
                     /locus_tag="VFX21_08615"
     CDS             complement(534..1355)
                     /locus_tag="VFX21_08615"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX5096062.1"
                     /translation="MPPQPGVYFLLAENTELLYVGKASNLRTRLAQHARAAGATTDVR
                     LRALYARTGEVRWEAAPDEAAAAAREADVIVALQPRFNASIVDEGRWNYVTIEPGRHG
                     PASMRLGLTPSTDRPAQAYGCFPHLGHGLSSGPGIACSDGYTALLRLLWVCSTGGTLP
                     IPSRLSTGRVCDAIEIPVPAAWRAPLHALLSGTSCRLLADLAVVETAHAPHLRSGLLR
                     DAVAAESFFRHGPVAVRRLRLRHGRRGRTITRDQVRAMVTADLHEAIGPFRLPVT"
     gene            complement(1438..1890)
                     /locus_tag="VFX21_08620"
     CDS             complement(1438..1890)
                     /locus_tag="VFX21_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007446122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096063.1"
                     /translation="MATTVAPTARPAPALSTSYRLRTDPTYQAFWILRAGFVVAPILF
                     GLDKFAHVLVDWDKYLAPEFTDLFNAQAHTLMYAVGAIEIVAGLIVLARPQIGAYIVA
                     AWLAGIIVNLLMLGDFYDVALRDFGLFLAAIALGRLAAAEAPSHRVLS"
     gene            1980..2684
                     /locus_tag="VFX21_08625"
     CDS             1980..2684
                     /locus_tag="VFX21_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003996717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5096064.1"
                     /translation="MTRDMSLDAVSALAEPTRRSLYDYIANTGGWVSRDQAADALDLE
                     RGNAAHHLDRLASEGLLDVEYRRLSGKQGPGAGRPAKLYRRAQKEFDVALPPRDYALA
                     GQLLAAAVDQARGDGTDLDTALDEAASDEGHRIGADINARLRAGAARKKDLRRRVVLD
                     ALADHGYEPYTRDNGTVVLRNCPFHQLAQQHTNLICGLNLCVLSTAIDDVGDTGLVAH
                     LEPEDDHCCVKLKPPG"
     gene            2722..2919
                     /locus_tag="VFX21_08630"
     CDS             2722..2919
                     /locus_tag="VFX21_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096065.1"
                     /translation="MILTVVLNVALRLFPGARDRLSESLDRVAGDGEPNPARVIVPWK
                     AMLIGSLLLTVAINVVPVIFR"
     gene            complement(2906..>3533)
                     /locus_tag="VFX21_08635"
     CDS             complement(2906..>3533)
                     /locus_tag="VFX21_08635"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5096066.1"
                     /translation="KYAQQRIQAVRDDPPSDVMRAIVDGMATGALSEDEGAATFVLLL
                     IAGHETSSNLIALSVYTLLQNPEVLAAVRADANLVAPAIEESLRFATTVPQMARYTKE
                     DVELDGVEIAKDEVLIVRLDLANRDPEMFADPDTFSLTRPAKPTHLSFGAGIHFCLGA
                     VLARLEAEVALNFLLPRIAPEEHGDRITWRTTFSVRGPETFPLQLTGR"
BASE COUNT          569 a         1214 c         1231 g          519 t
ORIGIN      
        1 cggcgcggcc atcgagacgc aggtcgccga cgccgaggcg ctgccgttcg aggacgccac
       61 gttcgacgcg acactctcga cgttcggcgt gatgttcaca cccgaccagg aacgcgcggc
      121 cgccgagatg gcacgcgtca cccgtcccgg cggcaagatc ggcctcgcga actggacacc
      181 gggcggcttc gtcggcgcga tgctgcgcac ggtcggaggc cacgtgccgc cgcccgccgg
      241 tctgcactcg cccgccgaat ggggcaccga cacgcgcctc gccgagttgt tcgccggctc
      301 cgcttccgtc gcggcgaccg cgaagcactt cgtgttccgc gccccgtcga tcgacgcgtg
      361 gctcgagctg ttcgcgtcgt actatggacc aatcgtcgcc gcgctggcgt cactcgacga
      421 cgaccgccgg agcgggctgc gccgcgactt gtactcgttg gccgcggcac acaacgaggc
      481 caccgacggc accgcgcgca tctccgggga gtacctggaa gtcgtcgcga ccgtcaggtg
      541 accgggaggc ggaacggtcc gatggcctcg tgcaggtcgg ccgtcaccat cgctcgtacc
      601 tgatcgcgag tgatggtgcg gccgcgccgg ccgtgccgga gccggaggcg ccgaaccgcg
      661 acgggaccgt ggcgaaagaa cgactccgcg gcgacggcgt cgcgcaggag accggaccgg
      721 agatgcggcg catgcgcggt ctcgacgacg gcgagatccg caagcaggcg acaactcgtg
      781 cccgagagca acgcgtgcaa cggcgcgcgc cacgccgcag gcaccggtat ctcgatggcg
      841 tcacacactc gccccgtcga caggcggctc gggatcggga gcgttccccc ggtcgagcac
      901 acccacaaca accgaagcaa cgcggtgtac ccgtcgctgc acgcgatccc cggccccgag
      961 ctcaggccgt gacccagatg cggaaagcag ccgtacgcct gcgccggccg gtccgtcgac
     1021 ggtgtcaggc cgagacgcat cgacgccggg ccgtgacggc ccggctcgat cgtcacgtag
     1081 ttccagcgcc cttcgtcgac aatcgacgcg ttgaagcgcg gctgcaacgc gacgatgaca
     1141 tcggcctcgc gcgccgccgc ggccgcctcg tcgggcgcgg cctcccatcg cacttctccc
     1201 gtccgggcgt acaacgctct cagccggacg tcggtcgtcg cgcccgcagc tcgcgcatgt
     1261 tgcgcgagcc gcgtgcggag attcgacgcc ttgccgacat agagcagttc cgtgttctcc
     1321 gcgaggagga agtacacacc gggctgaggc ggcacgtcac cacgtcgaag tgcatgcgcg
     1381 ggagaggaga cgtcggccat cgctgcgcgc atgttcgcgc gtcgaggccg tcgatgctca
     1441 cgacagcacg cggtgtgacg gtgcctccgc ggccgcgagg cggccgaggg cgatggcggc
     1501 gaggaacagg ccgaagtcgc gcagcgcgac gtcgtagaaa tcgccgagca tcagcaggtt
     1561 gacgatgatg cccgcgagcc acgcggccac gatgtacgcg ccgatctgcg ggcgggcgag
     1621 cacgatgagc ccggcgacga tctcgatcgc gcccaccgcg tacatgaggg tgtgggcctg
     1681 ggcgttgaag aggtcggtga actcgggtgc caggtacttg tcccagtcga cgaggacgtg
     1741 ggcgaacttg tcgaggccga acaggatcgg cgccacgacg aagccggccc gcaggatcca
     1801 gaacgcctgg taggtggggt cggtccggag ccggtacgag gtcgagagcg cgggcgcggg
     1861 gcgagcggtg ggggcgacgg tggtggccat gtgagcggct tcccttctaa cgacaatctg
     1921 tccttgtctt agtgaacggg aagctcgcct ctatgtcaag tatcgattag tcttagagca
     1981 tgacccggga tatgtccctc gacgcggtgt ccgcgctggc cgagcccacg cgccggtcgc
     2041 tctacgacta catcgcgaac acgggcggct gggtcagtcg cgaccaagcc gccgacgcgc
     2101 tcgatctcga acgcggcaac gccgcccatc acctcgaccg gctggcgagc gaaggactgc
     2161 tcgacgtcga gtaccggcga ctgtcgggga agcagggccc cggcgcgggt cgccccgcca
     2221 agctctaccg gcgcgcgcag aaggagttcg acgtcgcgct gcccccgcgc gactacgcgc
     2281 tcgcgggaca actgctcgcc gccgccgtcg accaggcgcg tggcgacggg accgacctcg
     2341 acaccgcact cgacgaagcg gccagcgacg aaggccaccg catcggcgcc gacatcaatg
     2401 cccggctgcg cgccggtgcg gcgcgcaaga aggatctccg ccgccgcgtc gtgctcgacg
     2461 cactcgccga ccacggctac gagccgtaca cgcgcgacaa cggcactgtc gtgttgcgca
     2521 actgcccctt ccaccaactc gcgcagcagc acaccaatct gatctgcggg ctcaacctct
     2581 gcgtgttgag caccgcgatc gacgacgtcg gcgacaccgg actcgttgcg cacctggagc
     2641 ccgaggacga ccactgctgc gtcaagctca agcccccggg ctgactcgat gtcgtggttg
     2701 gcgttttcgc tcgtcgcttc ggtgatcttg acggttgtgc tgaacgtcgc cctgcggctg
     2761 ttcccaggcg cccgcgaccg actcagcgag tcgctcgacc gcgtcgcggg cgacggcgag
     2821 cccaatccgg cacgagtgat cgtcccctgg aaggcgatgc tgatcggatc gttgctcttg
     2881 acggtcgcga tcaacgtcgt gccggtcatc ttccggtgag ctgcagcggg aaggtctcgg
     2941 ggccgcggac ggagaacgtc gtgcgccagg tgatgcggtc gccgtgctct tcgggcgcga
     3001 tgcgcgggag caagaagttg agcgcgacct cggcttcgag ccgtgcgagc acggcgccca
     3061 ggcagaagtg aatgcccgca ccgaatgaga gatgcgtcgg tttggccgga cgcgtgagcg
     3121 agaacgtgtc ggggtccgcg aacatctcgg ggtcgcggtt ggcgaggtcg agccgcacga
     3181 tcagcacttc gtccttggcg atctcgacgc cgtcgagctc gacgtcttcc ttcgtatagc
     3241 gagccatctg cggcaccgtc gttgcgaagc gcagcgattc ctcgatcgcg ggcgccacga
     3301 ggttcgcgtc cgcgcgcacg gcggccagca cctccgggtt ctgaagcagc gtgtagaccg
     3361 agagtgcgat caaattcgag ctggtctcgt gcccggcgat cagcagcagc acgaacgttg
     3421 ccgcgccctc gtcttcgctc agcgcgccgg tggccatgcc gtcgacgatg gcgcgcatga
     3481 catcgctggg cgggtcgtcg cgcacggcct gaatgcgctg ctgcgcgtac ttg
//
LOCUS       DASUGA010000437         2798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_36708, whole genome shotgun sequence.
ACCESSION   DASUGA010000437 DASUGA010000000
VERSION     DASUGA010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36708"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="VFX21_08640"
     CDS             complement(<1..987)
                     /locus_tag="VFX21_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5096067.1"
                     /translation="MSIEELMGREDANDLDVIVDLAIDNPHVGEVVHTVADNADAIFT
                     WDYEKGKRTALNKLYEKAKSSQWNGETDLPWDTDVDQERVTIEALGAEPNWEFAGLDV
                     AGTPLEKWGSKEWIRFNIEQQNWMLSQFMHGEQGALLCTAKIVETVPWIDAKYYAATQ
                     VMDEARHVEVFAKYLDTKLSGHYPLNTHLGMLLDDIIVDSRWDMTYLGMQIMVEGLAL
                     AAFGYMHMLTPEPLLKQLLRYVMSDEARHVAFGVLSLKEYYEALSDAELKERQEFAFE
                     AALRMRDRLLAVEVWERIDVDPKFIISHLRGDQLEDPFQQLLFSKIVPNCKKL"
     gene            1080..1724
                     /locus_tag="VFX21_08645"
     CDS             1080..1724
                     /locus_tag="VFX21_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5096068.1"
                     /translation="MSRLRWGDEAPTTTSAARDRLVDAAEVCVDRFGLAKTTLEDVAR
                     QAEVSRATVYRYFANRDELMLEVLLRDLERSRHRDLVEFFDDATDPASFGRSLVDAAA
                     YLLETIRSSPKLQLLLHREGSNFNSTIAGASEALFRQWIDDVAPYLSEAQRAGLIRAD
                     LDAAEAAEWILRVILSLLTIEGPRHHSPEEERRLMTTFVIPSLLPAAVLEVATD"
     gene            complement(1721..>2798)
                     /locus_tag="VFX21_08650"
     CDS             complement(1721..>2798)
                     /locus_tag="VFX21_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013425923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX5096069.1"
                     /translation="DYRYCWLRDATFTLTALSDAGYSDEAMAWSDWLRRAVAGSPKKM
                     QIMYGVGGERRLVETELKWLTGYEHSTPVRVGNAASEQFQLDVYGEVMDMFLHSRHCL
                     GHELPDDVWDLAGILVEHVASVWTQPDDGIWEVRGPRRHFVHSKVMAWVAVDRWVQLC
                     EAGRTDEPLDRWYDLRDDMHREICEKGYNASVEAFTQYYGSTELDASCLMMALVGFLP
                     PDDPRIVNTVEAIERELLVDGFVKRYHTSAQHAADDGATTTTVDGLPPGEGAFLLTTF
                     WLLDNLVLMGRVDDARRIFDRLAGLANDLGLLSEEYDVGRSRMVGNFPQAFSHVGLIV
                     SAMNLSGMPEIPAAHRCTPTPARS"
BASE COUNT          452 a          928 c          915 g          503 t
ORIGIN      
        1 gagcttcttg cagttgggca cgatcttgga gaacagcagc tgctggaatg ggtcctcgag
       61 ctggtcgccg cgcaggtggc tgatgatgaa cttgggatcg acgtcgatgc gctcccacac
      121 ctcaacggcc aacagacggt cgcgcatgcg cagggcggcc tcgaacgcga actcctgacg
      181 ctccttgagc tctgcgtcgg agagggcctc gtagtactcc ttgagcgaga gcacgccgaa
      241 cgcgacgtgg cgcgcttcgt cgctcatcac gtagcgcagc aactgcttga gcagcggctc
      301 gggcgtgagc atgtgcatgt agccgaacgc ggcaagggcg agcccctcga ccatgatctg
      361 catgccgagg taggtcatgt cccaccggct gtcgacgatg atgtcgtcga gcagcatccc
      421 gaggtgcgtg ttgagtgggt agtgacccga cagcttggtg tcgaggtact tggcgaacac
      481 ctccacgtgg cgggcctcgt ccatcacctg cgtggcggcg tagtacttgg cgtcgatcca
      541 cggcacggtc tcgacgatct tggccgtgca cagcagcgcg ccttgctcgc cgtgcatgaa
      601 ctggctgagc atccagttct gctgctcgat gttgaagcgg atccactcct tcgaacccca
      661 cttctcgagc ggggtgccgg cgacgtcgag cccggcgaac tcccagttgg gttcggcgcc
      721 gagcgcctcg atcgtgacgc gctcctggtc gacgtcggtg tcccacggca gatcggtctc
      781 gccgttccac tgcgagctct tggccttctc gtagagcttg ttgagcgcgg tgcgcttccc
      841 cttctcgtag tcccacgtga agatggcgtc ggcgttgtcg gccacggtgt gcaccacctc
      901 gccgacgtgg gggttgtcga tcgcgaggtc gacgatcaca tcgagatcgt tggcgtcctc
      961 ccggcccatg agctcttcga tggacatgac gttccccctc gtcgcgatgg actcttgcgc
     1021 gaatgataca aagtgtgtcg tttgtcgcaa gtgacgtcgg tcgcacatac actcagctcg
     1081 tgagcaggct caggtggggt gacgaggcgc cgaccaccac gagcgcggcc cgcgatcgcc
     1141 tggtggacgc ggccgaggtg tgcgtcgacc gtttcgggct ggccaagacc accctcgagg
     1201 acgtggcccg ccaggccgag gtgtcgcgag ccaccgtgta ccggtacttc gccaaccgtg
     1261 acgagctcat gctcgaggtg ctcctgcgcg atctcgagcg ctcacggcat cgcgacctgg
     1321 tcgagttctt cgacgacgcc accgatcccg cctcgttcgg ccgctcgctc gtggacgcgg
     1381 ccgcctacct gctcgagacc attcgcagca gccccaagct gcagctcctg ttgcaccgcg
     1441 aaggctccaa cttcaactcc acgatcgcgg gcgcgtccga ggcgttgttc cgccagtgga
     1501 tcgacgacgt cgctccctac ctcagcgagg cgcagcgcgc cgggctcata cgggccgacc
     1561 tcgatgccgc agaagcggcg gaatggatcc tgcgcgtgat cctctcgctg ctcaccatcg
     1621 agggaccgcg gcaccactca ccggaggagg agcggcgact gatgacgacg ttcgtgatcc
     1681 cgtcgttgct ccccgccgcc gtgctcgagg tggcgactga ctaggagcga gcaggggtgg
     1741 gcgtgcaccg gtgcgcggcg gggatctccg gcatgccgct caggttcatc gcgctgacga
     1801 tcaagccgac gtgcgagaac gcctgtggga agttgcccac catccggctg cgaccgacgt
     1861 cgtactcctc ggacagcaac ccgagatcgt tggccagccc ggcgagtcga tcgaagatcc
     1921 ttcgcgcgtc gtcgacccgg cccatgagca cgaggttgtc gagcagccag aacgtggtga
     1981 gcagaaacgc gccctcgccc ggcggaagac cgtcgacggt tgtggtcgtg gccccgtcgt
     2041 cggcggcgtg ctgggccgag gtgtgatagc gcttcacgaa cccgtccacg agcaactcgc
     2101 gctcgatcgc ctcgacggtg ttgacgatgc ggggatcgtc gggcgggagg aagccgacca
     2161 gcgccatcat cagacagctc gcatcgagct cggtggaccc gtagtactgc gtgaacgcct
     2221 cgaccgacgc gttgtaaccc ttctcgcaga tctcgcggtg catgtcgtcg cggaggtcgt
     2281 accaacggtc gagtggctcg tcggtacggc cggcttcgca gagctgcacc caccggtcga
     2341 ccgcgaccca cgccatcacc ttggagtgga cgaagtgccg gcgcggtccg cgcacctccc
     2401 agatcccgtc gtcgggttgg gtccagaccg acgcgacgtg ctccacgagg atgccggcca
     2461 ggtcccacac gtcgtcgggt agctcgtgac caaggcagtg tcgcgaatgg aggaacatgt
     2521 ccatgacctc gccgtacaca tcgagctgga actgctcgct ggccgcgttg ccgacccgta
     2581 cgggagtcga gtgctcgtac ccggtgagcc acttcagctc ggtctcgaca agacgacgct
     2641 ccccgcccac gccgtacatg atctgcatct tcttcgggct gccggcgacg gcccgacgga
     2701 gccagtcgct ccacgccatc gcctcgtcgc tgtagccggc atcgctcagc gcggtgagcg
     2761 tgaacgtcgc gtcgcgcagc cagcagtatc ggtagtcc
//
LOCUS       DASUGA010000438         5004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_405818, whole genome shotgun sequence.
ACCESSION   DASUGA010000438 DASUGA010000000
VERSION     DASUGA010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405818"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="VFX21_08655"
     CDS             complement(<1..1035)
                     /locus_tag="VFX21_08655"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5096070.1"
                     /translation="MTDEPGTMRAWRIHEYGRPQDVLRLDSVPIPEPDDGEVRVRVQA
                     IPLNLNDLERITGGNMMVRPELPYSPGMEVMGVVDACGNGTSEWQGRRVVAMTRQAHG
                     GFAEYAICPVVSAFAMPDAIPLPDAAALYFPFHLAWIGLVDRATLRPGESVLIHAAAG
                     GSGSAAVQLAKHLGARVFATAGTDAKVELCRSLGADVAINYNDQDFAAIVLEETANRG
                     VDVVFDNVGEAVMEASMKCTAYNGRYLMMGFASNKEVADEPFIVPRRVALGNLKLCGV
                     LLAYAAPDMAEMLKTAMGWNFASAELGTRVMRDIVDLVVAGKLRAVVGSVVDFGGVPA
                     AIEALAGRETT"
     gene            1084..1590
                     /locus_tag="VFX21_08660"
     CDS             1084..1590
                     /locus_tag="VFX21_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014158028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEX5096071.1"
                     /translation="MDDTAVLIDGFGRVHEEVHAAVDGLSPEQLTVRLDAAANTIAWL
                     AWHLTRIQDDHIADVAGHEDVWLADGWRERFALPFEPHATGYGHTSEDVGAVRADAAL
                     LLAYFDAVHERTIGYLRTIEPNEMERIVDDRWDPPVTLAVRLVSVLSDDLQHAGQAAY
                     VRGIIERR"
     gene            1613..2767
                     /locus_tag="VFX21_08665"
     CDS             1613..2767
                     /locus_tag="VFX21_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096072.1"
                     /translation="MKRVLVAVCALLALIGLSACDVPLKTAASTAKIELLATSTNGTW
                     QYDYYRNNAYPCSISGYQTFVVGRKVGSSSDARPLWVFMHGGGAGYFDENGNPIPGRG
                     QKVEEDFDSLRGRLTNGGLSKNIRSDAAGFRMLAVSMCSHDVYAGMNTSDPHNPNTTP
                     DGKPRPTTGLISTKSAIQFTQGTYPTTKTFLAGGSAGSVGTFNVAWSMQLQGIAPAGI
                     VADASLINVESRQAAFQQGVCTEDNDPARIAGISGRVHPDLADINNEVDKIVANGKLT
                     VPIMHIWNHGDTNTCGATPVTCPLRDGTTVTMGLTDCMHRPLQIVISAQGPTSKSRNL
                     PVCVDADDVKGDCSTHVVTTRASVLNTDPASPADYIGAVVDWVHARLADA"
     gene            complement(2869..3249)
                     /locus_tag="VFX21_08670"
     CDS             complement(2869..3249)
                     /locus_tag="VFX21_08670"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX5096073.1"
                     /translation="MANDEKPHVYVPPGEAPPGELTIEDLEPGSGRAAAAGDEVTVHY
                     VGHSWSTRKQFDASWDRGETFSFRLGAGDVIAGWDQGVNGMQVGGRRRLVIPPNLGYG
                     KRGAGGVIGPNETLVFVVDLISVR"
     gene            complement(3268..4095)
                     /locus_tag="VFX21_08675"
     CDS             complement(3268..4095)
                     /locus_tag="VFX21_08675"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HEX5096074.1"
                     /translation="MDEEVWDLEMKIAAQADLLETVSARPTGTDRAVGDVPDWFGPVA
                     FGGFILGQTLFAATTTVDADDGRRPHSLHCYFLRPVLAGTPVEYEIVRVRDGRSYATR
                     RVDAFQSGKHACTMMVSFAADTDGYEYELPVLDTLPDLDGLEEWQGQGPWLERVAGPT
                     EPAADGTRQSTHRAYFKLPAPMSDDPMLHAAIVAWISDMTFTGARPHNLDPVMEGIVS
                     LDHALWFHRPLRVDDWVYYDLHSLVNAGGRGTVRGTLHTADGRLCASVGQELLLRVI"
     gene            4157..4654
                     /locus_tag="VFX21_08680"
     CDS             4157..4654
                     /locus_tag="VFX21_08680"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX5096075.1"
                     /translation="MKITRGSLVRRVARQMLAGMFINGGLDALRNPAPKVTRAERVVE
                     PLNDALGIQTDAETLIRANGAVMVAGGVLLSTGRLPRLASLVLATTLIPTTFAGHAFW
                     AEEDPQTRAQQRTQFFKNASMFGGLLFAALDTGGRPSIPWRAKRAAHKAAEQVSEHVT
                     LPHAA"
     gene            4673..>5004
                     /locus_tag="VFX21_08685"
     CDS             4673..>5004
                     /locus_tag="VFX21_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5096076.1"
                     /translation="MTACISADSHVTEPPGTYVDRIDARFKDRAPRMHFDETLGDVML
                     IDNGATLVPFWLVAAAGRPTEEVRLDTGKRFDELHRGGWDPHARLVDQDTDGVQAEVI
                     YPSVGMIL"
BASE COUNT          856 a         1708 c         1676 g          764 t
ORIGIN      
        1 ggtcgtctcg cgaccggcca gcgcctcgat cgccgcgggc acgccgccga agtcgacgac
       61 gctccccacg acggcccgca acttgcccgc gacgacgagg tcgacgatgt cgcgcatgac
      121 gcgtgtgccg agctcggcgg atgcgaagtt ccaacccatc gcggtcttga gcatctccgc
      181 catgtccggt gcggcatagg cgaggagcac gccgcacagc ttcaggttcc cgagggcaac
      241 ccggcggggg acgatgaacg gctcgtcggc gacttccttg ttcgacgcga agcccatcat
      301 cagatagcgg ccgttgtacg cggtgcattt catcgacgct tccatcacgg cttcgccgac
      361 gttgtcgaaa acgacgtcga caccgcggtt ggcggtctcc tcgaggacga tcgcggcgaa
      421 gtcctggtcg ttgtagttga tcgcgacgtc ggcaccgagc gaacgacaca actcgacctt
      481 ggcatcggtg cccgcggttg cgaacacacg cgcgccgaga tgcttcgcga gttgtacggc
      541 ggccgagccc gacccgccgg ccgcggcgtg gatcaggaca ctctcgccgg ggcggagcgt
      601 ggcacggtcg acgagaccga tccacgcgag gtggaacgga aagtagagcg cggccgcgtc
      661 gggcagcggg atcgcgtccg gcatcgcgaa cgccgagacg accgggcaga tggcgtactc
      721 cgcgaacccg ccgtgcgcct gacgcgtcat cgcgacgacg cgccgcccct gccattcact
      781 tgtcccgttg ccgcacgcgt cgacgacacc catgacttcc atgccggggc tgtacggcag
      841 ctccgggcgc accatcatgt tgccgcccgt gatccgttcg agatcgttga ggttcagcgg
      901 tatcgcctgg acccgcacgc gcacctcacc gtcgtccggc tccgggatcg gcaccgaatc
      961 gaggcgaagg acgtcttgcg ggcggccgta ctcgtggatg cgccacgctc gcatcgtgcc
     1021 gggctcatcg gtcatgaagg caatggtggc cgggacgcct gcgcacccgt caaacttggc
     1081 ggcatggacg acacggcggt actcatcgac ggcttcgggc gggtgcacga agaagtgcac
     1141 gcagcggtcg acgggctgtc gcccgagcaa ctcaccgtgc gactcgacgc ggccgcgaac
     1201 acgatcgcgt ggctggcgtg gcatctcacg cgcatccagg acgaccacat cgcggacgtc
     1261 gccgggcacg aggacgtgtg gttggccgac ggatggcgcg agcgcttcgc gctgccgttc
     1321 gaaccgcacg cgaccgggta cggccacacg tcggaagacg tgggggcggt gcgcgccgac
     1381 gccgcgttgc tgctcgcata cttcgacgcg gtgcacgaac gcacgatcgg gtatctgcgc
     1441 acgatcgaac cgaacgaaat ggaacgaatc gtcgacgatc ggtgggatcc gccggtcacg
     1501 ctcgcggtac gcctggtaag tgtgctgtcc gacgatctcc aacatgcggg gcaggccgcg
     1561 tacgtgcggg ggatcatcga gcgccgctga aatcccggga gatcggaacc acatgaaacg
     1621 ggtgctcgtc gccgtctgcg cgcttcttgc gctcatcggg ctgagcgcgt gcgacgtccc
     1681 gctcaagacc gcggccagta ccgcgaagat cgagctcctc gcaacgagca cgaacggcac
     1741 ctggcagtac gactactacc gcaacaacgc gtatccctgt tcgatcagcg ggtaccagac
     1801 gttcgtcgtc gggcggaagg tcggttcgag ctccgacgcc aggccgttgt gggtgttcat
     1861 gcacggcggc ggcgcggggt acttcgacga gaacgggaat ccgatccccg gtcggggcca
     1921 gaaggtcgaa gaggacttcg acagtttgcg ggggcgcctc acgaacggcg gattgagcaa
     1981 gaacatccgc agcgacgccg cgggcttccg gatgctcgcc gtgtcgatgt gcagccacga
     2041 cgtgtatgcc ggcatgaaca cgtccgaccc gcacaacccg aacacgacgc ccgacgggaa
     2101 gccgcgcccc acgaccgggc tgatctccac caagtccgcc atccagttca cgcaggggac
     2161 gtatccgacg accaagacgt tcctcgccgg cggcagcgcc ggatcggtcg gcacgttcaa
     2221 cgtcgcgtgg tcgatgcagc tccaggggat cgcgccggcc gggatcgtgg ccgacgcgtc
     2281 gctcatcaac gtcgaatcgc ggcaggcggc cttccagcaa ggcgtgtgca ccgaggacaa
     2341 cgatcccgcg cgcatcgcag gaatatccgg ccgagtacat ccggaccttg ccgacatcaa
     2401 caacgaagtc gacaagatcg ttgccaacgg caagctcacc gtcccgatca tgcacatctg
     2461 gaaccacggc gacacgaaca cgtgtggtgc aacacctgtc acgtgtccgt tgcgcgacgg
     2521 cacaacggtc acgatgggtc tcacggattg catgcaccga ccactgcaga tcgtgatcag
     2581 cgcgcaaggt ccgacgagca agtcgcggaa cctgccggtg tgcgtggacg ccgacgacgt
     2641 gaagggtgac tgctcgacac acgtggtcac gacgcgcgcc agcgtgctca acaccgatcc
     2701 cgcgtcgccg gccgactaca tcggcgcggt cgtcgactgg gtccacgccc gcctcgccga
     2761 cgcgtaaccc caggtcacgt gaaggactgc gcgtggctgc gccgccggcc aaccgaagac
     2821 gacgatcgac tgcccgtcag ggtcggtgac gacgagctcg tcgggcactc agcggaccga
     2881 gatcaagtcg acgacgaaca cgagtgtctc gttcgggccg atgacaccac cggcgccgcg
     2941 cttgccgtag ccgaggttcg gcgggatcac gaggcgccgc cgcccgccga cctgcatgcc
     3001 gttcacgcct tggtcccaac cggcgatcac gtcgcccgcg ccgaggcgga aggagaacgt
     3061 ctcaccgcgg tcccacgatg cgtcgaactg cttgcgggtc gaccaagagt gcccgacgta
     3121 gtgcacggtc acttcgtcgc cggccgcggc ggcgcgaccg ctgcccggct cgaggtcttc
     3181 gatcgtcagc tcaccgggcg gtgcttcgcc gggcgggaca tagacgtgcg gcttctcgtc
     3241 gttggccatg cgttgcaccc tacgacgtca gatcacgcgc aggaggagct cctggccgac
     3301 ggatgcgcac aaccggccgt ccgcggtgtg caacgtgccc cgcacggtgc cgcggccgcc
     3361 ggcattcacc aaggagtgga ggtcgtaata cacccagtcg tcgacgcgga gcggccggtg
     3421 gaaccagagc gcatggtcga ggctgacgat gccttccatc acggggtcga ggttgtgggg
     3481 ccgcgcgccg gtgaacgtca tgtcgctgat ccacgcaacg atcgccgcgt gcagcatcgg
     3541 gtcgtcgctc atcggcgccg gcagcttgaa gtacgcgcgg tgcgtcgact gtcgcgtccc
     3601 gtcggctgcc ggttcggtcg gcccggcaac ccgctcgagc cacggtccct gcccctgcca
     3661 ctcctcgagg ccgtcgaggt cgggcagcgt gtcgagcacc ggcagctcgt actcgtaccc
     3721 gtcggtgtcg gcggcgaacg agaccatcat cgtgcacgcg tgcttgcccg actggaatgc
     3781 gtcgacgcgc cgggtcgcgt acgaacgacc gtcgcgcacg cgcacgatct cgtactcgac
     3841 gggcgtgccg gcgagcaccg gccggaggaa gtagcagtgc aaggaatgtg gacgccggcc
     3901 gtcgtcggcg tcgacggtcg tcgttgccgc gaagagcgtc tgcccgagga tgaagccgcc
     3961 gaacgcgacc ggtccgaacc agtcgggaac gtcgccaacc gcgcggtcgg taccggtcgg
     4021 gcgcgcgctg acggtttcga gcaagtcggc ttgcgcggcg atcttcatct cgaggtccca
     4081 cacttcttcg tccacgccgc cggaccgtac aacgcgacgc tcttaccggt cgcgatttat
     4141 gggtacaccc gagtccatga agatcacacg cggttctctc gtgcggcggg tcgctcggca
     4201 gatgctcgcg ggcatgttca tcaatggtgg gctggatgcg ttgcgcaatc cggcgcccaa
     4261 ggtcacgcgc gccgagcgcg tcgtcgaacc gctcaacgac gcgctcggca tccagaccga
     4321 cgccgagacg ttgatcaggg ccaacggcgc cgtcatggtc gccggaggcg tcctgctctc
     4381 gaccggtcgc ctcccgcggc tggcatcgct ggtgctcgcc accaccttga tcccgacgac
     4441 gttcgccggt cacgcgttct gggccgagga agacccgcag acccgcgccc agcagcggac
     4501 ccagttcttc aagaacgcat ccatgttcgg cgggctcctc ttcgccgcgc tcgataccgg
     4561 gggtcgcccg tcgattccgt ggcgcgccaa gcgggccgcg cacaaggcgg ctgagcaggt
     4621 gagcgaacac gtcacgttgc cgcacgccgc gtaggcggct cacaatggcg gcatgaccgc
     4681 ctgcatctcc gcggattccc acgtcaccga gccgccgggt acctacgtcg accgcatcga
     4741 cgcgcgcttc aaggaccgcg cgccgcgcat gcacttcgac gagacactcg gcgatgtgat
     4801 gctcatcgac aacggtgcga cgctcgtgcc gttctggctc gtcgccgcgg ccggccgccc
     4861 gaccgaagag gtacgcctcg atacggggaa gcggttcgac gagttgcacc ggggcggttg
     4921 ggatccgcac gcccgattgg tcgaccagga caccgacggc gtgcaggccg aagtgatcta
     4981 tccctccgtc ggcatgatcc tgtg
//
LOCUS       DASUGA010000439         2239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393 k141_2625,
            whole genome shotgun sequence.
ACCESSION   DASUGA010000439 DASUGA010000000
VERSION     DASUGA010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2625"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VFX21_08690"
     CDS             <1..638
                     /locus_tag="VFX21_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX5096077.1"
                     /translation="TVPTGIILGLLLAVLAHRQLKGMGIYRTIFSSTVATSVAVASVI
                     FFTLLNPTIGLIPYWFGTSTNLLNDPTWALPMVSLTTIWQNLGLSFIIMSAGLQAVPD
                     ELIEAARVDGAGTWSRFRNVTLPLLSPTLFFAIVVGSILALQAFGQIDLLTQGGPRNH
                     TNVLVYSIYTAVFREHNEGKGAVLAVALFAITFVFTMIQITFLERRVTYER"
     gene            628..1578
                     /locus_tag="VFX21_08695"
     CDS             628..1578
                     /locus_tag="VFX21_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX5096078.1"
                     /translation="MSAELAPGFRNFLLVVAVLLVGVVVLAIVGSRAAAAAARRARVV
                     NRYILLTILAVVAAFPIYITLVNSFLEPDRIAAKPPALFAWPPVGSGYSLAWNELNVS
                     EYLQNSVVVTFVIVAGQIITGVLAAYAFAFLEFPFKRILFVVFLTTLMIPFEVVFFTN
                     LQTLATLGDNPGLTWIAPGTYGALTFPFLATGFGAFLVRQSFLSLPRDLRDAAALDGY
                     GHFRFMTRVAVPLARPAVASLALFSFFAAWNQYLWPLVAAGDDHKTIQIGLATLKNTA
                     LDQVNATFAGAILAALPLFVLLLIFQKQLVRGLTAGAVKG"
     gene            1586..>2239
                     /locus_tag="VFX21_08700"
     CDS             1586..>2239
                     /locus_tag="VFX21_08700"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5096079.1"
                     /translation="MKTKWWRAAVAMLALTAVLAACGGGDDDDDDSSSGGGGGGGNNN
                     GAAGPDLPNCPLDALEQASGPVDITLWHAMTRANLDELTRLTDQYNSSQSKVHVSLSE
                     SANYTDNLTRYTAGLGTGDLPDLVQIEDTGTQLMIDSQSVLPAASCIKADNFDTSDII
                     PRVLAYYTVKDVLYPMPFNVSNPILYYNKNAFTNAGLDPEKPPTTLDEMMEASQKIVD
                     "
BASE COUNT          357 a          800 c          665 g          417 t
ORIGIN      
        1 tcacggtccc caccgggatc atcctcgggc tgctgctcgc cgtgctcgcg caccgccagc
       61 tgaagggcat ggggatctac cgcacgatct tctcgtcgac cgtcgcgacg tcggtcgcgg
      121 tcgcgtccgt catcttcttc acgttgttga acccgacgat cgggctcatc ccgtactggt
      181 tcggcaccag cacgaacttg ctcaacgatc cgacgtgggc gctgccgatg gtgtcactca
      241 cgaccatctg gcagaacctc ggattgtcct tcatcatcat gtccgcgggc ttgcaggccg
      301 ttcccgacga actgatcgaa gccgcacgtg tcgacggtgc cggcacatgg tcgcgctttc
      361 gcaacgtcac cttgccgttg ctgtcgccga cgctgttctt cgcgatcgtc gtcggctcca
      421 tcctggctct ccaggcgttc ggccagatcg atctgctgac gcaaggcgga ccgcgcaacc
      481 acaccaacgt cctcgtctac tcgatctaca ccgcggtctt ccgcgagcac aacgaaggca
      541 agggcgcggt gctcgcggtc gcgctcttcg cgatcacgtt cgtgttcacg atgatccaga
      601 tcaccttcct cgaacggcgg gtcacctatg agcgctgagc tcgcacccgg cttccgcaac
      661 ttcctgctcg tcgtggcggt gctcctggtc ggcgtcgtcg tgctcgcgat cgtggggagc
      721 cgagccgcag ccgcagcggc gcgccgggcc cgtgtcgtca accgctacat cctcctcacg
      781 atcctggcgg tggtcgcggc cttcccgatc tacatcacgc tggtcaactc gttcctcgaa
      841 ccggatcgca tcgcggccaa gccgcccgcg ctcttcgcgt ggccaccggt cgggagcggc
      901 tattcgctcg cgtggaacga gctcaacgtc tccgagtact tgcagaacag cgtcgtcgtc
      961 acgttcgtca tcgtggcggg ccagatcatc accggtgtcc tcgccgcgta cgcattcgcg
     1021 ttcctcgagt tcccgttcaa gcgcatcctc ttcgtcgtgt tcctgacgac gctgatgatc
     1081 ccgttcgagg tcgtcttctt cacgaacctg caaacgctcg cgacgctcgg cgacaatccc
     1141 ggcctcacgt ggatcgcacc cggcacctac ggcgcgctga cgttcccgtt cctcgcgacc
     1201 ggattcggcg ctttcctcgt tcgccagtcg ttcctgtcgc tgccgcgcga cctgcgcgac
     1261 gcggccgcgc tcgacggcta cgggcacttc cggttcatga cgcgcgtcgc ggtaccgctc
     1321 gcgcggcccg cggtcgcctc gctcgcgctg ttctcgttct tcgcggcctg gaaccagtac
     1381 ctctggccgc tggtcgcggc cggcgacgac cacaagacga tccagatcgg cttggcgacg
     1441 ctcaagaaca cggcactcga ccaggtcaac gccaccttcg cgggcgcgat cctggccgcg
     1501 ctcccgctgt tcgttctcct ccttatattc cagaagcaac tggtgcgcgg cctgacggcc
     1561 ggtgcggtga agggttgatc ctcagatgaa gaccaagtgg tggcgagcgg cggtcgcgat
     1621 gctggcgctc acggcggtcc tcgccgcgtg cggcggcggt gacgacgacg acgacgacag
     1681 cagctccggt ggtgggggcg gcggcggcaa caacaacggt gcggcgggcc ccgacctccc
     1741 caactgcccg ctcgacgcgc tcgaacaggc atcgggaccg gtcgacatca cgttgtggca
     1801 cgcgatgacg cgcgccaacc tcgacgagct cacgaggctg accgatcagt acaactcgtc
     1861 acagtcgaag gtccacgtgt cactgtcgga gtcggcgaac tacaccgaca acctcacgcg
     1921 ctacaccgcc ggcctcggca ccggagacct gcccgacctc gtgcagatcg aggacaccgg
     1981 tacgcagttg atgatcgaca gccagtcggt gctgcccgcc gcgtcgtgca tcaaggccga
     2041 caacttcgac acgtccgaca tcatcccgcg cgtccttgcg tactacacgg tgaaggacgt
     2101 cctgtacccg atgccgttca acgtctcgaa cccgatcctc tactacaaca agaacgcgtt
     2161 cacgaacgcc ggcctcgacc cggagaagcc gccgacgacg ctcgacgaga tgatggaggc
     2221 gtcgcagaag atcgtcgac
//
LOCUS       DASUGA010000440         2483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1304028, whole genome shotgun sequence.
ACCESSION   DASUGA010000440 DASUGA010000000
VERSION     DASUGA010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304028"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VFX21_08705"
     CDS             complement(<1..508)
                     /locus_tag="VFX21_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096080.1"
                     /translation="MATVGAPATAAPTPPRVFAPSTPGAAPAAPPPGAFDLLPAGSKP
                     PASAPPPIAPPAPEPGSEVDTLTPAQPPPNATRPSMADVPTKYFVVASVVLLALAVWF
                     MWPRGGGGDSDSSAVKVLDPIADSDLPGVQDAIRIQAESNLHVVTQAMSMQFAQSGSV
                     ATITPDALA"
     gene            complement(656..1300)
                     /locus_tag="VFX21_08710"
     CDS             complement(656..1300)
                     /locus_tag="VFX21_08710"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX5096081.1"
                     /translation="MSEAAPGLPDSASFATAERAALAASLRKLIDVAMTSEDADSETL
                     VDVAHAVDQLAFRLGGERDKGPGYNPRSHGDYLPRSPVVGEASPLSPRLDWEIAEDGS
                     VTGRGSFGAAYEGPPGYVHGGWVACAFDEMLGIANIASGNPGMTARLTIHYRKPTPLF
                     HELRFKAWVDRLVGRRIMSRGELWDGETLTAEADGIFVQPRPEVAEQYFGPRPD"
     gene            complement(1305..2120)
                     /locus_tag="VFX21_08715"
     CDS             complement(1305..2120)
                     /locus_tag="VFX21_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX5096082.1"
                     /translation="MTEILQDLTTKHCTVERDGDVVIFTMNRPEKKNALSGDMLAGLV
                     LGYEYVDADPTVRCAILTGAGGNFSSGADLVAMGQQSDDPKVQAVMARGNNPHWKALL
                     RDYRLSKPLIAAVEGYAVAGGTEILQGTDIRVAGESAIFGVWEAKRGLFPLGGSACRL
                     PRQIPYTLAMDILLRCPPVPAPQAKEIGLIGHVVPDGQALTRARELAQEVAQNAPLSM
                     MAILRAWRETEHMTDEEAMQHQDPIGWEVFATEDAQEGPKAFAEKRAPNYKGR"
     gene            2240..>2483
                     /locus_tag="VFX21_08720"
     CDS             2240..>2483
                     /locus_tag="VFX21_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5096083.1"
                     /translation="MGGFWNYAQEDPGYLAIVEPDGTEHTAGDVLARANQVVHALRSL
                     GFRPGDTVSAVLPNGSHPMHIYLAALQAGWYYVPINY"
BASE COUNT          360 a          875 c          865 g          383 t
ORIGIN      
        1 gcgccagcgc gtccggcgtg atcgtcgcca cggatcccga ctgcgcgaac tgcatcgaca
       61 tcgcctgggt cacgacgtgg aggttcgatt cggcctggat gcggatcgcg tcctgcacgc
      121 ccggcaggtc gctgtcggcg atgggatcga gcaccttgac ggcgctgctg tcgctgtcac
      181 caccgccgcc gcggggccac atgaaccaca cggcgagtgc gagcagcacg acgctcgcca
      241 ccacgaagta cttcgtcggg acgtcggcca tcgacggtcg cgtcgcgttc gggggcggtt
      301 gggccggcgt gagcgtgtcc acctcggagc cgggctcggg cgcaggcggc gcgatcggcg
      361 gcggcgcgct cgccggtggc ttgctccccg ccggcaacaa gtcgaacgcg ccgggcggcg
      421 gcgcggcggg tgccgcgccg ggcgtcgacg gcgcgaagac gcgcggtggc gtcggggcgg
      481 cggtcgcggg cgcgccgacg gttgccactg ccggcgcgac cgacgcgttg atctgcgtcc
      541 cgcatcggcg gcacgcgccc cacgccggtg gcaccaggta cgaacagccc ggacaccgga
      601 tcaccggtca tggatcggca ttccggtgta cgagacttga aacagggccc gaagttcagt
      661 cggggcgggg cccgaagtac tgctcggcga cctccgggcg gggctgcacg aagatgccgt
      721 cggcttcggc ggtgagtgtc tcgccgtccc acagctcgcc gcgggacatg atgcgccggc
      781 caacgagtcg gtcgacccac gccttgaacc gcagctcgtg gaacaacggc gtcggcttgc
      841 ggtagtggat cgtcaaccgc gcggtcatgc ccgggttccc cgacgcgatg ttcgcgatgc
      901 cgagcatctc gtcgaacgcg caggcgaccc acccgccgtg cacgtatccg ggcggccctt
      961 cgtacgcggc gccgaacgaa ccgcgaccgg tgaccgaacc gtcctcggcg atctcccagt
     1021 cgagacgcgg cgacagcggg ctcgcctcgc cgacgacagg agaacggggc aggtagtcgc
     1081 cgtgactgcg cgggttgtag ccgggacctt tgtcgcgttc gccgccgagg cggaacgcga
     1141 gctggtcgac ggcgtgcgcg acgtcgacca gcgtctcgct gtccgcgtcc tcgctcgtca
     1201 tcgcgacgtc gatcagcttg cggagcgacg ccgcgagcgc ggcgcgttcc gcggttgcga
     1261 aggacgcaga gtcgggaagc ccgggggcgg cttcactcac gtgctcaacg gcccttgtag
     1321 ttcggggcgc gcttctcggc gaacgccttc gggccctcct gggcgtcctc ggtcgcgaac
     1381 acctcccacc cgatcgggtc ctggtgctgc atggcctctt cgtcggtcat gtgctccgtc
     1441 tcgcgccacg cgcgcaggat cgccatcatc gacagcggcg cgttctgcgc gacttcctgc
     1501 gcgagctccc tcgcacgcgt cagcgcctgg ccgtcgggca cgacgtgacc gatgaggccg
     1561 atctccttcg cctgcggcgc gggaacgggt gggcaacgca acaagatgtc catcgcgagc
     1621 gtgtacggga tctggcgcgg gagccggcac gcggaaccgc cgagtgggaa caacccgcgc
     1681 ttggcctccc acacaccgaa gatcgcgctc tcacccgcga cgcggatgtc ggtgccctgc
     1741 aggatctcgg tgccacccgc gaccgcgtag ccctcgaccg ccgcgatcaa cggcttgctc
     1801 aaccggtagt cgcgcagcaa cgccttccag tgcgggttgt tgccgcgcgc catcactgct
     1861 tggaccttcg gatcgtcgga ctgttgtccc atcgcgacga ggtccgcacc cgacgagaag
     1921 ttgccacccg cgccggtgag gatcgcgcag cgcacggtcg ggtcggcgtc gacgtactcg
     1981 tagccgagca ccagcccggc gagcatgtcg cccgagagcg cgttcttctt ctccggccgg
     2041 ttcatcgtga agatgacgac gtcgccgtcg cgctcgaccg tgcagtgctt cgtcgtgagg
     2101 tcttgcagga tctcggtcac ggccgcgcac actagaacac gttccaggtc cggagccggg
     2161 cgaccggcgc gccttgcggc caatgtgaaa cgtgttctac tttcggcggt cccggttccg
     2221 cgaggaaagg cgcgcacgca tgggtggctt ctggaactac gcacaggagg accccgggta
     2281 cctcgcgatc gtggagcccg acggcaccga gcacaccgcg ggtgacgtcc tcgcgcgcgc
     2341 caaccaagta gtgcacgcgc tgcgcagcct cgggttccgg cccggcgaca ccgtctcggc
     2401 cgtgctcccg aacggttcgc acccgatgca catctacctc gccgcgttgc aagccggttg
     2461 gtactacgtg ccgatcaact acc
//
LOCUS       DASUGA010000441         9308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1533162, whole genome shotgun sequence.
ACCESSION   DASUGA010000441 DASUGA010000000
VERSION     DASUGA010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 9308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9308
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533162"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VFX21_08725"
     CDS             <1..635
                     /locus_tag="VFX21_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEX5096084.1"
                     /translation="WTPKILELMSNVDSFIPEPTRDIDKPFLMPIEDVFTITGRGTVV
                     TGRVERGMVKVGEEIEIIGLRPTTKTTCTGVEMFRKLLDEGRAGDNIGALLRGTKKED
                     VERGQVLAKPGSITPHTEFTAQVYILTKEEGGRHTPFFTNYHPQFYFRTTDVTGTVNL
                     PEGTEMVMPGDNTEMTVELGKPIAMDEGLRFAIREGGRTVGSGRVVKIIK"
     gene            651..815
                     /gene="rpmG"
                     /locus_tag="VFX21_08730"
     CDS             651..815
                     /gene="rpmG"
                     /locus_tag="VFX21_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX5096085.1"
                     /translation="MPRSDKRIKVTLACEECKRRNYITIKSKINDRERIEMKKYCRWD
                     KRHTLHKETR"
     gene            899..1459
                     /locus_tag="VFX21_08735"
     CDS             899..1459
                     /locus_tag="VFX21_08735"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5096086.1"
                     /translation="MDEAELIGAAQSGDRDAFDELVRRTFVDTFTLARRLTGNEEDAR
                     DVVQESYLRAWRAIGKFRGDAQFSTWMYRITANASATHMKKRRRHQTEPIDEIHEPVD
                     TRPEAQPASAAEVSDDLGRITVALDDLPPKLRQLVVLKDVYGLSHEAIAEELGISVAA
                     AKVRLHRARRKLRDTLYEQGAEAHAV"
     gene            1449..1808
                     /locus_tag="VFX21_08740"
     CDS             1449..1808
                     /locus_tag="VFX21_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096087.1"
                     /translation="MRCDEVAALLPSLVDGIDEANVAVQRHVDTCLRCQAEVARYRKL
                     VRTLELLRTRYVEPTPGLLGETLVALSEAGERGVLRTYVTSRRFAYGGAAVGTAALAA
                     GTAALIIARSRRRAAVA"
     gene            1956..2028
                     /locus_tag="VFX21_08745"
     tRNA            1956..2028
                     /locus_tag="VFX21_08745"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1989..1991,aa:Trp,seq:cca)
     gene            2072..2344
                     /gene="secE"
                     /locus_tag="VFX21_08750"
     CDS             2072..2344
                     /gene="secE"
                     /locus_tag="VFX21_08750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX5096088.1"
                     /translation="MNRQSKRIMAKQGTDKPRASASRPQSPTKERVGPRQYYREVMGE
                     LRKVAWPTKSEIVNSSIVVIIGVVVMAALIFGFDWASLHLVDIIFG"
     gene            2344..3141
                     /gene="nusG"
                     /locus_tag="VFX21_08755"
     CDS             2344..3141
                     /gene="nusG"
                     /locus_tag="VFX21_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX5096089.1"
                     /translation="MTDIFGTSPTTTDPDREVVYEDDAAPAPISDETRAEVADPALDE
                     TVAETEVEAPAELDAEELGDAELIEVAAVTEAEQSPYDRPGRWYVVHTYAGYENKVKS
                     NLASRVRSMNVEDRIYEVVIPMEDVIEFKNGRKVVVQKKVFPGYLLVRMGLDDDAWYV
                     VRNTPGVTGFVGSGAKPTPLSRKEVEDILGTGEKEGVGVPGPEKKARPRLEYESGEQV
                     RVVTGPFADFNGVISEIDVDRSKLKVLVNIFGRETPVELEFADVAKL"
     gene            3157..3585
                     /gene="rplK"
                     /locus_tag="VFX21_08760"
     CDS             3157..3585
                     /gene="rplK"
                     /locus_tag="VFX21_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX5096090.1"
                     /translation="MAKRRVAAIVKIQIQAGAATPAPPVGTALGPHGINIMDFCKAYN
                     AQTEGQRGTVIPAEITIYEDRTFSFITKTPPTPVLLRQAAGIEKGAQAPARETVATVT
                     KAQVREIAATKMPDLNAIDIEGAMAQVAGTARSMGIDVAE"
     gene            3669..4361
                     /gene="rplA"
                     /locus_tag="VFX21_08765"
     CDS             3669..4361
                     /gene="rplA"
                     /locus_tag="VFX21_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX5096091.1"
                     /translation="MPGKKYVDASRRFDKTHLHQPDEAFELVKSMGTKKFDETVEAAF
                     RLGVDPRKADQMLRGTVSLPSGTGKDVRVAVFATGDAAREAEEAGADVVGADDLVDRI
                     SKGVLDFDVAIATPDLMGQVGKLGRVLGPRGLMPNPKTGTVTNDVGKTVGEFKGGKVE
                     YRTDRFGNVHVPIGKVSFSTEALTKNYGAVLDEIIRAKPAAAKGRYLKAVTTSSTMGP
                     GVKINPEARVEH"
     gene            complement(4371..5570)
                     /locus_tag="VFX21_08770"
     CDS             complement(4371..5570)
                     /locus_tag="VFX21_08770"
                     /inference="COORDINATES: protein motif:HMM:NF015541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5096092.1"
                     /translation="MPGVRRSVRALALAAGLVLASCSSGDDDAADRAGLPPATTPARA
                     TSTTEPAPAAPPALVAAERIDVPDVTGAAWRVTYMSQRVDGVPVEVTGLVVRPVGAAP
                     PGGFPVVAWGHGTIGVGDACIQMNSFDGHLGIPALQQRLDAGYVVAATDYEGIGGPGP
                     HPYLVAESEARSIFDIARVLPQIEGVDASTRVVLVGFSQGGHAVLAAAERRARLAPDL
                     DVVGVAALATNGDLGESVRAIFRRRDLAEFGMLLAVGWGDTYPELEATDVIAPAGADA
                     ADAARDGKCLGELQPFLGDATVGDLWVQSPTALAPWVARVRENDLHENAIDLPVFVAG
                     GGDDPLAVPSSVNRLVERLCGAGLAVDDHRYTGAGHGATVDASITDVHEWIAARFRGE
                     VAPSSCA"
     gene            5683..5997
                     /locus_tag="VFX21_08775"
     CDS             5683..5997
                     /locus_tag="VFX21_08775"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX5096093.1"
                     /translation="MSFSNEIPSISGRQAVDAQFESVGKVTDVLFGDVSSEPRWAVVK
                     TGLLGGEHYVPLSNSYIDQRGRLVVPFDKTTVKHAPKAHGDHVMTRDLADELQSYWGV
                     AA"
     gene            complement(6010..6387)
                     /locus_tag="VFX21_08780"
     CDS             complement(6010..6387)
                     /locus_tag="VFX21_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096094.1"
                     /translation="MITPHVRAFLDGLHELLAEHHQVMLTTDGDATALVRVEGSPIEI
                     EVTDRTVRLRYGGEWQLLHDHAFALQVVGALLDGRVEIEIHHGLLWRTTRSYLDGSPR
                     PFVTTRMPVPSLRLHTERRRVVS"
     gene            complement(6387..7166)
                     /locus_tag="VFX21_08785"
     CDS             complement(6387..7166)
                     /locus_tag="VFX21_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5096095.1"
                     /translation="MTDDAARAHANLVNFVRWQRALEPDAHVLDADGVVATAGTIDFP
                     SNRMAVRASEVLSPEHWVATADRFFTGIGKSACIFLRLRADDDLLAPLVAHGYAAYEQ
                     SPEMVCRAPLAEREPPDGFTLRLATTPDDIAAFAAVAAEAFKHLGFLPDTIIDPMRNT
                     DVMLGPSVMVSIAERDGAVVAGAVAILLGDEPDSYVGWVSCADSARGNGLGDHVTRLV
                     TNESFRRGARLCTLEASRYGESTYRRMGYEELYRYQMLIKV"
     gene            7380..8057
                     /gene="rplJ"
                     /locus_tag="VFX21_08790"
     CDS             7380..8057
                     /gene="rplJ"
                     /locus_tag="VFX21_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX5096096.1"
                     /translation="MPSKAPAGSAQHERKTAVVADIKSRLDDADAAVLTEYRGLTVSQ
                     IADLRAELRKAATDYKVFKNTLARRAAVDAGLEDLVANLEGPVAIAFVRKDGGDAVTA
                     AKALREFAKTNDNLVLKGGILGPRILTTKDVEALADVPPRDVLLARLAGGFQAPLTKA
                     AGLFQAFTRNFAYGLKAYIDQRPPEAAPVADAVVEDAVVADAPVEDAPVADAAPENEI
                     NEAGETE"
     gene            8057..8449
                     /gene="rplL"
                     /locus_tag="VFX21_08795"
     CDS             8057..8449
                     /gene="rplL"
                     /locus_tag="VFX21_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX5096097.1"
                     /translation="MATMSTTELLDVFKNMTVLELNEFLKAFEEEFGVTAAAPVAVAA
                     VGAPAAGGGGEAAAEEKDEFDVILNAAGDKKIQVIKVVRELTSLGLKEAKDLVDGAPK
                     PVLEKVSKDDADKAKTKLEESGAQVEVK"
     gene            8724..>9308
                     /locus_tag="VFX21_08800"
     CDS             8724..>9308
                     /locus_tag="VFX21_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX5096098.1"
                     /translation="MGVTLARLASRERLSFSKLREVKDLPNLIEVQTDSFNWFLEGGV
                     AEVLRDISPIEDFTGQLKLELTDHVFDPPKHSEDECRERDMTFARPLFVTARFMNAQT
                     GEIKEQTVFMGDFPMMTDRGTFIINGTERIVVSQLVRSPGVYFGVNADKTAPEKEIVD
                     AKMIPSRGAWLEFEVDKRDVVYVRIDRKRKQPVTI"
BASE COUNT         1668 a         3188 c         3139 g         1313 t
ORIGIN      
        1 aatggactcc caagatcctc gaactgatgt ccaacgtcga cagcttcatc ccggagccga
       61 cgcgcgacat cgacaagccg ttcctcatgc cgatcgaaga cgtcttcacc atcacgggtc
      121 gcggcacggt cgtgaccggc cgcgtggagc gcgggatggt caaggtcggg gaggagatcg
      181 agatcatcgg tctgcgcccg acgacgaaga ccacgtgcac cggcgtcgag atgttccgca
      241 agctgctcga cgagggccgc gccggcgaca acatcggtgc gctcttgcgc ggcacgaaga
      301 aggaagacgt cgagcgcggt caggtgctcg ccaaacccgg cagcatcacg ccgcacacgg
      361 agttcaccgc gcaggtgtac atcctcacca aggaagaggg tgggcggcac acgccgttct
      421 tcacgaacta ccacccgcag ttctacttcc gcacgaccga cgtgaccggc acggtgaacc
      481 tccccgaggg caccgagatg gtgatgccgg gtgacaacac cgagatgacc gtcgagctcg
      541 gcaagcccat cgccatggac gaaggtctgc ggttcgccat ccgtgagggt ggccgcaccg
      601 tcggctccgg ccgtgtcgtc aagatcatca agtagcaggg aaggcaactc gtgcccaggt
      661 ccgacaagcg catcaaggtc acgctcgcct gcgaagagtg caagcggcgg aactacatca
      721 cgatcaagtc caagatcaac gaccgcgagc gcatcgaaat gaagaagtac tgccgctggg
      781 acaagcggca cacgctccac aaggagacgc gttagccgtg acggcccccg cactccgggg
      841 gccgtaacct tttcgggccc gtgggagtcg aaggggccac caccctggaa gggggctcgt
      901 ggacgaagcc gaactcatcg gcgccgccca atccggtgac cgtgacgctt tcgacgagct
      961 cgtgcgccgc acgttcgtcg acaccttcac gctcgcccgc cgcctgacgg ggaacgagga
     1021 ggacgcacgc gacgtggtgc aagagtcgta tctccgggcc tggcgggcca tcgggaagtt
     1081 ccgcggtgac gcgcagttct cgacgtggat gtaccgcatc accgcgaacg catcggcgac
     1141 gcacatgaag aagcgccggc ggcatcagac cgagcccatc gacgagatcc acgaacccgt
     1201 cgacacccgg cccgaagccc agccggcgag cgcggccgaa gtgtccgacg atctcgggcg
     1261 gatcaccgtc gcccttgacg acctgccgcc gaagctgcgt caactcgtgg tcttgaagga
     1321 tgtctacggc ctctcgcacg aagccattgc cgaggagctc ggcatctccg tcgcggccgc
     1381 gaaggtgcgg ttgcatcggg cgcggcgcaa gctgcgcgac acgctgtacg agcaaggagc
     1441 agaagcccat gcggtgtgac gaggttgccg cactgttgcc gagcctcgtc gacgggatcg
     1501 acgaggccaa cgtcgccgtg caacggcacg tcgacacgtg tttgcgatgc caggccgaag
     1561 tggcccgcta tcggaagctc gtgcggacgc tcgaactgtt gcgtacgcgc tacgtcgaac
     1621 cgacgccggg actgctgggt gagacgctcg ttgcgctgag cgaagccggc gagcgcggtg
     1681 tgttgcgcac ctacgtcacc agccgtcgct tcgcgtacgg cggtgccgcg gtcggtaccg
     1741 cggcactcgc ggccggcacc gcggcgctga tcatcgcccg ctcccgccgc cgcgccgccg
     1801 tcgcctgaca gctcgccgtt cctcggaccc ggtccggtcg gcgtgtcgac cagggctcgg
     1861 atccgtgccg acacgggccg agttctccga tccaggcgcc tcgggcggcc ggatatcccg
     1921 ctgctatcct gttatgcctg gcccccaagg cccggagggc agtagctcaa ctggcagagc
     1981 tccggtctcc aaaaccggcg gttgggggtt cgagtccctc ctgccctgca agatcccttt
     2041 cccgccgcgc cgaggcacca tgaacgcccc gatgaaccga caatccaagc gcatcatggc
     2101 caagcagggc accgacaagc cgcgcgcgtc ggcttcgcgt ccgcaatcac cgaccaagga
     2161 acgggtcggg ccgcgccagt actaccgcga ggtcatgggt gagctgcgca aagtggcgtg
     2221 gcccaccaag tccgagatcg tcaactcgtc gatcgtcgtg atcatcggcg tcgtcgtgat
     2281 ggctgcgctc atcttcggat tcgactgggc ctcgctccac ctcgtcgaca tcatctttgg
     2341 ctaatgactg acatcttcgg aacttccccc accaccacgg atcccgatcg tgaggtcgtc
     2401 tacgaggacg acgccgcgcc cgcgccgatc tccgacgaga cgcgcgccga ggtcgccgac
     2461 cccgcgctcg acgagacggt tgcggagacc gaggtcgagg caccggccga gctcgacgcc
     2521 gaggagctcg gcgacgcgga gctgatcgag gtcgccgccg tcaccgaggc cgagcagagc
     2581 ccgtacgacc ggcccggccg gtggtacgtc gtgcacacct acgccggcta cgagaacaag
     2641 gtgaagtcga acctcgccag ccgcgtgcgg tcgatgaacg tcgaagaccg catctacgag
     2701 gtcgtcatcc cgatggaaga cgtcatcgag ttcaagaacg gccgcaaggt cgtcgtgcag
     2761 aagaaggtct tcccgggcta cctgctcgtg cgcatgggcc tcgacgacga cgcgtggtac
     2821 gtcgtgcgca acacgccggg tgtcaccggg ttcgtcggca gcggcgcgaa gccgacgccg
     2881 ttgtcgcgca aggaagtcga agacatcctc ggcaccggcg agaaggaagg cgttggcgtt
     2941 cccggccccg agaagaaggc gcgcccgcgc ctcgagtacg agtccggcga gcaggtgcgg
     3001 gtcgtcaccg ggccgttcgc cgacttcaac ggtgtgatca gcgagatcga cgtcgaccgt
     3061 tcgaagctga aggtgctcgt gaacatcttc ggtcgtgaaa cgcccgtcga gctcgagttc
     3121 gcggacgtcg cgaagctcta agagacagaa agaccaatgg caaaacgtcg agttgctgca
     3181 atcgtcaaaa ttcagatcca ggccggcgcg gcgacgcccg cgccgccggt cggtaccgcg
     3241 ctcggtccgc acggcatcaa catcatggac ttctgcaagg cgtacaacgc gcagacagaa
     3301 ggccagcgcg gcaccgtgat ccccgccgag atcacgatct acgaagaccg cacgttcagc
     3361 ttcatcacca agacgccgcc cacgccggtg ctgttgcgcc aggccgcggg catcgagaag
     3421 ggcgcgcagg cgccggcgcg cgagaccgtc gcgacggtga cgaaggcgca ggtccgcgag
     3481 atcgcggcaa cgaagatgcc cgacctcaac gcgatcgaca tcgaaggtgc gatggcgcag
     3541 gtcgcgggca cggcccgttc gatgggcatc gacgtcgccg agtaagcaac acgcacgacc
     3601 cgcggccgga ggacctcgcc ggccgagctc gggatgccga gggagcttgc aacacgagga
     3661 gcgcagcaat gccgggcaag aagtacgtcg acgccagccg tcgtttcgac aagacccacc
     3721 tccaccagcc cgacgaagcg ttcgagctgg tgaagtcgat gggcaccaag aagttcgacg
     3781 agaccgtcga agccgcgttc cgcctcggcg tcgacccccg caaggccgac cagatgctgc
     3841 gcggcaccgt gtcgctgccg tcgggcaccg gcaaggacgt gcgcgtcgcg gtgttcgcca
     3901 ccggtgacgc ggcccgtgag gccgaagagg cgggtgccga cgtcgtcggc gccgacgacc
     3961 tggtcgaccg catctccaag ggcgtcctcg acttcgacgt cgcgatcgcg acgcccgatc
     4021 tcatgggtca agtcggcaag ctcggtcggg tgctcggtcc gcgcggcctc atgccgaacc
     4081 cgaagaccgg caccgtcacc aacgacgtcg gcaagacggt cggcgagttc aagggcggca
     4141 aggtcgagta ccgcaccgac cgtttcggca acgtgcacgt gccgatcggc aaggtcagct
     4201 tctcgaccga ggcgctcacc aagaactacg gcgcggtgct cgacgagatc atccgggcca
     4261 agcccgccgc cgccaagggt cgctacctca aggcggtcac gacgtcgtcg acgatgggcc
     4321 cgggcgtcaa gatcaacccc gaagcccgcg tcgagcacta gccgagatcg tcacgcgcac
     4381 gacgacggcg cgacctcgcc gcggaagcgc gcggcgatcc actcgtggac gtcggtgatc
     4441 gatgcgtcca cggtcgcgcc gtggccggcg ccggtgtagc gatggtcgtc gacggcgagc
     4501 ccggcgccgc acagtcgctc gacgagccgg ttgaccgacg acggcacggc gagcggatcg
     4561 tcgccgccac ccgcgacgaa cacgggaagg tcgatcgcat tctcgtggag gtcgttctcg
     4621 cgaacgcggg ccacccacgg cgcaagcgcg gtgggtgact gcacccacag gtcgccgacg
     4681 gtcgcgtcgc cgaggaacgg ctgcagctcg ccgaggcact tgccgtctcg cgccgcgtcc
     4741 gcggcgtcgg cgccggcggg cgcgatgaca tcggtggcct cgagctccgg gtaggtgtcg
     4801 ccccatccga ccgcgagcaa catcccgaac tcggcgagat cgcgacggcg gaagatcgcg
     4861 cgcaccgatt caccgaggtc gccgttcgtc gcgagggcgg cgacgccgac cacgtcgaga
     4921 tcaggcgcca gccgagcgcg ccgctcggcc gcggcgagca ccgcgtgccc gccctgcgaa
     4981 aagcccacga gcacaacgcg cgtcgacgcg tcgacgcctt cgatctgggg gaggacgcgc
     5041 gcgatgtcga agatcgagcg cgcttcgctc tcggccacca ggtacgggtg cgggccgggt
     5101 ccgccgattc cctcgtagtc ggtcgccgcg acgacgtatc ccgcgtcgag tcgttgctgc
     5161 aacgcgggga tgccgaggtg accgtcgaac gagttcatct ggatgcacgc gtcaccgacc
     5221 ccgatcgttc cgtgacccca cgcgacgacg gggaacccac cgggtggcgc cgcgccgacg
     5281 ggccgcacga cgagtcccgt gacctcgacg ggcacgccgt cgacgcgttg cgacatgtac
     5341 gtcacccgcc aggccgcgcc ggtgacgtcc ggcacgtcga tgcgctcggc ggcgacgagg
     5401 gccggcggcg cggcgggcgc cggttcggtc gtcgacgtcg cacgcgcggg ggtcgtcgcc
     5461 ggcgggaggc cggcacggtc ggcggcatcg tcgtcgcccg aagagcagga cgcgagcacg
     5521 aggccggcgg cgagcgccag cgcgcggacc gatcgtctga cgcccggcac gctcagacgg
     5581 tagcgatcgg cgcgatcgac atcgcgtcaa gcgcctcgcg ccgatgaggg cgatacttcc
     5641 gacatatccg aagcgcgtgc attggctggg tacgcgcgcg gcatgagttt cagtaacgag
     5701 attccctcca tcagtgggcg ccaggcagtc gacgcgcagt tcgaatcggt cggcaaggtg
     5761 accgacgtgt tgttcggtga cgtctcctcc gagccgcgat gggcggtcgt gaagaccggc
     5821 cttctcggtg gcgagcacta cgtcccgctg agcaactcgt acatcgacca gcgcggccgg
     5881 ctcgtcgtcc ccttcgacaa gacgaccgtc aagcacgcgc cgaaggcgca cggcgatcac
     5941 gtcatgacgc gcgacctcgc cgacgagctc cagagctact ggggcgtcgc ggcctagtcg
     6001 ggcaccggct caggagacga cgcgccgtcg ctccgtatgg aggcggagcg acggcaccgg
     6061 catgcgggtc gtgacgaacg gacgcggcga cccgtcgaga tagctgcgcg tcgtccgcca
     6121 gaggaggccg tgatggatct cgatctcgac ccggccgtcc aacaacgccc ccacgacctg
     6181 cagcgcgaac gcgtggtcgt gcaggagctg ccattcgccg ccgtaccgca accgcacggt
     6241 tcggtcggtg acttcgatct cgatcggcga cccttcgacg cgcaccagtg ccgtcgcatc
     6301 gccgtccgtg gtcaacatca cctggtggtg ttccgcgaga agctcgtgca gcccgtcgag
     6361 gaacgcccgg acgtgcggcg tgatcatcag accttgatga gcatctggta ccggtagagc
     6421 tcttcgtagc ccatgcgccg gtaggtcgat tcgccgtagc gcgacgcctc gagggtgcac
     6481 aagcgagcgc cgcgccggaa ggactcgttg gtcacgagcc gggtgacgtg gtcgccgaga
     6541 ccgttgccgc gcgccgagtc cgcgcacgaa acccatccca catacgagtc gggctcgtcg
     6601 ccgagcagga ttgcgacggc accggcgacg acagcgccgt ctcgttcggc gatcgacacc
     6661 atgaccgacg ggccgagcat cacgtcggtg ttgcgcatcg ggtcgatgat cgtgtccggc
     6721 aggaagccca agtgcttgaa cgcctccgcg gcgactgccg cgaacgcggc gatgtcgtcg
     6781 ggggtcgtcg cgagccggag ggtgaagccg tcgggcggtt cgcgctcggc caacggcgcg
     6841 cggcacacca tctcggggct ttgctcgtag gcggcgtagc cgtgcgcgac gaggggggcc
     6901 agcaagtcgt cgtcggcacg caatcggagg aagatgcacg cgctcttgcc gatgcccgtg
     6961 aagaagcggt cggccgtcgc gacccagtgc tcgggcgaca gcacctccga tgcccgcacg
     7021 gccatccggt tcgacgggaa gtcgatcgtc cccgccgtcg cgacgacacc atccgcgtcg
     7081 aggacgtggg cgtcgggctc gagcgcccgc tgccaccgca cgaagttcac gaggttggcg
     7141 tgggcgcggg ctgcgtcgtc ggtcacgccc cgaccgtaga ccggccgccg ggaccggggc
     7201 cgaggccggt cggctatatt ggtgcgtcca tctgaacacc gaagacctcc ggtctcgaca
     7261 cgaggtaatg ggctgcggcc cgccggacga ggtgaccttg ggagcgccga cccgccacca
     7321 cgcgggggcc gcgcggctcg tgaggcgacc tcgcccgagc cgcacccgag gagcatgaca
     7381 tgcccagcaa ggcacccgcc ggcagcgcgc agcacgaacg caagaccgcc gtcgtcgccg
     7441 acatcaagag ccgcctcgac gacgcggacg ccgccgtgct caccgaatac cgcggcctga
     7501 ccgtgtccca gatcgccgac ctgcgggccg agctccgcaa ggcggcgacc gactacaagg
     7561 tcttcaagaa cacgctcgcc cgccgggccg cggtcgacgc cggactcgaa gacctcgtcg
     7621 caaacctcga gggccccgtc gccatcgcgt tcgtgcgcaa ggacggcggc gatgccgtca
     7681 ctgcggccaa ggcgctgcgc gagttcgcga agacgaacga caacctcgtg ctgaagggcg
     7741 gcatcctcgg cccgcgcatc ctgacgacca aggacgtcga ggcgctcgcc gacgtgccgc
     7801 cgcgcgacgt gctgctcgcc cggctcgccg gcggcttcca ggccccgttg acgaaggccg
     7861 cgggcctgtt ccaggcgttc acccgcaact tcgcctacgg gctgaaggcg tacatcgacc
     7921 agcgcccgcc cgaagccgcg ccagtcgcgg acgccgtcgt cgaagacgcc gtcgtggcag
     7981 atgcaccagt cgaggacgcg cccgtcgcgg acgcggcacc cgagaacgag atcaacgaag
     8041 caggagaaac cgagtaatgg caaccatgag cacgaccgag ctgctcgacg tcttcaagaa
     8101 catgacggtc ctcgagctga acgaattctt gaaggccttc gaagaggagt tcggcgtcac
     8161 cgccgcagct ccggtcgcag tggccgccgt cggcgcgccc gccgcgggtg gcggcggcga
     8221 ggccgcggcc gaggagaagg acgagttcga cgtcatcctc aacgccgccg gcgacaagaa
     8281 gatccaggtc atcaaggtgg tccgcgagct gaccagcctg ggcctgaagg aggccaagga
     8341 cctcgtcgac ggcgccccca agcccgtctt ggagaaggtc tcgaaggacg acgcggacaa
     8401 ggccaagacc aagctcgagg agtcgggcgc ccaggtcgag gtcaagtagc tccggacaaa
     8461 agtcctggtc accacccggg cgcgggagat tttccggccc gggtggttgt caggagccgg
     8521 gtcccgtcgt atcctccgcg ccagcaaatg cggaggatct ctacggtccc gcgccacggt
     8581 attggcagcc ggcatggccc agcgctatgc tgggcgtagc cgtgtctgcc cgtccgtgcc
     8641 ttgtgagcaa ttcttatctc ctcgacgcgc gcccgaatcc gggtcgcgcg tcgttgtgtt
     8701 tccccgcagc gtgacccacc ccgattggag tgacgttggc tcgtctcgcg tcccgtgagc
     8761 gtttgtcctt ctcgaagttg cgtgaggtca aagacctccc gaacctgatc gaagtccaga
     8821 ccgattcgtt caactggttc ctcgaaggcg gtgtcgccga agtgctccgc gacatctcgc
     8881 cgatcgagga cttcaccggc cagctcaagc tcgagctgac cgaccacgtc ttcgacccgc
     8941 cgaagcactc cgaagacgag tgccgcgagc gtgacatgac gttcgcgcgc ccgctgttcg
     9001 tcaccgcgcg cttcatgaac gcgcagaccg gtgagatcaa agagcagacg gtcttcatgg
     9061 gcgacttccc gatgatgacc gaccgcggca cgttcatcat caacggcacc gagcgcatcg
     9121 tcgtctcgca gctcgtccgg tcgccgggcg tgtacttcgg cgtcaacgcc gacaagaccg
     9181 cgcccgagaa ggagatcgtc gacgccaaga tgatcccgag ccgcggcgcg tggctcgagt
     9241 tcgaggtcga caagcgcgac gtcgtctacg tccgcatcga ccgcaagcgc aagcagccgg
     9301 tcacgatc
//
LOCUS       DASUGA010000442         2199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1064922, whole genome shotgun sequence.
ACCESSION   DASUGA010000442 DASUGA010000000
VERSION     DASUGA010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064922"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1488
                     /locus_tag="VFX21_08805"
     CDS             <1..1488
                     /locus_tag="VFX21_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004945860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5096099.1"
                     /translation="RPDRGEVARTPPAATIGYLPQEPERRADETAGAFLARRTGVADA
                     ALAFEVASLALAKAEPGADDAYATALDHYLAIGAADFDARVATVLDQVGLDADILGRA
                     TTELSGGQAARLSLAAILLSRFDVFLLDEPTNDLDFDGLALLEDFVQHLAGGVVIVSH
                     DRAFLDRTITSVLELDDHSHQATLFAGGWRAYLDERAIARRHAEEAYADYQDKRSQLV
                     ARSQQQREWATQGVSNVKKSGEKDKFIRHFRTSSSERLAGKARATDRALERLEANAVE
                     KPWEGWELRMEVASAPRSGDLVARLAGAVVERGAFRLGPIDLDIAWGERVAILGANGS
                     GKTTLLGALLGDLPLRAGEQRLGPSVVVGRLDQARARFESDATLLASFQADTGADASV
                     CRSTLAKFGLGAEHVLRPAATLSPGERTRAVLAWFTLVGVNCLVLDEPTNHLDVPAIE
                     QLELALTSFGGTVLLVTHDRQLLDAVTLTREIQLAHGRVIADVPR"
     gene            complement(1485..>2199)
                     /locus_tag="VFX21_08810"
     CDS             complement(1485..>2199)
                     /locus_tag="VFX21_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017306374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5096100.1"
                     /translation="GIGAATAAAFNQAGAAVIGLDLDAQIVARSNDAFLGVECDITDG
                     QALDRAFDAAVQRFGGLDVVVCNAGMFPPSAPIASLSDDLWRRVMAVNLDANVALLRT
                     AAPLLANSPRGGRVVVNASKNVAAPGPGAAAYSASKAALTQLARVAALEWAPQGVRVN
                     VVHPNAVFDTGIWDDATLASRAESYGLSLEEYKRNNLLGVEVASTDVAAVIVALCSAT
                     FAKTTGAQIPIDGGNERVV"
BASE COUNT          323 a          796 c          768 g          312 t
ORIGIN      
        1 cggcccgatc gcggcgaggt cgcgcggacg ccgcccgcgg ccacgatcgg ctatttgccg
       61 caggagcccg agcggcgcgc cgacgagacc gccggcgcgt tccttgcccg ccgcaccggc
      121 gtggccgacg cggcactggc gttcgaggtg gcgtcgctcg cgctggccaa ggccgagccg
      181 ggcgcggacg acgcctacgc gaccgcgctc gaccactacc tcgcgatcgg cgccgccgac
      241 ttcgacgccc gcgtcgcgac ggtcctggac caagtcgggc tcgacgccga catcctcggg
      301 cgcgcgacca cggagctctc cggcgggcag gcggctcgcc tgtcgctggc cgcgatcctg
      361 ctgtcgcgtt tcgacgtctt cctcctcgac gagccgacca acgacctcga cttcgacggg
      421 ctcgcgctgc tcgaagattt cgtgcagcac ctggcgggcg gcgtcgtcat cgtctcccac
      481 gaccgtgcgt tcctcgaccg cacgatcaca tcggtgctcg agctggacga ccattcacac
      541 caggcgacgc tcttcgccgg tggctggcgc gcgtatctcg acgaacgcgc gatcgcacgc
      601 cggcatgccg aggaggccta cgccgactac caggacaagc gctcgcaact cgtcgcgcgc
      661 agccaacaac agcgcgagtg ggccacgcag ggcgtcagca atgtgaagaa gagcggcgag
      721 aaggacaagt tcatccgtca cttcaggacg agctcgagcg aacggctcgc gggcaaggcg
      781 cgggcgaccg accgcgcgct cgagcgcctc gaagcgaatg cggtcgagaa gccgtgggaa
      841 gggtgggaac tccgcatgga ggtcgcgagc gcgccgcgca gcggcgatct cgtcgcgcgc
      901 ctcgcggggg cggtcgtcga gcgcggtgcg ttccggctcg ggccgatcga tctcgacatc
      961 gcgtggggcg aacgggtcgc gatcctcggc gccaacggca gcggcaagac gacgctgctc
     1021 ggcgcgttac tcggtgatct accgctgcgc gcgggcgagc aacgcctcgg cccctcggtc
     1081 gtcgtgggcc gtctcgacca ggcccgcgcc cgtttcgaat cggacgccac gctgctggcg
     1141 tcgttccaag ccgacacggg ggccgacgcg tccgtgtgcc ggtcgacgct cgccaagttc
     1201 gggctcggcg ccgagcacgt gttgcggccc gcggcgacgc tctcgccggg tgaacgcacc
     1261 cgcgcggtgc tcgcgtggtt cacgctcgtc ggcgtcaatt gcctcgtgct cgacgagccg
     1321 acgaaccacc tcgacgtccc cgcgatcgaa caactcgaac ttgcgttgac gtccttcggc
     1381 ggcaccgtgc tgctggtgac ccatgaccgg cagctgctcg acgcggtcac gctcacgcgc
     1441 gagatccagc tcgcgcacgg ccgcgtcatt gcggacgtgc cgcgctaaac cacgcgttcg
     1501 ttgccgccgt cgatcgggat ctgggcgccg gtcgtcttcg cgaacgttgc cgagcagagc
     1561 gcgacgataa ccgcggccac gtcggtgctc gcgacctcga cgccgagcag gttgttgcgc
     1621 ttgtactcct ccaacgacag tccgtagctc tcggcgcggg acgcgagcgt cgcgtcgtcc
     1681 cagatgccgg tgtcgaacac cgcattcgga tggacgacat tgacgcggac gccctggggc
     1741 gcccactcga gcgcggcgac gcgcgccagt tgcgtcagcg ccgccttgga cgccgagtac
     1801 gcggccgcgc cgggacccgg tgcggcgacg ttcttcgacg cgttcacgac cacccgcccg
     1861 ccgcgcggac tgttcgcgag cagcggcgcg gcggtgcgca gaagcgcgac gttggcgtcg
     1921 aggttcacgg ccatgacccg ccgccacagg tcatcgctca gggaggcaat cggcgccgac
     1981 ggcgggaaca tgccggcgtt gcacacgacg acgtcgaggc ccccgaaacg ttgcaccgcc
     2041 gcatcgaagg cacggtccag cgcctgcccg tcggtgatgt cgcactcgac gccgaggaac
     2101 gcgtcgttcg agcgcgcgac gatctgcgcg tcgaggtcga ggccgatgac tgcggcaccc
     2161 gcttggttga acgcagccgc cgtcgcggca ccgatgccg
//
LOCUS       DASUGA010000443         3695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_38327, whole genome shotgun sequence.
ACCESSION   DASUGA010000443 DASUGA010000000
VERSION     DASUGA010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38327"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..722)
                     /locus_tag="VFX21_08815"
     CDS             complement(18..722)
                     /locus_tag="VFX21_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096101.1"
                     /translation="MLGRRILVPATVAAAIAAGGVAGAVLGVPGISGAQESPTTVAPG
                     PEDAPAPPEGGPGLMFHHRGGPMMGGDMLDAAATALGMTTDELLAQLKDGKSIADIAQ
                     EKNVELDKVVDAIVTEMLSNLPDEAELRDRVEPWLDGEMPRPTAPDGESPRAWRMHPD
                     GAPDGKMFRGPAGPQLDAAAAALGMSVEDLRSELRDGKTIADVAEEKNVDVQKVIDAM
                     VEADRDRITDFVNGEN"
     gene            870..1562
                     /locus_tag="VFX21_08820"
     CDS             870..1562
                     /locus_tag="VFX21_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5096102.1"
                     /translation="MVTGPRILVVDDEPNITDLVATALRYEGFDVAVAGNGMDAIREV
                     GSFRPDLLVLDVMLPDRDGFELLKRLRADSLHVPVVFLTARDSTEDKVKGLTVGGDDY
                     VTKPFSLEELVARVRAVLRRTNGTSADSGRLVVGDLEMNDDTHEVWRGSTRIDLTPTE
                     YKLLRYLMLNARRALSKAQILDHVWEYDFGGDANVVETYISYLRKKLDPLGPPLIHTI
                     RGIGYSVRPPEQ"
     gene            1563..2945
                     /locus_tag="VFX21_08825"
     CDS             1563..2945
                     /locus_tag="VFX21_08825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5096103.1"
                     /translation="MSLRTRLSLLLVVLAAVGLVVAGYVTHRATRTYLVDRVDGQLED
                     SLKSPQVLFPNNSFGAEGRVARTLPPGTFAELRDSFGNAFESTDRVLQNPPELPDEIE
                     PGKIIELDNPHFRVTAGIVNVRAGPQVGQALMVVAIPMRDVDETLSHLRFVELVVAAI
                     VLAALALLGWLIVRRELRPLEQMAETAGAIAAGDLTQRVEYENETNEVGRLGVALNAM
                     LGQIEAAFAEREASESKLRQFVGDASHELRTPLTSIRGYAELFRRGAADRPEDLDKAM
                     RRIEEEAVRMGVLVDDLLLLARLDQGRPLQRDTVDLAQLATDAVDDARAATPARDITY
                     VANGAVTVTGDEARLRQVLANLLSNACAHAGPDAHVACTVHTDNGDAVIEVRDDGPGM
                     SADDAAHVFERFWRADKARTRSSGGAGLGLSIVAAIAEAHGGKAEVETAPGQGAVFRV
                     RVPAATPTTL"
     gene            complement(2932..>3695)
                     /locus_tag="VFX21_08830"
     CDS             complement(2932..>3695)
                     /locus_tag="VFX21_08830"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX5096104.1"
                     /translation="PEKYPLGRAFMALTGWQQYRLLAAEEFPPVTMVPEDVNLVDVLH
                     VLGHVGMTAYLGVTEVAQPREGETFVVSAAAGGVGSLAGQIARLRGARVIGIAGGPDK
                     CKWVVDELGFDACIDYKHEDVAARLKELVPGGVDIYFDNVGGELLDTVLRRLAVRGRV
                     VLCGDISTYDTATPATPARALKYVMGKRARMEGFNTLDHWTRYLEAADQLSQWNAEGK
                     IKHREHVLDGLDRAPEALVRLFSGDHLGKLVVKVS"
BASE COUNT          578 a         1313 c         1224 g          580 t
ORIGIN      
        1 cccgacccga cgcgttgcta gttctcgccg ttgacgaagt cggtgatgcg gtcgcggtcg
       61 gcctcgacca ttgcgtcgat gaccttctgc acgtcgacgt tcttctcctc ggcgacgtcg
      121 gcgatcgtct tgccgtcgcg cagctcgctg cgcagatcct cgaccgacat gccgagcgcg
      181 gcggccgccg cgtcgagctg cgggccggcc ggaccccgga acatcttgcc gtcgggtgcg
      241 ccgtcggggt gcattctcca cgcacggggg ctctcgccgt ccggcgcggt cggtcgcggc
      301 atctcgccgt cgagccaggg ctccaccctg tcgcgcagct cggcttcgtc gggcaggttc
      361 gacagcatct cggtgacgat cgcgtcgacg accttgtcga gctcgacgtt cttctcctgc
      421 gcgatgtcgg cgatgctctt gccgtccttc agctgcgcga gcaactcgtc cgtcgtcatg
      481 ccgagcgccg tggcggccgc gtcgagcatg tcgccgccca tcatcggacc gccacggtgg
      541 tggaacatga ggcccggacc gccctccggc ggcgcgggcg catcttcggg tccgggcgcg
      601 accgtcgtcg gcgactcctg cgcacccgag atcccgggga cgccgagcac cgccccggcg
      661 acgccgccgg ccgcgatggc ggccgcgaca gtggcaggaa cgaggatgcg acgacccagc
      721 atggagagct tccttccgaa cggagcaccg gattcctgtg agcatcgacg acctacctgg
      781 caatgacctg ggaaaactga gaacgcaccc agcttcagac cacagagacc tcccaggggg
      841 ctcacagctc attgcgcgac tatgagcgca tggtgaccgg cccgcgcatt ctcgtcgtcg
      901 acgacgaacc gaacatcacg gacctcgtcg cgactgcgtt gcgctacgag ggattcgacg
      961 tcgcggtcgc cggcaacggc atggacgcca tccgggaggt cgggtcgttc cgccccgacc
     1021 tgctcgtgct cgacgtgatg ctccccgacc gcgatggttt cgagttgctg aagcggttgc
     1081 gcgcggactc gttgcacgtc cccgtcgtct tcctcactgc ccgcgactcg accgaggaca
     1141 aggtgaaggg cctgacggtc ggcggcgacg actacgtcac caagccgttc agcctggagg
     1201 agctggtcgc gcgcgtgcgc gccgtcctgc gccgcacgaa cggcaccagt gccgactccg
     1261 gccgcctcgt ggtcggcgac ctcgagatga acgacgacac gcacgaggtc tggcgcgggt
     1321 cgactcgtat cgacctgacg ccgaccgagt acaagctgct gcgctacctc atgttgaacg
     1381 cgcgccgcgc gctgtcgaag gcgcagatcc tcgaccacgt ctgggagtac gacttcggcg
     1441 gcgatgccaa cgtcgtcgag acgtacatca gctacttgcg caagaagctc gatccgctcg
     1501 ggccgccgct gatacatacc attcgcggca tcggatacag cgttcggcca ccggaacagt
     1561 agatgtcgct gcggacgcgg ctgtcgctcc tgctcgtcgt gctggccgcg gtcgggctcg
     1621 tcgtcgcggg ctacgtcacg caccgcgcca cgcgcacgta tctcgtcgac cgcgtcgacg
     1681 gacagctcga agactccctc aagtcaccgc aggtgctgtt ccccaacaac agcttcggcg
     1741 cggagggccg cgtcgcccgt acgctgccgc ccggcacctt tgccgagctg cgtgactcct
     1801 tcggcaacgc gttcgagagc accgaccgcg tgctgcagaa tccgcccgaa ctgccggacg
     1861 agatcgagcc gggcaagatc atcgagctcg acaacccgca cttccgcgtc acggccggca
     1921 tcgtgaacgt gcgcgccggt ccgcaggtcg ggcaggcgct catggtcgtg gccatcccga
     1981 tgcgcgacgt ggacgagacg ctgtcgcatc tccggttcgt cgagctcgtc gtcgccgcga
     2041 tcgtcctcgc cgcgctcgcg ttactcggtt ggctgatcgt gcgccgcgaa ctgcgcccac
     2101 tggagcagat ggccgagacc gccggggcga tcgcggccgg tgacctcacc caacgcgtgg
     2161 agtacgagaa cgagacgaac gaggtcggcc gcctcggggt cgcgctgaac gcgatgctcg
     2221 ggcagatcga ggccgcgttc gccgagcgcg aagcgtcgga gtcgaagctg cgccagttcg
     2281 tcggcgacgc atcccacgag ctgcgcacgc ccctgacctc cattcgcggc tacgcggagc
     2341 tgttccggcg cggtgcggcc gaccgccccg aagacctcga caaggcgatg cgccgtatcg
     2401 aggaagaagc ggtccgcatg ggggtgctgg tcgacgacct gctcctgctc gcccgcctcg
     2461 accaaggccg cccgctccaa cgcgacaccg tcgacctcgc gcaactcgcg acggacgcgg
     2521 tcgacgacgc ccgcgccgca actcccgcgc gcgacatcac atacgtcgcg aacggcgcgg
     2581 tgaccgtcac gggcgacgag gcacgattgc gccaggtgct cgccaacctc ttgtcgaacg
     2641 cgtgcgcgca cgctgggccc gacgcgcacg tcgcctgcac cgtccacacc gacaacgggg
     2701 acgcggtcat cgaggtgcgt gacgacggtc ccggcatgag tgccgacgat gcggcgcacg
     2761 tgttcgagcg cttctggcgc gcggacaagg cgcgcacccg gtcgagcggc ggcgcgggac
     2821 tcggcctgtc gatcgtcgcc gccatcgcgg aagcgcacgg cggcaaggcc gaagtggaga
     2881 ccgcacccgg gcagggtgcg gtgttccggg tgcgcgtgcc ggccgcgacg cctacgacac
     2941 tttgacgacg agcttgccga ggtggtcgcc gctgaacagg cggaccagcg cctcgggcgc
     3001 gcggtcgaga ccgtcgagca cgtgctcgcg gtgcttgatc ttgccctcgg cattccactg
     3061 cgagagctgg tcggccgctt cgagatagcg cgtccaatgg tcgagggtgt tgaaaccctc
     3121 catccgcgcc cgcttcccca tcacatactt gagcgcgcgt gccggtgtcg ccggcgtcgc
     3181 ggtgtcgtag gtcgagatgt caccgcacaa cacgacgcga ccgcgcaccg ccaaccgccg
     3241 caacacggtg tcgaggagct cgccgccgac gttgtcgaag tagatgtcga cgccccccgg
     3301 taccagctcc ttcaaccgcg ccgcgacgtc ttcgtgcttg tagtcgatgc acgcgtcgaa
     3361 gccgagctcg tcgacgaccc acttgcactt gtcgggtccg ccggcgatcc cgatgacgcg
     3421 tgcgccgcgc agcctggcga tttgccctgc gagtgatccc acgccgcccg cggcagcaga
     3481 cacgacaaac gtttcgccct ctcgaggctg ggcaacttcg gtgacaccga gatacgccgt
     3541 catcccgacg tggccgagca cgtgcaggac gtcgacgaga ttcacgtctt cgggcaccat
     3601 cgtgacgggc gggaactctt cggcggcgag caaccgatat tgctgccagc cggtaagcgc
     3661 catgaacgcg cgcccaagcg gatacttctc gggat
//
LOCUS       DASUGA010000444         2803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1809214, whole genome shotgun sequence.
ACCESSION   DASUGA010000444 DASUGA010000000
VERSION     DASUGA010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1809214"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(301..1035)
                     /locus_tag="VFX21_08835"
     CDS             complement(301..1035)
                     /locus_tag="VFX21_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5096105.1"
                     /translation="MPSIAYVLPAHNEERALPEFVPPLALRLKELPGSEIIIVENGSS
                     DTTGAVAEQLATECSSADVRVRAESSAKGYGNAVRRGIEVATAERVMLTAADLPFGFS
                     DLDAALALEPLPEVVIGSKAHKQSKVNVNLQRKVMSKTFWALRKVLLHVDVGDSQGTI
                     LIERDLGQSLLPELQSGAYFFSTELIVLASRTGARVVEVPVDYSNPRRDSKVKPIHDS
                     VQVLKDMWTLRTRLRTPAVQGPSSKR"
     gene            complement(1043..1990)
                     /locus_tag="VFX21_08840"
     CDS             complement(1043..1990)
                     /locus_tag="VFX21_08840"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5096106.1"
                     /translation="MRIGVIGAGGMGANHARVIAESEVADLAMVVDRDTDRAADLARQ
                     FGAPVVVDPSEVARHCDAVVVATSTPTHLDVALPLLEGGMPLLIEKPLAETAADAEKL
                     IDASARVGVPLMCGFVERFNPALVTALSLIDEPVVNLHAMRHSPHNPYVSASVVQDLL
                     IHDIDLALRANGGTVLPDVRDTNWTPPNNSGRSEVVECVLKFESQMVANLSASRWGQR
                     KIREVRIVTDHRLVEVDLLRVNVSVYRNISQSIVEGGGPYRAETVMDVPYVRHRGEPL
                     ALQLSAFVDLVRKGDATTIDAERASIMPPHIVAATMEGL"
     gene            2078..>2803
                     /locus_tag="VFX21_08845"
     CDS             2078..>2803
                     /locus_tag="VFX21_08845"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX5096107.1"
                     /translation="MTSPHIPISRVELGPEVEALVLEVVRSGHLAQGPMVERLERTFC
                     DLVGTAHAVAVGNGTLALVAAIQALGIEAGSEIVTSPFTFVATVNAALEAGARVRFAD
                     IDLSDFNVNLDEVAHAITPATSVLMPVHLYGHPAGIGPLASLAEQHGLALIEDACQSH
                     GASVDGKVVGSFGIGCFSLYATKNVTTGEGGIVTTNDAQLADRLRVLRNQGMRARYQY
                     EMAGHNYRMTDLQAAIGVPQMEQL"
BASE COUNT          483 a          925 c          923 g          472 t
ORIGIN      
        1 gatctcgcgg ttgctggcac attggttgcg cgcgcacgag ctcttcgaac aggaccgacc
       61 gcacgtgcga cagctgaccc tcgtgcgata cgaggatctg gttcgcgatg tcgccggcac
      121 cctcgctgac gtgtttcggg cgatcgaggt cgaaccgttc gttccgaacg cgcacgtgcg
      181 ttctacgaca aacgaccgct acctcaaacg gttccgccac tcgcgcatct tcccggtcgc
      241 accgcgggtc gcggccaacc ggctccgctt cgaccgacgg ctgcggcgcc tcggctacgg
      301 ctatcgctta gacgacggac cttgaacggc gggcgttcgg aggcgtgttc gcagggtcca
      361 catgtccttg aggacctgga cgctgtcgtg gatcggcttg accttggagt cgcggcgcgg
      421 gttgctgtag tcgacgggaa cctccacgac acgcgcgccc gtgcgggacg cgagcacgat
      481 cagttccgtc gaaaagaagt aggcaccgct ctgcagttcg ggcagcaatg actggccgag
      541 gtcgcgttcg atgaggatcg tgccctggct gtcgccgaca tcgacgtgca gcagcacctt
      601 gcgcagcgcc caaaaggtct tcgacatgac cttgcgttgc aagttcacgt tgaccttcga
      661 ctgcttgtgc gccttcgacc cgatcacgac ttccggcagc ggctcgagcg cgagcgccgc
      721 atcgagatca ctgaatccga acggcagatc cgcggcggtg agcatgacgc gttcggcggt
      781 tgcaacctcg atgccgcgcc gcacggcgtt gccgtaaccc ttggcggagg actcggcccg
      841 cacgcgcacg tccgcactcg aacattcggt cgcgagctgt tcggcgacgg cgccggtcgt
      901 gtcgctcgaa ccgttctcga cgatgatgat ctcgctgccg ggaagttctt tcagacgaag
      961 cgcgagcggt ggcacgaact cgggcagcgc gcgctcctcg ttgtgcgccg gcaggacata
     1021 agcgatcgac ggcaccgtcg gatcacagac cttccatggt cgccgccacg atgtgcggcg
     1081 gcatgatcga cgcccgttcg gcatcgatcg tcgtcgcgtc gcctttgcgg accaggtcga
     1141 cgaaggcgga gagctgcaat gccagcggct cgccccggtg ccggacgtag ggcacgtcca
     1201 taacggtctc ggcgcggtac ggaccaccgc cttcgacaat cgactggctg atgttgcggt
     1261 agaccgacac gttcacgcgc aggagatcga cttcaacgag ccggtggtcg gtgacgatgc
     1321 gaacttcgcg aatcttgcgc tgaccccagc gtgacgccga aaggttcgca accatttgcg
     1381 actcgaactt caacacgcac tcgaccactt cgctgcggcc gctgttgttg ggcggcgtcc
     1441 agttcgtgtc gcgcacgtcg gggagtacgg ttccgccgtt cgcgcgcaac gcgagatcga
     1501 tgtcgtgaat caagaggtct tgcacgacac tcgccgacac gtaggggttg tgcggcgagt
     1561 ggcgcatggc gtgcaggttc acgaccggct cgtcgatgag cgagagcgcg gtgacgagcg
     1621 cgggattgaa ccgttcgaca aacccgcaca tgagcggcac gcccacgcgc gctgaagcat
     1681 cgatgagctt ctcggcgtcg gcggcggttt cggcgagcgg cttttcgatc agcagcggca
     1741 tgccgccttc gagcagcggg agcgcaacgt cgaggtgggt cggcgtcgac gtcgcgacca
     1801 cgaccgcatc gcagtggcgg gcgacctcgg acggatcgac gacgaccggc gcgccgaact
     1861 ggcgcgccag atcagccgca cgatcggtgt cgcggtcgac gaccatcgcg aggtcggcga
     1921 cctccgactc cgcaatcacc cgagcatggt tcgctcccat gccgccggcg ccgatgacgc
     1981 cgatccgcaa ctggctgatg acgggcctcc tcaggaatgc ggcggtatgc ccgacaaggg
     2041 taccgctgta gaattgatcg accgctcggg ccttcccatg acctcaccgc acatacccat
     2101 cagccgggtc gaactgggcc ccgaggtcga ggcgctggtc ctcgaggtgg tccgatccgg
     2161 ccatctggcc cagggcccca tggtcgagcg cctggaacgc acgttctgcg atctggtcgg
     2221 gaccgcgcac gcagtggcgg ttggcaacgg cacgctcgcg ctcgtcgcgg cgattcaggc
     2281 actcggcatc gaagccggat ccgagatcgt gacgtcgccg ttcacgttcg tggcgacggt
     2341 gaacgccgcg ctcgaagccg gtgcgcgcgt gcggttcgcc gacatcgatc tcagcgactt
     2401 caacgtgaac ctcgacgagg tggcacacgc aatcacgccg gcgacgagtg tgctcatgcc
     2461 tgtgcatctg tacggccacc cggccgggat cggaccgctc gcgtcactgg ccgaacagca
     2521 cggcctcgcg ctgatcgagg acgcgtgcca gtcgcacggc gcgagcgtcg acggcaaggt
     2581 cgtcggctcc ttcggcatcg gttgcttcag cctctacgcg accaagaacg tcacgaccgg
     2641 cgagggcggc atcgtcacca ccaacgacgc ccagcttgcc gatcgactgc gcgtgttgcg
     2701 gaaccagggc atgcgggccc gctaccaata cgagatggcg ggccacaact accgcatgac
     2761 cgacctgcag gccgcgatcg gagtaccgca gatggaacaa ctc
//
LOCUS       DASUGA010000445         2262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1201922, whole genome shotgun sequence.
ACCESSION   DASUGA010000445 DASUGA010000000
VERSION     DASUGA010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201922"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2200
                     /locus_tag="VFX21_08850"
     CDS             <1..2200
                     /locus_tag="VFX21_08850"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEX5096108.1"
                     /translation="VAAFLPAAVFAIAERRRWHWLAAPLLTAAAGLWLAIFVDSPDET
                     VAGIPTGAALTTFRADITDAPHVLRSATVPVVPIGAALVLAVVATLVCALATELIARR
                     LDAPLGAIGPSVALFVAVSALGSGRWAPTTGIYAGVVVLYLISLQHAEMTARRTWFQS
                     SRSRRSQYATGGIVAGVVVVLLALTLGPLFPGARGAAWINYRSLGAGQGASVLNTPSP
                     LVSIAAKLAGDRSENEVFRVKTDDKLGYYWRVIALDSFSRDDWSLNADRKSASSLRGP
                     TNAPGSKLVDQSFTLSRTIDPYWLPAAYRPIDIDLEGANVLPQSSSLFLNGNLANTRY
                     AVKSEIPGPSLEKLQGVTFADLQGQRELTELPDSFSERARGKADEKTAGKSTPYDIAA
                     ALEAYFQDPNEFTYDTSVDLRSSTNAIDAFLFEQRRGFCEQFAAAFAELARAKGIPSR
                     IAVGYQQGTFDKKSNEWVVREKDAHAWPEVWLGEEIGWYAFEPTPSRFNGTTGRGDAE
                     ANPGQNPANPGDPTTTVPGTTPETTPGSTPQTAPPPNQINVDPESNTAAKPKSTGEKV
                     LTGILVVAGLFFVLITTTIIVLVVRATRRTKRRRNDPDPRRRVLGAWTEALERLTAAG
                     VIRRPSATSLEFALRQAPADGAGDAGPPLMELARLHTAALYAADVPTDEEAEQAWSCV
                     DQIDAAVKNRVGRVERWKNRIRAMRQDRPDRDTEPEDADEVETREPVGVG"
BASE COUNT          374 a          805 c          756 g          327 t
ORIGIN      
        1 ggttgccgcc ttcctgccgg cggccgtatt cgcgatcgcc gaacgccgcc ggtggcactg
       61 gctcgcggca ccgctcctga cggctgcggc gggattgtgg cttgcgatct tcgtcgattc
      121 gcccgacgaa acggtcgccg gcatcccgac cggcgccgcg ctcacgacgt tccgcgccga
      181 catcaccgac gcaccccacg tgctgcgcag cgccacggtc ccggtcgttc cgatcggcgc
      241 cgcgctcgtg ctcgcggtcg tcgccaccct ggtgtgcgcg ctggcgacgg agttgattgc
      301 gcgccggctc gacgcaccgc tcggcgcgat cggtccgagc gtcgcgctct tcgttgccgt
      361 ctcggccttg gggagcggcc ggtgggcacc gacaaccggc atctatgccg gcgtcgtcgt
      421 gctctatctc atctcgctgc aacacgccga gatgacggca cgccgaacgt ggttccagag
      481 ttcgcgttcg cgccgctcgc aatacgcgac cggcggcatc gtcgccggag tcgtcgtggt
      541 cttgctcgcg ctcacgctcg gcccgttgtt ccccggcgct cgcggtgcgg cctggatcaa
      601 ctaccggtcg ctcggcgccg gccagggcgc gagcgtgctg aacacgccgt caccgctcgt
      661 ttcgatcgcg gcgaagcttg cgggcgaccg gtccgagaac gaagtgttcc gggtcaagac
      721 cgacgacaag ttgggctact actggcgcgt catcgcgctc gacagcttct cgcgcgacga
      781 ctggagcttg aacgcagatc ggaagtcggc atcgagtttg cgcggcccga cgaacgcccc
      841 cggttcgaaa ctcgttgacc agtcgttcac tctgagccga accatcgatc cgtactggct
      901 gcccgctgcg taccggccga ttgacatcga tctcgagggc gcgaacgtgc ttccccagtc
      961 gtcgtcgctg ttcctgaacg gcaaccttgc caacacgcgc tacgcggtga agtcagagat
     1021 tcccggaccg agcctcgaaa agctgcaagg ggtgacgttc gcggacctgc agggccagcg
     1081 cgagctcacc gaactgcccg acagcttctc cgaacgggcc cgcggcaagg ccgacgagaa
     1141 gacggccggc aagtccacgc cctacgacat cgcagccgcg ctcgaggcgt acttccagga
     1201 ccccaacgag ttcacgtacg acacgtcggt tgacctgcgg agctcgacga acgcaatcga
     1261 cgcgttcctg ttcgaacagc ggcgcgggtt ctgcgagcag ttcgccgccg cattcgccga
     1321 gttggcgcga gccaagggca tcccgtcgcg catcgccgtc ggctaccagc agggcacgtt
     1381 cgacaaaaag agcaacgaat gggtcgtgcg cgagaaggac gcgcacgcgt ggcccgaggt
     1441 ctggctgggc gaagaaatcg gctggtacgc gttcgagccg accccgagcc ggttcaacgg
     1501 caccaccggc cgcggtgacg ccgaggcgaa cccgggccag aacccggcga acccgggcga
     1561 cccgaccacc acggtccccg ggaccacgcc ggagacgacg ccgggttcga cgccgcagac
     1621 cgcgccgccg ccgaatcaga tcaacgtcga tcccgagagc aacactgccg ccaagccgaa
     1681 gagcacgggc gaaaaggtgt tgaccggcat cctcgtcgtg gccggcctgt tcttcgtgtt
     1741 gatcacgacg accatcatcg tgctcgtcgt acgcgcgacg cgccgcacca agcggcgccg
     1801 caacgatccc gacccgcgcc ggcgtgtgct cggcgcgtgg accgaagcgc tcgaacgcct
     1861 gaccgccgcg ggcgtgatcc gtcggccatc ggcaacgtca ctcgagttcg cgctgcgcca
     1921 agcgccggcc gacggtgccg gcgacgcggg cccgccgctc atggaactcg cgcgtctgca
     1981 caccgcggcc ttgtatgcgg ccgacgtgcc gaccgacgag gaagcggagc aggcatggag
     2041 ctgcgtcgac cagatcgacg ccgccgtgaa gaaccgggtc ggacgggtcg agcggtggaa
     2101 gaaccggatc cgcgcgatgc gccaggaccg gcccgaccgc gacacggagc cggaagacgc
     2161 cgacgaggtc gagacccgag agccggtcgg cgtcggctga ttcagccgga cgcggccggt
     2221 gccaccagct gttccacgaa ggcagcccgc tcggcgccga ac
//
LOCUS       DASUGA010000446         2948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_283144, whole genome shotgun sequence.
ACCESSION   DASUGA010000446 DASUGA010000000
VERSION     DASUGA010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283144"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VFX21_08855"
     CDS             <1..385
                     /locus_tag="VFX21_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5096109.1"
                     /translation="ANNVVPDRCSIVVNRRYAPDRDLETAVGEVVALCEGADEIEVIN
                     RSPAAPPNLTAHLVAALIGHGPLPVRPKLGWTDVARFAARGIPACNFGPGDPELAHTA
                     TEQVTGADLRHCATILGAFLGVPEA"
     gene            616..1740
                     /locus_tag="VFX21_08860"
     CDS             616..1740
                     /locus_tag="VFX21_08860"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX5096110.1"
                     /translation="MLVALALVRDGGDLPLAVQLLLAGCALVPWMPGVRMRVASPWFV
                     FFGLVPPMVLLMTGAHPVLFGLLALTTARVATYGRVLYAALFAGAGLGFVVLWARELD
                     GANWFVWKSYIELGLALGWALRGQRLLVVRAQETAAERAAIAAVEERRRIARDIHDTL
                     AHSLTVMMVHLNSARLQLADDPEGVAESLDELAALGRASLDEARRSVGLLSGSPVIEP
                     VDPTSAAAAVEELVGSFRRAGVDVELRTDVAMEHVGLLAEAPTTLWGASYRVIQESVA
                     NAAKHAPGTAIDIGIDIDDSGVHIEVVNALAERVLALDVPSGGHGILGMRERVALLQG
                     EITAGVEHGRWVVRAVLPLSAAAEARRSPARRSRVLGRVS"
     gene            1737..2417
                     /locus_tag="VFX21_08865"
     CDS             1737..2417
                     /locus_tag="VFX21_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5096111.1"
                     /translation="MIKLLLADDQPLVRMGLRRIFDRRHGFDVIAECGDGEEAVELAT
                     RLRPDVVVMDMRMPGTDGAEAMRRLRQVADAPPVLVLTTYDDDDTVHEAITAGAAGFA
                     LKDSVAEDLHRAVREIANGNGWVDFTVARPLLKTIGEMVPPPERRAKLNLLTERELHV
                     LKLLGRGKTNSEIASELCVSTGTVKTHVGSILAKLGLRDRAAAIVFAFEHGLVKPGGR
                     AEAVSGGR"
     gene            complement(2528..>2948)
                     /gene="lexA"
                     /locus_tag="VFX21_08870"
     CDS             complement(2528..>2948)
                     /gene="lexA"
                     /locus_tag="VFX21_08870"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX5096112.1"
                     /translation="LEPTTGAAAERRPMVLVPLVGDVAAGTGVLAAENVEEVHSLPED
                     FTGDGDLFMLRVRGDSMIDVGIYDGDFVVVRSQPDADNGDIVVAGIPGEEATVKTFLR
                     KRGKIVLRPANERLDDMVFDGDEINIYGRVVTVLRRL"
BASE COUNT          412 a          999 c         1050 g          487 t
ORIGIN      
        1 ggcgaacaac gtcgttccgg atcgctgctc gatcgtggtg aaccgccgtt acgcccccga
       61 tcgcgacctc gagaccgcgg tcggcgaggt cgtggcgctg tgcgagggcg cggacgagat
      121 cgaggtgatc aaccggtcgc cggccgcgcc accgaacctc accgcgcacc tcgttgccgc
      181 cttgatcggg catggtccgc tgccggtgcg gcccaagctg ggctggaccg acgttgcccg
      241 cttcgccgct cggggcatcc cggcgtgcaa cttcgggccc ggcgatcccg agctcgccca
      301 caccgcgacc gagcaggtca cgggcgcgga ccttcgccac tgcgcgacga tactgggtgc
      361 cttcctcggc gtgcccgagg cgtagacgcc gctccccgcg gttcccccga ccggcagatg
      421 tggcctctgt ctccagacag agaacgggcc gatcgcgtca ttgcaccttc gtgacatatg
      481 gggttactgt cgaaaaacgg gctggagggg cgtgccttgg gcggtaggcg gatcctggcg
      541 gggttccaag agcagacggc cggagcgatc ggcgttccga cctggatgaa tgcgggcgca
      601 agtctcaccg cattcgtgct ggtggccctc gcgctggtgc gagacggcgg cgaccttccc
      661 ctcgcggtgc agctcctcct cgcgggctgt gccctcgtgc cgtggatgcc cggcgtgcgg
      721 atgcgcgtcg ccagcccctg gttcgtcttc ttcggtctcg tgccgccgat ggtgctgctg
      781 atgacgggcg cgcaccccgt gttgttcggg ctgttggcgc tgaccaccgc gcgggtcgcg
      841 acctacgggc gcgtgctgta cgccgcgctc ttcgccggtg ccggcctcgg cttcgtcgtg
      901 ttgtgggccc gtgagctcga cggcgcgaac tggttcgtgt ggaagtcgta catcgagctc
      961 ggcctcgcgc tcggatgggc gctgcgcggc cagcggttgc tggtcgtgcg cgcgcaggag
     1021 acggccgccg agcgggccgc gatcgccgcg gtcgaggagc gtcgccgcat cgcacgcgac
     1081 atccacgaca cgctcgcgca ctcgttgacc gtcatgatgg tgcacctcaa cagcgcgcgg
     1141 ctgcagctcg ccgacgaccc cgaaggcgtc gccgagtcgc tcgacgagct cgccgcgttg
     1201 gggcgggcca gcctcgacga ggcgcgtcgt tccgtcggcc tgctctcggg cagtccggtc
     1261 atcgagcccg tcgacccgac cagcgcggcg gccgcggtcg aggagctggt cgggtcgttc
     1321 cggcgcgcgg gcgtcgacgt cgagctgcgc accgatgtcg cgatggagca cgtcggactg
     1381 ctggccgagg caccgacgac gctgtggggt gcgtcctacc gcgtgatcca ggaatcggtg
     1441 gcgaacgcgg cgaagcacgc gccgggaacg gcgatcgata tcggcatcga catcgacgac
     1501 tccggcgtgc acatcgaagt cgtcaatgcg ctcgccgaac gtgtgctcgc actggatgtt
     1561 cccagcggcg gtcacggaat actcggtatg cgggagcgtg tcgcgcttct ccagggggag
     1621 atcaccgccg gcgtcgaaca tgggcgttgg gtcgtcaggg ctgtgttgcc gctcagtgcc
     1681 gcggccgagg cccgccgttc acctgcacgc cgttcacgcg ttctggggag agtgtcatga
     1741 tcaagttgct gttggccgac gatcaaccgc tcgttcgcat ggggctgcgc cggatcttcg
     1801 atcgccggca cgggttcgac gtcatcgccg agtgtggcga cggtgaagag gcggtcgagc
     1861 tcgcgacgcg cttgcgaccc gatgtcgtcg tgatggacat gcgcatgccc gggaccgacg
     1921 gtgccgaagc gatgcggcgc ctccgccagg tggccgacgc gccgccggtg ctcgtgctca
     1981 ccacctacga cgacgacgac accgtgcacg aggcgatcac cgcgggcgcg gcgggtttcg
     2041 cgctcaagga ctcggtcgcc gaagacctgc accgggccgt gcgcgagatc gccaacggca
     2101 acggttgggt cgacttcacg gttgctcgtc ccttgctgaa gacgatcggc gagatggtgc
     2161 cgccgcccga gcggcgagcg aagctcaact tgttgaccga gcgcgagctg cacgtgttga
     2221 agctgctggg ccggggcaag accaactccg agatcgcgtc ggagctgtgc gtgtcgacgg
     2281 ggaccgtgaa gacccacgtc ggcagcatcc tcgccaagct ggggcttcgc gaccgcgcgg
     2341 ccgcgatcgt gttcgcgttc gagcacggcc tcgtgaagcc gggcggccgc gccgaagcgg
     2401 tcagcggcgg ccgctaaagg caccggctcg ttcttgtgat ctagaccgcc atatatggcc
     2461 atctagctca caagaagctg ctgcttcccc ctgcccgcgc cctcggccga actgccgcgg
     2521 cccggtgcta gagccggcgc aagaccgtga cgacgcggcc gtagatgttg atctcgtcgc
     2581 cgtcgaacac catgtcgtcg agacgctcgt tggcgggccg caggacgatc ttgccccgct
     2641 tgcgcaggaa cgtcttgacc gtcgcttcct cgccgggaat gcccgcgacg acgatgtcgc
     2701 cgttgtcggc atcgggctgg gagcgcacga cgacgaagtc gccgtcgtag atgccgacgt
     2761 cgatcatcga gtcgccccgc acccgtagca tgaacaggtc gccgtcgccg gtgaagtctt
     2821 cgggcagtga gtggacctct tcgacgttct cggccgcgag cacgcccgtg ccggccgcga
     2881 cgtcgcccac gagcgggacg agcaccatcg gccgccgctc ggccgcggca cccgtggtgg
     2941 gctcgagc
//
LOCUS       DASUGA010000447         3809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1778411, whole genome shotgun sequence.
ACCESSION   DASUGA010000447 DASUGA010000000
VERSION     DASUGA010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1778411"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VFX21_08875"
     CDS             complement(<1..267)
                     /locus_tag="VFX21_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5096113.1"
                     /translation="MIDQVSVDVGGHRAVHDVTLELRAGELVALVGPNGAGKSTLLGT
                     LAGDLQASTGRVHIDGSPVNEWSARELAMRRPVLPQQVTVSFPFA"
     gene            complement(336..1409)
                     /locus_tag="VFX21_08880"
     CDS             complement(336..1409)
                     /locus_tag="VFX21_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX5096114.1"
                     /translation="MAAPADLEDVVAAPRRVSPTVLIGGLVVALVGGCVVAAAVGAFS
                     IPITDILGSIAHRVGLPFGPQPDPTAESVLWDIRFPRIALAVVVGAALGCAGASMQGT
                     FGNPLAEPGIVGVSSGAALGAVAGIALGFAALGTWSLTAVAFVGGLVTVAVVYAASRS
                     DGRTEVVTLILTGIAVNAFTGAVIGLLTFFADDAELRSITFWSLGSVAQATWPKVAVV
                     APVAVAGIVLATRRARSLDLLALGDRAAVHVGVDVDRLRLAMLTIVAVLTASAVAVSG
                     IILFVGLVVPHLVRMVAGPGHRLLLPASALGGAALLVVADLIARTIAAPAEVPLGVLT
                     ALIGSPFFFWLLRRTRARQGGWA"
     gene            complement(1393..2382)
                     /locus_tag="VFX21_08885"
     CDS             complement(1393..2382)
                     /locus_tag="VFX21_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018853633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5096115.1"
                     /translation="MRHRARTLAVALAACSLLLACGTAGSATGDGARYDEAYVLRPIA
                     DDPAPALPVTVPSADGREVTVTDADRIVPLSGSLAEVVFALGLGDHVVARDVSTTFPE
                     AEHLPLVTHAHDVSAEGVLSVGATVVLAQPDTGPPEALDQIRAAGVPVVVFDLPTQLE
                     DVSTRIHAIAAALGVPTEGAALAERTQAAIADAQRAIPHDEPPPRVAFLYMRGTAGVY
                     LIAGDGSGADSMIEAAGAIDAGTAMGLDKPFTPITSEALVEAAPDVILMTTTGLESVG
                     GIDGLLQIPGIAQTPAGRERRVITQEDGVLYSFGARTPAALATLIGEIHGRAG"
     gene            complement(2379..3233)
                     /locus_tag="VFX21_08890"
     CDS             complement(2379..3233)
                     /locus_tag="VFX21_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096116.1"
                     /translation="MSTMHAVRRAGTFLLALGLVVLPASPGGAETATDGTRTLTVSQA
                     TGLDPAGQMVRVTGSGYDTFKGIYVAFCAAPTPGQAPSPCGGGVDMAGGSGASHWISS
                     NPPPYGQGVATPYGPGGSFDVTVAVSAMLNATVDCRVVQCAIVTRNDHTRASDRAQDV
                     VIPVHFASPATPPPASSGNPPTGASSGASPAPESPTAPDAGVAPVDGAAPDDALASST
                     TTAAEAEATTTTPSGGEVRGGELAAASVDDGGGGSSAAVIGVSIAVAAIVLGFTAFGV
                     HRWRRQRA"
     gene            complement(3230..>3809)
                     /locus_tag="VFX21_08895"
     CDS             complement(3230..>3809)
                     /locus_tag="VFX21_08895"
                     /inference="COORDINATES: protein motif:HMM:NF013306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEX5096117.1"
                     /translation="VGSACARALLRGEIEREAYAAFVGQLGFVYGELERAGDAMRTDP
                     IAGPFVCDALRRGPALAEDLQFWLGDEWRNRIERLPATEQYCGRLQDVAFTWPGGFVA
                     HHYTRYLGDLSGGQAIARVVKRAFGLNSREGARFYDFPDIDDTKAFKASYRELLDRAP
                     WDEAEQARIIDESSEAFRLNGALFAQLAEYVS"
BASE COUNT          624 a         1338 c         1355 g          492 t
ORIGIN      
        1 ggcgaacgga aacgacaccg tcacttgttg cggcagcacg ggacgacgca tagccagctc
       61 acgagctgac cactcgttca ccggcgaacc gtcgatgtgc acgcggcccg tcgatgcttg
      121 caggtcgccc gcgagcgtgc ccagcaacgt cgacttgccg gcaccgttcg gaccgaccaa
      181 cgccacgagc tcgccggcgc gcaactcgag cgtcacgtcg tgcacggcac gatgcccgcc
      241 gacgtccacc gagacctggt cgatcatcgc caaggtcgag ccggccggcg cccgcgcggg
      301 caggcgaggg gcgcgacgcc acgacgcgcc ggacatcacg cccaaccgcc ctgacgggct
      361 cgcgtacggc gtaggagcca gaagaagaac ggactcccga taagcgcggt gaggacaccg
      421 agaggtacct cggcgggtgc ggcgatcgtg cgggcaatga gatcagccac caccaacaag
      481 gccgcgccac ccagcgccga tgccggaagc aagaggcgat ggccgggtcc agcgaccatg
      541 cggacgaggt gcggtaccac gaggccgacg aacaggatga tgcccgacac ggcgaccgct
      601 gacgcggtga gcaccgcgac gatggtgagc atcgccaggc gcagccggtc gacgtcgacg
      661 ccgacgtgca cggccgctcg atcacccaac gcgagcaggt cgagcgaacg ggcgcgacgt
      721 gtcgccagca cgattcctgc caccgcgacc ggcgcgacca ccgcgacctt cggccaggtc
      781 gcttgggcga cgctcccgag gctccagaac gtgatgctgc gcagctccgc atcgtcggcg
      841 aagaacgtga gcagtccgat gaccgcgccg gtgaacgcgt tgaccgcaat gccggtgagg
      901 atgagcgtga cgacctcggt gcgaccgtcc gatcgcgagg ccgcgtacac caccgcgacg
      961 gtgaccagtc cgcccacgaa cgcaaccgcg gtgaggctcc acgtgccgag cgcggcgaac
     1021 cccaacgcga tacccgcgac ggcgccgagc gccgcgcccg acgagacacc gacgatgccc
     1081 ggctcggcga gcggattgcc gaacgttcct tgcatcgacg cgcccgcgca gcccagcgcc
     1141 gcgccgacca ccacggccag cgcgatgcgc gggaaccgga tgtcccacag caccgattcg
     1201 gcggtcgggt cgggctgtgg accgaacggc aggccgaccc ggtgcgcgat cgaaccgagg
     1261 atgtcggtga tcgggatcga gaacgcgccg accgccgcgg cgaccacgca accgccgacc
     1321 agcgcgacga ccagtccgcc gatcagcacc gtgggggaca cccgccgcgg tgcggcgacg
     1381 acgtcttcga ggtcagccgg cgcggccatg gatctcgccg atcagcgtgg cgagggcggc
     1441 gggggtgcgg gcaccgaagc tgtacaacac gccgtcttcc tgggtgatca cacggcgctc
     1501 ccggcccgcg ggtgtctggg cgatgcccgg gatctgcaag agaccgtcga tgcccccgac
     1561 cgattccagg ccggtcgtgg tcatgaggat cacgtcgggc gcagcttcga cgagtgcctc
     1621 gctcgtgatc ggcgtgaacg gcttgtcgag ccccatcgcg gtgccggcgt cgatcgcgcc
     1681 ggccgcctcg atcatcgagt cggcgcccga cccgtcaccg gcgatgaggt acacgcccgc
     1741 agtgccgcgc atgtagagga acgccacgcg aggcggcggt tcgtcgtggg ggatggcgcg
     1801 ttgcgcgtcg gcgatggctg cttgggtccg ttcggccagc gcggcgccct ctgtgggaac
     1861 gccgagtgcg gccgcgatcg cgtggatgcg agtcgacacg tcctcgagct gtgtcggaag
     1921 gtcgaacacg acgaccggta cgcctgccgc ccggatctga tcgagggcct cgggcggccc
     1981 ggtgtcaggt tgcgcgagca cgacggtcgc gccgaccgac agcacgccct cggctgagac
     2041 gtcgtgcgcg tgcgtgacga gcggcagatg ttcggcttcg ggaaaggttg tcgagacatc
     2101 gcgggcgacg acgtggtcgc ccagccccaa cgcgaacacg acctcggcca ggctccccga
     2161 cagcggcacg atgcggtcgg cgtcggtgac ggtgacctcg cgtccgtcgg cggacggcac
     2221 ggtaacgggc agcgcgggtg ccggatcgtc ggcaatggga cgcagcacgt acgcctcgtc
     2281 gtatcgtgcg ccgtctccgg tcgccgatcc tgcagtgccg catgccagca gcagcgagca
     2341 cgcggccagg gcaaccgcga gcgtgcgtgc gcgatgcctc atgcgcgttg ccgtcgccaa
     2401 cggtggacac cgaacgccgt gaaaccgaga acgatggcgg cgacggcgat cgacacgccg
     2461 atcaccgcgg cactacttcc gccaccaccg tcgtcgaccg acgcggcggc cagctcgccg
     2521 ccgcgcacct cgccgcctga cggcgtggtc gtcgtcgctt cggcctctgc ggcggtggtg
     2581 gtcgacgacg cgagggcgtc gtccggcgcg gcgccgtcga ccggtgcgac gccggcatcg
     2641 ggcgcggtcg ggctctccgg cgcgggtgat gcacctgagc tcgcaccggt cggggggttg
     2701 ccgctgctcg ccggtggcgg cgtggccggt gacgcgaagt gaaccgggat gacgacgtcc
     2761 tgtgcgcggt cgctcgctcg cgtgtggtcg ttgcgagtca cgatcgcgca ctgcacgacc
     2821 cggcagtcga ccgtggcgtt gagcatcgcc gacaccgcga cggtcacgtc gaacgaaccg
     2881 cccgggccat agggagtggc gacgccttgc ccgtacgggg gcgggttgga cgagatccag
     2941 tgcgacgcgc cggatccgcc ggccatgtcg acgccgccgc cgcacgggct cggtgcctga
     3001 cccggagtcg gcgccgcgca gaacgcgacg tagatgccct tgaacgtgtc gtaaccagag
     3061 ccggtcaccc gcaccatctg accggccgga tccagaccgg ttgcctgcga caccgtgagc
     3121 gtgcgcgtgc cgtcggttgc ggtctctgcg cctccgggcg acgcggggag cacgacgaga
     3181 ccgagtgcga gcaggaaggt gccggctcga cgaaccgcgt gcatcgtgct catgacacgt
     3241 actcggcgag ctgcgcgaac agcgcaccat tcagccgaaa ggcttcggac gattcgtcga
     3301 tgatgcgcgc ctgttcggct tcgtcccacg gcgcgcgatc gaggagctcg cggtacgacg
     3361 ccttgaacgc cttggtgtcg tcgatgtcgg gaaagtcgta gaaccgcgca ccttctcggc
     3421 tgttcagccc gaacgctcgc ttcactacgc gcgcaatggc ctggccaccc gacaggtcgc
     3481 ccagatagcg cgtgtagtga tgggcgacga agccaccggg ccaggtgaac gcgacgtcct
     3541 gcaggcgacc gcagtactgc tcggtggccg gaagccgctc gatgcggttg cgccattcgt
     3601 cgcccaacca gaactggagg tcttcggcca acgccggccc tcgccgcagc gcatcgcaca
     3661 cgaacggccc ggcgatcggg tcggtgcgca tggcatcgcc ggctcgctcg agctcgccgt
     3721 agacgaaacc gagctgcccg acgaacgcgg cgtacgcctc ccgctcgatc tcgccgcgca
     3781 gcaacgcccg ggcgcaggcc gagcccacg
//
LOCUS       DASUGA010000448         4873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_960841, whole genome shotgun sequence.
ACCESSION   DASUGA010000448 DASUGA010000000
VERSION     DASUGA010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960841"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VFX21_08900"
     CDS             <1..592
                     /locus_tag="VFX21_08900"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5096118.1"
                     /translation="ERDVEAAVALASTPTGGLDVLFACAGGSLHLGPITEADADAWRA
                     TIDLNIVGTMLSIKHAAAAMQRSGGGSIIGMSSIAGTNTHRLMSAYCVGKAGIEMLVK
                     VAADELGEHGIRVNAVEPGLIDTELVGFITAGGKLLADYHEQMPVARVGTVDDVASVV
                     AFLAGPESTWITGQMVHVDGGHHLRRGPNYHLLFEQ"
     gene            713..1933
                     /locus_tag="VFX21_08905"
     CDS             713..1933
                     /locus_tag="VFX21_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5096119.1"
                     /translation="MDFEETPEESAFRGEVHTWLAQHAPPKDAGIDPEHFYLSGDASK
                     AAEDAHVAACKKWQLTLFDAGWAGITWPKEWGGRGGTGWQQRIFNEEQARFHAAVGVF
                     SVGIGMAGPTIIAWGTEAQQKRSLPPMLRGDEIWCQLFSEPGAGSDLAGLRTQAVRDG
                     DEWVVNGQKVWTSGAQYSDWGILLTRSDPDVPKHRGITAFLLDMRTPGIDVRPLRQIT
                     GVAHFNEVFLTDVRIPDANRLGQAGDGWRVANTMLSNERALIGGGGRTSFGDIARLAQ
                     QCGANEDPMLRQELARCYTRLQLIKWLGWRARSRKDQGLGPEASVLKLAASRRQELDG
                     SLVLALQGTQAMLYDTDAVAKGKWQQAFLSQWSSRIGGGTEQVQRNVIGERVLGLPPD
                     IRVDKEVPFREIGT"
     gene            complement(1943..2329)
                     /locus_tag="VFX21_08910"
     CDS             complement(1943..2329)
                     /locus_tag="VFX21_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007538294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096120.1"
                     /translation="MTNTETSEATDRVARFKSDVAEMRLSDPRASRDRIWGIIGGVLM
                     ALGIGLAVAAYFQSSGASAAFNTEGPATQRDAIVIALIGVSVTVVGAALFLRFTFAQF
                     LRFWLARLIYEQQAQTDRVVEATTQR"
     gene            complement(2337..3092)
                     /locus_tag="VFX21_08915"
     CDS             complement(2337..3092)
                     /locus_tag="VFX21_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016694000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5096121.1"
                     /translation="MLELRNVRAAYGRIEVVHGVDLVVPPGEVVAILGPNGAGKSTTL
                     KVASGLLEPTAGDVFLAGRRINGATADDLARTGVCLIPEGKGIFPNLSVKEHMRMATF
                     TGHSLASIEEIAYARFPRLAERRHQIAGTLSGGEQQMLAMARGLSTDPAILLLDELSM
                     GLAPIIVEELYAAVAEVAQAGVSILVVEQFAQTVLGVANQAAIMVHGEIVAVGPPSEL
                     TEDLSNAYLGSAAAGGAGAHDNGSRAVARDSLR"
     gene            complement(3123..3875)
                     /locus_tag="VFX21_08920"
     CDS             complement(3123..3875)
                     /locus_tag="VFX21_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5096122.1"
                     /translation="MTAVHDAPAEPLLECRGITVRFGGHIALDDVDVTATPGQVTGLI
                     GPNGAGKTTLFNVICGLLTPSTGVVRLVGRDVNRLAPYKRARLGLARTFQRLELFGLL
                     TVRENVMVAADLRARYAHEEHPDVEAIIERVGLTARIDDRVDALPTGQARLVEIARAL
                     AARPRVLLLDEPASGLDDSESDQLGQLLRSLSAEGIAVLLVEHDVQLVMEVCDLIHVL
                     DFGRIIAVGDADTIRTDESVLTAYLGASLETK"
     gene            complement(3872..>4873)
                     /locus_tag="VFX21_08925"
     CDS             complement(3872..>4873)
                     /locus_tag="VFX21_08925"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5096123.1"
                     /translation="VIAAIAVTALVGAFIALPTLRLSGIYLALATGAFAVFLDRWIFK
                     LRPFDLPFVEDKVALFSTGSISVSRLSLFGYKFDTADRQLMLCATTFAIVTIIVAWLR
                     RSPMGRRLLAMKDSPAACATIGMNLTATKLTVFMISAGIAGLGGALLGGQQQTTNPSD
                     WQFANGLPVFMVGVVGGIARVGGALFAGMSLVVLTASPAFPLLRGIDWFPKFSAATPG
                     FMGVGLGRNPNGAVSDMREPFEPVVKRWWLIGGLIATCAAMYIAVLVFDIGAWWLILG
                     VIVALLVFGQIAQVLVRPPKEATEAEVPLEWVGIDRPFRPEDVAALDRELGLVEVPT"
BASE COUNT          822 a         1653 c         1699 g          699 t
ORIGIN      
        1 cgagcgcgat gtcgaggccg cagtcgcgct ggcctcgacg ccgacgggtg ggctcgacgt
       61 gctgttcgcg tgtgcgggcg gctcgttgca tctcgggccg atcaccgagg ccgatgccga
      121 tgcctggcgg gcgacgatcg acctgaacat cgtcggcacc atgctgtcga tcaagcacgc
      181 ggccgccgcg atgcaacgca gtggtggcgg gtcgatcatc ggcatgtcgt cgatcgcggg
      241 cacgaacacg caccgcctca tgagcgcgta ctgcgtcggc aaggccggta tcgagatgct
      301 cgtgaaggtc gcggccgacg agctcgggga gcacggcatt cgcgtcaacg cggtggagcc
      361 cgggctgatc gacaccgagc tggtcgggtt catcaccgcg ggcggcaagc tgctcgcgga
      421 ctaccacgag cagatgccgg ttgcgcgcgt cggcaccgtc gacgacgtcg cgtcggtcgt
      481 cgcgttcctc gcgggcccgg aatcgacgtg gatcacgggc cagatggtgc acgtcgacgg
      541 cggccaccac ttgcgccgcg gccccaacta ccacctcctc tttgaacagt gaagcgacgc
      601 gcggaagtca cacggcgcgg tgccggagct tgcggaggca ctcgccggag tcctgaggcg
      661 ggccaacaat ccgggctgtc tcacgcgagc acgtcgaagg cgacatagaa acatggattt
      721 tgaagagacg cctgaagaga gcgcgttccg cggcgaagtg catacgtggt tggcgcaaca
      781 tgcgccgccg aaggacgcgg gtatcgaccc ggagcacttc tatctctccg gcgacgcgag
      841 caaggccgcg gaagacgcgc acgtcgcggc ctgcaagaag tggcagctca ccctgttcga
      901 cgcgggctgg gccggcatca cgtggccgaa ggagtggggc ggacgcggcg gcaccggttg
      961 gcagcagcgc atcttcaacg aagagcaagc gcgcttccac gccgcggtcg gcgtgttctc
     1021 ggtcggcatc ggcatggccg gtccgacgat catcgcgtgg ggcaccgagg cgcagcagaa
     1081 gcgctccctg ccgccgatgc tgcgcggcga cgagatctgg tgccagctgt tctccgaacc
     1141 gggtgccggc tccgacctcg ccggactccg cacgcaagcg gtacgcgacg gcgacgagtg
     1201 ggtcgtgaac ggccagaagg tctggacctc gggcgcgcag tacagcgact gggggatcct
     1261 gctcacccgc tccgaccccg acgtgcccaa gcaccgcggc atcaccgcgt tcctcctcga
     1321 catgcgcacg ccggggatcg acgtccggcc gctgcgccag atcaccggcg tcgcgcactt
     1381 caacgaggtg ttcctcaccg acgtccgcat tcccgacgcg aaccgcctcg gccaagcggg
     1441 cgacgggtgg cgggtcgcga acacgatgct gtcgaacgag cgcgcactca tcggcggtgg
     1501 aggccgcacg agcttcggcg acatcgcccg cctcgcgcag cagtgcggcg cgaacgagga
     1561 tccgatgctc cgccaggagc tcgcgcgttg ctatacgcgg ctgcagctca tcaagtggct
     1621 cggctggcgg gcccgctcac gcaaggacca ggggctcggc ccggaagcgt cggtgctgaa
     1681 gctcgcggcg tcgcgccgcc aggagctgga cggcagcctc gtgctcgcgc tgcagggaac
     1741 gcaggcgatg ctctatgaca ccgacgcggt cgcgaagggt aagtggcagc aggcgttctt
     1801 gtcgcagtgg agcagccgga tcggcggcgg caccgaacag gtgcagcgca acgtcatcgg
     1861 cgaacgcgtg ctcgggctcc cgcccgacat ccgcgtcgac aaagaagtgc cgttccgcga
     1921 gatcgggacg tagcgccgcg attcaacgct gtgtcgtcgc ctcgacgacg cggtcggtct
     1981 gcgcctgctg ctcgtagatg agacgggcga gccagaaccg caggaactgc gcgaacgtga
     2041 agcgcaggaa gagcgcggcg ccgacgacgg tgaccgacac gccgatgagc gcgatcacga
     2101 tcgcgtcgcg ctgggttgcg ggaccctcgg tgttgaacgc ggcgctcgcc ccgctcgact
     2161 ggaagtatgc ggcgacggcc aagccgatgc cgagcgccat gaggacgccg ccgatgatgc
     2221 cccaaatgcg gtcgcgtgac gcgcgtgggt cgctgagacg catctcggcg acgtcgctct
     2281 tgaaacgggc gacccgatcc gtggcttcgg aagtctcggt gttggtcatg atccccctac
     2341 cgtaacgagt cccgcgccac cgcgcggctt ccgttgtcgt gcgcaccggc accgccggcg
     2401 gccgcgctgc ccaagtaggc gttcgagagg tcttcggtca actcgctcgg cggcccgacc
     2461 gcgacgatct cgccgtgcac catgatcgcg gcctggttcg cgacgcccaa caccgtctgc
     2521 gcgaattgct cgacgacgag gatcgacacg ccggcttgcg cgacctcggc gaccgcggcg
     2581 tacagctcct cgacgatgat cggcgccaga cccatcgaca gctcgtcgag caggaggatc
     2641 gcgggatcgg tcgagaggcc gcgcgccatc gcgagcattt gctgctcgcc accggacagc
     2701 gtgcccgcga tctggtggcg ccgttccgcg agtcgcggaa aacgcgcgta tgcaatctct
     2761 tcgatgctgg cgagcgagtg gcccgtgaac gtcgccatgc gcatgtgctc cttcaccgac
     2821 aggttcggga agatgccctt gccttcgggg atcaggcaca cgccggtgcg cgcgagatcg
     2881 tcggccgtgg cgccgttgat gcggcggccc gcgaggaaca cgtcgcccgc ggtgggttcg
     2941 agcagtcccg aggcgacttt cagcgtcgtc gacttgcctg caccgttcgg gccgaggatc
     3001 gcgacgactt cgccgggcgg cacgacgagg tcgacgccgt gcacgacctc gatgcgcccg
     3061 tacgcggcgc gcacgttgcg cagctcgagc atcgggactt cttcgtcgtc actcactggc
     3121 gctcacttcg tttccaggct cgcgccgagg taggcggtga ggacggactc gtcggtgcgg
     3181 atcgtgtccg cgtcaccgac cgcgatgatg cggccgaagt cgagcacgtg gatgaggtcg
     3241 catacttcca tcacgagctg cacgtcgtgc tcgacgagca acacggcgat gccttcggcc
     3301 gagagcgacc gcaacaactg accgagctga tcggactcgc tgtcgtcgag gcccgacgcg
     3361 ggctcgtcga gcagcagcac gcgtggccgc gccgcgaggg cacgggcgat ctcgacgagg
     3421 cgggcctggc ccgtcggcaa cgcgtcgacc cggtcgtcga tgcgggccgt gagcccgacg
     3481 cgctcgatga tcgcttcgac gtcggggtgc tcctcgtgcg catagcgagc ccgcaggtcg
     3541 gcggcgacca tcacgttctc gcgcacggtc aacaagccga acagttcgag gcgctggaac
     3601 gtccgcgcga gcccgaggcg cgcgcgtttg tagggagcga gacggttcac gtcgcggccg
     3661 acgagccgca ccacacccgt cgagggcgtc agcaacccgc agatcacgtt gaacaacgtc
     3721 gtcttgcccg cgccgttcgg gccgatcaac ccggtcacct gtccgggtgt cgcggtcacg
     3781 tcgacgtcgt cgagcgcgat gtggccgccg aagcggacgg tgatgccgcg gcactccagg
     3841 agcggttcgg cgggcgcgtc gtgcaccgcg gtcacgtcgg cacctcgacc aggccgagct
     3901 cgcggtcgag cgcggcgacg tcctcgggcc ggaacggccg gtcgatgccg acccactcga
     3961 gcgggacctc ggcttcggtc gcctccttcg gcggccgcac gagcacctgc gcgatctggc
     4021 cgaagacgag caacgcgacg atgacgccga ggatcaacca ccacgcgccg atgtcgaaca
     4081 cgagcacggc gatgtacatc gccgcgcagg tcgcgatcag gccgccgatc agccaccagc
     4141 gcttcaccac cggctcgaac ggctcgcgca tgtcgctgac cgcgccgttc gggttgcggc
     4201 cgaggccgac acccatgaag cccggcgtcg ccgcggagaa cttcgggaac cagtcgatgc
     4261 cacgcaacag cgggaacgcg gggctcgccg tcagcacgac gaggctcatg ccggcgaaca
     4321 gcgcaccacc gactcgcgcg ataccgccga cgacgccgac catgaacacc ggcagcccgt
     4381 tcgcgaactg ccagtcgctc gggttcgtcg tctgttgctg cccgccgagc aacgcgccgc
     4441 cgaggcccgc aatgccggca gagatcatga acaccgtcag cttcgtcgcg gtgaggttca
     4501 tgccgatcgt ggcgcacgcg gccgggctgt ccttcatcgc cagcaaccgg cggcccatcg
     4561 gggagcggcg caaccacgcg acgatgatgg tcacgatcgc gaacgtcgtc gcgcacaaca
     4621 tgagctgccg gtcggcggta tcgaacttgt aaccgaacag gctgaggcgc gacacgctga
     4681 tcgagccggt gctgaacagc gcgaccttgt cctcgacgaa cggcaagtcg aacggtctga
     4741 gcttgaagat ccatcgatcg aggaacaccg cgaacgcgcc ggtcgccaac gcgagataga
     4801 tgccggacag gcgcagtgtg ggcaacgcga tgaacgcacc gacgagcgcg gtgaccgcga
     4861 tcgccgcgat gac
//
LOCUS       DASUGA010000449         2778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_719848, whole genome shotgun sequence.
ACCESSION   DASUGA010000449 DASUGA010000000
VERSION     DASUGA010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719848"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /locus_tag="VFX21_08930"
     CDS             <1..1432
                     /locus_tag="VFX21_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5096124.1"
                     /translation="TVAASLTLLPALLGFAGDNIERTKWRGLVAAGFVAVGLAGAGLK
                     VPALAGIGFLFAAITIIAGFVVPVLKREVERKPPKPRRETIAYRWSRVIQHRPWTATI
                     AAAAVLLILAVPFLQLRLGFSDESNFADNTTTKKAYNLIVDGFGKGSTGPIYLVASID
                     NPEQMGALAAINDAVQSDNDVEGVLGPQPNDPDNPTAVRWQIQPVGGPQDESTTHLVH
                     RLRDDVLPPIEEAAGADVLVTGAVAANVDMSAYMGERMPIFFGAVLTLSFVLLMIVFR
                     SLLVPLKAVIMNLLSIAAAYGIIIALFQWGWGSDITGVQPGPIETWVPMMLFAVVFGL
                     SMDYEVFLLSRIREEWVRTGDSKQSVADGLAATAKVITAAAAIMVVVFGSFMLEHERV
                     MKMMGIGLATAILLDATIVRMVLVPGTMELLGDKNWWLPRWLDRILPDLDVEGHAPPP
                     DDDAGEPGDGGDEPAGEPEREPVPAG"
     gene            complement(1447..2751)
                     /locus_tag="VFX21_08935"
     CDS             complement(1447..2751)
                     /locus_tag="VFX21_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020516744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX5096125.1"
                     /translation="MAVDELEPATTAPRRLNVRQAAFIGVGAMVGAGIFSLLGAAGEV
                     AGAAVWISFLLAGAIAMLQGYSFAKFGARYPTAGGLLEYVRRGFGDGHFMGVIAWLII
                     ATNAIITAMVAVSFGSYAMEAVADGNDALIKVFAVVVIAAMTMLNVVGSTAVARVQTV
                     VVVVVIGILTVFAVVTLANADFDLLAFSGYPPLKDVVSSVALTFFAFLGFGVVTFTAK
                     DLRNPKRELPRAMFLALGIATVIYLAVSLGVFGTLTVGEVIASGGTALAVAAEPTLGR
                     AGYWLMSVTGLFATAGATNAGLYPAGGLCEEMAAIGQFPPPMGRRLAGKVPIGLAFTA
                     VAAATLAVFFDLNAIASIGSAIALMVFGLVSVGHLRVRSDTGANAAVLVLAVASTAIV
                     LVTFAFTTLVDEPATAVTIVAILIASVIVDWQWKRTRAAAAA"
BASE COUNT          488 a          965 c          906 g          419 t
ORIGIN      
        1 caccgttgcc gcttcgttga cgctgctccc cgcgctgctc gggttcgcgg gcgacaacat
       61 cgagcgcacc aagtggcggg gcctcgttgc cgcggggttc gtcgcggtcg ggctcgcggg
      121 cgccggactc aaggtccctg ccctcgcggg catcggcttc ctgttcgccg caatcacgat
      181 catcgccggg ttcgtcgtcc cggtcttgaa gcgcgaggtc gagcgcaagc caccgaagcc
      241 gcgccgggag accatcgcgt accgctggag tcgcgtcatc cagcaccgcc cgtggaccgc
      301 gacgatcgcg gctgcggcgg tgttgctcat cctcgccgtc ccgttcctgc agttgcggct
      361 cgggttctcc gacgagagca acttcgcgga caacaccacg acgaagaagg cgtacaacct
      421 gatcgtcgac ggcttcggga agggctcgac gggcccgatc tatctcgtcg cgtcgatcga
      481 caaccccgag cagatgggtg cgctcgccgc gatcaacgac gcagtacaga gcgacaacga
      541 tgtcgaaggc gtcctcgggc cgcaaccgaa cgatcccgac aaccccaccg ccgtgcgctg
      601 gcagattcaa cccgtcggtg gaccgcaaga cgagtccacg acacatctcg tccaccgctt
      661 gcgcgacgac gtgctgcctc cgatcgagga ggccgcgggc gccgacgtcc tcgtcaccgg
      721 cgcggtcgcg gccaacgtcg acatgtcggc gtacatgggt gagcgcatgc cgatcttctt
      781 cggcgcggtc ctcacgttgt ccttcgtgtt gctgatgatc gtgttccggt cgttgctcgt
      841 gccgttgaag gccgtgatca tgaacctgct gtcgatcgcc gcggcgtacg gcatcatcat
      901 cgccctcttc caatggggtt ggggcagtga catcaccggt gtccaaccgg gacccatcga
      961 gacatgggtg ccgatgatgc tgttcgcggt cgtgttcgga ttgtcgatgg actacgaggt
     1021 gttcctcttg tcccgcatcc gcgaggaatg ggtccgcacc ggcgacagca agcagtcggt
     1081 ggccgacggc ctcgccgcga ccgcgaaggt catcacggcc gcggccgcga tcatggtcgt
     1141 cgtcttcggc agcttcatgc tcgagcacga acgcgtgatg aagatgatgg gcatcgggct
     1201 cgcgaccgcg atcctgctcg atgccaccat cgtgcgcatg gtgctcgtgc cgggaacgat
     1261 ggaactgctc ggcgacaaga actggtggct cccgcgctgg ctcgaccgga tcctgcccga
     1321 cctcgacgtc gaagggcacg caccgccgcc cgacgacgac gcaggcgaac cgggagacgg
     1381 gggcgacgaa ccggcgggcg agcccgagcg cgaaccagtc cccgcgggat agccgtcagt
     1441 ggcggctcag gccgcggcgg cagcgcgtgt gcgcttccac tgccagtcga cgatcacgct
     1501 ggcaatgagg atggcaacga tcgtcaccgc ggtcgccggt tcgtcgacca acgtcgtgaa
     1561 tgcgaacgtg accaaaacga tcgcagtcga cgcaaccgcg aggaccagca ccgcggcatt
     1621 cgcgcccgtg tccgatcgca cgcgcaggtg cccgaccgag accagaccga agaccatcag
     1681 cgcgatcgcg ctgccgatcg acgcgatcgc gttcaggtcg aagaagactg caagcgtcgc
     1741 cgccgcgacc gcggtgaacg cgaggcctat cggcaccttc ccggccagcc gccgacccat
     1801 cggcggcggg aactgaccga tcgccgccat ctcctcgcac agcccaccgg ccggatacag
     1861 accggcgttg gtcgcaccgg cggtcgcgaa gagcccggtc acgctcatca gccagtagcc
     1921 ggcccgaccc agggtcggct ccgccgcgac ggccagcgcg gtaccaccgg acgcgatcac
     1981 ctcgccgacc gtgagggtgc cgaagacacc cagtgacacc gcgaggtaga tcacggtcgc
     2041 gatcccgagc gcgaggaaca tcgcgcgtgg cagctcgcgt ttcgggttgc ggagatcctt
     2101 cgccgtgaac gtcacgaccc cgaagcctag gaaggcgaag aaggtgaggg cgacactcga
     2161 aacgacgtct ttcaacggcg ggtagcccga gaacgcgagc aggtcgaagt cggcattcgc
     2221 gagcgtcacg accgcgaaca ccgtgaggat cccgatgacg acgaccacga caaccgtctg
     2281 cacccgcgcc actgcggtcg atcccacgac gttcagcatt gtcatggccg cgatcacgac
     2341 taccgcaaag accttgatga gcgcgtcgtt gccatcggca actgcttcca tcgcgtagct
     2401 gccgaacgac acggcgacca tcgcggtgat gatcgcgttg gtcgcgatga tcagccacgc
     2461 gatgacgccc atgaagtggc cgtcgccgaa gccgcgccgc acgtactcga gcagcccacc
     2521 cgctgtcggg taccgggcgc cgaacttcgc gaacgagtag ccctggagca tcgcgatcgc
     2581 gcccgcgagc aggaacgaga tccataccgc ggcgccggcg acctcgccgg ccgcgccgag
     2641 cagcgagaag atcccggcgc cgaccatcgc gccgacgccg atgaacgcgg cctggcggac
     2701 gttcaggcgg cgcggcgccg tcgttgcggg ctcgagttca tctactgcca tccgtcatcc
     2761 ctcgccgggc cgtctccg
//
LOCUS       DASUGA010000450         4611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_283268, whole genome shotgun sequence.
ACCESSION   DASUGA010000450 DASUGA010000000
VERSION     DASUGA010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283268"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VFX21_08940"
     CDS             <1..550
                     /locus_tag="VFX21_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096126.1"
                     /translation="AAAEARIPEVARVAAEREAADARDAHQRYLDDLAAAERARDDDL
                     KATRSASEREADIARAAVLEQVAAERAELEAALEERAGAHEALEAATSAADAASIVVA
                     EKRADLAQYAAAEAERLAAEAREAARRAEEEAKAALRRAQELTDAARRRSDQAQAALN
                     EAMAAQHVLATDNVVDLSAPDV"
     gene            complement(570..2036)
                     /locus_tag="VFX21_08945"
     CDS             complement(570..2036)
                     /locus_tag="VFX21_08945"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX5096127.1"
                     /translation="MASVRRVLDTDPVLHLQQYLASGGGAGLRRARDLGADATIAEVV
                     AAGVRGRGGAGFPTGVKWQTVAANVSPLEPTTVVVNAAEGEPGSFKDRALIARNPYRV
                     LEGALIAAYAVGADEVIVGMKRTFEAHIARMNDAIDEVLNADLAPGISIAIFEGPPEY
                     LLGEETGLLESIDGRMPFPRIAPPYRRGADEIVEHRSDVDDESASAAHVEMAGPSDEA
                     LAPPTLVDNVETLAHVALVLEHGADWFRELGTDQSPGTFVCTVSGDVRHAGVGEFPMG
                     TPLREVIETLGGGSREERTIKAVLQGVSAPVLTADDLDTPLSWEGMVAAGSGLGAAAF
                     IVFDDLADMAAVAAGVARFLAVESCGQCTPCKLDGITIADALDTIRRSEGTAHEAEIV
                     AERLVTVADSARCSLATQHQVLVQSIVDRFASEFDAHLHGGATSRFEIAPLVDLVDGN
                     AVTDPNQATKQPDWSHDEIWSGKSPADALDDHRAHQHL"
     gene            2108..3343
                     /locus_tag="VFX21_08950"
     CDS             2108..3343
                     /locus_tag="VFX21_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011595113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5096128.1"
                     /translation="MQLSDINLLDRDAFTKGVPHDWFTFLRREAPVYKHPEPNGPGFW
                     VITKYDDVVAVGRDGTTFSSHQNRGGVVLLEGAPEDNTFEAGGNLMLTMDAPEHTRHR
                     KLVNRGFTPKRMRDLEPHLRELTSQIVDEVIEQGGCDFVVEVAAELPLQVIAEMIGVP
                     QEDRHKVFEWSNRMIGSEDPEYSVSVEEVQGAQVEMFMYANQLAAERRANPRDDIMTT
                     LLNADVDGDQLSEMDFNLFFLLLAVAGNETTRNSISHGIRAFCDYPDQYQKLVADPSL
                     AAPATEEIVRWASPVMYFRRNVTRDTEIRGQEIKEGDKVSIWYISANRDEDIFGDPFT
                     FDITRSPNEHVGFGGGGPHHCLGANLARMEIRLLLEEMARRIPSIERVGEAQPLRSNF
                     IAGIKHMPVKYPPGTREAS"
     gene            3412..>4611
                     /locus_tag="VFX21_08955"
     CDS             3412..>4611
                     /locus_tag="VFX21_08955"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5096129.1"
                     /translation="MREVAFTSKRWLVQGISSSLGWTLAKIAIPLCALQAINDGIDPY
                     RPSALVKWAVAIVVLTAITGTLTYTRRTAAFAISLRAEAELRRRLFNHLQRLHFAYHD
                     RAQIGELMARVSTDAKQVQMLLVFIPIAGANFVMVVGVTVVLFTLDAKLAAVAMASLP
                     FLLLFAIRLSTLLHPVAMGLQERLAGVSHVVEESLSGIRVVKGFGAEDLQNEHLAVAA
                     DGVYERGIALARLRARYNPLLEVLPTLGLVGILYVGGHEVIDGNLSVAALITFNFYIL
                     QLVFPLRMTSFMVAQISRASASATRVYEILATDPEIVERPHSEPLPEGRGALEFRDVT
                     FGYGAALPVLRDFTLSIAPGESVALVGGTGSGKSTVARLVPRFYDVAAGEVVLDGADV
                     RTVSLSDL"
BASE COUNT          736 a         1574 c         1613 g          688 t
ORIGIN      
        1 cgcggcggcc gaggcccgga tcccggaggt cgcgcgagtt gccgccgaac gcgaagccgc
       61 ggacgcgcgt gacgcgcacc agcgatacct cgacgacctc gctgccgcgg aacgagcgcg
      121 cgacgacgac ctcaaggcaa cgcgttcggc gagcgaacga gaggccgaca tcgcccgcgc
      181 ggccgtcctc gaacaggtcg cggccgaacg ggccgagctg gaggccgcgc tcgaagaacg
      241 cgcgggcgct cacgaggcgt tggaggccgc gacgagcgcg gccgacgcgg cgtcgatcgt
      301 ggtggccgag aagcgggccg acctcgcgca gtacgcggcg gccgaggccg agcggctcgc
      361 cgccgaagca cgcgaggccg cccgccgggc cgaagaggag gccaaggcgg cgctgcgccg
      421 ggcccaggag ctgaccgatg ccgcccgccg gcgctccgat caggcccagg ccgcactgaa
      481 cgaggccatg gccgcgcagc acgtgctcgc caccgacaac gtcgtcgacc tgagtgcacc
      541 ggacgtatag cgcgcggccg gtacgcgtac tacaagtgct ggtgggcgcg gtggtcgtcg
      601 agtgcgtcgg cggggctctt gcccgaccag atctcgtcgt gtgaccagtc gggttgcttg
      661 gttgcctgat tcgggtcggt gacggcgttg ccgtcgacga gatcgacgag cggcgcgatc
      721 tcgaagcgcg acgtcgcgcc gccgtgcagg tgcgcgtcga actcggacgc gaaccggtcg
      781 acgatgctct gcacgagcac ctgatgctgc gtcgccagtg agcagcgtgc gctgtcggcg
      841 acggtgacga gccgctcggc gacgatctcc gcttcgtgcg cggttccctc cgagcggcga
      901 atggtgtcga gcgcgtcggc gatcgtgatg ccgtcgagct tgcagggtgt gcactgacca
      961 cacgactcga ccgcgaggaa ccgcgcgacg cccgccgcga cggcggccat gtcggcgagg
     1021 tcgtcgaaca cgatgaacgc ggccgcaccg aggcccgagc ctgcggcgac catgccctcc
     1081 cagctcagcg gggtgtcgag gtcgtcggcg gtcaggaccg gcgcggaaac gccttgcagg
     1141 acggccttga tcgttcgttc ctcgcgcgag ccgcccccga gggtctcgat cacttcgcgc
     1201 agcggtgtgc ccatcgggaa ctcgccgacg cccgcgtgcc gaacgtcgcc gctcaccgtg
     1261 cacacgaacg tgccgggcga ctggtcggta ccgagctcac ggaaccagtc ggcgccgtgt
     1321 tcgagcacga gcgcaacgtg cgcgagggtt tcgacgttgt cgacgagcgt cggcggcgcc
     1381 aacgcctcgt cgctcggtcc cgccatctcg acgtgcgcgg ccgacgccga ctcgtcgtcg
     1441 acgtcgctgc ggtgttcgac gatctcgtcg gcgccgcggc ggtacggcgg tgcgatgcgc
     1501 gggaacggca tgcgcccgtc gatcgattcg agcaacccgg tctcctcgcc gagcaggtac
     1561 tcgggcggac cctcgaagat cgcgatcgag atgccgggcg cgagatcggc gttcagcact
     1621 tcgtcgatcg cgtcattcat gcgcgcgatg tgcgcctcga acgtgcgctt catgccgacg
     1681 atcacctcgt cggcgccgac ggcgtaggcg gcgatgagcg cgccttcgag cacgcggtac
     1741 gggttgcggg cgatgagcgc gcggtccttg aacgagccgg gctcgccttc ggccgcattg
     1801 acgacgacgg tcgtgggttc gagcggggac acgttcgccg cgactgtctg ccacttgacg
     1861 cccgtcggga agccggcacc gccgcggccc cggacgccgg cggcgacgac ttcggcgatc
     1921 gtcgcgtccg caccgaggtc acgggcccgg cgcagccccg cgccgccacc ggacgcgagg
     1981 tattgctgca ggtggaggac cgggtcagta tcgaggacgc gacgaacgga ggccatgggg
     2041 acgtccttcc cagatccggg ccgcacgaaa gtagaacggg ttccagattc gcgaatacgc
     2101 tcgtgacatg cagctgtcgg acatcaacct gctggaccgc gacgccttca ccaagggcgt
     2161 cccgcacgac tggttcacgt tcctgcggcg ggaggccccg gtctacaagc accccgaacc
     2221 gaacggcccc gggttctggg tgatcaccaa gtacgacgac gtcgtcgcag tcggccgcga
     2281 cggcacgacg ttctccagcc accagaaccg tggtggtgtc gtactcctcg aaggtgcgcc
     2341 ggaagacaac acgttcgagg ccggcggcaa cctcatgctc acgatggacg cgcccgagca
     2401 cacgcggcac cgcaagctcg tcaaccgcgg gttcacgccg aagcggatgc gcgacctcga
     2461 accgcacttg cgcgagctga cgtcgcagat cgtcgacgag gtgatcgagc agggtggatg
     2521 tgacttcgtg gtggaagtcg cggcggagct gccgctgcag gtgatcgcgg agatgatcgg
     2581 tgtgccgcag gaagaccggc acaaggtgtt cgagtggtcg aaccgcatga tcggcagcga
     2641 agatcccgaa tacagcgtct ccgtggagga ggtgcagggc gcgcaggtcg agatgttcat
     2701 gtacgcgaac cagctcgcgg ccgagcggcg cgccaacccg cgcgacgaca tcatgacgac
     2761 gttgctcaac gccgacgtcg acggcgacca gttgagcgag atggacttca acctgttctt
     2821 cttgctgctc gcagtcgccg gcaacgagac cacgcgcaac tcgatctcgc acggcatccg
     2881 cgcgttctgc gactaccccg accagtacca gaagctcgtc gccgacccgt cgctggccgc
     2941 acccgcgacg gaggagatcg tgcgctgggc gtcgccggtc atgtacttcc ggcgcaacgt
     3001 cacgcgtgac accgagatcc gcgggcagga gatcaaggaa ggcgacaagg tcagcatctg
     3061 gtacatctct gcgaaccgcg acgaagacat cttcggcgac ccgttcacgt tcgacatcac
     3121 gcgcagcccg aacgagcacg ttggcttcgg cggcggcggc ccgcaccact gcctcggcgc
     3181 gaacctggcc cgcatggaga tccggctgct gctcgaagag atggcgcgcc gcattccgtc
     3241 gatcgaacgg gtcggcgagg cgcagccgct gcgctcgaac ttcatcgccg gcatcaagca
     3301 catgccggtg aagtacccgc ccggcacccg cgaagcgagc tgacagcgac tcgcactgag
     3361 cccaaacgcg cgaaggcgcc tgcgcatctg cggcggggcg ggtggcgcct catgcgggag
     3421 gtcgccttca cgagcaaacg gtggctcgta cagggcatca gctcgtcgct cggttggacg
     3481 ctggccaaga tcgcgatacc gctctgcgca ctgcaggcga tcaacgacgg catcgacccg
     3541 tatcgcccga gtgcgctggt caagtgggcg gtcgcgatcg tcgtgctcac ggccatcacc
     3601 ggcacgctga cctacacccg gcgcaccgcc gcgttcgcga tctcgctgcg ggccgaggcc
     3661 gagttgcgcc ggcgcctgtt caaccatctg caacgcctgc acttcgccta ccacgaccgg
     3721 gcccagatcg gcgagctgat ggcccgggtc agcaccgacg ccaaacaggt gcagatgctg
     3781 ctcgtgttca tcccgatcgc gggcgccaac ttcgtgatgg tcgtcggcgt gaccgtcgtg
     3841 ctgttcacgc tcgacgcgaa gctcgccgcg gtggcgatgg cgtcgctgcc gttcttgctg
     3901 ctcttcgcga tccggctctc gacgttgctg cacccggtcg cgatgggctt gcaggaacga
     3961 ctggccggcg tgtcgcacgt cgtcgaggag agcctgtcgg gcatccgcgt ggtgaagggc
     4021 ttcggggccg aggacttgca gaacgagcat ctcgcggtcg cggccgacgg cgtctacgaa
     4081 cgcggcatcg cgctggcgcg gttgcgagcg aggtacaacc cgctgctgga agtgctcccg
     4141 acgctcggtc tggtcggcat cctctacgtc ggcgggcacg aggtcatcga cggcaacctg
     4201 agcgtcgccg cgctgatcac gttcaacttc tacatcttgc agctcgtgtt cccgctgcgg
     4261 atgacgtcgt tcatggtcgc ccagatctcg cgcgcgtcgg cgtccgcgac ccgcgtgtac
     4321 gagatcctcg ccaccgatcc cgagatcgtc gaacgcccgc actccgagcc gctgcccgaa
     4381 gggcgcggcg cgctcgagtt ccgcgacgtg acgttcggat acggcgccgc cctgccggtg
     4441 ctgcgggatt tcacgctgag catcgccccg ggcgagtcag tcgccctggt cggcggcacc
     4501 ggttcgggca aatcgaccgt cgcgcggctg gtgccgcgct tctacgacgt cgccgcgggc
     4561 gaggtcgtgc tcgacggggc cgatgtgcgc acggtgagcc tctccgacct g
//
LOCUS       DASUGA010000451         2082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_586100, whole genome shotgun sequence.
ACCESSION   DASUGA010000451 DASUGA010000000
VERSION     DASUGA010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586100"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1650)
                     /locus_tag="VFX21_08960"
     CDS             complement(<1..1650)
                     /locus_tag="VFX21_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX5096130.1"
                     /translation="MLAVGALATTAMAQFTLVTPNGFASTPGSTNNSFPWNRGTASMR
                     IQFLIDSTHFTAQGVVSPIIISKLRYRPYAQTTATVASWAGGSWPNVRIDMATSPLDW
                     ASASGTFANNLGPDLTTVLNGPVTVQGGSTTGVGVVVPWHIDIPLTTNFVYDPTSGND
                     LTVDIYLDGTGWTGTSRSADHHAVVGQVLGSRVYNTTGITATTGTVALNYGAVTEFTY
                     IPANGLFAGFGADVTGGASPLSVNFTDQSFSSAPGGITSYAWDFNGDSVIDSTAQNPS
                     FVYTSCGNYNVSLTVTDGVNPPSTMTKPAFIKTDQITADFTSQVVGPLTVQFTDNSNM
                     AATSWDWDLDGDGLTDSTLQNPAFVYPSLTATNVTLKVTRLCSPISQVTKLVIPAQQL
                     TTNLTGATTTGTPTTLYFNLDVLNPLGASISSFDSITANVSTAFTVDVYLKQGTHVGS
                     EANAAAWTKVGTASGTSNPVAAQPSNSAFPLPLHIPQGSYGVALRFIGITPRYLSGAN
                     TYANGDLSLTTGAAQSTSAGPFTGGVLTPRTWSGTLYYGTTN"
BASE COUNT          337 a          615 c          771 g          359 t
ORIGIN      
        1 gttggtcgtg ccgtagtaga gcgtgccgct ccacgtgcgc ggggtgagca cgccgccggt
       61 gaacggacct gccgaggtgg actgagcagc gcccgtggtg agcgacaggt cgccgttggc
      121 gtaggtgttc gcgccggaca agtagcgcgg cgtgatgccg atgaagcgca gcgcgactcc
      181 gtaggagccc tgcgggatgt gcagcggcag cgggaacgcc gagttggagg gctgtgccgc
      241 gaccgggttc gacgtgccgc tcgcggtccc gaccttcgtc cacgccgccg cgttggcctc
      301 ggagcccacg tgggtgccct gcttgaggta gacgtcgacg gtgaacgccg tgctcacgtt
      361 ggccgtgatc gagtcgaacg aggagatcga ggcgccgagc gggttgagga cgtcgaggtt
      421 gaagtagagg gtcgtgggcg tgcccgtggt cgtggcgccg gtgaggttcg tggtgagctg
      481 ctgcgctggg atgaccaact tcgtgacctg cgagatcggc gagcacaagc gtgtcacctt
      541 caacgtcacg ttggttgcgg tgaggctcgg gtagacgaag gccgggttct gcaaggtgct
      601 gtcggtgagg ccgtcgccgt ccaggtccca gtcccagctc gtcgccgcca tgttcgagtt
      661 gtcggtgaac tgcacggtca gcggcccgac gacctggctg gtgaagtcgg ccgtgatctg
      721 gtcggtcttg atgaacgcgg gtttggtcat cgtgctcggt ggattcacac cgtcggtgac
      781 cgtcagcgag acgttgtagt tgccgcaact ggtgtagacg aacgacgggt tctgggcggt
      841 cgagtcgatg accgagtcgc cgttgaaatc ccaggcgtag ctcgtgatgc caccgggcgc
      901 gctcgagaac gactggtcgg tgaagttgac cgacagcggc gatgcaccac ccgtgacgtc
      961 ggcgccgaag cccgcgaaca ggccgttggc gggaatgtag gtgaactcgg tgactgctcc
     1021 atagttgagg gcgacggtac ccgtcgtcgc cgtgatgccc gtcgtgttgt agacgcggct
     1081 gcccaagacc tggcctacga ccgcatggtg gtccgccgag cggctcgtgc cggtccaacc
     1141 cgtgccgtcg aggtagatgt cgacggtcag gtcgttgccc gacgtcgggt cgtagacgaa
     1201 gttcgtcgtc agcgggatgt cgatgtgcca cggcacgacg acgccgacgc ccgtggtcga
     1261 gcccccttgc acggtgaccg gtccgttgag cacggtcgtg aggtccgggc cgaggttgtt
     1321 cgcgaacgtc ccgctcgcag aagcccagtc gagtggactc gtcgccatgt cgatgcgcac
     1381 gttcggccac gaaccgcccg cccaggaggc caccgtggcc gtcgtctggg cataggggcg
     1441 gtaacgcaac ttcgagatga tgatcgggct gaccacgccc tgcgccgtga agtgcgtgct
     1501 gtcgatgagg aactggatgc gcatgctcgc cgtgccgcgg ttccagggga acgagttgtt
     1561 ggtgctgccc ggcgtgctcg cgaaaccgtt cggagtgacg agcgtgaact gcgccatcgc
     1621 ggtcgtggcg agggcgccga ccgcgagcat ggaaacaaga cggtgcatga tgtgctgtcc
     1681 tgaggttgtg ggtgaaacga gggctcccga gacaggagct ggagaagacg aagcctagga
     1741 agagggcaac gcattggaac cccctgtgcc tgaaactcag aactgtcgca cgccgggaat
     1801 gccctgtgcc gcatcgcgcg cgatgcgaac ccgccaatgg gagcgcgacg acgcagcgca
     1861 gccgcgtgcc gcagcacgtt ccgttcgcac cggcgcaaag caggcgaagc ggcgccggcg
     1921 tcgtcgtggg gaaccgcgtg caagcgcacg gcgacgctgg cgggctggct agcgcgactc
     1981 gaagcggccg ctgatcacga cgtcggcgta gctgccgttg ccgacttcga cgaacgtgcg
     2041 catctcttcg ccggccggga tctcgatctg gccgcgccac tc
//
LOCUS       DASUGA010000452         9287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1117009, whole genome shotgun sequence.
ACCESSION   DASUGA010000452 DASUGA010000000
VERSION     DASUGA010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 9287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9287
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117009"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2866
                     /gene="gltB"
                     /locus_tag="VFX21_08965"
     CDS             <1..2866
                     /gene="gltB"
                     /locus_tag="VFX21_08965"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEX5096131.1"
                     /translation="NEELAKLRYIDDPESGVACFSPIVVYCQYPVAEGGEGLRMAIDT
                     VRTQVSDAIANGANIIILSDRYADAENAPIPSLLITAAVHHHLVREKTRTQVGLVVET
                     GEAREVHHFALLLGYGAGCINPYLAFDTIRDLCREGVLSGLTERKAQKNYVKAAGKGI
                     LKVMSKMGISTVASYTGAQIFEAVGIRKDVVDEYFTGTASRIEGIDLDVIADEVAARH
                     RIAYPDRPEELAHRELPVGGEYQWRREGEYHLFNPDTVYKLQHSTRTGRYDIFKDYTR
                     LVDTQARRLATLRGLFQLKEGVRPPVPIDEVEPVSEIVKRFSTGAMSYGSISREAHET
                     LAIAMNRLGARSNTGEGGEDPDRFVPMANGDSKRSAIKQVASGRFGVTSEYLVNSDDL
                     QIKMAQGAKPGEGGELPGHKVWPWIAKTRHSTPGVGLISPPPHHDIYSIEDLKQLIHD
                     LKNSNPRARVHVKLVAEIGVGTVAAGVSKAHADVVLISGHDGGTGAARLTSIKHAGAP
                     WELGLAETQQTLLLNGLRDRIVVQCDGQLKTGRDVMIAALLGAEEFGFATAPLVVSGC
                     IMMRVCHLDTCPVGVATQNPELRKKFSGKPEFVVHFMEFIAEEVREYLAALGFRSLEE
                     AIGHVEVLDTQAAVDHWKARGLDLSPILAPPVNKYDQTFFCSRGQDHGLERALDVQLI
                     ADAEPALERKEPVRAAYEIHNTNRTVGTMLGSELTRRHGADGLPDGTIELTFLGSAGQ
                     SFGAFVPKGITLRLEGDANDYFGKGLSGGTLVVRPPRNAKFVAEENIIAGNVILYGAT
                     GGFTFIRGVVGERFCVRNSGATAVVEGVGDHGCEYMTGGRAVILGPTGRNFAAGMSGG
                     FAFVWDPDRSLYRRMNHDMVDIDTLDDEDADWLRGVVTMHMEETGSPVAERILSKWWR
                     NVSSFAKIFPKDYKRVLEAQRDAIERGVDVDEAIMAAARG"
     gene            2859..4319
                     /locus_tag="VFX21_08970"
     CDS             2859..4319
                     /locus_tag="VFX21_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016892636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEX5096132.1"
                     /translation="MGETTGFMKYDRELPKRRSIPVRVLDWREVYEDFPADKVRTQGA
                     RCMDCGIPFCHTGCPLGNLIPEWNDLVYRDRWQNAADRLHATNNFPEFTGRLCPAPCE
                     AACVLGINEPPVTIKQIEVEIVDRAWEEGWVTPVVPEKRTGKEVAVVGSGPAGLAAAQ
                     QLTRAGHEVVVYERADRVGGLLRYGIPEFKMEKRHLDQRVAQMEAEGTAFRTNANVGD
                     NVPVNEILGYDAVVLAGGATAWRDLPVPGREYKGVYQAMEFLPWGNRVQFGDVVEAPI
                     TAKDKRVVIIGGGDTGADCLGTSIRQGAASVHQFEILPRPPDARVDSMPWPTYPMIYR
                     VSSAHEEGGERVYAVNTECFLGDDDGNVRALRAHEVEQKIVDGRMTFEKVEGTDFELP
                     CELVLLAMGFVGPQREGLLDQLGVDLNERGNVSRDGRYKTNLDNVFVAGDMGRGQSLI
                     VWAIAEGRSCASAVDEYLMGSTLLPAPISPDAVPMR"
     gene            complement(4316..9229)
                     /locus_tag="VFX21_08975"
     CDS             complement(4316..9229)
                     /locus_tag="VFX21_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096133.1"
                     /translation="MIRTATRWALAGALVVTALAVVLVGARVPRANADGFLVGCPAGP
                     DTELGTIHFVAFQITPANPNLPPTVIVLNLPQLASALGTPAARNLDTDADGTPDLQWN
                     LELGATDLSATNLEPLWDRVGSALDASNPYTAVRVSFDRLATDLSVAPFRINGGALFD
                     LVNGGNPTTTAVNLGYDTSPTGAAPPTHLDMTVVFQDAPPAAPDSGYLGARISSQYRA
                     GGDPAAALVTPGSEPPLNTFVKVGNFNVVDEDLDLSIDWARMPAQFAFVDATTCTGPA
                     PNRTFAWDDRDEQPGVADDTVAPSDADVELTIGKAKGVAGRPELHLAGTVHAVPEEMT
                     ARLTETNIRLFHQQHPDGNGNSAPSSPAPDLDVGELVVTALSSGPGDADTRLAVNGFV
                     SDLPPVTNVDVLRDATGAFQEADVRFCDGAWASNECNDTLGTVGEIGLVASNVLPGET
                     TSLPTFALQNGPIVAYANRDIGGQLSLTQEQRFRFAARMPGVRHVHFGPPVLNTTSSA
                     FAELQLASSVPSAKVVFDSDTRHYDLDANLQALVKGARFVLNGTLTPVPAHATFGYTS
                     TNAVPFSASYQLFGAQGIFDASFTYEGNTANSLLRVAGRGLLGDATAGMPAKATMTYT
                     AAPTATGLSRLDYTASSKTRLRAGFVTSTSADRAKQRRTRVWGDVTLGTRLVAEWQRT
                     DASLRHFELRPCDVAPCTAGTVFDAVSARAVYGPPVLGEAIGILSAPALPAVQPAATY
                     PAFTSTPFGITYVQNGTDDWGARVELSKVKRVRYDYPSLTTGRKVCAETSALPKTVTD
                     KRFGTRVFVSGLVDRKGLPIDPIYVDGILNQLPTVLDARVNDEGSANNPLSIPLLWID
                     TRRCDGTTQPTGSAIEASAFEYRVLAKIGATAAHQTMDIDPLGKASIGLGKPTAATPL
                     GATVHVLSDGTADVTGVDVAARVPIPTQLVAYRPNLDVCDDSTPPNEINACQSQQMFT
                     RDARTFVSMKILSTIAEYGAADVLVRIRGHHTTDDPDSPATPNECLVGPGGKKVCDLD
                     IAGHVDHVPGMVQVLASFVTNRRAPNLDAKFEIDTLGSNPALFPTIGAVHATVLDDAS
                     PAYVGAGSSKTRTNNDDVSLTGVEHAIHAELVYQGNESMPSHHNVCPDHNYVGRGSMG
                     VEYIHAEVDVGVATSVVARARVDVFGRLEAELMTDQPVNGTVYAKAKNVGFGYKDEIL
                     TVFDTSDIDLCIDVDLPFRMTLQNTTVARLGVDQTAWALQTASSSQKITLRFGERQAD
                     GTFLSGARWFKHFAYYNPEGPGEWQYSQTGRRSVYLMPLPYWNGTEILNNFDDEWALA
                     NQINTGKMFGNDDAPTCCVAGNKTTFLLDLFWNQTIRDDMYCRDRPACVPDFAGRPGF
                     EFHKAIWQRGHLPPTGNLVMGALSNPNPAPATTGSFSLTTSGTDNTMDVETSKYAFAT
                     GTVVTGLDGTRYRIAFGPSNPDEGNEPTRMVVSVAGDYSNGVARWRREVTAYTPTLCL
                     VSFPCTVSGTMERLPSGLVLVNWNLHVHNGSFYDDESGQFIVDPSGYGGPVSTLWESM
                     TATHGQPTVVEHGFTDPGFDLYATKKVVYLGDGTIVDQGTSVGAAAPVTHTYAFPGKY
                     PGVIVYYTWLNDHWRPLYTLPFEMTVT"
BASE COUNT         1508 a         3032 c         3231 g         1516 t
ORIGIN      
        1 gaacgaagag ctcgcgaagc tgcgctatat cgacgatccc gagagcggcg tcgcctgctt
       61 ctcgccgatc gtcgtgtact gccagtaccc ggtcgccgaa ggcggcgagg gcctgcgcat
      121 ggcgatcgac accgtgcgca cgcaggtgag cgacgcgatc gcgaacggtg ccaacatcat
      181 catcctctcc gaccggtacg ccgacgcgga gaacgcgccg atcccgtcgt tgctcatcac
      241 cgccgcagtg caccaccacc tcgtgcgcga gaagacccgc acccaagtgg ggctcgtcgt
      301 cgagacgggt gaagcgcgtg aggtgcacca cttcgcgttg ttgctcgggt acggcgcggg
      361 ctgcatcaac ccgtatctcg cgttcgacac gatccgcgat ctgtgtcgcg agggtgtgct
      421 gagtgggctg accgaacgca aggcgcagaa gaactacgtc aaggccgcgg gcaagggcat
      481 cctcaaggtg atgtcgaaga tgggcatctc gacggttgcg tcgtacaccg gtgcgcagat
      541 cttcgaagct gtcggaatcc gcaaggacgt cgtcgacgaa tacttcaccg gtaccgcgtc
      601 gcgcatcgag ggcatcgacc tcgacgtcat cgccgacgaa gtcgcggcgc ggcaccgcat
      661 cgcctacccc gaccggcccg aagagctcgc gcaccgcgag ctgcccgtcg gcggcgaata
      721 ccagtggcgg cgcgaggggg agtaccacct cttcaacccc gacacggtct acaagctgca
      781 gcactcgacg cgcaccggcc gctacgacat cttcaaggac tacacgcgcc tcgtcgacac
      841 gcaggcccgc cggctcgcga cactgcgcgg cctgttccag ctgaaggaag gcgtgcggcc
      901 gccggtgccg atcgacgagg tcgaaccggt cagcgagatc gtcaagcggt tctcgaccgg
      961 cgcgatgagt tacgggtcga tctcgcgcga agcgcacgag acgctggcga tcgccatgaa
     1021 ccgactcggc gcgcgttcga acaccggtga aggcggcgag gaccccgacc ggttcgtgcc
     1081 gatggcgaac ggcgactcga agcgcagcgc gatcaaacag gtcgcgagcg ggcgcttcgg
     1141 tgtcacgagc gagtacctcg tcaacagcga cgacctgcag atcaagatgg cgcagggtgc
     1201 gaagccgggc gagggtggtg agctgccggg ccacaaggtg tggccgtgga tcgcgaagac
     1261 gcggcactcg acgccgggtg tcggcctgat ctcgccgcct ccgcaccacg acatctattc
     1321 gatcgaagac ctcaagcagc tcatccacga cttgaagaac tcgaacccgc gcgcgcgggt
     1381 gcacgtgaag ctcgtcgccg agatcggcgt cggcacggtc gcggccggtg tgtcgaaggc
     1441 gcacgcggac gtcgtgctca tctccggtca cgacggcggc acgggtgccg cgcggctcac
     1501 gagcatcaag catgccggcg cgccgtggga gctcggtctc gcggagacgc agcagacgct
     1561 gttgctgaac ggcctgcgcg accgcatcgt cgtgcagtgc gacggccagc tgaagaccgg
     1621 ccgcgacgtc atgatcgcgg cgttgctcgg cgcggaggag ttcggcttcg cgaccgcgcc
     1681 gctcgtcgtc agcggctgca tcatgatgcg cgtctgccac ctcgacacct gtccggtcgg
     1741 tgtcgcgacg cagaaccccg agctgcgcaa gaaattctcg ggcaagcccg agttcgtcgt
     1801 gcacttcatg gagttcatcg ccgaagaagt gcgggaatac ctcgccgcgc tcggtttccg
     1861 ctcgctggaa gaggcgatcg gtcatgtcga agtgctcgac acccaggctg cggtcgacca
     1921 ctggaaggcg cgcgggctcg acctgtcgcc gatcctggcg ccgccggtca acaagtacga
     1981 ccagacgttc ttctgctcac gcggccagga ccacggcctc gaacgcgcgc tcgacgtgca
     2041 gctcatcgcc gacgccgaac ccgcgctcga acgcaaggag ccggtgcgcg cggcgtacga
     2101 gatccacaac acgaaccgca ccgtcggcac gatgctcggc tccgagctga cgcgccgcca
     2161 cggcgccgac ggtctgcccg acgggacgat cgagctgacc ttcctcgggt cggcgggcca
     2221 gagcttcggt gcgttcgtgc cgaagggcat caccctacgg ctcgaaggcg acgccaacga
     2281 ctacttcggc aagggcctca gtggcggcac gctcgtggtg cggccaccgc gcaacgcgaa
     2341 gttcgtcgcg gaagagaaca tcatcgccgg caacgtgatc ttgtacggcg caacgggcgg
     2401 cttcacgttc atccgcggcg tcgtcggcga acgcttctgc gtgcgcaact cgggtgcgac
     2461 ggcggtggtc gaaggcgtcg gcgaccatgg ttgtgagtac atgaccggtg ggcgcgcggt
     2521 gatcctcggt ccgaccggac gcaacttcgc ggcgggcatg tcgggcggct tcgcgttcgt
     2581 ctgggatccc gaccgttcgc tctaccgccg catgaatcac gacatggtcg acatcgacac
     2641 gctcgacgac gaagacgccg actggttgcg cggcgtcgtc acgatgcaca tggaagagac
     2701 gggttcgccc gtcgccgagc gcatcttgtc gaagtggtgg aggaacgtca gctcgttcgc
     2761 caagatcttc ccgaaggact acaagcgggt gttggaagca caacgcgacg ccatcgagcg
     2821 gggcgtcgac gtcgacgaag cgatcatggc ggcagctcgt gggtgagacg acgggcttca
     2881 tgaagtacga ccgggagctg ccgaaacggc ggtcgatccc ggtgcgcgtg ctcgactggc
     2941 gggaggtcta cgaggacttc ccggccgaca aggtgcgcac ccagggtgcc cgctgcatgg
     3001 actgcggcat cccgttctgc cacaccggct gcccgctcgg gaacctcatc ccggagtgga
     3061 acgacctggt gtaccgcgac cgttggcaga acgcggccga ccggttgcac gcgacgaaca
     3121 acttccccga gttcaccggt cgactgtgtc ccgcgccgtg cgaagcggcg tgcgtgctcg
     3181 gcatcaacga gccgccggtg acgatcaagc agatcgaagt cgagatcgtc gatcgcgcgt
     3241 gggaagaggg ctgggtcaca ccggttgtgc ccgagaagcg cacgggcaag gaggtcgcgg
     3301 tcgtcggcag tggtccggcc gggctcgcgg ctgcgcaaca actgacgcgc gcgggtcatg
     3361 aagtcgtcgt gtacgagcgc gccgatcgcg tcggcggcct gctgcgctac ggcatccccg
     3421 agttcaagat ggagaagcgt cacctcgacc agcgcgtcgc gcagatggaa gccgaaggca
     3481 ccgcattccg cacgaacgcg aacgtcggcg acaacgtccc cgtcaacgag atcctcggct
     3541 acgacgcggt cgtgctcgcg ggtggtgcga cggcgtggcg cgacctaccg gtgccgggcc
     3601 gtgaatacaa gggcgtctac caggcgatgg agttcttgcc gtggggcaac cgggtgcagt
     3661 tcggcgacgt cgtcgaggcg cccatcaccg ccaaggacaa gcgtgtcgtc atcatcggtg
     3721 gcggcgacac cggcgccgac tgcctcggca cgtcgatccg ccaaggcgcg gcatcggtgc
     3781 accagttcga aatcctgccg cggccgccgg atgcgcgcgt cgactcgatg ccgtggccga
     3841 cgtacccgat gatctaccgc gtctcgtccg cgcacgaaga gggcggcgag cgcgtgtacg
     3901 cggtgaacac cgaatgcttc ctcggtgacg acgacggcaa cgtgcgagcc ctgcgcgcgc
     3961 acgaagtcga gcagaagatc gtcgacggcc gcatgacctt cgagaaggtc gaaggcaccg
     4021 acttcgagct gccgtgtgaa ctcgtgctgc tcgcaatggg tttcgtcggc ccgcagcgcg
     4081 aaggcctgct cgaccagctc ggtgtcgacc tcaacgagcg cggcaacgtg tcacgcgacg
     4141 gtcgctacaa gacgaacctc gacaacgtct tcgtcgccgg cgacatggga cgcggccaga
     4201 gcctgatcgt gtgggcgatc gccgaaggcc gctcgtgcgc gtcggccgtc gacgagtacc
     4261 tcatgggctc gacgctgctc cccgcaccga tcagccccga cgccgtaccg atgcgctaag
     4321 tcacggtcat ctcgaagggg agggtgtaca gcgggcgcca gtggtcgttg agccacgtgt
     4381 agtagacgat gacgcccgga tacttgccgg ggaacgcgta ggtgtgcgtc acgggcgcgg
     4441 ccgcgccgac cgacgtgccc tggtcgacga tcgtgccgtc accgaggtac acgaccttct
     4501 tcgtcgcgta gaggtcgaag ccgggatcgg tgaagccgtg ctcgacgacc gtcggttgtc
     4561 cgtgcgttgc cgtcatcgac tcccagagcg tcgacaccgg gccgccgtag ccgctcgggt
     4621 cgacgatgaa ctggccgctc tcgtcgtcgt agaacgagcc gttgtgcacg tgcaggttcc
     4681 agttgaccag caccagtccc gacggcagcc gctccatcgt gccgctcaca gtgcacggga
     4741 acgagacgag gcacaacgtc ggtgtgtacg ccgtcacctc ccgccgccag cgcgcaacac
     4801 cgttggagta atcgccggcg accgacacga ccatgcgcgt cggttcgttg ccctcgtcgg
     4861 ggttcgacgg cccgaacgcg atccggtagc gcgtcccgtc gagcccggtg acaaccgtgc
     4921 cggtggcgaa cgcgtacttc gacgtctcga cgtccatcgt gttgtcggtg ccgctcgtcg
     4981 tcagtgagaa cgaaccggtt gtggccggcg cggggttggg attcgacagc gcacccatca
     5041 cgaggttgcc ggtcggcggc aagtggccgc gctgccagat cgccttgtgg aactcgaacc
     5101 cgggccgccc cgcgaagtcg ggaacgcacg ccggacggtc gcggcaatac atgtcgtcgc
     5161 ggatggtctg gttccagaac aagtcgagca ggaacgtcgt cttgttcccc gccacgcaac
     5221 acgtcggcgc gtcgtcgttg ccgaacatct ttcccgtgtt gatctggttg gccagcgccc
     5281 attcgtcgtc gaagttgttg aggatctcgg tgccgttcca atacggcaac ggcatcaggt
     5341 aaacgctgcg ccggcccgtt tgcgagtact gccactcacc cggaccttca gggttgtagt
     5401 aggcgaagtg cttgaaccag cgcgcgcccg acaggaacgt gccgtcggct tgccgttcac
     5461 cgaagcgcaa cgtgatcttc tggctcgagc tcgcggtctg caacgcccat gcggtttgat
     5521 cgacaccgag gcgcgcgacc gtcgtgttct gcagcgtcat gcggaacggg agatcgacgt
     5581 cgatgcacaa gtcgatgtca gatgtgtcga agacggtgag gatctcgtcc ttgtaaccga
     5641 agccgacgtt cttcgccttg gcgtacacgg tcccgttcac cggttgatcg gtcatcagct
     5701 cggcttcgag ccggccgaag acgtcgaccc gggcccgcgc gacgacactc gtcgccacgc
     5761 cgacgtcgac ttcggcgtgg atgtactcca cacccatcga accgcgcccg acgtaattgt
     5821 ggtcgggaca gacgttgtga tgcgacggca tcgactcgtt gccctggtac acgagctccg
     5881 catggatcgc gtgctcgaca ccggtcagtg agacgtcatc gttgttcgtc cgcgtcttgg
     5941 acgagcccgc gccgacgtag gcgggtgacg cgtcgtcgag caccgtcgcg tgcaccgcgc
     6001 cgatggttgg gaagagcgcc gggttgctcc ccagcgtgtc gatctcgaac ttggcgtcga
     6061 ggttcggtgc ccgccggttg gtgacgaagc tcgcgagcac ttgcaccatg ccgggcacgt
     6121 ggtcgacgtg ccccgcgatg tcgaggtcgc acaccttctt cccgccgggg ccgacgaggc
     6181 actcgttcgg ggtcgcgggg gagtcggggt cgtcggtcgt gtgatgcccg cggatacgca
     6241 cgagcacgtc ggccgcgccg tactctgcga tcgtgctcag gatcttcatg gagacgaagg
     6301 tccgcgcgtc gcgcgtgaac atctgttgcg actgacacgc gttgatctcg ttcgggggag
     6361 tcgagtcgtc gcacacgtcg aggttcggcc ggtacgccac gagctgtgtg gggatgggga
     6421 cccgcgccgc gacgtcgacg ccggtcacgt cggcggtgcc gtcactgagc acgtgcaccg
     6481 tcgcgccgag gggcgtcgcc gcggtcggct tgccgagccc gatgctcgct ttcccgagcg
     6541 gatcaatgtc catcgtctgg tgcgcggccg tcgcgccgat ctttgcgagc acgcggtact
     6601 cgaacgcgga tgcctcgatt gccgagccgg tcggttgggt cgtgccgtcg cagcgccgag
     6661 tatcgatcca caacagcggg atgctgagcg ggttgttggc cgagccttcg tcgttgaccc
     6721 gcgcgtccag cacggtcggc agctggttga gtattccgtc gacgtatatc gggtcgatgg
     6781 gtaggccctt gcggtcgacg aggccgctca cgaacacgcg cgtcccgaaa cgcttgtcgg
     6841 tcaccgtctt cggcagcgcg ctcgtctccg cgcacacctt ccgtccggta gtgaggctcg
     6901 ggtagtcgta gcgaacgcgc ttgaccttcg acagctcgac gcgcgcaccc caatcgtccg
     6961 ttccgttctg cacatacgtg atgccgaatg gtgtgctcgt gaatgcggga tacgtcgccg
     7021 ccggttgcac agcgggcagc gcgggcgcgc tgaggatccc gatcgcttca cccaacaccg
     7081 gcgggccgta gacggcgcgc gccgacaccg cgtcgaacac ggtgcccgca gtgcaggggg
     7141 cgacgtcaca cggccgcagc tcgaagtgcc gcagcgacgc gtccgtgcgt tgccattctg
     7201 cgacgagccg cgtgcccagt gtgacgtcac cccacacccg cgtgcggcgc tgcttggccc
     7261 ggtccgcact ggtactggtg acgaagccgg ccctcagccg cgtcttgctc gacgccgtgt
     7321 agtcgagccg tgagagcccg gtcgcggtcg gcgccgcggt gtacgtcatc gtcgccttcg
     7381 ccggcatgcc cgcggtggcg tcgccgagca gaccgcgccc ggccacccgc aggaggctgt
     7441 tcgcagtgtt gccctcgtag gtgaacgacg cgtcgaagat gccctgcgca ccgaagagct
     7501 ggtagctcgc gctgaacggc acggcattcg tcgacgtgta gccgaacgtc gcgtgcgcgg
     7561 ggaccggcgt gagcgtgccg ttgagcacga accgggcgcc tttgacgagc gcctgcaggt
     7621 tcgcatcgag gtcgtagtgc cgcgtgtcgc tgtcgaagac gaccttcgcg gacggtaccg
     7681 acgacgcgag ctggagctcg gcgaacgcgc tgctcgtcgt gttgaggacg ggtgggccga
     7741 agtgcacgtg ccgcacgccc ggcatccggg ccgcgaaccg gaagcgctgc tcttgcgtca
     7801 gcgacagctg cccgccgatg tcgcggttcg cgtacgcgac gatcggaccg ttttgcagcg
     7861 caaacgtggg gagcgaggtc gtctcgcccg ggagcacgtt cgacgcgacg agcccgatct
     7921 cgccgaccgt gccgagcgtg tcgttgcact cgttcgaggc ccacgcgccg tcgcagaagc
     7981 gcacgtcggc ttcttggaac gcgccggttg cgtcgcgcag cacgtcgacg ttcgtgacgg
     8041 gtggcaggtc gctgacgaac ccgttgactg cgagccgcgt gtcggcgtcg cccggtcccg
     8101 acgacagcgc ggtgaccacc aactcgccga cgtcgaggtc gggcgccggt gacgacggcg
     8161 cgctgttgcc gttgccgtcc gggtgttgct ggtggaacag gcggatgttc gtctcggtga
     8221 gtcgcgccgt catctcttcg ggtaccgcgt gcacggtgcc ggcgagatgc agctcgggtc
     8281 ggccggcaac gcccttcgcc ttcccaatgg tgagttcgac atctgcgtcg ctcggcgcga
     8341 cggtgtcgtc ggcaacgccg ggctgctcgt ctcggtcatc ccacgcgaac gtgcggttcg
     8401 gcgccgggcc ggtgcatgtc gtcgcgtcga cgaaggcgaa ctgcgcgggc atgcgggccc
     8461 agtcgatcga caggtcgagg tcttcgtcga cgacgttgaa gttgccgacc tttacgaagg
     8521 tgttgagcgg cggttcggaa ccgggcgtga cgagcgcggc ggccggatcg cctccggcgc
     8581 ggtactgcga cgagatgcgt gcgccgaggt agcccgaatc gggcgcggcc ggcggtgcgt
     8641 cctggaacac gacggtcatg tcgagatggg tcggtggcgc ggcaccggtc gggctcgtgt
     8701 cgtagccgag attcactgcg gtcgtcgtcg ggttgccgcc gttgacgagg tcgaagagag
     8761 cgccaccgtt gatgcggaac ggcgcaaccg acaggtcggt cgcgagccgg tcgaaggaga
     8821 cgcggaccgc ggtgtacggg ttcgacgcgt cgagcgcact cccgacgcgg tcccacagcg
     8881 gctcgaggtt cgtcgcggac agatcggtgg cgccgagctc gaggttccac tggaggtcgg
     8941 gtgtgccgtc ggcgtcggtg tcgaggttgc gcgccgccgg tgtcccgagc gcggacgcca
     9001 gctgcggcag gttcaacacg atgacggtcg gcggaaggtt ggggttcgcg ggcgtgatct
     9061 ggaacgcgac gaagtggatg gtgccgagct cggtgtcggg gccggccggg cagccgacca
     9121 ggaacccgtc cgcgttcgct cgcggcaccc gcgcgccgac gaggaccacg gccaacgcgg
     9181 tcaccacgag ggctcccgcg agtgcccacc gagtcgctgt cctgatcacg ccgcacccct
     9241 tccgtcgtcg cgctcggggc aacgtacggg cgggcgatca catgacc
//
LOCUS       DASUGA010000453         6571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1401094, whole genome shotgun sequence.
ACCESSION   DASUGA010000453 DASUGA010000000
VERSION     DASUGA010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401094"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..766
                     /locus_tag="VFX21_08980"
     CDS             146..766
                     /locus_tag="VFX21_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096134.1"
                     /translation="MTQIPQESKARLVAVFTSEDEVRTASRALQQAGVDASRLTVDRG
                     ADHLSSVGSEMREEINESVMGPGSVGPFTRNMSRGMFTGTFVGGVIGLVLALPFAAFS
                     FGDWPVWLRLLVVAIVGATLGATAGWVIGGGFGADRDDETLAAEHGITLGVPASETAQ
                     EVLRAAHPLRIDLVAANGKPIATVLAREEPITRTLGRHLADEERHD"
     gene            complement(785..1063)
                     /locus_tag="VFX21_08985"
     CDS             complement(785..1063)
                     /locus_tag="VFX21_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096135.1"
                     /translation="MGSFYDEVLRELLLALGAALFLANAFALAKRRDDGERAAARSVA
                     RHRPGSPVRGYKRSGDDHDLAQAPVGRTVLYMVAGLVISIWALASLMS"
     gene            complement(1077..1766)
                     /locus_tag="VFX21_08990"
     CDS             complement(1077..1766)
                     /locus_tag="VFX21_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096136.1"
                     /translation="MSRDRRAGVALFAVVALALGITTAVTDSVFPPVRHDTDAYEALL
                     ALLDANESGTWIVDYDMQRTIAGGRHVATSYTEARDDEIHIVRGGGSADATIGDKRYL
                     CTDTEEAGTICNASPAVQSLASSVVVKTAVESGAYVVTTLPSDDIAGEHARCYRLFGA
                     GGYIPELGAETRYCFADDGVQLRTKITTVNDERDRVATRVVRDPSSTALEQVIGSLET
                     GAGSADGDDTN"
     gene            1788..2447
                     /gene="nucS"
                     /locus_tag="VFX21_08995"
     CDS             1788..2447
                     /gene="nucS"
                     /locus_tag="VFX21_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019855210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HEX5096137.1"
                     /translation="MRLLVARCAVEYQGRLGARLASATRLILFKADGSIAIHSDAKAY
                     KPLNWMNPPCVIDEDDNIITVRTPKGETLRIELEEVLHDTTVPLGVDPGLEKDGVEAE
                     LQQLLAARVTAMRDGLTLVTREYPTDIGPVDLLCIDENDQTVVVEVKRVGEIASVEQL
                     LRYQERLDRDPRFAPTAGLLVAQQIKPQAKVYAESKGVAWVEIDLERLRSDVDPDLTL
                     F"
     gene            2463..3302
                     /gene="purU"
                     /locus_tag="VFX21_09000"
     CDS             2463..3302
                     /gene="purU"
                     /locus_tag="VFX21_09000"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011242849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEX5096138.1"
                     /translation="MRAALLLRCPDRPGIVAAVGEFVAAVGGNIIEADQHSDVAADLF
                     LQRVEFDVDASLPVLQQEFEPIAVRLSMDWQLHDLAHPARIGVLVSRQGHCLGDLLVR
                     SALGELPGEITFVASNHDTNRAITERFGVPFRHVPVVGDDRDAQERALGAALDEARPD
                     VVVLARYMRVLPAWLANAWHERMINIHHSFLPSFAGARPYHQAHERGVKVIGATAHYV
                     TPELDEGPIIAQQVMPVSHRDDPARLERRGRDLEAVVLSEALRLHLEHRVIVYGRRTA
                     VFD"
     gene            complement(3272..4342)
                     /locus_tag="VFX21_09005"
     CDS             complement(3272..4342)
                     /locus_tag="VFX21_09005"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX5096139.1"
                     /translation="MYRTTGKLTRAVLVTAVLGAASLAVGGTAGAAPVSFPQVSCNAH
                     LSVPNIDTAVPIDFTFEAQAPASAAPGTPVTVTIPADTSDLPATNSGQTVTQYANIGV
                     DYGVTGGTIDAASVAAAGDTTINGAVTPAVASATADTLTTTVPGPIPPGTLATPAISF
                     NVVGTTAGTNVVISVKSQQTTATLDNAALGDAAVTCPINGTLATTEIVAAGAPTAADD
                     KAETQPGQAVTIDVRANDTASAELAIDETKFAITTAPTKGTATIVDGKIVYTPNAGVE
                     GTTDTLVYTLCSVPAADGTVPCDTATVTITIAAVQAAAPAAAAELPRTGSDTTALALG
                     ALAFVVLGLMLARQSKTAVRRP"
     gene            complement(4460..5290)
                     /locus_tag="VFX21_09010"
     CDS             complement(4460..5290)
                     /locus_tag="VFX21_09010"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX5096140.1"
                     /translation="MLAATLLALASAGLHATWNLIIKRADDRELAALGQFVAGAVIFA
                     PVLLLAGLPDRDAWPFLLASGVVHLAYIYALTTAYHHGDFSLAYPLARGGGALVAAVV
                     GAIVLGDALAAGAWCALVVVAVGLASLVQPGTPFAEIAYALLTAAVIGTYTVIDTAGS
                     RHTGNGFVYTCALMVVSATALAIAVAGRGRTGEFVTYLRGNLGVAVVSGIFLTLAYAL
                     VLIAVRFAPVGYVATLRESSVVLGAVLGWLVLGEAMGRRRTISAVVITTGLVGLIVFR
                     "
     gene            complement(5303..>6571)
                     /locus_tag="VFX21_09015"
     CDS             complement(5303..>6571)
                     /locus_tag="VFX21_09015"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5096141.1"
                     /translation="VLFFSMEMGHLELTKRLLAAESLIDSRKLSTGRLNEHEWPKLNQ
                     AVGRLAEAPFFIDDNPHTTVMEMRAKARRTRARYGDIGLIVVDYLQLMSSGSKRVESR
                     QVEVSELSRGLKILARELEAPVMCLAQLNRQLEYRQDKRPMLADLRESGCLVGATEIA
                     LADGTTATMRELYDSGARGIEVLTLDEHLRLETGVMTHVFPSGSKPTFELRLASGRSV
                     TASGNHPFLTLDGWVQVADLRPGARIASARTMGTIDPARDTIPPAVWDYIEHKGLLGM
                     GMRAHDLIERLGSDVGSHRAYEEGIGRVLMQRVAGMYCDPFLHDLADSDVLWDEVTTI
                     VPRGNEWVFDATVLGTHNFVANGVVAHNSIEQDADVVLFIYRDEYYNPESDQRGMAEI
                     IVSKHRNGPVGTTRLAFLEQYTKFANLARE"
BASE COUNT         1008 a         2269 c         2277 g         1017 t
ORIGIN      
        1 cgcgcccggc cgcgaggacg tcttgctccg tgtcgccgcg cagctcgaaa ccgccgcaga
       61 ttggtcgtcg cgccgtccgc cgatctcctg atccggtttc catcgttgcg cgcccgggaa
      121 cgcgcgcgac gacaaggaga ccgtcatgac gcaaatacct caggagtcga aggcgcgact
      181 cgtcgccgtc ttcacgtcgg aggacgaggt gcgcaccgcg tcgagagcgt tgcaacaggc
      241 gggcgtcgac gcgagccgcc tgaccgtcga ccgcggcgcc gatcacctgt cgtcggtggg
      301 cagcgagatg cgcgaagaga tcaacgagtc ggtcatgggc ccgggcagcg tcggaccgtt
      361 cactcgcaac atgtcgcgtg gcatgttcac ggggacattc gtcggcggcg tgatcgggtt
      421 ggtgctcgcc ctgccgttcg ccgcgttctc gttcggtgac tggcccgtct ggctgcggct
      481 gctggtggtc gcgatcgtcg gcgccaccct cggcgcgacc gccgggtggg tgatcggcgg
      541 tggcttcggc gccgatcgcg acgacgagac gctcgcggcc gaacacggca tcaccctcgg
      601 tgtgcccgcg agtgagactg cgcaggaagt gctgcgcgcc gcgcatccgt tgcgcatcga
      661 cctcgtcgcg gcgaacggca aaccgatcgc gacggtcctc gcgcgcgagg agccgatcac
      721 gcggacgctc ggccggcact tggccgacga agagcgccac gactgactcg gccgcgggcg
      781 cgcgtcacga catcagtgac gcgagagccc agatcgagat gacgaggccg gcgaccatgt
      841 agagcacggt gcggccgacg ggcgcttggg ccagatcgtg gtcgtcgccg ctgcgcttgt
      901 agccgcgcac gggactgccg ggccggtgcc gcgcgaccga ccgggcggcg gcgcgctcgc
      961 cgtcgtcgcg ccgcttcgcc aacgcaaacg cgttcgcgag gaacaacgcg gcaccgagcg
     1021 cgagcagcag ctcgcggagt acctcgtcgt agaacgaacc catagcgggt gaaacgctag
     1081 ttcgtatcgt cgccgtccgc cgagccggcc ccggtctcga gggagccgat gacctgctcg
     1141 agggcggttg acgacgggtc gcgcaccacc cgggtcgcga cgcggtcacg ctcgtcgttc
     1201 acggtcgtga tcttcgtccg cagctgcacg ccgtcgtcgg cgaagcagta ccgcgtctcg
     1261 gcaccgagtt cggggatgta cccgcctgca ccgaagagcc ggtagcaccg cgcgtgctca
     1321 cccgcgatgt cgtcgctcgg gagcgtcgtc accacgtacg cgccgctctc gacggccgtc
     1381 ttcacgacga ccgacgacgc gagcgactgc accgccggtg acgcgttgca gatggtgccc
     1441 gcttcctcgg tgtcggtgca caggtagcgc ttgtcgccga tcgtcgcgtc ggccgaaccg
     1501 ccgccgcgca cgatgtggat ctcgtcgtcg cgtgcctcgg tatacgacgt cgcgacgtgg
     1561 cgtccgccgg cgatcgtccg ttgcatgtcg tagtcgacga tccaggtgcc ggattcgttg
     1621 gcgtcgagca gcgcgaggag cgcttcgtac gcatcggtgt cgtgccgcac gggcgggaac
     1681 accgagtcgg tgacggcggt cgtgataccg agcgcgagcg cgacaacggc gaacagcgct
     1741 acacccgccc ggcggtcccg cgacacgccc caggatactc tccgaccgtg cgcctgctcg
     1801 ttgcccgttg cgcggttgag taccaaggcc ggctcggcgc gcggctcgcg tccgccacgc
     1861 ggctcatcct cttcaaagcc gacgggtcga tcgcgatcca ctccgacgcc aaggcctaca
     1921 agccactgaa ttggatgaac ccgccgtgtg tcatcgacga ggacgacaac atcatcaccg
     1981 tgcgtacacc gaagggtgag acgctgcgca tcgagctcga ggaagtcctg cacgacacga
     2041 ccgtgccgct cggtgtcgac ccgggactcg agaaggacgg cgtcgaggcg gaactgcagc
     2101 aactgctggc cgcgcgcgtc accgcgatgc gcgacgggct cacgctggtc acgcgtgagt
     2161 acccgaccga catcgggcct gtcgacctgc tctgcatcga cgagaacgac caaaccgtcg
     2221 tcgtcgaggt gaagcgcgtc ggcgagatcg cgagcgtcga gcagttgctg cgctaccagg
     2281 aacgactcga ccgcgatcct cggttcgcgc ctaccgccgg gctgctcgtg gcgcagcaga
     2341 tcaagccgca ggccaaggtc tacgccgaga gcaagggcgt cgcgtgggtg gagatcgacc
     2401 tcgaacgcct gcgcagcgac gtcgaccctg atctcacgct gttctaggcc gattgttcct
     2461 gcatgcgggc cgcgctgctg ctccgttgcc ccgatcggcc cggcatcgtc gccgcggtcg
     2521 gcgagttcgt cgcggccgtc ggcggcaaca tcatcgaggc cgaccagcac tcggacgttg
     2581 ccgccgatct cttcttgcag cgcgtcgaat tcgacgtcga cgcgtcactg cccgtgctgc
     2641 aacaagagtt cgaaccgatc gcggtccgcc tgtccatgga ctggcagctg cacgacctcg
     2701 cgcatccggc ccgcatcgga gtgctggtgt cgcgtcaggg ccactgtctc ggcgacttgc
     2761 tcgtccgcag cgcgctcggc gaattgccgg gtgagatcac gttcgtcgcg tcgaatcacg
     2821 acaccaaccg cgcgatcacc gaacgcttcg gcgtcccgtt ccgtcacgtt ccggtcgtcg
     2881 gggacgatcg cgacgcccag gaacgtgcgc tcggggcggc cctcgacgag gcccgccccg
     2941 acgtcgtcgt gctcgcgcgt tacatgcgcg tgctcccggc ctggctggcg aacgcgtggc
     3001 acgaacggat gatcaacatc caccactcgt tcctgccctc gttcgccggg gcgcggccct
     3061 accaccaggc ccacgagcgg ggcgtcaagg tcatcggcgc gaccgcgcac tacgtgaccc
     3121 ccgagctgga cgagggaccg atcatcgccc agcaggtgat gccggtctca caccgcgacg
     3181 atcccgcgcg actcgaacgg cgcgggcggg acctcgaagc cgtcgtgctc tccgaggccc
     3241 tccgcctgca cctggaacat cgagtgatcg tctacgggcg ccgcaccgcc gtcttcgact
     3301 gacgggcgag catcagaccg agcaccacga aggcgagcgc accgagcgcg agcgccgtgg
     3361 tgtcactgcc agtgcgcggc agctccgccg cggccgccgg tgcggccgcc tggacggccg
     3421 caatcgtgat cgtcaccgtc gcggtgtcgc acggcacggt cccgtccgcg gccgggaccg
     3481 agcacagcgt gtagacgagc gtgtcggtcg tgccctcgac acccgcgttc ggcgtgtaaa
     3541 cgatcttgcc gtcgacgatc gtggcggtcc ccttcgtggg tgccgtcgtg atcgcgaact
     3601 tcgtctcgtc gatcgccagc tcggccgaag ccgtgtcgtt ggcgcgcacg tcgatcgtca
     3661 ctgcctgccc cggctgcgtc tcggccttgt cgtccgcagc ggtcggcgcg ccggccgcga
     3721 cgatctccgt cgtcgccagc gtcccgttga tcgggcaggt caccgcggcg tcaccgagcg
     3781 cggcgttgtc cagagtcgcg gtcgtctgct gagacttgac gctgatcacg acgttcgtac
     3841 ctgccgtcgt gccgacgacg ttgaacgaga tcgcgggcgt cgcgagcgtg ccgggcggaa
     3901 tcgggccggg cacggttgtg gtcaacgtgt ctgccgtcgc actggcaacc gcgggcgtca
     3961 ctgcgccgtt gatcgtcgta tcgccggcgg ccgcgaccga ggccgcgtcg atggtgccac
     4021 cggtgacgcc atagtcgacg ccgatgttgg cgtactgggt caccgtctgg ccgctgttcg
     4081 tcgccggcag gtcgctcgtg tccgcgggaa tggtcaccgt gaccggcgtg cccggcgccg
     4141 ccgacgcggg cgcctgggcc tcgaacgtga agtcgatcgg gacggccgta tcgatgttcg
     4201 gcacgctcag atgcgcgttg cacgacactt gtggaaacga gacgggcgcc gcgccggcgg
     4261 taccgccgac cgcgagcgat gccgctccca agaccgcggt caccaagacc gcgcgcgtca
     4321 gcttgcctgt ggtcctatac acctcggtct ccccctcgcc cggattcatt ccaggatttg
     4381 ggcgggaccg tacccactgt ccgcgcgggc gtctacctgg tcagttcacg cgaagtgacg
     4441 cgaatctggc ctttctcctt catcggaaga cgataagtcc gacgagcccg gtggtgatga
     4501 cgacagccga gatggtccgg cggcggccca tcgcctcgcc cagcacgagc cagccgagca
     4561 ccgcgccgag cacaaccgac gactcccgca acgtcgcgac gtaaccgacc ggcgcgaacc
     4621 gcaccgcgat gagcacgagc gcgtaggcca gggtcaagaa gatgccggac acgaccgcga
     4681 cgccgaggtt gccgcgcagg tacgtcacga actccccggt acggccccgc ccggccacgg
     4741 cgattgcgag ggccgtcgcg gagacgacca tgagcgcgca cgtgtacacg aagccgttgc
     4801 cggtgtggcg cgacccggcg gtgtcgatca cggtgtaggt gccgatcacc gccgcggtca
     4861 gcagcgcgta ggcgatctcg gcgaacgggg tgcccggttg caccaacgac gcgagcccga
     4921 cggcgacgac gacgagcgcg caccacgcgc cggcggcgag cgcgtcgccg agcacgatcg
     4981 cgccgacgac cgcggcaacg agcgcgccgc ccccgcgcgc gagtggatac gcgagcgaga
     5041 agtcgccgtg gtgataggcg gtcgtgagcg cgtagatgta cgcgaggtgc acgacgcccg
     5101 acgcgagcag gaacggccag gcgtcacggt ccggcaggcc cgcgagcaac agcaccggcg
     5161 cgaagatcac cgccccggcg acgaactggc ccagcgcggc gagctcgcgg tcgtccgccc
     5221 gctttatgat caggttccac gtcgcgtgca gcccggccga cgcgagcgcg agcaacgtgg
     5281 ccgccaacat cggccgcgga cgctactcgc gcgcgagatt cgcgaacttg gtgtactgct
     5341 cgaggaacgc caggcgcgtc gtgccgacgg gaccgttccg gtgcttcgac acgatgatct
     5401 cggccatgcc gcgctggtcg gactccgggt tgtagtactc gtcgcggtag atgaacagca
     5461 cgacgtcggc gtcctgttcg atgctgttgt gcgcgacgac gccgttggcg acgaagttgt
     5521 gcgtgccgag cactgtcgcg tcgaacaccc actcgttgcc gcgcggcacg atcgtcgtca
     5581 cttcgtccca gagcacgtcg gagtcggcga ggtcgtgcag gaacgggtcg cagtacatgc
     5641 cggccacgcg ctgcatgagc acgcggccga tgccttcttc gtacgcacga tgcgaaccga
     5701 cgtcgctgcc gaggcgctcg atgagatcgt gcgcgcgcat gcccatcccg agcagtccct
     5761 tgtgctcgat gtagtcccac acggcgggcg ggatggtgtc gcgcgcgggg tcgatggtgc
     5821 ccatcgtgcg cgcggacgcg atgcgggcac cgggacggag gtcggccact tgcacccaac
     5881 cgtcgagggt caggaacgga tggttgccac tcgccgtgac gctccgtccc gatgccagcc
     5941 gcagttcgaa cgtgggcttc gaaccgctgg ggaagacatg ggtcatcact ccggtctcga
     6001 gccggaggtg ctcgtcgagg gtgagcacct cgatgccgcg agcaccggag tcgtagagct
     6061 cgcgcatcgt cgcggtggtg ccgtcggcga gcgcgatctc ggtcgcgccg acgaggcaac
     6121 ccgactcccg caggtcggcc agcatcggcc gcttgtcctg gcggtactcg agctggcggt
     6181 tcagctgcgc gaggcacatc accggcgctt cgagctcgcg ggccaggatc ttcaggccgc
     6241 gtgacagctc cgacacttcg acctggcggc tctcgacgcg cttggagccg ctcgacatca
     6301 gctggaggta gtcgacgacg atgagcccga tgtcgccgta gcgcgcgcgt gtgcggcgcg
     6361 ccttggcccg catctccatg acggtggtgt gcgggttgtc gtcgatgaag aacggcgcct
     6421 ccgcgagccg gccgacggct tggttgagct tcggccactc gtgctcgttg aggcggccgg
     6481 tggagagctt gcgcgagtcg atgagcgact ccgccgcgag caggcgcttg gtgagctcga
     6541 ggtggcccat ctccatgctg aagaacaaca c
//
LOCUS       DASUGA010000454         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_909351, whole genome shotgun sequence.
ACCESSION   DASUGA010000454 DASUGA010000000
VERSION     DASUGA010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909351"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1433
                     /locus_tag="VFX21_09020"
     CDS             <1..1433
                     /locus_tag="VFX21_09020"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX5096142.1"
                     /translation="SFLLRDVAVPIYGSELSLALARHRIEEGGVLERTELIPVHDGER
                     RSIGPFDCQFIPVTHSVPHGFATAFFTPVGTIVHTGDFKIDLTPVDGRRTDLALLGEL
                     ARRDGGVRLLLADSTNAERPGFTPSESTVGSAMRALFREHPTKRFIVASFASHLHRVQ
                     QVAQAAISHGRKVAFVGRSMEQNVSLSRERGFIDIPSHAVIDIEETARYPAGDVCIIC
                     TGSQGEPMSALSLMAAHEHKHVKVSEDDVVVISAHAIPGNEANVSRVIDSLHRAGAEV
                     VHDRNYPVHVSGHASQEELKFILNLLSPDWFVPVHGEYRHLVHHAELAEHSGVASDHV
                     LVCEDGDLLTIGAEGIEVERRAVPAGYMYVDGIVGDIGQGVLRDRRVLAEEGVVVVIV
                     TIDATTGEVVAGPEIVTRGWVYAPEAEDLLEEAQQVVRASLDEAAREGATDFETMRRH
                     ARRSVGRFINERTRRRPIVIPVVMEV"
     gene            1433..2194
                     /locus_tag="VFX21_09025"
     CDS             1433..2194
                     /locus_tag="VFX21_09025"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5096143.1"
                     /translation="MVAIDVSGRRALVTGGGNGLGTEICRALSHAGAFVYVNDIYEDR
                     AQKVVEELGGVDVAQAVKADVTSPPKVQRMRAETGPVDILVNNAGIPTSGFDLKRFVD
                     TNPDDWEPMMRLNLGAILHVTHAYAGAMVDAGWGRIVTIVSDAGRKGERFQAIYGSAK
                     AGAMGFMRGLAAEVGAFGVTANCVSLGTMRSGFSVDAIENNPELEQRLSKPYTVPRLG
                     EPADVAPLVALLCSDAGAWITGQVYPVDGGYVSAL"
BASE COUNT          355 a          765 c          797 g          347 t
ORIGIN      
        1 tgtccttctt gctgcgcgac gtcgcggtac cgatctacgg gtcggagctg tcgctcgcgc
       61 tggcgcggca ccgcatcgaa gaagggggag tgctcgagcg caccgagctg atcccggtgc
      121 acgacggcga gcggcggagc atcgggccct tcgactgcca gttcatcccc gtgacgcact
      181 cggtgccgca cgggttcgcc accgcgttct tcacgccggt cggcacgatc gtgcacaccg
      241 gcgacttcaa gatcgacctc accccggtgg atggccggcg caccgatctc gcgctgctcg
      301 gagagctggc ccgccgcgac ggtggcgtgc gcttgttgct ggccgactcg accaacgccg
      361 agcggccggg cttcacgccg tcggagtcga cggtcggcag cgcgatgcgc gcgttgttcc
      421 gtgagcatcc gacgaagcgg ttcatcgtcg ccagcttcgc gtcccacctc caccgggtgc
      481 agcaggtcgc gcaggccgcg atcagccacg ggcgcaaggt cgcgttcgtc ggccgctcga
      541 tggagcagaa cgtgtcgctc agccgcgagc gcggcttcat cgacatcccg tcgcacgcgg
      601 tcatcgacat cgaggagacc gcgcgctatc ccgcgggcga cgtgtgcatc atctgcaccg
      661 gttcgcaggg tgagccgatg agcgcgctgt cgctcatggc cgcgcacgag cacaagcacg
      721 tgaaggtctc cgaggacgac gtcgtcgtca tcagcgcaca cgcgatcccc ggcaacgagg
      781 cgaacgtctc acgcgtgatc gattcgctgc accgcgccgg tgccgaggtc gtgcacgacc
      841 gcaactatcc ggtgcacgtc tccggccacg cgtcgcagga agagctcaag ttcatcctca
      901 atctgctgtc gcccgactgg ttcgtgccgg tgcacggcga ataccgccac ctcgtgcacc
      961 acgccgagct ggccgagcac tcgggcgtcg catcggatca cgtgctcgtc tgcgaggacg
     1021 gcgacctgct cacgatcggc gccgagggga tcgaggtcga acggcgggcg gtgccggcgg
     1081 gctacatgta cgtcgacggc atcgtcggcg acatcggcca aggtgtgttg cgcgaccggc
     1141 gcgtgcttgc ggaggagggc gtcgtcgtcg tcatcgtgac catcgacgcg acgaccggcg
     1201 aagtcgtggc cgggcccgag atcgtgacgc gcggctgggt gtacgcgccc gaagccgaag
     1261 acctgctcga ggaagcgcag caagtcgtgc gcgcgtcgct cgacgaagcc gcgcgcgaag
     1321 gcgcgaccga cttcgagacg atgcggcgcc atgcgcgccg ctcggtcggc cggttcatca
     1381 acgaacgcac gcgccggcgg ccgatcgtca tccccgtcgt catggaggtc tgatggtcgc
     1441 gatcgacgtc tcgggccggc gcgcgctcgt cacgggcggc ggcaacgggc tcgggacgga
     1501 gatctgccgc gcgctgtcgc acgcgggcgc gttcgtctac gtgaacgaca tctacgagga
     1561 ccgcgcgcag aaggtcgtcg aggagctcgg cggcgtcgac gtcgcccaag cggtcaaggc
     1621 cgacgtgacg agcccgccga aggtgcaacg catgcgggcc gagacggggc ccgtcgacat
     1681 cctcgtgaac aacgcgggca tcccgacgtc gggcttcgac ctcaagcgat tcgtcgacac
     1741 caaccccgac gattgggagc cgatgatgcg gctcaacctc ggtgcgatcc tgcacgtcac
     1801 gcacgcgtac gcgggcgcga tggtcgacgc gggctggggc cgcatcgtga cgatcgtgtc
     1861 ggacgcgggc cgcaaagggg aacggttcca ggcgatctac ggctcggcca aagccggcgc
     1921 gatgggtttc atgcgcggcc tcgcggcgga ggtcggcgcg ttcggagtca ccgcgaactg
     1981 cgtgtcgctc ggcacgatgc gctcgggctt ctcggtcgac gcgatcgaga acaacccgga
     2041 gctcgagcag cggctgagca agccgtacac cgtgccccgc ctcggcgagc cggccgacgt
     2101 cgcgccgctc gtcgcactgt tgtgcagcga cgcgggagcg tggatcaccg gccaggtgta
     2161 tcccgtcgac ggcggctacg tctccgccct gtagcgattt cggtcgggcg ttcttctgca
     2221 gcgaaattcg ctgcagaaaa acgcccgatg atcgttactg gcgg
//
LOCUS       DASUGA010000455         3658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_327017, whole genome shotgun sequence.
ACCESSION   DASUGA010000455 DASUGA010000000
VERSION     DASUGA010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327017"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VFX21_09030"
     CDS             <1..946
                     /locus_tag="VFX21_09030"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX5096144.1"
                     /translation="FTHNGFFWTGVVGYRGSYLNAIRNGDYGIYAFDSTMGRFDNDYA
                     SGSPDAGFYIGQCSPCDAVITDVIAEWNGIGYSGTNAGGNLYIVNSAWHDNRVGIVPN
                     SGTGEANPPQRDSVIAGNRVYSNNNAQTAAIDIAQTATYNGILLAGGNENVVVRNLVY
                     DHELVGIAPVVLPEKLLDPDNPKAQNFDARDNTIRENVLRDNRAADLALVTSIDDPND
                     AGGNCFAGNTYTTSLPADIETVAPCDASAAPPFTTDIGRFLSLLAAEKPAPADYKTVQ
                     LPDPGDQPQMENAQDAPPEPASKLRAPKIDLDDITLPN"
     gene            complement(930..1094)
                     /locus_tag="VFX21_09035"
     CDS             complement(930..1094)
                     /locus_tag="VFX21_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096145.1"
                     /translation="MPRAIVVVAAAVGGTLLASYSNGFEFFAFGGFWIWVFAAMLVIA
                     PARRRPNSEA"
     gene            complement(1175..2404)
                     /locus_tag="VFX21_09040"
     CDS             complement(1175..2404)
                     /locus_tag="VFX21_09040"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX5096146.1"
                     /translation="MRFRRRLAGVVIAVAAIVVPALPAAAGPSVDVYAYGSAPALGAP
                     DQALNGRIVDIAANPAGTGYWLLGLDGGVFSYGVDFFGSTGGLRLNAPVIGMAATPTG
                     RGYWFVAQDGGIFSYGDARFAGSTAGLPLQAPIVAIAASPTGNGYWIVSADGAVFAFG
                     DAQFHGGLAGVFTGQSIVSIAATPTGNGYVLLGDDGGVFSFGDAPQFTTWPMAQGARD
                     VAMTPSGQGIWVLGTDGGVFTYGDAPFYGAATDSGRNGVALAAAAGGYWIAAVPRDAD
                     GPPVPSDSGNGRRIVYSNSMQRVWLVEADGRVAHSYLVSGRRGVPSPGTYSVFSKSAM
                     SSAGSLRLPYMTRFAHGRSLAIGFHGIPLRANGSPIQSDDELGSYRSHGCVRMNQDAI
                     KVLYDWAPIGTVVVVLA"
     gene            2580..3518
                     /locus_tag="VFX21_09045"
     CDS             2580..3518
                     /locus_tag="VFX21_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096147.1"
                     /translation="MSASATIKNKLKTWQAGAGVMALGAVLLGAGLLVTTSAAPPADC
                     PEGSTLVSKYKANNGTYTFEKPAGNDNIVTLSNTTAASGDWSSTVPIVYMIVKAGPDS
                     ITTSFSPAQTSGSWDNLGFLVGSGQHPNISDVLFCESTTPATTTSTVTSSTTTTQAPT
                     STTTTAATTTSTAAPTTTSTLPSVTTSTGAATTTTTVAPTTTATAAPVTTTTVPIVVE
                     GTTTTVAAATSTTGPAGSTTTVANAVVTTTTTDPSQTGSNSAVVTTTSIGHSDTSSES
                     LPRTGGQSTPLVVTGLIFLVAGLAMTLLALNRRRTA"
BASE COUNT          659 a         1322 c         1198 g          479 t
ORIGIN      
        1 cttcacgcac aacggtttct tctggaccgg tgtcgtcggc taccgcggct cgtacctgaa
       61 cgcgatccgc aacggcgact acggcatcta cgccttcgac tcgacgatgg gccgcttcga
      121 caacgactac gcctccggca gccccgacgc cggcttctac atcggccaat gcagcccgtg
      181 cgacgcggtg atcaccgacg tgatcgcgga gtggaacggc atcggttatt ccggcacgaa
      241 cgcgggcggc aacctctaca tcgtcaattc ggcgtggcac gacaaccgcg tcggcatcgt
      301 ccccaacagc ggcacgggcg aagcgaaccc gccgcaacgc gactccgtca tcgccggcaa
      361 ccgcgtgtac tcgaacaaca acgcgcagac cgcggcgatc gacatcgcgc agactgcgac
      421 ctacaacggc atcctcttgg ccggtggcaa cgagaacgtc gtcgtgcgca acctcgtcta
      481 cgaccacgag ctcgtcggca tcgcaccggt cgtgctgccc gagaaactgc tcgaccccga
      541 caacccgaag gcgcagaact tcgacgcccg cgacaacacg attcgcgaga acgtgctgcg
      601 tgacaaccgc gcggcagacc tcgcgctcgt cacgtcgatc gacgatccga acgacgcggg
      661 cggcaactgc ttcgcgggga acacgtacac cacgtcactg ccggccgaca tcgagacggt
      721 cgcgccctgc gacgcctccg ccgcgccccc gttcacgacc gacatcggcc gcttcctgtc
      781 gctcctggcc gccgagaagc cggcgccggc cgactacaag acggtgcagt tgccggaccc
      841 gggtgaccag ccgcagatgg agaacgcgca agacgcgccg ccggagcccg cgagcaagtt
      901 gcgggcgccg aagatcgacc tcgacgacat cacgcttccg aattaggccg gcggcgcgcc
      961 ggtgcgatga ccagcatcgc cgcgaacacc cagatccaga agccgccgaa cgcgaagaac
     1021 tcgaacccgt tcgaatagct cgcgagcagc gtcccgccga ccgcagccgc gacgacaacg
     1081 atcgcgcgag gcatgcgggc cagttctatt ggcgcgtgga cgcgcgcgca cgggcgcgag
     1141 ttcttggccg ttcatcgcgc gcccgacaaa cgactcaggc gaggacaacc acgacggtcc
     1201 cgatcggggc ccagtcgtag agcaccttga tcgcgtcctg gttcatccgc acgcaaccgt
     1261 gactgcggta ggagccgagc tcgtcgtcgc tctggatcgg tgacccgttg gcgcgcagcg
     1321 gaatgccgtg aaaaccgatc gccagggaac gaccgtgcgc aaagcgcgtc atgtacggca
     1381 aacgcagcga gcccgccgac gacatcgccg acttcgagaa cacgctgtac gtgcccgggc
     1441 tcggtacgcc gcgccgaccc gacacgagat agctgtgcgc gacccggccg tcggcttcga
     1501 cgagccagac ccgctgcatc gagttcgagt agacgatgcg gcgcccgttc cccgagtccg
     1561 acggcaccgg cggaccgtcg gcgtcgcgcg ggaccgccgc gatccagtag ccgccggcgg
     1621 cggccgcgag cgcgacgccg ttgcggcccg agtcggtcgc ggcgccgtag aacggtgcgt
     1681 cgccgtaggt gaacacccca ccgtcggtcc cgagcaccca gatgccctgc ccgctcggcg
     1741 tcatcgcgac gtcgcgtgcg ccctgcgcca tcggccacgt cgtgaactgt ggcgcgtcgc
     1801 cgaaggagaa cacgccgccg tcgtcgccga gcaacacgta gccgttgccg gtcggtgtcg
     1861 ccgcgatcga gacgatcgac tgaccggtga acacgcccgc gagcccgccg tggaactgcg
     1921 cgtcgccgaa cgcgaacacg gcgccgtccg cggagacgat ccagtagccg ttgccggtgg
     1981 gtgacgccgc gatcgcgacg atcggcgctt gcagcgggag tcccgcggtc gaaccggcga
     2041 aacgcgcgtc gccgtaggag aagatgccgc cgtcctgcgc gacgaaccag tagccacgac
     2101 cggtcggcgt cgccgccatg ccgatcaccg gcgcgttcag gcgcagcccg cccgtcgaac
     2161 cgaagaagtc gacgccgtag gagaagacgc cgccgtcgag gccgagcagc cagtagccgg
     2221 tgccggcggg gttggcggcg atgtcgacga tgcgaccgtt gagcgcttgg tcgggcgcgc
     2281 cgagggcggg cgcgctcccg taggcgtaga cgtcgacact cggtcccgcg gccgcgggga
     2341 gggccgggac gacgatcgcc gcgactgcga tcaccacgcc cgccaggcgc cgccggaatc
     2401 tcacacccgt tccatcggcc acagtggccg acaattcaca gccggtgtcg caggagtgtc
     2461 aattccctgg tcacccctcg atttggtccc aactctcgga tgtctcattt gtcctctcac
     2521 gagggcaccc gagccgaccg aacaactcct gattggttcg aactggttcg gaggccccgg
     2581 tgtcggcaag cgcaacgatc aagaacaagc tgaagacctg gcaggccggc gcgggcgtca
     2641 tggccctcgg cgcggtgctg ctcggtgccg gtctcctcgt gacgacgtca gccgcgccgc
     2701 ccgcggactg cccggagggc agcacgctcg tctcgaagta caaggccaac aacggcacct
     2761 acacgttcga gaagccggcc gggaacgaca acatcgtcac gctctccaac acgaccgccg
     2821 cgagcggcga ctggagctcg acggtgccga tcgtctacat gatcgtgaag gccgggccgg
     2881 actcgatcac cacgtcgttc agccccgcgc agacgagcgg gtcgtgggac aacctgggct
     2941 tcctcgtcgg cagcggccaa cacccgaaca tctccgacgt gctcttctgc gagtcgacca
     3001 cgcccgcgac gacgacgtcg accgtgacgt cgagcacgac aacgacgcag gccccgacgt
     3061 ccaccacgac gacggccgcg acaacgacgt cgacggcggc gccgaccacg acgagcacgt
     3121 tgccgtcggt gacgacgtcg accggcgcgg cgacgaccac gacgacggtc gcgcccacga
     3181 ccacggcaac cgcggcaccc gtgacgacga cgactgtgcc gatcgtcgtc gagggtacga
     3241 cgacgaccgt tgcggcggcc acgtcgacga ccgggccggc ggggtccacg acgaccgttg
     3301 cgaacgccgt cgtcacgacg accacgacgg atccgagcca gaccgggtcg aactcggccg
     3361 tggtgacgac gacctcgatc gggcactcgg acacgtcgtc ggagtcgctg ccacggaccg
     3421 gtggccagtc gacgccgctc gtcgtgaccg gcctgatctt cctggtcgcc ggcttggcga
     3481 tgacgctgct cgcactgaac cggcgccgga cggcgtaacg caccggcgaa cgaactgaca
     3541 ccgcgatgac acgcggccgg gcgatcgacc tcgcggtcgc cgcgctctcc acgggcgcgg
     3601 cgatctggtc gatggtcgtg gtcgacgtcg actcgatcgt cccggcgacg tgcgtcgt
//
LOCUS       DASUGA010000456         2805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_981660, whole genome shotgun sequence.
ACCESSION   DASUGA010000456 DASUGA010000000
VERSION     DASUGA010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981660"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..752
                     /locus_tag="VFX21_09050"
     CDS             186..752
                     /locus_tag="VFX21_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096148.1"
                     /translation="MFRRRRDQPPVPPPIDHLPRRWREPVAQAIAARDRFAATVRTTR
                     PGPLQEQLETMARDLDAGVAYARSTAEAAYAAEVSLDALDVDDVGTRLKRARRDQSVL
                     PDGSAERIRADETVALLAEQFATLNRVHNRLDDTAAELSRLELRLETAVARAIELTFT
                     PGEGADSGIADVVADLAALQSALDDLRA"
     gene            818..2344
                     /locus_tag="VFX21_09055"
     CDS             818..2344
                     /locus_tag="VFX21_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011896245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HEX5096149.1"
                     /translation="MQGTSDPNRELLDAAALCGHLVAAGSVHAFLAEHRGRLFPDEMF
                     ADLFGSGRGRPSVPADVIATVMVLQSLEGLSDRDAIGQLRTNIAWKIAAGVSLADEGF
                     HPTVLTLWRNRLRESEHPERIFDAVRAVVAETGVLAGKTRRALDSTVLDDAVVRQDAI
                     MQFVAQIRRVRRVIPAAKALTLTAHDYDNDPGKPLCAWDDRVAIDAVVTALVHDARTV
                     LDALDGADLDDRQAEAVGLLALVAGQDVEPGDQDGTWRIAKRTASDRIVSVVDPESRH
                     VHKTVHNYRDGFKAHLAVEPDTGIVTACDLTAGNVGDGQAAAWLLAVETEPLEVLGDS
                     AYGTGKFRAFLAGADHVAQIKPPPLSRAIEGGFTIDDFEIDLAARTATCPADVTVTIS
                     AKRHAVFGVQCNGCVLRDRCTRAVNGKKLKIHEHHELLAAARRHAQTDEFIENYRQHR
                     PMVERTIAWLVRRHRKVRFRGIARNRIGFAHRCAAVNLRRLLNLGLDHDGERWIIATT
                     "
BASE COUNT          464 a          939 c          947 g          455 t
ORIGIN      
        1 agctcgacgg tacgagggtc gtcagcggtt gcggagaggc gaaggtccct ttctcgcgtg
       61 atgtcgcggc gacccaggta ccggccctgt cccccgtctc gcgcactccg aacagggcgt
      121 tgcgtcgtcg cggtcacact cgcggcgatc ccgcttgcgc ccgatcgcct cctaggctcg
      181 cccggatgtt ccggcggcgc cgcgaccaac ccccggttcc cccgcccatc gaccacctgc
      241 cccgccgctg gcgcgagccg gtcgcccagg cgatcgcggc ccgcgaccgc ttcgccgcaa
      301 ccgtgcgcac gacccgcccg gggccgttgc aggaacagct cgaaacaatg gcccgcgacc
      361 tcgatgccgg cgtcgcatac gcgcgcagca cggccgaagc agcctacgcg gccgaggtga
      421 gtctcgacgc gctcgacgtc gacgacgtcg gcacgcggct caagcgcgcc cggcgtgacc
      481 aatcggtgct ccccgacggc agcgccgaac gcatccgcgc cgacgagacg gtcgcgctgt
      541 tggcggagca gttcgcgacc ttgaaccgcg tgcacaaccg gctggacgac accgccgccg
      601 agctctcgcg actcgagctg cgcctggaga ctgcggtcgc acgcgccatc gagctcacct
      661 tcacgcccgg cgaaggcgcg gactcgggca tcgccgacgt cgtcgccgac ctcgccgcgc
      721 tccaaagcgc gctcgacgac ctccgcgcct aggagactgc tgagtaattc gggtggtggt
      781 gctcgcgatt tggtcgcggg tcggtcacgc tggtcgtatg cagggcacga gcgatccgaa
      841 ccgtgagctt ctcgacgcgg cagcgctgtg cgggcatctt gtcgcggcgg gttcggtgca
      901 cgcgtttttg gccgagcatc gtggtcgcct gttcccggac gagatgttcg cggatctgtt
      961 cgggtcgggt cggggccggc cgtcggtccc ggccgacgtg atcgcgacgg tgatggtcct
     1021 gcagtcgttg gagggcttgt cggatcgcga tgcgatcggc cagctgcgca cgaacattgc
     1081 gtggaagatc gcggcgggtg tgtcgctggc cgatgagggg ttccatccga cggtgttgac
     1141 gttgtggcgc aaccggcttc gcgagagtga gcatccggag cggatctttg atgcggtgcg
     1201 tgcggtggtc gccgagacgg gcgtgttggc gggcaagacg cgccgggcgt tggactcgac
     1261 cgtgttggac gacgcggtgg tgcgtcagga cgcgatcatg cagttcgtcg cgcagatccg
     1321 tcgggtccga cgcgtgatcc ccgccgcgaa agcgctgacg ttgacggcgc acgattacga
     1381 caacgatccg ggcaagccgc tgtgcgcgtg ggacgaccgt gtggcgatcg acgcggtcgt
     1441 gaccgcgctt gtgcacgacg cgcgcacggt gctcgatgcg ctcgacggtg ccgacctcga
     1501 cgaccgacag gccgaggccg tcggattgtt ggcgttggtt gccggtcaag acgtcgagcc
     1561 cggcgatcaa gacggcacct ggcggatcgc gaaacgcacg gcttccgatc ggatcgtgtc
     1621 agtggttgat cccgagtcac gccacgtgca caagaccgtg cacaactacc gcgacggctt
     1681 caaggcccat ctcgcggtcg aacccgacac tgggatcgtc accgcgtgtg acctcactgc
     1741 tggcaacgtc ggcgacggtc aagccgcagc ctggctgctc gcagtcgaga ccgaaccgct
     1801 cgaagttctc ggcgactccg cctacggcac gggcaagttc cgagcatttc tcgccggggc
     1861 ggatcacgtc gcacagatca agccgccgcc gctgagtcgc gccatcgagg gcggcttcac
     1921 catcgacgac ttcgagatcg atctcgcggc gcggaccgcg acctgcccag cggatgtgac
     1981 cgtgacgatc agcgcgaagc gtcacgctgt tttcggcgtg cagtgcaatg gatgcgtctt
     2041 acgcgatcgt tgcacgcgcg ccgtgaacgg caagaagctc aagatccacg aacatcatga
     2101 gctcctcgca gccgcgcggc gacacgccca gaccgacgag ttcatcgaga actaccgcca
     2161 gcatcgaccg atggttgaac gcaccatcgc ctggctcgtg cgccgacacc gcaaagttcg
     2221 attccgcggc atcgcacgca accgcatcgg tttcgcgcat cggtgcgccg cggtgaacct
     2281 ccgacgcctg ttgaacctcg gcctcgacca cgacggcgaa cgctggatca tcgcaacgac
     2341 ctgagccaat gggcccggga aggcaatgag ctacgtggcc ggagccttcc agcgctgccg
     2401 acttgcaccc atccacgccg gctgttcgga tcggccattt cccggaccca ccgacccaca
     2461 ccaacgctac gacccgacca caccctccga aaaggcgttg ctcagcagtc tcctaacgag
     2521 gcatgaagcc ggcgccgcca gcgagagcga cagcgaacag ggcggtggtg gcgactgcga
     2581 tgcgggtggc gatgcgcttc atggtgttct cccttggtgg tgccgcccca gcggcgacaa
     2641 gagagagcat ccgggaattc ggacagcgcc aacattgaac gggtggctag tccccgaagc
     2701 gcgcgcggaa gaacactgtg agtagtcaga tccacccttg tcagtgtgtg tgagaatgtc
     2761 ttagggtgct gcacgtgggg agattgtccg gagctgccgg gttcg
//
LOCUS       DASUGA010000457         3011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1769793, whole genome shotgun sequence.
ACCESSION   DASUGA010000457 DASUGA010000000
VERSION     DASUGA010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1769793"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VFX21_09060"
     CDS             complement(<1..630)
                     /locus_tag="VFX21_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006380182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5096150.1"
                     /translation="MDLSFSPDEERFRSECRAWLEANVPRGLPSGDTREGFALHLEWE
                     RKLFDARYAVVSWPEEYGGRDASLWEWLIFEEEYYRAGAPQRVTQNGIFLLAPTIFEF
                     GTDEQKARILRAMASGDQTWCQGWSEPNAGSDLAGIQSRATKVDGGWKLNGQKTWTTR
                     GAFCTHLFGLFRSDPEAERHKGLTYFLVDLSTPGVTVRGVERLDGDEGFA"
     gene            complement(643..1218)
                     /locus_tag="VFX21_09065"
     CDS             complement(643..1218)
                     /locus_tag="VFX21_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005167989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5096151.1"
                     /translation="MPGTPPPVTDERDNLLTWLDQQRTMLKVTAYGLTDEQARTPAPP
                     TELTVGGLIKHVAQVEDYWIKIVLQETTGGNDEYEGGLTMRADETLASIIEYYDDVAA
                     HTDKVVAGIADLGQAVPVPRDAPWFPPDIDAWSVRWVLLHIIQETSRHAGHADLIRER
                     VDGATAFPLLAAAEGWPETEWLKPWSPAAGA"
     gene            1362..2306
                     /locus_tag="VFX21_09070"
     CDS             1362..2306
                     /locus_tag="VFX21_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006139873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEX5096152.1"
                     /translation="MRKLVFVTYPGITALDLVGPNEVLGASGGYDISIAALAAGPLRT
                     TRGPGIVAERALASIRGPIDTMIVVGGEGAIEAAKDDALVAAVATLARRSRRVASVCT
                     GSFLLAAAGLLDGKRVTTHWVSCDHLARKFPNVIVDPDPIFVRDGDVWTSAGVTAGMD
                     LALAMVAEDLGQEVALKVARQLVMYVQRPGGQSQFSAQLENQAERDLLRELQAYVAEH
                     PDEDHSVERLAARVAMSPRNFARVFRADVGCTPAVYVERTRVEVARRLLETTSLPVDE
                     IGRAAGFGTPETLRRAFARRVGTTPTEYRDRFRTPIHR"
     gene            2318..2950
                     /locus_tag="VFX21_09075"
     CDS             2318..2950
                     /locus_tag="VFX21_09075"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007389054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX5096153.1"
                     /translation="MDIACFIFDGITALDIVGPYEILQRLPDANVKFVGKTAGPIRTD
                     NQFLALHADYGFADVKSADILVVPGGFATRGLETDTETLDWIRAIDQTTTWTTSVCTG
                     SMLLAAAGLLDGKEATSHWASLDRLKEYGAIPTGKRVVEQGKIITAAGVSSGIDMGLT
                     LAAKIAGDDFAQCVQLGIEYDPQPPFDSGSLDTAPANVVEIMVGMYAARL"
BASE COUNT          478 a         1006 c         1022 g          505 t
ORIGIN      
        1 cgcgaagccc tcgtcgccat ccaatcgttc gacgccgcgc accgtgacgc cgggcgtcga
       61 gagatcgacg agaaagtacg tgagtccctt gtggcgctcg gcttccggat cactacggaa
      121 caacccgaag agatgcgtgc agaacgcgcc gcgggtcgtc caggtctttt ggccgttcag
      181 tttccaaccg ccgtcgacct tcgtcgcgcg cgattggatg ccggcgaggt cgctgcccgc
      241 gttcggttcg gaccagcctt ggcaccacgt ctgatcgccg ctggccatcg cgcgcagaat
      301 gcgcgccttc tgctcgtcgg taccgaactc gaagatcgtc ggcgcgagca agaagatgcc
      361 gttctgcgtg acgcgctgcg gcgcaccggc gcggtagtac tcctcctcga agatgagcca
      421 ctcccacagc gaggcatcgc ggccgccgta ctcttcgggc cacgaaacaa ctgcgtaacg
      481 cgcgtcgaag agctttcgct cccattcgag atggagtgcg aagccttcac gtgtgtcgcc
      541 cgacggcagg ccgcgcggca cattcgcttc gagccacgcg cggcactcgc tacgaaatcg
      601 ttcctcgtcg ggactgaagg agagatccag agggcgcgga tgttacgcgc ctgcggccgg
      661 agaccacggc ttgagccact cggtctcggg ccagccttcg gccgccgcca acaacgggaa
      721 ggcggtcgcg ccgtcgacgc gttcgcggat gagatcggcg tgtccggcgt ggcgcgacgt
      781 ttcctggatg atgtgcagca gcacccaccg caccgaccac gcgtcaatgt cgggcgggaa
      841 ccagggcgcg tcccggggaa ccggcacggc ctggccgaga tcggcgatac cggcgacgac
      901 cttgtcggtg tgcgccgcga cgtcgtcgta gtactcgatg atcgacgcga gcgtctcgtc
      961 cgcgcgcatc gtcaggccgc cctcgtactc gtcgttgccg ccggtggtct cctgcaggac
     1021 gatcttgatc cagtagtcct cgacctgcgc gacgtgcttg atcagtccgc caaccgtgag
     1081 ctcggtgggc ggcgcgggcg tgcgggcctg ctcgtcggta aggccgtagg cggtcacctt
     1141 cagcatggtg cgctgttggt cgagccaggt caggaggttg tcgcgttcgt ccgtgacggg
     1201 cggcggagtt ccgggcatcg gcgggtcctt tcggcgcagg cgattctgtt gcacgattct
     1261 ccccggggaa actggccagc gagtggccac tttcaggaca tggcaggaat cgtcggttcc
     1321 ttgacattgc tgccagcggc tgcggaccgc acaatgacgt cgtgcggaag ctcgtgttcg
     1381 tcacgtatcc cggaatcacg gccctcgacc tggtgggccc gaacgaggtc ctcggcgcat
     1441 ccggcggtta cgacatcagc attgccgcac tcgccgccgg cccgttgcgc acgacgcgcg
     1501 gcccgggaat cgtggccgag cgcgcgctgg cgtcgatccg cggtccgatc gacacgatga
     1561 tcgtcgtggg cggcgagggc gcgatcgagg ccgccaagga cgacgcgctg gtggcggcgg
     1621 tcgcgacgct ggcgcgccgg agccggcgcg tcgcatcggt ctgcacgggc tcgttcctgc
     1681 tcgcggcggc cggcctcctc gacggaaagc gagtgacgac gcactgggtc tcgtgcgatc
     1741 acctcgcgcg gaagtttccg aacgtgatcg tcgatcccga tccgatcttc gttcgtgacg
     1801 gtgacgtgtg gacgtcggcg ggcgtgactg ccgggatgga ccttgcgctc gcgatggtcg
     1861 ccgaagatct cggccaggag gtcgcgctca aggtggcgcg tcaactcgtg atgtacgtcc
     1921 agcgccccgg tggccaatcg cagttcagcg cgcagctcga gaaccaagcc gaacgcgacc
     1981 tgctacgcga gctgcaggcc tacgtcgccg agcatcccga cgaagaccac tcggtcgagc
     2041 gcctcgccgc gcgcgtcgcg atgagccccc gcaacttcgc gcgcgtgttt cgtgccgacg
     2101 tcggctgtac ccccgcggtc tacgtcgagc gcacgcgcgt cgaggttgcg cggcggctgc
     2161 tcgagacgac gagcttgccg gtcgacgaga tcggccgggc ggccggcttc ggcacgcccg
     2221 aaacgttgcg gcgcgcgttt gcccgccggg tcggcacgac gccgaccgag taccgagacc
     2281 gtttccgtac ccccatccac cgctgaggag cgccaccttg gacatcgcct gtttcatctt
     2341 cgacggcatc actgccctcg acatcgtcgg cccgtacgaa attctgcaac gcctgcccga
     2401 cgcgaacgtg aagttcgtgg ggaagacggc gggaccgatt cgcaccgaca accagttcct
     2461 cgcgttgcac gccgactacg gcttcgccga cgtgaagagc gccgacattc tcgtggtgcc
     2521 gggcggcttc gcgacgcgcg ggctcgagac cgacacggag acgctcgact ggattcgtgc
     2581 gatcgatcag accacgacgt ggacgacgtc ggtgtgcacc ggttcgatgt tgctcgcggc
     2641 cgccggtctg ctcgacggca aggaagcgac aagccactgg gcgtcgctcg accggctgaa
     2701 ggagtacggc gcgatcccga cgggaaagcg cgtcgtcgaa cagggcaaga tcatcaccgc
     2761 ggccggagtg tcgagcggca tcgacatggg gttgacgctc gcagccaaga tcgcgggcga
     2821 cgactttgcg cagtgcgtgc agctcggcat cgagtacgac ccgcagccgc cgttcgactc
     2881 cggcagtctc gacacagccc ccgccaacgt cgtcgagatc atggtcggca tgtacgccgc
     2941 ccggctctag ctgctgacga tcaccgcgga gccgtgtccg aacagacctt ggttgatcga
     3001 gaggccgact c
//
LOCUS       DASUGA010000458         2031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1951449, whole genome shotgun sequence.
ACCESSION   DASUGA010000458 DASUGA010000000
VERSION     DASUGA010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1951449"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1591
                     /gene="carB"
                     /locus_tag="VFX21_09080"
     CDS             <1..1591
                     /gene="carB"
                     /locus_tag="VFX21_09080"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX5096154.1"
                     /translation="NSKAKVVILGSGPNRIGQGLEFDYCCCHAAFALRDAGFETVMIN
                     CNPETVSTDYDTTDRLYFEPLTFEDVMSVIDRENTAGGDVSCLVQFGGQTPLKLSLAL
                     QAAGVRILGTSPDSIDLAEDRRRFAQLLWDLGIPQPASGTATSREEAREAAQKIGFPV
                     VVRPSYVLGGRGMAIVYDMGTLDRYMINAVDAAPEHPVLIDKFVEDAFELDVDCVADD
                     TGAVVIGGIMEHIEEAGIHSGDSSCVVPPYLVAERHLATIRDYTRRIARALKVVGLMN
                     VQYAIKDDVVYVLEVNPRASRTVPYLSKATGVPLAKMAALVMAGKTLSQLGLEEDLEV
                     SGVFVKTPVFPFVRFPGVDSILGPEMKSTGEVMGGATTFGAAFAKAQQSVGQRLPEKG
                     SAFISVNNDDKPNVLPIARNLEDLGFRILATRGTAAYLRAHGIGVEVVFKVNEGRPNI
                     VDHILNNQVQILVNTPLGRESFFDDRAMRRAAMLYNVPCITTLTGASAAVEAIRALRG
                     QTLDVRAIQDYHSEIAARAEG"
     gene            1702..>2031
                     /locus_tag="VFX21_09085"
     CDS             1702..>2031
                     /locus_tag="VFX21_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096155.1"
                     /translation="MRCPAALAAIPLTAGISAGIFLSISFHVALPTALWVAAIVAFAV
                     RFDVATIALLVCGFFTAGMALGRGADVASRQPPLRAIFDQHLEPGGYQIFAIVHGRLR
                     SDAARNPA"
BASE COUNT          334 a          664 c          667 g          366 t
ORIGIN      
        1 aaacagcaag gcgaaggtcg tcatcctcgg aagcgggccg aaccggatcg gtcagggtct
       61 cgagttcgac tactgctgct gtcacgcggc gtttgcgctg cgcgacgcgg gcttcgagac
      121 cgtcatgatc aactgcaacc cggaaacggt ctcgaccgac tacgacacga ccgaccggtt
      181 gtacttcgag ccgttgacct tcgaagacgt catgtcggtg atcgatcgcg agaacacggc
      241 gggcggcgac gtgtcgtgcc ttgtccagtt cggcgggcag acgccgctga agctgtcgct
      301 ggccctgcag gcggccggcg tcaggattct cggcacctct cccgattcca tcgacctcgc
      361 cgaagatcgc cgccgcttcg cgcagttgct gtgggatctc ggcatcccgc agcctgccag
      421 cggaacggcg acgtcgcgcg aagaggcgcg tgaagccgcg cagaagatcg ggttcccggt
      481 cgtcgtgcgg ccgtcctacg tcctgggcgg gcggggcatg gccatcgtct acgacatggg
      541 cacgctcgac cgctacatga tcaacgccgt cgatgcggcg cccgagcacc cggtgctgat
      601 cgacaagttc gtcgaggacg ccttcgagct cgacgtcgac tgcgtggccg acgacaccgg
      661 tgccgtcgtc atcggcggca tcatggagca catcgaggag gcgggcatcc actcgggcga
      721 cagttcctgc gtcgtccccc cgtatctcgt cgccgaacgg catctcgcga ccatccgcga
      781 ctacacgcgc cgtattgccc gcgccctgaa ggtggtcggc ctcatgaacg tgcagtacgc
      841 catcaaggac gacgtggtgt acgtgctcga ggtgaacccg cgcgcgtcgc gaacggtgcc
      901 gtacctgtcg aaagccaccg gcgtgccgct ggcgaagatg gcggcgctcg tgatggccgg
      961 caagacgctt tcgcaactcg ggctcgagga agatctcgag gtgtcgggtg tgttcgtgaa
     1021 gacgccggtc ttcccgttcg tgcgtttccc gggtgtcgac tcgattctcg gacccgagat
     1081 gaaatcgaca ggggaggtga tgggcggcgc gacgacgttt ggcgcggcct tcgccaaggc
     1141 gcagcagtcg gtggggcagc gcctgccgga gaaggggtcg gcgttcatca gcgtcaacaa
     1201 cgacgacaag ccgaacgtcc ttccgatcgc ccggaacctg gaggatctcg gatttcgcat
     1261 cctcgcgacc cgtggcacgg ccgcctatct gcgggcgcat ggtatcggcg tcgaggtggt
     1321 cttcaaggtc aacgaggggc gccccaacat cgtcgatcac atcctgaaca accaggtgca
     1381 gatcctcgtc aacacgccgc tcgggcgaga atcgttcttc gacgaccgcg cgatgcggcg
     1441 cgcggcgatg ctctacaacg tgccgtgcat cacgacgttg accggcgcgt cggccgcggt
     1501 cgaagcgatt cgcgcgctgc gcgggcaaac gctcgacgtg cgcgcgatcc aggactacca
     1561 ctcggagatt gctgcccgcg ccgaaggcta atcgtacgta ctgtacgtag tgtccggctt
     1621 tagccggacc ggccaacgac gagttgtacg taccggacaa cgacgtactg gaataggcac
     1681 cgggtttgct cgcccccgcg cgtgcgttgc ccggccgctc tcgccgccat cccgctgaca
     1741 gccggaattt ccgccggcat tttcctctca atcagctttc atgttgccct gcccacggct
     1801 ctgtgggtgg cggccattgt cgcgttcgcg gtgcgcttcg acgtggcaac gattgcactc
     1861 ctggtgtgcg gattcttcac cgctggcatg gcgctcggac gcggtgcaga tgtcgcgtcg
     1921 cggcagcctc cgctgcgcgc aatttttgat cagcacctcg agcctggcgg gtaccagatc
     1981 ttcgcgatcg ttcacggccg cctgcggtcc gacgcggcca ggaacccggc c
//
LOCUS       DASUGA010000459         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1361790, whole genome shotgun sequence.
ACCESSION   DASUGA010000459 DASUGA010000000
VERSION     DASUGA010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361790"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VFX21_09090"
     CDS             <1..582
                     /locus_tag="VFX21_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096156.1"
                     /translation="FNFAAANYSPPPHIFATTAEFDTYVAAHGDDMNGTFYITGGGQN
                     DPVDISGIKVSGDLAIIATQAPITADSGSADVTSAPGSPPDKILVLVSFYAPPENVAC
                     ATNGGNPGDCAIGIKNNFGPGDNTATLVYAPNGPVAFKNKGGSTPDFLGAIYASNIVL
                     KNNQVVEYDSRVEQVVGFGPVTLERESWVEVSD"
     gene            634..1908
                     /gene="hisS"
                     /locus_tag="VFX21_09095"
     CDS             634..1908
                     /gene="hisS"
                     /locus_tag="VFX21_09095"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX5096157.1"
                     /translation="MPDRDVYRAPKGMLDVLPPASARWIDVVTSFATLAQRYGFGLVL
                     TPIVEHYEVFQRVGESTDVVTKEMYEFTDRGGRHLALRPEGTAGVVRAFVQHRPPVPW
                     KVWYVAPHFRAEAPQAGRYRQHWQVGVEVLGVDDAGVDVEVIALADAFYRDVGLKDVH
                     LRVNSMGDSQTLRRYSNALREYWRSHASAIGNEIDRAETNPMRVLDWKRPDLAEVIEH
                     APQFDEFRSDESWAQWTQLQEGLQAIGLPYEVAPRLVRGFDYYRGTVFEFASSSLEAA
                     QNGVGGGGRYDLLAEQMGGPPTPGIGFGIGIERVLLALDSEGAVPVAPGIDVFVVDMV
                     GGTESLRVLQELRALGLSADRAYGGRSVKKQWSAADKAGARYGVMLGARELAEGNVAI
                     KHLGSGEQIDVHRDEVAAWLKTRIDQNRNLLS"
BASE COUNT          338 a          676 c          693 g          302 t
ORIGIN      
        1 ttcaacttcg ccgccgccaa ctacagcccg ccgccgcaca tcttcgcgac gacggccgag
       61 ttcgacacct acgtcgccgc gcacggcgac gacatgaacg gcaccttcta tatcaccggt
      121 ggcggccaga acgatccggt cgacatctcg gggatcaagg tgtcgggtga cctcgccatc
      181 atcgcgacgc aagcgccgat cacggccgac agcggatcgg ccgacgtcac gtccgccccc
      241 gggagcccgc ccgacaagat cctcgtgctc gtgtcgttct acgcgccgcc ggagaacgtc
      301 gcgtgcgcga cgaacggtgg caaccccggt gactgcgcga tcgggatcaa gaacaacttc
      361 ggtccggggg acaacaccgc cacgttggtc tacgcgccca atggccccgt tgcattcaag
      421 aacaagggcg gatccacgcc cgacttcctc ggtgcgatct acgcgagcaa catcgtcctc
      481 aagaacaacc aggtcgttga atacgactcg cgcgtcgagc aggtcgtcgg cttcggcccg
      541 gtgacgctcg agcgggagag ctgggtcgag gtctccgact gacctacgca taaccggcgg
      601 cctggccgcg accggccggt acgatgcgcg gcgatgccgg atcgtgacgt ataccgcgcg
      661 cccaaaggaa tgctcgacgt cctcccgcca gcgtccgcgc gctggatcga cgtcgtcacc
      721 agcttcgcga cgctggcgca gcgctacggc ttcgggttgg tgctgacgcc gatcgtcgag
      781 cactacgagg tgttccagcg cgtcggcgag tcgaccgacg tcgtcaccaa ggagatgtac
      841 gagttcaccg atcgcggtgg gcgccacctc gcgctgcgac cggagggcac ggcgggggtc
      901 gtgcgcgcgt tcgtgcagca ccggccgccg gtgccgtgga aggtgtggta cgtcgcgccg
      961 cacttccggg ccgaagcacc gcaggccggg cgctaccgcc agcattggca ggtcggcgtc
     1021 gaagtgctcg gtgtcgacga tgccggcgtc gacgtcgagg tcatcgcgct cgccgacgcc
     1081 ttctaccgcg acgtcggatt gaaggacgtg cacctgcgcg tcaactcgat gggagactcc
     1141 cagacgctcc ggcggtattc gaatgcgctg cgcgagtact ggcggtcgca cgcgagtgcg
     1201 atcggcaacg agatcgatcg cgccgagacg aacccgatgc gcgtcctcga ctggaagcgg
     1261 cccgacctcg ccgaggtcat cgagcacgcg ccgcagttcg acgagttccg ctccgacgag
     1321 tcgtgggcgc aatggacgca gctgcaagag gggctgcagg cgatcggctt gccgtacgag
     1381 gtcgcgccgc gcctcgtccg tggattcgac tactaccggg gcaccgtgtt cgagttcgcg
     1441 agcagctcct tggaggccgc gcaaaacggc gtcggcggcg gcgggcgcta cgacctgctg
     1501 gccgagcaga tgggcggacc accgacgccc ggcatcgggt tcggcatcgg catcgagcgc
     1561 gtcctgctcg cgctcgacag cgaaggcgcg gtgccggtcg caccgggcat cgacgtgttc
     1621 gtcgtcgaca tggtcggcgg caccgaatcg ttgcgcgtgt tgcaagagtt gcgcgcgctg
     1681 ggcctgtccg ccgatcgcgc gtacggcggc cgttcggtga agaagcagtg gtcggccgcg
     1741 gacaaggccg gcgcccgcta cggggtgatg ctgggcgccc gcgagctcgc agagggcaac
     1801 gtcgcgatca agcacctcgg cagcggtgag cagatcgacg tccaccgcga cgaggtcgca
     1861 gcctggctca agacgagaat cgaccaaaac cgcaacctgc tcagctagcc ccggtcgcgc
     1921 gtacctgcgt ccctgaacca ccgggacggt ggtcaggtca cgcaggtacg ggttctcgta
     1981 gcctgcgggg gtggcgaagg ggcccgatc
//
LOCUS       DASUGA010000460         2472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_923462, whole genome shotgun sequence.
ACCESSION   DASUGA010000460 DASUGA010000000
VERSION     DASUGA010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923462"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VFX21_09100"
     CDS             complement(<1..515)
                     /locus_tag="VFX21_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018636442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5096158.1"
                     /translation="MSADSGGVTATRYVGARIPRIEDGRLLTGRSSYVDDIVLPRMLH
                     ARFVRSTFARAAIRSIDTSAALAIPGVRFVFTAADVNAGAQAQWHTSIGPHGPETPRP
                     PLAEGEVRFVGDPVALVVADSLAIAHDAAELVEIDYDPLPVVVDYRTAADADALVHEA
                     HGSNVAAEMSGL"
     gene            complement(512..1252)
                     /locus_tag="VFX21_09105"
     CDS             complement(512..1252)
                     /locus_tag="VFX21_09105"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5096159.1"
                     /translation="MGELDGKVAVITGAGSGMAKASVQAFVREGAKVIAADISGAEKD
                     TAAEVGEGVVPFHCDVTNEADMEAVMRAAVEEFGRLDAVCNVAGIGDAAMVADITMEH
                     YDRVMDIDLRGVVLGTKHGIRTMLECGNGGAIVNWSSIGGLNASPFTGVYSAAKAGVI
                     SISKTAAVEYAGKGIRVNTVCPGYIKTEGMGSGLDSYPGATEHTPMKRLGQAPEIAEV
                     AAFLCSDRASFVTGAVIPIDGGRSILIP"
     gene            complement(1361..2248)
                     /locus_tag="VFX21_09110"
     CDS             complement(1361..2248)
                     /locus_tag="VFX21_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013426429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5096160.1"
                     /translation="MSTYQFDGRVAIVTGAGRGLGRAYARLLAERGARVVVNDLGGSI
                     DGAGADDSVAASVATEIGDVALADTNDVSTPEGATALIDAALERFGRIDALINNAGII
                     RWATMPAIDADTVQRHLDVHTLGSFNTTRAAWPHMVEQGYGRVVMTASAGVFGLAGNL
                     AYATAKGAVIGLARTLATEGAAWGIAVNVVAPAAATRMAGEPDATLERVMAPELVAPL
                     VAYLAHESCTANGEIYQAGAGRFARIFLATTEGFVASGTPTVEDVAEHWAAINDETGY
                     AVPSDLTAWSAAFTKHLNR"
     gene            complement(2245..>2472)
                     /locus_tag="VFX21_09115"
     CDS             complement(2245..>2472)
                     /locus_tag="VFX21_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5096161.1"
                     /translation="QFHVSFQDDPIAVACRHITGLSSIVWGNDYPHAEGTFRGSRQLI
                     KEQLGGLPDDERRALVGGTLGNLLGLSPVAN"
BASE COUNT          378 a          856 c          834 g          404 t
ORIGIN      
        1 aggccgctca tctcggccgc gacgttcgag ccgtgcgctt cgtgcacgag cgcgtccgca
       61 tcggccgcgg tccggtagtc gacgacgacc ggcagcgggt catagtcgat ctcgaccaac
      121 tcggccgcgt cgtgcgcgat cgcgagtgag tcggcgacga ccagcgctac cggatcaccg
      181 acgaagcgga cctcgccctc ggcgagcggc ggtcgcgggg tctccggtcc gtgcgggccg
      241 atcgacgtgt gccactgcgc ctgcgcgccg gcattgacgt ccgcggccgt gaacacgaag
      301 cgcacgcccg ggatcgcgag cgcggcactc gtgtcgatac tgcgaatcgc ggcgcgggcg
      361 aaggtgctgc gcacgaaccg ggcatgcagc atgcggggca gaacgatgtc gtcgacgtac
      421 gaactgcgac cggtcagcag ccggccatcc tcgatgcgcg ggatgcgcgc gccgacgtac
      481 cgggtggcgg tgacgccgcc agaatccgcg ctcacggaat caggatgctg cggccgccgt
      541 cgatcggaat caccgcgccg gtcacgaacg acgcgcggtc cgagcacaag aacgccgcga
      601 cttccgcgat ctcgggcgcc tgcccgagcc gcttcatcgg cgtgtgttcc gtcgcgcccg
      661 gatagctgtc gagtcccgaa cccatgcctt cggtcttgat gtagccgggg cacacagtgt
      721 tcacgcgaat gcccttgccc gcgtactcaa ccgccgcggt cttggagatc gagatgacac
      781 ccgctttggc ggcggagtac acgccggtga acggcgacgc gttgaggccg ccaatcgacg
      841 accagttcac aatcgcaccg ccgttgccgc actcgagcat ggtgcggatg ccgtgcttgg
      901 tgccgagcac cacgccgcgc aggtcgatgt ccatgacccg gtcgtagtgc tccatggtga
      961 tgtcagcgac catcgcggca tcgccgatgc cggccacgtt gcagaccgca tcgaggcggc
     1021 caaactcctc gaccgccgcg cgcatgacgg cttccatgtc ggcttcgttg gtgacgtcgc
     1081 agtggaaggg cacgaccccc tcgccgactt cggccgcggt gtctttctcc gcgccgctga
     1141 tgtcggcggc gatgaccttg gcgccctcgc gcacgaacgc ctgcaccgat gccttggcca
     1201 tgcccgaccc cgcgccggtg atgactgcaa ccttgccgtc caactcgccc atgtggcgtt
     1261 ctcccgtggc ttccgatttg gcgccaggag catgccacca aatggggccg ccagcaaggg
     1321 ggcgcttcag gcggggattt tttcccgccc tgaagcgcct ctaacggttg aggtgcttgg
     1381 tgaaggcggc ggaccaggcg gtcaggtcgc tcgggaccgc gtagccggtt tcgtcgttga
     1441 tcgccgccca gtgctcggca acgtcttcga ccgtgggggt cccgctcgcg acgaacccct
     1501 cggtcgtggc gaggaagata cgcgcaaaac ggcccgcgcc ggcttgatag atctcgccgt
     1561 tggcggtgca cgactcgtgc gcgaggtagg cgaccagcgg cgcgacgagt tcgggagcca
     1621 tcactcgttc gagcgtcgca tcgggctcgc ccgccatgcg cgtggccgcc gcgggcgcga
     1681 cgacattgac cgcaatgccc cacgcagcgc cttcggtcgc cagcgtgcgc gccaatccga
     1741 tgactgcacc tttggccgtc gcgtacgcga ggttgcccgc gagtccgaac acgccggccg
     1801 acgcggtcat gacgacgcgg ccgtagccct gttcgaccat gtgcggccat gcggcgcgcg
     1861 tcgtgttgaa cgacccgagc gtgtgcacgt cgagatgccg ttgcaccgtg tccgcgtcga
     1921 tcgcaggcat cgtcgcccag cggatgatgc cggcgttgtt gatgagtgcg tcgatccgac
     1981 cgaatcgctc gagcgcagca tcgatcagcg cggttgcgcc ttcgggcgtc gagacgtcat
     2041 tcgtatcggc gagcgcgacg tcgccaatct cggtcgcgac gctcgcagcg acgctgtcgt
     2101 cggcgccggc gccatctatg gaaccgccga ggtcgttgac gacaacccgc gcgccgcgtt
     2161 ccgcgagcag gcgtgcgtag gcgcggccca agccgcgtcc tgcgccggtg acgatcgcga
     2221 cgcgcccgtc gaactgatac gtgctcagtt ggccaccggg ctcaggccga gcagatttcc
     2281 gagcgtgccg cccacgagcg cgcgccgctc gtcatccggc aagccaccga gctgttcctt
     2341 gatcagctgg cggctccccc ggaacgttcc ctccgcgtgc ggataatcgt tgccccagac
     2401 gatgctcgac agtccggtga tgtggcggca cgcaaccgca atcggatcgt cttggaacga
     2461 cacgtggaac tg
//
LOCUS       DASUGA010000461         3146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_100522, whole genome shotgun sequence.
ACCESSION   DASUGA010000461 DASUGA010000000
VERSION     DASUGA010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100522"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VFX21_09120"
     CDS             <1..348
                     /locus_tag="VFX21_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096162.1"
                     /translation="QYYAAPTHEPVTRRFLTAARFTDPTKTEDAFWEIEWLDVAPGDL
                     AWHEPLVSIVDEAGATVADDQRGRIDVTYLGVTSGGHRYRTRWYDPAFGPAHRFVLAA
                     NAGQPQLASPPFD"
     gene            380..1231
                     /locus_tag="VFX21_09125"
     CDS             380..1231
                     /locus_tag="VFX21_09125"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX5096163.1"
                     /translation="MPVPDKRVTIDEIVSEVRDGMTVGIGGWGSRRKPMALVRALCRS
                     SVKDLKIVSYGGPDVGLLCRAGKVREVAYAFVSLDSIALEPHFRNARQSGSVSTMELD
                     EGMFLLGLQAAAWRVPFLPTRVGLGSDVMRINKTLKTVTSPYEDGEELVAMPALQLDV
                     ALIHMHRGDQGGTGQFLNIDPYFDDLFCGAATKRFMSVEKLIDTDDFLKEGPVHSIRI
                     NRLLTDGVVETPHGAHFTECVPDYPRDEAFQRAYTASAKSDEAWDEFRVAYIDVTEAE
                     YQQAVSK"
     gene            1270..2001
                     /locus_tag="VFX21_09130"
     CDS             1270..2001
                     /locus_tag="VFX21_09130"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX5096164.1"
                     /translation="MAIADCFAHDGEILANPIGTLPVIGGRLAKATVAPELVMTDGEA
                     VLVENILPVGAAAPEKVVSGWNPYRSMFDVVWSGRRHVMMGGSQFDRHGNQNFAFIGS
                     HEAPRTQLLGMRGAPGNTINDKTSYWVPQHGTRVFVEAVDVVSGVGYDRAAELGVAER
                     RFHELGQVITNLCVMDFASADRRMRLRSVHPGVTVDDVVAATGFELVIDGDVPESRLP
                     TDDELRWIREVIDPDGLGEQEVPNP"
     gene            2004..3053
                     /locus_tag="VFX21_09135"
     CDS             2004..3053
                     /locus_tag="VFX21_09135"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEX5096165.1"
                     /translation="MRTPLCDLFGIDVPIVQTGMGWVAGARLVTATANAGALGILASA
                     TMTYDQLADAIREVKDRTDKPFGVNLRADAEDADARVQLLIKEHVKVASFALAPSEVQ
                     IKTLKDAGVRVVPSIGAKRHAEKVAAWGVDAVLVQGGEGGGHTGQVATTLLLPQVIDA
                     VDLPVIAGGGFFDGRGLVAALAYGAAGIAMGTRFLLTQESTVPDAVKQYYLGRAVTDT
                     VVTAKIDGVPHRVLRTEFVEEIDKGSPVTAYPRAARNALAFKKMSGISWKGMLASGRE
                     MKHEGNLKWSQVMLAANTPMLLRAGLVEGNLDAGVLSSGQAAGVIDDLPTVAELVSRI
                     MREADETIARLADLA"
BASE COUNT          531 a         1035 c         1072 g          508 t
ORIGIN      
        1 cagtactacg ccgcgccgac ccacgagccg gtcacgcgcc ggttcctcac cgccgctcgc
       61 ttcaccgacc ccaccaagac cgaagacgcg ttttgggaga tcgagtggct cgacgtcgcg
      121 cccggcgatc ttgcctggca cgagccgctc gtctcgatcg tcgacgaggc gggtgcaacc
      181 gtcgccgacg accaacgcgg ccgcatcgac gtgacgtacc tcggagtaac cagcggcgga
      241 caccggtatc gcacgcgttg gtacgacccc gcgttcggtc cggcacaccg cttcgtgctc
      301 gccgccaacg ccggtcaacc gcaactcgcg tcgcctccct tcgactagag aggccttcga
      361 ctaggtaacg tcccgaaccg tgccggttcc cgacaagcga gtcaccatcg acgagatcgt
      421 gagcgaggtg cgcgacggca tgaccgtcgg catcggcggg tgggggtcgc ggcgcaagcc
      481 gatggcgctc gtgcgcgcgc tctgccgttc gtcggtcaaa gacctgaaga tcgtttccta
      541 cggcgggccc gacgtcgggc tgttgtgccg ggcgggcaag gtgcgcgaag tggcgtacgc
      601 gttcgtgtcg ctcgactcga ttgcgctcga gccgcacttc cggaacgcgc gtcagagcgg
      661 gagcgtgtcc acgatggaac tcgacgaagg catgttcctg ctcggcctcc aggccgcggc
      721 gtggcgggtg ccgttcctgc ccacccgcgt cggactcggc tccgacgtga tgcgcatcaa
      781 caagacgctg aagacagtga cttcgccgta tgaggatggt gaagaacttg tcgcgatgcc
      841 tgccctgcag ctcgacgtcg cgttgattca tatgcaccgc ggcgaccagg gcggcaccgg
      901 ccagttcctg aacatcgatc cctacttcga cgatctcttc tgcggcgccg caacgaagcg
      961 gttcatgagc gtcgagaagc tgatcgacac cgacgacttt ctcaaagagg gcccggtgca
     1021 ctcgatccgc atcaaccgct tgctgaccga cggcgtggtc gagacgccgc acggcgcgca
     1081 cttcaccgag tgcgtccccg actacccgcg cgacgaagcg tttcaacggg cgtacaccgc
     1141 gtcggcaaag tccgatgagg cgtgggacga gtttcgcgtg gcctacatcg acgtgaccga
     1201 agccgaatac cagcaggcgg tctcgaaatg acgcgcgacg gagcgcccac gcgggccgaa
     1261 gtatgcgcgg tggcgatcgc cgactgtttc gcgcacgacg gcgagatcct cgccaacccg
     1321 atcggcacgt tgccggtgat cggcggccgg ctcgcgaaag cgaccgtcgc gcccgagctg
     1381 gtgatgaccg acggcgaagc ggtgctggtc gagaacattc tgccggtcgg cgccgccgcg
     1441 ccggagaagg tcgtgagcgg ctggaacccg taccggtcga tgttcgacgt ggtgtggtcg
     1501 ggccggcggc acgtgatgat gggcggcagc cagttcgatc gccacggcaa tcagaacttt
     1561 gcgttcatcg gctcgcacga ggcaccccgt acgcagctgc tcgggatgcg cggggctccg
     1621 ggcaacacga tcaacgacaa gacgtcgtat tgggtgccgc agcacggcac gcgcgtcttc
     1681 gtcgaagctg tcgatgtggt gagcggcgtc ggctacgacc gcgccgccga actcggcgtc
     1741 gcagagcggc gcttccacga acttggccag gtcatcacca acctgtgcgt gatggatttc
     1801 gcctcggccg accgccgcat gcggctgcgc tcggtgcacc cgggtgtcac cgttgacgac
     1861 gtcgtcgcgg cgaccggttt cgagttggtg atcgacggtg acgttcccga gtcacggctg
     1921 ccgaccgacg acgaactgcg ctggatccgc gaggtcatcg accccgacgg gctcggcgag
     1981 caagaagttc cgaacccgtg acgattcgca cgcctctgtg cgacctcttc ggcatcgacg
     2041 tgccgatcgt gcagaccggc atgggttggg tggccggggc gcggctcgtc accgccacgg
     2101 caaatgccgg cgcgctcggc attctcgcca gcgcgacaat gacgtacgac cagctcgcgg
     2161 acgcgattcg cgaggtgaag gatcggaccg acaagccgtt cggcgtaaac ctgcgcgccg
     2221 atgccgaaga cgctgacgct cgcgtgcagc tgctcatcaa agagcacgtg aaagtcgcgt
     2281 cgttcgcgct cgcgccgagt gaggtccaga tcaagacgtt gaaggacgcg ggcgtgcgcg
     2341 tcgtgccgtc gatcggcgcc aagcgccatg ccgagaaggt cgcggcgtgg ggcgtcgatg
     2401 cggtgttggt gcaaggcggc gaaggtggcg gccacaccgg ccaggtcgca accacgttgc
     2461 tgttgccgca ggtcatcgac gccgtagacc tgccggtcat cgcgggcggc ggcttcttcg
     2521 acgggcgtgg cctcgtagcc gccctggcgt acggcgcagc gggtatcgcg atgggaacgc
     2581 gctttctgct cacgcaggag agcacggtgc ccgacgcggt caagcagtac tacctcggcc
     2641 gagcggtgac cgacacggtc gtgacggcca agatcgacgg tgtgccgcac cgcgtgttgc
     2701 gcacggagtt cgtcgaggaa atcgacaagg gttcaccggt cactgcgtat ccccgcgccg
     2761 cgcgcaatgc gctggccttc aagaagatgt cggggatctc ctggaagggg atgctcgcct
     2821 cgggccgcga gatgaagcac gagggcaacc tgaagtggtc gcaggtcatg ctggccgcga
     2881 acacgccgat gttgctgcga gccgggctcg tcgaaggcaa tctcgacgcg ggcgtgctct
     2941 cgagcgggca ggccgcgggc gtgatcgacg acctcccgac cgtcgcggag ctcgtctcac
     3001 gcatcatgcg cgaagccgac gagacgatcg ctcgcctcgc cgacctcgcg tagacctcgc
     3061 tagggcaacg tcgcctgcgg ccgggtgagg tactccttca ccgtccagta cttgtcccgg
     3121 ggcacccccg tcttcgggtc gaccaa
//
LOCUS       DASUGA010000462         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1568487, whole genome shotgun sequence.
ACCESSION   DASUGA010000462 DASUGA010000000
VERSION     DASUGA010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568487"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="VFX21_09140"
     CDS             complement(<1..1127)
                     /locus_tag="VFX21_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEX5096166.1"
                     /translation="MNPLAPILLAKTLAADPLFSNGIDTEVVIIVIVKTILVFVILLV
                     GVMFYVWFMRKVIADMQNRIGPQRAGPFGAFQTLADGIKLFFKEQSTPTSADRPVFRL
                     APYLAIMPAFLAFAIVPIGGLVTVDGHHTYLQLADPGIGVLWLLMMSGIGLYGVMLAG
                     WSSGSKYPLLGSVRASAQLLSYEAALGLAVVGVLVHSGTLSTRAIVDQQAWTSWDSFA
                     TEWYWLPAIGAFVIFVIAAIAETNHPPFDLVEAEQELVGGFHTEYTGIRFAIFFLAEF
                     MNLITMCAIAATLFLGGPSGPALGFISADSWINVWVMPVLYFVVKVLVLLYATVWVRA
                     TLPRLRYDQLMSLGWKYLIELAFIWAMVSAVVVVGADQGWNR"
     gene            complement(1124..>2084)
                     /locus_tag="VFX21_09145"
     CDS             complement(1124..>2084)
                     /locus_tag="VFX21_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX5096167.1"
                     /translation="LGSDPLNDFPDRALAAHALDAVERVIVVGAVPADGGNKTSVVLP
                     VTVWGEQEGTTSNLEGRVQRVARKVTPGGTAMEGWRIAGELATRFDADFDLETTAEVQ
                     DEIARVAPAFAGVDAQLLRSARDGVVLPLADHADEIEFGPAPLGAGVSWEPIPSGSDA
                     EATTSADVEVAATDAEVDTEAEAAAAAEPPTPTLPLHVWHGSAPAAAVVPVDGYALRL
                     VAARTLYGTDRAVFESPALATFAPTDPVLLVHRVDRDRIGVVDGDRVRITSARGTVEL
                     PIVADASTQQGTAFLAVNRQGPGASELVDPNEPVTDLRVETVR"
BASE COUNT          361 a          702 c          745 g          276 t
ORIGIN      
        1 cggttccaac cctggtcggc gccgacgacg accaccgccg aaaccatcgc ccagatgaac
       61 gcgagctcga tgaggtactt ccacccgagc gacatgagct ggtcgtagcg gagacgggga
      121 agcgtggccc gcacccacac cgtcgcgtac aggagcacga gcaccttcac gacgaagtac
      181 aggaccggca tcacccagac gttgatccag ctgtcggccg agatgaaccc gagagccgga
      241 cccgacggcc cgccgaggaa cagcgtcgcc gcgatcgcgc acatcgtgat gaggttcatg
      301 aactcggcga ggaagaagat cgcgaaccgg atgcccgtgt actcggtgtg gaatccgccg
      361 accagctcct gctcggcctc gacgaggtcg aacggcgggt ggttcgtctc ggcgatcgcg
      421 gcgatgacga agatcacgaa cgcgccgatc gcgggaagcc agtaccactc cgtcgcgaac
      481 gagtcccagc tcgtccacgc ctgctggtcg acgatggccc gagtcgacag cgtccccgag
      541 tgcacgagga cgccgacgac ggcgaggccg agcgcggctt cgtagctgag cagctgcgcg
      601 gacgcgcgta cggaaccgag caacgggtac ttcgagcccg acgaccagcc ggcgagcatc
      661 accccgtaga gcccgatgcc cgacatcatc agcagccaga gcacgccgat gccggggtcg
      721 gcgagctgga ggtaggtgtg gtggccgtcg acagtgacga gaccgccgat cggcacgatc
      781 gcgaacgcga ggaacgcggg catgatcgcg aggtagggcg cgagccggaa gaccggtcgg
      841 tcggccgacg tcggcgtgga ctgctccttg aagaagagct tgatcccgtc ggccagggtc
      901 tggaacgcgc cgaacggccc tgcgcgctgc gggccgatgc ggttctgcat gtcggcgatg
      961 accttgcgca tgaaccacac gtagaacatc acgccgacga gcaggatcac gaagacgagg
     1021 atcgtcttca cgatgacgat gatcacgacc tcggtgtcga tgccgttcga gaacagcggg
     1081 tcggccgcaa gggtcttcgc gaggaggatc ggagccagcg ggttcatcgc acggtctcca
     1141 cgcgcagatc ggtgaccggc tcgttcggat cgacgagctc gctcgcgccg ggaccctgac
     1201 ggttcaccgc gaggaacgcc gtgccctgtt gcgtggaggc gtcggccacg atcggcagct
     1261 cgaccgtgcc ccgcgccgac gtgatgcgca cgcggtcgcc gtcgacgacg ccgatgcgat
     1321 cgcggtcgac tcggtgcacg agcagcacgg ggtcggtggg tgcgaacgtg gcgagggcgg
     1381 gcgactcgaa caccgcgcga tcggtgccgt agagcgtgcg ggccgcaacg aggcggagcg
     1441 cgtagccgtc gacgggaacg acggccgcgg cgggcgccga cccgtgccac acgtgcaacg
     1501 gcagcgttgg cgtgggcggc tcggccgctg ccgcggcttc ggcctctgtg tcgacctcgg
     1561 cgtcggttgc ggcgacctcg acgtcggcgg aggtcgtagc ttcggcgtcg gagccggacg
     1621 ggatcggttc ccacgacact ccggcaccca acggcgccgg accgaactcg atctcgtcgg
     1681 cgtgatcggc gagcgggagc accacgccgt cgcgcgccga ccgcaacagc tgcgcgtcga
     1741 ctccggcgaa cgccggcgcg acgcgcgcga tctcgtcctg cacctcggcg gtggtctcga
     1801 ggtcgaagtc ggcatcgaag cgagtcgcga gctcgcccgc gatccgccac ccctccatcg
     1861 cggtgccgcc cggcgtcacc ttgcgcgcga cacgctgcac gcggccctcg aggttcgacg
     1921 tcgtgccttc ctgctcaccc cacaccgtca cgggaagcac gaccgacgtc ttgttgccgc
     1981 cgtcggcggg gaccgcgccg acgacgatga cgcgctcgac ggcgtcgagc gcgtgcgcgg
     2041 caagcgcgcg gtcggggaag tcgttgagcg ggtcggagcc gagc
//
LOCUS       DASUGA010000463         2527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1419449, whole genome shotgun sequence.
ACCESSION   DASUGA010000463 DASUGA010000000
VERSION     DASUGA010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419449"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="VFX21_09150"
     CDS             complement(<1..1245)
                     /locus_tag="VFX21_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020461226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5096168.1"
                     /translation="MTEFDAIDFFRGNELIDDPYSYFDHLRARCPVTRESHHDVMMVT
                     GYDEAIAVYNDTATYSSCNSVTGPFPGFPVALEGDDVSALIEQHRDALPFSDQLPTFD
                     PPRHTDHRALLMRLITPKRLKENEEFMWRLADRQIDEFIERGSCELIGDYGGPFAMLV
                     VADLLGVPEEDHEDFREQLGGGKRNRTGTVGGTGTAGTKDLEHNPLEFLYERFTEYVE
                     DRRRSPRDDVMTGLATAKFPDGSTPEVIDVVRIAANLFAAGQETTVRLLGTAFQVLGE
                     NPELQADLCANKELVPNFVEEALRIESPVKGDFRLSRVHTTLGGVDIPAGTTIMVVNG
                     AANHDPRRFENPKEFDVNRVNARQHLAFGHGAHTCPGAPLARAEGRVSVERLLDRTTD
                     IRINETEHGPPDARRYEYAPTYI"
     gene            1344..>2527
                     /locus_tag="VFX21_09155"
     CDS             1344..>2527
                     /locus_tag="VFX21_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019926856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5096169.1"
                     /translation="MPRPRTPQPPILLRSARLLDVTRGEYLTPGALLVEGERIVDVAP
                     TSVPDDAVVMDLGDLTLLPGLMDMEVNLLLGGPDHASPLNAVQDDPALRTLRAVANAR
                     RTLRSGFTTVRNLGLFIPTGGLLLDVSLMKAIDFGWIDGPRVVPAGHAICPTGGHLDP
                     TMFQAIAPHLMPLTVEEGIANGIDEVRKAVRYQIKYGAKLIKVCASGGIMSHTGPAGA
                     QQYSNEELAAIADEAHRAGLRVAAHAHGDDGIRAAVGAGIDCIEHCSLATDDTLELMK
                     QRGTFLVATTYLADGMDVSHAAPELQAKAAEVFPQARKTIGRAIEMGVKVACGTDAPA
                     IPHGRNAKELIALVDRGMTPVQAIRAATTVSAELIDQDDCGRLAPGCYADIIGVPGDP
                     TA"
BASE COUNT          386 a          874 c          875 g          392 t
ORIGIN      
        1 gatgtacgtc ggcgcatact cgtagcgtcg cgcgtccggc gggccgtgct cggtctcgtt
       61 gatgcggatg tcggtggtgc ggtcgagcag ccgctccacg ctgacgcggc cctcggcgcg
      121 cgcgagcggc gcacccgggc aggtgtgcgc gccgtggccg aacgcgaggt gctggcgggc
      181 gttgacgcgg ttgacgtcga actccttcgg gttctcgaac cggcgcgggt cgtggttggc
      241 cgcgccgttg acgaccatga tcgtcgtgcc ggccgggatg tcgacgccgc cgagcgtcgt
      301 gtgcacgcgc gacaaccgga agtcgccctt caccgggctc tcgatgcgca gcgcctcttc
      361 cacgaagttg gggacgagct ccttgttggc acacagatct gcctgcagct ccgggttctc
      421 gccgagcacc tggaacgcgg tgcccaacaa ccgcaccgtc gtctcctggc ccgcggcgaa
      481 caggttcgcg gcgatgcgca cgacgtcgat cacctccggc gtcgagccgt cggggaactt
      541 cgcggtcgcg agaccggtca tgacgtcgtc gcgcgggctg cggcgccggt cttcgacgta
      601 ctcggtgaag cgctcgtaca ggaactcgag cgggttgtgc tcgagatcct tggtgccggc
      661 ggtgcccgtc ccgccgacgg tgccggtgcg gttgcgcttc ccgccgccga gctgctcgcg
      721 gaagtcctcg tggtcctctt cgggcactcc gagcaagtcg gcgacgacga gcatcgcgaa
      781 cgggccgccg tagtcgccga tgagctcgca cgaaccgcgc tcgatgaact cgtcgatctg
      841 gcgatcggcg agccgccaca tgaattcctc gttctccttg aggcgcttcg gcgtgatcag
      901 tcgcatcagg agcgcgcggt gatcggtgtg gcgcggcgga tcgaacgtcg gcagctggtc
      961 gctgaacggc agcgcgtcgc ggtgctgctc gatgagcgca ctcacgtcgt cgccttcgag
     1021 cgcgaccggg aaaccgggga acgggcccgt caccgagttg cacgacgaat aggtcgcggt
     1081 gtcgttgtag acggcgatcg cttcgtcgta gccggtcacc atcatcacgt cgtgatgcga
     1141 ctcgcgcgtc accgggcagc gggcgcggag gtgatcgaag tacgagtacg gatcgtcgat
     1201 cagctcgttg ccgcggaaga agtcgatcgc gtcgaactcg gtcacagcgc gggccctcgg
     1261 gatcgggacg agtgattact ccgttcgcat cctaggagag taggatttcc gctgcaacgc
     1321 ccgtggtcac gaacggagcg cccatgccgc gccctcgcac accgcaaccg ccgatcctgc
     1381 tgcggagcgc ccgcctcctc gacgtgacgc gcggcgagta cctcacgccg ggcgcattgc
     1441 tcgtcgaggg cgagcgcatc gtcgacgtcg cgccgacgtc ggtgcccgac gacgcggtcg
     1501 tgatggatct cggcgacctc acgctgctgc ccggcttgat ggacatggaa gtcaacttgc
     1561 tgctcggcgg tcccgaccac gcgagcccgc tcaacgcggt gcaggacgac cccgcgctgc
     1621 gcacgttgcg cgccgtcgcc aacgcgcgcc gcacactgcg ctccggattc acgacggtgc
     1681 gcaacctcgg cctgttcatc ccgaccggcg gactgctcct cgacgtctcg ctgatgaagg
     1741 cgatcgactt cggttggatc gacggcccgc gcgtcgtgcc cgcgggccac gccatctgcc
     1801 cgaccggcgg ccatctcgat ccgacgatgt tccaggcgat cgcgccgcac ctcatgccgc
     1861 tcaccgtcga agaaggcatc gccaacggca tcgacgaagt gcgcaaggcg gtgcgctacc
     1921 agatcaagta cggcgccaag ctgatcaagg tgtgcgcgtc gggcggcatc atgtcgcaca
     1981 ccgggcccgc aggcgcgcag cagtactcca acgaggaact ggccgcgatc gccgacgaag
     2041 cgcaccgtgc cggactgcgg gtcgccgcgc acgcgcacgg cgacgacggc atccgggccg
     2101 cggtcggcgc ggggatcgac tgcatcgagc actgctcgct cgccaccgac gacacgctcg
     2161 aactgatgaa acagcgcggc acgtttctcg tcgcgacgac gtatctcgcc gacggtatgg
     2221 acgtctcgca cgcggcaccc gagctgcagg cgaaggcggc cgaggtcttc ccgcaggcgc
     2281 gcaagacgat cggccgggcg atcgagatgg gtgtcaaggt cgcgtgcggc accgacgcac
     2341 ccgcgatccc gcacggccgc aacgccaagg agctgatcgc gctcgtcgac cgcggcatga
     2401 cgccggtgca agcgatccgc gccgcgacga cggtgtcggc ggagctgatc gaccaggacg
     2461 actgcggccg cctcgcgccc ggctgctacg ccgacatcat cggcgtgccc ggcgacccga
     2521 ccgccga
//
LOCUS       DASUGA010000464         2794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_457335, whole genome shotgun sequence.
ACCESSION   DASUGA010000464 DASUGA010000000
VERSION     DASUGA010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457335"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VFX21_09160"
     CDS             <1..365
                     /locus_tag="VFX21_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096170.1"
                     /translation="VDWMADADSIYFTSGQTRGVGTTFDCVTRIGPFRTVDRMEVTAW
                     DPPRRMGIEHQGLFTGQGRFTLRRTRFGRTRFSWSERIRFPWWLGGPVGALLAKPVFF
                     LVWRGNLRRLRDLVASKT"
     gene            365..1339
                     /locus_tag="VFX21_09165"
     CDS             365..1339
                     /locus_tag="VFX21_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5096171.1"
                     /translation="MNQRAVSIAGVQTLGAPMAVDIAREAGELGYTSVWAAEANAAEA
                     LSVLGAISQVAPTIGLGTGVLALQLRTPPLHAMAAATLQQLAPDRDVFLGIGISSPAV
                     AGQWHGAGYGDRPIAQVREFVTLVRECLSGESVSFDGDFYRVSRFRLGLRLGERKPKI
                     VIAALNPQMLRLAGEIADGVLLNYLPASIVPSCVEQVRKGGDAKIYAFVHAGVTDRDR
                     YADLARKDLVNYAVVDAYANQFARAGYADDVEEFRARWKAKDRDGALAAIGDRWVDEV
                     QIMGDAAHVRAAVQAYADEGVDLPILFALPWGEDRRRTVSQTLQAVAP"
     gene            complement(1336..2118)
                     /locus_tag="VFX21_09170"
     CDS             complement(1336..2118)
                     /locus_tag="VFX21_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012687188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5096172.1"
                     /translation="MTDDAYVLCDRDEHGVALVRLNRPPMNPLSQAMLAALRDVARDL
                     ASDTSVKAVVVAGSEKAFAAGADIDEFGDQAQAQVIERGFRDAFDAVESIPRPVIAAM
                     RGYALGGGLELAMACDLRVASEKARVGQPEILLGVIPGAGGTQRLPALVGPARAKEMI
                     WSGRQVRADEAHAIGLVDKVVHDDEVEHSALHWAAEFAKGAVVAMGLAKRVINGGRGV
                     PLAAALDAETEAFVEVFGTEDARTGVQSFREHGPGKAKFSGR"
     gene            complement(2130..>2794)
                     /locus_tag="VFX21_09175"
     CDS             complement(2130..>2794)
                     /locus_tag="VFX21_09175"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX5096173.1"
                     /translation="TSSPPMADGHPLVRVLADAAHGRFPPNDGVIDVAPAVDGRADAM
                     VGFTGHWVLAADIDPELVHARLPDGDFSAPMSAVFLNWVAAQLGSRPGTFDALLCALG
                     TGDGMPDWLTRVDDLDHPRVARASRYRRDMQIFATADGAGVIILGRGICGRWEIGYEV
                     DASARGEGLGRRLVTAARALVPNGEPVWAQVAPGNAASLRATLAGGFTPVAAEVLFPR
                     RA"
BASE COUNT          404 a          991 c          986 g          413 t
ORIGIN      
        1 acgtcgactg gatggccgac gcggactcga tctacttcac gtcgggacag acgcgcggcg
       61 tcgggacgac gttcgactgc gtgacgcgca tcggcccctt tcgcacggtc gatcgcatgg
      121 aagtcaccgc gtgggacccg ccgcgacgca tggggatcga gcatcaaggt ctcttcaccg
      181 gtcagggccg gttcacgttg cggcgcacgc gattcggtcg cacccgtttc tcctggagcg
      241 agcgcatccg gttcccgtgg tggttgggtg gaccggtcgg cgcgctgctc gcgaagccgg
      301 tgttcttcct cgtgtggcgg ggcaacctgc ggcgactgcg cgaccttgta gcttccaaga
      361 cgtgatgaac cagcgggcag tcagcattgc cggcgtgcag acgttgggcg cgccgatggc
      421 cgtcgacatc gcgcgcgagg cgggcgagct cgggtacacg tcggtctggg ccgcggaggc
      481 gaacgcggcc gaagcactgt cggtgctcgg ggcgatcagc caggtggcgc cgacgatcgg
      541 gctcggcacc ggcgtccttg cgttgcagct ccgtacgccg ccgctgcacg cgatggcggc
      601 cgcgacgctg cagcagctcg cccccgaccg cgacgtcttc ctcggcatcg gcatctcgtc
      661 gccggcagtc gcgggccagt ggcacggcgc gggctacggc gaccggccga tcgcgcaggt
      721 gcgtgagttc gtcacgctgg tacgcgagtg cctgagcggc gagagcgtga gcttcgacgg
      781 cgacttctac cgcgtctcgc gcttccggct cggcctgcgc cttggcgaac gcaagccgaa
      841 gatcgtgatc gcggcgctga acccgcagat gctgcggctc gcaggcgaga tcgcggatgg
      901 cgtgctgctc aactacttgc ccgcgtcgat cgtgccgtcg tgcgtcgagc aggtgcgcaa
      961 gggcggggac gcgaagatct acgcgttcgt gcacgccggc gtcaccgacc gcgaccgcta
     1021 cgccgatctc gcccgcaagg acctcgtgaa ctacgcggtc gtcgacgcgt acgcgaacca
     1081 gttcgcgcgt gccgggtacg cggacgacgt cgaggagttc cgcgcccgct ggaaggccaa
     1141 ggaccgcgac ggggcgctgg ccgcgatcgg cgaccggtgg gtcgacgagg tgcagatcat
     1201 gggtgacgcc gcgcacgttc gcgccgcggt gcaggcgtac gccgacgagg gcgtcgacct
     1261 gccgatcctc ttcgcgctcc cctggggcga ggaccgccgc cggacggtca gccagacgct
     1321 gcaggcggtc gcgccctaac gaccggagaa cttggccttg cccggtccgt gctcgcggaa
     1381 cgactgcaca ccggtgcgcg cgtcctcggt accgaacact tcgacgaacg cttcggtctc
     1441 cgcgtcgagc gcggcggcga gcggcacacc acggccaccg ttgatgacgc gcttggcgag
     1501 ccccatcgca acgacggcgc ccttcgcgaa ctctgcggcc cagtgcagtg cgctgtgctc
     1561 gacttcgtcg tcgtgcacga ccttgtcgac gagtccgatg gcatgcgctt cgtcggcgcg
     1621 cacttgacgc cccgaccaga tcatctcctt ggcgcgcgcc gggcctacga gcgcgggcag
     1681 ccgctgggtt ccgcccgcgc cggggatgac gccgagcagg atctcgggct gtccgacacg
     1741 cgccttctcc gacgcgaccc gcaggtcgca cgccatcgcg agctcgagcc caccaccgag
     1801 cgcgtagccg cgcatcgctg cgatcaccgg gcggggaatg ctctcgaccg cgtcgaacgc
     1861 gtcgcgaaag ccgcgctcga tcacttgcgc ctgggcctgg tcgccgaact cgtcgatgtc
     1921 ggcgcccgcc gcgaatgcct tctcgctccc cgcgacgacg accgccttga cgctcgtgtc
     1981 gctcgcgaga tcgcgtgcga cgtcgcgcag cgcggccagc atcgcttgcg acagcgggtt
     2041 catcggcggc cggttcaagc gcaccagcgc gacaccgtgc tcgtcgcggt cacacaagac
     2101 atacgcgtcg tcggtcatgg gtgcggactc taggcgcggc gtggaaacag cacttcggct
     2161 gcgaccggcg tgaagcctcc cgcgagcgtg gcgcgcaacg atgccgcgtt gccgggcgcg
     2221 acctgcgccc acaccggttc gccgttcggg accagcgccc gagccgctgt gaccaggcga
     2281 cggccgagcc cttcgccccg cgcgctcgcg tcgacctcgt aaccgatctc ccaccgtccg
     2341 cagatgccgc gtccgagaat gatgacgccg gcaccgtcgg cggtcgcgaa gatctgcatg
     2401 tcgcggcggt atcgcgacgc gcgtgcgacg cgaggatggt cgaggtcgtc gacgcgcgtc
     2461 agccagtcgg gcatgccgtc gcccgtgccg agcgcgcaca acaaggcgtc gaacgttccg
     2521 ggccgagatc cgagctgcgc ggcgacccag ttcaggaaca ccgcagacat cggcgccgag
     2581 aagtcgccgt cgggcaaccg cgcgtgcacg agctcgggat cgatgtcggc cgccagcacc
     2641 cagtgcccgg tgaacccgac catcgcgtcg gcccggccgt cgaccgccgg cgcgacgtcg
     2701 atgacaccgt cgttcggagg gaaccgcccg tgggccgcgt cggcgagcac gcgcaccaaa
     2761 gggtgcccgt cagccatcgg cggtgagctc gtcg
//
LOCUS       DASUGA010000465         3095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_25923, whole genome shotgun sequence.
ACCESSION   DASUGA010000465 DASUGA010000000
VERSION     DASUGA010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25923"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..944)
                     /locus_tag="VFX21_09180"
     CDS             complement(120..944)
                     /locus_tag="VFX21_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020015451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5096174.1"
                     /translation="MTTVTTPVNLPRADESVRAAPAGYLHDLTSIAGRALRGVPRDFE
                     AVIPPVFIALFFFVVNIATLERLTSDVPGLDYKAFQMATAILLGVTGVSRAGALVLDV
                     QDNYFDRLLLTPVRRSAILLGHMAADVTVAACLTIPIVALGIILGVDFEGGPAGIVLF
                     IALAALWSLAFAGFGYAIALKTGNPAAVNSSFLLFFPFLFLTSSYVPRDQLSGWLDTV
                     AGCNPVTYILDGLRSLVLVPGWQWGDLAQALLAIGLVAALSFAMCFAALRGRVKRG"
     gene            complement(941..1912)
                     /locus_tag="VFX21_09185"
     CDS             complement(941..1912)
                     /locus_tag="VFX21_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5096175.1"
                     /translation="MGRPEDAAVRAIGLQRHFDGTEAVESLDLHVDQGEIYGFLGPNG
                     AGKSTTVRMLCTLLAPSGGQAFVAGHDVAAEPGAVRLRIGAALQDVALDPKQTGSELL
                     RLQGRLYGLKSRTITQRVAQLGELIDLGDALDRRIATYSGGMKRRLDLAAALVHNPAV
                     LFLDEPTTGLDPISRARVWTEIARLNRELGMTIFLTTQYLEEADALADRVGIIDHGRL
                     VAEGTPDELKRSVGTDLIVARVDGEAAPVVSSLRAIPGVIGVDAHGSELSIATDNGSA
                     TISPVAIALAKCGTGVRDITLRTPTLDDVFLDLTGSRIEHDTVEEAS"
     gene            complement(1966..2340)
                     /locus_tag="VFX21_09190"
     CDS             complement(1966..2340)
                     /locus_tag="VFX21_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HEX5096176.1"
                     /translation="MEMKLELVAVPVSDVDRAKAFYTEQVGFVADHDHRVSDDMRFVQ
                     CTPPGSACSIAFGQGITKAAPGSLEGLQVVVADVAQTRAELVERGVDVSEVEVFPWGS
                     FVHFADPDGNQWAVQQLPERDR"
     gene            2433..>3095
                     /locus_tag="VFX21_09195"
     CDS             2433..>3095
                     /locus_tag="VFX21_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX5096177.1"
                     /translation="MRADRLVATLLFLQSRGQVTAAEVAAELEVSVKTARRDLEALSI
                     AGIPVYSQPGRGGGWSLLGGARTDLSGLTAAEARTLFLVAGPSSAITPEAKAALRKLV
                     RALPETFRAEAEKAASAVVLDPAGWGRTAPSTPPHLEVLQQAVIAGVQVRLGYSDAKG
                     AVTERIVHPLGLVAKGTTWYLVGDTDNGRRTFRVWRVQSVELLDEPAVRPPDFDLHEA
                     WQE"
BASE COUNT          497 a         1034 c         1094 g          470 t
ORIGIN      
        1 gtcggcggcg cccgcttgat ccaggtcgta ctcgaagccg cactcggggc agcgctcgtc
       61 gtccatcgca ctgtcctact acggcggcgc gttcaccggc gggtgagggg ccgccgacgt
      121 cacccgcgct tgaccctgcc gcgcaacgcg gcgaagcaca tcgcgaagct cagcgccgcg
      181 accaaaccga tcgcgagcaa ggcttgcgcg aggtcgcccc attgccagcc gggcacgagc
      241 acgagcgatc gcagaccgtc gaggatgtac gtgaccggat tgcaccccgc gacggtgtcg
      301 agccatcccg agagctggtc gcgcggcacg tacgacgacg tgaggaacag gaacgggaag
      361 aacagcaaga agctcgagtt caccgcggcc gggttgccgg tcttcagtgc gatggcgtag
      421 ccgaagcccg cgaacgcgag gctccacagc gcggcgagcg cgatgaacaa gacgatgccg
      481 gccggtccgc cctcgaagtc gacgccgagg atgatgccga gcgcgacgat cggaatcgtc
      541 aagcacgcag ccaccgtgac gtcggccgcc atgtgcccca acaagatggc cgaccggcgc
      601 accggcgtga gcaagaggcg gtcgaagtag ttgtcctgca cgtcgagcac gagcgcgccc
      661 gcgcgcgaca cgccggtgac gccgagcagg attgccgtcg ccatctggaa cgccttgtag
      721 tcgaggcccg gtacgtcgga tgtcagccgc tcgagggttg cgatgttcac cacgaagaag
      781 aacaacgcaa tgaacacggg cgggatcacc gcttcgaagt cgcgcggcac gccccggagc
      841 gctcggcccg cgatcgacgt caagtcgtgc aggtagcccg cgggcgcggc gcgcaccgac
      901 tcgtcggcgc gcggaaggtt gacgggagtg gtgacggtcg tcatgaggct tcctccacgg
      961 tgtcgtgttc gatgcggctt ccggtgaggt cgaggaagac gtcgtcgagg gtcggcgtgc
     1021 gcagcgtgat gtcgcgtacg ccggtgccgc acttggccag ggcgatcgcg accgggctga
     1081 tcgtcgccga gccgttgtcg gtcgcgatcg acagctcgct gccgtgcgcg tcgacgccga
     1141 tgacgcccgg aatcgcgcgc aatgacgaga cgacgggcgc cgcttcgccg tcgacgcgcg
     1201 ccacgatcag gtcggtgccg accgatcgct tgagctcgtc gggcgtgccc tcggcgacga
     1261 ggcgtccgtg gtcgatgatg ccgacgcggt cggccagcgc gtccgcttcc tcgagatact
     1321 gcgtcgtgag gaagatggtc atgcccagct cccgattgag tcgcgcgatc tcggtccaca
     1381 cgcgggcccg gctgatcggg tcgagaccgg tcgtgggctc gtcgaggaac aacaccgcgg
     1441 ggttgtgcac gagcgcggcc gcgagatcga gtcggcgctt catcccgccc gagtaggtcg
     1501 cgatacggcg gtcgagcgcg tcgccgaggt cgatgagctc accgagttgc gcgacccgct
     1561 gcgtgatcgt gcgcgacttc aggccgtaga gccgaccctg caaccgcagc aactcgctgc
     1621 ctgtttgctt cgggtcgagc gcgacgtctt gcagtgcggc accgatgcgc aggcgcacgg
     1681 cgcccggttc ggctgcgacg tcatggcccg cgacgaacgc ctgcccgccg ctcggcgcca
     1741 gcaacgtgca caacatgcgc accgtcgtcg acttgccggc gccgttgggc ccgaggaatc
     1801 cgtagatctc gccctggtcg acgtggaggt cgaggctctc gaccgcctcg gtgccgtcga
     1861 agtggcgctg cagcccgatg gcgcgcacag cagcgtcttc gggacgtccc atatcggtac
     1921 tcctgatgat gaatgtggaa tcacgacgat cgatcagcgc ggaactcatc ggtcgcgctc
     1981 cggcagttgt tggaccgccc actggttgcc gtcggggtcg gcgaagtgga cgaacgagcc
     2041 ccacgggaag acctcgacct cgctcacgtc gacgccgcgc tcgaccagtt cggcgcgggt
     2101 ctgcgcgacg tcggccacga cgacctgcag cccctcgagc gagccgggcg ccgccttggt
     2161 gatgccctgg ccgaatgcga tggagcaggc ggaccccggc ggggtgcact gcacgaaccg
     2221 catgtcgtcg ctgacgcggt ggtcgtggtc ggcgacgaag ccgacttgct ccgtatagaa
     2281 ggccttggcc cggtcgacgt cggacacggg cacggcaacg agttccagct tcatctccat
     2341 gggggctccc ttcgggtggg gaagttcgat gtcttgcatc atccggggaa aagtggtcac
     2401 cccgtgtcca ggttccgtgg caatctggat gcatgcgagc cgaccgcctg gtggcgaccc
     2461 tgttgttcct gcagtcccgg gggcaggtga ccgcggccga ggtcgcggcc gagctcgagg
     2521 tctcggtcaa gaccgcgcga cgagacctcg aagccctgtc gatcgccggc atacccgtgt
     2581 actcacaacc gggccgtggc ggcgggtggt cgctgttggg tggtgcacgc accgacctct
     2641 ccggtctcac cgccgccgag gcgcgcacgt tgtttctcgt cgcgggcccg tcgtcggcga
     2701 tcacacccga agccaaggcc gcgctgcgca aactcgtgcg cgcgctgccc gaaacgttcc
     2761 gcgccgaagc cgagaaggcc gcgtcggccg tcgtgctcga tcccgcgggc tggggccgca
     2821 cggcgccgtc gacaccgccg catctcgaag tgctgcaaca agcggtgatc gcgggcgtgc
     2881 aggtgcggct cggttactcc gacgcgaagg gcgcggtgac cgaacgcatc gtacatccgt
     2941 tgggcctcgt cgcgaagggc accacgtggt atctcgtcgg cgacaccgac aacgggcggc
     3001 gcacgttccg cgtgtggcgg gtgcagtcgg tcgaactgct cgacgaaccc gcggtgcgcc
     3061 cgcccgactt cgacctgcac gaagcctggc aggag
//
LOCUS       DASUGA010000466         2551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1980303, whole genome shotgun sequence.
ACCESSION   DASUGA010000466 DASUGA010000000
VERSION     DASUGA010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1980303"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1507)
                     /gene="secF"
                     /locus_tag="VFX21_09200"
     CDS             complement(14..1507)
                     /gene="secF"
                     /locus_tag="VFX21_09200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX5096178.1"
                     /translation="MTTTMTPPPDDIPGVTRRHTFADLYHEHTNYQFIKHSKRWFILS
                     SIFMVIAIGGLVFRGLNLSIEFEGGTQWRVDMAEGRDATVGEVRDLLGPLGFDQAKVT
                     TLQGQGNTETVRVQAEVVRDPIRTIQRSLADVGQVADTDVTFTRAETGDGGAFTFTVD
                     EGHTITQADVEKALASTAAEDATVAVDDRTVTVTLDTIPSSPLTDVSEALAKYAGVDV
                     AQVTIDTVGPTWGETVTRNAVKAMIIFFLLLAVYLTFRFEWKMSISAIAAVVHDILFV
                     AGVYAVTQIEVSPATVTAFLTILGFSLYDTVVVFDKVRENQRALTATGRMTYGEMVNK
                     SLNSVLMRSLATSLVALLPVMSLLIVGSRIMGATALEDFAAALAAGLFIGAYSSIFVA
                     APLLAWWKEREPQYRALAERRRRITAAAPVPAMAGAPISDAAVEDALVEDAVIDDTVT
                     ADAGSVDAAAMGYTPRGKASEGIPGPPAVGRAPIAPRPRQQRRRKRH"
     gene            complement(1504..>2551)
                     /gene="secD"
                     /locus_tag="VFX21_09205"
     CDS             complement(1504..>2551)
                     /gene="secD"
                     /locus_tag="VFX21_09205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX5096179.1"
                     /translation="TAVPTTSVEQICSQLVATRAENVESASVWLPVQDENKNGRPDEG
                     CLYLGPSLMTGRDIKTASKRYSPDQGGPWVVDIEFKNDDFANLVAAPNVGRQVGIELD
                     GVVQSFPTINEGITGRNVQISGGFSEGEAKDLALVLKYGSLPVEFDADEQTVSSVSPS
                     LGKDQLHAGIVSGIVGLSLVAIYMIIYYRLLGVVVWVGLALTGLIFFTLVSYLSAHRG
                     LTLTLAGVTGIIVSVGITVDSYVVYFERLKDEVRSGKTVRSSLDSGFKKSFRTILAAD
                     LVSLLGAVVLYLLATGGVRGFAFFLGLSTVIDLLIAYFFMHPAVFLLARKPSLVRLPR
                     VGIAAALDVKEAVA"
BASE COUNT          439 a          837 c          868 g          407 t
ORIGIN      
        1 gcagcggggt tcgctagtgg cgcttgcgcc gtcgttgctg ccggggccgc ggcgcgattg
       61 gtgcgcggcc gacggcgggc ggcccgggga tgccttcgga ggccttgccg cgcggcgtgt
      121 agcccatggc ggcggcatcc acgcttcccg cgtccgcggt cacggtgtcg tcgatcacgg
      181 cgtcctcgac caaggcgtcc tcgaccgccg cgtcgctgat cggcgcgccg gccatggcgg
      241 gcaccggcgc ggcggccgtg atccggcggc ggcgctcggc cagcgcccgg tactgcggct
      301 cccgttcctt ccaccacgcg agcagcggcg cggcgacgaa gatcgaggag tacgcaccga
      361 tgaacagacc ggccgcgagg gccgcggcga agtcttcgag cgcggtggcg cccatgatgc
      421 gcgaaccgac gatgagcagc gacatcaccg gcaacagcgc gacgaggctc gtcgccagcg
      481 accgcatcaa caccgagttg agcgacttgt tgaccatctc gccgtacgtc atgcgtccgg
      541 tcgccgtgag cgcacgctgg ttctcgcgca ccttgtcgaa gacgacgacg gtgtcgtaca
      601 gcgagaaccc caagatggtg aggaacgcgg tgaccgttgc cgggctcacc tcgatctgcg
      661 tcacggcgta cacaccggcg acgaacaaga tgtcgtgcac caccgcggcg atggcggaga
      721 tcgacatctt ccactcgaag cggaaggtca ggtacaccgc gagcaacaag aagaagatga
      781 tcatcgcctt gacggcattg cgagtgacgg tctcacccca cgtcgggccg acagtgtcga
      841 tggtgacctg tgccacgtcg acgccggcgt acttggccag cgcctcgctg acatcggtga
      901 gcgggctgct cgggatcgtg tcgagcgtga ccgtgacggt gcgatcgtcg accgcaacgg
      961 tggcgtcctc ggccgcggtc gacgccagcg ccttctcgac gtccgcttgt gtgatcgtgt
     1021 ggccctcgtc gacggtgaac gtgaacgcgc cgccgtcgcc ggtctcggct ctcgtgaacg
     1081 tgacatcggt gtcggcgacc tgcccgacgt cggcgaggct cctctggatc gtgcggatgg
     1141 ggtcgcggac gacctcggcc tgcacgcgga cggtctccgt gttgccctgg ccctgcaacg
     1201 tcgtgacctt ggcttgatcg aagccgagcg gtcccaggag atcgcggacc tcgccgaccg
     1261 tggcgtcgcg gccctcggcc atgtcgacgc gccactgcgt accgccctcg aactcgatgc
     1321 tgaggttcag accccggaac acgagcccgc cgatcgcgat caccatgaag atgctcgaga
     1381 ggatgaacca ccgcttcgag tgcttgatga actggtagtt cgtgtgctcg tggtagaggt
     1441 cggcgaacgt gtggcgccgc gtcacacccg ggatgtcgtc gggcggcggg gtcatcgtcg
     1501 tcgtcacgcc acggcctcct tcacgtccag cgccgccgcg ataccgacgc ggggcaggcg
     1561 cacgagcgag ggtttccgcg ccaggaggaa caccgcggga tgcatgaaga aataggcgat
     1621 gaggaggtcg atcaccgtcg agaggccgag gaagaacgcg aagccgcgca ccccgcccgt
     1681 cgccaggagg tagaggacga cggcaccgag caacgacacc aggtcggcgg ccaggatcgt
     1741 gcggaacgac ttcttgaatc ccgagtcgag cgacgaccgc accgtcttgc cgctgcgcac
     1801 ttcgtccttc aatcgctcga aatagacgac gtacgagtcg acggtgatac ccaccgacac
     1861 gatgatgccc gtcacgccgg caagcgtgag ggtcaacccc cgatgcgccg aaagatacga
     1921 gacgagcgtg aagaagatca gaccggtgag cgcgagcccg acccatacga cgacgccgag
     1981 cagccggtag tagatgatca tgtagatcgc gacgagcgag agaccgacga tgccggacac
     2041 gatgccggcg tggagttggt ctttgccgag cgagggcgac acgctcgaga ccgtctgctc
     2101 gtcggcgtcg aactccacgg gcagcgaccc gtacttgagg acgagcgcga ggtccttggc
     2161 ttcaccttcg ctgaacccgc ccgagatctg cacgttgcgc ccggtgatgc cttcgttgat
     2221 cgtcgggaac gactgcacga caccgtcgag ctcgatgccc acctggcgac cgacgttcgg
     2281 tgccgcgacg agattggcga agtcgtcgtt cttgaactcg atgtcgacga cccacggacc
     2341 cccctggtcg gggctgtagc gcttcgacgc ggtcttgatg tcgcggccgg tcatcagcga
     2401 cgggcccagg tagagacagc cttcgtcggg cctgccgttc ttgttctcgt cctggaccgg
     2461 taaccagacc gacgcggact cgacgttctc cgcacgcgtc gcgacgagtt gcgagcagat
     2521 ctgttcaacc gacgtcgtcg gtaccgccgt c
//
LOCUS       DASUGA010000467         2257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_14217, whole genome shotgun sequence.
ACCESSION   DASUGA010000467 DASUGA010000000
VERSION     DASUGA010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14217"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2214)
                     /locus_tag="VFX21_09210"
     CDS             complement(<1..2214)
                     /locus_tag="VFX21_09210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEX5096180.1"
                     /translation="MYSEPPAVFAPAPKPKPKDRLRAIGSLVTAIVVVAAGVVVVATS
                     SAGDPIFPKKWDPRVAPVAAAVAKLRGLQFQHPVPVKFLEPKEFEKEVGVDQTSVTAE
                     DRAEIEEQAAGLRALGLIGGDVDLLKAVDTAQKSSTLALYSFRNKEILVRGSTLDAQR
                     RVTLAHELVHVLQDQHFDLTKLQRKAYESKVGDTSVFTALVEGDASRTEELYRDKMSA
                     KDKAEYDRADAAEGARVKKESKGVPEIVSFVFGAPYAFGPAHATVLAETGGNVAINRA
                     LVAPTPTSLIFIQPGNTAAGVAVPEPVIPAGTKPVGSPTAFGAFELYITLGMYLDANR
                     ALHVADGVAGGRQTQFKRGNKVCVKVDLAGERASAEAAARNALQSWANLQPKEAEAKV
                     STNALGFTACDPGKKASAPNGARFTSLAETLGLRTGLTQGAAQNDLDAPAARCAARLY
                     MTHPDAVAVLRQLGDGQPSPTQQALITGTLVQSARACVQDENAGLPLPSLFGTERRAN
                     IAPVTDDSDLAGLDPYALLEGEAVRVDEHFSALEGGAWDEPSRCEGWSVRDLLAHLAA
                     TETYTRACLDGTVQALFAELGEKGATDLPSFNEVGIREYDGQSPQEVLAEWRATSAAN
                     RAGFRERGNGMVDSSVGDYPARWQEFHLAFELATHADDAHIPASKAVQADRLAWQAQF
                     GRFAFKEHHPDAIIEARDGRVHVKTDAVEADLSEEEWVQVLSARVPAGSRLDPALA"
BASE COUNT          339 a          771 c          747 g          400 t
ORIGIN      
        1 cgcgagcgcg ggatcgagcc ggctcccggc cggaaccctg gcactgagta cttgcaccca
       61 ttcctcctcg ctgaggtcgg cttcgaccgc gtcggtcttc acgtgcacgc gcccgtcgcg
      121 ggcctcgata atcgcatcgg gatggtgttc cttgaaggcg aaccggccga attgcgcttg
      181 ccacgcgagc cggtccgcct gcacagcctt cgatgccggg atgtgcgcgt cgtcggcatg
      241 ggtggcgagt tcgaacgcga ggtgaaactc ctgccagcgc gccggatagt cgcccactga
      301 cgaatcgacc atgccattgc cccgctcgcg aaagccggct cggttcgccg cgctcgtggc
      361 gcgccactcg gccagcacct cctgcgggct ctgcccgtcg tactcgcgga tcccgacctc
      421 gttgaacgag gggagatcgg tcgcaccttt ctcgcccagt tccgcgaaca acgcttgcac
      481 cgtcccgtcg aggcacgcac gcgtgtacgt ctccgtcgcg gcgagatggg cgaggagatc
      541 gcgcacgctc cagccctcgc agcgggacgg ttcgtcccac gccccgccct ccaatgcgga
      601 gaagtgctcg tcgacacgca ccgcttcgcc ttcaagcagc gcgtagggat cgagcccggc
      661 cagatccgaa tcatccgtca cgggcgcgat gttcgcgcgc cgttcggtgc cgaacaacga
      721 aggcaacggc aggccggcgt tttcgtcttg cacgcaggcc cgtgcgctct gcacaagcgt
      781 gccggtgatc agcgcctgct gcgtcggaga cggctgaccg tcgccgagct ggcgcagcac
      841 tgccaccgcg tccgggtgcg tcatgtacag gcgcgcggca caccgggccg ccggcgcgtc
      901 gaggtcgttc tgcgccgcac cttgggtgag cccggtgcgc aaacccaacg tctcggccaa
      961 ggatgtgaag cgggcaccgt tgggcgcact cgccttcttg ccggggtcgc acgccgtgaa
     1021 accgagcgcg ttggtcgaga cctttgcctc ggcttctttg ggctgcaggt tcgcccatga
     1081 ctggagcgcg ttgcgagccg ccgcttctgc actcgcgcgc tcgccggcga ggtcgacctt
     1141 gacgcagacc ttgttgccgc gcttgaactg cgtttgccgc ccgcccgcga cgccgtcggc
     1201 aacgtgaagc gcccgattgg cgtcgaggta catcccgagc gtgatgtaaa gctcgaacgc
     1261 gccgaacgcc gtgggacttc cgaccggctt cgtcccggcc gggatcacgg gctcggggac
     1321 cgcaacgccg gccgccgtat tcccgggctg gatgaagatc aggctcgtcg gagtcggcgc
     1381 aaccagcgcg cggttgatcg cgacgttccc gccggtttcg gcgagcaccg tggcgtgcgc
     1441 cggtccgaac gcgtacggag cgccgaacac gaacgacacg atctccggaa cgcctttgct
     1501 ttctttcttg acccgcgcac cctcggcggc atcggcccgg tcgtattccg ctttgtcctt
     1561 ggccgacatc ttgtcgcgat agagctcttc ggtgcgcgac gcatcgcctt cgacgagcgc
     1621 ggtgaagacc gacgtgtcgc cgaccttcga ctcgtacgcc ttgcgttgca acttcgtgag
     1681 atcgaagtgt tggtcctgca ggacgtggac gagctcgtgc gcgagcgtga cgcggcgctg
     1741 cgcatcgagc gtcgagcccc gcaccaggat ctccttgttg cggaagctgt agagcgcgag
     1801 cgtggacgac ttctgggcgg tgtcgacggc tttgaggaga tcgacgtcgc caccgatcag
     1861 accgagcgct cgcaatccgg ccgcctgttc ctcgatctcg gcgcggtctt cggccgtgac
     1921 ggaagtttgg tcgacaccga cttccttctc gaactccttc ggttccaaga acttcaccgg
     1981 caccggatgc tggaactgga gtccgcgcag tttcgcgact gccgcggcaa cgggcgcgac
     2041 gcgcgggtcc cacttcttcg gaaagatcgg atcgccggcc gaggacgtcg cgactacaac
     2101 gacgcccgcc gcgacgacca cgattgcggt gacgagcgag ccgatcgctc gcagacggtc
     2161 tttgggcttg ggcttgggcg cgggagcgaa gacggccggc ggttcggagt acatcaagtt
     2221 cccatcggcc ggttgttgcc ccgcatcagg aggactg
//
LOCUS       DASUGA010000468         2161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_326817, whole genome shotgun sequence.
ACCESSION   DASUGA010000468 DASUGA010000000
VERSION     DASUGA010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326817"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1904)
                     /locus_tag="VFX21_09215"
     CDS             complement(<1..1904)
                     /locus_tag="VFX21_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096181.1"
                     /translation="MHARSLVLALSSLGALAPSQSPSAPRTTAGALTPLSTTAPPRTL
                     HVDTTAGGTVWARGADWKAAFSPAGLEFVPFLGSDAERNFPLRLQLASADAGGVALPL
                     ELQHTARVAGERVEIARGALVEAYDLALDHVEQSFVFERPVPGGALRVRMRVDSELAG
                     ANDESGGFRFANARGGVRYGEAVAFDAAGRRTAVASTCEGGSLVLTVPADFVASATFP
                     LTIDPVVSTFSATPAPISLDQSNGDCAYLGTYFGLYAAVVEEHYSQTDHDILVTARDP
                     DNLIVTSVYVDYTTDYWATPSIASHRGASQFLVAAAKGLPTVNSRTIQGRTLTWNGGG
                     VLTMSNQFPIQSFGNGMNPHVGGDANPSPTPPTGYCVTWTGTIDGELYYNLVRTDTSL
                     MATNGGWLDPGPEFVSNPSISKSCGIGAFASQEWLIVWQKRYSPTDEDIHGTRIGVDG
                     AVRTLDFLIDYSGQSDTNPAVSSLTDTTGAGVERFAVVYQRDFPATTLTPQHSDIMGQ
                     LFATTTSVTGTVNLTQLLNGSIFDNHYKPCIDTDGQRFALGFTQWGSIFSADTEPYLA
                     TLHVVDDTFQVTAYPELCNAYHGPDEQMRITSERSGGTFSPRYLAVWHTEHTTPPSGA
                     GVLGSFYYGH"
     gene            complement(1977..>2161)
                     /locus_tag="VFX21_09220"
     CDS             complement(1977..>2161)
                     /locus_tag="VFX21_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096182.1"
                     /translation="PLGALAAASAANLLSAGKDRAALECLARAHERLGEAAAAKAWRE
                     RAAALPAAPQPTNGAR"
BASE COUNT          291 a          720 c          819 g          331 t
ORIGIN      
        1 atgtggccgt agtagaacga gccgagcacg ccggcgccgc tcggcggcgt cgtgtgctcg
       61 gtgtgccaca cggccaagta gcgcggcgag aacgtgccgc cgctgcgctc cgacgtgatc
      121 cgcatctgct cgtcgggccc gtggtacgcg ttgcacagct ccgggtacgc ggtgacctgg
      181 aacgtgtcgt cgaccacgtg cagcgtcgcg aggtacggct cggtgtcggc cgagaagatc
      241 gagccccact gcgtgaagcc gagcgcgaag cgctggccgt cggtgtcgat gcacggcttg
      301 tagtggttgt cgaagatgct gccgttcagc agttgcgtga ggttcacggt gccggtcacc
      361 gacgtcgtcg tcgcgaacag ctggcccatg atgtcgctgt gctgcggcgt cagcgtcgtc
      421 gccgggaagt cgcgctggta gacgacggcg aagcgctcga cgccggcgcc ggtcgtgtcg
      481 gtcagcgagc tgaccgcggg gttcgtgtcg ctctggcccg agtagtcgat caggaagtcg
      541 agcgtgcgca ccgcgccgtc gacgccgatg cgggtgccgt ggatgtcctc gtcggtcggg
      601 ctgtagcgct tctgccagac gatcagccac tcctggctcg cgaacgcgcc gatgccgcac
      661 gacttgctga tcgacgggtt gctgacgaac tcgggtccag ggtcgagcca gccaccgttc
      721 gtcgccatca gcgacgtgtc ggtgcgcacg aggttgtagt acagctcgcc gtcgatcgtg
      781 ccggtccacg tcacgcagta gccggtcggc ggcgtcgggc tcgggttcgc gtcgccgccg
      841 acgtgcgggt tcatgccgtt gccgaacgac tggatcggga actggttgct catcgtcagc
      901 acgccgccgc cgttccaggt cagcgtgcgg ccctggatgg tccgcgagtt caccgtcggc
      961 aggcccttcg ccgccgcgac gaggaactgg ctcgcgccgc gatggctcgc gatcgacggc
     1021 gtcgcccagt agtcggtcgt gtagtcgacg tagacggacg tcacgatcag gttgtccggg
     1081 tcgcgcgcgg tgacgaggat gtcgtggtcg gtctggctgt agtgctcctc gaccaccgcc
     1141 gcgtagaggc cgaagtacgt gccgaggtac gcgcagtcgc cgttgctctg gtcgagcgag
     1201 atcggggcgg gcgtcgcgct gaacgtcgac acgacggggt cgatcgtcag cgggaacgtc
     1261 gccgatgcga cgaagtccgc ggggaccgtc agcacgagcg agccgccttc gcacgtgctc
     1321 gcaaccgcgg tgcgccggcc ggccgcgtcg aatgccaccg cctcgccgta gcgcacgccg
     1381 ccgcgcgcgt tcgcgaagcg gaagccgccg ctctcgtcgt tcgcgccggc gagctcgctg
     1441 tcgacgcgca tgcggacgcg cagcgcaccg ccgggcacgg gacgctcgaa cacgaacgac
     1501 tgctcgacgt ggtcgagcgc gaggtcgtac gcctcgacga gcgcgccgcg cgcgatctcg
     1561 acgcgttccc cggcgacccg cgcagtgtgc tgcagctcga gcggcagcgc gacgccgccg
     1621 gcatcggcac tggcgagctg cagtcgcaac gggaagttgc gttctgcatc cgagccgagg
     1681 aacggcacga actcgagacc ggccggcgag aacgcggcct tccagtccgc gccgcgggcc
     1741 cacacggtgc cgccggcggt cgtgtcgacg tgcagcgtgc gcggcggcgc ggtggtggac
     1801 agcggcgtca gcgcgcccgc ggtggtgcgg ggcgcggacg gggactggct cggcgcgagt
     1861 gctccgagcg acgacagggc gagtacgagg gatcgggcgt gcatttcgtg gactccttgc
     1921 ccgcggcagc ggggcgccgg ggaaggagcc gcagcggcgg cgaccgaacg actttctcag
     1981 cgtgcgccgt tcgtcggctg gggagcggcg ggcaacgcgg ccgcgcgttc gcgccacgcc
     2041 ttcgccgccg ccgcctcgcc gaggcgttcg tgcgcgcgcg cgaggcactc gagcgctgcg
     2101 cgatccttgc cggcgctgag caggttcgcc gcgctcgcgg cggcgagtgc accgagcgga
     2161 t
//
LOCUS       DASUGA010000469         2413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1560568, whole genome shotgun sequence.
ACCESSION   DASUGA010000469 DASUGA010000000
VERSION     DASUGA010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560568"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFX21_09225"
     CDS             complement(<1..234)
                     /locus_tag="VFX21_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5096183.1"
                     /translation="MTNNVVHFAIHADDLERAQRFYRGVFGWRFEAWGPPGFYRIHTG
                     TADDPGIEGALHDRAEPLTGTGMRGFLCTVSVDD"
     gene            275..1030
                     /locus_tag="VFX21_09230"
     CDS             275..1030
                     /locus_tag="VFX21_09230"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5096184.1"
                     /translation="MATRNVENQPAENDLIRPLKVAYPAGHRLPVHTHEWGQLAYAAT
                     GFLTVDAAAATWVVPADRAAWLPPGTPHSLSTRVRTVLHSIYFPPGARGLGKETRVVE
                     VTPLVREIIDHVVAREQLDLRTAADRRLVQVLVDQLRTIDVAPLELRQPFDAVARDAA
                     DRLRADPARDPDDVARAVGTSRRTLERRFREETGTPLGRWRQRAQVLRAVELLAGGAP
                     VTEAATTVGYSTPSAFVVAFRTVFGTTPGRFFA"
     gene            complement(1037..1783)
                     /locus_tag="VFX21_09235"
     CDS             complement(1037..1783)
                     /locus_tag="VFX21_09235"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5096185.1"
                     /translation="MCFTEGMDEGFSLEKLVERSGVGVRTIRYYQSEGLLPKPTKQGR
                     DAVYTDEHVDRLALIAELRDRGLTLSAIRELVASDRPRRTANEWLGIDATLKAPWSDD
                     RPRVMEREELTKLIGRRAGLLGELEDAGYVKPGDADTWTVPSPGLLDLALQLHDAGID
                     VDLSGQLRDLLRRRISKAVDDAVALLAERTGAGFAGRATPDELAHAIGALRPVARETT
                     SLILAQEVERALRQFLDQGPEKVKAKVRRK"
     gene            1856..>2413
                     /locus_tag="VFX21_09240"
     CDS             1856..>2413
                     /locus_tag="VFX21_09240"
                     /inference="COORDINATES: protein motif:HMM:NF023015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HEX5096186.1"
                     /translation="MTDTVTFPRTAHRATQLVAASERTTLDPFTAVDWSVPIDDAAYH
                     LPPEMLPLFGTDAWHAMSDTERIAYSRHETAALCGAGIWFENVLMQAVLRHLAEVPVT
                     DPAHRYLLVEVADECRHSMMFGEYIRRAGTPAYTPGIDPIHVRDLPGGRAMSYLLILA
                     VEELLDYINRATMRDERVHPVSRQIA"
BASE COUNT          368 a          875 c          771 g          399 t
ORIGIN      
        1 gtcgtcgacc gagaccgtgc acaggaagcc gcgcatcccg gtgccggtca acggttccgc
       61 gcgatcgtgc aacgcccctt cgatgccggg atcgtcggcg gtgccggtgt ggatccggta
      121 gaagcccggc ggtccccatg cttcgaagcg ccagccgaag acgccgcggt agaagcgttg
      181 ggcccgctcg aggtcgtcgg cgtggatggc gaagtggacg acgttgttgg tcatggccct
      241 accgtacgga cccgcagtga ggcacacttt cgaaatggcg acaagaaatg tcgaaaatca
      301 gccagccgag aacgacctga tccggcccct gaaagtcgcc tacccggccg gtcaccgcct
      361 gccggtgcac acccacgagt ggggccagct cgcgtacgcg gccacgggtt tcctcaccgt
      421 cgacgcggcc gctgcgacct gggtcgtgcc cgccgatcgc gccgcctggt tgccgccggg
      481 tacaccgcat tcgctgtcga cgcgcgtgcg caccgtgctg cactcgatct acttcccgcc
      541 cggcgcgcgc ggactcggca aggagacgcg cgtcgtcgaa gtgacgccgc tcgtgcgtga
      601 gatcatcgac cacgtcgtcg cacgcgaaca gctcgacctg cgcactgctg ccgaccgtcg
      661 gttggtgcag gtgctcgtcg atcagctccg cacgatcgac gtcgcaccac tcgaactgcg
      721 tcaacccttc gacgccgtcg cacgggatgc cgccgaccgg ctgcgcgccg atcccgcgcg
      781 cgatcccgac gacgtcgcgc gcgcggtcgg tacgagccgc cgcacactcg agcggcggtt
      841 ccgcgaagag acgggcacgc cgctcggccg ctggcgtcag cgcgcgcaag tgttgcgcgc
      901 ggtcgagttg cttgcgggcg gcgcgccggt caccgaggcc gcgacgaccg tcggctattc
      961 cacaccgagc gcgttcgtcg tcgcgttccg cactgtcttc ggcacgacgc cgggccggtt
     1021 cttcgcctga ctgcggctac ttccggcgga ccttggcctt gaccttttcc ggtccttggt
     1081 cgaggaactg acgcagcgct cgttcgacct cttgcgccag gatcaggctc gtcgtctcgc
     1141 gcgccacggg gcgcaacgcg ccgatcgcat gcgcgagctc gtcgggagtc gcgcgtcccg
     1201 cgaagccggc gccggtccgt tccgcgagga gtgcgaccgc gtcgtcgacc gctttcgaga
     1261 tacgccgccg caacaggtcc cgcagttgtc cgctcaggtc gacgtcgatt ccggcgtcgt
     1321 gcagctggag cgcgaggtcg agaagacccg gactcggcac ggtccaggtg tccgcgtcgc
     1381 ccggtttgac gtagccggca tcctcgagct cgccgagcag accggcgcgc cgcccgatca
     1441 actttgtcag ctcttcgcgt tccatcacgc gcggccggtc gtcggaccac ggtgccttca
     1501 acgtcgcgtc tataccgagc cactcgttcg cggtccgccg cgggcgatcg gacgccacga
     1561 gctcgcggat cgccgacaac gtcaacccgc ggtcacggag ctccgcgatc aacgcgaggc
     1621 gatcgacgtg ctcgtcggtg tagaccgcgt cgcggccctg cttcgtcggc ttcggcagga
     1681 gcccttcgga ctggtagtac cgaatcgtcc ggacgccgac gccgctgcgt tcgacgagtt
     1741 tttcgagcga gaagccctcg tccatgcctt cagtgaagca cacatggcca gatgattaca
     1801 gtacgtactg taagaatatc ttgacagtgc ctactgtaac tgtttgggtg cagacatgac
     1861 cgacaccgtc accttccccc ggacggcgca tcgggccacc cagctggtcg ccgcctccga
     1921 acgcacgact ctcgatccct tcaccgccgt cgactggtcg gtgccgatcg acgatgccgc
     1981 ctaccacctc ccgcccgaga tgctgccgct gttcggcacc gatgcctggc acgccatgtc
     2041 ggacaccgag cgcatcgcgt acagccgcca cgaaacggct gcgctgtgcg gcgccggcat
     2101 ctggttcgag aacgtgctga tgcaagccgt gctccgccac ctcgccgaag tgcccgtgac
     2161 cgatcccgcg caccgctacc tgctcgtcga agtcgcggac gagtgccgcc actcgatgat
     2221 gttcggcgag tacatccgcc gcgccggcac gcccgcgtac acgcccggca tcgacccgat
     2281 ccacgtccgc gacctgcccg gtgggcgcgc gatgtcgtac ctgctgatcc tcgcggtcga
     2341 ggagctcctc gactacatca accgcgccac gatgcgcgac gaacgagtgc acccggtctc
     2401 ccgtcagatc gcg
//
LOCUS       DASUGA010000470         3051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_265175, whole genome shotgun sequence.
ACCESSION   DASUGA010000470 DASUGA010000000
VERSION     DASUGA010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265175"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..164
                     /locus_tag="VFX21_09245"
     CDS             15..164
                     /locus_tag="VFX21_09245"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5096187.1"
                     /translation="MWWGAHDNPGAGFWQRQFLWSKAASIAGGTSEVQRTIIGDRMLG
                     LPRSP"
     gene            175..549
                     /locus_tag="VFX21_09250"
     CDS             175..549
                     /locus_tag="VFX21_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018559762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5096188.1"
                     /translation="MPIVKVVGLDHIVLRVADVEKSLAFYIDTLGLEPVRVEEWRRGE
                     VLFPSVRLTPETIIDVFAGERSGENLDHFCMVIEPTDLDALATRFPDAQRGDGLFGAQ
                     GYASSLYIHDPDGNTVELRSYS"
     gene            546..1178
                     /locus_tag="VFX21_09255"
     CDS             546..1178
                     /locus_tag="VFX21_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03085 family metal-binding protein"
                     /protein_id="HEX5096189.1"
                     /translation="MSATSALLAERAALCDTLAGLGPDAPTLCEGWQTLDLAAHLVAR
                     EARSDAALGLVLPGPFARHLQTVMDRYKERGYDTLVGMLRTGPPKMHRMGPLAAANVN
                     ENFVHHEDARRPAGLGPRELDRETEAILWKMLGFGRRLAKRRLKGAGLVLRRPDGQEC
                     VVSDAQPRVTVTGATGELVLFMSGRKEAAAVEHEGDPEAVAIVLATHFGI"
     gene            complement(1175..1993)
                     /locus_tag="VFX21_09260"
     CDS             complement(1175..1993)
                     /locus_tag="VFX21_09260"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5096190.1"
                     /translation="METDEVLETGYGPRTPRGDNLTNDFAQGEADAFAALADARGDRR
                     LDDDEFVLMLRDGASGSPFGNVAVLRRPLSDDEWRAAATRMHDFFAQGPGGPFMVFSA
                     WPTPNLRDLGFGLVGHPPMMLRPAGPRELPLPDGLEIRRVDDAASAADWEYVMVHGYP
                     VPEVQPFAPGCILPESALAAEGWRHFLGVVDGKPVATGSAYVDPQHIHVEFISALDEA
                     RGRGIGAALTTVATTTEIDRPAMLIASDAGKPTYDRMGYQTLSRFTLWAGHRRS"
     gene            2056..>3051
                     /locus_tag="VFX21_09265"
     CDS             2056..>3051
                     /locus_tag="VFX21_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5096191.1"
                     /translation="MKELVYHRQLLPAVERYGAKVAFVDGDYRATYEEHFDRVSRLVG
                     ALQGLGIAPEDRFAVMALNSHQFLELYHAAFLGAGVVNPLNLRLAPKELAYILADSGT
                     KVCFTDPFFAPVIAQVREEAGLEHVVMIGDGDAPHDHSYEELLGAAKAIVPDEPEEDE
                     PVILMYTGGTTGLPKGVLLEHRAEMLNLYHVAFEFPVLEDEVFLHQTPMFHAASMGGI
                     LGVPSVGGVSVFQSLFNPAEVLDRIEEHSVTMTVMVPTMIGMMVNHPDYRPERLMSLR
                     TLTYGASPMPTALLEKLLRELPDISIYQGYGMTECSAVLTVLNADDHRHGGDLLRS"
BASE COUNT          474 a         1029 c         1065 g          483 t
ORIGIN      
        1 gtgccgccgc gccgctgtgg tggggtgcgc acgacaaccc cggcgcgggg ttctggcaac
       61 ggcagttctt gtggtcgaag gcggcgtcga tcgcgggcgg cacgtccgag gtgcagcgca
      121 ccatcatcgg cgaccgcatg ctcggcctcc cgcgttcgcc ctagggtgca aggcatgcca
      181 attgtcaagg tcgtcggtct cgatcacatc gtgttgcggg tcgccgacgt cgagaagtcg
      241 ctcgcgttct acatcgacac cctcgggctc gaaccggtgc gcgtcgagga gtggcggcgg
      301 ggtgaagtgc tgttcccgtc ggtgcggttg acgccggaga cgatcatcga cgtcttcgcc
      361 ggcgagcgca gcggtgagaa cctcgaccac ttctgtatgg tcatcgaacc gaccgatctc
      421 gacgcgctcg ccacgcgatt tccggacgcg cagcgcggcg acggcctgtt cggcgcccag
      481 ggttacgcgt cgagtctcta catccacgac cccgacggca acacggtcga actgcggtcg
      541 tactcgtgag cgcgacctcg gcgctcctcg cggaacgggc cgcgttgtgc gacacgctcg
      601 ccgggctcgg ccccgacgcg ccgacgctgt gcgaggggtg gcagacgctc gacctcgccg
      661 cgcacctcgt cgcgcgtgaa gcgcggtccg acgccgcgct gggtctggtg ctgcccggtc
      721 cgttcgcgcg acacctccag accgtgatgg accgctacaa ggaacgcggc tacgacacac
      781 tcgtcggcat gctgcggacg ggcccgccga agatgcaccg catgggaccc ttggccgccg
      841 cgaacgtcaa cgagaacttc gtccaccacg aagacgcgcg ccgccccgcg ggtctcggcc
      901 cgcgtgagct cgaccgcgag accgaggcga tcctgtggaa gatgctcggc ttcggccggc
      961 gtctcgcgaa acgacggctc aagggtgcgg gcttggtact gcgcagaccg gacggtcagg
     1021 agtgcgtggt ctccgacgcg caaccgcgcg tgacggtcac gggagcaacg ggcgagctcg
     1081 tcctgttcat gagcggccgc aaggaagccg cagccgtcga acacgaagga gatccggaag
     1141 cagtcgcgat cgtcctcgcg acgcacttcg gtatctagct tcggcggtgg cccgcccaga
     1201 gggtgaagcg gctcagcgtt tggtagccca tgcggtcgta ggtcggtttg cccgcgtcgc
     1261 tcgcgatcaa catggccggg cggtcgatct ccgtcgtcgt cgccaccgtg gtgagcgcgg
     1321 cgccgatgcc gcgaccgcgc gcttcgtcga gcgccgagat gaactcgaca tggatgtgct
     1381 gcgggtcgac gtaggcggaa cccgtcgcga cgggcttgcc gtcgaccaca ccgaggaagt
     1441 ggcgccaacc ttccgccgcc agcgcggatt ccgggaggat gcaacctggt gcgaacggct
     1501 gcacctcggg gacggggtaa ccgtgcacca tgacgtactc ccagtcggcg gcgctcgcgg
     1561 cgtcgtcgac gcggcggatc tccagcccgt cggggagtgg cagctcgcgc gggcccgcgg
     1621 gccgcagcat catcggcgga tggccgacga gcccgaagcc gaggtcgcgc agattcgggg
     1681 tcggccacgc ggagaacacc atgaacggcc cgcccggtcc ctgggcgaag aagtcgtgca
     1741 tgcgcgtcgc cgcggcccgc cattcgtcgt cggagagtgg ccggcgcaag accgcgacgt
     1801 tgccgaacgg agagcccgac gcaccgtcgc gcagcatcag gacgaactcg tcgtcgtcga
     1861 gccggcgatc accgcgagcg tcggcgagcg cggcgaaggc gtcggcttcc ccctgggcga
     1921 agtcgttcgt gaggttgtcg ccgcggggcg tgcgcgggcc gtagccggtc tcgaggactt
     1981 cgtcggtctc cacgctgcca gtcttacggg cggccgggct agcgtcgagg cgtcgcgagg
     2041 accacggggg gaaccgtgaa ggagctcgtc taccaccgcc agctgctccc ggccgtcgag
     2101 cgctacggcg ccaaggtcgc gttcgtcgac ggcgactacc gcgccacgta cgaggagcac
     2161 ttcgaccgcg tctctcgcct cgtcggtgcg ttgcaagggc tcggcatcgc acctgaggac
     2221 cgcttcgcgg tgatggccct caacagccat cagttcctcg agctctatca cgctgcgttc
     2281 ctcggcgcgg gcgtcgtcaa cccgctgaac ctgcggctgg cgccgaaaga gctggcgtac
     2341 atcctcgccg actccggcac gaaggtctgc ttcaccgacc cgttcttcgc gcccgtgatc
     2401 gcgcaggtgc gcgaggaagc cgggctcgaa cacgtcgtga tgatcggcga cggtgacgcg
     2461 ccgcacgacc actcgtacga ggagttgctc ggcgccgcga aggcaatcgt gcccgacgag
     2521 cccgaagagg acgagccggt catcctcatg tacacgggcg gcacgacggg cttgccgaag
     2581 ggcgtgttgc tcgaacaccg cgccgagatg ctgaacctgt atcacgtcgc gttcgagttt
     2641 ccggtgctcg aagacgaagt gttcctccac cagacgccga tgttccacgc ggcgtcgatg
     2701 ggcggcatcc tcggcgtgcc gtcggttggt ggcgtgtcgg tgttccagtc gctcttcaac
     2761 ccggccgagg tgctcgaccg catcgaggag cactcggtca cgatgacagt gatggtgccg
     2821 acgatgatcg gcatgatggt caaccacccc gactaccggc ccgagcggct gatgtcgctg
     2881 cgtacgctca cctacggcgc gtcacccatg ccgaccgcgc tgttggagaa gctgttgcgc
     2941 gagctgcccg acatctcgat ctatcagggc tacggcatga ccgagtgttc cgcggtgctc
     3001 accgtgctga acgccgacga ccaccggcac ggtggcgacc tgttgcgatc g
//
LOCUS       DASUGA010000471         2486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1547339, whole genome shotgun sequence.
ACCESSION   DASUGA010000471 DASUGA010000000
VERSION     DASUGA010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547339"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1923)
                     /gene="nuoG"
                     /locus_tag="VFX21_09270"
     CDS             complement(<1..1923)
                     /gene="nuoG"
                     /locus_tag="VFX21_09270"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEX5096192.1"
                     /translation="MTATQPQEQEQPKKDTVTVTVDGVTFEAKPGELLIKAAQEHGIY
                     IPRFCWHERMKPVGMCRMCLVEVDGMRGYPPACTTPVAEGMVAHSQSDVVRKIQDGVL
                     EFLLINHPLDCPVCDRGGECPLQDQTLAFGPGESRFVEEKRHFEKPIPLSKLVLLDRE
                     RCIQCGRCTRFADEIAGDPLIDFLDRGEHMQVLNFAGQPFDSYFSGNTVQICPVGALT
                     ASQYRFRARPWDLSTAETSCTTCAVQCRGALQSSSNRLVRLLGVDSEPVNQGWLCDKG
                     RYNIEWVHSDARRIEPAVKVQNQLVETSWPEALDVVVTELKKSIELHGPGSVAVIGGA
                     RGTNEDAYAWSRLAKCVVGTDNVDAQLGDGLPADAVLGLPRAEISDCDRAAAIVILDA
                     GLEDSVPVLALRLRRAVKELKVPLVDCAPVAHKLSEHAALVARTLPGEPIADSAISGI
                     GRLVAGNDGPVVVVLGRGSVAESVHATLRTAALLSGLPNVRFLSALRRGNVHGALDAG
                     LAPGFLPGRVSLSGGGQWFADRWGNVPADTGLDAEGILRAAAEGKIHVLVLLGANPLT
                     DFPDAALAKRGIDGASIVVAVDAFVPDGARTPDVLLPCTVWGEKSGTVTNLEGRVQRV
                     SRKVAPEGTAMDDWRIA"
     gene            complement(1920..>2486)
                     /locus_tag="VFX21_09275"
     CDS             complement(1920..>2486)
                     /locus_tag="VFX21_09275"
                     /inference="COORDINATES: protein motif:HMM:NF022059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX5096193.1"
                     /translation="HVKNPGVFEVEMGCTFRDIIYGLGQGIRDDKELKFFLPGGASFQ
                     WLLGIDEHLDAPYDIDWVQQNLGTTLGSGAIMVFDETTDPVAVAWRLAKFYAHESCGK
                     CTPCREGTGWIEKVLFRIANGLGRPGDLEMLLDVGAGISPNVATAPFTQTTLCPLGPS
                     VVSPLASLDKYFRDEIIARINSDAGVTV"
BASE COUNT          380 a          847 c          887 g          372 t
ORIGIN      
        1 cgcgatgcgc cagtcgtcca tcgcggtgcc ttcgggcgcg accttgcggc tcacgcgttg
       61 cacgcgacct tcgagattcg tgacggtgcc cgacttctcg ccccacaccg tgcacggcag
      121 gagcacgtcg ggggtgcgcg cgccgtcggg cacgaacgcg tcgaccgcca cgacgatcga
      181 cgcgccgtcg atgccgcgct tggccagtgc ggcgtcgggg aagtcggtga gcgggttggc
      241 gccgagcagc acgagcacat ggatcttgcc ctcggcggcg gcgcgcagga tgccttcggc
      301 gtcgaggccg gtgtcggcgg gcacgttgcc ccagcggtcg gcgaaccact gcccgccacc
      361 ggacagactg acacggcctg gcaaaaagcc cggcgcgagg ccggcgtcca gcgcgccgtg
      421 cacgttgccg cgccgcagcg cggacaggaa ccgcacgttc gggagacccg acagcaacgc
      481 ggcggtgcgc agcgtcgcgt gcacggactc cgcgaccgag ccgcgaccga ggacgaccac
      541 gaccggaccg tcgttgccgg cgacgagccg gccgatgccg gagatcgccg agtcggcaat
      601 cggttcgccc ggcagcgtcc tcgcgacgag cgcggcgtgc tcggacaact tgtgcgcgac
      661 cggcgcgcag tcgacgagcg ggaccttcag ctccttcacc gcgcgccgca atcggagcgc
      721 gagcaccggc acggagtctt cgaggccggc gtcgaggatg acgatcgccg ccgcgcggtc
      781 gcagtcggag atctcggcac gcgggagccc gagcaccgcg tcggcgggca agccgtcacc
      841 gagttgcgcg tccacgttgt cggtcccgac gacgcacttc gccaggcgcg accacgcgta
      901 ggcgtcttcg ttggtgccgc gtgcgccgcc gatgaccgcg acggatccgg gaccgtgcag
      961 ttcgatcgac ttcttcagct cggtgacgac gacgtcgagg gcctccggcc acgacgtctc
     1021 gaccagctgg ttctgcacct tgaccgcggg ttcgatgcgg cgcgcgtccg agtgcaccca
     1081 ctcgatgttg tagcggccct tgtcgcacag ccacccctgg ttgaccggct cggagtcgac
     1141 accgaggagg cgcacgagcc ggttcgacga cgactgcaac gcgccgcggc actgcacagc
     1201 gcacgtcgtg cacgacgtct ccgcggtcga gaggtcccag gggcgggcgc ggaagcggta
     1261 ctgcgacgcg gtgagcgctc cgaccgggca gatctgcacc gtgttcccgg agaagtacga
     1321 gtcgaacggc tgccccgcga agttgagcac ctgcatgtgc tcgccgcggt cgaggaaatc
     1381 gatgagggga tcgcccgcga tctcgtcggc gaagcgggtg caacgcccgc actggatgca
     1441 gcgctcgcgg tcgagcaaca cgagcttgct gagcggaatc ggcttctcga agtgccgctt
     1501 ctcctcgacg aatcgcgact cgccgggccc gaacgcgagc gtctggtctt ggagcgggca
     1561 ctcgccgccg cggtcgcaga ccgggcagtc gagcgggtgg ttgatcagca ggaactcgag
     1621 gacgccgtct tggatcttgc gcacgacgtc ggattgcgag tgcgcgacca tcccctccgc
     1681 gacgggcgtg gtgcacgcgg gcgggtaacc gcgcatgccg tcgacctcga cgaggcacat
     1741 gcggcacatg ccgaccggct tcatgcgctc gtgccagcag aaccgcggga tgtagatccc
     1801 gtgctcctgc gcggccttga tcagcagctc gcccggcttc gcctcgaagg tgacgccgtc
     1861 gacggtgacc gtgacggtgt ccttcttcgg ctgctcctgc tcctgcggct gcgtcgcggt
     1921 catacggtga ccccggcgtc ggagttgatg cgggcgatga tctcgtcgcg gaagtacttg
     1981 tcgagactgg cgagcggtga gacgacgctc ggcccgagcg ggcacaacgt cgtctgcgtg
     2041 aacggcgcgg tcgcgacgtt cggggagatg ccggcgccga cgtcgagcag catctcgagg
     2101 tcgccggggc ggccgaggcc gttcgcaatg cggaacagca ccttctcgat ccagcccgtg
     2161 ccctcgcggc agggcgtgca cttgccgcac gattcgtgcg cgtagaactt ggcgagccgc
     2221 cacgcgaccg cgacggggtc ggtcgtctcg tcgaacacca tgatcgcccc cgagccgagc
     2281 gtggtgccga ggttctgctg cacccagtcg atgtcgtagg gcgcgtcgag gtgctcgtcg
     2341 atgccgagca accactggaa cgacgcgccg ccgggcagga agaacttcag ttccttgtcg
     2401 tcgcggatgc cctggccgag gccgtagatg atgtcgcgga acgtgcaacc catctcgacc
     2461 tcgaacacgc cggggttctt gacgtg
//
LOCUS       DASUGA010000472         2142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1403126, whole genome shotgun sequence.
ACCESSION   DASUGA010000472 DASUGA010000000
VERSION     DASUGA010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403126"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VFX21_09280"
     CDS             <1..382
                     /locus_tag="VFX21_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5096194.1"
                     /translation="PFSNPWMSVPMMASAAVLGPPEVPGPGAPGPFAFASDDRVREVL
                     EGSGWHDVEIRELPIEGPMFAGGAHEAASVVVEVSPPYAMALNQRPELHDDLVEVIAG
                     ALEPYEVDGRLAILATEWIVSARA"
     gene            358..1326
                     /locus_tag="VFX21_09285"
     CDS             358..1326
                     /locus_tag="VFX21_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5096195.1"
                     /translation="MDRQRTRVACGNASAPPQATMTRRGWLLFAAMGVIWGTPYLFIR
                     VAAKHLSPPVVVFGRTSLAAVVLVIIAARAGAIRPALERWKPVLAFATIEMAIPWLLL
                     TNAEKKLPSGLTGLLVACVPLVGMFTAYLLGDHHAIRPVRLLGLAVGIGGVAMLVGGD
                     LSGGDGGIPWWSVVQVLLVCVGYATAPFIVERRLGDVPSVGVVAIALSAVALVMAVPA
                     ALTWPDESPPASAWWSVLALAVLCTGIAFIVFFALIHEIGPSRATLITFVNPAVAIIL
                     GAMFLDEEITAATIAGFVFVMAGCWLATRPAPAAALTEETAADITR"
     gene            complement(1369..>2142)
                     /locus_tag="VFX21_09290"
     CDS             complement(1369..>2142)
                     /locus_tag="VFX21_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5096196.1"
                     /translation="KITRSFIAPPVAVALAKHPIVDNYDLSSLEQIFSGAAPLSAELA
                     LEVGKRLGCEVVQGYGMTELSPVSHLTPKGQFKPGSVGVTAPNTETMIVDPATQGPLG
                     IDADGEVWVRGPQVMAGYLNNPKATADTIDRDGWLHTGDIGHIDEDGHLYIVDRLKEL
                     IKYKGFQVAPAELEALLLTHPSVADAAVIGRPDEACGEIPIAFVVLKPDQTCTEDDIK
                     TFVAGQVATYKQLREVHFIDAVPKSASGKILRRVLRDQA"
BASE COUNT          278 a          717 c          762 g          385 t
ORIGIN      
        1 cccgttctcg aacccgtgga tgtcggtgcc gatgatggcg tcggcggcgg tgctgggccc
       61 gcccgaggtt ccggggccgg gcgcgccggg cccgttcgcg ttcgcgtccg acgaccgcgt
      121 gcgcgaagtg ctcgaaggat ccggttggca cgacgtcgag atccgtgagc tgccgatcga
      181 aggcccgatg ttcgcggggg gcgcgcacga ggccgcctcg gtcgtggtcg aagtgtcgcc
      241 gccgtacgcc atggcgctca accagcggcc ggaactgcac gatgacctcg tcgaagtcat
      301 cgccggtgcg ctcgagccgt acgaagtcga cggccggctg gcgatcctcg cgaccgaatg
      361 gatcgtcagc gcacgcgcgt agcctgcggc aatgcatcgg cacccccgca ggccacgatg
      421 acgcgccgcg ggtggctcct gttcgcggcg atgggcgtca tctggggcac gccgtacctg
      481 ttcattcgcg tcgcggccaa gcacctctcg ccgccggtgg tcgtgttcgg gcgcacgtcg
      541 ctcgccgctg tcgtcctcgt gatcatcgcg gcgcgcgcgg gcgcgatccg gcccgcgctc
      601 gagcggtgga agccggtgct cgcgttcgcc accatcgaga tggcgatccc gtggctgctg
      661 ttgaccaacg ccgagaagaa gctgccgtcg ggcctgaccg gcctgctggt cgcgtgcgtg
      721 ccgctcgtgg gcatgttcac tgcgtacctg ctcggtgacc atcacgcgat ccggcccgtt
      781 cggttgctcg gcctcgcggt cggcatcggc ggtgtcgcga tgctggtcgg tggcgacctc
      841 agcggtggcg acggtggcat cccctggtgg agcgtcgtgc aggtcttgct cgtctgcgtc
      901 ggctacgcga ccgccccgtt catcgtcgag cggcgcttgg gcgacgtacc cagcgtcggc
      961 gtcgttgcga tcgcgttgag cgcggtcgcg ctggtgatgg ccgtgcccgc cgcgctcacc
     1021 tggcccgacg aatcgccgcc cgcgtcggcg tggtggtcgg tcctggcgct cgccgtgctc
     1081 tgtaccggca tcgcgttcat cgtgttcttc gcgttgatcc acgagatcgg accttcgcgc
     1141 gcgacgctca tcacgttcgt gaaccctgcg gtcgcgatca tcctcggcgc gatgttcctc
     1201 gacgaggaga tcaccgccgc gaccatcgcg ggcttcgtgt tcgtgatggc cggctgctgg
     1261 ctcgcaaccc ggcccgcacc cgcggccgcc ctcaccgaag agacggccgc cgacatcacc
     1321 cgctaacgct ccggccgccg gcgcaaacgg cggcggagtg acaaggagtc atgcctgatc
     1381 gcggagcacg cggcgcagga tctttcccga agcggacttg gggaccgcgt cgatgaagtg
     1441 gacctcgcgc agctgtttgt aggtcgcgac ctgtccggcg acgaacgtct tgatgtcgtc
     1501 ctcggtgcag gtctggtcgg gcttcaacac gacgaatgcg atggggatct cgccgcacgc
     1561 ttcgtcgggc cggccgatga cggcggcgtc ggcgacgctc gggtgcgtga gcaacagcgc
     1621 ttcgagctcg gccggcgcga cctggaaccc cttgtacttg atgagctcct tgagccggtc
     1681 gacgatgtag aggtgcccgt cttcgtcgat gtggccgatg tcgccggtgt gcaaccaacc
     1741 gtcgcggtcg atggtgtctg cggtcgcctt cgggttgttg aggtagccgg ccatcacctg
     1801 cgggccgcgc acccacacct caccgtcggc atcgatgccc agcggcccct gggtcgcagg
     1861 gtcgacgatc atcgtctcgg tgttcggcgc ggtgacgccg accgaaccgg gcttgaactg
     1921 acccttcggt gtgaggtgcg agaccggtga cagctcggtc atgccgtagc cctgcacgac
     1981 ctcgcagccg aggcgcttgc cgacctcgag cgcgagctcg gccgatagcg gcgcggcgcc
     2041 ggagaagatc tgttcgagcg acgacaagtc gtagttgtcg acgatcgggt gcttcgcgag
     2101 tgcgacggcg accggcggtg cgatgaacga gcgtgtgatc tt
//
LOCUS       DASUGA010000473         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_848222, whole genome shotgun sequence.
ACCESSION   DASUGA010000473 DASUGA010000000
VERSION     DASUGA010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848222"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VFX21_09295"
     CDS             complement(<1..677)
                     /locus_tag="VFX21_09295"
                     /inference="COORDINATES: protein motif:HMM:NF019599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1775 domain-containing protein"
                     /protein_id="HEX5096197.1"
                     /translation="MLRTRRAVAVFVLGVLALTLGATAAFAHTEFDPDEAAPGSVASF
                     TLHVENERSNAGTVKVQLIFMPGDQRITVAALPAVAGWTSTVQDGTVGGAPAKGVTWT
                     RPTGAAGENPEFPITLGPLPDREGPLVFNVVQTYSNGDVVNWEQEEVGGIEPEFPAPI
                     VELRPGAPGSIPSTTIAPSTTVATTTTTVAPTTTTVAPAEDNDDDSTLPLIIGLIALF
                     VAAVGGGV"
     gene            900..1952
                     /locus_tag="VFX21_09300"
     CDS             900..1952
                     /locus_tag="VFX21_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEX5096198.1"
                     /translation="MIRNVIDGMLAAAAKRVEDTGAHVGSGALDAPNFVARMTHTPVR
                     AVRDAIDTAARLEGLPVTADKVRSGELSRAQAQMIANAAAANPAAERALLDKAPEGLR
                     HLRAACNQAIADVEDADERTARQKSLVKLNTWTDPDGLGAGQFHFLPQDFAAFKAVLE
                     AETDRLFREHATAQEHLPREQYAAQALLNLVLGQSESVQRVVKYHVHVMIDHGLLTGR
                     YSHADATCEIPGVGPCDPAWVRSLLDEAVVDYLIKQGKDVTTITTPSRRPTEAMKTVL
                     LAAGMECVIDGCNITGYLELDHVHEVHDGGATCVANLEPKCSQHHDLKTKGWILGPKH
                     PITGKRTLRPPPARAA"
     gene            complement(2086..>2178)
                     /locus_tag="VFX21_09305"
     CDS             complement(2086..>2178)
                     /locus_tag="VFX21_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096199.1"
                     /translation="VNAIPAVCAAEPGIRTYAELPLITAAGFVS"
BASE COUNT          410 a          696 c          728 g          344 t
ORIGIN      
        1 accccaccgc cgacggcggc gacgaagagt gcgatcagac cgatgatcag cggcagcgtg
       61 ctgtcgtcgt cattgtcctc ggccggtgcg accgtggtgg tcgtcggtgc gacggtggtg
      121 gtggtcgttg ccacggtcgt cgacggggcg atcgtcgtag acgggatgga acccggtgcg
      181 ccggggcgca gttcaacgat cggggccggg aattcgggct cgatgccacc gacctcttct
      241 tgctcccagt tgacgacgtc gccgttcgaa tacgtctgca cgacgttgaa gacgagcggg
      301 ccctcgcggt cggggagcgg gccgagcgtg atcgggaact cggggttttc gccggcggcg
      361 ccggtgggac gggtccaggt gacgcccttg gccggcgcac cgccaaccgt tccgtcttgc
      421 accgtcgagg tccagcccgc gacagccggc agcgcggcga ccgtgatgcg ttgatcgccg
      481 ggcatgaaga tgagttgcac cttcacggtt ccggcgttgg aacgctcgtt ttccacatgc
      541 aacgtgaacg acgcgacgct gcccggtgcc gcttcgtcgg gatcgaactc ggtgtgcgcg
      601 aatgctgcgg tcgcgccgag cgtcagcgcg agaacaccga gaacgaagac agcgacagcg
      661 cgccgagtgc gaagcatggt cgggcaacgt agcgcgaacc gatggcgccg agcatgtcac
      721 ctgctcacaa agtttcttcg gagaatcgcg agatttccta ggaaactgtc gtgcccctct
      781 gttatcatac gaacatgtgt ttggggcggc tgcggagtgc cgcgatcgac accgccgacc
      841 atttcgacgc ttctgcgttt ggtccgagcg aggcaattca agccgttgat gatctcgcgg
      901 tgattcgcaa cgtcatcgac ggcatgcttg ccgcggccgc caaacgagtg gaagacactg
      961 gcgcccatgt gggctcgggc gcgctggatg cgcccaattt cgtcgcccgc atgacgcaca
     1021 cgccggtgcg ggcagtacgc gacgcgatcg acaccgcggc caggctcgaa gggttgcccg
     1081 taaccgccga caaggtgcga tccggcgaac tctcccgcgc ccaggcgcag atgatcgcga
     1141 acgccgccgc cgcgaacccg gccgccgagc gcgcgctgct cgacaaagca ccggaagggt
     1201 tgcggcatct ccgggcggcg tgcaatcaag cgatcgcaga cgtcgaagat gccgatgaac
     1261 gaacggcacg ccagaaatcg ttggtgaagt tgaatacgtg gaccgacccc gacgggctcg
     1321 gcgccgggca gttccacttc ctgccccagg acttcgcggc gttcaaggca gtccttgaag
     1381 cggagaccga tcggttgttc cgggaacatg ccacggcgca ggagcatttg ccgcgcgagc
     1441 aatacgcggc tcaagcactc ctcaacctgg tgctcggcca atcggagtcg gtgcagcggg
     1501 tcgtgaagta ccacgtccac gtgatgatcg atcacgggct gctgaccggg cgctactcgc
     1561 atgcggacgc gacgtgcgag atccctgggg ttgggccgtg cgatccggcg tgggtccggt
     1621 cgctgctcga cgaagcggtc gtcgactacc tgatcaagca gggcaaagac gtcacgacca
     1681 tcacgacgcc gagccgccgg cccaccgaag cgatgaaaac cgtgctcttg gcggcgggca
     1741 tggagtgcgt gatcgatggt tgcaacatca ccggctacct cgaactcgac cacgtgcacg
     1801 aagtgcacga tggcggcgct acctgtgttg cgaacctcga accgaaatgt tcgcaacacc
     1861 acgatctgaa aaccaagggc tggatcctcg gccccaaaca cccgatcacc ggaaaacgca
     1921 cccttcgacc accacccgca cgcgcggcgt gaccccggcg gacgaagcaa gagcgtttcg
     1981 acaagcaatg ccatggcgtg ccgacgacca tctcgcggac caaggcgcca gatcgaccct
     2041 tgacataccg ccgcgcgcca aacgcgcgcg gcgggcagat cagtatcacg acacgaagcc
     2101 cgcggccgtg atgaggggca gctcggcgta cgtgcggatg cccggttcgg ccgcgcacac
     2161 ggcgggaatg gcgttcac
//
LOCUS       DASUGA010000474         3998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1849992, whole genome shotgun sequence.
ACCESSION   DASUGA010000474 DASUGA010000000
VERSION     DASUGA010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849992"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..2130)
                     /locus_tag="VFX21_09310"
     CDS             complement(139..2130)
                     /locus_tag="VFX21_09310"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX5096200.1"
                     /translation="MRRRVLDALRSLRGNDRRSRLLQLAAVFGAYYLVAELGLRLSLV
                     ERTITPLWPPTGVALVACLSYGRRVWPAVAIAAFAVNAPLGDSLATAAGIAVGNTLAP
                     VVAATLLVRVDFSPRLQRVRDALTLVFLGALVSMAISATIGTLTLLASDAIPAHRFAG
                     SWLVWWTGDAMGVVIVAPFLWSLAIDGPRLRRAERLEAIVAALVLVGVWAIAFLSTVH
                     PMFLLPPFVGWIAWRFQQRGAAPAALVVSVLATWAAVEEVGPFEHASLVDRMVVLQSF
                     NAIVALTSIVTAAAISQRQRVAEREHRVIETLQRSLLPERLPDVPGVDVAARYMPASH
                     DVALGGDWYDVIPLPDGRVGLGVGDVAGHGVGAAASMGQLRMALRAYALEELEPSDVI
                     GRLNRVLRELHPNAMATLWYGNYDPATRELRFASAGHPPPMLVDARRTARYIEAPNGP
                     PLGVVHQLACSDASLHLDVGTTILLYTDGLVERRRASIDEGLNALSERVRQAPAELEA
                     LCDHVMDGLTTAPTDDVALLAIRPTALVGTQLRLRRPAVLAGVPEARHMMRRWLEHNH
                     VASDDAFDALVAATEAYTNVVQHAYGLQVGAVEVDALIGDGRLEIAIRDRGRWRGGES
                     LRDDGGGRGLLMMRTLMDTVEIDSDQQGTEVRMRRALKT"
     gene            complement(2219..3355)
                     /locus_tag="VFX21_09315"
     CDS             complement(2219..3355)
                     /locus_tag="VFX21_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX5096201.1"
                     /translation="MPRKRHTLAPDDAGGYLAEELLGLEGFAQESSLLYHRHSPSAIV
                     AADAVAEPTPRAVTSDHPLQPRHLRTSKLGPGTDAVLGRVVIMGNDNVTIAWCAATRD
                     SELYRNALGDELVYVQQGTALLETSFGTLAVGKGDYVHIPCATTHRWRVHEPPLEALI
                     LEARGHVRVPRKYVTDRGQLREGAPYSERDLRPPDGPLVCEGEDVAVLVRNRGGLTQL
                     VHAHHPLDVVAWDGCAWPFAFSIYDFEPVVGARHQPPPVHQTFEGDGFVVCSFVPRPF
                     DFGVDAVKIPYHHSNVDSDEVLFYAGGDFMSRAGAGIEEGSMTYHPAGFVHGPQPGSL
                     EASMDADHTNETAVMIDTFRPLSLSDAARTVSDDNYPWTWSGGR"
     gene            complement(3382..>3998)
                     /locus_tag="VFX21_09320"
     CDS             complement(3382..>3998)
                     /locus_tag="VFX21_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019927501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX5096202.1"
                     /translation="FEDHVFGVLLLNDWSARDIQAFEYQPLGPFLGKSFATSVAPWVV
                     PLDALAPFMVPGWVPRQDPEPAAYLRDTTPHMPALRFEVLLTSQQMRAAGCAPVVVST
                     VALEEALYWSMPQQLAHATVNGASVRTGDVFATGTLSGPDPMTQAGSLIERTWRGECP
                     LVLPTGETRAFLADGDEVTLRGWCGAGTDRVDLGEVTGVVRGGR"
BASE COUNT          630 a         1410 c         1383 g          575 t
ORIGIN      
        1 ccagttgcag gcattcgacg catccgtcac ccgacggcgc gacgtccttg atctggtcgg
       61 tgtgggtaca actgtcggcc atgcgcgcca gtgtgcccga gttcgccacg cgatacccag
      121 acgggcaggt catcgcgctc aggtcttgag tgcgcggcgc attctgactt cggtgccctg
      181 ttggtcggag tcgatctcaa cggtgtccat cagcgtgcgc atcatcagca gtcctcgacc
      241 gccgccgtcg tcccgcagcg actcaccgcc gcgccatcgg ccccggtcgc gtatcgcgat
      301 ctcgagtctg ccatcgccga tcagcgcgtc gacttccacc gctccgacct ggaggccgta
      361 cgcgtgctgc accacgttgg tgtatgcctc cgttgcggcc acgagcgcgt cgaacgcgtc
      421 atccgacgcg acgtggttgt gctccagcca gcgccgcatc atgtgccgtg cttcgggcac
      481 gccggccagc accgccgggc ggcggagacg cagctgcgtg ccgacgagcg ccgtgggccg
      541 gatcgcgagc agcgcgacgt cgtcggtcgg cgcggtcgtg aggccgtcca tgacgtggtc
      601 gcacagcgcc tcgagctccg cgggcgcctg gcgtacgcgc tccgacagcg cgttgaggcc
      661 ctcgtcgatc gacgcccgcc gccgctccac gaggccgtcg gtgtagagca ggatcgtcgt
      721 gccgacgtcg aggtggagtg acgcgtcact gcacgcaagc tgatgcacga cgccgagcgg
      781 tggcccgttc ggcgcctcga tgtagcgcgc cgtacgacgg gcgtccacga gcatgggtgg
      841 tggatgcccc gcgctcgcga accgcagctc acgcgtcgca gggtcgtagt tgccgtacca
      901 cagcgttgcc atcgcgttgg ggtgcagctc ccgcaagacg cggttgagcc gcccgatcac
      961 gtcgctcggt tcgagctcct cgagggcgta cgcgcgcaac gccatgcgca gctgacccat
     1021 cgacgcggcc gcgccgacac cgtggcccgc gacgtcgccg accccgagcc cgacccgacc
     1081 gtcgggcagc ggtatgacgt cgtaccagtc gccgcccagc gcgacgtcgt gcgacgccgg
     1141 catgtagcgc gccgcgacgt cgacacccgg cacgtcgggc aagcgttcgg gcagcaggct
     1201 ccgttgcagc gtctcgatca cgcggtgctc gcgctcggcg acccgctggc gctgcgagat
     1261 cgcggccgcg gtgacgatcg aggtcagtgc gacgatggcg ttgaagctct gcaagacgac
     1321 catgcggtcg accaacgagg cgtgctcgaa tggtccgacc tcttcgaccg cggcccacgt
     1381 cgcgagcacc gagacgacca gcgccgcggg cgccgcgccg cgttgttgga accgccacgc
     1441 gatccagccg acgaagggcg gcagcaggaa catcgggtgc accgtcgaca ggaaggcgat
     1501 cgcccacacg cccaccagca ccagcgccgc gacaatggct tcgaggcgtt ccgcgcggcg
     1561 gaggcgcggc ccgtcgatcg ccaacgacca caagaagggc gcgacgatga cgacgcccat
     1621 ggcatcaccc gtccaccaga cgagccagct gccggcgaag cggtgcgccg ggatcgcgtc
     1681 cgacgcgagc agggtgagcg tcccgatcgt ggcgctgatc gccatgctca ccagcgcgcc
     1741 gaggaacacg agcgtgagcg cgtcccggac acgctgcaag cgcgggctga agtcgacgcg
     1801 caccagcaac gtggcggcga cgaccggtgc gagcgtgttg ccgaccgcga tccccgccgc
     1861 cgtcgccagc gagtcaccga gcggtgcatt caccgcgaag gcggcgatcg cgacggcggg
     1921 ccacacccgc cggccgtacg agaggcacgc gacgagcgcg acgccggtcg gcggccacaa
     1981 cggtgtgatc gtgcgctcga cgagtgagag ccggaggccg agctcggcga ccaggtagta
     2041 cgcgccgaag acggctgcga gctgcagcag tcggctccgc cgatcgttgc cccgtaggga
     2101 acgaagcgcg tccagcaccc gtcgtcgcac cggtgacact ccctcgcatc gtcaccctgc
     2161 ccgccaggtt ggatgatggc tgacaaacgg tccggtgtcg accggtcttc tcggtttctc
     2221 agcgaccgcc cgaccacgtc cacgggtagt tgtcgtccga gacggtgcgc gccgcatccg
     2281 acagcgacaa cggccggaac gtgtcgatca tcaccgcggt ctcgttcgtg tggtcggcgt
     2341 ccatcgacgc ttcgaggctc ccgggctgtg gcccgtgcac gaatccggcc gggtgatacg
     2401 tcatcgatcc ctcttcgatg cccgcaccgg cccggctcat gaaatcgccg ccggcataga
     2461 agaggacttc gtcggagtcg acgttcgagt ggtggtacgg gatcttcacc gcgtcgacac
     2521 cgaagtcgaa cggccgcggc acgaacgagc acaccacgaa gccgtccccc tcgaacgtct
     2581 ggtggaccgg cggcggctga tgccgcgccc ccacgaccgg ctcgaagtcg tagatcgaga
     2641 acgcgaacgg ccacgcacat ccgtcccacg cgacgacgtc gagcggatgg tgcgcgtgca
     2701 cgagctgcgt cagcccaccg cggttgcgca ccaagacggc gacgtcttcg ccctcgcaca
     2761 cgagcggccc gtcgggcgga cgcaggtcgc gctccgagta cggcgcacct tcgcggagtt
     2821 ggccgcgatc ggtgacgtac ttgcgtggga cgcggacgtg cccgcgcgct tcgaggatga
     2881 gcgcttccag cggcggctcg tggactcgcc accggtgcgt cgtcgcacac gggatgtgga
     2941 cgtagtcgcc cttgccgacc gcgagtgtcc cgaagctggt ctcgagcagc gcggttccct
     3001 gctgcacgta gaccagctcg tcgccgagcg cgttgcggta cagctcactg tcgcgcgtcg
     3061 ccgcacacca cgcgatcgtc acgttgtcgt tccccatgat cacgacgcgg cccagaacgg
     3121 cgtcggtgcc ggggccgagc ttcgacgtac ggaggtgacg cggctgcaac ggatgatcgg
     3181 acgtgaccgc gcggggcgtc ggctcggcga ccgcgtcggc ggcgacgatc gcgctcggcg
     3241 agtgccggtg gtacagcaac gacgattcct gcgcgaagcc ttcgagtccg agcaactcct
     3301 cggcgaggta cccgccggcg tcgtcgggcg ccagcgtgtg acgcttccgc ggcaccggac
     3361 cacaacgcat gtagtgcggc atcatcgacc tcctcggacg acgccggtga cctcgccgag
     3421 atctacccgg tcggtaccgg caccgcacca tcctcgcagc gtcacctcgt cgccgtcggc
     3481 caggaacgcg cgcgtctcgc cggtcgggag tacgagtggg cactccccgc gccacgtgcg
     3541 ttcgatgagg ctgccggcct gggtcatcgg atcgggcccc gacaacgtgc cggtcgcgaa
     3601 gacgtcgccg gtccggacgg acgcgccgtt gacggtggcg tgcgcgagct gttggggcat
     3661 cgaccagtac agcgcctcct ccaacgcgac cgtgctcacg acgacgggtg cgcagccggc
     3721 ggcgcgcatc tgttgtgacg tgagcagcac ttcgaaccgc agcgcgggca tgtgcggcgt
     3781 ggtgtcccgc aggtaggcgg ccggctcggg gtcttgacgg ggcacccaac ccggaaccat
     3841 gaacggcgcg agcgcgtcga gcggcaccac ccacggtgca acggatgtcg cgaacgactt
     3901 gccgaggaag gggccgagcg gctggtactc gaaggcctgg atgtctcgcg ccgaccagtc
     3961 gttgaggagc agcacgccga acacgtgatc ttcgaacc
//
LOCUS       DASUGA010000475         2633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_300292, whole genome shotgun sequence.
ACCESSION   DASUGA010000475 DASUGA010000000
VERSION     DASUGA010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300292"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VFX21_09325"
     CDS             <1..438
                     /locus_tag="VFX21_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096203.1"
                     /translation="TLTGWAPRAPVLARRLAAYACGATLAVQPAIAHAATADVPVVRA
                     PATATTTTSAPAPEIAPEPAVTDAPAPPPVATPVSANYLVAPGDNLWRIAAAELTRRT
                     GATPDEDAIARYWRAVVDANRSTLRSGEPSLIYPGELVVLPAP"
     gene            482..1381
                     /locus_tag="VFX21_09330"
     CDS             482..1381
                     /locus_tag="VFX21_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5096204.1"
                     /translation="MALLDGKVAIVTGAGRGIGRGHARLLAAHGAAVVVNDLGAAMDG
                     SDEASQAQQVVDEIVADGGKAIADGGNVATWKGAEALVGRAVDEFGRLDILVNNAGIL
                     RDAMSFNITEEQWDSVLTVHLKGHMGMSHFAAAHWRERGKAGDDVSGRIINTASESGL
                     FGQAGQINYATAKAGIVGMTLVLARELKKYNVTANVICPRALTRMTSSVPGAEDFMSG
                     PEWDPEQIAPVVGWLASDAAADISGQVFIVWGTKVHLIEGFHLVGTLDRGEGRFTPDE
                     LIARKDELFGGRRSKVPPMGIGQ"
     gene            1384..>2633
                     /locus_tag="VFX21_09335"
     CDS             1384..>2633
                     /locus_tag="VFX21_09335"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX5096205.1"
                     /translation="MPEPPDKPEGPLLVDQLRFMAQRWPDEIAYRNLGTATQITFAAW
                     DKRSNQVARWLAEHGVGKGDRVALYMDSDHCLEWIVTYAGIHKAGAVMVPVNTRLAVD
                     EVLTILTHAEPRVVVTNARLAENTRAVEQQLDIAGVLSVGAQGSHQDFTVVDAFDDSA
                     TQVPLDADDMADIMYTSGTTGLPKGVLVRHRNVAMMPNAKPHWTGAGWLHGAPLFTFA
                     GMSFIYNPMKMGLAGLYMPKFDVDRWFDVVERDRPSMIFLVPAMAELVTASPRFEAAD
                     LSSPFAVSIGSAPLAPATLKRLQDRMPQASVSNSYGLTEAGPAFIVMPKGDLDRKPGS
                     VGKAMPPLEVKVVDPDSDEPKEPNEVGELLVRLRGKRREYYKDDGATSATWTDDGWLR
                     TGDLAYLDDEDFVYISGRIKDMIIR"
BASE COUNT          447 a          940 c          891 g          355 t
ORIGIN      
        1 accctcacgg gctgggcgcc gcgcgccccc gtgctcgcgc gccggctggc ggcgtacgcg
       61 tgcggcgcca cgctcgccgt gcagcccgcg atcgcgcacg cggcgacggc tgacgtcccc
      121 gtcgtgcgcg caccggcgac cgcgaccacc acgacgtcgg cacccgcacc cgagatcgcg
      181 cccgaacccg ccgtcaccga cgcgccggca ccaccgcccg tcgcgacacc cgtcagcgcg
      241 aactacctcg tcgcgccggg cgacaacttg tggcgcatcg ccgccgccga gctcacgcgc
      301 cgaaccggcg cgacacccga cgaagacgcg atcgcgcgct actggcgcgc ggtcgtcgac
      361 gccaaccgct cgacgctgcg gtcgggcgag ccgagcctca tctatcccgg tgaactggtc
      421 gtcctgcccg cgccgtgagg cccggttgac ctgacacgag cgtcagggtc agactcggcc
      481 gatggccttg ctggacggaa aagtcgcgat cgtcaccggc gcgggacgcg gcatcggacg
      541 cgggcacgcg cgcttgctcg ccgcgcacgg cgcggccgtc gtcgtcaacg atctcggcgc
      601 cgcgatggac ggcagcgacg aggcgtccca ggcccaacag gtcgtcgacg agatcgtcgc
      661 cgacggcggg aaagcgatcg ccgacggcgg caacgtcgcc acgtggaagg gcgccgaggc
      721 gctcgtcggc cgggccgtcg acgagttcgg ccgcctcgac atcctcgtca acaacgcggg
      781 catcctgcgc gatgcgatga gcttcaacat caccgaggag caatgggact cggtgctcac
      841 cgtgcacctg aaggggcaca tgggcatgtc gcacttcgcc gccgcgcact ggcgtgagcg
      901 cggcaaggcc ggcgacgacg tcagcggccg catcatcaac accgcgagtg agtcgggact
      961 gttcgggcag gcgggccaga tcaactacgc aactgcgaaa gccggcatcg tcggcatgac
     1021 gctcgtgctc gcgcgcgaac tgaagaagta caacgtcacc gcgaacgtca tctgcccgcg
     1081 cgccttgacc cgcatgacgt cgagcgtgcc cggcgcggaa gacttcatga gcggtccgga
     1141 gtgggacccc gagcagatcg cgcccgtggt cggctggctc gcgagcgacg ccgcggccga
     1201 catctccgga caggtgttca tcgtgtgggg caccaaggtg cacctcatcg agggcttcca
     1261 cctcgtcggc acgctcgacc gcggcgaagg ccgcttcacg cccgacgaac tgatcgcgcg
     1321 caaggacgag ctgttcggcg gccgccgttc gaaggtgccg ccgatgggca tcgggcaata
     1381 gccgtgcccg agccgcccga caaacccgag ggcccactcc tcgtcgacca actgcgcttc
     1441 atggcgcagc gatggcccga cgagatcgcg taccgcaacc tcgggacggc gacgcagatc
     1501 acgttcgcgg catgggacaa gcgctcgaac caggtcgcgc gctggctcgc cgagcacggc
     1561 gtcggcaagg gcgaccgcgt cgcgctctac atggacagcg atcactgcct cgaatggatc
     1621 gtcacctacg cgggcatcca caaggccggg gccgtcatgg tgccggtgaa cacgcggctc
     1681 gcggtcgacg aagtcctcac gatcctcacg cacgccgagc cgcgtgtcgt cgtcacgaac
     1741 gcgcgcctgg ccgagaacac gcgcgcggtc gaacaacagc tcgacatcgc cggtgtgctc
     1801 agcgtcggcg cgcagggatc gcatcaggac ttcaccgttg tcgacgcgtt cgacgacagc
     1861 gctacgcagg tgccgctcga cgccgacgac atggccgaca tcatgtacac gtcgggcacg
     1921 accgggctgc cgaagggtgt gctcgtccgg caccgcaacg tcgcgatgat gccgaacgcg
     1981 aaaccgcact ggaccggcgc gggctggttg cacggcgcgc cgctgttcac gttcgcgggc
     2041 atgagcttca tctacaaccc gatgaaaatg ggcctggccg gtctctacat gccgaagttc
     2101 gacgtcgacc gctggttcga cgtcgtcgag cgcgacaggc cgtcgatgat cttcctcgtg
     2161 cccgcgatgg ccgagctcgt caccgcgagt ccgcgcttcg aagccgccga cctgtcgtca
     2221 ccgttcgcgg tgtcgatcgg cagcgcgccc ctcgcgcccg cgacgctgaa gcgcttgcag
     2281 gaccggatgc cgcaggcgtc ggtgtcgaac tcgtacggcc tcaccgaagc gggtccggcg
     2341 ttcatcgtga tgcccaaggg tgacctcgac cgcaagccgg gctccgtcgg taaggcgatg
     2401 ccgccactcg aggtgaaggt cgtggatccc gacagcgacg agccgaagga gccgaacgaa
     2461 gtcggcgagt tgctcgtgcg cctgcgcggc aagcggcgcg agtactacaa ggacgacggc
     2521 gcgacgtcgg caacgtggac cgacgacggc tggctgcgca ccggcgacct cgcgtacctc
     2581 gacgacgaag acttcgtgta catctccggc cggatcaagg acatgatcat ccg
//
LOCUS       DASUGA010000476         2808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_796981, whole genome shotgun sequence.
ACCESSION   DASUGA010000476 DASUGA010000000
VERSION     DASUGA010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796981"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1939)
                     /locus_tag="VFX21_09340"
     CDS             complement(<1..1939)
                     /locus_tag="VFX21_09340"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5096206.1"
                     /translation="MQSFLQFLIIGLGAGASYALFAHGAVLIYRGSGLVNFAQGAVGT
                     LAAYIAFVDLVEKRSWDTWPAIAVGVVAAGIAALLFQATVLRALRRAAPIVRVIATIA
                     LLGLLQAVVAKRYGAGNQSVDHFLPDTVWKWGDIAVQEERVIIFVIAVVVTVGLWAWT
                     RFTRVGLAINASAQNERAVQTLGWSPDRLAALTWTIGGILGGLAAVLAAPLTGLSATT
                     FTVVVTVAGLGAALLGGFQSFPLTLLGGLIIGVGEAMATLYGNDIQDFFHQDLISGLN
                     RMPAFLVILIVVVIRGRGLPLRSHVAERLPKLGTGQISVRAVLLASALTAVVLFGVMD
                     EAWAQATYTSLATAIMVLSIVVLTGYAGQISLAQWALAGIGALIAGRFVRADVPIELA
                     IVLGILLTIPVGLIFAVPALRTRGVNLAVVTLGLGFLVSEVVFANPNYLGSPLDGGTK
                     IGRVKLFGMKVDAFDHPHAWAAVSLIAFVLLALLVANLRRSRTGRRLIAVRTNERAAA
                     SLGISVFSVKLYAFAVSAALAAVAGILIGFRGQVVNYTEFNIFASINTLGNAVIGGLG
                     YVLGAVFAAPMAINGLSTRVIKDWINLGGQWDLIIGSVIVFVILIVHQNGVADVAVHG
                     RRVWETLHLVRKHRVRDPLPPA"
     gene            complement(2018..>2808)
                     /locus_tag="VFX21_09345"
     CDS             complement(2018..>2808)
                     /locus_tag="VFX21_09345"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5096207.1"
                     /translation="QALIDQDLLKIGAIRVDLAAASALVGLLGDLYEDQGATFPYDVP
                     VPAGTTDYTQFILGAQQAGVDGVTMSLGEQEAVQVAKAGQQLNSDLPIATSLGTFPHS
                     SVADLGDYADKMLFVGAFPPATVEGIPVYQALLDDLASTGNEQLQPENLKTSPMRSWI
                     GLYALLKMIRDAGMTDFTREGITQMLHDAKDVPMLGMYGDENWTPNLDHPGIFKRAGM
                     NHWAVYKWNPDAKSIAGDGNFEEVSTMSFDEVLCGSPFGAPEPC"
BASE COUNT          507 a          945 c          944 g          412 t
ORIGIN      
        1 ccgcgggcgg caacgggtcg cgcacgcggt gcttgcgaac gagatgcagc gtctcccaca
       61 cccgccgacc gtgcaccgcg acgtcggcaa caccgttctg gtgcacgatc aggatgacga
      121 acacgatgac ggagccgatg atgaggtccc attggccacc gaggttgatc cagtccttga
      181 tgacacgcgt cgagagtccg ttgatcgcca tgggcgccgc gaacacggcg ccgagcacat
      241 acccgaggcc accgatcacc gcgtttccaa gcgtgttgat cgaggcgaag atgttgaact
      301 cggtgtagtt gacgacctgc ccgcggaacc cgatcaggat gccggcgacc gcggcgagcg
      361 cggcggagac ggcgaacgca tacagcttca cgctgaacac cgagatgccc agcgacgccg
      421 cggcccgttc gttggtgcgc accgcgatca accggcgacc ggtgcgtgac cggcgcaggt
      481 tggcgaccag caacgcgagc aggacgaacg cgatcaagct caccgcggcc cacgcatgcg
      541 ggtggtcgaa cgcgtcgacc ttcatgccga acagcttcac ccgcccgatc ttcgtcccgc
      601 cgtcgagcgg gctgcccagg tagttggggt tggcgaacac cacttccgac acgaggaagc
      661 cgagcccgag ggtgacgacc gcgaggttta cgccgcgggt tcgcagtgcg ggcaccgcga
      721 agatcagccc gacgggaatc gtcagcagga tgccgagcac gatcgcgagc tcgatcggca
      781 cgtcggcgcg cacgaagcga ccggcgatca aggcgccgat gccggccagg gcccactgcg
      841 cgagcgagat ctgaccggcg taccccgtga gcaccacgat cgacagcacc atgatcgcgg
      901 tggccaagga tgtatacgtc gcttgcgccc acgcttcgtc catgacgccg aacagcacga
      961 cggcggtcag cgccgacgcg agcaggaccg cgcgcacgct gatctggccc gtgccgagct
     1021 tcggcagtcg ctcggcgacg tgcgaccgca acgggaggcc gcgcccacgg atgacgacca
     1081 cgatcaggat cacgaggaac gcgggcatgc ggttgagccc gctgatgaga tcctggtgga
     1141 agaagtcctg gatgtcgttg ccgtacagcg tcgccatcgc ttcgccgacg ccgatgatca
     1201 gcccgccgag caacgtgagc gggaacgact ggaacccgcc gagcaacgcc gcaccgagac
     1261 cggcaaccgt gacgaccacg gtgaatgtcg tcgccgagag accggtgagc ggcgcggcca
     1321 gcacggccgc gagcccaccg aggatgccgc cgatcgtcca cgtcagcgcc gcgagccggt
     1381 cgggcgacca tcccagtgtc tgcaccgcgc gttcgttctg ggcgctcgcg ttgatcgcga
     1441 ggccgacgcg ggtgaaacgg gtccacgccc acagcccgac ggtgacgacc accgcgatca
     1501 cgaagatgat cacgcgctcc tcttggaccg cgatgtcacc ccatttccac accgtgtcgg
     1561 gaaggaagtg gtcgacggac tggttgccgg ccccgtagcg cttggcgacg accgcttgca
     1621 gcaggccgag gagcgcgatc gtcgcgatca cccgcacgat cggcgccgcg cgtcgcagcg
     1681 cgcgcagcac cgtcgcctgg aacaggagcg cggcgatacc ggccgcgacg acaccgaccg
     1741 cgatcgcggg ccaagtgtcc cacgaacgct tctcgacgag gtcgacgaac gcgatgtacg
     1801 cggccaacgt gccgaccgcg ccctgcgcga agttgacgag tccggagccg cggtagatga
     1861 gcaccgcgcc gtgtgcgaac agcgcatagc tggcgccggc accgagcccg atgatcaaga
     1921 actgcaggaa cgattgcacc gcgtctactg gccttcctgg tcgaccggga accggtgggc
     1981 gacgggtcgg tgcccgtcgc ccactcggtt catccgatca gcagggttcc ggagcaccga
     2041 agggcgaacc gcagagcacc tcgtcgaagc tcatcgtcga gacctcctcg aagttgccat
     2101 cgccggcgat gctcttcgcg tcggggttcc acttgtagac cgcccagtgg ttcatgcccg
     2161 cccgcttgaa gatgcccggg tggtcgaggt tcggcgtcca gttctcgtcg ccgtacatcc
     2221 ccaacatcgg gacgtccttc gcgtcgtgca gcatctgcgt gatgccttca cgcgtgaagt
     2281 cggtcatgcc cgcgtcgcgg atcatcttga gcaacgcgta gagaccgatc cacgaacgca
     2341 tcggactggt cttgaggttc tccggctgca gctgttcgtt gcccgtcgaa gcgaggtcgt
     2401 cgagcaacgc ttggtacacc ggaatgcctt cgacggtcgc gggcgggaag gcgccgacga
     2461 acagcatctt gtccgcgtag tcgccgaggt cggcaacgct gctgtgcggg aacgtgccca
     2521 ggctggttgc gatcggcagg tcactgttca actgctgccc ggccttggcg acctggacgg
     2581 cttcctgctc gccgagcgac atcgtcacac cgtcgacacc ggcctgctgg gcgccgagga
     2641 tgaactgcgt gtagtcggtc gtcccggccg gcacgggcac gtcgtacggg aacgtcgcgc
     2701 cttggtcctc gtagaggtca ccgagcaggc cgacgagcgc ggacgcggcc gcgaggtcga
     2761 cacggatcgc gccgatcttc agcaggtcct ggtcgatgag tgcctgcg
//
LOCUS       DASUGA010000477         2889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_708835, whole genome shotgun sequence.
ACCESSION   DASUGA010000477 DASUGA010000000
VERSION     DASUGA010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708835"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1949)
                     /locus_tag="VFX21_09350"
     CDS             complement(<1..1949)
                     /locus_tag="VFX21_09350"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HEX5096208.1"
                     /translation="MPVDLGATTPWRRTAMFPEYRDRSLLDVFRELATTCVDRIAVRD
                     GERSLSYRELYDRIHAIARGLRSSRASTRAAGDAPWVVTGVVGHGIDALLTVYGVMAA
                     GAVLVPIDAAEPVERMRLIHREGGAELAVADRAHAELARQATRSRTLLLDDLMRDPGL
                     PSDPEPEPGTLAMINFTSGSTGTPKGVVRDHDTLVRAGFTTAESNLIEADDVVAFIGS
                     FSFIGAYARSFGAFVAGAELCMHDQRRGASRELAEWILDRRISVLQFIPSVLRNFTEA
                     VARAGVPRLDSVKIVSLGGETTYGRDVARARAVFGTDTRIVNRYGSSETSVLAEWITT
                     PEDDARADESLPLGHALPWAEIVVVDDAGEPLESGAVGTPAVLSEHCSLGYWNDPELT
                     AARFWTLPDGRRGFRMTDRVRSRDDGALEYVARGDDRVKVRGTMVSPSEIERALTALA
                     GVSEAAVVPAPARDGGTRLVGYIVPEARANVSGWELRRALAATMPSAMVPATIVTLES
                     MPLTPRGKVDRRALPPAQDPESQPYREPLGQEGDLALIFSRVLGVERVGRDDDFFELG
                     GDSLGVIELVAEIAERFSVDVPASTVLEAPTVSSLFARLQHRRSRHASPVVPLRTDAS
                     EPPLFCVTGGGAPAISMRALSAAMPT"
     gene            complement(1951..2679)
                     /locus_tag="VFX21_09355"
     CDS             complement(1951..2679)
                     /locus_tag="VFX21_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096209.1"
                     /translation="MDTIAIVDHTRKHRPEPGVLDAIAEALTTQIERDFAPAWKVKPR
                     RVTVGGRGDKLHLFDTSHQADDYGWHIVDGHGDPYAHLFAGVSIKRGSTWVDGEDAIS
                     VTASHEVLEMLVDPAANEYTFNGLRTLWSREVCDPVQADAYRIVASGMRVAVSNFVLP
                     AFFNPWAPGPYDRLGVLTEPFSIARGGYAIVERATRTREREARTIGVRFDRAVPKWQR
                     TQKLEGWGRTFWRLALVAGSSRRS"
BASE COUNT          450 a         1027 c          976 g          436 t
ORIGIN      
        1 gtgggcatcg cggctgagag cgcgcgcatc gagattgccg gtgcaccgcc gccggtcacg
       61 cagaacaacg gcggctcgct cgcgtcggtg cgcaacggca cgaccggcga cgcgtgccgc
      121 gaacggcggt gttggaggcg cgcgaacagg gaggacaccg tcggcgcctc gagcaccgtc
      181 gaggccggga cgtcgaccga gaatcgctcg gcgatctcgg ccacgagctc gatcacaccg
      241 agcgaatcgc caccgagctc gaagaagtcg tcgtcgcgcc cgacgcgttc cacaccgagc
      301 actcgcgaga agatcagcgc gaggtcgcct tcctggccga gcggctcgcg gtacggctgc
      361 gattcgggat cctgcgccgg cggcagcgcg cgccggtcga ccttcccgcg cggggtgagc
      421 ggcatcgact ccagcgtgac gatcgtcgcg ggaaccatcg cgctcggcat cgtcgccgcc
      481 aacgccctcc ggagctccca cccactgacg ttggcacggg cctcgggaac gatgtacccc
      541 acgaggcggg tcccgccgtc gcgcgcgggc gccggcacca ccgcggcctc ggagacaccg
      601 gcgagcgcag tcaacgcacg ctcgatctcg ctcggactca ccatcgtccc gcgcaccttc
      661 acgcggtcgt cgccacgcgc cacgtactcg agcgcgccgt catcccgact ccgcacgcgg
      721 tcggtcatgc ggaagcctcg acgaccgtcg ggcagggtcc agaacctcgc cgcggtcagc
      781 tccgggtcgt tccagtatcc gagcgaacag tgctcgctga ggaccgcggg cgtcccgacc
      841 gcacccgact cgagcggctc gccggcgtcg tcgaccacga cgatctcggc ccacgggagc
      901 gcatgcccga gcgggagcga ctcgtcggcg cgggcgtcgt cctccggagt cgtgatccac
      961 tctgcgagca cgctcgtctc gctcgagccg taccgattga cgatgcgcgt gtcggttccg
     1021 aacacggctc tcgcgcgtgc gacgtctcgt ccgtacgtgg tctcgccgcc gagtgagacg
     1081 atcttcaccg agtccaggcg gggcacgccg gctcgggcca ccgcctccgt gaagttgcgc
     1141 agcaccgacg ggatgaactg cagcaccgag atccggcggt cgaggatcca ctccgcgagc
     1201 tcacgactcg cgccgcggcg ctggtcgtgc atgcagagct ccgcccccgc gacgaacgcg
     1261 ccgaacgagc gcgcgtaggc accgatgaac gagaaggagc cgatgaaggc gaccacgtcg
     1321 tccgcctcga tgagattgct ctcggccgtg gtgaatcccg cccgcaccag cgtgtcgtgg
     1381 tcgcgcacga ctcctttcgg tgtgccggtc gatcccgacg tgaagttgat catcgccagg
     1441 gtccccggtt cgggctcggg gtcggacggc aatccggggt cgcgcatcag gtcgtcgagc
     1501 agcaacgtgc gggatctcgt tgcctgccgg gccagctcgg catgggcacg atcggcgacg
     1561 gcgagctcgg cgccgccttc acgatggatc agccgcatcc gctcaacggg ctcggccgcg
     1621 tcgatcggca cgagcaccgc acccgcggcc atcaccccgt agacggtgag caacgcgtcg
     1681 atgccgtgac cgacgacgcc ggtgaccacc cacggcgcat cgccggctgc tcgcgtcgat
     1741 gcccgcgagc tgcgcagccc gcgcgcgatc gcgtggatgc ggtcgtacag ctcgcgatac
     1801 gagagcgaac gctcgccgtc gcgtaccgcg atgcgatcca cgcacgtggt cgcgagctcg
     1861 cggaacacgt ccagcagcga acgatcccga tactccggga acatcgccgt gcgacgccag
     1921 ggtgtggttg ctccgagatc gaccggcacg tcagctccga cgcgaagatc ctgcgacgag
     1981 cgccaagcgc cagaacgtgc ggccccagcc ttcgagcttc tgtgtgcgct gccacttcgg
     2041 aaccgcgcgg tcgaagcgca caccgatcgt tcgcgcctcg cgctcccgcg tgcgcgtcgc
     2101 gcgttccacg atggcgtagc caccacgcgc gatcgagaac ggttcggtga gcacgccgag
     2161 gcggtcgtac ggaccgggcg cccacggatt gaagaacgca ggcagcacga agttgctcac
     2221 cgcgacgcgc atgccgctgg cgacgatgcg ataggcgtcg gcctgcaccg ggtcgcacac
     2281 ctcgcgcgac cacagcgtcc gcagtccgtt gaacgtgtat tcgttggcgg cggggtcgac
     2341 gagcatctcg aggacttcgt gggacgccgt cacggagatc gcatcctcgc cgtccaccca
     2401 cgtgctgccg cgtttgatcg agacgcccgc gaacaggtgc gcgtacggat cgccgtggcc
     2461 gtcgacgatg tgccagccgt agtcgtcggc ctggtgcgac gtgtcgaaga ggtggagctt
     2521 gtcgccgcgc ccgcccacgg tcactcgccg cggcttcacc ttccaagccg gcgcgaagtc
     2581 tcgttcgatc tgcgtggtga gcgcctcggc gatcgcgtcg agcaccccgg gttcaggacg
     2641 gtgcttccgc gtgtgatcga cgatcgcgat cgtgtccacg ccgggatcgt aagtcggtcc
     2701 ctgtgatgac ccgtgcctcg gccgcggcgt cacgcgcgtc cgttttgcga cgatgcgcag
     2761 ccttcggtaa cgtcctgctt tcccagtatt tgtccggaat agaccgtttc aggggaggca
     2821 cgatgaaggc actcgaccaa gacctcgaat cacgtcgctc gtttctccgg ttgggtggtg
     2881 tcgtcgccg
//
LOCUS       DASUGA010000478         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_300730, whole genome shotgun sequence.
ACCESSION   DASUGA010000478 DASUGA010000000
VERSION     DASUGA010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300730"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VFX21_09360"
     CDS             complement(<1..670)
                     /locus_tag="VFX21_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096210.1"
                     /translation="MSRDRVNDEIRYHAEVSYTLTTQRTALESALIPVTAYICVACVE
                     AYRRYPSMIEAITVAASPEELGRRGHELATQIDNVHLWAVPNFPLVGRKVLGGVGLLD
                     ADTDIASMSTIFDFWDRAARAYRFDDGTRQAWDADGVATPYRAFVDEIVAGCRPVRDD
                     EDRARISRLSALVTSYLFLLWFDTRSGYQDTGPYELAGGRSMLLRSFGRLGPSHFPWR
                     HDVAR"
     gene            complement(667..1029)
                     /locus_tag="VFX21_09365"
     CDS             complement(667..1029)
                     /locus_tag="VFX21_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEX5096211.1"
                     /translation="MTDTEACNIGTGTKVFEAPPVKGAWRRLSSPDDVLALMDTTAEG
                     VVACVADAGATFLAPIFDELTAVVCLAGTPMSHIGIVSREYQIPCVMGTVLNAQPEDG
                     AVVEVDCSGEQGVVRLMP"
     gene            1073..1657
                     /locus_tag="VFX21_09370"
     CDS             1073..1657
                     /locus_tag="VFX21_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5096212.1"
                     /translation="MLADECYVVLHEVRLRGLVEVSGELVDSPHVAALLAAGLVARAR
                     TSLRITAEGRETHAEWALLTAGSGEQQAAQRGYERFLPLNAEFLRLCHDWQVRPGEVP
                     NDHSDARYDWAVLDRLHALDERAAVVVRRIGRDVPRFEPYPGRLRAALRRVDEGEHEW
                     FTSPRIDSYHTVWMHVHEDLLLALGRDRADEPQR"
     gene            1654..>2289
                     /locus_tag="VFX21_09375"
     CDS             1654..>2289
                     /locus_tag="VFX21_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096213.1"
                     /translation="MTGTLSDDILTNAVGEFIERLRPIVMLWPEAKGADPAAVSRGLL
                     EEARRLSAGFLCSDGRLGDRELIAFRRSFGALDPQIANGPLSTLRANDSVKRDATFIE
                     KPSALFTQLLEQDRKQWTSNAWTYYEAALGIGHAMCALADIPTSDSLAALDRYRTMLL
                     GQLRTQNIVRPEADGAAAQNPSTLGGLPGEPPVAAPTLDALLEELDDLVGLV"
BASE COUNT          359 a          785 c          809 g          336 t
ORIGIN      
        1 cgcgggcgac gtcgtgtctc cacgggaaat gcgacggccc gaggcggccg aaggagcgca
       61 acaacatcga ccgcccaccg gccaactcgt aggggccggt gtcctggtag cccgagcgcg
      121 tgtcgaacca cagcaggaac aagtacgacg tcacgagcgc gctgaggcgc gagatacgcg
      181 cgcggtcttc gtcgtcgcgt acaggccggc aaccggcaac gatctcgtcc acgaacgccc
      241 ggtacggcgt ggcgacaccg tcggcatccc atgcctggcg cgtgccgtcg tcgaagcggt
      301 aggcgcgcgc ggcgcggtcc cagaagtcga agatcgtcga catcgacgcg atgtcggtgt
      361 cggcgtcgag cagaccgacg ccgccgagca ccttccggcc gacaaggggg aagttcggta
      421 cggcccacag gtgcacgttg tcgatctgcg tcgcgagctc gtgaccccgc cgaccgagct
      481 cttcgggtga cgcggccacg gtgatcgctt cgatcatgga cggatagcgg cggtacgcct
      541 cgacgcacgc gacacagatg tacgcggtca cggggatcag cgcggactcg agcgcggtgc
      601 gctgcgtcgt caacgtgtac gagacctccg cgtgataacg gatctcgtcg ttgacgcggt
      661 cgcgactcac ggcatgaggc ggacgacgcc ctgctcgccg ctgcagtcga cttcgacgac
      721 ggcgccgtct tcgggctgcg cgttcagcac cgtgcccatc acgcacggga tctggtactc
      781 gcggctgacg atgccgatgt gcgacatcgg cgtgcccgcg aggcacacga ccgcggtcag
      841 ctcgtcgaag atcggcgcga ggaacgtcgc gcccgcgtcg gcgacgcacg cgacgacgcc
      901 ttcggccgtc gtgtccatca gcgcgagcac gtcgtccggg gacgacaacc ggcgccacgc
      961 gcccttcacc ggcggcgcct cgaacacctt cgtccccgtc ccgatgttgc acgcctcggt
     1021 atccgtcatc ggctgcggaa tctaacggaa gggcctcatt aggatccggg acgtgttggc
     1081 ggacgagtgc tacgtggtgc ttcacgaggt gcggctgcgc gggctcgtcg aggtcagcgg
     1141 cgagttggtg gacagcccac acgtcgccgc gttgctcgcg gcgggcctgg tcgcgcgggc
     1201 gcgcacgtcg ctgcgcatca ccgcggaggg acgcgagacc cacgccgaat gggcattgtt
     1261 gacagcaggc tccggcgagc agcaggcggc gcagcggggc tacgagcgct tcctcccgtt
     1321 gaacgccgag ttcctgcgcc tctgccacga ttggcaggtg cggccgggag aggtccccaa
     1381 cgaccactcg gatgcccggt acgactgggc ggtgctcgac cggctccacg cgctcgacga
     1441 gcgggcggcc gtcgtcgtgc gccgcatcgg ccgggacgtg ccccgcttcg agccgtaccc
     1501 ggggcgactg cgggcggcgc tgcgccgggt cgacgagggc gagcacgagt ggttcacgtc
     1561 gccgcggatc gactcgtacc acacggtctg gatgcacgtg cacgaggatc tgctcctcgc
     1621 actgggccgc gatcgtgccg acgagccgca acgatgaccg gcaccctctc cgacgacatc
     1681 ctgaccaacg cggtcgggga gttcatcgag cggctgcggc cgatcgtgat gctgtggccg
     1741 gaggcgaagg gcgcggaccc ggcagcggtg tcgcgcgggc tgctcgaaga ggcgcgccgg
     1801 ctgtccgccg ggttcctgtg ctccgacggt cgcctcggag accgtgagct gatcgcgttc
     1861 cgtcgttcgt tcggcgcgct cgatccgcag atcgcgaacg gtcccttgag cacgttgcgc
     1921 gccaacgaca gcgtcaagcg cgacgcgacg ttcatcgaga agccgtccgc gctgttcacg
     1981 caactgctcg aacaagaccg gaagcagtgg acctcgaacg cgtggacgta ctacgaagcc
     2041 gcgctcggca tcggccacgc gatgtgcgcg ctcgccgaca taccgacgag tgactcgctc
     2101 gcggcgctcg atcgttatcg cacgatgctg ctcgggcagt tgcgcacaca gaacatcgtg
     2161 cgccccgagg ccgatggcgc ggccgcgcag aacccgtcga cgctcggcgg gctgccgggc
     2221 gaaccgccgg ttgccgcgcc gacgctcgac gcgctgctcg aggagctcga cgacctcgtc
     2281 gggctcgtc
//
LOCUS       DASUGA010000479         5163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1950738, whole genome shotgun sequence.
ACCESSION   DASUGA010000479 DASUGA010000000
VERSION     DASUGA010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950738"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1686
                     /locus_tag="VFX21_09380"
     CDS             <1..1686
                     /locus_tag="VFX21_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX5096214.1"
                     /translation="IAGVVAALALGNEDAALRVVLLVESAVVGVVAFGGFVSRYQRVG
                     AAYRARMQWLGWGITVAGALALVVWVLHMLLEWPEIAPELAIGATLFIPLSIALASID
                     SLALRVDKLLTRTVEAGGIVLMVGAIYLVVVLGFGDSPSSSERRVLGLSMVAGAVAAL
                     CYGPARARLSEFANRRVYGERQAPDEPIQTFGARMSRAIPLEELLLQLAESCKKSMQL
                     VAAEVWTGTGGVLELAASVPYRDAERIRMSIDEVTVVARAHVSGNAWAQVWLPGILQH
                     HGGRLVRVAPLAHSGELLGVILCARAGDDTPFTDEEDRVLTELARQVGLALHNSRLDT
                     ALQASLDDLRVANEELRASRSRIVVASDESRRQIERNLHDGAQQHLVALAVKLGLAKQ
                     LLASDPSVVEPMLDELRADAQETLTQLRELAHGIYPPLLIDRGIGEALRAAANRAVLP
                     TQVRADVGRFAPEVEAAVYFCCLEAMQNAGKHAGENAHITITVTTEEGELRFVVADNG
                     AGFDATSDAVRGHGFVNMADRVGAIGGVIDVKSAPGEGTCIAGRMPAIECEPE"
     gene            complement(1687..2058)
                     /locus_tag="VFX21_09385"
     CDS             complement(1687..2058)
                     /locus_tag="VFX21_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5096215.1"
                     /translation="MTVSVLIVDDQIPFRAVARTVVGLTDGFAVAGEAESGEDAVAKA
                     AAEPPDLVLMDINLPGINGIEATRRIRAANPTVSVILLSTYSESDLPADAREVGALAY
                     VHKEDFGPAIMRDLWDTKQSS"
     gene            complement(2150..2818)
                     /locus_tag="VFX21_09390"
     CDS             complement(2150..2818)
                     /locus_tag="VFX21_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5096216.1"
                     /translation="MALRVVIADDNLLVREGVVKLLDHQADVEVAATAGTYDELMAAV
                     ETCAPDVVVTDIRMPPTGTDEGIRAALEIREQHPQIGVVVLSQYADPAYALALLERGS
                     EARAYLLKERVSDPEQLTHAIAEVARGGSVIDPRVVESLVRARAARPASPVERLTPRE
                     REVLECMAQGRNNAGIAQALHLSERAVEKHINSLFSKLDLTEEPDVHRRVKAVLLLLA
                     EQGG"
     gene            2899..3198
                     /gene="rplU"
                     /locus_tag="VFX21_09395"
     CDS             2899..3198
                     /gene="rplU"
                     /locus_tag="VFX21_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX5096217.1"
                     /translation="MYAVIQTGGKQYRVSEGDRLDVERLAGDEVSLRPVMVVDGDTVL
                     ATPDQLSGASVTARIVGEAKGPKIKGFTYKNKSNQRKSWGHRQKYSTIEITGITR"
     gene            3205..3459
                     /gene="rpmA"
                     /locus_tag="VFX21_09400"
     CDS             3205..3459
                     /gene="rpmA"
                     /locus_tag="VFX21_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX5096218.1"
                     /translation="MSKKKGAASSRNGRDSNAQRLGVKRFSGETVTAGTIIIRQRGTK
                     WHAGFNVGRGCDDTLFAKSDGVVKFGSRRGRKLVDIVPSE"
     gene            complement(3460..3843)
                     /locus_tag="VFX21_09405"
     CDS             complement(3460..3843)
                     /locus_tag="VFX21_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX5096219.1"
                     /translation="MTRHGVSVWGSRELSVKGRKSGEWRTVPVNLLTLDGTRYLVAPR
                     GHTQWVRNLRAANGEGRLRVGKRVEEFRATEITDDAKPPILRAYLKRWKAEVGVFFGG
                     VSAKSTDEELRRISPDHPVFRLEAD"
     gene            3909..4535
                     /locus_tag="VFX21_09410"
     CDS             3909..4535
                     /locus_tag="VFX21_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096220.1"
                     /translation="MTMTGDDLRAATAWATNLLVPLAGRDWTVPCGDTEYTCRRTLNH
                     LADCMLFYAGALATRATRWAPDIRHGAPDETSVPQLLRAFEGAATILAVVTDEADASV
                     RAHHPAGMSDPEGFAAMGCDEVLVHAYEIATSVGAPPAEVPDELAARVVARLFPWAPN
                     GFGGLETLLWCNGRIALGPHKRQGPDWSWQCAPLAEWDGTVKKWRPRG"
     gene            complement(4542..>5163)
                     /locus_tag="VFX21_09415"
     CDS             complement(4542..>5163)
                     /locus_tag="VFX21_09415"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5096221.1"
                     /translation="LEEQNFAAAPRLLDVDEGGCLVLSYIDGFGAPDLDVSRWTPEQI
                     EAAFALLRRYHDITAGSPLAGSAEVVCHNDFTPQNVVFDDGAPVGIIDWEWAAPGSRR
                     RDLGHAIWQWLNVSAWGPPIDDLAQLIHRALGAYGLAADASIPADIEAREVEWLNIAT
                     QAARAGHDTWDRSPAHWTGTAQWVAEELRWLRSHATVLADAINAYA"
BASE COUNT          791 a         1745 c         1797 g          830 t
ORIGIN      
        1 atcgcgggag tcgttgccgc actcgccctc ggcaacgagg atgccgcgct gcgggtggtc
       61 ctcctcgtcg aatccgcggt cgtcggtgtc gtcgcgttcg gtggcttcgt ctcgcgctac
      121 caacgcgtcg gcgccgcata ccgggcccgc atgcagtggt tgggctgggg catcacggtg
      181 gccggcgcgt tggcgctggt cgtgtgggtg ttgcacatgc tgctggagtg gccggagatc
      241 gcgcccgagc tcgcgatcgg cgcgacgttg ttcatcccgc tctccatcgc actcgcgagc
      301 atcgactcgc tggcgctgcg cgtcgacaag ttgttgacgc gaaccgtcga agcggggggc
      361 atcgtgctga tggtcggcgc gatctacctc gtggtcgtgc tcggcttcgg tgattcgccg
      421 tcgtcgtccg agcggcgcgt gctcggcttg tcgatggtgg ccggcgccgt cgccgcgttg
      481 tgttacgggc cggcgcgcgc ccgtctctcc gagttcgcga accggcgggt ctacggcgaa
      541 cgccaagcac ccgacgaacc gatccagacc ttcggtgccc gcatgtcgcg tgcgatcccg
      601 ctcgaagagc tgctgttgca gctggcggag tcgtgcaaga agagcatgca gctcgtcgcg
      661 gccgaagtgt ggacgggcac cggcggcgtg ctcgagctcg cggcctcggt gccgtaccgc
      721 gacgcggagc gcatccgcat gtcgatcgac gaagtgacgg ttgtcgcccg tgcccatgtc
      781 tcgggcaacg cgtgggcgca ggtatggctc cccggcatcc tccagcacca cggtggccgg
      841 ctcgtccgcg tcgcgccgct cgcccactcg ggggagttgc tcggcgtcat tctctgcgca
      901 cgggccggcg acgacacgcc gttcaccgac gaggaggacc gcgtgctcac cgagctcgcg
      961 cgccaggtcg ggctggccct gcacaactcg cggctcgaca ccgcgttgca ggcatcactg
     1021 gatgatctgc gggtggccaa cgaggagcta cgcgcatccc ggtcgcgcat cgtcgtcgcg
     1081 agcgacgagt cgcgccgcca gatcgaacgc aatctgcacg acggcgccca acagcacctc
     1141 gtcgcgctgg cggtgaagct gggtctggcg aagcagctgc tcgcatccga tccgtcggtc
     1201 gtcgaaccga tgctcgacga gctgcgcgcc gacgcgcagg agaccttgac gcagctgcgc
     1261 gagctcgcgc acggcatcta cccaccgctg ttgatcgacc gcgggatcgg cgaagcatta
     1321 cgggcggccg cgaaccgtgc ggtgttgccg acccaggtgc gcgcggacgt cggtcggttc
     1381 gcaccggagg tcgaggccgc cgtgtacttc tgctgcctcg aggcgatgca gaacgccgga
     1441 aagcacgcgg gcgagaacgc gcacatcacg atcacggtca caaccgagga gggcgagctg
     1501 cgcttcgtcg tcgccgacaa cggcgccggg ttcgacgcga cgagcgacgc ggtccgtggc
     1561 cacggcttcg tgaacatggc cgaccgcgtc ggcgcgatcg gcggtgtgat cgacgtcaag
     1621 agcgcgccag gcgaaggcac gtgtatcgca ggccgcatgc ccgccatcga atgcgagccg
     1681 gaatgattac gaggactgct tcgtgtccca gagatcgcgc atgatcgccg ggccgaagtc
     1741 ttccttgtgc acgtaggcca gcgctccgac ctcgcgtgcg tccgccggga gatccgactc
     1801 gctgtacgtc gacagcagga tcaccgacac cgtcggattg gccgcgcgga tgcggcgcgt
     1861 cgcctcgatc ccgttgatgc cggggaggtt gatgtccatc agcacgaggt ccgggggctc
     1921 ggccgcggcc ttcgccaccg cgtcctcacc ggattccgcc tcgcccgcca ccgcgaatcc
     1981 gtcggtcagc cccacaaccg tgcgcgccac cgcccggaag ggaatctgat cgtcgacgat
     2041 gaggaccgac acggtcatgg tgggccgctc cgtagcgttc atgtcactga catcatcgtc
     2101 agaagggggc gatccgccca gggtgatcgc ttgcgagccg tgaggggtgc tagcccccct
     2161 gctcggccag gagcaagagg acggccttga cccgccgatg gacgtcgggt tcctcggtca
     2221 ggtcgagctt cgagaacagc gagttgatgt gcttttcgac cgcgcgctcg gagaggtgga
     2281 gcgcctgcgc gatgccggcg ttgttgcggc cctgcgccat gcactcgagc acttcccgtt
     2341 cacgcggcgt gagccgctcc acgggcgacg cggggcgcgc ggcgcgggcg cgcaccagcg
     2401 actcgacgac ccgggggtcg atcacgctgc cgccgcgcgc gacctcggcg atcgcgtgcg
     2461 tcagctgctc ggggtcggag acgcgctctt tgagcaggta ggcccgcgct tcggagcccc
     2521 gttcgagcag ggccagggcg tacgcggggt ccgcgtactg gctcaagacg accacgccga
     2581 tctggggatg ctgctcgcgg atctccagcg cggcccggat cccctcgtcg gtcccggtcg
     2641 gtggcatccg gatgtcggtc acgaccacgt cgggcgcgca ggtctcgacc gcggccatca
     2701 gctcgtcgta ggtgccggcc gtggccgcca cctcgacgtc cgcctggtgg tcgaggagct
     2761 tcaccacgcc ctcccggacc agcaggttgt cgtccgcgat caccacccgc agcgccatca
     2821 gggccggagg ctagtgcccg ggccggtctc ggctaatgtt gtccggttcc agaggaagta
     2881 cgtgagaggc gcgcgcgcat gtatgcggtg atccaaaccg ggggcaagca gtaccgggtc
     2941 agcgagggag accgcctcga cgtcgagcga ctcgccggcg acgaagtctc gctgcggccg
     3001 gtcatggtcg tcgacggcga caccgtgctc gccacccccg accagctctc gggtgcgtcg
     3061 gtcaccgccc gtatcgtcgg cgaggccaag ggcccgaaga tcaagggctt cacgtacaag
     3121 aacaagtcga accagcgcaa gagctggggc caccggcaga agtactcgac catcgagatc
     3181 accgggatca cgaggtaacc agccatgtcg aagaagaagg gcgccgcgtc ctcccgcaac
     3241 ggtcgcgact cgaacgcgca gcgcctcggt gtgaagcgct tcagcggcga gacggtcacc
     3301 gccggcacga tcatcattcg tcagcgcggc accaagtggc acgccggttt caacgtcggc
     3361 cgcggctgcg acgacacgct gttcgcgaag agcgacggcg ttgtgaagtt cggctcgcgg
     3421 cgcggccgca agctcgtcga catcgtcccg tcggagtagt cagtccgcct cgaggcggaa
     3481 cacaggatgg tcggggctga tccggcgtag ctcttcgtcg gtcgacttcg cgctcacgcc
     3541 gccgaagaag acgccgactt ctgctttcca ccgcttcagg tacgcgcgca ggatcggcgg
     3601 cttcgcgtcg tcggtgatct cggtcgcgcg gaactcctcg acgcgcttgc ccacgcggag
     3661 tcttccttcg ccgtttgcgg cgcgcaggtt gcggacccat tgggtgtggc cgcgtggcgc
     3721 cacgagatag cgggtgccgt cgagcgtgag caggttgacg ggcacggtgc gccactcgcc
     3781 gctcttgcgg cccttgaccg agagctcgcg ggaaccccag acgctgacgc cgtggcgcgt
     3841 caagaatgcg atcgtcgggt tgaagacgtg ccgggtgaac cagcccggtt gctgatagtg
     3901 aggcggcgat gacgatgaca ggcgacgacc ttcgcgccgc gaccgcctgg gccacaaacc
     3961 tgctggtccc gctggccggc cgcgactgga ccgtgccttg cggcgacacc gagtacacct
     4021 gccgccgcac cctgaaccac ctggccgact gcatgctctt ctacgcgggc gcgttggcga
     4081 cacgcgcgac ccggtgggcg cccgacatcc gtcacggtgc gcccgacgag acgagcgttc
     4141 cgcaactcct gcgcgccttc gaaggcgcgg cgacaatcct cgcagtcgtg accgacgaag
     4201 ccgacgcgtc agtgcgcgcg catcaccccg cgggtatgtc cgaccccgag ggcttcgccg
     4261 cgatgggctg tgacgaagtg ctcgtgcacg cgtacgagat cgcgacatcg gtcggcgcgc
     4321 caccggccga ggtgcccgac gagctcgcgg cgcgtgttgt cgcgcgcttg tttccgtggg
     4381 cgcccaacgg cttcggcggt ctcgagacgt tgctctggtg caacggccgc atcgcgctcg
     4441 gcccccacaa acgccaagga cccgactggt cgtggcagtg cgcgccactg gcagagtggg
     4501 acggcacggt gaagaagtgg cgcccgcgcg gctgacgcag ctcaggcata cgcgttgatc
     4561 gcgtcagcaa ggacggtcgc gtgcgagcga agccacctca attcctcggc aacccactgt
     4621 gcggtaccgg tccagtgtgc aggtgagcgg tcccaggtgt cgtggccggc gcgtgccgct
     4681 tgcgttgcga tgttcaacca ttcgacctcc ctcgcctcga tgtccgcagg gatgctcgcg
     4741 tccgcggcga gaccgtacgc gccgagtgcg cggtggatca gctgcgcaag gtcgtcgatc
     4801 ggtggtcccc acgcgcttac gttgagccat tgccagatag cgtggccgag gtcgcgtcgc
     4861 cgcgaaccgg gcgcagccca ctcccagtca atgatcccaa ccggcgcgcc gtcgtcgaag
     4921 acgacattct gcggtgtgaa gtcgttgtga catacaacct ccgccgaacc ggcgagcggg
     4981 ctaccggcgg tgatgtcgtg atagcggcgc agcagtgcga acgcagcttc gatctgttca
     5041 ggggtccagc gcgaaacgtc gaggtcgggt gcgccgaagc cgtcgatata cgacaacaca
     5101 agacacccgc cttcgtcgac gtcgagcagt cgcggtgccg cggcaaagtt ctgttcctcc
     5161 agg
//
LOCUS       DASUGA010000480         2106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_761587, whole genome shotgun sequence.
ACCESSION   DASUGA010000480 DASUGA010000000
VERSION     DASUGA010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761587"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFX21_09420"
     CDS             <1..345
                     /locus_tag="VFX21_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096222.1"
                     /translation="EASTMMQSASLVTDTRDGKSAVFDSWDIDATGNSFAGDLVDRWP
                     GFVYNQITTSQLFVPCRPELGGMALADQLATGTHVEGALDGDRLTLTLVGQSFDTEVR
                     FRATLEAERVEG"
     gene            complement(368..1579)
                     /locus_tag="VFX21_09425"
     CDS             complement(368..1579)
                     /locus_tag="VFX21_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004021656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5096223.1"
                     /translation="MYEWPEELQMIRDAVRDFVDREIRPHREELEHGDMPPYDLLRKL
                     YKTFGLDAMQRDSFLRRIEAEKNGTTAERSGGGGGGGMTLLPIIELCKCSPGMVTAMG
                     VSVGLTSAAILSKGTIAQKERWALDLLTLDKVGAWAITEPNSGSDAFGSMQSTARRDG
                     DEYILNGSKTFITNGPYADTIVFICKLDEGNPPEERTVLQFVLDKGMPGLEQSKPLRK
                     MGLHSSPTGMLFLDDVRVGRDRLLGESEEAFKGSGGREASKATFVMERSGVAAMALGI
                     IERCMDLCVAYAKERVQFGRPIGEFQLIQLKLAKMEVARLNVENLVFRSLESGQAGKP
                     MTLSQASAMKLYAAGAATEVALEAVQLFGGNGYMAEYEVEQLARDAKVLQIYAGTDEI
                     QVGQIARELLK"
     gene            1863..>2106
                     /locus_tag="VFX21_09430"
     CDS             1863..>2106
                     /locus_tag="VFX21_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096224.1"
                     /translation="MDAGSNSVERALPIDATLLSAVLLRLRRDVADRQARWTLGTRGA
                     MEMDVHFVPASAAPGVLGPVWTTTARLWDPAHLALAT"
BASE COUNT          342 a          733 c          684 g          347 t
ORIGIN      
        1 gaggcctcga cgatgatgca atccgcttcg ctcgtgaccg acacgcgcga cggcaagagc
       61 gcagtgtttg actcgtggga catcgacgcg acgggcaact cgttcgcggg cgacctggtc
      121 gaccgctggc ccggcttcgt ctacaaccag atcacgacgt cgcagctgtt cgtgccgtgc
      181 cgccccgagc tcggcgggat ggcactggcc gaccagctcg ccacgggcac gcacgtcgaa
      241 ggcgcgctcg acggcgaccg actcacgctc acactcgtcg gtcagagctt cgacaccgaa
      301 gtgcggttcc gcgcgaccct cgaggccgag cgcgtcgagg gctgaggagc agaagtcccg
      361 aagaccccta cttgagcagc tcgcgtgcga tctggccgac ctggatctcg tcggtgcccg
      421 cgtagatctg gagcaccttg gcgtcgcggg ccaactgctc gacctcgtac tcggccatgt
      481 agccgttgcc gccgaagagc tgcaccgcct ccagcgcgac ctccgtcgcc gcgcccgcgg
      541 cgtacagctt catcgcggac gcctgcgaca gcgtcatcgg cttgccggct tggccggact
      601 ccagtgatcg gaacacgagg ttctcgacgt tgaggcgcgc gacttccatc ttcgccagct
      661 tcagctggat gagctggaac tcgccgatcg gccggccgaa ctgcacgcgc tccttcgcat
      721 aggccacgca caggtccatg caacgctcga tgatgccgag cgccatcgcg gcgacacccg
      781 atcgttccat tacgaaggtc gccttcgacg cctcgcgtcc acctgagccc ttgaacgctt
      841 cctcgctctc gcccagcaac cggtcgcgac cgacacgcac gtcgtcgagg aacaacatgc
      901 cggtcggtga cgagtgcaga cccatcttgc gcaacggctt cgattgctcg agcccgggca
      961 tgcccttgtc gagcacgaac tgcaacaccg tgcgttcttc gggcgggttg ccttcatcga
     1021 gcttgcagat aaagacgatg gtgtcggcgt agggaccgtt cgtgatgaac gtcttcgagc
     1081 cgttcaggat gtactcgtcg ccgtcgcggc gcgcggtcga ctgcatcgac ccgaacgcgt
     1141 cactgcccga gttcggctcc gtgatggccc acgcaccgac cttgtcgagc gtcagcaagt
     1201 cgagcgccca ccgctccttc tgggcgatgg tgcccttcga gaggatcgcg gccgacgtca
     1261 gaccgaccga cacacccatc gcggtgacca tgcccggcga gcacttgcac agctcgatga
     1321 tcggcaggag cgtcatgccg ccgcccccgc cgccgccgga ccgctccgcg gtcgtgccgt
     1381 tcttctcggc ctcgatgcgg cgcaggaacg agtcgcgctg catcgcatcg agcccgaagg
     1441 tcttgtagag cttgcgcagg aggtcgtacg gcggcatgtc accgtgctcc agctcctccc
     1501 ggtggggccg gatctcacgg tccacgaagt cgcggactgc atcccggatc atctggagtt
     1561 cttcgggcca ctcgtacatc gcaaacgctc cccgtcggcg gacccgggca tcgtaggagg
     1621 aagcctgcct cgcgcccgat gtggcccgtt ccgactgcgc cgctacgcgg agtctccaac
     1681 aaggcggcgc cggagcctgc ggaggcgctc gcggagcgga gcgcgggcca acagtccggg
     1741 ctgcctcacg cgagcacgtg cggagcgaca aggagaaatg cgaacccgct caatcgtcgg
     1801 aggtgagccg ccgatatttg tccggacgct tacggatgag ttgtcactgg gggcacacga
     1861 gaatggacgc cggcagcaac agcgtggaac gcgcgctgcc aatcgatgcg acgttgctga
     1921 gtgcggtgtt gctgcggctg cgacgtgatg tcgcggaccg gcaggcacgc tggacgctcg
     1981 gcacgcgcgg cgcgatggag atggacgtgc acttcgtgcc cgcctctgcc gcgcccggcg
     2041 tcctcggccc cgtgtggacg accaccgcgc gtctgtggga ccccgcgcac ctcgcgctgg
     2101 cgaccg
//
LOCUS       DASUGA010000481         2578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1075158, whole genome shotgun sequence.
ACCESSION   DASUGA010000481 DASUGA010000000
VERSION     DASUGA010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075158"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /gene="pgl"
                     /locus_tag="VFX21_09435"
     CDS             <1..595
                     /gene="pgl"
                     /locus_tag="VFX21_09435"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX5096225.1"
                     /translation="QELVRAAPRSVALSGGGTAGECYEEVARRPFDWSAVDIYFGDDR
                     FVPPDHEDSNEGLARRTLLDACPPHSIHGMYHPGTVEAAADHYDALMRAVLPLDLVHL
                     GLGPDGHTASLFPGSSALDEHERMVIAASSEHHPHPRVTFTFAAIEKCPLVLVTVAGA
                     EKRDAMTRIAAGEDLPGARIRAERVVWLVDSAAAPEH"
     gene            606..>2578
                     /gene="cofG"
                     /locus_tag="VFX21_09440"
     CDS             606..>2578
                     /gene="cofG"
                     /locus_tag="VFX21_09440"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007297322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HEX5096226.1"
                     /translation="MPDLVRRPLEDLMAEAAARRDARFGTRITFSPKVFVPLTMLCRD
                     RCGYCTFAKPPARLDAPYLTPELILDIATRGARLGCHEVLFTLGEAPEARYPAAAEWL
                     AAHGYASTVDYLVAMAQLVVDETGLLPHANAGALDQAALERLRAVSPSQGMMIETLAA
                     RLGEPGGPHHGAPDKTPERRLATLEAAGRANVPFTTGILVGIGETREERIEALEAIAA
                     SHARHGHVQEVIVQNFLPKLGTAMHKAAPCPPDEFLWTIAAARLVLPDDVHLQAPPNL
                     SDDLAPLLAAGIDDWGGVSPVTIDHVNPERPWPALDRLRDATEAEGKVLAPRLTIYPE
                     YVQRPAEWIDEQLRTTVLVRSDSEGLARDTEWSPGRDDMTPPILLPAPARATGAVAAV
                     LQGVANGEEVGVDEIVTLLGARGPEVAAVAEVADQLRREIVGDEVTFVRNRNINYTNV
                     CTFKCRFCAFSKGPLSLNLRGNPYLLELEEMQHRVVEAVECGATEVCLQGGIHPDFDG
                     DYYVEVAEAVKAVAPDIHVHGFTALEVTEGARRLEMPLDEYLRRLKDAGLSTLPGTAA
                     EILDDEVRAVICPDKVNTEEWLHAHRTAHGVGLRSNITIMHGTVERPVHVARHMVRTR
                     DLQKETGGFTEFVPLPFVHMATPIFIQGKARRGP"
BASE COUNT          402 a          966 c          856 g          354 t
ORIGIN      
        1 gcaggaactg gtgcgcgccg cgccgcgatc ggtcgcactc tcgggcggtg gcaccgcggg
       61 tgagtgctac gaggaggtcg cgcgccgacc gttcgactgg tccgcggtcg acatctactt
      121 cggcgacgac cgcttcgtgc cgcccgacca cgaggactcg aacgaaggcc tcgcgcggcg
      181 cacgctgctc gacgcctgtc cgccgcacag catccacggc atgtatcacc ccggcaccgt
      241 cgaagcggcg gccgaccact acgacgcgct gatgcgtgcc gtgctccccc tcgacctcgt
      301 gcacctgggg ctcgggcccg acggtcacac ggcgtcactg ttcccggggt cgtccgcgct
      361 cgacgaacac gagcgcatgg tgatcgccgc gtcgtcggag catcacccgc acccccgcgt
      421 caccttcacg ttcgcggcga tcgagaaatg cccgctcgtc ctcgtcacgg tcgcgggcgc
      481 ggagaaacgc gatgcgatga cgaggatcgc ggccggcgag gacctccccg gcgcccgcat
      541 ccgcgccgag cgtgtggtct ggctggtgga ctcggccgcc gcacccgagc actagtgtgc
      601 gcggcgtgcc cgacctcgtg cgcaggccgc tcgaagatct gatggccgag gccgcggcgc
      661 gccgcgacgc ccggttcggc acccggatca ccttctcgcc gaaggtcttc gtcccgctga
      721 cgatgttgtg tcgcgaccgt tgcggctact gcacgttcgc caagccgccg gcccgcctcg
      781 acgcgcccta cctcacgccc gagctgatcc tcgacatcgc aacgcgcggc gcgcgcctcg
      841 gctgccacga agtgctgttc acgctgggcg aagcgcccga agcgcgctac ccggccgccg
      901 ccgagtggct cgcggcgcac ggttacgcat ccacggtcga ctacctcgtc gcgatggcgc
      961 agctggtcgt cgacgagacc ggtctgctgc cccacgccaa cgccggcgcg ctcgaccaag
     1021 ccgcgctcga gcgcctgcgg gcggtcagcc cgtcgcaggg gatgatgatc gagacgctgg
     1081 cggcgcgcct cggcgagccc ggcgggccgc accacggcgc gcccgacaag acacccgagc
     1141 gtcgcctcgc aacgctcgag gccgcgggac gcgccaacgt cccgttcacg accggcatcc
     1201 tggtcggcat cggcgagacg cgcgaggaac gcatcgaggc actcgaagcg atcgcggcgt
     1261 cacacgcgcg ccacggtcac gtgcaagaag tgatcgtgca gaacttcctg ccgaagctcg
     1321 gcaccgcgat gcacaaggcc gcgccgtgcc cacccgacga gttcttgtgg acgatcgcgg
     1381 ccgctcgctt ggtcctgccc gacgacgtcc atctgcaggc accaccgaac ctgagcgacg
     1441 acctcgcgcc gctgctcgcg gccggtatcg acgactgggg tggcgtctcc cccgtcacca
     1501 tcgaccacgt gaaccccgaa cgcccgtggc ccgcgctcga ccgactgcgc gacgcgaccg
     1561 aggccgaggg gaaggtgctc gcgcctcgcc tcaccatcta tcccgagtac gtgcagcggc
     1621 ccgccgagtg gatcgacgaa cagctgcgca ccacggtgct cgtccgttcc gacagcgagg
     1681 gcctcgcgcg cgataccgaa tggtccccgg gccgcgacga catgacgccc ccgatcctgc
     1741 tcccggcgcc tgcccgcgcc accggcgcgg tggccgcagt actccaaggc gtcgcgaacg
     1801 gcgaggaggt cggcgtcgac gagatcgtca cgctgctcgg cgcgcgtggc cccgaggtcg
     1861 ccgcggtcgc cgaagtcgcc gaccaactgc ggcgcgagat cgtcggcgac gaagtcacct
     1921 tcgtccgcaa ccgcaacatc aactacacga acgtgtgcac gttcaagtgc cggttctgcg
     1981 cgttctcgaa gggtccgctc tcgctcaacc tgcgcggcaa cccgtacctg ctcgagctcg
     2041 aagagatgca gcaccgggtc gtcgaagcgg tcgagtgcgg cgcgaccgag gtgtgcctcc
     2101 aggggggcat ccaccccgac ttcgacggcg actactacgt cgaggtcgcc gaggcagtga
     2161 aggcggtcgc gcccgacatc cacgtgcacg gcttcaccgc gctggaagtc accgaaggtg
     2221 cgcgccgcct cgagatgccg ctcgacgaat acttgcgccg gctgaaggac gccgggctgt
     2281 cgacgctgcc cggcaccgcg gccgagatcc tcgacgacga agtgcgcgcc gtcatctgcc
     2341 ccgacaaggt gaacaccgag gagtggctcc acgcacaccg caccgcgcac ggtgtcgggc
     2401 tgcggtcgaa tatcacgatc atgcacggca ccgtcgaacg gccggtgcac gtcgcccgcc
     2461 acatggtgcg cacccgggac ctgcagaagg agaccggcgg tttcaccgag ttcgtgccgt
     2521 tgccgttcgt gcacatggcg acgccgatct tcattcaggg caaagcgcgc cggggccc
//
LOCUS       DASUGA010000482         3571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1138907, whole genome shotgun sequence.
ACCESSION   DASUGA010000482 DASUGA010000000
VERSION     DASUGA010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138907"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="VFX21_09445"
     CDS             complement(<1..129)
                     /locus_tag="VFX21_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004003904.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HEX5096227.1"
                     /translation="MAGNRVAVVGIGQTAHKTARKDVSIAGLVREAAQEALDDAGMT"
     gene            complement(129..1184)
                     /locus_tag="VFX21_09450"
     CDS             complement(129..1184)
                     /locus_tag="VFX21_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018550730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HEX5096228.1"
                     /translation="MRDVGVVSFAYSAVAKDAQHSEVEMLSPVIREAVAASGIPRDQI
                     GFTCSGSFDYLQGGPFAFVSGLDAVGAWPPIRESHVEMDGAWALYEAWVAIQTGEVDS
                     ALVYGFGKASSAEDVYEIMAIQNDPYYVMPLHPTMIDLAALQASAYLDASGKDERALA
                     EVAARSQRDAQSNPQAVRKGDVSADDVLQQPKTHTPLRDADIFPITDGVAAMVIAADD
                     LARSVCTRPAWLRGIEHRVEPHALGVRDLTQSESTHLAGQAAGVHGGKIDIAELHAQF
                     SHEELILREALGLDGDTVINPSGGPLAANGLMSAGLVRIGEVARRITAGEADRGIAHA
                     SNGPCLQHNMVCVLEGE"
     gene            complement(1233..2219)
                     /locus_tag="VFX21_09455"
     CDS             complement(1233..2219)
                     /locus_tag="VFX21_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX5096229.1"
                     /translation="MTQVFTEHHVLEYPGGYTRSVGPVIGRFLTGLRAGRIEAVRLAD
                     GRVLVPPTEYDPTTSAPVAPEGDHWIQVGPCGIVQAYTWVANPKPGKHPLSRPFAFAL
                     IRPDGADTAILHVVDCGTEDAIAVGQRVTARWRAERTGHITDIESWYSLGDGEEPTPA
                     PVGAEGVDDEPVTGMTVPVRFDYEITAGEATTMYLKGLARGKIVGGRAEGATDVYAAS
                     RGTDPRTGEPTTVEVEVQDSGVVTTFCVVNVPGLSELAPEIPYVSAQVLLDGADNTFF
                     GLIRGAEVEDIHMGMRVKAKWADELKPDHRSILWWEPTGEPDADYDTYKDYL"
     gene            complement(2242..>3571)
                     /locus_tag="VFX21_09460"
     CDS             complement(2242..>3571)
                     /locus_tag="VFX21_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX5096230.1"
                     /translation="YYVPINYRLSPTEVAYILQDCEAKAFISHERFADVASAAAEEAG
                     IPAEHRLAHGAVPGFRSFGELVDAQPTTMPADRATGAAMHYTSGTTGKPKGVKRALAQ
                     IDPDTTAELFTFLLNLFGIQQNDGNVHLCTSPNYHTAVTTFAGNALHSKHTVVFMDKW
                     DPEEALRLIERYGCTHTHMVPTQFIRMLQLPDEVKQKYNVGSMKWAIHAAAPCPVDVK
                     RKMLEWWGPVIWEYYAATEGGGTIAPPDEWIKYPGTVGRAWPNAELKITDDEGNVMPT
                     GEPGTVWMRMGGGRNFEYKGDSEKTNKSHDAEGFFTVGDVGYFNEDGYLFLCDRKSDM
                     IIAGGVNIYPAEIESVIHEHPQVADVAVFGIPDPDMGEQIKAVVEPMEGVAADDALRT
                     SIMQHVQGKLGKYKWPRTVDFMDELPREPTGKLLKRKLRDPYWEGRDRAI"
BASE COUNT          508 a         1252 c         1176 g          635 t
ORIGIN      
        1 ggtcatcccc gcgtcgtcga gcgcttcctg tgcggcctcg cgtacgagcc cggcgatgga
       61 gacatccttg cgcgcggtct tgtgcgcggt ctgcccgatc ccgacgaccg caacccggtt
      121 gccggccact actccccctc caggacgcac accatgttgt gttgcaggca cggaccgttc
      181 gacgcgtgcg cgatgccgcg gtcggcctcg ccggcagtga tgcgccgggc gacctcgccg
      241 atgcgcacga gtccggcgct catcaatccg ttggcggcca gcggcccacc cgacgggttg
      301 atgacggtgt cgccgtcgag tccgagcgcc tcgcgcagga tcagctcctc gtggctgaac
      361 tgcgcgtgga gctcggcgat gtcgatcttc ccgccgtgca cgccggccgc ctgcccggcg
      421 agatgcgtcg actccgactg cgtgaggtca cgcacaccca acgcgtgcgg ttcgacgcgg
      481 tgctcgatgc cgcggagcca cgccggccgc gtgcataccg agcgggcgag gtcgtcggct
      541 gcgatcacca tggccgcgac accgtcggtg atcgggaaga tgtcggcgtc gcgtagcggc
      601 gtgtgcgtct tcggttgctg caagacatcg tcggcgctca cgtcgccctt gcgcaccgcc
      661 tgcgggttcg actgcgcatc gcgctgcgac cgcgcggcga cctccgccag cgcgcgctcg
      721 tccttgccgc tcgcgtcgag gtacgccgat gcctgcaacg cggcgaggtc gatcatcgtc
      781 ggatgcagtg gcatcacgta gtacgggtcg ttctggatcg ccatgatctc gtagacgtcc
      841 tcggccgacg acgccttgcc gaacccgtac acgagcgcgc tgtcgacctc acccgtctgg
      901 atcgcgaccc acgcttcgta cagcgcccat gcgccgtcca tctcgacgtg gctctcacga
      961 atcggcggcc acgcacccac ggcgtcgaga ccgctcacga acgcgaacgg cccgccctgc
     1021 aggtagtcga agctgcccga gcacgtgaag ccgatctggt cgcggggaat ccccgacgcc
     1081 gcgaccgcct cgcgaatcac cgggctcaac atctcgactt cgctgtgctg cgcgtccttg
     1141 gcgaccgcgg agtatgcgaa cgaaacaacg ccaacgtcac gcatgggtca ctcccgctcc
     1201 gccgtccacg gctgcgcttc gttctgtacg cattacaggt agtccttgta cgtgtcgtag
     1261 tccgcgtcgg gttcgcccgt cggctcccac cacaggatcg aacggtggtc gggcttcagc
     1321 tcgtcggccc acttcgcctt gacgcgcatg cccatgtgga tgtcttcgac ttccgcgccg
     1381 cggatgagcc cgaagaacgt gttgtcggcg ccgtcgagca gcacctgcgc ggacacgtac
     1441 gggatctcgg gtgcgagctc cgacaagccg ggcacgttca cgacacagaa cgtcgtcacg
     1501 acaccggagt cctggacctc gacttcgacg gtcgtcggtt cgccggtgcg cgggtcggtg
     1561 ccgcgcgacg cggcgtacac gtcggtcgcg ccttccgcgc ggccaccgac gatcttcccg
     1621 cgcgccaaac ccttgaggta catcgtcgtc gcctcgcccg cggtgatctc gtagtcgaag
     1681 cggaccggca ccgtcatacc ggtgacgggt tcgtcgtcga caccctcggc accgaccggc
     1741 gcgggcgtcg gctcctcgcc gtcaccgagc gagtaccacg actcgatgtc ggtgatgtgg
     1801 ccggtgcgct cggcgcgcca gcgggcggtg acgcgctgcc ccaccgcgat cgcgtcctcg
     1861 gtgccgcagt cgaccacgtg caagatcgcg gtgtcggcgc cgtcggggcg gatgagcgcg
     1921 aatgcgaacg gccgcgacaa cggatgcttg cccggcttcg ggttcgcgac ccacgtgtac
     1981 gcctgcacga tgccgcacgg acccacctgg atccagtgat cgccctcggg cgcgaccggc
     2041 gcgctcgtcg tcgggtcgta ctcggtcggc ggcacgagca cgcggccgtc ggccaggcgc
     2101 accgcttcga tgcggcccgc acgcagaccg gtcaggaacc gcccgatgac ggggccgacc
     2161 gagcgcgtgt agccgcccgg gtactcgagc acgtggtgct cggtgaaaac ctgtgtcact
     2221 gcagatcctt cttcgagaga actagatcgc gcggtcgcga ccttcccagt acgggtcgcg
     2281 caacttgcgc ttcagcaact tgccggtcgg ctcgcgcggc agctcgtcca tgaagtcgac
     2341 cgtgcgcggc cacttgtact tgccgagctt gccttgcacg tgctgcatga tcgaggtgcg
     2401 cagcgcgtcg tcggccgcga cgccttccat cggctcgacg acggccttga tctgctcgcc
     2461 catgtcggga tccgggatac cgaacaccgc gacgtcggcg acctgcgggt gctcgtggat
     2521 cacgctttcg atctcggccg ggtagatgtt cacaccgccg gcgatgatca tgtcgctctt
     2581 gcggtcacac aagaacaggt agccgtcctc gttgaagtac ccgacgtcgc cgacggtgaa
     2641 gaagccctcc gcgtcgtgcg acttgttcgt cttctcgctg tcgcccttgt actcgaagtt
     2701 gcggccgcca cccatccgca tccacacggt gcccggttca ccggtcggca tcacgttgcc
     2761 ctcgtcgtcg gtgatcttca gctccgcgtt cggccacgcg cgaccgacgg tgccggggta
     2821 cttgatccat tcgtcgggcg gtgcgatcgt gccgccgcct tcggttgcgg cgtagtactc
     2881 ccagatcacc gggccccacc actcgagcat cttgcgcttc acgtcgacgg ggcacggcgc
     2941 ggccgcgtgg atcgcccact tcatcgaccc gacgttgtac ttctgcttca cctcgtcggg
     3001 cagctgcagc atgcggatga actgcgtcgg caccatgtgc gtgtgcgtgc agccgtaccg
     3061 ctcgatgagg cgcaacgcct cttcggggtc ccacttgtcc atgaacacga ccgtgtgctt
     3121 cgagtgcagc gcgttgcccg cgaacgtcgt gacggcggtg tggtagttcg gcgacgtgca
     3181 gaggtggacg ttgccgtcgt tctgctggat gccgaacagg ttcaacaaga acgtgaacag
     3241 ctccgcggtc gtgtcggggt cgatctgcgc gagcgcgcgc ttcacgccct tcggtttgcc
     3301 ggtcgtgccc gacgtgtagt gcatggcggc accggtcgcg cgatccgcgg gcatggtggt
     3361 cggttgcgcg tcgacgagct ccccgaacga acggaacccg ggcaccgcgc cgtgcgcgag
     3421 gcggtgctcg gcggggatgc ccgcttcctc ggcggccgcg ctcgcgacgt cggcgaagcg
     3481 ctcgtggctg atgaacgcct tggcctcgca atcctggagg atgtaggcga cctccgtcgg
     3541 cgacagccgg tagttgatcg gcacgtagta c
//
LOCUS       DASUGA010000483         4374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_709225, whole genome shotgun sequence.
ACCESSION   DASUGA010000483 DASUGA010000000
VERSION     DASUGA010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709225"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VFX21_09465"
     CDS             complement(<1..542)
                     /locus_tag="VFX21_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096231.1"
                     /translation="MTFDASSEHSPLSGLDEYLVHNYPHPVRVMWTTDAQAYERIWFT
                     AQDDTGDLLVVCGLAFYPNLGTAEAFAIVNCRGQHTTVRAHRLLGDNRMDMRVGPIAY
                     EVVEPFKEWKLTLDENEYGTSYEIHWFDTKRAVFRNLGAGMIMDGKPFGGVAGYDGFG
                     EQTGWVKVGDERFELTRAHY"
     gene            complement(539..1585)
                     /locus_tag="VFX21_09470"
     CDS             complement(539..1585)
                     /locus_tag="VFX21_09470"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX5096232.1"
                     /translation="MATGAIRDTEMLRAGIEAWLADAFPERDGQRVDHLVRPAAGWST
                     ETVIAGVAWNGGHQRLVFRLPTVTPSFPSPALHEQGAVLDALATSAVPVPHAVAVVDD
                     PAWLGAPFLVMTFVDGRPVGDVPALDPWIMDAPVEQQRVVHEGFLRALASLHRADWQA
                     PGLDKHLRTGVAAEIEYWLGYVDWAAHGRPAPALVEAFEWCASTMPKGPKKKGASLLW
                     GDARLGNVMYERHARIVALVDWELATIGPAEMDLAWYLALDDLIASFMQDRRVPGFLP
                     RAEAIAFYENELGRPVSDLAWHELFALARSIAINDCQARLARAAGTEYPGIPGPENPI
                     LHILTDRIARFDGA"
     gene            1611..2768
                     /locus_tag="VFX21_09475"
     CDS             1611..2768
                     /locus_tag="VFX21_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5096233.1"
                     /translation="MNPEVLGTFTAPDYFDELARLRRESPVHQYAPNSWMVTRYDDVR
                     TMSRDPERFCSSKGVLISDPLRNGQQVEGSIIHMDPPQHGPWRKLTSRRFTPRSMSSM
                     EDVVRKVTREVLDELEPGVEVDFIDRVAAPIPVLVIAQLVGVDDADREDFRRWSDALI
                     ETPDHPERGAEDAAMLFSYLTRHAHERRANPTDDLASDIVHAEVNGEPVTTEQAVMYF
                     MTLLVAGNETTRHLISGGTAALAQHPEQRALLAREPERMTTAVEEFLRWVTPIQAFGR
                     TATIDLELGGEAIGAGDFLVMMYASANRDEAAFGPTADEFDVTRPVDNTHLAFGFGEH
                     LCLGAALARMEGRVVFEEFLKRFPNYELTAEPEIAPSTLVRGATKMPFVPR"
     gene            2776..>4374
                     /locus_tag="VFX21_09480"
     CDS             2776..>4374
                     /locus_tag="VFX21_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008743993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5096234.1"
                     /translation="MTRRSIADLVAARADDHHVGLLFEDQSWTWAEVVHECAVRAAVL
                     QAAGGDGPFHVGVLLENVPEYVFLLGGAALAGATVVGINPTRRGVELAADIRHTDCRI
                     IVTEAVHEPLLEGLDVGVTPERIFTIETRAWAKQLSEHGDAKIPEPLPGPEALFVLIF
                     TSGSTGRPKAVKGTQGRFATAGDAMPFSHDDVLYSAMPLFHGNALSASYVPAWQHGAA
                     IALRRKFSASEFMADVRRYNASYFNTVGRALSYVLATPPSPDDRNHRVKFALAPESSP
                     ADAAAFFERFGIYLVGGYGSSEGAIIIRPVRDAKPGALGKPADGDVAIVDADGNECAI
                     AELDADGKLLNADAAIGEIVRRDPAVVFEGYYNNPDATAERNRNGWYWSGDLGYRDAD
                     GIFYFAGRTADWIRVDGENFAAAPIERILGRHPDVAAVAVYAVPDPVTSDQVMAALEM
                     RTGVAFDTGTFEAFLEAQADLGTKWAPRFVRVVDAIPVTGADKIDKKPLRAAAWNASD
                     VWWRPARAEHYTPFDKDARAQLEAA"
BASE COUNT          729 a         1490 c         1461 g          694 t
ORIGIN      
        1 ttgtagtgcg cgcgcgtcag ctcgaagcgt tcgtcgccga ccttgaccca tccggtctgt
       61 tcgccgaagc cgtcgtagcc ggcgacgccg ccgaacggct tgccgtccat gatcatcccc
      121 gcgccgagat tgcggaacac ggcgcgcttg gtgtcgaacc aatgaatctc gtagctggtg
      181 ccgtactcgt tctcgtcgag cgtcagcttc cattccttga acggctcgac cacctcgtat
      241 gcaatgggtc cgacgcgcat gtccatgcgg ttgtcgccga gcaagcggtg cgcccgcacc
      301 gtcgtgtgtt gcccgcggca gttgacgatg gcgaacgcct cggcggtgcc gaggttcggg
      361 tagaacgcga ggccgcacac cacgagcagg tcgccggtgt cgtcctgggc ggtgaaccag
      421 atccgttcgt acgcctgggc gtcggtggtc cacatcacac gtacgggatg cggatagttg
      481 tggacgaggt attcgtcgag accgctgagc ggcgagtgtt ccgaactcgc gtcgaatgtc
      541 atgcgccgtc gaacctcgcg atgcggtcgg tcaggatgtg caggatcggg ttctcggggc
      601 cgggaatgcc ggggtattcg gtaccggccg cgcgcgccaa gcgagcttgg cagtcgttga
      661 tcgcgatcga gcgcgccaac gcgaacagct cgtgccaggc caagtcgctg acgggacggc
      721 cgagttcgtt ctcgtagaac gcgattgctt cggcacgcgg caagaagccg ggcacgcgcc
      781 gatcttgcat aaaactcgcg atgagatcgt cgagcgcgag gtaccacgcg aggtccatct
      841 cggccggacc gatcgtcgcg agttcccagt cgacgagcgc gacgatgcgc gcgtgccgct
      901 cgtacatcac gttgcccagg cgcgcgtcgc cccacaacaa cgacgcgcct ttcttcttcg
      961 gaccctttgg catcgtcgac gcgcaccact cgaacgcttc gacgagcgcg ggcgcgggcc
     1021 ggccgtgcgc ggcccagtcg acgtagccga gccagtactc gatctcggcc gcgacgccgg
     1081 tacgaaggtg cttgtcgaga ccgggtgcct gccagtcggc gcgatgaagc gaggccagcg
     1141 cgcgcaagaa cccctcgtgc acgacgcgct gctgctcgac cggcgcgtcc atgatccacg
     1201 gatcgagcgc ggggacgtcg ccgaccggac ggccgtcgac gaaggtcatg acgaggaacg
     1261 gcgcgccgag ccacgcggga tcgtcgacca ccgccaccgc gtgcggcact ggcaccgccg
     1321 aggtcgcgag cgcgtccagc acggcgccct gttcgtgcaa ggcgggtgat gggaacgagg
     1381 gggtcacggt cggcagccgg aacacgagcc gctgatgccc gccgttccac gccacgcccg
     1441 cgatcaccgt ttcggtcgac cagccggcgg ccgggcgcac gagatgatcg acgcgttggc
     1501 catcgcgttc cgggaacgca tcggccagcc aggcctcgat tccggcgcgg agcatttcgg
     1561 tgtcacgaat cgctcccgtc gccatgggtc cgcatgatac gaaaggcggc atgaatccgg
     1621 aggtcctcgg tacgttcacc gcacctgact acttcgacga gctggcgcgg ctgcgccgcg
     1681 agtcaccggt ccatcagtac gcgccgaact catggatggt gacgcgctac gacgacgtcc
     1741 gcacgatgag ccgtgatcct gagcggttct gctcgagcaa gggcgtactg atcagcgatc
     1801 cgctgcgcaa tgggcagcag gtcgagggct ccatcattca catggacccg ccccagcacg
     1861 gaccgtggcg caagctcacg agccgccgct tcacgcctcg ttccatgagc tcgatggaag
     1921 acgtcgtgcg caaggtgacg cgcgaagtgc tcgacgaact ggagcccggc gtcgaggtcg
     1981 acttcatcga ccgcgtggcg gcgccgattc cggtgctcgt catcgcgcag ctcgtcggcg
     2041 tcgacgacgc ggatcgcgag gacttccggc gctggtcgga cgccctgatc gagacgcccg
     2101 accatcccga gcgcggcgcc gaagacgcgg cgatgttgtt ctcgtacctc acacgccacg
     2161 cgcacgaacg gcgcgcgaat ccgaccgacg acctggcctc cgacatcgtg cacgccgaag
     2221 tgaacggcga accggtcacg accgagcagg cggtcatgta cttcatgacg ttgctcgtgg
     2281 ccggcaacga gacgactcgg catctcattt ccggtggaac ggccgcgctc gcacagcatc
     2341 cggaacaacg tgcgctcctc gcgcgtgaac ccgaacgcat gacgaccgcg gtcgaggagt
     2401 tcctgcgctg ggtgacacca atccaagcgt tcggccgcac cgccaccatc gatctcgagc
     2461 tcggcggtga ggcgatcggc gcaggcgact tcctcgtgat gatgtacgcg tcggcgaacc
     2521 gcgacgaggc ggcgttcggc ccgacggccg acgagttcga tgtgacccgt cccgtcgaca
     2581 acacgcacct tgcgttcggg ttcggcgagc acctgtgcct cggagccgcg ctcgcacgca
     2641 tggaaggacg cgtcgtcttc gaggagttcc tcaagcggtt ccccaactac gagctgaccg
     2701 ccgaaccgga gatcgcaccg tcgacgctcg tgcggggcgc gacgaagatg ccgttcgttc
     2761 cccggtgagg tcgacgtgac ccggcgctcg atcgccgatc tcgtcgccgc gcgcgccgac
     2821 gaccatcacg tcgggctgct gttcgaagac cagtcctgga cgtgggccga agtcgtgcac
     2881 gaatgcgcgg tgcgggctgc cgtgttgcaa gcagcgggcg gtgacgggcc gttccacgtc
     2941 ggcgtgctgt tggagaacgt gcccgagtac gtgttcctcc tcggcggcgc ggcgctcgcc
     3001 ggggccacgg tcgtcgggat caatccgacc cgccgcggcg ttgagttagc ggccgacatc
     3061 cgccacaccg actgccgcat catcgtcacc gaagcggtgc atgaaccgct gctcgagggt
     3121 ctcgacgtcg gtgtcacgcc ggaacggatc ttcacgatcg agacgcgggc gtgggcgaaa
     3181 cagctcagcg aacacggcga cgccaagatc ccggaaccgc tgccgggtcc ggaagccctg
     3241 ttcgtcttga tctttacgtc gggctcgacc ggccggccca aggccgtgaa gggaacgcag
     3301 ggccggttcg cgactgcggg cgacgccatg ccgttctcgc acgatgacgt gctctacagc
     3361 gcgatgccgc ttttccacgg caacgcactg tcggcgagct acgtgcctgc gtggcaacac
     3421 ggcgcggcga tcgcgttgcg tcgcaagttt tcggcgagcg agttcatggc cgacgtccgc
     3481 cggtacaacg cgagctactt caacaccgtc ggccgcgcgc tctcgtacgt gctcgcgaca
     3541 ccgccgtcgc ccgacgatcg caaccaccgc gtgaagttcg cgctggctcc ggagtcgtca
     3601 cccgccgacg cggccgcgtt cttcgaacgc ttcggcatct atctcgtcgg cggctatggc
     3661 tcgagcgaag gtgccatcat catccggccg gtgcgcgacg ccaagccggg cgcgctcggc
     3721 aagccggccg acggcgacgt cgcgatcgtc gacgccgacg gcaacgagtg cgcgatcgca
     3781 gaactcgacg cggacggcaa gctcctgaac gccgacgccg ccatcggcga aatcgtgcgg
     3841 cgcgatcccg ccgtcgtgtt cgagggctac tacaacaatc ccgacgccac cgccgagcgc
     3901 aaccggaacg gttggtactg gtcgggcgac ctcggttacc gcgacgccga cggcatcttc
     3961 tacttcgcgg gccggaccgc cgactggatt cgggtcgacg gcgaaaactt cgcggccgcg
     4021 ccgatcgaac gcatcctcgg ccggcatccc gatgttgcgg cagtcgcggt gtacgcggtc
     4081 cccgaccccg tcacctccga ccaggtaatg gctgcgctcg aaatgcgaac tggagtcgcg
     4141 ttcgacaccg gcacgttcga agcgtttctc gaagcccagg ccgatctcgg caccaagtgg
     4201 gcgccgcgct tcgtgcgcgt ggtcgacgcg attccggtga cgggtgccga caagatcgac
     4261 aagaagccac tgcgcgccgc cgcctggaac gcgtcagacg tgtggtggcg ccccgctcgc
     4321 gccgagcact acacgccctt cgacaaggac gctcgcgcgc agctcgaggc cgca
//
LOCUS       DASUGA010000484         5276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1548194, whole genome shotgun sequence.
ACCESSION   DASUGA010000484 DASUGA010000000
VERSION     DASUGA010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548194"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VFX21_09485"
     CDS             <1..214
                     /locus_tag="VFX21_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5096235.1"
                     /translation="MLALVRDRAAQHVQCSLAQTREWLEALGRTALAGVTRPGADDVA
                     DLMTTTGNVSHIRPAAPLRETPQHWD"
     gene            complement(223..903)
                     /locus_tag="VFX21_09490"
     CDS             complement(223..903)
                     /locus_tag="VFX21_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096236.1"
                     /translation="MLPIDVGFFSFTEITDPGEHHSYNEWHMLDHMPEQFPLPGVAWG
                     QRWVSTPACRSARSVSAPPLDAIHYVTCYFMTEPLDETLRDFYALGRELARAGRFHQH
                     RASHLSGPFSRDAIAAAPRVLVRPEAVPFRPHLGVYVVVAPAPVPVDALVECAGVAGA
                     WSFTAAGERFAAHPWQPGPRAVTVAYLDDAPLTVAPKIDALVRVHDGVEFAGPFETIT
                     PWHWDWFD"
     gene            complement(915..1325)
                     /locus_tag="VFX21_09495"
     CDS             complement(915..1325)
                     /locus_tag="VFX21_09495"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX5096237.1"
                     /translation="MSDPNAGIQAKIPLEASFIGTLQLEIDPSEEVGRLTGRMPVVDG
                     VCQPMGIVHGGVYASIAETIASMGTAHGVITNGGIPLGQANNTNFLRPVTEGTIHATG
                     TAFHRGRTSWVWDVEMRDDQGRLCATSRVTIAVR"
     gene            complement(1330..2517)
                     /locus_tag="VFX21_09500"
     CDS             complement(1330..2517)
                     /locus_tag="VFX21_09500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5096238.1"
                     /translation="MPTTTTMVTRPAPLQPDDDQFVTLAAKVGEIAAATAQAHDRDNT
                     FVREAYDAMRETGYLTLAVPTELGGLGATLRQAVYAQHELARHDGAAALASAMHTYLT
                     FVQVFRRRNGAPDAEGVLRRIADEGLVIATSGGSDWLWPNTIAVEENHTLRVSGRKMF
                     CSQAPAANMLTTSAVLGEPGEGSEVVMFSVPMNAPGVTIVETWDTLGMRGTSSHDVVL
                     DNVEVPVERIVARRPWGQFNPALLAAGIHIAPIIGAVYHGIANAARDEAVTRIAKATR
                     GPRPAASVERVQRQVGLMDARLRTSWWALMGALAELGDSYGADAETANTVMLAKRDVV
                     LAAREVTDLAMDAVGGASFFRSAPLERYARDVRAGTFHPFTPENTLTYAGRVALGEET
                     MTE"
     gene            2598..3263
                     /locus_tag="VFX21_09505"
     CDS             2598..3263
                     /locus_tag="VFX21_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5096239.1"
                     /translation="MKPYAQFCPVAKAAEVVGDRWSLLIVRDMIGGTTHFNDLVRGLP
                     GISRAILAKRLRQLEAAGLVVHGADGWALTEAGRDLEEIVFGLAAWGAKYAFGDPADD
                     ELDADLLMWWMHGRVAPDHRPDRMHVIQFVYRDHDHPYWLVIEPTDVSVCLTDPGFSV
                     DAVVRTDLRTMYNVWLGRRELLGAVRAGDIELDGPPAVVRKLPDWLQLSPVAPIVRDA
                     ART"
     gene            3273..3734
                     /locus_tag="VFX21_09510"
     CDS             3273..3734
                     /locus_tag="VFX21_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011042235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX5096240.1"
                     /translation="MPTLLVVSHSRSGGTQALTDAVVAGATADDIEGVAVRSLRAFDA
                     TADDVRAADAIALGTPENFGYMSGALKDFFERIYYVLLEETRGLPYALYVKASTDGDG
                     AVRSVERITTGLKWRAVLPPVVVVGDLQDAHLEAAHELGLTLAAGLEAGVF"
     gene            complement(3739..4677)
                     /locus_tag="VFX21_09515"
     CDS             complement(3739..4677)
                     /locus_tag="VFX21_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5096241.1"
                     /translation="MEHRSIGSLSVSVIGIGCNNFGGRIDEAATNIVVDTALESGINF
                     FDTADVYGGTKSEEFLGRALGSRRDTALIATKFGMPLDEERKGAAPAYIARAVEDSLC
                     RLGTDRIDLYQLHAPDPATPIEETLGALDELVRAGKVREIGCSNFSAEQIEEAEQCSR
                     DGRIARFVSVQNEYSLLRRGPERWGVLDACEKHGLGFLPYFPLSSGVLSGKYRRDQPP
                     PSGTRLAGMPDDRRTQLLSDKQMDKVEALEQWAAHHGHSLLELAFAWLLSRPVVSSVI
                     AGATKAEQVRTNAEAASWSLTHEDLDEIEAVLAAVG"
     gene            complement(4690..>5276)
                     /locus_tag="VFX21_09520"
     CDS             complement(4690..>5276)
                     /locus_tag="VFX21_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020556471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX5096242.1"
                     /translation="GSGATNDRPTFTSDGKTSVLDPETLDELPPGVQGLFARRGHIPI
                     GYWKDDAKTAATFRTDRNGVRWVLPGDFAVKDDEGRIVLFGRGSGCINTGGEKVFPEE
                     VEAAIRAHPAVLDAVVVGVPDDRFGERVTALVSVREGFDEPSLEDIQAHCRERIAGYK
                     VPRAVLLGEAPRTNVGKPDYATAKQIAREKTQSA"
BASE COUNT          799 a         1849 c         1787 g          841 t
ORIGIN      
        1 catgcttgcg ctcgtccgcg accgcgcggc gcaacacgtg cagtgctcac tcgcacagac
       61 ccgcgagtgg ctcgaagcgc tcggtcgcac ggcgctcgcc ggcgtcacgc gacctggcgc
      121 tgacgacgtc gccgacctga tgacgacgac cggcaacgtg tcacacatcc gtcccgcggc
      181 gccgctgcgc gaaacacccc agcactggga ctgacggagc agctagtcga accagtccca
      241 gtgccagggt gtgatcgtct cgaacggccc cgcgaactcg acgccgtcgt ggacgcgcac
      301 gagcgcgtcg atcttcggcg cgaccgtcaa cggcgcgtcg tcgaggtacg cgacggtgac
      361 cgcgcgcggt cccggctgcc acggatgcgc ggcgaagcgc tcgcccgcgg cggtgaacga
      421 ccacgcaccg gcgacacccg cacactcgac gagcgcatcg accggcaccg gcgccggtgc
      481 gaccacgaca tagacgccga gatggggacg gaacggtacg gcttcgggac gtacgagcac
      541 gcgcggcgca gccgcgatcg catcccgact gaacgggccc gacaggtgcg acgcacgatg
      601 ctgatggaag cgacctgcgc gtgcgagctc gcgccccagc gcgtagaagt cgcgcagcgt
      661 ctcgtcgagc ggctcggtca tgaagtaaca ggtgacgtag tggatcgcgt cgaggggcgg
      721 cgcgctcacc gaccgcgccg accggcacgc cggcgtcgac acccaccgct ggccccacgc
      781 gacaccgggc agcgggaact gctcgggcat gtggtcgagc atgtgccact cgttgtacga
      841 gtgatgctcg ccggggtcgg tgatctcggt gaacgagaag aacccgacgt cgatcggcag
      901 catgcgacga gtacctaacg caccgcgatc gtgacgcgcg aggtcgcgca caaacgaccc
      961 tggtcgtcgc gcatctcgac gtcccacacc cagctcgtgc ggccccggtg gaacgcggtg
     1021 ccggttgcat ggatcgtgcc ttcggtgacc ggccgcagga agttcgtgtt gttggcctga
     1081 ccgagcggga taccgccgtt ggtgatcacg ccgtgcgcgg tgcccatcga cgcgatcgtc
     1141 tccgcgatcg acgcgtacac gccgccgtgc acgatgccca tcggctggca cacaccgtcg
     1201 acgaccggca tgcgcccggt caggcgcccg acctcttccg acgggtcgat ctcgagctgc
     1261 aacgtcccga tgaagctcgc ttcgagcggg atcttcgcct ggataccggc gttcgggtcg
     1321 gacacaccct cactctgtca tggtctcctc accaagcgcg actcggcccg cgtacgtcag
     1381 cgtgttctcg ggggtgaacg ggtggaacgt gccggcgcgc acgtcgcgcg cgtagcgttc
     1441 gagcggcgcg ctgcggaaga aggacgcacc gccgacggcg tccatagcca ggtcggtgac
     1501 ctcgcgggcg gcgagcacga cgtcgcgctt ggccagcatg accgtgttcg cggtctcggc
     1561 atcggcgccg tacgaatcgc cgagctccgc gagtgcgccc atcaacgccc accagctcgt
     1621 gcgcaggcga gcgtccatca gcccgacctg ccgttgcacg cgttcgaccg acgcggccgg
     1681 cctcggtccg cgtgtcgcct tggcgatgcg cgtgacggcc tcgtcgcggg ccgcgttcgc
     1741 gatcccgtgg tacaccgcgc cgatgatcgg ggcgatgtgg atgccggctg ccaacaatgc
     1801 cggattgaac tgtccccacg gccggcgcgc gacgatgcgc tcgacgggga cctcgacgtt
     1861 gtcgagcacg acatcgtggc tcgacgtgcc gcgcatgccg agcgtgtccc acgtctccac
     1921 gatcgtcacg ccgggcgcgt tcatcggcac cgagaacatc accacctccg aaccctcacc
     1981 gggctcaccg agcactgcac tcgtcgtcag catgttcgcg gcgggcgcct ggctgcagaa
     2041 catcttgcgg ccgctgactc gcaacgtgtg gttctcttcc acggcgatcg tgttgggcca
     2101 gagccagtcg gagccaccac tcgtcgcgat gaccaggccc tcgtcggcga tgcggcgcag
     2161 cacaccttcg gcgtcgggcg cgccgttgcg gcgccggaag acctgcacga acgtgaggta
     2221 cgtgtgcatc gcggacgcga gcgcggccgc accgtcgtgg cgggccagct cgtgctgcgc
     2281 gtagacggcc tggcgcagcg tggcgcccag cccacccagc tcggtcggca ctgcgagggt
     2341 gagatagccg gtctctcgca tcgcgtcgta cgcctcgcgc acgaacgtgt tgtcacggtc
     2401 gtgggcctgc gcagtcgccg ccgcgatctc gccgaccttc gcggcaaggg tcacgaactg
     2461 atcgtcgtcg ggctgcaacg gggccggtcg ggtcaccatc gtcgtcgtgg tgggcattgg
     2521 gggctcctcg gaattggttg cgtcttggtc gaccgtacgg attgactggc ccgggaggaa
     2581 gttcaaagaa tgaaccggtg aagccctacg cccagttctg cccggtcgcg aaggctgcgg
     2641 aagtggtcgg ggaccgctgg tccctgctga tcgtgcggga catgatcggg ggcaccacgc
     2701 acttcaacga cctggtccgg ggcctgccgg gcatctcacg cgcgatcctc gccaagcgcc
     2761 tgcgccagct ggaggcggcc gggctcgtcg tgcacggcgc cgacggctgg gccctcaccg
     2821 aggcgggccg cgatctcgaa gagatcgtgt tcgggctcgc ggcgtggggc gcgaagtacg
     2881 cattcggcga tcccgccgac gacgagctcg acgccgatct gttgatgtgg tggatgcacg
     2941 gccgggtggc gcccgaccac cggcccgacc gcatgcacgt catccagttc gtctaccgcg
     3001 accacgacca tccctattgg ctcgtcatcg agccgaccga cgtctcggtg tgtctcaccg
     3061 accccggttt ctccgtcgac gcggtcgtgc gcacggatct gcgcaccatg tacaacgtct
     3121 ggctcggccg ccgggaactg ctcggcgcgg tgcgcgcagg cgacatcgag ctcgacgggc
     3181 cgcccgcggt cgtccgcaag ctgccggact ggctccagct cagcccggtg gcgccgatcg
     3241 tgcgcgacgc ggcacgcacg taggggcact aggtgccgac gctcctcgtc gtttctcatt
     3301 cgcgttcggg tggcacccag gcgctgactg acgccgtggt cgcgggcgcg acggcggacg
     3361 acatcgaagg cgtcgcggtg cgttcgctgc gcgcgttcga cgccactgcg gacgacgtcc
     3421 gcgcagccga cgcgatcgcg ctcgggacgc ccgagaactt cggctacatg agtggcgcgt
     3481 tgaaggactt cttcgagcgc atctactacg tgctgctcga ggagacgcgc ggcctgccgt
     3541 acgcgttgta cgtgaaggcg agcaccgacg gcgacggtgc ggtgcgcagc gtggagcgca
     3601 tcacgaccgg gttgaagtgg cgagccgtgc tgccgccggt cgtcgtcgtc ggcgacttgc
     3661 aggacgcgca tctcgaagcc gcgcacgagc tcgggctcac cctggcggcc ggcctcgaag
     3721 ccggcgtgtt ctagcgcgtc aaccgacggc cgcgaggacg gcctcgatct cgtcgaggtc
     3781 ctcgtgggtc aacgaccacg atgccgcttc cgcgttcgtg cgcacctgct cggccttcgt
     3841 cgcgcccgcg atcacgctgg acacgacggg acgcgacagc aaccacgcga acgcgagctc
     3901 cagcaacgaa tgaccgtgat gcgcggccca ctgctcgagc gcttcgacct tgtccatctg
     3961 cttgtcggac aacaattgcg tgcggcgatc gtcgggcata cccgcgagcc gcgttcccga
     4021 cggcggcggc tggtcgcggc ggtacttccc gctcagcaca ccgctgctca gcgggaagta
     4081 cggcaggaag ccgaggccgt gcttctcaca cgcgtcgagc acaccccaac gttccgggcc
     4141 ccggcgcagc aggctgtact cgttctgcac gctcacgaac cgggcgatgc gcccgtcgcg
     4201 gctgcactgc tcggcttcct cgatctgctc cgccgagaag ttgctgcacc cgatctcgcg
     4261 caccttgccc gcccgcacca attcgtcgag cgcgccgagc gtctcctcga tcggtgttgc
     4321 gggatccggc gcgtgcagtt ggtagaggtc gatgcgatcg gtgccgagcc ggcacaggct
     4381 gtcttcgacc gcgcgtgcga tgtacgcggg cgccgcaccc ttgcgttcct cgtcgagcgg
     4441 cataccgaac ttcgtggcga tcagggccgt gtcgcggcgc gagcccaatg cacgacccag
     4501 gaactcttcg ctcttcgtac cgccgtagac gtcggcggtg tcgaagaagt tgatgcccga
     4561 ttcgagcgcg gtgtcgacga cgatgttcgt ggccgcttcg tcgatacgcc cgccgaagtt
     4621 gttgcagccg atcccgatca ccgagacgga caacgaaccg atgctgcggt gctccatcta
     4681 ccaactatgt cacgcagact gcgtcttttc ccgggcgatc tgtttggcgg ttgcatagtc
     4741 gggcttgccg acgttcgtgc gcggcgcctc tcccaagagc accgcacgcg gcaccttgta
     4801 gcccgcgatg cgttcccggc aatgggcttg gatgtcttcc agcgacggct cgtcgaaccc
     4861 ctcgcgcacg ctgaccagcg ccgtgacgcg ttcgccgaag cggtcgtcgg gcacgccgac
     4921 cacgaccgcg tcgagcaccg ccgggtgcgc acggatcgcg gcctcgacct cctcggggaa
     4981 gaccttctcg ccaccggtgt tgatgcagcc cgaaccgcgg ccgaacagca cgatgcggcc
     5041 ttcgtcgtcc ttcactgcga agtcaccggg cagcacccag cgcacaccgt tgcgatcggt
     5101 gcggaaggtc gccgccgtct tcgcatcgtc cttccagtac ccgatcggga tgtggccgcg
     5161 gcgcgcgaac aagccttgga cgcccggcgg cagctcgtcg agcgtctcgg gatcgagcac
     5221 cgacgtcttc ccgtcgctcg tgaacgtggg ccggtcgttc gtcgcgccgg agccga
//
LOCUS       DASUGA010000485         3177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_613518, whole genome shotgun sequence.
ACCESSION   DASUGA010000485 DASUGA010000000
VERSION     DASUGA010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613518"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VFX21_09525"
     CDS             complement(<1..366)
                     /locus_tag="VFX21_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5096243.1"
                     /translation="MTVAYEVRDRIAHVTIDNGKANAISPGVIDGLNDALTKAEDAGD
                     AEVGAIVITGTPGMLSGGFDLEVMRSGPAAAGKLVTDGGECFSRMYGAPVPIVVACTG
                     HAIAAGALLMMGADVRIGAR"
     gene            complement(363..1385)
                     /gene="ruvB"
                     /locus_tag="VFX21_09530"
     CDS             complement(363..1385)
                     /gene="ruvB"
                     /locus_tag="VFX21_09530"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX5096244.1"
                     /translation="MREELLSPAAADDIEIEEEVTLRPRRLDEFVGQPRLREHLEIML
                     TAARNRGQAVDHVLLAGPPGLGKTSMAGIIANEMGARITPTSGPALERVGDLAAILTN
                     LDDGDVLFIDEVHRLPRVVEEGLYPAMEDFRFDVVIGKGPSARTIRLDLPRFTLVGAT
                     TRTGLITGPLRDRFGFVARLDYYDADDLAAILRRTASILGVELEADGADEIARRARGT
                     PRIANRLLKRVRDYAEVRADGQVSADTARAALTLFEVDELGLDKVDRAILHALCSTFA
                     GRAVGLGTLAVSVGEAPETVEDVYEPFLLQCGLIERTPRGRIATPAAFGHAGLRPPQG
                     PPSLLE"
     gene            complement(1385..1975)
                     /gene="ruvA"
                     /locus_tag="VFX21_09535"
     CDS             complement(1385..1975)
                     /gene="ruvA"
                     /locus_tag="VFX21_09535"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX5096245.1"
                     /translation="MIGSLRGCVIERTLNGEVLLEVGGVGYRVNLPLGALSSCEPGTN
                     AFLFTHLHVREDAMILYGFPSRDERDTFEALIGATGVGPKLALAILSVHNPNALRRCL
                     ADDDLDALVLVPGVGKRTAQRLLVELKSRLEVPDLDLTAVPGTSTSNARAEVRDALVN
                     LGYSAEEVRDVVATLGDDGTVEDLLRDALRALAGGR"
     gene            complement(1972..2478)
                     /gene="ruvC"
                     /locus_tag="VFX21_09540"
     CDS             complement(1972..2478)
                     /gene="ruvC"
                     /locus_tag="VFX21_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX5096246.1"
                     /translation="MFDPPGPVLGIDPGLSRCGYGVVAREGGKLRALSAGVIRTPPSF
                     EVPARLGILDEELTDLILQVRPGVVVVERVLFQANVRTAMSVGQASGVALVIASRANI
                     PVVQYSPNEVKLAVTGDGRADKVAVQTMVMRLLALKEVPKPPDAADALALACCHLGRA
                     RMMQQVSR"
     gene            complement(2555..2920)
                     /locus_tag="VFX21_09545"
     CDS             complement(2555..2920)
                     /locus_tag="VFX21_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5096247.1"
                     /translation="MDIKETVATYGAAWNEPDEAKRAALLEASWADEGIYQDPTATAE
                     GRAALVAHIGGFREMFPGRSIDQASGVDITDGGLRFAWVMRNGNDVELEGMDFVELAP
                     DGRIKRIAGFFGPFPPLDA"
     gene            complement(2923..>3177)
                     /locus_tag="VFX21_09550"
     CDS             complement(2923..>3177)
                     /locus_tag="VFX21_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096248.1"
                     /translation="GGGDTSHMGGEYEQALESALKNTRTSAPAATATSGPRKIDSPEA
                     EPVDLFEMAGAPLTKRLIPIGIGALVLLILWRILRSNSKG"
BASE COUNT          503 a         1085 c         1085 g          504 t
ORIGIN      
        1 gcgcgcgcca atgcgcacgt cggcgcccat catgagcaac gcacccgcgg caatggcgtg
       61 gcccgtgcag gcgaccacga tcggcaccgg tgcgccgtac atgcggctga agcattcgcc
      121 gccatcggtg acgagcttgc cggccgcggc cggaccgctg cgcatgactt cgagatcgaa
      181 gccgcccgac aacatgccgg gcgtgccggt gatcacgatc gcgccgactt cggcgtcgcc
      241 ggcatcttcg gctttcgtca acgcgtcgtt cagcccgtcg atgacgccgg gcgagatcgc
      301 gttcgctttg ccgttgtcga tggtgacgtg cgcaatgcga tcgcgcactt cgtaagcaac
      361 cgtcattcca acagacttgg cggtccctgc ggcgggcgca aacccgcatg tccgaaggcc
      421 gccggcgtgg cgatccggcc ccggggcgtt cgttcgatca ggccgcattg gaggaggaag
      481 ggttcgtaga cgtcctcgac cgtttccggc gcctcgccga ccgagactgc gagcgtgccg
      541 agcccgaccg cgcgccccgc gaacgtgctg cagagcgcgt ggaggatggc ccggtcgacc
      601 ttgtcgaggc cgagctcgtc gacctcaaac aaggtgaggg cggcgcgcgc cgtgtcggca
      661 ctgacctggc cgtcggcccg tacctcggcg tagtcgcgca cccgcttcaa caagcggttc
      721 gcgattcggg gcgtgccgcg cgcccggcga gcaatctcgt cggcgccgtc cgcctcgagc
      781 tcgacaccga gaatgctcgc ggtgcggcgc aggatcgcgg ccagatcgtc ggcgtcgtag
      841 tagtcgagcc gggcgacgaa cccgaagcgg tcgcgcagcg gcccggtgat gagtccggtg
      901 cgcgtcgtgg cgccgaccag cgtgaagcgg ggcaagtcga ggcgaatcgt gcgcgcactc
      961 gggcccttgc cgatcacgac gtcgaaccgg aagtcctcca tcgcgggata caggccctcc
     1021 tcgacgacgc gcggcaagcg gtgcacttcg tcgatgaaca acacgtcgcc gtcgtcaagg
     1081 ttcgtgagga tggcggccag atcgccgacc cgttcgagcg cgggtccgct cgtgggcgtg
     1141 atgcgggcgc ccatctcgtt ggcgatgatg ccggccatgc tcgtcttgcc gagcccgggc
     1201 ggaccggcca gcagcacgtg atcgaccgcc tgcccgcggt tgcgcgcggc cgtgagcatg
     1261 atctcgaggt gctcgcgcag ccggggctgg ccgacgaact cgtcgagccg gcgcggccgc
     1321 agcgtgactt cttcttcaat ctcgatgtcg tcggcggccg ccgggctgag gagttcctct
     1381 ctcattaccg ccctcccgcg agcgcgcgca gggcgtcgcg cagcaaatct tcgacggtcc
     1441 cgtcgtcgcc gagcgtcgcg acgacatcgc gcacctcttc ggcggagtag ccgagattga
     1501 ccaacgcgtc acggacctcg gcgcgcgcat tggaggtgga cgtacccggc accgcagtca
     1561 gatcgagatc cggcacctcg aggcgcgact tgagctcgac gagcagacgc tgcgcggtgc
     1621 gcttcccgac gcccgggacg agcacgagcg cgtcgagatc gtcgtcggcg aggcagcgcc
     1681 ggagcgcgtt tggattgtgc accgacaaga tcgcgagcgc gagcttcggt ccgaccccgg
     1741 tcgcaccgat gagggcctcg aacgtgtcgc gttcgtcgcg cgacgggaag ccgtagagga
     1801 tcatggcgtc ctcgcgcacg tgcagatgcg tgaagaggaa cgcgttcgtg cccggttcgc
     1861 aactgctcag cgcgccaaga ggaaggttta cgcggtagcc gacgccgccg acttcgagca
     1921 acacctcgcc gttcagcgtc cgttcgatga cacagccccg caacgaaccg atcatcggct
     1981 cacctgctgc atcatgcgcg cccggccgag gtgacagcac gcgagggcga gcgcatcggc
     2041 cgcgtcgggc ggcttgggaa cttccttgag cgcgagcagc cgcatgacca tcgtttggac
     2101 cgcgaccttg tcggcacgcc cgtcgccggt gaccgcgagc ttgacctcgt tcggcgagta
     2161 ctggacaacc ggaatgttgg cccgggacgc gatgacgagg gcaacgccac tcgcctgccc
     2221 caccgacatt gcggtccgaa cgttcgcttg gaacaacacg cgttcgacca cgacgacgcc
     2281 cggccggacc tgcaggatca ggtcggtgag ctcttcgtcg aggatgccga ggcgcgccgg
     2341 cacttcgaac gacggcggcg tccggatcac gcccgccgac agcgcgcgca gcttgccgcc
     2401 ttcgcgcgcg acgacgccgt agccgcaacg cgacaacccc gggtcgatac cgagcaccgg
     2461 accaggcgga tcgaacacgg gttcgatact agaagcgatc aacgcgaaag cccgggatcc
     2521 ccggaaagca cctgttcagc ctgtcaaacg caggtcacgc gtcgaggggc gggaacggtc
     2581 cgaagaaacc tgcgatgcgc ttgatgcggc cgtcgggcgc gagttcgacg aagtccatgc
     2641 cttcgagctc gacgtcgttg ccgttgcgca tgacccacgc gaagcgcagc ccgccgtcgg
     2701 tgatgtcgac accgctcgct tggtcgatgc tgcgacccgg aaacatctcg cggaacccgc
     2761 cgatgtgtgc gaccagcgcg gctcgacctt cggcggttgc agtcgggtct tgatagatcc
     2821 cttcgtccgc ccacgacgct tcgagcaacg ccgcgcgctt cgcttcgtcg ggttcgttcc
     2881 acgccgcccc gtacgtcgcg acagtctctt tgatgtccat cttcagcctt tgctgttgct
     2941 gcgcaggatg cgccacagga tgaggagcac tagcgcaccg atcccgatgg ggatcaggcg
     3001 tttcgtgagg ggcgcgccgg ccatctcgaa caggtcgacg ggctcggcct cgggcgagtc
     3061 gatcttgcgg ggcccgctcg tcgcggtggc agcaggtgcc gacgtgcgcg tgttcttgag
     3121 cgcgctctcg agggcctgtt cgtactcgcc gcccatgtgc gaggtgtcgc cgccgcc
//
LOCUS       DASUGA010000486         2431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1130216, whole genome shotgun sequence.
ACCESSION   DASUGA010000486 DASUGA010000000
VERSION     DASUGA010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130216"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..1031
                     /locus_tag="VFX21_09555"
     CDS             270..1031
                     /locus_tag="VFX21_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096249.1"
                     /translation="MTSASREWITIPVPGKEKKQWQIDVTFLASSYRCIFGQGCQGVL
                     TEPAAELVQGCCSYGAHANDKKDQEKITKLAKKLTDEEWQFRAVGMKKGVWAKVGKDD
                     YRTRLVEDACVFLNRPGFAAGPGCALHVHAQNHGKHFVDTKPIVCWQLPIRTIDRDEE
                     DESITTVLTEFGRDGWGEGGEEFAWWCTEAKEAFTGAEPVYKSMEAELRATLGDDVYE
                     QVCKYMDTRVSSAVPPLPHPAEVPVTLGRTRIRRP"
     gene            1028..1777
                     /locus_tag="VFX21_09560"
     CDS             1028..1777
                     /locus_tag="VFX21_09560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEX5096250.1"
                     /translation="MSATATVADLAAEQAQLRAVLDGIGASDWLRPTPSWGWDVRDTI
                     AHLADTDEMAIDTMTGGPRALGLVRESSVSSEDTTYRGVLRGRRMTGAGVGAWWAEMS
                     ARELDTIAALDPEMRVPWGLGMKAPSFISARIMETWAHGLDVHAALGTEPVDSDRLAF
                     IAWLATRALPYAYTVAGREPPPGDLRVELTLPSGAPWIFGPEGAAGRITGPVGQYCRV
                     FTQRLRLADAPDLEATGAVAESALAVARAYL"
     gene            1788..2342
                     /locus_tag="VFX21_09565"
     CDS             1788..2342
                     /locus_tag="VFX21_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX5096251.1"
                     /translation="MPRYSFEDLTPNVHESAFVAPTATLVGDVIVEAGASIWYGAVIR
                     ADYAPVIIRRGANVQDNAVIHGPPGLTTDVGPGVTVGHNCVVHGAILEEECLIANGAI
                     VLDGATVGARALVAANSVVAAGASIPAGMLATGAPAIVKRPVEGSGGQMWVDLNPSAY
                     AELAQRHRHGIRYVDPHADDDDQS"
BASE COUNT          389 a          839 c          859 g          344 t
ORIGIN      
        1 cggccagccc ggtgccgctc ccgcgggcga cgaagggacg accgtgacgc ctcgcgacct
       61 gcaccgcggc cgcgacctgg gccgcggaat cgggccagca caccgcgagc gcattcccgg
      121 tcgcgacacc gccgtcgcgc gcgtacagcg ccatctcgag tggttcgttg ctggcgcgcg
      181 caccgaccgc ggcgcgcagg tcgttcagga gcgcatcaga ccgagcgatt gtcgcagcct
      241 accgaccatc ccggtatacc ctccgggcca tgacgtctgc ctcgcgcgag tggatcacca
      301 tcccggtgcc cggcaaggaa aagaagcagt ggcagatcga cgtgacgttc ctcgcgtcgt
      361 cgtaccgctg catcttcggg cagggctgcc agggtgtgtt gaccgaaccg gccgccgagc
      421 tggtccaggg ttgttgctcg tacggcgcgc acgcgaacga caagaaggac caagagaaga
      481 tcacgaagct cgccaagaag ctcacggacg aggagtggca gttccgcgcg gtcggcatga
      541 agaagggcgt ctgggccaag gtcggcaagg acgactaccg cacccgtttg gtcgaggacg
      601 cgtgtgtgtt cctgaaccgg cccggcttcg cggccggccc cggttgcgcg ttgcacgtgc
      661 acgcgcagaa ccacggcaag cacttcgtcg acacaaaacc gatcgtctgc tggcagttgc
      721 cgatccgcac gatcgaccgc gacgaagaag acgaatcgat cacgacggtg ctcaccgagt
      781 tcggtcgtga cggctggggt gaaggcggcg aagagttcgc gtggtggtgc acggaggcca
      841 aggaggcgtt caccggcgcc gagcccgtgt acaagtcgat ggaagccgaa ctgcgcgcaa
      901 cgctcggcga cgacgtctac gagcaagtgt gcaagtacat ggacacgcgc gtctcgagtg
      961 cggtgccgcc gctgccccac cccgccgagg tgccggtgac cctcggccgc acgcggatcc
     1021 ggaggccgtg agcgcgaccg cgacggtcgc cgacctcgcg gccgaacagg cccagctgcg
     1081 cgccgtcctc gacggcatcg gcgcgagcga ctggttgcgg cccacgccct cgtggggctg
     1141 ggacgtccgc gacacgatcg cccacctcgc cgacaccgac gagatggcga tcgacaccat
     1201 gacgggcggg ccgcgtgccc tcggactcgt tcgcgagtcg tcggtgtcga gcgaggacac
     1261 gacctaccgc ggtgtgctgc gcggccgccg catgacgggc gccggcgtcg gcgcgtggtg
     1321 ggccgagatg tcggcccgcg aactggacac gatcgcggcg ctcgatccgg agatgcgcgt
     1381 gccgtggggt ctcggcatga aggcgccgtc gttcatctcg gcgcgcatca tggagacatg
     1441 ggcgcacggg ctcgacgttc atgccgcgct cggtaccgag cccgtcgaca gcgaccgcct
     1501 cgcgttcatc gcgtggctgg cgacgcgcgc gctgccgtac gcgtacacgg tcgccgggcg
     1561 cgaaccgccg cccggtgacc tgcgcgtcga gctgacgctg ccgtcgggcg cgccgtggat
     1621 cttcggcccc gagggcgccg ccggccgcat caccggcccc gtcggccagt actgccgcgt
     1681 cttcacacag cggctgcgat tggccgacgc acccgatctc gaggcaaccg gcgccgtcgc
     1741 cgaatccgcg ctcgccgtcg cacgcgcgta tctctgaggg ggcactcatg ccgcggtatt
     1801 cgttcgagga cctcacgccg aacgtgcacg agtcggcgtt cgtcgcgccg accgcgacgc
     1861 tcgtcggtga cgtgatcgtc gaggcgggcg cgtcgatctg gtacggcgcg gtgatccgcg
     1921 ccgactacgc gccagtgatc attcgccgcg gtgcgaacgt gcaggacaac gcagtcatcc
     1981 acggaccacc tggtctcacg accgatgtcg gacctggggt gaccgtgggt cacaactgcg
     2041 tcgtgcacgg cgcgatcctc gaagaggagt gtctcatcgc gaacggtgcg atcgtgctcg
     2101 acggggcaac cgtcggtgcg cgtgcgctcg tcgccgcgaa ctcggtcgtc gcggcgggtg
     2161 cgagtatccc ggcgggaatg ctcgcgacgg gcgcgccggc gatcgtaaag cgtcccgtcg
     2221 agggcagcgg cggccagatg tgggtggacc tgaacccgag cgcgtacgcg gagctcgccc
     2281 aacgtcaccg ccacggcatt cgatacgtcg atccacacgc cgacgacgac gatcagtcgt
     2341 aaactcagga cccgcgctgt cagccgcgcg ccgacacgcc ggcaccgccg tcgacgacga
     2401 gggtcacctc gtgacgtcgg ggggccgcgt c
//
LOCUS       DASUGA010000487         3889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_748614, whole genome shotgun sequence.
ACCESSION   DASUGA010000487 DASUGA010000000
VERSION     DASUGA010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748614"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VFX21_09570"
     CDS             complement(<1..295)
                     /locus_tag="VFX21_09570"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014985987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5096252.1"
                     /translation="MHGFVATGFEPVLDAFVANFDERGEVGAALCIYKDGQPIVDLWG
                     GLADATTGRAWAEDTVVLVFSSTKGVTSVCANLCIERGLLDPDARVAQYWPEFA"
     gene            complement(299..1006)
                     /locus_tag="VFX21_09575"
     CDS             complement(299..1006)
                     /locus_tag="VFX21_09575"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5096253.1"
                     /translation="MVGEHDRLAPADAIPAAGDDCDLPVEQAHLRVPSACKKTDVGVT
                     VARPRGRPPSPRPCSRPCFREDEVAVDTSAVGARTGAWRVVLDRSVLGNFAGAVGDKS
                     RVYRDRDAAGDAGFTNIPAPPTFSFAAPFWSAYYADEQPDDPTAGKGNPMHTIMGELY
                     AKGALVLHGEQEFEYHRVPVVGDVLDGEQMITDIYEKDTDNANMTFVVMETTWRDAAN
                     GEPVITERFNLIARIKK"
     gene            920..1855
                     /locus_tag="VFX21_09580"
     CDS             920..1855
                     /locus_tag="VFX21_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013426620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5096254.1"
                     /translation="MGLLDGKVAIVTGSGNGVGRGEAIMLADHGAKVVVNDLGGSVTG
                     EGSDKRAADDVVDVIKKRGGEAVANYDSVADFEGAKNIIGTALDAFGRLDVLVNNAGV
                     LRDKMMFSMSPDDFDLVVKVHMYGTWNTMHHASVYWRNESKEGRQPRASIVNTVSSAG
                     LQGQASQINYGAAKAGIGAMTIIASLELKRYGVRANCIGPGGATRMVAQAMPNMEVKN
                     PEDYESFENMNPGNSAPGVVYLASDESLHVTGQVLRLVGNNLCVYRPWEMGEQFLATE
                     KDGSPKQWDPIDIGRILNRYHFNSENPGIDAQRRA"
     gene            1856..2698
                     /locus_tag="VFX21_09585"
     CDS             1856..2698
                     /locus_tag="VFX21_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5096255.1"
                     /translation="MPSVKIAQFETIDYAESDDGVATVTLNRPEVHNAFNSLMQRELH
                     ALWRALRRHDPVKCIVLTGAGDKAFCTGIDRMEQMGGEGDDTTDPDVVGSGTTPFMFN
                     DPGDNLGPKSCDLWKPVIAAVNGMACGGAFYMLGEVEFIIAAEHASFFDPHVTYGMCA
                     SFEPIHMSGIMPFPEIMRVSLLGNYERMSAQRAHQIGMVSEIVPGPELLGRAQELATI
                     IASQPRLAVEGTVRALWSTREMPQREAVRLGYAYVAMGTNQDSIAEGQELFASGKRVE
                     WKLR"
     gene            2695..3834
                     /locus_tag="VFX21_09590"
     CDS             2695..3834
                     /locus_tag="VFX21_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEX5096256.1"
                     /translation="MTVAIVGAALSDIGRVDVKNAFELHYQAASRAIADAGLTKDDID
                     GFGSCGTGLLAPIEVAEYCGVRPDWAESTGVGGASWEFMVEHAAAAIDAGHANVVVLV
                     YGSTTRADLKAKRRSANLSFGARGPVQFDAPFGHSLISKYAMATRRHMHEFGTTVDQL
                     AEIAVSARYNASLNPEAYYRDLITREDVHNSRMIADPLTKLHCCIRSDGGGAVVLTSA
                     DRAKDLPKKPVWVLGTGEATSHTTMSEWRDFTESPAIRSGRAAFARAGVKPADIDTCQ
                     LYDAFTSMVLLTLEGLGFCEKGEGGAFVDDGKLRVGGALPTNTDGGGLSHCHPGMRGM
                     FLLVEAVRQLRGEADGRQVENAKLACVNGTGGWFSAASTTILGVD"
BASE COUNT          672 a         1295 c         1324 g          598 t
ORIGIN      
        1 cggcgaactc gggccagtac tgcgcgaccc gcgcgtcggg atcgaggaga cctcgttcga
       61 tgcagaggtt ggcgcagacg gatgtgacgc ccttcgtcga ggagaagacg agtaccacgg
      121 tgtcctcggc ccatgcccgg cccgtcgttg cgtcggcgag cccgccccag aggtcgacaa
      181 tgggttgtcc gtccttgtag atgcagagcg cggcgccgac ctcgccgcgc tcgtcgaagt
      241 tcgcgacgaa cgcatcgagc accggctcga acccggttgc gacgaagccg tgcacgacct
      301 acttcttgat gcgcgcgatc aagttgaacc gttccgtgat gacgggctcg ccgtttgcgg
      361 cgtcgcgcca cgtcgtctcc atgacgacga acgtcatgtt cgcgttgtcg gtgtcctttt
      421 cgtagatgtc ggtgatcatc tgctcgccgt cgagcacgtc gccgacgacg ggtacgcggt
      481 gatattcgaa ctcctgctcg ccgtgcagta cgagcgcgcc cttcgcatac agctcgccca
      541 tgatcgtgtg catggggttg cccttgcccg cggtggggtc gtcgggttgc tcgtctgcgt
      601 agtacgcgct ccagaacggc gccgcgaacg agaacgtcgg gggcgcggga atgttcgtga
      661 agcccgcgtc gccggccgcg tcgcggtcgc gatacacgcg actcttgtcg ccgaccgcac
      721 ccgcgaagtt gccgagcacg gatcggtcga gcacgacacg ccatgctccc gttcttgcac
      781 cgacagcgct ggtgtcgacg gccacttcgt cctcccggaa acacggtcgt gagcagggtc
      841 gcgggcttgg cgggcggccg cgagggcgcg ctacggtgac gcccacgtca gttttcttgc
      901 acgcggaagg aacgcgcaaa tgggcttgct cgacgggaag gtcgcaatcg tcaccggcag
      961 cgggaatggc gtcggccggg gcgaggcgat catgctcgcc gaccacggcg ccaaggtcgt
     1021 cgtcaacgac ctcggcggtt cggtgacggg tgaaggctcc gacaaacggg ccgccgacga
     1081 cgtcgtcgac gtcatcaaga agcggggcgg cgaggcggtc gccaactacg acagcgtcgc
     1141 cgacttcgag ggcgccaaga acatcatcgg caccgcactc gacgcgttcg gccgtctcga
     1201 cgtgctcgtg aacaacgcgg gcgtgctgcg cgacaagatg atgttctcga tgtcgcccga
     1261 tgacttcgac ctcgtggtca aggtccacat gtacggcacc tggaacacga tgcaccacgc
     1321 gtcggtgtac tggcgcaacg agtcgaagga aggccgccag ccgcgcgcct cgatcgtcaa
     1381 cacggtctca agcgcgggtc tgcagggcca ggccagccag atcaactacg gcgcggccaa
     1441 ggccggcatc ggcgcgatga cgatcatcgc gtcgctcgag ttgaagcgct acggcgtgcg
     1501 cgccaactgc atcggccccg gtggcgcgac gcgcatggtc gcgcaggcaa tgccgaacat
     1561 ggaggtcaag aaccccgaag actacgagtc gttcgagaac atgaaccccg gcaactccgc
     1621 gccgggcgtc gtgtacctcg cctccgacga gtcgctgcat gtcacgggcc aggtgctgcg
     1681 cctcgtgggc aacaacctct gcgtctaccg gccgtgggag atgggtgagc agttcctcgc
     1741 gaccgagaag gacggctcac cgaagcagtg ggacccgatc gacatcggcc gcatcctcaa
     1801 ccgctaccac ttcaacagcg agaaccccgg gatcgacgcc cagcggaggg catgagtgcc
     1861 cagcgtcaag atcgcgcagt tcgagacgat cgactacgcc gagtccgatg acggtgtcgc
     1921 gaccgtcacg ctgaaccggc ccgaagtcca caacgcgttc aactccctga tgcaacgtga
     1981 gctgcacgcg ctgtggcgcg cgttgcggcg tcacgacccg gtgaaatgca tcgtgctcac
     2041 cggcgcgggc gacaaggcgt tctgcaccgg catcgaccgc atggagcaga tgggcggcga
     2101 gggcgacgac accaccgacc ccgacgtcgt cggctccggg accacgccgt tcatgttcaa
     2161 cgacccgggc gacaacctcg gcccgaagtc gtgcgacctc tggaagccgg tgatcgcggc
     2221 ggtgaacggc atggcgtgcg gcggcgcgtt ctacatgctc ggcgaggtgg agttcatcat
     2281 cgcggccgag cacgcgtcgt tcttcgaccc gcacgtcacc tacgggatgt gtgcgtcgtt
     2341 cgaaccgatc cacatgtcgg ggatcatgcc gttccccgag atcatgcggg tctcgctgct
     2401 cggcaactac gagcgcatgt ccgcgcagcg ggcgcaccag atcggcatgg tctcggagat
     2461 cgtgcccggg ccggagctgt tggggcgcgc acaagagctc gcgacgatca tcgcgtcgca
     2521 gccgcgcctc gcggtcgagg gcaccgtgcg cgccctctgg tcgacgcgtg agatgccgca
     2581 acgcgaagca gtacggctcg ggtacgcgta cgtcgcgatg ggcacgaacc aggactccat
     2641 cgccgaaggc caggagttgt tcgcgtcggg caagcgcgtg gagtggaaac tgcgttgacc
     2701 gtcgccatcg tcggtgcggc gctctccgac atcggacgcg tcgacgtcaa gaacgccttc
     2761 gagctccact accaagcggc gtcgcgcgcg atcgcggacg cgggactcac caaggacgac
     2821 atcgacggct tcggctcctg cggcaccgga ctgctcgcgc cgatcgaagt ggccgagtac
     2881 tgcggcgtac ggcccgactg ggccgagagc accggcgtcg gtggggcgtc gtgggagttc
     2941 atggtcgagc acgcggccgc cgcgatcgac gcggggcatg cgaacgtcgt cgtgctcgtc
     3001 tacgggtcga cgacgcgcgc cgacctcaag gcgaagcgcc gcagcgccaa cctctcgttc
     3061 ggggcgcgcg ggccggtgca gttcgacgcg cccttcggac attcgctgat ctcgaagtac
     3121 gcgatggcga cgcgccgcca catgcacgag ttcggcacga cggtcgacca gctcgcggag
     3181 atcgcggtgt cggcgcgcta caacgcgtcg ctcaatcccg aggcgtacta ccgcgacctg
     3241 atcacgcgcg aagacgtcca caactcgcgg atgatcgccg acccgctcac gaagctgcac
     3301 tgctgcatcc gcagcgacgg cggcggcgca gtggtgctga cgagcgcgga ccgggccaag
     3361 gacctcccga agaagccggt gtgggtattg ggcacgggcg aagcgacgtc gcacacgacg
     3421 atgagcgaat ggcgcgactt cacggagtcg cccgcgatcc gcagcggccg cgccgcgttc
     3481 gcgcgagccg gcgtgaagcc ggccgacatc gacacgtgcc agctgtacga cgcgttcacg
     3541 tcgatggtgc tgctcaccct cgaaggcctc ggcttctgcg agaagggcga aggcggcgcg
     3601 ttcgtcgacg acggcaagct gcgggtcggc ggcgccctgc ccaccaacac cgatggcggc
     3661 ggcctctcgc actgccaccc cggcatgcgc ggcatgttcc tgctggtcga agccgtgcgc
     3721 cagctgcgcg gcgaagccga cggccgccag gtcgagaacg cgaagctcgc gtgcgtcaac
     3781 ggcaccggcg gctggttctc cgccgccagc accaccatcc tcggcgtcga ctgaagaaat
     3841 tctcgggcgt cttacaaggg cggattccgc ccttgtaaga cgcccgaag
//
LOCUS       DASUGA010000488         2524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_987889, whole genome shotgun sequence.
ACCESSION   DASUGA010000488 DASUGA010000000
VERSION     DASUGA010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987889"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VFX21_09595"
     CDS             complement(<1..797)
                     /locus_tag="VFX21_09595"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX5096257.1"
                     /translation="MPARMAQRMHDDEIDSDPELVRALLAAQQPQWAALPIERVESTG
                     TDNAMYRVGDDVVVRMPLRPGRAAETLAKEHEWLPVLAPQLPLRIPVPLASGEPTDFY
                     PWPWSVYPWLAGEDATTAPLDLTQAAVDLAQFIRALGAIATTGAPEPSAANFGRGVPL
                     ATRDDYTRAAIAASERLIDTEAVTAAWEAALRAPAWGGAPAWIHGDLASGNLLVADGR
                     LSAVIDWGGAAIGDPACDMHIAWEMFDGPSREIFRAETGVDDATWARG"
     gene            859..>2524
                     /locus_tag="VFX21_09600"
     CDS             859..>2524
                     /locus_tag="VFX21_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096258.1"
                     /translation="MAVDERKTDVDLLASVAPRHWAPIVFGVVPAGANRRRPSDIVRV
                     FLAAFVVVIAGIGANHVAVLEKRVFELIADLPDWIHEFSEFWFKAATYGTLTALLLAL
                     LLARRFRLVLVLAVAGALGWLAAVGLRSLVDVDDERHAAGLVLDGTVPKHPVALLAIT
                     TTMLFVAGPYLLRPARRLVFFALGVAAFGAIFGLVGLPDDVVASIALGWGVAAALHLA
                     IGTPAATPTLAQVSNELLALGVTVSDLAIDPHQQWGESRFVATAPDGGAISVEVIGRD
                     ATDARLFAKVGRSIWYKDSGPALALTRSQQLEHRAYLLLLADRAGVPVSEVVIAGVAG
                     KRETALLVLREAEGTPLDQVDAAEITDAVLDNAWQNLARLHRERVAHGSMGAANVRLR
                     ADGTTAFVDFSRASSTAPPERLLLDRVELLVTTAAIVGPEHAFGAAQRALGPDGLADM
                     LPMLEPQALSAGARHDVANLKPLLKDLREQGALLTGEEAVTLTELRRVSPTDILMAAG
                     AILGVYLLIGELADVDFQEVFDTAEWGWVFVAFCLSFLPPFSSSISM"
BASE COUNT          332 a          926 c          847 g          419 t
ORIGIN      
        1 ccgcgcgccc aggtcgcgtc gtcgacacct gtttcggcgc ggaagatctc acgcgacggc
       61 ccgtcgaaca tctcccacgc aatatgcatg tcgcacgcgg gatcaccgat ggccgccccg
      121 ccccagtcga tgactgccga aagacgcccg tccgcgacga gcaagttccc cgacgcgagg
      181 tcgccgtgga tccacgcggg cgcgccgccc catgccggcg cgcgcaatgc ggcttcccac
      241 gcggcggtca ccgcttcggt gtcgatcagc cgctccgacg ccgcaatcgc ggcgcgtgtg
      301 tagtcgtcac gggtcgcgag cggcacacca cggccgaagt tcgcggcgct cggctcgggc
      361 gcgcccgttg tcgcgatcgc tcccaacgca cgaatgaact gcgccagatc gaccgcggcc
      421 tgtgtgaggt cgagcggcgc ggtcgtggcg tcctcaccgg ccagccacgg gtagaccgac
      481 cacggccagg gatagaagtc ggtcggttcg cccgacgcga gcggcaccgg gatccgcaac
      541 ggcagctgcg gcgcgagcac gggcaaccat tcgtgctcct tcgcgagtgt ctccgcggcg
      601 cgtcccggtc gcagcggcat gcgcaccacg acgtcgtcgc cgacgcggta catcgcattg
      661 tcggtgccgg tcgactcgac gcgctcgatc gggagcgcgg cccactgcgg ctgttgcgcg
      721 gcgagtaatg cgcgcaccag ctccggatcg ctgtcgatct cgtcgtcgtg catgcgttgc
      781 gccattcggg cgggcatccc actccgatca ccccgctgtg tccagcgaat tacgggcccg
      841 atcggccaga ctggcaccgt ggccgtcgac gaacgcaaga ctgacgtcga cctcctcgcg
      901 tccgtcgcgc cgcgccactg ggcgccgatc gtgttcggcg tggtgcccgc gggcgccaat
      961 cgccgccgcc cgagcgacat cgtgcgcgtc ttcctcgcgg cattcgtcgt cgtcatcgcg
     1021 ggcatcggcg cgaatcacgt tgccgtgctc gagaagcgcg tcttcgagct gatcgcggac
     1081 ctacccgatt ggatccacga gttctcggag ttctggttca aggccgcgac gtacggcacg
     1141 ctcaccgcgc tgctgctcgc cctgttgctc gcgcgccggt tccgcctggt gctcgtcctg
     1201 gccgttgccg gtgccctcgg ctggttggcc gccgtcggtc tgcggtcgct cgtcgacgtc
     1261 gacgacgaac ggcacgccgc cggactcgtc ctcgacggca ccgtccccaa gcacccggtc
     1321 gccttgctcg ccatcaccac gacgatgttg ttcgtggccg gaccgtacct gttgcggccc
     1381 gcgcgccggc tcgtgttctt cgcgctcggc gtcgcggcgt tcggcgcgat cttcggtctc
     1441 gtcggcctcc ccgacgacgt cgtcgcgagc atcgcgttgg gctggggtgt cgcggccgcg
     1501 ctgcatctcg cgatcggaac gcccgcggct acgccgaccc ttgcgcaggt ttcgaacgag
     1561 ctcctcgcgc tcggcgtcac ggtctccgat ctcgcgatcg acccgcacca gcagtggggt
     1621 gagtcccggt tcgtcgcgac cgcgcccgac ggcggtgcga tctccgtcga ggtgatcgga
     1681 cgcgacgcga ccgacgcgcg cctgttcgcc aaggtcgggc ggtcgatctg gtacaaggac
     1741 tccggtccgg cactcgcgtt gacgcgcagc cagcagctcg agcaccgtgc ctatctgtta
     1801 ctgctcgccg accgggccgg tgtgccggtg agcgaggtcg tgatcgccgg cgtcgcgggg
     1861 aaacgcgaga ccgcactgct ggtgctgcgc gaggccgagg gcacaccgct cgaccaggtc
     1921 gatgcggccg agatcaccga cgccgtgctc gacaacgcgt ggcagaacct cgcgcgactg
     1981 caccgcgagc gcgtcgccca cgggtcgatg ggcgccgcca acgtgcgcct gcgcgccgac
     2041 ggaacgacgg cgttcgtcga cttctcgcgt gcgtcgtcca cggcgccgcc cgaacgcttg
     2101 ctgctcgacc gtgtcgaact gctcgtcacg accgcggcga tcgtcgggcc cgagcacgcg
     2161 ttcggcgccg cgcaacgggc actcggcccc gacggcctcg ccgacatgct ccccatgctc
     2221 gaaccgcaag cgctgtcggc cggcgcccgt cacgacgtcg ccaacttgaa gccgctcctg
     2281 aaggatctgc gcgagcaagg cgcgctgctc accggcgagg aagccgtcac gctgaccgag
     2341 ctgcggcggg tgtcgcccac cgacatcttg atggcggccg gtgcgatcct cggtgtctat
     2401 ctgctcatcg gtgagttggc cgacgtcgac ttccaggagg tcttcgacac cgccgagtgg
     2461 ggttgggtct tcgtcgcgtt ctgcctctcg ttcctgccgc cgttcagcag ctcgatctcg
     2521 atga
//
LOCUS       DASUGA010000489         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1093941, whole genome shotgun sequence.
ACCESSION   DASUGA010000489 DASUGA010000000
VERSION     DASUGA010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093941"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2131
                     /locus_tag="VFX21_09605"
     CDS             <1..>2131
                     /locus_tag="VFX21_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEX5096259.1"
                     /translation="TRLSASVLARLHYVLHVDPTDRHEVDLGSLEAAVARAARAWIDD
                     LRDTLVAARGEEAGLDTLRRWNNAFPGAYQDDFGATDALADIVQLEQLTPEAPLAVRL
                     TMAGDHLDLNLYGLGAQPSLSEVLPRLTNLGVLVDDEHPYELTPLGLEPRWMKWFRLR
                     PPAGVAVDPSAFKLFEAAFLAIVNGRAEDDGFNRLVLSAGLAWREVALVRAYCRYYRQ
                     TGTLFSQSYIEDTLARHSHIARSIIELFIARLDPTAAETGAGDDPEAVADEIRTALDA
                     VTSLDDDRILRALLNLVNATLRTNWFQDEPDTVALKFDPTQIPDLPLPRPLYEIWVYS
                     PRVEGVHLRSGKVARGGLRWSDRREDFRTEVLGLMKAQRVKNAVIVPAGAKGGFVVKR
                     PPADPAELRNEVEACYRLFVGSMLDITDNLIDGRVIPPRAVVRYDGDDPYLVVAADKG
                     TATFSDIANEIALARGFWLGDAFASGGAHGYDHKAMGITARGAWESVKRHFRHLGIDP
                     DADDFTVVGIGDMSGDVFGNGMLLSEHIRLVAAFDHRHVFLDPGPDAAQSFAERKRLF
                     ELPRSSWDDYDKTLISAGGGVYPRTLKSITITPEVRAALGLDESVTACTPAELIRAIL
                     KAPVDLLFNGGIGTYVKAHTETNADVGDKASDAVRIDGRELRARSVAEGGNLGLTQLG
                     RVEYALNGGRINTDAIDNSAGVDTSDRE"
BASE COUNT          351 a          769 c          690 g          321 t
ORIGIN      
        1 cacccgcttg tcggcctcgg tgctcgctcg attgcactac gtcctgcacg tcgatccgac
       61 cgaccggcac gaggtcgacc tcgggagtct cgaagccgcg gtggcgcgag cggcgcgcgc
      121 ctggatcgac gatctgcgcg acacgctcgt cgcggcgcgc ggcgaagaag cagggctcga
      181 cacgctccgc cgctggaaca acgccttccc cggcgcctac caggacgact tcggcgcgac
      241 cgacgccctg gccgacatcg tgcagctcga acagctcacg cccgaagcac cgctcgcggt
      301 ccgcctcacg atggccggcg atcatctcga tctcaatctc tatggtctgg gcgcgcagcc
      361 gtcgctctcc gaggtgctgc cccgactcac gaacctcggc gtgctcgtcg acgacgaaca
      421 cccctacgaa ctcacgccgc tcgggctcga accgcgctgg atgaagtggt tccggctgcg
      481 gccgcccgcc ggtgtggccg tcgatccctc ggccttcaag ttgttcgaag cggcgttcct
      541 cgcgatcgtg aacggccgcg ccgaggacga cgggttcaac cggctcgtgc tcagcgccgg
      601 actggcgtgg cgcgaagtcg cgctcgtgcg cgcgtactgc aggtactacc gccagaccgg
      661 gacgctgttc agccagagct acatcgagga cacgctcgcc cgccattcgc acatcgcacg
      721 cagcatcatc gagctgttca ttgcgcgtct cgatccgact gcggccgaga ccggtgccgg
      781 cgacgatccc gaagcggtgg ccgacgagat ccgcacggca ctggacgcgg tcacgagcct
      841 cgacgacgac cgcatcctgc gcgcgctcct caacctcgtg aacgcgacgc tgcgcacgaa
      901 ctggttccag gacgaacccg acaccgtcgc cctgaagttc gaccccactc agattcccga
      961 cctccccctt ccgcggccgc tgtacgagat atgggtgtac tcgccgcgtg tcgaaggtgt
     1021 gcatctgcgc tccggcaaag tcgcacgcgg cggcctgcgc tggtcggacc ggcgcgaaga
     1081 cttccgcacg gaagtactcg ggctgatgaa ggcccagcgt gtgaagaacg cagtgatcgt
     1141 gcccgccggc gccaagggcg gcttcgtcgt gaagcgcccg cccgccgatc ccgcggagct
     1201 tcgcaacgaa gtcgaagcct gctaccggct gttcgtcggc tcgatgctcg acatcaccga
     1261 caacctcatc gacggccgtg tcatcccgcc tcgcgcagtg gtgcgctacg acggcgacga
     1321 tccgtatctc gtggtcgcgg ccgacaaggg aaccgcgacg ttctccgaca tcgcgaacga
     1381 gatcgcgttg gcgcgcgggt tctggctcgg ggacgcgttc gcgtcgggcg gcgcgcacgg
     1441 ctacgaccac aaggcaatgg gcatcacggc gcgcggcgcg tgggagtcgg tgaagcggca
     1501 cttccgccac ctcggcatcg acccggacgc cgacgacttc acggtggtcg gcatcggcga
     1561 catgtcgggc gacgtgttcg gcaacggcat gctgctctcc gagcacatcc ggctcgtcgc
     1621 cgcgttcgac caccggcacg tgttcctcga tcccggcccc gacgccgcgc agtcgttcgc
     1681 agagcgcaag cggctgttcg aactcccccg gtcctcgtgg gacgactacg acaagacgct
     1741 gatctcggcc ggcggcggcg tgtatccgcg cacgttgaag tcgatcacga tcactcccga
     1801 ggtgcgcgcc gccctcggcc tcgacgagtc ggtcaccgcg tgcacgccgg ccgaactgat
     1861 ccgcgccatc ctgaaggcgc ccgtcgacct gctgttcaac ggcggcatcg gcacctacgt
     1921 caaggcgcac accgaaacga acgccgacgt cggcgacaag gccagcgacg cggtccgcat
     1981 cgacggccgc gagttgcgcg cccggtcggt cgccgaaggt ggcaatttgg ggctcacgca
     2041 gttgggacgg gtcgagtacg cgctgaacgg cggacggatc aacaccgacg cgatcgacaa
     2101 cagcgccggc gtcgacacca gcgaccgcga a
//
LOCUS       DASUGA010000490         5824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1353135, whole genome shotgun sequence.
ACCESSION   DASUGA010000490 DASUGA010000000
VERSION     DASUGA010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353135"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..686)
                     /locus_tag="VFX21_09610"
     CDS             complement(84..686)
                     /locus_tag="VFX21_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096260.1"
                     /translation="MIVAAVDPDQARADAEAILGGRRYHSDPAPRPFRGQLKWIGDRL
                     QSVGDAIGKVFDLLPWWVWLLIAITAVALFVWYLVRNAERRRVLRVAAGTAPSATVAT
                     DDPDALERDADDAERNGDYERAVRLRFRAGLLRLGTRGRIEYTSSITNGEVRRTLQSP
                     TFDDLASTFDEITYGEREAVPPDASDARTRWPELTRGGRR"
     gene            complement(683..1513)
                     /locus_tag="VFX21_09615"
     CDS             complement(683..1513)
                     /locus_tag="VFX21_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096261.1"
                     /translation="MVQELRARGVGEILDVAVALYRARFATLMKISVIFVLPLSLFQM
                     FVFLSALPDDYTVANNGTLTPRYNGAEDQLLNLAALLVTVLVSTLGSAIVTAASTKVV
                     ADAYMSTAGPTESPVKASFRRILAIFGIALLFTVVTSFCPLAGYFIVTLWAVALPALM
                     LERLSGSRALSRSYEMTKGHFGLSLGVYWGSQILVSVLTLGSAGLLTLLVFTGNHSVT
                     TEVVTQSVANAVMSVVMTPFAACALVALYFDLRIRDEAYDVQAMIARLDRRQADAYRA
                     "
     gene            complement(1552..2736)
                     /locus_tag="VFX21_09620"
     CDS             complement(1552..2736)
                     /locus_tag="VFX21_09620"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX5096262.1"
                     /translation="MSGSVIVGLARTPIGKLSGAFAGQTAQDLGGFAIKAALERAGVG
                     PEQVDYVLMGQVLQAGQGQITARQAAAKAGIPMTVPAMTVNKVCLSGINAIYLADQMI
                     AAGDAEVVVAGGMESMTQAPYLLPGARAGYRMGNGELVDSLINDGLWCAFDAVHMGSG
                     TENYVGQFGGITREQQDELAAKSHERAAAAQKEGRFADEIAPVSLPQRKGDPIVIDTD
                     EGVRPGTTTESLSGLRPAFSKDGTITAGNASQISDGGAAVVMMSKAKADELGVTPLAE
                     LLGYGMVAGPDPSLLTQPSRSIKRALESIDLSVKDIDLFELNEAFAAVGIASMKDLGI
                     SDDVVNVNGGAIALGHPVGMSGTRVVMTVINELRRRGGGVGAAALCGGGGQGDAAIIR
                     TL"
     gene            2825..4165
                     /gene="ccrA"
                     /locus_tag="VFX21_09625"
     CDS             2825..4165
                     /gene="ccrA"
                     /locus_tag="VFX21_09625"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019521793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HEX5096263.1"
                     /translation="MSIEAIRTAILEGAPADEIGSLSIPASVRGALVKADETEMFAGL
                     ASEEKDPRKSTHVEEFPLPELAPDEAFVAVMASAINFNTVWTSIFEPLPTFGFLQRLG
                     KESVWGKRHDQPFHVMGSDLSGVVLKTGSAVRNWKAGDRVTAHCLHVDDQDPSAHDDS
                     MMATNQRIWGFETNFGGLAEIAVVKANQLMPKPAHLSWEEAACNALCNSTSYRMIVSP
                     NGSQMTQGQAVLVWGATGGIGAYAVQYVLNGGGVPVGVVSSDKRAALLREIGCEHVID
                     RREENYKFWKDDTEQDPKEWLRLGKKVRELIGRDVDIVFEHPGRQTMGASVFVAKRGG
                     MIITCAATSGYKIEYDNRYLWMNLKTLKGCHFANYREAYEANRLVCEGRIHPTLSKTY
                     SLDDTGEAAYQVHHNLAEGKIGVLCLAPEEGLGVTDEELRAKHIDQITLFRRHA"
     gene            4162..4569
                     /gene="mce"
                     /locus_tag="VFX21_09630"
     CDS             4162..4569
                     /gene="mce"
                     /locus_tag="VFX21_09630"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX5096264.1"
                     /translation="MTALFTEIDHIAIVVNDLEGAIDYYRSTYGCEVDHREVVEKDGV
                     EEALLKVADSYIQLLTPVRDDSQVAKYLANKGEGLHHVGYRVKDCAEALASVKREGHR
                     VIDEAPRPGSRGTTVAFIHPKTAFGTLIELVQE"
     gene            4582..5706
                     /locus_tag="VFX21_09635"
     CDS             4582..5706
                     /locus_tag="VFX21_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096265.1"
                     /translation="MPLPSPAEALQRPPDAEEVRVIVGGVVGAVSPDGQLTHLQRLLI
                     EAISESMTGFLAPAAAVPRLSPQEFAHAMAHRDAGFRSRMVQFMLLGEMVLVPLPAAV
                     AARVEEYAAELGVDNSMLRVARRYVSGSLGLALIDFQRSGYDWSPDRSAHLHTTSALE
                     EAWELACHDPALVERWARLSDCPDGSLGLAVSRFYAARGFTFPGSPSSAPPLLAQHDW
                     VHVLADYGSTVEAEIETFGFISRANDEPRGFALLAMVLGLFETGYIGSAAGLFAYDTG
                     HLSNVGMPARLADAMRRGALCAAHAGNGVDFLDVDWFSYADRPIDEVRAEFGLVPKAE
                     RAIEAGSVGPWERGGISPYQYETARAAAGDAYDSYGASPV"
BASE COUNT          995 a         1922 c         1998 g          909 t
ORIGIN      
        1 tccgcgggca cccaggttga ccgcgacgac gaccacgacc gcgatcacca cgacgagcac
       61 gacggtgcgg gtgcgactgt cggtcatcgg cgaccgccgc gcgtcagctc gggccagcgg
      121 gtgcgggcat cggacgcgtc gggcggcacc gcttcacgct cgccgtaggt gatctcgtcg
      181 aacgtcgacg cgaggtcgtc gaaggtgggt gactgcaacg tccgacgcac ttcgccattg
      241 gtgatcgacg aggtgtactc gatgcgaccg cgcgtgccga gtcgcaggag gccggcccgg
      301 aatcgcagcc gaacggcacg ctcgtagtcg ccgttgcgtt cggcgtcgtc ggcgtcgcgt
      361 tcgagtgcat cggggtcgtc ggtcgcgacc gttgcggagg gcgcggtccc ggccgcgacg
      421 cgcagcacgc gccggcgctc cgcgtttcgc acgaggtacc agacgaacag ggcgacggca
      481 gtgatcgcga tgagcagcca cacccaccac ggcagcaggt cgaagacctt cccgatcgcg
      541 tcacccaccg actgcagccg atcgccgatc cacttcagct gcccacggaa cggacggggt
      601 gcggggtcgc tgtggtaacg ccgtccgccg aggatggctt cggcgtcggc acgcgcttgg
      661 tcgggatcga cggcggcgac gatcacgcgc ggtacgcgtc tgcttggcgg cgatcgagcc
      721 gagcgatcat ggcctggacg tcataggcct cgtcacggat gcgcaagtcg aagtacagcg
      781 cgacgagcgc gcacgccgcg aacggcgtca tgacgacact catcaccgcg ttcgcgaccg
      841 actgcgtcac cacctcggtc gtcaccgagt gattgccggt gaacaccaac agcgtgagca
      901 ggcccgcgga accgagcgtc aacacgctca cgaggatctg gctgccccaa tagacgccga
      961 gcgacaaccc gaaatgccct ttcgtcatct cgtacgaccg gctcaaggcg cgcgaccccg
     1021 acaggcgctc cagcatcagc gccggcagcg ccacggccca caacgtcacg atgaaatatc
     1081 cggcgagggg acagaacgag gtgacgacgg tgaagagcag ggcaatcccg aagatcgcca
     1141 ggatgcgccg gaagctcgcc ttgacgggtg actcggtcgg gccggcggtg gacatgtacg
     1201 cgtcggcaac gaccttcgtc gacgccgcgg tcacgatcgc gctccccagc gtcgacacga
     1261 ggacggtgac gagcagcgcc gcgaggttga gcagctggtc ctcggcgccg ttgtaacgcg
     1321 gcgtgagcgt tccgttgttc gcgaccgtgt agtcgtcggg aagcgccgac aggaacacga
     1381 acatctggaa caacgacagg ggcagcacga agatgaccga gatcttcatc aacgtggcga
     1441 agcgcgcgcg atagagcgcg acggcaacgt cgaggatctc gccgactccg cgtgcgcgga
     1501 gctcctgcac cacgccgggc aggctaacgc ccggcgtggg gaggtttgga cctagagcgt
     1561 gcggatgatg gcggcgtcgc cttggccacc accgccgcac aacgccgcgg caccgacgcc
     1621 accaccgcgg cggcgcagct cgttgatgac cgtcatcacg acgcgcgtgc ccgacatgcc
     1681 gacggggtgg ccgagcgcga tggcgccacc gttgacgttc acgacgtcgt cggagattcc
     1741 caggtccttc atcgacgcga tgccgacggc ggcgaacgcc tcgttcaact cgaagaggtc
     1801 gatgtctttg accgacaggt cgatggactc gagcgcgcgc ttgatcgacc gtgacggctg
     1861 cgtcagcagc gacgggtcgg gtccggcgac catgccgtag ccgagcagtt cggccagcgg
     1921 tgtcacgccg agctcgtcgg ccttggcctt gctcatcatc acgactgcag cgccgccgtc
     1981 ggagatctgc gacgcgttgc cggccgtgat ggtgccgtcc ttggagaagg cgggccgcag
     2041 gccactgagc gactcggtcg tcgtgcccgg gcgcacacct tcgtcggtgt cgatgacgat
     2101 cgggtcaccc ttgcgctgcg gcagcgagac cggcgcgatc tcgtcggcga agcggccttc
     2161 tttctgcgcg gccgccgcgc gctcgtggct cttggccgcg agctcgtcct gctgttcgcg
     2221 cgtgatgccg ccgaactgac ccacgtagtt ctcggtgcca ctgcccatgt gcaccgcgtc
     2281 gaacgcgcac cacaggccgt cgttgatcaa cgagtcgacg agctcaccgt tgcccatgcg
     2341 gtagcccgca cgcgcgccgg gcagcaggta cggcgcctgc gtcatcgact ccatgccgcc
     2401 ggcgacgacg acttccgcgt cgcccgccgc gatcatctgg tcggcgaggt agatggcgtt
     2461 gatgcccgag aggcacacct tgttcacggt catcgcgggc acggtcatcg ggatgccggc
     2521 cttcgccgcg gcctggcgcg cggtgatctg tccctggccc gcctggagca cttgccccat
     2581 gagcacgtag tcgacttgct cgggaccgac acccgcccgc tcgagcgcgg ccttgatagc
     2641 gaagccgccg aggtcttggg ccgtctgacc cgcgaacgcg ccggacagtt tgccgatcgg
     2701 cgtacgcgcc aatccgacga tcaccgaacc ggacatgggt ccctcctagg acgagcggtg
     2761 agagcgaagc gtcagtgtag ggatgggtgg tttggtttcg ctactggccg gtagcttcac
     2821 tcgcatgagc atcgaagcga tccgtaccgc gatcctcgaa ggcgcacccg ccgacgagat
     2881 cggaagtctg tcgatcccgg cgtccgtgcg gggcgcgctg gtgaaggccg acgagacaga
     2941 gatgttcgcg gggctcgcat cggaggagaa ggatcctcgg aagtcgacgc acgtcgagga
     3001 gttcccgctg cccgagctgg cgcccgacga ggcgttcgtc gcggtcatgg cgtcggcgat
     3061 caacttcaac acggtgtgga cgtcgatctt cgagcccttg ccgacgttcg ggttcctcca
     3121 gcggctcggc aaggagtcgg tgtggggcaa gcgtcacgac caacccttcc acgtcatggg
     3181 ctccgacctc tcgggcgttg tcttgaagac cggttccgcg gtccgcaact ggaaggccgg
     3241 cgaccgggtc accgcgcact gcttgcacgt cgacgatcaa gacccgagcg cgcacgacga
     3301 ctcgatgatg gcgacgaacc agcgcatttg ggggttcgag acgaacttcg gcgggctcgc
     3361 cgagatcgcg gtggtgaagg caaaccagct catgccgaag cccgcgcacc tttcgtggga
     3421 agaggcggcg tgcaacgcgc tgtgcaacag tacgagctac cgcatgatcg tcagccccaa
     3481 cggctctcag atgacgcagg ggcaggcggt cctggtgtgg ggtgcgaccg gcggcatcgg
     3541 cgcgtacgcg gtgcagtacg tgttgaacgg cggcggtgtc cccgtcggtg tcgtgtcgag
     3601 tgacaagcgg gcggccttgt tgcgcgagat aggctgcgag cacgtcatcg accggcgcga
     3661 agagaactac aagttctgga aggacgacac cgagcaggac ccgaaggagt ggttgcggct
     3721 cgggaagaag gtccgtgagc tcatcgggcg cgatgtcgac atcgtgttcg agcacccggg
     3781 tcgacagacg atgggcgcgt cggtgttcgt cgccaagcgc ggcggcatga tcatcacgtg
     3841 cgcggccacc tcgggctaca agatcgagta cgacaaccgc tacctctgga tgaacctgaa
     3901 gacgctcaag ggctgccact tcgcgaacta ccgcgaggcg tacgaagcga accgcctcgt
     3961 gtgcgaaggc cgcatccacc cgacgctgtc gaagacctac tcgctcgacg acaccggtga
     4021 agccgcgtac caggtgcacc acaacctggc cgagggcaag atcggtgtcc tctgcctcgc
     4081 acccgaagaa gggctcggcg tgaccgacga agagctgcgc gcgaagcaca tcgaccagat
     4141 cacgctcttc cggaggcacg catgacggcg ctcttcaccg agatcgacca catcgcgatc
     4201 gtcgtcaacg acctcgaagg ggcgatcgac tactaccgtt cgacgtatgg ctgcgaggtc
     4261 gaccaccggg aggtcgtcga gaaggacggc gtcgaagaag cgctactcaa ggtggccgac
     4321 tcgtacatcc agctgttgac gccggtgcgc gacgactcac aggtcgcgaa gtacctcgcg
     4381 aacaagggcg aagggctgca ccacgtgggc taccgggtga aggactgcgc ggaagcgttg
     4441 gcatcggtga agcgcgaagg ccaccgcgtc atcgacgaag cgccgcgtcc gggaagtcgc
     4501 ggcacgacgg tcgcgttcat ccatccgaag accgccttcg gcacactgat cgagctcgtc
     4561 caggagtaac tgcctatgcc ggtgccgctt ccgtccccgg ccgaagccct gcagcgtccg
     4621 cccgacgccg aagaagtacg cgtcatcgtc ggcggtgtcg tcggtgccgt ctcgcccgac
     4681 ggtcagctga cgcacttgca gcgattgctg atcgaggcga tcagtgagtc gatgactgga
     4741 ttcctcgcgc cggcggccgc ggtgccccga ctgtcaccgc aggagttcgc gcacgcgatg
     4801 gcacaccgcg acgcggggtt ccgcagtcgc atggtgcagt tcatgttgct gggcgagatg
     4861 gtactggtgc cgctgcccgc agcagtggct gcgcgcgtcg aggagtacgc agccgagctc
     4921 ggcgtcgaca actcgatgct gcgcgtcgcg cgccgttacg tgagcggcag cctcgggctc
     4981 gcgctgatcg acttccaacg cagcggctac gactggagcc cggatcgctc cgcgcatctc
     5041 cacacgacct ccgcgctgga ggaagcgtgg gagttggcgt gccacgatcc ggcgctcgtc
     5101 gagcggtggg cgcggctctc cgactgtccg gacggatcgc tcggcctcgc ggtgtcgcgc
     5161 ttctacgcgg cgcgcggttt cacgttcccc ggctcgccga gcagtgcgcc gccactgctc
     5221 gcgcagcacg actgggtgca tgtgctcgcc gactacggct cgacggtcga ggccgagatc
     5281 gagacattcg ggttcatctc gcgcgcgaac gacgaaccgc gcggcttcgc gctgttggcg
     5341 atggtcctgg gcttgttcga gaccggatac atcggctccg ccgccggcct cttcgcgtac
     5401 gacaccggcc acttgtcgaa tgtgggcatg ccggcccgcc tcgcggacgc aatgcggcgc
     5461 ggcgcgctgt gtgctgcgca cgccggtaac ggtgtcgact tcctcgacgt cgactggttc
     5521 tcctatgccg accggccgat cgacgaggtg cgcgccgagt tcgggctcgt tccgaaggcc
     5581 gagcgcgcga tcgaagcggg ctcggtcggg ccgtgggaac gaggcggtat atcgccgtac
     5641 caatacgaga ccgcgagggc cgccgccggc gacgcgtacg actcctatgg ggcttccccg
     5701 gtctgaagca tcacaagcgg gtgcggatag ccttgcccca tggctgcaac cgacggcggc
     5761 gcgccgccgc cgactccgat ccccctccag gaccgtctcg acgagctcgc caagctgaag
     5821 gaag
//
LOCUS       DASUGA010000491         4367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_432037, whole genome shotgun sequence.
ACCESSION   DASUGA010000491 DASUGA010000000
VERSION     DASUGA010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432037"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /gene="rsmH"
                     /locus_tag="VFX21_09640"
     CDS             complement(<1..846)
                     /gene="rsmH"
                     /locus_tag="VFX21_09640"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX5096266.1"
                     /translation="MKAEFSHLPVMVREVVELLLPVPAGLVVDCTLGGGGHSAALLDA
                     RPDITVLGIDRDADAVAAARSRLEVYGPRVKAVHGGFEALGDLVARHGEGAQVMGILM
                     DLGVSSPQLDRADRGFSYRYDAPLDMRMDAKQTLTAADVVNGYDEEELAGVIAQFGEE
                     RFARRIARAIVAARPLHSTRELAEIVRDAIPAATRRRGPHPARRTFQAIRMEVNRELP
                     NLAAGLDESVHVIGPNGRVLVLSYHSLEDRMVKQHFARWAGRDADAQAETLAKLPVEP
                     VRRRPL"
     gene            complement(1076..1501)
                     /gene="mraZ"
                     /locus_tag="VFX21_09645"
     CDS             complement(1076..1501)
                     /gene="mraZ"
                     /locus_tag="VFX21_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX5096267.1"
                     /translation="MFLGEHQHSLDAKGRVILPARFRDQLEGGAVMARALDGCLAVYT
                     LDEFQRVAAKLAAARERGPRERQAARSVFTGAVDFTPDKQGRVAIPTHLREYAGLDRE
                     VVVAGGYDHIEIWDAQRFGERDQEGIASIVGGEGIDDFM"
     gene            complement(1676..2185)
                     /locus_tag="VFX21_09650"
     CDS             complement(1676..2185)
                     /locus_tag="VFX21_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006541435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX5096268.1"
                     /translation="MARALLLNASFEPLCVVSSRRAVVLVLKDKAEIVHRNGAEFRSE
                     RRSVPVPTVIRLVHFVRVPFRATAPLSRRAVFARDGHRCQYCGSNAENLDHVVPRSRG
                     GRHTWDNVVASCRACNARKEDRLPAECGMVPKRLPVAPHATTSLIASAGPLDPDWQPY
                     LSRIHAVPA"
     gene            2580..3554
                     /locus_tag="VFX21_09655"
     CDS             2580..3554
                     /locus_tag="VFX21_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015032954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5096269.1"
                     /translation="MVHAAPRPDTSFKDTFDAIARQVETVLRGKRRAVHLSLVCLFAE
                     GHLLIEDVPGVGKTSLAKAIARSTSGTWRRIQFTPDLLPSDVTGASVWDRSRNDFEFR
                     SGAVFANIVLADEINRASPKTQSALLESMEERQVTVDATTYKLPTPFMVIATQNPVEL
                     EGTYPLPEAQLDRFLLRLRIGYPDRDAELAILDAQGTQVVDVDELEPVTDSETVAFWS
                     GELARVHIAPELQGYVVDLADATRHHRDLMLGVSPRGALALQRAGRALAASVGRSYVV
                     PDDIKALAPAVLEHRLVLSPEAMMRGVSSADVLTSVLESIPVPATRLD"
     gene            3565..>4367
                     /locus_tag="VFX21_09660"
     CDS             3565..>4367
                     /locus_tag="VFX21_09660"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5096270.1"
                     /translation="MLTRRGWSLLGASLGLYVGSRILGLVQMVVLAAIGVLLLVGAAI
                     WVRLQRLDLLAGRHLRERLQVGVEGRVDVAIEAAGRTPTVTVSDAFDRGRRSARFLLS
                     PLRPGESARAAYRIPTERRGRFELGPLRATVSDPFGLASRTERVLRSEEVIVYPRVFD
                     VMSLPEAGGTELDRDQPRAHGRLDPGGEFLTLRDYEPGDDLRRVHWRSTARRDRLMVR
                     QNEARRRAPVLLMLDVRPGAHDRGSFERAVEACASVVTVLDRIGRPCEV"
BASE COUNT          646 a         1514 c         1536 g          671 t
ORIGIN      
        1 gaggggccgg cggcgcacgg gctcgaccgg gagcttggcg agggtctcgg cctgtgcgtc
       61 ggcgtcccgg ccggcccatc gtgcgaagtg ttgcttcacc atccggtcct cgagcgagtg
      121 atacgagagg accagcactc ggccgttggg gccgatgacg tgcaccgact cgtcgagacc
      181 ggccgcgagg ttgggaagct cgcggttcac ctccatgcgg attgcttgga acgtccggcg
      241 cgccgggtgc ggcccgcgcc ggcgcgtcgc cgccgggatc gcgtcgcgca cgatctccgc
      301 gagctcgcgc gtcgaatgca acggacgcgc ggcaacgatc gcgcgcgcaa tgcgccgcgc
      361 gaagcgctcc tcaccgaact gcgcgatgac gccggcgagc tcctcttcgt cgtaaccgtt
      421 gacgacgtcg gcggcggtca gcgtctgctt cgcgtccatg cgcatgtcga gcggcgcgtc
      481 gtagcggtac gagaacccgc gatcggcgcg atcgagctgc ggactgctca cgccgaggtc
      541 catcaggatt cccatgactt gcgctccctc accgtgccgg gcgaccaggt cgccgagtgc
      601 ttcgaacccc ccgtgcactg ccttcacgcg aggtccgtag acctcgagcc gcgagcgggc
      661 ggcggcaacg gcgtcggcgt cacgatcgat cccgagcacg gtgatgtcgg gtcgcgcgtc
      721 caggagtgcg gccgagtggc cgccgccccc gagcgtgcag tcgacgacga gaccggccgg
      781 gacaggaagg agcagttcca ccacctcccg gaccatcacc ggaagatgcg agaactccgc
      841 cttcatgacc cgaccgcgag gttcgggacc gcagggttcg gatcgatcat ggcgccggag
      901 ccgctcccgc cgccggactc gtctccagcc tcccggtcgg caggcgccgc tcgggatgtc
      961 tccccgagtc ggctacggct gaaagcgggc ggagtagggg ccaacaaccg cgccggccgg
     1021 gaggctggag acgggcccgg cgaaccgggc tccgttatgt caagggtgtg cggcatcaca
     1081 tgaagtcgtc gatgccctct cctccgacga tggaggcaat tccttcttgg tcgcgctcgc
     1141 cgaaacgttg cgcgtcccag atctcgatgt ggtcgtagcc accggcgacc acgacctcgc
     1201 ggtcgaggcc ggcgtactcg cgcaggtggg tcgggatcgc gacccgcccc tgcttgtcgg
     1261 gggtgaagtc gaccgcgccg gtgaagacgg agcgggcggc ctggcgctcg cggggaccgc
     1321 gctcccgggc ggcggccagc ttggccgcga cccgctggaa ctcgtcgagc gtgtagacgg
     1381 caaggcagcc gtcgagcgcc ctggccatca ccgcaccccc ttcgagctgg tcccggaaac
     1441 gcgccgggag gatgacgcga cccttggcgt ccagggagtg ctggtgctcc cccaagaaca
     1501 tctgtcgcgc accacctgac atccctcagt gtggggaagt cctccccttt ccaccaccag
     1561 gcgccaccct aagccactct cccccaccag tcaacacact ccagcccctt tggcatcaca
     1621 aaaaacagca gccgcacccg tgggtgcggc tgaagcgccc gaaatggggg ttcggtcagg
     1681 ccgggacggc gtgaattcgg ctcaggtagg gctgccagtc gggatcgagg gggccggcgc
     1741 tggcgatcag cgaagtggtg gcatggggtg ccaccgggag gcgcttgggc accatcccgc
     1801 actcggccgg gagccggtcc tccttgcgcg cgttgcaggc gcgacaggac gcgacgacgt
     1861 tgtcccaggt gtggcggccg ccccgcgacc gcggaacgac gtggtcgagg ttctcggcgt
     1921 tgctgccgca gtactgacag cggtgcccgt cgcgcgcgaa gacggcccgg cgggacagcg
     1981 gcgccgtcgc gcggaacggg acacgcacga aatggacgag gcggatcacc gtcgggaccg
     2041 ggacgctgcg ccgctcggaa cggaactcgg ccccgttgcg gtgcacgatc tcggccttgt
     2101 ccttgaggac gaggacgacc gcgcggcgtg acgacacgac acagagcggt tcgaagctgg
     2161 cgttcagcag cagcgcccga gccacggcga cggtcacctc cagtgccccg aaaggcacgg
     2221 gctcggcgcc cttcggtgtc cggagtctac cgagacgacc ggcgcaccca ggcgagatag
     2281 tcgacgactt cttcgggttt tggttcaccg ttcgttccgt acgcggtctg catgcgaaac
     2341 gccagatact cacgatcggg gaccgggagg aacggtggac gtcgccacca cccggggcgg
     2401 gcgatgcgag cgagttgacg cagcgcggtg aaccagagcc acgggcgaac cgttatccgc
     2461 agcagggtat ggaaccaaaa cgagcgctcg agcgtcactt ctcgttgacc gtcggcaggg
     2521 ttcgggttca ccgaccggga cggtagcgtt tcggcgtctg gcccctcatc ggaggtttca
     2581 tggtgcacgc ggctcctcgc ccggacacat ccttcaagga caccttcgac gcgatcgccc
     2641 gtcaggtcga gacggtgttg cggggcaagc gccgggccgt gcatctctcg ctggtgtgcc
     2701 tgttcgccga aggtcacctg ctgatcgaag acgtcccggg tgtcgggaag acgtcactcg
     2761 ccaaagcgat cgcgcgctcg accagcggca cgtggcggcg catccagttc acccccgacc
     2821 tgctgccttc cgacgtcacc ggcgcgtcgg tctgggaccg gtcgcgcaat gacttcgagt
     2881 tccgctcggg cgccgtgttc gcgaacatcg tgctggccga cgagatcaac cgcgcgtcgc
     2941 ccaagaccca gtccgcgctg ctcgaatcga tggaagagcg gcaggtcacc gtggacgcga
     3001 cgacgtacaa gctgccgaca ccgttcatgg tgatcgcgac gcagaacccc gtcgagctcg
     3061 agggcacgta cccgttgccc gaggcgcagc tcgaccggtt cctgctgcgg ttgcgaatcg
     3121 gttaccccga ccgtgacgcg gagctcgcga tcctcgacgc gcagggcacg caagtggtcg
     3181 acgtcgacga gctcgaaccg gtcaccgact cggagaccgt tgcgttctgg tccggcgagc
     3241 tcgcgcgcgt gcacatcgcg cccgagctcc agggctacgt cgtcgatctt gccgacgcaa
     3301 cgcgccacca ccgcgatctc atgctcggcg tgagcccgcg cggcgcgctc gcgttgcagc
     3361 gcgcgggtcg tgcactcgcc gcgagcgtcg gccgttcgta cgtcgtgccc gacgacatca
     3421 aggcgttggc gcccgcggtg ctcgagcacc gtctggtgct gtcgccggag gcgatgatgc
     3481 gcggggtcag cagcgccgac gtgttgacgt cggtactgga gagcatcccc gtgcccgcca
     3541 ctcggctcga ctagtggggc gctcgtgttg accaggcgcg gctggtcgct gctgggggca
     3601 tcgctcgggc tgtacgtcgg ctcgcggatc ctcgggctcg tgcagatggt ggtgctggcc
     3661 gcgatcggcg tgttgttgct ggtcggcgcc gcgatctggg tgcgcctgca gcgcctcgac
     3721 ctgctcgccg gccgtcacct gcgcgaacgg ttgcaggtcg gcgtcgaagg acgtgtcgac
     3781 gtcgccatcg aagccgccgg gcgcacaccg accgtcaccg tgtcggacgc gttcgaccgc
     3841 ggccgccgtt cggcccgctt cctgttgtcg ccgctgcgcc cgggcgagtc ggcgcgggcc
     3901 gcgtatcgca tcccgaccga acggcgcggc cgcttcgagc tgggcccgtt gcgggccacg
     3961 gtgtccgatc cgttcgggct cgcgagccgc accgagcgcg tcctgcgttc cgaggaagtg
     4021 atcgtctacc cgcgcgtctt cgacgtgatg tcactgcccg aagcgggcgg caccgagctc
     4081 gaccgcgacc aaccgcgcgc gcacggccgc ctcgatcccg gcggcgagtt cttgacgttg
     4141 cgcgactacg agcccggcga cgacttgcgc cgtgtgcact ggcgttcgac cgcgcgccgc
     4201 gaccggctca tggtgcgcca gaacgaagcg cgccgtcgcg caccggtgct tctcatgctc
     4261 gacgtgcgcc ccggcgcgca cgaccgcggc tcgttcgagc gcgcggtcga agcgtgcgca
     4321 tccgtcgtca ccgtgctcga ccgcatcggg cggccgtgcg aggtcat
//
LOCUS       DASUGA010000492         3839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_284690, whole genome shotgun sequence.
ACCESSION   DASUGA010000492 DASUGA010000000
VERSION     DASUGA010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284690"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VFX21_09665"
     CDS             complement(<1..251)
                     /locus_tag="VFX21_09665"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX5096271.1"
                     /translation="MQTTDVLEELKRLPMRPSSVSHALAILDDPNASAREVASALQTD
                     PTLCARLLHLANSPYFGLSGKVANIERAVVALGGSTVRS"
     gene            complement(356..913)
                     /locus_tag="VFX21_09670"
     CDS             complement(356..913)
                     /locus_tag="VFX21_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096272.1"
                     /translation="MAVLDVAGFVADLKDHAVEHGFHVHDERHFVESYSLRQTWEVDV
                     HPEEGCEGPVDLYLSLEIDPRVLLGFEDVVIGLPDDDEPEDAFHFPLSFTWALPPLPH
                     GPDLLVLATELAGIGGPDLPLEVSATDSFPSATDAPERSLRIVAHQRVSLLGIRRGEE
                     VPCEMLERCLGVSRYLLEHANDWLE"
     gene            complement(914..1843)
                     /locus_tag="VFX21_09675"
     CDS             complement(914..1843)
                     /locus_tag="VFX21_09675"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5096273.1"
                     /translation="MTLHPQARQLCDMVNAIGGTPASDEQLQQARDGLVMLHAGGAGE
                     APDVYAVGDLDADGVPVRVYRPSAADGLPVVVFLHGGGWTIGSVDCYDTLARTLANES
                     GATVVSVEYRLAPEHPFPAPLDDCFHALQWTAAHAGEFEADGSRLAIMGDSAGGNLAA
                     VCALMARNAGAPDLALQVLVYPVTDFSFATPSYAENGTGHVLSKEQMHWFWDCYTRGV
                     TDTDDWRLSPLRCADLSGAPPALVITAEHDPLRDEGEAYAEQLRDAGVAVEVTRYAGM
                     IHPFFALPGMMDDGKTAMAQVASALRLALKVGE"
     gene            complement(1840..2766)
                     /locus_tag="VFX21_09680"
     CDS             complement(1840..2766)
                     /locus_tag="VFX21_09680"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5096274.1"
                     /translation="MPLHPQAEVMLGAMSGAGFDLGPGADPADVRAVIDGMLGAAPGP
                     PVHRTEDRAAGSVPIRVYWPSDDAGLPILVWFHGGGWVIGSLDTHDGTCRNLCNDAGV
                     IVVSVDYRLAPEHKFPAAVDDCVAAWTWVNEHAAELGGDNTRVALGGDSAGGNLAAVV
                     SLIAREERLPVPALQLLVYPVTDYEFESPSMVDNATGYFLEAEGMRWFFGHYGRSGAD
                     YDDWRCCPLRAADHSSLPRAVVITAEFDPLRDQGEAYGRRLQQSGCDVEILRVDGVFH
                     GFFGMHAFLDPAQQPWDLTVASMQDVFGMGAA"
     gene            complement(2769..3455)
                     /gene="rdgB"
                     /locus_tag="VFX21_09685"
     CDS             complement(2769..3455)
                     /gene="rdgB"
                     /locus_tag="VFX21_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX5096275.1"
                     /translation="MSLPLPLVVATGNADKVSEIIEILVARTDEPLAAWALDVGDDTY
                     GYLVDAPAALAESVAAHGALATAPDVEETGTTLEANARIKATALCDATGLLAIADDTG
                     LEVDALEGAPGVQSARYAGADASYSDNVRLLLKELEGVYPKLRTARFATVALARGPDG
                     EEIAVRGEVAGIIAAAPKGEHGFGYDPVFVPTEGDGRTFAEMTPSEKHALSHRGRAFR
                     ALAEELTETR"
     gene            complement(3452..>3839)
                     /gene="rph"
                     /locus_tag="VFX21_09690"
     CDS             complement(3452..>3839)
                     /gene="rph"
                     /locus_tag="VFX21_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX5096276.1"
                     /translation="VLQADGGTRTASICGGWIALHDACSRLVTRGELSTHPVREACAA
                     ISVGVIDGVPMLDLEYVEDVRAEVDMNIVMTASERFVEVQGTAEGLAFTRPDLDALLD
                     LAQQGIAEIIAAQQEMVAVAPPVRGA"
BASE COUNT          601 a         1331 c         1326 g          581 t
ORIGIN      
        1 aacgagcgga ccgtcgatcc acccaatgcg acgacggcgc gttcgatgtt ggcgaccttg
       61 ccggacaacc cgaagtacgg agagttcgcg aggtgcaaca gccgcgcgca cagtgtgggg
      121 tccgtttgca gcgcggacgc gacctcgcgc gcgctggcgt tcggatcgtc gaggatggcg
      181 agcgcgtgcg aaaccgacga cggccgcatc ggcagccgct tcagttcctc caacacatct
      241 gtcgtctgca ccgctcccgc cccgttccct ggcctgggag tccaatcggt ccgaattggt
      301 accgggttga gcgtcagtac cggcagaagc ggacgccccg ggacgcggga ctgcgttact
      361 cgagccagtc gttggcgtgc tcgagcaggt accggctgac gcccaggcag cgctcgagca
      421 tctcgcacgg gacttcctcg ccgcggcgga tgccgaggag cgagacgcgt tggtgggcca
      481 cgatgcgcaa tgaccgctcg ggggcgtccg tcgcggacgg gaacgagtcg gtggcggaca
      541 cttcgagcgg caggtcggga ccgccgatac cggccagctc cgtcgcgagc acgagcagat
      601 cgggtccgtg cggcagcggt ggcagcgccc acgtgaagct caacggaaag tggaacgcgt
      661 cttcgggctc gtcgtcgtcg gggagcccga tgacgacgtc ctcgaagccg aggagcacgc
      721 gcgggtcgat ctccaacgac aggtagagat cgacggggcc ttcacaccct tcttcaggat
      781 gcacgtcgac ttcccacgtc tggcgcagcg agtagctctc gacgaagtgg cgctcgtcgt
      841 gcacgtggaa gccgtgctcg accgcgtgat ccttgaggtc ggccacgaac ccggccacgt
      901 cgagaactgc catctactcg ccaaccttca acgcgaggcg cagcgcgctc gcgacttgtg
      961 ccatcgcggt cttcccgtcg tccatcatgc cgggcagcgc gaagaacgga tgaatcatcc
     1021 cggcgtagcg ggtcacctcc accgcgacgc cggcgtcgcg cagctgttcg gcgtacgcct
     1081 cgccttcgtc gcgcagcggg tcgtgctcgg cggtgatgac gagcgccggt ggcgccccgc
     1141 tgaggtcggc gcaccgcaac ggtgacagcc gccagtcgtc ggtatcggtc acgccgcgcg
     1201 tgtagcagtc ccagaaccag tgcatctgct ctttgctcag cacgtgcccg gtgccgttct
     1261 ccgcgtacga cggggtggcg aagctgaagt cggtcacggg atacacgagc acctgcagcg
     1321 ccaggtcggg tgcgcccgcg ttgcgggcca tgagcgcgca gaccgcggcg aggttgccgc
     1381 ccgcgctgtc acccatgatc gcaaggcggg agccgtcggc ttcgaactcg cccgcgtggg
     1441 ctgcggtcca ctgcaacgcg tggaagcagt cgtcgagcgg tgcggggaac gggtgctccg
     1501 gcgcgagccg gtattcgacg gacacgacgg tcgcacccga ttcgttggcg agcgtgcggg
     1561 ccagcgtgtc gtagcaatcg acactgccga tcgtccaccc gccgccgtgc aggaatacga
     1621 cgaccggcaa gccgtcggcc gcggacggcc ggtacacgcg caccggcaca ccatccgcgt
     1681 cgaggtcgcc gaccgcgtag acgtcgggcg cttcaccggc accgcctgca tgcaacatca
     1741 cgagaccgtc gcgcgcttgt tgcaactgct cgtcggacgc gggcgtcccg ccgatcgcgt
     1801 tgaccatgtc gcagagttgc cgggcttgcg ggtgcagagt catgcagctc ccatcccgaa
     1861 gacgtcctgc atgctcgcca cggtgagatc ccatggctgc tgcgcgggat ccaagaacgc
     1921 gtgcatgccg aagaaaccgt ggaacacacc gtcgacgcgc aggatctcga cgtcacagcc
     1981 ggactgctgc agtcgccgtc cgtacgcttc gccttggtcg cgcagcggat cgaactcggc
     2041 ggtgatgacc acggcccgcg gcagcgacga gtggtcggcg gcgcgcaacg ggcaacaccg
     2101 ccagtcgtcg tagtcggcac cgctgcgccc gtagtgaccg aagaaccagc gcatcccttc
     2161 ggcttcgagg aagtaccccg tcgcgttgtc gaccatcgac gggctctcga actcatagtc
     2221 ggtgaccggg tagacgagca gctggagcgc gggaacgggc agccgttcct cgcgggcgat
     2281 caggctgacg accgccgcga ggttgccgcc cgcgctgtcg ccgccgagcg cgacgcgcgt
     2341 gttgtcgcca ccgagctccg ccgcgtgctc gttgacccat gtccacgcgg cgacgcagtc
     2401 gtcgaccgcg gccgggaact tgtgctccgg cgcgaggcgg tagtcgacgg agacgacgat
     2461 caccccggcg tcgttgcaca gattgcggca cgtcccgtcg tgggtgtcga gcgagccgat
     2521 gacccacccg ccgccgtgga accacacgag gatcggcagc cccgcatcgt cggacggcca
     2581 gtagacgcgg atcggcaccg agcccgcggc gcgatcctcg gtccggtgga ccggcggacc
     2641 gggcgccgcg ccgagcatgc cgtcgatgac ggcgcgcacg tcggccggat ccgcgccggg
     2701 tccgagatcg aacccggcac cggacatggc gccgagcatc acctcggcct gcgggtgcag
     2761 cggcatcgtt acctcgtctc ggtcagctct tcggcgagcg cacggaacgc gcgcccgcga
     2821 tgcgagagcg catgcttctc cgacggcgtc atctccgcaa acgtgcgccc gtcgccctcg
     2881 gtcggtacga agaccgggtc gtacccgaac ccgtgctcac ccttcggtgc cgccgcgatg
     2941 atccctgcga cctcgccgcg caccgcgatc tcctcgccgt cgggcccgcg cgccagcgcg
     3001 accgtcgcga accgggcggt gcgcagcttc ggatagacgc cttcgagctc cttgagcagc
     3061 aaccggacat tgtccgagta cgacgcgtcc gcgccggcgt agcgcgcgga ctgcacgccg
     3121 ggtgcgcctt cgagcgcgtc gacctcgagg ccggtgtcgt cggcgatcgc gagcagcccg
     3181 gttgcatcgc acaacgcggt ggccttgatg cgggcgttgg cttcgagcgt cgtccccgtc
     3241 tcttcgacgt cgggcgccgt cgcgagtgcg ccgtgcgcgg cgacggattc cgcgagtgcc
     3301 gccggtgcat ccaccagata gccgtaggtg tcgtcgccga cgtcgagcgc ccacgcggcc
     3361 agcggttcgt ccgtgcgcgc gacgaggatc tcgatgatct cgctgacctt gtcggcgttg
     3421 cccgtggcaa cgaccagcgg cagcggcagg ctcacgcgcc ccgaaccgga ggagcgacgg
     3481 caaccatctc ttgctgggcg gcaatgatct cggcgatgcc ctgctgcgcg aggtcgagca
     3541 gcgcgtcgag atcgggccgc gtgaacgcga gcccctccgc ggtcccctgc acttcgacga
     3601 agcgctccga cgcggtcatc acgatgttca tgtcgacttc ggcccgcacg tcttcgacgt
     3661 attcgagatc gagcatcggc acgccgtcaa tgacaccgac cgagatcgcg gcgcacgctt
     3721 cacgtacggg gtgtgtcgag agttcgccgc gggtgacgag ccgtgagcac gcgtcgtgca
     3781 gtgcgatcca cccaccgcag atggacgcgg tgcgggtgcc gccgtcggct tgcaggaca
//
LOCUS       DASUGA010000493         5304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1770579, whole genome shotgun sequence.
ACCESSION   DASUGA010000493 DASUGA010000000
VERSION     DASUGA010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1770579"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VFX21_09695"
     CDS             complement(<1..891)
                     /locus_tag="VFX21_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5096277.1"
                     /translation="MSTTDPRPAAPPATFAGFPMPRDPQALIDRSRRPIVRTATAEPR
                     FPFPVPNGWFIVAEARDLMPGETRAMYVFGRDVVLFRAADGAPHMVEAYCAHLGAHLG
                     AGGRVEGGCIRCPFHGWLYDGASGKCTEIPYGDMDRIPTQAKVRSFPCLERNEMIWAW
                     HHGDGGEPFYEVPDIPELRDDEWLPYEIVEFDVATCCQEMAENNVDLAHFMYVHGTDA
                     IPDDDFFVDGAYKRTVGADGNFVREGFGLGLGVLRVKGYTTFLSSTTPIDTENVKVRW
                     VFTAPAANGRDAARQAAEQFS"
     gene            1010..2344
                     /locus_tag="VFX21_09700"
     CDS             1010..2344
                     /locus_tag="VFX21_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006541766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5096278.1"
                     /translation="MADEKYRYAERMFPAPVDHGIAQPDRAVTSVPFGTSSYLEGGGG
                     AGNEWYEGGGALNEKAVAEIVVYPPTRGIATQGDPFEPVKIGILVDMDLGQLLADWID
                     PTILALEDALNEGTWGRSPVAIVTCDARGLPRENYRKLIEGYKWLVEQGCVVILGPMI
                     SDNCLTLQPYVEEIGVPVIGWTGAHKFASDYCYTVANGDIPTEGVMCANWLYQKGLKK
                     VGMFWEVGSSGRDYADFFRDEAIRLGLTITREVKLEPNPRNLKETLSDMRDMGTEGLY
                     YGGYGYATFHFADALNALDWDPPRVMGTAFMFYSNSNKWAEGLEGWHGVDQLGVDGTN
                     PNYEAMISRFEKRFGRKTRNVVVALAYDTARAAIHGIANAKIPIPREVRDGLDRIRWM
                     PATNGGPSCYIQFGPGDHKGYKGDFLTIRELRGGELRFDGYYRPEWPSNRTD"
     gene            2418..3641
                     /locus_tag="VFX21_09705"
     CDS             2418..3641
                     /locus_tag="VFX21_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5096279.1"
                     /translation="MARYAVISADGHAGPPAAVYRDYLDPGFRDRFDEHQRAMEELRE
                     AMGRSTPSEFQQEWEEETGGDGGLTAAYDSDARNAVLDEEGVIAEVLFPDADVLGTGR
                     VSSSPFGTGLAGASDSVDEAVAGSRAHNRWLADFVGQEPDRRIGVAVIPAIIPDIDLV
                     LQLVREAKELGHRGILIPTRWFDRPAYHDLCYEPLWSLTEELGLVLHTHSGAGPTDIG
                     LGPGMLPIYASEAGWWAARPLAVLIWGGIFERHPDLRYSMAENAAWWVPDQIRKMDEK
                     WIGGHNTRKFGDAFRQGLSMKPSDYLNRNCFFGASTPGEDDIDRRHLIGIGNIMWGND
                     LPHPEGTFPYTRYWIRKRFKDVPEDETRRMLGLTAAEVYGVDLDALAPLVERIGPTTE
                     EVHGDAPLARVPAGV"
     gene            3671..5029
                     /locus_tag="VFX21_09710"
     CDS             3671..5029
                     /locus_tag="VFX21_09710"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5096280.1"
                     /translation="MGHVRRMGALAAVAALTFAAGCTSESAGDDDDAASTQTTAAGDD
                     TGAATGAGNTAGITDTTIKISLLSSDLSALSEQNLAPEIGSADATMKAVVADINANGG
                     VAGRQIELVSHVIPGAEAIVNPDAGRQACVQATEDDQPFAVILAASLPASMVQCVAQD
                     HDPLTITMDSWPDSLYAAADGRLFSVGSQISVGMSRLFSAWPGILDDAGALEGKTVGI
                     IRQDTLSNDQKDAVDNSLKPALETLGYEVAAEAVLPCPETSQTCEQHQAAIQRMQDAG
                     VDFVFLVAQTLAGSTTVETAQSSGYKPTWATVGNNVTPTVAKFYTNARDNYEGAWGLS
                     VDFTDFTEAAANCNRIAVAGGADEFPTSSDGYGFTGVTCLQMQILAQAIDRVDGSLDQ
                     ASVIAALESLGDDVPAISGGPTSLSKDKHDAGNAVFVARYSAATEKFEPVDDRKPVEV
                     PE"
     gene            5047..>5304
                     /locus_tag="VFX21_09715"
     CDS             5047..>5304
                     /locus_tag="VFX21_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096281.1"
                     /translation="MPDPEAPDAEPEATAALAAAVLDEELRRAEAVVQPDVVLADNEL
                     PGVDDPAMSLRDALRVGGSRTIVIVGVLGALALVDNGIFNVL"
BASE COUNT          914 a         1749 c         1824 g          817 t
ORIGIN      
        1 cgagaactgt tcggcggcct ggcgcgccgc atcgcggccg ttcgccgccg gcgcggtgaa
       61 gacccagcgc accttgacgt tctcggtgtc gatcggtgtc gtggacgaca ggaacgtcgt
      121 gtagcccttg acgcgcagga cgccgaggcc caggccgaat ccctcgcgta cgaagttgcc
      181 gtcggcgccg acggtgcgct tgtacgcgcc gtcgacgaag aagtcgtcgt cgggaatcgc
      241 gtcggtgccg tgcacgtaca tgaagtgcgc taagtcgacg ttgttctccg ccatctcctg
      301 acagcacgtc gcgacgtcga actcgacgat ctcgtagggg agccactcgt cgtcgcgcag
      361 ctcgggaata tcgggcactt cgtagaacgg ctcgccgccg tcaccgtggt gccacgccca
      421 gatcatctcg ttgcgctcga ggcacgggaa cgaacgcacc ttcgcctgcg tcggaatgcg
      481 atccatatcg ccgtacggga tctcggtgca tttgccgctc gcaccgtcgt agagccagcc
      541 gtggaacggg caacgaatgc atccaccttc gacgcggcca cccgcgccga ggtgcgcccc
      601 caggtgcgcg cagtaggcct cgaccatgtg cggtgcgccg tcggcggcgc ggaacaacac
      661 gacgtcgcgg ccgaacacgt acatcgcgcg cgtctcgccg ggcatgaggt cacgcgcttc
      721 ggcgacgatg aaccagccgt tcggtaccgg gaacgggaag cgcggctcgg ccgtggccgt
      781 gcgcacgatg ggccggcggc tccggtcgat cagcgcctgc ggatcgcgcg gcatcgggaa
      841 gccggcgaag gtcgcgggtg gcgcggcggg ccggggatcg gtcgtcgaca tgcggcggct
      901 ccgggcgtca ggattccgaa ttgcgttcgg atttaccttc cgaatctagt gttcgggccg
      961 ccggcgcggc aagcatgggc cggcacgccc gcctgacgag aggatcgaca tggccgacga
     1021 gaagtaccgc tacgccgagc gcatgttccc ggcgcccgtc gaccacggca tcgcgcagcc
     1081 cgatcgcgcg gtgacgagcg tgccgttcgg caccagctcg tacctggagg gcggcggtgg
     1141 cgcgggcaac gagtggtacg agggcggcgg cgcgctgaac gagaaggcag tggcggagat
     1201 cgtcgtgtac ccgccgacgc gcggcatcgc aacgcaaggt gatccattcg aaccggtgaa
     1261 gatcggcatc ctcgtcgaca tggacctcgg gcaactgctc gcggactgga tcgacccgac
     1321 gatcctcgcg ctcgaagacg cgctgaacga aggtacgtgg ggtcgttcgc cggtcgcgat
     1381 cgtcacgtgc gacgcgcgtg gcctgccgcg tgagaactac cgaaagctga tcgagggcta
     1441 caagtggctc gtcgagcaag gctgcgtcgt catcctgggt ccgatgatct ccgacaactg
     1501 cttgacgttg cagccgtacg tcgaagagat cggcgtaccg gtgatcggct ggacgggcgc
     1561 gcacaagttc gcgagcgact actgctacac ggtggccaac ggcgacatcc ccaccgaagg
     1621 cgtcatgtgt gcgaattggc tttaccagaa aggcctgaag aaggtcggga tgttctggga
     1681 agtcggctcg tcgggccgtg actacgccga cttcttccgc gacgaagcga ttcgcctcgg
     1741 tctcacgatc acgcgtgaag tgaagctcga gcccaacccg cgcaacttga aggagacgct
     1801 ctccgacatg cgcgacatgg gcacggaggg tctctactac ggcggctacg gctacgcgac
     1861 gttccacttc gccgacgcgc tcaatgccct cgactgggac ccgccgcgcg tcatgggcac
     1921 cgcgttcatg ttctattcga actcgaacaa gtgggccgag ggtctcgaag gctggcacgg
     1981 cgtcgaccag ctcggtgtcg acggcacgaa cccgaactac gaagcgatga tcagtcgctt
     2041 cgagaagcgc ttcggtcgca agacacgcaa cgtcgtcgtc gcactcgcgt acgacacggc
     2101 gcgcgccgcg atccacggca tcgcgaacgc gaagatcccg atcccgaggg aagtgcgcga
     2161 cggtctcgat cgcatccggt ggatgccggc caccaacggc ggaccgtcgt gctacatcca
     2221 gttcgggccc ggcgaccaca agggctacaa gggcgacttc ctcaccatcc gcgagctgcg
     2281 cggcggtgag ctgcgcttcg acggctacta ccgcccggag tggccctcca accggaccga
     2341 ctgaggcgtc ctcctggcgg acccgggcgg gcggctctat tgtcgtacgc gattcggaaa
     2401 ctgagtcagg gggacgcatg gcgcggtatg cggtgatctc ggccgacggg cacgcgggcc
     2461 cgcccgccgc cgtctaccgg gactacctcg atccgggctt ccgggatcgc ttcgacgagc
     2521 accagcgcgc gatggaagag ctgcgcgagg cgatgggccg ttccacgccg tcggagttcc
     2581 aacaggagtg ggaggaggag accggcggcg acggcgggct gaccgcggcc tacgactccg
     2641 acgcgcgcaa cgcggtgctc gacgaagagg gcgtcatcgc cgaagtgctg ttcccggacg
     2701 ccgacgtgct cggcacgggc cgcgtctcgt cgtcaccgtt cggcaccggt ctcgcgggcg
     2761 cgtccgacag cgtcgacgaa gccgtcgcgg gaagccgcgc gcacaaccgc tggctcgcgg
     2821 acttcgtcgg ccaggaaccc gaccgccgca tcggcgtcgc ggtgatcccc gcgatcatcc
     2881 ccgacattga tctcgtgttg cagttggtgc gcgaggccaa ggagctcggc catcgcggca
     2941 tcctcatccc gacgcgttgg ttcgaccgtc ccgcgtatca cgacctgtgt tacgaaccgc
     3001 tgtggtcgtt gaccgaagag ctcggcctcg tgctgcacac gcactcgggt gccggcccga
     3061 ccgacatcgg tctcggtccc ggcatgctgc cgatctacgc atccgaagcg ggctggtggg
     3121 cggcccgccc gctcgcggtg ttgatctggg gtggcatctt cgagcgccac ccggacttga
     3181 ggtactcgat ggccgagaat gccgcgtggt gggtgcccga ccagatccgc aagatggacg
     3241 agaagtggat cggcgggcac aacacgcgca agttcggtga cgcgttccgc cagggcctgt
     3301 cgatgaagcc gagcgactac ctgaatcgca attgcttctt cggtgcgtcg acgcccggtg
     3361 aagacgacat cgaccgccgc cacctcatcg gcatcggcaa catcatgtgg ggcaacgacc
     3421 tgccccatcc cgaaggcacc ttcccgtaca cgaggtactg gatccgcaaa cgcttcaagg
     3481 acgtacccga ggacgagacg cgtcgcatgc tcgggttgac cgcggccgag gtctacgggg
     3541 tcgacctcga cgcgcttgcg ccgctcgtcg agcggatcgg accaacgacc gaagaagtcc
     3601 acggcgacgc accgctcgcg cgcgtgccgg ccggcgtgta gtgcggtagt tcgcatcaag
     3661 gggggacaac atgggacacg ttcgtcggat gggcgcgctc gccgccgtcg ccgcgttgac
     3721 gtttgcggcc ggttgcacga gcgagtccgc gggtgacgac gacgatgccg cgtcgacaca
     3781 gacgacggct gccggcgacg acaccggcgc cgcgacgggc gccggcaaca cggccggcat
     3841 caccgatacg acgatcaaga tctcgttgct gtcgagcgac ctgtcggcgt tgtcggagca
     3901 gaacctcgca cccgagatcg gcagtgcgga cgcgacgatg aaagccgtcg tcgccgacat
     3961 caacgccaac ggtggcgttg cgggccggca gatcgagctc gtgtcgcacg tgatccccgg
     4021 cgccgaggcg atcgtcaacc ccgacgcggg acggcaggcg tgtgtgcagg cgacggagga
     4081 cgaccagccg ttcgcggtga tcctggccgc gtcgctgccc gcgtcgatgg tgcagtgcgt
     4141 cgcgcaggac cacgatccgt tgacgatcac gatggacagc tggcccgaca gtctctacgc
     4201 agcggccgac ggccggttgt tctccgtcgg atcgcagatc tcggtcggga tgagccgtct
     4261 gttcagcgcg tggcccggca tcctcgacga cgccggcgcg ctcgagggca agaccgtcgg
     4321 catcatccgc caggacacgt tatccaacga tcaaaaggat gccgtcgaca actcgttgaa
     4381 gcccgcgctc gaaacgctcg gctacgaggt cgcggccgaa gcggtgttgc cgtgccccga
     4441 gacgtcgcag acctgcgagc aacatcaggc cgcgatccag cgcatgcagg atgccggtgt
     4501 cgacttcgtc tttctcgtcg cgcagaccct ggccggttcg acgactgtcg agaccgcgca
     4561 gagctccggc tacaagccca cgtgggcgac agtcgggaac aatgtgacgc cgacggtcgc
     4621 gaagttctac acgaacgcga gggacaacta cgaaggtgcg tggggtctct cggtcgactt
     4681 caccgacttc accgaggcgg ccgcgaactg caacaggatc gcggtcgcag gtggcgccga
     4741 cgagttcccg acatcctccg acgggtacgg cttcaccggc gtcacgtgct tgcagatgca
     4801 gatcttggcg caggccatcg accgcgtcga cggatcactg gatcaggcgt ccgtgatcgc
     4861 ggcactcgag agtctgggcg atgacgtccc cgcgatctcg ggcggaccga cgtcgctgag
     4921 caaggacaag cacgacgccg ggaacgcggt cttcgtcgcg cgctactccg cggcgacgga
     4981 gaagttcgaa ccggtcgacg atcgcaaacc ggtcgaggtg ccggaatagc gccggggagc
     5041 gagtcggtgc ccgacccgga agcacccgac gccgagcccg aggcaacggc ggcgctcgcg
     5101 gcggcggtgc tcgacgagga gttgcgccga gccgaggccg tcgtccaacc ggacgtcgtg
     5161 ctcgccgaca acgagctgcc gggcgtcgac gacccggcca tgtcgctgcg cgacgcgctg
     5221 cgggtcggcg gcagtcgcac gatcgtcatc gtcggtgtac tgggcgcgct cgcgctcgtc
     5281 gacaacggca tcttcaacgt gctc
//
LOCUS       DASUGA010000494         3571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1404609, whole genome shotgun sequence.
ACCESSION   DASUGA010000494 DASUGA010000000
VERSION     DASUGA010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404609"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VFX21_09720"
     CDS             <1..492
                     /locus_tag="VFX21_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017594869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX5096282.1"
                     /translation="GNDVATGDIGEIWLKGPTVVRGYLGRARETAEAFTDGWFHTGDV
                     GRLDDDGNLYVVDRLKDMIIRGGENIYAAEIEAALYEHADITEVAVIGVPSDRLGEEV
                     GAVVRVRDGAALGEEDVRAHVAGQLAAFKVPAHVWITKDELPRNASGKVLKRELRDQM
                     MSS"
     gene            656..1342
                     /locus_tag="VFX21_09725"
     CDS             656..1342
                     /locus_tag="VFX21_09725"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5096283.1"
                     /translation="MPKTAVLVHGAWHGAWCWELVVPELAARGIDAVTVDLPSVQRPG
                     ASVADDADEVRRAIDAVDGPVVLVGHSYGGAVVTEAGTHPKVQHVVYVTAFALDDGES
                     VSSNALVGGESMRLVEALVFDGEVSTVDPDRATEFFFHDCAPDVAAAAVARLRPMSME
                     AMRTAPRSVAWKDKPATYIVCTDDRGVPVALQRSAAARCAATVEIHTSHSPFLSDPQL
                     LAETLAPFFE"
     gene            1366..1641
                     /locus_tag="VFX21_09730"
     CDS             1366..1641
                     /locus_tag="VFX21_09730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016699530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5096284.1"
                     /translation="MITVRENVEKQRYEISIEDTRVGFAEYRRDGDVLVFPHTEIDSA
                     CRGRGLGAQLVREALDDVRSKGLHVEPRCWFVAQFVDEHPEYRDLVA"
     gene            complement(1648..2346)
                     /locus_tag="VFX21_09735"
     CDS             complement(1648..2346)
                     /locus_tag="VFX21_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5096285.1"
                     /translation="MTTPKAVLLDVGGVLIVPNHDRIMGAFARGGFTPAIDLNRAHYA
                     GAVQLTEERVRDVEWPHYWTSYLDAYITECGVPDELREEVHQHLDSEFSTNELWLQVA
                     DGAHEGLRALAATGVRLGIISNADGTVAEQLRRLEIAQLGAGPGVEMEVLLDSTVVGI
                     AKPDPRIFAMALDEMGLDASDAWYVGDMPGFDVAGARAAGLRPFILDPFQHHLGADYD
                     RVDSLVTLAGMING"
     gene            2396..3571
                     /locus_tag="VFX21_09740"
     CDS             2396..3571
                     /locus_tag="VFX21_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5096286.1"
                     /translation="MEFRRINALPPYAFAQIDALKVQLRRAGEDVVDLAFGNPDMGSP
                     QVAVDKLVEAAQNPRNHRYSTSRGLPKLRLAFADLYQRKFGVSLDFETEVCSTIGVKE
                     GFSHLMLTLVGPGDTARVPSPSYPIHIWGPILAGAGVHYVRMGPDQDFFANLHAAYEQ
                     AWPRPRVIVVSFPHNPTTACVDLEFMTNLVEFARERGIVVVHDFAYADLGFDGYEPPS
                     ILQVPGAKEVAVELYSMTKSFAMAGWRMGFVAGNTEIVAALGKLKSYLDYGSFQPIQI
                     AATVAMNEAPDFPLEMNDIYKSRRDTLIDGLDRIGWHVERPKGTMFVWAPIPEPYAEM
                     SSIEFATMCVQEAKVAVSPGNGFGPGGEGFIRFALVENDKRIQQAIRQFRKALTKLG"
BASE COUNT          601 a         1191 c         1211 g          568 t
ORIGIN      
        1 ggcaacgacg tcgcgaccgg cgacatcggc gagatctggc tgaagggccc gacggtcgtg
       61 cgcggctact tgggtcgggc gcgggagacc gcagaggcgt tcaccgatgg ctggttccac
      121 accggcgacg tcggccggct cgacgacgac ggcaacctct acgtcgtcga ccggctgaag
      181 gacatgatca tccgtggcgg cgagaacatc tacgcggccg agatcgaggc cgccctctac
      241 gagcacgccg acatcaccga ggtcgcggtc atcggcgtcc cgagcgaccg actcggcgag
      301 gaggtcgggg cggtcgtgcg cgtccgagac ggtgcggcgc tcggcgaaga agacgtgcgc
      361 gcgcacgtgg ccgggcagct cgcggcgttc aaggtgccgg cgcacgtgtg gatcaccaag
      421 gacgagctcc cgcgcaatgc gagcgggaag gtgctgaagc gggagttgcg cgaccagatg
      481 atgagctcgt gagcgactac atcgagatcg cgtacgacgt ccacggccac gtcgccgtcg
      541 tcaccatcga ccggcccgag gcacgacccg aacattgtcg atcccgcccg ctcacagcgg
      601 gctggattga ccgagaacca ggttggtgcg agcaagacgg gtacgttcac gagcgatgcc
      661 caagaccgca gtgctcgtcc acggcgcctg gcacggcgcg tggtgctggg aactggtcgt
      721 gccggagctc gccgcacgag gcatcgatgc ggtgacggtc gacctgccgt ctgtgcagcg
      781 ccccggcgcg tcggtggccg acgacgctga cgaagtccgg cgcgcgatcg acgcagtcga
      841 cggacccgtc gtgctcgtcg gccactcgta cggcggggcc gtggtcacgg aggccggcac
      901 gcatcccaag gtgcaacatg tcgtctacgt cactgcgttc gcactcgacg acggtgagag
      961 cgtgagcagc aatgcgctcg tgggcggaga gtcgatgcgg ctcgtcgaag cactcgtgtt
     1021 cgacggcgaa gtctcgaccg tcgacccgga ccgcgcgacc gagttcttct ttcacgactg
     1081 cgcgcccgat gtcgccgcgg ccgcggtcgc gcggctacgg ccgatgtcga tggaagcgat
     1141 gcggacggcg ccccgttcgg tcgcatggaa ggacaagccc gcgacctaca tcgtgtgcac
     1201 cgacgatcgt ggtgtcccag ttgcgttgca gcgatccgcg gcggcccgct gcgctgcgac
     1261 agtcgagatc cacaccagcc actcgccgtt cctcagcgat ccgcagctgc tcgccgagac
     1321 cctcgcaccg tttttcgaat gacgcatacc gggaagactc aacccgtgat cacggtccgg
     1381 gagaacgtgg agaagcagcg gtacgaaatc tccatcgagg acacgcgggt cggattcgcg
     1441 gaataccggc gcgacggcga cgtactcgtc tttccgcaca ccgagatcga ttcggcctgc
     1501 cgcggacgcg gtctcggcgc gcagctcgta cgcgaagcgc tcgatgatgt gcggtcgaag
     1561 ggactgcacg tcgaacctcg ctgttggttc gtcgcgcagt tcgtcgacga gcaccccgaa
     1621 tatcgcgacc tcgtcgcgta gctcgcgtca gccgttgatc attccggcga gcgtgaccag
     1681 cgagtcgacg cggtcgtagt cggcaccgag gtggtgctgg aacgggtcga ggatgaacgg
     1741 ccgcaacccg gccgcgcgcg caccggcgac gtcgaagccg ggcatgtcgc cgacgtacca
     1801 cgcgtcgctc gcgtcgaggc ccatctcgtc gagcgccatg gcgaagatgc gcgggtcggg
     1861 cttggcgatc ccgacaaccg tcgagtcgag cagcacttcc atctcgacgc ccgggcccgc
     1921 gccgagctgc gcgatttcga gccgccgcaa ctgctcggcg acagtgccgt ccgcgttcga
     1981 gatgatgccg agccgcacgc cggtcgcggc gagcgcgcga aggccttcgt gcgcgccgtc
     2041 ggcaacctgc agccacagct cgttcgtgga gaactcactg tcgaggtgct ggtgtacctc
     2101 ctcgcgcagc tcgtcgggga caccgcactc cgtgatgtac gcgtccagat acgacgtcca
     2161 gtagtgcggc cactcgacat cgcgcacgcg ctcctcggtc agctgtaccg cgccggcgta
     2221 gtgcgcacgg ttgaggtcga tggcgggcgt gaagccgccg cgggcgaacg cgcccatgat
     2281 ccggtcgtgg ttcggcacga tgaggacgcc gcccacgtcg agcagcacgg ccttcggagt
     2341 cgtcactttc gaaaagctac cgaagggctt ccggaccgcc ttgtatcctg gccctatgga
     2401 atttcgccgg atcaacgccc ttcctccgta tgcgttcgcc cagatcgacg cattgaaggt
     2461 gcaactccgg cgagcaggcg aggacgtcgt cgacctcgcg ttcggcaacc ccgacatggg
     2521 gtcgccgcag gtcgcagtgg acaagctcgt cgaggccgcg cagaacccgc gcaaccaccg
     2581 ttactcgacg agccgcgggt tgccgaagct gcggctcgcg ttcgccgacc tctaccagcg
     2641 caagttcggc gtgagcctcg acttcgagac ggaggtgtgc tcgacgatcg gcgtcaagga
     2701 gggtttctcg cacttgatgc tgacgctcgt cggcccgggt gacaccgcgc gggtgccgag
     2761 cccgtcgtac ccgatccaca tctgggggcc gatcctcgcg ggcgcgggcg tccactacgt
     2821 gcgcatgggt cccgaccaag acttcttcgc gaacttgcat gccgcgtacg aacaggcgtg
     2881 gcctcggccg cgcgttatcg tcgtgtcgtt cccgcacaac ccgacgaccg cgtgcgtcga
     2941 tctcgagttc atgacgaacc tcgtcgagtt cgcccgcgag cgcggcatcg tcgtcgtgca
     3001 cgacttcgcg tacgcggatc tcggcttcga cggctacgag ccgccgtcga tcctccaggt
     3061 gcccggtgcg aaggaggtcg cggtcgagct gtactcgatg acgaagtcgt tcgcgatggc
     3121 cggctggcgc atgggcttcg tcgccggcaa caccgagatc gtcgccgcgc tcgggaagct
     3181 gaagtcgtac ctcgactacg ggtcgttcca gcccatccag atcgcggcga cggtcgcgat
     3241 gaacgaagcg cccgacttcc cgctcgagat gaacgacatc tacaagtcgc gccgcgacac
     3301 gttgatcgac ggcctcgacc gcatcggctg gcatgtcgaa cgccccaagg ggacgatgtt
     3361 cgtgtgggcg ccgatccccg agccctacgc cgagatgtcg agcatcgagt tcgcgacgat
     3421 gtgcgtgcag gaagcgaagg tcgcggtgtc acccggcaac ggcttcggcc ccggcggtga
     3481 gggcttcatc cgcttcgcgc tcgtcgagaa cgacaagcgc atccagcaag cgatccgcca
     3541 gttccgcaaa gcgctgacca agctcggcta g
//
LOCUS       DASUGA010000495         2095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_780550, whole genome shotgun sequence.
ACCESSION   DASUGA010000495 DASUGA010000000
VERSION     DASUGA010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780550"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..503)
                     /locus_tag="VFX21_09745"
     CDS             complement(24..503)
                     /locus_tag="VFX21_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5096287.1"
                     /translation="MITGLAHTALHVPDVEAAVAWYRDVLGLRVLSPPFLMEGDAISA
                     DMGELLPSPVAVKASIVGAGDDTDQVIEVVEYPNVPSGAARADIARAGYTHVGLVCDD
                     IDATRTALEAQGVTFLTQRIADVAGLRTTWFADPWGNVFILMQKVRRPGRPYYRQFG"
     gene            complement(500..1294)
                     /locus_tag="VFX21_09750"
     CDS             complement(500..1294)
                     /locus_tag="VFX21_09750"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5096288.1"
                     /translation="MEPKGTALVTGASRGIGRAVAQELAARGFDTIATMRNLDDGAGL
                     DNCTVQRLDVDDPASYVIPDGLKVVVNNAGVESDNLPLELMPDAMWRKLFETNVFGLV
                     SVTKAAIPVLRNAGGGVICNVTSSSLLAPVPFLGAYRASKAAVSAICESLRTEVAPFG
                     IRVVEIMPGPIETDMLLTSERPSPAIDDEHYHDLAARMWSTREHVKALYTPAPEAARR
                     IVDAICDDDGPMRYGCDDMSEGMLTAWRGAESDEVWMRSMGSAFNP"
     gene            1330..>2095
                     /locus_tag="VFX21_09755"
     CDS             1330..>2095
                     /locus_tag="VFX21_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5096289.1"
                     /translation="MDLRFTPEQEAFRADARAWLTERLEGPYASVRGRGGPGDEHELF
                     DERWEWEQELGRGNWIGLGWPSEYGGRGVTLLEQVIFFEEYARAGGPGRVGIVSEGLL
                     GPTIVHFGNDEQKRRFLPGILQGTEIWCQGYSEPNAGSDLANVQTRAELDGGEWVVHG
                     QKVWTSLAHWAQWIFVLCRTDRDAPKHKGLSYLLMPMDQTGIEIRPIVQITGHSEFNE
                     TFFDGARTPVDQVVGEVNGGWRVAMGTLAFERGASTL"
BASE COUNT          321 a          707 c          734 g          333 t
ORIGIN      
        1 gtccagattc gagtaggtgt cgctcatccg aactggcggt agtaggggcg gccgggccgg
       61 cggaccttct gcatgaggat gaacacgttg ccccacggat cggcgaacca ggtcgtgcgg
      121 agccccgcaa cgtcggcgat gcgttgcgtg aggaacgtca cgccctgcgc ttcgagcgcg
      181 gtgcgcgtcg cgtcgatgtc gtcgcagacg agcccgacgt gcgtgtaccc ggcgcgcgcg
      241 atgtcggcgc gggctgcgcc gctgggcacg ttcgggtact cgacgacctc gatgacctgg
      301 tcggtgtcgt cgccggcgcc gacgatggac gccttcaccg cgacgggtga cggcaacagc
      361 tcgcccatgt ccgcgctgat cgcgtcgccc tccatgagga acggcggcga gaggacgcgc
      421 aggccgagca cgtctcgata ccacgcaacg gccgcctcga catcgggtac gtgcaacgcg
      481 gtgtgcgcga gaccggtgat cacgggttga acgcgctgcc catcgagcgc atccacactt
      541 cgtcgctctc ggcgccgcgc cacgccgtga gcatgccttc gctcatgtcg tcgcagccgt
      601 agcgcatcgg tccgtcgtcg tcgcatatcg cgtcgacgat gcggcgggcg gcctcgggcg
      661 cgggcgtgta caacgccttg acatgctcac gcgtcgacca catgcgcgcc gcgaggtcgt
      721 ggtagtgctc gtcgtcgatc gccggtgacg gtcgctcgga cgtcagcagc atgtcggtct
      781 cgatcgggcc cggcatgatc tcgacgaccc ggatgccgaa cggcgcgacc tccgtgcgca
      841 gtgactcaca gatcgcggac accgcggcct tgctcgcccg gtatgccccg aggaacggca
      901 ccggcgccag cagcgacgag ctggtcacgt tgcagatgac gccgccaccg gcgttgcgca
      961 gcaccgggat cgcggccttc gtcaccgaca cgaggccgaa cacgttcgtc tcgaacaact
     1021 tgcgccacat ggcgtccggc atcagctcga gcggcagatt gtcgctctcg acgccggcgt
     1081 tgttgacgac aaccttgagc ccgtccggga tcacgtacga cgccgggtcg tcgacgtcga
     1141 gccgctgcac cgtgcagttg tcgagccccg cgccgtcgtc gagattgcgc atcgtcgcga
     1201 tcgtgtcgaa gccgcgcgct gcaagctctt gcgcgaccgc gcggccgatc ccccggctcg
     1261 cgcccgtgac cagtgcggtt cccttcggct ccactgcccg gactctaacg aggtactttg
     1321 ccgagcgttg tggacctccg attcacgccc gagcaagaag cgtttcgtgc cgatgcgcgt
     1381 gcgtggctga ccgaacggct cgagggcccc tatgcgtcgg tacgcggccg cggcggtccg
     1441 ggcgacgagc acgagctgtt cgacgagcgc tgggagtggg agcaagagct cggccgcggc
     1501 aactggatcg gcctcggctg gccgtccgag tacggcggcc gcggcgtgac gctgctcgag
     1561 caagtcatct tcttcgagga gtacgcgcgc gccggtgggc cggggcgggt cggcatcgtc
     1621 agcgagggcc tgctcggccc gacgatcgtg catttcggca acgacgagca gaagcgccgc
     1681 ttcctgccgg gcatcctgca gggcaccgag atctggtgcc agggctactc cgagccgaac
     1741 gcgggctccg atctcgcgaa cgtgcagacg cgcgccgagc tcgacggcgg cgagtgggtc
     1801 gtgcacggcc agaaggtgtg gacgtcgctc gcgcactggg cgcagtggat cttcgtgctg
     1861 tgccgcaccg accgcgacgc accgaagcac aaaggactgt cgtacttgtt gatgccgatg
     1921 gaccaaaccg gcatcgagat ccgtccgatc gtgcagatca ccgggcacag cgagttcaac
     1981 gagacgttct tcgacggcgc ccgcacaccg gtcgaccagg tcgtcggcga ggtcaacggc
     2041 ggctggcggg tcgcgatggg cacactcgcg ttcgagcggg gcgcgtcgac gctcg
//
LOCUS       DASUGA010000496         2396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_93997, whole genome shotgun sequence.
ACCESSION   DASUGA010000496 DASUGA010000000
VERSION     DASUGA010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93997"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VFX21_09760"
     CDS             complement(<1..908)
                     /locus_tag="VFX21_09760"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX5096290.1"
                     /translation="MTQRLSFVCNGDDVIVDVEAGESLLSVLRERLRLTSVKDGCAPQ
                     GQCGCCTVLVDGDARVACVTPATRVAGRSVTTVEGLDESMRAQFADAFVVSGGSQCGF
                     CTPGTVMRFATKQTRNVDRGLAAHLCRCTGWLTVKDALAGVATGSSHTDAASRRAELE
                     GRWPQIVGPSTPLGGAPFADDTAPAGALVAVPLPPDSTATAVDAAGARWVVADSLLEA
                     RALAAKVQGRRTTMQSRPPLLGAMPERPDGGVALATSWVEPAYLEPDASWCVPGGEPA
                     SPLANGGAFGGKEASLAPRAARELAD"
     gene            complement(918..2177)
                     /locus_tag="VFX21_09765"
     CDS             complement(918..2177)
                     /locus_tag="VFX21_09765"
                     /inference="COORDINATES: protein motif:HMM:NF040341.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6174 domain-containing protein"
                     /protein_id="HEX5096291.1"
                     /translation="MSERLERAGRAVVDAPVSLPLETVELERRVSRRRTRRAASIVGA
                     VALVALVAASVVVLNGGDDDATQPIITDGETTVPTSVPTVSGDEIGVLVRDGQVQEGK
                     VVDLDGDIIGAVALPDDLSVGGQIFLRTDADGGYELDPRGFRGTYQDCTYAGDLASDT
                     EFRVCADHISEIEDDGSERQIIGTPPNATGGRWDTALVSPDGSTLLASILGECDSPTT
                     YFVRVDGTDTKAVTGPDLASAPVGWISAESAVIASSGRCGRPAGGPEGAVVVTPSGIP
                     NVIYEFEHPDDAQVFVWTRLDDDGTAALQQARSAWDALDAHDYSFDFQARCFCPVWPT
                     HVTVVDDKVASATLTVEGRAVSTDLGETVPQLLDRILASELNRAAGLVGARYDERGVP
                     VEASIDTIRNAVDDEYAWTISNLVIDT"
     gene            complement(2174..>2396)
                     /locus_tag="VFX21_09770"
     CDS             complement(2174..>2396)
                     /locus_tag="VFX21_09770"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX5096292.1"
                     /translation="DVVPATDPELWAAVRTLPPRQCQAIALRFVADLPEAEIASVMGV
                     ARGTVASTLADARARLAQLLAEPALMEDR"
BASE COUNT          381 a          853 c          807 g          355 t
ORIGIN      
        1 tgatcggcca gctcgcgcgc cgcgcgcggc gcaagcgacg cttccttgcc accgaacgca
       61 ccaccgttgg cgagcggcga cgcgggttcg ccgccgggta cgcaccacga cgcatcgggt
      121 tccagatacg cgggctcgac ccacgacgtc gcgagcgcga cgccgccgtc cggacgctcc
      181 ggcatcgcgc cgagcagcgg cggccgcgac tgcatcgtcg tccggcgtcc ctgcaccttc
      241 gcggcgaggg cgcgcgcttc gagcaacgag tcggccacga cccatcgcgc gcccgcggca
      301 tcgaccgcgg tcgcggtgga gtccggtggg agcgggaccg cgacgagcgc acccgcgggt
      361 gccgtgtcgt cggcgaacgg cgcgccaccg agcggcgtcg acgggccgac gatctgcggc
      421 catctacctt ccagttccgc gcgccgcgac gcggcgtcag tatgcgacga accggtcgcg
      481 acaccggcga gcgcatcctt caccgtcagc cagcccgtgc agcggcacag gtgcgcggca
      541 aggccacgat cgacgttgcg cgtctgcttc gtcgcgaacc gcatcacggt gccgggcgtg
      601 cagaacccgc actgcgatcc gcctgataca acgaacgcgt cggcaaactg cgcacgcatc
      661 gactcgtcga ggccttcgac ggtcgtgacg ctccgacccg cgacacgagt cgccggtgtg
      721 acgcacgcga cgcgcgcgtc cccgtcgacc agcacggtgc agcatccaca ctgtccttgc
      781 ggcgcgcagc cgtccttcac cgacgtcaac cgcagccgtt cgcgcaacac cgagagcaat
      841 gattcgccgg cctcgacgtc gacgatgacg tcgtcaccgt tgcatacgaa cgagagtcgt
      901 tgcgtcaccc ccgcaggcta cgtgtcgatc acgaggttcg agatcgtcca ggcgtactcg
      961 tcgtcgacgg catttcggat cgtgtcgatc gacgcctcta cgggaacacc gcgctcgtcg
     1021 tagcgcgcgc cgaccaagcc cgcggcgcgg ttcagctcgg atgcgaggat tcggtcgagc
     1081 agctgcggga ctgtctcgcc cagatccgtc gacaccgcgc gtccttcgac cgttagtgtc
     1141 gccgacgcaa ccttgtcgtc aacgacagtg acgtgcgtcg gccagaccgg acagaaacaa
     1201 cgggcctgga aatcgaacga gtagtcgtgc gcgtcgagcg cgtcccaggc ggagcgagcc
     1261 tgctgcagcg cagccgttcc atcgtcgtcg agacgcgtcc acacgaagac ctgagcgtcg
     1321 tcggggtgct cgaactcgta gatcacgttc gggatgcccg agggagtgac cacgaccgct
     1381 ccctcgggac cgcccgcggg ccgaccgcaa cgcccggacg acgcgatcac cgcggactcg
     1441 gcgctgatcc acccaaccgg cgcgctcgcg aggtcgggtc cggtgaccgc tttggtatca
     1501 gtcccgtcga cgcgcacgaa gtacgtcgtc ggcgaatcac attcgccgag gatcgacgcg
     1561 agcaaagtcg agccgtcggg cgagaccaac gcggtgtccc agcgacctcc cgtcgcgttg
     1621 ggtggtgtcc cgatgatctg gcgttcactg ccgtcgtcct cgatctcgct gatgtggtcc
     1681 gcgcacacgc ggaactctgt gtccgacgca agatcgccgg cgtacgtgca gtcctggtag
     1741 gtaccccgga acccgcgtgg gtcgagctca taaccgccgt cggcatcggt gcgcaggaag
     1801 atctgcccgc ccaccgaaag atcgtcgggc agagcgaccg ctccgatgat gtcgccgtcg
     1861 aggtcgacga ccttgccctc ttgaacttgt ccgtcgcgga cgagcacgcc gatctcgtcg
     1921 cccgacaccg ttggaacact cgtcggaacg gtcgtctctc cgtcggtgat gatcggttgg
     1981 gttgcatcgt cgtcgccacc gttgagcacg acgaccgatg ccgcgacgag cgcgacgagc
     2041 gcgacagcgc cgacgatgct cgcggcgcgg cgggtcctgc ggcgcgacac gcgccgttcc
     2101 agctcgacgg tctcgagcgg caacgacacc ggtgcgtcga cgaccgcgcg tccggcgcgt
     2161 tccaagcgtt cgctcatcgg tcctccatca gcgcgggctc cgcgaggagt tgcgcgagcc
     2221 gggcacgcgc gtcggcgagc gtcgacgcga cggtgccacg cgcgacgccc atgacggatg
     2281 cgatctcggc ttcgggtagg tcggcgacga agcgcagcgc gatcgcctgg cattgccgcg
     2341 gcggcagcgt gcgtactgcc gcccagagtt cggggtcggt cgcggggacg acgtcg
//
LOCUS       DASUGA010000497         3244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1300513, whole genome shotgun sequence.
ACCESSION   DASUGA010000497 DASUGA010000000
VERSION     DASUGA010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300513"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VFX21_09775"
     CDS             <1..548
                     /locus_tag="VFX21_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5096293.1"
                     /translation="HIAAGAGHDITYVESELYGAVDALGRERFDLVYTGVGALGWLPD
                     VKQWAKVVAALLRPGGRLYIREGHPMLWTLDETRDDELLVAGYAYFETAEPMEFFSEE
                     TYTDGDEKVGSPQTREWNHGLGEIVQAVIEAGLAVTRVAEHQFCEWLALPWMVERDGR
                     YYLPDHPERLPLMYTLEACKA"
     gene            884..1528
                     /gene="rplC"
                     /locus_tag="VFX21_09780"
     CDS             884..1528
                     /gene="rplC"
                     /locus_tag="VFX21_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX5096294.1"
                     /translation="MASKGILGRKLGMTQVFDAENRIVPVTVIEAGPCRVTQLRTPER
                     DGYSAVQLSFGDTKAHRLSKAELGHLKGADAPPQKYLVELRVDSLDGFEIGATLKADV
                     FEAGEKVDVTGISKGHGFSGAMKRHNFSGQGASHGNHKKHRAPGAVGACATPARVFKG
                     TRMAGQYGGTRVTTLNLEVVEGDAERGILLIKGSVPGPNGGLVFVRNATKVAAK"
     gene            1540..2196
                     /gene="rplD"
                     /locus_tag="VFX21_09785"
     CDS             1540..2196
                     /gene="rplD"
                     /locus_tag="VFX21_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX5096295.1"
                     /translation="MKTAAGGDGASVELPDDIFGIEPNVAVLHQVVTAQLAAKRAGTH
                     NTKTRAEVSGGGAKPWRQKGTGRARQGSIRSPQWRGGGVAHGPKPRDYSQRTPKKMIR
                     LALKSALSDRANESKVMVVDDWNIAAPKTKQAIERLGALGLRTKGEWEPRVLIVVQPT
                     EEAVWKSFRNLDGRVQLIVPNEMNAYDILVNDWLVFSHASLDNVVARLSGNAPEDDSE
                     "
     gene            2189..2485
                     /gene="rplW"
                     /locus_tag="VFX21_09790"
     CDS             2189..2485
                     /gene="rplW"
                     /locus_tag="VFX21_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX5096296.1"
                     /translation="MNKDPRDIIIRPVVSEKSYAAYDLNVYTFIVNPDANKIEIRKAV
                     EELFGKKVEKVATINRKGKRKRNRRTGTWSNRVSQKRAIVTLAAGEDRIDIFGS"
     gene            2488..>3244
                     /gene="rplB"
                     /locus_tag="VFX21_09795"
     CDS             2488..>3244
                     /gene="rplB"
                     /locus_tag="VFX21_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX5096297.1"
                     /translation="MPIRKRKPTSPGRRFQTSSDFSEVTKDRPEKRLTKPKPKSGGRN
                     GYGRMTARHRGGGHKQRYRVVDFKRNKDGVPAKVAAIEYDPNRNARIALLHYRDGEKR
                     YILAPQGLSVGDLLQSGQGSEIRIGNALPLRYIPVGTTVHNVELKPGGGGKMGRGAGA
                     AIQLIAKEGAFATLRLPSTEMRRVPIDCRATVGTVGNSEAGLISIGKAGRNRWKGKRP
                     HTRGVAMNPVDHPLGGGEGKSSGGRHPVSPWGKP"
BASE COUNT          596 a         1110 c         1101 g          437 t
ORIGIN      
        1 ggcacatcgc ggcgggcgcg ggtcacgaca tcacctacgt ggagtccgag ctgtacggag
       61 cagtcgacgc gctcggtcgg gaacgcttcg acctcgtgta cacgggcgtc ggcgcgctcg
      121 gctggcttcc cgacgtgaag cagtgggcga aggtcgtcgc cgcgctcctg cgccccggag
      181 gtcggctcta catccgtgag ggccacccga tgctgtggac cctcgacgag acccgcgacg
      241 acgagctcct cgtcgccggg tacgcgtact tcgagacggc cgagcccatg gagttcttca
      301 gcgaggagac ctacaccgac ggcgacgaga aggtcggctc accacagacg cgggagtgga
      361 atcacggcct cggcgagatc gtccaggcag tcatcgaggc cggcctggcc gtcacgcggg
      421 tcgccgagca ccagttctgc gagtggctgg ccctcccctg gatggtcgaa cgcgacggcc
      481 gctactacct ccccgaccac ccggaacggt tgccgctcat gtacaccctc gaagcctgca
      541 aagcctgagt agcgagaacc ggcaagaaac gcgtctccga gggcacttgc gatccgtggt
      601 cacgaattcg gcagatactc gccgaaatcg tgaccacggc gggccaggat cggcccgggt
      661 agcggggccc ggggcggggc gctaatattt cctggctctg cggactccgg tcttccggcc
      721 gttcgagttc gcgtcaagtc cagaaggccc accagcaacc gaggcccgcg caggccccat
      781 tttccgggga accgcgcggc acaaccgaga ccagcgctcc gccggggttc tttgcccgag
      841 cggagcgttc gcgtgactac cccacacgag ggacgacaga ccgatggcat ctaaagggat
      901 cctgggccgc aagctcggca tgacccaggt cttcgacgcc gagaaccgga tcgtgccggt
      961 gacggtgatc gaagcggggc cgtgccgcgt gacccagctg cgcacgccgg agcgcgacgg
     1021 ctacagcgcg gtgcagctgt cgttcggcga cacgaaggcc caccgcctgt cgaaggccga
     1081 gctcggtcac ctcaagggtg ccgacgcgcc gccgcagaag tacctcgtcg aactgcgcgt
     1141 cgactcgctc gacggcttcg agatcggcgc cacgctcaag gccgacgtct tcgaagcggg
     1201 cgagaaggtc gacgtcaccg gcatctcgaa gggccacggc ttctcgggcg cgatgaagcg
     1261 ccacaacttc tccggccagg gcgcgtcgca cggtaaccac aagaagcacc gtgcgcccgg
     1321 tgcggtcggc gcgtgcgcca cgcccgcgcg cgtgttcaag ggcacgcgca tggcgggcca
     1381 atacggcggc acgcgcgtca cgacactcaa cctcgaagtc gtcgaaggcg acgccgagcg
     1441 cggcatcttg ctcatcaagg gttcggtgcc gggccccaac ggcggcctcg tgttcgtccg
     1501 caacgcgacg aaggtggcgg ccaagtgagc accatcacga tgaagaccgc ggccggcggc
     1561 gacggcgcga gcgtcgagct ccccgacgac atcttcggca tcgagcccaa cgtcgcggtg
     1621 ttgcaccagg tcgtcaccgc ccagctggcg gccaagcgag ccggcacgca caacacgaag
     1681 acccgcgccg aggtgtccgg tgggggcgcc aagccgtggc ggcagaaggg caccggccgc
     1741 gcccgccagg gctcgatccg ttcgccgcag tggcgcggtg gtggtgtggc gcacggtccc
     1801 aagccgcgcg actactcgca gcgcacaccg aagaagatga tccgcctcgc gctgaagtcc
     1861 gcgctctccg accgtgcgaa cgagtcgaag gtcatggtcg tcgacgactg gaacatcgcc
     1921 gcgccgaaga cgaagcaagc gatcgagcgg ctcggtgcgc tcggcctgcg caccaagggc
     1981 gagtgggaac cgcgtgtgct catcgtcgtg cagccgaccg aagaggcggt gtggaagtcg
     2041 ttccgcaacc tcgacggtcg cgtgcagctc atcgtcccca acgagatgaa cgcctacgac
     2101 atcttggtga acgactggct ggtgttctcg cacgcgtcgc tcgacaacgt cgtcgcccgt
     2161 ctgagcggca acgcaccgga ggacgacagt gaataaggac ccgcgcgaca tcatcattcg
     2221 tccggtcgtg tcggagaagt cgtacgcggc ctacgacctg aacgtctaca cgttcatcgt
     2281 gaacccggac gccaacaaga tcgagatccg caaggccgtc gaggagcttt tcggcaagaa
     2341 ggtcgagaag gtcgcgacga tcaaccgcaa gggcaagcgc aagcgcaacc gccggaccgg
     2401 cacgtggtcg aaccgggttt cgcaaaagcg cgccatcgtc accctcgccg ccggcgagga
     2461 ccgcatcgac atcttcggga gctgacggtg ccgatccgta agcgcaagcc cacgagcccc
     2521 ggacgtcgtt tccagacgtc gtccgacttc tccgaggtca ccaaggaccg tcccgagaag
     2581 cggctcacca agcccaagcc caagagcggt ggccgcaacg gctacggccg gatgaccgcg
     2641 cgccaccgcg gcggtggtca caagcagcgc taccgcgtcg tcgacttcaa gcggaacaag
     2701 gacggcgtcc ccgcgaaggt cgcggcgatc gagtacgacc cgaaccgcaa tgcgcgcatc
     2761 gcgctgttgc actaccgcga cggcgagaag cgatacatcc tcgcgccgca gggcctcagc
     2821 gtcggcgacc tgctgcagag cggtcagggg tcggagatcc gcatcggcaa cgcgttgccg
     2881 ttgcgctaca tcccggtcgg cacgaccgtg cacaacgtcg agctgaagcc gggcggcggc
     2941 ggcaagatgg gccgcggtgc cggtgccgcg atccagctca tcgcgaagga aggcgcgttc
     3001 gcgacgctgc ggctcccgtc caccgagatg cgccgcgtgc cgatcgactg ccgcgcgacc
     3061 gtcggcaccg tcggcaacag cgaggcgggc ctgatctcga tcggcaaggc cggtcggaac
     3121 cggtggaagg gcaagcgccc gcacacccgt ggtgtcgcca tgaaccccgt cgaccacccg
     3181 ctcggcggtg gcgaaggcaa gagctcgggt ggtcgccacc cggtgtcgcc gtggggcaag
     3241 cccg
//
LOCUS       DASUGA010000498         2599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_609605, whole genome shotgun sequence.
ACCESSION   DASUGA010000498 DASUGA010000000
VERSION     DASUGA010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609605"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2337
                     /locus_tag="VFX21_09800"
     CDS             <1..2337
                     /locus_tag="VFX21_09800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX5096298.1"
                     /translation="SGGAFHYFEPVQALDGYDVVETVAAQPWVAHGKPGMVGISYPGI
                     AQTFVAPTQPPHLAAIAPLSVYADTYRSLAEPGGIPNIGFPREWAAERDNNAKPYGQG
                     WEQGIVDAGGPNGAQCAANQLQRGDNVSLVEAFDDHPYREPVDLADGLSPELLAPQIN
                     VPVFMGGAWQDEQTGGSSSSLFPHLTGVPAGKLHLFQTNGTHVDSLVAELNRWYEFLE
                     FYVAQRIPEVPGSIRALAPAIFQVATGISGVQLEADRFTSYPNYAAALAAYEAEAPVR
                     ILFENGAGDTTNLGAPFGTTEMRFASWPPPNAVPTRWYFQPDQQLGSSAPGIADGDGK
                     AATSYVYDPAAKPATSFHGSTSDIWNAHPAFDWRVLPVGKALAFDSAPLAATTTVAGP
                     GSLDLWLRSTAADTDIEVTVSELRPDGEEMFVQNGWLRASHRALTNDSTELEPKHPHT
                     SAAAAELPAGEFVSARVALFPFAHIFRAGSQLRITIEAPGGNRPFWAFKDLPANGVVV
                     NEIAHSVGHRSSVVLPVVPNPTPTPAVRPPCGSTRGQPCRPIASKAIATNVTAHQNGG
                     DGAIVTWTAPVPRDDDATATGIAAHQVVAQPGGATVTVDPNQTLATFVDMPDGTYSFT
                     VYAGGVAASTPSQSITITDVPTTTSTTSTTSTTSTTTSTTSTSTSTTSTTTSTTTTVP
                     VTTTTVPDTTSTTLAPSATTSTTVAVGTEGTTVPPVVEGNDIDSGGGESGGGSLPRTG
                     ASLALLVLLGTAFVVVGLIARRRSPEAAHTASSRLRGR"
     gene            2334..>2599
                     /locus_tag="VFX21_09805"
     CDS             2334..>2599
                     /locus_tag="VFX21_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5096299.1"
                     /translation="MTDVAAAPAKERWSFSWKHLYDEVVTSGLCTGCAGCVIACPHDV
                     LGYDDTNGVYKPQQIEDYGGPSDCSHGDKACTSCTRACPRFRAW"
BASE COUNT          444 a          935 c          847 g          373 t
ORIGIN      
        1 agcggcggcg cgttccacta cttcgaaccc gtgcaggccc tcgacggtta cgacgtcgtc
       61 gagaccgttg ccgcgcaacc gtgggtcgcg cacggcaagc cgggcatggt tggcatctcg
      121 tacccgggca tcgcgcaaac cttcgtcgcg ccgacccagc ccccgcacct ggccgcgatc
      181 gcgccgctgt cggtctatgc cgacacgtac cgcagcctcg cggaacccgg tggcattccg
      241 aacatcgggt tcccccgaga gtgggcggcc gaacgggaca acaacgcgaa gccgtacggc
      301 caaggttggg agcaaggcat cgtcgacgcg ggtggtccga acggtgcgca gtgtgcagcg
      361 aaccaactgc aacgcggcga caacgtcagc ctcgtcgagg cattcgacga ccacccctac
      421 cgcgagccgg tcgatctcgc cgacggcctg tcgccagaac tgctcgcgcc gcagatcaac
      481 gtgccggtgt tcatgggcgg cgcctggcag gacgagcaga ccggcggttc gtcgtcgtcg
      541 ctgttcccgc acctgaccgg cgttccggcg ggcaagctgc atctgttcca gacgaacggc
      601 acgcacgtcg acagcctcgt cgccgagctc aaccgctggt acgagttcct cgagttctac
      661 gtggcgcagc gcattccgga ggtgcccgga agcatccgcg cgctcgcgcc ggcgatcttc
      721 caggtcgcga ccggtatcag cggcgtgcag ctcgaagccg accggttcac gagctatccg
      781 aactacgccg cggccctggc cgcctacgaa gccgaggcgc cggtccgcat cctgttcgag
      841 aacggtgccg gcgacacgac caatctcggc gcgccattcg ggacgaccga aatgcggttc
      901 gcgtcgtggc cacccccgaa tgccgtcccg acgcgctggt acttccagcc cgaccagcag
      961 ttgggatcgt ccgcgcccgg catcgccgac ggcgacggca aagcggcgac gtcgtacgtg
     1021 tacgacccgg ccgccaagcc cgcgacgagc ttccacggca gcacctccga catctggaat
     1081 gcccacccgg cgttcgactg gcgcgtgctg ccggtcggga aggcgctggc gttcgacagc
     1141 gcaccgctgg cggcgaccac gacggtggcg gggcccggca gtctcgacct ctggctgcgc
     1201 tcgacggctg ccgacaccga catcgaagtc acggtctcgg aactccggcc cgacggcgaa
     1261 gagatgttcg tgcaaaacgg atggctgcga gcttcgcatc gcgcgctcac gaatgattcg
     1321 acggagctcg aaccgaagca tccgcacacg tcggcagccg cggccgaact tccggccggc
     1381 gaattcgtca gcgcgcgggt tgccctgttc ccattcgcgc acatcttccg ggccggatcg
     1441 caactgcgca tcacgatcga agcgcccggc ggcaaccggc cgttctgggc gttcaaggat
     1501 cttcctgcga acggcgtggt cgtgaacgag atcgcgcatt ccgtcggtca ccggtcttcg
     1561 gtggtgctcc cggtcgttcc caatccgacg ccgacacccg cggtgcggcc gccctgcgga
     1621 tcgacgcgcg ggcaaccgtg tcgcccgatc gcgtcgaagg cgatcgcgac gaacgtcacg
     1681 gcgcatcaga acgggggcga cggcgcaatc gtgacctgga ccgcacccgt cccgcgtgac
     1741 gacgacgcga ccgcgaccgg cattgccgcg caccaggtcg tggcccagcc gggcggcgcg
     1801 accgtgaccg tcgatcccaa ccagaccttg gccaccttcg ttgacatgcc ggacggcacc
     1861 tactcgttca cggtctacgc cggcggcgtg gccgcgagca caccgtcgca gtcgatcacg
     1921 atcactgacg tgccgacgac gacgagcaca acgagcacaa cgagcacaac gagcacaacg
     1981 acgtcgacca cgtcgacgtc aaccagcaca accagcacga cgacatcgac caccaccacg
     2041 gtgccggtga cgaccacgac ggtccccgac acgacgtcga ccacgttggc gccgtccgcg
     2101 accacgagca ccaccgtcgc ggtgggcacc gagggaacca ccgtgccgcc ggtcgtcgag
     2161 ggcaacgaca ttgacagcgg aggtggcgag tccggcggcg ggtcactccc gcgcaccggc
     2221 gcgtcgttgg cgctgctcgt gctgctcggc acggccttcg ttgtcgtcgg tttgatcgcc
     2281 cggcgccggt ccccggaggc cgcgcacacc gcgtcttcta gactccgcgg ccgatgaccg
     2341 acgttgccgc tgcccccgcg aaagaacggt ggagcttctc ctggaagcac ttgtatgacg
     2401 aggtcgtgac ctcggggttg tgtaccggat gcgcaggctg tgtgatcgcc tgcccgcacg
     2461 acgtgctcgg ctacgacgac acgaacggcg tgtacaagcc gcagcagatc gaggactacg
     2521 gcggcccgtc cgactgtagc cacggcgaca aggcgtgcac ctcgtgcaca cgcgcgtgcc
     2581 cgaggttccg cgcgtggga
//
LOCUS       DASUGA010000499         3101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_641312, whole genome shotgun sequence.
ACCESSION   DASUGA010000499 DASUGA010000000
VERSION     DASUGA010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641312"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VFX21_09810"
     CDS             complement(<1..239)
                     /locus_tag="VFX21_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096300.1"
                     /translation="MRKALIALFVVVLTGVAVAGVAWAANDNSKSAAHPRQDVLADTR
                     RATNQYRDVSAAIADGFADLGLCVPMMGEHYTLGG"
     gene            complement(284..568)
                     /locus_tag="VFX21_09815"
     CDS             complement(284..568)
                     /locus_tag="VFX21_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020514578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEX5096301.1"
                     /translation="MPRFVVERTFAGGLDIPTSSDGSTVCRAVVNNNAEEHVTWVTSY
                     VTNDRKKSFDVYDGPSPGAIQRAATANGLPVDTISEVQVLDPYFYLGPES"
     gene            685..2304
                     /locus_tag="VFX21_09820"
     CDS             685..2304
                     /locus_tag="VFX21_09820"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5096302.1"
                     /translation="MDVGVLGPVRAHTDSGEVVLSAAKERSVLATLALHAGAVVTPDA
                     LIDALWGATPPESARKTLQTYVSNIRRALGADVVGTDPNGYVLHVDRDAVDVLRFRRL
                     VREGEDALRAGATLHARDALTAATALWRGEPFGGVAAHTGLAAEAVRLREEYLGALEG
                     RVASELETGADAHLVGELEALVREHPYRERLWGHLMTALYRSGRQADALSVYERVRVL
                     LRDELGLEPGGELQRLQRAVLEHTMAERAVVPPSLQPFRSSVRYAVARDGVHVAYQVV
                     GDGPIDLIAVPGFVSHLDMWWDAPTDALVRRLASFSRLIMFDKRGMGLSDRPAHIDID
                     DWVEDTRAVFEAAGSRRAVVLGISAGAPTASLFAARYPEHTSALVVYGGYPRILRSDD
                     YEFGFEREDVDEFIQQMQDEWGTGFGLSVLAPSLETDPAALEFWARLQTTSASPGAAA
                     RFLRALAEVDISDELPQINAPTLLVHVQRDANTPVEGARICRDLIPGARLVELDSDIH
                     LIWLSDVIEEITDEIEQFIVSSVLAGTREKR"
     gene            2301..3077
                     /locus_tag="VFX21_09825"
     CDS             2301..3077
                     /locus_tag="VFX21_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5096303.1"
                     /translation="MSDVLFDCTDHVATITLNRPDRLNAISGPMLAEVGRRLIECDSD
                     RDVRVIVITGAGRGFCSGLDLQAVGGGGDGDLAAGANFRLDDTPPFALRRVDTPVLCA
                     LNGPAAGYGMDLALGCDVIVASDAASFTSPIRRGVVPESGGTWLLPRLVGWHKASEVL
                     LLGRKLDAPELERLGLVNTVVPADEFPAVVEKWASELAAQAPLATRTAKRLMRHGLDS
                     TFDTNGHEVMAELMGLFRTADFKESVQAFLEKRAPEYKGR"
BASE COUNT          481 a         1032 c         1079 g          509 t
ORIGIN      
        1 tccccgccca gcgtgtagtg ctcgcccatc atcgggacgc agagaccgag gtccgcaaac
       61 ccgtccgcga tcgcggcact gacgtcgcgg tactggttcg tcgcgcgccg ggtgtcggcg
      121 agcacatcct gtcgaggatg cgcggccgat ttcgagttgt cattggcggc ccacgcgacg
      181 ccggccacgg caacgcccgt gaggacgacg acgaagaggg cgatgagagc ttttcgcaat
      241 gttgtctcct tcaatgcacg cggtggtcgc cgcgcagcgg atgtcacgat tcgggaccga
      301 ggtagaagta cggatcgagc acctgtacct cggagatcgt gtcgacgggc aagccgttgg
      361 cggtcgccgc gcgctggatc gcgcccggtg agggaccgtc gtagacgtcg aacgacttct
      421 tccggtcatt cgtgacgtac gacgtcaccc acgtgacgtg ttcttcggcg ttgttgttga
      481 cgacggcgcg gcacacggtc gacccgtcgg acgacgtcgg gatgtcgagt cctccggcga
      541 acgtgcgctc gacgacgaat cgcggcattg gggatccttc ctcgaaccac cgttactggc
      601 ggccgcttcg aagagaaaca cggcgcggtc gggcgccggt cgagccgtgg tggagcgcgc
      661 cgatcgctcg taacatcgcc ggcaatggac gtcggcgtgt tggggccggt gcgagctcac
      721 accgacagcg gtgaggtcgt gctcagcgcc gccaaggaac ggtcggtgtt ggcgacgctc
      781 gcgttgcacg cgggcgcggt cgtgacgccc gacgcgttga tcgacgcctt gtggggagca
      841 acgccgccgg agagcgcgcg caagacgttg cagacatatg tgtcgaacat ccggcgcgcg
      901 ctcggcgccg acgtcgtcgg caccgacccg aacggctatg tgctgcacgt cgatcgcgat
      961 gcggtcgacg tgctgcgctt ccggcgtctc gtgcgagaag gtgaagacgc gcttcgtgcc
     1021 ggcgcgacgc tgcatgcacg cgacgcgctg accgccgcga ccgcgctctg gcgcggcgaa
     1081 cccttcggcg gagtcgccgc gcacacgggg ttggccgccg aagccgtgcg gctccgggag
     1141 gagtatctcg gcgcgctgga gggccgcgtc gcgtccgagc tggagaccgg ggcggacgca
     1201 caccttgtcg gcgagctcga agcgctcgtt cgcgagcacc cgtaccggga acggctgtgg
     1261 ggtcatctca tgaccgcgct gtaccgcagc gggcggcagg ctgacgcgct ctcggtgtac
     1321 gaacgagtgc gggtcttgct gcgcgacgag ctcggactcg aaccgggtgg tgagctgcaa
     1381 cgtctccagc gcgccgtgtt ggaacacacg atggcggaac gagcggtcgt gcccccgtca
     1441 ctgcagccgt tccggtcgtc ggtacgttat gcggtcgccc gcgacggcgt gcacgtcgca
     1501 taccaggtcg tcggcgacgg tccgatcgac ctcatcgcgg tgcccggatt cgtctcccac
     1561 ctcgacatgt ggtgggacgc tcccaccgac gcgctggtgc gccgcctcgc gtcgttcagc
     1621 cggctcatca tgttcgacaa gcgcgggatg ggcctttccg atcgacccgc gcacatcgac
     1681 atcgacgact gggtcgagga cacgcgcgcc gtgttcgaag cagccggttc acgacgcgcg
     1741 gtggtgctcg gtatctcagc tggtgcaccg accgcgtcgc tcttcgccgc gcgctaccca
     1801 gagcacacga gcgcactcgt cgtctatggc ggttacccgc gcatccttcg ctccgacgac
     1861 tacgagttcg ggttcgagcg cgaggacgtc gacgagttca tccagcagat gcaagacgag
     1921 tggggcaccg gattcgggct ctcggtgctc gcaccgagcc tcgagacgga cccggcagcg
     1981 ctcgagttct gggcgcgcct gcagacgaca tcggccagcc cgggcgcagc cgcgcggttc
     2041 ctgcgcgcgc tggctgaggt cgacatctcg gacgaacttc cacagatcaa cgcgcccacg
     2101 ttgctcgtgc acgtgcaacg cgacgcgaac acgcccgtcg agggcgctcg catctgtcgc
     2161 gatctcatcc cgggcgcacg actcgtcgag ctcgacagcg atatccacct catctggctc
     2221 tcggacgtga tcgaggagat caccgacgag atcgaacagt tcatagtctc cagtgtcctc
     2281 gccggtacaa gggagaagcg atgagcgacg tcttgttcga ctgcaccgac cacgttgcga
     2341 cgatcacgct caaccggccc gaccgcctca acgcgatcag tggacccatg ctcgcggagg
     2401 tcgggcggcg gttgatcgag tgcgactccg atcgcgacgt gcgcgtcatc gtgatcaccg
     2461 gcgcgggccg cgggttctgt tcgggactcg acctgcaggc ggtcggtggt ggcggcgacg
     2521 gcgatctcgc cgcgggcgcg aacttccgcc tcgacgacac accgccgttc gcgctgcgcc
     2581 gcgtcgacac gccggttctc tgcgcgctga acgggcctgc ggccgggtac ggcatggatc
     2641 tcgcgctcgg ctgcgacgtg atcgttgcgt ccgacgccgc gagcttcacc tcgccgatcc
     2701 ggcgcggtgt ggtgcccgag agcggtggca catggctgtt gccgcggctc gtgggctggc
     2761 acaaggcgag tgaagtgctc ttactggggc gcaagctcga cgcgccggag ctcgagcggc
     2821 tcggtctcgt caacaccgtc gtgcccgccg acgagttccc ggccgtcgtc gagaagtggg
     2881 cgtcggagct ggccgcgcaa gcgccgcttg cgacccgcac cgcgaaacga ctgatgcgcc
     2941 acggcctcga ctcgacgttc gacacgaacg gccacgaggt catggccgaa ctgatgggcc
     3001 tcttccgcac cgccgacttc aaggagagcg tgcaagcgtt cctcgagaag cgcgcccccg
     3061 agtacaaagg gcgctagtcg gcgcgcgcgg gtgccgcgac t
//
LOCUS       DASUGA010000500         3000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1891955, whole genome shotgun sequence.
ACCESSION   DASUGA010000500 DASUGA010000000
VERSION     DASUGA010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891955"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..319)
                     /locus_tag="VFX21_09830"
     CDS             complement(86..319)
                     /locus_tag="VFX21_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096304.1"
                     /translation="MPDEKKERAGLGFVGWTILLVLALVGGWVVVNNAIEWAKPWWRY
                     VTVASIAFTIGTFYGRYRRDQAAAKKKNKQQPF"
     gene            complement(312..1169)
                     /locus_tag="VFX21_09835"
     CDS             complement(312..1169)
                     /locus_tag="VFX21_09835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX5096305.1"
                     /translation="MKFGLMFANAGPFSKPENALGLARLCEDLGFESLWTVEHVVVPS
                     GYESAYPYSPDGKMPGGEHVPIPDPLTWLAFVAGATTKIRLGTGILILPQRNPLILAK
                     EVATLDRLSGGRVELGVGVGWLEEEFDALGVPWAARGRRTDEYIDVLRKLWREPEIEY
                     DGEFVKFAPLKSSPKPARDTGVPIHVGGHTMAAARRAGRSGDGFFPGRSGDELGDLLA
                     EMRRAATEAGRDADEIEITSGGGLDLDAVKSAADLGVSRYIIPPLGFDLDTLREQLGR
                     FSENVIARA"
     gene            1198..1851
                     /gene="npdG"
                     /locus_tag="VFX21_09840"
     CDS             1198..1851
                     /gene="npdG"
                     /locus_tag="VFX21_09840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEX5096306.1"
                     /translation="MRIGILGATGPAGGGLAARLASVGHEVLFGSRAVEKARGAVDEL
                     EKRWGDRVAGRLIACDNSWACDAPVVVLAVHADSAIPTVQEHAERLPGKVVVSMANNL
                     VKHGNEFNAVLPPHGSVAAEIQALLWRSRVCTAFHLVPAAEFADLDHTMHSDVVVLGD
                     EDEAKSTLMEITSSIPELRPLDGGSLRNAVGMETFAAVLLTVNIRHKMRASLRLTHV"
     gene            1848..>3000
                     /gene="cysS"
                     /locus_tag="VFX21_09845"
     CDS             1848..>3000
                     /gene="cysS"
                     /locus_tag="VFX21_09845"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX5096307.1"
                     /translation="MTMRLYDTARRAVVPFDPPRIVRMYVCGITPYDSTHLGHAATYL
                     TSDLLIRRLEDLGHEVRYVRNITDVDDSILPKARELGVPYLDLAESEMARFRGDMTAL
                     DMRPPIAEPRATESIDGIINLINELLESGHAYLTHGTAYFDVSSFPRFGELSHYSEEQ
                     MVRLARVRGGNPDDPHRRAPLDFVLWQPSAPDEPAWRAPFGVGRPGWHIECSAMAMHE
                     HGDTLDLHGGGTDLIFPHHECEIAQSESVTGKPFAKHWLHSAMVNYEGEKMSKSLGNL
                     VFVSDLLKSTDPRAIRLALMRHHYRHGFEWYDTDLAEGTALLHRLLAAAERPTGPDPR
                     PYAQRVRDAIDDDLDIQRALDALDDLASAILSGGDDENAHTVLCELGQLL"
BASE COUNT          482 a         1019 c         1028 g          471 t
ORIGIN      
        1 aggtacgcga gaacagacgg ccgcgccgca cccggcacgc ccgcgtggga cttcccggcg
       61 gccatcactc ccgtgatcaa gacgatcaaa acggttgctg cttgttcttc ttcttggcgg
      121 cggcctggtc gcggcggtag cggccgtaga acgtgccgat ggtgaacgcg atcgacgcga
      181 cggtgacata gcgccaccat ggcttggccc actcgatcgc gttgttgacg acgacccagc
      241 cgccgacgag cgcgaggacg agcaggatcg tccagccgac gaagccgagg ccggcgcgct
      301 ccttcttctc gtcaggcacg ggcgatcacg ttctcggaga agcgaccgag ctgctcgcgc
      361 agcgtgtcga ggtcgaagcc gagcggcggg atgatgtaac gcgacacgcc gaggtcggcg
      421 gcggacttga ccgcgtcgag gtcgaggccg ccgccggagg tgatctcgat ctcgtcggcg
      481 tcgcgtcctg cctcggtcgc ggcgcgccgc atctcggcca acaggtcgcc gagctcgtcg
      541 cccgaacgtc cggggaagaa cccgtcgccc gaccgtcccg cgcgccgcgc ggcggccatc
      601 gtgtgcccgc cgacgtggat cggcacgccc gtgtcgcgcg cgggcttcgg tgacgacttc
      661 agcggcgcga acttgacgaa ctcgccgtcg tactcgatct ccggctcacg ccacagcttg
      721 cgcaacacgt cgatgtactc gtcggtgcgg cgcccgcgtg cggcccacgg cacaccgagc
      781 gcgtcgaact cttcctcgag ccagccgacg ccgacgccga gctcgacgcg accacccgag
      841 agccggtcga gggtcgcgac ctccttggcc aagatcagcg ggttgcgctg cggcaggatc
      901 aagatgccgg tgccgaggcg gatcttcgtc gtcgcgccgg cgacgaacgc caaccacgtc
      961 aacgggtcgg ggatcgggac gtgctcgccg ccgggcatct tgccgtcggg agagtacggg
     1021 tacgccgatt cgtagccgct cggcacgacg acgtgctcca cggtccacag cgactcgaac
     1081 ccgaggtctt cacacaagcg ggcgaggccc agcgcgttct cgggcttgga gaagggcccg
     1141 gcgttggcga acatcagccc aaacttcacg ttcgcacgct accggtaatc tgcgccgatg
     1201 cggatcggca tcctgggtgc gaccggcccc gcgggcggtg ggctggcggc ccgtctcgcc
     1261 agcgtcggtc atgaagtgct gttcgggtct cgtgcggtcg agaaggcgcg cggcgccgtc
     1321 gacgagctcg agaaacggtg gggcgaccgg gtcgccggcc ggctcatcgc gtgcgacaac
     1381 tcgtgggcgt gcgacgcgcc ggttgtcgtg ctcgcggtgc acgcggactc ggcgatcccg
     1441 acggtccagg agcacgcgga gcgcctgccc ggcaaggtcg tcgtgtcgat ggcgaacaac
     1501 ctcgtgaagc acggcaacga gttcaacgcg gtgctgccgc cgcacggttc ggttgcggcc
     1561 gagatccagg cgctgttgtg gcgctcacgg gtgtgcacgg cgttccacct cgtgcccgcg
     1621 gcggagttcg ccgacctcga tcacacgatg cacagtgatg tcgtcgtgct cggcgacgag
     1681 gacgaggcga agtcgacgct gatggagatc acctccagta tccccgagtt gcggccgctc
     1741 gacggcggat cgctgcgcaa cgcggtcggc atggagacgt tcgcggcggt gctgttgacc
     1801 gtcaacatcc ggcacaagat gcgggcgagc ttgcggctca cacacgtatg acgatgcggc
     1861 tgtacgacac cgcgagacgg gcggtcgttc cgttcgatcc gccgaggatc gtgcgcatgt
     1921 atgtgtgcgg catcacgccg tacgactcga cgcacctcgg gcacgcggcg acgtacctca
     1981 cctccgacct gttgatccgc cgactcgaag acctcgggca cgaagtgcga tacgtgcgca
     2041 acatcaccga cgtcgacgac tcgatcctgc ccaaggcgcg cgagctcggc gtgccctatc
     2101 tcgacctcgc cgagtcggag atggcgcgct ttcgcggcga catgaccgca ctcgacatgc
     2161 ggccgccgat cgcggagccg cgcgcgaccg agtcgatcga cggcatcatc aatctcatca
     2221 acgagttgct cgagtcgggt cacgcgtacc tcacgcacgg caccgcgtac ttcgacgtct
     2281 cgtcgttccc gcgtttcggc gagctgtcgc actactctga ggagcagatg gtgcggctgg
     2341 cccgggtgcg gggcggcaac cccgacgatc cgcaccggcg agcgccgctc gacttcgtgc
     2401 tctggcagcc ctcggcgccc gacgagccgg cctggcgcgc accgttcggc gtcggccgcc
     2461 cggggtggca catcgagtgt tcggcgatgg cgatgcacga gcacggcgac acgctcgatc
     2521 tgcacggcgg tggcaccgac ctgatcttcc cgcaccacga atgcgagatc gcgcagagcg
     2581 agtcggtgac cggcaagccg ttcgcgaagc actggttgca ctcggcgatg gtcaactacg
     2641 agggcgagaa gatgtcgaag tcgctcggca atctcgtgtt cgtcagcgac ttgttgaagt
     2701 cgacggatcc gcgcgcgatc cggcttgcat tgatgcgaca ccactaccgg cacggcttcg
     2761 agtggtacga caccgatctc gccgaaggta cggcgttgtt gcaccgcttg ctggctgcgg
     2821 ccgaacgccc gaccggtccc gacccgcgtc cgtacgccca acgcgtgcgc gacgcaatcg
     2881 acgacgatct cgacatccaa cgcgcgctcg acgcgctcga cgacctcgcg agcgcgatcc
     2941 tgtcgggcgg tgacgacgag aacgcgcaca ccgtgttgtg cgagctcggt cagttgctgg
//
LOCUS       DASUGA010000501         3611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1571270, whole genome shotgun sequence.
ACCESSION   DASUGA010000501 DASUGA010000000
VERSION     DASUGA010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571270"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="VFX21_09850"
     CDS             <1..1001
                     /locus_tag="VFX21_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5096308.1"
                     /translation="CVLAYTSGTTSEPKGVMHTHRTLLAEIPHIEMWITPGAPNLMGS
                     PVTHATGMLGAVLAPMSLGEAIHLIDKWDPTRVLDVMIEADVGAGTGAAVFLASLLDH
                     PDFTPEHARRIKRVGLGGAPVAPALAERAAAQGIAMIRAYGSTEHPSVTGGRFDDPAA
                     KRHATDGRPMPGVEIELRDGEIWSRGPDLFVGYTDAAMTARAFDDDGWYRTGDMGVVD
                     DEGYLTITDRLNDVIIRGGENISAAEVEELAITLPQVAEVAVVAAPDERLGEHACAFV
                     RLVPDTSIDLATLTAHLADAGIARQKWPEELRVVADFPRTAAGKIRKIELREQLRA"
     gene            998..1837
                     /locus_tag="VFX21_09855"
     CDS             998..1837
                     /locus_tag="VFX21_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX5096309.1"
                     /translation="MTETYRTIKVAPLAGAIGAEISGVDLAHTDAETVAEIRAAWLTH
                     LVVFFRDQVLGPDEFLAFARQIGEPVEYPFVKGIEGYPDIISVTKLPHETVNFGGIWH
                     SDTVYLERPPMGTMLLAREIPPYGGDTLFANMYAAYEALTPGMQRLLDGLVAVNSSAL
                     ADVSKTREDRIGDSGAGADNEFVSEHPVVRTHPETGRKALYVNVAHTARFRDMSEEES
                     RPLLQYLFAHSVKPEFTCRFRWQVGSLALWDNRCAMHNPVNDYHGHLRSMHRITLAGD
                     VPR"
     gene            1834..3417
                     /locus_tag="VFX21_09860"
     CDS             1834..3417
                     /locus_tag="VFX21_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007451794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadD3 family acyl-CoA ligase"
                     /protein_id="HEX5096310.1"
                     /translation="MTRTEVTWPTIPAMLRDTADRLGTGDAIVSGDRRVTYAELRALV
                     TDAARSLIAAGVAPGDRVAIWAPNSIEWIVAALGITTAGAVLVPVNTRFKGSEAAYIL
                     ARSNARVLFTVRGFLDTDYPALLAAAGVALPALEHTVLLGGDASGATAWDDWLARGAD
                     VPATEVDARIAKLTSDDPSDVVYTSGTTGDPKGVVMTHGQTLRAYLDWCDWADLRAGD
                     RYLIVNPFFHIFGYKAGVLASLMRGATIFPLAVFDTDVVCEIVERERITVFPGAPTLY
                     QSLLDATDARAQHDISSLRLAVTGAADIPVELIRRVKEELPFERILTGYGLTEAGTVT
                     GSADGDDFEHIATTVGVPWPGFEVRTVTDTGADAGVDEPGEVIVRGATVMRTYLDDPE
                     ATAAAIDGDGWLHTGDLGTFDADGYLRIVGRIKDMFIVGGFNAYPAEIENLLLRHPRV
                     AQVAVIGVPDARLGEVAKAFVVLSPGDAVGSDEIIEWARAEMANYKVPRSVEFLDALP
                     VNATGKVVKDELRAREGST"
     gene            3417..>3611
                     /locus_tag="VFX21_09865"
     CDS             3417..>3611
                     /locus_tag="VFX21_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006538467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX5096311.1"
                     /translation="MAQVPVAEGIFTGPSDDPRLIGSRCAACGIVTFPAQDSCPRCAA
                     ADMVEEELPRRGRLWAWTTQH"
BASE COUNT          577 a         1269 c         1234 g          531 t
ORIGIN      
        1 tatgcgtgct cgcgtacacc tcggggacga cgagcgagcc gaagggcgtg atgcacacgc
       61 atcgcacgct cctcgccgag atcccgcaca tcgagatgtg gatcacaccc ggcgcgccca
      121 acctcatggg ctcgccggtg acgcacgcga cggggatgct cggtgccgtg ctcgcgccga
      181 tgagcctggg tgaggccatc cacctgatcg acaagtggga tccgacccgc gtgctcgacg
      241 tgatgatcga agccgatgtc ggcgcgggca cgggcgccgc agtgttcctg gccagtctgc
      301 tcgatcaccc cgactttacg cccgagcacg cgcgccgcat caagcgggtc ggcctcggcg
      361 gcgcgccggt cgcacccgcg ctcgccgaac gcgcggcggc gcagggcatc gcgatgatcc
      421 gcgcgtacgg atcgaccgag catccgtcgg tgaccggcgg ccggttcgac gatcccgcgg
      481 ccaagcggca cgcgaccgac ggacgcccca tgcccggtgt cgagatcgaa ctgcgcgacg
      541 gcgagatctg gtcgcgcggt cccgacctgt tcgtcggcta caccgacgcc gcgatgacag
      601 cacgcgcgtt cgacgacgac ggctggtatc gcaccggcga catgggcgtg gtcgacgacg
      661 agggttacct cacgatcacc gaccgcttga acgatgtcat catccgcggc ggcgagaaca
      721 tctcggctgc ggaagtcgag gagctcgcga tcacactgcc gcaagtcgcc gaagtcgcgg
      781 tcgtcgcggc acccgacgaa cgactcggcg agcacgcgtg cgcgttcgtg cgtctcgtcc
      841 ccgatacgtc aatcgacctc gcgaccctca ccgcgcatct ggcggatgcg ggcatcgcgc
      901 gccagaagtg gccggaagag ctgcgcgtcg tcgccgactt tccccgtacc gcagcgggaa
      961 agatccgcaa gatcgagttg cgggagcagc tgcgggcatg accgagacgt accgcacgat
     1021 caaggtcgcg ccgctcgccg gtgcgatcgg cgccgagatc agcggcgtcg acctcgccca
     1081 caccgacgcc gagaccgtgg ccgagatccg cgccgcgtgg ctcacccatc tcgtcgtctt
     1141 cttccgcgac caggtgctcg ggcccgacga gttcctcgcg ttcgcgcgcc agatcggcga
     1201 gcccgtcgag taccccttcg tgaagggcat cgagggctac cccgacatca tctcggtcac
     1261 gaaactgccg cacgagacgg tgaacttcgg cggcatctgg cacagcgaca ccgtgtacct
     1321 cgaacggccg ccgatgggca cgatgctgct ggcgcgcgag atcccgccgt acggcggcga
     1381 cacgctgttc gccaacatgt acgccgcgta cgaagcactc acgcccggca tgcagcggct
     1441 gctcgacgga ctcgtcgccg tcaacagctc ggctctcgcc gacgtctcca agacgcgcga
     1501 agaccgcatc ggcgacagcg gcgcgggtgc cgacaacgag ttcgtcagcg agcatccggt
     1561 cgtgcgcacg catcccgaga cgggccggaa ggcgttgtac gtcaacgtcg cgcacacggc
     1621 gcgcttccgc gacatgagcg aggaggagag ccggccgctg ctgcaatact tgttcgccca
     1681 ttcggtgaag cccgagttca cgtgccggtt ccgttggcag gtcggctcgc tcgcgctgtg
     1741 ggacaaccgc tgcgcgatgc acaacccggt gaacgactac cacggccacc tgcgttcgat
     1801 gcaccgcatc actctcgccg gggacgtgcc gcgatgacgc gcaccgaggt cacgtggccg
     1861 acgattcccg cgatgctgcg cgacaccgcc gaccggctgg gcaccggtga cgcgatcgtg
     1921 tccggcgacc gacgggtcac gtacgccgag ctccgcgcgc tcgtcactga cgcggcgcgc
     1981 tcgctcatcg cggccggtgt cgctccggga gatcgcgttg cgatctgggc gccgaactcg
     2041 atcgagtgga tcgtcgccgc gctcggcatc acgacggcgg gcgcggtgct cgtgccggtg
     2101 aatacgcgct tcaagggcag cgaggctgcg tacatcctcg cccgcagcaa cgcgcgcgtg
     2161 ctgttcaccg tgcgcgggtt cctcgacacc gactaccccg ccctgctcgc ggccgcgggt
     2221 gtcgcactac cggcactgga gcacaccgtc ctcctcggcg gcgacgcgag cggcgcgacc
     2281 gcgtgggacg actggctcgc gcgcggcgcc gatgtacccg cgaccgaggt cgacgcgcgc
     2341 atcgcgaagc tcaccagcga cgacccgagc gatgtcgtct acacctcggg cacgaccggc
     2401 gatcccaagg gtgtcgtgat gacacacggc cagacgctgc gggcgtatct cgactggtgc
     2461 gactgggccg atctgcgcgc cggtgaccgc tacctcatcg tcaatccgtt cttccacatc
     2521 ttcgggtaca aggccggcgt gctcgcgtcg ctgatgcggg gtgcgacgat cttcccgctc
     2581 gcggtgttcg acaccgacgt cgtgtgcgag atcgtcgaac gtgagcgcat cacggtgttt
     2641 ccgggcgcgc cgacgctgta ccagtcgctg ctcgacgcga ccgacgcacg cgcgcagcac
     2701 gacatctcga gcttgcggct cgcagtgacg ggcgcggccg acatccccgt cgagctgatc
     2761 cggcgcgtga aggaagagct gccgttcgag cgcatcctca ccggctacgg cctcaccgag
     2821 gccggcaccg tgaccggcag cgcggacggc gacgacttcg agcacatcgc gacgacggtc
     2881 ggcgtgccgt ggcccggctt cgaggtccgc accgtgaccg atacgggcgc ggatgccggc
     2941 gtcgacgaac cgggcgaagt gatcgtgcgc ggcgcgacgg tgatgcgcac gtacctcgac
     3001 gaccccgagg cgaccgcggc cgcgatcgac ggcgacggct ggctgcacac cggcgacctc
     3061 ggcaccttcg acgccgacgg ctacctgcgc atcgtcggtc gaatcaagga catgttcatc
     3121 gtcggcggct tcaacgcgta tccggccgag atcgagaacc tgttgttgcg ccacccgcgc
     3181 gtcgcgcagg tcgctgtgat cggtgtgccc gatgcccgtc tcggcgaggt ggcgaaggcg
     3241 ttcgtcgtct tgtcgcccgg cgatgccgtc ggcagcgacg agatcatcga gtgggcgcgc
     3301 gccgagatgg ccaactacaa agtgccgcgg tcggtcgagt tcctcgacgc gctgccggtc
     3361 aacgcgacgg gcaaagtcgt caaggacgaa ctgcgggccc gcgaagggag cacgtgatgg
     3421 cgcaagtgcc ggtcgccgaa ggcatcttca cggggccgag cgacgatccg cgactgatcg
     3481 gcagccgctg cgcggcgtgc ggcatcgtca cgttccctgc tcaggactcc tgcccgcgct
     3541 gcgcggccgc cgacatggtc gaggaggagc tgccgcggcg cggccggttg tgggcgtgga
     3601 cgacacagca c
//
LOCUS       DASUGA010000502         2679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1144624, whole genome shotgun sequence.
ACCESSION   DASUGA010000502 DASUGA010000000
VERSION     DASUGA010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144624"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1542)
                     /locus_tag="VFX21_09870"
     CDS             complement(<1..1542)
                     /locus_tag="VFX21_09870"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX5096312.1"
                     /translation="MSLRVGLALVLVLLFLAPPPPAGAAETAWVKPVDGRVVRPFDPP
                     KSRFGAGHLGVDFAAAPGTPVRSAGPGTVTFAGTVAGALHVVVAHAGGLRTSYSFLAT
                     TSVRRGQEVRAGAVVGTSGGTGTNHDGAVVHLGLRAGETYVDPMQLFTAVDLSEVVHL
                     APTSEPFGFTVAQERRGLLDGLGGALGGLWDTGAGALHTLGHASAVVLSEAWQPLAEY
                     AKWGGELAVRRIEHVLLNLPPLRATSVALTLLGAIKEYVWDGRNCDDHAPPADGTGGS
                     GHDAMVVAGIRTETGADGRTVDLPVDALGYQADEVTYFSYATGGGAYREADTYAPIMD
                     SARNLAAQLREHQVREPGREVDLLAHSQGGVVVRAFLAYFYDPGDPSFPPLGTVVTFA
                     SPLDGAPLATAVSQVQDVLFVGEPALDAIDALPIGPPDLDSAALRDLDEDSAFMRDLR
                     ASHLNGSIDITTIGATFDVVVPGQVATGAGARHIVVNTGVAGAHSGVLQDGDALRAAR
                     SALEGR"
     gene            complement(1642..2421)
                     /gene="whiG"
                     /locus_tag="VFX21_09875"
     CDS             complement(1642..2421)
                     /gene="whiG"
                     /locus_tag="VFX21_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HEX5096313.1"
                     /translation="MAATIAELWDEYKDGNGRDARERLILHYSPLVKFVAGRVAAGLP
                     QNIEQSDLVSYGIFGLIDAIDKFDPGRGYKFETYAISRIKGAIIDELRSIDWVPRSVR
                     AKARAIERAYSKLENELRRSPDDKEVATELGMSEEELATVLGQISFVGVVALDELLSA
                     GAERGPGATVGDTIAGSGHDPVEAFESEEMRQVLADAINRMPDRERLVLTLYYYEGLT
                     LAEIGSVLGVTESRVCQIHTKAIFQLRSRLSEPMPERGIVT"
     gene            complement(2447..>2679)
                     /locus_tag="VFX21_09880"
     CDS             complement(2447..>2679)
                     /locus_tag="VFX21_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX5096314.1"
                     /translation="PRDARRITERRLLTDGRAISPHSLRHAYATHLLEGGADLRAVQE
                     LLGHADVGTTQVYTHVTRDRLRTVYERTHPRA"
BASE COUNT          401 a          913 c          962 g          403 t
ORIGIN      
        1 ccggccctcg agggccgagc gggcggcccg gagcgcatcg ccgtcctgga gcacaccgct
       61 gtgggcaccg gcgacaccgg tgttcacgac gatgtggcgc gcacccgcgc cggtcgccac
      121 ctgcccgggt actaccacgt cgaaggtggc accgatcgtg gtgatgtcga tggagccgtt
      181 caggtggctc gcgcgcaggt cgcgcatgaa cgcactgtct tcgtcgaggt cgcgtagcgc
      241 agcactgtcg aggtcgggcg ggccgattgg cagcgcatcg atcgcgtcga gtgcgggttc
      301 accgacgaac agcacgtctt ggacctggct caccgcggtc gccaacggcg cgccgtcgag
      361 cggtgacgcg aacgtgacga cggtgccgag cggtgggaac gacggatcgc cggggtcgta
      421 gaagtacgcg aggaacgcac gcacgacgac gccaccttgg gagtgcgcga gcagatcgac
      481 ctcgcggccc ggctcgcgca cctggtgctc tcgcagctgt gcggccagat tgcgggcgga
      541 gtccatgatc ggcgcgtacg tgtcggcctc gcggtacgcg ccgccgccgg tggcatacga
      601 gaagtaggtg acctcgtcgg cctgatagcc gagcgcatcg acaggcaggt cgacggtacg
      661 gccgtcggcg ccggtctcgg tgcggatgcc ggcgacgacc attgcgtcgt ggccggagcc
      721 gccggtgccg tccgcgggcg gggcatggtc gtcgcagttg cgcccgtccc acacgtactc
      781 cttgatcgcg ccgagcaatg tcagcgcgac gctggtggcc cgcagcggcg gcaggttcaa
      841 cagcacgtgc tcgatgcgcc gcaccgcgag ctctccgccc cacttcgcat attccgcgag
      901 cggttgccag gcctccgaca agacgacggc gctcgcgtgg ccgagggtgt ggagcgcgcc
      961 ggcgccggtg tcccacaacc cgccgagcgc gccaccgagg ccgtcgagca gacctcgccg
     1021 ttcctgcgcg accgtgaacc cgaacggttc gctggtcggc gcgaggtgca cgacctcgct
     1081 caagtcgacg gcagtgaaca actgcatcgg gtcgacgtag gtctcgccgg cgcgcaaccc
     1141 gaggtgcacg accgcaccgt cgtggttcgt gcccgtgccc ccgctcgtgc cgacgaccgc
     1201 gcccgcgcgc acctcctgcc cgcgccgcac cgaggtggtg gcgaggaacg aatacgacgt
     1261 gcgcaggccg cccgcgtggg cgacgacgac gtgcagcgcg cccgcgaccg tgcccgcgaa
     1321 cgtgacggtg cccggaccgg cgctgcgcac cggcgtgccg ggcgcggcgg cgaagtcgac
     1381 gccgagatgg ccggcgccga agcgcgactt cggcgggtcg aaggggcgga cgacccggcc
     1441 gtcgaccggc ttcacccacg cggtctcggc cgcgcccgcg ggcggcggcg gagccaggaa
     1501 caacaggacg agaacgagcg cgagaccgac tcgcaggctc atgaggcctc cccgagcgca
     1561 acgtgacaac ggcgcgggga aggcgagagg aagtgctgcg cacgctagtg cgcgggtgcg
     1621 acagttccta gggcgactgc gtcaggtgac gatgccgcgt tcgggcatcg gttcgctcaa
     1681 cctcgatcga agttgaaaga tcgccttcgt gtggatctga cacacgcggc tctcggtcac
     1741 accgaggacg ctgccgatct cggcgagcgt gagcccttcg tagtagtaga gcgtgagcac
     1801 cagtcgttca cggtcgggca tccggttgat cgcgtcggcg agcacctgcc gcatctcttc
     1861 ggactcgaac gcttcgacgg ggtcgtgccc cgagcccgcg atcgtgtcgc cgaccgtcgc
     1921 acccgggccc cgttcggcgc ccgcactgag cagctcgtcg agcgcgacga caccgacgaa
     1981 gctgatctgg ccgagcaccg tcgccagctc ttcctccgac atgccgagct cggtcgcgac
     2041 ctctttgtcg tcggggctgc gccgcaactc gttctcgagc ttggagtacg cccgttcgat
     2101 ggcccgggcc ttggcgcgca ccgagcgcgg cacccagtcg atcgagcgaa gctcgtcgat
     2161 gatggcgccc ttgatgcgcg agatcgcgta cgtctcgaac ttgtagccgc ggccgggatc
     2221 gaacttgtcg atcgcgtcga tcagcccgaa gatgccgtag ctgacgaggt cggactgttc
     2281 gatgttctgg ggcagcccgg cggccacccg accggccacg aacttgacca gcggcgagta
     2341 gtgcaggatc agccgttcgc gcgcgtcgcg accgttgccg tccttgtact cgtcccagag
     2401 ctcggcgatc gtggccgcca gctcgtccac cgcagcgggg ctcttgtcag gcacgcggat
     2461 gtgtccgttc gtacacggtt ctcaatcggt cccgagtcac gtgagtgtaa acctgcgtgg
     2521 tccctacatc ggcgtgaccg agcagctcct ggacggcccg gaggtccgct cccccctcca
     2581 gaaggtgggt ggcgtaggcg tggcggaggc tgtgcgggct gatggctcga ccgtccgtga
     2641 gcaggcgacg ttcggtgatt cggcgggcgt cgcggggtg
//
LOCUS       DASUGA010000503         5033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_401487, whole genome shotgun sequence.
ACCESSION   DASUGA010000503 DASUGA010000000
VERSION     DASUGA010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401487"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VFX21_09885"
     CDS             <1..647
                     /locus_tag="VFX21_09885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5096315.1"
                     /translation="PPRAGRIVLEVEDLAKAFGPKVVFEDITFDLERGERLLVLGLNG
                     AGKTTLLKSIIGELEPELVEVRLGHNASVGYYAQEHEGITPGRSVLEHVREQSGLADQ
                     YLRSLLGMFGLRGDLAFQDAGTLSGGEKTKLALTMLVTGGHNVLILDEPTNNLDPPSR
                     TAVGAALRNWKGAMLLVSHDTEFVTELMPDRVLLMPEGTVDYWSDDLVDLVAMA"
     gene            1102..2637
                     /locus_tag="VFX21_09890"
     CDS             1102..2637
                     /locus_tag="VFX21_09890"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5096316.1"
                     /translation="MLLAGALAASACSSSGGDDDDSAESSGGGDRILRYGYDMSAQFT
                     NTFDIATSKGDCDSIVLAPIYDTLVHKKPGNVLEPGLLEKWEVIDASTLDLYLREGLV
                     FSDGTPLNADALQQGLEHNADNDQLSDLRKIDSFEKIDDTTLRVKMKKPIAVQMPATF
                     SSRDGMIMKPGSTSDAPIGAGPFVLTKSDPGSVISVRTNDKYWNKDVYEGLDGVDYTQ
                     TGTGPPAVTALLAGDLDLVRMEAESYADLKDKDGYEVVVQPTGAYLQFEFRQSYKDAP
                     TPFANVQVRQAVSHAIDREKINEVVQDGLGEVATQPFPEDSDAYVPEVADLYPYDPDQ
                     AKQLLTEAGYPDGFEFTMAIPGGNITNMERMSTLVQEQLGAVGIKANIKRILGTDIAT
                     QYYILGGGDAFSAAELDTTFPTGKLQNNYGVGEFVAIWDGAEDQAMTDLMTEAQGTTD
                     ENESYALVRQGVTTAMQNALDVPIAFMPQLMAYNSDRVSGTVGGQTNICDPPDLTAAV
                     VKG"
     gene            2662..3609
                     /locus_tag="VFX21_09895"
     CDS             2662..3609
                     /locus_tag="VFX21_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5096317.1"
                     /translation="MIGYVARRLLALIPLLFIISFVVFGMTLLVPGDPAIEIAGGINA
                     EPADVARVREELHFNEPVLVQYGYWLRDAVHGDLGQSYLRNSSVTDELQRRIPVTLSM
                     ALGALFVTVMLGLPAGIIAGTRPRSPRDRGVTVASSVGVALPDFWLATIFVVWFAVDL
                     GWFPAQGYVPPAESVTEWAKHLVLVWFALGIAGAASFARQLRGALIDTLEQDYIRTAQ
                     AKGAGKRRVVYKHALKNASFIPITVLGLQFAYLLGGTVILEYIFSLNGIGGYFYTALV
                     SRDIPVIQGVTLFVAVTFVVVNLVIDLVYGYLNPKVRLA"
     gene            3606..4550
                     /locus_tag="VFX21_09900"
     CDS             3606..4550
                     /locus_tag="VFX21_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5096318.1"
                     /translation="MSILPGAPVFSEHETENARIAEAEEVTAKSESPRRRLWRRFRHN
                     WLAMGALVYLVVLVVVSVFAGFFAPHSPYATGGPINSGPSREYWLGTDSTGRDILSRL
                     IYGGRSSLEVCFLVVLFALLIAVPLGLISGYFRGWPDSVISRVMDGLFAFPALTLALA
                     IAAILGPSLRNAAIALSIPFIPGMVRLLRAQVLGVREETYIEASVSVGATRRRMLTHH
                     VGPNVASPMIVQLALAFGFALLAEAGLSFIGLGVQPPQPSWGAMIGDAQKFQLSAPWP
                     IYPPGIAIALTVLAFNLIADGLRDSMGRESFRLSAVAS"
     gene            4655..>5033
                     /locus_tag="VFX21_09905"
     CDS             4655..>5033
                     /locus_tag="VFX21_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5096319.1"
                     /translation="MLRVVEDVSFGVGTGEVLGLVGESGSGKTVTSLSILGLHPRDGT
                     SVSGAIRFGGRDLLRTGDAELRAIRGNEIAMIFQEPMTSLNPAFTVGNQIAEAVRLHT
                     DASRKEAWRRAVEMLDRVGIPGAG"
BASE COUNT          799 a         1671 c         1706 g          857 t
ORIGIN      
        1 atccgccgcg cgccggtcgg atcgtgctcg aagtcgagga tctcgccaag gcgttcggcc
       61 cgaaggtcgt cttcgaagac atcaccttcg acctcgaacg cggcgaacgc ctcctcgtgc
      121 tcggcctgaa cggcgcgggc aagacgacgc tgctcaagtc gatcatcggt gagctcgagc
      181 ccgagctcgt tgaagtgcgc ctcgggcaca acgcgtcggt cggctactac gcgcaggaac
      241 acgagggcat cacgcccggt cgttcggtgc tggaacacgt gcgggagcag tcgggtctcg
      301 ccgaccagta cttgcgcagc ctcctcggca tgttcgggtt gcggggcgac ctcgcgttcc
      361 aggacgcggg cacgctctcc ggcggtgaga agacgaagct cgcgttgacc atgctcgtga
      421 ccggtggcca caacgtcttg atcctcgacg agcccaccaa caacctcgat ccgccctcgc
      481 gcaccgcggt cggtgctgcg ttgcgcaact ggaagggcgc gatgctgctc gtcagtcacg
      541 acaccgagtt cgtgaccgag ctcatgcccg accgcgtgtt gctgatgccc gaaggcaccg
      601 tcgactactg gtcggatgac ttggtagacc tcgtcgcgat ggcgtaattc ctggtcgctc
      661 cggcggcgcg cgcgtgtgcg gcttcggtgc gttgttgccg cctccggcgc cgcgagtggc
      721 cgagacggtc gtggggggcg ttgcgcgccg tgtggggacg tgaggccacc gccgctctga
      781 cgtctcgcgc cggtcctcga ggggcgctgc gcgccgcggg acgaccgaac ttcgcgatca
      841 ttgcttctcg ctcgggtcgt gctcgcgagg ggtagccgac ttcgggctgc ccgaattgtg
      901 aacgcgtgct tgcaatccgc agcatgccgg cgaccgtgtt gcgatacggc atcgtcttcg
      961 ttttccgcat tgccgagaca aactgacgcg cgtgtcatag agtccgcgcc ggatggcgtg
     1021 agggggttcg atatgcgcgt cccgtgtgct gccggccgtg tcgaaagacg tgggatccgt
     1081 aggcgtcgct cgtacgtcgc cgtgttgctc gccggcgcgc tcgccgcgtc cgcgtgcagc
     1141 tcgagcggtg gtgacgacga cgacagcgcc gagagcagtg gcggaggcga tcgcatcctg
     1201 cgctacggct atgacatgtc ggcgcagttc acgaacacgt tcgacatcgc aacgagcaag
     1261 ggagactgcg actcgatcgt gctggcgccg atctacgaca cgctcgtgca caagaagccg
     1321 ggcaacgtgc tcgaacccgg cctgctcgag aagtgggaag tcatcgacgc gagcacgctc
     1381 gacctctact tgcgcgaggg cctggtgttc tccgacggca cgccgctgaa cgcagacgcg
     1441 ctgcaacaag gcctcgagca caacgccgac aacgaccagc tgtccgatct gcgcaagatc
     1501 gactcgttcg agaagatcga cgacacgacg ctgcgagtga agatgaagaa gccgattgcc
     1561 gtgcagatgc ccgcgacgtt ctcgtcgcgc gacggcatga tcatgaagcc gggctcgacg
     1621 agcgacgcac cgatcggcgc gggaccgttc gtgctcacca agagcgaccc gggcagcgtc
     1681 atctcggtgc gcaccaacga caagtactgg aacaaggacg tctacgaagg gctcgacggc
     1741 gtcgactaca cgcagaccgg taccggtccg cccgcggtca ctgcgctgct cgccggtgac
     1801 ctcgacctcg tccgcatgga ggcggagtcc tacgccgact tgaaggacaa ggacggttac
     1861 gaggtcgtcg tgcaacccac cggcgcctac ctgcagttcg agttccgcca gagctacaag
     1921 gatgcgccga ccccgttcgc gaacgtgcag gtgcgccaag cggtatcgca cgcgatcgac
     1981 cgcgagaaga tcaacgaggt cgtccaggac ggtctcggcg aggtcgcaac gcaaccgttc
     2041 ccggaggatt ccgacgcgta cgtccccgag gtcgccgacc tttacccgta cgaccccgac
     2101 caggcgaagc agctgctgac cgaagcgggc taccccgacg gtttcgagtt cacgatggcg
     2161 atccccggcg ggaacatcac gaacatggaa cggatgtcga cgctcgtgca ggagcaactc
     2221 ggcgcggtcg gcatcaaggc gaacatcaag cgcatcctcg gcaccgacat cgcgacgcag
     2281 tactacatcc tcggtggcgg agacgcgttc tcggcggccg agctcgacac gacgttcccg
     2341 accggcaagc tgcagaacaa ctacggcgtc ggtgagttcg ttgcgatctg ggacggcgcc
     2401 gaagaccagg cgatgaccga cctgatgacg gaggcgcagg gaaccaccga cgagaacgag
     2461 tcgtacgcgc tcgtacgcca aggcgtgacc acggccatgc agaacgcgct cgacgtgccg
     2521 atcgcgttca tgccgcagct catggcgtac aactccgacc gggtgagcgg cacggtcggc
     2581 ggccagacga acatctgcga cccgcccgac ctgaccgccg cggtggtgaa gggctagcgg
     2641 gaccgcagtg ggcgacgcgt cgtgatcggg tacgtcgccc gccgcctgct ggcgctgatc
     2701 ccactgctgt tcatcatctc gttcgtcgtg ttcgggatga cgttgctggt gcccggtgat
     2761 cccgccatcg agatcgcggg gggcatcaac gcggagcccg cggacgtcgc acgggtccgt
     2821 gaggagctgc acttcaacga gccggtcctc gtgcagtacg ggtactggct gcgcgacgcc
     2881 gtgcacggag acctcggtca gtcgtacttg cgcaactcga gtgtcaccga cgagttgcag
     2941 cgccggatcc cggtcacgct cagcatggcg ctcggcgcgc tcttcgtcac ggtgatgctc
     3001 gggctcccgg cgggaatcat cgcgggcacg agaccgcgca gcccgagaga tcgtggggtc
     3061 accgtcgcga gcagcgtcgg cgtcgcgctc ccggacttct ggctcgcgac gatcttcgtc
     3121 gtgtggttcg ccgtcgatct cggctggttc ccggcccagg ggtacgtgcc gccggcggag
     3181 tcggtcaccg agtgggccaa gcatctcgtg ctcgtgtggt tcgcgctcgg catcgcgggc
     3241 gcggcgtcgt tcgcgcgcca actccgcggc gcgctgatcg acaccctcga gcaggactac
     3301 atccggaccg cgcaggcgaa gggcgcgggc aagcgacggg tggtctacaa gcacgcgctc
     3361 aagaacgctt cgttcatccc gatcaccgtg ctcggcctgc agttcgcgta tctgctcggt
     3421 ggcaccgtga tcctcgagta catcttctcg ctcaacggga tcggcggtta cttctacacc
     3481 gcgctcgtca gccgcgacat tcccgtcatc caaggtgtca cgttgttcgt cgcggtgacg
     3541 ttcgtggtcg tgaacctcgt gatcgacctc gtctacgggt atctcaatcc gaaggtgagg
     3601 ctcgcgtgag catcctgccg ggcgcgccgg tgttctcgga gcacgagacc gagaacgcgc
     3661 gcatcgcaga ggccgaagag gtcacggcga agagcgagtc gccgcgtcgg cgtctctggc
     3721 gccggttccg gcacaactgg ctcgcgatgg gcgcgctcgt ctatctcgtc gtgctcgtcg
     3781 tcgtgtcggt gttcgccggc ttcttcgcgc cccacagtcc gtatgccacg ggcggcccga
     3841 tcaacagtgg tccgagccgc gagtactggc tcgggaccga ctccaccggc cgcgacatcc
     3901 tcagccggtt gatctacggc ggccgttcgt cactcgaggt ctgcttcctt gtcgtcctgt
     3961 tcgcgttgct catcgcggtt cccctcggtc tcatctccgg ctacttccgg ggctggccgg
     4021 actccgtcat cagccgcgtc atggacggct tgttcgcgtt ccccgcgctc acgctcgcgc
     4081 tcgcgatcgc ggcgatcctg ggcccgagcc tccgcaacgc cgcgatcgcg ctgtcgatcc
     4141 cgttcatccc cggcatggtg aggctcctgc gcgcgcaggt gctcggcgta cgcgaggaga
     4201 cctacatcga ggcgtcggtc tcggtcgggg cgacccggcg gcgcatgctc acgcatcatg
     4261 tgggcccgaa cgtcgcgtca ccaatgatcg tccagcttgc gctcgcgttc gggttcgcgt
     4321 tgctggcgga ggcgggattg agcttcatcg ggctgggcgt gcaaccaccg cagccgagtt
     4381 ggggcgcgat gatcggcgac gcccagaagt tccagctgtc ggcgccgtgg ccgatctacc
     4441 cgccgggcat cgcgatcgcg ctgacggtcc tcgcgttcaa cctgatcgcg gacgggttgc
     4501 gcgacagcat gggccgcgag tcgttccggc tgagcgcggt cgcgtcgtga cgtcgctcga
     4561 cgcgcgcacc cccgcgcccg ccgacgccga tgccggcacc gggccactgc tgtcggtcga
     4621 gcatctcgac gtcgcgttcg cgaccggcgc cgggatgttg cgcgtcgtcg aggacgtgag
     4681 cttcggcgtc ggcaccggcg aggtgctggg actcgtgggg gagagcgggt caggcaagac
     4741 ggtgacgtcg ctgtcgatcc tggggttgca cccgcgtgac ggcaccagcg tcagcggcgc
     4801 gattcgtttc ggcggccgcg acctcttgcg caccggtgac gccgagcttc gcgcgatccg
     4861 cggcaacgag atcgcgatga tcttccaaga gccgatgacg agcctgaacc ccgcgttcac
     4921 ggtcggcaac cagatcgcgg aagcggtgcg cttgcacacc gacgcgagcc gcaaggaggc
     4981 gtggaggcgc gcggtcgaga tgctcgaccg cgtcggcatc ccgggcgccg gct
//
LOCUS       DASUGA010000504         3479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1327737, whole genome shotgun sequence.
ACCESSION   DASUGA010000504 DASUGA010000000
VERSION     DASUGA010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327737"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VFX21_09910"
     CDS             <1..434
                     /locus_tag="VFX21_09910"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEX5096320.1"
                     /translation="PTRTTPTRTLAEMAELAAGFALRVDGTELAGAEVVDGRRYVCLV
                     RTASYEAWLVEWSPASMLAPHDHGGAAGVLHVVDGELVETYRDDKKTKSRERKIEAGY
                     TVRVPASREHEVWNATARPARSIHVYSPPGLVSSALTAPAS"
     gene            445..1239
                     /locus_tag="VFX21_09915"
     CDS             445..1239
                     /locus_tag="VFX21_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5096321.1"
                     /translation="MPTIAANGIELYFERPGAGPRLLFLNGSGSTIETSRLLIQPFTE
                     RFEVLAFDQRGLGLSAIPPGPYEMADYAADAIGLLDAVGWPSCRVFGVSFGGMVAQEL
                     AVTAPDRIERLVLSCTSPGGAAGASYPLHELAAMPADEAAALGMRLLDTRFTPEYLAA
                     NAGDRGLAEMMVARRSAEKSDEQRRGEAEQMMARSRHDVVARLGAISAPTLVAAGRYD
                     GIAPLPNSEAIASRVPNAELRVYEGGHAFFAQDAKALPEIFDFLAQ"
     gene            complement(1243..1932)
                     /locus_tag="VFX21_09920"
     CDS             complement(1243..1932)
                     /locus_tag="VFX21_09920"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX5096322.1"
                     /translation="MSSRNRERRRLNAENREAEREYRVRHARNRRWAIAIGGLVIAAA
                     VAFFFVAKPFDDDDDSVSATDTPTTVAGSVAGKPCVAVVDPLPEGAPAVDVVVGPPPT
                     SLVIKDLTEGTGEVVPAGASITADYIGVACTTGKIFDSSYSRGEPTPFSLDGVIKGWT
                     DGIPGMKVGGRRLLGIPGDQAYGPAGNPQAGIAPDEPLWFVVEVKSIDAVPDSSTTTV
                     AAAPEPTTAAP"
     gene            complement(1949..3169)
                     /gene="tyrS"
                     /locus_tag="VFX21_09925"
     CDS             complement(1949..3169)
                     /gene="tyrS"
                     /locus_tag="VFX21_09925"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX5096323.1"
                     /translation="MAASIDEQVDAIFLGAEFGDETLAATMRKEFRDKLVDARARNQP
                     LRVYAGYDPSKPDLHLGHSITMRKLRLFQDFGHDVTFVVGTFTAQVGDASDKLEGRPR
                     LEAERVRAAAESYTEQAFAILDPERTTIRYNDEWLSTLHLPDVIEIASVFTVQQFLAR
                     ETFRKRYDSGDPIRLHEFMYALLQGYDALHLDADVQLGATEQLFNIMAGRRLQDVHGK
                     RGCTPITFPVLVGLDGKSRMSKSAGNYVGLRESPNDQFGKVMSIDDDTMRNWIPLVSS
                     WSPPEVAARLRELDAKSLHPMELKKQLAADIVTLYHGEGAAKEAQGHFEALHQQRDYS
                     AGNAARIVLDTDVLLDAVVKIASESKRGARRLLGEGAVRVNDEIVDDENHRLTTGDLV
                     RVGKRHLYVVEVPA"
     gene            complement(3204..>3479)
                     /locus_tag="VFX21_09930"
     CDS             complement(3204..>3479)
                     /locus_tag="VFX21_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5096324.1"
                     /translation="AARAREIGLAGWVVPHDDLMDEAHRLADRLVRAAPLAARATKEV
                     AVRARHMSTVEAIRFGETMRKVAAATEDAKEGGRAFAEGRRPEWKGR"
BASE COUNT          515 a         1202 c         1203 g          559 t
ORIGIN      
        1 caccaacgcg aacgacacca acccgaacgc tcgccgagat ggccgagctc gccgccggat
       61 tcgcactgcg ggtcgacggt accgaactgg ccggcgccga agtcgtcgac gggcggcgtt
      121 acgtgtgcct cgtccggacc gccagctacg aggcgtggct ggtcgagtgg agtcccgcca
      181 gcatgctggc gccgcacgac cacggcggcg cggccggcgt cctgcacgtc gtcgacggcg
      241 agctggtcga gacataccgc gacgacaaga agacaaagtc gcgtgaacgc aagatagagg
      301 cgggatacac cgtgcgcgtc cctgcgtcgc gcgagcacga agtgtggaat gcaacggccc
      361 gacctgcacg cagcatccac gtgtactcgc caccgggact cgtctctagt gcgttgaccg
      421 cgccagcttc gtagtcttcc gttcgtgccg accatcgccg cgaacgggat cgagctgtac
      481 ttcgagcggc cgggtgccgg cccgcggctg ttgttcctga acggctcggg ctccacgatc
      541 gagaccagcc gcttgttgat ccagccgttc accgaacggt tcgaggtgct cgcgttcgac
      601 cagcggggcc tcggcctgag cgcgatcccg cccggtccgt acgagatggc cgactacgcc
      661 gcggacgcaa tcgggttgct cgacgcggtc gggtggccgt catgtcgcgt cttcggcgtg
      721 agcttcggcg ggatggtcgc gcaggagctc gcggtcaccg cacccgatcg catcgagcgg
      781 ctcgtgctgt cgtgcacgtc accgggcggc gcggcgggtg cgtcgtaccc gttgcacgag
      841 ctcgcggcca tgcctgcgga cgaagcggcc gcgctcggca tgcgcctgct cgacacgcgc
      901 ttcacgccgg agtacctcgc cgccaacgcc ggtgatcgcg gtctcgcgga gatgatggtg
      961 gcccgccgca gcgcggagaa gtccgacgag caaaggcgcg gcgaagcgga gcagatgatg
     1021 gcccgcagcc gtcacgacgt cgtggcgcgg ctcggtgcga tcagcgcgcc gaccctcgtc
     1081 gccgccggcc gctacgacgg catcgcgccg ctgccgaaca gcgaagcgat cgcgtcacgt
     1141 gtgccgaacg cagagttgcg cgtctacgaa ggcgggcacg cgttcttcgc gcaggacgcg
     1201 aaggcgctgc cggagatctt cgacttcctc gcccagtagc actcagggcg cggcggtcgt
     1261 cggctcgggc gcggccgcca ccgtggtcgt gctcgagtcg gggacggcat cgatgctctt
     1321 gacctcgacg acgaaccaca gcggttcgtc gggcgcgatg cccgcctgcg gattgcccgc
     1381 gggcccgtac gcctggtcgc cagggatacc gagcaggcga cgaccaccga ccttcatgcc
     1441 gggaatgccg tcggtccagc ccttgatcac accgtccaac gagaacggcg tcggctcgcc
     1501 gcgcgagtac gacgagtcga agatcttgcc ggtcgtgcac gcgactccga tgtagtcggc
     1561 ggtgatcgac gcgccggccg gcaccacttc gccggtgccc tcggtcaggt ccttgatcac
     1621 cagcgacgtc ggtggcggac cgacgacgac gtcgacggcc ggcgcacctt cgggaagcgg
     1681 gtcgaccacg gcgacgcagg gcttgcccgc gacggatccg gccacggttg tcggggtgtc
     1741 cgttgcgctg acgctgtcgt cgtcatcgtc gaacggcttg gcgacgaaga agaacgcgac
     1801 cgcggcggca atgaccaatc caccgatcgc gatggcccag cgccggttgc gggcgtgccg
     1861 cacccggtac tcgcgctcgg cttcgcggtt ctccgcgttc aggcgccgcc gttcccgatt
     1921 ccgagagctc acggccgggg aacgtacctc atgccggcac ctcgaccaca tagaggtggc
     1981 gcttgccaac ccgcacgaga tcgcccgtcg tgagccggtg gttctcgtcg tcgacgatct
     2041 cgtcgttgac ccgcaccgcc ccctcgccga gcagccggcg cgcgccccgc ttcgactcgc
     2101 tcgcgatctt caccaccgcg tcgagcagca cgtcggtgtc gagcacgatg cgagcggcgt
     2161 tgccggcgct gtagtcgcgc tgttggtgca acgcctcgaa gtgcccctgc gcctccttgg
     2221 ccgcgccctc accgtggtac agcgtgacga tgtcggccgc gagctgcttc ttcagctcca
     2281 tcggatgcag cgacttcgcg tcgagctcgc gcagccgcgc ggcgacctcg ggtggcgacc
     2341 acgagctcac gagcgggatc cagttgcgca tcgtgtcgtc gtcgatgctc atgaccttgc
     2401 cgaactgatc gttcggagac tcgcgcaacc cgacgtagtt ccccgcgctc ttcgacatgc
     2461 gcgacttgcc gtcgagcccg acgaggaccg gaaacgtgat cggcgtgcag ccgcgcttcc
     2521 cgtgcacgtc ttgcagccgg cggccggcca tgatgttgaa cagctgctcc gtcgcgccga
     2581 gctgtacgtc ggcgtcgaga tgcagcgcgt cgtatccctg cagcagcgcg tacatgaact
     2641 cgtgcagacg gatcgggtcg ccggagtcgt aacgcttgcg gaacgtctcg cgcgcgagga
     2701 actgctgcac cgtgaacacc gacgcgatct cgatcacgtc gggcaggtgg agcgtgctca
     2761 gccactcgtc gttgtagcga atggtcgtgc gttcgggatc gaggatcgcg aaggcctgct
     2821 cggtgtacga ctcggccgcc gcgcgcacgc gctcggcctc cagccggggg cggccctcga
     2881 gcttgtcgct cgcgtcaccg acctgcgcgg tgaacgtgcc gacgacgaag gtgacgtcgt
     2941 ggccgaagtc ctggaacagg cgcagcttgc gcatcgtgat cgagtgcccg aggtggaggt
     3001 cgggcttcga ggggtcgtaa ccggcgtaga cgcgcagcgg ctggttgcgc gcgcgcgcgt
     3061 cgaccagctt gtcgcggaac tccttgcgca tcgtcgccgc cagcgtctcg tcaccgaatt
     3121 cggcaccgag gaagatggcg tcgacctgtt cgtcgatcga ggccgccata gatgttcgag
     3181 gttacttggc ggccggagcg gccctaccgg cctttccatt cgggacggcg gccttccgcg
     3241 aacgcgcggc cgccttcctt ggcgtcttcg gtcgcggccg cgaccttgcg catcgtctcc
     3301 ccgaagcgga tcgcttcgac ggtcgacatg tgccgcgcac gcacggcgac ttccttcgtc
     3361 gcgcgcgcgg cgagcggggc ggcgcggacg agacggtcgg cgaggcggtg ggcttcgtcc
     3421 atgagatcgt catgcggcac gacccaaccc gcgaggccga tctccctcgc gcgggcggc
//
LOCUS       DASUGA010000505         3168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_433631, whole genome shotgun sequence.
ACCESSION   DASUGA010000505 DASUGA010000000
VERSION     DASUGA010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433631"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VFX21_09935"
     CDS             <1..202
                     /locus_tag="VFX21_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009720360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5096325.1"
                     /translation="DEFFERDGWRIERYCPHRQADLTRFGDIDDGVLTCALHHWQFDL
                     ATGRCLTSDGADKHLRCSRIDP"
     gene            213..665
                     /locus_tag="VFX21_09940"
     CDS             213..665
                     /locus_tag="VFX21_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5096326.1"
                     /translation="MNTAFNHLGQCVTDLERSKRFYMELFGFTLEREINPPDESSAQL
                     LGLAPPLGMTACYLVRDGLVLELLHFAAPGQTQPYRARSMNEPGLTHISLSVDDVDDV
                     VRRVADYGGVALPATNIGAATFVRDPDGQLVELLPMEYRRRLDEAGPR"
     gene            complement(670..1173)
                     /locus_tag="VFX21_09945"
     CDS             complement(670..1173)
                     /locus_tag="VFX21_09945"
                     /inference="COORDINATES: protein motif:HMM:NF023430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 family protein"
                     /protein_id="HEX5096327.1"
                     /translation="MPGAEEQGGDPPAMISGPIWARRYTTRSPRLCARPGCQSPAAAT
                     LRFHSTRREARLVDVDADGRPCSGDLCERHACVLSLPRGWRLCDHRTLVAPEPAAELV
                     IKFESEPEPEPEGVREPESEPEPEPERVLEPLMGSEPPADPDALSAMLDATTPLLRRA
                     FRNAGPT"
     gene            complement(1224..1556)
                     /locus_tag="VFX21_09950"
     CDS             complement(1224..1556)
                     /locus_tag="VFX21_09950"
                     /inference="COORDINATES: protein motif:HMM:NF023430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 family protein"
                     /protein_id="HEX5096328.1"
                     /translation="MRRQCARASCSAIATATFTFDAEVKTVWLDAPSEGAARAGELCE
                     RHVRALTPPQGWELDDRRAPVRPAQQAVTENSSARANVPDNVSDLRRVLDARTPLLAR
                     AFRAAGTV"
     gene            1672..2415
                     /locus_tag="VFX21_09955"
     CDS             1672..2415
                     /locus_tag="VFX21_09955"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX5096329.1"
                     /translation="MALALGAHLEVATDGDPAPETHPAVRAFRHAGGRGPISVHATFP
                     GGRGMGYSGAARVGGVLAACAQQGRAVWEVRDDVLRVATELEGHADNVAASLVGGIVA
                     VAGGRVVRVPVAREVAVVVWVPDRETPTSSARRLLPDHVSLDDAVFNIGRTALLVAAL
                     AAGEIDVLRTATEDRLHQDRRLSRARDSRGAIEAAVHAGAWCAWLSGSGPSVAALADP
                     ARAADIAAALPASGRARVLAVDDEGATVT"
     gene            2412..>3168
                     /locus_tag="VFX21_09960"
     CDS             2412..>3168
                     /locus_tag="VFX21_09960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008204667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5096330.1"
                     /translation="MRIEGAHVVVTGAASGIGAALARRFAAEGARAVVVADIQDTPLR
                     DVAGNIGAMAQRCDVTKESDIVALVDAAETANGPIDLFCSNAGIAMLGGEQMSNDEWR
                     LSIDVNVMSHVWAARALVPRMLERGRGYLLQTASAAGLLTQIGSAPYSVTKHAVVAFA
                     EWLTITYGERGIKVSVLAPQAVRTAMTAGIPGGGVAGVDGMIEPDDVADAVVAGLDAE
                     QFLILPHPVVLEYFRRKADDYDRWLSGMRRLQAR"
BASE COUNT          425 a         1093 c         1176 g          474 t
ORIGIN      
        1 cgacgagttc ttcgagcgcg atggttggcg tatcgagcgg tactgcccgc atcggcaggc
       61 cgacctcacg cgcttcggcg acatcgacga cggtgtgctg acgtgcgcgc tgcaccactg
      121 gcagttcgac ctggccacgg gccggtgcct gacgagcgac ggtgccgaca agcacctgcg
      181 ctgctcgcgc atcgacccgt aacctgccct ctgtgaacac cgccttcaac cacctcgggc
      241 agtgcgtgac tgatctcgaa cggtcgaagc ggttctacat ggagctgttc gggttcacgc
      301 tcgaacgcga gatcaatcca ccggacgagt cgtccgcgca gctgctcggt ctcgcgccgc
      361 cgctcggcat gaccgcgtgc tatctcgtgc gcgacggact ggtgctcgag ctgttgcact
      421 tcgcggcacc cggccagacg cagccgtacc gcgcccgcag catgaacgag ccgggcctga
      481 cgcacatctc gttgagcgtc gacgacgtcg acgacgtcgt gcggcgggtc gccgactacg
      541 gaggcgttgc gttacctgcg accaacatcg gcgcggcgac gttcgtgcgc gatcccgacg
      601 ggcagctcgt cgagctgttg ccgatggagt accgccgccg cttggacgaa gccgggccgc
      661 gctagcgggt caggtcggcc cggcgttgcg gaaggcgcgg cgcagcagcg gcgtggtggc
      721 gtcgagcatc gccgacagcg cgtccgggtc ggcgggcggc tcggagccca tcagcggttc
      781 gagcacgcgc tcgggctcgg gttccggttc cgattccggc tctcgtacgc cttcgggctc
      841 gggctcgggc tccgactcga atttgatcac cagctcggcc gcgggctcgg gcgcgacgag
      901 ggtgcggtgg tcgcacagcc gccagccccg gggcaacgac aacacgcacg cgtgccgctc
      961 gcagaggtcg ccggagcagg gccggccgtc ggcgtcgacg tcgacgaggc gcgcctcgcg
     1021 gcgcgtggag tggaatcgca acgtggccgc ggcgggcgac tggcaaccgg gacgcgcaca
     1081 gaggcgcggg gatcgcgtgg tgtagcgccg ggcccaaatc ggcccagaga tcattgccgg
     1141 cggatcaccc ccctgctcct cggcacccgg catgcatcct tttccccggt gacgccagtt
     1201 tgacgcaggt ggaggggaga acttcaaacc gtgccggccg cccggaacgc gcgagcgagc
     1261 agcggcgtgc gggcgtcgag cacgcgtcgc aggtcggaga cgttgtcggg gacgtttgcc
     1321 cgggccgaag aattctcggt gacggcttgt tgcgcgggcc ggaccggggc gcggcggtcg
     1381 tcgagctccc aaccctgagg gggcgtcagc gcgcggacgt gccgctcgca cagctccccg
     1441 gcccgggccg ccccctccga cggcgcatcg agccagaccg tcttcacctc cgcgtcgaag
     1501 gtgaacgtag cggtcgcgat cgcgctgcag ctcgcgcgtg cacactgtcg tcgcacgaca
     1561 ttgacggtac tgcctcgcgc gcgtcaccac gtgtcatcct gggatgtgcg cgaacggttt
     1621 cgggtgcggg taccggcgtc gtcggcgaat ctcgggcccg cgtttgatgc gatggcactc
     1681 gccctcggtg cccacctcga ggtcgcgacc gacggcgacc ccgcgcccga gacacacccg
     1741 gcagtacgag cctttcgcca cgccggcggc cgcgggccga tctcggtgca cgcgacgttc
     1801 cccggcgggc gggggatggg ttactccggc gcggcccgcg tcggcggcgt gcttgcggcc
     1861 tgcgcgcagc aggggcgggc ggtgtgggaa gtgcgcgacg acgtgctgcg ggtcgcgacc
     1921 gagctcgaag ggcacgccga caacgtcgcg gcgtcgttgg tcggcggcat cgtcgcggtc
     1981 gcgggcggcc gcgtcgtgcg ggtaccggtt gcgcgcgagg tcgcagtcgt ggtgtgggtt
     2041 cccgatcgcg agacgccgac gagctccgcg cggcggctct tgcccgacca cgtgtcgctc
     2101 gacgacgcgg tcttcaacat cgggcgtacc gcgctcttgg tcgccgcgct cgcggccggt
     2161 gagatcgacg tgttgcgcac cgcgaccgag gaccgcctgc accaggaccg ccgcctgtcg
     2221 cgggcgcgcg attcgcgggg cgcgatcgaa gcggccgtgc acgcgggcgc atggtgtgcg
     2281 tggctgtcgg gttcggggcc gtcggtcgcc gcgctcgcgg atccggcgcg ggcggccgac
     2341 atcgcggctg ccctcccggc gagcggccgg gcgcgcgtgc tcgcagtcga cgacgaagga
     2401 gcaacggtga catgaggatc gaaggcgcac acgtcgtcgt caccggcgcc gcgagcggca
     2461 ttggtgccgc gctcgcgcgg cggttcgcgg ccgaaggcgc ccgcgccgtc gtcgtcgccg
     2521 acatccagga cacgccactg cgtgacgtcg ccggcaacat cggcgcgatg gcgcagcgct
     2581 gcgacgtcac caaggagtcc gacatcgtcg cgctcgtcga cgcggcggag accgccaacg
     2641 gaccgatcga cttgttctgc tcgaacgccg ggatcgcgat gctcggcggt gagcagatgt
     2701 cgaacgacga gtggcggctc agcatcgacg tcaacgtcat gtcgcacgtc tgggccgcgc
     2761 gcgcgttggt gccgcgcatg ttggagcgcg gccgcgggta cttgctgcag accgcgtcgg
     2821 ccgccgggtt gctgacgcag atcggctccg cgccgtactc cgtcacgaag cacgccgtcg
     2881 tcgcgttcgc ggagtggttg acgatcacct acggcgagcg cggcatcaag gtctcggtgc
     2941 tcgcgcccca ggcggttcgc accgcgatga cggccggcat tcccggcggc ggtgtcgcgg
     3001 gggtcgacgg catgatcgaa ccggacgacg tcgcggacgc ggtcgtcgcc gggctcgacg
     3061 ccgagcagtt cttgatcctg ccgcacccgg tcgtgctcga gtacttccgc cgcaaggccg
     3121 acgactacga ccgctggctc tcggggatgc gtcgcctgca agcgcggt
//
LOCUS       DASUGA010000506         2204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1127708, whole genome shotgun sequence.
ACCESSION   DASUGA010000506 DASUGA010000000
VERSION     DASUGA010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127708"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VFX21_09965"
     CDS             <1..802
                     /locus_tag="VFX21_09965"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5096331.1"
                     /translation="RAFVLDGALDPARGFEEVTREQAMGFDSALNAFFDDCAKNGCGF
                     GGDDARSAYEKLAAQIDAETLPAEVGGEERELGPGEFDLGVATALYAGKSGWEVLADG
                     LRDAASGDGSILVSLSDTYTGRETDAEYDNSFEAFLGIGCLDAPAPTIDELGKMADDI
                     AADAPYFGEATAWLSAPCSLWPVPAELTPGPVTAAGAPPIVVIGTSNDPATPLEWAES
                     LADELQSGHLITFQGEGHTAYAQGNGCVDDAVDDYLVDLQVPEDGLKC"
     gene            complement(846..2009)
                     /locus_tag="VFX21_09970"
     CDS             complement(846..2009)
                     /locus_tag="VFX21_09970"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX5096332.1"
                     /translation="MLGEVGKVRSLLEALARDFDASILHGRDSLELVRELGAIQCLLD
                     GLKARAAKRVEETCAYMAVHDADAKYTVARAFRIEPSEAQRTINLAERLESLTVVDAA
                     VRAGEVSGREAQLIVEAAQHNPDAVPQLLTSAGSGLASLKDACVAARAVVEDPDARSK
                     RQHRQRSFRMWTDSDGMMVGRFALTPEVGGQIKAIVDKGVQQIFRKRRAGEEHEPHEA
                     YAADVLANAFLHPTEVPAAKVTTHLVMDHSILALGDQLPGAKCEIPGVGPVNAQWARD
                     LLGESFLTFVIKKGKDITTVAHVGRHIPAELRTALVVSGRECDVEGCHNRGYLEIDHS
                     HPFAKGGVTSWQNLRWLCYLHHKRKTQGHELPRKRSQSRARRPALVSAGSRDP"
BASE COUNT          374 a          726 c          727 g          377 t
ORIGIN      
        1 gcgtgcgttc gtgctcgacg gcgcgctcga tcccgcgcgc ggcttcgagg aagtcacgcg
       61 cgagcaggcg atgggcttcg actccgcgct gaatgcgttc ttcgacgact gcgcgaagaa
      121 cggttgcggc ttcggtggcg acgatgcgcg gtccgcgtac gagaagctcg ccgcgcagat
      181 cgacgcggag acgctgcccg ccgaagtcgg aggcgaggaa cgggagctcg gtcccggcga
      241 gttcgacctg ggcgtggcaa ccgcgttgta cgccggcaag tcgggctggg aagtgctcgc
      301 cgacggactc cgggacgcgg cgagcggcga cggctcgatc ctcgtctcgc tctcggacac
      361 ctacaccggt cgcgaaacgg atgctgaata cgacaactcg ttcgaggcgt tcctcggcat
      421 cgggtgtctc gacgcgcccg cgccgacgat cgacgagctc ggaaagatgg ccgacgacat
      481 cgcggccgac gcgccctact tcggcgaagc gaccgcatgg ctgtcggcac cgtgctcgct
      541 gtggccggtg ccggccgagc tgacgccggg cccggtcacg gccgcgggcg cgccgccgat
      601 cgtcgtgatc ggcaccagca acgatccggc gacgccgctc gagtgggcgg agtcgctcgc
      661 ggacgagctc cagtcggggc acctcatcac gttccaaggc gagggccaca ctgcgtacgc
      721 ccaaggcaac ggctgcgtcg acgacgcggt cgatgactac ctcgtcgact tgcaggtccc
      781 cgaagacggc ctgaagtgct gatcaattgt caaagagcag ttggggaaat gcgcgcgcgc
      841 gtacgtcagg ggtcgcgcga gcccgctgag accagcgccg gtcgtcgggc gcgcgactgc
      901 gatctcttac ggggaagttc gtggccctgg gtcttgcgtt tgtggtggag gtagcagagc
      961 catcggaggt tctgccatga cgtgacaccg cccttcgcaa acggatgcga gtgatcgatc
     1021 tcgagatagc cgcggttgtg gcatccctcg acgtcgcact cgcgaccact cacgaccaat
     1081 gcagtgcgca gctccgcggg aatgtgccga ccgacgtggg cgacggtggt gatgtccttg
     1141 cccttcttga tgacgaaggt caagaacgac tcgcccagta ggtcacgcgc ccattgcgcg
     1201 ttcaccggtc cgacgccggg gatctcgcac ttcgcgcccg gcaactgatc accgagcgcc
     1261 aggatgctgt ggtccatcac gagatgagtg gtgaccttgg ccgcgggcac ttctgtcgga
     1321 tgtaagaacg cgttggcgag cacgtcggcg gcataggcct cgtgcggctc gtgttcttca
     1381 ccggcccggc gcttgcggaa gatctgctgc acgcctttgt cgacgatggc tttgatctgc
     1441 ccgccgacct cgggagtgag cgcaaaccga ccgaccatca tcccgtcgct gtcggtccac
     1501 atccggaacg accgctgccg atgttggcgc ttcgaccgcg cgtccgggtc ttcaacgacg
     1561 gcgcgcgccg cgacgcaggc gtccttcaac gaagcgagcc cggagcccgc cgacgtcaag
     1621 agttggggga cggcgtcggg attgtgctga gcggcttcca cgatgagctg cgcctcgcgg
     1681 ccggatacct cgccggctcg cacggcggca tcgacgaccg tcagcgactc gagtcgttcc
     1741 gcgaggttga tcgtgcgctg cgcctcggac ggctcgatgc ggaacgcgcg cgccacggtg
     1801 tacttcgcat cagcatcgtg caccgccatg tacgcgcacg tctcttcaac ccgcttcgcg
     1861 gcacgcgcct tcaacccatc cagcaagcac tggattgcgc caagctcccg aaccaactcc
     1921 aacgagtctc ggccgtgcaa gatcgaggcg tcgaagtcgc gagcgagcgc ttcgagcagc
     1981 gaacgaacct tccccacttc gccgagcatg cgatcattgt atcgaacgta cgttcgatcg
     2041 caagcacttt cttcaggaaa tatctgcctg cggcccgacg cgtgacgtca cacgacccga
     2101 cgctctcctc gttgcgatca ttgtcgtttg cgcggcaact acttcaaatc caacgacaac
     2161 atgcggatcg cgttgccgcg gacgatcttg tagacgacgt cggc
//
LOCUS       DASUGA010000507         4696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1938290, whole genome shotgun sequence.
ACCESSION   DASUGA010000507 DASUGA010000000
VERSION     DASUGA010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1938290"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFX21_09975"
     CDS             complement(<1..188)
                     /locus_tag="VFX21_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096333.1"
                     /translation="MTIAAVPPPGIDVARVAPAGETAAGGFDELLAGAQTEAPDETRP
                     GAGAKRPVRHAPRDAAKT"
     gene            complement(185..631)
                     /locus_tag="VFX21_09980"
     CDS             complement(185..631)
                     /locus_tag="VFX21_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096334.1"
                     /translation="MKKFKFRLASVQKVRQLEEERARIRLMEANAEARETAARVEARL
                     HAYVSNPRPGEPMSYEAFESERFKLEALATAVESARIAHREALDIVDMRRVEWLEAKQ
                     RVAALERLEVRRREEHAIDLRRAEDRLIDDLVVARHRLRNNQGVGQ"
     gene            complement(628..1983)
                     /locus_tag="VFX21_09985"
     CDS             complement(628..1983)
                     /locus_tag="VFX21_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HEX5096335.1"
                     /translation="MSTLLESVVRAAQPRVQGRVRRVVGLNVEIGGLQLPIGSAVTIH
                     GAAGPVVAEVVAVVDDGLVCLPLGELRGVRAGDFAETSTGAADVPVGYELLGRVIDGL
                     GHPIDDCPSVRALPRVSVDMHAPHPLHRKRIDTQLPLGVRALDTLTPCGRGQRMGIFA
                     GSGVGKSSLLSMITRGTEAPVTVLALIGERGREVREFIENDLGPEGLARSVVVVSTSD
                     TPALVRLRAAFTATRIAEWFRDQGLDVLLLMDSLTRFSMAQREVGLAAGEPPATRGYP
                     PSVFALMPRLLERAGASDQGSITGLYTVLIEGDDLMDPIGDAARSILDGHIVLSRSLA
                     TAGHFPSIDILESISRVTPAITTRDQRDAGSEMRRLLAAYRDAKDLIEIGAYVPGTNP
                     TVDRAVALREATDSFLRQDLHESTPASDAWDALVRLVGRADAAEPRTAQRGSGVNGGT
                     S"
     gene            complement(1980..2606)
                     /locus_tag="VFX21_09990"
     CDS             complement(1980..2606)
                     /locus_tag="VFX21_09990"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HEX5096336.1"
                     /translation="MAQRGRILRGADVAVGPADLSTITASVARDLVVDPRLVEDATRD
                     GFHNGYQVGYDTGFAEALAEGRRQNEQLVIRLQQVIVQLASASDQLFTREATARADLA
                     DEVVAAGFHLAEVLLGHELAHTETRGRDAIARALDFAPETGHVTARCNPGDLASVGDT
                     ESLAPGRALTVVADPSIAPGDCVVDVAACRVDARLRTALDRVEEVLTR"
     gene            complement(2606..3613)
                     /gene="fliG"
                     /locus_tag="VFX21_09995"
     CDS             complement(2606..3613)
                     /gene="fliG"
                     /locus_tag="VFX21_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEX5096337.1"
                     /translation="MAQAKFNGREKAAILIMQMGKEKAAPVLRSMRELEVSQIMGEVA
                     RLHQLEAGEIEEVLNEFRETFVARAHVAQGGYETARELLEGTFGPQRADEILDNLGVT
                     LVSAPFEFLRRADTRQVLTYLQEEHPQTIALVLAHMHPDAAAMVLSALPDEQQAEVAM
                     RIAKMDRTSPDVVEQVEAILERKLSTVIQQSDFAQAGGLQTLVDILNSSDRATERIIL
                     DGLTGSDAELADEVRNRMFVFEDVIGLDDRAIQLVLRSVDAKELSVALKGVDAKVRDK
                     ILKNMSERAAANLVEEIQLLGPVKLKTVEEAQNSIVRVIRTLEEAGQIDMSRGGEELV
                     N"
     gene            complement(3620..>4696)
                     /locus_tag="VFX21_10000"
     CDS             complement(3620..>4696)
                     /locus_tag="VFX21_10000"
                     /inference="COORDINATES: protein motif:HMM:NF019944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HEX5096338.1"
                     /translation="VDTGGTKLSNEAVQSIVNIVASSVADMTPESVTLTDANMNLLHA
                     AGQSLTGGSGDTMEAKAAFEAQMAKSLTDFIAASLGPGHARVDVEAQLDMSSGETHSQ
                     EFTQPTGGQTPTQETTSSENFSGPGQDAAGIVGPDGTPSTPNSTPVSYTKTEADRQLA
                     VNMIEQTKKEAPGTLERLSVSVLMDSEKVAAGDQTAWETALSTAAGIDTARADAITVT
                     RMPFDTEAQKAATENLKAASSAASSNAMLDMIRYFVTFLIVGLVLFLAWRAIKKAEVN
                     RVPIRVPLDLAQLEAGERRMEPAMAGVEQSLRLPAAAAPAERAAPSLEAANVPVEEEI
                     AGIIEQQPDEVALTLRSWLADRRG"
BASE COUNT          657 a         1611 c         1633 g          795 t
ORIGIN      
        1 tgggtcttcg ccgcgtcgcg cggcgcatgc cgcacgggac gcttcgcccc cgcgccgggc
       61 cgcgtctcgt cgggtgcctc ggtctgcgca cccgcgagca gctcgtcgaa gccacccgcg
      121 gcggtctcgc cggccggcgc aacgcgcgcg acgtcgatcc ccggcggcgg aacggcggcg
      181 atcgtcattg accaactcct tggttgttgc gcaaacggtg acgggcgacc acgaggtcgt
      241 cgatgaggcg gtcttcggcg cgccgcaagt cgatggcgtg ctcttcgcga cgccgcactt
      301 cgagccgctc cagcgcggca acgcgctgct tggcctcgag ccactcgaca cgtcgcatgt
      361 cgacgatgtc gagcgcttca cggtgcgcga tgcgcgccga ctcgacggcc gtcgcgagcg
      421 cttcgagctt gaaccgttcg ctctcgaacg cctcgtagct catcggttcg ccgggacgag
      481 ggttgctgac gtaggcgtgc agccgggcct cgacgcgcgc cgcggtctct cgcgcttcgg
      541 cgttggcctc catgagccgg atccgggcgc gctcttcctc gagctggcgg accttctgca
      601 ccgatgccaa acggaatttg aacttcttca actggtgcct ccattcacac cgctccctcg
      661 ctgcgccgtc cgtggctccg ctgcgtccgc acggccgacc agtcgtacga gtgcgtccca
      721 tgcgtccgat gcgggagtcg actcgtgcag gtcctggcgc aggaagctgt ccgtcgcttc
      781 tcggagtgcc acggcgcggt cgacggtcgg gttggtgccc ggaacgtacg cgccgatttc
      841 gatgagatcc ttggcgtcgc ggtacgcagc gagcaggcgg cgcatctcgc tgccggcgtc
      901 ccgctggtcg cgcgtcgtga tcgcgggcgt gacccgggag atcgactcga ggatgtcgat
      961 gctcgggaag tgtcccgccg tcgcgagcga ccgcgagagc acgatgtgac cgtcgaggat
     1021 cgaccgcgcc gcgtcgccga tcgggtccat gaggtcgtcg ccctcgatga ggacggtgta
     1081 gagcccggtg atcgatccct ggtccgacgc accggcccgt tccagcaggc gcggcatcaa
     1141 cgcgaacacc gacggcgggt agccacgcgt cgcgggcggt tcgccggccg cgagcccgac
     1201 ttcgcgctgc gccatggaga accgggtgag gctgtccatc aacaacaaca cgtcgagacc
     1261 ctgatcgcgg aaccactcgg cgatgcgtgt cgcggtgaac gcggcgcgca agcgcacgag
     1321 ggccggcgtg tcggaggtcg aaacgacgac gaccgagcgg gcgagccctt cgggtccgag
     1381 gtcgttctcg atgaactccc gtacctcacg accgcgttct ccgatgagcg cgagcaccgt
     1441 gaccggcgcc tcggtgccgc gcgtgatcat ggacagcaag ctcgacttgc cgacaccgga
     1501 tcccgcgaag atgcccatgc gctgcccgcg gccacaaggc gtcagcgtgt cgagcgcgcg
     1561 cacgccgagt gggagctgcg tgtcgatgcg cttgcggtgc agcgggtgcg gcgcgtgcat
     1621 gtcgaccgac acgcgtggca gcgcgcgcac cgacggacag tcgtcgatcg ggtggcccag
     1681 gccgtcgatg acgcggccca acaactcgta cccgacgggc acgtcggccg cgccggtgga
     1741 ggtctcggcg aagtcaccgg cgcgcacgcc gcgcagttca cccagcggca ggcacacgag
     1801 cccgtcgtcg acgaccgcga cgacttcggc gacgaccggt ccggccgcgc cgtggatggt
     1861 gaccgcgctg ccgatcggca gttggaggcc gccgatctcg acgttgagac cgacgacgcg
     1921 gcgcacgcga ccttgcacac gcggttgcgc ggcccggacg acgctctcga gcagcgtgct
     1981 catcgcgtga gcacctcctc gacacgatcg agcgcggtgc gcagacgagc gtcgacgcgg
     2041 caggcggcga cgtcgacgac gcagtcgccg ggcgcgatgc tcgggtcggc gacgacggtc
     2101 agcgcgcggc ccggtgccag cgactccgtg tcgcccaccg aggccaggtc cccggggttg
     2161 caccgcgcgg tgacgtggcc ggtctcgggt gcgaagtcga gcgcgcgcgc gattgcatcg
     2221 cggccgcgcg tctcggtgtg tgcgagctcg tggcccagca acacttcggc gaggtggaag
     2281 cccgcggcga cgacctcgtc ggcgagatcg gcgcgcgcag ttgcttcacg ggtgaacagc
     2341 tgatccgacg cgctcgcgag ctgcacgatc acctgctgga gccgaatgac caactgctcg
     2401 ttctggcgcc ggccttctgc cagcgcttcc gcgaagccgg tgtcgtaacc gacctggtac
     2461 ccgttgtgga agccgtcgcg cgtcgcgtcc tcgacgaggc gcgggtccac gacgagatcg
     2521 cgcgcgacgc tcgcggtgat cgtcgacaag tcggcgggtc cgactgcgac gtcggcgccc
     2581 cgaaggatgc gtccgcgctg cgccatcagt tcaccaactc ttcgccgccg cgcgacatgt
     2641 cgatctggcc tgcttcttcg agtgtccgga tgacacgcac gatcgagttc tgcgcttctt
     2701 cgaccgtctt caacttgacc gggccgagca gctggatctc ctcgacgagg ttcgcggccg
     2761 cgcgctccga catgttcttg aggatcttgt cgcgcacctt ggcgtcgaca cccttgagcg
     2821 cgacggacag ctccttggcg tccaccgagc gcagcacgag ctggatcgca cggtcgtcga
     2881 ggccgatgac gtcctcgaac acgaacatgc ggttgcgtac ttcgtcggcc agttcggcat
     2941 cggagcccgt caacccgtcg aggatgatgc gctcggtcgc gcgatcggac gagttcaaga
     3001 tgtcgacgag cgtctggagc cccccggcct gcgcgaagtc ggactgctgg atgacggtcg
     3061 agagcttgcg ttcgagaatc gcctcgacct gctcgacgac gtccggcgac gtgcgatcca
     3121 tcttggcgat gcgcatcgcg acctccgcct gctgctcgtc gggcagcgcg ctgagcacca
     3181 tcgccgccgc gtccgggtgc atgtgcgcga gcaccagcgc gatcgtctgc gggtgctctt
     3241 cctggagata ggtgagcacc tggcgagtgt cggcgcggcg caagaattcg aacggtgcgg
     3301 agacgagcgt gacgccgagg ttgtcgagga tctcgtcggc gcgctgggga ccgaacgtgc
     3361 cctccaagag ctcgcgcgca gtttcgtaac cgccctgggc gacgtgggcg cgggcgacga
     3421 acgtctcgcg gaactcgttg aggacttctt cgatctcgcc ggcttcgagc tgatgcagcc
     3481 gggcgacttc gcccatgatc tgcgacactt ccagctcgcg catcgaccgc aggacgggcg
     3541 cggccttctc cttccccatc tgcatgatga ggatggcggc cttctcccgt ccgttgaact
     3601 tcgcctgcgc catcggctac tacccccgac ggtccgcgag ccaggaacgc agcgtgagcg
     3661 cgacctcgtc gggttgctgc tcgatgatgc cggcgatctc ttcttcgacc ggcacgttcg
     3721 cagcttcgag cgacggcgcg gctcgctcgg cgggcgccgc ggcggccggg agtcgcagcg
     3781 actgctcgac acccgccatc gcgggctcca tgcgacgctc ccccgcttcg agctgggcga
     3841 ggtcgagcgg cacgcggatc ggcacgcggt tcacttccgc cttcttgatc gcgcgccagg
     3901 cgaggaagag cacgagcccg acgatgagga acgtgacgaa gtagcggatc atgtcgagca
     3961 ttgcgttgct cgaggccgca ctgctcgcgg ccttgaggtt ctccgtcgcc gccttctgcg
     4021 cttcggtgtc gaagggcatg cgggtgacgg tgatcgcgtc ggcgcgcgcg gtgtcgatgc
     4081 ccgcggcggt cgagagcgcg gtttcccacg cggtctggtc gccggcggcc accttctcgg
     4141 agtccatgag caccgagacc gagagtcgtt cgagggtgcc cggcgcctcc ttcttcgtct
     4201 gttcgatcat gttgaccgcg agctgccggt cggcctcggt cttggtgtag ctgaccggcg
     4261 tggagttcgg cgtcgacggc gtgccgtcgg ggcccacgat gccggccgcg tcctggcccg
     4321 gaccgctgaa gttctcgctg ctcgtggtct cctgcgtcgg cgtctgcccg ccggtcggtt
     4381 gcgtgaattc ctggctgtgc gtctcgccgc tgctcatgtc gagctgcgcc tcgacgtcga
     4441 cgcgcgcgtg accggggccg agcgacgcgg cgatgaagtc ggtcagcgat ttcgccatct
     4501 gcgcttcgaa cgcagccttg gcctccatcg tgtcgccact gccgccggtc aacgactggc
     4561 ccgcggcgtg cagcaagttc atgttggcgt cggtcagcgt caccgactcg ggcgtcatgt
     4621 ccgcgacgct cgacgcgacg atgttgacga tcgactgcac ggcttcgttc gacagcttcg
     4681 tgccaccggt gtcgac
//
LOCUS       DASUGA010000508         2217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1910503, whole genome shotgun sequence.
ACCESSION   DASUGA010000508 DASUGA010000000
VERSION     DASUGA010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1910503"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VFX21_10005"
     CDS             <1..803
                     /locus_tag="VFX21_10005"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5096339.1"
                     /translation="GAANFAGARAISEAYAQALKAKGYDVTFKDNVGTREQLYPLVEK
                     GDIDGFGDFTGSLLTYLKGTPVGDSTETYNLLKEKLNGTGLEATQPAEAQDVNGFYVT
                     KDTADKYGLVTMSDLKKNDVDKQLVFGGPPECQERPLCLGKAEQDTYGLDFKEVKKLD
                     TGGPITAKALKDGTIQVGELFTGSSVIDPDFVLLQDDKKLQPADNPILLVRTPKATAE
                     VKEIVDAVNAKMTLEAYNKMSTAITEDKVDPADAAAEFLKDAGITTSS"
     gene            complement(831..1448)
                     /locus_tag="VFX21_10010"
     CDS             complement(831..1448)
                     /locus_tag="VFX21_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5096340.1"
                     /translation="MTLTRPAHYETSDVRIDKIVVGGYENNVFILRDKGTGEAVLIDA
                     ANEHDLLLQVSKATGVRRVLTTHGHFDHIQAVTQMRDAGIDVGIATEDAEMLPGYDFI
                     IPDDEEIRVGALKLKTIHTPGHTRGSTCFLLEGHPVLFSGDTLFPGGVGNTTFEGGDF
                     PTIIQSVDRRLFTLPEDTLVLPGHGLDTTIGSERPHLDEWVERGW"
     gene            complement(1458..>2217)
                     /locus_tag="VFX21_10015"
     CDS             complement(1458..>2217)
                     /locus_tag="VFX21_10015"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HEX5096341.1"
                     /translation="GFSAAPPLDLDGLNALYAAWGQNLPFDNVVKRIHMASGSTDPIP
                     NGPPEAFFAQYLRDGTGGTCWPSSGALYALLASIGFPARRSSAAMRDDPNGPNEHTHG
                     TVLVAFDGVEYWTDTAMLTERVFPLRVGAGIDDPVHRIRVEPAGDLWRVWWTHPFTDE
                     MLSCLLLDPDVTQEHYLARYEWSRGHSPFNRFLNATKNVGDTRITLAIGRRFERTPDG
                     VTNTPLEGDERIKVMVEEFGYSEEIATRLPPDDT"
BASE COUNT          411 a          682 c          756 g          368 t
ORIGIN      
        1 tcggcgccgc gaacttcgcc ggtgctcgcg cgatcagcga ggcgtacgcg caggcgctga
       61 aggcgaaggg ctacgacgtg acgttcaaag acaacgtcgg aacccgggaa cagttgtatc
      121 cgttggtcga aaagggcgac atcgacgggt tcggcgactt caccggctcg ttgctcacct
      181 acttgaaggg cacgccggtc ggcgacagca ccgagaccta caacctgctc aaagagaagt
      241 tgaacggcac cgggctcgag gcgacgcagc ccgccgaggc gcaggacgtc aacggcttct
      301 atgtcaccaa ggacacggcc gacaagtacg ggctggtcac gatgtcggac ttgaagaaga
      361 acgacgtcga caagcaactc gtgttcggtg gtccgcccga gtgccaggag cgtccgctct
      421 gcttgggcaa ggcggaacag gacacgtacg gcctcgactt caaggaagtg aagaagctcg
      481 acaccggcgg tccgatcacg gcgaaagcgt tgaaggacgg gacgatccag gtcggtgaac
      541 tgttcaccgg ttcgagcgtg atcgaccctg acttcgtgct gttgcaagac gacaagaagc
      601 tgcaaccggc cgacaacccg atcttgttgg tccgcacgcc gaaggccacg gccgaggtga
      661 aggagatcgt cgacgcggtg aacgcgaaga tgacactcga ggcctacaac aagatgtcga
      721 cggcgattac cgaggacaag gtcgacccgg cagacgcggc ggccgagttc ttgaaggacg
      781 cagggattac gaccagttcg taaagcccga cggtttcggg tcgggcgtgg ctaccagccg
      841 cgctcgaccc actcgtcgag gtgcgggcgc tccgaaccga tcgtggtatc gagcccgtga
      901 ccggggagga cgagcgtgtc ctccggcaac gtgaagaggc gccggtcgac cgactggatg
      961 atcgtcggga agtcgccgcc ttcgaacgtc gtgttgccga cgccgcccgg gaacaacgtg
     1021 tcgccgctga agagcaccgg gtgcccttcg agcaagaagc acgtcgagcc ccgagtgtgg
     1081 ccgggcgtgt ggatcgtctt gagcttcaat gcaccgacgc ggatctcttc gtcgtcaggg
     1141 atgatgaagt cgtagccggg cagcatctcg gcgtcctcgg tcgcgatgcc gacgtcgatt
     1201 ccggcgtcgc gcatctgcgt gaccgcttgg atgtgatcga agtgcccgtg cgtcgtgagg
     1261 acgcgccgca cgccggtcgc cttggagact tggagcaaca ggtcgtgctc gttggcggca
     1321 tcgatgagga ccgcttcacc ggttcccttg tcgcgcagga tgaacacgtt gttctcgtag
     1381 ccaccgacga cgatcttgtc gatccgaacg tcggaagtct catagtgcgc aggccgcgtc
     1441 aacgtcatgt atccagccta ggtgtcgtcg ggcgggagcc gggtcgcgat ctcctcggag
     1501 tagccgaact cttcgaccat cactttgatg cgctcgtcgc cctcgagcgg cgtgttcgtg
     1561 acgccgtcgg gcgtgcgctc gaaccgccgg ccgatggcga gggtgatccg ggtgtcgccc
     1621 acgtttttcg tcgcgttgag aaacctattg aacgggctgt gcccgcgcga ccactcgtac
     1681 cgggcgaggt agtgctcctg tgtgacgtcg gggtcgagca ggaggcagct gagcatttcg
     1741 tccgtgaacg gatgcgtcca ccacacgcgc cacaggtcac cggcgggctc gacgcgaatc
     1801 cggtgaaccg ggtcgtcaat ccccgcgccc acgcgcagcg ggaacactcg ctcagtcaac
     1861 atcgcggtgt cggtccagta ctccacaccg tcgaacgcca ccagcaccgt gccgtgcgtg
     1921 tgctcgttcg gcccattcgg gtcgtcgcgc atcgccgccg acgagcggcg cgccgggaag
     1981 ccgatcgacg cgagcagcgc gtagagcgcg cccgacgagg gccagcaggt gccgccggtt
     2041 ccgtcgcgca ggtactgggc gaagaacgcc tccggcggtc cgttcggaat cgggtcggtc
     2101 gagccactcg ccatgtggat gcgcttcacg acgttgtcga acggcaggtt ctggccccag
     2161 gcggcgtaga gcgcgttgag gccgtcgagg tcgagcggcg gtgccgccga gaaccca
//
LOCUS       DASUGA010000509         2416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1945045, whole genome shotgun sequence.
ACCESSION   DASUGA010000509 DASUGA010000000
VERSION     DASUGA010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1945045"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VFX21_10020"
     CDS             complement(<1..639)
                     /locus_tag="VFX21_10020"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX5096342.1"
                     /translation="MSEVYAISDRYVDELAALDPNTATGAGIAGHDHEMTDLSPAGIE
                     ARVAHTRATIAALDAAPQDTDADRIAAQVMRDRLQNELDFHDAQEDLRPLRIIGSPVQ
                     EVRQVFDLMAFDTDDDWETARQRMEKVPDALAGLEQTLREGMARNVVSARRQALACAQ
                     QAEAWGGEKPFFKNLIAPRGDDAGLAAAAETATTAYAKFGAFLRDEYAPVADP"
     gene            676..2355
                     /gene="ettA"
                     /locus_tag="VFX21_10025"
     CDS             676..2355
                     /gene="ettA"
                     /locus_tag="VFX21_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX5096343.1"
                     /translation="MAHQFILTMRDLRRVHPPDKEVLRGINISMYPDAKIGVLGLNGA
                     GKSTLLKIIAGEDDGYQGECRVTPGFSVGYLPQEPRLDPDKDVLGNVVDGVAHTKGLL
                     DRFEEVCNAMGEPDADFDKLLAEQAELQDKIDAVNGWDLERQLDIAMDALRLPPGDAD
                     VTKLSGGERRRVALCRLLLGKPDLLMLDEPTNHLDAESVAWLERHLQEYHGCVVAVTH
                     DRYFLDNVAGWILELDRGAGIPWEGNYTSWLEQKEQRIAQEEKVDEARRRTLERELEW
                     VRMAPRARQAKGKARITAYDKLVAEAEAAEKRDEKLQISIPPGPRLGDQVVEVEGLSK
                     GYGDKLLIENLSFSLPPGGIVGVIGANGAGKTTLFRMLTGQEQPDAGTMKIGSTVEFA
                     YVDQSRESLDDDKTVYEEITGGVDHLKVGTREVHGRAYVAGFNFKGSDQQKKVGDLSG
                     GERNRLHLAKALLMGGNTLLLDEPTNDLDVDTLRALEEALENFAGCAVIISHDRWFLD
                     RIATHILAFEGDSQVKWFEGNLSAYEADRKRRLGIAADQPHRIKYRSLTRT"
BASE COUNT          444 a          777 c          798 g          397 t
ORIGIN      
        1 ggggtcggca acgggcgcgt actcatcccg caggaacgca ccgaattttg cgtacgccgt
       61 ggttgcggtc tcggctgccg cggcgagccc ggcatcgtcg ccgcgcggcg cgatcaagtt
      121 cttgaagaac ggcttttcac caccccatgc ttcggcctgt tgcgcgcagg cgagcgcttg
      181 gcggcgggcg gagacgacgt tgcgagccat gccttcccgc aacgtctgtt ccaggccggc
      241 gagcgcgtcg ggaaccttct ccatgcgttg acgcgcggtt tcccagtcgt cgtcagtgtc
      301 gaaggccatg agatcgaaga cttggcgtac ttcctgcacc gggcttccga tgatgcgcaa
      361 cggccgcaga tcttcctgcg cgtcgtggaa gtcgagctcg ttttgcaacc gatcacgcat
      421 gacctgcgcc gcgatgcgat ccgcgtcggt gtcctgcggc gccgcatcga gcgcggcgat
      481 ggttgcacga gtgtgcgcca cacgcgcctc gatcccggcg ggggagaggt ccgtcatctc
      541 gtggtcgtgg cccgcgatac cggcaccagt ggcggtgttc ggatcgagcg cggcgagctc
      601 gtcgacgtag cggtcggaaa ttgcgtaaac ctcggacacg gttgctcccg ggtcggccgg
      661 tacggtgggg cgagtatggc tcatcaattc atcctgacga tgcgcgatct gcggcgcgtc
      721 cacccgccgg acaaagaagt cctgcggggc atcaacatct cgatgtaccc cgatgccaag
      781 atcggagtcc tcggcctgaa cggcgcaggc aagtccacgc tgctgaagat cattgcgggc
      841 gaggacgacg gctatcaggg cgaatgccgc gtcacgcccg gattctcggt ggggtacctg
      901 ccccaggaac cgcgcctcga ccccgacaag gacgtgctgg gcaacgtcgt cgacggcgtt
      961 gcccatacca agggcttgct ggaccgcttc gaagaggtct gcaacgccat gggtgagccc
     1021 gacgccgact tcgacaagct gcttgccgag caagccgagc tccaggacaa gatcgacgcg
     1081 gtcaacggct gggatctcga gcgccagctc gacatcgcga tggacgcgct gcggctgccg
     1141 cccggcgacg cggacgtcac caagctcagc ggtggtgagc ggcgccgcgt cgcgctgtgc
     1201 cgcctgctgc tcggcaagcc cgacctcctg atgctcgatg agccgacgaa ccacctcgat
     1261 gccgagtcgg tggcctggct cgaacggcac ctgcaggagt accacgggtg cgtggtcgcg
     1321 gtgacccacg accgctactt cctcgacaac gttgcgggct ggatcctcga gctcgaccgc
     1381 ggcgcgggca ttccctggga ggggaattac acgagctggc tcgagcagaa ggaacagcgc
     1441 atcgcgcagg aagaaaaggt cgacgaggcg cgccggcgca cgctcgaacg agagctcgag
     1501 tgggtgcgca tggcgccgcg cgcgcggcaa gccaagggca aggcccgcat caccgcgtac
     1561 gacaagctcg tcgcggaagc tgaggccgcc gagaagcgcg acgagaaact gcagatctcg
     1621 atcccgcccg gccctcgcct cggcgatcaa gtggtcgagg tcgaagggct gagcaagggc
     1681 tacggcgaca agctgctgat cgagaacctg tcgttctcgc tgccgccggg cggcatcgtc
     1741 ggcgtcatcg gagccaacgg cgcgggtaag accaccttgt tccgcatgct cacgggccag
     1801 gagcaacccg acgcgggcac gatgaagatc ggttccaccg tcgagtttgc ctacgtcgac
     1861 cagtcccgcg agtcgctcga cgacgacaag accgtttacg aagagatcac cggcggcgtc
     1921 gaccacctga aggtcggaac ccgcgaagtg cacggccgcg cgtacgtcgc cggcttcaac
     1981 ttcaagggct ccgaccagca gaagaaggtc ggcgatctct ccggcggtga acggaaccgg
     2041 ctacatctcg ccaaggcatt gctcatgggc ggcaacacgt tgctgctcga cgagccgacg
     2101 aacgacctcg acgtcgacac gttgcgcgcg ctcgaagagg cgctcgaaaa cttcgccggc
     2161 tgcgcggtca tcatctcgca cgaccgctgg ttcctcgacc gcatcgccac gcatattctc
     2221 gctttcgagg gtgattcgca ggtgaaatgg tttgaaggca atctttctgc ctacgaagcg
     2281 gatcggaaac ggcggctggg catcgcggcc gatcaacctc atcggatcaa ataccgttcc
     2341 ttgacccgca cgtagctttg cctcacatta agcaggcggg tcaagaagac ttcctgggcc
     2401 gaatgtcaga cgagtt
//
LOCUS       DASUGA010000510         7483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1997028, whole genome shotgun sequence.
ACCESSION   DASUGA010000510 DASUGA010000000
VERSION     DASUGA010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1997028"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VFX21_10030"
     CDS             complement(<1..456)
                     /locus_tag="VFX21_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5096344.1"
                     /translation="MKFGIFYEHQLPRPWAEDSEYELIQDALEQIEFADRLGIHYAWE
                     VEHHFLEEYSHSSAPEVFLAAASQRTKNIRLGHGIVQTPPGFNHPARIAERISMLDLV
                     SNGRADFGSGESSSEAELGGFMIEPELVGQLHLVERVLQELVLVAGLPRA"
     gene            complement(453..1526)
                     /locus_tag="VFX21_10035"
     CDS             complement(453..1526)
                     /locus_tag="VFX21_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096345.1"
                     /translation="MSNLQLHWTEAELLATDDIAEPLIANGVHCHGGYRADGSYVSPR
                     TKHRVPAIDAWQQSHREQFGTEIIDAPIDMWPPSYPSVAQTKYLIREGVAEPTIATLT
                     RVGTVEGFGGLIRAVHPGDMQRFFDDSIDGTALQHLDRGLFEAHARDEAGWEDEAGHN
                     KMWFAARDIAFDNPVSEDQTQIMLERMGIAQPGGAIPSAADMRASAEAIRRFPELDLG
                     FEMMLRRMISLMFIEVSAFHTFRWAEEVLSDDDLVAGDGEAARIISCVRADETPHVDY
                     LRTALTEVRDRTVVTENGKKIAGADVIQTLWDIALSLSLGENREQFRKTILSEIEHAL
                     AGNTRSAEILEGFHALDPVGVAS"
     gene            1557..2135
                     /locus_tag="VFX21_10040"
     CDS             1557..2135
                     /locus_tag="VFX21_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5096346.1"
                     /translation="MTTVETPRRDPARRAAILNAAATAFAHSGYAATSVEEVATAAGI
                     TKLIVYRHFETKENLYRAVLERVFDRQAELFVEHMVAGMQAGGATRALLLTGRESPDG
                     FRLLWRHAVREPQFADYAHELRDVAVSAARVVVEPFLEPAFVEWAAQTLFDHLVDAVL
                     NWLDYGDPKHDDDFLAREALALQASVRAWANR"
     gene            complement(2205..3149)
                     /locus_tag="VFX21_10045"
     CDS             complement(2205..3149)
                     /locus_tag="VFX21_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX5096347.1"
                     /translation="MSKPEPRYMGGQAVLEGVMMRGETTWAVAVRTPEDGIVVDVRPV
                     AGWSEKYRRIPILRGVMGLGESLALGYRALTWSANKQVPEEEQVSEKAMGFAVAFSMI
                     LFSALFLVLPAVAGSSIHSGEWLPIHLPFQLAEGLVRLGLFIGYLAAISLIKDIRRVF
                     EYHGAEHKTIAAYENGVELTPEAADQFTTAHVRCGTNFLLTVMVVAIVVYTFIPTPNI
                     WAIIVSRVVLIPVIAGLSYEVIRLAAKNMHRAWVRFVMKPGLWLQKLTTREPDLAQLE
                     VAIASLNAVLTAEQKTEVESRAGIRVPQTINPLPAAGN"
     gene            complement(3152..3367)
                     /gene="rpmE"
                     /locus_tag="VFX21_10050"
     CDS             complement(3152..3367)
                     /gene="rpmE"
                     /locus_tag="VFX21_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX5096348.1"
                     /translation="MKTDIHPEYVECKVHCSCGNEFVTRSTVPTLRVELCSECHPFYT
                     GKQKLVDTGGRVERFQRRAAKAKAKKA"
     gene            complement(3412..5175)
                     /gene="rho"
                     /locus_tag="VFX21_10055"
     CDS             complement(3412..5175)
                     /gene="rho"
                     /locus_tag="VFX21_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX5096349.1"
                     /translation="MSDTFDRSQLDGKDREQLSEIASALGVKAISRMRKADLVDAIVT
                     ATKSNGGGTATATKPRKIRSTATGRGGDDIESLAAEQDALATADTKSADDDMAMIRPR
                     RRTAATTTTDAPATEAPSSNGDNASGDASHDDGSSNGDAASASSATQTDDRQSGRQSN
                     QQQQSRDQQSWRDDDGQGNRRRRRRRGRDRDGRPSGGGEQREPRLEDYTGELIDIEGL
                     LDLRDEGYGFLRASGYLPGRNDVYVSASQVRRFSLRKGDYVKGATRPPASNEKYPALL
                     RVDLINNQTPDDARSRVRFEDLTPLFPDSKLRLELDDDPAEITGRIIDLISPIGKGQR
                     GMIVSPPKAGKTTIIKQIVYSIERNNPETHVMVLLVDERPEEVTDMRRHVLRAEVIAS
                     TFDRPSDEHCHVAELTIERAKRLVEQGQDVVIILDGITRLARAYNLAAPASGRIMSGG
                     IDAGALYPPKKFFGAARNVEEGGSLTILATALVETNSKMDEHIFEEFKGTGNMELRLD
                     RRTADRRIYPAIDVEQSSTRHEELLFDRKDLQQVWKLRRVLSALEGAAGLELLIDKLK
                     STKSNGEFLNEIAKAPVGMGT"
     gene            complement(5486..6667)
                     /locus_tag="VFX21_10060"
     CDS             complement(5486..6667)
                     /locus_tag="VFX21_10060"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HEX5096350.1"
                     /translation="MKYVVFVPDGCADVALDELGGKTPLEAAAMPRVAALAQRAEVGR
                     ADVIPPGLPPGSDVGNMAILGFDPREYHTGRAPIEAAAMGVELAPDEVAYRCNLVTLS
                     DDDPPTMIDFAAGHISSEQSHPIVRALEAELGGGREGVKFFPGVEYRHLCVVPRDWSD
                     ADCTPPHDLTGQPAEFPTGSSAPKLRALMDASRDVVRRAARDVGAQATQIWLWGQGVK
                     PALPSFEATYGVRGRMSSAVDLVQGLGVLTGIDVVDVPTATAGFDNDYVAQRDACLAS
                     LRDRDLFVVHIEATDEAGHQGLVDVKVDALERWDRDVIGPIVDALDAAGEPYRILMLP
                     DHATPCTIRTHTSQSVPYLFFDSEAAAAGGAYTEPATAACAPVQAHTLMGRLVGSGIK
                     G"
     gene            6711..>7483
                     /locus_tag="VFX21_10065"
     CDS             6711..>7483
                     /locus_tag="VFX21_10065"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX5096351.1"
                     /translation="MRRLAVVLLLVFLAAACSGDDDDNDTAASTRPSTTTAASTAPVD
                     STSPTTASPPADLEAASLTLAEVASALDSPVALAWRAGDDRMYVAEQSGAVRIVEDGA
                     PLDRPVLEVDVATGGNEQGLLGLTFSADGTKLYVDYTDPDGNTHVDEYTMNGDVADTG
                     SRRELLAVDQPFANHNGGDVIFGPDGMLYITLGDGGSAGDPQQRAQNLDELLGKILRI
                     DPAPASGAEYTIPPDNPFVGRSGARPEIWMWGLRNPWRFS"
BASE COUNT         1142 a         2523 c         2537 g         1281 t
ORIGIN      
        1 cgcccttggg aggccggcga cgagcacaag ctcctgaagg acgcgctcga ccaagtggag
       61 ctggccgaca agctcgggtt cgatcatgaa cccaccgagc tccgcctcgc tcgacgactc
      121 accgctgccg aagtcggcgc ggccgttgct cacgaggtcg agcatcgaga tgcgctcggc
      181 gatgcgcgcg gggtggttga agccgggcgg cgtctgtacg atgccgtggc cgagccggat
      241 gttcttcgtg cgctgcgacg ccgccgcgag gaacacctcg ggcgcgctcg agtgcgagta
      301 ctcctcgagg aagtggtgct cgacctccca cgcgtagtgg atgccgagcc ggtccgcgaa
      361 ctcgatctgt tcgagcgcgt cctggatcag ttcgtactcg gagtcctcgg cccacggccg
      421 cggcagttgg tgttcgtaga agatcccgaa cttcacgacg cgacgccgac cgggtcgagc
      481 gcgtggaagc cctcgaggat ctcggcgctg cgcgtgttgc cggcgagcgc gtgctcgatc
      541 tccgacagga tcgtcttgcg gaactgctcg cggttctcgc cgagcgacag gctgagcgcg
      601 atgtcccaca gagtctggat gacgtcggcg cccgcgatct tcttgccgtt ctcggtgacg
      661 accgtgcggt cgcgcacttc ggtgagcgcg gtgcgcaggt agtcgacgtg cggcgtctcg
      721 tcggcgcgca cgcacgagat gatgcgggcc gcttcgccat cgccggccac gaggtcgtcg
      781 tcgctgagca cttcttcggc ccaccggaac gtgtggaacg ccgacacctc gatgaacatg
      841 agcgagatca tgcggcgcag catcatctcg aacccgaggt cgagctcggg gaagcgccgg
      901 atcgcctcgg ccgacgcacg catgtcggcc gcgcttggga tggctccccc tggctgtgcg
      961 atgcccatgc gctccaacat gatctgcgtc tggtcttcgg aaaccgggtt gtcgaatgcg
     1021 atgtcgcgcg ccgcgaacca catcttgttg tgaccggctt cgtcttccca gcccgcttcg
     1081 tcgcgcgcgt gcgcttcgaa cagaccgcga tcaaggtgct gcaacgcggt gccgtcgatc
     1141 gagtcgtcga agaagcgctg catgtcgccg gggtgtacgg cgcggatcag tccgccgaac
     1201 ccttcgacgg tgccgacacg cgtgagcgtc gcgatcgtcg gctcggcgac gccttcacgg
     1261 atcaggtact tggtctgcgc gacgctcggg tagctcggcg gccacatgtc gatcggcgcg
     1321 tcgatgatct cggtgccgaa ctgctcgcgg tgcgactgct gccacgcgtc gattgcgggc
     1381 acgcggtgct tcgtgcgcgg cgagacgtag ctgccgtcgg cgcggtagcc gccgtgacag
     1441 tgcacgccgt tcgcgatcaa cggctccgcg atgtcgtccg tcgccaacaa ctcggcttcg
     1501 gtccagtgca gctggagatt gctcatggac agaactgtat actctggagt tacaccatga
     1561 caaccgtcga gactccccgc cgcgaccccg cccgccgggc ggccatcctc aatgcggccg
     1621 cgaccgcgtt cgcccactcg ggctacgccg cgacgtccgt cgaggaggtc gcgacggccg
     1681 ccggcatcac aaagctcatc gtgtaccggc acttcgagac caaggagaac ctctaccggg
     1741 cggtgctcga acgggtcttc gatcgccagg cggagctgtt cgtcgagcac atggtggccg
     1801 ggatgcaggc gggcggtgcg acccgggcgc tgctgctcac cggccgcgag tcgcccgacg
     1861 gcttccggct gctgtggcgc cacgcggtcc gcgaaccgca gttcgccgac tacgcgcacg
     1921 agctgcgcga cgtcgccgtc agcgcggcgc gcgtcgtcgt cgagccgttc ctcgagcccg
     1981 cgttcgtcga gtgggccgcg cagacgctgt tcgaccacct cgtcgacgcc gtcctgaact
     2041 ggctcgacta cggcgatccg aagcacgacg acgacttcct cgcgagagaa gccctcgcgc
     2101 tccaagcctc agtccgcgcc tgggccaacc gctaaccctc attctcatga gctatcccgc
     2161 catatgtggc cgtccagctc atgactacga ccgcgaggcg gcgattagtt gccggcggcg
     2221 gggagcgggt tgatcgtctg cgggacgcgg atgccggcgc gggactcgac ctcggtcttt
     2281 tgctccgcgg tcagcactgc gttcagtgac gcgatcgcga cttcgagttg tgcgaggtcg
     2341 ggctcgcggg tcgtcagctt ctgcagccac aggcccggct tcatcacgaa ccgcacccag
     2401 gcgcggtgca tgttcttcgc cgcgagccgg atcacctcgt aggagagccc ggcgatcacg
     2461 gggatcagca cgactcgcga gacgatgatc gcccagatgt tcggcgtcgg gatgaatgtg
     2521 tagacgacga tcgcgacgac catcaccgtc agcaagaagt tcgtgccgca gcgcacgtgc
     2581 gcggtggtga actggtcggc ggcttcgggt gtgagctcca cgccgttctc gtacgcggca
     2641 atcgtcttgt gctcggcgcc gtggtactcg aacacgcgcc ggatgtcttt gatcaacgag
     2701 atcgcggcga ggtacccgat gaacagcccg agccgcacca aaccctccgc gagctggaag
     2761 ggcaagtgga tcggcagcca ctccccgctg tggatcgagc tgcccgcaac cgcgggcaac
     2821 acgaggaaca gcgcggagaa caggatcatc gagaacgcga cggcgaagcc catcgccttc
     2881 tcggagacct gctcctcttc gggcacctgc ttgttcgccg accacgtcag cgcgcggtaa
     2941 cccaacgcga gtgattcgcc gaggcccatg acaccgcgaa ggatggggat gcgccggtac
     3001 ttctcggacc agcccgcgac cgggcggacg tcgacgacga tgccgtcttc aggtgtgcgc
     3061 acggcgacgg cccatgtggt ctcgccgcgc atcatcacac cttcgagcac cgcttgaccg
     3121 cccatgtagc ggggctcggg cttcgacatg gttacgcctt cttggccttc gcctttgcgg
     3181 cgcggcgctg gaagcgctcg acgcgaccgc cggtgtccac gagcttctgc ttgcccgtgt
     3241 agaaggggtg gcactcggag cagagctcca cgcggagggt gggcaccgtc gagcgggtca
     3301 cgaactcgtt gccgcacgag cagtgcacct tgcactccac gtattcgggg tggatgtcgg
     3361 tcttcatgat ggctccagtg taacggccgg aaattgcctg aacaccaggg gtcaggtgcc
     3421 cattcccacg ggcgccttgg cgatctcgtt caggaactca ccgttggact tggtggactt
     3481 cagcttgtcg atcagcagct cgaggccggc cgcaccctcg agggcgctca gcacccggcg
     3541 cagcttccag acctgctgga ggtccttgcg gtcgaagagc agctcttcgt gccgcgtgct
     3601 cgactgctcg acgtcgatcg ccgggtagat gcggcggtcg gcggtacggc ggtcgaggcg
     3661 cagctccatg ttgccggtgc ccttgaactc ttcgaagatg tgttcgtcca tcttcgagtt
     3721 cgtctcgacc agcgcggtcg cgaggatggt cagcgaaccg ccctcctcga cgttgcgggc
     3781 ggcaccgaag aacttcttcg gcgggtacag cgcgccggcg tcgatacccc ccgacatgat
     3841 ccggcccgaa gccggcgccg cgaggttgta cgcacgcgcc agacgcgtga tgccgtcgag
     3901 gatgatgacg acgtcctggc cctgctcgac gagccgcttg gcgcgctcga tggtgagctc
     3961 ggcgacgtgg cagtgctcgt cggagggacg gtcgaacgtc gacgcgatga cctcggctcg
     4021 caacacatgg cggcgcatgt cggtgacttc ttcagggcgc tcgtcgacga gcaacaccat
     4081 gacgtgcgtc tcggggttgt tgcgttcgat cgagtacacg atctgcttga tgatcgtcgt
     4141 cttgcccgcc ttcggcggcg acacgatcat gccgcgctgg cccttcccga tcggcgagat
     4201 caggtcgatg atgcgaccgg tgatctccgc cgggtcgtcg tcgagctcga ggcggagctt
     4261 cgagtcgggg aacagcggcg tgaggtcttc gaatcgcacc cggctgcgcg cgtcgtcggg
     4321 ggtctggttg ttgatgaggt cgacacgcag caacgccgga tacttctcgt tgctggcggg
     4381 cgggcgcgtc gcgcctttga cgtagtcgcc cttgcgcagc gagaaccgcc gcacttgcga
     4441 tgccgacacg tagacgtcgt tgcggccggg cagataaccg ctcgcgcgca agaagccgta
     4501 accctcgtcg cgcaggtcga gcagaccctc gatgtcgatc agctcacccg tgtagtcctc
     4561 gaggcgcggt tcgcgttgct cgccaccgcc gctcgggcgg ccgtcgcgat cgcgaccacg
     4621 acggcggcgc cggcgccggt tgccctggcc gtcgtcgtcg cgccagctct gctggtcgcg
     4681 cgactgctgt tgttggttgc tctgacgccc cgactgccgg tcatcggttt gcgtcgcgct
     4741 cgaagcggac gccgcgtcgc cgttggacga gccgtcgtcg tgcgacgcgt cgccggacgc
     4801 gttgtcaccg ttcgacgacg gcgcttcggt cgcgggcgcg tccgtcgtgg tcgtggccgc
     4861 ggtgcggcgg cgcgggcgga tcatggccat gtcgtcgtcg gccgacttcg tgtccgcggt
     4921 ggcgagtgcg tcctgctcgg ccgccagcga ctcgatgtcg tcaccgccgc ggccggtcgc
     4981 cgtcgatcgg atcttgcgcg gcttcgtcgc cgtcgcggtg ccgccgccgt tgctcttcgt
     5041 cgccgtgacg atggcgtcga cgaggtcggc cttgcgcatc cgggagatcg ccttgactcc
     5101 gagcgcagac gcgatctcgg agagctgctc ccggtccttg ccgtccagtt gcgaacggtc
     5161 gaaggtgtca ctcatgtttc gctcctcccg gccccgtcga atgcgacgtg gtcgggtcgg
     5221 tggttgggga tgcaaagaga aagttgccaa accgttcggt cgggggaatc tgaccgcgcc
     5281 cagcccgaac gggagccctc ggaggagcgc aactcaaaca gaccggcaac gagaagaaga
     5341 aagaagaacg agcagcaaac cggactggct gaggtgctcc gcccctctcc ggaaccggca
     5401 gttccgggcg aggtctcgaa ggatcgttcc taccttatac cccgatcgga tcgcggcgca
     5461 ggttccttga ggcaaacgcc gtcgatcagc ccttgattcc cgacccgacc agtcttccca
     5521 tcagcgtgtg ggcttgaacc ggggcgcagg cggcggtggc cggctcggtg tacgcgcctc
     5581 ccgcggcggc cgcttcggag tcgaagaaca ggtacggcac cgactgggat gtgtgcgtgc
     5641 ggatcgtgca cggtgtcgcg tggtcgggga gcatcaaaat gcggtacggc tcccccgccg
     5701 cgtcgagcgc gtcgacgatc ggaccgatga cgtcgcgatc ccagcgctcg agtgcgtcga
     5761 ccttcacgtc gacgagtcct tggtgtccgg cttcgtcggt cgcttcgatg tgcacgacga
     5821 acaggtcgcg atcgcgcaac gacgcgaggc acgcgtcgcg ttgcgcgacg tagtcgttgt
     5881 cgaaacctgc ggtcgcggtt gggacgtcga cgacgtcgat acccgtgagc acgccgagtc
     5941 cttgcacgag atccacggcc gacgacatgc gcccgcgcac gccatacgtc gcttcgaacg
     6001 acggcagcgc gggtttcacg ccttggcccc acagccagat ctgcgttgcc tgcgcgccga
     6061 catcgcgcgc ggcgcgccgc acgacgtcgc gtgacgcatc catgagtgcg cgcagcttcg
     6121 gtgccgacga gcccgtcggg aactcggcag gctgcccggt caagtcgtgc ggtggcgtgc
     6181 aatccgcgtc gctccaatcg cgcggcacga cacacaggtg ccggtactcg acaccgggga
     6241 agaacttcac gccctcgcgt ccaccgccca actcggcttc gagcgcgcgc acgatcgggt
     6301 ggctctgctc actcgagatg tgtccggccg cgaagtcgat catcgtcggc gggtcgtcgt
     6361 cggagagcgt gacgaggttg cagcggtacg cgacttcgtc gggtgcgagc tcgacaccca
     6421 tcgccgcggc ctcgatcggc gcgcgaccgg tgtggtactc gcgcgggtcg aacccgagga
     6481 tcgccatgtt gccgacgtca ctcccgggag ggagtccggg cgggatcaca tccgcgcgtc
     6541 cgacctcggc gcgttgcgcg agcgcggcga cgcgcggcat cgccgccgct tcgagcggtg
     6601 tcttgccgcc cagttcgtcg agcgcgacat ccgcgcagcc gtcgggcacg aacacgacgt
     6661 acttcatggc gcggcatcct acgggttcac cggctctagc ctccgagtcc atgcgtcgcc
     6721 tcgccgtcgt cctcttgctc gtgttcctcg cggccgcgtg cagcggcgac gacgacgaca
     6781 acgacacggc agcgtcgacg agaccgtcga cgacgaccgc ggcatcgact gcacccgttg
     6841 acagcacgtc acccaccacc gcgagcccgc ccgccgacct cgaagccgcg tcgctcacgc
     6901 tcgccgaagt cgcgtcggcg ctcgacagtc cggtcgcgct cgcgtggcgc gccggcgacg
     6961 accgcatgta cgtcgccgag cagtcgggtg cggtgcgcat cgtcgaagac ggtgcgccgc
     7021 tcgaccggcc cgtcctcgag gtcgacgtgg cgaccggcgg gaacgaacaa ggcctgctcg
     7081 gcctgacctt ctcggccgac ggcacgaagc tgtacgtcga ttacaccgac ccggacggca
     7141 acacgcacgt cgacgagtac acgatgaacg gcgacgtggc cgatacgggc tcacggcgcg
     7201 agttgttggc ggtcgaccag ccgtttgcga accacaacgg cggcgacgtg atcttcgggc
     7261 ccgacgggat gctctacatc acgctcggcg acggcggctc ggccggcgac ccgcaacagc
     7321 gcgcgcagaa cctcgacgag ctcctcggca agatcctgcg catcgacccg gcgccggcgt
     7381 ccggcgccga gtacacgatc ccgcccgaca acccgttcgt cggtcgatcc ggcgcgcgtc
     7441 ccgagatctg gatgtggggc ctgcgcaacc cgtggcggtt ctc
//
LOCUS       DASUGA010000511         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1033561, whole genome shotgun sequence.
ACCESSION   DASUGA010000511 DASUGA010000000
VERSION     DASUGA010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033561"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VFX21_10070"
     CDS             <1..380
                     /locus_tag="VFX21_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096352.1"
                     /translation="PLGAPRRYDTWFFVARAPEGQVAAHDDNEAVASAWVRPADALGA
                     YARGEIELILPTLRSVQMLARFATTGALFAALAEPMRGVITEGSGERIALLDDAPSRR
                     RWTNPLPDISWRDEQRYTEQTGS"
     gene            377..1216
                     /locus_tag="VFX21_10075"
     CDS             377..1216
                     /locus_tag="VFX21_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5096353.1"
                     /translation="MTNLTPGVPSALSPLVRRVVADNPGLMTGPGTNTYLVGIDEVAV
                     IDPGPADDKHVDAIVGASMRDRVRWVLLTHTHPDHWPAAARIRQLTGAQLAGFVKTPK
                     ADEFDLKFDLPLDEGATIDGTEFRLEVLHTPGHAPNHLCFLLEEERMLFTGDHVLNGT
                     TTVVNPQRGGDMIEYLASTERLRKLKRIARIAPGHGAVIEDPKAVLDEYVAHRKMRER
                     QIVKLLEQEPMKIPAIVEVLYVDTPEGLIEMAQRQVHAHLLKLKTEGKVRGTSVKAAW
                     SLV"
     gene            1299..>2573
                     /locus_tag="VFX21_10080"
     CDS             1299..>2573
                     /locus_tag="VFX21_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5096354.1"
                     /translation="MADTKLFINGEWVEAASGETFPSFAPSTGEKIADVAKGGRDDAQ
                     RAIEAARKAFNDGPWPKMSGKERAEKLRKVAEIITARSAEISEIEARDGGGTIKKAMF
                     ADIPGGAGSFTFFADCAENEPDEVDRGESPFPPAKSKVRREPYGVTTGIVPWNFPFIM
                     ASWKIAPSIAAGNTCVLKPASFTSLSAIEMVKACEEAGIPPGVVNLVTGPGGSAGEEL
                     AQHPEVDKVAFTGSTEVGRRIMQLASGTVKKVTLELGGKSANIVLDDADLDLAAAGVL
                     WGTFFHNGQVCESGTRALVSRKIYDEFVGTLVERAGKIVIGDNMDMATDLGPLVAPAQ
                     VDTVERYVKLGLDEGAKLLCGGSKPTDLAAGLDPNAYFLPTIFEADNSMKIAQEEIFG
                     PVLCVIPFDSDDEAIKIANDSIYGLGGGVWSKN"
BASE COUNT          446 a          875 c          874 g          378 t
ORIGIN      
        1 cgccgctcgg tgcgccgcgt cgctacgaca cgtggttctt cgtcgcgcgc gcgcccgaag
       61 gtcaggtagc ggcgcacgac gacaacgaag ccgtcgcgtc cgcgtgggtg cgtccggccg
      121 atgccctcgg cgcctacgcg cgtggcgaga tcgagctgat cctccccacg ctgcgctcgg
      181 tgcagatgct ggcccggttc gcaacgaccg gcgcgttgtt cgcggcgctg gccgagccga
      241 tgcgcggtgt gatcaccgaa ggatccggcg agcgcatcgc gctcctcgac gacgcccctt
      301 cgcgccggcg ctggaccaat ccgttgcccg acatctcctg gcgcgacgag cagcggtaca
      361 cggagcagac cgggtcgtga cgaatctcac gccgggcgtc ccgagcgcgc tgtcgccgtt
      421 ggtgcggcgc gtcgtcgccg acaacccggg gctgatgacc ggtccgggca cgaacacgta
      481 tctcgtcggc atcgacgagg tcgcagtcat cgaccccgga ccggccgacg acaagcacgt
      541 cgacgcgatc gtcggcgcgt cgatgcggga tcgcgtgcgc tgggtgctcc tcacgcacac
      601 gcaccccgat cattggcccg cggccgcgcg catccgtcag ctgaccggtg cgcagctcgc
      661 ggggttcgtg aagacgccga aggccgacga gttcgacttg aagttcgacc tgccgctcga
      721 cgaaggcgcg acgatcgacg gcacggaatt ccgtctcgag gtgctgcaca cgcccggtca
      781 tgcgccgaat catctgtgct tcctgctcga agaggaacgc atgctcttca ccggtgacca
      841 cgtgctcaac ggcacgacga ccgtggtcaa tccgcagcgg ggcggcgaca tgatcgagta
      901 cctcgcgtcg actgagcgac tgcgcaagct gaagcgcatc gcgcgcatcg cccccgggca
      961 cggcgccgtc atcgaggacc cgaaggccgt gctcgacgag tacgtcgcgc accggaagat
     1021 gcgtgagcgt cagatcgtca agctgctcga gcaagagccc atgaagatcc ccgccatcgt
     1081 cgaggtgctc tacgtcgaca cgcccgaggg gctcatcgag atggcgcaac gccaggtgca
     1141 tgcgcacctc ctcaagctca agaccgaagg caaggtacgc ggcaccagcg tcaaagccgc
     1201 ctggtcgctc gtctgagccg aacgccgccg cggggtggcc tccaccaggg gcttcgacct
     1261 agcctcgctg ggccaacaac tcgcagggag gaccgcaggt ggcggatacc aagctcttca
     1321 tcaacggcga atgggtcgag gccgcctccg gcgagacctt cccctcgttc gccccttcca
     1381 ccggcgagaa gatcgccgac gtcgccaagg gcggccgcga cgacgcccag cgggccatcg
     1441 aggccgcgcg caaggcgttc aacgacggcc cgtggccgaa gatgtccggc aaggagcgcg
     1501 ccgagaagct gcgcaaggtc gccgagatca tcaccgcgcg cagtgccgag atctccgaga
     1561 tcgaagcacg tgacggcggc ggcacgatca agaaggcgat gttcgccgac atccccggcg
     1621 gcgcgggttc gttcacgttc ttcgcggact gcgcggagaa cgagcccgac gaggtcgacc
     1681 gcggtgagag cccgttcccg cccgcgaaga gcaaggtgcg gcgcgagccc tacggcgtca
     1741 ccaccggcat cgtgccgtgg aacttcccgt tcatcatggc gtcgtggaag atcgcgccgt
     1801 cgatcgcggc gggcaacacg tgcgtgttga agcccgcgag cttcacgtcg ctgtccgcga
     1861 tcgagatggt gaaggcgtgc gaagaggccg gcatcccgcc gggtgtcgtg aacctcgtca
     1921 ccggtcccgg cggcagcgcg ggtgaagagc tcgcgcagca ccccgaagtc gacaaggtgg
     1981 cgttcaccgg ctcgaccgaa gtcggccgcc gcatcatgca gctcgcatcg ggcaccgtga
     2041 agaaggtgac gctcgagctc ggcggcaagt ccgcgaacat cgtcctcgac gacgccgacc
     2101 tcgacctcgc cgccgcgggc gtgttgtggg gcacgttctt ccacaacggt caggtgtgcg
     2161 aatcgggcac acgtgcgctc gtgtcgcgca agatctacga cgagttcgtc ggcacgctcg
     2221 tggagcgcgc gggcaagatc gtcatcggcg acaacatgga catggccact gacctcgggc
     2281 cgctggtcgc gcccgcgcag gtcgacacgg tcgagcgcta cgtgaagctc gggcttgacg
     2341 agggtgcgaa gctgctgtgc ggcggctcga agccgaccga cctcgcggcc ggcctcgacc
     2401 cgaacgcgta cttcctgccg acgatcttcg aggccgacaa ctcgatgaag atcgcccagg
     2461 aggagatctt cggcccggtg ttgtgcgtga tcccgttcga ctccgacgac gaggcgatca
     2521 agatcgcgaa cgactcgatc tacggactcg gcggtggtgt gtggtcgaag aac
//
LOCUS       DASUGA010000512         2270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_737789, whole genome shotgun sequence.
ACCESSION   DASUGA010000512 DASUGA010000000
VERSION     DASUGA010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737789"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VFX21_10085"
     CDS             complement(<1..340)
                     /locus_tag="VFX21_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096355.1"
                     /translation="MTPTRTEPTLVARSRALPDGDVLDHFVPGGMAWLGAGASFVTTG
                     VVTTVDLGDATALLGSIEHHTEGCSDQAGPRAVGALPFSGPGRLLVPARIVGRDAHGA
                     MWCTDIAPVGD"
     gene            complement(337..1023)
                     /locus_tag="VFX21_10090"
     CDS             complement(337..1023)
                     /locus_tag="VFX21_10090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HEX5096356.1"
                     /translation="MNAVLPAPAEKALTVESMFDRIAPRYDALNRALTFRMDVGWRKA
                     AVAALDLPPGSRVLDLACGTGDLCRTLADADYVPIGADFSAGMLHAARTPAPLVRGDA
                     LALPFADATFDGLTCGFALRNFVALEPVFAECARVVRPGGRIALLDVSEPSSKVVGAV
                     HGAWFRHVVPFVGGMFSDRDAYRYLPASTAYLPDASALVGMVTAAGIGHVSRREFFFG
                     TAQLVTGTRS"
     gene            complement(1020..1790)
                     /locus_tag="VFX21_10095"
     CDS             complement(1020..1790)
                     /locus_tag="VFX21_10095"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5096357.1"
                     /translation="MRTERFRVEDVARRADVSVDTIRFYQKRRLLPPPAREGRVAWYG
                     PEHIERLARIRELRAQGLTLALIGRMVSGELDATDAPLAAAVAAADGDTATDEAFLTL
                     AELAERSGVPDALLEAVARERLLVPRVQDGEARYTTADIEIVQEGLRLLEQGLPLPAL
                     LDLAREHHEATRAIAETAVGLFDEYVRAPLRESDLPDDVKAQQLVEAFRTLLPAVTAL
                     VSHHFRRVLLEVAQEHLESVGEATELAAVNAEAARNFE"
     gene            complement(1892..>2270)
                     /locus_tag="VFX21_10100"
     CDS             complement(1892..>2270)
                     /locus_tag="VFX21_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5096358.1"
                     /translation="KRGLMAAAGGLIRLPKRVPLAIALELAMTGDPIDADRALNLGLV
                     NRVVPANQVVAEAIAIAERIGENSPIAVRMSRQLVIEAAEMSEQDGWKRTLELMMPVF
                     ESGDAIEGATAFAEKRAPVWKSS"
BASE COUNT          373 a          776 c          785 g          336 t
ORIGIN      
        1 cgtcgccaac gggcgcgatg tccgtgcacc acattgcgcc gtgggcgtcg cgtccgacga
       61 tgcgggcggg cacgagcagc cggccgggac cggaaaacgg caacgcgccc accgcccggg
      121 gaccggcttg gtcggaacaa ccctcggtgt ggtgctcaat cgacccgagc aacgcagtcg
      181 cgtcgcccaa atcgaccgtg gtgaccacgc cggtcgtcac gaagctcgca cccgctccga
      241 gccaggccat gccgccgggc acgaagtggt cgagcacatc gccgtcgggc agcgcgcggc
      301 tgcgggcaac gagcgtcggt tcggtgcgcg tcggggtcac gatcgtgtcc ccgtgacaag
      361 ttgcgcggtg ccgaagaaga actctcgccg gctgacgtga ccgatgccgg ccgcggtcac
      421 catgcccacg agcgcggacg catccggcag gtacgcggtc gacgccggca gatatcggta
      481 cgcgtcgcgg tcggagaaca tgccgccgac gaacggcacg acgtgacgga accacgcacc
      541 atgcaccgcg ccgacgacct tgctcgacgg ttcggacacg tcgagcaatg cgatccggcc
      601 tccgggccgt acgacgcgcg cacactcggc gaacaccggt tccagcgcga cgaagttccg
      661 cagggcgaag ccgcacgtga ggccgtcgaa cgtcgcatcg gcgaacggca gggcgagcgc
      721 gtcaccccgc acgagcggtg ccggtgtgcg cgccgcgtgc aacatccccg ccgagaagtc
      781 ggcaccgatc ggcacgtagt ccgcgtcggc aagcgtgcgg cacaaatcgc cggtcccgca
      841 cgcgagatcg aggacacgcg agccgggcgg aaggtcgagc gcggcgaccg cggccttgcg
      901 ccagccgaca tccatgcgga acgtcagcgc ccggttcagc gcgtcgtagc gcggcgcgat
      961 gcgatcgaac atcgactcga ccgtgagggc cttttcggcc ggggcgggga gaacggcgtt
     1021 cattcgaagt tccgcgccgc ttccgcgttc acggcagcca gttcggttgc ctcgccgacg
     1081 gattcgagat gttcctgcgc gacttccaac aacacccggc ggaagtgatg cgacaccaac
     1141 gccgtcaccg ccggcagcag tgtgcggaac gcttccacga gctgctgggc tttcacgtcg
     1201 tcgggcagat ctgactcgcg caacggcgca cgcacgtact cgtcgaacag gccgacggcg
     1261 gtttccgcga tcgcgcgcgt cgcctcgtgg tgctcgcgcg cgaggtcgag caacgccggg
     1321 agcggcaggc cctgctcgag cagtcgcaga ccctcctgca cgatctcgat gtcggcggtc
     1381 gtgtagcgcg cttcaccgtc ttgcacgcgc gggacgagca accgttcgcg tgcgacggct
     1441 tcgaggagtg catcagggac gcccgaacgc tcggccagtt cggcgagcgt gaggaaggct
     1501 tcgtcggtgg cggtgtcgcc gtcggcggcc gcaacggcgg cggcgagcgg agcgtcagtt
     1561 gcgtcgagct cgcccgacac catgcgcccg atcagcgcga gggtcagccc ctgggcgcgc
     1621 agttctcgaa tgcgcgcgag ccgctcgatg tgttccggtc cgtaccatgc gacccggcct
     1681 tcgcgcgccg gcggcggcaa caggcggcgc ttttggtaga agcggatcgt gtcgacggac
     1741 acgtccgccc ggcgggcaac gtcttcgacc cggaaccgct cggtcctcac gagtactgat
     1801 tctaggagta ttcactccca agatcagaac cgcccaacat gtgtgagaaa aggccctgtc
     1861 aggggccgtt tctcacgcaa gttcgggtgg gtcaggagga cttccagacc ggggcgcgct
     1921 tctcggcgaa agcggtcgcg ccctcgatgg cgtcgccgct ctcgaacacc ggcatcatga
     1981 gttcgagcgt ccgcttccag ccgtcttgct cgctcatctc ggccgcttcg atgacgagtt
     2041 ggcgggacat ccgtacggcg atcggggagt tctcgccgat gcgctcggcg atcgcgattg
     2101 cctcggctac gacttggttt gccggcacga cgcggttcac gagtccgaga ttcagcgcgc
     2161 ggtcggcgtc gatcggatcg ccggtcatcg cgagctcgag cgcgatcgcg agcggcacac
     2221 gcttgggcag ccggatcaag ccgccggccg cagccatcaa tccgcgcttt
//
LOCUS       DASUGA010000513         2503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1254991, whole genome shotgun sequence.
ACCESSION   DASUGA010000513 DASUGA010000000
VERSION     DASUGA010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254991"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..903)
                     /locus_tag="VFX21_10105"
     CDS             complement(139..903)
                     /locus_tag="VFX21_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016906066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5096359.1"
                     /translation="MATIAITGAAGGIGRATTALLEKEGHRVIGVDLRDADVEADLST
                     PDGRLAMLAGVTELSGGTLDGVIAGAGVMDAALAVSVNYFGAVATLEGLRPLLTRSPN
                     ASAIAISSNSTTTTPGLTSAVVDACLAGDETRANALAPEGLGLAYAMSKLALARWVRR
                     NAVTADWIGAGIRLNAICPGVIETPMTKDSLEFIMGIPEVFPVPVHRPGRAEEVAGLL
                     AYLLSADAAFFCGSVIIMDGGTDAAVRADSYPTARP"
     gene            927..1697
                     /locus_tag="VFX21_10110"
     CDS             927..1697
                     /locus_tag="VFX21_10110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5096360.1"
                     /translation="MDRDKLQTLFDMTGRVAIVTGGTRGIGRAVAEGFVSAGANVVVG
                     SRKAEACTETEAHLNALGGGRAVGVATHMGELDQVQALVDRTVAAFGGVDVVVNNAAN
                     ALAQPLGEFTPEAWQKSFDVNLRGPVFLVQAALPHLRDSAHASVVNVISAGAFLFSSN
                     VAMYAAGKAGLMSFTRAMAAQFAPGIRINALAPGTVDTDMVRANTPEMQQAMAMAGHL
                     RRAADPDEMVGPVLFLASDASSFMTGQVLLVDGGLVPH"
     gene            complement(1694..2062)
                     /locus_tag="VFX21_10115"
     CDS             complement(1694..2062)
                     /locus_tag="VFX21_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004922450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX5096361.1"
                     /translation="MKANARCAARLVKFALTYPEAWEDHPWEETVVKVRKKIFVFTGM
                     HNGLLNCTVKLPESNDEALHYEFAARSGYGLGKAGWITANFEPGTDVPIDLLEEWIDE
                     SYRAVAPKTLVRQLDASRSH"
     gene            complement(2059..>2503)
                     /locus_tag="VFX21_10120"
     CDS             complement(2059..>2503)
                     /locus_tag="VFX21_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HEX5096362.1"
                     /translation="SAGHFASDVVFDDINYEHRDYDKWTAYGQSKTANILFTIELERR
                     FCAEGIHAYAVHPGMVATQLGRYMKADDMKTLSDRASSQGRTLPPMQQVDTGASTTVW
                     AATAPELDGVGGVYLVDCEISDQHAGYAFDAANARRLWEVSEALT"
BASE COUNT          372 a          837 c          875 g          419 t
ORIGIN      
        1 ggcgcgacgc gcgtcgaata cgactggttc gtgagcgaac ggattccgtt ctcgcgggcg
       61 aacacggcaa ttcgtggcct cgtcaagcgc gtcgtcggat tcgacgagga ggcgaagaac
      121 cgccacccat cggccggttc agggacgggc ggtggggtag gagtcggcgc gaaccgccgc
      181 gtcggtgccg ccgtccatga tgatcacgga cccgcagaag aacgcagcgt cggctgacag
      241 caagtacgcg agcagccccg cgacttcttc ggcgcggccc ggccggtgca ccggcaccgg
      301 gaacacctcc ggaatgccca tgatgaactc gagggagtcc ttcgtcatcg gcgtctcgat
      361 cacgccgggg cagatcgcgt tcagccggat gccggcgccg atccagtcgg ccgtcaccgc
      421 gttgcgccgg acccagcgcg ccaacgcgag cttcgacatc gcgtaggcga gcccgagccc
      481 ttcgggtgcg agcgcgttcg ctcgcgtctc gtcgccggcc aggcacgcgt cgacgacggc
      541 gctcgtgagc ccgggcgtgg tcgtcgtcga gttggagctg atcgcgatcg cgctggcgtt
      601 gggtgaacgc gtcagcagtg ggcgcagccc ttcgagcgtc gcgaccgcgc cgaagtagtt
      661 caccgacacc gcgagcgcag cgtccatcac gcccgcgccg gcgatcacac cgtcgagcgt
      721 gccgccgctg agctcggtga cgcccgccaa catcgcgagg cgcccgtctg gtgtcgacag
      781 gtcggcttcg acgtcggcgt cgcgcaggtc gacgccgatg acccggtggc cttctttctc
      841 gagcagcgca gtggtggcgc ggccgatgcc gcccgccgcg ccggtgatcg cgatcgtcgc
      901 catctccgta cgctagacct gcgtccatgg accgggacaa gctgcagaca ctgttcgaca
      961 tgacgggtcg cgtcgcgatc gtcacgggtg gcacgagggg catcgggcgc gcggttgccg
     1021 aggggttcgt gagtgcgggc gcgaacgtcg tggtcgggag ccgcaaggcc gaggcgtgca
     1081 cggagaccga agcgcacctg aacgcgctcg gcggcgggcg ggccgtcggt gtggcgacgc
     1141 acatggggga gctcgaccag gtgcaggcgc tcgtcgaccg cacggtcgcc gcgttcggcg
     1201 gcgtcgacgt cgtggtcaac aacgcggcca acgcgctcgc ccagccgctc ggcgagttca
     1261 cgcccgaggc gtggcagaag tcgttcgacg tcaacctgcg cggaccggtg ttcctcgtgc
     1321 aggcggcact gccgcacttg cgtgacagcg cccatgcgtc ggtcgtgaac gtgatctcgg
     1381 ccggcgcgtt cttgttctcc tccaacgtcg cgatgtacgc ggcgggcaag gcggggttga
     1441 tgtcgttcac gcgcgcgatg gccgcgcagt tcgcacccgg catccgcatc aacgcgcttg
     1501 cgcccggcac tgtcgacacc gatatggtgc gggccaatac gcccgagatg cagcaggcga
     1561 tggcgatggc cggtcacctg cgccgcgcgg ccgatcccga cgagatggtc ggcccggtgc
     1621 tgttcctcgc gtccgacgcg tcgagcttca tgaccggtca ggtgttgctc gtcgacggcg
     1681 gcctggtgcc gcactagtga gagcgtgacg cgtcgagttg gcgcacgagc gtcttcggcg
     1741 cgaccgcgcg gtagctctcg tcgatccact cctcgaggag gtcgatggga acgtcggtgc
     1801 ccggttcgaa gttcgcggtg atccagcccg ccttgccgag cccgtagccc gaacgggccg
     1861 cgaactcgta atgcaacgct tcgtcgttgc tctccggcag tttgaccgtg cagttcaaga
     1921 gaccgttgtg catgccggtg aacacgaaga tcttcttgcg gaccttgacg acggtctcct
     1981 cccagggatg gtcctcccac gcctcggggt acgtgagcgc gaacttcacc aaccgtgccg
     2041 cgcaacgcgc attcgccttc atgtcagcgc ttccgagact tcccacagcc gccgggcgtt
     2101 ggccgcgtcg aacgcgtagc cggcgtgttg gtcgctgatc tcgcagtcga ccaggtagac
     2161 accccctacc ccgtcgagct cgggcgccgt cgcggcccaa acggtcgtcg acgcaccggt
     2221 gtcgacttgt tgcatcggcg gcaacgtgcg gccctggctg ctcgcccggt cggagagcgt
     2281 cttcatgtcg tcggccttca tgtagcggcc gagctgcgta gccaccatgc ccggatgcac
     2341 ggcgtacgcg tggatgccct cggcacagaa gcgtcgttcg agctcgatcg tgaacaagat
     2401 gttcgcggtc ttcgactgcc cgtacgcggt ccacttgtcg tagtcgcggt gctcgtagtt
     2461 gatgtcgtcg aacacgacgt cggatgcgaa gtggccggcc gag
//
LOCUS       DASUGA010000514         5887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1007669, whole genome shotgun sequence.
ACCESSION   DASUGA010000514 DASUGA010000000
VERSION     DASUGA010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007669"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /locus_tag="VFX21_10125"
     CDS             complement(<1..1272)
                     /locus_tag="VFX21_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096363.1"
                     /translation="MTVRWVAVFGVVAFVLAACYPPVPPGDSYAVRWGTAMSGPTNED
                     EFDGIAAGAGGSAFVTGKFEQSVTLGGTTLTSAGRADIPLARFDQFGRPLWVQRFGGT
                     GEDNLFDIDADANGAVGTGWFAGTVDFGPVRLRAAGASDCVVVAVDNAGAVRWARALG
                     GPYRDGCNEVTIAADGSIVTSLDTEGGWTPPGHAAIPRLDFADTLLVHLSPGGDVQWM
                     RRVGGPGPQRGKSLAVAPDGSIVFGGDTIGGLDVAGTAVTVPNMNRDAWISRWSAAGA
                     QQWTKTWGGPGDDLAKGVVATADAVFVVGPFTGATSFGGVTLDAGTNADLAVVRFSTS
                     GTLAWATSVRAPGPVDGAEAIAAADGGLLFGSTVTAGLQFGSAGGAYVPVDTSKGGTA
                     WLAHYRPDGSLGFAATIAGTANGNIGEIARGD"
     gene            complement(1307..2380)
                     /locus_tag="VFX21_10130"
     CDS             complement(1307..2380)
                     /locus_tag="VFX21_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5096364.1"
                     /translation="MALPVSDADRVGRIARAGLDHVALQLEADALMRADVGYDVVAWG
                     TVDPAALLITGCTPIGAHPHSPELHLRNFDIEHLCGEPLTIRTLLGRDQPVASLRDEV
                     LDIERTRRYQEFLAPYDFVDELVAVLRVDGRCWGAVRAYRHDEPFTARDRASFASVSG
                     TLAAGLRRVFLHAATEHPLALDDPPGMCTLDREGRVVACTRPAHDWLDSLMGEGSVTA
                     ILQSLAGRVAEHGDASAVAVGRRGPIALHATCIGADGEMAVVIERPRPIQLTPRIMEA
                     YGLTAREMHVTELVLRGLTTAQIAHEAGISRYTVQDHLKSIFAKVGVQTRGELAHEIY
                     VRFYLPPKQAGMTPGPYGYFLGL"
     gene            complement(2630..3457)
                     /locus_tag="VFX21_10135"
     CDS             complement(2630..3457)
                     /locus_tag="VFX21_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006544012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5096365.1"
                     /translation="MKADAFRYDGKRAVVVGGATGMGAATAELVQDLGAEVVVMDFAP
                     VTLQGVKVISLDLRDKDSIDNAVDECGGPVDALFSCAGVADGTPGIEKINFIGHRRFI
                     NRAIDKGFLGRGGAIGMISSAAGLGWEANLALVNEYLAIEDFDAASEWAVSNLKADYM
                     FSKQTICAYVAGQAFPMQKKGIRINAILPGPTDTPLAQANADMWLGFGADYREEAGIE
                     PSRSDEQAGPLAFLCSDAASYVNGVTLITDAGYISSGLTESFPPAGPAAQFLYGRLT"
     gene            3649..4518
                     /locus_tag="VFX21_10140"
     CDS             3649..4518
                     /locus_tag="VFX21_10140"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEX5096366.1"
                     /translation="MSEAFMIMLREGFEATLIVAIVFTYLRKIDRLDLGAAVWTGVAF
                     AAGIATGLGLILHWTIGDFEGRARMLTFAAICATAAVVLTWMIFWMRKQSRAIKNELH
                     EKVDAAIASDAARRGVMFVAFIAVLREGIESALFLVAAATGDDSNGVLIGAIAGLIAA
                     CVLGYLVYSGGRQIPMATFFKVTGILLILFAAGLLSRTVFFLQAAGDIGTMNNAFYNV
                     TGIHWLTVDTQTGRFLAGIFGWDPRPSIEQVIVYLGYVIPVTYLFLRASKGKAPAPAP
                     THAAPQPVPSAGG"
     gene            4556..5311
                     /locus_tag="VFX21_10145"
     CDS             4556..5311
                     /locus_tag="VFX21_10145"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX5096367.1"
                     /translation="MRLALISDIHGNSIALDAVLADVAAMGGADAWWVLGDLVALGHD
                     PVGVLERVAALPNAQYLSGNTERYVLTGAVPFPRAEHVAADPSLAPRALEVTANFAWT
                     RGIVTYAGWWDWLCALPRTVRTELPDGTRVLGVHASPGSDDGPGISTRIDDDALAQLL
                     DGCDADIVFGGHTHDAVDRVVGDLRAVNLGSVGNSKRADRRASYVVVDADEHGHTIEH
                     RLVDYDHEAVCAAIDAVAHPAGHYLKTFQVVTS"
     gene            complement(5308..>5887)
                     /locus_tag="VFX21_10150"
     CDS             complement(5308..>5887)
                     /locus_tag="VFX21_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018569808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5096368.1"
                     /translation="ALLDQRSDEFAVSVLAPLSTAQRDRLVAAMADVERLLTASMVDV
                     APIDPSHPHAAFCLRSYYAELDRRFTTGFDPDSSLAVLEDEIRPPAGVMLVATLHSEP
                     VGCGCLKFHGRAPTEIKRLWVADAVRGLGIGRRLLRELEQRAAQSESRVVRLDTNAAL
                     VEAIAMYRSNGYSEIPAFNDEPYANNWFEKQL"
BASE COUNT          887 a         2084 c         1975 g          941 t
ORIGIN      
        1 gtcgccgcgg gcgatctcgc cgatgttgcc gttcgccgtc cccgcgatcg tcgccgcgaa
       61 cccgagggat ccgtcggggc ggtagtgcgc gagccacgcg gtgccgccct tgctcgtgtc
      121 caccggcacg taggcgcctc cggccgatcc gaactgcagg ccggccgtga cggtgctccc
      181 gaacaacaag ccgccgtcgg cggccgcgat cgcttcggcg ccgtcgacgg gtcccggggc
      241 gcggaccgac gttgcccacg cgagcgtgcc actcgtcgag aagcgcacga ccgcgagatc
      301 cgcattcgtg cccgcatcga gcgtcacgcc gccgaaggaa gtcgcgccgg tgaacggacc
      361 gacgacgaat accgcgtcgg ccgtcgcgac cacgcccttc gcgaggtcgt cgccggggcc
      421 tccccaagtc ttcgtccact gctgcgcgcc ggccgcactc catcgcgaga tccacgcgtc
      481 gcgattcatg ttcgggaccg tcacggccgt gcccgcgacg tcgaggccgc cgatcgtgtc
      541 gccgccgaac acgatcgatc cgtcgggcgc gaccgcgagc gacttgccgc gctgcggtcc
      601 gggaccgccg acgcgtcgca tccactggac gtcgccaccg ggcgacagat gaacgagcag
      661 cgtgtcggcg aagtcgagcc gggggatcgc ggcgtgcccg ggcggtgtcc atccgccttc
      721 ggtgtcgagt gacgtgacga tcgaaccgtc cgccgcgatc gtgacttcgt tgcagccgtc
      781 gcggtacggg ccgccgagcg cgcgcgccca ccgtactgct cccgcgttgt cgaccgcgac
      841 gacgacacag tcggacgcac ccgcggcgcg caaccggacc gggccgaagt cgacggtccc
      901 ggcaaaccat cccgtcccca ccgcgccgtt cgcgtccgcg tcgatgtcga acaagttgtc
      961 ctcgccggtc ccaccgaaac gctgcaccca cagcggccgg ccgaactggt cgaagcgggc
     1021 caggggaatg tcggcgcgac cggcgctcgt cagcgtcgtc ccgccgagtg tcaccgattg
     1081 ttcgaacttg ccggtgacga acgcactgcc gcccgcgccg gcggcaatgc cgtcgaactc
     1141 gtcctcgttc gtgggccccg acatggccgt gccccaccgg accgcgtacg agtcgcccgg
     1201 cgggaccggc ggatagcacg ccgcgagcac gaacgccacg acgccgaaga cggcgaccca
     1261 tcgcacggtc atggcccgac gctaacgagc gattgtcagg ggattgttag agaccgagga
     1321 agtagccata cggacccggt gtcatgcccg cctgcttggg gggcaggtag aagcgcacgt
     1381 agatctcgtg cgcgagctcg ccgcgcgtct gcacgccgac cttggcgaag atcgacttga
     1441 ggtggtcttg caccgtgtag cgcgagatgc cggcctcgtg ggcaatctgc gcggtcgtga
     1501 gtccccgcag cacgagctcg gtcacgtgca tctcgcgcgc cgtcaggccg tacgcctcca
     1561 tgatgcgcgg cgtcagctgg atcggacgcg gccgttcgat caccaccgcc atctcaccgt
     1621 ccgcgccgat gcaggtcgcg tggagtgcga tcgggccgcg tctgccgacg gcgactgccg
     1681 acgcgtcgcc gtgctcggcg acccgaccgg ccagcgattg caagatggcg gtgaccgacc
     1741 cctcgcccat caggctgtcg agccagtcgt gggccggccg ggtgcaggcc acgactctgc
     1801 cttcgcggtc gagcgtgcac atgccgggtg gatcgtcgag cgcgagcgga tgctcggtgg
     1861 ccgcgtggag gaacacgcgc cgtaagcccg cggccaatgt cccgctcacg gacgcgaagg
     1921 acgcgcgatc acgcgccgtg aacggctcgt cgtgccggta cgcgcgaacc gcgccccaac
     1981 accgcccatc gacccgcaag accgcgacga gctcgtcgac gaagtcatac ggcgcgagga
     2041 attcctgata gcgccgcgtg cgctcgatgt cgagcacttc gtcccgcagt gatgccaccg
     2101 gttggtcgcg tccgagcagc gtgcggatgg tcagcggttc gccgcagaga tgttcgatgt
     2161 cgaagttgcg caggtgcaac tccggcgaat gcgggtgcgc accgatgggt gtacaacccg
     2221 tgatcaacag cgccgcggga tcgacggtcc cccaggcgac gacgtcgtag ccgacgtcgg
     2281 cgcgcatgag cgcatcggct tcgagttgca gtgcgacgtg atcgagcccc gcccgggcga
     2341 tcctgcccac ccggtcagcg tcgctgacgg ggagtgccat gcacatcacc ttatggaccc
     2401 cacacatctg ggatttctgt ttcgcggcac caactcgtgc aactcgatct cgaacccggc
     2461 gactacgcgg tcttctgttc gatcccgtca ccggacgaga cgccgcacca cgcgaaaggc
     2521 atgctcacga gggtgacggt cacatgagtg cgaacgccgt cctcgagacc cgactcgtcc
     2581 acggcgtgca ccgccgcgcc agtggactgc ggctttctcg ccgagatggc tacgtgaggc
     2641 ggccgtacag gaactgcgcg gccggccccg cgggcgggaa cgactccgtc agccccgagc
     2701 tgatgtagcc cgcgtcggtg atgagcgtca cgccgttgac gtacgaggcc gcgtcgctgc
     2761 acaggaaggc cagcggtccc gcttgctcgt cggaccgcga cggttcgatc cccgcttcct
     2821 cgcggtagtc ggcgccgaag ccgagccaca tgtcggcgtt ggcctgcgcg agcggcgtgt
     2881 ccgtcggccc gggaaggatc gcgttgatgc ggatgccctt cttctgcatc ggaaacgcct
     2941 ggcccgccac gtacgcgcag atggtctgct tgctgaacat gtagtcggcc ttcaagttcg
     3001 agaccgccca ctcggacgcc gcatcgaagt cctcgatcgc gaggtactcg ttcaccagcg
     3061 cgaggttcgc ttcccatccc agacccgcag ccgacgagat catgccgatc gcgccgccgc
     3121 gaccgaggaa gcccttgtcg atcgcgcggt tgatgaaccg gcggtgaccg atgaagttga
     3181 tcttctcgat cccgggcgtg ccgtcggcga ctccggcaca cgagaacagc gcgtcgaccg
     3241 gcccaccgca ctcgtcgacg gcattgtcga tgctgtcctt gtcccgcagg tcgaggctga
     3301 tcaccttgac gccctgcaac gtcaccggtg cgaagtccat gacgacgact tcggcaccga
     3361 ggtcctggac gagctcggcc gtcgcggcgc ccatcccggt ggcgccgccg accacgactg
     3421 cccgcttgcc gtcgtaccgg aacgcatcag ccttcatgtg gggtctcctc ggtcgtcggg
     3481 gtgtccatga ttgcccgcgc cgaggcccgc gggcatctcc accgccgccc cgaccggcgc
     3541 cggcgagccc accccggcgc ggccgcccca cccgctctcg accccggcgc tttccgggac
     3601 cggggtttgt gtggtatgcc ttacctaagt attcggtcag gaggccaggt gagcgaagcg
     3661 ttcatgatca tgctgcggga gggcttcgag gccacgttga tcgtggcgat tgtgttcacc
     3721 tacctgcgca agatcgaccg cctcgacctc ggtgccgcgg tctggaccgg cgtcgcgttc
     3781 gcggccggca tcgcgaccgg gctcggcctg atcctgcact ggacgatcgg cgacttcgag
     3841 ggccgggccc gcatgctcac gttcgcggcg atctgcgcga ccgcggcggt ggtgctgacg
     3901 tggatgatct tctggatgcg caagcagtcg cgcgcgatca agaacgagct gcacgagaag
     3961 gtcgacgccg ccatcgcctc cgacgccgcg cgccgcggtg tcatgttcgt cgccttcatc
     4021 gcggtgttgc gtgagggcat cgagtcggcg ctgttcctcg tcgccgccgc gaccggtgac
     4081 gactcgaacg gtgtgctcat cggcgcgatc gccgggctca tcgccgcgtg cgtgctcggc
     4141 tacctcgtgt actcgggcgg ccgccagatc ccgatggcga ccttcttcaa ggtgacgggc
     4201 atcttgctca tcctcttcgc cgcgggcttg ctctctcgca ccgtcttctt cctgcaggcc
     4261 gcgggcgaca tcggcacgat gaacaacgcg ttctacaacg tcaccggtat ccactggttg
     4321 accgtcgaca cgcagaccgg ccgcttcctg gcgggcatct tcggttggga cccgcgcccc
     4381 tccatcgagc aagtgatcgt ctacctcggg tatgtgatcc cggtgacgta cctgttcctg
     4441 cgggcgagca agggcaaggc gccggcaccc gcgccgacgc acgccgcacc gcagcccgtc
     4501 ccctccgccg gcgggtagca cagccggacg agcgggacgc gcgacagact gggccgtgcg
     4561 gctcgcgctc atctccgaca tccacggcaa ttccatcgcc ctcgacgccg tgctcgccga
     4621 cgttgccgcg atgggcggcg ccgacgcgtg gtgggtgctc ggtgacctcg ttgcgctcgg
     4681 acacgatccg gtcggtgtgc tcgaacgggt cgccgccctg ccgaacgcgc agtacctgtc
     4741 gggcaacacc gagcggtacg tgctcaccgg tgccgttccg ttcccccgag cggaacacgt
     4801 tgccgcggat ccgtctctcg cgccgcgcgc gctcgaggtc acggcgaact tcgcgtggac
     4861 gcgcggcatc gtcacctacg ccggttggtg ggattggttg tgcgcactcc cgcgaaccgt
     4921 gcggaccgag ctccccgacg gcacgcgcgt gctcggcgtc cacgccagcc ccggttcgga
     4981 tgacggaccc ggcatcagca cgcgcatcga cgacgacgcg ctcgcgcagt tgctcgacgg
     5041 ctgtgatgcc gacatcgtgt tcggcggcca cacgcacgac gcggtcgatc gggtcgtcgg
     5101 cgacctacgg gccgtgaatc tcggaagcgt cgggaactcc aagcgcgccg accggcgcgc
     5161 ctcgtacgtc gtggtcgacg cggacgaaca cggtcatacg atcgagcacc gcctcgtcga
     5221 ctacgaccac gaagctgtct gtgctgcgat cgacgcggtt gcgcatcccg cggggcacta
     5281 cttgaagacc tttcaggtgg tgacgagcta gagctgcttc tcgaaccagt tgttcgcgta
     5341 cggctcgtcg ttgaacgccg ggatctccga gtagccgttc gaccggtaca tcgcgatcgc
     5401 ttcgacgagc gccgcgttgg tgtcgagccg cacgacacga ctctcgctct gtgccgcccg
     5461 ttgttcgagc tcgcgcaaca accggcggcc gatgccgagc ccgcgcacgg cgtcggcgac
     5521 ccagagccgt ttgatctcgg tcggtgcccg gccgtggaac ttcaggcagc cgcaaccgac
     5581 cggctcgctg tgcaacgtgg cgacgagcat cacgcccgcg ggcggtcgga tctcgtcttc
     5641 gagcaccgcg aggctcgagt cggggtcgaa ccccgtcgtg aagcggcggt cgagctccgc
     5701 gtagtacgag cgaaggcaga acgctgcgtg gggatgcgac ggatcgatcg gcgcgacgtc
     5761 gaccatcgac gcggtgagca accgttcgac gtcggccatc gcggcaacga gtcggtcgcg
     5821 ttgcgccgtg ctcagcggtg cgagcaccga cacggcgaac tcgtcgctgc gttggtcgag
     5881 gagcgcg
//
LOCUS       DASUGA010000515         4773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1267868, whole genome shotgun sequence.
ACCESSION   DASUGA010000515 DASUGA010000000
VERSION     DASUGA010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267868"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1475
                     /gene="fliD"
                     /locus_tag="VFX21_10155"
     CDS             <1..1475
                     /gene="fliD"
                     /locus_tag="VFX21_10155"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HEX5096369.1"
                     /translation="REGTLTGKNVATGGGSLATLVANINAAGAGVTATAVQVGTNLYR
                     LQITSNTAGALNGTNIASTELDATVGGLVTLTAASDAQVTVGEGDGAYTVTSATNTLS
                     GLLPGVTVTLKHETDELVTIDASRDVSSLSKNVQAFVDAMNELKDNITTLTKFDPETK
                     QASVLTGDSASRRLLSDLSRAVTDAVPWARPSSPGLAGLSIDKEGKYVFDESKFTTAF
                     NADPEGVTALFTQGGTATSSDVTFVSAGDRARGGTYAVEITQVATQATNTGLSGSWPI
                     GTPPTIKVRVGSTEITYDIGATDDQDDVVRALNTKFGLEELQLSASVDGTGIKITTTG
                     YGSSARFDVDWGDGNYTNYAGLDVQGTIGGVTATGNGQQLSIPFDNSEMGGLALSITA
                     TAIGSLGTFGYQPGVGARTVTSLLDATDVLTGYITSTEKALKARVSFIDTQVESMERR
                     LEQYQTRLKAYYAQLETTLSTLQQQSQWLAGQVAGLNAQQ"
     gene            1505..1906
                     /gene="fliS"
                     /locus_tag="VFX21_10160"
     CDS             1505..1906
                     /gene="fliS"
                     /locus_tag="VFX21_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015625757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HEX5096370.1"
                     /translation="MTSNAALRDRYRADSVQTMSPGRLIVALYNRLLLDLDRAMAALG
                     IENVVAAHAALIHAQEIVGELHDSLDVDAWPPARQLADIYVYVTSELVAANLAKDAAR
                     VYACKTIIEPLRDAWQEAAGLVPDDPNAIVA"
     gene            1903..2211
                     /locus_tag="VFX21_10165"
     CDS             1903..2211
                     /locus_tag="VFX21_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096371.1"
                     /translation="MSEQLVREWTAALDDFETTLAVCEATLDPSADAPVPTPFSAPAC
                     SGPIPRDLTPRAEDLMRRSRDLQRRLVLEQQRIRAEIQRLPRVPGTASASGETVDFGA
                     "
     gene            2242..3981
                     /locus_tag="VFX21_10170"
     CDS             2242..3981
                     /locus_tag="VFX21_10170"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5096372.1"
                     /translation="MRTQATGAVDDLLERGLDLVARRNFEGAFVAYRQAIDLAPDDPR
                     GHMLLGAALSEHGTADEAVAELELAADLAGPLCDETWALYNNLANALRRAERYDGAEQ
                     ILLELTKFAPKEWQPWHNLGQLYRDTKRNDEAAAALRRAIALAPDFAPNHALLGAVLH
                     QLDRLHSADAAFNRAFALGYDDDPNMWTLYGNNCRFLARRDDCIAALERALEMQPGSP
                     PAYSNLASIKTTCGRFAEARTLIDRAVELEPENSLWRANRGFMLLTSGDLDQGWTDWE
                     FAVQNGTRGNERPLDVPRWTPEHPDARVMVYREQGVGDEILFASCYGDLIDCARDVVI
                     ECTDRLLPLFARSFPGAEVREQSWKAGLGETKNDVDMALPSGSLPQHFRPTVSSFSGE
                     RRSFLVPDAAKVEKWRARFAAFGQGAVVGMSWRSKLTTAERRLEYTRLADDWGPLFAV
                     EGVHWVNLQYDDCERQLRDAEAQYGVTVHRWADVDYMNDFDEVAAIMAACDLVVAPRN
                     AVAMLAGALGVPTVMMGNRWDWADLGTDTSPWFPSVEIAYRHFGEEWNEVIATAARRV
                     AAVAAGAGSATRD"
     gene            3974..>4773
                     /gene="pseB"
                     /locus_tag="VFX21_10175"
     CDS             3974..>4773
                     /gene="pseB"
                     /locus_tag="VFX21_10175"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017470751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HEX5096373.1"
                     /translation="MIDLNGKTILLTGGTGSFGNAFLTKVTTTWPDTVVRVYSRDELK
                     QSELRAKFGDEQVRYLIGDVRDRARMSRAAEGADVIVHAAAMKQVEACEYNPFEAVRT
                     NVLGAQHVVDSAIDARVPRVVALSTDKAVNPVNLYGATKLCQEKIFVQGNAYAAQRET
                     RLSCVRYGNVVGSRGSVVPVFRQQLKRDGRITITDDRMTRFWIMLPQAVDLVLYALEH
                     MVGGEVFIPKIPSMRVTDLADAVAPNVPRETIGIRPGEKLHELMITAD"
BASE COUNT          840 a         1646 c         1609 g          678 t
ORIGIN      
        1 tgcgcgaagg cacgctgacc ggcaagaacg tcgcaaccgg cggcggcagt ctggccacgc
       61 tcgtcgcgaa catcaacgcg gccggcgcgg gcgtgacggc gaccgcggtg caggtcggca
      121 cgaacctgta ccgcctgcag atcacgtcga atacggcggg tgcgctgaac gggacgaaca
      181 tcgcgagcac cgagctcgac gcgaccgtgg gcggcctggt cacgctgact gcggcgtccg
      241 acgcgcaggt cacggtcggc gagggcgacg gcgcctacac ggtgacgtcg gcaacgaaca
      301 cgctgagcgg actgctgccc ggcgtcaccg tcacgttgaa gcacgagacc gacgagctcg
      361 tcacgatcga cgcgtcacgc gacgtgagct cgctcagcaa gaacgtgcag gcgttcgtcg
      421 acgcgatgaa cgagttgaag gacaacatca ccacgctgac gaagttcgac ccggagacga
      481 aacaggcgtc ggtgctcacg ggcgactccg cgagccgccg cctgctctcg gatctgagcc
      541 gggccgtcac cgacgcggtg ccgtgggcgc ggccgtcgag cccggggctc gcggggctgt
      601 cgatcgacaa ggaagggaaa tacgtcttcg acgagtcgaa gttcacgacc gcattcaacg
      661 ccgaccccga aggggtgacc gcgctgttca cgcagggcgg caccgcgacg tcgagcgacg
      721 tgacgttcgt gtcggcgggc gaccgggcgc gaggcggcac gtatgcagtc gagatcacac
      781 aagtcgcgac gcaagcgacc aacaccggac tctcggggtc gtggccgatc ggcacacccc
      841 cgacgatcaa ggtccgcgtc ggcagcaccg agatcaccta cgacatcggc gcgaccgacg
      901 accaagacga cgtcgtgcgc gcgctgaaca ccaagttcgg gctcgaggag ctgcagctgt
      961 cggccagcgt cgacggcacg ggcatcaaga tcacgacgac cggctacgga tcgtccgcgc
     1021 gcttcgacgt cgactggggc gacggtaact acaccaacta cgccggtctc gacgtccagg
     1081 gaacgatcgg cggcgtcacc gcgaccggca acgggcaaca gctcagcatt ccgttcgaca
     1141 actccgagat gggtgggctc gcgctctcca tcaccgcgac tgcgatcggc agcctgggca
     1201 cgttcggcta ccagcccggc gtcggcgcgc gcaccgtgac cagcctgctg gatgcaacgg
     1261 acgtgctcac cggctacatc acgtcgacgg aaaaagcctt gaaagcgcgg gtttcgttca
     1321 tcgatacgca agtcgagtcg atggaacgtc gtctggagca ataccagact cggctgaagg
     1381 cgtactacgc gcagttggaa acgacgctga gcacgctgca gcagcaatcg cagtggctgg
     1441 ccggacaagt cgccggcctg aacgcacaac aatgacgaat cgctacgtcg ggaacggagt
     1501 ccccatgacg agcaacgcgg cactccgcga tcgctatcgc gctgactcgg tgcagacgat
     1561 gtcgcccgga cgcctcatcg ttgcgctgta taaccgcttg ctcctcgatc tcgaccgcgc
     1621 gatggccgcg ctgggtatcg agaacgtcgt cgccgcccac gccgcgctca tccacgcgca
     1681 ggagatcgtc ggtgaactgc acgactcgct cgacgtcgac gcgtggccgc cggcgcgcca
     1741 gctggcggac atctacgtct acgtgacctc cgagctcgtg gccgcgaacc tggccaagga
     1801 cgcggcccgc gtctacgcgt gcaaaacgat catcgagccg ctccgggacg cgtggcagga
     1861 agcggccggc ctggtgcccg acgacccgaa cgcgatcgtc gcgtgagcga gcagctcgtg
     1921 cgggagtgga ccgcggcgct cgacgacttc gagacgacgc tggcggtctg cgaagcgacg
     1981 ctcgatccgt ctgccgacgc tcccgtcccc acaccgttct cggcgcccgc gtgctcggga
     2041 ccgatcccgc gcgatctcac cccgcgcgcc gaggacctga tgcgccggtc ccgtgacctc
     2101 caacgccgct tggtgctcga acagcagcgg atccgggccg agatccagcg cctgccgcgg
     2161 gtacccggaa ccgcctcggc cagcggggaa acggtcgact tcggcgcctg aagaccgggg
     2221 cccgccggcc gataagagtg cgtgcggacc caggcgaccg gagcggtcga cgatctcctc
     2281 gaacgcggcc tcgacctggt cgcccgccgc aacttcgagg gcgcgttcgt ggcgtaccgc
     2341 caagccatcg acctcgcgcc cgacgacccg cgcggccaca tgctgctcgg cgccgccctc
     2401 tcggagcacg gcaccgccga cgaagcggtc gccgagctcg agctggccgc cgacctcgcc
     2461 gggcccctgt gcgacgagac gtgggcgctc tacaacaatc tcgcgaacgc gctacgacgc
     2521 gccgagcgct acgacggcgc cgagcagatc ctcctcgagc tgacgaagtt cgcaccgaag
     2581 gagtggcagc cctggcacaa cctcggccag ctctaccgcg acacgaagcg caacgacgag
     2641 gcggccgcgg cgctgcgccg cgcgatcgcg ctcgcacctg acttcgcacc gaaccacgcg
     2701 ctcctggggg cggtgctgca tcaactggac cggttgcaca gcgccgacgc cgcgttcaac
     2761 cgcgcgttcg cgctgggtta cgacgacgac ccgaacatgt ggacgttgta cggcaacaac
     2821 tgtcgttttc tcgcgcgaag ggacgactgc atcgcggcgc tggagcgcgc gttggagatg
     2881 cagccagggt cgccgcccgc gtacagcaac ctggcgtcga tcaagaccac ctgcggccgc
     2941 ttcgccgaag cgcgcacctt gatcgaccgc gcggtcgaac tcgagcccga gaacagcctg
     3001 tggcgcgcca accgcggctt catgctgctc acgtccggtg acctcgacca gggctggacc
     3061 gactgggagt tcgcggtgca gaacggcacc cgcggcaacg agcggccgct cgacgtgccg
     3121 cgctggacgc ccgagcaccc cgacgcgcgc gtgatggtgt atcgcgaaca aggcgtcggg
     3181 gacgagatcc tcttcgcgtc gtgctacggc gacctcatcg actgcgcgcg cgacgtcgtc
     3241 atcgagtgca ctgaccggct cctgccgctg ttcgcgcgct cgttccccgg cgcggaggtc
     3301 cgcgagcagt cgtggaaggc cggtctcggc gagacgaaga acgacgtcga catggcgctg
     3361 ccctcgggca gcctgccgca acacttccgg ccgaccgtct cgtcgttctc gggggagcgc
     3421 cggtcgttcc tggtgcccga cgcggcgaag gtcgagaagt ggcgcgcgcg atttgcagcc
     3481 ttcggtcagg gtgctgtcgt cggcatgtcg tggcgcagca agctcacgac agctgaacgg
     3541 cgactcgaat acacgcgcct tgccgacgac tgggggccgc tgttcgccgt cgagggcgtg
     3601 cactgggtca acctccagta cgacgactgc gaacgccaac tgcgcgacgc cgaagcgcag
     3661 tacggcgtga cggtccaccg ctgggcagac gtcgactaca tgaacgactt cgacgaggtc
     3721 gccgcgatca tggccgcgtg tgacctcgtt gtcgcgccgc gcaatgcggt cgcgatgctg
     3781 gccggcgcgc tcggcgtgcc gaccgtgatg atgggcaatc ggtgggactg ggcggatctc
     3841 ggcaccgaca cgtcgccgtg gttcccgtcc gtcgagatcg cctaccgaca cttcggtgag
     3901 gaatggaacg aggtcatcgc gactgcggcg cgccgcgtcg ccgcggtggc cgccggcgca
     3961 gggagcgcaa cgcgtgattg atctcaatgg caagacgatc ctgctgaccg ggggcacggg
     4021 ttccttcggg aacgcgttcc tcaccaaggt caccacaacg tggccggaca ccgtcgtgcg
     4081 cgtgtactcg cgtgacgagc tcaagcagtc ggagctgcgc gccaagttcg gcgacgaaca
     4141 agtccgctac ctgatcggcg acgtccgcga ccgcgcgcgt atgtcgcggg ctgccgaagg
     4201 cgccgacgtc atcgtccatg ccgccgcgat gaagcaggtc gaggcgtgcg agtacaaccc
     4261 gttcgaagcc gtccgcacga acgtgttggg cgcgcagcat gtcgtcgact cggccatcga
     4321 tgctcgcgtg ccgagggtcg tcgcgctgtc gaccgacaag gccgtcaacc ccgtgaacct
     4381 ctacggcgcg acgaagctgt gccaggagaa gatcttcgtg cagggcaacg cgtacgcggc
     4441 gcaacgcgag acgcgactct cgtgcgtgcg gtacggcaac gtcgtcggtt cccgcggctc
     4501 ggtcgtgccg gtgttccgcc aacaactgaa gcgcgacggc cgcatcacga tcaccgacga
     4561 ccgcatgacc cgcttttgga tcatgctccc ccaggcggtc gacctcgtgc tgtacgcgct
     4621 cgagcacatg gtcggcggcg aggtgttcat cccgaagatc ccgtcgatgc gggtgaccga
     4681 cctcgccgac gcggtcgcgc cgaacgtgcc gcgcgagacg atcgggatcc gtcccggcga
     4741 gaagctccac gagctcatga tcaccgcgga cga
//
LOCUS       DASUGA010000516         2028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1693779, whole genome shotgun sequence.
ACCESSION   DASUGA010000516 DASUGA010000000
VERSION     DASUGA010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693779"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VFX21_10180"
     CDS             complement(<1..282)
                     /locus_tag="VFX21_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096374.1"
                     /translation="MFAVPYAEIRELQRDALRYRFDTLRPRVQLLDRLAARNGVEGID
                     DVDAAGRLLYPSNVYKSYAFPWLIDGEFDRLTRWLQQLTSHDLSSVDAAG"
     gene            404..1072
                     /locus_tag="VFX21_10185"
     CDS             404..1072
                     /locus_tag="VFX21_10185"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX5096375.1"
                     /translation="MRTDVRAFLVASSGERAAHYVRQLIFDGELRPGDRVPQDRIATD
                     LGMSKIPVREALVALEREGWVTLEPYRGVFVIAVDEEVVRDQFQLFALLFGFAARRCI
                     EHDDAALLGELTRMYDALAQSSDPAEIDSIVYAFHWAIADGARSARLTAVLRGMSAYF
                     PGTFFSLSGASDAIERREMGALLDAMRERKADAAVATYERLMYDLTDIAIKKLRDRGL
                     FEEG"
     gene            1084..>2028
                     /locus_tag="VFX21_10190"
     CDS             1084..>2028
                     /locus_tag="VFX21_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5096376.1"
                     /translation="MAVARQAAPIKFGVLTDMVIPKTPERDVWEDMVRPFELVFREGF
                     EQGVLDRPVELVRREVEGLPRGTVKAVIDAYGELADEGCLAVFGPNISENTIPLRAEI
                     ERRFQVPAISLCGADDWLGEWTFALPAGSMTDEPILIAHLIARAGLQTAGVLVERSLI
                     GQLYLRGFRDACRDAGIALVAEETIAQIDQDIEGAVRAIHKAKPDALVHLGFGFGVIR
                     INEALRSCDWDPPRYMGTSFEDAYFSHEIWDAFVGWIGLEQYDEGNPVGQRFLDRYEA
                     VYGDRPQYFAPVVSRDVAVSFLRAFADAQPLSPRGVMEA"
BASE COUNT          319 a          653 c          732 g          324 t
ORIGIN      
        1 cccggcggcg tcgaccgatg agagatcgtg ggaagtgagc tgctggagcc aacgcgtgag
       61 ccggtcgaac tcgccgtcga tcagccaggg gaacgcgtac gacttgtaga cgttcgacgg
      121 gtagagcagc cggccggccg cgtcgacgtc gtcgatgccc tcgacgccgt tgcgcgccgc
      181 gagacggtcg agcagctgca cgcgcggtcg caaggtgtcg aaccggtagc gcagcgcgtc
      241 gcgctgcagc tcgcggatct ccgcgtacgg gacggcgaac atcttcgacg cggtcccgaa
      301 cagggcgcgc ggatcgtcgg cgaggagtcg ggcgtcgtcg atcggtgatc ctcctggcgc
      361 cggtcgatgg tgcggccaga tctcataaca tatattcaga gcgatgcgaa ccgacgtgcg
      421 ggccttcctg gtcgccagca gcggtgagcg cgccgcgcac tacgtgcggc agctcatctt
      481 cgacggtgag ctgcgacccg gcgaccgcgt gccccaggac cgcatcgcca ccgatctcgg
      541 gatgagcaag atcccggtgc gcgaagcgct ggtcgcgctc gagcgcgagg ggtgggtcac
      601 gctcgaaccc taccgcggcg tcttcgtgat cgcagtcgac gaagaggtgg tgcgtgatca
      661 gttccaattg ttcgcgttgc tgttcgggtt cgcggcccgg cgctgcatcg aacatgacga
      721 cgcggcgctg ctcggcgagc tcacgcggat gtacgacgcg ttggcgcaga gcagcgatcc
      781 cgcggagatc gacagcatcg tgtacgcgtt ccactgggcg atcgccgacg gcgctcggtc
      841 cgcgagactg accgcggtgc tgcggggcat gtcggcgtac ttccccggaa cgttcttctc
      901 gctgtccggc gcgagcgacg ccatcgagcg acgcgagatg ggcgcgctgc tcgacgcaat
      961 gcgcgagcgc aaagccgacg ccgcggtcgc gacgtacgag cggttgatgt acgacctcac
     1021 cgacatcgcg atcaagaagc ttcgcgaccg cggcctgttc gaagaaggtt gagggggagt
     1081 cacatggcag tcgcacggca ggcagcaccg atcaagttcg gtgtgctcac cgacatggtg
     1141 atcccgaaga cgcccgagcg cgacgtctgg gaggacatgg tccggccgtt cgagctcgtg
     1201 ttccgcgagg gattcgagca gggtgtgctc gatcgcccgg tcgagctcgt gcggcgcgag
     1261 gtggagggcc tgccgcgggg cacggtgaag gcggtgatcg acgcctacgg cgagttggcc
     1321 gacgagggat gcctggcggt gttcggcccg aacatctccg agaacacgat cccgctccgc
     1381 gccgagatcg aacggcggtt ccaggtgccg gccatcagcc tgtgcggtgc cgacgactgg
     1441 ctcggggagt ggacgttcgc gctgccggcc ggttcgatga ccgacgagcc gatcctcatc
     1501 gcgcacctca tcgcgcgggc gggactccag accgcgggcg tgctcgtgga gcggtcgctc
     1561 atcggtcagc tgtacctccg cggcttccgc gacgcgtgcc gcgacgccgg gatcgcgctc
     1621 gtggccgagg agacgatcgc gcagatcgac caggacatcg agggcgcggt gcgcgcgatc
     1681 cacaaggcga agcccgacgc actcgtccac ctcggcttcg gcttcggcgt gattcgcatc
     1741 aacgaagcgc tccgctcgtg tgactgggac ccgccccggt acatgggcac ctccttcgag
     1801 gacgcctact tcagtcacga gatctgggac gcgttcgtgg gctggatcgg cctcgagcag
     1861 tacgacgagg gcaaccccgt gggccagcgc ttcctcgacc ggtacgaggc ggtgtacggc
     1921 gatcgcccgc agtacttcgc gccggtggtg tcgcgcgacg tcgcggtgtc gttcctccgg
     1981 gcgttcgccg acgcgcagcc actctctccc cgaggggtga tggaagcg
//
LOCUS       DASUGA010000517         3593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1738546, whole genome shotgun sequence.
ACCESSION   DASUGA010000517 DASUGA010000000
VERSION     DASUGA010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738546"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VFX21_10195"
     CDS             complement(<1..437)
                     /locus_tag="VFX21_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5096377.1"
                     /translation="MTTTSRARPARLVLVEDDRSLRNAMETALRESGYEVMAAGDGRA
                     FDDVLSSFRPDLAVLDVRLPDGPDGFDLAESMRAVTDAPVLFVTAADSLEERLRGFQV
                     GADDYLVKPFALSELLARIKALLRRAGRLTSPTWQVRDVVIDEA"
     gene            558..1619
                     /locus_tag="VFX21_10200"
     CDS             558..1619
                     /locus_tag="VFX21_10200"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5096378.1"
                     /translation="MRVGVIGLGQIAELCLPPYARRDDVELVALCDLDDARLERWRAV
                     FPKALVTHEIDDVLAAQPDVVDILVPTPLHGDVATRVLAAGVDVQVQKPLARSLEDAD
                     RMLAAAAQSGATIRVLEDYMFYPPLVKLREVVTSAEIGAPTGMHMKIVGTGRGGWEVP
                     LSSYIWQFEQSRDGRGMLTFDHGWHQLAIAHWLFGPITRIFGWIGESQAAPDLAPEIM
                     IDAPATFVWEHANGVRATLEILLASETLFRSDFYSCDERVEVTGAQGYVRCNRISSKG
                     IQEPSVVVYREGVMREYHALDDDPPDAFAASAAHAVDYFDHGKGPLVMDGAAARAVLV
                     ALLAGLESSKAGRPVDIHP"
     gene            1785..2240
                     /locus_tag="VFX21_10205"
     CDS             1785..2240
                     /locus_tag="VFX21_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096379.1"
                     /translation="MADSPRFAHLDDLEWEECRRQRHGDRIVSVREKWLDFSPRFLSL
                     YAQWDPGMIVQPHGHNSHHIVFVLKGEMTCGDVVCNAGTHIALDQGDTFGPFVAGPEG
                     VELFEVMMGDPRSFPADLEGYERFLAERGVEQLPNPPIALPEWLEDTRT"
     gene            2329..2691
                     /locus_tag="VFX21_10210"
     CDS             2329..2691
                     /locus_tag="VFX21_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX5096380.1"
                     /translation="MKYMLLIYGNQELWESYQQDDFERVVKETNELQAELRASGEFIG
                     AYGVADQEMAKTVHLADGVPIVTDGPYIETKEYLGSFDIIDCDSLERALEIAARVPFA
                     RVGKVEVRPLMHEAPDNL"
     gene            2688..>3593
                     /locus_tag="VFX21_10215"
     CDS             2688..>3593
                     /locus_tag="VFX21_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEX5096381.1"
                     /translation="MTASPRVEDLLRPLAPQVLGALVRRYGRFGECEDAVQEALVAAA
                     VQWPREGVPDNPRAWLVTVASRRLVDARRSDEARRRREEATGGAPAGEVSGEDDSLTL
                     LMLCCHPALSPASQIALTLRAVGGLTTAEIAHAFLVPESTMAQRISRAKQTIKKVGAT
                     FSRPPPGERADRMRAVLHVLYLVFNEGYVASAGDDLARTDLSNEAIRLTRAMHAVVPD
                     DGEVAGLLALMLLDDARRPARTRADGSLVPLAEQDRTRWDAALVAEGVALITATLPAG
                     PIGPYQIQAAIAAVHDEAPTAGDTDW"
BASE COUNT          569 a         1230 c         1253 g          541 t
ORIGIN      
        1 gcttcgtcga tgacgacgtc gcgaacctgc caggtcggtg acgtgagccg gccggcccga
       61 cgcagcagcg ccttgatgcg agcgagcaac tccgagagtg cgaacggctt gacgaggtag
      121 tcgtcggcgc cgacctggaa gccgcgcagt cgttcttcga ggctgtcagc cgcagtcacg
      181 aagaggacgg gcgcgtcggt gacggcgcgc atcgactccg cgaggtcgaa gccgtcgggc
      241 ccgtcgggca gccgcacgtc gagcacggcc aggtcgggcc ggaacgacga cagcacatcg
      301 tcgaacgcgc ggccgtcgcc ggccgccatc acctcgtagc ccgactcccg cagcgcggtc
      361 tccatcgcgt tgcgcagcga ccgatcgtct tcgacgagca cgagacgcgc cggtcgtgct
      421 cgggatgtgg tcgtcatcgc gtcagacatc atcgacattg tgcgggccgg attcggcgcg
      481 tcgggcgaaa cagtcgttct cctcagcaaa cttcagcaga actcgcgacg ctctgtcgag
      541 gctgcaagac tccggcgatg cgcgtcggtg tcatcgggct ggggcagatc gcggagctgt
      601 gtctgcctcc gtacgcacga cgggacgacg tggaactcgt cgccttgtgc gacctcgacg
      661 acgcgcgact cgaacggtgg cgcgccgtgt tcccgaaggc cctcgtgacg cacgagatcg
      721 acgacgtgct cgccgcccag ccggacgtgg tcgacatcct cgtgcccacg ccgttgcacg
      781 gcgacgtcgc gacgcgcgtc ctggcggccg gcgtcgacgt ccaggtccaa aagccgttgg
      841 cgcgcagcct cgaggacgcc gaccggatgc tggccgcggc cgcacagtcg ggcgcgacca
      901 ttcgggtgct cgaggactac atgttctatc cgccgctggt gaagctgcgc gaggtcgtca
      961 cgtcggcgga gatcggcgcg ccgaccggca tgcacatgaa gatcgtcggc acgggacggg
     1021 gcggttggga agtgccgctg tcgagctaca tctggcaatt cgaacagtcg cgtgacgggc
     1081 gcgggatgct gacgttcgac cacggttggc accagctcgc gatcgcgcac tggttgttcg
     1141 ggccgatcac gcgcatcttc ggctggatcg gcgagtcgca ggccgcgccc gatctcgcgc
     1201 ccgagatcat gatcgacgcg ccggccacgt tcgtctggga gcacgccaac ggtgtgcgcg
     1261 cgacgctcga gatcctgctc gcgtcggaga cgttgttccg ctccgacttc tacagctgcg
     1321 acgagcgcgt cgaggtgacc ggcgcgcagg ggtacgtgcg ctgcaaccgc atcagctcga
     1381 agggcatcca ggaaccgtcg gtcgtcgtgt accgcgaggg cgtgatgcgc gagtaccacg
     1441 cgctcgacga cgacccgccg gacgcgttcg cggcgtcggc cgcgcacgca gtcgactact
     1501 tcgaccacgg caagggcccg ctcgtgatgg acggcgcggc ggcccgggcc gtgctcgtcg
     1561 cgctgctcgc cggcctcgaa tcgtcgaagg cgggacggcc ggtcgacatc catccctgag
     1621 cgcgcgtctc gcgaccagct gccctggggt cgctcccatt cctgaatcgt catcagattg
     1681 caagaccggc cggtatgtgg ccgcttctgc aatctgatgg cagaagcggc cggtgcacac
     1741 gccgtcgtta cgatgcgcgg cgacacccgc ggcaaggaga cggcatggcg gacagcccgc
     1801 gtttcgcaca cctcgacgat ctcgaatggg aagagtgccg ccgccagcgc cacggcgacc
     1861 gcatcgtgtc cgtccgtgag aagtggctcg acttctcgcc gcggttcttg tcgctgtacg
     1921 cgcagtggga cccgggcatg atcgtgcagc cgcacggcca caacagccac cacatcgtgt
     1981 tcgtgctcaa gggcgaaatg acgtgcggcg acgtcgtgtg caacgccggc acgcacatcg
     2041 cgctcgacca gggcgacacg ttcgggccgt tcgtcgccgg ccccgagggc gtcgagctgt
     2101 tcgaggtcat gatgggcgac ccgcgctcgt tcccggccga cctcgaaggc tacgaacgct
     2161 tcctcgccga acggggcgtc gagcagctcc cgaacccgcc gatcgcgctg cccgaatggc
     2221 tcgaggacac ccggacatga ccttcgtccg aaatatcggt caccgatgtc gatcgcgtga
     2281 cttcggctcc gactcaccac cgcagcccgc gatcatccgg aggaaaccat gaagtacatg
     2341 ctgctcatct acggcaacca ggagctgtgg gagtcgtacc agcaggacga cttcgaacgg
     2401 gtggtcaagg agaccaacga gctgcaggcc gagctgcgcg cctcgggcga gttcatcggt
     2461 gcctacggcg tcgcggacca ggagatggcc aagaccgtgc acctcgccga cggcgtgccg
     2521 atcgtcaccg acggtcccta catcgagacc aaggagtacc tcggcagctt cgacatcatc
     2581 gactgtgaca gcctcgagcg cgcgctcgag atcgcagctc gggtgccgtt cgcacgggtc
     2641 ggcaaggtcg aggtgcggcc gttgatgcac gaggcgcccg acaacttgtg acggcgtcgc
     2701 cgcgcgtcga agacctgctg cgaccgttgg cgccgcaggt cctcggcgcg ctcgtgcggc
     2761 ggtacggtcg cttcggcgag tgcgaggacg ccgtgcagga ggcgctcgtc gcggccgcgg
     2821 tgcagtggcc ccgagaaggt gtgcccgaca atccgcgcgc gtggctggtc acggtggcct
     2881 cgcggcggct cgtcgacgcg cgccgcagcg acgaagcgcg ccgccgtcgg gaggaggcaa
     2941 cgggcggcgc gccggccggc gaggtcagcg gcgaggacga ctcgctcacg ttgctcatgc
     3001 tctgctgcca tcccgccttg tcgccggcat cgcagatcgc gctcacgttg cgggccgtcg
     3061 gtggcttgac caccgcggag atcgcgcacg cgttcctcgt gccggaatcg acgatggcgc
     3121 agcgcatcag ccgcgccaag cagacgatca agaaggtggg tgcgacgttc tcgcggccgc
     3181 cgcccggcga gcgcgcggac cggatgcgcg cggtactgca cgtcctctat ctcgtcttca
     3241 acgaaggcta cgtcgcgagt gccggcgacg acctcgcgcg caccgatctc tcgaacgaag
     3301 cgattcgcct gacgcgcgcg atgcatgccg tcgtgcccga cgacggcgag gtcgccggtc
     3361 tgctcgcgct gatgctgctc gacgacgccc gccgcccggc gcgcacgcgt gccgacggca
     3421 gcctggtgcc gctcgccgag caggaccgca cgcggtggga cgcggcgctc gtcgccgaag
     3481 gcgtcgcgtt gatcacggcc acgctgcccg ccgggccgat cggtccgtac cagatccaag
     3541 ccgcgatcgc ggcggtgcac gacgaagcgc cgacggccgg tgacaccgac tgg
//
LOCUS       DASUGA010000518         3252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1477118, whole genome shotgun sequence.
ACCESSION   DASUGA010000518 DASUGA010000000
VERSION     DASUGA010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477118"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VFX21_10220"
     CDS             <1..504
                     /locus_tag="VFX21_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096382.1"
                     /translation="TPDGGGYWMFGADGAVYAFGSAGFFGRLDPSAGGAPIVAATASP
                     TGNGYWLFGADGGVYAFGDAGFYGSMGGAALNGAVTAAAATPSGNGYWLFGADGGAFS
                     FGDAVFHGSMGGTVLNGTVVAALSTATGDGYWLFGADGGVFSFGAAGFFGSLGGIPLR
                     SAVVTAT"
     gene            complement(522..1796)
                     /gene="efeB"
                     /locus_tag="VFX21_10225"
     CDS             complement(522..1796)
                     /gene="efeB"
                     /locus_tag="VFX21_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HEX5096383.1"
                     /translation="MARASQRFDPSRRRFLTGAASASALAIGAPVLLNACGSDDDDTA
                     ARNSAFPGGERVPFHGVHQSGVLQAAPAAGIVVALDSQAKDRAELDEAFRALSDETRR
                     VMDGTAPEERDPSFPPSDSGVLGDVSHPAALSVIVGVGASLFDERYGLASRKPRELEK
                     MPFVANDRLDPARSHGDISLTVSASSADVAMYALRQLLRKTRGAFTLRWMQEGFNTVQ
                     DQPGPGKAAVRNLLGFKDGSNNLDPASATTMDKLVWVDASRGEPAWAVNGSYQAIRII
                     RMFVEFWDRTRLSEQEALIGRHKASGAPLGGDKEDEPFDFADDPHGDRIPLDAHIRLA
                     NPRTPETEKNRILRRGFSYSRGFDGAGHLDQGLLFSSFQASLTDGFLAVQRRLAGEPL
                     EEYILPEGGGFFYVLPGAKDDRSYLGQSLLAP"
     gene            complement(1814..2746)
                     /gene="efeO"
                     /locus_tag="VFX21_10230"
     CDS             complement(1814..2746)
                     /gene="efeO"
                     /locus_tag="VFX21_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEX5096384.1"
                     /translation="MRKRWVAVFVLCALALAACSDDGNDTRSGSASGSGSGSGSGSAS
                     GGGSGSGSGISASDVEGTTDDEAILEATAAYKQYVEQQVDELVTKTTTFTDAVRAGDL
                     AAAQAAYAPSREPWERIEPIAGLIEDIDGAVDARVDDFEGEDDPAWTGWHRLEYLLFA
                     QNTTEGATEFADKLDADLAKLQSSLANLEMPPAALAVGAAELIEEVSEGKITGEEDRY
                     SHTDLWDFAANVEGSKHLITLLTPALEAKDPELLGQINEQFAEVEAELAPYEDGDGYK
                     SYEELTDADKEAMTASLAALSESLSQVAGTLGLE"
     gene            complement(2743..>3252)
                     /locus_tag="VFX21_10235"
     CDS             complement(2743..>3252)
                     /locus_tag="VFX21_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096385.1"
                     /translation="QPAFLLAIPAVVLLLRDRPRHWLVLVAPLAVGWLNATFVALTMH
                     GWWWPGRQTVVVLPCLVLAVTWFAASRPVVLRALALLGLFGATVFAWLVAQATFGGIN
                     TVVTFDHISYPLVRGWQLLLPDYRAHTPRDWVLHGAWLAAVAACVLATWRPHFAMKQR
                     FTMTLKETS"
BASE COUNT          467 a         1152 c         1124 g          509 t
ORIGIN      
        1 acgcccgacg gcggcggcta ttggatgttc ggcgccgacg gcgccgtcta tgcattcggc
       61 agtgccgggt tcttcggccg gctcgacccg agcgcgggtg gcgcgccgat cgttgccgcg
      121 acggcttcgc cgaccggcaa cggctactgg ctcttcggcg ccgacggcgg cgtctacgca
      181 ttcggcgacg cgggcttcta cgggtcgatg ggcggcgcgg cactgaacgg cgccgtcacc
      241 gccgcggccg cgacgccgtc cggcaacggc tactggctct tcggcgccga cggcggcgcg
      301 ttctcgttcg gtgacgcggt cttccacggg tcgatgggcg gcacggtgct caacggaacc
      361 gtcgtcgccg cgctctcgac cgctaccggc gacggctact ggctgttcgg cgccgacggt
      421 ggtgtcttca gcttcggcgc cgccgggttc ttcggctccc tgggcggcat cccgctccgt
      481 tccgccgtcg tcacggcaac ctgaccgggc gccggacagg tctacggcgc gaggagcgac
      541 tggccgaggt aggaccggtc gtccttcgcg cccggcagca cgtagaagaa gccgccgccc
      601 tcggggagga tgtactcctc gagtggctcg ccggccagcc gccgctgcac cgcgaggaac
      661 ccgtcggtca ggctggcctg gaacgacgag aacagcaggc cctgatcgag gtggcctgcc
      721 ccgtcgaagc cgcgcgagta gctgaagccg cggcgcagga tccggttctt ctcggtctcg
      781 ggggtgcgcg ggttggcgag gcggatgtgc gcgtcgagcg ggatgcggtc cccgtgcgga
      841 tcgtcggcga agtcgaacgg ttcgtcttcc ttgtcgccac cgagcggcgc gccgctcgcc
      901 ttgtggcgac cgatgagcgc ctcctgctcg gacaggcgcg tgcgatccca gaactcgacg
      961 aacatccgga tgatgcggat cgcctgatag gagccgttca ccgcccacgc cggctcaccg
     1021 cggctcgcgt cgacccacac cagcttgtcc atcgtcgtcg cgctcgcggg atcgaggttg
     1081 ttcgatccgt ccttgaaccc caggaggttg cggaccgcgg ccttccccgg gccgggctgg
     1141 tcctgcaccg tgttgaagcc ttcttgcatc caccgcaacg tgaacgcgcc gcgcgtcttg
     1201 cgcagcagct gacgcaacgc gtacatcgcg acgtccgcgc tcgaggcgga cacggtgagc
     1261 gagatgtcgc cgtggctgcg cgccggatcg agacggtcgt tcgcgacgaa cggcatcttc
     1321 tcgagttcgc gcggcttgcg gctcgcgagc ccgtaccgct cgtcgaacag cgacgcgccg
     1381 acaccgacga tcacgctcaa cgccgccgga tgcgacacgt cgccgagcac acccgagtcg
     1441 gacggcggga acgacgggtc gcgctcttcg ggtgccgtgc cgtccatcac acgccgggtc
     1501 tcgtcggaca gcgcgcggaa cgcttcgtcc aactcggctc ggtccttggc ctggctgtcg
     1561 agcgcgacga cgatgccggc ggccggtgcc gcctgcagca cgccgctctg gtgcacaccg
     1621 tggaacggca cgcgctcacc accggggaac gcggagttgc gcgccgcggt gtcgtcgtcg
     1681 tcactgccgc aggcgttgag gagcacgggc gcgccgatcg cgagcgccga ggccgacgcc
     1741 gctcccgtga ggaagcggcg ccggctcgga tcgaatcgct gcgatgcgcg tgccaacggt
     1801 ccgtgctgct ccgttactcg agaccgagcg ttcccgcgac ctgcgacagg ctctcggaga
     1861 gcgccgccag cgacgcggtc atcgcttcct tgtcggcgtc ggtcaactct tcgtacgact
     1921 tgtagccgtc gccgtcttcg tagggcgcca gctcggcttc gacttcggcg aactgctcgt
     1981 tgatctgtcc gagcagctcg gggtccttcg cttcgagcgc gggcgtgagc aacgtgatca
     2041 ggtgcttcga gccctcgacg ttcgcggcga agtcccagag gtcggtgtgc gagtagcggt
     2101 cctcttcacc ggtgatcttg ccctccgaca cctcttcgat cagctcggcg gccccgacgg
     2161 ccagcgcggc cggcggcatc tcgaggttcg cgagcgacga ctgcaacttg gcgaggtcgg
     2221 cgtcgagctt gtcggcgaac tccgtcgcac cttcggtcgt gttctgcgcg aacagcaggt
     2281 actccaggcg gtgccaaccc gtccatgccg ggtcgtcctc gccttcgaag tcgtcgacgc
     2341 gcgcgtcgac cgcgccgtcg atgtcctcga tcaagcccgc aatcggttcg atgcgttccc
     2401 agggctcacg cgacggtgcg tatgccgcct gcgccgccgc gaggtcgccc gcccgcaccg
     2461 cgtcggtgaa ggtcgtcgtc ttggtcacca actcgtcgac ctgttgctcg acgtactgct
     2521 tgtacgccgc ggtcgcttcg aggatcgcct cgtcatcggt cgtgccctcg acgtcggacg
     2581 cggagatgcc cgaacccgaa ccggagccgc cacccgacgc gctgcccgag cccgagccgc
     2641 tgcccgaacc ggacgcgctt cccgaacgcg tgtcgttgcc gtcgtcgctg cacgccgcga
     2701 gtgcgagcgc gcacaacacg aacaccgcca cccaacgctt tctcacgagg tctccttcag
     2761 tgtcatggtg aaccgttgct tcattgcgaa gtgcggccgc caggtcgcga gcacgcaggc
     2821 cgcgacggcg gccagccacg cgccatggag gacccagtcg cgcggcgtgt gcgcgcggta
     2881 gtcgggcagc aggagctgcc aaccgcgcac gagcggatac gagatgtggt cgaacgtgac
     2941 gaccgtgttg atgccaccga aggtcgcttg cgcgacgagc caggcaaaga ccgtcgcgcc
     3001 gaacaatccg agcagcgcga gcgcgcgcag gacgacggga cgcgacgccg cgaaccacgt
     3061 cacggcgagc acgaggcacg gcagcacgac gaccgtctgg cgtcccggcc accaccaccc
     3121 gtgcatcgtc aacgcgacga aggtcgcgtt cagccacccg accgcgagcg gcgcgacgag
     3181 cacgagccag tgccggggcc ggtcccgcag caacaggacg acggccggga tcgcgaggag
     3241 aaacgcgggc tg
//
LOCUS       DASUGA010000519         4827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_60980, whole genome shotgun sequence.
ACCESSION   DASUGA010000519 DASUGA010000000
VERSION     DASUGA010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60980"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1204)
                     /locus_tag="VFX21_10240"
     CDS             complement(47..1204)
                     /locus_tag="VFX21_10240"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX5096386.1"
                     /translation="MRVRRLVVIGLGAAMLLACSGCVRVDLQRLASDAADGRDNGTAG
                     SVLAQDYILDYLRSWTSGPNAGTGDERYKQAFAGGTNLIGVLPGTDLADEYVLVGAHF
                     DHVGHGCEDLRPNDDICNGATDNAAGVVAVLDVLRKMYYGGDVRRSVIFAFWDREEDG
                     LLGARHYAQNPIIPLADTVAYVNLDIQGSNVRPSLRNVSFAVGAETGGPRMRQLVSDA
                     TAPGQLGLGQLSVIFGQGRSDHVPFIAAGVPSVFFTDATGPCYHTDSDDIDVVDFAKL
                     DQQIGAVLRLTRALAETDDLPTFTTGLPIATYDDAAILYVVSSVLVMHDLDTFNQLQA
                     DFIVDAHAQLQQIILDGPEAFDSTDASTMLQNAAAIVSFLATGPCDGYLTP"
     gene            complement(1325..2077)
                     /locus_tag="VFX21_10245"
     CDS             complement(1325..2077)
                     /locus_tag="VFX21_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096387.1"
                     /translation="MNASACRRVAVLGFVLALGGAACGGDDDGAAAAPVIDPGDGGHY
                     APEIDPANFVTAVDNPYFPLPPGARWEYEGSEDGEAQTDVVEVTDEQRTVMGVPVVVV
                     RDTVSAKGVPTEDTRDWYAQDAEGNVWYFGEDVRNFENGKLQSSDGSWEAGVDGALPG
                     IIMPAHPKVGDAYRQEYDKGKAEDLGKILRVGRHKDVTAGSYDNVIVTRDWNPLEPDV
                     IEEKSYAPGVGVVYERTVQGGDETSQLTSMTE"
     gene            complement(2079..2822)
                     /locus_tag="VFX21_10250"
     CDS             complement(2079..2822)
                     /locus_tag="VFX21_10250"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5096388.1"
                     /translation="MTDTLLTPTRPVVEAARSAPCLHCHPPATPSSFYDRRRPLLAGL
                     GLAFVLLAAAAALAGGWLALRWDLPIARFAIAHRSDTLDTFFRSASRLGSTVVVLGLS
                     AVLVLVTWSRCRAVALAIAVAALTRPLIEFVLKDAVDRPRPSLSRLVVGTGPSFPSGH
                     VMAAVALYGLLPLVVGLFTRNRVVWWASAAVSGVLIVTIAASRVYLGVHWFSDVVGSM
                     LLGSFFLLAVEAIHRSLHRSMPHEHSCPP"
     gene            2930..4018
                     /locus_tag="VFX21_10255"
     CDS             2930..4018
                     /locus_tag="VFX21_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5096389.1"
                     /translation="MIVAGELREPRLRHRARLWSGRARLTFGKTQRAMTRSLSRAYSR
                     VGRSPVAALSGSFREVPADRLLLGDAASTDDLLGASTPLPDWPADLATESATVPHVRP
                     IRHSDCFFVVDGHEQLRDAYTQGRSALRVRVGRVATWTPLQEELLGMNWLEGRRELYQ
                     PVDAPEIEHGDWQLVRRCDDRFALMRDFLRDHDLLSPQTNTYLDVGASYGWFVGRMQR
                     LGLDAYGIELDERGPRLAQSLYGVPADHIRVGDCATELCTIEPVDVVSCLSVMHHFVL
                     GRGSCSADEFLDRLARVTQRVLFFDTGEGHELWLRRRLADWTPGRIEEWLAAHPAFTE
                     VHNLGTDSDDTGSFAGNYGRTLFACVRR"
     gene            4063..4743
                     /locus_tag="VFX21_10260"
     CDS             4063..4743
                     /locus_tag="VFX21_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5096390.1"
                     /translation="MARVLVVEDEVRVAGAVRRGLEGEGFAVDVARDGEEGLWLAREQ
                     PYDVIVLDVMLPKLNGYRVCATLREEGNWTPILMLTAKTGEYDEAEALDTGADDFLSK
                     PFSYVVLVARLRALLRRGARARPTVLEGAGLKLDPAAHRVWRGDEEIVLTAREFAVLE
                     FLLRRAGQTVSKSEILASVWDFEFEGDPNIVEVYLRRLRRKIDEPFDTKSIVTIRGAG
                     YRLEAAHG"
BASE COUNT          790 a         1608 c         1674 g          755 t
ORIGIN      
        1 cgacggctga aacgcgttga ccccgggcaa tcagtgcggg cgatcctcag ggagtcaggt
       61 atccgtcgca cggccccgtt gcgaggaagc tgacgatggc cgcggcgttc tgcagcatcg
      121 tcgatgcatc ggtgctgtcg aacgcctccg gtccgtcgag gatgatctgt tgtagctgtg
      181 cgtgagcatc gacgatgaag tcggcttgga gctggttgaa cgtatcgagg tcgtgcatca
      241 ccagcactga ggacacgacg tagaggatcg ccgcatcgtc gtaggtcgcg atcggcagcc
      301 cggtcgtgaa cgtcggcagg tcgtcggtct cggccagcgc tcgcgtcagg cggagtacgg
      361 caccgatttg ctggtcgagc ttggcgaagt cgacgacgtc gatgtcgtcc gagtcggtgt
      421 ggtagcaggg gcccgtcgcg tccgtgaaga acacgctggg aacgcccgcg gcgatgaacg
      481 gcacgtggtc gctgcgtcct tgcccgaaga tgacactcag ctgtccgaga ccgagctgac
      541 ccggtgcagt cgcgtccgag acgagttggc gcatgcgcgg gccaccggtc tcggcgccga
      601 ccgcgaagct cacgttgcga aggctcgggc ggacgttcga gccctgaatg tcgagattga
      661 cgtacgcgac cgtgtccgcg agcgggatga tcgggttctg cgcgtagtgg cgcgcgccaa
      721 ggagaccgtc ctcttcacga tcccagaacg cgaagatgac cgagcggcgc acgtctcctc
      781 cgtagtacat cttgcgcagc acatcgagca ccgcgacgac accggccgcg ttgtcggtcg
      841 caccgttgca gatgtcgtca ttcgggcgga gatcctcaca gccgtgtccg acgtggtcga
      901 agtgggcacc gacgagcacg tattcgtcgg caagatccgt tccgggcaac acgccgatca
      961 agttcgtgcc gccggcgaag gcctgcttgt accgctcgtc gccggtgccg gcgttcggtc
     1021 ccgacgtcca ggagcggaga tagtcgagga tgtagtcctg cgcgagaacg gacccggccg
     1081 tgccgttgtc gcggccatcg gcggcgtccg atgcgagccg ctggaggtcc acgcgcacac
     1141 atcccgagca tgccagcagc atcgctgctc ccaaaccgat gacgacgagc cgacggacgc
     1201 gcatcgaaga cccccttcgc ggcatgctgc gatggccgca cggtacgacg attggacgcc
     1261 ttccccaagc atcggacgcg ttgtccggac gggggacgac caagggaagg acgccgcgcc
     1321 ggactcactc tgtcatcgat gtcagctgtg atgtctcgtc gccgccttgg acggtcctct
     1381 cgtagaccac gccgacgccc ggcgcgtacg acttctcctc gatgacgtcg ggttcgagcg
     1441 ggttccagtc gcgtgtgacg atcacgttgt cgtacgaccc ggctgtgacg tccttgtgcc
     1501 ggccgacgcg gaggatcttg cccagatcct cggccttgcc cttgtcgtac tcctgccggt
     1561 aggcgtcgcc gaccttcggg tgcgcgggca tgatgatccc gggcagggcg ccgtcgacgc
     1621 cggcttccca cgagccgtcg ctgctttgca gcttgccgtt ctcgaagttg cggacgtctt
     1681 caccgaagta ccagacgttc ccctcggcat cctgggcgta ccagtcgcgc gtgtcctcgg
     1741 tcggaacgcc cttcgccgac accgtgtcgc ggacgacgac gaccggaaca cccatgacgg
     1801 tgcgctgctc gtcggtgacc tcgacgacgt ccgtctgcgc ttcgccgtcc tcggaacctt
     1861 cgtactccca ccgcgcgccg ggcggaagcg ggaagtacgg attgtcgact gcggtcacga
     1921 agttcgcagg atcgatctcg ggcgcatagt gaccgccgtc gccgggatcg atgaccggcg
     1981 ccgcggccgc accatcgtcg tcgccgccgc aggccgcacc cccgagcgcg aggacgaacc
     2041 cgagcaccgc gacgcggcga cacgccgacg cgttcatctc acggcgggca cgagtgctcg
     2101 tgcggcatcg atctgtgcaa cgagcgatgg atcgcttcta ccgcgagcaa gaagaacgaa
     2161 ccgagcaaca tgctgccgac gacgtcggag aaccagtgca cgccgagata cacacggctt
     2221 gccgcgatcg tgacgatcag tacgcccgag actgccgccg aggcccacca cacgacgcga
     2281 ttgcgcgtga agaggccgac gacgagcggc aacaatccgt agagcgcgac cgcagccatg
     2341 acgtgcccgc tcgggaacga cggacccgtc cctacgacga gacgagacaa gctcgggcgc
     2401 ggacgatcga cggcgtcctt caggacgaac tcgatcagcg gacgggtcaa cgccgcgacc
     2461 gcgatcgcca acgcgaccgc gcgacagcgc gaccacgtga cgaggacgag gacggcactc
     2521 agccccagga cgacgaccgt cgatccgagg cgcgatgccg atcggaagaa ggtgtcgagc
     2581 gtgtcgctgc gatgcgcaat cgcgaagcgg gcgatcggaa gatcccagcg cagggcgagc
     2641 cagccgccgg cgagcgcggc cgccgccgcg aggagcacga atgcgagacc cagaccggcc
     2701 aggagcggcc gccgccggtc gtagaacgac gacggcgtcg cgggcgggtg gcagtgaaga
     2761 cagggcgccg accgtgcagc ctcgaccacg gggcgcgtcg gcgtgagcag ggtgtccgtc
     2821 atgcccgttc agtgtgcgcc ggagtcgctg acaggaggct gaacggcacg cagcgcgcct
     2881 tcatgtgcgg ttcagcggcg gtacagggaa cgggccggat actggcccca tgatcgttgc
     2941 cggcgagttg cgagagccgc gcctgcggca ccgagcccga ttgtggtcgg gccgcgcccg
     3001 gctcacgttc ggaaagaccc agcgtgcgat gacccgttcg ctgtcacggg cgtattcccg
     3061 tgtcggccgc agcccggtcg cggcgctgtc ggggtcgttc cgggaagtcc ccgccgaccg
     3121 gctactcctc ggcgacgcgg catccaccga cgatctgctg ggcgcctcga cgccgctgcc
     3181 cgattggcct gccgatctcg caaccgagtc cgccaccgtc ccgcacgtcc gtccgatccg
     3241 ccattccgac tgcttcttcg tcgtcgacgg ccacgaacaa ctgcgcgacg cgtacacgca
     3301 gggccggagt gcgctgcgcg tgcgcgtcgg tcgcgtcgcg acgtggacgc cgctgcaaga
     3361 agagctgctc gggatgaact ggctcgaagg gcgccgcgag ctctaccagc cggtcgacgc
     3421 gccggagatc gaacacggcg actggcagct cgttcgccgc tgcgacgacc gcttcgcgtt
     3481 gatgcgcgac ttcctgcgcg accacgacct gctgtcgccg cagacgaaca cctacctcga
     3541 cgtcggcgcg agctacggct ggttcgtcgg acgaatgcag agactcggcc tcgacgccta
     3601 cgggatcgag ctcgacgagc gcgggccgcg actggcacag tcgctctacg gtgtgccggc
     3661 ggaccacatc cgcgtcggtg actgcgcgac cgagttgtgc acgatcgaac ccgtcgacgt
     3721 cgtctcctgc ctgagcgtca tgcatcactt cgtcctcggt cgtgggtcgt gcagcgcgga
     3781 cgagttcctc gaccggctcg cacgcgtgac ccagcgggtc ctcttcttcg acaccggcga
     3841 gggccacgag ctctggctgc ggcgccgact ggccgactgg acgccgggcc gcatcgaaga
     3901 gtggctggcc gcccatcccg ccttcacgga agtgcacaac ctgggcaccg actccgacga
     3961 caccggatcg ttcgccggca actacgggcg aaccctcttc gcctgcgtgc ggcgctgatc
     4021 cgcacgcgcc gggacgcgcg aggctctggt cgaggagggg gcgtggcgcg cgtcctggtg
     4081 gtcgaagacg aggtgcgggt cgcgggtgca gttcggcgcg gcctcgaggg cgaagggttc
     4141 gcggtcgatg tcgcgcggga cggtgaagag ggactctggc tcgcgcgcga acagccgtat
     4201 gacgtcatcg tgctcgacgt catgctgccc aagctgaacg ggtaccgcgt gtgcgcgacc
     4261 cttcgcgaag agggcaactg gacgccgatc ttgatgctga cggccaagac gggagagtac
     4321 gacgaggcgg aagccctcga cacgggcgcg gacgactttc tctcgaagcc cttctcgtac
     4381 gtcgtgctcg tcgcccgatt gcgagcgttg ctccgccgcg gcgcccgggc ccggccgacg
     4441 gtcctggaag gtgcgggcct gaagctcgat cccgccgcgc atcgcgtgtg gcggggcgac
     4501 gaggagatcg tcctgacggc gcgcgagttc gcggtcctgg agttcttgtt gcgccgcgcg
     4561 ggtcagacgg tgagcaagtc ggagatcttg gcctcggtgt gggatttcga gttcgagggc
     4621 gaccccaaca tcgtcgaggt ctacctgcgc cggctccggc gcaagatcga cgaaccgttc
     4681 gacacgaaga gcatcgtgac gattcggggc gcgggatacc gactggaagc cgcgcatggg
     4741 tgagtcgagg cgccgccggt tcgcatcggt ccgggtccgc acgacggccg cggtcacgct
     4801 cgttgtcgcc gccaccctcg tgctcgg
//
LOCUS       DASUGA010000520         6275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_752139, whole genome shotgun sequence.
ACCESSION   DASUGA010000520 DASUGA010000000
VERSION     DASUGA010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6275
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752139"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1328
                     /locus_tag="VFX21_10265"
     CDS             <1..1328
                     /locus_tag="VFX21_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX5096391.1"
                     /translation="GHDGGSPVLLYVVQCTSSNGGVAKTVFSGPEGVVVTGLTNGGLY
                     TCKALGANAIGTGPYSAPSAQFRVSAPPAAPTGVSAAPGSGSAIVSFTPGANGGNPDV
                     AFVVQCTSSNGGVGGGNFFTASPATVTGLTNGKTYTCTVIAAHAQGASAPSSASNAIV
                     IGVPSAPTAVSATPRNGEATVSWTAPANNGSAITNYIVTPYIGAAAQTAQTFNASATS
                     RVVTGLTNSSVYTFKVAAVNARGTGAQSTASNTVTVGTPAAPPAASAVPGAAQATVSW
                     TAPANNGSAITGYQVVPIVNGTPQAPRLFASTATSQVITGLTNATTYTFAVAAVNARG
                     TGLAKTTAAITVGAPTAPPLVTATAGAGKATVTWTAPASNNGAAITNYIVTPFIGGVA
                     QTPVTFNASQLTRQISGLTPGTAYTFTVSAVNSRGTGPASAPSNSATPT"
     gene            1502..2044
                     /locus_tag="VFX21_10270"
     CDS             1502..2044
                     /locus_tag="VFX21_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096392.1"
                     /translation="MSTMWVRRPTAAPAGDVAAPTFKLERPLAGVTVGIRTDTAWRSW
                     RLIAQQWDEWLRRDGAQTVLVETGGQVGAVGADDRKHIVELAQTVDCAIVGLGTCGSC
                     TTFTVKDSVTVERHEKPVVAVVTEEFETHGHNVATHLGHRDLELLVLPYPLEARPEAE
                     LREIADTYYPQVLDLLGVQR"
     gene            2041..3090
                     /locus_tag="VFX21_10275"
     CDS             2041..3090
                     /locus_tag="VFX21_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096393.1"
                     /translation="MTLTSERVEVPSDARAQYELSFTEKWGDGVPLLPATDDAIEALL
                     AATPYPADHVVCVLPPRNGVATAELVAVNAAMAGVEPDAFPLVIAALEALSEPEWNAF
                     GLTTTTSSVFPMLIVNGPCRDELAIDSRASCMGGAAGRGSMTIGRAVALCLRNIGGQR
                     AGETTKSVFGQPARFGFCIGEWEERSPWPSLAQRRGFGADDNVVTVHGGKGTFPIADI
                     HNDDPRDLLYLIAKSLAYPLANMYLGHAANGEVVLAINPMWAERFAQPFPGVEQLQQY
                     LRDNAWQPIDLWPPGNQEILRKKGRVDADGRVHLVARADQIVPIVCGGLGSLHAIGLP
                     SFGESQMQSQAARSA"
     gene            3093..3341
                     /locus_tag="VFX21_10280"
     CDS             3093..3341
                     /locus_tag="VFX21_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096394.1"
                     /translation="MDTDAIAAAVDETGRFLRADGADLVLLDADPKTARVRLQLVLDS
                     VECADCVLPPDALHETIVDALQRRVPGDYEVVVDDPRR"
     gene            3338..3769
                     /locus_tag="VFX21_10285"
     CDS             3338..3769
                     /locus_tag="VFX21_10285"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5096395.1"
                     /translation="MTEQAWSALVTVYQSVLHDVVGALESDAGMDSGVFSALAYVARE
                     GNDALPLSELQRLLHPRYSQPGVSRLVQRMEADGLVVRRTDPDDKRASIVSLTSAGRT
                     RYRKANEVYEAAVGEHFARHLTASEARSLVRILDSVGRRGR"
     gene            complement(3705..4556)
                     /locus_tag="VFX21_10290"
     CDS             complement(3705..4556)
                     /locus_tag="VFX21_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEX5096396.1"
                     /translation="MKRIAAPIRFRWKSATMLVAASLFGLAAFLWPLTTNATGDENLA
                     HSTDAPWLFVIILPLLLGILLAELSEGGLDAKAIAMLGVLVACGALLRAPVTGVTGFT
                     GVFFLIVLGGRVFGRGFGFVQGATTMFASALLTGGVGPWLPFQMLGSAWVGLLAGSLP
                     PMRGRREVAMLAAYGALSGLIYGLVMDMWFWPFTTSGANELHFVPGASVVENLQRFWA
                     FHLTTALGFDVARAAGNAALIFVVGTPVLAALRRASRRAAFEAPVIFLDDRPSPEFER
                     ASVLPTP"
     gene            complement(4553..6157)
                     /locus_tag="VFX21_10295"
     CDS             complement(4553..6157)
                     /locus_tag="VFX21_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014142180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5096397.1"
                     /translation="MIRFDAVTFTYPDAARAAIRGVDLLVPEGEFCLVVGETGSGKST
                     LLRAVNGLVPHFSGGTLAGRVSVNGTSTADAPPRELADVVGVVGQNPAAGFVADTVED
                     ELAYVMENLGVAPAVMRRRVEDVLDLLGIANLRGRALGTLSSGEAQRVAIGGVLTASP
                     RVLVLDEPTSALDPSAAEDVLAMLTRFVHDLGLTVLLAEHRLERVVQYADRIATIDDS
                     GRVDAGAPAAMMASAPVTPPVVALGRLAGWSPLPLSVRDARRAADDLRVRLNGRVPEI
                     GARPAPGEELASASRVKMRYGSVEALHAVDLRIHAGEIIALMGRNGAGKSTLLSLLAG
                     LRPPTAGTVRVAGDAPHTLTPHTLVGRVALVPSDPGSILYERSIADECTVADREHHLA
                     PGTTRAMLDRIHDGLDPEHHPRDLSEGERLALALAVVLAPAPRVLLLDEPTRGLDYTA
                     KARLATVLRGLAADGHAIVLASHDVELVAEVATRAVVLADGDVVSDGPAREVVCHSPV
                     FAPQVAKILAPSEWLTVAEVAAALAS"
BASE COUNT          993 a         2190 c         2272 g          820 t
ORIGIN      
        1 cgggacacga cggcggcagc ccggtgctgc tgtacgtcgt ccagtgcacc tcgtcgaacg
       61 gcggcgtcgc gaagacggtc ttcagcggcc ccgaaggggt ggtggtgacc ggactgacca
      121 acggtggcct gtacacgtgc aaggcgctgg gcgcgaacgc cattgggacc ggtccctact
      181 ctgcgccgtc ggcgcagttc cgggtctcgg cgccgccggc cgcgccgacc ggcgtgtcgg
      241 ccgctcccgg cagtggttcg gcgatcgtgt cgttcacgcc gggtgccaac gggggtaacc
      301 ccgacgtcgc gttcgtcgtg caatgcactt cgagcaacgg cggcgtcggc ggtgggaact
      361 tcttcacggc atcgccggcc acggtgaccg gcctgacgaa cggcaagacg tacacgtgca
      421 cggtcatcgc ggcccacgca caaggcgcgt cggcaccgtc gtcggcttcg aacgcgatcg
      481 tgatcggtgt gccgagcgca ccgaccgcgg tgagcgccac gccgaggaac ggtgaggcga
      541 ccgtgtcgtg gaccgcgccg gcgaacaacg gctcggcgat caccaactac atcgtcacgc
      601 cgtacatcgg tgctgcggca cagacggcgc agaccttcaa cgcgagcgcc acgtcgcggg
      661 tcgtgaccgg cctgacgaac agcagcgtgt acacgttcaa ggttgcggcg gtcaacgcgc
      721 ggggcaccgg tgcccagtcg acggcgtcga acacggtgac ggtcggtacg ccggccgcgc
      781 cgccggcggc ctcggcggtt ccgggtgccg cgcaggcgac cgtgtcttgg accgcgccgg
      841 cgaacaacgg ctcggcgatc accggttacc aggtggtccc gatcgtcaac ggcacgccgc
      901 aggcaccacg tctgtttgcg tcgacggcga cgagtcaggt gatcaccggg ctcacgaacg
      961 ccacgacgta cacgtttgcg gtggcggcgg tgaacgcccg gggcacgggt ctggccaaga
     1021 ccacggccgc gatcacggtc ggtgcgccga ctgcaccgcc gttggtgacc gcgacagctg
     1081 gtgccggcaa ggccacggtg acgtggaccg cgcccgccag caacaacggt gcggcgatca
     1141 cgaactacat cgtgacgccg ttcatcggcg gcgtcgcgca gacaccggtg acgttcaacg
     1201 ccagccagct gacgcgacaa atcagcgggc tgacaccggg aacggcctac acgttcacgg
     1261 tgtccgcagt gaactcgcgg ggtacgggcc cggcgtcggc gccgtcgaac tcggcgactc
     1321 cgacgtagac caaccgcacc ccccgtgcga atactcgtgt ggtgccggca accaggtggt
     1381 gccggcacca cacggggtta acctgcggcc gcacgaaccg gcgtgagatt cgggcgcgat
     1441 gagcgccaaa tccacgccgg ttcgtattcc atgcgggcgc atatatctct aggctgacgc
     1501 catgagcacg atgtgggtca ggcggccgac ggccgcaccg gcgggggacg tggccgcgcc
     1561 gacgttcaaa ctggagcggc cgttggcggg tgtgacggtc gggatccgga cggacacggc
     1621 gtggcggtcg tggcggctga tcgcgcaaca gtgggacgag tggctgcggc gcgacggcgc
     1681 gcagacggtg ctcgtcgaga ccggtgggca agtcggcgcg gtcggcgccg acgaccgcaa
     1741 gcacatcgtc gagctggccc agaccgtcga ctgcgcgatc gtcggcctcg gtacgtgcgg
     1801 atcgtgcacg acgttcacgg tgaaggactc cgtgacggtc gaacggcacg agaagccggt
     1861 cgtcgccgtc gtcaccgagg agttcgagac gcacgggcac aacgtcgcga cgcacctcgg
     1921 tcaccgcgac ctcgaactgc tcgtgctgcc gtatccgctg gaagcgcggc ccgaggccga
     1981 gctgcgcgag atcgccgaca cctactaccc gcaggtgctc gacctcctcg gggtgcaacg
     2041 atgaccctca ccagcgaacg cgtcgaagtg ccgagcgacg cgcgggccca gtacgagctg
     2101 tcgttcaccg agaagtgggg cgacggcgtg ccgttgttgc cggcgaccga cgacgcgatc
     2161 gaggcgctgc tcgcggcgac gccgtacccg gccgaccacg tcgtgtgcgt gctgccgccc
     2221 cgcaacggcg tcgcgaccgc cgagctggtc gcggtcaacg cggcgatggc cggcgtcgaa
     2281 cccgatgcgt tcccgctcgt gatcgccgcg ctcgaagcgc tctccgaacc ggagtggaac
     2341 gcgttcgggc tcacgacgac gacgtcgagc gtgttcccga tgttgatcgt caacgggccg
     2401 tgccgcgacg agctcgcgat cgactcccgc gcgagttgca tgggtggcgc cgcgggccgc
     2461 ggctcgatga cgatcggccg cgcggtcgcg ctgtgcctgc gcaacatcgg cggtcagcgc
     2521 gccggcgaga cgacgaagtc ggtgttcggg cagcccgcgc ggttcggctt ctgcatcggc
     2581 gagtgggagg agcggtcgcc gtggccgagc ctcgcgcaac ggcgcgggtt cggcgccgac
     2641 gacaacgtcg tcaccgtcca cggcgggaag ggcacgttcc cgatcgcgga catccacaac
     2701 gacgacccgc gcgatctgct gtatctgatc gccaagagcc tcgcgtaccc gctcgcgaac
     2761 atgtacctcg ggcacgcggc caacggcgag gtcgtgctcg cgatcaaccc gatgtgggcc
     2821 gaacggttcg cgcagccgtt ccccggcgtc gagcagctcc agcagtacct gcgcgacaac
     2881 gcgtggcagc cgatcgacct gtggccgccg gggaaccagg agatcctgcg caagaagggc
     2941 cgggtcgacg ccgacggtcg cgtgcacctc gtcgcccgcg cggatcagat cgtgccgatc
     3001 gtgtgtggcg gcctcggcag cctccacgcc atcggcttgc cgagcttcgg cgagagccag
     3061 atgcagtccc aagccgcccg gtccgcatag tcgtggacac cgacgcgatc gccgcggccg
     3121 tcgacgagac cgggcgcttc ctgcgcgccg acggcgccga cctcgtcttg ctcgacgccg
     3181 atcccaagac ggcgcgcgtc cggctgcagc tcgtgctcga ctccgtcgag tgcgcggact
     3241 gcgtgctgcc gccggacgcg ttgcacgaga cgatcgtcga cgcgctgcaa cgccgcgtgc
     3301 ccggcgacta cgaggtcgtc gtcgacgacc cgcgccgatg accgaacagg catggagcgc
     3361 cctcgtcacc gtctaccagt cggtgctgca cgacgtcgtc ggcgcgctcg agagcgacgc
     3421 cgggatggat tcgggcgtgt tcagcgcgct ggcgtacgtc gcgcgtgagg gcaacgatgc
     3481 gctgccgctg tccgagctcc aacgcctgtt gcacccgcgc tacagccagc cgggcgtgag
     3541 ccggctcgtg caacgaatgg aagccgacgg cctcgtggtg cggcggaccg atcccgacga
     3601 caagcgggcg agcatcgtct cgctcacatc cgccggccgg acgcggtatc gcaaggcgaa
     3661 cgaggtgtac gaggccgcgg tgggggagca cttcgcgcgc cacctcacgg cgtcggaagc
     3721 acgctcgctc gttcgaattc tggactcggt cggtcgtcga ggaagatgac cggcgcctcg
     3781 aacgcggccc gccgtgacgc gcgccgcaac gcagcgagta ccggcgtgcc gacgacgaag
     3841 atcagcgcgg cgttgcccgc ggcgcgcgca acgtcgaagc cgagcgcggt cgtgaggtgg
     3901 aatgcccaga accgttgcag gttctcgacg accgacgcgc cgggcacgaa atgcagttcg
     3961 ttcgcgccgc tcgtcgtgaa cggccagaac cacatgtcca tgacgaggcc gtagatcaat
     4021 ccggagagcg cgccgtacgc ggcgagcatc gcgacctcgc ggcggccgcg catcggcggc
     4081 aggctcccgg cgaggagccc cacccacgcg gaaccgagca tctggaacgg caaccacggc
     4141 ccgacgccgc ccgtgagcaa cgcggacgcg aacatcgtcg tcgcgccctg cacgaagccg
     4201 aacccgcgcc cgaacacacg gccgcccagc acgatgagga agaacacgcc cgtgaatccg
     4261 gtgacgcccg tgaccggcgc ccgcagcagc gcaccgcacg cgacgagcac cccgagcatc
     4321 gcgatcgcct tggcatcgag gccgccctcc gacaactcgg cgagcaggat gccgaggagc
     4381 aacggcagga tgatcacgaa cagccacggc gcgtcggtgc tgtgcgcgag gttctcgtcg
     4441 ccggtcgcgt tcgtcgtcag cggccacagg aacgcggcca gcccgaacag cgacgccgcg
     4501 acgagcatcg tcgccgactt ccagcggaac cggatgggtg ccgcgatgcg cttcatgacg
     4561 cgagtgccgc tgcgacttcg gcgaccgtca gccactcgct cggcgccagg atcttcgcga
     4621 cctgcggcgc gaagaccggg gagtggcaca cgacttcgcg cgcgggtccg tcgctgacca
     4681 cgtcgccgtc ggcgagcacg acggcgcggg tcgcgacttc ggcaacgagc tcgacgtcgt
     4741 gactggcgag cacgatcgcg tgaccgtcgg ccgcgagccc gcgcagcacg gtcgcgagcc
     4801 gggccttggc cgtgtagtcg aggccgcgcg tcggttcgtc gagcaggagc acgcgcggcg
     4861 cgggtgcgag cacgaccgca agggcgagcg cgagccgttc cccttcggac aggtcgcgtg
     4921 gatggtgctc gggatcgagg ccgtcgtgga tgcggtcgag catggcgcgc gtcgtgcccg
     4981 gcgcgaggtg gtgctcgcgg tcggcgaccg tgcactcgtc ggcgatcgac cgctcgtaga
     5041 ggatcgatcc cggatcggac ggcaccagcg cgacgcggcc gacgagcgtg tgcggcgtaa
     5101 gcgtgtgcgg cgcgtcgccc gcgacccgca cggtgcccgc cgtcggcggc cgtagaccgg
     5161 cgagcaacga caggagcgtc gacttgcccg cgccgttgcg gcccatgagc gcgatgatct
     5221 cgcctgcgtg gatgcgcaag tcgaccgcgt gcagcgcttc gacgctgccg tagcgcatct
     5281 tcacgcgcga cgccgaggcc agctcctcac ccggtgccgg ccgcgcgccg atctccggca
     5341 cgcggccgtt cagccggacg cgcaagtcgt cggcggcacg ccgggcgtcg cgcaccgaca
     5401 gcggcagcgg tgaccagccc gcgagccggc cgagcgcgac gaccggcggg gtcaccggcg
     5461 cgctcgccat catcgcggcg ggcgcacccg cgtcgacgcg tccggagtcg tcgatcgtcg
     5521 cgatgcggtc ggcgtactgc acgacccgtt cgagccggtg ctcggccagc agcacggtga
     5581 ggccgaggtc gtgcacgaag cgggtcaaca tcgcaagcac gtcttcggcg gcgctcgggt
     5641 cgagcgcgga cgtgggttcg tcgagcacga gcacgcgcgg cgacgcggtc aagacgccgc
     5701 cgattgccac ccgttgcgct tcgcccgacg acagcgtgcc gagcgcgcgg ccgcgcaggt
     5761 tcgcgatgcc gagcaggtcg agcacgtcct cgacgcggcg gcgcatgacc gcgggcgcga
     5821 caccgaggtt ctccatcaca tacgccagct cgtcctcgac cgtgtcggcg acgaagccgg
     5881 ccgcgggatt ctggcccacg acaccgacga cgtcggcgag ctcgcgcggc ggggcatcgg
     5941 ccgtcgacgt gccgttgacg gagacccgtc cggcgagggt gccaccgctg aagtggggca
     6001 ccagcccgtt gaccgcgcgc agcaacgtcg acttgccgga gccggtctcc ccgacaacga
     6061 ggcagaactc cccctcgggc acgagcagat cgacaccgcg gatggcggcg cgcgcggcat
     6121 cgggataggt gaacgtgacg gcgtcgaacc ggatcatgcc ggctccgtcg cgagcgggat
     6181 cgcgggcgtg cacacggccg gcagcgcggc gatcagcacg ccgagcgtcg gcaggagcgg
     6241 cagcgtcggc gcgacgagcg ggctgtacgc ggggc
//
LOCUS       DASUGA010000521         2148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1578157, whole genome shotgun sequence.
ACCESSION   DASUGA010000521 DASUGA010000000
VERSION     DASUGA010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578157"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VFX21_10300"
     CDS             <1..290
                     /locus_tag="VFX21_10300"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5096398.1"
                     /translation="ISGRSAYVGGSCYAATKHAVMGFSESLMLEVRDKGVKVSVVNPG
                     SVATTFSHREGDTSWMLTADDVADAVMSALDTPPRVLVHRIEVRAAIPKKG"
     gene            complement(291..>2148)
                     /locus_tag="VFX21_10305"
     CDS             complement(291..>2148)
                     /locus_tag="VFX21_10305"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX5096399.1"
                     /translation="QYVPGRSLERVVRDHGSLTLTSVGAIVYHVGSALDYGHRRGVVH
                     RDVKPANILLDADGDPIVTDFGIAKVAEAPGYTRVGTVLGTPTYMSPEQCMALDVGAA
                     SDQYSLGIVAYQMLAGHTPFAGDPMAIMRAHISDAPPPLRSARVDVPPHVEHAIMRMM
                     AKRPEDRFPDLAAAVEAFNAQPLGSLGPLRAEMSALAAAADAEGSLAEIVRPRPAAAP
                     SPVPPRPSVPPSAVSSGKTPRVAVASIDVGQPSSEIEVGDCVVLHVTPRTEGGAAIEA
                     ARLKWESSNTRVAEVDAAGVVTAKDVGTATITVSAGEVKNTVDIVVLAAEVATIEVDV
                     PAEVRAGTKTTLVARALDRHGGRIDAIVRWTSRNPGVASVTDDGTLSAKRRGAAVVVA
                     ECSGSARAVTITVTPPPVVAVVIDGMPPAAVVGTTTSLRAIARTAARAGDGDPERTFE
                     WRSSDPAVATVAADGTVTARRPGRAMISATCDGVKGQAEVTVVTVRAHSVVVSSPPSR
                     LRLGEKATLSAKVYDAQGNVVDRAVTWRSSDPGVVLVDAAGRIVAQAEGWAIVTAQAD
                     GVESPIEIVVRQQVVPVSVAGQRESRRLALRWWILLAVIAAAVAAGWRFLVR"
BASE COUNT          333 a          735 c          729 g          351 t
ORIGIN      
        1 ccatttccgg acgaagcgcg tacgtcggcg gctcctgcta cgctgcgacc aaacacgccg
       61 tcatgggctt cagcgagtcg ctgatgctcg aggtccgcga caagggagtg aaggtgagcg
      121 tcgtgaaccc cgggtctgtc gcgacgacct tttcgcaccg tgaaggggac acgagctgga
      181 tgctcacggc cgatgatgtc gccgacgccg tcatgagtgc gctcgacacg ccgccgcggg
      241 tgctcgtgca ccggatcgag gtgcgtgccg cgattccgaa aaagggctag tcagcgtacg
      301 aggaagcgcc acccggccgc gacggcggcc gcgatgacgg caagcaggat ccaccaccgc
      361 agagcgagcc gccgcgactc ccgctgccct gccacggaca cgggaaccac ctgctgccta
      421 acgacgatct cgatcggtga ttcgacgccg tcggcctgcg cggtgacgat ggcccagccc
      481 tcggcttgcg cgacgatccg gcctgcggcg tccaccagga cgacgccggg gtcgctcgac
      541 cgccacgtca cagcgcggtc gaccacgttg ccttgcgcgt cgtacacctt cgccgagagc
      601 gtggccttct ctccgaggcg aagccgcgac ggtggtgagc tcacgacaac ggagtgcgcg
      661 cgcaccgtca caacggtgac ttccgcctgt cctttgacgc catcacacgt cgcgctgatc
      721 atggcgcgcc caggtcttcg cgcggtgacg gttccgtcag cggcgacggt tgcgacagcc
      781 ggatcgctcg atctccactc gaacgttcgc tccggatcgc cgtcgcccgc gcgtgccgcg
      841 gtcctggcga tggcacggag tgacgtcgtc gtgcccacca cggctgcggg tggcatgccg
      901 tcgatgacga cggccactac cggcggcggc gttaccgtga tggtcactgc tcgcgccgag
      961 ccgctgcatt ccgcaacgac gactgccgcg ccacgtcgct ttgccgagag cgtgccgtcg
     1021 tccgtcaccg acgcgacgcc cgggttgcgc gaggtccatc ggacgatggc atcgatccgt
     1081 ccgccgtgtc ggtcgagcgc gcgagccacg agagtggtct tggtgcctgc gcgcacttcg
     1141 gccggaacgt cgacctcgat ggtcgcgact tcggcagcga ggacgacgat atccaccgtg
     1201 ttcttcactt caccggcgga taccgtgatc gtggcggtgc caacgtcttt ggcagtgacc
     1261 acgccggctg catcaacctc ggcgacgcgc gtattcgacg actcccactt gagacgtgcc
     1321 gcctcgattg cggccccgcc ttccgtcctc ggtgttacgt gcagcacgac gcagtcacca
     1381 acctcgatct cgctggacgg ttgaccgacg tcaatcgagg cgacggctac gcgcggtgtt
     1441 tttccactgg agaccgctga cggcggtacc gaggggcgcg gcggtaccgg actcggcgcc
     1501 gcggccggac ggggcctaac gatttccgcg aggctgccct ccgcgtcggc agccgccgcg
     1561 agcgccgaca tttcggcacg gagcggccca agcgagccga gtggctgggc gttgaacgcc
     1621 tccacggcag ccgcgaggtc gggaaaccga tcctcggggc gcttggccat catgcgcatg
     1681 atcgcatgct cgacgtgggg cggcacgtcg actcgcgcgg aacgaagcgg aggcggcgcg
     1741 tcgcttatgt gcgcgcgcat gatcgccatc gggtcaccgg cgaacggcgt gtgtccggcg
     1801 agcatctggt acgcgacgat tccgagcgag tactggtcgc tcgccgcacc cacgtcgagc
     1861 gccatgcact gctccgggct catgtaggtt ggcgtgccga ggacggtgcc gacgcgcgtg
     1921 tatcccggtg cttcggcaac cttcgcgatt ccgaagtccg tcacgatcgg gtcgccgtcg
     1981 gcgtccagca gaatgttcgc gggttttacg tcgcgatgaa cgacgcctcg gcggtgaccg
     2041 tagtcgagtg ccgagccgac atggtacacg atcgcgccga ccgaggtgag cgtcagactt
     2101 ccgtgatcgc ggacgacgcg ctcgagcgac cggccgggca cgtactgc
//
LOCUS       DASUGA010000522         2941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_918069, whole genome shotgun sequence.
ACCESSION   DASUGA010000522 DASUGA010000000
VERSION     DASUGA010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918069"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFX21_10310"
     CDS             <1..573
                     /locus_tag="VFX21_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HEX5096400.1"
                     /translation="PLSALHRAETFTPDELPARYAGFSTCFRREAGTYGKDTRGIFRV
                     HQFDKVEMFSFCPPDQSWEELDRILAVEEDIVGGLGLPYRVVNIAAGDLGPAAAKKFD
                     IEVWLPSEGQYREATSCSNYTDYSARRMGTRVKGTNGSELIHTLNGTGCAVSRTLVYL
                     FEHYQDADGGFTVPEVLRPFTGFDRVAPRK"
     gene            670..755
                     /locus_tag="VFX21_10315"
     tRNA            670..755
                     /locus_tag="VFX21_10315"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:704..706,aa:Ser,seq:tga)
     gene            846..1223
                     /locus_tag="VFX21_10320"
     CDS             846..1223
                     /locus_tag="VFX21_10320"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5096401.1"
                     /translation="MKLPAQTMLHAQDLDATIAWYRDVLGFSLSATWPDTDPTWCAID
                     YGPATLMFTNNPADGSHESHMSGELCFYPDDIDRYYDEVTARGAQAIQPPADQVFGMR
                     TFLLTDPNGYTLMFAQGLDELDR"
     gene            1237..1602
                     /locus_tag="VFX21_10325"
     CDS             1237..1602
                     /locus_tag="VFX21_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014446110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5096402.1"
                     /translation="MTLRVGNITFDCDDALKQARFWSAAIGRPVDPGGSQGYASIGGT
                     DPAREQPAWYFEKVPEAKHAKNRMHLDFVDPDPAAVDRLVSLGATVVAVHEMSGHRWT
                     VMNDPEGNEFCVAAKSYGC"
     gene            complement(1641..2093)
                     /locus_tag="VFX21_10330"
     CDS             complement(1641..2093)
                     /locus_tag="VFX21_10330"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5096403.1"
                     /translation="MSYQRAVAYLTDPASRLLVFDHVRSADAGTQVPAGGIHAGEAPE
                     AAAMRELVEESGISAARMVRKLGECWWNADGRPVPTGYEEQMHHVFHLHLDAPTAAAE
                     WEWDERDGDHLSGHRFAFRWIDLAAAETQLFPEQAMWIALLRLSIIRM"
     gene            complement(2090..2497)
                     /locus_tag="VFX21_10335"
     CDS             complement(2090..2497)
                     /locus_tag="VFX21_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020113210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX5096404.1"
                     /translation="MPDRPEVPNQVVAKLAKLCLALPEVFQEDAWVGTRWKVRKETFA
                     HVLMIADGWPPAYVKAAGTEGPACVLTFRAPIEELDAMRGAPFFKPVWFADVVGLFLD
                     DGTDWTEVRELVTDSYCILAPKKLAALVDRPDG"
     gene            2516..>2941
                     /locus_tag="VFX21_10340"
     CDS             2516..>2941
                     /locus_tag="VFX21_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5096405.1"
                     /translation="MPEYEFEFEEMQNARFREVDLSGARMHSMNLSNLKVTDAFIFNV
                     ELSGIVGNLKVNDVDVTGYVEAELNKRYPERLLLAPTDPAGFRTGWDAVTGLWAKTME
                     RARALPDATLDESVDGEYTFLETLRHLVFAIDRWITEPVL"
BASE COUNT          486 a          997 c          975 g          483 t
ORIGIN      
        1 ccgttgtcgg cgctgcaccg ggccgagacc ttcacccccg acgaactgcc cgcgcgctac
       61 gccggtttct cgacctgctt ccggcgcgag gcgggcacct acggcaagga cacgcggggt
      121 atcttccgcg tgcaccagtt cgacaaggtc gagatgttct cgttctgccc gccggatcag
      181 tcgtgggaag agctcgaccg gatcctcgcc gtcgaagaag acatcgtcgg cggcctcggc
      241 ctcccgtacc gcgtggtcaa catcgcggcc ggcgatctcg gtccggccgc ggcgaagaag
      301 ttcgacatcg aagtgtggct gccgtcggag ggtcaatacc gcgaagcgac gtcgtgctcg
      361 aactacaccg actactcggc gcgccggatg ggcacccgcg tgaagggcac gaacggcagc
      421 gagctgatcc acacgctcaa cggaacggga tgcgccgtga gccgcacgct cgtgtacctg
      481 ttcgagcact accaggatgc cgacggcggc ttcaccgtgc ccgaggtgct ccgcccgttc
      541 accggcttcg accgggtcgc gccgcgcaag taagtcgacc gcgccgttct cggtcaatct
      601 gacccgctct cagcgggcca acgttcccca gaatgcgcgc cccacgcgcc tcggtatcgt
      661 ggtcggtctg gagggatgcc ggagcggccg aacggggcgg tcttgaaaac cgttgtgcgc
      721 aagcaccctg ggttcgaatc ccagtccctc cgccacgtgc gccgcggttc tcggtcagtc
      781 tggcccgctc tgatcgggcc gggattccaa tgaacgacga gcggcggtac gttcgcgacg
      841 tggcgatgaa gctgccggcg cagacgatgc tgcacgcgca ggatctcgac gcgacgatcg
      901 cctggtaccg cgacgtgctc ggcttctcgc tctcggcgac gtggcccgac accgacccga
      961 cgtggtgcgc gatcgactac gggccggcga cgttgatgtt caccaacaat cccgccgacg
     1021 ggtcgcacga gtcgcacatg tcgggtgagc tctgcttcta ccccgacgac atcgaccgct
     1081 actacgacga ggtcaccgcg cgcggcgcgc aggcgatcca accgcccgcg gaccaagtct
     1141 tcggcatgcg gacgttcctg ctgaccgacc ccaacggtta cacgctgatg ttcgcccaag
     1201 ggctcgacga gctcgaccgc tgaatgaggg ggttccatga cgttgcgtgt cggcaacatc
     1261 acctttgatt gcgacgacgc gttgaagcag gcgcggttct ggtcggccgc gatcggtcga
     1321 ccggtggatc ccggcgggag ccagggttat gcgtcaatcg gtggcaccga cccggcgcgt
     1381 gagcagccag cgtggtactt cgagaaggtt cccgaggcga agcacgcgaa gaatcgcatg
     1441 cacttggact tcgtcgatcc ggaccccgca gccgtcgatc gactcgtgtc gctcggcgcc
     1501 accgtcgtgg cggtgcacga gatgtcgggg catcggtgga ccgtcatgaa cgaccccgag
     1561 ggcaacgagt tctgcgtggc cgccaagtcg tacggctgct gaagccggca cgcaccggcg
     1621 agggctatcg cacatgccgg ttacatccga atgatcgaga gccgcagcag cgcgatccac
     1681 attgcttgct cggggaacag ctgcgtttcg gccgcggcga ggtcgatcca gcggaacgca
     1741 aagcggtggc ccgacaggtg gtcgccgtcg cgctcgtccc attcccactc ggccgcggca
     1801 gtcggcgcgt cgaggtgcag atggaacacg tgatgcatct gctcctcgta gcccgtcggc
     1861 accggtctgc cgtcggcgtt ccaccagcat tcgccgagct tgcgcaccat ccgcgccgcg
     1921 ctgatgccgg actcttcgac gagctcgcgc atcgccgcgg cctccggtgc ttcgccggcg
     1981 tggatacctc cggcgggcac ttgcgtcccc gcgtcggcac tgcggacgtg atcgaagacc
     2041 agcagccgcg acgccggatc cgtgaggtac gcgaccgcgc gctggtagct catccgtcgg
     2101 gccggtcgac gagcgcggcg agcttcttcg gggcgaggat gcaatagctg tcggtgacga
     2161 gctcgcgtac ctcggtccag tcggtgccgt cgtcgaggaa caggccgacg acgtccgcga
     2221 accacaccgg cttgaagaac ggcgcgccgc gcatcgcgtc gagctcctcg atcggcgcgc
     2281 gaaacgtcag cacgcatgcc gggccttcgg tccccgcggc tttgacgtag gcgggcggcc
     2341 agccgtcggc gatcatgagc acgtgcgcga acgtctcctt gcgcaccttc caccgggtgc
     2401 cgacccacgc gtcttcctgg aacacctcgg ggagcgcgag gcagagcttc gccaacttcg
     2461 cgacgacctg attcggcact tccggccggt cgggcatggc gcgatgattg cacgcgtgcc
     2521 ggagtacgag ttcgagttcg aagagatgca gaacgcacgt ttccgtgagg tcgatctctc
     2581 gggcgcgcgg atgcacagca tgaacctctc gaacctgaag gtcaccgacg ccttcatctt
     2641 caacgtcgag ctgtcgggca tcgtcggcaa cctgaaggtg aacgacgtcg acgtcacggg
     2701 atacgtcgaa gcggagctca acaagcgcta ccccgagcgc ctgctgctcg cgccgacgga
     2761 tcccgcgggc ttccgcacag ggtgggacgc ggtcaccggt ctctgggcaa agacgatgga
     2821 gcgtgcccgc gcgctgccgg acgcgacgct cgacgagtcg gtcgacggtg agtacacgtt
     2881 cctcgagacg cttcggcacc tcgtgttcgc catcgaccgc tggatcaccg agccggtcct
     2941 c
//
LOCUS       DASUGA010000523         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_861884, whole genome shotgun sequence.
ACCESSION   DASUGA010000523 DASUGA010000000
VERSION     DASUGA010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861884"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VFX21_10345"
     CDS             complement(<1..257)
                     /locus_tag="VFX21_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015278278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5096406.1"
                     /translation="MKALVTGGAGFIGSHLVDALLGAGHEVHVLDDLFTGFRENVPAA
                     ATLHVGSVADEDLVRTAVAGCEVVFHPAAHKAVLRSVEQPLP"
     gene            complement(254..775)
                     /locus_tag="VFX21_10350"
     CDS             complement(254..775)
                     /locus_tag="VFX21_10350"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5096407.1"
                     /translation="MQRETCAVAFVDVVGFTAGTARYGDGYAASIVDALEDAVRAALP
                     PGARLVKLLGDGAMCVFPDPTDAVRAMTDVIAQLAAARTAIEARAAVHFGLVTVRGDD
                     VIGHVVNVTARLTARARPGEVLCSDAARAAVHDDALVFADRGVLDLRGIGGGLPVFGA
                     HHRPIGCDVAFSG"
     gene            complement(778..993)
                     /locus_tag="VFX21_10355"
     CDS             complement(778..993)
                     /locus_tag="VFX21_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096408.1"
                     /translation="MKRHRFDPLAFSAGVVFLVLAVAFGLDALDTWSTNAVWVPPVVF
                     IALGLGSLFTTLARNHQRAEQTADESA"
     gene            complement(993..>2130)
                     /locus_tag="VFX21_10360"
     CDS             complement(993..>2130)
                     /locus_tag="VFX21_10360"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LiaF domain-containing protein"
                     /protein_id="HEX5096409.1"
                     /translation="AAFLGIGIPAYIVACIVIPLESGADGEPVPERETSTVIGLALLA
                     LGGFWLATQLLPHRLHLVDVIWPIALIGGGIAIIVLRTNAREDEPEPPPFAPWEPTAP
                     SAPTMTIADIADTDIADAPADEAPEEVEEAEPDEPTASSATSTSSAWQQNRAWIPPVP
                     KPPRPPRPPKPRGFLGPIVFSLLCIAGGIVALLDTTNTVDVNWEGAIAIGLGVVGAAL
                     VLSTWYGRARGLIGLGALMTAVLTLTSFVNVPLEGGIGDRDYRPVNSAELESVYRLAI
                     GQMTLDLRDMPFEDGVTEIEASVAMGELRVEVPSDVTVEVRAHVQAGDIEVFGDDENG
                     WDVDVNRRVGTGEQVVRLDLKVGTGHIWVGRYSADGIETIPGGN"
BASE COUNT          336 a          737 c          738 g          319 t
ORIGIN      
        1 ggcaggggct gttcgaccga gcgcagcact gctttgtgcg cggccgggtg gaacacgacc
       61 tcgcagccgg cgaccgcggt ccggaccagg tcttcgtcgg ccaccgatcc gacgtgcagc
      121 gtcgcggcgg ccggcacgtt ctcgcggaac cccgtgaaca ggtcgtcgag cacgtgcacc
      181 tcgtggcccg cgccgaggag tgcgtcgacg agatgggacc cgatgaaccc cgcgccgccg
      241 gtaacgagcg ccttcatccg gagaaggcta cgtcacatcc aattggccga tggtgcgcgc
      301 cgaagacggg caatccgccg ccgatgccgc gcaagtcgag cacaccgcga tccgcgaaca
      361 cgagggcgtc gtcgtgcacc gccgcgcgtg cggcgtcact gcacagcacc tcgccgggcc
      421 gggctcgcgc ggtcagacgc gccgtcacgt tgacgacgtg accgatgaca tcgtcgccgc
      481 gcaccgtgac gaggccgaag tgcaccgcgg cccgagcctc gatggccgtg cgcgcggcgg
      541 cgagctgcgc gatcacatcc gtcatcgcac gcactgcatc tgtcgggtcg gggaacacgc
      601 acatggcgcc gtcgccgagc agcttcacca aacgggcgcc cggcgggagc gcggcgcgca
      661 ctgcgtcctc gagcgcgtcg acgatgctcg ccgcgtaccc gtcgccgtag cgtgccgtcc
      721 cggcggtgaa gccgacgacg tccacgaagg caaccgcgca cgtttcgcgc tgcacggtca
      781 ggcgctctcg tccgcggtct gttccgcacg ctggtgattg cgggccaggg tcgtgaacaa
      841 gctgccgagc ccgagcgcga tgaacacgac gggcggcacc cacaccgcgt tcgtcgacca
      901 ggtgtcgagc gcgtcgagac cgaacgcgac ggcgagcacc aagaacacga caccggccga
      961 gaacgcaagg gggtcgaagc gatggcgttt catcagttgc ctccgggaat cgtctcgatg
     1021 ccgtcggcgg agtagcgacc gacccagatg tgaccggtgc cgaccttcag gtcgaggcgc
     1081 accacttgtt cgccggttcc cacacgccgg ttcacgtcga catcccagcc gttctcgtcg
     1141 tcgccgaaca cctcgatgtc gcccgcttgc acatgggcgc gcacttcgac cgtgacgtcg
     1201 ctcggtactt cgacccgcag ttcgcccatc gcgacgctcg cctcgatctc cgtcaccccg
     1261 tcctcgaacg gcatgtcgcg cagatcgagg gtcatctgac cgattgcgag gcggtatacg
     1321 gattcgagtt cggccgagtt caccggtcgg tagtcgcgat cgccgatacc accctcgagg
     1381 gggacgttca caaagctcgt gagcgtcagc accgcggtca tgagcgcacc gagcccgatg
     1441 agcccgcggg cgcggccgta ccacgtgctc aacacgagcg cggcgccgac gacgccgagt
     1501 ccgatcgcga tcgcgccttc ccagttgacg tcgacggtgt tcgtagtgtc gagcaacgcg
     1561 acgatgccgc ctgcgatgca caggaggctg aagacgatcg gtcccagaaa accgcgcggt
     1621 ttcggtggac gcggcggacg cggcggcttg ggcaccggtg ggatccacgc gcggttctgt
     1681 tgccacgcgc tcgacgtcga ggtcgcgctc gacgcggtcg gctcgtcggg ctccgcttct
     1741 tcgacctcct ccggcgcttc gtcggccggc gcgtccgcga tgtcggtgtc ggcaatgtcg
     1801 gcgatcgtca tcgtcggcgc gctcggcgcg gtcggttccc acggcgcgaa cggcgggggc
     1861 tcgggttcgt cttcgcgtgc gttcgtccgc aggacgatga tcgcgatgcc gccgccgatc
     1921 agcgcgatgg gccagatgac gtcgacgaga tgcaggcggt ggggcagcag ttgggtcgcg
     1981 agccagaacc cgccgagcgc gagcagcgcg agtccgatga cggtgctcgt ctcgcgttcg
     2041 ggcaccggtt cgccgtccgc gcccgattcg agcgggatga cgatgcacgc aacgatgtag
     2101 gccgggatac cgatgccgag gaacgcggcg
//
LOCUS       DASUGA010000524         2550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_288330, whole genome shotgun sequence.
ACCESSION   DASUGA010000524 DASUGA010000000
VERSION     DASUGA010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288330"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VFX21_10365"
     CDS             <1..682
                     /locus_tag="VFX21_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX5096410.1"
                     /translation="DAYRVQQLVSHLREPAPPRVVGRKVGLTSPVVQRQMGVDEPDFG
                     VLTADMAFADAQPMPTELLMQPMIEGEIAFVLGADLDRMPVRAADVMRATEFVVAAIE
                     VVDSRIANWDINIFDTVSDNASSGVFVLSGTPRLLSALDDLRDVAMTLECDGDVVSSG
                     TGAACLGHPVNAVTWLANTVATRGAPLLAGEVVLSGALGPLVPVRPGAVYEARLAGLG
                     SVRANFGS"
     gene            758..1660
                     /locus_tag="VFX21_10370"
     CDS             758..1660
                     /locus_tag="VFX21_10370"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HEX5096411.1"
                     /translation="MQTTPTAAIVGSGNIGTDLMYKLLRSRRLELRYVVGIEPDSDGL
                     ARARREGLETSAHGAPWLLRHAEPPDLVFEATSAHVHEQNAAAYRDAGIRAIDLTPAA
                     VGPFVIPPVNLAEHVDEANVNLVTCGGQATVPLVHAVSRVTPVAYAEIVATVSSRSAG
                     PGTRNNIDEFTRTTSAAVVSLGGSSQGKAIIILNPAKPPLIMRDTVYCSLEPGADVDA
                     VESSINDMVARVQDYVPGYRLLQPPTFDEATDGERGRVAMFVEVEGAGDYLPSYAGNL
                     DIMTAAAARVGDEIARRMKEGSPT"
     gene            1660..>2550
                     /gene="dmpG"
                     /locus_tag="VFX21_10375"
     CDS             1660..>2550
                     /gene="dmpG"
                     /locus_tag="VFX21_10375"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016883402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008701 - 4-hydroxy-2-oxovalerate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HEX5096412.1"
                     /translation="MPYSDTLDVRVTDSALRDGSHAKQHQFTADDVRRVVSALDHAGV
                     PVIEVTHGDGLAGSSYNYGFSRTPEQVLLRSAAEAATNAKIAALMLPGIATTDDIAAA
                     QDNGVSIIRIATHCTEADVSVQHFGVSRRLGLETVGFLMMAHTVPPEKLVEQGRIMVD
                     AGCQCVYVVDSAGALVLDEVTERVVALREELDASIHVGFHGHQNLSLGVANTVLAIRA
                     GATQVDSCTRAFGAGSGNTPTEALAAVLTKLGITTGIDPLAMFDAAEEVVRPVMDDEC
                     LLDRLGLIMGYTGVYSSFLKH"
BASE COUNT          413 a          875 c          855 g          407 t
ORIGIN      
        1 cgacgcgtac cgggtccagc agttggtctc acacctgagg gagccggccc cgccacgcgt
       61 cgtcggccgc aaggtcggcc tgacgtcgcc ggtcgtgcaa cggcagatgg gcgtcgacga
      121 acccgacttc ggcgtgctca cggccgacat ggcgttcgcc gatgcacagc cgatgccgac
      181 cgaactgctc atgcaaccca tgatcgaagg cgagatcgcg ttcgtcctcg gtgccgatct
      241 cgaccgcatg ccggtgcgag ccgccgacgt gatgcgggcg acggaattcg tcgtcgccgc
      301 gatcgaggtc gtcgacagtc gcatcgcgaa ctgggatatc aacatcttcg acactgtcag
      361 cgacaacgcg tcgtccggtg tgttcgttct gtcggggacg ccgcggctcc tgtcggcgct
      421 cgacgacctc cgcgacgtgg cgatgaccct cgagtgcgac ggcgacgtcg tcagctcggg
      481 tacgggcgct gcgtgcctgg gccatcccgt gaacgccgtg acatggctcg cgaacacggt
      541 cgccacacgt ggtgcaccct tgctcgcggg cgaggtggtg ctctcgggtg ctctcggccc
      601 gttggtaccg gtgcgccccg gcgccgtgta cgaggcgcgt ctcgcggggc tcggctcggt
      661 gcgcgcgaac ttcgggtcgt agccggcgcg acggggccgt cccgccgagt ggcacgacct
      721 gtgcgtccgg cgcgatgaga ccctgcatga tcgccccgtg caaacgactc ccacggcggc
      781 gatcgtcgga tcgggcaaca tcggcaccga cctcatgtac aagttgctgc gctcgcgccg
      841 gctcgagttg cgctacgtcg tcggcatcga gccggacagt gacggcctcg cacgggcgcg
      901 ccgggagggc ctcgagacgt ccgcgcacgg tgcgccgtgg ctcctgcgac acgccgaacc
      961 accggacctt gttttcgagg cgacgtcggc ccacgtacac gagcagaacg ccgcggccta
     1021 ccgggacgct ggaatccgcg cgatcgacct cacccccgcg gccgtcggcc cattcgtgat
     1081 cccaccggtg aatctcgcag aacacgtcga tgaagcgaac gtgaacctcg tgacctgcgg
     1141 cgggcaggcg accgtgccgc tcgtgcacgc cgtgtcgcgc gtgacaccgg tcgcgtacgc
     1201 ggagatcgtc gcgacggtct cgtcacggtc ggcgggcccg gggacgagga acaacatcga
     1261 cgagttcacg cgtacgacgt ccgcggcggt cgtctcgctc ggcgggagct cgcagggcaa
     1321 ggcgatcatc attctgaacc cggcgaaacc cccgctcatc atgcgagaca ccgtctactg
     1381 ttcgctcgag ccgggtgccg acgtggacgc cgtcgagtcg tcgatcaacg acatggtcgc
     1441 gcgtgtgcag gattacgttc cgggctatcg cctcttgcag ccgccgacgt tcgacgaagc
     1501 caccgacggc gagcggggac gagtcgcgat gttcgtcgaa gtcgaaggcg ccggcgacta
     1561 cctcccgagc tatgccggca acctcgacat catgaccgcc gcggcggcgc gggtgggaga
     1621 cgagatcgcg cgccgcatga aggaaggatc acccacctga tgccctactc cgacacactc
     1681 gacgttcgcg tcaccgactc cgcgctccgt gacggttctc acgcgaagca gcaccagttc
     1741 acggccgatg acgtcaggcg ggtcgtcagc gcgctggacc acgccggcgt tcccgtgatc
     1801 gaggtcacac acggagacgg cctcgccgga tcgtcgtaca actacggctt ctcgcgcaca
     1861 cccgagcagg tactgctgcg cagcgcggcc gaagcggcga ccaacgcgaa gattgcggcg
     1921 ctgatgctgc cgggcatcgc gacgacggac gacatcgcgg ccgcgcagga caacggcgtg
     1981 tcgatcatcc gtatcgcgac gcactgcacc gaagccgacg tctcggtgca acacttcggt
     2041 gtctcacgcc ggctgggcct cgagacggtc ggcttcctga tgatggcgca tacggtgcca
     2101 cccgagaagc tggtcgagca gggtcgcatc atggtcgatg ccggctgcca atgcgtgtac
     2161 gtcgtcgact cggccggcgc gctcgtactc gacgaggtca ctgagcgcgt tgtcgcgctg
     2221 cgcgaggaac tcgacgcgtc gatccacgtc ggtttccacg gccaccagaa cctctcactc
     2281 ggcgtggcga acactgtgct cgcaatccgg gccggtgcga cccaagtgga ctcgtgcaca
     2341 cgcgcgttcg gcgccggctc cgggaacacg ccaaccgagg cgttggccgc ggtgctcacc
     2401 aagctgggca ttacgaccgg gatcgatccc cttgcgatgt tcgacgccgc ggaggaggtc
     2461 gtccgcccgg tgatggacga cgagtgcctg ctcgatcgac tcggcctcat catgggctac
     2521 accggcgtgt attcgagctt cctcaagcat
//
LOCUS       DASUGA010000525         4782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_97602, whole genome shotgun sequence.
ACCESSION   DASUGA010000525 DASUGA010000000
VERSION     DASUGA010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97602"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VFX21_10380"
     CDS             <1..878
                     /locus_tag="VFX21_10380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX5096413.1"
                     /translation="FPLLLAGEHTERLELTTAVAIAFARNPMTLAGTAYDLQLASKGR
                     FNLGLGTQIKPHIEKRFSMPWSHPADRMRELVLAVRAIWASWHEGAKLDFRGDFYTHT
                     LMTPFFDPGPNPYGLPRIFLGGVGPRMTEVAGEVADGFMIHPFSTRAFIEQTTMPALQ
                     RGLDRAGRSRADIQIAFPIMVVAADTDDELARGVNGMRARLAFYGSTPAYKRVLDTHG
                     WGDLQPELNTLSKRGGWPEMSALITDEMVDELCIVGKPAEIPEKLQARYGDIVDRASF
                     DTTPPDHRLIRYFKP"
     gene            complement(880..2334)
                     /gene="gatB"
                     /locus_tag="VFX21_10385"
     CDS             complement(880..2334)
                     /gene="gatB"
                     /locus_tag="VFX21_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010277366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX5096414.1"
                     /translation="MTTWETVVGLEVHCELSTASKLFCGCPNEFGTEPNTNVCPVCLG
                     LPGSLPVLNEKAVEYALRVAEALNLKVPAQSVFARKNYFYPDMPKDYQTSQYDEPITI
                     DGFLDVDDTRIGITRAHLEEDTGKSLHVGGSGRIQDADHSLVDYNRAGVPLLEIVSEP
                     DIRSAEQAKRYVEELRATLLAIGVSDVKMEEGSLRIDANVSVRPEGSTEFGTRCEIKN
                     LNSLRSLTKAIEHEAKRQVTAREFGDDIVQETRHWDEARGRTVSGRSKEEAHDYRYFP
                     EPDLVPVAPTDEMRTRVHETMPELPAAHRARLVEEWGIKPDDARVLVSTPGLSDYAAK
                     AVAALTAGTPKDVVNWAVQEVLAYLNKTNLAPAVLSPEMLGELVSLVADGKISRNQGK
                     DVLDESLREDKWPRDIVEARGLGQVTDTGELAAVVDAVLVRNADAVAEFRAADDKGKK
                     KKRGFLMGELMKEMKGQGDPVTLNQLLDERLNAS"
     gene            2354..3274
                     /locus_tag="VFX21_10390"
     CDS             2354..3274
                     /locus_tag="VFX21_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5096415.1"
                     /translation="MAAAPDPNAFATHRADVRNGVTIAYVREGVGGTPLLLLHGYPET
                     KRIWWRNIGPLAAAGFEVIAPDYRGHGDSSLAPDNFYDIAAFAIDCYTLVHDVLGHES
                     CAVAGGDVGGIVLFDIGLRYPGFVTRQVLFNTLPPPLYDVYQAAGLGPDDDRATRATA
                     DYFRRQSTEPDVLLAELDTPARRQAWVAPMYGHRLWGTPHAFTDEEVAFHSEPFADAA
                     KLRASWGVYEQAAGQRPMEDVPKLFEPTPVPTMVLYGTEDHVVPKSFPWKAAAACLDC
                     TGPLFVPNAGHFLQWESADTFNKITAAFCR"
     gene            complement(3278..4150)
                     /locus_tag="VFX21_10395"
     CDS             complement(3278..4150)
                     /locus_tag="VFX21_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5096416.1"
                     /translation="MRVGAHLPLMDFGGNPYSREHLVAYARAARDLGFDAVSANDHMV
                     FSVPWLDGLAALGAVVEHTGTMNLVTTVALSVVRGPVPLAKSLAAIDRLSGGRVIAGV
                     GPGSSADDYATVGIDFGERWPRFEASVLELRAQWTRLAPPPVQPDGPPVWIGSWGSAA
                     GLRRVARLADGWLASAYNTTPALFADAWQQLQSLLGARADGFPNALATMWCYITDDDA
                     EAERVLVERVVPTVHRPEAVLRERLPIGSPVRFAEKLRAFADAGVQQVFIWPVADEVE
                     QLHRFATQVRPLLA"
     gene            complement(4185..>4782)
                     /locus_tag="VFX21_10400"
     CDS             complement(4185..>4782)
                     /locus_tag="VFX21_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015142700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX5096417.1"
                     /translation="VDGAAQALESAGAKVDRVSIPSATYGLSAYYLIAPAEASSNLAR
                     YDGVRYGLRVDGNDVAEMNAKTRDAGFGREVKRRIMLGTYALSAGYYDAYYGQAQRVR
                     TLILRDFARAYETFDVLLSPTSPTVAFEAGAKTADPLAMYLCDVCTIPTNLAGHPGIS
                     VPFGTGAHGLPVGVQVLADALGEEKMFRAARALEDVRR"
BASE COUNT          718 a         1713 c         1600 g          751 t
ORIGIN      
        1 tttttccact gctgctcgcg ggcgagcaca ccgagcggct ggagctcacc accgcggtcg
       61 cgatcgcctt cgcccgcaac ccgatgacac tggccggcac cgcgtacgac ctccagctcg
      121 cgtcgaaggg tcggttcaac ctcgggctcg gcacgcagat caagccgcac atcgagaaac
      181 gcttctcgat gccgtggtca cacccggccg accgcatgcg cgagctggtg ctcgcggtcc
      241 gtgccatctg ggcgagctgg cacgagggcg cgaagctcga cttccgcggc gacttctaca
      301 cgcacacgct gatgacgccg ttcttcgacc cggggccgaa tccctatggt ctgccgcgca
      361 tctttctcgg cggcgtcggt ccgcgcatga ccgaggttgc gggcgaagtc gccgacggct
      421 tcatgatcca cccgttctcg acacgcgcgt tcatcgagca gacgacgatg ccggcactgc
      481 aacgcggcct cgaccgcgcg ggccggtcac gcgccgacat ccagatcgcg ttcccgatca
      541 tggtggtcgc cgccgacacg gacgacgagc tcgctcgcgg cgtcaacggg atgcgggcgc
      601 ggctcgcgtt ctacggctcg acgcccgcct acaagcgcgt gctcgacacg cacgggtggg
      661 gcgatctgca accggagctg aacacgctgt cgaagcgcgg tgggtggccc gagatgagcg
      721 cgctgatcac cgacgagatg gtcgacgagc tgtgcatcgt cggcaagccc gcggagatcc
      781 cggagaagtt gcaggcccgc tacggcgaca tcgtcgaccg cgcgtcgttc gacacgaccc
      841 cgcccgacca ccgcttgatc cgttacttca agccgtagct caggacgcgt tgaggcgctc
      901 gtcgagcagc tggttgagcg tgaccgggtc gccctgaccc ttcatctcct tcatgagctc
      961 gcccatgagg aagccgcgct tcttcttctt gcccttgtcg tcggcggccc ggaactccgc
     1021 gaccgcgtcc gcgttgcgga caagcaccgc gtcgacgacg gccgcgagct cgccggtgtc
     1081 ggtgacctgc ccaaggcccc gcgcttcgac gatgtcgcgg ggccacttgt cttcgcgcag
     1141 cgattcgtcg agcacgtcct tgccctggtt gcgcgagatc ttgccgtcgg cgacgaggct
     1201 caccagctcg ccgagcatct ccggcgacag caccgcgggc gccaggttcg tcttgttcag
     1261 gtatgccagc acttcctgca ccgcccagtt cacgacgtcc ttcggcgtgc cggcagtgag
     1321 ggcggcgacc gccttggccg cgtagtcgct gagcccgggc gtgctcacga gcacgcgcgc
     1381 gtcgtcgggc ttgatgcccc actcctcgac gagccgcgcc cggtgcgcgg ccggaagctc
     1441 cggcatcgtc tcgtggacgc gcgtgcgcat ctcgtcggtc ggcgcgaccg gcaccagatc
     1501 gggctcgggg aagtagcggt agtcgtgcgc ctcttccttc gaacgacccg agaccgtgcg
     1561 gccgcgcgct tcgtcccagt gccgcgtctc ctgcacgatg tcgtcgccga actcacgcgc
     1621 cgtcacctgc cgcttcgcct cgtgctcgat cgccttcgtc aacgaccgca gcgagttgag
     1681 gttcttgatc tcgcagcgcg tgccgaactc ggtcgagccc tcgggccgca ccgacacgtt
     1741 cgcgtcgatg cgcagcgacc cttcttccat cttgacgtcg gacacaccga tcgcgagcaa
     1801 cgtcgcgcgc agctcttcga cgtagcgctt ggcctgctcg gccgagcgga tgtcgggctc
     1861 cgagacgatc tcgaggagcg ggacgccggc gcggttgtag tcgacgagcg aatggtcggc
     1921 gtcctggatg cggccactcc caccgacgtg caacgacttc ccggtgtcct cttcgaggtg
     1981 cgcgcgggtg atgccgatgc gcgtgtcgtc gacgtcgagg aagccgtcga tggtgatcgg
     2041 ctcgtcgtac tgcgacgtct ggtagtcctt cggcatgtcg ggatagaagt agttcttccg
     2101 cgcgaacacc gactgcgccg gtaccttcag gttgagcgcc tcggcgacgc gcagcgcgta
     2161 ctcgacggcc ttctcgttca gcaccggcag cgagccgggc agcccgaggc acacggggca
     2221 cacgttggtg ttcggttcgg tgccgaactc gttcgggcat ccgcagaaga gcttgctcgc
     2281 cgtcgagagc tcgcagtgca cctcgagccc gacgacggtt tcccaggtcg tcacggctgc
     2341 cagactacgg gacatggctg ctgcgcccga cccgaacgcc tttgcgacgc accgcgccga
     2401 cgtccgcaac ggcgtcacga tcgcgtacgt gcgagaaggg gtcggcggca cgcccctgct
     2461 gctcctgcac ggctacccgg agaccaagcg catctggtgg cgcaacatcg ggccgctcgc
     2521 ggcggccggg ttcgaggtga tcgcacccga ctaccgcggg cacggcgact cgagcctcgc
     2581 gcccgacaac ttctacgaca tcgccgcgtt cgccatcgac tgctacacgc tcgtgcacga
     2641 cgtgctgggg cacgagtcgt gcgcggtcgc gggtggtgac gtcggcggca tcgtgctgtt
     2701 cgacatcggc ctgcgctacc ccggcttcgt gacgcgtcag gtcttgttca acacgcttcc
     2761 gccgccgctg tacgacgtct accaagccgc gggactcggt cccgacgacg accgcgcgac
     2821 gcgcgcgacc gccgactact tccggcgcca gtcgaccgag cccgacgtct tgctcgcgga
     2881 gctcgacacg cccgcgcgcc gccaggcttg ggtggcgccg atgtacggcc accggttgtg
     2941 gggcacgcca cacgcgttca ccgacgagga agtcgcattt cattccgagc cgttcgcgga
     3001 tgcggccaag ttgcgcgcga gctggggcgt ctacgagcaa gccgccgggc aacgcccgat
     3061 ggaagacgtg cccaagctgt tcgaaccgac gccggtcccc acgatggtgc tctacggcac
     3121 cgaggaccac gtcgtaccga agtcgttccc gtggaaggcc gcggccgcgt gcctcgactg
     3181 caccggcccg ctgttcgtgc ccaacgctgg ccacttcctg cagtgggagt cggccgacac
     3241 gttcaacaag atcacggcgg cgttctgccg ctaagcgcta ggcgagcagc ggccgcacct
     3301 gggtcgcgaa ccggtggagc tgctcgactt cgtcggcgac gggccagatg aacacttgtt
     3361 gcacgccggc atcggcgaac gcgcgcagct tctccgcgaa gcgcaccggt gacccgatcg
     3421 gcagccgttc gcgcaacacc gcttcgggcc ggtgcacggt cggcacgacg cgttccacga
     3481 gcacgcgctc cgcttcggca tcgtcgtcgg tgatgtagca ccacatggtc gccagtgcat
     3541 tcgggaaccc gtcggcgcgc gcgccgagga gcgactgcaa ctgctgccac gcgtcggcga
     3601 acaacgcggg cgtcgtgttg tacgcggacg ccagccaacc gtccgcgagg cgcgcgacgc
     3661 gccgcagccc ggccgcggag ccccagctcc cgatccacac gggcggtccg tccggctgca
     3721 ccggcggtgg cgcgagccgc gtccactgcg cgcgcagttc gagcacggac gcctcgaagc
     3781 gcggccaccg ctcgccgaaa tcgatgccga ccgtcgcgta gtcgtcggcc gacgagcccg
     3841 gtcctacacc ggcgatcacc cgcccacccg acagccggtc gatcgcggcc aacgacttgg
     3901 ccagcgggac cggcccgcgc acgacggaaa gcgcgacggt cgtcacgagg ttcatcgtcc
     3961 ccgtgtgctc gacgaccgcc ccgagcgcgg cgagaccgtc gagccacggc accgagaaca
     4021 ccatgtggtc gttcgccgag accgcgtcga aacccaggtc gcgagcggcg cgggcgtagg
     4081 cgacgaggtg ttcgcgcgag taggggttcc cgccgaagtc catcagcggc aggtgcgccc
     4141 cgacccgcat ggcacatgcg tcgatagccc accgtgacgg tgcgctatcg acgcacgtcc
     4201 tccagggcgc gggcggcgcg gaacatcttc tcctcgccca gcgcgtcggc gagcacttgc
     4261 acgccgacgg ggagcccgtg cgcgccggtg ccgaacggca cggagatgcc cgggtggccc
     4321 gcgaggttcg tcgggatcgt gcagacgtcg cagaggtaca tcgcgagcgg atcggcggtc
     4381 ttggcgccgg cttcgaacgc gaccgtcggc gacgtcggcg agagcagcac gtcgaacgtc
     4441 tcgtacgccc gcgcgaagtc gcgcaggatc agcgtacgca cgcgctgggc ctggccgtag
     4501 tacgcgtcgt agtagcccgc cgacagcgcg tacgtgccga gcatgatgcg gcgcttcact
     4561 tcgcggccga agcccgcgtc gcgcgtcttg gcgttcatct cggcgacgtc gttgccgtcg
     4621 acgcgcagtc cgtagcgcac accgtcgtag cgcgccaggt tcgacgacgc ttcggccggc
     4681 gcgatcaggt agtacgccga gagcccgtac gtggccgacg ggatcgagac gcggtcgacc
     4741 ttcgcacccg ccgactccaa tgcctgcgcg gcgccgtcga cc
//
LOCUS       DASUGA010000526         2980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_471308, whole genome shotgun sequence.
ACCESSION   DASUGA010000526 DASUGA010000000
VERSION     DASUGA010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471308"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VFX21_10405"
     CDS             <1..910
                     /locus_tag="VFX21_10405"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5096418.1"
                     /translation="HVLRYGPTRRQLTGIQAVFSDGAVVTRLDGLEKDNTGYDLASLL
                     CGSEGTLAIITAARFRLHPPPRATVTALCAFEAVGEAVAAAGTVRRTLPSVNAIELFV
                     QSGLDLVCERLQLTAPFAAAHAAYLLVEAAADSDPTDALGRAIESCDGVRDVAVATDG
                     PSARALWRYREGHTEAINLLGAPHKLDVALPADRLARFIETVTQRVHAVAPDATVWLF
                     GHAGDGNVHVNVTGVEPDDDSVSAAVFELVAELHGSISAEHGIGAAKRAYLKLVRSQT
                     EIAAYRAIKRALDPTGTLNPHVLLPG"
     gene            complement(1096..1695)
                     /locus_tag="VFX21_10410"
     CDS             complement(1096..1695)
                     /locus_tag="VFX21_10410"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5096419.1"
                     /translation="MYTDDQRSWQDKFDSRRIADRIDSLLVADHIDEHARRFIESRDM
                     FFLATVDADGHPQCSYKGGDPGFVRVVDERTLAFPSYDGNGMFLSLGNISAHAAVGML
                     FIDFEHPNRLRVNGTASVDENDPLLAEMPGARLVVRVHTTHVFPNCNRYIHSMQLVER
                     SKFVPKADADAPIPDWKRMSWAQDQLPENDPARDAEPLA"
     gene            complement(1705..>2980)
                     /locus_tag="VFX21_10415"
     CDS             complement(1705..>2980)
                     /locus_tag="VFX21_10415"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEX5096420.1"
                     /translation="TKLAVEGSLPKELNGLYVRNGSNPKSGWAPHWFLGDGMVHGVML
                     ENGSASWYRNRYVETALLAAGGGLTASGAPGGAASLSNVSLVHHAGKLLTLGEVGFPY
                     EISPKDLSTVGAYDFAGKLAGSMTAHPKIDPATGHMHFFGYNFSQPYLAYHVADKTGA
                     LISTQAVPVKSSTMIHDFAITDRDVIFWEMPVLFDMELAVKMVSEEKSTIMPYVWTPS
                     YGSRVGIMPLGGPASEIRWVEIENCYVFHGMNAFRDGDDVVVDVSRLAKVFDGSAIGP
                     PPSLHRWRINTAGKTLTFRDEQRTDTVADLPSIDKRYTGRPYTHGWRVVTRESSETNV
                     DLGGVVHINATTNAETQWNPGLRYSSGEWLFVPTGDAEGEGVIMTFVHDKADDKTSLT
                     VLDARDVAAGPLARIELPQRVPYGFHATWVPA"
BASE COUNT          492 a          978 c          978 g          532 t
ORIGIN      
        1 tcatgtgctg cgctacgggc cgacccggcg acaactcacc ggcatacaag cggtgttcag
       61 cgacggcgcg gttgtgacgc gcctcgatgg cctcgagaag gacaacaccg gttatgacct
      121 cgcgagcttg ttgtgcggca gcgagggcac gctcgcgatc atcaccgctg ctcgcttccg
      181 ccttcacccg cccccgcgcg cgaccgtgac ggcgttgtgc gcgttcgaag ccgtcggcga
      241 ggccgtcgcg gcggccggca ccgtccggcg aaccctgccg tcggtgaacg ccatcgagct
      301 gttcgtgcag tccggcctcg atctggtctg cgaacggctg cagctcaccg cgccgttcgc
      361 ggctgcgcac gccgcctatc tccttgtaga ggcggcggcc gactcggatc cgacggacgc
      421 gttgggcaga gcaatcgaga gctgcgacgg cgttcgcgac gtcgcggtcg cgaccgacgg
      481 accctcggcg cgcgcgttgt ggcgctaccg cgagggccat accgaagcca tcaatctgct
      541 cggcgccccg cacaagcttg acgtcgcgct gcccgcggac cgcttggcgc gcttcatcga
      601 aacggtgacg caacgggtac atgcggtcgc cccggacgcg accgtgtggt tgttcggtca
      661 cgcgggtgat ggcaacgtgc acgtaaatgt cacgggtgtc gagccggacg acgattcggt
      721 cagtgctgcc gtcttcgagt tggtcgccga gctgcacggt tccattagtg cggagcacgg
      781 aatcggcgcc gccaagcggg cgtatctcaa actcgtacga tcgcagaccg agattgccgc
      841 gtaccgcgca atcaagcgag cgctcgaccc cacggggaca ctgaatcctc acgtgttgct
      901 gccgggttga gccgctccga ctgtcaagaa cgcacgttcc ggacactccg cttgacttgc
      961 gcacaaatcc ggcaagagac tgtggccgta gctgcgatct gtcacaccgg cccgtggcgc
     1021 cccgcgccgg tgtggctggc tcgggtcgtc ggccggtcgg accgcccgcc gccgcccggc
     1081 gacccgagcg ccctttcaag ccaaaggttc ggcgtcgcgc gcgggatcgt tctccgggag
     1141 ttggtcctgc gcccaactca tgcgtttcca gtccggaatc ggtgcgtcgg catcggcttt
     1201 cggaacaaac tttgaacgtt cgaccaactg catcgagtga atgtaacggt tgcagttcgg
     1261 gaagacgtgc gtcgtgtgaa cgcgcacgac gagtcgcgcg ccgggcatct ctgcgagcag
     1321 tggatcgttc tcgtcgaccg acgcagtgcc gttgactcgt agccgattgg gatgttcgaa
     1381 gtcgatgaac aacatcccga ctgcggcgtg cgcgctgatg ttgccgagtg agaggaacat
     1441 gccgttcccg tcgtagctcg ggaacgcgag cgtgcgttcg tcgacgacgc gcacgaaccc
     1501 ggggtcgccg cccttgtacg agcactgcgg atgaccgtca gcgtccaccg tcgcgaggaa
     1561 gaacatgtcg cgcgattcga tgaagcgccg tgcatgctcg tcgatgtggt cggcgaccag
     1621 cagcgaatcg atccggtcgg cgatgcggcg cgagtcgaat ttgtcctgcc aggaccgctg
     1681 atcgtcggtg tacacggcgc gaaactacgc cggaacccac gtcgcgtgga agccataggg
     1741 cacccgctgc ggaagttcga tccgggcgag cggcccggcc gcaacgtcgc gtgcgtcgag
     1801 gaccgtgagc gaggtcttgt cgtcggcctt gtcgtgcacg aaggtcatga tcacgccctc
     1861 gccttcggcg tcgccggtcg ggacgaacag ccactcgccg gacgagtagc gcagccccgg
     1921 gttccactgc gtttcggcgt tggtcgtcgc gttgatgtgg acgacaccgc cgaggtcgac
     1981 gttcgtttcg ctgctctcgc gcgtgacgac ccgccagccg tgtgtatacg gacggccggt
     2041 gtagcgcttg tcgatcgacg gaaggtcggc gaccgtgtcg gtgcgctgct cgtcgcgaaa
     2101 tgtcaacgtc ttcccggcgg tgttgatccg ccaccggtgc agcgacggcg gtggcccgat
     2161 cgccgaaccg tcgaagactt tcgccaagcg actcacgtcg acgacgacgt cgtcgccgtc
     2221 gcgaaacgcg ttcatgccgt ggaagacgta gcaattttcg atctcgaccc accggatctc
     2281 ggacgccggt ccgccgagcg gcatgattcc cacacgcgag ccgtagctgg gcgtccacac
     2341 gtacggcatg atcgtcgact tttcctcgct gaccatcttc accgcgagtt ccatgtcgaa
     2401 caggaccggc atctcccaga agatcacgtc gcggtcggtg atcgcgaagt cgtggatcat
     2461 cgttgacgac ttcaccggaa ccgcctgggt cgagatcaac gctcccgtct tgtcggcgac
     2521 gtgatacgcg agatacggct gcgagaagtt gtatccgaag aagtgcatgt gcccggtcgc
     2581 cgggtcgatc ttcggatgcg ccgtcatgct tccggcgagc ttccccgcga agtcgtacgc
     2641 gccgaccgtc gaaagatcct tcggcgagat ctcgtagggg aacccgacct ccccgagcgt
     2701 cagcagcttg ccggcgtgat gcaccaacga gacattgctc aagctggccg cgccgccggg
     2761 cgcgccgctg gccgtcaggc cgccgcccgc ggcaagcagg gcggtttcga catagcggtt
     2821 gcggtaccag ctcgccgagc cgttctcgag catgacgccg tgcaccatgc cgtcaccgag
     2881 aaaccagtgc ggcgcccacc cggacttggg attcgagccg ttccgaacat agaggccgtt
     2941 cagttccttt ggcagcgaac cctcgacggc gagcttcgtc
//
LOCUS       DASUGA010000527         4037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1553027, whole genome shotgun sequence.
ACCESSION   DASUGA010000527 DASUGA010000000
VERSION     DASUGA010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553027"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VFX21_10420"
     CDS             <1..307
                     /locus_tag="VFX21_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096421.1"
                     /translation="PYTFLGPRPLHIAPEATVRTAHSVTAFRNLDVLTLLGGAASAMR
                     TGKFLRNHRDVVMRHDVDRITVAGRGPFPYQADGDDLGDAEHLEVGFEPDALTIVLP"
     gene            complement(311..1012)
                     /locus_tag="VFX21_10425"
     CDS             complement(311..1012)
                     /locus_tag="VFX21_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX5096422.1"
                     /translation="MPDTVLILGHRGASHDARENTLEAFALARAQGADGVELDVRRTA
                     DGELVVHHDPHVPGVGLLAQLPFARLREEAPFVPTLGEALAACEGMLVNVEVKCLPWE
                     PDADHESQVAKASADLIRAMGAHTVMSSFHFAAIDALRTYAPEIPTGFLVHGQDLAFT
                     RDLVKMRGHEWLHPDRGSMLATDTAAVVRTCHEENLRVNVWTVDDPDEMRRLADAGVD
                     GIITNVPAVAVSVLR"
     gene            1191..1493
                     /locus_tag="VFX21_10430"
     CDS             1191..1493
                     /locus_tag="VFX21_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX5096423.1"
                     /translation="MALTWIRTHDWDADAWRNKASCRDTSPELFFPIGTTGLALDQID
                     AAKRVCGQCPVTAECLEFALATNQEAGVWGGLTEEERRRLRKGWTTDVAVSEARVG"
     gene            complement(1592..3055)
                     /locus_tag="VFX21_10435"
     CDS             complement(1592..3055)
                     /locus_tag="VFX21_10435"
                     /inference="COORDINATES: protein motif:HMM:NF023702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5096424.1"
                     /translation="MTTVLELARSRTALAGEDLAHIERLVATWRPMADLSFSDLLLLA
                     PIVGEEGHRFVVLAQVRPVTGQTNYPQDLVGSVVDEVERPLLTRCWRTGDIVEGDTTA
                     LAAKDRVHVHCIPMRRDGRLIGVLTRESAISFTRRLGELERAYLEVFERFARMLAEGS
                     FPYEVDEMEVESAPRVGDGVIVIERDTRVRFASPNAVSSLHRMGIHAYAQGQPLGAVG
                     FDDAAAREAMATRLPIAEELERGDISVLSQVLPLCEDGVALGAIILLRDVSDLRRRDR
                     MLMSKDATIREIHHRVKNNLQTIASLLRLQTRRLRSPEAKAALAESERRIRSIAIVHE
                     ILSREPGDFVELTEIVRPLARLVEETASSDDQHIRFSVEGDAGELPGEVATPLAVVLN
                     ELMQNAVDHAFPEGIASGKVAVVMSRDDGHVTVEVIDNGAGLPDEFSIDGGSRGLGLS
                     IVHALVTSELAGSIEMHSNGGTTVRVRVPVEMPRVEL"
     gene            complement(3140..>4037)
                     /locus_tag="VFX21_10440"
     CDS             complement(3140..>4037)
                     /locus_tag="VFX21_10440"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEX5096425.1"
                     /translation="QSWDDLVLRHAPDEETAYRILENRLYNNLTARFVQSHDYIAMER
                     LYEIHATGEYDLIVIDTPPTRNAIDFLEAPARMADFFSSRLLRWLTLPYRVGGKRGNR
                     VLSVASKPFYQIADRILGSAFLEDIAEFFLNFQSMYDGFVARSKAVERLLHDRRTTFT
                     VVTTLEAAPLHEAERFCKELIARKFHLGALVLNKTLPDYLLSAQGAAAATAFVDHAGP
                     IAEELASLDNPALADTARTARVLRTIGDSYRNFSVVALRESELRNELAHTPDVIARVP
                     TLEGDIGDLEGLVAIGHWLFAD"
BASE COUNT          656 a         1380 c         1383 g          618 t
ORIGIN      
        1 gccgtacacg ttcctcggtc cgcggccgtt gcacatcgcg ccggaggcga ccgtgcgcac
       61 ggcacactcg gtgacggcct tccggaacct cgacgtgctg accctgctcg gcggcgccgc
      121 ctccgcgatg cgaacgggca agttcctccg caatcacagg gacgtcgtaa tgcgccacga
      181 cgtcgaccgc atcacggtcg ccgggcgcgg cccgttcccc taccaggccg acggcgacga
      241 cctcggcgac gccgagcacc tcgaagtggg cttcgagccc gacgcgctga cgatcgtctt
      301 gccctagtcg ctaccgcaac accgaaacgg cgaccgcggg aacgttcgtg atgatgccgt
      361 cgacacccgc gtcggcgagc cggcgcatct cgtcggggtc gtcgacggtc cacacgttca
      421 cgcgcaagtt ctcttcgtgg cacgtgcgca cgacagccgc ggtgtcggtg gcgagcatcg
      481 aaccgcggtc gggatgcaac cactcgtgcc cgcgcatctt cacgaggtca cgcgtgaacg
      541 cgagatcctg gccgtgcacg aggaacccgg tcgggatctc gggtgcgtac gtgcgcaacg
      601 catcgatcgc ggcgaagtga aacgacgaca tcaccgtgtg cgcacccatc gcacggatga
      661 gatcggcgga cgccttggcc acctgactct cgtggtcggc gtcgggctcc cacggcaggc
      721 acttcacctc gacgttgacg agcatccctt cgcacgcggc cagcgcctcg ccgagcgtcg
      781 gcacgaacgg cgcttcctcg cgaaggcggg caaacggcag ctgcgcgagc agcccgactc
      841 cgggcacgtg cgggtcgtgg tggacgacca gttcaccgtc ggcggtgcgg cgcacgtcga
      901 gctccacgcc gtcggccccc tgggcccgcg cgagcgcgaa cgcctcgagt gtgttctccc
      961 gcgcgtcgtg cgacgcaccc cgatgaccca ggatcaacac ggtgtccggc accccgtgag
     1021 gctacgcgca caattcggca acgtgttcca tttctgcgcg caaaacccct ggtcaggacc
     1081 cttgcaacaa gcttgcacgg ccgttaccct gccggttccg gacaccgggc gcacacacac
     1141 ggccgacgca ggtggggagt cggcggtgcg acccgagggg ggaagaactt gtggctctga
     1201 cttggattcg tacgcacgac tgggacgcgg acgcatggcg caacaaggcg tcgtgccgtg
     1261 acaccagtcc ggagctcttc ttcccgatcg ggacgacggg gctcgctctc gaccagatcg
     1321 acgcggccaa gcgtgtttgc gggcagtgcc ccgtcacggc cgagtgcctc gagttcgcac
     1381 tcgcgacgaa ccaggaagcc ggcgtgtggg gtggcctcac cgaagaggag cgccgacgcc
     1441 tgcgcaaggg ctggacgacc gacgtcgcag tcagcgaggc acgcgtcggc tgacgctcgg
     1501 gcgacagcga gcgccgcgag cggtgcgtcg cccggaaccg gaagaggcgt cggcggagcc
     1561 ggcatagggg cgcggggcga agctcccgct gtcacaactc gactctgggc atctcgaccg
     1621 gcacgcgcac gcgtacggtc gtgccgccgt tgctgtgcat ctcgatcgag ccggccagct
     1681 cgctcgtgac cagcgcgtgc acgatcgaca aaccgagtcc gcgcgagccc ccgtcgatcg
     1741 agaactcgtc gggcagcccg gcgccgttgt cgatgacctc gacggtgacg tgaccgtcgt
     1801 cgcggctcat gaccaccgcg accttgcccg acgcgatgcc ttcggggaac gcgtggtcga
     1861 ctgcgttctg catcagctcg ttgagcacga ccgcgagcgg cgtcgccacc tcgcccggca
     1921 gctcgcccgc gtcaccttcg accgagaacc ggatgtgctg atcgtcggac gacgcggtct
     1981 cctcgaccaa ccgcgccaac ggccgcacga tctcggtcaa ctcgacgaag tcacccggct
     2041 cccgcgacag gatctcgtgg acgatcgcga tcgagcggat gcggcgctcc gactcggcca
     2101 gcgcggcctt cgcttccggc gaccgcagcc gccgggtctg gagccgcaac agcgacgcga
     2161 tcgtctgcag gttgttcttc acccggtggt ggatctcgcg aatcgtcgcg tccttcgaca
     2221 tcagcatgcg gtcgcggcgc cggaggtcgc tgacgtcacg gagcaggatg atggcgccca
     2281 acgcgacgcc gtcctcgcac aacggcagga cttgtgacag caccgagatg tcgccccgct
     2341 cgagctcttc ggcgatcggg agccgggtcg ccatcgcttc gcgcgcggcg gcatcgtcga
     2401 aaccgacggc gcccaacggc tggccttgcg cgtacgcatg gatgcccatg cggtgcagtg
     2461 agctgacggc gttcgggctc gcgaaccgga cacgggtgtc gcgctcgatg acgatgacgc
     2521 cgtcaccgac gcgcggcgcg ctctcgacct ccatctcgtc gacctcgtag ggaaacgagc
     2581 cttcggcaag catgcgcgcg aagcgttcga acacttcgag gtacgcgcgt tccagctcac
     2641 cgaggcggcg tgtgaacgag atcgcggact ctcgcgtcag cacgccgatc aaccggccgt
     2701 cgcggcgcat cgggatgcag tgcacgtgca cgcggtcctt ggccgcgagc gcggtcgtgt
     2761 cgccttcgac gatgtcgccc gtccgccagc agcgcgtcaa cagcggccgt tcgacctcgt
     2821 cgacgacgct gccgacgagg tcttggggat agttcgtctg cccggtgacc gggcggacct
     2881 gggcgagcac gacgaagcgg tggccctcct cccccacgat cggcgccagc agcaacaggt
     2941 cggagaagga gaggtcggcc atcgggcgcc aggtcgcgac gagccgctcg atgtgcgcga
     3001 gatcctcgcc ggccaaggcc gtgcgcgatc gggccagctc gagaacggtc gtcactcccc
     3061 gaatggtacg gccggatcgc gcattctttg tcaccctggc ccgcggagag cgggccacat
     3121 tgaccgagaa cgggcggcgt cagtcggcga agagccagtg gccgatcgcg acgaggccct
     3181 cgaggtcgcc gatgtcgcct tcgagggtcg gcacgcgggc gatgacatcc ggcgtgtggg
     3241 ccagctcgtt gcgcaactcc gactcgcgca gcgcgacgac cgagaagttg cggtacgagt
     3301 cgccgatcgt gcgcagcaca cgggccgtgc gcgccgtgtc ggcgagcgcc ggattgtcga
     3361 gcgatgccag ctcctccgcg atgggtccgg catggtcgac gaacgcggtc gcggcagccg
     3421 caccttgggc gctcaggaga tagtcgggca gcgtcttgtt gagaacgagc gcacccaagt
     3481 ggaacttgcg cgcgatcagc tccttgcaga accgctcggc ctcgtgcaac ggcgccgctt
     3541 cgagggtggt gacgacggtg aatgtcgtgc gccgatcgtg cagcaaccgt tcgaccgctt
     3601 tggaccgcgc gacgaacccg tcgtacatcg actggaagtt caagaagaac tcggcgatgt
     3661 cttcgaggaa cgcgctgccg aggatgcggt cggcgatctg gtagaagggc ttgctcgcga
     3721 ccgacagcac gcggttgccg cgcttgccgc cgacccgata cggcagcgtg agccaccgca
     3781 gcagccggct gctgaagaag tcggccatgc gcgccggcgc ttcgaggaag tcgatcgcgt
     3841 tgcgcgtcgg cggggtgtcg atgacgatca ggtcgtactc gcccgtcgca tggatctcgt
     3901 agagccgttc catcgcgatg tagtcgtggc tctgcacgaa ccgtgcggtg aggttgttgt
     3961 acaagcggtt ttcgaggatg cggtacgcgg tctcctcgtc gggcgcgtgc cgcaacacga
     4021 ggtcgtccca cgactgc
//
LOCUS       DASUGA010000528         2106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_705123, whole genome shotgun sequence.
ACCESSION   DASUGA010000528 DASUGA010000000
VERSION     DASUGA010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705123"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1999)
                     /gene="treS"
                     /locus_tag="VFX21_10445"
     CDS             complement(<1..1999)
                     /gene="treS"
                     /locus_tag="VFX21_10445"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEX5096426.1"
                     /translation="MTRWYEDAVIYELHVRAFFDSDNDGIGDFVGLTRKLDYLQDLGV
                     TAIWLLPFYPSPLKDEGYDISDYRGINAAYGTLRQFRRLLDEAHRRDLKVITELVLNH
                     TSDQHPWFQRARRASRTSKYRDWYVWSDDPNQYRDARIIFGDFEQSNWAWDPVAGQYY
                     WHRFYSSQPDLNFANPDVRREMLKTVEYWLDLGVDGLRLDAVPYLFEREGTSCENLPE
                     THEFLRELRARVDARFEDRLLLAEANQWPEDAIAYFGAGDECHMAFHFPLMPRLFMGL
                     RMEDRFPIIDILRQTPRIPDGCQWAVFLRNHDELTLEMVTDEERDYMYRSYARDPAMR
                     VNLGIRRRLGPLLHNNRQKIELMHGLLCSLPGAPVLYYGDEIGMGDNVYLGDRDGVRT
                     PMQWSADRNAGFSGANPQKLYLPVIIDPEYNFETVNVAAQLDNPESLLWWIRRLIALR
                     KRYPVFGTGDLQILSPENHRVIAFVRRERTTPSDNGQQRPQQVLVVANLSRYPQYVEL
                     DLSEFRGSRPTELFGQTTFPTIGDLPYLLTLGPYAFYWLSLEPDPAERASGDSDDLPV
                     ISVAGDWAFTGGRARIERLLPALLRQRRWFGAKGRVMTAAVIDDVAEVPIRHYWSEDE
                     QLAAPEPVRVLFVRVEYVHGDAETYVLPLAFVPGDAGARLVA"
BASE COUNT          364 a          688 c          710 g          344 t
ORIGIN      
        1 cggcgacgag gcgcgcaccg gcgtcgccgg gtacgaacgc gaggggcaag acgtaggtct
       61 ccgcgtcgcc gtgcacgtat tcgacgcgca cgaacaagac ccggaccggc tccggagccg
      121 cgagttgttc gtcctcgctc cagtagtgcc ggatcggcac ctcggccacg tcgtcgatga
      181 ccgcggccgt catcacccgg cccttcgcgc cgaaccatcg acgttgccgg agcagcgccg
      241 gcaacagccg ctcgatgcga gcccgcccgc cggtgaacgc ccagtcgccg gcgacggaga
      301 tcaccggcag gtcgtcgctg tcgccgctcg cgcgctccgc cggatccggc tccagcgaca
      361 gccagtagaa cgcgtacgga ccgagcgtca acaggtacgg caggtcgccg atcgtcggga
      421 acgtcgtctg cccgaacaat tcggtgggcc gtgatccacg gaactcggag agatcgagct
      481 cgacgtactg cgggtaccgc gacaggttcg cgaccacgag cacctgctgc gggcgctgct
      541 gcccattgtc ggatggcgtc gtgcgttcgc ggcggacgaa cgcgatcacc cgatggttct
      601 cgggcgagag gatctgcagg tcgccggtgc cgaagacggg atagcgcttc cgcaacgcga
      661 tcagtcgtcg tatccaccac agcaacgact cgggattgtc gagctgcgcg gcgacgttga
      721 ccgtctcgaa gttgtattcc ggatcgatga tgaccggcag gtacagcttc tgcggattgg
      781 ctcccgagaa gccggcgttg cgatccgcgc tccattgcat cggcgtccgc acaccgtcgc
      841 ggtcgccgag atagacgttg tcacccatgc cgatctcgtc gccgtagtac agaaccggcg
      901 cgcccggcaa cgagcacagc aagccgtgca tgagctcgat cttctgccgg ttgttgtgga
      961 gcagcggacc gagccggcgg cggatcccga ggttgacacg catcgccgga tcacgcgcgt
     1021 acgagcggta catgtagtca cgctcttcgt cggtcaccat ctcgagcgtc agctcgtcgt
     1081 ggttccgcaa gaacacggcc cactggcagc cgtcggggat ccgcggcgtc tgccgcagga
     1141 tgtcgatgat cgggaaccgg tcctccatgc ggagacccat gaacaagcgc ggcatgagcg
     1201 ggaagtggaa cgccatgtgg cactcgtcgc cggcgccgaa gtacgcgatc gcgtcctcgg
     1261 gccactggtt cgcttccgcg aggagcagac ggtcctcgaa tcgcgcgtcg acgcgcgccc
     1321 gcaactcacg caggaactcg tgcgtctccg gcaggttctc gcagctggtg ccctcccgct
     1381 cgaacaggta cggcaccgcg tccaaccgca gcccgtcgac gccgagatcg agccagtact
     1441 cgacggtctt cagcatctcg cgacgcacgt cggggttcgc gaagttgaga tcgggctgcg
     1501 acgaatagaa gcggtgccag tagtactggc ccgcgaccgg gtcccacgcc cagttcgact
     1561 gctcgaagtc accgaagatg atgcgcgcgt cgcggtactg gttcggatcg tcgctccaca
     1621 cgtaccagtc gcggtacttg ctcgtgcgcg aagcgcgacg cgcgcgctgg aaccacgggt
     1681 gctggtcgga ggtgtggttg agcacgagct cggtgatcac cttcaggtcg cgccgatgcg
     1741 cttcgtcgag caaccgccgg aactgccgca gcgtgccgta cgcggcgttg atgccgcggt
     1801 agtcggagat gtcgtagccc tcgtccttga gtggcgacgg gtagaagggc aacaaccaga
     1861 tcgcggtgac gcccagatct tgcaggtagt cgagcttgcg ggtgagaccg acgaagtcgc
     1921 cgatgccgtc gttgtcgctg tcgaagaacg cgcgcacgtg cagctcgtag atgacggcgt
     1981 cctcgtacca gcgcgtcacg agatgtgctc tcgttcaagg ctgaattcga ggctgaagat
     2041 gtgcgccggc accgcgccgg gatcgagccg gacgaagttg gcgcccgcgt gccacgggta
     2101 ccgcgc
//
LOCUS       DASUGA010000529         3022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_305842, whole genome shotgun sequence.
ACCESSION   DASUGA010000529 DASUGA010000000
VERSION     DASUGA010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305842"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /gene="eno"
                     /locus_tag="VFX21_10450"
     CDS             <1..447
                     /gene="eno"
                     /locus_tag="VFX21_10450"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833573.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX5096427.1"
                     /translation="VSIEDGMAEDDWGGWAALTQRVGQNVQLVGDDLFVTNVERLSMG
                     IERRVANSILVKVNQIGTLTETLDTMQLASRAGYTAVMSHRSGETEDATIADLAVATN
                     CGQIKTGAPARSDRVAKYNQLLRIEGELGNAAAYLGARAMEGGARR"
     gene            444..749
                     /locus_tag="VFX21_10455"
     CDS             444..749
                     /locus_tag="VFX21_10455"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX5096428.1"
                     /translation="MTHWGRARVALIALGVATFLFLFVFPTRSYLDQRGRVGAAQHDV
                     NVLREQNDKLQAEALQLQTPAAIERMAREQFHMVKPGEQVYNVSPAPTTTSTTTPAP"
     gene            760..1218
                     /locus_tag="VFX21_10460"
     CDS             760..1218
                     /locus_tag="VFX21_10460"
                     /inference="COORDINATES: protein motif:HMM:NF016314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HEX5096429.1"
                     /translation="MPTHEHDVASLTQLLGRPPRAEFEVVVRSDAGEPAVIRNAPLLD
                     DGTPMPTRFWLVDPALSLRVARLESDGGVRAAEAAVDAGALQAAHDAYASERDAAIAP
                     DHAGPRPHGGVGGTRRGVKCLHAHYAYFLAGGDDPVGRWVAQQLAEEQLA"
     gene            1215..2135
                     /locus_tag="VFX21_10465"
     CDS             1215..2135
                     /locus_tag="VFX21_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006348845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX5096430.1"
                     /translation="MRRVAAIDIGTNSTRLLVADVDGTGRDAKLVPVDRRTQITRLGQ
                     GVHDARRLHPDAIERTLAVLRDYRVVLDDLGVEAVRATATSASRDSSNRDEFFQPATQ
                     IIGVEPELLTGEEEARLEYLGATASLAAPEPYLLFDVGGGSTEFIAGRAEPEALTSID
                     IGCVRLTEQYLHSDPPAAEELSAAHSVVRDYLADVDRLVPGAAGAATLIGTAGTVWTM
                     AAVELGVDAARSDLIDHFRLTREAAEDVFRTLATEPIARRRHNPGLEPGRVDVIVGGA
                     IVVVSVLRHWGFNELLVSEADILDGLARTI"
     gene            2290..2682
                     /locus_tag="VFX21_10470"
     CDS             2290..2682
                     /locus_tag="VFX21_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096431.1"
                     /translation="MELLAFERALADLSVPEIRLIALDLVSANECTADEVARTRSVLV
                     IEQTLRRTHRLHSAASAALEAATTVQEVAERGKVSLPDDDVTRVARAAAQLARGLIIG
                     EAPGVDDALRVLGRGWYRLPCLDELRVA"
     gene            2769..>3022
                     /locus_tag="VFX21_10475"
     CDS             2769..>3022
                     /locus_tag="VFX21_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX5096432.1"
                     /translation="MGPGPSNPYPEVMEAFQRPVLGHLDPDFLVVLDEIGDRLRAVFR
                     TQNELTLPISGTGSAGMEACFVNLVEPDDTVISGVNGVFG"
BASE COUNT          495 a         1031 c         1019 g          477 t
ORIGIN      
        1 gtttcgatcg aagacggcat ggccgaggac gactggggcg gctgggccgc gttgacgcag
       61 cgagtcggcc agaacgtgca gctcgtgggc gacgatctgt tcgtgaccaa cgtcgagcgg
      121 ctttcgatgg gcatcgaacg cagggtcgcc aactcgatcc tcgtcaaggt caaccagatc
      181 ggcacgctga ccgagacgct cgacacgatg cagctcgcga gccgggcggg ctacaccgcg
      241 gtgatgtcgc accgcagcgg cgagaccgag gacgcaacga ttgccgacct cgcggtggca
      301 acgaattgcg gccagatcaa gaccggcgca cccgcgcgca gtgaccgcgt cgcgaagtac
      361 aaccaattgc tccgcatcga aggcgaactc ggcaacgcgg ccgcctacct cggcgcacgc
      421 gcgatggaag gcggcgcgcg ccgatgacgc actggggtcg cgctcgagtt gcgctcatcg
      481 cgcttggcgt cgcgaccttc ctgttcctgt ttgtgttccc gacccgttcg tacctcgatc
      541 agcggggccg ggtcggtgcg gcccagcacg atgtgaacgt gttgcgcgaa cagaacgaca
      601 agctgcaagc cgaagcgctg caactccaga cgccggctgc gatcgagcgc atggcgcgag
      661 aacagttcca catggtgaag cccggcgagc aggtctacaa cgtgtcgcct gctcccacga
      721 cgaccagcac cacgaccccc gctccctaat atccccctcg tgccgacgca cgagcacgac
      781 gtcgcctcgt tgacccaact tctgggccgg ccgccgcgcg ccgaattcga ggtcgttgtc
      841 cgctccgacg ccggcgagcc ggccgtgatt cggaacgcgc cgttgctcga cgacggaacg
      901 ccgatgccga cgcgcttctg gctcgtcgat ccggcgctct cgctgcgtgt cgcgcgcctt
      961 gaatcggacg gcggcgttcg ggccgcggaa gcggcggtgg atgccggcgc gctgcaagcc
     1021 gcgcacgacg cctacgcgag cgaaagagac gcggcgatcg cgcccgatca cgccggtccg
     1081 aggccgcacg gcggagtcgg cggcacgcgg cgcggggtga agtgtttgca cgcgcactac
     1141 gcatactttc tcgcgggtgg ggacgatccg gtcgggcgct gggtagccca acagttggcg
     1201 gaggagcagc tggcatgagg cgcgtcgcgg caatcgacat cggaacgaac tcgacccggc
     1261 tgctcgtggc cgacgtcgac ggcaccggtc gcgacgcgaa gctcgtcccc gtcgaccggc
     1321 gcacgcagat cacgcgcctc ggtcaaggag tgcacgacgc gcgccggctg catccggacg
     1381 caatcgaacg cactctcgcg gtgctgcgcg actaccgcgt cgtgctcgac gacctcggcg
     1441 tcgaagcagt gcgcgcgacg gcaacgagcg cgtcacgcga ctcgtcgaac cgcgacgaat
     1501 tctttcagcc ggcgacgcag atcatcggag tcgagcccga gctgctgacg ggcgaagaag
     1561 aagcccgtct cgaatacctc ggcgcgaccg cctcgcttgc ggctcccgag ccgtacctgt
     1621 tgttcgatgt cggcggcggc tcgaccgagt tcatcgcggg ccgggctgaa ccggaggcgc
     1681 tcacgtcgat cgacattggt tgcgtacggc tgaccgagca gtacttgcat tccgacccgc
     1741 ccgcggccga ggagctgagc gccgcccact ccgtggtccg cgactacctc gccgacgtcg
     1801 accgtctggt ccccggcgcg gccggcgccg ccacgctgat cgggacggcg ggcaccgtct
     1861 ggaccatggc tgcggtcgag ctcggagtag atgctgcgcg cagcgacctc atcgaccatt
     1921 tccgcctgac ccgggaagct gccgaggacg tgttccggac tctggccacg gagccgatcg
     1981 cccgccgccg ccacaatccg ggcctcgagc cgggccgggt cgacgtcatc gtcggcgggg
     2041 ccatcgtcgt cgtgagcgtg ctccgccact ggggattcaa cgaattgctc gtgtcggaag
     2101 cagacatcct cgacgggctg gcccgcacga tctagcccgc gagcgcctcc tcggctaggc
     2161 gcagatccgg aggcccggac agtcgccggg actccaacgg tgttcgaggg ccggaggtcg
     2221 cagccgcagg ttcgcgtgac gttcgtaacg cgcgctttat tggcccccag gcctgccgat
     2281 gtttgacagg tggaactgtt ggccttcgag cgggcgcttg ccgatctttc ggtgccggag
     2341 atccggctga tcgcgctcga cctcgtttcg gccaacgaat gcaccgccga cgaagtcgct
     2401 cggactcgtt cggtgctcgt gatcgaacag acactgcgcc gcacgcaccg gctgcacagt
     2461 gccgcctcgg ctgcgctcga agcggcgacg accgtgcaag aagttgccga gcggggcaag
     2521 gtctcgctgc ccgacgacga cgtgacgcgt gtcgctcgtg cggccgcgca acttgcacgg
     2581 ggcttgatca tcggcgaagc gcccggcgtc gacgacgccc ttcgcgtcct gggccgcggc
     2641 tggtaccgcc tcccttgcct cgacgaactt cgcgtcgcct gacgaacatg cgtgagaaac
     2701 ggccctgagg gggcccgttt ctcacgcaag tacgatgcgc agccgtggcg cacgcggaca
     2761 ttttgctcat ggggccgggg ccgtcgaatc cgtacccaga agtcatggaa gcctttcaac
     2821 ggccggtgct cgggcacctc gatcccgatt ttctcgtcgt tctcgatgag atcggcgacc
     2881 gcctgcgtgc agtgttccgc acacaaaacg aactgacgct gccgatcagc ggtacgggtt
     2941 cggccggtat ggaagcctgc ttcgtcaacc tcgtcgagcc cgacgacacc gtcatcagtg
     3001 gtgtcaacgg cgtgttcggc ga
//
LOCUS       DASUGA010000530         4358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2035237, whole genome shotgun sequence.
ACCESSION   DASUGA010000530 DASUGA010000000
VERSION     DASUGA010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2035237"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VFX21_10480"
     CDS             <1..610
                     /locus_tag="VFX21_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX5096433.1"
                     /translation="VHHLVLTDGSKGTWDADADTVALAARREHEQTEAARRLSGERMG
                     SVSFLRYIDGELPVTREALSRVVRVIREVRPDVVLGHDPWKRYRLHPDHRNAGTLACD
                     AVIAARDPHFFRDLELPHHRPRDLLLWEADEVNHVEDVTGWVDRKLDALEAHCSQFES
                     TMKAVDEQQLAAFRTRVTDRLAGLGAPYLLGAAELFARVTDL"
     gene            complement(699..974)
                     /locus_tag="VFX21_10485"
     CDS             complement(699..974)
                     /locus_tag="VFX21_10485"
                     /inference="COORDINATES: protein motif:HMM:NF019028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1-like repetitive region-containing
                     protein"
                     /protein_id="HEX5096434.1"
                     /translation="MAAKKAAAKKAPAKKAAAKKSTAKKSTAKKAPAKKAAAKKSTAK
                     KSTAKKAPAKKAAAKKAPAKKAAAKKSTAKKSTAKKSTAKKSTAKKS"
     gene            complement(984..1265)
                     /locus_tag="VFX21_10490"
     CDS             complement(984..1265)
                     /locus_tag="VFX21_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX5096435.1"
                     /translation="MTKAELIDAVADAAGVSKADAERTIGAFFDTVVSSTKKGDKVAW
                     PGFGSFSTTKRPARTGRNPQTGAPVKIAASTAMKFTASSTLKSTLNGKK"
     gene            complement(1444..1866)
                     /locus_tag="VFX21_10495"
     CDS             complement(1444..1866)
                     /locus_tag="VFX21_10495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018821492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX5096436.1"
                     /translation="MPAGCVFCDVVAGRVAAFEVLRDDVAVAFLDKSPLFPGHTLVVP
                     TRHVVTLADLEQEEIGPYFARVRAIAAAMPEALNAGGTFVANNNVVSQSVPHLHVHVV
                     PRTRGDGLRGFFWPRRRYADGEADAYADRLRRALSDAV"
     gene            2047..3459
                     /locus_tag="VFX21_10500"
     CDS             2047..3459
                     /locus_tag="VFX21_10500"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX5096437.1"
                     /translation="MTDLISGDGARSDITNAGLPDAGIVSGASSRTRCVVDTSVLIAD
                     PHCLTSFGDAALVIPLTVVEELDSLKTRPDDVGRSARTALRAIEDLRVQHGGSLAEPV
                     AVGTGTVQIEINGVQKHLLVEHGLDPSVPDNRIIGAALGQTGIGPTVMVSNDAALRIK
                     AAHLGVGAAEHQPTKRLRSDRPMGWCVLTTSYEVVDCLYAAGAVDVAAVLGSIDGHDV
                     TLTENEFAVLRAGSQSALTRRIGDELVLLPHAVPEPWGLRPRNKEQRFALELLLDPDI
                     AVVALDGRAGTGKTILAIAAALEQVVEQPRYERVAVYRPLVPVGRADVGFLPGGLDEK
                     LDPWMSAIHDAIVALTDRGSSRDARRLIDELTARGQLSLESVTFLRGRSLQRQIVVID
                     EAQNLEPTTLRTILTRVGDGTKVVFTGDTSQIDAPYLGESNNALAVLTNAFTDQPCFG
                     HVTLTACERSDVASLAAELL"
     gene            complement(3482..>4358)
                     /locus_tag="VFX21_10505"
     CDS             complement(3482..>4358)
                     /locus_tag="VFX21_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096438.1"
                     /translation="VMAYDVNDPYRVEARDRHVRRDLAPWIVAGGIVVVAGSFAAMQW
                     SARRQRSLAADESTAFSMLGTIHHSRLSVVPRLSLYALDSVAGDRPVCTIRLADIRVD
                     GPGDVCFPVDVKGIPRPTGLVVPRVGDTVLWPRGRALVGARHQRPGRVGTPEIREAGN
                     VRKFLTWLAVCGACGLLLTATVAVVSTRGARITEKWVADGRRAVATIVSRNERSVNVD
                     VVVDDSTRPAVLMAAPVDYPEDYDPGQRYPAVVGDGDRVRLLAEPYDAVEPILWAAIP
                     SAVLLCWAARRFIGA"
BASE COUNT          678 a         1543 c         1400 g          737 t
ORIGIN      
        1 cgttcaccac ctcgtgctga ccgacggttc gaagggcacg tgggacgccg acgccgacac
       61 cgtcgcactg gccgcgagac gtgagcacga gcagaccgaa gcagctcggc gattgtcggg
      121 cgaacggatg ggatcggtgt cgttcctccg ctacatcgac ggtgaactgc ccgtcacgcg
      181 cgaggcgctc agccgggtgg tgcgagtgat ccgtgaggtt cgacccgacg tcgtgctcgg
      241 ccacgacccg tggaagcggt atcgcctgca ccccgaccat cgcaacgccg gcaccctcgc
      301 ctgcgacgcc gtgatcgccg cccgcgaccc gcacttcttc cgcgacctcg agttgccgca
      361 ccaccgccct cgcgacctcc tgctgtggga ggccgacgag gtcaaccacg tcgaggacgt
      421 caccggctgg gtcgaccgca agctcgacgc gctcgaggcg cattgcagtc agttcgagtc
      481 gacgatgaag gcggtcgacg agcagcagct cgccgccttc cgaactcgag tcaccgaccg
      541 gctcgccggc ctgggtgccc cgtaccttct cggcgccgcc gaactgttcg cccgcgtgac
      601 agatctctga tcagctggtg tcagctgcgc ggccttgccg tagacgcgaa gcgagggcag
      661 ggtggcggcc ctgccctcgt tgcgttggat ccagtggatc agctcttctt ggcagtcgac
      721 ttcttggcgg tcgacttctt ggcggtcgac ttcttcgccg tcgacttctt ggccgcagcc
      781 ttcttggcgg gggccttctt cgctgcagcc ttcttggccg gagccttctt cgcagtcgac
      841 ttcttggccg tcgacttctt cgctgcagcc ttcttggccg gagccttctt ggcagtcgac
      901 ttcttggccg tcgacttctt cgcagcagcc ttcttggccg gagccttctt cgcagcagcc
      961 ttctttgcag ccattggtgt ttcctacttc ttcccgttga gtgtggactt cagggtggag
     1021 ctggccgtga acttcatcgc ggtcgaggcc gcgatcttca cgggtgcgcc ggtctgcggg
     1081 ttgcggccgg tgcgggcagg gcgcttggtg gtgctgaacg agccgaagcc cggccacgcg
     1141 accttgtcac ccttcttggt cgaggagacc actgtgtcga agaaggcacc gatcgtgcgc
     1201 tctgcatccg ccttggacac gcctgcggcg tcagcgactg cgtcgatgag ttcagccttg
     1261 gtcatttgtt ggtttccttt ggatcacgac cggagtcgtg ctgtgtgttg gatgttgttg
     1321 tttctcgttc gagtgcacgg caaaccgtga ctctgcgctt gcgcgcagga ctcatcaaac
     1381 ttgcgcgaac tacatagatg caacgaaagc aatcgttgca tttgcaactg gattcaaatg
     1441 gcgtcagacc gcgtccgaaa gtgcgcgccg caatcgatcg gcatacgcgt ccgcttcgcc
     1501 gtcggcgtat cgccggcgcg gccagaagaa cccgcgcaag ccgtcaccgc gagtgcgcgg
     1561 cacgacgtgt acgtgaagat gcggaacgct ctggctcacg acgttgttgt tggccacgaa
     1621 ggtgccgccc gcgttcagcg cctcgggcat cgccgccgcg atcgcgcgca cgcgtgcgaa
     1681 gtacggaccg atctcctctt gctccagatc ggcgagcgtc accacgtgac gcgtcgggac
     1741 caccagcgtg tgcccgggga acagcggcga cttgtcgagg aacgccacgg cgacgtcgtc
     1801 gcgcagcacc tcgaaggcgg ccacacgtcc ggccacgaca tcgcagaaca cgcatccggc
     1861 cggcacacgc ccgacgttac gtgcgccacc caccctgtcc tgcagcgtgc cccgcaccat
     1921 gtcgcgagcc atcttgtgct gccatccacc acgacgatcc ggtcctgcgg tacaactatc
     1981 cgcgttgtcc gttccgtcct ccgccagcca ggtgatcctg gtcgatcgca gacgtcagag
     2041 gtgaccgtga ccgatctcat ttcaggtgat ggcgcgcgca gcgacatcac caatgccggc
     2101 ctcccggacg ccggcatcgt cagcggcgcg agcagccgca cacgttgcgt cgtcgacacc
     2161 tcggtgttga tcgccgaccc tcattgcctc acttcgttcg gcgacgccgc gctggtgatc
     2221 ccgttgacgg tggtcgagga gctggacagc ctcaagaccc gccctgacga cgtcgggcgt
     2281 tcggcgcgca ccgcgctgcg ggcgatcgaa gacctccgcg tccagcacgg cggctcgctc
     2341 gccgagccgg tagcggtcgg caccggcacg gtgcagatcg agatcaacgg cgtgcagaag
     2401 catctgctgg tcgagcacgg gctcgacccg agcgtccccg acaatcggat catcggtgcc
     2461 gccctcgggc agacgggcat cggcccgacc gtgatggtgt cgaacgacgc cgcgttgcgc
     2521 atcaaggccg cccacctcgg cgtcggcgcc gccgaacacc agccgaccaa gcgcctccgg
     2581 tccgatcgcc cgatgggatg gtgcgtgctc accacttcgt atgaggtcgt cgattgcctg
     2641 tatgccgccg gcgcggtcga cgtcgccgcg gtgctcggca gcatcgacgg tcacgatgtc
     2701 acgctcaccg agaacgagtt cgccgtgctg cgcgccggct ctcagtccgc gctcacgcgc
     2761 cggatcggcg acgagctcgt gctgttgccg cacgccgtac cggaaccgtg gggattgcgg
     2821 ccgcgcaaca aggagcagcg cttcgcgctc gagctgttgc tcgaccccga catcgcggtg
     2881 gtggcgctcg acggtcgggc cggcaccggc aagacgatcc tggccatcgc cgccgcgctc
     2941 gaacaggtcg tcgaacagcc ccggtacgag cgggtcgccg tgtaccgccc gctggtgccg
     3001 gtgggtcgcg ccgacgtcgg cttcctgccg ggtggcctcg acgagaagct cgacccgtgg
     3061 atgtcggcga tccacgacgc gatcgtcgcc ctcaccgatc gcggcagcag ccgcgacgcc
     3121 cgccgtttga tcgacgagct gacggcccgc ggtcagctgt cactcgaatc ggtgacgttc
     3181 ctgcggggtc gttcgttgca gcgccagatc gtcgtcatcg acgaggcaca gaacctggag
     3241 ccgacgacgt tgcgcacgat cctcacccgt gtgggcgacg gcaccaaggt cgtgttcacc
     3301 ggcgacacca gtcagatcga cgcgccgtat ctcggcgagt cgaacaacgc gctcgcggtg
     3361 ctgaccaacg cgttcaccga tcaaccctgc ttcggccacg tgacgctcac cgcctgcgag
     3421 cgcagcgacg tcgccagcct cgccgccgag ttgctgtaac ccgctcgggc gcggcacccc
     3481 gctacgcgcc gatgaaacgg cgcgccgccc aacacaacag cacggcgctc gggatcgccg
     3541 cccacaggat gggttcgaca gcgtcatagg gctcggccag caggcgcacg cggtcgccgt
     3601 cgcccacgac cgccggatac ctctggccgg ggtcgtagtc ctccgggtaa tcgaccggcg
     3661 cagccatcag taccgccggt cgtgtgctgt cgtcgacgac gacatcgacg ttgaccgagc
     3721 gttcgttgcg gctcacgatc gtcgccaccg cgcgacgccc gtcggcgacc cacttctccg
     3781 tgatccgcgc accccgcgtg ctcacgacgg cgacggtggc cgtgagcagc agaccgcatg
     3841 ccccgcacac cgccaaccac gtgaggaact tgcggacgtt gccggcctcc cgaatctccg
     3901 gtgtgccgac ccgccccgga cgctgatgcc gcgcaccgac cagcgcccgg ccgcgtggcc
     3961 acagcaccgt gtcgccaacg cgcggcacca cgagacccgt cggccgcggg atgcccttga
     4021 cgtcgacggg gaagcacaca tcgcctggtc cgtcgacacg gatgtccgcc aggcggatcg
     4081 tgcacaccgg gcgatcacct gccaccgagt cgagcgcgta caacgagagc cggggcacga
     4141 ccgacagccg gctgtggtga atcgttccga gcatgctgaa cgccgtcgat tcgtcggcgg
     4201 cgagcgagcg ctgacgccgc gccgaccact gcatcgcggc gaacgagccg gcgaccacca
     4261 cgatgccgcc ggcgacgatc cacggcgcca gatcacgccg cacgtgacgg tcccgcgcct
     4321 cgacgcgata cgggtcgttc acgtcgtagg ccatcacg
//
LOCUS       DASUGA010000531         3784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1703680, whole genome shotgun sequence.
ACCESSION   DASUGA010000531 DASUGA010000000
VERSION     DASUGA010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703680"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="VFX21_10510"
     CDS             complement(<1..1230)
                     /locus_tag="VFX21_10510"
                     /inference="COORDINATES: protein motif:HMM:NF005907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HEX5096439.1"
                     /translation="MPALFPPAAVLPGFEPAPAAPPPPPRQRSLDDLGTPLHEVTFVV
                     IDLETTGASPRDEAITEIGALKLRGGELLRRIDTLVNPGVPIPPMITVLTGITEAMVA
                     PAPRIDDLLPTIVEFIGDSVIVGHNIRFDISFLDAALVAAGYPRLTNRRVDTVGLARR
                     LVRDEVPNLRLQTLARHFRTAVEPVHRAYADAAATAEVFHTMLERAATFGVLGLDDLL
                     ELPKIRAHPSASKLALTARLPRAPGVYLFRDRAGRVLYVGKASNLRSRVRSYFSGDDR
                     RKVPQLLRETVRIDHRVCVGPLEAAVRELRLIQSLQPRFNRQAKLWRKYAYLKLTNER
                     FPRLTVTKTVRAGDVALGPFNSTNAAHLVREAIESVVPLRRCTARISAKTTPVAGAPC
                     VPAQLGVASCPCRAQVDD"
     gene            complement(1279..2646)
                     /locus_tag="VFX21_10515"
     CDS             complement(1279..2646)
                     /locus_tag="VFX21_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096440.1"
                     /translation="MGRRRRWFLLAGAVVLLAGCGGEYRWSKSFGGGSFVDTIRVGDV
                     AVAPDGAAIVVGRFEGRVDIGGTSLTANGKADIFVARYRANGDVDWVQKFGGSVSDSD
                     YANAVVFDADGNAYVGGEFRNTATFGTVTLTSASPNSDGFVAKVTPTGTVAWARAIGG
                     TGIGTDTVSALATNGTRVAATGQWTDAKGDVLLAAMNTSNGADVFAPRHEGGSEDDYG
                     NGIAIDAAGTITVTGFFKERANLAGQRLTSAGFDDTFLARYGAGGNGLTAKRFGSTGL
                     DRPASLASAGDELVLAGNFAGTVDFGGKSVTALGPGDMFVTRFGANLAPIWAQRYGGN
                     TGSAETLHDVAVDTKGNAVATGEVIASVDFGTGALNPVGTGQDPFLVKIDPTGKTVWA
                     KRFTTTSQATNVGAAVATAPDNDVVAAGAFMADITIGGTTPHTSVGGNGFTQSPDGYI
                     ARFAA"
     gene            2782..3027
                     /locus_tag="VFX21_10520"
     CDS             2782..3027
                     /locus_tag="VFX21_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096441.1"
                     /translation="MEQRYRCNGCGNLTRFDVVAVRRTRAFHHYTIGGDLEIAEEEVL
                     EEQVESVTCRWCGAAGAAIETLPADALGAEQLHDQAT"
     gene            complement(2992..3411)
                     /locus_tag="VFX21_10525"
     CDS             complement(2992..3411)
                     /locus_tag="VFX21_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012899484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX5096442.1"
                     /translation="MNARHETFTLDTKGDGEIIDVTGHAQKTIDNAGFTDGICTVFVA
                     HSTCGVTTIEFEPGCNADLNRVLEGVAPQDDAYDHNERNADTNGHSHVRAAVIGPSVT
                     VPFTNGELLVGTWQKIVCIDFDDRPRSRRLVVQLLGA"
     gene            3472..>3784
                     /locus_tag="VFX21_10530"
     CDS             3472..>3784
                     /locus_tag="VFX21_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096443.1"
                     /translation="MPDALLGPLLEVAGDTLRALDADDVPLALRHLRGFDRRGLMHGP
                     APRQLRKALDTDGAFRTAVLERFAARPEVEAEAGRWSADEAVARVEDAAGRHDLALLA
                     SM"
BASE COUNT          562 a         1258 c         1345 g          619 t
ORIGIN      
        1 gtcgtcgacc tgcgcgcgac acgggcagct cgcgacaccg agctgcgcgg gaacacacgg
       61 cgcgccggcc acgggcgtcg tcttcgcgct gatgcgcgcg gtgcaacgcc gcagcggcac
      121 gacgctctcg attgcctcac gcacgaggtg cgcggcgttc gtcgagttga acggtccgag
      181 cgcgacgtcg cctgcgcgca ccgtcttcgt caccgtcaag cgcgggaatc gttcgttggt
      241 cagcttcaag tacgcgtact tgcgccagag cttggcctga cggttgaagc gcggctggag
      301 cgactggatc aaccgcagct cgcgcaccgc ggcctcgagt gggccgacac acacgcggtg
      361 gtcgatccgg acggtttctc gcagcaactg gggcaccttg cggcggtcgt cgcccgagaa
      421 gtacgagcgc acccgcgacc gcaggttcga tgccttgccg acgtacagca cgcgtcccgc
      481 tcggtcgcgg aagaggtaga cgccgggcgc gcggggcagg cgcgccgtca acgcgagctt
      541 cgacgccgac ggatgcgcac ggatcttcgg cagttcgagc aggtcgtcca gcccgagcac
      601 accgaaggtc gcggcgcgtt cgagcatggt gtggaacacc tcggctgtcg cggcggcgtc
      661 ggcgtaggcg cggtgcacgg gttcgaccgc ggtacggaag tggcgggcga gtgtttgcag
      721 gcggaggttc ggtacttcgt cgcgcacgag ccgccgcgcc aacccgaccg tgtcgacgcg
      781 ccggttggtc aggcgcgggt agcccgcggc gacgagcgcg gcgtcgagga agctgatgtc
      841 gaaacggatg ttgtgaccga cgatcacgct gtcgccgatg aactcgacga tcgtcggcaa
      901 caggtcgtcg atgcgcgggg cgggcgccac catcgcttcg gtgatgccgg tgagcacggt
      961 gatcatcggc gggatcggca cgccgggatt cacgagcgtg tcgatgcgcc ggagcagctc
     1021 gcccccgcgc agcttcagcg cgccgatctc ggtgatcgcc tcgtcgcggg gcgacgcgcc
     1081 ggtggtctcg aggtcgatga cgacgaacgt cacctcgtgc agcggggtgc cgagatcgtc
     1141 gagcgagcgc tggcgcggcg gtggtggcgg cgcggccggc gcgggctcga agcccggcag
     1201 cacggcggca ggggggaaga gggccggcat gcgcggccca ggatagcgaa catatgttcg
     1261 cgacgaaagt atgttcgatt acgctgcgaa ccgcgcgatg tagccgtccg gcgactgggt
     1321 gaagccgttc ccgccgacgc tcgtgtgggg cgtcgtcccg ccgatcgtga tgtcggccat
     1381 gaacgcgccg gccgcaacga cgtcgttgtc gggcgctgtc gcgactgccg cgccgacgtt
     1441 cgtggcctgg ctcgtcgttg tgaagcgctt cgcccacacg gttttgccag tcgggtcgat
     1501 cttcacgagg aacggatcct ggccggtgcc gaccgggttc aaggcgccgg taccgaagtc
     1561 gacggaggcg atgacctcgc ccgtcgctac ggcgttgccc ttggtgtcca cggcgacgtc
     1621 gtgcagcgtc tcggcgctgc cggtgttgcc gccgtatcgt tgtgcccaga tcggcgccag
     1681 gttggcgccg aaacgtgtga cgaacatgtc gccgggaccg agcgcggtca cactcttgcc
     1741 gccgaagtcg acggttccgg cgaagttgcc ggcgaggacg agttcgtcgc ccgccgacgc
     1801 gagggatgcg ggccggtcga ggccggtcga accgaatcgc ttcgcggtga ggccattacc
     1861 acccgcgccg tagcgcgcga ggaacgtgtc gtcgaagccc gcactcgtca atctctggcc
     1921 cgcgagattg gcgcgttctt tgaagaaacc tgtcacggtg atggtgcccg cggcatcgat
     1981 cgcgatgccg ttgccgtagt cgtcttcgct cccgccttcg tgacgcggtg cgaacacgtc
     2041 ggcgccattc gaggtgttca tcgcggcgag cagcacgtcg cccttcgcat cggtccactg
     2101 tcctgttgcg gcgacgcgcg tgccgttggt cgcgagggcg ctcaccgtgt cagttccgat
     2161 cccggtgccg ccaatggccc ttgcccacgc gaccgtcccg gtcggtgtga ccttcgcgac
     2221 gaagccatcg gaattcggtg acgccgaggt gagtgtcacc gtcccgaacg tcgctgtgtt
     2281 cctgaactcg ccgccgacgt acgcgttgcc gtcggcatcg aacacaacgg cgttggcgta
     2341 atcgctgtcg ctcacggagc caccgaactt ctgcacccag tcgacgtcgc cgttcgcgcg
     2401 gtagcgcgcg acgaagatgt cggccttgcc gttcgcggtc agcgaagtgc caccgatgtc
     2461 gacgcgcccc tcgaagcggc cgaccacgat cgcggcgccg tcgggagcaa ccgcgacgtc
     2521 gccgaccctg atcgtgtcga cgaagctgcc gccgccgaac gatttcgacc accggtactc
     2581 gccgccgcag cccgcgagca gcacgactgc gcctgcgagc aagaaccacc gacgtcgccg
     2641 tcccatgacg tcccccctgg tgtgcccatc ccttggcact ggatcgcaat gtaccggtca
     2701 ccggtgaaat cggcaatatc gcccggaaac tgaaggaagc ggcgaaaccg gacacgccgt
     2761 ctaccgccta acctccgggc gatggagcag cgctaccggt gcaacgggtg cgggaatctc
     2821 acccgcttcg acgtggtcgc ggtccggcgc acccgggcgt tccaccacta cacgatcggc
     2881 ggtgacctcg agatcgccga agaagaggtg ttggaggagc aggtcgagtc cgtgacctgc
     2941 cggtggtgcg gcgccgcggg cgccgcgatc gagaccctgc cggccgacgc tctaggcgcc
     3001 gagcaactgc acgaccaggc gacgtgaacg gggccggtcg tcgaagtcga tgcagacgat
     3061 cttctgccac gtcccgacga ggagctcccc gttcgtgaac ggcacggtca ccgacgggcc
     3121 gatcacggct gcgcgcacgt gtgagtggcc gttggtgtcg gcgttgcgct cgttgtggtc
     3181 gtacgcatcg tcttgcggcg cgacgccctc gagcacacgg ttcaagtcgg cgttgcagcc
     3241 gggctcgaac tcgatggtcg tcaccccgca ggtcgagtgg gcgacgaaca cggtgcagat
     3301 cccgtcggtg aagccggcgt tgtcgatcgt cttctgtgcg tggccggtga cgtcgatgat
     3361 ctccccgtcg cccttcgtgt ccagcgtgaa agtctcgtgt cgggcgttca tcggttcctc
     3421 cgcggcagct gagcccgggc agtctacgga atgacgttgc cggcggcctc tgtgcccgac
     3481 gcattgctcg ggcccctcct cgaagtggcc ggcgacaccc tacgagcgct ggatgccgac
     3541 gacgtgccgt tggcattacg gcacctgcgc ggcttcgacc gtcgcgggct gatgcacggc
     3601 cccgcgcccc gccagctgcg gaaggcgctc gacaccgacg gtgcattccg gacggcggtg
     3661 ctcgagcggt tcgcggcgcg gccggaggtg gaggccgagg ccggtcgctg gtccgcggac
     3721 gaggcggtcg cgcgtgtcga ggacgcggcc ggccgtcacg atctcgcgtt gctcgcgtcg
     3781 atgc
//
LOCUS       DASUGA010000532         3310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1044942, whole genome shotgun sequence.
ACCESSION   DASUGA010000532 DASUGA010000000
VERSION     DASUGA010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044942"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..368)
                     /locus_tag="VFX21_10535"
     CDS             complement(3..368)
                     /locus_tag="VFX21_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096444.1"
                     /translation="MPNVDPEVEAMLEFSERPRTNDWSLRAALVRYAQAQPVRTESIL
                     DLVRRVEWALNKKPDAGVLAVAHELDGLGDVLAEWAVEGVLPGPDDEVDRVVAHVAAR
                     LEELGVPKQERPPGPRNRG"
     gene            complement(370..1107)
                     /gene="merB"
                     /locus_tag="VFX21_10540"
     CDS             complement(370..1107)
                     /gene="merB"
                     /locus_tag="VFX21_10540"
                     /EC_number="4.99.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF015216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018836 - alkylmercury lyase activity
                     [Evidence IEA];
                     GO_process: GO:0046413 - organomercury catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial lyase"
                     /protein_id="HEX5096445.1"
                     /translation="MPHNVPGFEFSDDAKRCRAFIFDFWCAHGRGPTLREVNEETGFD
                     RRRILEAYKELQLGIVITVQEDTQNGNLLKAPPFSSFPSQVRAYIDDKFHSFVGCASE
                     AVAFSHMPPFKDKTVRLESHCACCLEPVTLVSKNFELQSVTPATTMLHISLSPYDWNN
                     VDMLRMCDSMNYVIDADHADRYERQTSSRGILVNFDQAKMFVQGVANERMWNHDWKPG
                     VLNPKAVIKGFRMMGVDTSPWEVPRGG"
     gene            complement(1130..1789)
                     /locus_tag="VFX21_10545"
     CDS             complement(1130..1789)
                     /locus_tag="VFX21_10545"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX5096446.1"
                     /translation="MPSDACRDFRGALAAMALGNIDDTEAIALRAHLDGCAECRADLR
                     DLEAVSAVLPLAELDRVTEEPELPSQLGRQVLQRITTERRRRRGLRTRRALAIAAAML
                     VVLGIGAALVVNRSDSGGGTEVVFPTVEGVHGQAVLEAHDAGTEVELDAGGLRTGERY
                     WLWLTGDDGKRVAAGTFTGQPDDIHVTLTAGLPLRDTRRIWVTDEDDAVVLDAPLASA
                     Q"
     gene            complement(1779..2303)
                     /locus_tag="VFX21_10550"
     CDS             complement(1779..2303)
                     /locus_tag="VFX21_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020557735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5096447.1"
                     /translation="MSVLGTNEGVRAVYEAHGAELYRFALRSLGDRGLAEEAVQETFV
                     RAWQAAGRFNDSLGSLRTWLFAILRNVVIDSSRARAIRPGLADGDGAGADVDLTDDAF
                     DRMLTAWQVEEALRMLSDEHRSALVEVHYLGRTYADVAEERGVPVGTIKSRVFYALKA
                     MRLALEELGWPDAL"
     gene            2478..3101
                     /locus_tag="VFX21_10555"
     CDS             2478..3101
                     /locus_tag="VFX21_10555"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096448.1"
                     /translation="MAGDTPRELNMRRTWRVMAVGAVLALVLSACGGDDDDDVSAADN
                     PTTTARSSETTSATTPVAGSDEGGQQSDVTVAYGETALGDTLVDGSGMTLYVFDADTS
                     ATSTCVDACAENWPPLIVSGQPVVGDGLDDSLFAVSTRPDGAQQLTVDGHPLYLFKGD
                     TKAGDTNGQGVLDKWYAAGIDGSKVGDTDASGETETSAKPSSSGDGY"
     gene            complement(3126..>3310)
                     /locus_tag="VFX21_10560"
     CDS             complement(3126..>3310)
                     /locus_tag="VFX21_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006381290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX5096449.1"
                     /translation="RVMASALQVHGGIGFTWEHDLHFFLKRAQLDQVMFGDASYHRAR
                     LTSLLRPRVEAGESVV"
BASE COUNT          548 a         1141 c         1096 g          525 t
ORIGIN      
        1 attcagccgc gatttcgggg gccgggtggc cgttcctgct tcgggacgcc gagttcctcg
       61 aggcgcgccg cgacgtgcgc gacgacgcgg tcgacctcgt cgtcggggcc ggggagcaca
      121 ccctcgaccg cccactccgc gagtacgtca cccaggccgt ccagctcgtg cgcgacggcg
      181 agcacgcccg cgtccggctt cttgttcagc gcccactcga cgcggcgcac gaggtcgagg
      241 atcgactccg tgcggaccgg ttgcgcctgc gcgtagcgga cgagcgcggc ccgcaggctc
      301 cagtcgttcg tgcgcggccg ctcggagaac tcgagcatcg cttcgacttc ggggtcgacg
      361 ttcggcacgt cagcccccgc gcggcacttc ccacggcgac gtgtcgacgc ccatcatgcg
      421 gaagcccttg atgaccgcct tcgggttgag cacgcccggc ttccagtcgt ggttccacat
      481 gcgttcgttg gcgacacctt gcacgaacat cttcgcctgg tcgaagttga cgaggatgcc
      541 gcgcgagctc gtctgccgct cgtagcggtc ggcgtggtcc gcgtcgatga cgtagttcat
      601 agagtcgcac atgcggagca tgtcgacgtt gttccagtcg tacggcgaca gcgagatgtg
      661 cagcatcgtc gtcgcgggcg tcaccgactg gagctcgaag ttcttggaga cgagcgtcac
      721 cggttcgagg cagcaggcgc agtgcgactc gagccgtacc gtcttgtcct tgaacggcgg
      781 catgtgcgag aacgcgaccg cctcgctcgc gcagccgacg aacgagtgga acttgtcgtc
      841 gatgtaagcg cgcacttgcg acgggaacga cgagaacggc ggcgccttga gcaagttgcc
      901 gttctgcgtg tcttcctgca ccgtgatcac gatgccgagc tgcagctcct tgtaggcctc
      961 gaggatgcgt cgccggtcga agccggtctc ctcgttgacc tcgcgcagcg tcggcccgcg
     1021 gccgtgtgca caccagaagt cgaagatgaa cgcgcggcag cgcttcgcgt cgtcggagaa
     1081 ctcgaacccg ggaacgttgt gcggcatgcc cggagactac aaaacgcgcc tactgcgccg
     1141 acgcgagcgg tgcatccaag acgacggcgt cgtcctcgtc ggtcacccag atgcggcgcg
     1201 tgtcgcgcaa cggcagcccg gccgtgagcg tcacgtgaat gtcatcgggt tggccggtga
     1261 acgtgcccgc cgcgacccgt ttgccgtcgt cgccggtgag ccacagccaa tagcgctcgc
     1321 ccgtgcgcag tccgccggcg tcgagctcga cctcggtgcc cgcgtcgtgt gcttcgagca
     1381 ccgcctggcc gtgcacgcct tcgacggtcg gaaagaccac ttcggtcccg ccaccggaat
     1441 cggaccggtt gacgaccaac gcggcgccga ttcccagcac gaccaacatc gcagccgcga
     1501 tcgcaagcgc gcgccgcgtc cgcagccccc gacggcgccg gcgttcggtg gtgatccgct
     1561 ggagcacctg cctgcccagt tgcgacggca gctccggctc ctcggtcacg cgatcgagct
     1621 cggcgagcgg cagcaccgcg ctcaccgcct cgaggtcacg cagatcagca cggcattccg
     1681 cacagccgtc gagatgggcg cgcaatgcga tcgcctcggt atcgtcgatg ttgccgagtg
     1741 ccattgcggc gagcgcgccg cggaagtcgc gacacgcgtc agagggcatc cggccacccc
     1801 agctcttcga gcgcgagccg cattgccttc aatgcataga acacgcggct cttgatcgtg
     1861 ccgaccggca ccccgcgctc ctcggcgacg tcggcgtacg tacggccgag atagtgcacc
     1921 tcgacgagcg cagaccgatg ctcgtcgctc agcatccgca gcgcctcttc cacctgccaa
     1981 gcggtgagca tccggtcgaa cgcatcgtca gtgagatcga cgtcggcacc cgcaccgtct
     2041 ccgtcggcta agccgggccg gatggcgcga gcgcgcgaac tgtcgatgac gacgttgcgc
     2101 aggatcgcga acagccaggt gcgcaaactg ccgagcgagt cgttgaagcg cccggctgct
     2161 tgccaggccc ggacgaacgt ttcctgcacc gcttcttctg ccaacccgcg atccccgagc
     2221 gaacgcaacg caaatcggta caactccgcg ccgtgtgcct cgtagaccgc gcggactccc
     2281 tcgttcgtac cgagtaccga cattccacgt caggatacga gtggatggcg tgtcgggttc
     2341 atgaaccaat ccgatgcacc gtccgtttcc atcagcatgg tcttgcttgg ccgagtcctc
     2401 gtggcggctg ccttcggttt ggccgtttgg atccttcgac ggagggcgag agacactgac
     2461 gaccgcgacg aatggtcgtg gccggcgaca ctccccggga gctgaacatg cggcgcacat
     2521 ggcgggtcat ggcggtcggt gcggtgctcg cactcgtgct gagcgcgtgc ggcggcgatg
     2581 acgacgacga cgtctccgcc gccgacaacc cgacgacgac cgcccggtcg tcggagacca
     2641 cgagcgcgac gacgccggtc gcggggtcgg acgaaggcgg gcagcagagc gacgtgaccg
     2701 tcgcctacgg cgagacggca ctcggcgaca ccctcgtcga cggctcgggc atgacgctct
     2761 acgtcttcga cgccgacacc agcgccacgt cgacctgcgt cgacgcgtgc gccgagaact
     2821 ggccgccgct catcgtctcc ggacagccgg tcgtcggtga cggattggac gactcgttgt
     2881 tcgccgtgtc gacgcggccc gatggcgcgc aacaactgac cgtcgacggc cacccgctct
     2941 acctcttcaa gggcgacacc aaggccggcg acacgaacgg tcagggcgtg ctggacaagt
     3001 ggtacgccgc cgggatcgac ggttcgaagg tcggcgacac ggacgcgtcg ggcgagaccg
     3061 agacgagtgc gaagccgagc agcagcggcg acggctactg atcgacggcg tcgacggccg
     3121 gtcgttcaca caacgctttc gccggcctcg acgcgaggcc ggagcagcga tgtcaagcgc
     3181 gcgcggtgat acgacgcgtc accgaacatc acctggtcga gctgagcgcg cttcaagaag
     3241 aagtgcaagt cgtgttccca cgtgaacccg atcccaccgt ggacctgcaa cgccgacgcc
     3301 atcacgcgct
//
LOCUS       DASUGA010000533         2109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_618325, whole genome shotgun sequence.
ACCESSION   DASUGA010000533 DASUGA010000000
VERSION     DASUGA010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618325"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1389
                     /gene="ligD"
                     /locus_tag="VFX21_10565"
     CDS             <1..1389
                     /gene="ligD"
                     /locus_tag="VFX21_10565"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX5096450.1"
                     /translation="PKGPSLDPAAKHLAVHVEDHPIEYLDFEGVIPAGEYGGGDVIVW
                     DRGTWEPHDTDDPVGAVEAGELHVDLYGEKLRGRFILIRRDADRGGKEQWLLFHKRDE
                     HAVAGWDPEAFPNSVRSGRTNEEVAAHPEALWRSGAETAVANIDFSSTRADIAALDAL
                     GAKGAWDFDGVTLHLTNLDKVLFPPQRRGGRPVTKRDLIRYYAQIAPVMLPYLRDRAL
                     NMHRYPDGVGRPGFWHKEVPGHAPDWITRWHYDAADADESQHYIVADRPATLAWLANF
                     GAVELHAWTSRIDAPDCPTYALIDVDPGDKTSWEEVLTLARLYRTVLEHLAVRGVPKV
                     TGQRGVQVWVPIRTGPGFDETRAWVEAVSRAIGQTVPQLVSWKWQKRERGGLARLDYT
                     QNAINKTLVAPYSVRAAPGATVSMPIHWDELDDPELRSDRWCIGDVGTRVMSVGDVFA
                     GVLDETQALPTV"
     gene            1612..1875
                     /locus_tag="VFX21_10570"
     CDS             1612..1875
                     /locus_tag="VFX21_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX5096451.1"
                     /translation="MLADVRTYIVEELSEGDVDPAQLDELTNLIEEDILDSLGIMSLV
                     EFIEERYGLDIDPAEIEIENFETLAAITSLVAAKQAARAAHDA"
     gene            1868..>2109
                     /locus_tag="VFX21_10575"
     CDS             1868..>2109
                     /locus_tag="VFX21_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096452.1"
                     /translation="MLDVNSSDEARVAPLRILVGADPDVLRCSVEGGLTDRIAARTDG
                     EIAAVVDVLPVGRADSLLARVRADPTLFATVDVVVCS"
BASE COUNT          355 a          709 c          713 g          332 t
ORIGIN      
        1 ccgaagggcc cgtcgctcga tcccgcggca aagcacctgg cggtgcacgt cgaagaccat
       61 ccgatcgaat acctggactt cgaaggcgtc attcccgcgg gtgagtacgg cggcggcgat
      121 gtcatcgtgt gggaccgcgg cacctgggag ccgcacgaca ccgacgaccc tgtcggcgcg
      181 gtggaggcgg gcgaactgca cgtcgacctg tacggcgaga agctacgcgg ccggttcatc
      241 ctcatccggc gcgacgccga caggggtggc aaggagcaat ggctgctgtt ccacaagcgc
      301 gacgagcacg ccgtcgccgg ctgggacccc gaagcgttcc cgaactcggt gcgcagcggc
      361 cgcacgaacg aggaagtcgc cgcgcatccc gaagcgctct ggcgcagtgg tgccgagacc
      421 gccgtggcca acatcgactt ctcgtcgacg cgagccgaca tcgccgcgct cgacgcgctc
      481 ggcgcgaagg gagcgtggga cttcgacggt gtcacgttgc acctcacgaa cctcgacaag
      541 gtgctcttcc cgccgcagcg acgcggcggc cgaccggtca ccaaacgcga cctgattcgc
      601 tactacgcgc agatcgctcc ggtgatgctg ccgtacctgc gcgatcgggc gctcaacatg
      661 caccggtatc cggacggtgt cgggcggccc ggcttctggc acaaggaagt ccccgggcac
      721 gccccggact ggatcacgcg ttggcactac gacgcggccg atgccgacga gtcgcagcac
      781 tacatcgtcg ccgaccgccc ggcgacgctc gcgtggctgg ccaacttcgg ggcagtcgag
      841 ctgcacgcat ggacgtcgcg catcgatgca cccgactgcc cgacgtacgc gctgatcgac
      901 gtggatccgg gtgacaagac gtcgtgggag gaagtactca cgctggcccg gctctaccgg
      961 accgtgctcg agcatctggc cgtgcgcggc gtcccgaagg tcaccggtca gcgaggggtg
     1021 caggtgtggg tgcccatcag gaccggaccc ggtttcgacg agaccagggc ctgggtcgag
     1081 gccgtctcgc gcgcgatcgg tcagacggtg ccgcagctcg tgagttggaa gtggcaaaag
     1141 cgcgagcggg gcggcctggc ccgcctcgac tacacccaga acgcgatcaa caagacgctc
     1201 gtcgctccgt attcggtgcg cgccgcgccc ggcgcgacgg tgtcgatgcc gatccattgg
     1261 gatgagctcg acgatcccga gctgcgtagt gatcgttggt gcatcggtga cgtcggcacg
     1321 cgcgtcatgt cggttggtga tgtgttcgcg ggcgtgctcg acgaaacaca agcgttgccg
     1381 acggtctgat cgcaactgtc atggcgaaga cccgcagcgc gcggcgccgc gcgagtcagc
     1441 gatcttttcc gtcgtcgccg gaacgccgaa cgggtgcgaa tgccacgaag gcgtcgtcat
     1501 cacctgtgta cagaccgtca gtcgttttct acgatcaccg gccaggtggg cacctgagga
     1561 gtactgacgg ggtactccct gcgcgagaca ggcgtcaaga cgatgacgga cgtgctggcc
     1621 gacgttcgca cctacatcgt cgaggagttg tcggagggtg atgtcgaccc cgcgcaactc
     1681 gacgagctga ccaacctcat cgaagaagac attctcgatt cgctcggcat catgtcgctc
     1741 gtcgagttca tcgaggagcg ctacgggctc gacatcgacc cggccgagat cgagatcgag
     1801 aacttcgaga cgctcgccgc gatcacgagc ctcgttgccg cgaagcaggc cgcgcgcgcg
     1861 gcacacgatg cttgatgtca actcgtccga cgaggcgcgt gtcgcgccgc tccgcatcct
     1921 cgtcggcgcg gaccccgacg tcctgcgttg ctccgtcgag ggcggcctca ccgatcgcat
     1981 cgcggcccgc accgacggcg agatcgcggc cgttgtcgac gtgctgccgg tcggccgggc
     2041 ggactcgttg ctcgcgcgag tccgcgccga ccccaccctc ttcgcaaccg tcgacgtcgt
     2101 cgtctgctc
//
LOCUS       DASUGA010000534         2811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_20188, whole genome shotgun sequence.
ACCESSION   DASUGA010000534 DASUGA010000000
VERSION     DASUGA010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20188"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VFX21_10580"
     CDS             complement(<1..300)
                     /locus_tag="VFX21_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096453.1"
                     /translation="MRVVRAVFLAFGAFWGSWAVIALDVKDFLRFSEGQFGLLLSATV
                     LAGVFVNTIGGLLIERVGTRRMLAGVLFVWGTFMLGLTVAQSRVAFCAVFLATVAA"
     gene            370..1146
                     /locus_tag="VFX21_10585"
     CDS             370..1146
                     /locus_tag="VFX21_10585"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5096454.1"
                     /translation="MADVHDLQAAPVAVDGRAAAPVDLHREPPATPWAFFSGRRYALA
                     VVGLLFVALAASAATENGWLLLRWDRPIQQFVEDHRTAFLTTFFLFVSRFGSTMVVLG
                     LGALFTVLCWQRCRAVSYAIIVATLSRPLLEATVKEIVGRDRPAYDRLVDGTGYSFPT
                     GHVMAAVALWGLLPVIVGLFTKSRALWWASVVVSGVMIVAIGASRVYLGVHWFSDVIA
                     GLLVGTFFLLAIEQVLAHAHRTDHCARGGCGTRTDCADAT"
     gene            complement(1143..1838)
                     /locus_tag="VFX21_10590"
     CDS             complement(1143..1838)
                     /locus_tag="VFX21_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5096455.1"
                     /translation="MKCPAGHESQTTDFCDECGAPMGGPTTAPVSVTAPASEPAGANA
                     CPQCGTARTAADKFCEVCGYDFLAQQAAVAPTPTPASAAETAAPTPAMETPVRAPAGT
                     TWQIVVRADRARYDADVPDAPPFPDQYNERTFALTGDRLLIGRESQSRGIHPDIDLAG
                     QPEDTGISRSHATLRREKDGSYSIVDDGSTNGTHVNDGTDPIARGVPVPLADGDHIYI
                     GAWTVLHVRRVES"
     gene            complement(1849..>2811)
                     /locus_tag="VFX21_10595"
     CDS             complement(1849..>2811)
                     /locus_tag="VFX21_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015623111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096456.1"
                     /translation="NAETRLAAKQAVERLAPVGGTAISTWLSLANKLFQQHAGAIKHA
                     ILLTDGRNESEPEERLDRVVRECEGQFQCDCRGVGANWVVSELRDISTALLGTVDIIP
                     QPDLIDDDFRAMTERAMGKAVSSVALRVWTPKGARLRFVKQVAPQIEDLTARATVVNP
                     LTADYPTGAWGDEERDYHVCIDVPANPVGAEMLAARVSIVVDGEVASQSLVKANWTND
                     HELSTQINEEVAHYTGQAELADAIHAGLAARKAGDEQTATVKLGRAAQLAAETGNDAT
                     TRLLAKVVDIDDPATGTVRLKRNVDDVDEMALDTRSTKTVRVNK"
BASE COUNT          382 a          968 c          956 g          505 t
ORIGIN      
        1 ggccgcgacc gtcgcgagga agaccgcgca gaacgcgacg cggctctggg cgaccgtcag
       61 ccccagcatg aacgtgcccc acacgaacag caccccggcg agcatccgcc gcgtcccgac
      121 ccgttcgatc agcaggccac cgatcgtgtt gacgaacaca ccggcgagca ccgtcgccga
      181 caggaggagg ccgaattgtc cttcgctgaa acggaggaaa tccttgacgt ccagtgcgat
      241 gacggcccac gacccccaga aggcgccgaa ggccaggaac accgcacgga cgactctcat
      301 gtgaagatct tgtcgccatc agggacgcag acggcttgtg tactgacggg ccggcgccga
      361 tgaggtgtaa tggccgacgt gcatgacttg caggctgccc cggtagccgt tgacggtcgc
      421 gcggccgcac cggtcgatct ccaccgcgaa cctccggcta ccccgtgggc gttcttctcc
      481 gggcgccggt acgcgctcgc cgtcgtcggg ttgctcttcg tggcgctggc cgcgtcggcc
      541 gccaccgaga acggctggct gctgttgcgc tgggatcgcc cgatccagca gttcgtcgaa
      601 gaccaccgca cggccttcct gacgacgttc tttctcttcg tctctcgctt cgggtccacg
      661 atggtcgtcc tcggcctcgg cgcgctgttc acggtgctgt gctggcaacg gtgtcgcgcg
      721 gtgagctacg cgatcatcgt ggcgacgctg agccgtccgt tgctcgaagc cacggtcaag
      781 gagatcgtcg gccgcgaccg gccggcctac gaccgcctcg tcgacggcac tggctattcg
      841 ttccccaccg ggcacgtcat ggccgcggtc gcgctctggg gtctccttcc ggtgatcgtc
      901 gggctgttca cgaaatctcg ggcgttgtgg tgggcgtccg tcgtcgtgtc gggcgtgatg
      961 atcgtcgcaa tcggggccag ccgtgtgtac ctcggtgtgc actggttctc cgacgtcatc
     1021 gccggactcc tcgtcgggac gttcttcttg ctcgccatcg agcaggtact cgcgcacgcg
     1081 caccgcaccg atcactgcgc gcgcggcgga tgcggcacgc gcactgattg cgcagacgcg
     1141 acctagctct cgacgcgccg gacgtgcaag accgtccacg cgccgatata gatgtgatcg
     1201 ccgtcggcca gcggaacggg tacaccgcgc gcgatcgggt cggtgccgtc gttgacgtgg
     1261 gtgccgttcg tcgacccgtc gtcgacgatc gagtagctgc cgtccttctc gcgccgcaac
     1321 gtcgcgtgtg atcgagagat gccggtgtcc tcgggctgtc ccgcgagatc gatgtcggga
     1381 tgaatgccgc gcgattggct ctcgcgcccg atcagcagac ggtcgccggt gagcgcgaac
     1441 gtccgctcgt tgtactggtc gggaaacggc ggcgcatcgg ggacatcggc gtcgtagcgg
     1501 gcccggtcgg cgcgcacgac gatctgccac gtcgtgccgg ccggcgcgcg caccggcgtc
     1561 tccattgccg gcgtgggcgc ggcggtctcg gccgcgctcg cgggtgtcgg tgtcggcgcg
     1621 accgcggcct gctgggcgag gaagtcgtag ccgcacacct cgcagaactt gtcggcggcg
     1681 gtacgcgcgg tcccgcactg cgggcacgcg ttcgcaccgg ccggttcgct cgccggtgcg
     1741 gtgacgctca ccggtgcggt cgtgggccca cccatgggcg cgccgcactc gtcgcagaag
     1801 tcggtcgtct gggattcgtg acccgccggg cacttcatga catgaccttc acttgttcac
     1861 gcggacggtc ttcgtggagc gagtgtcgag cgccatctcg tcgacgtcgt cgacgttgcg
     1921 cttcaggcgg acggtgcccg tcgcggggtc gtcgatgtcg acgaccttcg cgagcagacg
     1981 cgtcgtcgcg tcgttccccg tctcggccgc gagctgcgcg gcccggccga gcttgacggt
     2041 cgccgtctgc tcgtcaccgg ccttgcgcgc cgcgagaccg gcgtggatcg catcggccag
     2101 ctccgcctgc cccgtgtagt gcgcgacctc ttcgttgatc tgcgtcgaca gctcgtggtc
     2161 gttggtccag ttggccttga ccaacgactg tgacgcgacc tcgccgtcga cgacgatgct
     2221 cacccgcgcc gccaacatct ctgcgccgac cgggttggcc ggcacgtcga tgcacacgtg
     2281 gtagtcacgc tcctcgtcgc cccatgcgcc cgtcggatag tcggcagtga gcggattcac
     2341 gaccgtcgca cgcgcggtga ggtcctcgat ctgcggtgcc acctgcttca cgaatcgcag
     2401 ccgagcgccc ttcggcgtcc acacgcgcag cgcgacgctg ctgacggctt tgcccattgc
     2461 gcgctcggtc atggcgcgga agtcgtcatc gatgaggtcg ggctgcggga tgatgtcgac
     2521 ggtcccgagg agcgcagtcg agatgtcgcg cagctcgctg acgacccagt tcgcgcccac
     2581 gccacgacag tcacattgga actggccctc gcactcgcgc acgacccggt cgagacgttc
     2641 ctcgggttcg ctctcgttgc ggccgtcggt gagcaggatc gcgtgcttga tcgcgccggc
     2701 atgttgctgg aacaacttgt tggcgagcga cagccacgtg ctgatcgcgg taccaccgac
     2761 cggcgcgagg cgttcgaccg cctgcttcgc ggcgaggcgc gtctcggcgt t
//
LOCUS       DASUGA010000535         5293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_924634, whole genome shotgun sequence.
ACCESSION   DASUGA010000535 DASUGA010000000
VERSION     DASUGA010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924634"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VFX21_10600"
     CDS             <1..1048
                     /locus_tag="VFX21_10600"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEX5096457.1"
                     /translation="ALFRHPLGVPIPHTAIITERKDQFGETLGEFVQENFLSADAISE
                     RVRASNAVDRAATWLADPVNARSVSAHAADLAVGLADLVRDEDVHTVLETEIRNAVDR
                     IELAPLAGRALKFATVQGRHQQLLDAVLIGGQLYLDENRQMLHDRFAQQAPWWLPGAV
                     EDRIFDRILDGFRDLLRSINDDPNHEFRAQFDARVADLAERLERDPEMRARGEQIKQE
                     LLSHEQLREWTASLWSDAKRTLREQAGDPDSAVRVRLAEGIAAAGARLRDDPNLAHKA
                     DELIDAGIRYVVDHFQTELAQLVSGTIARWDGEETSHRLELLLGPDLQFIRINGTVVG
                     ALVGLAIHTFAQML"
     gene            1051..1620
                     /locus_tag="VFX21_10605"
     CDS             1051..1620
                     /locus_tag="VFX21_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096458.1"
                     /translation="MRPRGPRTEDVLEPLPVIEDGVEIAPAHAARATNVSSAVRECVD
                     AVKAVLDESGQVRRQMPFTPTEPPRPPRVARFVSTFSRMIALEPRRLEALPEWWMRHQ
                     HEGRVQITRRLWFEAPRSVARGEWRARGRLRSPWLMRSIPVELVMWKHLDAWTRMMLE
                     PQRSVHVGRRYFRRGHRVLDELTRQLLST"
     gene            complement(1652..2140)
                     /locus_tag="VFX21_10610"
     CDS             complement(1652..2140)
                     /locus_tag="VFX21_10610"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5096459.1"
                     /translation="MPFPRDEIDATMQRYVAVREAIDAGQGTWADLAPFFVDDAVFID
                     PAWGRVEGIEEMKRTVFGDAMVGFEEWKFPIEFYAIDGDTIVVKWKQVLPGRKDDGRP
                     FEQSGVSTIVYAGNGKFSYEEDLLNMVHVLEDMKASKCPVPDVAMPPRHPNRDFSRPR
                     NV"
     gene            2371..3597
                     /locus_tag="VFX21_10615"
     CDS             2371..3597
                     /locus_tag="VFX21_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096460.1"
                     /translation="MTIGLLVAIVAVWAAAFTAVNRRADAVRDAMQRAEPLIVSAQGI
                     NTELARADASAAASFLVGGVEAAGPRQQYLDSIDAAMRELSVAAQNGGSVEGASDAIA
                     TISEKVPEYAGLIESARANNRQGFPIGAAYLRAASALLQDEIFPAAERLYDATVDAYA
                     DAYADAGAARDAPLLAFAGIVLLAALVATQILLFRRTNRVFNVPLVLATVVALVLVFM
                     PLTAMSHEHRELARATDEGYFGVNTIARVRILAFRARGDESFALIARGNGERWYTDFD
                     TVMSAIGSPDDDGLLGAAFEEEPIDAFSSDLADALDHYKSAHQSVQETDRAGDFDMAV
                     TLATGDAATSSATAFAALDEELKATLDEYAARFERGAGRAEKQLDNQEVWVSVLAILV
                     ALGVVVGLQQRIREYR"
     gene            3594..4589
                     /locus_tag="VFX21_10620"
     CDS             3594..4589
                     /locus_tag="VFX21_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014445435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5096461.1"
                     /translation="MRLRTFMRTGGAAVAAVMLLAACGDPEPAAVPDVASLLTTTTAA
                     PAAPPGPPPECGDPTASSRPDPRLPTSGVMPAGTYMREIQDRGRLVVGVSIDTLLFGS
                     VNPFTGDIEGFDIDILHEISKALFNGDPDRLEYKAINNAERIPKIQDGSVDIVARTMT
                     INCARRELVDFSSVYYDAGQKVLVRADSDAAGIEDLAGQKVCTSASSTSVENLRALAD
                     PPEITEVVDQTDCLRLFQAGDVAAISTDDVILAGFAAQDPNAKIVGARFSDEPYGLAI
                     SLDHPEFTQFVNAVLERMRTDGTWKAIYEKWLGEVYATSQGEGAPIPEPPAATYR"
     gene            4602..>5293
                     /locus_tag="VFX21_10625"
     CDS             4602..>5293
                     /locus_tag="VFX21_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096462.1"
                     /translation="MTLTTAQIDERIKRYRSALEGASANLMAFEGDDAREVVHAAPLQ
                     GTTAKRIADAERVLGELWEDFGLFRAHVDRLTELRGTGRLPGNRVEELDRLLTTAAIA
                     LPPDDVPLATRGLLDPATTANSISADDLLANMVKAFDTAKRDILSIDETWKTMLPALD
                     NVTEQMTRLEHLADDVGGAPRNALADLQRATSALRAKVIEDPLGVSPTALTDLQTQVR
                     TLVDDLDAQRSQR"
BASE COUNT          846 a         1852 c         1801 g          794 t
ORIGIN      
        1 cgcgctgttc cggcacccgc tcggtgtgcc catcccgcac accgcgatca tcacggagcg
       61 caaggaccag ttcggggaga cgctgggcga gttcgtccag gagaacttcc tgtcggcgga
      121 cgcgatcagc gagcgcgtcc gcgcatcgaa cgccgtcgac cgcgcggcga cgtggctcgc
      181 ggatccggtg aacgcgcgca gcgtgtccgc gcacgccgcc gacctcgcgg tcggcctcgc
      241 cgatctcgtg cgcgacgaag acgtccacac cgtgctcgag acggagatcc gcaacgccgt
      301 ggaccgcatc gagctggcgc cgctcgccgg ccgggcgctg aagttcgcga ccgtgcaggg
      361 ccgtcaccag caactgctcg acgcagtgtt gatcggcggc cagctatacc tcgacgagaa
      421 ccgtcagatg ctgcacgacc ggttcgcgca gcaagcaccg tggtggttgc ccggcgcagt
      481 ggaggaccgc atcttcgatc gcatcctcga cggcttccgc gatctcctgc ggtcgatcaa
      541 cgacgacccc aaccacgagt tccgcgccca gttcgacgcg cgcgttgcgg acctcgcgga
      601 gcgcttggag cgggatcccg agatgcgggc tcggggcgaa cagatcaagc aggagctgct
      661 gtcgcacgag cagctacgcg agtggaccgc gtcgttgtgg agcgacgcca agcgcacgct
      721 gcgcgagcag gccggcgacc ccgactccgc ggtgcgcgtg cggctggcgg agggcatcgc
      781 cgcggccggc gcccgcctgc gtgacgatcc caacctcgcg cacaaggccg acgagctgat
      841 cgacgccggc attcgctacg tcgtcgacca cttccagacc gagctcgcgc agctggtgag
      901 cggcacgatc gcccgctggg acggcgagga gaccagtcac cgcctggagc tgctgctcgg
      961 gccggacctc cagttcatcc ggatcaacgg caccgtggtg ggcgcgttgg tgggcttggc
     1021 aatccacacg ttcgcgcaga tgctgtaggt gtgcgcccga ggggacctcg caccgaagac
     1081 gtgctggagc cgctgcccgt catcgaggac ggcgtcgaga tcgcgcccgc gcacgcggcg
     1141 cgggcaacga acgtcagcag cgcagtccgc gagtgtgtcg acgccgtcaa ggcggtgctc
     1201 gacgagagcg gacaggtacg gcgccagatg ccgttcaccc cgaccgaacc accgcggccg
     1261 ccgcgcgttg cgcgcttcgt gtcgacgttc agccgcatga tcgcgctcga gccccgacga
     1321 ctcgaggcac tccccgaatg gtggatgcgc caccagcacg aaggccgcgt gcagatcacc
     1381 cgccggttgt ggttcgaagc accgcgttcg gtcgcgaggg gcgagtggcg cgcgcgaggg
     1441 cggctgcgta gcccgtggct catgcgctcc atcccggtcg agctcgtcat gtggaagcac
     1501 ctcgacgcgt ggacgcgaat gatgctcgaa ccgcagcggt ccgtccacgt gggtcgccgc
     1561 tacttccgcc gcggccaccg cgtgctcgac gaactgacgc ggcagctgct cagtacgtga
     1621 ggaacgcgag ccgacgggtg ccgccgcgcg ctcaaacgtt gcgaggtcgc gagaaatccc
     1681 ggttcggatg gcggggcggc atggcgacgt cgggcaccgg gcacttgctc gccttcatgt
     1741 cctcgagcac gtgcaccatg ttgaggaggt cttcctcgta gctgaacttg ccgttgccgg
     1801 cgtacacgat ggtcgacacg cccgactgct cgaacggccg tccgtcgtcc ttccggcccg
     1861 ggagcacctg cttccacttc acgacgatgg tgtcgccgtc gatcgcgtag aactcgatcg
     1921 ggaacttcca ctcctcgaaa ccgaccatcg cgtcgccgaa gacggtgcgc ttcatctcct
     1981 cgatgccctc cacccggccc catgcgggat cgatgaacac cgcgtcgtcg acgaagaacg
     2041 gcgcgaggtc ggcccacgtg ccctgccccg cgtcgatggc ctcgcgcacg gcgacgtaac
     2101 gctgcatcgt ggcgtcgatc tcgtcacggg gaaacggcat cctggagctc ctcggtcggc
     2161 gggtcactaa ccttgccacg tgcggggcgg cgaccttggg ggtggcgttc ggtgagcgcg
     2221 ggttcggccc cggctgtaca gcagccggcc cctcctccgg cgcgcgcttc gtggggaacg
     2281 cctcgcggcg ggcgcacccg gccgccgacg cgccgggccg gcctcgaccc gcgcccgacc
     2341 ggcctggcca cgccgcgcct gctggggttc gtgaccatcg ggctgctggt cgcgatcgtc
     2401 gcggtgtggg cggccgcgtt cacggccgtg aaccgccggg cggacgcggt gcgcgacgcg
     2461 atgcagcgcg cggaaccgct catcgtctcc gcccagggca tcaacaccga gttggcccgc
     2521 gccgacgcga gcgccgcggc ttcgttcctg gtcggtggcg tcgaagcggc cggcccccgg
     2581 cagcagtact tggacagcat cgatgccgcg atgcgcgagc tctccgtcgc ggcccagaac
     2641 ggcggatcgg tcgaaggtgc gtccgacgcg atcgcgacga tcagcgagaa ggtgcccgaa
     2701 tacgcgggtc ttatcgagag cgcgcgtgcg aacaaccggc agggcttccc catcggcgct
     2761 gcgtacttgc gagccgcgtc cgccttgttg caggacgaga tcttcccggc cgcggagagg
     2821 ctctacgacg cgaccgtcga cgcgtatgcg gacgcgtatg ccgacgcggg tgcggcacgc
     2881 gacgcgccgc tgctcgcctt cgccgggatc gtgctgctcg ccgcgctcgt cgcgacgcag
     2941 attctgttgt tccggcgtac gaatcgcgtg ttcaacgtgc cgctcgtgct cgcgacggtc
     3001 gttgcactcg ttctcgtgtt catgccgctg acggcaatgt cgcacgagca ccgagagctc
     3061 gcgcgcgcga ccgacgaggg gtacttcggc gtcaacacga tcgcacgggt ccgcatcctc
     3121 gccttccgag cgagaggcga cgagagcttc gcgttgattg cacgcggcaa cggggagcgt
     3181 tggtacaccg acttcgacac ggtgatgtcc gcgatcggta gtccggatga cgatggtttg
     3241 ctgggcgccg cgttcgaaga ggaaccgatc gacgcgttct catccgatct cgcggacgcg
     3301 ctcgaccact acaagagcgc acaccaatcc gtccaggaga ccgaccgcgc cggcgacttc
     3361 gacatggcgg tcaccttggc gaccggcgat gccgcgacct cgtcggcgac agcctttgcc
     3421 gcactcgacg aagagctgaa ggcgacgttg gacgaatacg cggcgcggtt cgagcgcggg
     3481 gccgggcggg ccgagaaaca gctcgacaac caggaggtgt gggtctcggt gctcgcgatc
     3541 ctggttgcgt tgggcgtggt cgtcggcctg caacaacgga tccgtgagta ccgatgaggc
     3601 tccgtacgtt catgcgtacc ggtggcgccg cggtcgcggc ggtcatgttg ctcgccgcgt
     3661 gcggcgatcc ggagccggcc gccgtgcccg acgtcgcatc gttgctcacg acgacgaccg
     3721 cggccccagc cgcgccgccc ggtcctccgc ccgagtgcgg cgacccgacc gccagctcgc
     3781 gacccgaccc gcgccttccg acgtcgggcg tgatgccggc gggcacctac atgcgcgaga
     3841 tccaggaccg cggccggctg gtcgtcggtg tgtcgatcga cacgctgctg ttcggttcgg
     3901 tcaacccgtt caccggcgac atcgagggct tcgacatcga catcctccac gagatctcga
     3961 aggcgctctt caatggtgac cccgaccgcc tcgagtacaa ggcgatcaac aacgcagagc
     4021 gcatcccgaa gatccaggac ggatcggtcg acatcgtcgc ccgcacgatg acgatcaact
     4081 gtgcgcgtcg ggagctcgtc gacttctcgt ccgtgtacta cgacgcgggc cagaaggtgc
     4141 tcgtgcgcgc ggactccgac gcggcgggta tcgaagacct cgccggtcaa aaggtgtgca
     4201 cgtccgcgag ctcgacgtcc gtcgagaacc tccgcgccct cgccgaccca ccggagatca
     4261 ccgaagtcgt cgaccagacc gactgcctcc ggctcttcca ggccggcgac gtggccgcga
     4321 tctccaccga cgacgtaatc ctcgccggct tcgccgccca ggacccgaac gccaagatcg
     4381 tgggtgcccg cttcagcgac gaaccgtacg ggctggcaat cagcctcgac catccggagt
     4441 tcacgcagtt cgtgaacgcg gtgttggaac gcatgcgcac ggacggaacg tggaaggcca
     4501 tctacgagaa gtggctgggc gaggtctacg cgacgtcgca gggcgagggc gcgccgatcc
     4561 cggagcctcc ggccgcgaca taccgctagg ccgcaaccgc gatgacgctc acgactgcgc
     4621 agatcgacga acgcatcaag cggtaccgca gcgcgctcga aggtgcgagc gcgaatctca
     4681 tggcgttcga aggtgatgac gcgcgcgagg tcgtccatgc cgcgccgttg caaggcacga
     4741 ccgcgaagcg catcgccgac gccgaacgtg tgctcggcga gctctgggag gacttcgggc
     4801 tgttccgtgc gcacgtcgac cggctcaccg agctgcgggg caccggccga ttgccgggca
     4861 accgggtgga ggagctcgac cgcctgctga ccacggccgc gatcgcgctg ccgcccgacg
     4921 atgtgccgct cgcgacccgc ggtttgctcg accccgcgac gacggccaac tcgatcagcg
     4981 ccgacgacct cctcgcgaac atggtgaagg cgttcgacac cgccaagcgc gacatcctgt
     5041 cgatcgacga gacgtggaag acgatgttgc ccgcgctcga caacgtcacc gagcagatga
     5101 cgcgcctcga gcatctcgcc gacgatgtcg gtggcgcgcc gcgcaacgca ctcgcggact
     5161 tgcagcgcgc gacatccgcg ctgcgcgcca aggtcatcga ggacccactt ggtgtctcgc
     5221 cgaccgcgct caccgatctg cagacgcagg tacgcacgct ggtcgacgac ctcgacgccc
     5281 agcgctccca acg
//
LOCUS       DASUGA010000536         2420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1392924, whole genome shotgun sequence.
ACCESSION   DASUGA010000536 DASUGA010000000
VERSION     DASUGA010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392924"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VFX21_10630"
     CDS             complement(<1..504)
                     /locus_tag="VFX21_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX5096463.1"
                     /translation="MSVLVEHEGRVTIVTINRPAVRNAVDGETAAKLADAFRAFDADG
                     DHDVAVLTGADGCFCAGADLKAVSDGRGNPVHEDGDGPMGPTRMLLSKPVIAAVEGFA
                     VAGGLELALWCDLRVAATDAVFGVYCRRWGVPLIDGGTIRLPRLIGQSRALDLILTGR
                     GVAGDEAL"
     gene            complement(501..1112)
                     /locus_tag="VFX21_10635"
     CDS             complement(501..1112)
                     /locus_tag="VFX21_10635"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5096464.1"
                     /translation="MDLLWIRHAEPERIAGGTGVPANPGLTERGQQQAQRLADWLAHE
                     HVDVVLSSPQRRAQETAAPVAAAHGLDVVIHDGLVEYDVQNDSYIPMEELRANNDPQL
                     LAMAEGRWEAFGGETPGEFRDRIAKTIDDIVGSYPGQRVVAVCHGGVVNVAMGIVLGL
                     PAERPFFFDPHYTSLSRMVASRTGVRSIASVNERSHLEARRTP"
     gene            complement(1115..1735)
                     /locus_tag="VFX21_10640"
     CDS             complement(1115..1735)
                     /locus_tag="VFX21_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX5096465.1"
                     /translation="MIFDLGGVVFPSPFEAFDQYDHGNNLAKGTMRALIRRSSEEGAW
                     AALERGELGMDDFFVALEAEATLAGFKLDARRLMGLIGSSLGPRPEMQRAIECIRAKG
                     LLTAALTNNWADEERRSTPHALEGSGLFDVIVESAREGLRKPDPRIYALALARLNVLA
                     SEAVFLDDLGINLKPAREMGMTTIKVVDPKRALAELESVLGFDVGL"
     gene            complement(1747..>2420)
                     /locus_tag="VFX21_10645"
     CDS             complement(1747..>2420)
                     /locus_tag="VFX21_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019868818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5096466.1"
                     /translation="EAMTSAGVDDGSALLVLGFESADHPVDAWIERGVEIARAHGGVA
                     PDGIAISGPRDDGGRAGSDGAVGAWRSAFLRAPYGRDAMVRMSVIVETFETACTWDQF
                     PGLHADVTKSVVQTALAVGADPAVITCRFTHVYPDGPAPYFTVLAPGREPDQLVQWQA
                     IKDAASAALARGNGTITHHHAVGRDHRPWYDEQRPAPFAAALRAAKQTVDPSGILNPG
                     VLIDA"
BASE COUNT          383 a          844 c          811 g          382 t
ORIGIN      
        1 gagcgcttcg tcacccgcga cgcctcggcc ggtgaggatg aggtcgagcg cgcgcgactg
       61 cccgatcagg cgcggcaggc ggatcgtgcc cccgtcgatc aggggcacgc cccagcgccg
      121 gcagtacaca ccgaacaccg cgtcggtcgc cgcaacgcgc aggtcgcacc acagtgcgag
      181 ctcgaggccg ccggcgaccg cgaaaccctc gaccgctgcg atgacgggct tcgagagcag
      241 catgcgcgtc gggcccatcg gaccgtcacc gtcttcgtgc acgggattgc cgcgcccgtc
      301 gctcacggct ttgaggtccg cgccggcgca aaagcatccg tccgcaccgg tgaggaccgc
      361 gacgtcgtga tcgccgtcgg cgtcgaacgc gcgaaaggcg tcggcgagct tcgctgcggt
      421 ttcgccgtcg accgcgttgc gcaccgccgg ccggttgatc gtgacgatcg tgacgcgacc
      481 ttcgtgttcg acgagcacgc tcatggcgtt ctccgagctt cgagatgcga acgctcgttc
      541 acggacgcga tcgatcgaac gccggtgcgc gacgcgacca tgcgcgacag cgacgtgtag
      601 tgcggatcga agaagaacgg ccgctcggcc ggtagaccga gcacgatccc catcgcaacg
      661 ttgaccacgc cgccgtggca cacggccacg acgcgttggc ccggatagga accgacgatg
      721 tcgtcgatcg tcttggcgat ccgatcgcga aattcgcccg gcgtctcgcc gccgaacgcc
      781 tcccagcggc cctcggccat cgccagcaac tgcggatcgt tgttcgcgcg cagctcttcc
      841 atcgggatat agctgtcgtt ctgcacgtcg tactccacga gcccgtcatg gatgacgacg
      901 tccaggccgt gcgcggccgc aaccggcgcc gccgtctcct gcgcgcgccg ctgcgggcta
      961 ctgaggacga cgtcgacgtg ttcgtgcgcg agccagtcgg ccaaccgctg ggcctgctgt
     1021 tgcccgcgct cggtcagtcc cggattcgcg ggcacgccgg ttccgcccgc gatccgctcc
     1081 ggctcggcgt gcctgatcca caacagatcc atggtcagag tcctacatcg aatcccagga
     1141 cggattcgag ttcggcgagg gcacgcttcg ggtcgacgac cttgatggtc gtcatgccca
     1201 tctcgcgggc gggtttgagg ttgatgccga ggtcgtcgag gaacaccgcc tcggacgcga
     1261 gcacgttcag gcgcgccagc gccaacgcgt agatgcgcgg gtcgggcttg cggagtcctt
     1321 cgcgcgccga ctcgacgatg acgtcgaaca agcccgagcc ttccagtgcg tgcggggtcg
     1381 agcgtcgctc ttcgtcagcc cagttgttcg tgagcgcggc ggtcaacaga cccttggcgc
     1441 ggatgcactc gatcgcccgt tgcatttcgg gtcgcggacc gagcgacgaa ccgatgaggc
     1501 ccatcaagcg gcgcgcgtcg agcttgaacc cggccaacgt cgcttcagct tccagcgcaa
     1561 cgaagaaatc gtccatgccg agctcacccc gctcgagcgc cgcccatgca ccttcctcgc
     1621 tgctgcgccg gatgagcgcg cgcatcgtgc ctttggcgag attgttgccg tggtcgtact
     1681 gatcgaaggc ctcaaacggc gagggaaaca cgacgccgcc caggtcgaag atcaccgctt
     1741 tcaggatcat gcgtcgatca acacgcccgg attgaggatg ccgctcggat ccacggtctg
     1801 tttcgcggcg cgcagtgcgg cggcgaatgg cgcgggccgc tgctcgtcgt accacgggcg
     1861 atgatcccgc ccgacggcgt ggtgatgcgt gatcgtcccg ttgccgcgcg cgagggcggc
     1921 actcgctgcg tccttgatcg cttgccactg gacgagttga tcgggttcgc gcccgggggc
     1981 gagcaccgtg aagtacggcg ccgggccgtc tggatagacg tgcgtgaacc ggcacgtgat
     2041 gacggcaggg tcggcgccca cggcgagcgc agtttggacg accgacttgg tgacgtcggc
     2101 gtgcagaccc gggaactggt cccacgtgca cgcggtctcg aacgtctcga cgatgacact
     2161 catgcgcacc atcgcgtcac ggccgtaggg cgcgcgcagg aaggcactgc gccacgcgcc
     2221 cacagcaccg tcgctgccag cccggccacc gtcgtcgcgc gggccgctga tggcgatgcc
     2281 gtccggtgcg acgcccccgt gggcacgagc gatctcgacc ccgcgctcga tccacgcatc
     2341 caccggatga tcggccgatt cgaagcccaa cacgagcaag gcggagccgt cgtccacgcc
     2401 cgcggaggtc atcgcctccg
//
LOCUS       DASUGA010000537         2303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_993384, whole genome shotgun sequence.
ACCESSION   DASUGA010000537 DASUGA010000000
VERSION     DASUGA010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993384"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VFX21_10650"
     CDS             <1..553
                     /locus_tag="VFX21_10650"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="catalyzes the formation of alpha-D-galactose
                     1-phosphate from D-galactose in galactose metabolism;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEX5096467.1"
                     /translation="RAHHALMIDCRTFTIDEVPMPDDVRVLIVHSGVPRTLESSPYAQ
                     RRAEGMAIAAELGLSVLRDATIEQVQERPRGRHVVSEMARVLAFADALRAGDVVRCGE
                     LMCASHASLRDDMEVSLPPLDALVDLLVREGAYGARLTGGGFGGCVVALVPERDADRI
                     AHAVGDRQFWIVGAADGAGPCLS"
     gene            complement(546..1388)
                     /locus_tag="VFX21_10655"
     CDS             complement(546..1388)
                     /locus_tag="VFX21_10655"
                     /inference="COORDINATES: protein motif:HMM:NF039362.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HEX5096468.1"
                     /translation="MFSRSWMLAKASWGVLRQDKELALLPLISFAAWIAVAATFLLPI
                     ALVANDSAEQDSWTQNPVTWVLAFAFYVTASYVMIFFNAAIVSGADERMRGGDPTLGS
                     AIKGAADRAMVLLPWAVVSASVSLVLRAIEQRAGIVGRIVAGLIGLAWSLVTFLVIPI
                     LVVEGISVGQAIKRSTALFKGVWGEQVVTNGGISLVSFAAVIVGVLVALPFFALGGIF
                     AVVGVAALVAWVGFVVCFSAALSGVFQLALYRYAVDGRVPGFDEDLMRNAFRPRGDRG
                     GWLN"
     gene            1466..>2303
                     /locus_tag="VFX21_10660"
     CDS             1466..>2303
                     /locus_tag="VFX21_10660"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HEX5096469.1"
                     /translation="MRVRAPGRVNLIGDHTDYQDGLCLPMAIDREVVVDWAPSTASVS
                     SFRSAEPESGLVAAAPAVLAERGIVPRIVDAAVTSTIPVGGGLSSSAAFTVACLLALT
                     DELSGRELALAAQAVEQRATGVPCGVMDQMASVCGRPDHALLLDCRTLAVTPVPLPPG
                     CAVIVVHSGLPRRLATSAYAKRRAACERAAARLGVAALRDASLSDVADDPMPRHVVSE
                     NARVLAAADALRAGDVAMFGRLMDESHASLRDDFAVSTPELDEVVEHCRAEGAYGARL
                     TGA"
BASE COUNT          336 a          815 c          835 g          317 t
ORIGIN      
        1 gcgcgcgcac catgcgctga tgatcgactg ccggacgttc acgatcgacg aagtgccgat
       61 gcccgacgac gtccgggtgc tgatcgtgca ctcgggcgtg ccgcgcacgc tcgagtcgtc
      121 gccgtacgcg caacggcggg ccgagggtat ggcgatcgcc gccgagctcg ggctctccgt
      181 attgcgcgac gccaccatcg aacaagtgca agagcggccg cgcggccgtc acgtcgtgtc
      241 ggagatggcg cgcgtcctgg cgttcgcgga tgcgctgcgc gccggcgacg tcgtccgctg
      301 cggcgagctg atgtgcgcga gccacgcgtc actgcgcgac gacatggaag tctcgctccc
      361 cccgctcgac gcgctggtcg acctcctcgt gcgcgagggc gcgtacggcg cgcggctcac
      421 gggcggcggt ttcggcggct gcgtggtcgc gctcgtgccc gaacgcgacg ccgaccgcat
      481 tgcgcatgcg gtcggcgatc gtcagttctg gatcgtcggc gccgcggacg gcgccggacc
      541 ttgtctcagt tgagccagcc gccccggtcg ccgcgggggc ggaacgcatt gcgcatcagg
      601 tcttcgtcga agcccgggac gcgcccgtcg accgcgtagc ggtacagcgc gagctggaac
      661 acgccgctga gcgcggccga gaagcacacg acgaagccga cccacgcgac gagcgccgcg
      721 acgccgacga cggcgaagat gccgccgagc gcgaagaacg gcagcgccac gaggacgccg
      781 acgataacgg ctgcgaagct gacgaggctg atgccgccgt tggtgacgac ctgctcgccc
      841 cacacgccct tgaacaacgc cgtcgagcgc ttgatggcct gcccgaccga gatgccttcg
      901 accacgagga tcgggatcac gaggaacgtg accagcgacc acgcgagtcc gatgaggccg
      961 gcgacgatgc gaccgacgat gccggcgcgc tgttcgatgg cccgcaggac gagcgagacg
     1021 gacgcgctca cgacggccca cggcagcagc accatggcgc ggtcggcggc acccttgatc
     1081 gcggaaccga gcgtggggtc gccgccgcgc atccgctcgt cggcgccgga cacgatcgcg
     1141 gcgttgaaga agatcatcac gtacgacgcg gtgacgtaga aggcgaacgc gagcacccac
     1201 gtgacggggt tctgcgtcca gctgtcttgt tccgccgagt cgttggcgac gagcgcgatc
     1261 ggcaagagga aggttgccgc gaccgcgatc cacgcggcga acgagatcag cgggaggagc
     1321 gcgagttcct tgtcctggcg caggactccc cacgatgcct tggcgagcat ccacgaacga
     1381 ctgaacattc ccacccgacg gacctcctgg tcggtgttcg ccaccagcgt accggcctcc
     1441 cggccgcctc ctaacctgcc cgcccgtgcg agtgcgtgcg cccggccggg tgaacctgat
     1501 cggtgaccac accgactacc aggacggcct gtgcctcccg atggcgatcg accgcgaggt
     1561 ggtcgtcgac tgggcgccga gcactgcgtc cgtgtcgtcg ttccggtcgg ccgaacccga
     1621 gagcgggctc gtggccgccg cgccggccgt gctcgccgag cgcgggatcg tgccccggat
     1681 cgtcgacgcg gccgtgacgt cgacgatccc cgtcggcggc ggcctgtcgt cgagcgcggc
     1741 gttcaccgtc gcctgcctgc tcgcgttgac cgacgagctg tcgggtcgcg agctcgcgct
     1801 cgccgcgcag gccgtcgaac agcgggccac cggtgtgccg tgcggcgtga tggaccagat
     1861 ggcgtcggtg tgcggccgac cggatcacgc gctgctcctc gactgccgga cactcgccgt
     1921 cacgccggtg ccattgccac ccggctgcgc ggtcatcgtc gtgcacagcg gtttgccgcg
     1981 ccgcctcgcg acgagtgcgt acgcgaaacg acgggccgcg tgcgagcgcg ccgcggcccg
     2041 gctcggcgtg gccgcgttgc gcgacgcgtc gctgtccgac gtcgccgacg acccaatgcc
     2101 acgccatgtc gtgtcggaga acgcgcgggt gctcgccgcg gccgacgcgc tgcgcgccgg
     2161 cgacgtcgcc atgttcggac ggttgatgga cgagagccac gcgtcgttgc gcgacgactt
     2221 cgcggtgtcg acgccggagc tcgacgaggt ggtcgagcac tgccgcgccg agggcgcgta
     2281 cggcgcgcgg ctcacgggcg cgg
//
LOCUS       DASUGA010000538         3426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1933220, whole genome shotgun sequence.
ACCESSION   DASUGA010000538 DASUGA010000000
VERSION     DASUGA010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1933220"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VFX21_10665"
     CDS             <1..669
                     /locus_tag="VFX21_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096470.1"
                     /translation="SRLVHAELTRLGVRVQLVPTLVPRSAFDDWRTTRIRRATVDMIG
                     WHVGDGGDRDRSELEPAAQALTTALRNHLELRIDLTGDPNRMPELLLHHRRVRVIVGP
                     PSRSDVARWDLQLWTPDHVRCEIAGYLEPVVVAGAAGVPTAAGAASGAARDALLSAAT
                     TVPNTASANSWSRVIEHFLGDARQLRRESATVSRRVESLFGAAAASTVLNRFFGWATN
                     GAEA"
     gene            666..2732
                     /locus_tag="VFX21_10670"
     CDS             666..2732
                     /locus_tag="VFX21_10670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5096471.1"
                     /translation="MSDFDVTIIVPIYRGVEEVQRCLASVVRHAKRTQAAAELLLLND
                     GSPVEEMHAMLQRFVDETTALPVRLLRNEQNLGFVKTVNRGMRAATGDVVVLNSDTVV
                     TAGWLDSLVRTASADDVATVTPLTNFGSLATLPPSIIRAFGLDSDAPRIDEAAAFVSR
                     HSARLCPEVIAGVGFCMYITRRALDICGELDEATFGRGYGEEVDFCLRASRVGFRHLI
                     DDATFIYHEGGVSFGAEREEGMMRGSELLHARYPFFRPANTKERRENPLRTSFAALEL
                     GMTERDTRRPHVLHLLHPPSVIGGTEKHLDSLLRALFDEFDAAVLYPNRESNFVLRTM
                     WNVSGESIEHEFLIPGAPRTVTKAYDEVSAAALETALTMFDFDAVHVQNLIGHSLSSF
                     DVLASFDGPVVCSVRDLYLACPNHSLLYMNQEPCGIPDDLSFCRRCLPATREEPLEYL
                     LAFREIVESRLDAVDHWVFASQSAADYFFRAYEVDAERVELIPHGAIIDAGFRVQDVD
                     EGLIFDEPLRFAFVGLGWTKKGLAVVNELAERFADTAIEFHHFGELKERASPYLRCHG
                     PYDNEVLPFLLQRAGIQVVLLPGPYAETFGHVMTEAIVGGIPVIGATYSALGERLRAS
                     RAGWTVDPANIDEIEDLVRNLDRSRIEILRATRAAIAAPLRSVAQTADRYAALYRSAE
                     RTPATT"
     gene            2784..>3426
                     /locus_tag="VFX21_10675"
     CDS             2784..>3426
                     /locus_tag="VFX21_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096472.1"
                     /translation="MSAVNRQLQAQLEGADIRAVALPANGRGTDDMLAVTQEATIVAP
                     SRRQNLGESYIEQLEMHGESGSATLRRTPKGTYVVVEGGRRREVKSGILGAAIERALG
                     RAAQITKDELDTLPAGVPVEVLEGPTGAPFVVIAGRRWPIRGLPLPHPVRAEHVQVFP
                     EGDELNVAFASVPRTRLDDAMYGRYQIARFKAAARRSGAVGLARKTASKAKRKL"
BASE COUNT          576 a         1168 c         1114 g          568 t
ORIGIN      
        1 agccgcctcg tccacgcgga gttgacgagg ctcggcgttc gggtacagct cgttcccaca
       61 ctcgtcccgc gctcggcgtt cgacgactgg cgaacgactc gcatccggcg cgccaccgtc
      121 gacatgatcg gctggcacgt cggcgacggc ggtgaccggg atcggagcga gctggaaccg
      181 gccgcgcagg cactcacgac ggcattgcgc aaccacctcg agctcaggat cgatctgacc
      241 ggcgacccga accgcatgcc cgaactgctg ctccatcacc ggcgggtccg ggtcatcgtc
      301 ggtcccccgt cacgatccga cgtcgcacgc tgggatctgc agctctggac tcccgaccac
      361 gttcgctgcg agatcgcggg gtacctcgaa cctgtcgtcg tcgccggagc tgccggcgta
      421 ccgaccgccg cgggggcggc cagcggtgcg gcgcgtgacg cgctgctcag cgccgcgacc
      481 acagtcccga acactgccag cgcgaactcg tggtctcgcg tcatcgagca cttcctcgga
      541 gacgcacgcc aacttcgtcg cgagagcgca acggtgtcac ggcgcgtcga gtcgttgttc
      601 ggcgcggcgg cggcgtccac tgttctcaac cgcttcttcg gttgggcgac gaatggagcc
      661 gaagcatgag cgacttcgac gtcacgatca tcgtcccgat ctatcgcggt gtcgaggagg
      721 tgcagcgctg cctcgcgagc gtcgttcgcc acgccaagcg cacgcaggcc gccgcggagc
      781 tcctgttgct caacgacggc tccccggtcg aagagatgca cgcgatgctg cagcgcttcg
      841 tcgacgagac gacggccctg ccggttcgcc tcctgcgcaa cgaacagaac ctcggattcg
      901 tgaagaccgt caaccgcggc atgcgggccg cgaccggcga cgtcgtcgtt ctcaactccg
      961 acaccgttgt cactgccgga tggctcgatt cgctcgtacg caccgcgagc gccgacgacg
     1021 tcgccaccgt gacgccgctg acgaacttcg gttcgttggc aacactgcct ccgtcgatca
     1081 tccgggcgtt cgggctcgac tccgacgcac cacgaatcga cgaggccgcg gcgttcgtct
     1141 cacgccattc ggctcgtctg tgccccgagg tcatcgcggg cgtcgggttc tgcatgtaca
     1201 tcactcgccg ggcattggac atctgcggcg agctggacga agcgacgttc ggtcgcggct
     1261 acggcgaaga ggtcgacttc tgcctgcgcg cgagtcgcgt cggatttcgg catctcatcg
     1321 acgacgccac attcatctac cacgagggtg gcgtgtcgtt cggagccgag cgcgaagaag
     1381 gcatgatgcg cgggtcggag ctgctgcacg cgcgctaccc gttcttcaga ccggcgaaca
     1441 ccaaggaacg gcgagagaac ccgctgcgca cgagcttcgc cgcgctcgag ctcggcatga
     1501 ccgaacgcga cacgcggcgt ccgcacgtgc tgcacctcct gcatccccct tcggtgatcg
     1561 gaggcaccga gaagcacctc gacagcctgc tccgggcgct gttcgacgag ttcgacgcgg
     1621 ccgtcctgta cccgaatcgc gagtcgaact tcgtcttgcg cacgatgtgg aacgtcagcg
     1681 gcgagtcgat cgagcacgag ttcctgatcc cgggtgcgcc acggacggtc acgaaggcat
     1741 atgacgaagt cagcgcggcg gcgctcgaga ccgcgctgac gatgttcgac ttcgacgcgg
     1801 tgcacgttca aaacctcatc ggacattcgc tttcgtcttt cgacgtcctc gcgtcgttcg
     1861 acgggccggt ggtgtgctcc gtgcgagacc tatacctcgc gtgcccgaac cattcgttgc
     1921 tctacatgaa ccaggagccg tgcggtatcc ccgacgacct ctccttctgc cgccgctgcc
     1981 tgccggcgac tcgcgaggaa ccgctcgagt acctcctggc gttccgtgag atcgtcgagt
     2041 cccggctcga cgcagtggac cattgggtct tcgcgagcca gagcgcggcg gactacttct
     2101 tccgcgcgta cgaggtcgac gccgagcgcg tcgagttgat tccacacggc gccatcatcg
     2161 acgccggctt ccgcgtgcaa gacgtggacg aaggtctcat cttcgacgag cccttgcgct
     2221 tcgcgttcgt cggcctgggc tggacgaaga aggggctcgc ggtcgtcaac gaattggccg
     2281 aacggttcgc cgacacggcc atcgagttcc atcacttcgg ggagctcaag gaacgcgcat
     2341 ccccgtacct ccgctgccat ggcccctacg acaacgaagt gctgccgttt ctcctgcaac
     2401 gcgccggcat ccaggtcgtt ctgcttccgg gcccgtatgc cgagacgttc ggccatgtca
     2461 tgacggaagc gatcgtgggt ggcattcccg tgatcggcgc gacgtacagc gcgctcggcg
     2521 aacggctccg tgccagtcgc gccggctgga ccgtcgatcc cgcgaacatc gacgagatcg
     2581 aggatctcgt acgcaatctg gatcgatcga ggatcgagat cctgcgcgcg actcgggcag
     2641 caatcgcggc accgctgcgt tcagtcgcgc agacagcaga tcgctacgcg gccttgtacc
     2701 ggtcggccga acgcacgccc gcgacgacgt agaggagcat ggtgtccgac gaaatcactc
     2761 tcttgaaacg acagctgcga gcaatgagcg ccgtcaaccg tcagctgcaa gcgcagctcg
     2821 agggcgccga catccgggcc gtggcgctcc cggcgaacgg gcgtggtacc gacgacatgt
     2881 tggcggtgac ccaagaggcg acgatcgtcg caccttcccg ccgccaaaat ctcggcgagt
     2941 cgtacatcga acaactcgag atgcacggtg aatcgggctc ggcgacgttg cggcggacgc
     3001 cgaagggaac gtatgtcgtc gtcgagggtg gccgccgccg tgaggtcaag tccgggatcc
     3061 tcggggccgc gatcgagcgc gcactcggac gcgcggccca gatcacgaag gacgagctcg
     3121 acacgttgcc ggccggtgtg cccgtcgaag tcctcgaggg gccgacgggg gcgccgttcg
     3181 tcgtgatcgc gggccgaagg tggccgatcc ggggcctgcc gctgccgcac ccggtacgcg
     3241 cggaacacgt acaggtcttc ccggagggcg acgagctcaa cgtcgcgttc gccagcgtcc
     3301 cccgcacgcg gctcgatgac gcgatgtacg gccgctacca aattgcccgg ttcaaggcgg
     3361 ccgcccggcg gagtggtgcc gtcggtctcg cccggaaaac cgcgagcaag gcaaaacgga
     3421 aactcg
//
LOCUS       DASUGA010000539         9688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_268619, whole genome shotgun sequence.
ACCESSION   DASUGA010000539 DASUGA010000000
VERSION     DASUGA010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 9688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9688
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268619"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="VFX21_10680"
     CDS             <1..1344
                     /locus_tag="VFX21_10680"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5096473.1"
                     /translation="TTAPALVVDPASFGQPWGSTVTGLLTFRGNPTRSYHGQGPVPAQ
                     PKVRWKFPDAAMCGQSAEGGETRTWCGTGWTGQPAVFERDGRTWVVFGAYDYKVHFVD
                     AETGERILPDFPTGDIIKGSPTIDPDDYPLVYIGSRDNNLRVLAIDRPEATELWRINA
                     NDVSPTLWNNDWDGSPLVVNDHLLEGGENSQVHIAKLNRAYGADGLVTAAPSLVFHAP
                     GWDDELLRDLGDRTVSIEESVTVAGDTAWFANSGGLLQGWDVSFLRTGTGTPTRTFRY
                     WLGDDVDASIVADADGYLYVGVEHERGNARSNEVGQLLKIDPRVPDAPIVWSVKSKGS
                     DTSGTWSTAAVLDDTVIWPTRPGTIYGIDRASGAVRWTLDLPPPLMGSPVVGDGVWIQ
                     GDCNGVLHGFDVHEPHVQPRELWSVELGGCIESTPAVWKGRIFVGTRAGLEYALD"
     gene            1354..1776
                     /locus_tag="VFX21_10685"
     CDS             1354..1776
                     /locus_tag="VFX21_10685"
                     /inference="COORDINATES: protein motif:HMM:NF016102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HEX5096474.1"
                     /translation="MLIAAALIVMGILEIYVIVQVGQAIGALYTIALLILMGILGVWL
                     AKREGFAVFRRFRRTTSEGRVPTSEVIDGFLIFTGAVLMIIPGFISDVIGILLLIPPT
                     RAIPRNFIKRRFRITIIDQYGPQGRGPRPVPPDDIIDI"
     gene            complement(1833..2687)
                     /locus_tag="VFX21_10690"
     CDS             complement(1833..2687)
                     /locus_tag="VFX21_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010540131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX5096475.1"
                     /translation="MALSRLAASAPPEQIVGALERDGAVIVDDLLDADVLARFNAEID
                     PFLEAAKPAKERPFINPAIQYFFGAQTRDVTGVAGRSRVFSTEILTHPVYKAVCDAIL
                     LPHCATYQLNIAHVLDRGPGSDDQFMHRDELVWVHLPKPHPEIQLASVIALVDFTADN
                     GATRVVPGSHKWTDDRSPTPDEIEAAEMRAGSAIVYLGSTLHGGGANVTTGTFRRGMH
                     VSYCVGWLRTEENQYLATPLDFVRTLPPESQALLGYAAHDAVHMGGGYLGTVAVRDPV
                     ELLAAGEL"
     gene            complement(2706..3521)
                     /locus_tag="VFX21_10695"
     CDS             complement(2706..3521)
                     /locus_tag="VFX21_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096476.1"
                     /translation="MTWDVTPAERLRSVTRRGVADHVLAAEAAGALAGFASEPASLVV
                     ACRRVLAHHRAHGALWWVCARILAAPEPNVAAREAMASLDADRTANRLAATLPLLDDG
                     DLVATIGWPHAVDEALAERQDIAVLSVRVEGVDAFGDLRYRQTDRNVPVVDVWDIEAL
                     ALLLVPAFAIGPDRTLVPAGTDALLRDLGGRARDVWLVGGVGRVLPARLFDAARTAAE
                     HVDEMLDPEEAAPDVVSLERIDRIAGPRGVELPTDAAARIDCPIVPELLRPLD"
     gene            complement(3531..3929)
                     /locus_tag="VFX21_10700"
     CDS             complement(3531..3929)
                     /locus_tag="VFX21_10700"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX5096477.1"
                     /translation="MSSFLGEDWLAEMAAAAAQAPGAAGVDRLVIENVVTGVPGRGEV
                     RYCLVVTNEALRVEVGAAMPPGDVTFVTGYETAVMLARGETNAQQALADGCLTVRGSI
                     GALGAGASALAALDDVFAAVRAGTTYAPAR"
     gene            3965..5044
                     /locus_tag="VFX21_10705"
     CDS             3965..5044
                     /locus_tag="VFX21_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013735118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX5096478.1"
                     /translation="MSRREATFPPALVTVADRAEARILDLLDREVDRWRGVDAALVAP
                     LDALREFVVAGGKRLRPAFCHCAFVGAGGAPDDPAVIDAAAALELIHTFALVHDDVMD
                     GSDTRRGQDAVHRSFARRHRDGSWRGEARRFGDGMAILVGDFAHVYADMLMRSALMRG
                     TPPAAIEVYDTLRVELCVGQSLDLITTARSGTDGEAARRIATYKSAKYTVERPMHLGA
                     ALAGRLDELSDPLSAIGLPLGAAFQLRDDVLGAFGDSDVTGKPVGDDLREGKATPLVA
                     LAAERAEPADAPLFARLGAVDLQPGEVAALQDLFVRTGALKDIEREIEQLVDVARTAI
                     DAAPLSPEARERLSELADYVAWRDR"
     gene            5045..5989
                     /locus_tag="VFX21_10710"
     CDS             5045..5989
                     /locus_tag="VFX21_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5096479.1"
                     /translation="MPGEMHDDEPAIDVVDLEKRYGTVQAVAGLSFSVSRGEVFGLLG
                     PNGAGKTTTVEILEGYRRPDGGHARVLGLDPVTDGAQLRPRIGVMLQDSGLYPGLRPL
                     ELLRLFAAYYDNPQSPEALLELVGLAESTGTTVRRLSGGQAQRLSLACALIGRPDVVF
                     LDEPTAGMDPHARATTWELVRSLAANGTTVMLTTHGMDEAEQLCDRVAIITGGRLAAL
                     GTPAELTRHAAADELWFAAVAALDVRLLEKALGVAPDTVVEERAGEYLVRAAATPELI
                     ADLACFLRDQGVVLGALQAGRRSLEEVFLQITAEREPR"
     gene            5986..6714
                     /locus_tag="VFX21_10715"
     CDS             5986..6714
                     /locus_tag="VFX21_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013425967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5096480.1"
                     /translation="MSTRAISAQARAEFVLQLRRGENAIVTLAIPLGVLIFFSKVDAV
                     STNLDDPVDFLVPGVLSLGVMAAAMVSLGIATGFERRYGVLKRLGSTPLSRFGLLTAK
                     TATVLALEVIQLVLVVVTGIALGWDVNGGVVPALGLLLLGTVAFAGIGLLMAGTLRAE
                     ANLALSNGLFLVLLFLGGMAYPLDKLPGALEAFAKLLPAAALSETVRSVLSSEPFPGG
                     ELVVLVVWAIAAPLAAARWFRWEE"
     gene            complement(6751..7332)
                     /locus_tag="VFX21_10720"
     CDS             complement(6751..7332)
                     /locus_tag="VFX21_10720"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX5096481.1"
                     /translation="MGRGRVAAVVAVAVLVPLALLSVILVANRDDDNDAAATTSMTMP
                     AGAMVAGKADVGSMAPDFTLPSLGGEVVSLAQLRGTPVVLTFYASWCYDCSQELPRLQ
                     HVRDERGDDVAIVGVSYRDLIGDSRDFVDELGLTFPMLHDEDDDVASMYGVRAIPQTF
                     FIDAGGTIRARRFGAPSQDELDQLVDHAAPAPP"
     gene            complement(7339..8247)
                     /locus_tag="VFX21_10725"
     CDS             complement(7339..8247)
                     /locus_tag="VFX21_10725"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX5096482.1"
                     /translation="MVQPALRLDVESRRPLAATIGAYFRLTKPRVIELLLVTAVPPMI
                     LAEGGLPEWSLILAVVVGGALAAGGANTINCWIERDRDQLMRRTHGRPLPMGELNPTH
                     ALVFGIVLNVIAFALLATVANLLAACLTLSATLFYVFVYTIWLKPRTVQNIVIGGAAG
                     AVPALVGWAAVRNDLAAPAWVLFLVVFLWTPAHFWALALKYRDDYAAAGIPMLPVVAG
                     VDMTAKQICGYAMVTVAASLALVLTGWVGLVYALTAAAIGAAFVLQALALRRDPTPKR
                     AIRFFAFSNIYLMVVFVAVAVDALVG"
     gene            complement(8273..8656)
                     /locus_tag="VFX21_10730"
     CDS             complement(8273..8656)
                     /locus_tag="VFX21_10730"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX5096483.1"
                     /translation="MRRFSRPLLIVATLAAASVPAGCGGGGGGGGGKTVDATGGKVTV
                     EAHDIKYDIGKINADAGTLEVTLLEEGKQQHTFVIEGVDGKAAVSPSEKSDSASFELQ
                     PGEYEYYCDIPGHKGQGMKGTLVVK"
     gene            8802..9095
                     /locus_tag="VFX21_10735"
     CDS             8802..9095
                     /locus_tag="VFX21_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX5096484.1"
                     /translation="MTADRLLVTPSTEAGERKSRAGILIPATADQSRRLFWGEVVAAG
                     PTVRSIEQGDRVLFSPDSGFEVEIRGDEYLILRERDVHAVASKRDEGQAGLYL"
     gene            9092..>9688
                     /locus_tag="VFX21_10740"
     CDS             9092..>9688
                     /locus_tag="VFX21_10740"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5096485.1"
                     /translation="MIDPESALRIDGQAVLVTGGTRGIGRAIAEAAGAAGASVAVLAR
                     KPDELEETAKAIAAIGAPVTTVQGSVGDPEMPERAVAAMLDAFGRCDAVVNNAAVNPA
                     FGPLMDADMGAVAKVWDANIAGPLRFARAAWHGWMKDNGGNIVNVVSVGGIRPGPFIG
                     AYNVSKAALIHLTLQLAQELAPTVRVNAVAPALVKTDMA"
BASE COUNT         1474 a         3336 c         3458 g         1420 t
ORIGIN      
        1 acgaccgcac ccgctctcgt cgtcgatccg gcttccttcg ggcagccgtg ggggagcacc
       61 gtcaccggct tgttgacgtt ccgcggtaat ccgacgcgct cgtatcacgg gcaaggcccg
      121 gtccccgcgc aaccgaaggt gcgctggaag ttccccgacg cggcaatgtg cggtcagtcg
      181 gccgaaggcg gcgagacgcg tacatggtgc ggcacgggct ggacgggaca acccgcggtc
      241 ttcgaacgtg acgggcgcac gtgggtcgtg ttcggcgcgt acgactacaa ggtgcacttc
      301 gtcgacgcag agacgggcga gcgcatcctc cccgactttc ccactggcga catcatcaag
      361 ggctcgccga cgatcgatcc cgacgactac ccgctcgtgt acatcgggtc gcgcgacaac
      421 aacctgcgcg tgctcgcgat cgaccggccc gaggcgacgg agctgtggcg gatcaacgcc
      481 aacgacgtca gcccgacgtt gtggaacaac gactgggacg ggtcgccgct cgtcgtcaac
      541 gatcatctgc tcgaaggcgg tgagaacagc caggtccaca tcgcgaagct caaccgcgcg
      601 tacggcgccg acggattggt gacggccgcg ccgtcgctcg tgttccacgc gccgggatgg
      661 gacgacgaac tgctacgtga cctcggcgac cgcacggtct cgatcgagga gtcggtcaca
      721 gtcgcgggcg acaccgcgtg gttcgcgaac tcgggcggcc tgctgcaggg ctgggacgtc
      781 tcgttcctgc gcaccggcac cggcacgccg acccgcacgt tccgctactg gctcggcgac
      841 gacgtcgacg cgtcgatcgt cgccgacgcg gacggctacc tgtacgtggg ggtcgagcac
      901 gagcggggca acgcccgttc gaacgaagtc gggcagttgt tgaagatcga tccgcgtgtt
      961 cccgatgcgc cgatcgtgtg gtcggtgaag agcaaaggga gcgacacgtc gggcacgtgg
     1021 tcgaccgcgg ccgtgctcga cgacaccgtg atctggccga cgcgtccggg cacgatctac
     1081 ggcatcgacc gggcgtcggg ggcggtccgc tggacgctcg acctgccgcc accgctgatg
     1141 ggttcgccag tcgtcgggga cggtgtgtgg atccaaggcg actgcaatgg cgtgttgcac
     1201 ggtttcgacg tgcacgaacc gcatgtgcaa ccgcgcgaac tgtggtcggt cgaactgggt
     1261 gggtgcatcg agtcgacacc ggcggtctgg aagggccgca tcttcgtcgg gacgcgggcc
     1321 ggattggagt acgcgctcga ttagcctttg agaatgttga tcgcggccgc gctcatcgtg
     1381 atggggatcc tggagatcta cgtgatcgtc caggtcggcc aggcgatcgg cgccttgtac
     1441 acgatcgcat tgctcatcct catgggcatc ctcggcgtgt ggctggccaa gcgcgaaggc
     1501 ttcgccgtgt tccgccgctt ccgccggacg acatccgaag gacgagtgcc gacatcggaa
     1561 gtgatcgacg gcttcctcat cttcaccggc gccgtgctca tgatcatccc gggcttcatc
     1621 agcgacgtca tcggcatctt gttgttgatc cccccgacgc gcgcgatccc gcgcaacttc
     1681 atcaagcggc ggttccgcat cacgatcatc gaccagtacg gcccgcaagg ccgcggcccc
     1741 cgcccggtac cccccgacga catcatcgac atctgacggt cgggcgtttt tctgcagcga
     1801 atttcgccgc agaaagcgcc cgagcgaaac agttagagct cgccggcggc gaggagctcc
     1861 acggggtcgc ggacggcgac ggtgccgagg taaccgccgc ccatgtgcac cgcgtcgtgc
     1921 gcggcgtacc cgagcagcgc ctgcgactcg ggcggcaggg tgcgcacgaa gtcgagcggc
     1981 gtcgcgaggt actggttctc ctcggtgcgc agccagccga cgcagtagct gacgtgcatg
     2041 ccgcgccgga aggtgccggt ggtgacgttg gcgccgccgc cgtgcaaagt cgagccgagg
     2101 tagacgatcg cggagccggc gcgcatctcg gcggcttcga tctcgtccgg cgtcggggag
     2161 cgatcgtccg tccacttgtg gctgccgggg acgacgcggg tcgcgccgtt gtcggcggtg
     2221 aagtcgacaa gcgcgatcac cgacgccaac tggatctcgg ggtgcggctt gggcaggtgc
     2281 acccacacca actcgtcacg gtgcatgaac tggtcgtcgg agcccgggcc gcggtcgagc
     2341 acgtgcgcga tgttgagctg gtaggtcgcg caatgcggca ggaggatcgc gtcgcagacg
     2401 gccttgtaca ccgggtgcgt gaggatctcg gtggagaaca cgcgcgaacg accggcgaca
     2461 cccgtgacgt cacgagtctg cgcgccgaag aagtattgga tggcgggatt gatgaacggg
     2521 cgttccttcg ccggcttcgc cgcttcgagg aacgggtcga tctcggcgtt gaagcgcgcg
     2581 agcacgtcgg cgtcgagcaa gtcgtcgacg atgaccgcgc cgtcgcgttc cagcgcgccg
     2641 acgatctgtt cgggaggtgc cgaggccgcg agccgcgaga gagccatccc ggaacgatac
     2701 gacgctcagt cgagggggcg cagcagctcg gggacgatgg ggcagtcgat gcgcgccgcg
     2761 gcatccgtcg gcagctcgac accacgaggc cccgcgatgc ggtcgatgcg ctcgagactc
     2821 acgacgtcgg gcgctgcttc ttcggggtcg agcatctcgt cgacgtgctc ggccgcggtg
     2881 cgcgccgcgt cgaacaaccg ggccggaagg acgcgaccga cgccgcccac caaccacacg
     2941 tcgcgggccc ggccgccgag gtcgcgcaac agcgcgtcgg tgccggccgg cacgagcgtg
     3001 cggtcgggtc cgatcgcgaa cgcggggacg agcaacagcg cgagcgcctc gatgtcccag
     3061 acgtcgacga cgggcacgtt gcgatcggtc tggcgataac gcaggtcgcc gaacgcgtcg
     3121 acgccctcga cccgcacgga gaggaccgcg atgtcttggc gttcggcgag cgcctcgtcg
     3181 accgcgtggg gccagccgat cgtggcgacg agatcaccgt cgtcgagcaa cgggagcgtg
     3241 gccgcgagcc ggttcgcggt gcggtcggcg tcgagcgacg ccatcgcttc gcgcgccgcg
     3301 acgttcggtt cgggtgcggc caggatccgc gcgcacaccc accacagcgc gccgtgcgcc
     3361 cggtggtgtg cgagcacccg gcggcaggcg acgacgaggc tcgcgggctc ggacgcgaag
     3421 ccggccagcg cgcccgcggc ctcggcggcc agcacgtgat cggcgacacc gcggcgggtg
     3481 acggagcgca gccgctcggc cggtgtcacg tcccaggtca cccggcgaca ctaccgagca
     3541 ggcgcgtacg tcgtgcccgc gcgaactgcg gcgaacacgt cgtcgagggc ggcgagtgcg
     3601 ctcgcacccg cgcccagcgc gccgatgctg ccgcgcaccg tcaggcatcc gtcggccaac
     3661 gcctgctgcg cgttggtctc gccccgcgcc agcatgactg ccgtttcgta gcccgtgacg
     3721 aacgtgacat cacccggcgg catggccgcg cccacctcga cgcgcaatgc ctcattggtg
     3781 acgacaaggc agtaccgcac ctcgccccgg ccgggcacgc cggtcaccac gttctcgatc
     3841 acgaggcgat cgacaccggc cgcgccgggt gcctgcgcgg cggccgcggc catctcggcc
     3901 agccaatcct ctccgaggaa gctcgacacg ggggcaggct aggggcctga gtagcctcgg
     3961 ttcgatgtct cgccgggaag ccacgtttcc tcccgcactg gtgacggtgg ccgaccgggc
     4021 cgaggcgcgc atcctcgact tgctcgaccg cgaggtcgac cggtggcgcg gcgtcgacgc
     4081 cgcgctcgtc gcgccgctcg acgcgctgcg cgagttcgtc gtcgccggcg gcaaacggct
     4141 gcggcccgcg ttctgtcact gcgcgttcgt cggcgcgggc ggcgcacccg acgaccccgc
     4201 ggtgatcgac gccgcggccg cgctcgagct catccacacg ttcgcgctcg tccacgacga
     4261 cgtcatggac ggctccgaca cccgccgggg tcaagacgcg gtgcatcgtt cctttgcccg
     4321 ccgccaccgc gacgggtcgt ggcggggcga ggcgcgccgc ttcggcgacg ggatggccat
     4381 cctcgtcggc gacttcgcgc acgtctacgc cgacatgctc atgcgttcgg cactgatgcg
     4441 aggcacaccg cccgcggcga tcgaggtcta cgacacgctg cgcgtcgaac tgtgcgtcgg
     4501 ccagtcgctc gacctcatca cgaccgcgcg ctcgggcacc gacggcgaag cagctcgccg
     4561 catcgctacc tacaagtccg cgaagtacac cgtcgagcga ccgatgcacc tcggtgccgc
     4621 gctcgccggc cgcctcgacg agctgtccga tccgctcagc gcgatcgggc tcccgctcgg
     4681 tgccgcgttc cagctgcgcg acgacgtgct cggcgcattc ggcgacagcg acgtcaccgg
     4741 caagcccgtc ggcgacgact tgcgcgaagg caaggcgacg ccgctcgtcg cgctcgcggc
     4801 cgaacgcgcg gagcccgccg acgcgccctt gttcgcgaga ctcggcgcgg tcgacttgca
     4861 acccggcgaa gtcgcggcac tccaagacct cttcgtacgc accggtgcgt tgaaggacat
     4921 cgagcgcgag atcgaacagc ttgtcgacgt cgcgcgcact gcgatcgacg ccgcaccgtt
     4981 gtcgccggaa gcgcgcgagc ggctgtcgga gctcgcggac tacgtcgcct ggcgggatcg
     5041 atagatgccg ggagagatgc acgacgacga accggcgatc gacgtcgtcg acctcgagaa
     5101 gcgctacggc acagtgcagg cggtcgccgg tctgtcgttc agcgtgtcgc gcggggaggt
     5161 gttcggtctg ctcggcccga acggcgcggg caagacgacg acggtcgaga tcctcgaggg
     5221 ctatcgccgc cccgatggcg gtcacgcgcg cgtgctgggg ctcgacccgg tcacggacgg
     5281 cgcccagctg cggccgcgca tcggcgtcat gctccaggac agcggcctct atccgggcct
     5341 gcgtccgctc gaactgctgc gcttgttcgc ggcctattac gacaacccgc agtcgcccga
     5401 ggcgctcctc gaactggtcg ggctcgcgga gtcgacgggg acgacggtgc gccggctctc
     5461 gggcgggcag gcccagcgcc tgtcgctcgc gtgcgcactg atcggccgcc ccgacgtcgt
     5521 cttcctcgac gagccgaccg ccggcatgga tccccacgca cgggcgacga cgtgggagct
     5581 ggtgcgctca ctggccgcga acggcacgac cgtgatgctg acgacgcacg ggatggacga
     5641 agccgaacag ctgtgcgacc gggtcgcgat catcaccggc ggccggctcg cggccctggg
     5701 gacgcccgcc gagctgaccc ggcacgcggc cgccgacgag ttgtggttcg cggcggtcgc
     5761 cgcgctcgac gtccgcctgc tcgagaaggc gctcggcgtc gcgcccgaca cggtggtcga
     5821 agagcgcgcc ggggagtacc tcgtgcgcgc ggcggcgaca cccgagctga tcgccgacct
     5881 cgcgtgcttc ctgcgcgacc agggcgtcgt gctcggtgca ctgcaggcgg gccggcgcag
     5941 cctcgaagag gtgttcctgc agatcacggc ggaacgggaa ccgcgatgag cacgcgtgcg
     6001 atatccgcgc aggcgcgcgc cgagttcgtg ctgcagctgc ggcggggcga gaacgcgatc
     6061 gtcacgctcg cgatcccgct cggcgtcctg atcttcttca gcaaggtgga cgccgtctcg
     6121 accaacctcg acgatcccgt cgacttcctg gtgcccggtg tcctctcgct cggcgtcatg
     6181 gctgccgcga tggtctcact cgggatcgcg accgggttcg agcgccgcta cggcgtcttg
     6241 aagcggctcg gttcgacacc gctgtcacgt ttcgggctgc tgaccgccaa gaccgcgacc
     6301 gtcctcgcgc tcgaagtgat ccagctcgtg ctcgtcgtcg tcaccggcat cgcgctcgga
     6361 tgggacgtga acggcggcgt cgtgcccgcc ctcggcctgc tgctgctcgg cacggtcgcg
     6421 ttcgccggca tcgggttgtt gatggccggc acgttgcgcg cggaggccaa cctcgcgctg
     6481 tcgaacgggc tgttcctcgt gctgctgttc ctcggcggca tggcgtatcc gctcgacaag
     6541 ctgcccggcg cgctcgaagc gttcgcgaag ctgttgccgg ccgccgcgct ctccgagacg
     6601 gtccgttcgg tgctgtcgtc ggaaccgttc cccggaggcg agctggtcgt gctcgtcgtg
     6661 tgggcgatcg cggcgccgct cgcggcggct cgttggttcc gctgggagga atgaacgggc
     6721 tgcgccgcac ggggccaatc cgcgcacgct ctacgggggc gcgggggcgg cgtggtcgac
     6781 gagctggtcc agctcgtctt gcgacggcgc gccgaaccgg cgggcgcgga tcgtgccgcc
     6841 ggcgtcgatg aagaacgtct gcgggatcgc gcgcacgccg tacatgctgg cgacgtcgtc
     6901 gtcctcgtcg tgcaacatcg gaaacgtgag cccgagctcg tcgacgaagt cgcgggagtc
     6961 gccgatcagg tcgcggtacg agacgccgac gatcgccacg tcgtcgcccc gctcgtcgcg
     7021 gacgtgctgg agccggggca gctcctgcga gcagtcgtag caccacgacg catagaacgt
     7081 gagcacgacg ggagtgccgc gtagctgcgc gagcgagacg acttcgcccc cgagtgaggg
     7141 gagcgtgaag tcgggagcca tcgatccgac gtcggccttc ccggcgacca tcgcgcccgc
     7201 cggcatcgtc atgctcgtgg tggccgcggc atcgttgtcg tcgtcgcggt tggcgacgag
     7261 gatcacgctc agcagcgcga gcggcaccag caccgcgaca gcaacgacag cggcgacccg
     7321 gccgcgaccc atggaggact agccgaccag cgcgtcgacc gcgaccgcga cgaacacgac
     7381 catgaggtag atgttcgaga acgcgaagaa gcggatggcg cgcttgggcg tcgggtcacg
     7441 gcgcagcgcg agcgcctgga gcacgaacgc ggcgccgatg gcggcggcgg tgagtgcgta
     7501 gaccaaaccc acccagccgg tgagtaccaa cgcgagcgag gccgcgacgg tcaccatcgc
     7561 gtagccgcag atctgcttgg ccgtcatgtc gacgcccgcg accacgggca gcatcgggat
     7621 gcccgccgcc gcgtagtcgt cgcggtactt gagggcgagc gcccagaagt gcgcgggcgt
     7681 ccacaggaac acgacgagga agagaaccca cgccggcgcc gcgaggtcgt tgcgcaccgc
     7741 ggcccacccg acgagcgcgg gcaccgcgcc cgccgcgccg ccgatcacga tgttctggac
     7801 cgtgcgcggc ttcagccaga tggtgtacac gaacacgtag aacagcgtcg ccgacagcgt
     7861 cagacacgcc gccaacaggt tggcgaccgt cgccagcagc gcgaacgcga tgacgttgag
     7921 cacgatgccg aacacgagcg cgtgcgtcgg attgagctca cccatgggga gcggccggcc
     7981 gtgcgtgcgc cgcatgagct ggtcgcggtc acgctcgatc cagcagttga tcgtgttggc
     8041 gccaccggcc gcgagcgcgc cgccgacgac gacggcaagg atcagtgacc actccggcag
     8101 gccaccctcg gcgaggatca tcggcgggac cgcggtgacg agcagcagct cgatgacccg
     8161 cggcttcgtg agccggaagt acgcgccgat cgtcgcggcc aggggccggc gggactcgac
     8221 gtcgagacgg agagcgggct gcaccatggg cggggtcgtc gacgacgtta cgtcacttga
     8281 cgacgagcgt tcctttcatg ccctgtccct tgtggccggg gatatcgcag tagtactcgt
     8341 actcgccggg ctgcagctcg aacgacgcag agtcgctctt ctccgagggg ctgaccgcgg
     8401 ctttcccgtc gacaccctcg atgacgaacg tgtgctgttg cttaccctct tcgagcagcg
     8461 tgacctcgag cgtgccggcg tcggcattga tcttgccgat gtcgtacttg atgtcgtggg
     8521 cctcgacggt gaccttgccg cccgtcgcgt cgacggtctt gccgcctccg ccgcccccgc
     8581 cgcccccgca gcccgccggc acgctcgcgg cggccagcgt cgcgacgatg agaaggggac
     8641 gcgaaaagcg gcgcattacg gactcctggg acggctccgc agcggagcgg tacgggcgga
     8701 gcgtaccggc cggggaggct gcttccggga atcgatggtt atcctccggc ttcgtgagca
     8761 gcgccggtga gaacgacgag gacgagaagc agtcgatccg gatgacggcc gaccggctgc
     8821 tcgtcacgcc gtcgacggag gccggcgagc gcaagtcccg cgccggcatc ctcatcccgg
     8881 ccaccgccga ccagagccgt cgcctcttct ggggcgaagt ggtcgccgcg ggcccgacgg
     8941 tgcgcagcat cgagcagggc gaccgcgtgc tgttctcacc cgacagcggg ttcgaggtcg
     9001 agatccgggg cgacgagtac ctgatcctgc gcgagcgcga cgtccacgcg gtcgcgtcca
     9061 agcgcgacga aggacaggcg gggctgtacc tgtgatcgat cctgagagtg cgctgcgcat
     9121 cgacgggcag gccgtcctgg tgaccggcgg cacacgcggc atcgggcgcg cgatcgcgga
     9181 ggccgcgggc gccgccggcg cgtcggtcgc ggtgttggcc cgcaagcccg acgagctcga
     9241 ggagacggcc aaggcgatcg ccgccatcgg cgcaccggtc acgaccgtgc agggatccgt
     9301 cggcgacccc gagatgcccg agcgcgcggt cgcggcgatg ctcgacgcgt tcggtcgctg
     9361 cgacgccgtc gtcaacaacg cggccgtgaa ccccgcgttc ggtccgttga tggacgccga
     9421 catgggcgcg gtggccaagg tgtgggacgc gaacatcgcc ggcccgctgc gcttcgcacg
     9481 cgccgcctgg cacggctgga tgaaggacaa cggcggcaac atcgtcaacg tcgtctcggt
     9541 cggcggcatc cggcccggac cgttcatcgg cgcctacaac gtgtcgaagg ccgcgctcat
     9601 ccacctgacg ctgcagctcg cgcaagagct cgcccccacg gtccgcgtga acgcagtcgc
     9661 gcccgcgttg gtgaagaccg acatggcg
//
LOCUS       DASUGA010000540         2564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_90492, whole genome shotgun sequence.
ACCESSION   DASUGA010000540 DASUGA010000000
VERSION     DASUGA010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90492"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VFX21_10745"
     CDS             complement(<1..546)
                     /locus_tag="VFX21_10745"
                     /inference="COORDINATES: protein motif:HMM:NF039283.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HEX5096486.1"
                     /translation="MERAIADYALLADGSSAALVHRSGSIDWWCPARFDAESAFARLL
                     DERGGSWTIRPKDALGVTREYVANTMVLQTDFTTATGTLRLLDALALPRAEPHRLGRD
                     IVRRLVRRAECVEGAVDVEVELAPRFDYGREVPSFDDAEPALEIRGRAQRLRLHANRR
                     LRTSAGRAAAEFTLRAGDVANY"
     gene            complement(577..1425)
                     /locus_tag="VFX21_10750"
     CDS             complement(577..1425)
                     /locus_tag="VFX21_10750"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5096487.1"
                     /translation="MALLRKIVSRLTHWKGRPAHTEPYTPFPAPAMPRVRIAVAGDVG
                     KRGRHASAIAAAVARAGTERAYNVLLLLGDNVYPRGDPALLPTTVFEPYRPVLAAGAT
                     LLAILGNHDVMRGHGPEQLRRLGQAGPWWSHCVDGVLIVGLDSTRVDDATQRTWLEAT
                     LRDTDARWKIVAIHASPYSSGYQGSSRAVREAFVPLFERYGVRLVLSGHDHDYQRSHV
                     INGVTYVVAGGASTTRRAGRAPFTAVSFKWHHFVDVSVFDDHLLLRAINAELRVGDTY
                     ALSLDR"
     gene            complement(1435..2265)
                     /locus_tag="VFX21_10755"
     CDS             complement(1435..2265)
                     /locus_tag="VFX21_10755"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018160308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5096488.1"
                     /translation="MEIANVDMAKLWDGEDGDDWTDDADRYDATSRYVWPLFTANVAV
                     SPTDTLLDIGCGTGHSMRSYAPLVQHAVGIDLSRRMLECARERSAAAGHTNVEYLHAD
                     AQVHPFEPDTFGIAISSMGGMFFADPIAAYSNIRAALRPRGRLALLSWQPFVANEWLR
                     TFFDALACGRDLAPPPAGVPGPFGLAEPDGVRQMLGAAGFQDVDLMPLDAPVWLGATA
                     DDAWDFVSRMGIVRGLTGGLDDDTKRAALDRLRAAVAASETPDGVQLAAAEWLISAHT
                     "
     gene            2413..2486
                     /locus_tag="VFX21_10760"
     tRNA            2413..2486
                     /locus_tag="VFX21_10760"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2447..2449,aa:Arg,seq:tct)
BASE COUNT          397 a          876 c          910 g          381 t
ORIGIN      
        1 gtagttcgcg acgtcacccg ctcgcaaggt gaactcggcg gcggcgcgcc cggcactggt
       61 ccgcagccgc cgattggcgt gcaaccggag gcgctgtgcc cgcccgcgga tctcgagtgc
      121 cggctcggcg tcgtcgaacg acggcacctc gcgcccgtag tcgaagcggg gtgcgagctc
      181 gacttcgacg tcgaccgcac cctcgacaca ctcggcccgc cgcacgagcc ggcgcacgat
      241 gtcgcgtccc agccggtggg gttcggcgcg cgggagcgcg agcgcgtcga gcaaccgaag
      301 tgtgccggtt gcagtcgtga agtcggtttg caggaccatt gtgttggcga cgtactcgcg
      361 cgtgacaccg agcgcgtcct tgggtcggat cgtccacgaa ccgccgcgtt cgtcgagcaa
      421 ccgcgcaaac gcgctctccg cgtcgaaacg ggcgggacac caccaatcga tcgaacccga
      481 ccggtgtacg agtgccgcgc tcgaaccgtc cgcgagcagt gcgtagtcgg cgatcgcgcg
      541 ctccaccgcg ggtgtgtacc cagaccggtg cggccgtcac cggtcgagtg acaaggcgta
      601 cgtgtcccca acgcgcagtt ctgcgttgat ggcgcgcagc aggagatgat cgtcgaagac
      661 gctgacgtcc acgaagtgat gccacttgaa cgagaccgcc gtgaacggcg cgcgacccgc
      721 gcggcgggtc gtggacgcgc caccggcgac gacgtacgtc acgccgttga tgacatgact
      781 gcgctggtag tcatggtcgt ggccggagag cacgagccgc acgccgtagc gctcgaacag
      841 cggcacgaac gcttcccgga ctgcgcggct cgaaccctgg tatccggacg agtacggcga
      901 ggcgtggatc gcgacgatct tccagcgcgc gtcggtgtct cgcagcgtgg cctccagcca
      961 tgtgcgttgt gtcgcgtcgt cgacgcgagt cgaatcgagc ccgacgatca ggacgccgtc
     1021 gacgcagtgc gaccaccacg gccccgcttg gcccagccgc cgcagttgtt cgggaccgtg
     1081 cccgcgcatg acgtcgtggt tgccgaggat cgcgagcagc gtggcaccgg ccgcgagcac
     1141 gggacggtac ggctcgaaca cggtggtcgg cagcagcgcg ggatcacctc tcggatacac
     1201 gttgtcgccg agcaacaaca gcacgttgta ggcacgctcg gttccggcgc gggcaacggc
     1261 ggccgcgatc gccgatgcgt ggcgaccacg cttgccgacg tcacccgcga ccgcgatccg
     1321 cacgcgcggc atcgccgggg ccgggaacgg cgtgtacggc tcggtgtgtg cgggccggcc
     1381 cttccagtga gtcagccgcg agacgatctt gcgcaacagg gccacggggg gagttcaggt
     1441 gtgcgcgctg atcagccact cggctgcggc gagttgcacg ccgtcgggtg tctcgctcgc
     1501 cgcgactgcc gcccgcaacc ggtcgagggc ggcgcgcttg gtgtcgtcgt cgagaccgcc
     1561 cgtaaggccg cgcacgatgc ccatccgcga cacgaagtcc cacgcgtcgt cggcggttgc
     1621 gcccagccac accggggcgt cgagcggcat gaggtcgacg tcctggaagc cggcggcgcc
     1681 gagcatctgc cgcacgccgt cgggttcggc gagaccgaac ggtcccggca cccccgccgg
     1741 cggcggcgcc aggtcacgac cgcacgccag cgcgtcgaag aaggtccgca gccactcgtt
     1801 ggcgacgaac ggctgccacg acaagagcgc gagccggccc cggggccgca gcgcggcgcg
     1861 aatgttcgag tacgcggcaa tggggtccgc gaagaacatg ccacccatgg agctgatcgc
     1921 gatgccgaac gtatccggct cgaaggggtg cacctgcgcg tctgcgtgga ggtattcgac
     1981 gttggtgtgg ccggcggccg cgctgcgttc tcgcgcgcac tcgagcatgc gccgtgagag
     2041 gtcgatgccg accgcgtgtt gcaccagcgg cgcgtagctc cgcatggagt gcccggtgcc
     2101 gcaaccgatg tcgagcagcg tgtccgtcgg tgagaccgcg acgttcgcgg tgaagagcgg
     2161 ccacacgtat ctgctggtcg cgtcgtagcg gtcggcgtcg tcggtccagt cgtcgccgtc
     2221 ttcgccgtcc catagtttcg ccatgtcgac gttcgcgatc tccatgggtc ctcctcactg
     2281 cagcaccgtc gatgcgcgac cggctcgttc ggctccacgt caatactgct gcgagcgcgc
     2341 tcggattgcg aaggcccgcg aggacggcgc cggcgggccg gtaccatccg cgagggccgg
     2401 cgagcggctc acgcctccgt agctcagttg gatagagcaa gtcacttcta atgactaggt
     2461 cgcaggttcg agtcctgccg ggggcgccgg tactgcacag gggcggcgca aggcgacgtt
     2521 cgctcccgct acccacccga gacgtcgaag gcagcctctc gcac
//
LOCUS       DASUGA010000541         2872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1002678, whole genome shotgun sequence.
ACCESSION   DASUGA010000541 DASUGA010000000
VERSION     DASUGA010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002678"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1704
                     /locus_tag="VFX21_10765"
     CDS             <1..1704
                     /locus_tag="VFX21_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096489.1"
                     /translation="PDCTACTQPQFWASVAGPDAAKGGGNAALSDYCEYKDPTTQLQS
                     GIASVPSVADNCPPGSNNHSEDTEEAQYFVIDKQSGSAGNLRVDLYDPAWINTVSSTS
                     PCGSDATKAVWQMFESSSPTSLRRPSSPPGPGGLPPADIPVSCSGDQLSRHRHGGDAL
                     FNASLPDNFSPDRPPEYCAWVTGQNVPDGVLSAGEPCEPEKNAPWGPLGPETNSFPTA
                     IPPDPNYKGDGRINNLFRQYLLDTRDTSGVTTTNPRLSLSGATTYELYPPDDTPTNPY
                     DNEDHASAACADSYNGHANPWLAHEAKVNGIDTSYDTYHAWTRLLCSGGTSADGRWVL
                     KVYGTPGSIAVNSFAIAASSNGARDGGSDPMVSVNAVDRLATYTNKPTASGDFYLARV
                     VPSSVPRTLRLTLFDTGDFNCPTDTGANCPNTAEVQIQIKPANDDPLNPFKTTAGPDP
                     GAPQLNELDGCQYTQPAADDWHNGLTAPWEIGTKDYGTLGDTEDDCKILHVKRKEWNG
                     RYVMVEIPIPDNSGYYCDASEVRSCWLKIYYTSNDGTMAITDVSTWVATFSGNPVRIV
                     K"
     gene            1727..2641
                     /locus_tag="VFX21_10770"
     CDS             1727..2641
                     /locus_tag="VFX21_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013015416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX5096490.1"
                     /translation="MPDASAVEIREVGPRDGLQNEAPVAIEARVRLIDALSRTGLRRI
                     EAASFVRDDAIPPMAGSDQVMAQIARRPGVVYSALVPNPKGAERALAAQADELELVVS
                     ASETHNRKNVRRTVAESLVAAHELIELAHGAEVPVEAIVSTAFGCPYEGDVAPERVAQ
                     LAGHLLDAGADRLSFGDTTGMATPRRVDDLLDALSRARIEPERVGLHFHNTRGTALAN
                     VLAGLECGVTRYDASIGGLGGCPYAPGASGNAVTEDLVHMLEDMDIDTGIDLDALLDC
                     AHLAQDIVGRELPSALLHAGPRLRKYEA"
     gene            2674..>2872
                     /locus_tag="VFX21_10775"
     CDS             2674..>2872
                     /locus_tag="VFX21_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX5096491.1"
                     /translation="MSNDRDDRVRDHTERALQGNLAKEGEKLARQHKLFVRDRLARLL
                     DDGSFVEDGLLANALAGDLPAD"
BASE COUNT          514 a         1017 c          955 g          386 t
ORIGIN      
        1 cctgactgca ccgcgtgcac ccagccgcag ttctgggcga gtgtcgccgg acccgacgcg
       61 gccaagggcg gcggcaacgc ggcgttgagc gactactgcg agtacaagga cccgacgacg
      121 cagctgcagt cgggcattgc gagtgttccg agcgttgccg acaactgccc ccccggcagc
      181 aacaaccaca gcgaggacac cgaggaagcg cagtacttcg tcatcgacaa gcagtcgggc
      241 tcggccggga acttgcgtgt cgacctctac gacccggcgt ggatcaacac ggtctcgtcg
      301 acgagcccgt gcggcagcga tgcgaccaag gccgtgtggc agatgttcga gagcagctcg
      361 cccacgagtc tgcgccggcc gagctcaccg ccgggcccgg gtggccttcc gccggcggac
      421 ataccggtat cctgctcggg cgaccagctc tcccgccacc gccacggtgg tgacgcgctg
      481 ttcaacgcca gcctgcccga caacttcagt cccgaccggc cgcccgaata ctgcgcgtgg
      541 gtcaccggtc agaacgtgcc cgacggtgtg ctgtcagccg gcgaaccctg cgaaccggag
      601 aagaacgcgc cctggggccc gctcgggccg gagacgaaca gcttcccgac cgcgattccg
      661 ccggacccga actacaaggg tgacgggcga atcaacaacc tgttccgtca gtacctcctc
      721 gacacgcgcg acacgagcgg tgtcaccacg accaatcctc ggctgagcct gtccggcgcg
      781 acgacgtacg agttgtaccc gccggacgac acgccgacga acccctacga caacgaggac
      841 cacgcgtccg ccgcgtgcgc ggacagctac aacggtcacg cgaacccgtg gttggcccac
      901 gaggcgaagg tcaacgggat cgacaccagc tacgacacct accacgcgtg gacccggctg
      961 ctgtgctccg gcggtacgag cgccgacggt cgctgggtcc tcaaggtcta cggcacgccc
     1021 ggctcgatcg cggtcaactc gttcgcgatc gcggcgagct cgaacggcgc ccgcgacggt
     1081 ggcagcgacc cgatggtctc cgtgaacgcg gtcgaccggc tcgcgacgta caccaacaag
     1141 ccgaccgcct ccggcgactt ctacctcgcg cgcgtcgtgc ccagctcggt gccccgcaca
     1201 ctgcggctca cgctgttcga cacgggtgac ttcaactgcc ccaccgacac gggcgcgaac
     1261 tgtcccaaca ccgccgaagt ccagatccag atcaagcccg ccaacgacga cccgttgaac
     1321 ccgttcaaga cgacagcggg ccccgacccc ggcgccccgc agctgaacga gctcgacggc
     1381 tgccagtaca cgcagccggc cgcggacgat tggcacaacg gcttgaccgc gccgtgggag
     1441 atcggcacga aggattacgg caccctcggc gacaccgaag acgactgcaa gatcctccac
     1501 gtcaagcgca aggagtggaa cggtcgctac gtgatggtcg agatcccgat ccccgacaac
     1561 tccggctact actgcgacgc gagcgaggtc cggtcgtgct ggttgaagat ctactacacg
     1621 tccaacgacg gcacgatggc gatcaccgac gtgagcacgt gggtcgccac gttcagcggg
     1681 aacccggtcc ggatcgtcaa gtagcgcggt cgcgtatcct gcctgcgtgc cggatgcgtc
     1741 cgcggtcgag attcgcgaag tcgggccgcg tgacggtctg cagaacgagg cgccggtcgc
     1801 gatcgaggcg cgggtccgcc tgatcgacgc gctgtcacgc acggggctgc gccgcatcga
     1861 agccgcgtcg ttcgtgcgcg acgacgcgat cccgccgatg gccggctccg atcaggtgat
     1921 ggcacagatc gcgcggcggc ccggtgtcgt gtactccgcg ctggtgccga accccaaggg
     1981 cgccgaacgt gcgctcgccg cgcaggccga cgagctcgag ttggtcgtga gcgcgagcga
     2041 gacgcacaac cggaagaacg tgcgccgcac ggtggcggag tcgctcgtcg ccgcccacga
     2101 gctgatcgag ctggcgcacg gtgcagaggt accggtcgaa gcgatcgtgt cgaccgcgtt
     2161 cggctgcccg tacgaaggcg acgtcgcacc cgagcgcgtc gcgcagctcg cgggccacct
     2221 gctcgatgcg ggcgccgacc ggctgtcgtt cggtgacacc acgggcatgg cgacgccgcg
     2281 gcgggtcgac gacctgctcg acgcgttgtc acgggcgcgc atcgaaccgg aacgggtcgg
     2341 cctgcacttc cacaacacgc gcggcaccgc gctcgcgaac gtgctcgccg gcctcgagtg
     2401 cggtgtgaca cgttacgacg cgtcgatcgg cggcctcggc ggctgtccgt acgcgccggg
     2461 cgcgtcgggg aacgccgtca ccgaagacct ggtgcacatg ctcgaggaca tggacatcga
     2521 cacgggcatc gacctcgacg cgctgctcga ctgcgcgcac ctcgcccaag acatcgtcgg
     2581 gcgggagctg ccgagcgcgc tcttgcacgc gggcccgcgg ctgcggaagt acgaggcatg
     2641 atcttgcatg agtaacgaat atgagtaacg aatatgagta acgaccgcga cgaccgcgtc
     2701 cgcgaccaca ccgagcgggc gctccagggc aatctcgcca aggagggcga gaagctcgcg
     2761 cgccagcaca agctgttcgt gcgcgaccgc ctcgcgcggc tgctcgacga cggctcgttc
     2821 gtcgaagacg gcctgctcgc gaacgcgctc gccggcgact tgccggcgga cg
//
LOCUS       DASUGA010000542         2436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1515440, whole genome shotgun sequence.
ACCESSION   DASUGA010000542 DASUGA010000000
VERSION     DASUGA010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515440"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..799)
                     /locus_tag="VFX21_10780"
     CDS             complement(17..799)
                     /locus_tag="VFX21_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5096492.1"
                     /translation="MVAEHATGWPALTARGLHKTFGSKVAVEHVDLDVMPGSFFGIVG
                     PNGAGKTTTLRMATGLLRPDRGQVWVDGVDVWADPVLAKRMIGVLPEDLALFERLSGR
                     ELLTFHGALRNMDPRVVIERSEELLDLLGLSDAADALVVDYSHGMRKKLVLAAALLHG
                     PRLLFLDEPFEAIDPVSARAIRNLLAHFTSTGSTIVFSSHVMELVERLCDHVAVLARG
                     TILWSGPLDELRGHRSLEDAFVELVGEPSGRQELSWLGNSPA"
     gene            complement(822..>2436)
                     /locus_tag="VFX21_10785"
     CDS             complement(822..>2436)
                     /locus_tag="VFX21_10785"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX5096493.1"
                     /translation="RRARDRADRLQKFAASAARAASVDAVVRAIATDGVAAADGYVVN
                     IAMRVEHDSPSQFVRTRRLHPGDSITWDPVPPDANDSLITALGSGRPGFYRTRAAYEA
                     QFPGSDKVLRLNDARAVACLPLLDSDGHVIGAIGYTFRAEQAFNDDQRVLLETVADVA
                     AQSLDRARLYEQERDVAGALQRALLPSALPELDGVETEARYVAGGRGVSVGGDWYDVL
                     RFVDGRVGLLIGDAAGRGVEAATLMGKVRHAAAALAMDHESPAVVLARVNEFLHTIST
                     RRDMVTCCYFVLDHDRGILRYSSAGHPPALLLENGRAPVFLEGARGVPLGVIPTAAYP
                     EATYALNDAATLVLYTDGLIERRGETIDVGLERLVATTEGHDDDINALCDRLTTTLLG
                     DNSEDDVALLVARVCAVPARGRLDLELPADSRRLHELRARVTRWLTDAGVAPAVIPEV
                     VIALNEAASNSMVHAYAGTAERGHVKVSLALAESALTATVSDEGNWRDKGPLHDGRGL
                     EMMHALMTEVEVERDANGTRVKLTREMVG"
BASE COUNT          391 a          828 c          834 g          383 t
ORIGIN      
        1 cggatcaggc gcagtttcag gcgggcgaat tgccgagcca cgaaagttcc tgccgcccgc
       61 tcggttcacc gaccagctcg acgaatgcgt cttccaggct gcggtggccc cgaagctcgt
      121 cgagcggacc cgaccacaag atggtgccgc gcgcgagcac cgcgacgtga tcgcacagcc
      181 gctcgaccaa ctccatgacg tggctcgaaa acacgatcgt cgaacctgtc gacgtgaagt
      241 gtgccagcag attgcgaatg gcgcgggccg agaccgggtc gatcgcttcg aacggctcgt
      301 cgaggaacaa caagcgcggg ccgtgcagga gcgcagccgc cagtaccagc ttcttgcgca
      361 tgccgtggct gtagtcgacg accagcgcgt cggcggcgtc gctcaggccg agcaggtcga
      421 gtagttcttc gctgcgctcg atcacgacac gcgggtccat gttccgcaag gcaccgtgga
      481 acgtgaggag ctcacgaccg ctcaatcgtt cgaacaacgc aaggtcttcg ggcaagacgc
      541 cgatcattcg cttcgcgagc acagggtcgg cccacacgtc gactccatcg acccacactt
      601 gtccgcggtc cggacgcaag aggcctgtcg ccatccgcaa cgtcgtcgtc ttgccggcgc
      661 cgttcggccc gacgatgccg aagaaggagc ccggcatcac atcgagatcg acgtgttcga
      721 ccgcgacctt cgagccgaac gtcttgtgca atccccgtgc tgtcagcgcc ggccacccgg
      781 tcgcgtgctc cgcaaccacc gcgcccaggg taggcgacga attagcccac catctcccgt
      841 gtcagtttca cgcgcgtccc gttggcgtca cgctccacct ctacctcagt catgagcgcg
      901 tgcatcatct ccaacccgcg cccgtcgtgc agcgggccct tgtcgcgcca gttgccctcg
      961 tcgctcacgg ttgcggtcag cgccgattca gccaacgcca agctcacctt cacgtgtccg
     1021 cgttccgcgg ttccggcgta ggcgtgcacc atgctgttcg atgcggcctc gttcagtgcg
     1081 atgacgacct cgggaatgac ggccggggcg acgcccgcat ccgtgagcca gcgcgtgacg
     1141 cgggcgcgga gttcgtgaag ccgccgggag tcggcgggga gttcgaggtc gagccggccg
     1201 cgagcaggga cggcgcacac gcgtgcgacg aggagcgcga cgtcgtcttc gctgttgtcg
     1261 ccgagcagcg tggtcgtgag gcggtcgcag agcgcattga tgtcgtcgtc gtgcccttcg
     1321 gtcgtcgcga ccagccgctc cagaccgacg tcgatggtct cgccgcgccg ttcgatgagt
     1381 ccgtcggtgt acagcacgag ggtggccgcg tcgttcaagg cgtacgtcgc ttccggatac
     1441 gccgcggtcg ggatgacgcc gagcggcacg ccgcgcgctc cctcgagaaa caccggcgca
     1501 cgaccgtttt cgagcagcag ggcgggcgga tggccggccg acgaatagcg caagatcccg
     1561 cggtcgtggt cgaggacgaa atagcagcac gtgaccatgt cgcggcgggt cgaaatcgta
     1621 tggaggaact cgttgacgcg ggccaggacg accgcgggcg actcgtgatc catggcgagc
     1681 gcggcggccg cgtgacgcac cttgcccatg agggttgcgg cctcgacgcc gcgtcccgct
     1741 gcgtcgccga tgagcaggcc gacgcggccg tcgacgaatc gcagcacgtc gtaccagtcg
     1801 cccccaacgg agaccccgcg cccgcccgcg acgtatcggg cctcggtttc gacgccgtcg
     1861 agttcgggca gcgcggacgg gagcaacgcg cgctggagcg cgccggcgac gtcgcgttcc
     1921 tgttcgtaga gacgcgcccg gtcgagcgat tgcgccgcga catcggcgac cgtttcgagc
     1981 agcacccgct ggtcgtcgtt gaacgcctgc tcggcgcgga atgtgtatcc gatcgcgccg
     2041 atgacgtggc catcggagtc gagcagcggc aagcacgcga ccgcgcgcgc gtcgttgaga
     2101 cggagaacct tgtcgctacc tgggaactgc gcctcgtacg cggcgcgcgt ccggtagaag
     2161 cccggtcggc cgctgcccag cgccgtgatc aaagagtcgt tcgcgtcggg tggaacgggg
     2221 tcccaggtga tgctgtcgcc gggatggagc cggcgcgtgc gcacgaactg cgacggcgaa
     2281 tcgtgttcga cgcgcatcgc gatgttgacg acgtaaccgt cggcggccgc gacgccgtcg
     2341 gtcgcgatcg ctcgcacgac tgcatccacg ctggcggccc gcgccgctga tgcggcgaac
     2401 ttttgcagcc ggtcggcgcg gtcgcgcgcg cgccgg
//
LOCUS       DASUGA010000543         3112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_72444, whole genome shotgun sequence.
ACCESSION   DASUGA010000543 DASUGA010000000
VERSION     DASUGA010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72444"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1485)
                     /locus_tag="VFX21_10790"
     CDS             complement(169..1485)
                     /locus_tag="VFX21_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096494.1"
                     /translation="MRVWKPVLRCFLVTALAAAALAIAPSAGSAATANPWPHTIVNGF
                     SNPAGDGGWLTYADGTVTTFGNAASFGDASGLALNGPIVGGAVAPDGLGYWLVARDGG
                     IFSYGSAHFHGSMGGTALNQPVFSMAPTPDGGGYWLVAFDGGIFSFGNAQFFGSMGGT
                     RLNQPITGITTGPGGNGYRMVARDGGIFSFGSAPFFGSAVDHDAHLTDVVGMAPSPTN
                     LGYWIARSNGTVYNFGDASALPAYSASTSNPVAAIFSNPTAQGYALVLADGTIVRRGV
                     APGGFDAGVHAVPSEIPVGTYLNRDELSCAWARMKTLNPVDGLNGDREGPGPQFATLT
                     SSDGAFFSTCSGWTPATSTAITSSPTASFGDGTFRVGTDVASGNWQAASPTARCYFER
                     LNAFTGEDDDIIATGVVADPIAPVDVTIAPGDAGFFSDGCGTWVKQ"
     gene            1593..1913
                     /locus_tag="VFX21_10795"
     CDS             1593..1913
                     /locus_tag="VFX21_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006357212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5096495.1"
                     /translation="MTALVADTFGALADPTRLAILERLGAGTASVSQLAAPHDMSLRG
                     VLKHIAVLEDAGLVRTTKRGRVRECELDRRRIDQAAAFIERLQTRWESRLDRLENYLE
                     TKKS"
     gene            1910..2353
                     /locus_tag="VFX21_10800"
     CDS             1910..2353
                     /locus_tag="VFX21_10800"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX5096496.1"
                     /translation="MSNDNSLRIERIIDAPADAVFGAWTTEEAMEQWYAEGDDPVVNV
                     VELDVRVGGRYHVEWGPRGAEPYVETGVYIEIDRPHRLVMRETLRGPGVDGWEDTTVT
                     VEFEDVEGKTRLVLEHVGFPSTEARDGARGGWPGFIDRLERYVTH"
     gene            complement(2359..>3112)
                     /locus_tag="VFX21_10805"
     CDS             complement(2359..>3112)
                     /locus_tag="VFX21_10805"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX5096497.1"
                     /translation="SAAFRMDPEVPLVVAEVNPEDMGNMPKGIASCPNCTTMIPVTAW
                     APLHRAARIDRLVVSSYQSVSGAGQVGLHELDAQWTKLDGRAADLSRAGSFAEPLVEG
                     EVWPKPIAGNVIPLAGSLKEEGYTSEEWKMVRETRKILHDDDIKCSVTCVRVPVYVGH
                     AVSANVRFHRPMSKAEAVELLRAAPGVQVVDDGDGIPTPQDAAGIDPVLVGRVREDIS
                     EPGTLNLWITGDNLRKGAALNAIQMAELLLAT"
BASE COUNT          503 a         1042 c         1090 g          477 t
ORIGIN      
        1 ggacgtgctc gtgctcggca tcggcacgct catcaccgcg ggtggcgtcg cgttgatcat
       61 caccctcggc gcggccgccg cccgcctcgt caagtcgatc ttttgacttc cgacccgggc
      121 gtcgctgcac ccgaatagtt caggtggagc gacgcccgag tcagtgagtt actgcttgac
      181 ccaggtgccg cagccgtcac tgaagaagcc ggcgtcgccc ggcgcgatgg tgacgtccac
      241 gggtgcgatc ggatccgcga caactccggt ggcgatgatg tcgtcgtctt cgccggtgaa
      301 cgcgttcagc cgttcgaagt agcagcgcgc ggtcggcgac gcggcctgcc agttgcccga
      361 cgcgacgtcg gtgccgacgc ggaaggtgcc gtcgccgaac gaagccgtcg gcgacgacgt
      421 gatcgcggtc gacgtcgccg gcgtccatcc ggagcaggtg ctgaagaacg caccgtcgct
      481 cgaggtgagc gtcgcgaact gcgggcccgg gccttcacgg tcgccgttca aaccgtcgac
      541 cgggttcagc gtcttcatgc gcgcccatgc gcacgagagc tcgtcgcggt tgaggtacgt
      601 gccgaccgga atctcggatg gcacggcgtg cacgccggcg tcgaagccac cgggcgcgac
      661 gccgcggcgc acgatggtgc cgtcggccag tacgagggcg tatccctgcg cggtcgggtt
      721 cgagaagatc gccgcgaccg gattgctggt cgacgcggag tacgcgggca gtgcgctcgc
      781 gtcaccgaag ttgtagacgg tgccgttcga acgcgcgatc cagtagccca ggttggtcgg
      841 cgacggggcc atgccgacga cgtcggtgag gtgcgcgtcg tggtcgacgg cgctgccgaa
      901 gaatggcgca ctgccgaagc tgaagatgcc gccgtcgcgg gcaaccatcc ggtagccgtt
      961 gccaccgggt ccggtcgtga tgccggtgat gggttggttg aggcgcgtgc cacccatcga
     1021 cccgaagaac tgcgcgttgc cgaagctgaa gatgccgccg tcgaacgcga cgagccagta
     1081 gccgccaccg tcgggtgtcg gcgccatcga gaagacgggc tggttcagcg cggtgccgcc
     1141 catcgatccg tggaagtgcg cgctgccgta ggagaagatg ccgccgtcgc gcgcgacgag
     1201 ccagtagccg aggccgtcgg gcgcgactgc gccgccgacg atcggaccgt tgagcgccaa
     1261 gcccgacgcg tcaccgaacg acgcggcatt gccgaacgtc gtgacggtgc cgtcggcgta
     1321 cgtgagccaa ccgccgtcac cggccgggtt ggagaatccg ttgacgatcg tgtgcggcca
     1381 ggggttcgcc gtcgcggccg acccggcgga cggggcgatc gcgagggccg ccgccgcgag
     1441 ggcggtcacg aggaaacacc ggagcaccgg cttccatacg cgcataaggg ggcctccatg
     1501 gtgggggaac aaagaggtgt tcccgcgcac gtcgagagcc acccgttgac acgacgcact
     1561 gtacggcgta atactgaacc agtgagttca ctatgacggc cctggtcgcc gacaccttcg
     1621 gggccctcgc cgaccccact cgcctggcga tcctcgagcg cctgggcgcc gggaccgcgt
     1681 cggtcagtca actggcggcc ccgcacgaca tgtcactgcg cggcgtgctg aagcacatcg
     1741 cagtgctgga ggacgccggc ctggtgcgca cgaccaagcg cggccgcgtg cgcgagtgcg
     1801 agctggaccg gcgccggatc gaccaggccg ccgcgttcat cgaacgcttg caaacccgtt
     1861 gggaatcgcg cctcgaccgg ctcgagaact acctggagac gaagaagtca tgagcaacga
     1921 caactcgctt cgcatcgaac gcatcatcga cgctccggcc gacgcggtat tcggcgcgtg
     1981 gaccaccgag gaagcgatgg agcagtggta cgccgagggc gacgatcccg tcgtcaacgt
     2041 cgtcgagctc gacgtgcgcg tcggcggccg ctaccacgtc gaatggggcc cgcgcggtgc
     2101 ggagccctat gtcgaaaccg gtgtgtacat cgagatcgat cggccgcacc gcctcgtcat
     2161 gcgcgagaca cttcgcggtc cgggcgtcga cggctgggaa gacacgacgg tgaccgtcga
     2221 gttcgaagac gtcgaaggca agacccggct cgtcctcgag cacgtcggct tcccgagcac
     2281 cgaagcacgt gacggtgcgc gcggtggctg gcccgggttc atcgaccgcc tcgagcgcta
     2341 tgtgactcac tagcgcgctc aggtcgcgag caggagctcc gccatctgga tcgcgttcag
     2401 ggcggcgccc ttgcgcaggt tgtcgccggt gatccacagg ttcagggtgc ccggctcgga
     2461 gatgtcttcg cgcacccggc cgacgagcac cgggtcgatc cctgccgcgt cctgcggcgt
     2521 cgggatgccg tcgccgtcgt cgaccacctg cacacccgga gccgcgcgca gcagctcgac
     2581 cgcctcggcc ttcgacatcg gccggtggaa ccgcacgttc gcggacaccg catgcccgac
     2641 gtagaccggg acgcgcacgc atgtcaccga gcacttgatg tcgtcgtcgt ggaggatctt
     2701 gcgcgtctcg cgcaccatct tccactcctc cgacgtatag ccctcttcct tgagcgaacc
     2761 ggcgagcggg atgacgttgc ctgcgatcgg cttgggccac acttcgcctt cgaccaacgg
     2821 ttccgcgaac gaaccggcgc ggctgagatc ggcggcgcgg ccgtcgagct tcgtccactg
     2881 cgcgtcgagc tcgtgcagac cgacctgacc ggcgcccgac accgattggt acgacgacac
     2941 gaccagacgg tctatgcgcg cggcgcggtg caacggcgcc catgcggtga cggggatcat
     3001 cgtcgtgcag ttcgggcacg acgcgatgcc cttcggcatg ttgcccatgt cttcggggtt
     3061 gacctcggcg acgacgagcg gcacttcggg atccatgcgg aacgcggccg ag
//
LOCUS       DASUGA010000544         2495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1567790, whole genome shotgun sequence.
ACCESSION   DASUGA010000544 DASUGA010000000
VERSION     DASUGA010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567790"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFX21_10810"
     CDS             complement(<1..272)
                     /locus_tag="VFX21_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096498.1"
                     /translation="MSAIEESQPTDASREREPDLRPSRGVAISDWLVRRTSSFLASRS
                     SRRGFLIGSAVTGSAVAAAGTTFATRPGTAYAAITGCPPGSLCDDG"
     gene            complement(269..793)
                     /locus_tag="VFX21_10815"
     CDS             complement(269..793)
                     /locus_tag="VFX21_10815"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEX5096499.1"
                     /translation="MSALVGPFAIAACLLAIAGVAKSAQPAATAGALAALRLPHQQWM
                     VRTGGLAEALLACAALVTGNAVLAILVALSYTGFALFVAAALSKGTPLSSCGCFGRID
                     TPPHPVHIVLDLLAAGAAIGVAAHGGTSIRAVLAAQPMGGVPFTLLLVIGVAAAALTM
                     TALPRTLAVARGRA"
     gene            complement(790..1728)
                     /locus_tag="VFX21_10820"
     CDS             complement(790..1728)
                     /locus_tag="VFX21_10820"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX5096500.1"
                     /translation="MARLTAHGFTLDVPAGWDARIYKRPAAGEVAATDTDGPPAPPGA
                     ATHAVVHVATIPMPPDMGDFGSAGLDQLGRDDAFIVVFDHGPEAVGKALFGREGMPRL
                     LEPGDFDSKTLQRALPGQSGVQSFFTESGRACCLYVVLGAHANSRRIVPRVNAVLADL
                     QIEPEVAGAQSATPPPTVLEAIAADPDLSEFARLLTKAGIADQLTGAGPLTVFAPTTT
                     ALRDATNLPAIEADSARLGRVVRFHVVADVVDIGPLAAQGPGASTTAATLEGHPITVT
                     IGAHEARVENVIIDPQRIVAGNGSVYTIAGLLEPPT"
     gene            complement(1716..>2495)
                     /locus_tag="VFX21_10825"
     CDS             complement(1716..>2495)
                     /locus_tag="VFX21_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096501.1"
                     /translation="VGLLVLLVAGLLRSHAEILRALHQLGVNLDPGASEVVAPSATAI
                     RSVPVTGGPPKSGRDVTGATPTGDAVSIAVRDVRHLTLLAFLSSGCATCLDFWSAFGT
                     EPAPEVPGDARLVIVTKGSAHESLSGVHRLAPSRIPVVMSSEAWDEYDVPVAPFFVLV
                     DGVSGAIVGEGAASNWGQVTSLMEQAVTDARITKRGRRSHAPGRPRSDALREARVDHD
                     LLAAGIVPGDARLYRLPEAESVPVHLDESVDDSPHERAWPD"
BASE COUNT          377 a          868 c          914 g          336 t
ORIGIN      
        1 tagccgtcgt cgcagagcga gcccggtgga cagccggtga tcgcggcgta ggcggtgccg
       61 ggtcgggtcg cgaaggtcgt ccctgcggcg gcgaccgccg agccggtgac ggccgagccg
      121 atcaagaagc cgcggcgact cgagcgcgac gcgaggaacg agctcgtgcg acgcaccaac
      181 cagtcggaga tcgcaacgcc acggctcggc cgcaggtccg gctcgcgctc tcgggacgcg
      241 tcggtgggtt gcgactcctc gatggcgctc atgcccgtcc tcgcgccacc gccagcgtgc
      301 ggggcagcgc cgtcatcgtg agcgcggccg ccgcaacgcc gatgaccaag agcaatgtga
      361 acggaactcc gcccatcggc tgagccgcga gcacggcacg gatcgacgtg cccccatggg
      421 cggcgactcc gatcgcggcg cccgccgcga ggaggtccag cacgatgtgc accgggtgcg
      481 gcggtgtgtc gatcctgccg aagcacccgc acgacgacag cggagtgccc ttcgacaacg
      541 cggcggcaac gaacagcgcg aagcccgtgt acgacaacgc gacgaggatc gcgagcacgg
      601 cgttgcccgt cacgagcgcg gcgcacgcga gcagcgcctc cgccagacct cccgtgcgca
      661 ccatccactg ctgatgcggc aagcggagcg ccgcgagcgc accggccgtc gcggccggct
      721 gcgcgctttt ggcgacgccg gcgatcgcga gcagacatgc ggcgatcgca aagggtccga
      781 cgagcgcgct cacgtcggtg gctccaggag cccggcgatc gtgtacacgc tgccgttgcc
      841 ggcaacgatg cgttgtgggt cgatgatcac gttctcgacc cgcgcctcgt gtgcgccgat
      901 cgtgacggtg atcgggtgac cctcgagcgt tgccgcggtc gtgctcgcac cgggaccttg
      961 cgcggcgagc ggtccgatgt cgacgacgtc ggcgaccacg tggaagcgca ccactcgccc
     1021 gaggcgcgca ctgtcggcct cgatggccgg caggttggtt gcgtcacgga gcgcagtggt
     1081 ggtcggcgcg aagaccgtca gcggcccggc tccggtgagc tggtcggcga tgcccgcctt
     1141 ggtgagcagg cgcgcgaact cggagaggtc ggggtcggcg gcgatcgcct cgagcacagt
     1201 cggtggcggc gttgcggact gcgccccggc gacttcgggc tcgatctgga ggtcggcgag
     1261 cacggcgttg actcgcggca cgatgcgccg actgttcgca tgggcgccga gcacgacgta
     1321 gaggcagcac gcacgacccg actccgtgaa gaacgattgg acccccgatt ggccgggaag
     1381 cgcgcgttgg agcgtcttcg agtcgaagtc gcccggctcg agcaggcgcg gcatcccctc
     1441 gcgcccgaac aacgccttgc caaccgcctc cggaccgtgg tcgaacacca cgatgaacgc
     1501 gtcgtcgcgg ccgagctggt cgaggcccgc gctcccgaag tcgcccatgt cgggcggcat
     1561 cgggatggtg gccacgtgca ccacggcgtg ggtcgcggcg ccgggtgggg ccggtgggcc
     1621 gtcggtgtcg gtcgccgcca cctcgcctgc cgccggacgc ttgtagatgc gcgcgtccca
     1681 accggccgga acgtcgagcg tgaacccgtg tgccgtcagt cgggccatgc gcgctcgtgc
     1741 gggctgtcgt cgaccgattc atcgaggtgc acgggcaccg attcggcttc cgggagccgg
     1801 tacagacgcg cgtcgccggg gacgatgccg gcggcgagga gatcgtggtc gacgcgcgct
     1861 tcgcgcagtg cgtcgcttcg gggacgaccc ggcgcatggg accgccgccc gcgcttcgtg
     1921 atccgggcgt cggtgacggc ctgctccatg agtgacgtga cctggcccca gttgctcgcc
     1981 gcgccttcac cgacgatcgc gcccgacact ccgtcgacca gcacgaagaa cggtgccacc
     2041 ggcacgtcgt attcgtccca tgcctcgctc gacatcacga ccggaatgcg cgacggagcg
     2101 agccgatgca cccccgagag gctctcgtgc gcggagccct tcgtgacgat gacgaggcgc
     2161 gcgtcgcctg gaacctcggg cgccggttcg gtgccgaagg ccgaccagaa gtcgagacac
     2221 gtcgcgcatc cgctcgacag gaacgcgagc agggtcaggt gtcgtacatc ccgtacggcg
     2281 atgctcacgg cgtcgccggt cggggtcgca ccggtcacgt cgcggcccga cttgggaggc
     2341 ccaccggtga cgggaaccga ccgaatcgcc gtcgcactcg gcgcaacgac ttcgctcgcg
     2401 cccggatcga ggttcacccc cagctggtgc agggcgcgca ggatctcggc gtggctgcgc
     2461 agcaagcccg cgacgaggag cacgaggagg cccac
//
LOCUS       DASUGA010000545         4051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_177120, whole genome shotgun sequence.
ACCESSION   DASUGA010000545 DASUGA010000000
VERSION     DASUGA010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177120"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFX21_10830"
     CDS             <1..189
                     /locus_tag="VFX21_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX5096502.1"
                     /translation="EVFNALIDKYPGMRGNLLGDDGGLHKFVNVYKDDDDIRYLDGLE
                     TAVGDGDVLSILPAVAGG"
     gene            202..1140
                     /locus_tag="VFX21_10835"
     CDS             202..1140
                     /locus_tag="VFX21_10835"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEX5096503.1"
                     /translation="MARYDDILGLIGNTPLVGVHALSPNPDVRIWAKLEGQNPGGSSK
                     DRIALKMVELAERDGRLQPGATILEPSSGNTGIGLALVAKLRGYHLRIVMPENVSIER
                     RQLLEIFGAEITLSPGAEGSNGAIRMAEKIAASEPGYVFLFQYGNPANPLAHYENTGP
                     EILRDCPEVDVFVAGLGTSGTLMGVGRYLKEHKPDVKIVAVEPPPGESAHGLRSLEDG
                     FVPPVFDREVLDRRMMVQTRDSIETNRRLLEECGVFSGISSGAAVAGAIKIAKTMDTG
                     TIVTLLPDGGWKYLSSGAWTDPVETVIERATKINYW"
     gene            1137..2039
                     /locus_tag="VFX21_10840"
     CDS             1137..2039
                     /locus_tag="VFX21_10840"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX5096504.1"
                     /translation="MSSISDAVAALRRGGLVAFPTETVYGLGADAANRDAVRRLYAVK
                     GRPPDHPVIAHIGRDATLDEYARDVPAVANVLARAFWPGPLTLILHRRPERVVAEVAG
                     GRGTVGLRVPDHDIALDLLDAFGGAIAAPSANRFGRVSPTTAAHVRADLGEDVDVVLD
                     GGATRVGVESTIVDCTTGGLTVLRVGAISAAQLAEVTGGDVPFGTGVAAPGTLASHYA
                     PRARVELVDRGDVDARLAAVRAEGLRAGVLDAPADVAEYARVLYARLRAADDDGLEVV
                     LAVLPPDDGGLGTVVRDRLRRAAG"
     gene            2125..2931
                     /gene="murI"
                     /locus_tag="VFX21_10845"
     CDS             2125..2931
                     /gene="murI"
                     /locus_tag="VFX21_10845"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX5096505.1"
                     /translation="MASDPRPIGVFDSGLGGLTVARALLDLVPGIPLVYFGDTGRFPY
                     GPKPGAVVRVYACEIADLLMARGVQMIVVACNSATAAALEDLRARVDVPVVGVIEPGV
                     RAARQATKNGRVGVIGTVGTISSGAYQRVAADVAPEVEVVCAACPGFVEFVEAGDVDS
                     DQVHVLAERLLAPVVAAKVDTLVLGCTHYPLLARTISDVMGPDVVLVSSADETAFEVR
                     RELNATTGADAMSRAATPMFMTSGEVDTFRTLGARFLGPEVEHVEAWSWS"
     gene            2922..3650
                     /locus_tag="VFX21_10850"
     CDS             2922..3650
                     /locus_tag="VFX21_10850"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5096506.1"
                     /translation="MELTVLGSSGSYGAPAGGACSGYLLRAGDVAIWMDCGNGTLVNL
                     QQHVNPAELTAVVITHGHPDHCVDIYGLHVMNKYGIDRAMLPVFAAEGVEKQLEHLVG
                     NWTDTFDWHTVGDGDRAQLDGVGLRFSRTDHPPPTTAVEISHEGKRLVYTSDTGPEWS
                     CEVFGAGADFVLSEATYQHDDIRAPIHLSAHQAGTAARDAHAKQLMLTHIWPTLDPVA
                     SVAEASEAYGGVVELAAPHRKIHI"
     gene            3676..>4051
                     /gene="rph"
                     /locus_tag="VFX21_10855"
     CDS             3676..>4051
                     /gene="rph"
                     /locus_tag="VFX21_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008284911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX5096507.1"
                     /translation="MATRASGRDPDELRGVTFTRDYTSMAMGSVLVEFGRTRVLCTAS
                     VEERIPPWLRNSGKGWVTAEYSMLPGSSAERVEREAAKGKQSGRTQEIQRLIGRSLRA
                     VTDLRAMPEVQITVDCDVLQADG"
BASE COUNT          617 a         1380 c         1426 g          628 t
ORIGIN      
        1 gaggtgttca acgccctgat cgacaagtac ccggggatgc gcggcaatct gctcggcgac
       61 gacggcggcc tccacaagtt cgtcaacgtc tacaaggacg acgacgacat ccggtatctc
      121 gacgggctcg agaccgcggt cggcgacggc gacgtgttgt cgatcctgcc ggcggtcgcc
      181 ggcggctgac ggcagctcgg cgtggcgcgc tacgacgaca tcctcgggtt gatcggcaac
      241 acacccctcg tcggtgtgca cgcgttgtct ccgaaccccg acgtccgcat ctgggccaag
      301 ctcgaaggcc agaaccccgg cggttcgtcc aaggaccgca tcgcgttgaa gatggtcgag
      361 ttggccgagc gcgacgggcg gctgcaaccg ggcgcgacga tccttgaacc gtcgtcgggc
      421 aacaccggca tcggcctcgc gctcgtcgcc aagctgcgcg gctaccacct gcgcatcgtg
      481 atgcccgaga acgtgtcgat cgaacggcgc cagctgctcg agatcttcgg cgcggagatc
      541 acgctgtctc ccggggcgga gggcagcaac ggcgcgatcc gcatggccga gaagatcgcg
      601 gcgtcggagc cggggtacgt gttcttgttc cagtacggca atcccgcgaa tccgctcgcg
      661 cactacgaga acaccgggcc ggagatcctg cgcgactgtc ccgaggtcga cgtgttcgtc
      721 gccgggctcg gcacgagcgg cacgctcatg ggtgtcgggc gctacctcaa ggagcacaag
      781 cccgacgtga agatcgtcgc ggtcgaacca ccgccgggtg agtccgcgca cgggttgcgc
      841 agtctcgaag acgggttcgt gccacccgtc ttcgatcgtg aagtgctcga ccgccggatg
      901 atggtgcaga cgcgcgactc gatcgagacg aaccggcggc tgctcgagga gtgtggggtg
      961 ttctcgggca tctcgtcggg cgcggccgtc gccggcgcga tcaagatcgc caagacgatg
     1021 gataccggca ccatcgtcac gttgctcccc gacggcggct ggaagtacct ctcgtcgggc
     1081 gcgtggaccg atccggtcga gaccgtcatc gagcgcgcca ccaagatcaa ctactggtga
     1141 gctcgatctc ggacgcagtc gccgcgctgc ggcgcggcgg gctggtcgcg ttcccgaccg
     1201 aaaccgtgta cggcctcggt gccgacgccg cgaaccgcga cgccgtccgg cggctctacg
     1261 cggtgaaggg ccgtccaccg gatcacccgg tcatcgcgca catcggccgc gacgcgacgc
     1321 tcgacgagta cgcgcgcgac gtgccggccg ttgccaacgt gctcgcgcgc gcgttctggc
     1381 ccggtccgtt gacgttgatc ctgcatcggc ggccggaacg tgtcgtcgcg gaagtcgcgg
     1441 gcggtcgcgg cacggtggga ttgcgcgtgc ccgaccacga catcgcgctc gacctgctcg
     1501 acgcgttcgg cggtgcgatc gcggcgccgt ccgcgaaccg tttcgggcgc gtcagcccga
     1561 cgacggccgc gcacgtacgc gccgatctcg gcgaagacgt cgacgtcgtg ctcgacggcg
     1621 gcgcgacgcg cgtcggcgtc gagtcgacga tcgtcgattg cacgaccggc ggcctgaccg
     1681 tcttgcgcgt cggtgccatc agcgcggcgc agctcgcgga ggtcaccggt ggcgacgtac
     1741 ccttcggcac cggtgtcgcc gcgccgggca ctctcgcctc gcactatgcg ccgcgggcgc
     1801 gggtggagtt ggtcgatcgg ggtgatgtcg acgcgcggct ggctgcggtg cgggccgagg
     1861 gcctgcgggc cggggtgctc gacgcgccgg ccgacgtcgc ggagtacgcg cgcgtgctct
     1921 acgcgcgcct gcgggcggcc gacgacgacg gcctcgaggt cgtgctcgcg gtgttgccgc
     1981 ccgacgacgg cggtctgggg acggtcgtcc gcgatcgcct ccgccgcgcc gccggctgac
     2041 tttcgcgaaa tcgacccatt cggacgcctg tgtgcccagc gtcacaccgg tttcacggcc
     2101 tgcggcgggt agcgtttgtc ccgaatggcc tccgaccccc gaccgatcgg cgtgttcgac
     2161 agcgggctcg gtgggctgac cgtcgcccgg gcgctcctcg atctcgtgcc cgggatcccc
     2221 ctcgtctact tcggcgacac cggccgattc ccgtacggac ccaagccggg agccgttgta
     2281 cgggtgtatg cgtgcgagat cgcggacctg ctcatggcgc ggggcgtgca gatgatcgtc
     2341 gtggcctgca acagcgcgac ggccgccgcc cttgaggacc tccgcgcgcg cgtcgatgta
     2401 ccggtcgtgg gcgtcatcga gccgggcgtg cgagccgcgc gccaggcgac gaagaacggg
     2461 cgggtcggcg tgatcggcac cgtcgggacg atctcgtcgg gcgcgtacca acgcgtcgcg
     2521 gccgacgtcg cgcccgaggt cgaagtcgtg tgcgcggcgt gtccgggatt cgtcgagttc
     2581 gtcgaggcgg gtgacgtcga ctccgaccag gtgcacgtgc ttgccgaacg gctacttgcg
     2641 cccgtcgtcg cggccaaggt cgacacgctc gtgctcggct gcacgcacta tcccttgctc
     2701 gcgcgcacga tcagcgacgt catgggtccc gacgtcgtgc tcgtgtcgag cgcggacgag
     2761 accgcgttcg aagtgcggcg cgaactgaat gcaacgaccg gcgcagacgc gatgtcgcgc
     2821 gcggcaacac cgatgttcat gacgagcggc gaagtcgaca cgttccgcac gctcggtgca
     2881 cgattcctcg gtcccgaagt cgagcacgtg gaggcgtggt cgtggagctg acagtgctgg
     2941 ggtcctccgg ctcgtacggt gcgcccgcgg gcggcgcgtg cagcggttat ctcctccggg
     3001 cgggcgatgt tgccatctgg atggattgcg gcaacggcac gctcgtcaat ctgcagcaac
     3061 acgtgaaccc cgccgagctc accgccgtgg tcatcacgca cggccatccc gaccactgcg
     3121 tcgacatcta cggcttgcac gtgatgaaca agtacggcat cgaccgcgcg atgttgccgg
     3181 tcttcgcggc cgaaggcgtg gagaagcaac tcgagcatct cgtgggcaac tggaccgaca
     3241 cgttcgactg gcacacggtc ggcgacggtg accgcgccca actcgacggg gtcggtctgc
     3301 gcttctcgcg caccgaccac ccgccgccga cgacggcggt cgagatcagc cacgagggca
     3361 agcgcctcgt ctacacctcc gacaccggtc cggaatggag ctgcgaggtg ttcggcgcgg
     3421 gcgctgactt cgtactgtcc gaggcgacgt accagcacga cgacatccgc gcgcccattc
     3481 acctctcggc gcaccaggcc ggcaccgcgg cgcgcgacgc gcacgccaag cagctgatgc
     3541 tcacccacat ctggccgacg ctcgacccgg tagcctccgt cgcggaagca tccgaggcgt
     3601 acggcggggt cgtggaactt gcggcaccgc accgcaagat ccacatttga gcgaatccag
     3661 ctgaacgaaa gcgacatggc gacacgagca tccggccgcg accccgacga gctgcgcggg
     3721 gtgacgttca cccgcgacta cacgagcatg gcgatgggct ccgtgctggt cgagttcgga
     3781 cgcacgcgag tgctctgcac cgcatccgtc gaagaacgga ttccgccctg gctgcgcaac
     3841 tccggcaagg ggtgggtgac ggccgagtac tcgatgctcc cgggctcgtc ggccgagcgc
     3901 gtcgaacgcg aagccgccaa gggcaagcag tcgggccgca cacaggagat ccaacggttg
     3961 atcggtcgtt cgttgcgcgc ggtcaccgat ctgcgcgcga tgccggaggt gcagatcacc
     4021 gtcgattgcg atgttctgca ggccgacggc g
//
LOCUS       DASUGA010000546         2173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1414522, whole genome shotgun sequence.
ACCESSION   DASUGA010000546 DASUGA010000000
VERSION     DASUGA010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414522"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="VFX21_10860"
     CDS             <1..960
                     /locus_tag="VFX21_10860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006538566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX5096508.1"
                     /translation="AAVCSEYGGPDVVRVSDAPSPALGSGQVRVRVRAAAVNFPDVLL
                     VANKYQISVPTPFVPGSEFAGEVVEGDGLPAGTRVFGSCLNGAFAEEVVAPAASLART
                     PDNVDDAHASAFGVAHRTAYHVLRSFAGVQPADEVVVLGAGGGVGLATVQLATALGAS
                     VTAVASSDEKLAVAAAYGARNVVDHRAGPLRDALRQVLPHGADVVVDPVGGELSEPAL
                     RALHYGGRFVTVGYASGEIPRIPLNLVLLKGVVVRGFQMIDFLAHEPDELARNEAELL
                     ILLAEGRATPHIGATFAFADVAAALQYVADGRAIGKVVLDVSG"
     gene            947..>2173
                     /locus_tag="VFX21_10865"
     CDS             947..>2173
                     /locus_tag="VFX21_10865"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5096509.1"
                     /translation="MSVAEDSLRVVIVGAGFGGIAMGVKCKRAGIDTFTIYEASLGIG
                     GTWWDNTYPGAEVDVGSQLYCFSFKPYAWTRTHARQPELQKYLEETVDEFGLRPHLRL
                     GTTVQSATWDDDAHTWSVLLDDGTVDVCNVLVSAVGFLNVPRLPAWPGLDEFEGPVFH
                     SARWEHQHDLTGKTVAVVGTGSSATQVVPALQPIAGRLHVYQREPGWILAKGERDLDD
                     EERSQFARPWNRYRERLRLRYLLEKSLWRGDLVRPGTKVNAAREEMCRKYIAKQFKDH
                     PDLREAVTPKYPYPGKRPIFASTFYPALKKENVELVPKAVSALTRTGIVDTDGVERAA
                     DVVVLATGFQPTNYLARLKVVGRGGRSLRELWGDEPRAYLGITVPGFPNFYMLYGPGT
                     NGGEIVTMLEAQARYAL"
BASE COUNT          325 a          723 c          782 g          343 t
ORIGIN      
        1 gccgccgtat gttccgagta cggcggtcct gatgtcgtgc gcgtttccga cgcaccgtca
       61 ccggcgcttg gctcgggaca ggtgcgcgtt cgggtgcgcg ccgcggccgt caactttccc
      121 gacgtgctgc tggtcgcgaa caagtaccag atcagcgtgc cgacgccctt cgtgccgggg
      181 agcgagttcg cgggggaggt cgtcgagggc gacggcttgc cggccggcac ccgggtgttc
      241 gggtcgtgcc tgaacggtgc gttcgcggag gaggtcgtcg cgcccgccgc atcgctcgcg
      301 cgcacgcccg acaacgttga cgacgcgcac gcgtcggcgt tcggcgtcgc ccaccgcacg
      361 gcgtaccacg tgctgcgttc cttcgcgggc gtgcagccgg ctgacgaggt cgtcgtgctc
      421 ggagcgggcg gcggcgtcgg cttggcaacc gtgcagctcg cgacagcact gggcgcgtcc
      481 gtgacggcgg tcgcgtcgag tgacgagaag ctcgcggtcg cggccgcgta cggtgcgcgg
      541 aacgtcgtcg accatcgcgc cggacccttg cgcgatgcgc tgcgacaagt gctgccgcac
      601 ggcgcggatg tcgtcgtcga tccggtcggt ggcgaactgt cggagccggc actgcgcgca
      661 ctgcactacg gcggccggtt cgtcaccgtc ggctacgcgt cgggcgagat cccgcgcatc
      721 ccgctcaatc tcgtcctgct caagggcgtc gtcgtgcgcg ggttccagat gatcgacttc
      781 ctcgcccacg agcccgacga gctggctcgg aacgaagcgg agctgctcat actgctcgcc
      841 gagggccgcg cgacaccgca catcggcgcg acgttcgcgt tcgccgacgt ggcggccgcg
      901 ctgcagtacg tcgcagatgg ccgggccatc ggcaaggtcg tcctcgatgt cagtggctga
      961 ggactcgctg cgcgtcgtca tcgtgggtgc ggggttcggc ggcatcgcga tgggggtgaa
     1021 gtgcaaacga gccggcatcg acacgttcac gatctacgag gcatcgctcg gcatcggcgg
     1081 cacgtggtgg gacaacacgt accccggcgc ggaggtcgac gtcggctcgc agctctactg
     1141 cttctcgttc aagccgtacg cgtggacgcg cacgcacgcg cggcagccgg agctgcagaa
     1201 gtacctggag gagacggtcg acgagttcgg gctgcggccg cacctgcggc tcggcacgac
     1261 ggtgcagtcg gctacgtggg acgacgacgc gcacacgtgg agcgtgctcc tcgacgacgg
     1321 caccgtcgac gtgtgcaacg tgctggtcag cgccgtcggc ttcctcaacg tgccgcggct
     1381 cccggcgtgg ccgggtctcg acgagttcga aggaccggtg ttccacagcg cgcgctggga
     1441 gcatcagcac gacctcaccg gcaagacggt cgccgttgtc ggcacgggat cgtcggcgac
     1501 gcaggtcgtc cccgcgctgc aaccgatcgc cggccggctg cacgtgtacc aacgcgaacc
     1561 gggctggatt ctcgccaagg gcgagcgcga cctcgacgac gaggaacgtt cgcagttcgc
     1621 gcggccgtgg aaccggtacc gggaacgctt gcgcctgcgg tacctgctcg agaagagcct
     1681 ctggcgcggc gatctggtgc gacccggcac gaaggtcaac gccgcgcgcg aagagatgtg
     1741 ccggaagtac atcgcgaagc agttcaagga ccaccctgac ctgcgcgagg cggtgacgcc
     1801 gaagtacccg tatcccggaa agcggccgat cttcgcgtcg acgttctatc ccgcgctgaa
     1861 gaaggagaac gtcgagctgg tacccaaggc ggtgtcggcg ttgacccgta ccggcatcgt
     1921 cgacaccgac ggcgtcgaac gcgcggccga tgtcgtcgtg ctcgcgaccg gcttccagcc
     1981 caccaactat ctggcgcgcc tcaaggttgt cggtcgcggc ggacgttcat tacgcgagct
     2041 ctggggcgac gagccgcgcg cgtacctcgg tatcacggtg ccgggcttcc cgaacttcta
     2101 catgctctac ggaccgggga cgaacggcgg cgagatcgtg acgatgctcg aagcgcaggc
     2161 gcgctacgcg ctg
//
LOCUS       DASUGA010000547         3631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_95493, whole genome shotgun sequence.
ACCESSION   DASUGA010000547 DASUGA010000000
VERSION     DASUGA010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95493"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VFX21_10870"
     CDS             complement(<1..747)
                     /locus_tag="VFX21_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096510.1"
                     /translation="MVARTTSFARRGRALTIASGMLVVLGLATALPALGAPAAFPGPA
                     NLHRDIDDYVLFAYTQLNFKGGNGQVAQIDGDVGVNEADPDQNDGNAVLNICQNGKIE
                     FVNDAQLVGDSAKLDQCTLGDLFVNTQLGSGTPTINGSISDWGTPIIQPQDLPVFPSF
                     QCNNDHKTVAKNGSLDLLPGAYGNLDMKDGATLTLHAGTYTFCDFKAGKKAAVETEDA
                     TVVQIAGELKINNGDDGDDADAGDTEFGSAC"
     gene            complement(856..2262)
                     /locus_tag="VFX21_10875"
     CDS             complement(856..2262)
                     /locus_tag="VFX21_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096511.1"
                     /translation="MGTARRRGRVVSVAAIALIAGGLSQMLPASASGAPSRPVDRDID
                     TYVLFGFESVNFKGAESAGRGIISGGNVGANGIDTDPADSEVVVNICANHNVTMDHRS
                     QLVGDSVRATDGCHVWDVYANSLIGSPAVVPANSGPTAFATPVVTAPAFPSFACNAAA
                     PVDVGTGATSMLPPGTYGAVKFKDGSTVTLGAGTYTMCDLNIGKNVNVTTVTGTVLQI
                     EQTFSVSNETVFGPVCDVPIYVRADGATGDNDVAVNFSKHTSVAGKFFTLLGKIALGN
                     DTDLEGNFWGRRLISDADVNVEGCRPPPATTTTTSGGGATSSSSSSSTATSSSATSTS
                     TTSTSTTTTTTTVPASTTSTTATVVTTTSTTAGASSTTTTQPATTTSLLGTTTSSTAA
                     TTTSGPELTVGGVTSSVPLAPGAASSEPTPSLTQGELPRTGGGSEGFPVGIALIVAGL
                     ALLGVGQRPQLRRWWPGA"
     gene            complement(2405..2812)
                     /locus_tag="VFX21_10880"
     CDS             complement(2405..2812)
                     /locus_tag="VFX21_10880"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX5096512.1"
                     /translation="MVDPFVHRIRTRYAEIDGQGVVFNAHWLTYFDDTCTRFMQSLDL
                     GARFWLGEFDVMLVHAELEWQGPAYFDEWIDIAVAPVRLGRSSFDLRYRAAVGERPAC
                     TATITYVGIVPGKNEPVELPSGLRVAFSARMPK"
     gene            complement(2818..>3631)
                     /locus_tag="VFX21_10885"
     CDS             complement(2818..>3631)
                     /locus_tag="VFX21_10885"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX5096513.1"
                     /translation="ESRVIEVENPLRAEESILRPRLLPGLLRAVAFNASHGNPDVALF
                     ELGTVFAPPNRAHDTLPEERLHLAAVRSGRVRRMPHEPDRDVIAADLVAALDAVAAEL
                     RLADVRLEATDWPGFHPVRTARVCVDGNTVGVVGEVDADVVAALELPAPVDAFEVEVD
                     ALLAGARRPRTARPVSRFPASTIDLAFVVDDTEPAGEILRTLEAAAGELCERVELFDV
                     FRSDALGVGKVSLAFSVRFRALDRTLTDDEVASLRRQCIDAVTKTHAATLRA"
BASE COUNT          545 a         1211 c         1288 g          587 t
ORIGIN      
        1 gcacgcggag ccgaactcgg tgtcgccggc gtcggcgtcg tcaccgtcgt cgccgttgtt
       61 gatcttcagc tcaccggcga tctgcacgac ggtcgcgtcc tcggtctcga ccgcagcctt
      121 cttgcccgcc ttgaagtcgc agaaggtgta ggtgccggcg tgcagggtca gcgtcgcgcc
      181 gtccttcatg tcgaggttgc cgtacgcgcc cggcagcagg tcgagcgaac cgttcttcgc
      241 gaccgtcttg tggtcgttgt tgcactggaa gctcgggaac accggcaggt cctgcggttg
      301 gatgatcggc gtgccccagt cggagatgct gccgttgatc gtcggtgtgc cgctgccgag
      361 ctgcgtgttc acgaacaggt cgccgagcgt gcactggtcg agcttggccg agtcaccgac
      421 gagctgggcg tcgttgacga actcgatctt gccgttctgg cagatgttga ggaccgcgtt
      481 cccgtcgttc tggtccgggt cggcctcgtt gacgccgacg tcgccgtcga tctgcgccac
      541 ctgcccgttg ccgcccttga agttcagctg tgtgtacgcg aacaagacgt agtcgtcgat
      601 gtcgcggtgg aggttggccg ggccggggaa ggccgctggc gcaccgagcg ccggaagtgc
      661 ggtcgccaga ccgagcacga cgagcatgcc gctggcaatc gtcagcgcgc ggccgcgccg
      721 cgcgaacgaa gtggtcctgg caaccatggc tgtagctctc ccgtcccgtc ttcgcacgcc
      781 ctgcgaagtg cggttactca accacggaga gtgagctttc gcaacgtgac aaaggccatg
      841 gtcccgcccg tggcctcaag cgccgggcca ccaccgccga agctgcggcc tttgcccgac
      901 gcccagcagg gccagaccgg cgacgatcag tgcgatcccg accgggaagc cctcgctccc
      961 acctccggta cgcgggagct cgccttgggt gagactcggc gtcggttccg acgatgccgc
     1021 accgggtgcg agcgggacac tggacgtcac accgccgacc gtgagctcgg gccccgacgt
     1081 cgtcgtggcc gcggtcgacg aggtcgtcgt gccgagcagc gacgtggtcg tcgcgggctg
     1141 cgtcgtggtc gtgctcgatg cacccgcggt cgtcgaagtc gtggtgacca ccgtcgcggt
     1201 cgtcgaggtc gtcgacgccg ggaccgtcgt cgtcgtcgtg gtcgtgctcg tcgaggtggt
     1261 cgacgtcgac gtcgccgagg aagaagtggc cgtcgacgag ctgctggagg acgaggtcgc
     1321 gccaccgccg ctcgtcgtcg tggtcgtggc gggcggcggc cggcagcctt cgacgttgac
     1381 gtcggcgtcg ctgatgagtc gccggcccca gaagttgccc tcgaggtcgg tgtcgttgcc
     1441 gagcgcgatc ttcccgagca acgtgaagaa cttgccggcg accgacgtgt gcttcgagaa
     1501 gttgaccgcg acgtcgttgt cgccggtggc gccgtcggcg cgcacgtaga tcggcacgtc
     1561 gcagactgga ccgaacaccg tctcgttgct gaccgagaaa gtctgctcga tctggagcac
     1621 cgtgcccgtg accgtcgtga cgttcacgtt cttgccgatg ttcaggtcgc acatggtgta
     1681 ggtgccggcg ccgagggtca cggtcgaacc gtccttgaac ttcaccgcgc cgtacgtgcc
     1741 gggcggcagc atcgacgtcg ccccggtgcc gacgtcgacc ggcgcggccg cgttgcacgc
     1801 gaaggacggg aacgccggtg ccgtgacgac gggcgtcgcg aacgcggtcg gccccgagtt
     1861 ggcgggaacg accgccgggc tgccaatgag tgagttcgcg tagacgtccc agacgtggca
     1921 accgtcggtg gcccgcacgg aatcgccgac gagctgtgag cggtggtcca tcgtcacgtt
     1981 gtggttcgca cagatgttca cgacgacttc gctgtcggcg gggtcggtgt cgatgccgtt
     2041 ggcgccgacg ttgccgccac tgatgatgcc gcgccccgcg gactccgcgc ccttgaagtt
     2101 gacgctctcg aagccgaaga gcacatacgt atcgatgtcg cggtcgaccg gtcgcgacgg
     2161 cgcgccgctc gcggacgccg gcaacatctg tgacaggccc ccggcgatca acgcgatcgc
     2221 cgcgaccgaa acgactcgtc cgcgcctgcg ggccgttccc atgcacccca ccgtgggtcg
     2281 gaccatccgt ggtggtgacg ctccgtgcct tcgcttgccg gtgcgggcgc gcgcagaccc
     2341 ctgccgctcg actccgccga gggtgacaat aaaccaatcg cggtgctatt tccgcgtcaa
     2401 cgccctactt cggcattcgc gccgaaaatg caacacggag tcccgaaggc agctcgaccg
     2461 gttcgttctt cccgggcacg atgccgacat aggtgatcgt ggcggtgcac gccgggcgct
     2521 cgcccacggc ggcgcgatag cgcaggtcga acgacgagcg gcccaggcgg acgggcgcga
     2581 cggcgatgtc gatccactcg tcgaaatacg ccggcccctg ccactcgagc tccgcgtgca
     2641 cgagcatcac gtcgaactcg ccgagccaga accgcgcacc gagatccagc gactgcatga
     2701 agcgcgtgca ggtgtcgtcg aagtaggtga gccagtgggc gttgaagacg acgccctgcc
     2761 cgtcgatctc cgcgtaccgc gtgcggatgc ggtgcacgaa gggatcgacc atgccggtca
     2821 cgcgcgcaga gtcgcggcgt gcgtcttggt gacggcgtcg atgcactgcc ggcgcagtga
     2881 cgcaacctcg tcgtcggtga gcgtgcggtc gagtgcacgg aaccgcaccg agaacgcgag
     2941 gctcaccttg ccgacgccga gcgcgtcgga tcggaagacg tcgaagagct cgacgcgctc
     3001 gcacagctca cccgcggcgg cctcgagcgt acgcaggatc tcgcccgcgg gttcggtgtc
     3061 gtcgacgacg aacgcaagat cgatcgtcga cgccggaaag cgcgagacgg gacgggccgt
     3121 gcgcggccgc cgggctcccg ccagcagcgc gtcgacctcg acttcgaacg cgtcgaccgg
     3181 cgcgggcagc tcgagcgcgg cgacgacatc ggcatcgact tcgccgacga cgccgaccgt
     3241 gttgccgtcg acgcacacgc gcgcggtgcg caccgggtgg aagccgggcc agtcggtcgc
     3301 ctcgaggcgc acgtcggcga ggcggagctc ggccgcgacc gcgtcgaggg cggcgacgag
     3361 atcggcggcg atcacgtcgc gatccggttc gtggggcata cgccgcacgc gccccgatcg
     3421 cacggccgcc agatgcaggc gttcctcggg cagcgtgtca tgcgcgcggt tcggcggcgc
     3481 gaatacggtg ccgagctcga aaagcgcgac atccgggttg ccgtgcgacg cgttgaatgc
     3541 gactgcgcgc aacaggccgg gcaggagccg cgggcgcagg atcgattctt cggcccggag
     3601 cgggttctcg acttcgatga cgcggctctc g
//
LOCUS       DASUGA010000548         3976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1029293, whole genome shotgun sequence.
ACCESSION   DASUGA010000548 DASUGA010000000
VERSION     DASUGA010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029293"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VFX21_10890"
     CDS             <1..751
                     /locus_tag="VFX21_10890"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX5096514.1"
                     /translation="RDGYALLEGVFDAEMVARVRAEIDDVFERIPPERFREDKAEFRY
                     ELYNRSAACQEAIGHPRILEAIEPLLGEDCHVIANTAWRNPSNFEGGVWHCDAGPHVP
                     RPEGVPWPDDIPYPFFAVGAHLYLQDCALVDGPTAVVPGSHRSGRLAPFDKLLDDDLT
                     YDGRPPVVIEATAGDVALFVSDAWHRGLPAQEGGRGRYFLQAHYARRDIAQRVRITSE
                     ANQCSDEAIARATTPREQELIGMHGSFFYDA"
     gene            764..1852
                     /locus_tag="VFX21_10895"
     CDS             764..1852
                     /locus_tag="VFX21_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX5096515.1"
                     /translation="MTTDDERIALFLDYENLAIGARDLMAGGVLQFRPIADALAERGR
                     VVVRRAYADWSRFDEDRRELTRHHVELIEIPQRMGLVRKNAADIKMAVDAVELSFERD
                     YITTFVLGTGDSDFTPLVHKLRELNKRVIGIGIEASTSALLPPACDEFLFYERLDGVE
                     LATPKKKKTTRSTTKSTSKRVPEPADTGDEPALVQPSDTDLDRLVTQTLAGLERGTSG
                     PVVASTLKRTILRKDPTFNETTYGFRGFLELLRHLESLGVIALSEGTNQGDPAITLAG
                     ETEDEDKAFEALGRIVAKATAKSGPQPLSGLKTQLRRSIPGFSEKRFGYGGFLQFAKA
                     ARARGVIELDFDDEVGDYVVRPGSAATP"
     gene            complement(1782..3014)
                     /locus_tag="VFX21_10900"
     CDS             complement(1782..3014)
                     /locus_tag="VFX21_10900"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5096516.1"
                     /translation="MHSFRELGVPADLAAVLERRGITAPFEIQELTIPDLIAGRDICG
                     RAPTGSGKTLAFGIPLVARVGHAGPKRPRALVLAPTRELAAQIERELKPLAAARDRRV
                     LTVYGGVGYEHQRKALRKGVDILVACPGRLADLIEQRVVRLDGIDTVVIDEADRMADM
                     GFLPQVRALLDEMPPNRQTVLFSATLDGAVGVLTREYQRNPMRHEFGEIEPDITAADH
                     HFWTVEPPERVRRTAEVVNAAGSTIVFCRTRRGVDRVVEVLGRDGVKAAAIHGGRSQN
                     QRDQALRSFTNGRVDVLVATDVAARGIHVDDVACVVHFDLPADAKDYVHRSGRTARAG
                     ARGTVISLVAKNDARKARRLQREAGIDVPLQKPQIERLETRPRPARKVVERDRAKASP
                     PNPAARRSRRPHRRNRVR"
     gene            complement(3068..3520)
                     /locus_tag="VFX21_10905"
     CDS             complement(3068..3520)
                     /locus_tag="VFX21_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096517.1"
                     /translation="MSARLSLNHVAITMDPSLLDDAGRQDLLAFYGDVFGWTEGDNTG
                     EPGNPLIMYTGAFGEFVYLLPGDEPLTAPALDHFGYQVDSLADLADMVGRVKAWKARD
                     GRVQLIDVHSRTTHGPAHDYTLTSAYVGFVLPLMIELQHLERHAERLS"
     gene            complement(3517..>3976)
                     /locus_tag="VFX21_10910"
     CDS             complement(3517..>3976)
                     /locus_tag="VFX21_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5096518.1"
                     /translation="TDITVSEPSPLRRARALAVGAAQAIEPFALARAPMGRTVESPFT
                     IAFECSGNNYAVESAFDQLDYAGTLVFVGTGHDMPTINHNRAIVLELTIIGAYNYDAD
                     GFESAVAMLASGVLPVDLLIDPVDAPLAGVLDAMLECARGERPAKVMVKP"
BASE COUNT          640 a         1337 c         1392 g          607 t
ORIGIN      
        1 ccgtgacggc tacgcgctgc tcgagggcgt gttcgacgcc gagatggtcg ctcgcgtgcg
       61 ggccgagatc gacgacgtgt tcgaacgcat cccgcccgag cgtttccgtg aggacaaggc
      121 cgagttccgc tacgagctgt acaaccgcag cgccgcgtgc caggaagcga tcgggcatcc
      181 gcgcatcctc gaagcgatcg agccgctgtt gggcgaggac tgccacgtca tcgcgaacac
      241 cgcgtggcgc aacccgagca acttcgaagg cggcgtgtgg cactgtgacg cgggcccgca
      301 cgtgccgcgg cccgagggcg tgccctggcc cgacgacatc ccgtacccgt tcttcgcggt
      361 cggcgcgcac ctctacctcc aggattgcgc gctcgtcgac ggccccaccg cagtcgtccc
      421 cggcagccat cgctcggggc ggctggcacc gttcgacaag ttgctcgacg acgacctcac
      481 ctacgacgga cgaccgccgg tcgtgatcga ggccacggcg ggcgacgtcg cgctgttcgt
      541 ctccgacgcg tggcaccgcg gcctgccggc gcaggagggt ggccgcggcc ggtacttctt
      601 gcaggcgcac tacgcccgac gcgacatcgc gcagcgggtg cgcatcacct ccgaagcgaa
      661 tcagtgctcc gacgaggcga tcgcgcgcgc aacgacgccg cgcgagcaag agctgatcgg
      721 catgcacggt tccttcttct acgacgcatg acgggctcca catatgacta ctgacgacga
      781 gcgaatcgcg ctcttcctcg actacgagaa cctcgcgatc ggtgcgcgcg atctcatggc
      841 cggcggcgtg ctccagttcc ggccgatcgc ggacgcgctg gccgaacggg gacgtgtcgt
      901 cgtgcgccgc gcctacgccg actggagccg tttcgacgaa gaccgccggg agttgacgcg
      961 ccaccacgtc gagctgatcg agatcccaca gcgcatgggc ctcgtgcgca agaacgcggc
     1021 cgacatcaag atggcggtcg acgcggtgga gctctcgttc gagcgcgact acatcacgac
     1081 gttcgtgctc ggcaccggcg acagcgactt cacacccctt gtgcacaagc tgcgcgagct
     1141 caacaagcgc gtgatcggta tcggcatcga ggcgtcgacg tctgcgctct tgccgccggc
     1201 gtgtgacgag ttcttgttct acgaacggct cgacggcgtc gaactggcta cgcccaagaa
     1261 gaagaagacg acgcgcagca cgacgaagtc gacctcgaag cgcgtcccgg aaccggcgga
     1321 caccggtgac gaaccggcgc tcgtgcaacc atccgacacc gacctcgacc ggctcgtcac
     1381 ccagacgctc gccggcctcg aacgcggcac gagtgggccg gttgtcgcgt cgacgctgaa
     1441 gcgcacgatc ctgcgcaagg atccgacgtt caacgagacg acgtacgggt tccgtggctt
     1501 cctcgaactg ttgcggcacc tcgagtcgct cggcgtcatc gcgctgagcg agggcaccaa
     1561 ccagggcgat ccggcgatca cgctcgcggg cgagaccgaa gacgaggaca aggcgttcga
     1621 ggcgctcggt cgcatcgtcg ccaaagcgac cgcgaagtcg ggcccgcaac cgctctccgg
     1681 actcaagacg caactacgca ggtcgatccc gggcttcagc gagaagcgtt tcggctatgg
     1741 cggattcctg cagttcgcca aggccgcccg ggcgcgcggc gttatcgaac tcgatttcga
     1801 cgatgaggtc ggcgactacg tcgtgcggcc gggttcggcg gcgacgcctt agctcggtcg
     1861 cgttcgacga ccttgcgggc cgggcgcggc cgcgtctcga gccgttcgat ctgcggcttc
     1921 tgcagcggca cgtcgatgcc ggcttcgcgc tgcagccgcc gcgccttgcg ggcatcgttc
     1981 ttggcgacga gcgagatgac ggtgccgcgg gcgcccgctc gcgcggtgcg acccgaccgg
     2041 tgcacgtagt ccttggcgtc ggcgggcagg tcgaagtgca cgacgcatgc gacgtcgtcg
     2101 acatggatgc cgcgtgccgc gacgtcggtc gcgacgagca cgtcgacgcg gccgttggtg
     2161 aaggagcgaa gcgcctgatc acgctggttc tgcgaacgac caccgtggat ggcggcggcc
     2221 ttcacgccgt cgcggccgag cacttcgacg acgcgatcca caccgcgccg cgtacgacag
     2281 aacacgatgg tcgaccccgc ggcgttgacg acctccgcgg tgcgccgcac gcgctcgggc
     2341 ggttctacgg tccagaagtg atggtcggcg gcagtgatgt cgggctcgat ctcaccgaac
     2401 tcgtggcgca tcgggttgcg ctggtactcg cgcgtgagca caccgaccgc gccgtcgagc
     2461 gtcgcggaga acagcaccgt ctggcggttc ggcggcatct cgtcgagcag cgcgcgcacc
     2521 tgcggcagga agcccatgtc ggccatgcgg tcggcctcgt cgatcacgac ggtgtcgatc
     2581 ccgtcgaggc gaaccacgcg ctgctcgatg aggtcggcga ggcggcccgg gcacgcgacg
     2641 agaatgtcga cacccttgcg caacgccttg cgctggtgct cgtaaccgac accgccgtac
     2701 acggtgagga cgcgccgatc acgcgccgcc gcgagcggct tcagctcgcg ttcgatctgc
     2761 gccgcgagct cacgggtcgg cgcgagcacg agcgcacggg gacgcttggg tccggcgtgg
     2821 ccgacgcgcg cgaccagcgg gatgccgaac gcgagcgtct tgccggagcc ggtcggtgcg
     2881 cggccgcaga tgtcgcgccc ggcgatcagg tcggggatgg tgagctcttg gatctcgaac
     2941 ggggcggtga tgccgcgccg ttcgagcacg gcggcgaggt cggcggggac gccgagttcg
     3001 cggaaggaat gcacggaaac tcctggtcag cttgcgttga ccactcggag tcatcgcacg
     3061 gaatgaatca ggatagccgc tccgcgtgcc gttccaggtg ctggagctcg atcatcagcg
     3121 gcagcacgaa gccgacgtac gcactcgtga gcgtgtagtc gtgggccggc ccgtgtgttg
     3181 tgcgggagtg cacgtcgatc agctgcaccc ggccgtcgcg cgctttccat gccttcacgc
     3241 ggccgaccat gtccgcgaga tcggcaagcg agtcgacctg gtagccgaag tggtcgagcg
     3301 cgggtgcggt caacggctcg tcgccgggca ggaggtagac gaactcgccg aacgcgcccg
     3361 tgtacatgat caacgggttg ccgggttcgc cggtgttgtc gccctcggtc cagccgaaga
     3421 cgtcgccgta gaacgcgagc aggtcctggc ggcccgcgtc gtcgaggagc gaagggtcca
     3481 tcgtgatcgc gacgtggttc aggctcagtc gcgcgctcac ggcttcacca tcactttggc
     3541 cggacgttcg ccgcgtgcgc actccagcat cgcgtcgagc actcccgcca gcggcgcgtc
     3601 gaccgggtcg atcagcaagt cgaccggcaa cacgcccgac gccaacatcg ccacggcgga
     3661 ctcgaagccg tcggcgtcgt agttgtacgc gccgatgatg gtcagctcga gcacgatcgc
     3721 gcggttgtgg ttgatcgtcg gcatgtcgtg accggtgccg acgaacacga gcgtgcccgc
     3781 gtagtcgagc tggtcgaacg ccgactcgac ggcgtagttg ttccccgagc actcgaacgc
     3841 gatcgtgaac ggcgactcga cggtgcggcc catcggggcg cgcgcgagcg cgaacggctc
     3901 gattgcctgc gcggcaccga cggcgagcgc gcgtgcgcgg cgcagcggcg acggctcgga
     3961 gacggtgatg tcggtg
//
LOCUS       DASUGA010000549         2057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_716193, whole genome shotgun sequence.
ACCESSION   DASUGA010000549 DASUGA010000000
VERSION     DASUGA010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716193"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /gene="manB"
                     /locus_tag="VFX21_10915"
     CDS             complement(<1..582)
                     /gene="manB"
                     /locus_tag="VFX21_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014142783.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX5096519.1"
                     /translation="MVLDAIFKAYDVRGVYPDELDEALARRIGNAFANFTGATAMLVG
                     RDMRPSSEPLVAAFIEGATLAGADVTDLGLCSTDLVYFAAGALDAPGAMFTASHNPAQ
                     YNGIKFCKAGAAPVGEQSGLAQIKELVASGVTSRGDSPGKVEQRDLLDEFRAHVQSFV
                     DTSVLRPLKVVADTANGMGGLVVPKVFEGLPFEL"
     gene            complement(641..>2057)
                     /locus_tag="VFX21_10920"
     CDS             complement(641..>2057)
                     /locus_tag="VFX21_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096520.1"
                     /translation="LLHAEALDVVAEGRWDRDPPDWEAPSRLDQDHTKRRAAFVSALA
                     LAIVVALVGAFALNRAWDASDNNKGIDIAGWARWNYHGYEDKAAWPEYQSIMQVMGSL
                     PCGRALWEPSSMEGDPINNYGTSLALELLPYYTKGCIGSMEGLYFESSATTSYHFLTV
                     SELAQHPSNPVRGLVWGTLANDFDTGVKHLQMLGVKYYMAWTPEARAKADRNADLRLV
                     ATVPDKDGADPRGWYIYEVQDAPLVEGLASEPVVAKLHSGSKSECFGTPPEQDKKQDP
                     HLDAWECSAASWWRTASLLSQQWVESGPKEWQRVDFDDLGNVDEKKLPAVKVTDIDER
                     PDKISFHVDKVGVPVVVKTSFFPNWEASGADGPYRLAPNLMVVVPTKNDVTLHYGMTG
                     VDWLGRLFTLFGIVGLIALARWKGARRFAADPDPERGDGTAAADPEGAAAAWTPVGAG
                     PATGGDEPHPPDRSEPEPAIP"
BASE COUNT          314 a          724 c          687 g          332 t
ORIGIN      
        1 gagctcgaac gggaggccct cgaacacctt gggcaccacg aggccgccca tgccgttggc
       61 cgtgtcggcc acgaccttga gcggccgcag caccgacgtg tcgacgaacg actgcacgtg
      121 ggcccggaac tcgtcgagga gatcgcgctg ctcgaccttg ccgggcgagt cgccgcgcga
      181 cgtcacgccc gatgccacga gctccttgat ctgcgcgagc ccgctctgct ccccgaccgg
      241 tgccgcgccc gccttgcaga acttgatgcc gttgtactgc gcggggttgt ggctcgcggt
      301 gaacatcgcg ccgggggcgt ccagcgcacc ggccgcgaaa tagacgagat cggtcgagca
      361 gagcccgagg tcggtgacgt cggcacccgc cagcgtcgcc ccctcgatga aggcggccac
      421 cagcggctcc gacgacggcc gcatgtcgcg acccacgagc atcgccgtcg cgccggtgaa
      481 gttggcgaac gcgttgccga tgcggcgcgc cagcgcctcg tcgagctcgt cggggtacac
      541 gccccggacg tcataggcct tgaagatcgc gtcgagcacc acgaacgaac gtctttcccc
      601 tcatcagcga tgtacgcgca gttcccccgg aggggaccgg tcatggtatc gccggctcgg
      661 gttcggaccg atcgggagga tggggttcgt cgccccccgt ggcgggcccc gcacccaccg
      721 gggtccaggc cgccgcggca ccctcggggt cggccgccgc ggtgccgtcg ccccgctccg
      781 gatcggggtc ggccgcgaat cgccgcgcgc ccttccagcg ggcgagagcg atgaggccga
      841 cgatgccgaa cagcgtgaac agccggccca gccagtcgac accggtcatg ccgtagtgca
      901 gcgtgacgtc gttcttcgtc ggcaccacga ccatcaagtt gggtgcaagc cggtacgggc
      961 cgtccgcgcc cgacgcctcc cagttcggga agaacgacgt cttcacgacg accggcactc
     1021 cgaccttgtc gacgtggaac gagatcttgt cgggacgttc gtcgatgtcg gtgaccttca
     1081 ccgcgggcag cttcttctcg tcgacgttgc cgaggtcgtc gaagtcgacg cgctgccact
     1141 ccttcgggcc cgactcgacc cactgctgcg acagcagtga cgcggtgcgc caccaactcg
     1201 cggcggagca ctcccacgcg tcgagatgcg ggtcctgctt cttgtcctgt tccggcggcg
     1261 tgccgaagca ctccgacttg ctgcccgagt gcagcttcgc gaccaccggc tccgaggcga
     1321 ggccttcgac gagcggcgcg tcctgcacct cgtagatgta ccagccgcgc ggatcggcgc
     1381 cgtccttgtc gggaaccgtc gcgacgagcc ggaggtcggc gttgcgatcg gccttggcgc
     1441 gcgcttcggg cgtccatgcc atgtagtact tcacgccgag catctggaga tgcttcacgc
     1501 cggtgtcgaa gtcgttcgcg agcgttcccc agacgaggcc gcgcacgggg ttcgagggat
     1561 gttgcgcgag ctcgctcacc gtcaggaagt ggtacgacgt cgtggccgag gactcgaagt
     1621 acaagccctc catcgagccg atgcagccct tcgtgtaata gggcaacaac tccagcgcga
     1681 gcgacgtgcc gtagttgttg atcgggtcgc cctccatcga cgacggctcc cacagcgcgc
     1741 ggccgcacgg cagcgacccc atgacctgca tgatgctctg gtactcgggc cacgccgctt
     1801 tgtcctcgta gccgtgatag ttccagcgcg cccaaccggc gatgtcgatg cccttgttgt
     1861 tgtcggacgc gtcccacgcg cggttcaacg cgaacgcgcc gacgagtgcg acgacgatcg
     1921 cgagggcgag agcgctgacg aacgccgcgc ggcgcttcgt gtggtcttgg tcgagacggc
     1981 tcggcgcttc ccagtcaggc ggatcccgat cccagcgccc ttccgcgacc acgtcgagtg
     2041 cttcggcatg cagcaac
//
LOCUS       DASUGA010000550         7641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1689103, whole genome shotgun sequence.
ACCESSION   DASUGA010000550 DASUGA010000000
VERSION     DASUGA010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7641
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689103"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1539)
                     /locus_tag="VFX21_10925"
     CDS             complement(<1..1539)
                     /locus_tag="VFX21_10925"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX5096521.1"
                     /translation="MLRTRIFTASALAVAVVATSLPFVATATPAAAIGGAQWSKSFSG
                     WNRSSSPTIADVTGDGVPEIIHGAQDGWVRVLNAATGDNIPGWPQPEVIRPGYITPVD
                     GSPSVGDLDNDGAPEIVVPGGSTWRKAPGGVVVFRRDGSVKCSFETAGWSGNNDQGVY
                     STPALGDVDGDGFRDIVFGGWDLRVHALNRDCNELSGFPVNVEDSTWSSPALYDSDGD
                     GRLEIFIGSDQMAGGAIDWSGGEFRALDWRNGSVVELWKRRINDVFHSSPAIGDIDGD
                     GSMEVVTGGGNYYNRDEGRKIFAFNIHDGSDQGGFPVLTGAQTNSSPAIGDVNNDGVA
                     DVVAGSNDGYVRAINGHGQQLWASGLRFVNSTPGGPVASPIIADMNGDGVNDVGVANN
                     FAFFVLNGSNGAEMAAVNTFVSYESAGAVGDFGPLGWKLIVSGFDTPQNFTRIESYAI
                     PTPGKTPPWPMFHKNAGHTGAPLPTDVCRPPSNPAPHPSSASANGYWVLSSVGGVYSL
                     GAPFY"
     gene            1679..3259
                     /locus_tag="VFX21_10930"
     CDS             1679..3259
                     /locus_tag="VFX21_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096522.1"
                     /translation="MTAVDAAPSRAAGTERGIRLALAGLLGLLVVTVAHLVLAYMRHW
                     LNEDHTILWFAARDLGHLTFPQPSYYGAAYFSVFEAAPAEALRRAGVPLGTAVFVSTA
                     TLMTIGWVWFALVAWRRGRFAMAAAALAVPVVITTEFLVSADSPGGRGAGTFCAMTGV
                     AILLFAPARPVPIALFALLGGLGTLWDFGSALLVVPVGVYMLALNWRRWRVLAWGAIG
                     SVVPLAFALGQRAFYADHPDYNVHRAAGFMPKLSILRDTIREPGRYFAWLSPELSRTS
                     VVPIVAGAILLFFLARTRRAEVILPGVALVAIVLFSLSTPKALDGTPSVYVGYGRYFL
                     SVPAALWFLGWVLTEARPPSPRLQRRLVYGIAAFAVVTFAVRLVTFDDRTGDIRRVAA
                     DPARAGAPVFAADAVAARCDAVEHAADRAGVDYVVFRGDRVGAYACAARDYGTLETIH
                     PIYDRRTWLLHDANEENRTKFLVTDVDEGWCINARAIVESCELDPVDSTIAVVTTAPQ
                     PVLETWRQLGEEIRPFDQ"
     gene            complement(3253..3975)
                     /locus_tag="VFX21_10935"
     CDS             complement(3253..3975)
                     /locus_tag="VFX21_10935"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX5096523.1"
                     /translation="MTRFTVAGFNAHWGIDRNGSTNRRRFDVVPIVRSFDADLVVIPE
                     SFRYDDGRTILDGLEDTYEIRSVAFSRLFDETDGSHKRYRGFEPGTWELAVLSRLPAR
                     DWREIPIDKVFRDHADSRHVLECTVDVGGTDVSFVGVHVSSKLWYAGPIVHLRSIMRE
                     LPPKDGPAVIAGDFNFWGPGVSRIARGWRRTVRGRTYPAPRPHSQIDHILVNDRVAVE
                     DATVLGDVRSDHLPVRATLSVH"
     gene            complement(3995..5128)
                     /locus_tag="VFX21_10940"
     CDS             complement(3995..5128)
                     /locus_tag="VFX21_10940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5096524.1"
                     /translation="MNFAFSEEQEELRRTVRQFLEAKSPETEVRRLMETQEGYDPAVW
                     KQMGQELGLQGLAIPEEYGGQGFTFIELGIVLEEMGRVLLCAPYYASSVLAANAILNA
                     GTDDQKKELLPGIATGDTIATLAFTEPSGKWDAAGITLEAREGESGRFTLTGEKMFVL
                     DGFTADLIVVVARLAGTSGADGISFFTCRGDADGLTRTALQTMDMTRKQAKLEFNNVE
                     VTPLGSTVDGFAAFSKTLDQAAVALANEMMGGAQFVLDMSVQYAKDRVQFGRPIGSFQ
                     AIKHKCADMLLEVESGKSAAYYASWAAAEDNDELPVVAALSKAYCSEAYFHATAENIQ
                     IHGGIGFTWEHPAHLYFKRAKSSEIFLGDATYHRELLAQRIGI"
     gene            5247..6383
                     /locus_tag="VFX21_10945"
     CDS             5247..6383
                     /locus_tag="VFX21_10945"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HEX5096525.1"
                     /translation="MAVTRAGAESPTTGVRLPRALQVVAAALAAVAVHVFAVWTAVKM
                     QPGLRLREELTDWDGAWYIEIARHGYPSHVLTGSGDGAQSAIAFYPLYPAVLRVVDAL
                     TPLGPAQAALLVAIVAAVGALVAIDRLVTRVCTDDVAWRAVILIAFAPGAIVLSMAYS
                     EGLFMLFVALCLLRLVERRWLAAGAYGALACLTRPSGLAVIAACVAAAYVEWRRTERD
                     AGVVVAPLLAIAGYAALPLYHWVHAGTPLAWWQTEDRGWGMGFDFGWSTAGKMIDVVQ
                     DPGHDFNLLLGTLGVLYMVGGLVALWYWKPPLPVAAYTVVAVGLAVGASQVVSTMRFA
                     MAAFPLAIAYARVLRNNAFVVVVGVSAALMAVLAMAATTTLYTP"
     gene            6380..7213
                     /locus_tag="VFX21_10950"
     CDS             6380..7213
                     /locus_tag="VFX21_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HEX5096526.1"
                     /translation="MIDPMIDVDAILERLGLGARPAPDLDGLARVYLAWCRNVPFDNV
                     VKRVHMASGDTGPIPNGPPDAFFDWYLRHGTGGTCWPSANALHALLVGLGFDARRGSA
                     AMADSKIDGEYAHSHGTTLVRVDGTDHWVDTSMLTDRVLPLARGATTSIPGPVHPLRA
                     EPVGDRWRVWWEPGPRGQEMSCLLLSADTTSEHYLARYEFSRGWSPFNLWVHAVRNTE
                     RGRLTLTNGTRYERRADGGDTRALDVGDRTKVLVEEFGYSEEIVARLPPDEAPPVPSK
                     S"
     gene            7210..>7641
                     /locus_tag="VFX21_10955"
     CDS             7210..>7641
                     /locus_tag="VFX21_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5096527.1"
                     /translation="MTRPAHYETTDVRIDKLVVGPFENNVFVVRDKGSGEAVIIDAAN
                     EHELLLEVSRRTGVRRVLTTHGHFDHIQAVTQMRDAGIDVGVAAEDAKMLPSYDFTIP
                     DDDVITVGGLRLHTIHTPGHTPGSTCFVLEGHPIVFSGDTLF"
BASE COUNT         1126 a         2617 c         2618 g         1280 t
ORIGIN      
        1 gtagaacggc gcacccaggg agtacacccc accgaccgac gacagcaccc agtacccgtt
       61 cgctgacgct gaactcggat gcggggccgg gttcgacggc ggtcggcaca cgtcggtcgg
      121 cagcggcgcg cccgtgtggc ccgcgttctt gtggaacatc ggccacggcg gcgtcttgcc
      181 cggcgtgggg atcgcgtacg actcgatgcg cgtgaagttc tgcggcgtgt cgaagccgct
      241 gacgatcagc ttccagccga gcggcccgaa gtcgcccacc gcgcccgccg attcgtagct
      301 cacgaacgtg ttgacggcag ccatctctgc gccgttcgac ccgttgagca cgaagaacgc
      361 gaagttgttg gcgacgccga cgtcgttgac gccgtcaccg ttcatgtcgg cgatgatcgg
      421 gctcgcgacc ggaccaccgg gcgtcgagtt cacgaagcgc aaaccgctgg cccacagctg
      481 ctggccgtgg ccgttgatgg cgcgcacata gccgtcgttg ctgccggcga ccacgtcggc
      541 cacaccgtcg ttgttgacgt caccgatcgc cggtgagctg ttggtctgcg cgccggtcag
      601 gaccgggaag ccgccttgat cggatccgtc gtggatgttg aaggcgaaga tcttccggcc
      661 ctcgtcgcgg ttgtagtagt tgccgccgcc ggtcacgacc tccatcgaac cgtcgccgtc
      721 gatgtcaccg atcgcggggc tcgagtggaa cacgtcgttg atgcggcgct tccacagctc
      781 gacgacgctc ccgttgcgcc agtcgagcgc gcggaactcg ccgcccgacc agtcgatcgc
      841 accgccggcc atctggtcgg aaccgatgaa gatctcgagg cggccgtcgc cgtcggagtc
      901 gtacagcgcg ggagacgacc acgtggagtc ctcgacgttc accgggaatc cggagagctc
      961 gttgcagtcg cggttgagcg cgtgcacgcg aaggtcccag ccgccgaaca cgatgtcgcg
     1021 gaagccgtca ccgtcgacgt cgccgagggc gggcgtcgaa tagacgcctt ggtcgttgtt
     1081 gccgctccag cccgcggtct cgaagctgca cttcaccgaa ccgtcgcggc ggaacaccac
     1141 gacgccaccc ggcgccttgc gccacgtcga cccacccggg acgacgatct ccggggcacc
     1201 gtcgttgtcg aggtcaccga cgctcggcga accgtcgacc ggcgtgatgt aacccggccg
     1261 gatcacttcg ggctgcggcc agcccgggat gttgtcgccg gtcgccgcgt tcaacacgcg
     1321 gacccaaccg tcctgcgcgc cgtggatgat ctcgggcaca ccgtcaccgg tgacgtcggc
     1381 gatcgtcggt gacgaactgc ggttccaacc gctgaacgac ttcgaccatt gcgcaccgcc
     1441 gatggcggcg gccggcgtcg cggttgcgac gaagggaagc gaagtggcga cgacggcgac
     1501 ggccaacgcc gacgcggtga agatgcgagt gcgcaacaag aagagtcctc gggtcgcgag
     1561 cggcgcagaa ccttgatcct tatcggcaca gtgcggccgg cagtgaagaa cgccacagat
     1621 ccggagccgg ccgaggctcc cggtagccag acggtctgag cggtacgttc ccgcccgtat
     1681 gacggccgtg gacgctgccc cgagccgcgc ggccgggacg gaacgtggca tccgcctcgc
     1741 gctggccggc ctgctcggcc tgctcgtcgt gactgtcgcc catctggtgc tcgcgtacat
     1801 gcggcactgg ctgaacgagg accacacgat cctctggttt gcggcccgcg acctgggcca
     1861 tctcacgttc cctcaaccga gctactacgg cgccgcgtac ttcagcgtgt tcgaggccgc
     1921 accggcggag gcattgcgcc gcgccggcgt gcccctcggc accgcggtct tcgtgagcac
     1981 ggcgacattg atgacgatcg gctgggtgtg gttcgcgttg gtcgcgtggc gacgtggccg
     2041 tttcgcgatg gccgccgccg cgctcgcggt gccggtggtc atcacgaccg agttcctcgt
     2101 ctccgcggac tcgcccggcg gacgcggcgc gggcacgttc tgcgcgatga ccggagtcgc
     2161 gatcttgctg ttcgcgccgg cgcggccggt acccatcgca ctcttcgcgt tgctaggcgg
     2221 cctgggaacg ctgtgggact tcggcagcgc gttgctcgtc gtgccggtcg gcgtctacat
     2281 gctcgcgctg aactggcgac ggtggcgcgt gctggcatgg ggggcgatcg gttccgtcgt
     2341 gccgctcgcg ttcgcgctcg gacaacgcgc gttctacgcc gaccatcccg actacaacgt
     2401 gcatcgtgcg gccggcttca tgccgaagct gtcgatcctg cgcgacacga tccgcgagcc
     2461 ggggcgctac tttgcgtggc tctcgcccga gttgtcgagg acgagcgtgg tgccgatcgt
     2521 tgccggcgcg atcttgctgt tcttcctcgc ccgcacccga cgcgcggaag tgatcctgcc
     2581 cggcgtcgcg ctggtggcga tcgtcctgtt ctcgttgtcg acgccgaagg cgctcgacgg
     2641 gacgccgtcg gtgtacgtcg gctacggccg ctacttcctg tcggtcccgg ccgcgctgtg
     2701 gttcctgggt tgggtgctca ccgaagcgcg ccctccgagc ccgcggttac agcgccggct
     2761 cgtgtacggc atcgccgcgt tcgcggtcgt gacgttcgcg gtgcgactcg tcacctttga
     2821 cgaccgcacc ggtgacatcc ggcgcgtggc ggccgacccg gctcgcgccg gagcacccgt
     2881 cttcgccgcc gacgcggtgg ccgcgcgctg cgatgcggtc gagcacgcag ccgaccgcgc
     2941 cggcgtcgac tatgtcgtgt tcaggggcga ccgcgtcggc gcgtatgcgt gcgccgcgcg
     3001 cgactacggc acgctcgaaa cgatccaccc gatctacgac cggcgcacct ggttgctgca
     3061 cgacgcgaac gaggagaacc gcaccaagtt tctcgtcacc gacgtcgacg aagggtggtg
     3121 catcaacgcc cgcgcgatcg tcgagtcgtg cgagctcgac cccgtcgaca gcacgatcgc
     3181 cgtcgtcacg accgcaccgc agccggtgct cgagacgtgg cggcagctcg gtgaagagat
     3241 ccggccgttc gatcagtgaa ccgaaagcgt cgcgcgcact ggcaagtggt cggagcgaac
     3301 gtccccgagc accgttgcgt cctccaccgc gacgcggtcg ttcacgagga tgtggtcgat
     3361 ctggctgtgc ggcctcggcg cgggatacgt gcggccgcgc acagtgcgcc gccacccgcg
     3421 tgcgatgcgg ctcacacccg gcccccagaa gttgaagtca ccggcgatga ctgcgggacc
     3481 gtccttcggc ggcagctcac gcatgatcga acgcaggtgg acgatcggcc ccgcgtacca
     3541 gagcttcgac gacacgtgga caccgacgaa cgagacgtcg gtgccgccga cgtcgaccgt
     3601 gcactccaac acgtgacgcg agtcggcgtg gtcgcggaac accttgtcga tggggatctc
     3661 acgccagtca cgcgccggca agcggctcaa caccgcgagc tcccatgtgc ccggttcgaa
     3721 gccgcggtac cgcttgtgtg atccgtccgt ctcgtcgaac aaacgactga atgccaccga
     3781 ccggatctcg taggtgtcct cgagcccgtc gagaatcgtc cggccgtcgt cgtaacgaaa
     3841 cgactccggg atgacgacga ggtcggcatc gaagctccgc acgatcggaa cgacgtcgaa
     3901 gcgccgtcgg ttcgtcgaac cgttccgatc gatgccccaa tgcgcgttga accccgcgac
     3961 agtaaacctg gtcagagact cggaattcta agtgctagat accgatccgc tgcgccagca
     4021 gctcgcggtg gtacgtggcg tcgccgagga agatctcgga gctcttcgcg cgcttgaagt
     4081 agaggtgcgc ggggtgctcc cacgtgaagc cgataccgcc gtggatctgg atgttctcgg
     4141 cggtcgcgtg gaagtacgcc tcggagcagt acgccttcga cagcgcggcg acgacgggca
     4201 gttcgtcgtt gtcttccgcg gccgcccacg atgcgtagta cgcggccgac ttgcccgact
     4261 ccacctcgag cagcatgtcg gcgcacttgt gcttgatcgc ctggaacgag ccgatcgggc
     4321 ggccgaactg cacgcggtcc ttggcgtact ggaccgacat gtcgagcacg aactgcgcgc
     4381 cgcccatcat ctcgttggcg agcgcgactg cagcctggtc cagcgtcttc gagaatgcgg
     4441 cgaagccgtc gacggtcgac ccgagcggcg tcacctcgac gttgttgaac tcgagcttcg
     4501 cctgcttgcg ggtcatgtcc atcgtctgca gcgcggtgcg ggtcaggccg tcggcgtcac
     4561 cgcggcacgt gaagaacgag atgccgtcgg caccgctcgt gccggccagg cgcgccacga
     4621 cgacgatgag gtcggcagtg aaaccgtcga gcacgaacat cttctcgccg gtcagcgtga
     4681 accggccgct ttcgccttcg cgcgcttcga gtgtgatgcc ggcggcgtcc cacttgccgc
     4741 tcggttcggt gaacgcgagc gtcgcgatcg tgtcaccggt cgcgatgccg ggcagcagct
     4801 ccttcttctg gtcgtcggtg cccgcgttca agatcgcatt cgcggccagc accgacgacg
     4861 cgtagtacgg cgcgcacagc agcacgcgac ccatctcctc gagcacgatg ccgagctcga
     4921 tgaacgtgaa gccctggccg ccgtactcct cggggatcgc gagaccctgc agaccgagct
     4981 cctggcccat ctgcttccac actgcggggt cgtagccctc ttgggtctcc atcaggcgcc
     5041 ggacttcggt ctccggcgac ttggcctcga ggaactgccg cacggtgcgt cgcagttctt
     5101 cctgttcctc ggagaaagca aagttcactg gtacctctcc tcacgatcga ggctcacgtg
     5161 gagttcgttg ggcggcgcgc aagttacttg accggccggt caggtttcca caccgcgacc
     5221 ggcgccggtc ctacgctcca acccgtatgg ccgtgacgcg cgcaggcgcc gagtcgccga
     5281 ccacgggcgt gcggctgccc cgggcgctcc aggtcgtggc cgcggcactg gcggcggtgg
     5341 cggtccacgt gttcgcggtc tggaccgcgg tcaagatgca gcccggtctg cgcctgcgcg
     5401 aggagctgac cgactgggac ggcgcctggt acatcgagat cgcccgccac ggctacccca
     5461 gtcacgtgct gaccgggtcg ggcgacggcg cccagtcggc gatcgccttc tatccgctgt
     5521 atccggcggt cctgcgggtg gtcgatgcgc tgacgccgct cgggccggcc caggccgcgc
     5581 tgctcgttgc catcgtcgcg gccgttggcg cgctcgtcgc gatcgatcga ctcgtgacgc
     5641 gcgtgtgcac cgacgacgtc gcgtggcggg cggtgatcct catcgcgttc gcgccgggcg
     5701 cgatcgtgtt gtcgatggcg tacagcgaag ggctgttcat gctcttcgtc gcgctgtgtc
     5761 tcctgcgact cgtcgagcgg cgctggctcg ccgccggcgc gtacggcgct ttggcgtgcc
     5821 tcacgcgacc gagcggcttg gcggtgatcg ccgcgtgcgt cgcggcggcg tacgtcgaat
     5881 ggcgccgcac cgaacgcgac gccggcgtcg tcgtcgcacc gttgctcgcg atcgcggggt
     5941 atgccgcgct gccgctgtac cactgggtgc acgcgggcac gccgctcgcc tggtggcaga
     6001 ccgaagatcg cggctggggc atggggttcg acttcggctg gtcgaccgcc ggcaagatga
     6061 tcgacgtcgt gcaggatccg ggccacgact tcaacctgct gttgggcaca ctcggcgtgc
     6121 tgtacatggt cggcgggctc gtggcgctgt ggtattggaa gccgccgtta cccgtggccg
     6181 cgtacacggt tgtcgcagtg gggctcgcgg tgggcgcgtc acaggtcgtc tcgacgatgc
     6241 ggttcgcgat ggccgccttc ccgctcgcga tcgcctacgc gcgcgtcctg cgcaacaacg
     6301 cgttcgtcgt cgtggtcggc gtgagcgcgg cgctgatggc ggtcctggcg atggccgcga
     6361 cgaccacgct ctacacgcca tgatcgatcc gatgatcgac gtcgatgcga tcctcgagcg
     6421 actcggcctc ggcgcccggc ccgcgcccga cctcgacggt ctcgctcgcg tatatctcgc
     6481 gtggtgccgc aatgtgccgt tcgacaacgt ggtgaagcga gttcacatgg cgagcggtga
     6541 taccggaccg atcccgaacg ggccgccgga cgcgttcttc gactggtacc tgcggcacgg
     6601 caccggcggc acgtgctggc cgagcgcgaa cgcactccac gcgctgctcg tcgggctcgg
     6661 gttcgacgcg cggcgcggct cggcggcgat ggccgactcg aagatcgacg gcgaatacgc
     6721 gcattcgcac ggcacgacgc tggtccgcgt cgacggcacc gaccactggg tcgacacgtc
     6781 gatgctcacc gaccgggtgc tgccgctcgc gcgcggcgcc acgacgtcca ttcccggtcc
     6841 ggtgcatccg ctgcgcgcgg agccggtcgg tgaccggtgg cgcgtctggt gggaaccggg
     6901 cccgcgcggc caggagatga gctgcctgct cctgtcggcg gacacgacga gcgagcacta
     6961 cctcgcgcgc tacgagttct cgcgtggatg gagcccgttc aacctgtggg tccacgccgt
     7021 gcgcaacacc gagcgcgggc gcctgaccct gaccaacggc acgcgctacg agcggcgggc
     7081 cgacggcggc gacacgcgcg cgctcgacgt cggcgaccgg acgaaggtgc tggtcgagga
     7141 gttcggctac tccgaggaga tcgtggcgag gcttcctccc gacgaggcgc cgccggtacc
     7201 ctcgaaatca tgacgcggcc cgcgcactac gagacgacgg acgtccgcat cgacaagctc
     7261 gtcgtcgggc cgttcgagaa caacgtgttc gtcgtgcgcg acaagggcag cggcgaggcc
     7321 gtgatcatcg acgccgccaa cgagcacgaa ctgctgctcg aagtgtcgcg ccgcacgggt
     7381 gtgcggcgcg tgctgacgac gcacggtcac ttcgaccaca tccaggccgt gacgcagatg
     7441 cgcgacgcgg gcatcgacgt cggcgtggcc gccgaggacg cgaagatgct gccctcgtac
     7501 gacttcacga tacccgacga cgacgtcatt acggtcggcg ggctgcgttt gcacacgatc
     7561 cacacgccgg gccacacacc cggttcgacg tgcttcgtgc tcgagggcca tccgatcgtg
     7621 ttcagcggcg acacgttgtt c
//
LOCUS       DASUGA010000551         2257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_47409, whole genome shotgun sequence.
ACCESSION   DASUGA010000551 DASUGA010000000
VERSION     DASUGA010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47409"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VFX21_10960"
     CDS             complement(<1..839)
                     /locus_tag="VFX21_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5096528.1"
                     /translation="MPSPFVASQPDFFAVGPAAAPAAIDMGAGVWCSPGMSNTYLVVT
                     DEGRVVVNTGMWFEAKTHKRNFDAVSDAPTRYIVLTQSHTDHVGGVDRFRGDGTQLVA
                     QADNAACQADDQRIHGFRIRRSLPFFAPVMGQPGFVRGDDAEIPPRPKVVVPDVLFED
                     SYAFELGGRRFELLSVPGGETIDSLIVWLPDERIALVGNMFSALFGHFPNLVTMRGDR
                     LRFALSFVSSVQRVIDLEPEVLLLGHHGPVRGAALIRSECERIRDAVQYVHDATVEAM
                     NRG"
     gene            1165..2139
                     /locus_tag="VFX21_10965"
     CDS             1165..2139
                     /locus_tag="VFX21_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013225050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HEX5096529.1"
                     /translation="MTQLLASSGSSSENFVDLDVPITAWVALVALILVMLGVDLALHR
                     GDKEPTPKHALFESLTWVTIGLSFAGVLALIFNRQASGEYLAGYLVEKSLSVDNVFVW
                     AVIFSTFAIPLKYQHRVLFWGIFGALVLRAIFIFVGVSLLNAAWWILLVFGAFLVFTG
                     ARVLRHREDEGTHGHDRAVNMLQRFMPVSKEFDGHNFFTHIDGRKAATPLLAALVVVE
                     TTDVVFAVDSVPAVLAVSNEPFLVFAANAFAILGLRAAYFLLAGWKERLHYLGHGLGL
                     ILIYVGLKMMVSHWWHAPTWVSLGIIAALLAIAVIASLVREKRVAAAA"
BASE COUNT          349 a          736 c          763 g          409 t
ORIGIN      
        1 tcacctcggt tcatcgcctc gacggtcgcg tcgtgcacgt actgcaccgc gtcacggatc
       61 cgctcgcact cggaccggat cagtgctgcg ccccgaacgg gcccgtggtg accgagcagg
      121 agcacttcgg gttcgaggtc gatcacgcgc tgcacgctcg agacgaacga gagcgcgaag
      181 cggagccggt cgccgcgcat cgtcacgagg ttcgggaagt ggccgaacag ggccgagaac
      241 atgttgccga cgagcgcgat gcgttcgtcg gggagccaga cgatgagcga gtcgatggtc
      301 tcgccgccgg gcaccgacaa cagctcgaag cgccggccgc cgagctcgaa tgcgtacgag
      361 tcctcgaaca gcacgtcggg cacgacgacc ttcggccgcg gcgggatctc ggcgtcgtcg
      421 ccgcgcacga agccgggctg gcccatgacc ggcgcgaaga aggggagcga gcgacggatg
      481 cggaagccgt ggatgcgttg gtcgtcggcc tggcacgcgg cgttgtcggc ctgcgcgacg
      541 agctgcgtgc cgtcgccacg gaaccggtcg acgccgccga cgtgatcggt gtggctctgc
      601 gtgaggacga tgtagcgggt cggcgcgtcg ctcaccgcgt cgaagttccg cttgtgcgtc
      661 ttggcctcga accacatgcc ggtgttgacg acgacccggc cctcgtcggt gacgacgaga
      721 tacgtgttcg acatcccggg tgagcaccac acgccggcac ccatgtcgat cgcggccggt
      781 gccgcggcgg gcccgacggc gaagaagtcg ggttgggatg caacgaacgg gctcggcacg
      841 cctcgatctt cgcgtgccgg gcgtcggcaa gtgctccgtc cggtgacgtt gtcgatgttt
      901 acggcgagtc aggggcgtcg ctcgcaagga cgcacgacga aggaagagtg ggcctctttt
      961 gaggaggagg acgccgcgag cggcgtcact ggccggcgta aacgcggcaa acgttgccgg
     1021 tctgggcgct aggctgctgc gccagaaggg gagtacctcg caacggcggt gtcgtcagta
     1081 cgggcctcgg ccccggcacc accggcctcc acggctgggg gagaccttcg aatcacgtgc
     1141 tgctcgtgca ttcgaaaggc ttccatgacc cagctcctcg cttcctccgg ctcgtcgagc
     1201 gagaacttcg tcgacctcga cgtgccgatc accgcctggg tcgcgctggt cgccctcatc
     1261 ctcgtcatgc tcggcgtcga cctcgcgcta caccgcggcg acaaggagcc gacccccaag
     1321 cacgcgctgt tcgaatcgct cacatgggtg acgatcgggc tcagcttcgc gggcgtcctt
     1381 gccctcatct tcaaccggca ggcctccggc gaatatctgg ccggttacct cgtcgagaag
     1441 tcgctgtccg tcgacaacgt cttcgtctgg gccgtcatct tctcgacctt cgcgatcccg
     1501 ctcaagtacc agcaccgcgt gttgttctgg ggcatcttcg gtgcgctcgt gctgcgcgcc
     1561 atcttcatct tcgtcggtgt ctcactgctc aacgccgcct ggtggatcct gctcgtcttc
     1621 ggtgcgtttc tcgtgttcac cggcgcgcgg gtcttgcggc accgcgaaga cgaagggacg
     1681 cacggtcacg accgcgcggt caacatgctg caacggttca tgccggtgtc gaaggagttc
     1741 gacggtcaca acttcttcac ccacatcgac ggccggaagg cggcgacgcc gttgctggcc
     1801 gcgctcgtcg tcgtcgagac caccgacgtc gtgttcgcgg tcgactccgt cccggcggtg
     1861 ctcgcggtca gcaacgagcc cttcctggtg ttcgccgcca acgcgttcgc catcctcggc
     1921 ctccgggcgg cgtattttct gctcgccggc tggaaggaac ggctgcacta cttgggccac
     1981 gggctcggcc tgatcctcat ctacgtcggt ctgaagatga tggtcagcca ctggtggcac
     2041 gcgccgacgt gggtgtcgct cggcatcatc gccgcgctct tggcgatcgc agtgatcgca
     2101 tcgctggtgc gagaaaagcg agtcgcagca gcggcgtagc catccagcgc actgcatcat
     2161 gaacacgaga tggatcaatg ttcgctcgac gttggagcgt tagggtggcg tcggtgcaac
     2221 cgagcatgtc gctcgttggg ttgccttgtg tcaggag
//
LOCUS       DASUGA010000552         2210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_602389, whole genome shotgun sequence.
ACCESSION   DASUGA010000552 DASUGA010000000
VERSION     DASUGA010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602389"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1907)
                     /locus_tag="VFX21_10970"
     CDS             complement(<1..1907)
                     /locus_tag="VFX21_10970"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5096530.1"
                     /translation="MDAAESDAHRRLRKLWVPTVLTGATFAVLYTLDLIGDLPIFVLL
                     GLIVVAGGLGELAHARWTEDMSPARLYFLTSPQVLSVAVIIYAIGWGPMLAVGYVFVV
                     AGALDEIGSRAWRPMLMWTAIGMVLGQVAVATEAVPSYLRPSYSHGLAVLSFLGVAFV
                     LYALGTKTEENERATAALARRNADFRQLFAENPQPMWVFDLETLAFLEVNAAAISHYG
                     YSREEFLARRINDLRPDGEAPGILDAIEQKRSLGYGTGSARHRLKDGRIIEVEASGHH
                     LMFQGREAGLVAIQDVTEQKRLENELRHQAFHDSLTDLANRALFADRIDQAIRRHRRD
                     DRFGAVLLIDLDRFKTVNDSLGHTVGDALLMRFAHLIEGTLRAADTAARLGGDEFGVL
                     LEDIDDVEDATKAAERILDALAEPIDVDGVEIFVHASIGVTITGIDGQSAEELLRNAD
                     AAMYGAKADGRGRMRTYEPAMHQAALNRLELEADLRRAVAANELVVHYQPIVSIAERK
                     VSALEALVRWHHPVRGLVQPDEFIPVAEDTGLIVDVGRFVLREACHQASAWRAAGNDF
                     TIAVNLSARQLSAIDLVSDVANALETSGLLPSALTLEITESVLIKDPETAVRRLRELK
                     ALGVRLAIDDFG"
BASE COUNT          380 a          737 c          724 g          369 t
ORIGIN      
        1 ccgaagtcgt cgatcgcgag gcggacgccg agcgccttca gctcgcgcaa ccgccggacc
       61 gcggtttcgg gatccttgat cagcacgctc tcggtgatct ccagcgtcag cgcgctcggc
      121 aacaatcccg acgtctcgag cgcgttcgcg acgtcgctca ccagatcgat cgcgctcaac
      181 tggcgagccg agaggttgac cgcaatcgtg aagtcgttgc cggccgctct ccatgcgctc
      241 gcctggtgac acgcttcccg cagcacgaag cgcccgacat ccacgatgag cccggtgtcc
      301 tcggcaaccg gaatgaactc gtctggctgg acgaggccgc gcaccgggtg atgccatcgc
      361 acgagcgctt ccagcgcgct caccttgcgt tccgcgatcg aaacgatcgg ttggtagtgc
      421 acgacgagct cgttggcggc aaccgcgcgg cgcagatccg cttcgagctc gagccggttc
      481 agtgccgctt gatgcatcgc cggttcgtac gttcgcatgc ggccgcgccc gtccgccttg
      541 gcgccgtaca tcgccgcgtc ggcgttgcgc agcaactcct ccgcgctctg accgtcgatg
      601 cccgtgatcg tcacaccgat gctcgcgtgc acgaagatct cgacaccgtc gacgtcgatc
      661 ggctccgcaa gtgcgtcgag aatgcgttcc gccgccttcg tcgcgtcttc gacgtcgtcg
      721 atgtcttcga ggagcacgcc gaactcgtcg ccgccgagcc gcgccgcggt gtcggccgcg
      781 cgcagcgtgc cctcgatcag gtgcgcgaag cgcatgagca gcgcgtcgcc gacggtgtga
      841 ccgagactgt cgttcaccgt cttgaaccgg tcgaggtcga tgagcagcac cgcgccgaac
      901 cggtcgtcgc ggcggtgccg tcggatcgcc tggtcgatgc ggtcggcgaa cagcgcacgg
      961 ttggccaggt cggtcagcga gtcgtggaac gcctggtggc ggagctcgtt ctcgagacgc
     1021 ttctgctcgg tgacgtcttg gatggcaacg aggcccgctt cccgaccctg gaacatgagg
     1081 tggtgaccgg acgcctcgac ctcgatgatg cgcccgtcct tcaggcggtg gcgcgcggag
     1141 ccggtgccgt agccgagact gcgcttctgt tcgatcgcgt cgaggatgcc cggcgcctca
     1201 ccatccgggc gaaggtcgtt gatgcggcgc gcaaggaact cttcccggga gtagccgtag
     1261 tggctgattg ccgccgcgtt gacttccagg aaggccagcg tctcgaggtc gaacacccac
     1321 atcggttgcg ggttctccgc gaacagctgc cggaagtcgg cgttgcggcg tgccagcgca
     1381 gcggttgcgc gttcattttc ctcggtcttc gtgccgagcg cgtacaagac gaacgcaacg
     1441 ccgaggaacg acagcaccgc gaggccgtgc gagtagctcg gtctcaagta actcggcacg
     1501 gcctcggtcg cgacggcaac ctgaccgagc accatgccga tcgcagtcca catcagcatc
     1561 ggtcgccagg cgcgcgagcc gatctcgtcc agcgcgcccg caacgacgaa cacatagccg
     1621 acggcaagca tcgggcccca cccgatcgcg taaatgatga ccgcgacgga caagacctgc
     1681 ggagacgtga ggaagtacag ccgcgccggg gacatgtctt cggtccagcg cgcgtgcgcg
     1741 agctcgccga gcccgccggc gacgacgatg agaccgagca ggacgaagat cggaaggtca
     1801 ccgatgaggt cgagggtgta caagacggcg aatgtcgcgc cggtcaacac ggtcgggacc
     1861 cacagcttgc gcaatcgccg gtgcgcgtcc gattcggcgg cgtccacgga acgcataccg
     1921 ctctgtcggc gctgctcgtg gtcttgtgaa ggccctgatg acgaactgag acccttttct
     1981 gggggccagg aggccacccg ggcaaaagaa ccggtcgaag tagtcccgac cgttcagttt
     2041 gtccgaattt ctacgcacat gtgaccagga cggccatgtc cggcggcgag ccgcccgacc
     2101 cgagaccgca tagagcagtg gcgtctcgtc gccggtgacg gtcacttggt aagaatctcc
     2161 cttcgtggct gacgacgaac acgatcacga tcacgacgat cacgatcacg
//
LOCUS       DASUGA010000553         2040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1489221, whole genome shotgun sequence.
ACCESSION   DASUGA010000553 DASUGA010000000
VERSION     DASUGA010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489221"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..1219
                     /locus_tag="VFX21_10975"
     CDS             5..1219
                     /locus_tag="VFX21_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HEX5096531.1"
                     /translation="MDVVGNNIANVNTTGYKSSNVLFEDLLSQTLRGAGSPVSGTQGG
                     TNPAQVGLGVRLTGVTLNMAQGATQLTGRATDFSLQGDGFYAINSGGTRAYTRNGSFS
                     LDGLGQLVTQDGGFVLGWQASVTGTVDTNASIGNIRIPVGQVIAPVTTSSIDIGGNLP
                     SDAATGTNINVSIDVYNSLGAAVPLRAEYRKIANVAPDVNWEVRVYDAANNVIHAATA
                     VTFGPTGTRSTPNVTITQAELNAIAGTSGTWGAGGITLDMGAATDADRLTGAATLNSA
                     AALSQDGSPIGSLVSFSVGSDGLITGVFSNGRNQALGQLAIATFANPVGLEKAGNSLY
                     RSSINSGAAQIGIAGQAGRGTLSGGTLEMSNVDLAAEFTNLIVAQRGFQANSRIITAS
                     DEILQDLVNLKR"
     gene            1333..1587
                     /locus_tag="VFX21_10980"
     CDS             1333..1587
                     /locus_tag="VFX21_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HEX5096532.1"
                     /translation="MITVHRLNGPAFSLNHDLIERVEATPDTVITLVDDTKYVVTESV
                     DEIVARVRDAKAAVVAHAHLLEHRFSGGPTLRVVPGGDEE"
     gene            1591..>2040
                     /locus_tag="VFX21_10985"
     CDS             1591..>2040
                     /locus_tag="VFX21_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096533.1"
                     /translation="MAKKKAGEEDEDGGGSAKGLIIKVVVALVVGAFVAKTFFLKPPP
                     PTEAALKAKAKGEFVDMAFKCAASNEVAYDAAAVDAEFEHLYEEEHAAAPTTVAAAAE
                     APAEAPQFAGEVGGGGLGMKLASARPASGGAEGAVATGPALLEVDATT"
BASE COUNT          359 a          685 c          714 g          282 t
ORIGIN      
        1 gttcatggac gtggtgggga acaacatcgc caacgtcaac acgaccggtt acaagtcgag
       61 caacgtcttg ttcgaggacc tgctcagcca gacgctgcgc ggcgccggct caccggtttc
      121 gggcacccaa ggtggtacca accccgcgca ggtcggtctc ggtgtgcgcc tcacgggcgt
      181 gacgctgaac atggcgcagg gcgcgacgca gttgacgggt cgggccaccg acttctcgct
      241 gcagggcgac ggcttctacg ccatcaactc cggtgggacg cgcgcgtaca cccggaacgg
      301 cagcttctcg ctcgacggtc tcggccagct cgtcacccag gacggtggat tcgtgctcgg
      361 gtggcaggcg agtgtgacag gaaccgtcga caccaacgcc agcatcggca acatccgcat
      421 cccggtcggc caggtcatcg cgccggtcac gacgtcgtcg atcgacatcg gtggcaactt
      481 gccgtcggac gcggcgacgg gcacgaacat caacgtctcg atcgacgtgt acaacagcct
      541 cggtgccgcg gtgccgctgc gcgccgagta ccgcaagatc gcgaacgtcg caccggacgt
      601 caactgggaa gtgcgcgtgt acgacgctgc gaacaacgtt atccacgccg cgaccgcggt
      661 gacgttcgga ccgacgggta cgcgcagcac gccgaacgtg acgatcacgc aagccgagct
      721 caacgccatc gcgggtacga gcggcacgtg gggtgcgggc ggcatcacgc tcgacatggg
      781 tgccgcgacc gacgccgacc gcctgaccgg cgccgcgacg ttgaacagtg cggcggcact
      841 gtcgcaggac ggctcgccga tcggatcgct cgtgagcttc agcgtcggtt ccgacggcct
      901 gatcacgggt gtgttctcga acggccgcaa ccaggccctc gggcagctcg caatcgcgac
      961 cttcgccaac ccggtcggtc tcgagaaggc gggcaactcg ctgtaccgct cgtcgatcaa
     1021 ctcgggcgcg gcccagatcg gcatcgccgg ccaggcgggc cggggcacgc tctcgggcgg
     1081 cacgctcgag atgtcgaacg tcgacctggc agccgagttc accaacctca tcgtggccca
     1141 gcgcgggttc caggccaact cccgcatcat cacggcctcc gacgagatcc tgcaggacct
     1201 cgtcaacctg aagcggtagg agcggtcggg agagagatgg cggttagtcc tcacgtgacc
     1261 ctcgtccccg gccgataggg aggggacgag ggtccggagg gacccggcaa ctcgaggtcc
     1321 aaggacggat cgatgatcac cgtgcaccgg ctcaacgggc cggccttctc gctgaaccac
     1381 gacctgatcg aacgggtcga ggcgacgccc gacaccgtca tcacgctcgt cgacgacacg
     1441 aagtacgtcg tcaccgagag cgtcgacgag atcgtcgcgc gcgtgcggga cgcgaaggcg
     1501 gccgtggtcg cgcatgcgca tctcttggag caccgattca gcggcggacc gaccttgcgc
     1561 gtcgtgcccg gcggagacga ggagtaaccg gtggccaaga agaaggccgg cgaggaggac
     1621 gaagacggcg gcggcagcgc caagggcctg atcatcaagg tcgttgtcgc gctggtcgtc
     1681 ggtgcgttcg tcgccaagac gttcttcctc aagccgccgc cgcccaccga agccgcgttg
     1741 aaggcgaagg ccaagggcga attcgtcgac atggcgttca agtgcgcggc gtcgaacgag
     1801 gtcgcgtacg acgcggccgc ggtcgacgcc gagttcgagc acctctacga ggaagagcac
     1861 gccgccgcgc cgaccacggt cgcggccgcg gccgaggcgc cggcggaggc gccccagttc
     1921 gccggcgagg tcggcggcgg tgggctcggc atgaaactcg cgtcggcccg ccccgcgagc
     1981 ggcggtgcgg agggtgcggt cgcgacgggc ccggccctgc tcgaggtcga cgcgaccacg
//
LOCUS       DASUGA010000554         2692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_447714, whole genome shotgun sequence.
ACCESSION   DASUGA010000554 DASUGA010000000
VERSION     DASUGA010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447714"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VFX21_10990"
     CDS             <1..734
                     /locus_tag="VFX21_10990"
                     /inference="COORDINATES: protein motif:HMM:NF018657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HEX5096534.1"
                     /translation="DRARDAARMVASLRRQALVADAAHSDVLGNRSLRRHFEVGECSL
                     PALREAASRFGGSVNDAYVTALAAALGRYHERYGSDVSELRLAMPISTRGRGDTATNA
                     FVPARVLVPIQPADNMRELFGAVRERLLHTKGEAVINAYEGLAGIAGLLPTAMLVAFT
                     RNQARTIDFAASNLRGSPVPLYLAGQRIVGNYPFGPRTATALNVTMLGYCDDLHLGFN
                     IDPAAIVDIDGLMVDIADSFDALLQ"
     gene            complement(742..1092)
                     /locus_tag="VFX21_10995"
     CDS             complement(742..1092)
                     /locus_tag="VFX21_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096535.1"
                     /translation="MAPGLRFIDEDGAEVVLDSDEAVALLGITRGLDAATVSACPGCR
                     SRVLAAVALVDLLDDSAPHARAGELVELADEAPTLHIYVVDDATRCAHRRWRDPLAAE
                     WQDVIEVSGPHARP"
     gene            1187..2179
                     /locus_tag="VFX21_11000"
     CDS             1187..2179
                     /locus_tag="VFX21_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEX5096536.1"
                     /translation="MRAVTIADGALHIAERPIPEPAAGQVRVRVQGAGINRADLIQRL
                     GLYPAPPGSPPDIPGLEFAGVVDACGDGVTQFTAGDHVFGIAGGGAQAEYLVIPAAQC
                     ARVPDGLDLVAMGGVPEAFVTAHDALVTWAEIRAGEWMLVHAVGSGVGTAALQLARAL
                     GARVIGTARTPDKLERCRDLGLARGIVPPRTTDGALDVPTLAAQLQEETGGGVDVTLD
                     LVGGDYVEADLHAAALKGRIVVIGTLAGGSARLPLLSLMSKRLSLFGTVLRARTDAEK
                     GDAVAAFARDVVPFLASGTLDPVVDRVFPMAEAEAAYALVESDTTFGKVILDCS"
     gene            complement(2176..>2692)
                     /locus_tag="VFX21_11005"
     CDS             complement(2176..>2692)
                     /locus_tag="VFX21_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096537.1"
                     /translation="PEETTAPTRTPEATTATTAPCPPLDGATQDKTETTSSSGYGNLT
                     EVAITTGDCTDAVAFTFTATGDGPDYTIEYQPGPFTMDASGDPVDVDGAAFLTVRFTP
                     AYGFNFDTGQATYNGPDSVTSPGAFFVQEVAKTGDFEGVVSWVIGVDQARDYAVDVTG
                     TTTKVITISIR"
BASE COUNT          364 a          967 c          952 g          409 t
ORIGIN      
        1 ccgaccgcgc ccgtgacgcg gcgcggatgg tcgcgtcgct gcggcgccag gccttggtcg
       61 cggatgctgc gcactccgac gtgctcggca accgctcgct gcgccgccac ttcgaggtcg
      121 gtgagtgctc gctccccgca ctgcgcgaag cggcgtcccg gttcggcggc agcgtcaacg
      181 atgcctacgt caccgcgctc gcggccgcac tgggccgcta ccacgagcgc tacggcagcg
      241 acgtgtccga gctgcggttg gcgatgccga tcagtacccg tggccgcggt gacacggcga
      301 cgaacgcctt cgtaccggcg cgcgtgctcg tcccgatcca gcctgccgac aacatgcgcg
      361 agctcttcgg cgcggtgcgc gaacgcctgc tgcacacgaa gggcgaggcg gtgatcaacg
      421 cgtacgaagg cctcgccggc atcgccggcc tcctgccgac cgcgatgctg gtcgcgttca
      481 cccgcaacca ggcgcgcacg atcgacttcg cggcatcgaa cctgcgcggg agtccggtac
      541 ccctgtacct ggccggccag cgcatcgtcg gcaactaccc gttcggcccg cgtaccgcca
      601 ccgcgctcaa cgtgacgatg ctcggctact gcgacgacct gcacctcggg ttcaacatcg
      661 atcccgcggc gatcgtcgac atcgacggct tgatggtcga catcgccgac tcgttcgacg
      721 cgctgttgca gtagccggcg gctacgggcg cgcgtggggg cccgacacct cgatgacgtc
      781 ctgccactcc gcggcgagcg ggtcacgcca gcggcgatgc gcgcaccggg tcgcgtcgtc
      841 gacgacgtag atgtgcagcg tgggcgcttc gtccgcgagc tcgaccagct ccccggcgcg
      901 cgcgtgcggc gcggagtcgt cgagcaggtc gacgagcgcg accgcggcga gcacacgcga
      961 gcggcaacca gggcatgcgg acaccgtcgc ggcgtcgagc ccacgcgtga tgccgagcag
     1021 ggcgacagcc tcgtcactgt cgagcacgac ctcggccccg tcctcgtcga tgaaccggag
     1081 cccgggcgcc acggggtcac ggtaccgccc ggtgggcaga acgaaatcgg ggcccgctcc
     1141 gaggcagggc ctgggtcccc gaaggccaga taggttgcgc tggttgatgc gagcagtcac
     1201 tatcgccgac ggcgcgctgc acatcgccga gcgcccgata cccgagcccg cggccggaca
     1261 ggtgcgcgtg cgcgtccagg gtgcgggcat caatcgggcc gatctcatcc agcggctcgg
     1321 cctctatccc gcgccgccgg gctcgccgcc cgacatcccg ggcctcgagt tcgcgggggt
     1381 cgtcgacgcg tgcggcgacg gcgtcacgca gttcacggcc ggcgaccacg tcttcggtat
     1441 cgcgggcggc ggcgcgcagg ccgagtacct cgtgattccc gccgcgcagt gtgcgcgcgt
     1501 ccccgacggg ctcgacctcg tggcgatggg aggcgtgccc gaggcattcg tgaccgcgca
     1561 cgacgcgctg gtcacgtggg cggagatccg cgcgggcgag tggatgctcg tgcacgcggt
     1621 cggcagcggc gtcggcaccg ccgcgctgca gttggcgcgt gcactcggcg cgcgcgtcat
     1681 cggcacggcc cgcacccccg acaagctcga acgctgccgc gacctcgggc tcgcgcgcgg
     1741 catcgtgccg ccccgcacca cggacggtgc gctcgacgtg cccacgctcg ccgcgcagct
     1801 ccaggaggag acgggcggcg gtgtcgacgt caccctcgac ctcgtcggcg gtgactacgt
     1861 cgaagccgac ctgcacgcgg ccgcgctgaa gggccgcatc gtcgtcatcg gcacgctggc
     1921 gggcggttcc gcccggctcc cgttgttgtc gctgatgagc aagcggctct cgctcttcgg
     1981 cacggtgctg cgggcccgca ccgacgcgga gaagggcgac gcggttgcgg ctttcgcgcg
     2041 cgacgtcgtg cccttcttgg ccagcgggac gctcgatccc gtggtcgacc gcgtgttccc
     2101 gatggccgag gccgaagctg cgtacgcgct cgtcgagtcc gacacgacgt tcggcaaggt
     2161 gatcctcgac tgcagttagc ggatcgagat cgtgatcacc ttggtggtcg tgcccgtgac
     2221 gtcgaccgcg tagtcgcgcg cttggtcgac accgatgacc cagctcacga cgccttcgaa
     2281 gtcgccggtc ttcgcgactt cctgcacgaa gaacgcgccg ggcgacgtca ccgagtcggg
     2341 accgttgtag gtcgcctgac cggtgtcgaa gttgaagccg tacgcaggcg tgaagcgaac
     2401 ggtgaggaac gcggcaccgt cgacgtcgac cgggtcccca ctcgcgtcca tcgtgaacgg
     2461 cccgggctgg tactcgatcg tgtagtccgg tccgtcgccc gtcgccgtga acgtgaacgc
     2521 caccgcgtcg gtgcaatcgc cggtcgtgat tgcgacttcg gtcaaattcc cgtagcccga
     2581 ggacgacgtc gtctccgtct tgtcctgtgt cgcgccgtcg agcggcgggc atggtgccgt
     2641 ggtcgcggtg gtcgcctcgg gcgtcctcgt cggcgcagtg gtctcctccg gc
//
LOCUS       DASUGA010000555         4801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1862722, whole genome shotgun sequence.
ACCESSION   DASUGA010000555 DASUGA010000000
VERSION     DASUGA010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1862722"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VFX21_11010"
     CDS             complement(<1..801)
                     /locus_tag="VFX21_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX5096538.1"
                     /translation="MRALQLTAWGEPPELRDVPVPEPAPGQVLVRVAGAGACHSDLHL
                     LEAPDGLIPYPLPFTLGHENTGWIAALGAGVVPVDGLRDGDPVAVYGPWGCGWCRACR
                     TSAENICERKPLGEAIGGGIGADGGMAEYMLVPSPRLLVPLGDLDPVEAAPLGDAALT
                     PYHAIAPSRGDLVPGTTAVVIGAGGLGHMAIQLLRATTPARVIAIDKDEQKLSLARDM
                     GADDTINASDDAAARVKAGTRGLGAELVIDLVGADDTLGLGVQCLRARG"
     gene            860..3262
                     /locus_tag="VFX21_11015"
     CDS             860..3262
                     /locus_tag="VFX21_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008747233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX5096539.1"
                     /translation="MTDVVARSAGLTAAEVAERVARGDVNTADERTSRPLSEIIRANV
                     FTRFNAILGTMLVLIIIVGPFQDALFGVVLVANTLIGIVQEWRAKRTLDRLAVLNAPQ
                     ARVVRDGQVTEIAVDGVVLDDLLELRTGDQVPADGRVVEEDGLEIDESLLTGESEPVA
                     KDADDEVLSGSIVVAGRGRCEVTRVGADSYARRLATEARRFTLVRSELMDGINLILRY
                     VTYAIFPTAALLALSQFEAHDGWREAMAGVVAGVVAMVPEGLVLLTSLAFAIAAVTLA
                     RRQVLVQELPAVEGLARVDVVCLDKTGTLTDGNIAFDRVEAFDADVDPAAALGALAAD
                     ENRNATALALAEAFDAPSGWTRTATVPFSSARKWSAACFDGKGTWILGAPENVWAAAD
                     DPVRRRADELASGGQRVLLLTRSDAPITAEVLPPDMQAVALVLFEEQIRPDAADTLAY
                     FAQQGVSLKVISGDNPRTVGAVASRVGLPGAHEPVDARELPEDGDELAGVLEESSVFG
                     RVTPHQKRAIVGALQSRGHVVAMTGDGVNDALALKDADIGIAMGNGAPATRAVAQLVL
                     MDGRFATMPGVVAEGRRVIANIERVANLFVTKTVYAMLLAIAVGVARWEYPFLPRHLT
                     VVSSLTIGIPAFFLALSPNTQRYIPGFVVRVLKFAVPCGFVAAAATFGSYAAARDIAH
                     VDLVEQRTTATLTLLVIGLWVLGILARPITPFRGGLLAAMIACVVGVITIPVFRDFYE
                     FDMPEGAALVVCAVVSVVACALIELGWIFSQHRKEPDARTPRLALRNPAARAAATASR
                     DV"
     gene            complement(3240..4214)
                     /gene="corA"
                     /locus_tag="VFX21_11020"
     CDS             complement(3240..4214)
                     /gene="corA"
                     /locus_tag="VFX21_11020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEX5096540.1"
                     /translation="MRAWSFRRSGTETETVARDGIASVAADGDSLLWVDLTNPTDDDV
                     AFVVEALKIHDLTAEDLTRGNQRTKLDRFNDHFHVALYDDVLRHDGLHSTEVHIVFRE
                     GWLLSVRRAPGGIDPELIDEALRRFERRRGEDGADDEGFVLWAILDVVVDRYFDVTDA
                     VDEIIDEIEELVFNERAEGIPQKLFVLRRGLVRFRRQVGPLREVLNELLRREVSCITE
                     PALVRLQDVLDHVLRVLDLIESQRELLTGLLEGQLAVMSNRTNRVMKLTSSWGAILIV
                     ATLITGVYGMNFEHMPELTWYYGYPIALGLIGVVTVALYVYFKRRDWL"
     gene            complement(4237..>4801)
                     /locus_tag="VFX21_11025"
     CDS             complement(4237..>4801)
                     /locus_tag="VFX21_11025"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX5096541.1"
                     /translation="IAMSRGSRGYWIAFTDARTYAFSPSSAAPTCGPAGASRSDAAAR
                     DFFDRLNAERAARGLPALGWDPGLAQYATDWSRDMSVNGFRHSNLGNLLNGGRFGLVG
                     ENIASGSAGVSAGGLHVAWMHSDGHRSNMLSPGFDLVGIGVYCAPNGSMFATTSFGRT
                     MGAGPAPSAGTPPVDPVVRPDPGSSTC"
BASE COUNT          712 a         1616 c         1727 g          746 t
ORIGIN      
        1 gccgcgggca cgcaggcatt gcacgccgag cccgagtgtg tcgtcggcac cgacgagatc
       61 gatgacgagc tctgcgccga ggccgcgcgt gcccgccttc acgcgcgccg cggcgtcgtc
      121 ggacgcgttg attgtgtcgt cggcgcccat gtcgcgcgcg agcgagagct tctgttcgtc
      181 cttgtcgatc gcgatgactc gggccggtgt cgtcgcgcgc aggagctgga tcgccatgtg
      241 cccgaggccg ccggcgccga tgacgacggc cgtcgtgccc ggcaccaagt cgccccgtga
      301 cggcgcgatc gcgtggtacg gcgtgagtgc ggcatcaccg agtggtgcgg cctccaccgg
      361 gtcgagatcc ccgagcggca cgagcagccg cggcgacggc acgagcatgt actcggccat
      421 gccgccgtcc gctccgatgc cgccaccgat cgcttcgcct aagggtttcc gttcgcaaat
      481 gttctcggcc gacgtgcggc acgcgcgaca ccagccgcac ccccacggtc cgtacaccgc
      541 gaccggatca ccgtctcgca gcccgtcgac gggcacgacg ccggcaccga gcgcggcgat
      601 ccaacccgtg ttctcgtgcc cgagcgtgaa cggcagcggg taggggatga gcccgtcggg
      661 cgcttccaac aagtgcaggt cggagtggca ggcgcccgcg cccgcgaccc gcacgagcac
      721 ctgacccggc gccggctcgg ggacgggcac gtcgcgtagt tccggcggtt cgccccaggc
      781 ggtcaactgc agcgcgcgca tgggcgacaa ggtaggcgat ggctggggac ggcccggtcc
      841 cgccgctacg gtctgcgccg tgaccgatgt cgtcgctcgc tccgccggcc tcaccgcggc
      901 cgaggttgcc gagcgtgtcg cacggggcga cgtcaacacc gccgacgagc gcacgagccg
      961 tccgctgtcg gagatcatcc gggcgaacgt cttcacgcgc ttcaacgcga tcttgggcac
     1021 gatgctcgtg ctcatcatca tcgtcgggcc gttccaggac gcgctcttcg gtgtcgtcct
     1081 ggtcgcgaac acgctcatcg gcatcgtgca ggagtggcgc gccaagcgca ccctcgaccg
     1141 cttggcggtg ttgaacgcgc cgcaggcgcg ggtcgtgcgc gacggccagg tgacggagat
     1201 cgccgtcgac ggtgtcgtcc tcgacgacct gctcgagctg cgcaccggtg atcaagtgcc
     1261 ggccgacggt cgcgtcgtcg aagaagacgg gctcgagatc gacgagtcgt tgctgacggg
     1321 ggagtcggag cccgtcgcca aggatgccga cgacgaggtg ctctcgggga gcatcgtcgt
     1381 ggccggtcgt ggtcgctgcg aagtcacccg tgtcggcgcc gattcctacg cgcgccgcct
     1441 ggcgaccgaa gcgcggcggt tcacgttggt gcgctcggag ttgatggacg gcatcaacct
     1501 gatcctgcgg tacgtcacgt acgcgatctt cccgacggcc gcgctgctcg cgttgagtca
     1561 gttcgaagcg cacgacggct ggcgcgaggc gatggcgggc gtcgtcgcgg gtgtcgtcgc
     1621 gatggtgccc gaagggctgg tgctgctcac gagcctcgca ttcgcgatcg cggcggtgac
     1681 gctcgcgcgg cggcaggtgc tggtacagga gctgcccgcg gtcgagggcc tggcgcgcgt
     1741 cgacgtcgtg tgtctcgaca agacgggaac gctcaccgac ggcaacatcg cgttcgatcg
     1801 ggtcgaggca ttcgacgcgg acgtcgatcc ggccgcggca ctcggtgcgc tggccgccga
     1861 cgagaaccgc aacgcgaccg cgctggcgct ggccgaggcg ttcgacgcgc cgtcgggctg
     1921 gacgcgcacg gcgacggtgc cgttctcgtc ggcgcgaaag tggagcgcgg cgtgcttcga
     1981 cggcaagggc acgtggatcc tcggtgcacc ggagaacgtg tgggccgccg cggatgaccc
     2041 ggtgcggcgc cgggccgacg agctcgcgtc cggcgggcag cgcgtgctgc tgttgacccg
     2101 cagcgacgcg ccgatcaccg cggaggtact cccgcccgac atgcaggcgg tcgcgctcgt
     2161 gctcttcgag gagcagatcc gccccgacgc cgccgacacc ttggcgtact tcgcgcagca
     2221 aggtgtgtcg ctgaaggtca tctccggtga caacccgcgc acggtcggcg cggttgcgtc
     2281 gcgcgtgggg ctgcccgggg cgcacgagcc ggttgacgcg cgcgagctcc ccgaagacgg
     2341 cgacgagctc gccggggtgc tcgaggagtc gagtgtgttc gggcgagtca cgccgcacca
     2401 gaagcgagcg atcgtcggcg cgttgcagtc gcgcggccac gtggtggcaa tgaccggcga
     2461 cggcgtgaac gacgcgctcg cgctgaagga tgccgacatc gggatcgcga tgggcaacgg
     2521 cgcacccgcg acgcgcgcgg tcgcccagtt ggtgctgatg gacgggcggt tcgcgacgat
     2581 gcccggtgtc gtcgccgaag gccggcgtgt catcgcgaac atcgagcgag tcgcgaacct
     2641 cttcgtcacc aagaccgtct acgcgatgtt gctcgcgatc gcggtcgggg tggctcgctg
     2701 ggagtacccg ttcctgcccc gccacctcac ggtcgtcagc tcgctgacga tcggcatccc
     2761 ggcgttcttc cttgcactgt cgccgaacac gcagcgctac atccccggtt tcgtcgtgcg
     2821 cgtcctgaag ttcgcggtgc cgtgtgggtt cgtcgcggcc gcggcaacgt tcgggtccta
     2881 tgccgcggcg cgcgacatcg cacacgtcga cctcgtcgag cagcggacca ccgcgacgtt
     2941 gacgctgctg gtcatcggcc tgtgggtgct gggcatcctg gcccggccga tcacgccgtt
     3001 ccgcgggggg ctgctcgcgg ccatgatcgc ctgcgtcgtc ggtgtgatca cgattccggt
     3061 gttccgcgac ttctacgagt tcgacatgcc cgaaggtgct gcgctcgtgg tctgcgcggt
     3121 cgtctcggtg gtcgcctgcg cgttgatcga gctcgggtgg atcttctcgc agcaccgcaa
     3181 ggagccggac gcccgcacac cgcgcctcgc gctccgcaat cccgcggctc gtgctgctgc
     3241 tacagccagt cgcgacgttt gaagtagacg tacaaggcca cggtgacgac gccgatcagt
     3301 ccgagcgcga tcgggtagcc gtagtaccag gtgagctcgg gcatgtgctc gaagttcatg
     3361 ccgtagacgc ccgtgatgag cgtcgcgacg atgaggattg cgccccacga cgacgtgagc
     3421 ttcatcacgc ggttcgtccg gttcgacatc accgcgagct gaccttcgag gaggccggtg
     3481 agcagctctc gttgcgactc gatcaggtcg agcacgcgca gcacatggtc gagcacgtcc
     3541 tgaaggcgta cgagagcggg ctcggtgatg cacgacactt cgcgccgcag cagctcgttc
     3601 agcacctcac gcagcggtcc gacctggcgg cggaaacgta cgaggccgcg ccgcaagacg
     3661 aacagctttt gcgggatgcc ttccgcgcgc tcgttgaaga ccagctcctc gatctcgtcg
     3721 atgatctcgt cgaccgcgtc ggtgacgtcg aagtagcggt cgacgacgac gtcgaggatg
     3781 gcccacaaca cgaatccctc gtcgtctgca ccgtcttcgc cgcggcggcg ctcgaaacgg
     3841 cgcaacgcct cgtcgatcag ctccgggtcg atgccgccgg gcgcgcgacg gaccgacagg
     3901 agccagccct cgcggaacac gatgtggacc tcggtggagt gcagcccgtc atggcgcagc
     3961 acgtcgtcgt agagggcgac gtggaagtgg tcgttgaaac ggtcgagctt cgtgcgctgg
     4021 ttcccgcggg tgaggtcctc ggccgtgagg tcgtggatct tcagcgcctc gacgacgaac
     4081 gcgacgtcgt cgtcggtcgg attggtgaga tcgacccaga gcagagagtc gccgtcggcc
     4141 gcaaccgacg caatgccgtc gcgcgcaaca gtctcggtct cggtgcccga acggcgaaag
     4201 gaccacgcgc gcatcacgcg cgccgatgct acgggctcag caggtcgagg agccgggatc
     4261 cgggcgcacg accggatcga ccggcggagt cccggcactg ggcgccggac ccgcgcccat
     4321 cgttcgcccg aacgacgtcg ttgcgaacat ggagccgttc ggcgcgcagt acacgccgat
     4381 gccgacgagg tcgaagcccg gcgagagcat gttggagcgg tggccgtcgg agtgcatcca
     4441 cgcgacgtgc aggccaccgg cgctgacgcc cgcgctgccc gacgcgatgt tctcgccgac
     4501 gaggccgaag cggccgccgt tcaacaggtt gccgaggttg gagtggcgga agccgttgac
     4561 cgacatgtcg cggctccagt cggtggcgta ctgcgcgaga cccggatccc aacccaacgc
     4621 cgggagcccg cgagcggcgc gctcggcgtt gaggcggtcg aagaagtcgc gggcggccgc
     4681 gtcggatctc gacgcgccgg ccggaccaca cgtgggcgcg gcgctcgacg gtgagaaggc
     4741 gtacgtccgc gcgtcagtga atgcgatcca gtagccacgt gagccacgcg acatcgcgat
     4801 c
//
LOCUS       DASUGA010000556         2567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_22081, whole genome shotgun sequence.
ACCESSION   DASUGA010000556 DASUGA010000000
VERSION     DASUGA010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22081"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /gene="proC"
                     /locus_tag="VFX21_11030"
     CDS             <1..753
                     /gene="proC"
                     /locus_tag="VFX21_11030"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX5096542.1"
                     /translation="GGLLDGGWDADAISVAEIDVDRRRDLEQQFSKIRVVPSASWAVA
                     DADIVIVAVKPGDVPATLESAMQALADDALVLSIAAGVTIATLEQAAPGRPVVRAMPN
                     TPALVGRGASAIAPGANATDEHLELAARVLGSVGIVVKVSEAALDAVTGLSGSGPAYV
                     MLVAEAMIEAGVLNGLPRGTAEELVVQTLLGSAELLAQGDTSPEALRAAVTSPGGTTA
                     AGLRELEARGLRAAFLDAISAATERSRELGRE"
     gene            750..1535
                     /locus_tag="VFX21_11035"
     CDS             750..1535
                     /locus_tag="VFX21_11035"
                     /inference="COORDINATES: protein motif:HMM:NF027034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HEX5096543.1"
                     /translation="MKARNRPPLLESRAMVRMPPVPPTDADLLVRAKVAGPAPTIGPS
                     EQRHLVEHLGASAPEAWAAMRAVYGTSPEAPMIDPERTLGATRRAAARIHAVAAVGGR
                     IAFATAAPASLLGVYSSIVRLARAAGAVVDDDQDRGPLRVDGRYPRWLRTVDGVTVVT
                     DGSSLLATTGIDAADEWVFLVDRPALAVADGPFAAAAYADGVEVVTIAGLDQPALGLA
                     AARTLRCTLVPLHPSRPPRDYDPFVTGFAQAFTSECGRSGRAM"
     gene            1622..1816
                     /locus_tag="VFX21_11040"
     CDS             1622..1816
                     /locus_tag="VFX21_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5096544.1"
                     /translation="MPQELSKARFLTVQEVADLMRVSSMTVYRLIKAGDLPAVRVGRS
                     FRVSETDVDAYLASRYTQAG"
     gene            1955..>2567
                     /locus_tag="VFX21_11045"
     CDS             1955..>2567
                     /locus_tag="VFX21_11045"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEX5096545.1"
                     /translation="MAARDFVSFLSDYGRGDEFVGVCHAVMLDIAPHLRVVDITHDVP
                     PFDVRAGGLALVRAIQYLPEGVVLAIVDPGVGTDRRCIAVEIEHGFLVGPDNGLLAPA
                     VAMLGGPRRVVSLENEEYQLPAPGPTFAGRDVMAPAAAQLCLGLELTKLGPEVDPATL
                     SPGLVPLPELKDGTIAAEVLWVDRFGNCQLNVDPLMLAELGAAE"
BASE COUNT          325 a          905 c          936 g          401 t
ORIGIN      
        1 ggcgggttgc tcgacggtgg ctgggacgcc gacgcgatct ccgttgccga gatcgacgtc
       61 gaccgccgcc gcgatctcga gcagcagttc tcgaagatcc gcgtggtgcc gagtgcttcg
      121 tgggccgtcg ccgatgctga catcgtgatc gtcgccgtga agcccggcga tgtgcccgcg
      181 acactcgagt cggcgatgca ggcgttggcc gacgacgcac tcgtgttgtc gattgccgcg
      241 ggcgtcacga tcgcgacact cgagcaggcc gcgcccggcc ggcccgtggt gcgcgcgatg
      301 ccgaacacgc cggcgctcgt tgggcgcggt gcgtccgcga tcgcgccggg tgcgaacgcg
      361 accgacgagc atctcgagct cgccgcgcgc gtgctcgggt cggtcggcat cgtcgtgaag
      421 gtgtccgagg ccgcgctcga cgcggtcacc gggttgtcgg gttcgggacc ggcgtatgtg
      481 atgctcgtcg cggaagcgat gatcgaagcg ggcgtcctca acggactgcc ccggggcact
      541 gccgaggagt tggtggtgca gacactgctg ggttcggcgg aattgctggc ccagggcgac
      601 acctcgcccg aggcgttgcg cgcggcggtc acgtccccgg gtgggacgac cgccgccggg
      661 ttacgggagc tggaagcgcg aggacttcgc gccgcgttct tggacgcgat atccgcagcg
      721 acggaacgct ctcgtgaact cgggcgcgag tgaaggcgcg gaaccggccg ccgttgctcg
      781 agtcgcgggc catggtgcgg atgccgcccg tgccgccgac cgacgcggac ctgttggtgc
      841 gcgccaaggt cgcggggccg gcgccgacga tcggtccctc ggagcaacgt cacctcgtcg
      901 agcatctcgg agcgagtgcg cccgaagcgt gggccgcgat gcgcgcggtc tacggcacgt
      961 cgcccgaggc gccgatgatc gaccccgagc gcacgctggg ggcaacccgc cgcgccgcgg
     1021 ctcggatcca cgcggttgcg gccgtcggtg ggcgcatcgc gttcgccacc gccgcgcccg
     1081 cgtcgctgct gggtgtgtac agctcgatcg tccggctcgc acgcgccgcg ggtgcggtgg
     1141 tcgacgacga ccaggaccgc ggtccgctgc gcgtcgacgg tcggtatccg cgctggctgc
     1201 gaacggtcga cggtgtcacg gtcgtcaccg acgggtcgtc gctcctcgcg acgactggca
     1261 tcgacgccgc cgacgagtgg gtcttcctcg tcgaccgccc cgcgctcgcg gttgccgacg
     1321 gcccgttcgc ggccgccgcc tacgccgacg gggtcgaggt ggtcacgatc gccggcctcg
     1381 accagccggc cctgggtctc gccgcggccc ggaccttgcg gtgcacattg gtgccgctcc
     1441 acccatctcg cccgccccgt gactacgacc cgttcgtcac cgggttcgcg caggccttta
     1501 cgtccgaatg cggacgatct ggccgcgcaa tgtgacgtaa cacactcatc gggagacacg
     1561 ctcgacaact tcggcctcac gggcgtatgg tcgccgcagc ggagggagta gggagatccg
     1621 cgtgccccag gaactgagca aagcgcgctt cctcacagtg caggaagttg cggatctgat
     1681 gcgggtgtcg tcgatgacgg tgtatcgctt gatcaaggcc ggggatcttc ccgcggtccg
     1741 cgtcgggcgg tcgttccgag tcagcgaaac cgacgtcgac gcctacctgg cgtcgcgtta
     1801 cacccaagcc ggctgagccg agcggagccg acggctcccg gcgcgtgttc cgcgcgcgac
     1861 accagcgagg cggggcccga gcggcctggt gccgaaggcg ccgagagcgg gacttttgtc
     1921 ggctgcggcc cgggcgccgg tagcgtccgc ggcggtggcg gctcgcgact tcgtctcgtt
     1981 tctctccgac tacggccgcg gcgacgagtt cgtcggcgtg tgccatgcgg tcatgctcga
     2041 catcgcgccg catctgcgcg tcgtcgacat cacccacgac gtcccgccgt tcgacgtgcg
     2101 tgccggcggc ctcgcgctcg tgcgggcgat ccagtacctg cccgaaggcg tcgtcctcgc
     2161 gatcgtcgat cccggcgtcg gcaccgaccg gcgctgcatc gcggtcgaga tcgagcacgg
     2221 tttcctcgtc ggtcccgaca acgggctgct cgcgcccgcg gtcgcgatgc tcggcggtcc
     2281 ccggcgcgtc gtctcgttgg agaacgagga gtaccagctg cccgcgccgg gcccgacctt
     2341 cgccggccgc gacgtcatgg cgccggccgc cgcccagctg tgcctcggcc tcgagctcac
     2401 caagctcggt cccgaggtcg atcccgccac gctcagcccg ggcctggtgc cgctgcccga
     2461 gctgaaggac ggcacgatcg cggccgaggt gctctgggtc gaccgcttcg gcaactgcca
     2521 gctgaacgtg gacccgctca tgctcgccga gctcggcgcg gccgagg
//
LOCUS       DASUGA010000557         4739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1411402, whole genome shotgun sequence.
ACCESSION   DASUGA010000557 DASUGA010000000
VERSION     DASUGA010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411402"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VFX21_11050"
     CDS             <1..546
                     /locus_tag="VFX21_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5096546.1"
                     /translation="RTIDGESHCCEVFLDDARVPVANRVGEENDGWRVTNVTLRFERG
                     TAFAQHIITLRSQIRALVALAQATGAWGDIALRTHVGRLVASVEALWRMTQMGIAEAE
                     RTGMPAPAGSAVKLRYSELAQEIADRALRTLGRPALAGADARAREIAREYLWSLQFTI
                     AAGTSQIQRNLIAERILGMPR"
     gene            567..1739
                     /locus_tag="VFX21_11055"
     CDS             567..1739
                     /locus_tag="VFX21_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5096547.1"
                     /translation="MSRDFDLLDGGWYASQPYADWAWMREHAPAYWDAKNEVWALTRY
                     HDVLAVEKDAVTYSSHRAPRPHGDHLPMMISMDAPEHSNRRKLVSRGFTPRRVREHEA
                     TVRRICTDIVDRVAPKGSCDFVWDIAAPLPLLLIADMLGFPPESYDDLLRWSDDLIRA
                     TTVDPPVEVATAGLEAMLAFREFQLGVIASRRADPQDDLVTILCHAEIDGERLDDESI
                     VQESLLILIGGDETSRHVMTDGMLALLAHPHQMQILRDDPSAIEVGVEELLRWVSPIK
                     NMSRTVTREVELHGQTLHEGDQLILMYPSANRDDAVFDDPDVFDVRRDPNPHLAFGFG
                     PHFCMGASLARLELQVMFGELLRRLPDLHLAGDPLPRRESNFISGPEAMPVRFTPA"
     gene            complement(1777..3027)
                     /locus_tag="VFX21_11060"
     CDS             complement(1777..3027)
                     /locus_tag="VFX21_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013426165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5096548.1"
                     /translation="MTSTKDTAIDALRAEVRAWLEQHWDPELPVEEWWRIVADAGWTA
                     PHFTIEEGGRGLHRNAGGTVRAEFLAFGALRPPGGLGLLMAAPTILTHGTPEQIARHV
                     RPILEGRMNWCQLFSEPGAGSDLAGLTTRAERDGERYIVNGQKVWSSMAMDADYGMLL
                     ARTDFSVPKHKGISWFAFELDQPGVTIRPLREMTGDAVFNEVFFDDAEVDAADLIGGA
                     GNGWAVTQTTLFFERSGIGAGGSHAGFPVPGPKGGMLGRRAGDAAHDHEPGANMVLQF
                     ADVAALAASQGRAQDPHVRQRLAQLYTFSKLGQWNAQRAKAEASKGGSGIANLGKIAQ
                     TLIVKHSAQLSADILGANALLAGSSAMEDGRYTDCLVFSPASSIYGGTDEIQRNIIAE
                     RTLGLPREDLPGKGEPYGKFLGRL"
     gene            complement(3024..3902)
                     /locus_tag="VFX21_11065"
     CDS             complement(3024..3902)
                     /locus_tag="VFX21_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5096549.1"
                     /translation="MQPQRDPRVVLERDDASGIARITLNNPERRNTYDPEMRAQLGAD
                     LDELALDDRVKVVLLRGEGGVFTTGADMGNAYSWYESKENGEGTARRRPSQRRRLSVD
                     RRTFAFYHDLIGYPKVTVAEVRGYALGGGLELALMADIAVVARDTVIGTPATRFLGPA
                     LGSLHLFFYRLGPVLARRLLLTGDTLPASTVEHLGVFTEVVDDVDVAARAEWYAQKVA
                     KMPADGIVMAKEAFRLVEQLQAYQGEEVASYLFHAFGTNLQFEDDEFNFVKMRAQHGT
                     RRAFELRDEHFDVPEP"
     gene            3969..>4739
                     /locus_tag="VFX21_11070"
     CDS             3969..>4739
                     /locus_tag="VFX21_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019927233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5096550.1"
                     /translation="MSFQTIRYEVEDRVATITFDRPDRLNALSPEMIRELRGAYANAE
                     ADDEVWITIVTGNGRAFCTGADVTEIPGDGRVVYDEPYLSTYAQWEAPQEGTPPFRTM
                     TKPVLAAVNGLCCGAGLDWVTTGDIVIASDRAEFFDPHVSIGLVSGREMVRLARVLPT
                     NIAMRLALTGKHERISAQRAYELGLISEVVPHDQLLDRAREIAMLVNRNAPLAVRGTR
                     LAIRKGLDVPLHEAEIMAEAFRERVVRTDDAKEGPQAFM"
BASE COUNT          747 a         1633 c         1601 g          758 t
ORIGIN      
        1 cgaacgatcg acggcgagag tcactgctgc gaggtgttcc tcgacgacgc gcgggtaccg
       61 gtagcgaacc gggtcggcga ggagaacgat ggctggcggg tgacgaacgt gacgctgcgc
      121 ttcgagcgcg ggactgcgtt cgcgcagcac atcatcacgc tgcggtccca gatccgggcg
      181 ctcgtcgcgc tcgcccaggc cacgggcgcc tggggcgaca tcgcactgcg cacacacgtc
      241 ggccggctcg tcgcgagcgt ggaagcgttg tggcgcatga cgcagatggg catcgccgaa
      301 gccgagcgca cgggtatgcc ggcgcccgcg ggttcggcgg tgaagctgcg ctacagcgag
      361 ctcgcgcagg agatcgccga ccgggcgctg cggacgctcg ggcgcccggc gctcgcgggc
      421 gcggatgcac gggcgcgcga gatcgcgcgc gaatacctct ggtcgctcca gttcacgatc
      481 gcggcgggca catcgcagat ccagcgcaac ttgattgccg agcgcatcct gggcatgcct
      541 cgctgagttt gtggaagggt gacgacatga gtcgcgactt cgacctgctc gacggcggtt
      601 ggtacgcgag ccagccgtac gcggactggg catggatgcg tgagcacgcg cccgcgtact
      661 gggacgccaa gaacgaagtg tgggcgctca cgcgctacca cgacgtgctc gcggtcgaga
      721 aggacgcagt gacctactcg agccaccgtg cgccgcgtcc gcacggcgac cacttgccga
      781 tgatgatctc gatggacgcg cccgagcact cgaaccggcg caagctcgtg agccgcggct
      841 tcacaccccg acgcgtgcgt gagcacgagg cgaccgtgcg ccgcatctgc accgacatcg
      901 tcgaccgtgt cgcgccgaaa ggatcgtgcg acttcgtctg ggacatcgcg gcgccgctcc
      961 cactgttgct catcgccgac atgctcggct tcccgcccga gtcgtatgac gacctgctgc
     1021 gctggtcgga cgacctcatc cgcgccacga cggtcgaccc tcccgtcgag gtcgcgaccg
     1081 ccggcctcga agcgatgctg gcgttccgcg agttccaact cggtgtcatc gcgtctcgcc
     1141 gcgccgaccc gcaggacgat ctcgtcacga tcctgtgtca cgcggagatc gacggcgaac
     1201 gcctcgacga cgaatcgatc gtgcaggaaa gcctgctcat cctgatcggc ggcgacgaga
     1261 cgagccgcca cgtgatgacc gacgggatgc tcgccttgct cgcgcatccc caccagatgc
     1321 agatcctgcg cgacgacccg agtgcgatcg aggtcggcgt cgaggagctc ctccgctggg
     1381 tctcaccgat caagaacatg tcgcgcaccg tcacgcgcga ggtcgagctg cacggccaga
     1441 cgttgcacga aggcgaccag ctcatcttga tgtacccgtc cgcgaaccgg gacgatgcgg
     1501 tgttcgacga ccccgacgtc ttcgacgtgc gtcgcgaccc gaacccgcat ctcgcgttcg
     1561 gcttcggccc acacttctgc atgggcgcgt cgctcgcgcg cctggagctc caagtgatgt
     1621 tcggcgagct gttgcggcgc ctgcccgacc tgcacttggc gggcgacccg ctcccgcgcc
     1681 gcgagtcgaa cttcatcagc ggccccgaag caatgcccgt ccgcttcacc cccgcgtaac
     1741 gacggtcggg cgtcttacaa gggcggaatt cgcccctcag agacgcccga ggaatttgcc
     1801 gtagggctcg cctttgccgg ggaggtcttc gcgggggagt cccagcgttc gctcggcgat
     1861 gatgttgcgt tggatctcgt cggtgccgcc gtagatcgac gacgcgggtg agaacacgag
     1921 gcagtcggtg tagcgaccgt cttccatcgc gctgctgccc gccagcaagg cgttcgcacc
     1981 gaggatgtcc gcgctgagtt gggcggagtg cttgacgatc aacgtctgcg cgatcttgcc
     2041 caggttcgcg atcccgctgc cgcccttgct cgcttcggcc ttcgctcgtt gcgcgttcca
     2101 ttgcccgagc ttcgagaagg tgtagagctg cgcgagccgt tggcggacgt gcgggtcctg
     2161 cgcgcggcct tgcgatgcag cgagcgcggc gacgtcggca aactgcagga ccatgttggc
     2221 gccgggttcg tgatcgtgcg cggcgtcacc cgcgcgacgg ccgagcatgc cgcccttcgg
     2281 cccgggcacg gggaagccgg cgtgcgagcc gcccgcgccg atgccggacc gctcgaagaa
     2341 caacgtcgtc tgcgtgacgg cccagccgtt gccggccccg ccgatgaggt cggcggcgtc
     2401 gacctcggcg tcgtcgaaga acacttcgtt gaacaccgcg tcgccggtca tctcgcgcaa
     2461 cggccggatg gtgacgccgg gctggtcgag ctcgaacgcg aaccacgaga tgcccttgtg
     2521 cttcggcacg gagaagtcgg tgcgggcgag cagcatcccg tagtcggcgt ccatcgccat
     2581 gctgctccac accttctgac cgttgacgat gtagcgctca ccgtcgcgct cggcgcgcgt
     2641 cgtgaggccc gcgaggtcgg agcccgcgcc cggttcgctg aacagctggc accagttcat
     2701 gcgaccttcg aggatcgggc gcacgtgccg cgcaatctgc tcaggggtgc cgtgcgtgag
     2761 gatcgtcggc gccgccatca agaggccgag tccaccgggc gggcgcaacg cgccgaacgc
     2821 gaggaactcg gcgcgcaccg tcccgcccgc attgcggtgc aagcctcgtc cgccttcctc
     2881 gatcgtgaag tgcggcgcgg tccagcccgc gtcggccacg atgcgccacc actcctcgac
     2941 cgggagttcc gggtcccagt gctgctcgag ccacgcgcgg acctcggcgc gcagagcgtc
     3001 gatcgccgtg tccttggtgc tcgtcacggc tcgggcacgt cgaagtgctc gtcgcgcagc
     3061 tcgaacgcgc gcctcgtccc gtgttgggcc ctcatcttga cgaagttgaa ctcgtcgtcc
     3121 tcgaactgca agttcgtgcc gaacgcgtgg aagagatagc tcgccacttc ttcgccttgg
     3181 tacgcctgga gctgctcgac caggcggaag gcctccttgg ccatgacgat gccgtcggcg
     3241 ggcatcttgg cgaccttctg cgcgtaccac tccgctcgtg ccgcgacatc gacatcgtcg
     3301 acgacctcgg tgaacacacc gagatgctcg acggtcgacg ccggcagcgt gtcgccggtc
     3361 agcaacaacc ggcgcgcgag taccggtccg agccggtaga agaagaggtg cagtgagccg
     3421 agcgcggggc cgaggaaccg cgtcgccgga gtgccgatca ccgtgtcgcg cgcgacgacg
     3481 gcgatgtcgg ccatcaacgc gagctcgaga ccgccaccca acgcgtagcc gcgcacttcc
     3541 gcgacggtga ctttcggata gccgatgagg tcgtggtaga acgcgaacgt ccgccggtcg
     3601 accgacagcc ggcgccgctg actcgggcgc cggcgcgccg tgccctcacc gttctccttc
     3661 gactcgtacc agctgtacgc gttgcccatg tcggcgccgg tcgtgaacac gccgccttcg
     3721 ccgcgcagca gcacgacctt gacgcgatcg tcgagcgcga gctcgtcgag atcggcgccc
     3781 aactgcgcgc gcatctcggg gtcgtacgtg ttgcgccgct ccgggttgtt gagcgtgatg
     3841 cgggcgatcc cgctcgcgtc gtcgcgctcg agcacgacgc gcggatcacg ttgcggctgc
     3901 acggcgccaa ccctacactc ttatatctag atatttctac ctcgtgagga tgcgagaggc
     3961 tacggaacat gagcttccag acgatccgtt acgaggtcga ggaccgcgtc gcgaccatca
     4021 ccttcgaccg tcccgaccgg ctcaacgcgc tcagtcccga gatgatccgc gagctgcgcg
     4081 gcgcgtacgc gaacgcggag gccgacgacg aggtctggat cacgatcgtc accgggaacg
     4141 gccgtgcctt ctgcaccggt gccgacgtca ccgagatccc cggcgacggt cgcgtggtgt
     4201 acgacgagcc gtatctctcg acgtacgcgc agtgggaagc accgcaggaa ggcacgccgc
     4261 cgttccgaac gatgaccaag ccggtgctcg cggccgtcaa cgggctgtgc tgcggtgccg
     4321 gtctcgactg ggtgacgacc ggcgacatcg tgatcgcgtc cgaccgcgcc gagttcttcg
     4381 acccccacgt cagcatcggc ctcgtctcgg gccgcgagat ggtgcggctg gcgcgtgtcc
     4441 tgcccacgaa catcgcgatg cggctcgcgc tcaccgggaa gcacgaacgg atcagcgccc
     4501 agcgcgcgta cgagctcgga ctgatctccg aagtcgtgcc gcacgaccag ttgctcgatc
     4561 gcgcgcgcga gatcgcgatg ctcgtgaacc gcaacgcgcc gctcgccgtg cgcggcactc
     4621 gtctcgcgat ccgcaaggga ctcgacgtgc cgctgcacga ggccgagatc atggccgagg
     4681 cgttccggga acgagtcgtg cggaccgacg acgcgaaaga aggcccgcag gcgttcatg
//
LOCUS       DASUGA010000558         3632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1298647, whole genome shotgun sequence.
ACCESSION   DASUGA010000558 DASUGA010000000
VERSION     DASUGA010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298647"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /gene="carA"
                     /locus_tag="VFX21_11075"
     CDS             <1..918
                     /gene="carA"
                     /locus_tag="VFX21_11075"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX5096551.1"
                     /translation="FTYPHIGNYGTNGDDDEAPVPYARGVIVRDLARRPSNWRATEDL
                     DGFLRRHGVSGIAGLDTRRLTRHIRSAGAMPGAFGVADRDTLLAAAVADGGTDGCDLA
                     SVVSTKEEYTVGEANASYYVVAYDFGIKRSILDQLVDAGCQVDVVPAQTLAADVLARE
                     PDGVFLSNGPGDPGAVTGPIDEVAGLLGNVPVFGICLGHQIMGLALGGERFKLRFGHH
                     GGNHPVRQLATGRVEITSQNHNYAVDADSLPDGSVVSHLNLNDGDVEGIRTERLQAFS
                     VQYHPEAGPGPHDARYLFREFTDLMKANA"
     gene            918..>3632
                     /gene="carB"
                     /locus_tag="VFX21_11080"
     CDS             918..>3632
                     /gene="carB"
                     /locus_tag="VFX21_11080"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX5096552.1"
                     /translation="MPRRDDLESILLIGSGPIVIGQACEFDYSGTQACRVLRDEGYRV
                     VLVNSNPATIMTDPEFADATYIEPLDVPTLTRIIERERPDALLPTLGGQTGLNLAVAL
                     HEAGVLERFGVEMIGANIEAIQTAEDRQKFKAAMIEIGLSVPPSGIAYTVDDALRIAE
                     DVGYPVIVRPAFILGGGGTGIAHDRNEMSHLATRGLAASPISEILIERSITGWKEYEL
                     EVMRDHADNVVVICSIENVDAMGVHTGESITVAPAQTLTDVEYQHMRDAAFQCIRRIG
                     VETGGSNVQFAVHPLTGDMVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYRLDE
                     VQNDITRETPASFEPSIDYVVTKFPRWAFEKLPGAAPVLGTQMQSVGEVMAIGRTFTE
                     SLQKAVRSLETGRYGLNCDPGEDHFDALTADELAQAAAVATPERIFQLEAALRKFVSV
                     ERLHEVTGIDPWFLDQLLQIVEERDRLQQLGFAAMRRADWRQAKRFGFGDAQLAYLWG
                     VAADDVRAARIAAGVAITYKTVDTCAAEFDAFTPYHYGTYEDEDEVAPLTRDAVVILG
                     SGPNRIGQGVEFDYCCVHAAFALHDARYETVMVNCNPETVSTDYDTSDRLFFEPLSEE
                     GVRNVCDALFAAAERGGGSLAGVIVSLGGQTPLKLAHALERAGVPILGTTPASIDLAE
                     DREAFNALCERLGIPQPPGGTATDAEGAVAIAASIGYPVLVRPSYVLGGRAMEIVYDD
                     SDLRRAMGELTQSGSLGREGGLSAERPALVDRFLEDAIEVDVDAIRDRTGEVVLGGIM
                     EHIEEAGVHSGDSACAIPPPTLPSWVVEVIEAYTAAIAERLDVRGLINVQFAVKSGQV
                     FVIEANPRASRTVPFVAKATGVPLANAAARIMLGATIAGLRAEGVLPAH"
BASE COUNT          573 a         1235 c         1262 g          562 t
ORIGIN      
        1 ttcacatacc cgcacatcgg caactacggc acgaacggcg acgacgacga ggcaccggtg
       61 ccgtacgcgc gcggcgtcat cgttcgtgac cttgcgcgcc ggccgtcgaa ctggcgcgcc
      121 accgaagacc tcgacggttt cctgcgccgg cacggggtgt cgggcatcgc gggtctcgac
      181 acgcgccgcc tgacgcgcca catccgcagc gcgggcgcga tgccgggcgc gttcggtgtc
      241 gccgaccgcg acacgttgct cgcggctgcc gtcgccgacg gcggcaccga cggttgcgat
      301 ctcgcgagcg tggtgagcac gaaagaggag tacacggtcg gcgaggcgaa cgcgtcgtac
      361 tacgtcgtcg cgtacgactt cggcatcaaa cggtcgatcc tcgaccagct cgtcgacgcc
      421 ggctgccaag tcgacgtcgt ccctgcacag acgttggcgg ccgacgtgct cgcccgcgaa
      481 cccgacggcg tgttcctctc gaacggtccc ggcgacccgg gcgcggtcac gggcccgatc
      541 gacgaggtcg cgggattgct cggcaacgtg cccgtgttcg gcatctgcct cggccaccag
      601 atcatgggcc tcgcgctcgg cggcgaacgc ttcaagctcc gcttcgggca ccacgggggc
      661 aaccatccgg tgcggcaact cgcgacgggc cgggtcgaga tcacgagcca gaaccacaac
      721 tacgcggtcg acgccgacag cctgcccgac ggctccgtcg tcagccacct gaacctgaac
      781 gacggcgacg tcgagggcat ccgcaccgag cggttgcagg cgttcagcgt gcagtaccac
      841 cccgaagcgg gtcccggacc gcacgacgcc cgttacttgt tccgcgagtt caccgacttg
      901 atgaaggcga acgcctgatg ccgcgccgcg acgatctcga atcgatcctg ctgatcggca
      961 gtggcccgat cgtcatcggg caagcgtgcg agttcgacta ctccggcacg caggcgtgcc
     1021 gggtgctgcg cgacgagggg taccgcgtcg tgctcgtgaa ctcgaatccg gcgacgatca
     1081 tgaccgaccc cgagttcgcc gacgcgacgt acatcgaacc gctcgacgtc ccgacgttga
     1141 cgcgcatcat cgagcgcgaa cggcccgacg cgttgttgcc gacgctcggc ggccagaccg
     1201 gattgaacct cgcggtcgcg ttgcacgagg cgggcgtgct cgagcgcttc ggcgtcgaga
     1261 tgatcggcgc caacatcgag gcgatccaga ccgccgaaga ccgccagaag ttcaaggccg
     1321 cgatgatcga gatcgggctg tcggtcccgc cgtcgggcat cgcgtacacg gtcgacgacg
     1381 cgctgcgcat cgcggaagac gtcggctacc ccgtgatcgt gcgccccgcg ttcatcctcg
     1441 ggggcggcgg gacgggcatc gcgcacgacc gcaacgagat gtcgcacctc gcgacccgcg
     1501 gtctcgccgc gagcccgatc tcggagatcc tcatcgagcg gtcgatcacc ggttggaagg
     1561 aatacgagct cgaggtgatg cgtgaccacg ccgacaacgt ggtcgtcatc tgttcgatcg
     1621 agaacgtcga cgcgatgggt gtgcacacgg gcgagtcgat cacggttgca cccgcgcaga
     1681 cgctcaccga cgtcgagtac cagcacatgc gcgacgccgc gttccagtgc atccgtcgta
     1741 tcggcgtcga gaccggcggt tccaacgtcc agttcgccgt ccatccgctg accggcgaca
     1801 tggtcgtcat cgagatgaac ccgcgcgtgt cgcgttcgag cgcgctggcc agcaaggcga
     1861 ccgggttccc gatcgccaag atcgcggcga agctcgcggt cgggtaccgg ctcgacgaag
     1921 tgcagaacga catcacgcgc gagacgcccg cgtcgttcga gccgtcgatc gactacgtcg
     1981 tcacgaagtt cccgcgctgg gcgttcgaga agctgcccgg tgcggcgccg gtgctcggca
     2041 cgcagatgca gtcggtgggc gaggtgatgg cgatcggccg tacgttcacc gagtcgttgc
     2101 agaaggcggt gcgctcgctc gagaccggtc ggtacggtct caactgcgac cccggcgaag
     2161 accacttcga cgcgctcacc gcggacgagc tcgcgcaggc cgcggccgtc gcgacgcccg
     2221 agcgcatctt ccagctcgaa gctgcgctgc ggaagttcgt gtcggtcgag cgcctgcacg
     2281 aggtgaccgg catcgatccg tggttcctcg accagttgct ccagatcgtc gaggaacgtg
     2341 atcggttgca acagctcggc ttcgcggcga tgaggcgcgc cgactggcgc caggcgaagc
     2401 ggttcgggtt cggcgacgcg cagctcgcgt acttgtgggg cgtcgccgcc gacgacgtcc
     2461 gtgccgcacg catcgcggcc ggtgtcgcga tcacgtacaa gaccgtcgac acgtgcgcgg
     2521 ccgagttcga cgcgttcacg ccgtaccact acggcaccta tgaagacgag gacgaagtcg
     2581 cgccgctcac gcgcgacgcg gtcgtcatcc tcgggagtgg ccccaaccgc atcggccagg
     2641 gtgtcgagtt cgactactgc tgcgtgcacg ccgcgttcgc gttgcacgac gcccgttacg
     2701 agacggtgat ggtcaactgc aacccggaga cggtgtccac cgactacgac accagcgacc
     2761 gcttgttctt cgagccgctc tccgaagagg gtgtgcgcaa cgtgtgcgac gcgctgttcg
     2821 cggcggccga acgcggcggc ggttcgctcg cgggtgtgat cgtcagtctg ggtggccaga
     2881 cgccgttgaa gcttgcgcac gcgctcgagc gcgcgggcgt cccgatcctc gggacgacgc
     2941 cggcgtcgat cgatctcgcc gaggaccgcg aggcgttcaa cgcgctctgc gagcggctcg
     3001 gcatcccgca gccgccgggt ggcaccgcga ccgacgccga gggcgcggtc gcgatcgcag
     3061 catcgatcgg gtatccggtg ttggtgcggc cgtcgtacgt gctcggcggg cgcgcgatgg
     3121 agatcgtgta cgacgacagc gacctgcggc gcgcgatggg ggagctgacg cagtcgggtt
     3181 ccctcggtcg ggagggcggt ttgtcggccg aacgccccgc gctcgtcgac cgcttcctgg
     3241 aggacgcgat cgaagtcgac gtcgacgcga ttcgcgaccg caccggtgag gttgtgctcg
     3301 gcgggatcat ggagcacatc gaggaagcgg gtgtgcactc cggtgattcc gcgtgcgcga
     3361 tcccgccgcc gacgctgccg tcgtgggtgg tcgaggtcat cgaggcgtac accgcggcga
     3421 tcgccgagcg cctcgacgtc cgcgggttga tcaacgtgca gttcgccgtg aagagcggtc
     3481 aggtcttcgt catcgaggcc aaccctcgcg cgtcgcgcac cgtgccgttc gtcgccaagg
     3541 ccaccggcgt gccgctcgcc aacgcggccg cgcggatcat gttgggcgcg accattgccg
     3601 ggctgcgtgc cgagggcgtg ctgcccgcgc ac
//
LOCUS       DASUGA010000559         3308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_578346, whole genome shotgun sequence.
ACCESSION   DASUGA010000559 DASUGA010000000
VERSION     DASUGA010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578346"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VFX21_11085"
     CDS             complement(<1..237)
                     /locus_tag="VFX21_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096553.1"
                     /translation="MLDLDDGGDYVVLGDCAAVLPKLADASFQLVYVDPPFNTGKAQL
                     RRTLRTVRAGTDDASDRIGFQGRAYTTTELGRMHY"
     gene            294..932
                     /locus_tag="VFX21_11090"
     CDS             294..932
                     /locus_tag="VFX21_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5096554.1"
                     /translation="MSEHTFAFVDLAGFTALTEAHGDEEAVATLARFRTITNAALGTD
                     GVLVKTIGDAVMLAFPSPDRAMAALRRMFDAAASDANLPLIRAGAHHGSALAEGGDYF
                     GASVNLAARVAAQAGGGQLLATSAVALAARDAGEVVTHVGPTELRNIAAPVDLYEISF
                     HVSAETAVDPVCAMKVPIGDPATVHIHFGDRDFWFCGLPCVATFASAPERYL"
     gene            complement(929..1384)
                     /locus_tag="VFX21_11095"
     CDS             complement(929..1384)
                     /locus_tag="VFX21_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5096555.1"
                     /translation="MKQGTRGEARVHIDAPPEKVYAVVSDVTRMGEWSPETKTCAWLD
                     GATGPAVGARFKGTNKRGLFKWSTKPTVVAAEPGCEFTFEVNGTRWSYRFAAADGGTD
                     AIESFEMLKDVPWLYVQAERLMGVKDRKADLEQAMQTTLERVKAAVEGT"
     gene            1459..2388
                     /locus_tag="VFX21_11100"
     CDS             1459..2388
                     /locus_tag="VFX21_11100"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HEX5096556.1"
                     /translation="MPSDALAEALAATRESRRVAFKRHGSPAEAGAWLDVVKDVVAIA
                     NSGGGVVIVGVDSHGQPNGWSGPFDAPALIDAVAHYTGDHFTDIAVEAATKGARPVTA
                     ISIGARSGSPLVFEKAGQFVDDHGRHRVSFARGTLYFRHGSRTEPARGRDVAKFATGE
                     LGREKRGIARNVSKVAQAPVGSEVIIVPPATALPGVLERFRVVDDPRAPAVARTDFDI
                     THPFRQKELIERINERLGEKLVGPYEVLSIRRVHATDERPEFFHRPKFGSPQYSDAFV
                     SWIAEQVRHDPEFLADAKQRYQATLPKRGRRGP"
     gene            2424..2876
                     /locus_tag="VFX21_11105"
     CDS             2424..2876
                     /locus_tag="VFX21_11105"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-responsive transcriptional
                     regulator"
                     /protein_id="HEX5096557.1"
                     /translation="MRISELATQAGIAPSTLRYYERIGLMPSPGRTASGYRSYGNDDV
                     ARLLFVTRAKRIGLTLEQIAELLQVWDGVNCGTTFERITGLVAEKRTEIADRITELQQ
                     FAQQLDVVSAALDEITPPATCRPDLSCCLPETNADTAIVVDLIPRPRH"
     gene            2927..3100
                     /locus_tag="VFX21_11110"
     CDS             2927..3100
                     /locus_tag="VFX21_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096558.1"
                     /translation="MPRISKSDASEVMELEGYEGHFGRLGDYTIGFETYTADADLAEL
                     FKGLPDDRCQCPH"
     gene            3107..3298
                     /locus_tag="VFX21_11115"
     CDS             3107..3298
                     /locus_tag="VFX21_11115"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096559.1"
                     /translation="MLKGKLKFTTDEGETVIEAGDAYYVPPGHLPYLYAGAEVVEFSP
                     TTQLNETMAVIEKNTANPS"
BASE COUNT          539 a         1165 c         1092 g          512 t
ORIGIN      
        1 gtagtgcatg cgaccgagct cggtggtcgt gtacgcgcgg ccctggaaac cgatgcggtc
       61 cgacgcatcg tcggtgccgg cccggacggt gcgcaacgtg cgccgcagct gcgccttccc
      121 ggtgttgaac ggtgggtcga cgtagacgag ctggaacgat gcgtcggcca gcttcggcag
      181 gacggccgcg cagtcgccga ggacgacata atccccgccg tcgtcgaggt cgagcacgcg
      241 cggaacctag cccgcggccg actggcgggc cgcgaccggg gtaacgcaag gacgtgagtg
      301 agcacacctt cgccttcgtc gaccttgccg gcttcaccgc gctgaccgaa gcgcacggcg
      361 acgaagaggc ggtcgccacg ctcgcccggt tccgcacgat cacgaacgcg gcgttgggta
      421 ccgatggtgt gctggtcaag acgatcggcg acgcggtgat gctcgcgttc ccgtcacccg
      481 atcgcgcgat ggcggcactg cgccgaatgt tcgacgcggc ggcgtccgat gccaacctgc
      541 cgctcatccg cgccggcgcg caccacggga gcgcgctcgc ggaaggcggc gactacttcg
      601 gcgcgtcggt gaatctcgcg gcgcgcgttg ccgcgcaagc cggtggcggt cagctcctgg
      661 cgacgagcgc ggtcgcgctc gccgcgcgcg atgccggtga agtcgtgacg cacgtcgggc
      721 cgaccgaact gcgcaacatc gccgcgcccg tcgatctgta cgagatcagt ttccacgtct
      781 cggccgagac cgccgtcgac cctgtgtgcg cgatgaaggt cccgatcggc gaccccgcga
      841 ccgtgcacat ccacttcggc gatcgcgact tctggttctg cgggctcccg tgtgtcgcaa
      901 ccttcgcgag cgcgcccgag cggtacctct aagtaccttc gactgccgcc ttgacgcgtt
      961 ccagcgtcgt ctgcatggct tgttcgaggt cggccttgcg gtccttgacg cccatcagcc
     1021 gttcggcctg gacatagagc caggggacgt ctttgagcat ctcgaaagac tcgatcgcgt
     1081 cggtaccgcc gtcggccgcc gcgaagcggt acgaccagcg cgtgccgttc acctcgaacg
     1141 tgaactcgca gcccggctcc gcggcaacga cagtcggctt cgtcgaccac ttgaacagtc
     1201 cgcgcttgtt cgtgcccttg aagcgggcgc cgaccgcagg cccggtcgcg ccgtcgagcc
     1261 aggcacaggt cttcgtctcg gggctccact cgcccatgcg ggtgacgtca ctcacgacgg
     1321 cgtagacctt ctccggcggc gcatcgatgt ggacgcgcgc ttctccacgg gtgccctgct
     1381 tcacggcgca gatccttgcg gtcgaacggc cgtcccgcaa ccgcggcacc cgtgcccgca
     1441 acggaataac gttttcgggt gcccagcgac gctctcgccg aggccctggc ggccactcgc
     1501 gagtcccggc gcgtcgcgtt caaacggcac ggctcgcccg cggaagcagg cgcgtggctc
     1561 gacgtcgtca aggacgtcgt cgcgatcgcg aactcgggcg gcggcgtggt gatcgtcggc
     1621 gtcgacagcc acgggcagcc gaacggttgg tcgggcccgt tcgacgcgcc cgcattgatc
     1681 gacgcggtcg cgcactatac gggcgatcac ttcaccgaca ttgcggtcga ggccgcgacg
     1741 aagggcgcac ggccggtcac cgcgatctcg atcggcgcgc gcagcggatc gccactcgtg
     1801 ttcgagaagg cgggccagtt cgtcgacgac cacggccggc accgagtgtc gttcgcgcgc
     1861 gggacgctgt atttccgcca cggttcgcgc accgaaccgg cgcgcggacg cgacgtcgcg
     1921 aagttcgcga cgggtgagct cggacgcgag aaacgcggca tcgcgcgcaa cgtcagcaag
     1981 gtcgcacagg cgccggtcgg gtcggaggtc atcatcgtgc ctcccgccac cgcgttgccc
     2041 ggcgtactcg aacggttccg cgtcgtcgac gacccgcgcg cgccggccgt cgcgcgcacc
     2101 gacttcgaca tcacgcaccc gttccgtcag aaggagttga tcgaacggat caacgaacgc
     2161 ctcggcgaga agctggtcgg tccgtacgaa gtcctgtcga tccgccgcgt gcacgccacc
     2221 gacgaacggc cggagttctt ccaccgcccg aagttcggtt caccgcagta cagcgacgcg
     2281 ttcgtctcgt ggatcgccga gcaagtacgt cacgatccgg agttcctcgc cgacgcgaaa
     2341 cagcgctacc aggcgaccct gcccaagcgc ggccggcgag ggccttgacc ttgtagccgg
     2401 gtgcaaggtt tacgcttttc gccatgcgga tctcggagct ggccacgcag gcggggatcg
     2461 ccccgtcgac gctgcgctac tacgagcgga tcgggctcat gccgagcccg ggccgcacgg
     2521 cgtccggcta tcgctcctac ggcaacgacg acgtggcccg gttgctgttc gtcacccggg
     2581 ccaagcgcat cgggctgacg ctggagcaga tcgccgagct cctgcaggtt tgggacggcg
     2641 tgaactgcgg caccacgttc gagcgcatca ccggcctcgt cgccgagaag cgcaccgaga
     2701 tcgcggaccg catcaccgag ctccaacagt tcgcgcagca gctcgacgtt gtcagcgccg
     2761 cgctcgacga gatcacgcca ccggcgacat gtcgccccga cttgtcgtgc tgcctccccg
     2821 agacgaacgc cgacaccgcg atcgtcgtgg atctcatccc gagaccgcgc cactgacccg
     2881 acgcgcgcct cacaacttca ctgtgcgacc aacggaaggt ttcgtcatgc ccaggatcag
     2941 caagtccgac gcgtcggagg tcatggagct cgaggggtac gaaggccact tcggccgcct
     3001 cggcgactac acgatcggct tcgagacgta caccgccgac gccgacctcg ccgagctctt
     3061 caagggactg cccgacgacc gctgccagtg cccgcactga ggcgtcgtgc tcaagggcaa
     3121 gctgaagttc acgaccgacg aaggtgaaac ggtcatcgaa gccggcgacg cgtactacgt
     3181 cccgcccgga cacctgccgt acctgtacgc gggcgccgaa gtcgtcgagt tcagcccgac
     3241 gacacagctc aacgagacga tggccgtcat tgagaagaac acggccaacc cgagctgact
     3301 gcattcgt
//
LOCUS       DASUGA010000560         2774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_300344, whole genome shotgun sequence.
ACCESSION   DASUGA010000560 DASUGA010000000
VERSION     DASUGA010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300344"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1192
                     /locus_tag="VFX21_11120"
                     /pseudo
     CDS             1..1192
                     /locus_tag="VFX21_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005438698.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
     gene            1248..2117
                     /locus_tag="VFX21_11125"
     CDS             1248..2117
                     /locus_tag="VFX21_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX5096560.1"
                     /translation="MARVVDSALAVEGGGFVVRRPFVQLDTEVTDPFLLLDEMGPVVY
                     KPGEAIGAPDHPHRGFETVTYMLDGGFQHRDSTGGGGVIRAGDTQWMTAGAGLVHSEL
                     PIEEIMRDGGRVHGLQLWVNLPRTDKLTPPQYQLITADEVTEFDTDDGRAHVKLIAGD
                     IGDHHGPGATHTPITYAHATLQPRAQLALPWPREFNALVYVLDGNGVVGPDRTPIAAG
                     QLAVYGDGDSITVAAGEDEPLVVVLLGGRPIREPIAWYGPFVMNTKAEIIQAIDDFES
                     GALGSIPPLASTS"
     gene            2114..2488
                     /locus_tag="VFX21_11130"
     CDS             2114..2488
                     /locus_tag="VFX21_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019073862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEX5096561.1"
                     /translation="MTQPLDLITEFCAAWEKGDYDALLEYFTDDAVYHNVPVDPVVGK
                     DAIRATMQMFTTGVERIEFQVNNISANGNVVLTERVDSFVLPDKTITLPVMGTFEVVD
                     GKIAAWRDYFDLNQYMQQVAGS"
     gene            complement(2454..>2774)
                     /locus_tag="VFX21_11135"
     CDS             complement(2454..>2774)
                     /locus_tag="VFX21_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase type 11"
                     /protein_id="HEX5096562.1"
                     /translation="SAEGGPCAFSLSVPARLHGLLGRAGFEDVHLTRIDQPLWVASDV
                     SDAVAFFERSAGDLRAHVPDEIVNRILLTLRASLATYVEPDGVYLPAAAWVVTSRRPA
                     ACTD"
BASE COUNT          472 a          991 c          938 g          373 t
ORIGIN      
        1 gtccggaggc gacggctgcg atcgaacgga tgatggatcg ctacgccggg gaaatcggta
       61 tggatcccgc cgacgtccgg cgcgccaaca tgctcgccga cgacgtcttc ccgtacacga
      121 cgccgacgaa ggcgacctac gacagcggcg cgtacgcggc cgcgctcgat ctcgcgctgg
      181 accgcgcggg ttacgagcgg ctgcgggccg agcgggcgca acgccgcgct cgcaacgacc
      241 ggcgccagct cggcatcggc atctcggcct acgtcgaggt caccaacgcg ggcggcgaac
      301 cggagtacgg cagcgtcgaa gtgctcgccg acggccgagc catcgcgcgc accggtacgt
      361 ccgcacacgg ccaaggccac gacaccgcgt tcgccatgct ggtgagcgcc gcgaccggca
      421 tcccgttcgg gcagatcgag gtgcggcacg gcgacaccga cgacgtgccg cggggcaacg
      481 ggaccggtgg ctcccgctcg ctccaggcag gtgggagtgc gatccgcggc gccgccgacg
      541 aactcgtcga gcgcgccaaa gcgcgcgccg cggacctgtt ggcgcgaatc ccgacgacat
      601 cgtcctcgac ctcggccgcg gccagttcca cgtcgcgggc acaccggcga tcgcgcggac
      661 gtgggccgag ctcgcgcagg ccgaagcgct cgccgccgag ttcgatttcg atcccggcgc
      721 cgcgacattc cccttcggcg cgcacattgc ggtcgtcgag gtggacgtcg agaccggcga
      781 ggcgcgtctc atacggcaca ttgcgtgcga cgacgcgggc acgatcctca atccgctgct
      841 cgtcgaaggg caagtgcacg ggggagtggc gcagggcgcg gcccaagcgc tgttcgaagg
      901 tgttgtctac gacgacgacg gcaacccgca gaccgcgaac ttcctcgact acgcgtttcc
      961 gtccgcggcc gagctgccga gcttcgaacg tgtcccgatg gagacgccca cgccgctcaa
     1021 cccgctcggc gcgaaaggga tcggcgagtc gggcacgatc ggttcgacac cggcggtgca
     1081 gaacgcggtc attgacgcgc ttgcgcacct cggcgtgacg cacgtcgaca tgccgtgcac
     1141 gcccgaacgg gtgtggaacg cgatccgagc gcgtcggcgc gtgacggaat aaccatgccc
     1201 caccaccagt tacggcgggc atgacgacct ctccacagtc ccggccggtg gcgcgcgtgg
     1261 tcgactcggc cctggccgtc gagggcggcg gtttcgtcgt ccggcggccg ttcgtccagc
     1321 tcgacaccga ggtgaccgac ccgttcctgc tgctcgacga gatgggtccc gtcgtctaca
     1381 agcccggcga ggcgatcggc gcgcccgacc acccgcaccg cggcttcgag acggtcacgt
     1441 acatgctcga cggcggcttc cagcaccgcg actcgaccgg cggcggcggc gtgatccgcg
     1501 ccggcgacac gcagtggatg accgcgggcg ccgggctggt gcactcggag cttccgatcg
     1561 aggagatcat gcgcgacggc ggacgcgtgc acggcctcca gctgtgggtc aacctcccgc
     1621 gcaccgacaa gctcacgcca ccgcagtacc agctgatcac ggccgacgaa gttaccgagt
     1681 tcgacacgga cgacggacgt gcgcacgtca agctcatcgc gggcgacatc ggcgaccacc
     1741 acggtcccgg cgcgacgcac acgccgatca cgtacgcgca cgcgaccctg caaccccgcg
     1801 cgcagctcgc actgccgtgg ccgcgcgagt tcaacgcgct cgtctacgtg ctcgacggca
     1861 acggcgtcgt cggccccgac cgcacaccga tcgcggccgg ccagctcgcg gtgtacggcg
     1921 acggcgactc gatcaccgtc gcggcgggcg aggacgagcc gctcgtggtc gtgctcctcg
     1981 gcggccgccc gatccgcgag ccgatcgcct ggtacggccc gttcgtcatg aacaccaagg
     2041 ccgagatcat ccaggccatc gatgacttcg agtcaggtgc gctcgggagc atcccgccac
     2101 tagcgtcgac ctcatgacgc aaccgctcga cctcatcacc gagttctgtg ccgcctggga
     2161 gaagggcgac tacgacgcgc tgctcgagta cttcaccgac gacgccgtgt accacaacgt
     2221 cccggtcgat ccggtggtgg gcaaggacgc gatccgggcg acgatgcaga tgttcacgac
     2281 gggcgttgag cgcatcgagt tccaggtgaa caacatctcg gccaacggca acgtcgtgct
     2341 gaccgagcgc gtcgacagct tcgtgctccc cgacaagacg atcacgctgc cggtcatggg
     2401 caccttcgaa gtcgtcgacg gcaagatcgc ggcatggcgc gactacttcg acctcaatca
     2461 gtacatgcag caggtcgccg gctcgtgacg acccacgcgg cggcgggcag gtagacgccg
     2521 tcgggttcga cgtacgtcgc gagcgacgcg cgcagcgtca gcaagatccg gttgacgatc
     2581 tcgtcgggca cgtgcgcgcg cagatcaccg gctgatcgtt cgaagaacgc gaccgcgtcg
     2641 ctcacatcgg acgcgaccca cagcggctga tcgatgcgcg tcaagtgcac gtcctcgaat
     2701 ccggcgcgcc cgagcaagcc gtgcagacgc gcgggaaccg acagcgagaa cgcgcacgga
     2761 ccgccttccg ccga
//
LOCUS       DASUGA010000561         2317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_857097, whole genome shotgun sequence.
ACCESSION   DASUGA010000561 DASUGA010000000
VERSION     DASUGA010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857097"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VFX21_11140"
     CDS             complement(<1..244)
                     /locus_tag="VFX21_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5096563.1"
                     /translation="MSVPVELTELRDAIAKQAGFAYVMTVSDDQRAHAVALVPELRGN
                     VLVCEGGNTTCRNATARSAISLVWPPRTEGDFSLIVD"
     gene            complement(241..1662)
                     /locus_tag="VFX21_11145"
     CDS             complement(241..1662)
                     /locus_tag="VFX21_11145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5096564.1"
                     /translation="MAVRMSSADGRWIIAATVLGSGIAFLDGTVVNVALRAIAEDFDT
                     NTHALQWTIDAYLVTLTALLLLGGSVGDLFGRKRVYIGGLVAFTAASVVCAVAPSSVW
                     LSAARAAQGVGGAFLVPGSLAIIAASFDEDRSRAIGAWSGLTGVASAIGPFLGGWLVD
                     AVSWRLVFLINVPLAAACVWVTVRHVPESRSHVTARPDWVGAALASLGLAALCWGLIE
                     QSLPVGLVGAALFVAFLVVESRLPAPMLPLGLFKRRQFTGANLTTLAVYSALGGAFFF
                     LVLELQIALGYSALEAGSALLPVTVLMLLLSSRAGALAQRIGPRIPMTVGPIVVGLGM
                     LLFTRIEPGMSYWRAVFPGAIVFGLGLACTVAPLTATVLAAVDADDLGIGSGVNNAVA
                     RLAGLLAVAVLPSLVAFDTDASAQAVSDAVSDAMMISAVVAVAGGVIAFLTVRTSALV
                     DEPAAMPNVVHPCGDPCRVKLSA"
     gene            complement(1690..>2317)
                     /locus_tag="VFX21_11150"
     CDS             complement(1690..>2317)
                     /locus_tag="VFX21_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096565.1"
                     /translation="ARLNELRNERTAMAQQLDAAVAERDGAVADVARYENELRRARTT
                     AEGALEEREMLRAGYTDLQQKHAEVLARVAELERALSDEQTRGREADEELRRLREAAS
                     SDARAVAAGRAEAHVEVVAEPVVEVAPIVEEEPDADDTPVVEDAEPIAKAEPDADADA
                     DADCEPEPDAESEPEGVAVDPLGRSVAMAELNAIAAFTEGEDFAPRRR"
BASE COUNT          385 a          853 c          781 g          298 t
ORIGIN      
        1 cgtcgacgat caggctgaag tcgccttcgg tgcggggcgg ccacaccagc gagatcgccg
       61 agcgggcggt ggcgttgcgg cacgtcgtgt tgccgccttc gcacaccaac acgttgcccc
      121 gcagctccgg taccaacgcg accgcgtggg cacgctggtc gtcggagacg gtcatcacgt
      181 acgcgaagcc ggcctgcttc gcgatcgcgt cacgcagctc ggtcagctcg acgggaacgc
      241 tcacgccgac agtttgacac ggcacgggtc gccgcaggga tgcacgacgt tcggcatcgc
      301 ggccggttcg tccaccagcg ccgaggtgcg gacggtgagg aacgcgatca cgccgccggc
      361 gacggcaacg accgccgaga tcatcatcgc gtcggacacc gcatcggaga cggcttgtgc
      421 cgatgcgtcc gtgtcgaacg cgacgagcga cggcaacacc gcgaccgcga gcaaccccgc
      481 gagccgggcc acggcgttgt tcacgcccga cccgataccg aggtcgtcgg cgtcgaccgc
      541 ggccaacacc gtcgcggtca gcggggcgac cgtgcacgcg agcccgagcc cgaacacgat
      601 ggcgccgggg aacaccgccc tccagtacga catgccgggc tcgatgcgcg tgaacaacag
      661 catgccgagc cccaccacga tcggcccgac ggtcatcggg atgcgcgggc cgatgcgctg
      721 cgcgagcgcg cctgcgcgcg acgacaggag gagcatgagc acggtgaccg ggagcagtgc
      781 cgagcccgct tcgagggccg agtaaccgag cgcgatctgg agctcgagta ccaggaagaa
      841 gaacgcaccg cccagcgccg agtacacggc cagcgtcgtc aggttggcac ccgtgaactg
      901 ccgccgtttg aacagcccga ggggcagcat cggtgccggc agccgcgact cgacaacgag
      961 gaacgcgacg aacagcgcgg ccccgacgag tcccaccggc aacgactgct cgatcaagcc
     1021 ccagcacagt gcggccagac cgagcgacgc gagcgcggcg ccgacccaat ccggacgggc
     1081 ggtcacgtgc gagcgtgact cagggacgtg gcgcacggtc acccacacgc acgcggcggc
     1141 gagcggcacg ttgatgagga acacgaggcg ccacgacact gcgtcgacga gccaaccgcc
     1201 gaggaacggc ccaatcgcgg aagccacgcc cgtgagcccg gaccacgcac cgatcgcccg
     1261 gctgcggtcc tcgtcgaagg atgccgcaat gatcgcgagg ctgcccggca cgaggaatgc
     1321 cccaccgacg ccctgcgcgg cacgggccgc cgagagccac accgacgacg gagcaacggc
     1381 gcacaccacc gacgcggcgg tgaacgcgac gaggccgccg atgtacacgc gcttgcggcc
     1441 gaagaggtct cccaccgatc cgccgagcaa cagcagtgcc gtgagcgtga cgaggtacgc
     1501 gtcgatggtc cactgcaacg cgtgcgtgtt cgtgtcgaag tcttccgcaa tcgcccgcag
     1561 cgcgacgttg acgacggtgc cgtcgaggaa cgcgataccc gacccgagca cggtcgcggc
     1621 gatgatccac cgaccgtccg cagagctcat ccgcacggcc atccgcgcag gctattgcgc
     1681 ccggacgggc tagcggcgac gcggtgcgaa gtcctcgcct tcggtgaatg ccgcgatcgc
     1741 gttgagctcg gccatcgcca ccgaccttcc cagcggatcc accgcaaccc cctccggttc
     1801 cgattcggcg tcgggttccg gttcgcagtc tgcgtctgcg tccgcgtccg catccggttc
     1861 ggccttcgcg atcggctccg catcctcgac aacgggcgtg tcgtccgcgt cgggctcctc
     1921 ctccacgatc ggcgcgactt ccacgacggg ctcggcgacg acctcgacgt gcgcttcggc
     1981 gcgccctgcc gcgacggcgc gcgcgtcgct cgatgcggcc tcgcggagcc gccgcagctc
     2041 ctcgtccgct tcgcgtccac gcgtctgctc gtccgacagg gcgcgctcga gctcggccac
     2101 gcgcgccagc acctccgcgt gcttctgctg gagatccgtg tagcccgcgc gcagcatctc
     2161 gcgttcctcg agcgccccct ccgcggtggt gcgagcccgc cggagctcgt tctcgtagcg
     2221 agcgacgtcg gcgacggcac cgtcgcgttc ggcgacagct gcgtcgagtt gctgcgccat
     2281 tgccgtgcgt tcgttacgca actcgttcag ccgcgct
//
LOCUS       DASUGA010000562         3283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_197548, whole genome shotgun sequence.
ACCESSION   DASUGA010000562 DASUGA010000000
VERSION     DASUGA010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197548"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..1518
                     /locus_tag="VFX21_11155"
     CDS             61..1518
                     /locus_tag="VFX21_11155"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5096566.1"
                     /translation="MPTRPNIVVLMADQHRADTIGCAGDDAVHTPHLDALAANGVRFS
                     RVSCQGPLCMPARASFLTERYVRDHGVYTNWAEVAPGTPTYLHALQAAGYYTAMLGKA
                     HLTRDDNVTVANVRELAGRMYERGFTEVVETGDKFTAQVMNPYTEHLERRGLLERYRR
                     HLADRSYHGENESGRGATKRVPMWDATPSPVPLADYIDTWHGDAAVRWIEERTAADPF
                     FLFVGFPGPHDPWDAPLEAVEKFGPPAPTMPSSTTRPDLDGLGDYGKLMRGMLQLSDT
                     DTMTDDAIAGMRRAYCANVSVIDDAVGRVVDALDRRGMLDDTWVVYTSDHGEMGGNHG
                     MMSKCVLYEQTVRVPLVVRPPGGTTGGVVDDLVEHFDIPATARGIAGAPPVPGSEARS
                     LLGYFDGDAPEARSLSISENWGFASFETDRYKLVVDEDRMRPCQLFDRHADLTEDVNL
                     VADPGARDALAELMGTVVAPFLATAPYRPHPSPFV"
     gene            1542..2402
                     /locus_tag="VFX21_11160"
     CDS             1542..2402
                     /locus_tag="VFX21_11160"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5096567.1"
                     /translation="MGERPLRQHYEGLDAQVDDELFAVYEPWRRHRDRFIAALGAMSE
                     EQASATTRCTEWDAKDILTHLVSADGFWVLTLGNSRTGAAPTKFLQGFDPSSSLDPII
                     DAQRDVSFPDQLTAFKASTDALAAEVAQWDGEDWDMHAESPFGHLAARFIFAHAFWDS
                     WLHERDTLVPLGLAGPVETDELLVAAWFTLFVAGAQGGLLGDNSPVGAGLGRPVDVTI
                     SFDDIPGRALRVRYDEAVRIEVVDPAHAKPAGRALDLVEGTAGRAPTRFDTIPDDELA
                     AQIARAAMIL"
     gene            complement(2411..>3283)
                     /locus_tag="VFX21_11165"
     CDS             complement(2411..>3283)
                     /locus_tag="VFX21_11165"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEX5096568.1"
                     /translation="DDRPMGSFADMLAAVPRGDYPAYAEAHVRELIARYRPSVLWNDI
                     AWPAAGKQLWPLFAHYYEQVPDGVVNDRWMPWNALMGATRLGPVQKAINKGAQRSAQR
                     DTGIVPPKPPYFDVRTPEYTTFPDVQRTPWECVRGMDNSFGHNAQSQSGDFLSRDELL
                     WGLADIVAKGGNLLLNVGPRGVDAQIPDEQQTRLGWLAEWPVDALRSTRPWVTYGDGD
                     VRYTARDDTVYLVVRHATASALLTSVRSTPTTQVAALDGTALDWGDTGAGLRVSVPAG
                     EPAVVVLRDVDARS"
BASE COUNT          504 a         1170 c         1120 g          489 t
ORIGIN      
        1 cacaccgcca gcgttcccga ccacgacacg gcccgaaccc cgcgggcgta gattccgggg
       61 atgccgaccc gcccgaacat cgtcgtcctg atggccgatc agcaccgcgc cgacacgatc
      121 gggtgcgcgg gcgacgacgc agtgcacacg ccgcatctcg acgcgttggc cgccaacggc
      181 gtgcgcttct cgcgcgtcag ttgccaggga ccgctgtgca tgccggcccg ggcgtcgttc
      241 ctgaccgagc ggtacgtgcg cgatcacggc gtgtacacca actgggcgga ggtcgcgccg
      301 ggcacgccga cgtatctcca cgccttgcag gccgccggct actacaccgc gatgttgggc
      361 aaggcccatc tgacccgcga cgacaacgtc accgtggcga acgtgcggga gctcgccggg
      421 cgaatgtacg agcgcgggtt caccgaggtc gtcgagaccg gcgacaagtt caccgcgcag
      481 gtgatgaacc cctacaccga gcacctcgaa cggcgcggcc tgctcgagcg gtatcgccgc
      541 cacctcgcgg accgcagcta ccacggtgag aacgagagcg gccgcggcgc cacgaagcgg
      601 gtgccgatgt gggacgcgac accgtcgccg gtgccgctcg ccgactacat cgacacgtgg
      661 cacggcgacg ccgcggttcg ttggatcgag gaacgtacgg cggccgatcc gttcttcttg
      721 ttcgtcgggt tcccgggacc gcacgacccg tgggacgcgc ccctcgaagc cgtcgagaag
      781 ttcggaccgc cggcgccgac gatgccgtcg tcgaccacgc gacccgacct cgacggtctc
      841 ggcgactacg gcaagttgat gcgcggcatg ctgcagctct ccgacaccga cacgatgacc
      901 gacgacgcga tcgccgggat gcgccgtgcg tactgcgcga acgtcagcgt catcgacgac
      961 gctgtcgggc gcgtcgtcga cgcgctcgat cgccgcggca tgctcgacga cacatgggtc
     1021 gtctacacga gtgaccacgg cgagatgggc ggcaaccacg ggatgatgtc gaagtgcgtg
     1081 ctgtacgagc agacggtgcg ggtgccgttg gtcgtgcgcc cgcccggcgg caccacgggc
     1141 ggcgtcgtcg acgatctcgt cgagcacttc gacattcccg caacggcgcg cggaatcgcc
     1201 ggcgcgccgc cggtccccgg cagcgaagcg cgctcgttgc tcggctactt cgacggcgat
     1261 gcgcccgagg cgcggtcact gtcgatcagc gagaactggg gcttcgcgtc gttcgagacc
     1321 gaccgataca agctcgtcgt cgacgaggac cgcatgcgcc cgtgccagtt gttcgaccgg
     1381 cacgccgacc tcaccgaaga cgtcaatctc gtcgccgatc ccggcgcccg cgacgcgctc
     1441 gcggaactga tgggcaccgt cgtggcgccg ttcctcgcga cggctccgta ccgcccgcac
     1501 ccgtcgccgt tcgtttgagc aggagtactg tcccggcaca catgggagaa cgtccgctcc
     1561 gccagcacta cgaaggtctc gacgcgcagg tcgacgacga gctgttcgcc gtgtacgagc
     1621 cgtggcgccg tcaccgcgac cggttcatcg ccgcgctcgg cgcgatgagc gaggaacagg
     1681 cgagcgcaac gacacgctgc accgagtggg acgccaaaga catcctgacg cacctcgtgt
     1741 cggccgacgg gttctgggtg ctcacgttgg gcaactcgcg cacaggcgcg gcgccgacga
     1801 agttcctcca agggttcgac ccgtcgagct cgctcgatcc gatcatcgac gcgcagcgcg
     1861 acgtctcgtt tcccgatcag ctgaccgcct tcaaggccag cacggacgca ctcgcagccg
     1921 aagtcgcgca atgggacggc gaagactggg acatgcacgc ggagtcgccg ttcgggcatc
     1981 tcgcggcgcg gttcatcttc gcccacgcgt tctgggattc gtggctccac gaacgcgaca
     2041 ccctcgtgcc gctcggtctg gccgggccgg tcgagaccga cgagctgctc gtcgcggcgt
     2101 ggttcacgct gttcgtggcc ggtgcgcagg gcgggttgct cggcgacaat tcgcccgtcg
     2161 gtgccggcct tgggcgcccg gtcgacgtca cgatctcgtt cgacgacatc ccgggtcgtg
     2221 cgttgcgcgt gcgttacgac gaagcggtcc gcatcgaagt cgtcgatccc gcgcacgcga
     2281 agccggccgg tcgcgcgctc gatctcgtcg aaggcaccgc gggtcgcgcg ccgacgcgct
     2341 tcgacacgat ccccgacgac gaactggccg cgcagatcgc gcgcgcggcc atgattctct
     2401 gagcgcgcgc tcaggagcgc gcgtcgacgt cgcgcagcac cacgaccgcg ggttcgccgg
     2461 cggggaccga gacccgtagg ccggcgccgg tgtcacccca gtcgagcgcg gtcccgtcga
     2521 gcgccgccac ctgggtcgtc ggcgtcgacc ggaccgaggt cagcaacgcg ctcgccgtgg
     2581 cgtgccgtac gaccagatac acggtgtcgt cgcgcgccgt atagcgcacg tcgccgtcgc
     2641 cgtacgtcac ccacggacgc gtcgagcgca acgcatcgac gggccactcg gccaaccagc
     2701 ccaagcgcgt ctgctgctcg tcggggatct gtgcgtcgac gccgcgcggg ccgacgttca
     2761 acaacaggtt gccgcccttg gccacgatgt cggccagtcc ccagagcaac tcgtcgcgcg
     2821 acaggaagtc gccggattgc gactgcgcgt tgtggccgaa gctgttgtcc attccgcgca
     2881 cgcattccca cggtgtgcgc tggacgtccg ggaaggtcgt gtactcgggc gtgcgcacgt
     2941 cgaagtacgg cggcttcggc ggcacgatgc ccgtgtcgcg ctgcgccgag cgctgcgcgc
     3001 ccttgttgat cgccttctgg accgggccga gtcgcgtcgc gcccatgagc gcgttccacg
     3061 gcatccagcg gtcgttgacg acaccgtcgg ggacttgctc gtagtagtgc gcgaacagcg
     3121 gccacagctg cttgccggcc gccggccacg cgatgtcgtt ccacaggacg ctcggccggt
     3181 agcgcgcgat gagctcgcgc acgtgcgcct ccgcatacgc ggggtagtcg ccgcgcggca
     3241 cggccgcgag catgtcggcg aacgacccca tcgggcgatc gtc
//
LOCUS       DASUGA010000563         2718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_578868, whole genome shotgun sequence.
ACCESSION   DASUGA010000563 DASUGA010000000
VERSION     DASUGA010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578868"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..1073
                     /locus_tag="VFX21_11170"
     CDS             24..1073
                     /locus_tag="VFX21_11170"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5096569.1"
                     /translation="MGSTLVHTAPVAPIFVVGAPRSGTTMLRYMLCAHPRIYIPPESN
                     FIPRFFGRRPTTPLDRRRAVDIVREIERYRVFFKDWHGPLPDPAELVDELPQPTPAGI
                     IDALYRRYASEFGAVRWGDKSPIYTRYVDLLSTIFPAARFVHIVRDGRDVATSMLHAY
                     STRRFFYYDIYYAARTWRHDVGAARESGSRLGAARYREVRYEDLVASPVDHLEAICAF
                     LGERYESAMAHPEDVARRSYHSRGIHAATRQPVTTERAGTWRTGLSRDDVAVFDTVAG
                     RLLSELCYEPRGGCAMTPRARVNFVRLRCKYTGVSAGRAALRGVGLFHPTELLARHLR
                     LRPYTRPRACSGAAG"
     gene            1070..2650
                     /locus_tag="VFX21_11175"
     CDS             1070..2650
                     /locus_tag="VFX21_11175"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX5096570.1"
                     /translation="MSTDDHKDADHTAIISTAKGSGHLAGGSFFEFGSRFVIAVVLAR
                     TLGASDYGLYVLAVSAAGVFAGIASLGLDDAMTRHVAISSGRNDRAAIMGTLQIGFVG
                     AVGAGLVMAVVLFVAARPVASGWFDEPRLASLLRVVALAVPLLTVSNVLSGTARGFGK
                     MHFVAIAENGVQSIVRVVLTVAIVVLTGTISVMVATVAFVVSDVAATVVFVLLLVRHF
                     PVDRELQSNARRETRGVVMFALPLWVSSLLRQSRRNIQTVLLGTSRSVSSAGVFSIVD
                     KVNTVGHVWLLSLFVAVKPELARLHDRGDRRELGRLYATSTRWSLELTLPFVLVSVLF
                     REAILRIFGGSFEAGATALVILSCAELVNAATGVCGPAIDMTGHTRTKVANSVVFTAI
                     LIATNALLIPEWGVIGAAVAALLATTVFNALCIVEMWVFERLLPFDASSWKPLAAGAG
                     AFMCGLALDVLLPLDRVGAMVALAPVICAVYAGLLLSFGLPTEDRLVIARALSKIPRP
                     RRRRVTAGDTAAHDVAIG"
BASE COUNT          362 a          932 c          911 g          513 t
ORIGIN      
        1 caccggcgac cgttccctga ctcatgggaa gcacgctcgt gcacacggcg ccggtcgcac
       61 cgatcttcgt ggtcggagcg ccccgttccg gtacgacgat gctgcgctac atgctctgcg
      121 cccaccctcg gatctacatc cctcccgaat cgaacttcat tccccgcttc ttcggccgtc
      181 gcccgacgac gccactcgat cgtcgccgcg ccgtcgacat cgtccgcgag atcgagcggt
      241 accgcgtgtt cttcaaggac tggcacggcc cgctgccgga tccggcggag ctcgtcgacg
      301 agctgccgca gcccacgccc gccggcatca tcgacgcgct gtaccgccgc tatgcatcag
      361 agttcggcgc ggttcgctgg ggtgacaagt caccgatcta cacgcgctac gtcgacctcc
      421 tttcgacgat cttcccggcc gcgcggttcg ttcacatcgt gcgtgacggt cgcgatgtcg
      481 cgacctcgat gctgcacgcg tactcgacga gacgcttctt ctactacgac atctactacg
      541 cagcgcgcac atggcgtcac gatgtcggcg ccgcgcgcga gtcgggctcg cgcctgggcg
      601 cggcgcggta cagggaggtg cgctacgagg acctcgtcgc gagtccggtt gatcacctgg
      661 aggcgatctg tgcgtttctc ggagaacgat acgagtcggc gatggctcat cccgaagatg
      721 tcgcgcgccg ctcgtatcac tcccggggga tccacgccgc cacgcggcag ccggtgacga
      781 ctgagcgggc cgggacttgg cggacggggt tgtcgcggga cgacgtcgcg gtgttcgaca
      841 ccgtcgcggg ccggttgctg tccgagctgt gctacgaacc tcggggcggc tgcgccatga
      901 caccgcgcgc ccgagtcaac ttcgtacgtc ttcggtgcaa gtacacgggc gtcagcgccg
      961 gacgtgccgc gctccgggga gtcgggctgt tccacccgac cgagttgctc gcccgccacc
     1021 tccggctgcg tccctatacg cgcccacggg cctgttcggg tgccgctgga tgagcacgga
     1081 cgaccacaaa gacgcggacc acaccgcgat catctcgacc gcgaagggca gtgggcacct
     1141 cgcgggcggc agcttcttcg agttcgggag ccgattcgtc atcgccgtcg tgctcgcgcg
     1201 cacgctgggt gccagcgact acggcctgta cgtcctggcg gtgagcgcgg ccggcgtgtt
     1261 cgccggtatc gcgtcactcg gcctcgacga cgcgatgacg cgccatgtcg cgatctcgtc
     1321 cgggcgcaac gatcgtgccg cgatcatggg cacccttcag atcggtttcg tcggagcggt
     1381 cggtgccggt ctcgtcatgg ccgtggtgct cttcgtcgcg gcccgtccgg tcgccagcgg
     1441 gtggttcgac gaaccgcgcc tcgcctcgct ccttcgcgtc gttgcgttgg ccgtgccgtt
     1501 gctcacggtc agcaacgtgc tctccggcac cgcacgcggg ttcggcaaga tgcatttcgt
     1561 cgcgatcgcc gagaacggcg tccagtcgat cgtacgggtc gtgctgacag tcgccatcgt
     1621 cgtcctcacc gggaccatca gtgtcatggt cgcgaccgtc gcgttcgtcg tctcggacgt
     1681 cgcggcgacc gtcgtgttcg tcttgctgct cgtacggcac ttccccgtgg accgcgagct
     1741 gcaatcgaac gcccggaggg agacgcgcgg cgtggtcatg ttcgcgttgc cgctgtgggt
     1801 ctccagtttg ctccgacagt cacgccggaa catccagacg gtgctactcg gaacgtcgcg
     1861 cagcgtctcg agcgcgggtg tcttctcgat cgtcgacaag gtcaacacgg tcggccacgt
     1921 gtggctcctg tcgttgttcg tcgcggtcaa accggagctt gcccgcttgc acgatcgcgg
     1981 cgaccgacgc gagctcggcc ggctgtatgc aacgtcgacc cggtggtcgc tcgagctcac
     2041 gctgcccttc gtcctcgtga gcgtgctgtt ccgggaagcg attctccgca tcttcggcgg
     2101 gtcgttcgag gcgggtgcga ccgcgctggt catcctgtcc tgcgcggagc tcgtcaacgc
     2161 cgcgaccggt gtgtgcggcc cggccatcga catgaccggg cacacgcgca cgaaggttgc
     2221 gaattcggtg gtgttcaccg cgatcctgat cgcgacgaac gcgctgctca taccggagtg
     2281 gggtgtcatc ggagccgcgg ttgccgcgct gctggcaaca acagtgttca acgcgctgtg
     2341 catcgtggag atgtgggtgt tcgaacggct gctgccattc gacgcgtcgt cgtggaagcc
     2401 cctggccgcg ggggccggcg cattcatgtg cggtttggcg ctcgacgtgc tgctgccgct
     2461 ggaccgcgtc ggggcgatgg tcgcgctcgc gcccgtcatc tgcgctgtgt acgcggggtt
     2521 gttgttgtcc ttcgggctgc cgaccgagga ccgcctggtc atcgcgcgtg cgctcagcaa
     2581 gatcccgcgg ccgcgtcgcc ggcgtgtcac cgccggcgac accgcagcgc acgatgtggc
     2641 catcgggtga gcgctcctcc gcgggcgcgc acggggcccg aggcatcaca ccgttcgatc
     2701 agatcggcgc tcattcgc
//
LOCUS       DASUGA010000564         2436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_61765, whole genome shotgun sequence.
ACCESSION   DASUGA010000564 DASUGA010000000
VERSION     DASUGA010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61765"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..>2436
                     /locus_tag="VFX21_11180"
     CDS             133..>2436
                     /locus_tag="VFX21_11180"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX5096571.1"
                     /translation="MADAIRVTGPINAAGLEMTLETGKLAALADGAVTVKVGGVTLLS
                     TVCTSKPREGIDFFPLTVDVEERMYAAGKIPGSFFRREGRPGEQAILTCRLTDRPLRP
                     TFPSDFRNEVQVIATILGVDLENPHDVASINGASAALMVSGIPFNGPIGAVRLAHFDG
                     EWIAHPTFQQGEESTFEIVVAGRQLDDGDVAIMMVEAGGTENAWKLYEAGAPKVTEAV
                     IAQGLEESKQWIKASISLQLQLREAYEKAYGAIEPIEYLPALDYSEEIYDAVKQHAES
                     RTIEAMGIADKTDRNTRLAEVEAETLIALSGSADAPGRYPEEAQAVKKAFRSLQKNVV
                     RTRIVNDGFRIDGRSPGDLRPLSAEVGVVPTAHGTGLFQRGETQVLSVTTLGMPRMEQ
                     MLDTITLDDKKRYMHHYNFPPFSTGETGRVGSPKRREIGHGALAERALVPVIPDAQEW
                     PYTLRVVSDVLASNGSTSMASVCGSTLSLMDAGVPIKAPVAGIAMGLVYHDGKYTTLT
                     DILGAEDAFGDMDFKVAGTEEFVTALQLDTKIDGIPADVLAQALQQARDARMKILEVM
                     KGAIDAPREDVGPTAPKIVSFQIPIDKIGEVIGPKGKVINTIQQETGADISVGDEDGF
                     GIVSIGSKDGEAVREAQRRIELILDPPTAEVGAVYEGRVVNITKFGAFVNILPGRDGL
                     VHISKLGRGKRIDRVEDVLQLGDELEVRVDDIDQNGKLSLSLVGDEPEGDGDGGGDEG
                     APRPERRERSERSDRGNGGGDGREVASF"
BASE COUNT          444 a          804 c          828 g          360 t
ORIGIN      
        1 ctcctcgttt cgtatttgca atcggcgaga ccaagacgtc ggttgtcagt cgcgagcgcc
       61 ccggtcggca ccgaccgaga cgctgacaac tgggaaccga agcctctcgc ccacaaaaga
      121 gaggacgttt ccatggctga cgcaatccgc gtcacgggtc cgatcaatgc tgccggcctc
      181 gagatgacac tcgaaaccgg caaactcgct gcgctcgcgg acggcgcagt caccgtcaaa
      241 gtcggcggcg tcacgctgct gtcgacggtg tgtacatcca agccgcgtga aggcatcgac
      301 ttcttcccgc tgaccgtcga cgtcgaagag cgcatgtacg cggcgggcaa gatccccggc
      361 tcgttcttcc ggcgtgaagg ccggcccggc gagcaggcga tcctgacctg ccgcctcacc
      421 gaccgcccgc tgcggccgac gttcccgagc gacttccgca acgaggtgca ggtcatcgcg
      481 accatcctcg gcgtcgactt ggagaacccg cacgacgtcg cgtcgatcaa cggcgcgtcg
      541 gcggcgctga tggtgtccgg cattccgttc aacggcccga tcggcgcggt gcgcctcgcg
      601 cacttcgacg gcgagtggat cgcgcacccg acgttccagc agggcgagga gtcgacgttc
      661 gagatcgtcg tcgccggtcg tcagctcgac gacggcgacg tcgcgatcat gatggtcgag
      721 gccggcggga ccgagaacgc gtggaagctc tacgaagccg gcgcgccgaa ggtcaccgaa
      781 gcggtgatcg cgcagggtct cgaggagtcg aagcagtgga tcaaggcttc gatctcgctc
      841 cagctccagc tgcgtgaggc ctacgagaag gcgtacggcg cgatcgagcc gatcgagtac
      901 ctgcccgcgc tcgactactc cgaagagatc tacgacgcag tgaagcagca cgcggagtcg
      961 cgcacgatcg aggcgatggg catcgccgac aagaccgacc gcaacacgcg cctggccgag
     1021 gtcgaagcgg agacgctcat cgcgctctcc ggttccgccg acgcgccggg ccggtacccc
     1081 gaagaagcgc aggccgtgaa gaaggcgttc cggtcgttgc agaagaacgt cgtgcgcacg
     1141 cgcatcgtga acgacggctt ccgcatcgac ggccgcagtc cgggcgacct tcgtccgctg
     1201 agcgccgagg tcggtgtcgt cccgaccgcg cacggcacgg gcttgttcca acgcggtgag
     1261 acgcaggtgc tgtcggtcac cacgctcggc atgccgcgca tggagcagat gctcgacacc
     1321 atcacactcg acgacaagaa gcgctacatg caccactaca acttcccgcc gttctcgacc
     1381 ggtgagaccg gtcgcgtcgg ctcgccgaag cggcgggaga tcggtcacgg tgcactggcc
     1441 gagcgcgcgc tcgtcccggt catccccgac gcgcaggagt ggccgtacac gttgcgggtc
     1501 gtctccgacg tgctcgcctc gaacggttcg acgtcgatgg cgtcggtgtg cggttcgacg
     1561 ctgtcgctga tggacgccgg cgtgccgatc aaggcaccgg tcgcgggcat cgcgatgggc
     1621 ctcgtctacc acgacggcaa gtacacgacg ctcaccgaca tcctcggcgc ggaagacgcg
     1681 ttcggcgaca tggacttcaa ggtcgccggc accgaagagt tcgtcacagc actgcagctc
     1741 gacacgaaga tcgacggcat tcccgccgac gtgctggcgc aggcgctgca gcaggcgcgt
     1801 gatgcccgca tgaagatcct cgaggtgatg aagggcgcga tcgacgcgcc gcgcgaagac
     1861 gtcggcccga cggcaccgaa gatcgtgagc ttccagatcc cgatcgacaa gatcggcgag
     1921 gtgatcggac ccaagggcaa ggtcatcaac acgatccagc aggagaccgg cgccgacatc
     1981 tcggtcggcg acgaagacgg gttcggcatc gtgtcgatcg gttccaagga cggcgaggcg
     2041 gtgcgcgaag cgcagcgccg catcgagctg atcctcgacc cgccgaccgc ggaagtgggt
     2101 gcggtctacg agggtcgcgt cgtgaacatc accaagttcg gtgcgttcgt gaacatcctc
     2161 cccggccgcg acggtctggt gcacatctcg aagctcgggc gcggcaagcg catcgaccgc
     2221 gtcgaagacg tgctccagct cggcgacgag ctcgaggtgc gcgtcgacga catcgaccag
     2281 aacggcaagc tctcgctgtc gctcgtgggt gacgagcccg aaggcgacgg tgacggcggc
     2341 ggcgacgaag gcgcaccgcg tcccgagcgt cgtgagcggt cggagcgttc cgaccgtggc
     2401 aacggcggcg gcgacggccg tgaggtcgcg tcgttc
//
LOCUS       DASUGA010000565         2197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_97439, whole genome shotgun sequence.
ACCESSION   DASUGA010000565 DASUGA010000000
VERSION     DASUGA010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97439"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VFX21_11185"
     CDS             complement(<1..679)
                     /locus_tag="VFX21_11185"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX5096572.1"
                     /translation="MEGVRYMDVRERYRVRRGRARYVTLSLIAALAVAVPLVIAPWAG
                     SAPRKVTVYIGNSAGYAVGYRGPLGASLPRATALDSPDAMSSAYPVLVDINDDSQRDL
                     IVGNADGKIVVFENTNSDSAPAWTRRTDWEPANFDAGSTSSPAAIDFDRDGDKDLLVG
                     NSNGTLLALKNTGSDDAPAWATDPGRDWPSVGRYSHPAVADVNKDGKPDVLVGLSTGV
                     VIGFTGSG"
     gene            936..1259
                     /locus_tag="VFX21_11190"
     CDS             936..1259
                     /locus_tag="VFX21_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX5096573.1"
                     /translation="MTTVPSAEIAHGTVRRVDTADGEFVVWRGGDGRVRSAPRWCPHL
                     DHDLSEGYVAGNELVCPGHAWAFDGDGRAYKRNEFGRVDPKGSVPVLVVREADAMIRF
                     DHDPV"
     gene            1266..2099
                     /locus_tag="VFX21_11195"
     CDS             1266..2099
                     /locus_tag="VFX21_11195"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEX5096574.1"
                     /translation="MTSEPTTEPIAYPPEQHLLRDLRFSFEHDEPGRASRAWLPIVPE
                     LCTDHGHARVGPLATLVDVIGGGLAANAAAPDWIATADLTIHLVRGAPAGSTVEARAA
                     VQRKGRTTVVMDVQLLDDDERELGIATMSFSVLPRRDTNPTMETMNTGPTTMARPESR
                     LPAPIPEMLAIDVRDAARGELDVPVTDWCRNSMGAMQGGVVAMVVECAAETALRAAGE
                     PNMVVADLELTYLGFGRVGPIRTHVDVLGRDAAHVELVDTGADPRRMTLARATGTRGL
                     A"
BASE COUNT          368 a          733 c          756 g          340 t
ORIGIN      
        1 cgcccgaccc ggtgaagccg atgacgacac cggtggagag cccgacgagc acgtcgggct
       61 tgccgtcctt gttcacgtcg gcgactgcgg ggtgcgagta gcggccgacg ctcggccagt
      121 cacggccggg atcggttgcc cacgccggcg cgtcgtccga gcccgtgttt ttgagcgcga
      181 gcaatgtgcc gttcgagttt ccgacgagca agtctttgtc gccgtcacgg tcgaagtcga
      241 tcgcggctgg tgatgacgtc gagccggcgt cgaagtttgc cggttcccaa tcggtgcgcc
      301 gcgtccacgc cggggcgctg tccgagttgg tgttctcgaa cacgacgatc ttgccgtcgg
      361 cgttgccgac gatgagatcg cgttggctgt cgtcgttgat gtcgacgaga acgggatacg
      421 cactcgacat cgcgtcgggc gagtcgagcg cagtggcgcg cggcaacgac gcgccgagcg
      481 gaccgcggta gccgaccgcg tagcccgcgg agttcccgat atagaccgtc actttgcggg
      541 gcgcggaacc ggcccatggc gcaatgacga gcggaactgc gaccgccagc gcggcgatca
      601 acgaaagcgt gacatagcgc gcgcgtccac gacggacgcg ataacgctcg cgaacatcca
      661 tgtaacgaac cccctccatg ggaagacgtc ccttttcccc acgacgcaac cggccctcaa
      721 ctccggtgcg ccaacttgcg cggaacgtcc gaatcgggac gatatcagga cgttttgcag
      781 ccccgccaac gcgcgcgatc cccacgtgaa ttccccccgt tcaacccccg aacgcctgcc
      841 ccacccctct tgatcggcgg aggcccggtg ctttctgaaa cgaacccgga aaaagccgtt
      901 cgcgagaatg agcagatgtc tcgccgagcc gcctcgtgac gacggttccg agcgcggaaa
      961 ttgcccacgg gacggtccgg agggtggata cggccgacgg cgagttcgtc gtgtggcgcg
     1021 gtggcgacgg acgggtgcgc agcgcgccgc gttggtgtcc gcatctcgac cacgacctct
     1081 ccgagggcta cgtcgccggc aacgagctgg tgtgtcccgg gcacgcctgg gcgttcgatg
     1141 gcgatggtcg cgcgtacaag cgcaacgagt tcggtcgcgt cgatccgaag ggcagcgtgc
     1201 cggttctggt cgtgcgcgaa gccgatgcca tgatccggtt cgaccatgat ccggtttgac
     1261 ttgccatgac atccgagccg acgaccgaac cgatcgcgta tccacccgag cagcatctgc
     1321 tgcgagacct ccgattctcg ttcgagcacg acgagccggg gcgggcaagc cgcgcgtggc
     1381 tgccgatcgt tccggagctc tgcaccgatc acggtcacgc acgcgtcggt ccgctcgcga
     1441 cgctcgtcga cgtgatcggc ggcggtttgg cggcgaacgc ggcggcaccc gactggatcg
     1501 cgacggccga cctgacgatt catctcgttc ggggcgcgcc cgcaggctcg acggtcgagg
     1561 cccgggccgc ggtgcaacgc aaaggccgca cgaccgtcgt gatggacgtg caactcctcg
     1621 acgacgacga acgggagctc ggcatcgcga ccatgagctt ttcggtgctc ccccgccgcg
     1681 acacgaatcc gacgatggaa acgatgaaca ccggcccgac cacgatggcc cggccggagt
     1741 cgcgattgcc ggcgccgatc cccgagatgc tcgcaatcga cgtacgcgat gcggcacgcg
     1801 gcgagctaga cgtgccggtc accgactggt gccgcaactc gatgggcgcg atgcaaggtg
     1861 gcgtcgttgc gatggtcgtc gaatgtgcgg ccgagacggc gctgcgcgcc gcgggcgaac
     1921 cgaacatggt cgtcgccgac ctcgaactga cctatctcgg tttcggccgg gtcggtccga
     1981 tccgtacgca cgtcgacgtg ctcggccgcg acgccgcgca cgtggaactc gtcgacacgg
     2041 gcgccgaccc gcgccgcatg acgctcgcgc gcgcaaccgg aacgcgagga ctggcgtgaa
     2101 ggggttcacc ttcgatggcc cgcgtaacga gcggccgatc gcgggccggg aggtcggccg
     2161 ctacgtggga ctcgacctgc gcgagaccga ggagggg
//
LOCUS       DASUGA010000566         2669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1222554, whole genome shotgun sequence.
ACCESSION   DASUGA010000566 DASUGA010000000
VERSION     DASUGA010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222554"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1726)
                     /locus_tag="VFX21_11200"
     CDS             complement(<1..1726)
                     /locus_tag="VFX21_11200"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEX5096575.1"
                     /translation="MSRTIRAVATLVVVGIITTVAPLVRAVPASAARPKTIAIADAAI
                     VEGDAGTKSLAFNVTWTGAKGGGAVSVAYATADGTATAGSDYTAKSGTVSLPSGCKCG
                     TISIPIVGDAVTEGTETFTVNLSLAVNATIGDSQAVGTIFDNEGPAAFVILDGSANEN
                     AGPVAFSVIMTNSSGTTQTVDYATSDGSATAGADYTATSGTLTFTAGQTSKTVNVPVL
                     NDTLNEADEMFTLTLSNTTLALNDATAAGTITDDDAEPTVSIGDAGAPEGDGTVSFTV
                     SLSALSGQEVDVDYATTDGTALAGSDYVAATGTAVIPAGSASTTIDVTVTDDTTYEND
                     EGFTIEISSPFNASIVDAQGAGTIANDDALPSASIGDVSVAEGTAGSTPAGFTVTLSN
                     PSAFTATVDWATADGSATEGSDYTGAGGTLTFDPGVTSQPVSVDVLGDTMFESDETFT
                     VTLSNPTGSTIGSGTGTGTITNDDKAVTTLTLAITKTKAKVGAKGVLERATADASVTV
                     TIYRRHHRHWVKLSTKTVGVRSLGDRDADATPDATYRAGFPRPTHGTYRFVATFAGNA
                     SLLPSSSKT"
     gene            1864..2277
                     /locus_tag="VFX21_11205"
     CDS             1864..2277
                     /locus_tag="VFX21_11205"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HEX5096576.1"
                     /translation="MHRVLTFLRMPPADRALAVRAAGWLLVARVALWTMPFPRVQDLA
                     ARMGAGRRTVGVAPGRVAWTVETVARSIPEATCLTQALAADVMLSRMGEEPSIHIGVA
                     TDRGAFEAHAWLELHGRPLVGDVELDRYARLEPGR"
     gene            complement(2463..>2669)
                     /locus_tag="VFX21_11210"
     CDS             complement(2463..>2669)
                     /locus_tag="VFX21_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096577.1"
                     /translation="VAPVPIGSQRGYHALMSVELQGVEVAVDFNVLFHGTQEYTIVCQ
                     STPSATAVVAEGCETIRSTFEVTG"
BASE COUNT          334 a          917 c          971 g          447 t
ORIGIN      
        1 ccgtcttcga cgaggagggc aacagcgacg cgttgcccgc gaacgtcgcg acgaaccggt
       61 acgtgccgtg cgtgggccgc gggaagccgg cccggtaggt cgcgtccggc gttgcgtccg
      121 catcgcggtc gccgagggag cgcacaccca ccgtcttcgt cgagagcttg acccagtggc
      181 ggtggtgcct gcggtagatc gtgacggtca cgctcgcatc cgcggtcgcc cgctcgagga
      241 cgccctttgc gccgaccttc gccttggtct tcgtgatcgc gagcgtcagc gtcgtgaccg
      301 ccttgtcgtc gttcgtgatc gtccccgtgc ccgtcccgct cccgatggtg gatcccgtcg
      361 ggttcgacag cgtgacggtg aacgtctcgt cggactcgaa catcgtgtcg ccgagcacgt
      421 cgaccgacac gggctgcgag gtcacgcccg ggtcgaacgt cagcgtgccg ccggcgcccg
      481 tgtagtcgga gccctcggtc gccgagccgt ccgcggtcgc ccagtccacc gtcgcggtga
      541 acgccgacgg gttggacaac gtgacggtga agccggccgg cgtcgacccc gccgtgccct
      601 ccgcgaccga gacgtcgccg atcgaggcgg acggcaacgc atcgtcgttc gcgatcgtgc
      661 cggcgccctg tgcgtcgacg atcgaggcgt tgaacggcga ggagatctcg atcgtgaatc
      721 cttcgtcgtt ctcgtacgtc gtgtcgtcgg tgaccgtgac gtcgatggtc gtcgacgccg
      781 atcccgccgg gatgacggcc gtcccggtcg ccgcgacgta gtcggagccc gcgagcgccg
      841 tgccgtcggt cgtcgcgtag tcgacgtcga cctcctggcc gctcagggcc gacagcgaca
      901 ccgtgaagct cacggtgccg tcgccctcgg gcgcgccggc gtcgccgatc gacacggtgg
      961 gctcggcgtc gtcgtccgtg atggtgccgg ccgcggtcgc gtcgttcagc gccagcgtcg
     1021 tgttgctcag ggtgagggtg aacatctcgt cggcctcgtt cagcgtgtcg ttgagcaccg
     1081 gaacgttgac ggtcttcgac gtctgtcccg ccgtgaacgt cagcgtgccg ctcgtcgcgg
     1141 tgtagtcggc gccggcggtc gccgagccgt cgctcgtcgc gtagtcgacg gtctgggtgg
     1201 tgccggacga gttcgtcatg atcacgctga acgcgaccgg cccggcgttc tcgttggcgc
     1261 ttccgtcgag gatcacgaac gccgccggcc cctcgttgtc gaagatcgtc ccgaccgcct
     1321 gcgagtcgcc gatcgtcgcg ttgaccgcca acgacaggtt gaccgtgaac gtctccgtgc
     1381 cctcggtgac cgcgtcaccg acgatcggga tcgagatcgt gccgcacttg cagccgctcg
     1441 gcagcgagac ggtgccggac ttcgccgtgt agtccgagcc ggccgtcgcc gtgccgtcgg
     1501 cggtggcgta ggcgaccgag acggccccgc cgcccttggc cccggtccac gtgacgttga
     1561 acgcgaggga cttcgtgccg gcgtcgcctt ccacgatcgc ggcgtcggcg atggcgatcg
     1621 tcttcggcct ggcggccgac gcgggcaccg cgcggacgag cggggccacg gtggtgatga
     1681 tgccgacgac gacgagcgtc gcgacggcgc ggatcgttcg agacacggtt gccctccggg
     1741 gtctggggag tatgggtgac gcgttcatcc acccatcggc ggtccaccgg ccgggggaca
     1801 tacccgggcg cacgagttgc cgctaccccg cacgcggtac ggcgtctgga tacagtcgtc
     1861 gccgtgcatc gggttctcac gttcctgcgg atgcccccgg cggatcgcgc tctggcggtc
     1921 cgcgcagccg gctggttgct cgtggcgcgc gtcgcgttgt ggacgatgcc gttcccgcgc
     1981 gtgcaggacc tggccgctcg gatgggcgcc ggccgccgta ccgtcggcgt ggcgcccggc
     2041 cgggtggcgt ggaccgtcga gacggtcgcg cggtcgatcc cggaggcgac gtgcctgacg
     2101 caggcgcttg cggccgacgt gatgctgtcc cggatggggg aggagccgtc gatccacatc
     2161 ggcgttgcga ccgaccgcgg cgcgttcgag gcgcacgcgt ggttggagct gcacggcagg
     2221 ccgctggtcg gcgacgtcga gctcgatcgg tacgcgaggc tcgagccggg gcgctaaccg
     2281 cgcgggccgc cggtctgtgt ccgccggtcg atcgcgcgcc cggtcgatcg ctcggcgtca
     2341 acgggttgac gcgcgagggg ccggggcgcc acgcggaatc ttgtcaacga gttgtcgtga
     2401 ttgaacgggc aagcagttga cgtgttcgcg ccggacccac acgcggcgcg tcggccctcc
     2461 agctagccgg tcacctcgaa ggtgctgcgg atcgtctcgc atccttccgc cacgacggcc
     2521 gtcgcggacg gcgtcgactg acacacgatc gtgtactcct gcgtcccgtg gaacagcacg
     2581 ttgaagtcca ccgcgacctc gacgccctgg agctcgaccg acatcagcgc gtggtacccg
     2641 cgctgcgaac cgatggggac cggcgcgac
//
LOCUS       DASUGA010000567         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1382004, whole genome shotgun sequence.
ACCESSION   DASUGA010000567 DASUGA010000000
VERSION     DASUGA010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382004"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..>2020)
                     /locus_tag="VFX21_11215"
     CDS             complement(152..>2020)
                     /locus_tag="VFX21_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX5096578.1"
                     /translation="ATANAAGGANGTGAPKRRRRGSRGGRRRKKPAGAGGASNGNANG
                     AARAPRANEHDDESGEDWSDAAADRGLTSEDLADDAREDAGLVRERPPNAPRVGDSRP
                     APAARPAVGDSRAPAPAADPDAAPKKRRRRRGGRGRSKSGGGNGGGNGGGRTAAGGTA
                     YVDAGVALSDVDVDGTAILEQLDEETLERRRGLHRKGRPAGRYLMVVHERPDGMSHIA
                     VLEGRSLVELYVSLPTDDTTSIDGNIYLGRVQNVLPGMEAAFIDIGTPKNGVLYRGDV
                     VYDESEVEGGDRARIERVLRNGQAITVQVTKNPIGHKGARLTQEVSLAGRFVVMVPNQ
                     PNTYGISKRLPDDERKRLRRILDRLRPADAGLIVRTAAEGATDDELERDMRRLNSQWQ
                     QISQIAKSAKPGSLLYKEPPLAIRLIREEFTKEYRGVVIDSPQLYEEVRSYVDAMAPE
                     LADRVELFDEEAEHLPIFEKFHIHEQIHKALDRKVWLPSGGSIIVERTEALTVIDVNT
                     GKNVGASNLEETVFKNNLEAAEAVARELRLRDIGGIIVIDFIDMESKSNREQVEEAFR
                     QALARDKTRTQVFPISELGLLEMTRKRVSEGLVEVFSSTCPHCNGRGFVVDETMLE"
BASE COUNT          272 a          706 c          687 g          355 t
ORIGIN      
        1 cgagcacgcg atcgagatcg cggcgaaggc cggcgcccgg cgcctgatcg tgacgcacca
       61 cggcccgttc cgcaccgacg acgcggtcac cgccatcgcg ggccgcctcg gattggaaac
      121 cgcctactaa ggcctcgccg tcgacctcta actattcgag catcgtctcg tcgacgacga
      181 agcctcggcc gttgcagtgg ggacacgtcg acgagaagac ctcgacgagg ccttcggaga
      241 cgcgcttgcg cgtcatctcc aacaggccca gctccgagat cgggaacacc tgggtgcgcg
      301 tcttgtcacg cgcgagcgct tggcggaacg cttcttcgac ttgttcgcgg ttcgacttcg
      361 actccatgtc gatgaagtcg atgacgatga tgcccccgat gtcacgcagg cgcagctcgc
      421 gcgccacggc ctccgccgct tccaagttgt tcttgaagac cgtctcttcg aggttcgacg
      481 caccgacgtt cttgccggtg ttgacgtcga tgaccgtcag cgcctcggtg cgctcgacga
      541 tgatcgaacc gcccgacggg agccacacct tgcggtcgag cgccttgtgg atctgctcgt
      601 ggatgtggaa cttctcgaag atcggcaggt gctcggcctc ttcgtcgaac agctcgacgc
      661 ggtccgcgag ctcgggcgcc atcgcgtcga cgtaggaacg cacctcttcg tagagctggg
      721 gtgagtcgat gacgacaccg cggtattcct tggtgaactc ttcgcggatc aaccggatcg
      781 cgagcggcgg ctccttgtag aggagactgc cgggcttcgc gctcttggcg atctgcgaga
      841 tctgctgcca ctggctgttc agccggcgca tgtcgcgttc gagctcgtcg tcggtggcgc
      901 cttcggccgc ggtgcggacg atcaagcccg cgtccgcggg tcgcagccgg tcgaggatgc
      961 gccgcaaccg cttgcgttcg tcgtcgggaa ggcgcttgga gatgccgtac gtgttcggct
     1021 ggttcggcac catcacgacg aaacgaccgg ccagactcac ttcctgcgtg agccgcgcac
     1081 ccttgtggcc gatcgggttc ttggtgacct gcaccgtgat cgcctgaccg ttgcgcagca
     1141 cccgttcgat gcgggcgcgg tcgccgcctt cgacctcgga ctcgtcgtag acgacgtcgc
     1201 cgcggtacag cacgccgttc ttgggcgtgc cgatgtcgat gaacgcagct tccatgccgg
     1261 gcaggacgtt ctgcacgcgg cccaaataga tgttgccgtc gatcgatgtc gtgtcgtcgg
     1321 tcggcagcga cacgtacaac tcgacgagcg agcggccttc gagcaccgcg atgtgcgaca
     1381 tgccgtcggg ccgttcgtgc acgaccatca ggtatcgacc ggcaggacga cccttgcggt
     1441 gcagaccgcg gcgacgctcg agcgtctcct cgtcgagttg ctcgaggatc gcggtgccgt
     1501 cgacgtcgac gtcggagagt gcgacaccgg cgtcgacgta cgccgtgccg ccggctgcgg
     1561 tgcgaccgcc gccgttgccc ccgccattgc caccacccga cttggaccga ccgcgtccgc
     1621 cacggcgacg gcgccgcttc ttgggcgcag cgtcgggatc ggcggccggt gccggcgcgc
     1681 gcgagtcgcc gaccgcgggc cgggctgcgg gcgcgggccg ggagtcaccg acgcgcggcg
     1741 cgttcggggg ccgctcccgg accaagcccg cgtcttcgcg agcgtcgtcg gcgaggtcct
     1801 cggacgtcag gccgcggtcg gcggccgcgt ccgaccagtc ctcgcccgat tcgtcgtcgt
     1861 gctcgttcgc gcgaggcgca cgcgccgcgc cgtttgcgtt gccgttgctc gcgccgcccg
     1921 cgccggccgg cttcttgcgg cggcggccac cccgcgaacc gcgacggcgg cgcttcggcg
     1981 cgcccgttcc gttcgcgccc cccgccgcgt tggccgtggc
//
LOCUS       DASUGA010000568         5079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_292742, whole genome shotgun sequence.
ACCESSION   DASUGA010000568 DASUGA010000000
VERSION     DASUGA010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5079
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292742"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /gene="cysS"
                     /locus_tag="VFX21_11220"
     CDS             complement(<1..1027)
                     /gene="cysS"
                     /locus_tag="VFX21_11220"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX5096579.1"
                     /translation="MSASPDQTALEPMRLFDTARQKVVPFEPGDIVTMYTCGITPYDA
                     THLGHAAVYLTYDVLQRRLIDRGHSTRCVRNVTDVDDDILRKARELGVHYLDLAAAET
                     ARFDADMRALDVMECWSEPRATSAIADIRGFIGMVLDRGHAYVSNGAVYFDVSSFKRF
                     GQVSHLPREEMLRLAAERGGNVDDPNKRDPLDFVLWQPSAEDEPSWESLWGPGRPGWH
                     IECSALALRELDTTIDLHGGGSDLIFPHHECEAAQSEAATGQPFVRHWMHQAMVRMDG
                     EKMSKSLGNLVFVSDLLKTFDARAIRLACVSHHYRDSWEYDDSLLPANADRLDRWMAT
                     AASPSARR"
     gene            complement(1085..1747)
                     /gene="npdG"
                     /locus_tag="VFX21_11225"
     CDS             complement(1085..1747)
                     /gene="npdG"
                     /locus_tag="VFX21_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEX5096580.1"
                     /translation="MQIGILGATGPAGSGLAARLASVGFDVVVGSRSKYRAMEVRDAL
                     LERWPGRSLAVEAADNEGAAAADVVVIATPWDAAAATAKSVDVQLQGKVVISMANALA
                     KVGHEFQPLVPPRGSVAASVQAAVPRSRVAAALHHVPAKELGDLDAPVESDVLVCSDH
                     PAATETTLDIVSKIPDLRPLDAGELSNAAPIEAFAAVLLQLNIRYRTRAAVRFTGIQV
                     DH"
     gene            1851..2618
                     /gene="fabI"
                     /locus_tag="VFX21_11230"
     CDS             1851..2618
                     /gene="fabI"
                     /locus_tag="VFX21_11230"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX5096581.1"
                     /translation="MGLLEGKRLLITGVLTDASLGFGVAQLAQREGAEIVITSVGKAH
                     KHTERAARKLPTEPDIIEFDVADAAHPAIVREQLAAKWDRVDGALHAIGFAPPVCLGG
                     TFLDAEWDDVGVALNVSAYSLKAVADAVVPLMHDGGSIVGLDFDAAVAWPAYDWMGVA
                     KAALESTARYLARYLGSQGIRVNLVAAGPIKTIAARSIPGFSKFEDVWADRAPLGWNV
                     KDSDAVARACVALLSDWFPMTTGEIVHVDGGYHAVGA"
     gene            complement(2627..2986)
                     /locus_tag="VFX21_11235"
     CDS             complement(2627..2986)
                     /locus_tag="VFX21_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6285 domain-containing protein"
                     /protein_id="HEX5096582.1"
                     /translation="MSGLHDRPDVIELVAAVREFLEQDVLAATEGRVQFHTRVSINAL
                     HMVERELAERDAMEAAHAVRLRQLGVTSDEELSAAIQDGQFDDRWEELSAAVYESVIE
                     KLRVANPDYLLDDEAEG"
     gene            complement(2983..3954)
                     /locus_tag="VFX21_11240"
     CDS             complement(2983..3954)
                     /locus_tag="VFX21_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX5096583.1"
                     /translation="MAPSTGDIAEPLGAFLRGVVDDVRRLSGGASRETFAFTLTKPGE
                     TEGVPLILQRVRSSTLASGFSMVAEADLLRAAGASGVPVAPVVDASDDASIVGAPFIV
                     VERLPGETIARRILRDDAYAVARERLIEQSAQALARVHAIDPSSVGNLKREDQLSSVL
                     SIYDMLTPDMGTHPAFDIGFRELERSRPESQREVVLHGDYRLGNLLVDEQGLVAALDW
                     EIAHLGDPLEDLAWFSIRAWTFGGPGEVAGVGTVDELLGAYEAASGTTVDVDAFRWWR
                     AFETLKWGVICMLQAHTHLSGASRSVELATIGRRVCENEYDLLRLLR"
     gene            complement(3956..>5079)
                     /locus_tag="VFX21_11245"
     CDS             complement(3956..>5079)
                     /locus_tag="VFX21_11245"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX5096584.1"
                     /translation="SRPVRSRIPSTALRRAGARGAVVALVALALVGVSLPASAQSTPN
                     LADARRAADAAAARVQQLQAELGELDGELARLESEQEAAQAELDRLRDQVQHVAIDRY
                     TRVGTDDVVLLAEDFNEGERARALAEIVTQGDRDALDRYAATKERLARTSKEVEARRG
                     EQQKRLSALEQEQQRMADELERLEQLEAQRLEAERRAAEEAARRNDEAAKQRAAQLAA
                     LADARSAGKAGGGAPAVSSGPPRQVASGDWVCPVQGARTFRDSWGDARSGGRAHKGVD
                     IMSPRGTPVVNPVSGVVSTRGDRLGGLSMHVQGDDGNYYYGTHLDSYSGATGHLPAGT
                     VVGYVGDTGNAQGTHLHFEIHIGGYGNPINPYPTVARYC"
BASE COUNT          793 a         1803 c         1673 g          810 t
ORIGIN      
        1 cgcgacgcgc cgacggcgag gccgctgtgg ccatccaccg atcgaggcgg tcggcgttcg
       61 caggcagcaa cgagtcgtcg tactcccacg agtcgcggta gtggtgcgac acgcacgcca
      121 gccggatggc gcgagcgtcg aacgtcttca acaggtcgct cacgaacacc aggttgccga
      181 gcgacttcga catcttctcg ccgtccatcc gcaccatcgc ctggtgcatc cagtgacgaa
      241 cgaagggctg accggtggcc gcctcggatt gggccgcttc gcactcgtgg tgcgggaaga
      301 tcaggtcgga tccaccgccg tgcagatcga tcgtggtgtc gagctctcgc agggccagcg
      361 ccgaacactc gatgtgccag cccggtcggc ccggacccca cagcgactcc caggacggtt
      421 cgtcttcagc ggacggctgc cacaacacga agtcgagcgg gtcccgcttg ttggggtcgt
      481 ccacgttgcc accccgctcc gccgccaacc gcagcatctc ctcacgaggg agatggctca
      541 cctgaccgaa ccgcttgaag ctgctcacgt cgaagtacac cgcgccgttg ctcacgtacg
      601 cgtgtccgcg gtcgagcacc atgccgatga agccgcggat gtcggcgatc gccgacgtcg
      661 ccctcggttc gctccagcac tccatgacgt cgagcgcacg catgtcggcg tcgaagcgcg
      721 ccgtctcggc cgcagccaag tcgaggtagt gcacgcccag ctcgcgcgcc ttgcgcaaga
      781 tgtcgtcgtc gacgtcggtg acgttgcgca cacatcgtgt cgagtggccg cgatcgatca
      841 ggcggcgttg cagaacgtcg taggtgaggt agaccgctgc gtgtccgagg tgcgtcgcgt
      901 cgtacggtgt gatcccgcac gtgtacatgg tgacgatgtc gcccggctcg aacggcacca
      961 ccttctgccg agcggtgtcg aacaaccgca tcggctcgag ggccgtctga tcgggcgagg
     1021 cagacatcgg agcgtcaggc taccggccgc cagaacaacc agacgtggcc tcgcaaccgc
     1081 ctggtcagtg gtcgacctgg atgcccgtga accgcaccgc agcccgtgtg cggtaccgga
     1141 tgttcagctg caacaacacg gcagcgaagg cctcgatggg tgccgcgttc gagagctcgc
     1201 cggcatcgag gggtcggaga tcggggatct tcgagacgat gtcgagggtg gtctcggtgg
     1261 cggccggatg atcggagcac acgagcacgt cgctctcgac cggtgcatcg agatcaccca
     1321 gctccttggc gggcacgtgg tgcagcgccg cggccacgcg cgaccggggc acggccgctt
     1381 gcacgctggc cgcgaccgaa ccacgcggcg gcacgagtgg ctggaactcg tggccgacct
     1441 tggccagcgc gttggccatc gagatcacga ccttgccctg caattgcacg tcaaccgact
     1501 tggccgtggc ggctgctgca tcccatggcg tggcgatcac caccacgtcg gccgcggccg
     1561 cgccctcgtt gtcggccgcc tccacggcca ggctgcgtcc cggccaccgc tcgagcagcg
     1621 cgtcgcgcac ttccatggcg cggtacttcg accgcgagcc gacgaccacg tcgaagccga
     1681 ccgacgcgag tcgcgccgcc aagccactcc ctgctggacc cgttgcaccc aagatgccga
     1741 tctgcacggc ggcaaatgta gtgcttgctg gacgagcacc gtccaaacga tgcgaacgtg
     1801 accgctgacc atgtcggtaa cgtgcacccc ggacgaggag tcggaggacc atgggtctcc
     1861 tggaaggcaa gcgattgctc atcaccggcg tgctgaccga cgcgtcgctc ggcttcggtg
     1921 tggcccagct cgcacagcgc gagggcgcgg agatcgtcat cacgagcgtc ggcaaggccc
     1981 acaagcacac ggaacgagcg gcgcgcaagc tgccgaccga gcccgacatc atcgagttcg
     2041 acgtcgccga tgctgcacat ccggcgatcg tgcgcgagca actcgcggcc aagtgggatc
     2101 gcgtggacgg cgcattgcat gccatcggct tcgcaccgcc agtgtgtctg ggtggcacgt
     2161 tcctcgatgc cgagtgggat gacgtcggcg ttgccctcaa cgtgtcggcg tactcgttga
     2221 aggctgtggc cgacgccgtc gtgcccctga tgcacgacgg cggttccatc gtcgggctcg
     2281 acttcgatgc cgcggtcgcg tggcccgcgt acgactggat gggcgtggcc aaggccgcgc
     2341 tggagtcgac ggcgcgctac ctcgcccgct acctcggctc gcagggcata cgggtcaacc
     2401 tcgtcgccgc cggccccatc aagacgatcg cggcgcgctc gatccccgga ttctcgaagt
     2461 tcgaggacgt ctgggccgat cgcgcgccac tcggctggaa cgtgaaggac agcgatgccg
     2521 tggcccgcgc atgcgtcgcg ctgctctccg actggttccc catgaccacc ggtgagatcg
     2581 tgcacgtcga cggcggctac cacgccgtcg gcgcgtagat cgcgcgtcag ccctcggctt
     2641 cgtcgtcgag caggtagtcg gggttggcca cgcgcagctt ctcgatcacc gactcgtaga
     2701 cggcggccga cagctcctcc caccgatcgt cgaactgccc gtcctggatc gcggccgaga
     2761 gctcttcgtc actcgtcaca ccgagctgcc ggagccgcac cgcgtgggct gcctccatcg
     2821 catcgcgctc ggccagctcc cgttccacca tgtgcagggc gttgatcgac acccgggtgt
     2881 ggaactgcac gcggccttcg gttgccgcga ggacgtcctg ctcgaggaac tcgcgcacgg
     2941 ccgcgaccag ttcgatgaca tcaggtcgat cgtgcagacc gctcatcgca gcaaccgcag
     3001 caggtcgtat tcgttctcgc acacccgccg cccgatggtg gcgagctcga ccgatcgcga
     3061 cgctcccgac agatgggtgt gcgcctggag catgcagatc acgccccact tcaacgtctc
     3121 gaaggcgcgc caccatcgga aggcatcaac gtcgacggtc gtgccacttg cagcctcgta
     3181 cgcgccgagc aactcgtcga ccgtgcccac acctgccact tcgcccggcc caccgaaggt
     3241 ccacgcgcgg atggagaacc acgcgaggtc ctccaacgga tcacccaggt gcgcgatctc
     3301 ccaatcgagg gcggccacca gcccttgctc gtcgacgagc aggttcccca accggtagtc
     3361 gccgtgcagc acgacctctc gctgactctc gggacggctt cgctccagct cgcggaaccc
     3421 gatgtcgaac gcggggtgtg tgcccatgtc gggcgtcagc atgtcgtaga tgctgagcac
     3481 tgacgagagc tgatcctcgc gcttcaagtt gcccaccgac gaaggatcga tcgcgtgcac
     3541 gcgcgccagg gcttgcgcgc tctgttcgat caaccgttcg cgtgcgacgg cgtacgcgtc
     3601 gtcgcgcaag atccgtcgcg cgatcgtctc accgggcaag cgctcgacga cgatgaacgg
     3661 cgcaccgacg atcgacgcgt catcggacgc gtccacgacc ggcgcgaccg ggacgccgct
     3721 cgcgcccgcg gcgcgcagca gatcggcctc ggcgaccatg gagaacccgc ttgccaacgt
     3781 gctcgaacgg acgcgctgga ggatcagcgg cacgccttcg gtctcgcccg gcttcgtcag
     3841 cgtgaacgcg aacgtctcgc gcgacgcccc acccgacagc ctgcggacat cgtcgacgac
     3901 gcccctgagg aacgcgccga gcggttcggc gatgtcgccc gtggagggcg ccacgtcagc
     3961 agtaccgagc gacggtgggg tacggattga tcggattgcc gtacccgccg atgtggatct
     4021 cgaagtgcag gtgcgtgccc tgtgcgttgc ccgtgtcgcc gacgtatccg accaccgtgc
     4081 cggcgggaag gtgcccggtt gcacccgagt acgagtcgag atgggtgccg tagtagtagt
     4141 tgccgtcgtc gccctgcacg tgcatcgaca ggccaccgag ccgatcgcct cgcgtcgaga
     4201 ccacaccgct caccggattg acgacgggtg tgccccgcgg cgacatgatg tcgacgccct
     4261 tgtgggcccg accacccgaa cgagcgtcac cccagctgtc gcggaacgtc cgtgcgccct
     4321 ggaccgggca gacccagtcg ccggacgcga cctgacgggg cggtccggac gacacggccg
     4381 gcgcaccgcc accggccttc cccgcgctgc gcgcgtcggc cagcgcggcc agctgcgcgg
     4441 cccgttgctt ggccgcctcg tcgttgcgtc gggccgcttc ctcggctgct cgtcgctcgg
     4501 cttcgagccg ctgcgcttcg agttgctcca gccgttccag ctcgtcggcc atgcgctgct
     4561 gctcttgttc cagcgcggag aggcgcttct gctgctcgcc tctgcgggct tcgacttcct
     4621 tgctggtgcg cgccaaccgc tccttggtgg ccgcgtagcg gtcgagcgcg tcgcgatcgc
     4681 cctgcgtcac gatctcggcg agggcgcgcg ctcgctctcc ctcgttgaag tcctcggcca
     4741 gcaacacgac gtcgtcggta cccactcgcg tgtaccgatc gatggcgacg tgctgcacct
     4801 ggtcgcgaag ccggtcgagc tcggcctgtg ccgcctcctg ttcggattcg aggcgcgcca
     4861 gctcgccgtc gagctcaccg agctcggctt ggagctgctg cacgcgagcc gcggcggcat
     4921 cggccgcacg tcgcgcatcg gccagattcg gtgtggactg cgcactcgcc gggagcgaca
     4981 cccccaccaa cgccagcgcc acgagcgcaa cgaccgcgcc tcgggcaccg gcgcgtcgca
     5041 acgccgtgga ggggatccgg ctccgcacgg gccgagact
//
LOCUS       DASUGA010000569         2686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1266095, whole genome shotgun sequence.
ACCESSION   DASUGA010000569 DASUGA010000000
VERSION     DASUGA010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266095"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VFX21_11250"
     CDS             complement(<1..682)
                     /locus_tag="VFX21_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEX5096585.1"
                     /translation="MADTDPSSGARRIPEATVARLPLYYRALLETAERQVATISSERL
                     AELAGVNAAKVRKDLSYLGSYGTRGVGYDVEYLLHEISRELGLTRDWPVVIVGIGNLG
                     RALANYRGFGARGFRVVALVDADPDLVGGEVGELAIEAMDDLDQIVADRQIAIGILAT
                     PAGVAQEVADRLVNAGVRSILNFAPAVLNVPEGVSMRKVDLAIELQILSFYQQRRDEA
                     PALADERRN"
     gene            complement(1409..>2686)
                     /locus_tag="VFX21_11255"
     CDS             complement(1409..>2686)
                     /locus_tag="VFX21_11255"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5096586.1"
                     /translation="APSHELAREFEVVEPALVVASDEYADLVKRATARHRPPIPMLVL
                     DADVADAPEPIAPASRAADDLAVLLFTAGTAGPPKAAMLTHGSLLANLEQMQGHPGLR
                     IGADDVALAVLPWFHVFGLNVVLNLSLLAGASCSLVQHFHPAETIRRIGTDHVTVLAV
                     VPAVLAAWVAHDDAPADAFANVRLCVSGAAALPRETADAFERRFGLVVHQGYGLTEAS
                     PVVSTTAVAARPRPDSIGPPLPGVDVRLVDADGQDVLEGDAGEVLVRGANVFAGYWGD
                     EEATKQVLADGWLHTGDIAVADADGWLTLVERAKDVIIVSGFNVFPGEVEEAIGSHAD
                     VEDVAVIGEPHPRTGETVVAFVVPKPGADPDPVELLRHAGRRLARYKLPTRVDVVSEL
                     PRSFSGKLLRRELSSSGGGAAGGGDGNSPAVNR"
BASE COUNT          369 a          948 c          971 g          398 t
ORIGIN      
        1 cgttgcgccg ctcgtcggcg agcgcgggcg cctcgtcgcg tcgctgttgg tagaaggaga
       61 ggatctgcag ctcgatcgcg aggtcgacct tgcgcatcga cacgccctcg gggacgttca
      121 gcacggccgg ggcgaagttc aggatcgagc gcacgcccgc gttgacgagg cggtcggcga
      181 cctcctgggc gacgccggcc ggcgtggcca ggatgccgat cgcgatctgg cggtcggcga
      241 cgatctggtc gaggtcgtcc atcgcttcga tcgcgagctc gccgacctcg ccgccgacga
      301 ggtcggggtc ggcgtcgacg agggcgacga cccggaaccc gcgggcaccg aagccccggt
      361 agttcgcgag ggcccggccg aggtttccga tgccgacgat cacgaccggc cagtcgcggg
      421 tcaggcccag ctcccgggag atctcgtgga gcaggtactc gacgtcgtat ccgacgccgc
      481 gggtgccgta cgagccgagg tacgagaggt ctttgcggac cttggccgcg ttcacccccg
      541 ccagctccgc cagccgctcc gacgagatcg tcgccacctg gcgctcggcc gtctccagca
      601 gcgcccggta gtagagcggc agccgggcga cggtcgcttc ggggattcgg cgtgccccgc
      661 tcgacgggtc cgtgtcggcc acggagccca ccgtaccggc cttgtgagca aattctcaaa
      721 gtgtgcgagc tattttgagc agcgctgtgg tcgccctgct cgccttcatc gccacggcca
      781 tcgcgaccct gttcgctcag gcgaccgcgg tgcgctggtc tcgcacgcgt gcgccccacc
      841 agggcgcgtg gacgatcgcc ctcgcctgct tcgcgctcgc gtccgcggcg ctggcgaccg
      901 gcgcgtcgac ggggtgggac accggcacct tccgggcgtt ctacctcctc ggcgcgatcg
      961 tcaacgtccc gtggctcgcg ctgggcacga tctatctgct cggctcgcgg cgcgtcggca
     1021 acggcatccg cgtcgcgctg ttgctcttca ccgggctcgc aaccggcgtg atgttggcgt
     1081 gcaccgtgac acagccgatc gaccccgaca cgatccccgt cggcaaggag cacctccccg
     1141 tgttgccgcg cgtgctcgcc ggtgtgggca gcgggctcgg tgccgtcgtc gtgctcggcg
     1201 gcgcggtgtg gtcggcggtg cggttcgcgc gccagcgcgg tccgggtacg cgccggctcg
     1261 cgaccggcaa cacgttgatc gcgctgggca cgctgatcct gtcgagcggc ggactgctgc
     1321 aaggcgtcgt cggccacgac gaagcgttcg cgatctcgct cgcagccggc atcgcggtga
     1381 tctacgcggg attcgcggtc gcgtccggtc agcggttgac ggccgggctg ttcccgtcgc
     1441 cgccaccggc ggcaccgcct ccagacgacg acagctcgcg tcgcaagagc ttgccgctga
     1501 acgaccgcgg caactccgac acgacgtcga cgcgcgtcgg cagcttgtaa cgcgcgagcc
     1561 ggcgccccgc gtgccgcagc agctcgaccg gatccgggtc ggcgcccggc ttgggcacga
     1621 cgaacgcgac gacggtctcg cccgtacgtg gatgcggttc gccgatcacc gcgacgtctt
     1681 cgacgtcggc atgactgccg atcgcttctt cgacctcacc ggggaagacg ttgaagccgg
     1741 agacgatgat gacgtccttg gcccgttcga cgagcgtgag ccagccgtcg gcgtcggcga
     1801 ctgcgatgtc gccggtgtgc agccagccgt cggcgagcac ctgcttcgtc gcctcctcgt
     1861 cgccccagta gcccgcgaac acgttcgcgc cgcgcacgag gacctcgccc gcgtcgcctt
     1921 ccaacacgtc ttggccgtcg gcgtcgacga gacgcacgtc gacacccggc aacggcggcc
     1981 cgatcgagtc gggacgcggc cgggccgcga ccgcggtcgt cgagacaacc ggcgatgcct
     2041 cggtgaggcc gtagccctgg tgcacgacga ggccgaagcg gcgctcgaac gcgtccgcgg
     2101 tctcgcgcgg gagcgcggcc gcaccggaga cgcagaggcg cacgttcgcg aacgcgtcgg
     2161 ccggcgcgtc gtcgtgcgca acccacgccg cgagcacggc gggcacgacg gcgaggacgg
     2221 tgacgtggtc ggtgccgatg cgtctgatcg tctcggccgg atggaagtgc tgcacgagcg
     2281 agcacgacgc gcccgccaac aacgagaggt tgagcacgac gttcaacccg aacacgtgga
     2341 accacggcag caccgcgagc gcgacgtcgt cggcgccgat gcgcagaccc gggtggccct
     2401 gcatctgttc gaggttcgcg agcaacgaac cgtgcgtgag catcgcggcc ttgggtggtc
     2461 cggcggtgcc ggcggtgaac agcagcaccg cgaggtcgtc ggccgcgcgc gacgcgggcg
     2521 cgatcggttc gggcgcgtcg gcgacatccg cgtcgagcac gagcatcggg atgggtggtc
     2581 tatgacgcgc ggtcgcgcgc ttcacgagat ccgcgtactc gtcggatgcg acgacgagcg
     2641 cgggttcgac gacctcgaac tcgcgcgcga gctcgtgcga cggcgc
//
LOCUS       DASUGA010000570         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_112186, whole genome shotgun sequence.
ACCESSION   DASUGA010000570 DASUGA010000000
VERSION     DASUGA010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112186"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VFX21_11260"
     CDS             complement(<1..827)
                     /locus_tag="VFX21_11260"
                     /inference="COORDINATES: protein motif:HMM:NF017135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050080 - malonyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase family protein"
                     /protein_id="HEX5096587.1"
                     /translation="MAQRRFLDRVRRRGARRTPGRLELDVAPGLPERDVDRLREVVAD
                     VLRLHDTMTQRAEAAAVADAYLTLDDDGRRKFLHMLATEFWTDHTAVDDAIRALRQGD
                     DRRASERSLRDALAPPADSLLRLFTGLDGGVKFLVDLRGDVLRVGGRDEALADLSREL
                     KAHLATLFDVGLLALRRITWDAPATLLEKLIEYEAVHAIQSWDDLKNRLDSDRRCYAF
                     FHPAMPNEPLVFVEVALTTGIATTLQPLLDTRAANLDVDRADTAVFYSISNCQPGLAG
                     "
     gene            complement(837..>2033)
                     /locus_tag="VFX21_11265"
     CDS             complement(837..>2033)
                     /locus_tag="VFX21_11265"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5096588.1"
                     /translation="LLDASLAAGGMGFSSSWAKTHNDAAGDMVPSRYASEEELVELCG
                     VVRDHPGTTLEFIPCVGMFEDYAADLMTRMSLAANRPINWNVLFVSKGNNAMTDHNLA
                     ASDYAAERGAKVLALTMPAAPSPRICFDSGFFLDTIPGWEKPMALPHDEKLAMLSSPD
                     GRQTLRDLAAQPSTGFASTNWGIYIINETFAPENAQWEGKSVAEIAKARGVDEFDALL
                     DIVVADDLMKGFGFPMRPEDDDEWQARMKVWRDNRAVVGASDAGAHLDFLATFNYSTV
                     LLGGPVRARKLMPIEEAVRLLTDVPARLYGVKERGRLVEGWHADIVVIDPETVGAQPV
                     RMRFDLPTGAPRLFGGADGIDHVIVNGTEIVDHGEFTDARPGTLFRSGRDTETVSVPG
                     GNGTGT"
BASE COUNT          332 a          662 c          691 g          348 t
ORIGIN      
        1 ccggccagcc ccggctggca gttcgagatg gaatagaaca cggccgtgtc ggcgcggtcg
       61 acatcgagat tggcggctcg cgtgtcgagc agaggttgca gcgtcgtcgc gatgccggtg
      121 gtgagcgcga cctccacgaa cacgagcggc tcgttcggca tcgcgggatg gaaaaacgca
      181 taacagcgcc ggtcggagtc gagacggttc ttcaagtcgt cccacgactg gatcgcatgg
      241 accgcttcgt attcgatcag tttctcgagc aaagtcgcgg gcgcatccca ggtgatccgg
      301 cggagcgcga gcaagccgac atcgaacaag gttgccaagt gcgctttcag ctcgcgactc
      361 agatcggcga gcgcttcgtc gcgccccccg acgcgcaaca cgtcgcctcg tagatcgacg
      421 aggaacttca cgccgccgtc gagcccggtg aagagtcgca gcaggctgtc tgccggcggc
      481 gcgagcgcat cgcgcaaaga ccgttccgac gcgcgccggt cgtcgccttg gcgcaacgca
      541 cggatcgcgt cgtcgaccgc cgtgtgatcg gtccagaact cggtggcgag catgtgcagg
      601 aacttgcggc ggccgtcgtc gtcaagcgtg aggtacgcgt cggcgaccgc ggccgcctcg
      661 gcgcgttgcg tcatcgtgtc gtgcaggcgc agaacgtcgg cgacgacttc gcgtaggcgg
      721 tcgacgtcgc gttcgggcaa gccgggagca acgtcgagtt cgagtcggcc cggcgtacgc
      781 cgcgcgccgc gccggcggac ccggtcgagg aaccggcgtt gtgccatctg gtcaggctac
      841 gtgccggttc cgttcccgcc gggcacggaa accgtctccg tgtcgcggcc ggaacggaag
      901 agtgttccag gccgcgcgtc ggtgaattcg ccgtgatcga cgatttccgt cccgttcaca
      961 atcacatggt cgatgccgtc ggccccgccg aagaggcgcg gcgctccagt cggcaggtcg
     1021 aatcgcatcc gaaccggttg cgcaccgacc gtctcgggat cgatgacgac gatgtcggcg
     1081 tgccagccct cgacgaggcg accgcgctcc ttgacgccat aaagccgcgc cggcacgtcg
     1141 gtgagcaggc gcactgcttc ctcgatcggc atgagcttgc gcgcccgcac cggtccgccg
     1201 agcaacaccg tcgaatagtt gaaggtcgcg aggaagtcga ggtgggcacc tgcgtcgctc
     1261 gcaccaacga ccgcacgatt gtcgcgccac accttcatgc gcgcttgcca ctcgtcgtcg
     1321 tcttcgggtc gcatcgggaa accaaagccc ttcatgaggt cgtcggcgac gacgatgtcg
     1381 agcaacgcgt cgaactcgtc gaccccgcgc gccttggcga tctcggcgac gctcttgcct
     1441 tcccactgcg cgttctcggg cgcgaacgtc tcgttgatga tgtagatccc ccagttcgtg
     1501 ctcgcgaacc cggtcgacgg ctgcgcggcc agatcacgca gcgtttgccg tccgtcgggt
     1561 gatgagagca tcgcgagttt ctcgtcgtgg gggagcgcca tcggcttttc ccaaccggga
     1621 atcgtgtcga ggaagaagcc gctgtcgaag cagatccgcg gtgacggtgc ggcgggcatc
     1681 gtgagcgcga ggaccttggc gccgcgctcg gctgcgtagt cggatgccgc caggttgtgg
     1741 tcggtcatcg cgttgttgcc cttcgacacg aacaacacgt tccagttgat cgggcggttg
     1801 gccgcgagcg acatgcgcgt catcaggtcg gcggcgtagt cctcgaacat gccgacgcac
     1861 ggaatgaatt cgagggtcgt gccgggatgg tcgcgcacca cgccgcacag ctcgacgagt
     1921 tcttcttcgc tcgcataacg cgagggcacc atgtcgcccg ccgcgtcgtt gtgggtcttc
     1981 gcccacgagg acgagaatcc catgccgccc gcggcgaggc tcgcgtcgag gag
//
LOCUS       DASUGA010000571         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1856190, whole genome shotgun sequence.
ACCESSION   DASUGA010000571 DASUGA010000000
VERSION     DASUGA010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1856190"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VFX21_11270"
     CDS             complement(<1..627)
                     /locus_tag="VFX21_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5096589.1"
                     /translation="MTSAAITYDPFAPDVQADPYPFYAYLRRDAPVYYVESLDAYAIS
                     RHADVRRVMREHATFTSEAMAELVTRPVNIGGGEGFEASIDESISIVGRDGEDHTRLR
                     TIVNRGFTPRRIAQLEEEICTIAQPFLAELVSKRDGDLMADFAVPFPTSVIAAMLGVD
                     PARRDDFRRWSEFMVLAVFEPMTPEQMAGVAQSSDEMGEYLDGVFEERR"
     gene            complement(624..1238)
                     /locus_tag="VFX21_11275"
     CDS             complement(624..1238)
                     /locus_tag="VFX21_11275"
                     /inference="COORDINATES: protein motif:HMM:NF019960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5096590.1"
                     /translation="MPGRTPQQVQVLDAAVRLFADRGFDDVTMADIADAAGVARATVF
                     NYFGSKHGVVEAVTESVIVYYRELLDQALADDETPAPVLLRWLFEEMGTGIETQRRFF
                     RGVFREIARIGLGFDEGGVTQRAEEDASTRLEQLIERGQARGELNDTFEAKVLALAFR
                     ALANGTINNWLYDDASEPLTVRMGAVVDIFLSPIAVPTMERGAR"
     gene            complement(1255..1566)
                     /locus_tag="VFX21_11280"
     CDS             complement(1255..1566)
                     /locus_tag="VFX21_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5096591.1"
                     /translation="MRLQRGAVVAVVVGGAEYVVWRGHSGCLGSAPRSCPHLDTDLAD
                     ARVVGDELVCPTHGWSFDCAGRAFKRNEFGREDDKGTVGRLGLTEAANGEIEVLDSGP
                     T"
     gene            complement(1563..1823)
                     /locus_tag="VFX21_11285"
     CDS             complement(1563..1823)
                     /locus_tag="VFX21_11285"
                     /inference="COORDINATES: protein motif:HMM:NF040429.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6457 domain-containing protein"
                     /protein_id="HEX5096592.1"
                     /translation="MLEWVMDWITDATLHLAAATGLDPALLMVDAIATEQLLDLAGIA
                     AHSSGARTNAPLLCHVIGRAVALGASLDIVDAAVREFAGSGD"
     gene            1946..>2301
                     /locus_tag="VFX21_11290"
     CDS             1946..>2301
                     /locus_tag="VFX21_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096593.1"
                     /translation="MTSILAALPVAGGALQTGPSPGATLIAYVGVAAGTSSAYTSAGP
                     GAPWTAAPSRDLPDVGSNAWPVLGDLDGDGDSDAMVGDAAGFMHAFRNNGTDQNPSWA
                     AMTAWDPSFDAGSNAAP"
BASE COUNT          359 a          783 c          797 g          362 t
ORIGIN      
        1 ccggcgttcc tcgaagacgc cgtcgaggta ttcgcccatc tcgtccgagc tctgtgcaac
       61 gcccgccatc tgttcgggcg tcatcggttc gaacactgcg agcaccatga actccgacca
      121 gcgccggaag tcgtcgcgcc gcgcggggtc gacgccgagc atggccgcga taacgctcgt
      181 cgggaacggc accgcgaagt cggccatgag gtcgccgtcg cgcttgctca cgagctccgc
      241 aaggaagggc tgcgcgatcg tgcaaatctc ctcctcgagc tgcgcgatgc ggcgcggcgt
      301 gaagccgcgg ttcacgatcg tccgaagacg cgtgtggtcc tcgccgtcac ggccgacgat
      361 cgagatcgac tcgtcgatcg atgcctcgaa accctccccg ccgccgatgt tgacgggccg
      421 cgtgacgagc tccgccatcg cctcggaggt gaacgtggcg tgctcgcgca tgacccgtcg
      481 cacgtcggcg tggcggctga tcgcgtacgc atcgagcgat tcgacgtagt agacgggcgc
      541 gtcgcggcgc agatacgcat agaacgggta cgggtcggcc tgtacgtcgg gtgcgaacgg
      601 gtcgtaggtg atcgcggccg acgtcatcgt gcacctcgct ccatggtggg gacggcgatc
      661 ggcgagagga agatgtcgac gaccgcaccc atccggacgg tgagcggctc cgacgcgtcg
      721 tcgtagagcc agttgttgat cgtcccgttg gcgagcgcgc ggaacgccaa cgcgagcacc
      781 ttcgcctcga acgtgtcgtt cagctctccg cgcgcttgcc cacgctcgat gagttgctcg
      841 agacgcgtgc tcgcatcttc ttcggcgcgc tgcgtgacgc cgccttcgtc gaagccgagg
      901 ccgatgcgcg cgatctcgcg gaacacgccg cggaagaagc gccgttgggt ctcgatgccg
      961 gttcccatct cctcgaagag ccatcgcagc aggacgggcg ccggggtttc gtcgtcggcg
     1021 agcgcctggt cgagcagctc gcggtagtag acgatgacgg actcggtgac ggcttcgacg
     1081 acgccgtgct tggaaccgaa gtagttgaag accgtcgccc gcgcgacgcc ggcggcgtcc
     1141 gcgatgtcgg ccatcgtcac gtcgtcgaac cctcggtcgg cgaacagccg gaccgcggca
     1201 tcgaggacct gcacctgctg tggtgtacgc ccgggcattg gaccggagtc taaatcaggt
     1261 tggacccgag tccaagacct caatttcgcc gttcgcggct tccgtcaacc cgagccgtcc
     1321 gaccgtgccc ttgtcgtcct cgcggccgaa ctcgttgcgc ttgaacgctc ggcccgcgca
     1381 gtcgaacgac caaccgtggg tcgggcagac gagctcgtcg ccgacgactc gcgcgtccgc
     1441 gagatcggtg tcgaggtgcg ggcagctgcg cggcgcgctg cccagacatc cgctgtgccc
     1501 gcgccacacg acgtactccg caccgccgac gacgacggcg acaactgcgc cgcgttggag
     1561 tctcaatctc cggacccagc gaactcgcgt acggcggcgt ccacgatgtc gagcgaggcg
     1621 ccgagggcga ccgcgcgccc gatgacgtga cacagcagcg gtgcgttcgt gcgggcgcca
     1681 ctgctgtgcg ccgcgatgcc ggcgaggtcg agcagctgct cggtcgcgat cgcgtcgacc
     1741 atcaacaacg cgggatcgag gccggtggcc gcggcgagat gcagggtcgc atcggtgatc
     1801 cagtccatga cccattcaag catcgagacg tttgtctccg ataactgctg cgaggcgggc
     1861 tgtgaggctc tgttggggga tcctcgtgcc agccgttcgc gcctacgtac caaatcgcca
     1921 ccgtcgccgg tggggagccg tcaccgtgac gagcatcctc gccgcactgc cagtggcggg
     1981 cggcgcgttg cagacgggcc cgtcgcccgg cgcaacgctc atcgcgtacg tgggtgtggc
     2041 ggccggcacg agcagcgcgt acacgtcggc cggtccgggg gcgccgtgga ccgcggcgcc
     2101 gagccgcgat cttcccgacg tcggttcgaa cgcgtggccg gtgctcggcg acctcgacgg
     2161 cgacggcgac agcgacgcga tggtcggcga cgccgcaggc ttcatgcacg cgttccgcaa
     2221 caacggcacc gaccagaacc cgtcgtgggc cgcgatgacc gcgtgggacc cgagcttcga
     2281 cgcagggtcg aacgcggcac c
//
LOCUS       DASUGA010000572         2474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1447760, whole genome shotgun sequence.
ACCESSION   DASUGA010000572 DASUGA010000000
VERSION     DASUGA010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447760"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /gene="ligD"
                     /locus_tag="VFX21_11295"
     CDS             <1..1011
                     /gene="ligD"
                     /locus_tag="VFX21_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX5096594.1"
                     /translation="WMIHRMDPPADPGRAAPPARIAPMQATLAKRAPRDEGWAWELKW
                     DGIRALGYADGGRLRLFTRNGNEVTKRWPELRRLAVELGARDAVFDGEIVTFDEHGRP
                     SFETLQRRMHVDSETQIRRLQHEVPAVYILFDLLWLDGHSLMELPYEERRAQLLDLDL
                     TGASWQTPPNEVGDGAATIEVSRRFGLEGVVAKRLGSIYEPGKRSKAWIKVKNSRRQE
                     FVVGGWMPGEGSRDGSIGSLVVGYYEDVDGEPVLHYAGRVGSGLSHADITELERCFAK
                     YPRETSPFATGSPPRQARWVEPMLVVEVRFTEWTKAGTLRQPSFLGYRTDKSPLDVVR
                     ES"
     gene            complement(982..1731)
                     /locus_tag="VFX21_11300"
     CDS             complement(982..1731)
                     /locus_tag="VFX21_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019045946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX5096595.1"
                     /translation="MLRVGTSGWQYRDWRGRFYPKALPQKRWLEHYAEHFGTVEINNT
                     FYRLPARATFEQWAERVPDGFVVAVKASGYLTHYRRLREPEEPVARLMDRAVALGDHL
                     GPILLQLPPDMKAEPGRLDATLRAFNRRARIAVEPRHESWFDDEVRAVLVEHGAALCL
                     ADRKSRPVTPLWRTADWCYLRLHQGAARPAPCYGEHALASWSARLRDLWGARVDGYVY
                     FNNDTHGCAVRDAETFARIAARSGFAHDVQG"
     gene            1783..>2474
                     /locus_tag="VFX21_11305"
     CDS             1783..>2474
                     /locus_tag="VFX21_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme"
                     /protein_id="HEX5096596.1"
                     /translation="MSRRRAPSRPPTAPRAQDAAPRIANLAVWPGTPFPLGATWDGSG
                     TNFSCFSESAEHIDLCLFDDAGHETRVPMREVSAFVHHVSLPGIGPGQRYGFRAHGLW
                     SPAQGKRANPHKLLLDPYGKAVTGSVNWGQPVYPYRFGHENRISTSDSARFMPKSVVA
                     NPYFDWGNDRPPNTPLHETVVYELHVKGFTQLHPAVPPNLRGTYLGLATPEVVDWLLG
                     LGVTAVELMPVH"
BASE COUNT          409 a          861 c          837 g          367 t
ORIGIN      
        1 tggatgatcc accggatgga cccgccggcc gatccgggcc gcgccgcgcc gcccgcgcgc
       61 attgcgccga tgcaggccac gctcgccaag cgcgcgcccc gcgacgaggg ctgggcgtgg
      121 gagctgaagt gggacggcat ccgcgcgctc ggttacgcgg acggcgggcg cctccgactg
      181 ttcacccgca acggcaacga agtcacgaaa cgatggcccg agttgcgccg gctcgcggtc
      241 gagctcggag cgcgtgacgc ggtgttcgac ggtgagatcg tcacgttcga cgaacacggt
      301 cgcccgagct tcgagacgtt gcagcggcgc atgcacgtcg actcggagac gcagatccgg
      361 cgcctccaac acgaagtccc cgcggtgtac atcttgttcg acctcctgtg gctcgacggg
      421 cactcgttga tggagctgcc gtacgaggag cggcgtgcgc aactgctcga cctcgacctg
      481 accggcgcgt cgtggcagac accaccgaac gaagtcggcg acggcgcggc gacgatcgag
      541 gtgagccggc gcttcggcct cgaaggtgtc gtggcgaagc gcctgggcag catctacgag
      601 cccggcaagc gctcgaaggc gtggatcaag gtgaagaact cgcgccggca ggagttcgtc
      661 gtcggcggct ggatgccggg cgaaggaagc cgcgacgggt cgatcgggtc gttggtcgtc
      721 ggttactacg aagatgtcga cggcgagccg gtcctgcact acgcggggcg cgtcggcagc
      781 gggctcagcc acgccgacat caccgagctg gagcgctgct tcgcgaagta cccgcgcgag
      841 acgagcccct tcgcaacggg cagtccgcca cggcaggcgc gttgggtcga accgatgttg
      901 gtcgtcgaag tccgcttcac cgagtggacc aaggccggca cactgcggca gccgtcgttc
      961 ctcggatacc ggacggacaa gtcacccctg gacgtcgtgc gcgaatcctg agcgcgccgc
     1021 gatacgggcg aaggtctcgg cgtcgcgcac cgcacacccg tgggtgtcgt tgttgaagta
     1081 gacgtatccg tcgacgcgcg ccccccacag gtcgcgcagg cgcgccgacc aggacgcgag
     1141 cgcgtgttcg ccgtagcacg gcgcgggtcg cgccgcgccc tgatgcaggc gcaggtagca
     1201 ccagtcggcc gtgcgccaca acggtgtcac cggtcgcgac ttgcggtccg cgaggcacag
     1261 cgcggcgccg tgctccacca acacggcgcg gacctcgtcg tcgaaccacg actcgtgacg
     1321 cggctcgacc gcgatccggg cccgccggtt gaacgcgcgc aaggtcgcgt ccaaccgtcc
     1381 gggctccgcc ttcatgtccg gcgggagttg cagcaggatc ggtccgagat gatcaccgag
     1441 cgcaaccgcg cgatccatga gccgcgcgac cggctcttcg ggctcccgca ggcggcgata
     1501 gtgcgtgaga tacccgctcg ccttgaccgc gacgacgaac ccgtccggga ctcgttcggc
     1561 ccattgctcg aacgtcgcgc gcgcaggcag acggtagaag gtgttgttga tctccaccgt
     1621 gccgaagtgt tcggcgtagt gctcgagcca acgcttctgc ggcagcgcct tgggatagaa
     1681 gcgaccgcgc cagtcgcgat actgccaacc ggacgttcca acacgaagca cgcgagcgta
     1741 gctacccgtc cgctgcaaat gggtaacttc gacactccga acatgtcccg tcgccgagcg
     1801 ccctcgcgcc ctcccactgc accacgcgcc caggacgcgg cgccgcgcat cgcgaacctc
     1861 gcggtctggc ccggcacacc gttcccgctc ggtgcgacat gggacggttc cggcaccaac
     1921 ttctcgtgct tctcggagtc ggcagagcac atcgacctgt gcctgttcga cgacgcggga
     1981 cacgaaaccc gcgttccgat gcgcgaggtc agcgcgttcg tgcaccacgt ctcactgccc
     2041 ggcatcggtc cgggccagcg ctacggcttt cgcgcgcacg gcctctggtc acccgcgcag
     2101 ggaaagcgcg ccaaccccca caagctgctg ctcgatccgt acggcaaggc cgtcaccggt
     2161 tcggtgaact ggggtcagcc cgtgtatccg taccggttcg gtcacgagaa ccggatcagc
     2221 acgagcgaca gtgcgcggtt catgccgaag tcggtcgtcg ccaatccgta cttcgactgg
     2281 ggtaacgacc ggccgccgaa cacgccgttg cacgagacgg tcgtgtacga gctccatgtc
     2341 aaggggttca cgcaactgca cccggccgtg ccaccgaacc tgcgcggcac gtacctgggc
     2401 ctcgcgaccc ccgaggtcgt cgactggctg ctcggcctgg gcgtgaccgc ggtcgaactg
     2461 atgcccgtgc acca
//
LOCUS       DASUGA010000573         3013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1050479, whole genome shotgun sequence.
ACCESSION   DASUGA010000573 DASUGA010000000
VERSION     DASUGA010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050479"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="VFX21_11310"
     CDS             <1..150
                     /locus_tag="VFX21_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5096597.1"
                     /translation="EELLAELETKEAFGRAATPEEVANVIIFLASDLASYMTGEVLSV
                     SSQHP"
     gene            260..1198
                     /locus_tag="VFX21_11315"
     CDS             260..1198
                     /locus_tag="VFX21_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096598.1"
                     /translation="MKQRLCLLQGIARTQFGLFTYAQAISVDIPPRTIAWMLDRGRWQ
                     EVEPGVMRALPAGRLTWQERIFALSLSVDGVGFGLSASALYGVWTPPRQAEVLVERAG
                     RNRKRPGVHSTRSLPKSDIAIVQGVPATMPARSLCDGAAGLRHEHVNKLVDTAVARGL
                     VQPSVLAKRAFELRNSKRPGCRKVLLALAEQHPGLEQARNEWEALVVRRVHEFGLPEP
                     QLDYPVVVDGQQRYLDVAWAQSLVDLEFDGFLPHMVRSVFDEDRVRQNSLVAAGWTVF
                     RVTSRILEQNPGPLFGQIRQALRSRGHHFGKMLPVS"
     gene            1279..2019
                     /locus_tag="VFX21_11320"
     CDS             1279..2019
                     /locus_tag="VFX21_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096599.1"
                     /translation="MKLSGIIAICTTSAVLATAGVSVAGAASSTGTTEPTAATANAHR
                     PAARRAIAGALKVAADAIGITRAQLATELKAGKSVAEVATAHDKTPQSIVDALVAAGT
                     KRINAAKDAGKITDEQAQTALEKLPARAEQAVNAKAGDRKARKHHARVRRLAGGALRR
                     AADVIGIEPKALIAELRSGKTIAEVATAKGKAPQAVIDAIVAAGSERINTAHDNHKLT
                     DEQAAALLDKLPGRAEKFVNEWTPKSGK"
     gene            2089..2967
                     /locus_tag="VFX21_11325"
     CDS             2089..2967
                     /locus_tag="VFX21_11325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5096600.1"
                     /translation="MVRLPLLDRAGESALARLMERGRQAEARLSVDALPPDERHELAQ
                     HVSEGLRARRRFIEANTRLVVSVARTFQGRGLSLDDLIQEGNAGLVRAVDKFDAHRGF
                     KFSTYAVWWIRQGIQRALSEQGHSLRLSSSGADDLARVQRMISDFEDEHRREPTLGEI
                     AVHTGLPVERIRRVLDAIAPVVSLDAPLHFADGTSGDTLGSLVADAAPPVDDLAIHST
                     YRGVLDRMMGVLDERERVRVCMRFGLDGEDPAQSAAIAKVLHLSTERVRQIEARALSK
                     LRHPSVEPEARLLLAG"
BASE COUNT          522 a         1044 c          972 g          475 t
ORIGIN      
        1 gaggaattgc tggccgagct cgaaaccaag gaagccttcg gccgggccgc aactcccgaa
       61 gaggtcgcca acgtcatcat cttcttggcc agcgacctcg ccagctacat gacgggcgaa
      121 gtcctttccg tcagcagcca acacccctag cccgtggtca ccgattcggc aacatgttgc
      181 cgaatcggtg accacgcagc agttgtctca cccccgtgtg acgatgcggc gacttcccca
      241 tccacacggg gaggtatcga tgaagcaacg gctctgcttg ctccaaggca ttgcgcgaac
      301 ccagttcggg ctcttcacct acgcgcaggc gatctcggtc gacatcccgc cgcgcacgat
      361 cgcgtggatg ctcgaccgcg gtcgctggca ggaggtcgag cccggcgtga tgcgtgcgct
      421 tccggccggt cggctcacat ggcaggaacg catctttgcc ctgagcctct ccgtcgacgg
      481 cgtcggattc ggactcagcg cctccgcgct ctacggcgtc tggacaccgc cacgccaagc
      541 agaggtgctc gtcgagcgcg ccggtcgaaa tcgcaagcga ccgggcgtgc acagcacgcg
      601 ctctttgccc aagtcggata tcgcgatcgt ccagggtgtt ccagcgacca tgcctgcgcg
      661 ctcgctctgc gacggagccg caggcttgcg gcatgagcac gtgaacaagc tcgtcgacac
      721 ggccgtcgcc cgcggcctcg ttcaaccctc cgtcctcgcc aaacgcgcgt ttgaactgag
      781 gaattcaaaa cgtccaggct gtcgcaaggt attgctcgcg ctcgccgagc agcatccggg
      841 tctcgaacag gcgcggaacg agtgggaagc actcgtcgtg cgacgcgtgc acgagtttgg
      901 actcccggag ccacaactcg actacccggt tgtcgtcgac gggcaacagc gatacctgga
      961 cgttgcctgg gcccaatctc tcgtcgacct ggaattcgat gggttccttc cgcacatggt
     1021 ccgttccgtc tttgacgagg atcgggtgcg acagaactcg ctggtggcgg cagggtggac
     1081 cgtctttcgg gtgacctcgc gaatcctcga gcagaatccg ggaccgcttt ttggacagat
     1141 ccgtcaggct ctgcgatccc gtggtcacca tttcggcaag atgttgccgg tttcgtgacc
     1201 acggggcccg gggcagccat cgcccgcccc tcgcgccggc ttcacactgc cctcacattc
     1261 ggggccgagg atgcggatat gaagctctcc gggatcattg ccatctgcac aacctcggcc
     1321 gtgctcgcga cggcgggcgt ctcggtcgcc ggcgccgcat cctcgaccgg cacgaccgag
     1381 ccgacggccg ccactgcgaa cgcgcaccgg cccgccgccc gccgcgctat cgccggcgcc
     1441 ctgaaggtcg cggccgacgc gatcggcatc acgcgggcgc aacttgccac cgagttgaag
     1501 gccgggaaga gcgtcgccga agtggcgacc gcccacgaca agacgccgca gtccatcgtt
     1561 gacgcgctcg tcgccgcggg cacgaagcgc atcaacgcgg cgaaggacgc gggcaagatc
     1621 accgacgagc aggcgcagac tgcgctcgaa aagcttccgg ctcgggccga gcaggccgtc
     1681 aacgccaagg ctggtgaccg caaggcgcgc aagcaccacg cccgcgtgcg ccggctcgcg
     1741 ggcggcgcac tccgtcgcgc ggccgacgtg atcggtatcg agcccaaggc actgatcgca
     1801 gagctgcgga gcggcaagac catcgccgag gttgcgaccg cgaagggcaa ggcgccgcag
     1861 gcggtcatcg acgcgatcgt ggcggccggt agcgagcgga tcaacaccgc ccacgacaac
     1921 cacaagctca ccgacgaaca agccgccgcg ctgctcgaca agcttcccgg ccgggccgag
     1981 aaattcgtca acgaatggac gcccaagtcc ggcaagtgac ccatccggtt ctaccctcgg
     2041 tgccgaacag gagttgaacg ctcccggacg tcatcggagg tggacggcat ggttcgtctt
     2101 ccgctgctcg accgggccgg cgaatcggcg ctggcccggc tcatggaacg aggccgccag
     2161 gctgaggctc gtctctcggt cgacgccctg ccgcccgacg aacgtcacga actcgcgcag
     2221 cacgtttccg aagggctgcg ggcccggcgc cggttcatcg aagccaacac ccggctcgtc
     2281 gtttcggtcg cacgaacgtt tcaaggccga ggcctgtcgc tcgacgacct catccaggag
     2341 ggcaacgccg gactggtacg ggcggtcgac aagttcgatg cgcaccgcgg cttcaagttc
     2401 tcgacgtacg cagtgtggtg gatccggcaa ggcattcagc gtgccctgtc ggaacagggt
     2461 cattcgttgc ggctttcgtc gagcggcgcc gacgatctcg cacgcgtgca acgcatgatc
     2521 tccgacttcg aagacgagca tcggcgggag cccaccctcg gcgagatcgc agttcacacg
     2581 ggcttgccag tggaacgtat tcgtcgagtg ctcgacgcca tcgcgccggt ggtgtcgctt
     2641 gatgcgccgc tgcactttgc cgacggcacg agcggcgaca cgctcggctc ccttgtcgcc
     2701 gacgcggcgc cgcccgtcga cgatctcgcc atccactcga cgtaccgcgg cgtgctcgac
     2761 cgcatgatgg gcgtgctcga tgaacgcgag cgagtccgcg tgtgcatgcg cttcggactc
     2821 gacggcgaag acccggctca aagcgctgcg atcgcgaagg tgctccacct ctcgaccgaa
     2881 cgggtgcggc agatcgaagc gcgagcgctc tcgaaactgc ggcacccgtc ggtcgagccc
     2941 gaggcgaggc tgcttctcgc cggttagcgc tcggcccggc gccggcccgc cagcaacgcg
     3001 gcgccggtcg cga
//
LOCUS       DASUGA010000574         3150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1076736, whole genome shotgun sequence.
ACCESSION   DASUGA010000574 DASUGA010000000
VERSION     DASUGA010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076736"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..2759)
                     /locus_tag="VFX21_11330"
     CDS             complement(45..2759)
                     /locus_tag="VFX21_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEX5096601.1"
                     /translation="MSVTAGQSPTAASEAVGWHGLSAQEVADKLGTNLDTGLSASDAA
                     ERLQRYGPNALQKEVPPSRWVIMGQQVRDPMNIMLLAVAIVSVLIREHSTAVLVGLLV
                     WVNVAMGTRQELKARASVEALAEMQIPQARVMRDGQLTAVPATDVVPGDLVAVESGDI
                     VVADGRIVRATNMETQEAALTGESAPIPKGAETVAADAALGDRIDMTFQNTSVTRGTA
                     NFVVTATGMETEVGKIAGLLQSVQRTRSPLQQQLDGLTKWLGVVAWTALAIVVVAGMI
                     RGLEFSDLLLLGTAMAISAIPTGMPTFVQGMLSYGARKLAEARAIVRNLSDVETLGAT
                     SAINTDKTGTLTLNQMTATKLFYSGEWFNIEGGGYSKQGAILGSAGQDIPDFQPLALG
                     CVLASDATVTDEGEAIGDPTEAALIVLAAKVGVDAGITRRQYPRLADVPFDSEYKYMA
                     TFHRHTRDGEERIVEIVKGAPDVLLDLSTHAYWNGEQVDVATVRDDILRANRELSEAG
                     LRVLALGFRRMTPDQAPAIEKDPQGEVRDLVFGALVGIIDPLRTEAKDAVKIALAAGI
                     DVRMITGDHTITARAIADQLGLGEGVLTGPEFAALSDDEIKDRLPQLHVFGRVSPEDK
                     LRLASLMQEQGLVVAMTGDAVNDAAALKKADIGVAMGSGSEVSKQAAKMVLTDDNFAT
                     LVHAVELGRDIYHKVTAYIRYQMSQLFGLVSLFLIAAVFGINDGVALQPMQAIFLNFF
                     VAIFPVIAIMTDVTDPTVMRDKPRDPKVAIFNKTTGPRWILYGLVLGVMSMIPLVWGP
                     DEPSIDNASVAMTMAFAVMGASTILSGFVMRHDREMAFANMTLKFAGTLGIGALVVIF
                     ATEFRFLQQWLLTTSLTGPQWAAVLGLALVMPIVVGADKTIQRMRARR"
     gene            2848..>3150
                     /locus_tag="VFX21_11335"
     CDS             2848..>3150
                     /locus_tag="VFX21_11335"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005692790.1"
                     /note="reversible synthesis of carbamate and ATP from
                     carbamoyl phosphate and ADP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEX5096602.1"
                     /translation="MARVVVALGGNALLRRGQPMSVANQIENVRTACDRLAPIAEQHE
                     LVVSHGNGPQIGLLALEEAAYEDTPDAPLDVLGAETQGMIGYLIEQELGNRLPFDKP"
BASE COUNT          526 a         1067 c         1021 g          536 t
ORIGIN      
        1 gcctgcgagt cgagatccga catcagcccc ccaatgcgag tagctcaacg gcgagcgcgc
       61 attcgttgga tcgtcttgtc ggcgccgacg acgatcggca tcacgagcgc gagcccgagc
      121 accgcggccc actgcggacc ggtgagcgat gtcgtcagca gccattgctg caggaagcgg
      181 aactcggtgg cgaagatgac caccaatgca ccgatgccga gcgtgcccgc gaacttcagc
      241 gtcatgttcg cgaacgccat ctcacgatcg tgccgcatca cgaatcccga caggatcgtg
      301 ctcgcgccca tcactgcgaa cgccatggtc atcgccaccg atgcgttatc gatgctcggc
      361 tcgtcggggc cccacacgag cgggatcatc gacatgacac cgagcacgag gccgtagagg
      421 atccaccgcg gcccggtggt cttgttgaag atcgcgacct tcggatcgcg tggtttgtcc
      481 ctcatgaccg tgggatcggt cacgtcggtc atgatcgcga tcacggggaa gatggcgacg
      541 aagaagttga ggaagattgc ctgcatcggt tgcagcgcga caccgtcgtt gatgccgaac
      601 acggcggcga tgaggaacaa cgacacgagg ccgaacagct ggctcatctg gtagcggatg
      661 tacgccgtga ctttgtggta gatgtcgcgg ccgagctcga ccgcgtgcac gagcgtcgca
      721 aagttgtcgt cggtgagcac catctttgcg gcctgcttcg acacctcgct gccgctgccc
      781 atcgcgacgc cgatgtcggc cttcttcaat gcggccgcgt cgttcaccgc gtcgccggtc
      841 atcgcgacga cgagcccttg ctcttgcatc agtgacgcga ggcgcagctt gtcctcgggc
      901 gacacgcgac cgaacacgtg cagctgcggc aatcggtcct tgatctcgtc gtcggagagc
      961 gcagcgaact ccggaccggt cagcactccc tcaccgaggc ccaactggtc cgcgatggcg
     1021 cgggcggtga tggtgtggtc gccggtgatc atgcgcacgt cgatgccggc cgcgagtgcg
     1081 atcttcaccg cgtccttcgc ctcggtgcgc aacgggtcga tgatgccgac cagcgcgccg
     1141 aacacgaggt cgcgcacttc gccctgcggg tccttttcga tcgccggcgc ctggtcgggc
     1201 gtcatgcgcc ggaatccgag cgccaacacg cgcagccccg cctccgacag ctcccggttg
     1261 gcgcggagaa tgtcgtcgcg cacagtcgca acgtcgactt gctcaccgtt ccagtacgcg
     1321 tgcgtgctca ggtcgaggag cacgtcgggc gcgcccttca cgatctcgac gatccgctcc
     1381 tctccgtcgc gcgtgtggcg atggaacgtc gccatgtact tgtactcgga gtcgaacggg
     1441 acgtcggcga gacgcgggta ctggcgacgc gtgataccgg cgtcgacacc gaccttcgcc
     1501 gcgagaacga tcagcgcagc ttcggtcggg tcgccgatcg cctcgccttc gtcggtgacc
     1561 gtcgcgtcgc tcgcgagcac gcacccgagc gcgagcggct ggaagtcggg gatgtcctgg
     1621 cccgcgctgc cgaggatcgc gccctgcttc gaatagccac caccttcgat gttgaaccac
     1681 tcgcccgagt agaacagctt cgtcgccgtc atctggttga gcgtcagggt gccggtcttg
     1741 tcggtgttga tcgcgctcgt cgcaccgagc gtctcgacgt cactcagatt gcggacgatc
     1801 gcccgcgctt cggcaagctt gcgcgcgccg tacgagagca ttccctgcac gaacgtcggc
     1861 atgccggtcg gtatcgcgga gatggccatc gcggtaccga ggagcaacag atcgctgaac
     1921 tccaaaccgc ggatcattcc ggccacgacg acgatcgcga gtgccgtcca cgcgacgaca
     1981 ccgagccact tcgtgaggcc gtccagttgc tgctgcaacg gagaacgcgt gcgttgcacc
     2041 gactgcaaca agcccgcaat cttgccgacc tcggtctcca tccccgttgc agtgacgacg
     2101 aagttcgcgg tgccgcgagt gaccgaagtg ttctggaacg tcatgtcgat gcggtcaccg
     2161 agggcggcgt cggcggcgac ggtctccgcg cccttgggga tcggtgcgct ctcaccggtg
     2221 agcgcagcct cctgcgtctc catgttggtc gcgcgcacga tgcgaccgtc ggccacgacg
     2281 atgtcaccgg actcgaccgc gacgagatcg cccggcacca cgtcggtcgc cgggacggcc
     2341 gtgagctggc cgtcccgcat cacgcgggcc tgcggaatct gcatctccgc cagggcttcc
     2401 acgctcgccc gcgccttcag ctcctgccgc gtgcccatcg cgacgttcac ccacacgagc
     2461 aacccgacga gcaccgcggt cgagtgctcg cgaatcaaca cgctgacgat cgccaccgcc
     2521 agcaacatga tgttcatggg gtcgcgcacc tgctgaccca tgatcaccca gcgcgagggc
     2581 ggaacttcct tctggagcgc gttcggcccg taacgctgca gccgctcggc cgcgtcgctc
     2641 gcgctcaggc cggtgtcgag attggtgccg agcttgtcgg cgacctcttg cgcgctgagc
     2701 ccgtgccacc cgacggcctc ggacgcggcg gtgggcgact gacccgcagt gacgctcatg
     2761 acggtgagcg tacggcagac tcgatccccg gagccggagg cttaagcctc tagcttgcgg
     2821 tcgtctcccg gtagcaggat cctgaccatg gcgagagtcg tcgtcgcact cggtggcaac
     2881 gcgctgttgc gccgcggcca accgatgagc gtcgcgaacc agatcgagaa cgtccgtacc
     2941 gcgtgcgacc ggctcgcgcc gattgccgag cagcacgagc tcgtcgtgtc ccatggcaac
     3001 ggcccgcaga tcgggttgct cgcgctggaa gaggcggcgt acgaggacac gccggacgcg
     3061 ccgctcgacg tgctcggcgc ggagacgcaa gggatgatcg gctacttgat cgaacaggag
     3121 ttggggaacc gcctgccgtt cgacaagccc
//
LOCUS       DASUGA010000575         4694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1328342, whole genome shotgun sequence.
ACCESSION   DASUGA010000575 DASUGA010000000
VERSION     DASUGA010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328342"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1764
                     /locus_tag="VFX21_11340"
     CDS             169..1764
                     /locus_tag="VFX21_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5096603.1"
                     /translation="MTATASPFTGLAPLSRFTIRRDRVRIVVWIAAIVLLVVVTVASV
                     KGLYPNQEELDKAARASEDNVAAIIFNGPPQALDTVGGQVAFQTGTWGLILIGMMSIF
                     MLGRLTRGEEEAGRNELLRSLPIGAHSLPGAALLTVGAMNVVTGALVTIVLLALSLPA
                     TGSVVFGLSFTMFGLLLAAVTLVAAQITENTRVVYGLGGVVLGASFVLRAIGDIGDGT
                     VSWLSPIGWAQKTRPYAGENWWPFLLIAVATGALAWVAGVLSRHRDLGSGLIAPRTGR
                     ARAAPSLGTPLGLATRVQRGSLLGWSAGLLFLAVAYGSITDSIDQFVEDNDALNDIIA
                     AQGNGTLVEQYLAMSFRILALIAAGFAIQSALRVRSEETAMHAEPVLATPVSRNRFAG
                     AHLAIAFGGTLVVLLLVGLSFGIADAAVTGDADAIWTSIVGACVYAPAVWTMIGLVVA
                     AIGLAPRLSAWPWGVLGICFVIGMFGQLLDLPTWVEDLSPFQHVPQYPANELDIVPLV
                     VLVAIAAGLTAVGLAGLRRRDIG"
     gene            complement(1782..2396)
                     /locus_tag="VFX21_11345"
     CDS             complement(1782..2396)
                     /locus_tag="VFX21_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096604.1"
                     /translation="MTLDAYRRDDRTAVDDAAAREAWTRAARPVLVQVARRYGSLMKP
                     GDLAEEVQGVTSVRTRVPAEEWIDDVLDAVDVDCVARDEPLLSSFCVGADGRIGDRYR
                     RLVSGLDPTATDVEMHAATQRLEAHRYHGAIMPVDGGKPALPPQLAKLRASAPKSTAT
                     RTRATRAKAPAPRRPKKPDLAERPVCPTCFLQLPATGRCDNCDE"
     gene            complement(2420..2842)
                     /locus_tag="VFX21_11350"
     CDS             complement(2420..2842)
                     /locus_tag="VFX21_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005243574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX5096605.1"
                     /translation="MAAKSRKARVEDVHELARAMPHVTVDRGPKSNDVYQVGGKSFVF
                     FRTPRPDAFDPETGERYDDVIVFWVPSEADKLALVQDESSPFFTTPHFDGHPSVLVRA
                     SRLGEITRDELQEIVEDAWLSQASARRAATWLAEHRTD"
     gene            2878..3897
                     /locus_tag="VFX21_11355"
     CDS             2878..3897
                     /locus_tag="VFX21_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HEX5096606.1"
                     /translation="MVTHEHELTDPVDLCTPDGAHLNPAARGWSRAPLHRANLHGEFG
                     RNKRWDYWAVLAGDLVVSAVYSDIDVFGLADVWWADLEHGATGGRAILVPSDAVELPE
                     RPGTAPLRVDRDGLDLSITDDDRGTRLSARWHETDGRPGRLDVLVALPAGHESLNVVI
                     PWSDNEFNYTSKHQARPATGTLTVGDRAWEIGWGAWGVLDVGRGRWPHEITWNWGGGA
                     GRAGDHVVGLQFGAKWTAGTGFTENGVIVDGRLSKIGNELDWQYDWEAPMEPWRVSDP
                     GGQLDVILTPRFDKHSKVGDDGKYGSETHQVFGTWSGVVRTDDGVRIEIEGLQGFAEE
                     ARQRW"
     gene            3925..>4694
                     /locus_tag="VFX21_11360"
     CDS             3925..>4694
                     /locus_tag="VFX21_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096607.1"
                     /translation="MVRSTPGSELIEQARAALRAGDAASARRLLDEAASERPDDPSVA
                     ECLARTAYLERDFTGASEHWQRAYAGYRACADQAGAVRVARSLAPLYGMVFGDGAVMS
                     GWLERAQTLLGEESDSPEAGWVALNIGMFESDRARKNEHFRRALTIARRLGDVDLEIV
                     SLAYLGASLVHANRTDEGMRLLDESLAAVAGAEVESFQVLEEVFCQLFSACEHAHDVA
                     RADQWIRIGEQIAARRQLPSVAAFCHTHYGGLLTVAGR"
BASE COUNT          665 a         1634 c         1655 g          740 t
ORIGIN      
        1 cttcgacgtc gacaccgtgc acctcgagga agcagtccgc cgcctcgccg atctgcacgt
       61 gcggagcctc gtgagccatc cgccgacact cgaggaactg ttcctccgct actacggcga
      121 cgagctgacc cacgagtccg agctcgcgca tctgttcgac gacgagtcat gaccgcgacc
      181 gcgtctccct tcaccggtct cgcgccgctg agccggttca ccatccgccg tgaccgcgtg
      241 cgcatcgtcg tctggatcgc cgcgatcgtg ctgctcgtgg tcgtcaccgt cgccagcgtc
      301 aaaggcctgt atccgaacca ggaggagctc gacaaggcgg cgcgcgcctc cgaggacaac
      361 gtcgccgcga tcatcttcaa cgggccgccg caagcgctcg acaccgtcgg cggccaggtc
      421 gcgttccaga ccggaacgtg gggcttgatc ctgatcggga tgatgagcat cttcatgctc
      481 gggcggctca cgcgcggcga ggaagaggcg ggtcgcaacg aactgttgcg ttcgctcccc
      541 atcggtgcgc actcgctgcc cggcgcggcg ctgctcacgg tcggtgcgat gaacgtcgtc
      601 accggggcgc tggtcacgat cgtgctgctc gcgctgtcgc tcccggcgac cggctccgtc
      661 gtgttcggcc tgtcgttcac gatgttcggg ctcttgctcg ccgcggtcac gctcgtggcc
      721 gcacagatca ccgagaacac gcgcgtcgtg tacggcctcg gcggcgtcgt gctcggtgcg
      781 tcgttcgtgc tgcgcgcgat cggcgacatc ggtgacggca ccgtgtcgtg gctgtcgccg
      841 atcggctggg cgcagaagac ccgaccctat gcgggtgaga actggtggcc gttcttgctc
      901 atcgccgtcg cgacgggcgc gctcgcatgg gtcgcgggcg tgctgtcgcg gcatcgcgat
      961 ctcggttccg ggctcatcgc accgcgaacc ggacgcgcgc gagcagcccc gtcgctgggc
     1021 accccactcg gcctcgcgac cagagtgcag cgcgggagcc tgctgggctg gagcgccggc
     1081 ctgctcttcc tcgcggtcgc ctacggctcg atcaccgact cgatcgacca gttcgtcgag
     1141 gacaacgatg cgctcaacga catcatcgcc gcgcaaggca acggcacgct ggtcgagcag
     1201 tacctcgcga tgtcgttccg catcctggcc ctcatcgcgg ccggcttcgc gatccaatcc
     1261 gcgctgcgcg tccgcagtga agagacggcg atgcacgccg agccggtgct ggcgacgccc
     1321 gtttcgcgca accgcttcgc gggcgcccat ctcgcgatcg cgttcggcgg cacgctcgtc
     1381 gtcctgctgc tcgtcgggct gtcgttcggg atcgcggatg ccgcggtgac cggcgacgcg
     1441 gacgcaatat ggacgtcgat cgtcggcgcg tgcgtctacg cccctgcggt gtggaccatg
     1501 atcggactcg tcgtcgcggc gatcggcctc gcgccgcggc tcagcgcgtg gccgtggggc
     1561 gtgctcggca tctgcttcgt gatcgggatg ttcggccagt tgctcgacct gccgacgtgg
     1621 gtcgaagatc tgtccccgtt ccagcacgtg ccgcaatacc cggcgaacga gctcgacatc
     1681 gtgcccctcg tcgtcctcgt cgcgatcgcg gccggtctca ccgcggtcgg actcgccggt
     1741 ctgcgccgcc gcgacatcgg ctgacaccgc ggcgccgcct gttactcgtc gcagttgtcg
     1801 cagcggcccg tcgccggcag ctggaggaag cacgtagggc acaccggccg ttcggcgagg
     1861 tcgggcttct tgggtctgcg cggcgcgggc gctttggcgc gcgtcgcacg cgtgcgcgtg
     1921 gccgtcgact tcggtgcgga cgcgcgcagc ttcgcgagct gcggtggcaa cgccggcttc
     1981 ccgccgtcga ccggcatgat cgcgccgtgg tagcgatgcg cttcgagccg ctgcgtcgcg
     2041 gcgtgcatct cgacgtcggt cgcggtcggg tcgagacccg agacgagccg tcggtaacgg
     2101 tcaccgatcc gcccgtcggc gccgacgcag aacgacgaca gcagcggctc gtcgcgcgcg
     2161 acgcagtcga catcgaccgc gtcgagcacg tcgtcgatcc actcctcggc cgggacgcgc
     2221 gtacggacac tcgtgacgcc ttgcacctcc tcggcgaggt cgccgggctt catcaacgag
     2281 ccgtaccgcc gcgcgacctg caccagtacc ggccgggcgg cgcgcgtcca ggcttcgcgc
     2341 gcggccgcgt cgtcgaccgc cgttcggtcg tcccggcggt aggcgtcgag cgtcatcagg
     2401 gtcgcaccgt agcgattcgt cagtcggtgc gatgctcggc gagccacgtg gcggcccggc
     2461 gtgcggacgc ctgcgagagc caggcgtcct cgacgatctc ctgcagctcg tcgcgggtga
     2521 tctcgccgag gcggctcgcc cggaccaaga ccgacgggtg cccgtcgaag tgcggcgtgg
     2581 tgaagaaggg cgacgattcg tcctggacga gcgcgagctt gtcggcctcg gacggcaccc
     2641 agaacacgat cacgtcgtcg tagcgctcgc cggtctcggg atcgaacgca tcgggccggg
     2701 gcgtgcggaa gaacacgaac gacttgccgc cgacttggta gacgtcgttg ctcttcggcc
     2761 cgcgatcgac ggtcacgtgc ggcatcgccc gtgcgagctc gtgcacgtcc tcgacccgcg
     2821 ccttgcgcga cttggccgcc acgcaggcca gcctacggag atagcgtcgg gcgcgtcatg
     2881 gtcacccacg agcacgagct gaccgatccc gtcgatctct gcactcccga cggcgcgcac
     2941 ctcaacccgg ccgcgcgcgg ctggtcgcgc gcccccctgc accgcgcgaa cctgcacggc
     3001 gagttcggcc ggaacaagcg gtgggactac tgggcggtgc tcgcgggcga cctcgtcgtg
     3061 tcggccgtct actccgacat cgacgtgttc gggttggccg acgtgtggtg ggccgacctc
     3121 gagcacggtg cgacgggcgg cagggcgatc ctcgtgccga gcgacgccgt cgagctcccc
     3181 gaacgaccgg gaaccgcgcc gctgcgcgtc gaccgcgacg gcctcgacct gtcgatcacc
     3241 gacgacgacc gcggcacgcg cttgtcggcc cggtggcacg agaccgacgg ccggcccggt
     3301 cgtctcgacg tgctcgtcgc gctgcccgcc ggccacgagt cgctgaacgt cgtcatcccg
     3361 tggagcgaca acgagttcaa ctacacgtcg aagcaccaag cgcggcccgc gaccggcacg
     3421 ctcaccgtcg gcgaccgcgc atgggagatc ggatggggcg cgtggggtgt gctcgacgtc
     3481 gggcgcggac gctggccgca cgagatcaca tggaactggg gtggcggcgc gggccgtgcc
     3541 ggtgatcacg tcgtcggtct gcagttcggc gcgaagtgga ccgcgggcac cgggttcacc
     3601 gagaacggcg tgatcgtcga cggccggctg tcgaagatcg gcaacgagct cgactggcaa
     3661 tacgactggg aggcgccgat ggagccgtgg cgcgtcagtg atcccggcgg tcagctcgac
     3721 gtcatcctca cgccgcgctt cgacaagcac tcgaaggtcg gcgacgacgg gaagtacggc
     3781 agcgagaccc accaagtgtt cggcacgtgg tcgggtgtgg ttcgcaccga cgacggcgtg
     3841 cgcatcgaga tcgagggact gcaagggttc gccgaggagg cgcgccagcg ctggtgagca
     3901 gcggacgaag agaaggggcg accgttggtt cggtccacgc ccgggagcga actgatcgag
     3961 caagcgcgcg ccgcactgcg agcgggtgac gcggcgagcg ctcgccgcct gctcgacgaa
     4021 gcggcttccg agagacccga cgacccgagc gtcgcggaat gcttggctcg cacggcgtac
     4081 ctcgagcgcg acttcaccgg cgcgagtgag cactggcagc gcgcgtacgc cggctatcgc
     4141 gcgtgcgccg accaggcggg cgcagtgcgc gtcgcccgtt cgctcgcacc gctgtacggc
     4201 atggtgttcg gcgacggcgc ggtcatgagc gggtggctcg agcgcgcaca gacgctgctg
     4261 ggcgaggagt cggactcacc cgaagcgggc tgggtcgcgc tcaacatcgg catgttcgag
     4321 agtgatcgcg cccgcaagaa cgagcatttc cgccgtgcgc tgacgatcgc acgccggttg
     4381 ggcgacgtcg acctcgagat cgtcagcctc gcctacctcg gcgccagcct cgtgcacgca
     4441 aaccgaaccg acgaggggat gcggctgctc gacgaatcgc tggcggcggt ggccggtgcc
     4501 gaagtcgaga gctttcaagt gctggaggaa gtcttctgtc agctgttctc cgcgtgcgaa
     4561 cacgcccacg acgtcgcgcg cgcggaccag tggatccgga tcggcgaaca gatcgccgcc
     4621 cgccgccagc ttccctccgt ggcggcgttc tgtcacacgc actacggcgg tctgttgacc
     4681 gtcgccggtc gatg
//
LOCUS       DASUGA010000576         5594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1832133, whole genome shotgun sequence.
ACCESSION   DASUGA010000576 DASUGA010000000
VERSION     DASUGA010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832133"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VFX21_11365"
     CDS             <1..439
                     /locus_tag="VFX21_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096608.1"
                     /translation="PLESVFLWTPAALACTDLILNGTLEKFPDLHIGVVELSAIWVPM
                     YLMMLDGGYDFTTKLNGRPLAPLALRPSEYFKRQVRVSSFSYELPGRLIRQTGDLYMC
                     CSDYPHSEGTATPLADYTGSALPVRPEDAPGLFHDNVSLLLGA"
     gene            complement(436..894)
                     /locus_tag="VFX21_11370"
     CDS             complement(436..894)
                     /locus_tag="VFX21_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5096609.1"
                     /translation="MGYTPQEVADRAEIHDVIVRYGWAIDTKDWALLDTVFTEDAYLD
                     YTSNPGGKEGPYPKVRAWLAKVMAAFPVTQHLMANIDVQLDGDRATVRTMVTNPQGAA
                     TREGPLHFFYVGARYDDEFVRTPSGWRIAERVETTLWFEGSLPPELLFPD"
     gene            complement(900..1379)
                     /locus_tag="VFX21_11375"
     CDS             complement(900..1379)
                     /locus_tag="VFX21_11375"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5096610.1"
                     /translation="MALTELKGRPLGTQKVAIERGPVRVFAQALMDDDAAYTGDAAPV
                     PPTFPFVMSYWGSMGQGGAAGLPIENLRGKGRAILHGEQEFVFHDGRWPHVGDVLVGE
                     GVISDVYEKERSDGGKLEFYVTDTTWSNEATGEPVVTAKFTLTIVCKPGADEPKPLS"
     gene            complement(1405..1788)
                     /locus_tag="VFX21_11380"
     CDS             complement(1405..1788)
                     /locus_tag="VFX21_11380"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX5096611.1"
                     /translation="MKRQQSSGVMIQGRSSGQRRAHVRVPVCTPMRLTAEENGRVTQL
                     TGHVLDVSITGCAARVYAVLEPDMVVRLAFEMDGEPMQIRGRIMWSKTKGGGRLIGIR
                     FEGVRTQQAVALQNYIAQQQGRRLL"
     gene            complement(1906..3960)
                     /locus_tag="VFX21_11385"
     CDS             complement(1906..3960)
                     /locus_tag="VFX21_11385"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX5096612.1"
                     /translation="MSGRTAAGGRAEAPGDTWAWRALLATTVAVPLAFTPFTADVYMG
                     VKVWLTVAGVAFVGAGVVVGQRRLPPWPRVAWAVVGLGAVSLLAAFVGRTPATSLAGA
                     YQRYGGVSSLVVFVAFAALLVVCTWDRPERVATLQLFLAGTAVAAASYALLQRAGIDA
                     FDVREASGANARYPGSTLGNSNFAGGFFAIALPCVVACAVRAADRRYRLVWSAAVAIV
                     AGGLWASSSRGGMIAAAVGLAVAAIAGRHAITARLRPLTFVAGAVAVVLVGVGIGVIV
                     RAGDDVPSSDLDVLRSQSAQARHFEWEAALDGIVDRPLLGNGPDSFAVVYPLERSAED
                     GASAGNIRADKPHNVFLERASDTGVLGIAAYLAVIGIVLVGAWRRARHVTGRDLVVLV
                     AFVGSLGAYLTQALFSIDVPPLAWTGWMLVGALVALSDPEIVAARAAKPPTVKAARVS
                     AARAAGPWAVAGIITLLAALPIAGDARLHFAENDDRDGDLEAAFDGYDAAAGLRPFEP
                     EYRRASGFAAERLAAATDDRATKKHWFTIAMNRYQDALDRRPGNVFFLSDLGRVQALY
                     AQQIDPKLYADADATLTDAVAADPHNWELRETHGVVLNAWANASGDTNVRRRAANEYE
                     EVVALQPKHYSAWITLGRLRLALGDAAGAKEAADTAGILVPFTQDAAELWEAADALAA
                     QG"
     gene            4084..>5594
                     /locus_tag="VFX21_11390"
     CDS             4084..>5594
                     /locus_tag="VFX21_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX5096613.1"
                     /translation="MKRAWVYVLAGIVGAASIPFGAGPAGADVGPGPDNDNRDHAWSD
                     WAPQAVTIDLSDANRDGAASACGAPAGGDIWWNLNQYTDGEVILKATAVGGDVDPIIE
                     ILDSHDGVITCNDDMSAQTTNAEVRFVGDANDARWIRIVAAAGTHGSVQVTWQRPATS
                     FIEGVVRADGTGEPLSDICVSAFSSGDWRFSYSGQAVTSATGSFRIGPVNASGYNLSF
                     SDCSNRPDRYVAEYWLDADGFTQPTTVLAFDNQTTTLSPDIRLGLGGTIAGQVTILGA
                     GGKGARDVCVNVVGETGFFGNFTLTNQTGEYRVGGLPTGTYKVLFSDCSDARLLASEW
                     YHDVYGDFSARDRATAVSVTGGATTTVDEDMRTGGVISGTVSAALPAPPGYVYVDVYD
                     ADGEFVTGTSVQSGQYRITGLPGGDYKVKFTGYDAATFGEVASEWWNDQPASDAATPV
                     TVEWGIARFGIDAVLGGGRIVGTVTDTDTGDPLPDKCVQAQNGVVSRSTTTSST"
BASE COUNT          882 a         1962 c         1917 g          833 t
ORIGIN      
        1 gccgctcgag tcggtgttcc tctggacgcc ggccgcgctc gcgtgcaccg acctcattct
       61 caacggcacg ctcgagaaat tccccgactt gcacatcggc gtcgtcgagc tgtcggcgat
      121 ctgggtgccg atgtacttga tgatgctcga cggtggctac gacttcacga cgaagctcaa
      181 cggccgcccg ctcgcgccgt tggcgctgcg ccccagcgag tacttcaagc gtcaggtgcg
      241 cgtgtcgtcg ttctcctacg agctcccggg ccgcctcatc cgccaaaccg gcgacctcta
      301 catgtgctgc tccgactacc cgcattccga agggaccgcg acgccgctcg ccgactacac
      361 cggcagcgcc ttgccggtac gccccgaaga cgcgcccggc ctgttccacg acaacgtctc
      421 gctgttgttg ggcgcttagt cggggaacaa cagctccggc gggagcgagc cttcgaacca
      481 gagcgtcgtc tcgacgcgct cggcgatgcg ccagccggac ggcgttcgga cgaactcgtc
      541 gtcgtaacgc gcaccgacgt agaagaagtg caacggtcct tcgcgcgtcg ccgcgccctg
      601 ggggttggtc accatcgtgc gcacggtcgc gcggtcgccg tcgagctgca cgtcgatgtt
      661 cgccatcaag tgctgcgtga ccgggaacgc ggccatgacc ttcgccagcc acgcgcgcac
      721 cttcgggtac ggcccttctt tgccacccgg gttcgacgtg tagtcgaggt acgcgtcctc
      781 ggtgaagacc gtgtcgagca acgcccagtc cttggtgtcg atcgcccagc cgtagcggac
      841 gatcacatcg tggatctcgg cccggtcggc gacctcctgc ggcgtgtacc ccacgggcgc
      901 tacgagagcg gcttgggctc gtcggcgccg ggcttgcaga cgatcgtcag cgtgaacttc
      961 gcggtgacga cgggttcgcc cgtggcttcg ttcgaccacg tcgtgtcggt cacgtagaac
     1021 tcgagcttgc cgccgtcgga gcgttctttc tcgtacacgt cggagatgac gccttcaccg
     1081 acgagcacgt cgccgacgtg cggccaccgg ccgtcgtgga acacgaactc ctgctcacca
     1141 tgcaggatcg cgcggccctt gccgcgcagg ttctcgatcg gcaggccggc cgcgccaccc
     1201 tggcccatcg aaccccagta cgacatgacg aacgggaacg tcggcggcac gggcgcggcg
     1261 tcgcccgtgt acgccgcgtc gtcgtccatc aacgcctgcg cgaacacgcg cactgggccg
     1321 cgctcgatgg cgaccttctg ggttccgagc gggcgtccct tgagctcagt caaagccatg
     1381 ggccgggaga ctagcgggag tggttcagag gagccgacgg ccctgttgtt gggcgatgta
     1441 gttctgtagc gcgacagctt gttgcgtccg gacaccttcg aaacggatgc cgatcaagcg
     1501 cccgccaccc ttggtcttcg accacatgat ccggccccgg atctgcatcg gctcgccgtc
     1561 catctcgaac gccaaacgga cgaccatgtc gggctccagc accgcgtaga cgcgcgccgc
     1621 acagccggtg atggagacgt cgagcacgtg gcccgtgagc tgtgtgacgc ggccgttctc
     1681 ctcggcggtg agtcgcatcg gtgtgcacac gggcacgcgt acgtgcgctc gccgctgccc
     1741 ggaggagcgg ccctggatca tgacgcccga ggactgttgc cgcttcatag gagaattctc
     1801 ggaatcggaa cccgccgcgg agaagcccga actttgtgaa ccttcgcacg ttcggcgggc
     1861 cgcagggtgg cgcgggcgca tttcggtggc gggcggtgac ggaggtcacc cctgtgcggc
     1921 gagcgcgtcg gctgcttccc acagctcggc cgcgtcctgc gtgaacggca cgaggatccc
     1981 cgccgtgtcc gcggcttcct tggcgccggc ggcgtcgccg agcgcgagcc ggagccggcc
     2041 gagggtgatc cacgcgctgt agtgcttcgg ctggagggcg acgacctctt cgtactcgtt
     2101 cgcggcgcgt cggcggacgt tggtgtctcc actcgcgttg gcccacgcgt tcagcacgac
     2161 gccgtgggtc tcgcgcagct cccagttgtg cgggtcggcg gcgacggcgt cggtcagcgt
     2221 ggcatcggca tcggcataca gcttgggatc gatctgctgc gcgtacagcg cctgcacgcg
     2281 cccgaggtcg ctcaagaaga agacgttgcc gggccggcgg tccaacgcgt cttggtagcg
     2341 gttcatcgcg atggtgaacc agtgcttctt cgtcgcgcgg tcatcggttg cggccgcgag
     2401 ccgttcggcg gcgaaccccg acgcgcgccg gtactccggt tcgaacgggc gcagaccggc
     2461 ggccgcgtcg tagccgtcga acgcggcctc gagatcgccg tcgcggtcgt cgttctcggc
     2521 gaagtgcagt cgcgcgtcgc cggcgatcgg gagcgccgcg agcaacgtga tgatcccggc
     2581 gacggcccac ggcccggcgg cccgcgcggc cgagacgcgc gcggccttga ccgtgggcgg
     2641 cttcgcggca cgcgccgcga cgatctccgg atcggagagt gcgacgagcg cgccgacgag
     2701 catccatccc gtccacgcca acggcggcac gtcgatcgag aacaatgctt gtgtcagata
     2761 ggcgcccaac gaaccgacga acgcgacgag cacgaccagg tcgcggccgg tgacatgccg
     2821 cgcgcggcgc cacgcaccga ccagcacgat gccgatcacc gcgaggtacg cggcgatgcc
     2881 gagcacgccg gtgtcgctcg cccgttcgag gaacacgttg tgcggcttgt cggcgcggat
     2941 gttgccggct gatgcgccgt cctccgcgct gcgctcgagc gggtacacga cggcgaacga
     3001 gtcgggaccg ttgccgagca gcggccggtc gacgatcccg tcgagtgcag cctcccattc
     3061 gaagtgccgc gcctgcgcgc tctggctgcg caggacatcg agatcgctgc tcggtacgtc
     3121 gtcgccagcg cgcacgatga cgccgatgcc cacaccgacg aggacgaccg cgaccgcgcc
     3181 cgcgacgaag gtcagcggtc gtagccgcgc tgtgatcgcg tgccggcccg cgatcgcggc
     3241 gaccgcgagc ccgacggccg cggcgatcat gccgccgcgg ctcgacgacg cccagagccc
     3301 acccgcgacg atcgcgaccg cggccgacca gacgagccgg taccggcggt ccgcggcgcg
     3361 cactgcgcac gcgacgacgc acggcaacgc gatcgcgaag aacccgcccg cgaagttcga
     3421 gttgccgagg gtcgagcccg ggtagcgcgc gttggcaccc gaggcttcgc ggacgtcgaa
     3481 cgcatcgatg ccggctcgct gcaagagcgc gtacgacgcg gccgcgacgg cagtaccggc
     3541 caggaagagc tgcagtgtgg cgacccgctc gggccggtcc caggtgcaca cgaccagcag
     3601 cgccgcgaac gcgacgaaga ccacgagcga ggagacaccg ccgtagcgct ggtaggcccc
     3661 ggccagcgac gttgccggcg tccggcccac gaacgccgcg aggaggctca ccgcgccgag
     3721 cccgacgacc gcccacgcga cccgcggcca cggcgggagc cggcgctgcc cgaccacgac
     3781 gcccgcgccg acgaaggcga cgccggccac cgtcagccag accttgacgc ccatgtagac
     3841 gtcggccgtg aaaggcgtga aggcgagcgg caccgcgacg gtggtggcca ggagggcgcg
     3901 ccaggcccag gtgtctcccg gcgcttcggc gcgcccgccc gctgccgtcc gcccggacat
     3961 cgctcaatcc tcgcgcgaga gatccgacat tcttcgcaac cttccggcct cgggcaacgt
     4021 catgtcacca gagcgcgcat ccatacatcg gcgcgtcacg ggcggcaggg agccagaatc
     4081 accatgaagc gggcgtgggt ctacgtgctg gcgggcatcg tcggcgcggc atcgattccc
     4141 ttcggcgccg gcccggccgg cgcggacgtc gggccggggc ccgacaacga caaccgagac
     4201 cacgcctgga gcgactgggc gccgcaggcc gtcacgatcg acctctccga cgcgaaccgc
     4261 gacggcgccg cgagcgcatg cggcgcaccc gccggcggtg acatctggtg gaacctcaac
     4321 cagtacaccg acggcgaggt catcctcaag gccacggcgg tcggcggcga cgtcgacccg
     4381 atcatcgaga tcctcgactc gcacgacggc gtcatcacct gcaacgacga catgtccgcg
     4441 cagacgacaa acgccgaggt gcggttcgtg ggcgacgcga acgacgcgcg ctggatccgc
     4501 atcgtcgccg ccgctggtac gcacggctcc gtgcaagtga cgtggcagcg cccggcaacg
     4561 agcttcatcg aaggtgttgt gcgcgccgac ggtacgggcg agccgctctc cgacatctgc
     4621 gtcagtgcgt tctcgtccgg cgactggcgc ttctcctaca gcggacaggc ggtgacgtcg
     4681 gccacggggt cgttccgcat cggtccggtc aacgcgtcgg gctacaacct ctcgttcagt
     4741 gattgcagca accgacccga tcgttacgtc gctgagtact ggctcgacgc cgacggattc
     4801 acgcaaccga ccaccgtgct tgcgttcgac aaccagacga cgacgctgag tcccgacatt
     4861 cgcctcggtc tcggtggcac gatcgccggg caggtcacca tcctgggcgc gggcgggaag
     4921 ggcgcgcgcg atgtatgcgt caacgtcgtc ggcgagaccg gattcttcgg gaacttcaca
     4981 ctgaccaacc agaccggtga ataccgcgtc ggcgggcttc caactggcac gtacaaggtc
     5041 ttgttctccg actgcagtga cgcccgtctt ctcgcgagcg agtggtacca cgacgtctac
     5101 ggtgacttca gcgcacgcga ccgggcgacc gcggtgtccg tcacgggcgg cgcgacgacc
     5161 accgtggacg aggacatgcg caccgggggc gtcatctccg ggacggtgtc cgctgcgctg
     5221 ccggcgccgc cgggatacgt gtacgtcgac gtgtacgacg ccgacggcga attcgtgacc
     5281 ggcacatcgg tgcagagcgg tcagtaccgc atcaccggat tgccgggtgg cgactacaag
     5341 gtcaagttca ccggttacga cgcggcgacg ttcggcgaag tggcctccga gtggtggaac
     5401 gaccaacctg cgagcgatgc ggcgacgccg gtcaccgtcg agtggggcat cgcacggttc
     5461 ggcatcgatg ccgtgctcgg tggcggccgc atcgtgggca cggtcaccga caccgacacc
     5521 ggcgacccgc ttcccgacaa gtgcgtgcag gcgcagaacg gcgttgtgag ccgctcgacc
     5581 acgacgtcgt cgac
//
LOCUS       DASUGA010000577         2498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_285250, whole genome shotgun sequence.
ACCESSION   DASUGA010000577 DASUGA010000000
VERSION     DASUGA010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285250"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VFX21_11395"
     CDS             complement(<1..761)
                     /locus_tag="VFX21_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX5096614.1"
                     /translation="MLPDGLIAIVKKACPTCEMVAPVLEQLGAADRLTVYSQDDPAFP
                     AGLAAIDDTALDVSFALDIDTVPTLMRVSGGEVTERIVGWERMQWEKLTGVAGLGPGL
                     PQYKPGCGSRTQEPEIQDARAVARMLPKLQARRIELGDAEDEMEAMYARGWSDGLPLV
                     APTPERVARMLAGTHRDPSEVVVLAPPDLVECTVEKIAINAVMAGCAPEYLPVVLTAV
                     EAACTDEFNMHGLLATTWFAGPVVIVNGPIARRIG"
     gene            complement(767..1885)
                     /gene="serC"
                     /locus_tag="VFX21_11400"
     CDS             complement(767..1885)
                     /gene="serC"
                     /locus_tag="VFX21_11400"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEX5096615.1"
                     /translation="MTADIALPADLLPSDGRFGSGPSKVRTDSVAALAKAAPHYMGTS
                     HRRDTVRNVVGRVRDGLAELFALPDGYEVLIGNGGSTLFWDAASFGLIEEKSSHLVLG
                     EFSSKFASVAKKAPHLGAPEVLETEPGATPKLLDAPTGDAYAYPHNETSTGVSLPLHR
                     PSGDALVLVDATSAAGGTRVDPSLFDVYYFAPQKCFAGDGGTFLALASPAAIERIERI
                     GATGRWVPPSLDLGLVVENSRQNQTYNTLALATVFLLAEQIEWMLGNGGLEWAVNRCD
                     TSARTIYDWADAHPHAQPFVTDPAARSHVTATIDFDDSVDAAALAKTLRANGIVDTEP
                     YRKLGRNQLRIALFPAIEPDDVATLTRAIDYIVERLSD"
     gene            complement(1950..>2498)
                     /locus_tag="VFX21_11405"
     CDS             complement(1950..>2498)
                     /locus_tag="VFX21_11405"
                     /inference="COORDINATES: protein motif:HMM:NF018657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HEX5096616.1"
                     /translation="AAVLGGLRGYHARHGAAPNELRMTLPINLRQRGDVTAGNRFAPA
                     RFAVPADLEDPRDRVEAIGALVRSWRSEPALQMTGTLAGVLNRLPTAATTALFGSMLK
                     CCDFVTTNVPGAPVPVYAAGARVEGLYAFAPPSGAAVNVSLISHIDTCCIGVVLDTTA
                     VPDGDVLVRCLREGFDDVLALG"
BASE COUNT          378 a          838 c          904 g          378 t
ORIGIN      
        1 atgccgatgc ggcgcgcgat cggaccgttg acgatcacga ccggacccgc gaaccacgtc
       61 gttgccagga ggccgtgcat gttgaactcg tcggtgcacg cagcctcgac cgcggtgagc
      121 acgacgggca ggtactcggg cgcgcagccg gccatgaccg cgttgatcgc gatcttctcg
      181 acggtgcact cgacgaggtc gggcggcgcc agcacgacga cctcggaggg atcgcggtgc
      241 gtgcccgcga gcatgcgcgc cacccgctcg ggtgtcggtg cgacgagcgg caggccgtcg
      301 ctccacccgc gtgcgtacat cgcttccatc tcgtcttcgg cgtcacccag ctcgatccgc
      361 cgcgcctgca gcttcggcag catgcgcgcg acggcgcgcg catcctggat ctccggttct
      421 tgcgtacgcg agccgcagcc cggcttgtac tgcgggagcc cggggccgag gccggcgacg
      481 cccgtgagct tctcccactg catgcgttcc cacccgacga tgcgctcggt cacctcgccg
      541 ccgctgacgc gcatgagtgt gggtaccgtg tcgatgtcga gcgcgaacga cacgtcgagc
      601 gcggtgtcgt cgatcgcggc gagcccggcc gggaacgcgg gatcgtcctg cgagtacacc
      661 gtcagccggt cggcagcgcc gagttgttcg aggaccggcg cgaccatctc acaggtcggg
      721 caggccttct tgacgatcgc gatcaagccg tcggggagca cgcccctcag tctgacaaac
      781 gctcgacgat gtagtcgatg gcccgggtga gcgtggccac gtcgtcgggt tcgatcgcgg
      841 ggaacaaggc gatgcgcagc tgattgcggc cgagcttgcg gtacggctcg gtgtcgacga
      901 tgccgttggc ccgcagtgtc ttggccagtg ccgcggcgtc gaccgagtcg tcgaagtcga
      961 tggtcgcggt gacgtggctg cgcgcggccg gatcggtgac gaacggctgc gcgtgaggat
     1021 gcgcgtcggc ccagtcgtag atcgtgcgag ccgacgtgtc gcagcggttc acggcccact
     1081 cgaggccgcc gttgccgagc atccactcga tctgctccgc gaggaggaag accgtggcga
     1141 gcgcgagcgt gttgtacgtc tggttctgac gcgagttctc gacgaccaag cccagatcga
     1201 gcgacggcgg gacccaacga ccggttgcgc cgatgcgttc tatgcgctcg atcgcggcgg
     1261 gcgacgcgag cgcaaggaac gtgccgccgt caccggcaaa gcacttctgg ggggcgaagt
     1321 agtagacgtc gaacaacgag ggatcgacgc gggtgccgcc cgcggcggac gtcgcgtcga
     1381 cgaggacgag cgcgtcgccg ctcgggcggt gcaacggcag gctcacaccc gtcgacgtct
     1441 cgttgtgcgg gtacgcgtac gcatcaccgg tcggcgcgtc gagcagcttc ggtgtggcgc
     1501 cgggctccgt ctcgagcact tcgggtgcgc cgaggtgggg cgccttcttc gcgaccgacg
     1561 cgaacttcga cgagaactcg ccgagcacga ggtgtgagct cttttcctcg atcagtccga
     1621 acgacgccgc gtcccagaac aacgtggatc cgccgttgcc gatcagcact tcgtagccgt
     1681 cgggcagcgc gaacagctcg gcgagaccgt cgcgcacgcg cccgacgacg ttgcgcacgg
     1741 tgtcgcggcg gtggctcgtg cccatgtagt gcggcgcggc cttggccagc gcggcgaccg
     1801 agtcggtgcg gaccttcgac ggccccgaac cgaaccgtcc gtcgctcggg agcaggtcgg
     1861 cggggagggc gatgtcggcg gtcaccgcgt cacgataacg gctcgacccg cgccgcctcg
     1921 ggtcgatttc cggtgtgcgg gttgcgagct cagccgagcg cgaggacgtc gtcgaagccc
     1981 tcgcgcaggc agcggacgag cacgtcgccg tcggggaccg cggtcgtgtc gagcacgacg
     2041 ccgatgcagc acgtgtcgat gtgcgagatc agcgagacgt tcaccgccgc acccgacggc
     2101 ggcgcgaacg cgtacagccc ctcgacgcgc gcgccggcgg cgtagaccgg caccggcgcg
     2161 ccgggcacgt tcgtcgtgac gaagtcgcaa cacttgagca tgctgccgaa gagcgcggtg
     2221 gtcgccgccg tcggaagccg gttgagcacg ccggcgagcg tgccagtcat ttgcagcgca
     2281 ggctctgacc gccagctccg gacgagcgcg ccgattgcct cgacgcgatc acgtgggtcc
     2341 tcgagatcag cgggtaccgc gaagcgcgcc ggtgcgaacc gattgccggc ggtgacatca
     2401 ccacgttgcc gcaggttgat cggcaacgtc atccgtaact cgttcggcgc cgcgccgtgg
     2461 cgggcgtgat agccacgaag tccgccgagc accgcggc
//
LOCUS       DASUGA010000578         2026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1097466, whole genome shotgun sequence.
ACCESSION   DASUGA010000578 DASUGA010000000
VERSION     DASUGA010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097466"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1074
                     /locus_tag="VFX21_11410"
     CDS             142..1074
                     /locus_tag="VFX21_11410"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX5096617.1"
                     /translation="MATPEVPAEMIRRPPARAVRWVEAAVGRGARVVRTRRLRNAWAA
                     AMHAVDVEHDGRRHALVLRRWVRRDFPPDVGVVDNEAAVLSLLATHDLSVPRLVAADP
                     AGTECDVPAVLMTRLTGRDELSPADLDGWLDGLAAAHAAIRTVAVPPGVLGYYRPWGW
                     DTVTAPPPWSSRPDAWARAIEIVHSGVPATELVLCHRDFHPGNVLWSRGRVSGIVDWS
                     HACRGAPAADIAHCRMNLNTLFDLDAADAFTARCAPVEHLPWFDLADVVSLGESPPEV
                     WRFHDAGRTDLTVRSMIAATDAFVVQGVERYDGV"
     gene            1153..>2026
                     /locus_tag="VFX21_11415"
     CDS             1153..>2026
                     /locus_tag="VFX21_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096618.1"
                     /translation="MGAFALVIGLTAVLSWRLPYAGDEASHLVMVRHHAHHLDLVSWD
                     EMQYGGERGHSYFLFSPLPYAVYVPFALLDEHVGDLPGAVRPDRELTRLGGGIVLAAA
                     QLALTLALVRRMIRSSDQTTVTAIALAANLLPQLRYIHAYPNTDGVTILAGTACFWAA
                     LRVLQSERVGLRDAALVGVLIGVTMHVRFQAFLAAAVLLAAFAVRALQQSVSLRPRLR
                     LLGVAVAIPVLLAGSFHLAIYDELDNGHLLPTVDNEQLNDSTYSGVLVAEPAAKSLVS
                     RRVNQIPEVWMGVWA"
BASE COUNT          269 a          736 c          687 g          334 t
ORIGIN      
        1 gcacgtagtg acgggcgggc acggggaagg gccgctgcgc gcgaccgggg agcgcgtcgg
       61 cggcggcgat catcgtctgc ttgttgcgtc cgaacatgac acctctctcg aaacgaagag
      121 aattacgttc accgtaacaa catggccacc cccgaagttc ccgccgagat gatccgccgg
      181 cctccggccc gggcagtgcg ctgggtcgaa gccgcggtcg gacggggcgc ccgggtggtg
      241 cgcacgcgac ggctccgcaa cgcgtgggcc gcggccatgc acgcggtcga cgtcgagcac
      301 gacggccggc ggcacgcgct cgtcttgcgg cgatgggtgc ggcgcgactt tccgcccgat
      361 gtcggcgtcg tcgacaacga agccgcggtg ctctcgctgc tcgcgacgca cgacctctcg
      421 gtgccgcgtc tcgtcgccgc cgaccccgcg ggcaccgaat gcgacgtgcc cgcggtgttg
      481 atgacgcgat tgaccggtcg cgacgaactg agccccgccg acctcgacgg ctggctcgac
      541 ggtctcgcgg cggcccatgc cgcgatccgc accgtcgccg tcccgccggg cgtgctcggc
      601 tactaccggc cgtggggttg ggacaccgtg accgcgccac caccgtggtc gtcgcggccg
      661 gacgcgtggg cacgcgcgat cgagatcgtc cactccggtg tccctgcgac cgagctcgtg
      721 ttgtgccacc gcgacttcca tcccggcaac gtgctgtggt cgcgcggccg ggtctcgggc
      781 atcgtcgact ggtcgcacgc gtgccgaggc gcgcccgccg ccgacatcgc gcactgccgc
      841 atgaacctga acacgctgtt cgacctcgat gccgccgacg cgttcacggc gcgctgcgca
      901 ccggtcgagc atctcccctg gttcgatctc gccgacgtcg tgagcctcgg cgaatcgccg
      961 ccggaggtat ggcggttcca cgacgcgggc cgcaccgacc tcaccgtgcg ctcgatgatc
     1021 gccgcgaccg acgcgttcgt cgtgcagggc gtcgagcgtt acgacggcgt gtaggcgggc
     1081 gacctggagg catacttcgg ccggacggtc ggtacagaag ccgtcgtggg caggcgcgat
     1141 cgagccttca tcgtgggtgc gttcgcgctc gtcatcggcc tcactgcggt cctgagctgg
     1201 cggctgccgt atgccggcga cgaggccagc cacctcgtga tggtgcgcca tcacgcgcat
     1261 cacctcgacc tcgtctcgtg ggacgagatg caatacggcg gcgagcgtgg acactcctac
     1321 ttcctcttct cgccgctgcc gtacgcggtg tacgtgccct tcgcgttgct cgacgagcac
     1381 gtcggtgacc tgcccggcgc ggtgcgaccc gaccgcgagc tcacacgact cggcggcggc
     1441 atcgtgctcg cggccgcgca actcgcgctg accctcgcgc tcgtgcggcg gatgatccgg
     1501 tcgtcggatc agacgaccgt gaccgccatc gcgctcgccg ccaacctcct gccgcagctg
     1561 cggtacatcc acgcgtatcc caataccgac ggcgtcacga ttctcgccgg cactgcgtgt
     1621 ttctgggcgg cgctgcgcgt cctgcaatcc gaacgcgtcg gcctgcgtga cgcagcactc
     1681 gtcggcgtgc tcatcggcgt cacgatgcac gtgcggttcc aggcattcct cgcggccgcg
     1741 gtcctcctcg ccgcgttcgc agtccgcgcg ctgcaacagt ccgtgtcact ccggccgcgg
     1801 ttgcgcctgc tgggcgtcgc ggtcgcgatc ccggtgttgt tggcgggcag cttccatctc
     1861 gcgatctacg acgagctcga caacggtcac ctcctgccga cggtcgacaa cgaacaactc
     1921 aacgactcca cgtactcggg tgtgctcgtc gccgagcccg cggcgaagtc gctcgtcagc
     1981 cgccgcgtga accagatccc cgaggtctgg atgggcgtgt gggcct
//
LOCUS       DASUGA010000579         2188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1276191, whole genome shotgun sequence.
ACCESSION   DASUGA010000579 DASUGA010000000
VERSION     DASUGA010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276191"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="VFX21_11420"
     CDS             complement(<1..1065)
                     /locus_tag="VFX21_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX5096619.1"
                     /translation="MVEADVLRATLEHALRNGGDFAEVFVEDRRSSNARFDDGRIEEL
                     VSSRERGAGIRVVRGDTTGFAHTADLSPAGLAAAAAAASGAARGGGGGTRTIALAAQT
                     TPKTHEVDVLPETVDKSRKVELLRRADAAARAEGNAISSVTAGYADATRRFQVANSDG
                     LLAGDEQVRTRMSVQCVATGDTGMQTGYEAPGRSIGFEFFDEIDPEDVARSAARRAIT
                     LLDAVPAPSGKVPVVLRRGAGGVLFHEACGHGLEADHILKDASVFTGMIGEQVASPLV
                     TIVDDGTYAREWGTLSIDDEGKPAQRNVLIENGVLTDYMWDLLRARKEGRPSSGNGRR
                     QTYRHLPMVRMTNTFLLEGED"
     gene            1141..1527
                     /locus_tag="VFX21_11425"
     CDS             1141..1527
                     /locus_tag="VFX21_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEX5096620.1"
                     /translation="MPVKPGLAASVTLLVEERDTAVALGSGDVPVLGTPRVVALAEQA
                     SVEAVADELPEGATSVGYAVQLSHMAPTPVGQKVTAEATLETVEGRRLTFRVTVNDAR
                     GLVAAGRLTRVVVERTRFIERAHGES"
     gene            1536..>2188
                     /locus_tag="VFX21_11430"
     CDS             1536..>2188
                     /locus_tag="VFX21_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003938600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5096621.1"
                     /translation="MGDLLVERGDGVARLTLNRPEKKNALSIALRDEISVALDAIAAD
                     GSVKCVVFTGAGNVFCAGFDLSEFPRSFDDQEYSDLLWASSDRYHHAVLECPVPTIAA
                     VNGPALAGGFDLAVLCDIRIATTNAGFAHPEFTFGDVVYGPLHDLVGGAVARDLCLTG
                     RALTAEQALRVNLVSAVVAEAALGAAVDEIVARIVQAPRELLMRTKAKANARARIAT"
BASE COUNT          305 a          781 c          756 g          346 t
ORIGIN      
        1 gtcttcgccc tcgagcaaga acgtgttggt catgcgcacc atcggcaggt ggcgatacgt
       61 ctgccggcgc ccgttgccgc tgctcgggcg accctccttg cgggcgcgca gcaggtccca
      121 catgtagtcg gtgagcacac cgttctcgat gagcacgttg cgctgcgcgg gcttgccttc
      181 gtcgtcgatg ctcagcgtgc cccactcgcg cgcgtacgtg ccgtcgtcga cgatcgtgac
      241 gagcggcgac gcgacctgct cgccgatcat gccggtgaac acggatgcgt ccttgaggat
      301 gtggtcggcc tcgagcccgt gcccgcacgc ctcgtggaag agcacgccac cggcgccgcg
      361 ccgcaacacg acgggcacct tccccgacgg cgcaggcact gcatccagca acgtgatcgc
      421 gcggcgcgcg gccgaacggg cgacgtcttc ggggtcgatc tcgtcgaaga actcgaaccc
      481 gatgctgcgg cccggcgctt cgtagccggt ctgcatgccg gtgtcgccgg tcgcgacgca
      541 ctgcacggac atgcgggtgc gcacctgctc gtcgccggcc agcaatccgt cggagttcgc
      601 gacctggaag cggcgggtcg cgtccgcgta gccggcggtc accgacgaga tcgcgttgcc
      661 ctcggcgcgg gccgcggcat cggcccggcg caacagctcg accttgcggc tcttgtcgac
      721 cgtctccggc aggacgtcga cctcgtgtgt cttcggcgtc gtttgcgccg ccaacgcgat
      781 cgtgcgggtg ccgccgcctc cgccacgcgc cgcgcccgag gccgccgcgg ccgcggccgc
      841 gaggccggcc ggcgacaggt cggcagtgtg cgcgaatccc gtcgtgtcac cgcgcacgac
      901 gcgaatgccc gctccccgct cgcggctgga cacgagctcc tcgatgcgac cgtcgtcgaa
      961 tcgtgcgttc gacgagcgcc ggtcctcgac gaaaacctcc gcgaaatcgc ccccgttgcg
     1021 caatgcatgt tcgagcgtgg cccgcaggac gtctgcttca accattctca gggcgccttt
     1081 tgtcgcggtc ttgacgatgc tgaagtgccc ggatcgggcc gggctgccta tcgtacgcac
     1141 atgcccgtga agcctggtct cgctgcgtcg gtgaccctcc tcgtcgagga gcgcgacacc
     1201 gcagtggcgc tgggcagcgg cgacgtcccc gtgctcggga ccccgcgcgt ggtcgcgctc
     1261 gcggaacagg ccagtgtcga agccgtcgcc gacgagctgc ccgaaggtgc gacgagcgtc
     1321 ggctacgcgg tgcagttgag ccacatggcg ccgacaccgg tgggccaaaa ggtcaccgcg
     1381 gaagcgacgc tcgagaccgt cgaaggtcgg cgcctgacgt tccgggtcac cgtcaacgac
     1441 gcgcgcggct tggtcgcggc cggacggttg acgcgcgtcg tcgtcgaacg cactcgcttc
     1501 atcgaacgcg cccacggcga gagctgagcc gccccgtggg cgaccttctc gtcgagcggg
     1561 gcgacggggt cgcgcgcctc acgctcaacc ggccggagaa gaagaacgcg ctgtcgatcg
     1621 cgttgcgcga cgagatcagc gtcgcgctcg acgcgatcgc cgccgacggg tcagtgaagt
     1681 gtgtcgtgtt cacgggcgcg ggcaatgtgt tctgcgccgg cttcgacctc tcggagttcc
     1741 cccgctcgtt cgacgaccag gagtactccg acctgttgtg ggcatcgagc gaccggtacc
     1801 accacgcggt gttggagtgc ccggtcccga cgatcgcggc ggtgaacggt cccgccttgg
     1861 ccggcggctt cgacctcgcc gtgttgtgcg acattcgcat cgcgacgacg aatgcgggct
     1921 tcgcgcaccc ggagttcacc ttcggcgacg tcgtctacgg cccgctgcac gacttggtcg
     1981 gcggcgccgt cgcccgcgac ctgtgcctca ccggacgcgc gttgacggcc gagcaggcgt
     2041 tgcgggtgaa cctcgtcagc gcggtcgtcg cagaagccgc gctgggggct gcggtcgacg
     2101 agatcgtcgc ccgcatcgtg caggcacccc gcgagctcct gatgcgcacc aaggcgaaag
     2161 ccaacgcccg cgcccgcatc gcaaccga
//
LOCUS       DASUGA010000580         3074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_581336, whole genome shotgun sequence.
ACCESSION   DASUGA010000580 DASUGA010000000
VERSION     DASUGA010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581336"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="VFX21_11435"
     CDS             <1..948
                     /locus_tag="VFX21_11435"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5096622.1"
                     /translation="TLARRFTVSDHHYSSLLGDTFVNRQYLHAATSEGRKDPPKQLDV
                     GVYTGDTIWDRLATAGVSARYYYTDLPFLALWGDRFADRIGAVDQYFDDAQRGALPHV
                     VMVDPGFGGADRSDNHPHGDIRMAQRFLHSVFSAFAQSPQWERGLFVVTYDEWGGFFD
                     HVRPPVFPDDRASADDEQNFGQAGFRVPTILASPYARRGFVDTAPYDHTSILRFVEWR
                     FLGAPASGTRAPNGRWYLTKRDRAAQNIGASLVTDADPELDFDLDEPLPALTDDCDSA
                     VAPPATEERGPSDVPHSPAFDELISNAFPPATERPWETA"
     gene            1004..2167
                     /locus_tag="VFX21_11440"
     CDS             1004..2167
                     /locus_tag="VFX21_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5096623.1"
                     /translation="MFDLTPELAELQATVRKLAQEKVAPRAREIDTTGEYPQDLFELF
                     RDTGLLGLVIPEEYGGSGAGILGLTIAMEEVAKYSNTAALMLLLTRLPTGPVLIAGSE
                     EQKQEYVRGVATGELRAGFGLSEPQAGSDVVGMRTKAVRDGDDWVLSGTKCWMSGVVQ
                     ADWYCVFAKTGPADSRKHDDISCFIVERSAPGVSVGRTDEKMGVRGVDTGELILDEVR
                     VPSANVVGEVGGFRLAMLGLNSMRPIVAARGIGLAEGALMYAVDYVKERAAFGKTIAD
                     MQGIQWKIAEMATEIEAARLLTYRAAQMADEGKYTREFVPFLSMAKYYATEVAVKVSG
                     EALQLLGAAGYMKDHLTELYYRDARQLTIVEGTSQIQLGLIARGVLDRDLWWD"
     gene            2181..>3074
                     /gene="trpD"
                     /locus_tag="VFX21_11445"
     CDS             2181..>3074
                     /gene="trpD"
                     /locus_tag="VFX21_11445"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX5096624.1"
                     /translation="MLTPVLARVLRGETLTDGEIGAAMAEILEGRVTDIQAAGFIVAL
                     RTKGETVDELAALVRAIRAYDVLVDVAPGAIDTCGTGGDGACTVNVSTMAALVAAGAG
                     ARVVKHGNRAASSQCGSADVLEALGVTIELEPEDVARCVEAAGVGFCFAPKYHPAYRF
                     LGPARRELGVPTTFNFLGPLLNPGQVTRQSIGVSDEAMAARMLATLRALGAEHALVFH
                     GDDGLDELTTTTTNTVHELVRGEVKTYRVDPVDLGLERVDAEALRGGSADENANALRA
                     VLAGEKGAHRDITLLNAAAALI"
BASE COUNT          476 a         1049 c         1072 g          477 t
ORIGIN      
        1 acgctcgcgc gccgcttcac cgtctccgac catcactact cgtcgttgct cggcgacacg
       61 ttcgtgaacc gtcagtacct gcatgccgcg acgtcggagg gacgcaagga cccgcccaag
      121 caactcgacg tcggcgtgta caccggcgac acgatctggg atcgcctcgc gaccgcgggc
      181 gtgtcggcgc gctactacta cacggatctg ccgttcctcg cgttgtgggg tgaccgtttc
      241 gccgaccgca tcggggcggt cgaccagtat ttcgacgacg cgcagcgcgg cgcgctgccg
      301 cacgtcgtca tggtggatcc gggcttcggc ggcgcggatc gttccgacaa ccacccgcac
      361 ggcgacattc gcatggcgca acgcttcttg cactcggtgt tcagtgcgtt cgcgcagtcg
      421 ccgcagtggg agcggggcct gttcgtcgtg acctacgacg agtggggtgg gttcttcgac
      481 cacgtcagac cgccggtgtt ccccgacgac cgcgcgtcgg ccgacgacga gcagaacttc
      541 ggtcaggccg gcttccgagt gccgacgatc ctcgcgtcgc cgtacgcgcg ccgcgggttc
      601 gtcgacactg cgccgtacga ccacacgtcg atcctgcgct tcgtggagtg gcggttcctc
      661 ggcgcgccgg catcggggac gcgcgcaccg aacggccgct ggtatctgac gaagcgagat
      721 cgcgccgcgc agaacatcgg cgcgtcgttg gtcaccgatg ccgatccgga gctcgacttc
      781 gacctcgacg aaccgctgcc ggcgttgacc gacgactgcg actccgctgt ggccccgccc
      841 gcgaccgaag agcgaggtcc gtccgacgtg ccgcacagtc ccgcgttcga cgagctgatc
      901 tcgaacgcgt tcccacctgc gaccgaacgc ccctgggaga ccgcgtgata ccggccgcgc
      961 acgaaccgga ccgttttcgg gcgctggcct aggctgcacg ccgatgttcg acctgacgcc
     1021 cgagctggcg gagctccagg ccaccgtccg caagctggcg caggagaagg tggcgccccg
     1081 ggcccgggag atcgacacca cgggcgagta cccccaggat ctcttcgagc tgttccgcga
     1141 caccggcctg ctcgggctcg tgatccccga agagtacggc gggtcgggcg ccggcatcct
     1201 cggattgacg atcgcgatgg aagaggtcgc gaagtactcg aacacggccg cgctgatgct
     1261 gttgttgacg cgcctgccga ccgggcccgt gctcatcgcc ggcagtgagg agcagaagca
     1321 ggagtacgtg cgcggcgtcg cgaccggcga gctgcgcgcc ggcttcgggc tctcggagcc
     1381 gcaggcgggc agcgatgtcg tggggatgcg caccaaggcc gtgcgcgacg gcgacgactg
     1441 ggtgctgtcg ggcacgaagt gctggatgtc gggtgtcgtg caggccgact ggtactgcgt
     1501 gttcgcgaag accggtccgg ccgactcccg caagcacgac gacatctcgt gcttcatcgt
     1561 cgaacgctcg gcgccgggcg tcagtgtcgg tcgcaccgac gagaagatgg gtgtgcgcgg
     1621 cgtcgacacc ggcgagttga tcctcgacga ggtgcgtgtc ccgagcgcga acgtcgtcgg
     1681 tgaggtcggc ggcttccggc tggcgatgct cggcctcaac tcgatgcgcc ccatcgtcgc
     1741 cgcgcgcggc atcggtctcg ccgaaggcgc actgatgtac gccgtcgact acgtgaaaga
     1801 gcgcgccgcg ttcggcaaga ccatcgccga catgcagggc atccagtgga agatcgcgga
     1861 gatggcgacc gagatcgaag cggcccgcct gctcacctac cgcgccgccc agatggccga
     1921 cgaaggcaag tacacgcggg agttcgtgcc attcctctcc atggccaagt actacgcgac
     1981 cgaagtcgcg gtgaaggtct ccggtgaagc gctgcagctg ctcggcgcgg cgggctacat
     2041 gaaagaccac ctgaccgagc tctactaccg cgacgcgcgc cagctcacga tcgtcgaagg
     2101 cacctcgcag atccagctcg gcctcatcgc tcgcggcgtg ctcgatcgcg acctgtggtg
     2161 ggattgaagc cttgaacgag gtgctcaccc ccgtcctcgc ccgcgtgttg cggggcgaga
     2221 cgttgaccga tggcgagatc ggcgcagcga tggccgagat cctcgaaggg cgcgtcaccg
     2281 acattcaggc cgcgggcttc atcgttgcgc tgcgcaccaa gggtgagacc gtcgacgagc
     2341 tcgccgccct cgtgcgcgcg atccgcgcct acgacgtcct cgtcgacgtc gcacccggcg
     2401 cgatcgacac gtgcggcacc ggcggcgacg gcgcgtgcac cgtcaacgtg tcgacgatgg
     2461 cggcgctggt cgccgcgggc gcgggtgcgc gagtggtcaa gcacggcaac cgcgcggcgt
     2521 cgtcgcagtg cggctcggcc gacgtgctcg aggcgctcgg cgtgaccatc gagctcgaac
     2581 cggaggacgt cgcccgctgc gtcgaggccg ccggcgtggg gttctgcttc gcgcccaagt
     2641 accaccccgc gtaccggttc ctcgggcctg cccgccggga gctgggggta ccgaccacct
     2701 tcaacttctt ggggccattg ctgaatcccg gtcaagtcac ccgccagtcg attggcgtct
     2761 ccgacgaggc gatggcggcc agaatgctgg cgacgctgcg cgcactcggc gcggagcacg
     2821 cgttggtgtt ccacggtgac gacgggctgg acgagttgac cacgaccacg acgaacacgg
     2881 tgcacgagct cgtccgcggt gaggtgaaga cgtaccgcgt cgaccccgtc gatctcggtc
     2941 tggagcgagt cgacgcggag gcgttgcggg gcggctcggc ggacgagaac gcaaatgccc
     3001 tgcgcgccgt gctggccggc gagaagggcg cgcaccgcga catcaccctc ttgaacgcgg
     3061 cggccgcgct gatc
//
LOCUS       DASUGA010000581         2806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_461150, whole genome shotgun sequence.
ACCESSION   DASUGA010000581 DASUGA010000000
VERSION     DASUGA010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461150"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VFX21_11450"
     CDS             <1..967
                     /locus_tag="VFX21_11450"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5096625.1"
                     /translation="VVPVFSTTKGLAGLAMAHAHARGLYDHDERVAKYWPEFAQEGKD
                     AITVRQLLSHQAGLPLVEGEYTLDAALDWDTVVEQLACQAPAWEPGTKHGYHMRTFGW
                     LVGELIRRVSGSSVGQFLRDEIATPLGLDFWIGLPEEIEPRVARLVPPKSDMRAALEP
                     YRDKLLLARVFENPGGHFNYDEMWNTRALHACELPSSNGIGDARSLARLYAATIGDVD
                     GVRILRDDTVATATQEQARGPDAVIMVESRFGFGFMLGASFGARNPDHVFGHAGAGGS
                     LAFADPRAGIGFGYVMNDLRFDPKGDPRSETLVAACYESLAASAG"
     gene            complement(937..>2806)
                     /locus_tag="VFX21_11455"
     CDS             complement(937..>2806)
                     /locus_tag="VFX21_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096626.1"
                     /translation="PELAPGALGDVGGTYALAIDERIVTFSPASGALTYARGAPAESL
                     AIVNANHGNVLARSPSGTFLFDAQGLRAEIEDQNVFPGFADDQWWRPDEDKLLPIDDS
                     EPVQLPAATRARAELRGGFLLQSMDSDALLAWTPGGATRPLAEAEGVALVAAHPDRIA
                     WSGRCPGLSCGLHVTDVATARTVDLPIDAAFTQQSGIARGRFSGDGRLLGVVFAPDVR
                     GMRDFALVDLADGNVLARRMIGNGTFTNSPFGVPFDFNPDATRLVLADASRGARLLAI
                     DTTTGEEVASTPNLPPVPSLASFDRVMTEATTPLFTGPAVSGLPSGLVLAAIDGERGD
                     LTIADIDTGETRTIDVDGGGPVLGSSWGPALKALDGGFVAATNGKALWVPDTGDPVDL
                     GPAVYVLPGDDTTGAWIVTRDESSADYALRRVDGRTGALGDVTIEVHAQPMYATATRF
                     VRSSEGTFARPGGVELLDIATGDTTFVDLGTQYPNLTAIVGDEVAWFGNDCYQSSACT
                     LQLTNIVTKRTRTLEVDTDYAQAAGGYGALYVRRADGIARVDLEDLSEETVPDSSTAN
                     FFSTTASGAVVFYTPGSVSMWQPGWETSRPLKVGNFVDIGMVAVRQRQPADAASDS"
BASE COUNT          418 a          955 c         1019 g          414 t
ORIGIN      
        1 ggtcgtaccg gtgttctcga ccaccaaggg cctggccggg ctcgcgatgg cgcacgcgca
       61 cgcccgcggt ctctacgacc acgacgagcg cgtcgcgaag tactggccgg agttcgcgca
      121 ggagggcaag gacgcgatca cggtgcgcca gctgctcagc caccaggccg gcctgccgct
      181 cgtggaaggg gagtacacac tcgacgccgc gctcgactgg gacaccgtcg tcgagcagct
      241 cgcgtgccaa gcgcccgcat gggaaccggg tacgaagcac ggctatcaca tgcgcacctt
      301 cggctggctc gtcggcgagc tgatccggcg ggtcagcggc tcgagtgtcg ggcagttcct
      361 gcgcgacgag atcgcgacgc cgctcgggct cgacttctgg atcggtctcc cggaggagat
      421 cgaaccgcgc gtcgcccggc tcgtgccgcc gaagtccgac atgcgcgccg cgctcgagcc
      481 gtaccgcgac aagctcctcc ttgcgcgcgt cttcgagaat cccggcggtc acttcaacta
      541 cgacgagatg tggaacacac gcgcgttgca cgcgtgtgag ctcccgtcct cgaacggtat
      601 cggcgatgcg cgttcgctcg cgcgcctcta cgcggcgacg atcggcgacg tcgacggcgt
      661 gcgcatcctc cgcgacgaca cggtggcgac cgcgacgcag gagcaagcgc gcggtccgga
      721 tgcggtgatc atggtcgaga gtcggttcgg gttcgggttc atgctcggcg ccagcttcgg
      781 cgcgcggaac cccgaccacg tgttcggcca cgcgggcgcg ggcggctcgc ttgcgttcgc
      841 cgatccgcgc gcgggcatcg gcttcggcta cgtgatgaac gacctgcgct tcgacccgaa
      901 gggcgatccg cgcagcgaga cgctggtcgc cgcctgctac gagtcgctcg cggcgtccgc
      961 cggctgacgc tggcgcaccg cgaccatgcc gatgtccacg aagttgccga ccttcaacgg
     1021 gcggctggtc tcccagccgg gctgccacat cgagaccgac ccgggcgtgt agaagaccac
     1081 ggcgccgctc gcagtcgtgc tgaagaagtt cgccgtcgac gagtccggca cggtttcctc
     1141 actgagatcc tcgaggtcga cgcgagcgat cccatcggcg cggcggacgt acagcgcgcc
     1201 gtagccgccc gccgcctggg cgtagtcggt gtcgacctcg agcgtgcgcg ttcgcttcgt
     1261 cacgatattc gtgagttgca gcgtgcacgc ggaggactgg tagcagtcgt tgccgaacca
     1321 cgcgacctcg tcgccgacga tcgcggtgag attcgggtac tgcgtgccga ggtcgacgaa
     1381 ggtcgtgtcg ccggtagcga tgtcgagcag ctcgacccca ccgggccgcg cgaacgtgcc
     1441 ttcggacgac cgcacgaacc gggtggcggt ggcgtacatc ggttgcgcgt gaacctcgat
     1501 ggtcacgtcg ccgagtgcac ccgtacgacc gtcgacgcgg cgcagtgcgt aatcggcgct
     1561 gctctcgtcg cgagtgacga tccaggcgcc ggtggtgtcg tcgcccggga ggacgtacac
     1621 ggccggaccg agatccacgg ggtcgccggt atcggggacc cagagcgctt tgccgttggt
     1681 cgcggcgacg aagccgccgt cgagcgcctt cagcgcggga ccccagctgc tgccgaggac
     1741 cgggccgccg ccgtcgacgt cgatcgtgcg cgtctcgccg gtgtcgatgt ccgcgatcgt
     1801 gaggtccccg cgctcgccgt cgatcgcggc gagcacgaga cccgacggca ggccgctgac
     1861 cgccggcccg gtgaagagcg gtgtggtcgc ttcggtcatg acccggtcga acgacgccag
     1921 cgagggtacc ggggggaggt tgggtgtgct cgcgacttcc tcgccggtcg tcgtatcgat
     1981 cgcgagcagg cgggcacccc tcgacgcgtc ggcgaggacg agccgcgtcg cgtcggggtt
     2041 gaagtcgaac ggcacgccga acggcgagtt cgtgaacgtg ccgttgccga tcatccggcg
     2101 cgcgagcacg ttgccgtcgg ccaggtcgac aagggcgaag tcgcgcatgc cccggacgtc
     2161 gggcgcgaag acgacgccga gcagccggcc gtcgccggag aaccggccgc gcgcgatccc
     2221 ggattgctgt gtgaacgcgg cgtcgatcgg gagatcgacc gtgcgcgcag tcgcgacgtc
     2281 ggtcacgtgg aggccgcaac tgaggcccgg gcaccgcccc gaccacgcga tgcggtcggg
     2341 gtgcgcggcc accagtgcga cgccctcggc ctcggcgagc gggcgcgtcg caccacctgg
     2401 cgtccatgcg agcaacgcat cggagtccat ggactggagg aggaagcccc cgcgcagttc
     2461 cgcgcgcgcg cgggtggccg ccgggagttg caccggttca ctgtcgtcga tgggcagcaa
     2521 cttgtcttcg tcggggcgcc accactggtc gtccgcgaag ccggggaaga cgttctggtc
     2581 ctcgatctcg gcgcgcagac cctgggcgtc gaagagaaac gttcccgacg gcgaacgcgc
     2641 gaggacgttg ccatggttcg cgttgacgat ggccagcgac tcggccggcg cgccgcgcgc
     2701 atacgtgagc gcgcccgagg ccggggagaa ggtcacaatc cgttcgtcga tcgcgagcgc
     2761 gtaggtgccg ccgacgtcgc caagtgcacc aggcgccaac tcgggc
//
LOCUS       DASUGA010000582         2647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_940523, whole genome shotgun sequence.
ACCESSION   DASUGA010000582 DASUGA010000000
VERSION     DASUGA010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940523"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="VFX21_11460"
     CDS             complement(<1..1259)
                     /locus_tag="VFX21_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096627.1"
                     /translation="MSWNPPEPAPAKRSPAVPIVIAVVVTALIAGGLTWFVMRDDDND
                     NAASSTTTTRRETTTPDTSEGGNEDDPLSSLDDLLGGDAGDLGGLLGGNIDAMSKCVG
                     ADDMSSLLGGGDPLPDTDPRTQYQEIAQRVEEERGLTFDHVPEPVFVDSQEMQSRVER
                     QIEADYPADAAQADEELYKALGVIDDDTNLLDEYSKFVGSQVAGYYDPDTGELVVLGD
                     DTEPFDALEMTTIAHELEHALADQKLGFPIEDTPVAADEDKQMAGLALIEGDATLTMT
                     RFQLSAVDLMSLLGSLGGAGLDAQTKALEDAPRYFASQLMFPYTEGLGFVCTLEADGG
                     WPAVDDAYENAPTTTAQIMFPDRYTSGEGAVAVPDPQSPGDGWEHVRTTTFGAAELMF
                     LLQDAFVNEPRDRAAAWAGGKVSQWRNG"
     gene            complement(1240..2337)
                     /locus_tag="VFX21_11465"
     CDS             complement(1240..2337)
                     /locus_tag="VFX21_11465"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX5096628.1"
                     /translation="MTQVTVLGGGQLGRMLGLAGLPLGIQFQFLDPVDGAPAEIAGPV
                     VVGALDDLAAASEAAVGADVVTFEWEGVPAATAEHIAAKVPVYPPPRALDVAQDRVTE
                     KQTCNDLGIATAPWRAVDDRAGLTQAVSEIGLPAVLKTRRGGYDGKGQAVLRDEADLD
                     RSWRAVGEVPSILEGFVPFTRELSVLAVRGRDGTAACWPVNENEHRDGVLRVTRAPAR
                     GLTPELQARAEACIVPLLADLDYVGVLCVELFDVDGELLANEIAPRVHNSGHWTIEGA
                     QASQFENHVRAVLGWPLGSTAARGVNAMVNCIGAMPDRDTILAIPGAHLHDYGKTPRA
                     GRKVGHVTITATTDDEREQLLGRVCNVVESA"
BASE COUNT          362 a          915 c          927 g          443 t
ORIGIN      
        1 ccgttgcgcc actggctgac cttgccgccc gcccatgctg cggcgcggtc gcgtggttcg
       61 ttgacgaacg cgtcctgcaa caagaacatg agctcggctg cgccgaacgt cgtggtgcgg
      121 acgtgctccc acccgtcgcc cgggctctgc gggtcgggta ctgcgaccgc gccttcaccg
      181 gaggtatagc ggtcggggaa catgatctgc gccgtcgtgg tcggcgcgtt ctcgtacgcg
      241 tcgtcgaccg caggccagcc gccgtcggct tcgagtgtgc agacgaagcc cagcccttcg
      301 gtgtagggga acatcagttg cgacgcgaag taccgcggtg cgtcctcgag tgctttcgtc
      361 tgcgcgtcga gcccggcgcc acccagcgat ccgagcagtg acatcaagtc gacggcggac
      421 agctggaacc gggtcatcgt cagcgtcgcg tcaccttcga tgagtgcgag cccggccatc
      481 tgcttgtctt cgtcggcggc gaccggcgtg tcctcgatgg ggaagccgag cttctggtcg
      541 gcgagcgcgt gctccagttc gtgcgcgatc gtcgtcatct cgagtgcgtc gaacggttcg
      601 gtgtcgtcgc cgagcacgac gagctcgccc gtatcagggt cgtagtaccc ggcaacctgc
      661 gaaccgacga acttcgagta ctcgtcgagg agattggtgt cgtcgtcgat gacacccagc
      721 gccttgtaga gctcctcgtc ggcctgcgcg gcgtcggcgg ggtagtcggc ctcgatctgc
      781 cgctcgacgc gcgactgcat ctcctgcgag tcgacgaaca cgggctcggg gacgtggtcg
      841 aacgtgagcc cgcgctcttc ttcgacgcgc tgcgcgatct cctggtattg cgtgcgcggg
      901 tcggtgtcgg ggagcgggtc gccgccgccg agcagcgacg acatgtcgtc ggcgccgacg
      961 cacttcgaca tcgcatcgat gttgccaccg agcaacccgc cgaggtcacc ggcatcgccg
     1021 ccgaggaggt cgtcgagcga gctcaacgga tcgtcttcgt tgccgccctc ggatgtgtcg
     1081 ggcgtcgtcg tctcgcggcg cgtcgtcgtc gtgctcgacg ccgcgttgtc gttgtcgtcg
     1141 tcgcgcatga cgaaccaggt cagcccgccg gcgatgagcg cggtgaccac gaccgcgatg
     1201 acgatcggga cggcgggcga ccgtttggcc ggcgccggtt caggcggatt ccacgacatt
     1261 gcacacccgt cctagtagtt gctcgcgttc gtcatccgtg gtggccgtga tcgtcacgtg
     1321 gccgaccttg cggcccgcgc gcggtgtctt gccgtagtcg tgcaggtgcg cacccgggat
     1381 cgcgaggatc gtgtcgcggt cgggcatcgc gccgatgcag ttgaccatcg cgttcacgcc
     1441 gcgcgctgcc gtcgaaccga gcggccagcc gagcacggcg cgcacgtggt tctcgaactg
     1501 actcgcctgc gcgccttcga tcgtccagtg cccgctgttg tgcacgcgcg gggcgatctc
     1561 gttcgcgagg agctcgccgt cgacgtcgaa gagctccacg cacaacaccc cgacgtagtc
     1621 gaggtccgcc agcagcggca caatgcacgc ctcggcgcgc gcttgcagct cgggcgtgag
     1681 cccgcgcgcc ggcgcgcgcg tcacacgcag gacgccgtcg cggtgctcgt tctcgttcac
     1741 cggccagcag gccgccgtgc cgtcgcgccc gcgcacggcg aggacggaaa gctcgcgtgt
     1801 gaacgggacg aacccttcga ggatgctcgg gacctcgccg accgcgcgcc atgagcggtc
     1861 gaggtcggcc tcgtcgcgca ggacggcctg gcccttcccg tcgtagccgc cgcggcgtgt
     1921 cttgagcacc gcgggcagac cgatctcgga caccgcctgc gtgagcccgg cgcggtcgtc
     1981 gaccgcgcgc cacggcgccg tcgcgatgcc gaggtcgttg cacgtctgct tctcggtgac
     2041 tcggtcttgc gcgacgtcga gcgcgcgcgg cggcgggtac acgggcactt tggcggcgat
     2101 gtgctcggca gttgcggccg gcacgccttc ccactcgaag gtcacgacgt cggcaccgac
     2161 cgcggcttcg ctcgccgccg cgagatcgtc gagcgcgccg acgacgacgg gtccggcgat
     2221 ctccgcgggc gcgccgtcga cggggtcgag gaactggaac tggattccga gcggcaggcc
     2281 ggcgagcccg agcatgcgcc cgagctgccc gccgcccagc accgtcacct gcgtcatcag
     2341 gtgaaccaac gtaggtgatc ggtgccgggt tcccgccgcg tggttgctcg gcaccgaccc
     2401 gcgcctggac ccggcggatg caggccggca agcgagagtc gcgaaaagaa tctcggccgg
     2461 acggatctgg cggttcgatc gtggacgggt cccatcccgc ctgggtctct cacccctcgc
     2521 cacgacgatc tcacgctcca cggtcgttgc aatcgacccc gcgctctggt ctggtggaga
     2581 cgaccgatgg gagccgccgg cgtgggccag acaacggatc cggagcggag ctggacgttc
     2641 ctcacca
//
LOCUS       DASUGA010000583         2877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_713165, whole genome shotgun sequence.
ACCESSION   DASUGA010000583 DASUGA010000000
VERSION     DASUGA010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713165"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFX21_11470"
     CDS             complement(<1..234)
                     /locus_tag="VFX21_11470"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX5096629.1"
                     /translation="MRAGPEFAAFYTAEHPRVLGVLCAVAGDRDAAIEATDEAFVRAL
                     ERWHRVGALVSPAGWTYRTALNALRRAKRRGAME"
     gene            complement(342..662)
                     /gene="erpA"
                     /locus_tag="VFX21_11475"
     CDS             complement(342..662)
                     /gene="erpA"
                     /locus_tag="VFX21_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX5096630.1"
                     /translation="MIALTDTATSKVKELIEAEGQPELFLRVAVRPGGCSGLSYDMFF
                     DTERADDDVEQSYGEIKVVVDPASAQYLEGASLDYKDGLQGAGFTINNPNAQRTCGCG
                     QSFS"
     gene            complement(683..1345)
                     /locus_tag="VFX21_11480"
     CDS             complement(683..1345)
                     /locus_tag="VFX21_11480"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5096631.1"
                     /translation="MDLLAVLKALGDETRFSMYRELASSPRPVTAHELADRLDLHHNT
                     VRIHLERLREAGLVEVVPVHRGTVGRPQHVYSLAAGAPGLGFDPPSYTLLAGLLAALA
                     ERVGADADEATAIGRSWGSETGKRTRSRSCVKALSAELERLGFDPDLDDDGDLATISF
                     LHCPFRELAEAYPELVCNLHRGICEGVVGTVGGGSVDDFATLYDHDPCRVTVAVGYPE
                     EA"
     gene            1407..2675
                     /locus_tag="VFX21_11485"
     CDS             1407..2675
                     /locus_tag="VFX21_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018811464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEX5096632.1"
                     /translation="MSFPPSPPTESDIRGILAGVLDPELGASIVDLGMVRDITIGPDA
                     DVTVQIALTTAACPLRGQIATDVSSKVAGLPGVRSVDVTYAEMTQGERSAVMQRARLE
                     AQRKAPPTEVPATTRVIAVGSGKGGVGKSSVTANLAAALAARGLTVGVLDADIWGFSI
                     PRMLGAGGRLEATRTDEGKGKIVPHDIPVGPERSGLGTDDNAPSSGAGPERPFGAGKS
                     GKLRVVSMGLLVDDEDTALMWRGLILAKALEQFLTDVQWGDLDYLLIDMPPGTGDIQM
                     ALSRLLPQAEMLVVTTPAKGAQKVASRVADMARRSYLKVLGIVENMSAFVAPDGTTHE
                     IFGAGGGKRLAALTGAPLVGSIPIEPMVSDGGDRGRPVVLEHPDTPAARAFSEIADAI
                     VTSLLPPVEMAGCTARMFDLVNKLTAADAT"
     gene            2686..>2877
                     /locus_tag="VFX21_11490"
     CDS             2686..>2877
                     /locus_tag="VFX21_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018515178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096633.1"
                     /translation="MTREQRLVFGEVADQYDRARPGYPDAAFDAVVEYAPVRPGDRVL
                     EVGAGTGKATTSMLARGLDV"
BASE COUNT          446 a          998 c          998 g          435 t
ORIGIN      
        1 ctccatcgcg ccgcgccgtt tggcgcggcg caacgcgttc aacgcagtgc gatacgtcca
       61 ccccgccggt gagacgagcg ccccgacccg gtgccagcgt tcgagcgccc ggacgaacgc
      121 ttcgtccgtg gcctcgatcg cggcgtcccg gtcaccggcg acggcgcaca gcacgccgag
      181 cacgcgcggg tgctcggccg tatagaacgc cgcaaactcg ggccccgctc gcataccaac
      241 cccactgagt catgaggacg ccgaaatgtt cacccatcgc ggagaaaaag aaaggacccc
      301 gcccgaaggc ggggtccagg ccttgctcca ggcaccagtc gttacgagaa cgactggccg
      361 cagccgcagg tgcgctgcgc gttcgggttg ttgatggtga acccggcgcc ctggagaccg
      421 tccttgtagt cgagcgacgc accctcgagg tactgggcgc tggccgggtc gacgacgacc
      481 ttgatctcgc cgtagctctg ctcgacgtcg tcgtcggcgc gctcggtgtc gaagaacatg
      541 tcgtagctca ggcccgagca gccgcccggg cggacggcga cgcgcaggaa gagctcgggc
      601 tggccttcgg cctcgatgag ctccttgacc ttgcttgtcg cggtatcagt gagtgcgatc
      661 acgtcttgcc tccaacggga aactaggcct cttcagggta gccgaccgcg accgtgactc
      721 ggcacgggtc atggtcgtac aacgtcgcga agtcgtcgac acttcccccg ccgaccgtac
      781 cgacgacacc ttcgcaaatg ccgcggtgca ggttgcacac cagctccgga taggcctcgg
      841 ccagctcccg gaacgggcag tgcaggaacg agatcgtcgc caggtcgccg tcgtcgtcga
      901 gatcggggtc gaagccgagg cgttcgagct cggccgacag ggccttcacg cacgaccgcg
      961 atcgcgtgcg cttgcccgtt tcggaccccc acgaccggcc gatggcggtc gcctcgtcgg
     1021 cgtcggcgcc gacccgctcg gccaacgccg cgagcagccc ggccaacaag gtgtagctgg
     1081 gcgggtcgaa gccgaggccg ggtgcgcccg ccgccaggga atacacgtgt tgcgggcgac
     1141 cgacggtgcc ccggtgcacc gggacgacct cgaccaggcc ggcttcccgc aggcgctcca
     1201 ggtggatccg caccgtgttg tggtgcaggt cgagccggtc cgccaattcg tgggcggtga
     1261 ccggccgcgg cgacgatgcc agctcgcgat acatcgagaa tcgcgtttcg tcgccgagtg
     1321 ctttcaagac cgccagcagg tccatgacgc cttgacggta tcgacgggtg ggcatatttt
     1381 caaggtccag acgggtttta ttcgctgtga gcttccctcc ctctcctccc accgaatccg
     1441 acattcgggg catcctcgcg ggcgtgctcg accccgagct cggggcgagc atcgtcgacc
     1501 tgggcatggt gcgtgacatc accatcggcc ccgacgccga cgtcaccgtg cagatcgcgc
     1561 tcacgaccgc ggcgtgcccg ttgcgcgggc agatcgcaac cgacgtcagc tccaaggtcg
     1621 cgggcctgcc cggcgtgcgc tcggtggacg tcacgtacgc ggagatgaca caaggggaac
     1681 ggtccgcggt catgcagcgg gcccggctcg aagcgcaacg caaggcgccg cccactgaag
     1741 ttccggcgac gacgcgcgtc atcgcggtcg gcagcggcaa aggcggtgtc ggcaagtcgt
     1801 cggtgaccgc gaacctcgca gccgcactcg cggcgcgcgg cctgaccgtc ggtgtgctcg
     1861 acgcggacat ctggggcttc tcgatcccgc gcatgctcgg cgcgggcgga cggctcgagg
     1921 ccacccgcac cgacgagggc aagggcaaga tcgtcccgca cgacattccg gttggtcctg
     1981 agcgcagcgg tctcggcacc gatgacaacg cgccgagcag cggcgcgggg cccgagcggc
     2041 ccttcggagc gggcaagagc ggcaagctgc gggtcgtctc catgggtttg ctcgtcgacg
     2101 acgaagacac cgcgctgatg tggcgcgggc tcatcctcgc caaggcgctc gagcagttcc
     2161 tcaccgacgt gcagtggggc gacctcgact acttgttgat cgacatgccc cccggcaccg
     2221 gtgacatcca gatggcgttg tcgcgcttgt tgccgcaggc ggagatgctc gtcgtcacga
     2281 cgccggccaa gggcgcacag aaggtcgcgt cgcgtgtggc cgacatggcg cgccggtcgt
     2341 acctgaaggt gctcggcatc gtcgagaaca tgtcggcgtt cgtcgcgccc gacggcacca
     2401 cgcacgagat cttcggcgcg ggtggcggca agcgcctcgc cgcgctgacg ggcgcgccgc
     2461 tcgtcggatc gatcccgatc gagcccatgg tctccgacgg cggcgaccgg ggccggcccg
     2521 tcgtgctgga gcaccccgat actcccgcgg cgcgcgcgtt ctcggagatc gcggacgcga
     2581 tcgtgacctc gctgctgcca ccggtcgaga tggcgggctg cacggcccgc atgttcgatc
     2641 tcgtgaacaa gctcacggcc gccgacgcga cctgattgcg cctgtgtgac gcgcgaacag
     2701 cggctcgtgt tcggcgaggt ggccgaccag tacgaccgcg cccggcccgg ctaccccgac
     2761 gccgcgttcg acgcggtcgt cgagtacgcg ccggtgcggc cgggcgaccg cgtgctggag
     2821 gtcggtgccg gtacgggcaa ggcaacgacg tcgatgctgg cgcgcggtct cgacgtg
//
LOCUS       DASUGA010000584         3113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_192558, whole genome shotgun sequence.
ACCESSION   DASUGA010000584 DASUGA010000000
VERSION     DASUGA010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192558"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VFX21_11495"
     CDS             complement(<1..525)
                     /locus_tag="VFX21_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096634.1"
                     /translation="MLLDDLVSDRTVEPLPAPVVTIEPRRVEPPRGRTNARQRAAELE
                     TVAMHNLRAAEEARKVAEQERQRLEAEASIRVKMEREVAGLRREMERLRESEQLRVEE
                     ARYGAERDARAEVRAEVDALAAEHERVQREMERLRSALDDDRNLMQELTERLREEQQA
                     KMKLRVEADKTFEAK"
     gene            825..1562
                     /locus_tag="VFX21_11500"
     CDS             825..1562
                     /locus_tag="VFX21_11500"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5096635.1"
                     /translation="MRFWEARYGGLLLRELALLGVMLWLYKYVRFLINGRTAEAFENA
                     HRILNWEHWLGLDSEAALQRLVLPHHGLVIGLNRYYVSVHFVGTIAFLVWAFARSHVD
                     YQKLRRVLIATTLTALCIHVMFPLAPPRMMNGFVDTMVRFGPNPYDNGHVTGFANQFA
                     AMPSLHIGWSIIVAYGVITIMRSKWRWLVLAHPIMTSLAVVLTANHYWLDGVVAGAIV
                     AACIFFLADPADVRSRSNRQLVRAGYS"
     gene            complement(1585..2877)
                     /locus_tag="VFX21_11505"
     CDS             complement(1585..2877)
                     /locus_tag="VFX21_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5096636.1"
                     /translation="MQECTAEEAAARFRPVDRLGIPLGPGQPVSLLHALGARDDWERF
                     EIFGALLLDLYALFSKPGVRYLTGFFGPAERLLRDTGANIEFIPADFRRFVTIADDFA
                     PRVMATTATPPDEHGFMSLSLHAGATVRELKRAGQDDERLLIVEANAALPRTLGLPPE
                     HPHALHVDEVDVLVHGDRPPYAMEDAPPTDVERAIAQHAASFAVDGCTLQTGIGAVPS
                     TVVGLLAEHGGGDYGVHSEMFTTGLMELHKAGKVTNRRKGVFNGFSVTTFALGTAELY
                     AWLDGNADVRFLPVEVVNAPHTIARNHEVVSINSAIEVDLYGQVVADRVRGEQYSGIG
                     GHEDFVAASGLQLEDRSLVCLPSTATIGGVTVSRVIASVPAGTTVTTPRHQVDVVVTE
                     YGVAELRGRTTRERAAALADIAHPDFRDELRAAAAGYR"
     gene            complement(2883..>3113)
                     /locus_tag="VFX21_11510"
     CDS             complement(2883..>3113)
                     /locus_tag="VFX21_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HEX5096637.1"
                     /translation="TPHPDAFDWNRLFTASLDHALWFHRPVRADEWLLFELRGAGVAD
                     ARGLAFARVFANDVHVASVAQEALGRGRAQGR"
BASE COUNT          447 a         1109 c         1041 g          516 t
ORIGIN      
        1 ctttgcctcg aacgtcttgt cggcctcgac gcgcagcttc atcttcgcct gctgctcttc
       61 gcgcagccgt tcggtgagct cttgcatcag gttgcggtcg tcgtcgaggg cactccggag
      121 ccgctccatc tcccgctgga cgcgttcgtg ttcggccgcg agtgcgtcga cttccgctcg
      181 aacctccgca cgcgcgtcgc gctcggcgcc gtagcgcgcc tcctcgacgc gcaactgctc
      241 gctctcgcgc aggcgctcca tctcgcgtcg cagtcccgcg acttcgcgct ccatcttcac
      301 gcggatcgac gcctcggcct cgagccgctg gcgctcctgc tcggcgacct tgcgcgcctc
      361 ctccgcggcc cggaggttgt gcatcgcgac ggtttccagc tcggccgcgc gctgcctcgc
      421 gttggtccgg ccgcgcggcg gctcgacgcg gcggggctcg atggtcacca ccggcgccgg
      481 caacggctcg accgtgcggt ccgacaccag gtcgtcgagc agcaacttgc ggggggcggc
      541 tccgccggga gcgggcgggg gcggcgaaga cggagcggcg gccatcacct ctgttgtcgg
      601 cgccgggacg cccggtcctt gaccgcgttt gtgaccgtcg acacagctcc gtctcggccg
      661 ggtgaccagg ccggaccacg acccgcggcc gagtgggccc gctcggcgcg caccgccgcg
      721 gccgcgcccg gggccaaaga caggcgcggg ccggcgccta catgcttgga tattcgttcg
      781 gggtacgggg tcacggtgtg aacgaacaac gacgcaatcc ctgggtgcgt ttttgggagg
      841 ctcgctacgg cggcttgttg ctgcgcgagc tcgcgctgct cggcgtcatg ttgtggctgt
      901 acaagtacgt ccggttcctc atcaacggac gcaccgcgga ggcgttcgag aacgcacacc
      961 gcatcctcaa ctgggagcac tggctcggcc tggactccga agcggcgttg cagcgcctcg
     1021 tgctgccgca ccacggtctc gtcatcggcc tgaaccgcta ctacgtcagc gtccacttcg
     1081 tcggcacgat cgcgttcttg gtctgggcct tcgcccgttc gcacgtcgac taccagaagc
     1141 tacggcgggt cctcatcgcg accacattga ccgcgttgtg catccacgtg atgttcccgc
     1201 tcgcgccgcc gcggatgatg aacggcttcg tcgacacgat ggtgcggttc ggtccgaacc
     1261 cgtacgacaa cggtcacgtc accggctttg cgaaccagtt cgcggcgatg ccgtcgctgc
     1321 acatcggctg gtcgatcatc gtcgcgtacg gcgtgatcac gatcatgcgc agcaagtggc
     1381 gctggctcgt gctcgcacac ccgatcatga cgagcctggc ggtggtgctg accgcgaacc
     1441 actactggct cgacggtgta gttgcgggcg cgatcgtcgc cgcctgcatc ttcttcctcg
     1501 ccgacccggc cgacgtccgg agccgctcca accgccagct cgtccgcgcc ggctacagct
     1561 gatcccgacg ccccgaccgc ttcgctagcg gtatccggcc gcggccgcgc gcagctcgtc
     1621 tcggaagtcg ggatgtgcaa tgtccgcgag tgcggccgct cgttcgcgcg tcgtccggcc
     1681 ccgcagctcg gccacgccgt actcggtgac gacgacgtcg acttggtggc ggggcgtcgt
     1741 caccgtcgtg cccgcgggca ccgatgcgat gacacgcgac acggtgacgc ccccgatcgt
     1801 cgcagtcgac ggcaagcaga cgagcgagcg gtcctcgagc tgcagcccgg aggcggcaac
     1861 gaagtcctcg tgcccgccga tgcccgagta ctgctcgccg cgcacgcgat ctgcgaccac
     1921 ttgaccgtag aggtcgacct cgatcgcgct gttgatcgac acgacctcgt gattgcgcgc
     1981 gatcgtatgc ggcgcgttca ccacctcgac cgggaggaaa cggacgtcgg cgttcccgtc
     2041 gagccacgca tacagctcgg cggtaccgag cgcgaacgtg gtcaccgaga acccgttgaa
     2101 cacacccttg cgccggttcg tgactttgcc ggccttgtgc agctccatga gcccggtggt
     2161 gaacatctcg gaatgcacgc cgtagtcacc gccgccgtgc tcggcgagca acccgaccac
     2221 ggtcgacggc accgcaccga tcccggtttg cagtgtgcaa ccgtcgacgg cgaacgatgc
     2281 cgcgtgctgc gcaatggcgc gttcgacgtc ggtgggcggc gcgtcctcca tggcgtaggg
     2341 cggccgatcg ccgtgcacga gcacgtcgac ctcgtcgaca tgcagcgcgt gcgggtgctc
     2401 gggcggaagg ccgagcgtgc gcggcaatgc cgcgttcgct tcgacgatca gcaaacgctc
     2461 gtcgtcctgg cccgcgcgct tcagttcgcg gaccgttgcg ccggcgtgca gcgagaggct
     2521 cataaaaccg tgctcgtcgg gcggcgtcgc ggtcgtcgcc atgacccgcg gtgcgaagtc
     2581 gtcggcaatc gtcacgaaac gacgaaagtc cgcggggatg aactcgatgt tcgcgcccgt
     2641 gtcgcgtagg agtcgctctg ccggcccaaa gaaaccggtg agataacgga caccgggttt
     2701 cgagaacagc gcgtagaggt cgagcagcag cgcgccgaag atctcgaagc gctcccagtc
     2761 gtcgcgcgcg ccgagcgcgt gcagcaggct caccggttgc cccgggccga gcgggatgcc
     2821 gaggcggtcg accggccgga agcgcgccgc ggcttcttcc gctgtgcact cctgcatacg
     2881 cgtcaccggc cctgcgcgcg ccctcggccc aacgcttctt gcgcgaccga cgcgacgtgc
     2941 acgtcgttcg cgaacacacg cgcgaacgcg aggccgcgcg catcggcgac accggcgccc
     3001 cgcagctcga acagcagcca ctcgtccgcg cgcaccggcc ggtggaacca caacgcatgg
     3061 tcgaggctgg ccgtgaacag acggttccag tcgaatgcgt ccgggtgcgg cgt
//
LOCUS       DASUGA010000585         3299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_546163, whole genome shotgun sequence.
ACCESSION   DASUGA010000585 DASUGA010000000
VERSION     DASUGA010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546163"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VFX21_11515"
     CDS             complement(<1..507)
                     /locus_tag="VFX21_11515"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HEX5096638.1"
                     /translation="MTNGVPAPLLCIDAFTSEPFRGNPAAVCLLDEPLDDARMQAIAA
                     ELNLSETAFVTPGDDEYGLRWFTPTVEVVLCGHATLASAHALYETGRAAPGQPLSFST
                     RWRGILRATSSDDGIRLDFPAAPSTLVDPPAGLADALGAAPVAVGVNDLHHVVELSDA
                     ATVAALQPD"
     gene            complement(504..2279)
                     /locus_tag="VFX21_11520"
     CDS             complement(504..2279)
                     /locus_tag="VFX21_11520"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006181112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX5096639.1"
                     /translation="MLSKILIANRGEIAIRVMRTCRELGITTVAVYSELDRDAEHVRY
                     ADEAYALGGKTAAESYLNTEAILDAIKTSGADGVHPGYGFFSENADFARALIDAGVTW
                     IGPPPEAIEVMGDKISSRLAAQRAEVTSVPGTTTPITDVGEIHAFGEEHGYPIAIKAA
                     YGGGGRGMKVVADAQSAQSAFESAAREAQAYFGRPECYMERYLTRPRHVELQVFCDTH
                     GNGVYIGDRDCSAQRRHQKLIEEAPAPNIPDATRKAMGEAAVQVALACGYVNAGTVEM
                     LYQDGEFYFLEMNTRLQVEHCVTEEVTGLDLVAEQINVANGKKLSLTQKSVKIRGHAI
                     ECRINAEDPLKNFLPSPGTITKLRLPAGPGVRWDGGYFAGDTISQYYDNLVGKLVVWA
                     PDRDRAIERMVRALEEFEIEGVRTTIPADIALLQTDDFKNVNHSTKWVEDEVDQSSFA
                     QYAPAAAVAVAPPEDGDASALIERTVPVEVDGRRYSVKVWMPDAPVAAVAGARSGARP
                     RPKAAASGAGGTGDGTVTAPMQGTIVKVLVEVGATVEAGQAILVLEAMKMENHINAEA
                     GGTVAEVRVSAGDTVGTGDVLVVIE"
     gene            2325..3089
                     /locus_tag="VFX21_11525"
     CDS             2325..3089
                     /locus_tag="VFX21_11525"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX5096640.1"
                     /translation="MAQGRFTDVRRFDTIDSTNRYLLELAAAGAPDGVVAVADTQTAG
                     RGRLGRTWVAPPGASLLVSVLLRQNVAVTRAHLLTVAAGLAAIDAVRSLAAIDAQLKW
                     PNDVVVDDRKLAGILAEANGDAVVLGMGLNVHWTDFPPEIAQAATACNLESDIPVDRD
                     ALLDAWLATFAARLQDLDRVARDAVHASATLGRRVRVELPAEHFEAHAIALTPEGHLL
                     VRRADGAEVSVAAGDVVHLRIEGAGDEVAERGREAR"
     gene            complement(3033..>3299)
                     /locus_tag="VFX21_11530"
     CDS             complement(3033..>3299)
                     /locus_tag="VFX21_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX5096641.1"
                     /translation="TLYTPPAAAGCLLGVTRGLLLELCNDCTEADVPIDALARADEAF
                     LSSTTREVQPIAHVDGAALPAAPGPVSARLAAAFRDLVARTLDP"
BASE COUNT          439 a         1154 c         1187 g          519 t
ORIGIN      
        1 gtcgggttgg agtgcggcga cggttgccgc gtcgctcagc tcgacgacgt ggtgcaggtc
       61 gttcacgccc accgccaccg gcgcggcacc gagcgcatcg gcgaggccgg cgggcgggtc
      121 gacgagggtg ctcggcgcgg cggggaagtc gagccggatc ccgtcgtcgg acgaggtcgc
      181 ccgcaggatc ccgcgccacc gcgtgctgaa gctcaggggc tggccaggag cggcgcgccc
      241 cgtctcgtag agcgcatgcg cgctcgccag cgtggcgtgc ccgcagagca cgacctcgac
      301 ggtcggcgtg aaccaacgca ggccgtactc gtcgtcgccg ggggtgacga aggcggtctc
      361 ggagaggttc agctcggccg cgatggcctg catgcgagcg tcgtcgagcg gctcatcgag
      421 caggcagacg gcggccgggt tcccccggaa gggctcactg gtgaacgcgt cgatgcagag
      481 cagcggcgcg ggcacgccgt tggtcactcg atgacgacga gaacgtcgcc ggtgccgacc
      541 gtgtcgccgg cgctcacgcg cacctcggcg accgtgcccc cggcctcggc gttgatgtgg
      601 ttctccatct tcatcgcctc gagcacgagg atcgcctgcc cggcttccac cgtcgcaccc
      661 acctcgacga gcaccttcac gatcgttccc tgcatcggcg cggtcacggt gccgtcaccg
      721 gtaccgcccg cgccgctcgc ggctgctttc gggcgcggcc gcgccccgct gcgcgcgccg
      781 gcaactgccg cgaccggtgc gtcgggcatc cacaccttca cgctgtaccg gcgaccgtcg
      841 acctcgaccg gcaccgtgcg ttcgatgagc gccgacgcgt caccgtcttc gggcggcgcc
      901 acggcgaccg cggcggcggg cgcgtactgc gcgaacgacg actggtcgac ttcgtcctcg
      961 acccacttgg tggagtggtt gacgttcttg aagtcgtcgg tctgcaacag cgcgatgtcg
     1021 gcggggatgg tcgtgcgcac gccttcgatc tcgaactcct cgagcgcgcg caccatgcgc
     1081 tcgatcgcgc ggtcgcgatc gggtgcccac acgacgagct tgccgaccag gttgtcgtag
     1141 tactgcgaga tcgtgtcgcc cgcgaagtaa ccgccgtccc agcgaacgcc cgggccggcc
     1201 gggagccgca gcttcgtgat cgtgcccggc gatgggagga agttcttcag cgggtcctcg
     1261 gcgttgatgc ggcactcgat cgcgtggccg cggatcttca ccgacttctg cgtgagcgag
     1321 agcttcttgc cgttcgcgac gttgatctgc tctgcgacga ggtcgagtcc cgtcacctcc
     1381 tcggtgacgc agtgctcgac ctggagacgc gtgttcatct cgaggaaata gaactcgcca
     1441 tcctggtaca gcatctcgac ggtgcccgcg ttcacatagc cgcacgcgag cgcgacctgc
     1501 accgcggctt cgcccatggc cttgcgcgtc gcgtcgggaa tgttcggcgc gggtgcttcc
     1561 tcgatgagct tctggtgccg ccgctgcgcg gagcagtcgc ggtcaccgat gtacacgccg
     1621 ttgccgtgcg tgtcgcagaa cacctggagc tcgacgtgac ggggccgcgt caggtagcgc
     1681 tccatgtagc actcgggccg gccgaagtac gcctgcgctt cgcgcgccgc ggactcgaac
     1741 gcgctctggg cgctctgtgc gtccgcgacg accttcatgc cgcgcccgcc gccgccgtag
     1801 gcagccttga tcgcgatcgg gtagccgtgc tcctcgccga aggcgtggat ctcgccgacg
     1861 tcggtgatgg gcgtcgtcgt gccgggcacc gacgtgacct cggcgcgctg cgccgcgagg
     1921 cgcgacgaga tcttgtcgcc catgacctcg atcgcctcgg gcggcggtcc gatccaggtg
     1981 acgcccgcgt cgatcagcgc gcgagcgaag tcggcgttct cggagaagaa gccgtagccg
     2041 gggtgcacac cgtcggcgcc cgacgtcttg atcgcgtcga ggatcgcctc ggtgttgagg
     2101 tagctctcgg ccgcggtctt gccgcccagc gcgtacgcct cgtcggcgta gcggacgtgc
     2161 tcggcgtcgc ggtcgagctc ggaatacacg gcgaccgtgg tgatgcccag ttcgcgacac
     2221 gtccgcatga cgcggatcgc gatctcgcct cggttcgcga tgaggatctt cgacagcaat
     2281 gacgcccctc ggccggccgg caacggcggc ctactctacg ggtcatcgcc cagggacgct
     2341 ttaccgacgt tcgccgcttc gacacgatcg attccaccaa ccgctatctg ctcgagctcg
     2401 cggccgcggg cgcgcccgac ggcgtcgtcg ccgtcgccga cacgcagacc gcgggccggg
     2461 gccggctcgg gcgcacgtgg gtggcgccac ccggcgcgtc gctcctggtg tcggtgctgc
     2521 tccgtcagaa cgtcgcggtc acgcgcgcac atctgctgac cgtcgcggcg gggctcgccg
     2581 cgatcgatgc cgtgcggtcg ctcgcggcga tcgacgcgca gctgaagtgg ccgaacgacg
     2641 tggtcgtcga cgaccgcaag ctggccggga tcctcgcgga ggccaacggc gacgcggtcg
     2701 tgctcggcat gggtttgaac gtgcactgga ccgacttccc gcccgagatc gcgcaggccg
     2761 cgactgcgtg caacctcgag tccgacattc cggtcgaccg ggacgcactg ctcgacgcgt
     2821 ggttggcgac gttcgcggcg cgactccagg atctcgaccg tgtcgcccgc gacgcggtgc
     2881 acgcgtcggc gacgctgggc cggcgcgtgc gcgtcgaact gcccgccgag cacttcgaag
     2941 cgcacgcgat cgcgctgact cccgaagggc acttgctcgt gcggcgggcc gacggtgccg
     3001 aggtgtcggt cgccgcgggc gacgtcgtgc acctacggat cgagggtgcg ggcgacgagg
     3061 tcgcggaacg cggccgcgag gcgcgctgag accggaccgg gcgcggccgg gagcgcggcg
     3121 ccgtcgacgt gcgcgatcgg ctgcacctcg cgtgtcgtcg acgacaggaa cgcttcgtcg
     3181 gcccgcgcga gcgcgtcgat cgggacgtcg gcctcggtgc agtcgttgca cagttcgagg
     3241 agcaaaccgc gcgtgacgcc cagcaggcag ccggcggccg ccggcggcgt gtagagcgt
//
LOCUS       DASUGA010000586         3755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_108324, whole genome shotgun sequence.
ACCESSION   DASUGA010000586 DASUGA010000000
VERSION     DASUGA010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108324"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VFX21_11535"
     CDS             complement(<1..318)
                     /locus_tag="VFX21_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096642.1"
                     /translation="MADDEARENDHEGEAPVAAPAPGDEVAAALLARFDGSVNASSHG
                     QPVVYVDRAVFADVARYLRDEEQFSMLCDITAVDHLLDGTRYAPPGVERLERFEAVAN
                     YLSH"
     gene            complement(341..886)
                     /locus_tag="VFX21_11540"
     CDS             complement(341..886)
                     /locus_tag="VFX21_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX5096643.1"
                     /translation="MDIGSVPHNFLTGRLEDVVRWGRRNSVFPATFGLACCALEMMAT
                     GAAHYDLARFGMEVFRASPRQADLMIVAGRVSQKMAPVLRQVYDQMVEPKWVISMGVC
                     ASSGGMFNNYAIVQGVDQIVPVDVYVPGCPPGPETLMHGILTLHEQIRTGELMKRRAV
                     SKSGGAEIDMAREPQPVTLSS"
     gene            complement(886..1353)
                     /gene="ndhC"
                     /locus_tag="VFX21_11545"
     CDS             complement(886..1353)
                     /gene="ndhC"
                     /locus_tag="VFX21_11545"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX5096644.1"
                     /translation="MADYLPLLIMAGVVIAFVFLSFVASQLLAPKRPNSAKQAPYECG
                     IVPEDEPAERFPVKFYLVAMSFIVLDVEIVFLYPFATVFRDLGTFGFVAMSVFVLALL
                     IPFAYLLSVGALEWSPMKDIVKRTAGPLLRTPNAMFDPAARAQPAETETEEAA"
     gene            complement(1439..2686)
                     /locus_tag="VFX21_11550"
     CDS             complement(1439..2686)
                     /locus_tag="VFX21_11550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HEX5096645.1"
                     /translation="MSSTFDVLVVGGGPSGAATAYWLAERGHRVLVVEKKRFPREKTC
                     GDGLTPRAVRQLHDMGLADGLAGYQRYDGLRSIAHGITLELAWPEHPDFPQYGYVVRR
                     RDLDEMVAGQAVKAGATLWPAAEALAPVVENGLITGAQVQHKDTGVVEAVGATYVVVA
                     DGANSRFGRALGTARDRTYPLGMAIRGYFTSPRHDDPWIESHLDIHDKDGNNLPGYGW
                     VFPVGDGTVNVGVGLLSTFAGWTSVNTTRLMEAFCATAPDYWDIRPETATCAPTGGRL
                     PTGCSVLPRVGPTFVVVGDAAGSVNPFNGEGISVAYETGRLAADAVDLALRTNDGLAL
                     RTYDERLTEVYGLYFKVARAFVRAIGNPAVMRELTRVGMHSRTLMEWVLRIMANLLRP
                     DELGPAEAAYKAVAALVKVSPEP"
     gene            2885..3481
                     /locus_tag="VFX21_11555"
     CDS             2885..3481
                     /locus_tag="VFX21_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096646.1"
                     /translation="MLGLEIPNFLPRSVPWPQWDDGLSWGHSHWVQHGGRLIWATVAF
                     VIGLVIIAVLIRRPKSPEPATWAASIGGAFFVWLMMVLAYAIIPHEWIIFANSYLQWG
                     KDKFFLQQDQIATNLPPFSVQRYVLSDMIAAGFYVVFGVLNVYLFSKWQKRPVAEPVA
                     VEDTDETAPADPLTGPFSRLRRRSSRTSAYGRPVTTGD"
BASE COUNT          641 a         1263 c         1260 g          591 t
ORIGIN      
        1 gtgcgacaag taattggcga cggcttcgaa gcgctccaac cgctcaacac cgggcggcgc
       61 gtagcgcgtg ccgtcgagca gatggtcgac cgcggtgatg tcgcacaaca tcgagaactg
      121 ctcttcgtcg cgcagatacc gcgcgacgtc ggcgaacacc gcgcggtcga cgtagacgac
      181 gggctggccg tgcgaggagg cgttcaccga tccgtcgaag cgcgcgagca acgcggccgc
      241 gacctcgtcg cccggcgcgg gcgcggcaac gggcgcttcg ccttcgtgat cgttttcacg
      301 agcctcgtcg tcggccattc ggtctgcgtg cctcttgcgg tcaggaactc agcgttacgg
      361 gctggggttc gcgcgccatg tcgatctcgg ctccccccga cttggacacc gcgcgccgtt
      421 tcatcagttc gccggtgcga atctgctcgt gcaacgtgag gatgccgtgc atcagcgtct
      481 cggggccggg aggacagccc gggacgtaca cgtcgacggg gacgatctgg tcgacaccct
      541 gcacgatcgc gtagttgttg aacatgccgc cgctgctcgc gcacacgccc atggagatga
      601 cccacttggg ctcgaccatc tggtcgtaga cctggcgcag cacgggagcc atcttctgcg
      661 agacgcggcc cgcgacgatc atcaggtcgg cctggcgcgg gctggcgcgg aacacctcca
      721 tgccgaagcg cgcgaggtcg tagtgcgcgg cgccggtggc catcatctcg agcgcgcaac
      781 acgcgagacc gaacgtcgcg gggaacaccg agttgcgtcg accccaccgc acgacgtctt
      841 cgaggcgacc ggtgaggaag ttgtgcggta ccgacccgat gtccatcagg ccgcctcttc
      901 ggtctcggtc tccgccggct gtgcgcgcgc ggcgggatcg aacatcgcgt tcggtgtgcg
      961 caacagcgga cccgcggtgc gcttgacgat gtccttcatc gggctccact cgagcgcgcc
     1021 gacggacagc aggtacgcga acggaatgag cagcgccaac acgaagaccg acatcgcgac
     1081 gaacccgaac gtacccagat cgcggaacac ggtcgcgaag ggatagagaa agacgatctc
     1141 cacgtcgagg acgatgaagc tcatcgcgac cagatagaac ttcaccggga agcgctcggc
     1201 cggttcgtct tcggggacga tgccgcactc gtacggcgcc tgcttggccg agttgggccg
     1261 cttcggcgcc aggagctgcg acgccacgaa cgacaagaag acgaacgcga tgaccacacc
     1321 ggccatgatc aggagtggca gatagtcagc caacgcgtcg tcgtcccctc gggcggaact
     1381 tgcgcgcgga cactaccgcg cgacccccgc gctgaaccgt ttcaagcccg ttcgtggact
     1441 acggctccgg ggagaccttc accagcgcgg cgacagcttt gtaggcagcc tccgcggggc
     1501 cgagctcgtc ggggcgcaag aggttggcca tgatccgcaa cacccactcc atgagggtgc
     1561 ggctgtgcat cccgacgcgt gtcagctcgc gcatgaccgc ggggttgccg atggcgcgca
     1621 caaatgcacg agcgaccttg aagtagaggc cgtacacctc ggtcaggcgc tcgtcgtagg
     1681 tgcgcagcgc gaggccgtcg ttggtacgga gggcgaggtc gacggcgtcg gcggcgaggc
     1741 ggccggtctc gtaggcgacc gagatcccct caccgttgaa cgggttgacc gagccggcgg
     1801 cgtcgccgac caccacgaac gtggggccga cccggggcag caccgaacat ccggtgggca
     1861 gccgccctcc cgtgggtgca caagtggccg tttcggggcg aatatcccaa tagtcgggcg
     1921 cggtggcgca gaacgcctcc atcagccggg tcgtgttcac ggaggtccag cccgcgaacg
     1981 tcgacagcag gccgacgccg acgttgaccg tgccgtcgcc gaccgggaag acccagccgt
     2041 agcccgggag gttgttgccg tccttgtcgt ggatgtcgag gtggctctcg atccagggat
     2101 cgtcgtgccg ggggctcgtg aagtagcccc ggatcgccat ccccagggga taggtgcggt
     2161 cgcgggcggt gcccagcgcc cggccgaagc gcgagttggc gccgtcggcg accacgacgt
     2221 aggtggcccc gaccgcctcg accacgccgg tgtccttgtg ctggacctgg gcccccgtga
     2281 tcaggccgtt ctcgaccacc ggggccagcg cctcggcggc cggccacaag gtggcgccgg
     2341 ccttgaccgc ctggccggcg accatctcgt cgaggtcgcg ccgccgcacc acgtacccgt
     2401 actgggggaa gtcggggtgc tcgggccagg ccagctcgag cgtgatgccg tgcgcgatcg
     2461 accgcagacc gtcgtagcgc tggtaccccg cgagcccgtc ggcgaggccc atgtcatgca
     2521 gttggcggac ggcgcgcggc gtcagcccgt ccccgcaggt cttctcgcgc gggaagcgct
     2581 tcttctccac gacgagcact cgatggccgc gctccgcgag ccagtacgcg gtcgctgcgc
     2641 cactcggtcc accaccgacg acgagcacgt cgaacgtcga gctcacgaag cacatattca
     2701 catgcgcggg cggctcggaa cgcatcgtcg acggcgcaga aaaacgtccc tgcatgatgt
     2761 gatgtgggag cattcaaggt ggtagcaagg gctcgatcac gagcctgctt ccgggctcat
     2821 cggaaccgcg ccgcatcggc acaagatcga gagagaattc ggggtgtccc acatcacttc
     2881 gagcgtgctt ggtctcgaaa tcccgaactt cctaccgcgc agtgtgccgt ggccgcagtg
     2941 ggacgatggc ctctcgtggg gtcactcgca ctgggtgcag catggcgggc gactgatctg
     3001 ggcgacggtc gcattcgtga tcggcctcgt gatcatcgcg gtgttgatcc gtcgcccgaa
     3061 gagtcccgaa ccggcgacgt gggccgcgag catcggcggc gcgttcttcg tgtggctgat
     3121 gatggtgctc gcctacgcga tcatcccgca cgagtggatc atcttcgcca actcgtacct
     3181 gcagtggggc aaggacaagt tcttcctgca acaggatcag atcgcgacga acctgccgcc
     3241 gttctcggtc cagcggtacg tgctctccga catgatcgcc gcgggcttct acgtcgtgtt
     3301 cggcgtgttg aacgtgtacc tgttctcgaa gtggcagaag cgcccggtcg cggaacccgt
     3361 cgccgtcgaa gacaccgacg agaccgcgcc cgccgatccg ctgaccggtc cgttctcgcg
     3421 cctgcgccgc cgctcgtccc gtacttccgc gtacggccgt cccgtgacga caggcgattg
     3481 aacatgatgc aacgactcga ctccgtcaac gcggcgagcg tcagcgagct cgccagcccg
     3541 gcgaccggcc gccaagccgg catcacacgc gcgcgcgagg gagtgggcga atagacatgg
     3601 ctcgcaccga cgccaatccg ccgatgccgg agttctccac cgactacgtg ctgcaagagg
     3661 tcgaccccaa gtggctcacc accgcggtca aacccaagca gttcctccac atcgaccaag
     3721 ccgaatgcat cctctgcgaa ggctgcgtcg acatc
//
LOCUS       DASUGA010000587         3621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2002147, whole genome shotgun sequence.
ACCESSION   DASUGA010000587 DASUGA010000000
VERSION     DASUGA010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2002147"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1464
                     /locus_tag="VFX21_11560"
     CDS             100..1464
                     /locus_tag="VFX21_11560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEX5096647.1"
                     /translation="MKKKREDLKRLLGELGAFQQKMGSDAPPTAAEAEAMDAKAHEAE
                     GLQAEIKAHDDRQKRYGKLQIDGKTIVDAALPNDEAKLDDDDFEPVQRSSRRARTKGG
                     KPIAGYMRLGDYVVGSKGFADYQRNGLPKNGAMLADVPTLLKDRQGQVLVALTGPEIK
                     SMMELKAVPTIGDTVIEPTLVPDFVRVLEQDRLRLRDILDVSSTTSDAVKYTRLVSYT
                     RGAATVAPSALKPEAAMELDTVTEAVRTIAVHIPVEEQQLADYPALSGIINNELLYDL
                     DKHLEELIAWGDGTGENFLGIMNDPDVLAARSEAGDTLIDIARRGITDVRRAGYEPNG
                     ILADPLDWEDIVLLKGTDEKYIWVVVTEGETQRLWGVPVIETVAMEDFQGVATEERGL
                     LVGDFRRGATLWDRQRSSISVGFINDQFIRNQRTILAELRAAFGVKRPGAFRRYQTQA
                     PSAS"
     gene            1553..2146
                     /locus_tag="VFX21_11565"
     CDS             1553..2146
                     /locus_tag="VFX21_11565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5096648.1"
                     /translation="MAVTRAVYRDRTELFRRLFPRDGVVVEIGSQRGVSARAIVHACQ
                     PRLLVLVDPWRKIGGAYENDPADRPTRTREEEMRVCAARVGCMSNVAMLRCLSHEANA
                     LFADGSCDAVYLDGDHSYEGVAHDIEMWWPKVKSGGVLAGHDYTIALPWVGVMRAVDE
                     WVVREGLDLQVTESSQPRGGTEDKDGALFPSWAVVKP"
     gene            2143..2967
                     /locus_tag="VFX21_11570"
     CDS             2143..2967
                     /locus_tag="VFX21_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEX5096649.1"
                     /translation="MNFVTYSDLARDTRALARKLPRDTYLVLGVPRSGMIPASIVAQE
                     LGCHLGDVGTYARTRTFMAPGKRMTWTPDPAGPIVVVDDSLFRGSTMADATAPLAGMN
                     ILRAAVYVAPGKEDAVDVYARAVAAPRLFEWNWLGSSLLERSLCDMDGILCEDSAVYD
                     DDSEAYAESLRLAKPLFVPRRKIGAIVTARLERWRTLTQVWLDVHRISVGRLVMAPFP
                     TANARRRHGNAKWKASVYETAKDSLVFVESSVRQAREIAELTGRDVLCPGVGRLFR"
     gene            2964..>3621
                     /locus_tag="VFX21_11575"
     CDS             2964..>3621
                     /locus_tag="VFX21_11575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX5096650.1"
                     /translation="MNETIAIVTSCRNYGRYLSDWADSIIKLTRRSDVVCIVDDASTD
                     QTPDFVTKAASKLLAAGIPTRTLRIEERNFGKARNAAVRLAGDVTWVQHLDCDDMAMP
                     HMLDDWAALAPTADVVAIGYERVGDLKSGPSNRTKVYKSSRGKSTLESQCPASGVSPF
                     RREFWERRPYVEDMNGGWDTALWIGFGHLNARFVATRRPGFMYRQHGDSIFNRRRKHE
                     R"
BASE COUNT          635 a         1148 c         1275 g          563 t
ORIGIN      
        1 ctcgcctcgc cactcgcatc gacgcgatcc ggcacgcgag cacggaactg actctgtaac
       61 accaccacca ccttcaacgc gaggatccga atggatgcga tgaagaagaa gcgcgaggac
      121 ctcaagcgac tgctcgggga actcggcgcg ttccagcaga agatgggcag cgacgcgccg
      181 cccacggcgg ccgaggccga ggcgatggac gccaaggcgc acgaggccga gggcctccag
      241 gccgagatca aggcgcacga cgatcggcag aagcggtacg gcaagctcca gatcgacggc
      301 aagaccatcg tcgacgcggc gctgccgaac gacgaggcga agctggacga cgacgacttc
      361 gagcccgtgc agcggtccag ccgccgcgcg cgcacgaagg gcggcaagcc catcgccggc
      421 tacatgcggc tgggcgacta cgtcgtcggg tccaaggggt tcgcggacta ccagcgcaac
      481 gggctcccga agaacggcgc gatgctggcc gacgtgccga cgctgctgaa ggaccggcag
      541 ggtcaggtgc tggtcgcact gaccggaccc gagatcaagt ccatgatgga actcaaggcc
      601 gtgccgacga tcggcgacac ggtcatcgag cccacgctgg tgcccgactt cgttcgggtg
      661 ctggagcagg atcggcttcg gctgcgcgac atcctcgacg tgtcgtccac cacgtcggat
      721 gcggtgaagt acacgcgcct cgtgtcgtac acgcgcggcg cggccaccgt cgcaccttcg
      781 gccctcaagc cggaagccgc gatggagttg gacacggtga ccgaggccgt gcgcacgatc
      841 gccgtgcaca tcccggtcga ggagcagcag ttggccgact accccgcgct gtcgggcatc
      901 atcaacaacg agcttctcta cgacctcgac aagcacctcg aggaactgat cgcgtggggc
      961 gacgggacgg gcgagaactt cctcggcatc atgaacgacc ccgacgtgct ggccgcgcgt
     1021 tcggaggcgg gtgacacgct catcgacatc gcgcgtcgcg gcatcacgga cgtgcgtcgt
     1081 gccgggtacg agccgaacgg catcctcgcg gacccgctcg actgggagga catcgttctc
     1141 ctgaagggca ccgacgagaa gtacatctgg gtcgtcgtca ccgagggcga gactcagcgg
     1201 ctgtggggcg tgcccgtcat cgagacggtc gcgatggagg acttccaggg cgtggcgacc
     1261 gaggagcgcg ggctgctcgt cggagacttc cgccgtggcg cgacgctgtg ggaccggcag
     1321 cggtcgtcca tcagcgtcgg gttcatcaac gaccagttca tccgcaacca gcggacgatc
     1381 ctggccgagc ttcgcgctgc gttcggcgtg aagcgccccg gcgcgttccg caggtaccag
     1441 acgcaggctc cgtcggcgag ctaaccgacg acgcaggagg aattgcgaca agccggcggg
     1501 tggcacggtg ccgcccgccg gcactcctca aaacttcgag cacgtgtgag taatggcggt
     1561 tacaagggca gtgtaccgtg accgcaccga actctttcgg cggctgttcc cgcgcgatgg
     1621 cgtagttgtc gagatcgggt cgcaacgggg tgtgtcggca agggcgatcg tgcacgcgtg
     1681 ccaaccgcgc ttgttggtgc tggtggaccc gtggcgcaag atcggcggtg cgtacgagaa
     1741 cgacccggcg gatcgtccca cgcgcacgcg cgaggaggag atgcgtgtgt gcgcggcgcg
     1801 cgtcgggtgc atgtcgaacg tcgcgatgct gcggtgtctg tcgcacgaag ctaatgcgct
     1861 tttcgcggac ggttcgtgtg acgccgtgta tctcgacggc gaccactcgt acgagggcgt
     1921 ggcgcacgac atcgagatgt ggtggcccaa ggtgaagtca ggcggcgtgt tggccgggca
     1981 cgattacacg atcgcgctgc cctgggttgg cgtgatgcgc gcggtcgacg agtgggtcgt
     2041 gcgtgaagga ctcgacttgc aggtcaccga atcctcgcaa ccgcgcggcg gcaccgagga
     2101 caaagacggc gcgctgttcc cgtcgtgggc ggtagtgaag ccgtgaactt cgtcacgtac
     2161 tccgacctcg cgcgcgacac gcgcgcgctc gcgcgcaagc tgccgcgcga cacgtacctc
     2221 gttctgggcg tcccgcgctc ggggatgatt cccgcatcca tcgtggcgca ggagcttggc
     2281 tgccacctgg gcgacgtggg cacgtacgcg cgcacgcgga cgttcatggc tcccggcaag
     2341 cgcatgacgt ggacccccga cccggccggg cccatcgtcg tcgtcgacga ctcgctgttc
     2401 cgtgggtcga cgatggccga cgcgacggcc ccgctggcgg gcatgaacat cttgcgtgcg
     2461 gcggtctacg tggctccggg caaggaagac gccgtcgacg tgtacgcccg cgccgtggcc
     2521 gcaccgcgct tgttcgaatg gaactggctc gggtcgtcgc tgctcgaacg ttcgttgtgc
     2581 gacatggacg gaatcctgtg tgaagactcg gccgtgtacg acgacgacag cgaggcgtac
     2641 gccgagtcgt tgcgacttgc caagccgttg ttcgttccac gtcggaagat tggtgcgatc
     2701 gtgactgctc gcctggagcg gtggcgcacg ctcacgcaag tgtggttgga cgtgcaccgc
     2761 atctccgtcg ggcggctggt catggctccg ttcccgaccg ccaacgcgcg ccgccgtcac
     2821 ggcaacgcga agtggaaggc gtccgtgtac gagacggcca aggacagctt ggtgttcgtc
     2881 gagagcagcg tgaggcaggc gcgtgagatc gcggaactga ccgggcgcga tgtgttgtgc
     2941 cctggcgtgg ggaggttgtt ccggtgaacg aaaccatcgc catcgtcaca agctgccgga
     3001 actacgggcg ctacctgagc gattgggccg attcgatcat caagctcacg cgcaggtccg
     3061 acgtcgtgtg catcgtcgac gacgcgtcga ctgaccagac gccggacttc gtgacgaagg
     3121 cggcgagcaa gttgctggcg gcgggcatcc cgacgcgcac gttgcgcatc gaggaacgca
     3181 acttcggcaa ggcgcgcaac gccgccgtac ggctggccgg tgacgtgacg tgggtccagc
     3241 acctcgactg cgatgacatg gcgatgccgc acatgttgga cgactgggcc gcgctcgcgc
     3301 ccaccgccga cgtcgtcgcc atcgggtacg agcgcgtcgg cgatctcaag agcgggccgt
     3361 cgaaccgcac caaagtttac aagagcagcc gaggcaagag cacgctcgaa tcgcagtgtc
     3421 ccgcgtcggg tgtgtcaccg ttccgccgcg agttttggga gcgccgcccg tacgtcgaag
     3481 acatgaacgg agggtgggac acggcgttat ggatcgggtt cggccacttg aacgcgcgct
     3541 tcgtcgcgac gcgacggccc gggttcatgt accgccaaca cggggactcg atcttcaacc
     3601 gtcggcgcaa gcacgaacgc g
//
LOCUS       DASUGA010000588         3195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1029913, whole genome shotgun sequence.
ACCESSION   DASUGA010000588 DASUGA010000000
VERSION     DASUGA010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029913"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VFX21_11580"
     CDS             <1..825
                     /locus_tag="VFX21_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096651.1"
                     /translation="RSMWNFWRGDHLTVRNVIVVGANPNAGAEGNAYVAALEGQAGFV
                     IGGVQNMLMERVQVYDVYGDFVHVGAATQDLLVRYSTFDRNGRQGWNIAGTNITFDGN
                     IIRNTARATIDMEPPSTTAGANHIVIMNNKGGKGRLYFFASVGVGAPINDVQILNNTL
                     TKALTMTVRAPAAQPRTNYTVAGNVSTQLYGGNGGAFSFVNVYGIDIHDNVQPTAKYR
                     QPHGVALMDSRDASITNNTFLNADGALLDRGGNQNVTQSGNWGGTPLAPFPPSSIP"
     gene            complement(836..1294)
                     /locus_tag="VFX21_11585"
     CDS             complement(836..1294)
                     /locus_tag="VFX21_11585"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX5096652.1"
                     /translation="MSEDTTPDTTLRIERLIDAPPEVVFDAWTKPEAMEVWYADGPNG
                     HARVVEQDLRVGGRFRIQWGPDKTAQYEEHGEYLVLDRPRQLVLTETLVAAEGGWQET
                     RVTVTFENVDGKTRLTLVHEKMPSKEARDGAAGGWPGFLDRLERYAIANR"
     gene            complement(1291..1620)
                     /locus_tag="VFX21_11590"
     CDS             complement(1291..1620)
                     /locus_tag="VFX21_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5096653.1"
                     /translation="MSSLKVPVDQTFAALADPTRVEIVVRLHRGSATVSELAEPYDMS
                     LRAVLKHVQVLEAAGLVRTVKDGRVRRCELERRRLDEAERWMDDLRRRWERRLDRIEA
                     YIEREKE"
     gene            complement(1669..2481)
                     /locus_tag="VFX21_11595"
     CDS             complement(1669..2481)
                     /locus_tag="VFX21_11595"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5096654.1"
                     /translation="MKVDTGDVTLDVEVHGEGRPLMLVHGFPDTKRLWSKMVPHLVGA
                     GFQVITYDQRGYGASDKPADVAAYSIPFLANDVTEVLDELGIEKAHLAGHDWGAAVVW
                     AAATFAPDRVDHLIAMSVGHPSAFARADMAQRQKSWYMLLFQWEGIAEEWLTKDDAVN
                     FYDWCHHPDFRAVLADLERDKSLTPGLNYYRANVPPAALTGPPLELPPIQAPTMGIWS
                     SNDFALNEPQMFESAKYCANGFRYERIDGVDHWMQWEAPERVTPLFLDFLPR"
     gene            complement(2492..>3195)
                     /locus_tag="VFX21_11600"
     CDS             complement(2492..>3195)
                     /locus_tag="VFX21_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5096655.1"
                     /translation="LPADNGTWGIGVVTSAKDKELRALKDVDAWMRVVKSFPLQAHWL
                     DGEPLNDSIAVMAKIEDRHRSFVVDGQPVATGVFSVGDSWAATNPSVGRGMTIGAMHA
                     VALRETLNNAADDPVELAQQWHDATMASVEPWYRATLAFDAARLDEIHAGLEGREFQP
                     DAQYEITLAMMSAAGKAPDTFRSAMQIVGVLRTPEEVFAEPGVFERVVELGSNWRNEV
                     PVGPSREELLAAVAG"
BASE COUNT          544 a         1074 c         1005 g          572 t
ORIGIN      
        1 cgcagcatgt ggaacttttg gcgcggcgac cacttgacgg tgcggaatgt catcgtcgtc
       61 ggcgccaatc cgaacgcagg cgccgaaggg aacgcgtacg tggccgcgct cgagggccag
      121 gcagggttcg tcatcggcgg cgtccaaaac atgctcatgg agcgggtaca ggtctacgac
      181 gtgtacggcg actttgtgca cgtcggcgcg gcaacgcagg acctgctcgt gcggtactcg
      241 acgtttgatc gcaatggccg ccagggctgg aacatcgccg gcacgaacat cacgttcgac
      301 ggaaacatca tccgtaatac ggcgcgcgca accatcgaca tggaaccgcc ctcgacgacc
      361 gcgggcgcaa atcacatcgt gatcatgaac aacaagggcg gcaagggtcg cctctacttc
      421 ttcgcgagtg tgggtgtcgg cgcgccgatc aacgacgtgc agatcctcaa caacacactg
      481 accaaggcgc tgacgatgac ggtgcgcgcg ccggcggcgc aaccgcgcac gaactacacc
      541 gtggccggga acgtcagcac gcagctctac ggcggcaacg gcggcgcttt cagcttcgtg
      601 aacgtttacg gcatcgacat tcacgacaac gtgcagccga cggcgaagta ccgtcagccg
      661 cacggcgtcg cgctgatgga ctcgcgcgac gcgtcgatca cgaacaacac gttcttgaac
      721 gcggacggcg cattgctgga ccgaggcgga aaccagaacg taacccagtc ggggaactgg
      781 ggcggcacgc cgctcgcccc gttcccaccg tcgtcaattc cgtaggctcg attcgctagc
      841 ggttggcgat cgcgtaccgc tccaagcggt cgaggaaccc gggccagccg ccggccgcac
      901 catcgcgcgc ttccttcgac ggcatctttt cgtgcaccag cgtgagtcgc gtcttgccgt
      961 cgacgttctc gaacgtgacg gttacgcgcg tctcctgcca gccgccttcc gcggcaacca
     1021 gcgtttccgt cagcaccagc tgacggggcc ggtcgaggac gaggtattcg ccgtgctctt
     1081 cgtactgcgc agttttgtcc ggcccccatt ggatacggaa ccggccgccg acgcgcaggt
     1141 cttgctcgac aacgcgtgcg tgcccgttcg gcccgtcggc gtaccagacc tccatcgctt
     1201 cgggcttggt ccacgcgtcg aacaccactt cgggcggcgc atcgatgagt cgttcgatgc
     1261 gtaaggtcgt gtcgggagtc gtgtcttcgc tcattccttt tccctttcga tgtacgcctc
     1321 gatgcgatcg agccgccgtt cccaacgccg gcgcaggtca tccatccagc gttcagcctc
     1381 gtcgagccgg cggcgctcca gctcgcaacg acgtacgcgt ccgtctttca cggtgcgcac
     1441 caatcccgcg gcttcgagca cctgcacgtg cttgagcacc gcgcgcaacg acatgtcgta
     1501 gggctcggcc aactcgctga ccgtcgccga accgcggtgc aaccgcacca cgatctcaac
     1561 ccgggtcggg tcggcgagcg ccgcaaatgt ctgatcgaca ggaaccttta gtgaactgat
     1621 tggttcagta tcgcgaacgc gatcgtcgcc gtcaagcgcg tttgtcgttc agcgcggcag
     1681 gaagtcgagg aacaatggcg tgacgcgctc gggcgcttcc cactgcatcc agtggtcgac
     1741 gccgtcgatg cgctcgtaac ggaagccgtt cgcgcagtac ttcgcagact cgaacatctg
     1801 cggttcgttg agtgcgaagt cgttgctcga ccagatgccc atcgtcggag cctggatcgg
     1861 tggcagttcg agcggcgggc ccgtgagcgc ggccggcggg acgttggcgc ggtaatagtt
     1921 gagacccggc gtcaacgact tgtcgcgttc gagatcggcg aggaccgcgc ggaagtcagg
     1981 gtggtggcac cagtcgtaga agttcaccgc gtcgtccttc gtcaaccact cttccgcgat
     2041 cccttcccac tggaagagca gcatgtacca ggacttctga cgctgagcca tgtcggctcg
     2101 tgcgaacgcg ctgggatgtc cgaccgacat cgcgatgagg tggtcgactc gatcgggcgc
     2161 gaacgtcgcg gccgcccaca ccacggccgc tccccagtcg tgcccggcga ggtgtgcctt
     2221 ctcgatgccg agctcgtcga gcacctccgt cacatcgttg gccaggaacg gaatcgaata
     2281 cgccgcgacg tcggcgggct tgtcgctcgc gccgtagccg cgctggtcgt acgtgatgac
     2341 ttggaagccg gcaccgacga gatgcggcac catcttcgac cacagacgct ttgtgtcggg
     2401 gaacccgtga acgagcatga gcggccggcc ttcgccgtgc acctcgacgt cgagcgtcac
     2461 gtcgccggta tcgaccttca tcgttccccc actatcccgc caccgccgcg agcaattctt
     2521 cgcggctcgg tccgaccggc acttcgttgc gccagttgct gcccaactcg acaactcgtt
     2581 cgaagacgcc cggctcggcg aacacttcct cgggcgtccg cagcacaccg acgatctgca
     2641 tcgcgctccg gaacgtgtcg ggcgccttgc cggccgccga catcatcgcc aaggtgattt
     2701 cgtattgcgc gtcgggttgg aactcgcggc cttcgagtcc ggcgtggatt tcgtcgagac
     2761 gcgcggcgtc gaacgcgagg gtggcgcggt accacggctc caccgacgcc atcgtggcgt
     2821 cgtgccactg ctgcgcgagt tcgacgggat cgtcggcggc gttgttcagg gtttcgcgca
     2881 acgcgacggc atgcatcgcg ccgatcgtca tgccgcgccc cacggacgga ttcgtcgcgg
     2941 cccacgagtc gcccaccgag aacacgccgg tcgcaaccgg ttggccgtcg accacgaacg
     3001 aacggtggcg atcctcgatc ttcgccatca ccgcgatgga gtcgttgagc ggttcgccgt
     3061 cgagccagtg tgcctgcaac gggaagctct tcacgacgcg catccacgcg tcgacgtcct
     3121 tgagcgcgcg aagctccttg tccttcgcgc tcgtgaccac gccgatcccc cacgtgccgt
     3181 tgtcggccgg tagcg
//
LOCUS       DASUGA010000589         2527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1101337, whole genome shotgun sequence.
ACCESSION   DASUGA010000589 DASUGA010000000
VERSION     DASUGA010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101337"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..806
                     /locus_tag="VFX21_11605"
     CDS             42..806
                     /locus_tag="VFX21_11605"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX5096656.1"
                     /translation="MLLTVDAGNTQTVVGLFNEAELVDHWRIATVADRTSDELALMVQ
                     QFLGFHGFSFDDSITGVAISSGVPRVTAELRQMTERYFGFDALVLEPGVRTGMPILYD
                     NPKEVGADRIANAVGAYDLYGGPSIVVDFGTATTIEAVSERGEYMGGAIFPGVEISMD
                     ALFGRAAGLRRVELIEPKHVIGRNSSESIQSGMLYGFSAQVDGLVDRFVAELGPCEVI
                     ATGGLAEPIIEFSRTVQHYEPWLTLYGLRIIFERNR"
     gene            803..2311
                     /gene="lysS"
                     /locus_tag="VFX21_11610"
     CDS             803..2311
                     /gene="lysS"
                     /locus_tag="VFX21_11610"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX5096657.1"
                     /translation="MSPEAPSSEPNAEFQQRLAKVEEIRARGDDPYPVKFDRTHSLGE
                     VRRVWDDKVDVGASTDDVVRVAGRVLLLRDMGKLAFATVRDGTGDIQFFVSKGEVGDD
                     AFARFVAEVDRGDWVGATGTVMKTKKGELSVKISEFSLLSKALRPLPEKWHGLSDTDT
                     RYRQRYVDLIANDDARRVFDVRFTAVNAIRQFLLERGYVEVETPVLHPIAGGATARPF
                     STHHNALDSELFLRIAPELYLKRLIVGGYDRVFELARVFRNEGLSTRHNPEFTMLELY
                     EAFADYHDIARLTEEMIAAAARAATGGTIVEWDGEEVDLTPPFQRRTLLELIEEHAGV
                     KVHPSQPIEELRAVCDQLGVAWDKKDRPGKLVLEIYEKTTEANIKGPTFVMDYPRDVS
                     PLAREHRDDPTMTERFELIVRGRELGNAFSELNDPVDQLRRFEAQATLAQMGDDEAHG
                     VDADYVRALEYGLPPTGGLGVGIDRLVMLLAGVTSIREVILFPHLRPEADGS"
     gene            2317..>2527
                     /locus_tag="VFX21_11615"
     CDS             2317..>2527
                     /locus_tag="VFX21_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5096658.1"
                     /translation="MRVLVTGMGGELGTRVAQLIEEREWATDIAGVDFVPPRRRLRRA
                     QFRRIDPRDPDRLSAFVEEFAPHAIA"
BASE COUNT          430 a          829 c          830 g          438 t
ORIGIN      
        1 cacagcgtgc gcaccttcga catcggcttg gacattctct aatgctgttg acggttgacg
       61 cgggcaacac gcaaacggtc gtcggcttgt tcaacgaagc cgaactcgtc gaccactggc
      121 gcattgccac ggtcgccgat cgaacctccg acgaactggc gttgatggtg cagcagttcc
      181 tcggcttcca cggcttctcg ttcgacgact ccatcacggg cgtcgcgatc tcgtcggggg
      241 tcccgcgtgt gaccgccgaa ctgcgccaga tgaccgaacg ctattttggc ttcgacgcgc
      301 tcgtcctcga acccggcgtg cgcaccggca tgccgatcct ttacgacaat cccaaagagg
      361 tcggcgcgga ccgcattgcc aacgccgtcg gcgcgtacga cctctacggc gggccgtcga
      421 tcgtcgtcga cttcggcact gccaccacca tcgaggccgt cagcgagcgc ggcgagtaca
      481 tgggtggtgc catctttccc ggcgtcgaga tctcgatgga cgcgctcttc ggtcgcgcgg
      541 ccgggcttcg ccgcgtcgag ctgatcgagc cgaagcacgt catcgggcgg aactcttcgg
      601 agtcgattca gtccggcatg ctctacggct tcagcgctca ggtcgacggg ctggtcgacc
      661 gctttgttgc cgaactcggc ccgtgcgaag tgatcgcgac cggcggcctc gccgaaccca
      721 tcatcgagtt ctcgcgcacc gtgcagcact atgaaccctg gctgacgctc tacggtctgc
      781 ggatcatctt cgaaaggaac cggtgagtcc ggaagcacca agttctgagc ccaacgccga
      841 attccagcag cggctcgcaa aggtcgagga gatccgcgcc cgcggcgacg atccctatcc
      901 ggtcaagttc gaccgtacgc attcgctcgg tgaagtccgg cgcgtctggg acgacaaggt
      961 cgatgtcggc gcgtcgacgg acgacgtcgt tcgcgtcgcg ggccgcgtgt tgctgctgcg
     1021 cgacatgggc aagctcgcgt tcgcgaccgt gcgcgacggc accggcgaca ttcagttctt
     1081 cgtgagtaag ggcgaggtcg gtgacgatgc gttcgcccgc ttcgtcgccg aggtcgatcg
     1141 cggcgactgg gttggcgcga ccggcaccgt catgaagacg aagaagggcg agctgtcggt
     1201 caagatcagc gaattctcgt tgctgtcgaa ggcgctgcgc ccgctgccgg agaagtggca
     1261 cgggctgtcc gataccgaca cgcgctaccg gcagcggtac gtcgacctca tcgcgaacga
     1321 cgacgcgcgg cgggtgttcg acgtgcggtt cacggctgtg aacgcaatcc gccagttcct
     1381 gctcgaacgc ggctatgtcg aggttgaaac cccggtcctt catccgatcg cgggaggggc
     1441 caccgcgcgc ccgttctcga cgcatcacaa cgcgctcgat tccgagctgt tcttgcgcat
     1501 cgcgcccgag ctctacctga agcgactgat cgtcggcggc tacgaccgcg tgttcgaact
     1561 tgcgcgcgtg tttcgcaacg aaggcctgtc gacgcgccac aaccccgagt tcacgatgct
     1621 cgagctctac gaagcgttcg ccgactacca cgacatcgcg cgtctcaccg aggagatgat
     1681 cgcggcggcg gcgcgggccg cgacgggcgg cacgatcgtc gagtgggacg gtgaggaagt
     1741 cgacctgaca ccgccgttcc agcgccgcac gctgctcgaa ctcatcgaag agcacgccgg
     1801 ggtcaaggtg cacccgagtc agccgatcga ggagctgcgc gccgtctgcg accaactcgg
     1861 cgtggcgtgg gacaagaagg accggcccgg gaaactcgtg ctcgagatct acgaaaagac
     1921 gaccgaagcg aacatcaagg gcccgacgtt cgtcatggac tatccgcgcg acgtctcgcc
     1981 gctcgcgcgc gagcaccgcg acgatccgac gatgaccgaa cggttcgaac tcatcgtgcg
     2041 gggccgcgaa ctcgggaacg cgttcagtga gctgaacgat ccggtcgacc aactgcgccg
     2101 cttcgaggcc caggccacgc tggcgcagat gggtgatgac gaggcgcacg gcgtcgacgc
     2161 cgattacgtg cgggcgctcg aatacggcct gccgccgacc ggtggactcg gggttggcat
     2221 cgaccgcctg gttatgttgt tggccggagt gacctcgatc cgggaagtga ttctcttccc
     2281 gcacctgcgc ccggaggccg acggttcgtg agtggcatga gagttctcgt cacgggaatg
     2341 ggcggcgaac tcgggactcg cgtcgcgcaa ctgatcgaag agcgcgagtg ggcgaccgac
     2401 attgcgggcg tcgacttcgt gccgcctcgc cgccggctgc ggcgcgcgca gttccgtcgt
     2461 atcgatccgc gcgatcccga ccggctctcc gccttcgtcg aggagttcgc cccgcacgcg
     2521 atcgcgc
//
LOCUS       DASUGA010000590         4316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_890819, whole genome shotgun sequence.
ACCESSION   DASUGA010000590 DASUGA010000000
VERSION     DASUGA010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890819"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VFX21_11620"
     CDS             complement(<1..445)
                     /locus_tag="VFX21_11620"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5096659.1"
                     /translation="MEPGGLVAAGRDGAIYEFGPGRVLRKTFDGRSIASEARIMQHVA
                     EHGYPVPRIDEVRANGTEIVMERIDGVPMLDDMVRPPTKLPKAMRTLADLHDQLHAIA
                     APDWMRDLDDGGECVVHLDLHPQNVLMTASGPVVIDWANACRGHAL"
     gene            complement(459..2009)
                     /locus_tag="VFX21_11625"
     CDS             complement(459..2009)
                     /locus_tag="VFX21_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX5096660.1"
                     /translation="MWPGRWAAERPEHPAYVMASSGEVVTYARLNDRSNQLAQMFWDA
                     GLRFGDHIAVLMENRVEYMEPCWAAQRSGLFFTCINSHFNAEEVAYILDDCDAQVLVV
                     SSTLREVAEQLVDLMPRVKIRLVVGDPIDGYDRYEEALAKYPAEPLAEELEGTRMLYS
                     SGTTGRPKGVKYKIERKRVGEQPVEMGMMTLVWGMNEDSVYLSPAPLYHSAPLFYSMS
                     TMRLGGTAIIMEHFDPEGALAAIEKYKCTHAQFVPTMFVRMLKLPDAVREKYDTSSQK
                     VVLHAAAPCSVETKRKMIEWWGPIIDEYYAATEGTGATYINSADWLAHPGSVGKSMLG
                     PIHIVDEEGGIQPPGRVGTVWFEPPPNRPGFEYHKDPEKTRDSFNDRGWSTVGDMGYL
                     DEDGYLFLTDRRTFMIVSGGVNIYPQETENLLIDHPKVYDVAVFGIPDPEMGERVHAV
                     VQPVDWNDQGPGLEAELLEYCREHLAHYKCPKAIDFDQELPREPTGKLYKRLLRDRYW
                     GNQTSRIV"
     gene            complement(2014..3096)
                     /locus_tag="VFX21_11630"
     CDS             complement(2014..3096)
                     /locus_tag="VFX21_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEX5096661.1"
                     /translation="MRAAVFTETGGALTIENLEPNPPGPRDVVVQVGASGVCHSDLSL
                     KNGYVPLMPGTVLGHEGAGTVVEVGKEVAHFKKGDHVVAAFIPACGQCWFCLHDESHL
                     CDTEMAVMMSMRGKRPDGNEYMAMTGLGTFAEAMTCNEASLVKINTEVPDEQLALIGC
                     GVTTGVGAALNTAGVTPGSTVAVIGCGGVGQSVIQGARIAGAGRIIAVDPVEMKRKTA
                     EALGATDFVDPSQGDPVAQVQQLTGGRGVDYSFEVIGLPETTLQAYNMIRKGGTAVMV
                     GMTKADAMVTLPTFDLFYNEKTLKGCKYGSGQVRRDFQRFADLIETGRLDTSTMVSQT
                     IKLDQVNDAFDAMEKGEVIRSVITTF"
     gene            3164..3595
                     /locus_tag="VFX21_11635"
     CDS             3164..3595
                     /locus_tag="VFX21_11635"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX5096662.1"
                     /translation="MGATSIPARHVVVITCMDSRIDPLAPFGLEHGDAHILRNGGAVA
                     SDDVLRSLVLSQRLLETTTVHVMAHTDCGLRKIHDEELAKQLTWETGQAPPFAFGAFF
                     DEEAHVREQVARIRDCPWLPHVGDVHGWIYDVSDGSVREVV"
     gene            3608..>4316
                     /gene="pheA"
                     /locus_tag="VFX21_11640"
     CDS             3608..>4316
                     /gene="pheA"
                     /locus_tag="VFX21_11640"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX5096663.1"
                     /translation="MTTAYFGPPGTFTEEALLSQPDLAAGTRVPYASVPEVIAAVEAG
                     DADGGVVPIENMIEGSVSVTLDTLAFDSELLIQREIDLPVTLNLCARPGVKLGDIQTV
                     VSFPHAIAQCRGWLHKKLPKAQSRASHSTSDAAREVSKSKRGDIAAICNSLAAELYGL
                     EVLATDVEDHPENQTRFVLVGRGVPAPSGHDKTSIVCFQRANRPGSLLAILQEFAARA
                     IDLSKLESRPTKRNLGDY"
BASE COUNT          703 a         1454 c         1370 g          789 t
ORIGIN      
        1 acaacgcgtg acctcgacac gcgttggccc agtcgatgac caccggaccg gacgcggtca
       61 tcaacacgtt ctgcggatgc agatcgagat gcaccacaca ttcgccgccg tcgtcgagat
      121 cacgcatcca atcgggtgcc gcgatggcat gcagttggtc gtgcaggtcg gcgagggtgc
      181 gcatcgcttt cggaagtttc gttggcggcc gcaccatgtc gtcgagcatc ggaacgccgt
      241 cgatgcgttc catcacgatc tcggtgccgt tcgcgcgaac ctcgtcgatg cggggaacgg
      301 ggtacccatg ctcggcgacg tgctgcatga tgcgcgcttc gcttgcgatc gaccggccgt
      361 cgaacgtctt gcgcaacacg cgtcccggac cgaactcgta gatcgcgccg tcgcgacccg
      421 cggcaacgag tccgcccggt tccactatct gcgttccatc agacgatgcg gctcgtttgg
      481 ttgccccagt accggtcgcg cagcaagcgc ttgtagagct tgccggtcgg ctcgcgcggc
      541 agctcttggt cgaagtcgat ggccttgggg cacttgtaat gcgcgagatg ctcgcggcag
      601 tactcgagca gctcggcttc gaggccggga ccttggtcgt tccaatccac gggctgcacg
      661 accgcgtgca cgcgttcgcc catctcggga tcgggaatgc cgaacaccgc cacgtcgtac
      721 accttggggt ggtcaatcag caggttctcg gtttcctgcg gatagatgtt cacgccgccc
      781 gacacgatca tgaacgtgcg gcggtcggtg aggaacaggt agccgtcttc gtcgaggtag
      841 cccatgtcgc cgaccgtcga ccagccgcga tcgttgaacg aatcgcgcgt cttctccgga
      901 tccttgtggt actcgaaccc cggacggttg ggcggcggtt cgaaccacac ggtgccgacg
      961 cgccccggag gttgaatgcc gccttcttcg tcgacgatgt ggatcgggcc gagcatcgac
     1021 ttgccgaccg aacccggatg cgcgagccaa tcggccgagt tgatgtacgt cgcgcccgtg
     1081 ccttccgtcg cggcgtagta ctcgtcgatg atcggccccc accactcgat catcttgcgc
     1141 ttcgtctcga ccgagcacgg cgcggccgcg tgcagcacga ctttctgcga ggacgtgtcg
     1201 tacttctcgc gcacggcgtc gggcagcttc aacatgcgca cgaacatcgt cggtacgaac
     1261 tgcgcatgcg tgcacttgta tttctcgatc gcggcgagtg caccctcggg atcgaagtgc
     1321 tccatgatga tcgcggtgcc accgaggcgc atcgtgctca tcgaatagaa cagtggagcc
     1381 gagtgatata gcggcgcggg cgagagatac acggagtcct cgttcatgcc ccagacgagc
     1441 gtcatcatgc ccatctcgac cggttgctcg ccgacgcgct tgcgctcgat cttgtacttc
     1501 acgcccttgg ggcggccggt tgtgcccgac gaatacaaca tccgggtgcc ttcgagctcc
     1561 tcggccaagg gctcggccgg gtacttcgcc agtgcctctt cgtagcggtc gtaaccgtcg
     1621 atcggatcgc ccacgaccag tcgaatcttg acgcgcggca tgaggtccac gagctgctcg
     1681 gctacctcgc gcaacgtcga cgacacgacg agtacctgcg cgtcacagtc gtcgaggatg
     1741 tatgccacct cctcggcgtt gaagtgcgag ttgatgcagg tgaagaacag gccggagcgt
     1801 tgcgcggccc agcacggctc catgtactcg acgcggttct ccatcaacac cgcgatgtga
     1861 tcgccgaagc gcaggcccgc gtcccagaac atctgcgcga gctggttcga ccggtcgttc
     1921 aaccgcgcgt acgtcacgac ctccccggag gaggccatga cgtacgccgg atgttcaggc
     1981 cgttcggcgg cccacctgcc gggccacacg gagttagaag gtcgtgatga ccgagcgaat
     2041 gacttcgccc ttctccattg cgtcgaacgc gtcgttgacc tggtcgagct tgatcgtctg
     2101 cgacaccatc gtcgacgtgt cgagccggcc ggtctcgatg aggtcggcga agcgctggaa
     2161 gtcgcggcgc acctggcccg agccgtactt gcagcccttc aacgtcttct cgttgtagaa
     2221 gaggtcgaac gtcggcagcg tgaccatcgc gtcggccttg gtcatgccga ccatgaccgc
     2281 ggtaccaccc ttgcgaatca tgttgtacgc ctggagggtc gtctcgggca gaccgatgac
     2341 ctcgaacgag tagtcgacac cgcgaccgcc cgtgagctgt tgcacctgcg caacggggtc
     2401 gccctggctc gggtcgacga agtcggttgc gccgagcgcc tcggcggtct tgcgcttcat
     2461 ctcgaccggg tcgaccgcga tgatgcgccc cgcgcccgcg atgcgtgcac cttgaatcac
     2521 cgactggccg acaccaccgc agccgatgac cgcgaccgtc gagccgggcg tgacgcccgc
     2581 ggtgttgagc gcggcaccga caccggtggt gacgccgcag ccgatcagcg cgagctgctc
     2641 gtcgggcacc tcggtgttga tcttcacgag cgacgcctcg ttgcacgtca tcgcctcggc
     2701 gaaggtgccc agcccggtca tcgccatgta ctcgttgccg tcgggccgct tgccgcgcat
     2761 cgacatcatc acggccatct cggtgtcgca caggtgtgat tcgtcgtgca ggcagaacca
     2821 gcactggccg cacgcgggga tgaatgccgc gacgacgtgg tcgcccttct tgaagtgcgc
     2881 gacttccttg ccgacctcga ccacggtgcc ggccccttcg tggccgagca cggtgccggg
     2941 catcagcggc acgtagccgt tcttcagcga gaggtccgag tggcacacgc cgctcgcgcc
     3001 gacttggacg acgacgtcac gcgggccggg cgggttcggc tcgaggttct cgatcgtgag
     3061 cgccccgccc gtttcggtga atacagcagc ccgcatgggc tttcctcctg gcttgacctg
     3121 cgattccttg cgcaacctag cgcccctcct acagttcgca cacgtgggcg ctacctccat
     3181 cccggcccgg cacgttgtcg tgatcacgtg catggactcg cgcatcgatc cgctggcacc
     3241 attcgggctc gaacacggcg acgcccacat cctccgcaac ggaggcgcgg tggcgagcga
     3301 cgacgtgttg cgatcgctcg tgctctccca gcggctcctc gaaacgacga ctgtccacgt
     3361 catggcgcac acggattgcg ggctccgcaa gatccacgac gaagagctcg ccaagcagct
     3421 gacgtgggag acgggccagg ccccgccgtt cgcgttcgga gccttcttcg acgaggaggc
     3481 ccacgtccgg gagcaggtcg cgcgaatccg ggactgcccc tggctcccgc acgtcggcga
     3541 cgtgcacggc tggatctacg acgtatcgga cggctcggtg cgcgaggtcg tttagcctgc
     3601 tctttcagtg accactgcgt atttcggtcc tccgggcaca ttcactgagg aagcgctgct
     3661 gtcgcagccc gacctggccg cgggcacccg cgtcccatac gcatccgttc ctgaggtcat
     3721 cgcggccgtg gaggccggtg acgccgacgg tggcgtggtt ccgatcgaaa acatgatcga
     3781 gggatcggtt tcggtcacgc tcgacacgct ggcgttcgac tccgagctgc tcatccagcg
     3841 cgagatcgac ctgccggtca cgctgaacct gtgcgcgcgc ccgggcgtca agctcggaga
     3901 catccaaacc gtcgtctcgt tcccgcacgc gatcgcgcag tgccggggct ggctgcacaa
     3961 gaagctcccg aaggcgcagt cgcgcgcgtc gcactccact tccgatgcgg cgcgcgaggt
     4021 ctcgaagtcg aaacgcggcg acatcgccgc gatctgcaat tcgctggccg ccgagctgta
     4081 cgggctcgag gtgctcgcga ccgatgtcga agatcatccc gagaaccaga cccgcttcgt
     4141 gctcgtcggc cggggcgtcc ccgcgccgag cggccacgac aagacctcga tcgtctgctt
     4201 ccagcgcgcg aaccggccgg gttcgctcct cgcgatcttg caggagttcg ccgcccgcgc
     4261 aatcgacttg agcaagctcg agtcgcggcc gacgaagcgc aatctcggcg actact
//
LOCUS       DASUGA010000591         5049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_316177, whole genome shotgun sequence.
ACCESSION   DASUGA010000591 DASUGA010000000
VERSION     DASUGA010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316177"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VFX21_11645"
     CDS             complement(<1..872)
                     /locus_tag="VFX21_11645"
                     /inference="COORDINATES: protein motif:HMM:NF033747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HEX5096664.1"
                     /translation="MRRAIAGFGRTLVTLGLLMLLFVGYQLWGTGYFTARAQASLEDD
                     FAQAQADYRAQTADDPVVDTSATTGPTTTTTTVPAPLPIPAEGDVVGGINIEKIDVHK
                     WFVQGTERADLKKGPGHYPETVMPGQLGNAAIAGHRTTYGAPFNRLDELHVGDEIVVT
                     LPNGNYTYKVSQEPFAVKPTETSVVANTPDRATLTLTTCNPKYSARERLVIKADLVEN
                     VSPPVVDPKDLPEASQPVKTESHDAALEDGLQGEQKALGPAWGWGLLVLVVGMGWWWV
                     YRRWRHPAMWALGVV"
     gene            914..1600
                     /locus_tag="VFX21_11650"
     CDS             914..1600
                     /locus_tag="VFX21_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5096665.1"
                     /translation="MLVVSLRSAVCLLGRFPALAGADLDVEDGEILLVAGANGAGKTT
                     LLRCCAGLLPLRTGTLTVLGHDLARDRRAVRRSVALVGHETFCYDDLTVAENLRFAAR
                     AAGRRDVDVAALLERVGLDRQAGVAHGRLSQGQRRRLSLGIALSRDPKLVLLDEPHAG
                     LDTEGRAVVDAVVAAAPAEGRTVLIASHELDLVRTLATREVQIVDGRIFPATAPTTAP
                     RPAPEVEVTP"
     gene            1603..2271
                     /locus_tag="VFX21_11655"
     CDS             1603..2271
                     /locus_tag="VFX21_11655"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX5096666.1"
                     /translation="MLREALLVAGKDLRIELRSRVALQQILPFGGIVVLMFAFALDPD
                     RGLLPRVAPGLFWVAVLLASLLAVSRTFALEEANRARDGLRLSGLDGGAIFLGKAAAI
                     AVQLALLEAILGIGVVALYDVEVREIVLLLATAVVATIGLAAAGTVYGVLASGARARE
                     TLVPLLVLPAVTPIMLGGTRAFEAALDGVPADGWPWVQLLAAFGVLALVAGTIAFGPL
                     LEED"
     gene            2274..2990
                     /gene="ccsA"
                     /locus_tag="VFX21_11660"
     CDS             2274..2990
                     /gene="ccsA"
                     /locus_tag="VFX21_11660"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX5096667.1"
                     /translation="MRLRWLGVLSVLALLGLGYFALVAAPPDAEQGDAYRLLYLHVPC
                     AWLAYLAFFVTFVCSVLWLVPRTRSATWDLLAGASAQVGVLFTGLTLIMGSIWGRPIW
                     GTWWEWDARLTTTIVLFFLYLGYLALRRTGSTATQRGKVCAIAAIIAFVDVPIVHFSV
                     TWWQTLHQDGSVFNDKMDVGLEGFWMQFTLLYSVFAFTLLYVFLVLARFQLAEMEEGA
                     EERELQRAIDARIHQQKVLV"
     gene            2993..3130
                     /locus_tag="VFX21_11665"
     CDS             2993..3130
                     /locus_tag="VFX21_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096668.1"
                     /translation="MDHNWGYVAGGYIITTVTLLVYGAWIWRRTRRARRTMPSVDLDA
                     G"
     gene            3141..3551
                     /locus_tag="VFX21_11670"
     CDS             3141..3551
                     /locus_tag="VFX21_11670"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX5096669.1"
                     /translation="MIAAVVCAGVVVFMLTVLQKNVVYLKPVSEAVSQREQQGDRSFR
                     MGGTVVAGSITEPDGGGADFVLTEGGENARVQYSGSPPDLFKDCAPVVVEGHWQGTTF
                     VTNRLLIRHGNEYKPPSDDKAADANIDCAELASK"
     gene            3548..>5049
                     /gene="ccsA"
                     /locus_tag="VFX21_11675"
     CDS             3548..>5049
                     /gene="ccsA"
                     /locus_tag="VFX21_11675"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX5096670.1"
                     /translation="MRSAVGYFLLVLGFASAVAGISTLLAGILKERPQLLRFGRECVF
                     IAFAAAVGAFVTMETALFAHDYSIKFVAENVANGTPGLYTFTAAWSALQGSILLWALA
                     LTGYLAVTTWRFRKRANDPIVAWATLVALCVVLFFFALMIFTDANPFTEASTVVADGR
                     GPNPLLQNHPLVAFHPPMLYLGYVGFTIPFAFAIAALITGRFGEGWLADVRRTTLIAW
                     GFLTVGIVLGAWWSYEVLGWGGYWGWDPVENASLLPWLTGTAFIHSVMVQERRGMLRV
                     WNLSLVIATFCLTILGTFLTRSGVVNSVHAFSDSNIGTWLLLFLGAVAFTGIGLIAWR
                     GDKLRSPGRIDSPVSREAAFLLNNLLFAGFALVVLTGTVFPLLAEAFQDRQLTVGEPY
                     FERMGVPIGIALLFFMAVGPLLPWRATSGEVMRQRLLIPAWVGGLTFVGLVIVGANGV
                     AQVFAFSLAAFALTSIGRTVVIGVRARRRAHAEGVPTAVVRMVRGNTRLYG"
BASE COUNT          638 a         1722 c         1762 g          927 t
ORIGIN      
        1 acgacgccga gcgcccacat cgccggatgc cgccagcggc ggtagaccca ccaccatccc
       61 ataccgacga cgagcacgag caggccccag ccccacgcgg gcccgagcgc cttctgctcg
      121 ccctggagtc cgtcttcgag cgcggcgtcg tgtgactcgg tcttgaccgg ttgtgatgcc
      181 tccggcaggt ccttggggtc gacgacgggc ggcgagacgt tctcgacgag gtcggccttg
      241 atgacgagac gttcgcgcgc ggagtacttc gggttgcacg tcgtgagcgt cagcgttgcc
      301 cggtcaggcg tgttcgcgac gacgctcgtc tcggtgggct tgaccgcgaa cggctcttgg
      361 ctgaccttgt acgtgtagtt cccgttcggc aacgtgacga cgatctcgtc accgacgtgg
      421 agctcgtcga gccggttgaa cggcgcgccg taggtggtgc ggtgccccgc gatcgccgcg
      481 ttgccgagct ggcccggcat cacggtctcg gggtagtggc cgggaccctt cttgaggtcg
      541 gcgcgttcgg tgccctgcac gaaccacttg tgcacatcga tcttctcgat gttgatgccg
      601 ccgaccacgt cgccctcggc cgggatcggc agcggtgcgg gcacggtcgt cgtggtcgtc
      661 gtcgggcccg tggtggcact ggtgtccacg accgggtcgt cggcggtctg ggcccggtag
      721 tcggcctggg cctgcgcgaa gtcgtcctcg agactggcct gggcccgagc ggtgaagtag
      781 ccggtgcccc agagctggta gccgacgaac aggagcatca gcagacccag cgtcacgagg
      841 gtccgcccga acccggcaat cgcacggcgc atcgatcgag gataccgccg ctgcggccgt
      901 acactcacgc ggcgtgttgg tcgtgagcct gcgatcggcc gtctgcttgc tcggtcgctt
      961 cccggcgctc gcgggcgccg atctcgacgt cgaggacggc gagatcctgc tcgtggccgg
     1021 cgcgaacggc gcgggcaaga cgacgctgtt gcgctgctgc gcgggcctct tgccgttgcg
     1081 caccggcacc ctcaccgtcc tcggccacga tctcgcgcgc gaccggcgcg ccgttcgccg
     1141 ttccgtcgcc ctcgtcggtc acgagacgtt ctgttacgac gacctcaccg ttgccgagaa
     1201 cctccgcttc gcggcccgcg ccgcggggcg gcgcgacgtc gacgtcgccg cgctgctcga
     1261 acgggtgggg ctcgaccgcc aagcgggcgt ggcgcacggc cgcctctcgc agggtcagcg
     1321 ccgccggctg tcgctcggca tcgcgctgag ccgcgacccg aagctcgtgc tcctcgacga
     1381 accccacgcc ggcctcgaca ccgaagggcg cgccgtcgtc gacgccgtcg tcgcggccgc
     1441 gccggccgaa gggcgcacgg tcttgatcgc gtcgcacgag ctggatctcg tccgcacgct
     1501 tgccacacgc gaggtgcaga tcgtcgacgg ccgcatcttc ccggcgaccg cgcccacgac
     1561 ggcgccgcgc ccggcacccg aagtcgaggt cacgccgtga cgatgttgcg cgaggcactc
     1621 ctcgtcgcag gtaaggacct tcgcatcgag ctgcgctcac gcgtcgcgct ccagcagatc
     1681 ctgccgttcg gcggcatcgt cgtgctgatg ttcgcgttcg cgctcgaccc cgaccgcgga
     1741 ctgctcccgc gggtcgcgcc cggtctgttc tgggtcgcgg tgttgctcgc gtcactgctc
     1801 gcggtcagcc gcacgttcgc gctcgaagag gcgaaccgcg cccgcgacgg gctgcgcctc
     1861 tccgggctcg acggcggcgc gatcttcctc ggcaaggcgg ccgccatcgc ggtgcaactc
     1921 gcgctgctcg aggcgatcct cgggatcggg gtcgtggcgc tgtacgacgt cgaggtccgg
     1981 gagatcgtcc tgctgctcgc gaccgcggtc gtcgcgacca tcgggttggc cgccgccggt
     2041 acggtctacg gcgtgctcgc ctcgggagcc cgggcccggg agacgctcgt ccccctgctc
     2101 gtgctgccgg cggtcacacc gatcatgctg ggcgggacgc gagcgttcga ggccgccctc
     2161 gacggcgtgc cggccgacgg ctggccgtgg gtgcaactgc tcgccgcgtt cggagttttg
     2221 gccctcgtcg cgggcaccat tgcattcggc ccgctgctgg aggaggacta ggcgtgcggc
     2281 tgaggtggtt gggagtgctg agcgtgctgg ccctgctcgg gctgggctac ttcgcgctcg
     2341 tcgccgcccc gcccgacgcg gagcagggtg acgcgtaccg actcctctat ctccacgtgc
     2401 cgtgcgcgtg gctcgcctac ctcgcgttct tcgtcacctt cgtgtgctcg gtgctgtggt
     2461 tggtcccgcg cacgcggtcc gcgacctggg acctcctcgc gggtgcgtcc gcgcaggtcg
     2521 gcgtgctctt caccgggctc actctcatca tgggcagcat ctggggccgc ccgatctggg
     2581 gcacctggtg ggagtgggac gcgcgcctga cgacgacgat cgtcctcttc ttcttgtacc
     2641 tcggatacct cgcgctgcgg cgcacaggct cgaccgcaac ccaacgcggc aaggtctgcg
     2701 ccatcgccgc gatcatcgcg ttcgtcgacg tcccgatcgt gcacttctcg gtcacctggt
     2761 ggcagacgct gcaccaggac ggctcggtgt tcaacgacaa gatggacgtc gggctcgaag
     2821 gattctggat gcagttcacg ctgctctaca gcgtgttcgc gttcacgctt ctctatgtgt
     2881 tcctggtgct ggcccgcttc cagctcgccg agatggaaga aggcgccgag gaacgcgaac
     2941 tgcagcgcgc gatcgacgcg cgcatccatc agcagaaagt gctcgtctag tcatggatca
     3001 caactggggc tacgtcgcgg gcggttacat catcacgacc gtcacgttgc tcgtgtacgg
     3061 cgcgtggatc tggcggcgca cgcgtcgcgc gcgccggacg atgcccagcg tcgacctcga
     3121 tgcggggtaa ggccaggtac gtgatcgcgg cggtcgtctg tgccggcgtt gtcgtgttca
     3181 tgctcaccgt gttgcagaag aacgtcgtgt acctgaagcc ggtctccgaa gccgtgtcgc
     3241 aacgtgaaca gcagggcgac cgcagtttcc ggatgggcgg caccgtcgtc gcgggctcga
     3301 tcaccgagcc cgacggtggg ggcgccgact tcgtgctgac cgagggcggc gagaacgcgc
     3361 gcgtgcagta cagcggttct ccgccggacc tgttcaagga ctgcgcgccc gtcgtcgtcg
     3421 agggccattg gcagggcacg acgttcgtca cgaaccggct gctgatccgg cacggcaacg
     3481 agtacaagcc gccgagtgac gacaaggcgg ccgacgcgaa catcgattgc gcggagctcg
     3541 cttccaaatg agatccgcag tcgggtactt cctgctcgtg ctggggttcg cgagcgcggt
     3601 ggcggggatc tcgacgctgc tggccggcat cctgaaggag cggccccagc tcctgcgctt
     3661 cgggcgcgag tgcgtgttca tcgcgttcgc ggcggcggtc ggcgcgttcg tcacgatgga
     3721 gaccgcgctg ttcgcgcacg actactccat caagttcgtc gcggagaacg tcgcgaacgg
     3781 cacaccgggt ctctatacct tcaccgccgc gtggtcggcg ttgcagggct cgatcctgct
     3841 gtgggcgctc gcgttgacgg gatacctcgc ggtgacgacg tggcggttcc gcaaacgcgc
     3901 gaacgatccg atcgtggcgt gggcaacgct cgttgcattg tgcgtcgtcc tgttcttctt
     3961 cgcgttgatg atcttcaccg acgccaaccc gttcaccgag gcgtcgacgg tcgtcgccga
     4021 cggtcgcggg cccaacccgt tgctgcagaa ccacccgctg gtcgcgttcc acccgccgat
     4081 gctgtatctc ggctacgtcg ggttcacgat cccgttcgcg ttcgcgatcg cggcgctgat
     4141 cacgggtcgc ttcggcgagg gctggctcgc ggacgtccgc cgcacgacgc tgatcgcgtg
     4201 gggcttcctc acggtgggca tcgtgctcgg cgcgtggtgg agctacgaag tgctcgggtg
     4261 gggcggctac tggggctggg acccggtcga gaacgcgtcg ttgctgccgt ggctgaccgg
     4321 caccgcgttc atccactccg tgatggtgca agaacggcgc ggcatgctgc gcgtctggaa
     4381 cctgtcgctc gtcatcgcga cgttctgcct cacgatcctc ggcacgttcc tcacgcgctc
     4441 gggtgtcgtg aactccgtgc acgcgttctc ggactcgaac atcggcacct ggttgctgct
     4501 gttcctcggc gccgtcgcgt tcaccggcat cgggctcatc gcgtggcgcg gcgacaagct
     4561 ccgctcgccc ggccgcatcg actctccggt ctcacgtgaa gccgcgttct tgttgaacaa
     4621 cctgttgttc gcgggctttg cgctcgtcgt gctcaccggc accgtgttcc cgctgctcgc
     4681 cgaggcgttc caagaccggc agctgacggt cggtgagccg tacttcgagc gcatgggtgt
     4741 gccgatcggc atcgcgctct tgttcttcat ggcggtcggg ccgttgctgc cgtggcgggc
     4801 gacgagcggt gaggtcatgc gccaacgact gctcatcccg gcgtgggtgg gcgggctcac
     4861 gttcgtcggg ctcgtgatcg tcggtgccaa cggtgtcgcg caggtcttcg cgttctcgct
     4921 cgccgcgttc gcgctgacga gcatcggccg cactgtcgtc atcggcgtgc gggcgcggcg
     4981 ccgggcgcac gcggaaggcg tgcccacggc ggtcgtgcgg atggtgcggg gcaacacccg
     5041 tttgtacgg
//
LOCUS       DASUGA010000592         2618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_510030, whole genome shotgun sequence.
ACCESSION   DASUGA010000592 DASUGA010000000
VERSION     DASUGA010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510030"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="VFX21_11680"
     CDS             <1..1008
                     /locus_tag="VFX21_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020523386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX5096671.1"
                     /translation="LQPLLVGADLQRPAAVEQLRVLAGRVDVPFYSEATDPVAVARNA
                     IEEANRLGRNIVIVDTAGRLQIDQDLMDELRHIRDAVQPQNTLLVVDAMTGQEAVNVA
                     ESFHETVGIDGVILTKIDGDARGGAALSVKEVVGKPILFAGTGEKLDEFEPFHPDRMA
                     GRILGMGDVLTLIEKAETTFDDAEKQRAADILESGRITLEDFLEQMQQVKKMGSIQNV
                     LGMLPGMPKEVKNAELDDREIGRVEAIIRSMTPEERRKPELINGSRRSRIAKGSGTTT
                     QEVNNLLKQFKQVQQMMKTMPGAQRMMKKKGKKGKKGKGPKMPRGLPQGGLAGFPGLP
                     Q"
     gene            1056..1472
                     /gene="rpsP"
                     /locus_tag="VFX21_11685"
     CDS             1056..1472
                     /gene="rpsP"
                     /locus_tag="VFX21_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011014848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX5096672.1"
                     /translation="MAVKIRLMRVGKKKQPTYRVVVADSRSPRDGRFIEIIGQYAPRQ
                     EPSTVTVNNERALHWLNNGAQPTESAAKILEISGVWDEYKQAAGKVAAAKPKAKTPKA
                     KTVKEKPAAEAPPAAEAQAPEEAPAEETPAEEEAAE"
     gene            1475..1804
                     /locus_tag="VFX21_11690"
     CDS             1475..1804
                     /locus_tag="VFX21_11690"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX5096673.1"
                     /translation="MPDDDFDDDDFEEDDDDFEDEVGGEGNRVSGSRARDAVELIARA
                     LVDDPDGVFVDASERGDTVTLLVHASPGDLGRIIGKRGRVIQSVRQVARAAGSTEGVR
                     VTVDVAE"
     gene            1808..2266
                     /gene="rimM"
                     /locus_tag="VFX21_11695"
     CDS             1808..2266
                     /gene="rimM"
                     /locus_tag="VFX21_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481117.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX5096674.1"
                     /translation="MLEVGRIGRAHGLHGEVSVTAITNRDERFAPGSVLHAGPRELVV
                     ASSRRHQDRWLVRFEGIDDRTAAELLRNLVLEAEPIDGDDDELWVHELVGAEVRDKTG
                     TAVGRVTAVEANPAHDLLVLENGALVPAVFVVEQRDGVVIIDPPEGLFDL"
     gene            2266..>2618
                     /locus_tag="VFX21_11700"
     CDS             2266..>2618
                     /locus_tag="VFX21_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX5096675.1"
                     /translation="MRVDVFTIFPEYVDGPLSLALVGKARTAGILDLRLHDPRDATSD
                     PHRSIDDRPFGGGAGMVLMPGPLFASVEAVQPQRPLFLLSASGRPFDQGVARELAASD
                     GFSLICGRYEGVDQRV"
BASE COUNT          485 a          833 c          909 g          391 t
ORIGIN      
        1 ctgcagccgt tgctcgtcgg tgccgacctg caacgtccgg ccgccgtcga gcaactgcgc
       61 gtgctggccg gtcgcgtcga cgtgccgttc tattcggaag cgacggatcc ggtggcggtc
      121 gcgcgcaacg cgatcgaaga ggcgaatcgc ctcggccgca acatcgtgat cgtcgacact
      181 gcgggccggt tgcagatcga ccaagacctg atggacgagc tgcgccacat ccgcgacgcg
      241 gtgcagccgc agaacacgtt gctcgtcgtc gacgcgatga ccggtcaaga agccgtcaac
      301 gtcgcggagt cgttccacga gaccgtcggg atcgacgggg tgatcctcac caagatcgac
      361 ggcgacgcgc gaggtggcgc cgcgttgtcg gtcaaggaag tcgtcggcaa gccgatcctg
      421 ttcgccggta ccggtgagaa gctcgacgag ttcgagcctt tccaccccga ccggatggcg
      481 ggccgcattc tcggcatggg cgacgtgctc acgctgatcg agaaggccga aacgaccttc
      541 gacgacgccg agaagcagcg cgcggccgac attctcgaga gcgggcgcat cacgctcgag
      601 gacttcctcg agcagatgca gcaagtgaag aagatgggct cgatccagaa cgtgctcggg
      661 atgctgcccg gcatgcccaa agaggtgaag aacgccgagc tcgacgaccg tgagatcgga
      721 cgcgtcgagg ccatcattcg ctcgatgacg ccggaagagc gccgcaagcc ggaactcatc
      781 aacggctcgc ggcggtcgcg catcgcgaag ggcagcggaa cgacgaccca agaggtcaac
      841 aacctgctga agcagttcaa gcaggtccag cagatgatga agacgatgcc cggcgcccag
      901 cggatgatga agaagaaggg caagaagggg aagaagggca agggtccgaa gatgccccgc
      961 ggcctgcccc agggcggttt ggccggtttt cctggccttc cccagtagtt gccctagact
     1021 gccccggttc tcaggtctcg tcaggagctt tcgttttggc agtcaagatt cgtctgatgc
     1081 gcgtcggcaa gaagaagcag ccgacctacc gcgtggtcgt cgccgactcg cgcagcccga
     1141 gggacgggcg gttcatcgag atcatcggcc agtacgcgcc gcgccaggag ccgtcgaccg
     1201 tgacggtgaa caacgagcga gcgctgcact ggttgaacaa cggcgcccag ccgaccgaaa
     1261 gcgcggcgaa gatcctcgag atctccggcg tctgggacga gtacaagcag gcggcgggca
     1321 aggttgccgc ggccaagccg aaggccaaga cgccgaaggc gaagacggtg aaggaaaagc
     1381 cggcggccga agcgccgccc gcagccgagg ctcaggcgcc cgaagaagcc cccgccgagg
     1441 agacgccggc cgaagaagaa gcggccgaat agtcatgccc gacgacgact tcgacgacga
     1501 cgacttcgag gaagacgacg acgacttcga agacgaagtt ggcggcgaag gcaaccgggt
     1561 ttcgggttcg cgggcgcgcg acgcggtcga attgatcgca cgggcgctgg tcgacgaccc
     1621 ggacggcgtg ttcgtcgacg cctccgagcg gggcgacacc gtcacactgc tggttcacgc
     1681 gagcccgggc gacctgggtc gcattatcgg caagcgcggg cgcgtcatcc aatcggtccg
     1741 tcaggtcgcg cgtgcggccg gctcgaccga aggcgtgcgc gtcaccgtcg acgtcgcgga
     1801 atagctcttg ctcgaagtcg ggcgcatcgg tcgcgcgcac ggcttgcacg gcgaagtctc
     1861 ggtcaccgcg atcacgaatc gcgacgaacg cttcgcgccc ggttcggtgc tccacgccgg
     1921 cccgcgtgag ttggtcgtcg cgtcgtcgcg gcggcaccag gaccggtggc tcgttcgctt
     1981 cgaagggatc gacgaccgca ccgccgcgga gctgctgcgg aacctcgtgc tggaagcgga
     2041 gccgatcgac ggcgacgacg acgagctttg ggtgcacgag ctcgtcggcg cggaagtgcg
     2101 cgacaaaacc ggcactgccg tcggacgggt gactgcagtg gaagcgaacc ccgcgcacga
     2161 cctcttggtg ctcgagaacg gcgcgttggt gcccgccgtc ttcgtggtcg aacagcgcga
     2221 cggcgttgtg atcatcgacc cgcccgaagg actcttcgat ctctgatgag agtcgatgtg
     2281 ttcacgatct ttccggagta cgtcgacggt ccgctgtcgc tcgcgctcgt cgggaaggcg
     2341 cgcaccgccg gcattctcga tctgcggctg cacgatccgc gcgacgcgac atccgacccg
     2401 caccgttcga tcgacgaccg gccgttcggc ggcggcgccg ggatggtgct gatgcccggc
     2461 ccgctgttcg cgtcggtcga agcggtgcaa ccccaacggc ccctgttctt gttgtccgcg
     2521 agcggccggc cgttcgatca aggcgtcgcg cgcgaacttg ccgcgagcga cggcttctcc
     2581 ttgatctgcg gccgctacga aggtgtcgat cagcgcgt
//
LOCUS       DASUGA010000593         3307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1659048, whole genome shotgun sequence.
ACCESSION   DASUGA010000593 DASUGA010000000
VERSION     DASUGA010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659048"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VFX21_11705"
     CDS             complement(<1..258)
                     /locus_tag="VFX21_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018352828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX5096676.1"
                     /translation="MRQNRVLTIPNVISVVRLAFVPVFLWLLWGKDEPIAAAGLLAFL
                     GATDWVDGYIARHFDQGSELGKVLDPTADRVLLVAAAVALLA"
     gene            complement(270..1832)
                     /locus_tag="VFX21_11710"
     CDS             complement(270..1832)
                     /locus_tag="VFX21_11710"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEX5096677.1"
                     /translation="MSVWLDEARSYPSGVVATPHYLATAAGAAMLADGGNAVDAALSA
                     NLVLAVVTPYMCGFGGDLLTMVWDGAVHAYRGVGRAPAAATLDAVRDRSAADAMPVFG
                     PHPVTVPGAIDGWFTMLGKWGTRSFGEVATTALRYAEDGFAVTKRGAWFFARCAQMFD
                     AFDLPDFRNAYGDVRGGDWVRQPALAKTMRRLADDGPDAYYRGPIGEAIAARVQAAGG
                     FMTVEDVRAHAGAWVEPLRATVRGTEILEMPPPTQGMTALEALRITDGLIDGADGPDR
                     QHLLIEAVKLALADRHEYLGDPDAMTTDPQHILADDWIAARRATIDPHRAQHPAPYQG
                     PDGGTIYMCTADRDGMLVSLIQSNFTAAGSGLRVDEWGINLHNRGSAFTFADGHPNAI
                     GAGKMPLHTLIPALALRDGRPWLVFGTEGGHGQAQTHTQLLTRMLVDGADPQAAISAP
                     RFTIDPGSWRVTAEDHFDPAWIAALRERGHDIDVVKGYRHGPGIAHAIECLHPGYRAA
                     SDPRAEGGTSGL"
     gene            1850..>3307
                     /locus_tag="VFX21_11715"
     CDS             1850..>3307
                     /locus_tag="VFX21_11715"
                     /inference="COORDINATES: protein motif:HMM:NF005863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX5096678.1"
                     /translation="MAFNLADLLEHVTDAVPDRVAIVAGDRRLTFQQLDERANRFANY
                     LRDVGVRPGDHVGIYAFNRAEWVEAMFGCFKARAVPINVNYRYVPDELRYLFDNADIV
                     ALVYEAEFGPHVASVLPGLPKLQHLLELEDGTGTSSPELGAVDYEQALASASPERSGE
                     ERSADDLYILYTGGTTGMPKGVMWRQEDIFFAAMGGGNYGGAGIDAPEEIGGNVSPHP
                     GVSMALAPLMHGNAQWTMSIALFGGNTVVLNTARTFDALQIWDLVEREGVSVISLVGD
                     AMARPLVDALASRPIPPTVFAIVSGGAILSPAIKGQINDVAPNVMVMDAFGASETGAN
                     GAVDVTDKGPRFRMNEWTTVITEDGKQAEVGEIGLLARRGHGPIGYYKDEAKTAATFV
                     EFDGVRWAIPGDRAVIEEGGVVNVLGRGSQCINSGGEKIFPEEVEAVLKSHPDVFDAI
                     VVGVPDERWGERVCAVVAPRAGKQPTLDELTKHAET"
BASE COUNT          520 a         1146 c         1160 g          481 t
ORIGIN      
        1 cgcgagcagc gcgaccgccg ccgcgacgag cagcacgcgg tccgcggtcg ggtcgagaac
       61 cttgccgagc tcgctgccct ggtcgaaatg gcgggcgatg tacccgtcga cccagtcggt
      121 ggcgccgagg aacgcgagca gccccgccgc cgcgatcggc tcgtccttgc cccacagcaa
      181 ccagaggaac acggggacga acgccagccg tacgaccgag atcacgttcg ggatcgtcag
      241 cacccggttc tgtcgcaccg ccaccgaaat tacaggccgg acgtcccgcc ctccgcccga
      301 ggatcggacg ccgcccggta gccggggtgc aggcattcga tcgcgtgggc gatgccgggg
      361 ccgtggcggt agcccttcac cacgtcgatg tcgtggccgc gctcgcggag cgcggcgatc
      421 cacgccggat cgaagtggtc ctcggccgtc acccgccacg agccgggatc gatggtgaag
      481 cggggcgccg aaatcgcggc ttgggggtcg gcgccgtcga cgagcatgcg cgtcagcagc
      541 tgcgtgtgcg tttgggcctg cccgtggccg ccctcggtgc cgaacacgag ccacggtcgg
      601 ccgtcacgca acgcgagcgc gggaatcaac gtgtgcaacg gcatcttgcc cgcgccgatc
      661 gcgttcgggt gcccgtcggc gaaggtgaac gcggagccgc ggttgtgcag gttgatcccc
      721 cattcgtcga cgcgcagtcc cgaacctgcg gcggtgaagt tcgactggat gagactcacg
      781 agcatcccgt cgcggtcggc ggtgcacata tagatcgtgc cgccgtcggg accctggtaa
      841 ggcgcggggt gctgcgcgcg gtggggatcg atggttgcgc ggcgggccgc gatccagtcg
      901 tcggcgagga tgtgctgcgg atccgtcgtc atcgcgtcgg ggtcgccgag gtactcgtgg
      961 cggtccgcga gcgcgagctt caccgcttcg atgagcagat gctggcgatc cgggccgtcg
     1021 gcgccgtcga tcagcccgtc ggtgatgcgc aacgcctcca gcgcggtcat gccctgcgtc
     1081 ggcggcggca tctcgaggat ctctgtgccg cgcacggtcg cgcgcagcgg ttcgacccac
     1141 gcgcccgcgt gggcgcgcac gtcttcgacg gtcatgaagc cgcccgcggc ttgcacccgg
     1201 gccgcgatgg cctcgccgat cgggccgcgg taatacgcgt ccggtccgtc gtcggcgagc
     1261 cggcgcatcg tcttggcgag cgcgggctgg cgcacccagt cgccgccgcg cacgtcgccg
     1321 tacgcgttgc ggaagtcggg caggtcgaag gcgtcgaaca tctgcgcgca acgagcgaag
     1381 aaccacgcgc cgcgcttggt cacggcgaag ccgtcttcgg cgtagcgcag cgccgtcgtc
     1441 gccacttcgc cgaacgaacg ggtcccccac ttgccgagca tcgtgaacca gccgtcgatc
     1501 gcgccgggca cggtgacggg atgcggaccg aacaccggca tcgcgtccgc ggccgaccgg
     1561 tcgcgcaccg catcgagcgt cgccgccgcc ggggcccggc cgacaccgcg gtacgcgtgc
     1621 accgcgccgt cccacaccat cgtcagcagg tcgccaccga acccgcacat ataaggagtg
     1681 acgacggcca ggacgaggtt ggcggagagg gcggcgtcga ccgcgttgcc gccgtcggcc
     1741 agcatcgcgg cgcccgcggc cgtcgccagg tagtgcggcg tggccacgac accgctcgga
     1801 tacgacctcg cctcgtccag ccagacgctc atccgaatat gtttatcgaa tggctttcaa
     1861 cctcgccgac ctgctggaac acgtgaccga cgcggtcccc gaccgggtcg ccatcgtcgc
     1921 cggtgaccgc cggctcacgt tccagcagct cgacgagcgc gcgaaccgct tcgcgaacta
     1981 cctccgcgac gtcggtgtgc gccccggcga tcacgtcggc atctacgcgt tcaaccgcgc
     2041 cgaatgggtc gaggcgatgt tcggctgctt caaggcgcgg gcggtgccga tcaacgtcaa
     2101 ctaccgctac gtgcccgacg agctgcggta cctgttcgac aacgcggaca tcgtcgcgct
     2161 cgtctacgaa gccgagttcg gtccgcacgt cgcgtcggtg ctgccgggcc tgccgaagtt
     2221 gcagcacctc ctcgagttgg aggacggcac cggcacgtcc agtcccgagc tcggtgcggt
     2281 cgactacgag caggccttgg cgagcgcgtc ccccgaacgt tcgggtgagg aacgctcggc
     2341 cgacgatctc tacatcctct acaccggcgg caccaccggc atgcccaagg gcgtgatgtg
     2401 gcgtcaggaa gacatcttct tcgcggcgat gggcggtggg aactacggcg gcgcgggcat
     2461 cgacgccccc gaggagatcg gcggcaacgt ctcaccgcac cccggcgtgt cgatggcact
     2521 cgcgccgttg atgcacggca acgcgcaatg gacgatgagc atcgcgctgt tcggcggcaa
     2581 caccgtcgtc ttgaacaccg cgcgcacctt cgacgcgctc cagatctggg acctcgtcga
     2641 acgcgagggc gtctccgtga tcagtctggt cggcgacgcg atggcgcggc cgttggtcga
     2701 cgcgctcgcg tcgcgcccca tcccgccgac cgtgttcgcg atcgtgtcgg gtggcgcgat
     2761 cctctccccc gcgatcaagg gccagatcaa cgacgtcgca ccgaacgtga tggtgatgga
     2821 cgcgttcgga gcgtcggaga ccggcgcgaa cggcgcggtc gacgtcaccg acaagggccc
     2881 gcgcttccgc atgaacgagt ggacgacagt catcaccgaa gacggcaagc aggccgaggt
     2941 cggcgagatc gggttactgg cgcggcgcgg ccacggtccc atcgggtact acaaggacga
     3001 agcgaagacc gcggcgacat tcgtcgagtt cgacggcgtg cgctgggcga tcccgggcga
     3061 ccgcgcggtg atcgaagaag gcggggtcgt caacgtcctc ggccgcggtt cgcagtgcat
     3121 caacagcggt ggcgagaaga tcttccccga ggaagtcgaa gctgtgctca agagccaccc
     3181 cgatgtcttc gacgcgatcg tggtcggggt gcccgacgaa cgttggggcg agcgcgtgtg
     3241 cgcggtcgtc gcgccgcgcg ccggcaagca accgaccctc gacgagctca ccaagcacgc
     3301 cgaaacc
//
LOCUS       DASUGA010000594         5537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_994644, whole genome shotgun sequence.
ACCESSION   DASUGA010000594 DASUGA010000000
VERSION     DASUGA010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994644"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VFX21_11720"
     CDS             <1..340
                     /locus_tag="VFX21_11720"
                     /inference="COORDINATES: protein motif:HMM:NF036848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEX5096679.1"
                     /translation="WYLTKHGVGVWSATPPTRPFALAADTQARVDALPHCEAAGLVEG
                     DATIEATSVAFERDGTPSSGLVALRLDERRRALARCTDPDLLRAMTESTWEGRSVTVR
                     NDGSTNTIAG"
     gene            350..562
                     /locus_tag="VFX21_11725"
     CDS             350..562
                     /locus_tag="VFX21_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096680.1"
                     /translation="MADQLDVDAMLERFRQRAAAVKDRPLPPVAGNERQMFIEQAQLD
                     FQDFAIIGDATWTFENGVLILKVDLS"
     gene            complement(613..1017)
                     /locus_tag="VFX21_11730"
     CDS             complement(613..1017)
                     /locus_tag="VFX21_11730"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEX5096681.1"
                     /translation="MAEQPSRFTTLWRGFTKRCARCGSGKLFRRWFVMVPDCPRCGLH
                     FEREQGYWTGALAFNMIMTGGLFAVAFVVTLIVQLPDVEPVELLAVCIPIVALGPIIL
                     YPFSKTVWVAVDRAFLQRLDVNERPDEQMRRY"
     gene            complement(1028..1921)
                     /gene="prmC"
                     /locus_tag="VFX21_11735"
     CDS             complement(1028..1921)
                     /gene="prmC"
                     /locus_tag="VFX21_11735"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX5096682.1"
                     /translation="MKWRTLRAAVAHRLRDAGIESPDTEARFITEECSGHAGGEWLEI
                     EDTDAIARAEARVHAMCERRAAGEPLQYVLGSWAFRDLDLMVDSRVLIPRPETEIVVE
                     VALDEAVRLGLRRAKARGAVPSAPTAVVADLGTGSGAIALALEHELPEVEVWATDASE
                     DALAVARANLAGCGATRVRVAPGSWFDALPRELRGWLTLVVSNPPYVAQHEVIALPPV
                     VLDYEPRTALVAGPTGLEALTHLVDEAPAWLAPRGALVAELAPHQAADMVPYARAAGF
                     DDVTIRPDLTGRDRVLVARRG"
     gene            complement(1918..2997)
                     /gene="prfA"
                     /locus_tag="VFX21_11740"
     CDS             complement(1918..2997)
                     /gene="prfA"
                     /locus_tag="VFX21_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX5096683.1"
                     /translation="MFEQLEDLEQELERLEAELPGIYASGDRRASRDAGRRHAELKPL
                     VGAYQEWRAAGNDVAEAQELLEGESDADMREFLKGEIAEKQARVDELAEQIRELLLPR
                     DPNEGRNVIVEIQGTEGGEEANIWAGDLFRMYQRFADRNGLKLEVLDQQVSEHGGFRD
                     ITFAVKGDAAWGRFKYEGGPHRVQRVPVTESQGRVHTSAATVAVLPEAEEVDIEIDPN
                     DLEIDVFRSSGPGGQSVNTTDSAVRMTHKPTGLVVSCQDEKSQLQNKDKALRILRSRL
                     LQLEQERQAAELSAARKGQVKTGGRSEKIRTYNYKENRVTDHRIPGLTLHALDRVLAG
                     ERELDEIIDRLGEDERARQLAGEVS"
     gene            3200..3520
                     /locus_tag="VFX21_11745"
     CDS             3200..3520
                     /locus_tag="VFX21_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096684.1"
                     /translation="MHAQADARVAAEVSTSTHEVQRWAWFDLNGDGTIDDTSPLYGGD
                     GVLVWNGSGAKPSHPRPAEQSTSTRSSDEHSTPVKVEHARAAYTRYGQASPTHESAPH
                     AAVA"
     gene            3758..4552
                     /locus_tag="VFX21_11750"
     CDS             3758..4552
                     /locus_tag="VFX21_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096685.1"
                     /translation="MLGLEERPSADIPVIRPVKRSSGPVIGLALGLVVVLIAGVWFVT
                     NRDDKSAIETKRDDAIAKSDLRNALVAAKVYYTDTETYADVTTEVLSDIEPSLTYASI
                     ENTAEDVVGFGDLSANKIVLVTKSAGGTYYCVAEDARYGTTFGESDSLAEVDTVAECH
                     NGWDGSSSSSGAAMPPTTVSAEEYPVDAAQVMRCSMEAGTVTTALDAYRARYGTEFAK
                     NSTELVEALIAADFLAAEPKYGTDENPPTTGTWSYDDTTGHITNMC"
     gene            complement(4556..>5537)
                     /locus_tag="VFX21_11755"
     CDS             complement(4556..>5537)
                     /locus_tag="VFX21_11755"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5096686.1"
                     /translation="RAMWEEGLRVAVRCMTEEPFTGHSGEFVTMPPRNVLPKPRQKPH
                     PPIWVACSRRDTIHLAAQKGIGALAFAFVDPEEAKYWVTDYHQTLADEGVPIGDTVNP
                     NIACVTTFMCHENEEVALARGLEGANFFGYSLAHYYVFGRHQPGVTNVWEEFEHRRAE
                     QGYDPTAVQAAAANQDRLGAKVVDQGVGGLRGAVGTPDQLREYLLRYEECGVDQVIFC
                     YQAGKNKHEHIMEALELFGKEVLPEFAERDEKLSRDKEQRLAPVIDKVMARKPAEDHP
                     PLPSADYAFPAIPRGMADRNGSDDFHKMLDEFEKNSAMGPSGIEGLINNR"
BASE COUNT          889 a         1907 c         1847 g          894 t
ORIGIN      
        1 ctggtacctc accaaacacg gcgtcggtgt gtggtcggcg acgccaccga ccagaccgtt
       61 cgcgctcgcc gccgacacgc aagcgcgcgt cgacgcgctc ccccactgcg aggccgccgg
      121 gctcgtcgag ggcgatgcga ccatcgaagc cacgtcggtt gcgttcgaac gcgacggcac
      181 accgagctcg ggcctggtcg cgctgcgtct cgacgagcgt cgccgcgccc tcgcccgctg
      241 caccgacccc gacctgctgc gcgcgatgac cgagtcgacc tgggaagggc gctcggtgac
      301 cgtccgcaac gacggatcga ccaacaccat cgccgggtag ggagaagacg tggccgatca
      361 actcgacgtc gacgcaatgc tcgaacgctt ccgccagcgg gccgcggcag tgaaggaccg
      421 gccgctgccg ccggtggcgg gcaacgagcg ccagatgttc atcgagcagg cgcagctcga
      481 cttccaagac ttcgcgatca tcggcgacgc gacctggacg ttcgagaacg gcgtgctgat
      541 cttgaaggtc gacctgtcgt aaggcccggg gatgttcggt cctccccgca cacgcctgga
      601 gccacaagag gctcagtatc tccgcatctg ctcgtcgggg cgctcgttga cgtccagacg
      661 ttgcaggaat gcccggtcga ccgcgaccca caccgtcttc gagaacggat agaggatgat
      721 cggcccgagc gccacgatcg ggatgcacac cgccagcagc tcgaccggtt cgacgtcggg
      781 aagttgcacg atcaacgtca cgacgaacgc gacggcgaag agcccgccgg tcatgatcat
      841 gttgaacgcg agggcaccgg tccagtagcc ctgctcgcgt tcgaagtgga gcccgcagcg
      901 cgggcagtcg ggcaccatca cgaaccaacg gcggaacagc ttgccgctgc cgcaacgcgc
      961 acaccgtttc gtgaagccac gccacaacgt cgtgaaccga ctcggttgct cagccacgat
     1021 gccgacgcta cccccggcgg gccaccagca ctcgatcccg gccagtgaga tcgggacgaa
     1081 tcgtgacgtc gtcgaaaccg gcggcccggg cgtacggcac catgtcggcg gcctgatgtg
     1141 gcgccagctc ggcaacgagg gcgccgcgcg gcgcgagcca cgcgggtgct tcgtcgacca
     1201 gatgggtcag ggcttcgagg cctgtcgggc cggcaacgag cgccgtgcgc ggttcgtagt
     1261 cgaggacgac aggcggcagc gcgatgactt cgtgctgcgc gacgtacggg gggttcgaca
     1321 cgacgagcgt caaccagcct cgcagctcac gaggcagcgc gtcgaaccac gagccgggtg
     1381 cgacgcgaac gcgcgtcgca ccgcaaccgg cgagattcgc gcgcgcgacg gcgagcgcgt
     1441 cctcactcgc gtcggtggcc cacacttcga cctcgggcag ttcgtgctcc agcgcgagcg
     1501 cgatcgcgcc cgatccggtg ccgaggtcgg cgacaacggc cgtcggtgcc gacgggaccg
     1561 cgccgcgcgc cttggcccgc cgcagcccga gccggaccgc ctcgtcgagc gcgacctcca
     1621 cgacgatctc ggtttccggg cgcgggatca gcacgcgcga gtcgaccatc aagtcgaggt
     1681 cgcgaaacgc ccaggagccg agtacgtact gcagcggctc acccgcggcg cgccgttcgc
     1741 acatcgcgtg cacgcgcgct tcggcccgcg cgatcgcgtc ggtgtcttcg atttcgagcc
     1801 actccccgcc cgcgtgtccc gagcactcct cggtgatgaa gcgcgcttcg gtgtcaggtg
     1861 actcgatgcc ggcgtcgcgt agccggtgcg cgacggcggc gcgcagtgtg cgccacttca
     1921 agagacctcg ccggcgagct ggcgggcgcg ttcgtcttca ccgagccggt cgatgatctc
     1981 gtcgagctcc cgttcgcccg cgagcacccg gtcgagagcg tgcagcgtga gacccggtat
     2041 gcggtggtcg gtgacgcggt tctccttgta gttgtacgtg cgaatctttt cggaccgccc
     2101 gcccgtcttc acctgaccct tgcgcgccgc gctcaactcg gccgcctgcc gttcctgctc
     2161 caactgcaac aagcgcgaac gcaggatgcg cagggccttg tccttgttct ggagctggct
     2221 cttctcgtct tggcacgaca cgacgagacc ggtcggcttg tgcgtcatgc gcaccgcgga
     2281 gtcggtcgtg ttcacggact gcccgcccgg tcccgacgag cggaacacgt cgatctcgag
     2341 gtcgttgggg tcgatctcga tgtcgacctc ctcggcttcc gggagcaccg cgacggtcgc
     2401 cgcgctcgtg tgcacgcgtc cttggctctc ggtcacgggc acgcgctgca cacgatgcgg
     2461 gccgccctcg tacttgaagc ggccccacgc ggcgtcgccc ttcaccgcga acgtgatgtc
     2521 gcgaaagccg ccgtgctccg acacctgctg gtcgagcact tcgagcttca ggccgttgcg
     2581 gtcggcgaag cgctggtaca tgcggaacag gtcgccggcc cagatgttcg cttcctcgcc
     2641 gccctcggtg ccctggatct cgacgatgac gttgcgccct tcgttggggt cgcggggcaa
     2701 cagcagctcc cggatctgct cggccagctc gtcgacgcgg gcctgcttct cggcgatctc
     2761 gcccttcagg aactcccgca tgtcggcatc cgactcgccc tcgagcagct cctgggcctc
     2821 ggcgacgtcg ttgccggccg cgcgccactc ttgataggcg cccacgagcg gcttcagctc
     2881 ggcgtggcgg cggcccgcgt cccgcgaggc ccgccggtcg ccggatgcgt agatccccgg
     2941 caactccgct tccagccgct cgagctcctg ctccaaatcc tcgagctgct cgaacatagg
     3001 cctgtcagct tatttagccc ccggggtccg attcggcact caaggcccca cgatcgaggg
     3061 tcgatcatcg gcgtagggac gggctggcgg ctcactgata tgccagggat ggcaggtcat
     3121 gaacgtcagc gacattgggc aaggccaggg aagcgttgcg acgcttctgc ggccgtacgc
     3181 aagcggtgga ttgccgctgg tgcacgctca ggctgacgcg cgggttgccg ccgaggtctc
     3241 cacgtccacg cacgaagtgc agcgttgggc gtggttcgac ctcaacggtg acggcacgat
     3301 cgacgacacc agcccgctgt acggcggtga cggcgtcctc gtttggaacg gctcgggggc
     3361 caagccctcg cacccgcgcc cggcagagca aagcaccagc acgcgttctt cagacgagca
     3421 ctcgacgcca gtgaaggtcg agcacgctcg ggccgcgtac acgcgttacg gccaggcatc
     3481 accgacgcac gaatccgcac cgcacgccgc cgtggcgtaa cgcgcgctcg tccgtccgga
     3541 tccaccccgg caccgccggg gatcttgtcg cgcgctcgcg tactgaccgt ctcgaagccg
     3601 tcaagcgcgc ctgcgcaacg tccgatgcaa caacgagctc gtggtgtggt cgcgggtgcc
     3661 tgcggtgggg gcggacgcga cagtggtgtt gcggccggcg atgcggcgga agcaccggcg
     3721 accacgccga gcgaagaaac gaaacgtggg gcagggcatg ctgggactcg aagaacgacc
     3781 gtccgcggat attccggtca tccggccggt gaagaggtcg agcggaccgg tcatcggtct
     3841 cgcgctcggg ctcgtcgtcg tgctcatcgc gggcgtgtgg ttcgtgacca accgcgacga
     3901 caagtccgcg atcgagacca agcgtgacga cgcgatcgcg aagtcggatc tccgcaacgc
     3961 attggtggcg gcgaaggtct actacaccga caccgagacc tacgccgacg tcacgacgga
     4021 ggtgctcagc gacatcgaac cgagcctcac gtacgcctcg atcgagaaca cggccgagga
     4081 tgtcgtgggc ttcggtgacc tgagtgcgaa caagatcgtc ctcgtcacca agagcgcggg
     4141 cgggacctac tactgcgtcg cggaagatgc gcgctacggc acgacgttcg gcgagagtga
     4201 ctcgctggcc gaggtcgaca ccgtcgccga gtgccacaac ggctgggacg ggagctcttc
     4261 gtcatctggt gcagcgatgc cgccgaccac ggtctcggcc gaggagtatc cggtcgacgc
     4321 cgcgcaagtg atgcgctgct caatggaagc cggcaccgtc accaccgcac tcgacgcgta
     4381 ccgcgcccgg tacggcacgg agttcgcgaa gaactcgacc gagctcgtcg aagcgctcat
     4441 cgccgcggac ttcctcgcag ccgagccgaa gtacgggacc gacgagaacc cgccgaccac
     4501 cggcacgtgg tcctacgacg acacgaccgg ccacatcacc aacatgtgct gaccgctacc
     4561 ggttgttgat gagtccctcg atgcccgagg gacccatcgc ggagttcttc tcgaactcgt
     4621 cgagcatctt gtggaagtcg tcggagccgt tgcggtcggc cataccgcgc gggatcgcgg
     4681 ggaacgcgta gtccgcgctc ggcagcggcg ggtggtcttc tgcgggtttg cgggccatga
     4741 ccttgtcgat gacgggcgcg agacgctgct ccttgtcgcg tgagagcttc tcgtcacgct
     4801 ccgcgaactc gggcagcact tccttcccga acaactcgag cgcttccatg atgtgctcgt
     4861 gcttgttctt gccggcctgg tagcagaaga tcacctggtc gacgccgcac tcctcgtacc
     4921 gtaacaagta ctcgcggagc tggtcgggcg tgccgactgc gccgcgcaat ccgcctacgc
     4981 cttggtcgac gaccttcgcg cccaaacggt cctggttcgc ggccgcggcc tgtaccgcgg
     5041 tggggtcgta gccctgttcg gctcggcggt gctcgaactc ctcccacacg ttcgtgacac
     5101 cgggttggtg tcgtccgaac acgtagtagt gcgcgagtga gtagccgaag aagttcgcac
     5161 cttcgagacc gcgtgcgagc gcgacctctt cgttctcgtg gcacatgaac gtcgtgacgc
     5221 atgcgatgtt cgggttcacc gtgtcgccga tcggcacgcc ctcgtctgcg agggtctggt
     5281 ggtagtcggt gacccagtac ttcgcctctt cggggtcgac gaacgcgaac gcgagcgcgc
     5341 cgatgccctt ctgcgcggcg aggtggatcg tgtcgcggcg gctgcacgcg acccagatcg
     5401 gcgggtgcgg cttctggcgc ggcttcggca acacgttgcg gggcggcatc gtgacgaact
     5461 cacccgagtg accggtgaac ggctcctcgg tcatgcaccg caccgcgacg cgtaggcctt
     5521 cttcccacat cgcgcgc
//
LOCUS       DASUGA010000595         6665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_665457, whole genome shotgun sequence.
ACCESSION   DASUGA010000595 DASUGA010000000
VERSION     DASUGA010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6665
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665457"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VFX21_11760"
     CDS             <1..286
                     /locus_tag="VFX21_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5096687.1"
                     /translation="DRFMWGSDYPHDEGTHPFTREHLRQLFSDTDPAELQQLLAQNAA
                     KLYEFDLNALKPLAQQYGPTVGEIHQPLTELPENPNEALLRAGAALRASE"
     gene            364..906
                     /locus_tag="VFX21_11765"
     CDS             364..906
                     /locus_tag="VFX21_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009472546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX5096688.1"
                     /translation="MRALVVYESMYGRTREIAHSVAQGLRSVYEVDTVHVDDATPELV
                     ENADLLVVGGPTHVHGMTSQRSRTAAVDAAANDSELTLDEHAPGEGLRQWLAALEGDA
                     IAEAAAFDTRIDASPMLTGRASRGIAKRLHRAGYRMIADPESFLVDSHSRLLPGEVNR
                     ARAWGESLCTTSHGAVPKPA"
     gene            complement(996..1877)
                     /locus_tag="VFX21_11770"
     CDS             complement(996..1877)
                     /locus_tag="VFX21_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5096689.1"
                     /translation="MTECIGLPAPPPVGAHALAADTFEDVCVFVTGAGTGLGKAIACE
                     FARVGASIVIASRNGDHLAAGQAAIEAVGAPVTTVQCDIRDADSIAAAFDAAEAAFGL
                     PGVLVNNAAANFPVPAEDMSPNAWRTVVDITLNGTYLCAREFGRRHLAAATPGSIINV
                     GASYAWTGGPGFAHSAAAKAGVKNLTETLAVEWGPYGIQVNGLVPGLFPHEDMTADIR
                     SNMDRTSEKDACQPALRVGERQELGWAATFLASPYARFISGHTLVVDGANWQRRSLTN
                     PPVVTIRDQMGKGSFEL"
     gene            complement(1880..2662)
                     /locus_tag="VFX21_11775"
     CDS             complement(1880..2662)
                     /locus_tag="VFX21_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020548588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5096690.1"
                     /translation="MSDSRVRLEIDGAIATITNDNPDKHNAFDDDMDLALFDALSELR
                     NHPEVRAIIWRGEGKSFSSGRDASSIGNLKVDLTHHELMRRGHRGIQQLWELDAPVIV
                     ACKGWVMGGSFQRALLCDIRVAAEGTRFRLPELTYGVIPDTGGVAVLHQMAGPGIVTD
                     MVLTGRVLGAEEALAHGVVSRVVPAEDLDATVREMAEQIASAPGVTVKLAREVVRHLS
                     VPQVRASMTDEMIYQTFISRSDDMAELRAARAEDRAPRYTGS"
     gene            complement(2686..4641)
                     /locus_tag="VFX21_11780"
     CDS             complement(2686..4641)
                     /locus_tag="VFX21_11780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX5096691.1"
                     /translation="MRLRYLAGIDGLRAISVLAVLVYHNYTVPIGRTEGPLPGGFLGV
                     EVFFVISGYLITSLLLDERRRTGGVAFGQFWLRRARRLLPALFLVIAFCITWSLLFMP
                     DAIESLKGDALGGVFYYSNWWQIAANHSYFAASSPELLKHLWSLAIEEQFYLVWPVVL
                     FFGLRRLGVKRMVLAMFGVAALSVIVMWSVAKPSPNWDPSRFVYYATVCRMSGLLLGA
                     LLAFLWTPRRVRGRTGPGARWVLNLVGAVGLTGLFWSFAHFGFQSPGLYTRGGFVLVD
                     ILTVLVLAAIVHPKADWNWVLGNRPLVWIGLRSYGIYLWHYPLFWILTKRNIEDWIGF
                     APPNWLFYGLRFGVTIGIAELSFRFVEMPIRHGAIANFIDRFRASRGRYRRRLAVVGT
                     MVVTMFAFGTAVLASGLANASPEQTSDDNIANHAAEEQLDPEAARADALAAARAAAAS
                     TTAVPATDTTTTVPAVPVDPNATTVPTAAPTTVAPPVTPTGVFAIGDSVMLGAKNALQ
                     QKIPGIIVDAVVSRQFAHAISVVQFYKDNGLLPPVVVVHLGTNGRFGDGEFDTMMATI
                     GPERQAYFLTAREPRSWEAEVNATLTRGVQRHPNAHLIDWRQFSGCHSDWFARDGFHV
                     NGVGAAGYAEFVNAHITNQSASLQYCP"
     gene            complement(4753..5931)
                     /locus_tag="VFX21_11785"
     CDS             complement(4753..5931)
                     /locus_tag="VFX21_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5096692.1"
                     /translation="MNAGIDVARIRAATPGCERVAHLNNAGAALAPQSVLDTVVAYLI
                     READLGGYEAAEEATDRIEHVYGCVATMLGAHGDEIALVESATRAWTTYFSARRFEPG
                     QRILTHRVEYASNFIPMLQAVRRDGVAIDVVPSTPTGEIDVDALREMIDDRVALVALT
                     HVPSQGGLVNPAAAVGAVTRAAGVPFVLDACQSAGQLPLDVEAIGCDALVATGRKFLR
                     APRGTGFLYVRRAHMHGLEPEAPDLRGAEWTGAATYEVRDDARRFEQWERSVANVLGL
                     GAAVDLVLELGIGAIAARVMALGAELRAALASVDGVVLRDEGREQCGIVTFTLEGHDV
                     DDVKRALRAKHVNVWTIDATTAQLDFGARDLPAVVRASVHYYNTSEELEKATTAVRSV
                     "
     gene            complement(5928..>6665)
                     /locus_tag="VFX21_11790"
     CDS             complement(5928..>6665)
                     /locus_tag="VFX21_11790"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5096693.1"
                     /translation="HRTFFDRAPAWQELQSLDLDGRLAALDDDTRREELLREAEANTA
                     PMPWNGVYVLTDDAVDYSADPSTSLARHAEKAGETIAQAFVRISRETRGRALFNFPFL
                     NQRMDAVEEMLDNPRVMIGLGDSGAHVGLIMDASLPTWFLLHWVRDREKYPIEDAIRR
                     MTSETAGLFGVTDRGVLTPGAYADVNVLDLDQLALPLPEYVHDFPAGAGRYLQRSKGY
                     RTTIVNGGVFMEGDQPTGVHAGVTLRA"
BASE COUNT         1057 a         2257 c         2378 g          973 t
ORIGIN      
        1 cgaccgcttc atgtggggga gtgactaccc gcacgacgag ggcacgcacc cgttcacacg
       61 ggagcacctg cggcagttgt tctccgacac cgatcccgcg gagttgcagc aactcctcgc
      121 gcagaacgcc gccaagctct acgagttcga cctcaacgcg ctgaagccgc tcgcgcagca
      181 gtacggcccg acggtcggcg agatccacca gccgctgacg gagctcccgg agaacccgaa
      241 cgaagcctta ctgcgagcag gcgccgcact ccgcgcgagt gagtgacgcg ggacctttgc
      301 ctctatccaa ccggcggttc cgggaggacg atgtcttcgg gacatccgac cggaggtgcg
      361 gagatgcgag cactggtcgt ctacgagtcg atgtacggac ggacccgtga gatcgcgcac
      421 tcggtcgcgc aagggctgcg gtccgtgtac gaggtcgaca ccgtccatgt cgacgacgcc
      481 acgccggagc tggtcgagaa cgccgacctg ctcgtcgtcg ggggcccgac gcacgtgcac
      541 ggcatgacgt cgcagcgttc ccgcaccgcc gcggtcgacg ccgcggcgaa cgacagcgag
      601 ctgacgctcg acgagcacgc gcccggtgag ggattgcggc agtggctggc cgcactcgag
      661 ggcgacgcga tcgcggaagc ggccgcgttc gacacccgca tcgacgcgtc accgatgctg
      721 accggccggg cgagccgcgg cattgcgaag cggctgcacc gtgccggcta ccgcatgatt
      781 gccgaccccg agagcttcct cgtcgactcg cacagccgac tgctcccggg cgaagtgaac
      841 cgcgcccgcg cgtggggcga atctctgtgc accacgtcgc acggcgccgt gccgaagcct
      901 gcgtaggcac tggccggagc ggagggcggg ccaacaatcc gagccacctc acgcgagcac
      961 gaccggagcg accaaagaga gatcgggcgc attgctcaca actcgaagct ccctttcccc
     1021 atctggtctc tgatcgtgac gaccggcggg ttggtcaggg agcggcgttg ccagttggcg
     1081 ccgtcgacga cgagggtgtg gcccgagatg aagcgcgcgt agggcgacgc gaggaaggtc
     1141 gcggcccagc cgagctcttg ccgttcgccg acgcgcagcg cgggctggca ggcgtccttc
     1201 tcgctcgtgc ggtccatgtt cgagcggatg tcggcggtca tgtcctcgtg cgggaacagg
     1261 cccggtacga gcccgttcac ctggatgccg tacggacccc actcgacggc gagcgtctcg
     1321 gtcaggttct tcacgccggc cttcgccgcg gccgagtggg cgaacccggg cccgcccgtc
     1381 cacgcgtacg acgcgccgac gttgatgatc gaacccggcg tcgccgccgc gaggtggcga
     1441 cggccgaact cgcgggcgca gagatacgtg ccgttcaacg tgatgtcgac gaccgtccgc
     1501 cacgcgttcg gcgacatgtc ctcggccggc accgggaagt tggcggccgc gttgttgacg
     1561 agcacgcccg gcaatccgaa cgcggcctcg gctgcgtcga aggcggcggc gatcgagtcg
     1621 gcgtcgcgga tgtcgcactg caccgtcgtt acgggggcgc cgacggcttc gatcgcggcc
     1681 tggccggcgg cgagatggtc gccattgcgg ctcgcgatga cgatcgacgc accgacccgt
     1741 gcgaactcgc acgcgatcgc cttgcccaat cccgtccccg caccggtcac gaagacacac
     1801 acgtcctcga acgtgtcggc ggcgagcgcg tgcgcgccga ccggcggcgg tgcaggaagt
     1861 ccgatgcatt cggtcacgat cagctcccgg tgtagcgagg tgcgcgatct tcggcgcgtg
     1921 cggcgcgcag ttcggccatg tcgtcactgc ggctgatgaa ggtttggtag atcatctcgt
     1981 ccgtcatcga tgcgcgcact tgcggcaccg acaggtgacg gacgacttcg cgcgccagct
     2041 tcaccgtcac gcccggtgcg ctcgcgatct gctcggccat ctcgcgcacg gtcgcgtcga
     2101 gatcctcggc gggcacgaca cggctcacga cgccgtgggc gagcgcttcc tcggcgccga
     2161 gcacgcgtcc ggtgagcacc atgtcggtga cgatgccggg cccggccatc tggtgcagca
     2221 ccgcgacgcc gccggtgtcg gggatgacgc cgtaggtcag ctcggggagg cggaagcggg
     2281 tgccctcggc ggcgacgcgg atgtcgcaca acaacgcccg ctggaacgag ccgcccatga
     2341 cccagccctt gcacgcaacg atcaccggcg cgtcgagctc ccacagctgc tggatgccgc
     2401 ggtggccgcg gcgcatcagc tcgtggtgcg tgaggtcgac cttcaggttc ccgatcgacg
     2461 acgcgtctcg gcccgacgag aacgacttcc cctcgccgcg ccagatgatc gcccgcacct
     2521 cgggatgatt gcggagctcg gacagcgcgt cgaacagggc gaggtccatg tcgtcgtcga
     2581 acgcgttgtg cttgtcgggg ttgtcgttcg tgatcgtggc gatggcgccg tcgatctcga
     2641 gccgcacgcg cgagtcgctc atgcccgcgg aagctactcg actctctacg ggcagtattg
     2701 aagtgacgcc gactggttcg tgatgtgggc gttcacgaac tcggcatagc cggccgcgcc
     2761 gacgccgttg acgtggaagc cgtcccgtgc aaaccagtcg ctgtggcagc cggagaactg
     2821 gcgccagtcg atcaggtgcg cgttcgggtg ccgttgcacg ccgcgagtga gcgtcgcgtt
     2881 gacctcggcc tcccacgatc gcggctcgcg cgcggtgagg aagtacgcct gccgctcggg
     2941 cccgatggtc gccatcatcg tgtcgaactc gccatcgccg aagcggccgt tggtgccgag
     3001 gtggacgacg acgaccggcg ggagcagccc attgtccttg tagaactgca cgaccgagat
     3061 cgcgtgcgcg aactgccggc tcacgaccgc gtcgacgatg atgccgggga tcttctgctg
     3121 aagcgcgttc ttcgcgccga gcatcaccga gtcgccgatg gcgaacacgc cggtcggggt
     3181 gaccggcggt gcgacggtcg tgggcgcggc ggtgggcacc gtcgtcgcat tgggatcgac
     3241 gggcaccgcg ggaacggtcg tcgtcgtgtc ggtcgccggg accgccgtcg tcgacgcggc
     3301 agcggcgcgc gccgccgcga gcgcatccgc acgcgcggcc tccggatcga gttgctcctc
     3361 ggccgcgtgg ttggcgatgt tgtcgtcgct ggtctgctcg ggcgacgcgt tggcgagccc
     3421 gctggccagg acggcggtgc cgaacgcaaa catggtgacc accatcgttc cgacgacggc
     3481 gagccgccgg cggtaccgcc cgcgcgatgc acggaaccgg tcgatgaagt tggcgatcgc
     3541 gccgtggcgg atcggcatct ccacgaaccg gaacgacagc tcggcgatcc cgatcgtcac
     3601 cccgaagcgg aggccgtaga agagccagtt cggcggtgcg aagccgatcc agtcctcgat
     3661 gttgcgcttg gtcaggatcc agaacagcgg gtagtgccag aggtagatgc cgtaggaccg
     3721 caggccgatc cacaccagcg gccggttgcc gagcacccag ttccagtcgg ccttcgggtg
     3781 cacgatggcg gcgagcacga gcacggtgag gatgtcgacg aggacgaacc cgccgcgcgt
     3841 gtacaggccc gggctctgga acccgaagtg cgcgaacgac cagaagagtc cggtcaggcc
     3901 gaccgcgccg acgaggttca ggacccagcg cgcgcccgga ccggtccggc cgcgcacgcg
     3961 ccgcggggtc cacaggaatg cgagcaacgc gccgagcagc aggcccgaca tgcggcacac
     4021 ggtcgcgtag tagacgaagc gcgacgggtc ccagttcggg cttggtttcg ccaccgacca
     4081 catcacgatg acggagagcg cggccacgcc gaacatggcg agcaccatgc gcttcacgcc
     4141 gaggcgcctg aggccgaaga agagcacgac cggccacacg aggtagaact gctcttcgat
     4201 cgcgagcgac cagaggtgct tcaggagctc gggactgctg gccgcgaagt acgagtggtt
     4261 ggccgcgatc tgccaccagt tgctgtagta gaagacgccg ccgagtgcgt cacccttcag
     4321 cgactcgatc gcgtcgggca tgaacagcag tgaccaagtg atgcagaacg cgatgacgag
     4381 gaacagcgcg ggaagcaggc ggcgggcgcg gcgcagccag aactgtccga acgcgacgcc
     4441 gccggtgcgc cgccgttcgt cgaggaggag cgacgtgatc aggtaaccgc tgatcacgaa
     4501 gaagacttcg acgccgagga aaccgccggg caacgggccc tcggtccgcc cgatcggcac
     4561 cgtgtagttg tggtagacga gcaccgcgag gaccgagatc gcgcgcaacc cgtcgatgcc
     4621 cgcgaggtaa cgcagtcgca ccgggcgatg gtcgatcggg gaagcgggtg cgtcctgcat
     4681 cactgagaac ttcggcgcgg cgggggcagc gggctccgga ggagtctacc gacggtggcg
     4741 cgcgaaccgt ggtcagacgg atcgcaccgc ggtggtcgcc ttttccagct cttcggacgt
     4801 gttgtagtag tgcaccgagg cgcgcacgac cgcgggcaga tcgcgagcgc cgaagtcgag
     4861 ctgggccgtt gtcgcgtcga tcgtccacac gttgacgtgc tttgcgcgca gcgcccgctt
     4921 gacgtcgtcg acgtcgtgac cctccagggt gaacgtcacg atgccgcact gctcacgacc
     4981 ttcgtcgcgc agcaccacgc cgtcgaccga cgcgagcgcg gcgcgcagtt cggcgccgag
     5041 ggccatgacg cgtgcggcga tggcgccgat gccgagctcg aggacgaggt cgaccgccgc
     5101 gcccagcccg agcacgttcg cgacggaccg ttcccattgc tcgaatcggc gcgcgtcgtc
     5161 gcgcacctcg taggtcgcgg cgccggtcca ttcggcgccg cgcagatcgg gcgcctccgg
     5221 ctcgagcccg tgcatgtgcg cgcgccgcac gtacaggaag ccggtgccgc gcggtgcgcg
     5281 caggaacttg cggccggtgg cgaccagcgc gtcacagccg atcgcctcga cgtcgagcgg
     5341 gagctgaccc gcggactggc acgcgtcgag cacgaacggc acaccggcgg cgcgggtgac
     5401 ggcgccgacc gcggccgccg ggttcaccaa accgccctga ctgggcacat gggtcagcgc
     5461 cacgagcgcg acccggtcgt cgatcatctc ccggagggcg tcgacgtcga tctcaccggt
     5521 aggcgtgctc gggacgacgt ctatcgcgac gccgtcgcgc cgcaccgctt gcagcatcgg
     5581 gatgaagttg ctcgcgtact cgacgcggtg ggtgaggatc cgctgccccg gctcgaagcg
     5641 gcgcgcagag aagtaggtcg tccacgcgcg tgttgcgctc tcgacgagcg cgatctcgtc
     5701 gccgtgcgcg ccgagcatcg tcgccacaca gccgtagacg tgctcgatgc gatcggtcgc
     5761 ttcttccgcg gcctcgtagc cgccgaggtc ggcctcgcgg atcagatagg cgaccacggt
     5821 gtcgagcacc gattgcggcg caagggcggc gcccgcgttg ttcaggtgcg cgacgcgctc
     5881 gcagcccggc gtggcggccc ggatccgtgc aacgtcgatg ccggcgttca agcccgcagc
     5941 gtgacgccgg cgtgcacgcc ggtcggctgg tcgccctcca tgaacacgcc gccgttgacg
     6001 atcgtggtgc ggtagccctt cgaccgttgc aggtagcggc cggccccggc cgggaagtcg
     6061 tggacgtact cgggcagcgg caacgcgagc tggtcgaggt cgagcacgtt cacgtccgcg
     6121 tacgcgcccg gcgtgagcac gccgcggtcg gtgacgccga agaggcccgc ggtctccgac
     6181 gtcatccggc ggatggcatc ctcgatcggg tacttctcgc ggtcgcgcac ccagtgcagc
     6241 aggaaccacg tcggcagcga cgcgtccatg atcaggccga cgtgcgcgcc ggagtcgccg
     6301 aggccgatca tcacgcgcgg gttgtcgagc atctcctcga ccgcgtccat gcgctggttc
     6361 aggaacggga agttgaacag cgcgcggccg cgcgtctcgc gggagatgcg gacgaacgcc
     6421 tgcgcgatcg tctcgcccgc cttctccgcg tgccgcgcca gtgaggtgct cgggtccgcg
     6481 gagtagtcga ccgcgtcgtc ggtgagcacg tacacgccgt tccacggcat cggcgcggtg
     6541 ttggcctcgg cttcgcgcaa cagctcctcg cggcgcgtgt cgtcgtcgag cgcggcgaga
     6601 cggccgtcga ggtcgagcga ctgcagttcc tgccacgcgg gcgcgcggtc gaagaacgtg
     6661 cggtg
//
LOCUS       DASUGA010000596         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_196901, whole genome shotgun sequence.
ACCESSION   DASUGA010000596 DASUGA010000000
VERSION     DASUGA010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196901"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VFX21_11795"
     CDS             complement(<1..679)
                     /locus_tag="VFX21_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX5096694.1"
                     /translation="MRRTKIVATVGPATDSRPAMHALLAAGADVVRLNAAHGDVPTHT
                     ERARQARSIATELGRVVGVLVDLPGPKMRSGPIAGDEVELDAGAQFTLTAAAIDGDTT
                     QVSTTLPELARWVDPGDEVYLADGAIVLKVERIDGDDVVCEVMRGGILRSRKGMHVPR
                     AEAHVDPFTDADAIALKMAVSIKADFVGLSFIRRPEDCEAVRARLPKRGHRPALVAKI
                     ETAAALDH"
     gene            713..1063
                     /gene="folB"
                     /locus_tag="VFX21_11800"
     CDS             713..1063
                     /gene="folB"
                     /locus_tag="VFX21_11800"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:NF014233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX5096695.1"
                     /translation="MDRIKISGLRELGVHGVLPEEQIRPQPFEVDLEMTVDLVPAGAS
                     DELDDTVDYSAVCEAVSRVVASEQYRLLERLAARIAEVCLADPRVKNVSVEVRKLHPP
                     VRAMLDYVAVRVER"
     gene            1060..1560
                     /gene="folK"
                     /locus_tag="VFX21_11805"
     CDS             1060..1560
                     /gene="folK"
                     /locus_tag="VFX21_11805"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017618539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX5096696.1"
                     /translation="MTQAFLALGSNLGDRAAHLQAAVDGLARAPGTRVVAVSRVYETA
                     PVGGPEQGAYLNAVVEVDTDLDPHALLAVGQQLERAAQRVRNERWGPRTLDVDVLLYD
                     DLEIDSEDLVVPHPRMWERAFVLVPLRDIAPELVREPLDPADEGVWPAAIALTLPWTN
                     DSAPSP"
     gene            1530..>2187
                     /locus_tag="VFX21_11810"
     CDS             1530..>2187
                     /locus_tag="VFX21_11810"
                     /inference="COORDINATES: protein motif:HMM:NF022187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEX5096697.1"
                     /translation="MDERQRTVALIGPGRAGTTIALGLLEQGWTVTAVAGRAPDAAST
                     ASAAAILASRTTLVSEVGTGASVVIVATPDRAIEQAAETAEPTIEPGALVFHLAGSRG
                     LDAFEGLLARRTGVRVGALHPLQSFPSTTVGLERLPGTWAAVAGDPQIAEIARTLGMR
                     PFDLPDSERTRYHTAAVVASNHLVALLGQVERLAASAGVPFEAFGPLVIGSVQNAFQM
                     G"
BASE COUNT          317 a          783 c          740 g          347 t
ORIGIN      
        1 ggtgatcgag cgccgcggcc gtttcgatct tggcgacgag cgcgggccgg tgaccacgct
       61 tgggcagccg ggcgcgcacg gcctcgcagt cctcggggcg ccggatgaac gacaacccga
      121 cgaagtcggc cttgatcgac accgccatct tcagcgcaat cgcgtcggcg tcggtgaacg
      181 gatcgacgtg cgcttcggcg cgcggcacgt gcattccctt gcgggatcgc aagatgccgc
      241 cgcgcatgac ttcacacacg acgtcgtcgc cgtcgatgcg ctcgaccttc aacacgatcg
      301 caccgtcggc gaggtacacc tcgtcgcccg gatcgaccca gcgcgcgagt tcgggcagcg
      361 ttgtcgagac ctgcgtcgtg tcgccgtcga tcgccgccgc ggtcagagtg aactgcgcgc
      421 cggcgtcgag ttcgacctcg tcgcccgcga tcggtccgct gcgcatcttc gggcccggca
      481 ggtcgacgag cacaccgaca acgcggccca actccgttgc gatcgagcgc gcttggcgcg
      541 cccgttcggt gtgggtcggc acatcgccgt gggcggcgtt caaccgcacg acgtcggcgc
      601 cggccgcgag gagcgcgtgc atcgccggcc gggagtcggt cgcgggcccc acggtggcga
      661 cgatcttcgt gcggcgcatt gctccggaat agcacgccag tagtgtcgct ccgtggaccg
      721 catcaagatc tcgggcttgc gggagctcgg cgtgcacggc gtgctcccgg aagagcaaat
      781 ccgaccgcag ccgttcgagg tcgatctcga gatgacggtc gatctcgtac ccgcgggcgc
      841 gagcgacgaa ctcgacgaca ccgtcgacta ctcggccgtg tgcgaagcgg tcagccgcgt
      901 ggtcgcatcc gagcagtacc gcctcctcga acggctcgcg gcgcgtattg cggaggtgtg
      961 tctcgccgac ccgcgcgtga agaacgtcag tgtcgaggtg cgcaagctgc atccgccggt
     1021 acgcgcgatg ctcgactacg tcgcagttcg agtcgagcgt tgacgcaggc gttcctcgcg
     1081 ctcggttcca acctcggtga ccgcgcggcg cacctgcagg ccgcggtcga cggtctggcg
     1141 cgtgcgcccg gcacccgcgt cgttgccgtt tcacgcgtgt acgaaacggc tccggtcggc
     1201 gggcccgagc aaggtgcgta cctcaatgcc gtcgtcgaag tcgacacgga cctcgacccg
     1261 cacgcgttgc ttgccgtcgg ccagcaactc gagcgagccg cgcagcgggt gagaaacgag
     1321 cgctggggcc cgcgaacact cgacgtcgac gtgctgctct acgacgacct cgagatcgat
     1381 tccgaagatc tcgtcgtgcc gcatccgagg atgtgggagc gagccttcgt cctcgtgccc
     1441 ttgcgcgaca tcgcgcccga actcgtacgc gaaccactcg atcccgccga tgaaggtgtg
     1501 tggcccgcgg cgatagcctt gacccttcca tggacgaacg acagcgcacc gtcgccctga
     1561 tcgggcccgg gcgcgcgggc acaacgatcg cgctcggctt gctcgaacag ggctggacgg
     1621 tgacggccgt tgccggccgt gctcccgacg ccgcgtcgac tgcgtcggcg gccgcgattc
     1681 tcgcgtcccg caccacactc gtttccgagg tcggaaccgg cgcgtccgtc gtcatcgtcg
     1741 cgacgcccga ccgtgccatc gaacaagccg ccgagaccgc ggagcccacg atcgaacccg
     1801 gcgcgctcgt cttccacctc gccggctcgc gcggactcga cgcgttcgaa ggccttctcg
     1861 cccgacgcac gggtgtgcgc gtcggcgcgc tgcatccgct gcaatcgttc ccgtcgacaa
     1921 cggtgggtct cgaacgcctc cccggcacgt gggccgcggt cgccggtgat ccgcagattg
     1981 cggagatcgc ccgcacgctc ggcatgcgcc cgttcgacct ccccgactcc gagcgcaccc
     2041 gctaccacac ggccgcggtc gtggcgtcga accacctcgt cgcgctgctc ggccaggtgg
     2101 agcgactcgc ggcgagcgcg ggcgtgccgt tcgaagcgtt cggtccgctg gtcatcggct
     2161 cggtgcagaa cgctttccag atgggcc
//
LOCUS       DASUGA010000597         4308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1863088, whole genome shotgun sequence.
ACCESSION   DASUGA010000597 DASUGA010000000
VERSION     DASUGA010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1863088"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VFX21_11815"
     CDS             <1..208
                     /locus_tag="VFX21_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007636232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096698.1"
                     /translation="VHAIVVLHDGQVMTDEDVVAHARELIAGFKLPKSIEFRTEPLPL
                     SGAMKVLKKDLRAPYWEGQDRGVS"
     gene            complement(474..2162)
                     /locus_tag="VFX21_11820"
     CDS             complement(474..2162)
                     /locus_tag="VFX21_11820"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX5096699.1"
                     /translation="MPDCNAVIVGSGPAGATAAEVLTAAGWSVVVLEKGVNHLLSLDA
                     PFGPLGHVSNDEIKFGRRFFLGPDPMIEPRTLRRDDADGDRQFTGDVNNLPSTVGGGG
                     FHADGKLPRFRAVDFKARSELGPIDGADVVDWPIDYDEMEPYYAQAEKVVGVAGLAGA
                     NPFAEWRSGPYPMPPGADMFGAVLSTEAAARLGYHPYPAPTGVNSVEYDGRPACNNCG
                     FCGYYGCPIEAKGDPVAPLRHALETGRCDIRPESYVERIVLDASGRRARGVQYIDRDG
                     NRQVVTADYVVVAGGAFETPRLLLRSEVGNSSGLVGRHLMFHFQTFVVASFPFRLHGH
                     RGRSVTHLHDDHMLVDDDVRAFAKEHGLPYFRAGIVEHGGSGGPVQEATYTPAGRKHA
                     PTMLDSPMRDRLWVFTVQGEDLPQVTNRIDLDPTVRDVHGHPAGRVTYDVHRHEVVAS
                     RYYAPKLEAIMMAAGAEHAFTAHSPALDGDRTAAGAPSASRHIMGTCRMGDDPRTSVV
                     DPWQRFHDVENMICTDSSVFPTSTGYGPTLTIVALAIRACRALAGLEPLRSTKP"
     gene            complement(2155..2724)
                     /locus_tag="VFX21_11825"
     CDS             complement(2155..2724)
                     /locus_tag="VFX21_11825"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEX5096700.1"
                     /translation="MTLRFLTQHEYDVIACAADVLMPPEGDHPGGCALGVADYVDTLL
                     GAFTFDPPRIFAGGPFSGRAGGDASFGEFMPLSRLEELAWRTRIEGSRGIAQREFNGP
                     VVGWQDTYRAAIAALGDDFCAIDVREQSRRLHAQPEFRALFYEHACEGAYGAPEYGGN
                     RDLAGWRAIEFPGDSQPRGYTDDEVRGRA"
     gene            2775..3743
                     /locus_tag="VFX21_11830"
     CDS             2775..3743
                     /locus_tag="VFX21_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007384363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5096701.1"
                     /translation="MGLGYMESPAIEVKGLAKHYGDVVALDGIDFTVPRGTILGLLGP
                     NGAGKTTAVRILTTILTPDRGSARVLGIEVGENPQAVRERIGLAGQFAAVDENLTGRE
                     NLRLVGRLTHMPKPVIAARATELLARFDLTDAADRVVRTYSGGMRRRLDLAAALVHRP
                     PILFLDEPTTGLDPRSRIDLWGVIEELVRDGTTLLLTTQYLEEADRLADNIIVIDHGH
                     VIAEGTSAELKAQLGATIVEVGFEDGASVQRAQSELCSVGLCDARVELNLVEVRVNEG
                     AKSVLEVVRVLDQAQLVPSTLNVREPTLDDVFLSLTGHAAEEESAA"
     gene            3740..>4308
                     /locus_tag="VFX21_11835"
     CDS             3740..>4308
                     /locus_tag="VFX21_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004921166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5096702.1"
                     /translation="MTATTFDAVPITTRHKSHAISDAITIAWRTLLGMTRTPQVLVFS
                     TIQPILLVLMFRYVFGGAIQVPGVEYVNYLMAGVFAQVVVFGAVQTGIGLAEDLQKGL
                     IERFRSLPMARSAVLAGRTLADLVRNVFVVAVMVAVGFIVGWRVHTGVPGFLAGIGVL
                     LLFGFAMTWVFAIVGMMATNAEAAQALAFP"
BASE COUNT          676 a         1432 c         1532 g          668 t
ORIGIN      
        1 ggtgcacgca atcgtcgtgt tgcacgacgg ccaggtcatg accgacgaag acgtcgtcgc
       61 gcacgcccgc gagctgatcg cgggtttcaa gctgccgaag tcgatcgagt tccggaccga
      121 accgctcccg ctgtccggcg cgatgaaggt cctgaagaaa gacctccgcg cgccgtactg
      181 ggaaggccag gaccgcggcg tcagctgaag gcgctgttcg ggcgtcttac aggggcggaa
      241 ttcgaccctg taagacgccc gacagtcgtg aggcgatgcg acgaggcttc gatgttcggt
      301 ccttccgcgg cgcgtagcgc ccctcgagga tcggcgcgcg aagtctcacg aggcggtggc
      361 cgcggtgtcc gctcggcgcg aagcgccctc gagaccttct cggccactcg cggagtcgga
      421 gggcgtgcca acacgcgcgc tccaacacgc gaacacgccg gagcgacaaa ggcttatggc
      481 ttcgtgctcc tgagtggttc gaggccggcg agggcgcggc aggcgcggat cgcgagggcg
      541 acgatcgtca gcgtcgggcc gtaaccggtc gacgtcggga acaccgacga gtcggtgcag
      601 atcatgttct cgacgtcgtg gaaccgttgc caggggtcga cgacactcgt gcggggatcg
      661 tcgcccatgc ggcaggtgcc catgatgtgg cgcgatgccg acggcgcgcc cgccgcagtg
      721 cggtcgccgt cgagcgcggg tgagtgcgcg gtgaacgcgt gctcggcgcc cgcggccatc
      781 atgatcgctt ccagcttggg cgcgtagtaa cgcgacgcga cgacctcgtg gcggtggacg
      841 tcgtacgtca cgcggcccgc ggggtgaccg tgcacgtcgc gcactgtcgg gtcgaggtcg
      901 atgcggttcg tgacctgcgg caggtcctcg ccctgcaccg tgaacaccca gaggcggtcg
      961 cgcatcggcg agtcgagcat cgtcggcgcg tgcttacgac ccgccggcgt gtacgtcgct
     1021 tcctggaccg gtccaccgct cccgccgtgc tcgacgatgc cggcgcggaa gtaggggaga
     1081 ccgtgctcct tggcgaacgc gcgcacgtcg tcgtcgacga gcatgtggtc gtcgtgcaga
     1141 tgcgtcaccg accgcccgcg gtggccgtgc aggcggaacg ggaacgacgc cacgacgaac
     1201 gtctggaagt ggaacatgag gtggcgaccc accagcccgg acgagttgcc gacctcggag
     1261 cgcaacagca agcgcggcgt ctcgaatgcg ccgcccgcga cgacgacgta gtcggccgtc
     1321 acaacttgtc ggttcccgtc tcgatcgatg tactgcacgc cgcgcgcccg ccggccgctc
     1381 gcgtccagca cgatgcgctc gacgtagctc tcgggtcgga tgtcgcagcg gccggtctcc
     1441 agcgcgtggc gcaagggcgc gacgggatcg cccttcgctt cgatcgggca gccgtagtag
     1501 ccgcagaacc cgcagttgtt gcacgccggc cggccgtcgt actcgacgga gttgacgccg
     1561 gtcggcgcgg gatacgggtg gtagccgaga cgcgccgcgg cttcggtgct gagcaccgcg
     1621 ccgaacatgt cggcgccggg cggcatggga tacggacccg aacgccattc cgcgaacggg
     1681 ttcgcgcccg cgagccccgc gacgccgacg accttctcgg cctgcgcgta gtacggctcc
     1741 atctcgtcgt agtcgatcgg ccagtcgacg acgtcggcac cgtcgatcgg acccaactcc
     1801 gaacgcgcct tgaagtcgac ggcgcgaaag cgcggcagct tgccgtcggc gtggaagcca
     1861 ccgccgccga ccgtggaggg aaggttgttc acgtcacccg tgaactggcg gtcgccgtcc
     1921 gcgtcgtcgc ggcgcagcgt gcgcggttcg atcatcgggt cgggaccgag gaagaaccgg
     1981 cggccgaact tgatctcgtc gttggacacg tggccgagcg gcccgaacgg cgcgtcgagt
     2041 gagagcagat ggttgacgcc cttctcgagc acgacgaccg accagcccgc ggcggtgagc
     2101 acttcggccg cagtggcgcc cgcgggaccg gaaccgacga tgacggcatt gcaatcaggc
     2161 acgaccgcgc acctcgtcgt cggtgtaccc gcgtggctgc gagtcgccgg ggaactcgat
     2221 cgcgcgccag ccggcgagat cacggttgcc gccgtactcc ggcgcgccgt atgcgccttc
     2281 gcacgcgtgc tcgtagaaca gcgcgcggaa ctcgggttgc gcgtgcaatc gccgggactg
     2341 ctcgcggacg tcgatcgcac agaagtcgtc gccgagcgcg gcgatcgcgg cgcgatatgt
     2401 gtcttgccag ccgacgacgg gaccgttgaa ctcgcgttgc gcgattccac gggagccctc
     2461 gatgcgcgtc cgccacgcga gctcctcgag ccgcgagagc ggcatgaact cgccgaacga
     2521 tgcgtcgcca ccggcgcggc cgctgaaggg accaccggca aagatgcgcg gcgggtcgaa
     2581 ggtgaacgcg ccgagcaggg tatcgacgta gtcggcgacg ccgagggcac acccaccggg
     2641 gtgatcaccc tcgggtggca tcagcacgtc ggccgcacac gcgatgacgt cgtactcgtg
     2701 ttgcgtgagg aagcgcagcg tcattggacc gggagcgtac gcgaacaggg ttcgaggcat
     2761 atggttcgag gcatatgggt ctgggttaca tggagtctcc ggcaatcgaa gtaaaagggc
     2821 tcgccaagca ttacggcgac gtcgtcgcgc tcgacggcat cgacttcacg gtgccgcgcg
     2881 gcacgatcct cggcttgctc ggcccgaacg gcgcgggaaa gacaacggcc gttcgcatac
     2941 tcacgacgat tctcacgccc gaccgcggga gcgcgcgggt gctcggtatc gaggttggcg
     3001 agaatccgca ggcggtgcga gagcgcatcg gcctcgcggg ccagttcgcg gccgtcgacg
     3061 agaacctgac cgggcgcgag aacctccgcc tcgtcgggcg gctcacgcac atgcccaagc
     3121 cggtcatcgc ggcacgcgcc accgagttgc tggcgcgctt cgacctcacc gacgcggccg
     3181 accgtgtggt gcgcacgtat tcgggcggca tgcggcggcg actcgacctc gcggccgcgc
     3241 tcgtgcaccg cccgccgatc ctgttcctcg acgagccgac gaccggtctc gacccgcgca
     3301 gccgcatcga cctgtggggc gtcatcgagg agctcgtacg cgacggcacg accttgctgt
     3361 tgacgacgca gtacctggag gaagccgacc gcctcgcgga caacatcatc gtgatcgacc
     3421 acggtcacgt gatcgccgag ggcacctcgg ccgagttgaa ggcgcagctc ggcgccacga
     3481 tcgtcgaggt cggcttcgaa gacggcgcct cggtacagcg cgcacagtcc gagttgtgca
     3541 gcgtcgggct gtgcgacgcc cgggtcgagc tcaatctcgt cgaggtgcgc gtcaacgagg
     3601 gcgcgaaatc ggtgctcgag gtcgtgcggg tgctcgacca ggcgcagctc gtgccgtcga
     3661 cgctcaacgt gcgggaaccc acgctcgacg acgtgttcct gtcgctgacc gggcacgcgg
     3721 ccgaggagga gtcggcagca tgaccgcaac caccttcgac gcggtgccga tcacgacccg
     3781 ccacaagtcg cacgcgatca gcgatgcgat cacgatcgcg tggcggacgc tgctcggcat
     3841 gacccgcaca ccgcaggtgc tggtcttctc gacgatccag ccgatcctgc tcgtgctgat
     3901 gttccgctac gtgttcggcg gcgcgatcca ggtacccggc gtcgagtacg tgaactactt
     3961 gatggccggg gtgttcgcgc aggtcgtcgt gttcggcgcg gtgcagaccg gcatcgggct
     4021 cgcagaagac ttgcagaagg gcctcatcga gcggttccga tcgttgccga tggcgcgttc
     4081 cgccgtgctc gcgggccgca cgcttgccga cctcgtgcgc aacgtcttcg tcgtcgcggt
     4141 catggtcgcg gtcggcttca tcgtcggctg gcgcgtgcac accggcgtgc ccggcttctt
     4201 ggccgggatc ggcgtgctgt tgctgttcgg cttcgcgatg acgtgggtgt tcgcaatcgt
     4261 cggcatgatg gcgacgaacg ccgaggccgc gcaggcgctc gcgttccc
//
LOCUS       DASUGA010000598         4125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_335945, whole genome shotgun sequence.
ACCESSION   DASUGA010000598 DASUGA010000000
VERSION     DASUGA010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335945"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VFX21_11840"
     CDS             complement(<1..213)
                     /locus_tag="VFX21_11840"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HEX5096703.1"
                     /translation="MLVLNEAEALPIFERLRSDTATTTTAAPTVAPAEVSVKVFNGSG
                     VQGQAKNVLDGLGDLGFAVVDPPANAD"
     gene            220..492
                     /locus_tag="VFX21_11845"
     CDS             220..492
                     /locus_tag="VFX21_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HEX5096704.1"
                     /translation="MQYGPGDEAKAQTVLASLGGAGQLVKLDEAAEGADVIVLVGRDF
                     DDLAPATTAPPATTAAPTDASAAGATTTTGPAANPGGTEPMPSAGC"
     gene            530..1825
                     /locus_tag="VFX21_11850"
     CDS             530..1825
                     /locus_tag="VFX21_11850"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015097506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX5096705.1"
                     /translation="MRVAVVGAGYVGLTTAACFAHLGHDVVGADIDTERVNRLNKGEV
                     PILEKGLPELVTAGLASRRLRFVVGAAAAVRDAELVFLCVQTPQAPDGAADMSYVETV
                     AREIAPELRAGTVVVNKSTVPVGSTRFVQRVLSEAGASRDDVSVASNPEFLREGQAVH
                     DFLHPDRIVIGCENPAAAVRVSELYRSVQSPVLVTDPESAEMIKYASNAFLATKISFI
                     NAIANVCEAVDADIREVAIGRGYDARIGFQFLHPGPGYGGSCFPKDVAALLHMARGSG
                     YDFELLAGVDAVNHAQHERMVDKVREMVGGSLTGATVAMWGLTFKAETDDLRDSPALA
                     IARRLLEEGATVRAFDPAAAETAAARVPGLDVRLDVYDASDGADVVALLTEWDEFRWL
                     DFERLRDSMRAPTIVDTRNLLDPTAMRRLGFTYVGVGRR"
     gene            1833..2780
                     /locus_tag="VFX21_11855"
     CDS             1833..2780
                     /locus_tag="VFX21_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEX5096706.1"
                     /translation="MNRVVVTGGAGFLGSHLCRALLARGDRVVAVDNLCTGRRENIDE
                     LEHDGNFELVVADVSQAIPVDGPVTGVLHFASPASPPEYLQMPLETMDVSSLGTRNAL
                     DLARRNRARFLVASTSEIYGNPLVHPQSEDYLGNVDTIGPRAVYDEAKRFTETLTMAY
                     HRLYGLRTSIVRIFNTYGPRLRPADGRVVSNFLVQAAAGDPLTVYGDGRQTRSFCFVD
                     DEIRGILALYDSDIVEPVNIGNPDEYTMLELADLVREVTGSDSTVVYEPLPIGDPVQR
                     QPDITRARELLGWEPTVKLREGLTLMRDWYLEEHARGRA"
     gene            2767..3492
                     /locus_tag="VFX21_11860"
     CDS             2767..3492
                     /locus_tag="VFX21_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5096707.1"
                     /translation="MVARELPPAPKLRKLTIVVPVYNERNTLVEIVRRMRAVELPDNI
                     EREIVVVDDGSTDGTRDVMNQLKDSTVRVILHEHNKGKGAALRTGFAQATGDYVLVQD
                     ADLEYDPNDWPKLIAPVLAGKARVVYGSRFTGERRNMLLLHWIGNRVLSLTTNVLYNT
                     TLSDMETCYKLIDRSLLDGVTLRANRFDIEPEMTAKLLKRGVRIYEVPISYSGREFDE
                     GKKITWKDGFAALWTLVKYRFRD"
     gene            3498..>4125
                     /locus_tag="VFX21_11865"
     CDS             3498..>4125
                     /locus_tag="VFX21_11865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5096708.1"
                     /translation="MTDPAARWAAVVVNFESGPLLSECVQSVLADASAGPAELVVVDN
                     RSRDDSVALMQAACPGVQVIRAPGNVGYARAANLGISATRAPVVAVLNADTRLIEGAG
                     AVMVARLDSQPRVGACGPRLKNTDGTDYPGAREIPGIGVAVMHGLLGLWWPTNPFTRK
                     YRQLDADPSRPRAVDWISGAALWLRRDALDAVGGWDERYFMYLEDTDLC"
BASE COUNT          662 a         1431 c         1382 g          650 t
ORIGIN      
        1 gtccgcgttg gcgggcgggt cgacgacggc gaatccgaga tcacccagac cgtcgagcac
       61 gttcttcgcc tggccctgca caccggaacc gttgaacacc ttgacgctca cctcggccgg
      121 cgccacggtc ggcgccgcag tggtcgtcgt cgccgtgtcg gagcgcagcc gctcgaagat
      181 cggcagcgcc tcggcctcgt tcaacacgag cacaccgagg tgcagtacgg ccccggcgac
      241 gaggcgaagg cccagacggt gctcgccagc ctcggcggtg cgggccaact cgtgaagctc
      301 gacgaagcgg ccgaaggcgc ggacgtgatc gtgctcgtcg gccgcgactt cgacgacctc
      361 gcgccggcga cgacggcgcc gcccgcgacg accgccgcgc ccaccgacgc gagcgcggcc
      421 ggcgcgacga cgacaacggg cccggccgcc aaccccggcg ggaccgaacc gatgccgtcg
      481 gccgggtgtt gatgggcttt cccggccacc ctgcgtaaga ttcgggctca tgcgagtcgc
      541 cgtcgtcggc gccggttacg tgggtctcac cacggccgcg tgctttgccc accttggtca
      601 cgatgtcgtg ggcgccgaca tcgacaccga acgtgtcaat cgcctcaaca agggcgaggt
      661 gccgatcctg gagaagggtc tgcccgagct ggtcaccgcg ggcctggcct cgcggcgact
      721 gcgattcgtc gtcggcgccg cggcagcggt gcgcgatgcg gagctcgtct tcttgtgcgt
      781 acagaccccg caggcacccg acggtgcggc cgacatgagc tacgtcgaga ccgtcgcgcg
      841 cgagatcgca ccggaactgc gcgcaggcac cgtcgtcgtc aacaagtcca cggtgccggt
      901 cggctcgact cgtttcgtgc agcgcgtgct ctccgaagcc ggcgcgtcgc gcgacgacgt
      961 cagtgtcgcg tcgaacccgg agttcctgcg cgagggccaa gccgtccacg acttcttgca
     1021 ccccgaccgc atcgtcatcg ggtgcgaaaa cccggccgcg gcggtgcgtg tctccgagct
     1081 ctaccgctcg gtgcagtcgc cggtgctcgt gaccgatccc gagtcggccg agatgatcaa
     1141 gtacgcgtcg aatgcgttcc tcgcgaccaa gatctctttc atcaacgcga tcgcgaacgt
     1201 gtgcgaggcg gtcgacgccg acattcgtga agtggcgatc ggcagggggt acgacgcgcg
     1261 tatcggtttt cagttcttgc accccggccc gggttacggc gggtcctgct tccccaaaga
     1321 tgtcgccgcg ctgctgcaca tggcgcgcgg ctcggggtac gacttcgaac tgctcgccgg
     1381 cgtcgacgcc gtcaaccacg cgcagcacga gcgcatggtc gacaaggtgc gcgagatggt
     1441 cggcggctcg ctcaccggtg ccacggtcgc catgtggggc ctgacgttca aggccgagac
     1501 cgacgacctg cgggattcgc cggcgctcgc gatcgcgcgt cgcctgctcg aagaaggtgc
     1561 gaccgtgcgc gcgttcgatc cggccgcagc cgagaccgcg gcggctcggg tgcccggtct
     1621 cgacgtacgg ctcgacgtat acgacgcatc cgacggcgcc gatgtcgtcg cgctgctcac
     1681 ggagtgggac gagttccggt ggctcgactt cgagcgactg cgcgattcga tgcgcgcgcc
     1741 gacgatcgtc gacacccgca acctcctcga cccgaccgcg atgcgccggc tcgggttcac
     1801 ctatgtcgga gtagggcgac ggtgacgcgt acatgaatcg agtcgtggtg acgggcgggg
     1861 ccggatttct cggttcacac ttgtgtcgag cgctcctcgc acgcggcgac cgagtcgtcg
     1921 ccgtcgacaa cctctgtacc gggcggcgcg agaacatcga cgagctcgag cacgacggca
     1981 acttcgagtt ggtcgtcgcc gacgtctcgc aagcgatacc ggtcgacggg ccggtcacgg
     2041 gcgtgttgca cttcgccagc ccggcgagcc cgccggagta tctccagatg ccgctcgaga
     2101 cgatggacgt cagctcgctc ggcactcgca acgcgctcga cctggcccgg cgcaaccgcg
     2161 ctcggttcct cgtcgcgtcg acgagcgaga tctacggcaa cccgctcgtc catccgcagt
     2221 ccgaggacta cctcggcaac gtcgacacga tcggcccgcg cgcggtgtac gacgaagcca
     2281 agcgtttcac cgagacgctc acgatggcgt accaccggct gtacgggttg cggacgtcga
     2341 ttgtgcgcat cttcaacacg tacgggccgc ggctgcggcc cgccgacggc cgcgtcgtgt
     2401 cgaacttcct cgtgcaggcg gccgcgggcg atccgctcac ggtctacggc gacggccgcc
     2461 agacccgctc gttctgcttc gtggacgacg agattcgcgg cattctggcg ctctacgact
     2521 ccgacatcgt cgaaccggtg aacatcggca atcccgacga gtacacgatg ctcgagctgg
     2581 cggacctcgt ccgcgaagtc acgggctccg actccaccgt cgtttacgag ccgctgccga
     2641 tcggggatcc tgtccaacgc caacccgaca tcacgcgtgc ccgtgagctg ctgggttggg
     2701 aaccgactgt caagttgcgc gagggtctga cgttgatgcg ggactggtat ctggaggagc
     2761 acgcccgtgg tcgcgcgtga actgccgccg gcgccgaagc tgcgcaagct gacgatcgtc
     2821 gtcccggtct acaacgagcg caacacgctc gtcgagatcg tgcgccgcat gcgcgcggtc
     2881 gaactgcccg acaacatcga gcgcgagatc gtcgtcgtcg acgacggcag caccgacggc
     2941 acccgcgacg tcatgaacca gctcaaggac agcaccgtgc gggtgatcct gcacgagcac
     3001 aacaagggca agggtgccgc gctgcgcacc gggttcgcac aggcgaccgg cgactacgtc
     3061 ctcgtccagg acgccgacct ggaatacgac cccaacgact ggccgaagct gatcgcgccg
     3121 gtactcgcgg gcaaggcccg cgtcgtatac ggctcgcgct tcaccggtga gcgccgcaac
     3181 atgctgctgc tccactggat cggcaaccgc gtcctgtcgc tcaccacgaa cgtgctctac
     3241 aacacgacgc tctccgacat ggagacgtgt tacaagctga tcgaccgttc cctgctcgac
     3301 ggtgtcacgc tgcgcgccaa ccgtttcgac atcgaaccgg agatgacggc gaagctgctc
     3361 aagcggggcg tgcgtatcta cgaggtgccg atctcgtact ccggacgcga gtttgacgaa
     3421 ggcaagaaga tcacgtggaa ggacggcttc gccgcgctgt ggacgctcgt gaagtaccgg
     3481 ttccgcgact agcgcagatg acggatcccg cggcccgctg ggccgcggtc gtcgtgaact
     3541 tcgagtcggg accactcctg tcggagtgcg tgcagtcggt gctcgccgac gcgagcgcgg
     3601 gcccggcgga actggttgtc gtcgacaacc gctcgcgcga cgactccgtc gccctgatgc
     3661 aggccgcgtg tcccggtgta caagtgattc gcgcgcccgg caacgtcggt tacgcgcgcg
     3721 cggccaacct gggaatctcg gcaacacgtg cgcccgtcgt cgcggtgctg aatgccgata
     3781 cacgactcat cgagggagcc ggcgcggtca tggtggcgcg cctcgactcg caaccgcgtg
     3841 tcggcgcgtg cggcccgcgc ctcaagaaca ccgacggaac cgactacccg ggtgcgcgcg
     3901 agatcccggg catcggcgtc gccgtcatgc acggcctgct cgggctctgg tggccgacga
     3961 acccgttcac ccgcaagtac cgccaactcg acgccgatcc gtcgcgtcct cgcgccgtcg
     4021 actggatctc gggcgccgcg ctgtggctgc ggcgcgacgc gctcgacgcc gtcggcgggt
     4081 gggacgagcg gtacttcatg tacctcgagg acaccgacct gtgct
//
LOCUS       DASUGA010000599         3238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_160455, whole genome shotgun sequence.
ACCESSION   DASUGA010000599 DASUGA010000000
VERSION     DASUGA010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160455"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VFX21_11870"
     CDS             <1..366
                     /locus_tag="VFX21_11870"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX5096709.1"
                     /translation="GVAVLVKRAFDATVDDVRWAEALILGTPENFGYMSGALKDFFER
                     IYYVLLEETQGLPYALFVKGGHDGEGAIRSIERIVAGLKWNQVQPPVLVVGDLDDDAL
                     EQCHELGMTIAASLEAGLF"
     gene            complement(680..1627)
                     /locus_tag="VFX21_11875"
     CDS             complement(680..1627)
                     /locus_tag="VFX21_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5096710.1"
                     /translation="METRRIGSLDASVVGLGCNNFGMRIDEDAAKAVVDAAIDAGITL
                     FDTADIYGATKSEEFLGRALGAQRDQVLIATKFGGPIDDQRKGGASADYITQACDDSL
                     RRLGTDRIDLYQLHFPDEHTMLEETLAALDELVRAGKVLEIGCSNFSKELIEESERIS
                     ADAKSARFASVQNEYSLLRRLPEQNGVLDACAKFGIAFIPYFPLASGALTGKYRRGEE
                     PPAGTRLANVPAERRDATLSDKNYDRIEALDGWARDHGHTLLELAFAWLLAHQPVASV
                     IAGATKPEQVRANAAAADWQLTSADVAEIDAVIARAKQE"
     gene            complement(1637..3202)
                     /locus_tag="VFX21_11880"
     CDS             complement(1637..3202)
                     /locus_tag="VFX21_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012855050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5096711.1"
                     /translation="MPNATYNLADLWEMLCDAGPDAECLVAGNTRHTRRSLDRAANQI
                     ANHLVAEGIKPGDRVGIYARNCAEYVEALLGCWKCGAVPVNINWRYVPDELRYVIDDA
                     ELRAIIVQDEYVPVLEKLEFDAIVRFGAWASASAERAFDDVPRSSDDVYMLYTGGTTG
                     MPKGVMWRHEDFFYACCLGGSALEPIKEPAEIVKNVDPLLKLSPLVLGPLMHGGGQWL
                     TLIAIYGGNKALLYCERSFDAAAVLDFAVAEGATTIGIIGDAMARPLAEAVLAEPGRW
                     DVSAIFGLGNGGAMLSAAVKEQLSRAFPTAVLNDSYGATETGAAGSEVGASTSRDRPA
                     FSTDGRTWVLDPETLERLEPGSGVEGLFARTGNIPLGYWKDPEKTAATFRVDASGTRW
                     VVPGDWATIDEEGRIVLFGRGSGCINTGGEKVFPEEVEAAIRSHPDVFDAVVVGMPDD
                     RFGQKVVALVKLRDGAEALTLAALQEHCREKIAGYKVPRELLLGEAPRTNTNKPDYAT
                     ARQIAEASLDRAP"
BASE COUNT          548 a         1098 c         1027 g          565 t
ORIGIN      
        1 ggcgtcgcgg ttctcgtgaa gcgtgcgttc gacgcgacgg tcgatgacgt gcgttgggcg
       61 gaggcgctca tcctgggaac gcccgagaac ttcggataca tgagcggggc gctcaaggac
      121 ttcttcgagc gcatctacta cgtgctcctc gaagaaacgc aagggttgcc gtacgcgttg
      181 ttcgtgaagg gcggtcacga cggcgaaggc gcgatccgca gcatcgaacg catcgtcgcg
      241 ggcctgaagt ggaatcaggt gcagccaccc gtgctcgtcg tcggcgatct cgacgacgac
      301 gcactcgagc aatgccacga gctcggtatg acgatcgcgg caagcctcga agccggcttg
      361 ttctgacacg catgttgcga atgagaagcg agtacgcagg agacgacgca agttgtcgtc
      421 atcgcccctt gtccaacggc tgcgtcggat tgaataattc tgcccacggg gttggatgcg
      481 ggggctgcac cagcaggagc ggcgtggcgc cttcgtccga cccctcaggt gttcgttgtg
      541 gggatggagc cagggggcca catgctgcaa cggatcatgg actggtgcgt tcccgacgac
      601 gcgctgaact tcgtactcga aacccggtcc tgggcaggct cgttgctcga accgtacgag
      661 gcaacgccga ccgagaccgc tactcctgtt tcgctcgcgc gataacagcg tcgatctccg
      721 cgacgtccgc tgacgtcaac tgccagtccg cagcggccgc gttcgcgcgg acttgctcgg
      781 gcttcgttgc gcccgcgatg acgctcgcca cgggttggtg cgcgagcagc cacgcgaacg
      841 cgagctccaa caacgtgtgc ccgtggtcgc gagcccagcc gtcgagtgct tcgatgcggt
      901 cgtagttctt gtccgacagc gttgcgtcgc ggcgttcggc gggcacgttg gcgagacggg
      961 tgccggccgg cggttcttca ccgcggcggt acttcccggt gagcgcgccg ctcgcgagcg
     1021 ggaagtacgg aatgaacgcg ataccgaact tcgcgcaggc gtcgagcacg ccgttctgtt
     1081 cgggaagccg gcgcaagagg ctgtactcgt tctgcacgga ggcgaagcgg gccgacttgg
     1141 cgtcggccga aatgcgctcg ctctcctcga tgagctcctt ggagaagttg gagcagccga
     1201 tctcgagcac cttgcccgcc cgcacgagct cgtcgagcgc ggcgagcgtc tcttccagca
     1261 tcgtgtgctc gtcggggaag tgcagctggt agaggtcgat gcgatcggtg ccgagccggc
     1321 gcagggaatc gtcgcaggcc tgagtgatgt agtcagccga cgcaccgccc ttgcgctgat
     1381 cgtcgatcgg cccgccaaac ttcgtggcaa tcaaaacctg atcgcgttgg gcgccgagcg
     1441 cccgcccgag gaactcctcc gacttggtcg cgccgtaaat gtcggccgta tcgaacagcg
     1501 tgatgccggc gtcaatggcg gcgtcgacaa ccgccttcgc ggcatcctcg tcgatccgca
     1561 tgccgaagtt gttgcaaccg agccccacca ccgaggcgtc gagcgaccca atcctgcgcg
     1621 tctccatgcc cgcaggctac ggcgcgcgat cgagtgacgc ctccgcgatc tggcgcgcgg
     1681 tcgcatagtc gggtttattg gtgttcgtcc gtggtgcttc acccagcagc aactcgcggg
     1741 gcactttgta gcccgcgatc ttctcgcggc agtgctcctg cagcgccgcc agcgtcagcg
     1801 cttcggcacc gtcgcgcagc ttcaccaacg cgaccacctt ctggccgaag cgatcgtcgg
     1861 gcatgccgac gacaaccgcg tcgaacacat cgggatgcga acggatcgcg gcctcgacct
     1921 cctcgggaaa caccttctca ccgccggtgt tgatgcagcc cgaaccgcgg ccgaacaaca
     1981 cgatgcggcc ttcctcgtcg atcgtcgccc agtcgccggg cacaacccac cgggttccgg
     2041 acgcgtcgac ccggaaggtc gcggcggtct tctcggggtc cttccagtat ccgagcggaa
     2101 tgttgccggt gcgcgcgaac aaaccttcga cacccgatcc cggttccagc cgctccaatg
     2161 tttccggatc gaggacccac gtgcggccgt cggtcgagaa cgccggccga tcgcgcgacg
     2221 tcgacgcgcc cacctcgctg cccgccgccc ccgtttcggt cgcgccgtac gagtcgttca
     2281 gcaccgccgt cggaaacgcc cgcgacagtt gctctttcac tgccgcactc aacatcgcgc
     2341 cgccattgcc gaggccgaag atcgcggaca cgtcccaacg gccgggctcc gccaacaccg
     2401 cttcggccaa cggccgcgcc atcgcgtcac cgataatgcc gatcgtcgtc gcaccttcgg
     2461 cgaccgcaaa gtcgagcact gcggccgcgt cgaacgatcg ctcgcaatac agcaatgcct
     2521 tgttgccgcc gtagatcgcg atcagtgtca gccactgccc gccaccgtgc atgagcggac
     2581 cgagcaccag tggcgagagc ttcagcagcg gatcgacgtt cttgacgatc tccgccggct
     2641 ccttgatcgg ttcgagcgcc gagccgccca gacagcacgc atagaagaaa tcttcgtgcc
     2701 gccacatgac ccccttcggc atgcccgtgg tgccgccggt gtagagcatg tagacgtcgt
     2761 cgctcgatct cggaacgtcg tcgaacgccc gctccgcgga agccgatgcc cacgcgccga
     2821 accgcacgat cgcgtcgaac tcgagcttct cgagcacggg cacgtactcg tcctgcacga
     2881 tgatcgctcg gagctctgcg tcgtcgatca cataccgcag ctcgtccggg acgtagcgcc
     2941 agttgatgtt gaccggcacc gcgccgcact tccaacagcc gagtaacgcc tcgacgtact
     3001 ccgcgcagtt gcgggcgtag atgcccaccc ggtctccggg cttgatgcct tccgcaacga
     3061 gatggttggc aatctggttc gcggcccgat caagactgcg gcgcgtgtgg cgagtgttgc
     3121 ccgcgaccag acactcggcg tccgggcccg cgtcgcacaa catctcccaa aggtctgcaa
     3181 ggttgtaagt cgcattcggc acggcgcgat tgtgacgccg cgcctaggct gcgcgcca
//
LOCUS       DASUGA010000600         2873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_630453, whole genome shotgun sequence.
ACCESSION   DASUGA010000600 DASUGA010000000
VERSION     DASUGA010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630453"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="VFX21_11885"
     CDS             complement(<1..155)
                     /locus_tag="VFX21_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014927676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEX5096712.1"
                     /translation="MPYSWFVDAALKALAEPRRREILRLVWSQELPATDIADSFRDVT
                     RPAISQHL"
     gene            200..619
                     /locus_tag="VFX21_11890"
     CDS             200..619
                     /locus_tag="VFX21_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007534221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX5096713.1"
                     /translation="MDKKVQQLLDKVTPAKRKRDAETMLEMMGRVTGQPPVVERSAVG
                     YGRYHYKYDSGREGDAPAAGFAPRKPALVVYLMDGVSSHAKALAKLGPHTSTVGCLYI
                     KDLTQCDLAVLEKIVAKSYKTLTAGTYYGLRARDGRD"
     gene            663..2168
                     /locus_tag="VFX21_11895"
     CDS             663..2168
                     /locus_tag="VFX21_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX5096714.1"
                     /translation="MVEGIVPRWEWRTFGFGAALDAAERRLGAMQAEKTQDSDEVYLL
                     SVHNDTSCKLRASQLDVKQLQAVNADDLELWKPIMKADFPLTAADATTVFNALSHPVP
                     ALARATYTLEQFAELIEHDPRLCVVGVHKTRTHYLVDDCMVEKTVLATKHGAVHTIAI
                     ESPDPGLVLATIAKLGLDGRRNVNVPRGLKTLAHFDAQRYAVIDIGTNSVKFHLGEVR
                     ADGTVRTIADRADVTRLGEGLDEHRRLDDAAMARTADAVVALFEEARHAQVAGIVVVG
                     TAGLRSAENREEFDELLKRRIGVTVEIISGEQEGRLAYVAAISALPLARGQLVVFDSG
                     GGSTQFTFGRGADVSERFSVDIGAVRITERCGLAGKVTREQLDDALTVIATELQRLDG
                     RATPDSVVGMGGTLTNLAAVHHQLAEYDPEIVHGTVLDLAEIDRQIERYRTLDASERR
                     TIVGLQPNRAEVILGGACIVRTILAKLGRGAVTVTDRGVRHGLFIERFAEA"
     gene            complement(2169..>2873)
                     /locus_tag="VFX21_11900"
     CDS             complement(2169..>2873)
                     /locus_tag="VFX21_11900"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_037116981.1"
                     /note="catalyzes the formation of ornithine and
                     carbamylphosphate from citrulline in the arginine
                     catabolic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase subunit F"
                     /protein_id="HEX5096715.1"
                     /translation="RMYDAIEYRGANQVDVEALAEYAGVPVYNGLTAEWHPTQMLADF
                     LTMHEASNKPYDALTYAFVGDCRFNMGRSLLVMGALMGSDVRLAGPKELQPPPDVVKI
                     AKEIAGRTGATITITDDAAAGVKGADFVHTDVWVSMGEAKDVWDQRVSLLKPYQVNAD
                     LLKASGNSRIKFMHCLPAFHDTNTVVGREMMEHTGMTSGLEVTNDVFESGASIVFDQA
                     ENRLHTIKAILVATLG"
BASE COUNT          490 a          983 c          960 g          440 t
ORIGIN      
        1 aggtgctgcg agatcgcggg ccgggtgacg tcacggaacg agtccgcgat gtcggtcgcg
       61 gggagctcct gcgaccacac caggcgcagg atctcccggc gccggggctc ggccagtgct
      121 ttcagcgcag cgtccacgaa ccaagagtaa ggcattgctt acgtaagtgt caactaaaga
      181 ttcgtggcac gctggagccg tggacaagaa ggtgcagcag ctgttggaca aggtgacgcc
      241 ggccaagcgc aagcgcgatg ccgagacgat gctcgagatg atgggtcggg tcaccggcca
      301 accgccggtc gtcgaacgca gcgcggtcgg ctacgggcgg taccactaca agtacgacag
      361 cggccgcgag ggcgacgcgc ccgcggccgg cttcgcgccg cgtaagccgg ccctcgtcgt
      421 gtacctgatg gacggcgtca gttcgcatgc caaggcgctt gcgaagctgg ggccgcacac
      481 gagcaccgtc ggttgcctct acatcaagga tctgacgcag tgcgatctcg cggtgctcga
      541 gaagatcgtc gcgaagtcgt acaagacgct gaccgccggc acctactacg gattgcgcgc
      601 ccgcgacggc cgcgactgag acgcgctact cgccggcaac cgcgacgtga catgctgcgg
      661 ccatggtcga aggcatcgtg ccgcggtggg agtggcgtac gttcggcttc ggcgccgcac
      721 tcgacgccgc cgaacgacgc ctcggcgcca tgcaagccga gaagacccag gacagcgacg
      781 aggtctacct gctctccgtg cacaacgaca cgtcctgcaa gctgcgcgcg tcgcagctgg
      841 acgtgaagca gctgcaagcg gtgaacgccg acgatctcga gctgtggaag ccgatcatga
      901 aggccgactt cccgctcacg gccgccgacg cgacgaccgt gttcaacgct ttgtcgcatc
      961 cggtgccggc actggcgcgc gccacctaca cgctcgagca gttcgccgag ctcatcgagc
     1021 acgacccgcg cctgtgtgtg gtcggcgtgc acaagacgcg cacgcactac ctcgtcgacg
     1081 actgcatggt cgagaagacg gtcctggcga cgaagcacgg agcggtgcac acgatcgcca
     1141 tcgagtcacc tgatcccggc ctcgtgctgg cgacgatcgc gaagctcggt ctcgacggcc
     1201 ggcgcaacgt caacgtcccg cgcggcctga agacgctcgc ccacttcgac gcgcagcgct
     1261 acgccgtcat cgacatcggg accaactcgg tcaagttcca tctcggtgaa gtgcgggccg
     1321 acggcactgt gcgcacgatc gcggaccggg ccgacgtcac ccggctcggc gaagggctcg
     1381 acgagcaccg ccgcctcgac gacgccgcga tggcgcgcac cgcggacgcc gtcgtcgccc
     1441 tgttcgagga ggcgcgccac gcgcaggtgg cggggatcgt cgtcgtcggc accgccgggc
     1501 tccgcagcgc ggagaaccgc gaggagttcg acgagctcct gaagcgccgg atcggcgtca
     1561 cggtcgagat catcagcggc gaacaagaag gacgcctcgc gtacgtcgcc gcgatctccg
     1621 ccctcccgct cgcacgcggg cagctcgtcg tgttcgattc cggcggcggc agcacgcagt
     1681 tcacgttcgg ccgcggtgcc gacgtctcgg aacgcttcag cgtcgacatc ggcgcggtgc
     1741 gcatcaccga gcgctgcggc ctggcgggca aggtcacacg cgaacagctg gacgacgcac
     1801 tgaccgtcat cgcgacggag ctgcaacgcc tcgacggtcg cgcgacaccc gactcggtgg
     1861 tcggaatggg cggcacgttg acgaacctcg ccgccgtgca tcaccaactc gccgagtacg
     1921 accccgagat cgtgcacggt acggtgctcg acctcgccga gatcgaccgc cagatcgagc
     1981 gctaccgcac gctggacgca agcgagcgcc gcacgatcgt cgggctccaa ccgaaccgcg
     2041 ccgaggtcat cctcggtggc gcgtgcatcg tgcgcacgat cctcgccaag ctcggacgcg
     2101 gcgccgtcac cgtgaccgac cgcggcgtgc gccacggcct tttcatcgaa cgcttcgccg
     2161 aagcctgatc agccgagcgt cgctacgagg atcgccttga tcgtgtgcag ccggttctcg
     2221 gcctggtcga acacgatgct cgcgcccgac tcgaacacgt cgttcgtgac ctcgagaccg
     2281 ctcgtcatgc ccgtgtgctc catcatctcc cgcccgacga ccgtgttcgt gtcgtggaac
     2341 gcgggcaggc agtgcatgaa cttgatgcgc gagttgcccg acgccttcag caagtccgcg
     2401 ttgacctgat acggcttgag caacgagacg cgctgatccc agacgtcctt cgcctcgccc
     2461 atcgacaccc acacgtcggt gtgcacgaag tcggcacctt tgacgccggc ggccgcgtcg
     2521 tcggtgatcg tgatcgtcgc gccggtgcgg cccgcgatct ccttggcgat cttcacgacg
     2581 tccgggggcg gctgcagttc cttcggtccg gcgaggcgca cgtcgctgcc catcagcgcg
     2641 cccatgacga gcagggaacg gcccatgttg aagcggcagt cgccgacgaa ggcgtacgtc
     2701 agcgcgtcgt agggcttgtt gctcgcctcg tgcatggtga ggaagtcggc gagcatctgg
     2761 gtggggtgcc actcggcggt cagaccgttg tatacgggca cgcccgcgta ctcggcgagg
     2821 gcttcgacgt cgacctggtt cgcgccgcga tattcgatcg cgtcgtacat gcg
//
LOCUS       DASUGA010000601         4932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_529118, whole genome shotgun sequence.
ACCESSION   DASUGA010000601 DASUGA010000000
VERSION     DASUGA010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529118"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="VFX21_11905"
     CDS             complement(<1..965)
                     /locus_tag="VFX21_11905"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5096716.1"
                     /translation="MDEAGGFLIPGAFESFRLDLGLTYTQASIVLVAPAPGALLGNGL
                     SIAVDHRSRRAITATGAFGFAASLCSVAVTPGFAACVAAAFVLGAAATLTIRGSDVSL
                     VDMAGDAPTTMIARSHVFGAAGDLFGPALLVLVAATGLGWRVAFATFGVLAFAYAGWL
                     ASLRFPPPPPAPDGHRVRDSLRDITRDRRVWWCAFVALLLGPLDEPFLAFLIATLERE
                     QHLSAWLATAIVVSSIAGNLVGFLQTGRAGHTPAPLAMASNAAILACTTAIAVVAPWP
                     VVIVAIFVSGWATAHFFIALMDRIVALHPGRLGTVQAVITTVEFSG"
     gene            1024..1716
                     /gene="aat"
                     /locus_tag="VFX21_11910"
     CDS             1024..1716
                     /gene="aat"
                     /locus_tag="VFX21_11910"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX5096717.1"
                     /translation="MILATAPTLPAADEAGCQYAPVPIEPAPSRWALPDPARAGRSDV
                     VALGGDLEPGTLLDAYRRGMFPMRLEGELAWWSPDPRGILPLDGFHISRSLRRSIPKF
                     GIRVDTAFEAVMRECGDPRRPHGWIDESFVAAYVRLHEMGWTHSVEAWRGDALVGGVY
                     GVRINGLFAGESMFSRVPDASKVALRAAVELLRTDGAVLFDVQWTTPHLASLGAVDVS
                     RAEYLRLLASAV"
     gene            1713..2072
                     /gene="arsC"
                     /locus_tag="VFX21_11915"
     CDS             1713..2072
                     /gene="arsC"
                     /locus_tag="VFX21_11915"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672581.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEX5096718.1"
                     /translation="MSEPVQIFHNPSCSKSRGALEILNDRGVDPTVVKYLDTPPDRAT
                     IERILDAIDDEPQALVRTGDQKFKDAGYTKADVATRAQVVDVLLKHPEVMERPVVFVG
                     KKAVIARPSERVLELLD"
     gene            2084..2338
                     /locus_tag="VFX21_11920"
     CDS             2084..2338
                     /locus_tag="VFX21_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008360287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096719.1"
                     /translation="MSFLLSVTFACGLFIAVAHVMNQRFVAPVYDVAANLIAFGCAVA
                     ASSLLGHPVPAMLSAVAVVCWLVLARRTILDSRRRSIDSN"
     gene            2389..3639
                     /locus_tag="VFX21_11925"
     CDS             2389..3639
                     /locus_tag="VFX21_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013426165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5096720.1"
                     /translation="MTLPSVEDVRAEVKAWLEENWNPDLTVGEWWDILARSGYAAPTF
                     PEDCWGKGWPRDLAMAVAEVIAEHGAIGPPAGLGYLLAAPTIVAHGNERQKQDDLLRI
                     LNGQDAWCQLFSEPGAGSDLASLQTKAIKDGDEWIITGQKVWTSTAQQSNVGMLIART
                     DPDLPKHKGITYFRFDMTQPGVEIRPLREMTGRALFNEVFIDGARVPDSDIIGGLNNG
                     WAATNTTLMAERAGLGTGGTGAAGNAFPGPIAGQLGHRAGDHVGQVRTGGTGGGGASA
                     AQRLAALAKELGKSDEPVIRQKLAKLYTMQKIGRYSALRAKTASQRTGGEPNIAKLMM
                     SDLLRLQREVGNEIIGANGMLMGADTPGGGMVQELTLFSPGPSIYGGTDQVQRNILGE
                     RVLGLPKEPGPAKDTPFRELLVSR"
     gene            3662..4552
                     /locus_tag="VFX21_11930"
     CDS             3662..4552
                     /locus_tag="VFX21_11930"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5096721.1"
                     /translation="MKVGIALPTMVPGLTRDILLSWMREVDEGPFSVLACGERVAYPN
                     QDMMSVLAAAAAVTQRVAIEATVSLVPMHSAVHVAKQAATIDVLSNGRFVLGVGVGGR
                     DEDYRAIGASFARRHARLDEQVATMKRVWRGEALADDVSPVGPPPVQPGGPKVLTASM
                     GPKSMARAAQWADGIAGFDTGPNPAGIGGTFAAFTSAWRAAGREGEPYRQTSFWFGLG
                     ANAATEVPAYAYRYLRIFGHEMASALSQGCCAVSEDAVRDLLRQVADTGCDEAILVAT
                     TSDVDDLRRAVQLIGVSEFA"
     gene            4589..>4932
                     /locus_tag="VFX21_11935"
     CDS             4589..>4932
                     /locus_tag="VFX21_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096722.1"
                     /translation="MKRLGLAVLVLALGLAACGGDDDDNATSSPSTEAVSKTGDDADA
                     EHAAEHAAGEHDMEGMDASEHDMADMEDAVDDRGFAKLENGEQHAHSFTQAISKEDRV
                     ELARQLVLARETA"
BASE COUNT          786 a         1620 c         1793 g          733 t
ORIGIN      
        1 ccgctgaact cgaccgtggt gatgacggct tgcaccgtgc cgagccggcc ggggtgcagc
       61 gcgacgatcc ggtccatgag cgcgatgaag aagtgggcgg tcgcccaacc ggacacgaag
      121 atcgcgacga tgacgaccgg ccacggggcg acgaccgcga ttgcggtggt gcacgcgagg
      181 atcgccgcgt tgctcgccat tgcaagcggc gccggcgtgt ggccggcgcg gccggtctgg
      241 aggaagccca cgaggttgcc ggcgatgctc gagacgacga tcgccgtggc cagccacgcc
      301 gacaggtgct gctcgcgttc gagcgttgcg atgaggaacg cgaggaacgg ttcgtcgagc
      361 ggtccgagga gcagcgcgac gaacgcgcac caccacacgc gccggtcgcg tgtgatgtcg
      421 cgcaacgagt cgcggacgcg gtggccgtcg ggcgccggcg gtggcggcgg gaaccggagc
      481 gacgccagcc atccggcgta ggcgaacgcg agcacgccga aggtcgcgaa cgcgacgcgc
      541 cagccgaggc cggtggcggc gacgagcacg agcagtgcgg gtccgaacag gtcgccggcc
      601 gcgccgaaga cgtggctgcg cgcgatcatc gtcgtcggcg cgtcgcccgc catgtcgacg
      661 agcgagacgt cgctcccgcg gatcgtcagc gtcgcggcgg caccgaggac gaatgcggcc
      721 gcgacgcacg ccgcgaatcc gggcgtgact gcaaccgaac acaacgacgc cgcgaacccg
      781 aacgcgccgg ttgcggtgat ggcacggcgg cttcggtggt cgaccgcgat ggagagtccg
      841 ttgccgagga gcgcgcccgg cgcgggtgcg acgaggacga tgctcgcttg cgtgtacgtc
      901 agcccgaggt caagccggaa tgactcgaac gcgccgggga tgaggaaacc gccggcctcg
      961 tccacgaacc gcgcggtgca caacgcgagc accagcgcgg cagggaagcg acgtcaatag
     1021 tgcgtgatcc tcgccaccgc gccgacgtta ccggctgccg acgaagcggg ctgtcaatat
     1081 gcgcccgtgc cgatcgagcc cgcgcccagc cgttgggcgc ttcccgatcc cgcgcgtgcg
     1141 ggccgcagcg acgtcgtcgc gctcggcggt gacctcgagc cggggacgtt gctcgacgcg
     1201 taccggcgcg gcatgttccc gatgcggttg gagggcgagc tcgcgtggtg gtcgcccgat
     1261 ccgcgcggca ttctcccact cgacggtttc cacatcagcc gatcgttgcg ccgcagcatc
     1321 cccaagttcg ggatccgcgt cgacaccgca ttcgaagcgg tgatgcgcga gtgcggtgat
     1381 ccgcgccggc cgcacggatg gatcgacgag tcgttcgtgg cggcgtacgt ccgcctgcac
     1441 gagatgggct ggacgcacag tgtcgaggcg tggcggggcg atgcgctcgt cggcggcgtc
     1501 tacggcgtgc gcatcaatgg tctgttcgcg ggcgagtcga tgttctctcg ggtgccggac
     1561 gcgtcgaagg tcgcgctgcg cgccgcggtc gagctcctgc ggacggacgg tgcggtgttg
     1621 ttcgacgtgc agtggacgac gccgcacctt gcgtcgctcg gcgcagtcga cgtctcgagg
     1681 gccgagtacc tgcggttgct agcgtcggca gtatgagtga gcccgtacag atcttccaca
     1741 acccgtcgtg ctccaagtcg cggggcgcgc tcgagatcct caacgaccgc ggtgtcgacc
     1801 cgaccgtcgt gaagtatctc gacacgccgc ccgaccgcgc cacgatcgag cgcatcctcg
     1861 acgccatcga cgacgaaccg caggcgttgg tgcgaaccgg cgaccagaag ttcaaggacg
     1921 ccggctacac caaagccgac gtcgcaacgc gtgcgcaggt cgtcgacgtg ttgctgaaac
     1981 acccggaagt gatggagcgt ccggtcgtgt tcgtcggcaa gaaggccgtg atcgcccgcc
     2041 catcggagcg cgtgctcgaa ctgctcgact aacgacgacc gtggtgagtt tcctcctgtc
     2101 cgtgaccttc gcgtgcggtc tcttcatcgc cgtcgctcac gtcatgaacc agcgctttgt
     2161 cgccccggtg tacgacgtcg ccgcgaacct catcgcattc ggatgtgccg tcgcggcctc
     2221 ctcgctcctc ggtcacccgg tgcccgcgat gctctccgcc gtcgccgtcg tgtgctggct
     2281 cgtcctcgcg cggcggacga tcctcgacag ccgccgccgg tcgatcgatt cgaactgagc
     2341 cacgacccga ctcacggcga ttcggggccg cccggtaatc tcccgcgcat gacattgccg
     2401 agcgtcgagg acgtgcgcgc cgaggtcaag gcgtggctgg aagagaactg gaaccccgac
     2461 ctgacggtgg gtgagtggtg ggacatcctc gcccgttcgg ggtacgccgc gccgaccttc
     2521 cccgaggact gctggggcaa gggctggccg cgcgacctgg cgatggccgt cgccgaagtg
     2581 atcgccgagc acggtgcgat cggtccgccc gcgggtctcg gctacctgct cgcggcgccg
     2641 acgatcgttg cccacggcaa cgagcgtcag aagcaggacg acttgctgcg catcctcaac
     2701 gggcaggacg cgtggtgcca gttgttctcc gagccgggcg ccggttccga cctcgcgagc
     2761 ctgcagacga aggcgatcaa agacggtgac gagtggatca tcaccggcca gaaggtgtgg
     2821 acgtcgaccg cgcagcagtc gaacgtcggc atgctgatcg cgcgcaccga tcctgatctg
     2881 ccgaagcaca agggcatcac gtacttccgc ttcgacatga cgcagccggg cgtcgagatc
     2941 cgtccgttgc gcgagatgac cggccgcgcg ctgttcaacg aagtgttcat cgacggcgcg
     3001 cgtgttcccg actccgacat catcggtggg ctcaacaacg gttgggccgc gacgaacacg
     3061 actttgatgg ccgagcgtgc gggtctcggt accggtggta cgggtgcggc cggcaacgcg
     3121 ttcccgggtc cgatcgcggg tcagctcggg caccgcgccg gcgatcacgt cgggcaggtg
     3181 cgcaccggcg gcaccggcgg cggcggtgcg tcggccgcgc agcggttggc cgcgctcgcg
     3241 aaggagctgg gcaagagcga cgagcccgtc atccgccaga agctcgcgaa gctctacacg
     3301 atgcagaaga tcggccggta ctcggcgttg cgcgccaaga ccgcgagcca gcgcaccggt
     3361 ggcgagccca acatcgcgaa gctgatgatg agcgacttgt tgcgcctgca gcgcgaggtc
     3421 ggcaacgaga tcatcggcgc caacggcatg ctcatgggcg ccgacacgcc cggcggcggc
     3481 atggtgcagg agctcacgtt gttctcgccc gggccgtcga tctacggcgg taccgaccag
     3541 gtgcagcgca acatcctcgg cgagcgcgtg ctcggactgc cgaaggagcc cggccccgcc
     3601 aaggacaccc cgttccgcga actgctcgtc agccgctgag cgacgcgctc ggcgcttcgg
     3661 catgaaggtc ggcatcgcgc tcccgacgat ggtgcccggg ctgacgcgcg acatcttgtt
     3721 gtcgtggatg cgcgaggtcg acgaggggcc gttctcggtg ctcgcgtgcg gcgagcgtgt
     3781 cgcctacccg aaccaagaca tgatgtcggt gctcgcggcg gctgcggccg tgacgcagcg
     3841 agtcgcgatc gaagcgacag tgagcctcgt gccgatgcac tcggcagtgc acgtcgcgaa
     3901 gcaggcggcg acgatcgacg tgttgtcgaa cggtcgcttc gtgttgggtg tcggcgtcgg
     3961 tggccgcgac gaggactacc gcgcgatcgg tgcgtcgttc gcgcgccggc acgcgcgtct
     4021 cgacgaacag gtcgcgacga tgaagcgggt gtggcgcggc gaagcgctcg ccgacgacgt
     4081 ctcgccggtc ggtccgccac ctgtgcagcc gggcgggccg aaggtgttga ccgcgtcgat
     4141 ggggccgaag tcgatggcgc gcgcggcgca gtgggcggac gggatcgcgg gattcgacac
     4201 ggggccgaac ccggcgggca tcggtggcac atttgccgcg ttcacgtccg catggcgcgc
     4261 cgcgggacgt gagggcgagc cctaccggca gacgtcgttc tggttcggcc tcggcgcgaa
     4321 cgcggcgacc gaagtgcccg catacgcgta ccgctacctg cgcattttcg gccacgagat
     4381 ggcgtccgcg ctgtcgcagg gctgttgcgc ggtgtcggag gacgcggtgc gggatctgct
     4441 ccggcaggtg gccgacaccg gttgcgacga agcgatcctg gtcgcgacga cgtccgacgt
     4501 cgacgacctc cgccgcgcgg tgcagctgat aggagtttcc gaattcgcct aaggttcgcg
     4561 gcgggcgtca gtccgtcagg gggaatccat gaagcgcttg ggcttggccg ttctcgtgtt
     4621 ggcactcggg ctggctgcat gcggaggtga tgacgacgac aacgcaacgt cgtctccctc
     4681 gacggaagcg gtgagcaaga ccggcgacga cgccgatgcc gagcacgcgg cggagcacgc
     4741 cgcgggcgaa cacgacatgg agggcatgga cgcgtccgag cacgacatgg ccgacatgga
     4801 agacgcggtc gacgaccgcg gcttcgccaa gctcgagaac ggcgaacagc acgcgcactc
     4861 gttcacgcag gcgatctcga aggaagatcg cgtcgagctc gcccgacagt tggtgctcgc
     4921 gcgtgagacc gc
//
LOCUS       DASUGA010000602         3267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_512034, whole genome shotgun sequence.
ACCESSION   DASUGA010000602 DASUGA010000000
VERSION     DASUGA010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512034"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VFX21_11940"
     CDS             complement(<1..1002)
                     /locus_tag="VFX21_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEX5096723.1"
                     /translation="MSFKVIQFATGNVGRLALQGILEHPDLELVGLWVHSADKAGKDA
                     GELCGRPSTGVLTTNDFEEILALEADAVSHNSTGDLRPAEAAAEIAALLESGKNVVSS
                     AVISLLHPGTADRGMVDQLEAACAKGQTSCFSSGIDPGFANDLLPLTFSGFCARVDSI
                     RVAEILNYDTYDQATVLFDTMGFGQPMDQVPFLLMPGVLGYAWGSVIHLMAEGLGVEV
                     DEIRERYDRVPAPETFEIAAGTVEAGTVAGLRFEIEGIVDGEPVLTVEHVTRLRDDLA
                     PEWPAPPEGGGYRIEVKGSPSFRCELSMLGEDGDHNTGGLLATAMRLLNAIPAVCAAP
                     "
     gene            1001..2101
                     /locus_tag="VFX21_11945"
     CDS             1001..2101
                     /locus_tag="VFX21_11945"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5096724.1"
                     /translation="MSEISHTGAVTVLRRLGAVLAVVAVALLTAATTAASAAAKDPFA
                     ALDRSMRDRVKDDDLAGAVLLVVRDDAVIHATEFGALDADSVIPIASASKWLTSATLM
                     TLVDDGKVSLDESIATYLPEFKGSNKKKAITVRQLLAHTHGLPYASCIGDPTTTTANC
                     TAAIANGPDPVTRPGKVFSYGGVGYEVAARIIEVISGQSFEDAFETRIARPLGMTHTS
                     FDEYQGQRFRHPQPSGSAVSSVDEYAKFLAMLTAGGESANGRVLTEDSVAEIERDQVR
                     GIDTHRDAAVQITNIPTYGLGVWRDVASKTDDIEVVSGSGAYGFYPWIDRRNGNYGIV
                     GVADLTNGAEHAVPASQRQARVAWRAAAHWVP"
     gene            complement(2116..>3267)
                     /locus_tag="VFX21_11950"
     CDS             complement(2116..>3267)
                     /locus_tag="VFX21_11950"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5096725.1"
                     /translation="APVWWHPEHRGTDRLGGSFWVVSKHADVQAVSRDPARFSSLDGP
                     SLPTLPPEQRGAMVVSMDPPDHTRMRRLVSAGFTPRMITKLEDQARAWASAIVDRALE
                     QKQCNFVHEVAYQLPMHMIADIVGIPVSDREWLFDRVNVFLLSTDPRSRLSRDEINAI
                     QAEMFGYAQQLGEEKRRVPADDVWTTLIQAEIEHDDGERTRLSPVELDLFFIVLTIAG
                     SETTRNAISAGLMALLEHPDQLELLRSDPDVMSSAVEEILRWTAPAAYFRRTATVDGE
                     IRGVPIAAGDRVTIWHPSANRDEDAFEDPFRFDITRADNPHVTFGGGGVHYCLGAKLA
                     KREIRVMFEELLARVRDFEVVGEPEYSVQGIGNPITVSLKDLPVRLTAA"
BASE COUNT          533 a         1137 c         1086 g          511 t
ORIGIN      
        1 cggcgccgcg cacaccgcag ggatcgcgtt gagcaagcgc atcgcggtgg cgagcaaacc
       61 gccggtgttg tggtcgccgt cctcgccgag catcgacagc tcgcaccgga acgacggcga
      121 gcccttcacc tcgatgcggt acccaccacc ttcgggcggc gcgggccact ccggcgcgag
      181 gtcgtcacgc aagcgcgtca cgtgctcgac cgtgagcacc ggctcaccgt cgacgatccc
      241 ttcgatctcg aaccgcagtc ccgcgacggt ccccgcctcg accgttcccg ccgcgatctc
      301 gaacgtctcc ggcgcgggca cgcggtcgta gcgctcgcgg atctcgtcga cttccacacc
      361 gaggccttcg gccatgaggt ggatcaccga accccacgcg tacccgagca ctcccggcat
      421 caagaggaac ggcacctgat ccatcggttg cccgaagccc atcgtgtcga acaagacggt
      481 cgcctggtcg tacgtgtcgt agttgaggat ctccgcgaca cggatcgaat cgacgcgcgc
      541 gcagaagccg ctgaacgtca acggcagcaa gtcgttcgcg aagccggggt cgattcccga
      601 ggagaagcac gatgtctgcc ccttcgcgca cgcggcctcg agctggtcga ccatcccgcg
      661 atcggcggtg cccggatgca acaggctgat caccgcgctc gacaccacgt tcttccccga
      721 ctcgagcaac gcggcgatct cggccgcggc ttccgcggga cgcagatcgc cggtggagtt
      781 gtgtgacact gcgtcggcct cgagggccag gatctcctcg aagtcgttcg tcgtgagcac
      841 acccgtcgac gggcgtccgc agagctcgcc cgcgtccttc cccgccttgt cggcactgtg
      901 cacccacaag cccacgagct cgaggtcggg atgctccagg attccctgga gcgcgagccg
      961 ccccacgttg cccgtcgcga actggatcac cttgaaggac atgtccgaaa tctctcacac
     1021 tggcgcggtg accgtgctgc gccgcctcgg cgccgtcctg gccgtggtgg cggtcgcgct
     1081 gctcactgcg gcgacgacgg cggcgtccgc ggcggccaag gacccgttcg ccgcgctgga
     1141 ccggtcgatg cgcgaccgcg tgaaggacga cgatctcgcg ggcgcggtgc tcctcgtggt
     1201 gcgcgacgac gccgtgatcc atgcgacgga gttcggcgcg ctcgacgccg attcggtgat
     1261 cccgatcgcg tccgcgagca agtggctcac ctcggccacg ctcatgacgc tggtcgacga
     1321 cggcaaggtc tcgctcgacg aatcgatcgc gacgtatctc ccagagttca agggctccaa
     1381 caagaagaag gcgatcaccg tccgccagct gctggcgcac acccacggtc tcccgtacgc
     1441 gagctgcatc ggcgatccga cgaccacgac cgcgaattgc accgcggcca tcgcgaacgg
     1501 cccggacccg gtcacgagac cgggcaaggt gttctcgtac ggcggcgtgg gctacgaggt
     1561 cgcagcacgc atcatcgagg tgatcagcgg ccagtcgttc gaggacgcct tcgagacgcg
     1621 catcgcgcgg ccgctgggca tgacgcacac cagcttcgac gagtaccagg gccagcgctt
     1681 ccgccatcca cagccgtcgg gatcagcggt gtcgtcggtc gacgagtacg cgaagttcct
     1741 cgccatgctc accgcgggcg gcgagagtgc gaacgggcgc gtgctcaccg aggactccgt
     1801 cgccgagatc gaacgtgacc aggtgcgtgg catcgacacc catcgtgacg cggcggtgca
     1861 gatcacgaac atcccgacgt acggactcgg tgtgtggcgc gacgtcgcga gcaagaccga
     1921 cgacatcgaa gtggtgagtg gcagcggcgc gtacgggttc tatccgtgga tcgaccgccg
     1981 caacggcaac tacggcatcg tcggcgtggc cgacctcacg aacggcgccg aacacgcggt
     2041 acccgcgtcg caacgacagg cgcgcgtcgc gtggcgcgcc gccgcgcact gggtgccgta
     2101 gacgagtcca gcggttcagg cggcggtgag ccgcaccggc agatccttca agctcacggt
     2161 gatcgggttg ccgattccct gcacgctgta ctccggttcc ccgacgacct cgaagtcgcg
     2221 cacacgagcg agcagctctt cgaacatcac cctgatctct cgcttcgcga gtttggcgcc
     2281 gaggcagtag tgcacaccgc cgccgccgaa ggtgacgtgc gggttgtcgg cgcgcgtgat
     2341 gtcgaatcgg aacgggtcct cgaaggcgtc ttcgtcgcgg ttcgccgacg ggtgccagat
     2401 cgtgacgcgg tcgccggccg cgatcggcac gccgcggatc tcgccgtcaa cggtcgcggt
     2461 gcgccggaag tacgccgccg gcgcggtcca gcgcaggatc tcttcgaccg cgctgctcat
     2521 cacgtcggga tcactgcgga gcagctcgag ctggtcgggg tgctcgagca gtgccatgag
     2581 gccggccgag atcgcgttgc gcgtggtctc gctgcccgcg atcgtcagca cgatgaagaa
     2641 caggtcgagc tcgaccggag agaggcgagt gcgctcaccg tcgtcgtgct cgatctccgc
     2701 ctggatgagc gtggtccaga cgtcgtcggc cggtacgcgg cgcttctcct cgcccagctg
     2761 ctgcgcatag ccgaacatct cggcctggat cgcgttgatc tcgtcgcggc tgagccgtga
     2821 ccgcggatcg gtggacaaca ggaagacgtt cacacggtcg aacagccatt cgcgatcgga
     2881 caccgggatg ccgacgatgt cggcgatcat gtgcatcggg agctggtacg ccacttcgtg
     2941 cacgaagttg cactgcttct gctcgagcgc gcgatcgacg atcgcactgg cccacgcccg
     3001 cgcctggtcc tcgagcttcg tgatcatgcg cggcgtgaac cccgcgctca cgagccgtcg
     3061 catgcgcgtg tgatcgggcg ggtccattga caccaccatc gccccgcgtt gctccggtgg
     3121 aagggtcggc agcgacgggc cgtcgagtga gctgaaccgc gcgggatcgc ggctcacggc
     3181 ctgcacgtcg gcgtgcttcg agaccaccca gaacgacccg ccgagccgat cggtgccccg
     3241 gtgctctggg tgccaccaca ccggcgc
//
LOCUS       DASUGA010000603         2023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_476285, whole genome shotgun sequence.
ACCESSION   DASUGA010000603 DASUGA010000000
VERSION     DASUGA010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476285"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VFX21_11955"
     CDS             <1..891
                     /locus_tag="VFX21_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5096726.1"
                     /translation="VLGIVGESGSGKSVTMLAVLGLLPRTAKVTGSVKFRGAELVGIK
                     KKEIQRIRGAKISMIFQDPLTALNPVIRVGDQIVEAIRAHHRDISKSAAMEKAVSLLD
                     DVGIPRPEVRVRQYPHEYSGGMRQRAMIAMMIANDPDVIIADEPTTALDVTIQAQILD
                     VLKSIQERTGTAVVFITHDLGVIARVADRVQVMYAGRTAELASVDDLFDDSRHPYTRG
                     LLASLPALNARSERLQPIIGSPPSMLNPPSGCAFHPRCPLARPVCTEEQPPLRDVGAE
                     GQKAACYFSEELPSHSKAMA"
     gene            888..1934
                     /locus_tag="VFX21_11960"
     CDS             888..1934
                     /locus_tag="VFX21_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019806497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX5096727.1"
                     /translation="MTEVADAPPADRVEGTPILEVTNLVKEFPVRGTRGKFVVNAVSD
                     VSFELFPGKTLGLVGESGSGKSTVGRCILRLLEPTAGSVTFDGADMLRLNSKEMRRYR
                     KRVQIVFQDPYASLDPRMTVAAIIEEPMKIHRTPGKHKDTVKELLSLVGLNPEHGNRY
                     PHEFSGGQRQRVGIARAIALEPDMLVLDEPVSALDVSIQAGVVNLLEDLQDRLGLAYL
                     FIAHDLSVVRHISDDLAVMYLGKIVEKGPAEQVYMRPQHPYTQALLSAVPVPEPGLER
                     GRTRIMLEGDVPSPVHPPSGCRFRTRCWKATDLCAQEEPELIDRGAGHPVACHYAADA
                     TVDVAITTDDAASS"
BASE COUNT          349 a          680 c          656 g          338 t
ORIGIN      
        1 gtgctcggca tcgttggcga gtcggggtcg ggcaagtcgg tgacgatgct tgccgtgctc
       61 gggctgctgc cgcggaccgc caaggtgacg ggatcggtca agttccgcgg cgccgagctg
      121 gtcggcatca agaagaagga aatccaacgc attcgcggcg ccaagatctc gatgatcttc
      181 caggacccgc tcaccgcgct caatccggtg atccgggtcg gtgaccaaat cgtcgaggcg
      241 atccgcgcgc accaccgcga tatctcgaag tcggcggcga tggagaaagc ggtctcgctg
      301 ctcgacgacg tcggcattcc ccgccccgaa gtgcgtgtgc gtcagtaccc gcacgaatac
      361 tccggcggca tgcgccagcg cgcaatgatc gcgatgatga ttgccaacga cccggacgtg
      421 atcatcgccg acgagccgac caccgcgctc gacgtcacga ttcaggcgca gatcctcgac
      481 gtgttgaagt cgatccagga gcgcaccggc acggccgtgg tgttcatcac gcacgacttg
      541 ggcgtgatcg ctcgcgtcgc cgaccgggtg caggtcatgt acgcaggtcg cactgcggag
      601 ctcgcgtcag tcgacgacct ctttgacgat tcccggcacc cgtacacgcg tgggctgttg
      661 gcgtcgttgc cggcgctcaa tgcgcgctcc gaacgcttgc agccgatcat cggatcgccg
      721 ccctcgatgc tgaacccgcc gtcgggatgc gccttccatc cccgctgccc gttggcgcgt
      781 ccggtgtgca cggaggaaca gccgccgctg cgcgacgtgg gcgccgaagg ccaaaaggcc
      841 gcgtgctact tctccgaaga gttgccctcg cacagcaagg cgatggcatg acggaagtgg
      901 cagacgcgcc gcccgccgac cgggtcgagg gaacgccgat cctcgaggtc acgaacctcg
      961 tgaaggagtt tccggtccgc ggcacgcgtg gcaagttcgt cgtgaacgca gtttccgacg
     1021 tttcgttcga gctgttcccc ggcaagacgc tcggcttggt gggggagtcc ggttccggaa
     1081 aatcgaccgt cgggcgctgc attcttcggc tgctcgaacc gacggccgga tcggtgacgt
     1141 tcgacggcgc cgacatgctt cggttgaact cgaaagagat gcgccgctac cgcaagcggg
     1201 tgcagatcgt gttccaggac ccgtacgcat cgctcgaccc gcgcatgacg gtcgccgcga
     1261 tcatcgaaga gccgatgaag attcaccgga cgcccggcaa gcacaaggac accgtgaagg
     1321 aacttctttc gctcgtcggg ctgaaccccg agcacggcaa ccgctacccg cacgagtttt
     1381 cgggcggaca acgccaacgg gtcggtatcg cgcgcgcgat cgcgctcgag cccgacatgc
     1441 tggtgctgga cgagcccgtc tccgctctcg atgtttcgat ccaagccggt gtcgtcaacc
     1501 tgctcgaaga tctgcaggac cggctcgggc tcgcgtacct cttcatcgcg cacgacttgt
     1561 cggtcgtgcg ccacatctcc gacgacctcg cggtcatgta tctcggcaag atcgtcgaga
     1621 aggggccggc cgagcaggtg tacatgcggc cgcagcaccc ctacacgcag gcgttgctct
     1681 cggccgtgcc ggtacccgag cccggtctcg aacggggccg cacgcgcatc atgctcgaag
     1741 gcgacgtgcc gagcccggtg cacccgccga gcggctgccg cttccgcacg cggtgctgga
     1801 aggcaaccga cctctgcgcg caggaggaac cggagctcat cgaccgcggc gccggccatc
     1861 ccgtggcgtg ccactacgcc gccgacgcga cggtcgacgt ggcgatcacc accgacgacg
     1921 ccgccagctc gtaacctcgg atcgaacctg cgtgagatgt gcgcgtggcg cactccattc
     1981 acgcaggttc gcaattgcta ggatcttcgc ttccacgtcg gag
//
LOCUS       DASUGA010000604         2425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_216354, whole genome shotgun sequence.
ACCESSION   DASUGA010000604 DASUGA010000000
VERSION     DASUGA010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216354"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VFX21_11965"
     CDS             complement(<1..310)
                     /locus_tag="VFX21_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096728.1"
                     /translation="MTEPGAGNRKFQVLAVEPDERFRTRMTIELAGITPAPLTDIDDV
                     LHNLAPGEPTVVLCGPGYASETGLGQIQRISRSFPEVGLVLMAEDLTLPLLQSALRAG
                     V"
     gene            complement(335..1222)
                     /gene="cpaB"
                     /locus_tag="VFX21_11970"
     CDS             complement(335..1222)
                     /gene="cpaB"
                     /locus_tag="VFX21_11970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX5096729.1"
                     /translation="MRNWRVLTAIAAVVLAAIAGVLVWQYVNDADDRAESDLDQIEVL
                     VATKTIQRGTSGETAIDNGLIETQKRVEKDVPDNALRPDQEKDIKELFAAGTIAKGRE
                     LFVEDFIAGGQVQNTSLEIADGKQAISISLDDSHGVAGFVVPGDTINLIVTADIKNGA
                     DPKPEAQDITVKTTAFLLPGVKVLGVGSTTTFGQTSRKVDTNGDGVVDENDAEPETRQ
                     LQQGLWTLEVTPRQAEQIAHAQASSMPVYATLNPASFDLGKWVYPDEIVEVANFFDQP
                     LPKVEEVLRALSAAKKSAG"
     gene            complement(1559..>2425)
                     /locus_tag="VFX21_11975"
     CDS             complement(1559..>2425)
                     /locus_tag="VFX21_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX5096730.1"
                     /translation="LPASLVEMRRVRDLQPYVGKEIEAKIIELDKNRNNVVLSRRAWL
                     EQTQSEVRSEFLHQLQKGQVRKGFVSSIVNFGAFVDLGGVDGLVHVSELSWKHIDHPS
                     EVVEVGTEVTVEVLDVDLDRERVSLSLKATQQDPWQEFANGHQVNELVYGRVTKLVPF
                     GAFVQVGEGIEGLVHISEMAAHHVEAPEQVVTPGEELWVKIIDIDLERRRISLSIKQA
                     AEGGEVAAEYREFYGEHAYDEQGNYIGPDYHAEGEAPAEGEATEPAAPDADAVVAADA
                     PATEATPDAPTA"
BASE COUNT          359 a          832 c          799 g          435 t
ORIGIN      
        1 gcactcccgc ccgcaacgcg gactggagca gcggcaacgt caggtcttcg gccatcagca
       61 cgagcccgac ttcggggaac gagcgcgaga tgcgctggat ctggcccaac cccgtctcgc
      121 tcgcgtaccc cgggccgcac agcacgaccg tcggctcgcc cggcgcgagg ttgtgcagca
      181 cgtcgtcgat gtcggtgagc ggcgccggcg tgatgccggc gagctcgatc gtcatgcgcg
      241 tccggaagcg ctcgtcgggt tcgaccgcga gtacctggaa cttgcggttg cccgcgcccg
      301 gttcggtcat ctgctccgct ccgatcacgg tgcgctaccc ggcgctcttc ttcgccgcgc
      361 tcagggcccg cagcacttcc tcgaccttgg gcagtggctg gtcgaagaag ttggcgacct
      421 cgacgatctc gtcggggtac acccacttgc cgagatcgaa cgatgccggg ttcagcgtcg
      481 catagacggg catcgacgac gcttgcgcgt gcgcaatctg ctcggcctgg cgaggcgtga
      541 cttcaagcgt ccacagaccc tgctgcagct ggcgcgtttc gggctcggcg tcgttctcgt
      601 cgacgacgcc gtcgccgttg gtgtcgacct tgcgcgacgt ctgcccgaac gtcgtggtcg
      661 aacccacgcc gagcaccttc acacccggca gcaggaacgc cgtcgtcttg acggtgatgt
      721 cttgggcctc cggcttcggg tccgccccgt tcttgatgtc cgcggtgacg atcaagttga
      781 tcgtgtcgcc cgggacgacg aagccggcga cgccgtgcga gtcatcgagg ctgatcgaga
      841 tcgcctgctt gccgtcggcg atctcgagcg aggtgttctg cacctgaccg ccggcgatga
      901 agtcctcgac gaagagctcg cggcccttgg cgatcgtgcc cgccgcgaag agctccttga
      961 tgtccttctc ctggtcggga cggagtgcgt tgtcgggaac gtccttctcg acgcgctttt
     1021 gcgtctcgat caggccgttg tcgatcgcgg tctcgcccga cgtgccgcgc tggatggtct
     1081 tggtcgccac cagcacctcg atctggtcga gatcgctctc ggcgcggtcg tcggcatcgt
     1141 tcacgtactg ccagaccagc acgccggcga tcgcggccaa gacgaccgcc gcgatcgcgg
     1201 tcagcacccg ccagttgcgc accccacgcc cccttgctcg caccggtgcc cccggtgcgc
     1261 tcggttccta cggccgtcgc ctccctgcga caaccggccg cggaagaggc acgaatgcgg
     1321 ctacgtgcga cttctgcgga gctatcgggt acaaggtaca aacactgtcc gtcgaacacc
     1381 agtaggccat ttggccccgc accgcgcgat ccgtggcgga atgtggcgcg agcgtgaacg
     1441 caatgggcga ctcggtgcac atccggaaca atgccgcgga actggtacat ctgtccacac
     1501 gcgacgacgc ccgctcacga tcgagcgggc gtcgaaatgt gaaattcgcg acagcgcgtc
     1561 aggcagtcgg agcgtccggt gttgcctccg tcgccggcgc gtcggccgcg acgaccgcgt
     1621 cggcgtcggg cgcggcgggt tccgtcgctt caccttcggc cggggcctcg ccctcggcgt
     1681 ggtagtcggg gccgatgtag ttgccctgct cgtcgtacgc gtgctcaccg tagaactcgc
     1741 ggtactcggc cgcgacctcg ccaccttcgg cggcctgctt gatcgacagc gagatccgcc
     1801 gccgttcgag gtcgatgtcg atgatcttca cccacagctc ttcgccgggg gtgacgacct
     1861 gctcgggcgc ctcgacgtgg tgcgccgcca tctcggagat gtgcaccagg ccctcgatgc
     1921 cttcgcccac ctggacgaac gcaccgaacg gcaccagctt ggtgacccgg ccgtacacga
     1981 gctcgttgac ctggtggccg ttcgcgaact cctgccacgg gtcttgctgg gtcgccttga
     2041 gcgacaggga gacgcgctcg cggtccaggt cgacgtcgag aacctcgacg gtgacctcgg
     2101 tgccgacctc gacaacctcg gacgggtggt cgatgtgctt ccaggacagc tcggagacgt
     2161 gcaccaggcc gtcgacaccg ccgagatcga cgaacgcgcc gaagttgacg atggaggaca
     2221 cgaagccctt gcggacctgg cccttctgga gctggtgcag gaactcgctg cggacctcgg
     2281 actgggtctg ctcgagccat gcgcggcgcg acagaacgac gttgttgcgg ttcttgtcga
     2341 gctcgatgat cttggcctcg atctccttgc cgacgtaggg ctgcaggtca cggacccgcc
     2401 gcatctcgac gagcgaggcc ggcag
//
LOCUS       DASUGA010000605         5085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_649357, whole genome shotgun sequence.
ACCESSION   DASUGA010000605 DASUGA010000000
VERSION     DASUGA010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649357"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VFX21_11980"
     CDS             <1..362
                     /locus_tag="VFX21_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096731.1"
                     /translation="TGAPATTAPVATTAPGPNQPPVIAFNGRTIDMGDDRIPSWDHFW
                     IYTSVKDPENDQWTFNSVRADSTEDIYTGSCAEDTTHGCIWYWDNGLCGVDSIWVTVK
                     DSHGNVSNEDVLKADYC"
     gene            454..1407
                     /locus_tag="VFX21_11985"
     CDS             454..1407
                     /locus_tag="VFX21_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX5096732.1"
                     /translation="MAQTDPHGRGSAEPSDILARGWKDVLVRVKREAKDDNLSLLSGG
                     VAFFAMLSLVPALIAVVSIYGLVADPSDVERQAREYTRALPDEARDLVIQQLRNVVNS
                     SSGGLGLAAIGGIFLALWSASSAVKHLITAVNTIYEEDEGRGFVKVRGLSLLLAVGAA
                     VFIVGAILLIAVLPSALGDSSLGTAARWIFNVLRWPLLAVGMIFALAVVYRVGPDRDD
                     PKWRWISWGAVFATIAWIIASVLFSIYTSNFGSYNETYGSLGAVIVLMLWLYLSALLV
                     LLGAELNSEIEHQTARDTTVGPERPMGARRARMADEVGAAR"
     gene            complement(1404..2042)
                     /locus_tag="VFX21_11990"
     CDS             complement(1404..2042)
                     /locus_tag="VFX21_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5096733.1"
                     /translation="MAEKQAAPTKPKPRRYDSSRRSRQAEQTRREVLMAALDLFNERG
                     WSGTTLAAIAERAGVAVETIYSGFGSKKGLLRATVDVGVVGDTEPVPFAEREEAARMT
                     RGSREARLRAATEIQTDIHERLAGVWQAVVAAASSDEEVDGWRKELDDGRRIDTRRFL
                     ALIYEREIDEHVVDLIWALFSSEVYWKLTRDVGWSRSEYETWLYTAMERVVD"
     gene            2133..2954
                     /locus_tag="VFX21_11995"
     CDS             2133..2954
                     /locus_tag="VFX21_11995"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5096734.1"
                     /translation="MDLPVLTPDPYRVASDTWLIPTFADAPGLGYHAAHSLVIRGREP
                     IVVDTGTSFVRDAWLHNAFSVVDATDVRWIFISHDDHDHIGNIDAVVELCPNATIVAN
                     FGVVGRLALDVALPLERMRWLEPGDVLDAGDRTLTLVRPPLFDSPSTRGFHDSATGLL
                     WAVDSFGALFPGAAFEADDIPGDLYDESFTLLNMLNTPWMEWVDRDRFAAHVRAVSSL
                     PIDVVTSAHGPVLRGAQIGDAFRRTLALAGATPPPSPGQPLLDLLAGSLAAPAAI"
     gene            2967..3452
                     /locus_tag="VFX21_12000"
     CDS             2967..3452
                     /locus_tag="VFX21_12000"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5096735.1"
                     /translation="MGTLPRTGRNEVVVDAPVAAVWDVVVDVTRTGEWSHECQRVEWL
                     DGAHVAKVGARFVGRNRAGRFSWSRRSEIVRLEPGREIVWKTIPTLVYRDSTEWSLLI
                     EPAGDSRTRIVQSYQVLMINPIANRLFYLMVPQHRDRRPELNHDLLRIGEVANATTAA
                     R"
     gene            complement(3468..>5085)
                     /locus_tag="VFX21_12005"
     CDS             complement(3468..>5085)
                     /locus_tag="VFX21_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013424424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5096736.1"
                     /translation="VLGRADDDGVALLFEDEQWTWREVVAEARTRARLFLELRQPGPF
                     HVGVLLENVPEFVFVLTGAGLAGAAVVGINPTRRGAELARDINHTDCQLIVTDASMRP
                     LLDGLDLDVPADRIFDVDGPEYAALVAKARDDTRVIEPPAPETLFALLFTSGSTGAPK
                     AVRMTQGRAARGAANVIFSPDDVLYCAMPLYHGNALNANLFPALRSGAAIALRQKFSA
                     SGFLPDARRYNVTFFNTVGRALNHILSTPESADDRDHSIKYVLGPESAPADVRAFKKR
                     FGVPVFEGYGSSENAIMMYPDPSAPKGSLGVPMEGVEVVILDPVTAEEKPRARYDEHG
                     KLLNASEAIGEIVSKNATARFEGYYNNPEADAERTRNGWYWSGDLGYRDEGGVFWFAG
                     RNADWIRVDGENFAAAPIERILARFPGADGIAVYAVPDSRTGDQVMAAIEMLDGATFD
                     PVAFARFLDEQRDLGTKWAPRYVRVVDELPVGATNKLDKRPLRAARWETTDPVYWRPP
                     RAAEYVPFTATDAATLQREFEANDRTNLLT"
BASE COUNT          773 a         1747 c         1709 g          856 t
ORIGIN      
        1 cgaccggcgc gccggccacg accgcaccgg tcgcgacgac ggcgcccggc ccgaatcagc
       61 cacccgtcat cgcgttcaac ggccgcacga tcgacatggg cgacgaccgc attccgagtt
      121 gggaccactt ctggatctac acgtcggtca aggatccgga gaacgatcag tggacgttca
      181 acagtgtccg ggccgacagc accgaagaca tctacaccgg ctcctgcgcc gaggacacga
      241 cgcacggatg catctggtac tgggacaacg ggttgtgcgg cgtcgactcg atctgggtca
      301 ccgtcaagga ctcccacggg aacgtgtcga acgaggacgt gctcaaagcc gactactgct
      361 gagcgcctcg ccggaccacg gcgaacgtgg ccgcgagcgg tcggtcggtc ctcccgagtt
      421 ttcggagccg ctcggggcgg gcatccttcg ctcgtggcgc agaccgatcc gcacgggcgt
      481 ggctccgccg aaccctccga catcctggcg cggggatgga aggacgtcct ggttcgggtc
      541 aagcgcgaag cgaaggacga caacctgtcg ttgctctccg gcggcgtcgc attcttcgca
      601 atgctgtcgc tggtgcccgc cttgatcgcc gtcgtctcca tctacgggct cgtcgcggat
      661 ccgtccgacg tcgagcgtca ggcgcgcgag tacaccagag cgctgccgga cgaggcgcgc
      721 gacctcgtca tccagcagct gcgcaatgtc gtgaactcgt cgagtggcgg tctcggtctc
      781 gcggcgatcg gcggcatctt cctcgcgctc tggagcgcgt cgtccgcggt caagcacctc
      841 atcacggccg tcaacacgat ctacgaagag gacgaaggtc gcggattcgt caaagtgcgc
      901 ggcctgagtc tgctgctcgc agtcggcgcc gcggtcttca tcgtcggcgc gatcctgttg
      961 atcgcggtgc tgccgtcggc gctcggcgat tcgagcctcg ggaccgcggc gcgctggatc
     1021 ttcaacgtcc tgcgttggcc tttgctcgcg gtcgggatga tcttcgcgct ggccgtcgtg
     1081 taccgcgtgg ggcccgatcg tgacgatccg aagtggcggt ggatctcgtg gggcgccgtc
     1141 ttcgcaacga tcgcatggat catcgcgtcg gtcttgttct cgatctacac gtccaacttc
     1201 ggctcgtaca acgagaccta cggctcactg ggggcggtca tcgtgttgat gctgtggctc
     1261 tatctcagcg cgctgctggt gctcctggga gccgagctga actcggagat cgagcatcag
     1321 accgcgcgcg acaccaccgt cgggcccgaa cggccgatgg gcgcgcgccg ggcgcgcatg
     1381 gccgacgaag tcggcgccgc gcgctagtcg acgacgcgtt ccattgcggt gtagagccac
     1441 gtctcgtatt cggaccggct ccaaccaacg tcgcgcgtga gcttccagta cacctcggac
     1501 gagaacagcg cccagatgag atcgacgaca tgctcgtcga tctcgcgttc gtagatcagc
     1561 gcaaggaagc ggcgcgtgtc gatgcgacgg ccgtcgtcca gctccttgcg ccagccgtcg
     1621 acctcttcgt cggatgacgc agccgcgaca actgcctgcc acacaccggc cagccgctca
     1681 tggatatcgg tctggatctc ggtcgcggcg cgcagccgcg cctcccgtga accgcgggtc
     1741 atgcgcgcgg cttcttcgcg ctcggcgaag ggaacgggtt cggtgtcgcc gacgaccccg
     1801 acgtccaccg tggctcgcag caggcccttc ttggatccga agccgctgta gatcgtctcg
     1861 accgcgacgc cggcccgttc cgcgatcgcg gcgagggtcg tgcccgacca gccccgttcg
     1921 ttgaacaggt cgagcgcagc catgagcacc tcgcggcggg tctgctccgc ctgccggctg
     1981 cggcgcgaag agtcgtagcg gcgcggcttc ggcttggtcg gcgcggcttg cttctcggcc
     2041 atgggttgca ttctagagcc tgctctgatt caatacaatc tagttccact gaatccagtc
     2101 acgttccaac tgaatcccga ggagggaccg ccatggacct gcccgtgctc acccccgacc
     2161 cctaccgagt cgcgtccgac acgtggctga tcccgacgtt cgcggatgcc ccggggctcg
     2221 gttaccacgc ggcccacagc ctcgtgatcc gcggccgaga gccgatcgtc gtcgacaccg
     2281 gcacctcgtt cgtgcgcgac gcgtggctcc acaacgcgtt ctcggtcgtc gatgcgaccg
     2341 acgtgcgttg gatcttcatc tcgcacgacg accacgacca catcggcaac atcgacgcgg
     2401 tcgtcgagct gtgcccgaac gcgacgatcg tcgcgaactt cggtgtcgtc ggtcggctcg
     2461 cgctcgacgt cgcgctgccg ctcgagcgca tgcgctggct cgaaccgggg gacgtgctcg
     2521 acgccggcga ccgcacgctc acgctcgtgc ggccgccact gttcgactcg ccgtcgacgc
     2581 gcggcttcca cgactccgcg acgggcctgc tgtgggcggt cgactcgttc ggcgcgctgt
     2641 tccccggcgc ggccttcgag gcggacgaca ttcccgggga cctgtacgac gagagcttca
     2701 cgctgctcaa catgttgaac acgccgtgga tggaatgggt cgatcgtgac cgattcgccg
     2761 cgcacgtacg cgcggtgtcg tcgctgccca tcgacgtcgt cacgagcgca cacggtccgg
     2821 tgttgcgcgg cgcgcagatc ggcgacgcgt tccgccgcac gctcgcgctc gcaggtgcaa
     2881 cgccgccgcc ctcgccggga caacctctgc tcgatctgct cgccggttcg ctcgccgcgc
     2941 ccgcggcgat ctgaaaggat ttcgtcatgg gaacgctgcc gcgcaccggc cgcaacgagg
     3001 tggtcgtcga tgcgccggtc gcagccgtgt gggatgtcgt cgtcgacgtc acgcgcacgg
     3061 gcgaatggag ccacgagtgc caacgcgtcg agtggctcga cggcgcgcac gtcgccaagg
     3121 tgggcgcccg tttcgtcggc cgcaaccgcg ccgggcggtt cagctggtca cgacgtagcg
     3181 agatcgtgcg cctcgaaccg gggcgcgaga tcgtgtggaa gacgattccg acgctcgtgt
     3241 accgcgacag caccgaatgg tcgctgctga tcgaaccggc cggtgacagc cggacgcgca
     3301 tcgtgcagag ctaccaggtg ctcatgatca acccgatcgc gaaccggctc ttctatctga
     3361 tggtgcccca gcatcgcgac cgtcggcccg agctcaacca cgacctgctc cgcatcggcg
     3421 aagtcgcgaa cgcgacgact gcggcgcgtt gaagcacgag ccacgcgtta cgtcaggaga
     3481 ttcgtgcggt cgttcgcttc gaactcgcgc tgcagcgtgg cggcgtcggt ggcagtgaac
     3541 gggacgtact cggcggcccg tggtggccgc cagtacaccg gatcggtggt ctcccaccgc
     3601 gccgcgcgca gtggacgctt gtcgagcttg ttggtcgcgc cgacgggcag ctcgtcgacc
     3661 acgcggacgt accgcggcgc ccacttcgtg ccgagatcgc gctgctcgtc gaggaagcgc
     3721 gcgaacgcga ccgggtcgaa cgtcgcgcca tcgagcatct cgatcgcggc catcacctgg
     3781 tcaccggtgc ggctgtcggg gaccgcgtac accgcgatgc cgtcggcgcc gggaaagcgg
     3841 gcgaggatgc gttcgatcgg cgcggccgcg aagttctcgc cgtcgacgcg gatccagtcc
     3901 gcgttgcggc ccgcgaacca gaacacgccg ccctcgtcgc gataaccgag atcacccgac
     3961 caataccagc cgttgcgcgt gcgctcggcg tcggcctccg ggttgttgta gtagccctcg
     4021 aagcgcgcgg tggcgttctt gctgacgatc tcgccgatcg cctcgctcgc gttcagtagc
     4081 ttcccgtgct cgtcgtagcg ggcccggggc ttctcttcgg cggtcaccgg gtcgaggatc
     4141 acgacctcga cgccttccat cggcacgccg agcgatccct tcggcgcgct ggggtcggga
     4201 tacatcatga tcgcgttctc gctcgatccg tagccctcga acacgggcac accgaagcgc
     4261 ttcttgaacg cgcgcacgtc ggcgggtgcc gactccggac cgagcacgta cttgatcgag
     4321 tggtcgcggt cgtccgcgct ctcgggcgtc gagaggatgt ggttcagcgc gcgaccgacg
     4381 gtgttgaaga acgtgacgtt gtaccggcgc gcatccggca agaagcccga cgcggagaac
     4441 ttctgccgga gcgcgatggc ggcacccgat cgcagcgcgg ggaagagatt cgcgttgagc
     4501 gcgttgccgt ggtacagcgg catcgcgcaa tagagcacgt cgtcggggct gaagatgaca
     4561 ttggcggcgc cgcgcgcagc gcgaccttgg gtcatgcgca ctgccttggg cgcgcccgtc
     4621 gaccccgacg tgaacagcag cgcgaacagg gtctcgggcg cgggcggttc gatcacgcgg
     4681 gtgtcgtcgc gcgccttggc gaccagcgcc gcgtactccg gaccgtcgac gtcgaagatg
     4741 cggtcggccg gcacgtcgag atcgaggccg tccagcagcg gccgcatcga ggcgtcggtg
     4801 acgatcagct ggcagtcggt gtggttgatg tcgcgcgcca gctccgcgcc gcgtcgcgtc
     4861 gggttgatgc cgaccacggc cgctcccgcg agcccggcgc ccgtgagcac gaacacgaac
     4921 tcgggcacgt tctcgagcag cacgccgacg tgaaacggac ccggttgccg cagttcgagg
     4981 aacagacgcg cgcgcgtgcg cgcttcggcc acgacctcgc gccatgtcca ctgctcgtcc
     5041 tcgaacagca gcgcgacccc atcgtcgtcg gcccggccga gcacc
//
LOCUS       DASUGA010000606         4078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2057551, whole genome shotgun sequence.
ACCESSION   DASUGA010000606 DASUGA010000000
VERSION     DASUGA010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2057551"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1700
                     /locus_tag="VFX21_12010"
     CDS             <1..1700
                     /locus_tag="VFX21_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HEX5096737.1"
                     /translation="WLLGMYLALADEQGVPYEKLTGTTQNDVLKEYLSRGTYIFPPEA
                     SKRLTVDLVVYTVKNVPKWNPINVCPYHLQEAGASPVQELAYGLANAIAVLDAVRESG
                     QVPADEFPQVVGRISFFIDAGVRFVEEMCKMRAFTRLWDQICEQRYGVTDAKLRRFRY
                     GMQVNSLGLTEEQPENNVQRIVLEMLGVTLSKDARARAIQLPCWNEALGLPTPWDQQW
                     ALRMQQVLAFESDLLEYGDLFDGSKVVEAKVAELCEAAAAELQWVLDGGGSYTMIDDM
                     KGRLVQSHAERVRRIEAGEQKVVGVNTFTETEPSPLAAIAQRGGIVTIDPEVERAQIA
                     ALDQWRAARDAAAVEAALAELRRVAATTENLVPATVALARAGGTVGEWAGALREVFGE
                     YRAPTGVGAVAVRPGPEMEVVRERVKAALGHHGPLRILVGKPGLDGHSNGAEQIAVAA
                     RDAGMEVVYQGIRLTPAQIAAAARDEDVDVVGLSILSGSHRELVPETVRLLREAGVDA
                     PVVVGGIIPDTDRAQLEAAGVARIYTPKDYRLAAIMEDIADLALEHRTAQAEATTGR"
     gene            1709..2497
                     /locus_tag="VFX21_12015"
     CDS             1709..2497
                     /locus_tag="VFX21_12015"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5096738.1"
                     /translation="MQEPRLVLAVGAGVAALAAGVIGAATDSAVLGIVAGVAGVGAAM
                     AGVALGARARQSDDRLAQAEDEIRRVKRELASINAVLQEEASRRAAEAELGAPGDMLP
                     EKGEHAFDAATGLYDERYFAVLVQQQVAAARRSLRPISVVIFEIDGMGNSDQETQAQA
                     LGVVGDVVRRTLRESDAACRLGDLMVGAILEDTPEAGAVWAAERVRGTLLSSPIGDAI
                     TLSAGVACYPTHALGAAELVQQAGRALDEARSRGRDRVELAPDA"
     gene            complement(2507..3634)
                     /locus_tag="VFX21_12020"
     CDS             complement(2507..3634)
                     /locus_tag="VFX21_12020"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5096739.1"
                     /translation="MQALDGLKVIDMATVFAGPGAARHLADFGADVVKVESPAGDGVR
                     RMGWFPPEGGDSYTWKLLGRNKRTVVIDLKADGGRDALLALADGADVLIENFRPGTLE
                     RLGLGPDVLHARNPRLVILRVTGFGQDGPYAQRPGFATMAEAMSGFAAINGEPDGGPL
                     LPPIALTDEVSALAGAFAVMVALRHRDATGEGQVVDVSLLESMVQLMSALPSAAAHLD
                     YDQPRLGSGIPYSVPRGTYQCADGAWVAVSTSAESIAHRVLTLLGVEGDERFATFASR
                     AEHREELDALLRAWVAARNEDEVLAAFKAAEAAIAPVYTMRQLLVDPHAKARGIFIEV
                     DGIVQQGPAARLSKTPAEIRHAGRPLGTDSLSIPPAESGSD"
     gene            complement(3641..>4078)
                     /locus_tag="VFX21_12025"
     CDS             complement(3641..>4078)
                     /locus_tag="VFX21_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096740.1"
                     /translation="WLDDVTASSTKPVLVFGHHHPWDPSSHERNPHYFGINPDDSETL
                     CALIDRRESIAGYFAGHTHRTRKREFAQARNVPIVEIACVKDYPGAWAEYQVYEGGYI
                     QLTRRIATPDALDWTEKTRNMFAGLYRDYALGRLADRCFTRAV"
BASE COUNT          624 a         1399 c         1461 g          594 t
ORIGIN      
        1 tgtggttgct cggcatgtac ctcgcgctcg ccgacgaaca gggcgtgccc tacgagaagt
       61 tgacgggcac gacccagaac gacgtgctga aggagtactt gtcgcgcggc acgtacatct
      121 tcccgcccga ggcgtcgaag cgactcaccg tcgacctcgt ggtctacacc gtgaagaacg
      181 ttccgaagtg gaacccgatc aacgtctgcc cgtaccactt gcaagaggcg ggtgcgtcac
      241 cggtccagga gctcgcgtac ggcttggcga acgcgatcgc agtgctcgac gccgtgcgcg
      301 agtcggggca ggtgcccgcc gacgagttcc cgcaagtggt cggccgaatc tcgttcttca
      361 tcgacgccgg cgtgcggttc gtcgaagaga tgtgcaagat gcgcgcgttc acgcgcctgt
      421 gggaccagat ctgcgaacag cgctacggcg tcaccgacgc gaagctgcgc cgcttccgtt
      481 acggcatgca ggtcaactcg ctcggcctca ccgaagagca gcccgagaac aacgtgcagc
      541 gcatcgtgct cgagatgctc ggcgtgacgc tgtcgaagga cgcgcgggcg cgtgcgatcc
      601 agttgccgtg ctggaacgag gcgttggggt tgccgacccc gtgggaccag cagtgggcgt
      661 tgcgcatgca gcaggtgctc gcgttcgagt cggacttgtt ggagtacggc gacttgttcg
      721 acggctccaa ggtcgtcgag gcgaaggtcg ccgagctgtg cgaagccgcg gccgcggagt
      781 tgcagtgggt gctcgacggc ggcggctcgt acacgatgat cgacgacatg aaggggcggc
      841 tcgtgcagag ccacgccgag cgcgtccgcc ggatcgaagc gggcgagcag aaggtcgtcg
      901 gcgtgaacac tttcaccgag accgagccgt cgcccctcgc cgccatcgcg caacgcggcg
      961 gcatcgtcac catcgacccc gaagtcgaac gcgcgcagat cgccgcgctg gaccagtggc
     1021 gcgcggcgcg tgacgcggcc gcggtcgagg ccgcgctggc ggagctgcga cgggtcgcgg
     1081 cgacgaccga gaacttggtc ccggccactg tcgcgctggc gcgcgcgggc ggcaccgtcg
     1141 gcgagtgggc cggcgcactg cgcgaggtgt tcggcgagta ccgcgcgccc accggagtcg
     1201 gcgcggtggc cgtccgcccg gggccggaga tggaagtggt gcgggagcgc gtgaaggccg
     1261 cgctcggcca ccacgggccg ctgcggatcc tcgtcggcaa gcccggtctc gacggccact
     1321 ccaacggcgc ggagcagatc gcggtggccg cccgggacgc cgggatggag gtcgtctacc
     1381 aggggatccg gctcaccccg gcccagatcg cggcggccgc ccgggacgag gacgtcgacg
     1441 tcgtcggcct ctccatcctc tccgggtcgc accgggagct ggtccccgag acggtccgct
     1501 tgctgcgcga ggccggcgtg gacgcgccgg tcgtggtcgg cgggatcatt ccggacacgg
     1561 atcgcgcgca actcgaggcc gcgggcgtag cccggatcta cacgcccaag gactaccgtc
     1621 tcgcggccat catggaagac atcgccgatc tggcgctcga gcaccgcact gctcaggcgg
     1681 aggcaaccac cggccgatga gacaccgcat gcaagaaccc cgtttggtgc tcgccgtcgg
     1741 cgcgggcgtt gccgcgctcg ccgccggagt catcggtgcc gctaccgact cggccgtgct
     1801 gggcatcgtc gcgggcgtcg ccggtgtcgg tgcggcgatg gccggcgtcg cgctcggtgc
     1861 gcgcgcccgc cagagcgacg accggctggc tcaggccgaa gacgagatcc gccgcgtgaa
     1921 gcgcgagctg gcgtcgatca acgccgtgct gcaggaagaa gcgagccgcc gcgccgccga
     1981 agccgagctc ggcgcgcccg gcgacatgct ccccgagaag ggcgagcacg cattcgacgc
     2041 ggcgaccggt ctgtacgacg agcgctactt cgccgtcttg gtgcaacagc aggtcgcggc
     2101 cgcccgccgg tcgctgcgcc cgatctcggt cgtcatcttc gagatcgacg gcatgggcaa
     2161 ctccgaccag gagacgcagg cgcaagccct cggcgtcgtc ggcgacgtcg tccgccgcac
     2221 cctgcgcgag agcgacgcgg cctgccgcct cggcgacctc atggtcggcg cgatcctcga
     2281 ggacaccccc gaagccggcg ccgtctgggc cgccgaacgg gtgcgcggca cgttgctgtc
     2341 gagcccgatc ggcgacgcga tcacgctgtc cgccggtgtc gcctgctacc ccacccacgc
     2401 cctcggcgcc gcggagttgg tccaacaagc cggccgcgcg ctcgacgagg cccgcagccg
     2461 cggccgcgac cgggtagagc tcgcccccga cgcgtaacga tctttgtcaa tctgacccgc
     2521 tctcggcggg cgggatcgac aaagaatcgg taccgagcgg ccggccggcg tgtctgatct
     2581 ccgcgggggt cttcgacaag cgcgccgcgg ggccttgttg gacgatgccg tcgacctcga
     2641 tgaagatgcc gcgggccttg gcgtgcgggt ccaccaacag ctgacgcatc gtgtagacgg
     2701 gcgcgatcgc ggcttcggcg gccttgaacg cggcgagcac ctcgtcttcg ttgcgggctg
     2761 cgacccacgc tcgcaacaac gcgtccagct cctcgcgatg ctcggcccgg ctggcgaacg
     2821 tggcgaagcg ttcgtcgcct tcgacgccga gcaacgtgag cacgcggtgc gcgatcgact
     2881 ccgccgacgt cgacaccgcg acccaagcgc cgtccgcgca ctggtaggtg ccgcgcggca
     2941 ccgagtacgg aatgccggag ccgagccggg gctggtcgta gtcgaggtgc gcggccgccg
     3001 acggcagcgc cgacatcagt tgcaccatcg attcgagcag gctgacgtcg acgacttggc
     3061 cctcgccggt cgcgtcgcga tggcgcagcg cgaccatgac cgcgaatgcg cccgcgagcg
     3121 cgctgacttc gtcggtcaac gcaatcggcg ggaggagcgg tcccccgtcg ggttcgccgt
     3181 tgatggcggc gaacccgctc atcgcttcgg ccatcgtcgc aaagcccggc cgttgcgcgt
     3241 agggaccgtc ctgaccgaag ccggtgacgc gcaggatcac gaggcgcggg ttgcgcgcgt
     3301 gcagcacgtc gggtccgaga ccgagccgtt cgagcgtgcc cggacggaag ttctcgatca
     3361 acacgtcggc gccgtcggcg agcgcgagca acgcgtcgcg accgccgtcg gccttcaggt
     3421 cgatgacgac agttcgcttg ttccggccga gcagcttcca cgtgtacgag tcgccaccct
     3481 ccggcgggaa ccagcccatg cggcggactc cgtcaccggc gggcgactcg accttcacga
     3541 cgtccgcgcc gaagtcggca agatgccgcg ccgcgccggg cccggcaaac accgtcgcca
     3601 tgtcgatcac cttcagcccg tcgagcgcct gcatcggcgg ctacacagcg cgggtgaagc
     3661 agcggtcggc caatcggccg agtgcgtagt cgcggtagag acccgcgaac atgttgcgcg
     3721 tcttctcggt ccagtcgagc gcgtcgggtg ttgcgatgcg gcgcgtgagt tggatgtaac
     3781 cgccctcgta cacctggtac tcggcccacg caccgggata gtccttcacg cacgcaatct
     3841 cgacgatggg gacgttgcgc gcctgcgcga actcgcgctt gcgggtgcgg tgcgtgtgcc
     3901 cggcgaagta gccggcaatc gactcgcggc gatcgatcaa cgcgcacagc gtctcgctgt
     3961 cgtcggggtt gatgccgaag tagtgcgggt tgcgctcgtg tgagctcggg tcccagggat
     4021 gatggtgacc gaacacgagc accggcttcg tcgaagacgc ggtgacgtcg tcgagcca
//
LOCUS       DASUGA010000607         2371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_198533, whole genome shotgun sequence.
ACCESSION   DASUGA010000607 DASUGA010000000
VERSION     DASUGA010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198533"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /locus_tag="VFX21_12030"
     CDS             complement(<1..1287)
                     /locus_tag="VFX21_12030"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX5096741.1"
                     /translation="MTLTYKLGIDVGGTFTDCVLLAPDGSLTLEKSPTTPHDQSEGVL
                     AGIERLATAVGCASARELLARTQSIVHGTTTGDNTMIQMAGAPTGLLVTEGFRDEIEF
                     RRCYKEDIWDPTLPAPEPIARRRVRLEIRERLTAEGDVDTPLDEDGVRRAARRLRAFG
                     VRSVAVTFLHSYIDPRHELRARELILEEFPEVELISLSHEVYPKPPEFERTSTTLVNA
                     YVGPPIVRYLDRLQQRLRENGYENELLIATSSGGISTPASIGQRAVATIGSGPTGGVV
                     AAARAATAAGLGDVVSVDMGGTSYDVCLIRDGRPELKAEWNWRHRYCIALPMVDIHAI
                     GAGGGSIARVVGGTLHVGPESAGAVPGPVCYGRGGTEPTVTDANVVLGRLDPDAFWGG
                     RLRLDVAAARDSLARLGKELGLDAEETAAAVVTIVDA"
     gene            complement(1284..>2371)
                     /locus_tag="VFX21_12035"
     CDS             complement(1284..>2371)
                     /locus_tag="VFX21_12035"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX5096742.1"
                     /translation="QRFSDEISQWPDGTYEADMYVDSDPAGNRDIHVHVAITIAGDRM
                     IVDFAGSDERPEINGWSTFGNTRGYTISQLASLVDPTIPKNEGFFECIDLRVPEGTCL
                     NPTVGKPVSAGTHHPGVEVGDAIAVAMSQILPDRCAPQTYKYGAPRQMWGDCDPRTAR
                     PFFDHGGETNAGWVNAVRGVDGWGALVASNGNLIKASAELNEVLFPHILRGRNYRTDS
                     GGAGEWRGAPGSHFVKEVRTPTYVNQYVVNQYHVHPGIAGGANGAPDRCVLHAGTDHE
                     VVVDPTVAGELLATGEWLLYDFGGGGGWGDPLARDPQAVLDDVWDEYVSIDAARREYG
                     VVITGSLADMTLAIDEAATAAERATRG"
BASE COUNT          348 a          837 c          826 g          360 t
ORIGIN      
        1 cgcgtcgacg atcgtgacga ccgcggccgc ggtctcctcg gcgtcgagac cgagctcctt
       61 gccgaggcgc gccaacgagt cgcgcgccgc cgccacgtcg agccgcaggc ggccacccca
      121 gaacgcgtcg ggatcgaggc ggccgagcac gacgttcgcg tcggtcaccg tcggttcggt
      181 cccgccccgg ccgtaacaga cggggcccgg caccgcgccg gccgactccg gcccgacgtg
      241 cagcgtgccg ccgacgacgc gggcgatcga gccgccgccc gcgccgatcg cgtggatgtc
      301 gaccatgggc agcgcgatgc agtaccgatg cctccagttc cattccgcct tgagctcggg
      361 ccggccgtca cggatgaggc acacgtcgta cgaggtaccg cccatgtcga cgctgaccac
      421 gtcgccgaga ccggcggccg tcgccgcgcg cgccgcggca accacaccac ccgtggggcc
      481 cgagccgatc gtcgcgaccg cgcgctgccc gatcgacgcc ggcgtcgaaa tgccgcccga
      541 cgacgttgcg atcagcaact cgttctcgta accgttctcc cggagccgct gttggagccg
      601 gtcgaggtag cggacgatcg gcggaccgac gtacgcgttg acaagcgtcg tcgacgtccg
      661 ctcgaactcc ggaggcttcg gatagacctc gtggctcagc gagatgagct cgacctcggg
      721 aaactcctcg agaatcagct cgcgagcgcg cagctcgtgg cgcggatcga tgtaggagtg
      781 gaggaacgtc accgcgaccg acctcacgcc gaaggcccga agccgtcgcg cggctcggcg
      841 cacaccgtcc tcgtcgaggg gcgtgtcgac gtcgccctcg gccgtcaggc gttcgcggat
      901 ctcgagccgc acccgccggc gcgcgatcgg ctcgggggcg ggcagggtcg ggtcccagat
      961 gtcttccttg tagcaacggc ggaactcgat ctcgtcgcga aagccctcgg tgacgagcag
     1021 acccgtcggc gcgcccgcca tctggatcat cgtgttgtca ccggtggtgg tcccgtgcac
     1081 gatcgactgc gtccgggcga ggagttcacg cgcactcgca caccccaccg cggtcgccaa
     1141 ccgctcgatg cccgcgagca caccctcgga ttggtcgtgc ggcgtggtcg gcgacttctc
     1201 gagcgtcaac gaaccgtcgg gtgccagcaa cacgcagtcg gtgaaggtgc cgccgacatc
     1261 gatacccaac ttgtacgtga gggtcaccct cgcgtcgccc gttcggcggc agttgcggct
     1321 tcgtcgatcg ccagggtcat gtcggcgagg gaaccggtga tgacgacgcc gtactcgcgc
     1381 cgggccgcgt cgatcgagac gtactcgtcc cacacgtcgt cgagcacggc ctgcggatcg
     1441 cgggccagcg gatcgcccca gccgccgccc ccaccgaagt cgtacaggag ccactcgccg
     1501 gtcgcgagca actcgccggc caccgtcggg tcgacgacca cctcgtggtc ggtgcccgcg
     1561 tgcagcacgc aacgatcggg cgcgccgttc gcgccgcccg cgatgccggg gtgcacgtgg
     1621 tactggttga cgacgtactg gttgacgtag gtcggcgtgc ggacttcctt cacgaagtgc
     1681 gaacccggcg cgccgcgcca ctcgccggcg ccgcctgaat cggtgcggta gttgcggccc
     1741 cgcaggatgt gggggaacag cacctcgttg agctccgccg atgccttgat cagattgccg
     1801 ttcgacgcga cgagcgcgcc ccagccgtcg acgccgcgca ccgcgttgac ccaccccgcg
     1861 ttcgtctcgc cgccgtggtc gaagaacggg cgagccgtgc gcggatcgca gtcgccccac
     1921 atctggcgcg gcgcgccgta cttgtacgtc tgcggggcgc agcggtcggg caggatctgc
     1981 gacatcgcga ccgcgatcgc gtcgccgacc tcgacgccag gatggtgcgt gcccgcggag
     2041 accggcttgc cgacggtcgg gttgaggcac gtcccctcgg gcacgcgcag gtcgatgcac
     2101 tcgaagaagc cttcgttctt cgggatcgtc gggtcgacga gcgacgcgag ctgggagatc
     2161 gtgtagccgc gtgtgttgcc gaacgtcgac cagccgttga tctcgggacg ttcgtcggag
     2221 ccggcgaagt cgacgatcat gcggtcgccg gcgatggtga tcgcgacgtg cacgtggatg
     2281 tcgcggttgc cggccgggtc gctgtcgacg tacatgtcgg cttcgtacgt gccgtcgggc
     2341 cactgcgaga tctcgtcgga gaatcgttgg c
//
LOCUS       DASUGA010000608         2598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_58357, whole genome shotgun sequence.
ACCESSION   DASUGA010000608 DASUGA010000000
VERSION     DASUGA010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58357"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VFX21_12040"
     CDS             complement(<1..181)
                     /locus_tag="VFX21_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012379563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HEX5096743.1"
                     /translation="MTYTGVVPEAYQQVPYMPGPRVLTRVPDGWDDRTYERITLRPLA
                     PTIGGEVGGADLARLD"
     gene            complement(244..1062)
                     /locus_tag="VFX21_12045"
     CDS             complement(244..1062)
                     /locus_tag="VFX21_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX5096744.1"
                     /translation="MRTTFAVLAACAAMCVGAVATSANAAPTWAPAATAAIHPGVQTL
                     TEGAQCTANFIFSDGTNVYIGQAAHCAGTGGNTETNGCTAGSLPLGTQVEVGGATRPG
                     TIVYSSWLQMQTDHETDESTCAYNDIALVRLDPADVSRVNPSVPFWGGPTGLDTDDTQ
                     FGENVYSYGNSSLRQGITLLSPKRGISLGSTDGWTHNVYTVSPGIPGDSGSAFVNANG
                     QALGVLSTLAVAPLPASNGVSDLASMLDYMRAHTNLDAIQLVDGTEPFSANRLL"
     gene            1150..>2598
                     /locus_tag="VFX21_12050"
     CDS             1150..>2598
                     /locus_tag="VFX21_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096745.1"
                     /translation="MNRRLVGLVLLAVAPMALSACKRRDVSTVTRPYDAVVVTGASIP
                     AVQGIAPNRLVAFRFVDGTWQQIPVQVDERAVLDLATPKHLAPIGKTATFYTDANTWT
                     GADPNADIDADDEISFMARDAHGQARDIDKDGAYVLTRPAHTASNGVEIEVNDPRKTD
                     SAAWVYLFQSDGTLDPAAGAPSINYSYALNAGDYKTKYSISVGPNPENSRVTTNWYSL
                     HFSDRWITDEMKITATGATGADILDRHKSGFAGSCARTEDTFSAGPGAMIANKTGPVR
                     AIRSVLGANSGTYTQRDHIMYESRVDVVTYLRVHAIPPLRDWLDYSPAASGMTYRTNI
                     NPSGVTIDGVPDALPTDEVQYQLVSGAPGSVVHTSRLDTSIPINPGDIKTFYRDDLTP
                     PETQCTGDAVEYGASGWQFDKDVPCTDPALNCTDYLTTTRRMTFLGPSATTNEGNTIA
                     AETATPLTTTVKAFALNEVRASYKGELRPCKTP"
BASE COUNT          474 a          897 c          839 g          388 t
ORIGIN      
        1 cgtcgagtcg cgccagatcg gcgccgccga cttcgccgcc gatcgtcggc gcgagcggcc
       61 gcagcgtgat gcgttcgtag gtgcggtcgt cccagccgtc gggcacacgc gtgagcacac
      121 gcggtccggg catgtacggg acctgctggt acgcctcggg aacgacgccg gtgtaggtca
      181 tgcgcgcgcc tcctcattct aatggattac attagaacga ggagggcgct cgcgcaaggg
      241 ggctcagagg agacggttgg cgctgaacgg ctcggtgccg tcgaccagct ggatcgcgtc
      301 gaggttcgtg tgggcccgca tgtaatcgag catcgacgcc aggtcgctga cgccgttcga
      361 cgcgggcagc ggggcgaccg ccagggtgct gagcacgccg agcgcctggc cgttggcgtt
      421 gacgaacgcg ctgcccgagt cacccgggat gcccggcgac acggtgtaga cgttgtgagt
      481 ccagccgtcc gtcgagccga ggctgattcc ccgcttgggt gagagcaacg tgatgccctg
      541 gcgcaacgac gagttgccat aggagtacac gttctcgccg aattgcgtgt cgtcggtgtc
      601 gagccccgta ggaccacccc agaacgggac cgacgggttc acacgcgaga cgtcggcggg
      661 atcgagacgc accaatgcga tgtcgttgta cgcgcacgtc gactcgtcag tctcgtggtc
      721 ggtctgcatc tgcaaccacg agctgtagac gatcgtgccc ggacgggtcg cgccgccgac
      781 ctcgacctgc gtgccgagcg gcaacgaacc cgcggtgcaa ccgttcgtct cggtgttccc
      841 gccggtaccc gcgcagtgcg cggcctgacc gatgtacacg ttcgtcccat cggagaagat
      901 gaagttggcg gtgcattgcg caccttcggt gagcgtttgt acaccgggat gaatcgcggc
      961 agtggcagcc ggcgcccacg ttggcgccgc gttggccgag gtcgcaacgg ctccgacaca
     1021 catcgccgcg cacgcggcga gaactgcgaa ggttgtacgc acgaatacct ccacgcgagg
     1081 aaaggatcac tgaaatggat atccggccgt gagccgcgcg cggacgggac ttctcgttac
     1141 cctctcgcca tgaatcgacg cctcgtggga ctggttttgc tcgcggtcgc gcccatggcc
     1201 ctctcggcgt gcaagcggcg cgacgtctcg accgtcaccc gcccgtacga cgcggtcgtg
     1261 gtcaccggcg cgtcgatccc ggccgtgcaa ggcatcgccc cgaaccggct cgtcgccttc
     1321 cgcttcgtcg acggcacgtg gcagcagatc ccggtgcagg tcgacgaacg tgccgtcctc
     1381 gacctcgcga caccgaagca cttggccccg atcggcaaga cggcgacgtt ctacaccgac
     1441 gccaacacgt ggaccggcgc cgaccccaac gcggacatcg acgccgacga cgagatctcg
     1501 ttcatggcgc gtgacgcgca cggccaagcc cgcgacatcg acaaggacgg cgcctacgtg
     1561 ctcacgcggc ccgcgcacac cgcgtcgaac ggcgtcgaga tcgaagtcaa cgacccccgc
     1621 aagaccgaca gtgcggcctg ggtctatctg ttccagagcg acggcacgct cgacccggcg
     1681 gccggtgcgc cgtcgatcaa ctacagctat gcactcaacg ccggtgacta caagacgaag
     1741 tacagcatca gcgtcgggcc gaacccggag aactcgcgcg tcacgacgaa ctggtactcg
     1801 ctgcacttct ccgaccgttg gatcaccgac gagatgaaga tcaccgccac cggcgccacc
     1861 ggcgccgaca tcctcgaccg gcacaaatcc ggattcgcgg gaagctgcgc gcgcaccgag
     1921 gacacgttct cggccggccc gggcgcgatg atcgccaaca agaccgggcc cgtgcgcgcg
     1981 atccggtcgg tgctgggtgc gaacagcggc acgtacacac agcgcgacca catcatgtac
     2041 gagagccgcg tcgacgtcgt cacgtacttg cgcgtgcacg cgatcccgcc gctgcgcgac
     2101 tggttggact actcgcccgc ggcgtccggc atgacatacc gcaccaacat caacccgtcg
     2161 ggcgtgacga tcgacggcgt acccgacgcg ttgccgaccg atgaggtgca gtaccaactc
     2221 gtcagcggcg cgcccggttc ggtcgtgcac acgtcgcgac tcgacacgag catcccgatc
     2281 aaccccggcg acatcaagac gttctaccgc gacgacctca caccgccgga gacgcagtgc
     2341 accggcgatg ccgtcgagta cggcgcgtcg ggctggcagt tcgacaagga cgtgccgtgc
     2401 accgatcccg cgttgaactg cacggactac ctgaccacga cccgccgcat gacgttcctc
     2461 ggcccgtcgg cgacgacgaa cgagggcaac acgatcgcgg ccgaaaccgc gacgccactc
     2521 acaaccaccg tcaaggcgtt cgcccttaac gaggtacggg cgtcttacaa gggcgaatta
     2581 cgcccatgta agacgccc
//
LOCUS       DASUGA010000609         2412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_58448, whole genome shotgun sequence.
ACCESSION   DASUGA010000609 DASUGA010000000
VERSION     DASUGA010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58448"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /locus_tag="VFX21_12055"
     CDS             complement(<1..1269)
                     /locus_tag="VFX21_12055"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5096746.1"
                     /translation="MRARPNLLLFMPDQLRADAVGAFGNPVVQTPNIDTLADSGIQFA
                     SAYSQHSVCAQSRISMFTGWYPHVAGHRSLEYLLAEHEPNIFRRLRGGGYHVALAGAR
                     GDMMAPGITRASSDRFGFATAPNLNDLARWHASPFDPGSKWYDAFYGGSVDEELFEFD
                     AATVATAVDWLHEGLPEPWCLFVPLVFPHPPFTVERQWYSRYEDVDVPAPVPPTFEGK
                     PGFYREIHTRYGLDRLEAEDWDEIIRTYYAMVTRVDHQLGQVLAAVDRAGCAERTVTF
                     FFTDHGEYLGDFGLVEKWPSGVDDVLVRNPLIVHVPDGRSGTAQTFVEMIDLTATLDE
                     LAALDPVPHFGRSLVPVLDDPGTVHRDAAFSEGGFLLAEEPLLEDGDEGQYRHKQAIH
                     HERPELVGRAVAIRTDQWCYVERLYEGPELY"
     gene            complement(1273..2097)
                     /locus_tag="VFX21_12060"
     CDS             complement(1273..2097)
                     /locus_tag="VFX21_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013426172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX5096747.1"
                     /translation="MDSGFIDYEAAGGIATITLNRPEAANAQNMPLLDDLDEAWRTAA
                     DDDDVRVIILQANGKHFSAGHDLKGVSNGEVPAKITLSYIYEAEARRFLEYSLRWRNV
                     PKPSIAAVQGVCIAGGLLLCWPCDLIIAADNASFSDPVVNMGIGGVEYHGHTWEWGAR
                     KAKELLFTGRAMSAHEAEQVGMVNKVVPLDDLRSETRALAEVIASKHPFALRQAKRAV
                     NQTLDVQGFYAAIQAVFDVHQTGHGHALSESGLPILMTLEGMKQEVAKPAPSGKPT"
     gene            2222..>2412
                     /locus_tag="VFX21_12065"
     CDS             2222..>2412
                     /locus_tag="VFX21_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096748.1"
                     /translation="MAEPQTDAQLDEFMKVRKPGAHLEEDVVADDFVLMLGVDLVNYP
                     KFPKADDTVTYAKADLDRR"
BASE COUNT          396 a          790 c          819 g          407 t
ORIGIN      
        1 gtagagctcg ggcccttcgt agagccgctc gacgtaacac cattggtcgg tgcggatcgc
       61 cacggcgcgc ccgacgagct ccggtcgttc gtgatggatc gcctgcttgt gccggtactg
      121 gccctcgtcg ccgtcctcga gcaacggctc ctcggcaagg aggaagccgc cttcgctgaa
      181 cgccgcgtcg cgatggaccg tgccgggatc gtcgagcacg ggcacgagtg accgaccgaa
      241 gtgcggcact ggatcgagcg cagccagttc gtcgagcgtc gcggtgaggt cgatcatctc
      301 gacgaaggtc tgggccgtgc ccgagcggcc gtcgggaacg tgcacgatca gcgggttccg
      361 cacgagcacg tcgtctacgc ctgacggcca cttctccacg agtccgaagt cgccgaggta
      421 ctcaccgtga tcggtgaaga agaaggtgac cgtccgctcg gcgcagccgg cgcggtcgac
      481 ggccgcgagc acctgaccga gctgatggtc gacgcgggtg accatcgcat agtaggtgcg
      541 gatgatctcg tcccagtcct cggcttcgag acggtcgagc ccgtagcgcg tgtggatctc
      601 gcggtagaag cccggcttgc cctcgaaggt cggcggcacc ggcgcgggca cgtcgacatc
      661 ctcgtagcgc gagtaccact gtcgttcgac cgtgaacggc gggtgcggga agacgagcgg
      721 tacgaagaga caccacggct cgggtagccc ttcgtgcaac cagtcgacgg cggtggccac
      781 cgtcgccgcg tcgaactcga acagctcctc gtcgacggat ccgccgtaga aggcgtcgta
      841 ccacttcgat ccgggatcga atggggaggc gtgccatcga gcgaggtcgt tcaggttcgg
      901 cgcggtcgcg aacccgaagc gatcggatga cgcgcgcgtg atacccggcg ccatcatgtc
      961 gccacgtgca ccggccaacg caacgtggta gccgccgcca cgtaagcgcc ggaagatgtt
     1021 tggctcgtgc tcggccagca ggtactcgag tgaccggtgg ccggccacgt gcggatacca
     1081 gcccgtgaac atcgagatcc ggctctgcgc gcacacgctg tgctgcgagt acgcgctcgc
     1141 gaactggatg cccgaatcgg ccagcgtgtc gatgttcggt gtctgcacga ccgggttgcc
     1201 gaacgcgccg accgcgtcgg cgcgcagctg gtcgggcatg aacagcagca ggttggggcg
     1261 ggcgcgcacg cgtcaggtgg gcttgccgct cggtgccggc ttggcgactt cttgcttcat
     1321 gccttcgagc gtcatgagga tcgggagccc cgactcgctg agcgcgtggc cgtgtccggt
     1381 ctggtggacg tcgaagaccg cttggatcgc ggcgtagaac ccttgcacgt cgagcgtctg
     1441 gttcacggcg cgcttcgctt ggcgcagggc gaacggatgc ttgctggcga tcacctcggc
     1501 cagcgcgcgc gtctcgctcc ggagatcgtc gagtgggacg accttgttca ccatgccgac
     1561 ctgctcggct tcgtgcgcgc tcatcgcccg cccggtgaac aagagctcct tggccttgcg
     1621 cgcgccccac tcccacgtgt gcccgtggta ctcgacgcca ccgatgccca tgttcacgac
     1681 cggatcggag aacgaggcgt tgtcggcggc gatgatgagg tcgcacggcc agcacaacag
     1741 cagccccccg gcaatgcaca caccctgcac cgccgcgatc gacggcttgg ggacgttgcg
     1801 ccatcgcaac gagtactcga ggaaccgccg cgcctcggcc tcgtagatgt acgacagcgt
     1861 gatcttcgcc gggacctcgc cattgctcac gcccttcagg tcgtggcccg cggagaagtg
     1921 cttgccgttg gcttggagga tgatcacgcg gacgtcgtcg tcgtcggccg ctgtacgcca
     1981 tgcctcgtcg agatcgtcga gcagcggcat gttctgcgcg ttggctgcct cggggcggtt
     2041 gagcgtgatg gtggcgatgc cgccggccgc ctcgtagtcg atgaatcctg agtccacggc
     2101 gaccccctcg cggaaatggc gttctccgat gacgtgcacc ggaatctacc gcgagacccg
     2161 gaactagccg atcggctagt ctgccctcgt tcgcctggat cgcggaacga gggggagccc
     2221 gatggccgaa ccgcagaccg atgcgcagct cgacgagttc atgaaggtgc gcaagcccgg
     2281 cgcccacctc gaagaagacg tggtcgccga cgacttcgtg ttgatgctcg gcgttgacct
     2341 cgtcaactac cccaagttcc cgaaggccga cgacaccgtc acctacgcca aagcggatct
     2401 ggacagacgc ca
//
LOCUS       DASUGA010000610         2514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1864643, whole genome shotgun sequence.
ACCESSION   DASUGA010000610 DASUGA010000000
VERSION     DASUGA010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1864643"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VFX21_12070"
     CDS             complement(<1..473)
                     /locus_tag="VFX21_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096749.1"
                     /translation="MFDPSEGLDDLERAIDKLTPERPLDLPRLRRLADRLAAHFVHAV
                     GAAERAGRLGGPNTGAASALTDACRMPYAEASRLVEMAHAFEDLPVTSVAVLDGEISL
                     DHARVMTRACTKARVDAFRDVEGQLVDMAKEWDVRPFAKRVKRLCDAVDGDDGVTA"
     gene            780..1436
                     /locus_tag="VFX21_12075"
     CDS             780..1436
                     /locus_tag="VFX21_12075"
                     /inference="COORDINATES: protein motif:HMM:NF041646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HEX5096750.1"
                     /translation="MQPLHFDIPDLRTIARHVLPRIVEGTLVPVTLLTITLRVLGPKG
                     AMLTGLVWVYGAILTRLVLRRRIPGILLLGAATLSARTAISIASGSTIVYFLQPSLGT
                     VLVASAFLLSVPLGKPLAGRLAADFCPLPDDMSTNEHVRRFFRRISLLWAFAHFSNAT
                     ITIWLLFTQSLGTFVLARVAVSWTVTITAIVASALWFRASMRREGIHVHLPAWRRSTV
                     "
     gene            1415..2185
                     /locus_tag="VFX21_12080"
     CDS             1415..2185
                     /locus_tag="VFX21_12080"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5096751.1"
                     /translation="MASEHSVNAVPGAPLTTFDGLTLASRVLLTDGEPEGAVVIVHGF
                     TASSACPNVEALAARLHDAGLDVVTYDARGHGSSDGESTLGDDEQHDVAAAVEAARRR
                     TDRVVLVGASMGAIAALRYAVDDSQLTGLVCVSCPAEWRLPRNPHGMLAAAMTRTALG
                     RRALARLAGVRVARQWTNPEPPLALAPRLRAPVTFVHGCADRFIGVRDAAQLFEAAPE
                     PRHLMVVPDMGHAFGPTAIDAIERAVAWVFDAAVSERD"
     gene            complement(2169..>2514)
                     /locus_tag="VFX21_12085"
     CDS             complement(2169..>2514)
                     /locus_tag="VFX21_12085"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX5096752.1"
                     /translation="AGRASRALGITPLFEFRDGGAHSLRPALGADAALDRIVARLRRE
                     AIPDARLQVVALHAQAPERAELLLKHVRDAYEPECAFVAPFGVVMQMHTGPGLVGAAW
                     RWIPPDAVSPAR"
BASE COUNT          365 a          912 c          840 g          397 t
ORIGIN      
        1 gcagtgaccc catcgtcacc gtcgaccgca tcgcacagcc gcttcacacg cttcgcgaac
       61 gggcggacgt cccactcctt ggccatgtcg acgagctggc cctcgacatc acgaaacgca
      121 tcgacccgcg ccttcgtgca cgcgcgcgtc atgaccctcg catgatccaa cgagatctcg
      181 ccatcgagca ccgccaccga cgtcaccggc aagtcctcga acgcgtgcgc catctctaca
      241 agacgactgg cttccgcata cggcatccga caggcgtcgg tcagcgcgct cgcggcaccg
      301 gtgttcggcc cgcccagacg accggcgcgt tcggccgcgc ccacagcgtg gacgaagtgt
      361 gcagcgagtc ggtcggcaag cctgcgcaac cggggaagat cgagcggacg ctcgggtgtc
      421 agtttgtcga tcgctcgctc gaggtcgtca agaccctcgg acggatcgaa catgttccgg
      481 ctacactacc cgaacacacg ttcgatatcc agcgattctc gtcataaatc ggtcgccgac
      541 cgacgcgtca cgttgcacgc gacgcgcgcc gttcacgggc gatcgagcgt cggcccaatg
      601 acacatccgc gcgtggcggt cgcctcgggc ggcccggaag gatcgtccgg gcgcgcgtcg
      661 ccggtcgccg gacgctctgt cgattcgtta cccggtgaca gacggggcat gctggcgatc
      721 ctcggcccga gcggcatccg gacctggaag gtccacggcc ggggccgaac aaggaagcaa
      781 tgcagccctt gcacttcgac attcccgacc tgcgcacgat cgcgcgccac gtcctgccgc
      841 gcatcgtgga gggcacgctc gttcccgtca cgctcctgac gatcacgctg cgggtgctcg
      901 ggccgaaggg cgcgatgctc accggactgg tctgggtgta cggcgcgatc ctcactcggc
      961 tggtgctgcg gcgacgcatc cccggcatct tgttgctggg cgcagccaca ctcagcgccc
     1021 gtacggcgat ctcgatcgcg tccgggagca cgatcgtcta cttcctccag ccgtcgctcg
     1081 gcacggtgct cgtcgccagt gcgttcctgc tctcagtgcc gctcggcaag ccgctcgcgg
     1141 gcaggctcgc cgccgacttc tgcccgctgc cggacgacat gtccaccaac gagcatgtcc
     1201 gccgcttctt ccgccgcatc tcgctgctgt gggcgttcgc gcacttcagc aacgcgacca
     1261 tcacgatctg gctgctcttc acccagtcgc tcggcacgtt cgtgctcgca cgggtcgcgg
     1321 tgtcgtggac ggtgacgatc accgcgatcg tcgcatcggc cctttggttc cgcgccagca
     1381 tgcgccgcga agggatccac gtacatttac ctgcgtggcg tcggagcaca gtgtgaacgc
     1441 ggtcccgggg gcgccgctga cgacgttcga cggtctcacg ctcgcgagtc gtgtgctcct
     1501 gaccgacggc gaaccggagg gtgccgttgt catcgtgcac ggcttcaccg cgtcgtcggc
     1561 ctgcccgaac gtggaggcgc tggccgcgcg tcttcacgac gcgggactcg acgtcgtgac
     1621 gtacgacgcg cgcggtcacg ggtcgtcgga tggcgagtcg acgttgggtg acgacgagca
     1681 acacgatgtc gcggccgcgg tcgaggccgc gcgtcgacgc accgatcgcg tcgtcctcgt
     1741 cggtgcgtcg atgggtgcga tcgcggcgct gcgctacgcc gtcgacgact cgcagctcac
     1801 cggactcgtc tgtgtctcgt gcccggccga gtggcgcctc ccgcgcaatc cgcacgggat
     1861 gctcgcggcc gccatgacgc gcacggcgct cgggcgccgg gcgttggcgc ggctggccgg
     1921 cgttcgggtc gcgcgccaat ggaccaatcc cgaaccgccg ctcgcgctcg cgccgcgcct
     1981 gcgcgcgccg gtgacgttcg tgcacggctg cgccgaccgc ttcatcggcg tgcgggacgc
     2041 ggcccaactc ttcgaagccg cgcccgagcc gcggcatctc atggtcgttc ccgacatggg
     2101 ccacgcgttc gggccgaccg cgatcgacgc catcgaacgc gcggtggcat gggtgttcga
     2161 cgcggcggtc agcgagcggg actgaccgcg tcgggcggga tccaccgcca cgcggcaccg
     2221 accagtcccg ggcccgtgtg catttgcatg acgacaccga acggcgcgac gaacgcgcac
     2281 tccggttcgt acgcgtcgcg gacgtgcttc agcaacagct cggcgcgctc gggtgcttgc
     2341 gcgtgcagcg cgacgacctg cagccgagca tcggggatcg cctcacgccg cagccgcgcg
     2401 acgatgcggt cgagcgcggc gtcggcaccg agcgcggggc gcaacgagtg cgcgccaccg
     2461 tcgcggaact cgaacaaggg cgtgataccg agcgcgcgac tcgcccgccc ggcg
//
LOCUS       DASUGA010000611         3412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1032402, whole genome shotgun sequence.
ACCESSION   DASUGA010000611 DASUGA010000000
VERSION     DASUGA010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032402"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VFX21_12090"
     CDS             complement(<1..740)
                     /locus_tag="VFX21_12090"
                     /inference="COORDINATES: protein motif:HMM:NF040244.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HEX5096753.1"
                     /translation="MSNLVDIARQVAEQAHNNEQVEAYVSRATETEVEVFGGEVESLT
                     TASSEGIGIRVIVDQRQGLAWAGSLDADVIAETLKDARDNAAFGEPDEFYGLASPADA
                     AGLTAPELDLWRDELTQVPTDDKVGIALELERATLGLDSRVRVESAGYGDTMSEAALV
                     NSLGIVAQSRRTTVSASSVALADDGSGTQSGYGFAAGRALADLDLEQIPRDAVERSTR
                     LLGAQKIATQRLPVIFDPLVTRSVLGVLT"
     gene            complement(737..2125)
                     /locus_tag="VFX21_12095"
     CDS             complement(737..2125)
                     /locus_tag="VFX21_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX5096754.1"
                     /translation="MIDADVLQSTIDAALRNGGEFAEVFVEDRRSSNGRFDDGRVEEL
                     VSGRSRGAGLRVVRGDTTGFAHTSDLSPDGLRKAAEAASAAARGGGGGTQTVALGDRT
                     TFDLEPETVLPAEVAKQRKVELLAQANDAARAEGSAVTSVTSSYSDARRRILVANSDG
                     LLAEDDRVRTRMMVQAVASGDTGMQTGFEAPGRTMGFELFDEFNPADIGRKAARRALT
                     LLDAVPAPSGRFPVVLKRGAGGVLFHEACGHGLEADHILKDASVFKDRVGESVASPLV
                     TLVDDGGYGREWGTINIDDEGHPARRNVLIENGVLTDYMWDVVRARKEGRAPSGNGRR
                     ETYQHLPMPRMTNTFLLEGATDPDDIIRSVDFGIYCVQLGGGQVEPASGDFVFGVTEA
                     YLIEKGEITRPIRAAQLIGNGPEVLNMVDAVGNDFETWTGMCGKQGQSVPVSSGQPTV
                     LVREMTIGGTAA"
     gene            2231..2617
                     /locus_tag="VFX21_12100"
     CDS             2231..2617
                     /locus_tag="VFX21_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEX5096755.1"
                     /translation="MPIAPGLSASVTLLVEEADTAGYIGSGDVPVLATPRVVALAEQA
                     TMEAVADQLAEGQTTVGYEVQLAHLSPTPIGGKVTAEVTLENVEGRRLSFRLSVTDAR
                     GLVAAGRVTRVIVVRDRFIERAQSEL"
     gene            2614..3288
                     /locus_tag="VFX21_12105"
     CDS             2614..3288
                     /locus_tag="VFX21_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5096756.1"
                     /translation="MSNLLVERHAEHAVLTLNRPEKRNALSVALRDAISDALDEIERD
                     PTMKCVVFTGTGPVFSAGFDLSEFTKGASDDAFMTQLWASSDRYHDRVLRFPLPTIAA
                     INGPALAGGFDLAVCCDLRIATTTAKFAHPERTFGDVVYGPLHDLVGGAVARELAIGG
                     RELRAEDALALHLVGGVVGPEELEAATLALVERVCAAPRDILMRTKAKALRHAGYSGQ
                     APTLEL"
BASE COUNT          536 a         1182 c         1133 g          561 t
ORIGIN      
        1 gtcagtacgc ccagtactga acgagtcacc aacgggtcga agatcaccgg aaggcgctgc
       61 gtcgcgatct tttgggcacc gagcaaacgc gtcgaacgct ccaccgcgtc gcggggaatc
      121 tgttcgagat ccagatcggc gagcgcgcgg ccggctgcga acccatagcc cgactgcgtg
      181 ccgctgccgt cgtcggcgag cgcaacggac gatgccgaca cggtcgtgcg gcggctctgc
      241 gccacgatgc cgagcgagtt caccagcgcg gcctccgaca tcgtgtcgcc gtagccggcc
      301 gactcgaccc gcactcggct gtcgagcccg agggtggccc gctcgagttc gagcgcgatg
      361 cccaccttgt cgtcggtcgg cacctgcgtg agttcgtcac gccacaaatc gagttcgggc
      421 gcggtcagac ctgccgcgtc ggctggtgaa gccagtccgt agaactcatc cggctcaccg
      481 aacgccgcgt tgtcgcgcgc gtccttcagc gtttcggcga tgacgtcggc gtcgagcgaa
      541 cctgcccacg cgagcccttg acgttgatcg acgatgacac gaatgccgat gccctccgac
      601 gacgccgtcg tgagtgactc gacctcgccg ccgaacacct caacctcggt ctcggtcgcc
      661 cgcgagacgt aggcctcgac ttgttcgttg ttgtgcgcct gctccgcaac ctgccgcgcg
      721 atgtcgacga gattgctcat gccgcagtgc cgccaatcgt catctcgcgc acgagcacgg
      781 tgggctgacc cgacgacacg ggcacgcttt ggccctgctt gccgcacatg ccggtccagg
      841 tctcgaagtc gttgccgacc gcgtcgacca tgttcagcac ttcgggaccg ttcccgatga
      901 gctgcgcggc acggatcggc cgcgtgatct cgcccttctc gatgagatag gcctccgtca
      961 cgccgaacac gaagtcgccg ctcgccggtt cgacctggcc accgccgagc tggacgcagt
     1021 agatcccgaa gtcgacgctg cggatgatgt cgtcgggatc ggtcgcgcct tcgagcagga
     1081 acgtgttcgt catgcgcggc atcggcaggt gctggtacgt ctcgcgccgg ccgttgccgc
     1141 tcggtgcgcg accttccttg cgggcccgca cgacgtccca catgtagtcg gtcagcacgc
     1201 cgttctcgat gagcacgttg cgacgagcgg gatgcccttc gtcatcgatg ttgatcgtgc
     1261 cccactcgcg gccatagccc ccgtcgtcga cgagcgtgac gagaggcgac gcgacgcttt
     1321 caccgacgcg gtccttgaac acggacgcgt ccttcagaat gtgatcggcc tcgaggccgt
     1381 gaccgcatgc ctcgtggaac aacacgccgc cggcgccgcg tttcagcacg accgggaagc
     1441 ggcccgaagg cgccggaacc gcgtcgagca gcgtgagcgc gcgccgcgcc gccttgcggc
     1501 cgatgtcggc cgggttgaac tcgtcgaaca gctcgaagcc catcgtgcgg ccgggtgcct
     1561 cgaagccggt ctgcataccc gtgtcgccac tggcgacggc ctgcaccatc atgcgggtgc
     1621 ggactcgatc gtcctcggcg agcaatccgt cggagttcgc caccaagatg cgccggcgcg
     1681 cgtccgaata cgacgatgtg accgacgtga ccgcgctgcc ttccgcccgg gccgcatcgt
     1741 tggcctgggc gagcagctcg accttgcgtt gcttcgcaac ctcggccggc agcacggtct
     1801 ccggttcgag gtcgaacgtc gtgcggtcgc cgagcgcgac cgtctgcgtg ccgccacctc
     1861 cgccgcgcgc cgcggcgctc gcggcttcgg cagctttgcg cagtccgtcg ggcgacaagt
     1921 cggaggtgtg cgcgaacccg gtggtgtcgc cccgaaccac gcgtaaaccg gcgccccggc
     1981 tgcggcccga aacgagctcc tcgacgcggc cgtcgtcgaa ccggccgttc gaggaacgcc
     2041 ggtcttcgac aaaaacctcc gcaaactcgc cgccgttgcg cagcgcggca tcgatcgtgg
     2101 attgcaaaac gtcggcgtcg atcatgatcc tcgacagttc gtgctcaggt tgtggctggg
     2161 gttggcaatg agaaccgcag aaaacgggcc gacatgcccg ttcgccactc gggtgcctat
     2221 cgtaagcggc gtgccgatcg ctcccggtct ctcggcgtct gtgacgctgc tcgtggaaga
     2281 agcagacacc gcgggctaca tcggcagcgg cgacgttccg gtcctcgcaa cgccgcgggt
     2341 cgtggcgctg gccgagcagg ccacgatgga ggccgtcgcc gaccagctgg ccgagggcca
     2401 aacgacggtc ggctacgaag tgcagctcgc acacctctca cccacgccga tcggcgggaa
     2461 ggtcaccgcc gaagtcacgc tcgaaaacgt ggaaggccgc cgcttgtcct tccggctgtc
     2521 ggtcaccgat gcgcgcggct tggttgcggc cggcagagtg acgcgcgtga tcgtcgtacg
     2581 cgaccgattc atcgagcggg cccagtcgga actgtgagca acctgctggt cgagcggcac
     2641 gcggagcacg cggtcctcac gctcaaccgg cccgagaagc gcaacgcttt gtcggtcgcg
     2701 cttcgcgatg ccatcagcga cgcactggac gagatcgaac gcgacccgac gatgaagtgc
     2761 gtcgtgttca ccggcaccgg tccggtgttc agcgccgggt tcgacttgtc ggagttcacc
     2821 aagggcgcat ccgacgatgc cttcatgacc cagctgtggg cgtcgagcga ccgctaccac
     2881 gaccgcgtcc tccggttccc actgccgacg atcgctgcga tcaacggacc cgcgctcgcc
     2941 ggcggattcg acctggcggt ctgctgcgac ctgcgtatcg cgacaaccac cgccaagttc
     3001 gcgcatcccg agcgcacatt cggtgacgtc gtttacgggc cgctccacga cctcgtgggg
     3061 ggtgccgtcg cgcgtgaact cgcgatcggc gggcgcgagc tgcgcgccga ggacgcgttg
     3121 gcgttgcatc tcgtcggcgg cgtggtcgga cccgaggaac tcgaagcggc gacgctcgcc
     3181 ttggtcgagc gggtctgcgc ggcgccgcgc gacatcttga tgcgtaccaa agcgaaggcg
     3241 ctgcgccacg cgggctacag cgggcaggcg ccgacgctgg agctatgacg ttcctcgcaa
     3301 ccgttacgcg aggttgctgc tgcgggggta cgcgtcgacg ggatcggtga gcacgttcac
     3361 cagcgagacc cccttggtcg cgaacgcgcg gtcgagcgcg gcaccgagat cc
//
LOCUS       DASUGA010000612         3357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1256360, whole genome shotgun sequence.
ACCESSION   DASUGA010000612 DASUGA010000000
VERSION     DASUGA010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256360"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFX21_12110"
     CDS             <1..261
                     /locus_tag="VFX21_12110"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX5096757.1"
                     /translation="PVAHVDVYRLVRFQELHDLGLEELLESGRVVVVEWGDVVDQALP
                     AERLVVELGPGGTDDERTVVIDAVGPRWQPRAGALAAAVEAA"
     gene            262..972
                     /gene="tsaB"
                     /locus_tag="VFX21_12115"
     CDS             262..972
                     /gene="tsaB"
                     /locus_tag="VFX21_12115"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX5096758.1"
                     /translation="MLLLGIDTATRRVGVVLANEHGLLGRVEIGGPADTGPPRHAEVL
                     TPAIAYACEQCNVTLAQCSVIAVGIGPGMFTGLRVGVTTAKSLAQSLRIPVIPVPSLD
                     LLAYPLRHTRSLIVPAIDARRHEIYYAIYRPVPGGVQRVSGYELTTPADLVCELEARG
                     VEALLCGDGIARFRDAFRDLERVEFAGPAHSAPSLSALAELAAARYEREEFSAAAEVL
                     PMYLRKSDAELSWDRTKV"
     gene            1137..1697
                     /gene="rimI"
                     /locus_tag="VFX21_12120"
     CDS             1137..1697
                     /gene="rimI"
                     /locus_tag="VFX21_12120"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX5096759.1"
                     /translation="MAAVRKETEALEVHVGPMKRRHLNAVLRIEEQVYPRPWTRSLFV
                     SELALRSTRAYVVARVGRDVVGYAGVMMSITDGHVTTIAVDPKWHRHGIGTRLLLALA
                     REALSRSATALTLEVRLSNTGAQTLYRHFGFTPVGVRKGYYADTGEDALVMWAYEVDR
                     PAYSRLLDSLERQIPGTTVFERTRTS"
     gene            1694..2710
                     /gene="tsaD"
                     /locus_tag="VFX21_12125"
     CDS             1694..2710
                     /gene="tsaD"
                     /locus_tag="VFX21_12125"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX5096760.1"
                     /translation="MTRILAIETSCDETAAAVVDDGVHARSSVVSSQADLHARFGGVV
                     PELASRAHVELIDDVIAEALVEAGSDLGEIDAVAAVHGPGLAGALLVGVSAAKAIALA
                     ADRPYVGVNHHEAHIYAALLEDPTLQPPLVTLIVSGGHTLLVAMRDHGDYEIVGQTVD
                     DAAGEAFDKVARFLGLGYPGGPIIDRLAAQGDPKAVAFPRPMLDDGDGFSFSGLKTAV
                     VQWMRKHPDMEIADVAASFQAAVVDVLVTKLVRVATRLAVPTVVIGGGVAANTALRTR
                     LLDAAETAGLQAVLPSRDLCTDNAAMVGAVAYRRLQADGPTALDGGVYPNLRLGLPPA
                     GAAR"
     gene            2794..3102
                     /gene="groES"
                     /locus_tag="VFX21_12130"
     CDS             2794..3102
                     /gene="groES"
                     /locus_tag="VFX21_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX5096761.1"
                     /translation="MNLQPLEDRIVVRPAEAEETTHSGLVIPDTAKEKPQQGEVLAIG
                     PGRRAEQSGELIPLDIAVGDTVVYSKYGGTEISQDGEDLLILNARDVLAKVAAGKKKK
                     "
     gene            complement(3172..>3357)
                     /locus_tag="VFX21_12135"
     CDS             complement(3172..>3357)
                     /locus_tag="VFX21_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX5096762.1"
                     /translation="VTAVVSFHPGQSATPEEISSTLGGLSRFKQPKHYVFVPVVQRAP
                     NGKADYKWAKETAKRSV"
BASE COUNT          495 a         1147 c         1206 g          509 t
ORIGIN      
        1 ccggtcgcgc acgtcgacgt ctaccggctg gtgcggttcc aggagctgca cgacctcggc
       61 ctcgaagagc tgctcgagag cgggcgcgtc gtcgtggtcg agtggggcga cgtcgtcgat
      121 caggcgctgc ccgccgagcg cctcgtcgtc gagctcggcc cgggcggcac cgacgacgag
      181 cggacggtcg tgatcgacgc ggtcgggccg cgctggcagc ctcgggccgg cgcgctcgcg
      241 gcggcggtgg aggcggcgta ggtgttgctg ctggggatcg acaccgcgac gcggcgggtg
      301 ggcgtcgtgc tcgccaacga gcacggattg ttggggcgcg tcgagatcgg cggtcccgcc
      361 gacaccggac ctccccgtca tgccgaggtg ctgacgcccg cgatcgcgta cgcgtgcgag
      421 cagtgcaacg tgacactcgc gcaatgctcc gtcatcgcgg tcgggatcgg gccgggcatg
      481 ttcaccgggt tgcgcgtcgg tgtgacgacc gcgaagtcgc tcgcgcagtc gttgcgtatc
      541 cccgtcatcc cggtcccgag cctcgacctg ctcgcgtatc cgttgcggca cacgcgcagc
      601 ctgatcgtcc ccgccatcga cgcgcgccgt cacgagatct actacgcgat ctaccggccg
      661 gtgccgggcg gcgtgcagcg cgtctccggc tacgagctga cgacgccggc cgatctcgtg
      721 tgcgagctcg aagcgcgagg cgttgaagcg ttgctctgcg gcgacggcat cgcccggttc
      781 cgagacgcct tccgtgacct cgaacgggtc gaattcgcgg ggcccgcgca ttcggcgccg
      841 agcctgtcgg cgctggccga gctggccgcg gcgcgctacg agcgcgagga gttcagcgcg
      901 gcggccgagg tgctcccgat gtacctgcgc aagagcgacg ccgaactgtc ctgggatcgg
      961 accaaggtat aagtgccccg accggtaacg ttcgccgcgg ccacaccggc cagagacgcc
     1021 gctcgcggcg tcctcctcct cgaaaggggc ccactctgcc ttcgtcgtgc gtccttgcga
     1081 gcgacgcccc tgactcgccg tggccatcga caacgtcacc ggacggagca ctagtcgtgg
     1141 ccgccgttcg caaggagacc gaggcgctcg aggtccacgt cggtccgatg aaacggcggc
     1201 acctcaacgc ggtgctacgc atcgaggaac aggtctaccc gcggccgtgg acgcgttcgc
     1261 tgttcgtcag cgagctcgcg ctgcgatcga cgcgcgcgta cgtcgtggcg cgcgtcggac
     1321 gtgacgtcgt gggctacgcg ggcgtgatga tgtcgatcac cgacgggcac gtgacgacga
     1381 ttgccgtcga tccgaagtgg caccgccacg gcatcgggac gcggctgttg ctcgcgctcg
     1441 cccgcgaggc gctgtcccgt tcggccaccg cgctcacgtt ggaggtccgg ctgtcgaaca
     1501 ccggcgcaca gacgctgtac cggcacttcg gcttcacccc ggtcggcgtg cgcaagggct
     1561 actacgcgga caccggcgaa gacgcgctcg tcatgtgggc gtacgaagtc gaccggcccg
     1621 cgtactcgcg gctgctcgac tcgttggaac gccagatccc gggcacgacc gtcttcgagc
     1681 gaacgaggac atcgtgacgc ggatcctcgc gatcgagacg tcgtgcgacg agacggccgc
     1741 cgcagtcgta gacgacggcg tgcacgcccg ctcgtcggtg gtgtcgagcc aagccgatct
     1801 gcacgcgcgt ttcgggggtg tggtgccgga gctggcgagc cgcgcgcatg tcgagttgat
     1861 cgacgacgtg atcgcggagg cgctcgtcga agccggttcg gatctcgggg agatcgacgc
     1921 ggttgcggca gtgcacggcc cgggtctcgc gggcgcgctg ctcgtgggcg tgagcgcggc
     1981 gaaagcgatc gcgctcgccg ccgaccgtcc gtacgtcggc gtgaaccacc acgaagcgca
     2041 catctacgcc gcgctgctcg aggacccgac gctgcaaccg ccgctcgtca cgttgatcgt
     2101 gtccggcgga cacaccttgc tcgtcgcgat gcgcgaccac ggcgactatg agatcgtggg
     2161 gcaaaccgtc gacgatgcgg cgggcgaagc gttcgacaag gtcgcgcgct tcctcgggct
     2221 cggatatccc ggcggtccca tcatcgaccg cctcgctgcg cagggcgatc cgaaggcggt
     2281 cgcgttcccg cgcccgatgc tcgacgacgg cgacgggttc tcgttctcgg ggttgaagac
     2341 cgcggtcgtc cagtggatgc gcaagcaccc cgacatggag atcgccgacg tcgccgcgtc
     2401 gttccaggcc gcggtcgtcg acgtgctggt gacgaagttg gtgcgggtcg cgacgcgcct
     2461 ggcggtgccg acggtcgtga tcggcggggg cgtcgcggcc aacacggcgc tgcgcacccg
     2521 gctcctcgac gcggccgaaa cggccgggct gcaagcggtg ctgccgagcc gcgacctgtg
     2581 caccgacaac gcggcgatgg tcggcgcggt cgcctaccgc cgcctgcagg ccgacgggcc
     2641 cacggccctg gacggcggcg tgtatcccaa tcttcggctg ggcttgcccc cggccggggc
     2701 ggcccggtag cgttagcact cgcacgggcc gagtgctaac gacggcacgc cggccggcac
     2761 gcttcgaaga ccctctcgag gaggcaatcc gcgatgaact tgcagccgtt ggaggaccgc
     2821 atcgtcgtcc gcccggccga ggccgaagag acgacgcaca gcggcctcgt cattcccgac
     2881 accgccaagg agaagcccca gcagggcgaa gtcctggcga tcggtccagg ccggcgcgcc
     2941 gagcagagcg gcgagctgat cccgctcgac atcgcggtag gcgacaccgt cgtctactcg
     3001 aagtacggcg gcaccgagat ctcccaagac ggcgaggacc tcttgatcct caacgcgcgc
     3061 gacgtgctcg ccaaggtcgc ggccggcaag aagaagaagt agttcccgcg acgacaagca
     3121 gtttgtgcag cgaggcggcc gcgaggccgc ctcgcttcgc gagcgcgcga gctacaccga
     3181 tcgcttcgcc gtctccttcg cccatttgta gtcggccttg ccgttgggcg cgcgctgcac
     3241 gacgggcacg aacacatagt gcttgggttg cttgaagcgt gagaggccgc ccagggtcga
     3301 gctgatctct tcgggtgtcg cggactggcc ggggtggaac gacacgaccg cggtgac
//
LOCUS       DASUGA010000613         2065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1206617, whole genome shotgun sequence.
ACCESSION   DASUGA010000613 DASUGA010000000
VERSION     DASUGA010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206617"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1625
                     /gene="murJ"
                     /locus_tag="VFX21_12140"
     CDS             <1..1625
                     /gene="murJ"
                     /locus_tag="VFX21_12140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX5096763.1"
                     /translation="VTEAAPAPHAGSRRLLRSSAVVGVGTALSRLTGFGRVAAIAYAL
                     GASTIAGVYSYANETPNIFYELLLGGILTATLLPLFVRNLHDGDDEATNAIVTVATVA
                     LLVISALGILLAPLIVRIYTLNVDGPNQDAQRQLATDWLRCFMPQIFFYGLTALATGM
                     LNARRRFAAAAFAPVLNNVVVIAMFLSLPRLVSGPITVDRVLHDPQLILYIGLGTTAG
                     IAAMALALWPALRHAGVRLRPVFAWRHTAVNTMIRLSGWTVGYVIVNQIALWVVLVLA
                     NGKSAGAFVYLSAYAFFQLPHGLFAVSITTAIQPDLASSYVANNWTGFRYRFARGLRL
                     IFTLMVPATALYLGLAQPVVAALLQRGAFNATATARVSDTLTAFAVGLPAFSVYLYSL
                     RAFYSMQDTRTPFLINGLENALNIVGALLLYPAMGIPGLALAFSLAYTIAAVVTLVVM
                     SRRLGSLRGRQIGSTFARVCVVGAAMFAVAWFVSHALGYGSPSAAILAALVGAVASVA
                     VGIIGLRVLRVRELTELRDAFRPATREVAVSE"
     gene            1663..>2065
                     /locus_tag="VFX21_12145"
     CDS             1663..>2065
                     /locus_tag="VFX21_12145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX5096764.1"
                     /translation="MGVRVVTDSSCDLSRNLIDAYGIEVVPLTIRFGDEELVDRDELD
                     PDEFWTRLKSSDALPETSAPSAGAFEARFRDLIQRGATGIISINLSSRLSATMQAAQV
                     AANVVRDDCPTVVIDSRTVSMGLGAHCLTAAR"
BASE COUNT          271 a          777 c          657 g          360 t
ORIGIN      
        1 tcgtgaccga agccgcgccg gcgccgcacg ccggctcgcg gcgcctgctg cgctcgagcg
       61 cagtggtcgg cgtcgggacc gcgctctcac ggctcaccgg cttcgggcgg gtcgcggcga
      121 tcgcctacgc gctcggtgcg tcgacgatcg ccggcgtcta ctcctacgcg aacgagacgc
      181 cgaacatctt ctacgagctg ctgctcggag gcatcctcac cgcgacgctg ctcccactct
      241 tcgtgcgcaa cctgcacgac ggcgacgacg aagccacgaa cgcgatcgtc accgtggcca
      301 cggtggcgct gctcgtcatc agcgcgctcg gcattctcct cgcaccgctc atcgtccgca
      361 tctacacgct caacgtcgac ggccccaacc aggacgcgca gcgccagctc gccaccgact
      421 ggctgcgctg cttcatgccg cagatcttct tctacgggct caccgcgctc gcaaccggca
      481 tgctcaacgc gcgccgccgg ttcgcggccg cggcgttcgc gccggtgctc aacaacgtgg
      541 tcgtgatcgc gatgttcctg tcactgcccc gactcgtgag cgggccgatc accgtcgatc
      601 gcgtgctgca cgatccgcag ctcatcctct acatcgggct cgggaccacg gccggcatcg
      661 ccgccatggc gctcgcgctg tggccagcac tccggcacgc cggcgtacgg ctgcggccgg
      721 tgttcgcgtg gcggcacacc gccgtcaaca cgatgatccg gctttccggg tggaccgtcg
      781 ggtacgtgat cgtgaaccag atcgcgttgt gggtcgtgct cgtgctcgcc aacgggaagt
      841 cggccggcgc gttcgtgtac ctcagcgcat acgccttctt ccagctcccc cacggcctgt
      901 tcgcggtctc gatcaccacc gcgatccagc ccgacctcgc gtcgtcgtac gtcgcgaaca
      961 actggaccgg gttccgctac cggttcgcgc gcgggctccg cctcatcttc acgttgatgg
     1021 tgcccgcgac cgcgctgtac ctcgggctcg cccagccggt cgtggccgcg ctcctgcagc
     1081 gtggtgcgtt caacgccacg gccacggcgc gcgtctccga cacgctgacc gcgttcgccg
     1141 tcggtctgcc ggcgttctcg gtgtacctct actcgctgcg cgccttctac tccatgcagg
     1201 acacgcgtac gccgtttctc atcaacggcc tcgagaacgc gctcaacatc gtcggcgcgc
     1261 tgctcctcta ccccgccatg ggcattcccg ggctcgcact cgcgttctcc ctcgcctaca
     1321 cgatcgccgc ggtcgtgacg ctcgtcgtca tgagccgccg tctcggcagc ctgcggggcc
     1381 ggcagatcgg atcgacgttc gcgcgcgtct gcgtcgtcgg ggcggccatg ttcgcggtcg
     1441 cgtggttcgt gtcccacgcc ctgggctacg gctcaccgtc ggcggcgatc ctcgccgcgc
     1501 tcgtcggcgc ggtcgcgagc gtcgcagtcg ggatcatcgg cctgcgcgtg ctgcgcgtgc
     1561 gtgagctcac cgagctgcgc gacgccttcc gtccggcgac gcgcgaggtc gccgtgtcag
     1621 agtaggcacg gtgcaccacc ggaacacgag gagagcgaat cgatgggcgt gagggtcgtg
     1681 accgacagtt cgtgcgatct ctcgcgcaac ctcatcgacg cctacggcat cgaggtcgtg
     1741 ccgctcacca tccgcttcgg cgacgaggag ctcgtcgacc gcgacgagct cgatcccgac
     1801 gagttctgga cgcgactgaa gagcagtgac gcgttgcccg agacgtcggc gccgtcggcc
     1861 ggtgcgttcg aagcccgctt tcgtgacctc atccagcgcg gcgccaccgg gatcatctcg
     1921 atcaacctgt cgtcgaggct gtcggccacg atgcaagccg cgcaggtggc ggccaacgtc
     1981 gtgcgcgacg actgcccgac cgtcgtcatc gactcccgca ccgtctcgat ggggctcggc
     2041 gcgcactgcc tcaccgccgc gcggc
//
LOCUS       DASUGA010000614         5393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1309130, whole genome shotgun sequence.
ACCESSION   DASUGA010000614 DASUGA010000000
VERSION     DASUGA010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309130"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VFX21_12150"
     CDS             complement(<1..520)
                     /locus_tag="VFX21_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096765.1"
                     /translation="MTITKRIRASTVNVALLAIGLLASFWLLYFEGEYHAELVLGIVA
                     VGALVALDQRDDPRFTIMTALMPFIVLLDIADVFYDPTRGIVVQGAEVGALVALLAVG
                     IALIYRANRVINFAQGDLGAAPAIFVLLLIAKDAPGGAPDWMTGLPYPVAFVLGLISA
                     VFLGFVVERLIIN"
     gene            complement(517..1116)
                     /locus_tag="VFX21_12155"
     CDS             complement(517..1116)
                     /locus_tag="VFX21_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5096766.1"
                     /translation="MTTAPVTDGRSARSQRTRGAVVDALLALLQGGNLRPTAREIAGR
                     AGVSLRSVYVHFDDLDDLFLTAAQRQMELVSGLLVEVPNTGRLRDRADTMMRVRGRIY
                     DQISAVRRAADVQRPFSPALAKYLDAVRDAARASLLHVFATELDRLDEPTRTARVAVL
                     EVLTAPEAWDHLRLVNEMDAPTVRTTIADHIVILLDGRA"
     gene            1178..3190
                     /locus_tag="VFX21_12160"
     CDS             1178..3190
                     /locus_tag="VFX21_12160"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5096767.1"
                     /translation="MAHPDVAAERARLRFAQECLDAMRERTAARIADEDVLAANKADA
                     DAVKWQLQRRLASLDDDASSLCFGRIDEESGDQWYIGRRHVEDGTGAPVVVDWRAEVA
                     TAFYRATLADPFGLHLRRRFIFNGRDLADVFDEDFSDPDSLAGTGGVPDPLLAELGRA
                     RTGQMRDIVATIQTEQDTIIRAPLERCLVVQGGPGTGKTAVGLHRAAFLLYEHRVLLA
                     REGVLVVGPNPVFLRYISQVLPSLGETSATQTTVDGLLSMRFRVVADDAYDVAAVKGD
                     ARMQTVIARAAEEHLRIPDDGIALAYRARTLRLSADELLDLAIAAQRENLAISIQRQR
                     FRDALIRAAYALYTRGELLGVDEKEFGTDLLANTDNRKQVDNCWKSVNPVGLVRSLLT
                     QKAALARAAAGVLTDEEQARLLRPRADAAAWTAHDLALLDEAETLVKAMPRRFGHVVV
                     DEAQDLSPMQLRMLARRARRHSMTVLGDLAQATGPASPGAWEETLVHLGRPANAQRAE
                     LTMGYRVPGAILELANRLLPHAAPDVAASRSVRESGDPPDFHEFGADELVPGVVDHAL
                     ALAKEFTTVAVIAEDSRIPELRDAIEQRGVVLGELGETSPEYPLVVVPASLAKGLEFD
                     AVLLVEPAEIMAREPHGVRLLFVALTRAVQHLGVAYVTPVPSVLRR"
     gene            3201..3872
                     /locus_tag="VFX21_12165"
     CDS             3201..3872
                     /locus_tag="VFX21_12165"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX5096768.1"
                     /translation="MTDDVMRVRIETRSGNVRLEAVEGAALEVHGGEVREERDGTTLV
                     KGGSRKLEVVVPSGADVTIGTHSGSVELHGWFGSARIATKSGSIEVDRAHAVDARTAS
                     GSVRVERCDGECRVVVVSGSVKIGRAGRASLACVSGSIEAGHVDDAEVKNVSGTTELG
                     ANATGRITVRSVSGKVKINVPSDRSPATSLKSVSGKIRCECDQGHDGEVDVKTVSGTI
                     QVACD"
     gene            3872..4372
                     /locus_tag="VFX21_12170"
     CDS             3872..4372
                     /locus_tag="VFX21_12170"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5096769.1"
                     /translation="MAEHGAIVFTDIVGFTELTDEHGDDVAIALLERQEVLVRAALPD
                     CSRVVKELGDGLLLWFDDARAALETCLELQHSFEHDDESVLPLWVRMGVHWGCPRPRG
                     DDIIGRDVNLASRIVDLAGPGEVLLSEAAADVIGDLKGVDFEALGPVFVRGFSDPVPL
                     VRAVPA"
     gene            complement(4381..>5393)
                     /locus_tag="VFX21_12175"
     CDS             complement(4381..>5393)
                     /locus_tag="VFX21_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096770.1"
                     /translation="HTSYFALFALRLAVTIALAVISYHLVEQPVRTGTRIRGTWPRVL
                     LPATGALLGVALLAVTASPPPPAFSLTPVAQHTPVTAPVTSATASTLAPAPTPTTAPA
                     GMHRALDTARPVRVMVVGDSVGITVGRGEELYANETGRVIVRNEARKWCSLGRYAPRI
                     MGYGESEQGDGCNDWATRWAGDIDEFDPDVVVVVYTVWEIAARKPVGAADWVKPGEPA
                     FHKWQLSEYQTAADVLSRRGATVVWTTVPCSNDDPSDYGSPVWWVNRDTIHALARSRR
                     DTVRLVDLEAELCPGHRPRAEYRGVDPARPDGRHFSDDGALALAQWMMPIVLGDAPSP
                     QP"
BASE COUNT          806 a         1828 c         1977 g          782 t
ORIGIN      
        1 ggttgatgat caaccgctcg acgacgaaac cgaggaagac cgccgagatc aacccgagta
       61 cgaaggcgac cggatagggg agtcccgtca tccagtcggg ggcgccgccc ggcgcgtcct
      121 tggcgatcaa gaggaggacg aagatcgccg gtgccgcgcc gagatcgccc tgggcgaagt
      181 tgatgacgcg gttggcccgg tagatcagcg cgatgccgac ggcgagcagc gcgacgaggg
      241 cgcccacttc cgcgccctgc acgacgatgc cgcgtgtggg gtcgtagaag acgtccgcga
      301 tgtcgagcag cacgatgaac ggcatgagtg ccgtcatgat cgtgaagcgc gggtcgtcgc
      361 gctggtcgag ggcgacgagc gcgccgaccg cgacgatgcc gaggaccagc tccgcgtggt
      421 actcgccttc gaagtagagc aaccagaacg acgccagcaa accgatggcg agcagcgcga
      481 cgttgacggt gctcgcgcgt atgcgtttcg tgatggtcac gcgcgcccgt ccagcaggat
      541 cacgatgtga tcggcgatgg tcgttcgtac ggtcggcgcg tccatctcgt tcacgagccg
      601 gaggtgatcc caggcctcgg gcgcggtcag cacctccagc accgcgacgc gcgcggtgcg
      661 tgtcggctcg tcgagccggt cgagctcggt cgcgaacacg tgcaggaggc tggcccgcgc
      721 cgcgtcccgc accgcgtcga ggtacttcgc gagcgccggc gagaacggtc gctgcacgtc
      781 ggcggcacgc cgcaccgcgc tgatctggtc gtagatccgg ccgcgcaccc gcatcatcgt
      841 gtcggcccgg tcccggaggc ggccggtgtt cggcacctcg acgaggagac ccgacaccag
      901 ttccatctgg cgttgtgcgg cggtgaggaa caggtcgtcg aggtcgtcga agtggacgta
      961 gaccgatcgc agggacaccc cggcgcggcc ggcgatctcg cgggcggtcg gccggaggtt
     1021 gccgccctgg agcaacgcga gcagggcgtc cacgacggcg ccgcgcgtgc gttgggagcg
     1081 ggcgctgcgg ccgtctgtga ccggtgcggt cgtcatcggg ccgtcaccat aaatatgcct
     1141 tcacaccctg tgcaagcacg gtagccttgc gccccctatg gcccacccgg acgtcgcggc
     1201 ggagcgcgcg cgtctgcggt tcgcacaaga gtgcctggac gccatgcgcg aacggacggc
     1261 ggctcggatt gcggacgagg acgtgctcgc ggcgaacaag gcggacgccg acgccgtgaa
     1321 gtggcagttg cagcggcgat tggcctccct cgacgacgac gccagctcgc tgtgcttcgg
     1381 ccgtatcgac gaagagtccg gcgaccagtg gtacatcggg cgccgccacg tcgaggacgg
     1441 caccggtgcg cccgtcgtcg tcgactggcg ggccgaggtg gccaccgcgt tctaccgggc
     1501 cacgctcgcc gaccccttcg gcctgcacct gcggcgccgg ttcatcttca acgggcgcga
     1561 cctcgcggac gtcttcgacg aggacttcag cgaccccgac tcgctggcgg gcacgggcgg
     1621 tgtgcccgac ccgctgctgg ccgaactggg acgtgcccgt accggccaga tgcgcgacat
     1681 cgtcgcgacg atccagaccg aacaggacac gatcatccgc gcgccgctcg agcgctgtct
     1741 cgtcgtccaa ggtggtccgg gcacgggcaa gacggccgtg gggctgcacc gcgccgcgtt
     1801 cttgctctac gagcaccgcg tgctgctggc ccgcgagggc gtgctcgtcg tcggcccgaa
     1861 cccggtgttc ctgcgctaca tcagccaggt gcttccgtca ctcggtgaga cgtcggcgac
     1921 ccagacaacg gtcgacggcc tgttgtcgat gcgcttccgg gtcgtggccg acgacgcgta
     1981 cgacgtcgcc gcggtaaagg gcgacgcgcg catgcagacg gtcatcgcgc gcgccgccga
     2041 agagcatctg cggatccccg acgacggtat cgcgcttgcc tatcgcgcgc gcacgctgcg
     2101 gctgagcgcg gacgagctgc tggatctcgc gatcgcggcg cagcgcgaga acctcgcgat
     2161 ctcgatccag cggcagcggt tccgggacgc gctgattcgg gccgcgtacg cgctctacac
     2221 gcgcggcgag ctgctcggcg tcgacgagaa ggagttcggt accgacctcc tcgcgaacac
     2281 cgacaaccgc aagcaggtcg acaactgctg gaagtcggtg aacccggtcg gtctcgtgcg
     2341 cagcctcctc acgcagaagg ccgcgctcgc acgcgcggcg gccggcgtgc tcaccgacga
     2401 ggagcaggcc cgactcttgc ggccgcgcgc cgacgcggcc gcgtggacgg cgcacgacct
     2461 cgcgttgctc gatgaggcgg agacgctcgt caaggcgatg ccgcggcgct tcggtcacgt
     2521 cgtcgtcgac gaagcgcaag atctctcgcc gatgcagctg cgcatgctgg cccgccgcgc
     2581 ccggcgccac tcgatgacgg tgctcggtga ccttgcccag gccaccggtc ctgcgtcgcc
     2641 cggtgcctgg gaggagacgc tggtgcacct gggccggcct gcgaacgcgc agcgcgcgga
     2701 gctcacgatg ggctatcgcg tccccggcgc gatcctcgag ctcgcgaacc ggctgctgcc
     2761 gcacgccgcg cctgatgtcg ccgcgtcccg atcggtgcgc gagtcgggag acccacccga
     2821 cttccacgag ttcggcgccg acgagctcgt gcccggcgtc gtcgaccacg ccctcgcgct
     2881 ggccaaggag ttcacgaccg tcgccgtgat cgccgaagac tcccgcatcc ccgaactgcg
     2941 cgacgcgatc gagcagcgcg gtgtggtgct cggtgagttg ggggagacct cacccgagta
     3001 ccccttggtc gtcgtgcccg cgtcgctggc caaaggtctc gagttcgacg cggtcctcct
     3061 cgtcgagccc gcggagatca tggcgcgcga gccgcacggc gtccgcctgc tcttcgtcgc
     3121 gctgactcgt gccgtgcagc acttgggtgt ggcatacgtc acacccgtgc cgtccgtact
     3181 gcggcgttag gcttgcgcgc gtgaccgacg acgtcatgcg cgtccgcatc gagacccgca
     3241 gcggcaacgt gcgcctcgaa gcggtcgagg gcgcggcact cgaagtgcac ggcggtgagg
     3301 tgcgcgagga gcgcgacggc acgacgctcg tgaagggcgg gtcgcgcaag ctcgaggttg
     3361 tcgttccatc cggcgcggac gtcacgatcg ggacgcacag cggctcggtc gagctccatg
     3421 gctggttcgg gtcggcgcgc atcgcgacga agagcggcag catcgaggtc gaccgtgccc
     3481 acgcggtcga tgcccgcacc gcgagcggca gcgtgcgggt cgagcggtgc gacggtgagt
     3541 gccgcgtcgt cgtcgtcagc ggcagcgtga agatcggccg cgcgggccgg gcctcgctcg
     3601 cctgcgtgtc gggctcgatc gaggccgggc acgtcgacga cgcggaagtg aagaacgtga
     3661 gcggcacgac ggagctcggc gcgaacgcga ccgggcggat cacggtgcgc agcgtgtcgg
     3721 gcaaggtgaa gatcaacgtg ccctccgatc ggtcaccggc gacgagcttg aaatcggtca
     3781 gcggcaagat tcggtgcgag tgcgaccagg gtcacgacgg cgaggtcgac gtcaagacgg
     3841 tcagcggcac catccaggtt gcctgcgact gatggcggag cacggcgcca tcgtcttcac
     3901 ggacatcgtc gggttcacgg agctgaccga cgagcacggc gacgacgtcg cgatcgcgct
     3961 gctggaacgg caagaagtgc tggtgcgcgc cgcgctgccg gattgcagcc gcgtcgtcaa
     4021 ggagctcggc gacggcctgt tgctgtggtt cgacgacgcc cgcgccgcgt tggagacgtg
     4081 cttggagctc caacacagct tcgagcacga cgacgagagc gtgctgccgt tgtgggtgcg
     4141 catgggcgtg cattggggtt gcccgcgccc gcgcggtgac gacatcatcg ggcgcgacgt
     4201 gaacctcgcg tcgcgcatcg tcgatctcgc gggcccgggc gaggtcctgc tctcggaagc
     4261 ggcggccgac gtgatcggcg acctcaaagg cgtcgacttc gaggcgctcg gaccggtgtt
     4321 cgtgcgcggc ttctccgatc cggtaccgct cgtgcgcgcc gtacctgcgt agccgcgcgt
     4381 ctacggctgc ggcgagggtg cgtcgccgag gacgatcggc atcatccact gcgcgagcgc
     4441 gagcgcgccg tcgtcggaga agtgccggcc gtcggggcgc gccggatcga cgccgcggta
     4501 ctccgcgcgc ggccggtggc ccgggcagag ctcggcctcg aggtcgacga gccgcaccgt
     4561 gtcgcgacgc gagcgcgcga gcgcgtgaat ggtgtcgcgg ttgacccacc agaccgggct
     4621 gccgtagtca ctcgggtcgt cgttcgagca cggcacggtc gtccacacga cggtcgcacc
     4681 gcgccgcgac aacacatcgg ccgcggtctg gtactcggag agctgccact tgtggaacgc
     4741 gggctcgccg ggcttcaccc agtcggcggc tcccaccggc ttgcgcgccg cgatctccca
     4801 caccgtgtac acgacgacga cgacgtcggg atcgaactcg tcgatgtcgc cggcccatcg
     4861 ggttgcccag tcgttgcagc cgtcgccttg ttcggactcg ccgtagccca tgatgcgcgg
     4921 cgcgtagcgc ccgaggctgc accacttgcg cgcttcgttg cgcacgatca cgcggccggt
     4981 ttcgttggcg tagagctcct cgccgcgccc gacggtgatg ccgaccgaat cgccgacgac
     5041 catgacgcgc accggacgcg cggtgtcgag cgcgcgatgc atcccggcgg gtgcggtcgt
     5101 cggggtcggc gcgggcgcaa gcgtcgacgc cgtggctgac gtgacgggcg cggtcacggg
     5161 cgtgtgctgt gcgaccggcg tgagcgagaa ggcgggtggc ggcggcgacg cggtgaccgc
     5221 gagcaacgcg acgccgagga gcgcgcccgt ggccggcaac agcacgcgcg gccaggtgcc
     5281 gcggatgcgc gtcccggtgc gcaccggctg ttcgacgagg tggtacgaga tcaccgcaag
     5341 cgcgatcgtc acggcgagcc ggagcgcgaa cagggcgaag tacgacgtgt gca
//
LOCUS       DASUGA010000615         2608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_337819, whole genome shotgun sequence.
ACCESSION   DASUGA010000615 DASUGA010000000
VERSION     DASUGA010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337819"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VFX21_12180"
     CDS             complement(<1..722)
                     /locus_tag="VFX21_12180"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5096771.1"
                     /translation="MSVAVPILCYHSVAAAAPSGFREWTIDPARFREHLEVIASRGAR
                     TPTVSEYAATLEADFPVANTVVLTFDDGFADFSTNVAPLLVEFDMRATLYVTTGFLGR
                     EPEWLPQEARAPMLSSRAISDLPDANVEIGAHAQCHPELDVIPRDAARAEIEGSKRAL
                     EDILGRPVASFAYPFGYHNRAVRQQVVDAGFTSACAVKQALSGPGDDIFALARVLIPG
                     DLTADDLARVLDAATVGRARAG"
     gene            complement(719..>2608)
                     /locus_tag="VFX21_12185"
     CDS             complement(719..>2608)
                     /locus_tag="VFX21_12185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5096772.1"
                     /translation="NRGIAVSTGDVVAFLDDDAVAADDWLARLLAPYADSNVVGAGGF
                     VEPRFDSDRPSWFPREFDWVVGCTYAGHRHSAGPVRNLIGATLSFRRETLVALGGFRD
                     GIGRVGTKPTGCEETELCIRARQGNAAAVIWYTPKARVSHRVPAGRTTWRYFRSRCVA
                     EGQSKARVAGVVGQDDALASERTYVTRTLPKASARDVRDRRWSRAGARVAGALLTASG
                     YAAERVRTREQAAFEPRLVADVDLGDTAAHLDDGERTTGRRYTRARVLVRNAGRPAGI
                     VDVELPEGGLHGAELRARLWPDASPVPEPPAPEVPSLSATVVIATRDRPAALRRCIES
                     LLAMNGPAFDIVVVDNAPATTETEELVGKLHATLGDCIRYVREDQPGLAAAHNRGLQE
                     VSGEIAAFTDDDVVVDADWLTMLLLAFDDPRVGCVTGLIAPLELETQVQLWIEDATGF
                     GKGLSRREFEPGEAPDDDPLFPFAAGRFGSGANMAFRTNALRAMGGFDPALGAGTKAR
                     GGDDLNAFARVVLHGHRLVYEPAAIVWHMHRRDIEGLRRQAFGYGAGLTAYLTSIVVE
                     KPARLPEIAKRAPGGLRYALDPSSPKNARIPSSQPRDLKYRELAGMALGPLLYTASRR
                     STRAR"
BASE COUNT          379 a          904 c          959 g          366 t
ORIGIN      
        1 tcaccggctc gcgcgcgacc gacggtcgcc gcgtcgagca cccgggcgag atcgtcggcg
       61 gtgaggtcac cgggaatcag cacgcgcgcg agcgcgaaga tgtcgtcgcc gggaccgctc
      121 aacgcctgct tgaccgcgca cgcgctggtg aagccggcgt cgacgacctg ttggcgtacg
      181 gcgcggttgt ggtagccgaa cggatacgcg aacgacgcca ccggtcgccc gaggatgtct
      241 tcgagcgcgc gcttgctgcc ctcgatctcg gcgcgcgcgg cgtcgcgcgg gatcacgtcg
      301 agctcgggat ggcactgtgc gtgtgcgccg atctcgacgt tggcgtcggg caggtcactg
      361 atcgcacgcg aagacagcat cggcgcgcgt gcctcttgcg gcagccactc gggctcgcgc
      421 ccgaggaagc ccgtcgtgac gtacagcgtt gcgcgcatgt cgaactcgac gagcagtggc
      481 gcgacgttgg tcgagaagtc ggcgaagccg tcgtcgaagg tcagcaccac ggtgttcgcg
      541 accgggaagt cggcttcgag cgtcgccgcg tactccgaga cggtcggcgt acgcgcgccg
      601 cgactcgcga tgacctccag atgctcgcgg aagcgcgcgg gatcgatggt ccactcgcga
      661 aagccggacg gcgccgcggc cgcgaccgag tggtaacaga gaatggggac cgcgacgctc
      721 atcgcgctct cgtcgaccgc cggctcgcgg tatagagaag tggtccgagc gccatgcccg
      781 caagctcccg gtacttgaga tcgcgcggct ggctcgacgg tatgcgggcg ttcttcggtg
      841 acgacgggtc gagcgcgtag cgcaagccgc ccggtgcacg cttggcgatc tcgggcagtc
      901 gtgcgggctt ttcgacgacg atgctcgtga ggtacgcagt cagtcccgcg ccgtagccga
      961 acgcttggcg ccgcagtcct tcgatgtcgc gtcggtgcat gtgccagacg atcgcggccg
     1021 gttcgtagac cagccggtga ccgtggagca cgacccgcgc gaacgcgttg aggtcgtcgc
     1081 cgccgcgcgc cttggtcccg gcgcccaacg cgggatcgaa gccgcccatg gcgcgcagcg
     1141 cgttcgtgcg gaacgccatg ttggcgcccg aaccgaagcg gccggccgcg aacgggaaca
     1201 gcgggtcgtc gtcgggcgcc tcgccgggct cgaactcgcg gcgcgacagc cccttgccga
     1261 aaccggtggc atcctcgatc cacaactgga cctgcgtttc gagctcgagc ggcgcgatca
     1321 ggccggtcac gcagccgacg cgcggatcat cgaacgcgag cagcagcatc gtcagccagt
     1381 ccgcgtccac gacgacgtcg tcgtcggtga aggcggcgat ctcgcccgag acctcttgca
     1441 gaccgcggtt gtgcgcggcc gcgagtccgg gctggtcctc gcgcacgtag cgaatgcagt
     1501 cgccgagggt tgcgtgcagc ttgccgacga gctcttcggt ctcggtcgtg gcgggcgcgt
     1561 tgtcgacgac gacgatgtcg aacgccggcc cattcatggc gagcagcgac tcgatgcagc
     1621 gccgcaacgc ggcgggacgg tcgcgggtcg cgatgacgac cgtcgcgctc agcgacggca
     1681 cctcgggcgc gggcggctcg ggaacgggcg acgcgtcggg ccacaggcga gcgcggagtt
     1741 ccgcgccgtg cagccctccc tccggcagct cgacgtcgac gatgcccgcg ggacgcccgg
     1801 cgttgcggac gaggacgcga gcgcgcgtgt agcgccgacc ggtcgtgcgc tcgccgtcgt
     1861 cgaggtgcgc ggccgtgtcc ccgaggtcga cgtcggcgac gagccggggc tcgaacgcgg
     1921 cctgctcgcg ggtccggacg cgttcggcgg cgtagccgct cgcggtcagc aatgcgccgg
     1981 cgacgcgcgc gccggcgcgt gaccagcggc ggtcccggac gtcgcgcgcg ctcgccttcg
     2041 gcagcgtgcg cgtcacgtac gtccgctcac tcgcgagggc gtcgtcctgg ccgacgacgc
     2101 ccgcgacgcg ggctttcgat tgaccttcgg cgacacagcg cgaacggaag tagcgccatg
     2161 tcgtacgtcc cgccgggacc cggtgcgaca cccgcgcctt cggcgtgtac cagatcacgg
     2221 cggcagcatt gccctgccgc gcgcggatgc acagctcggt ctcctcgcaa cccgtcggct
     2281 tcgtgccgac gcggccgatg ccgtcgcgaa acccgccgag cgccacgagc gtctcccgac
     2341 ggaacgacag ggtggcgccg atcaggttgc ggaccggtcc ggcggagtgg cggtgcccgg
     2401 cgtacgtgca cccgacgacc cagtcgaact cacgcgggaa ccacgacggc cgatccgagt
     2461 cgaagcgcgg ttcgacgaag ccgccggcac cgacgacatt cgagtccgcg tacggcgcga
     2521 gcaggcgtgc gagccagtcg tcggccgcga ccgcgtcgtc gtcgaggaag gcgacgacgt
     2581 cacccgtgct gaccgcgatg ccgcggtt
//
LOCUS       DASUGA010000616         2072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_94277, whole genome shotgun sequence.
ACCESSION   DASUGA010000616 DASUGA010000000
VERSION     DASUGA010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94277"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VFX21_12190"
     CDS             <1..546
                     /locus_tag="VFX21_12190"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5096773.1"
                     /translation="ELLARDLDGDLPFSVERRVLSNGSIYIGMAQLSDGQQALLAADD
                     APIDLAAFGVDPQQGFCRGVAATFSPSHPASEVEQEVQWLFASYADGHKLLPRTIRYI
                     EDRRAEEARFTGAIERHPSPLGVVWGRLDPIAVHAMTDRLLAARPDAILHTLDDVGHY
                     PMVEAPGPFADACLELIAKDV"
     gene            558..1379
                     /locus_tag="VFX21_12195"
     CDS             558..1379
                     /locus_tag="VFX21_12195"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEX5096774.1"
                     /translation="MDITLLGTGNPLPDPNRCGPSTLVRAGDAIFLFDAGRGVVQRMV
                     AAGVLPLQLTSVFLTHLHSDHITDLNDVVTTHWVMSFAPMSLPIIGPGRTQEVVDGML
                     AMLGPDIEYRLAHHADLTWRPQLPVTEVQSGVVYDEGGVRIVAAPTDHRPVEPTVGFR
                     LEHDGKVVVIAGDTVPCDGLDQLCANADAYVQTVLRADLIGASPSQRFRETVDYHSTV
                     EQAAQTAKRAGVKKLVLTHFVPGFPPGQGQEWADLAAAHYDGEIVLGEDLTTVTV"
     gene            1376..>2072
                     /locus_tag="VFX21_12200"
     CDS             1376..>2072
                     /locus_tag="VFX21_12200"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5096775.1"
                     /translation="MSTREADAAIERLARSLVPLPAQPAGTPWPTGAWPAGDPVPEIA
                     PLLDAMHADETRYGMTYATVVVVRGRLVHERYGGALPHFDRSATPVEPHTPLLSWSMA
                     KSVLHCAIGVLVGEGKLTLHAPAPVPAWANDERADITLEQLLCMRDGLAYNEDYVDAG
                     ESDVIEMLFGAGIHDVAGYAIARPLAHEPGTVFNYSSGTSNIVARILGDVVGSGDATR
                     AFLRRAVFDPIGMR"
BASE COUNT          313 a          765 c          682 g          312 t
ORIGIN      
        1 gagctgctgg cgcgcgatct cgacggcgac ttgccgttct cggtcgagcg ccgcgtgctg
       61 agcaacggca gcatctacat cgggatggcg cagctctccg acgggcagca ggcgctcctc
      121 gcggccgacg acgcaccgat cgacctcgcg gcgttcggtg tcgacccgca gcaagggttc
      181 tgccgcgggg tggcggcaac gttctcgccg tcgcatccgg cgtccgaggt cgaacaagag
      241 gtgcagtggt tgttcgcgtc gtacgccgac ggccacaagc tcctgccccg cacgatccgc
      301 tacatcgagg accggcgcgc cgaagaagcg cgcttcaccg gcgcgatcga acgccatccg
      361 tcaccgctcg gcgtcgtctg gggccggctc gatcccatcg cggtgcacgc gatgacggac
      421 cggctgctgg cggcgcgccc cgacgcgatc ctgcacacgc tcgacgacgt cggccactat
      481 ccaatggtcg aggcgcccgg tccgttcgcg gacgcgtgcc tcgagctcat cgccaaggac
      541 gtctgaagga cacgcgcatg gacatcacac tgctcggcac cggtaatcct ctgcccgacc
      601 cgaatcggtg cggcccgtcg acgctcgtgc gcgccggtga cgccatcttc ctgttcgacg
      661 cgggccgcgg cgtcgtgcag cgcatggtcg ccgcgggcgt cctgcccctc cagctgacat
      721 cggtcttcct cacgcacttg cacagcgacc acatcaccga cctcaacgac gtcgtcacga
      781 cgcactgggt catgtcgttc gcgccgatgt cgctgccgat catcggcccc ggtcgtacgc
      841 aggaagtggt cgacggcatg ctcgccatgc tcggccccga catcgagtac cggctcgcgc
      901 accacgccga tctgacatgg cggccgcaac tccccgtcac cgaagtgcag tcgggtgtcg
      961 tctacgacga gggcggggta cgcatcgttg ccgcgcccac cgaccaccgc ccggtcgagc
     1021 cgacggtcgg gttccggctc gagcacgacg gcaaggtcgt cgtcatcgcg ggcgacaccg
     1081 tgccgtgcga cgggctcgac cagctgtgcg cgaatgccga tgcgtacgtg cagaccgtgc
     1141 tgcgcgccga cctgatcggc gcgtcaccgt cccagcgctt ccgcgagacg gtcgactacc
     1201 actcgaccgt ggaacaggcc gcgcagacgg ccaaacgcgc gggcgtcaag aagctcgtgc
     1261 tcacccactt cgtcccgggc ttcccgcccg gtcaagggca ggagtgggcc gacctggcgg
     1321 ccgcgcacta cgacggcgag atcgtgctcg gagaagatct gaccacggtc acggtgtgag
     1381 cacgcgcgag gccgacgccg cgatcgagcg cctggcgcgg tcgctggtgc cgctgcccgc
     1441 gcaaccggcg ggtacgccgt ggccgaccgg cgcgtggccc gccggtgatc ccgttcccga
     1501 gatcgcgccg ctcctcgacg caatgcacgc cgacgaaacg cgctacggca tgacgtacgc
     1561 gaccgttgtg gtggtgcgcg gccgcctcgt acacgagcgg tacggcggcg cgctcccgca
     1621 tttcgaccgg tccgcgacac ccgtcgagcc ccacacgccg ttgctctcgt ggtcgatggc
     1681 gaagtcggtg ttgcactgcg cgatcggcgt gctcgtcggc gagggcaagc tcaccctcca
     1741 cgcaccggcg cccgttcccg cgtgggcgaa cgacgaacgc gccgacatca cgctcgagca
     1801 actgctgtgc atgcgcgacg gcctcgccta caacgaggac tacgtcgacg ccggcgagtc
     1861 cgacgtgatc gagatgctgt tcggcgccgg catccacgat gtcgcggggt acgcgatcgc
     1921 gcgcccactc gcacacgagc ccggcaccgt cttcaactac tcctcgggaa cgtccaacat
     1981 cgtcgcgcgc atcctcggcg acgtcgtcgg ctccggggac gcaacccgcg cgttcctccg
     2041 tcgcgccgtg ttcgacccga tcggcatgcg ta
//
LOCUS       DASUGA010000617         2714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_337959, whole genome shotgun sequence.
ACCESSION   DASUGA010000617 DASUGA010000000
VERSION     DASUGA010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337959"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="VFX21_12205"
     CDS             complement(<1..1452)
                     /locus_tag="VFX21_12205"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5096776.1"
                     /translation="MDTARRTLADVLRTHARSRPHALAFADGDVTLTWAQAATRVHRA
                     ANMLRAAGVQPGDRVLWLGQNSFRLEELLLACCELGAMFCPANWRQQPDELAFVIDDL
                     EPAVVVTQREEVGEAIAAARDLASSAHRARWIVHDDGEYDALVDAASQEDPVTDASDD
                     APLLLVYTAAFDGRPNAAMLPSRALIAQGLLMAPWTGVDATSVFMNSGPLFHIGTFMP
                     NLATFVSGGANVFVRRSDGDELCRAIDRYRCTDGFVLGPMVDAIVAANADRRYDLSSF
                     RGKRGNPQFDAWVSADPSPWGRRAGGYGQTETMGMATFNLLGVGAAGLHGRPSPLVDL
                     RVVDPDDNDVPVGETGEIAVRGLTVMCGYWNRPELNAVRTRNGWHHTNDLGRYEPDGS
                     FTFVGPKTRMLKSAAENIYPVEVENCIKTHPAVADCAVIGVPDEKWVQSVLAVVVTDG
                     TDVSAGEIVEHCRARMASYKKPRIVEFVDAIPRA"
     gene            complement(1462..2331)
                     /locus_tag="VFX21_12210"
     CDS             complement(1462..2331)
                     /locus_tag="VFX21_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096777.1"
                     /translation="MNLDSAWTHAPRLARARRRLVTLATIAVLAGCTLIALSTTQASA
                     NGEGITVDCEKVVAVKPMDKVRVRYLDNAEHTFDVASTLVFVFPTNNPHGRVKGVIVT
                     FDDGLEADRPVPACPATTTTTRPATTTTKPTTTTTRPATTTSTSASTSTTSSSTTTLP
                     TTTSTAATSTSTSTSTSTSTSTSTSTSTSTSTSSSTTSTTVGGNSSSLPPTSTTLAST
                     TTSTVGGLGSTVASLGSTVANTPTSVEPPSSSSTGLPVTGSGAGLFWLGVLSLGAGLA
                     TLVAARQLSRRTS"
     gene            complement(2439..>2714)
                     /locus_tag="VFX21_12215"
     CDS             complement(2439..>2714)
                     /locus_tag="VFX21_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096778.1"
                     /translation="STIGGLGSTSTTAQSGGGQATTSSSQVAGAEAGTSPTTVAGAKP
                     RGTEATGAAGGGSNLPFTGGNLTLLWAGLAALGAGGALYVNSRRRSV"
BASE COUNT          396 a          950 c          948 g          420 t
ORIGIN      
        1 ggcgcgcggg atcgcgtcga cgaactcgac gatgcgcggt ttcttgtagg acgccatgcg
       61 ggcccggcag tgctcgacga tctcgccggc gctgacgtca gtgccgtcgg tcacgacgac
      121 cgcgagcacc gactgcaccc acttctcgtc gggcacaccg atgaccgcgc agtcggcgac
      181 cgcgggatgg gtcttgatgc agttctcgac ttcgaccgga tagatgttct cggccgcgga
      241 cttcaacatg cgcgtcttcg gcccgacgaa cgtgaacgac ccgtcgggtt cgtagcgccc
      301 gaggtcgttc gtgtggtgcc agccgttgcg ggtccgcacc gcgttgagct ccggccggtt
      361 ccagtaacca cacatcacgg tgagaccgcg caccgcgatc tctccggtct cgccaaccgg
      421 aacgtcgttg tcgtcgggat cgacgacgcg caggtcgacg agcggcgacg gccggccgtg
      481 cagccccgcg gcgccgacgc cgagcaggtt gaacgtcgcc atgcccatgg tctccgtttg
      541 gccgtagccg cccgcccgcc ggccccacgg tgacgggtcg gcgctcaccc acgcgtcgaa
      601 ctgcgggttg ccgcgcttgc cgcggaacga cgacaggtcg tagcgacggt ccgcgttggc
      661 ggcgacgatc gcgtcgacca tcggcccgag tacgaacccg tcggtacagc ggtaacggtc
      721 gatcgcccgg cacagctcgt cgccgtcgga tcggcgcacg aacacgttgg caccgccact
      781 cacgaacgtc gcgaggttcg gcatgaacgt gccgatgtgg aacagcggac cggagttcat
      841 gaacaccgac gtcgcgtcga caccggtcca cggcgccatc aacagaccct gggcgatcag
      901 cgcgcgcgac ggcagcatcg ccgcgttggg ccggccgtcg aacgcagccg tgtagacgag
      961 caggagcggc gcgtcgtcgg acgcgtcggt caccggatcc tcttgcgacg cggcgtcgac
     1021 gagcgcgtcg tattcgccgt cgtcgtggac gatccagcgc gcccggtgtg cactcgacgc
     1081 gagatcacgg gcggccgcga tggcctcacc gacctcttcg cgctgggtga cgacgaccgc
     1141 aggctcgagg tcgtcgatca cgaacgcgag ctcgtcgggc tgttgacgcc agttcgcggg
     1201 acagaacatc gcgcccagct cgcagcacgc gagcagcagc tcctcgagcc ggaacgagtt
     1261 ctggccgagc cagagcacgc gatcgccggg ttgcacgccg gccgcgcgca gcatgttcgc
     1321 cgcccggtgt acgcgcgtcg cggcctgcgc ccacgtcagc gtcacgtcac cgtcggcgaa
     1381 ggcgagcgcg tggggacgcg agcgcgcgtg cgtccggagg acgtcggcca gcgttcggcg
     1441 agcagtgtcg atgcgcggaa gctagctggt gcgccgcgac agctgccgcg ccgccaccaa
     1501 cgtcgccagg ccggcaccga gcgacagcac gccgagccag aacagcccgg cgccactgcc
     1561 cgtcaccggc agaccggtcg acgacgacga cggcggctcc accgacgtcg gcgtgttggc
     1621 gacggtcgag ccgagcgatg cgacggtcga gccgaggccg ccgaccgtcg acgtggtcgt
     1681 cgacgcgagc gtcgtgctcg tcggcggcag cgacgacgag ttgccgccga ctgtcgtgga
     1741 ggtcgtgctg ctcgacgtgg aggtggacgt gctggtcgac gtgctcgtgc tcgtcgacgt
     1801 gctggtcgac gtgctcgtgc tggtgctcgt ggctgcggtc gacgtcgtcg tcggcagcgt
     1861 cgtcgtcgag ctcgacgtcg tcgacgtgct cgcgctcgtc gacgtggtcg ttgccggcct
     1921 cgtcgttgtc gtcgtcggct tggtggtggt cgtcgccgga cgcgtcgtcg tcgttgttgc
     1981 cgggcacgcg ggcaccggcc ggtccgcctc gaggccgtca tcgaacgtga cgatcacacc
     2041 tttgacccgg ccgtgcggat tgttcgtcgg gaacacgaac acgagcgtcg aagcgacgtc
     2101 gaaggtgtgt tccgcgttgt cgaggtaccg cacgcgcacc ttgtccatgg gcttcaccgc
     2161 gacgaccttc tcgcagtcga ccgtgatgcc ttcaccgttc gcgctcgcct gcgtcgtcga
     2221 gagcgcgatc agcgtgcatc ccgcgagcac tgcgatcgtc gccagcgtga ccagccggcg
     2281 gcgggcacgc gccagccgcg gggcgtgcgt ccatgccgag tcgagattca tacgtggggt
     2341 cccttcgcgc ctacaacacg gcaccccacg agccagcttg caaccctaga tgggagatcg
     2401 gcagaactgg tggattctgg caggagttcg gacggaagtc agacgctccg tcgccggctg
     2461 ttgacgtaca gcgcgccgcc cgcgcccagc gccgccagac cggcccagag cagcgtcagg
     2521 ttgccaccgg tgaagggcag gttcgacccg ccgccggccg caccggtcgc ctccgtcccc
     2581 cgaggcttgg ccccggcgac ggtcgtcggg ctcgtgcctg cctctgcgcc cgcgacctgc
     2641 gacgacgacg tcgtcgcctg accaccaccg ctctgcgcgg tcgtcgatgt cgaacccaga
     2701 ccgccgatcg tcga
//
LOCUS       DASUGA010000618         3265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_477459, whole genome shotgun sequence.
ACCESSION   DASUGA010000618 DASUGA010000000
VERSION     DASUGA010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477459"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VFX21_12220"
     CDS             complement(<1..597)
                     /locus_tag="VFX21_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEX5096779.1"
                     /translation="MGFFERNRQSLLEKGYSPDRLPPGQYFTERYPVLHVGDVPDIAP
                     HDWSVRVFGLVNNARTFTWDDLHAMDIVTITTDIHCVTKWSKFDTVWEGVRVREVLDA
                     VGVDARATHVLQHAAYGYTTNTPLAEVLADEALLAWKFGGEPLEPVHGGPVRMVIPRL
                     YFWKSAKWLEGLELLDHDEPGFWEKNGYHNEGDPWREQR"
     gene            complement(608..1069)
                     /locus_tag="VFX21_12225"
     CDS             complement(608..1069)
                     /locus_tag="VFX21_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX5096780.1"
                     /translation="MRITAKADYAVRAAVELAAAEASGRPVKGEALAKAQGIPQNFLE
                     NILTELRRAGIIRTRRGADGGYQLARPASEITVADVLRAVEGPLAAVQGVRPDQLAYG
                     GAAETLPEVWVALRASLRDVLEHVTVADIAHGKLPAVVKDRTRVKDAWRAH"
     gene            1306..2214
                     /locus_tag="VFX21_12230"
     CDS             1306..2214
                     /locus_tag="VFX21_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5096781.1"
                     /translation="MTLKLGYFPNVTHAPALIAKDKGYFEDKLGSNVKIDYKTFNAGS
                     EAVTAILAGALDASFVGPNPAINAFQKTKGEVRIVSGIATGGAFLVVKPGINSAADLK
                     GKKVATPQAGNTQDVALRTWLNDNGVSVTKDSGEVTITPQENSVTLTAFQQGQIDGAW
                     VPEPWATRLVQEGGGKILVDEASLWPDGKYTTTVLLVTKKFLDANPTVVENLIAAANE
                     GIKLVSSDPAAATASVSKGIGDVTGKPLKEELITSSFKSVKFGLDPVISSLETSAKNA
                     EKLGFLESSDIAGIYDSTQVDKVAKT"
     gene            2211..3002
                     /locus_tag="VFX21_12235"
     CDS             2211..3002
                     /locus_tag="VFX21_12235"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006545462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5096782.1"
                     /translation="MTALSHVEQQSPLGTPAISIAGVSTVFGHGNASVLALDHVSLDI
                     ARGEFVCLLGASGCGKSTLLNLVADLDRPSTGAVDAHGARVGLMFQEAALFPWLTVRG
                     NVELALKLAGKGRDERRARADELLAMVHLSEFAKRQPHELSGGMRQRVALARALAQDV
                     DVLLMDEPFGALDAMTRDRLHEELESIWQRTGTTVMFVTHNVREAVRLGDRVVLLASR
                     PGRVAEIFDVEIPRPRRIDSTDVATLAATITDRLREEASGGNRND"
     gene            2983..>3265
                     /locus_tag="VFX21_12240"
     CDS             2983..>3265
                     /locus_tag="VFX21_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006545461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5096783.1"
                     /translation="MATATTDIDELEVLFNARPSRPALYRRAWAAVWPKLVATAIFIG
                     AWQALVWTGWKPEYILPSPFTVFDAFFHNFGDLAASAAVTLKRGLVGYAI"
BASE COUNT          561 a         1109 c         1046 g          549 t
ORIGIN      
        1 ccgctgctcc cgccagggat caccctcgtt gtggtagccg tttttctccc aaaacccggg
       61 ctcgtcgtga tcgagcagct ccaagccttc gagccacttc gccgacttcc agaaatacag
      121 gcgcgggatc accatccgaa ccggcccgcc gtggaccggc tcgagcggct ccccgccgaa
      181 cttccacgcg agcagcgcct cgtcggcgag cacctccgcg agcggcgtgt tcgtcgtgta
      241 gccgtaggcc gcgtgttgga gcacgtgcgt ggcacgcgca tcgacgccaa cagcgtccag
      301 cacctcgcgc acgcgcacgc cctcccaaac cgtgtcgaac ttcgaccact tggtcacgca
      361 gtggatgtcg gtcgtgatcg tcacgatgtc catcgcgtgc aggtcgtccc acgtgaacgt
      421 gcgcgcgttg ttcacgaggc cgaacacccg cacgctccaa tcgtggggcg cgatgtcggg
      481 cacgtcaccg acgtgcagca ccggatatcg ctcggtgaag tactggccgg gcggcaggcg
      541 gtccgggctg tagccctttt cgaggaggga ttggcggttg cgttcgaaga atcccacggt
      601 gactccccta gtgcgcgcgc caggcgtctt tcacgcgcgt gcggtccttt accacggcgg
      661 gcagtttgcc gtgcgcgatg tcggcaaccg tcacgtgctc gaggacatcg cgcaggctcg
      721 cgcgcaaggc cacccacact tcgggcagcg tctcggccgc gccgccgtac gccagctgat
      781 cggggcggac gccctgcacc gcggccagcg gaccctcgac cgcacggagc acgtcggcca
      841 ccgtgatctc actcgccggg cgcgccagct gatacccacc gtcggctccc cggcgcgtgc
      901 ggatgatgcc ggcccggcgc agctcggtga ggatgttctc gagaaagttc tgcggaattc
      961 cctgcgcctt cgcgagcgcc tcacccttca ccggtcggcc gctcgcttcc gcagccgcca
     1021 gttcaaccgc ggcccggacc gcgtagtcgg ccttggccgt gatgcgcatg ccccgagtct
     1081 cgcaaacctc ctccggagtt gacggcaccg tgccgatact ctactttgtc gactggttta
     1141 gtcgacattt cgagttcgga ggttctcgtg ctgcaggttc gatcccggcg acgtctgctc
     1201 gccccgctca ccctcgccct ggctgcggtg gtgatcgcgg cgtgtggcag tagctcgaag
     1261 gatgcgtcgt cggggagttc cggaggcggc gacaccgaga agtcggtgac gctcaagctc
     1321 gggtacttcc ccaacgtcac gcacgcgcct gcactcatcg ccaaggacaa gggctacttc
     1381 gaagacaagc tcggctccaa cgtcaagatc gactacaaga cgttcaacgc gggctccgaa
     1441 gccgtgacgg cgatcctcgc cggcgcgctc gacgcgtcgt tcgtcggacc gaaccccgcg
     1501 atcaacgcgt tccagaagac caagggcgag gtgcgcatcg tctccggcat cgcgaccggt
     1561 ggcgcgttct tggtcgtgaa gcccgggatc aactcggccg ccgacctgaa gggcaagaag
     1621 gtagcaacgc cgcaggccgg taacacgcag gacgtcgcgc tgcgcacgtg gctcaacgac
     1681 aacggcgtgt cggtcaccaa ggacagcggt gaagtcacga tcacaccgca ggaaaactcc
     1741 gtgacgctca ccgcgttcca gcagggtcag atcgacgggg cgtgggtgcc ggaaccgtgg
     1801 gcgacccggc tcgtgcaaga aggcggcggc aagatcctcg tcgacgaggc gtcgctgtgg
     1861 cccgacggga agtacacgac cacggtcttg ctcgtcacca agaagttcct cgacgcgaac
     1921 ccgacggtcg tcgagaactt gatcgcggcg gcaaacgaag gcatcaagct cgtgagctcg
     1981 gatccggcgg ccgcaactgc atcggtcagc aagggcatcg gcgacgtcac cggcaagccg
     2041 ttgaaggaag aactgatcac gtcgtcgttc aagagcgtca agtttgggct cgacccggtc
     2101 atttcgtcgc tcgaaacctc ggccaagaac gcggagaagc tcggcttcct cgaatcgagc
     2161 gacatcgccg gaatctacga ctcgacgcaa gtggacaagg tggcaaagac atgacggcgt
     2221 tatcgcacgt cgaacaacag tcccccctcg gaacgccggc gatttccatc gccggcgttt
     2281 cgacggtttt cggacacggc aacgcgagcg tgctcgcact cgatcacgtc agcctcgaca
     2341 tcgcacgagg cgagttcgtc tgcctgctcg gcgcctcggg ttgcggcaag tcgacgctgc
     2401 tgaacctcgt ggctgatctc gaccggccga gcacgggcgc ggtcgacgcg cacggcgcgc
     2461 gggtcgggct gatgttccag gaagcagcgt tgtttccgtg gctgacggtc cgtggcaacg
     2521 tcgagctggc gctcaagctc gcgggcaagg gacgcgacga gcgacgcgcc cgggccgacg
     2581 aactgctggc gatggtgcac ctcagcgaat tcgccaagcg tcagccccac gaactctccg
     2641 gcggcatgcg ccagcgcgtc gcactcgctc gcgcattagc gcaagacgtc gacgttttgt
     2701 tgatggacga gccgttcggt gcgctcgacg ccatgacgcg tgaccggctg cacgaagagc
     2761 tcgagtccat ctggcagcgc accggcacca cggtgatgtt cgtcacgcac aacgtgcgcg
     2821 aggccgttcg cctcggcgat cgcgtggtgc tgctcgcgag ccggccggga cgggtcgccg
     2881 agatctttga cgtcgagatt ccacgccccc ggcgcatcga ctcgactgac gtcgcgacgc
     2941 tggccgcaac catcactgat cgtttgcgag aggaggcctc cggtggcaac cgcaacgact
     3001 gacatcgacg aactcgaagt cctgttcaac gcacggccgt cacgtccggc gctctaccgt
     3061 cgcgcgtggg ccgcggtgtg gccgaagctc gtcgccaccg cgatcttcat cggcgcatgg
     3121 caggcactcg tctggaccgg ctggaagccg gagtacatcc ttccgtcgcc cttcaccgtc
     3181 ttcgacgcgt tctttcacaa cttcggcgac ttggccgcat cggccgcggt cacgctgaag
     3241 cgcggactcg ttggttacgc catcg
//
LOCUS       DASUGA010000619         2070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_668087, whole genome shotgun sequence.
ACCESSION   DASUGA010000619 DASUGA010000000
VERSION     DASUGA010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668087"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VFX21_12245"
     CDS             <1..311
                     /locus_tag="VFX21_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010273729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096784.1"
                     /translation="ASQAIGYKEILDHLEGETPLDDAFDLVVRRTRRFARRQRVWFAR
                     DPRITWLDAGAAKTDVVAETVLATWSAPIPSGREADRRPEPQAPIREGAGGKRVASSP
                     "
     gene            316..1179
                     /gene="dapF"
                     /locus_tag="VFX21_12250"
     CDS             316..1179
                     /gene="dapF"
                     /locus_tag="VFX21_12250"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX5096785.1"
                     /translation="MTTLHLAKLHATGNDFLVALALEAGDPRLDNVTVAALCDRRRGI
                     GADGLITITAGRDGADCTMTLVNADGGVAEMSGNGIRCLAWVAARAGLATADELVVDT
                     AAGRRVVALERSGGTVIAADVDMGPPIFEPSRSPIDVDSPFGLDALVDGVAFRGDAVG
                     MGNPHWVLFVDDPATVPVAQLGPAFEHDRRFPRRTNVEFVKVEDRGRLTMRVWERGVG
                     ETLSCGTGACASAVVARRRDLVGEVVIVDVRGGSLTVNVGDTIRLGGPVVHIFDVELS
                     LEHLQAALDRS"
     gene            1176..>2070
                     /gene="hflX"
                     /locus_tag="VFX21_12255"
     CDS             1176..>2070
                     /gene="hflX"
                     /locus_tag="VFX21_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014694097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX5096786.1"
                     /translation="MTSSSRGHQARQRRGLAATEVDLERPRQRALLVGTGFDARSVEE
                     AEQSLEELALLTETAGHDPVELALQRRATPHAATYIGKGKAEELRALTEALDLDVVIF
                     DDELTPAQQRNLEKIFKVDVVDRAALILDIFAQHATSQEGAVQVELAQLRYRLPRLRG
                     RGVEMSQQGFGINARGPGETQLEVDRRRLLRRIQKLEKDLKQLAATRDTQRKARRRGG
                     LPRVALIGYTNAGKSTLLNRLTHAGVLVENQLFSTLDPTTRRLHLPGGETVLLSDTVG
                     FVRRLPHQLVEAFCSTLEEVAD"
BASE COUNT          309 a          742 c          721 g          298 t
ORIGIN      
        1 cggcgagcca ggcgatcggt tacaaggaga tcctcgacca cctggagggc gagaccccgc
       61 tcgacgacgc gttcgacctc gtcgtgcggc gcacccggcg cttcgcccgc cgccagcggg
      121 tttggttcgc acgcgacccg cgcatcacgt ggctcgacgc cggtgccgcg aaaaccgacg
      181 tcgtggccga gaccgttttg gcaacctggt cggcacccat tccgtccggg cgggaagcgg
      241 accggaggcc cgagccgcag gccccgatcc gagagggagc gggtgggaaa cgagtggcat
      301 catccccgtg acgacatgac gacgctgcac ctcgccaaac tccacgcgac cggcaacgac
      361 ttcctcgtcg cgctcgcgct cgaagccggc gacccccgcc tcgacaacgt cacggttgcc
      421 gcgctgtgcg accgccggcg tggcatcggt gccgacgggc tgatcacgat caccgccggc
      481 cgcgacggcg cggactgcac gatgacactg gtcaatgccg acggtggtgt cgctgagatg
      541 agcggcaacg gcatccgctg cctcgcctgg gtcgcggcgc gcgccgggct ggccaccgcc
      601 gacgagctgg tggtcgacac cgcggccggg cggcgcgtcg tcgcgttgga acgttcgggc
      661 ggcaccgtca tcgcggccga cgtcgacatg ggcccgccga tcttcgagcc gtcgcgcagt
      721 cccatcgacg tggactcacc cttcgggctc gacgcgctcg tcgacggcgt cgcgttccgc
      781 ggcgacgcgg tcggcatggg caacccgcac tgggtgctgt tcgtcgacga ccccgcgacc
      841 gtccccgtcg cacagctcgg ccccgcgttc gaacacgatc gccgcttccc gcgccgcacc
      901 aacgtcgagt tcgtgaaggt cgaagaccgg ggccgcctca ccatgcgcgt ttgggaacgc
      961 ggcgtcggcg agacgctctc gtgcgggacg ggcgcgtgcg cgagcgcggt cgtggcccgg
     1021 cgccgtgacc tcgtcgggga ggtcgtcatc gtcgacgtgc ggggcggttc gttgaccgtc
     1081 aacgtcggtg acaccatccg cctcggcgga ccggtcgtgc acatcttcga cgtcgagctc
     1141 tccctcgaac atctgcaggc cgcgttggat cgctcgtgac gtcgtcctcg cgcggccatc
     1201 aggcgcggca gcgccgaggg ctcgcggcga ccgaggtcga cctcgagcgc cctcgccagc
     1261 gcgcgctctt ggtcggtacc gggttcgacg cgcggtcggt cgaggaagcg gagcagtcgc
     1321 tcgaggaact ggccctcctc accgagactg ccggccacga cccggtggag ctcgcgttgc
     1381 agcggcgcgc gacaccacac gcggccacgt acatcggcaa gggcaaggcc gaagagttgc
     1441 gcgcgctcac cgaagcgctc gacctcgacg tcgtgatctt cgacgacgag ctcacgcccg
     1501 cgcagcagcg caacctcgag aagatcttca aggtcgacgt cgtcgaccgc gccgcgctga
     1561 tcctcgacat cttcgcccag cacgcgacga gccaggaggg cgcagtccag gtcgagctcg
     1621 cgcagttgcg gtaccggttg ccgcgcctgc gcggtcgcgg tgtggagatg agccagcagg
     1681 gcttcgggat caacgcccgt ggcccgggtg agacgcagct cgaggtcgac cgccggcgct
     1741 tgctgcgccg catccagaag ctcgaaaagg acctgaagca actcgcggcg acccgcgaca
     1801 cgcagcgcaa ggcccgtcgc cgcggtgggc tgccgcgagt cgcgctgatc ggctacacca
     1861 acgcgggcaa gtcgaccttg ctgaaccggc tcacccacgc gggcgtgctc gtcgagaacc
     1921 agttattctc gacgctcgat ccgacgacgc gccggctgca tctgcccggc ggcgagacgg
     1981 tgctgttgtc cgacacagtc ggtttcgtgc gccggttgcc gcaccagctc gtcgaggcgt
     2041 tctgctccac gctcgaggag gtggcggacg
//
LOCUS       DASUGA010000620         3232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1589143, whole genome shotgun sequence.
ACCESSION   DASUGA010000620 DASUGA010000000
VERSION     DASUGA010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589143"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VFX21_12260"
     CDS             <1..744
                     /locus_tag="VFX21_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096787.1"
                     /translation="EHGAPYFSSFLLADPRTAWIVETSGRTWAARQIPAGGAISNRIS
                     LTTDWDRASADVAPGANFDAWRRPMTPTNIADHRLAATTATVARGTAVDARALARTMR
                     DHGEAPAVPAEPGSDGRGVTVCLHVRGLQATTASMITELRDDGAPARVWLALGSPCVS
                     VYVPAFLPAVPVELAAEAQWFRFAQLRRRVEGWADALAAVQEVLAPVEAALWDEADEL
                     ARSGDVSAMARFAKRAYEPVDAALQRLGV"
     gene            755..2794
                     /locus_tag="VFX21_12265"
     CDS             755..2794
                     /locus_tag="VFX21_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX5096788.1"
                     /translation="MPNRLAQETSPYLRQHQDNPVDWYPWGDEAFAVARERGVPIFLS
                     VGYSSCHWCHVMAHESFEDDTTAQLMNERFVNVKVDREERPDVDAIYMQAVQAMTGRG
                     GWPMSVWLTHDGRPFYGGTYYPDQDRHGMPSFTRVCTALSAAWRDKPEEIAEQAEQLT
                     NAIDADVLRNASGGALSTELLRAAYQGVASQFEPRYGGFGHAPKFPQAMTVDFVCRAS
                     LRNRSPETLQILTTTLDAMAAGGMYDQVGGGFARYSVDDFWLVPHFEKMLYDNALLTR
                     AYLHGFQRTGNERYRRIVTETIEYVLRDLRDPSGGFYAAEDADSEGVEGKFYVWSLEE
                     VREVAGDDADEAIRYYGVTVGGNFVDPHTGFSGNVLHVVARTEEPNDAVRRAREKLFA
                     RRTTRVRPGLDDKVLLAWNALFLDALTEAATAFERDDWMDAARTNARFLVRELRRADG
                     RWLRSWRAPYLAFAEDYAALLEALCTLAELDDLGWLEQAREVADELVRLFADAEGDGF
                     FTTGHDAETLIIRQKDVFDDATPSANAMAANGLLRLSALTGDNAYDTRARAWLARVGP
                     TMASHPTAFAHSLQALERAVTPPVEVVVIGDPTNDDARALWREVTARLRPNTVVVRGA
                     APDPAPTTPLLEARTLVGGAPTAYVCEGYACRQPVTDVQELRGQLDAVLGQQAAT"
     gene            complement(2776..>3232)
                     /locus_tag="VFX21_12270"
     CDS             complement(2776..>3232)
                     /locus_tag="VFX21_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096789.1"
                     /translation="GVARKGKNAGAARTVLTGELSRRAAISFPAEDVLVATRLAQPSA
                     YTLLQTYANVCPRPGYTPNGEDRAWGRRLAKRFCLDAGYDERTFKTAIKGRKPPCVPI
                     LDASTIKATGGAKIVSLVGDVDAARGQAMIAFGWAVAEDLAAGNYVAAC"
BASE COUNT          493 a         1157 c         1125 g          457 t
ORIGIN      
        1 gagcacggcg cgccgtactt ctcgtcgttc ctgctcgccg acccgcgcac ggcctggatc
       61 gtggagacga gcgggcggac gtgggcggcg cggcagatcc ccgccggcgg cgcgatctcc
      121 aaccgcatct cactcacgac cgactgggac cgagcgtccg ccgacgtcgc gccgggcgcg
      181 aacttcgacg cgtggcggcg cccgatgacg ccgaccaaca tcgccgacca tcgcctggcg
      241 gcgacgacgg cgaccgtggc ccgcggcacg gctgtcgacg cccgtgcact cgcgaggacg
      301 atgcgcgacc acggcgaggc gcccgcggtg ccggcggagc ccgggagtga cggacgcggc
      361 gtcaccgtgt gcttgcacgt gcgcggcctg caggcgacga cggcgtcgat gatcaccgag
      421 ctgcgagacg acggcgcgcc ggcacgcgtg tggctcgcgc tggggagccc atgcgtgagc
      481 gtctacgttc ccgcgttcct gcccgccgtg ccggtcgagc tcgccgccga agcccagtgg
      541 ttccgcttcg cgcaactgcg ccgccgggtc gaaggttggg ccgacgcgct ggccgccgtg
      601 caggaagtcc tcgcaccggt cgaggcggca ttgtgggacg aggccgacga actcgcccgg
      661 tccggcgatg tctccgcaat ggcgcgcttt gcgaaacgcg catacgaacc ggtcgacgcc
      721 gctctgcaac gcctcggcgt ctagcgtgcg cgcggtgccg aaccgcctcg cgcaggaaac
      781 gagcccgtac ctccgccagc accaggacaa cccggtcgac tggtacccgt ggggtgacga
      841 ggcgttcgcc gtcgcgcgcg agcggggcgt gccgatcttc ttgtcggtcg ggtattcgtc
      901 gtgtcactgg tgccacgtga tggcgcacga gtcgttcgag gacgacacca ccgcgcaact
      961 catgaacgag cggttcgtca acgtcaaggt cgaccgtgaa gaacggcccg acgtcgacgc
     1021 gatctacatg caagcggtgc aggcgatgac cggccgcggc ggctggccga tgagcgtgtg
     1081 gttgacccac gacggccgcc cgttctacgg cggcacctac taccccgacc aagacaggca
     1141 cggcatgccg tcgttcacgc gtgtgtgcac ggcgctctcc gctgcctggc gcgacaagcc
     1201 cgaggagatc gcggaacagg ccgaacagct cacgaacgcg atcgacgcgg acgtgctgcg
     1261 caacgcgtcg ggtggcgcgc tgtcgacgga gctgcttcgc gccgcgtacc agggtgtcgc
     1321 gagccagttc gagccgcgtt acggcggctt cgggcacgcg ccgaagttcc cacaagcgat
     1381 gaccgtcgac ttcgtgtgcc gcgcatcgct tcgcaaccgg tcgcccgaga cgttgcagat
     1441 cctcacgacg acactcgacg caatggccgc gggcggcatg tacgaccagg tcggtggcgg
     1501 cttcgcccgc tactcggtcg acgacttctg gctcgtcccg cacttcgaga agatgctcta
     1561 cgacaacgcg ctgctgaccc gcgcgtacct gcacggcttc cagcgcaccg gcaacgagcg
     1621 ttaccgccgc atcgtcaccg agacgatcga gtacgtgctc cgcgacctgc gggatccgag
     1681 cggcgggttc tacgcggccg aagatgccga ctccgagggt gtcgagggca agttctacgt
     1741 ctggagcctc gaagaagttc gcgaagtcgc cggcgacgac gccgacgagg cgatccgtta
     1801 ctacggcgtc accgtgggcg ggaacttcgt cgacccgcat acggggttca gcggcaacgt
     1861 gctccacgtg gtcgcccgca ccgaggaacc gaacgacgcg gtgcggcgcg cccgcgagaa
     1921 gctcttcgcc cggcgtacga cacgggtgcg ccccggcctc gacgacaagg tgctcctcgc
     1981 gtggaacgcg ctgttcctcg acgcgctcac cgaggccgcg accgcgttcg aacgcgacga
     2041 ctggatggac gcggcgcgga cgaacgcgcg cttcctcgtg cgcgagctgc ggcgcgccga
     2101 cggccgctgg ctacgctcgt ggcgggcgcc ctacctcgcg ttcgccgagg actatgcggc
     2161 actgctcgaa gcgctctgca cgctggccga gctcgacgat ctcggctggc tcgagcaggc
     2221 ccgcgaggtc gccgacgaac tggtgcgcct cttcgccgat gccgagggcg acggtttctt
     2281 cacgacggga cacgacgcgg agacgctcat catccgccag aaggacgtgt tcgacgacgc
     2341 gacgccctcg gccaacgcga tggctgccaa cgggttgctg cggctcagcg cgctcactgg
     2401 cgacaatgcg tacgacacgc gcgcccgcgc ctggctcgcg cgcgtcgggc cgaccatggc
     2461 gtcgcacccg acggcgttcg cacattcgct ccaggccctc gaacgcgcag tgacgccgcc
     2521 ggtcgaagtc gtcgtcatcg gcgacccgac gaacgacgac gcccgcgcac tgtggcgcga
     2581 ggtcacggcg cggctccggc ccaacaccgt cgtcgtgcga ggtgccgcac ccgatccggc
     2641 accgacgacg ccgctgctcg aggcgcgcac gctcgtcggc ggtgcgccga cggcgtacgt
     2701 gtgcgaaggc tacgcgtgcc gccaaccggt gacggacgtg caggagctac gcgggcagct
     2761 ggacgccgtg ctgggtcagc aggccgcgac gtagttgccg gccgcgagat cctcggccac
     2821 ggcccaaccg aacgcgatca tcgcctgacc gcgcgcggca tcgacgtcgc cgacgagcga
     2881 gacgatcttc gccccaccgg tcgccttgat cgtgctcgcg tcgaggatcg gcacgcacgg
     2941 cggcttgcga cccttgatcg cggtcttgaa cgtgcgctcg tcgtagccgg cgtcgaggca
     3001 gaaccgcttc gcgaggcggc gaccccacgc gcgatcttcg ccgttgggcg tgtagcccgg
     3061 acgcgggcag acgttcgcgt acgtctgcaa caacgtgtat gcgctcggct gcgccaacct
     3121 cgtcgcgacg aggacgtcct cggcggggaa cgagatcgcg gcccggcgcg acagctcacc
     3181 cgtgagcacc gtccgcgcgg ccccggcgtt cttgcccttc cgggccacac cg
//
LOCUS       DASUGA010000621         2594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_130413, whole genome shotgun sequence.
ACCESSION   DASUGA010000621 DASUGA010000000
VERSION     DASUGA010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130413"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..619
                     /locus_tag="VFX21_12275"
     CDS             35..619
                     /locus_tag="VFX21_12275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX5096790.1"
                     /translation="MLAIALLIPLAWLLGTFPSAGLVARARGVDIAGEGSGNPGASNV
                     ARLLGWKYGALVLLADFAKGALATGVGLAIDGRPGAYVLGAAAVIGHTFPLYRKGGKG
                     VATAGGMLTVLFPWISLGLFLVWVIVSRVANKASLASLLITVLFPLCVAISGYDLWEV
                     AVTGGLAILIIVRHTANIRRLLGRSEIDLTAGPS"
     gene            630..1517
                     /gene="galU"
                     /locus_tag="VFX21_12280"
     CDS             630..1517
                     /gene="galU"
                     /locus_tag="VFX21_12280"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEX5096791.1"
                     /translation="MQTVRKAVIPAAGLGTRFLPATKSSPKEMLPVVDKPAIQYVVEE
                     AVAAGLTDILIVTGRSKRAVEDHFDRNFELEHFLETTGKHQLLKEVQFASDLADVHYA
                     RQRDPLGLGHAVAVARHHVGDEPFAVLLADDIMVDEAGLLSAMLSDFAQHGRSSLGLM
                     EVGPDEISSYGCVDPAGDVDDKGFLQVRRIVEKPPRDEAPSNLAVIGRYVFTPAIFDA
                     LDRIEPGRGGELQLTDAIALLLADEAVYARVCTKGRYDVGQKLDWLRANVELSLDRPD
                     LGPEFAAYLRELVRRRGLA"
     gene            1514..>2594
                     /gene="glp"
                     /locus_tag="VFX21_12285"
     CDS             1514..>2594
                     /gene="glp"
                     /locus_tag="VFX21_12285"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX5096792.1"
                     /translation="MIPLEAAQADVVDAVHALTPVATPLARACGLVLAEEISASENVP
                     PFPNTAMDGYAVRAADTAGAPRRLRVVGELPAGRAPTIAVGAGEAIRIMTGAPMPDGA
                     DAVVMVESTEVDGDEVVINASAQPGEHVRPAGGDVEAGRLVFPPGTALTPAHIGVLAS
                     LDRHEVKCHPRPRVGVVSTGDELVERGALAPGQIRDSNRPMLLALLDASGCEAVDYGI
                     ARDDEAEIEARISQAAGECDALLTSGAVSVGDYDFVKVVLEKLAAQRPGSAFTWSQVA
                     IRPAKPLAFGTLGSVPVFGLPGNPVSSRVSFELFARPALRKLMGCTDLFPAPVAMTAK
                     TAMTRRPDGKLHLDRVRVWVEHGRYV"
BASE COUNT          368 a          902 c          907 g          417 t
ORIGIN      
        1 gggcgcggtc cccgccgcgg cacaatgagc accagtgctg gcgattgcgc ttctcatccc
       61 tctcgcgtgg ctgctcggaa cgttcccgag cgcgggactc gtcgcccgcg cccgcggtgt
      121 cgacatcgcg ggcgaaggat cgggcaatcc gggcgcgtcg aacgttgccc gcctgctcgg
      181 ctggaagtac ggcgcgctcg tcctgctcgc cgacttcgcc aagggtgcgc tcgcgacggg
      241 cgtgggtctc gcgatcgacg gccggcccgg cgcgtacgtg ctcggtgcgg ccgcggtgat
      301 cggccacacg ttcccgctct accgcaaggg cggcaagggt gttgcgacgg ccggcggcat
      361 gctcaccgtc ttgttcccgt ggatctcgct cggcctgttc ctcgtgtggg tgatcgtcag
      421 tcgagtcgcg aacaaggcgt cgctcgcctc gttgctcatc acggtgctct tccccctttg
      481 cgtcgcgatc agtgggtacg acctgtggga ggtcgccgtg accggcggcc tcgcgattct
      541 catcatcgtg cgccatacgg cgaacattcg ccgcttgctc gggcgctccg agatcgacct
      601 cacggccggg ccttcctgaa ggggacaccg tgcaaacagt tcggaaggcc gtcatccccg
      661 ctgccgggct cggcacgcgc ttcttgcccg cgacgaagag ctcgccgaag gagatgctgc
      721 cggtcgtcga caagcctgcg atccaatacg tcgtcgaaga agcggtcgcc gccgggctca
      781 ccgacatcct gatcgtcacc gggcgcagca agcgcgcggt cgaagaccac ttcgaccgca
      841 acttcgagct cgagcacttc ctcgagacga ccggcaagca ccagctgctg aaggaagtgc
      901 agtttgcgtc cgacctcgcg gacgtccact acgcgcgtca acgcgacccg ctcgggctcg
      961 gccacgcggt cgcggtcgcg cgtcaccacg tgggtgacga gccgtttgcg gtgctgctcg
     1021 ccgacgacat catggtcgac gaagccggtc tgctcagcgc gatgctctcc gacttcgcgc
     1081 agcacgggcg ctcctcgctc gggctcatgg aggtcgggcc cgacgagatc tcgtcgtacg
     1141 gatgtgtcga tcccgccggt gacgtcgacg acaaggggtt cctgcaagtg cggcgcatcg
     1201 tcgagaagcc accgcgcgac gaagcgccgt cgaacctcgc ggtcatcggg cgctacgtgt
     1261 tcacgcccgc gatcttcgac gcgctcgacc gcatcgagcc cggccgcggc ggcgagctgc
     1321 aactcaccga cgcgatcgcg ctgttgctcg cagacgaagc cgtgtacgca cgtgtgtgca
     1381 cgaagggccg ctacgacgtc gggcagaagc tcgactggct gcgcgccaac gtggagctct
     1441 cgctcgaccg tcccgatctc ggccctgaat tcgcggcgta cttgcgcgag ctcgtgcgaa
     1501 ggcgcggact cgcgtgatcc cgctcgaagc ggcacaagca gatgtcgtcg acgcggtgca
     1561 cgcgctgacg ccggtcgcga caccgctcgc gcgcgcgtgc gggctcgtgc tcgccgaaga
     1621 gatcagcgcg tccgagaacg tcccgccgtt ccccaacacc gcgatggacg gctatgcagt
     1681 gcgcgcggcc gataccgccg gcgcgccgcg gcggttgcgt gtcgtcggcg agttgcctgc
     1741 gggccgggcg ccgacgatcg ccgtcggcgc gggcgaagcg atccggatca tgaccggcgc
     1801 gccgatgccc gacggcgccg acgcggtcgt gatggtcgag tcgacggagg tcgacggcga
     1861 cgaggtcgtc atcaacgcgt ccgcccagcc cggagagcac gtgcgccccg ccggtggcga
     1921 cgtcgaagcg ggccggctcg tgttcccgcc gggcaccgcg ttgaccccgg cgcacatcgg
     1981 tgtgctcgcg agcctcgacc ggcacgaggt gaagtgccac ccgcggccgc gcgtcggtgt
     2041 ggtgtcgacc ggcgacgagc tcgtcgagcg cggcgcgttg gcgccgggcc agatccgcga
     2101 ctcgaaccgg ccgatgttgc tcgcgctcct cgacgcgagt ggttgcgaag cggtcgacta
     2161 cggcatcgcg cgcgacgacg aagccgagat cgaagcgcgc atctcgcagg ccgccggcga
     2221 gtgcgacgcg ttgctcacga gcggcgcggt atcggtcggc gactacgact tcgtgaaggt
     2281 cgtattggag aagctcgccg cccaacgtcc cggcagcgcg ttcacgtggt cgcaggtcgc
     2341 gatccggcct gcgaagccgc tcgcattcgg gacgctcgga tccgtgccgg tgttcgggtt
     2401 gccgggcaac cccgtgtcgt cgcgcgtgag cttcgagctg ttcgcgcgtc cggccctgcg
     2461 caagctgatg ggttgtaccg atttgttccc ggcgccggtc gccatgacgg cgaagaccgc
     2521 gatgacccgt cgccccgacg gcaagctgca cctcgaccgc gtacgggtgt gggtcgagca
     2581 cggccggtac gtct
//
LOCUS       DASUGA010000622         2177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_199528, whole genome shotgun sequence.
ACCESSION   DASUGA010000622 DASUGA010000000
VERSION     DASUGA010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199528"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VFX21_12290"
     CDS             complement(<1..397)
                     /locus_tag="VFX21_12290"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5096793.1"
                     /translation="MLRPDDSDLEEVEDALVDGDVAYQRGTAQAALRNANFRKVYFGT
                     FASNVGTWMQNVILGAYALKLTGSPAFVGLIFFAQLGPLLFLSTTGGLLADTVDRRRL
                     LVSAQLAQMLLSFGLAAIVLTDEPSRVALV"
     gene            complement(424..>2177)
                     /locus_tag="VFX21_12295"
     CDS             complement(424..>2177)
                     /locus_tag="VFX21_12295"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5096794.1"
                     /translation="VTGAFAALGAVTAWWRASRSGVAEHVDVSMLEAMQQTFVTAPTV
                     MSRFPGGFMGSYRWVMIPGNEPTGDGRFVGITTVTSQQWLSLCRLMGRDDLCDDEELT
                     TMRGRGARAAEVNAILHEWTSKQTADEVVAACVDARVPAAIVGNGAEMPRNEQLAARD
                     VFVTQPGETWIRPRAPFRFHGVADRPLAPPATTDEPWDALAPPRDRAPYAPGERPLAG
                     VRVLDFTAFWAGPFATAWLAAMGADVVKVEAAQRPDGIRFSALLSPRTDPQWYEKSAL
                     FHASNLGKRGITLDLSQPDGLDLARRLVATSDVVVENFTPRVLEQFGLGEETVRRLAP
                     NGIFVRMPAFGLTGPWRNRPGFAQTMEQLTGMAWVTGYEGGPPIIAGGLVDPMVGTHA
                     ALAIVAALEHRARTGEGQLVEVPLVEVATAVTAEQVIRYSADGTLVGRRGSGGVYRCD
                     GDDAWVAVDRTRDPLDADARAAWCATRTADDAARELVASGIPAAAMVPAYLGNEDAQM
                     QARGFFEPVEHPLVGAHEYPTWPMRFSAGPARYWQGPAPTLGQHTDDVLRAVLGLDDA
                     ELDRLREIGVTGTSPRS"
BASE COUNT          322 a          763 c          781 g          311 t
ORIGIN      
        1 ccacgagcgc gacgcgcgac ggctcgtcgg tgagcacgat cgcggcgagt ccgaacgaga
       61 gcagcatctg cgcgagttgc gcgctgacga ggagccggcg gcggtcgacc gtgtcggcca
      121 ggagaccgcc cgtcgtcgac aggaacagca gcgggccgag ctgcgcgaag aagatgaggc
      181 cgacgaacgc gggcgagccc gtcagcttca acgcgtacgc ccccaggatg acgttctgca
      241 tccacgtgcc gacgttcgat gcgaacgtgc cgaagtagac cttgcggaag ttggcgttgc
      301 gcagggcggc ctgcgcagta ccgcgttggt acgcgacgtc gccgtcgacg agagcgtcct
      361 cgacttcttc gagatcggag tcgtcggggc gcaacatcgc ccaaatctac ggcgttcgtg
      421 ccgctacgaa cgaggcgaag taccggtgac gccgatctcg cgcaagcgat ccagctcggc
      481 gtcgtcgaga ccgagcacgg cgcgcagcac gtcgtcggtg tgctggccga gcgtcggcgc
      541 cgggccctgc cagtagcgcg ccgggccggc ggagaagcgc atcggccacg tcgggtactc
      601 gtgcgcaccg accagcggat gctcgacggg ctcgaagaag ccgcgtgcct gcatctgcgc
      661 gtcttcgttg ccgagatacg ccggcaccat cgcggccgcg ggaatgccgg acgcgaccag
      721 ctcgcgggcg gcatcgtccg cggttcgtgt cgcgcaccac gcggcccggg cgtccgcgtc
      781 gagcgggtca cgggtgcggt cgacggccac ccacgcgtcg tcgccgtcgc atcggtacac
      841 accgcccgaa ccgcgtcgac ccaccagggt gccgtcagcc gaatagcgaa tgacctgttc
      901 ggcggtaacg gccgtcgcga cctcgacgag cggtacttcg acgagttggc cttcgcccgt
      961 gcgggcgcgg tgctcgagcg cggccacgat cgccagtgct gcgtgcgtgc cgaccatcgg
     1021 gtcgacgagc ccgcccgcga tgatcggcgg cccgccctcg tagcccgtga cccacgccat
     1081 gccggtgagc tgttccatcg tctgcgcgaa gccgggccgg ttgcgccacg ggccggtgag
     1141 cccgaatgcg ggcatgcgca cgaagatgcc gttcggcgcg agtctgcgga cggtctcctc
     1201 gccgaggccg aactgctcga gcacgcgggg cgtgaagttc tcgacgacga cgtcgctcgt
     1261 cgcgacgagc cgccgggcga gatcgagccc gtcgggttgc gacaggtcga gggtgatgcc
     1321 gcgcttgccg aggttcgacg cgtggaacag ggcggacttc tcgtaccact gcgggtccgt
     1381 gcgcggtgac aggagcgcgc tgaagcggat gccgtccggg cgctgcgccg cttcgacctt
     1441 cacgacgtcc gcgcccatcg ccgccagcca cgcggttgcg aacggaccgg cccagaacgc
     1501 ggtgaagtcg aggacgcgta cgcccgcgag cggccgttcg ccgggcgcgt acggcgcgcg
     1561 gtcccgtggt ggtgcgagcg catcccacgg ctcgtccgtc gtcgcgggcg gtgcgagagg
     1621 gcggtcggca accccgtgga agcggaacgg cgcgcgcggc cggatccacg tctcccccgg
     1681 ttgcgtgacg aacacgtcgc gcgcggccag ttgttcgttg cgcggcatct ccgcgccgtt
     1741 gcccacgatc gcggccggca cgcgtgcgtc gacgcacgcg gcgacgactt cgtcggccgt
     1801 ctgcttcgac gtccactcgt gcaggatcgc gttcacctcg gccgcgcgcg cccctcggcc
     1861 ccgcatcgtc gtcagctctt cgtcgtcgca caggtcgtcg cgacccatga gccggcacag
     1921 cgagagccac tgctggctgg tgacggtcgt gatgccgacg aagcggccgt cgccggtcgg
     1981 ttcgttgccg gggatcatca cccagcggta cgagcccatg aacccgccgg gaaaccgcga
     2041 catgaccgtc ggcgcggtca cgaacgtctg ctgcatcgct tcgagcatcg acacgtcgac
     2101 gtgctctgcg acgcctgaac gcgacgctcg ccaccatgcg gtgaccgcgc ccagtgccgc
     2161 gaacgcaccg gtgacgt
//
LOCUS       DASUGA010000623         6550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1309858, whole genome shotgun sequence.
ACCESSION   DASUGA010000623 DASUGA010000000
VERSION     DASUGA010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6550
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309858"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VFX21_12300"
     CDS             complement(<1..430)
                     /locus_tag="VFX21_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096795.1"
                     /translation="MTSELEGLLDVQAHDLALDRLRHRREALPERAQLDTATRELAVR
                     ERERDEVQRRREEVLREEKRIDDEAQALGARAAEVEQKLYSGTVTSPRELQAMQADVE
                     MLKGHRSDLEDRELEVMEQRETLEGQLAALDANRATLAADI"
     gene            complement(456..2603)
                     /locus_tag="VFX21_12305"
     CDS             complement(456..2603)
                     /locus_tag="VFX21_12305"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX5096796.1"
                     /translation="MKVVKFVVLFVVTIVVGGAALAGAVALLVPAGATFKDTTAYGPL
                     LPAMQKQDQTSYLYDKNGNMLTPLFAEQDRHPISLSEVPQHLIDAVLAIEDRRFYEHH
                     GVDWQGTTRALVENLDAGEVQQGGSTITQQLVKNTMSTHRERDLETKVREAVLAVRLE
                     HEMSKKEILERYLNVIYLGNGAYGIQSASERYFNKKDPKTLTIGEAALLAGLIQSPEA
                     LNPVTHPDRAARRRATVLDAMVDTGKITEEEARIGREEPLPTKATNPEKQRDYFTDEV
                     VRRLMNDDPTVVGDVGEVLGTTQSERYNAVFRGGLHIDTTYDPALQLQAAKAVNDTLP
                     DDVTEFTEAMVVIDNSNGAVRAMVAGRDFKDSQYNLATQANRQTGSSFKGITLATVLM
                     RGYSPKDTVSGGTLHFPLPGEDWDLECSGGSMSLRDATVKSNNCAYARTLISLGPGNS
                     GSDGARDVIAMAKTLGIDTSEMQPVPSLTLGTQLTSPLDMAEAYSVFANEGIHRNPIF
                     VTKITAPDGTVVFQDNPGGARVLPVEIARTETDILQGVIKSGTGTAARLPRPAAGKTG
                     TTNDNADAWFVGYTPQYTAAVWMGNPDGRVPMNNVGGIRVQGGTYPARTWAAFMKPVM
                     EPLPVVEFTAPNEDVWPAAGSISEKDGRKEGRRRAAPAPSESSTTVPGDTVPPSPTEE
                     TVPGATTPPATAVTPVTPVTPVTPAAPAAPASP"
     gene            complement(2637..6017)
                     /locus_tag="VFX21_12310"
     CDS             complement(2637..6017)
                     /locus_tag="VFX21_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5096797.1"
                     /translation="MTTQPTLFEPAGDEGARLGIRRRLGDTLFVEAGAGTGKTAALVD
                     RVVALVRDDGVPIAAIAAITFTERAAAELRDRVRRALMRERAHPRCAAALDDLDAAAI
                     CTLHAFAQRLLTEFPIEAGLPPRVQVRDEIASAVAFDQRWRDFQDRLLDADEHEATVL
                     LALAAGMSLDKLRAVAEAFADNWDQLDRIGAAPELPVLDLTPWIAAMEAVCARTPECI
                     DGDDKMVHKLEECELFARALRSAPDVIEQVRMLRDAPSLRVSRTGRKTNWRCDLDEVR
                     DELVRLDGRRQDVLDRVADAVLRRLAVVLAAFTAEDVALRRAAGELEFHDLLVLARSL
                     LRDSEHGVHARARLRARYQRLLIDEFQDTDPIQIELAVLLASDDADAATRPWSEVAVE
                     PGSLFFVGDPKQSIYRFRRADIATFLTARETLAPAPLLLNRNFRTVEPVLGFVNRVFG
                     DLIQPWPASQPEYRPLEAVRGAPPGGPAVMLLGAEAHADEPDADTLREREAADVAAAV
                     RTAVDEAWPVHDGDGWRAARLGDICVLLPARTSLGFLEQALDDAGVPYRAETSSLVYG
                     SREVRDLLATMRAIDDPTDALMLVAALRTPLFGCGDDDLYEYRVGHGGRLDLTVAPPD
                     TLPPEHPVAEAIRYLGALHEQRTWMSPSELLERIVRERHMLELSVPSQRFRDVARRLR
                     FVVDQARAFTETTRGTVRDYLEWTARQSAEGARVVETVLPETDDDAVRIMTVHGAKGL
                     EFPIVVFSGMTTRAQVRRGGVQVLFPPGGGYEIRLASGVQTDQFELHQPIDEQMSFHE
                     KLRLLYVACTRARDHLVVSVHRKIRSAKSQAARDDDRQRWTHGDLVWEAAVKQGAVFE
                     TLDGAAPAPLVRLAPERVPPPPLDAWTAARDDAFERARRRRFVAATTLAQRPALSADA
                     RTAADPGLAKDARDLELPPWNKGRYGTAIGRAVHAVLQTIDLASGAGLAEAAAAQAAA
                     EGVLGREMTVVALASSALASATVRDAASKAHWRETYVAIPMDGMTLEGYVDLIYRDGD
                     GLVVVDYKTDAVGSDDELAARLDHYRIQGAAYALAVAQATGEPVSRCVFVFCDPEGAR
                     EVTLAGDVLAAALDQVVTLVADERDRPSPFAPSVFAEP"
     gene            complement(6014..>6550)
                     /locus_tag="VFX21_12315"
     CDS             complement(6014..>6550)
                     /locus_tag="VFX21_12315"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX5096798.1"
                     /translation="RAVPVQLTDGRSVRFNGAIDRVDRAATGNLVVFDYKTGSTSSYT
                     GIGDDDPVTAGTHLQLPIYARVARELFAEPGTGAEAYYWFVGKGNDRWIGYEVSPDID
                     AVFDTALQLIVDGIEAGCFVARPAPPGPRPWVDCHFCDPDGLGTADRWREWERKADDP
                     ALVGYFALAGLVPGEAGA"
BASE COUNT          899 a         2304 c         2314 g         1033 t
ORIGIN      
        1 cgatgtcggc cgcgagtgtg gcccggttcg cgtcgagcgc ggcgagctgc ccttcgaggg
       61 tctcccgctg ctccatcacc tcgagctcgc ggtcctcgag atcggaacgg tgccccttca
      121 gcatctcgac gtcggcctgc atcgcctgca gctcgcgcgg cgacgtcacc gtgcccgagt
      181 agagcttctg ttcgacctcg gcggcccggg caccgagcgc ctgtgcttcg tcgtcgatgc
      241 gcttctcctc gcgcagcacc tcttcacgcc ggcgctgcac ctcgtcgcgc tcgcgttcgc
      301 ggaccgcgag ctcgcgcgtg gcagtgtcga gctgcgcgcg ttccggcagt gcctcccggc
      361 gatggcggag ccgatcgagc gcgaggtcgt gcgcctgcac gtcgagcagc ccttcgagct
      421 cgctggtcat gcgcggaatc ctgccgtgcg cgccgtcacg gactcgcggg cgccgccggc
      481 gccgcgggcg tgaccggggt caccggggtc acgggtgtga ccgccgtcgc cggcggggtc
      541 gttgctccgg gcactgtctc ttcggtcggc gatggcggca ccgtatcgcc cggcaccgtc
      601 gtcgaggact ccgaaggggc cggcgcggcc cgccgccgcc cttccttgcg accgtccttc
      661 tcgctgatcg atcccgcggc cggccagacg tcctcgttgg gcgcggtgaa ctccacgacc
      721 ggcaacggct ccatcaccgg cttcatgaag gccgcccacg tgcgcgccgg gtacgtgcca
      781 ccttgcacac ggatcccacc gacgttgttc atcggtaccc gtccgtcggg gttccccatc
      841 cacacggccg ccgtgtactg cggcgtgtag cccacgaacc acgcgtcggc gttgtcgttc
      901 gtcgtgcccg tcttgcccgc cgccggccgc ggcaaccggg cggccgtacc ggtgccgctc
      961 ttgatgacgc cctgcaggat gtcggtctcg gtgcgggcga tctcgaccgg cagcacccgc
     1021 gcgccgccgg gattgtcctg gaacaccacg gtgccgtcgg gggcggtgat cttcgtgacg
     1081 aagatcgggt tgcggtggat gccctcgttc gcgaacaccg agtaggcctc ggccatgtcg
     1141 agcggcgacg tcaactgcgt gccgagcgtc agtgacggga ccggctgcat ctccgacgtg
     1201 tcgatgccca gcgtcttcgc catggcgatg acgtcacgcg caccgtcgga accgctgttg
     1261 cccggaccga gcgagatgag cgtgcgcgcg tatgcgcagt tgttcgactt cacagtcgcg
     1321 tcgcgcaacg acatgctgcc gcccgagcat tcgagatccc agtcttcgcc cggcagcggg
     1381 aagtgcagcg tcccgccgct gacggtgtcc ttgggcgagt agccgcgcat gagcacggtg
     1441 gccagcgtga tgcccttgaa cgacgatccc gtctgccggt tggcctgggt tgccaggttg
     1501 tactgggagt ccttgaagtc gcggccggcg accatcgctc gcactgcgcc gttcgagttg
     1561 tcgatgacga ccatcgcctc ggtgaactct gtgacgtcgt cggggagcgt gtcgttgacc
     1621 gccttcgcgg cctggagctg gagcgcgggg tcgtaggtcg tgtcgatgtg cagcccgccg
     1681 cggaacactg cgttgtaccg ctcgctctgg gtcgtgccga gcacttcgcc gacgtcgccc
     1741 accaccgtcg ggtcgtcgtt catcaagcgg cggacgacct cgtcggtgaa gtagtcgcgc
     1801 tgcttctcgg ggttcgtcgc cttcgtcggc aacggttctt cgcggccgat gcgcgcttct
     1861 tcctcggtga tcttgccggt gtcgaccatc gcgtcgagca cggttgcgcg ccggcgggcg
     1921 gcgcggtcgg gatgggtgac cgggttcagc gcctcgggcg actggatgag tcccgcgagc
     1981 agcgcggcct cgccgatggt cagcgtcttg ggatccttct tgttgaagta gcgctcggac
     2041 gcggactgga tgccgtacgc accgttcccc aagtagatga cgttcaggta tcgctcgagg
     2101 atctccttct tcgacatctc gtgctcgagc cgcaccgcga gcacggcttc gcgcaccttc
     2161 gtctcgaggt cgcgttcgcg gtgcgtgctc atcgtgttct tgacgagctg ctgggtgatc
     2221 gtcgagccac cctgttgcac ctcaccggcg tcgaggttct cgacgagcgc gcgcgtcgtg
     2281 ccttgccagt cgacaccgtg gtgctcgtag aagcgccggt cctcgatcgc gagcacggcg
     2341 tcgatgagat gctgtggcac ttccgacagg ctgatcgggt gacggtcttg ttccgcgaac
     2401 agcggcgtca gcatgttgcc gttcttgtcg tacaggtacg acgtctggtc ttgcttctgc
     2461 atcgccggga gcagcgggcc gtacgcagtg gtgtccttga acgtcgcacc cgccggcacc
     2521 aggagcgcga ccgcgccggc gagggcggca cccccgacga cgatcgtgac aacgaacagg
     2581 acgacgaatt tgaccacttt cacggcgtta ccaggatggc acagccggcc accggttcag
     2641 ggttcggcga agaccgacgg cgcgaagggt gacgggcgat cgcgctcgtc ggccacgagc
     2701 gtgacgacct ggtcgagcgc ggcggccagc acgtcaccgg cgagcgtcac ctcgcgggcc
     2761 ccctccgggt cgcagaagac gaacacgcaa cgcgacaccg gctcgccggt cgcctgcgcg
     2821 actgcgaggg cgtaggccgc gccctggatg cggtagtggt cgagccgcgc cgcgagctcg
     2881 tcgtcgctgc cgaccgcgtc ggtcttgtag tcgacgacga cgagcccgtc gccgtcgcgg
     2941 tagatgaggt cgacgtagcc ctcgagcgtc atgccgtcca tcgggatcgc gacgtacgtc
     3001 tcgcgccagt gcgccttcga tgccgcatcg cgcacagtcg cgctggcgag cgccgacgac
     3061 gcgagcgcga ccaccgtcat ctcccgaccc agcacaccct ccgccgcggc ctgcgcggcg
     3121 gcggcctccg cgagacccgc gcccgacgcg aggtcgatcg tctgcaagac cgcgtgcacc
     3181 gcgcgcccga tcgcggtgcc gtaacgaccc ttgttccacg gcggcagctc gagatcgcgc
     3241 gcgtccttcg cgagcccggg atcggccgcc gtccgcgcgt cggccgagag cgccggccgc
     3301 tgcgcaaggg tcgtcgcggc gacgaagcgc cgccggcgcg cccgttcgaa cgcgtcgtcg
     3361 cgtgccgcgg tccacgcgtc gagcggcggc ggcggcacgc gttcgggcgc gagacgcacc
     3421 aacggcgccg gtgccgcgcc gtcgagcgtc tcgaagacgg cgccctgctt caccgcggct
     3481 tcccagacga gatcgccgtg cgtccaccgc tgccggtcgt cgtcgcgcgc cgcctgcgac
     3541 ttcgcggatc gaatcttgcg gtgcaccgag acgacgaggt gatcgcgggc ccgcgtgcac
     3601 gcgacataga ggaggcggag cttctcgtgg aagctcatct gctcgtcgat cggttggtgc
     3661 agctcgaact ggtcggtctg cacacccgag gcgaggcgga tctcgtagcc gccgcccggc
     3721 gggaacagca cttgcacgcc gccgcggcgc acctgagccc gggtcgtcat gccggagaag
     3781 acgacgatcg ggaactcgag gcccttggcg ccgtgcacgg tcatgatgcg gaccgcgtcg
     3841 tcgtcggtct cgggtagcac ggtctcgacg acgcgtgcgc cctccgcgct ctggcgggcg
     3901 gtccattcga ggtagtcgcg caccgtgcct ctcgtcgtct cggtgaaggc gcgcgcctgg
     3961 tcgacgacga accgcagccg gcgggcgacg tcgcggaagc gttgtgacgg gacgctcaac
     4021 tcgagcatgt ggcgctcgcg cacgatgcgc tcgagcagtt cgctcggcga catccaggtg
     4081 cgctgttcgt gcaacgcgcc gagatagcgg atcgcctccg cgaccggatg ctccggcggc
     4141 aatgtgtcgg gcggcgcaac ggtgaggtcg agtcggccgc cgtgcccgac gcggtactcg
     4201 tagagatcgt cgtcgccaca gccgaacaac ggcgtgcgca gcgcggcgac gagcatgagt
     4261 gcatcggtcg gatcgtcgat cgcgcgcatt gtcgcgagga ggtcgcgcac ctcacggctg
     4321 ccgtagacga gcgagctcgt ctcggcgcga tacggcacgc cggcgtcgtc gagggcctgc
     4381 tcgaggaagc cgagcgacgt ccgcgccggc aatagaacac agatgtcacc cagtcgcgcc
     4441 gcgcgccatc cgtcgccgtc gtgcacgggc cacgcttcgt cgacggcggt acgcaccgcg
     4501 gccgcgacgt cggccgcctc gcgttcgcgc aacgtgtcgg cgtcgggctc gtcggcgtgt
     4561 gcctcggcac cgagcaacat caccgccggc ccgccgggcg gcgcgccgcg cacggcctcg
     4621 agcgggcggt actcgggctg cgacgccggc cacggctgga tcaagtcgcc gaacacgcgg
     4681 ttgacgaacc cgagcaccgg ctcgactgta cggaagttgc ggttcaggag caggggtgcc
     4741 ggcgcgagcg tctcgcgcgc ggtcaggaat gtcgcgatgt cggcgcgccg gaagcggtag
     4801 atcgactgct tcgggtcacc gacgaagaac aaggagcccg gttcgaccgc gacctcggac
     4861 cacggacgcg tggccgcgtc cgcgtcgtcg gacgcgagga gcactgccag ctcgatctgg
     4921 atcgggtcgg tgtcctggaa ctcgtcgatg aggagccgtt ggtagcgcgc ccgcagccgc
     4981 gcccgcgcgt ggaccccgtg ctccgaatcg cgcagcagtg accgggccag cacgagcaga
     5041 tcgtggaact ccagctcgcc cgcggcgcgc cgcagcgcga cgtcttcggc cgtgaacgcg
     5101 gccagcacca ccgccaagcg acgcagcacc gcgtcggcga cccggtcgag cacgtcttgg
     5161 cgtctcccgt cgagacgcac cagctcgtcg cgcacctcgt cgaggtcgca gcgccagttc
     5221 gtcttgcgcc ccgtacgcga gacgcgcaac gacggcgcgt ccctcagcat gcggacctgc
     5281 tcgatgacgt cgggcgccga gcgcagcgct cgcgcgaaga gctcgcactc ctcgagcttg
     5341 tgcaccatct tgtcgtcgcc gtcgatgcat tccggcgtgc gcgcgcagac cgcctccatt
     5401 gcggcgatcc acggcgtgag atcgaggacc ggcagctcgg gtgcggcgcc gatgcggtcg
     5461 agttggtccc agttgtcggc gaacgcctcc gcgacggcgc gcagcttgtc gagcgacatg
     5521 ccggccgcga gggcgagcag caccgtggct tcgtgctcat cggcatcgag gagccggtcc
     5581 tggaagtcgc gccagcgctg gtcgaacgcg accgccgacg cgatctcgtc gcggacctgc
     5641 acccgcggcg gcagtccggc ctcgatcggg aactccgtca gcagtcgttg tgcgaacgcg
     5701 tgcaacgtgc agatcgcggc cgcgtcgagg tcgtcgagcg cggccgcaca ccgcggatgc
     5761 gcgcgctccc gcatcagtgc gcgacggacg cggtcgcgca actcggccgc ggcccgttcg
     5821 gtgaacgtga tggcggcgat cgcggcgatc gggacgccgt cgtcgcgcac caacgcgacg
     5881 acacgatcga cgagcgcggc ggtcttgccc gtgcccgcgc ccgcctcgac gaacaacgtg
     5941 tcgcccaggc ggcgccggat gccgagacgc gcgccctcgt cgccggcggg ctcgaacagt
     6001 gtcggctgcg tcgtcatgcg ccggcctcgc ccgggaccag gccggcgagc gcgaagtagc
     6061 cgacgagcgc gggatcgtcg gccttgcgct cccattcgcg ccaccggtcg gcggtgccga
     6121 ggccgtcggg gtcgcagaag tggcagtcga cccacggccg cggccccggc ggcgcgggcc
     6181 gggcgacgaa gcaaccggct tcgatgccgt cgacgatgag ctggagggcc gtatcgaaca
     6241 ccgcgtcgat gtcggggctc acctcgtacc cgatccagcg gtcgttgccc ttgccgacga
     6301 accagtaata cgcctccgcg ccggtgccgg gctctgcgaa cagctcacgc gccacacgcg
     6361 cgtagatcgg cagctgcaga tgagtgcccg cggtcaccgg atcgtcgtcg ccgatgccgg
     6421 tgtacgacga cgtcgagccg gtcttgtagt cgaacacgac gagattgccg gtcgcggcgc
     6481 gatcgacgcg gtcgatcgcg ccgttgaagc ggaccgaccg gccgtcggtg agctgcaccg
     6541 gcacggcgcg
//
LOCUS       DASUGA010000624         4089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1135691, whole genome shotgun sequence.
ACCESSION   DASUGA010000624 DASUGA010000000
VERSION     DASUGA010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135691"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      193..295
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            371..1606
                     /locus_tag="VFX21_12320"
     CDS             371..1606
                     /locus_tag="VFX21_12320"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX5096799.1"
                     /translation="MVYVNGLRCRECGREYRADPIYTCEWCFGPLEVAYDYDTIAKEA
                     SREKIAAGPPSLWRYADLLPVAGDTAVDLGTGFTPLVRADRLAAELGLREVWIKNDTR
                     NPTNSFKDRVVSIALSKALEFGFKVAACASTGNLANSVAAHAARAGLRSYVFIPSNLE
                     QGKIVTTAVYGGNVVAINGNYDDVNRLCAELAGIYEWAFVNVNMRPYYAEGSKTLAFE
                     TAEQLGWRSPDHVVVPVASGSLLTKIQKGFQELYKVGLLDEEPDVRVSGAQALGCSPV
                     ATAWLESSDTIRPVKPDTIAKSLAIGNPADGYFALDTVRTTGGGFAAVTDDEVVEGMR
                     LLARTEGIFAETAGGVTVATLQKLAADGVVRPDERVVVYVTGHGLKTLEAVAPTSGPT
                     AVIAPTLDAFHEAFDVEEN"
     gene            1610..1882
                     /locus_tag="VFX21_12325"
     CDS             1610..1882
                     /locus_tag="VFX21_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX5096800.1"
                     /translation="MATVRIPTQLRNLSGGAGEVQLDGGSVAEVLKNLDDQHNGFAER
                     LFDDSGALRRFVNVFVDDEDIRFLQALDTEVTDKTVVSIVPAVAGG"
     gene            1879..2436
                     /locus_tag="VFX21_12330"
     CDS             1879..2436
                     /locus_tag="VFX21_12330"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5096801.1"
                     /translation="MSRCPTLETARLLMRPFRESDLDDYQRVLSAAPVRKSLHLPERI
                     TREDVWQQMAMFLGQWELRNHGQWALEEKATGEFVGRAGPHRPERADWPGIEVGWTLH
                     PEHWGKGYATEAGRAAVDWVFMNHREVDTLYSIILPENGASQAVAQRLGYELSEERVL
                     AFFPTKAHGIWTLPRERWAQRQTAT"
     gene            complement(2421..3068)
                     /locus_tag="VFX21_12335"
     CDS             complement(2421..3068)
                     /locus_tag="VFX21_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017572487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX5096802.1"
                     /translation="MHDTTEEYLETILSIEEEGIVPLRARLVERLGLSAAAVSETVSR
                     LSDQGYVNLRDDRSLELTKKGRSLATTIVRRHRLAERLLVDIIGLEWEKVHREADRWE
                     HAISADVEEKLVELLGDPATCPHGNPIPGSKRATRPGQSVALSAAAPGEVTVTRISEK
                     LELSDDGLHLIALAKLMPGCNATVVERDRDGVHVKTATGEHIVPATVAEQLYVAV"
     gene            3101..3775
                     /locus_tag="VFX21_12340"
     CDS             3101..3775
                     /locus_tag="VFX21_12340"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5096803.1"
                     /translation="MTISRADVVVLGAGPAGLGAAYLLARQGRSVCVLERAPVVGGLA
                     ASFEVAGVRVDHGYAELLDDRSLRLTDEGREIATSIVRRHRLAERLLVDIIGLEWEKV
                     HKEADRWEHAISADVEEKLVQLLGDPATCPHGNPIPGSAHKVEGPASVPLTQMQPGPV
                     IVRRISEKAEIDDDAIAFLAGAELIPGSAAVVVSTSTDGVQVTSAAGEQTVPRNLAQL
                     IYAIPA"
     gene            complement(3847..4086)
                     /locus_tag="VFX21_12345"
     CDS             complement(3847..4086)
                     /locus_tag="VFX21_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012513164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX5096804.1"
                     /translation="MSGGAAEVTAEGGSLASVLEGLETAHPGFRERLFDEQGELRRFV
                     NVFVDDEDIRFLDGVGTEVHDGATVSIVPAVAGGS"
BASE COUNT          659 a         1411 c         1400 g          619 t
ORIGIN      
        1 cggtcgagtt gtcgtcgatc acgatcactt cggcgacgag atcgacgcgc cgcgcctcgt
       61 gcacgatcgg gccgaccgtt tcgccctcat tgcgcgccgg caggcagacg gagacgaagc
      121 caccggaact gcgggcacct gcatcgacca cgaggagcag gctaggggtg ctgctatcct
      181 ctgccgtcaa cgctctcatc cagagcggcc gagggacagg ccctgtgacg ccgcggcaac
      241 cagctcgttc cgggagaccg ggggagccag gtgccaatgc ctgagtcgat gagcagccga
      301 agagcggtcc gtccgctcga ggcgctcgag agcgcgtcga gaacctacgg agaacgagga
      361 gaccgctcgc atggtgtacg tcaatgggct tcgttgccgg gagtgtggcc gcgagtaccg
      421 ggccgatccg atctatacgt gcgagtggtg cttcggtccg ctcgaggtcg cgtacgacta
      481 cgacacgatc gccaaggagg cgagccgcga gaagatcgcg gcgggcccgc cgtcactgtg
      541 gcgctacgcc gacctgctgc ccgtcgccgg tgacaccgca gtcgacctcg gcaccggctt
      601 caccccgctc gtgcgtgccg accgcctcgc ggccgagctg ggactgcgcg aggtctggat
      661 caagaacgac acgcgcaacc cgacgaactc cttcaaggac cgcgtcgtct cgatcgcact
      721 gagcaaggcg ctcgagttcg gcttcaaggt cgcggcctgt gcctcgaccg gcaacctcgc
      781 gaactcggtc gccgcgcacg cggcacgcgc cggattgcgg tcgtacgtct tcatcccgtc
      841 gaacctcgaa cagggcaaga tcgtcacgac cgcggtgtac ggcggcaacg tcgtcgcgat
      901 caacggcaac tacgacgacg tcaaccggtt gtgcgccgag ctcgcaggca tctacgaatg
      961 ggcgttcgtc aacgtcaaca tgcgtccgta ctacgccgaa ggctcgaaga ccctcgcgtt
     1021 cgagaccgcg gagcaactcg ggtggcgttc gcccgatcat gtcgtcgtgc cggtggcgag
     1081 cgggtcgctg ctgacgaaga tccaaaaggg cttccaggag ctctacaagg tcggcttgct
     1141 cgacgaggag cccgacgtgc gcgtctcggg tgcgcaggcg ctcggttgct cgccggtcgc
     1201 gaccgcgtgg ctggagtcct ccgacacgat ccgccccgtg aagcccgaca cgatcgcgaa
     1261 gtcgctcgcg atcgggaacc cggccgacgg ctacttcgcg ctcgacaccg tgcgcaccac
     1321 cggcggcggc ttcgcggccg tgaccgacga cgaagtggtg gaggggatgc ggttgctggc
     1381 ccgcaccgaa gggatcttcg cggagaccgc cgggggcgtg acggtcgcga cgttgcagaa
     1441 gctcgcggcc gacggcgtcg tccgccccga cgagcgagtc gtcgtctacg tcaccgggca
     1501 cggcctgaag acactcgaag ccgtcgcgcc cacgagcggc cccaccgcgg tcattgcacc
     1561 tacgctggac gcattccacg aagccttcga cgtggaggag aactgagcaa tggcaaccgt
     1621 tcgtatcccc acgcagctgc gcaacctctc gggtggcgca ggtgaggtgc agctcgacgg
     1681 cgggagcgtc gccgaggtgc tcaagaacct cgacgaccag cacaacggct tcgcggagcg
     1741 tctcttcgac gacagcggcg cactgcgtcg cttcgtgaac gtcttcgtcg acgacgaaga
     1801 catccgtttc ctgcaggccc tcgacaccga agtgaccgac aagacggtcg tcagcatcgt
     1861 cccggcggtc gccggcgggt gagccgttgc ccgacgctcg aaacggcgcg cctcctgatg
     1921 cgcccgtttc gcgagtccga tctcgacgac taccaacgcg tgctgtcggc ggcgccggtg
     1981 cgcaagtcgt tgcatcttcc ggagcgcatc acgcgcgagg acgtgtggca gcagatggcg
     2041 atgttcctcg gccagtggga gctgcgcaat cacggtcagt gggcgctcga ggagaaggcg
     2101 acgggggagt tcgtcggccg cgccggcccc caccgtcccg aacgcgccga ctggcccggc
     2161 atcgaagtcg ggtggacgct gcatcccgag cactggggca agggctacgc gaccgaggcc
     2221 ggtcgcgccg cggtcgactg ggtgttcatg aaccaccgcg aggtcgacac gctctacagc
     2281 atcatcctgc cggagaacgg tgcgtcacag gccgtcgcgc aacggctcgg ctacgagctc
     2341 tccgaggaac gcgtgctcgc gttcttcccg acgaaagcgc acggcatctg gacactgccg
     2401 cgcgagcgct gggcgcagcg tcagaccgcg acgtagagct gctcggcgac cgtcgccggc
     2461 acgatgtgct cgcccgtcgc cgtcttcacg tgcacgccgt cgcgatcgcg ctcgacgacc
     2521 gtcgcgttgc agccgggcat caacttcgcg agcgcgatga ggtgcaagcc gtcgtcggag
     2581 agctcgagct tctcgctgat gcgcgtcacg gtcacctcac cgggcgccgc ggccgagagc
     2641 gccaccgact gaccgggtcg cgtcgcgcgc ttcgaaccgg ggatcgggtt gccgtgcggg
     2701 cacgttgcgg ggtcgccgag cagctcgacc agcttctcct cgacgtcggc ggagatcgcg
     2761 tgctcccagc ggtcggcctc gcggtgcacc ttctcccact cgagcccgat gatgtcgacg
     2821 agcaggcgct cggcgagacg gtggcggcgc acgatcgtgg tcgcgaggct gcggcccttc
     2881 ttcgtgagct cgaggctgcg gtcgtcgcgc agattgacgt agccctgatc cgacaagcgc
     2941 gacaccgttt cggagaccgc ggccgccgag aggccgagcc gttcgaccaa ccgcgcgcgc
     3001 aacgggacga tgccttcctc ttcgatcgag aggatcgtct cgaggtactc ctcggtggtg
     3061 tcgtgcatct ccgccacgag gccaaagata ccctacggcc gtgacgattt cgcgggccga
     3121 tgtcgtcgtg ctcggcgccg gtcccgccgg tctcggcgcc gcgtatctgc tcgcacgaca
     3181 agggcgatcc gtgtgtgtgt tggaacgcgc gcccgtcgtg ggtggcctcg ccgcgagctt
     3241 cgaggtcgcg ggcgtgcgcg tcgaccacgg ctacgccgag ctgctcgacg atcgcagcct
     3301 gcgcctcacc gacgagggcc gcgagatcgc gacgtcgatc gtgcgccggc accgcctcgc
     3361 ggagcggctg ctcgtcgaca tcatcgggct cgagtgggag aaggtccaca aggaggccga
     3421 ccgctgggag cacgcgatct cggccgacgt ggaggagaag ctcgtgcagc tcctcggcga
     3481 tccggccacg tgcccccacg ggaacccgat ccccggttcc gcccacaagg tcgagggacc
     3541 cgcgtcggtc cccctcaccc agatgcagcc cggaccggtg atcgtccgcc gcatcagcga
     3601 gaaggcggag atcgacgacg acgccatcgc gttcctcgcc ggcgccgagc tgatccccgg
     3661 ttcggccgcg gtggtcgtca gcaccagcac cgatggcgtg caggtcacca gcgccgcggg
     3721 cgagcagacc gtcccccgca acctcgcgca gctgatctac gccatccctg cctgaacgaa
     3781 cgggtttcgg cgtcgaagtt gcggccttat ggcgcaacgt cgacgcatgt tcgtggagac
     3841 ggcgcgctag ctcccgccgg cgacggcggg gacgatgctc acggtggcac cgtcgtgcac
     3901 ctcggtgccg accccgtcga ggaagcggat gtcctcgtcg tcgacgaaca cgttcacgaa
     3961 gcggcgcagc tcgccctgtt cgtcgaagag gcgctcccgg aatccggggt gcgcggtctc
     4021 caatccctcc aggaccgacg cgagcgagcc gccctcggcg gtgacctcgg cggcgccgcc
     4081 ggacagctc
//
LOCUS       DASUGA010000625         2969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1607369, whole genome shotgun sequence.
ACCESSION   DASUGA010000625 DASUGA010000000
VERSION     DASUGA010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607369"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VFX21_12350"
     CDS             <1..628
                     /locus_tag="VFX21_12350"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5096805.1"
                     /translation="VCGLGLGWFESEHTALGWPFPSVDERYALLEDALEFLPIFWGKG
                     TPAYAGRVLEVPEAMCYPRPLQEHIPLLVGGSGEKRTLRLVAKYADACNIIGEVDVVR
                     RKVRVLHDHCLAIGRDPETIAITQLSTTLIGRTGIDIAAQVERYKPRRVSSESYAKRV
                     NAGTPGDQIGRFRALAEAGVQHAIVSFPDLSNNEPIEYFAKVIAAFAE"
     gene            complement(682..1479)
                     /locus_tag="VFX21_12355"
     CDS             complement(682..1479)
                     /locus_tag="VFX21_12355"
                     /inference="COORDINATES: protein motif:HMM:NF019599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1775 domain-containing protein"
                     /protein_id="HEX5096806.1"
                     /translation="MKRIFGATLFAFVLIAGLAAPAFAHSEITPNSVEAGSTVPFSLF
                     VEDEKSDANTVKVELTFPQPLVVSDLPAAAGLTATVVGGSVGAEATGITWDGASESDV
                     NVPFSLVVPDEAGRLQFKVLQTYDTGDVDRWIQEWPAGAPEPDNPGPVVDVVAAGTPT
                     SQAGQSAEEHAAEHAAEGDHDEATSAEEHAAEHAENGDHDEATTTSVAAEDDSDSDDS
                     NTGTIILVAVVVVLLVGGGIAFFVMRKKPSGGAPPPSSDDTPTPTSA"
     gene            complement(1522..2697)
                     /locus_tag="VFX21_12360"
     CDS             complement(1522..2697)
                     /locus_tag="VFX21_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5096807.1"
                     /translation="MTRHPTRPLVVARGIRAFTDGFAGVLLARYLQNLGFNGFQIGVI
                     VTATLLGSAALTLWAGLRLGRWAARTVLFASCGLMAFTGAGFATVTRFWPLVAIGFAG
                     TLNPSGGDVSLFLPTEQAVIADLSEERKRPHRFAVYNLAAAFAAAGGAAFSALPDRIA
                     RNNGWDVVTVERLSFLVYVVAAIACAFVYRSLRTHAHLTGNRGRLHKSRNIVMRLSAL
                     FSLDSAGGGFVVPALLVLYLDVRFDLSPQRMAATFAAAGVLTGVSQLAAARLAGRIGH
                     VHTMVFTHLPANVFLILAGVVPNAAAAVTLLLMRAALSQMDVPVRQSLVMRVVEPDER
                     AAAASITNVPRSLASALTPALGGLLLTHSTFGWPLIIGGTMKGIYDVLLLAQPLDRD"
     gene            complement(2694..>2969)
                     /locus_tag="VFX21_12365"
     CDS             complement(2694..>2969)
                     /locus_tag="VFX21_12365"
                     /inference="COORDINATES: protein motif:HMM:NF037864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX5096808.1"
                     /translation="GEVMVDGNRRDATVERTVIDTIECAVLVGHEGELYEGVVVDRHK
                     DGVVVQLTEPAVVMNVAVEAPLGSEIALRLEHVDPVARRTTFVPSRT"
BASE COUNT          482 a         1023 c         1040 g          424 t
ORIGIN      
        1 ggtgtgcggg ctcgggctcg gctggttcga gtccgagcac acggcgttgg ggtggccgtt
       61 tccgtcggtc gacgagcggt acgcgctgct cgaggacgcg ctcgagttcc tgccgatctt
      121 ctggggcaag ggcacgcccg cgtacgccgg ccgcgtactc gaagtgcccg aggcgatgtg
      181 ctacccgcgg ccgctgcagg agcacatccc cctcctcgtc ggcggcagcg gcgagaagcg
      241 gacgttgcgg ctcgtcgcca agtacgcgga cgcgtgcaac atcatcggtg aagtcgacgt
      301 ggtccgccgc aaggtgcggg tgctgcacga ccactgcctc gcgatcggtc gtgacccgga
      361 gacgattgcg atcacgcagt tgtcgacgac gttgatcggt cgcacgggca tcgacatcgc
      421 cgcgcaggtc gagcggtaca agccgcggcg cgtcagctcg gagtcatacg cgaagcgggt
      481 gaacgcgggg acgcccggcg accagatcgg tcgcttccgc gcgctcgcgg aggcaggcgt
      541 gcagcacgcg atcgtcagct tccccgacct gtcgaacaac gaaccgatcg agtacttcgc
      601 gaaggtgatc gccgccttcg ccgaatgagg acgggcgtct tacaagggcg aattccgccc
      661 ttgtaagacg cccgaccgtc gtcaggccga agtgggggtg ggggtgtcgt cgctcgacgg
      721 cggcggagcg ccgccgctcg gcttcttgcg catcacgaag aaggcgatgc cgccgccgac
      781 gagcagcacg acgacgactg caacgaggat gatcgtgccg gtgttcgagt cgtcgctgtc
      841 gctgtcgtcc tccgccgcga cggacgtcgt cgtcgcttcg tcgtggtcgc cgttctcggc
      901 gtgctcggct gcatgctcct ctgcggacgt tgcttcgtcg tgatcacctt cagccgcgtg
      961 ctccgccgcg tgctcctcgg ccgactgccc cgcttggctc gtcggcgtgc ccgcggcgac
     1021 gacgtcgacg acggggcccg ggttgtcggg ttcgggcgcg cccgcgggcc actcctggat
     1081 ccaccggtcg acatcaccgg tgtcgtacgt ctgcaacacc ttgaactgga gccgccccgc
     1141 ctcgtcgggg acgacgagcg agaacggcac gttcacgtcg ctctcgctcg cgccgtccca
     1201 cgtgatgccc gtcgcctccg cgcccaccga cccgccgacg acggtcgcgg tcaaaccggc
     1261 ggccgcgggc agatcggaga ccaccagcgg ctgcgggaac gtcagctcca ccttgaccgt
     1321 gttcgcgtcg gatttctcgt cctcgacgaa gagcgagaac gggaccgtgc tccccgcctc
     1381 gaccgagttc ggtgtgatct cgctgtgggc gaacgcgggc gcggcgaggc ccgcgatcag
     1441 gacaaaggcg aacagagtcg cgccgaagat gcgtttcatg ctgcgacagt cgcccgggcc
     1501 gcccgatcag ttccacgacc actaatcgcg gtcgaggggc tgcgcgagaa ggagcacgtc
     1561 gtagatgccc ttcatcgtgc cgccgatgat cagcggccaa ccgaaggtcg agtgcgtgag
     1621 cagcaaaccg ccgagcgcgg gcgtcagcgc cgaggccagg ctgcggggca cgttcgtgat
     1681 gctcgcggca gccgcccgct cgtcgggttc gacgacgcgc atgacgagcg attgccgcac
     1741 gggcacgtcc atctgcgaga gcgcggcgcg catcagcaac agcgtcacgg ccgcggccgc
     1801 gttggggacg acgccggcga ggatcaagaa gacattcgcg ggcagatgcg tgaacaccat
     1861 ggtgtgcacg tgcccgatcc ggccggcgag gcgcgcggcg gcgagttgcg agacgcccgt
     1921 cagcacgccc gcggccgcga acgtcgcggc catcctctgc ggcgacagat cgaagcgcac
     1981 gtcgagatac agcacgagca gcgcggggac gacgaacccg ccgcccgccg agtcgaggga
     2041 gaacagcgcc gagaggcgca tcacgatgtt gcgcgacttg tgcagccggc cgcggttccc
     2101 ggtcaggtgc gcgtgcgtgc gcagcgagcg gtacacgaac gcgcacgcga tcgcggcgac
     2161 aacgtagacg aggaacgaca agcgctcgac ggtgacgacg tcccaaccgt tgttccgggc
     2221 gatgcgatcg ggaagcgcgg agaacgccgc gccgccggcc gccgcgaacg cggccgcgag
     2281 gttatagacc gcgaatcggt gcggacgctt gcgctcctcg ctgaggtcgg cgatcaccgc
     2341 ctgctcggtg ggcaggaaca agctcacgtc gccgccggac ggattgagcg tgccggcgaa
     2401 tccgatcgcg accagcggcc agaagcgggt gaccgtcgcg aacccggcgc cggtgaaggc
     2461 catcagccca cacgacgcga acagcacggt gcgggccgcc caccggccga ggcgcagccc
     2521 ggcccacaga gtcagggcgg ccgagccgag cagcgtcgca gtgacgatga caccgatctg
     2581 gaacccgttg aacccgaggt tctgcaggta gcgcgcgagc agcacgcccg cgaagccgtc
     2641 ggtgaacgcg cgtatcccac gcgcgaccac cagtgggcgc gtgggatgcc gggtcacgtc
     2701 cggctcggca cgaacgtcgt ccggcgcgcg accggatcga cgtgttcgag acggagcgct
     2761 atctccgatc ccagcggcgc ctccaccgca acgttcatca cgaccgcggg ctccgtcagc
     2821 tgcacgacga caccgtcctt gtgccggtcg acgacgacac cttcgtacag ctcgccctcg
     2881 tgcccgacga gcaccgcgca ttcgatcgtg tcgatcaccg ttcgctcgac cgtcgcgtcg
     2941 cggcgattgc cgtcgaccat cacctcgcc
//
LOCUS       DASUGA010000626         2095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1866327, whole genome shotgun sequence.
ACCESSION   DASUGA010000626 DASUGA010000000
VERSION     DASUGA010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1866327"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1192)
                     /locus_tag="VFX21_12370"
     CDS             complement(44..1192)
                     /locus_tag="VFX21_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX5096809.1"
                     /translation="MAMDVAIIGVGLHPFGRFAGVSGIEMGAVAIRKALEDAGVEWRD
                     IQFAFGGSYEVDNPDAVVALMGLTGIPFTDVYNGCATAASALTLAANTIRLGDSELGL
                     AVGMDKHLPGAFSGDPQDYACPPWYGELGHFITTKFFGMKINKYMHDHGISHQTLARV
                     AAKNYRNGSLNPNAFRRKPLSEEEILGSPMLNYPLTQYMFCSPDEGAAAVVLCPADKA
                     HRYTDNPIYLRATTVRTRRLGAFEVHSPWLPVDDTVGATVVASRAAYEMAGIGPEDVD
                     VIQLQDTDAGAELIHLAENGFCADGDQEALYADGATEIDGPLPVNTDGGLIANGEPIG
                     ASGLRQVHEIVLQLRGRAGDRQVPGNPQVGYTQLYGAPGTAGVSILSR"
     gene            complement(1206..1640)
                     /locus_tag="VFX21_12375"
     CDS             complement(1206..1640)
                     /locus_tag="VFX21_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX5096810.1"
                     /translation="MTHMSGERAISEGLFTWPSDAPQLIGSKCAQCGIVTFPAQGSCP
                     RDASTEMEDHLLPRRGRLWAWTTQDFQPPSPPYTGPKGEDYSRFAVGYVELPGEVKVE
                     SRLTESDPEVLEQGMEMELVVVPFRTDDDGNEVVTFAFRPVD"
     gene            complement(1637..>2095)
                     /locus_tag="VFX21_12380"
     CDS             complement(1637..>2095)
                     /locus_tag="VFX21_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEX5096811.1"
                     /translation="GYLDDPDATAAAFDDEGFLHTGDLGTIDEDGYVRIVGRIKDMFI
                     VGGFNAYPAEIENLLLRHPRISQAAVIGVPDARLGEVGMAFVVLDGNGPPLDTDEVIG
                     WSRDEMANFKVPRFVEFVDELPINATGKVMKDELRARAQRANRVTGDKET"
BASE COUNT          300 a          747 c          697 g          351 t
ORIGIN      
        1 gatcggtcgg cgtgtacgtc acggcggtca ccgcgtcagc acctcaccgc gacaggatcg
       61 acacgccggc ggttccgggc gcgccgtaca gctgcgtgta accgacctgc gggttcccgg
      121 gcacctgccg gtcgcccgcc cggccgcgca gctgcagaac gatctcgtgc acctggcgca
      181 gtcccgaggc gccgatcggc tcgccgttgg cgatcagccc gccgtcggtg ttcaccggca
      241 acgggccgtc gatctccgtc gcgccgtcgg cgtacagcgc ctcctggtcg ccgtcggcgc
      301 agaagccgtt ctcggcgagg tggatgagct cggcgccggc atcggtgtcc tgcaactgga
      361 tgacgtcgac gtcctcgggc ccgatgcccg ccatctcgta cgcggcgcgc gacgcgacga
      421 cggtcgcccc cacggtgtcg tcgaccggca gccacgggct gtgcacctcg aacgcgccca
      481 gccgccgcgt gcgcacggtc gtcgcgcgca agtagatcgg gttgtcggtg tagcggtgcg
      541 ccttgtccgc cgggcacagc acgaccgccg ccgcgccttc gtcggggctg cagaacatgt
      601 actgcgtgag tgggtagttg agcatcggcg agccgaggat ctcctcctcc gacagcggct
      661 tgcggcggaa cgcgttcggg ttcagcgagc cgttgcggta gttcttcgct gcgacgcgtg
      721 cgagcgtctg gtgcgagatg ccgtggtcgt gcatgtactt gttgatcttc ataccgaaga
      781 acttcgtggt gatgaagtga ccgagctcgc cgtaccacgg cgggcacgca tagtcctgtg
      841 ggtcgccgct gaacgcgccc ggcaggtgct tgtccatgcc gacggcgagc ccgagctcgc
      901 tgtcgccgag ccggatcgtg ttcgcggcca gcgtgagcgc gctcgcggcg gtcgcgcagc
      961 cgttgtagac gtcggtgaac ggaatgcccg tgagccccat cagcgcgacc acggcatcgg
     1021 ggttgtcgac ctcatagctg ccaccgaacg cgaactggat gtcgcgccac tccacgcccg
     1081 cgtcctcgag cgccttgcgg atcgcgaccg cgcccatctc gatccccgac acaccggcga
     1141 agcggccgaa cgggtgcagg ccgacgccga tgatcgccac atccatcgcc atcgcgaacc
     1201 tcgcctcagt cgacgggtcg gaacgcgaac gtcacgacct cgttgccgtc gtcgtcggtg
     1261 cggaacggca ccaccacgag ctccatctcc atcccctgct cgagcacctc gggatcggac
     1321 tcggtgaggc gcgactccac cttcacctca cccggcagct ccacgtaccc gaccgcgaac
     1381 ctggagtagt cctcaccctt cggccccgtg tacggcggcg acggcggctg gaagtcctgg
     1441 gtggtccacg cccacagccg cccgcgccgc gggagcaggt ggtcctccat ctccgtggac
     1501 gcgtcgcgcg gacacgagcc ctgcgcgggg aacgtgacga tcccgcactg cgcgcacttg
     1561 ctgccgatga gctgcggcgc gtcggacggc cacgtgaaca ggccttcgct gatggctcgc
     1621 tcaccgctca tgtgcgtcat gtctccttgt cgccggtcac gcggttcgct cgctgtgcgc
     1681 gtgcccggag ctcatctttc atcaccttgc cggtggcgtt gatcggcagc tcgtcgacga
     1741 actcgacgaa cctcggcacc ttgaagttcg ccatctcgtc gcgcgaccac ccgatcacct
     1801 cgtcggtgtc gagtggcggg ccgttgccgt cgagcaccac gaacgccatg cccacctcgc
     1861 cgagccgcgc gtcgggcacg ccgatcaccg cggcctgcga gatgcgcggg tgacgtaaca
     1921 gcaggttctc gatctcggcg gggtacgcat tgaagccgcc gacgatgaac atgtccttga
     1981 tgcgcccgac gatgcgcacg tatccgtcct cgtcgatggt gccgaggtcg ccggtgtgca
     2041 ggaacccctc gtcgtcgaac gcggccgcgg tggcgtcggg atcgtcgagg tagcc
//
LOCUS       DASUGA010000627         2537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1763358, whole genome shotgun sequence.
ACCESSION   DASUGA010000627 DASUGA010000000
VERSION     DASUGA010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763358"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VFX21_12385"
     CDS             <1..430
                     /locus_tag="VFX21_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007515419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5096812.1"
                     /translation="YAALRAVPIPDEVVARCFHAASLLREHRGDGHIVALMSQDVGRL
                     EAHVLFALGTDVPAEKFGRIHHLPRAQLAAVIDGMRDRGLIGDDGWLSESGRAVRARV
                     ESLTDDLAAQPYECLEPRELDELMAALEPLATLLGAAQEW"
     gene            443..1639
                     /gene="rsmI"
                     /locus_tag="VFX21_12390"
     CDS             443..1639
                     /gene="rsmI"
                     /locus_tag="VFX21_12390"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX5096813.1"
                     /translation="MRFTCHGHPNVTGTHDKTLELTRDLDIGVRATCIVGVGANYDEG
                     ALLKLRGEIEVVLEGNGASESFTATVSPFFLGGDSLVFRRGPGLRTRTFAYDASKTAA
                     DLDRRLMHADSVLVSMRATGVRAVGALFVVSLPIGNDDDLSPRAARVLERADVVLAED
                     TRRVHALAQRLGTRTAPTLSYHDRNEDERVAEVVERIAAGERIALVSDAGTPLLSDPG
                     YVVVRETVARDLPVSPVPGPSAALAVAAVSGLPVHEILFAGFLPRTTSKRQSELQRLF
                     SSGATVVLYEAPNRVAALLGDIDAVAPATRVCIGREVTKDFEEFAYGTPHELAARFAA
                     DARGEFTIVLSPPAPDAPDTTAEDELLRALLADGVPPKTLARALARTPGVSRNAAYER
                     VLGLQE"
     gene            1636..2289
                     /locus_tag="VFX21_12395"
     CDS             1636..2289
                     /locus_tag="VFX21_12395"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5096814.1"
                     /translation="MTLTVVVGRNATTRMDAAAFAELREHYDATFVDVGTGDGRFVAH
                     LAAEWPEALVIGIDTAEDNMAEAANKARRKRGTANALFVRSAIEALPQVLHHVADLVF
                     VQLPWGRLLEGIVLGDAAVVGGIAALCRPGARVEVNLNNEIWLDSTPERFADLPLPTP
                     EYVDRVIAPAFATAGIDLGPARMLGAEQAKALPTTWAKRLGHSREHPKFVHVEGTAR"
     gene            2300..>2537
                     /locus_tag="VFX21_12400"
     CDS             2300..>2537
                     /locus_tag="VFX21_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009682117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5096815.1"
                     /translation="MDKHAQINLASWESRVGIHVKSQAYDVDALVAGTRRLSDVVAFD
                     EPYLGDLTGLDVVHLQCHIGTDTLSLARLGARTVT"
BASE COUNT          366 a          897 c          894 g          380 t
ORIGIN      
        1 gtacgccgca ctgcgcgcgg tcccgatccc cgacgaggtg gtggcgcgtt gcttccatgc
       61 cgcgtccttg ctgcgtgagc accggggcga cgggcacatc gtcgccctga tgagccagga
      121 cgtcggccgc ctcgaggcgc acgtgctctt cgccctcggc acggatgtgc cggcggagaa
      181 gttcgggcgc atccaccacc ttccccgggc gcagctcgcc gccgtgatcg acgggatgcg
      241 cgatcgcggc ctgatcgggg acgacggttg gctgagcgaa tcgggccgcg ccgtcagagc
      301 gcgggtcgag tcgctcaccg acgacctcgc cgcgcagccg tacgagtgcc tcgagccgcg
      361 ggagctcgac gagctgatgg ccgcgctcga accgctcgcc acgctgctcg gcgccgccca
      421 ggagtggtag acgcgggggc ggatgcgatt cacctgccac ggacatccga atgtcaccgg
      481 tacgcacgac aagacgctgg agctgacgcg cgatctcgac atcggcgtgc gcgccacgtg
      541 catcgtcggc gtcggcgcga actacgacga aggcgcgttg ctgaagctgc ggggcgagat
      601 cgaggtcgtg ctcgagggca acggtgcctc cgagtcgttc accgcgacgg tgagcccgtt
      661 ctttctcggt ggcgattcgc tggtcttccg gcgggggccg gggctgcgca cccgcacgtt
      721 cgcgtacgac gcgagcaaga ctgcggccga tctggaccgc cggttgatgc atgccgactc
      781 ggtgctcgtg agcatgcggg ccaccggcgt gcgggcggtc ggtgcgctgt tcgtcgtgtc
      841 gctgcccatc gggaacgacg acgatctctc gccgcgtgcg gcgcgggtgt tggagcgcgc
      901 ggacgtcgtg cttgccgaag acacacgccg cgtgcacgcg ctcgcgcaac ggctcgggac
      961 gcgcaccgcg ccgacgctga gctatcacga ccgcaacgag gacgagcgcg tcgcggaagt
     1021 cgtcgaacgc atcgcggccg gtgagcgcat cgcgctcgtc agcgacgcgg gcacgccgct
     1081 gctcagcgac ccgggctacg tcgtcgtgcg ggagaccgtt gctcgtgatc tgcccgtttc
     1141 accagtgccg ggtccgtcgg ccgcgctcgc agtcgcggcg gtgtcggggc ttccggtgca
     1201 cgagatcctg ttcgccggct tcttgccgcg tacgacgtcc aagcggcagt cggagctgca
     1261 gcgcttgttc tcgagcggcg cgaccgtcgt gctgtacgaa gcgcccaatc gcgtcgcggc
     1321 cctgctcggc gatatcgatg ccgttgcgcc cgcaacgagg gtctgcatcg ggcgcgaggt
     1381 caccaaggac ttcgaagagt tcgcgtacgg cacaccgcac gagttggccg cgcgcttcgc
     1441 ggccgacgcg cgcggcgaat tcacgatcgt gctctctccg cccgcgccgg acgcgcccga
     1501 cacaaccgcg gaggacgagc tcctgcgcgc gctgctcgcc gacggtgtgc cgccgaagac
     1561 cttggcccgg gcgttggcgc gcacgcccgg tgtcagtcgc aacgccgcgt acgagcgcgt
     1621 cctcgggctc caggagtgac ccttaccgtc gtcgtcgggc gcaacgcaac gaccaggatg
     1681 gacgccgccg cgttcgccga gctgcgcgag cactacgacg cgacgttcgt cgacgtcggg
     1741 accggcgacg gccgcttcgt cgctcatctc gccgccgagt ggccggaagc gctggtcatc
     1801 ggcatcgata ccgccgagga caacatggcc gaggcggcca acaaggcgcg ccgcaagcgg
     1861 gggacggcga acgcgttgtt cgtgcgcagc gcaatcgagg cgttgccgca ggtcctgcac
     1921 catgtggccg acctggtgtt cgtccagctg ccgtggggcc ggctgctcga aggcatcgtg
     1981 ctcggcgacg ccgcggtcgt gggcggcatc gccgcgttgt gccggccggg agcgcgcgtc
     2041 gaggtcaacc tcaacaacga gatctggctc gactcgacac ccgaacgctt cgccgacctg
     2101 ccgttgccga cgcccgaata cgtcgaccgt gtgatcgcgc ccgcgttcgc gaccgcgggc
     2161 atcgacctcg gtccggcgcg catgctcggc gccgagcaag ccaaggcatt gccgacgacc
     2221 tgggcgaagc ggctcggcca cagccgcgag cacccgaaat tcgtccacgt cgagggaacc
     2281 gcccgttagc gtccaccgga tggacaagca cgcccagatc aacctcgcga gctgggaatc
     2341 gcgcgtcggc atccacgtga agtcgcaggc ctacgacgtc gacgcgctgg tcgcgggcac
     2401 ccgccgcttg agtgacgtcg tcgcgttcga cgagccgtac ctcggtgacc tcaccggcct
     2461 cgacgtcgtc cacctgcagt gccacatcgg caccgacacc ctctccctgg cccggctcgg
     2521 cgcccggacc gtgaccg
//
LOCUS       DASUGA010000628         2134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1590173, whole genome shotgun sequence.
ACCESSION   DASUGA010000628 DASUGA010000000
VERSION     DASUGA010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590173"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VFX21_12405"
     CDS             <1..517
                     /locus_tag="VFX21_12405"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX5096816.1"
                     /translation="AINFMLRWARGLVCMPCDGARLDELEIGPMLPAGTEGCDTAFTV
                     SIDHRAAGSGIGAADRALTVRKILDADSRAEHFVRPGHVFPLRARPGGVLERRGHTEA
                     AVDLARLAGLPPVAVICEVLHDDGSPARFQYLEMFAQEHRVAMVSVAQIVEHRERAAV
                     GASTRTRTLSL"
     gene            complement(518..1528)
                     /locus_tag="VFX21_12410"
     CDS             complement(518..1528)
                     /locus_tag="VFX21_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096817.1"
                     /translation="MRIRLEPEDEYMHKLEDASNFNESMYFNVYDPAEGIGGFLRLGN
                     RANEGYAELTTCLYLPDGRVAFMFKRPEIDNNDAFDAGGTHFEVITPFEALRTTYDGK
                     VALLDDPLLMADPRKAFTESPYADAHVDLTHTAVSPMYGGEPVNDDGSALSEDYSGGF
                     ARGHYEAHMKVTGTIRVGDEEWAVNGFGLRDHSWGPRFWSVPWYYRWLTANFGDDLGF
                     VVSIVTARDGSERIGGMILRDGAYEHIHDAAISTSWTGGDMYHQSLRATATTKHDTYE
                     IEGKVLNLIPLRNRRTTPDGEKLVTRISEGLTEWKMRKRTGFGLSEYLDQIIDDKPVG
                     AS"
     gene            1587..>2134
                     /locus_tag="VFX21_12415"
     CDS             1587..>2134
                     /locus_tag="VFX21_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX5096818.1"
                     /translation="MTNPAITEVTDGLEFPEGPVCMPDGSVLVAELRGKRVTRVHPDG
                     KKETVAEPGGSPNGLAIGPDGALYVANSGGWQFHDVMGMLIPEGEQPADYSGGRIERI
                     DLDTGDVQVLYTECNGHELVGPNDLVFDIHGGFYFTDHGKTRGRVKTIGAIYYALPDG
                     SSIKEVVFPLDSPNGIGLSPDGS"
BASE COUNT          325 a          710 c          726 g          373 t
ORIGIN      
        1 agcgatcaac ttcatgcttc gctgggcacg gggcctcgta tgcatgccgt gtgacggcgc
       61 ccgcctcgac gagctcgaga tcggcccgat gctcccggcc ggcaccgaag ggtgcgacac
      121 cgcgttcacg gtctcgatcg accaccgtgc cgcggggagc ggcatcggcg cggccgatcg
      181 cgcgctcacg gtgcgcaaga tcctcgacgc cgactcgcgt gccgagcatt tcgtgcgccc
      241 cggccacgtg ttcccgctgc gcgcccgccc cggtggcgtg ctcgaacggc gcggtcacac
      301 cgaagctgcg gtcgacctcg cgcgcctcgc gggcctgccg ccggtcgcgg tcatctgcga
      361 agtgctgcac gacgacggtt cgccggcacg gttccagtat ctcgagatgt tcgcccagga
      421 gcaccgcgtc gcgatggtgt cggtggcgca gatcgtcgag caccgcgagc gcgccgccgt
      481 cggcgccagc acccgcaccc gcaccctgtc tttgtagcta gctcgcgcct actggcttgt
      541 cgtcgatgat ctgatcgagg tactcggaga ggccgaagcc ggtgcgcttg cgcatcttcc
      601 attcggtgag gccctcggag atgcgcgtca ccagcttctc gccgtcgggc gtcgtgcggc
      661 ggttgcgcag cgggatgagg ttgaggacct tgccctcgat ctcgtaggtg tcgtgcttcg
      721 tcgtggccgt ggcgcgcagg ctctggtggt acatgtcgcc gccggtccac gaggtcgaga
      781 tcgcggcgtc gtggatgtgc tcgtaggcac cgtcgcgcag gatcatgccg ccgatgcgct
      841 cgctgccgtc gcgtgcggtg acgatcgaga cgacgaaacc gaggtcgtcg ccgaagttgg
      901 ccgtcagcca gcggtagtac cacggcaccg accagaagcg cgggccccac gagtggtcgc
      961 gcaggccgaa gccgttcacg gcccattctt cgtcgccgac gcggatggtg cccgtgacct
     1021 tcatgtgcgc ctcgtagtga ccacgcgcga agccgccgga gtagtcctcc gacagcgcgg
     1081 aaccgtcgtc gttcaccggt tcgccgccgt acatcggcga caccgccgtg tgcgtgaggt
     1141 cgacgtgcgc gtcggcgtac gggctctcgg tgaacgcttt gcgcgggtcg gccatgagca
     1201 gcgggtcgtc gagcagcgcg accttgccgt cgtacgtcgt tcgcagcgct tcgaacggtg
     1261 tgatcacttc gaagtgcgtc ccaccggcgt cgaacgcgtc gttgttgtcg atctcgggcc
     1321 gcttgaacat gaacgcgacg cgcccgtcgg ggaggtacaa gcaggtcgtg agctccgcgt
     1381 agccctcgtt cgcgcggttg cccaggcgca ggaacccgcc gatgccttcg gccgggtcgt
     1441 agacgttgaa gtacatcgac tcgttgaagt tcgacgcgtc ctccaacttg tgcatgtatt
     1501 cgtcttcggg ctccaagcgg attctcatgg cgccgatcag agtcggttct cgaccgaccg
     1561 gtcaagacgg tgcaaggatg ccgaacatga cgaatccggc gatcaccgaa gtcaccgacg
     1621 gcctcgagtt ccccgaaggt ccggtctgca tgcccgacgg cagcgtcctg gtggccgaac
     1681 tgcgcggcaa gcgtgtcacg cgggtgcacc cggacgggaa gaaggagacc gtcgcggaac
     1741 cgggcggcag tccgaacggt ttggcgatcg gcccggacgg cgcgttgtac gtcgccaaca
     1801 gcggcggctg gcagttccac gacgtgatgg gaatgttgat tcccgagggt gagcaacccg
     1861 cggactactc cggcgggcgc atcgagcgca tcgatctgga caccggcgac gtgcaggtgc
     1921 tctacaccga gtgcaacggc cacgagctcg tcgggccgaa cgacctcgtg ttcgacatcc
     1981 acggcggctt ctacttcacc gaccacggca agacgcgcgg ccgcgtcaag acgatcggcg
     2041 cgatctacta cgcactcccc gacggttcgt cgatcaagga ggtcgtgttc ccgctcgact
     2101 cgccgaacgg tatcgggctg tcgcccgatg gttc
//
LOCUS       DASUGA010000629         2251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_827830, whole genome shotgun sequence.
ACCESSION   DASUGA010000629 DASUGA010000000
VERSION     DASUGA010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827830"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1596)
                     /locus_tag="VFX21_12420"
     CDS             complement(37..1596)
                     /locus_tag="VFX21_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006344740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5096819.1"
                     /translation="MVSFNLADLFELVADAVPDRVAVVGGDVRRTYGALDERATRLAH
                     AIDVEPGRHVGLCLRNSIAHVELMLACYKRRVVPVNVNWRYTDDELDYLARDAQLVAL
                     WRDEDTGTDAYEDLVASGSPARDFGERSADDHYVLYTGGTTGMPKGVVWRQEDIFFAA
                     LGGGNPGGPPITAPGEIVASVLDNPSQRLRAFLRPDEPGPPQFVSLALGPLMHASGQW
                     SALGTLLGGGKVVLVTDTSIDMTRVLDLVEREAVNACNLVGDASARPMLAALAAEPQR
                     WDTSSLRLLGSGGSILSGAVKDELMRALPSVLAIVEGVGSSEAPAQAVAVTRRDGGVN
                     QSLTFAPKAETIVVDDDLRPIPAGSGRAGRLATRGRVPLHYYNDPERSARTFVEIDGR
                     RWSLPGDMATIDADGTIHLLGRGSLCINTGGEKVYPEEVEAVLKTAPGVADAAVLGVA
                     DDVYGERVVALVAPADRATPPTLDDVQQYARTRLAGYKLPRALHVVDDIVRTPAGKID
                     YAWARSVATVS"
     gene            1614..>2251
                     /locus_tag="VFX21_12425"
     CDS             1614..>2251
                     /locus_tag="VFX21_12425"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX5096820.1"
                     /translation="MTLEDVLMRDDGFLPKARYTSPDFAALEFERLWSRVWQVACRVE
                     QVARPGDYVEYTIGDESVLVVGGDDGTIRAFHNTCLHRGSQLATDVGTFADGCIRCPY
                     HAWKYALDGELVEIVDRDEFTNIPDGMTMHLRSVRVDTFGGFVFVNLDADAEPLADFL
                     DPLASVLAPYHLERMRFRSYLTTIIDANWKAVVDAFNEGYHVQGLHSQILRWT"
BASE COUNT          338 a          773 c          778 g          362 t
ORIGIN      
        1 catgccacgt cgatgtgagc gcgtccctcg cggtcgtcat gacacggtcg cgacggaccg
       61 tgcccacgcg tagtcgatct tgccggcggg cgtgcggacg atgtcgtcga cgacgtgcaa
      121 cgcacgtggc agcttgtaac ccgcgagtcg cgtgcgcgcg tactgctgga cgtcgtcaag
      181 cgtcggcggt gtcgcgcggt cggccggtgc cacgagcgcg acaacgcgtt cgccgtagac
      241 gtcgtcggcg acgccgagca cggctgcgtc ggcgacgccg ggcgccgtct tcaggaccgc
      301 ctcgacttcc tcggggtaca ccttctcgcc gccggtgttg atgcacaacg acccgcggcc
      361 gagcagatga atcgtgccgt cggcgtcgat cgtcgccatg tcgcccggca acgaccagcg
      421 ccggccgtcg atctcgacga acgtgcgggc gctgcgttcg gggtcgttgt agtagtgcag
      481 cggcacgcgc ccacgggttg cgagccggcc cgcccgcccg ctccccgcgg gaatcgggcg
      541 gaggtcgtcg tcgacgacga tcgtctctgc cttcggtgcg aacgtcagcg actggttcac
      601 gccaccgtcg cgccgcgtga ccgcgaccgc ttgcgcgggc gcctcggacg atccgacacc
      661 ttcgacgatc gcgagcaccg aagggagtgc gcgcatcagc tcgtccttca cggcacccga
      721 caggatgctg ccgccggaac cgagcaagcg cagtgacgac gtatcccacc gttgcggttc
      781 ggcggcgagc gcggcgagca tcgggcgcgc gctcgcgtcg ccgacgaggt tgcacgcgtt
      841 caccgcttcg cgttcgacga ggtcgagcac acgggtcatg tcgatgctgg tgtcggtgac
      901 gagcacgacc ttgccgccgc cgagcagcgt gccgagcgcg gaccactgcc cgctcgcatg
      961 catcagcgga ccgagcgcca atgagacgaa ctgcggcgga ccgggctcgt ccggcctcag
     1021 gaaggcgcgg agccgttggg agggattgtc gaggacggag gcgacgattt ccccgggggc
     1081 ggtgatcggc ggtccacccg ggttcccgcc gccgagcgcg gcgaagaaga tgtcttcttg
     1141 gcgccacacc acgcccttcg gcatcccggt cgtgccgccg gtgtagagca cgtagtgatc
     1201 gtcggccgac cgctcgccga agtcgcgcgc cgggcttccg ctcgcgacca agtcctcgta
     1261 ggcgtcggtg ccggtgtctt cgtcacgcca cagggccacg agctgcgcgt cgcgggcgag
     1321 gtagtcgagc tcgtcgtcgg tgtagcgcca gttcacgttg accggcacga cgcgccgctt
     1381 gtagcacgcc agcatcagct cgacgtgcgc gatcgagttg cgcaggcaca gtccgacgtg
     1441 gcggccgggc tcgacatcga tcgcgtgggc gagccgcgtc gcccgttcgt cgagtgcgcc
     1501 gtaggttcgg cggacgtcgc cgccgacgac ggcaacccgg tcgggcaccg cgtcggcgac
     1561 cagttcgaac agatccgcaa ggttgaacga gaccactagt gtccgattct ggcatgacgc
     1621 tcgaagatgt gttgatgcgc gacgacggct tcctgcccaa ggcgcgctac acgtcacccg
     1681 acttcgccgc cctcgagttc gagcggctgt ggagccgggt gtggcaggtc gcgtgccgcg
     1741 tggagcaagt cgcccggccc ggtgactacg tcgagtacac gatcggcgac gagtcggtgc
     1801 tcgtggtcgg cggcgacgac ggcacgatcc gcgcctttca caacacgtgc ttgcatcgcg
     1861 gctcccagct cgcgaccgac gtcgggacct ttgccgacgg gtgcatccgc tgcccgtacc
     1921 acgcgtggaa gtacgcgctc gacggcgaac tggtggagat cgtcgaccgc gacgagttca
     1981 ccaacatccc cgacggcatg accatgcatc tccgctcggt gcgcgtcgac accttcggcg
     2041 gcttcgtgtt cgtgaacctc gacgcggatg ccgaaccgct ggccgacttc ctcgacccgt
     2101 tggcgagtgt gctggcgccg taccacctcg aacgcatgcg cttccgcagc tacctcacga
     2161 cgatcatcga cgcgaactgg aaggccgtcg tcgacgcgtt caacgagggc tatcacgtgc
     2221 agggtctgca ctcgcagatc ctccggtgga c
//
LOCUS       DASUGA010000630         7147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_60450, whole genome shotgun sequence.
ACCESSION   DASUGA010000630 DASUGA010000000
VERSION     DASUGA010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60450"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="VFX21_12430"
     CDS             <1..1255
                     /locus_tag="VFX21_12430"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5096821.1"
                     /translation="VLEARDRVGGRVWTKDVDGIPIDIGGTWLGPGQDFVYDLVKELG
                     LSTYATHTAGETVFVHGHDVQRYKGQVPKLNPITVASLGLGMARLDRMAKKVPLDEPW
                     VGSRAPRWDGRSMADWIRANVPEKTSAALLGAIVRGLMTCDPSEVSLLHMLYLVQSAR
                     GLNRLLSIEGGYQQDMVKGGAQSMANIMADELGPSVRLSSPVRAIHHDDRSVVVESDG
                     VTVRAGHAIVATPPALAHKIDFTPGLPIDRAQVLQRMPAGCVLKTVVVYDRAFWRDEG
                     LRGETVALGSSIESTLDASPPAGTPGELCALAFGPMARRLTELGADARRDHVLGELTV
                     RFGPKARHPRHYVEVDWAQEQWSEGCYLAHLPPGVLTQYGHVLRAPAGRIHWAGTETA
                     TVSHGSIDGAIRSGVRAAQEILATN"
     gene            1262..2758
                     /locus_tag="VFX21_12435"
     CDS             1262..2758
                     /locus_tag="VFX21_12435"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5096822.1"
                     /translation="MGITARVSLARARDVTLGTFLERFAHARANRVLVEQPDGLRLTY
                     AEAADLVARAAGTVRTKITTGDRVVVALPNGYPLLLACLAVCRAGGVAVPVNERMAAP
                     EIDYIEQDANAVWRIDDFDVLVDSPPTDAVPVSAEAVAMLLYTSGTTGRPKGAQLTHR
                     SLLSGMRAGALAPEALLAHGCVTGMPVAHIAGLQMLLGLAAAGIPVFLLTHFRPTDAL
                     DAIEQRRPMLFLGVPAMYRMMLEAGAADRDLSSVRMWSSGADTLPPDIVDAFQRMGRG
                     IPVPFTRLGIGRASFVDGYGMVELAGGVAVRVFGPIPIPGVRGLLRPMQGHEVRIVDD
                     DGHDVGRGEVGELLVRGPGVMRGYYGRDDATAATVSGDGWLRTGDLARPRHLGFFELA
                     GRKKDVIKHGGYSVFAVEVERALEEHPAVAEAAVLPLPDTAKGEVPVAAVRLHPDAVA
                     TPETLRAHCAAILSDYKVPRQIVVVDELPRTGTEKVQKRDLVPLFDAQ"
     gene            complement(2763..3398)
                     /locus_tag="VFX21_12440"
     CDS             complement(2763..3398)
                     /locus_tag="VFX21_12440"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5096823.1"
                     /translation="MLEQPPAIAVPFFLSTPQAPPPWPGVVVVMEGGGITPQLLRVCE
                     RLAGEGYATIAPDLYWRFGGSDPDRLVDYVPALRHADGRADIAQCAAFLRAIGATKVG
                     ITGFCMGGGYAYLAARSDVAVDASVAFYGGGVAQHLGDAHCPLLLFFGGRDEWISADD
                     VSAVERQHPGNVVVYADAGHGFLRDGSDDYNEPAATDAWDRLLAFFGEHLR"
     gene            complement(3414..4406)
                     /locus_tag="VFX21_12445"
     CDS             complement(3414..4406)
                     /locus_tag="VFX21_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5096824.1"
                     /translation="MQHLRLGRTGLQVSRLCLGTMTFGLQCDEETSIAIMNRAADGGM
                     TFFDTADVYPLGGDLTTVGRTEEIVGRWLTGQRDQYVIATKCFGPTGSRPWDAGNSRR
                     HIMDAIDASLRRLQTDYVDLYQLHFDDPNTPLDETLGALDDLVRAGKVRYVGCSNFLA
                     FRLARAIGRSEALGVTRFDSVQPRYNLIFREFERELLPLCLQEGIGVIPYNPIAGGML
                     SGKHDRSKAPSDGSRFTLGTAGKMYQDRYWHDNVFDTVDELVKIAESIAMPMPTMALA
                     WVLEHPAITSPIVGASRPDQLDATLAAVDVHLERDLVERLNDLTAPYRRGDAPR"
     gene            complement(4414..5172)
                     /locus_tag="VFX21_12450"
     CDS             complement(4414..5172)
                     /locus_tag="VFX21_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096825.1"
                     /translation="MHRTRNLLIDIAVVVILVSHAVAFVVIRQHRTRYDGEARAIASW
                     MHDQGEDRVAWPAELRGSVPALLSANVTPVLVDDARVAPQQLAVNDTGVVVTRDGDRL
                     GDVTGESFDAGDLTAHVVKLDAAAATPLASGDIVGQNIARPYRFAPIRNSLRLTEDEP
                     FRMELTLDPGRYQLAVEAFAPQRQRDLVTTIATKPVQRTTTRLRQVVQAPIVVPFTVG
                     PDDAQPVKVRLAVAGDGKPQAFVHQWTVRRTDGA"
     gene            5310..6038
                     /locus_tag="VFX21_12455"
     CDS             5310..6038
                     /locus_tag="VFX21_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017718178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX5096826.1"
                     /translation="MTAVEDERLHRKQRLAAAFRWFSRYGFDEGVAGHITARDPELLD
                     HFWVNPFAMHFGMIKVSDLILVNDQGKVVEGNAAVNSAAFAIHSQIHAARPDVIAAAH
                     SHSMHGKAWSTLGRLLDPITQDSCAFYRDHALFDDYTGVVLDTEEGKRIGVALGEHKA
                     VILRNHGLLTVGHSVEEAAFWFIVMDRTCQAQLLSEAAGTPVLIDPEMAALTAKQVGG
                     HAAGLMGFKPLFEKIVAEEPDLLD"
     gene            complement(6053..>7147)
                     /locus_tag="VFX21_12460"
     CDS             complement(6053..>7147)
                     /locus_tag="VFX21_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096827.1"
                     /translation="VLYFNGAVGLLISVLHANVWEVDAQHPLGNQLVAPAGAVAPGGG
                     TDYTAPNYRRAAVVGEQLALASERLIDNAKLVHNTKTSYQVEPFVTRLSNFGFRVLLV
                     IDPDTGRSQLGHEAGPLLVCPDAPANYDTCVSDNLESETDDDIGVDARVGDFLQSAVE
                     YVHIGNVGMMFLPGEISSELTIGLPRDFRTAPEHWYAEPPGTHAFGADYQTPGYVTRR
                     MHDKYEWTIGLGSDELGYIIPISNFRVSCVGDVFVGPGACAFLHAYGAIEYPDAIAGS
                     TCKRTTDDPATLDEYADVVADVISASCRYGQAFGEANGHYEETNSASWDLAQATLDAV
                     ARITGDDDATQVNNKFPGWWQGYLPPGSLP"
BASE COUNT         1082 a         2374 c         2523 g         1168 t
ORIGIN      
        1 ggtgctcgaa gcgcgcgatc gcgtcggtgg gcgggtgtgg acgaaggatg tcgacgggat
       61 cccgatcgac atcggtggca catggctcgg accgggccag gacttcgtgt acgacctcgt
      121 gaaggagctc gggctcagca cgtacgcgac gcacacggcc ggcgagaccg tcttcgtgca
      181 cggccacgac gtccagcgtt acaaaggtca ggtcccgaag ctgaacccga tcacggtcgc
      241 gagcctcggg ctcgggatgg cgcgcctgga ccgcatggcc aagaaggtcc cgctcgacga
      301 accgtgggtc ggatcacgcg cgccgcggtg ggacggccgc tcgatggcgg actggatccg
      361 cgccaacgtg cccgagaaga cgagcgcggc gctgctcggc gcgatcgtgc gcggattgat
      421 gacgtgcgac cccagcgaag tgtcgctcct gcacatgctc tacctcgtgc agtcggcgcg
      481 cggcctcaac cgcttgctgt cgatcgaagg cggctatcaa caagacatgg tcaagggcgg
      541 cgcgcagtcg atggcgaaca tcatggccga cgagctcggc ccgtcggtgc gcctctcgtc
      601 gccggtgcgg gccatccacc acgacgatcg cagcgtcgtc gtcgaaagcg acggtgtgac
      661 ggtgcgcgcc gggcacgcga tcgtggcgac gccgcccgcg ctggcccaca agatcgactt
      721 cacacccggc ttgccgatcg atcgcgcgca ggtgctgcag cgaatgccgg cgggttgcgt
      781 gctcaagacc gtcgtcgtgt acgaccgcgc cttctggcgg gacgaggggc tgcggggcga
      841 aaccgtcgcg ctgggatcgt cgatcgagtc gacgctcgac gcgtcgccgc cggcgggaac
      901 gccgggtgag ctgtgcgcgc tcgcgttcgg cccgatggcc cgccggctga cggagctcgg
      961 cgccgacgcg cggcgcgacc acgtgctcgg tgagttgacc gtgcgcttcg gcccgaaggc
     1021 acgccaccct cgccactacg tcgaagtcga ctgggcgcag gaacagtgga gcgaagggtg
     1081 ctatctcgcg cacctcccgc cgggcgtgtt gacccagtac gggcacgtct tgcgggcacc
     1141 cgccggacgc atccactggg ccggcaccga gaccgcgacc gtctcgcacg gctccatcga
     1201 cggcgccatc agatccggtg tgcgcgccgc acaggagatc ctcgcgacaa actgatcacg
     1261 tatgggcatc acggctcggg tctcgctcgc acgggcgcgc gacgtcacgc tcggcacgtt
     1321 cctcgaacgg ttcgcgcacg cgcgcgcgaa tcgcgtgctc gtcgagcagc ccgacggtct
     1381 gcggctgacg tacgccgaag cggcggatct cgtcgcgcgc gcggccggga cggtgcgcac
     1441 gaagatcaca accggcgacc gcgtcgtggt tgcgctgccc aacggctatc cgcttctgct
     1501 cgcgtgcctg gccgtctgcc gcgccggtgg tgtcgcggtg cccgtcaacg aacgcatggc
     1561 cgcgcccgag atcgactaca tcgagcagga cgcgaacgcg gtctggcgca tcgatgactt
     1621 cgacgtgttg gtcgactcgc cacccaccga tgcagtgccg gtctccgccg aggcggtcgc
     1681 catgttgctc tacacctcgg gcacgaccgg ccggccgaag ggtgcgcagc tgacccaccg
     1741 atcgctgctg agcggcatgc gagccggcgc gctggccccc gaagccctcc tcgcgcacgg
     1801 atgcgtcacc gggatgccgg ttgcgcacat cgccgggctg cagatgctgc tgggcctggc
     1861 cgccgccggc attcccgtgt tcctgctcac gcacttccgc ccaaccgacg cgctcgacgc
     1921 gatcgaacag cggcggccga tgctgttcct cggtgtgccc gcgatgtacc gcatgatgct
     1981 cgaagcgggg gcggctgatc gcgacctgtc atcggtacgg atgtggtcgt cgggtgccga
     2041 cacgctgccg cccgacatcg tcgacgcgtt ccagcggatg gggcgcggca tccccgtgcc
     2101 cttcacgcgt ctcggcatcg ggcgcgcctc gttcgtcgac ggctacggca tggtcgagct
     2161 cgcgggcggc gtcgcggtcc gcgtgttcgg accgatcccg atcccgggcg tccgcgggtt
     2221 gttgcggccg atgcaaggtc atgaagtgcg catcgtcgac gacgacggcc acgacgtcgg
     2281 gcgcggcgaa gtcggcgagt tgctcgtacg cgggccgggc gtgatgcgcg gctactacgg
     2341 ccgcgacgac gccaccgcgg caacggtgtc cggcgacggt tggctgcgta ccggtgatct
     2401 cgcgcggccg cgccatcttg gtttcttcga gctcgcgggc cgcaagaagg acgtcatcaa
     2461 gcacggcgga tactcggtgt tcgcggtcga ggtcgagcgc gcgctggagg agcacccggc
     2521 agtcgcggag gccgcggtgc tcccgctgcc ggatacggcg aagggtgagg tgcccgtcgc
     2581 cgcggtgcgc ctccatccgg acgcggtggc cacgcccgag acactgcggg cgcactgcgc
     2641 ggcgattctc agcgactaca aggtgccacg ccagatcgtc gtggtcgacg agctgccgcg
     2701 caccggcacc gagaaggtgc aaaagcgcga cctcgtgccg ctgttcgacg cgcagtagcg
     2761 cgtcaacgga ggtgctcgcc gaagaacgcg agcagccggt cccatgcgtc ggtggcggcg
     2821 ggctcgttgt agtcgtcgga gccgtcgcgc aagaacccgt gacctgcgtc tgcatacacg
     2881 accacgttgc ccgggtgttg tcgttcgacc gcagagacgt cgtccgcgct gatccactcg
     2941 tcgcgtccac cgaagaacag caacagcggg cagtgcgcgt cgccgaggtg ctgtgcgacg
     3001 ccaccgccgt agaaggccac cgacgcatcg accgcgacat cggagcgcgc cgcgaggtac
     3061 gcgtagccgc cgcccatgca gaagccggtg atgccgacct tggtcgcacc gatcgcgcgc
     3121 aagaacgccg cgcactgcgc gatgtcggcg cgtccgtcgg cgtgacgcag tgcggggacg
     3181 tagtcgacca ggcgatcggg atcgctgccg ccgaagcgcc agtacaggtc cggcgcgatc
     3241 gtggcgtagc cctcgcccgc cagccgttcg cagacccgca acaactgcgg cgtgatgccg
     3301 ccgccctcca tcacgacgac gacaccgggc cacggtggcg gcgcctgggg tgtcgacagg
     3361 aagaacggga cggcgatggc aggcggttgc tcgagcacgg gcgggagtat ggctcagcgg
     3421 ggtgcgtcac cccgtcgata cggtgccgtg aggtcgttca accgctcgac gagatcgcgc
     3481 tcgaggtgca cgtcgacggc cgcgagcgtc gcgtcgagtt ggtcggggcg gctggcgccg
     3541 acgatcggtg acgtgatcgc cggatgctcc agcacccacg cgagcgccat tgtcggcatc
     3601 ggcatggcga tcgactcggc aatcttcacg agctcgtcga ccgtgtcgaa cacgttgtcg
     3661 tgccagtacc ggtcttggta catcttgccc gcggtgccga gcgtgaagcg gctgccgtcc
     3721 gacggcgcct tcgaccggtc gtgcttgccg gagagcatcc cgcccgcgat cgggttgtag
     3781 ggaatcacgc cgatgccttc ttgcaggcac agcggcagca gctcgcgttc gaactcgcgg
     3841 aagatgaggt tgtagcgcgg ttgcaccgag tcgaaccggg tgacgccgag cgcttcactg
     3901 cggccgatcg cgcgcgccag gcggaacgcg aggaagttcg agcacccgac gtaccggacc
     3961 ttgccggcgc gcacgagatc gtcgagtgcg ccgagcgttt cgtcgagcgg cgtgttgggg
     4021 tcgtcgaagt gcagttggta gaggtcgacg tagtccgttt gcaggcgacg cagcgacgcg
     4081 tcgatcgcgt ccatgatgtg ccgccggctg ttgccggcgt cccatggccg tgagcccgtc
     4141 gggccgaagc acttcgtcgc gatgacgtac tggtcacgtt gaccggtcag ccaccgaccg
     4201 acgatctcct ccgtgcgccc gacggtcgtg agatcaccgc cgagcggata gacgtcggcc
     4261 gtgtcgaaga acgtcatgcc tccatcggcg gcacggttca tgatcgcgat cgaggtttcc
     4321 tcgtcgcact ggagcccgaa ggtcatcgtg ccgaggcaca acctcgacac ttggaggccg
     4381 gtccggccga gacgtaggtg ttgcacgggt gcatcatgcc ccgtctgtcc gtcgcactgt
     4441 ccactggtgc acgaatgctt gcggtttgcc gtcgccggcg acggcgagac gcaccttgac
     4501 gggctgcgcg tcgtccggcc cgacggtgaa cggcaccacg atcggcgctt gcacgacctg
     4561 gcgcaggcgc gtcgtcgtgc gttgcacggg cttcgtcgcg atcgtggtca cgaggtcgcg
     4621 ctggcgctgg ggtgcaaacg cctcgaccgc gagttggtag cggccggggt cgagtgtgag
     4681 ctccatccgg aacggctcgt cctcggtcag gcggaggctg ttgcggatcg gcgcgaagcg
     4741 gtaggggcgc gcgatgttct ggccgacgat gtcaccggat gcgagcggtg tcgcagccgc
     4801 ggcgtcgagc ttcacgacat gcgcagtgag gtcaccggcg tcgaagctct cgccggtgac
     4861 gtccccgagc cgatcgccgt cgcgggtgac gacgacgccg gtgtcgttga cggcgagctg
     4921 ctgcggcgcg acgcgcgcgt cgtcgaccag caccggcgtg acgttcgccg acaacagtgc
     4981 gggcacgctg ccgcggagct cggccggcca tgcgacccgg tcctcaccct ggtcgtgcat
     5041 ccaggatgcg atcgcgcgcg cttcgccgtc gtagcgcgtg cggtgctggc ggatcacgac
     5101 gaacgccacc gcgtggctga ccaggatgac gacgacggcg atgtcgatca gcaggttgcg
     5161 cgtccggtgc actgctcgcc ttggtccgtg gatgccgcga ccgccagcgt tccacacccg
     5221 gtgtgttcgg tcctcgcggt gcgcaattcg ggcgttcgcg gtgccgaagt atcctgagcg
     5281 gagcccagcg gcccgcgaga ggagatgccg tgaccgccgt cgaggacgaa cgacttcacc
     5341 gcaagcaacg gctcgccgcc gcgttccgct ggttctcgcg ctacgggttc gacgaaggcg
     5401 tcgccggtca catcactgcc cgcgaccccg agttgctcga ccacttctgg gtcaacccgt
     5461 tcgcgatgca tttcgggatg atcaaggtct ccgatctgat cctcgtcaac gaccagggca
     5521 aggtcgtcga aggcaacgcg gcggtcaaca gtgcggcatt cgcgatccac tcgcagattc
     5581 acgccgcgcg ccccgacgtg atcgcggccg cgcacagtca ctcgatgcat ggcaaggcat
     5641 ggtcgacgct cggccggctg ctcgacccga tcacgcagga ctcgtgcgcg ttctaccgcg
     5701 accacgcgct gttcgacgac tacacgggcg ttgtgctcga caccgaagaa ggcaagcgca
     5761 tcggtgtcgc gctcggtgag cacaaggcgg tcatccttcg caaccacggc ttgctcactg
     5821 tcggtcactc cgtcgaggaa gctgccttct ggttcatcgt catggaccgc acgtgtcagg
     5881 cgcagttgct ctcggaagcc gcgggcacgc ccgtgttgat cgatcccgag atggccgcgc
     5941 tcactgcgaa gcaagtcggt ggtcacgcgg cgggtctgat gggcttcaag ccgttgttcg
     6001 agaagatcgt cgcggaggaa ccggaccttc tcgactgacg cactcgtgtg cgctacggaa
     6061 gtgatcccgg tggcaggtag ccctgccacc aacccgggaa cttgttgttc acctgcgtcg
     6121 catcgtcgtc gccggtgatg cgcgcaaccg cgtcgagggt tgcttgcgcg aggtcccagc
     6181 tcgcggagtt cgtctcctcg tagtggccgt tggcttcccc gaacgcctgc ccgtagcggc
     6241 agctggccga gatgacgtcg gcgacgacgt ctgcgtactc gtcgagtgtc gccggatcgt
     6301 cggtggtgcg cttgcacgtc gatcccgcga tcgcgtcggg gtactcgatc gcgccgtacg
     6361 cgtgcaggaa cgcgcacgcg ccggggccga cgaacacgtc gccgacacag ctcacccgga
     6421 agttcgagat cgggatgatg tagccgagct cgtcgctgcc caacccgatc gtccactcgt
     6481 acttgtcgtg catccggcgc gtcacatagc ccggcgtctg gtagtcggcg ccgaacgcgt
     6541 gggtgcccgg cggttccgcg taccaatgct cgggtgccgt gcggaaatcg cgcggcaggc
     6601 cgatggtcag ctcgctggag atctcgccgg gcaggaacat catcccgacg ttgccgatgt
     6661 gcacgtactc gacggcactt tggaggaagt cgccgacgcg cgcgtcgacg cctatgtcgt
     6721 cgtcggtttc gctctcgagg ttgtcggaca cgcaggtgtc gtagttcgcg ggcgcgtcgg
     6781 ggcagacgag cagcgggccg gcttcgtgtc cgagttgcga tcgtccggtg tccggatcga
     6841 tgacgagcaa cacccggaag ccgaagttcg acaagcgcgt cacgaacggc tcgacctgat
     6901 aggaggtctt cgtgttgtgc acgagcttgg cgttgtcgat gagtctctcg ctcgcgagtg
     6961 cgagctgttc gccgacgacc gcggcgcgcc ggtagttcgg cgcggtgtag tcggtgccgc
     7021 cgccgggtgc gacggcgccc gcaggtgcga cgagctggtt gccgagcgga tgctgcgcgt
     7081 cgacttccca cacgttggcg tgcagcacgc tgatcagcag cccgactgcg ccgttgaagt
     7141 agaggac
//
LOCUS       DASUGA010000631         4270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_200727, whole genome shotgun sequence.
ACCESSION   DASUGA010000631 DASUGA010000000
VERSION     DASUGA010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200727"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VFX21_12465"
     CDS             <1..460
                     /locus_tag="VFX21_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5096828.1"
                     /translation="DGATFPFGAHIAVVEVDTETGRVELVRHVAVDDCGRVLNPLIVA
                     GQQHGGVAQGAAQALWEQMVYDDDGNPLTANLMDYAMPSAAELPSYETYNTVTPTPLN
                     PLGAKGIGESATLGSTPAVHNAIVDALSHLGVKHIDMPCTAERVWRALQG"
     gene            457..891
                     /locus_tag="VFX21_12470"
     CDS             457..891
                     /locus_tag="VFX21_12470"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEX5096829.1"
                     /translation="MTFAQPTLRLTGEQYETIIGHCYDGLPNEACGLLLGPMRDGAVT
                     GVISEAWPCRNEAESAVIYTVDGRDYLKASRAAEARGEEIVGVWHSHTHTDPYPSPTD
                     VRQAVDPAWLYAIVSLRDNAPMLRAYRIVDGEIAEVQVVIDR"
     gene            954..1229
                     /locus_tag="VFX21_12475"
     CDS             954..1229
                     /locus_tag="VFX21_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HEX5096830.1"
                     /translation="MAVEIKVPNVLRAQAEGKASLSVEGATVGEVFNNLVTQYPGLRA
                     NLLGDDGGLHKFVNVYKDDDDIRYLDGLDTKVADGDVLSILPAVAGG"
     gene            1226..2164
                     /locus_tag="VFX21_12480"
     CDS             1226..2164
                     /locus_tag="VFX21_12480"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018161125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEX5096831.1"
                     /translation="MRHENVLGLIGNTPLVGVHQLSPNPGVKIWAKLEGQNPGGSSKD
                     RIALKMVELAERDGTLQPGATILEPSSGNTGIGLALVAKLRGYTLRVVMPENVSIERR
                     QLLEIFGAEITLSPGEEGSNGAIRMAEKIHADEPERYTFLFQYGNPANPLAHYEATGP
                     EIWRDCPEVDVFCAGLGTSGTLMGVGKFLKEQNPNVKVVAVEPPSGELVQGLRNLDDG
                     FVPPIFDPAVLDRKLIVRPKDSIEWTRRLLEDCGVFAGISSGAAIAGAVKAAAQLERG
                     TVVTLLPDGGWKYLSSGAWTDPIDEVVERATRINYW"
     gene            2161..3066
                     /locus_tag="VFX21_12485"
     CDS             2161..3066
                     /locus_tag="VFX21_12485"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX5096832.1"
                     /translation="MIDDAVRVLRRGGLVAFPTETVYGLGADAANADALRRLYAVKGR
                     PLDHPVIVHVARAAHLDELARDVPDLAHDLARAFWPGPLTLVVHKRAGRVAPEATGGR
                     ETVGIRVPDHPVALALLDAFDGGVAAPSANRFGKVSPTTAAHVQADLGADVDLVLDGG
                     PARVGVESTIVDVTGAEPVILRLGGVTEAQLAEIAGRALARRTGGEVAAPGTLPSHYA
                     PNARVVVAAPDAVAEIVRDLERSGLRVVALDAPHDAHEYAHVLYARLRNADEPGVDVI
                     VAPEPLDTGGVGAAVMDRLRRASSR"
     gene            3076..3804
                     /locus_tag="VFX21_12490"
     CDS             3076..3804
                     /locus_tag="VFX21_12490"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5096833.1"
                     /translation="MELTVLGCSGSYGAPAGGACSGYAVRAGDAIIWMDCGNGTFANL
                     QQHVNPADLSAVVITHAHPDHCVDIFGLHVLYKYGIDRNMLPVYAPEGVEKVLEHLVG
                     DWTDTFDWNLVADGDTTMIGDAQLRFSRTDHPPPTVAVEIAHEGKRLVYTADTGPEWS
                     VEAFGPGADLVLSEATYAHDDIRAPIHLSAHQAGEAARAARAKRLMLTHIWPTLDPLA
                     SAAEASETFGDTVTLAAPHAVTHI"
     gene            3815..>4270
                     /gene="rph"
                     /locus_tag="VFX21_12495"
     CDS             3815..>4270
                     /gene="rph"
                     /locus_tag="VFX21_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX5096834.1"
                     /translation="MTERVSGRTPDELRSVVFTRDYTEMANGSVLVEFGRTRVLCTAS
                     VEERIPPWLKGSGKGWVTAEYSMLPGSSPERVGREAAKGKQSGRTQEIQRLIGRSLRA
                     VTDLRLMPDTQITVDCDVLQADGGTRTASICGGWIALHDACSRLVGAGLL"
BASE COUNT          719 a         1431 c         1448 g          672 t
ORIGIN      
        1 agacggcgcg acgtttccgt tcggcgcgca catcgcggtg gtggaggtcg acaccgagac
       61 aggacgagtc gagctcgtgc gccacgtcgc cgtcgacgac tgcggtcgag tgctcaaccc
      121 gctcatcgtc gcgggacagc agcacggcgg cgtcgcgcag ggtgcggcgc aggcgttgtg
      181 ggagcagatg gtctacgacg acgacggcaa cccgctcacc gcgaacctca tggactacgc
      241 gatgccgtcc gcggcggagc ttccgagcta cgagacgtac aacaccgtca cgccgacgcc
      301 gctgaacccg ctcggcgcga agggcatcgg cgagtcggcg acgctcggat ccacgcctgc
      361 ggttcacaac gcgatcgtcg acgcgctgtc gcatctcggc gtgaagcaca tcgacatgcc
      421 gtgcacggcc gagcgggtgt ggcgcgcgct gcaaggatga cgttcgcgca gccgacgctc
      481 cgcctgacgg gggagcagta cgaaacgatc atcggacact gctacgacgg cctgccgaac
      541 gaggcgtgcg gactgctgct cggcccgatg cgcgacggcg cggtgaccgg tgtcatcagc
      601 gaggcgtggc catgccggaa tgaagccgaa tcggcggtga tctacaccgt cgacgggcgc
      661 gactacctca aggcgtcgcg cgcggcggaa gcgcgcggcg aagaaatcgt cggcgtctgg
      721 cactcgcaca cccacacgga cccgtatccc tcaccgaccg acgtgcgcca agccgtcgac
      781 cccgcctggc tctacgcaat cgtgagcctg cgcgacaacg cccccatgct gcgggcctac
      841 cggatcgtcg acggcgagat cgccgaagtc caggtcgtga tcgaccggta gtgcccctcg
      901 agatcccaac tccgggaatc tcgaccgccg aggtcgggtt gtagcctgcc ggtatggccg
      961 tcgagatcaa ggtccccaac gtgttgcgcg cccaggccga gggcaaggcg agcctttcgg
     1021 tcgagggtgc gaccgtgggc gaggtgttca acaacctcgt cacccagtat ccgggcctgc
     1081 gggcgaacct tctcggtgac gacggcgggc ttcacaagtt cgtgaacgtc tacaaggacg
     1141 acgacgacat tcgttacctc gacgggctcg acaccaaggt cgccgacggc gacgtgctct
     1201 cgatccttcc cgctgtcgcc gggggatgag gcacgagaac gttctcgggc tgatcggcaa
     1261 cacaccgctc gtcggtgtgc atcaactgtc accaaatccc ggcgtcaaga tctgggccaa
     1321 gctcgaaggc cagaacccgg gcggttcgtc gaaggaccgc atcgcgttga agatggtcga
     1381 actcgccgaa cgcgacggca cgttgcaacc gggcgcgacg atcctcgaac cctcgtcggg
     1441 caacaccggt atcggcctcg cactcgtcgc gaagctccgc ggctacacgt tgcgcgtggt
     1501 catgccggag aacgtttcga tcgagcgtcg gcaactgctc gaaatcttcg gcgccgagat
     1561 cacgctctcg cccggcgaag aaggcagcaa cggcgcgatc cgcatggcgg agaagattca
     1621 cgccgacgag ccggagcgct acacgttcct gtttcagtac gggaatccgg cgaacccgct
     1681 cgcgcactac gaagcgacgg gccccgagat ttggcgcgac tgccccgagg tcgacgtgtt
     1741 ctgcgcgggc ctcggcacga gcggcacgct catgggcgtg ggcaagttct tgaaggaaca
     1801 gaatccgaac gtcaaggtgg ttgcggtcga gccgccgagt ggcgaactcg tacaaggcct
     1861 gcgcaacctc gacgacggct tcgtcccgcc gatcttcgat ccggccgtgc ttgaccgcaa
     1921 gctcatcgtc cgcccgaagg actcgatcga gtggacgcgg cgcttgctcg aagactgcgg
     1981 tgtgttcgcc ggcatctcgt cgggcgcggc gatcgcgggg gccgtgaaag cggccgcaca
     2041 gctcgagcgc ggcaccgtcg tcacgctctt gcccgacggc ggctggaaat acctttcgtc
     2101 cggcgcgtgg accgacccga tcgacgaagt cgtcgaacgc gcaacgcgca tcaactactg
     2161 gtgatcgacg acgcggtccg cgtgttgcgg cgcggcgggc tcgtggcgtt cccgaccgag
     2221 acggtctacg gactgggtgc cgacgccgcg aacgcggatg cgctccgccg gctgtatgcg
     2281 gtgaagggcc ggccgctcga ccatccggtg atcgtccatg tcgcgcgcgc ggcgcacctc
     2341 gacgagctcg cgcgcgacgt gcccgatctg gcgcacgatt tggcgcgcgc cttctggccg
     2401 ggtccgctca cgctcgtcgt gcacaagcgc gccggccgcg ttgctcccga ggccaccggt
     2461 ggtcgcgaaa ccgttggtat ccgcgtcccc gatcatccgg tcgcgctcgc attgctcgac
     2521 gcgttcgacg ggggagtggc ggcgccgtcg gcgaaccggt tcggcaaggt cagccccacc
     2581 acggccgcac acgtgcaggc cgacctcggc gccgatgtgg atctcgtgct cgacggcggt
     2641 cccgcgcgtg ttggcgtcga atcaacgatc gtggatgtca cgggggccga acccgtcatc
     2701 ctgcgcctgg gcggcgtcac cgaggcgcaa ctcgcggaga ttgccggccg cgcgctcgca
     2761 cgccgaacgg gcggtgaagt cgcggcgccg ggaacgctgc cgtcgcacta tgcgccgaac
     2821 gcgcgcgtcg tcgttgccgc gcccgatgcg gttgccgaaa tcgtgcgcga cctggaacgt
     2881 tccgggctgc gcgtggtggc gctcgacgcc ccccatgatg cgcacgagta cgcgcacgtg
     2941 ctctacgccc gcctgcgcaa cgccgacgag ccgggcgtgg atgtgatcgt ggcgcccgag
     3001 cccctcgaca ccggcggggt cggtgccgcg gtgatggatc ggcttcggcg cgccagttcc
     3061 cggtaacttc ttgctgtgga gctcacggtt ttaggctgct ccggcagcta cggcgctccc
     3121 gcgggcgggg cgtgtagtgg gtacgccgtg cgggcgggcg atgcgatcat ctggatggac
     3181 tgcgggaacg gcaccttcgc caacctgcaa caacatgtga acccggccga cctcagcgcc
     3241 gtcgtcatca cacatgcgca tcccgaccac tgcgtcgaca tcttcggctt gcacgtgttg
     3301 tacaagtacg ggatcgatcg caacatgctg ccggtgtacg cgcccgaagg tgtcgagaaa
     3361 gtgctcgaac atctcgtcgg cgactggacc gacaccttcg actggaatct cgtggccgac
     3421 ggtgacacga ccatgatcgg cgatgcgcag ctgcggttct cgcggaccga tcatccgccg
     3481 cccaccgtcg cggtggagat cgcgcatgaa ggtaagcggc tcgtctacac cgcggacacc
     3541 ggacccgagt ggagtgtcga agccttcggt cccggcgccg acttggtgct ctccgaggcg
     3601 acctacgcac acgacgacat tcgtgcgccg attcatctct ccgcgcacca ggccggggaa
     3661 gccgctcgag ccgcgcgcgc caagcgattg atgctcaccc atatttggcc gacgctcgac
     3721 ccactagcct ccgccgcgga agcatcggag acgttcggcg acacggtgac gcttgccgcg
     3781 ccgcacgccg taacgcatat ctgaggacga caccgtgacg gaacgagttt cgggccgaac
     3841 gcccgatgaa ctgcggtcgg tcgtgttcac gcgcgactac accgagatgg cgaacggatc
     3901 ggtactcgtc gaattcggac gcacgcgagt gctctgcacg gcatcggtcg aggaacgcat
     3961 cccgccgtgg ttgaagggct cgggcaaggg ttgggtcacc gcggagtact cgatgctgcc
     4021 cggctcctcg cccgaacgcg tcggccgcga agcggcgaag ggcaagcaaa gcggtcgcac
     4081 ccaggaaatt cagcggctca tcggccgttc gctccgtgcc gtcacggatc ttcgcttgat
     4141 gcccgacacg cagatcacgg ttgactgcga cgtgttgcaa gccgacggag ggacgcgcac
     4201 cgcgtcgatc tgcggcggat ggatcgcgct gcacgacgcg tgttcgcggc tcgtcggggc
     4261 aggactgttg
//
LOCUS       DASUGA010000632         2374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1555257, whole genome shotgun sequence.
ACCESSION   DASUGA010000632 DASUGA010000000
VERSION     DASUGA010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555257"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="VFX21_12500"
     CDS             <1..968
                     /locus_tag="VFX21_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010352201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5096835.1"
                     /translation="EYGGRDASLWEWLIFEEEYYRAGAPQRVTQNGIFLLAPTVFEFG
                     TDRQRAEILPKMASGEQTWCQGWSEPNAGSDLAGIQSKAVRDDARGGWRLSGQKTWTT
                     RGAFCTHLFGLFRSNPDAERHRGLTYFLVDLATPGVTVRGVERLDGDEGFAEVFFDDA
                     FVSDDDILGAANKGWEVAMATTGSERGLTLRSPGRFLATAQRLIDLYHARNDDPELRD
                     RLVDAWIDAEAYRWQTFATVSRIVDGERTGAESSLVKLFWSELDVRLHELALELLGPH
                     AELLDGDDAAWMKGYEFALAGPIYAGTNEIQRNVVAERVLGLPRR"
     gene            965..1855
                     /locus_tag="VFX21_12505"
     CDS             965..1855
                     /locus_tag="VFX21_12505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007951377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5096836.1"
                     /translation="MRFAFTDEQLEFRDGLRALLEKECTPAHLRDAWTNESGRVPGLW
                     EQLVDMGVVALLAPEADGGLGLTFVDLVLLLEETGRHAVPEPIVETAAIGVPLLRATG
                     TVAAGEELVPWADTAGVIVTAAGRFDSTTAALTPHASVDGARRLFTVAGTGAEVDLDA
                     VFVRGALGTAAQQCGLADRMIELTVGYVKERQQFGVPVGSFQAVKHHLANARLALEFA
                     RPLVYRAAVTLDPVHVSMAKAKADGAALTAARVALQCHGAIGYTTEYDLHLFMKRAWA
                     LARTWGDARAHRNRVATAVL"
     gene            1866..>2374
                     /locus_tag="VFX21_12510"
     CDS             1866..>2374
                     /locus_tag="VFX21_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX5096837.1"
                     /translation="MPEAYIVDAVRAPVGRRNGGLAQVHPADLGAHSIRALMDRVDVD
                     PAAVEDVVFGCVDTIGPQAGDIARTCWLVAGLPDEVPGTTVDRQCGSSQQAVHFAAQA
                     VMAGVNDLVVAGGVQNMSAIPITAAMLAGEPFGFENPFSGSPGWEARYGTQEVSQFRS
                     AEMIAEKWD"
BASE COUNT          347 a          841 c          838 g          348 t
ORIGIN      
        1 aggagtacgg cggccgcgac gcatccctgt gggagtggct catcttcgaa gaggagtact
       61 accgcgcggg cgcgccgcaa cgcgtgaccc agaacggcat cttcctgctc gcgccgacgg
      121 tcttcgagtt cggcaccgac cgccagcgcg ccgagatcct gccgaagatg gcgtccggcg
      181 agcagacgtg gtgccagggt tggtcggaac cgaacgcggg ctccgacctc gccggcatcc
      241 agtcgaaggc ggtgcgcgac gacgcgcgcg gcggctggcg cctgtcggga cagaagacct
      301 ggacgacgcg cggcgcgttc tgcacgcacc tcttcggctt gttccgtagc aaccccgacg
      361 ccgaacgcca ccgcgggctc acctacttcc tcgtcgatct cgcgacgccg ggtgtgacgg
      421 tgcgcggcgt cgagcgtctc gacggcgacg aaggcttcgc cgaagtgttc ttcgacgacg
      481 cgttcgtgag cgacgacgac atcctcggcg cagcgaacaa gggctgggaa gtcgcgatgg
      541 cgacgacggg ctccgagcgc ggcctgacgc tgcgcagccc cggccgtttc ctcgcgaccg
      601 cgcaacggtt gatcgacctg taccacgcgc gcaacgacga tccggagctg cgcgaccggt
      661 tggtcgatgc gtggatcgac gccgaggcgt accgctggca gaccttcgcg acggtctcgc
      721 gcatcgtcga cggcgaacgc accggcgcgg agtcgagcct ggtgaagttg ttctggtcgg
      781 agctcgacgt gcgcctgcac gagctcgcgc tggagctgct cgggccgcac gccgagttgc
      841 tcgacggtga cgacgccgcg tggatgaagg gctacgagtt cgcgctcgcg ggcccgatct
      901 acgccggcac caacgagatc caacgcaacg tcgtcgccga acgcgtcctc gggctgccgc
      961 gccgatgaga ttcgcgttca ccgacgaaca gctcgagttc cgcgacgggt tgcgcgcact
     1021 gctcgagaag gagtgcacac cggcccacct ccgagacgcg tggacgaacg agtccgggcg
     1081 cgtgcccggt ctgtgggaac aactcgtcga tatgggtgtc gtcgcgctgc tcgcgccgga
     1141 agccgacggc gggctcgggc tcacattcgt cgacctcgtc ttgctgctgg aggagaccgg
     1201 ccgccacgcc gtccccgagc cgatcgtcga gacggccgcc atcggcgttc cgctgttgcg
     1261 cgcgacgggc actgtcgctg cgggcgaaga gctcgtgccg tgggccgaca ccgccggcgt
     1321 catcgtgacc gcggccggcc gcttcgacag cacgaccgcc gcgctgacgc cccacgcgtc
     1381 ggtcgacggc gcgcgccggt tgttcaccgt cgcgggcacg ggtgccgagg tcgacctcga
     1441 cgcggtcttt gtgcgcggcg cgctcggcac cgccgcgcaa cagtgcggtc tcgccgaccg
     1501 catgatcgag ctcacggtcg gttacgtgaa ggaacggcag cagttcggcg tccccgtcgg
     1561 ctcgttccag gcggtgaagc accacctcgc gaacgcgcgc ctcgcactcg agttcgcgcg
     1621 gccgttggtc taccgcgccg cagtcacgct cgaccccgtg cacgtcagca tggccaaggc
     1681 caaggccgac ggcgccgcgc tcaccgcggc ccgggtggca ctgcagtgcc acggcgcgat
     1741 cggctacacg accgagtacg acctgcacct cttcatgaag cgcgcgtggg cgctcgcccg
     1801 cacgtggggc gacgcgcgcg cccaccggaa ccgcgtcgcg acagccgtgc tttaggctgc
     1861 ggctcatgcc cgaggcctac atcgtcgacg ccgtccgtgc tcccgtcggg aggcgcaacg
     1921 gcgggctcgc gcaggtccac ccggcggacc tcggcgcgca ctcgatccgg gcgctgatgg
     1981 accgggtcga cgtcgacccc gccgccgtcg aggacgtcgt cttcgggtgt gtcgacacga
     2041 tcggccccca ggccggcgac atcgcgcgca cgtgctggct cgtcgcgggc ctccccgacg
     2101 aggtgccggg caccaccgtc gaccgccagt gcggttcgtc ccagcaggcc gtgcacttcg
     2161 cggcgcaggc cgtcatggcc ggcgtcaacg acctcgtggt cgcgggcggt gtgcagaaca
     2221 tgagcgcgat cccgatcacc gctgcgatgc tcgcgggcga gccgttcggc ttcgagaacc
     2281 cgttctcggg ctcacccggc tgggaagcgc gttacggcac gcaggaagtg tcgcagttcc
     2341 ggtcggccga gatgatcgcc gagaagtggg acat
//
LOCUS       DASUGA010000633         3441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1311099, whole genome shotgun sequence.
ACCESSION   DASUGA010000633 DASUGA010000000
VERSION     DASUGA010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311099"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..598
                     /locus_tag="VFX21_12515"
     CDS             86..598
                     /locus_tag="VFX21_12515"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX5096838.1"
                     /translation="MPPRSKLLAVAGIALVALGAIAFMAWRFNAADDGDAGFALAPTT
                     RAVAPFSMFDEARVAIDKDCKRLLIARSQAQRVQGLRDVTDLAPYDGMLFVFPSETTA
                     RFTMANTPMALDITFLDANGVPVDSKTMEPCPDGGDASCPQHASDRKYRYALEVPAGG
                     SGGALGTCSA"
     gene            735..923
                     /locus_tag="VFX21_12520"
     CDS             735..923
                     /locus_tag="VFX21_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096839.1"
                     /translation="MKICEHCGDWSGNAYERCVACGAQRFFPYSPPVQYWHRWDVHTE
                     TVTTSLARLPVEPVSPRG"
     gene            1068..1403
                     /gene="rpsF"
                     /locus_tag="VFX21_12525"
     CDS             1068..1403
                     /gene="rpsF"
                     /locus_tag="VFX21_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005467069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX5096840.1"
                     /translation="MRPYEVMIIIDADLEEETIRASVERWLKLIEERGAERGHVDYWG
                     KRRLAYEIKHKAEGYYVVLQARAEPEAMVELSRVLSLADEVIRHKVLRIPEKVYGPPQ
                     AAGTAAEHA"
     gene            1415..1900
                     /gene="ssb"
                     /locus_tag="VFX21_12530"
     CDS             1415..1900
                     /gene="ssb"
                     /locus_tag="VFX21_12530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX5096841.1"
                     /translation="MATNLNNVTVVGNITRDPEMRFTANGRSTTSFGLAVNRSWRNQQ
                     TNEWEERTSFFNVVTWGNLADNVGSSLKKGMRVLVTGRLEQRSWETEQGDKRSVVEIV
                     ADEVGPSLLFATAEVHRIERSGPDGGGGRPQQRQTAPANAGGGGGDNNNNYDDYGEEP
                     F"
     gene            1912..2160
                     /gene="rpsR"
                     /locus_tag="VFX21_12535"
                     /pseudo
     CDS             1912..2160
                     /gene="rpsR"
                     /locus_tag="VFX21_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005179663.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
     gene            2370..2819
                     /gene="rplI"
                     /locus_tag="VFX21_12540"
     CDS             2370..2819
                     /gene="rplI"
                     /locus_tag="VFX21_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX5096842.1"
                     /translation="MKILLREDVENLGLKGEIVEVADGYARNFLVPRGLALRATPGHQ
                     KQADAMTRNREARGRREREAAQELAAKFDGRVVTIKARAGGEGRLFGSVTNVDIADAV
                     LSQTGAEIDRRKITLDEPLKEIGGVDLQVRLHPDVVANLRVEVEASS"
     gene            3042..>3441
                     /locus_tag="VFX21_12545"
     CDS             3042..>3441
                     /locus_tag="VFX21_12545"
                     /inference="COORDINATES: protein motif:HMM:NF012974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HEX5096843.1"
                     /translation="MYSVRDEAQVRGRIPPHNLEAEESLLGAMMLSREALNAAIEARV
                     EAHDFYKPAHGYIFEAAYALHSRGEPVDPVTVAEELRRASQLDDLGGRQTLLRIQAAT
                     PASANASQYAQIVAELAMLRRLIETAGDIQE"
BASE COUNT          668 a         1104 c         1147 g          522 t
ORIGIN      
        1 taccacatga ccgaccagat ggccgacgcc ctcggcctct acggcgggac cacgcccgaa
       61 cccgaactga cgcaataatc acggcgtgcc tccgcgttcg aagctgctcg cggtggccgg
      121 gattgctctg gttgcgctcg gtgcgatcgc gttcatggcc tggcgcttca acgcggccga
      181 cgatggcgac gccggcttcg cgctcgcgcc gacgacgcgg gctgtcgcgc cgttctccat
      241 gttcgacgag gcccgggtcg caatcgacaa ggactgcaag cggctgttga tcgcacgatc
      301 acaggcgcag cgcgtgcagg ggctgcgcga cgtcaccgat ctcgcgccgt acgacgggat
      361 gctcttcgtg ttcccgtccg agacgacggc gcgcttcacg atggcgaaca cgccgatggc
      421 actcgacatc acgttcctcg acgcgaacgg cgtgccggtc gattcgaaga cgatggaacc
      481 gtgtcccgac ggcggcgacg cgtcgtgtcc gcagcacgcg agtgacagga aataccgcta
      541 tgcactcgaa gttccagccg gtgggtccgg tggcgcactg ggtacttgtt ccgcatagcg
      601 gaccggatcg ccagcggtcc cgctaatcgc cgatgggtcc aattccacgg ccgttgcctc
      661 gaaagtcgcg agaattcgga ccaagatcgc tctgttctgc gttcggcgct cctgcgccgg
      721 gaaggagaac aggcgtgaag atctgcgagc actgcggtga ctggagcggg aacgcatacg
      781 aacgttgcgt ggcgtgcggc gcgcaacgtt tcttcccgta cagcccgccg gtgcagtact
      841 ggcaccgctg ggacgtgcac accgagaccg tcacgacgtc actggcgcgc ctgcccgtag
      901 aacctgtctc accccgcggt tagggtcttc ctcgataggt ccaagtcggg tgatctgcta
      961 gctttcaccg gttcacaacc cacgccctgc cccgaagccg ccggaaaacc cgggtgcgcc
     1021 aggggcccgc aacgacctgc gttgcggtcc gaaggaggta ctggccgatg cggccgtacg
     1081 aagtgatgat catcatcgac gcggacctgg aagaggagac gatccgggca tccgtcgagc
     1141 ggtggctcaa gttgatcgag gaacgcggcg ccgagcgcgg ccacgtcgat tactggggca
     1201 agcgccgcct cgcctacgag atcaagcaca aggccgaggg ctactacgtc gtcctccagg
     1261 cccgcgctga acccgaggcg atggtcgagt tgtcccgggt gctctccctg gcagacgaag
     1321 tcatccgcca caaggtgcta cgcattccgg agaaggtcta cggacctccc caggctgcag
     1381 gcacagcggc ggaacacgcg tagggagcag cgatatggca acgaacctca acaacgtcac
     1441 ggtcgtcgga aacatcacgc gcgaccccga gatgcgcttt accgccaacg gtcgttcgac
     1501 gacgagcttc gggctcgccg tcaaccgttc gtggcgcaac cagcaaacga acgaatggga
     1561 agagcgcacg tcgttcttca acgtcgtcac gtggggcaac ctcgccgaca acgtgggttc
     1621 gtcgctgaaa aagggaatgc gcgtgctggt caccggccgg ctcgaacagc gcagctggga
     1681 gaccgagcag ggcgacaagc ggtcggtggt cgagatcgtc gccgacgaag tcgggccgag
     1741 cttgctgttc gcgaccgccg aggtgcaccg catcgagcgc agcggtcctg acggtggcgg
     1801 cggccgtccg cagcagcgcc agaccgcacc ggccaacgcc ggtggcggcg gcggtgacaa
     1861 caacaacaat tacgacgact acggcgaaga acccttctag gaaaacgagc tatggcacaa
     1921 cgtcgcgaga agaaggcgcc ccccggcaag gatcggcgcc cgatcaagaa gaaaacgtcg
     1981 atcctgacga ccgaaaacat cgactggatc gactacaagg acgtgaactt gctgcggcgg
     2041 ttcatgtcgg aacgggccaa gatccgtgct cgccgcgtga ccggcaacag ccagcagcag
     2101 caggttgcgg tcgcgcgtgc gatccgagtg gcgcgcgaga tggcgttgct gccctacagc
     2161 gtgcgccagg tcacgcagcg gtcgaagggc cgtcgcgatc gcgatcgcga ccgtgacgac
     2221 cggccgatgg ttgccgagga cgagaacgcc gaggcgttgg gcgccgaggg cgaagagctc
     2281 gacgaactcg aaggcaccga agagttcggt ggcgagacgt tcgagtctgg cgacgacgag
     2341 gacgacgtgc tcgcggcgga gggcgcggaa tgaagattct cttgcgcgaa gacgtcgaga
     2401 acctcggcct gaagggcgag atcgtcgaag tcgctgacgg ctacgcccgc aacttcttgg
     2461 tgccgcgtgg gctcgcgttg cgcgcgacgc ccgggcacca gaagcaagcc gacgcgatga
     2521 cacgcaatcg tgaagctcgc ggccggcgcg aacgcgaggc cgcgcaggaa ctggccgcga
     2581 agttcgacgg cagggtggtg acgatcaagg cgcgagcggg gggcgaaggc cggctgttcg
     2641 gttcggtcac caacgtcgat attgccgacg cggtgctcag ccagaccggc gcggagatcg
     2701 accgccgcaa gatcaccctc gacgaaccct tgaaggagat cggtggcgtc gacctgcagg
     2761 ttcgcctgca ccccgacgtc gtcgcgaacc ttcgggtgga ggtcgaagcc tcgagttgag
     2821 ggtttccaca ccctctggac cagcccgatc cgagcgttac ccacaaggct gtggataacg
     2881 cgcgcccgga gatccccgtg actgtccgac ccttgggcga caatccccaa gggtgtggat
     2941 gacgcaaccc gtcgtccaca cacaagtcac agggttaatc ccctatcgac ccacaggcgg
     3001 tccgtgagat cttcactggc cgctgttccg gggagatgtc gttgtattcc gttcgagacg
     3061 aagcacaggt tcggggccgg atcccacccc acaacctcga agccgaggag tcgctgctcg
     3121 gcgccatgat gctctcgcgc gaagcgttga acgccgcgat cgaggcgcgc gtcgaagcgc
     3181 acgactttta caagccggcg cacggctaca tcttcgaagc ggcgtacgcg ttgcacagcc
     3241 gcggcgaacc tgtcgacccg gtcaccgtcg cggaagagct gcggcgcgcg tcgcagctcg
     3301 acgatctcgg cggccgtcag accctgttgc ggattcaagc cgcgacgcct gcgtcggcga
     3361 acgcgtcgca gtacgcgcag atcgtcgccg aactcgcgat gctgcgccgc ctcatcgaga
     3421 ccgcaggcga cattcaagag a
//
LOCUS       DASUGA010000634         4134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1311282, whole genome shotgun sequence.
ACCESSION   DASUGA010000634 DASUGA010000000
VERSION     DASUGA010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311282"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFX21_12550"
     CDS             complement(<1..296)
                     /locus_tag="VFX21_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096844.1"
                     /translation="MRSREAVFLFDEGPGIGLGHRRRCEALANELQLLDWSTRLVPLA
                     GANRVAAQIVVVDSYIARADEMEIDAGLLVALDDVARDLDVDVLIDPSPGADASV"
     gene            complement(293..1336)
                     /locus_tag="VFX21_12555"
     CDS             complement(293..1336)
                     /locus_tag="VFX21_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEX5096845.1"
                     /translation="MSVFIGDREVGPGRPSYVIAEAGANHDRDLDVARRLIDVACEAG
                     ADAVKFQTYSGRALYSTKTPRFEYLGELGAKPAHELLDDLALPREWQPILAAHCRDAG
                     IEFLSSPFDRDAVDELDDLDVAAFKIASFELVDLPFIEYVGSKKRPIILSTGMATLGE
                     IEEALTAARSGGARELCLLQCASLYPSPPKVMNLRTIPALQQAFGVPVGLSDHTLGVH
                     IAPAAVALGAGLVEKHFTLDRSRPGPDHPFAIEPTELTALVAHIRDVEAALGDGVKRG
                     PSDEEMVEMYTKARRSVVAACAIPAGTLITREMLTVKRPGFGIAPKFIDALAGRVARV
                     DIEDDEVVTWEML"
     gene            complement(1344..2546)
                     /locus_tag="VFX21_12560"
     CDS             complement(1344..2546)
                     /locus_tag="VFX21_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096846.1"
                     /translation="MTAEEIPGSGCDEFDRARALLAEGREAEALDWFEVASTSAQQPE
                     VRASACAFAAGILLAKAQPFEVPVWADVLRVAGGDGALADLLEAASYLQLGDVVAARG
                     LLDTVGTPSDPWFPCSPTTVRVMRAHVHYLDGERDRARDEVLEIFADDPDSLELWDAF
                     ARLCAETDFDPTAIVAAIPDDRVLPVLAGLRSSEAAGVDRIADLIWQRDPGDARVLAL
                     VPSFASKLESLRCLEWAARMRAAGMGRSCPLVGRAEDIRVDARERVRAAALVHVSFGD
                     RRGRESLELAVAALEDREIAPALHEVWTLASTLTDSVVVAAAVSSRRSLIVAAALYEG
                     GASDEAYAVLVHGLAMQDAAELTTDDVIALLPVTALEGLAEQAELRGEEDVAGILEAV
                     AVVAGSQG"
     gene            2783..3130
                     /locus_tag="VFX21_12565"
     CDS             2783..3130
                     /locus_tag="VFX21_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HEX5096847.1"
                     /translation="MIDLAMRVIQQSLDGLAARQRIIAENIANVDTPGFHAGRVSFED
                     NLRTAIDKGDSPESVGISTQRSTDPTRPNGNNVNIDDENISLIETGLRYQLAIDSMNA
                     KFRILRTSMRRDS"
     gene            3130..3549
                     /locus_tag="VFX21_12570"
     CDS             3130..3549
                     /locus_tag="VFX21_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HEX5096848.1"
                     /translation="MSIFPVLHTAGSGLRVDRVWMDSVADNIANINTIKPYDQPAFQA
                     RYVVAQSVRSDDNDPPGVGGGAVVKEIQYGDAEGRLRYEPDHPFANEEGIVRYPDIDL
                     GTQMTSLIQAQRAYELNLAVVDRARDSYLQALQLNGR"
     gene            3554..3868
                     /gene="fliE"
                     /locus_tag="VFX21_12575"
     CDS             3554..3868
                     /gene="fliE"
                     /locus_tag="VFX21_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015772294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEX5096849.1"
                     /translation="MAIPAVGSLGALSGLSPVETAKKAAKPAGEGQGDFGNVVVNALE
                     SLNALQSKSDNLAVKAATGDLSDVHDYMIASTEASLATEFTVAIRNRAVEAFNEIMRM
                     QI"
     gene            3868..>4134
                     /locus_tag="VFX21_12580"
     CDS             3868..>4134
                     /locus_tag="VFX21_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HEX5096850.1"
                     /translation="MAALDMDRIRAQGKRFADGFTPGQKAMTLLGVVAVVIASMMFMR
                     WAGSPTWGTLYTGLESADAAKVTDELTSQGVKFKLEDGGHTVLVP"
BASE COUNT          717 a         1445 c         1395 g          577 t
ORIGIN      
        1 accgacgcgt ccgcgcccgg tgacgggtcg atcagcacgt cgacgtcgag atcacgagcc
       61 acatcgtcga gcgcgacgag caagcccgcg tcgatctcca tctcgtcggc gcgcgcgatg
      121 taggaatcga cgacgacgat ctgcgccgcg acccgattcg cgcccgcgag cggaacgaga
      181 cgggtcgacc agtcgagcag ctggagctcg ttggcgagcg cttcacaacg ccgccggtgc
      241 ccgagcccga tacccggacc ttcgtcgaag aggaataccg cctcgcgcga cctcacagca
      301 tctcccaggt gacgacttcg tcgtcctcga tgtcgacgcg ggcgacgcgg ccggcgagcg
      361 cgtcgatgaa cttcggcgcg atcccgaagc ccgggcgctt cacggtcagc atctcgcgcg
      421 tgatgagcgt gccggccggg atcgcgcacg cggcgacgac ggaacggcgc gccttcgtgt
      481 acatctcgac catctcttca tcactcggcc cgcgtttgac gccgtcgccc aacgcggctt
      541 cgacgtcgcg gatgtgcgcg acgagcgcgg tgagctcggt gggctcgatc gcgaacgggt
      601 gatcggggcc gggacgcgac cggtcgagcg tgaagtgctt ctcgacgagg ccggcaccga
      661 gcgcgacggc cgccggcgct atgtgcacgc cgagcgtgtg gtcactcagc ccgaccggga
      721 cgccgaaggc ctgctgcagc gcggggatcg tgcgcaggtt catcaccttc ggtggcgacg
      781 ggtacagcga tgcgcactgc aagaggcaga gctcgcgcgc gccgccactc cgggcggccg
      841 tcagcgcctc ttcgatctca ccgagcgtcg ccatgccggt cgacaggatg atcggacgct
      901 tcttcgatcc gacgtactcg atgaacggca agtcgaccag ctcgaaggac gcgatcttga
      961 acgcggcgac gtcgaggtcg tccaactcgt cgaccgcgtc acggtcgaac ggactcgaca
     1021 ggaactcgat gccggcgtca cggcagtgcg ccgcgaggat cggctgccac tcgcgcggca
     1081 gcgcgagatc gtcgagcaac tcgtgcgccg gcttggcgcc gagctcaccg agatactcga
     1141 agcgcggggt cttcgtcgag tagagcgcgc gccccgaata cgtctggaac ttcaccgcgt
     1201 cggcaccggc ttcgcacgcg acgtcgatca gacggcgggc gacgtcgagg tcgcgatcgt
     1261 ggttcgcgcc ggcctcggcg atgacgtagc tcggacggcc ggggccgacc tcacgatcgc
     1321 cgatgaagac cgacaacgca gtgttatccc tgcgacccgg caacgaccgc gactgcttcg
     1381 aggatgcccg ccacgtcttc ctcgccgcgc agttcggctt gctcggcgag tccttcgagc
     1441 gcggtgaccg gcaggagcgc gatcacgtcg tccgtcgtca gttcggcggc gtcctgcatc
     1501 gcgaggccgt ggaccagtac cgcgtaggcc tcgtcggatg cgccgccttc gtagagggcg
     1561 gcggcaacga tcagcgagcg gcgtgagctc accgccgccg cgacgacgac cgagtcggtg
     1621 agggtcgacg ccaacgtcca cacctcgtgc aacgccggtg cgatctcgcg gtcttcgagc
     1681 gccgcgacag ccagttcgag tgactcgcgc ccgcgccggt cgccgaagga cacgtgcacc
     1741 agcgcggcgg cccgcacgcg ttcgcgggcg tcgactcgaa tgtcctcggc ccggcccacg
     1801 agcgggcacg aacggcccat gccggcggcc cgcatgcgcg ccgcccactc gaggcaacgc
     1861 aggctctcga gcttcgatgc gaacgacggc accagcgcca acacccgcgc gtcacccggg
     1921 tcgcgctgcc agatgaggtc ggcgatgcga tcgacgccgg cggcctccga cgagcgcagg
     1981 ccggccagga cgggcaagac ccggtcgtcg gggatcgcgg cgacgatcgc ggtcgggtcg
     2041 aagtcggtct ccgcacacag ccgggcgaac gcgtcccaca actcgagcga gtcggggtcg
     2101 tcggcgaaga tctccaacac ctcgtcgcgc gcccggtctc gctcgccgtc gaggtagtgc
     2161 acgtgcgcgc gcatcacccg gaccgtcgtc ggcgagcacg ggaaccacgg gtcggatggt
     2221 gtcccgaccg tgtccaggag cccgcgcgcg gcaacgacgt caccgagttg caggtacgac
     2281 gcggcttcga gcaggtcggc gagcgcgccg tcaccgccgg ccacgcgcag cacgtcggcc
     2341 cacaccggca cttcgaaggg ctgggccttc gccaacagga tgccggccgc gaaggcgcac
     2401 gccgaggccc gtacttccgg ctgttgcgca ctcgtcgagg cgacttcgaa ccagtcgagc
     2461 gcttcggcct cacggccctc ggccagcagg gcgcgcgcac ggtcgaactc gtcgcagccg
     2521 gaaccgggaa tctcctcagc ggtcacgaca cctcatcggc agcgggcgcc ggagttgtag
     2581 agggccgcgg cacgcgtgag ccgccaacgc ggacggtgac aaagacaagg tcagaccgct
     2641 caactcccgg aagaccccgc cgatgttgtg atcgctaggg ccatcgggcg gggcacggag
     2701 cgcctcgccg acgcgatccc acggacggac acccactgaa ggggtctgac gtgcaccaag
     2761 gacgtgtgcc gaggcggtca cgatgatcga cctcgcaatg cgcgtcatcc agcagtcgct
     2821 cgatggcctc gccgcgcgcc agcgcatcat cgccgagaac attgccaacg tcgacacccc
     2881 cggcttccac gccgggcgcg tgtcgttcga ggacaacctc cgcaccgcga tcgacaaggg
     2941 cgactcgccg gagtcggtcg gcatctcgac gcagcgctcc accgatccga cgcgacccaa
     3001 cggcaacaac gtcaacatcg acgacgagaa catctcgctc atcgagacgg gtctgcgcta
     3061 ccagctcgcg atcgactcga tgaacgcgaa gttccgcatc ctgcgcacat cgatgaggcg
     3121 ggatagctga tgtcgatctt ccctgtgctg cacaccgcgg gcagcggcct gcgcgtcgac
     3181 cgggtctgga tggactccgt cgccgacaac atcgccaaca tcaacacgat caagccgtac
     3241 gaccagccgg cgttccaggc ccgatacgtc gtcgcgcagt ccgtgcgcag cgacgacaac
     3301 gacccgcccg gtgtcggcgg cggcgcggtc gtcaaggaga tccagtacgg cgacgccgag
     3361 ggtcgtctgc gctacgagcc cgaccacccg ttcgccaacg aagaaggcat cgtccgctac
     3421 cccgacatcg acctcgggac gcagatgacg agcctgatcc aggcccaacg cgcctacgag
     3481 ctgaacctcg cggtcgtcga ccgggcgcgc gactcgtacc tccaagccct ccagttgaac
     3541 ggacggtagc tagatggcga tccctgcagt tggttcgttg ggcgcgctca gcgggttgtc
     3601 acccgtcgag accgcgaaga aggccgccaa gcccgcgggc gagggccagg gcgacttcgg
     3661 caacgtcgtc gtcaacgcgc tcgagagcct caacgcgctg cagagcaagt cggacaacct
     3721 cgcggtgaag gctgcgacgg gcgatctgag cgacgtgcac gactacatga tcgcttcgac
     3781 cgaggcgtcg ctcgcgaccg agttcacggt cgcgatccgc aaccgcgcgg ttgaggcctt
     3841 caacgagatc atgaggatgc agatctgatg gcggcgctcg acatggatcg gatccgcgcg
     3901 cagggcaagc gcttcgccga cgggttcaca cccggccaga aggcgatgac gctgctcggt
     3961 gtcgtcgcgg tcgtgatcgc gtcgatgatg ttcatgcgct gggccggttc gccgacctgg
     4021 ggcacgctgt acacgggcct cgagtccgcg gacgcggcca aggtgaccga cgaactgacc
     4081 tcgcagggcg tgaagttcaa gctcgaagac ggcggccaca ccgtgctcgt gcca
//
LOCUS       DASUGA010000635         4668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1485468, whole genome shotgun sequence.
ACCESSION   DASUGA010000635 DASUGA010000000
VERSION     DASUGA010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485468"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VFX21_12585"
     CDS             complement(<1..406)
                     /locus_tag="VFX21_12585"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5096851.1"
                     /translation="MNATPDVLAMHADAQPDKVAVIVDDSGGARPSTTTFAELNTLVN
                     RLAHGITAAGTQARERLVWCGPNSLEVLATIHASRKLNLVAVPLSYRFNAEEMQYVID
                     NSDATIVVVDAEQAELVASVRSELPKVRKVVVF"
     gene            443..2056
                     /locus_tag="VFX21_12590"
     CDS             443..2056
                     /locus_tag="VFX21_12590"
                     /inference="COORDINATES: protein motif:HMM:NF019193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HEX5096852.1"
                     /translation="MSRAFRRRGVVEGFYGTPWSHDARLELLTFLGARDMNAYVYAPK
                     DDAKHRTRWREPYDATESKQFEELAAHASGCGIRFGFAISPGLDISYESTDDRVALLA
                     KLRPLLDGGVPWFCLLLDDIPMQAGLAPRQADLAAWLLDALRTENADVALTLCPTEYV
                     GTRPSEYLHALGAALPADVDVMWTGPTVCSPTITVADAREWTDALGGRPPLLWDNYPV
                     NDGTMGTCLHIGPYEGRDPELADIVGGVLCNPMEYAYASKIPLATAAAFLRDPDAYDA
                     DDAWVDAISDVGGDRAPMLTVLAHACADSALREPDALILNRALDVVEEEILFPGWIDD
                     LAVIAAIFRATKALPDAFAAADGDPLARDLAPWVGAARREAEAGLAALRVMQAVRPVA
                     VIDDDGRGRAATIDAERAMQLLFMMMFQWSAARSNKKSVFGPRFAIYPAVVQLADGSQ
                     GVDPTLAVREDANAVDRCCRLALAVYDAWRTAPGGDLRVLVDGEERAVAADGTFDGRG
                     TLTLVRAGEWCTRLDHPLPFNEPRLAEKE"
     gene            2053..2787
                     /locus_tag="VFX21_12595"
     CDS             2053..2787
                     /locus_tag="VFX21_12595"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5096853.1"
                     /translation="MSTEVGIDVTVVMPAYNEAEILEQSVGDVCNGLRDRGVCFELLV
                     VENGSTDNTLALAHELAEKYDEVRAEHSDAPDYGRALRHGLLLAQGAAVVNFDADYYD
                     LDFLQAAVAKVTAEGGPAIVVGSKRSEGSNDTRPFLRKTVTWTFSTILRVVFGLKVSD
                     THGMKAMRRVAVVPYARACQFGQDLFDTELILRCERAGLPTSEIPVPVIELRPARSSI
                     LRRVPRTILGLSKLRVALWRDRNRVV"
     gene            2784..3869
                     /locus_tag="VFX21_12600"
     CDS             2784..3869
                     /locus_tag="VFX21_12600"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5096854.1"
                     /translation="MTCLTAARIVTGDGGVSASPAWVVVEDGRIVASGSGAAPQPAED
                     LGDALLAPGFVDLQVNGTGTLDFATASIDELRAAIDSLVAGGCTSLLPTLCSAPLDTY
                     LPALQRIADLRATCDAVLGVHLEGPFLGGAPGAHPTDLVRPVDLPWLVGLLDATGDLI
                     RLVTLAPEADPGFAATRALTQRGIAVALGHSIAGYDEARAGADAGARMVTHCFNGMGP
                     LHHRAPGLPGAALDDRRLVPSVICDLVHVHPAVLRMVTTVRPDAVAVTDAVGGDLVVG
                     DGGAAYLADGTLAGARVTMAEVVQNLAGIGLAPGAAVRMATGNGAKAIGATDRGRIAP
                     GCRADFVTLDPDTLAVREVWVGGTRRM"
     gene            3879..4598
                     /locus_tag="VFX21_12605"
     CDS             3879..4598
                     /locus_tag="VFX21_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017240777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX5096855.1"
                     /translation="MARVALFVTCVADQLFPDTAWSTVRLLEAAGDTVEFPEAQTCCG
                     QPALAAGERDAVPRLAHHFLDTFAGYDAVVAPSGSCTAMVHHWYKELVPERAAEVEAL
                     AARTYELTQYLVDVRGLVDVGARVDATVTVHDACHGLRTLGLAHPTRRLLAAAGATIV
                     ELTEPETCCGFGGVFAAEYPEVSTKLADAKLADARATSAQYLVSTDLACLMHLEGRAR
                     RVRSGPRAIHVADLLAPGLPA"
BASE COUNT          633 a         1680 c         1626 g          729 t
ORIGIN      
        1 cgaacacgac gactttgcgg accttcggca gctcgctgcg caccgacgcg accagctcgg
       61 cctgctcggc gtcgaccacc acgatcgtcg cgtcggagtt gtcgatgacg tactgcatct
      121 cctcggcgtt gaaccggtac gacagcggca ccgcgacgag gttgagcttg cgcgaggcgt
      181 gaatcgtcgc cagcacttcc agcgagttcg gcccgcacca tacgagccgc tcgcgggcct
      241 gcgtgccggc cgcggtgatg ccgtgcgcga gccggttcac cagtgtgttc agctcggcga
      301 aggtggtggt ggacggacgg gcgccgccgc tgtcgtcgac gatcacggca accttgtcgg
      361 gctgcgcatc cgcgtgcatt gcgaggacgt caggcgtggc gttcatcggt tccccccggt
      421 cggggccgtt accctactca ccgtgtccag ggcgttccgc cggcgtggcg tcgtcgaagg
      481 cttctacggc acgccctggt cgcacgacgc ccggctcgag ctgctgacgt tcctgggcgc
      541 gcgcgacatg aacgcctacg tctacgcgcc caaggacgac gcgaagcacc gcacccgctg
      601 gcgcgagccg tacgacgcga ccgagtcgaa gcagttcgag gagttggccg cgcacgcgtc
      661 cggctgcggc atccggttcg ggttcgcgat ctcgcccggc ctcgacatct cctacgagtc
      721 gactgacgac cgcgttgcgc tgctcgccaa gttgcgcccg ttgctcgacg gcggcgtccc
      781 ctggttctgc ctgctgctcg acgacatccc gatgcaggcc ggcctcgcgc cccgccaggc
      841 cgacttggct gcgtggttgc tcgacgcgtt gcgcaccgag aacgccgacg tcgcgctcac
      901 tctgtgcccg accgagtatg tcggcactcg cccgtcggag tacctccatg cgctcggcgc
      961 cgcgttgccc gccgacgtcg acgtcatgtg gacgggcccg acggtgtgct cgccgaccat
     1021 caccgtcgcc gacgcccgcg aatggaccga cgcactcggc gggcgtccgc cgctgttgtg
     1081 ggacaactat ccggtcaacg acggcacgat gggtacctgt ttgcacatcg gtccgtacga
     1141 agggcgcgac cccgaactgg ccgacatcgt cggtggcgtg ctgtgcaacc cgatggagta
     1201 cgcgtacgcg tcgaagatcc cgctcgccac cgcggccgcg ttcttgcggg accccgacgc
     1261 gtatgacgcc gacgacgcgt gggtcgacgc gatcagcgac gtcggcggcg accgggcgcc
     1321 gatgctgacc gtgcttgcac acgcgtgcgc cgacagcgcg ttgcgcgagc ccgatgcgtt
     1381 gatcctcaac cgggcgctcg acgtcgtcga ggaagagatc ctcttcccgg gctggatcga
     1441 cgacctcgcg gtcatcgccg cgatcttccg ggcgacgaaa gcgctgcccg acgcgtttgc
     1501 ggcggccgac ggcgatccgc tcgcccgcga cctcgcaccg tgggtcggcg cggcgcggcg
     1561 ggaagccgag gccgggctcg ccgcgctgcg ggtcatgcag gccgtccggc cggttgcggt
     1621 gatcgacgac gacggtcgag gccgggccgc gacgatcgac gccgaacgcg cgatgcagct
     1681 cctcttcatg atgatgttcc agtggagcgc cgcccgctcg aacaagaagt cggtcttcgg
     1741 tccccgcttt gcgatctatc cggcggtggt ccagctcgct gacggcagcc agggcgtcga
     1801 ccccacgctc gcggtgcgcg aggacgccaa cgcggtcgac cggtgctgcc gcctcgccct
     1861 cgccgtgtac gacgcctggc gcacggcccc cggtggtgac ctgcgggttc tcgtggacgg
     1921 tgaagaacgg gcggtcgcgg ccgatggaac cttcgacggg cgcggcacgt tgaccctcgt
     1981 gcgcgccggt gagtggtgca cgcggctgga ccacccgctg ccgttcaacg agccgaggtt
     2041 ggcagagaaa gaatgagtac cgaagtgggc atcgacgtca cggtcgtgat gccggcgtac
     2101 aacgaggccg agatcctcga gcagtcggtc ggcgatgtgt gcaacggcct gcgcgaccgc
     2161 ggcgtctgct tcgagctgct cgtcgtcgag aacggctcga ccgacaacac gctcgcgctc
     2221 gcgcacgagc tcgccgagaa gtacgacgag gtgcgggccg agcattccga cgcgcccgac
     2281 tacggccgcg cgctgcgcca cggtctgctg ctcgcgcaag gcgccgcggt cgtgaacttc
     2341 gacgccgact actacgacct cgacttcctc caggccgcgg tcgccaaggt cacggccgag
     2401 ggtggacccg cgatcgtcgt cggctccaag cgcagtgagg gctcgaacga cacgcgcccg
     2461 ttcctgcgca agacggtcac gtggacgttc tccacgatcc tgcgcgtcgt gttcggcctt
     2521 aaggtctccg acacgcacgg catgaaggcg atgcggcgcg ttgccgtcgt tccctacgcg
     2581 cgtgcatgcc agttcggaca ggacctgttc gacaccgagt tgatcctgcg ctgcgaacgc
     2641 gcagggctcc cgacatccga gatcccggtc ccggtcatcg agctgcgacc cgcgcgtagc
     2701 tctatcctgc gccgcgtgcc tcgcacgatt ctcggattgt cgaagttgcg cgtcgcgctc
     2761 tggcgcgacc gcaaccgcgt tgtgtgacct gcctcacggc cgcgcgcatc gtcaccggtg
     2821 acggtggtgt ctcggcgtcg cccgcgtggg ttgtcgtcga agacggacgg atcgtcgcgt
     2881 ccggcagtgg cgccgcaccg caaccagccg aggatctcgg cgatgcgctc ctcgcccccg
     2941 gtttcgtcga tctgcaggtg aacggcacgg gcacgctcga cttcgcaacg gcgagcatcg
     3001 acgagctgcg cgcggcgatc gacagcttgg tcgccggtgg ttgcacgagc ctgctgccga
     3061 cgctgtgctc cgcgccgctc gacacgtatc ttcccgcgtt gcaacgcatc gccgacctgc
     3121 gggccacgtg cgacgcggtg ctcggcgtcc atctcgaagg cccgttcctc gggggcgcgc
     3181 caggggcgca cccgacggat ctggtgcggc cggtcgacct gccgtggctg gtcgggttgt
     3241 tggacgcgac cggcgacctc atccggctcg tcacactcgc gcccgaagcc gaccccggct
     3301 tcgcagcgac gcgagcactg actcaacgcg gcatcgccgt cgcgctcggc cactccatcg
     3361 ccggctatga cgaggcgcgc gccggtgccg acgcgggcgc gcgcatggtg acgcattgct
     3421 tcaacggcat gggaccgttg caccaccgtg cgcccgggct ccccggcgcc gcgctcgacg
     3481 accggcggct ggtgccgtcg gtgatctgcg atctcgtgca cgtgcacccc gcggtgctgc
     3541 ggatggtcac gaccgtgcgg cccgacgcgg tcgcggtcac cgatgccgtc ggcggcgacc
     3601 tcgtcgtggg agacggcggc gccgcgtacc tcgccgacgg cacgttggcc ggggcacgcg
     3661 tcacgatggc cgaggtcgtg cagaacctcg cgggcatcgg tctcgcgccg ggcgccgccg
     3721 tgcgcatggc gaccgggaac ggggcgaagg cgatcggtgc gaccgatcgg ggccgcatcg
     3781 cgccgggctg ccgcgccgac ttcgtcaccc tcgaccccga caccctcgcc gtccgcgagg
     3841 tctgggtggg cggcacacgc cgtatgtagg gtgcgcgggt ggcccgcgtc gcgttgttcg
     3901 tcacctgcgt cgcggaccag ctgtttcccg ataccgcgtg gtcgaccgtg cgcctgctcg
     3961 aagcggccgg cgacaccgtc gagttcccgg aggcgcagac ctgttgcggg caaccggcgc
     4021 tggccgccgg ggaacgcgac gccgtcccgc gcctcgctca tcacttcctc gacacctttg
     4081 ccggctacga cgcggtcgtc gcgccgtcgg gctcgtgcac ggcgatggtg caccactggt
     4141 acaaggagct cgtccccgag cgcgcggccg aggtcgaggc gctcgccgca cgcacgtatg
     4201 aattgacgca gtacctcgtc gacgtgcgcg ggctcgtcga cgtcggcgcg cgcgtcgacg
     4261 caaccgtcac ggtgcacgac gcctgccacg ggctgcgcac cctcggcctc gcgcacccga
     4321 cccgccgctt gttggcggcc gcgggcgcga ccatcgtcga actgacggag ccggagacct
     4381 gctgcgggtt cggcggcgtc ttcgccgccg agtaccccga ggtgtcgacc aagctcgccg
     4441 acgccaagtt ggccgacgcg cgcgccacga gcgcgcagta tctcgtgagc accgacctcg
     4501 cgtgcctcat gcatctcgaa ggccgggccc ggcgcgtgcg gtccgggccg cgcgcgatcc
     4561 acgtcgccga tctgctcgcg ccagggctcc cggcatgagc ggccacgggc tcgtcacgcc
     4621 gggcgagacg atgcgcgacc gtgctgcgct cgcggtgcag gacccgac
//
LOCUS       DASUGA010000636         7428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1590802, whole genome shotgun sequence.
ACCESSION   DASUGA010000636 DASUGA010000000
VERSION     DASUGA010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590802"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VFX21_12610"
     CDS             complement(<1..565)
                     /locus_tag="VFX21_12610"
                     /inference="COORDINATES: protein motif:HMM:NF017697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate hydrolase family protein"
                     /protein_id="HEX5096856.1"
                     /translation="MLDELLALPDVEERCVLRSRFGFLALHGGLEQHTAEIASSAAEL
                     SDASFYAVVQPDDLRWHVPSHRYAASASPELAAFLAHVDVVVSVHGYGGLRDSDDRWT
                     TALLGGTNRVLAADLAVALRTTLPEYTWIDDMTRIPSHLRGVHPQNPVNQPREGGVQL
                     ELPPRVRGYGHFWRDFDGPGFAPHTVRL"
     gene            complement(592..1308)
                     /locus_tag="VFX21_12615"
     CDS             complement(592..1308)
                     /locus_tag="VFX21_12615"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX5096857.1"
                     /translation="MRGVRSILVLAAMLLAVSCARANATQSSGRIAPPAPEVFLVGDS
                     ISDGAGQSIDATFERQGWHHYRVAIGGQSVADMQAYITSGAQLRKPDVMVIELGTNDM
                     GGVELDDANTGTVDAEARQRGYDDALDQLDTALRNMHDVLCVVWLDVADWSDLDFGPA
                     GHYNLQTWAPRYNAALRERQAHHPNLHVVSYAEQIRARGTAWMDDNFDEGWRIHPTTS
                     SGRQAVADIMVDGIHDSCGI"
     gene            complement(1521..2135)
                     /locus_tag="VFX21_12620"
     CDS             complement(1521..2135)
                     /locus_tag="VFX21_12620"
                     /inference="COORDINATES: protein motif:HMM:NF022169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HEX5096858.1"
                     /translation="MSATPGWYPDPSNPQIMRFWNGSEWTTERSVPVGIGEQPAARTP
                     ATTGTSTTGVQFQMPQLSTLAWLTFGGVALVALGAMLPWVEVHTAYGYSVRETPQGGS
                     AFLFWLLGAAIVALAWPARTGTLSKARCIGLTVIGSYLTLCAFTNIAAVGKLNDEHPG
                     AGISGGMGLWIYTLGTGLIVAGIVKAWLARRKEAAASATSTVLA"
     gene            2271..3383
                     /locus_tag="VFX21_12625"
     CDS             2271..3383
                     /locus_tag="VFX21_12625"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX5096859.1"
                     /translation="MHPVEVGVLGPLELRVAGRTVAITGPRQRSLVAMLALHANRVVP
                     VDGLVEAVWGDTPPDGADHTLQQHVSAVRKALAPDGRASAATSQLSTQSPGYVLAVAA
                     LDAEAFERSAATGFDAAANEQWAAAVAAFDGALACWRGAALADTRETPKLRAAAVRLD
                     EQRLGVIERRFDALLAAGQARAVVAELEHVVAEHPLREAFRAQLMLALYRSGRQADAL
                     AAYRAARDVLIDELGIEPGQALRSLEQAILEQRADLDVAPAVPADREIYATWRADLHA
                     SGGHVRLPDGQSVMLAEGVTLIGRDNDAQVRLVDNRVSRHHAQIESMLGRAVLRDLVS
                     TNGTAVNGEPIEERVLADGDVISVGGVELRFHRAPA"
     gene            3463..3822
                     /locus_tag="VFX21_12630"
     CDS             3463..3822
                     /locus_tag="VFX21_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096860.1"
                     /translation="MKQQRSAMAGIAAGVGALTLMLTACGGGGGGGGSASAFCKDYMS
                     LASISSTDPDVYDKVADKFEQLADEAPSDIKDELETMSEAANGTADGNLFAVDSDEAG
                     AAADRADAWVTEHCTDN"
     gene            complement(3819..7415)
                     /locus_tag="VFX21_12635"
     CDS             complement(3819..7415)
                     /locus_tag="VFX21_12635"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5096861.1"
                     /translation="MAGANPAFAQRFRREARLLARIDHPHVVTVFDFREHDDLHLLVM
                     ELLTGGTLAERKGRVSAETAIASVLAAASGLQHVHDGGILHRDVKPENLMFDRRGALK
                     VTDFGIARGDSMYATAVDITHAREFFGTPAYVSPEQAGHALGEGWSAVGVHSDQYSLA
                     AVLYELLTGRLTHDSSGGAIALCNRRMHDPARPLRDFAPSVPEEIEATVLRALARLPD
                     DRYESVEAFAVALGTAAAHALGPGWLERSEVSIREPGAILDAARGTGRGAAVRVEPPA
                     AIDVTVPLPARLRRSLDDVFAGRVEEQERWDDAWKLVRTGERGVWLVGGEAGVGKTSL
                     ISRLAARAHDDGAVVLYGRCDEDVGIPYQPWIEALSSLVTHAPRLLIEQHVAARGGEL
                     ARLIPELSRRTGVAVSVASDAEAERYALFGAVADLLTRATTIDRIVVVLEDLHWADKP
                     TVQLLRHLVQTAEPMRIMIVGTYRATDLGAAHPLTEAFAALRRERGVDFVDLRGLGDM
                     ELLALMEGIAGHELDADGLALRDAIAVETAGNAFFAHEILRHLVETGALVRQDGRWIA
                     TPDLAAHGLPVSVRQVVGERVARLGTDAQRLLRTASVIGREFDLRLVADAADVDEEVA
                     LDLLDAAVDAAVLHNVGPDRYTFAHALVEHTLYDELSPSRRGRMHRRIAEAIETRVGD
                     DTDDVVGELAFHWSAALVPDDAQKAIEYARRAGERALEQLAPDEGLRWFTKAQQLHGA
                     HGGDVHAYAQILVGLGAAQRQLGDETHRETLLAAANTARSIGDTDLLVAAVLANSRGW
                     ATLTGIVDEECVAAVEAALAAVGPDDSVERARLLAVLVAELTFDDDLVRRRAAADEAE
                     AIARRIGDDRALVAALIGLLSLPDRPGTQHLVWADEAIALAVALDDPVSLAIASASAV
                     TSATSFADRERFDRYLDLCTNASARVGQPMLLLRASGAQTLKAVLDGDLDGAEVLAND
                     ILAGAAGMSYAFVWYGSIIITIRGHQGRGAEVRQMIAMLAGDSQAGSISETARAGLLI
                     ADVQSHDFETARAGFAEQIEQGFSARDDQLWLTHVCINAHACARLEDREQAAPLLALL
                     EPYADLVAATPSLCMFSAPSCAGALAALLGRDEDADRYFARAIEITARLRAPFLLATA
                     QLEWARALTRRGLGATEQAATLVAAALAAAREHGYAEIERDALDLRAPGASH"
BASE COUNT         1120 a         2582 c         2637 g         1089 t
ORIGIN      
        1 tgagccgaac ggtgtgcggc gcgaaccccg gtccgtcgaa gtcacgccag aagtggccgt
       61 agccgcgcac gcgcggcggc agctcgagct gcactccccc ctcgcgcggt tggttgaccg
      121 gattctgggg atgcacgccg cggagatggc tcggaatgcg cgtcatgtcg tcgatccatg
      181 tgtactccgg cagcgtcgtt cgcaatgcga cagccaggtc ggccgcgagc acacggttgg
      241 tgccgccgag cagggcggtc gtccaccggt cgtcggagtc gcgtagaccg ccgtatccgt
      301 gcaccgacac gacgacgtcg acgtgcgcga ggaacgccgc gagctccggc gatgccgacg
      361 ccgcatagcg gtgcgacggc acatgccacc gaagatcatc cggctgcacg actgcataga
      421 acgacgcgtc gctcagttcg gccgcgctcg acgcgatctc ggccgtgtgc tgttcgagac
      481 caccgtgcag cgcgaggaat ccgaaacggg agcgcagcac gcaacgctcc tcgacgtcgg
      541 gcagcgccag cagctcgtcg agcacgaccc gattgtcgcg cgcaccggca cctagatgcc
      601 gcaggagtcg tggatgccat ccaccatgat gtcggcgacc gcctggcggc cggagctcgt
      661 cgtggggtgg atgcgccagc cctcgtcgaa gttgtcgtcc atccatgccg tgccgcgggc
      721 gcggatctgt tcggcatagc tcaccacatg caggttcgga tggtgcgcct gccgttcgcg
      781 tagcgccgcg ttgtaccgcg gcgcccacgt ctgcaggttg tagtgaccgg ccgggccgaa
      841 gtcgaggtcg ctccagtcgg cgacgtcgag ccagacgacg cacagcacgt cgtgcatgtt
      901 gcgcagtgcg gtgtcgagtt ggtcgagcgc atcgtcgtac ccgcgctgcc gtgcctcggc
      961 atcgacggtc ccggtgtttg cgtcgtcgag ctcgacgccg cccatgtcgt tggtgccgag
     1021 ctcgatgacc atcacgtcgg gcttgcgcag ttgcgcgccc gaggtgatgt acgcctgcat
     1081 gtcggccacc gactggccgc cgatcgcgac gcggtagtga tgccaaccct gacgttcgaa
     1141 cgtcgcgtcg atcgactgcc ccgcgccgtc gctgatgctg tcgccgacga ggaacacctc
     1201 gggcgcaggc ggcgcgatgc gacccgacga ctgcgtcgcg ttcgcgcggg cgcacgacac
     1261 cgcgagcagc attgccgcca acaccaggat ggagcgcacg ccccgcatgt cgtgttcctc
     1321 ggacgcctcg gcttgcggtt gagcaagccc atccatcgtg agcgttgccg caggtcagga
     1381 gcagccgcat ctgtgtcatt ggcgccgacg cgccggacca tttgtcacaa tctcgccgta
     1441 cgcgaaacag cccgccgagc agtgcgatcg cacgtccggc gggctgcgtt ctcggagatc
     1501 ggtgacgatg tttcccgggg ctaggcgagc acggtgctgg tcgccgacgc cgcggcttcc
     1561 ttgcgccggg cgagccacgc cttgacgatg ccggcgacga tgagaccggt gccgagcgtg
     1621 tagatccaca aacccatgcc cccactgata cccgcgcccg ggtgctcgtc gttgagcttg
     1681 cccacggccg cgatgttggt gaaggcgcac aacgtgaggt acgaaccaat caccgtcagg
     1741 ccgatgcagc gcgccttcga caacgtgccc gtgcgcgccg gccaggccag cgccacgatc
     1801 gccgcgccga gcaaccagaa cagaaatgcg cttccgccct gtggcgtttc ccgcacgctg
     1861 tagccgtagg cggtgtgcac ctcgacccac ggcaacatgg caccgagcgc gacgagcgca
     1921 acgccgccga acgtgagcca cgccaatgtc gagagctgcg gcatctggaa ctgcacgccc
     1981 gtggtgctcg tgccggtcgt ggccggcgtg cgcgcggcgg gttgctcgcc gatgccgacg
     2041 ggaacgctgc gttcggtcgt ccactcggaa ccgttccaga atcgcatgat ctgcgggttg
     2101 ctcgggtcgg ggtaccagcc gggggttgcg ctcatcgggt gctcctgttc gccacggccg
     2161 atcggccggt tgtgacgatc ctgacgacca ccgatcaggc gccactcagg ccgcgctcag
     2221 gcccacgacc cgcattgcac ggggcttcca ggccctggga gcatgcgccc gtgcatcctg
     2281 tcgaggtcgg tgtgctcggt ccgctcgagc tgcgagtggc gggccggacg gtggcgatca
     2341 cgggaccccg tcagcgcagc ctcgtcgcga tgctcgcgct ccatgcgaac cgggtcgtgc
     2401 ccgtcgacgg cctggtcgaa gcagtgtggg gcgacacgcc gcccgacggc gcggaccaca
     2461 cgctgcaaca gcacgtctcc gcggtccgca aagccctcgc gcccgacggc cgcgcgagcg
     2521 cggcgacgtc gcaactgtcg acacagagtc cgggctacgt gctcgcggtg gccgcgttgg
     2581 acgcggaggc gttcgaacgg tcggccgcga cgggattcga cgccgcggcg aacgagcagt
     2641 gggccgccgc cgtggccgcg ttcgacggcg cgctcgcgtg ctggcgcggt gccgcgttgg
     2701 cagacacacg cgagacgccg aagctgcgtg cggccgcggt tcggctcgac gagcaacggc
     2761 tgggcgtgat cgagcgccgc ttcgacgcgt tgctggcggc gggccaggcg cgcgccgtcg
     2821 tcgccgagct ggaacacgtg gtcgccgagc atccgctgcg cgaagccttt cgtgcgcagc
     2881 tcatgctggc gttgtaccgc agtggccggc aggccgacgc gctcgcggcc taccgcgcgg
     2941 cgcgggacgt gttgatcgac gagctcggga tcgaacccgg acaggcgctg cgttccctgg
     3001 agcaggcgat cctcgaacag cgcgccgacc tcgacgtcgc gccggccgtc ccggccgatc
     3061 gcgagatcta tgcgacatgg cgcgccgacc tgcacgcgtc gggcggccac gtccggctgc
     3121 ccgacgggca atccgtgatg ctcgccgaag gcgtcacgtt gatcggtcga gacaacgatg
     3181 cgcaggtccg gctggtcgac aatcgcgtca gtcgccatca cgcccagatc gagagcatgc
     3241 tcggacgcgc ggtgctgcgc gacctcgtgt cgaccaacgg caccgcggtg aacggcgaac
     3301 cgatcgaaga gcgcgtgctc gccgacggcg acgtcatcag tgtcggtggc gtcgagctcc
     3361 gcttccaccg cgcacccgcc tgagcgggcc ttgcgtcgcg cttgagcggc cctccgtacc
     3421 gtccgtcacg tcgagttcgg acggcgaaac acggagttgg acatgaagca gcaacgttcc
     3481 gcaatggcag ggatcgcggc cggcgtcggc gcgctgacct tgatgctcac cgcgtgcggc
     3541 ggtggtggcg gaggcggcgg cagtgcctcg gccttctgca aggactacat gagcctcgcc
     3601 agcatctcga gcacggatcc cgacgtctac gacaaggtcg cggacaagtt cgagcagctc
     3661 gccgacgagg ccccgagcga catcaaggac gagctcgaga cgatgtcgga ggccgcgaac
     3721 ggcaccgccg acggcaacct gttcgcggtg gacagcgacg aggcgggcgc cgcggccgac
     3781 cgcgccgacg cctgggtgac ggagcactgc acggacaact agtgcgaggc gccgggggcg
     3841 cggagatcga gcgcgtcgcg ttcgatctcc gcgtagccgt gctcccgcgc ggccgcgagc
     3901 gcggccgcga ccagcgtcgc cgcctgctcg gtggcgccca ggccgcgacg agtgagcgcc
     3961 cgggcccatt cgagctgcgc ggtggcgagc aggaaggggg ctcgtagccg cgccgtgatc
     4021 tcgatcgcgc gcgcgaagta gcggtcggcg tcctcgtcgc gaccgagcag ggcggccagc
     4081 gcgcctgcgc acgacggcgc cgagaacatg cacagcgacg gcgtcgcggc gacgaggtcg
     4141 gcgtacggtt cgagcagcgc gaggagcgga gccgcctgct cacgatcttc gaggcgggca
     4201 catgcgtgcg cgttgatgca cacgtgcgtc agccagagtt gatcatcgcg cgcggagaat
     4261 ccttgctcga tctgctccgc gaagccggcg cgagccgtct cgaagtcatg ggactgtacg
     4321 tcggcgatta acagtccggc acgcgccgtc tcggagatgc tcccggcctg actgtccccc
     4381 gcgagcatgg cgatcatctg ccggacctcg gcaccgcgac cctggtggcc ccggatcgtg
     4441 atgatgatgc tcccgtacca gacgaacgcg tagctcatgc ccgccgcgcc cgcgagaatg
     4501 tcgttggcga gcacttccgc gccgtccaag tcgccgtcga gcactgcctt gagcgtctgc
     4561 gcgccactcg cgcgcagcag cagcatcggc tggccgacgc gcgcggacgc gttcgtacac
     4621 aggtcgaggt aacgatcgaa acgttcgcgg tcggcgaacg acgtagccga cgtgaccgca
     4681 cttgcggacg cgatcgcgag cgatacgggg tcgtccagtg ccaccgcgag tgcgatcgcc
     4741 tcgtcggccc acacgaggtg ttgcgtgccc gggcggtcgg ggaggctcag caaaccgatg
     4801 agtgcggcca ccagcgcgcg gtcgtcgccg atccggcggg cgatcgcctc ggcttcgtcc
     4861 gcggccgccc gccggcgcac gaggtcgtcg tcgaacgtga gctcggcgac gagcacggcc
     4921 aacaggcgcg cccgctcgac actgtcatcg gggccgaccg ccgccagcgc ggcctcgact
     4981 gcggcgacgc actcctcgtc gacgatgccg gtcagcgtgg cccacccgcg actgttcgcc
     5041 aacaccgcgg cgacgagcag gtccgtgtcg ccgatgctgc gcgccgtgtt cgccgccgcg
     5101 agcaaggtct cccggtgcgt ctcgtcaccg agctggcgct gcgccgcgcc gagcccgacg
     5161 aggatctgcg cgtacgcgtg gacgtcgcca ccatgcgcgc cgtgcaactg ctgcgccttg
     5221 gtgaaccagc gcaaaccttc gtcgggcgcg agctgttcga gcgcgcgctc acccgcgcgt
     5281 cgcgcgtact cgatggcctt ctgcgcgtcg tcggggacga gcgcggccga ccagtggaac
     5341 gcgagctcgc cgaccacgtc gtccgtgtcg tcaccgacgc gcgtctcgat cgcctccgcg
     5401 atgcggcggt gcatgcgccc gcgccggctc gggctgagct cgtcgtagag cgtgtgctcg
     5461 acgagcgcgt gggcgaacgt gtagcgatcg ggcccgacgt tgtgcagcac ggcggcgtcg
     5521 accgccgcgt cgaggaggtc gagcgcgact tcctcgtcga cgtcggcggc atcggccacc
     5581 aggcgcaggt cgaactcgcg gccgatgacg gaggcagttc gcagcaagcg ctgcgcgtcg
     5641 gtgcccaggc gggcgacgcg ttcaccgacg acttggcgca cactcaccgg gagcccgtgc
     5701 gccgcgagat cgggtgtcgc gatccaacga ccgtcctgcc gcacgagcgc gcccgtttcc
     5761 accagatgcc gcaggatctc gtgcgcgaag aacgcgttgc cggccgtctc gaccgcgatc
     5821 gcgtcgcgca acgcgagacc gtcggcgtcg agctcgtgac cggcgatgcc ttccatgagc
     5881 gccagcaact ccatgtcgcc gagcccgcgc aggtcgacga agtcgacgcc gcgttcgcgc
     5941 cgcagcgccg cgaacgcctc ggtcagcggg tgggcggcgc cgaggtcggt ggcgcgatag
     6001 gtaccgacga tcatgatgcg catcggttcg gcggtctgga cgagatgccg cagcaactgg
     6061 acggtcggct tatcggccca gtggaggtct tcgagcacga cgacgatcct gtcgatcgtc
     6121 gtcgcgcggg tgagcaggtc ggcgaccgcg ccgaacagcg cgtaccgctc ggcctctgca
     6181 tccgacgcga ccgaaaccgc gacaccggtg cgccgggaca gctccgggat cagccgcgcc
     6241 agctcaccgc cacgcgcggc gacgtgttgt tcgatgagga gtcgtggtgc atgcgtgacc
     6301 agactcgaca gcgcttcgat ccacggctgg tacgggatgc cgacgtcttc gtcgcaacgt
     6361 ccgtacaaga ccaccgcgcc gtcatcgtgg gcgcgcgccg caagacgcga gatgagcgac
     6421 gtcttcccga cgccggcctc gccgccgacc agccacacgc cgcgctcacc ggtgcgcacg
     6481 agcttccacg cgtcgtccca ccgttcttgc tcctcgacgc ggcccgcgaa gacgtcgtcg
     6541 aggctgcgac gcaggcgcgc gggcagcggg acggtgacgt cgatcgccgc cggtggctcg
     6601 acccgtacgg cagcgccgcg accggtgccg cgcgcggcgt cgaggatcgc gcccggttca
     6661 cgaatgctca cctcggatcg ttcgagccag cccgggccga gcgcgtgcgc ggccgcggtc
     6721 ccgagcgcga cggcgaatgc ctccacggac tcgtagcgat cgtcgggcag gcgcgcgagc
     6781 gcacgcaaca cggtggcttc gatctcctcg ggaacggacg gcgcgaagtc gcgcagcggt
     6841 cgagccgggt cgtgcatccg gcggttacag agcgcaatcg cgccgccgct gctgtcgtgg
     6901 gtcagccggc cggtgagcaa ctcgtacagc acggcggcca ggctgtactg gtcgctgtgc
     6961 acaccgaccg cggaccagcc ctcgccgagg gcgtgcccgg cttgttcggg cgagacgtac
     7021 gcgggcgtgc cgaagaactc ccgagcatga gtgatgtcga ccgcggtcgc gtacatcgag
     7081 tcgccgcgcg cgatgccgaa gtcggtgacc ttgagcgcgc cgcgccggtc gaacatgagg
     7141 ttctcgggct tgacgtcgcg gtgcaggatc ccaccgtcgt gcacgtgctg gagtcccgag
     7201 gcggcggcaa gcacggacgc gatcgcggtt tcggccgaca cccttccctt gcgttccgcg
     7261 agggtgccgc cggtgaggag ctccatgacg agcaggtgca agtcgtcgtg ctcgcggaag
     7321 tcgaacacgg tcacgacatg cgggtggtcg atgcgcgcga gcagccgcgc ctcgcgccgg
     7381 aagcgctggg caaatgccgg atttgcgccg gccaactgtt tgatcgcg
//
LOCUS       DASUGA010000637         2014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1311501, whole genome shotgun sequence.
ACCESSION   DASUGA010000637 DASUGA010000000
VERSION     DASUGA010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311501"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VFX21_12640"
     CDS             complement(<1..346)
                     /locus_tag="VFX21_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096862.1"
                     /translation="MATDTERTIAPARERTRAGVRNNRRTRLLHTGTYLVTTALLYTG
                     WWLRTGHEGQPSVLSRWFDRPDVEVHRKAGWILVAIGAMAITIGIRGTFTFVRETVRV
                     NRGDGRWLVRWPL"
     gene            complement(330..821)
                     /locus_tag="VFX21_12645"
     CDS             complement(330..821)
                     /locus_tag="VFX21_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096863.1"
                     /translation="MNIDNAHPTSQRPRRTRRLTVLAILIAAFALLAAACGGDDDSTA
                     AGASGGAPTVAITAPTDGAQVGRSFDVHLAVNFPIGAPDTGREHVHLYYDGNRNEGEY
                     GIAYSDTFTVKGLSPGRHEIQAYVAHPDHSLTDAHSAPVIVDVGSAGTTDTMGSTDNG
                     NGY"
     gene            904..1461
                     /locus_tag="VFX21_12650"
     CDS             904..1461
                     /locus_tag="VFX21_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016433793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5096864.1"
                     /translation="MWDASDDALLAGYATGDRDAASVFVRRHQARMVGLALLITRDRV
                     EAEEVAQDAFVRAWRYAASYDPRRGPVSGWLLGIVRNVALDRVRVSSKRREYLLGEPA
                     FERLLDQQPDAADSVGTRDSAQRIVRAMQVLPIEQRDALLAATLHGFSAREISDAIDV
                     PLGTVKTRIRLGLRKLREQLGVQVA"
     gene            1458..>2014
                     /locus_tag="VFX21_12655"
     CDS             1458..>2014
                     /locus_tag="VFX21_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096865.1"
                     /translation="MKDTDEMTCAAFGDVAAELALGTLSGDERARALSHIETCVECRR
                     ETARLADTVEELLVLAPRVEPSPGLEHAVLARIEAANGGARAAGPGPRAGRRRRTAGR
                     VLALAAAVGLVVAALVVAVRPADHAPASRLADMRTESNQVVGSVVLTADPAAAVLDIP
                     HWNALVDSYGAAADAPYWLSLRARDG"
BASE COUNT          275 a          697 c          725 g          317 t
ORIGIN      
        1 ccagcggcca gcggacgagc caccgtccgt ctccgcggtt gacgcgaacg gtctccctca
       61 cgaacgtgaa cgtgccgcga atgccgatcg tgatcgccat cgcgccgatg gccacgagga
      121 tccaaccggc cttgcgatgc acctccacat cgggccggtc gaaccaccgg ctcagcacgc
      181 tcggctgacc ctcgtgcccg gtccgcaacc accagcccgt gtagagcaac gccgtcgtca
      241 ccagatacgt gccggtgtgc aggagtcgcg tccggcggtt gttgcggaca cccgcgcgcg
      301 tgcgctcgcg cgcgggtgcg atcgtccgtt cagtatccgt tgccattgtc ggtcgagccc
      361 atggtgtcgg tcgttccggc gctgccgacg tcgacgatga cgggcgcgga atgcgcgtcg
      421 gtcaagctgt gatccgggtg cgcgacgtac gcctggatct cgtgacgacc gggtgagagt
      481 cccttgacgg tgaaggtgtc gctgtaggcg atgccgtact cgccctcgtt ccggttgccg
      541 tcgtagtaca ggtgcacgtg ctcgcgcccg gtgtcgggcg caccgatcgg gaagttgacg
      601 gccagatgga cgtcgaacga ccggcccacc tgtgcgccgt cggtcggcgc ggtgatcgcg
      661 acggttggcg cgccgccgct cgcgccggcc gcggtgctgt cgtcgtcacc accacatgcc
      721 gccgcaagca gtgcgaacgc cgcgatgagg atcgcgagga cggtcagacg acgggtacgc
      781 cggggacgtt gggacgtggg atgtgcgttg tcgatgttca tgccgttctt accggccccg
      841 cgacggagcg ggatttctcc gtcccgagaa atccgcgcca cgccggactc aggtagtgat
      901 gacgtgtggg acgcctcgga cgacgcgctg ctcgccggct acgcgaccgg tgatcgtgac
      961 gccgcgtccg tcttcgtgcg ccggcaccag gcgcggatgg tcgggctcgc gctcttgatc
     1021 acgcgcgatc gcgtcgaggc cgaggaagtc gcacaggacg cgttcgtgcg ggcttggcgc
     1081 tacgcggcga gctacgaccc gcggcgcggc ccggtgtcgg gctggctgct cggcatcgtc
     1141 cgcaacgttg ccctcgaccg ggtgcgggtg tcgagcaagc gtcgcgagta tctcttgggc
     1201 gagcccgcgt tcgaacggtt gctcgaccag cagcccgacg ccgccgactc cgtcggcacg
     1261 cgcgacagtg cgcagcggat cgtgcgcgcc atgcaggtgc tcccgatcga gcaacgtgac
     1321 gcactgctgg cggcgacgct gcacggcttc agcgctcgcg agatcagtga tgcgatcgac
     1381 gtgccgctcg ggaccgtgaa gacgcgcatc cggctcggcc tccggaagct gcgcgagcaa
     1441 ctgggggtgc aggtcgcatg aaggacacgg acgagatgac ttgtgccgcg ttcggtgacg
     1501 ttgccgcgga gctcgccctc ggcacgctca gcggcgacga acgcgcgcgg gcgttgtcgc
     1561 acatcgagac ctgcgtcgaa tgtcggcggg agacggcgcg cctcgccgac acggtcgaag
     1621 agctgctcgt cctcgcgccc agggtcgaac cgtctccggg cctggaacac gcagtgctcg
     1681 ctcgcatcga ggcggccaac ggtggcgctc gcgcggccgg tcccggacct cgcgccggtc
     1741 gtcggcgccg gacggccggg cgcgtgctcg cactcgcggc cgcggtcggg ttggtcgtcg
     1801 ccgcgctcgt ggtcgcggtc cgccccgcgg atcacgcgcc ggcgtcacga ctggccgaca
     1861 tgcgcaccga atcgaaccag gtcgtcggct cggtcgtgct caccgccgac ccggccgccg
     1921 cggtgctcga catcccgcac tggaacgcgc tggtcgacag ctacggcgcc gccgcggacg
     1981 cgccgtactg gctgtcgctc cgtgcccgcg acgg
//
LOCUS       DASUGA010000638         2810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_514748, whole genome shotgun sequence.
ACCESSION   DASUGA010000638 DASUGA010000000
VERSION     DASUGA010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514748"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VFX21_12660"
     CDS             complement(<1..434)
                     /locus_tag="VFX21_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096866.1"
                     /translation="MARYRSFDAVRSVTLDLTVEPRLDDVRLRRAGLKAVMSGLEIAL
                     AAPVANRDDWLRNVRDALDAVREVWTRHIVETEAPGAFLDEVVSEAPRLSSPVSRLRR
                     EHNEILGVLHRAEQELAAGPGLDEYDEWSERKRAELTTLLVA"
     gene            complement(502..1491)
                     /locus_tag="VFX21_12665"
     CDS             complement(502..1491)
                     /locus_tag="VFX21_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5096867.1"
                     /translation="MAFLTMRQDFRAPDFSPASPQEIYAAALEQFAWADQQGFDMLVL
                     SEHHGLDDGWQPAPLTIAPAVLARSTNARVLISAAILPLHDPVRIAEQVAVIDNAFPG
                     RLWVVFGAGYREVEFEMAGMEHADRGKVLERYVQVVLDALSGEPLDFAGRTVRVTPKP
                     ITDPHQLFFVGGGVPAAARRAARLRLSMFPMNTDAAVRDAYFDEAKKTGFEGGFVLEP
                     VGPTFIHVADDPEKAWHDIAKFVMYEAQTYASFQTPGQHSTPGVRAESIDDLKASPQY
                     VVGTPDEVFEKLKDVPAMGGITFHPLAGGLPPKLAWESLELFAAKVLPRLRPS"
     gene            complement(1505..2458)
                     /locus_tag="VFX21_12670"
     CDS             complement(1505..2458)
                     /locus_tag="VFX21_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003968423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5096868.1"
                     /translation="MESTSHTVVSVVPEGKLAYGMQLPVQSLSVRVSQPWEQTGTVAD
                     MTKVAQAADNAGFLYVAVCHHVAIPPEPAEMMSTQWYDTVATLGYLAGQTTRTRLMSN
                     VFIAAYEHPLVAAKQFATLDVLSGGRIIFGIGAGHVEGEFDALGVSFAGRGKATDAAL
                     VQIKDALTNEWTGDVGQRPRPVQQPHPPIWIGGSGRPALRRVAHLGDGWIPQATTLDQ
                     LPLDIEWILRERDNVRPGAVPEIGYHLVAHVGDPTWELPKGVMKGSPEKIADFANSRL
                     GAIGVSHLQVRLLARDAQEQCDQIAAFGAEVGRHLTRKTLT"
     gene            complement(2497..>2810)
                     /locus_tag="VFX21_12675"
     CDS             complement(2497..>2810)
                     /locus_tag="VFX21_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5096869.1"
                     /translation="DMKVVQEEIFGPVVVALPFDEEDEGIALMNGTDFGLYDYVFTKD
                     TSRAYRVSKRLRAGNIGINTTQRNHNTPFGGTKYSGVGRDGGVFGLHAYTDLQSVVWP
                     G"
BASE COUNT          459 a          969 c          893 g          489 t
ORIGIN      
        1 aaggcgacca gcagcgtggt cagttcggct cgcttgcgct ccgaccattc gtcgtactcg
       61 tcgagcccgg gcccggccgc gagctcctgc tccgctcgat gaagcacacc caagatctcg
      121 ttgtgttcgc gccgcaggcg cgagaccggc gatgacagcc gcggtgcctc cgacacgacc
      181 tcgtcgagga acgcgcccgg cgcctcggtc tcgacgatgt ggcgcgtcca cacttcgcgc
      241 actgcatcca gcgcgtcgcg cacgtttcgc agccagtcgt cgcggttcgc gaccggcgcg
      301 gcgagcgcga tttccagtcc actcatcacc gccttcagcc cggcgcggcg caggcgcacg
      361 tcgtcgagcc tcggctcgac cgtgagatcg agcgtgacgc tgcgaacagc gtcgaacgag
      421 cggtaccggg ccattcgaac ctccgccgtt tgacgagtgg tccgaccgta cgcctttaca
      481 aacgttgtgc ctagaggtct tctagctcgg tcgcaggcgc ggcaggacct tggcggcgaa
      541 caactccagg ctctcccagg ccagcttcgg gggcagaccg ccggcgagcg ggtggaaggt
      601 gatgccgccc atggcgggaa catctttgag tttttcgaac acctcgtccg gcgtaccgac
      661 cacgtactgc ggtgacgctt tcaaatcgtc aatgctctcc gcgcgcacac ccggagtcga
      721 atgctggccc ggcgtctgga acgacgcgta cgtctgcgcc tcgtacatca cgaacttggc
      781 gatgtcgtgc cacgccttct cgggatcgtc ggcgacgtga atgaacgtcg gcccgaccgg
      841 ttcgagcacg aaaccgcctt cgaacccagt ctttttcgcc tcgtcgaagt acgcgtctcg
      901 caccgcggcg tcggtgttca tggggaacat cgacagccgc aagcgcgccg cccggcgggc
      961 cgccgccggc acgcccccgc cgacgaagaa caactggtgc gggtccgtga tcggcttcgg
     1021 cgtcacccgc acggtccggc cggcgaagtc gagcggttcg ccgctcagtg cgtcgagcac
     1081 gacctggacg tagcgctcga gcacttttcc gcggtcggcg tgctccatgc ccgccatctc
     1141 gaactcgacc tcgcggtagc cggcgccgaa aacaacccac aaccggcccg ggaacgcgtt
     1201 gtcgatcacg gcgacttgtt ccgcgatgcg aaccggatcg tgcaacggca ggatcgcggc
     1261 gctgatcaag acgcgtgcgt tcgtggagcg ggccagcacc gcgggcgcga tcgtcagcgg
     1321 cgccggctgc cagccgtcgt cgaggccgtg gtgctccgac agcacgagca tgtcgaagcc
     1381 ttgctggtcg gcccacgcga actgttcgag cgcagccgcg tagatctcct ggggggacgc
     1441 gggcgagaag tcgggcgccc ggaaatcctg gcgcatcgtg aggaacgcca tcaggccacg
     1501 tccgtcatgt aagtgtcttt cgagtgaggt gccgaccgac ctcggcgccg aaggccgcga
     1561 tctggtcgca ctgttcctgc gcgtcgcgcg ccagcagtcg cacttgcagg tgcgagacac
     1621 cgatcgcccc gagacgcgag ttcgcgaagt cggcaatctt ttcgggcgaa cctttcatca
     1681 cacccttcgg cagctcccac gtcggatcgc cgacgtgcgc gacgaggtgg tagccgatct
     1741 ccggcaccgc gcccggtcgc acgttgtctc gttcgcgcag gatccactcg atgtcgagcg
     1801 gcagttggtc gagggtcgtc gcctgcggga tccaaccgtc gccgaggtgc gcgacgcgcc
     1861 gcagcgcggg acgacccgag ccaccgatcc agatcggcgg gtgcggttgc tgcaccggcc
     1921 gcggccgctg gccgacgtcg ccggtccatt cgttcgtcag cgcgtccttg atctgcacga
     1981 gcgcggcgtc ggttgccttc cctcgccccg cgaacgacac acccagcgcg tcgaactcgc
     2041 cttccacatg gccggcgccg atgccgaaga tgatgcggcc gcccgagagc acgtcgaggg
     2101 ttgcgaactg tttcgccgcg accaacgggt gttcgtaagc cgcgatgaac acgttgctca
     2161 tcaaccgcgt ccgcgtcgtt tggccggcga gatatcccag ggtggcgacg gtgtcgtacc
     2221 actgcgtcga catcatctcc gccggctcag gcgggatcgc gacgtgatga cacactgcga
     2281 cgtacaagaa acccgcgttg tcggccgcct gcgcgacctt cgtcatgtcg gcaacggtgc
     2341 ccgtctgctc ccatggttgc gacacgcgca ctgacagcga ctgcaccgga agctgcatcc
     2401 cgtaggcgag ctttccttcg ggaacgacgg agacgacggt atgactggtc gactccacgc
     2461 tccgctcccc tccggtcgct gcgctgtcgt ctcttgctac cccggccata cgactgactg
     2521 cagatcggtg tacgcgtgca ggccgaacac accgccgtcg cgaccgacac cgctgtactt
     2581 cgtcccgccg aacggcgtgt tgtggttgcg ctgggtcgtg ttgatgccga tgtttccggc
     2641 ccgcaaacgc ttcgaaacgc gataggcgcg cgacgtgtcc ttcgtgaaca catagtcata
     2701 caagccgaag tcggtgccgt tcatcaacgc aatgccttcg tcttcttcgt cgaagggaag
     2761 cgcaacgaca accggaccga agatctcttc ctgtacgacc ttcatgtcga
//
LOCUS       DASUGA010000639         2072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_515863, whole genome shotgun sequence.
ACCESSION   DASUGA010000639 DASUGA010000000
VERSION     DASUGA010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515863"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VFX21_12680"
     CDS             <1..319
                     /locus_tag="VFX21_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-24"
                     /protein_id="HEX5096870.1"
                     /translation="DDTDWHEIALLYDRLARMVPSPVVELNRAVAIGMADGPEAGLAL
                     VDALARTDALRDYHLLPATRADLLRRAGRTAEAVAAYRDALERAGTDAERRYLERRLA
                     EIS"
     gene            362..850
                     /locus_tag="VFX21_12685"
     CDS             362..850
                     /locus_tag="VFX21_12685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5096871.1"
                     /translation="MTGEDDIRACVDAQYTRLVGVVSVVTGSHHLAEEAVQEAFARAW
                     ERAHKGYQFDHLAGWVATVALNLARSGRRRAASERAARARLNSVPPAAADPDVALVVR
                     DALVALPDRQRDAAVLYYLFDLDVATTARLLRVSDGTVKTALSRAREKLAPILRAAEL
                     EA"
     gene            847..>2072
                     /locus_tag="VFX21_12690"
     CDS             847..>2072
                     /locus_tag="VFX21_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096872.1"
                     /translation="MMPNDLDSELRDTLSTIVEEHAVSDVADPVAAIEQRVGRRHRNR
                     LLLVGSGIAAMIVFVVVVAAAISARDDGNKPRVVVSPSTTIPEPLLTNPVETMPKPPL
                     AVRGDAAVVWTGRDLVVWGGDVEAFNMGLPGKDRSYADGAVFDPRTRGWRAINPKTPL
                     PNTFDTPVGVATEDGVVFARGKHMARWIADDDVWIPYPDAAAPIQDLARSGDLLMSYS
                     ANAVFDLDAARWSSLPTPPLALERAATVSTGSELIVVGGPGTPFTDAAGLALDPAAKQ
                     WRKLAPFPRGMHAEALAADWDGRRVVVANYDMKAATYDPRADRWTTLPEVPARFSEWY
                     PTARSTGGRTAVFMAAAMVVLDGNRWTPLPAPTASLGTVATTRPGFGSQADNGVLFVF
                     GFQDDENVLTAIDLDA"
BASE COUNT          337 a          743 c          682 g          310 t
ORIGIN      
        1 ggacgacacc gactggcacg agattgccct cttgtacgac cggctcgcgc gcatggtgcc
       61 gtcgccggtc gtggagctga atcgagcggt cgcgatcggc atggctgacg gaccggaggc
      121 ggggctcgcc ctggtcgacg cgctcgcccg caccgacgcg ctccgcgact accacttgtt
      181 gccggcaacg cgagccgacc tgctgcgccg cgcgggccgc acggcggaag ccgtcgccgc
      241 ctaccgcgac gccctcgagc gggcgggcac cgatgccgaa cggcgctacc tggaacgccg
      301 cctcgcggaa atttcctgag acttgccaac cgaacgcacg tcctgaagcg tccttcatcg
      361 aatgaccggc gaagacgaca tccgggcctg cgtcgacgcg cagtacacgc gcctggtcgg
      421 cgtggtgagc gtcgtcaccg gatcgcacca tctggccgag gaagcagtgc aggaagcctt
      481 tgcacgcgcg tgggaacgag cccacaaggg ctaccagttc gaccacctcg ccggctgggt
      541 cgcgaccgtc gcactgaacc tggcccgtag cggccggcgc cgggcagcat ccgaacgcgc
      601 ggcgcgggcg cggctcaaca gcgttccgcc ggcggcggct gaccccgacg ttgcacttgt
      661 cgttcgcgac gcactggtcg cgctgccgga ccgtcagcgc gacgccgctg ttctctatta
      721 cctcttcgac ctcgacgtcg cgaccactgc gcgtctcttg cgggtcagcg acggcacggt
      781 gaagaccgcg ctctcacggg cgcgagaaaa gctcgcgccg atcctgcgcg ccgcggaact
      841 ggaggcatga tgccgaacga tctcgacagc gaactgcgcg acacgctctc aaccatcgtc
      901 gaggaacacg cggtttccga cgtcgccgat cccgtcgcgg cgatcgaaca gcgcgtcggc
      961 cggcgccacc ggaaccggct gctcctcgtc ggcagtggca tcgccgcgat gatcgtgttc
     1021 gtcgtcgtag ttgccgccgc aatttcggcg cgcgacgacg gcaacaagcc gcgcgtcgtc
     1081 gtctcgccga gcaccacgat tcccgaaccg ttgctcacga atccggtgga gacgatgccg
     1141 aagccgccgc tcgcggtgcg cggcgacgcc gcggtcgtgt ggaccggacg cgatctggtc
     1201 gtgtggggcg gcgacgtcga agcattcaac atgggcttgc ccggcaaaga ccgcagctac
     1261 gccgacggtg ccgtcttcga tccacgaaca cgcggctggc gggccatcaa tccgaagaca
     1321 ccgctgccga acaccttcga cactccggtc ggcgtcgcga ccgaagacgg tgtcgtgttc
     1381 gcacgcggca agcacatggc gcgctggatt gcggacgacg acgtgtggat tccctatccc
     1441 gacgcggcgg caccaattca ggacctcgcc cgatcgggcg acctcctcat gagctacagc
     1501 gcgaacgcgg tattcgatct cgacgctgcg cggtggtcat cacttcctac gccgccattg
     1561 gcgttggaac gcgccgcaac ggtttcgacc gggtcggaac tgatcgtcgt cggtggaccg
     1621 ggcacgccgt tcaccgacgc cgcgggcctc gcgctcgacc ccgcggcgaa gcagtggcgc
     1681 aagctcgcgc cctttccccg aggcatgcat gccgaagcac tcgccgccga ctgggacggc
     1741 aggcgcgtgg tcgtcgccaa ctacgacatg aaagccgcaa cctacgaccc tcgcgccgac
     1801 cgctggacga cgttgcccga agtcccggcg cgcttctccg agtggtaccc gacggcacgc
     1861 tcgaccggcg gccggacggc cgtgttcatg gcggcggcga tggtggtcct cgacggcaac
     1921 cgctggactc ccctgccggc gccgacggcc tcgttgggaa ccgttgcgac gacccggccc
     1981 ggattcggat cgcaggccga caacggcgtg ctcttcgtct tcggcttcca ggacgacgaa
     2041 aacgtgctca ccgccatcga cctcgacgca at
//
LOCUS       DASUGA010000640         2675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_860911, whole genome shotgun sequence.
ACCESSION   DASUGA010000640 DASUGA010000000
VERSION     DASUGA010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860911"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VFX21_12695"
     CDS             <1..797
                     /locus_tag="VFX21_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096873.1"
                     /translation="ANTEGRARVLVGDATQRAQAALASGARATPEAIAQAEQDVADAQ
                     TKLTKTQGALSTADGAITDWRADRGPEDPSAQIAQIDRRLAELRDQLAAAVSAQQDTT
                     TLDGQIAQAQQDHFDAESAQKQLAGLEDRRAAAATEVEQATAAVEAAQARLTRYQAGT
                     TGSISVAPINTETVVAARGFGAGVTIVTLVALAATLGVLALATRPRPELALDLTAPQP
                     ADPEPAPEPEPEPEPSPAAAAQRVDGLPPRPTRPFTTVKLRSPREH"
     gene            1017..1727
                     /locus_tag="VFX21_12700"
     CDS             1017..1727
                     /locus_tag="VFX21_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096874.1"
                     /translation="MSRNPRFRRRHVLTSLALVTALGLGIAACGGDDDDSSAASSPSS
                     ERVTQPISESTPSASTKAADLRATLTNLLEEHVYLAGIATGTALAGNDLQPAADTLDE
                     NSVALSEAIGSVYGDQAGSTFLGLWRSHIQFFVDYTNAAATGDDAGKMKARDDLDGYA
                     HDFAAFLTGANPKFDAATIEDALKHHAGTLFDAIDAQAAKDPSQYTKLRIAAQHMPDV
                     AAVLADGIVQQFPEKFTD"
     gene            1826..2515
                     /locus_tag="VFX21_12705"
     CDS             1826..2515
                     /locus_tag="VFX21_12705"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEX5096875.1"
                     /translation="MAGSLLFVTQVAPYRDGPAGVHGVLGQAAVGVAEIAELHGLTPH
                     VVADVRDLAPAALEQAAALALFTIGETPWSAPQRAAVLVRVRQGALAVCAIHSATDSC
                     YGWPEYAALVGARFDGHPWTQSFVADVCDVQHPSCAHLGDAWHWHDEVYQFRDLRPDA
                     RVLLRVRDGELDLDATGARPPSFGYPLAWCFEEGGGRVFSTSLGHFPAAWESPAYLRH
                     LAGGLGWALRS"
BASE COUNT          429 a          992 c          898 g          356 t
ORIGIN      
        1 acgcaaatac cgaaggtcgc gcgcgtgtgc tggtcggtga cgcgacacag cgcgcccaag
       61 cggcacttgc gtcgggcgcg cgcgcgaccc cggaggccat cgcgcaggcc gagcaggatg
      121 tcgcggacgc gcagacgaaa ctgaccaaga cgcagggcgc actgagcacg gccgatggtg
      181 cgatcaccga ctggcgagcg gaccggggac ccgaggatcc gtccgcgcag atcgcacaga
      241 tcgaccggcg gctcgccgag ctgcgcgacc aactcgccgc ggccgtgagc gcgcagcagg
      301 acacgacaac gctcgacggc cagatcgcgc aagcgcaaca ggaccacttc gacgccgagt
      361 ccgcacaaaa gcaactggcc ggtctcgagg atcgccgcgc cgcggccgcc accgaagtgg
      421 agcaggcgac ggccgcggtc gaagccgcgc aagcgcgtct cacgcgctac caggccggaa
      481 cgaccggctc catctcggtg gcgcccatca acacggagac cgtcgtcgcg gcgcgagggt
      541 tcggcgccgg cgtcacgatc gtcacgctcg tcgcgctggc cgcgacgttg ggcgtccttg
      601 cgttggcgac gcgtccccgc cccgagctcg cgctcgacct caccgcgccg cagccggccg
      661 atccagagcc ggcaccggaa cccgaacccg agcccgagcc gtcaccggcg gcggccgccc
      721 aacgggtgga cggcctgcca ccgcgtccga cgcgcccgtt caccacggtc aagctgcgtt
      781 ctccacgcga gcactgacgg gggaggcctc cgactccaaa ccgacggtgg cctcagtgtc
      841 ctccctacgc gcgaagcgcc cccgaggacc gtctcggcca ctcgcggagt ctttcggcgg
      901 gccaacaatc cgggctgcct cacgcgagca cgtcgaaagc gacaaggaaa cgcgtcatct
      961 cctcccggga gcggcaccga agaaccgccg ttccgacctt gaaggagatc gcaatcatgt
     1021 ctcgcaatcc ccgtttccgg cgccggcacg tcctgacgtc gctcgcactc gtcaccgcgc
     1081 tcggccttgg catcgccgcc tgcggtggcg acgacgacga cagcagtgcg gcatcgtcgc
     1141 cgagcagcga acgagtgacc caaccgatca gtgagtcgac gccgagcgcg tcgacgaagg
     1201 ccgcggacct ccgggccacg ctcaccaact tgctcgagga gcacgtgtac ctcgcgggca
     1261 tcgcgaccgg cactgcactc gcgggcaacg acctgcaacc cgcggccgac acactcgacg
     1321 agaactccgt tgcgttgtcc gaagcgatcg ggtccgtcta cggcgaccag gccggcagca
     1381 cgttcctcgg tttgtggcgc agccacatcc agttcttcgt ggactacacg aacgcggccg
     1441 cgacgggcga cgacgccggc aagatgaagg cccgcgacga cctggacggc tacgcacacg
     1501 acttcgcggc gttcctcacc ggtgcgaacc cgaagttcga cgccgcgacg atcgaggacg
     1561 cgctgaagca ccacgccggc acgttgttcg acgcgatcga cgctcaggcc gcgaaggacc
     1621 cgtcgcagta cacgaagctg cggatagcgg cccagcacat gccggatgtt gccgcggtcc
     1681 tggcggacgg catcgtccag cagttccccg agaagttcac ggactgagga accctgtccg
     1741 agggctccgg gagtgcaggc gccgggtggc atcgccatcc ggcgtctcgc gttcgggacc
     1801 cgaccgggga ctaggtttcg cgctcgtggc cggttccctg ctgttcgtga cgcaggtcgc
     1861 gccctaccgc gacggtcccg cgggcgtcca cggggtcctc gggcaggcgg ccgtcggggt
     1921 ggccgagatc gcggaactgc acgggctcac gccccatgtc gtcgccgacg tccgcgacct
     1981 cgcgcccgcc gccctcgagc aggccgcggc gttggcgctg ttcaccatcg gcgagacccc
     2041 gtggtccgcg ccccagcgcg ccgccgtcct cgtgcgggtc cggcaaggcg ctctcgccgt
     2101 gtgcgccatc cactcggcca ccgactcctg ctacggctgg cccgaatacg ccgcgctcgt
     2161 cggcgcccgc ttcgacggcc atccgtggac acagtccttc gtcgcggacg tgtgcgacgt
     2221 gcagcacccg tcgtgtgcac atctcggcga cgcctggcac tggcacgacg aggtctacca
     2281 gttccgcgac ctgcggcccg acgcgcgcgt gctgctgcgc gtgcgcgacg gcgagctcga
     2341 cctcgacgcg accggtgcgc gaccaccgtc gttcggctat ccgctcgcgt ggtgtttcga
     2401 ggaaggcggc gggcgagtgt tctcgacgag cctcggccac tttcccgcgg catgggaatc
     2461 gccggcgtac ctccgtcacc ttgccggcgg gctcgggtgg gcgctgcgat cgtgacgcgc
     2521 acgcgcaact tctccgactg gtcctactgg cggctgcacc tcgagacgat caccgagaac
     2581 gacccgctgc cgggcgacat gcaggagctc gaagcgtggc gcgcgagcac acgcgcacgc
     2641 gtgctcgacc tgctcggtcc cgcgccgaag aaggt
//
LOCUS       DASUGA010000641         2302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_201812, whole genome shotgun sequence.
ACCESSION   DASUGA010000641 DASUGA010000000
VERSION     DASUGA010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201812"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2154
                     /locus_tag="VFX21_12710"
     CDS             <1..2154
                     /locus_tag="VFX21_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5096876.1"
                     /translation="ASDALAVPGVRAVFTAADLNPLAREQWYTMLGPTMPTTPLPPLA
                     DGEARFVGDAVALVIAETRALAEDATELVVVDYEPLPPVVDYLVAEDAEGTVHGDYPN
                     NVIGQLAGMPASSLDELFDGAAHVIEETIAQQAYAAVPMETRGMVVEWDPSAGELTIW
                     AATQAPHEVRAYCSRLLGLPEHRIRVIMRDTGGAFGQKIMVNREEMCLMLAAAKVPAA
                     VKWIEDRRENLLAAGKSRQEQGDVRMAFDADGTMVAAHIDYVQDSGAYPVPWPVGTGT
                     LAAMLLPGPYKLSKATFTSKFFFTNTAGRTAYRGPWQFESVAREVMLDIAARRIGIDP
                     IELRRRNLLSADDMPFTAPCGMTFDRVTPLETFDHAIEILDYDAFRREQEAARAEGRY
                     LGVGTSTYVEPTTSGTGAFGSEGATIRIEPSGTVNVYVAGGSAGNSIETSAVQLAADA
                     LGVRMEDVRAIQGDTALTPYGTGTGGSRSGGMLAGAIGEACAILRDRLIALAAHKLEA
                     APEDIELAEGRAFVRGTPSAGMTITELAEFTYKNPMGVPPEIEAGLEASARFTAKSAS
                     LWANATHVCTCEVDVVTGRVTLLRYIVAEDCGPMINPNVVEGQIAGGTVQGIGGVLLE
                     HLAYDEDGNPVTTTFMDYLLPTAGEVPLIEYGHVETPSRGPGGYKGVGEGGCIGAPAA
                     VVNAVADALAPLGVVVNRLPLGPSQILALLEDGKR"
BASE COUNT          355 a          776 c          848 g          323 t
ORIGIN      
        1 gcgtccgacg cgcttgcggt gccgggcgtg cgcgccgtgt tcaccgctgc cgacctcaac
       61 ccgctcgcgc gcgagcagtg gtacacgatg ctcggcccca cgatgccgac cacaccgctc
      121 ccgccgttgg ccgacggcga ggcccgattc gtcggtgacg cggtcgccct cgtcatcgcc
      181 gagacgcgcg cgttggccga ggacgcgacg gagctcgtcg tcgtcgacta cgagccgttg
      241 ccgcccgtcg tcgactacct cgtggccgag gacgccgagg gcacggtgca cggcgactac
      301 ccgaacaacg tcatcgggca gctcgcgggc atgccggcgt cgtcactcga tgagctgttc
      361 gacggcgcgg cccacgtgat cgaggagacg atcgcgcagc aggcgtacgc cgcggtgccg
      421 atggagacgc gcggcatggt cgtcgaatgg gacccgtcgg cgggtgagct cacgatctgg
      481 gcggccacgc aggcaccgca cgaggtgcgg gcgtactgct cgcggctcct cggtctgccg
      541 gagcaccgga tccgcgtgat catgcgcgac accgggggtg cgttcgggca gaagatcatg
      601 gtgaaccgcg aggagatgtg cctgatgctc gcggccgcca aggtgccggc cgcggtgaag
      661 tggatcgagg accggcgcga gaacctgctc gcggccggga agtcgcgaca ggagcaaggc
      721 gacgtgcgca tggcgttcga cgccgacgga acgatggtcg ccgcgcacat cgactacgtg
      781 caggactccg gcgcgtaccc ggtgccgtgg ccggtgggta cgggaacgtt ggccgcgatg
      841 ctcctgcccg gtccgtacaa gctgtcgaag gcgacgttca cgtcgaagtt cttcttcacc
      901 aacacggcgg gccgcaccgc gtaccgcggg ccgtggcagt tcgagtcggt ggcgcgcgaa
      961 gtgatgctcg acatcgcggc gcggcgcatc gggatcgatc cgatcgagct ccgtcgtcgc
     1021 aatctgctct cggccgacga catgcccttc accgcgccgt gtggcatgac gttcgaccgg
     1081 gtcacgccgc tcgagacgtt cgaccacgcg atcgagatcc tcgactacga cgcgtttcgt
     1141 cgtgagcagg aagcggcccg cgccgagggg cgctacctcg gagtcggcac cagcacgtac
     1201 gtcgagccga ccacgagcgg cacgggcgcg ttcggcagtg agggcgcgac gatccgcatc
     1261 gagccctcgg gcacggtcaa cgtgtacgtg gccggtgggt ccgcgggcaa cagcatcgag
     1321 acctcggcgg tgcagctggc cgccgatgcg ctcggcgtgc gcatggagga cgtgcgcgcc
     1381 atccagggcg acactgcgct cacgccctac ggcaccggca ctggcggcag ccgcagcggc
     1441 ggcatgctcg ccggcgcgat cggcgaggcg tgcgcgatcc tgcgcgaccg tctgatcgcg
     1501 ctcgcggcgc acaagctcga agcggcgccc gaggacatcg agctggcgga gggacgggcc
     1561 ttcgtccgcg gcacgccctc ggcggggatg acgatcacgg agctcgccga gttcacgtac
     1621 aagaacccga tgggcgtccc gccggagatc gaggccgggc tcgaggcgag tgcgcggttc
     1681 accgcgaagt cggcgtcgtt gtgggccaac gcgacgcacg tctgcacgtg cgaggtcgac
     1741 gtcgtgaccg gccgcgtcac gctgctgcgc tacatcgtcg ccgaggactg cggcccgatg
     1801 atcaacccca acgtcgtcga gggacagatc gccggcggca ccgtccaggg gatcggcggc
     1861 gtcctcctcg agcacctcgc ctacgacgag gacggcaacc ctgtcacgac gaccttcatg
     1921 gactacttgc tgcccacggc gggggaggtg cccctcatcg agtacgggca cgtcgagaca
     1981 cccagccggg gcccgggcgg atacaagggc gtgggtgaag gcgggtgcat cggcgcaccg
     2041 gcggcggtcg tgaacgcggt ggccgacgcc ctcgcgccgc tcggcgtcgt cgtgaaccgg
     2101 cttccgctcg gaccgtcaca gatcctggcg ctgctcgagg acgggaagcg ctgatcgacc
     2161 gatgggctga tcccacgtca gccttgccgc gcgttggggg gacgcgatga ctgccgacga
     2221 gccgtccgac cggagtgagg cgctggtcgt cgaatggatc gagaaggaga tcgggccggt
     2281 gcgcgccatc gcgcgccagg gt
//
LOCUS       DASUGA010000642         4127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1903551, whole genome shotgun sequence.
ACCESSION   DASUGA010000642 DASUGA010000000
VERSION     DASUGA010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903551"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VFX21_12715"
     CDS             <1..184
                     /locus_tag="VFX21_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096877.1"
                     /translation="ISNETMALAGPDGSPKPADAAKYVLTHPLEIPSLVRLARQSAKA
                     TANAAIAAIAACERAV"
     gene            complement(181..1443)
                     /locus_tag="VFX21_12720"
     CDS             complement(181..1443)
                     /locus_tag="VFX21_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5096878.1"
                     /translation="MPRRCDNYARRIALADIDFTDLDNFANGFPHALFEIHRREAPVS
                     WHEPTAHTPDGEGFWSVATYAETLEVQRDPVTYSSERGGTRKHGGTLLQDLPVAGLVL
                     NMMDDPRHARIRRLVSMGLTPRTVRDLEGELRRRTRALVDAIEPGRPFDFLVDVAAEP
                     PMQMICMLLGVPEHDRHALFEAVEHIFDFRGDREAFETTHEGEAAQHHMAVYGTALIA
                     EKRAHPTDDMLSVVVHATLDDVDPPSLTDSELFLFFSLLFSAGSETTRNAIAGGLLAL
                     MEQPDQLGALRNDRALLSGAIEEMLRWTTPSPSKRRTATAPTTLGGHAIEPGDKVLFW
                     EGSANRDERVFADSMQFDIRRDPNPHLAFGHGVHFCLGASLARREMQVVFEELLDRYA
                     TFELVEPVEWSRSNRHTGIRHMHIAATR"
     gene            1453..1962
                     /locus_tag="VFX21_12725"
     CDS             1453..1962
                     /locus_tag="VFX21_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096879.1"
                     /translation="MDAATAALIGTAIGGLSSLAVAALGTRQARRARAEERRHDLDTE
                     RRATYIRFLTHSRDVRYAALRAYQQRAFRSVGEVDALLTQLSSAFYMIALMAPDEIVA
                     DAQALREAVFDLWRLARDEPNADPDVWRAQLERVWAAQRGFRQLVRRELHLTRHSGTP
                     PRECARLPP"
     gene            1959..3077
                     /locus_tag="VFX21_12730"
     CDS             1959..3077
                     /locus_tag="VFX21_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007229632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEX5096880.1"
                     /translation="MTGPDTEARRAFQELIALLQEIDERYLGDEWNANGYGDLADGFR
                     SVANTIEGAFELMFDLDPQRPFFRRIVTRTRKMLGDNPDAIYYTAPVRDDLVYRVTGN
                     LAGAVYLSFTVELNTADGGYSTQTAGVLNQRDFDVDANGNFALVFGGPERERNWLALP
                     PRAGEIIVRCYFEEPDPIATDPNRGVPLYIEPEQPLPPPAPWSDASVAAGLRRVSAFV
                     RNRTLEQPKTGEREQPSWVSTVPNMFPPPETPGDFAFAAFDAAYTMAPYLLGPDEALV
                     ITGRWPKCDFGNVSLWNRYLQTYDYAHRPVSRNRANTTLEPDGSFRMVIAHDDPGVPN
                     WIDTEGRGFGMVFWRFFLPEGEIETPQATVVKLADVRG"
     gene            3070..>4127
                     /locus_tag="VFX21_12735"
     CDS             3070..>4127
                     /locus_tag="VFX21_12735"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5096881.1"
                     /translation="MADLNREKFADAACMATGLSDFGEASWQEGLDRLLDSLENEADL
                     NDLGRTIVEGEVVGYLTNRLEILSYRKEHPEIAEADVVPPIVIVGQGRTGTTILHDLL
                     AQDPATRVPLTWEVDAPVPPPETAMYHTDPRVAASDESLAMVDLVIPGFRSMHPMGAQ
                     LPQECVRITALDFKSMIHPTQYYVPSYARWLLNEADMSSAYRWHRIFLQHLQSKHPTD
                     RWILKSPGHIWCLEALLAEYPDALLVQTHRDPLRIIASLGSLVSTLRSLASSNTSIPV
                     AAGEFADYIIDGLDRSVDARENGIVSDDRVVDVYFDQFMADPFAVIAKIYAAAGFEYT
                     ADAEARMRAHLGDNPQDK"
BASE COUNT          662 a         1416 c         1387 g          662 t
ORIGIN      
        1 gatcagcaac gagacgatgg cgcttgccgg ccccgacggc tcgccgaaac cggcggacgc
       61 ggcgaagtat gtgctgacgc atccgctcga gatcccgagc ctggtgcgcc ttgctcgcca
      121 gtcggcgaag gcgacggcga acgcagccat cgccgcgatc gcggcctgcg aacgcgcggt
      181 ctagcgcgtc gcagcgatat gcatgtggcg gatgccggtg tggcggttgc tgcgcgacca
      241 ctcgaccggc tcgaccagtt cgaacgtcgc atagcggtcg agcagttctt cgaacaccac
      301 ttgcatctcg cgccgagcga ggctcgcgcc gaggcagaag tgcacgccgt gaccgaacgc
      361 gaggtgcggg ttcgggtcgc ggcggatgtc gaactgcatc gagtcggcga agacgcgctc
      421 gtcgcggttc gcggagccct cccagaacaa caccttgtca cccggctcga tcgcgtgccc
      481 gccgagcgtc gtcggtgcgg ttgccgtgcg gcgcttcgac ggtgacggcg tcgtccaccg
      541 cagcatctcc tcgatcgcgc cggagagcaa cgcgcggtcg ttgcgcagtg cgccgagctg
      601 gtcgggctgc tccatcagtg cgagcaggcc gcccgcgatc gcgttgcgcg tcgtctcgga
      661 gccggccgag aacagcaacg agaagaagag gaacagctcg ctgtcggtca ggctcggcgg
      721 gtcgacgtcg tcgagcgtcg cgtgcacgac gacggagagc atgtcgtcgg tcgggtgcgc
      781 tcgtttctcg gcgatgagcg cggtgccgta cacggccatg tgatgctgcg cggcttcacc
      841 ttcgtgcgtg gtctcgaatg cctcgcggtc accgcggaag tcgaagatgt gctcgaccgc
      901 ctcgaagagt gcgtgacggt cgtgctcggg cacgccgagc agcatgcaga tcatctgcat
      961 cggtggctcg gcggcgacgt cgacgaggaa gtcgaatggc cggcccggct cgatcgcgtc
     1021 gacgagcgca cgcgtccggc gccgcagctc gccttcgagg tcgcgcaccg tgcggggcgt
     1081 gagtcccatg ctgacgaggc ggcggatacg cgcgtggcgc gggtcgtcca tcatgttcaa
     1141 cacgaggccg gccacgggca ggtcttggag cagcgtgcca ccgtgcttgc gcgtgccgcc
     1201 gcgctccgac gagtacgtca ccggatcgcg ctgcacctcg agtgtctcgg cgtacgtcgc
     1261 gaccgaccag aagccttcgc cgtcgggtgt gtgcgcggtt ggttcgtgcc aggagaccgg
     1321 cgcctcgcgg cggtggatct cgaacagcgc gtgcgggaac ccgttcgcga agttgtccaa
     1381 atcggtgaag tcgatgtcag cgagcgcgat gcgccgagcg tagttgtcac accggcgcgg
     1441 cacgctgtgg tcatggatgc cgcgaccgca gcactgatcg gaaccgccat cggcggactg
     1501 tcgtcgttgg ccgtcgccgc gctcgggacc cggcaggcgc ggcgcgcccg cgccgaggag
     1561 cgccggcacg acctcgacac cgaacggcgc gccacctaca tccgtttcct gacgcattca
     1621 cgagacgtgc gctacgccgc gctccgggca tatcaacagc gcgcgttccg ctcggtcggt
     1681 gaggtcgacg ccttgctgac gcaactgtcg tcggcgttct acatgattgc cctgatggcg
     1741 cccgacgaga tcgtggccga cgcgcaggcg ttgcgcgagg cggtcttcga cctctggcgg
     1801 ctggcccgcg acgagcccaa cgcggatccc gacgtgtggc gcgcgcagct cgagcgagtg
     1861 tgggcggcgc agcgagggtt ccggcagctc gtgcgccgcg agctgcacct cacacgccac
     1921 tcgggcacac caccgcgcga atgcgcgagg ctgccgccat gaccggtccc gacaccgaag
     1981 ctcggcgggc gttccaggag ctcatcgcac tgctccagga gatcgacgag cgctacctcg
     2041 gcgacgagtg gaacgccaac gggtatggcg acctggccga cggcttccgc agcgtcgcca
     2101 acacgatcga gggtgcgttc gagctgatgt tcgacctcga tccgcagcgt ccgttcttcc
     2161 ggcggatcgt gacccgcacg cgcaagatgc tcggcgacaa ccccgacgcg atctactaca
     2221 ccgcgccggt acgcgacgac ctcgtgtacc gcgtgaccgg caacctcgcc ggcgcggtct
     2281 acctgtcgtt cacggtcgag ttgaacactg ccgacggcgg ctacagcacg cagactgcgg
     2341 gcgtgctcaa ccagcgcgat ttcgacgtcg atgcgaacgg caacttcgcg ttggtgttcg
     2401 gcggtcccga acgcgagcgg aactggctcg cgttgccgcc gcgtgccggt gagatcatcg
     2461 tgcgctgcta cttcgaggag cccgacccga tcgcgaccga ccccaaccgc ggcgtgccgc
     2521 tgtacatcga gcccgagcag ccgctgccac cgcccgcacc gtggagcgac gcgtcagtgg
     2581 cggcgggatt gcggcgcgtc agcgcgttcg tgcgcaatcg cacgttggaa cagcccaaga
     2641 cgggggagcg cgagcagccg tcgtgggtgt cgacggtccc gaacatgttc ccgccgcccg
     2701 agacgcccgg cgacttcgcg ttcgccgcgt tcgacgccgc gtacacgatg gcgccctatc
     2761 tgctcggtcc cgacgaagcg ctcgtgatca ccggccgctg gcccaaatgc gacttcggca
     2821 acgtgtcgtt gtggaatcgc tacctgcaga cctacgacta cgcgcaccgg ccggtgtcgc
     2881 gcaatcgcgc caacaccacg ctcgagcccg acggctcgtt ccgtatggtc atcgcacacg
     2941 acgatcccgg cgttcccaac tggatcgaca ccgagggccg cgggttcggc atggtgttct
     3001 ggcgcttctt cctgccggag ggcgagatcg agaccccgca ggcgacggtc gtgaaactgg
     3061 cggacgtgcg tggctgatct caaccgcgag aagttcgccg atgccgcgtg catggcgacc
     3121 ggcctgagcg acttcggcga ggcgagttgg caagagggcc tcgaccgctt gctcgactcg
     3181 ctcgagaacg aagccgatct caacgacctc ggccgcacga tcgtcgaagg tgaagtcgtc
     3241 ggctacttga ccaaccggct ggagatcctc tcgtatcgca aggagcatcc ggagatcgcc
     3301 gaggccgacg tcgtgccgcc gatcgtcatc gtcggccagg gccgcaccgg caccacgatc
     3361 ctgcacgacc tcctcgcgca agatcccgcg acgcgcgtcc cgctcacctg ggaagtcgac
     3421 gcgccagttc cgccgcccga gaccgcgatg taccacaccg atccgcgcgt cgcggcgagc
     3481 gacgagtcgc tggcgatggt cgacctcgtc atccccggct ttcgttcgat gcacccgatg
     3541 ggtgcgcagc tcccgcagga atgtgtgcgc atcaccgcgc tcgacttcaa gtcgatgatc
     3601 cacccgacgc agtactacgt gccgtcgtac gcgcgctggt tgctcaacga agccgacatg
     3661 tcgtctgcgt accgctggca ccgcatcttc ttgcagcact tgcagtcgaa gcatccgacc
     3721 gaccggtgga tcctgaagtc gcccgggcac atctggtgtc tcgaagcgct gctggccgag
     3781 tacccggacg cgttgctcgt gcagacgcac cgcgatccgc tccgcatcat cgcgtcgctc
     3841 ggttcgttgg tctcgacatt gcgttcgctc gcgagcagca acacgtcgat ccccgtcgcg
     3901 gccggtgagt tcgccgacta catcatcgac ggcctcgacc gttccgtgga cgcgcgcgaa
     3961 aacggcatcg tgagcgacga tcgggtcgtc gacgtgtact tcgaccagtt catggccgac
     4021 ccgttcgcgg tgatcgccaa gatctacgcc gccgccgggt tcgaatacac ggctgacgcc
     4081 gaagcgcgca tgcgcgccca cctcggcgac aacccgcagg acaagca
//
LOCUS       DASUGA010000643         2365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1312362, whole genome shotgun sequence.
ACCESSION   DASUGA010000643 DASUGA010000000
VERSION     DASUGA010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312362"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1388)
                     /locus_tag="VFX21_12740"
     CDS             complement(30..1388)
                     /locus_tag="VFX21_12740"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX5096882.1"
                     /translation="MRRRPAVTTALAFTWLLAACGGADSSDRDTVASPSNGPTASTIA
                     ATGAGGEAEYDAYADGKGVGRDDAAATERLAGPSAPGGDVATPPPISTTLNAGSVDDN
                     EAWEDFLRYRDDFLASGIPAHDVDITNRYVVTVVGDDGRPVSGAQVELRDPDGKKNAA
                     RTTYADGRVLMYPPRPSGQDTSVEYDVVVTKGDASATAALNAETREQTITLAHAAHIA
                     DPAPVDIVFLLDATGSMGDEIEQLKANMVSVAEQLDKLSPRPDVRFALTSYRDRGDEY
                     VTRTTDFTSDVGEFTETLRGVVANGGGDTPESLNAGLHDALAAPSWRDGDTIKLVFLV
                     ADAAPHLDYTDDADYAQEMVTAAERGIKIHPIASSGLDDQGEYVMRQLAQYTTGRFNF
                     LTYGADGVSPGDQTTHHVDDYSVLSLDELVVQLVKDELDALGGVSAPAGSTPTTQAQT
                     RN"
     gene            1469..>2365
                     /locus_tag="VFX21_12745"
     CDS             1469..>2365
                     /locus_tag="VFX21_12745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5096883.1"
                     /translation="MRFGLFMAPFHPTGQNPTLALERDLDLICHLDRLGFHEVWIGEH
                     HSGGYEIIASPEVFMAVAAERTKHIRLGSGVNSLPYHHPLLLADRFVLLDHLTRGRVM
                     LGCGPGQLTSDAHMLGIPPDEQRPRMDESLDAIMALLRGETVTMETDGFVLRDARLQL
                     MPYSDPTLEVAVAASISPTGARAAGKHGIGLLGIAATTAQGFDMIGTHWNTWTETAAR
                     YGHVADRAKWRLVGPMHLAETKEQARKDVEYGIVPFSKYFTKVLPAGPTRGDTPEEIL
                     GNLDEDGFAVVGTPDDAIRKIES"
BASE COUNT          347 a          814 c          812 g          392 t
ORIGIN      
        1 cggcacgacg ccgaggtacg cgctcatcgc tagtttcgag tctgggcctg cgttgtcggc
       61 gtggatccag cgggagcgct gacgccaccg agcgcgtcga gctcgtcctt cacgagctgc
      121 acgacgagct cgtccagcga cagcaccgag tagtcgtcga cgtggtgcgt cgtctggtca
      181 ccgggtgaca cgccgtcggc gccgtaggtc aggaagttga agcgaccggt cgtgtactgc
      241 gcgagctggc gcatcacgta ctcgccctgg tcgtcgagcc cgctcgacgc gatcgggtgg
      301 atcttgatgc cccgctcggc cgcggtcacc atctcctgcg cgtagtcggc gtcgtcggtg
      361 tagtcgaggt gcggtgcggc gtcggcgacg aggaacacga gcttgatcgt gtcgccgtcg
      421 cgccacgacg gtgccgcgag cgcgtcgtgg agccccgcgt tcaaactctc gggcgtgtcg
      481 ccgccgccgt tggcaacgac gccacggagg gtctcggtga attcgccgac gtcggacgtg
      541 aagtcggtcg tgcgcgtcac gtactcgtcg ccgcggtcgc gatacgacgt gagcgcgaag
      601 cgaacgtccg gccgcggcga gagcttgtcg agctgttcgg cgaccgacac catgttggcc
      661 ttcagctgtt cgatctcgtc gcccatgctg ccggtcgcgt cgagcaggaa cacaatgtcg
      721 accggtgccg ggtccgcgat gtgcgcggca tgtgccagcg tgatcgtctg ttcgcgcgtt
      781 tctgcgttca gcgcggccgt cgccgacgcg tcgcccttcg tcacgacgac gtcgtactcg
      841 acgctcgtgt cctggccgct cggtcgcggc gggtacatga gcacgcggcc gtcggcgtac
      901 gtggtgcgcg ccgcgttctt cttcccgtcg ggatcgcgca actcgacctg cgcgccggac
      961 accggccgtc cgtcgtcgcc gacgaccgtg acgacatacc gattcgtgat gtcgacgtcg
     1021 tgcgccggaa tgccggacgc gaggaagtcg tcgcggtagc gcaggaagtc ctcccacgcc
     1081 tcgttgtcgt cgacgctgcc cgcgttgagg gtcgtcgaga tcggaggggg tgtggcgacg
     1141 tcaccaccag gtgccgatgg cccggcgagg cgttcggtcg cggccgcgtc gtcgcggccc
     1201 acgcccttgc cgtctgcgta cgcgtcgtac tcggcctcgc cacccgcacc ggttgcggcg
     1261 atggtggacg ccgtcgggcc gttcgacgga ctcgccaccg tgtcgcggtc cgacgagtcg
     1321 gcgcctccgc acgctgccaa cagccaggtg aaggcgagag cggtggtgac ggcaggtcga
     1381 cggcgcatgg cgtcctccgt ggtggtgcga cctccctccg acgacccgaa tggacacgcc
     1441 ggttccccga tccgccagaa tcctcgccat gcgctttggc ctcttcatgg ctccgttcca
     1501 cccgacgggg cagaacccga cgctcgcgct cgagcgcgac ctcgacctca tctgccacct
     1561 cgaccgcctg ggcttccacg aggtgtggat cggtgagcac cactccggcg gctacgagat
     1621 catcgcctcg cccgaggtgt tcatggcggt ggccgccgag cggacgaagc acatccgcct
     1681 gggcagcggc gtcaactcgc tgccgtatca ccacccgctg ctgctcgcgg accgcttcgt
     1741 cctcctcgac cacctgacgc gcggccgcgt catgctcggc tgcggaccgg gccagttgac
     1801 atcggacgcg cacatgctcg gcatcccccc ggacgagcaa cgaccgcgga tggatgaatc
     1861 actcgacgcg atcatggccc tgctgcgcgg cgagaccgtc acgatggaga ccgacggctt
     1921 cgtgttgcga gacgcgcgct tgcagctgat gccgtacagc gacccgacgc tcgaggttgc
     1981 cgtcgccgca tcgatctcgc cgaccggtgc acgcgcggcc ggcaagcacg gcatcggtct
     2041 gctcggcatc gcggccacga cggcgcaggg attcgacatg atcggcacgc actggaacac
     2101 gtggacggag accgcggcgc gctacggaca cgtcgccgat cgtgcaaagt ggcgtctcgt
     2161 cggcccgatg cacctcgccg agacgaagga acaggcgcgc aaagacgtcg agtacggcat
     2221 cgtgccgttc tcgaagtact tcacgaaggt gttgccggcc ggaccgacgc gtggtgacac
     2281 gcccgaggag atcctcggca acctcgacga ggacggcttc gcggtggtcg gcacgcccga
     2341 cgacgcgatc cgcaagatcg agagc
//
LOCUS       DASUGA010000644         3557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1486441, whole genome shotgun sequence.
ACCESSION   DASUGA010000644 DASUGA010000000
VERSION     DASUGA010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486441"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VFX21_12750"
     CDS             complement(<1..389)
                     /locus_tag="VFX21_12750"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5096884.1"
                     /translation="MKLEGAKILVTGASSGIGAALAPILAERGATVGIVARREDRLRD
                     VLAQCREHAPDSQLWAADLGDLARAEHVAREAWDAFGGLDALVNNAAIPKRTRVTELT
                     AADITHLMDVDFHSPVRMSLALLPRMI"
     gene            complement(386..1648)
                     /locus_tag="VFX21_12755"
     CDS             complement(386..1648)
                     /locus_tag="VFX21_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017595456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HEX5096885.1"
                     /translation="MSILDTADKLFTGELSVEQHHPFASSGELAEVAPRTAFVDAFAN
                     SAVFDTDDGLVVVDTSGVFHAKGVHETVRRWSANRLDTAVFTHGHIDHVFGVELYEED
                     ARTNGWAPPRVVAHELVVDRFDRYKMTAGYNAVINQRQFKAPGLRWPTEYRYPDETYR
                     TTLTLTVADEPFELHHARGETDDGTWVWAPERRILCAGDLFIWASPNCGNPQKVQRYA
                     RDWAAAFRTMTQLDADVLLPGHGLPIVGADRVRRALTDGADLLESLLDQTLAMMNDGA
                     RLDDIVNTVRAPEHLLARPYLRPVYDEPEFVVRNIWRLYGGWYDGDPSHLKPAPANEL
                     AEELAALAGGATRLAERAREVAAAGDLRLAGHLAEMAAQAAPDDKGVHEVRAEIFGAR
                     AREEASTMSKGVFSWAEHESRQIAGSTE"
     gene            1712..2317
                     /locus_tag="VFX21_12760"
     CDS             1712..2317
                     /locus_tag="VFX21_12760"
                     /inference="COORDINATES: protein motif:HMM:NF036523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX5096886.1"
                     /translation="MVLDAAASEPAGALAKSQAARRRRVLDATLRLAADGGFDAVQMR
                     DVAAAADVALGTVYRYFSSKERLLLEAMAEQQEDLRSYLHSHPPTGTTAPERVTAVLG
                     RSNRSLRRYPDVTAAMVRAFGSAQPENADIVARVTDIMTEIITTAIHGGDQQPPTTRD
                     VRVARILMQVWLSSLNGWVCGVDGPERVDEDLAAAAELLLA"
     gene            2330..>3557
                     /locus_tag="VFX21_12765"
     CDS             2330..>3557
                     /locus_tag="VFX21_12765"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5096887.1"
                     /translation="MTPRHYNFADLFEMAADKVPDRLAVVDTRRRVTYAELEDRANRL
                     AHALQEMGVEPGQHVGILATNCIEWVEAMFAIYKIRASVVNVNFRYVEEELRYLFDNS
                     DMVALVYQREYAPLIAAARDAQPKLERFIRIEWDGDDTDDSTLDPVEYEAALAAQSGT
                     RDFAERSDDDVYLLYTGGTTGMPKGVMWRHEDVYYALAGGIDAFTGERVTRPSEASEK
                     IDASQPSGLVLMPVPPLMHGAGQMGTYRAMFEGNTAVLIDKFDAEAVWRLVERERINV
                     LTITGDAMARPLADKLEELGDAIDVSSVVSFSSTAAIFSQSVKEQLQELLPDYVVMTD
                     GIGSTESGMNGLRVVQKGDAPKEGITSVQAAADSVVLDDDYEPVAPGSGVVGRLARGG
                     NIPLGYYNDPVKSAEVF"
BASE COUNT          558 a         1221 c         1230 g          548 t
ORIGIN      
        1 tcgatcatgc gcggcaacag ggcgaggctc atgcgtaccg gggagtggaa gtcgacgtcc
       61 atgaggtgcg tgatgtccgc ggcggtcagc tcggtgacgc gcgtgcgttt cgggatcgcc
      121 gcgttgttga cgagcgcgtc gagcccgccg aacgcgtccc acgcttcccg cgcaacatgt
      181 tcggcacgcg cgaggtcgcc gaggtccgcg gcccagagtt gcgagtcggg cgcgtgttca
      241 cggcactgcg cgagcacgtc tcggagacgg tcttcgcgcc gggcgacgat gcccacggtc
      301 gccccgcgtt cggcgaggat cggcgcgagc gcggcgccga tgccggacga cgcgccggtg
      361 acgaggatct tggcgccttc cagcttcatt cggtggaccc cgcgatctgc cggctctcgt
      421 gctcggccca gctgaagacg cccttcgaca tcgtcgacgc ctcttctcgc gcccgggcgc
      481 cgaagatctc ggcccgcacc tcgtgcacgc ccttgtcgtc gggcgcggcc tgggccgcca
      541 tctcggcgag gtggccggcg aggcgcaggt cgccggcggc cgcgacctcg cgtgcgcgtt
      601 cggcgaggcg cgtggcgcca cccgcgagtg cggccagctc ctcggcgagc tcgttggcgg
      661 gcgcgggctt caggtgcgac ggatcgccgt cgtaccagcc gccgtagaga cgccagatgt
      721 tgcgcacgac gaactcgggc tcgtcgtaca caggccgcag gtacggccgg gcgagcaggt
      781 gctcgggcgc gcgcaccgtg ttgacgatgt cgtcgagacg cgcgccgtcg ttcatcatcg
      841 cgagcgtctg gtcgagcaac gactccagga ggtcggcgcc gtcggtcagt gcccgccgca
      901 cgcgatcggc gccgacgatc gggaggccgt gcccgggcag cagcacgtcc gcgtcgagct
      961 gcgtcatcgt gcggaacgcg gccgcccagt cgcgcgcgta gcgctgcacc ttctgcgggt
     1021 tcccgcagtt cggcgacgcc cagatgaaca agtcacccgc acagaggatg cggcgctcgg
     1081 gcgcccacac ccacgtcccg tcatcggtct cgccgcgcgc gtggtgcaac tcgaacggtt
     1141 catccgcgac cgtgagcgtc aacgtcgtgc ggtatgtctc gtcgggatag cggtactccg
     1201 tgggccagcg cagtcccggt gccttgaact ggcgttggtt gatgaccgcg ttgtaaccgg
     1261 cggtcatctt gtaacgatcg aagcggtcga cgacgagttc gtgcgcgacg acgcgcggcg
     1321 gcgcccagcc gttcgtgcgc gcgtcctctt cgtagagctc gacaccgaag acgtggtcga
     1381 tgtgcccgtg ggtgaagacc gcggtgtcga gccggttcgc cgaccagcgc cgcacggtct
     1441 cgtgcacacc cttggcgtgg aagacgccgc tggtgtcgac gacgacgagg ccgtcgtcgg
     1501 tgtcgaacac ggccgagttc gcgaacgcgt cgacgaacgc ggtgcggggt gccacctccg
     1561 ccagctcgcc gctgctcgcg aacggatggt gctgctcgac cgacaactca cccgtgaaca
     1621 gcttgtccgc agtgtcgagg atgctcatgg ccccgatcct ggcccagcgg gcgttgcgga
     1681 caaaatcgaa acctgttcta aaatcgcgcg gatggtgctg gacgcggccg cctccgaacc
     1741 ggctggtgca ttggccaaga gccaggcggc ccggcgccgg cgcgtgctgg acgcgacgct
     1801 gcgcctcgcg gccgacggtg gcttcgacgc ggtgcagatg cgcgacgtgg cggccgcggc
     1861 cgacgtcgcg ctgggcacgg tgtaccgcta cttcagctcc aaggagcggc tgctgctcga
     1921 agcaatggcg gagcagcaag aagacctgcg ctcctacctg cactcccatc cgccgaccgg
     1981 cacgaccgcg ccggaacggg tcactgcggt gctcggccgt tcgaaccgtt cgttgcgccg
     2041 gtatcccgac gtcaccgcgg cgatggtgcg cgcgttcggt tccgcgcaac ccgagaacgc
     2101 cgacatcgtc gcgcgcgtca ccgacatcat gaccgagatc atcacgaccg ccattcacgg
     2161 cggcgaccag cagccgccga ccacgcgcga tgtgcgcgtc gcgcgcatcc tcatgcaggt
     2221 atggctgtcg tcgctcaacg gctgggtgtg cggtgtcgac gggcccgagc gcgtcgacga
     2281 agacctcgcg gccgccgccg agctattgct cgcctgacaa ccacttctca tgacaccacg
     2341 ccactacaac tttgccgacc tgttcgagat ggccgcggac aaggtccccg accggctcgc
     2401 ggtcgtcgac acccgccggc gcgtcaccta cgccgagctc gaagaccggg ccaaccggct
     2461 cgcgcacgcc ctgcaggaga tgggcgtcga gcccggccag cacgtcggga tcctcgcgac
     2521 gaactgcatc gagtgggtcg aggccatgtt cgcgatctac aagatccgcg ccagcgtcgt
     2581 gaacgtgaac ttccgctacg tcgaggagga actgcggtac ctcttcgaca actccgacat
     2641 ggtcgcgctc gtctaccaac gcgagtacgc gcccctgatc gccgcggccc gcgacgcgca
     2701 accgaagctc gagcggttca tccgcatcga gtgggacggc gacgacaccg acgactcgac
     2761 gttggatccg gtcgaatacg aagcggcgct cgccgcgcag tcgggtacgc gcgacttcgc
     2821 cgaacgctcc gacgacgacg tctacctgct ctacaccggc ggcacgactg gtatgcccaa
     2881 gggcgtcatg tggcggcacg aggacgtcta ctacgcgctc gcgggcggca tcgacgcgtt
     2941 caccggtgaa cgagtgacgc gtccctccga ggcgagcgag aagatcgacg cgtcgcagcc
     3001 gtcgggcctc gtgctcatgc ccgtcccgcc cctgatgcac ggcgccggtc agatggggac
     3061 gtaccgcgcc atgttcgaag gcaataccgc ggtgctcatc gacaagttcg acgcggaggc
     3121 ggtgtggcgt ctcgtcgagc gcgagcgcat caacgtgctc acgatcaccg gtgacgcgat
     3181 ggcacgaccg ctcgcggaca agctcgagga gttgggcgac gcgatcgacg tgtcgtccgt
     3241 cgtgtcgttc agcagcaccg ccgcgatctt ctcgcagtcg gtcaaagagc agttgcagga
     3301 actgctgccc gactacgtcg tgatgaccga cggcatcggc tcgaccgaga gcggcatgaa
     3361 cggtctgcgc gtcgtgcaga agggtgacgc gccgaaggaa ggcatcacgt cggtgcaggc
     3421 cgcggccgac agcgtcgtgc tcgacgacga ctacgagccg gtggcacccg ggtccggtgt
     3481 cgtcggcagg ctggcccgag gcggcaacat cccgctcggc tactacaacg acccggtcaa
     3541 gagcgccgag gtgttcg
//
LOCUS       DASUGA010000645         2704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_61925, whole genome shotgun sequence.
ACCESSION   DASUGA010000645 DASUGA010000000
VERSION     DASUGA010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61925"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1722)
                     /locus_tag="VFX21_12770"
     CDS             complement(118..1722)
                     /locus_tag="VFX21_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006539448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5096888.1"
                     /translation="MARSQTTVLEVLAQRLDADPDGPYLDFEGVLYTAREMDRESNRL
                     AHALREMGVAHGDRVATLLENRAEQVVTFFAALKIGAVQVPINTAYKGEFLRHQLADS
                     GAKIFVVQGDFASRAVEVVGDTNTPDLQYVIVADPPDAVIDARATVPWQDALAAGSED
                     PITDVTVRPGDLACFIYTAGTTGPSKGCMLPHHYIVALADQIARAWQRRPDDIVLTPL
                     PLFHFNAISVCVVGSLLVGGSAAIERRFSVSNFWPEIKRTRATMVSMLGSLAILIANA
                     DDHPDQQGHQLRLCAAAPMPPDTDRAWQERFGCTTFSGGYGLTEASLVCMLDAGEPNK
                     PGAMGKPNSHEFDVRIVDDDDNEVPVGEVGEIICRPTGPNLMFLGYWNRPDATVEATR
                     NLWFHTGDLGRVDEDGYAYFVDRKKDALRRRGENISSFEMEKVLFGHDAIRDAAAHAV
                     PSPIGEDDVKITVVLSDGASLSEEDLCRWVAERVPFFAIPRYVEFRSDLPRNPVGRVL
                     KYQLRDEGVTATTWDREAAGVEFDRR"
     gene            complement(1789..>2704)
                     /locus_tag="VFX21_12775"
     CDS             complement(1789..>2704)
                     /locus_tag="VFX21_12775"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5096889.1"
                     /translation="PTDSTGQAIVGDAQYELGHLAAAEKIYVGLADRLGDAPEITARL
                     ARLNHAQGNDESAVRYAAQAEQRARAGGYNRNDLAYYDILNAELARGEGRYDDAAKEF
                     ADALELRPDYGVAIEGLGKVRAAQGRLDEAERYWKQSGKLIGAPDFHVLSALGDIELA
                     RGNEMQARQYWSQALDAVDSLSADERIGFLRDESRFRASRGLDVSFALQLAEQDFKGR
                     QDALALDTLAWAQFANGDIERARVSIDKALASGVREAFIWYHAAEIYAAGGDRDGARM
                     YVDKALDLSPHFDLYESAKAVALRATLS"
BASE COUNT          429 a          872 c          921 g          482 t
ORIGIN      
        1 ggatctggaa agcgcgcagg atcggctgag cggtcagccc tgcagcacaa acgaagcgtc
       61 agtgattctt gggagcctga tgtaccgaat cggtacacgc ggttcccaag aaccgaatca
      121 gcggcggtcg aactccacgc ccgcggcttc gcggtcccag gttgtggcag tgacaccctc
      181 gtcgcggagc tggtacttga gcacgcggcc gacgggattg cgcgggaggt cgctgcggaa
      241 ctcgacgtag cgcgggatcg cgaagaacgg cacccgttcg gccacccagc ggcacaagtc
      301 ctcctcgctg agactcgcgc cgtcgctcag cacgacggtg atcttcacgt cgtcctcgcc
      361 gatcgggctc ggcaccgcgt gcgcggccgc atcgcgaatc gcgtcgtgac cgaacagcac
      421 cttctccatt tcgaagctcg agatgttctc gccccgtcga cgcaacgcat ctttcttgcg
      481 gtcgacgaag taggcgtagc cgtcttcgtc gacgcgcccg aggtcgccgg tgtggaacca
      541 caagttgcgg gtcgcttcga cggtcgcgtc gggccggttc cagtagccga ggaacatgag
      601 gttcggaccg gtcgggcggc agatgatctc gccgacttcg ccgaccggca cttcgttgtc
      661 gtcgtcgtcg acgatgcgaa cgtcgaactc gtgcgaattg ggcttgccca tcgcacccgg
      721 cttgttgggt tcgcccgcgt cgagcatgca cacgagcgac gcctcggtta agccgtagcc
      781 gccgctaaag gttgtgcagc cgaagcgttc ctgccacgcg cgatcggtgt cgggtggcat
      841 cggcgcagcc gcgcagagcc ggagttggtg gccctgttgg tcgggatggt cgtcggcgtt
      901 ggcgatgaga atcgcgagtg agccgagcat gctgaccatc gtggcgcgcg tgcgcttgat
      961 ctccggccag aagttgctga ccgagaaccg tcgttcgatc gcggcgctgc cgccgacgag
     1021 caacgaaccg acgacgcaca cactgatcgc gttgaagtgg aacaacggca atggcgtgag
     1081 cacgatgtcg tccggccggc gctgccacgc ccgagcaatc tggtcggcaa gagcgacgat
     1141 gtaatggtgc gggagcatgc agcccttcga ggggccggtc gttcccgcgg tgtagatgaa
     1201 gcaggccaag tcgccgggcc gcacggtgac gtcggtgatc gggtcttcgc tcccggccgc
     1261 cagcgcgtcc tgccacggca ccgtcgcgcg cgcgtcgatg acggcgtcag gcggatcggc
     1321 gacgatgacg tactgcaggt cgggcgtgtt ggtgtcgccg accacttcca ccgcgcggga
     1381 cgcgaagtcg ccctgtacga cgaagatctt ggcgcccgag tcggcgagct ggtggcggag
     1441 aaactcgccc ttgtaggcgg tgttgatcgg gacctgcacc gcaccgatct tcaatgctgc
     1501 gaaaaacgtg acgacttgtt ctgcgcgatt ttcgaggagg gttgcgacgc ggtcgccgtg
     1561 cgccactccc atctcgcgca acgcgtgcgc aagccggttc gactcccggt ccatttcgcg
     1621 cgccgtgtac aggacaccct cgaagtcgag atacggcccg tccggatccg cgtcgagtcg
     1681 ctgggcgagc acttcgagaa cagtcgtctg cgatctggcc acgccgaaac aatacggggg
     1741 cgctttacag gtgcggaatt cgcacctgaa aagcgcctcc ttcgttgatc aggagagcgt
     1801 ggcgcgcagc gccaccgcct tcgcagattc gtagaggtcg aagtgggggc tcagatcgag
     1861 cgccttgtcg acatacatgc gcgcgccatc ccggtcgccg ccggccgcgt agatctcggc
     1921 cgcgtggtac cagatgaacg cttctcgcac accgctggcc aacgctttgt cgattgacac
     1981 ccgcgcgcgc tcgatgtcgc cgttcgcgaa ctgcgcccac gccagcgtgt cgagcgcgag
     2041 tgcgtcctgg cggcccttga agtcctgctc ggccagttgc agcgcgaagc tcacgtcgag
     2101 gccgcgactc gcccggaatc gcgactcgtc acggaggaac ccgatccgct cgtcggcgga
     2161 gagggaatcg accgcgtcga gcgcttgcga ccagtactgc cgcgcctgca tctcgttgcc
     2221 ccgggcgagt tcgatgtcgc cgagcgcgga gagcacgtgg aagtcgggtg cgccgatgag
     2281 cttgcccgac tgcttccagt accgctcggc ctcgtcgagg cggccttgcg cggcccgcac
     2341 cttgccgaga ccttcgatcg cgaccccgta gtcgggccgg agttcgagcg cgtcggcaaa
     2401 ctctttggcc gcgtcgtcgt agcggccttc gccgcgcgcg agttcggcgt tcaggatgtc
     2461 gtagtacgcg aggtcgttgc ggttgtagcc gccggcccgg gcgcgctgct cggcctgcgc
     2521 ggcatatcgc acggcgctct cgtcattccc ctgcgcgtgg tttagacgag cgaggcgcgc
     2581 ggtgatctcg ggcgcgtcgc cgagacggtc ggccagaccg acgtagatct tttcggccgc
     2641 agcgaggtgg ccaagttcgt actgcgcgtc accgacgatc gcttgtccgg ttgagtctgt
     2701 cggg
//
LOCUS       DASUGA010000646         5825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1450613, whole genome shotgun sequence.
ACCESSION   DASUGA010000646 DASUGA010000000
VERSION     DASUGA010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5825
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450613"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="VFX21_12780"
     CDS             complement(<1..176)
                     /locus_tag="VFX21_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEX5096890.1"
                     /translation="MNIEDVRAAAGRIAGAVVETPTTFSATLSEITGAEVWLKFENLQ
                     FTSSFKERGALNFLL"
     gene            239..823
                     /locus_tag="VFX21_12785"
     CDS             239..823
                     /locus_tag="VFX21_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5096891.1"
                     /translation="MAVAPVESTRERLIAAAIEVFVDQGYEQARVQDIARTAGLTTGA
                     IYANYRGKADLLSDAINARTAAEVDALLHDQTHGSRELLEALGDSLLRRRDAAPALLL
                     DAIAAARRDDDLAATLRDRLGSREHALADLTDAAKRNGTIDDDIDTPTFARFCTMLAM
                     GALVMRTLDTDPPDRDEWHTLLGRLLDAVSPKEK"
     gene            823..1887
                     /locus_tag="VFX21_12790"
     CDS             823..1887
                     /locus_tag="VFX21_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5096892.1"
                     /translation="MTLTNEQHDTIHAVTANMDAIFTWDYERSRPALIKLYEKAKTSQ
                     WNASTDLDWATDVDPEKVGQELGGGGVARFHALADAEGSPVKHFGDKEWLQVSVELQN
                     SLLSQFMHGEQGALLCTARIVETVPWIDAKYYAATQVMDEARHVEVFARYLDEKLGSQ
                     YKINDNLKAILDDILSDSRWDITYLGMQIMVEGLALAAFGLMHQITTEPLLKKLLRYV
                     MSDEARHVAFGVLSLQEYYKELTAAEVRERQEFAFDVATRLQRRFAQSEVWERMGVDP
                     VAVYDTMTRVNPPQQQMFQQMLFSKIVPNCKKLGLLDAADGWLRERFTEVGVIQFEDF
                     VDTGEEYALLDEVAKDREVA"
     gene            complement(1884..2357)
                     /locus_tag="VFX21_12795"
     CDS             complement(1884..2357)
                     /locus_tag="VFX21_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018849416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5096893.1"
                     /translation="MPKITPNLWFDGNAMEAAEFYCSVFPNSKIKTVTHYTDVGPGKA
                     GEVLTVEWELDGTTFVGINGGSQFKFDEAVSFEIDVKDQKELDYYWDTLTADGGEESQ
                     CGWLRDKFGLSWQVVPEDWAEIYADPDKEKVNRVMQAMFGMKKLDIAALKAAEKG"
     gene            2470..4035
                     /locus_tag="VFX21_12800"
     CDS             2470..4035
                     /locus_tag="VFX21_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5096894.1"
                     /translation="MSPTQEELLEAADKGMLPAVWARHQPAKTALWSEAGDRTFAALN
                     ANANRLVRALAARGVQPGDGIALMCSNRPEFVETLDAAQRSGLHLTTINWHLTGEEAG
                     YIVDDSDASAFVADARFADAAARAAKLAPNLKARFAVGGDIEGFERWEAALAPEDGAD
                     PDNPVVGGTMLYTSGTTGRPKGVRRKPDPRATLVAGRTTMYDAQQHVHLCTGPLYHAA
                     PLAFSLAHPMSHGVPVVLMDGWNAEDTLRLIEERGVTHTHMVPTMFHRLLSLPDDVRA
                     RYDISTLIFVIHGAAPCPVQVKQRLIEWLGPIVYEYYAATEGAGTLVGSAEWLAKPGT
                     VGRVTPPDHVRILDADGNEAPRGTAGTVYLKAPGADGSRFEYYKAPEKTAGAYSSTGD
                     YFTLGDVGYMDDDGFLFLTDRSAHLIITGGVNVYPAEVEAQLIGHPAVGDVGVVGVPD
                     AEWGELVVAAVEPQPGVEPTEELAAELIEWCRGRVAHYKCPRRIVFVEALPRHDNGKL
                     YKHQLREQLRTAN"
     gene            4049..4945
                     /locus_tag="VFX21_12805"
     CDS             4049..4945
                     /locus_tag="VFX21_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009738625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5096895.1"
                     /translation="MGICDGRVVIVTGSGRGIGREHALAYAAEGAKVVVNDLGGDTRG
                     EGGDLSPAMEVVEEIKAMGGEAVADGENVADYAGAGRMVQRAIDSFGRLDTIVNNAGI
                     LRDRMLTNMEESEWDAVIAVHLKGTFGPTKHAAAYWREQAKAGTPVNGRIVNTSSPSG
                     LFGNAGQTNYGAAKAGIAAFTIICGIELSRYNVCANAIAPVARTRMTEDLGFGAAPEG
                     KFDALDPANISPLVVWLGSNENESVNGRVFLVDGDRVSVAEGWHRGPTARNDGMRWEP
                     SQLTKVVPDLVAQAAEPSNMLS"
     gene            4958..5812
                     /locus_tag="VFX21_12810"
     CDS             4958..5812
                     /locus_tag="VFX21_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX5096896.1"
                     /translation="MPMNLDAVGSVSEPGERSWNSKDALLYALGVGAGQTDPTGFELQ
                     YTTENSQNVAQQVLPTFPVIIGMGGGAMPAFGTFNPAMLVHGEERIEVFGPIPVEGTI
                     VGTTRVLGIYDKGSGALAVLETESKYKDSGKPAFNVRFAAFIRGEGGFQSHGPEMPAR
                     PKIPERAPDHEVTYATRSDQALLYRLSGDRNPLHSDPEFAKLAGFTKPILHGLCTYGF
                     TGRALLHALCDSDPARFKVMDARFSKPVMPGDVLTVKMWVDGNDAVFQTVTQDGTVVI
                     DGGHFQSQ"
BASE COUNT         1001 a         1967 c         1966 g          891 t
ORIGIN      
        1 aggaggaagt tgagcgcgcc ccgttccttg aacgacgacg tgaactggag gttctcgaac
       61 ttcagccaga cctcggcgcc ggtgatctcg gacagcgtgg ccgagaaggt ggtcggcgtc
      121 tccacgacgg ccccggcgat ccggccggcg gcggcccgta cgtcctcgat gttcacgggg
      181 agttgtctac ctacccggcg gctttcgggc ttggataatg atcgttatca tgggagcgat
      241 ggccgtcgca ccagtggagt cgacccggga gcggctgatt gccgccgcga tcgaggtctt
      301 cgtcgaccag ggctacgagc aggcccgggt ccaggacatc gcgcggacgg ccgggctcac
      361 gacgggcgcc atctacgcca actaccgggg caaggccgac ctcctgtcgg acgcgatcaa
      421 cgcccgcacc gcggcggagg tcgacgcgct gctgcacgac cagacgcacg gatcgcgcga
      481 gctgctcgaa gcgctcggcg attccctgct gcgccggcgc gacgcagcgc ccgcgctgtt
      541 gctcgacgcc atcgcggccg cgcgccgcga cgacgacctc gcggcgaccc tgcgcgaccg
      601 cctcggcagt cgtgagcacg cgctcgccga tctgaccgac gcggcgaagc gcaacggcac
      661 gatcgacgac gacatcgaca cgccgacgtt cgcgcgcttc tgcacgatgc tcgccatggg
      721 tgcgctcgtc atgcgcaccc tcgacaccga cccacccgac cgcgacgaat ggcacacgct
      781 tctcgggcgt ctgctcgacg cggtttctcc caaggagaag tgatgacgct caccaacgag
      841 cagcacgaca cgatccacgc ggtgaccgcg aacatggacg cgatcttcac gtgggactac
      901 gagcgcagcc gtcccgcgct gatcaagctg tacgagaagg cgaagacgtc gcagtggaac
      961 gcgtccaccg acctcgactg ggcaaccgac gtcgaccccg agaaggtcgg ccaagagctt
     1021 ggcggtgggg gcgtcgcgcg cttccacgcg ttggccgacg ccgagggcag tccggtcaag
     1081 cacttcggcg acaaggaatg gctccaggtc tcggtcgagc tgcagaactc actgttgtcg
     1141 cagttcatgc acggcgagca aggcgcgctg ctgtgcacgg cgcgcatcgt cgagacggtg
     1201 ccgtggatcg acgccaagta ctacgcggcg acgcaggtga tggacgaagc ccgccacgtc
     1261 gaggtgttcg cccgctacct cgacgagaag ctcggcagcc agtacaagat caacgacaac
     1321 ctcaaggcga tcctcgacga catcctgagc gactcgcgct gggacatcac gtacctcggt
     1381 atgcagatca tggtcgaggg tctcgcgctg gccgcgttcg gtctcatgca ccagatcacg
     1441 actgaaccgc tgctgaagaa gctgctccgc tacgtgatga gcgacgaagc gcgtcacgtc
     1501 gcgttcggcg tgctctcgtt gcaggagtac tacaaggagc tcaccgcggc cgaggtgcgc
     1561 gagcgccagg agttcgcgtt cgacgtcgcg acgcgcttgc agcgccgctt cgcacagagc
     1621 gaagtgtggg agcgcatggg cgtcgacccg gtcgcggtct acgacacgat gacgcgcgtg
     1681 aacccgccgc aacagcagat gttccagcag atgctgttct cgaagatcgt gccgaactgc
     1741 aagaagctcg ggctgctcga cgcggccgac ggttggctgc gcgagcgctt caccgaggtc
     1801 ggtgtgattc agttcgagga cttcgtcgac accggcgagg agtacgcgct gctcgacgaa
     1861 gtcgcgaagg accgcgaggt cgcctaaccc ttctcggcgg ccttcaatgc ggcgatgtcg
     1921 agcttcttca tgccgaacat cgcttgcatg acgcggttga ccttctcctt gtcgggatcg
     1981 gcatagatct cggcccagtc ttcgggcacg acctgccacg acaagccgaa cttgtcccgg
     2041 agccagccgc actggctctc ctcaccgccg tcggcggtca gtgtgtccca gtagtagtcg
     2101 agctccttct ggtccttgac gtcgatctcg aacgagaccg cttcgtcgaa tttgaactgc
     2161 gacccgccgt tgatgccgac gaacgtcgtg ccgtcgagct cccactcgac cgtgagcacc
     2221 tcgccggcct tgcccggtcc gacgtcggtg tagtgggtga cggtcttgat cttcgagttc
     2281 ggaaagaccg agcagtagaa ctcggcggct tccatcgcgt tcccgtcgaa ccacaggttc
     2341 ggggtgatct tcggcatcgg gctctccctg agtcggtcgt cgcaatcgtt gtaccgaccg
     2401 ggtgcgacgg caaaagtcat cggggcacac gctcgacggc ctgaccggca cctctctaga
     2461 gtccgccggg tgagcccgac gcaggaagaa ctgctggaag cggccgacaa ggggatgctg
     2521 cccgccgtct gggcgcggca tcagcccgcc aagaccgcac tgtggtcgga ggccggtgac
     2581 cgcacgttcg cggcgctgaa cgcgaacgcc aaccgcctgg tccgggcgct cgccgcacgg
     2641 ggcgtccagc cgggtgacgg catcgcgctc atgtgctcga accggcccga gttcgtcgag
     2701 acgctcgacg ccgcccagcg gtcgggcctg cacctcacga cgatcaactg gcacctcacc
     2761 ggcgaggaag cgggctacat cgtcgacgac tccgacgcct ccgcattcgt cgcggacgcg
     2821 cgcttcgccg acgcggccgc gcgcgcggcg aagcttgcgc cgaacctcaa ggcgcgtttc
     2881 gcggtcggcg gtgacatcga gggattcgaa cgctgggaag ccgcactcgc gcccgaggac
     2941 ggcgccgatc ccgacaatcc ggtcgtcggc ggcacgatgc tctacacgtc gggcacgacg
     3001 ggccgaccga aaggtgtacg ccgcaaaccc gacccgcgcg cgacgctcgt cgccggtcgt
     3061 accacgatgt acgacgcgca gcaacacgtg catctctgca ccggtccgct gtaccacgcg
     3121 gcaccgctcg cgttctcgct cgcgcatccg atgtcgcacg gcgtgccagt ggtgttgatg
     3181 gacggctgga acgccgaaga cacgctgcgg ctcatcgagg agcgcggcgt cacgcacacg
     3241 cacatggtcc cgacgatgtt ccaccgcttg ttgtcgttgc ccgacgacgt gcgcgcccgc
     3301 tacgacatct cgacgctgat cttcgtgatc cacggtgccg cgccgtgtcc cgtgcaggtg
     3361 aagcagcggc tcatcgaatg gctgggcccg atcgtgtacg agtactacgc cgcgaccgag
     3421 ggtgcgggca cgctcgtcgg gtcggcggag tggttggcga agcccggcac ggtcgggcgg
     3481 gtgacgccgc ccgaccacgt ccgcatcctc gacgccgacg gcaacgaggc gccgcgcggc
     3541 accgcgggca ccgtgtacct gaaggcgccg ggcgcggacg gcagccgctt cgagtactac
     3601 aaggcgcccg agaagacggc cggcgcgtac tcgagcacgg gcgactactt cacgctcggc
     3661 gacgtcgggt acatggacga cgacggcttc ttgttcctca ccgaccgcag cgcgcacctc
     3721 atcatcaccg ggggcgtgaa cgtctacccg gccgaggtcg aggcgcagct gatcggccac
     3781 ccggcggtcg gcgacgtcgg tgtggtcggc gtccccgacg cggagtgggg cgagctcgtg
     3841 gtggcagcgg tcgagccgca gccgggcgtc gagccgaccg aggagctggc cgcggagctc
     3901 atcgagtggt gccgaggccg cgtggcgcac tacaagtgtc cccgccgcat cgtgttcgtg
     3961 gaggcgctgc cccgccacga caacggcaag ttgtacaagc accagctgcg ggaacagctc
     4021 cgcaccgcga actaaggacg ttcatcagat gggtatctgc gacggccgcg tcgtcatcgt
     4081 gaccggctcc ggacgaggca tcgggcgcga gcacgcgctc gcctacgccg ccgagggcgc
     4141 caaggtcgtc gtcaacgacc tcggtggtga cacgcgtggc gagggtggcg acctgagccc
     4201 ggcgatggaa gtggtggagg agatcaaggc gatgggcggc gaagccgtcg ccgacggcga
     4261 gaacgtcgcc gactacgcgg gtgcgggccg catggtgcag cgcgcaatcg attccttcgg
     4321 ccgtctcgac accatcgtca acaacgccgg catcctgcgc gaccgcatgc tgacgaacat
     4381 ggaagagagc gagtgggacg ccgtcatcgc ggtgcacctc aagggcacgt tcggcccgac
     4441 gaagcacgcc gccgcgtact ggcgggagca ggccaaggcc ggcaccccgg tgaacggtcg
     4501 catcgtcaac acgtcgtcgc cgtcgggcct gttcggcaac gcgggccaga ccaactacgg
     4561 cgcggccaag gccggcatcg ccgcgttcac gatcatctgc ggcatcgagc tgtcgcgcta
     4621 caacgtctgc gcgaacgcga tcgcgccggt ggcgcgcacg cgtatgaccg aagacctggg
     4681 cttcggtgct gcgccggagg gcaagttcga cgcgctcgac cccgcgaaca tctcgccgct
     4741 cgtcgtgtgg ctcggttcga acgagaacga gagcgtcaac ggccgcgtct tcctcgtcga
     4801 cggcgaccgc gtctcggtcg cggagggctg gcaccgcggc ccgacggccc gcaacgacgg
     4861 catgcgctgg gagccgtcgc agttgaccaa ggtcgtgccc gacctcgtcg cccaggccgc
     4921 cgaaccgtcg aacatgctga gctgaggagc cgaaccaatg ccgatgaatc tcgatgccgt
     4981 cgggtccgtc tcggagccgg gcgaacgttc gtggaactcg aaggacgcgt tgctctacgc
     5041 gctcggtgtc ggcgcgggcc agaccgatcc cacgggcttc gagctgcagt acacgaccga
     5101 gaacagccag aacgtcgcgc agcaggtgct gccgacgttc ccggtgatca tcggtatggg
     5161 cggcggcgcg atgccggcat tcggcacctt caaccccgcg atgctcgtac acggcgagga
     5221 acggatcgaa gtgttcggcc cgattcccgt tgaaggcacg atcgtcggga ccacccgcgt
     5281 gctcggcatc tacgacaagg gctcgggcgc gctcgccgtg ctcgagaccg agtcgaagta
     5341 caaggacagc ggcaagcccg cgttcaacgt gcgcttcgcc gcgttcatcc gcggcgaggg
     5401 tggcttccag agccatggcc cggagatgcc ggcgcggccg aagattcccg agcgcgcacc
     5461 ggatcacgaa gtgacgtacg cgacgcgttc cgaccaggcg ctgctctacc ggctctccgg
     5521 tgaccgcaac ccgctgcact cggatcccga gttcgcgaag ctcgcgggct tcacgaagcc
     5581 gatcctccac gggttgtgca cctacgggtt caccggccgg gcgttgctgc acgcgttgtg
     5641 cgacagcgac cccgcgaggt tcaaggtgat ggacgcacgc ttctcgaagc cggtcatgcc
     5701 cggcgacgtc ctcaccgtga agatgtgggt cgacggcaac gacgcggtgt tccagaccgt
     5761 gacccaggac ggcaccgtcg tcatcgacgg cggccacttc cagtctcaat gatcgatacg
     5821 ggcgt
//
LOCUS       DASUGA010000647         2344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1035759, whole genome shotgun sequence.
ACCESSION   DASUGA010000647 DASUGA010000000
VERSION     DASUGA010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035759"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VFX21_12815"
     CDS             complement(<1..503)
                     /locus_tag="VFX21_12815"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5096897.1"
                     /translation="MSTPEELLGDDLQTRVENGVLWLTINRADKGNAIPWYLRDALTS
                     AFRDAHANLNVRAVVLTAAGEKHFCTGADLSVPQPMRTDNPSDAPDRVVGGAINMMRG
                     GFQQLMQAMQDCEKPVIVALNGTAAGGGSMLVLAADLVIAADNAKIIQVFVRRGLVPD
                     GGVAYLLP"
     gene            complement(500..>2344)
                     /locus_tag="VFX21_12820"
     CDS             complement(500..>2344)
                     /locus_tag="VFX21_12820"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX5096898.1"
                     /translation="RKAKFAVIFAAGFSETGKEGAALEARLSSLVASGEVRLLGPNTN
                     LNAFEDFRDDLDGPSIALITQSGHQGRPVFQGQEIGVRLTHWAPTGNEVDLEFADFAR
                     YFADQPEVGVLACYIEGFKDGRTLMLAADHAATLRKPIVMVKVGKTEAGESMAASHTG
                     HLTGSDAVTNAVFRQFGVTRVDGLDELLDVSAAFARTRPKTIPAWAKAGRRPGVCVYA
                     ISGGTGAHMADVLADAGLALPDLTKATQKKLHEGLIPAYLRVSNPVDSGGPPVADARG
                     RQVLDAIVADKNVDILVIPITGAVAIFSEPFVRDIIAVSKTTKKPIHVIWGAPSGTDD
                     TYYLRLLDGGLPVYRTFNNCVRAIRAYQDYWTFAARHRSPFVNASTVVSDAAKKARSM
                     LADIEPGAALSEWQSKQLLRAYGIKTSKDVLCTSAKAAAKAAKTFGASVVMKVSSPDL
                     LHKSDVGLVQVGVEPSAAKDVYDDLLARAKKADAKARVEGVLVCEMVTGGVEVLVGVS
                     QDDLFGPVITIGSGGVLVEVFEDVTFRVPPFDAAEARRAVEELKGYRLLEGVRGAKPV
                     DVDALVDTIMKVQRLAMELSDEIKELDVNPLVVRSKGAVALDALVVKK"
BASE COUNT          363 a          816 c          777 g          388 t
ORIGIN      
        1 ggcagcaggt acgcaacacc gccgtcgggc acgaggccac ggcgcacgaa cacctggatg
       61 atcttcgcgt tgtcggccgc gatcacgaga tcggccgcga ggacgagcat cgagccaccg
      121 ccggccgcgg tgccgttcaa cgcgacgatg accggcttct cgcagtcctg catcgcctgc
      181 atgagctgct ggaacccgcc gcgcatcatg ttgatcgcgc ccccgacgac gcggtcgggt
      241 gcgtcactcg gattgtccgt gcgcatcggc tgcggcaccg agagatcggc accggtgcag
      301 aagtgcttct caccggccgc ggtcaacacg accgcgcgca cgttgaggtt cgcgtgcgcg
      361 tcgcggaatg cactggtcag tgcgtcgcgc aggtaccacg ggatcgcgtt gcccttgtcg
      421 gcgcggttga tggtgagcca cagcacaccg ttctcgaccc gggtctgcag gtcgtcgccg
      481 agcaactcct ccggggtgct cacttcttca ccaccagcgc gtcgagcgcg accgcgccct
      541 tggaccggac caccaacggg ttcacatcca gctccttgat ctcgtcggac aactccatcg
      601 cgaggcgctg caccttcatg atcgtgtcga cgagcgcgtc gacgtcgacc ggtttcgcgc
      661 cgcgcacgcc ctccaacagg cggtacccct tcagctcctc gaccgcgcgc cgggcttcgg
      721 cggcgtcgaa cggcggcacg cggaacgtga cgtcttcgaa cacctcgacc agtacgccgc
      781 ccgaaccgat ggtgatcacc gggccgaaca ggtcgtcctg ggacacgccg acgagcacct
      841 cgacgccgcc ggtcaccatc tcgcacacga gcacgccttc gacgcgcgcc ttggcgtcgg
      901 ctttcttcgc gcgtgccagg aggtcgtcgt agacatcctt ggcggcggac ggctcgacgc
      961 cgacctgcac gagcccgacg tcgctcttgt gcaacaggtc cggtgacgag accttcatga
     1021 cgaccgaagc gccgaacgtc ttggccgcct tggccgccgc cttcgcggac gtgcacaaga
     1081 cgtccttcga cgtcttgatg ccgtacgcgc gcagcaactg cttcgactgc cactccgaca
     1141 gtgccgcgcc cggctcgatg tcggccagca tcgaacgcgc cttcttcgct gcgtccgaca
     1201 cgacggtcga cgcgttgacg aagggggagc ggtgccgcgc cgcgaacgtc cagtagtcct
     1261 ggtaggcgcg aatcgcgcgc acgcagttgt tgaacgttcg gtagacgggc agaccgccgt
     1321 cgagcaggcg caagtagtac gtgtcgtcgg tgcccgatgg cgcaccccag atcacgtgga
     1381 tcggcttctt tgtcgtcttc gacaccgcga tgatgtcgcg cacgaacggc tcggagaaga
     1441 tcgcgaccgc tcccgtgatc gggatcacga ggatgtcgac gttcttgtcg gcgacgatcg
     1501 cgtcgaggac ctggcggccg cgcgcatcgg cgacgggcgg accgccgctg tcgaccgggt
     1561 tcgacacgcg caggtacgcc gggatgagac cctcgtgcag cttcttctgc gtggccttgg
     1621 tgaggtcggg aagtgccaat cccgcgtcgg cgagcacgtc ggccatgtgc gcgcccgtgc
     1681 cgcccgagat cgcgtacaca cagacgccgg gacggcgccc ggctttggcc cacgcgggga
     1741 tcgtcttcgg ccgcgttcgc gcgaacgcgg ccgacacgtc gagcagttcg tcgaggccgt
     1801 cgacgcgtgt cacgccgaac tggcggaaca ccgcgttggt caccgcgtcg gatccggtga
     1861 ggtggccggt gtgcgacgcg gccatcgatt cgccggcttc ggtcttgccg accttcacca
     1921 tcacgatcgg cttgcgtagc gtcgccgcgt ggtcggcggc gagcatcaac gtgcgcccgt
     1981 ccttgaagcc ctcgatgtag cacgcgagca cgccgacctc gggctggtcg gcgaaatagc
     2041 gcgcgaagtc cgcgaactcc aggtcgactt cgttgccggt cggcgcccag tgcgtgaggc
     2101 ggacaccgat ctcctggccc tggaacacgg gccgcccttg atgccccgac tgcgtgatca
     2161 gcgcgatcga cgggccgtcg aggtcgtcgc ggaagtcttc gaacgcgttg aggttcgtgt
     2221 tggggccgag cagccgcact tcgcccgacg ccaccagcga cgagagcctg gcttcgagcg
     2281 cggcgccttc cttgccggtc tcggagaagc cggccgcgaa gatcaccgcg aacttcgcct
     2341 tgcg
//
LOCUS       DASUGA010000648         2962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2008601, whole genome shotgun sequence.
ACCESSION   DASUGA010000648 DASUGA010000000
VERSION     DASUGA010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2008601"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VFX21_12825"
     CDS             complement(<1..379)
                     /locus_tag="VFX21_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096899.1"
                     /translation="MQRFSFLLAVLTVAATASAQFTITVPNGTASAEGSTSNAFPWGR
                     GGTGLRHVSIYDSSNFTAQGVTYPIIISQLRYRPNTGVSSLASSYPSACTVQLSTCPI
                     DQSGVVAGLPLSTYEGVDVQTCFS"
     gene            complement(640..2670)
                     /locus_tag="VFX21_12830"
     CDS             complement(640..2670)
                     /locus_tag="VFX21_12830"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX5096900.1"
                     /translation="MQRFASLLAVLALGATATAQFTITVPNGTASTEGNSSNAFPWGR
                     GGSGIRICTIYDSSNFTAQGITTPIIISQLRYRPNANASSLASTYPTGATVQLSTCPV
                     DQGAVLAGSPLSTYVGANVTTCFSGPVSWPAAAATPGPCPWLITIPFSTNFVYDPTAG
                     DLTIDTDIPIQTFTGTALQLDVQTTGSLSSRAYITTGYPTGTPAITQNHGVVVEITYV
                     PAAGLYPAFTATPLTGPTGQLVQFTDQTYTSDPGGVTSYAWDVDGDSVTDYTTQNPSH
                     TYTTEGNKTVSLTVTSAMFGTQSITKTNYISIGAVDASFTSSVLTGTLVSFTDTSTGG
                     PTSWQWDFESDGIVDDTTQNPAFFYPAAGQYTCKLTVADAFSNDSTTLTFGIGIIPVP
                     GFGSTFASTAATRGFWFQSPTRFSVITATVPNETSQAIQNVAIYRLAAAPPVYSLTAT
                     GGLEFAGLNQPAAAPIPCVVSFDAGEFVGVLGACGTTTMQNSYATPAGPFASSVLGQA
                     TTLTRFGTQFNINTTGADHPYWQEPGGAITRVILGVTACAAVPYGVSSPSGLGPAGPT
                     MRGTSLPFIGQNAVHTVTNNDALVLQLMVGGFGRLGIPVPPFGTINIGSVDLIDVMNG
                     GAPVGPGQTTWSFPVPMVPALVGQSINWQNANIVIPTGEWTLSNGIEWVIGN"
BASE COUNT          474 a          919 c         1086 g          483 t
ORIGIN      
        1 cgctgaagca cgtctggacg tcgacgcctt cgtaggtgct gagcggcagg cccgcgacga
       61 ccccgctctg gtcgatcggg caggtcgaca gctgcaccgt gcacgcactc gggtagctcg
      121 acgcgagcga cgacacgccc gtgttcgggc ggtagcgcag ctggctgatg atgatcggat
      181 aggtgacgcc ctgcgccgtg aagttcgagc tgtcgtagat gctcacgtgg cgcaggccgg
      241 tgccgccgcg gccccacggg aacgcgttgc tcgtgctgcc ctccgccgag gcagtgccgt
      301 tcggaaccgt gatggtgaac tgcgccgagg cggtcgcagc aaccgtcagg actgcaagca
      361 ggaatgagaa acgttgcatg gttctctctt ggttggtggc aatggactcc tgatgcggcc
      421 ccccggaggg gacgcgcgcg agcggcagac ggtacctgtg cggtcagtcg atggaaactc
      481 cccgcgtcct cctcgccggt tgctggtcga gcagatgcgc aacaccgcac gcgcgagggc
      541 ggaaacgcaa cgaggccgcc cctcgcagga ggagcggcct cgttcccgca gccattcgga
      601 gcgaagcgtc gtgcttcgcc cgggctggtc agggcacggc tagttgccga tcacccactc
      661 gatgccgttg gacagcgtcc actcaccggt cgggatcacg atgttggcgt tctgccagtt
      721 gatgctctga cccacgaggg ccggaaccat cgggaccggg aacgaccacg tcgtctggcc
      781 ggggccgacg ggcgcgccgc cgttcatgac gtcgatcagg tcgacgctgc cgatgttgat
      841 cgtgccgaac ggcgggaccg ggatgcccag gcgaccgaag ccgccgacca tcaactgcag
      901 cacgagcgcg tcgttgttgg tgaccgtgtg cacggcgttc tggccgatga acggcagcga
      961 ggtgccgcgc atcgtcgggc ctgccgggcc gaggcccgac ggcgaactga cgccgtaggg
     1021 gaccgcggcg cacgcggtga cgccgaggat gacgcgcgtg atcgcgccgc ccggctcctg
     1081 ccagtacggg tggtcggcac cggtcgtgtt gatgttgaac tgcgtgccaa agcgcgtgag
     1141 cgtggtcgcc tggcccagca ccgacgaagc gaacggaccc gccggcgtgg cgtaggagtt
     1201 ctgcatcgtg gtcgtgccgc aggcgccgag cacgccgacg aactcaccgg cgtcgaagct
     1261 gacgacgcag gggatgggag ccgcggccgg ctggttgagc ccagcgaact cgagaccgcc
     1321 ggtggcggtg agcgagtaca ccgggggcgc agcggccagc cggtagatgg cgacgttctg
     1381 gatcgcctgg ctggtctcgt tgggcaccgt cgccgtgatg accgagaacc gcgtcggcga
     1441 ctggaaccag aagccgcgcg tcgccgccgt gctggcgaac gtcgagccga agcccggcac
     1501 cgggatgatg ccgatgccga aggtcagcgt cgtcgagtcg ttgctgaacg cgtccgcgac
     1561 cgtcagcttg cacgtgtatt gcccggctgc cggatagaag aaggccgggt tctgcgtcgt
     1621 gtcgtcgacg atgccgtcgc tctcgaagtc ccactgccag ctggtcggac cgccggtcga
     1681 cgtgtcggtg aatgacacga gcgtgcccgt caggaccgag gaggtgaagc tcgcgtcgac
     1741 cgcgccgatc gagatgtagt tggtcttcgt gatcgactgc gtgccgaaca tggcgctcgt
     1801 cacggtcagg gagacggtct tgttgccctc ggtcgtgtag gtgtgcgagg ggttctgggt
     1861 cgtgtagtcg gtgaccgagt cgccgtcgac gtcccacgca tacgaggtga cgccacctgg
     1921 atcactcgtg taggtctggt ccgtgaactg aacgagctgc ccggtcgggc cggtgagcgg
     1981 cgtcgccgtg aaggccggat agagccccgc cgccgggacg taggtgatct cgaccacgac
     2041 gccgtggttc tgcgtgatcg caggcgtacc cgtcggatag ccggtcgtga tgtatgcacg
     2101 gctgctcagc gagcccgtgg tctgcacgtc gagctgcagc gccgtgcccg tgaacgtctg
     2161 gatcgggatg tccgtgtcga tcgtcaggtc gccggccgtc gggtcgtaga cgaagttcgt
     2221 gctgaacggg atggtgatca gccacgggca aggaccaggg gtcgccgcgg ccgcgggcca
     2281 ggacaccgga ccgctgaagc aggtcgtgac gttggcgccc acgtaggtgc tcagcggcga
     2341 gccagcgaga accgccccct gatcgaccgg gcaggtcgac agctgcaccg tggcccccgt
     2401 cgggtaggtc gacgcgagcg acgacgcgtt cgcgttcgga cgatagcgca gctggctgat
     2461 gatgatcggt gtggtgatgc cctgcgcggt gaagttcgag ctgtcgtaga tggtgcagat
     2521 gcggatgccg ctgccgccgc ggccccacgg aaacgcgttg ctggagttgc cttcggtcga
     2581 tgcggtgccg ttcggaacgg tgatggtgaa ctgcgctgtc gcggtggcgc caagagccag
     2641 cacggcaagc agtgaggcga aacgttgcat ggattgtccc ttttgaggtt gttgcgtgcg
     2701 tcccggtcgc ggggcgcacc cggttggaag gaagggtatc agcatggaga gtcaatgaaa
     2761 gacccgcgtt tcgcgaacat gctgacgcgt cgcaccgcga ttgcggaatg cgtaggaccg
     2821 cagacgcggt cccgcgcgac gccgtcggcg cggaatgacc gccgccatcg aggagccatg
     2881 cgcagcgatg cggagccgcg ccccggtccg cttccccaac gcaacgaggc cgcacctcac
     2941 ccgaggcgcg gcctcgctct tc
//
LOCUS       DASUGA010000649         2341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2008638, whole genome shotgun sequence.
ACCESSION   DASUGA010000649 DASUGA010000000
VERSION     DASUGA010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2008638"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VFX21_12835"
     CDS             complement(<1..559)
                     /locus_tag="VFX21_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096901.1"
                     /translation="MTLACLLGLLVAIPGSATLAPSTFDAKDGNLLLNDANDVGSVPN
                     ANVDAKDWENAPGLRPATDRPTGQTDNSMSEGTKEDAPVPTLDVGSIPNNKSDLRHFY
                     VSNERVGGHEFLYLAWTRANTLGTANMDFEFNKNKCELTGNPPAPTDDSTCSANGTTP
                     VRSAGDMLITFDFANGGNNVNLGLAL"
     gene            complement(775..1575)
                     /locus_tag="VFX21_12840"
     CDS             complement(775..1575)
                     /locus_tag="VFX21_12840"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5096902.1"
                     /translation="MGRAAVNLAAVFGLLYLFAPIFVIVLFSFNKPKGRFNAVWQEFT
                     LDNWLHPFKDEKLVEALLLSLRVAFISTAIATVLGTFIALALVRYRIRGGEPVNFLLI
                     LPLTAPEIVLGASLLTLFLQPTLLLFNHDFTLGVTTVIIAHVMFLVSYVAVTVRARVR
                     GVDWTLEDAAMDLGAPPTRTFWRVTLPLITPGILAAALLSFALSIDDFIITYFVSGPD
                     TTTFPVRIFNQSRTATPPQINVLSTMILLVSVTVLAAGTAIGALRSRD"
     gene            complement(1608..>2341)
                     /locus_tag="VFX21_12845"
     CDS             complement(1608..>2341)
                     /locus_tag="VFX21_12845"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5096903.1"
                     /translation="LDRFSDPVFDWHWENYSEAFTQFGDQFYRSFLYGFIATVLALLI
                     GYPLAYVIAFRGGRFKNLLLGLVVVPFFTNFLIRTLAWKTLLGNESPVTHFLRAINLV
                     GDTDTLLRTPIAVIGGLTYNFLPFMVLPIYVAIEKIDPGLVDAARDLYSNTWRAFRKI
                     VFPMSLPGVFAGSLLVFIPATGDFVNAYYLGSNRTTMIGTVVQNQFLAEGDFPVAAAL
                     SFVFMTIVMVLVIVYAGILGTEDLT"
BASE COUNT          440 a          725 c          794 g          382 t
ORIGIN      
        1 aaagagcgag accgaggttg acgttgttgc cgccgttcgc aaagtcgaac gtgatcagca
       61 tgtcgcccgc gctccgcacc ggcgtggtgc cgttcgcgga gcaggtcgag tcatccgtcg
      121 gtgccggcgg attgccggtc aactcgcact tgttcttgtt gaactcgaag tccatgttcg
      181 cggtgccgag cgtgttcgcg cgggtccacg cgagatacag gaactcgtgg ccgccgacgc
      241 gttcgttcga cacgtagaag tgccgcaggt cgctcttgtt gttcgggatc gagccgacgt
      301 cgagcgtcgg gaccggggcg tcctctttcg tgccctccga catggagttg tccgtctgcc
      361 cggtcggccg gtcggtcgcg gggcgcagcc cgggcgcgtt ctcccagtcc ttcgcgtcga
      421 cgttcgcgtt cggcacgctg cccacatcgt tcgcgtcgtt cagcaacagg ttgccgtcct
      481 tcgcatcgaa cgtgctgggc gccagcgtcg ccgatcccgg tatggcgacg agcagtccca
      541 gcagacacgc gagcgtcaca aagctcgcga aagccttcct ccggcgcatc cttgcaacct
      601 ccacctcgat atcgcggtca gtgaccgcat ggtcgttgga gggcccgcca ttgccactcc
      661 ttgtgggcgc catctaggcc atacgaccta gcgtgatgtc cagtcacaat ccgtcgcgtt
      721 cctcgttcgc ctcgttcgcc cagacggtgc gtcggacaga tcgaggcgaa tcgcttagtc
      781 gcgcgagcgc aaggcgccga tcgcggtgcc ggcggccaac accgtcacgc tcaccagcag
      841 gatcatcgtc gagagcacgt tgatctgcgg cggggtcgcg gtgcgcgact ggttgaagat
      901 ccgcaccggg aacgtcgtcg tgtcggggcc gctcacgaag tacgtgatga tgaagtcgtc
      961 gatgctgagc gcgaacgaca gcagtgcggc cgcgaggatg ccgggcgtga tgagcggcaa
     1021 cgtcacccgc cagaacgtgc gcgtcggcgg cgcgccgagg tccatcgccg cgtcctcgag
     1081 cgtccagtcg acgccgcgca cgcgcgcccg aacggtcacc gcgacgtaac tcacgaggaa
     1141 catcacgtgg gcgatgatca ccgtcgtgac acccagcgtg aagtcgtggt tgaacaacaa
     1201 gagcgtcggc tggaggaaca gcgtgagcaa cgaggcgccg agcacgatct ccggtgccgt
     1261 caacgggagt atcagcagga agttgacggg ctcgccgccg cggatgcggt agcgcacgag
     1321 cgcgagcgcg atgaacgtgc cgagcaccgt cgcgatcgcg gtcgagatga acgccacccg
     1381 cagactcagc agcagcgctt cgacgagctt ctcgtccttg aaggggtgca gccagttgtc
     1441 gagcgtgaac tcctgccaca ccgcgttgaa gcggcccttg ggtttgttga agctgaagag
     1501 cacgatgacg aagatcggcg cgaacaggta gagcagcccg aacacggccg cgaggttgac
     1561 cgcggcgcga ccgatgcggc cgccgggcga ggtgaccgtg cccgagctca cgtcaggtcc
     1621 tcggtcccga ggatcccggc gtagacgatg acgagcacca tcacgatcgt catgaacacg
     1681 aacgacagcg cggccgcaac agggaagtcg ccctcggcga ggaactggtt ctgcacgacc
     1741 gtgccgatca tcgtcgtgcg gttgctcccg aggtagtacg cgttgacgaa gtcgccggtc
     1801 gcggggatga agacgagcaa cgagcccgcg aagacgcccg gcaacgacat cgggaagacg
     1861 atcttgcgga acgcgcgcca ggtgttcgag tacaggtcgc gtgccgcgtc gaccaggccg
     1921 gggtcgatct tctcgatcgc cacgtagatc ggcaggacca tgaatggcag gaagttgtac
     1981 gtgaggccgc cgatcaccgc gatgggtgtg cgcaacaacg tgtcggtgtc gcccacgaga
     2041 ttgatcgcgc gcaggaagtg ggtgacggga ctctcgttgc cgagcagcgt cttccacgcc
     2101 agcgtgcgga tgaggaaatt ggtgaagaac gggacgacga cgaggcccag caacaagttc
     2161 ttgaaccgcc cgccacggaa cgcgatgacg tacgcgagcg ggtagccgat gagcagcgcg
     2221 agcactgtcg cgatgaagcc atagaggaac gaccggtaga actggtcgcc gaactgcgtg
     2281 aacgcctcgc tgtagttctc ccagtgccag tcgaagacgg gatcggagaa ccggtcgagc
     2341 c
//
LOCUS       DASUGA010000650         4087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1312409, whole genome shotgun sequence.
ACCESSION   DASUGA010000650 DASUGA010000000
VERSION     DASUGA010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312409"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VFX21_12850"
     CDS             <1..525
                     /locus_tag="VFX21_12850"
                     /inference="COORDINATES: protein motif:HMM:NF019903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5096904.1"
                     /translation="ETRLAILRKKAEAEHLDGIPDAVLAFIADNVVDNVRELEGSLIR
                     VAAYSSLHRVPLSEDVAKDVLADLLPASRPRVITPQLILEETAKMFGWTVEELCGKSR
                     RRPLVTARQIGMYVFRELTDYSYPKIAEEFGGRDHTTVMHACEKIRAQMAEKRVVFEQ
                     VNDLISRIKHGGSG"
     gene            733..1827
                     /gene="dnaN"
                     /locus_tag="VFX21_12855"
     CDS             733..1827
                     /gene="dnaN"
                     /locus_tag="VFX21_12855"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX5096905.1"
                     /translation="MKFRCERDTLAEAVATAQRTVASRSGALPVLQDLRITATDMGLE
                     LIGSDLEITNRVQVPAEVEETGVAVVPKLLGEIVRKLEPGPVTVAVTGDEATVTAGRF
                     STSLRLKPAEDYPRLAPTDGSGVRVEAAAFAAALKQVVRAASKDDLRPILTGVLLTAH
                     AGGLRLVATDSYRLAVRDLKGVSMLDEGQRVLVAAKGLSEVQRLAGDGEIEVVLRERD
                     VVFRTSRAEVTARLIEGDFPNYEQLIPSGYPNRLTVGREALIESLDRVQIVGQSRDNA
                     AVRLAMSGEGLELSMSAQDVGNAQEAMDAKFEGSELTVAFNPMFLRDGADAIEGDEVA
                     LETIDPLKPATLRSAEGGDFLYLLMPVRTS"
     gene            1824..2966
                     /gene="recF"
                     /locus_tag="VFX21_12860"
     CDS             1824..2966
                     /gene="recF"
                     /locus_tag="VFX21_12860"
                     /inference="COORDINATES: protein motif:HMM:NF000822.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEX5096906.1"
                     /translation="MIEAPPDAAAASRVGHLWLTDFRVFREIDLELAPGLTVLFGANG
                     QGKTSLLEAISWVARARSFRGVGDAMLVRAGEAQAILRAEVYTGERQQLFEAEIRASG
                     RNRIQCNRQAVTRARDLHGLLRVTVFAPDDLALVKSGPADRRDYLDELLAMLAARYDA
                     ARSDYERVLKQRNALLRSGMRDPDAQTTLDVFDEQLAAAGAEITRGRLRLLERLIPAV
                     AHAYRTFATETREVGGEYTSDWAGGVLTEADADDLEARLRNAIADRRRAEIDRGLTLV
                     GPHRDELRLTIGGLDARHQASQGEQRTLALALRLAGHEVVRDLTGAAPVLLLDDVFSE
                     LDAARSSALVHNLPPGQTLVTTAGVLPPDVVAQRTLVVHDGTVDTR"
     gene            2963..3283
                     /locus_tag="VFX21_12865"
     CDS             2963..3283
                     /locus_tag="VFX21_12865"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEX5096907.1"
                     /translation="MTKRRDDLVRLGDAVERLTKELGIPAPGEQRSVDEVWPEVVGAA
                     LAAHARVRSIRNGECVVEVDGPAYATQLRYLGETLSAGVNERCGQVLVTSVRIVVSRA
                     HDGT"
     gene            3439..>4087
                     /locus_tag="VFX21_12870"
     CDS             3439..>4087
                     /locus_tag="VFX21_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5096908.1"
                     /translation="MAYGAKDITILKGLQPVRERPGMYIGSTGPSGLHHLVYEVVDNA
                     VDEALAGHASRIDVTLLADGGCRVIDDGRGIPVDPHPEYTDKSAAEIVLTMLHAGGKF
                     GGEGYKISGGLHGVGVSVVNALSRRLELEIWRDGGHYRQVFLDGGTPEAPLERLGDSD
                     DTGTSVTFWPDPTIMEEVDFRAQTLLERLREMAFLNKGLEIVFRDERQDPPTEQVF"
BASE COUNT          711 a         1342 c         1397 g          637 t
ORIGIN      
        1 gaaacccgcc ttgcgatcct gcgcaaaaag gccgaagccg aacacctcga tggcattccc
       61 gacgcggtcc tggcgttcat cgccgacaac gtcgtcgaca acgtccggga actcgaaggg
      121 tcgctcatcc gggttgcggc gtactcgtcg ttgcaccgcg ttccgctcag cgaagacgtc
      181 gccaaagatg tgctggccga cctcttgccg gcgtctcgcc cgcgggtgat caccccgcag
      241 ctcatcctcg aggaaaccgc caagatgttc ggctggaccg tcgaggaact ctgcggcaag
      301 agccgtcgcc gcccgctcgt caccgcccgc cagatcggga tgtacgtctt ccgggagctc
      361 accgactaca gctatcccaa gatcgccgaa gagttcggcg gccgcgatca cacgaccgtg
      421 atgcacgcgt gcgagaagat ccgggcccag atggccgaga agcgagttgt gttcgagcag
      481 gtcaacgacc tgatcagccg gatcaagcac ggcgggagtg gataacgctg tggataatgg
      541 gggaaatccg tcgacacgcg ggggagaagc agtcgggagg cgggttgtgc gcctcatgcc
      601 tgttcacgac ggtggaagag cttgtgaatc tccaagggca ctcggagcag gggtgacgag
      661 cgttcatcca caatccacag cccctactac tgcaaagacc atgaatacga acatggtgtt
      721 gaggaggaca gcgtgaagtt ccggtgcgag cgagacactc tcgccgaggc ggtggccacg
      781 gcccagcgaa ccgttgcgag ccgcagcggg gccttgccgg tactgcaaga cctccgcatc
      841 acggcaaccg acatggggtt ggaactcatc ggttccgact tggagatcac gaaccgggtg
      901 caagttcccg ccgaggtcga agagaccggc gtcgcggtgg tgccgaagct gttgggcgag
      961 atcgtgcgca agctcgagcc gggaccggtc acggttgcgg tgaccggtga cgaagccacg
     1021 gtgactgcgg gccgcttctc gacgtcgctg cgcttgaagc cggccgagga ctatccgcgt
     1081 cttgcgccca ccgacggcag cggcgtgcgc gtggaagccg ccgccttcgc ggccgcgctg
     1141 aagcaggtcg tgcgggcggc gtcgaaggac gacttgcgcc cgatcctcac cggtgtgctg
     1201 ctcactgcgc atgccggcgg cctgcgactg gttgcgaccg actcgtaccg cctggccgtg
     1261 cgcgacctca aaggcgtgag catgctcgac gaagggcagc gggtgctcgt tgccgccaaa
     1321 ggtttgtcgg aagtgcaacg cctcgccggt gacggtgaga tcgaagtcgt gttgcgggag
     1381 cgagatgtcg tgttccgcac gtcgcgcgcc gaggtcactg cgcgactcat cgaaggcgac
     1441 tttccgaact acgaacagct catcccgagt ggctacccga accggctgac cgtgggccgc
     1501 gaagcgttga tcgagtcgct cgatcgcgtg cagatcgtcg gccagagccg cgacaacgcg
     1561 gccgtgcggt tggcaatgag cggcgagggc ctcgagctgt cgatgtcggc ccaagacgtc
     1621 ggcaacgcgc aagaagcaat ggacgcgaag ttcgaaggca gcgaactcac cgttgcgttc
     1681 aacccgatgt tcctgcgcga cggcgccgac gcgatcgagg gcgacgaagt tgcgctcgag
     1741 accatcgacc cgttgaagcc ggccacgttg cgttcggccg agggcggcga tttcctctac
     1801 ctgctgatgc ccgtacgcac gtcgtgatcg aagccccgcc cgacgcggcc gccgcgtcgc
     1861 gcgtcgggca cctgtggctc accgatttcc gggtctttcg cgagatcgac ctcgaactcg
     1921 cgcccggcct caccgtgctg ttcggtgcga acgggcaagg caaaaccagc ttgctggagg
     1981 cgatcagttg ggtcgcgcgt gcgcggtcgt tccgcggcgt cggagacgcg atgctcgtgc
     2041 gcgcgggcga agcacaagcg atcttgcggg ccgaggtgta caccggtgag cggcagcagt
     2101 tgttcgaagc cgagatccgc gcgtccggcc ggaaccgcat tcagtgcaac cggcaagcgg
     2161 tcacgcgggc gcgcgatctg cacggcctgt tgcgtgtcac cgtgttcgcg ccggacgacc
     2221 tcgccctcgt caagagcgga cccgccgacc ggcgcgacta cctcgatgaa ctgctcgcga
     2281 tgctggccgc gcgctacgac gcggcgcgca gcgactacga acgcgtactg aagcagcgca
     2341 acgcgctgtt gcggtcgggc atgcgcgacc cggatgcgca aacaacgctc gatgttttcg
     2401 acgagcaact cgcagcagcc ggcgccgaga tcactcgggg ccgcctccga ctgctcgagc
     2461 gcctgattcc ggcggtcgcg cacgcctacc ggacgttcgc gaccgagacg cgcgaggtcg
     2521 gcggtgaata cacgagcgat tgggccggcg gcgtcctcac cgaagccgac gccgacgatc
     2581 tcgaggcgcg cctgcgaaac gcgatcgctg accggcgccg agccgagatc gaccgcggcc
     2641 tcacgctcgt cggaccgcac cgcgacgaac tccgattgac gatcggcggc ctcgacgcac
     2701 gccaccaggc gtcgcaaggg gagcagcgca cgctcgcgtt ggcgctgcgg ctcgccggcc
     2761 acgaagtcgt gcgtgacctc acgggggcgg ctcctgtcct gctgctcgac gacgtcttca
     2821 gtgagctcga tgcggcccgc tcgagcgcgc tcgtgcacaa cctgccgccg ggacagacgc
     2881 tggtgacgac ggccggtgtc ctgccgcccg atgtggtcgc ccaacgcacg ctcgtcgtgc
     2941 acgacggcac ggtggacacg cgatgacgaa gcggcgcgac gacttggttc gcctcggcga
     3001 cgcggtcgag cggctcacga aggagctcgg aattcccgca cccggggaac agagatccgt
     3061 cgacgaggtg tggcccgagg tcgtcggcgc ggccctcgcg gcgcacgccc gggtgcgctc
     3121 gatccgcaac ggcgaatgcg tcgtggaagt cgacggcccg gcctatgcga cgcagctgcg
     3181 gtacctcggc gagacccttt cggcgggggt caacgagcgg tgcggacagg tgctcgtgac
     3241 ctcggttcgg atcgtcgttt cgcgggctca cgacggcacc tgaaatccgg tgcgaaaccc
     3301 caggtcacac gggtcccgaa gcgacggttt cgggccccga ttctggtaga gtggggatac
     3361 cgaaaacacc ccctctgacc aggactttcg cgtgccggga cggggtgttg cagcacgctg
     3421 aacgagggat tctcgcgagt ggcgtacggc gccaaagaca tcacgatttt gaagggcctc
     3481 cagccggtcc gtgaacggcc cggcatgtac atcggatcca cgggtccgag cggcctccat
     3541 cacttggttt acgaggtcgt cgacaatgcg gtcgacgagg ccctcgcggg tcatgcgtcg
     3601 cgcatcgacg tgaccttgct ggccgacggc ggctgccgcg tcatcgacga cggtcgtggc
     3661 atcccggtcg atccgcatcc ggagtacacc gacaagtccg cggccgagat cgtgctcacg
     3721 atgctccacg ccggtggcaa gttcggcggc gagggctaca agatctccgg cggcctgcac
     3781 ggcgtcggcg tgtcggtggt gaacgcgctt tcgcgccggc tcgaactcga aatctggcgc
     3841 gacggcggcc actaccggca ggtcttcctg gacggcggga cccccgaggc gccactcgag
     3901 cgcctcggtg actccgacga cacgggaaca tccgtcacgt tctggcccga ccccacgatc
     3961 atggaggagg tcgactttcg cgcccagacg ctgctcgagc gcctgcgcga aatggcgttc
     4021 ctcaacaagg gcctcgagat cgtcttccgc gacgagcgcc aggacccgcc gaccgagcag
     4081 gtcttca
//
LOCUS       DASUGA010000651         3149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_412966, whole genome shotgun sequence.
ACCESSION   DASUGA010000651 DASUGA010000000
VERSION     DASUGA010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412966"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFX21_12875"
     CDS             <1..522
                     /locus_tag="VFX21_12875"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5096909.1"
                     /translation="DDRVYALAGIAPSIHVPSVTPWAAAAKTPALVWDVGSSSVHALT
                     DRFTPNGIREYIDAVSGKSDDDTARFVSPVGFAGLANDAVDAGWVNVFILLIGINVFV
                     GIFNLVPLLPFDGGHIAIACYEKVMSMITRRRVQVDVAKLLPITALVVGVLAIIFFTS
                     VFMDIARPMDSPF"
     gene            532..1737
                     /gene="ispG"
                     /locus_tag="VFX21_12880"
                     /gene_synonym="gcpE"
     CDS             532..1737
                     /gene="ispG"
                     /locus_tag="VFX21_12880"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX5096910.1"
                     /translation="MIKRRPTRQLMVGSVAVGGDAPVSVQSMTTTKTADVEGTLAQVY
                     ALAAAGCDIVRCTCNEEEAAEGLAQIVPRSPVPIVADIHFQHKLALAAMEAGVHALRL
                     NPGNIRKPEHIKAVAREAKDRALPIRIGVNAGSLDPAMAERHGGITPEALVASAMHEL
                     DLFREVDFEDVKISVKASNVALMIDAYRLLSETTDHPLHLGVTEAGPLPQGLIKSTAG
                     IATLLAEGIGDTIRFSLTADPVEEAKAGRVLCESLGLRERKGLDLIACPSCGRAEVDV
                     IRVASEAQAQLEGRNIPLQVAVMGCVVNGPGEAREADLGIAAGRGKGHLFVKGHVVRV
                     VPEAEMVAALIDEAEKIMSEGIEARLAAADENAEAIAAATREELLQIQGLDVNKSAEK
                     IERIRHIAE"
     gene            1734..2186
                     /locus_tag="VFX21_12885"
     CDS             1734..2186
                     /locus_tag="VFX21_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019523249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5096911.1"
                     /translation="MKIRDAQPDDLRALSWCAYHSKAHWGYDEAFMAACVDELTIRPE
                     HLATHVLRVAPRGSEVAGFHGVVLDGDDSELEWLFVAPDAMGHGIGRALLGDACAIAR
                     HAGVRQLRIESDPFARAFYERCGAVCVGEVASASIPGRVLPLLTLGVS"
     gene            2191..2907
                     /locus_tag="VFX21_12890"
     CDS             2191..2907
                     /locus_tag="VFX21_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020128265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5096912.1"
                     /translation="MAEAVTGTPVGRRVFLGLVGLGAAGVLVGARVQDWLERVVSPYV
                     SKDGSGLSSLLPIGRFRIYTVTGGLPHKSKEDWRMKVGGLVDQPYELTYAELTAMTPT
                     NLTKDFQCVTGWRVSDVKWRGVKLADLLDRAGVQSEATALRFTSFDGLYTESLTLEQA
                     RRGDIIVAYQMEGGDISRAHGGPVRLYVAPMYGYKSIKWLDGIDATKEVEPGYWEQLG
                     YDVDGWVGRSNGRDDDPTSA"
     gene            2904..>3149
                     /locus_tag="VFX21_12895"
     CDS             2904..>3149
                     /locus_tag="VFX21_12895"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX5096913.1"
                     /translation="MSRVRRFDKTERVVHWVNATLFLVLLFTGFTLYAGPLSEIVGRR
                     HLVKDIHVYAGLALPVPILLGIALRSGRALRADLRTLN"
BASE COUNT          513 a         1050 c         1081 g          505 t
ORIGIN      
        1 gacgaccgtg tctacgcgct ggccggaatc gctccgagca tccacgtccc gagcgtgact
       61 ccgtgggcgg cggcggcgaa gacgccggcg ctggtgtggg acgtcgggag cagctccgta
      121 cacgcgctca ccgaccgctt cacgcccaac ggcatccgcg aatacatcga cgcggtgagc
      181 ggcaagagcg acgacgacac cgcccgtttc gtgtcgcccg ttggcttcgc cggtcttgcg
      241 aacgacgcag tcgatgccgg ctgggtcaac gtgttcatct tgttgatcgg catcaacgtg
      301 ttcgtcggca tcttcaactt ggtgccgctg ctgcccttcg acggtgggca catcgcgatc
      361 gcgtgctacg agaaggtcat gtcgatgatc acgcgccggc gggtgcaggt cgacgtcgcg
      421 aaattgctgc cgatcacggc actggtcgtc ggcgtgctcg cgatcatctt cttcacgagc
      481 gtcttcatgg acattgctcg cccgatggac agtccgttct aacctgacgt cgtgatcaaa
      541 cgccgcccga cccgccagct catggtcggc agtgtcgccg tcggcggcga cgcgccggtc
      601 tcggtgcagt cgatgacgac gacgaagacc gccgacgtcg aaggcacgct ggcgcaggtc
      661 tacgcgctgg cggccgcggg ctgcgacatc gtgcgctgca cctgcaacga agaagaagcg
      721 gccgaagggc tcgcgcagat cgtgccccgt tcgccggtgc cgatcgtcgc cgacatccac
      781 ttccagcaca agctcgcgct cgcggcgatg gaggccggcg tgcacgcgct gcgcctcaac
      841 ccgggcaaca tccgcaagcc cgagcacatc aaggccgtgg cgcgggaggc gaaggatcgc
      901 gcgctgccga tccgtatcgg tgtcaacgcc ggttcgctcg accccgcgat ggccgaacgg
      961 cacggtggca tcacgcccga ggcgctcgtt gcgtccgcga tgcacgagct cgacctgttc
     1021 cgagaggtcg acttcgagga cgtgaagatc tcggtcaagg cttccaacgt cgcgctgatg
     1081 atcgacgcgt accgtctcct gtcggagacg accgaccatc cgttgcacct cggcgtcacc
     1141 gaggcggggc cgttgccgca agggctcatc aagtcgaccg cggggatcgc gacgctgctc
     1201 gccgagggga tcggtgacac gatccggttc tcgctgaccg cggacccggt cgaagaagcg
     1261 aaagcgggac gcgtgctgtg cgagtcgctc ggtctgcgcg aacgcaaggg actcgacctc
     1321 atcgcgtgcc cgtcgtgcgg gcgcgccgag gtcgacgtca tccgcgtcgc atcggaggcg
     1381 caggcgcagc tcgaaggccg caacatcccg ctccaggtcg cggtcatggg ctgcgtcgtg
     1441 aacggtcccg gtgaggcacg cgaagccgac ctcgggatcg cggccggccg cggcaaaggt
     1501 cacctcttcg tcaagggcca tgtcgtgcgc gtcgtaccgg aggccgagat ggtcgccgcg
     1561 ctcatcgacg aggcggagaa gatcatgagc gaaggcatcg aagcccgtct cgcagcggcc
     1621 gacgagaacg cggaggcgat cgcggccgcg acgcgcgagg agctcttgca gatccaaggg
     1681 ctcgacgtga acaagtcggc ggagaagatc gaacgcatcc gccacatcgc ggagtgaaga
     1741 tccgcgacgc gcaacccgac gacctgcgcg cgctcagctg gtgtgcgtac cactccaagg
     1801 cacactgggg ttacgacgaa gcgttcatgg ccgcatgcgt cgacgagctg accatccgtc
     1861 cggagcatct cgcgacacat gtgttgcgcg tcgcgccgcg ggggagtgag gtcgccggct
     1921 tccacggtgt cgtcctcgac ggtgacgaca gcgagctcga gtggctgttc gtcgcgccgg
     1981 acgcgatggg ccacggcatc ggccgcgcgc tgctcggcga cgcctgcgcg atcgctcggc
     2041 acgcgggtgt acggcagctg cgcatcgaat ccgatccgtt cgcccgggcc ttctacgaac
     2101 ggtgcggtgc ggtgtgcgtc ggcgaggtcg cgtcggcctc gattcccggt cgcgtcctgc
     2161 cgctgctgac cctcggcgta tcctgacctc gtggccgaag ccgtcaccgg gactccagtc
     2221 ggccggcgtg tcttcctcgg gctcgtcgga ctcggcgcgg caggtgtgct cgtcggtgcg
     2281 cgggtgcagg actggctcga acgggtcgtc agcccctatg tctccaagga cggcagcggg
     2341 ctcagctcgc tgctgccgat cgggcgcttc cgcatctata cggtcaccgg cggactcccg
     2401 cacaagtcga aggaagactg gcggatgaag gtcggcggcc tcgtcgacca gccgtacgag
     2461 ctcacgtacg cggagctcac cgcgatgacg ccgacgaacc tcaccaagga ctttcagtgc
     2521 gtcaccggct ggcgggtctc cgacgtcaag tggcgaggcg tgaagctcgc cgacctgctc
     2581 gaccgcgcgg gcgtgcagag cgaagcgacc gcgttgcgct tcacgtcgtt cgacggtctc
     2641 tacaccgaga gtctcacgct cgaacaagcg cgccgcggcg acatcatcgt cgcgtatcag
     2701 atggaaggcg gcgacatctc gcgcgcgcac ggcgggccgg tgcgcctcta tgtcgcgccg
     2761 atgtacggct acaagtcgat caagtggctc gacggcatcg acgccacgaa agaagtcgaa
     2821 cccggttact gggagcagct cggttacgac gtcgacgggt gggtcggccg cagcaacggc
     2881 cgcgacgacg atccgacgag cgcgtgagca gagtccgacg gttcgacaag accgaacgcg
     2941 tcgtgcactg ggtgaacgcc acgctgttcc tcgtgctgtt gttcacgggc ttcacgctct
     3001 acgcggggcc gctgtcggag atcgtcggcc gccggcatct cgtgaaggac atccacgtct
     3061 acgcggggct ggcgctcccg gtcccgatcc tgctcggcat cgcgctgcgg tcgggacgcg
     3121 cgctgcgcgc cgacctgcgc acgctcaac
//
LOCUS       DASUGA010000652         2458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_914654, whole genome shotgun sequence.
ACCESSION   DASUGA010000652 DASUGA010000000
VERSION     DASUGA010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914654"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VFX21_12900"
     CDS             complement(<1..661)
                     /locus_tag="VFX21_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007726692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5096914.1"
                     /translation="MAAEVVIEEPSTAPDRAPLVLITLVLVAAVANLNLAVANVALPD
                     IGKAFDASQTSLNLIAVGYSLGLAASVLYLGALGDRYGRKMMCVAGVALSIPACLIAA
                     YAPNEYVLIGARILGGLSAGMAYPTTLALITALWSGPGRTKSIALWSALGGGISALGP
                     LVSGALLEHFWWGSVFIVTLPLAAVALVLALRFIPAHVNETTEKVDNLGGVLSIALVG
                     SL"
     gene            complement(646..2046)
                     /gene="zwf"
                     /locus_tag="VFX21_12905"
     CDS             complement(646..2046)
                     /gene="zwf"
                     /locus_tag="VFX21_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX5096915.1"
                     /translation="MTTVDPVTAPADALVVFGISGDLARKMTFRALYRLERTNKLNCP
                     IIGVSLDDWSDDFLRDHARTSIVASGETLDETVFARLAARLSMIVGNYADHETFVKVG
                     KALEGKKHPVFYLEIPPSLFGTVVDGLASVGATENARVIVEKPFGHDLASAKELNAQL
                     SKHLHEWQILRIDHFLGKEPALDILFLRFANSIVEPLWNRDRIECVQITMAENFGCED
                     RGSFYDPVGAMRDVVQNHLFQLVSLIAAEPPASPNADHLRNQHVDVFRGIPAVQPDHY
                     VRGQYEGYRSVPGVKPDSDTETFCALRLNIENWRWSGVPFFLRAGKAMAVRNTEVRVV
                     FKRPPHLALAPNFNPEPNQLVMRIDPNPGSQMLVQVKAPGTRATKTVDLSVVFAEELG
                     DAPEPYERLLGDALHGDVILFTREDLVEETWRIVQPLIDNPPPVEMYAQGSWGPASAN
                     KITAGFTPWQEPWLPK"
     gene            complement(2043..>2458)
                     /locus_tag="VFX21_12910"
     CDS             complement(2043..>2458)
                     /locus_tag="VFX21_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase
                     (decarboxylating)"
                     /protein_id="HEX5096916.1"
                     /translation="IKHANVGMDTHEVDAETTPLRDPWAYQYDIDVAEVAEVWRRGSV
                     VGSWLVDLTAAALASSPELKEFAGRVSDSGEGRWTALAAIDEGVPAPVITASLIERFE
                     SRGNGRFTGQVLSAMRSEFGGHAEKQLAENTDTGK"
BASE COUNT          437 a          803 c          800 g          418 t
ORIGIN      
        1 tcaacgaacc cacaagcgcg atcgacaaca cgcctccgag gttgtcgacc ttctccgtcg
       61 tctcgttgac gtgcgccgga atgaagcgca acgcgagcac caacgccacc gcggcgagcg
      121 gcaacgtcac gatgaacacc gagccccacc agaagtgctc gaggagcgca cccgagacca
      181 acgggccgag ggccgagatg ccgccgccca gcgccgacca gagcgcgatc gacttggtcc
      241 ggcccggacc gctccacaac gcagtaatga gtgcgagcgt cgtcgggtac gccataccgg
      301 cggagaggcc gccgagaatg cgcgcaccaa tgaggacgta ctcattgggc gcgtacgcgg
      361 ctatcaggca cgcgggaatc gacaacgcca caccggcgac acacatcatc ttgcggccgt
      421 agcggtcgcc gagcgcaccg agatacagca ccgaagccgc gagtccgagc gagtagccga
      481 cggcgatgag gttgagtgaa gtctgcgagg cgtcgaacgc cttcccgata tccggaagcg
      541 cgacgttcgc gaccgcgagg ttgaggttcg cgaccgccgc cacgagcacc agcgtgatga
      601 ggacaagcgg cgctcgatcc ggcgcggtcg acggctcttc gatgactact tcggcagcca
      661 tggttcctgc cagggtgtga agccggccgt gatcttgttc gcactcgccg ggccccacga
      721 cccttgcgcg tacatctcca ccggcggcgg gttgtcgatc agcggctgga caatccgcca
      781 ggtctcttcg acgaggtcct cgcgtgtgaa gaggatgacg tcgccgtgca acgcgtcacc
      841 gagcaatcgc tcgtacggct ccggtgcatc gccgagctcc tccgcaaaca caaccgaaag
      901 gtcgacggtc ttggtcgccc tcgttccggg cgctttgacc tgcaccaaca tctgcgaacc
      961 gggattcgga tcgatccgca tcacgagctg gttgggttcc ggattgaagt tcggggcaag
     1021 ggcgagatgg ggcggtcgct tgaacaccac gcgcacctcc gtgttccgca ctgccatcgc
     1081 cttcccggcc cgcaggaaga acggcacccc cgaccatcgc cagttctcga tgttcagccg
     1141 cagcgcgcaa aacgtttcgg tgtcggagtc gggcttcacg cccggaaccg accggtagcc
     1201 ctcgtactgg ccgcgcacgt agtgatcggg ttgcacggca ggaatgccgc ggaacacgtc
     1261 gacgtgctgg ttgcgaaggt ggtcggcgtt cgggctcgcg ggtggttccg ccgcgatgag
     1321 actcacgagc tggaacaagt gattttgcac gacgtcgcgc atcgcgccga cgggatcgta
     1381 gaagctgcct cgatcctcgc aaccgaagtt ttcggccatc gtgatctgca cgcactcgat
     1441 gcggtcgcgg ttccacaggg gttcgacgat cgagttcgcg aaccgcagga acaagatgtc
     1501 gagcgccggt tccttcccga ggaagtggtc gatccgcagg atctgccact cgtgcaggtg
     1561 cttgctcagc tgcgcgttga gttccttcgc cgacgcgagg tcgtgcccga acggcttctc
     1621 gacgatgacg cgcgcgttct cggttgcacc gacgctcgcg aggccgtcga cgaccgtgcc
     1681 gaacagcgac ggcggaatct ccagatagaa gactggatgc ttcttcccct cgagcgcttt
     1741 cccgaccttc acgaacgttt cgtggtcggc gtagttgccg acgatcatcg acaggcgtgc
     1801 cgccagccgg gcgaacaccg tttcgtcgag cgtttcgccg ctcgccacga tgctggtgcg
     1861 ggcgtggtcg cgcaggaagt cgtcggacca gtcgtcgagc gagacgccga tgatcgggca
     1921 gttgagcttg ttggtccgtt cgagccggta caacgcgcgg aacgtcatct tgcgggcgag
     1981 atcgcccgag atgccgaaga cgacgagtgc gtcggccggt gcagtgaccg ggtcgactgt
     2041 tgtcacttgc cggtgtccgt gttctcggcg agttgcttct cggcatgccc gccgaactcc
     2101 gaacgcatcg ccgacagcac ttggccggtg aaccggccat tgccgcgcga ttcgaaccgt
     2161 tcgatgagcg aggccgtgat caccggcgcc ggcacgcctt cgtcgatcgc ggcgagggcg
     2221 gtccagcggc cttcgcccga gtccgagacg cgtcccgcga actccttcaa ctccggcgac
     2281 gatgcgagcg cggctgcggt gaggtcgacg agccacgaac cgacgacgga tccgcgccgc
     2341 cacacttccg cgacttccgc gacgtcgatg tcgtattgat acgcccacgg atcacgcaac
     2401 ggcgtggtct cggcgtcgac ctcgtgggtg tccatgccga cgttggcgtg cttgatga
//
LOCUS       DASUGA010000653         4777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_238652, whole genome shotgun sequence.
ACCESSION   DASUGA010000653 DASUGA010000000
VERSION     DASUGA010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238652"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VFX21_12915"
     CDS             <1..852
                     /locus_tag="VFX21_12915"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5096917.1"
                     /translation="FHKAASYFGITLHKTKVRDDWRADVDAMAARVNANTVLVVGSAP
                     QYPQGVIDDIPAIAALATSVGASMHVDACMGGFVLPFMEMNGDAVPPWDFRVDGVTTI
                     SADLHKLGYAPKGASTIIHRTKELRRYQTFVFDDWLGGLYASPGMQGSRPGLPMATAW
                     AVMHRLGIDGYRELTATTIAAAREMAAGVRAVPGLCVLGEPDAHLLAIAAAPGGAVDV
                     FAIGDVLATRGWVLDRQGPPDSLHATVSAGNAPVMAQFLADLRAAADEVRGARAEDRS
                     TNYATLE"
     gene            1018..1605
                     /locus_tag="VFX21_12920"
     CDS             1018..1605
                     /locus_tag="VFX21_12920"
                     /inference="COORDINATES: protein motif:HMM:NF036523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX5096918.1"
                     /translation="MAARPARRTRATETAARKRRLLDAATQLAAEGGYDAVQMRDVAA
                     RAEVALGTLYRHYSSKDQLLVAAMAQQAATLRERMDQRPARGTTAAERVADVLARASR
                     ALERQPRVTQAMLAAMSSPDTAAIPVKDDINATMRAIIANAVGETPVAQLDDVIAVLG
                     AVWFAELTFWSTGLRNGTSMADNLARAASLLLDDR"
     gene            complement(1648..2439)
                     /locus_tag="VFX21_12925"
     CDS             complement(1648..2439)
                     /locus_tag="VFX21_12925"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5096919.1"
                     /translation="MKLGLALPSFVEDPDIPIAVARAAEAAGLDGVFVYDHVWRDIPP
                     PRRPALECFSLLGAVAAETTTISVGTLVARATLRPAATLAAAFETVHRVSGGRLIAAI
                     GAGDSQSRAENEAFGLPFGTIEDRCEALAHAAQAASGRGFPVWIGGHARHVLDVVALA
                     DGWNGWGGTPADYARFGRRVLDVAPDVMLTWGGLICLDADDDAARTKATARNASPNVL
                     VGGPARLAAQLAAYGAAGAQWAVVAPLDASNAANAHRLGEVAGRL"
     gene            complement(2436..3407)
                     /locus_tag="VFX21_12930"
     CDS             complement(2436..3407)
                     /locus_tag="VFX21_12930"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX5096920.1"
                     /translation="MNIDNVTGRSTGTTLGLDIGGTKILGVVLDAQGTVVREHRVPSP
                     VHDLPLLVKACRSLIEELDPDATRPVGVGAAGLVDMQGELSYAPNLPGVRNAPLQADI
                     AAVTGRRVVADNDANVAALGEVAYGAAIGARFALMVTLGTGIGGGLVIDGRVLRGAHG
                     FAGEIGHVTVERDGPLCACGERGHWEAIASGSALGRMARELIATGSGAGILAAAGGVA
                     DDVVGESVATAAAAGDADAIALLHRYADNVAIGLANLANVLDPECIVIAGGLVQLGAL
                     LFGPLAEAFHNRLEGTEYRPPVPVLPATLGTRAGAIGAAVLARDLLS"
     gene            complement(3535..3726)
                     /locus_tag="VFX21_12935"
     CDS             complement(3535..3726)
                     /locus_tag="VFX21_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096921.1"
                     /translation="MTPSTTPGETPADALEHFWKAAHELLAAARTVLDAADGLVEAQM
                     AKKHEPSEPRLRRIDVQDG"
     gene            complement(3723..>4777)
                     /locus_tag="VFX21_12940"
     CDS             complement(3723..>4777)
                     /locus_tag="VFX21_12940"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HEX5096922.1"
                     /translation="TDPAHSLADAYDVPLGDVPTEIAPRLWGQQLNARVRFEQAWDDV
                     RAYMVDILDWAGADTVEAEELAVIPGLDEVFALADIKTFATSGDYDVIVVDCAPTAET
                     IRLLSLPDILSWYMERVFETQRRLTRIARPLLDRVTNLPIAGENVFGAVRTFYDRLDG
                     VRELLTAGDITSARLVVNPERMVVAEARRTFTYLSLFGYHVDAVIANRMLPDAIQDPW
                     FARWKATQTAHLELITDAFAPLPVLAAELAGNELAGHDELSQFGGALYGSLDPTARLA
                     DLEPLRVEAVGDTLVLSLHLPFTHSNEVELGRTEQELFLSVGPHRRALMLPDSLSRRE
                     VAGARVAGDRLEVEFV"
BASE COUNT          718 a         1733 c         1678 g          648 t
ORIGIN      
        1 ttccacaagg cggcgtcgta cttcggcatc accctgcaca agacgaaagt ccgcgacgac
       61 tggcgcgccg acgtcgacgc gatggcagcg cgcgtcaacg cgaacaccgt gctggtcgtc
      121 ggctcggcac cgcagtaccc gcagggcgtc atcgacgaca tccccgcgat cgcggcgctc
      181 gcgacgagcg tcggcgcgtc gatgcacgtc gacgcgtgca tgggcggctt cgtgctcccg
      241 ttcatggaga tgaacggcga cgcagtgcca ccgtgggact tccgcgtcga cggcgtcacc
      301 acgatctccg cggacctgca caagctcggc tacgcgccga agggtgcgtc gacgatcatc
      361 caccgcacca aagagttgcg ccggtaccag acgttcgtgt tcgacgactg gctcggcggc
      421 ctctacgcct cgccgggtat gcagggcagc cggccgggtc tgccgatggc gaccgcgtgg
      481 gccgtcatgc accgcctcgg catcgacggc taccgggagc tcaccgcgac gaccatcgcc
      541 gccgcgcgcg agatggccgc cggcgtgcgc gccgtccccg gactctgcgt gctcggcgaa
      601 cccgacgcgc acctgctcgc gatcgcggcg gcacccggcg gcgcggtgga cgtgttcgcg
      661 atcggcgacg tcctcgccac gcgcggctgg gtgctcgacc gccagggacc gccggactcg
      721 ctgcacgcga cggtgagcgc cggcaacgcg ccggtgatgg cgcagttcct cgccgacctg
      781 cgggccgccg ccgacgaggt ccggggcgcc cgcgccgagg accgcagcac caactacgcg
      841 actctggagt agcccgtcgt acggcgcggt gccggagctc gcggaggcac tcgcggagcg
      901 gagcgcgggc caacaatccc cgctgcctca cgcgagcacg tgcggagcga caaggagacg
      961 tcgagtaagg aacctgcaac aggttctact tctgttctac ggttgttgtt cgtgactatg
     1021 gccgcccgcc ccgcgcgccg cacccgcgcc accgagactg ccgcgcgcaa gcgtcggctg
     1081 ctggacgccg ccacgcaact cgccgccgaa ggcgggtacg acgcggtgca gatgcgcgac
     1141 gtcgcggccc gcgccgaggt ggcactgggc acgctctacc gccactactc ctcgaaggac
     1201 caactgctcg tcgcggcgat ggcccagcag gccgcgacgc tgcgcgagcg catggaccaa
     1261 cgaccggcgc gcggcaccac ggccgccgaa cgcgtcgccg acgtgctcgc gcgcgcatcg
     1321 cgagcgctcg agcgtcagcc gcgcgtcacg caggccatgc tcgccgcgat gtcgtcaccc
     1381 gacaccgcgg cgatcccggt caaggacgac atcaacgcga ccatgcgggc gatcatcgcc
     1441 aacgcggtcg gtgagacgcc cgttgcgcag ttggacgacg tcatcgccgt gctgggtgcc
     1501 gtttggttcg cggagctcac gttctggagc accgggctgc ggaacggcac gtcgatggcc
     1561 gacaacctcg cgcgcgccgc gtcgctcctc ctcgacgacc gctgagacaa cagttctcgg
     1621 gcgtctcaca agggcgaatt ccgcccctca gagacgcccg gcaacctcgc ccaggcggtg
     1681 cgcgttggcc gcgttcgacg cgtccaacgg tgccaccacg gcccactgtg cgccggcggc
     1741 gccgtacgcg gcgagctgcg ccgcgagccg ggccggacca ccgacgagca cgttcggact
     1801 cgcattgcgg gccgtcgcct tggtccgcgc cgcgtcgtcg tcggcgtcga ggcagatgag
     1861 accgccccac gtgagcatga cgtcgggtgc gacgtcgagg acccggcgac cgaagcgcgc
     1921 gtagtcggcg ggtgtgccgc cccagccgtt ccaaccgtcg gcgagcgcga cgacgtcgag
     1981 cacatgccga gcgtgcccgc cgatccatac cgggaagccg cgacccgatg cggcctgcgc
     2041 cgcgtgcgcg agcgcctcgc agcggtcttc gatcgtcccg aacgggagcc cgaacgcttc
     2101 gttctcggcg cggctctgcg agtcgcccgc accgatggcg gcgatgaggc ggccgccgct
     2161 gacgcggtgc acggtctcga acgcggccgc gagtgtcgcc gcgggccgca gcgtcgcgcg
     2221 cgcgacgagc gtgccgacgg agatcgtcgt cgtctcggcc gcgaccgcgc cgagcagcga
     2281 gaagcactcg agcgcgggcc ggcgcggcgg tggtatgtca cgccacacgt ggtcgtagac
     2341 gaagacgccg tcgagaccag ccgcctcggc cgcgcgcgcg accgcgatcg ggatgtcggg
     2401 atcttcgacg aacgacggca gcgcgaggcc gagcttcacg acaagaggtc gcgagccagc
     2461 acggccgcgc cgatcgcgcc ggcgcgcgtg ccgagcgtgg ccggcagcac cgggaccggc
     2521 ggtcggtact cggtgccttc gagccggttg tggaacgcct cggccagcgg tccgaacaac
     2581 aacgcaccca actgcacgag cccgccggcg atgacgatgc actccgggtc gaggacgttc
     2641 gcgaggttcg cgaggccgat cgcgacgttg tccgcgtacc tgtgtagcag cgcaatcgcg
     2701 tccgcatcac cggcggcagc cgcggtcgcg accgattcgc ccacgacgtc gtcggcgacg
     2761 ccgcccgcgg cggccaggat ccccgcaccg ctcccggtgg cgatcagctc gcgcgccatc
     2821 cggccgagtg cgctcccgga cgcgatcgcc tcccagtggc cgcgctcacc gcaggcgcac
     2881 aacgggccgt cgcgttcgac cgtcacgtga ccgatctcgc cggcgaatcc gtgcgcgccg
     2941 cgcagcacgc ggccgtcgat gacgagcccg ccgccgatgc cggtgcccaa cgtcaccatc
     3001 agtgcgaacc gtgcgccgat cgcggcgccg tacgcgacct cgcccaacgc ggcgacgttc
     3061 gcgtcgttgt cggccacgac ccgccgcccg gtcacggccg caatgtccgc ttgcagcggc
     3121 gcgttgcgca cgcccgggag attcggcgca tacgacaact cgccctgcat gtcgacgagt
     3181 ccggccgcgc cgacaccgac cggccgcgtc gcgtccgggt cgagctcctc gatcaggctg
     3241 cggcacgcct tgacgagcaa cggcaggtcg tgcacgggcg acgggaccct gtgctcccgc
     3301 acaaccgtgc cctgcgcgtc gaggacgacg cccaggatct tggtgccccc gatgtcgagc
     3361 ccgagggtcg tgccagtgct ccgtccggtg acgttgtcga tgttcacggc gagtcagggg
     3421 cgtcgctcgc aaggacgcac gacgaagtca gagtgggcct cttgcaagga ggaggacgcc
     3481 gcgaccggcg tcactggccg acgtgagcgc ggcggacgtt gccggtcgga gcacttagcc
     3541 gtcttggaca tcgatgcggc gcaaccgcgg ttcggacggc tcgtgcttct tcgccatctg
     3601 cgcctcgacg agaccgtcgg ccgcgtcgag caccgtgcgc gccgccgcca gcaattcgtg
     3661 cgccgccttc cagaagtgct cgagcgcgtc ggccggcgtt tcccccggcg tggttgacgg
     3721 tgtcatacga actccacctc gagtcggtca ccggcgacgc gcgcgcctgc gacctcgcgt
     3781 ctcgacagac tgtcgggcag catcaacgcg cgccggtgcg gcccgaccga caagaacagc
     3841 tcttgctccg tgcgacccag ctcgacctcg ttgctgtgcg tgaacggcaa gtgcaacgag
     3901 aggacgagcg tgtcgccgac cgcctcgacc cgcaacggct ccaggtcggc caaccgcgcc
     3961 gtcggatcga gcgagccgta gagcgctccg ccgaactgcg acagctcgtc gtggccggcg
     4021 agctcgttcc ccgcgagctc ggccgcgagc accggcagcg gtgcgaacgc gtcggtgatc
     4081 aactcgaggt gcgcggtctg tgtcgccttc cagcgcgcga accacggatc ctggattgcg
     4141 tcgggcagca tgcggttcgc gatcacggcg tcgacgtggt agccgaacag cgacagatac
     4201 gtgaacgtgc ggcgcgcttc ggcgacgacc atgcgctcgg gattcacgac gagacgcgcg
     4261 ctggtgatgt cgccggcggt gagcaactcg cgcacaccgt cgaggcggtc gtagaacgtg
     4321 cggacggcgc cgaacacgtt ctcgccggcg atcggcaggt tcgtcacccg atccagcaac
     4381 ggccgggcga tgcgcgtcag ccggcgctgc gtctcgaaga cccgctccat gtaccagctg
     4441 aggatgtcgg gcagtgacaa cagccggatc gtctcggccg tcggcgcgca gtcgacgacg
     4501 atgacgtcgt agtcgccgct ggtggcgaac gtcttgatgt cggccagggc gaagacctcg
     4561 tcgaggccgg ggatgaccgc gagctcctcc gcttcgacgg tgtccgcgcc ggcccagtcg
     4621 aggatgtcga ccatgtaggc gcgcacgtcg tcccacgctt gttcgaagcg gacgcgggcg
     4681 ttgagctgct ggccccacag ccgcggcgcg atctcggtgg gaacgtcacc gagggggacg
     4741 tcgtaggcgt cggcgagcga gtgcgcggga tccgtcg
//
LOCUS       DASUGA010000654         2230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_275852, whole genome shotgun sequence.
ACCESSION   DASUGA010000654 DASUGA010000000
VERSION     DASUGA010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275852"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..1615
                     /locus_tag="VFX21_12945"
     CDS             293..1615
                     /locus_tag="VFX21_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5096923.1"
                     /translation="MRRIVIVGGGYAGFYTAWKLEKRLRPDEAELVIIDPRPYMTYQP
                     FLPEVAAGSIEARHAAISLRSHLRRTRIIAGGATMIRHADRTVTVRPADGPDFTVDYD
                     IIVVTAGAVTRTLPIPGADEVAIGMKHVEEAVAIRDRVLTAFDRASVLEPGAERRRLL
                     TFAFVGGGFSGVEGFGELLSLATALLKSYPEIRFSELSFHLVEANDRILPEVTDEPGR
                     WVVESLRSRGAHVHLDARVVSAVDGHVELSNGEVFDANLIVWTAGNAAHPVIAKHTDL
                     PTDERGRLIVRADLRIGTQDEPVPDAWAAGDNAAVPDLASPVPGVLTVPNAQHAVRQG
                     KRLAANIAASLHGELTRDYRHDSLGVVATLGLGRGIFQYKRIVVKGPLAWLVHRGYHV
                     LAIPTWERKIRVLAVWLTAALLGRDIVSLLSVQDPRGAFVAGGVPHAR"
     gene            1662..>2230
                     /locus_tag="VFX21_12950"
     CDS             1662..>2230
                     /locus_tag="VFX21_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX5096924.1"
                     /translation="MLNTTVFGHRIADVRGDLRRRTVPNHWTNLFGVVTFALLTIVTV
                     TGLLLMFFYTPSSDPTTYTGSYALLHGAEVSKAFDSTMRLTFDVPGGLLLRQAHHWSG
                     LLLPASIILQILATFFTGGFRRPRRAMWVLLFLIWIVSLAGGWSGYALPDDMLSGTGL
                     RITEGIALGIPVVGTWLSFLLFGGEFPGR"
BASE COUNT          318 a          771 c          753 g          388 t
ORIGIN      
        1 tggaacacct cgagcgcgtc gtcgaggttc gcatccatac ggtcatcctg tcagtgggtg
       61 gcaagtgttc gccgctgcaa gctctgaccg agtcggggcc gcgggtgtga caacgcggtc
      121 ggatgtcgcg acgcggtcgg atgtcgcgac gcggtcggat gtcgcgacgc ggtcggatgt
      181 cgcgacgcgg tcggatatcg cagcgccgtc gatgttcact cggcacccga tgtcacaaat
      241 cggagcccga atcggtctta acgagtgaag gaaacaattc gaggagctgc agatgcgcag
      301 gatcgtgatc gtcggcgggg gatacgccgg tttctacacg gcgtggaagc tcgagaagcg
      361 gctgaggccc gacgaggccg agctcgtgat catcgaccca cggccgtaca tgacctacca
      421 gcccttcctg cccgaggtgg cagccgggtc gatcgaggcg aggcacgcgg cgatctcgct
      481 gcgttcgcac ctgcgtcgga cccgcatcat cgcgggcggg gcgacgatga tccggcacgc
      541 ggaccgaacg gtgaccgtgc gcccggccga cggtcccgac ttcacggtcg actacgacat
      601 catcgtcgtg acggcaggtg ccgtgacgcg cacccttccg atccccggtg ccgacgaggt
      661 cgcgatcggc atgaagcacg tcgaggaggc cgtcgcgatc cgcgaccgcg tgctcacggc
      721 attcgaccgt gcgtcggtgc tcgagcccgg cgcggagcga cgcaggctcc tcaccttcgc
      781 cttcgtcggc ggcgggttct cgggcgtcga gggcttcggt gaactgctct cgctcgctac
      841 ggcgctgctg aagtcgtacc ccgagatccg gttctcggag ctctcgttcc acctcgtgga
      901 ggcgaacgat cgcatcctcc ccgaggtgac cgacgagccc ggccggtggg tcgtcgagtc
      961 gctccggtcg cggggtgccc acgtgcacct cgacgcgagg gtcgtctcgg ccgtcgacgg
     1021 ccacgtcgag ctctcgaacg gcgaggtgtt cgacgcgaac ctgatcgtct ggacggccgg
     1081 caacgccgca cacccggtca tcgcgaagca cacggatctg cccaccgacg agcgcggtcg
     1141 gctcatcgtg cgcgcagacc tccgtatcgg tacccaggac gagcccgtgc ccgacgcatg
     1201 ggcggcgggt gacaacgccg ccgtgccaga tctcgcgtcg cccgttcccg gcgtgctcac
     1261 ggtgccgaac gcgcagcacg cggtgcggca gggcaagcgg ctggcggcga acatcgccgc
     1321 gagcctccat ggtgagctca ctcgcgacta ccggcacgac tcgctcggcg tcgtcgcgac
     1381 cctcgggctc gggcgaggca tcttccagta caagcgcatc gtcgtgaagg ggccgctcgc
     1441 gtggctcgtg catcgcggct accacgtgct cgcgatccct acgtgggagc gcaagatccg
     1501 cgtccttgcc gtgtggttga cggccgcgct cctggggcgc gacatcgtct cgctcctgtc
     1561 ggtgcaggat ccgcgtggcg cgttcgtcgc ggggggtgtt ccccatgccc gttgaccgac
     1621 gatcccctcg agattcgatc agcgacaggt tcgccgctcg ggtgctgaac acgacggtgt
     1681 tcggccaccg catcgccgac gtccgcggcg acctgcgtcg gcgcaccgtc ccgaaccact
     1741 ggacgaacct gttcggcgtc gtgaccttcg cgctcctcac catcgtgacc gtcacgggcc
     1801 tcctgctgat gttcttctac acgccatcga gcgatccgac gacgtatacc ggcagctatg
     1861 ccctgctcca cggcgccgag gtctccaagg cgttcgactc gacgatgcgc ctcaccttcg
     1921 acgttcccgg cggactgctg cttcgccagg cgcaccactg gtcgggcctg ctgttgccgg
     1981 cgtcgatcat cctgcagatc ctggcgacgt tcttcacggg tggcttccgg cgtccgcgcc
     2041 gggccatgtg ggtgctgctg ttcctcatct ggatcgtgag cctcgcgggc ggatggagcg
     2101 gctacgcgct ccccgacgac atgctctcgg gcacgggcct gcgcatcacc gagggcatcg
     2161 cgctcggcat cccggtcgtg ggcacctggc tctcgttcct cctcttcggc ggcgagttcc
     2221 ccggccggat
//
LOCUS       DASUGA010000655         2431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1731505, whole genome shotgun sequence.
ACCESSION   DASUGA010000655 DASUGA010000000
VERSION     DASUGA010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731505"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2108)
                     /locus_tag="VFX21_12955"
     CDS             complement(<1..2108)
                     /locus_tag="VFX21_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011603345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX5096925.1"
                     /translation="MYAELHCHSNFSFLDGASHPEELAEEAARLGLAALAITDHDGFY
                     GIVRFAEAARPLHLPTVFGAEMTLGARKPPNGVPDPPGEHLVVLAEGIAGYARLAKAI
                     SDAQLAGEKGAPRTSIEQLAETARSKVHGHPATTNNDTWIVTTGCRKGTVPAALMDDG
                     PAAAERALHELVIAFGRDRVLVELWDHGDPMDRHRNDALAKLAMRAGVDVIATNNVHY
                     ATPARRPLADALAAIRARRSLDEIDGWLPAAPFAHLRSPAEQQRRFARYPGAVERTVD
                     IAKACAFDLKLAAPNLPDYPVPFGHDEMSWLRELVRRGANVFYPSSHPQYAQAMRQIN
                     YELNVIEQLGFPGYFLLLHDIVEFCRTSDIYCQGRGSAANSAVCYALGVTKADAVALG
                     LLFERFLSPERDGPPDIDLDIEHQRREEVIQYVYERYGRDRAAQVANVITYRPRSALR
                     EMAKAAGVAPGQADALGKWVDRWAAGPEAFNELAPEWESSHQARVHVRTNRQQLRFER
                     TMRDRTRDGVPIAGPDATTRPPPIPPLALELAQEVLDFPRHLGIHSGGMVMADRPLIE
                     FCPVEWARMEGRSVLQWDKDDCAAAGLVKFDLLGLGMLTMLHLAVDMVRSQGVEIDLA
                     TIPQEKEVYDLLCAADTVGVFQVESRAQMATLPRLRPRTFYDLVVEVALIRPGPIQGG
                     SVHPYLRRRNGEEPVTYIHPL"
BASE COUNT          405 a          794 c          820 g          412 t
ORIGIN      
        1 aggggatgga tatacgtgac gggttcttcg ccgttgcggc gccgcagata cgggtgcact
       61 gacccacctt gaatcgggcc gggccggatc aacgccactt cgacgacgag gtcgtagaac
      121 gtgcgcggcc gcaagcgcgg cagcgttgcc atctgggcgc gactctcgac ctggaagacg
      181 ccaacggtgt cggccgcgca caacaggtcg tagacctctt tctcctgcgg aatcgtcgca
      241 agatcgatct caactccttg actgcgcacc atgtcgactg cgagatgcag catcgtgagc
      301 atgccgaggc cgaggaggtc gaacttcacg agcccggccg ccgcacagtc gtccttgtcc
      361 cattgcagga ccgaacggcc ctccatacgc gcccactcga ccggacagaa ctcgatcaac
      421 ggccggtcgg ccatcaccat gccgccggaa tggatgccca ggtggcgcgg gaagtcgagc
      481 acctcctgcg ccagctccaa cgcgagcggc ggaatcggcg gcggccgggt ggttgcatcg
      541 ggcccggcaa tcggcacacc gtcgcgtgtg cggtcgcgca tggtgcgttc gaaacgaagt
      601 tgctgccggt tcgtgcgcac gtggacgcga gcctggtgtg acgactccca ttccggcgcg
      661 agctcgttga acgcttcggg acccgccgcc caacggtcga cccacttgcc gagcgcgtcg
      721 gcttgcccgg gtgcaacgcc tgcagccttc gccatctcgc gcagtgcact gcggggccgg
      781 tacgtgatga cgttcgccac ctgcgcggcg cggtcgcggc cgtagcgctc gtagacgtac
      841 tggatgactt cttcgcggcg ctggtgctcg atatcgaggt cgatatcggg cgggccatcg
      901 cgttcgggcg acaagaatcg ttcgaacaac aagcccagcg cgactgcatc agccttcgtg
      961 acgccgagcg cgtaacacac ggcgctgttc gccgcgctcc cccgtccttg gcaatagatg
     1021 tcggaggtgc ggcagaactc cacgatgtcg tgcagcagga ggaaatagcc gggaaagccg
     1081 agctgctcga tgacgttcaa ctcgtaattg atctggcgca tcgcctgcgc atactgcgga
     1141 tggctcgacg gatagaaaac gttggcgccg cgccgcacga gttctcgcaa ccacgacatc
     1201 tcgtcgtgcc cgaacggcac cgggtagtcg ggcagattcg gtgccgccag tttgaggtcg
     1261 aacgcgcatg cctttgcgat gtcgacggtg cgttcgaccg cgccggggta gcgagcgaag
     1321 cggcgttgct gttcggccgg gctgcgcaga tgggcgaacg gcgccgccgg gagccagccg
     1381 tcgatctcgt cgagcgaacg gcgggcgcgg atcgcggcga gcgcgtcggc caatggacgg
     1441 cgagcgggcg tggcgtagtg cacgttgttg gttgcgatga cgtcgacacc ggctcgcatc
     1501 gcgagcttgg cgagcgcgtc gttgcggtga cggtccattg gatcgccgtg atcccacaac
     1561 tcgacgagca cacggtcgcg accgaacgca atcacgagtt cgtgcagcgc gcgttcggcg
     1621 gccgcgggac cgtcgtccat gagggcggcg ggcacggtgc ctttgcggca accggtggta
     1681 acgatccacg tgtcgttgtt cgtcgttgcc ggatggccgt gcaccttgct gcgcgccgtc
     1741 tcggcgagct gttcgatgct cgtacggggc gcgcccttct cccccgcgag ctgggcgtcg
     1801 ctgatcgctt tcgcaagccg ggcgtagccg gcgatgcctt cggcaaggac gacgagatgt
     1861 tcaccgggcg gatcgggcac accgttcggc ggcttgcgcg ccccgagcgt catctccgcg
     1921 ccgaacacgg tggggagatg cagtggccgc gccgcctcgg caaagcggac gatcccgtag
     1981 aaaccgtcgt ggtcggtgat cgcgagcgcg gccaacccca gccgggccgc ctcttctgcc
     2041 agctcctcgg ggtgactcgc accgtcgagg aacgagaagt tcgaatggca gtgcaactcc
     2101 gcgtacaccg gccgctccca ctccgtcctg ggggaacgac gctatacgaa cgcctgttcg
     2161 ccgaacaagt gtccgtccgt ttgggagcga ttgggttcgc tccacgtccg cacgggaaag
     2221 tttgccctat gccacccccc atgtcggcca gtgcgcgcct cgagaaactc gagcaactga
     2281 cggacaccaa actggcgcgt ctcgacgtcg acgaactcct ccgggagtta ctcctgcgcg
     2341 ttcgagacat ccttgacgtc gatacggccg ccgtcttgac gctggaggag gcgtccgacc
     2401 agctggaagc ccgagccgcg tccggcatcg a
//
LOCUS       DASUGA010000656         3493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_897229, whole genome shotgun sequence.
ACCESSION   DASUGA010000656 DASUGA010000000
VERSION     DASUGA010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897229"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="VFX21_12960"
     CDS             complement(<1..1174)
                     /locus_tag="VFX21_12960"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5096926.1"
                     /translation="MTITSIAEIIRTYGESRPDAPALEYEGTTTSYGELHRRSSQLAN
                     ALAEVGIKGGDRVAFLDKNCPEYFEVTFALAKLNATNVAVNWRLAPAEMAQVINDAGA
                     RALVVGSEFVAHVEKIEGEIPTVKTIVAIGGHPRWIDYAEFRDSGAPDDPGVQSRGDD
                     VAFQLYTSGTTGLPKGVMLTNDNFFKGAMQVTEAWRFTPDSVNLAVMPMFHIAGTGWS
                     MVGMLHGCQTVLLRDVDPARILELIEEFQVTNVFIVPAVIQFLLITPGVEDVDFAPLR
                     AIVYGASPITDTTLVRAMEVFGCEFIQVYGLTETTGAITQLDGVDHDPVNRPGLLRSC
                     GKPYPWVELRIVDPDSGLDVPVGDVGEILIRGPQVMKGYWNNDAATAEAVDTDGWFR"
     gene            complement(1171..1986)
                     /locus_tag="VFX21_12965"
     CDS             complement(1171..1986)
                     /locus_tag="VFX21_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5096927.1"
                     /translation="MPERPAAPAYDDFPHLRFDRPADGILRITLDGPGLNAVDADVHR
                     ELADVWLTVDRDPSTNVALLQGAGKAFSAGGSFDLVESILDDFDARMRVAREARDLVF
                     NVINCSKPIVSAMHGPAVGAGLVAGLLADVSVVSRTARIIDGHTRLGVAAGDHAAICW
                     PLLCGMAKAKYYLLTCDTLTGEEAERIGLVSLCVDDDAVHATALDVATKLNDGAQSAI
                     RLTKHTLNHWYRAQAAVFDASLAYEFLGFTGPEAREGLASHKEKRAPNFKGDR"
     gene            2020..2997
                     /locus_tag="VFX21_12970"
     CDS             2020..2997
                     /locus_tag="VFX21_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020551211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HEX5096928.1"
                     /translation="MIVDCALYEGGLRQTGDLSLAEAARSCRRDGAFVWLGLYEPSAE
                     EFDKVRHEFALHELAAEDAIKAHQRPKIEVYGESLFVVLKSARYIDETESVEFGEIQV
                     FVGKDFAIVVRHGEASSLGETRKRMEARPDLLAHGPSAVLYGVMDRVVDDYAPVALGL
                     DDDIREVELQVFAEGNDTGNPVERIYKLKREVLDMLQATSPLLEPLQLLANGDVPWIV
                     PESHAYFRDVHDHLRRVKDRIDTFSQLLTSVLEANLTRVSVRQNEDMRKISAWVAIAA
                     VPTMIAGIYGMNFEHMPELGSAFGYPAVLGFIFVVCFLLYRAFRRSGWL"
     gene            3098..>3493
                     /locus_tag="VFX21_12975"
     CDS             3098..>3493
                     /locus_tag="VFX21_12975"
                     /inference="COORDINATES: protein motif:HMM:NF014998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX5096929.1"
                     /translation="MSDSDALMWAIEKDPLLRSTITAVSVLEHSPDRDRFMETIERAT
                     RLVPRLRQRVVSSPLQIAPPEYVVDPNFDLGYHVRFTGAPGSGSLRDLLDFASPLAMQ
                     GFDRARPLWEFHVVEGLEGGRAAMIQKLHH"
BASE COUNT          545 a         1148 c         1220 g          580 t
ORIGIN      
        1 agcggaacca cccgtccgtg tcgacggcct cggcggtggc ggcgtcgttg ttccagtagc
       61 ccttcatgac ctgcggcccg cggatcaaga tctcgccgac gtcgccgacc gggacgtcga
      121 gcccactgtc ggggtcgacg atgcgcagct ccacccacgg gtacggcttg ccgcacgagc
      181 gcagcaggcc ggggcggttg acggggtcgt ggtcgacgcc gtcgagctgg gtgatggcgc
      241 cggtggtctc ggtgaggccg tacacctgga tgaactcgca gccgaacact tccatcgccc
      301 tgaccagtgt cgtgtcggtg atcggtgacg cgccgtagac gatggcgcgc agcggcgcga
      361 agtcgacgtc ttcgacgccg ggcgtgatga ggaggaactg gatgaccgcg ggcacgatga
      421 agacgttggt gacctggaac tcttcgatga gctccaggat gcgcgcgggg tcgacgtcac
      481 gcagtagcac ggtctggcaa ccgtgcaaca tgccgaccat cgaccagccg gtgcccgcga
      541 tgtggaacat cggcatcact gcgaggttca ccgagtcggg ggtgaaccgc cacgcttcgg
      601 tgacttgcat cgcgcccttg aagaagttgt cgttcgtcag catcacgccc ttgggcaatc
      661 cggtcgtccc cgacgtgtac agctggaacg cgacgtcgtc gccgcgcgat tgcacgccgg
      721 gatcgtcggg cgcgccgctg tcgcggaact cggcgtagtc gatccaccgc ggatggccgc
      781 cgatcgcaac gatcgtcttc accgtcggga tctcgccctc gatcttctcg acgtgcgcga
      841 cgaactccga gccgacgacg agcgcgcgag cgccggcgtc gttgatcact tgcgccatct
      901 cggccggcgc gagccgccag ttcaccgcga cgttcgtcgc gttcagcttc gcgagcgcga
      961 acgtcacctc gaagtattcg gggcagttct tgtcgaggaa cgcgacgcgg tcgccgccct
     1021 tgatgccgac ctcggcgagc gcgttcgcga gctgcgacga gcgccgatgc agctcgccgt
     1081 acgaggtcgt ggtgccctcg tactcgagcg cgggcgcgtc cggccgcgac tcgccgtacg
     1141 tgcggatgat ctcggcgatc gaggtgatcg tcatcggtcc cccttgaagt tcggtgctcg
     1201 cttctccttg tgcgacgcca acccctcgcg tgcctcgggg ccggtgaacc cgaggaactc
     1261 gtaggccaac gatgcgtcga acaccgccgc ttgggcgcga taccagtggt tcaacgtgtg
     1321 cttcgtcaac cggatcgcgg actgggcgcc gtcgttcagc ttggttgcga cgtcgagtgc
     1381 ggtcgcatgc accgcgtcgt cgtcgacgca caacgacacg aggccgatgc gctcggcctc
     1441 ctcgccggtg agtgtgtcgc acgtcaggag gtagtacttg gccttcgcca tgccgcacag
     1501 cagcggccag cagatcgcgg catggtcgcc ggccgcgacg ccgagccgcg tgtgcccgtc
     1561 gatgatgcgc gcggtgcggc tcacgaccga cacgtcggcc aggagcccgg cgacgagccc
     1621 ggcgccgacg gccggaccgt gcatcgccga gacgatcggc ttcgagcagt tgatgacgtt
     1681 gaacacgagg tcccgggctt cgcgcgcgac gcgcatgcgg gcgtcgaagt cgtcgaggat
     1741 cgactcgacc aggtcgaagc tgccgcccgc ggagaacgcc ttccccgcgc cttggagcag
     1801 cgcgacgttg gtagaggggt cgcggtcgac ggtgagccag acgtcggcca gttcgcgatg
     1861 cacgtccgcg tcgaccgcgt tgagtcccgg gccgtcgagc gtgatccgca agatgccgtc
     1921 ggcgggacgg tcgaaacgga gatgcgggaa gtcgtcgtac gcaggggcag cggggcgctc
     1981 gggcacgggc gtgagtctcg cgcgtgcgag aattgtgggg tgatcgtcga ctgcgcgttg
     2041 tacgagggcg gcctccgcca gacgggcgac ctcagcctgg cggaagcggc gcggtcgtgc
     2101 cgccgcgacg gcgcgttcgt ctggctcggc ctctacgagc cgtcggccga ggagttcgac
     2161 aaggtgcgcc acgagttcgc gctccacgag ctggcggccg aggacgcgat caaggcccat
     2221 caacgcccga agatcgaggt gtacggcgag tcgctgttcg tcgtgctgaa gagcgcgcgc
     2281 tacatcgacg aaaccgagtc cgtcgagttc ggtgagatcc aggtgttcgt cggcaaggac
     2341 ttcgcgatcg tcgtgcggca cggcgaggcg agctcgctcg gcgagacccg caaacgcatg
     2401 gaagcgcggc ccgacctgct cgcgcacggt ccctcggcgg tcctctacgg cgtcatggac
     2461 agagtcgtcg acgactacgc gccggtcgcg ctcgggctcg acgacgacat ccgcgaggtc
     2521 gagctgcaag tgttcgcgga gggcaacgac accggcaacc cggtcgagcg catctacaag
     2581 ctcaagcgcg aagtactcga catgttgcag gcgacctcgc cgctcctcga accgctccag
     2641 ttgctcgcga acggtgacgt gccgtggatc gtgcccgagt cgcacgcgta cttccgcgac
     2701 gtgcacgacc acctacggcg agtgaaggac cggatcgaca cgttcagcca gctgctgacg
     2761 agcgtgctcg aagcgaacct gacgcgcgtc agcgtccgcc agaacgaaga catgcgcaag
     2821 atctccgcat gggtcgcgat tgccgcggtc ccgacgatga tcgcgggcat ctacggcatg
     2881 aacttcgaac acatgccgga gctcggatcg gcgttcggct acccggctgt cctcgggttc
     2941 atcttcgtcg tgtgcttcct gctctaccgc gcgttccgcc gcagcggctg gctctgagcg
     3001 cctgtggctc gcgccccgat tgcgcgagat gatggggccg agggttgggc aatcccggga
     3061 ggcggccatg ccggaaccga tccgcttcga tcgccggatg agcgactcgg acgcgctgat
     3121 gtgggccatc gagaaggacc cgctgctgcg ctccacgatc acggccgtgt ccgtcctcga
     3181 gcattcgccc gaccgcgatc ggttcatgga gacgatcgag cgcgcaacgc gtctcgtacc
     3241 ccggctgcgc cagcgcgtcg tgtcgtcgcc gttgcagatc gcgccgccgg agtacgtcgt
     3301 cgacccgaac ttcgatctcg gctaccacgt ccggttcacg ggcgcgcccg gatcggggtc
     3361 gttgcgagac ctgctcgact tcgcgtcgcc gctcgcgatg caggggttcg atcgcgcccg
     3421 gccgttgtgg gagttccacg tcgtcgaggg cttggagggc ggccgggcgg cgatgatcca
     3481 gaagttgcac cac
//
LOCUS       DASUGA010000657         3454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1036432, whole genome shotgun sequence.
ACCESSION   DASUGA010000657 DASUGA010000000
VERSION     DASUGA010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036432"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFX21_12980"
     CDS             complement(<1..191)
                     /locus_tag="VFX21_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096930.1"
                     /translation="MDEERDEDNATPQATPPTWPLTFDPTTADAAVAPSVTNEAAAHA
                     ESPTEPSAPVAPEPGPPPGP"
     gene            379..744
                     /locus_tag="VFX21_12985"
     CDS             379..744
                     /locus_tag="VFX21_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX5096931.1"
                     /translation="MNLGNSTLVGSVERDMTGYQRREEQETPDEWAWMHHGLCRGANP
                     SEFFPSDGLGVEAAQRICVSCPVRTECLEYALAHRIEHGVWGGASERERRRILRRRRD
                     LAAGIPPAPMTMQHEPQPH"
     gene            complement(776..1774)
                     /locus_tag="VFX21_12990"
     CDS             complement(776..1774)
                     /locus_tag="VFX21_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096932.1"
                     /translation="MKLRSGVVVGATVGVILLVSALIAVGAESDEDAREWDRMVNDAR
                     ESMDTRPFEGTIEITWRDDNGEEQTATIDVRHAHGVLEVEAERTLIATDATRVLGTGN
                     AWSTLARDTAGANLEMAQGKYEVVQEPGSDVAGRTTTMYEARLHGAPVERVYIDDASG
                     IALRRETLDENGDIARSLAFTDIELGSPDTTPSTVQASAGPAAIDHLELPYHDPGEVG
                     DGFRLAGVWERPGDVVQLYYSDGLLSVSVFEQKGHLDDDELPAGGTAVTFDEHDGYRY
                     ETPVGEVLVFERNGVVYTCIGDAPVDEITAIALDVSAPTQSRWERMADVVVSPFGW"
     gene            complement(1771..2238)
                     /locus_tag="VFX21_12995"
     CDS             complement(1771..2238)
                     /locus_tag="VFX21_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015120547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX5096933.1"
                     /translation="MTESNGMDGMDRDMLSAYLDGECTAAERAAIEARLASDPEWQAE
                     LDETAAARDAVRALPMREVPAGFFEAMTIEDSDDADDAPHRPHTWARRTLSIVAVAAA
                     IGFAFMIATPSHSGPTVTPAIAAMSDAHGATASLENDPAVGIAPVAVPVSLAP"
     gene            complement(2235..2726)
                     /locus_tag="VFX21_13000"
     CDS             complement(2235..2726)
                     /locus_tag="VFX21_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5096934.1"
                     /translation="MPDGVPTWEEIARDQGRFLYNVAYRLAGNDADAQDLVQEALVRV
                     RRGLESYTPGSLEGWLARIVTNVFLDEVRRKKRRPVVALPEQPDLVLPPSAAADEASE
                     DLSADVQGALSSLPDDFRTAVVLCDVVGMSYEEIAAAQRIPVGTVRSRIHRGRRLLRS
                     ALS"
     gene            complement(2811..2987)
                     /gene="tatA"
                     /locus_tag="VFX21_13005"
     CDS             complement(2811..2987)
                     /gene="tatA"
                     /locus_tag="VFX21_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX5096935.1"
                     /translation="MGAPELLIVLIIAILVFGGSQVPKLARSLGQAQKEFKSGLEEGA
                     KPASTPKDEDSPSA"
     gene            complement(3042..>3454)
                     /locus_tag="VFX21_13010"
     CDS             complement(3042..>3454)
                     /locus_tag="VFX21_13010"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5096936.1"
                     /translation="DLVARAADGWNTCWAWTPDAYRDRLAVLDAACARHDRDPASVWR
                     TLGLYTLVGENDADLRARFARLQHSSPPGIKTLSDFDTFREGRLVGTVEQVRDQIGQW
                     ESLGVDTIVCGFGAVPFQVGPVDELEMLAEAARP"
BASE COUNT          509 a         1179 c         1201 g          565 t
ORIGIN      
        1 ggtcccggcg gcgggccggg ctccggcgcg accggcgcgc tcggctccgt gggcgattcc
       61 gcgtgcgccg ccgcctcgtt cgtcacgctc ggcgcgacgg ccgcgtccgc agtggtcgga
      121 tcgaacgtca ggggccaggt cggaggcgtc gcctgcggtg ttgcgttgtc ctcgtcccgt
      181 tcctcgtcca tcgggtgctc cagcctcgga aagtgccaaa cggcagtcgt ctgtgttgca
      241 ggaacaaccg ccgagtcctc ccgcagagtt ccctacggga ctgagagttc cctgggaatt
      301 cctatcggca cgcaacgttt ccgagggagt cgagcgtcaa agagtttgtt atacgcccga
      361 tccggggagg tgcagggcat gaacctcggg aactcaacgc tcgtaggctc cgttgaacgc
      421 gatatgacgg ggtaccagcg acgcgaagag caggaaacgc ccgatgaatg ggcatggatg
      481 catcacggcc tgtgccgcgg cgcgaaccct tcggagttct ttccctccga cgggctggga
      541 gtcgaagctg cgcaacgaat ttgcgtgagc tgccccgtgc gcacggagtg cctcgaatac
      601 gcccttgccc atcgcatcga gcatggtgtg tggggcggtg cgtcggagcg ggagcgccgg
      661 cgcattcttc gtcgccgtcg cgacctcgcc gcgggtatcc cacccgcgcc gatgacgatg
      721 cagcacgagc cgcaaccgca ttaacggttg cggttttcgt tttcaccgtt cggtttcacc
      781 aaccgaacgg tgacaccacg acgtcggcca ttcgttccca gcgcgactgc gtgggtgccg
      841 agacgtcgag tgcgatcgcc gtgatctcgt cgactggcgc gtcgccgatg cacgtgtaga
      901 cgacgccgtt gcgttcgaac accaacacct cgccgacggg ggtttcgtag cgatagccgt
      961 cgtgctcgtc gaaggtgacc gcggtgccgc cggccgggag ctcgtcgtca tcgaggtggc
     1021 ccttctgctc gaagaccgac accgacaaca acccgtcgga gtagtagagc tgcacgacgt
     1081 caccggggcg ctcccacacg ccggcgaggc ggaagccgtc gccgacttcg ccggggtcgt
     1141 ggtacggaag ttcgagatgg tcgatcgcgg ccggtcccgc ggacgcttgc accgtcgacg
     1201 gcgtcgtgtc gggcgagccg agctcgatgt cggtgaatgc gagcgaacgc gcgatgtcac
     1261 cgttctcgtc gagcgtctcg cgccgcagcg cgatgccgct cgcgtcgtcg atgtagacgc
     1321 gctcgaccgg tgcgccgtgg agccgtgcct cgtacatggt ggtcgtgcgg ccggccacgt
     1381 cggaccccgg ctcctggacg acctcgtact tgccttgcgc catctcgagg ttcgcacccg
     1441 cggtgtcgcg cgcgagcgtg ctccacgcat tgccggtgcc gagcacccgg gtcgcgtcgg
     1501 tcgcgatcag ggtgcgctcg gcctcgacct cgaggacgcc atgcgcgtgg cggacgtcga
     1561 tggttgcggt ctgctcctca ccgttgtcgt cgcgccacgt gatctcgatc gtgccttcga
     1621 acggccgcgt gtccatgctc tcgcgtgcgt cgttgaccat gcggtcccat tcccgcgcgt
     1681 cttcgtcgct ttcggcaccg acggcgatga gcgcgctgac cagaaggatc acgccgaccg
     1741 ttgcgccgac gacgacaccg ctgcgcagct tcacggggcg agactcacgg gaactgcgac
     1801 cggcgcgatg ccgaccgcgg ggtcgttctc gagcgatgca gtcgcaccgt gcgcgtccga
     1861 catcgcggcg atcgcgggcg tgaccgtcgg gcccgagtgc gacggcgtcg cgatcatgaa
     1921 cgcgaagccg atggccgcgg ccaccgccac gatcgacagc gtgcgccgcg cccatgtgtg
     1981 cggccggtgc ggcgcgtcgt cggcgtcgtc gctgtcttcg atcgtcattg cctcaaaaaa
     2041 accggccggc acctcccgca tcgggagcgc gcgcactgca tcgcgcgcgg cggcggtctc
     2101 gtcgagctcg gcctgccact cggggtcgga tgcgaggcgc gcctcgatcg ccgcccgttc
     2161 ggcggccgtg cactcgccgt cgagatacgc ggagagcatg tcgcggtcca tgccgtccat
     2221 gccgttgctc tccgtcacga gagtgcgctc cgcaagaggc ggcggccgcg gtggatccgc
     2281 gatcgcacgg ttccgacggg aatcctctgc gcggccgcga tctcctcgta cgacatgccg
     2341 acgacgtcgc acagcacgac cgccgtgcgg aaatcgtcgg gcagcgagct gagcgcgccc
     2401 tgcacgtcgg ccgaaaggtc ctcggatgcc tcgtccgcgg ccgcgctcgg cgggagcacg
     2461 agatcgggtt gttccgggag cgcgacgacg ggccggcgct tcttgcgccg cacttcgtcc
     2521 aagaacacgt tcgtcacgat gcgcgccaac catccctcca acgagccggg cgtgtacgac
     2581 tcgaggccgc ggcgcacacg tacgagggcc tcttgcacga ggtcctgcgc gtccgcgtcg
     2641 ttgccggcca ggcggtaggc cacgttgtag aggaaacgac cctggtcgcg agcgatctcc
     2701 tcccaggtgg gaacaccgtc aggcatgcag agaacgttcg ccgggggtgt cgagttcccg
     2761 ggggtgccgg ccgaggtgca gggcgccggc ggtgggcggc gcgccggccg tcaggccgag
     2821 ggggagtcct cgtccttggg cgtcgaggcg ggcttggcgc cttcttcgag gccggacttg
     2881 aactccttct gcgcctgccc gagcgagcgg gcgagcttcg gtacctgcga gccgccgaac
     2941 accaggatcg cgattatcag aacgatgaga agttcgggag cacccatgcg cgtacctcgc
     3001 agtcgcaggt cgacgcgagg ataccaacgc ccggtcgttt ctcacgggcg ggcggcctcg
     3061 gccagcatct cgagctcgtc gaccggtccg acctggaacg gcaccgcacc gaacccgcag
     3121 acgatggtgt cgacgccgag cgactcccat tgaccgattt ggtcgcggac ctgctcgacc
     3181 gtgccgacga gccggccctc ccggaacgtg tcgaaatccg acagcgtctt gatgcccggc
     3241 ggcgaggagt gctgcaggcg ggcgaagcgg gcgcgcagat cggcgtcgtt ctccccgacc
     3301 agcgtgtaca gaccgagcgt ccgccacacc gacgccgggt cgcggtcgtg gcgcgcgcac
     3361 gccgcgtcca acaccgcgag gcggtcgcgg tacgcatcgg gcgtccacgc ccaacacgtg
     3421 ttccaaccgt cggccgcgcg cgcgacgaga tcga
//
LOCUS       DASUGA010000658         3953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_794526, whole genome shotgun sequence.
ACCESSION   DASUGA010000658 DASUGA010000000
VERSION     DASUGA010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794526"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VFX21_13015"
     CDS             <1..309
                     /locus_tag="VFX21_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096937.1"
                     /translation="IGSAPYFVLGSSPKGALRLRIGTPWDWRERYVLRRFDVEPRTAG
                     QPTVDWRAVVREKQTKQEHEVCGHVEVRWSHGRFCGPPEAKVYLDSPHTEVPGYFPLL
                     "
     gene            complement(366..1736)
                     /locus_tag="VFX21_13020"
     CDS             complement(366..1736)
                     /locus_tag="VFX21_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018558833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5096938.1"
                     /translation="MAAARFSIPGFSGELIHPDDASYDEARALFNGMIDRRPALIARC
                     TSNEDVAAAVKFATAENLPMSVYGGGHGVTGAAVVDAGVVVDLRTMHSVTVDPNARVA
                     RVEGGATWGEFDAATAEHGLVSTGGRVSTTGVGGLTLGSGSGWLERKFGFVCDNLVKA
                     EVVTADGRIVTASADENADLFWGLRGGGANFGVVTAFHIRVHELGPIVYGGLLMFPAP
                     AGRDLLRAFRDFMLNAPDEVGAGLAFISAPPEDFVPEPVRGMPVIGVLCCYAGPVEEG
                     EAAYKPLRDLGPALDMVAPMPYVAVQQLLDPSAPKGMQNYWTANFYDSLPDEAVDILV
                     ERATQPVSPLSQVLVVPGGGALSRVSDDETAFGQRNAPWNLHFLSMWPDPADTEKNIK
                     YTRDIANAMDKWSTGRAYLNFIGDEGVGRVEAAFGPEKYKKLQALKTKWDPDNVFRHN
                     QNITPA"
     gene            1819..3078
                     /locus_tag="VFX21_13025"
     CDS             1819..3078
                     /locus_tag="VFX21_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX5096939.1"
                     /translation="MAVTIPVSRYTDGAFAALEHARLWPRVWQFACSLDHVSRPGDFY
                     EHRLGPLSVLVVRGDDGVLRAFQNACRHRGSALCEGAGDDLAEIRCPFHRWTWGLDGR
                     LREVPSRKEFGVLNDDYPLIAARVDTWGPFVFVNLDLDAMPLAEFLEAVPGDCAWARV
                     EEFRCTASVSLTAQCNWKTLIDGFSETYHVQGIHREMLPMCDDVNGPQQIWGHHGKLE
                     QPYGLPSPRLRDKPDDQGVWEAFVEIMGVRVGNAEQRDAGPAPEVAGKPFRAALADTI
                     RTRVRERGIDTLDRLSDDKVLNMQQYNLFPNVTVLVFSDMIQLVRSRPGATHLDAWMD
                     VFAFERIPPSDAERRRPFDYLLPPDAEPPLGLVLGQDVANFERSQRGMHQPGLTHMTV
                     SATEECRIVNLHRNLDRYLGVDATRDH"
     gene            3078..>3953
                     /locus_tag="VFX21_13030"
     CDS             3078..>3953
                     /locus_tag="VFX21_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005086601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX5096940.1"
                     /translation="MDFADSPEQLMLREAVRDVASKFGHDYYAEKSRTGGKADELWKA
                     VGDLGFLGVHIPEAYGGGGGGLSDLYVVCEELAAQGCPLLLILVSPAICGELIAKFGT
                     DAQRERWLRPMAAGEKMVFAITEPDAGSNSHNIATTATRDGDVYRVKGSKTFISGVDE
                     AGAMVVVTRTGIDEDSGRGRLSLFVVDTDAPGLERTHIPVEIAAPEKQFTLFFDNVEV
                     PADRLLGEEGEGLRQVFVGLNPERIMSAAICTGIGRYALERAAAYARDREVWGTPIGR
                     HQGVAHPLAIAKIEVE"
BASE COUNT          644 a         1341 c         1355 g          613 t
ORIGIN      
        1 atcggcagcg cgccgtactt cgtgctcgga tcgtcaccga agggcgcctt gcggctgcgc
       61 atcggcacgc cctgggactg gcgcgagcgc tacgtgctgc gccgcttcga cgtcgagcca
      121 cgcacggccg gccaaccgac cgtcgactgg cgcgcggtcg tgcgcgagaa gcagacgaag
      181 caggaacacg aggtgtgcgg gcacgtcgag gtgcgctgga gccacggccg cttctgcggt
      241 ccacccgagg ccaaggtgta cctcgacagc ccccacaccg aagtccccgg ctacttcccc
      301 ctgctctaac gactcgggcg tcttacaagg gcggaattcg cccctgtaag acgcccgacc
      361 atcgtttacg ccggggtgat gttctggttg tggcggaaga cgttgtccgg gtcccacttc
      421 gtcttcagcg cctggagctt cttgtacttc tcggggccga acgcggcctc gacgcgaccc
      481 acgccttcgt cgccgatgaa gttgaggtag gcgcggccgg tcgaccactt gtccatcgcg
      541 ttcgcgatgt cgcgcgtgta cttgatgttc ttctcggtgt cggccgggtc gggccacatc
      601 gagaggaagt ggaggttcca cggcgcgttg cgctggccga acgcggtctc gtcgtcggac
      661 actcgcgaaa gtgcgccgcc gccgggcacc accagaacct gtgagagcgg tgaaaccggt
      721 tgtgtcgccc gctcgacgag gatgtcgacg gcttcgtcgg gcaacgagtc gtagaagttc
      781 gcggtccagt agttctgcat gcccttaggg gccgacgggt cgagcagctg ttgcacggcg
      841 acgtagggca tcggcgcgac catgtcgagc gcgggcccga ggtcgcgtag cggcttgtat
      901 gcggcttcgc cttcttcgac cggaccggcg taacaacaca ggacgccgat gacgggcatg
      961 ccgcgcaccg gctccgggac gaagtcttcg ggtggcgcac tgatgaacgc gagcccggca
     1021 ccgacctcgt cgggcgcgtt cagcatgaag tcgcggaatg cgcgcaacag gtcgcgaccg
     1081 gccggcgccg ggaacatcaa caggccgccg tagacgatcg ggccgagctc gtgcacgcgg
     1141 atgtggaacg cggtgacgac gccgaagttc gcacctccgc cgcgcagacc ccagaacaag
     1201 tccgcgttct cgtcggcgga cgcggtgacg atgcgcccgt cggcggtgac gacctcggcc
     1261 ttgacgaggt tgtcgcagac gaatccgaac ttgcgctcga gccaaccgga gccgctgccg
     1321 agcgtcaggc cgccgacacc ggtggtcgag acgcgcccac cggtcgacac gagaccgtgc
     1381 tcggccgtcg ccgcatcgaa ctcgccccag gtcgcgccac cttcgacgcg cgcgacgcgc
     1441 gcgttggggt cgacggtcac gctgtgcatg gtgcgcaggt cgacgacgac acccgcatcg
     1501 acgaccgccg cgccggtgac gccgtggccg ccgccgtaca cggacatcgg gagattctcc
     1561 gcggtcgcga acttcaccgc cgcggcgacg tcctcgttgc tcgtacagcg cgcgatgagc
     1621 gccgggcggc ggtcgatcat gccgttgaag agggcacgcg cctcgtcgta gctcgcgtcg
     1681 tcgggatgga tcagctcacc gctgaatccg gggatcgaaa agcgcgcggc agccatgccg
     1741 ttcctccatg tcgagtcatg ggagtttcgc accaccccac cctcccggat caaggggctt
     1801 ccgaagtacc gtggcgccat ggccgtgacg atccccgtgt cgcgctacac cgacggcgcg
     1861 ttcgccgcgc tggagcacgc tcgcctctgg ccgcgggtct ggcagttcgc ctgcagcctg
     1921 gaccacgtca gccgccccgg cgacttctac gagcaccggc tcggcccgct ctcggtactc
     1981 gtcgtccggg gtgacgacgg cgtgctgcgc gcgttccaga acgcgtgccg ccaccggggc
     2041 agcgcactgt gcgaaggcgc gggcgacgac ctggccgaga tccgctgccc gttccaccgc
     2101 tggacgtggg ggctcgacgg gcggctgcgg gaggtgccgt cgcgcaagga gttcggcgtc
     2161 ctcaacgacg actacccgct gatcgcggcg cgcgtcgaca cctggggtcc gttcgtgttc
     2221 gtcaacctcg acctcgacgc gatgccgctc gccgaattcc tcgaggcagt gcccggcgac
     2281 tgcgcgtggg cgcgcgtcga agagttccgc tgcaccgcgt cggtgtcgct caccgcacag
     2341 tgcaactgga agacgctgat cgacgggttc agcgagacgt atcacgtgca aggcatccac
     2401 cgcgagatgc tgccgatgtg cgacgacgta aacggtccgc aacagatctg gggtcatcac
     2461 ggcaagctcg aacagcccta cggactcccc tcgccgcggc tgcgcgacaa gcccgacgat
     2521 caaggcgtgt gggaagcgtt cgtggagatc atgggcgtgc gcgtcggcaa cgcggagcaa
     2581 cgcgatgcgg gcccggcacc ggaggtcgcg ggcaagccgt tccgtgctgc gttggccgac
     2641 accattcgca cacgcgtgcg cgagcgcggc atcgacacgc tcgaccggtt gagcgacgac
     2701 aaggtcctca acatgcagca gtacaacctg ttcccgaacg tcaccgtgct cgtgttctcc
     2761 gacatgatcc agttggtacg ttcgcgaccg ggggcgacgc acctcgacgc gtggatggac
     2821 gtgttcgcgt tcgagcgcat cccgccgagc gacgcggagc gccggcgccc gttcgactac
     2881 ctgctcccac ccgacgcgga accgccgctc gggctcgtgc tcggccagga cgtcgcgaac
     2941 ttcgagcgca gccagcgcgg catgcaccaa ccgggcctga cacacatgac ggtgtcggca
     3001 accgaggagt gccgcatcgt caacctgcac cgcaacctcg atcgctacct cggcgtcgac
     3061 gccacgcgcg accactgatg gacttcgccg acagtcccga acaactcatg ctgcgcgagg
     3121 cggtacgcga cgtcgcgtcg aagttcggcc acgactacta cgcggagaag tcgcgcacgg
     3181 gcggcaaggc cgacgagttg tggaaggccg tcggcgactt gggtttcctc ggcgtgcaca
     3241 tccccgaggc ctacggcggt gggggcggcg gcctgtcgga cctgtacgtc gtctgcgaag
     3301 agctcgccgc gcaaggctgc ccgctgttgc tcatcctcgt gtcgcccgcg atctgcggcg
     3361 agctgatcgc gaagttcggc accgatgcgc agagggagcg gtggctgcgg ccgatggcgg
     3421 ccggcgagaa gatggtgttc gccatcaccg agcccgacgc cggatcgaac agtcacaaca
     3481 tcgcgacgac cgcaacgcgt gacggcgacg tctaccgcgt gaagggatcg aagacgttca
     3541 tctccggcgt cgacgaggcc ggcgcgatgg tcgtggtgac gcgcacgggc atcgacgaag
     3601 acagcggccg cggccggctg tcgttgttcg tcgtcgacac cgacgcgccc ggcttggagc
     3661 gcacgcacat cccggtcgag atcgctgcac ccgagaagca gttcacgttg ttcttcgaca
     3721 acgtcgaggt gccggccgac cgtttgctcg gcgaggaggg cgaggggctg cgccaggtat
     3781 tcgtcggcct caaccccgaa cgcatcatga gcgccgcgat ctgcaccggc atcgggcggt
     3841 acgcgctcga acgcgcggcc gcctacgcgc gtgaccgaga ggtgtggggc acaccgatcg
     3901 gacgtcatca aggcgtcgcg catcccctcg cgatcgcgaa gatcgaagtg gag
//
LOCUS       DASUGA010000659         6148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_915452, whole genome shotgun sequence.
ACCESSION   DASUGA010000659 DASUGA010000000
VERSION     DASUGA010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915452"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VFX21_13035"
     CDS             complement(<1..501)
                     /locus_tag="VFX21_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017947259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5096941.1"
                     /translation="MAATDSIRYEVDGPVAVITIDRFDEVRNAISVPVGVELYRALRR
                     FESDDELSVAVLTGAGGVFSAGFDLKWYARSRRGEVSSPTDAPADAHSMGPTRITLSK
                     PVIAAVEGYAVAGGLELACWCDMRVAAHDAVFGVYCRRWGMPLADGGTVRLTRIVGQA
                     RALDMIL"
     gene            620..1150
                     /locus_tag="VFX21_13040"
     CDS             620..1150
                     /locus_tag="VFX21_13040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX5096942.1"
                     /translation="MDNRSSAPSPPSPFAIATRPPRGRLKRVLRVPVVLQRRGLGWIF
                     GQRILIIEHRGRRTGKQYRTPVEVVAHDGAVASWTVVAAWGGRPEWFANIQANAASAV
                     FVAGHRYDAPAQHLVDHDTAYDILAAYVCAHPIAARLVFRALGWPDPRVPGALRRVSE
                     LVQMVTFVAASPRRST"
     gene            1191..1595
                     /locus_tag="VFX21_13045"
     CDS             1191..1595
                     /locus_tag="VFX21_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5096943.1"
                     /translation="MASVSVRYIVTDVDEAIAFYCDDLGFKEVMHPAPAFAMLTRGDL
                     RLTLTAPGGGPGGGQAMPDGTLPAPGGWNRFALEVEDLDAEVSRLRQRGARFRNDIVT
                     GVGGKQILVEDPSGNPVELFQPLLDEAKMSSS"
     gene            complement(1675..2490)
                     /locus_tag="VFX21_13050"
     CDS             complement(1675..2490)
                     /locus_tag="VFX21_13050"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX5096944.1"
                     /translation="MDLAAARQAYDELGYFVAPPLFDADEVERFRNATARVIAGEYRG
                     PRPPTLCLPVHPSEHDLRKIDNAWWADPDLAALATDPRLAEIAAALLDAPAVRLWQDQ
                     LLYKPPGGPESTTIGWHQDWASWDTVASRGAFVTAWVAFDDVDDRNGAMQMIPGSHQW
                     GLVPGGSNFFATDRDAQLAGFGDRDVEPTSMVMIAGQVSFHHPLTFHGSGANTSDRLR
                     RSLAVHFVDASVTAVAREGIWQHYNLALFTERGGRLGEPYQFDDICPLVYPAA"
     gene            complement(2490..3914)
                     /gene="cysS"
                     /locus_tag="VFX21_13055"
     CDS             complement(2490..3914)
                     /gene="cysS"
                     /locus_tag="VFX21_13055"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX5096945.1"
                     /translation="MRLFNRLTRQVEDFHPLEPGHVRIYTCGPTVYSRPHIGNYRAFV
                     FADLLRRSLEFEGYRVTHVMNITDVGHLTVDDIADAAGVDKLERAASELKVHPLDLAR
                     KYEAMFHEDLATLHCLPATHYPRATDYVPGMIALIERLIDNGHAYVVDGNVYYDVSSF
                     PAYGALSGNTVADLQAGASERVDERHEKRRPEDFALWKHDPEHLLQWDSPWGSGFPGW
                     HIECSTMSMSLLGESFDIHTGGPDNMFPHHECEIAQSEGATGKRFVQFWMYCGYLNFG
                     DRKMSKSSGAVVNVPELVESGFSGADIRYTMLTAHYRTPMAFDETVLVSERGARARLD
                     RFVNVELPSRPEGPSNPEAVAAVEQFRSDFRAAIADDLNTPQAIAALHVLVRSINRLG
                     VNAVDANAALAAVREADTVLAVLEHSEEDELAEQAQALLDRREIARRDRDFATADAIR
                     DELRALGIEIEDTDSGPRWRRITD"
     gene            complement(4040..4474)
                     /locus_tag="VFX21_13060"
     CDS             complement(4040..4474)
                     /locus_tag="VFX21_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096946.1"
                     /translation="MRNPVLAEMDILVGEWNITLSDAWFLEASEQPQHGHATVRWLGE
                     AFIEMTAEMNGEPTWHFVFGRSDANERLLAFYHDPRPASRVFEMTFGGGEWSMRRADP
                     DMHQGFAGTVTGDRIDLRVDASDDAGVTWRKDFDLVFERVRR"
     gene            4556..4720
                     /locus_tag="VFX21_13065"
     CDS             4556..4720
                     /locus_tag="VFX21_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096947.1"
                     /translation="MAVIFFVVVGIAIALALVPFVAGARRKQQAQTPGAARQSHLHVA
                     QPDDDGAQSE"
     gene            4752..5141
                     /locus_tag="VFX21_13070"
     CDS             4752..5141
                     /locus_tag="VFX21_13070"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5096948.1"
                     /translation="MTDDPLGTPFPDFQPTLYALATVVYAERDGEILLLKRAGGALTG
                     QWFMPGGAVDPGERPEAAARRELLEESGLEIDGELELVGAYPIWVYGGDVLQLSYRGR
                     VAAGDVTISHTNTPTNSGSTRSACAPC"
     gene            5126..5254
                     /locus_tag="VFX21_13075"
     CDS             5126..5254
                     /locus_tag="VFX21_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096949.1"
                     /translation="MRALLTDDAIAALAAGNERVVGLVTGVRDDLDRYLRRIGRTI"
     gene            complement(5291..>6148)
                     /locus_tag="VFX21_13080"
     CDS             complement(5291..>6148)
                     /locus_tag="VFX21_13080"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5096950.1"
                     /translation="TVDDVVVEDGTVTGIRGRSARGSQSDDRAPIVIGADGRNSHVAR
                     AVDPDEYNTKPMLQWGYYTYWADLPIDGVEIVVRPDRGWGAFPTNDDLTLVVVGWPYA
                     EASAYKADVEANYRKTLELAPDFAARVRTATRAEKFAGGAVAGYFRKPFGPGWALVGD
                     AGYNKDPITAQGITDAFHDAQRCSDALIEVFEGRRSFDEAMSDWQHVRDEQAMPIYEF
                     TANLATLEPPPPEMQQLLGAVYGNQAAMDDFVSVVAATVSPVEFFDPDHIGGIMAAAN
                     GAKEEVAPK"
BASE COUNT          944 a         2092 c         2101 g         1011 t
ORIGIN      
        1 gaggatcatg tcgagcgcgc gggcctgccc gacgatgcgc gtgagccgta cggtgccgcc
       61 gtctgcgaga ggcatgcccc accgccggca atacacaccg aacacggcgt cgtgcgcggc
      121 gacccgcatg tcgcaccaac acgcgagctc gagcccgccg gcgaccgcgt acccctcgac
      181 cgcggcgatc accggcttgg acagcgtgat ccgtgtcggg cccatgctgt gcgcgtcggc
      241 gggcgcgtct gtcgggctcg agacctcacc gcgccgggag cgcgcgtacc acttgaggtc
      301 gaagccggcc gagaacacac cgccggcgcc ggtgaggacc gccacggaca gttcgtcgtc
      361 ggactcgaac cggcgcaacg cgcggtacag ctcgacgccg accggcacgc tgatcgcgtt
      421 gcggacctcg tcgaagcggt cgatcgtgat gaccgcgacc ggcccgtcga cctcgtagcg
      481 gatgctgtcg gtcgcagcca tgcgctgccc tccttgccgg gctcggatcg tcacgacagt
      541 acgcggccga cggcgacgag cgaagagtga tgtcgcgcgc gtatgtactg gagagcacgg
      601 gacctagcat tcgacgcgga tggacaaccg atcgtccgcg ccctcgccgc cgagcccctt
      661 cgcgatcgcg acgcgtccac cccgtggccg gctgaagcgc gtgctccgcg tgccggtcgt
      721 gctgcagcgt cggggactcg ggtggatctt cggccaacgc atcctgatca tcgagcaccg
      781 aggccggcgc accgggaagc aataccggac accagtggaa gtcgtcgccc acgacggcgc
      841 cgtcgcatcg tggaccgtcg tcgcggcgtg gggcggtcga cccgagtggt tcgcgaacat
      901 ccaggccaac gcggcgtccg ccgtcttcgt tgccgggcac cgttacgacg cgccggcgca
      961 gcatctcgtg gatcacgaca ccgcgtacga catcctggcc gcgtatgtct gcgcccatcc
     1021 gatcgccgcc cggctcgtgt tccgcgcgct cggctggccc gacccgcgtg ttcccggggc
     1081 gttgcgccgt gtcagcgagc tggtgcagat ggtcacgttc gtcgccgcgt ctccgcgccg
     1141 cagcacatga gtggcatgcg tcgggtgaca gaacaagagg aggaagcacc atggcatccg
     1201 tcagcgtccg ctacatcgtc accgacgtcg acgaggccat cgcgttctac tgcgacgacc
     1261 tcggcttcaa ggaggtgatg caccctgcgc cggcattcgc gatgctgact cgcggcgatc
     1321 ttcgattaac gttgaccgcg cccggcggcg gcccgggcgg cggacaggcg atgcccgacg
     1381 gaacgctgcc ggcaccgggc ggctggaacc gattcgcgct cgaagtcgaa gaccttgacg
     1441 ccgaggtatc tcgcttgcga cagcgcggtg cccggttccg caatgacatc gttaccggcg
     1501 tcggtggcaa gcaaattctc gtcgaggacc catcgggcaa ccccgtcgag ctctttcagc
     1561 cgttgctcga cgaagcgaag atgagctcgt cgtaatcccc gccgttggcg ggcgtgttgg
     1621 ttcgacgtgc cgacgctgct gccgaagggc gcccacttcc ttggcgatgt tgcgtcaggc
     1681 ggcgggatag accagcgggc agatgtcgtc gaactggtac ggctccccga gccggccgcc
     1741 gcgttcggtg aacaacgcga ggttgtaatg ctgccagatg ccctcgcgtg cgaccgcggt
     1801 caccgatgcg tcgacgaagt gcaccgcgag cgatcggcgg agacgatcgg acgtgttcgc
     1861 gccgctgccg tggaaggtca acgggtgatg gaagctgacc tggccggcga tcatcaccat
     1921 tgacgtcggc tcgacatcgc gatcaccgaa gccggcgagt tgcgcgtcgc ggtcggtcgc
     1981 gaagaagttg ctgccgccgg gcaccagacc ccactggtga ctgcccggga tcatctgcat
     2041 cgcgccgttg cgatcgtcga cgtcgtcgaa cgcgacccac gcggtcacga acgcgccgcg
     2101 cgacgcgacg gtgtcccagc tcgcccagtc ctggtgccac ccgatggtcg tcgactcggg
     2161 gccgcccggc ggtttgtaca gcagctggtc ctgccagagg cgaacggcgg gcgcgtcgag
     2221 gagcgccgcc gcgatctcgg ccaggcgggg gtcggtcgcg agcgccgcga ggtcggggtc
     2281 ggcccaccac gcgttgtcga tcttgcggag gtcgtgctcg gaagggtgta cgggcaggca
     2341 gagggtgggc ggtcgcggac cgcggtattc gcccgcgatg acgcgcgccg tcgcgttacg
     2401 gaaccgctcg acttcatcgg catcgaacaa cggcggcgcg acgaagtacc cgagctcgtc
     2461 gtacgcctgc cgggccgccg ccagatccac tagtcggtga tccgtcgcca gcgggggccg
     2521 ctgtcggtgt cctcgatctc gatcccgagt gcgcgcaact cgtcgcggat cgcatcggcg
     2581 gtcgcgaagt cgcggtcgcg gcgcgcgatc tcccgacgat cgagcagtgc ctgcgcctgc
     2641 tcggccagct cgtcttcctc ggagtgctcg agcaccgcga gcacggtgtc ggcctcgcgc
     2701 acggcggcca gggctgcgtt cgcatcgacg gcgttgacgc ccaaccggtt gatcgaccgc
     2761 acgagcacat gcagcgcggc gatcgcctgc ggcgtgttca agtcgtcggc aatcgcggcc
     2821 cggaagtcgc tgcggaactg ctcgaccgcg gcgacggctt cgggattcga cggaccctcc
     2881 ggccgtgacg gcagctcgac gttgacgaac cggtcgagac gagcccgcgc gccacgctcg
     2941 ctgaccagca ccgtctcgtc gaaagccatc ggcgtgcggt agtgcgcggt gagcatcgtg
     3001 tagcgaatgt cggcaccgct gaaccccgac tcgaccagct cgggcacgtt cacgacggca
     3061 ccgctgctct tgctcatctt gcggtcgccg aagttcaagt acccgcagta catccagaac
     3121 tgcacgaagc gcttgccggt cgcgccttcg ctctgcgcga tctcgcattc gtggtgaggg
     3181 aacatgttgt cggggccgcc ggtgtggatg tcgaagctct cgcccaacag cgacatcgac
     3241 atcgtcgagc actcgatgtg ccaacccggg aagcccgagc cccacggcga gtcccactgc
     3301 aagaggtgct cggggtcgtg cttccagagc gcgaagtctt ccgggcgccg cttctcgtgc
     3361 cgctcgtcga cgcgttcgga cgcgcccgct tgcaggtcgg cgaccgtgtt cccgctcagc
     3421 gcgccgtacg ccgggaacga cgagacgtcg tagtacacgt tgccgtcgac gacgtacgcg
     3481 tgaccgttgt cgatgaggcg ttcgatcaac gcgatcatgc cggggacgta atcggttgcg
     3541 cgcgggtagt gcgtcgcggg gaggcagtgc agcgtggcga ggtcctcgtg gaacatcgcc
     3601 tcgtacttgc gcgcgagatc gagcggatgc accttgagct cgctcgcggc gcgctccagc
     3661 ttgtcgacgc ccgccgcgtc cgcgatgtcg tcgacggtga gatggccgac atcggtgatg
     3721 ttcatcacgt gcgtgacgcg gtagccctcg aactcgagcg accgccgcag aaggtcggca
     3781 aagacgaacg cgcggtagtt ccctatgtgc ggccggctgt acaccgtcgg gccgcaggtg
     3841 tagatccgga catgacccgg ctccagcgga tggaagtcct cgacttgtcg cgtcaggcgg
     3901 ttgaagagtc gcacgagaaa cggggcttcc aacgctcgct cgcggcgtcg gtcgccgcgc
     3961 cagcgcatcg caaagtgtag ctggcgggcc cgagatgaca gcgggctcct gtgactcgcg
     4021 cggctgactc cggcgggcgt tagcggcgca cgcgctcgaa gacgagatcg aagtccttgc
     4081 gccacgtgac gccggcgtcg tcggacgcgt cgacccgcag gtcgatgcgg tctcccgtga
     4141 ccgttccggc aaagccctga tgcatgtccg gatcggcgcg ccgcatcgac cattcgccac
     4201 caccgaaggt catctcgaac acacgcgacg ccggccgagg gtcgtggtag aacgcgagga
     4261 gtcgctcgtt ggcgtcgctc cggccgaaca cgaagtgcca tgtcggttcg ccgttcatct
     4321 ccgccgtcat ctcgatgaac gcttcaccga gccagcggac ggtcgcatgg ccgtgctgcg
     4381 gctgttccga tgcttccagg aaccacgcgt ccgacagcgt gatgttccac tcaccgacca
     4441 gtatgtccat ctccgcgagt acgggattgc gcacccgggc ctcctctcgg cgtgtacatc
     4501 tcagacgaca gggcgcgacg attctcatcg cgccctcact tcctagactg cccacatggc
     4561 cgtgatcttc ttcgtcgtgg tcgggatcgc gattgccctc gcgctcgttc cgttcgtggc
     4621 gggcgcgcgc cgcaagcagc aggcgcagac tccgggcgca gcgcgccagt cgcatctcca
     4681 cgtggctcag ccggacgacg acggagcgca gagcgagtga gcctccttcg cggccgccgg
     4741 agcgtcgccc gatgaccgac gatccactcg ggacgccgtt tcccgacttt caaccgacgc
     4801 tgtacgcgct ggcgacggtt gtctacgcgg agcgggacgg cgagatcttg ctgttgaagc
     4861 gcgcgggtgg cgcgttgacg gggcagtggt tcatgcccgg tggtgcggtc gatcccggtg
     4921 agcgtcccga agccgcggcc cgccgcgagc tgctcgaaga gagtggtctc gagatcgacg
     4981 gtgagttgga gctcgtcggt gcgtacccga tctgggtgta cggcggcgac gtcctccagc
     5041 tgtcgtaccg cggccgggtc gccgccgggg acgtcaccat cagccacacg aacacaccga
     5101 ccaacagtgg gtcgacccgg tcggcatgcg cgccctgctg accgacgacg cgatcgccgc
     5161 cctcgcggcc gggaacgaac gcgtcgttgg tctcgtcacc ggcgttcggg acgacctcga
     5221 ccggtacctc cgccgcatcg gtcgcacgat ctgaaccgga aacggacggg gcgccggtgc
     5281 tcggcgtgcg tcacttcggt gcgacctctt ccttcgcacc gttggccgcg gccatgatcc
     5341 cgccgatgtg atcggggtcg aagaactcga ccggtgacac ggtcgcggcg acgacgctga
     5401 cgaagtcgtc catggcggcc tggttgccgt acaccgcacc gagcagctgc tgcatctccg
     5461 gcggcggcgg ctccagggtc gccaggttcg cggtgaactc gtatatcggc attgcctgct
     5521 cgtcccgaac gtgttgccag tccgacatgg cctcgtcgaa ggaacgccgt ccttcgaaga
     5581 cctcgatgag ggcgtcgctg catcgctgcg cgtcatggaa cgcgtcggta atgccctgcg
     5641 ccgtgatcgg gtccttgttg tatcccgcat cgccgaccag cgcccagccc ggtccgaacg
     5701 gcttgcggaa gtaaccggcc acggcgccgc ccgcgaactt ctcggcgcgt gttgccgtcc
     5761 gcacccgcgc cgcgaagtcg ggcgcgagct cgagcgtctt ccggtagttg gcttcgacat
     5821 cggccttgta ggcggacgcc tcggcgtagg gccaaccgac gacgacgagg gtgaggtcgt
     5881 cgttggtggg aaacgcgccc caccctcgat ccggccgaac gacgatctcc acaccgtcga
     5941 tggggagatc ggcccagtac gtgtagtagc cccactgcag catcggcttc gtgttgtatt
     6001 cgtcagggtc gaccgcgcgc gccacgtgcg agttccgacc gtcggcgccg atgacgatcg
     6061 gtgcccggtc gtccgactgt gatccgcgcg cactcctgcc gcggatgccg gtcacggtgc
     6121 cgtcctcgac gacgacgtcg tcgacggt
//
LOCUS       DASUGA010000660         3594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_481635, whole genome shotgun sequence.
ACCESSION   DASUGA010000660 DASUGA010000000
VERSION     DASUGA010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481635"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="VFX21_13085"
     CDS             complement(<1..1074)
                     /locus_tag="VFX21_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096951.1"
                     /translation="MRPLRLGLLFAIALFLTLSTAGNARAQTTDDTDGRVDVVQVNGL
                     IDPANAALIRQSIRDAERDNATALVFQMDASGAVDVDMNALYQDFTNAKVPITVWVGP
                     SGGGVRGAAALLARSADYAAVASGASIGAVDPIRYDDTNFKMDVPLKDVKAEAHTLLE
                     FIGSLEQSPLKLSTMKVVELDGKPTRQPKGEVHFHKLTLDAQLAHTLNSPWVAYFLFV
                     VGLALIVFEFFTAGVGIAGAVGAIALLMGCFGFSHLPVQPWAIALLVLGVFGMAVDVQ
                     AGGLGAWTFIGGIALVIGSIFLYGGSSRLDPKWWVLLVVIGGTVVFMLSGMTAMVRSR
                     FSTPTIGREELIGELGQAAADVAP"
     gene            1097..2029
                     /locus_tag="VFX21_13090"
     CDS             1097..2029
                     /locus_tag="VFX21_13090"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEX5096952.1"
                     /translation="MEVADFCSTSRVVIVAGKGGVGKTTVTAALAVTAARAGLSTLIV
                     EVEGKSGLPTMFGAAALGYEELDLEPGIKARFLTPDAALIDYLVTHGMKRISKRLAAS
                     GALEVVATAVPGMKDILVLGKVKSIDESRTADLVIVDAPAAGHAISFLLSARGLLDAV
                     RMGPVRKQAQDVVDLLSDPARCQVMLVTLPEETPVSEVVETAFAIEDRAGVALGPVVV
                     NSVFAPLPEYALSIAADADATHRFVSEREANDLAHAAAFRRERHALQQEQIDRLRARL
                     PLRQIELPFVFTPDVTRPTLDVLADALTSGIEQL"
     gene            2026..3165
                     /locus_tag="VFX21_13095"
     CDS             2026..3165
                     /locus_tag="VFX21_13095"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEX5096953.1"
                     /translation="MKIDELVTDRSVVICCGTGGVGKTTTAAVIALEGARRGRNACVV
                     TIDPAKRLADALGLESLTDQPTEIDPILWGYGDAAPGGRLSALMLDTKSTFDAIVTRD
                     ATSDEQAQRILENRFYRNVSGALGGTQEYMAMEKLHELHDSGEFDLIVVDTPPTRHAL
                     DFLDAPARLTRLLDNRIFRLLMAPTRAGLRVASAAVQAFLKTVARVVGSEVINDIVAF
                     FRAFEGMEDGFRERAQAVGRLLADPDTAFVLITSPRRDALEEATFFAEKLGDSGQTVQ
                     ALIVNRVHPSFGEEAPGGLRAAADALRDDGSEPALRLAALYDNLADFRQVAALERSHL
                     DGLRSTVGDDAVVAFVPYLAHDVYDFGALAEIGGLLFAPDTVPVQ"
     gene            3162..3530
                     /locus_tag="VFX21_13100"
     CDS             3162..3530
                     /locus_tag="VFX21_13100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5096954.1"
                     /translation="MTTILVAADAKWVRDQVRAAFVGPDQNVVEVSRGQDVRTTFAEV
                     NPDLVILDMQIGNMGGVAAAIDLRLEESGGRLDRANILLLLDREADEFIGRRADVDLM
                     LVKPVDAGVLRRAAKQLLPA"
BASE COUNT          603 a         1169 c         1230 g          592 t
ORIGIN      
        1 gggcgcgaca tcggccgctg cttgcccgag ctcaccgatg agttcctcgc gaccaatggt
       61 cggtgtcgag aagcgcgatc gcaccatggc ggtcatgccc gagagcatga acaccaccgt
      121 gccgccgatc acgaccagca acacccacca cttcggatcg agtcgcgacg agccgccgta
      181 caagaagatc gaaccgatga cgagcgcgat gccaccgatg aacgtccacg cgccgaggcc
      241 gccggcttgc acgtcgaccg ccatgccgaa cacaccgagc acgagtaagg caatcgccca
      301 gggttgcacc ggtagatgcg agaacccgaa gcagcccatc agcagcgcga tggcgccgac
      361 cgcaccggcg atgccgacgc ccgcggtgaa gaactcgaag acgatcagcg cgagcccgac
      421 gacgaacaag aagtacgcga cccacggcga gttgagtgtg tgcgcgagct gcgcgtcgag
      481 cgtgagcttg tggaagtgca cctcgccctt cggctggcgc gtcggcttgc cgtcgagttc
      541 cacgaccttc atggtcgaga gcttgagcgg cgactgctcg agcgaaccga tgaactcgag
      601 gagcgtgtgg gcttcggcct tcacgtcctt gagcggcacg tccattttga agttcgtgtc
      661 gtcgtagcgg atgggatcga ccgcgccgat gctcgcgccc gaggcgaccg ccgcgtagtc
      721 agcggaacgc gcgagcagcg ccgccgcgcc gcgcacaccc ccgccactcg gaccgaccca
      781 caccgtgatc ggaaccttgg cgttggtgaa gtcttggtag agcgcgttca tgtcgacgtc
      841 gaccgcgccc gaagcgtcca tttggaagac gagtgcggtt gcattgtcac gttcggcgtc
      901 gcggatcgat tgtcgaatga gtgctgcgtt ggcggggtcg atcaagccgt tgacctgcac
      961 gacgtcgacg cgaccgtcgg tgtcatcggt cgtttgtgcg cgcgcgtttc cggccgtcga
     1021 aagcgtcaag aacagcgcga ttgcgaaaag cagtcccagc cggagcggcc gcacgtctct
     1081 ctcctaccat gaggcgatgg aggtagcgga tttctgctcc acctctcgcg tcgtcatcgt
     1141 cgccggaaaa ggcggcgtcg ggaagaccac ggtaacggct gcccttgccg tcacggccgc
     1201 gcgcgcgggc ttgtcgacgc tcatcgtcga ggtcgaggga aaatccgggc tcccgaccat
     1261 gttcggggcg gccgccctgg gctatgagga gctggacctc gagcccggca tcaaggcccg
     1321 cttcctcacg cccgacgccg ccctcatcga ctacctcgtg acgcacggca tgaagcgcat
     1381 ttcgaagcgc ttggcggcct cgggcgcgct ggaagtggtc gccaccgccg tgccggggat
     1441 gaaggacatc ctcgtgctgg gcaaagtgaa gtcgatcgac gagtcccgca ccgctgacct
     1501 cgtcatcgtc gacgcccccg cggccgggca cgccatctcg ttcttgttgt cggcgcgcgg
     1561 gctgctcgac gccgtccgca tggggccggt gcgcaagcag gcgcaggatg tcgtcgatct
     1621 cctctcggat ccggcgcgct gccaggtgat gctggtgacg ctgcccgagg agacgccggt
     1681 cagcgaagtg gtcgagaccg cgttcgcgat cgaagaccgc gccggcgtcg cgctcgggcc
     1741 ggtcgtcgtg aacagtgtgt tcgcgcccct gcccgaatac gcgctgtcga ttgcggccga
     1801 cgcggatgcg acccatcgct tcgtgtccga acgggaagca aacgatctcg cgcacgcggc
     1861 cgcgttccgg cgagaacggc acgcgttgca gcaggagcaa atcgaccggc tgcgcgcgcg
     1921 attgccgctg cgccagatcg agttgccgtt cgtgttcaca ccggacgtca cgcggccgac
     1981 gctcgatgtc ctggccgacg cgttgacgag cgggatcgag caactgtgaa aatcgacgag
     2041 ctcgtcaccg atcgcagcgt ggtcatctgc tgcggtacgg gcggggtcgg caagaccact
     2101 accgcggcgg tgatcgccct cgaaggcgcg cgccgcggcc gcaacgcatg cgtggtgacg
     2161 atcgacccgg cgaagcggct cgccgacgcg ctcgggctcg agtcgctcac cgaccagccg
     2221 accgaaatcg acccgatctt gtggggttac ggcgatgccg cgcccggcgg ccggctgtcg
     2281 gcgttgatgc tcgacacgaa gtcgacgttc gacgcgatcg ttacgcgcga cgcgacgtcg
     2341 gatgagcaag cgcaacgcat tctggagaac cgcttctacc gcaacgtgtc gggcgcgctg
     2401 ggcgggacgc aggaatacat ggccatggag aagctgcacg agctgcacga ctccggcgag
     2461 ttcgatctca tcgtcgtcga cacgccgcca acgcgacatg cgctcgactt cctcgacgca
     2521 cccgcgcgct tgacgcggtt gctcgacaat cggatcttcc ggttgctcat ggcgccgacc
     2581 cgagccgggc tgcgtgtcgc gagcgcggca gtgcaggcat tcctgaagac cgtggctcgt
     2641 gtcgtcggtt cggaagtgat caacgacatc gttgcgttct tccgtgcgtt tgaagggatg
     2701 gaagacgggt tccgcgaacg cgcccaagcc gtcggccggc tgctggccga tccggatacg
     2761 gcgttcgtgt tgatcacctc gccccggcgc gacgcgctgg aagaggcgac gttcttcgcg
     2821 gagaagctcg gcgactcggg tcagaccgtg caggcgctga tcgtgaaccg ggtacacccg
     2881 tcgttcggcg aggaggctcc cggaggcctg cgcgctgcgg ctgacgcgtt acgcgacgac
     2941 ggctccgagc ccgcgcttcg cctcgcggcg ctgtacgaca acctcgccga tttccggcag
     3001 gtcgcggcgc tcgaacgatc ccacctcgac gggcttcgtt cgacggtcgg cgacgacgcg
     3061 gtggtcgcgt tcgtgcccta tctggctcac gatgtctacg acttcggggc gctcgcggag
     3121 atcggcggtt tgctgttcgc cccggataca gtgcccgttc aatgacgacg atcctcgtgg
     3181 ccgccgatgc gaaatgggtg cgcgatcagg tgcgcgccgc gttcgtgggg cccgatcaga
     3241 acgtggtcga ggtgtcccgg ggccaggatg tgcgaacgac gttcgccgag gtcaatcccg
     3301 atctcgtgat cctcgacatg cagatcggca acatgggcgg cgtggcggcg gcgatcgacc
     3361 tgcggctcga agagtcgggt ggccgtttgg accgggcgaa catcttgttg ctgctcgatc
     3421 gggaggccga cgagttcatc ggccggcgcg ccgacgtcga tctgatgctg gtgaagcctg
     3481 tcgacgcggg cgtgctgcga cgggctgcga agcagctgct ccccgcgtga catgaacgtg
     3541 cggacgcagc gaagccaaat agacgcgcgc gccgaaggcg ctcgatcaag ggcg
//
LOCUS       DASUGA010000661         2092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1417329, whole genome shotgun sequence.
ACCESSION   DASUGA010000661 DASUGA010000000
VERSION     DASUGA010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417329"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /gene="ribD"
                     /locus_tag="VFX21_13105"
     CDS             <1..1135
                     /gene="ribD"
                     /locus_tag="VFX21_13105"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX5096955.1"
                     /translation="HCLRGRARDERMHDGELMQRAIEIARSARRRTAPKPAVGCVIAR
                     EGEIVGIGATEAGLHGAHAEVVALRAAGESARGATAYSTLEPCNHHGATPPCTDALIE
                     AGVARVVVAVGDPDPLVAGAGFARLANAGIEVTTGVETDAAARDLAPYLHHRRTGQAF
                     VTAKVALSLDGRVAAADGSSQWITSPEARADAHELRADAQAIVVGSGTAHADQPKLTV
                     RDVAPMPEHAPLRVLLDARGRTPATGPLFDAALAPTMVFTTAAASSDAKAAWEAAGAK
                     VEVVERGWQGHGVDFDAVFQRLGAEGVLHALVEGGGTLLGALVACENVQRIVAYVAPT
                     LLGTRGLPAFVFNGPETIDAAPRWDLVDTTPVGADVRLTLEPR"
     gene            1135..1737
                     /locus_tag="VFX21_13110"
     CDS             1135..1737
                     /locus_tag="VFX21_13110"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX5096956.1"
                     /translation="MFTGIVEELGRVRAITPNAGGARIEIDATTVLDDVELGASIAVN
                     GCCLTVVAFDEKGWAADAVIETLSLTNLGDLSAGSPVNLERPVRLADRLGGHLVQGHI
                     DGTGTLTARAPQPDDSVFMRFDAPPQVQRYVVHKGSITVDGISLTVAALHETGFTIAV
                     IPHTLAVTTLGTREPGERVNLEADLVAKYVERLLPERSTG"
     gene            1734..>2092
                     /locus_tag="VFX21_13115"
     CDS             1734..>2092
                     /locus_tag="VFX21_13115"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016737433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX5096957.1"
                     /translation="MSPFDNVERAIDAVKRGEFVVVVDDEDRENEGDLIIAAEKMTPD
                     KMAFMIRYTSGVICLPLEGERLDELQLPLMVSGAENTEGQRTAFTVSVDAKAGTTTGI
                     SAADRSATIHALIDPNTV"
BASE COUNT          355 a          729 c          700 g          308 t
ORIGIN      
        1 tcactgcctc cggggtcgag cccgggacga acgcatgcac gacggcgagc tgatgcaacg
       61 agcgatcgag atcgctcgtt ccgcgcgccg acgtaccgcg cccaagccgg ccgtcggctg
      121 cgtgatcgcg cgcgaaggcg aaatcgtcgg cattggcgcg accgaagccg ggttgcacgg
      181 cgcccacgcc gaagtcgtcg cgttgcgcgc ggcgggtgag agcgctcgcg gcgcgacggc
      241 gtattccacg cttgaaccct gcaaccacca cggcgcgaca ccaccgtgca ccgacgcgtt
      301 gatcgaagcc ggcgtcgcgc gggttgttgt cgccgtcggc gatcccgacc cgttggtcgc
      361 cggcgcggga ttcgcgcgcc ttgcgaatgc tggcatcgag gtaacgaccg gtgtcgaaac
      421 cgatgcggcc gcacgcgatc tcgccccgta tctgcatcac cggcgcaccg gccaagcgtt
      481 cgtcacggcc aaggttgcac tcagtctcga cgggcgcgtc gctgcggccg atgggtcgtc
      541 gcaatggatc accagtcccg aagcacgcgc cgacgcgcac gaactccggg ccgacgcgca
      601 ggcgatcgtc gtcggatcgg gaaccgctca cgccgatcag ccgaagctga ccgtgcgtga
      661 cgtcgcgccg atgccggaac atgcgccgct gcgcgtgctc ctcgacgcgc gcggccgcac
      721 gccggcgacc ggcccgttgt ttgatgcggc gctcgcgccg acgatggttt tcacgaccgc
      781 ggccgcgtca tccgacgcca aggcggcatg ggaagcagcg ggcgccaagg tcgaagtggt
      841 cgagcgcggc tggcagggtc acggcgtcga cttcgatgcc gtgttccaac ggctcggggc
      901 cgaaggcgtg ctgcatgcgc tcgtcgaagg cggcggcacc ctcctcggcg cgcttgtcgc
      961 gtgcgaaaac gtgcaacgca tcgtggccta cgtcgcgccg acgctgctcg gcacacgcgg
     1021 cttgcctgcc ttcgtcttca acggccccga gaccatcgac gcggcgcccc gctgggacct
     1081 cgtcgacacg acgccggtcg gtgcggacgt ccgcctcacg ctggagcccc gctgatgttt
     1141 accggcatcg tcgaagaact cggccgggtg cgcgccatca caccgaacgc gggcggcgcg
     1201 cgcatcgaga tcgacgcaac aaccgtgctc gacgacgtcg agctcggtgc atcgattgcg
     1261 gtcaacgggt gctgcctcac ggtcgtcgcc ttcgacgaaa agggctgggc ggccgacgcg
     1321 gtgatcgaga cgctcagcct cacgaacctg ggcgacctca gcgccggcag ccccgtcaat
     1381 ctcgaacggc cggtgcgcct cgccgatcgc ctcggcggcc acctcgtgca gggccacatc
     1441 gacggcaccg gaacgctgac ggcgcgcgcc ccacagcccg atgactcggt gttcatgcgc
     1501 ttcgacgcac ccccgcaggt gcagcgctac gtcgtgcaca agggctcgat caccgttgac
     1561 gggatcagtc tcacggtcgc ggcactgcac gaaacgggat tcacgatcgc ggtgatcccg
     1621 cacacgctcg cggtcacgac gctcggaact cgcgaaccgg gcgagcgcgt caatcttgaa
     1681 gccgatctcg tggccaagta cgtagaacgt ctccttcccg aacggagcac aggatgagtc
     1741 ccttcgacaa cgtcgaacgc gcgattgatg ccgtgaaacg cggcgagttc gtggtcgtcg
     1801 tcgacgacga agaccgcgag aacgagggcg acctcatcat cgcggccgag aaaatgacgc
     1861 ccgacaagat ggcgttcatg atccgctaca cgagtggcgt gatctgcttg ccgctcgagg
     1921 gcgagcgact cgacgagctg caactgccgc tcatggtttc cggtgcggag aacaccgaag
     1981 gccagcgcac ggcgttcacg gtctcggtcg acgcaaaggc agggacgacc actggcatct
     2041 cggccgccga ccgctcggcg acgattcacg cgctcatcga tccgaacacg gt
//
LOCUS       DASUGA010000662         6037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1211442, whole genome shotgun sequence.
ACCESSION   DASUGA010000662 DASUGA010000000
VERSION     DASUGA010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211442"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="VFX21_13120"
     CDS             <1..1139
                     /locus_tag="VFX21_13120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX5096958.1"
                     /translation="PTRLVVHSPDAIRYILTAPGELGFARAYVSGQLTLEGDIFAALA
                     LREHLPEVRLTSRHWIALARLIGATGVRRPPIPREEAHLHGRRHSRARDAAAIAHHYD
                     VGNDFYRIVLGPSMTYSCAVWPSRDATLEAAQATKYDLVCQKLGLRPGMRLLDIGCGW
                     GGMALHAVRHYGVEAVGITLSKEQAAYAMAAARDANLEDRVSFRIQDYRDVRDEQFDA
                     ISSIGMFEHVGLARLHEYFQGVHSLLLPGGRFLNHAISRPPGHRPRFFRYGFIDRYVF
                     PDGELHEIGSVVSAIQADGFEVRHVESLREHYALTLRKWVANLEAQWDDAERIAGSAR
                     ARIWQLYMAASALLFETGRIQVLQTLAVRADGGRSGMPLRPDWH"
     gene            1300..1941
                     /locus_tag="VFX21_13125"
     CDS             1300..1941
                     /locus_tag="VFX21_13125"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEX5096959.1"
                     /translation="MLLHQRSLFGGGEPAVSATPRFERIQLDDESWVDVARGWLEGSD
                     AVFDELVESVDWKQGKRWMYERMVDDPRLSRWFDAQSAVPHPVLADARRALEQHYGVR
                     FGSVGLNYYRDGKDSVAWHNDKELRELDDTLVAILTLGSCRPFLIRPDGGGHSINLAP
                     AGGDLLVMGGRCQMDWEHNVPKAKRAGPRISCSWRWRRASATAAPAPGSADGT"
     gene            complement(1901..2347)
                     /locus_tag="VFX21_13130"
     CDS             complement(1901..2347)
                     /locus_tag="VFX21_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HEX5096960.1"
                     /translation="MTAWHVAQVNIARLLQPVESPKTQEFISLLDPVNALADAAPGFV
                     WRLSATSSMEPTGADPMIVVNLSVWESIEHLEAFVRVPAHAAVMRRRREWFERMAEAY
                     MALWWVPAGTVPTEEDAFERIELIRAQGPTEKAFTFRQRFPAPAPL"
     gene            2391..4487
                     /locus_tag="VFX21_13135"
     CDS             2391..4487
                     /locus_tag="VFX21_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096961.1"
                     /translation="MTVVMAVSFAACDSGDDDDSATSTSAPNGQSTVTDQGFAARQYE
                     YLLSAGAPMDPNSPLSVIASAELARRDQSYTFDASKVGPDAFERIFTRIDEFADTTDF
                     DVLYLLNLWYGYQDELPQATQDAMREHFLKFKYWYTEPTPEGIVDNKYYWSENHRIIF
                     HVDEYLAGLAFPDETFTNDGRTGKEHAEEAQQRILTWLDEKVRFGFTEWHSDVYYQKD
                     VTPLLSLVEFAPDEDLANRAGMMLDVFLLDIATHLQRGTFGVTHGRSYMKDKSIATDE
                     DTFALSKLLFDDTSLPYNEGADAGATLFARAENFALAPVIGAIATSEETTVDQEHMNV
                     PLDPSAPVDPNVVAPYGYDFDDPDNVPFWWERGAQTVWQEVPMTIQTLDEYNLWDSEF
                     FSPFKPLRDAVGSDMAGAQNLAQTLAPQLAFGLLAEVNTYTYRAPNVMLSTAQDYRPG
                     VFSEQIQSWQATLDERALVFTTHPKNEPESGTEWPDSDGYWTGTGSMPRSAQHGTVSV
                     NIYSPTYTPTGPPLDAFGYLDYTHAYFPQEYFDEVVQEGNWTFGRKGDGYVALWSQRP
                     PTWREHDPNVVFTHGLTRPFDLVAMGGAQNVWIAEVGDAAKWETFDAFKAAITAAPVT
                     VDADLNVDYTSPTEGHMRFGSTGPLTVNDTEVDLHPTARIDNPFVTVPFEGKTYEIKA
                     GDDTLTLDFANWKRMQ"
     gene            complement(4450..5556)
                     /locus_tag="VFX21_13140"
     CDS             complement(4450..5556)
                     /locus_tag="VFX21_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014134028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096962.1"
                     /translation="MSITLLTADEATRALALRDLTDPAEGRHAIQRLVDDATRALSRA
                     WNCPVRVYRGTRIVTLADNYDSLGYSPDAVTRDERYTRYVAPDQVLRSHTSALIPGAL
                     RTLGPVDDVLLACPGVVYRRDSIDRLHSGTPHQLDLWRVSRTRELGPGDLKEMIDVLV
                     RTLLPGAEYRVAAREHPYTTHGLQIDVDNDDEWVEIGECGLAGEHVLRGAHVEGTGLA
                     MGLGLDRILMLRKGIADIRLLRDPDPRVAGQLCDLGLYRPVSSHPPVTRDISIAVEDD
                     DTVENMGDRVRHALGADADAVEKVAVLSETPGAELPPIARERLGLVDGQKNVLVRVVL
                     RHPSRTMTRDEANEIRDRVYDALTASVSSLRSRG"
     gene            complement(5751..>6037)
                     /locus_tag="VFX21_13145"
     CDS             complement(5751..>6037)
                     /locus_tag="VFX21_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015287862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5096963.1"
                     /translation="KLLGWETEPVPDTDYTLFKLDGHDIGGAMKPAMPGMPPHWLVYF
                     EVADTDAVVAKAQAAGATVLSPPMDIPAGRFAVLADPQGAVFGVIKSAQQ"
BASE COUNT          991 a         2050 c         2070 g          926 t
ORIGIN      
        1 cgccgacgcg actggtcgtg cactcgccgg acgcgatccg gtacatcctc accgcgcccg
       61 gcgagctcgg cttcgcgcgc gcatacgtct ccggacagct gacgctcgag ggtgacatct
      121 tcgcggcgct cgcattgcgc gagcacttgc ccgaggtgcg gttgacgagc cgccactgga
      181 tcgcgctcgc gcgcttgatc ggtgccaccg gcgtgcggcg accgccgatc cctcgcgagg
      241 aagcgcacct tcacgggcgg cgccactcgc gcgcccgtga cgcggctgcg atcgcgcatc
      301 actacgacgt cggcaacgac ttctaccgca tcgtgctcgg cccttcgatg acctactcgt
      361 gcgccgtgtg gccgtcacgc gacgccacgc tcgaagcggc gcaggccacc aagtacgacc
      421 tggtctgcca gaagctcggt ctgcggcccg gcatgcgact gctcgacatc ggctgcgggt
      481 ggggcggcat ggcgctgcac gcggtgcggc attacggcgt cgaggcggtc gggatcacgt
      541 tgtcgaagga acaggccgcc tacgcgatgg ccgcggcgcg tgacgcgaat ctcgaggatc
      601 gggtgtcctt ccgcatccag gactaccgcg acgtgcggga cgagcagttc gacgcgatca
      661 gttcgatcgg catgttcgag cacgtcggac tcgcccggct gcacgagtac tttcaaggtg
      721 tgcactcgct gctgctgccc ggcggccgct tcctcaacca cgcgatcagc cgcccgcccg
      781 gccatcggcc gcgcttcttc cgctacggct tcatcgaccg gtacgtgttc cccgacggcg
      841 agctgcacga gatcgggagt gtggtgtccg cgatccaggc cgacgggttc gaggtgcgtc
      901 atgtggagag cctgcgcgag cactacgcgt tgacgctgcg caaatgggtc gccaacctcg
      961 aggcgcagtg ggacgacgcc gaacgcatcg cgggctcggc gcgggcacgc atctggcagc
     1021 tgtacatggc cgcgtccgcg ttgctgttcg agaccggccg catccaggtg ctccagacgc
     1081 tcgcggtgcg cgccgacggc ggccgcagcg ggatgccgct ccggcccgac tggcactagc
     1141 gcacaattct ctgtcgcttc aacgtgctcg cgtgaggcag cccggattgt tggcccgccg
     1201 aaagactccg cgagtgcctc cgcaagctcc ggtaccgccc cgtgcgactc gacctgcccg
     1261 ggctttcgct tacgaacaga cgttcgctac cctggcgtga tgctcctcca ccagcggtcg
     1321 ctgttcgggg gcggggaacc ggcggtcagc gcgacgccac gattcgagcg catccagctc
     1381 gacgacgaga gctgggtcga cgtcgcgcgc ggctggctcg aaggttccga cgcggtcttc
     1441 gacgagctcg tcgagtccgt cgactggaag cagggcaagc gctggatgta cgagcgcatg
     1501 gtggacgacc cgcgcttgtc gcgctggttc gacgcgcagt cggcggtgcc gcatcccgtg
     1561 ttggccgatg cgcggcgggc gctcgaacag cattacggcg tgcgcttcgg gagcgtcggt
     1621 ctcaactact accgcgacgg caaggacagc gtcgcctggc acaacgacaa ggagctgcgc
     1681 gagctcgacg acacgctcgt cgcgatcctc acactcggtt cgtgccggcc gttcctcatt
     1741 cgtcccgacg gtggcgggca ctcgatcaac ctcgcgcccg cgggcggcga cttgctcgtg
     1801 atgggtgggc gttgccagat ggactgggag cacaacgtcc cgaaggcgaa gcgcgcgggc
     1861 ccgcgcatct cgtgctcgtg gagatggcgg cgcgcgagcg ctacagcggc gccggcgccg
     1921 ggaagcgctg acggaacgtg aacgccttct cggtcggtcc ctgcgcgcgg atgagctcga
     1981 tgcgttcgaa cgcatcttcc tcggtcggca cggttccggc gggcacccac cacagcgcca
     2041 tgtaggcctc ggccatccgc tcgaaccact cgcggcgacg acgcatcacc gccgcgtgcg
     2101 cgggcacgcg cacgaacgct tcgaggtgct cgatcgactc ccagaccgac aggttcacca
     2161 cgatcatcgg gtcggcgccg gtcggctcca tcgagctcgt cgcgctgagc cgccagacga
     2221 acccgggcgc ggcgtcggcc agtgcgttca caggatcgag cagcgagatg aactcctgcg
     2281 tcttcggcga ctcgactggt tggagaagcc gggcaatgtt gacctgcgcg acgtgccaag
     2341 cagtcatgtg atcggcaagg ctacccggcg tgcgcagatg ggctttggcg atgacggtgg
     2401 taatggcggt gagctttgcg gcgtgtgaca gcggtgacga cgacgacagc gcgacctcga
     2461 cgtcggcgcc gaacgggcag tcgaccgtga cggatcaagg cttcgcggcg cgccagtacg
     2521 agtacctgct gtcggccggc gccccgatgg acccgaacag cccgctgagt gtcatcgcga
     2581 gcgcggagct ggcgcgccgc gaccagagct acacgttcga cgcgagcaag gtcgggcccg
     2641 acgcgttcga gcggatcttc acgcgcatcg acgagttcgc ggacacgacc gacttcgacg
     2701 tcctgtactt gctcaacctc tggtacggct accaagacga actgccgcaa gcgacgcagg
     2761 acgcgatgcg cgagcacttc ttgaagttca agtactggta cacggagccg acgcccgagg
     2821 ggatcgtcga caacaagtac tactggtcgg agaaccaccg gatcatcttc cacgtcgacg
     2881 agtacctcgc cgggctcgcg ttccccgacg agacgttcac gaacgacggc cgcaccggca
     2941 aggagcacgc cgaagaagcg cagcaacgca tcctcacctg gctcgacgag aaggtgcgct
     3001 tcggcttcac cgaatggcac tccgacgtct actaccaaaa ggacgtcacg ccgttgctct
     3061 cactggtcga attcgcgccc gacgaggatc ttgcgaatcg ggccgggatg atgctcgacg
     3121 tgttcctcct cgacatcgcc acgcacctgc aacgcgggac gttcggcgtc acgcacggcc
     3181 ggtcgtacat gaaggacaag agcatcgcga ccgacgagga caccttcgcc ctgtcgaagc
     3241 tgttgttcga cgacacgtcg ctgccgtaca acgagggtgc cgacgcgggc gcgaccctgt
     3301 tcgcccgggc ggagaacttc gcgctcgcgc ccgtcatcgg ggcgatcgcg acgagcgagg
     3361 agacgacggt cgaccaggag cacatgaacg tcccgctcga cccgtcggca ccggtcgacc
     3421 ccaacgtcgt cgcaccttac ggctacgact tcgacgatcc cgacaacgtc ccgttctggt
     3481 gggagcgggg cgcgcagacg gtgtggcagg aagtcccgat gacgatccag acgctcgacg
     3541 agtacaacct ctgggacagc gagttcttca gcccgttcaa gccgctgcgc gacgcggtcg
     3601 gcagcgacat ggccggcgcg cagaacctcg cgcagacgct cgcgccgcag ctcgcattcg
     3661 ggttgctcgc cgaggtgaac acgtacacgt accgcgcgcc gaacgtcatg ctgtcgaccg
     3721 cgcaggacta ccggcccggt gtgttcagcg agcagatcca gtcgtggcag gcgacgctcg
     3781 acgagagggc gctcgtgttc acgacgcacc cgaagaacga acccgaatcg ggcacggagt
     3841 ggcccgacag cgacggctac tggactggca ccggctcgat gccccgcagc gcgcagcacg
     3901 gcacggtctc ggtgaacatc tactcgccga cgtacacacc gaccggtccg ccgctcgacg
     3961 cgttcgggta cctcgattac acgcacgcgt acttcccgca ggagtacttc gacgaggtcg
     4021 tgcaggaggg caattggacg ttcggccgca agggcgacgg ctacgtcgcg ctgtggtcgc
     4081 agcggccgcc gacgtggcgc gagcacgatc cgaacgtcgt gttcacacac gggctgaccc
     4141 ggccgttcga cctcgttgca atgggcggcg cacagaacgt gtggatcgcg gaggtcggcg
     4201 acgccgcgaa gtgggagacg ttcgacgcgt tcaaggccgc gatcaccgcc gcgccggtga
     4261 cggtcgacgc cgacctgaac gtcgattaca cgtcaccaac cgaaggccac atgaggttcg
     4321 gctccaccgg cccgctcacg gtcaacgaca ccgaggtcga cctccacccg acggcgcgca
     4381 tcgacaaccc gttcgtcacc gtgcccttcg aaggcaagac ctacgagatc aaagccggcg
     4441 acgacaccct caccctcgac ttcgcaaact ggaaacggat gcagtgagcg cgtcgtagac
     4501 gcggtcgcgg atctcgttgg cttcgtcgcg ggtcatggtg cgggacggat ggcggaggac
     4561 gacgcggacg aggacgttct tctggccgtc gaccaggccg agccgctcac gtgcgatcgg
     4621 cggcagctcg gcgcccggcg tctcggacag caccgcgacc ttctcgacgg cgtccgcatc
     4681 cgcgccgagc gcgtggcgca cgcggtcgcc catgttctcg accgtgtcgt cgtcctccac
     4741 cgcgatcgag atgtcgcgcg tcacgggcgg gtggctcgac accggacggt agagcccgag
     4801 gtcgcacagc tggcctgcga cccgcgggtc gggatcgcgc agcagccgga tgtcggcgat
     4861 gcccttgcgc agcatgagga tgcgatcgag gcccaggccc atcgcgaggc ccgtgccctc
     4921 gacgtgcgcg ccccgcaaca cgtgctcgcc cgcgaggccg cactccccga tctcgaccca
     4981 ctcgtcgtcg ttgtcgacgt cgatctgcag gccgtgcgtc gtgtacgggt gctcgcgcgc
     5041 cgcgacgcgg tactcggcgc cgggcaggag cgtgcgcacg agcacgtcga tcatctcttt
     5101 gagatctccc gggccgagct cccgggttcg cgacacccgc cacaggtcga gttggtgcgg
     5161 cgtgcccgag tgcaagcggt cgatcgagtc ccgccggtac acgacaccag ggcaggcgag
     5221 cagcacgtcg tcgaccgggc ccagcgtccg cagcgcgccg gggatcagcg cggatgtgtg
     5281 gctgcgcagg acctggtcgg gtgcgacgta gcgcgtatag cgctcgtcac gtgtgacagc
     5341 gtcgggtgag tagccgaggc tgtcgtagtt gtcggcgagc gtgacgatgc gagtgccgcg
     5401 gtagacgcgg accgggcagt tccacgcgcg cgatagcgcg cgagtggcgt cgtcgacgag
     5461 gcgttggatc gcgtggcggc cttcggcggg atcggtcagg tctcgcagtg cgagcgcgcg
     5521 cgttgcttcg tcggcggtga gcagggtgat ggacatcgtg actccttggc gtacgtaccg
     5581 gcgaggggtg caatgccccc gggccggcga cgccggatgg agccgcgacc gttacgcgcg
     5641 agccccgcgg cgcccgtgaa gggcccgggg gcaacaaaat cgcggcgcac ggatcggtgt
     5701 cacggtggcc agggtagcgc accgccctga ccgccgtgcc cgacgacgag ctactgctgt
     5761 gcggacttga tcacgccgaa caccgcaccc tgcggatcgg ccagcaccgc gaagcggccg
     5821 gctgggatgt ccatcggcgg cgacaacacc gtggcacccg cggcttgcgc cttggcaacg
     5881 accgcgtcgg tgtcggcgac ttcgaagtac accagccagt gcggcggcat ccccggcatc
     5941 gcgggcttca tggcaccccc gatgtcgtgg ccgtcgagct tgaagagcgt gtagtccgtg
     6001 tcggggacgg gctcggtctc ccagccgagg agcttcc
//
LOCUS       DASUGA010000663         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1247308, whole genome shotgun sequence.
ACCESSION   DASUGA010000663 DASUGA010000000
VERSION     DASUGA010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247308"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /locus_tag="VFX21_13150"
     CDS             <1..1434
                     /locus_tag="VFX21_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX5096964.1"
                     /translation="SYGGTEFDTPNLDRFAAQATRFTQHYSGSLPCMPARHDILVGAL
                     DFLWRPWGSIELWEEPVTAGLRRSGVATKLVTDHPHLFEVGGENYHTDFTAWDYQRGH
                     ESDLWRTRPDASWIGAPLFGRRHMPYDNSRGWFRSEKDFPGPRVMSEAVRWVREESAA
                     HDSWLLFVDEFDPHEPFDTPEPYASMYDDSWDGPQHLVWPPYTVGAVEKGVLTERDAR
                     QVRAQYGSKLTMIDAWFGRLLDALSLDDTVVIVCTDHGHYLGEKDIFGKPGCPIYEPL
                     GHTPLLLSVPGVEAGTCDALTTNVDIHATLCDLFAVPPGYGAHGHSLLPLLRGDATAV
                     RDWALAGIWGREVHLIADHAKYVRAPDGENFPLSMWSNRWSTMPIHSAPDFKLPRPDD
                     RAYLDRMPGSTVPVIRQPFQPGDLLPFWAYSDFAAGTLAFDLGEDPSEERNLADDKLG
                     AQLSEMLHAALNEVDAPDDQFARLGFR"
     gene            1571..>2573
                     /locus_tag="VFX21_13155"
     CDS             1571..>2573
                     /locus_tag="VFX21_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096965.1"
                     /translation="MQVRHAAGRRRKFVLVALTLMVGAALWAWALGAGAAVSNPGPIT
                     VTIHDGTLSTALGSFSPLEGTATGTVDASGNITLPQANITFDEFDVNVTDPLPLTVHV
                     SPVAASAFTGNINPDTGLVTLSGSLTTNLDIATLGLTDCPLGPLSVTLSTATTGGVAY
                     DDATGEATLVDNTFVIPEIPAGQAGCGGLEGALNSTLGLPSTPGASTLTLPMTFSPTL
                     IGSTTTAAPATTTTTTTVASSTTTTTAAPTTTTTTTVPSSTTTTTAGPTTTTTAAPTT
                     TTSTTVAPTTTTTTAGPTTTTTTVPNNNCKPGWGYGDKNHCHTGPPGLAHSMRPFSRW
                     "
BASE COUNT          450 a          908 c          799 g          416 t
ORIGIN      
        1 agctacggcg gcaccgagtt cgacacgccg aacctggatc ggtttgctgc gcaggcgacg
       61 cgctttacgc agcattacag cggttcgctg ccgtgcatgc cggcgcgtca cgacatcctc
      121 gtcggtgcgc tcgacttcct ctggcgaccg tggggctcga tcgaactgtg ggaggagcct
      181 gttactgcgg ggttacgacg cagtggtgtc gccaccaagt tggtgaccga tcatccgcat
      241 ctgttcgagg tcggcggcga gaactaccac accgatttca cggcgtggga ctaccagcgc
      301 ggccacgaat ccgacctctg gcgcacgcgc cccgatgcgt cgtggatcgg cgcgccgctc
      361 ttcggccgcc ggcacatgcc ctacgacaac tcgcgcggct ggttccgttc cgagaaagac
      421 ttcccggggc cgcgcgtcat gagcgaggcc gtgcgctggg tgcgggaaga atcggccgca
      481 cacgactcgt ggttgttgtt cgtcgacgag ttcgacccgc acgagccgtt cgacaccccc
      541 gagccgtacg cgtcgatgta cgacgactca tgggacggcc cgcaacatct cgtgtggccg
      601 ccctacaccg ttggcgcagt cgaaaagggc gtgctgaccg agcgcgacgc acgccaagtg
      661 cgcgcgcaat acggcagcaa gctcacgatg atcgacgcgt ggttcggccg gctcctcgac
      721 gcgctctcac tcgacgacac cgtcgtgatc gtctgcaccg atcatggcca ctacttgggc
      781 gagaaagaca tcttcggcaa gcccggctgc ccgatctacg aaccgctcgg acacacgccg
      841 ctgttgcttt cggtgccggg cgtcgaggcc ggcacgtgcg acgcgctcac gacgaatgtc
      901 gacattcacg ccacgttgtg cgatctgttc gcagtaccgc ccgggtacgg cgcgcacggc
      961 cattcgttgc tcccgctcct gcgcggcgac gcaacggcgg tgcgggactg ggcgctagcc
     1021 ggcatctggg gccgcgaagt gcacctgatc gccgatcacg cgaagtatgt gcgtgcaccc
     1081 gacggcgaga acttcccgtt gtcgatgtgg tccaaccgct ggtcgacgat gccgattcac
     1141 agcgcgcccg acttcaagct gccgcgcccc gacgatcgcg cgtatctcga tcgcatgccg
     1201 ggctccacgg tgcctgtcat tcgccagccg ttccaacccg gcgacctgct gccgttctgg
     1261 gcttactcgg acttcgcggc cggcaccctc gcgttcgacc tcggcgagga tccgagcgag
     1321 gaacgcaacc tcgccgatga caagctcggc gcacagttgt ccgagatgct ccacgctgca
     1381 ctgaacgagg tcgatgcccc cgacgaccag ttcgcccgcc tcggctttcg ctgaccggcg
     1441 ctcgtacgcg agcggtcgcg cgcgttttgc ccgccttgat cggacccgtg gttccgacaa
     1501 gagcgccggt gctgcggcac atgggtagcg gggacccgga tatccgaacc acggacgagg
     1561 ggaactgcgt atgcaagtgc ggcacgcggc tggaagacgt cgcaaattcg ttctcgtcgc
     1621 gttgaccttg atggtcggcg cggcactctg ggcgtgggcg ctcggcgccg gcgcggcggt
     1681 gagcaatccc ggaccgatca ccgtcaccat tcacgacggc actctttcaa cggcgctcgg
     1741 gtcgttctct ccgctggagg gaaccgcaac gggaaccgtg gacgcctcgg gcaacatcac
     1801 gttgccgcag gccaacatca cgttcgatga gttcgacgtc aacgtcacgg atccgctgcc
     1861 gctgaccgtg cacgtctcgc ccgtcgccgc cagcgccttc accggcaaca tcaacccgga
     1921 caccgggctg gtcacgctgt cgggaagtct gacgaccaac ctggacatcg caacgctcgg
     1981 tttgaccgac tgcccgctcg ggccgctcag cgtcacattg agcaccgcga caaccggcgg
     2041 cgttgcctat gacgacgcaa ccggtgaggc gaccctcgtc gacaacacct ttgtgattcc
     2101 ggaaatcccg gccggccaag ccgggtgcgg cggtctcgaa ggtgcactca actcgacact
     2161 ggggttgccg tcgacgccag gtgcctcaac gttgacgctg ccgatgacgt tctcgccgac
     2221 cctcatcggt tcgaccacga ccgcagcgcc ggccacgacg accacgacca ccacggtcgc
     2281 gtcgagtacg acgacaacaa ctgcggcacc gaccacgacg acgacgacca ccgtcccgtc
     2341 gagcacgacc acgacgaccg ccggtccgac gacgacaaca actgccgcgc cgacgaccac
     2401 gacgtcgacc acggtggcgc cgacgacgac caccaccacg gctggtccga caaccacgac
     2461 cacgaccgtg ccgaacaaca actgcaagcc cggttggggc tacggcgaca agaaccactg
     2521 ccacaccggt ccgcctggct tggcgcactc gatgaggcca ttctcccgct ggt
//
LOCUS       DASUGA010000664         2458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_80862, whole genome shotgun sequence.
ACCESSION   DASUGA010000664 DASUGA010000000
VERSION     DASUGA010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80862"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="VFX21_13160"
     CDS             <1..914
                     /locus_tag="VFX21_13160"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX5096966.1"
                     /translation="ASHVWEERFGEHAYLPLVQAAFNDALKDAGIGPDALDHVIVTGV
                     HTRAARAASRALGVAPEAVVDDLASTIGFTGAAHVGLLLAGVLERAEPGQRVATVSIA
                     DGCEVVIGETTDRLGSVAPRRTLASQVAAGRPIASYQTFLTWRGFLDREPPRRPDPKS
                     PAAPPSFRAEEWKFGFTGSQCEVCGERHLPPARVCAQCRSIDQMVPVRMADMAGTVAT
                     FTVDRLTYSPSPPLVAVVIDFDGGGRYRCELTDVDAETVAIGDRVEMTFRRTYTSGGI
                     HNYFWKARPIRAGEGASVSERPMKTEV"
     gene            917..2116
                     /locus_tag="VFX21_13165"
     CDS             917..2116
                     /locus_tag="VFX21_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEX5096967.1"
                     /translation="MASHGIRDRVAIVGMGCTVFGEHWDKSADDLLVDAVTDALASAN
                     VDQAQIDAYWLGTMGSGISGLTLSRPLRVDYKPVTRVENMCATGSESFRNACYAVASG
                     AFDMVMATGVEKLKDGGFSGLTGASPPSDATTPELSAPALFSLLGPAYCKKYGVDADE
                     FKDVLTRIAWKNHRNGALNPRAQFRKEVSKETIACSPIMAGNLGIFDCSGVSDGAAAA
                     IIVRAEDAYEYTDQPIFVKGLSFVAGPAAGAVDRDYDYTTFQEVVAAAADAYQQAGVT
                     NPRAQLAMAEVHDCFTPTELVLMEDLGFAERGMAWKEVLAGTFDLDGELPVNPDGGLK
                     AFGHPIGASGLRMLFECWLQLRGEAGERQIKTVAEGKKLALTHNLGGQPGECVSYVGV
                     VGSEPAS"
     gene            complement(2130..>2458)
                     /gene="purE"
                     /locus_tag="VFX21_13170"
     CDS             complement(2130..>2458)
                     /gene="purE"
                     /locus_tag="VFX21_13170"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX5096968.1"
                     /translation="ERGLKVIIAGAGGAAHLPGMVASATPLPVIGVPVPLRHLDGMDS
                     LLSIVQMPAGIPVATVAIGNARNAGLLAVRILGITDGELRARMEAFQDDLADMVREKD
                     ERLQAD"
BASE COUNT          374 a          855 c          860 g          369 t
ORIGIN      
        1 cggcgtcgca cgtgtgggag gaacggttcg gcgagcacgc gtatctgccg ctcgtgcagg
       61 ccgcgttcaa cgacgccctg aaggacgccg gcatcgggcc cgacgcgctc gatcacgtga
      121 tcgtcaccgg tgtgcacacg cgcgccgcgc gcgccgcgtc acgcgcactc ggtgtcgcgc
      181 cagaagccgt cgtcgacgac cttgcgtcga cgatcggatt cacgggcgcc gcgcacgtcg
      241 gcctgttgtt ggcgggcgtg ctggaacgag ccgagcccgg gcagcgcgtc gcgacggtgt
      301 cgatcgccga cgggtgcgag gtcgtgatcg gcgagaccac cgatcggctc ggctccgttg
      361 cgccgcggcg cacgctcgcg tcgcaggttg cggcgggacg gcccatcgcg agctaccaga
      421 ccttcctcac ctggcgcggc ttcctcgacc gcgaaccgcc gcgtcgaccg gacccgaagt
      481 cgcctgcggc tccgccgtcg ttccgggccg aggagtggaa gttcggcttc accggttcgc
      541 agtgcgaggt gtgtggcgaa cggcacctgc cacccgcccg ggtgtgtgcg cagtgccgct
      601 cgatcgacca gatggtgccg gtgcgcatgg ccgacatggc gggcaccgtc gcaaccttca
      661 cggtcgaccg cctcacctac agcccgagcc cgcccctcgt ggcggtggtc atcgacttcg
      721 acggcggcgg ccgctatcgc tgcgagctca ccgacgtcga cgccgagacc gtcgccatcg
      781 gcgaccgggt cgagatgacg ttccgccgca cgtacacctc cggcgggatc cacaattact
      841 tctggaaggc gcgtccgatt cgcgcgggag agggagcgag cgtgagcgag cgacccatga
      901 agacggaggt ctgaacatgg ccagtcacgg gattcgcgat cgggtcgcca tcgtcgggat
      961 ggggtgcacc gtcttcggcg agcactggga caagagcgcc gacgacctgc tcgtcgacgc
     1021 cgtcaccgac gccctcgcgt cggccaacgt cgaccaagcg cagatcgacg cgtactggct
     1081 cggcaccatg ggctccggca tctcgggcct cacgctctcg cgtcctcttc gcgtcgacta
     1141 caagcccgtc acgcgcgtcg agaacatgtg cgccacggga tcggagtcgt tccgcaacgc
     1201 gtgttacgcg gtggcgtcgg gtgccttcga catggtgatg gcgaccggtg tcgagaagct
     1261 caaggatggc gggttctccg gcctgaccgg cgcgtcaccc ccgagcgacg ccaccacgcc
     1321 cgagttgagc gcgcccgcgc tgttcagcct gctcggtccc gcgtactgca agaagtacgg
     1381 cgtcgacgcc gacgagttca aggacgtgct cacgcgcatc gcatggaaga accatcgcaa
     1441 tggcgcgctg aacccgcgcg cgcagttccg caaggaggtg tcgaaggaga cgatcgcctg
     1501 ctcgccgatc atggccggca acctcgggat cttcgattgc tccggcgtgt ccgacggcgc
     1561 agccgccgcg atcatcgtgc gggccgagga cgcgtacgag tacaccgacc agccgatctt
     1621 cgtgaagggc ctgtcgttcg tggccgggcc ggccgccggc gcggtcgacc gcgactacga
     1681 ctacacgacg ttccaggaag tggttgccgc cgcggccgac gcgtaccagc aagcgggtgt
     1741 cacgaacccg cgtgcgcagc tcgccatggc agaagtgcac gactgcttca cgccgaccga
     1801 gctcgtgctc atggaggacc tcgggttcgc cgagcgcggc atggcgtgga aggaggtgct
     1861 cgcgggcacg ttcgacctcg acggcgagct cccggtgaac cccgacggcg ggctcaaggc
     1921 gttcgggcac ccgatcggcg cgtcgggtct gcgcatgctg ttcgagtgct ggctgcaact
     1981 gcgcggtgag gccggtgaac ggcagatcaa gacggtggcc gagggcaaga agctcgcgct
     2041 cacccacaac ctgggcggcc agcccggcga gtgcgtcagc tacgtcggcg tcgtggggag
     2101 cgaacccgcc tcctgacccc cgttcgcggt cagtcggcct ggaggcgctc gtccttctcg
     2161 cggaccatgt cggcgagatc gtcctggaac gcttccatgc gcgcgcgcag ctcgccgtcg
     2221 gtgatgccga ggatgcgcac cgcgagcagg cccgcattgc gcgcgttgcc gatggccacg
     2281 gtggcaaccg ggatcccggc cggcatctgc acgatcgaca gcaacgagtc catcccgtcg
     2341 aggtgccgca gcggcaccgg gaccccgatc accggcagcg gcgtcgccga ggcgaccatg
     2401 ccgggcaggt gggcggcgcc tcccgccccg gcgatgatca ccttcaggcc tcgttcct
//
LOCUS       DASUGA010000665         2253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1974147, whole genome shotgun sequence.
ACCESSION   DASUGA010000665 DASUGA010000000
VERSION     DASUGA010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1974147"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VFX21_13175"
     CDS             <1..208
                     /locus_tag="VFX21_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008386320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5096969.1"
                     /translation="VAALGAIGDPAGLPAVLAACDDKPAVRRRAVLALAPFTGPEVDA
                     ALARAHDDRDWQVRQAAEDLQERD"
     gene            complement(242..1249)
                     /locus_tag="VFX21_13180"
     CDS             complement(242..1249)
                     /locus_tag="VFX21_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX5096970.1"
                     /translation="MPLDLAIDLGTANTLVYARGRGIILFEPTVIALNSRTQDVLAMG
                     NQAWHMIGRTPGHIVAVRPLRGGAITDFEITQRLIRLIFERSGVNRLQRARVLVCVPS
                     GITHVEQRAVLEATRRAGASQTYLIEQPMAAAIGAGLPIHEPLGNMVVDIGGGTSEVA
                     VISLGGIVALRAVRVGGFDLDHAIQAHLRRVHNVAVGERTAEEVKLAIGSAYPTPEEY
                     KAEVRGRDLMSGMPRTVVLEPEEIREAIAEPVQAVQHAVIQALAETPPELAQDLLEVG
                     IHLVGGGAMLRGLDRLIARETGVPVRLVDMPLETVVLGAGRCLESFDRLRDMFMDRTS
                     R"
     gene            complement(1268..1765)
                     /locus_tag="VFX21_13185"
     CDS             complement(1268..1765)
                     /locus_tag="VFX21_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX5096971.1"
                     /translation="MPVVPPESNDPSAPADWIALTNEVLPSEAATAWATTSRAGAVVC
                     FLGVVRDHAEGRDGVTALTYEAYEDAALARLGEIADETRRRWPEVERLALLHRLGHLE
                     LSEASVLVVASSPHRAEAFEAARFAIDTLKETVPIWKQEHWDGGTAWSEAAHDVRPVR
                     ELRPA"
     gene            1859..>2253
                     /locus_tag="VFX21_13190"
     CDS             1859..>2253
                     /locus_tag="VFX21_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096972.1"
                     /translation="MPADDDEASGPPPHPLDRVWFHPSELGAPVTALRPQPAAPRVWV
                     VAALALVIGILGTLGVVSVVGTFDNGTPTNRLQRTVATPVDTERIETLVQSTFRSITR
                     VSVVPATGGDAAVVGSGVAIGDGRILTAA"
BASE COUNT          313 a          816 c          792 g          332 t
ORIGIN      
        1 cgttgccgcc ctcggcgcca tcggcgaccc cgccggcctt cccgcggtgc tcgccgcgtg
       61 tgacgacaag cccgcggtcc gtcgtcgcgc ggtgctcgcg ctcgcgccgt tcacggggcc
      121 cgaggtcgac gccgcgctcg cacgcgcgca cgacgaccgc gactggcagg tgcgtcaggc
      181 cgcggaggac ttgcaggaac gcgactgacg tcgcgcgcgg ttcagactgt gcagcgcggg
      241 atcagcgcga cgttcggtcc atgaacatgt cgcgcagccg gtcgaagctc tcgaggcacc
      301 gccccgcgcc gagcaccacg gtctcgagcg gcatgtcgac caaccggacg ggcaccccgg
      361 tctcgcgcgc gatcaagcgg tcgaggccgc gcagcatcgc gccaccaccc acgaggtgga
      421 tgccaacttc gagcaggtcc tgcgcgagct cgggcggcgt ctctgcgagc gcctggatca
      481 cggcgtgctg cactgcctgc accggttccg cgatcgcctc gcggatctct tccggctcga
      541 gcacgacggt gcgcggcatg ccgctcatca agtcgcgacc gcgcacctcc gccttgtact
      601 cctcgggcgt ggggtacgcg gagccgatcg cgagcttcac ttcttccgcg gtgcgttcac
      661 cgacggcgac gttgtgcacg cgtcgcaagt gcgcctggat cgcgtggtcg agatcgaacc
      721 cgccgacgcg cacggcgcgc aacgcgacga tgccgccgag tgagatcacc gcgacctcgc
      781 tcgtgccacc gccgatgtcc acgaccatgt tgccgagcgg ttcgtggatc ggcagacccg
      841 cgccgatcgc ggcggccatc ggttgctcga tgagataggt ctgcgacgcg cccgcgcgtc
      901 gcgtggcctc gaggaccgcg cgttgctcga cgtgcgtgat gcccgacgga acgcacacga
      961 ggacgcgcgc gcgttggagg cggttcacgc cggagcgctc gaagatcagc cggatcaggc
     1021 gctgggtgat ctcgaagtcg gtgatggcgc cgccccggag gggacgcacg gccacgatgt
     1081 gccccggggt gcggccgatc atgtgccagg cctggttgcc catggccagc acgtcctgcg
     1141 tgcggctgtt cagggcgatg acggtgggct cgaagaggat gatcccccgg ccccgtgcgt
     1201 acacgagggt gttcgcggtc ccgaggtcga tggcgaggtc gagcggcacg gcgggctctc
     1261 ggtccggtca ggcgggccgg agctcgcgga cggggcgcac gtcgtgcgcg gcctcggacc
     1321 atgcggtccc gccgtcccag tgctcctgct tccagatcgg cacggtttcc ttcagggtgt
     1381 cgatggcgaa ccgtgcagct tcgaacgcct cggctcggtg gggcgacgat gcaaccacga
     1441 gcaccgacgc ttccgacaac tcgagatgac cgagacggtg caacaacgcg agccgctcga
     1501 cctcgggcca gcggcgacgc gtctcgtcgg cgatctcacc gagccgcgcg agcgcggcgt
     1561 cttcgtacgc ctcgtacgtg agcgcggtga caccgtcgcg accttcggcg tgatcgcgca
     1621 cgacgccgag gaaacagacg accgcacccg cgcgcgacgt ggtggcccac gcggtggctg
     1681 cctcggaagg cagcacctcg ttcgtgagtg cgatccagtc ggccggggcc gacgggtcgt
     1741 tcgactcggg tgggacgacc ggcacggctg ctcactctat cggcgcattg cgggccctat
     1801 accagccgat cccgagggcg cggcggatgg gccgccgggc gtcagtacac tccgcgtcat
     1861 gcccgccgac gacgacgagg cgagcgggcc gccgccgcac ccgctcgacc gggtctggtt
     1921 ccacccttcc gagctcggcg ctccggtcac ggccctccgg ccgcaacccg ccgcgccgcg
     1981 cgtgtgggtc gtcgccgcgt tggcgctggt catcggcatt ctcggcaccc tcggcgtcgt
     2041 cagcgtcgtc ggcacgttcg acaacggcac ccccacgaat agactccagc gcactgtcgc
     2101 cacaccggtc gacaccgagc gcatcgagac gctcgtgcaa tcgacgttcc gcagcatcac
     2161 gcgcgtctcg gtggttcccg cgaccggtgg cgacgccgcc gtcgtcggct cgggcgtcgc
     2221 gatcggcgat ggtcggatcc tcaccgccgc cca
//
LOCUS       DASUGA010000666         2221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_203917, whole genome shotgun sequence.
ACCESSION   DASUGA010000666 DASUGA010000000
VERSION     DASUGA010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203917"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VFX21_13195"
     CDS             complement(<1..360)
                     /locus_tag="VFX21_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HEX5096973.1"
                     /translation="MRVSPLDARHRALDARLLPFGGWDMPIQYRSVLDEHRACREDAA
                     VFDVSHLGTIECADPDTFELLQWLLTNDLHRIKPGRAQYTHLLDPDDAHVVDDIIVWW
                     AAPGEFLVMPNASNTAPL"
     gene            complement(377..829)
                     /locus_tag="VFX21_13200"
     CDS             complement(377..829)
                     /locus_tag="VFX21_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096974.1"
                     /translation="MNSEQLPHETVRFAHNSERQFAKLLDFYNVAWEYEPRTFTLEWD
                     NDGQPAQAFSPDFYLPAYDLYIEITTLNQKLVTKKNRKARRLRELHPDISIRVLYQRD
                     YLHLLVKYGLEPPSQLVDAGLPADGPPLRLDLDRRPRTAHPDTTAESA"
     gene            complement(837..1310)
                     /locus_tag="VFX21_13205"
     CDS             complement(837..1310)
                     /locus_tag="VFX21_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013018968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5096975.1"
                     /translation="MSELVGYRGPQVCKIVGITYRQLDYWARTELVRPSVCDAQGSGT
                     QRLYSYRDLVELKVVKRLLDAGISLQSARKAIEYLRTQGEDLASAHLVIDGNTPMLAL
                     DENQLIDLMRAGQGVLNVVKLGPVMSEVDTQLDLFAPAANGDVMRDHPSNRKAAK"
     gene            complement(1445..1927)
                     /locus_tag="VFX21_13210"
     CDS             complement(1445..1927)
                     /locus_tag="VFX21_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017604907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX5096976.1"
                     /translation="MIRLSLIGVRVEVPTNQPIVLLREDGGARFLPIFIGSPEATAIV
                     YAMQGLETPRPMTHDLFKTVLDGLEVSLERVEITELHDGTFYAEIELVRNGTKSRISS
                     RPSDAIALAVRYGGTIPIFAHEDVLEEAGVLFEEDQEEQEVEQFREFLDQVTPEDFAQ
                     "
     gene            complement(1924..>2221)
                     /locus_tag="VFX21_13215"
     CDS             complement(1924..>2221)
                     /locus_tag="VFX21_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096977.1"
                     /translation="LAYLMSQLADRHPRRLSACALLDRRARRIVPQDVAYCGFELADE
                     FVLGYGLHVRDLYRNLPFVVTGARDDVVAAPDCYVPKLYKGGGDGAGAGGSVTS"
BASE COUNT          381 a          704 c          748 g          388 t
ORIGIN      
        1 gagcggtgcg gtgttggagg cgttgggcat cacgaggaat tcgcccggcg cggcccacca
       61 cacgatgatg tcgtcgacga cgtgcgcgtc gtctgggtcg agcaagtgcg tgtactgggc
      121 ccgcccgggc ttgatgcgat gcaggtcgtt cgtgagcagc cactgcaaca gctcgaacgt
      181 atcgggatcc gcgcactcga tcgtccccag gtgcgagacg tcgaagacag ccgcgtcctc
      241 gcggcaggcc cggtgctcgt cgagcacgct gcggtactgg atcggcatgt cccagccacc
      301 gaagggcagc aagcgggcat cgagcgcccg gtggcgcgca tccagcggag agactcgcat
      361 tagtgcacca ctgaagttac gccgattcgg cggtcgtatc gggatgcgcc gtacgcggcc
      421 ggcggtcgag gtcgagccgc aacggcggac cgtccgcggg cagtcccgca tcgacgagtt
      481 gcgacggcgg ttctaacccg tacttcacga gcagatgcaa gtagtcgcgc tggtacagca
      541 cccggatcga gatgtcgggg tgcagctcgc gcagccggcg cgctttgcgg ttcttcttcg
      601 tcacgagctt ctggttcagc gtcgtgatct cgatgtagag gtcgtacgcg ggcaggtaga
      661 agtccgggga gaacgcctgc gcgggttgtc cgtcgttgtc ccactcgagc gtgaacgtgc
      721 gcggctcgta ctcccacgcg acgttgtaga agtcgagcag cttcgcgaat tgccgttcgg
      781 agttgtgggc gaagcgaacg gtctcgtgcg gcagctgctc ggaattcaca cagcctctac
      841 ttggctgcct tgcggttcga cggatggtca cgcatgacgt cgccgttcgc ggccggggcg
      901 aacaggtcga gctgcgtgtc gacttcgctc atgaccggac ccagcttcac gacgttcagc
      961 acaccctgac ccgcgcgcat caggtcgatg agctggttct cgtcgagggc gagcatcggc
     1021 gtgttgccgt cgatgacgag gtgcgcggac gccaggtctt caccctgggt gcgcaggtac
     1081 tcgatcgctt tgcgggccga ctgcaacgag atgcccgcgt cgagcagccg cttgacgacc
     1141 ttgagctcga cgaggtcgcg gtaggagtac agccgctgcg tgccactccc ctgcgcgtca
     1201 cacaccgacg ggcggacgag ctcggtgcgc gcccagtagt cgagctggcg gtacgtgatg
     1261 ccgacgatct tgcagacctg cggtccgcga tagccaacga gctcgctcat ctggacatga
     1321 ccccccgggg gaagttgtgg tcacacgaga aacaacagtg taactacaac ggtgagggta
     1381 agcgtcaggt cgaggcccgg tcaatgtcgg gctgaggtag cagcacgccg acccgggccc
     1441 aggcctactg cgcgaagtcc tcgggggtga cctggtcgag gaactcccgg aactgctcga
     1501 cctcttgttc ctcttggtct tcttcgaaga gcactcccgc ctcctcgagc acgtcttcgt
     1561 gggcgaagat cgggatcgtg ccgccgtaac gcaccgcgag cgcgatcgca tcggacggtc
     1621 gggacgagat ccgcgacttg gtgccgttac gcaccaattc gatctccgcg tagaacgtgc
     1681 cgtcgtgcag ctcggtgatc tccacccgtt cgagcgacac ttcgagcccg tcgagcaccg
     1741 tcttgaacag gtcgtgggtc atcggccgtg gcgtctcgag cccctgcatg gcgtagacga
     1801 tcgcagtcgc ctcgggcgac ccgatgaaga tcggcaggaa ccgcgcgccg ccgtcttcgc
     1861 gcaacagcac gatcggctgg ttcgtcggga cctcgacgcg cacgccgatg agcgacaggc
     1921 ggatcacgag gtcacgctac caccggcccc ggcgccgtct cccccgcctt tgtagagctt
     1981 gggcacgtaa caatcaggcg cggcaacgac gtcgtctcgc gcgcccgtga ccacgaatgg
     2041 aaggttgcgg tacaagtcgc gcacgtgcaa tccgtacccc agaacgaact cgtcggcgag
     2101 ctcgaatccg caatacgcga cgtcttgcgg gacgattcgc cgcgctctgc ggtcgagcag
     2161 cgcgcacgcc gacagccgcc ggggatgacg gtcggcgagc tggctcatga gatatgcgag
     2221 c
//
LOCUS       DASUGA010000667         5769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1157568, whole genome shotgun sequence.
ACCESSION   DASUGA010000667 DASUGA010000000
VERSION     DASUGA010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157568"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VFX21_13220"
     CDS             <1..375
                     /locus_tag="VFX21_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096978.1"
                     /translation="GQARARGRYFRIRKRTSHITLVVARYDEDRLEEIRRVEETSGRG
                     AAVQQRRRAERVRRSRAAAAPEAHDHDHDHEHEEDVPVASEEVVEEIEELEEEIEAGE
                     AEIEAVDEVETETEDDGEEETK"
     gene            375..1298
                     /gene="rpsC"
                     /locus_tag="VFX21_13225"
     CDS             375..1298
                     /gene="rpsC"
                     /locus_tag="VFX21_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX5096979.1"
                     /translation="MGQKVNPYGFRLGVTTDWKSRWIADKKEYRDFLIEDWRVRDYLR
                     KQLERAAVSRIEIERTRDRLRIDVYTARPGIVIGRRGAEAERLREGLRKITGNPKIQF
                     NIQEIKQPEIDATLLAQGVADQLAGRVSFRRAMKRAVQTAMKAGALGVRVQCAGRLGG
                     AEMSRKEWYREGRVPLHTLRADVDYGLAEARTTFGRIGVKVWIYKGEILPYKSAAEDK
                     IAKEAAMAVGETAGQAPGRRVVSAGGGRRKAPAPESAPVEASDSDSDKPTRELVHEAD
                     PELERLLAEEEEIERRTRDHHETPHFRGGED"
     gene            1300..1716
                     /gene="rplP"
                     /locus_tag="VFX21_13230"
     CDS             1300..1716
                     /gene="rplP"
                     /locus_tag="VFX21_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX5096980.1"
                     /translation="MLMPRKVRHRKQQRGRLTGLAKGGTKLAFGDYGIQALEPGWITN
                     RQIEAARIAMTRHIKRGGKVWINVFPDKPITQKPAETRMGSGKGNPEKWVAVVKPGRV
                     LFELSGVAEPVAREAMRLAIHKLPIKARFIVRTEEA"
     gene            1799..2011
                     /gene="rpmC"
                     /locus_tag="VFX21_13235"
     CDS             1799..2011
                     /gene="rpmC"
                     /locus_tag="VFX21_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX5096981.1"
                     /translation="MTKASEFREMTDEVLTDHLAQSKDELFKIRFQHATGQLDNYKRI
                     GELRRDVAKIETLLREREIAAHEAQE"
     gene            2014..2292
                     /gene="rpsQ"
                     /locus_tag="VFX21_13240"
     CDS             2014..2292
                     /gene="rpsQ"
                     /locus_tag="VFX21_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX5096982.1"
                     /translation="MADETTTEARGNTRKVREGIAVGTKMDKTVVVAVTSRVRHPRYR
                     KTVQRTKKLYAHDETNDVREGDRVRVAETRPMSKQKRWRIVEVLERAR"
     gene            2289..2657
                     /gene="rplN"
                     /locus_tag="VFX21_13245"
     CDS             2289..2657
                     /gene="rplN"
                     /locus_tag="VFX21_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX5096983.1"
                     /translation="MIQQESRLRVADNSGAREVLCIKVLGGTRRRYASIGDIFVATVK
                     DAVPGASVKKGDVVKCVVVRTKKEKRRPDGSYIRFDENAAVLINDQMQPRGTRIFGPV
                     GRELRDRKFMRIVSLAPEVL"
     gene            2657..2971
                     /gene="rplX"
                     /locus_tag="VFX21_13250"
     CDS             2657..2971
                     /gene="rplX"
                     /locus_tag="VFX21_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX5096984.1"
                     /translation="MKLKKGDEVVVIAGKDVGKRGEITAVFPDRDKVIVTSSQLINVA
                     KKHQKQTRATMQGGIIDKEMPIHVSNVALVHSSCGATRIGYRFTPDGKKIRVCKKCGS
                     DV"
     gene            2971..3540
                     /gene="rplE"
                     /locus_tag="VFX21_13255"
     CDS             2971..3540
                     /gene="rplE"
                     /locus_tag="VFX21_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX5096985.1"
                     /translation="MADTKERPRLRQRYDSELRPALKDELGLGNVMQVPRLTKIVVNM
                     GVGRATQQRSLLEGAVTDMTIITGQKPLVVKAKKSIASFKLREGNEIGVKVTLRGDRM
                     WEFYDRLVSLAIPRIRDFRGMSPKQFDGRGNYTFGLNEQLVFPEINYDKIDTVRGMDI
                     TLVTTAETDDHGRALLRALGFPFRRDGQG"
     gene            3546..3731
                     /locus_tag="VFX21_13260"
     CDS             3546..3731
                     /locus_tag="VFX21_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX5096986.1"
                     /translation="MAKKALINKSQRTPKFKVRAYTRCRRCGRARSVYRHFGLCRVCL
                     RQMGHAGEIPGLTKSSW"
     gene            3748..4152
                     /gene="rpsH"
                     /locus_tag="VFX21_13265"
     CDS             3748..4152
                     /gene="rpsH"
                     /locus_tag="VFX21_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018505119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX5096987.1"
                     /translation="MTMTDPIADMLTRIRNANIAFHDDVIMPSSKLKEAVAKILEQEG
                     YIQGFSVDEQPGRPGRRLSIKMKYTNDRRRTISGLKRVSRPGLRVYSKADRVPRVLGG
                     MGIAILSTNIGILTDREARQRKVGGEILCQVW"
     gene            4164..4703
                     /gene="rplF"
                     /locus_tag="VFX21_13270"
     CDS             4164..4703
                     /gene="rplF"
                     /locus_tag="VFX21_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019818480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX5096988.1"
                     /translation="MSRIGKQPITVPSGVDVNVSGTKVTVKGPKGQLEHDLIGGVAVE
                     RDGDTLTVTRVDDSRSNRSLHGLQRTLVNNMVVGVSEGFSKELEIVGVGYRATAQGQD
                     AMELALGFSHPVKVKAPAGITFEVPAPTRIVVRGFDKQLVGQVAADIRKIRKPEPYKG
                     KGVRYVDERILRKAGKSAK"
     gene            4718..5074
                     /gene="rplR"
                     /locus_tag="VFX21_13275"
     CDS             4718..5074
                     /gene="rplR"
                     /locus_tag="VFX21_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005505006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX5096989.1"
                     /translation="MALTKQEQRRRRHRRVRKKVLGTAERPRIAVFRSSKHIYVQAID
                     DIAGRTVASASTMEASVRGGSTATVAAAKNVGKQLGERVKAAGASAAVFDRGGFKYHG
                     RVAALADGAREAGLEL"
     gene            5075..5626
                     /gene="rpsE"
                     /locus_tag="VFX21_13280"
     CDS             5075..5626
                     /gene="rpsE"
                     /locus_tag="VFX21_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX5096990.1"
                     /translation="MPEGQYDERVIAINRVAKVVKGGRRFSFTALVVVGDGAGNVGLG
                     YGKAKEVPAAIQKGMEEAKKDLFAVPLAGETITHVVLGEHDAARVLLKPASPGTGVIA
                     GGAARAILEAAGIHDVLAKSLGSSNAINVARATMQGLRELRRPEEVAKLRGKSAEEVS
                     TKGMLEAYRERERAGKHTVTEVA"
     gene            5623..>5769
                     /gene="rpmD"
                     /locus_tag="VFX21_13285"
     CDS             5623..>5769
                     /gene="rpmD"
                     /locus_tag="VFX21_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX5096991.1"
                     /translation="MIMGQLKVTLVKSKIGTKPKHRGTVRALGLGRIGSSNVLPDRPE
                     IRGMV"
BASE COUNT         1115 a         1851 c         2003 g          800 t
ORIGIN      
        1 ggtcaggcgc gcgcccgcgg ccggtacttc cggatccgca agcgtacgtc gcacatcacg
       61 ctcgtcgtgg ctcgctacga cgaggaccgc ctcgaggaga tccggcgggt cgaggagacg
      121 agcgggcggg gtgccgcggt gcaacagcgc cgccgggccg agcgcgtccg tcgctcccgc
      181 gccgcggccg cacccgaggc gcacgatcac gatcacgacc acgagcacga agaagacgtg
      241 cccgtcgcga gtgaagaggt cgtcgaggag atcgaagagc tcgaagaaga gatcgaggcc
      301 ggcgaggccg agatcgaagc cgtcgatgag gtcgaaaccg agaccgagga cgacggcgaa
      361 gaggagacca agtaatgggt cagaaggtca acccctacgg cttccgcctc ggtgtcacca
      421 ccgactggaa gtcacgctgg atcgccgaca agaaggaata ccgcgacttc ctcatcgagg
      481 actggcgcgt tcgcgactac ctgcgcaagc agctcgaacg cgccgcggtg agccgcatcg
      541 agatcgagcg cacgcgtgac cgcttgcgca tcgacgtcta caccgcgcgt cccggcatcg
      601 tcatcggtcg ccggggtgcg gaggccgagc gcctgcgtga aggtctgcgc aagatcaccg
      661 gcaacccgaa gatccagttc aacatccaag agatcaagca gcccgagatc gacgcgacac
      721 tgctcgcgca gggtgtcgcc gaccagctcg ccggccgtgt gtcgttccgc cgggcgatga
      781 agcgtgcggt gcagaccgcg atgaaggccg gggcgctcgg tgtccgcgtg cagtgcgccg
      841 gtcgtctcgg cggcgccgag atgagccgca aggagtggta ccgcgaaggt cgggtgccgc
      901 tgcacacgct gcgcgccgac gtcgactacg gcctcgccga agcgcgcacc acgttcggtc
      961 gcatcggcgt gaaggtgtgg atctacaagg gcgagatcct cccctacaag agcgccgctg
     1021 aagacaagat cgcgaaggaa gccgcgatgg ccgtcggcga gaccgcgggc caggcgcccg
     1081 ggcgtcgcgt cgtcagcgcc ggtggtggtc gtcgcaaggc gcccgctccc gagtcggcac
     1141 cggtcgaagc gagcgacagc gacagcgaca agcccacgcg agagctcgtg cacgaggccg
     1201 atcccgagct cgagcgcttg ttggccgagg aagaggagat cgagcggcgc acgcgcgatc
     1261 accacgagac gccgcacttc cggggcggtg aggactgaca tgttgatgcc ccgcaaggtc
     1321 cgtcaccgca agcagcagcg cggtcgcctg accgggctcg cgaagggcgg cacgaagctc
     1381 gcgttcggcg actacggcat tcaggcgttg gagccgggct ggatcacgaa ccgccagatc
     1441 gaggccgctc gtatcgcgat gacccgtcac atcaagcgtg gcgggaaggt gtggatcaac
     1501 gtcttccccg acaagccgat cacgcagaag ccggcggaga ctcgcatggg ttccggcaag
     1561 ggcaaccccg agaagtgggt tgcggtcgtg aagccgggcc gcgtgctgtt cgagctctcg
     1621 ggcgtcgcgg aaccggtcgc gcgcgaggcg atgcggctcg cgattcacaa gctgccgatc
     1681 aaggcgcgct tcatcgtgcg caccgaggag gcgtgacgat gaccggccag gcgcagatcc
     1741 ggaggcccgg tggccgcacc ggctccgtcg atgttcgagg gccggaggtc gcagcccaat
     1801 gaccaaggcc tccgaattcc gcgagatgac cgacgaagtg ctcaccgacc acttggccca
     1861 atcgaaggac gagctgttca agatccggtt ccagcacgcg accggtcagc tcgacaacta
     1921 caagcgcatc ggggagctgc ggcgcgacgt cgcgaagatc gagacgctcc tgcgtgaacg
     1981 cgagattgcc gcccacgagg cacaggagta gcgatggcgg acgagaccac caccgaagcg
     2041 cggggcaaca cccgcaaggt gcgcgaaggc atcgccgtcg gcacgaagat ggacaagacc
     2101 gtcgtcgtgg ccgtgacgag ccgggtgcgt cacccccgct accgcaagac ggtgcaacgc
     2161 acgaagaagc tctacgcgca cgacgagacc aacgacgtgc gcgagggcga ccgcgtgcgc
     2221 gtcgccgaga cgcggccgat gtcgaagcag aagcgctggc gcatcgtcga agtactcgag
     2281 cgtgcccgat gatccagcaa gagtcgaggc ttcgtgtcgc cgacaactcc ggcgcacgcg
     2341 aggtcctgtg catcaaggtg ctcggcggca cccgccggcg ttacgcgagc atcggcgaca
     2401 tcttcgtggc gaccgtgaaa gacgcggtgc cgggtgcgtc ggtgaagaag ggcgacgtcg
     2461 tgaagtgcgt cgtcgtccgc acgaagaagg aaaagcgccg tcccgacggc agctacatcc
     2521 gcttcgacga gaacgccgca gtgttgatca acgaccagat gcagccgcgg ggcacccgca
     2581 tcttcggtcc ggtcggacgg gagctgcgcg accgcaagtt catgcggatc gtgtcgctcg
     2641 cgccggaggt cttgtaatga agttgaagaa gggcgacgag gtcgtcgtga tcgcgggcaa
     2701 agacgtcggc aagcgcggcg agatcactgc cgtgttcccc gaccgcgaca aggtgatcgt
     2761 caccagctcg cagctgatca acgtcgcgaa gaagcaccag aagcagacgc gcgccacgat
     2821 gcagggcggc atcatcgaca aggagatgcc gatccacgtc tcgaacgtcg cgctggtgca
     2881 cagcagctgc ggcgcgacgc gcatcggcta ccgcttcacg cccgacggca agaagatccg
     2941 cgtgtgcaag aagtgcggga gcgacgtgtg atggccgaca ccaaagaacg tccgcgcctg
     3001 cggcagcgtt acgacagcga gctccgtccc gcgttgaagg acgagctcgg cctcggaaac
     3061 gtcatgcagg tcccgcggct cacgaagatc gtcgtcaaca tgggcgtcgg ccgcgcgacg
     3121 cagcagcgtt cgctgctcga gggcgcggtc accgacatga cgatcatcac cggccagaag
     3181 ccgttggtgg tcaaggcgaa gaagtcgatc gcgagcttca agctccgcga gggcaacgag
     3241 atcggcgtca aggtgacgct gcgcggcgac cggatgtggg agttctacga ccggctcgtc
     3301 agcctggcga tcccccgtat ccgtgacttc cgcgggatgt cgccgaagca gttcgacggg
     3361 cgcggcaact acacgttcgg tttgaacgag cagctcgtgt tccccgagat caactacgac
     3421 aagatcgaca ccgttcgagg catggacatc acgctcgtga cgactgccga gaccgacgac
     3481 cacggccgcg cgcttctgcg ggcgctgggt tttccgttcc gccgcgatgg ccaggggtag
     3541 gaccgatggc gaagaaagca ctcatcaaca agtcccagcg cacaccgaag ttcaaggtgc
     3601 gcgcgtacac gcgctgccgt cgttgcgggc gcgcgcgttc ggtctaccgg cacttcggcc
     3661 tctgccgcgt gtgcctgcgc cagatggggc acgccggcga gattcccggc ctgacgaaga
     3721 gctcttggtg aggggggtgg cagcgaagtg acgatgaccg acccgatcgc cgacatgctc
     3781 actcgtattc ggaacgcgaa catcgcgttc cacgacgacg tcatcatgcc gtcctcgaag
     3841 ctcaaagagg ccgttgcgaa gatcctggag caagagggat acatccaggg tttctcggtc
     3901 gacgagcagc cgggccgtcc cggtcggcgc ctcagcatca agatgaagta cacgaacgac
     3961 cgccggcgca cgatctcggg cttgaagcgc gtgtcgcgtc ccggcctgcg cgtgtactcg
     4021 aaggccgatc gcgtgccgcg cgtcctgggt ggcatgggca tcgcgatctt gtccaccaac
     4081 atcgggatcc tcaccgaccg cgaagcgcgc cagcgcaagg tcggcggcga gatcctctgt
     4141 caggtctggt agcggagcac gtcatgtctc gtatcgggaa gcaacccatc acggtgccga
     4201 gcggcgtcga cgtcaacgtc tcgggcacga aggtcaccgt gaagggtccg aagggccagc
     4261 tcgagcacga cctcatcggt ggtgtggccg tcgagcgcga cggtgacacg ctcaccgtga
     4321 cgcgcgtcga cgactcgcgc tcgaaccggt cgttgcacgg cctccagcgc acgctggtca
     4381 acaacatggt cgtcggcgtc tcggagggct tctccaagga gctcgagatc gtcggcgtcg
     4441 gttaccgcgc cacggcgcag ggccaggacg cgatggagct cgcactcggg ttctcgcacc
     4501 cggtcaaggt gaaagcgccg gccggcatca cgttcgaagt gccggcgccg acgcgcatcg
     4561 tcgtgcgcgg cttcgacaag cagctcgtcg gtcaagttgc cgccgacatc cgcaagatcc
     4621 gcaagcccga gccgtacaag ggcaagggcg tccgctacgt cgacgagcgc atcctgcgca
     4681 aggccggcaa gagcgcgaag tagcaggtca acgaaccatg gcactcacca agcaagaaca
     4741 gcgtcgacgc cgtcaccggc gggtgcgcaa gaaggtcctc ggcaccgccg agcggccgcg
     4801 catcgcggtg ttccgttcca gcaagcacat ctacgtgcag gcgatcgacg acatcgcggg
     4861 ccgcaccgtc gcgagcgcgt cgacgatgga agcgtcggtg cgcggcggtt cgaccgcgac
     4921 cgtcgcggcc gcgaagaacg tcggcaagca gctcggcgaa cgcgtgaagg cggcgggcgc
     4981 gtccgccgcg gtgttcgacc gcggcggctt caagtaccac ggccgcgtcg cggcactcgc
     5041 agacggtgcc cgcgaagcgg gattggagct ctaaatgcct gagggtcagt acgacgagcg
     5101 cgtcatcgcc atcaaccgcg tcgccaaggt cgtcaagggt ggccggcggt tctcgttcac
     5161 cgcgctggtg gtcgtcggcg acggcgccgg caacgtcggg ctcggttacg gcaaagccaa
     5221 ggaagtccct gccgccatcc aaaagggcat ggaagaagcg aagaaagact tgttcgcggt
     5281 gccgctcgcg ggcgagacga tcacccacgt cgtgctcggc gagcacgacg cggcccgcgt
     5341 gctgctgaag cccgcgtcgc ccggtaccgg tgtcatcgcc ggtggcgcgg cgcgcgccat
     5401 tctcgaagcg gcgggcatcc acgacgtgct ggcgaagtcg ctcggttcgt cgaacgcgat
     5461 caacgtcgcg cgcgccacga tgcaaggtct gcgcgagctg cgccgccccg aagaggtcgc
     5521 gaagttgcgc ggcaagtcgg ccgaagaagt gtccacgaag ggcatgctcg aggcgtaccg
     5581 cgaacgggaa cgggctggga agcacaccgt taccgaggtt gcatgatcat gggtcagctc
     5641 aaagtcacgc tcgtgaagtc gaagatcggc acgaagccga agcaccgcgg cacggttcgc
     5701 gcgctggggc tgggccgcat cggttcgtcc aacgtcctgc ccgaccgccc ggagatccgt
     5761 ggcatggtc
//
LOCUS       DASUGA010000668         5440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1314153, whole genome shotgun sequence.
ACCESSION   DASUGA010000668 DASUGA010000000
VERSION     DASUGA010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314153"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VFX21_13290"
     CDS             complement(<1..599)
                     /locus_tag="VFX21_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096992.1"
                     /translation="MAEPEVALVFTAEAWVEELHRHLTNHGGARVRQVVVEPTVALEE
                     EYDVLVASHRWPALTRAFVADVHARGRSVLGVYDREEPAAREHLVALGVDQVQECDAG
                     PQALADAVVALPARPLGDGPQHTSEPAPPSAGQTIVVGGPPGSGRTEIAVALAGVLRG
                     ALVDADDVSPSIAARLGLPIEPNLRTAVDALEHGSGSFAA"
     gene            complement(592..1224)
                     /gene="cpaB"
                     /locus_tag="VFX21_13295"
     CDS             complement(592..1224)
                     /gene="cpaB"
                     /locus_tag="VFX21_13295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX5096993.1"
                     /translation="MLQRISTGHIVMLIAGLAGVLLTLHAQRAADDRQAVLVAARDLP
                     PGTVLSEESLRATRVHLPEDALAHVYGARERAELRGRVVTARVEAGALVGRDDVRAAH
                     RGASSRAMSIALPRANAVAGALRAGDRVDVVAVSRDGATADFVMTDTAVLDVDGGDGG
                     PLGAPDAMTITLAVTPESALALARALDAGTVTLVLATDAAPLSIAEPADG"
     gene            1385..1630
                     /locus_tag="VFX21_13300"
     CDS             1385..1630
                     /locus_tag="VFX21_13300"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5096994.1"
                     /translation="MTNPVLPGAEAFPPILTTAQAADLLHVHVEYLRRMVREKRIPAH
                     RFPGGREIRFLRDELIQWVADQPGHDRSPSAEDRADA"
     gene            1627..2493
                     /locus_tag="VFX21_13305"
     CDS             1627..2493
                     /locus_tag="VFX21_13305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5096995.1"
                     /translation="MSLPLEGTRALVTGGNSGIGKETAVALAGMGAQVAIASRNTVKG
                     ENARREIKERAGTDVEVLSLDLASFASVRACAEHYTATHDRLDMLVNNAGIIVRERRV
                     TEDGHEMQFQVNHLGHFLLTSLLRDLLVASAPARVVATASDAHRFVRRGIRFDDLEWE
                     QRRYGIGFACYSTTKLMNILFTRELARTLDATGVTANAVHPGFVASNFAREGDTGRLG
                     NAAMVLGRPFAISNEKGARTSVFVASSPTLNGVTGQYFAKSRFANPTRKARDDDTAAR
                     LWAISAELTDTG"
     gene            2490..3380
                     /locus_tag="VFX21_13310"
     CDS             2490..3380
                     /locus_tag="VFX21_13310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5096996.1"
                     /translation="MKHIGGMDGKTVVITGGNSGIGKETAVALAADGAYVVITARNAG
                     KGEHARREIDERSGRNDCHMVALDLASFASVRTCAQELLDACPRIDVLVLNAGGVLAA
                     RELTEDGHERQLQANHLGHFLLTQLLLDRIVASAPARIVVVASGAHKDAKHGLDWDDL
                     ERARHKYRGFQVYYETKLMNVLFTRELARRLAGTGVTANSVDPGFVASKFAREGDLSW
                     WGNIGMPLTRPFAKSPAKGARTSVYLASSPEVANVTGTYFVQCAAREPSAHGRDDDAP
                     ARLWAISETMVQSAAGDTST"
     gene            complement(3414..3962)
                     /locus_tag="VFX21_13315"
     CDS             complement(3414..3962)
                     /locus_tag="VFX21_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5096997.1"
                     /translation="MTDMDGLAAHPDLAGASAAMREEWRAEQDAAAADAAEQWRHRRT
                     LADRFVEHMHAGDRVAVTVGGHRVTGIPEEAGADLVALRTVFGRVELHIAPGIPLTYE
                     IYEPSAASGGRGNAIAGGQFRNALLHHEHDTEVSVGTLTEPDGLDGRIEVGVDHVKVI
                     ARAGRETVVTLDTITWVSPRRT"
     gene            complement(3982..5115)
                     /locus_tag="VFX21_13320"
     CDS             complement(3982..5115)
                     /locus_tag="VFX21_13320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5096998.1"
                     /translation="MEFGLFQGGFVHKDMAASNPNAEHDRLMSEITLCETADQHNWKY
                     AWFTEHHFLQEYSHISASETLMAWVLARTERIHVASGIINVTPPVNHPARVAERVAMM
                     DLVSNGRFEFGTGRGSSTTEMGGFGITDPEITKDMYDEAIREIVRMWRDEPYSYDGKF
                     FSMPERNVLPKPYVKPHPPLWVAAGNPGTFEKAGQMGLGVICFTGGTPEKMAPLIELY
                     KKNVAKAEPVGEYVNDNVAITTSFMCLEDRDEARDYMAQSGNGRQQTLVFHYLDTFPK
                     PPFVPEWPQEIPDPSLDVIEKGFQSGAAIVGTPDDCAAGIQKWVDVGVDQLIMGPSGS
                     TYPHELLERTVKLFGDEVIPRFDTDPEFSTNRYRKEAAARLGR"
BASE COUNT          846 a         1907 c         1843 g          844 t
ORIGIN      
        1 gcggcgaacg aaccggatcc gtgctccagc gcgtcgaccg cggtgcgcag gttgggttcg
       61 atcggcagcc cgagccgcgc cgcgatcgac ggcgacacgt cgtcggcgtc gacgagcgca
      121 ccgcggagca caccggccag ggcgaccgcg atctccgtgc ggccgctccc cggcggcccg
      181 ccgaccacga tcgtctgccc ggccgacggc ggcgcgggct ccgacgtgtg ctgcgggcca
      241 tcgccgagcg ggcgagccgg cagcgcgacg accgcgtcgg ccagggcttg cggcccggca
      301 tcgcactcct ggacctggtc gacaccgagc gcgacgaggt gctcgcgcgc ggccggttcc
      361 tcccggtcgt agacaccgag caccgaccgg ccacgggcgt ggacgtcggc gacgaacgcg
      421 cgcgtgaggg cgggccagcg atggctcgcg acgagcacgt cgtactcctc ttcgagcgcg
      481 accgtcggtt cgacgacgac ctgccgcacc cgtgcgccac cgtgattcgt gaggtgccgg
      541 tgcaactcct cgacccacgc ctcggccgtg aagaccagcg caacctccgg ctcagccatc
      601 ggccggctcc gcgatcgaga gcggcgccgc gtccgtcgcc agcaccagcg tgaccgttcc
      661 tgcgtcgagc gcgcgtgcca acgcgagcgc ggactccggt gtgaccgcga gtgtgatcgt
      721 catcgcgtcg ggagcaccga gcgggccacc gtcaccgccg tcgacgtcga gtaccgcggt
      781 gtcggtcatg acgaagtccg cggtcgcgcc gtcgcgactg actgcgacga cgtcgacgcg
      841 atcaccggct cgcaacgcgc ccgccaccgc gttcgcgcgc ggcaatgcga tgctcatcgc
      901 gcggctgctc gcgccacggt gggcggcgcg gacgtcgtcg cggccgacga gcgcgccggc
      961 ctcgacgcgt gccgtcacga cgcgcccgcg cagctcggcg cgctctcgcg cgccgtagac
     1021 gtgggcgagg gcgtcttcgg gaaggtgcac ccgcgtggcg cgcaatgact cctcggacag
     1081 cacggtgccg ggcgggaggt cgcgcgcggc caccagcacg gcctggcggt cgtcggccgc
     1141 gcgctgcgcg tgcagggtga gcagcacacc ggccagcccg gcgatcagca tcacgatgtg
     1201 cccggtcgag atccgctgga gcaacggcct gtgtctgatg gtgcaccccc acgcgcccgg
     1261 ctgggcccgg gccatccaac cattgacgaa acggcgtgac aacaggaaag tattgcctga
     1321 gtcttgctag tactgttaca gattgactcg cattgggacg tcggcgcgcc tacgctctcc
     1381 ccccgtgacg aatcccgtgc tgcccggcgc cgaggccttt ccgccgatcc tgacgaccgc
     1441 ccaggccgcg gatctcctcc atgtgcacgt cgagtacctg cggcgcatgg tgcgcgaaaa
     1501 gcgcatcccg gcccaccggt tccccggtgg gcgcgagatc cgcttcctgc gcgacgagct
     1561 gatccagtgg gtggccgacc aacccggcca cgaccgctca ccgtcggccg aggatcgagc
     1621 agacgcgtga gcctcccgct cgagggcacg cgcgcgctcg tcacgggcgg caactcgggc
     1681 atcggcaagg aaacggcagt tgcgctcgcc ggtatgggcg cgcaggtcgc gatcgcgtcg
     1741 cgcaacaccg tgaagggcga gaacgcacgc cgcgagatca aagagcgggc cggcaccgac
     1801 gtcgaggtgc tctcgctcga cctcgcgtcg ttcgcctcgg tgcgcgcgtg tgccgagcac
     1861 tacaccgcga cccacgaccg cctcgacatg ctcgtcaaca acgccggcat catcgtgcgc
     1921 gaacggcgcg tcaccgaaga cggccacgag atgcagttcc aggtcaacca tctcgggcac
     1981 ttcctgctga cgtcgttgct ccgtgacctg ctggtcgcga gcgcgcccgc gcgagtcgtc
     2041 gcgaccgcgt ccgacgcgca ccgcttcgtc cggcgcggca tccgcttcga cgacctcgag
     2101 tgggagcaac gccgctacgg catcgggttc gcgtgctact cgacgacgaa actgatgaac
     2161 atcctcttca cgcgcgagct ggcccgcacg ttggacgcca cgggggtcac ggccaacgcg
     2221 gtgcacccgg gcttcgtcgc cagcaacttc gcacgcgagg gcgataccgg ccgcctcggc
     2281 aacgccgcca tggtgctcgg gcgaccgttc gcgatctcga acgagaaggg cgcccggacg
     2341 tcggtgttcg tcgcgtcctc ccccacgctc aacggcgtga cgggccagta cttcgcgaag
     2401 tcgcgcttcg cgaacccgac caggaaggcg cgcgacgacg acaccgctgc gcgcctctgg
     2461 gcgatcagtg cggagctcac cgacacgggg tgaaacacat cggcggcatg gacggcaaga
     2521 ccgtcgtcat cacgggtggg aactcgggca tcggcaagga gaccgccgtc gccctcgcgg
     2581 ccgacggcgc atacgtcgtc atcaccgccc gcaatgccgg caagggtgag cacgctcgcc
     2641 gggagatcga cgaacgcagc ggccgcaacg actgccacat ggtcgcgctc gacctcgcgt
     2701 cgttcgcgtc ggtgcgcacg tgcgcgcagg agttgctcga tgcctgcccg cgcatcgacg
     2761 tcctcgtctt gaacgcgggc ggcgtgctcg cagcacgcga gctcaccgag gacggccacg
     2821 agcgccagct ccaggcgaac cacctgggac acttcctgct cacacaactg ttgctcgatc
     2881 gcatcgttgc ctcggcgccg gctcgcatcg tcgtcgtggc gtcgggtgca cacaaggacg
     2941 cgaagcacgg cctcgactgg gacgacctcg agcgcgcaag gcacaagtac cgcggcttcc
     3001 aggtctatta cgagaccaag ctgatgaacg tcctgttcac gcgtgagctg gcccgccgct
     3061 tggccgggac gggcgtgacc gccaacagcg tcgatcccgg attcgtcgct tcgaagttcg
     3121 cccgagaagg cgacctgtcg tggtggggca acatcgggat gccgttgacg cggccgttcg
     3181 cgaagtcgcc ggccaagggc gcgcgcacat cggtgtacct cgcctcgtca ccggaggtcg
     3241 cgaacgtcac cgggacctac ttcgtgcagt gcgcggcgcg cgaaccgtcc gcgcacggcc
     3301 gcgacgacga tgcgcccgcg cgcctgtggg cgatcagcga gacgatggtc cagtcggcgg
     3361 cgggcgacac ctcgacctga ggcgcggtgg gcgcgtgccc tgctcgcaac ggtctacgtg
     3421 cggcgcgggc tgacccaggt gatcgtgtcc aaggtcacaa ccgtctcgcg gccggcgcgg
     3481 gcgatcacct tgacgtgatc gacaccgact tcgatgcggc cgtcgagtcc gtcgggctcg
     3541 gtgagtgtgc cgaccgacac ttcggtgtcg tgttcgtggt gcagcaacgc gttgcggaac
     3601 tgaccgccgg cgatggcgtt gccgcggcca ccactcgccg ccgacggctc gtagatctcg
     3661 tacgtcagtg gaatgcccgg cgcgatgtgc agctcgacgc ggccgaagac cgtacgcagg
     3721 gcgacgaggt ccgcaccggc ctcctcaggg atgccggtga cccgatggcc cccgacggtc
     3781 accgcgacgc ggtccccggc gtgcatgtgc tcgacgaagc gatccgcgag cgtgcgccgg
     3841 tgccgccact gctcggccgc gtcggccgcg gccgcgtcct gctccgctcg ccactcctcg
     3901 cgcatcgccg cgctcgcccc cgcgagatcg gggtgcgcag ccagaccatc catgtccgtc
     3961 atgtgcccaa ttgtgacacg attaccggcc gaggcgcgcg gctgcttcct tgcggtagcg
     4021 gttcgtcgag aactcggggt cggtgtcgaa gcgcgggatg acctcgtcgc cgaagagctt
     4081 gaccgtgcgc tcgagcaact cgtgcgggta cgtcgagccc gacgggccca tgatcagctg
     4141 gtcgacaccg acgtcgaccc acttctggat gcccgcggca cagtcgtcgg gcgtaccgac
     4201 gatcgcggca ccgctctgga agcccttctc gatcacgtcg agcgaggggt cggggatctc
     4261 ctggggccac tccggcacga acggcggctt cgggaacgtg tcgaggtagt ggaacacgag
     4321 cgtctgctgg cggccgttgc ccgactgcgc catgtagtcg cgcgcttcgt cgcggtcctc
     4381 gaggcacatg aagctcgtcg tgatcgcgac gttgtcgttc acgtactcac cgaccggctc
     4441 ggccttggcg acgttcttct tgtagagctc gatcagcggc gccatcttct cgggtgtgcc
     4501 gccggtgaag cagatgacac cgaggcccat ctgtcccgcc ttctcgaacg tgccggggtt
     4561 cccggccgcg acccacagcg gcgggtgcgg cttcacgtac ggcttcggca acacgttgcg
     4621 ctcgggcatg ctgaagaact tgccgtcgta cgagtacggc tcgtcgcgcc acatgcgcac
     4681 gatctcgcga atcgcttcgt cgtacatgtc cttggtgatc tcgggatcgg tgatgccgaa
     4741 gccacccatc tcggtcgtcg acgaaccgcg gccggtaccg aactcgaaac gcccgttcga
     4801 gacgaggtcc atcatcgcga cgcgttcggc gacgcgtgcg gggtggttca ccgggggcgt
     4861 gacgttgatg atcccggacg cgacgtggat gcgttcggtg cgcgccagca cccacgccat
     4921 cagcgtctcg ctcgcggaga tgtgcgagta ctcctggagg aagtggtgct ccgtgaacca
     4981 ggcgtacttc cagttgtgct gatcggccgt ctcgcacagc gtgatctcgc tcatgagccg
     5041 gtcgtgttcg gcgttcgggt tggaagcggc catgtccttg tggacgaagc cgccctgaaa
     5101 cagtccgaac tccatggtgc gaggccctcc gggtcagccg gtttttagaa cgtgttacaa
     5161 aaagatagct tcgtggctcc tccgtgtcca gcagtctccg agacacgctt ttcctgctcg
     5221 atgcacgaac atcctgaact acgagagctt gagaaacgtt tttcctgcta tagtgtcccg
     5281 tcgagaaaag gatttcctgc accgtcggga cactcgacaa acggaattcc tgcatgagca
     5341 agctcatctt caccgacgac ccgagcggcg agtggacccg cgctgtacag cgcggcgcgg
     5401 cgcggcgcgt ggcccgcggc acctacacgg aagatctggc
//
LOCUS       DASUGA010000669         3160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_517619, whole genome shotgun sequence.
ACCESSION   DASUGA010000669 DASUGA010000000
VERSION     DASUGA010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517619"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFX21_13325"
     CDS             <1..201
                     /locus_tag="VFX21_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX5096999.1"
                     /translation="VVAAFAGDDRGDLPAFRALSEAVSGERLGAAVRIGVLSPEAPPE
                     LRHMVDLTVDGPDGLADLLGRL"
     gene            complement(336..1685)
                     /locus_tag="VFX21_13330"
     CDS             complement(336..1685)
                     /locus_tag="VFX21_13330"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX5097000.1"
                     /translation="MILVSHRGPFRFTREPDGTFSRGRGAGGVVTALGPLLARDAPVT
                     WIAAAITDDDIAAASSGATSDLGLELHMLDLDEQLHRMHYDVVSNSVLWYVHHDLFDR
                     VHRPQFDDRFREAWDAYVAVNATFADAASDAATEGDIVLVQDYQLALVPELLRARRPD
                     LRVAHFTHTPFAGADALRVLPTDVAERLCESLAGGPAGFHTGRWRDAYRQSAANAPTF
                     VASLGPDAAALQEAAAEPGTRVAADALADVVGERLVVLRTDRIEPSKNIIRGFAAFDR
                     LLEARPGLRGRVVFVAMVYASRQHLADYLAYANEIQHVVARINDRWATRDWTPIVLDE
                     RDDFTRTVAGLQRYDVLFVNPIKDGLNLVAKEGPVLNRRDGIVCLSREAGAFEELRDS
                     VLALHPFDIEQMAVALDDALAMPLDERGARAEAVRNAALSRTPAMWLDDQLQAAVGR"
     gene            complement(1700..2947)
                     /locus_tag="VFX21_13335"
     CDS             complement(1700..2947)
                     /locus_tag="VFX21_13335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5097001.1"
                     /translation="MLERVPLLEKSLDEYAPVVEPGTIERIRELAKPLQGARVLHLNA
                     TAYGGGVAELLMTHVPLTIDAGIDAEWQVIHGSDEFFGVTKSVHNALQGADVEWAPHM
                     ERIFLERTLENAMALEGEYDFIVCHDPQPAALREFVRGRASDAPGTKWIWRCHIDLTD
                     ANDTVWQFYRPYVEQYDAAVFTMPEFVPRSLSMDNVVFAPPTIDPLSVKNLDLAMPFV
                     NEICKSYGVKTNVPLMVQVSRFDPWKDPVGVIEAFRIVREQFPDAELILAGSMATDDP
                     EGFHYWELANEARAGDAAIHLLSNIQQVGSVQVNAFQRAANVVVQKSLREGFGLTVSE
                     ALYKGRPVVGGRCGGITLQVLDGETGYLVDDVESCAKRVVDLLRDPPRADEMGAAGRE
                     HVRQNFCSTRELEDWLRLFNELR"
     gene            2996..>3160
                     /locus_tag="VFX21_13340"
     CDS             2996..>3160
                     /locus_tag="VFX21_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX5097002.1"
                     /translation="MRAVACPDKFRGTLSASDAATAIAAGLTRAGFDEVRTLPLADGG
                     EGTMDALLAAL"
BASE COUNT          485 a         1059 c         1104 g          512 t
ORIGIN      
        1 gttgtcgcgg cgttcgcggg tgacgatcgc ggcgaccttc ccgcgttccg cgcgctcagc
       61 gaagcggtgt cgggcgaacg gctcggcgcg gccgtgcgca tcggcgtgtt gtcacccgaa
      121 gcgccgcccg agctccgtca catggtcgac ctgaccgtcg acggacccga cggcctcgcc
      181 gacctgctcg ggcgtctctg agcaggcggc ggtggcagca gtgtcctccc gatgcgcgaa
      241 gcgccccgac aggaccgtct cggccactcg cggagtcttt cggcgggccg acaacgagac
      301 gtgcctcacg cgagcacgtc gaaagcgaca aagaattatc gcccgaccgc cgcctggagt
      361 tggtcgtcga gccacatggc gggagtgcgg gacagcgccg cgttgcggac ggcttcggcg
      421 cgtgcgccgc gctcgtccag cggcatcgcc aacgcgtcgt cgagggcgac ggccatctgc
      481 tcgatgtcga acgggtgtag tgcgagcacg ctgtcgcgta gctcttcgaa cgcgccggct
      541 tcgcgtgaca agcagacgat gccgtcgcgg cgattcagga ccggtccctc cttcgcgacg
      601 aggttgaggc cgtccttgat cggattcacg aacaagacgt cgtagcgctg gaggcccgcg
      661 accgtgcgcg tgaagtcgtc gcgttcgtcg agcacgatcg gcgtccagtc gcgcgtcgcc
      721 cagcggtcgt tgatgcgggc gacgacgtgc tggatctcgt tcgcatacgc gaggtagtcg
      781 gcgaggtgct gtcgcgatgc gtagaccatc gcgacgaaca ccacgcgccc gcgtaagccg
      841 gggcgtgcct ccagcagccg gtcgaatgcg gcgaagccgc gaatgatgtt cttcgacggt
      901 tcgatccggt cggtgcgcaa gacgacgaga cgttcgccga cgacgtccgc gagggcatcg
      961 gccgcgacgc gggtgccggg ctcggccgcg gcctcctgga gcgccgccgc gtcgggtccg
     1021 aggctggcga cgaacgtcgg ggcgttggcg gccgactgcc ggtatgcgtc ccgccacctc
     1081 cccgtatgga atccggcggg accgcccgcg agcgactcgc acagccgctc ggcgacgtcg
     1141 gtgggcaaca cgcgcagtgc atcggcgccc gcgaacggcg tgtgggtgaa gtgggcgacg
     1201 cggaggtcgg gacgccgggc ccgcaacagt tccgggacga gcgcgagctg atagtcctgc
     1261 accagcacga tgtcgccttc ggtggccgcg tcgctcgcgg cgtcggcgaa cgtcgcgttg
     1321 accgccacgt acgcgtccca cgcctcgcgg aagcgatcgt cgaactgcgg gcgatggacg
     1381 cggtcgaaca ggtcgtggtg cacgtaccaa agcactgagt tcgatacgac gtcgtagtgc
     1441 atgcgatgca gctgctcgtc gaggtcgagc atgtgcagct cgagtccgag atcactggtc
     1501 gcgccgctgc ttgccgccgc gatgtcgtcg tccgtgatcg cggccgcgat ccacgtcacc
     1561 ggtgcgtcgc gcgcgagcag cgggccgagt gccgtgacga cgccgcccgc gccgcggcct
     1621 ctcgagaagg tgccgtccgg ctcgcgcgtg aaccggaacg gtccacgatg cgagacgagg
     1681 atcatgagtg gagcacttgt taccgcaact cgttgaagag tcgcagccag tcttcgagct
     1741 cgcgcgtcga gcagaagttc tggcggacgt gctcgcggcc cgcggcgccc atctcgtccg
     1801 cgcgcggcgg gtcgcgtagc aggtcgacga cgcgcttggc gcacgactcg acgtcgtcga
     1861 cgaggtagcc ggtctcgccg tcgagcactt gcagcgtgat gccaccgcaa cgcccgccga
     1921 cgaccggccg gcccttgtac aacgcctcgc tcaccgtcag accgaagcct tcgcgcagcg
     1981 acttctgcac gacgacgttg gcggcgcgct ggaacgcgtt gacctgcacc gacccgacct
     2041 gctggatgtt cgacagcaag tggattgccg catcaccggc acgcgcctcg ttcgcgagct
     2101 cccagtagtg gaagccctcg gggtcgtcgg tcgccatcga gccggcaagg atgagctccg
     2161 cgtcggggaa ctgctcgcgc acgatacgga acgcctcgat cacgccgaca gggtccttcc
     2221 acggatcgaa acggctcact tgcaccatca gcggcacgtt cgtcttcacg ccgtagctct
     2281 tgcagatctc gttgacgaac ggcatcgcga ggtcgaggtt ctttaccgag agcgggtcga
     2341 tggtcggtgg cgcgaagacg acgttgtcca tcgacaaact gcgagggacg aactccggca
     2401 tcgtgaacac ggccgcgtcg tactgctcga cgtacggccg gtagaactgc cacacggtgt
     2461 cgttggcgtc ggtcaggtcg atgtgacagc gccagatcca cttggtgccc ggcgcgtcgc
     2521 tggcccggcc gcgcacgaac tcgcgcagcg ccgcgggctg cgggtcatgg cacacgatga
     2581 agtcgtactc gccctcgagg gccatcgcgt tctcgagcgt gcgttcgagg aagatccgct
     2641 ccatgtgcgg cgcccactcg acgtccgcgc cttggagcgc gttgtggacc gacttggtga
     2701 cgccgaagaa ctcgtcactg ccgtgaatga cctgccactc ggcgtcgatg ccggcgtcga
     2761 tcgtgagcgg cacgtgcgtc atcagcaact cggcgacgcc gccgccgtac gccgtcgcgt
     2821 tcagatgcag gacccgggca ccctggagcg gtttggccag ctcgcggatg cgttcgatgg
     2881 tccccggttc gacgacgggc gcgtactcgt cgagcgactt ttcgagcaag gggacgcgtt
     2941 cgagcacagg ttctcctggt ggcgggccgt tcggccggtc aatctatgtc acgtgttgcg
     3001 cgccgttgcg tgtcccgaca agttccgcgg aacgctgtcc gcgtccgacg ccgccactgc
     3061 catcgcggcc gggctcacgc gggccggttt cgacgaggtc cggacgctac cgctggccga
     3121 cggcggtgaa ggaacaatgg acgcgctcct cgccgcactc
//
LOCUS       DASUGA010000670         2426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_63960, whole genome shotgun sequence.
ACCESSION   DASUGA010000670 DASUGA010000000
VERSION     DASUGA010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63960"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="VFX21_13345"
     CDS             complement(<1..1090)
                     /locus_tag="VFX21_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003911563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5097003.1"
                     /translation="MTPVRALVLDAPRQLVAREFERPAIGDDDALLRVEACGLCGTDH
                     EQYTGELHPGYGFVPGHEAVGVIEEIGPEAATSWGVARGDRVAVEVFQSCRACDACRA
                     GDYRRCERHGIRDMYGFVPADKAPGLWGGYAEYQYLSPDSMVLPVPAALDPIVATAFN
                     PLGAGIRWAVTVGGAGRGDVVAVLGPGIRGLSACAAAKEAGASFVMVTGRGPRDAARL
                     AIAAEFGADLAVDVAETDPVRALRDATGTPADVIVDVTAKAPAALGQAVALARAGGTI
                     VLAGTRGSNDTPGFAPDNVVYKELHIVGALGVDAPAYRAALDLLASGKYPFADLPREI
                     VTLDEAEPLLQRMAGEGATPPVHGVITPG"
     gene            complement(1087..>2426)
                     /locus_tag="VFX21_13350"
     CDS             complement(1087..>2426)
                     /locus_tag="VFX21_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5097004.1"
                     /translation="EHLALLDGAGAAVPDDRRFVVDTPAWDTALAPFKGAPLPGVAVQ
                     PSDTYMLIFTSGTTGAPKAVKISHGKLTGWGSHLATNFGLSPDDVCYSVMPLFHSNAQ
                     VAGFTNVWFSGATAVLRRRFSASGFLPDVRRYGVTFFNYVGKPLTYILATPAQPDDAD
                     NPLTFAFGNEAAPLDIDRFAQRFGAIVVDGYGSTEGGVTMSRDANTPKGSLGVANNNA
                     DVRVVDPETGEECAPARFDEAGRFLNADTAIGELVNASGAGSFEGYYNNDEANAERMR
                     GGMYWSGDLAYRDEAGYFYFAGRNSDWLRVDGENFAAAPVESVLARHPDVVLAAVYAV
                     PSADVGDEVMAALHWRAGSSFDGAAFAAFITAQPDCSPKWTPRFVRISTALPSTATGK
                     VVKRVLRADGWQCTDEVWWRPGRDTEFRRLTSDDVAALREQFAARGREHLLGR"
BASE COUNT          353 a          837 c          901 g          335 t
ORIGIN      
        1 aacctggggt tatgacgccg tggaccggtg gggtggcgcc ttcgccggcc atgcgttgga
       61 gcaacggctc cgcctcgtcg agcgtgacga tctctcgggg cagatcggcg aacgggtact
      121 tgccgctcgc caacaggtcg agggcggcgc ggtaggcggg ggcgtcgacg ccgagcgcgc
      181 cgacgatgtg cagttccttg tagacgacgt tgtcgggcgc gaaccccggc gtgtcgttgc
      241 tgccgcgcgt ccccgcgagc acgatcgtgc cgcccgcgcg tgccagcgcg accgcttgac
      301 cgagcgcggc cggtgccttg gcggtgacgt cgacgatcac gtcggccggc gtgcccgtcg
      361 cgtcgcgcaa ggcgcgcacg ggatcggtct cggcgacgtc gaccgcgaga tccgcaccga
      421 actcggctgc gatcgcgaga cgcgcggcgt cgcgcgggcc gcgtccggtg accatgacga
      481 acgacgcgcc tgcctccttg gcggccgcgc acgcactgag gccgcggatg cccggaccga
      541 gcacggccac gacgtcgccg cggcccgcgc cgccgacggt gaccgcccag cggatgccgg
      601 cgccgagcgg gttgaaggcg gtcgcgacga tcgggtcgag cgcggcgggg acgggcagca
      661 ccatcgagtc gggggagagg tactggtact cggcgtagcc gccccagagg cccggtgctt
      721 tgtcggcggg cacgaagccg tacatgtcgc ggatgccgtg gcgctcgcac cggcggtagt
      781 cgccggcgcg gcacgcgtcg caggcgcggc acgactggaa gacctcgacc gcgacgcgat
      841 caccgcgtgc cacgccccaa ctcgtcgcag cctcgggtcc gatctcctcg atgacgccga
      901 ccgcttcgtg gccgggcacg aacccgtagc cgggatgcag ttcacccgtg tactgctcgt
      961 ggtcggtgcc gcacaggccg cacgcctcga cccgcagcag cgcgtcgtcg tcaccgatcg
     1021 ccggtcgctc gaactcgcgc gcgaccaact gccggggcgc gtcgagcacg agcgcgcgta
     1081 cgggcgtcac cggccgagga ggtgctcgcg gccacgcgcc gcgaactgct cgcgcagcgc
     1141 ggcaacgtcg tcactcgtca ggcggcggaa ctccgtgtcg cggccggggc gccaccacac
     1201 ctcgtcggtg cactgccaac cgtccgcgcg caacacgcgc ttgacgacct tccccgtcgc
     1261 ggtcgacggc aacgccgtcg agatgcgcac gaagcgcggg gtccacttcg gtgaacagtc
     1321 gggttgcgcg gtgatgaacg cggcgaacgc ggcaccgtcg aacgacgaac ccgcgcgcca
     1381 gtgcaacgcg gccatcactt cgtcaccgac gtcggcggac gggacggcat agacggcggc
     1441 cagcacgacg tcggggtgcc gggcgagcac cgactcgacg ggcgcggccg cgaagttctc
     1501 gccgtcgacg cgcagccagt cggagttgcg gcccgcgaag tagaagtagc cggcttcgtc
     1561 gcggtacgcg aggtcgcccg accagtacat gccgcctcgc atgcgctcgg cgttcgcttc
     1621 gtcgttgttg tagtagccct cgaacgagcc cgcgccggac gcattgacca gttcgccgat
     1681 ggcggtgtcg gcattcagga agcggcccgc ctcgtcgaag cgggcgggtg cacactcttc
     1741 gccggtctcg gggtcgacga cccgcacgtc ggcgttgttg ttcgcgacgc cgagcgagcc
     1801 cttcggcgtg ttggcgtcgc ggctcatcgt gacgccgccc tcggtggagc cgtagccgtc
     1861 gacgacgatc gcgccgaagc gctgcgcgaa gcggtcgatg tcgagcggcg cggcttcgtt
     1921 gccgaacgcg aaggtcaacg ggttgtcggc gtcgtcgggt tgggccggcg tcgcgaggat
     1981 gtacgtgagc ggcttgccga cgtagttgaa gaaagtcacg ccgtagcggc gcacgtcggg
     2041 caggaaaccg ctcgcggaga accggcgccg caacaccgcg gtcgcgccgg agaaccagac
     2101 gttggtgaac ccggcgacct gcgcgttcga atggaacagc ggcatcaccg agtagcagac
     2161 gtcgtccggt gagagcccga agttggtggc gaggtgcgaa ccccatcccg tgagcttgcc
     2221 gtggctgatc ttcaccgcct tcggcgcgcc ggtcgtgccc gacgtgaaga tcagcatgta
     2281 cgtgtcggac ggctgcaccg cgacgccggg gagcggcgcg cccttgaacg gcgcgagcgc
     2341 ggtgtcccac gcgggcgtgt cgacgacgaa ccggcggtca tccggtacgg cggccccggc
     2401 accgtcgagg agcgcgaggt gctcgg
//
LOCUS       DASUGA010000671         2806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1453315, whole genome shotgun sequence.
ACCESSION   DASUGA010000671 DASUGA010000000
VERSION     DASUGA010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453315"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..232)
                     /locus_tag="VFX21_13355"
     CDS             complement(65..232)
                     /locus_tag="VFX21_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097005.1"
                     /translation="MMFLLFVVVASAIGISIVLMRNRRPTSMEHSIDEFERGLRALAP
                     EFPTRRSRPQE"
     gene            complement(229..738)
                     /locus_tag="VFX21_13360"
     CDS             complement(229..738)
                     /locus_tag="VFX21_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX5097006.1"
                     /translation="MFDAPPDPAAHDWVALGPAPLPTDAAVAWATTPGAGAVVSFLGV
                     VRDHADGRDGVHSMTYEAYEEPAVRAMTDIAAAARRQWPDLCRLALLHRVGELRLSEA
                     SVLVVASSAHRAVAFDAARYCIDTLKESVPIWKQEHWAGGSSWAVEQHPIRSVDEQAS
                     AGKVSGVSR"
     gene            821..1984
                     /locus_tag="VFX21_13365"
     CDS             821..1984
                     /locus_tag="VFX21_13365"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEX5097007.1"
                     /translation="MAPAGDEHHEGHDDDFGDWGDEAPRGAPPSRDDRLWVHPAELTG
                     TPRPKPRRRLAWVAPLSTGVLGAVAAVSVLGMAGAFDDNSEPKDAPAAAASTVAEENA
                     PQEAAALVAPSLVVLTVRDDKGARRAAGVCVRRNGDILTSARFVDGVQSAEVTTADGT
                     TTGARVLATDTATDIALLSVGEALEAPPMANEKPRPGDPVWLVGPPVKEDAEPWMSSG
                     VVTADDALVADEPGPTIGGLLQTDASYSAQASGGALVDSDGKLTGIVLGPVGDNGVTY
                     AMPIANAMQIARALRTDGHVPHGSLGLVGIDTAQGPTVTEVAGGGSAAKAGLQPGDVI
                     LIVAGRPVIDMAEVTATVRTYAPGRTVEIEVQRGDDRLNYDVMLTEKTAEAAD"
     gene            2009..>2806
                     /locus_tag="VFX21_13370"
     CDS             2009..>2806
                     /locus_tag="VFX21_13370"
                     /inference="COORDINATES: protein motif:HMM:NF037131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HEX5097008.1"
                     /translation="MTEELEELDRELLNAVQWDFPLEPRPFAVLGERLGMSEPEVSDR
                     IRKVTDAGVLRQLSAIFDTRALGYTSALVAAKVDPDRIDEAAEIISAHPGVSHNYKRN
                     HEYNLWYTVAVPPGESLDEHVDVLHEASGSRVTRKLPTLKLYKIGVKLDMTGKMAANA
                     KAEVLEHERPERKAEMPAPDLSDLEVRAIRVVQEPLPIVERPFAAYGAQIGADVTEDD
                     VLALLRSFKERKLMRRFAAVMNHRSAGFKANAMGVWAVPEDMLETIGP"
BASE COUNT          469 a          979 c          996 g          362 t
ORIGIN      
        1 cgtacacgag cgtgttcgcg gtaccgagat cgatggcaag gtcacgaggc agcgtgcacg
       61 cctctcactc ctggggccgc gaacgccgcg tcgggaactc cggcgccagc gcgcgcaacc
      121 cgcgctcgaa ttcgtcgatg ctgtgctcca tcgacgtcgg ccgccggttg cgcatcagca
      181 cgatcgagat gccgattgcg ctcgcgacca cgacgaacag caggaacatc atctcgagac
      241 tcccgagacc ttccccgcgg atgcttgctc gtcgaccgaa cggatcgggt gttgctcgac
      301 cgcccaactg gagccacccg cccagtgctc ttgcttccag atcggcaccg actccttcag
      361 cgtgtcgatg cagtagcgcg cggcgtcgaa cgcgacggcg cggtgcgcgg acgatgcgac
      421 gacgagcacc gaagcttccg acaagcgcag ctcaccgacg cggtgcaaga gcgcgagccg
      481 gcagaggtcg ggccactggc gacgcgcggc ggctgcgatg tcggtcatcg cgcgcacggc
      541 cggttcctcg taggcctcgt acgtcatgga gtggacgccg tcacgaccgt cggcgtggtc
      601 gcgaaccacc cccaggaacg acaccacggc gcccgccccg ggtgtggtgg cccaggcgac
      661 cgcggcatcg gtcggcaacg gcgcgggacc gagcgcgacc cagtcgtgcg cagcgggatc
      721 cggcggggcg tcgaacatcg gtggcagtct acgaaagacc cctggtcggg gcccggggag
      781 gcgcggtcgc gagtgcacgg cccgtcgtta cactcgccga atggctcccg cgggtgacga
      841 gcaccacgag ggccacgacg acgatttcgg cgattgggga gacgaagcgc cgcgtggcgc
      901 tccaccgagc cgcgacgacc gtctctgggt gcatccggcc gagctcacgg gaactccgcg
      961 gcccaagccc cggcggcggc tggcctgggt cgcgccgctg tcgacgggcg tgctcggcgc
     1021 cgtcgcagcc gtctcggtcc tcggcatggc cggtgcgttc gacgacaatt ccgagccgaa
     1081 ggacgcgccc gcggcggccg catcgaccgt cgccgaggag aacgctccgc aggaggcggc
     1141 cgcgctcgtc gcgcccagtc tcgtcgtcct caccgttcgc gacgacaaag gtgcgcggcg
     1201 cgcggccggc gtctgcgtcc gccgcaacgg cgacatcctg accagcgcgc ggttcgtcga
     1261 cggcgtgcag tccgcggaag tcacgaccgc ggacgggacg acgaccggcg cccgcgtcct
     1321 cgcgaccgac acggccaccg acatcgcctt gctctcggtc ggcgaagcgc tcgaggcgcc
     1381 gccgatggcg aacgagaagc cgcgcccggg cgaccccgtg tggctcgtcg gaccgcccgt
     1441 caaagaggac gccgaaccgt ggatgagcag cggcgtcgtc acggccgacg acgcgttggt
     1501 ggccgacgaa cccggaccga cgatcggcgg gctgctccaa acggacgcgt cgtacagcgc
     1561 gcaggcgagc ggcggtgcgc tcgtcgacag cgacggcaag ctcaccggca tcgtgctcgg
     1621 tccggtcggc gacaacggcg tgacgtacgc gatgccgatc gccaacgcga tgcagatcgc
     1681 gcgcgcgttg cgcaccgacg gccatgtgcc acacggctcg ctcggcctcg tgggcatcga
     1741 caccgcgcag ggaccgacgg tcaccgaggt ggcgggcggc ggatcggccg cgaaggccgg
     1801 gctccaaccc ggtgacgtca tcctcatcgt ggcaggacgg ccggtcatcg acatggccga
     1861 agtcacggcg accgttcgca cctacgcgcc gggccgcacc gtcgagatcg aggtgcaacg
     1921 gggcgacgac cgcctcaatt acgacgtcat gctgacggag aagaccgccg aggcggcgga
     1981 ctgaccccga gcgcgcgacg atggaaccgt gacggaagaa ctcgaggagc tcgaccgcga
     2041 gctgctcaat gcagtgcagt gggacttccc gctcgagccc cggcccttcg cggtgctcgg
     2101 cgagcgcctc ggcatgtccg aaccggaggt gagcgaccgc atccggaagg tgaccgacgc
     2161 cggcgtgctg cgccagctgt cggcgatctt cgacacccgt gcgctcgggt acacgtcggc
     2221 actcgtcgcg gcgaaggtcg accccgaccg tatcgacgag gcggccgaga tcatcagcgc
     2281 gcatccgggc gtgtcgcaca actacaagcg caaccacgag tacaacctct ggtacacggt
     2341 cgcggtgccg cccggtgagt cgctcgacga gcacgtcgac gtgttgcacg aggcgtcggg
     2401 ttcgcgcgtc acgcgcaagc tgccgacgct caagctgtac aagatcggcg tgaagctcga
     2461 catgaccggc aagatggccg cgaacgcgaa ggccgaggtg ctcgagcacg aacgtccgga
     2521 gcgcaaggcc gagatgcccg cgcccgacct cagcgatctc gaagtgcgcg cgatccgcgt
     2581 cgtgcaggag ccgctgccga tcgtggaacg gccgttcgcg gcgtacggcg cgcagatcgg
     2641 cgccgacgtc accgaagacg acgtgctcgc gttgttgcgc tcgttcaagg aacgcaagct
     2701 catgcgccgc ttcgcggcgg tgatgaacca ccgcagcgcg ggcttcaagg cgaacgcgat
     2761 gggcgtgtgg gccgtgccgg aggacatgct cgagacgatc ggcccg
//
LOCUS       DASUGA010000672         2246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_898935, whole genome shotgun sequence.
ACCESSION   DASUGA010000672 DASUGA010000000
VERSION     DASUGA010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898935"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VFX21_13375"
     CDS             complement(<1..876)
                     /locus_tag="VFX21_13375"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5097009.1"
                     /translation="MTRRHSPALLRVRWSWRDLRARWLQVAAIALIIGIGSGTYSGLS
                     SVSAWRHSSYDASYAASNMYDLRYEFATGSFVDAEQLRTALATTAAAGDVQAISPRLV
                     QATQVDASTEDPPVLVPGRLVGVDVREGGPHVNAVRVFSGRALTEADAGAPVAVLDEH
                     FADKHGIRAGVELRTGGGSTFGIVGRGLGPDDFLVIGDQGSLWADFAVLYAPLATVQA
                     ATGHEGQVNDVLITVRPGAERGAVEAQVSAALDGAFPDAGYTVTHQEDDRALRLLYDD
                     IDGDQRFYNIFAVLIL"
     gene            complement(873..1541)
                     /locus_tag="VFX21_13380"
     CDS             complement(873..1541)
                     /locus_tag="VFX21_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018502489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5097010.1"
                     /translation="MGDTTVEALRAVDLSIDRGEFCVVLGPSGSGKTTLLNLVGALDT
                     PTSGEVNVAGIKTSGASRRELFRFRRETVSFVFQTFNLFPALTARENVQFAIDVTHRN
                     HDGDTAAEVLARVGLADRLDHFPHQLSGGEQQRVAIARALATGNPVLLADEPTGELDF
                     HTGVQILELLHEQAHAGVTVLIVTHNREISRVADRVIELSSGAIEGDGPPPGGPVDIG
                     SLRW"
     gene            1717..>2246
                     /locus_tag="VFX21_13385"
     CDS             1717..>2246
                     /locus_tag="VFX21_13385"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5097011.1"
                     /translation="MTGTAAPGALGETSAGHGRHFWRRAFVAFAAVLVVVTSVVVVQY
                     RRQIVSYVTHLKGSPTHTTAYERFDPAPEFHLAAAGDIGEPGGRLDATGRTMAEIGAT
                     TPYDALLLLGDNVYPNGDPAKLPRTVFEPFAPVLDQDTELLAILGNHDVKDGNGPPQM
                     EALGMPGRYWSKRYGDV"
BASE COUNT          365 a          794 c          762 g          325 t
ORIGIN      
        1 gaggatgagc acggcaaaga tgttgtagaa gcgctggtca ccgtcgatgt cgtcgtagag
       61 cagccgcagg gcgcggtcgt cctcttggtg cgtcacggtg taaccggcat ccgggaacgc
      121 gccgtcgagc gcggcggaga cctgcgcttc gaccgcaccc cgttccgcgc ccggccggac
      181 ggtgatgagc acgtcgttga cctggccctc gtgaccggtc gcggcctgca cggtcgcgag
      241 cggcgcatag agaactgcga agtcggccca cagacttccc tggtcaccga tcacgaggaa
      301 gtcgtcggga cccagcccgc gcccgacaat gccgaacgta ctccctccgc cggtgcgcag
      361 ctccacgccg gcgcggatcc cgtgcttgtc ggcgaaatgt tcgtcgagca ccgcgaccgg
      421 cgcgccggcg tccgcctcgg tgagtgcgcg gcccgagaac acgcgcacgg cgttgacgtg
      481 cgggccgccc tcgcgcacgt cgacgccgac caaccggccc ggaacgagga ccggcgggtc
      541 ctcggtgctc gcgtccacct gcgtcgcctg cacgagccgg gggctgatcg cctgcacgtc
      601 gccggccgcc gcggtggtgg cgagcgcggt gcgcaattgc tcggcgtcga cgaagctccc
      661 ggtcgcgaac tcgtaccgca agtcgtacat gttcgaggcc gcgtacgagg cgtcgtacga
      721 gctgtgacgc caggccgaga cgctcgagag ccctgagtac gttcccgacc cgataccgat
      781 gatgagcgcg atggcggcca cctgcagcca acgggcacga aggtcgcgcc acgaccagcg
      841 cacgcgcaac aaggcgggac tgtgacggcg cgtcaccagc gcaggcttcc gatgtcgacg
      901 ggaccgcccg gcggcggacc gtcgccctcg atggcaccgc tcgacagctc gatcacgcgg
      961 tccgcgacgc ggctgatctc gcggttgtgc gtcacgatca acaccgtcac gccggcgtgg
     1021 gcctgttcgt gcaacagctc gaggatctgc acacccgtgt ggaagtccag ctccccggtc
     1081 ggctcgtcgg ccaacagcac cgggttgccg gttgcgagcg cgcgtgcgat cgcgacccgc
     1141 tgttgctccc caccggagag ctggtgcggg aagtgatcca gacgatcggc caaaccgacg
     1201 cgggccagca cctcggccgc agtgtcaccg tcgtgattgc ggtgcgtcac gtcgatcgcg
     1261 aactggacgt tctcgcgtgc ggtcaaagcc gggaacaggt tgaacgtctg gaagacgaag
     1321 ctcaccgtct cgcgccggaa gcgaaacagt tcgcgccggc tcgcgcccga ggtcttgatc
     1381 cccgcgacgt tgacttctcc cgacgtcggc gtgtccaacg cgccgacaag gttgagcaac
     1441 gtcgtcttcc cgctgcccga cggaccgagc acaacgcaga actcaccgcg atcgatcgac
     1501 agatcgacgg cgcgcagcgc ctccacggtg gtgtcgccca cgtgatagcg acgcccggcg
     1561 cctgaaagct cgatgacggc gatgacgcga cctccggtcc acgacggtgc gcccagcgtc
     1621 gcaacgctcc ggcgcgaccg aaagggcctt tcggcccgac tcgatcggga ccgaggccgc
     1681 tcacggcggc gccccgttcg ggcgcacgat cgagtcatga cggggaccgc cgctcccggc
     1741 gcgttgggcg agacgagtgc cggtcatggc cggcacttct ggcggcgcgc gttcgtcgca
     1801 ttcgcggccg tgctggtcgt ggtgacctcg gttgtcgtcg tgcagtaccg ccgtcagatc
     1861 gtgtcgtacg tgacgcacct caagggcagt ccgacgcaca ccaccgcgta cgaacgcttc
     1921 gaccccgcgc ccgagttcca cctcgcggcg gccggcgaca tcggcgagcc cggcggacga
     1981 ctggacgcca ccggccgcac gatggccgag atcggcgcga cgacgccgta cgacgcgctg
     2041 ttgctcctcg gcgacaacgt ctatccgaac ggcgatcccg cgaagttgcc ccggacggtg
     2101 ttcgaaccgt tcgcgcccgt cctggaccag gacaccgagt tgctcgccat cctcggcaat
     2161 cacgacgtca aggacggcaa cggacccccg cagatggaag cactggggat gccgggtcgc
     2221 tactggtcga agcgctacgg cgacgt
//
LOCUS       DASUGA010000673         2758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_899440, whole genome shotgun sequence.
ACCESSION   DASUGA010000673 DASUGA010000000
VERSION     DASUGA010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899440"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VFX21_13390"
     CDS             complement(<1..656)
                     /locus_tag="VFX21_13390"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5097012.1"
                     /translation="MTATDPTIASGLSGLRALQHPAKVLLARRLVAAGKVPQVADFDK
                     QGLRLTDDEGKALQRWLDVGLVTRVVRESRSRDGTVKLLLELRDGKKVETVAMPVGAC
                     CVSTQVGCAVGCRFCASGLFGVERHLSVDEILEQIVHCRRQMRIDRVVFMGMGEPTHN
                     LDAVMAAVARIKDEVLISPRRQTLSTVGGVRVLERMRKEFAVRPCLAWSLHVANEAKR
                     A"
     gene            complement(717..2312)
                     /locus_tag="VFX21_13395"
     CDS             complement(717..2312)
                     /locus_tag="VFX21_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097013.1"
                     /translation="MANATAPFHPIIYVRGFAMSRGEIDETTADPFCGFNLGSTVYRA
                     VPDKSRPPRKFVFESPVVRLASDFGYRDVYEDGYDLLDPEWDDDADHEVGNRCIVVHR
                     YYDEASSLLGTSETPPIETFAKKLGDLILRLRDLVCGNAENGIAPADFRCYLVAHSMG
                     GLVCRAFLQNTKNDPKDAARFVDKLFTYATPHNGIDVAGFNVPKLIDVGSTKNFNRDE
                     MARYLALGKVYERTERVDWIPEDRFPASRVFTMVGTNRNDYEAAMGLSRAFVGHGSDG
                     LVRIENATLTGLRADGKPGDCCAKAFTYRAHSGYFGIVNSEEAYQNLTRFLFGDVRAD
                     VFVDFDEFRVPAEIEADDAKGKVNALYQIELLASPRGKMWYLTRRVAEEDSVACLSHA
                     EWKQDPKKKGTHYLSTVFLAKRARVDPKRKSLAYQVTIGIRVPDWEVDRALWFDKHYE
                     GGYLFRDALLIELEPPTKTGGWKVKHAWQGTAVNQQMTTVPVQGLDKGRVEVVVPFDS
                     KATPGVAGALRFVLSAWNPGGAQ"
     gene            2414..>2758
                     /locus_tag="VFX21_13400"
     CDS             2414..>2758
                     /locus_tag="VFX21_13400"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEX5097014.1"
                     /translation="MTQPPTARSHTMPVVFVSHGAPTLAIDPVAGADFTRLGASLPPP
                     RAVLVVSAHWLDTPPAIGTRTRVPLLHDYAGFPETLYRIRYDAPAAGDLADELQRRLP
                     SLERADERPWDHG"
BASE COUNT          400 a         1005 c          916 g          437 t
ORIGIN      
        1 gcgcgcttcg cttcgttcgc gacatgcagc gaccacgcga ggcacggccg cacggcgaac
       61 tccttgcgca tgcgctcgag cacgcgcacg ccgccgaccg tcgacagcgt ctgccggcgc
      121 ggcgagatca gcacctcgtc cttgatgcgc gcgacggctg ccatcacggc gtcgaggttg
      181 tgcgtcggct cgcccatgcc catgaacacg acgcggtcga tccgcatctg gcgccggcag
      241 tgcacgatct gctcgaggat ctcgtcgacg ctcaggtgcc gctcgacgcc gaacagcccc
      301 gacgcgcaga agcggcagcc gaccgcgcaa ccgacctgcg tcgacacgca gcacgcaccg
      361 accggcatcg cgaccgtctc gaccttcttg ccgtcgcgca gctcgagtag cagcttgacc
      421 gtgccgtcgc gcgaacggga ctcgcgcacg acgcgcgtga cgagcccgac gtcgagccag
      481 cgctgcagcg ccttcccctc gtcgtcggtc agccgcagcc cctgcttgtc gaagtccgcg
      541 acctgcggca ccttccccgc cgcgacgagg cgccgcgcga gcagcacctt cgcgggatgc
      601 tgcagcgcgc gcaggccgga caggccggac gcgatcgtcg ggtcggtggc ggtcatcggg
      661 cgcggcatcg tctccgcgac ggtcccggat gccacccctc cgtcacgcgg cgcagctcat
      721 tgcgcgccgc ccgggttcca tgcgctcagc acgaagcgca gtgcgccggc gacgccgggc
      781 gtcgccttgc tgtcgaaggg cacgacgacc tcgacgcggc ccttgtcgag gccctgcacc
      841 ggaacggtcg tcatctgctg gttgaccgcg gtgccctgcc acgcgtgctt caccttccag
      901 ccgccggtct tcgtcggcgg ctcgagctcg atcagcagcg cgtcgcggaa caggtagccg
      961 ccctcgtagt gcttgtcgaa ccacaacgcg cgatcgacct cccagtccgg cacgcggatg
     1021 ccgatcgtca cctggtacgc gagcgacttg cgtttcggat cgacgcgtgc gcgtttggcg
     1081 aggaacaccg tcgacaggta gtgcgtcccc ttcttcttcg ggtcctgctt ccactcggcg
     1141 tgcgacagac aggcgaccga gtcctcctcg gcgacgcggc gcgtcaggta ccacatcttg
     1201 ccgcgcggcg acgcgagcag ctcgatctgg tacagcgcgt tgaccttgcc cttcgcgtcg
     1261 tccgcctcga tctccgcggg cactcggaac tcgtcgaagt cgacgaacac gtcggcgcgc
     1321 acgtcgccga acaggaagcg cgtcaggttc tggtaggcct cctcgctgtt gacgatgccg
     1381 aagtagccgg agtgcgcgcg atacgtgaac gccttcgcgc agcagtcgcc cggcttgccg
     1441 tcggcgcgca gcccggtcag cgtcgcgttc tcgatgcgca cgaggccgtc gctgccgtga
     1501 ccgacgaacg cgcgcgacag gcccatcgcg gcctcgtagt cgttgcgatt cgtgccgacc
     1561 atcgtgaaca cgcgcgacgc cgggaagcgg tcctccggga tccagtcgac gcgctcggtt
     1621 cgctcgtaga ccttgccgag cgcgagatag cgcgccatct cgtcgcggtt gaagttcttg
     1681 gtgctgccga cgtcgatcag cttcggcacg ttgaagcccg cgacgtcgat gccgttgtgc
     1741 ggcgtcgcgt acgtgaacag cttgtcgacg aagcgggcgg cgtccttcgg gtcgttcttc
     1801 gtgttctgca ggaaggcgcg gcagacgagc ccgcccatcg agtgcgcgac gaggtagcag
     1861 cggaagtcgg cgggcgcgat gccgttctcg gcgttgccgc acacgaggtc gcgcaggcgc
     1921 aggatcaggt cgccgagctt cttcgcgaac gtctcgatcg gcggcgtctc ggacgtcccg
     1981 agcagcgacg acgcctcgtc gtagtagcga tgcacgacga tgcagcggtt gccgacctcg
     2041 tggtccgcgt cgtcgtccca ctccggatcg aggaggtcgt agccgtcctc gtagacgtcg
     2101 cgatagccga agtcggacgc gaggcgcacg accggcgact cgaacacgaa cttgcgcggc
     2161 ggccggctct tgtcgggcac cgcgcgatag acggtcgacc cgaggttgaa gccgcagaac
     2221 gggtccgcgg tcgtctcgtc gatctcgccg cggctcattg cgaagccacg cacgtagatg
     2281 atcgggtgga aaggagcggt cgcgttcgcc atcggcattt cccctgcaga gagcggcaca
     2341 tcctatggcc gcggcgcgtc agaccaccac aacctctctc accggctcac gcactggctc
     2401 gtctcctgcc ccgatgaccc agccccctac ggctcgttcg cacacgatgc ccgtggtgtt
     2461 cgtgtcgcac ggcgccccaa ccctggcgat cgacccggtc gccggcgccg acttcacgcg
     2521 cctcggcgca tcgctccccc cgccgcgcgc ggtgctcgtc gtgtcggcgc actggctcga
     2581 cacgccgccg gcgatcggca cgcgcacgcg ggtcccgctg ctgcacgact acgcgggctt
     2641 cccggagacg ctgtaccgca tccgctacga cgcgcccgcg gccggcgatc tcgcggacga
     2701 gctgcagcgc cggctgccgt cgctcgaacg cgcggacgag cgaccgtggg accacggc
//
LOCUS       DASUGA010000674         2986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_205686, whole genome shotgun sequence.
ACCESSION   DASUGA010000674 DASUGA010000000
VERSION     DASUGA010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205686"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VFX21_13405"
     CDS             complement(<1..563)
                     /locus_tag="VFX21_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097015.1"
                     /translation="MDQANDRGDTQVNLKTRLLSGASVLAIAGGVFVAAAPAAPAEVT
                     QVGACEGQALLATFYNSANALAPLTDVTQKGVTVKTKLLSDQTTKAKIAGDCDTATRP
                     GEPGYDANGEIAALTPKAVAGKLVGNASCYTTDLGGDPNEADAWPLSGKIVFTGTQLN
                     DLGKPVQIQAQITVLGFDPVIADAVNVG"
     gene            902..1849
                     /locus_tag="VFX21_13410"
     CDS             902..1849
                     /locus_tag="VFX21_13410"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX5097016.1"
                     /translation="MKETFPTDRVRPRRRITGISAVLVPYTEDREIDWAAFEAHVERT
                     HAAGLVPAVNMDTGYVQLLDENDRTAALECAAKVTSRNFVAGAYVADRPGTPVDLDGY
                     RRRCEEITGLGGTPVIFPSHGLNALDDEEWVAGLATIGDAVDRFIGFELGPMFVPYGR
                     IVSLDAYAAMMEIASCVGAKHSSLNRAAEWERLALRDRLRPDFAVYTGNDLAIDMVMY
                     GSDYLLGVSTFAPDLFAARDAMWEAGESEFFELNDLLQYLGQFAFRPPVPAYRHDAAM
                     FLELRGWARTDATPPGVERRPASDRAVLEDILQRFEAWT"
     gene            1846..>2986
                     /locus_tag="VFX21_13415"
     CDS             1846..>2986
                     /locus_tag="VFX21_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HEX5097017.1"
                     /translation="MRLTQVKNLKDLHAFKAHVDALAIDVPVDDVVAPDGVLGEPVSF
                     TDGTAGTLQSPNRFAILPMEGWDGSPEGRPTDLVRRRWERFGRSGCGLVWAEATAVRA
                     DGRANPSQLLLDDTTLDDFAALRALLDPSQVAGLQLTHSGRYACAQPRIAYRHPILDA
                     RVGADDANLLSDDELDELVACYVDAAVRARTAGFDFVDIKHCHGYLLHELLSARDRPG
                     RFGGDFEGRTRFLSSVVEGIRSRAPDLAIAVRLSAFDFVPFVAGDDNRGTPEWHGPYP
                     HAFGGDGSGTGIDLREPHLLLDLLASMGVRLVSTTAGSPYYVPHVQRPAYFPPSDGYQ
                     PPEDPLVGVARQIHATSEIKRRHPNLIIVGSAYSYLQEWLPYVGQA"
BASE COUNT          499 a          986 c          967 g          534 t
ORIGIN      
        1 ccgacgttaa cggcgtcggc aatcaccggg tcgaagccca gcaccgtgat ctgcgcctgg
       61 atctgcactg gcttgccgag gtcgttgagc tgggtgccgg tgaagacgat cttgccgctc
      121 aacggccagg cgtccgcctc gttcgggtca ccaccgaggt cggtggtgta gcagctcgcg
      181 ttaccgacga gcttgcccgc cactgccttc ggcgtcagcg ctgcaatctc accgttggcg
      241 tcgtaaccgg gctcaccggg acgggtcgcg gtgtcgcagt cgccggcgat cttcgccttg
      301 gtcgtctggt ccgacaagag cttcgtcttg acggtgacgc ccttctgggt cacgtcggtc
      361 aacggcgcga gcgcgttggc cgagttgtag aaggtggcca agagggcctg accctcacac
      421 gcacctacct gggtcacctc tgcgggtgcc gcgggtgcgg cagccacgaa aacgccgccc
      481 gcgatcgcca gcacgctggc gcccgagagc aagcgggttt tcagattcac ctgtgtgtct
      541 cctctgtcat ttgcttggtc caagcgggcc gaccgcacag cccttcaatg acgtcagaag
      601 gcgtgatgac gaacgagatg cgcaacgggc actcccccaa ccccatcgtt tccccctaac
      661 ccctccggtg tgcaacaagt cgggcaatcg ggcgacgctc gccttgcaac attccggatg
      721 cggtccgcaa gaaggttggt ggtactggca gaatttgtca aggagccctg tggacaccta
      781 gaggcgattt gtccccaaag cgcaacccgt gaaaagcacc gcgcaaataa aacgaagagt
      841 acgtgcgtgt tcgggacaac gactttgcta gccgttcggt ccggtagtga ggacacacat
      901 catgaaagag acgttcccca ccgaccgcgt gcgaccccgc cgccgcatca ccgggatctc
      961 ggcggtgctc gtcccgtaca ccgaggacag ggagatcgac tgggctgcgt tcgaagcgca
     1021 cgtcgaacga acgcacgcgg ccgggctcgt gcctgccgtc aacatggaca ccggctacgt
     1081 gcaactgctg gacgagaacg accgaacggc ggcgctcgag tgcgccgcaa aggtcaccag
     1141 ccgaaacttc gtggcggggg cgtacgtcgc tgaccgtccc ggtacgccgg ttgatctcga
     1201 cggctaccgg cgccgctgcg aagagatcac tggtctcggc ggcacgccgg tgatcttccc
     1261 ctcgcacgga ctcaacgcgc tcgatgacga ggaatgggtc gccggcctcg cgacgatcgg
     1321 agacgcagtc gaccggttca tcgggttcga actcggtcct atgtttgtgc cgtacggaag
     1381 gatcgtgtcg ctcgacgcgt acgcggcgat gatggagatc gcctcctgtg tgggtgcgaa
     1441 gcattcgtcg ctcaaccgcg ccgctgagtg ggagcgactc gcgctgcgcg accggctccg
     1501 gccggacttc gccgtgtaca ccggcaacga tctcgcgatc gacatggtca tgtacggctc
     1561 ggactatctg ctcggcgtgt cgacgttcgc gcccgatctc ttcgccgcgc gcgatgcaat
     1621 gtgggaagcc ggcgagtccg agttcttcga gctcaacgat ctgttgcagt acctcggtca
     1681 gttcgcgttc cggccgccgg tgccggccta ccgccacgat gccgccatgt tcctcgaact
     1741 gcgcgggtgg gcgcgcaccg acgcgacgcc gcccggggtt gagcgcaggc cggcgtcgga
     1801 tcgcgcggtg ctggaagaca tcctgcagcg cttcgaggcc tggacgtgag actgacgcag
     1861 gtcaagaatc tgaaggacct gcacgcgttc aaggcgcacg tcgacgcgct cgcaatcgac
     1921 gtaccggtcg acgatgtcgt cgctccggac ggcgtcctcg gcgaaccggt gtcgttcacg
     1981 gacggcactg ccgggacgct gcagtcgccg aaccgctttg caatcctgcc gatggaaggc
     2041 tgggacgggt cgcctgaggg ccgaccgacc gatctcgtgc gccggcgttg ggaacggttc
     2101 gggcgcagcg gctgcggttt ggtgtgggcc gaggctacgg ccgtgcgtgc cgacggtcgc
     2161 gccaacccaa gccagttgct gctcgacgac acgacactcg acgactttgc ggcgttgcgt
     2221 gccctgctcg atccgagcca ggtcgcgggg ctccagctca cgcactcagg ccgctacgcg
     2281 tgcgcgcagc cacgcattgc ttaccggcac ccgatcctcg acgcgcgcgt cggtgctgac
     2341 gacgcgaatc tgctcagcga cgacgagctc gatgagctgg tcgcgtgcta tgtcgacgcg
     2401 gcggtccggg ctcgcacggc tggtttcgat ttcgtcgaca tcaagcattg ccacggatac
     2461 ctgctgcacg aactcctcag tgcacgcgac cgtccgggcc gtttcggtgg agacttcgaa
     2521 ggccgcacgc gatttctctc ctcggtcgta gaaggcatcc gttcgcgcgc gcccgatctc
     2581 gcaatcgcag tccggctttc ggcgttcgac ttcgtcccgt tcgttgccgg cgacgacaac
     2641 cgcggaacgc ccgagtggca cggtccatat ccgcacgcgt tcggcggcga cggcagcgga
     2701 accggcatcg acttacgcga gccgcacctg ctgctcgacc tgctggcgag catgggtgtg
     2761 aggcttgtga gcacgaccgc ggggagcccg tactacgtcc cgcacgtcca gcggcccgcg
     2821 tactttccgc cctcggacgg ctaccaaccg cccgaggatc cgctcgtcgg cgttgcccgg
     2881 caaattcacg cgacaagtga gatcaaacgt cgtcacccta atctcatcat cgtcggctcc
     2941 gcctacagtt acttgcagga atggctgccc tatgttgggc aagcgg
//
LOCUS       DASUGA010000675         4357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1527427, whole genome shotgun sequence.
ACCESSION   DASUGA010000675 DASUGA010000000
VERSION     DASUGA010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527427"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VFX21_13420"
     CDS             <1..616
                     /locus_tag="VFX21_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX5097018.1"
                     /translation="DFGKPVLCALARLGGHPVAVVANQPLALAGSIDADGAEKAAHFI
                     TVADSFHVPLVFLSDNPGVLPGSKSEQQGILRKGARMYAAQTLATTPKFEVTLRKAYG
                     FGSMVMSMIPFDGQSAVFAFPGATMGAMGAAAMSRARGSDEDEAALLRKREVEASYGS
                     AKTFGFDELIDPREIRNVLLHSLERALHRRQAPAEPVARIGITP"
     gene            625..1698
                     /locus_tag="VFX21_13425"
     CDS             625..1698
                     /locus_tag="VFX21_13425"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX5097019.1"
                     /translation="MTDREAVTTPWRRDASRIEAGLAAWAREWRGAATKLTDVRMPGS
                     GMANDTVLFRLDGDALVARLAPAADSFYPTFPTFDLDLQKRAMDLVRARTEVPAPEVV
                     HLEASTEWLDVPFMVVRAIEGEVASDNPPYLMDPSGWFLRGTPDDWQRLETSTIDVFV
                     QLHCIVDGVDTEFLRVDAPGETALARLLASHRDYYEWARDGHSVAVLERAFDVLTKTL
                     PSTERSVLLWGDGRPGNIIYRDFEPVGVLDWEMAGVGPPECDLAWATFFQRFWAAMAA
                     PYGVPVPAMFDRAASAATYERLSGTVLDDLAWYEALAGLRFGIILMRMGLRSIAYGQL
                     PMPDDIDELMMFPRLLEELLGEI"
     gene            complement(1695..2456)
                     /locus_tag="VFX21_13430"
     CDS             complement(1695..2456)
                     /locus_tag="VFX21_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX5097020.1"
                     /translation="MPAAKRTPTRTREKPQQIADDIRALIVSGKLSEGDSLGHEPDLV
                     ERFGVSRPSLREALRILEAEGLVTVVRGVRGGVVVHEPNGTMTARTAALVLQARNVAL
                     ADVFQARSIIEPIAARTVATMRGRRAAIAKLRALMEDEEAAIEEPEQFGVANAAFHNE
                     LVSRAGNQTLGIVAEMLTEIVVRAVTAVSRADDVAGSLATRRRGLRSQERLLELLEAG
                     DGPGAEEHWRSHMQAVGRVMLGQDASTVVDLLDHY"
     gene            complement(2470..3285)
                     /locus_tag="VFX21_13435"
     CDS             complement(2470..3285)
                     /locus_tag="VFX21_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5097021.1"
                     /translation="MTRAIDCLVNVDLGDQKPPDWMVRVKEDYFKGGDSFFKSPELSE
                     LIDEMDANGVERANLLCNVTATEGRALSFVEARPDRFALAVGGFNLLKPMPTVRALEV
                     FARNNPVVTALVGPSFWGDGMYPPTDPVYYPLYVKCCELDLALCMNMGIPGPPLPAEP
                     QNPIHLDRVCYRFPELKLCMIHGADPWWDTAIRLMLKYKNLRLMTSAWSPKRLPESLL
                     HYMRTRGKDRIIYASDYPVLSFERCLGEAAALDLPEDVRHAWLYDNADAFFGS"
     gene            complement(3282..>4357)
                     /locus_tag="VFX21_13440"
     CDS             complement(3282..>4357)
                     /locus_tag="VFX21_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HEX5097022.1"
                     /translation="ATPDALVGAMEEVVRPADDFVVVFTSGSRGVPKGTIHTHGSALG
                     AVASGLDARRVGADDRLYLPMPFFWTGGLAAGLLTALVAGATIITEAIPQPDRTLALL
                     EREHVTLFRGWPDQAATLAAHPGFATADLSSLRPGSLPAVLPPERRPAPGSRPNLFGM
                     TETFGPYCGAHGDVDLPAAKWGSCGRPLDGIEVRILEPDADGLGEIALRGRNVMRGIC
                     GRTREETFDADGFYASGDLGVLDADGYLWFHGRRDDMFKVNGATVYPSEVEAALRAIV
                     GVQQAHVTNVTVDHRETVGALVVSSLPLDELMRAVRARLSAFKAPTCWVVTDSGDDVP
                     LTATAKVDKPALQELLTRKGTGR"
BASE COUNT          677 a         1489 c         1493 g          698 t
ORIGIN      
        1 tgacttcggc aaaccggtgc tctgcgcgct ggcgcgactc ggcgggcatc ccgtagccgt
       61 tgtcgcgaac cagccgctgg cgctggcggg gtcgatcgac gccgacggcg cggagaaggc
      121 ggcccacttc atcaccgtgg ccgactcgtt ccacgtgccg ctcgtgttct tgtcggacaa
      181 tcccggcgtg ctgcccggca gcaagtcgga acagcaaggc atcctgcgca agggcgcccg
      241 catgtacgcg gcacaaacgc tggcgaccac accgaagttc gaagtgacgc tgcgcaaggc
      301 gtacggcttc gggtcgatgg tcatgagcat gattccgttc gacggccagt cggcagtgtt
      361 cgcgttcccc ggcgccacga tgggcgcgat gggcgcggcc gcgatgagcc gggcgcgcgg
      421 gtcggatgaa gacgaggccg cgttgttgcg aaagcgcgaa gtggaggcct cgtacgggtc
      481 ggcgaagacg ttcggcttcg acgagctcat cgatccgcgg gagatccgca acgtgctgct
      541 gcactccctc gagcgcgcac tacaccgccg ccaagcaccg gccgagcccg tcgcccggat
      601 cggcatcacg ccgtaagtgc tccggtgacc gatcgcgaag ccgtcaccac gccgtggcgg
      661 cgtgacgcca gtcgcatcga agccggcctc gcggcgtggg cgcgcgaatg gcggggcgcg
      721 gccacgaagc tcaccgacgt acgcatgccc ggcagcggca tggccaacga cacagtgttg
      781 ttccggctcg acggcgacgc gctcgtcgct cgcctcgcac cggcggccga ctcgttctat
      841 ccgacgttcc cgacgttcga cctcgacctg cagaagcggg cgatggacct ggtccgcgcc
      901 cgcaccgagg tgccggcgcc cgaagtcgtg cacctcgaag cctcgacgga atggctcgac
      961 gttcccttca tggtcgtgcg ggcgatcgag ggcgaggtcg cgtccgacaa cccgccgtat
     1021 ctgatggacc cgagcggttg gttcctgcgg ggtacccccg acgattggca gcgactcgag
     1081 acgtcgacga tcgacgtgtt cgtgcagctg cactgcattg tcgacggagt cgacaccgag
     1141 ttcctgcgcg tcgacgcgcc cggcgagacc gcgctggcgc gattgttggc ctcgcaccgc
     1201 gactactacg agtgggctcg tgacggtcac tcggtcgccg tgctggagcg cgcgttcgac
     1261 gtcctgacga agacattgcc gtcgacggag cgcagcgtgc tgctctgggg cgacggccgg
     1321 cccggcaaca tcatctaccg cgacttcgaa cccgtcggcg tgctcgactg ggagatggcg
     1381 ggcgtcggtc cacccgagtg cgacctcgcg tgggcgacct tcttccagcg gttctgggcc
     1441 gccatggccg cgccgtacgg cgtgccggtc ccggccatgt tcgaccgcgc ggcgtcagcc
     1501 gcgacgtacg agcgattgtc gggcaccgtg ctcgacgacc tcgcatggta cgaagcgctc
     1561 gccggactgc gcttcggcat catcctcatg cgcatgggac tgcgcagcat tgcgtacgga
     1621 cagctgccga tgcccgacga catcgacgag ttgatgatgt tcccgcgcct gctcgaagag
     1681 ttgttgggag agatctagta gtggtcgagg aggtcgacga ccgtcgatgc gtcctggccg
     1741 agcatcacgc gcccgacggc ctgcatgtgc gagcgccagt gctcttcggc tcccggaccg
     1801 tcgcccgctt cgagcagctc gagcagccgc tcctgtgaac gcagaccgcg gcgccgggtc
     1861 gcgagcgatc ccgcgacgtc gtcggcgcgg ctgaccgcgg tcactgcgcg caccacgatc
     1921 tcggtcagca tctcggcgac gatgccgagc gtctggttgc cggcccgcga gaccagctcg
     1981 ttgtggaacg cggcgttggc gacgccgaac tgctcgggct cctcgatggc ggcctcttcg
     2041 tcctccatca acgcacggag cttggcgatc gcggcccgcc gtccgcgcat cgtcgcgacg
     2101 gtgcgcgccg cgatcggctc gatgatgctg cgggcctgga acacgtcggc cagcgcgacg
     2161 tttcgcgcct gcaacacgag cgccgccgtg cgtgcggtca tcgtcccgtt gggttcgtgc
     2221 acaacgacgc cgccgcgcac gccgcgcacg acggtcacca aaccttcggc ttcgaggatg
     2281 cgcaacgctt cccgcaacga cggtcgcgac acgccgaacc gctccacgag atcgggttcg
     2341 tgaccgagcg agtcaccttc ggagagcttg ccggacacga tcaacgcgcg gatgtcgtcc
     2401 gcgatctgct gcggcttctc ccgggttctc gtcggcgtgc gcttggccgc gggcatcgaa
     2461 cggttccttt cagcttccga agaaggcgtc ggcgttgtcg tacagccagg cgtgccgtac
     2521 gtcttcggga aggtcgagcg ccgccgcttc accgagacaa cgttcgaagg agagcaccgg
     2581 atagtccgac gcgtagatga tgcggtcctt gccgcgcgtg cgcatgtagt gcaggagcga
     2641 ctcgggcaat cgcttcggtg accacgcgga cgtcatcagc cgcaggttct tgtacttcaa
     2701 catcagccgg atcgcggtgt cccaccacgg gtcggcgccg tggatcatgc acagcttgag
     2761 ctcgggaaag cggtaacaga cgcggtcgag gtggatcggg ttctgcggtt cggcgggcag
     2821 cggcggcccg gggatgccca tgttcataca cagcgcgagg tcgagctcgc agcacttcac
     2881 gtacagcggg tagtagacgg gatcggtcgg cgggtacatg ccgtcacccc agaagctcgg
     2941 tcccaccagt gccgtcacga ccgggttgtt gcgcgcgaac acctcgagcg cgcgcacggt
     3001 cggcatcggc ttcagcaggt tgaacccgcc gaccgcgagc gcaaaacgat cgggccgcgc
     3061 ctcgacgaac gagagcgcgc gaccctcggt cgcggtgaca ttgcacaaca agttcgcgcg
     3121 ctccacgccg ttggcgtcca tctcgtcgat cagctccgag agctcagggc tcttgaagaa
     3181 cgagtcgccg cctttgaagt agtcctcctt gacgcgcacc atccagtcgg gcggcttctg
     3241 gtcaccgagg tcgacgttca cgaggcagtc aatggcacgg gtcatcgccc ggtccccttt
     3301 cgtgtcagca gctcctgcag cgcgggcttg tcgaccttcg cggtcgcggt caggggcacg
     3361 tcgtcgcccg agtcggtgac gacccagcac gtcggcgctt tgaacgcact caggcgcgcg
     3421 cgcactgctc gcatcagctc gtcgagcggc agcgacgaca cgacgagcgc accgaccgtc
     3481 tcgcgatggt cgacggtgac gttggtgacg tgcgcctgct ggacgccgac gatcgcgcgc
     3541 aatgcggctt cgacctccga cggatagacg gtggcgccgt tgaccttgaa catgtcgtca
     3601 cggcgaccgt ggaaccacag gtagccgtcg gcatcgagca cgccgaggtc gccggatgcg
     3661 tagaagccgt cggcatcgaa cgtctcttcg cgagttcgcc cgcagatccc ccgcatcacg
     3721 ttgcgcccgc ggagcgcgat ctcgccgagg ccgtcggcgt cgggttcgag gatgcgcact
     3781 tcgatgccgt cgagcgggcg gccgcagctg ccccacttcg ccgcgggcag gtcgacgtct
     3841 ccgtgcgcac cgcagtacgg tccgaacgtc tcggtcatgc cgaagaggtt cggacgcgag
     3901 ccgggtgccg gccgccgctc gggtggcagg accgcgggaa ggctgcccgg ccggagactc
     3961 gacagatccg ccgtcgcgaa ccccgggtgc gcggcgagcg tcgccgcttg gtcgggccac
     4021 ccgcggaaca acgtcacgtg ttcgcgttcg aggagcgcga gtgtgcgatc gggttggggg
     4081 atcgcctcgg tgatgatcgt cgcgcccgcg acgagcgcgg tcaggaggcc ggccgcgagc
     4141 ccaccggtcc agaagaacgg catcgggagg tacaaccggt cgtcggcgcc gacgcgccgc
     4201 gcgtcgagcc cggacgcgac tgcccccagc gcgctgccgt gcgtatggat cgttcccttc
     4261 ggcacacccc gactcccgga ggtgaacacg acgacgaaat cgtccgccgg ccgcaccacc
     4321 tcctccatcg cgcccaccaa cgcatcgggc gtcgctg
//
LOCUS       DASUGA010000676         9186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1436936, whole genome shotgun sequence.
ACCESSION   DASUGA010000676 DASUGA010000000
VERSION     DASUGA010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 9186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9186
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436936"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFX21_13445"
     CDS             <1..265
                     /locus_tag="VFX21_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097023.1"
                     /translation="MVMLFDEPTSALDPEMIREVLDVMRDLAEQGMTMVVVTHEMGFA
                     SAAATKVVFMDEGRIVESGAPDQVLRHPTHERTQMFFDKVLHH"
     gene            262..777
                     /locus_tag="VFX21_13450"
     CDS             262..777
                     /locus_tag="VFX21_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019980392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5097024.1"
                     /translation="MIEVRVARPDEYKRVGDLTVDSYRALSVDHLWGGYDTDILDVAG
                     RAEHAEILVAVDGDEIVGAVTFVSEPGPWLEWTQPGEVQFRLLAVDRAVQGRGIGETL
                     ARACIERAAGRPICIHTTEWMPAARRLYERLGFVRRPDRDVHYEEWHEKGFDDLPDIW
                     VGKPFMAYRYE"
     gene            complement(768..2144)
                     /locus_tag="VFX21_13455"
     CDS             complement(768..2144)
                     /locus_tag="VFX21_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5097025.1"
                     /translation="MSLVDLLDGDDADVVAIAGAASSTRGEVRALADTLADQLQAYDV
                     AGRAVGVRMPNTAAAIAAWFAVWRSGAAFVPLNPRVPDAEIERLVETTGVVVIVEAGS
                     PYTVRPVTPIQARRIEEQQAIIQFTSGTTGLPKAVPLRHDTVGELLDGVIGELRSGRA
                     DRPKMPNIVPVSLSLWAGIYQVLFAWKLGVPVVLMERFAPREFARLVKQHEIRSSVLP
                     PAALVMLMQDDTITTLKPLRYVRSVSAPLSPAAARRFHERFGIAVLNGYGQTELGGEA
                     VGWTAADWKQFGNEKLGSVGRPHAAFSLRVNDDDELEIKSSHALPPADAAMEGRVTAD
                     GWLRTGDLARIDDDGFVWIEGRVSDMINRGGLKVFPDDVEEQLRAFPGVLDVGVAGVA
                     DERLGEVPWAFVVPRANAYIDAGELRRWAHDRMAPYKVPVGVTVVDDLPRNEIGKLMR
                     AELVRRYS"
     gene            complement(2141..2818)
                     /locus_tag="VFX21_13460"
     CDS             complement(2141..2818)
                     /locus_tag="VFX21_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097026.1"
                     /translation="MTEYVTVGTAVIAYIEPHEGRARAFNAWYERDHFYAAAMAGPGC
                     YAGARWVATRDCKESRTEGWLFGDNAVGSYLATYWILPGKQEEWDEWIAREYGSMPPE
                     RLFAARDHLHTAVYDFAWDSRADGAPIPATALDHGFAGVVVTGWTDGGAAREWAEWII
                     DAEVPLVMAFSPQRTIMSAANPPPHKLVLAFTRMDPRLTPLPLDAEYGSAFLATIPGT
                     DTYVDEL"
     gene            complement(2815..3633)
                     /locus_tag="VFX21_13465"
     CDS             complement(2815..3633)
                     /locus_tag="VFX21_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5097027.1"
                     /translation="MNYPQLETLLLERRGPVGWLINNRPEQLNAMSARMRDEFEVAWK
                     TLDEDPEVRVIVHTGEGRAFQTGVDVTELATDGQGMERYRESVENWTLRFTSMQNDVW
                     KPVITAVNGICAGGAFHWVADADIVIAASDAQFFDPHVSVGQVVSIEAIGLMRKMPVE
                     AVMRMAFVGRHERMSAARAYELGMISQVVDPPERLRDEAQALAEKIAQNSPAAMAATK
                     RALWGALELGLTDACRAGAAELTSMWGHPDQEEGPAAFAAKRPANWQPLTPGAS"
     gene            complement(3630..4316)
                     /locus_tag="VFX21_13470"
     CDS             complement(3630..4316)
                     /locus_tag="VFX21_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5097028.1"
                     /translation="MGTYETLVVERRGPVGWLVFKRPDAGNAMDARMMAELEDAWRAL
                     DADPDVHVIVNTGEGRAFQTGLDVVQLSKDRNALREQSRRTRDFALRFTAWHNEVWKP
                     VIAAVNGTVAGGGLHFVADADIVVMAANASFVDPHVSIGQVSAFETIALAKKSPKEAV
                     LRMALIGTGERVDARRAYQLGFCSQVVDPPERLRDEAQALAEKIASNSPAALAATKRA
                     LWGALERKGA"
     gene            complement(4321..5556)
                     /locus_tag="VFX21_13475"
     CDS             complement(4321..5556)
                     /locus_tag="VFX21_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009956481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5097029.1"
                     /translation="MTLAVGPETADDAVLAAVRAWVDEHVPRSWIEAGDAGGAAAIRA
                     VRPRAAYEEWYPVFAASGLVVPTWPVPYGGLDLSPRVARLVEQVLAPYNLGRLNPLGL
                     NNAAPALFAYGTEEQRLRYLPPIVRNEEKWCQLFSEPGAGSDLASLATRAVRDGDVWI
                     VSGQKVWTTWAHISDYGVLLARTDADVPKRQGITYFLVDLHQPGVDVRPLRHIGGEVD
                     FNEVWLDGAVVPDDMRIGPVGEGWKVAGATLSGERQMVAGAGSGGVDRIGGRGTDALL
                     ELARARGRDGDPCVRDALMRVYSEERIRAWTNQRVRAQLKSGRPPGPESSVGKVHQGT
                     LNQRIQMLAVDVLGADALAWSGDGLPPEVRGMLRSRANTIEGGTTEVNKNILGERVLG
                     LPREPDPYHGASWRDIPRS"
     gene            complement(5553..6326)
                     /locus_tag="VFX21_13480"
     CDS             complement(5553..6326)
                     /locus_tag="VFX21_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018637204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5097030.1"
                     /translation="MLDGSALRLQLNRSDKRNAISEPMFLALVDAVTRASTDERVRVI
                     VLEGAGDHFCGGADIVARNAGDGPRPSAGSIQRRVPTQAHRLIPLLRTVQVPVVARVQ
                     GWAAGIGFQLALAADFCIAADDAKFWEPFTERGFTPDSGATWLLPRRVGMTRARELLL
                     LGRALSGAEAAEWGAIHGAVPAGELDAAVDEVVTQLASGPTVALGLSKWLLSQGEERA
                     LTDQLADEAFALELSSRSEDFREGLAAFKERRPPNFEGR"
     gene            complement(6365..7615)
                     /locus_tag="VFX21_13485"
     CDS             complement(6365..7615)
                     /locus_tag="VFX21_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097031.1"
                     /translation="MAETGQITDEGVARLRARIGIPEPHPLPPYYFLPTYDTFRNVAY
                     AYGDDNPLWCDPSYGAASRWGSVIASPVLVGGDTLIGEDEVTEVAAEHRDLMKGDPLR
                     GVHAFYSASAREWWAPLYPLRRTFRRNALVAALDKQGEFAGRAIHEWTAQVFRDETGT
                     LLSGQYRLMVRTEREKARVRKKNDAVVIEPYTDEQIDEIEAQYERERPRGAEPRWWED
                     VQEGDEVGPMVKGPMTVTDMICWHAGMGMGLYGVKPLRLGAQNRRRIPRFFHRDELNV
                     PDVMQRVHWDPEFARKAGNPTIYDYGRMRETWLIHLCTDWMGDDAWLWKLDCEFRKFN
                     YVGDTQWLRGRVTRKFLAAGDRPAVELELEARNQRDEVTTPGHATVLLPSREHGPVRL
                     PGPPVGNGDLQDALLAISAEFSRT"
     gene            complement(7632..9089)
                     /locus_tag="VFX21_13490"
     CDS             complement(7632..9089)
                     /locus_tag="VFX21_13490"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5097032.1"
                     /translation="MKHGRWLAIVITVALGAAACGRDSETTDSGTATTAAGTETTAGG
                     DGGNESAGLDQGAFGDLGVLCAPAPDDEPNAAGTDPGVTADKIQISTFSDPGFSGRLG
                     LNQEMFDTATAFTNWCNEKGGIHGRQIDLKLRDAKLTEFQQRVIEACDEGDFMTVGGG
                     AVFDDTGQPDRLACALPAIPGYAVTAAASDADLSLQPVPLPGDEQPIGHLRYLAEKYP
                     DTIENVGIFTGSLETTIAVANRNKEAMEANGFKIVYEGQYNPQGETSWRTFIEAQRSA
                     GVKGLYWVGEPANLAAFLTEASSVGMTYDWVTADANHYDPGLLDAGAAANKTYVRSVF
                     YPFLDEAQAKENPATEQYRELINQYTDNGKIAYLGVQGLSSWLLFAKAAGECGAELTR
                     DCVWEKATAITDWTGGGLHAKQNLETQGASECVSIVEVVDGKFQLVDDLEPNDGIYLC
                     DPKNVAKLTGDYGTGAKCPNPAYASDPKPSNCANG"
BASE COUNT         1330 a         3280 c         3112 g         1464 t
ORIGIN      
        1 gatggtgatg ctgttcgacg agccgaccag cgcgctcgac cccgagatga tccgcgaagt
       61 cctcgacgtc atgcgcgact tggccgagca gggcatgacg atggtcgtcg tgacgcacga
      121 gatgggcttc gcgagcgcgg ccgcgacgaa ggtcgtgttc atggacgaag gccgtatcgt
      181 cgagtcgggg gcgcccgacc aggtgttgcg tcatcccacg cacgagcgga cgcagatgtt
      241 cttcgacaag gtgctgcacc attgatcgag gttcgagttg cgcgtcccga cgagtacaag
      301 cgagtcggtg acctgaccgt cgactcgtac cgcgcgctct cggtcgacca tctctggggc
      361 ggctacgaca ccgacatcct cgatgtcgcg ggccgcgccg agcacgcaga gatcctcgtc
      421 gcggtcgacg gtgacgagat cgtcggcgcg gtgacgttcg tgtccgaacc cggtccgtgg
      481 ctcgagtgga cgcaaccggg cgaggtgcag ttccggttgc tcgcggtcga ccgcgccgtg
      541 caggggcgcg ggatcggcga gacgctcgcg cgcgcgtgca tcgaacgggc cgcgggccgg
      601 ccgatctgca tccacaccac cgagtggatg cccgccgctc gccgtctcta cgaacgactc
      661 gggttcgtcc gccggcccga ccgcgacgtg cactacgagg agtggcacga gaaaggcttc
      721 gacgacttgc ccgacatctg ggtcggcaag ccgttcatgg cgtaccgcta cgagtagcgg
      781 cggacgagct cggcgcgcat cagcttgccg atctcgttgc gcgggaggtc gtcgacgacg
      841 gtgacgccga ccgggacctt gtagggcgcc atgcggtcgt gggcccagcg ccggagctcg
      901 ccggcgtcga tgtacgcgtt cgcacgcggc acgacgaacg cccacggcac ttcgccgagc
      961 cgctcgtcgg cgacacccgc gacgccgacg tcgagcacac ccgggaacgc gcgcagctgc
     1021 tcctcgacgt cgtcggggaa caccttcaaa ccgccgcggt tgatcatgtc gctcacgcgc
     1081 ccctcgatcc atacgaagcc gtcgtcgtcg atgcgggcga ggtcaccggt gcgtaaccac
     1141 ccgtcggcgg tgactcgtcc ctccatcgcc gcgtcggcgg gcggcagcgc gtgcgacgac
     1201 ttgatctcca gctcgtcgtc gtcgttcacc cgcaacgaga aggcggcatg gggccgtccg
     1261 accgagccga gcttctcgtt gccgaactgc ttccagtcgg ccgcggtcca cccgaccgct
     1321 tcgccaccga gctcggtctg cccgtagccg ttcaacacgg cgatgccgaa gcgttcgtgg
     1381 aagcgccggg cggcagccgg tgacaacggc gcggacacgc tgcgcacgta gcgcaacggc
     1441 ttcagtgtcg tgatcgtgtc gtcctgcatc aacatgacga gcgccgcggg cggcaacacc
     1501 gacgagcgga tctcgtgttg cttcacgagt cgcgcgaact cgcgcggagc gaacctctcc
     1561 atgagcacga ccggcacgcc gagcttccac gcgaacagca cctggtagat gccggcccac
     1621 aacgacagcg agacgggaac gatgttcggc atcttcggcc ggtcggcacg gccgctgcgc
     1681 aactcgccga tcacgccgtc gagcagttcg cccacggtgt cgtgccgcaa cgggaccgcc
     1741 ttgggcagcc ccgtcgtacc cgacgtgaac tggatgatcg cctgctgctc ctcgatacga
     1801 cgggcttgga tcggcgtgac gggccgcacc gtgtagggcg agccggcctc gacgatgacg
     1861 accacgcccg tcgtctcgac cagccgctcg atctccgcgt cgggcacacg ggggttcagc
     1921 ggcacgaacg cggcgccgct gcgccacacc gcgaaccagg ccgcgatcgc ggcggcggtg
     1981 ttcggcatgc ggacaccgac cgcgcggccc gcgacgtcgt aggcctgcaa ctgatcggcc
     2041 agcgtgtcgg ccagtgcgcg cacctcgcca cgcgtcgacg aggccgcgcc ggcaatggcg
     2101 acgacgtcgg catcgtcgcc gtcgaggagg tcgacgaggc tcacagctcg tcgacgtagg
     2161 tgtcggtgcc gggaatcgtc gcgagaaagg cgctgccgta ctcggcgtcg agcgggagcg
     2221 gcgtcaaacg gggatccatg cgcgtgaacg cgagcacgag cttgtgcggc ggcggattgg
     2281 cggccgacat gatcgtgcgc tggggcgaga acgccatcac cagcggcact tcggcatcga
     2341 tgatccactc cgcccactca cgcgcggcgc caccgtccgt ccatcccgtg accacgacgc
     2401 cggcgaatcc atggtcgagc gcggtcgccg gaatcggcgc accgtcggcg cgggagtccc
     2461 acgcgaagtc gtagaccgcg gtgtggagat gatcgcgtgc ggcgaacaat cgctccggcg
     2521 gcatcgaccc gtattcacgc gcgatccact cgtcccactc ctcctgcttg ccgggcagga
     2581 tccagtacgt cgcgagatac gagccgacgg cgttgtcacc gaatagccag ccctcagtgc
     2641 gcgactcctt gcaatcgcgc gtcgcgaccc agcgggcacc cgcgtagcag cccgggccgg
     2701 ccatcgccgc cgcgtagaaa tgatcacgct cgtaccacgc gttgaacgcg cgggccctgc
     2761 cttcgtgcgg ttcgatgtac gcgatgaccg cggtgccgac ggtgacgtac tcggtcatga
     2821 cgcgccgggc gtgagtggct gccagttcgc gggccgcttc gccgcgaacg cggccggccc
     2881 ttcctcctgg tcggggtgac cccacatcga cgtcagctcg gccgcgccgg cccggcacgc
     2941 gtcggtgagc ccgagctcga gcgcgcccca caacgcgcgc ttcgtcgcgg ccattgccgc
     3001 gggcgagttc tgcgcgatct tctcggccag cgcctgcgct tcgtcgcgca acctctcggg
     3061 cgggtcgacg acctggctga tcatgcccag ctcgtacgcg cgcgccgcgc tcatccgctc
     3121 gtggcggccg acgaacgcca tgcgcatcac cgcttcgacg ggcatcttgc gcatgagccc
     3181 gatcgcttcg atggagacga cctgacccac cgacacgtgc gggtcgaaga actgcgcgtc
     3241 gctcgccgcg atgacgatgt cggcgtcggc gacccagtgg aacgcgccgc ctgcgcagat
     3301 gccgttgacc gcggtgatca ccggcttcca cacgtcgttc tgcatcgacg tgaaccgcag
     3361 cgtccagttc tcgaccgact cgcggtagcg ctccatgccc tggccgtcgg tcgcgagctc
     3421 ggtcacgtcg acacccgttt ggaacgcacg gccctccccc gtgtgcacga tgacgcgcac
     3481 ctcgggatcc tcgtcgagcg tcttccacgc aacctcgaac tcgtcgcgca tgcgcgcgct
     3541 catcgcgttc agttgctcgg gacggttgtt gatcaaccag cccacgggtc cgcgccgttc
     3601 gagcaacagc gtctcgagct gcgggtagtt cacgcgccct tcctctccag cgcgccccac
     3661 agcgcgcgct tggtcgcggc caacgcggcc ggcgagttcg atgcgatctt ctcggcgagc
     3721 gcctgtgctt cgtcgcgcag ccgctcgggc gggtcgacga cctggctgca gaagcccagt
     3781 tggtacgcgc gccgggcgtc gacgcgctct cccgtcccta tcagcgccat gcggaggacg
     3841 gcttccttgg gtgacttctt ggccaaggcg atcgtctcga acgcgctgac ctgaccgatc
     3901 gagacgtgcg ggtcgacgaa cgacgcgttc gcggccatca cgacgatgtc ggcgtcggcg
     3961 acgaagtgca ggccgccgcc cgcgacggtg ccgttcacgg ccgcgatcac cggcttccag
     4021 acctcgttgt gccaggccgt gaaccgcagt gcgaagtcgc gggtgcggcg cgactgttcg
     4081 cgcagtgcat tacggtcctt gctgagttgc acgacgtcga gccccgtctg gaacgcgcgc
     4141 ccttcgcccg tgttcacgat gacgtggaca tccggatcgg cgtcgagcgc gcgccacgcg
     4201 tcctcgagct cggccatcat gcgcgcgtcc atcgcgttcc cggcgtcggg ccgcttgaac
     4261 acgagccagc ccaccggccc gcgccgttcg acgaccaacg tctcgtacgt gcccaccgcg
     4321 tcagctccgc gggatgtcgc gccacgacgc gccgtggtac ggatccggct cgcgcggcag
     4381 acccagaact cgttcgccga ggatgttctt gttcacctcg gtcgtgccgc cttcgatcgt
     4441 gttggcgcgg ctgcgcagca tgccgcgcac ctcgggcggc aacccgtcgc cggaccacgc
     4501 cagggcgtcg gcgccgagca cgtcgaccgc gagcatctgg atgcgctggt tgagcgtccc
     4561 ctggtggacc ttgccgaccg agctctccgg gcccggcggt cgacccgact tgagctgggc
     4621 ccgcacccgc tggttcgtcc acgcccggat gcgttcttcg gagtacacac gcatgagcgc
     4681 gtcgcgcaca cacggatcgc cgtcgcgacc gcgcgcccgc gccagctcca gcagcgcgtc
     4741 ggtgccgcgg ccgccgatgc ggtcgacgcc gccggaaccc gcgcccgcga ccatctgccg
     4801 ttcgccggac agcgtcgcgc cggcgacctt ccagccctcg ccgacgggac cgatgcgcat
     4861 gtcgtcgggg acgaccgcac cgtcgagcca cacttcgttg aagtcgacct cgccgccgat
     4921 atggcggagc gggcgcacgt cgacgcccgg ctggtggagg tcgacgagga agtaggtgat
     4981 gccctgccgc ttcggcacgt cggcatcggt gcgggcgagc aacacgccat agtcggagat
     5041 gtgggcccag gtggtccaca ccttctgacc gctcacgatc cacacgtcgc cgtcgcgcac
     5101 cgcgcgcgtc gcgagtgacg cgaggtcgga tccggcaccc ggttcgctga agagttggca
     5161 ccacttctct tcgttgcgca cgatcggcgg caggtatcgc agccgctgct cctcggtgcc
     5221 gtacgcgaac aacgccggcg ccgcgttgtt cagcccgagc ggattgaggc gacccaggtt
     5281 gtacggcgcg agcacctgct cgaccaggcg cgccacgcgc ggcgacaggt cgagcccgcc
     5341 gtagggaacc ggccacgtcg ggaccacgag ccccgacgcg gcgaagaccg ggtaccactc
     5401 ctcgtacgcg gcgcgcggcc gcaccgcgcg gatggcggcc gcgcccccgg catcgccggc
     5461 ttcgatccac gagcgcggga cgtgctcgtc gacccatgcc cgcaccgcgg ccaggaccgc
     5521 gtcgtcggcg gtctccggac cgacggcgag cgtcatcgcc cctcgaagtt cggcgggcgc
     5581 cgctccttga acgcggccag accttcgcgg aaatcctcgc tgcgcgacga cagctcgagc
     5641 gcgaaggctt cgtcggcgag ctggtcggtc agcgcgcgct cctcgccttg cgacagcaac
     5701 cacttcgaca ggccgagcgc aaccgtcggc cccgacgcga gctgcgtgac gacctcgtcg
     5761 accgcggcgt cgagctcgcc ggccggtacc gcgccgtgga tcgcgcccca ctcggccgcc
     5821 tcggctccgc tcagggcgcg cccgagcagc agcagctcgc gcgcgcgggt catgccgacc
     5881 cggcgcggca gcaaccacgt ggcgccgctg tcgggggtaa agccgcgttc ggtgaacggc
     5941 tcccagaact tcgcgtcgtc ggccgcaatg cagaagtcgg cggccagcgc gagctggaaa
     6001 ccgatgcccg cggcccagcc ctgcacgcgg gcgacgaccg gcacctgcac ggtgcgcagc
     6061 aacgggatca ggcggtgggc ctgcgtcggc acccgccgct ggatgctgcc cgcgctcggc
     6121 cgcgggccgt caccggcgtt gcgggcgacg atgtcggcgc cgccgcagaa gtgatcgccg
     6181 gcaccctcga gcacgatgac gcgtacgcgc tcgtcggtgc tcgcgcgggt gaccgcatcg
     6241 acgagcgcga ggaacatcgg ctccgagatc gcgttgcgct tgtcggagcg gttcagctgc
     6301 aggcgaagtg ccgagccgtc gagcatgacg tcgagcccgt cgaccgatgg gtaggccacg
     6361 gccgtcatgt tcgcgagaac tcggcggaga tcgccaacag cgcgtcttgg agatcgccgt
     6421 tgccgaccgg aggaccgggc agacgcaccg gcccgtgctc gcgactcggc aacagcaccg
     6481 ttgcgtggcc gggtgtcgtc acctcgtcgc gctggttgcg cgcttcgagc tcgagctcga
     6541 ccgccggccg gtcgccggcc gcgaggaact tgcgcgtcac ccggccgcgg agccactgcg
     6601 tgtcaccgac gtagttgaac ttgcggaact cgcaatcgag cttccacaac cacgcgtcgt
     6661 cgcccatcca gtcggtgcag aggtggatga gccacgtctc gcgcatgcgg ccgtagtcgt
     6721 agatggtcgg gttgccggcc ttgcgcgcga actccgggtc ccagtgcacg cgttgcatga
     6781 cgtcgggcac gttcagctcg tcgcgatgga agaagcgcgg gatgcggcgg cggttctgcg
     6841 cgcccaaccg cagcggcttc acgccgtaca ggcccatccc catgcctgcg tgccagcaga
     6901 tcatgtcggt gaccgtcatc ggccccttca ccatcgggcc gacctcgtcg ccctcctgca
     6961 cgtcttccca ccagcgcggc tccgcgcccc gcggccgctc gcgctcgtac tgcgcctcga
     7021 tctcgtcgat ctgttcgtcg gtgtacggct cgatcaccac cgcgtcgttc ttcttgcgca
     7081 cccgcgcctt ctcgcgttcg gtgcgcacca tcagccggta ttggcccgag agcaacgtgc
     7141 ccgtctcgtc gcggaacacc tgcgccgtcc actcgtggat cgcgcgaccc gcgaactcgc
     7201 cctgcttgtc gagcgcggcg acgagcgcat tacggcggaa cgtccggcgc aacggataca
     7261 acggcgccca ccactcccgc gcgctcgcgg agtagaaggc gtgcacgccg cgcagcgggt
     7321 cgcccttcat gagatcgcga tgctcggccg cgacttcggt gacttcgtcc tcgccgatga
     7381 gcgtgtcgcc gcctacgagc accggcgacg cgatcacgct cccccaacgc gacgccgcgc
     7441 cgtacgaagg gtcgcaccac agcgggttgt cgtcgccgta cgcgtacgcg acgttgcgga
     7501 acgtgtcgta cgtcggcagg aagtagtagg gcggcagcgg atgcggttcg gggatcccga
     7561 tgcgggcgcg cagtcgtgcg acgccctcat cggtgatctg gcccgtctcc gccacgcgcc
     7621 ctcgtctctc cctatccgtt cgcgcagttc gacggcttgg ggtcgctggc gtacgccggg
     7681 ttggggcact tggcgcccgt gccgtagtcg cctgtgagct tcgcgacgtt cttcgggtcg
     7741 cacaggtaga tgccgtcgtt cggttcgagg tcgtcgacga gttggaactt gccgtcgacg
     7801 acttcgacga tcgacacgca ctccgacgcg ccctgcgtct ccaggttctg cttcgcgtgc
     7861 aacccaccgc cggtccagtc ggtgatcgcg gtcgccttct cccacacgca gtcgcgcgtc
     7921 agttcggccc cgcactcacc cgccgccttg gcgaacagca gccagctcga caggccctgc
     7981 acgccgaggt acgcgatctt gccgttgtcg gtgtactggt tgatgagctc gcggtactgc
     8041 tcggtcgccg ggttctcctt ggcctgtgcc tcgtcgagga acgggtagaa gaccgaacgc
     8101 acgtaggtct tgttcgccgc ggcgccggcg tcgagcagac ccgggtcgta gtggttggca
     8161 tcggcggtca cccagtcgta ggtcatgccg acactcgacg cttcggtgag gaacgccgcg
     8221 aggttggcgg gctcgcccac ccagtacagg cccttcacgc ccgcgctgcg ttgcgcctcg
     8281 atgaacgtgc gccagctcgt ctcgccctgc gggttgtact ggccttcgta cacgatcttg
     8341 aagccgttgg cttccatcgc ttccttgttg cggttcgcaa ccgcgatcgt cgtctcgagc
     8401 gaaccggtga agatgcccac gttctcgatc gtgtcggggt acttctccgc gaggtagcgg
     8461 aggtggccga tgggctgctc gtcaccgggg agcggcacgg gctgcagcga caggtccgcg
     8521 tcggacgccg ccgcggtcac cgcgtaaccg gggatcgcgg gcagggcgca cgcgaggcgg
     8581 tcgggttggc cggtgtcgtc gaacaccgcg ccaccgccga cggtcatgaa gtcaccctcg
     8641 tcgcatgctt cgatgacgcg ctgctggaac tcggtgagct tcgcgtcgcg cagcttgagg
     8701 tcgatctgac gaccgtggat gccgcccttc tcgttgcacc agttggtgaa cgcggtggcg
     8761 gtgtcgaaca tctcctggtt cagaccgagc cgaccggaga agccggggtc ggagaaggtc
     8821 gagatctgga tcttgtcggc cgtgacaccg gggtcggtgc cggccgcgtt cggctcgtcg
     8881 tcgggggccg gcgcgcagag cacgccgagg tcgccgaacg cgccttggtc gagcccggcg
     8941 gattcgttcc cgccgtcgcc gcccgcggtg gtctcggtac cggcggccgt cgtcgcggtt
     9001 ccggagtcgg tggtttcact gtcacgcccg cacgccgccg cgccgagtgc gaccgtgatg
     9061 acgatcgcga gccaacgccc gtgtttcatc gtgtgctctc cagggtcggg ggcacgtacc
     9121 ttatgcaacg tgcctgacga gaacctgccg cccaccctgc ccggaatcgt ggcccgcgcg
     9181 gcccac
//
LOCUS       DASUGA010000677         3844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1490980, whole genome shotgun sequence.
ACCESSION   DASUGA010000677 DASUGA010000000
VERSION     DASUGA010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490980"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VFX21_13495"
     CDS             <1..734
                     /locus_tag="VFX21_13495"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5097033.1"
                     /translation="GVPLEDAAQFHQWAEQINTGPLQPEAGHAASQAMVDYLRPLVEE
                     RRANPGDDFLSDLVHAEVDGEQLTDGKLYGFLRLLLPAGAETTYRVMGNCLYALLTHP
                     DEMARVLDDRALIPDVIEETLRWETSVTMVSRIATKDAEVSGCPIKAGSPVGVLTGSA
                     NHDEDRWDDASEWKLGRATQHHIAFGTGPHQCLGMHLARLELRVGLDVILDRLPNLRL
                     DDGAEGAFIEGYAFRGPRALPVTFG"
     gene            complement(761..1483)
                     /locus_tag="VFX21_13500"
     CDS             complement(761..1483)
                     /locus_tag="VFX21_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5097034.1"
                     /translation="MPSTTDRFSFRQLLAGRDFARDDQIAQQMVNFVYVIGDRETGSA
                     ALIDPAYGVRELVELLDADGLRCTDVLVTHYHPDHVGGSLGGWSIEGLAELREMGVDA
                     RIHVQEPEAFGVQRVTGVEDLVLHESGDVVEVGAIPITLIHTPGHTPGSQCFFVDGRL
                     VSGDTLFLEGCGRTDLPGGDPAQLYESLTQKLAVVPDDTVLYPGHLYSMEPSAPMGEV
                     RSHNYVFRPKTREQWLMMFGHD"
     gene            complement(1464..2705)
                     /locus_tag="VFX21_13505"
     CDS             complement(1464..2705)
                     /locus_tag="VFX21_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5097035.1"
                     /translation="MRALVFGVDPEPVDEPLPADANRLEQHLRATPMGLQDLPDPALI
                     GPDWVVLRTRLCGICGSDTKQVFMDTGGDWADASMTAFISFPQVLGHEVVADVVEVGP
                     EARGVEAGQRVLLQCWLSCGPRGITPMCPACEAGDYSLCWNFLDGRLAPGIHVGNSSD
                     ATGGFGELVPAHASMAIPVPDDVTDEIAVLADPFAVALHSITRTPPPPGGKVVVWGAG
                     ALGTSAIAILRALYPSVEICVVARTAAQQALASKLGAHTVVPSDQEPEAVVEALAAWS
                     GGVLRKPWGGLPIAHPAFIDVVYDTIGTGATLEAALRVMKPRGSISITGVNAPARFEW
                     SPWYFKEINIVGSNAFGVEKVDGVRKHAIEHYLDMVRDGRIDISPMLTHTFRLDQWRE
                     AFTTLARQDRTGAIKVAFDYR"
     gene            2704..3531
                     /locus_tag="VFX21_13510"
     CDS             2704..3531
                     /locus_tag="VFX21_13510"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5097036.1"
                     /translation="MVSTLPVVLHYSFVAAPERDIEAVRLSTSRFLRTVDELTDAQMH
                     DPCLLPGWSRAELVTHLARNADGFRGMAEATARGDVGVMYAGGIEQRAADIAAGRGVR
                     ATTVTADLRRAADGLAAAWRQFPGDAWDREGKLIQGTRTIRDSVFMRWRELELHHVDL
                     GLAYDSNEWPIGFVGRALDDAMCTLPARAKARRTDMSGRYRVEATDHGRAWDIVITRD
                     AVTVVAAEPGVAPDAVVSGWGCDLLAWLTGRRASAGTVTAAGDDLGALRLPTWFPFP"
     gene            3576..>3844
                     /locus_tag="VFX21_13515"
     CDS             3576..>3844
                     /locus_tag="VFX21_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003989198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5097037.1"
                     /translation="MTITYRTCHLCEATCGLELHHEGREITLIRGDRDDVFSHGFLCP
                     KGTAIKHLEADPDRLRTPMIRTGEEWREATWDEAFELIDERLTAIR"
BASE COUNT          639 a         1316 c         1299 g          590 t
ORIGIN      
        1 tcggcgtgcc gttggaagac gccgcgcagt tccatcagtg ggccgagcag atcaacaccg
       61 gcccgttgca gcccgaggcc gggcacgccg cgtcgcaggc gatggtcgac tacttgcgtc
      121 cgttggtgga ggagcggcgc gccaatcccg gcgacgactt cctctccgat ctcgtccacg
      181 ccgaggtcga cggcgaacag ctcaccgacg gcaagctcta cgggttcctc cggttgttgc
      241 tgcccgcggg ggccgagacg acgtaccgcg tgatgggcaa ctgcctgtac gcgctgctga
      301 cgcatcccga cgagatggcg cgcgtgctcg acgatcgcgc gctcattccc gacgtgatcg
      361 aagagacgtt gcggtgggag acgtcggtga cgatggtcag ccgcatcgcg acgaaggacg
      421 ccgaggtttc cggttgcccg atcaaggccg gctcgccggt gggtgtgctc accggttccg
      481 cgaaccacga cgaagatcgc tgggacgatg cgagcgagtg gaagctcggc cgcgcgacgc
      541 agcaccacat cgcgttcggg accggcccgc accagtgcct cggcatgcac ctggcgcgtc
      601 tggagttgcg agtggggctc gatgtgattc tcgaccgctt gcccaacctg cgcctggacg
      661 acggcgccga gggcgcgttc atcgagggct acgccttccg cggtccacgc gcactgcccg
      721 tcaccttcgg ctgaccgcgc cgccgcggcc acgtcgataa tcagtcgtgg ccgaacatca
      781 tcagccactg ctcacgggtc ttcgggcgga agacgtagtt gtgcgaacgc acttcaccca
      841 tcggcgccga cggctccatc gaatacaagt gcccgggata cagcaccgtg tcgtcgggga
      901 cgactgcgag cttctgcgtc aggctctcgt acaactgcgc ggggtccccg cccggcagat
      961 cggtgcgccc gcagccctcg aggaacagcg tgtcgcccga gaccaaccgg ccgtcgacga
     1021 agaagcactg cgaaccgggc gtgtgcccgg gcgtgtgaat cagcgtgatc gggatcgcgc
     1081 cgacctcgac gacgtcgccg ctctcgtgca gcaccaagtc ctcgacgccc gtcacccgct
     1141 gcacgccgaa tgcctccggt tcctgcacgt gaatgcgcgc gtccacgccc atctcgcgca
     1201 actccgccaa cccctcgatc gaccagccgc ccagtgaacc accgacgtga tcggggtgat
     1261 agtgcgtcac caacacgtcg gtgcaccgca aaccgtcggc gtcgagcagc tccaccagct
     1321 cgcgcacccc gtacgcggga tcgatcaacg cggcggaacc ggtttcgcga tcgccgatga
     1381 cgtacacgaa gttcaccatc tgctgtgcga tctggtcgtc gcgcgcgaag tcgcgaccgg
     1441 cgagcagttg gcggaaggag aacctatcgg tagtcgaagg caactttgat cgctcccgtg
     1501 cggtcttggc gcgccagtgt cgtgaacgcc tcgcgccatt ggtcgaggcg gaacgtgtgc
     1561 gtgagcatgg gcgagatgtc gatacgaccg tcgcggacca tgtcgaggta gtgctcgatc
     1621 gcatgcttcc gcacgccgtc gaccttctcg acaccgaacg cgttggagcc gacgatgttg
     1681 atctccttga agtaccacgg ggaccattcg aatcgcgccg gtgcgttgac gccagtgatg
     1741 gagatcgagc cgcggggctt catcacccgc aacgcggctt ccaacgtcgc gccggtgccg
     1801 atcgtgtcgt agacgacgtc gatgaacgcg ggatgcgcga tcggcaagcc gccccacggc
     1861 ttgcgcaaga cgccgcccga ccatgccgcc aacgcctcga cgacggcctc gggctcctgg
     1921 tcggagggca cgaccgtgtg cgcgcccaac ttggacgcca acgcttgttg cgcagccgtc
     1981 cgcgcgacga cgcagatctc gaccgacgga tacagcgcgc gcaggatcgc gattgcgctc
     2041 gtgcccaacg cacccgcgcc ccacacgacg accttgccgc ccggcggcgg gggcgtgcgc
     2101 gtgatcgagt gcaacgcgac ggcgaacggg tccgcgagca ccgcgatctc gtcggtgacg
     2161 tcgtcgggca cggggatcgc catcgatgcg tgcgcgggga cgagctcacc gaagccgccg
     2221 gtcgcatccg acgagttgcc gacgtggatg cccggcgcca gccggccgtc gaggaagttc
     2281 cagcacaacg agtagtcgcc ggcttcacag gccgggcaca tcggcgtgat cccgcgcggt
     2341 ccgcacgaca accaacactg caacagcacg cgttgtccgg cttcgacacc gcgcgcctcg
     2401 gggccgactt cgaccacgtc ggcgacgacc tcgtggccga gcacctgcgg gaaagagatg
     2461 aacgcggtca tcgacgcgtc ggcccagtcg ccgccggtgt ccatgaacac ctgcttggta
     2521 tcggagccgc agatgccgca caagcgcgtg cgcagcacca cccagtcggg gccgatcagt
     2581 gcgggatcgg gcaggtcttg gaggcccatc ggcgtcgccc gcaggtgctg ttcgagccgg
     2641 ttcgcatcgg ccggcaaggg ctcgtcgacc ggttcgggat cgacgccgaa cacgagtgca
     2701 cgcatggtgt cgacactacc ggttgtgctg cactattcgt tcgtggcagc gcccgagcgc
     2761 gacatcgagg ccgtgcgcct gtcgacgagc cgatttctcc gcaccgtcga cgagctgacg
     2821 gacgcgcaga tgcacgaccc gtgcctgctt cccggttggt cgcgcgccga gttggtgacg
     2881 cacctcgcgc gcaacgccga cggctttcgc ggtatggccg aagcaacggc gcgcggcgac
     2941 gtcggcgtca tgtatgccgg tggcatcgaa caacgcgcgg ccgacatcgc cgcgggccgg
     3001 ggtgttcggg cgacgaccgt gaccgcggat cttcgtcgcg cagccgacgg cctcgcggcg
     3061 gcatggcgtc agtttcccgg cgacgcgtgg gatcgggaag ggaagctgat ccagggcacc
     3121 cgcacgattc gtgattccgt cttcatgcgt tggcgggagc tcgagctgca ccacgtcgac
     3181 ctcgggctcg cgtacgactc gaacgaatgg ccgatcggct tcgtgggccg cgccctggac
     3241 gacgcgatgt gcacgctgcc ggcgcgggcg aaggcgcgtc ggaccgatat gagcggccgc
     3301 taccgcgtcg aggcaaccga ccacggccgg gcctgggaca tcgtgatcac gcgcgatgcc
     3361 gtcaccgtcg tcgcggccga gcccggcgtc gcaccggatg cggtcgtcag cggctggggt
     3421 tgcgacctgt tggcgtggct caccggccgc cgcgcgagcg caggcaccgt gaccgcggcc
     3481 ggcgacgacc tcggcgcgct gcggctgccc acgtggttcc cctttccctg acagggagag
     3541 gacctgaccg ccgaccaggg cttattgtcg ggggcatgac gatcacgtat cgcacgtgcc
     3601 acctgtgcga agcgacgtgc ggtctcgagc tgcaccacga aggacgcgag atcacgctca
     3661 tacgtggtga ccgcgacgac gtgttcagtc acggcttcct gtgcccgaaa ggcacggcga
     3721 tcaagcacct cgaagccgac cccgatcgtc tccgcacccc gatgatccgc acgggtgagg
     3781 agtggcgcga agcaacgtgg gacgaagcgt tcgagctcat cgacgaacga ctcactgcga
     3841 tccg
//
LOCUS       DASUGA010000678         3758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_622275, whole genome shotgun sequence.
ACCESSION   DASUGA010000678 DASUGA010000000
VERSION     DASUGA010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622275"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="VFX21_13520"
     CDS             complement(<1..1297)
                     /locus_tag="VFX21_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097038.1"
                     /translation="MATGGGAAPEEAAPSAPRIATWLDAFEEACSASRYDDAAAMLEE
                     QVLTLLGRGEHDTVATLIARLPVAARTPLLRAADADVSTRAHAIDLASLDALERRVQS
                     RIDLRAFVVAVKAQHLLWRGDPLCFSVAQSGLDLLPHDGLVSPVGLFARGRLRLVLAI
                     GTLFVSFAARASAEELLDDAVADFMRAGALAEAAAAQAYVHGLIAAVTWTDVADAAET
                     VGAAAARLQSRHSPYAPFALVSEALLRFIAGDMWSCHRVLESYDRWRGPRPEFVPFIA
                     DYVRLVAQLIGDGGAGDAFDGDAVRIVDGLDRHAPELAGTMTLTIAGVLTDIGDHSRA
                     RQWLARARQAPQPLPSDPLGRQLLEARLRLAHARGGEVDDAVTSGLEAVAGMREAGYG
                     RAADIALARLARSVRDAGYDARADGLRDAAAAALVPVQTV"
     gene            1402..2226
                     /locus_tag="VFX21_13525"
     CDS             1402..2226
                     /locus_tag="VFX21_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009297359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HEX5097039.1"
                     /translation="MTTDPILTRRRALGLLAGAGASAVVLAACGKSSSNDAADGASTT
                     AATTSGTTATDTTDSTSTSGASCDTAIPEETAGPYPGDGSNGPDALTTDGVVRRDITS
                     SFGGASGTAEGVPLTIELTIVDTTNNCAPLEGAAVYVWHCDREARYSMYTEGAEDQNY
                     LRGVQAADADGKLAFVSIFPAAYSGRWPHVHFEVYDSVADATGGGRPRATSQLALPKA
                     ACDKVYATSGYEDSVRNMARTSLDSDMVFADGYAAQLGTVTGDAENGMTVTLTVPV"
     gene            2305..2904
                     /locus_tag="VFX21_13530"
     CDS             2305..2904
                     /locus_tag="VFX21_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097040.1"
                     /translation="MEGTGATTTRAAAHAGWPGVGRFSSDPMWYPVSLYPVGEALLVH
                     RRRAGIIAEARRSIGGHVTVDEHVYGTLVTMYDRDGMVGRVLVGPRTSVVEDLRTRGW
                     IRGVAAGNRAYSCKVTTPGAARATRGFLEVEPAELRLTLGSSAPIVLRADEGAVVEGE
                     RNVRRVFLTLATPYRTHATIVVGRTDPLIAALQRTGWVD"
     gene            complement(3043..3495)
                     /locus_tag="VFX21_13535"
     CDS             complement(3043..3495)
                     /locus_tag="VFX21_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097041.1"
                     /translation="MQERTHVDAIWADRAQLCVARTVALPFTAAIARFVGHETPAGLA
                     LDPYWKRDNVGPLGRCHLASARTRGTLKVGGRAIPVELELTPWANRITEVVLRPASNR
                     AYRWGSRRRQRWYPAAHTAIDALRHELLATPQRPLTARPAPEQGRLAG"
BASE COUNT          561 a         1319 c         1348 g          530 t
ORIGIN      
        1 agaccgtctg cacgggcacc aacgccgcgg ccgcggcgtc gcgcagtccg tcggcgcgcg
       61 cgtcgtagcc ggcgtcgcgt accgaccgag ccaggcgtgc gagcgcgatg tctgccgcgc
      121 gcccgtatcc ggcctcgcgc atgccggcga ccgcttcgag accggatgtg accgcgtcgt
      181 cgacttcgcc accgcgcgcg tgtgcgagcc gtaaacgcgc ctcgagcagt tgccggccga
      241 gcggatccga ggggagcggt tgcggtgcct ggcgggcgcg cgccaaccac tggcgggcgc
      301 ggctgtggtc accgatgtcg gtcaacacac ccgcgatcgt cagcgtcatc gtgccggcca
      361 gctcgggtgc gtgccggtcg agtccgtcga cgatgcggac cgcgtcgccg tcgaacgcgt
      421 cgcccgcccc tccgtcaccg atcaactgcg cgaccaaacg cacgtagtcc gcgatgaacg
      481 gcacgaattc cggtcgcggc ccgcgccagc ggtcgtagct ctcgagaacg cgatggcagc
      541 tccacatgtc acccgcgatg aagcgcagca gagcttcgga cacgagcgcg aacggcgcgt
      601 acggggagtg gcgcgattgc agccgcgccg cggcggcacc gacggtctcg gccgcatcgg
      661 cgacgtcggt ccaggtgacc gcggcgatca agccgtgcac gtaggcctgc gcggcggcgg
      721 cctcggccaa cgcgcccgcg cgcatgaagt ccgcgaccgc gtcgtcgagc agctcctcgg
      781 ccgacgctcg tgccgcgaac gacacgaaca gcgtcccgat ggcgagcacg aggcggagcc
      841 ggccgcgcgc gaacaaaccg accggactca cgaggccgtc gtggggcaag agatcgaggc
      901 cggactgcgc gacggagaag cacaggggat cgccgcgcca taacaggtgt tgcgccttca
      961 ccgcgacgac gaacgcgcgc aggtcgatcc tcgactgcac ccgccgctcg agcgcgtcga
     1021 gcgacgccag atcgatcgcg tgcgcgcggg tcgagacatc cgcgtcggcg gcacgcaaca
     1081 gcggcgtccg ggccgcgacg gggagccgcg cgatcagcgt cgcgacggtg tcgtgttcgc
     1141 cgcggccgag cagggtgagc acctgctctt cgagcatggc cgccgcgtcg tcgtagcggg
     1201 acgcgctgca cgcttcctcg aaggcgtcga gccacgtcgc gatgcggggc gcggagggcg
     1261 cggcctcctc gggcgcggca ccgcctcctg tcgccactgc tccggccttc cgactgtccg
     1321 cccgccggaa aactacacgc ctcaggaagt tcggagcgcg ctcccaggcg acgcttgcca
     1381 tggtcatgca cactcgtagt catgacgacg gatcccatcc tcacccggcg ccgggcgctc
     1441 gggctgttgg ccggtgccgg cgcgagcgcg gtggtgctcg cggcgtgcgg caagagcagc
     1501 tcgaacgacg cggccgacgg cgcgtccacc accgcggcga cgacgtcggg cacgacggcg
     1561 accgatacca ccgacagcac gagtacgagt ggcgcgagtt gcgacaccgc gatccccgag
     1621 gagaccgccg gcccgtaccc gggcgacggt tccaacggcc cggatgcgtt gacgaccgac
     1681 ggcgtcgtac gccgcgacat cacgtccagc ttcggcggcg cgagcggcac cgccgaaggt
     1741 gtgccgttga cgatcgagct gacgatcgtc gacacaacga acaactgcgc gccgctggaa
     1801 ggcgcagccg tctacgtctg gcactgcgac cgcgaggctc gctattcgat gtacaccgaa
     1861 ggcgccgagg accagaacta tctgcgcggg gtgcaggccg cggatgccga cggcaagctg
     1921 gcgttcgtga gcatctttcc cgcggcgtat tccggtcgct ggccgcacgt gcacttcgag
     1981 gtgtacgact cggttgccga cgcgacgggc ggcggccgcc cgcgcgcgac gtcgcagctc
     2041 gcgctgccca aggcggcctg cgacaaggtc tacgcgacga gtggctacga ggacagcgtg
     2101 cgcaacatgg cgcgcacgtc gctcgacagc gacatggtgt tcgccgacgg ctacgccgca
     2161 cagctcggga cggtcaccgg tgatgccgag aacggcatga cggtcacgct gactgtgccg
     2221 gtctgaacgg gggcttgccc caggctgtac gatttgtccc agggcgacgg tgatgagacc
     2281 gagtcgcttg gggggaacga atccatggaa ggcaccggtg cgacgacgac tcgcgcggct
     2341 gcacacgcag gctggccggg cgtcggtcgc ttctcgtccg acccgatgtg gtaccccgtg
     2401 tcgctgtatc ccgtcggtga agcgctgctc gtgcaccgcc gccgggcggg gatcatcgcc
     2461 gaagcgcgcc gctcgatcgg cggccatgtg accgtcgacg agcacgtcta cggcacgctc
     2521 gtcacgatgt acgaccgcga tggcatggtc ggacgcgtgc tcgtcggccc ccgaacttcc
     2581 gtcgtcgaag acctccggac gcgcggttgg atccgcggcg tcgcggccgg caaccgagcg
     2641 tattcctgca aggtgacgac ccccggtgcg gcgcgcgcga cgcgcggctt tctcgaggtg
     2701 gagcccgcgg aactgcggct cacgctcgga tcgagcgcac cgatcgtgtt gcgcgccgac
     2761 gagggcgcgg tcgtcgaggg cgagcgcaac gtccgccgcg tcttcctgac cctcgccacg
     2821 ccgtaccgca cccacgcgac gatcgtcgtc ggtcgcaccg acccactgat cgcggcgtta
     2881 caacgcaccg gctgggtcga ctgacccacc gcgggtcttg acatcagaat cgattcggcg
     2941 cgggttcgcc gcacgttttc gaatttgatg gcgaaaggcg cgaggaagag ggcgctccac
     3001 ccggatcgcc cgccgggggg tgcgggcgat ccgagcgttg cgtcagccgg cgagccgtcc
     3061 ttgttccggc gccggacgcg ccgtcagcgg acgctggggt gtcgccagca gctcgtgacg
     3121 cagtgcgtcg atcgcggtgt gcgcagcggg gtaccaacgc tgccgccgcc gggaccccca
     3181 gcggtaggcg cggttggatg ccggccgcaa cacgacttcg gtgattcgat tcgcccaggg
     3241 tgtgagctcg agctcgacgg ggatcgcgcg gccgccgacc ttcagcgtgc cgcgcgtgcg
     3301 cgccgacgcg aggtggcagc ggccgagcgg gccgacgttg tcgcgcttcc agtacgggtc
     3361 gagcgccagc ccggcggggg tctcgtgccc gacgaagcgc gcgatcgcgg ccgtgaacgg
     3421 cagcgcgacc gtgcgggcca cgcacagctg cgcccggtcc gcccagatgg cgtcgacgtg
     3481 tgttctctcc tgcacgaggt acatgctcgc cgttccgtgt ttctgctgcg cgcccgccag
     3541 acgtcgcgcg gatgaatccg atgtggacag tcgctgactg tccgaattcg cccgtctcgg
     3601 cgttacgcct ccgtcaccgc gatgtcgcgg cgggttgcgt gccagagcaa ctcttcggcc
     3661 agctcgtcga gcgccgcgtt cgtgagttgc gcgagctcgc gtgtgcgttc ctgccaccag
     3721 ggcgtgagct cggtcgtcaa cagccgcagc aggcaccg
//
LOCUS       DASUGA010000679         2042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_66288, whole genome shotgun sequence.
ACCESSION   DASUGA010000679 DASUGA010000000
VERSION     DASUGA010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66288"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="VFX21_13540"
     CDS             <1..951
                     /locus_tag="VFX21_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097042.1"
                     /translation="GESPFGAIGVCDPPDPVGTALFHLAYDAPHAGLPATGSAAPDGA
                     YDAPERPLLDLRPTDVDGVTRTASNARAGTRVDDLVVDLIPQSNVDARVYVLGYANEG
                     ETRPCLREGGTGEAIAHIEALTKATLTIANLCAGSLYYVVAEITDALGAKSTYAPRGL
                     GMGTDDWDAVVATNHYRVHTNVTLTYSRTRVHREDLVHSNLTLGSLHVDFSNAGRSAT
                     DPVFGGLAADSSGVRGNLTTELYEKVDYAGRVTVARGSAARDACRIDARSDFVSGDAA
                     KQYDVERLLVDRVADLHIDIAGGPVFDYRVEILDQAVVPT"
     gene            complement(963..>2042)
                     /locus_tag="VFX21_13545"
     CDS             complement(963..>2042)
                     /locus_tag="VFX21_13545"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5097043.1"
                     /translation="DTVDVVIAATGVLHHPRDVEFEGLDSFRGAAFHSARWDHDVTVD
                     GRRVGIVGTGSTAVQLVSAVVPRVSHLCLFQRTAQWVIQQDNPAYTEEERNAYRDDDG
                     RLAQLHRDLSTAFGVFANAVVDADSQQAHMIAELCLANLENNVRDPELRERLRPQYRA
                     ACKRLVVSPDFYEAIQHPNAELVTEKIVRVEPEGVRTADDTLHELDVLVLATGFKADA
                     FMRPTQLIGRNGVTLDEVWAPRPTAYMSISIPGFPNFFMLNGPNGPVGNFSLIEVAEL
                     QFSYILQLVEQLRAGRCREVCATQAATDAHEAARVEASKKTIWVTGCRSWYLDDRGVP
                     AVWPWPFDRFRSEMAAPAPEAYELL"
BASE COUNT          334 a          705 c          692 g          311 t
ORIGIN      
        1 ggtgagagtc cgttcggcgc catcggtgtc tgcgacccgc cggatcccgt cggcaccgcg
       61 ctcttccacc tcgcgtacga cgcgccccac gcgggattgc ccgccaccgg atcggccgca
      121 cccgacggcg catacgacgc gcccgaacgg ccccttctcg acctgcgccc aaccgacgtc
      181 gacggcgtga cacgcacagc gtcgaacgcc cgagccggca ctcgtgtcga cgatctcgtc
      241 gtcgatttga tcccccagag caacgtggac gcgcgcgtat acgtgctcgg atacgccaat
      301 gagggggaga cccggccgtg cctacgcgag ggcggcacgg gagaggcgat cgcgcacatc
      361 gaagcgctga cgaaggcgac gctcaccatc gcgaacttgt gcgccggcag cctgtactac
      421 gtggtcgccg agatcaccga tgcattgggc gcgaagagca cgtacgcgcc acgcgggctc
      481 ggcatgggca ccgacgactg ggacgcggtc gtcgccacca accactaccg cgtgcatacg
      541 aacgtgacgc tgacctacag ccggacccgc gtgcaccgcg aggacctcgt gcattcgaac
      601 ctgacgctcg gctcgctgca cgtcgacttc tcgaacgccg gacgcagcgc gaccgacccg
      661 gtcttcggcg gcctcgccgc ggattccagc ggcgtgcgtg gcaacctgac caccgagctg
      721 tacgagaagg tcgactacgc cggacgcgtg acggttgcgc gcggcagtgc agcccgcgac
      781 gcgtgccgga tcgacgcgcg cagcgatttc gtcagcggtg acgccgcgaa gcagtacgac
      841 gtggagcggc tgctggtcga ccgcgtcgcc gacctgcaca tcgacatcgc cggcggaccg
      901 gtgttcgact accgcgtcga gatcctcgac caagcggtcg tcccgaccta gtgccccact
      961 ggtcagagga gctcgtacgc ttccggcgcg ggcgctgcca tctcggatcg gaagcggtcg
     1021 aacggccacg gccacacggc cggcacaccg cggtcgtcga ggtaccagct gcggcaaccg
     1081 gtgacccaga tcgtcttctt cgacgcctcg acgcgtgcgg cctcgtgggc gtcggtcgcc
     1141 gcctgggtcg cgcacacctc gcggcacctg cccgcgcgca actgctcgac gagctgcaag
     1201 atgtacgaga actggagttc tgcgacttcg atcagcgaaa agttcccgac gggtccgttg
     1261 ggcccgttca acatgaagaa gttcgggaag ccgggaatcg agatcgacat gtacgcagtc
     1321 ggtcgcggcg cccagacctc gtcgagggta acgccgttgc gcccgatcag ctgtgtcggc
     1381 cgcatgaacg cgtccgcctt gaagcccgtc gccagcacga ggacgtcgag ctcgtgcagt
     1441 gtgtcgtccg cggtgcgcac gccctccggt tcgacgcgga cgatcttctc ggtcacgagc
     1501 tccgcgttcg gatgctggat cgcctcgtag aagtcgggtg acacgaccag tcgtttgcac
     1561 gcggcccggt actgcggacg cagccgctcg cgcagttcgg ggtcgcgtac gttgttctcg
     1621 aggttggcga ggcacagctc cgcgatcatg tgcgcctgct gcgagtcggc gtcgacgacg
     1681 gcgttggcga agacgccgaa cgcagtcgag aggtcgcggt ggagctgcgc gagtcgaccg
     1741 tcgtcgtcgc ggtacgcgtt gcgctcctcc tcggtatacg cagggttgtc ctgttggatc
     1801 acccactgcg cggtgcgctg gaagaggcag agatgcgaga cgcgcggcac gaccgccgag
     1861 accagctgta ccgccgtcga cccggtcccg acgatgccga cccgccggcc gtcgacggtg
     1921 acgtcgtggt cccatcgcgc gctgtggaac gcggcaccac ggaacgagtc gaggccctcg
     1981 aactcgacgt cgcgcgggtg gtgcagcaca ccggtggcgg cgatgacgac gtcgaccgtg
     2041 tc
//
LOCUS       DASUGA010000680         5389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_484948, whole genome shotgun sequence.
ACCESSION   DASUGA010000680 DASUGA010000000
VERSION     DASUGA010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484948"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VFX21_13550"
     CDS             <1..295
                     /locus_tag="VFX21_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097044.1"
                     /translation="AGDMICRAIAGQPKRVSTPVGMMAQVTAAVAPDVQDRVLNLAYR
                     LFPDSAAAKGEASEQPSNRETFVPGTTAGARRGGGDLSLPARAMVQAFRGIHW"
     gene            complement(304..1272)
                     /locus_tag="VFX21_13555"
     CDS             complement(304..1272)
                     /locus_tag="VFX21_13555"
                     /inference="COORDINATES: protein motif:HMM:NF019603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX5097045.1"
                     /translation="MRIFYDDDADTSVLDGQQIAVVGYGNQGRSWALNLRDSGAAPIV
                     CVRRDASRDEAERDGFEAHELEAANDADVLCILVPDDVIPLLPLRPRNTALTIVASGY
                     TLAFDRLAIEGDLGMVAPRMLGPLVRECYVEGAGFITAVGVHRDVTGTAEARMLAVAK
                     AIGGLREGGMELTAMQEALLDLAVEQVLSPALAAVNNAFVATMLAHDIPLEAVITELV
                     LSGEVERTYGLLREVGYAVQSEYHSPTSQYGQLSRRGQYDHLDVRSKMEELTADIESG
                     RFADEWDAERDDGYPRLNALKEQHAGPGVREFEAELRRRLGPGARK"
     gene            complement(1283..2044)
                     /locus_tag="VFX21_13560"
     CDS             complement(1283..2044)
                     /locus_tag="VFX21_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019663319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEX5097046.1"
                     /translation="MMTNPAVDVSTISRIEHDEAMRITAVEFDRALALFRSLDAGDWV
                     KPTDCDLWDVRGVVLHLLGSAEANASLVEQLRQMYKGSRLKKTINSPYWWDGANEYQI
                     AKHAALADGDIAAAYAAITPKATETRTKLPKVVRSLPVLNLPEPVGRKPLGFLTDMGF
                     TRDVWMHRVDIRRATGKTMELTAEHDGRIVADIVADWSTTHADPFTLELDGPAGGHFT
                     RGSGGEHVRIDAVEFTRILSLRETGTGVLANPFPL"
     gene            2189..3001
                     /locus_tag="VFX21_13565"
     CDS             2189..3001
                     /locus_tag="VFX21_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097047.1"
                     /translation="MLREFLALFALVALYLSMSAAPAAAASDVGYDISYPQCGLPFPS
                     DAAFGIIGVNGGRAFSANPCLAEQLAWGKKLSAPVSLYANTGNPGPALSSRWPIGQMS
                     PRECYGDNPNSTACAYNYGWNAAKDSVSTAARASMAVNGVDEKNARRRVANVTWWLDV
                     EILNSWQTTSSVYGPTEQSKLNDTAALAGAVNALWDAGVDSVGIYSTQYQWNLITGGS
                     AYTHDWFSANPVWLAGHSANSAAGGCEKPSFTGGRVTMTQYLSNGYDANYLC"
     gene            complement(3009..4151)
                     /locus_tag="VFX21_13570"
     CDS             complement(3009..4151)
                     /locus_tag="VFX21_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006914857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEX5097048.1"
                     /translation="MRTSAARILTTHAGSLPRPPALARLHGQRSRGEPVDPAELTAAV
                     EDATTAVITAQVEAGIDIGNDGEQARESFFTYVQHRMTGFGGTSERKLMRDLVEHPDY
                     LELMQPRRDRTQVHLFGAPAAIGAVAYRDTTEVAAECDLVAGAPFAETFMTAASPGIV
                     TAAMQNQHYPSDGEYLRAVADALATEYRYIVDRGLLLQIDAPDLAMERHTSFADRPLA
                     DFVAWIEGVVGHINRALDGIVPERVRLHVCWGNYEGPHTHDVPLDDILDVLYGAHVGA
                     LVISMANARHAHEYECFARRPLPDGMNIVAGVIDTTSNYVEHPAVVAERIVRVVDAVG
                     DPTRVIAGTDCGFDTSAGLGDVAASLVWCKLRALREGADLASARVF"
     gene            4229..5380
                     /locus_tag="VFX21_13575"
     CDS             4229..5380
                     /locus_tag="VFX21_13575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX5097049.1"
                     /translation="MSSTTASTRPASAPSIDDLVAATPVSRDRAVDFLRALSIVVVVC
                     WHWVFSVTHWNDHGALTMPNPIGDVPGLWLLTWVLQIMPSFFFVGGFANSVAWAANRR
                     KGASTASFLRTRLTRLGTPTAVFAVVWTAFEVVARLAFPSYTGVFHWGFVVFVPLWFL
                     GVYSAVVALAPCTLRLHERAPYTTLAGLAGIVALVDVVRFDTGLDAVGFANVLVVFLF
                     VHQLGYFYGDGTFTRPGSRAPWVLALGGLATMILLTTFGPYPHSMVAIRGEGVSNMFP
                     PNLAVAALGLFQAGLALLLRPALNRWLAHRRPWKVVVSVNAVAMTIFTWHMTAYVAAI
                     ALVEVLGGTLGAQPTASWWFQRPLWLALPGVFLAGLLAIFSRYELRSRG"
BASE COUNT          751 a         1843 c         1890 g          905 t
ORIGIN      
        1 ggcgggcgac atgatctgcc gcgcgatcgc cggtcagccc aagcgggtgt cgacacccgt
       61 cggcatgatg gcgcaggtca ccgcggcggt cgcacccgac gtgcaagacc gcgtgctcaa
      121 cctcgcgtac cgcttgttcc ccgactccgc ggcggccaag ggcgaagcga gcgagcaacc
      181 gagcaaccgc gagaccttcg tccccggcac gacagccggc gcccgccgcg gtggcggcga
      241 cctgtcattg ccggcgcgcg cgatggtgca ggccttccgc ggcatccact ggtagtcacg
      301 cggctacttg cgggcgccgg ggccgaggcg gcggcggagc tcggcttcga actcgcgcac
      361 gcccggccct gcgtgctgtt ccttcagcgc gttgaggcgc gggtagccgt cgtcgcgttc
      421 ggcgtcccac tcgtcggcga agcggcccga ttcgatgtcg gcggtcagct cttccatctt
      481 cgagcggacg tcgaggtggt cgtactgacc gcgccgcgaa agctgtccgt actggctggt
      541 cggggagtgg tactccgact ggaccgcgta gccgacctca cgcagcaagc cgtaggtgcg
      601 ctccacctct ccggagagca cgagctcggt gatgacggct tcgagcggga tgtcgtgcgc
      661 gagcatcgtc gcgacgaacg cgttgttcac cgccgcgagc gcgggtgaga gcacctgctc
      721 gaccgcgaga tcgagcagcg cttcctgcat cgcggtcagc tccatcccgc cctcgcgcag
      781 gccgccgatc gccttcgcca cggcgagcat gcgcgcctcg gcggtgccgg tcacgtcgcg
      841 gtgcacgccg acggccgtga tgaagcccgc accctcgacg tagcactcgc gaacgagggg
      901 gccgagcatg cgcggcgcga ccatcccgag gtcgccctcg atcgcaagac gatcgaacgc
      961 gagcgtgtag ccgctcgcga cgatcgtcaa cgcggtgttg cgcggccgca acggcagcaa
     1021 cgggatcacg tcgtcgggca cgaggatgca caagacgtcg gcgtcgttcg cggcctccag
     1081 ctcgtgggcc tcgaagccgt cgcgctcggc ttcgtcgcgc gacgcgtcgc gccgcacgca
     1141 gacgattggc gccgcaccgg agtcgcgcag gttgagcgcc cacgagcgcc cctggttgcc
     1201 gtagccgacg acggcaatct gttgtccgtc gagtacagac gtgtcggcgt cgtcgtcgta
     1261 gaagatccgc atccgcggga cgctacagcg ggaacgggtt cgcgagcacg ccggtgccgg
     1321 tctcgcgcaa cgagaggatg cgcgtgaatt ccacggcgtc gatgcgcacg tgctcgcctc
     1381 ccgaaccgcg cgtgaagtgt ccgcccgcgg gcccgtcgag ctcgagcgtg aacggatcgg
     1441 cgtgcgtcgt cgaccagtcg gcaacgatgt cggcgacgat gcgaccatcg tgctcggctg
     1501 tcagttccat cgttttgccg gtcgcgcgtc tgatgtcgac gcgatgcatc cacacgtcgc
     1561 gcgtgaaccc catgtcggtg aggaagccga gcggcttgcg gccgaccggc tccggaaggt
     1621 tgaggacggg caacgatcgc accaccttcg ggagcttcgt gcgcgtctcc gtcgccttcg
     1681 gcgtgatcgc ggcgtacgcc gccgcaatgt cgccgtcggc gagcgcggca tgcttggcga
     1741 tctggtactc gtttgcgccg tcccaccagt acgggctgtt gatcgtcttc ttcaaccgcg
     1801 agcccttgta catctggcgg agctgctcca ccagcgacgc gttcgcctcc gcggaaccga
     1861 gcaagtgcag gacgacgccc cgcacgtccc agaggtcgca gtcggtgggc ttgacccagt
     1921 cgccggcatc gagggaccgg aagagtgcga gcgcgcggtc gaactcgacc gcggtgatcc
     1981 gcattgcttc gtcgtgctcg atgcgactaa tcgtcgagac gtctaccgca gggttcgtca
     2041 tcatcgggcg attttctcgc actcgcggaa gacgtcgcgt ccggcgaagc gccgcgtcct
     2101 gcggattctc tgcgtcgcaa ttgccgggct ccgtgggagg agtcaagctt tgtgacgggg
     2161 gtggggttcc gtcactggtg aggtttcgat gctccgggag ttcctggccc tgttcgcgct
     2221 cgtcgcgctg tatctctcga tgtcggcggc gccggccgcg gccgcgtccg atgtcggcta
     2281 cgacatctcg tatccgcaat gcgggctgcc gttcccgagc gacgccgcct tcggcatcat
     2341 cggcgtgaac ggcgggcggg cgttcagcgc gaacccgtgc ctcgccgagc aactggcgtg
     2401 ggggaagaag ctgtcggcgc cggtctcgtt gtacgcgaac acgggcaacc cgggtcccgc
     2461 gctctcgtcg cgctggccga tcgggcagat gagtccgcgc gagtgctacg gcgacaaccc
     2521 gaactcgacc gcgtgcgcct acaactacgg atggaacgcg gccaaggact ccgtgtcgac
     2581 cgcggcgcgc gcgtcgatgg ccgtgaacgg tgtcgacgag aagaacgcgc gccggcgggt
     2641 cgcgaacgtc acctggtggc tcgacgtcga gatcctcaac agctggcaga cgacttccag
     2701 cgtgtacggg ccgaccgagc agtccaagtt gaacgacacc gccgcgctcg cgggcgcggt
     2761 gaacgcgctg tgggacgcgg gcgtcgactc cgtcggcatc tactcgacgc agtaccagtg
     2821 gaacctcatc accggcggtt ccgcgtacac gcacgactgg ttctcggcga atccggtctg
     2881 gctcgcaggt cacagcgcga actcggcggc cggtggttgt gagaagccgt cgttcacggg
     2941 tggtcgcgtc acgatgacgc agtacctgag caacggctac gacgcgaact acctctgctg
     3001 acgcccgatc agaagacgcg tgcgctggcg aggtcggcgc cctcgcgcag cgctcgcagc
     3061 ttgcaccaca cgagcgaagc ggcgacgtcg ccgaggccgg ccgaggtgtc gaagccacag
     3121 tcggtgcccg cgatgacgcg tgtgggatcg ccgaccgcgt cgacgacccg cacgatgcgc
     3181 tcggcgacaa ccgccgggtg ctcgacgtag ttactcgtcg tgtcgatgac gcccgcgacg
     3241 atgttcatgc cgtcggggag cgggcgccgg gcgaagcatt cgtactcgtg cgcgtggcgc
     3301 gcgttggcca tggagatcac gagcgcgccg acgtgcgcgc cgtagagcac atcgaggatg
     3361 tcgtcgagcg ggacgtcgtg ggtgtgtggg ccttcgtagt tgccccagca gacgtgcagc
     3421 cggacgcgct cgggcacgat gccgtcgagc gcgcggttga tgtgtccgac gacgccttcg
     3481 atccaggcga cgaagtccgc gagcggacga tcggcgaacg acgtgtggcg ctccatcgcg
     3541 aggtcgggcg cgtcgatctg gagcagcaga ccccggtcga cgatgtagcg gtattcggtc
     3601 gccaacgcgt cggcaaccgc acgcaagtac tcgccgtcgc tcgggtaatg ctggttctgc
     3661 atcgcggccg tgacgatgcc cggtgacgcg gccgtcatga acgtctccgc gaacggcgcg
     3721 cccgcgacga ggtcgcactc ggccgcgacc tcggtggtat cgcggtaggc gacggcgccg
     3781 atcgcggccg gcgcaccgaa gaggtgcacc tgcgtgcggt cacggcgggg ctgcatcagc
     3841 tcgaggtagt cggggtgctc gacgaggtcg cgcatcagct tccgctcgct cgtcccgccg
     3901 aaaccggtca tccggtgctg gacgtaggtg aagaagctct cgcgcgcctg ctcgccgtcg
     3961 ttcccgatgt cgatgccggc ctcgacctgc gccgtgatca ccgcagtcgt cgcgtcctcg
     4021 accgcggcgg tcagctcggc cgggtcgacc ggttcgccgc ggctgcgctg cccgtgcagc
     4081 cgggccaggg ccggcggccg ggggaggctt ccggcgtggg tcgtgaggat gcgggcggcg
     4141 ctggtgcgca tgacccgagt ctgcccccag gcgcggatgg ggggacgacc cgatgccgcc
     4201 cggcgcctcg ggcccgcatc ctcgacccat gagctccacg actgcttcga cccggccggc
     4261 ctcggcgccc tcgatcgacg acctggtcgc cgcgaccccg gtctcgcgcg accgggcggt
     4321 cgacttcctg cgggccctca gcatcgtggt cgtcgtgtgc tggcactggg tcttctcggt
     4381 cacgcactgg aacgaccacg gcgcgctcac gatgccgaat cccatcggcg acgtgcccgg
     4441 tctctggctg ctcacttggg tgctgcagat catgccgtcg ttcttcttcg tcggaggctt
     4501 cgccaactcc gtggcctggg ccgccaaccg gcgcaagggc gcatcgaccg cgagcttcct
     4561 gcggacgagg ctcacgcggc tcggcacgcc caccgcggtg ttcgcggtgg tgtggaccgc
     4621 gttcgaagtc gtcgcccgcc tcgcgttccc gtcgtacacc ggcgtgttcc actggggctt
     4681 cgtcgtcttc gtgccgctgt ggttcctcgg tgtgtacagc gcggtcgtcg cactcgcgcc
     4741 gtgcacgctg cgcttgcacg agcgggcgcc gtacacgacg ctggccgggc tcgcgggcat
     4801 cgttgcgctg gtcgacgtcg tgcgcttcga caccggtctg gatgccgtcg gattcgcgaa
     4861 cgtgctggtc gtcttcttgt tcgtgcacca gctcggctac ttctacggtg acggcacgtt
     4921 cacgcggccc ggatcgaggg caccgtgggt gttggcgctc ggtggtctgg cgacgatgat
     4981 cctcctgacg acgttcggcc cgtacccgca ctcgatggtc gcgatccgcg gcgagggtgt
     5041 gagcaacatg ttccccccga acctcgcggt cgccgcgctc ggcctgttcc aagccggcct
     5101 cgcgttgctg ctgcgccccg cgctgaaccg gtggctcgcg caccgccgcc cgtggaaggt
     5161 cgtcgtgtcg gtgaacgcgg tcgcgatgac gatcttcacc tggcacatga cggcgtacgt
     5221 cgccgcgatc gccctcgtcg aagtgctcgg cgggacgctg ggtgcacaac cgacggcgag
     5281 ttggtggttc cagcgcccgc tgtggctggc cctccccggc gtcttcctcg ccggcctgct
     5341 cgccatcttc tcccgctacg agctccggag tcgcggctag catcgaaca
//
LOCUS       DASUGA010000681         3134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_485209, whole genome shotgun sequence.
ACCESSION   DASUGA010000681 DASUGA010000000
VERSION     DASUGA010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485209"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VFX21_13580"
     CDS             <1..639
                     /locus_tag="VFX21_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012409486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase
                     (decarboxylating)"
                     /protein_id="HEX5097050.1"
                     /translation="DRGYSLMIGGDDAAVARLEPVFKALAPGVDAAPRTPGKEGDLQA
                     GEEGWLHCGPSGAGHFVKMVHNGIEYALMGAYAEGLNILHHANAGKLGRAEFNAETTP
                     LREPEAYQYDIDVGAVAELWRRGSVISSWLLDLTALALHESGDLANFETRVSDSGEGR
                     WTVEAAVEVGVPAPLITTALYNRFTSRGASEFGDRLLSAMRYYFGGHGPTPA"
     gene            complement(764..2194)
                     /gene="zwf"
                     /locus_tag="VFX21_13585"
     CDS             complement(764..2194)
                     /gene="zwf"
                     /locus_tag="VFX21_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008215855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX5097051.1"
                     /translation="MGSPTLIVLGASGDLAARLLFPALLSLEYRERLEDLRIVGYARQ
                     EWSTEQFHENLRAAMDTHVGDVDPKYFDKFIDRIDFHGGDISVESLRALHDVIDGPAV
                     FYLALPPGVFADAAETIAKAGLADESQGWRRLVIEKPFGTDVESALALNEQLHRYWRE
                     DQIFRIDHFLGKETVQNILVFRFANRFLEPVLRAAHVDEIQITVAETIGVEGRSRYYD
                     GIGAIRDMIQNHLIQMMTIATLEPPALREPEMLRDHKVEILKAVRTVDPDRDAVRGQY
                     TAGLIDGKPVIGYRGEPGVDPASRTETFAAVRLHLDTWRWEGVPVIMRSGKRLGSKAH
                     EVAIRFREPPTRLFRHTPLEHAEPNWLVFRMSPNECIDLVVRTKQPGLELEAHESVLR
                     ADYAEADDREASAYEGLLLDVLEGDHTPFLRFDEVEWAWRIVEPILQAWKVGAPEDYV
                     AGSDGPTGQHRFLDPGHAWRPIVPRT"
     gene            2224..>3134
                     /locus_tag="VFX21_13590"
     CDS             2224..>3134
                     /locus_tag="VFX21_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020659863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX5097052.1"
                     /translation="MTLKLGINLGYQDWANGLPKAIEIAQRAESLGFDSAWTAEAYGT
                     DAVTPLTWLMAHTERMSFGPAIMQMPARTPAMTAMTAATLDGMSGGRLRLGLGASGPQ
                     VAEGWHGQPYGKPLVRTREYVDIVREILRREKPLEHHGEHYDIPYTGAGATGLGKPLK
                     IIVHPRRADIPIYLAAIGPKNVELAAEIADGWLPIFYSPFRAKDAFGAALDAGFAKAG
                     GGKSLENFDVAPTVSVIVGDDPEMLANFVKPMLALYVGGMGARGKNFYNDLACRYGFE
                     KEAKEVQDLYLDGKKKEAADAIPFEMV"
BASE COUNT          528 a         1057 c         1062 g          487 t
ORIGIN      
        1 gaccgcggct actcgctgat gatcggcggc gacgacgcgg cggtcgcgcg cctcgagccg
       61 gtcttcaagg cgctcgcccc gggcgtcgac gcggcgccgc gcacccccgg caaggagggc
      121 gacctgcagg ccggcgagga gggctggctg cactgcggcc cgtccggcgc cgggcacttc
      181 gtgaagatgg tgcacaacgg catcgagtac gcgctgatgg gcgcctacgc agaagggctg
      241 aacatcctgc accacgcgaa cgccggcaaa ctcggccgcg ccgagttcaa cgcggagacg
      301 acgccgctgc gtgaacccga ggcgtaccag tacgacatcg acgtcggcgc ggtcgccgag
      361 ctgtggcgcc ggggcagcgt catctcctcg tggctactcg acctgaccgc gctcgcgttg
      421 cacgagtccg gtgacctcgc caacttcgaa acgcgcgtca gtgactccgg cgaaggccgc
      481 tggacggtgg aggccgccgt cgaagtgggc gtccccgcgc cgctgatcac gaccgcgctc
      541 tacaaccggt tcacgtcacg gggcgcgtcg gagttcggcg atcgcctgct gtccgcgatg
      601 cgctactact tcggaggtca cggaccgact cccgcctgac gggccgtcct cgtcgtcggg
      661 atggtcgacg cgaaacgtca gggcaacgcc gaccgccaag cacacggcga gtgcgaccag
      721 gacgatcgca agcacgacgc gccgacggcg atccatcgca cccttacgtg cgagggacga
      781 tgggccgcca cgcgtgaccg gggtcgagga accggtgctg accggttgga ccgtcgctgc
      841 ccgccacata gtcctcgggc gcgccgacct tccacgcctg caagatcggc tcgacgatgc
      901 gccacgccca ctcgacctcg tcgaagcgca agaacggcgt gtggtcgcct tcgagcacgt
      961 cgagcagcag accttcgtac gccgacgctt cgcggtcgtc ggcttcggcg tagtcggcgc
     1021 gcaacaccga ctcgtgcgcc tcgagctcga ggcccggttg cttggtgcgc acgacgaggt
     1081 cgatgcactc gttggggctc atgcggaaca cgagccagtt cggctcggcg tgttcgagcg
     1141 gtgtgtgccg gaagaggcgc gtcggcggct cgcggaagcg gatcgcgacc tcgtgcgcct
     1201 tcgacccgag gcgcttgccg gatcgcatga tgacgggcac accttcccag cgccacgtgt
     1261 cgaggtggag ccgcactgcc gcgaacgtct cggtccgcga cgcggggtcg acaccgggct
     1321 cgccgcggta gccgatgacc ggcttcccgt cgatgagtcc cgccgtgtac tgcccgcgca
     1381 ccgcgtcccg atcgggatcg acggtgcgca ccgccttgag gatctcgacc ttgtggtcgc
     1441 gcagcatctc gggctcgcgg agcgcgggcg gttcgagcgt cgcgatcgtc atcatctgga
     1501 tcaagtggtt ctggatcatg tcgcggatcg cgccgatgcc gtcgtagtag cgcgaccgtc
     1561 cttcgacccc gatcgtctcc gcgaccgtga tctggatctc gtcgacgtgc gcggcgcgca
     1621 acaccggttc gaggaaacgg ttcgcgaacc ggaacacgag gatgttctgc accgtctcct
     1681 taccgaggaa gtggtcgatg cggaagatct ggtcctcgcg ccagtaacgg tgcagctgtt
     1741 cgttcagcgc gagcgcggac tcgacgtcgg tgccgaaggg cttctcgatg acgagccggc
     1801 gccatccctg cgattcgtcg gcgagacccg ccttcgcgat cgtctccgcg gcgtccgcga
     1861 acacaccggg cggcagcgcg agatagaaga ccgccggccc gtcgatgacg tcgtgcaggg
     1921 cgcgcagcga ctcgaccgag atgtcgccgc cgtggaagtc gatccggtcg atgaacttgt
     1981 cgaagtactt cgggtcgacg tcgcccacgt gcgtgtccat cgcggcgcgg aggttctcgt
     2041 ggaactgctc cgtcgaccac tcctggcgcg cgtacccgac gatgcgtagg tcttcgagtc
     2101 gctcgcggta ctccaacgag aggagcgccg ggaacaacaa gcgcgccgcg agatcgccgg
     2161 acgcaccgag cacgatgagt gtcggactac ccatggtctg atcgtacgtg ccacgattcg
     2221 gacatgacgc tgaagctcgg gatcaatctc ggataccagg actgggccaa tggcctaccc
     2281 aaagcgatcg agatagccca acgagccgaa tcactcggtt tcgattcggc gtggacggcc
     2341 gaggcctacg gcaccgacgc cgtgacaccg ctgacatggt tgatggcgca tacggaacgg
     2401 atgtccttcg gccccgcgat catgcagatg ccggcccgga cccccgcgat gaccgcgatg
     2461 accgcggcca cgctcgacgg catgagcggt ggccgcttgc gcctcggcct cggtgcctcg
     2521 ggtccgcagg tcgccgaagg gtggcacggg cagccctacg gcaagccgct cgtccgcaca
     2581 cgtgaatacg tcgacatcgt gcgcgagatc ctgcggcgcg agaagccgct cgagcaccat
     2641 ggtgagcact acgacattcc gtacaccggc gcgggcgcga ccggtctcgg gaagccgctg
     2701 aagatcatcg tgcatccgcg ccgcgccgac atccccatct atctcgcggc catcggcccg
     2761 aagaacgtcg agctcgcggc cgagatcgcg gacggctggt tgccgatctt ctactcgccg
     2821 ttccgcgcca aggacgcgtt cggcgccgcg ctcgacgcgg gattcgccaa ggccggcggc
     2881 ggcaagtcgt tggagaactt cgacgttgcg ccaaccgtgt cggtcatcgt cggcgacgat
     2941 cccgagatgc tcgcgaactt cgtcaagccg atgctcgcgc tgtacgtcgg cggcatgggc
     3001 gcgaggggca agaacttcta caacgacctc gcgtgccgtt acggattcga gaaggaagcc
     3061 aaggaagtgc aagacctgta cctcgacggc aagaagaagg aagccgccga cgcgatcccc
     3121 ttcgagatgg tcga
//
LOCUS       DASUGA010000682         5011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1977700, whole genome shotgun sequence.
ACCESSION   DASUGA010000682 DASUGA010000000
VERSION     DASUGA010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1977700"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..568
                     /locus_tag="VFX21_13595"
     CDS             203..568
                     /locus_tag="VFX21_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019992803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5097053.1"
                     /translation="MASRFYTVVVDAQDPATLARFWAAVLDWDVVFEGPEEVLVAKDE
                     NTYPGLGFVLVPEGKTVKNRLHIDLAPDDRDAEVERIIALGATRADVGQGDGQTWVVL
                     ADPEGNEFCVLRARDGGMR"
     gene            complement(565..1089)
                     /locus_tag="VFX21_13600"
     CDS             complement(565..1089)
                     /locus_tag="VFX21_13600"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5097054.1"
                     /translation="MTVRPARLPDDAAAIAGIDTSFDTDTIYDIMYDQDVFRLATAHA
                     SPPVTKRFPLDDLGDGHFWHEGHVATDDRGAVVGFLATRDERWNQRLIIWHCYVDRAA
                     RGGGHGYALMQTALDGGRARGLKLAWLETSNLAYPSVQWYRRQGFEVCGLDTTRYKHS
                     YAPGETSLFLARPL"
     gene            complement(1094..2287)
                     /locus_tag="VFX21_13605"
     CDS             complement(1094..2287)
                     /locus_tag="VFX21_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5097055.1"
                     /translation="MRLQYAPEDEQFRADLVAWLEANFPREAAENEPKLSSAHLTDWS
                     RRWQRTLFDNGWLVPGWPPELGGRNATPTQQMIYFEETNRREIPRSGNPQGLGIIAPS
                     LKDYGSEEQQQKFLLPTLRAEISWCLGMSEPNAGSDLASLKTRAEPDGDEFVVNGQKV
                     WTSGAHHADWCLCFVRTDPTAPKHKGITAFIIDTTSPGVERRPFAELSDQEYTDFNEV
                     FFNDVRVPKENMLGPLNGGWPITQGSLAHERAMLWITHAYDVQRVVTALTQLGDRITP
                     DGTRLGDDARFRDGVAGFYVDAQALLTMGYRGFSKFLHGKAAPEHSLLKLYGSEALQN
                     ALLFGAEAVGPAGYDADDLGPRMWRDGSWVIQYLRSFAATIPGGTSEIQRNIIAERVL
                     GLPRR"
     gene            2315..3277
                     /locus_tag="VFX21_13610"
     CDS             2315..3277
                     /locus_tag="VFX21_13610"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX5097056.1"
                     /translation="MDFEFSPEQEMLRDSVRRFLADRSSLSSARDGSVDPGVWAGLVA
                     LGADGLGMVDSAVVLEELGRAVCNVPLASQLAASVFGVSDGTLAVYEPGARYSWRTPA
                     TKVVGSGLHGAKAHVADVGSVASYCVTAVDGVFVTDAGIACTSRTVDGSRSFGTVTFD
                     GAPAQQLDAGPDDVQRALDRLGVAFVVDGVGAAARALELAVEYAKERVQFDKPIGSFQ
                     AVQHLCADMLRMVELARAAAYYACWALDEADPAEAHRAAVMAQAYAASAFPQLGANAI
                     QVFGGIGFTWEHDIHLYYKRLLSASTVTGTADDHLAELARITID"
     gene            3535..>5011
                     /locus_tag="VFX21_13615"
     CDS             3535..>5011
                     /locus_tag="VFX21_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097057.1"
                     /translation="MLSGLVPAANAVEPASAPGIVGKAREVRTTWTDEFGVSRPTGVT
                     YVPQRNEFLVAEASGASTQVLRLDRDENPQGELELPAVSDPSTLAFDAATNLLTAVSG
                     DELVVAPGSAITTARPPVERKDISDLGLQDATSATFDAASGTWFVLDAGGPALVTVPE
                     VAEDPGSGERLPAPALAGKDLQGIAFNPSDGLVYVGSADGLLIGLDGSGSVAKTYSLD
                     GIDLRDPRAMTFAPSTDSTDATETQSLFIADAGDQTRLGGVTEVTLAAVTALDVPVDA
                     GTLVQTIDTSAFSPGSPDPSGVTYVPAADRLWICDSEVEEVTGAGYHGVNLWQITRSG
                     AVTDTGTTYPAVSKEPNGLGYIAASNTLLISDDSLRRIHLVRPGPDGRFGTSDDIVTF
                     INTLAYGSDDTEDPTLDPVSGHIFFLDGVDTEVFRIDPVNGVFGDGNDVTTHFDVGQY
                     GITDTEALEYYPPHDSLIVGNRTGRSLIEVTKTGELLRTIDL"
BASE COUNT          761 a         1651 c         1766 g          833 t
ORIGIN      
        1 agccgagccc gacgaggaac gtcgcgtcct gactcgcgtc atccgacacc aacacgtcgc
       61 tgatcgaagg tgcgaaccag cgtgggatgc ggtcgagcac ctcgtgcagc gtcgccgccg
      121 cgttgtaggc gacgacgacg atgccgatcc ggtccttcac cggcccgacg gtaccagttg
      181 ggttttcggg tagaacgacc tcatggccag ccgcttctac acagttgtcg tcgacgcgca
      241 ggacccggcg acgctcgccc gcttctgggc cgcggtcctc gactgggacg tcgtgttcga
      301 aggacccgag gaggtcctcg tcgccaagga cgagaacacg taccccggcc tcgggttcgt
      361 gctcgtgccc gagggcaaga ccgtcaagaa ccggctgcac atcgacctcg cgccggacga
      421 ccgcgacgcg gaggtcgaac gcatcattgc actcggtgcg acgcgcgccg acgtcgggca
      481 gggcgacggc cagacgtggg tggtgctcgc cgatcccgaa ggcaacgagt tctgcgtgtt
      541 gcgcgcccgc gacggtggga tgcgctagag aggacgcgcg aggaagagcg acgtctcgcc
      601 cggcgcgtag ctgtgtttgt agcgcgtcgt gtcgagtccg cacacctcga atccctgccg
      661 gcggtaccac tgcaccgacg ggtacgcgag attgctggtc tccagccacg cgagcttcag
      721 gccgcgggcg cgtccaccgt cgagcgcggt ctgcatgagc gcgtacccgt ggccgccacc
      781 gcgagcggcg cggtcgacat agcagtgcca gatgatcagg cgctggttcc agcgctcgtc
      841 gcgcgtcgcg aggaagccga cgacggcgcc ccggtcgtcg gtcgcgacgt ggccttcgtg
      901 ccagaagtgg ccgtcgccga gatcgtcgag cgggaagcgc ttggtaaccg gcggcgacgc
      961 gtgcgcggtg gcgagccgga agacgtcttg gtcgtacatg atgtcgtaga tcgtgtcggt
     1021 gtcgaacgac gtgtcgatcc ctgcgatcgc cgccgcgtcg tcgggcaggc gcgcgggacg
     1081 aaccgtcatc gcgctagcgg cgcgggagcc cgagcacgcg ctcggcgatg atgttgcgct
     1141 ggatctcgct cgtcccaccg gggatcgtcg ccgcgaacga gcgcaagtac tggatgaccc
     1201 agctgccgtc gcgccacatg cgcgggccga ggtcgtcggc gtcgtagccg gcgggaccga
     1261 ctgcttccgc accgaacagc aacgcgttct ggagcgcttc actgccgtag agcttcaaca
     1321 aggagtgctc gggcgcggcc ttgccgtgca gaaacttcga gaagccgcgg tagcccatgg
     1381 tgagcaacgc ctgcgcgtcc acgtagaagc ccgccacgcc gtcgcggaag cgggcgtcgt
     1441 cgccgagccg tgtgccgtcg ggggtgatgc ggtcaccgag ttgcgtgagc gcggtgacga
     1501 cgcgctggac gtcgtacgcg tgggtgatcc acagcatcgc ccgttcgtgt gcgagcgaac
     1561 cctgcgtgat cggccagcca ccgttcagcg ggccaagcat gttctccttc gggacgcgca
     1621 cgtcgttgaa gaacacttcg ttgaagtcgg tgtactcctg gtcggacagc tccgcgaacg
     1681 gccgtcgctc cacgcccggc gacgtggtat cgatgatgaa cgcggtgatg cccttgtgct
     1741 tgggtgcggt cgggtcggtg cgcacgaagc acaggcacca gtcggcgtgg tgcgcaccgg
     1801 acgtccagac cttctggccg ttcaccacga actcgtcgcc gtcgggttcg gcgcgcgtct
     1861 tgagactcgc gagatcgctg cccgcgttcg gttcgctcat gccgaggcac cacgagatct
     1921 cggcccgcag ggtcggcagc aggaacttct gttgctgctc ctcgctgccg tagtccttga
     1981 gtgacggggc gatgatgccg aggccctgcg ggttgccgct gcgcggaatc tcgcggcggt
     2041 tggtctcctc gaagtagatc atctgctgcg tcggcgtcgc gttgcggccg ccgagctcgg
     2101 gcggccagcc cggcaccagc catccgttgt cgaacaacgt gcgctgccag cggcgcgacc
     2161 aatcggtgag gtgcgcgctc gagagcttcg gctcgttctc cgctgcttcg cgcgggaagt
     2221 tcgcttcgag ccacgcgacg agatcggcgc ggaactgttc gtcctcgggt gcgtactgca
     2281 gccgcatcgg gccggagagt agtttccggc gccgatggac ttcgagttct cgcccgagca
     2341 ggagatgttg cgcgattcgg tgcggcgctt ccttgccgac cgttcgtcgc tgtcgtcggc
     2401 gcgggacggc tcggtcgatc ccggagtgtg ggccgggctc gtcgcgctcg gcgccgacgg
     2461 gctcggcatg gtcgacagcg cggtcgtcct ggaggagctc ggccgcgcag tatgcaacgt
     2521 gccattggcc tcgcagttgg ccgcgtccgt gttcggcgtg tccgacggga cgctcgcggt
     2581 gtacgaaccc ggtgctcgct attcgtggcg cacgccggcc acgaaggtgg tcggtagcgg
     2641 gctgcacggc gccaaggcgc atgttgccga cgtgggctcg gtcgcgtcgt attgcgtcac
     2701 cgcagttgat ggcgtgttcg tcaccgacgc ggggatcgca tgtacgtcgc ggaccgtcga
     2761 cggttcacga tcgttcggca cagtgacgtt cgacggtgcg ccggcccaac agctggatgc
     2821 cggccccgat gatgtgcagc gcgcactcga ccgcctcggt gtcgcgttcg tcgtggacgg
     2881 tgtcggcgcc gcggcgcgcg cgttggagct cgcggtcgag tacgcgaagg agcgcgtgca
     2941 gttcgacaag ccgatcgggt ccttccaagc cgtgcaacac ctgtgcgcgg acatgctgcg
     3001 gatggtcgag ctggcgcgcg cggcggcgta ctacgcgtgc tgggcgctcg acgaggccga
     3061 cccggcggag gcgcaccgcg ccgcggtcat ggcgcaggcg tacgcggcga gtgcgttccc
     3121 acaactgggt gcgaacgcga tccaggtctt cggcggcatc gggttcacgt gggagcacga
     3181 catccacctg tactacaagc gcctcctgtc ggcgtcgacg gtcaccggca ctgccgacga
     3241 ccacctcgcc gaactcgcgc gcatcaccat cgactgacac gcgcgccggc gggcgtttgt
     3301 ccgatcagca cgttcggtgc cgtcggacgc gcgttgccga gctcacggca agcccgccgc
     3361 gtacgccatc ggcagtagaa ggaccgcccc atcggcgtga tccgccgccg gtctggtctc
     3421 cgtatcgtca cgtccactga ggtgcaccga tcgcgacctc ggggcggaag ggtgatacgg
     3481 atgctgcgcg gttctggtgc acgtaggttg tcgcttgtcg gtggtatcgc cgtagtgctc
     3541 agcggcctcg ttccggctgc gaacgcggtc gagccggcgt cggcgcccgg catcgtgggc
     3601 aaggcccgcg aggtgcgcac gacgtggacg gacgagttcg gcgtgtcgcg cccgactggg
     3661 gtgacctacg tcccgcagcg caacgagttc ctcgtcgccg aagcatcggg cgcgagcact
     3721 caggtcttgc gtctcgatcg ggacgagaac ccgcagggcg agctcgaact gcccgcagtc
     3781 tccgacccgt cgacgctggc gttcgacgcc gcgacgaatc tgctcaccgc ggtgagcggg
     3841 gacgagctcg tcgtcgcgcc gggttcggcg atcaccaccg cgcgtccgcc cgtggagcgc
     3901 aaggacatca gcgatctcgg gcttcaggat gcgacgagcg cgacattcga cgcggcatcc
     3961 gggacgtggt tcgtgctcga cgcaggcggt ccggcgctcg tcaccgtgcc tgaggtggcc
     4021 gaggaccccg gaagcggcga gcggctcccg gcccccgcgc tcgcggggaa ggacctccaa
     4081 gggatcgcgt tcaatccgtc ggatggactc gtctatgtgg ggagtgccga cgggctcctc
     4141 atcggactcg acgggtcggg aagcgttgcg aagacgtaca gcctggacgg catcgatctg
     4201 cgcgatccgc gggcgatgac gttcgcgccg agcaccgact ctaccgacgc gaccgagacg
     4261 caaagtctgt tcatcgccga tgccggcgac cagacgcggc tcggcggcgt cacggaggtg
     4321 acgctggcgg cggtgaccgc gctcgacgtt cccgtcgatg ccgggacgct cgtgcagacg
     4381 atcgatacat ccgcattcag tccggggagc ccggacccgt ccggtgtcac gtacgtcccc
     4441 gcggccgatc ggctctggat ctgcgactcc gaggtcgagg aagtgacggg cgcggggtac
     4501 cacggcgtca acctgtggca gatcacgcgg tcgggagccg tgactgatac cggcacgacg
     4561 tatccggcgg tgtcgaagga accgaacggc ctcgggtaca tcgcagcgag caacacgttg
     4621 ttgatctccg acgacagcct gcggcgtatc catctcgtgc gccccggtcc cgatggccgg
     4681 ttcgggacga gtgacgacat cgtgaccttc atcaacacct tggcgtacgg cagcgacgac
     4741 accgaggacc cgacactcga tcccgtgtcc ggtcacatct tcttcctcga cggtgtcgac
     4801 accgaggtgt tccggatcga tcccgtgaac ggcgtgttcg gtgacggcaa cgacgtcacg
     4861 acgcacttcg acgtcggcca gtacggcatc accgacaccg aagcgctcga gtactacccg
     4921 ccccacgact cgcttatcgt cgggaaccgc accggcagga gcctcatcga ggtcacgaag
     4981 accggtgagt tgctccgcac gatcgacctc c
//
LOCUS       DASUGA010000683         2810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1891055, whole genome shotgun sequence.
ACCESSION   DASUGA010000683 DASUGA010000000
VERSION     DASUGA010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891055"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            442..1608
                     /locus_tag="VFX21_13620"
     CDS             442..1608
                     /locus_tag="VFX21_13620"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEX5097058.1"
                     /translation="MISQPKVLVKEVHVWVDDAGNVYDHAVPGAKEQITYERELAQRR
                     ETIEDSIDEKLAEEAASGVEIGVNNVTTLQFAHQTKGPDAVADGHAYGLSAADITFAA
                     NSAALNTEFYADLVGIGGSPPEQEIDALNLLNGQTARLSNNQLSVREAWLRTELAHQR
                     LGVSIGRIDVTTHFDRNAAANDETTQFISDALVNNPVLGLASNGFGFVSEWDPKKSWN
                     FKFGMQQSTDPTNQLGSLSDSMFKLAEFEYIARPFGLPEGHYRVWARSDNSGGLGYRD
                     AYGVSIDQKVGQAITLFARYGNGEVGDIPGKEFFYSGGVEFGAPHTFNPGDFWGVGLA
                     HTDFEQGSSEKLAEVFYNLHLTTHLRSSFMLQYVEESATGEGYLLPGARIQVVF"
     gene            1677..>2810
                     /locus_tag="VFX21_13625"
     CDS             1677..>2810
                     /locus_tag="VFX21_13625"
                     /inference="COORDINATES: protein motif:HMM:NF014312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HEX5097059.1"
                     /translation="MALAGPALAQAPAAPHNMANMPPPASGAKVIVSDPTQVVMIVDR
                     DSNDIAFMDIKTKQILGRTFLGNNVNPHMVMMSPDGRYVVTGGQRSNTAYIIDSRTLE
                     LVAKIPVGFAPEHLAFSPDGRWYYQGNPEGDSITVIDMQSLKAVKTITGLHEPLNITF
                     TPDGAKAYIGNYGAHWVGVVDVTRHELIKRINIDTVPGVARLDPAKYLTEIKGIFNAT
                     PTPDGRFLYAADGDLGVVGVIDTREDKVIKVIRVGPDPWRVYMSHDGKYGISVNNGDS
                     TVSIIDIAKNAVVATYPVGPDLTGINFAAGKAFVISSTTGFLYVFDMATLKPAGRIKI
                     GNNLFLQTATTDTADQKLYLGSATDNSVYIIDAATEAVERVANV"
BASE COUNT          552 a          954 c          848 g          456 t
ORIGIN      
        1 ttaacgcacg ggttagcgcg gctctttaac gtccgatcaa cggtaaatag cgggagttaa
       61 tcccgctcgg ccggccatca ttcccctcgc ttttcggcgc cccgccgcgc ccggctacgc
      121 tcggacgttt ctcaaggttc caacgtcgcc gggcttggat cggtgcccga cgttccgtca
      181 aatacgagga aaaactagat gcgcacgccg taccgctcct tgccgtggcc cgcccgcaag
      241 gcattggccg ctgcaattgc cacggggttg gcaatcgccc tgcccgtcac cgctcaagct
      301 cagcgacgga acgtcgctct cccgccgccg acgccgaccc cggcgcccgc ggccgcaacg
      361 tccgcggacc ccaataccca agcgctgctc gatcgcatcg acgagctcga aaggcgcctg
      421 gccacgctcg agcagacggc cgtgatctcg caaccgaagg tgctcgtgaa ggaggtccac
      481 gtgtgggtgg acgacgccgg caacgtctac gatcacgcgg tacccggcgc gaaggagcag
      541 atcacctacg agcgcgagct cgcccagcgc cgggagacga tcgaggactc gatcgacgaa
      601 aagctggccg aggaagccgc gagcggcgtc gagatcggcg tcaacaacgt cacgaccttg
      661 cagttcgcgc atcaaacgaa gggccccgac gcggtggccg acgggcacgc ctacggcctg
      721 tcggccgcgg acatcacctt cgccgcgaac tcggccgcgt tgaacaccga gttctatgcg
      781 gacttggtcg gcatcggcgg ctcgcctccg gagcaagaga tcgacgcgct gaacctgctg
      841 aacggccaga ccgctcgttt gagcaataac cagctgagcg tgcgcgaggc gtggctccgc
      901 accgagctcg ctcatcagcg gctcggcgtg agcatcggcc gcatcgacgt caccacgcac
      961 ttcgaccgca acgcggccgc gaacgacgag acgacgcagt tcatcagcga cgccctcgtc
     1021 aacaacccgg tgctcggcct cgcgagcaac ggcttcggct tcgtgtcgga gtgggacccg
     1081 aagaagagct ggaacttcaa gttcggcatg cagcagagca ccgaccccac caaccagctc
     1141 ggatcgctgt ccgactcgat gttcaagctc gccgagttcg agtacatcgc gcggccgttc
     1201 ggcttgcccg aagggcacta tcgggtttgg gcgaggtccg acaatagtgg cggtctcggc
     1261 taccgcgacg cttatggtgt cagcatcgat cagaaagtcg gccaggcaat cacgctgttc
     1321 gcgcgctatg gcaacggcga ggtcggcgac atcccgggga aggagttttt ctacagcggc
     1381 ggcgtggagt tcggcgcccc gcacaccttc aatcctggag atttctgggg cgtcggcctc
     1441 gctcacaccg acttcgagca aggatcgagc gagaagctcg ccgaggtctt ctacaaccta
     1501 cacctcacga cgcacctcag atcgtcgttc atgctccaat atgtcgaaga gtctgcgacc
     1561 ggcgagggtt acctcctgcc gggcgcgcga attcaggttg tgttttgaca cgccctgcgg
     1621 agatcaccat gcgtaacatc aatcgacttc tcagcgtctc ccttaccgcc tcgttggtgg
     1681 cgctggccgg accggcgctc gcacaggcac ccgccgcgcc gcacaacatg gccaacatgc
     1741 cgccgcccgc gtcgggcgcg aaggtcatcg tgagcgaccc gactcaggtc gtcatgatcg
     1801 tggaccgcga ctcgaacgac atcgcgttca tggacatcaa gaccaagcag atcctgggcc
     1861 gcacgttctt aggcaacaac gtgaacccgc acatggtcat gatgtcgccc gacggccgct
     1921 acgtcgtcac cggcggccag cgctcgaaca cggcgtacat catcgacagc cgcacgctcg
     1981 agctcgtggc gaagatcccc gttgggttcg cgcccgagca cctcgcgttc tcgcccgacg
     2041 gccgttggta ctaccagggc aatcccgaag gcgactcgat caccgtcatc gacatgcagt
     2101 cgctcaaggc ggtgaagacg atcacgggtc tgcacgagcc gctcaacatc acgttcacgc
     2161 ccgacggcgc caaggcgtac atcggcaact acggcgcgca ctgggtcggc gtcgtcgacg
     2221 tcacccggca cgagctcatc aagaggatca acatcgatac cgttccgggc gtcgcgcgcc
     2281 tcgatccggc gaagtacctc accgagatca agggcatctt caacgccact cccacacccg
     2341 acggccgctt cctctacgcc gccgacggcg atctcggcgt ggtcggcgtg atcgacacgc
     2401 gcgaggacaa ggtcatcaag gtgattcgtg tcgggccgga tccgtggcgc gtgtacatga
     2461 gccacgacgg caagtacggc atctcggtga acaacggcga cagcacggtc tcgatcatcg
     2521 acatcgcgaa gaacgccgtc gtcgccacgt acccggtagg cccggacctc acgggcatca
     2581 actttgccgc aggcaaagcg ttcgtgatca gctccacgac gggcttcctc tatgtgttcg
     2641 acatggccac gctgaagccc gcgggccgga tcaagatcgg caacaacttg ttcttgcaga
     2701 cggcgacgac cgacacggcc gatcaaaagc tgtatctcgg cagcgcgacg gacaactcgg
     2761 tttacatcat cgacgctgca accgaggccg tggagcgcgt agcgaacgtg
//
LOCUS       DASUGA010000684         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_103138, whole genome shotgun sequence.
ACCESSION   DASUGA010000684 DASUGA010000000
VERSION     DASUGA010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103138"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1426
                     /locus_tag="VFX21_13630"
     CDS             71..1426
                     /locus_tag="VFX21_13630"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX5097060.1"
                     /translation="MSVRITVVGTGHVGSVVGGCLAMIGHQVVGLEVDRAKLEHLQRG
                     TAPFFEPGADELLRDASATGRLRFTDDFDDAVHASDVVFLCVGTPTTANGHADTTALE
                     AAARAVAEAMDGPKVLVSKSTVPIGSGHWLETIVEDALPPDRRDIRFAVASNPEFLRQ
                     GSSIADYLYPDRVVLGSDDVRALSVLQEIYRPILDQSFPGGVPARRPALVCTNLATAE
                     TIKYASNAFLALKVSFANEIANLCEHVGADSLDVARALGLDRRIGPDFLNAGAGWGGS
                     CFGKDLGELIAVAGDHGYNAPLLRAARDVNIIQRGLVVRKLRQSLHGLRGRRIGLLGL
                     AFKPGTDDVRDAPALDVARALVDAGARVIAHDPVVGTLPDVPCVRVVDDPYGVAERAD
                     AVVLMTEWPEYRSLELDVLASRMRGMLFVDGRNVFDPEKAEIAGLLYEGIGRSRSARA
                     R"
     gene            1469..>2033
                     /locus_tag="VFX21_13635"
     CDS             1469..>2033
                     /locus_tag="VFX21_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097061.1"
                     /translation="MSRVAATVMSPAWSQPFDLWFDVPTDRADMAATCPAADPWVAAL
                     LLTAMRHGEDLRVDAPLTEDLRRALPQVQNVFAAWMPSATTIAVDAAGTYTSGVRGDG
                     VGLCFSCGVDSWYSLLRSEDRRRFGRPPDVTHLLHVHGVDIDVGRWKADVAGTVAAHT
                     IRIADAFGLTPVTIATNVRQLYSRVGLS"
BASE COUNT          276 a          702 c          709 g          346 t
ORIGIN      
        1 gaggtctcgc gaaggcggcg cgcgcgatgg cggagctcga ctcgtcgtcg ctcgtcgcgg
       61 agggtcggcg gtgagcgtgc ggatcaccgt tgtcgggacc ggtcacgtcg ggtcggtcgt
      121 cggcgggtgc ttggcgatga tcggccatca ggtcgtcggg ctcgaagtcg accgcgccaa
      181 gctcgagcac ctgcagcgcg ggacggcgcc gttcttcgaa ccgggtgcgg atgagctcct
      241 tcgcgatgcg tccgcgaccg gacggttgcg gttcaccgac gatttcgatg acgccgtcca
      301 cgcgagcgat gtcgtgttct tgtgcgtggg cacaccgacg accgcgaacg gccacgccga
      361 caccaccgcg ctcgaggccg ccgctcgcgc cgtcgccgaa gcgatggacg gcccgaaggt
      421 cctggtgagc aagagcaccg tccccatcgg gagcggccac tggctcgaga cgatcgtcga
      481 ggacgccttg ccgcccgatc ggcgggacat ccggttcgcc gtcgcgtcga acccggagtt
      541 cctgcgccag ggctcgtcga tcgccgacta tctctatccc gaccgtgtcg tcctgggcag
      601 tgacgatgtc cgcgcgctct cggtcctcca ggagatctac cggccgattc tcgaccagtc
      661 attccctggc ggtgtgcccg cccgacgtcc tgcactcgtg tgtacgaacc ttgcgacggc
      721 agaaacgatc aagtacgcgt cgaacgcctt cctcgcgttg aaggtcagct tcgcgaacga
      781 gattgcgaac ctgtgtgagc acgttggcgc ggactcgctc gacgtggcgc gcgcgttggg
      841 gttggaccgt cgaatcgggc cggacttcct caacgcgggc gcgggatggg gcggttcgtg
      901 cttcggcaag gatctcggcg agctgatcgc cgtcgcgggc gaccacggct acaacgcacc
      961 gttgttgcgc gcggcgcgtg acgtgaacat catccaacgc gggctcgtcg tgcgaaagct
     1021 gcggcagtcc ctccacggtc tccgcggtcg gcggatcggc ctcctcggcc tcgcgttcaa
     1081 gccgggcacc gacgacgtgc gggatgcgcc tgcgctcgac gttgcgcgcg cgctcgtcga
     1141 cgccggcgcg cgcgtcatcg cgcacgaccc cgtcgtcggc accctcccgg acgtgccgtg
     1201 cgtgcgcgtc gtcgacgacc cctacggcgt ggccgagcgc gccgacgccg tggtcctcat
     1261 gacggagtgg cccgagtacc ggagcctcga gctcgacgtg ctcgcgagcc gcatgcgggg
     1321 aatgctgttc gtcgacggcc gcaacgtgtt cgaccccgag aaggccgaga tcgccggact
     1381 gctctacgag ggcatcggac gttctcggtc ggcaagggcg cggtgatccg tgcacgtcgg
     1441 cgaccttcgt gtcgatcgtc gcggcgacgt gagtcgcgtc gctgccaccg tgatgtcgcc
     1501 tgcgtggtct caacccttcg acctgtggtt cgacgtccca accgatcggg cggacatggc
     1561 ggcgacgtgc cccgccgcgg atccgtgggt cgctgcgttg ctgctgaccg ccatgcggca
     1621 cggcgaggac ctgcgcgtcg atgcgccact caccgaggat ctgcgtcgcg ctctcccaca
     1681 agtccagaac gtgttcgcgg cctggatgcc cagcgcgacg acgatcgccg tcgacgccgc
     1741 gggtacgtac acgagcggcg tgcgcgggga tggcgtcggc ctgtgcttct cgtgcggcgt
     1801 ggactcctgg tattcgttgc tccgctccga ggaccgccgc cgcttcggcc gccctccgga
     1861 cgtcacgcac ctgctccacg tccacggtgt cgacatcgat gtcggtcgct ggaaggccga
     1921 cgtggcggga acggtggcgg cgcacacgat ccggatcgcc gacgccttcg gactcacgcc
     1981 cgtgacgatc gcgaccaacg tgcgccagct ctactcgcgc gtcggcctct cgt
//
LOCUS       DASUGA010000685         5288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1143939, whole genome shotgun sequence.
ACCESSION   DASUGA010000685 DASUGA010000000
VERSION     DASUGA010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143939"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2116
                     /locus_tag="VFX21_13640"
     CDS             <1..2116
                     /locus_tag="VFX21_13640"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5097062.1"
                     /translation="LVGGADGDLLTSVAGKAGLAAQMKEEPGSAAAQAREADNLATLA
                     RYQAIPAGQRTFDNPEWVKFLLYDNQGNVRKALRSFFLDETHAQIITRLKGNESIEDE
                     GAAATFVKDEAEKLELPNTSTVTTGASILLKDINDYLKSGMLTLGAIAVAIMIVILLL
                     LFNVRWRLLPMLVILFGVIWAFGVAGYVGIPLTVVTIAGLPVMLGIGIDYAIQMHARV
                     EEEAIIDRADHPIQETARSLGPALLVVTFDAVFAFAALRFAKVPMLRQFGLLLAVGIM
                     VICTASIIIPLAILGMREYRSPTKGRDFSEGFLGRFVVKLGSGRSSFAIPLAVASLLI
                     FAGGIIVEDKLTLQTDPIQWVNQSSQTIKDIHTVEREAGGSSEIGIYATATTGDVFSD
                     EFVDFEHTFTQEQLDKFPGKLLTGSSIETSIGDLLEVPGASDIPPTGQDIRDAYDVAP
                     PDLQNSAVSPDGKAVNIVLRTGPGSLEERAPVVREIRETTQPPEGITATPSGLAVVGV
                     GLLDNLEANRILLTYLAILFVFLFLAVRLRSIIRALLSLVPVLIAVGLSSLVAWLAGW
                     KLSPMTAVGGPLVVAACTEFTSLILLRFVEERGRGLEPREAVDVAAARTGRAFIVSAL
                     TAIAGVAVLSFSSLPLLRDFGRIVAMNVAVALLSALVVLPPLLVWADKRNWVSRGLIK
                     KPEPFIPTPPHDVVPTPGIATP"
     gene            2230..3306
                     /locus_tag="VFX21_13645"
     CDS             2230..3306
                     /locus_tag="VFX21_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097063.1"
                     /translation="MADLVIHAGMPKAGTSSVQWWLRTHLDEVAGNGFEVRRRPRDLD
                     SATCLDEMTCAATPRGVKSYVDDLLGDAHDQRLLLDALHALPGTVLLTSEAFNNLFNR
                     DATWLPALNDYAAVHDVRVAYYVRPQHRAMEASWRQWGFRTRVRPSVFLEKREGRRLH
                     YVSTLFTVRSGAPNVSFEVRGLRRDLLVGGDIVVDFAKTFLDMQAEPVPPENEGLPLF
                     VCVLLQTRGACPLWTSQRDNRALTRLKRAVARWNISEDDAIRESRLVLQRHCRSRYER
                     GNRKLGSLLKWPSEDFVPPTYEAGDDRLSRLDVLWRPRPEGDLLRQACADIIATANTR
                     DARRSRPASVRVTQRQPLTLSARR"
     gene            complement(3290..4165)
                     /locus_tag="VFX21_13650"
     CDS             complement(3290..4165)
                     /locus_tag="VFX21_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5097064.1"
                     /translation="MICAGRVAVVTGAGRGIGRGHALELARQGAKVVVNDVGASVDGT
                     GADTGPAQQVVDEITAAGGEAIANTDDVADWEGARRLVYTAIDTFGTIDVLVNNAGIL
                     RDRMLINMEEAEWDAVIRVHLKGTFAPSRHAAAYWREKSKAGEPVDARIINTSSTSGL
                     YANPGQTNYGAAKSGIATFSIIAAKELSRYGVTVNAIAPGARTRMTENLGGGMAAPSE
                     GEFDARDPENIAPLIAWLASPESKDVTGRVFLVGGGRIGVATPWGRGEMVDRGARWDP
                     ADLGAVIPKLLTDER"
     gene            complement(4239..>5288)
                     /locus_tag="VFX21_13655"
     CDS             complement(4239..>5288)
                     /locus_tag="VFX21_13655"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5097065.1"
                     /translation="DPALKAWTQRLHDAGFLCVSWPKEFGGRGLTGVEVAVLNEEFAN
                     AKVPRVTRGMGEWLVGPSIIVHGTDEQKAKFLPRIIDGTDRYCQGFSEPDAGSDLAGL
                     KTRGVIDGDEVVITGQKVWTSGAHLANMMFCLCRTDPDAPKHRGISYVLVPMKRDNGA
                     SNGFELRPIKQITGTSGFTETFITEARAPVANIIGGLNDGWRVTMTTLGNERGGNATT
                     QHVQYQKQFWRIVKEVRRLDKQDDPLVRQKLAWAFTRAEIMRYAGLRTLSEVVARKEP
                     GPGASINKMFWSEYARDVSEWVMNMRGAQSMLGPDRWGADFLGSRSGTIWGGTAQVQR
                     NIVGERVLGLPKEPE"
BASE COUNT          815 a         1782 c         1756 g          935 t
ORIGIN      
        1 gctcgtcggc ggtgcggacg gcgatctcct gacgagcgtc gcgggcaagg cgggcctggc
       61 cgcacagatg aaagaagagc cgggctcagc ggccgcccag gcccgtgagg cagacaacct
      121 ggcgacgctg gcgcggtacc aggcgatccc cgccgggcag cgcaccttcg acaacccgga
      181 gtgggtgaag ttcctcctct acgacaacca gggcaacgtc cggaaggcgt tgcgatcgtt
      241 cttcctcgac gagacgcacg cgcagatcat cacgcgtctg aagggcaacg aatcgatcga
      301 agacgaaggc gcggccgcaa cgttcgtgaa ggacgaagcg gagaagctcg agctgcccaa
      361 tacgtcgacg gtcaccacgg gcgcgtcgat tctcctcaag gacatcaacg actatctcaa
      421 gagcggcatg ctgacgctcg gcgcgatcgc ggtcgcgatc atgatcgtga tcctgctcct
      481 gctgttcaac gtgcgttggc ggctgttgcc gatgctggtc atcttgttcg gcgtcatctg
      541 ggcgttcggt gtcgccggct atgtcgggat ccccctcacc gtcgtgacca tcgcgggcct
      601 gccggtcatg ctcggcatcg ggatcgacta cgcgatccag atgcacgcac gcgtcgagga
      661 agaggcgatc atcgaccgcg ccgatcaccc catccaggaa acggcccgca gtctcgggcc
      721 cgcgctgttg gtcgtgacgt tcgacgcggt cttcgcgttc gcggcactgc gattcgcgaa
      781 ggtgccgatg ctgcgccagt tcgggctcct gctcgcggtc ggcatcatgg tgatctgcac
      841 cgcgtcgatc atcatcccgc tcgcgatcct cgggatgcgc gagtaccgct cgccgaccaa
      901 gggccgcgac ttcagcgagg ggttcctcgg ccgtttcgtc gtgaagctcg gctcgggccg
      961 gtcgtcgttc gcgatcccgc tggcggttgc gagcttgctc atcttcgcgg gcggcatcat
     1021 cgtcgaggac aagctcacgc tgcagaccga cccgatccag tgggtgaacc agagctcgca
     1081 gacgatcaag gacatccaca ccgtcgagcg cgaagcgggt gggtcgagcg agatcgggat
     1141 ctacgcgacg gcgacgaccg gtgacgtgtt ctccgacgag ttcgtcgact tcgagcacac
     1201 gttcacgcaa gagcagctcg acaagttccc gggcaagctg ttgacgggtt ccagcatcga
     1261 gacgtcgatc ggcgacctgc tcgaggtacc gggcgcgtcc gacatcccgc cgacaggcca
     1321 agacatccgc gacgcgtacg acgtcgcacc gccggacctg cagaactccg ccgtgagccc
     1381 cgacggcaag gccgtcaaca tcgtgttgcg gaccggaccg ggatcgttgg aggagcgggc
     1441 gcccgtcgta cgcgagatcc gcgagacgac gcagccgccc gaaggcatca ctgccacacc
     1501 gtcgggcctc gcggtcgtcg gcgtcggtct gctcgacaac ctcgaagcga accggatcct
     1561 gttgacctat ctcgccatct tgttcgtgtt cttgttcctc gcagtgcggc tgcggtcgat
     1621 catccgggcg ctgctgtcgt tggtgccggt gttgatcgcg gtcggcctgt cgtcgttggt
     1681 ggcgtggctc gccgggtgga agttgagccc gatgactgcg gtgggtggac cattggtcgt
     1741 cgccgcgtgt accgagttca cgtcgttgat cctgttgcgg ttcgtcgaag agcgcggccg
     1801 cggcctcgag ccgcgagaag cagtcgacgt cgcggcggcg cggaccgggc gtgccttcat
     1861 cgtgtcggcg ttgaccgcga tcgcgggcgt cgcagtcttg tcgttctcgt cgttgccgtt
     1921 gctgcgcgac ttcgggcgca tcgtcgcgat gaacgtcgcg gtcgcgttgc tgagcgcgct
     1981 ggtcgtgttg ccgccgctgc tggtgtgggc cgacaagcgc aactgggtgt cgcggggcct
     2041 catcaagaag cccgagccct tcatccccac gccgccccac gacgtcgtcc cgacaccggg
     2101 catcgccacg ccctgaacac ttgccggcga ggccctgacg ccgcctccgg accgcgacgg
     2161 tcaggcggat attgcggcgc gccaaccggc ttcgtacgct ttccgtgcgg ggtgtccccg
     2221 ggggggcaca tggctgatct ggtcatccac gccggcatgc cgaaggcggg cacgtcgtcg
     2281 gtgcagtggt ggctgaggac gcacctcgac gaggtcgccg gcaacggatt cgaggttcga
     2341 cgccggccgc gtgatctcga cagcgcgaca tgtctcgacg agatgacatg tgctgcaaca
     2401 ccgcgcggag tgaaaagcta cgtcgacgat ctcctcggcg acgcacacga tcagcggcta
     2461 ctgcttgacg cactccacgc gctacccggc accgtgctct tgacgagcga agcgttcaac
     2521 aacctcttca accgggacgc cacctggtta cccgccttga acgactatgc cgcggtgcac
     2581 gacgtccgcg ttgcgtacta cgtgcgtccg cagcaccgcg cgatggaagc ctcgtggcgg
     2641 caatggggct tccgaacacg ggtgaggccg tcggtatttc tcgaaaagcg tgaaggccgc
     2701 cgactccact atgtctccac gctcttcact gtgcgcagcg gtgcgcccaa cgtgtccttc
     2761 gaagtgcgcg ggcttcgtcg cgatctcctc gttggcggag acatcgttgt cgactttgcg
     2821 aagactttcc tcgatatgca ggccgagccc gtcccgcccg agaacgaagg acttccgctt
     2881 ttcgtatgcg tcttgctcca gacgcgcggc gcgtgcccgc tctggacaag tcagcgcgac
     2941 aaccgggcgc tgacgcgtct gaagagggcc gtcgcacgtt ggaacatttc cgaagacgac
     3001 gcgatccgtg aatcacggct cgtattgcaa cgccactgcc gatcacgata cgagagaggc
     3061 aatcgcaagt tgggctcgct gctcaaatgg ccgtcggaag acttcgtccc tccgacatac
     3121 gaagcaggcg acgatcggct gtcgcgcctc gacgtgctct ggcggccgcg gccggaaggc
     3181 gacctgctgc ggcaagcgtg cgccgacatc atcgccaccg caaacacgcg agatgcacga
     3241 cggtcccgac ctgcatccgt gcgcgtgacc cagcgacagc ccttgaccct tagcgctcgt
     3301 cggtgagcag cttcgggatg actgcgccga ggtcggcggg gtcccagcgg gcaccgcggt
     3361 cgaccatctc gccgcggccc cagggtgttg cgacgccgat gcggccgccg ccgacgagga
     3421 acacgcggcc ggtgacgtcc ttcgactcgg gcgaggccag ccacgcgatg agcggcgcga
     3481 tgttctcggg gtcgcgcgcg tcgaactcgc cctcggaggg cgcggccatg ccgccaccga
     3541 ggttctccgt catacgcgtc cgcgcgccgg gcgcgatcgc gttgacggtc acgccgtagc
     3601 ggctgagctc cttggcggcg atgatcgaga acgtcgcgat gcccgacttc gcggcgccgt
     3661 agttcgtctg ccccgggttc gcgtacaagc ccgatgtcga cgacgtgttg atgatccgcg
     3721 cgtcgaccgg ctcccccgcc ttcgacttct cccgccagta cgcagccgca tgccgtgacg
     3781 gcgcgaacgt gcccttgagg tggacgcgaa tcaccgcgtc ccactcggct tcctccatgt
     3841 tgatgagcat ccggtcgcgg aggatgccgg cgttgttgac gaggacgtcg atggtgccga
     3901 acgtgtcgat cgccgtgtac acgaggcggc gcgcgccctc ccagtcggcg acgtcgtcgg
     3961 tgttcgcgat cgcctcgccg ccggccgccg tgatctcgtc gacgacttgt tgcgcgggac
     4021 cggtatcggc gccggtgccg tcgaccgacg cgccgacgtc gttgacgacg accttggcac
     4081 cctggcgggc cagttcgagc gcgtggcccc ggccgatgcc gcggccggcg ccggtgacga
     4141 cggcgacgcg cccggcgcag atcattccgg ctccttgggt agtccgagca cacccgcccc
     4201 ccgaccccgc ccaccgcccc gtaaggagtg ctcgacactc attccggctc cttgggtagt
     4261 ccgagcacac gttctccgac gatgttgcgc tgcacttgcg cagtgccgcc ccagatggtg
     4321 cccgagcgcg agccgaggaa gtcggcaccc cagcgatcgg ggccgagcat cgactgcgcg
     4381 ccgcgcatgt tcatcaccca ttcgctgacg tcacgcgcgt actccgacca gaacatcttg
     4441 ttgatcgacg ccccggggcc cggctccttg cgggcgacga cttcggagag cgtgcgcagc
     4501 ccggcgtagc gcatgatctc cgcgcgtgtg aatgcccacg cgagcttctg gcgcacgagc
     4561 gggtcgtctt gcttgtcgag gcggcggact tccttgacga tgcgccagaa ctgcttctgg
     4621 tattgcacgt gttgcgtcgt ggcgttgccg ccgcgttcgt tgccgagcgt cgtcatggtg
     4681 acgcgccagc cgtcgttgag accgccgatg atgttcgcga cgggtgcgcg cgcttcggtg
     4741 atgaacgtct cggtgaagcc gctcgtcccg gtgatctgct tgatcggccg cagctcgaag
     4801 ccgttcgatg ctccgttgtc ccgcttcatc ggcacgagca cgtaggagat gccgcggtgc
     4861 ttgggcgcgt ccggatcggt gcggcacagg cagaacatca tgttggcgag gtgcgcgccc
     4921 gaggtccaca ccttctgccc cgtgatcacg acctcgtcgc cgtcgatcac gccacgcgtc
     4981 ttcaggccgg caaggtcgga gccggcatcg ggttcggaga agccctggca gtaacggtcg
     5041 gtgccgtcga tgatgcgcgg caggaacttc gccttctgtt cgtcggtgcc gtgcacgatg
     5101 atcgacggac cgacgagcca ctcccccatg cctcgcgtga cgcgtgggac cttcgcgttc
     5161 gcgaactcct cgttcagcac cgcgacttcc acgccggtca gcccgcgccc gccgaactcc
     5221 ttgggccagc tgacgcagag gaagcccgcg tcgtgcagcc gctgcgtcca cgccttgagc
     5281 gcggggtc
//
LOCUS       DASUGA010000686         2650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_727561, whole genome shotgun sequence.
ACCESSION   DASUGA010000686 DASUGA010000000
VERSION     DASUGA010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727561"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VFX21_13660"
     CDS             <1..423
                     /locus_tag="VFX21_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097066.1"
                     /translation="FSSCTGRPVPVIAFHGVKDPIVGYDGGGLNATQIADNQLWKGSK
                     PAGLPQHRGVDAALQTWAEHNWCDPQPVTKRISAEVQHRTWRHCDADTELYIVDNGGH
                     SWPGRPVPGFADQFGHTTTDVDATQLIFTFFWAHPSCA"
     gene            complement(426..1670)
                     /locus_tag="VFX21_13665"
     CDS             complement(426..1670)
                     /locus_tag="VFX21_13665"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5097067.1"
                     /translation="MRVSAAIRRTFGSLDTYNFRLYFSGDLVSFVGSWMQNMAEAWLV
                     FELTGSGAAVGATFAFRFLPVAVFGLWGGAIADHFDRRKVLLITSTLSAVLAVVLWFV
                     VLTGAVSVWMVFALATALGFVTVVDEPARQAFMQEMVGRDRLANAVALNSAVHNSARI
                     TGPAIAGILIATAGVSWVFFANALTFFAFIAALLAMRVRELQPPQRHEERPRVREGLV
                     YAWSLTDIRVTIVLVAVVGTLVYNFPTYLTLMASETFGGGAGLAGFLMAMLGIGMVVG
                     GLVAAHRGHSTRRIVIIAALLLGSAMFVTALLPTLLLVEIALIPVGVLAVFFGTSANA
                     HMQLASAHELRGRVMAIYMLLTLGTTVVGGPFVGWVCQQVSPRFGFGMAGVATATAAV
                     LAALHTTRARRAAPQYAIADAA"
     gene            1738..2322
                     /locus_tag="VFX21_13670"
     CDS             1738..2322
                     /locus_tag="VFX21_13670"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX5097068.1"
                     /translation="MRRTQAERSATTQQALTKAAIDTLVERGWAATTAVEVCERAGVT
                     RGALMHHYPSLAALLAAALQTLYDDVAATAPRVRSLRALLDFTWDRLGRREMKAMFEA
                     WLAAANNRDLAAEIGPVVASFSKLVDPHELPDVQFLDTPDAISFYLVAREAMLGLAMG
                     RATTGGAGVKHEKQVLARLRAEASAFDAQRKAAS"
     gene            2319..>2650
                     /locus_tag="VFX21_13675"
     CDS             2319..>2650
                     /locus_tag="VFX21_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5097069.1"
                     /translation="MTTVTKHVVCTICDIGCQLRAVADDGKVTKILPHEHPLLAKNIC
                     YKGTAAPQIHNHADRLRTPLKRAGRRGENRWEPISYAQAMDEIAERLQTIVDTHGPEA
                     LAVSTSNW"
BASE COUNT          501 a          888 c          891 g          370 t
ORIGIN      
        1 ttctcgtcgt gcactggccg gcccgtgcct gtgatcgcgt tccacggcgt caaggatccg
       61 atcgtcggtt acgacggcgg tgggctcaac gcgacgcaga tcgccgacaa ccagctttgg
      121 aagggatcga agccggccgg cctgccgcag catcgcggtg ttgacgccgc gttgcagact
      181 tgggccgagc acaactggtg cgatccgcaa ccggttacga agcgcatcag cgccgaggtg
      241 cagcaccgca cgtggcgaca ctgcgacgcc gacaccgagt tgtacatcgt cgacaacggc
      301 gggcactcgt ggcccggtcg tcccgtgccg ggcttcgccg atcagttcgg ccacaccacg
      361 accgacgtcg acgcgacgca gctcatcttc accttcttct gggcccatcc gtcctgcgcg
      421 tagaactagg ccgcgtcggc gatggcgtat tgcggagcgg ctcggcgggc acgcgtcgtg
      481 tgcaacgcgg cgagcactgc cgcggtcgcg gttgcgactc cagccatgcc gaagccaaac
      541 cggggactga cctgctggca cacccagccg acgaacgggc cgccgacgac ggtggttccc
      601 agcgtcaaca gcatgtagat cgccatgacg cgcccccgta gctcgtgggc cgaagcgagc
      661 tgcatgtgcg cgttggccga cgtcccgaag aacaccgcga gtacaccgac cgggatgagt
      721 gcgatctcga cgagcagcag cgtggggagc agcgcggtga cgaacatcgc gctaccgagg
      781 aggagcgccg cgatgatcac gatgcggcgg gtcgaatggc cgcggtgcgc ggcgaccaac
      841 ccgccgacga ccatgccgat accgagcatc gccatgagga accccgcgag ccctgcaccg
      901 ccgccgaacg tctcgctcgc catcagcgtg aggtacgtcg ggaagttgta gacgagggtg
      961 ccgacgaccg cgacgagcac gatcgtgacg cgtatgtcgg tcaggctcca cgcgtacacg
     1021 aggccctcgc gcactcgtgg acgctcctcg tggcgctgcg gcggctgaag ctctcgcacg
     1081 cgcatggcga gaagggcagc aatgaacgcg aagaaggtca gcgcgtttgc gaagaacacc
     1141 cacgacacgc cggcggtggc gatcagaatg ccggcgatcg ccggtccggt gatgcgcgcc
     1201 gagttgtgga ccgcgctgtt caacgccact gcattcgcga gccggtcgcg gcccaccatc
     1261 tcctgcatga acgcctggcg ggccggctcg tcgaccaccg tcacgaagcc gagcgcggtc
     1321 gcgagcgcga acaccatcca caccgagacg gcacccgtga gcacgacaaa ccaaagcacg
     1381 acggcgagga ccgctgacag cgtcgacgta atgagcagca ccttgcgccg gtcgaagtgg
     1441 tcggcgatcg cgccgcccca gagtccgaag acggcgaccg gcaagaagcg gaacgcgaac
     1501 gtcgcgccga ccgcggcacc gctcccggtc agctcgaaca cgagccacgc ctcggccatg
     1561 ttctgcatcc aggaaccgac gaacgagacg agatcgccgg agaagtagag gcggaagtta
     1621 taggtgtcca gggagccgaa cgttcgtcgt atggctgccg aaacccgcat cagccgtgaa
     1681 tttaccggac acttacatac agttgacaat gtatgtaagt gcgcggagaa tgccggggtg
     1741 cgacggaccc aggcggagcg ctccgcgacc acgcagcagg ccctgaccaa ggccgcgatc
     1801 gacacgctgg tcgagcgggg gtgggcggcg acgaccgcgg tcgaagtctg cgagcgcgcg
     1861 ggcgtgacgc ggggcgcgct catgcaccac tacccgagcc tcgcggcgct cctcgccgcg
     1921 gcattgcaga ccctctatga cgacgtcgcg gctaccgcgc cgcgcgtgcg ttctctgcgc
     1981 gccctgctcg acttcacctg ggaccggctc gggcggcgcg agatgaaggc gatgttcgaa
     2041 gcgtggctcg cggccgcgaa caaccgtgac ctcgcagccg agatcggacc ggtcgtcgcg
     2101 tcattctcga agctcgtcga cccgcacgag ctgcccgacg tgcagttcct cgacacaccg
     2161 gacgcgatct cgttttatct cgttgcccgt gaagcgatgc tcggactcgc gatgggccgg
     2221 gcgaccaccg gcggcgcggg cgtcaagcac gagaagcagg tgctggcgcg cttgcgagcg
     2281 gaagcgtcgg cgttcgacgc gcagcgaaag gcagcctcat gacgaccgtg accaaacacg
     2341 tcgtgtgcac gatctgcgat atcggctgcc agctgcgcgc ggtggcggac gacggcaagg
     2401 tgacgaaaat ccttccccac gagcatccgc tgctcgcgaa gaacatctgt tacaagggca
     2461 ccgccgcgcc ccagatccac aatcacgccg atcgcttgcg gacgccgctg aagcgcgccg
     2521 gtcgccgggg tgagaaccgg tgggagccga tttcgtacgc acaggcgatg gacgagatcg
     2581 ccgaacggtt gcagacgatc gtcgacaccc acggtcccga ggcgctggcc gtctcgacct
     2641 cgaactggaa
//
LOCUS       DASUGA010000687         3429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1562166, whole genome shotgun sequence.
ACCESSION   DASUGA010000687 DASUGA010000000
VERSION     DASUGA010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562166"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1465
                     /locus_tag="VFX21_13680"
     CDS             158..1465
                     /locus_tag="VFX21_13680"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5097070.1"
                     /translation="MAKRVWCGALLALVALTAFPAAGAGAQEAPPTTAGAGAAIQGVM
                     QSGSTPIEGVRVLVTGVDDPSFEADATSDQEGHWIIPVPGRGVYRVAIDPSTIPEEFE
                     LANPDRIELPNFRVFADRPQNVVFAFKSDEGGIAVPSRWERLLNLFVSGVRLGLIIGV
                     CSVGLSLVFGTTGLVNFAHGELVTFGALVAFYFNTSSSGPQWSLVIATLLAMVGGALL
                     GSALELGLWRPMVRRRSGVMARMLVSIGLALALRYTYEFIFGAGPRAFREFSAQGPLD
                     FGPLTMPARDYWIMAISVCVLVGVGMILQLSRLGTAVRAVADERDLAAASGIDVKRVI
                     LYVWAGGTALAALGGVMLAVSQSVQYNLGFRLLLTMFAAVVLGGLGSAYGAMVGGLAV
                     GIAQEVSTYWISPDYKFAVALVILILVLLVRPQGILGVRERVG"
     gene            1467..2462
                     /locus_tag="VFX21_13685"
     CDS             1467..2462
                     /locus_tag="VFX21_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007024637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5097071.1"
                     /translation="MDWSSIFENGMRDAVGIVAAVYALTAVGINLQFGYTGLANYGQA
                     GFLLVGAYGTAIAVDQWDLSLWVAFLVGLLAAVGLGLLLGIPTLRLRADYLAIVTISA
                     AEALRIVVNSRAAQDLTGGPQGIHGFADAFFDVNPFDGRFKIIGAWAYSNRDLWVTVI
                     AWGAVALASFALYLLTRSPWGRVLRAVREDEDAARSLGKNVYAFKMQSLLIGGAIGGV
                     AGMLLAVDRQFVDAQQFVANVTFLAYAALILGGLGRILGPVVGSMVLWFFVSALNQFL
                     REAVANDFLGITHVMDENDVGAVRFILVGVLIMVLVIFRPQGILGSRSEVMLGDG"
     gene            2488..3249
                     /locus_tag="VFX21_13690"
     CDS             2488..3249
                     /locus_tag="VFX21_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5097072.1"
                     /translation="MLRVSGLRRTFGGLTAVDVDELQIKRNTITALIGPNGAGKTTMF
                     NLLTGFDKPDGGAWWFDGQDMSGLSAYRVARLGMVRTFQLTKSLARLTVLENMLLGAP
                     GQQGERLLPALFHTWRGQERANTGRARALLERFKLADKQRDLAGTLSGGQRKLLEMAR
                     ALMVDPKLIMLDEPMAGVNPALVQSLLEHIKELRAEGRTVVFVEHDMDVVMNISDWVV
                     CMAEGHVIAEDVPARIIANEAVIEAYLGLRHGDAS"
     gene            3246..>3429
                     /locus_tag="VFX21_13695"
     CDS             3246..>3429
                     /locus_tag="VFX21_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019808113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5097073.1"
                     /translation="MNLLEARDVVAGYLPGIDILHGCNLDVGEGELVGIIGPNGAGKS
                     TLIKAIFGLVTVRSGTI"
BASE COUNT          483 a         1146 c         1194 g          606 t
ORIGIN      
        1 cgtgccggac gtggtccctc cggcggcagg cggcggcatg agaaccaagg cagtcgaagg
       61 atcggggagg tgcgagcagc gcctccccga tccgcgttcc ggggaccggc ggttgctcgt
      121 atgctgcgcc gcaaattcac cgctccgggg ggactgggtg gccaagaggg tgtggtgcgg
      181 tgcgctgctg gctctcgtcg ccctcaccgc gttccccgct gccggggcgg gagcgcaaga
      241 agcaccgccc acgaccgccg gcgccggcgc ggccattcag ggcgtcatgc agagcgggtc
      301 gacaccgatc gaaggcgtcc gggtgctcgt caccggcgtc gacgatccga gcttcgaagc
      361 cgacgcgacg tccgaccaag agggccattg gatcatcccg gtgcccgggc gcggcgtcta
      421 ccgcgtggcg atcgacccct cgacgatccc cgaagagttc gagctcgcga acccggatcg
      481 catcgagttg cccaacttca gggtgttcgc cgaccggccg cagaacgtcg tcttcgcatt
      541 caagagcgac gagggcggca tcgcggtacc gagccgctgg gagcggttgc tgaacctgtt
      601 cgtcagtggc gtccggctgg gcctgatcat cggtgtgtgc tcggtcggcc tttcgctcgt
      661 cttcggcacg accggtctcg tgaacttcgc gcacggcgag ctcgtcacgt tcggcgcgct
      721 cgtcgcgttc tacttcaaca cctcgtcgtc ggggccgcag tggtcactcg tgatcgcgac
      781 cttgctcgcg atggtcgggg gcgcgttgct cggcagcgcg ctcgagctcg ggctgtggcg
      841 accgatggtg cggcgccgca gcggcgtaat ggcccgcatg ctcgtgtcga tcgggctcgc
      901 gctcgcgctg cgctacacgt acgagttcat cttcggtgcc ggtccgcgcg cgttccgcga
      961 gttctccgcg caagggccgc tcgacttcgg cccgctcacg atgccggcgc gcgactactg
     1021 gatcatggcg atcagcgtct gtgtgctcgt cggtgtgggg atgatcctcc agttgtcacg
     1081 gctcggcacg gctgtgcgcg ccgtggccga cgagcgggac ctcgccgccg cctcgggcat
     1141 cgacgtgaag cgcgtcatct tgtacgtgtg ggcaggaggg accgcgctcg ccgcgctggg
     1201 cggtgtgatg ctcgcggtgt cgcagagcgt gcagtacaac ctggggttcc ggctgctcct
     1261 cacgatgttc gccgccgtgg tgctcggcgg cctcgggagc gcgtacggcg cgatggtcgg
     1321 cggcctcgcc gtgggtatcg cccaagaggt gagcacgtac tggatctcgc ccgactacaa
     1381 gttcgcggtc gcgctcgtca tcctcatcct cgtgctcctg gtacgaccgc agggcattct
     1441 cggcgtgcgc gagcgggtgg gctgacatgg actggtcgag catcttcgag aacggcatgc
     1501 gcgacgcggt cggcatcgtc gccgcggtgt acgcgctcac cgcggtcggt atcaacctgc
     1561 agttcggcta caccgggctc gccaactacg gacaggccgg cttcctgctc gtcggcgcgt
     1621 acggcaccgc gatcgcggtc gaccagtggg acctgtcgtt gtgggtcgcg ttcctcgtcg
     1681 ggctcctcgc cgcggtcggg ctcggcctgt tgctcggcat cccgacgttg cgcctgcgcg
     1741 cggactattt ggcgatcgtc acgatctccg ccgcggaggc cttacgcatc gtcgtgaact
     1801 cgcgcgcggc ccaggacctc accggcgggc cgcagggcat tcacggattt gccgatgcct
     1861 tcttcgacgt caatccgttc gacgggaggt tcaagatcat cggtgcgtgg gcgtacagca
     1921 accgcgactt gtgggtcacg gtgatcgcgt ggggtgcggt cgcgctcgcg agcttcgcgc
     1981 tgtacctgct gacccgcagc ccgtggggcc gcgtcctgcg cgccgttcgc gaagacgagg
     2041 acgcggcccg ttcgctgggc aagaacgtct acgcgttcaa gatgcagagc ctgctgatcg
     2101 gcggcgcgat cggcggcgtc gcgggcatgc tcctcgcggt cgaccgccag ttcgtcgacg
     2161 cgcagcagtt cgtcgccaac gtcacgttcc tcgcgtacgc cgccctcatc ctcggcgggc
     2221 tgggccgcat cctcggcccg gtcgtcgggt cgatggtgct gtggttcttc gtgagcgcgc
     2281 tgaatcagtt cctgcgcgaa gcggtggcca acgacttcct cggcatcacc cacgtcatgg
     2341 acgagaacga cgtcggcgcg gtgcgcttca tcctcgtggg cgtgctcatc atggtgctcg
     2401 tcatcttccg accacagggc atcctcggat cgcgctcgga ggtgatgctc ggtgacggct
     2461 gagcagctca cgaccgacac gacgcccatg ctgcgcgttt ccgggttgcg ccgcaccttc
     2521 ggtggtctca ctgcggtcga cgtcgacgag ttgcagatca agcgcaacac gatcaccgcg
     2581 ctcatcggtc ccaacggcgc gggcaagacg acgatgttca acctgctgac cgggttcgac
     2641 aagcccgacg gcggggcgtg gtggttcgac ggtcaggaca tgagcgggct gtccgcgtac
     2701 cgcgtcgccc gcctcgggat ggtccgcacg ttccagctga ccaagtcgct cgcgcggctc
     2761 acggtgctcg agaacatgct gctcggcgcg cccggccagc agggcgagcg cctcttgccc
     2821 gcgctgttcc acacctggcg cgggcaggag cgcgccaaca ccgggcgcgc ccgcgcgctg
     2881 ctcgagcgct tcaagctcgc ggacaaacaa cgagatctcg cgggcacact gtcgggtggg
     2941 cagcgcaagc tgctcgagat ggcgcgcgcg ctgatggtgg acccgaagct gatcatgctc
     3001 gacgaaccga tggccggcgt gaaccccgcg ctcgtacagt cgctcctcga gcacatcaag
     3061 gagttgcggg ccgaaggccg cacggtcgtc ttcgtggagc acgatatgga cgtcgtcatg
     3121 aacatcagcg actgggtcgt gtgcatggcc gaaggtcacg tcatcgccga agacgtcccc
     3181 gcgcggatca tcgccaacga agcggtcatc gaggcgtacc tcgggctgcg gcacggagac
     3241 gcatcgtgaa cctgctcgaa gcgcgcgacg tcgtcgccgg gtacctcccg ggcatcgaca
     3301 tcctgcacgg ctgcaacctc gacgtcggcg agggcgaact ggtcggcatc atcgggccca
     3361 acggcgcggg caagtcgacg ttgatcaagg cgatcttcgg cctcgtcacc gtgcggtcgg
     3421 gcaccatcg
//
LOCUS       DASUGA010000688         3270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_450543, whole genome shotgun sequence.
ACCESSION   DASUGA010000688 DASUGA010000000
VERSION     DASUGA010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450543"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1313)
                     /locus_tag="VFX21_13700"
     CDS             complement(<1..1313)
                     /locus_tag="VFX21_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX5097074.1"
                     /translation="MAVRGSLVERWVPGVRASRTYRRSWLRSDAVAGLVLAAILVPQG
                     MAYAELAGLPPVTGLYTTVACLLTYAVFGPSRVLVLGPDSSVSPLILAAITPLFVAGD
                     TGEAIALAGMLAVIVGLIEIGLGIGKLGFVADLLSNEVQVGYMNGLAVTIIVGQLPKL
                     FGFSTDADTFIGDVREFVRNVDETIGAALVVGLAVLAVLVVLPRISRRVPAVLVAVVG
                     ATIASAVLDLANDGVQTVGSLPEGFPKPSIPWTNFDDVGALLIAAIGITLVSLTDTIA
                     TASSFAARRGDEVDPDQEMVAIGASNLAAGLVQGFAVSTSGSRTAVAEQSGAKSQAAG
                     VVGASVVVLLLVFFSSLLADLPQSALAAIVIMAAVSLVDIKALLHYRRVRISAFLLSL
                     VATGGVIFFGVLEGIVIAIGLAIIMFFRRSWWPYGVVVGRVDGMGG"
     gene            1352..1723
                     /locus_tag="VFX21_13705"
     CDS             1352..1723
                     /locus_tag="VFX21_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HEX5097075.1"
                     /translation="MIEAITGLPDGVLGFVFTGHVTKDEYAAVLAPPLKERIDKKQKI
                     RLLIEIDNGFDRFEAGAFWEDLKFGLGSGLTHLSAWERMALVSDEDWVHRAISLFGWL
                     VPGEVKVYPLDQRGEAIAWLG"
     gene            complement(1720..2058)
                     /locus_tag="VFX21_13710"
     CDS             complement(1720..2058)
                     /locus_tag="VFX21_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097076.1"
                     /translation="MNRNFSQTITIRTKNPDEIVKMLAEWDVNQATCDIMGYMGTRML
                     ADREVQDQYVIIADFGVVDPNVSAAEEAARNNDRPETREWARKLLEVIEGEPEYHHFD
                     EIYRTDPTGR"
     gene            complement(2055..3146)
                     /locus_tag="VFX21_13715"
     CDS             complement(2055..3146)
                     /locus_tag="VFX21_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097077.1"
                     /translation="MDTGRAPGVDLHDAAEVQLVARGIATAVAPEPGLTDVQADLLEA
                     IASALTGVEVDYRALEPLSREELADVLASRDAEYRHRIVHHMVLAEMVLRPIPVVVAH
                     RVAQYAQALGVDDDFVRVARRYAQGAYGLAWIDLQRNGFVEHVQEANDGSTPAPVPEM
                     FEPGEVDPALAARWQAFAELPAESLGRQVWEMYDSRGFALPGTPGGAPAYLAQHDFVH
                     VLADYGTNLKGEMEVFALIGRSDPDPKGFAWLATLIGLFETGYIKSTGFFDRDTTERN
                     IRAPGMHQRIADAIRRGKAVCGAYGVDLFDVDYDALAARPVEEVRILLCIPPKSSGAV
                     EGGSAGLFELAGMSEHQRQVVAQRRGGKQ"
BASE COUNT          558 a         1116 c         1107 g          489 t
ORIGIN      
        1 ccgcccatcc cgtcgacgcg cccgacgacg acgccgtacg gccaccagct ccgccggaag
       61 aacatgatga tcgccaaccc gatcgcgatg acgatgcctt ccaacacgcc gaagaagatg
      121 acaccgccgg tcgcgaccaa cgacagcagg aacgcgctga tgcgaacccg gcggtagtgc
      181 agcagggcct tgatgtcgac caacgacacc gcggccatga tcacgatcgc ggccagcgcc
      241 gactgcggca ggtcggccag caagctgctg aagaacacga gcagcagcac gacgacgctc
      301 gcgccgacga cgccggccgc ttggctcttc gcgcccgatt gctcggcgac cgcggttcgc
      361 gagccgctgg tggagaccgc gaagccctgt acgagaccgg cggcaaggtt cgaagcgccg
      421 atcgcgacca tctcctgatc ggggtcgacc tcgtcgccgc gccgcgccgc gaagctcgac
      481 gcggtcgcga tcgtgtcggt gagcgacacc agcgtgatgc cgatggcggc gatcaagagg
      541 gcgccgacgt cgtcgaagtt cgtccacggg atcgacggct tcgggaatcc ttcgggcaac
      601 gacccgacgg tctgcacacc gtcgttggcc agatcgagca ccgccgacgc aatcgttgcg
      661 cccaccaccg cgacgaggac ggcaggcacg cggcggctga tccgtggcag cacgacgagg
      721 actgcgagca cggccagccc gacgaccagc gcggcgccga tcgtctcgtc gacgttgcgc
      781 acgaactcgc gtacgtcgcc gatgaacgta tcggcgtcgg tcgagaagcc gaacagcttc
      841 gggagctgac cgacgatgat ggtcaccgcg aggccgttca tgtaaccgac ctgcacctcg
      901 ttcgacagca ggtcggcgac gaatccgagc ttgccgatgc cgaggccgat ctcgatcagg
      961 ccgacgatga ccgcgagcat cccggccagt gcgatcgcct cgcctgtgtc gccggcgacg
     1021 aacagcggcg tgatcgcagc gaggatcagt ggcgacaccg acgagtcggg gccgagcacg
     1081 aggacgcgcg acggtccgaa gaccgcgtac gtgagcaggc acgcaacggt cgtgtagagc
     1141 ccggtgacgg gcggcagccc ggccagctcc gcgtaggcca tgccctgcgg cacgaggatg
     1201 gcggcgagca cgagacccgc cacggcatcg gatcgcagcc acgaacgccg gtacgtgcgc
     1261 gaggcgcgca caccgggcac ccaccgttcg accaacgatc cccgcacggc cacgcgagaa
     1321 acctaaacgc gggagcggta tgttcgcgac catgatcgag gcaatcaccg gcctgcccga
     1381 cggcgtgctc ggattcgtct tcacgggtca cgtcacgaag gacgaatacg ccgcggtgct
     1441 ggcaccgccg ttgaaggaac gcatcgacaa gaagcagaag atccgtctgc tcatcgagat
     1501 cgataacggc ttcgaccgct tcgaagcggg cgcgttctgg gaggacctca agttcgggct
     1561 cggttcgggg ctcacgcacc tgtccgcgtg ggaacgcatg gcgctcgtgt ccgacgagga
     1621 ctgggtgcac cgcgccatct cgctcttcgg gtggctggtg cccggtgaag tgaaggtcta
     1681 cccgctcgac caacgcggtg aagcgatcgc ctggctcggc taacgccccg tcggatcggt
     1741 gcggtagatc tcgtcgaagt ggtggtactc gggttcgccc tcgatgacct cgagcagttt
     1801 ccgcgcccac tcgcgcgtct cgggccggtc gttgttacgc gccgcttctt ccgccgctga
     1861 cacgttcggg tcgacgacgc cgaagtcggc gatgatcacg tattgatcct gcacttcgcg
     1921 gtcggcaagc atgcgcgtgc ccatgtagcc catgatgtcg cacgtcgcct ggttgacatc
     1981 ccactccgcc agcatcttga cgatctcgtc cgggttcttc gtgcgaatcg tgatcgtctg
     2041 gctgaagttg cggttcattg ctttcctccg cggcgctgcg cgacgacctg ccgttgatgc
     2101 tcggacatgc cggcaagttc gaacagaccg gcggatccgc cttcgacggc gccggacgac
     2161 ttcgggggga tgcacaacaa gatgcggacc tcttcgacgg gacgcgcggc cagcgcgtca
     2221 tagtcgacat cgaagaggtc gacaccgtag gcgccgcaca ccgccttgcc gcgccggatg
     2281 gcgtcggcga tgcgttggtg catgccgggg gcccggatgt tgcgctccgt cgtgtcccgg
     2341 tcgaagaacc cggtgctctt gatgtagccc gtctcgaaga ggccgatcag tgtcgcgagc
     2401 cacgcgaagc ccttcgggtc gggatccgac cgcccgatga gcgcgaacac ctccatctca
     2461 cccttgaggt tggtgccgta gtcggcgagc acgtgcacga agtcgtgctg ggccaggtac
     2521 gcgggcgcac cgccgggcgt accgggcagc gcgaacccgc ggctgtcgta catctcccac
     2581 acctgccggc ccagcgactc ggcgggcagt tcggcgaacg cctgccagcg ggccgcgagc
     2641 gcgggatcga cttcacccgg ctcgaacatc tcgggcaccg gcgccggcgt gctgccgtcg
     2701 ttcgcctcct gcacgtgctc gacgaacccg ttgcgctgga ggtcgatcca agccagcccg
     2761 tacgcaccct gcgcgtaccg gcgcgcgacg cgcacgaagt cgtcgtcgac gccgagcgcc
     2821 tgggcgtact gcgcgacgcg gtgggcgacg acgacgggga tcggccgcag caccatctcc
     2881 gcgagcacca tgtggtggac gatacggtgt cggtattcgg catcgcgcga cgcgaggacg
     2941 tcggccagct cttcgcggct cagcggctcg agcgcccggt agtcgacctc gacgccggtg
     3001 agggcgctcg cgatcgcttc gagcagatcg gcctgcacat cggtcaggcc cggctcgggc
     3061 gccaccgccg tcgcgattcc acgcgcgacg agctgcacct cggcggcgtc gtggaggtcg
     3121 acgccggggg cgcggccggt gtccatgccc gaaaggctat tgccatcccg gagggtgaga
     3181 gggttcgacc ggctcctccc gggagacgca acatggcggg catgcccgag ccgcagtgca
     3241 acgaactcgc cttctacgtc ctggccgggg
//
LOCUS       DASUGA010000689         5511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2014772, whole genome shotgun sequence.
ACCESSION   DASUGA010000689 DASUGA010000000
VERSION     DASUGA010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2014772"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VFX21_13720"
     CDS             <1..597
                     /locus_tag="VFX21_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018638396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5097078.1"
                     /translation="GSQRDDLLSHFLTVEIDGDRLAREEILDICVLFLIAGLDTVTAS
                     LDCFFAQLAEDTRTRHRLVEAPDEIPTMVEEMLRWQTPVMGAARIAARDATIGDFAVN
                     RGEQVMTILGAANVDPAEFADADALVWDRTTNRHLAFGGGIHRCLGSHLARLELRVAL
                     REWHLRIPDYRIKPGAELQFSPGIRSVDHFPMLLGREP"
     gene            606..2282
                     /locus_tag="VFX21_13725"
     CDS             606..2282
                     /locus_tag="VFX21_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5097079.1"
                     /translation="MLDELGLLASAIAGRSLTVVPADPAEASWTDGTTVFVETSLADP
                     VEVVVVQASLLAAGSLRPELVSQLARRPALARRYLAFEGHRALMANDFVPPRYRALAN
                     RTVADDLADARASLARARTKQPVVGAPASFGTIHAQAVLTAAERIEPAPATTVASRPL
                     PALAEPGDETGDLGDLLSSPVGGGGAVGRLFRKLLSPTRGRAGGGPPGADAPTHFSTG
                     RPGTSHPVASSGPARALDDTAGAAHTGRAYPEWDVHRARYRPDWCTVSEVDAPARDTP
                     PRPPPDAIAFRRPLARLGLGLTNCRRRRQGDDVDLDAAVEAHVDARAGALHDEVYIES
                     LRRRRDLSVLVLLDVSGSAAEPGTARTTVHEHQVRAATALTAALESLGDRVALYGFNS
                     RGRHHVQLLRVKAFDDHLDGRVGTRLASLTPGAYTRLGAAIRHATTLVEQRAGTPRRL
                     LVVLSDGFAYDHGYEGRYGEADARRALVEAGRRGVGCLCISVGGSTDPAALRRVFGAA
                     AYAAVPEIAQLAPVIGPLFRAALQSADVQRRTFRRDERTRERLHPERSMG"
     gene            2279..3070
                     /locus_tag="VFX21_13730"
     CDS             2279..3070
                     /locus_tag="VFX21_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HEX5097080.1"
                     /translation="MTTARPYYLPVADEEHIFKAACTQNLAVMLKGPTGCGKTRFVEA
                     MAHDLDRPLITVSCHDDLTTADLVGRYLLEGGDTRWVDGPLTRAVREGAICYLDEVVE
                     ARQDTTVVIHPLADHRRQLPIERLGVVLDAAPAFCLVVSYNPGYQSVLKDLKDSTRQR
                     MVAIEFDFPPADIEEKIVAHEAAIEPAGAARLVQLAQAIRRLESAGLREVASTRVLIA
                     AARLHADGVPLNAAARAAIAGPLSDDPSVGAGLREVIAAYLPADD"
     gene            3102..4214
                     /locus_tag="VFX21_13735"
     CDS             3102..4214
                     /locus_tag="VFX21_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5097081.1"
                     /translation="MAYESTAQPIKLGYLFDFVLPDAYPQEMRADLTQSFELVFEQGL
                     AQGIIDRPVEIVFREVEGLPKGTVKAVIDAYGELVDEGCLAIFGPSITDNCVPTREAI
                     EERFHVPAISVTGSEEWLGEWTFSLSMGSMTDEPIFWAHLLAQRGLNDVGVLVESSLV
                     GDSYLRSFRSACAAKGIRIVAEEQIAQTAQDIGDAVRALHEAKVQAVVHCGFGFGVVL
                     IGPVLDELGWDPPRFMGTSFQNAWINEIVWNAIVGWTGIDQYDDGNQVGQEFLDQYAA
                     RYGRRPEYCVPVVNRDLAMVLLHAFADARPLSPRGVRDALERVKLLPAASGSPGTRIS
                     FGRYGHRGWMGAGYLVARRLDPDGRHAHLVERFGQD"
     gene            4218..5354
                     /locus_tag="VFX21_13740"
     CDS             4218..5354
                     /locus_tag="VFX21_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spirocyclase AveC family protein"
                     /protein_id="HEX5097082.1"
                     /translation="MTAQLAPPLGDVRPPDTPTRRTNVVGWLGLAALVGFVLFVAVNA
                     SSGADASRIENPNVTGTPRPVAPLFGFDHWIALHQIGTVVMMVALVTACVVGWRRSPR
                     HPVLLMVLVTTILVWQDPIMNWAPYAVYNPQLWHWPVTWPLVSLSPTVEPFIVVGYAT
                     FYMAPFFPAVVVLRRLQRRAPLESFVWKHPLLCLAGLILAFGFVFDAMLEIFLVRTQL
                     YIYSQVIPFGSFAAGQSYQFPLIWESALVCTVMIPAGVLLYRDDTGRTTAEKLAYRVR
                     SFRTRPALGTFAVMFLIINAAYFFLYGGGFWLIRVTKSATSVACPYPFPEAKTYDPNA
                     FYEENGQQGPFFPGIWSAWPSGQPDGRPTVDPPADGGRCGSADD"
BASE COUNT          790 a         2030 c         1816 g          875 t
ORIGIN      
        1 ggttcgcagc gcgacgatct cctcagccac ttcctgaccg tcgagatcga cggcgaccgg
       61 cttgctcgcg aagagatcct cgacatctgc gtgctgttcc tcatcgcggg cctcgacacc
      121 gtgaccgcgt cgctggactg cttcttcgcg cagctcgccg aggacacgcg tactcggcac
      181 cgtctcgtcg aagcgccgga cgagatcccg acgatggtcg aggagatgtt gcggtggcag
      241 acgcccgtga tgggtgcggc gcggatcgca gcacgggatg caaccatcgg cgactttgcc
      301 gtcaaccgcg gtgagcaggt gatgacgatc ctcggcgccg cgaacgtcga ccctgcggag
      361 ttcgccgacg ccgacgcgct cgtgtgggac cgcaccacca accgtcacct cgccttcggc
      421 ggcggcatcc accgctgcct cggttcgcac ctcgcgcgcc tcgagctgcg cgtcgcgctc
      481 cgcgagtggc acctgcgcat ccccgactac cggatcaagc ccggtgcgga gctccagttc
      541 tcccccggga tccgcagcgt cgaccacttc ccgatgctgc tcggtcgcga gccctgagcg
      601 cgccagtgct cgacgagctc ggtctccttg cgtccgcgat cgccgggcgg tcgctcaccg
      661 tcgtgcccgc cgatcccgcc gaggcatcgt ggaccgacgg caccacggtg ttcgtcgaga
      721 cgtcgctcgc cgatccggtc gaggtcgtcg tcgtccaggc atcgctgctc gccgcgggca
      781 gcctgcggcc cgagctcgtc tcgcaacttg cgcgtcgtcc cgcgctcgcc cggcgctatc
      841 tcgcgttcga gggccaccgc gcactcatgg ccaacgactt cgtcccgccg cgctaccggg
      901 cgctcgccaa ccgcaccgtc gcggacgatc tcgcggacgc gcgtgcctcg ctggcgcgcg
      961 cccgcaccaa gcagccggtc gtcggcgcac ccgcgtcgtt cggcacgatc cacgcgcaag
     1021 ccgtactcac cgcggccgaa cgcatcgagc ccgcgcccgc aacgacggtc gcctcgaggc
     1081 cattgccggc gctcgcagaa ccgggcgacg agaccggcga tctcggcgac ctgctgtcga
     1141 gcccggttgg cgggggtggc gccgtcgggc gcctgtttcg caagctgttg tcaccgacgc
     1201 gcggccgcgc cggcggtggc ccgcccggcg ccgacgcgcc gacccacttc tcgaccggac
     1261 ggccgggaac gagtcacccc gtcgcgtcgt ccggacccgc gcgcgcactc gacgacacgg
     1321 ccggggccgc ccacaccggc agggcgtatc ccgaatggga cgtccaccgg gcgcgctatc
     1381 ggcccgactg gtgcacggtg tcggaggtcg atgcgcccgc gcgtgacact cccccgcggc
     1441 cgccgcccga cgcgatcgcg ttccgccgtc cgctcgctcg gctcgggctc gggctcacga
     1501 actgccgccg gcgccgtcaa ggtgacgacg tcgatctcga cgcggccgtc gaagcccatg
     1561 tcgacgcgcg cgcgggcgca ctccacgacg aggtctacat cgagagcctg cgccggcgcc
     1621 gcgacctgtc ggtcctcgtg ctgctcgacg tgtccggctc ggcggccgag cccggcaccg
     1681 cgcgtacgac ggtgcacgag caccaggtcc gcgccgcgac ggccttgacc gccgcgctcg
     1741 aatcgctcgg cgatcgcgtc gcgctctacg gcttcaactc gcggggacgt catcacgtgc
     1801 agctgctgcg cgtgaaagcg ttcgacgacc acctcgacgg acgggtgggc acccggctcg
     1861 cgtcactcac gccgggcgcg tacacccgcc tcggcgccgc gatccgtcac gcgacaacgc
     1921 tcgtcgaaca gcgcgcgggc acgccgcggc gactgctcgt cgtgctgtcg gacggcttcg
     1981 cgtacgacca cggctacgaa ggccgctacg gcgaggccga cgcgcgacgc gcgctcgtcg
     2041 aagccggccg ccgcggtgtc gggtgcctgt gcatcagcgt gggcggctcc accgaccccg
     2101 ccgcgctgcg ccgtgtgttc ggcgccgccg cgtacgcggc cgtgcccgag atcgcgcagc
     2161 ttgcccccgt catcggaccg ctgttccggg ccgcactgca gtcggccgac gtacaacgac
     2221 gcacgttccg gcgcgacgaa cgaacgcggg aacgcctgca ccccgagagg agcatgggat
     2281 gacgacggcc cggccgtact acctgcccgt cgcggacgag gagcacatct tcaaggccgc
     2341 gtgcacgcag aacctcgcgg tgatgctgaa aggtccgacc gggtgcggca agacgcgctt
     2401 cgtcgaggcg atggcgcacg acctcgaccg tcccctcatc accgtgtcgt gtcacgacga
     2461 tctcacgacc gccgacctcg tcggtcgcta cctcctcgag ggcggcgata cccgctgggt
     2521 cgacggaccg ttgacgcgcg cagtgcgcga aggcgcgatc tgctacctcg acgaagtcgt
     2581 cgaggcgcgc caggacacca cggtggtcat ccacccgctc gccgaccacc gacgtcaact
     2641 gccgatcgaa cgactcggtg tggtgctcga cgccgcgccg gcgttctgcc tggtcgtctc
     2701 gtacaacccc ggttaccaga gcgtgctgaa ggatctcaag gactcgacgc gccaacgcat
     2761 ggtcgcgatc gagttcgact tcccgcccgc cgacatcgaa gagaagatcg tcgcgcacga
     2821 agccgccatc gaaccggccg gcgccgcgcg actcgtgcaa ctcgcgcagg cgattcgccg
     2881 gctcgaatcg gccggactgc gcgaagtcgc gtcgactcgg gtcctcatcg ccgccgctcg
     2941 cctccacgcc gacggtgtgc cgctcaacgc ggcggcacgc gccgcgatcg cagggccgct
     3001 gagtgacgac ccgagcgtcg gcgcggggct gcgcgaggtc atcgccgcgt acctgccggc
     3061 cgatgattga ccacctgcgg aacaactatt aggttcgatc cgtggcctac gagagcacgg
     3121 cccagccgat caagctcggg tacctcttcg acttcgtgct gcccgacgcg tatccgcaag
     3181 agatgcgcgc cgacctgacc caatcgttcg agctcgtctt cgagcagggc ctcgcgcaag
     3241 ggatcatcga ccgtccggtc gagatcgtct tccgcgaggt cgagggcctg cccaagggca
     3301 cggtcaaggc cgtgatcgac gcgtacggcg agctcgtcga cgagggctgc ctcgcgatct
     3361 tcggcccgtc gatcaccgat aattgcgtgc cgacacgcga agcgatcgag gaacgcttcc
     3421 acgtccccgc gatcagcgtc accggatccg aggagtggct gggcgagtgg acgttctcgc
     3481 tctcgatggg ctccatgacc gacgagccga tcttctgggc gcacctgctc gcccagcgcg
     3541 gcctcaacga cgtcggcgtc ctcgtcgaga gctcactcgt cggtgacagc tacctccgct
     3601 cgttccgcag cgcatgcgcg gcgaagggca tccgcatcgt cgccgaagag cagatcgctc
     3661 agaccgcaca agacatcggc gacgcggtcc gcgcgctgca cgaagccaag gtccaagctg
     3721 tcgtgcactg cggcttcgga ttcggtgtcg tgctcatcgg ccccgtgctc gacgaactgg
     3781 gatgggatcc gccgcgcttc atgggcacgt cgttccagaa cgcgtggatc aacgagatcg
     3841 tgtggaacgc gatcgtcggc tggaccggca tcgaccagta cgacgacggc aaccaagtcg
     3901 gccaggagtt cctcgaccag tacgcggcgc gttacggccg gcgacccgag tactgcgttc
     3961 ctgtcgtgaa ccgcgatctc gcaatggtgc tgttgcacgc ctttgccgac gcgcgcccgc
     4021 tctcaccgag aggcgtgcgc gacgcgttgg agcgggtgaa gctcctgccc gcggcgtccg
     4081 gttcgcccgg cacccgcatc tcgttcggtc gctacgggca ccgtggctgg atgggcgcgg
     4141 gctacctcgt ggcccgccgg ctcgatcccg atggtcggca tgcgcacctc gtcgagcgct
     4201 tcgggcagga ctgagcggtg accgcgcagc tggcgccgcc gctcggggac gtgcgcccgc
     4261 cggacacacc cacgcgccgc accaacgtcg tcggctggct cggcctcgcc gcgctcgtcg
     4321 gcttcgtgct cttcgtcgcg gtcaacgcga gctcgggcgc ggacgcctca cggatcgaga
     4381 acccgaacgt gacgggcacg ccgcggccgg tcgcgccact cttcggcttc gaccactgga
     4441 tcgcgctgca ccagatcggg accgtcgtca tgatggtcgc gctcgtgacc gcgtgcgtcg
     4501 tcggctggcg ccgctccccg cgccacccgg tgctgttgat ggtgctcgtg accacgatcc
     4561 tggtgtggca ggacccgatc atgaactggg cgccgtacgc ggtctacaac ccgcagttgt
     4621 ggcactggcc cgtgacgtgg ccgctggtgt cgctgtcgcc gaccgtcgag ccgttcatcg
     4681 tcgtcgggta tgcgaccttc tacatggcgc cgttcttccc cgcggtcgtg gtgctgcgcc
     4741 gtctgcaacg ccgcgcgcca ctcgagtcgt tcgtgtggaa gcacccgctc ctgtgcctcg
     4801 cgggtctcat cctcgcgttc ggcttcgtct tcgacgcgat gctcgagatc tttctcgtgc
     4861 gcacgcagct ctacatctac tcgcaggtga tcccgttcgg ctccttcgcg gcgggccagt
     4921 cgtaccagtt cccgctcatc tgggaatcgg cactggtgtg cacggtgatg atcccggccg
     4981 gtgtgctgct ctatcgcgac gacaccgggc gcacgactgc cgagaaactc gcctatcgcg
     5041 tgcgctcctt ccgcacaagg cccgcgctcg gcacgttcgc agtgatgttc ctgatcatca
     5101 acgccgcgta cttcttcctc tacggcggcg ggttctggct catccgcgtg acgaagagcg
     5161 cgacgtcggt cgcgtgcccg tatccgttcc cggaggcgaa gacgtacgac cccaacgcgt
     5221 tctacgaaga gaacgggcag caagggccgt tcttccccgg tatctggtcg gcgtggccga
     5281 gcgggcaacc cgatggtcgc ccgaccgtcg acccacccgc cgacggcggg cgctgcggga
     5341 gtgcggatga ctgagttcga ccagtgcttc gagatcacgt ccgtcgcgta tcgggcggac
     5401 tgacgccggc acctcgcaac gtcgtctcgc gcgcgagttc gagcgcgcgg cgcgggccca
     5461 gcttgcccac gatgttctcg ggcatccaca acaactcgaa cagcgcccac g
//
LOCUS       DASUGA010000690         2309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1874749, whole genome shotgun sequence.
ACCESSION   DASUGA010000690 DASUGA010000000
VERSION     DASUGA010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1874749"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VFX21_13745"
     CDS             <1..1011
                     /locus_tag="VFX21_13745"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylic ester hydrolase"
                     /protein_id="HEX5097083.1"
                     /translation="HRELMVQLWYPATHEPNAPRAAYMPDADAVGPVLARFLGVPAVT
                     LAPLRDFTTNAVARAPLADDGRAYPVIVLLVGIKGSYRQLHTFQVEELVSRGYVVAAL
                     DQPTSVAMVVFPDGREVAYDDRWDPPHSAFMDANIPYLAHDVSFTLDQLAAIAESNHL
                     LHGRLDLERVGIIGHSFGAVVGAEACHLEPRLRAGLLEEAFVPADVLRAGLRQPIMFF
                     TRDEDSMRLERRTAGGWPETDIRETLDTMWAAYRQLPGDGFFVQARNMFHLDMTDAGL
                     LAPFVPWPGLAGPIGVERAHEIVNAYSLAFFDRELRGQPAPLLDGPPERFTEVQFESR
                     GS"
     gene            complement(1152..1631)
                     /locus_tag="VFX21_13750"
     CDS             complement(1152..1631)
                     /locus_tag="VFX21_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097084.1"
                     /translation="MRSRIASSAALATLGGALLFGACSDSSPSGDREAKIVAAAIRAL
                     VLPTNDETKVTRTVFVGAPEQPGRVSLDLQADVLNELDEFESLRFVDARAEAIEDDLS
                     RRVHGDGVYLEIGPVPETGSRIVVPALHYLDRNHQSRLRLHMARSGSTWSVTSIDGL"
     gene            1702..>2309
                     /locus_tag="VFX21_13755"
     CDS             1702..>2309
                     /locus_tag="VFX21_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5097085.1"
                     /translation="MRGVVRVFGPEPLSALVRVHSISTAADACITVSLAGSLFFAVSV
                     DAARPRLALYLLLTLAPFAIVAPLIGPVTDRFRGAAPAVIAVTGQARAILALFIAIDL
                     RGLLLYPESFAVLVLGRSYSIVKRALVPELVPDANDLVAANARLSRVGSVSGALGGAL
                     AVAVFQASGPTAVLRLAAILHVVGALLAFRIPSTSAVPETVSA"
BASE COUNT          324 a          820 c          765 g          400 t
ORIGIN      
        1 catcgggagc tgatggtgca actctggtat ccggcgacgc atgaaccgaa cgcgccgcgt
       61 gctgcgtaca tgccggatgc cgacgcggtc gggcccgtcc tcgcgcgctt cctcggggtg
      121 ccggccgtga ccctcgcgcc attgagagac ttcaccacca acgcggttgc gcgtgcgccc
      181 ctcgccgacg acggacgcgc gtacccggtg atagtgctgc tcgtggggat caagggcagc
      241 taccggcaac tgcacacgtt ccaagtcgaa gagctggtgt cgcgcggcta cgtcgtcgcc
      301 gcgctcgatc agccgacttc ggtggccatg gtcgtgtttc ccgatggacg cgaggtcgcg
      361 tacgacgacc gttgggatcc gccgcacagc gcgttcatgg acgcaaacat tccctacctt
      421 gcgcacgacg tgtcgttcac gctcgatcag ctcgcggcga tcgcggaatc gaatcatctg
      481 ctgcacggac ggctggatct cgaacgtgtc ggcatcatcg ggcactcgtt cggagcggtc
      541 gtcggagccg aggcctgcca cctcgaaccg cgtctgcgcg cgggtctgct cgaggaagcc
      601 ttcgtgccgg cggacgtcct gcgcgccggg ttacggcagc cgatcatgtt cttcacgcgg
      661 gacgaggaca gcatgcggct cgagcggcga accgcgggcg gctggccgga gaccgacatc
      721 cgcgagacgc tcgacacgat gtgggccgcg taccggcagc tcccgggcga cggcttcttc
      781 gtccaggcgc gcaacatgtt ccatctcgac atgaccgacg ccgggctcct ggcgccgttc
      841 gtgccctggc ccggcctcgc cggaccgatc ggcgtggaac gcgcgcacga gatcgtcaac
      901 gcatactcgc tcgcgttctt cgaccgggag ctccgcgggc agccggcacc gctcctcgac
      961 ggcccgccgg aacgattcac cgaggtgcag ttcgaatctc gcggttcgtg agtcggcgcc
     1021 ccaacctctc ggggaacagc gcggttgggg accgatgtcg tcgcgtcacg cacgctgacg
     1081 acggcgtggg gcgacacggc gattcactga tccgaaggcc ggtgcccggt ggatgtactg
     1141 ccggtacacg gtcataaccc gtcgatcgac gtcacgctcc acgtcgaacc cgacctcgcc
     1201 atgtgcagcc ggagccgcga ctggtgattc cgatcgaggt agtgcagcgc ggggacgacg
     1261 atgcgcgagc ccgtctcggg caccggacct atctcgaggt agacgccgtc accgtgcacg
     1321 cgacgcgaca ggtcgtcttc gatggcttcc gcccgcgcgt cgacgaagcg cagcgactcg
     1381 aactcgtcga gctcgttgag cacgtccgct tgcaggtcga gcgacactcg gcccggctgc
     1441 tcgggtgcgc cgacgaacac cgtgcgcgtc accttcgtct cgtcgttcgt cggaaggacc
     1501 agcgcgcgta tcgcggcggc gacgatcttc gcctcccggt caccgctcgg cgacgaatcg
     1561 ctgcacgccc cgaacaggag agctccaccc aaggtcgcca gtgccgccga ggaggcgatc
     1621 cgcgatcgca ctcgcgtcat ctcatccctt cgggagacgt tggttcagcg ggctgcgccg
     1681 cgactggaag cataggaacc cgtgagaggc gtcgtgcgtg tgttcggacc cgagccactc
     1741 tctgcgctgg tgcgagtgca ctcgatcagc accgcggccg acgcctgcat caccgtttcg
     1801 ctcgcaggct cactgttctt cgccgtctcg gtcgacgccg ctcggccgcg gctggcgctg
     1861 tacctgctgc tcaccctcgc ccccttcgcg atcgtggcgc cgctcatcgg accggtgacc
     1921 gaccggttcc gcggtgccgc cccggcggtc atcgccgtca ccggtcaagc ccgtgcgatc
     1981 ctcgcactgt tcatcgcgat cgatctgcgg ggcctgttgc tctaccccga gtcgttcgcg
     2041 gtgctcgtgc tcggccgctc gtactcgatc gtcaagcgcg cgctggtgcc cgagctggtg
     2101 cccgatgcca acgacctggt cgccgccaac gcgcggctct cacgcgtcgg ttcggtctcg
     2161 ggtgcgctcg gcggcgcgct cgcggtcgcg gtgtttcagg cgagcgggcc gaccgcggtc
     2221 ctgcgcctgg ccgcgatcct gcatgtcgtc ggtgcgttgc tcgccttccg catccccagc
     2281 acctctgccg tgcccgagac ggtctcggc
//
LOCUS       DASUGA010000691         2247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_68601, whole genome shotgun sequence.
ACCESSION   DASUGA010000691 DASUGA010000000
VERSION     DASUGA010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68601"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VFX21_13760"
     CDS             <1..387
                     /locus_tag="VFX21_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HEX5097086.1"
                     /translation="NAIDRSSDTIRGRAEFKNVDGRLTPGMFGRIQIATSAPAEALLV
                     PDTAIGTEQVRKFVYVLNADNVATPKYVTLGQLVDGLRVVQGLKPDDTIIIDGLMRVR
                     PGAKVAPQQAEAPTGSVRDTSSIRTN"
     gene            397..>2247
                     /locus_tag="VFX21_13765"
     CDS             397..>2247
                     /locus_tag="VFX21_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5097087.1"
                     /translation="MRFSHFFIDRPIFAAVISIVFVILGAVAVGRLPIAQYPDIAPPV
                     VNVTGQYPGASAEVVASTVVAPLEQQINGVEHMLYISSNSTVDGRFSISVTFDLGTDL
                     DTAQVQVQNRVAIAQPRLPADVRNIGVTVAKASPDLMMVVHLYSPDQSRDTLFISNYA
                     SVNVVDVLSRIQGVGSITVFGSRDYSMRVWLDPDRLQSLGLTANDVTTALQRQNVQVA
                     AGILNQPPVKQPGAFQISVQTQGRLADPNEFSQIVVKQSGDAVVRLKDVARVELAALD
                     YGVNSYLDKYPATGLGVFQLPGSNAIETAEKIKSTMEELSKSFPKGLKYDIIYNPTDF
                     IQQSVDAVVTTILEAVVLVVLVVILFLQTWRAAIIPIVAIPVSLIGTFFLMSLFGFSL
                     NNLSLFGLVLAIGIVVDDAIVVVENVERNMAAGMSPREAAYRTMEEVGAALVAIALVL
                     SAVFVPSTFITGISGQFYRQFALTIAGATVISLVVSLTLSPALCALLLKPHSEKHKTP
                     WYMWPVEAFFRAFNWSFEKASHGYAWMVGRLVRLSVLMLIVYVGIIIFGMNEFRKTPV
                     GFIPAVDGGYLITVTQLPPAASLDRTDAVNQRVVEMALETPGVAHAVNIVG"
BASE COUNT          368 a          774 c          679 g          426 t
ORIGIN      
        1 aacgccatcg accgctcgtc cgacaccatc cgcgggcggg ccgagttcaa gaacgtcgac
       61 ggccgcctga ctcccggcat gttcggccgc attcagatcg ccacctcggc cccggccgag
      121 gcgctgcttg ttcccgacac cgccatcggc accgagcagg tccgcaagtt cgtgtacgtg
      181 ctcaacgcgg ataacgtggc gacgccgaag tacgtcacgc tcgggcagct cgtcgatggc
      241 ctgcgcgtcg ttcagggatt gaagcccgac gacacgatta tcatcgacgg cctgatgcgc
      301 gtgcgtccgg gcgccaaggt cgcgccgcag caggccgaag ccccgacggg cagcgtccgc
      361 gacacgtcct ccatacgcac caactaaagg acggcgatgc gtttttcgca cttcttcatc
      421 gatcggccga tcttcgcggc cgtcatttcg atcgtgttcg tgatcctcgg cgccgtcgcc
      481 gtgggtcggc tgccgatcgc ccagtatccc gatatcgcgc cgccggtggt caacgtcacc
      541 ggccagtatc cgggcgcaag cgcggaggtc gtcgcttcca ccgtcgtcgc gccgctggag
      601 cagcagatca acggtgtgga gcacatgctc tacatctcgt cgaactcgac cgtcgacggc
      661 cgcttctcca tctcggtcac cttcgacctc ggcaccgacc tcgacaccgc gcaggtgcag
      721 gtgcagaacc gcgtggccat cgcccagccg cgtttgcccg ccgacgtgcg caacatcggc
      781 gtcacggtcg ccaaggcgtc gcccgacctg atgatggtcg tgcacttgta ctcgcccgac
      841 cagtcgcgcg acacgctgtt catctccaac tacgcgagcg tcaacgtcgt cgacgtgttg
      901 agccgcatcc agggcgtcgg ctcgatcacc gtattcggca gccgcgacta ctcgatgcgc
      961 gtgtggctgg atccggatcg cttgcagtcg ctcgggctca ccgccaacga cgtcacgacg
     1021 gcgctgcagc ggcagaacgt gcaggtcgcc gccggcatct tgaatcagcc gccggtgaag
     1081 cagccgggcg cgttccagat ctcggtgcag acgcaggggc gtctcgccga tcccaatgag
     1141 ttttcgcaga tcgtcgtcaa acagagcggc gatgcggtgg tgcgtctcaa ggacgtggcg
     1201 cgcgtcgagc tcgccgccct cgactacggc gtgaactcct acctcgacaa ataccccgcg
     1261 accggccttg gcgtcttcca gcttcccggt tcgaacgcca tcgagacggc ggagaagatc
     1321 aagtccacca tggaggaact ctccaagagc ttccccaagg gactgaagta cgacatcatc
     1381 tacaacccga ccgacttcat ccagcagtcg gtcgacgcgg tggtgaccac catcctcgaa
     1441 gccgtcgtgc tcgtcgttct ggtggtcatc ctgttcctgc agacgtggcg cgccgcgatc
     1501 atcccgatcg ttgccattcc ggtgtcgctc atcggcacgt tcttcctgat gtcgctgttt
     1561 ggcttctcgc tgaacaacct gtccctgttc gggctcgtgc tcgccatcgg tatcgtcgtc
     1621 gacgacgcga tcgtcgtggt ggagaacgtc gagcgcaaca tggcggcggg gatgagcccg
     1681 cgtgaagcgg cgtatcgcac catggaagag gtcggagccg cgctcgtcgc catcgcgctg
     1741 gtgctgtccg ccgtgttcgt gccgtcgacc ttcatcaccg gcatctccgg ccagttctac
     1801 cggcagttcg ccctcactat cgccggcgcc acggtcatct cgctggtcgt gtcgctgacg
     1861 ctgtcgccgg cgctatgcgc gctgctcctc aagccgcaca gcgagaagca caagacgccc
     1921 tggtatatgt ggccggtgga agcgttcttc cgcgccttca actggtcgtt cgaaaaggct
     1981 tcgcacggtt atgcgtggat ggtcggccgg ctcgtgcgcc tttcggtcct gatgctcata
     2041 gtctacgtcg gcatcatcat cttcggcatg aacgagttcc gcaagacacc ggttggcttc
     2101 atcccggcgg tcgacggcgg ctatctcatc accgtcacgc agctaccgcc tgccgcatcg
     2161 ctcgaccgca cggacgccgt caaccagcgc gtcgtcgaga tggcgctcga gacgccaggc
     2221 gtagcgcacg cggtgaatat cgtcggc
//
LOCUS       DASUGA010000692         2530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_209948, whole genome shotgun sequence.
ACCESSION   DASUGA010000692 DASUGA010000000
VERSION     DASUGA010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209948"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VFX21_13770"
     CDS             <1..820
                     /locus_tag="VFX21_13770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX5097088.1"
                     /translation="RVLMDIGGGDAGPGALQPTWEMDFDSWPPSDVEPTAFYLGPDGA
                     LTNDAPTADGEDEYTPDTSARNPTSLPEGDAWAAQPPYEWQPVMANDGLGYVTEPLTD
                     DLTIAGPASMDLFLKSSAADTDLQVTLSEVRPDGKESYVQFGVLRASYRAPDKDSNDL
                     EVVPTYLKADRAELPADEFTLVRVPIFPVAYVFRAGSRLRVTIQAPGGERPSWMFDTK
                     EDGSTTNTISRSPALASRLMLPVLPGRTADLPLPACGAPRGQPCRMYVAAGNGG"
     gene            complement(824..1672)
                     /locus_tag="VFX21_13775"
     CDS             complement(824..1672)
                     /locus_tag="VFX21_13775"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX5097089.1"
                     /translation="MSEALEVPRVPAGTAVGEIEAQIRAAGCCVVERLAPESLLQQFE
                     TELDPYLRATATGDDEFSGRNTRRTGSLIARAPAFRTLATDPTVLGVLDKILGDHATS
                     YQLHLTQVIEIGPGEPAQLVHRDQWAFDFFTFPAGFEVECHVMWALSEFTEANGATRV
                     IPGSHHWEDKLRPTPDQTVPAEMPRGSVLLYVGSLYHGGGANHTETPRRGINVGYTLS
                     WLRQEENQYLACPPEIARELPEDLVKLMGYQRGAYALGYYGDLRDPMEFLHGVHDRAP
                     SFGIRD"
     gene            1757..2077
                     /locus_tag="VFX21_13780"
     CDS             1757..2077
                     /locus_tag="VFX21_13780"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5097090.1"
                     /translation="MDAATTLRRARRAHGLSKRALARKAETSPAAVVMYENGSRVPTV
                     NTLLRLLEAAGWHVELRLSPRARKPDDATAARWLREVLDLADRLPQRPAARELAYPPF
                     GRTA"
     gene            2074..>2530
                     /locus_tag="VFX21_13785"
     CDS             2074..>2530
                     /locus_tag="VFX21_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097091.1"
                     /translation="MTPEDALPLADKVIALNAALARSDFDYAFGGALALAYHVEDPRG
                     TRDIDINIFVSADRAEEVFRSLPTEVIWDAADLRTTLRDGQVRLFWGDTPVDLFLSTH
                     EFHDEASLSTREVPFDGARIPILGATELTVFKAFFNRTQDWADIEKMVAV"
BASE COUNT          414 a          875 c          835 g          406 t
ORIGIN      
        1 gcgggtgttg atggacatcg ggggcggcga cgccggtccc ggcgcgctgc aaccgacctg
       61 ggagatggac ttcgacagct ggccgccgtc cgacgtcgaa cccacggcgt tctatctcgg
      121 gcccgacggc gcgttgacca acgacgcgcc gacggccgac ggcgaagacg agtacacgcc
      181 cgacacgtcc gcgcggaacc cgacgagcct cccggaaggc gacgcgtggg ccgcgcaacc
      241 gccgtacgaa tggcagccgg tcatggccaa cgacggcctc gggtatgtca ccgaaccgct
      301 caccgacgat ctcaccatcg cgggcccggc cagcatggac ctgttcctga agtcgagtgc
      361 ggccgacacc gacctgcagg tgaccctgtc ggaagtccgg cccgacggca aagagagcta
      421 cgtgcagttc ggcgtcttgc gcgcgtcgta ccgcgcaccc gacaaggact ccaacgacct
      481 cgaagtcgtg cccacctacc tgaaggccga tcgcgccgag cttcccgcgg acgagttcac
      541 gttggtacgc gtgccgatct tcccggtcgc ctacgtgttc cgcgccggtt cgcgactgcg
      601 cgtcacgatc caggcgcccg gcggtgaacg cccgtcgtgg atgttcgaca ccaaggaaga
      661 cgggtcgacg acgaacacga tctcgcgttc acccgcgctc gcatcgcgct tgatgctgcc
      721 ggtgttgccg ggtcgtactg cagatctccc gttgcccgcg tgcggtgcac cgcgcggtca
      781 gccgtgccgc atgtatgtcg cggccgggaa cggcggctga ccgtcagtcg cggataccga
      841 agctcggcgc gcggtcgtgc acgccgtgga ggaactccat ggggtcgcgc aggtcgccgt
      901 agtagccgag cgcgtacgcg ccgcgctggt agcccatgag cttgacgaga tcctccggga
      961 gctcgcgcgc gatctcgggc gggcacgcga ggtactggtt ctcctcttga cgcaaccacg
     1021 acagcgtgta cccgacgttg atgccgcggc gcggcgtctc ggtgtggttg gcgccgccgc
     1081 cgtggtacag cgatccgacg tagaggagca ctgacccgcg cggcatctcc gcaggaaccg
     1141 tttggtcggg cgtcgggcgg agcttgtctt cccagtggtg gctgccgggg atgacgcgcg
     1201 tcgcgccgtt cgcttcggtg aactccgaca gtgcccacat cacgtgacac tcgacctcga
     1261 aaccggccgg gaaggtgaag aagtcgaacg cccattggtc gcggtgcacg agctgcgcgg
     1321 gctcgccggg accgatctcg atcacttgcg tgaggtgcag ttggtagctc gtcgcatggt
     1381 cgccgaggat cttgtcgagc acgccgagca cggtcggatc cgtcgcgagc gtgcggaacg
     1441 ccggcgcgcg cgcgatgagg ctgccggtgc ggcgcgtgtt ccggccgctg aactcgtcgt
     1501 cgccggttgc cgtcgcgcgc agatagggat cgagctcggt ctcgaactgc tgcagcaacg
     1561 actccggcgc gagccgttcg acgacgcagc acccggcggc gcggatctgc gcctcgatct
     1621 cgccgaccgc cgtgccggcc ggaacccgcg gaacctcgag tgcctcggac atgctcggat
     1681 tctgccccag gaccattccc gccaaatgtt atcggagtcg ataccatctc gctatcgttt
     1741 cagataacat gtggacatgg atgctgccac gacactgcgc cgagcccgcc gagcccacgg
     1801 tctgtccaag cgcgccctgg cccggaaggc ggagacgtct cccgccgccg tggtgatgta
     1861 cgaaaacggc agccgagtac cgacggtcaa caccctcctg cgactcctcg aggccgcggg
     1921 ctggcacgtc gagttgcggc tgtcaccgcg cgcacgaaag cccgacgacg cgacggccgc
     1981 gcgctggttg cgtgaggtgt tggatctcgc cgaccgcctg ccacagcgtc cggccgcacg
     2041 cgagcttgcc tacccgccgt tcggccggac ggcgtgacac cggaagacgc gcttccgctc
     2101 gcagacaagg tgatcgcact caacgccgcg ctcgcgcggt cggacttcga ctacgcattc
     2161 ggcggcgcgc tggcgctggc gtaccacgtc gaggatccgc gcggcactcg cgacatcgac
     2221 atcaacatct tcgtgtccgc cgaccgcgcc gaggaagtgt tccgatccct accaaccgaa
     2281 gtcatatggg acgcggccga ccttcgaacc acactgcgag acggtcaggt gcgcctcttt
     2341 tggggcgaca cgcccgtcga cttgtttctt tcgacacacg agttccacga cgaagcatca
     2401 ctctcgacac gcgaagttcc cttcgacggc gcgcgtatcc ccattctcgg cgcaaccgaa
     2461 ctcacggtct tcaaggcgtt cttcaaccgg acccaggact gggccgacat cgagaagatg
     2521 gtcgccgtcg
//
LOCUS       DASUGA010000693         2518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_384279, whole genome shotgun sequence.
ACCESSION   DASUGA010000693 DASUGA010000000
VERSION     DASUGA010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384279"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..>2518)
                     /locus_tag="VFX21_13790"
     CDS             complement(61..>2518)
                     /locus_tag="VFX21_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX5097092.1"
                     /translation="DAKVIPALVARRVESGTDLVEAFRATVASFEGSVAIASQATANP
                     DLMLLAQRGSGQALYVGLAPDAFVVASEPYGLVAECTQYLRLDGETMLDPGNPASQGQ
                     VVILDRTRAGTLAGIRRLSYDGRELPVTDADLQTAQITTRDVDRGAAPHYLLKEISEA
                     PGSFRKTLRGKIVEQHDRLSVRLPDETLTAAVLDRLRSGAVRRVLVIGQGTAAIAGQS
                     LMHALRSALGGHPVNVEAITATELSGFGLASDMSDTLIVAISQSGTTTDTNRTVDLAR
                     SRGALVVAIVNRRQSDLVDKADGVLYTSDGRDVEMSVASTKAFYSQVAAGILLSFAIA
                     MELGVDDDAARTYRHERLVAVRELPAAMVEMIDRRAAIGAVAQRNALSRRSWAVVGNG
                     VNRVAAQEIRIKLSELCYKSIACDVTEDKKHIDLSAEPMILVCAAGLRGSNADDVAKE
                     CAIYRAHKAAPIAIVDDGETRFTAAIDTIAVPTVHPDFAFVLCAVAGHLFGYEAALAI
                     DASARPLREARGCVETALVASRAGQDPLEALAGEITPHAAQFFDELRTGNSDGCLEAA
                     TAVRVASVLRYATGVVPLDAYQIEFGKVGTPSTVVEDLTAALTKAIEELTRPIDAIKH
                     QAKTVTVGISRSDETLLVVRLVQEALAAGAPRDSLTYRALRTLVALDPAVESIAGWTR
                     YNIAGDTINVIDRGGIARDIPSRTDGNPVLRGTKHRAATEREVTVVRGRADGRTLILV
                     PEVKGNQTTGLTLLHVRFHDRLPGDAARRVLEGYRNRYQAIADQVTETEPVMRDDVLG
                     TIDLIDLLTVPVVQLADSWR"
BASE COUNT          397 a          839 c          854 g          428 t
ORIGIN      
        1 tgcgatcgcg cgccggtcgg cctcccccat ctcatccggg gtgaggaccg gctgcatcct
       61 tcagcgccag gagtcggcga gctggacgac cggcacggtc agcaggtcga tgagatcgat
      121 ggtgccgaga acgtcgtcgc gcatgaccgg ttcggtctcc gtcacttggt cggcgatggc
      181 ctggtaccga ttgcggtacc cctccaggac gcggcgtgcc gcgtcgccgg gcaagcggtc
      241 gtggaatcgc acgtgcagca gcgtcaggcc ggtcgtctgg ttgcccttca cctcgggaac
      301 gaggatgagc gtgcgcccgt cggcgcgtcc ccgcacgacc gtgacttcgc gctcggtcgc
      361 ggcgcggtgc ttcgtgccgc ggagcacggg gttgccgtcg gtgcggctcg gaatgtcgcg
      421 cgcaatgcca ccccggtcga tcacgttgat cgtgtcgccg gcgatgttgt agcgggtcca
      481 tcccgcgatc gattcgacgg ccggatcgag cgcgaccagc gtgcgcagcg cgcgataggt
      541 gaggctgtcg cggggtgcgc ccgccgcgag cgcctcctgc acgagtcgca cgacgagcag
      601 cgtctcgtcc gagcgggaga tgccgacggt cacggtcttg gcctgatgct tgatcgcgtc
      661 gatcggccgc gtcagttcct cgattgcttt cgtgagcgcg gcggtgaggt cttcgaccac
      721 cgtgctcggt gttccgacct tcccgaactc gatttggtag gcgtcgagcg gcacgacccc
      781 cgtcgcgtac cgcagaaccg atgcgacccg gactgcggtc gccgcttcca aacagccgtc
      841 ggagttgccg gttcgcagtt catcgaagaa ctgagcggcg tgcggcgtga tctcgcccgc
      901 caacgcttcg agaggatcct gtccggcacg cgatgcgacc agcgccgtct cgacgcaccc
      961 ccgcgcctcg cgcaacggtc gcgccgacgc gtcgattgcg agcgccgcct cgtagccgaa
     1021 caggtgaccc gcgaccgcgc agagcacgaa cgcgaagtcg ggatgcacgg tgggaaccgc
     1081 aatcgtgtcg atcgccgcgg tgaaccgtgt ttcaccgtcg tcgacgatcg cgatcggcgc
     1141 agctttgtga gcgcggtaga tcgcacactc cttggcaaca tcgtcggcgt tcgagcctcg
     1201 caggcccgcc gcacagacca ggatcatcgg ctccgccgac aggtcgatgt gcttcttgtc
     1261 ctcggtgacg tcgcaggcaa tcgacttgta gcaaagctca gagagtttga tgcgaatttc
     1321 ttgcgcggcg acacggttga cgccgttgcc gacgaccgcc cacgaccggc gactgagcgc
     1381 attgcgctgc gcaaccgcgc cgatcgccgc gcgccggtcg atcatctcca ccatggccgc
     1441 cggcagctcg cgtactgcga ccaagcgttc gtggcgatag gtgcgcgcgg cgtcgtcgtc
     1501 gaccccgagt tccatcgcga tcgcaaacga caacaagatg cccgccgcga cctgcgaata
     1561 gaacgccttg gtggatgcaa cgctcatctc gacgtcgcgc ccgtcggacg tgtagagcac
     1621 accgtcggcc ttgtcgacga ggtcgctctg gcgccggttc acgatggcaa ccacgagtgc
     1681 accgcggctg cgcgcgaggt caacggtccg gttcgtgtcg gttgtcgtgc ccgactgcga
     1741 aatcgcgacg attagcgtgt cgctcatgtc ggaggcgaga ccgaaccctg agagctcggt
     1801 ggcggtgatc gcttcgacgt tgaccggatg gccgcccaac gcactgcgca gcgcgtgcat
     1861 caagctctgc ccggcaatcg ccgcggtgcc ctggccgatc accaagacgc ggcggacggc
     1921 cccggaacgc agacggtcga gcaccgccgc cgtgagcgtt tcgtcgggga gacgaacaga
     1981 aagccgatcg tgctgctcga cgatcttgcc gcgcaatgtc ttgcggaagg agccgggcgc
     2041 ttccgaaatc tccttcagca ggtagtgcgg cgcggcaccg cgatcgacgt cgcgcgtcgt
     2101 gatctgcgcg gtttgcaggt cggcgtcagt gaccgggagt tcgcgcccgt cgtacgacaa
     2161 gcgccggatc cctgcgagcg ttcctgcgcg ggtgcggtcg agaatcacca cttggccctg
     2221 actcgctggg ttgcccgggt cgagcatcgt ctcaccgtcg aggcgcaagt actgcgtgca
     2281 ctcggccacc aagccgtagg gctcgctggc aacaacgaac gcgtcgggcg cgaggccgac
     2341 gtacagggcc tgtccactgc cgcgttgcgc aagcagcatg aggtcggggt tcgcggttgc
     2401 ttgcgacgca atcgcgaccg agccttcgaa ggatgccacc gtggcgcgga atgcctcgac
     2461 aagatcggtt ccggattcca cgcgccgcgc caccaacgcg ggaatgacct tggcgtcg
//
LOCUS       DASUGA010000694         3512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_348598, whole genome shotgun sequence.
ACCESSION   DASUGA010000694 DASUGA010000000
VERSION     DASUGA010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348598"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1376
                     /locus_tag="VFX21_13795"
     CDS             <1..1376
                     /locus_tag="VFX21_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013424206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5097093.1"
                     /translation="LPDDAPSVAAYLQEHGYKTALLGKAHFEPGFDFDLKYAENFMSP
                     RGLVGPYRGFQHAELAMHVPTVGKRTLQHYGQWLVDNHGLEAAQGFSPLLSAEPGGET
                     GAPETRINPIPRDWYHTDWVADRTISYLDSLSDDDDWFVWMSFPDPHHPWDPPASELH
                     RCNWRDLDLPPGHPGSPEAIERVLAQKPEHWLAMWEGRWINGEGGPGTYRPQNVSDDN
                     IREVNAMCHVMNELIDEACGRVMRTIAARGWDADTDVIFTTDHGELQGDYGLIFKGPF
                     HTDSLMRLPMVWRPAPSAGVTPAEIEEPVGQVDLAPTFCSIAGVPVPDWMQGAPLPTA
                     PGSGRERVLCEWDSQFPGYGMHLRSIYRDGWLCTAYEKSTVGEPNGLEEFFAATGMFG
                     GGIGPRSSVSYTGSEGELYDVANDPHQWVNRWDDPDYKALRDDLVADLYDALPAERKV
                     LKVAAPA"
     gene            complement(1354..2184)
                     /locus_tag="VFX21_13800"
     CDS             complement(1354..2184)
                     /locus_tag="VFX21_13800"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX5097094.1"
                     /translation="MNMAARDLRTDTALTKDPNVPGRYRCDIDEGWKVMYVFGGVSMY
                     TALRAMEEALGRDDLQLVTANAIFLAPVPPGPVEIDVSVLRDGRRASQVAADLHVPGA
                     GPALRVHGVFGAAHDTDLAQQDVRYPEVPKPADAPMPPNDRPNPFGTINFHQQTEWRP
                     VTPLDQPGPGQFMSWVRLTEGDPHLLALAVHGDVLGPAVGRAIGPRDMPFMVLSLEIG
                     IRFVAAPVTSWVLQEIEAWHIGDGYATGPARLWDEDGNLCAIATQTANLRVIKPELRP
                     "
     gene            2267..3271
                     /locus_tag="VFX21_13805"
     CDS             2267..3271
                     /locus_tag="VFX21_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017739928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX5097095.1"
                     /translation="MTTNRKMLLAQRPVGVPTKDCFELVEDAVPDAEDGEAIVEVAYL
                     SIDPTIRGWLNDEEGYLPPIQLGEVVRSGIVGTVVASKTDMFPVGMKVTGLGGWQQYL
                     RIGGSTGALVMPLADDIELLDAVSVFGITGITAFVGIFDIGQVQAGDVFVVSGAAGGV
                     GSIAGQIAKILGCRVVGIAGTDEKCAWLVDDLGFDAAINYKTHDVPTALRDACPNGID
                     VYFDNVGGDILNAALDLMNLNGRIIACGMISQYNDTERPPGPSNIFRIVGQRLRMQGF
                     ILLDHIDRFGAATEQLSQWVKEGKLQNRVTIVDGLEEAPRALNMLFTGENIGKVAVRV
                     "
     gene            3264..>3512
                     /locus_tag="VFX21_13810"
     CDS             3264..>3512
                     /locus_tag="VFX21_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5097096.1"
                     /translation="MSEPRLQPLSSDAIDPELPAAFGGKVLNIFGTLAYHPKLMKRWL
                     VFGNHVLAKSTLPARDREILILRTGWKCRSPYEWGQHVV"
BASE COUNT          565 a         1182 c         1208 g          557 t
ORIGIN      
        1 cgctaccgga cgacgcgccg agtgtcgccg cgtatctcca ggagcacggg tacaagaccg
       61 cgctgctcgg caaggcgcac ttcgagccgg gcttcgactt cgacctgaag tacgccgaga
      121 acttcatgtc gccgcgcggc ctggtcggcc cgtatcgagg cttccaacac gcggagctcg
      181 cgatgcacgt gccgacggtg ggcaagcgga cgctgcagca ctacggccaa tggctcgtcg
      241 acaaccacgg tctggaggcc gcgcagggct tctcgccgct gctgtcggcc gagccgggcg
      301 gcgagaccgg cgcgccggag acgcgcatca acccgatccc gcgtgactgg taccacaccg
      361 actgggtcgc ggaccgcacg atctcgtacc tcgactcgct ctccgatgac gacgactggt
      421 tcgtgtggat gtcgttcccc gacccgcacc acccgtggga tccgcccgcg tcggagctgc
      481 accgctgcaa ttggcgcgac ctcgacctgc cgcccgggca tccgggttcg ccggaagcga
      541 tcgagcgcgt gctcgcacag aagccggagc actggctcgc gatgtgggaa gggcgctgga
      601 tcaacggcga aggcggcccg ggcacgtacc ggccgcagaa cgtgagcgac gacaacatcc
      661 gcgaagtcaa cgcgatgtgc cacgtcatga acgagctcat cgacgaagca tgcggtcgcg
      721 tcatgcgcac gatcgcggcg cgcggatggg acgccgacac cgacgtcatc ttcaccaccg
      781 accacggcga gctgcagggc gactacgggc tgatcttcaa gggcccgttc cacaccgact
      841 cgctcatgcg cctcccgatg gtgtggcggc ccgcgccgtc ggccggcgtc acgcccgccg
      901 agatcgagga gccggtcggc caggtcgacc tcgcgccgac gttctgctcg atcgcgggtg
      961 tgcccgtgcc cgactggatg caaggcgcgc cgctcccgac cgcgcccggt tccgggcgcg
     1021 aacgggtgtt gtgcgagtgg gactcgcagt tccccggcta cggcatgcac ctgcgttcga
     1081 tctaccgcga cggctggctc tgcacggcgt acgagaaatc gaccgtcggt gaaccgaacg
     1141 ggctcgaaga gttcttcgcg gcgaccggca tgttcggcgg tggcattggc ccgcgcagct
     1201 cggtctcgta caccggcagc gaaggcgagc tctacgacgt cgccaacgat ccgcaccagt
     1261 gggtgaaccg ctgggacgac cctgattaca aggcgttgcg tgacgacttg gttgccgacc
     1321 tctacgacgc gctgcccgcc gaacgcaagg tgctcaaggt cgcagctccg gcttgatcac
     1381 gcgcaggttc gcggtctgcg tcgcgatcgc gcacaggttc ccgtcttcgt cccagaggcg
     1441 ggcgggacct gtcgcgtaac cgtcgccgat gtgccacgcc tcgatctcct ggagcaccca
     1501 ggaggtcacg ggtgcggcga cgaaccggat gccgatctcg agcgacagca ccatgaacgg
     1561 catgtcgcgc gggccgatcg cgcgcccgac cgccggtccg agcacgtcgc cgtgcacggc
     1621 cagcgcgagt aggtgcgggt cgccctcggt gagccgcacc cacgacatga actgaccggg
     1681 gcccggttgg tcgagcggcg tgaccggtcg ccattcggtc tgctggtgga agttgatcgt
     1741 gccgaacggg ttgggccggt cgttgggcgg catcggtgcg tctgccggct tcgggacctc
     1801 ggggtagcgc acgtcttgct gtgcgagatc ggtgtcgtgc gcggcgccga acacgccgtg
     1861 cacccgcagc gccggtcccg caccgggcac atgaaggtcg gcggcgacct gtgacgcgcg
     1921 gcggccgtcg cgcaggacgc tgacgtcgat ctcgacgggg cccggcggca cgggcgcgag
     1981 gaagatcgcg ttggcggtga cgagctgcag gtcgtcgcgg ccaagcgctt cctccatcgc
     2041 gcgcagcgcc gtgtacatcg acacgccgcc gaacacgtac atgaccttcc agccctcgtc
     2101 gatgtcgcag cggtagcgac cggggacgtt cgggtccttg gtgagcgcgg tgtcggtgcg
     2161 caggtctctc gctgccatgt tcatggtcga ttccacgctg ctcgcgtgtc ggctcctcgg
     2221 tgcttcgtcg ctgtcatctc ccggccgtcg agcctacgct gcgcccgtga cgaccaatcg
     2281 aaagatgttg ctggcgcagc ggcccgtcgg tgtgccgacg aaggactgct tcgagctcgt
     2341 cgaagacgcc gtgcccgacg cggaggacgg cgaagcgatc gtcgaggtcg cgtacctctc
     2401 gatcgatccg acgatccgcg gctggctcaa cgacgaagag ggctacctcc cgccgatcca
     2461 actcggcgag gtggtgcgca gcgggatcgt cggcaccgtc gtcgcgtcga agaccgacat
     2521 gttcccggtc gggatgaagg tgaccggcct cggcgggtgg cagcagtacc tgcgcatcgg
     2581 cggcagtacc ggtgcgctcg tgatgccgct cgccgacgac atcgaattgc tggacgcggt
     2641 cagcgtgttc ggcatcaccg gtatcaccgc gttcgtcggc atcttcgaca tcgggcaggt
     2701 gcaagccggc gacgtgttcg tcgtgtccgg cgcggcgggc ggtgtcggca gcatcgcggg
     2761 acagatcgcg aagatcctcg gctgtcgcgt cgtcggaatt gcgggcaccg acgagaagtg
     2821 cgcgtggttg gtcgacgacc tcgggttcga cgccgcgatc aactacaaga cccacgacgt
     2881 gcccacggca ctgcgcgacg cgtgcccgaa cgggatcgac gtctacttcg acaacgtcgg
     2941 cggcgacatc ctgaacgcgg cgctcgacct gatgaacctg aacgggcgca tcatcgcgtg
     3001 cgggatgatc tcgcagtaca acgacacgga gcgcccgccg ggcccgagca acatcttccg
     3061 catcgtcggg caacgactcc ggatgcaggg tttcatcctc ctcgaccaca tcgaccgctt
     3121 cggcgccgcg accgagcagc tgtcgcagtg ggtgaaggag ggcaagctgc agaaccgcgt
     3181 gacaatcgtc gacgggttgg aagaggcgcc gcgcgcgctc aacatgttgt tcacgggcga
     3241 gaacatcgga aaggtggccg tccgtgtctg aaccgcgctt gcagccactg tcgtcggatg
     3301 cgatcgaccc cgagctgccg gccgcgttcg gcggcaaggt cctcaacatc ttcggcacgc
     3361 tcgcgtacca cccgaagctg atgaagcgct ggctcgtctt cggcaaccac gtgctcgcga
     3421 agtcgacgct gccggcgcgc gaccgcgaga tcctgatcct gcgcaccggc tggaaatgcc
     3481 gctcgccgta cgaatggggt cagcacgtcg tg
//
LOCUS       DASUGA010000695         2021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1217578, whole genome shotgun sequence.
ACCESSION   DASUGA010000695 DASUGA010000000
VERSION     DASUGA010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217578"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="VFX21_13815"
     CDS             complement(<1..146)
                     /locus_tag="VFX21_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015888670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX5097097.1"
                     /translation="MSARVGVVLFPGSNCEFDVIEAVRRLGGEAEILWHGDDQLRGVD
                     AVVVP"
     gene            complement(143..418)
                     /gene="purS"
                     /locus_tag="VFX21_13820"
     CDS             complement(143..418)
                     /gene="purS"
                     /locus_tag="VFX21_13820"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF004630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX5097098.1"
                     /translation="MQFSVQVEVQQRPGIADPEGSTIERSLPTLGFDGIRDVRVGKSI
                     RFLIDAADEASARAEVDDLCQRFLTNPVIEDAVVHVEPAAVDSVGDT"
     gene            complement(423..1175)
                     /locus_tag="VFX21_13825"
     CDS             complement(423..1175)
                     /locus_tag="VFX21_13825"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEX5097099.1"
                     /translation="MTIDFAWGRPGVYALVESGCRTIVVVDVLRFTTAVDVGVSRGAA
                     IYPYRWHDGTEHAYAKSHDAELAVKGFDVDNDHPWSLSPVGLARIPAGTKLVLPSPNG
                     ATIAFEAAERGDVEVVAGCLRNARAVASFLRARPDRVGVVASGEQWKPDEALRPAVED
                     LLGAGAIIHALDPEGRDVTPDAGAAAGAFVAMTERLEWALLECPSGRELRGHGWDADV
                     RAAAEHGVSECVPVLVDGCFVDGSGAAARMGE"
     gene            complement(1172..>2021)
                     /locus_tag="VFX21_13830"
     CDS             complement(1172..>2021)
                     /locus_tag="VFX21_13830"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX5097100.1"
                     /translation="GKVRDIYDAGDGRLLLVASDRISAFDVVMDEPVPDKGRVLTAMS
                     AFWFERFADVVGNHLISTRLEDLPPAAQRPEWEGRVMLCRRAEMLPVECIVRGYITGS
                     AWKEYRAEGTMHGTPLPEGLLESSKLPEPVFTPSTKAADGDHDVNISFDDAVSLIGRE
                     TAERARDVSLELYTKGAAWAAERGIIIADTKFELGVIDGELVLADEVLTPDSSRFWPA
                     DQWKPGSTPPSFDKQPVRDYLDTLDWDKKPPPPPLPPEVIEASRARYIEAYERVTGRS
                     FADWHG"
BASE COUNT          336 a          730 c          653 g          302 t
ORIGIN      
        1 ggcacgacca ccgcgtcgac gccgcgcaac tggtcgtcgc cgtgccagag gatctcggct
       61 tcaccgccca gccgtcgtac ggcttcgatg acgtcgaact cgcagttcga gcccgggaac
      121 aggacgactc cgacgcgcgc gctcatgtgt ccccaacgct atcgaccgca gccggctcga
      181 cgtgcacgac ggcgtcttcg atcaccggat tcgtcaggaa ccgttggcac aggtcgtcga
      241 cctccgcgcg ggccgacgct tcgtcggccg cgtcgatgag gaagcggatg ctcttgccga
      301 cgcgcacgtc gcggatcccg tcgaacccga gggtgggcaa cgaccgctcg atggtggagc
      361 cttccgggtc ggcgatcccg ggccgctgct gcacctcgac ctgcaccgag aactgcatgg
      421 cgtcactctc ccatacgagc ggcggcaccc gaaccatcga cgaagcagcc gtccacgaga
      481 accggcacgc actcgctcac accgtgctcg gccgcggcgc gcacgtcggc gtcccacccg
      541 tgcccccgca attcgcgccc ggatgggcat tcgagcaacg cccactcgag acgctccgtc
      601 atggcaacga acgcaccggc cgccgcgccg gcgtccggcg tgacgtcgcg accttccggg
      661 tcgagcgcgt ggatgatcgc accggcaccc aacaggtcct cgacggccgg tcgaagtgcc
      721 tcatcgggct tccactgctc gccggaggcg accacgccga cgcggtcggg tcgcgctcgg
      781 aggaacgacg cgaccgcccg cgcgttccgc aagcacccgg caacgacctc gacgtcgccg
      841 cgctctgccg cttcgaacgc gatcgtcgcc ccgttcggtg acggcagcac gagcttggtt
      901 ccggcaggta tgcgagcgag tcccaccggt gacagcgacc acggatggtc gttgtcgacg
      961 tcgaagccct tcaccgcgag ctcggcgtcg tgcgacttcg catacgcgtg ctcggtgccg
     1021 tcgtgccagc gatacggata gatcgccgcg ccccggctga cgccgacatc gaccgcggtg
     1081 gtgaaccgaa ggacgtcgac caccacgatc gtgcggcaac ccgactcgac cagcgcgtag
     1141 acgccgggtc gtccccacgc gaagtcgatc gtcacccgtg ccagtcggcg aacgaccgac
     1201 cggtgacgcg ctcgtacgcc tcgatgtagc gagcacgcga agcctcgatg acctcaggcg
     1261 gaagcggcgg aggtggcggc ttcttgtccc agtcgagcgt gtcgaggtag tcgcgcacgg
     1321 gctgcttgtc gaacgacgga ggagtcgacc cgggcttcca ctggtcggcg ggccagaacc
     1381 gtgacgagtc gggggtgagc acctcgtcgg cgagcaccaa ctcgccgtcg atcacgccca
     1441 gctcgaactt ggtgtcggca atgatgatgc cccgttccgc cgcccaggcc gcgcccttcg
     1501 tgtagagctc gagcgagacg tcacgcgccc gttcggccgt ctcgcgcccg atcaatgaca
     1561 ccgcgtcgtc gaacgagatg ttgacgtcgt ggtcaccgtc ggccgctttg gtcgacgggg
     1621 tgaaaaccgg ctcgggcagc ttcgacgact cgagcagtcc ctccggcagc ggggtgccgt
     1681 gcatcgtgcc ctcggcgcgg tactccttcc acgccgaacc ggtgatgtag ccgcggacga
     1741 tgcactcgac gggaagcatc tcggcccgcc ggcacagcat cacgcgtcct tcccactccg
     1801 gccgttgcgc ggcgggtgga agatcctcga ggcgagtgga gatcagatgg ttaccaacca
     1861 cgtcggcaaa ccgctcgaac cagaacgccg acatcgcggt gagcacccgt cccttgtcgg
     1921 gcacgggttc gtccatcacc acgtcgaacg ctgagatgcg atcggacgcc accaacagca
     1981 gccgaccgtc accggcgtcg tagatgtcgc gcacctttcc c
//
LOCUS       DASUGA010000696         2366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1702251, whole genome shotgun sequence.
ACCESSION   DASUGA010000696 DASUGA010000000
VERSION     DASUGA010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702251"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VFX21_13835"
     CDS             complement(<1..450)
                     /locus_tag="VFX21_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-binding domain-containing protein"
                     /protein_id="HEX5097101.1"
                     /translation="MTDFPILTTLLLLPAVGAVLVMFAPKGRTDVIRVLGYATTIATL
                     GFAIFLLKEFDKADAGVQFVSHHQWMPDLGVSYSVGVDGISLFMVALTALLFPIGLLA
                     SANEKNLKSFVVWMLLLEAALIGVFIATDLVLFFVFFEVVLVPMYFLI"
     gene            complement(447..>2366)
                     /gene="nuoL"
                     /locus_tag="VFX21_13840"
     CDS             complement(447..>2366)
                     /gene="nuoL"
                     /locus_tag="VFX21_13840"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX5097102.1"
                     /translation="FATAIMTLTFLWSVLTFSALFSLDAEERINLSDGFTWISAGALH
                     VDFRFLVDPLSATMILFITGVGALIHLYSIGYMHGDPRFSRFFAYLNLFAASMLVLVL
                     GSSFVLTYLGWEGVALCSYLLVAFWFERNAPAVAGKKAFVTNRVGDVGFLLGMFLLFS
                     KVGTLDYFREGEAGIRLFGQVDFGGLSNTTVTAIALLLFVGAIGKSAQVPLHIWLPDA
                     MEGPTPVSALIHAATMVTAGVYLVCRANPFFEISDHAGTVVAWIGVITALFAATAAVV
                     QNDIKRVLAYSTISQLGYMFLAAGVGAYSAAMFHMITHAFFKALLFLAAGSVIHGLHD
                     EQDMRRMGGLRKWMPVTSGVFIVGWLAIAGIFPFAGFWSKDEILGAVWLRDDYALWFV
                     GAVTALLTAFYMTRQVWLVFYADERWRETHTTEHEIESAVVPAEESVDAHADPHGSGA
                     PRESPWTMTGPLVVLAGLATFGGLINLPFLKSDLDFLTLWLEPVFHEVPEVVPNSFAQ
                     GFILSTVALTVAFVGILLGRAFYKNGLRADGTDPVEQRLGGFSKVLENAYYLDVGVAK
                     FVSGPATKFAEFLTNGVDRTVIDGAVDGIGRGFSGSGSGLRKLQTGLVRNYALGISLG
                     AVLLIGYVAVRTVTT"
BASE COUNT          500 a          761 c          774 g          331 t
ORIGIN      
        1 gatgaggaag tacatcggga cgagcacgac ttcgaagaac acgaagaaca ggacgaggtc
       61 ggtcgcgatg aacacaccga tcaaggccgc ttcaagcagc agcatccaga cgacgaacga
      121 cttcaggttc ttctcattgg ctgacgcgag caagccgatc gggaacagca acgcggtcaa
      181 tgcgaccatg aacaggctga tgccgtcgac gccgacgctg tagctgacgc cgaggtcggg
      241 catccattgg tgatgcgaca cgaactggac gcccgcgtcg gccttgtcga actccttcag
      301 caggaagatt gcgaagccca gcgtcgcgat cgtcgtcgcg tagccgagca cgcggatgac
      361 gtccgtgcgc cccttcggcg cgaacatcac caggaccgcg ccgacggccg gaagcaagag
      421 caacgtcgtc aggatcggga agtccgtcac gtcgtcaccg tccgcaccgc gacgtaaccg
      481 atgagcagca ccgcgccgag gctgatgccg agcgcgtaat tgcgcacgag cccggtctgg
      541 agcttgcgca agccgctacc gcttccgctg aagcccctgc cgatgccgtc gaccgcgccg
      601 tcgatcacgg tgcggtcgac accgttggtc aagaactccg cgaatttcgt cgcgggaccg
      661 ctgacgaact tggcgacgcc gacgtcgagg tagtacgcgt tctcgagcac cttcgagaag
      721 ccaccgaggc gctgctccac cggatcggtg ccgtcggctc tcaggccgtt cttgtagaac
      781 gcgcgcccga gcaggatgcc gacgaacgcg acggtcagcg cgacagtcga gaggatgaag
      841 ccctgcgcga aggagttggg gacgacctcg ggcacctcgt ggaagaccgg ctccaaccag
      901 agggtcaaga agtcgagatc ggacttgagg aacggcaggt tgatgagacc gccgaaggtc
      961 gcgagacccg cgagcacgac aagcggcccg gtcatcgtcc acggcgactc gcgcggcgcg
     1021 ccggacccgt gcgggtcggc gtgcgcgtcg acggactcct cggcgggcac gaccgcggat
     1081 tcgatctcgt gctcggtcgt gtgcgtctca cgccagcgct cgtcggcgta gaagacgagc
     1141 cagacctggc gggtcatgta gaacgcggtc aatagcgccg tgaccgcacc gacgaaccac
     1201 aacgcgtagt cgtcacgtaa ccagacggca ccgaggatct cgtccttcga ccagaatccc
     1261 gcgaacggga agatgcccgc gatcgcgagc cagccgacga tgaacacgcc ggatgtgacc
     1321 ggcatccact tgcgcaagcc acccatgcgg cgcatgtctt gctcgtcgtg caggccgtgg
     1381 atcaccgagc cggcagcgag gaagagcagc gccttgaaga acgcgtgcgt gatcatgtgg
     1441 aacatcgccg cggagtacgc gccgacgccg gccgcgagga acatgtagcc gagctggctg
     1501 atcgtcgagt acgcgagcac gcgcttgatg tcgttctgga cgacggccgc cgtcgccgcg
     1561 aacagcgcgg tgatcacacc gatccacgcg accaccgtgc ccgcatggtc ggagatctcg
     1621 aagaacgggt tggcgcggca cacgaggtag acaccggcgg tcaccatcgt cgccgcgtgg
     1681 atcagcgccg agaccggcgt ggggccttcc atcgcgtcgg ggagccagat gtgcagcggc
     1741 acctgggcgc tcttgccgat cgcaccgacg aacagcaaca acgcgatcgc cgtcacggtc
     1801 gtgttcgaca agccgccgaa atcgacttgc ccgaacaggc gaatgcctgc ttcgccctcg
     1861 cggaagtagt cgagcgtgcc gaccttcgag aagagcaaga acatccccag caagaagccg
     1921 acgtcaccga cccggttggt gacgaacgcc ttcttgcccg cgaccgcggg cgcattgcgc
     1981 tcgaaccaga acgcgaccag caggtaggag cacagcgcga cgccttccca gcccaggtac
     2041 gtgagcacga agctcgagcc gagcacgagc acgagcatcg acgccgcgaa cagattgagg
     2101 tacgcgaaga accggctgaa acgcgggtct ccgtgcatgt agccgatcga gtacaggtgg
     2161 atcagcgcgc cgaccccggt gatgaacagg atcatcgtcg ccgacaacgg gtcgacgagg
     2221 aagcggaagt cgacgtgcag cgcgcccgcg ctgatccacg tgaacccgtc gctcagattg
     2281 atgcgttcct cggcgtcgag cgagaacagt gcgctgaacg tcagcaccga ccacaggaac
     2341 gtcagcgtca tgatcgcggt tgcgaa
//
LOCUS       DASUGA010000697         3436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_940401, whole genome shotgun sequence.
ACCESSION   DASUGA010000697 DASUGA010000000
VERSION     DASUGA010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940401"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1392)
                     /locus_tag="VFX21_13845"
     CDS             complement(88..1392)
                     /locus_tag="VFX21_13845"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEX5097103.1"
                     /translation="MPAFLGIPYATAERFARPVGVAYAPGSYTSFGPAAPQGRDPFES
                     IPGTPVGATSEDCLSCNVWAPEHADGLPVLFWVHGGSFIIGSSAQPVYDGARLAREQN
                     VVVVSCNYRIGALGFLDTRSIGGDAVNLGLHDVLAALAWVQANIGAFGGDPARVTVFG
                     ESAGGGVVAHLLASARSRGLFRSAIVQSGITDRTLGAATAAVVTDAVCTELGTRDLDV
                     MRTLPVDAIVAAQQAALPALLKPVGMMPFHPTVDDDLLHAPPAAALAGGVAAGVPLVA
                     GTTSDEMRLFVDPAAAAPERERLLKRVARYIGVSEADAVWPALFSDVEMQVPLRRVLD
                     AHAAHAPVYTYLFTWEAPGVGAAHGVDIPFTFGNFSDGWEKFVGADDAAERLSREMRD
                     AWAAFARDGEPGWSPYPSAYVFGRESSRPVDAHPLFARLPAL"
     gene            1412..2572
                     /locus_tag="VFX21_13850"
     CDS             1412..2572
                     /locus_tag="VFX21_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097104.1"
                     /translation="MVQGDVLGETENWFIRRGLPHFIAHYSASRDIWTRALPALTLLL
                     VAEIAINAPNRDFSVLTDIVVIAVAFGLLLGGWALINRQRGRRPFARPDDVGPFEIAA
                     FVVIPALVPLLAGSQVRDAVITAVFNVFLLVAIYFSTSYGLVAMTRWGLGRPVQQLEA
                     VMSLLARALPLLALLVTFLFLTAEVWQTAGAVEGAPYWIVVMLFPLVGVLFLVSRLPR
                     DVGELNHFEAGEIVPLVAGTPIETAAAECATRDAPPLGRREWGNVGLVALFSQGVQIF
                     IVSLLIGVFFVVLGALLVSEQTTESWAHQVHVLAHWSVGNSELVVTEELLRVAGSLTA
                     FSGLNFTVVLVTDVNYRQEFRAEVVAELRQAFAVRAVYLDSVSRSSATVSST"
     gene            2622..>3436
                     /locus_tag="VFX21_13855"
     CDS             2622..>3436
                     /locus_tag="VFX21_13855"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEX5097105.1"
                     /translation="MPGAVIVGMQWGDEGKGRFTDYLAKEALMCVRYQGGHNAGHTIV
                     VDDEVFKLQLVPSGVLYPWVTPMIGNGVVVDPKVLFEELDMLQTKNVDTSLLRVSGNA
                     HLIMPYHQELDRVTERYLGKSKLGTTKRGIGPAYGDKSWRVGLRVQDLLDPKIFREKL
                     DLALREKNGVLAKVYNRLPLDAEEIATEYLAMAPRLEPMIADTVHLVHEALDAGEQVL
                     FEGAQATYLDLDHGTYPFVTSSNPIAGGVCTGAGVGPRAITRVIGVAKAYVTRV"
BASE COUNT          521 a         1209 c         1174 g          532 t
ORIGIN      
        1 ctctacgagg ccgtccggcg cgacgcactc gccgaggtca ccgacaccgt gcagagggtg
       61 ctcggtcgcg cgcccgtccg cgcgttccta tagtgccggc aaccgcgcga acaacgggtg
      121 cgcgtccacc gggcgcgacg actcgcgtcc gaacacgtac gcgctcgggt acggcgacca
      181 tcccggttcg ccgtcgcgcg cgaaggccgc ccacgcgtca cgcatctcgc gcgagaggcg
      241 ttcggcagcg tcgtcggcgc ccacgaactt ctcccagccg tcggagaagt tgccgaacgt
      301 gaacgggatg tcgacgccgt gggccgcgcc gacgcccggc gcctcccacg tgaacaggta
      361 cgtgtagacg ggtgcgtgcg cggcgtgcgc gtcgagcacg cgccgcagcg gcacctgcat
      421 ctcgacgtcg ctgaagagcg cgggccagac cgcgtccgcc tcggacacgc cgatgtaccg
      481 agcgacccgc ttgagcaagc gttcccgttc cggggccgcc gcggcggggt cgacgaacag
      541 ccgcatctcg tcgctggtcg tgcccgcgac gagcgggaca ccggccgcga caccgccggc
      601 cagcgcggcc gcgggcggcg cgtgcagcag gtcgtcgtcg accgtgggat ggaacggcat
      661 catgccgacc ggcttcaaca gcgcgggcag cgccgcctgc tgcgcggcga cgatcgcgtc
      721 gaccggcagc gtgcgcatca cgtcgagatc gcgggtgccg agctcggtgc agacggcatc
      781 ggtgacgacc gccgcggtcg cggcgccgag ggtgcggtcg gtgatgccgc tctgcacgat
      841 cgcgcttcgg aacagcccgc gcgaccgcgc cgacgcgagg agatgcgcga cgacaccgcc
      901 accggccgac tcgccgaaca ccgtgacgcg cgccgggtca ccaccgaacg ccccgatgtt
      961 ggcctggacc caggccagcg ccgcgagcac gtcgtgcaag ccgaggttga ccgcgtcgcc
     1021 gccgatcgat cgcgtgtcga ggaagcccag cgcgccgatg cggtagttgc acgacacgac
     1081 gacgacgttc tgctcgcgcg cgagccgcgc accgtcgtac accggttgcg ccgacgaccc
     1141 gatgatgaac gaccctccgt gcacccagaa caagaccggg agcccgtccg cgtgctcggg
     1201 cgcccagacg ttgcacgaca ggcagtcctc gctcgtcgcg ccgactggtg tgcccgggat
     1261 cgactcgaac ggatcgcgcc cctgcggcgc ggccggcccg aacgacgtgt aggagccggg
     1321 cgcatacgcg acgccgaccg gccgcgcgaa ccgctcggcg gtcgcgtacg gaatccccag
     1381 gaatgcaggc accgacggcc agactaggcc ggtggtgcag ggcgacgtcc tcggcgagac
     1441 cgagaactgg ttcatccggc gcgggctgcc gcacttcatc gcgcactact cggcgagtcg
     1501 cgacatctgg acgcgcgcgc tccccgcgct gacattgctc ctcgtcgcgg agatcgcgat
     1561 caacgcgccg aaccgcgatt tctcggtgct gaccgacatc gtcgtgatcg cggtcgcgtt
     1621 cggtctgctg ctcggcggat gggcgctgat caaccggcag cgcggccgtc ggccgttcgc
     1681 gcgccccgac gacgtcggcc cgttcgagat cgcggcgttc gtcgtcatcc ccgcgctcgt
     1741 gccgctactc gcaggctcgc aagtgcgcga cgcggtcatc accgcggtgt tcaacgtctt
     1801 cctgctcgtc gcgatctact tctcgacctc gtacggcctc gtcgcgatga cccgttgggg
     1861 gctcgggcga cccgtgcagc agctcgaagc ggtcatgtcg ctgctcgctc gcgccctgcc
     1921 gctcctcgca ctgctggtga cgttcctctt cctgaccgcc gaggtgtggc agaccgcggg
     1981 cgcggtcgag ggcgcgccgt attggatcgt ggtcatgctc tttccgctcg tcggcgtgtt
     2041 gttcctcgtg tcgcgcctcc cgcgcgacgt cggcgagctg aaccacttcg aggccggtga
     2101 gatcgtcccg ctcgtcgccg gcacaccgat cgagaccgcg gcggccgagt gcgcgacccg
     2161 agacgcgccg cccctgggcc ggcgcgaatg gggcaacgtc ggtctggtcg cgctgttcag
     2221 ccagggtgtg cagatcttca tcgtgagcct gttgatcggc gtgttcttcg tggtgctggg
     2281 cgcgctgctc gtctcggagc aaacgaccga gtcgtgggcg catcaggtcc acgtgctcgc
     2341 gcactggtcg gtcggcaaca gcgagctcgt cgtcaccgag gaactgctgc gcgtcgccgg
     2401 ctctctgaca gctttctccg gtctcaactt caccgtggtc ctcgtgaccg acgtgaacta
     2461 ccgccaagaa ttccgtgccg aagtcgtggc cgagttgcgc caggcgttcg ccgtgcgggc
     2521 cgtctacctg gactccgtgt cccggtcgtc ggctacagtt tcctcaacgt gaccaccttt
     2581 cagaggcgca cgttgcacgc cacatcgccc gggggctcga catgccgggc gcagtgatcg
     2641 tcggcatgca gtggggcgac gaaggcaagg gccgcttcac cgactatctc gccaaagaag
     2701 cgttgatgtg cgtccgttac cagggcggcc acaacgccgg gcacacgatc gtcgtcgacg
     2761 acgaagtgtt caaactccag ctcgtcccga gtggcgtgct gtacccgtgg gtgacaccga
     2821 tgatcggcaa cggcgtcgtc gtcgacccga aggtgctctt cgaggagctc gacatgctcc
     2881 agacgaagaa cgtcgacacg agcttgttgc gcgtctcggg caacgcgcac ctgatcatgc
     2941 cgtaccacca agagctcgac cgcgtgaccg agcggtatct cggcaagagc aagctgggca
     3001 cgaccaagcg gggcatcggt ccggcatacg gcgacaagtc gtggcgcgtg gggctgcggg
     3061 tgcaagacct gctcgacccg aagatcttcc gcgagaagct cgatctcgcg ctgcgcgaga
     3121 agaacggcgt gctcgcgaag gtctacaacc ggcttccgct cgatgccgag gagatcgcca
     3181 ccgagtactt ggcgatggcg ccgcgtctgg aaccgatgat cgccgacacc gtgcacctcg
     3241 tgcacgaggc cctcgacgcg ggcgagcagg tcctcttcga gggcgcccag gcgacgtacc
     3301 tcgacctcga ccacggcacg tatccgttcg tcacgtcgtc gaacccgatc gcgggtggtg
     3361 tgtgcacggg cgccggcgtg ggaccacgtg cgatcacccg ggtgatcggc gtggccaagg
     3421 cctacgtcac gcgcgt
//
LOCUS       DASUGA010000698         2282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_904255, whole genome shotgun sequence.
ACCESSION   DASUGA010000698 DASUGA010000000
VERSION     DASUGA010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904255"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VFX21_13860"
     CDS             complement(<1..364)
                     /locus_tag="VFX21_13860"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5097106.1"
                     /translation="MWIPELPYEPSIPVVVRRAAEEFGDNDYVVLPDQRISFRDAERA
                     SRRLAKELLAAGAGKGTRVGIHIANGPEWVVAFLAATRIGALAMPYSTLYRPAELRRA
                     LRIGDVDVLLTTSTILGKD"
     gene            complement(394..1620)
                     /locus_tag="VFX21_13865"
     CDS             complement(394..1620)
                     /locus_tag="VFX21_13865"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5097107.1"
                     /translation="MPTDPKDVDPEYFRDRARVWLADHAPRRLTVADQQDPEYWSRAR
                     SFQRMQHAAGFAGIAWDERYGGQGLSLAHQMVFNDEAAGYELPSDAFQITLAILGMTI
                     LEHGSEEQKEGLLPEMLRGESVWVQLLSEPGAGSDLAAARMRAERDGDEWILNGEKVW
                     SSFAQWSDHAMVLARTDWDVPKHAGLTMFVMRLDTPGVTLLPLRQMSGDEEFCQEFFD
                     DVRLGADAVLGKVNGGWPVASAMFKYSRLMTSGAGLTGPTFAKDRGGDPDPGRELIDL
                     VNASERGSDPHVRQLIAHVVVDNVVSGLLANRIAANPDPNAGNIAKMFGALVLQRRCE
                     IELELRGESFIGWAPEDAPGARPLFEYLKGRTASVAGGTHEVMKNNVGERLLGLPREP
                     QLDKDLPFRAVIRDAH"
     gene            complement(1620..>2282)
                     /locus_tag="VFX21_13870"
     CDS             complement(1620..>2282)
                     /locus_tag="VFX21_13870"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX5097108.1"
                     /translation="WVQDAQNADILLVTCRDERGRVVQLLVSVDTPGVMIVPLETMDL
                     GRRLARVHFDEVQVPFDAVVGAPSDASAVDVQLRLAVVLQCAESVGATGRMLERSVEY
                     AKERVAFGRPIGSFQAVKHHLAEALMLVEGAQAATWAAARAVAADASDAARAVHVAKV
                     FVGTECPRVVEMCMQVHGGIAMTWEDDAHLYLRRVQSNRMLFGSPTWHADRLCDVVGV
                     AA"
BASE COUNT          376 a          785 c          764 g          357 t
ORIGIN      
        1 ggtcctttcc gagaatcgtc gatgtggtca ggaggacgtc gacgtcgccg atccgcagcg
       61 cgcgccggag ctccgcgggc cggtacagcg tgctgtaggg catggccaac gcaccgatgc
      121 gcgtggcggc aaggaatgcc accacccatt cgggtccgtt ggcgatgtga atgccgacgc
      181 gggtgccctt gccggcgccc gcggcgagca gctccttggc gagtcggcgg gaggcgcgct
      241 cggcgtcgcg gaacgagatt cgctggtcgg ggagcacgac gtagtcgttg tcgccgaact
      301 cttccgcggc acgacgtacg acgacgggga tcgacggctc gtacggaagc tcagggatcc
      361 acatgacagc gtcctcctct tcggccgcgc cggtcagtga gcatcgcgga tcaccgcgcg
      421 gaacggcagg tctttgtcga gctgcggctc acgaggcagt ccgagcaacc gctcaccgac
      481 gttgttcttc atcacctcgt gggtgccacc ggccaccgaa gcggtccgac ccttcaagta
      541 ctcgaagagg ggacgtgcgc cgggcgcgtc ctcgggcgcc cagccgatga acgactctcc
      601 gcggagctcg agctcgatct cgcaacgtcg ctgcagcacg agcgcaccga acatcttggc
      661 gatgttgccc gcgttgggat cgggattcgc agcgatgcgg ttggcgagca gccccgagac
      721 cacgttgtcg accaccacgt gcgcgatgag ctgccgaacg tggggatcgc tgccgcgttc
      781 gctcgcgttc accaagtcga tcagctcgcg accgggatcc gggtcgccgc cgcggtcctt
      841 cgcgaacgtc gggcccgtga gaccggcgcc cgacgtcatg agccgtgagt acttgaacat
      901 ggcggacgcg acgggccacc caccattgac cttgccgagc acggcatcgg cgcccaatcg
      961 gacgtcgtcg aagaactcct gacagaactc ctcgtcgccg ctcatctggc gcaacgggag
     1021 gagcgtcacg ccgggcgtgt cgaggcgcat gacgaacatg gtgaggccgg cgtgcttcgg
     1081 cacgtcccaa tcggtccggg cgagcaccat cgcgtgatcc gaccactgcg cgaagctgct
     1141 ccacaccttc tcgccgttga ggatccactc gtcgccgtcg cgttcggcgc gcatccgcgc
     1201 cgccgcgaga tccgagcccg caccgggttc ggagagcagc tggacccaga cgctctcgcc
     1261 gcgcagcatc tccgggagca gaccttcctt ctgctcctcg gacccgtgct cgaggatcgt
     1321 catgccgagg atggcgagcg tgatctggaa cgcgtccgaa gggagctcgt atccagctgc
     1381 ttcgtcgttg aacaccatct ggtgcgcaag cgagagaccc tgaccgccgt atcgctcgtc
     1441 ccacgcgatg ccggcgaaac cggcggcgtg ctgcatccgt tggaacgaac gggcccgcga
     1501 ccagtattcg ggatcctgct gatccgcgac cgtgaggcgg cgcggggcgt gatcggccag
     1561 ccagacgcga gcgcggtcgc ggaagtactc gggatcgacg tccttcgggt cggtgggcat
     1621 caggcggcga cccccacgac gtcacacaaa cgatcggcgt gccatgtcgg agaaccgaag
     1681 agcatgcggt tcgactgcac gcgccggagg tagaggtggg catcgtcttc ccacgtcatc
     1741 gcaatcccgc cgtgtacctg catgcacatc tccaccacgc gcggacactc cgtgccgacg
     1801 aacaccttcg cgacgtgcac ggcgcgcgcc gcgtcgctcg cgtcggccgc aaccgcgcgc
     1861 gcggcggccc acgtggcggc ctgggctccc tcgacgagca tgagtgcctc ggcgaggtgg
     1921 tgcttcaccg cctggaacga accgatcggc cggccgaacg cgacgcgctc cttggcgtat
     1981 tcgacggatc gctcgagcat ccgaccagtg gcgccgaccg actctgcgca ctgcaacacc
     2041 acggccagtc gcagctggac gtcgacggcg gatgcgtcgc tcggtgcacc gacgaccgcg
     2101 tcgaacggca cctgcacttc gtcgaagtga acgcgggcga gtcgtcggcc cagatccatc
     2161 gtctcgagcg ggacgatcat gacgcccggc gtgtcgacgc tcacgaggag ctgcaccact
     2221 cggccgcgct catcgcggca tgtcacgagc aggatgtcgg cgttctgtgc gtcctgcacc
     2281 ca
//
LOCUS       DASUGA010000699         4888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1320732, whole genome shotgun sequence.
ACCESSION   DASUGA010000699 DASUGA010000000
VERSION     DASUGA010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320732"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VFX21_13875"
     CDS             complement(<1..993)
                     /locus_tag="VFX21_13875"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5097109.1"
                     /translation="MEPACAPVRPALDGASVAGLVPALIGGADDAWLPAPARAAEQVV
                     LLVLDGLGWNALQAHAAQMPTLASMAGGPITTIVPSTTSAALTSIATGLAPAQHGILG
                     YRMVVGGQVLNVLRWTVANSKRAPDPFDVQRHTAFAGREVPVVTKSEFRTSGFSQAHL
                     RGGRFLGWHTTASLVEHVVQCVAHGERFVYAYYPGVDTVAHEFGLRDGFFTRELAFAD
                     ALVTDLLDALPARAALLVTADHGQMHLERDDWIELTPLLPMVAMMAGDGRFRYLHAKK
                     GEANQLLEDARDLVGANAWVRSRAEVLAEGWIGRGATGSVPGRLGDVVLASATNA"
     gene            1025..1432
                     /locus_tag="VFX21_13880"
     CDS             1025..1432
                     /locus_tag="VFX21_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX5097110.1"
                     /translation="MKVSTRGDYAARALLSLALHGSDRPTSVKEIAERTTLPQPYLEQ
                     ILLAVKGAGLVRSKRGVGGGYVLARPADEITLADIVAAVEGPQLPLAEHPDHCEGHCV
                     LQEVWIVVSEESRRALERVTLAELVERTRVGHS"
     gene            complement(1445..2371)
                     /locus_tag="VFX21_13885"
     CDS             complement(1445..2371)
                     /locus_tag="VFX21_13885"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX5097111.1"
                     /translation="MTASEPLPIPASLDGERVDRAVALLTGWSRADVQTLVERGDVLV
                     DGSEVGKSYRLRTGNVVELLAEPEADAPPGPEPEVAVEVRHADDDVIVVAKPAGVVVH
                     PGAGHAHGTLVNGLLARYPEIAAVGEPMRPGIVHRLDRDTSGLLVVARTAAAYEHLVG
                     QLATRTMERQYIALVWGRPSSPRGVVDAPIGRSETRRTRMAVRDAGRPARTAYEVQES
                     FDDPLTSLVYCRLETGRTHQIRVHLAAIGHPVVGDGTYGGKRDRIALDRPFLHAAVLG
                     FEHPATGAHVRFEEPLPPDLIAVLARLQDENA"
     gene            complement(2368..2910)
                     /gene="lspA"
                     /locus_tag="VFX21_13890"
     CDS             complement(2368..2910)
                     /gene="lspA"
                     /locus_tag="VFX21_13890"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX5097112.1"
                     /translation="MASGAADATAAPPRTRYLLIGVALLVIVALDQLTKTWVVRALAD
                     GPVVVVDEWLEFHLAKNSGSAFGRFQNFTPVLATLAIVVAVLLFRMARKETDRVMLIG
                     LVLILGGALGNLSDRIFRAPGFMRGHVIDFVAVGPWPLFNVADSCVTIGAIVLIVRTL
                     WPPKPAEPADPAEPVQPASP"
     gene            complement(2891..3658)
                     /locus_tag="VFX21_13895"
     CDS             complement(2891..3658)
                     /locus_tag="VFX21_13895"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX5097113.1"
                     /translation="MARATKAAAPAKKTATKKATAAKKAAPAKTKTPARKAAAPTKKA
                     APAKAAKAPAKKAAPPAKKAAPAKKAPVKVAKAAPAPAKKAPVKVAKATAPAKKAAPV
                     KAAAPVKKAPKPLAEPAPPPPPKPKKLPPPLAPSTLDKLRERLVEERDRHRRQAEELQ
                     AEAEVLATEREQGDTQFDEESGEGDTVSVERERDLALSASARQTVDDIERALAKIDDK
                     TFGLCATCGDRIPVARLEVIPWAEQCVKCKARGERRR"
     gene            complement(3958..>4888)
                     /locus_tag="VFX21_13900"
     CDS             complement(3958..>4888)
                     /locus_tag="VFX21_13900"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX5097114.1"
                     /translation="APVVSMAAVPGGSGYWLLAQDGGVFSFGSAVFHGSTGSMRLNAP
                     VVGMTPTASSGGYTLVAEDGGVFSFGDSVFQGSAAGQLKPGRRATQVLGMPNGDGYRV
                     LALARPADVALVGMGATGPGVTDLQMRLESLGFWTGGVNGTYGPLTQQAVWAFQKANN
                     LNRSGVVDPATRVAFRTARRPVPRTTSGYTIEVDKGRQLVIVARDGRAQYVLNTSTGT
                     ERPYTFEGVQYMADTPPGHWRVTSQVDGYKDGPLGTLFRPKYFHPDGIAFHGYTSVPP
                     TPASHGCVRLSNAAINWVWDNNIIPIGTDVWVY"
BASE COUNT          664 a         1717 c         1750 g          757 t
ORIGIN      
        1 ggcgttcgtc gccgacgcga gcacgacgtc gcccaagcgg cccggcaccg agcccgtcgc
       61 gcccctgccg atccagccct cggcgagcac ctccgcccgc gatcgcaccc acgcgttcgc
      121 accgacgagg tcgcgcgcgt cctcgagcag ctgattcgcc tcacccttct tcgcatgcag
      181 gtaccggaac cggccgtcgc ccgccatcat cgcgaccatc gggagcaacg gcgtcagctc
      241 gatccagtcg tctcgttcca agtgcatctg gccgtggtcg gcagtgacca gcagcgccgc
      301 gcgcgcgggt agagcatcga gcaggtcggt gacgagcgcg tcggcgaacg cgagctcgcg
      361 cgtgaagaac ccgtcgcgca gcccgaactc gtgcgcgacc gtgtcgacgc ccgggtagta
      421 cgcgtagacg aagcgttcgc cgtgcgcgac gcactggacg acgtgttcga ccagtgacgc
      481 agtggtgtgc cagccgagga atcggccgcc gcgcagatgc gcctggctga agccgctcgt
      541 gcggaactcg ctcttcgtca cgaccggcac ctcgcggccc gcgaacgcgg tgtgccgttg
      601 gacgtcgaag ggatcgggcg cgcgcttcga gttggcgacc gtccatcgca acacgttcag
      661 cacctgaccc ccgacgacca tccggtagcc gaggatcccg tgctgggccg gtgcgagacc
      721 ggttgcgatc gacgtcagcg cggcggaggt cgtcgacggc acgatcgtcg tgatcggtcc
      781 gcccgccatg gacgcgagcg tcggcatctg tgccgcgtgc gcttggagcg cgttccagcc
      841 gagcccgtcg agcacgagga ggacgacctg ttcggcagcg cgcgcgggcg cgggcagcca
      901 cgcgtcgtcg gccccgccga tcagggcggg gaccaggccc gcgaccgacg cgccgtcgag
      961 tgcgggtcga acgggcgcgc atgcgggttc cacctccctc gcaatgtacg cgaccattga
     1021 ggtcatgaag gtctccaccc ggggtgacta tgcggcgcgc gcactgctgt cgctcgcgtt
     1081 gcacggcagc gaccggccca cctcggtgaa ggagatcgcc gagcgcacga cgctccccca
     1141 gccgtacctc gagcagatcc tgctcgcggt caaaggggcc gggctcgtcc gctcgaagcg
     1201 aggtgtcggc ggcggctacg tgctcgcccg gcccgcggac gagatcacgc tcgccgacat
     1261 cgtcgccgcg gtcgaaggtc cgcagctccc gctcgcggag caccccgacc actgcgaggg
     1321 ccactgcgtg ctgcaagagg tgtggatcgt cgtgtccgag gaatcgcgcc gcgcgctcga
     1381 gcgcgtcaca ctcgccgagc tcgtcgaacg cacccgcgtc gggcacagct gacgatcgat
     1441 cgcgttacgc gttttcgtcc tgcagtcgcg ccaacacggc gatgaggtcg ggtggtaacg
     1501 gctcttcgaa gcgcacatgc gcgccggtgg ccgggtgctc gaacccgagc accgccgcgt
     1561 ggaggaacgg gcggtcgagc gcgatgcgat cgcgtttgcc gccgtaggtg ccgtcgccga
     1621 caaccggatg cccgatcgcg gcgaggtgca cccggatctg gtgggtgcgg ccggtctcga
     1681 gccggcagta cacgagtgag gtgagcggat cgtcgaacga ttcctgcacc tcgtacgcgg
     1741 tgcgggccgg ccggcccgcg tcgcgcacgg ccatgcgggt gcggcgtgtc tcggagcggc
     1801 cgatcggcgc gtcgacgacg ccgcgcggtg acgacggccg tccccacacg agcgcgatgt
     1861 actggcgctc catcgtgcgt gtcgcgagtt gtccgacgag atgctcgtac gcagcggcgg
     1921 tgcgggcgac gacgagcagc ccgctcgtgt cgcggtcgag gcggtgcacg atgccgggcc
     1981 gcatcggctc gcccaccgcc gcgatctccg ggtatcgcgc gagaagcccg ttgacgagcg
     2041 tgccgtgcgc atgcccggcg ccggggtgca cgacgacccc ggccggcttg gccacgacga
     2101 tgacgtcgtc gtcggcgtgg cgcacctcga cggcgacctc gggttcgggg ccgggtggcg
     2161 cgtccgcctc cggttcggcg agcagttcga ccacgttgcc ggtgcgcagc cggtagctct
     2221 tgccgacttc gctgccgtcg acgagcacgt cgccgcgctc cacgagcgtt tggacgtcgg
     2281 cgcggctcca gccggtcaag agcgcgacgg cgcggtcgac gcgctcgccg tcgagcgacg
     2341 cggggatcgg cagcggctcg ctcgcggtca cgggctcgcc ggctgcaccg gttcggccgg
     2401 gtcagccggc tccgccggtt tgggcggcca caacgtgcgg acgatgagca cgatcgcacc
     2461 gatcgtgacg cacgagtccg cgacgttgaa caacggccac ggaccgacgg cgacgaagtc
     2521 gatgacgtgg ccgcgcatga agcccggcgc gcggaagatg cggtcgctca ggtttccgag
     2581 cgcgccgccg aggatgagca cgagcccgat gagcatgacg cggtcggtct ccttgcgcgc
     2641 catgcgaaag agcaggaccg cgacgacgat tgccagcgtc gcgagcaccg gtgtgaagtt
     2701 ctggaagcgg ccgaacgcgc tccccgaatt cttggcgagg tggaactcga gccattcgtc
     2761 gacgacgaca actgggccgt ccgcgagcgc gcgcaccacc cacgtcttcg tgagctggtc
     2821 gagcgcgacg atgacgagga gcgcgacgcc gatcaacaga tagcgcgtgc gcggcggagc
     2881 ggccgtcgcg tcagcggcgc cgctcgccac gggccttgca cttcacacac tgctccgccc
     2941 acggaatcac ttcgaggcgg gcaacgggga tgcggtcccc gcacgtcgca caaagaccga
     3001 acgtcttgtc gtcgatcttc gccagcgcgc gctcgatgtc gtcgacggtc tggcgcgcgc
     3061 tcgccgacaa cgcgaggtcg cgttcgcgct cgacgctgac cgtgtcgcct tcgcccgact
     3121 cttcgtcgaa ctgcgtgtct ccctgctcgc gttcggtggc gagcacctcg gcctcggcct
     3181 gcagctcttc ggcctggcgg cggtgccggt ctcgttcctc gaccaggcgc tcgcgcagct
     3241 tgtcgagcgt gctgggcgcg agcggcggtg gcagcttctt cggcttcggg ggcggcggcg
     3301 gtgccggctc ggccaagggc ttcggcgcct tcttgaccgg cgccgcggcc ttcacggggg
     3361 cggccttctt cgccggcgcc gtggccttgg ccaccttgac cggcgccttc ttggccgggg
     3421 cgggtgcggc cttggccacc ttgaccggcg ccttcttggc cggggcggcc ttcttggccg
     3481 gcggggccgc cttcttcgcg ggggctttcg ccgccttggc cggcgccgcc ttcttggtgg
     3541 gggcagcggc cttcctcgcc ggggtcttgg tcttggcggg cgccgccttc ttggccgccg
     3601 tcgccttctt ggtggccgtc ttcttggcgg gtgccgccgc cttcgtcgcc ctggccatat
     3661 tgcccttctc gtccgattct cacggagtta aggctccgcg aggcgccgga gtgtagcggg
     3721 cgcgtcaaga cccgcggttc atgctggcct cgtcgctccg ggcgtgttcg cgcgcacgac
     3781 ttcggtgcct cgccacgccc tccgactcca cgagtggccg agacggtcgt gagggcgctt
     3841 cgcgccggag cagacaccga ggccaccgcc tcgtgcgact tctcgcgccg gtcctcgagg
     3901 ggcgctccgc gccgcgggag gaccgaacat cgagatcact ctcggccggt cacgcgccta
     3961 atacacccag acgtcggtgc cgatggggat gatgttgttg tcccagaccc agttgatggc
     4021 ggcgttgctg aggcgcacgc agccgtgcga cgccggggtc ggcgggacgc tcgtgtaccc
     4081 gtggaacgcg atgccgtcgg ggtggaagta cttcggccgg aagagcgtgc cgagcggacc
     4141 gtccttgtag ccgtcgacct ggctcgtcac gcgccagtgg ccgggtggcg tgtccgccat
     4201 gtactgcacg ccttcgaacg tgtacggccg ttcggtaccc gtcgacgtgt tgaggacata
     4261 ctgcgcgcgc ccgtcgcggg ccacgatcac gagttggcgg cccttgtcga cctcgatcgt
     4321 gtaaccgctc gtcgtgcgcg gcaccggccg gcgtgcggtg cggaacgcga cgcgcgtcgc
     4381 ggggtcgacg acgccactgc ggttcaggtt gttcgccttc tggaaagccc agactgcttg
     4441 ttgggtcagc gggccgtacg tgccgttgac gccaccggtc cagaagccca acgactcgag
     4501 gcgcatctgc aggtcggtca caccgggacc cgtcgcgccc atgccgacga gtgcgacgtc
     4561 cgcgggacgc gccagcgcga gcacgcggta gccgtcgccg ttgggcatgc cgagcacttg
     4621 cgtcgcgcgc cgaccgggct tgagttgccc ggccgcgctg ccttggaaca cggagtcgcc
     4681 gaagctgaac acaccaccgt cttcggcgac gagtgtgtaa ccgcccgacg acgcggtcgg
     4741 cgtcatgccg acgacgggcg cgttgagcct catgctgccg gtcgagccgt gaaacaccgc
     4801 gctgccgaac gagaacacac caccgtcttg cgcgaggagc cagtagccgg agccgcccgg
     4861 caccgcggcc atcgagacga ccggcgcg
//
LOCUS       DASUGA010000700         2529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1320536, whole genome shotgun sequence.
ACCESSION   DASUGA010000700 DASUGA010000000
VERSION     DASUGA010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320536"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VFX21_13905"
     CDS             <1..888
                     /locus_tag="VFX21_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097115.1"
                     /translation="HFGLDHAYEFFDGTSACNDPMTYRTDCGGEKFFRNKAAKCGEDK
                     VRDCFCGGLQNSHAKILGVFGAGQSTIAPPTVVVNSPMNGATAIEGQVIAFQAGSKRG
                     VEKSELYLNGWKWGEVKGAAFGGNGQLNPSNYSTKFPAGVPNGVIDIEIKAYDDLNIS
                     TTSQKVTVTKGMPCANAMSDCAPGQKCEAGKCFWDPPTGKLGDTCEYQQFCESNQCVM
                     TDEGGFCSQECIVGAMGACPDGFECAQNGSTGACLPVSSGGGCCSVSGSDAMWVHGGL
                     SMFVLGLVMRRRRRSRNRL"
     gene            898..2328
                     /locus_tag="VFX21_13910"
     CDS             898..2328
                     /locus_tag="VFX21_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097116.1"
                     /translation="MRFWLASLPLLLIARSAGAEPRRVTVDTPVSAFAHPGAIAPYLF
                     LNRCVGGCAITGATTNDAAAHWTTIPSPGSYVVHEFATATGETGTAADADWAAVVQCM
                     KEVYSPYAITVSDAPPPPGTAFNEAIIAGQPTDVGLGFDILGISPLANDCSPQDNVLS
                     FSFANHHGPDARALNICWTASQESAHAYGLDHEYEFSDGNSACSDPMTYRTDCGGQKF
                     FRNKRAKCGEMAARKCRCNSSQNSHTQLISIFGAGVPITAAPHASVIFPTNNSAVTTG
                     WVTHISAGSQRGVAKVELYFNDSRWMVQPGAKFGANGQLDPSTYTFIPPANVPDGIID
                     IQGKVYDDLGIGVATIPIRVTKGAPCTTDDQCAAHQHCDAGRCLFEAPVGELGDSCEY
                     PQYCKSWTCLGDGDKTCTQECRTDEPTSCPEGLECFSASENGVDGFCFPPGGGCCSVG
                     HERHAIWIHGGLALAVLGSLRRRRRR"
     gene            2341..>2529
                     /locus_tag="VFX21_13915"
     CDS             2341..>2529
                     /locus_tag="VFX21_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097117.1"
                     /translation="MVRGVCALLLLAALASPVAADTTSTTSVSQLMVLEPGDASYKLF
                     HGAIWLDIDKATTNYRWGG"
BASE COUNT          440 a          804 c          847 g          438 t
ORIGIN      
        1 cacttcggtc tcgatcacgc gtacgagttc ttcgacggca cgtcggcgtg caacgatccg
       61 atgacgtatc gcaccgactg cggcggcgag aagttcttcc gcaacaaggc cgcgaagtgc
      121 ggtgaagaca aggtgcgcga ctgcttctgc ggtggtctgc agaactcgca cgcgaagatc
      181 ctcggcgtgt tcggtgccgg tcagtcgacg atcgcgccgc cgaccgtcgt cgtgaactcg
      241 ccgatgaacg gcgcgaccgc gatcgaaggt caggtcatcg cgttccaggc aggcagcaag
      301 cgcggcgtcg agaagtcgga gctctacctc aacggttgga agtggggcga ggtcaagggc
      361 gcggcgttcg gtggcaacgg ccagctcaat ccgtcgaact actcgacgaa gttcccggcc
      421 ggtgtgccaa acggtgtgat cgatatcgag atcaaggcgt atgacgacct caacatctcg
      481 accacgtcgc agaaggtgac cgtgacgaag ggcatgccgt gcgcgaacgc catgagcgac
      541 tgcgcgccgg gtcagaagtg cgaagccggt aagtgtttct gggatccgcc caccggcaag
      601 ctcggcgaca cgtgtgagta ccagcagttc tgcgagtcca atcagtgcgt catgaccgac
      661 gagggtggct tctgctcgca ggagtgcatc gtcggcgcga tgggtgcgtg cccggacggc
      721 ttcgagtgcg cccaaaacgg ctcgacgggt gcgtgtctgc cggtatcgag cggcggcggt
      781 tgctgcagtg tttctggtag cgatgcgatg tgggtgcacg gcgggctgtc gatgttcgtg
      841 ctcggcctcg tgatgcgccg tcgtcgtcgg tcgcgcaatc gtctatgatt taggccaatg
      901 cggttctggc tcgcctcgct gccgctcctg ctgattgcac gaagcgcagg agccgagccc
      961 cggcgcgtca ctgtcgacac accggtcagt gcgttcgcgc acccgggcgc catcgcgccg
     1021 tatctgttct tgaaccgctg cgtcggcggc tgtgcgatca cgggcgcgac aacgaacgat
     1081 gccgccgcgc actggacgac gattccaagc cccggttcgt acgtcgtgca cgagttcgcg
     1141 actgccaccg gtgagaccgg cacggctgcc gacgccgact gggccgccgt cgttcagtgc
     1201 atgaaagagg tctactcgcc gtacgcgatc acggtgagtg acgcgccgcc gccgccgggg
     1261 accgcgttca acgaagcgat catcgccggt caaccgaccg acgtcgggct cgggttcgac
     1321 atcctcggca tctcgccgct cgcgaacgac tgcagcccgc aagacaacgt gctctcgttc
     1381 tcgttcgcga accaccacgg ccccgacgca cgtgcgctga acatctgctg gacggcgtcg
     1441 caggagagcg cgcacgcgta tggcctcgac cacgagtacg aattctccga cggcaactcg
     1501 gcgtgcagcg atccgatgac gtatcgcacc gactgcggtg ggcagaagtt cttccgcaac
     1561 aagcgcgcga agtgcggcga gatggcggcg cgcaagtgcc gttgcaactc gtcgcagaac
     1621 tcgcacacgc agctgatctc gatcttcggc gccggggtgc cgatcactgc agcgccgcac
     1681 gcgtcggtga tctttccgac gaacaactcg gccgtgacca ccggctgggt cacgcacatc
     1741 tcggccggct cgcagcgcgg cgttgcgaag gtcgagctct atttcaacga cagtcgctgg
     1801 atggtgcagc ccggcgcgaa attcggcgcc aacggccaac tagatccgtc gacgtacacg
     1861 ttcattccgc cggcgaatgt cccggacggc atcatcgaca tccagggcaa ggtctacgac
     1921 gacctcggca tcggggtcgc gacgatcccg atccgcgtca ccaaaggtgc gccgtgcacg
     1981 accgatgacc agtgcgccgc ccaccagcac tgtgacgcag gtcgctgtct gttcgaggcg
     2041 ccggtcggcg agctcggcga ttcgtgcgag tacccgcagt actgcaagag ctggacgtgc
     2101 ctgggcgatg gcgacaagac ctgcacccag gagtgccgga cggacgagcc gacgagctgc
     2161 cccgagggac tcgagtgctt cagcgcatcg gagaacggcg tcgacggctt ctgtttcccg
     2221 ccgggtgggg gctgctgcag cgtaggtcac gaacgccacg cgatctggat ccacggtggc
     2281 ctcgcactcg ccgtgctcgg atctctgcgg cggcgccgcc gccggtagta tgctgcggcc
     2341 atggttcgtg gtgtttgtgc gttgctgttg ctagcggctc tcgcgagccc cgtagctgcc
     2401 gatacgacgt cgacgacgtc ggtctcccag ttgatggtgc tcgagccggg cgacgcgagc
     2461 tacaagctgt ttcacggcgc gatctggctc gacatcgaca aggcgacgac caactaccgg
     2521 tggggagga
//
LOCUS       DASUGA010000701         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_765888, whole genome shotgun sequence.
ACCESSION   DASUGA010000701 DASUGA010000000
VERSION     DASUGA010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765888"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..630)
                     /locus_tag="VFX21_13920"
     CDS             complement(139..630)
                     /locus_tag="VFX21_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097118.1"
                     /translation="MAEEDMVKAVQAALGDHGIDDTIREVGHFNPRGMTGGLFAGGMA
                     ASEAGSVMGGVGEAVGLAAGSLGGMEAASMASGLPRNMLVGASDTMVYGFKMASGGRK
                     KEPHELLFQVARAGLEVKVHQRVNVRVLELIHPESGSTIELEGNRLPITHSKDVIEFL
                     EGK"
     gene            724..1869
                     /locus_tag="VFX21_13925"
     CDS             724..1869
                     /locus_tag="VFX21_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006379940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5097119.1"
                     /translation="MDFSLTEGERDLVHLCREFAQKEIAVRAPVAWEEGRCPTDLLRE
                     MGALGLLGMLIPEAWGGIGMSTVGFVAALEQLGLADQSVAAAFQAHVTIGSLPLYLFG
                     TDAQRERWLRPLAEGRALGAFGLTEPDAGSDARGIRTRATRRDGGWVINGRKTFISNA
                     GTDMSFGVTLLARTDSGDDKPTYATFVVENGTDGFTTGPKMRGIGWRGLDTRELYFDD
                     VWVDDDHLLGDPAMGLTQFLRTLEVGRISIAALSLSLTQAVLDLATAYAKEREQFGRP
                     IAQFQAVQFKLADIATELEAARWLTYRAAALRDAGEPFVKEAAMAKLKASRLAVSAAS
                     EAVQIHGGLGYMLDAPVARFYCDAKVLEIGEGTNEIQHVVIARELLR"
     gene            complement(1866..>2131)
                     /locus_tag="VFX21_13930"
     CDS             complement(1866..>2131)
                     /locus_tag="VFX21_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016433408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5097120.1"
                     /translation="KDDFFPFLERLQPVVHEMRGWTADEIRSITAPTFLVIGDHDFVR
                     LDHAAEMLDLFPDCRLAVLPATKHTEIMERTGALRTLVAPFLS"
BASE COUNT          343 a          730 c          706 g          352 t
ORIGIN      
        1 gatcgccggt gtcctgatga cgtcgttgcc gaacgcgccc acacagtccg cgcgcagctg
       61 gtcgggcatg aacagcagaa gattcggccg atcgttcacg cggcgatcat gccagaaggc
      121 ggaggatcct cgggcgggtc acttcccttc gaggaattcg atcacgtcct tcgagtgggt
      181 gatcggcagc cgattgcctt ccaactcgat cgtcgaaccc gactcggggt ggatcagctc
      241 gagcacgcgc acgttgacgc gctgatgcac cttcacctcg agtcccgcgc gcgccacttg
      301 gaacagcaac tcgtgcggtt ccttcttgcg accgccggac gccatcttga agccgtagac
      361 catcgtgtcg gacgcgccga cgagcatgtt gcgcggcagt cccgacgcca tcgacgccgc
      421 ttccatcccg cccagcgatc ccgcggcgag gcccaccgcc tcgccgaccc cgcccatgac
      481 cgacccggct tcgctcgcgg ccatccctcc cgcgaacaac ccgccggtca tgccccgggg
      541 attgaagtgg ccgacttcgc gaatcgtgtc gtcgatgccg tgatcgccca gcgcggcctg
      601 gaccgctttg accatgtcct cttctgccat gccgtcgacc caagcacgcc ggacggcgcc
      661 gcgcgtcacc cgcggcaggt ggtgtcggac gcgctgtacc ggttggtcca atagtctccg
      721 atcgtggact tctcgctgac cgagggcgaa cgcgacctcg tgcacctgtg ccgcgagttc
      781 gcacagaagg agatcgcggt ccgggctccg gtcgcatggg aggaagggcg ctgccctacc
      841 gatctgctgc gcgagatggg cgcgctcggg ctgctcggca tgttgatccc cgaagcgtgg
      901 ggcggcatcg gtatgtcgac ggtcggcttc gtcgccgcgc tcgaacaact cggcctcgcc
      961 gaccaatcgg tcgcggctgc gttccaggcc cacgtcacga tcggctcgct gccgctctac
     1021 ctcttcggca ccgacgcgca acgcgagcga tggctgcgcc cgttggccga aggacgcgca
     1081 ctcggcgcct tcgggttgac cgaacccgac gccggatccg acgcgcgtgg cattcgtacg
     1141 cgcgcgacgc ggcgcgacgg cggctgggtg atcaacggcc gcaagacgtt catctccaac
     1201 gcgggcaccg acatgtcgtt cggtgtgacg ctgctggcgc gtaccgattc gggcgacgac
     1261 aagcccacct acgcgacgtt cgtcgtcgag aacggcaccg acggcttcac gacgggcccg
     1321 aagatgcgcg gcatcggctg gcgcggcctc gatacgcgcg agttgtactt cgacgacgtc
     1381 tgggtcgacg acgatcacct actgggtgac cccgcgatgg ggctcaccca gttcctgcgg
     1441 acgctcgaag tcggccgcat ctcgatcgcg gcgctgtcac tcagcctgac ccaggcggtg
     1501 ctcgacctcg cgaccgcgta cgccaaggag cgcgagcagt tcggccggcc gatcgcgcag
     1561 ttccaggccg tccagttcaa gctggccgac atcgcgaccg agctcgaggc ggcccgctgg
     1621 ctgacgtatc gcgcggcggc gttgcgcgac gcgggtgaac cgttcgtcaa agaggcggcg
     1681 atggcgaagc tgaaagcgag tcgtctcgcg gtgtccgccg cttccgaagc cgtacagatc
     1741 cacggcggcc tcggctacat gctcgacgca cccgtggcgc gcttctactg cgacgcgaag
     1801 gtgctggaga tcggtgaagg cacgaacgag atccagcacg tcgtgatcgc gcgcgagctc
     1861 ctgcgttagg aaaggaacgg cgcgacgagc gtacggagcg cgccagtgcg ttccatgatc
     1921 tccgtgtgct tcgttgcggg cagcactgcg agccggcagt cggggaacag atcgagcatc
     1981 tcggccgcgt ggtcgaggcg cacgaagtcg tggtcgccga tcacgaggaa cgtcggcgcc
     2041 gtgatgctgc ggatctcgtc cgcggtccaa ccgcgcatct cgtgcactac cggctgcaac
     2101 cgttcgagga acgggaagaa gtcgtccttg t
//
LOCUS       DASUGA010000702         4022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_210961, whole genome shotgun sequence.
ACCESSION   DASUGA010000702 DASUGA010000000
VERSION     DASUGA010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210961"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VFX21_13935"
     CDS             <1..275
                     /locus_tag="VFX21_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020519902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEX5097121.1"
                     /translation="IVDDGIATGSTARVACAVARAAGAREVVLAVPVAATATVAALRA
                     DPDIDEIVAVLTPAHFLAVGQWYRDFAQTSDKEVSRLLRDARPGRS"
     gene            313..1194
                     /locus_tag="VFX21_13940"
     CDS             313..1194
                     /locus_tag="VFX21_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX5097122.1"
                     /translation="MQVHLVDGTYELFRQYLAPRPGHLDAGGVEVGGTRAVVLSVLSM
                     LEQGATHLGVATDHVIESFRNDLWDTYKTGEGMDPLLLAQFPLLEEALVALGVTVWPM
                     VDVEADDALASAARVAAADERVEQVIICTPDKDLGQCVGGKVVQLDRRREILIDAEGV
                     REKFGVPPASIPDWLALVGDSADGFPGLPGWGAKSAATVLDRYGHIEAIPDDAKQWDV
                     TVRGAEKLANTLATGRAVADRFKVLATLVLDAPVGTVDDWEWRGPTPELKAWAERFDS
                     PALLTRAEKLTRQREKQ"
     gene            1191..1997
                     /locus_tag="VFX21_13945"
     CDS             1191..1997
                     /locus_tag="VFX21_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5097123.1"
                     /translation="MNDAVRLERVQPAIAQVTLNRPAKLNAMNYALVQGLHDVLDELA
                     ADPSCRVVVLTGAGRGFCAGLDLSEGASPPYAAGMGRAQAGMTVQKLIAGLVPKMRAL
                     PQPVIAAVNGPASGGGLALALASDVRIAATSAKFNVAFVRVGLSGCDIGVSWLLPRLI
                     GVSRAFELLLTGRLIDAAEADRIGLVTRVVADGDVVASAFETAELIVGNSPFGVRMTK
                     EVMWSQLEIGSLQAGIDLENRTQILSSTTGDLTEAMSAFLEKRTPQFRDA"
     gene            2037..2798
                     /locus_tag="VFX21_13950"
     CDS             2037..2798
                     /locus_tag="VFX21_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018179216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5097124.1"
                     /translation="MGLAGRVALVTGGGRGIGRAISLALAEDGADVAVNYRRDEDAAR
                     ETVALIEKLGRKAVAYSASVDDYDACAAMVDAAVAALGPISILVNNAGIASRGLNVKK
                     TELAEIERVIDTHAIGSWTMSNLVIPSMRECARGDIVMISSNAVLSMGPNSAPYNMAK
                     AAQEALAWTLAKEERKNNIHVNVVAPGLVDTEMGRRLVKGAMGVEDIHTLDARAPFGH
                     VCTPEEVADVVRFVVSDGAAYLTGQRIGVDGGSFQ"
     gene            complement(2805..>4022)
                     /locus_tag="VFX21_13955"
     CDS             complement(2805..>4022)
                     /locus_tag="VFX21_13955"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5097125.1"
                     /translation="TEPGGTFAVSTTATTEPAAAAEELSGTEFVFDVQTHLLDFDLSD
                     DRATAGTRFAQGYPYASCGEDDWRACFGIDHWLEELFVRSDTTMAVISAVPILATPNP
                     LSIEVMQAAKEAVPRVCGDDGRVVLHGQVNPNVVEPAVFTEGMRRLATDHEIGAWKVY
                     THVPDAQGWWLDDHDADAVQCGNAFLDAVREIGPKIVCVHKGFGGGSEYSSPMEIGAV
                     AKANPDITFVVYHSGYDGRNEGPYDPGNPNNGIDRLLKSVDDAGVEAGANVYAELGST
                     WWSAMSDTTQAAHVLGKLLARFGEDRVLWGTDSIWYGSPQDQIQAFRTFQISAELQDT
                     YGYPELTDTLKRKVFGQNAAALYGVDPVPEKCAVNDAALEEYRAALAPKKSYGPGSYA
                     EAAKVIAAHGMRS"
BASE COUNT          600 a         1342 c         1441 g          639 t
ORIGIN      
        1 tgatcgtcga cgacggcatc gcgaccggct cgaccgcacg cgtcgcgtgt gctgtcgcgc
       61 gcgccgccgg ggcgcgagag gtcgtgctcg cggtcccggt cgcggcgacc gcgacggtgg
      121 ccgcgctgcg cgccgatccc gacatcgacg agatcgtcgc ggtgctcacg cctgcgcact
      181 tcctcgcggt cgggcagtgg tatcgcgact tcgcgcagac atcagacaaa gaggtcagcc
      241 gcctgttgcg cgacgcgcgc cccgggcgat cgtgagttgt gccgtcacgg ccgcgcctgt
      301 gaagctccgg ccgtgcaggt ccacctcgtc gacggcacgt acgagctctt ccggcagtac
      361 ctcgcgccgc ggcccggcca cctcgacgcg ggcggcgtcg aggtcggcgg gacccgcgcg
      421 gtcgtcttgt cggtcctgtc gatgctcgag cagggcgcga cccatctagg cgtcgcgacc
      481 gaccacgtga tcgagtcctt tcgcaacgac ctctgggaca cctacaagac cggcgagggc
      541 atggacccgc tgctgctcgc gcagttcccg ctgctcgagg aggcgctcgt ggcgttgggc
      601 gtgacggtgt ggccgatggt cgacgtcgaa gcggacgacg cgttggcatc cgccgcgcgc
      661 gtcgcggccg cggacgagcg cgtcgagcag gtcatcatct gcacacccga caaggacctc
      721 ggccagtgcg tcggcggcaa ggtcgtgcag ctcgaccgcc ggcgcgagat cctcatcgac
      781 gccgagggcg tgcgcgagaa gttcggcgtg ccgcccgcgt cgatccccga ttggctcgca
      841 ctcgtcggcg actccgccga cggcttcccg ggcctgcccg gctggggcgc gaagtcggcc
      901 gcgacagtgc tcgatcgcta cgggcacatc gaagcgattc ccgacgacgc gaaacagtgg
      961 gacgtgacgg tgcgcggcgc cgagaagctg gccaacacgc tcgcgacggg acgggccgtc
     1021 gccgaccggt tcaaggtgct ggcgacgttg gtgctcgacg cgccggtcgg gacggtcgac
     1081 gactgggaat ggcggggccc gacaccggag ctcaaggcgt gggccgagcg cttcgactcg
     1141 cccgcgttgc tcacgcgcgc cgagaagttg acgcgacagc gggagaagca gtgaacgacg
     1201 ccgtccgtct cgaacgggtg cagccggcca tcgcgcaggt cacactcaac cgccccgcga
     1261 agttgaacgc gatgaactat gcgcttgtgc aggggttgca cgacgtgttg gacgagctgg
     1321 cagccgatcc gtcgtgccgc gtggtggtgc tcacgggtgc ggggcggggg ttctgtgcgg
     1381 gcctcgacct gagcgagggt gcgagccctc cgtacgcagc cggcatgggt cgcgcccagg
     1441 cgggcatgac ggtgcagaag ttgatcgccg gcctggtgcc gaagatgcgc gcgctcccgc
     1501 agccggtgat cgcggcggtc aacggtcccg cgtccggtgg cggcttggcg ctcgcactcg
     1561 cgagcgatgt gcgcatcgcg gccacgagcg cgaagttcaa cgtcgcgttc gtccgcgtcg
     1621 gcttgtcggg gtgtgacatc ggcgtgtcgt ggttgctgcc gcgtctcatc ggtgtgtcgc
     1681 gcgcgttcga gttgttgttg accggccgcc tcatcgacgc cgcggaagcc gatcgcatcg
     1741 ggctggtgac gcgcgtcgtg gccgacggcg acgtcgtcgc gtccgcgttc gagaccgcgg
     1801 agttgatcgt gggcaacagc ccgttcgggg tgcgcatgac gaaagaggtc atgtggagcc
     1861 agctcgagat cggcagcctg caagcgggca tcgatctcga gaaccgcaca cagatcctgt
     1921 cgagcacgac cggtgacctg accgaggcga tgtcggcatt cttggagaaa cgcacaccgc
     1981 agttccgcga cgcatagttc gcgcgacgta gtccgcgagg catagaggga gcaacgatgg
     2041 gtttggcagg acgggtcgcg ctggtcacgg gcggggggcg cggcatcggc cgcgcgatct
     2101 cgctggctct cgccgaggac ggcgccgatg tcgcggtcaa ctaccgtcgc gacgaggacg
     2161 ccgcccggga gaccgtggcg ctgatcgaga agcttggccg caaggccgtc gcgtactcgg
     2221 cgtcggtcga cgactacgac gcgtgtgcgg cgatggtgga tgccgcggtc gcggccctcg
     2281 gcccgatctc catcctcgtc aacaacgccg gtatcgcgtc gcggggcctc aacgtgaaga
     2341 agaccgagct cgcggagatc gagcgcgtga tcgacacgca cgcgatcggg tcgtggacca
     2401 tgtcgaacct cgtcatcccg agcatgcgcg agtgtgcgcg gggcgacatc gtgatgatct
     2461 cgtccaacgc ggtgctctcg atgggcccga actccgcgcc gtacaacatg gccaaggccg
     2521 cgcaggaggc gctggcgtgg acgttggcga aggaggagcg caagaacaac atccacgtca
     2581 acgtcgtcgc gcccggcctc gtcgacacgg agatgggccg acgcctggtc aagggtgcga
     2641 tgggcgtcga agacatccac accctcgacg cccgagcgcc gttcggtcac gtgtgcacgc
     2701 cggaggaagt cgccgacgtg gtgcggttcg tcgtctccga cggcgccgcc tatctcaccg
     2761 gccaacgcat cggcgtcgac ggcggctcct tccagtaacg acggtcagga gcgcataccg
     2821 tgcgcggcga tgaccttcgc cgcttcggcg tagctgcccg gtccgtacga cttcttgggc
     2881 gcgagcgcgg cgcggtactc ctcgagcgcg gcgtcgttca ccgcgcactt ctcggggacc
     2941 gggtcgacgc cgtagagcgc ggccgcgttc tggccgaaga ccttgcgctt gagcgtgtcg
     3001 gtgagctcgg gatagccgta ggtgtcctgc agctcggcgc tgatctggaa cgtgcggaag
     3061 gcctggatct ggtcttgcgg tgagccgtac cagatcgagt cggtgcccca cagcacgcgg
     3121 tcctcgccga aacgcgcgag caacttgccg agcacgtgcg cggcctgcgt cgtgtcgctc
     3181 atcgcgctcc accacgtcga gcccagctcc gcgtacacgt tcgcgccggc ctcgacgccc
     3241 gcgtcgtcga ccgacttcag caagcggtcg atcccgttgt tgggattgcc cgggtcgtac
     3301 ggtccttcgt tccggccgtc gtagccggag tggtacacga cgaaggtgat gtcgggattt
     3361 gccttggcga ccgcgccgat ctccatcggc gacgagtact cgctgccgcc accgaagccc
     3421 ttgtgcacgc acacgatctt cgggccgatc tcgcgcacgg catcgaggaa cgcgttgccg
     3481 cactgcaccg cgtccgcgtc gtggtcgtcg agccaccacc cctgcgcgtc gggcacatgc
     3541 gtgtacacct tccaggcacc gatctcgtgg tcggttgcga gccggcgcat gccctcggtg
     3601 aacaccgcgg gttcgacgac gttgggattg acctgtccgt gcaagacgac gcggccgtcg
     3661 tcgccgcaca cgcgcgggac ggcctctttc gcggcctgca tgacctcgat cgacaacgga
     3721 ttcggcgtgg cgaggatcgg caccgccgag atgaccgcca tcgtcgtgtc ggagcggacg
     3781 aacaactcct cgagccaatg gtcgataccg aagcacgcgc gccagtcgtc ctcgccgcac
     3841 gacgcgtacg ggtagccctg cgcgaagcga gtgccggccg tcgcgcggtc gtccgagagg
     3901 tcgaagtcga gcaagtgggt ctgcacgtcg aacacgaact cggtgccgct gagctcttcg
     3961 gccgccgcgg ccggctccgt ggtcgcggtg gtggagaccg cgaacgtgcc gcccggctcc
     4021 gt
//
LOCUS       DASUGA010000703         2388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1841735, whole genome shotgun sequence.
ACCESSION   DASUGA010000703 DASUGA010000000
VERSION     DASUGA010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1841735"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="VFX21_13960"
     CDS             complement(<1..1496)
                     /locus_tag="VFX21_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097126.1"
                     /translation="MTHVRGRRAVGAVLAMLVVVAGCSSGGDSGGSGAPTASSNPGAS
                     KFSNEEITYGLAPENDDVTYQPDVVIVGGGADSIRSVSDDGIEWTIKGDAENIDKVEV
                     GKVMFVTGRAVGRVRQVRDAGDDVAVALGPVDLTEVYRDAEFSFDRPFDVTDLAYVET
                     PGYLEPVTPLDANTSAAEPSAAEVTTDVRAEPAVATETVTLPPLQFIANGRPAARGAE
                     IKGSLGGAFSVALDKWSVDALWSSNANGSIDLTFRVSRTEGPLKFGGVLVLHLADPTV
                     QASVKIKDGKVTSTKASFHGIKGVDVDLKSGSSGGSSDNVKLRFEVPLDFSIPVGAPY
                     PFLLSFKQKFALETAFSAKNSTLEGHGKYEFDGDVGSGGAASVAVKTVESMLDSIKGV
                     SVGVSGIVFSYGIRVGLGLGIPQFFAGPFAGATIAIGQSRGSDLGQVGAANLAGAVAT
                     GQNTVCRGVTLDLYASTGIAYNFSEAAQSVLDKVWPTSGKSVRQTGDF"
     gene            complement(1493..2035)
                     /locus_tag="VFX21_13965"
     CDS             complement(1493..2035)
                     /locus_tag="VFX21_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097127.1"
                     /translation="MTRVRMAATALVVLLALGATACGGDDDDTAAAETVAPTDATAAP
                     TTEAASTTPTTAAPADPCALVPKDKAEALAGTALLDGVLVSEETSSCTYTGPTDGPTA
                     QVEVFVGPGAKKNYDIDVSLEHAFTPVADLGDEGYIEDFTVFTRKGEEWVMIRLTLLE
                     DPPVYNEKLVALAREVAAEL"
     gene            complement(2032..2229)
                     /locus_tag="VFX21_13970"
     CDS             complement(2032..2229)
                     /locus_tag="VFX21_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097128.1"
                     /translation="MDIGGDEPGLAPAYQLALDLEAEGFEGPALAERLGVPEAALSSL
                     LRVAHAKAGTEARRTDERDDG"
BASE COUNT          367 a          837 c          785 g          399 t
ORIGIN      
        1 aagaagtcgc cggtttggcg aacggacttc cccgaggtcg gccacacctt gtcgagcacg
       61 gactgcgcgg cttcgctgaa gttgtacgcg atgcctgtgc tcgcgtacag gtcgagtgtc
      121 acaccacgac agaccgtgtt ctgaccggtc gccaccgccc cggcaaggtt cgcggcgccg
      181 acctggccga gatcggaacc ccggctctgc ccgatcgcga tcgtcgcacc ggcgaacggg
      241 cccgcgaaga actgcgggat acccaggccg aggcccacgc ggatcccgta cgagaacacg
      301 atgcccgaca caccgaccga cacacctttg atcgagtcga gcatgctctc gacggtcttg
      361 accgcgaccg acgctgcgcc gccgctgccg acatcgccgt cgaactcgta cttgccgtga
      421 ccttcgagcg tcgagttctt ggcgctgaac gcagtctcca acgcgaactt ctgcttgaac
      481 gagaggagga acggataggg cgcgcccaca gggatggaga aatcgagcgg cacttcgaag
      541 cggagcttca cgttgtcgct cgaaccgcca ctgctgcccg acttgaggtc gacgtcgaca
      601 cctttgatgc cgtggaacga cgccttcgta ctggtgacct tgccgtcttt gatcttcacc
      661 gacgcctgca ccgtcggatc ggcgagatgc aagaccagga cgccaccgaa cttgagcggg
      721 ccttcggtac gtgagacgcg gaaggtcagg tcgatcgagc cattcgcatt cgacgaccac
      781 aacgcgtcga ccgaccactt gtcgagcgca accgagaacg cgccaccgag actgcccttg
      841 atctctgcgc cgcgagcggc gggccggccg ttcgcgatga actgaagcgg cggcaacgtg
      901 acagtttcgg tcgcgactgc cggttccgcc cgcacatcgg tcgtgacctc ggccgccgac
      961 ggctcggccg cggatgtgtt cgcgtcgagc ggtgtcaccg gctcgaggta tccgggcgtc
     1021 tcgacgtacg cgaggtctgt gacatcgaag gggcggtcga agctgaactc cgcgtcgcgg
     1081 tacacctcgg tgaggtcgac gggcccgagc gcgacggcga cgtcgtcgcc ggcgtcgcgc
     1141 acctgccgga cgcgtcccac tgcacggccg gtcacgaaca tgaccttgcc gacctcgacc
     1201 ttgtcgatgt tctcggcgtc gcccttgatg gtccattcga tgccgtcgtc gctgaccgac
     1261 cggatcgagt ccgcgccgcc cccgacgatc acgacgtcgg gctggtaggt gacgtcgtca
     1321 ttctcgggcg cgagcccgta cgtgatctcc tcgttggaga atttgctcgc gccggggttc
     1381 gagctcgccg tcggcgcgcc gcttccgccc gaatcgccac ccgacgagca gccggccacg
     1441 acaacgagca tcgcgagcac cgcgccgacg gcgcgccggc cgcggacgtg ggtcacagct
     1501 cggctgcgac ttcgcgggcg agcgcgacga gcttctcgtt gtagacgggc ggatcctcga
     1561 gcagggtcag ccggatcatc acccactctt cacccttgcg tgtgaacacg gtgaagtcct
     1621 cgatgtagcc ctcgtcaccc aggtcggcga ccggtgtgaa cgcgtgctcg aggctgacgt
     1681 cgatgtcgta gttcttcttc gcgccgggac cgacgaagac ctcgacctgc gcggtcgggc
     1741 cgtcggtcgg gccggtgtac gtgcacgacg acgtctcttc cgacacgagc acgccgtcga
     1801 gcagtgccgt gcccgcgagg gcttcggcct tgtccttcgg caccagcgcg caggggtcgg
     1861 ccggtgctgc cgttgtcggt gtcgtgctcg ccgcctcggt cgtcggcgcc gcggtcgcgt
     1921 cggtgggcgc gaccgtctcg gccgccgcgg tgtcgtcgtc gtcaccgcca cacgcggtcg
     1981 cgccgagtgc gagcagtacc accagcgccg ttgccgccat acgaacgcgt gtcatccgtc
     2041 gtccctctcg tccgtccggc gcgcctcggt ccccgccttc gcgtgcgcaa cgcgcagcaa
     2101 cgacgagagc gcggcctccg gaacgcccag gcgttcggcg agtgccggcc cctcgaagcc
     2161 ctcggcctcg agatccagtg cgagctggta cgcgggcgcg agcccgggct cgtctccacc
     2221 gatatccacc cgccggacgg tacggatccg gcgcccggat gtctcgcgta cccgctacgc
     2281 gtttttctgc gcgtccagga cccgaccgag ctcgtgacgg gtggtcacac cgagcttggt
     2341 ggtcgcgcgc agcaggtgac cctcgaccgt acggacgttg acgccgag
//
LOCUS       DASUGA010000704         2174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_71340, whole genome shotgun sequence.
ACCESSION   DASUGA010000704 DASUGA010000000
VERSION     DASUGA010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71340"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..>2174)
                     /locus_tag="VFX21_13975"
     CDS             complement(129..>2174)
                     /locus_tag="VFX21_13975"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5097129.1"
                     /translation="VLVKGGPQQVTVAGIARFGKADSPGGASFVLFTTRAAQKLVAEP
                     GKFDGISVVANPGISQTEIARRIEPLLPGGTEVVTGVTVTKENQDAIRKGFSFFNTFM
                     LVFAIIALLVGAFIIFNTFFITVAQRTRENALLRALGARKRQVLLSVLIEALIVGVFA
                     SVIGVALGVFVAAALKGLLAAFGFDIPAGGVVLTTGTVIVSLIAGIGITLVAAISPAR
                     KAGKVPPVVAMRDVAVGSTGYGSKQRILAGAAVVILGIAALLYGLFGSADSPLPIVGA
                     GALLVFLGVAVLGRTVALPLSRFIGWPLPRLRGIRGQLARENAMRNPKRTAATASALM
                     IGVGLVAFITIFASSAKASFHHIIDTSFHGDFVVTSPASFGTGGIDPSLAAKLNGLPE
                     IAEAGSIRAGSAEIDGSSKFLVAASRETFDLVDVKPKQGSPDDLAADTIAVNDRTADD
                     KNLSIGDQVHVVFKDTGAKQLRVAMIYDEEQPAGQWLLGTKAYEANFRDQFDFQIFVR
                     KADNVDAAKALAAVKHAAAGYPGTKVLDQTAYIADQTKFIDQLLGLVYAMLLLAILIA
                     LLGIGNTLALSIIERTRELGLMRAVGMTRSQLRSTIRWESVIIALQGTLLGLVIGVFF
                     GWTLVRAMSDQGLTRFEVPAVSLLVIVVLAALAGVLAALWPARRAAKLNVLESIATE"
BASE COUNT          413 a          688 c          702 g          371 t
ORIGIN      
        1 cggttcaggc cgtcgacgtc ccgcttcggc ttcgtgagat cgaaggtcat gtactcccag
       61 gtctgccaat tctcgtcgtt ctcaactcga tcaatgggaa ccgccatgat gaactccttt
      121 tgcttgcgtc attcggtggc gatggactcg agaacgttga gtttggcggc gcgccgcgcc
      181 ggccacagtg ccgcaagcac gccggcgagc gcggccaaca ccacaatcac caacagggag
      241 acggcaggca cttcgaaccg ggtcaggcct tggtcggaca ttgccctgac gagtgtccac
      301 ccgaagaaca cgccgatgac aagaccgagc agtgtgccct gcagggcgat gatgacggat
      361 tcccatcgga tcgtcgagcg aagctgactg cgcgtcatgc ccaccgcacg catcaggccc
      421 agctcgcggg tgcgttcgat gatcgacagc gccagcgtgt tgccgatgcc gagcaacgcg
      481 atgaggatcg cgagcaagag catcgcgtag accaagccga gaagctggtc gatgaatttc
      541 gtttggtcag cgatgtaggc ggtctggtcg agcacctttg tccccgggta tccggcggcc
      601 gcgtgcttga ccgcagcgag cgccttcgcg gcgtccacgt tgtccgcctt ccggacgaag
      661 atctggaagt cgaactggtc tcggaagttc gcctcgtaag ccttggtgcc gagcaaccat
      721 tggccggcgg gctgctcctc gtcgtagatc atcgcgacgc ggagttgctt ggcgccggtg
      781 tccttgaaga cgacatgcac ctggtcgccg atcgacaggt tcttgtcgtc ggcggtcctg
      841 tcgttgacgg cgatcgtgtc cgcggcaagg tcatccggtg aaccctgttt cggcttcacg
      901 tcgaccagat cgaacgtttc cctactcgcg gcgacgagga acttcgacga cccgtcgatc
      961 tcggccgatc cagcgcggat cgacccggct tcggcgattt cgggaagccc gttgagcttg
     1021 gccgccaagc tcgggtcgat gccgccggtt ccaaacgaag caggtgaggt cacgacgaag
     1081 tcgccgtgaa acgacgtgtc gatgatgtga tggaacgagg ccttggccga cgacgcgaag
     1141 atcgtgatga acgcgacgag tccgaccccg atcatcagcg ccgacgcggt cgcggcggtc
     1201 cgtttcgggt ttcgcattgc gttctcgcgt gcgagttgtc cgcggatccc acgcaggcgg
     1261 ggcaggggcc agccgatgaa ccggctcagc ggcaacgcga cggtgcggcc cagcaccgcg
     1321 acgccgagga acaccaacag ggcgccggcg ccgactatcg ggagcggact atccgccgac
     1381 ccgaacaggc cgtacaagag tgcggcaatg ccgagtatca cgacggccgc gcccgcgaga
     1441 atgcgctgct tcgagccgta gccggtgctc ccgaccgcga cgtcgcgcat ggcgacgacg
     1501 ggcggcacct tccccgcctt acgcgccggt gagatcgctg cgacgagcgt gatgccgatg
     1561 ccggcaatga gcgagacaat cacggttccc gtcgtcagca caacgccacc ggcaggaatg
     1621 tcgaagccga atgcggcgag taaccccttc agcgcggccg cgacgaagac gccgagcgcg
     1681 acgccgatca ccgacgcgaa gacgccgaca atgagcgctt cgatcaggac cgaaagcagt
     1741 acctgccgtt ttctcgcacc gagcgcccgc agcaacgcgt tctcgcgggt gcgttgcgcg
     1801 accgtgatga aaaacgtgtt gaagatgatg aacgcaccga ccagcaaggc gatgatcgcg
     1861 aacacgagca tgaacgtgtt gaagaacgag aagccttttc gaatcgcgtc ctggttttct
     1921 tttgtgaccg tcactccggt cacaacttcc gttccgccgg gtagcagcgg ctcgatgcgc
     1981 cgcgcgatct ccgtctgtga gatgccggga ttcgcgacga ccgagatgcc atcaaacttg
     2041 cccggctcgg cgacgagctt ctgcgcggcg cgagtagtga acagcacgaa ggaggcaccg
     2101 ccgggagagt cggccttgcc gaacctcgcg atgcccgcca cggtgacctg ctgcgggcct
     2161 cccttgacga ggac
//
LOCUS       DASUGA010000705         3475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1913030, whole genome shotgun sequence.
ACCESSION   DASUGA010000705 DASUGA010000000
VERSION     DASUGA010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1913030"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VFX21_13980"
     CDS             <1..582
                     /locus_tag="VFX21_13980"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX5097130.1"
                     /translation="DYAIDAAFSGFSDQPTYEDARPIGEAATRAFLAEETDLVEIVYT
                     RFASAGIQVVERQTLVPMVRPEVTTDHEGGVPASYEFEPSPDSILDLLLPRYVDSRVF
                     AALLNAAASEHASRQRAMKSATDNAGDLIISLSRVMNRARQDAITTEIMEIVSGAEAL
                     RGSKKKLVSDDFLADTIEARQADLFEARTESNS"
     gene            592..2037
                     /gene="atpD"
                     /locus_tag="VFX21_13985"
     CDS             592..2037
                     /gene="atpD"
                     /locus_tag="VFX21_13985"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX5097131.1"
                     /translation="MTVTADTNTGTELKDGRVVAIAGPVVDVEFPPDALPEINTALEM
                     EIDLEGTTVVVTAEVAQQIGEGRVRAVCLKPTDGLTRGTSVRNTGAGITVPVGDGTLG
                     HVWNVIGEPLDIAKSEVSGVEDRWQIHREAPPFDQLEPKAQMFETGIKVIDLLEPYVQ
                     GGKIGLFGGAGVGKTVLIQEMINRVATQHGGVSVFAGVGERTREGTDLWLEMQESGVI
                     EKAALVYGQMDEPPGVRLRVALSALTMAEYFRDVKNQDVLLFVDNIFRFVQAGSEVST
                     LLGRMPSAVGYQPTLADEMGELQERITSTKGRSITSLQAVYVPADDYTDPAPFTTFTH
                     LDATTELAREIAALGIYPAVDPLASTSNILAPEIVGQRHYDVARRTQETLQRYKELQD
                     IIAILGLDELSEEDKITVARARKIQKFLSQPFFVGEVFTGLKGIFVPIDETVESFEAL
                     VNGELDNVPEQAFFNVGGVESVLAKAKTLEG"
     gene            2051..2467
                     /gene="atpC"
                     /locus_tag="VFX21_13990"
     CDS             2051..2467
                     /gene="atpC"
                     /locus_tag="VFX21_13990"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEX5097132.1"
                     /translation="MRVELVSPEQVLYEGEAEMVVVRTLGGGDIAFQPKHAQFLAALD
                     VWPAKVYRADGETMMFAVHGGFVEVSNDRVTILSDVAELAEQIDLERAQEALADAEAR
                     LQADPEDDHAIAQKKRAIARLDVCGVAAASASSAAH"
     gene            2541..>3475
                     /locus_tag="VFX21_13995"
     CDS             2541..>3475
                     /locus_tag="VFX21_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX5097133.1"
                     /translation="MDHDHAVGHDKHAGHDPEQFRRKFWLSLALTIPIVVTSEMVMDW
                     FGYSIDFRGMFLVGPVLGSVVFFYGGWPFLAGGAAEVRDRQPGMMLLISLAIVVAYVA
                     SLLSSFGWLDLEFWWELAALVTIMLFGHWQEMKAIGQARGALAALAELLPDDAERVSP
                     SGDVERVQVADLQLGDVVLVRPGARVPADGDVVDGEAEFDESMITGESRTVLRRPGDR
                     VIAGTVSTDTSVRVRVTAVGDDTALAGIQRLVAAAQASQSRAQVLADRFAAALFYVAV
                     ASGVVTFIVWALLGDVDSAVERTVTVLVIACPHALG"
BASE COUNT          558 a         1167 c         1182 g          568 t
ORIGIN      
        1 gactacgcga tcgacgccgc gttcagcggg ttctccgacc agccgaccta cgaggacgcc
       61 cgtccgatcg gtgaggcggc cacgcgcgcg ttcctcgccg aggagaccga cctggtcgag
      121 atcgtctaca cgcggttcgc gtcggcgggc atccaggtcg tcgagcgcca gaccctcgtg
      181 ccgatggtcc gccccgaggt cacgaccgac cacgaaggcg gtgtccccgc gtcgtacgag
      241 ttcgagccgt cgcccgattc catcctcgac ctcctgctcc cgcgctacgt cgactcgcgc
      301 gtgttcgcag cgttgctgaa cgcggcggcg tcggagcacg cgtcgcgcca acgcgccatg
      361 aagtcggcca ccgacaacgc aggcgatctc atcatcagcc tgagccgggt catgaaccgc
      421 gcccgccagg acgccatcac caccgagatc atggagatcg tgagcggtgc ggaggccctg
      481 cgcgggtcga agaagaagct cgtctccgac gacttcctcg ccgacaccat cgaagcccgc
      541 caggccgacc tgttcgaagc ccgcacggaa tccaactcgt aggagattgg gatgactgtc
      601 accgccgaca ccaacaccgg caccgagctg aaggacggtc gcgtcgtcgc gatcgccggc
      661 ccggtcgtcg acgtcgagtt cccgcccgac gcgctgcccg agatcaacac cgcgctcgag
      721 atggagatcg acctcgaggg cacgaccgtc gtcgtgaccg ccgaggtcgc gcagcagatc
      781 ggtgaaggcc gggtccgcgc cgtctgcctc aagcccaccg acggtctcac ccgtggcacc
      841 tcggtgcgca acaccggcgc cggcatcacc gtgcccgtcg gcgacggcac gctcggccac
      901 gtgtggaacg tcatcggcga accgctcgac atcgccaaga gcgaagtgag cggtgtcgag
      961 gaccgttggc agatccatcg cgaagcgccg ccgttcgacc agctcgagcc gaaggcgcag
     1021 atgttcgaga ccggcatcaa ggtgatcgac ctcctcgagc cgtacgtgca ggggggcaag
     1081 atcggcctgt tcggtggtgc gggcgtcggc aagacggtgc tcatccagga gatgatcaac
     1141 cgcgtcgcga cccagcacgg tggtgtgtcg gtgttcgccg gtgtcggcga gcgcacgcgt
     1201 gaaggcaccg acctctggct cgagatgcag gagtcgggtg tcatcgagaa ggccgccctc
     1261 gtgtacggcc agatggacga gccgccgggt gtgcggctgc gggtcgcgct gagtgcgctc
     1321 acgatggccg agtacttccg cgacgtgaag aaccaggacg tcttgttgtt cgtcgacaac
     1381 atcttccggt tcgtgcaagc gggttccgag gtgtcgacgc tgctcggccg catgccttcg
     1441 gccgtgggtt accagccgac gcttgccgac gagatgggcg agctgcagga gcgcatcacc
     1501 tcgaccaagg gtcgttcgat cacgtcgctg caggccgtct acgtgcccgc cgacgactac
     1561 accgaccccg cgccgttcac gacgttcacc cacctcgacg cgaccaccga gctcgcgcgt
     1621 gagatcgcgg ccctcggcat ctacccggcg gtcgacccgc tggcgtcgac gtccaacatc
     1681 ttggcgcccg agatcgtcgg tcagcgtcac tacgacgtcg ctcgtcgtac gcaggagacg
     1741 ctgcagcgct acaaggagct ccaggacatc atcgccatcc tcggtctcga cgagctgtcg
     1801 gaagaagaca agatcaccgt cgctcgggcc cgcaagatcc agaagttcct gagccagccg
     1861 ttcttcgtcg gtgaggtctt caccggcctg aagggcatct tcgtgcccat cgacgagacg
     1921 gtcgagagct tcgaagcgct cgtcaacggt gagctcgaca acgtgccgga gcaggcgttc
     1981 ttcaacgtcg gcggcgtcga gtcggtgctc gccaaggcca agacgctcga aggctgagag
     2041 aggcgaaccc gtgcgcgtcg agctggtgtc acccgaacaa gtgctctacg agggcgaagc
     2101 cgagatggtc gtcgtgcgca ccctcggggg cggcgacatc gcgttccagc ccaagcacgc
     2161 gcaattcctc gcggcgctcg acgtgtggcc cgcgaaggtc tacagggcag acggcgagac
     2221 catgatgttc gcggtgcacg gcggtttcgt cgaggtctcg aacgaccgcg tcacgattct
     2281 ctccgacgtc gccgagctcg cggagcagat cgacctcgag cgcgcgcagg aagcgctggc
     2341 cgacgcggaa gcgcggctgc aggccgaccc cgaggacgac cacgcgatcg cgcagaagaa
     2401 gcgggcgatc gcccgtctcg acgtgtgcgg cgtcgcggcg gcgtcggcgt catctgccgc
     2461 gcactgaccg cacggcatcc ctgccccgcg ggccggattt ctcttgcctc cgtgataccc
     2521 taggggggta tagtatcgac atggaccacg accacgcggt cgggcacgac aagcacgccg
     2581 gccacgatcc ggagcagttc cggcgcaagt tctggctgtc gctcgcgctg acgatcccga
     2641 tcgtcgtgac gtccgagatg gtcatggact ggttcggcta cagcatcgac ttccgcggca
     2701 tgttcctggt cggtccggtg ctgggcagcg tcgtcttctt ctacggcggt tggccgttcc
     2761 ttgccggtgg tgccgccgag gtccgagatc ggcagcccgg gatgatgttg ctcatctctc
     2821 tggcgatcgt cgtcgcgtac gtcgcgtcgc tgctctcgtc gttcggatgg ctcgatctcg
     2881 agttctggtg ggagttggcg gcgctggtga cgatcatgct gttcggtcac tggcaggaga
     2941 tgaaggcgat cggtcaggcg cggggtgcgc tggctgcgct cgcggaactg ttgcccgacg
     3001 acgcggaacg cgtgtcgccg agcggcgacg tggagcgcgt gcaggtcgcg gatctccaac
     3061 tgggagatgt cgtgctggtg cggccgggcg cgcgcgtccc cgcggacggc gacgtcgtag
     3121 acggagaggc cgagttcgac gagtcgatga tcacgggcga gtcgcgcacc gtgctccgcc
     3181 gcccgggtga tcgcgtcatc gcgggcaccg tgtcgaccga tacgtcggtg cgcgtgcggg
     3241 tcacggcggt cggcgacgac accgcgctgg ccggtatcca gcgcctcgtc gccgcggccc
     3301 aagcgagcca gagccgagcc caggtgctcg ccgaccggtt cgcggccgcg ctcttctacg
     3361 ttgccgtggc atccggcgtc gttacattca tcgtctgggc cctgctcggc gacgtcgaca
     3421 gtgcggtcga gcgcaccgtc accgtgctcg tgatcgcgtg cccgcacgcg ctcgg
//
LOCUS       DASUGA010000706         3263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1740392, whole genome shotgun sequence.
ACCESSION   DASUGA010000706 DASUGA010000000
VERSION     DASUGA010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740392"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VFX21_14000"
     CDS             complement(<1..308)
                     /locus_tag="VFX21_14000"
                     /inference="COORDINATES: protein motif:HMM:NF027503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5097134.1"
                     /translation="MPSPIVWRPDPELLRTSNVARFMAAEGIDSFAALTERSIDEPEW
                     FWDAVVRFLDIRFETPYREVLDVSDGAPWARWFRGGELNLAVTCVDRQADDPRRRDEP
                     A"
     gene            373..951
                     /gene="orn"
                     /locus_tag="VFX21_14005"
     CDS             373..951
                     /gene="orn"
                     /locus_tag="VFX21_14005"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020440693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEX5097135.1"
                     /translation="MNAEPGADALVWIDLEMTGLIAERDVIIEIACVVTTADLVAVDE
                     GVQFVVHQPPEALALMDDFVTNMHRRSGLTEEIAQSTIDLATAENEVLAYVKRHIPAA
                     GVAPLCGNSIGMDRRFIARYMPALDAHLHYRSIDVSSIKELCRRWYPDIYRKRPGKSE
                     QHRAQDDVLESIAELRFYRDELFTRPAADPAT"
     gene            complement(920..2239)
                     /locus_tag="VFX21_14010"
     CDS             complement(920..2239)
                     /locus_tag="VFX21_14010"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5097136.1"
                     /translation="MNPLDRIEAAQARSLERLRQDGPDRSLLVQLVEASCLLELAKLD
                     ATRLDPSTYLQLAVDVVAQMYPVVGCAATVLDGARTLEVHAGKMTDDVLRVPLVVREK
                     TIGVLVTGRLTSDLGSPADFLTRAADQIARGISIAFDAERLRRDAATATAARIASELS
                     PEDVVDGLEELALALASFPSVIAAELVLDHAAIGPPLHLRAGYWDDDGRAHSIGSSAI
                     DLGPTGRLAVRLRSNDEQSPDEGGVDAVLAGLAGSLERLLHTQRLVEDAETDMLSGLG
                     NRRRLERALAHALSRAERYGEHVAVMLIDLDRFKAVNDILGHETGDAVIVACACAMRE
                     RVRAYDETIRLGGDEFVVVAPVPDVLDALELADAVRLEIAQRCGAVLPPDWALSATIG
                     VALYPDAGTNQETLLRAADEALYRAKGDGRDTVAVAQLTSPDPPRGA"
     gene            complement(2236..3051)
                     /locus_tag="VFX21_14015"
     CDS             complement(2236..3051)
                     /locus_tag="VFX21_14015"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX5097137.1"
                     /translation="MASRYTQELLQLSASPAAAEQVLRLVGSSRSSAADLARVVETDP
                     ALSARVMRLANSPYYGASRRVSNTQHAIVMLGFDTVRGLAVSAACSLLDGRVDLGPDG
                     FWRHAVTTAAACATVAKKVGISSADAFSVGLLHDLGAVLLHRRDAQRFRDANHAPSMS
                     EIVAAEFAAFGTTHALAGSEALDAWGFPRPFVEAVAMHQHGVELASHALGRVLKVGEA
                     IALEHEPMPGYPTTYDLDRLLHSIRLRGEDFDEITREVDEQLERLADFLGIEP"
BASE COUNT          511 a         1133 c         1133 g          486 t
ORIGIN      
        1 gcgggctcgt cgcgccggcg tggatcgtcg gcttggcgat cgacgcacgt gaccgcgagg
       61 ttcagctcac cgccgcggaa ccagcgtgcc cacggcgctc cgtcggagac gtcgagcacc
      121 tctcgatacg gggtctcgaa gcggatgtcg aggaaccgga cgacggcatc ccagaaccac
      181 tccggctcgt cgatggagcg ctcggtcaat gccgcgaacg agtcgatgcc ctcggccgcc
      241 atgaatcgcg cgacgttgct cgtgcgcagc agctcggggt cgggtcgcca gacaatggga
      301 gaaggcaccg cttgcgaaat ctagtgatgc cgacctgtag cgtgcaccgc acacgagagg
      361 ggagctgtga cggtgaacgc cgagcccggc gcggatgctc tggtttggat cgatctcgag
      421 atgaccggtc tgatcgccga gcgtgacgtc atcatcgaga tcgcatgcgt cgtcacgact
      481 gccgatctgg tggccgttga cgagggtgtg cagttcgtag tgcaccagcc ccccgaagca
      541 ctggcactga tggacgactt cgtcaccaac atgcaccgcc gctcggggct gaccgaagag
      601 atcgcacagt cgaccatcga cctcgcgacg gccgagaacg aggtgttggc gtacgtgaag
      661 cgccacattc cggccgcggg cgtggcgccg ctgtgcggca acagcatcgg catggaccgc
      721 cgcttcatcg cgcgctacat gccggccctc gacgcgcacc tgcactaccg cagcatcgac
      781 gtgtcgtcga tcaaggagtt gtgccgccgc tggtatcccg acatctaccg gaagcggccc
      841 ggcaagagcg aacagcaccg cgcgcaggac gacgtcctcg agtccatcgc ggaactgcgc
      901 ttctatcgcg acgagttgtt cacgcgcccc gcggcggatc cggcgacgtg agctgcgcga
      961 ccgcgactgt gtcgcggccg tcgcccttgg cccgatagag cgcctcgtca gccgcgcgca
     1021 gcagtgtctc ttggttcgtg cccgcgtcgg gatacagcgc gacaccgatc gtcgcgctca
     1081 gtgcccagtc gggcggcagc actgcgccgc accgctgcgc gatctcgagc cgcaccgcgt
     1141 cggccagctc gagcgcgtcg aggacgtcgg gcaccggcgc cacgaccacg aactcgtcgc
     1201 caccgagacg gatcgtctcg tcgtacgccc gcacgcgttc gcgcatcgcg caggcgcacg
     1261 ccacgatcac cgcgtcgccg gtctcgtgcc cgaggatgtc gttcacggcc ttgaaccggt
     1321 cgaggtcgat gagcatcacc gcgacgtgct cgccgtaccg ctcggcccgg ctgagcgcgt
     1381 gcgccagggc ccgttcgagg cggcggcggt tcccgagccc gctgagcatg tcggtctccg
     1441 cgtcctcgac cagccgttgg gtgtgcagga gccgctccag cgagcccgcg agtcccgcca
     1501 gcacggcgtc gacaccgcct tcgtccggcg actgctcgtc gttcgaccgg agccggaccg
     1561 cgaggcgccc ggtcgggccg aggtcgatcg ccgagctgcc gatcgagtgg gcgcggccgt
     1621 cgtcgtccca gtagccggcg cggagatgga gcggcggccc gatcgccgcg tggtcgagca
     1681 cgagctcggc cgcgatcacc gacgggaacg acgccagcgc gagggcgagc tcctcgagtc
     1741 cgtcgacgac gtcttccggg ctcagttccg acgcgatccg cgcggcggtg gcagtcgcgg
     1801 cgtcacggcg gaggcgttcg gcgtcgaacg cgatgctgat accgcgcgcg atctggtccg
     1861 cggcgcgagt gaggaagtcg gcgggcgatc cgaggtcgct ggtgaggcgg ccggtgacga
     1921 gcaccccgat cgtcttttcg cgcacgacga gcggcacgcg caggacgtcg tctgtcatct
     1981 tgcccgcgtg cacttcgagc gtccgtgcgc cgtcgagtac cgtcgccgcg caacccacaa
     2041 cgggatacat ctgtgcgacg acgtcgaccg cgagctgaag ataggtcgac ggatcgaggc
     2101 gcgtcgcgtc gagcttcgcc agctccagca ggcacgacgc ctcgaccagc tgcacgagca
     2161 gcgagcgatc gggtccgtcc tgccgcagcc gttcgaggct gcgggcctgg gccgcttcga
     2221 tccggtcgag cgggttcacg gttcgatccc gagaaagtcc gcgagccgct ccagctgctc
     2281 gtcgacctcg cgcgtgatct cgtcgaagtc ctccccgcgg agacggatcg agtgcagcag
     2341 gcggtcgagg tcgtacgtcg tcggatatcc gggcatcggc tcgtgctcga gcgcgatcgc
     2401 ctctccgacc ttgagcacgc gtccgagcgc gtgcgacgcc aactcgacac cgtgttggtg
     2461 catcgcgacg gcctcgacga agggacgcgg aaagccccac gcgtcgagcg cctccgaacc
     2521 ggcgagcgcg tgcgtcgtcc cgaacgcggc gaactcggcc gcgacgatct ccgacatcga
     2581 cggcgcgtgg ttcgcgtccc ggaatcgctg cgcatcgcgc cggtgcagca acaccgcgcc
     2641 cagatcgtgc aacagcccga cggagaaggc atcggcgctc gagatgccga ccttcttcgc
     2701 gaccgttgca cacgcggcgg cggtcgtgac cgcgtggcgc cagaagccgt cgggaccgag
     2761 gtcgaccctt ccgtcgagca ggctgcacgc ggcactgacc gccaggccgc gcaccgtgtc
     2821 gaagccgagc atgacgatcg cgtgttgcgt gttcgagacc cgccgggacg cgccgtagta
     2881 gggactgttc gcgaggcgca tgacccgcgc cgacagcgcg gggtccgtct cgacgactct
     2941 cgccaagtcg gccgccgacg accgcgagct gccgacgagg cggagcactt gctcggcggc
     3001 ggccggcgac gcggagagct gcaggagctc ttgtgtgtac cggctggcca ttcgcagagt
     3061 tgtcggcgcg ccgcaaccga agattgatcg ggcgtttttt cggcagcgat ttccgctgca
     3121 gaaaaacgcc cggctgatca ggccagcaac gcttcgaggg caccctggag ggtgacgacc
     3181 gagcggtcgc gcgcgctgtg gcccatgagg ccgatgcgcc aacccttgcc ggccagttcg
     3241 ccgaggccgc cgcccacctc gat
//
LOCUS       DASUGA010000707         2088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_490213, whole genome shotgun sequence.
ACCESSION   DASUGA010000707 DASUGA010000000
VERSION     DASUGA010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490213"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..784
                     /locus_tag="VFX21_14020"
     CDS             92..784
                     /locus_tag="VFX21_14020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HEX5097138.1"
                     /translation="MSDAEQSSEKKEPPKPPTRLVLVRHAVTAHTGPILSGRMPGIDL
                     SEKGVGQADATAQRLAKLPVAVVYASPIERTTQTAQRIASHHGLDVIPLVGVIEAEYG
                     DWTGGKIADLAKTDEWKVVQVAPSRAKFPNGETITAMQARMVAALDDLVAKHPHETVV
                     VVSHADPIKSAIAHYTGMHLDLFQRVHVSPASVTVFDFHTYGVMMVKCNDTGGLDDLL
                     PEPEAPATEASA"
     gene            790..1305
                     /locus_tag="VFX21_14025"
     CDS             790..1305
                     /locus_tag="VFX21_14025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HEX5097139.1"
                     /translation="MIEFDPVDDIGAGAFGQPGERTFVIQARKGGAVLSVLVEKEQVR
                     MLAEEANQFLDRIAEEDPEDPPTSVEVRGGSVTEDEPLFRARLIGIGYDTDRHLVLIE
                     LREEATDDEEESPVELDDADGRVARLYATRAQIRVMARNGVAAVESGRPKCQLCDFPM
                     DPAGHICPRWN"
     gene            1322..2026
                     /locus_tag="VFX21_14030"
     CDS             1322..2026
                     /locus_tag="VFX21_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HEX5097140.1"
                     /translation="MTLPDARARQLLREGDPDVLGRMPWSSNATFLVNLTLDGDELLA
                     IYKPQRGERPLWDFPRGTLCHREVAAFQVSEALGWKLVPDTVLRNGPHGIGMMQRFVE
                     HDPDEHYFTLLEQHADDFRRMAAFDIVINNTDRKGGHCLRALEDSSIFGIDHGVAFHM
                     QWKLRTVIWDFGGEPIQPDVCNDLHRFVECIRDLDLDLDRFELDALRARTEHLLATGE
                     YPEPDRDYHSYPWPTI"
BASE COUNT          392 a          676 c          682 g          338 t
ORIGIN      
        1 gaggatcgtg tcgggcgggc ccgacggcga gaggttgacg ccggtcaccg gggggagcgt
       61 agaggatcga gggcggtaac ttggcgtcca gatgtccgac gccgagcagt cgagcgaaaa
      121 gaaagaaccg cccaagccgc cgacgcgctt ggtgctcgtg cgccacgcgg tcaccgcgca
      181 cactggtccg attctttccg gacgcatgcc cggcatcgac ttgtccgaga aaggcgtcgg
      241 gcaagccgac gcgaccgcgc agcggctggc gaagctcccc gtagcagtgg tgtacgcgag
      301 tcccatcgaa cggaccaccc agaccgcgca acgcatcgcg tcgcaccacg gccttgatgt
      361 gatcccactc gttggtgtga tcgaggcgga atacggcgac tggaccggcg gaaagatcgc
      421 cgatctggcg aagaccgacg aatggaaggt cgtgcaggtc gcgccgtcgc gggcgaagtt
      481 cccgaacgga gagacgatta ccgcaatgca ggcgcgcatg gtcgccgcgc tcgacgatct
      541 ggtcgccaag catccgcacg agaccgtcgt cgtcgtgagc cacgccgacc cgatcaagtc
      601 ggcgatcgcg cattacacgg gcatgcatct cgatctgttc cagcgggtac acgtgtcgcc
      661 ggcgtcggtc acggtgttcg acttccacac ctacggcgtg atgatggtga agtgcaacga
      721 caccggcggc ctcgacgacc tcctgcccga accggaagcc ccggcaacgg aggcaagcgc
      781 gtgaccgaca tgattgagtt cgatccggtc gacgacatcg gggccggcgc gttcgggcaa
      841 ccgggtgagc gcacgtttgt catccaggct cgcaagggcg gcgccgtgtt gtcggtgctc
      901 gtcgaaaaag aacaggttcg catgctcgcc gaagaggcga accagttcct cgatcgcatc
      961 gccgaagagg accccgagga tccgcccacg tcggtcgagg tgcgcggtgg ttcggtcacc
     1021 gaagacgagc ccctgtttcg cgcccggctc atcggcatcg gctacgacac cgaccgccat
     1081 ctcgtgctga tcgaactgcg cgaggaagcg acggacgacg aagaggagag tcccgtcgaa
     1141 ctcgacgacg ctgacggccg ggtggcgcgc ttgtacgcga cgcgcgcgca gatccgggtg
     1201 atggcgcgca acggtgttgc cgcggtcgag tccgggcggc ccaagtgcca gctctgcgac
     1261 ttcccgatgg atcccgcagg gcacatttgc ccgcgctgga attgatttgg ggcactgagc
     1321 ggtgacactg cccgacgcgc gcgcccgtca actgctgcgc gagggcgacc ccgacgtctt
     1381 gggtcgtatg ccgtggtcgt cgaacgcgac cttcctggtc aacctcacgc tcgatggcga
     1441 cgaactcctc gcgatctaca agccgcagcg aggcgagcgc ccgctctggg attttccgcg
     1501 cggaacgttg tgccaccgtg aagtcgcggc gtttcaagtg tcggaagcgc tcggctggaa
     1561 gctcgttccc gacacggtcc tgcgcaacgg accgcacggc atcggcatga tgcaacgctt
     1621 cgtcgaacac gatcccgacg agcattactt cacgctgctc gagcagcacg ccgacgactt
     1681 ccggcgcatg gcagccttcg acatcgtcat caacaacacc gaccgcaagg gcggacactg
     1741 cctgcgcgcg ctcgaagaca gctcgatctt cggcatcgac catggcgtcg cgtttcacat
     1801 gcagtggaag ctgcgtaccg ttatctggga cttcggcggc gaaccgatcc agcctgacgt
     1861 ctgcaacgac ctgcaccgtt tcgtcgaatg cattcgtgac ctcgacctcg acctcgatcg
     1921 attcgaactt gatgcgttgc gggcacgcac cgagcacctg ctggcgacgg gggagtaccc
     1981 ggaacccgat cgcgactacc acagctaccc ctggcccacg atctagcgca tacctgcgtc
     2041 cgtttggcag atggggtatg ccaaacggac gcaggtatgt cagcgggc
//
LOCUS       DASUGA010000708         4004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1601952, whole genome shotgun sequence.
ACCESSION   DASUGA010000708 DASUGA010000000
VERSION     DASUGA010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601952"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFX21_14035"
     CDS             <1..528
                     /locus_tag="VFX21_14035"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5097141.1"
                     /translation="SVNVSPRQFSHPGFVERLERIIRRTGVDPQSLLLEITESVLVTG
                     ADQGLALLENIKRLGLRLAIDDFGTGYSALAYLRQFPVDVVKIDRVFMDELGRLAVDT
                     TVVAAIIRLAQVLGQQVIAEGAETAQQMAALYELGCDFVQGFYFAPPLAQAQAEMLVR
                     GRRDLASVRTALQRA"
     gene            complement(536..817)
                     /locus_tag="VFX21_14040"
     CDS             complement(536..817)
                     /locus_tag="VFX21_14040"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX5097142.1"
                     /translation="MGIFWWIVVGFVAGAFARGVTKGAPDAGCIFTTVIGIIGALIGG
                     ALFNAVGDHDLNDSILGSMFVAFIGACVLLLIVGLFTKGRYGSDSSRWN"
     gene            complement(999..2801)
                     /locus_tag="VFX21_14045"
     CDS             complement(999..2801)
                     /locus_tag="VFX21_14045"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015610995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEX5097143.1"
                     /translation="MTATPPTTNRKLTEWVDHWAAILEPDQVHWCDGSEAEYAALCTQ
                     LVASGTFTPLDPAKRPNSFYAASDPGDVARVEGRTFICSANEADAGPTNNWRDPDEMR
                     AQMLELYRGAMHGRTLYVVPFSMGPLGSPIAHIGVELTDSAYVAASMRTMTRMGKGAL
                     DVLGENGEFVPCIHSVGMPLENGAPDVPWPCNNEQKFIVHYPETREIQSYGSGYGGNA
                     LLGKKCFALRIASVMARDEGWMAEHMLILKLTSPANEVSYIAAAFPSACGKTNLAMLV
                     PTIPGWKAETIGDDICWMKFGSDGCLYAINPEYGMFGVAPGTGLDTNANAIAALTRNT
                     VFTNVALTDDGDVWWEGLTDDKPAHLTDWKGNSWTPDSDSPAAHPNSRFTAPLSQVPC
                     VAPEWEDPKGVPISAILFGGRRRSTVPLVTEAYNWQHGVFLGSIMGSETTAAATGAVG
                     ALRRDPFAMLPFCGYNMGDYFGHWLDIGAGADADKLPRLFYVNWFRKDDDGKFLWPGY
                     GDNSRVLAWVCERAAGRGAAKDTAIGRLPADGALPLDGLGLPAAAVAELLDVDADEWR
                     AELPLIEEHYAVFGDRLPQALHNELDALAKRLAE"
     gene            2913..3149
                     /locus_tag="VFX21_14050"
     CDS             2913..3149
                     /locus_tag="VFX21_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX5097144.1"
                     /translation="MTVSAYILIQTEVGKASAVVQAVRPLPGVVQADDVTGPYDVILR
                     AEADSIDDLGRMVVSQVQLVDGITRTLTCPVVHL"
     gene            complement(3160..>4004)
                     /locus_tag="VFX21_14055"
     CDS             complement(3160..>4004)
                     /locus_tag="VFX21_14055"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEX5097145.1"
                     /translation="GEVVPAPNPSTSTAVTPLGQVDVVFPLLHGPYGEDGTVQGLLEL
                     AGLPYVGSGVLGSAIGMDKYAMKRMLEAAGIAVAPYTPLREGDRLDAIDVAFPCFVKP
                     ANMGSSVGISKAHNRAELEAAVALAFEYDEWILVEDFVPGREIELAVLGDRSIDVSVP
                     GEVVPGAEFYDYADKYENGTAQLLVPAPLTAAEVERAQRLAALTFDACRCEGMARVDL
                     FLCDDGSFVVNEVNTIPGFTPISMYPRLWEASGVPYSQLLDRLIAFAISRHDRRHKRA
                     GKQR"
BASE COUNT          611 a         1377 c         1386 g          630 t
ORIGIN      
        1 tcggtgaacg tcagcccccg gcagttctcg cacccgggct tcgtcgagcg gctggagcgc
       61 atcatccgcc gcaccggtgt cgacccgcag tcgctgctgc tggagatcac cgagagcgtc
      121 ctggtcaccg gcgccgacca aggcctcgcg ctgctcgaga acatcaagcg gcttgggcta
      181 cggctcgcga tcgacgactt cggcaccggc tactcggcgc tcgcgtatct ccggcagttc
      241 cccgtcgacg tcgtgaagat cgaccgcgtc ttcatggacg agctcggacg cctcgcggtc
      301 gacacgaccg tggtcgccgc gatcatccgc ctcgcgcagg tgctcggcca gcaggtcatc
      361 gccgagggcg ccgagacggc gcagcagatg gccgcgctgt acgagctcgg ctgcgacttc
      421 gtgcagggct tctacttcgc gccgccgctc gcgcaggcgc aggccgagat gttggtccgt
      481 ggccgccgcg acctcgccag cgtccgcacc gcgctccagc gcgcctagtc ggtacctagt
      541 tccagcgtga gctgtcggat ccgtatcggc ccttggtgaa caggccgacg atcagcagca
      601 gtacgcacgc gccaatgaac gcgacgaaca tcgagcccag gatcgagtcg ttgaggtcgt
      661 ggtcgccgac tgcgttgaac aacgcgccgc cgatcagcgc gccgatgatg ccgatcaccg
      721 tcgtgaagat gcacccggca tccggcgcgc ccttcgtgac gcctcgcgcg aacgcaccgg
      781 cgacgaaccc gacgacgatc caccagaaga tgcccacgcc cacaacggta gcgaggtcgc
      841 ggcgccgagc gctcgcgtac ctgcgtgact gaaccacaca agcggtgggt tgagccacgc
      901 aagaacgaag cgcggacgtc gcccgcgcga accggcgcct ttgaaccacc gggacggtgg
      961 gtgagcgacg caagaacgag gcgcggacgt cggcgtgact actcggcgag gcgtttggcg
     1021 agcgcgtcga gctcgttgtg gagcgcctgc gggagccggt cgccgaagac ggcgtagtgc
     1081 tcctcgatca acggcagttc cgcgcgccat tcgtcggcgt cgacgtcgag gagctcggcg
     1141 acggcggcgg ccggcaggcc gagaccgtcg agcggcagcg ccccgtccgc gggcaggcgg
     1201 ccgatcgcgg tgtccttggc cgcaccgcgc cccgcggccc gctcgcacac ccacgcgagc
     1261 acccggctgt tgtcgccgta gcccggccag aggaacttgc cgtcgtcgtc cttgcggaac
     1321 cagttcacgt agaagagacg cggcagcttg tccgcgtcgg cgccggcacc gatgtcgagc
     1381 cagtgcccga agtagtcgcc catgttgtat ccgcagaacg gcaacatcgc gaacgggtcg
     1441 cggcgcagcg cgccgaccgc gccggtcgcc gcggccgtcg tctccgaacc catgatcgaa
     1501 ccgaggaaca cgccgtgctg ccagttgtac gcctcggtca cgagcggcac ggtgctgcgg
     1561 cggcggccgc cgaagaggat cgccgagatc ggtacgccct tcgggtcctc ccactcgggc
     1621 gcgacgcacg gcacttgcga cagcggcgcg gtgaagcggc tgttcgggtg cgcggccggt
     1681 gagtcggagt cgggcgtcca gctgttgccc ttccagtcgg tgaggtgcgc gggcttgtcg
     1741 tcggtgagcc cttcccacca gacgtcgccg tcgtcggtga gcgcgacgtt cgtgaacacc
     1801 gtgttgcggg tgagcgcggc gatcgcgttc gcgttcgtgt cgagaccggt gcccggcgcg
     1861 acaccgaaca tgccgtactc agggttgatc gcgtagaggc acccgtcgga tccgaacttc
     1921 atccagcaga tgtcgtcgcc gatcgtctcg gccttccagc cggggatcgt cggcaccagc
     1981 atcgcgaggt tcgtcttgcc gcacgcgctc gggaacgcgg ccgcgatgta ggacacctcg
     2041 ttggccgggc tcgtcagctt gaggatgagc atgtgctcgg ccatccagcc ctcgtcgcgc
     2101 gccatcaccg acgcgatgcg caacgcgaag cacttcttgc cgagcagcgc gttgccgccg
     2161 taacccgatc cgtacgactg gatctcgcgc gtctccgggt agtgcacgat gaacttctgc
     2221 tcgttgttgc acggccacgg cacgtcgggc gcaccgttct cgagcggcat accgaccgag
     2281 tggatgcacg ggacgaactc gccgttctcg ccgagcacgt cgagcgcgcc cttgcccata
     2341 cgcgtcatcg tgcgcatgct cgccgcgacg tacgcggagt cggtgagctc gacaccgatg
     2401 tgcgcgatcg gtgagccgag cgggcccatc gagaacggga cgacgtacaa cgtgcgcccg
     2461 tgcatcgcgc cccggtagag ctcgagcatc tgcgcccgca tctcgtctgg gtcccgccag
     2521 ttgttcgtcg ggcccgcgtc ggcttcgttc gcgctgcaga tgaacgtgcg gccctcgacg
     2581 cgggcgacgt cgccggggtc gctcgccgcg tagaagctgt tcgggcgctt ggccgggtcg
     2641 agcggcgtga acgtgccgct ggcgacgagc tgcgtacaga gtgccgcgta ttccgcctcg
     2701 gaaccgtcgc accagtgcac ctggtcgggt tcgaggatcg ccgcccagtg gtcgacccac
     2761 tcggtgagct tgcggttcgt tgtcggcggt gtcgcggtca ttggccggct cgtcctccac
     2821 acgcaaacgg gtacgtgatg gtatgcctca ccccggtacc ccggccgaac cgtgcgcagt
     2881 acgatccgtc cgccgagggc aggaggtcgc ttgtgaccgt cagcgcatac atcctcatcc
     2941 agaccgaagt cggcaaggcg tccgctgtcg tccaggcggt tcgcccgctt ccgggcgttg
     3001 tgcaggccga cgacgtgacc ggcccctacg acgtgatcct gcgggccgaa gccgactcga
     3061 tcgacgatct cgggcgcatg gtcgtgtcgc aggtccagtt ggtggacggg atcacccgca
     3121 ccctgacctg ccccgtcgtt cacctgtagc cgcggcgcct cagcgctgtt tgccggcgcg
     3181 cttgtggcgg cggtcgtggc gggagatcgc gaacgcgatc aggcggtcga ggagctgcga
     3241 gtacgggacg cccgacgcct cccacaggcg cgggtacatc gagatcggcg tgaagccggg
     3301 gatggtgttc acctcgttga cgacgaacga accgtcgtcg catagaaaca ggtcgacgcg
     3361 cgccatgccc tcgcagcggc atgcgtcgaa cgtgagcgcg gcgaggcgtt gcgcgcgctc
     3421 gacttcggcg gcggtgagcg gcgcgggcac gagcagctgc gcggtgccgt tctcgtactt
     3481 gtccgcgtag tcgtagaact ccgcgccggg gacgacttcg ccgggcaccg agacgtcgat
     3541 cgaacggtcg ccgagcactg cgagctcgat ctcgcggccg gggacgaagt cttcgaccag
     3601 gatccactcg tcgtactcga acgcgagcgc gaccgcggct tcgagctcgg cgcggttgtg
     3661 cgccttcgag atgccgacgg acgagcccat gttcgcgggc ttgacgaagc acgggaacgc
     3721 gacgtcgatc gcgtcgagac ggtcaccctc gcgcagcggc gtgtacggcg cgacggcaat
     3781 gcccgcggcc tcgagcatgc gcttcatcgc gtacttgtcc ataccgatcg cggacccgag
     3841 cacgccggag ccgacgtacg gcagcccggc cagctcgagc aggccctgca cggtgccgtc
     3901 ctcgccgtag gggccgtgca gcaacggaaa cacgacgtcg acctgaccga ggggcgtcac
     3961 cgcggtgctc gtgctcgggt tcggcgcggg cacgacctcg cccg
//
LOCUS       DASUGA010000709         7017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1252205, whole genome shotgun sequence.
ACCESSION   DASUGA010000709 DASUGA010000000
VERSION     DASUGA010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7017
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252205"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..802)
                     /locus_tag="VFX21_14060"
     CDS             complement(77..802)
                     /locus_tag="VFX21_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX5097146.1"
                     /translation="MRKPWKWIVGAVVVVAIALVVGPWVYINFIQEDAPPELQLTDAT
                     ANTTDATRAPGSTSATTAPETAGATGIDGTWNVGEGSTAGYRAKEVLFGQDADAAGRT
                     DQVTGSMEISGTTVTTGSFEVDMASVESDQSNRDDAYRGRIMNVDQFPTSTFELTTPI
                     ELGSIPEEGVAIHPQATGDITIHGVTKTVTIDLDAQLIDGKIEVVGHLPVKWSDFDID
                     DPSGGPATVTDNGQMEFKLVLSR"
     gene            complement(836..1564)
                     /locus_tag="VFX21_14065"
     CDS             complement(836..1564)
                     /locus_tag="VFX21_14065"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5097147.1"
                     /translation="MAALSLFAGLAGGLIAADNSHDTTRAAAAFTGSPTDIAAVLARV
                     EPSVVSIESRIGTRRGPFAATGTAAGSGIVIGTNGTILTNAHVIDGANAITVTVAGES
                     KARTATVIASDAGRDMALLQVDDTDGLVAAPLGDSAGLAVGDSVVAIGNALALEGGPT
                     VTSGIVSALDRSLDTETGSLSGLMQTDAAISSGNSGGPLVNAAGDVIGMNTAVATSNG
                     SVSASNIGFAISIDTIRSFLAQVS"
     gene            complement(1779..3206)
                     /locus_tag="VFX21_14070"
     CDS             complement(1779..3206)
                     /locus_tag="VFX21_14070"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5097148.1"
                     /translation="MDRRDFIRAGAVLGVAGTLGSRALPAWGHTTPPLRQESILQGHP
                     GDSGVDTVVVVMMENRSFDSYLGWLAQDKAYITNGRSRYGATFKVNGARDQQYPDPAT
                     GQLVSTYLRSQTDPTNWRGCTLNDPGHGWTAGRAERDGGFLATGSGNDILALSYFNPR
                     DLPVYRELARRFTTCDQWHASLLGPTFPNREYLLSAQSGGHKSNDSEPGGFKWETIVD
                     RLAAANVSVAEYYSDLPVFALWGSRLLPYLRPVTNFFADAAAGKLPQVCFVDPKFVGP
                     ERTDDHPHGDPRAAQRFVRDAFAAFANSPHWQRGLFILTYDEWGGFFDHVRPPLMNDN
                     RASSVDTENFAQCGFRVPTILCSPRSLPGSVDHTLYDHTAVLRFLEWRFLGAPPRGHR
                     FHGKTWWLTKRDRYSANLGDSLSRTYFDPDPGFDVNVKVAEPQDPCVGQSLAPQILSQ
                     GDEDGFEDLYNSGYFDSVGAKTYVD"
     gene            complement(3281..3739)
                     /locus_tag="VFX21_14075"
     CDS             complement(3281..3739)
                     /locus_tag="VFX21_14075"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX5097149.1"
                     /translation="MGHVQITTPLGPFLAVVEDGMVTESRFVAGGRARNDDPEVHELL
                     EAYFAGDVGALDKIDVNAKGTTFQHKVWDALREIPVGETWSYGELAGHIGAPGASRAV
                     GTANASNPVGLIVPCHRVVRADGSVGGYGFGPERKRWLLEHEGARLPSLL"
     gene            3830..5215
                     /locus_tag="VFX21_14080"
     CDS             3830..5215
                     /locus_tag="VFX21_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HEX5097150.1"
                     /translation="MATSSFTAVVTTGIYCRDGCPATPLRRNTLPYEYAAAAEAAGFR
                     PCLRCRPDRVPDPGWVDAPELVCRALRLITDGYLDGATEDVLAARLGVSARHLRRLFD
                     EHVGATPAEVARSRRAHFARRILDDTELSIAEVSRAAGFTTVRHMNRVMKQVFQFTPQ
                     ELRAKRRDPRRLVADGGLELRLPYRAPLAWDTMIRYLAPRAIPGVEAVDVDAGVYRRT
                     IVFGDAPGVIEVRDSDERTHVLLRVHLSRFDGLVHLVQQVRRQFDLDADPEAIDAFLA
                     RDPKLRPLVRRHRGLRVPGAYDPVELGARAILGQQVTVAGATRLAGRVVEAHGTPVPG
                     LEALGLTHTFPSAAVLGDAPLERLGLTAARARAVREFAAASPKLRFDGGMPLAELVGV
                     LVDLPGIGAWTAHYIAMRAAGERDAFPAGDLGLRKALDAPSAHAVGEASNRWRPWRAY
                     AAMQLWCSTSA"
     gene            5452..6405
                     /locus_tag="VFX21_14085"
     CDS             5452..6405
                     /locus_tag="VFX21_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5097151.1"
                     /translation="MPAKVNKSRSVDRDYEDLVRLYLEDVGRHDLLTKDDEARLAQAI
                     EAGVAARAELDAGKRLTPTQRRNLRRDVRRGEEAHRQFVNSNLRLVVSIAKKYQSSGL
                     PLLDIVQEGNLGLIHAVDKFDWRKGFKFSTYATWWIRQAIQRGIANSARVIRLPVHAG
                     DMLTALLKLRAQLEGSLGRTPTLAELATEAELPLDKVVEVLRYAVDTVSLDEPVRDDG
                     DAELGDFVEDRNATAPFDHAATSLLPGEVAKVLAVLDDRERTILTLRFGLDGGGECTL
                     EEVAEHFGLTRERIRQIEARAMSKLRHPSADVGARELLEAV"
     gene            6487..>7017
                     /locus_tag="VFX21_14090"
     CDS             6487..>7017
                     /locus_tag="VFX21_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097152.1"
                     /translation="MTGAFRIARNPDPDSTLPYVLSVPIGATPLVLKAKDTWPRTAKV
                     YCHRGAWPEDAEIVEETPVRSCVRRGVAIDLVLDRARESRSQFVITTLKGGREGIFWQ
                     SARTNRKTRPGMRLPTKRSAAGVLEVVVDTRERYAWKFGDQQVVIERRALRVGDYAVE
                     FDGEVVGVVERKKLAEL"
BASE COUNT         1055 a         2399 c         2417 g         1146 t
ORIGIN      
        1 cacgttcgca gcgtcgcgcc cgaacttggg aacaacctga gagtcgaccg ggagctcggt
       61 cagagccggc cgggagctac ctgctcaaca cgagcttgaa ctccatctgg ccgttgtcgg
      121 tcaccgtggc cgggccgccg ctcgggtcgt cgatgtcgaa gtcggaccac ttgaccggca
      181 ggtgcccgac gacctcgatc ttgccgtcga tgagctgcgc gtcgaggtcg atcgtgacgg
      241 tcttggtgac gccgtggatc gtgatgtcac cggtggcctg cgggtggatc gccacacctt
      301 cttcgggaat cgagccgagc tcgatcggtg tggtgagctc gaacgtcgac gtcgggaatt
      361 ggtcgacgtt catgatgcgc ccgcggtagg cgtcgtcgcg gttgctctga tcgctctcga
      421 cggatgccat gtcgacctcg aacgagccgg tcgtcaccgt cgtgccgctg atctccatcg
      481 accccgtgac ctgatcggtg cgacccgcgg catcggcgtc ttgaccgaac agcacttcct
      541 tggcgcggta tcccgcggtg cttccttcgc cgacgttcca cgtaccgtcg atgccggtcg
      601 cgccggcggt ctcgggtgct gtcgtcgcgc tcgtggaacc gggtgctcgc gtcgcgtccg
      661 tcgtgttcgc cgtcgcgtcc gtcaactgca actcgggtgg cgcgtcctct tggatgaagt
      721 tgatgtagac ccacggcccg acaacgagcg cgatggcgac gacaacgacc gcaccgacga
      781 tccacttcca gggcttgcgc atggttcgtc cttacgtcgg ctcacgccgg atggttcacg
      841 agacctgtgc gaggaaagac cgaatcgtat cgatcgagat cgcgaatccg atgttggatg
      901 cgctgacgga accgttgctc gtcgcaaccg cggtgttcat cccgatgaca tcgccggcgg
      961 cgttcacgag cggtccaccc gagttgccgg agctgatggc ggcgtcggtc tgcatcagac
     1021 ctgacagcga acccgtctcg gtgtcgagcg agcggtcgag cgccgagacg atgcccgacg
     1081 tcaccgtcgg tccgccctcc agcgcgagcg cgttgccgat cgcaacgacg ctgtcgccga
     1141 ccgccaagcc ggcactgtcg ccgagtggtg cggcgacgag accgtccgtg tcgtcgactt
     1201 gcaacagcgc catgtcgcgg ccggcgtcgc tcgcgatcac ggtcgcggtg cgcgccttcg
     1261 actcgccggc gacggtgacc gtgatcgcgt tcgcgccgtc gatgacgtgc gcgttggtga
     1321 ggatcgtgcc gttggtcccg atgacgatgc cgctgcccgc agcggtgccc gtcgccgcga
     1381 acggtccgcg cctcgtgccg atgcgggatt cgatcgagac gacggacggt tcgacgcgcg
     1441 cgagcaccgc cgcgatgtcg gtcgggctgc cggtgaacgc cgccgcggcg cgggtggtgt
     1501 cgtgggagtt gtccgcggcg atcagtccac cggccagccc ggcgaacagg gagagcgccg
     1561 ccacgcccgc accgaccgca atgaagcggc cgcgggggcg tgcggggggc ggcggactcc
     1621 cggcctccgt caccccgggg gagaaaggcg gcggagggtc gagccacggc ggaccgctca
     1681 ggtcgatgtc cggtggcctc gactgcgtct gcatgtcgcg gatgatgacg acggggcctg
     1741 cgaacgctcc ccgagctgcc cgtgagcttc ctgtgagatc agtccacgta ggtcttggcg
     1801 ccgacgctgt cgaagtaacc cgagttgtag aggtcctcga agccgtcctc gtcgccctgc
     1861 gacaagatct gcggcgcgag cgattggccg acgcacgggt cctggggttc ggccaccttg
     1921 acgttcacgt cgaatccggg gtccggatcg aaatacgtgc gcgacaagct gtcgccgagg
     1981 ttcgccgagt atcggtcacg cttggtcaac caccacgtct ttccgtggaa gcggtgcccg
     2041 cgcggcggcg caccgaggaa gcgccactcg aggaaccgca agacggccgt gtgatcgtag
     2101 agcgtgtggt cgaccgagcc cggcagcgag cgcggggagc acaagatcgt cggaacgcgg
     2161 aaaccgcact gtgcgaagtt ctccgtgtcg accgagctgg ccctgttgtc gttcatcaac
     2221 ggcgggcgca cgtggtcgaa gaagccaccc cactcgtcat aggtgaggat gaacagtccc
     2281 ctctgccagt gcggcgagtt ggcgaacgcg gcaaacgcgt cgcgcacgaa tcgctgtgcc
     2341 gcgcgcggat cgccgtgcgg gtggtcgtcc gtgcgttcgg gcccgacgaa ctttggatcg
     2401 acgaagcaca cctgcggcaa cttcccggcg gcggcatcgg caaagaagtt cgtgaccgga
     2461 cggaggtagg gaagcagacg actgccccag agcgcgaaga ccggcaagtc cgagtagtac
     2521 tccgcgaccg agacgttcgc ggcggcgagc cggtcgacga tcgtctccca tttgaagccg
     2581 ccgggctcgc tgtcgttgct cttgtgcccg cccgactgtg ccgacaggag gtactcgcgg
     2641 ttggggaacg tcggcccgag cagcgacgcg tgccattggt cgcatgtcgt gaagcgccgc
     2701 gcgagctcgc gatacacggg gaggtcacgc gggttgaaat agctgagcgc aaggatgtca
     2761 ttgccgctgc cggtcgcgag gaagccgccg tcgcgttcgg cccgtccggc ggtccacccg
     2821 tgaccgggat cattcagtgt gcaaccgcgc cagttcgtcg ggtcggtctg cgaacgcagg
     2881 tatgtcgaga cgagttgccc cgtcgcgggg tcgggatact gctggtcgcg ggccccgttg
     2941 accttgaacg tcgcgccgta cctggatcga ccgttcgtga tgtacgcctt gtcttgcgcc
     3001 agccaaccca ggtagctgtc gaaggagcgg ttctccatca tcacgacgac gacggtgtcg
     3061 accccactgt cgcccgggtg tccctggagg atcgactcct ggcgcaacgg cggcgtggtg
     3121 tgcccccacg ccggcagcgc gcgcgatccg agcgtgcccg ccacacccag cactgcaccg
     3181 gcccggatga agtccctgcg atccatgcgc cggatcgtgt cgcggcgcgg cgcgtctgcg
     3241 aaagcgtcgc cggatcacgc gtcgggggtt ttgtgacgtt tcataagagc gaaggcaacc
     3301 gcgcgccttc gtgctccagc aaccagcgct tgcgctcggg cccgaagccg tacccgccga
     3361 ccgaaccgtc ggcgcgcacg acccggtggc acggcacgat caaccccacc ggattcgacg
     3421 cattcgcggt gccgaccgca cgcgacgcgc ccggtgcacc gatgtgcccg gcgagctcgc
     3481 cgtagctcca cgtctcgccg acggggatct cgcgcaacgc gtcccagacc ttgtgctgga
     3541 acgtcgtgcc cttcgcgttc acgtcgatct tgtcgagcgc gccgacgtcg ccggcgaagt
     3601 acgcctcgag cagttcgtgc acctcggggt cgtcgttgcg ggcgcgcccg ccggcgacga
     3661 agcgcgactc ggtgaccatg ccgtcctcga cgacggcgag gaacggaccg agcggagtgg
     3721 tgatctgtac gtgtcccatg gttgcattct gcgaccaccg cgctggaccg atccggacac
     3781 gccctagggt tgtccggatc cgtctcctcg gggccgcgta gaaggggtta tggcaacttc
     3841 gtcgttcacc gctgtggtca cgaccggcat ctactgccgt gacggctgcc ccgcgacccc
     3901 gctgcgccgg aacacgcttc cctacgagta cgcggcagcc gccgaagccg cgggcttccg
     3961 gccctgcctg cgctgccgtc ccgaccgtgt gcccgatccg gggtgggtcg acgcgcccga
     4021 gctcgtgtgc cgcgcgctgc gcttgatcac cgacggctat ctcgacggtg caaccgaaga
     4081 cgtgctcgcc gcgcggctcg gtgtgagcgc ccgtcacctg cgccggctgt tcgacgagca
     4141 cgtcggcgcg acaccggcgg aagtcgcccg ctcccgccgg gcgcatttcg cacgccggat
     4201 cctcgacgac accgagctct cgatcgccga ggtcagccgc gccgcggggt tcacgacggt
     4261 gcggcacatg aaccgcgtca tgaagcaggt gttccagttc acgccgcagg agctgcgcgc
     4321 gaagcggcgc gacccgcgcc gcctcgtcgc cgacggcggg ctcgagctgc gcctgccgta
     4381 tcgcgcgcca ctcgcgtggg acacgatgat ccgctacctc gcgccgcgcg ccatccccgg
     4441 tgtggaagcg gtcgacgtcg acgccggcgt ctaccggcgg acgatcgtct tcggcgacgc
     4501 acccggtgtg atcgaagtcc gggacagtga cgaacgcacg cacgtgttgt tgcgcgtgca
     4561 cctgtcgcgc ttcgacgggc tggtgcacct cgtccagcag gtgcgccgcc agttcgacct
     4621 cgatgccgat ccggaagcga tcgacgcgtt cctcgcccgc gacccgaagc tgcggccact
     4681 cgtgcgtcgc caccgcgggc tgcgcgtccc cggtgcgtac gacccggtcg agctcggtgc
     4741 gcgggcgatc ctcggccagc aggtcacggt ggcgggcgcg acgcggctcg cggggcgcgt
     4801 cgtcgaggcg cacggcaccc cggtgccggg tctcgaggcg ctcgggctca cccacacgtt
     4861 cccgagcgcc gcggtgctcg gcgacgcgcc actcgagcgg ctcggcctca cggccgcgcg
     4921 ggcgcgggcg gtccgggagt tcgcggccgc cagtcccaag ctccgattcg acggcgggat
     4981 gcccctcgcc gagctggtcg gcgtcctggt cgatttgccg ggaatcgggg cctggaccgc
     5041 gcactacatc gcgatgcgcg ccgccggcga gcgcgatgcc ttccccgccg gtgacctcgg
     5101 tctgcgcaaa gcgctcgacg caccgtccgc gcacgcggtg ggcgaggcgt cgaatcggtg
     5161 gcggccgtgg cgcgcgtacg ccgccatgca gctctggtgc tcgacgtccg cctgacctga
     5221 acacgtcgtt ttttctccgc gttttcccag gtcgtggccg tttttcggct tgagcagcgg
     5281 ggcaaactcg ggtaaggtcg aacaccggct tttgccggga cgtaccgctc cgctgccccc
     5341 cgtctgctgc ggagattgcg gggaacaacc cgcgccggta cggcgtttca cagttgttac
     5401 accggagtcg gcacccggcc gatactccct ttaagccccg cgaaggaagc catgcccgcc
     5461 aaggtcaaca agtcccgcag cgtcgaccgt gactacgaag acctcgtccg cctgtacctc
     5521 gaggatgttg gccgccacga cctgctgacc aaggacgacg aggcccgcct cgcccaggcc
     5581 atcgaggccg gcgtcgctgc ccgcgccgaa ctggacgcgg gcaagcgcct cacgccgacc
     5641 cagcgccgca acctccgccg cgacgtccgc cggggcgagg aggcgcaccg ccagttcgtg
     5701 aactcgaacc tgcggctggt cgtctcgatc gcgaagaagt accagtcctc gggcctgccc
     5761 ctgctcgaca tcgtgcagga gggcaacctc ggcctgatcc acgccgtcga caagttcgat
     5821 tggcgcaagg gcttcaagtt ctcgacgtac gcgacgtggt ggatccgcca ggcgatccag
     5881 cgcggcatcg cgaactcggc gcgtgtcatc cgtctgccag tgcacgccgg cgacatgctc
     5941 accgcgctgc tcaagctgcg ggcgcagctc gagggttcgc tgggccgcac gccgacgctg
     6001 gccgagctcg cgaccgaggc cgagcttccg ctcgacaagg tcgtcgaagt gctccgctac
     6061 gcggtcgaca ccgtctcgct cgacgagccg gtgcgcgacg acggtgacgc cgagctgggt
     6121 gacttcgtcg aggaccgcaa cgcaaccgcg ccgttcgacc acgcggcaac gtcgctgctt
     6181 ccgggtgaag tggcgaaggt gctggccgtt ctcgacgacc gcgagcgcac catcttgacg
     6241 ttgcgcttcg gtctcgacgg tgggggcgag tgcacgctcg aggaagtggc cgagcacttc
     6301 ggtctcaccc gtgagcggat ccgtcagatc gaagctcgcg cgatgtcgaa gctgcggcac
     6361 ccgtccgccg acgtcggcgc ccgcgagctc ctcgaagccg tctgagcttc acgcatcctt
     6421 gcaatgaacg gccgccctcc ggggcggccg tctcgcgtct ccgcggcgta cgctggaggc
     6481 caactcatga caggggcatt tcggatcgcg cgcaatcctg atcccgactc gacattgccg
     6541 tatgtgttgt ccgtgccgat cggcgcaacg ccactggtgc tgaaggccaa ggacacatgg
     6601 ccccgcactg cgaaggtgta ctgccaccgc ggcgcctggc ccgaggacgc ggagatcgtg
     6661 gaggagacgc cggtccggtc gtgcgtccgg cgtggagtcg cgatcgacct cgtactcgac
     6721 cgcgcgcgag aaagccgatc gcagttcgtc atcacgacgc tgaagggcgg acgggagggc
     6781 atcttctggc agtcggcccg gaccaaccgc aagacgcgac ccgggatgcg tctcccgacg
     6841 aagcggagtg ccgcgggtgt cctcgaggtc gtcgtcgaca ctcgtgagcg ttacgcctgg
     6901 aagttcggcg atcagcaagt cgtgatcgag cggcgagcgc tgcgcgtcgg cgactatgcg
     6961 gtcgagttcg acggagaagt cgtcggcgtc gtcgaacgga agaagcttgc ggagctc
//
LOCUS       DASUGA010000710         2381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1322897, whole genome shotgun sequence.
ACCESSION   DASUGA010000710 DASUGA010000000
VERSION     DASUGA010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322897"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /locus_tag="VFX21_14095"
     CDS             <1..1240
                     /locus_tag="VFX21_14095"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX5097153.1"
                     /translation="PFVARNATRFVDLAGRLDISFDDFIRTSADPRHRPVVEEIWRRS
                     LDNGDLYRATYAGWYCAGCEAFVDGACDEHDTPLEHVEEENWYFRLSRYAPVIRERIE
                     SGALRIAPAERANEVLGFLAGEVRDISVSRSRARARDWGIPVPGDDGQIVYVWFDALV
                     NYLSAAGFERWDAARVRRHVIGKGIVRFHAVIWPAILLAAGVRLPDELFVHDYVTAAG
                     RKIGKSLGNVVDPGAIVDRYGADTLRWWLLREVPRVGETDFSEERLVDTANRDLANGI
                     GNLVQRVVTLAARDGIGGGVPSDDAWPLIAVCHETRTAIDAAFETFDLRAATGALCDL
                     VGAVNRYVEHEKPWTLPPDARGPVLATALHATRVVADELTPFAPTLAGAAAARLGRAE
                     GPVVPGPPLFGRLEAAKSTA"
     gene            complement(1241..>2381)
                     /locus_tag="VFX21_14100"
     CDS             complement(1241..>2381)
                     /locus_tag="VFX21_14100"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX5097154.1"
                     /translation="GTAGSASAATANPVCDAFSADANGNPTGAALATGVALPALNLPD
                     LTGGPVETGTAFEASTPSSPVTLPADTTIEFPPGSGTMISATVAEVKNMKLTFDTAGA
                     ASIGQPVLSGGNVLGASAVVNNLQMVFTLPGSQSGSTVGGGDAYFPGGSTFTPPALAI
                     PVAAPNSPGTITTTLTGLTLNVKVLIGGVTPLPIALLCTANAQIGSVSVVAPPPPPPG
                     APDAVNDTATTEKGTAVTVDVTKNDTKDSTQAALDIDYSSLAITSNPTKGKAEIVDGK
                     AVYTPNADAVGTDSFKYTLCSVLPATGGEAACDIATVSITITDAAAASTTTTTVQAAA
                     APTTVAAAELPRTGSSSTPLALIGVFVVASGLSLAGLFRRPRHAR"
BASE COUNT          341 a          803 c          850 g          387 t
ORIGIN      
        1 gccgttcgtc gcgcgcaacg ccacccgctt cgtcgatctc gccggccgtc tcgacatctc
       61 gttcgacgac ttcatacgaa cgagcgccga tcctcgtcac cggcccgtcg tcgaggagat
      121 ctggcgtcgc agcctcgaca acggcgacct ctaccgagcg acgtacgccg gttggtactg
      181 cgcgggatgc gaggcgttcg tcgacggcgc ctgcgacgaa cacgatacgc cgctcgagca
      241 cgtcgaggag gagaactggt acttccgcct ctcgcggtac gcgcccgtga tccgggagcg
      301 catcgagtcg ggtgccctgc gcatcgcacc cgccgaacgg gccaacgaag tgctcgggtt
      361 cctcgcgggt gaggtacgcg acatcagcgt gtcgcgatcg cgcgctcgcg cgcgcgactg
      421 gggcatcccg gtcccgggtg acgacggcca gatcgtctac gtgtggttcg acgcgctggt
      481 gaactacctc agcgcggccg gcttcgaacg gtgggacgcg gcacgcgtcc gccgccacgt
      541 gatcggcaag ggcatcgtgc gcttccacgc cgtcatctgg cccgcgatcc tgctggcggc
      601 gggtgtccgg ttacccgacg agctgttcgt gcacgactat gtcaccgccg cgggccgcaa
      661 gatcgggaag tcgctgggca acgtcgtcga tcccggtgcg atcgtcgacc gttacggcgc
      721 cgacacgctg cggtggtggc tcctgcgcga ggtgccgcgc gtcggcgaga ccgacttcag
      781 cgaggagcgt ctcgtcgaca ccgcgaaccg tgatctcgcg aacggcatcg ggaacctcgt
      841 gcagcgcgtc gtcacgctcg cggcgcgcga cggcatcggc ggcggtgtac cgagcgacga
      901 cgcgtggccg ctcatcgccg tgtgccacga gacgcgcacc gcgatcgacg ccgcgttcga
      961 gacgttcgat ctgcgcgccg cgaccggcgc gttgtgcgac ttggtcggcg cggtcaaccg
     1021 ctacgtcgaa cacgagaagc catggacact ccctcccgat gcgcgcggcc ccgtgctcgc
     1081 gacggcgctg cacgcgacgc gcgtcgtcgc cgacgagctc acgccgttcg caccgacgct
     1141 cgccggggcc gcggcggcgc ggctggggcg cgcagagggc ccggtcgtcc cgggccctcc
     1201 gttgtttggc cgtctcgagg ccgctaaatc gacagcgtga tcagcgagcg tgtcgtggac
     1261 gccggaacaa gccggccaat gaaagaccac ttgcgacgac gaacacgccg atcagagcca
     1321 acggtgtcga cgagctgccc gtacgtggca gctcggcggc ggcgacggtc gtcggcgcgg
     1381 ccgcagcctg caccgttgtc gtcgtcgtgg atgccgcggc ggcatcggtg atcgtgatcg
     1441 acacggtcgc gatgtcgcag gccgcttccc ctccggtggc cggcagcacc gagcacagtg
     1501 tgtacttgaa ggagtcggta ccgaccgcgt cggcgttggg cgtgtacacg gccttgccgt
     1561 cgacgatctc tgccttgccc ttggtcgggt tgctcgtgat cgcgagcgac gagtaatcga
     1621 tgtcgagggc ggcctgggtg ctgtccttgg tgtcgttctt cgtcacgtcg accgtgacgg
     1681 ccgtgccctt ctcggtcgtc gcggtgtcgt tgacggcgtc gggcgcgccg ggcggcggag
     1741 gaggcggcgc gacgaccgaa accgaaccga tctgcgcgtt cgcggtgcag aggagcgcga
     1801 tgggaagcgg tgtcacgccg ccgatgagaa ccttgacgtt cagcgtcaga ccggtgagtg
     1861 tcgtggtgat cgtgcccggg ctgttcggcg cggccaccgg gatcgcgagt gccggcggcg
     1921 taaaggttga accaccgggg aagtacgcat caccgccgcc gacagtgctg ccgctctgcg
     1981 agccgggcag cgtgaagacc atttgcaggt tgttcacgac cgcggatgct ccgagcacgt
     2041 tgccgccgct caagaccggc tgcccgatcg acgctgcgcc ggcggtgtcg aacgtcaact
     2101 tcatgttctt gacctccgcg accgtcgcgc tgatcatcgt gcccgagccg ggtgggaact
     2161 cgatcgttgt gtcggccggc agggtgaccg gcgacgacgg cgtcgacgct tcgaaggccg
     2221 tcccggtttc gactggccca cccgtcaggt cggggaggtt caacgccggc aacgcgacac
     2281 cggtggcgag cgcagcgccc gtcgggttgc cgttcgcgtc ggccgagaac gcgtcgcata
     2341 ctggattcgc ggtcgccgcg gacgcggacc ccgcggtgcc g
//
LOCUS       DASUGA010000711         3008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1669348, whole genome shotgun sequence.
ACCESSION   DASUGA010000711 DASUGA010000000
VERSION     DASUGA010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669348"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VFX21_14105"
     CDS             <1..920
                     /locus_tag="VFX21_14105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5097155.1"
                     /translation="RLIWLAIGALIALLIALTIADLTIGRRVRSLVGVTRQIGAGNLS
                     ARSDVGTGDEVGDLGKALDAMAAELRARDEERAQLLTKLVDASEEERRRIAGDVHDDS
                     IQVMSAQVMGLQLLRRRVDDPELKARIQELEESGREATARLRDLVFYLHSPTLETHGL
                     ASAIDLLLDRSFEGETVEWSVKSALAEDPPPASRDTAYRIAQEAIGNARRHARAAHIT
                     VDLRRDGNELVMTIVDDGVGFSADGQADRPGHLGLRAARERAAAVGGAVVIDSAVGGG
                     TRFSCRLPWNFDAVATPPNGERAAVRTDP"
     gene            complement(904..1269)
                     /locus_tag="VFX21_14110"
     CDS             complement(904..1269)
                     /locus_tag="VFX21_14110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5097156.1"
                     /translation="MTDVRVLLADDEEIVRDAIGALLEEDPRFRVVAAVATASAAAAA
                     ATTLQPDLAVIDVRMPGGGRAAIAAIREHSPETVVLVCSSMDDRYTRSTMAEAGAAAY
                     VVKGDDDVLDAARGVMGLS"
     gene            1369..1668
                     /locus_tag="VFX21_14115"
     CDS             1369..1668
                     /locus_tag="VFX21_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEX5097157.1"
                     /translation="MIVYAVVSDLMDRSRLAAAVPGLRFARTAGECADGDVIVVDLTR
                     GTELVAALRGAAPGARIVAYGPHVDADGLAAARAAGADTAVPRSRFFRDIPAAIA"
     gene            1700..>3008
                     /locus_tag="VFX21_14120"
     CDS             1700..>3008
                     /locus_tag="VFX21_14120"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5097158.1"
                     /translation="MPHSLLSRLEAAAQSRGGITFLGSAGGETGEHRISYAQLHDDAR
                     AMAARLQARGIGPRTNVALLGPTTRPLVTAIQATWCCGAATVMLPLPMRLASIEEFVA
                     QTRARVRASDTALVIVDSQLAEFLDPQPGDPPMVLLDELHAAGGRTDAFERPADDPDA
                     LAILQYTSGSTADPRGVMLPHRCVAANIDAIMQAATLDPGVDVGVSWLPLYHDMGLIG
                     LLTLPMTTGMELALAAPQDFLAAPAQWMQWMSRFHGSATAGPNFSYALAARALRRLDD
                     LDLSRWTLALNGAEPVDPGAVEAFVAAGAPHGLAATAAFPAFGMAEATLAVTFPEKGS
                     GLAVDTIDGRVLETDRYAATVETSAPGARRLAKLGRPVPGLELRICDSSTGTEMHERE
                     VGELEIRGASVTPGYYGRPDITEATFHDGWLRSGDLGYLVDGEL"
BASE COUNT          432 a         1086 c         1041 g          449 t
ORIGIN      
        1 tccggctgat ctggctcgcg atcggcgcgt tgatcgcgct gctcatcgcg ctgacgatcg
       61 ccgacctgac gataggccgt cgcgtccgtt cactggtggg cgtgacccgc cagatcggtg
      121 ccggcaatct gagtgcgcgt tcggacgtcg ggaccggcga cgaagtcggc gacttgggca
      181 aggcgctcga cgcgatggct gccgagctgc gcgcccgaga cgaagagcgc gcgcaacttc
      241 tcacgaagct ggtcgacgcg agcgaggagg aacggcgccg tatcgcgggc gacgtacacg
      301 acgactcgat tcaggtgatg tcggcccaag tgatgggact ccagctgttg cggcgccggg
      361 tcgacgaccc cgagctgaag gcgcgcattc aggagctcga ggagtcgggc cgcgaggcaa
      421 ccgcccgact gcgcgacctc gtcttctacc tgcactcgcc gacgctcgag acccacgggc
      481 tcgcgtccgc gatcgacttg ttgctcgacc gctcgttcga aggcgagacg gtggagtggt
      541 cggtcaaaag tgcgctcgcc gaggacccgc cgcccgcgag tcgcgacact gcgtatcgca
      601 tcgcgcagga agcgatcggc aacgcgcgcc gccatgcacg tgccgcgcac atcaccgtcg
      661 acctccggcg cgacggcaac gagctcgtga tgacgatcgt cgacgacggc gtggggttct
      721 ccgccgacgg ccaggctgat cggcccggtc acctcggact gcgcgccgcg cgcgagcggg
      781 ctgcggccgt cggtggcgcg gtcgtgatcg actcggccgt cggaggcggc acgcgcttct
      841 cgtgccgtct cccgtggaac ttcgacgccg tcgcgacgcc gccgaacggc gaacgcgccg
      901 cggtcaggac agacccatga cgccgcgcgc ggcgtcgagc acgtcgtcgt cacccttgac
      961 gacgtacgcc gcggcaccgg cctcggccat ggtgctgcgc gtgtagcggt cgtccatgct
     1021 cgagcacacg agcacgacag tctcgggcga gtgctcacgg atcgccgcga tcgcggcgcg
     1081 tccaccgccc ggcatacgca cgtcgatcac cgccagatcg ggctgcaacg tcgtcgccgc
     1141 ggccgcggcc gccgacgccg tcgcgacggc cgcgacgacg cgaaagcgcg ggtcttcctc
     1201 cagcaacgcg ccgatcgcgt cccggacaat ctcttcgtcg tcagcgagca gcacccgcac
     1261 gtcggtcatg gttcccagcc gttgcctcgg tcatcagcat gcgctgcgca gccgcgtggc
     1321 gcatgacacg ggcaggtcat tcggagtgat cagacgtagc ctgatcggat gatcgtgtac
     1381 gcggtcgttt cggatctgat ggatcgctcc cggctcgccg cggccgtgcc cggcctgcgc
     1441 tttgcccgca ccgcggggga atgcgccgat ggcgacgtca tcgtcgtcga tctcacgcgc
     1501 ggcaccgagc tcgtcgctgc gctgcgcggg gccgcgcctg gagcccgcat cgtcgcgtac
     1561 ggcccgcacg tggacgcgga tggcctggcc gcagcccgcg ccgccggtgc agacaccgcg
     1621 gtgccccgat cgcgcttttt tcgcgacatt cccgccgcga tcgcgtaacc ctcacgggtg
     1681 atcgtgggtc ggaagcctcg tgccccactc tctgctctcg cgcctggagg ccgccgcgca
     1741 gtcccgcggt ggcatcacgt tcctgggatc ggcgggaggt gagaccggcg agcaccgcat
     1801 ctcgtacgcg caactgcacg acgacgcgcg cgcgatggcg gcgcggttgc aagcacgcgg
     1861 tatcgggccg cgcacgaacg tcgcgttgct cggaccgacc acgcgtccgc tcgtcaccgc
     1921 cattcaggcc acgtggtgct gcggtgcggc aacggtcatg ctgccgttgc cgatgcggct
     1981 ggcgtcgatc gaagagttcg tcgcccagac gcgcgcccgg gtgcgcgcgt ccgacaccgc
     2041 gctcgtgatc gtcgactcgc aactcgcgga gttcctcgac ccgcagccgg gcgatccgcc
     2101 gatggtgttg ctcgacgaac tgcacgcggc cggcggccgc acggatgcat tcgaacgtcc
     2161 cgccgacgac ccggatgcgt tggcgatcct gcagtacacg agcggctcga cagccgatcc
     2221 gcgcggcgtc atgctgccgc accgctgcgt cgccgccaac atcgacgcga tcatgcaagc
     2281 ggccacgctc gacccgggcg tcgacgtcgg cgtgtcgtgg ctgccgctgt atcacgacat
     2341 gggactgatc ggtctgctca cactgccgat gaccaccggc atggaacttg cgctcgcggc
     2401 accgcaagac ttcctcgccg cgcccgcgca gtggatgcag tggatgtcgc gcttccacgg
     2461 cagcgcgacc gccggcccga acttctccta cgcgttggcg gcgcgcgcgt tgcgccgcct
     2521 cgacgatctc gacctgtcgc gctggacgct cgcgctgaac ggcgccgagc ccgtcgaccc
     2581 cggcgccgtc gaagcgttcg tcgcggccgg cgcaccgcac ggactcgcgg cgaccgccgc
     2641 gttccccgcg ttcgggatgg ccgaggcgac gctcgccgtc acgttccccg aaaagggatc
     2701 gggcctggcc gtcgacacga tcgacggccg cgttctcgaa accgaccgct acgccgcgac
     2761 cgtcgagacg agcgcgcccg gcgcccggcg actcgccaag ctcgggcggc ccgtgccggg
     2821 actcgagctg cggatctgcg actcgtcgac ggggaccgag atgcacgaac gcgaagtcgg
     2881 cgagctggaa attcgcggcg cgtcggtcac gcccggttac tacgggcggc ccgacatcac
     2941 cgaggcgacg ttccacgacg gctggttgcg cagcggtgac ctcggctacc tggtcgacgg
     3001 tgagctcg
//
LOCUS       DASUGA010000712         5768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_315386, whole genome shotgun sequence.
ACCESSION   DASUGA010000712 DASUGA010000000
VERSION     DASUGA010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315386"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VFX21_14125"
     CDS             <1..473
                     /locus_tag="VFX21_14125"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5097159.1"
                     /translation="AEWIDDLMRAARERLASLGDQLLTMTHGDWRIDNVRVVANEVVA
                     VYDWESVGPIPETEAVAAAARTFSVDWDRDDVARFPAPAAMAAFIGEYERARGAPLTA
                     TERAHTAFTMVASMAYGARCEHSIQWPEIEDSQQRQLRALGPALLRVGLDALHA"
     gene            1687..4119
                     /locus_tag="VFX21_14130"
     CDS             1687..4119
                     /locus_tag="VFX21_14130"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX5097160.1"
                     /translation="MDVETRPRLREILTRRDVAIIGVDVDIERPQRVRAASDVLRLLV
                     GLGFVVVGVLLATVADRTVGGAQADLIEAVSRIPSRVEQAIIGLAQLLTGSVITIVAV
                     VMIVQRHWRRLGVLILGALTAAGVMMLLTNVLDLPQTQAAIDAYGDPIGWLTSPGFPS
                     ARYLAATAAVVTLASVWLSRPWKRALWAAVVVLMTLRALSSSTSSLDVVLAVAVGVVV
                     GSLALVVFGAPNRAPDAYDLVTALRELLPDLRSLHQRHTGGGALTFVAATEDHEYFVK
                     LRTPDDRSADYLARLYRSIRLRAYEVEPPYTTVQRRVEHEALVILRADAAGVSTAALT
                     GILATEDDSAMLVTTYVGGERLADVERERVGPALLEELWTQVSALDRARIAHRNLALE
                     NVILDRAGGLRLVDFDDAELAASERRRNHDVAQVLVETSILVGIDDAVEAALTQLGPE
                     RLAAAVPLLQPLALSPAVRTRLRAATISLDELRATVLRRTNAPEVPLERLERVRPRTL
                     LSVIALTAAFYFVLPQLANLGDTFRAFGDAEWPWLVGALVASATTYFFAALAFVGSVS
                     EPVPFGPAWRSRLATSFTSIITPAGTGGMALGIRVLQRMGIDATAATASVGINQIAGV
                     LVHVTLLFGFIAWTGRAGLGGFSLPDSTVLLLGIAIILGLSGLALTIRRIRNLVLQPV
                     LRTARAAGSELAGVVRNPVRVLSLLGGSAGTTLSYVVALAASIQAFGGGLSYPQIGAA
                     YLGASLVGNAAPTPGGLGAVEAALVAALTGFGLDNGHAVSAVLTFRLATYWIPTLPGW
                     FVFRWMQTHDEI"
     gene            4156..>5768
                     /locus_tag="VFX21_14135"
     CDS             4156..>5768
                     /locus_tag="VFX21_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097161.1"
                     /translation="MTALSDRTEDAAAPTVDIVEPNWPTTERSPVQLLSLVAAAAGFV
                     CALSVAQLDRDALFGLESDTLRYFDELPDALERFLLGVSQFTAIVYPVILAVAFLALR
                     RWRPVLIAVLGAAIGAGLAFGVNELLDRPQPPELAPARAVDTWIGSARFPSYLYIAAA
                     AAAAIVVGAYAGRRWRFVAWGIVGFVALFRVLSGTELPVTLALAATVGWAAGAGVLLV
                     FGVPTHRATGRQVADALARSGLAVRRLAPASVDARGSTPWFATTQEGERIFVKALGRD
                     ERDADLLFRIYRFFRLRNVGDQRPFSTLRRSVEHEALVALKARDVGIRTPHLLTLATC
                     EPNGFLLAYAMVDGASLDRVDQPWSDDLLRGIWEQVALLRHQRIAHRDLRRANVFVDD
                     DGAPWIIDFGFSELAVTDDMLAQDLAQLLAALAVGAGAQRSVSSAIAVLGPEAVGTAL
                     PYLQAAAFANATRKALAANKGLLDELRDTVQQEAHVEAVHYEPLQRFSIRPAFMLVAS
                     LIALYVLIPQLADVEGMFKQLKDANWLLV"
BASE COUNT          808 a         2058 c         1997 g          905 t
ORIGIN      
        1 gtgccgaatg gatcgacgac ctgatgcgcg cggctcgcga gcggctcgcg agcttgggcg
       61 atcagctcct gacgatgacg cacggcgact ggcgcatcga caacgtccgc gtcgtcgcca
      121 acgaagtcgt cgcggtctac gactgggaga gcgtcgggcc gatccccgag accgaggcgg
      181 tcgcggcggc ggcccgcacg ttctcggtcg actgggaccg cgacgacgtg gcgcgcttcc
      241 ctgcaccggc ggccatggcc gcgttcatcg gcgagtacga acgtgcccgc ggcgcaccgt
      301 tgaccgctac cgagcgcgcc cacaccgcgt tcacgatggt ggcgtcgatg gcgtacggcg
      361 cccgctgtga gcactcgatc caatggccgg agatcgagga ctcacaacag cgccagctcc
      421 gcgcgctcgg tcccgcgctg ttgcgcgtcg gcctcgacgc gctgcacgca tgacgatgtc
      481 gacgagcagc ccggcgccga tcgcgcaagc caggtcgaag ggtgcacgca agcgtggcgg
      541 cgacaggatc aggagcgaca gcagcgcgaa ctgcaggacg atcaccgcca accactgcac
      601 ccgcacgtcg cggcggtagt gccacaaccc gaccacaccg gccaccgtga cgaagtagaa
      661 gagcggcaac gtcgcgtggc ggaagtggag gttccgcccg tcgatgcgtt cggggctgtc
      721 gttctcgctc ggtgtcagtt cgaagaactc gagcaccgtg cgcgccgagt tctccacgac
      781 gtagccggga ttgtcccgga gcccgtcgag cgcgcggtcg gtcagctcgt cgttccagtc
      841 ggcctcgtcg aactgcgaca gccgcagctc gagatcgtcg tcgtacgcgt gcgagaagac
      901 cgggtcgacg aagccgtcgg cgtcttgggc cggaggcgcg aagaccgcgg ccagcgtgaa
      961 ggcgtcggac gtgacgaggc ggggtgtgcc gacctggacg atgttgcgca ctgaccacgg
     1021 cacgacgacc gcgagcgcga tcgcggtcgc gacgactgct gtcttcgtgc ccgcgcggcg
     1081 gaacgcgacg aacacgacga gcgcgacgac cagataggcg ttcggacgcg tcaacacgag
     1141 cgcgcccgcg agcgcggcgg cccacagcca gtgcgcgtcg tccatcagca acgcgatgcc
     1201 gagcacgagg agcaacgcga gcggctcggt gagcgtgatc gtgtcgttcg cgagcagcgg
     1261 cggatacaac gcgacgacca gaccggcgca caacccggcg cgcggtccgc cgatccgttg
     1321 cgcaagcacc gccgcgagga gcacgacgcc ggtgccgagc gcgatgctca acaggcgggc
     1381 cggccacagc gcgtccgtcc atccgaccgc cgccaacaac gcgggataca gcggtggccg
     1441 gaacgcggtg tcgtgcactg cgatctgcgg gtactgcaac gagaacccgt cgccgtgggc
     1501 cagcgcccgt gcgatcgaga cgtactggcc cgcgtccgag tcgggtgtcc acgacggcgt
     1561 caccagcacc caatacgtga ggcgggcgac gacggcgaca ccggcgacga tccacaggtt
     1621 gcgaccggca cgtacgcgag cgatggcgca ccctagatgc ccgcgcgtcc cgtagcgttc
     1681 cggcccgtgg acgtcgagac caggcctcgg ctgcgcgaga tcctgacgcg ccgggacgtc
     1741 gcgatcatcg gcgtcgacgt cgacatcgaa cgaccgcaac gggtgcgcgc ggcgagcgac
     1801 gtgctgcgct tgcttgtggg gctcggcttc gtggtcgtcg gcgtcctcct cgcgaccgtc
     1861 gcggaccgga ccgtgggtgg cgcgcaggcc gatctcatcg aggcggtgtc gcgcatcccg
     1921 tcgcgggtcg agcaggcgat catcgggctc gcgcagttgc tcacgggcag cgtgatcacg
     1981 atcgtcgcgg tcgtcatgat cgtgcagcgc cactggcgcc ggctcggtgt gctcatcctc
     2041 ggcgcgctca cggcggccgg cgtcatgatg ttgctcacca acgtgctcga tctgccgcag
     2101 acgcaggccg ccatcgacgc gtacggagac ccgatcggat ggctcacgag ccccggcttc
     2161 ccgagcgcgc gctacctcgc tgcgaccgcc gcggtggtga cgctcgccag cgtgtggctc
     2221 agccggccat ggaagcgcgc gctgtgggcc gcggtcgtcg tgttgatgac cctgcgggcg
     2281 ctgtcgtcgt cgacgagctc gctcgacgtc gtgctcgcgg tcgccgtcgg cgtcgtcgtc
     2341 gggtcgttgg cactcgtcgt cttcggcgca cccaaccgcg cgccggacgc gtacgacctc
     2401 gtcaccgcgc tgcgcgagct gcttcccgac ctccgatcac ttcaccaacg ccataccggt
     2461 ggcggcgcac tgacgttcgt cgccgccacc gaagatcacg agtacttcgt gaagctgcgc
     2521 acgccggacg atcgcagcgc cgactatctc gctcgcctct accgcagcat ccgcctccgg
     2581 gcctacgagg tcgagccgcc gtacacgacc gtgcaacgcc gggtcgagca cgaagcactg
     2641 gtcatcctgc gggcggacgc ggccggagtg agcaccgcgg cgctgaccgg catcctcgcg
     2701 accgaggacg actcggccat gctcgtgacg acgtacgtcg gaggcgagcg attggccgac
     2761 gtcgagcgcg agcgcgtcgg ccccgcgctg ctcgaggagc tgtggacaca ggtctccgcg
     2821 ctcgaccggg cccggatcgc gcaccgcaac ctcgcgctcg agaacgtcat cctcgatcgc
     2881 gcgggcgggc tccgcctcgt cgacttcgac gacgcggagc tggccgcatc ggagcgacgg
     2941 cgcaaccacg atgtcgcgca agtgctcgtc gagacgtcca tcctcgtggg catcgacgac
     3001 gccgtcgagg ccgcgctcac gcaactcggt ccggaacggc tggccgccgc cgtaccgctg
     3061 ttgcaaccgc tcgcgctctc gcccgccgtg cgcactcggc tgcgcgccgc gaccatctcg
     3121 ctcgacgagc tgcgcgccac cgtcttgcgt cgcaccaatg cgcccgaagt gccactcgaa
     3181 cgcctcgagc gagtacggcc ccgcacgctg ctctcggtga tcgcgctcac ggccgcgttc
     3241 tacttcgtcc tcccgcagct cgcgaacctc ggcgacacgt tccgcgcgtt cggcgacgcc
     3301 gagtggccgt ggctggtcgg cgcgctcgtc gccagcgcga cgacgtactt cttcgcggcg
     3361 ctcgcgttcg tcggatcggt gtcggagccg gtgccgttcg ggccggcgtg gcgctcgcgg
     3421 ttggcgacgt cgttcacgtc gatcatcacg ccggcgggca cgggtggcat ggcgcttggt
     3481 atccgggtgc tgcaacgcat gggcatcgac gcgaccgcgg cgacggcgtc ggtcggcatc
     3541 aaccagatcg cgggcgtgct cgtgcacgtc accttgctgt tcgggttcat cgcgtggacc
     3601 ggtcgtgccg gcctcggcgg cttctcgctt cccgacagca ccgtgttgct gctcggcatc
     3661 gccatcatcc tcgggctctc cggtctcgcg ctcacgatcc gccggatccg caacctcgtc
     3721 ctgcagccgg tgctccggac ggcgcgagcc gcgggcagtg agctcgcggg cgtcgtccgc
     3781 aacccggtgc gtgtcctgtc tctgctcggc ggctccgcgg ggacgacgct ctcgtacgtc
     3841 gtcgcgctcg cggcgtcgat ccaggcgttc ggcggcggcc tcagctaccc gcagatcggt
     3901 gccgcgtatc tcggcgcgtc cctggtcggc aacgcggcgc cgacaccggg cgggctcggc
     3961 gcggtcgaag ccgccctggt cgctgcgctg accgggttcg ggctcgacaa cggccacgcc
     4021 gtgtccgctg tcctcacgtt ccggttggcg acatactgga ttcccaccct gccggggtgg
     4081 ttcgtcttcc ggtggatgca gacgcatgac gagatctaat gtcgagatct caccccaacg
     4141 gataagcatt ccggcatgac ggcgctgtcc gaccggaccg aagacgcggc cgcaccgacc
     4201 gtcgacatcg tcgagccgaa ctggcccacg accgaacggt cgcccgtgca gctgctgtcg
     4261 ctcgtcgcgg ccgccgccgg attcgtgtgt gcgctgtcgg tcgcccagct cgaccgcgac
     4321 gccctgttcg gcctcgagtc cgacaccctg cgctacttcg acgagcttcc ggacgcgctc
     4381 gaacggttcc tgctcggcgt gtcgcagttc acggcgatcg tgtacccggt gatcctggcg
     4441 gtcgcgttcc tcgcgttgcg gcgctggcga ccggtgctga tcgcggtgct cggcgcggcg
     4501 atcggcgccg gcctcgcgtt cggtgtcaac gaactgctcg accgcccgca gccacccgag
     4561 ctcgcgcccg cccgcgccgt cgacacgtgg atcgggagcg cgcgcttccc gagttacctc
     4621 tatatcgcgg ctgcggcggc ggccgcgatc gtcgtcggtg cgtacgccgg ccggcgctgg
     4681 cgtttcgtcg cgtgggggat cgtcgggttc gtcgcgctgt tccgcgtgct ctccggcacc
     4741 gagctgccgg tcacgctcgc gctcgcggca accgtcggct gggccgcggg cgcgggtgtg
     4801 ctgctcgtct tcggtgtccc gacgcaccgg gcgaccgggc ggcaggttgc cgacgccctt
     4861 gcccgcagtg gcctggccgt gcgccggctc gcgcccgcat ccgtcgacgc ccggggctcg
     4921 acgccgtggt tcgcgacgac gcaggagggc gagcgcatct tcgtcaaggc gctcggccgc
     4981 gacgaacgcg atgccgatct gctgtttcgg atctatcggt tcttccggct gcgcaacgtc
     5041 ggcgaccaac gaccgttctc gacgttgcga cgttcggtcg aacacgaggc gctcgtcgcg
     5101 ctgaaggcgc gcgacgtcgg catccgtacc ccacatctgc tgaccctcgc gacctgcgag
     5161 ccgaacggct tcttgctcgc gtacgcgatg gtcgacggcg cgtcactcga ccgagtggac
     5221 caaccgtgga gcgacgatct gctgcgcggc atctgggagc aggtcgcgtt gttgcgccac
     5281 caacgcatcg cgcaccgcga cctgcggcgg gcgaacgtct tcgtcgacga cgacggcgca
     5341 ccgtggatca tcgacttcgg gttcagcgag ctcgcggtga ccgacgacat gctcgcgcag
     5401 gatctcgcgc agctcctcgc cgcgttggcg gtcggcgccg gcgcgcagcg ttcggtctcg
     5461 tcggcgatcg cggtgctcgg acccgaggca gtcgggaccg cgctccccta tctgcaggcc
     5521 gccgcgttcg ccaacgcgac ccgcaaggca ctcgcggcaa acaaaggctt gctcgacgag
     5581 ctgcgcgaca cggtgcaaca ggaggcgcac gtcgaggcgg tgcactacga acctctgcag
     5641 cggttctcga tccgccctgc gttcatgctc gtcgccagct tgatcgcgtt gtacgtgctc
     5701 atcccgcagc tcgcggacgt cgaggggatg ttcaagcagc tgaaggacgc gaactggttg
     5761 ctcgtctt
//
LOCUS       DASUGA010000713         3378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_907232, whole genome shotgun sequence.
ACCESSION   DASUGA010000713 DASUGA010000000
VERSION     DASUGA010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907232"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /locus_tag="VFX21_14140"
     CDS             complement(<1..1406)
                     /locus_tag="VFX21_14140"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEX5097162.1"
                     /translation="MTRRLGRCVLGAIVACGLLSLGGLSEAAPAAAASPTVTGPVTGG
                     GGVPLVYSGQPADALNTNPAFDLASVGYVQQEFFVEGTASAYSPTAPLTTDGHWTVAP
                     SSTAAYKTRIVVNRPERSRDFNGTVVVEWLNVSGSVDASPDWQHTHVELIRRGYAWVG
                     VSAQAVGVNYLKCPDPPVPSCPTSIQGQQPGDAARYGSLNHPGDSYSYDIYSQAGQAV
                     RDNAALVLGGTRPRRLISVGESQSAGRLATYIDAAHPLARVYDGFLVHSRGAGGAPLS
                     QAPLATVNTPVPTLIRTDLSPVLAFQTETDVANAISGAGLAARQPDTSRYRLWEVAGT
                     SHYDLYGLKTGSTDVGKQESVDAWFDSMLHPTNEPIPLFVCGTPINSGPQTFVLRSAI
                     AALDRWAAGGPPPPSAPRLEVASVSPLNFALDSLGNVRGGIRTPAVDAPVAALRGQGQ
                     TGTAFCSLFGTTVPLTQA"
     gene            complement(1904..2698)
                     /locus_tag="VFX21_14145"
     CDS             complement(1904..2698)
                     /locus_tag="VFX21_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5097163.1"
                     /translation="MTAYEFIEVDTVADGVRRVTLNRPEKRNAISTPLRTELLDALRA
                     HDSDPDARVTIVRGAGPCFSSGYDLGDDLMKNAPFYSAPGDGQWARQANDTWFSVWDL
                     AKPVIAQIHGYAIAGATELASACDLVYVADDALISYPVVRVVSPPDWQYHTVLLGLRR
                     AMELILTGDAIDGVEAERVGFANRAFPADRLDDEVLKVAIRIAGVPSDLTQINKRSVH
                     RAFDVWGARAAIRAGTELQALAGHTETAKQARANILGAVKKTIGND"
     gene            complement(2695..>3378)
                     /locus_tag="VFX21_14150"
     CDS             complement(2695..>3378)
                     /locus_tag="VFX21_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5097164.1"
                     /translation="LRLRDYVTIMRKVLDREEPVVHDGPELQLPYRGPGALGQGKALK
                     SIMHPVAPIPIWLASGGPKNNALAAELFDGWLPMGLGPDGVPAAMREREKDGFEVFTG
                     AAVNITDDVQGALDAMRPLTAMYVGGMGSETHNYHRDAMARRGFPEAAQRIGELWRAG
                     KKEEAQAAVPDEYLEQNALIGSVERIRRTWHDGYVRDGVTGVIVDARQPEALELMADL
                     AGTRDEVTA"
BASE COUNT          489 a         1165 c         1173 g          551 t
ORIGIN      
        1 tgcgcttgcg tcagcggcac ggttgttccg aagaggctgc agaaggcggt tccggtctgg
       61 ccttggccgc gcagtgccgc gacgggcgcg tccaccgccg gtgtgcgaat cccaccgcgg
      121 acgttgccga ggctgtcgag cgcgaagttg agcggcgaaa cgctcgcgac ttcgagccgc
      181 ggcgccgacg gcggcggtgg tcctccggcg gcccagcggt cgagcgcggc gattgcggac
      241 cgcaacacga acgtctgcgg accactgttg atcggtgtac cgcacacgaa gagcgggatc
      301 ggctcgttcg tcgggtgcag catcgagtcg aaccacgcgt cgacggactc ctgcttgccg
      361 acgtcggtcg agcccgtctt caagccgtac aggtcgtagt gcgacgtgcc cgcgacctcc
      421 cacaggcggt accggctcgt gtcgggttgt cgcgccgcga gaccggcccc ggaaatcgcg
      481 ttcgcgacgt cggtctcggt ctggaacgca agcaccgggg agaggtccgt gcggatcaac
      541 gtcggcaccg gtgtgttgac cgtcgcgagt ggcgcctgcg agagcggcgc gccgcccgcg
      601 ccgcggctgt gcacgaggaa accgtcgtag acccgcgcga gcgggtgcgc ggcgtcgatg
      661 tacgtcgcga gacgtcccgc cgactgcgac tcaccgaccg agatcagacg acgcgggcgc
      721 gtaccgccga gcaccagtgc cgcgttgtcg cggaccgcct gcccggcttg cgagtagatg
      781 tcgtaggaat aggagtcgcc gggatggttc agtgagccat accgcgcggc gtcacccggt
      841 tgctgtccct gaatcgacgt cgggcacgac gggaccggcg ggtcggggca cttgaggtag
      901 ttgacgccga cggcctgcgc ggacacgccg acccacgcgt acccacgccg gatcagttcg
      961 acgtgcgtgt gctgccagtc ggggctggcg tcgacactgc cggacacatt cagccattcg
     1021 acgacgacgg tgccgttgaa gtcgcgggat cgctccggac ggttgacgac gatgcgcgtc
     1081 ttgtacgccg ccgtcgacga cggcgcgacg gtccagtggc cgtcggtcgt gagtggcgcc
     1141 gtcggcgagt acgcgctggc cgtgccctcg acgaagaact cctgttgcac gtaaccgacc
     1201 gaggccaggt cgaacgccgg gttcgtgttg agcgcgtcgg ccggttgacc gctgtagacg
     1261 agcgggacgc cgccgccacc ggtgaccggt ccggtcacgg tcggcgacgc tgccgcggca
     1321 ggcgcggcct cgctcaatcc gccgagcgac agcagtccgc atgccacgat cgcgccaagt
     1381 acgcaacgcc ccagtcgtcg agtcatcccg ggcctcctcg ggttgcgccc ggaacgacac
     1441 ccgcaactac tgctgacgtg gcttgaactg cgtcgccggt ggtgacgcta aatgcccgcc
     1501 gcccaacccg tcaaccagtc cctcgcgcgc gtcggtgcgc tcgacgcgcg tgcggcttgc
     1561 gcatcgcgtc gcgtcgggaa gactgcggca ttgaggaagg agctcaccat tcgtggctcg
     1621 atcgagtacc cggctcgctt cgccgacgcg atcgatctcc tcgcccgcgt cgacatcacg
     1681 cccgtgctca cacatcgcta tcgcctcgag gagttcgacg tcgcgctcga cctgctccga
     1741 ggatccaagg actgcgggaa ggtgctcgtg acgatggaca gcacgcctat catcggggcg
     1801 caacaggcga gcgcgctgag ggctcctcgt tcgcgagggg ccctcacggc caatcgagct
     1861 ccgtatcgct cattctccta cacgtcttcg gccatcggct tggtcagtcg ttgccgatcg
     1921 tcttcttcac cgcgccgagg atgttcgcgc gcgcctgctt ggccgtctcg gtgtggccgg
     1981 ccagtgcctg cagctcggtg ccggcgcgga tcgcggcacg cgcgccccag acgtcgaacg
     2041 cgcgatgcac cgagcgcttg ttgatctgtg tgagatcgct cggcacaccc gcaatgcgga
     2101 tcgcgacctt cagcacctcg tcgtcgagtc gatcggcggg gaacgcgcgg ttcgcgaagc
     2161 cgacgcgttc ggcttcgacg ccgtcgatcg cgtcgccggt gaggatcagc tccatggccc
     2221 gccgcagacc gagcagcacg gtgtggtact gccagtcggg cgggctgacg acgcgcacga
     2281 ccggatagct gatcagcgcg tcgtccgcca cgtagacgag gtcgcatgcc gacgcgagct
     2341 cggtcgcacc cgcgatcgcg tagccgtgga tctgtgcgat gaccggcttg gcgaggtccc
     2401 agacgctgaa ccacgtgtcg ttggcctggc gcgcccactg cccgtcgccg ggcgccgagt
     2461 agaacggcgc gttcttcatc aggtcgtcgc cgaggtcgta acccgacgag aagcacggcc
     2521 ccgcgccgcg aacgatcgtc acgcgcgcgt cgggatcgct gtcgtgcgcg cggagcgcgt
     2581 cgagcagctc ggtgcgcaaa ggtgtcgaga tcgcgttgcg cttctcgggc cggttcaacg
     2641 tgacgcgccg gacaccgtcc gcgacggtgt cgacctcgat gaactcatac gcggtcacgc
     2701 ggtgacctcg tcgcgtgtcc ccgcgaggtc ggccatgagc tcgagcgcct ccggttggcg
     2761 ggcatcgacg atgacgccgg tcacgccgtc gcgcacgtag ccgtcgtgcc acgtgcgccg
     2821 gatccgttcg acggagccga tgagtgcgtt ctgctcgagg tactcgtcgg gcaccgcagc
     2881 ctgtgcctcc tccttcttgc ccgcgcgcca caactcgccg atgcgctgtg ccgcctcggg
     2941 gaagccgcgg cgcgccatcg cgtcgcggtg gtagttgtgc gtctcgcttc ccatgccacc
     3001 gacgtacatc gcggtcagcg gccgcatcgc gtcgagcgcg ccttgcacgt cgtcggtgat
     3061 gttgacggcc gcgccggtga agacctcgaa gccgtccttc tctcgttcgc gcatcgcggc
     3121 cggcacgccg tcggggccga ggcccatcgg cagccacccg tcgaacagct cggccgccaa
     3181 tgcgttgttc ttcgggccgc cggatgccag ccagatcggg atcggcgcga ccggatgcat
     3241 gatcgacttc agcgccttgc cctggccgag cgcgcccggg ccgcgatacg gcagttgcag
     3301 ctccggaccg tcgtgcacga cgggctcctc gcggtcgagc accttgcgca tgatcgtgac
     3361 gtagtcgcgc aggcgcag
//
LOCUS       DASUGA010000714         4588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_213413, whole genome shotgun sequence.
ACCESSION   DASUGA010000714 DASUGA010000000
VERSION     DASUGA010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213413"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1563)
                     /locus_tag="VFX21_14155"
                     /pseudo
     CDS             complement(1..1563)
                     /locus_tag="VFX21_14155"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            1604..2032
                     /locus_tag="VFX21_14160"
     CDS             1604..2032
                     /locus_tag="VFX21_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5097165.1"
                     /translation="MQFEHDETINAPADVVWAAYADVERWPDWADSFRSVRYVEGDAL
                     VLGARVRIEQPKLPKAEWTVTDVQPGRAWTWVSKAPGIETTAVHDVIAGGAQTRVHTT
                     ITQRGPLGAVFGRLYAKLTRSYMAMEGAGLKRRSEARASA"
     gene            2062..2553
                     /locus_tag="VFX21_14165"
     CDS             2062..2553
                     /locus_tag="VFX21_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX5097166.1"
                     /translation="MLAAVIDAVGRNGLGSRSLRDLAGDAGTSHRMLIHHFGSREGLL
                     VAIVSEVERRQAEVLRPLPPDPAEALSAVWNSVRSPTLWPFERLFFECYARGAQGEAP
                     FDRLLPALVENWLAGRRGRDRDQARLALAVVRGLLLDLVATEDKEAVNRALKEFLVLL
                     RAA"
     gene            2566..3849
                     /locus_tag="VFX21_14170"
     CDS             2566..3849
                     /locus_tag="VFX21_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011209117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097167.1"
                     /translation="MTTRPSIAFDANFRLQDLSRQTLAILGREWLLHGHLQDRIAMPL
                     VLTGRSRDVMEAVAIDEWMGASPIYSKRVQRALRFEGDGVDTIMKNLQLDIGSPHHFM
                     DFRCTVHDHDHGEFFLAHCGALMDVEPMGEDFVRGMCHAIEDPTFDATAGATNPRAQM
                     RPVHRPPRVPADRQPHCHWTVTIDPAHEPASVHPNLAVVTQSLVPGCAVEDPGDGTEP
                     GGWDDYAGPFDTDFALEDLSQRALVVALQEVALQSHILVRSLCVSIANRLGADVAREI
                     APNVAVGLAGLTAERLTAAFDFGADAPGLAALLTVHPMFWPRTYVQPVIAVDGNRVRF
                     ALRDESTIFAEADDYTWLASVPGIGDRILLALAQGFDRRAQVATLPPGPGERCAYELT
                     IDPATAPAREQREVSLGKISTGAAFVLEPRRPVRI"
     gene            4060..>4588
                     /locus_tag="VFX21_14175"
     CDS             4060..>4588
                     /locus_tag="VFX21_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011758063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097168.1"
                     /translation="MHRLRWLVGGVVLTVALGTAAAGTALAGGRSDHPPSPKVPNAQF
                     DRDMRKLWEDHITWTRLFIVSASADLPDLQATTDRLLRNQTDIGDAIKPFYGDAAGNQ
                     LTALLRTHILTAADIVAAAKAGDDAAVDEATTAWYANADDIAAFLSQANPKQWPFAEM
                     RTMMRDHLDLTLAEAV"
BASE COUNT          712 a         1590 c         1577 g          709 t
ORIGIN      
        1 gtagcgcacc tcggggcggc gggcggcgag acggtcgacg tcgaggacgt tcacgtcggc
       61 cttgaaaccc ggctgcaaca cgccgcggtc gagcaggccg acggtgcgcg ccgtcgcctg
      121 cgtctggcgg tgcaccaaga actccaacgg caggcggccg tggtcgcgat cgcgtcccca
      181 caacgcgagc agcgtcgtcg ggaagctcgc atcgcagatg gtgccgacgt gcgcgccgcc
      241 gtcgccgagc ccgacaacgg tgtggttcgc ggcgagcatc tcgcccgcgg cgtcgaggtt
      301 gccgtcgtcg tagttgagga cgggccggta gaggaagccg cgcccgccgt cggcgagcag
      361 gatgtcgtac gcaaggtcga acgggtcgcg accttcccgc cgggcgcgcg cctcgatgct
      421 cgcctccggt agcggttagt agtcgggcgg atcgccgagc tcgaacatgt ggtcgaagcc
      481 accgagcatg cggccgccgg gtccgctgcg cgccgcttcg tcggcctcct tgacgcgcgc
      541 gcggaactcg ggctcggaca tgatcgcgac gcgttcggcg aggggcagtg ccgcgatctc
      601 gcgatacacc gggttcgcga gcagcggatg cagcgtgcac tcgaggccga gcagcaaccc
      661 cacggcgcgc ggcgcaacct gggcgcgcat cgtcacgccg tcggcattcg cgccggccaa
      721 cagctcgagc tggcgccgcc acgagtcgcg cttggtctgc agcagcgtga agctgagcgg
      781 ccgccccgaa gactccgaca tggaacggaa cagcgcgaac tcggcgtcga cgtcgttgaa
      841 gtcggaaacc gcttgcagca ctcctttgcc ggtcgcgccg atcgccttcg cgatgccgac
      901 cagttcgtcg gcctcggcgg tcagcgtcgg cgtgaactcg ccgcggctgc tgcggtggtt
      961 catcgtgcgc gaggtcgaga aaccgagcgc gcccgactcg atggcttcgg ccgcgatctt
     1021 cgccatcaac tcgatgtctt cgggtgtcgc ggcctcgcgt tgcgcgccgc gctcgcccat
     1081 cacatgcagg cgcagcgcgg cgtgcggcac ttgcagcgcg acgtcgacat cgaacggacg
     1141 gccgtcgacc gcgtccatga actcggcgaa ggaccgccag ttccagagga ggccttcgtg
     1201 cagcgcgacg ccgggaatgt cctcgacgcc ttccatcagc tcgatgagac ggtcgtgatc
     1261 ggcgtcgtgc acgggcgcga agccgacgcc gcagttcccg gcgacgaccg tggtcacgcc
     1321 gtgccatgac gacgggatca tgcgttcgtc ccacgacgcc tggccgtcgt aatgcgtatg
     1381 gatgtcgacg aagccgggcg tgacgagcag cccgtcggcc tcgatcgtgc ggttcgcggc
     1441 accggagacg gtgccgacgt cggtgatgac gccgccgtcg atggcgacat cggcgcgccg
     1501 gcgcggcgcc cccgtgccgt cgacgacctc accaccacga atgacgagct cgtggtcagt
     1561 cacctcttga tcctacgacc gcagtcgatg taccatttgg tacatgcagt tcgaacacga
     1621 tgagacgatc aatgcgccgg ccgatgtggt gtgggctgcc tacgccgacg tggaacgctg
     1681 gcccgactgg gcggattcgt ttcgctccgt ccggtatgtc gaaggcgacg cgctcgtgct
     1741 cggtgcgcga gtgcgcatcg agcagccgaa actcccgaag gccgagtgga cggtgaccga
     1801 cgtccagccc ggtcgcgcgt ggacctgggt gtcgaaggcg ccgggcatcg agacgacggc
     1861 ggtgcacgac gtgatcgcgg gcggcgcgca gacgcgcgtg cacacgacga tcacccagcg
     1921 cgggccgctg ggtgccgtct tcggccgcct ctacgcgaag ctcacgcgct cgtacatggc
     1981 gatggagggt gccggcctca agcggcggtc cgaggcgcgc gcctccgcgt gacgacaacc
     2041 gagacgacac cgcgagacgc gctgctcgcg gccgtgatcg acgcggtggg ccgcaacggt
     2101 ctgggctcgc gctcgctgcg cgacctagcc ggcgacgcgg gcacgagcca ccgaatgctg
     2161 atccatcact tcgggtcgcg cgagggattg ctcgtcgcga tcgtctccga agtcgagcgc
     2221 cggcaagcgg aggtgttacg accgttgccg cctgatccgg cggaggcgct cagtgcggtg
     2281 tggaacagcg tcaggagtcc gaccctgtgg ccgttcgagc gcctgttctt cgagtgctac
     2341 gcgcgcggcg cccaaggcga agcgccgttc gaccggttgc tccccgcgct cgtcgagaac
     2401 tggctggcgg gccgccgcgg gcgcgatcgg gaccaggccc ggctcgcgct cgccgtcgtg
     2461 cgcggcctcc tcctcgacct cgtcgccacc gaggacaagg aagccgtcaa ccgggcgttg
     2521 aaggagtttc tcgtgctgtt gcgcgccgcg taacgttcgc ggccgatgac gactcggccg
     2581 agcatcgcgt tcgacgcgaa cttccgtctg caagacctct cgcggcagac gctcgcgatc
     2641 ctcgggcgcg agtggttgct gcacggccac ctccaggacc ggatcgcgat gccgctcgtg
     2701 ctcaccgggc gatcgcgcga cgtcatggaa gcggtcgcga tcgacgagtg gatgggcgcg
     2761 agcccgatct attcgaagcg ggtgcaacgc gcgctgcgct tcgaaggtga cggtgtcgac
     2821 accatcatga agaacctgca gctcgacatc ggatcgccgc accacttcat ggacttccgc
     2881 tgcacggtgc acgatcacga tcacggcgag ttcttcctcg cgcattgcgg tgcgctgatg
     2941 gacgtcgagc cgatgggcga agacttcgtc cgcggcatgt gccacgcgat cgaggacccg
     3001 accttcgacg ccaccgcggg cgcgacgaac ccgcgcgcgc agatgcgccc ggtccaccgt
     3061 cccccgcgcg tgcccgccga ccggcagccg cactgccact ggacggtcac gatcgatccc
     3121 gcgcacgagc ccgcgtccgt gcaccccaac ctcgcggtcg tcacccagtc gctcgtcccg
     3181 gggtgcgccg tcgaggatcc gggtgacggt accgagcccg gcgggtggga cgactacgcc
     3241 gggccgttcg acaccgactt cgcgctcgaa gacctgtcgc agcgcgcgct cgtcgtcgcg
     3301 ctccaagaag tcgcgttgca gagccacatc ctcgtgcgct cgctgtgcgt ctcgatcgcc
     3361 aaccgcctcg gcgccgacgt cgcgcgcgag atcgcgccga acgtcgcggt ggggctcgcg
     3421 ggcctgaccg cggaacggct caccgccgcc ttcgacttcg gggcggacgc ccccggactc
     3481 gccgcgctgc tcaccgtgca cccgatgttc tggccgcgca cgtacgtgca gccggtcatc
     3541 gccgtcgacg gcaaccgcgt gcggttcgcg ctgcgtgacg agtcgacgat cttcgccgaa
     3601 gccgacgact acacgtggct cgcgtcggtg cccggcatcg gcgatcgcat cctgctcgca
     3661 ctcgcgcagg gcttcgaccg ccgggcgcag gtggccacgt tgccacccgg gcccggcgaa
     3721 cgatgcgcgt acgagctgac gatcgacccg gccaccgcgc ccgcgcgaga gcagcgcgaa
     3781 gtgtcattgg gcaagatcag caccggcgcc gcgttcgtgc tcgaaccgcg gcgaccggtg
     3841 cgcatctgag ctccgtcact tcttcttgtg cgtgagcacg ctgatcgtcg actgctcgac
     3901 cggatcatga tcgttcgcat gaactgatgg aacgccgatc cgggccgcgg ctcctgagta
     3961 ggtgctcctc aatttctttc taaaaacaat agatcttgac gttatcgctc gaccgttcta
     4021 acgttactct ccattgacga tagaacgaga ggggatctca tgcatcggct ccggtggttg
     4081 gtagggggcg tcgtcctgac ggtggcgctg ggcaccgcgg cggcggggac cgcgctcgcg
     4141 ggcgggcggt cggatcaccc gccgtcaccg aaggtcccga acgcacagtt cgaccgggac
     4201 atgcgcaagc tgtgggagga ccacatcacg tggacgcgct tgttcatcgt cagcgcgtcc
     4261 gccgacctcc ccgacctgca ggcaaccacc gaccggttgc tgcggaacca gaccgacatc
     4321 ggtgacgcga tcaaaccctt ctacggcgac gcggcgggca atcaactcac cgcgctgctg
     4381 cggacgcaca tcctgaccgc cgccgacatc gtcgcggccg cgaaggccgg tgacgacgcc
     4441 gcagtcgacg aggcgaccac ggcctggtac gcaaacgccg acgacatcgc cgcgttcttg
     4501 tcgcaggcga atccgaagca gtggccgttc gcggagatgc gcacgatgat gcgtgaccat
     4561 ctcgacctca cattggcgga ggccgtcg
//
LOCUS       DASUGA010000715         2232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_419643, whole genome shotgun sequence.
ACCESSION   DASUGA010000715 DASUGA010000000
VERSION     DASUGA010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419643"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VFX21_14180"
     CDS             complement(<1..779)
                     /locus_tag="VFX21_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5097169.1"
                     /translation="MAVISIAGLVKTFGSTKALDGLDLVVEPGEVHGFLGPNGSGKTT
                     TIRVLLGLLRADRGSVTLLDGDPWRDAVALHRRLAYVPGDVTLWPKLSGGEVIDLLGR
                     LRGDLDPVRRDELLERFELDPTKKARTYSKGNRQKVGLVAALASRAELLILDEPTSGL
                     DPLMEAVFQDCIREVKAEGRTVLLSSHILAQVEALCDRVSIIRAGRTRQSGTLRELRG
                     MTRTSIVAELAGSPDGISALPGVHGLEVDGSRVRFDVDTVHL"
     gene            complement(809..>2232)
                     /locus_tag="VFX21_14185"
     CDS             complement(809..>2232)
                     /locus_tag="VFX21_14185"
                     /inference="COORDINATES: protein motif:HMM:NF039585.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HEX5097170.1"
                     /translation="DLPDHAYWERWFPADWVSEMREQIRLWFYAQLFMSVALTGRAPY
                     RQVLGYEKMLDEHGVEMHGSLGNMIDAPEAFAQMGADVMRWMYCAQPPSQNLLFGFGP
                     AYEIQRKLLTLWNSVSFFVQYANIAEFTPAFADLDDDAAGGAVLDRWLVARTRHLVAD
                     ATAAYDEFLTVNVIRAYEEFVNDLSKWYVRRSRRRFWDGDVDALRTLWTSIVHSLRVI
                     APVMPFLAERLWQDLVRDIDADAPASVFLAGWPELGPADDELLLAMTAVRDVIDLGRE
                     ARAAVKLRNRQPLHRIVVEGADRAQAYAQEIKDELNIKDVEFGTIDATELRVRPDLKV
                     LGPRLGKEVGKVRAALEAGNFDVLPDGGFRAAGHDLTAADVLVERTSKEGWAVAAKDG
                     VSIAIDTSLDDALVLEGRVNDLIHSINSARKEAGLELTDRIHLRLARSDEALLAHKDR
                     IAAEVLAVSVELGDADEFQFTRV"
BASE COUNT          343 a          756 c          788 g          345 t
ORIGIN      
        1 aggtgcacgg tgtcgacgtc gaagcgcacg cgcgagccgt cgacctcgag gccgtggacg
       61 ccgggcagcg ccgagattcc gtccggcgaa cccgcgagct cggcgacgat cgacgtgcgc
      121 gtcatgccgc gaagctcgcg caacgtgccg ctctgccggg tgcgaccggc gcggatgatg
      181 ctcacgcggt cgcacagtgc ctccacttgg gcgaggatgt ggctcgacag cagcaccgtg
      241 cggccctcgg ccttcacttc gcggatgcag tcctggaaca ccgcttccat cagggggtcg
      301 agacccgacg tcggctcgtc gaggatcagc agctccgcgc gcgacgcgag cgccgcgacg
      361 agaccgacct tttgacgatt gcccttcgag taggtgcgcg ccttcttcgt cgggtcgagc
      421 tcgaatcgtt cgagcagctc gtcgcgccga acgggatcga gatcgccgcg caaccggccg
      481 aggagatcga tgacctcgcc gccactcagc ttcggccaga gggtgacgtc gccgggcacg
      541 taggcgaggc gtcggtgcag cgcgaccgcg tcgcgccatg ggtcgccgtc gagcagcgtg
      601 accgagccgc ggtccgcccg gagcagcccg agcagcacgc ggatcgtcgt ggtcttcccc
      661 gagccgttcg gcccgaggaa gccgtgcacc tcaccgggct cgacgacgag atcgaggccg
      721 tccaatgcct tcgtggaccc gaaggtcttg acgagacccg cgatcgagat gacggccatg
      781 cggagagtct tccaccgggc gcgcgagatc agacgcgcgt gaactggaac tcgtcggcgt
      841 cgccgagctc gacgctgacc gcgagcactt ccgccgcgat ccggtccttg tgcgcgagca
      901 acgcctcgtc ggaccgggcc aggcgcaggt ggatgcgatc ggtgagctcg agaccggctt
      961 ccttgcgcgc gctgttgatg ctgtggatca gatcgttcac gcgcccttcg agcaccaagg
     1021 cgtcgtcgag tgacgtgtcg atcgcgatgc tgacgccgtc cttcgccgcg acggcccaac
     1081 cctccttcga cgtgcgttcg acgagcacgt cggcggcagt gaggtcgtgg ccggcggcgc
     1141 ggaacccgcc gtcggggagg acgtcgaagt tgccggcttc cagcgcggcg cgcaccttgc
     1201 cgacttcttt gcccaggcgc ggcccgagca ccttgagatc gggccgcacg cgcagctccg
     1261 tcgcgtcgat cgtgccgaac tcgacgtcct tgatgttgag ctcgtccttg atctcctgcg
     1321 cgtacgcctg ggcgcggtct gcgccttcga cgacgatgcg gtgcagcggc tggcggttgc
     1381 gcagcttgac cgcggcgcgc gcctcgcgtc cgaggtcgat gacgtcacgc actgccgtca
     1441 tcgcgagcag gagctcgtcg tcggccgggc cgagctcggg ccagccggcg aggaagaccg
     1501 acgccggcgc gtcggcgtcg atgtcgcgga cgaggtcttg ccacagccgt tcggcgagga
     1561 acggcatcac cggtgcgatg acgcgcagcg agtggacgat cgacgtccac agcgtgcgca
     1621 aagcgtcgac gtcgccgtcc cagaagcggc ggcgcgagcg gcgcacgtac cacttcgaca
     1681 ggtcgttgac gaactcctcg tacgcgcgga tcacgttgac ggtgaggaac tcgtcgtagg
     1741 ccgccgtcgc gtccgcgacg aggtggcggg tgcgggcgac gagccagcgg tcgagcaccg
     1801 caccgccggc cgcgtcgtcg tcgaggtcgg cgaacgccgg cgtgaactcg gcgatgttcg
     1861 cgtactggac gaagaacgag acgctgttcc acaacgtcag cagcttgcgc tggatctcgt
     1921 acgcgggccc gaagccgaac aggaggttct ggctcggcgg ctgcgcgcag tacatccagc
     1981 gcatgacgtc cgcgcccatc tgcgcgaatg cttccggcgc gtcgatcatg ttgccgagcg
     2041 agccgtgcat ctccacgccg tgctcgtcga gcatcttctc gtagcccagc acctgccggt
     2101 agggcgcgcg tccggtcagc gcgaccgaca tgaacagctg ggcgtagaac cagagcctga
     2161 tctgctcgcg catctcggag acccagtcgg ccgggaacca gcgctcccag taggcgtggt
     2221 cggggaggtc gg
//
LOCUS       DASUGA010000716         6502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1603439, whole genome shotgun sequence.
ACCESSION   DASUGA010000716 DASUGA010000000
VERSION     DASUGA010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6502
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603439"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2335
                     /locus_tag="VFX21_14190"
     CDS             <1..2335
                     /locus_tag="VFX21_14190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX5097171.1"
                     /translation="RRAPALAVLCIGAAFVAGSLAAPSVSAATPPFSAVGSVGQVYVT
                     GLAAAAQASLVNASGQTLQTRAANALGGLLFRNVAPATGYRVRRVADGKTSAQLTVRS
                     GKASPWNPDVYNQSIAPGGYSYLTTRDGTQLAINVHVPTSPAGQPGLPPGTPIPSGPA
                     YAPPYPTLIEYSGYAYADPAGPTSGIAVLANLMGFAVVDVNMRGTGCSGGAFDFFEPL
                     QSLDGYDVIETIARQPWVRDHKVGMMGISYGGISQLFTARLRPPSLAAISPLSVIDAT
                     ASTLYPGGILNTGFAVAWAEERQEQALPAGPDSGQPYAYKRIQEGDTTCAKNQVLHPE
                     ATDLRAKIDANAHYVPQVADPLDPVTFVDKINVPVFMSCQWQDEQTGGHCPELVRKFT
                     GTNQKWFTFTNGAHIDSLDPATFNRWYDFLMLFVARRAPMQNAAIPRAAAPVIYQAAM
                     GLPPTDVVTLPPDPIQAIPTYDAALAAFNQLPRVRVLFENGAGSQLGASSNPGDPYPA
                     FEKSFSSLPVSGTTARTWYLGPAGALGDQPPAGQGANWFRAKANALPPTDYGSNTGTG
                     GLWGNASQWDWRWRQNPAGSAVSYVTAPLSANTAVIGSGSVNLWVRSSTPDVDLLATV
                     SEVRPDGKETFVQNGYIRASERKLSTTTNNIFKRPSTALEPIPSFTAADAAPMPAGQF
                     AKVVVPLYYQGHAYRAGSRIRVTIAAPNGTQPIWSFAETQPAGTTSKVFIAFSPSMPS
                     SVILPVVPNVTVPTGLPACPSLRNQPCRPYQAIVNNT"
     gene            complement(2426..3943)
                     /locus_tag="VFX21_14195"
     CDS             complement(2426..3943)
                     /locus_tag="VFX21_14195"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HEX5097172.1"
                     /translation="MDSLLARLFEGDDDSFYARLAAHGHEIITDEDFAACYVSGRGRP
                     SIPPSLLMRAVLCQIRDDVSDREAARRAAKDLDWKRALGIEADEVPFHHTTLSVFRSR
                     LLVNDADGVVLTKTVERAVAAGLFRKRVLAIADSTGVMGAAAVADTYELIRQAIAKLV
                     GAAGGPKLLPSRLRRRARTYVRGKATIDWADASARRTELDALVTTARELLAATADNDD
                     CSEARELLGRIIDQDVDEDPDDGNGPKIRHGVAPDRVVSVVDPEMRHGRKSPSKRVDG
                     YKAHVLTDHDHEFVLAVSATPANVADGPEAAGLVQAAHDAGVRVREMLGDTAYGDGDT
                     RVAVEAVGATVTAKTQPPATTGKFLKTDFTIDPTIPSARCPAGEETTAVFHQRDHKRR
                     PLIALQFSAEVCDTCSLRERCTTSPKGRRVVLNFHEARLQAARAEQKRPSTRRKLKRR
                     ALVERKLAELKMHGMGKARYRGQRKVLVQIRLTATLVNLKKLFSLERPTLDACHA"
     gene            4108..5577
                     /locus_tag="VFX21_14200"
     CDS             4108..5577
                     /locus_tag="VFX21_14200"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5097173.1"
                     /translation="MRPLLVAAQVALVGVVFGVGVGFLIDAAMADLLRSFQPLPVWDT
                     GFQPAVFVRGAALGLLLPFLATLLPVWRAVRVSPIEAISTAHRAASGGLAPAVRRIPL
                     PGSSVAQLPFRNVLRAPRRTLLTAFGIAAAIATLVGVVGVVDSFLATIDRGDTEIRGD
                     QPDRVTVTLDSYVPADSAPVDAVAGAASVGASEAGLVVPGALDPGRVDEIEILLSATD
                     FDSALWRPTAIEGALSTDEPGLVISEKAAGDLGVSIGENVRVRLPRREGLTGYSYFET
                     DLPVLAIHPNPYRFVAFIDRRWAGVMNLEGVVNVVQVIPAAGSSPADVQRELFGTDGV
                     ASVEPVAVLAQAIRDRLGETLGIFDVIQAVVLLLALLIAFNSTAINMDERARENATMF
                     AFGLRARAVMGVAVTESVIVGVLGTVLGVLGGRILLGWLVEVLIPETFPDISIEVALA
                     PSTIVTAIGFGVLAVAVAPLFTMRRLRRMDISSTLRVVE"
     gene            5577..>6502
                     /locus_tag="VFX21_14205"
     CDS             5577..>6502
                     /locus_tag="VFX21_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013415452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX5097174.1"
                     /translation="MPRETAGRDDALTCGLTAAEAARRRAHDGPNRLPAPKARPVWRR
                     LLSEFVHFFAIMLWIASALAIVAGLPQLAIAIAIVVVLNGVFAFAQEYRAERAAERLR
                     DLLPRRATVLRDGTAREIDADELVVGDVVLLDAGDRVSADLDVHRAHQCFVDTSMLTG
                     ESVPESVDAGARLHAGSFLVEGEATAVVVATGARTRLADIAALTTAAGRHASPLATEL
                     ARVVRTIAAIALGVGVGFFVLAVLIGTPANDGFVFAIGVTVALVPEGLLPTVTLSLAI
                     GAQRMAKRQALVRKLEAVETLGSTTFVCTDKTG"
BASE COUNT          980 a         2284 c         2152 g         1086 t
ORIGIN      
        1 gcggcgcgcg cccgcgctcg ccgttctgtg catcggcgcg gcattcgtcg cgggttccct
       61 cgccgcgccg agcgtgtcgg ccgcgacgcc acccttcagc gcggtcggca gcgtcggtca
      121 ggtgtacgtc accggtctcg cggccgcggc gcaggcgtcg ctcgtcaatg catcgggaca
      181 gacgctgcag acgcgagcgg cgaacgcgct cgggggactg ctgtttcgga acgtcgcgcc
      241 ggcgaccggt tatcgcgtcc ggcgcgtcgc cgacggcaag acatcggcac agctcacggt
      301 gcgctccggc aaggcgtcgc cgtggaaccc ggacgtctac aaccaatcga tcgcgcccgg
      361 cggctacagc tatctcacca ctcgtgacgg cacgcagctc gcgatcaacg tgcacgtccc
      421 gacgagcccg gccggccaac ccgggcttcc gcccggtacg ccgattccga gcggtccggc
      481 gtatgcgccg ccgtacccga cgctgatcga gtactccggg tacgcgtacg cggatcccgc
      541 gggaccgacg agcggcatcg cagtgctcgc gaacctgatg ggtttcgccg tcgtcgacgt
      601 gaacatgcgc ggcaccggat gctcaggtgg cgcgttcgac ttcttcgaac cgctgcagag
      661 cctcgacggc tacgacgtga tcgagaccat cgcgcggcaa ccgtgggtgc gcgatcacaa
      721 ggtcgggatg atgggcatct cgtacggcgg catcagccag ctcttcaccg cgcgtctccg
      781 cccgccgagc ctcgcggcga tctcaccgct gtcggtcatc gacgcgaccg cgtcgacgct
      841 ctatccgggc ggcatcctca acaccggctt tgccgtggcc tgggccgagg agcgtcagga
      901 gcaagcgctc ccggccggtc ccgacagcgg ccagccctac gcgtacaagc gcatccaaga
      961 gggcgacacc acctgcgcga agaaccaggt gctccacccc gaggcaaccg acctgcgcgc
     1021 gaagatcgac gccaacgcgc actacgtgcc gcaggtcgcc gatccactcg atcccgtcac
     1081 gttcgtcgac aagatcaacg tgccggtgtt catgtcgtgc cagtggcaag acgaacagac
     1141 cggcgggcac tgccccgaac tcgttaggaa gttcaccggc accaaccaga agtggttcac
     1201 gttcaccaac ggcgcacaca tcgactcgct cgatcccgcg acgttcaacc gttggtacga
     1261 cttcttgatg ttgttcgtcg cgcggcgcgc accgatgcag aacgcggcca tcccgcgcgc
     1321 ggcggcgccc gtcatctacc aggcggcaat gggactgccg ccaaccgatg tcgtcacgct
     1381 cccgcccgac cccatccagg cgatcccgac gtatgacgcg gcgctggccg cgttcaatca
     1441 gctgccgcgc gttcgcgtgt tgttcgagaa cggcgccggc tcgcagctcg gcgcgagctc
     1501 gaaccccggc gatccgtatc cggcgttcga gaagtcgttc tcttcgttgc cggtgtcggg
     1561 cacgaccgcg cgcacctggt acctcgggcc cgcgggcgcg ctcggcgacc aaccacccgc
     1621 cgggcagggc gccaactggt tccgggccaa ggcgaacgcg ctcccgccca ccgactacgg
     1681 gagcaatacc ggcaccggcg gcctgtgggg caatgcatcg caatgggatt ggcgatggcg
     1741 gcagaaccct gcgggcagcg cggtctcgta cgtgaccgcg ccgctctcgg ccaacaccgc
     1801 cgtcatcggg agcggctccg tgaacctgtg ggttcgctcg tcgacgcccg acgtcgacct
     1861 gctcgcaacc gtcagcgaag tgcggcccga cggtaaggag accttcgtcc agaacggcta
     1921 catccgcgcc agcgagcgca agctctcgac gacgacgaac aacatcttca agcgaccgag
     1981 caccgcgctc gaaccgatcc ccagcttcac cgcagccgac gcggcgccga tgccggcggg
     2041 gcagttcgcc aaggtggtcg tcccgctcta ctaccagggt cacgcgtacc gcgcggggtc
     2101 gcgcattcgc gtgacgatcg ccgcgccgaa cggcacgcag ccgatttggt cgttcgcaga
     2161 gacacaaccg gccggcacca cgtcgaaggt gttcatcgcg ttctcaccgt cgatgccgtc
     2221 gagtgtcatc ctgccggtgg tgcccaacgt caccgtgccg acggggctgc ccgcgtgccc
     2281 gagcctgcgc aaccagccgt gccggccgta tcaggcgatc gtgaacaaca cctaggtggc
     2341 tgccgaagaa gtgcccttcg gcgagtggtt cggtgggctg atgtcggggt tcgggggctg
     2401 tgtggtcggc gatgcgctcg ggcgatcatg catggcaggc gtccagggtc ggtctttcga
     2461 ggctgaagag cttcttgagg ttcacgagcg tggcggtgag gcggatctgc accagcactt
     2521 tgcgttgtcc gcgataacgg gccttgccca tcccgtgcat cttcagctcg gcaagtttgc
     2581 gttccacgag cgctcgtcgt ttgagcttgc gtcgggtcga tggtcgtttc tgttcggcac
     2641 gcgcggcctg caggcgcgcc tcatggaagt tgagcacgac gcgtcgtccc ttcgggctgg
     2701 tcgtgcaacg ctcgcgcagc gaacacgtgt cacagacctc ggccgagaac tgcaacgcga
     2761 tgagcgggcg ccgtttgtga tcacgctggt ggaacacggc ggtggtttcc tcaccggccg
     2821 ggcagcgcgc ggacgggatg gtcggatcga tcgtgaagtc ggtcttgagg aacttgccag
     2881 tcgtggccgg gggctgggtc ttcgcggtca ccgtcgctcc gaccgcttcg accgcgacgc
     2941 gggtgtcgcc atcgccatag gcggtgtcgc ccagcatctc gcgcacccgc acgcccgcat
     3001 cgtgtgcagc ctgcacaaga cccgcggctt cgggcccgtc cgcaacgttc gctggtgtcg
     3061 cgctgaccgc gaggacgaac tcgtgatcat ggtcggtgag cacgtgcgcc ttgtaaccat
     3121 ccacccgctt cgatgggctc ttgcggccgt gccgcatctc cggatcgacg accgacacca
     3181 cccgatcggg cgcaacaccg tgacggatct tcgggccgtt cccgtcgtcg ggatcctcat
     3241 caacgtcttg gtcgatgatg cggcccaaca gttcccgagc ctcgctgcag tcgtcattgt
     3301 cggcggtcgc cgcgagcagt tcgcgggcgg tcgtgaccaa cgcgtcgagc tcggttcgac
     3361 gcgcagacgc gtcggcccag tcgatcgtgg ccttgccccg cacatacgtc cgggctcgtc
     3421 tccgcagccg ggacggcaac aacttggggc caccagcggc gccgacaagt ttcgcgatcg
     3481 cttggcggat gagttcgtag gtgtccgcca ccgcggcggc gcccatcacc ccggtcgagt
     3541 ccgcgatcgc caacacccgc ttgcggaaca gccccgcggc gacggcccgc tcgacggtct
     3601 tcgtcaacac gacaccatcg gcgtcgttga ccagcaaccg ggaccggaac accgacaagg
     3661 tcgtgtgatg gaacggcacc tcgtcagctt cgatccccaa cgcccgcttc caatcgagat
     3721 ccttcgccgc gcgccgggcg gcctcccgat ccgagacgtc gtcgcggatc tggcacaaca
     3781 ccgcccgcat caacaacgac ggaggaatcg acggccggcc ccgacccgac acgtaacacg
     3841 cggcgaagtc ctcatcggta atgatctcgt gaccgtgcgc ggccagccgc gcgtagaacg
     3901 agtcgtcgtc accctcgaac agccgggcca gcagcgaatc caccccgagg aggctccctt
     3961 ggcggtcagt cgtcccgagc atcatgacct cgcagcgatc ccatcgcacg agcatgacca
     4021 aaccgagccc gaatcgcgag caccctcagt ccacaacccg ccgacttttt cggcagccac
     4081 ctaggcgaac cgcgcgggtc gatcgcggtg cgaccactgc tcgtcgcggc gcaggtcgca
     4141 ctggttggtg tcgtcttcgg tgtcggcgtg ggctttctca tcgacgccgc gatggccgat
     4201 ctgctgcgtt cgttccagcc gctccccgtc tgggacaccg ggtttcaacc ggccgtcttc
     4261 gttcgcggcg cggcactggg tctgctgctg ccgttcctcg cgacactgct ccccgtgtgg
     4321 cgggcagtgc gcgtctcgcc gatcgaggcg atcagcacgg cgcaccgcgc cgcgagcggc
     4381 ggccttgcgc ctgcggtgcg ccggatcccg ctgccgggtt cgagtgtcgc gcagctcccg
     4441 ttccgcaacg tgctgcgcgc cccgcgccgg acgctgctca cggcattcgg gatcgccgcg
     4501 gcgattgcga cgctcgtcgg tgtcgtcggc gtcgtcgact cgttcctcgc gacgatcgat
     4561 cggggtgaca ccgagattcg cggtgatcag cccgaccgtg tcaccgtcac gctcgactcg
     4621 tacgtgccgg cggactccgc gccggtcgac gcggtggccg gcgcggcgtc ggtcggtgcg
     4681 tcggaagccg gtctcgtggt gcccggcgcg ctcgaccccg ggcgcgtcga cgagatcgag
     4741 atcttgctgt cggcgacgga cttcgacagt gcactgtggc gtccgactgc gatcgaaggg
     4801 gcgctgtcaa ccgacgaacc gggtctcgtc atctcggaga aagcggccgg cgacctcggc
     4861 gtgtcgatcg gcgagaacgt gcgcgtccgc ctcccgcgac gcgagggcct caccggctac
     4921 agctatttcg agacggatct gcccgtgctc gcgattcacc cgaacccgta ccgtttcgtc
     4981 gccttcatcg atcgtcgatg ggcaggcgtg atgaacctcg aaggtgtcgt gaacgtcgtg
     5041 caggtcattc cggccgcggg ttcgtcgccg gcggatgtgc agcgcgagct gttcggcacc
     5101 gatggtgtcg cttccgtcga gccggttgcc gttcttgcgc aggcgattcg cgatcgcctc
     5161 ggcgagacgc tcggcatctt cgacgtcatt caggcggtcg tgctgttgct cgcgctcctg
     5221 atcgcgttca actcgaccgc gatcaacatg gacgaacgcg cacgggagaa cgccacgatg
     5281 ttcgcgttcg gactccgcgc tcgcgcagtg atgggcgtcg cggtgaccga gagcgtcatc
     5341 gtcggcgtgc tcggcacggt gctcggtgtg ttgggcggcc gcatcctcct ggggtggctg
     5401 gtcgaagtgc tgatccccga gacgttcccc gacatcagca tcgaggtcgc gctcgcgccg
     5461 agcacgatcg tgacggcgat cggcttcgga gtgctggccg tggccgtggc gccgctcttc
     5521 acgatgcgcc ggctacgtcg gatggacatt tcgtcgacgc tgcgggtcgt cgaatagtgc
     5581 cgagggaaac cgcgggacgc gacgatgcac tgacgtgcgg cttgacggcc gccgaagcgg
     5641 cgcggcgccg tgcgcacgac ggtccgaacc gattgcccgc gcccaaggcc cggccggtct
     5701 ggcggcgcct gctctccgag ttcgtgcact tcttcgcgat catgttatgg atcgcgagcg
     5761 cactcgcgat cgtcgccgga ctcccgcagc tcgcgattgc gatcgcgatc gtcgtcgtgc
     5821 tcaacggcgt cttcgccttc gcgcaggagt accgggccga gcgtgcggcg gaacggctcc
     5881 gcgatctgct cccacggcgc gcaaccgtgc tgcgcgacgg aaccgcacgc gagatcgacg
     5941 ccgacgagct cgtcgtcggt gacgtggtgc tgctcgacgc gggcgaccgc gtctccgccg
     6001 acctcgacgt ccaccgcgcg caccagtgct ttgtcgacac gtccatgctc acgggggaga
     6061 gcgtccccga atccgtggac gccggcgccc gtctgcacgc gggctcgttc ctggtcgaag
     6121 gcgaggcgac tgcagtcgtc gtcgcgaccg gagcccgaac gagacttgcc gacatcgccg
     6181 cgctgacgac cgccgccggc cggcatgcga gcccgctcgc gacggagctc gctcgtgtcg
     6241 tgcggaccat cgcggcgatc gcgctcggcg tcggggtcgg cttcttcgtc ctcgcggtcc
     6301 tcatcgggac accggccaac gacggattcg tgttcgcaat cggtgtcacg gtcgcactcg
     6361 tccccgaagg actcctaccg accgtgacgc tgtcgttggc gatcggcgcg caacgcatgg
     6421 cgaagcgcca agcactcgtg cgcaagctcg aggcggtgga gacgctcggc tcgaccacat
     6481 tcgtctgcac cgacaagacc gg
//
LOCUS       DASUGA010000717         6247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1324187, whole genome shotgun sequence.
ACCESSION   DASUGA010000717 DASUGA010000000
VERSION     DASUGA010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324187"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VFX21_14210"
     CDS             <1..1005
                     /locus_tag="VFX21_14210"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5097175.1"
                     /translation="GRLSIHQRTVRLLSSRLLLEDLWRRHPEIEDIDLPAPIIVVGLP
                     RSGTTHLVNLIAADSRLRSLPFWESTEPFPLPGEPDDARIERAQQAYELQISMAPLLP
                     AMHHQHPDAVEEEIELEDLDFASYTLEWLARVPRWRDYYFTLDQHHHYAYLRRVLQAL
                     TFRRGPRRWVLKSPQHLEQLGPLVATFPDATFAVTHRDPVSVIASAMTMLAYGDRMRR
                     YAIEPDELSSYWVERVERLLRACVRDHELLPADRTIDVLFHEFMADDVAMVDQIYDRA
                     RLPMTATARRELDAFMGANPRGKHGRLEYDLRGHFGLDPGAVRDRFAFYFERFAVHPE
                     "
     gene            1059..2498
                     /locus_tag="VFX21_14215"
     CDS             1059..2498
                     /locus_tag="VFX21_14215"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEX5097176.1"
                     /translation="MLFFGRRVVVLAAAAALLVAGCSGDDDDSSTTGSSAADAKPAAL
                     TAAASGRPFTEPPRMTAENGVLHFDLVASDRGIDVAGATVQGRAYDDGIVGPTLVVAP
                     GDTIELTLDNQLDAHTNIHFHGLHTSPQNNGDNIFLSVDPGEQFAYSVKIPENHPPGT
                     YWYHSHAHGISEPQVFGGLSGMIIIDGLTARLPADLQGVKDIALAFKDAQVVGTDIEN
                     GPNIDSNAPTLRIVNSQHVPVLAMQPGEVQLWRFANIGADIWYDLALDDQSFVVIGED
                     GNPVWEVWEADHLVLPPGKRYEVLVTGPDGGDTLRTRKYEQGGDTYPATDLMTVESAG
                     DAVATPPMPTALAPVEHIPADEVVEHRTMTFTEQDSENKFYINGKEFDPDRVDVSPKL
                     GTVEEWTLVNKTSEQHPFHIHVNDFEVISIGGEPYAAHGTQDTVALLPGKDVVMRTHF
                     TDFTGKFVFHCHILNHEDNGMMAVVEVVE"
     gene            complement(2507..3262)
                     /locus_tag="VFX21_14220"
     CDS             complement(2507..3262)
                     /locus_tag="VFX21_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5097177.1"
                     /translation="MPVAGVTFDFEGRAVLVTGGTSGIGYAVAVGFAAAGAHVTVTGT
                     KESVASYGVDLSSFAYRRCRMTEPDEISALASSLDRLDVLVNNAGQNLPGGRSEWELD
                     VFEQTVAINLFGAYRLANECRALLEASASPGGAAVVNLASMSSFFGIEVVPGYGAAKA
                     GIVQMTKTLAVAWARHGIRVNAVAPGIVETPMTAPMMDFDEMTKPILARTPAGRFATP
                     DDIAPVVLFLASEAAGYITGQTLPVDGGFSVSG"
     gene            complement(3244..3723)
                     /locus_tag="VFX21_14225"
     CDS             complement(3244..3723)
                     /locus_tag="VFX21_14225"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5097178.1"
                     /translation="MSLPELLARVGPGFFQQAFVVDDLDAARQACETTLGCRNFAVLP
                     AQSLPYRYRGRDVECAIELGFARSGNVQIELLRPTSGEGIHVEFLATNGPGAHHFGFI
                     VDDLDAEVAAASYDEVMAGQFGNLRFVYLDTFAELGAYVELVEDPDRMMMQLCPWRG"
     gene            complement(3710..>6247)
                     /locus_tag="VFX21_14230"
     CDS             complement(3710..>6247)
                     /locus_tag="VFX21_14230"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HEX5097179.1"
                     /translation="ERFVVDHAALRDPAPLSAALHERWAARVPFVVDLQVDADALRAP
                     ETETRAPYRLDPGFEFGREHLYFLARANNYDGRDGRLVWGPDVLAARLGASEGGPADV
                     LLGDGRPAWLDGGPRGTADAPDGHALVHRVRVEAGSLTPEADATTGATLAPDQLAAVT
                     HRGGPARVIAPAGSGKTRVLTERFRLLVAGRGYGAGPVCAVAYNVRAKGEMEQRLVDL
                     GDGPRRKIRTLHALGFDVLRRSRSVREVLSEWDVRRRLEPLVPVRPRANTDVMAPYLE
                     ALSEVRLGLVNPDGVEAKRDDVEGFAAMFEIYRERLHADGAIDHDEQIYGAIEVLCRE
                     PAIRRAFQSECRHLLVDEFQDLTPAQLLMLRLIAAPAYDVFGVGDDDQVIYGYAGADP
                     AFLIDYARYFPGAAHLDLQVNYRCPPAVVRGAANLLTFNRRRVPKEIHAAKTGDDDAL
                     TIERRGAEQLADGAVARVRAVLDAGAPGDDVAVLARVRSVLLPVQLLCLEAGIATNAP
                     VGSDVLERTGVRAALAYLRLAAGWDADALAGGDIAIAARRPTRSIRREIMQRIGGRRR
                     WTRGALRSLANDRTGRLEEFCDDLESLAAALRGGAGTEQLLRLVRDEIGLGRALETLD
                     RSGRGPDASHRDDLHALLALAHLVPDPAGFEPWLRTRLGTPRFDPEPGEVMLSTVHRV
                     KGMEWPHVVVLGAHDGLMPHHLSDDVEEERRVFHVAITRGDTSVHVVAEHGPARFLEE
                     LTRVATPADLAAPVRPVLTVVEPVPMSADEEMIEALKEWRRDRARADAVPAFVILHDS
                     HLMGIAARAPRSLRDLARCPGIGPTKLERYGDEIIATVSSVRPDESS"
BASE COUNT          998 a         2221 c         2120 g          908 t
ORIGIN      
        1 ggccggttgt cgatccacca gcggaccgtc cgcctgctga gcagccgctt gctactcgaa
       61 gacctgtggc gccggcatcc cgagatcgaa gacatcgacc tgcccgcgcc catcatcgtg
      121 gtcggcctcc cgcgctcggg cacgacgcat ctcgtcaacc tcatcgccgc cgactcccgc
      181 ttgcgctcgc tgccgttctg ggagagcacc gaaccgttcc cgctgccggg tgaacccgac
      241 gacgcccgca tcgaacgcgc gcagcaggcc tacgagctgc agatctcgat ggcgccgctg
      301 ctccccgcga tgcaccacca acacccggac gcggtcgagg aggagatcga gctcgaggat
      361 ctcgacttcg cgtcgtacac attggagtgg ctcgcacggg tgccgcgctg gcgcgactac
      421 tacttcacgc tcgaccaaca ccaccactac gcgtacttgc ggcgtgtgct ccaagcgctc
      481 acgttccggc gcggtccgcg ccggtgggtg ctcaagtcgc cacagcacct cgaacagctg
      541 ggtccgctcg tcgcgacgtt ccccgacgcg acgttcgcgg tgacgcaccg cgatccggtg
      601 tcggtgatcg cgtcggcgat gacaatgctc gcgtacggcg atcggatgcg gcgctacgcg
      661 atcgagcccg acgagctgtc gtcgtattgg gtcgagcgcg tcgagcgcct gttgcgcgcg
      721 tgcgtccgcg accacgagtt gctgcccgcc gatcgcacga tcgacgtgct gttccatgag
      781 ttcatggcgg acgacgtcgc gatggtcgac cagatctacg accgcgcccg gctcccgatg
      841 accgccaccg cgcgccgcga gctggacgcg ttcatgggcg cgaatccgcg cggcaagcac
      901 ggacggctcg agtacgacct gcgcggccac ttcggcctcg atcccggagc agtgcgtgac
      961 cgcttcgcgt tctacttcga gcggttcgcc gtccacccgg agtgaaatca cccttcgcgg
     1021 gggtgacagc gcccggtcgg tgcggctagt ccatcgtcat gctcttcttc ggccgacgcg
     1081 tcgtcgtgct ggcggcggcc gccgcgctgc tcgtggcggg gtgcagcggt gacgacgacg
     1141 acagcagcac gaccggcagc agcgccgcgg acgccaagcc cgcggcgctg accgcggccg
     1201 cgtcgggccg acccttcacg gagcctcccc gcatgacggc cgagaacggc gtgctgcact
     1261 tcgatctcgt cgcgagcgac cgcggcatcg atgtcgcggg tgccaccgtg cagggtcggg
     1321 cctacgacga cggcatcgtc ggcccgacac tcgtcgtcgc gccgggcgac acgatcgagc
     1381 tgacgctcga caaccaactc gacgcgcaca ccaacatcca cttccacggg ttgcacacgt
     1441 cgccgcagaa caacggcgac aacatcttct tgtcggtcga ccccggtgag cagttcgcgt
     1501 actcggtgaa gatccccgag aaccacccgc ccggcacgta ctggtaccac tcgcacgcgc
     1561 acggcatctc cgaaccgcaa gtgttcggag ggctgtcggg gatgatcatc atcgacggcc
     1621 tcaccgcgcg gctgccggcc gatctgcaag gcgtcaaaga catcgcgctt gcattcaagg
     1681 acgcacaagt cgtcggtacc gacatcgaga acggtccgaa catcgatagc aacgcgccga
     1741 cgttgcggat cgtgaactcg cagcacgtcc cggtactcgc gatgcagccc ggcgaagtac
     1801 agctgtggcg gttcgcgaac atcggcgccg acatctggta cgacctcgcc ctcgacgacc
     1861 agtcgttcgt cgtgatcggc gaagacggca accccgtgtg ggaggtctgg gaggccgatc
     1921 atctcgtgct gccgcccggc aagcgctacg aagtgctcgt caccggtccg gatggcggcg
     1981 acaccctgcg cacccgcaag tacgagcagg gcggcgacac gtatcccgcg accgatctga
     2041 tgaccgtcga gtcggcgggc gacgcggttg cgacgccgcc gatgccgacg gcgctggcgc
     2101 cggtcgagca catcccggcc gacgaggtcg tcgagcaccg cacgatgacg ttcaccgaac
     2161 aggactcgga gaacaagttc tacatcaacg gcaaggagtt cgatcccgat cgcgtcgacg
     2221 tctcaccgaa gctcggcacc gtggaggagt ggacgctcgt gaacaagacc agcgagcaac
     2281 atccgttcca catccacgtc aacgacttcg aagtcatctc gatcggcggt gagccgtacg
     2341 cggcacacgg cacccaagac accgttgcgc tcctgcccgg caaggacgtc gtgatgcgca
     2401 cgcacttcac cgacttcacc ggcaagttcg tcttccactg ccacatcctc aaccacgagg
     2461 acaacggcat gatggccgtc gtcgaagtag ttgagtaagg agcgggtcag cccgagacgc
     2521 tgaagccgcc gtcgaccgga agcgtctgcc cggtgatgta gcccgcggct tcgctggcga
     2581 ggaacagcac gaccggcgcg atgtcgtcgg gagtcgcgaa ccgtcccgcg ggagtacggg
     2641 cgaggatggg cttcgtcatc tcgtcgaagt ccatcatcgg tgcggtcatc ggtgtctcga
     2701 cgatcccggg cgcgaccgcg ttgacgcgta taccgtggcg cgcccaggca acggcgagcg
     2761 tcttggtcat ctgcacgatg ccggccttcg ccgcgccgta gccggggacg acctcgatac
     2821 cgaagaacga gctcatcgac gcgaggttca cgaccgcggc gccacccggc gacgcgctgg
     2881 cctcgagcaa cgcccggcac tcgttcgcca atcggtacgc accgaagagg ttgatggcga
     2941 cggtctgctc gaacacgtcg agctcccact cgctgcgacc accgggcagg ttctggcccg
     3001 cgttgttgac gagcacgtcg agacggtcca gcgaggacgc gagcgcactg atctcgtcgg
     3061 gctcggtcat gcggcagcgc cggtaggcga acgacgacag gtcgacgccg tagctcgcga
     3121 ccgactcctt cgtgccggtc actgtcacgt gcgcgcccgc tgccgcgaag cccacggcga
     3181 cggcgtagcc gatgcccgat gtgccgccgg tgacgagcac ggcgcgtcct tcgaagtcga
     3241 acgtcacccc cgccacgggc acaactgcat catcatgcgg tcggggtctt cgaccagctc
     3301 gacgtaggcg ccgagctcgg cgaacgtgtc gaggtagacg aagcgcaggt tcccgaactg
     3361 accggccatc acctcgtcgt acgacgcggc ggcgacttcg gcgtcgaggt cgtcgacgat
     3421 gaagccgaaa tggtgcgcgc ccgggccgtt cgtcgcgagg aactcgacgt ggatgccttc
     3481 tccgctcgtc gggcgcagca actcgatctg cacgttgccg ctgcgggcga agccgagctc
     3541 gatcgcgcac tcgacgtcgc gaccgcggta gcggtacggc aacgattgcg cggggagcac
     3601 cgcgaagttc ctgcacccga gcgtcgtctc gcaggcctgg cgggcggcgt cgaggtcgtc
     3661 gaccacgaac gcctgctgga agaacccggg accgacgcgt gccagcagct caggaagact
     3721 catccgggcg gacactactg accgtcgcga tgatctcgtc gccgtagcgc tccagcttcg
     3781 tcggcccgat cccggggcag cgggccaggt ctcgcagcga gcgaggggcg cgggcggcga
     3841 ttcccatgag gtgcgagtcg tggaggatca cgaacgcggg aacggcgtcg gcgcgcgccc
     3901 gatcgcgccg ccactccttc agcgcctcga tcatctcctc gtcggcggac atgggcacgg
     3961 gctcgacgac cgtgagcacc gggcgtaccg gcgcggcgag atcggccggg gtcgcgacgc
     4021 gggtcagctc ctccaggaac cgcgccggac cgtgctcggc gacgacgtgc accgacgtgt
     4081 caccgcgggt gatcgcgacg tggaacacgc gccgctcctc ttcgacgtcg tcggagaggt
     4141 ggtgcggcat gaggccgtcg tgcgcgccga gcacgacgac gtgcggccat tccatgccct
     4201 tgacgcggtg caccgtcgac agcatcacct cgcccggctc cggatcgaag cgcggcgtac
     4261 cgagccgggt gcgcaaccag ggctcgaagc cggcggggtc gggcacgagg tgcgcgagcg
     4321 cgagcagcgc gtgcaggtcg tcgcggtggc tggcgtcggg accacgcccc gaccgatcga
     4381 gcgtctccag cgcccggccg agcccgatct cgtcgcgcac caatcgcaac agttgctcgg
     4441 taccggcacc gccccgcagc gccgccgcga gcgattcgag atcgtcgcag aactcttcga
     4501 ggcgcccggt cctgtcgttg gccaacgatc gcagtgcgcc gcgcgtccag cggcggcggc
     4561 cgccgatgcg ctgcatgatc tcgcgccgga tcgatcgggt cgggcggcgc gccgcgatgg
     4621 cgatgtcgcc gcccgcgagt gcatcggcat cccagcccgc ggccaaccgt aggtatgcga
     4681 gcgcggcgcg cacgccggtg cgttcgagca cgtcgctgcc caccggagcg ttggtcgcaa
     4741 tgcccgcttc gaggcacagg agctggaccg gcaggagcac ggagcggacc cgcgccagca
     4801 ccgcgacgtc gtcgcccggc gcccccgcgt cgagcaccgc gcgtacgcgc gcgaccgcgc
     4861 cgtcggccag ctgctcggca ccgcgccgct cgatcgtgag cgcgtcgtcg tcgccggtct
     4921 tggctgcgtg gatctccttc gggacccggc gccggttgaa cgtcagcagg ttcgcggcac
     4981 cccgcaccac ggccggcggg cagcggtagt tcacctgcag atcgaggtgc gccgcgcctg
     5041 ggaagtagcg cgcgtagtcg atgaggaacg cgggatcggc gcccgcgtac ccgtagatca
     5101 cctggtcgtc gtcgcccaca ccgaacacgt cgtacgcggg cgcggcgatc aaccgcagca
     5161 tcagcagttg cgcgggcgtg aggtcttgga actcgtcgac cagcagatgg cgacactcgg
     5221 actggaacgc gcgccggatc gccggttcgc ggcacaacac ttcgatcgcg ccgtagatct
     5281 gctcgtcgtg gtcgatggcg ccgtcggcgt gcagccgctc gcgatagatc tcgaacatcg
     5341 ccgcgaagcc ctcgacgtcg tcgcgcttcg cttcgacacc atcggggttc acgagcccga
     5401 gccgcacctc cgacaacgct tcgagatacg gcgccatgac atcggtgttg gcgcgcgggc
     5461 gcaccggcac cagcggttcg agccgccggc ggacgtccca ctcgctcagg acctcccgca
     5521 cactgcgcga ccgccgtagc acgtcgaacc cgagcgcgtg caacgtgcgg atcttgcggc
     5581 ggggcccgtc gccgaggtcg accagccgct gctccatttc gcccttggcg cggacgttgt
     5641 acgcgaccgc gcagaccggg cccgcgccgt agccgcgccc ggcgaccagc aaccggaagc
     5701 gttcggtgag cacgcgcgtc ttgcccgagc ccgcgggtgc gatcacgcgc gcgggaccgc
     5761 cgcggtgcgt caccgccgcg agctggtcgg gtgcgagcgt cgccccggtc gtcgcatcgg
     5821 cctcgggcgt gagcgatccc gcttcgacgc gtacgcgatg gaccagcgca tgaccgtcgg
     5881 gtgcgtcggc ggtgccgcgc ggtccgccgt cgagccacgc ggggcggccg tcgcccaaca
     5941 acacgtcggc cgggccgccc tcgctcgcac cgagccgcgc cgccaagaca tcgggccccc
     6001 agacgaggcg gccgtcgcgc ccgtcgtagt tgttggcacg tgcgaggaaa tagaggtgct
     6061 cgcgcccgaa ctcgaagccg gggtcgaggc ggtacggcgc gcgtgtctcg gtctcgggcg
     6121 cccgtaacgc gtcggcgtcg acctggaggt cgacgacgaa cggcactcgc gctgcccacc
     6181 gctcgtgcag cgcggcgctc agcggcgccg ggtcgcgcag cgcggcgtgg tcgactacga
     6241 accgctc
//
LOCUS       DASUGA010000718         2688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1759333, whole genome shotgun sequence.
ACCESSION   DASUGA010000718 DASUGA010000000
VERSION     DASUGA010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759333"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..1317)
                     /locus_tag="VFX21_14235"
     CDS             complement(241..1317)
                     /locus_tag="VFX21_14235"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5097180.1"
                     /translation="MRIGVIGVKGIGQAHLWALHGVDGSDLVAVCDVDAAAAEKNGAD
                     HNAAVFTDAEALFASGDVDAVVIATPPGTHAPLVRSALAAGMHVYCEKPFTPTADEGY
                     ELARYAKAVERTLQVGFQFRFHLGYAAMKNAVASIAPVRRVNVTATNWFRADKYFTAS
                     PWRGTWRVAGGGVLMAQAVHQVDALINAVGMPASVTGRVRAALHDVDAGLPLVNPGTM
                     QTFVKQSMADRDLTMTLLVVFASLALILASLGVYSVMAYSVTQRSGEIGIRMALGARP
                     LDVQKMVIRQGMKLAALGLMIGAGLALTLTRLLNSLLFEVSAYDPLTYVTITLILCAV
                     AVSACWIPSRRASRIDPTQALHEA"
     gene            complement(1382..>2688)
                     /locus_tag="VFX21_14240"
     CDS             complement(1382..>2688)
                     /locus_tag="VFX21_14240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX5097181.1"
                     /translation="LLGHVNLEAGLNTPASARERGAPTLDRIGGLLAYFGSPELEYPA
                     VQITGTNGKTTTAHVASTLLTASGLRVGTITSPHLERVNERMEVDGEPVDDDTLDELL
                     RAAQLVEDAVGIEASYFEILVAIALRWFADEAVDVAVVEVGMGGTWDATNLVDGQVGV
                     VTNVAVDHVEYLGPTREAIATEKAGIVKPGSTLVLGETDPKLRPIFEAREADRVVVRD
                     EQFGVRRNVLAIGGRLVDLYTARAQYPDVYLPLHGAHQADNAAIALQAAESFVDAELP
                     HEIVELAFRAVRSPGRLEVVGRQPLVLLDGAHNVAGAHALRTALAEEFGDDTARTLVV
                     GLLREKEPHEMLSALGLDDLAKDERGGVLVCCRPPSPRALLPQFVAQAAIEMGFSAER
                     IEVVDTVPQAVSAALLATSEDGQIVITGSLYVVGAARGVLVK"
BASE COUNT          432 a          957 c          874 g          425 t
ORIGIN      
        1 atgtggatgc acctgagccg cggcgatacg ctgccgggat cgcgccgctt cgaaacgcgt
       61 ccgcgcggcg cgcaggttct cgatcgtacg gcgcttggac tcggcgccgg cgagtgacgg
      121 cgcgcgtcac gccgatccgc aagcccgcgc gcgttcgcgt ttgcgaccgt tccacgcgca
      181 cgcagcgtgc gtacgccgcg gatgcaatcc cgaagcaccc gcgtgcccca agccgctgcg
      241 ctacgcctcg tgcaacgcct gcgtcggatc gatgcgcgac gccctgcgcg atggaatcca
      301 gcaggccgac accgcgaccg cgcacaggat cagcgtgatc gtcacgtagg tcagcggatc
      361 gtacgcgctg acttcgaaca gcaggctgtt cagcagtcgc gtcagcgtca gcgccagacc
      421 cgcgccgatc atcaggccca gcgcagccag cttcatgcct tgccggatga ccatcttctg
      481 cacgtcgagc ggccgcgcgc cgagcgccat gcgtattccg atttcgccgg accgctgcgt
      541 caccgaatag gccatcacgc tatagacgcc aaggctcgcc agaatcagtg caaggctcgc
      601 gaacacgacg agcagcgtca tggtcaggtc gcggtcggcc atcgactgct tgacgaacgt
      661 ctgcatcgtg cccggattga ccagcggaag gccggcgtcg acgtcgtgca gcgcggcgcg
      721 cacgcggccg gtcacgcttg cgggcatccc gaccgcgttg atcagcgcgt cgacctggtg
      781 cacggcttgc gccatcaaca caccgccgcc ggccactcgc catgtgccgc gccacggact
      841 ggcagtgaag tacttgtcgg cgcggaacca gttcgtcgcg gtgacgttca cgcgccgcac
      901 cggcgcgatc gacgcgaccg cgtttttcat cgccgcgtat ccgaggtgga agcggaactg
      961 gaacccgacc tgcagcgttc gctcgaccgc ctttgcgtag cgggcgagtt cgtagccttc
     1021 gtcggccgtg ggcgtgaacg gcttctcgca atacacgtgc attccggccg caagcgcact
     1081 gcgtacgagc ggcgcgtgcg tgcccggcgg cgtcgcgatc acgaccgcgt cgacgtcccc
     1141 gctcgcgaac agtgcctcgg cgtcggtgaa caccgcggcg ttgtgatcgg cgccgttctt
     1201 ctcggccgcg gcggcgtcga cgtcgcacac cgcgacgagg tccgacccgt cgacgccgtg
     1261 cagcgcccac aggtgcgcct gcccgatgcc cttaactccg atgacgccga tcctcaccgc
     1321 tcctcctccc gaacttgcgt gagaaacggc cctctccggg gcactttctc acgcatgttc
     1381 gtcatttgac caggacgcca cgagccgcac cgacgacata cagcgaaccg gtaatcacga
     1441 tttggccgtc ttcggaggtc gcgagcagcg cggcgctgac cgcttgggga accgtgtcga
     1501 cgacctcgat gcgctcggcc gaaaagccca tctcgatcgc ggcctgggcg acgaactgcg
     1561 gcagcaaggc acgcgggctc ggcgggcggc aacagacgag cacgccgccg cgttcatcct
     1621 tggcgaggtc gtcgaggccg agcgcgctca gcatctcgtg cggttccttc tcacgcagca
     1681 acccaacgac cagtgtgcgc gccgtgtcgt cgccgaactc ctcggccagc gcggtccgca
     1741 gcgcatgagc gccggccacg ttgtgcgcgc cgtcgagcaa caccagcggc tgccggccga
     1801 cgacttcgag ccggcccggt gagcgcaccg cccggaacgc cagctcgaca atctcatgcg
     1861 gtagctcggc gtcgacaaac gactcggccg cttgcagcgc gatcgcagcg ttgtcggctt
     1921 ggtgcgcgcc gtgcagcggc agatacacgt ccggatactg ggcgcgcgcc gtgtagaggt
     1981 cgaccaaccg gccgccgatt gccagcacgt tgcggcgcac gccgaactgc tcgtcgcgca
     2041 cgaccacgcg gtcggcctcc cgagcttcga aaatcggccg caacttggga tcggtttcgc
     2101 cgaggacaag cgtggaaccc ggcttcacga tgccggcctt ctcggtggcg atcgcctcgc
     2161 gcgtcgggcc gaggtattcg acgtggtcga ccgcgacgtt cgtcacgaca cccacttggc
     2221 cgtcgacaag gttggtcgcg tcccacgtgc cgcccatgcc gacctcgacc actgcaacgt
     2281 cgaccgcttc gtcggcgaac caccgcaacg cgatcgcgac gaggatctcg aaataggacg
     2341 cctcgatgcc gaccgcgtct tcaaccagct gggccgcgcg cagcagttcg tcgagcgtgt
     2401 cgtcgtcgac gggctcgccg tcgacctcca tccgctcgtt gacccgttcg aggtgcgggc
     2461 tcgtgatcgt gcccacccgc aaaccagatg cggtcagcaa cgtcgaggcg acgtgtgcgg
     2521 tcgtcgtctt gccgttggta ccggtgatct gcacggccgg gtactcgagc tcgggcgacc
     2581 cgaagtaggc gagcaacccg ccgatgcgat cgagcgtggg ggcgccgcgt tcacgcgccg
     2641 acgcaggcgt gttcagtccc gcttcgagat tgacgtgacc gaggagcc
//
LOCUS       DASUGA010000719         2963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_352424, whole genome shotgun sequence.
ACCESSION   DASUGA010000719 DASUGA010000000
VERSION     DASUGA010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352424"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VFX21_14245"
     CDS             complement(<1..759)
                     /locus_tag="VFX21_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013288415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5097182.1"
                     /translation="MPRTSFDSHGRRRRALVAGAAIALAASTLGAGVAGAQGKSSQPV
                     NVRLGYFPNVTHAPALVGVESGLFQKALGKNTLDVKTFNAGPEEVTALLAGALDIGYI
                     GPNPSVNAYVQSNGEAVRVVGGSTSGGAYLVVKPEITKATDLKGKRVATPQLGNTQDV
                     ALRTWLKKAGLKTDAQGGGDVSIAPQENALTLDAFKQGQIAGAWVPEPWATRLVTEAG
                     GKVLVDERTLWPEGKYVTTNIIVRTQFLDEHPDVV"
     gene            885..1373
                     /locus_tag="VFX21_14250"
     CDS             885..1373
                     /locus_tag="VFX21_14250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX5097183.1"
                     /translation="MWISRRTDYATRALVALALEEDVRPVKIQELAERTDTPISILEQ
                     VLPQLRSAGIVRSERGPSGGYRLNHAPADITLERVVRVLQGPLAPIACATRHEPEPCT
                     MLAGCALHETWAEVRDATITILERTTFADLAARSSGRWVAGEVEVGAPTRRSTRATTA
                     AE"
     gene            1389..2516
                     /locus_tag="VFX21_14255"
     CDS             1389..2516
                     /locus_tag="VFX21_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5097184.1"
                     /translation="MPIHLHWFLPTGGDSRDVLATGPDVHRRDPTLDHLALVARTAEQ
                     LGFEAVLTPTGTWCEESWISTAALIRETTRLKFLVAFRPGTITPTLAAQMAATFQRHS
                     NGRALLNVVTGGDAGEQQRFGDWLDHDARYERTDEFLAILRGAWSGDPYDFDGAHYHV
                     AGASTRLPPDPAPDIYFGGASETAERVAARRADVYLTWGEPPDLVAPRVERVRKLAAD
                     EGRAVRFGIRLHVISRDRSADAWAETERLLESMDDATIARVQQDLAKSQSVGQARMQG
                     LHGGRRDELVVAPNLWAGIGLVRGGAGTALVGDHDEIAARIAEYHELGFTEFILSGHP
                     HLEEAFWVGEHVRPRLQRMGLVAPDPSPIASGEHRAVSAAR"
     gene            complement(2538..>2963)
                     /locus_tag="VFX21_14260"
     CDS             complement(2538..>2963)
                     /locus_tag="VFX21_14260"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5097185.1"
                     /translation="VTALAYTVGAAESALDLSVQHARDRQQFGTPIGSFQAVAHRCAD
                     MRAEIDACRLLAYRAAWALGGDHEHVDVAFAVSSALGYAKDALRRVAMHAHQVHGAIG
                     FSTEHDLHLFTRRVKAFELTAGSTARHQERFASSIGLRA"
BASE COUNT          438 a         1033 c         1037 g          455 t
ORIGIN      
        1 cacgacgtcg gggtgctcgt cgaggaactg cgtccgcacg atgatgttcg tcgtgacgta
       61 cttgccctcg ggccagagcg tgcgctcgtc gacgaggacc ttgccgcccg cctcggtgac
      121 gaggcgcgtc gcccacggct cgggtaccca cgcgcccgcg atctggccct gcttgaacgc
      181 gtcgagtgtg agcgcgttct cctgcggtgc gatcgacacg tcgccgccgc cctgcgcgtc
      241 agtcttcagc cccgccttct tcaaccaggt gcgcagcgcg acgtcctggg tgttgccgag
      301 ctgcggggtc gccacgcgct tgcccttgag gtcggtcgcc ttggtgatct cgggcttcac
      361 cacgaggtag gcgccgcccg acgtcgagcc gccgaccacg cgcacggcct cgccgttcga
      421 ctgcacgtag gcgttcacac tcgggttcgg gccgatgtag ccgatgtcga gggcgcccgc
      481 gagcagcgca gtgacctctt ccggtccggc gttgaacgtc ttcacgtcga gcgtgttctt
      541 gcccagcgcc ttctggaaca gcccgctctc gacgccgacg agcgccggcg cgtgggtgac
      601 gttggggaag tacccgaggc ggacgttcac cggttgcgac gacttgcctt gcgcgccggc
      661 gacacccgcg cccagcgtgc tggcggcaag cgcgatggcg gcgccggcga cgagcgcgcg
      721 gcgccggcga ccgtgggagt cgaacgaggt acggggcatg ggggaggcct ttgctggtga
      781 gataattccg acttgaatag tcgggtatat aagtatcccg ccgggtccgg gtcaagtcgc
      841 ttcggaaagt ttcttggtaa gtcgactgat agagtcgaca ttcgatgtgg atctcccggc
      901 ggaccgacta cgccacccgc gcgctcgtcg ccctggccct cgaagaagac gtccggccgg
      961 tgaagatcca ggagctggcc gagcgcaccg acacgccgat ctcgatcctc gagcaggtgt
     1021 tgccccagct gcggagcgcc gggatcgtcc gttcggagcg cgggccgagc ggtggatacc
     1081 gcctgaacca cgcgccggcc gacatcacgc tcgagcgcgt ggtccgggtg ctccagggcc
     1141 cgctcgcgcc gatcgcgtgc gcgacccgtc acgagcccga gccctgcacc atgctcgccg
     1201 gctgcgcgct ccacgagacg tgggccgaag tacgcgacgc gacgatcacc atcctcgagc
     1261 gcacgacctt cgccgacctc gcggctcgct cgtcgggacg gtgggtcgcc ggcgaggtcg
     1321 aggttggcgc accgacccgc cgatcgacgc gagcgaccac cgccgccgag tgaccactcc
     1381 acgtcgccgt gccgatccac ctccactggt tcctgccgac gggcggcgac agccgcgacg
     1441 tgctcgcgac gggtcccgac gtccatcgtc gtgatccgac gctcgaccac ctcgcgctcg
     1501 ttgcccgaac tgccgagcag ctcggcttcg aagcggtgct caccccgacc gggacgtggt
     1561 gcgaggagtc gtggatctcc acggcggcgc tcatccgcga gacgacgcgg ctgaagttcc
     1621 tcgtcgcgtt ccggccggga acgatcacac cgacgctcgc cgcgcagatg gcggcgacgt
     1681 tccagcgcca ctccaatggt cgtgcgttgc tcaatgtcgt gacgggcggc gatgccggag
     1741 aacaacaacg cttcggcgac tggctcgacc acgatgcgcg atatgagcgc accgacgaat
     1801 tcctcgcgat cttgcgcggc gcgtggagcg gcgacccgta cgacttcgac ggcgcgcact
     1861 atcacgtcgc cggtgcgtcg actcgcctgc caccggaccc cgcacccgac atctacttcg
     1921 gcggtgcatc ggagacggcc gaacgcgtgg ctgcgcgccg agccgacgtc tacctcacat
     1981 ggggcgaacc gccagatctc gtcgcgccgc gtgtggagcg agtgcgcaag ctcgcggccg
     2041 acgaaggccg cgccgtgcgg ttcgggatcc ggctccacgt gatcagccgg gaccggtcgg
     2101 cggacgcgtg ggccgagacc gagcgcctgc tcgagtcgat ggacgacgcg acgatcgcgc
     2161 gggtgcaaca ggatctcgcg aagagccagt cggtcggcca ggcacgaatg cagggcctgc
     2221 acggcggtcg gcgtgacgag ctcgtcgtcg cgccgaacct gtgggccgga atcggtctcg
     2281 tgcgcggggg cgcgggcacc gcactcgtcg gcgaccacga cgagatcgcg gctcgtatcg
     2341 ccgagtacca cgagctcggc ttcaccgagt tcatcctctc gggccacccg cacctggagg
     2401 aagcgttctg ggtcggcgag cacgtgcggc cgcggcttca gcgcatgggc ctcgtggcac
     2461 ccgatccgtc gcccatcgcg agtggtgagc accgtgcggt gagcgcggcg cgctgaccgc
     2521 gcggaaccgc gcgaccctca ggcgcgcagg ccgatcgacg acgcgaagcg ttcctggtga
     2581 cgcgcggtgc tccccgcggt gagctcgaac gccttgacgc gccgcgtgaa gaggtgcaga
     2641 tcgtgctcgg tggagaaccc gatcgcaccg tgcacttggt gggcatgcat cgccactcgc
     2701 cggagtgcgt ccttcgcata gccgagcgcg ctgctgaccg cgaacgcgac gtcgacgtgc
     2761 tcgtggtcac caccgagtgc ccacgcggcg cggtaggcga ggaggcgaca cgcgtcgatc
     2821 tccgcgcgca tgtcggcgca acggtgggcg acggcctgga acgatccgat cggcgtgccg
     2881 aactgctgcc gatcgcgtgc gtgctgcacc gagagatcga gcgcgctctc tgcggcgccc
     2941 acggtgtagg ccagtgcggt cac
//
LOCUS       DASUGA010000720         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_804571, whole genome shotgun sequence.
ACCESSION   DASUGA010000720 DASUGA010000000
VERSION     DASUGA010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804571"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="VFX21_14265"
     CDS             complement(<1..1488)
                     /locus_tag="VFX21_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX5097186.1"
                     /translation="MGFWKYAQDDPGYLAIVDPDGTEHLSGDVLARANQVVHALRALG
                     LEKGDTFAAVLPNGANPFHLYLAALQAGWYYVPINYRLSPSEIAYIITDSDAKAFISH
                     ERFADIVSKAADEAKVPQNARLAHGTIPGFRSFGEYVDEFPTDLPENRATGASMHYTS
                     GTTGKPKGVKRALNDLDPDTSAELFTFLLGLFNIPAGPGEVHLSTSPNYHTAVTTFAG
                     NSLHMGHTVVCMDKWDAEETLRLIQTYKCSHTHMVPTQFIRMLQLPDDVKAKYDVSAM
                     KWAIHAAAPCPVDTKRKMLEWWGPVIWEYYAATEGGGTIAPPDEWIKYPGTVGRAWPT
                     SELKITDDDGNVMPTGEPGTVWMRMGTANFEYKGDKEKTKKTFDAEGYFTVGDVGYFN
                     DDGYLFLCDRKSDMIIAGGINIYPAEIESCIHEVPGVADVAVFGIPHVDLGEQILAVV
                     EPLPGVAPDDALREQVMAHVREKLGKYKWPRQIDFMDELPREPTGK"
     gene            complement(1540..>2004)
                     /locus_tag="VFX21_14270"
     CDS             complement(1540..>2004)
                     /locus_tag="VFX21_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097187.1"
                     /translation="HRGSDTMTAVTTRPAAAEFTTLDRPAYHHPSKSGLVSIVVASGL
                     IAFWAAVILVTISVPAGLVGIAIAIGAGLCDAAFNTPTAIDVDNGGVTLHYWRRTKRL
                     AATEVSVTHIVPRDKFIVARRGKRRRVMQFRENRPQRAVRAFMAASIELISR"
BASE COUNT          316 a          648 c          661 g          379 t
ORIGIN      
        1 tttgccggtg ggttcgcgcg gcagttcgtc catgaagtcg atctggcgcg gccacttgta
       61 cttgccgagc ttctctcgta cgtgcgccat gacctgctcg cgcaacgcgt cgtcgggtgc
      121 gacgcccggg agcggctcga cgactgcgag gatctgctcg ccgaggtcaa cgtgtggaat
      181 gccgaacact gcgacgtcgg cgacgcccgg cacttcgtga atgcagctct cgatctcggc
      241 cgggtagatg ttgataccgc ccgcgatgat catgtcgctc ttgcggtcgc acaagaagag
      301 gtagccgtcg tcgttgaagt acccgacatc gccgacggtg aagtagccct cggcgtcgaa
      361 ggtcttcttc gtcttctcct tgtcgccctt gtattcgaag ttggcggtac ccatgcgcat
      421 ccacacggtg ccgggctcgc cggtcggcat cacgttgccg tcgtcgtcgg tgatcttcag
      481 ctcgctggtc ggccacgcgc gcccgacggt gccggggtac ttgatccact cgtcgggcgg
      541 cgcgatcgtg ccgccgcctt cggtcgcggc gtagtactcc cagatcaccg ggccccacca
      601 ctcgagcatc ttgcgcttgg tgtcgaccgg gcagggcgcg gccgcgtgga tcgcccactt
      661 catcgccgag acgtcgtact tcgccttcac gtcgtcggga agctgcaaca tgcggatgaa
      721 ctgcgtcggc accatgtgcg tgtgcgagca cttgtacgtc tggatgaggc gcagcgtttc
      781 ttcggcgtcc cacttgtcca tgcacacgac ggtgtggccc atgtgcagcg agttgcccgc
      841 gaacgtcgtg accgcggtgt ggtagttcgg cgacgtcgac aggtgcactt cacccggacc
      901 ggccggaatg ttgaagagcc cgaggaggaa cgtgaacagc tcggcactcg tgtcggggtc
      961 gaggtcgttc agcgcgcgct tcacgccctt gggcttcccg gtcgtccccg acgtgtagtg
     1021 catcgacgcg ccggtcgccc ggttctcggg caagtcggtc gggaactcgt cgacgtactc
     1081 gccgaaggag cggaagccgg ggatcgtgcc gtgcgcgagc cgcgcgttct gcggcacctt
     1141 cgcctcgtcg gcagccttgc tcacgatgtc ggcgaaccgc tcgtggctga tgaaggcctt
     1201 cgcgtcggag tcggtgatga tgtacgcgat ctccgacggg ctcagccggt agttgatcgg
     1261 cacgtagtac caaccggcct ggagcgcggc gaggtacagg tggaacgggt tggcgccgtt
     1321 cggcaggact gcagcaaacg tgtcgccctt ctcgagcccg agcgcacgca gcgcgtgcac
     1381 gacttgattg gctcgcgcca gcacgtcacc actgaggtgc tccgtgccgt cagggtcgac
     1441 aatcgcgaga taacccggat cgtcctgcgc gtatttccaa aagcccatga cgtgtcctcc
     1501 cccaggccgt gtgcggccgc agagtagaac acgttctcgt tagcgggaaa tcagctcgat
     1561 gctggcagcc atgaacgcgc ggactgctcg ttgcgggcgg ttctcacgga actgcatcac
     1621 gcggcggcgc ttgccccgac gcgccacgat gaacttgtcg cgcggcacga tgtgcgtcac
     1681 cgagacctcg gtagcagcaa ggcgcttcgt ccgccgccag tagtgcaacg tgacgccgcc
     1741 attgtcaacg tcgatcgccg tcggcgtgtt gaacgcggcg tcgcacaagc ccgccccgat
     1801 ggcgatcgcg atgccgacca gcccggccgg aaccgagatc gtcacgagga tcacggccgc
     1861 ccagaaggcg atgagcccac tcgcgaccac aatcgagacc aaccccgact tcgaagggtg
     1921 gtggtatgcc gggcggtcga gcgtggtgaa ttcagccgcc gccggccgag tcgttactgc
     1981 agtcatggtg tcactccccc tgtg
//
LOCUS       DASUGA010000721         3327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_406161, whole genome shotgun sequence.
ACCESSION   DASUGA010000721 DASUGA010000000
VERSION     DASUGA010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406161"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VFX21_14275"
     CDS             complement(<1..632)
                     /locus_tag="VFX21_14275"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX5097188.1"
                     /translation="MPLFTRDDRAATGASALVFSLIAVVFAFGALLTAIYADNDDAKA
                     ASAAGGTSVSLTEFAITPNSATVPIGGELVVTNSGTATHNLGIDGTDLKTPDLAPGES
                     ATLDLSKLEAGMYTMYCEIAGHKGAGMVGDLMVGDSTGHAEHAVNQDRLLAENDASDA
                     LMKEPVDAYVKQLTEGANTEGVGNQKLEPNVLLDGTKVFDLTADLVEWQV"
     gene            740..1381
                     /locus_tag="VFX21_14280"
     CDS             740..1381
                     /locus_tag="VFX21_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097189.1"
                     /translation="MSETGTKLRVLVVEPQAHDADQAVEELLAAGHEVARCVEAGQPS
                     FPCAAYREGCPLECGRPIDVVVDVRRHARSQLAPGEFGMSCAVRKHVPLVLVGPTILH
                     PFGDFAVESTTDGRDVVRACERAVARPLPAHSRAATNALTEYLDGHAPDVTRAKVEVY
                     RRNGALAVTVSCEPALPVSMRSMAGTRIVAAVRAIDHHASGIDVVFADEVLVS"
     gene            complement(1330..1869)
                     /locus_tag="VFX21_14285"
     CDS             complement(1330..1869)
                     /locus_tag="VFX21_14285"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5097190.1"
                     /translation="MTDAILQRTGDRPVLRFERRFARPIDDVWRAVTDPEEMRSWFPT
                     RIDIDEWRVGVEFTHVFEDHDAPPLPGVVLEWDPPRRVSFTWGTDTITFELEADGDGT
                     KFVLTEELGANIAARNAAGWEECLALLESGSTSVDWQTRFDHYVDVFQPVLGPQDGPP
                     PEFQDTSTSSANTTSMPLA"
     gene            complement(1866..2204)
                     /locus_tag="VFX21_14290"
     CDS             complement(1866..2204)
                     /locus_tag="VFX21_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5097191.1"
                     /translation="MAVSTTLEILATPTRRELLDALREGEQPVSELVRRVALSQPAVS
                     KQLRILRDAGLVTVRADGQRRIYRLRSEPLAELFDWLTPYRELWESSLDRLAQHLTPQ
                     SPADDEESPR"
     gene            2277..>3327
                     /locus_tag="VFX21_14295"
     CDS             2277..>3327
                     /locus_tag="VFX21_14295"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX5097192.1"
                     /translation="MRILYLDVDTLRADHVGCYGYHRATTPNIDALAADGVRFANVYA
                     SDVPCLPSRTALITGAFGIRNGVVNHGGAAADLRSLGAEREFVSRWAWHSWAGLFYNA
                     GWHTASLSSFPFRHSASWWNAGFMEAMNLMRGMGGERADHVLPGALDWLDRRGRNDRW
                     FLHVHLWDPHTPYNTPADYGNPFASDPVSAWHTDAVRAHNWSLSGPHSAQEPWGFRPD
                     EWGDPPPREPWDASSMDAVKQIFDGYDVGIRYADDALGTLCNKLADIGVLDDTAILVS
                     TDHGEAFGELGVYADHQAADEATCHIPAVLRWPGLAPNVHDGLQYHLDVAATLVDLAG
                     IKVPRRWDGVPIDIGG"
BASE COUNT          498 a         1116 c         1170 g          543 t
ORIGIN      
        1 tcgacctgcc actcgacgag gtcggcggtg aggtcgaaca ccttcgtacc gtcgagcagg
       61 acgttcggct cgagcttctg gttgccgaca ccttcggtgt tcgcgccctc ggtcagctgc
      121 ttgacgtacg cgtcgacggg ctccttcatc aacgcgtcgg aagcgtcgtt ctccgcgagc
      181 agccggtcct ggttgaccgc gtgctccgcg tggcccgtgc tgtcgccgac catgaggtca
      241 ccgaccatgc ccgcaccctt gtgccccgcg atctcgcagt acatcgtgta catgcccgct
      301 tcgagcttgc tcaggtcgag ggtcgccgac tcaccgggtg cgagatcggg cgtcttgagg
      361 tcggtgccgt cgataccgag gttgtgggtc gcggtcccgc tgttggtgac gacgagctcg
      421 ccgccgatcg gcaccgtcgc ggagttcggc gtgatcgcga actcggtgag cgacaccgag
      481 gtgccgccgg ccgccgatgc cgccttggcg tcgtcgttgt ccgcgtatat cgcggtgagc
      541 aacgcgccaa acgcgaacac gacggcgatg agcgagaaca caagggcact ggcgccggtc
      601 gcggcgcggt cgtcgcgggt gaagagaggc atgacgtcat ccaagcgatc tcgcgggcgc
      661 ccgggcaggg ccgaatcgtt ctcgtgactc gggactttcg gccctatagg cggcgcggcc
      721 ggaggctcag gctgcacgta tgagcgaaac cggaacgaag cttcgggtcc tggtcgtcga
      781 gccccaggcc cacgatgccg atcaggccgt cgaggagctc ctggcggccg ggcacgaggt
      841 cgcccggtgc gtcgaggcgg gacaaccgag ctttccgtgc gcggcctacc gggaagggtg
      901 tccgctcgaa tgcggccgtc cgatcgacgt cgtggtcgac gtccgccggc acgcccgctc
      961 gcagctggcg ccgggcgagt tcggcatgag ctgcgcggtc cgcaagcacg tgccgctcgt
     1021 gctcgtcggg ccgacgatcc tgcacccctt cggcgacttc gcggtcgagt cgaccaccga
     1081 cgggcgtgat gtggtccggg cgtgcgagcg cgccgtggcc cggcccctcc ctgcgcacag
     1141 tcgcgccgcg acgaacgcgt tgaccgagta cctggatggt cacgcgcccg acgtgacgag
     1201 ggcgaaggtc gaggtgtacc ggcgcaacgg cgcgctcgcc gtgacggtga gttgcgagcc
     1261 cgcgctaccg gtgtcgatgc gctcgatggc cggcacccga atcgtcgcgg cggtgcgcgc
     1321 aattgatcat cacgcgagcg gcatcgacgt cgtgttcgcc gacgaggtac tggtgtcctg
     1381 aaactccggc ggcgggccgt cttggggacc cagcacgggc tggaagacgt cgacgtagtg
     1441 gtcgaaccgc gtctgccagt cgacagacgt cgagccggat tcgagaagcg cgagacattc
     1501 ctcccaaccg gccgcgttgc gcgccgcgat gttggcgccg agctcttcgg tgagcacgaa
     1561 cttcgtgccg tcgccgtcgg cctccaactc gaacgtgatg gtgtcggtgc cccacgtgaa
     1621 cgagacgcgg cgcggcggat cccactcgag caccacgccc gggagcggag gcgcgtcgtg
     1681 gtcttcgaac acgtgcgtga actccacgcc gacgcgccac tcgtcgatgt cgatgcgcgt
     1741 cgggaaccat gaccgcatct cttcgggatc ggtgacggcg cgccacacgt cgtcaatcgg
     1801 acgggcgaaa cggcgttcga agcggaggac gggccggtcg cccgtacgtt gcaggattgc
     1861 gtcggtcatc ggggggactc ctcgtcgtcg gcgggcgact gcggtgtgag gtgttgtgcg
     1921 agccggtcga gactcgactc ccacagctcc cggtacgggg tgagccagtc gaacagctcg
     1981 gcgagtggct cggaacgcag gcggtagatc cgccgctggc cgtccgcgcg cacggtcacg
     2041 aggcccgcgt cgcgcaggat gcggagctgc ttcgagaccg ccggctggct cagcgcgact
     2101 cgccggacca gttcgctgac cggctgctcg ccttcgcgca gcgcatcgag gagctcgcgc
     2161 cgggtcggcg tggccagtat ctcgagcgtg gtgctgactg ccatgcctct acataaccag
     2221 acggatatat aactgtcaag gcatgtctcc cgcgagccag acttgggccg gtctccatgc
     2281 ggatcctgta tctcgacgtc gacacgctgc gcgccgacca cgtcggttgc tacggctacc
     2341 accgcgcgac cacaccgaac atcgatgcat tggccgccga cggagtccgg ttcgcgaacg
     2401 tctacgcgtc ggatgtgccg tgcctgccga gccgcaccgc gctcatcacc ggtgcgttcg
     2461 ggatccgcaa cggtgtcgtg aaccacggcg gggccgcggc cgacctccga tcgctcggtg
     2521 ccgagcggga gttcgtcagc cggtgggcgt ggcactcgtg ggcgggactg ttctacaacg
     2581 cgggatggca caccgcgtcg ttgtcgagct tccccttccg gcactccgcg tcgtggtgga
     2641 acgcggggtt catggaggca atgaacctca tgcgcggcat gggtggcgag cgcgccgatc
     2701 acgtcttgcc gggcgcgctc gactggctcg accggcgcgg ccgcaacgac cgctggttcc
     2761 tgcacgtgca cctgtgggat ccgcacacgc cgtacaacac gccagccgac tacgggaatc
     2821 cgttcgcgtc cgacccggtg tccgcgtggc acaccgacgc agtgcgcgcg cacaactggt
     2881 cgctctcggg accgcattcg gcgcaggaac cctgggggtt ccggccggac gagtggggcg
     2941 atccaccacc gcgcgaaccg tgggacgcgt cgtcgatgga cgcggtcaag cagatcttcg
     3001 acggctacga cgtcggcatt cgctatgccg acgacgcgct cggcacgctg tgcaacaagc
     3061 tcgccgacat cggcgtgctc gacgacacgg cgatcctcgt ttccaccgat cacggcgagg
     3121 cgttcggtga gctcggcgtg tacgcggacc accaggccgc cgacgaagcg acgtgccaca
     3181 tccccgcggt gttgcggtgg cccggtctcg caccgaacgt gcacgacggg ctgcagtacc
     3241 acctcgacgt ggcggcgacg ctcgtcgacc tcgcgggtat caaggtcccg cggcgctggg
     3301 acggtgtgcc gatcgacatc ggtggcg
//
LOCUS       DASUGA010000722         3170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_388644, whole genome shotgun sequence.
ACCESSION   DASUGA010000722 DASUGA010000000
VERSION     DASUGA010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388644"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VFX21_14300"
     CDS             <1..888
                     /locus_tag="VFX21_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HEX5097193.1"
                     /translation="DDDLDALVDTFGSAAALAADAGFDFVDVKHCHGYLLHELLGAVD
                     RAGAFGGDFEHRTAFLRRVVERIRARTPALAIGVRLSAYDFVPFAAGADDVGTPDGDA
                     SVAAPFAFGADQSGVGVDLGGAHRFLKLCEELGIGLVCITAGSPYYNPHIQRPAFFPP
                     SDGYRPPNDPLIDAARMIAATRELAADHPRVAIVGSGYSYLQQWLPHAAQHAIRTGGA
                     ASVGIGRGMLSYPTLPADVLAGRPLQSALLCRTFSDCTTAPRNGLVSGCYPLDDFYKD
                     SPQRIELAAVKKATRHRTR"
     gene            914..3136
                     /locus_tag="VFX21_14305"
     CDS             914..3136
                     /locus_tag="VFX21_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020728883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEX5097194.1"
                     /translation="MAKRSIELRTCPLCEAMCGLELHVEEGRVALIRPDKDDVWSKGH
                     ICPKGTTLGHLHEDPDRVREPMIRDGDTWREATWDEAFARAEELIAGVLETHGRDAMS
                     VYIGNPTAHAFSLSRYVGLFIALANLPVIYSAGTVDQWPKNVSSMLMYGGMWWIPAPD
                     IARTQYWLVLGGNPQASQGSLLAHADVMGEIERIQDRGGKVVVVDPRRTGTADTADEW
                     VPIVPGTDAAFLLALCNVLFDDGLTTLGGLDDFVIGFDAVREIAREFTPERVEATTRI
                     PAATTRRIARDLAAAPHAAVYGRIGTCNQEFGTLASWLVDVVNIITGNFDTPGGSMFG
                     KPVAWGVNSLPNPEWADGVSFGRWKSRVRGTPEVLGQVPVSCLGEEIATPGPGQIKGL
                     VTIAGNPVISAPGAGVLDDALPMLECMISVDNYLNETTRHAHVIFPGHSSLEQPHFDD
                     LIPMWQTRSTGNFSPPVFPRDDRPGEWEVLVRLAALLAGMTSADTDVPGIDDGYFMAL
                     CEMQGVDATHALSHYDPASPYGRGPLRMLDLQIRTGPFGDRYGDVPDGLTLQHFRDQP
                     HGIDLGPMVPRVREIIATKSGKIELAPQYITADVTRLAARLDRADESLVLVSRRHLRS
                     NNSWMHNVKVLVKGKDRCTLLVHPDDAKAAGVLDGALARVTSEAGEVEVPVEVTDEMM
                     RGVVCLPHGWGHDKPGTRLSVAREHAGVNNNLLAPGRLIDPLSNNAIVNGIPVEIAPA
                     "
BASE COUNT          494 a         1107 c         1070 g          499 t
ORIGIN      
        1 gacgacgatc tcgacgcgct ggtcgatacc ttcgggtcgg cggccgcgct ggcggccgac
       61 gcgggcttcg acttcgtcga cgtcaagcac tgccacggtt acctgttgca cgagctgctg
      121 ggcgcggtgg accgcgccgg cgcgttcggt ggcgacttcg agcaccgcac cgcgttcctg
      181 cgacgtgtcg tcgagcggat ccgggcgcgt actcctgccc tcgcgatcgg agtgcgcttg
      241 tcggcgtacg acttcgtgcc cttcgcggcg ggcgccgacg acgtcggtac accggacggt
      301 gatgcgagcg tggccgcacc gttcgcgttc ggcgccgacc agtccggcgt cggtgtcgac
      361 ctcggcggcg cacatcgctt tctgaagttg tgtgaagagc tcggcatcgg gctggtgtgc
      421 atcaccgccg gcagtccgta ctacaacccg cacattcaac gaccggcgtt cttcccgcca
      481 tccgacggct accgcccgcc caacgatccg ttgatcgacg cggcgcgcat gatcgcagcg
      541 acgcgtgagc tcgcggcgga ccatccgcgg gtagcgatcg tcggatcggg ttactcgtat
      601 ctgcagcagt ggttgccgca cgcggcgcag cacgcgatcc ggacgggcgg tgcggcgtcg
      661 gtcgggatcg ggcgcggcat gctgagctat ccgacgctgc cggccgacgt tctcgcgggc
      721 cggccgctgc agtccgcgtt gctgtgccgg acgttcagcg actgcacgac tgcgccgcgc
      781 aacgggctcg tctccgggtg ctatcccctc gacgacttct acaaggactc accgcagcgc
      841 atcgagctcg cggcagtgaa gaaggcgacg cgacaccgga cgcgttgacg ctcgtcatgc
      901 cagaatcggc ggcatggcga agcgttcgat cgaactgcgc acgtgcccgc tgtgtgaagc
      961 catgtgcggc ctggagttgc acgtcgagga gggccgcgtc gcgctgatcc gccccgacaa
     1021 agacgacgtg tggagcaaag gccacatctg tccgaagggc acgacgctcg gtcacctgca
     1081 cgaggacccg gaccgcgtgc gcgagccgat gatccgcgac ggcgatacgt ggcgcgaggc
     1141 gacgtgggac gaggcgttcg cgcgcgccga ggagctgatc gccggtgtgc tcgagacgca
     1201 tggccgcgac gcgatgtcgg tgtacatcgg caacccgacc gcgcacgcgt tctcgttgtc
     1261 gcgctacgtc gggctgttca tcgcgctcgc gaacctgccc gtgatctact cggcgggcac
     1321 cgtcgaccag tggcccaaga acgtctcgag catgttgatg tacggcggca tgtggtggat
     1381 tcccgcgccc gacatcgcac gcacgcagta ctggctcgtg ctcggcggca atccgcaggc
     1441 gtcgcagggc agcctcctcg cccatgccga cgtgatgggt gagatcgaac gcatccagga
     1501 ccgaggcggc aaggtcgtcg tcgtcgaccc ccgccgcacc ggtaccgcgg acaccgcgga
     1561 cgagtgggta ccgatcgtcc ccggcaccga tgccgcgttc ctcctcgcgc tgtgcaacgt
     1621 cttgttcgac gatggactta caacactcgg tggcctcgac gacttcgtca tcggtttcga
     1681 cgccgtgcgc gagatcgcgc gcgagttcac gccggagcgc gtcgaggcga cgacacgcat
     1741 ccctgccgcg accacccggc gcatcgcgcg cgatctcgcg gcggcgccgc acgccgccgt
     1801 ctacggccgg atcggcacgt gcaaccagga gttcggcacg ctcgcgagct ggctcgtcga
     1861 cgtcgtcaac atcatcaccg gcaacttcga cacgcccggc ggttcgatgt tcggcaagcc
     1921 cgtcgcgtgg ggcgtgaact cgctcccgaa tcccgaatgg gccgacggcg tgtcgttcgg
     1981 ccgctggaag tcgcgtgtgc gcggcacacc ggaagtgctc gggcaggtgc cggtctcgtg
     2041 cctcggcgag gagatcgcca cacccgggcc gggccagatc aaaggactgg tgacgatcgc
     2101 tggcaacccg gtcatctccg cgccgggcgc gggtgtgctc gacgacgcgc tgcccatgct
     2161 cgagtgcatg atcagcgtcg acaactacct caacgagacg acgcggcacg cgcacgtcat
     2221 cttcccgggc cactcgtcgc tcgaacagcc gcacttcgac gacctcatcc cgatgtggca
     2281 gacgcgcagc accggcaact tctcgccccc ggtcttcccg cgcgacgacc ggcccggcga
     2341 atgggaagtg ctcgtgcgcc tcgccgcgct gctcgcgggc atgacgagcg ccgacacgga
     2401 cgtgccgggc atcgacgacg gctacttcat ggccctgtgc gagatgcagg gcgtcgacgc
     2461 gacgcacgcg ttgtcgcatt acgaccccgc gtcaccgtac ggacgcggcc cactgcgcat
     2521 gctcgacctg cagatccgca ccggtccgtt cggcgaccgt tacggcgacg tgcccgacgg
     2581 cctgacgttg cagcacttcc gcgaccaacc gcacggcatc gacctcgggc cgatggtgcc
     2641 gcgcgtgcgc gagatcatcg cgacgaagtc cggcaagatc gagctcgcgc cgcagtacat
     2701 caccgccgac gtgacgcgcc tcgcggcccg gctcgatcgc gccgacgaga gcctggtgtt
     2761 ggtgtcgcgc cgtcatctcc ggtcgaacaa ctcgtggatg cacaacgtca aggtgctcgt
     2821 gaagggcaaa gaccgctgca ccttgctcgt gcacccggac gacgccaagg ccgcgggcgt
     2881 cctcgacggc gcgctcgcac gcgtgacatc cgaagcgggc gaggtggaag tgccggtcga
     2941 agtgaccgac gagatgatgc gcggtgtcgt gtgcctcccg cacggttggg gccacgacaa
     3001 gcccggcacg cgcctgtccg ttgcgcgcga gcacgcgggc gtgaacaaca acctgctcgc
     3061 gccgggccgc ctcatcgacc cgctgtcgaa caacgcgatc gtcaacggca tccccgtcga
     3121 gatcgccccg gcgtagtcgg gcgtcttaca agggcgaatt ccgcccttgt
//
LOCUS       DASUGA010000723         2278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_109136, whole genome shotgun sequence.
ACCESSION   DASUGA010000723 DASUGA010000000
VERSION     DASUGA010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109136"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VFX21_14310"
     CDS             complement(<1..370)
                     /locus_tag="VFX21_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase"
                     /protein_id="HEX5097195.1"
                     /translation="MRYRRMPIEIESPEQLGYDTITNNLSESSVADRRLRDLGIDVDL
                     DELLFCYGDHLGDPRLREAVAALGDGLRADDVLVTTGAAAALFATATSLLERDDHVVV
                     VRTNYATNLETPRAIGAAVDV"
     gene            complement(381..1196)
                     /locus_tag="VFX21_14315"
     CDS             complement(381..1196)
                     /locus_tag="VFX21_14315"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5097196.1"
                     /translation="MLNIGSFARLGQVSPRTLRHYDELGLLRPERIDPATGYRSYSVR
                     QLGRLHQLLALRDLGFSLEQIREMVDREPSLEEMRGMLRLREAQIAESVAEEQARLRR
                     VEAHIRALERNPTMAATTVVIKQTSPIRLAEATGVAIGFGNENLHPLFGELLPLVAQH
                     VMQQGANLGISVAHYEDVAENGSIVLHAGFDIGEQAVTGNDQVTIVELPVIEVASVVH
                     RGGMENVTETYEALMRWIEDSGFRATGYSRELYHEWTEDPAGRVTEMQMPIAR"
     gene            1284..2210
                     /locus_tag="VFX21_14320"
     CDS             1284..2210
                     /locus_tag="VFX21_14320"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HEX5097197.1"
                     /translation="MGRDRVHYKGMMSDSARWDAITLRADDIIITTPAKCGTTWTQMI
                     CALLILQTTTFDQPLGVISPWPDMLTRNIDDIVADLDAQRHRRFIKSHTPLDGLPDEP
                     GITYIGVGRDPRDACLSWDNHASNNDAIAMFTARHYAVGLDDIADELAKGPPERPEDP
                     MERFWNWMDDDASPNEAFNLAGLVHHLSQMWARRHEPNVILLRYEDLEADLEGEMRRL
                     AARLGVAVDEPRWPELVAAASFANMRANADTIAPESSQKLWHDNRQFFNTGHSGGWQD
                     LLDSDDVARYARRVRSLADPELVEWLHPAGLA"
BASE COUNT          375 a          751 c          750 g          402 t
ORIGIN      
        1 cgacgtcgac cgcggcgccg atcgcgcgcg gcgtctcgag gtttgtcgcg tagttcgtgc
       61 gtacgacgac cacgtggtcg tcgcgctcga ggagcgatgt tgcagtcgcg aacagcgccg
      121 cggccgcgcc cgtcgtgacg agcacgtcgt ccgcgcgcag gccgtcgccc agggcggcga
      181 cggcctcgcg caagcgcgga tctccgaggt ggtcgccgta gcagaacagc agctcgtcga
      241 ggtcgacgtc gatgccgaga tcgcggagcc gccggtccgc gaccgagctc tcggagaggt
      301 tgttggtgat tgtgtcgtag ccgagttgtt ccggcgactc gatctcgatc ggcatccgcc
      361 ggtagcgcat gcctggatgt ctagcgcgcg atcggcatct gcatctcggt cacgcggccc
      421 gcggggtctt cggtccactc gtggtacagc tcacggctgt atcccgtcgc gcggaagccg
      481 ctgtcttcga tccaacgcat caacgcttcg tatgtctccg tgacgttctc catgccgccg
      541 cggtgcacga cggacgcgac ttcgatcacg ggtagctcga cgatcgtcac ctgatcgttg
      601 cccgtgactg cttgttcgcc gatgtcgaag ccggcgtgca ggacgatgct gccgttctct
      661 gcgacatctt cgtagtgcgc gaccgagatg ccgaggttcg cgccttgttg catcacgtgt
      721 tgggcgacca gcggcaacaa ttcgccgaac agcggatgca agttctcgtt gccgaatccg
      781 atcgcgacgc cggtcgcttc cgcgagacgg atcgggctgg tctgcttgat gacgacggtt
      841 gttgcggcca tcgttgggtt cctttcgagc gctcggatgt gcgcttcgac ccggcgcagt
      901 cgcgcctgtt cctcggcgac actctcggcg atctgcgctt cccgcaaccg cagcatcccg
      961 cgcatctctt cgagcgaggg ctcgcggtcg accatctcgc ggatctgctc gagactgaag
     1021 ccgagatccc gcaaggccag cagctgatgc agccggccga gctggcgcac gctgtaggac
     1081 cggtagcccg tcgcaggatc gatgcgttcc ggtcgcagga gccccagctc gtcgtagtga
     1141 cgaagcgttc ggggcgagac ctgaccgagc cgggcaaagc ttccgatgtt caacatgacc
     1201 cgtacatcga tacgccttca cgcgacgtca aggtcaagcg tctcttcaca aggtgatcgt
     1261 acggtcgcta gcgtcgcggc gcgatgggcc gggaccgggt gcactacaag gggatgatgt
     1321 ccgacagcgc gcggtgggac gcgatcacct tgcgcgccga cgacatcatc atcacgacgc
     1381 ccgcgaagtg tggcacgacg tggacacaga tgatttgcgc gttgctgatc cttcagacga
     1441 cgacgttcga tcaaccgttg ggcgtcatct cgccgtggcc cgacatgttg acgcgcaaca
     1501 tcgacgacat cgtcgccgac ctcgacgcgc aacggcaccg gcggttcatc aagtcgcaca
     1561 cgcctctcga cggtctgccc gacgagccgg gcatcaccta cattggtgtt ggacgtgacc
     1621 cgcgtgacgc gtgcctgtcg tgggacaacc acgcgtcgaa caacgacgcg atcgcgatgt
     1681 tcaccgcccg ccactacgcc gtggggctcg acgacatcgc ggacgagttg gcaaagggcc
     1741 cgccggagcg gcccgaagac ccgatggagc ggttctggaa ttggatggac gacgacgcga
     1801 gtcccaacga ggcgttcaac ctcgccggcc tcgtccacca cctctctcag atgtgggcgc
     1861 gccgccacga gccgaacgtc atcctgctgc gctacgagga cctcgaagcc gatctcgagg
     1921 gggagatgcg ccgtctggcc gcgcgtctcg gggtcgcggt cgacgaaccg cgatggccgg
     1981 agctggtcgc agccgcgtcg ttcgcgaaca tgcgcgccaa cgccgacacg atcgcacccg
     2041 agtcctcgca gaagctatgg cacgacaacc ggcagttctt caacaccgga cacagtggcg
     2101 gctggcagga tctcctcgac agtgacgacg tcgcgcgcta cgcgcgccgg gtccggtcct
     2161 tggcggatcc cgaactcgtc gaatggctcc atccggccgg gttggcatag gtcgggccgg
     2221 ggccggtgag ttccggcgcc gcatacagtc gtacccgtca gtcgtcggca cgacgagg
//
LOCUS       DASUGA010000724         2212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1463378, whole genome shotgun sequence.
ACCESSION   DASUGA010000724 DASUGA010000000
VERSION     DASUGA010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463378"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VFX21_14325"
     CDS             <1..648
                     /locus_tag="VFX21_14325"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5097198.1"
                     /translation="TVTVCKAALDAGVRRVVYASSSSVYGGATRVPTPEGEPTFPRSP
                     YAVTKLAGEHYCRVFTELYGLETVALRYFNVFGARQRPDAAYAAVIPLFIDAVRRGDA
                     PIVHGDGTQSRDFTYIDDVVRANLCAAHAPAAQCAGHAYNIAGGESTSLLDILASLSR
                     IFEREVTPEFTEPRAGDVYRSLGDPTAAARDLGFRCEIPFEGGLRRTVEWLVSRD"
     gene            762..2066
                     /locus_tag="VFX21_14330"
     CDS             762..2066
                     /locus_tag="VFX21_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007450381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX5097199.1"
                     /translation="MAAPLAQLLRKLDERQAKVAVVGQGYVGLPVAMRASELGFRVVG
                     YDLDPERIDALRTGRSYVEDVTDEQLTTALDAGYWPTSDPLDLRAFDVAIVSVPTPLR
                     DGAPDLTYIESAGRDVAKVLHPGALVVLESTTYPGTTEELLRPLLEAGGLRAGEDFFL
                     GYSPERIDPGNAHWSFVTTPKVVAGVDDASREAIEAFYGALVEKVVPVATTAEAELVK
                     LLENTFRHVNIALVNELAMFARDLGVDIWSAIDAAATKPFGFMRFTPGPGVGGHCLPI
                     DPSYLAWRVERRLGQHFRFVELANEVNSHMADYVVSRVTAMLNEQARAVKGSRILLLG
                     LAYKAGTSDWRESPSMVVAEQLSALGAHVRAHDAHVPTDPALGPPVERAGCDPDELAA
                     ADLVIVLVDHPDLPYDDIAEHARLVLDTRARLRGRDFNGEVL"
BASE COUNT          325 a          791 c          758 g          338 t
ORIGIN      
        1 accgtgaccg tgtgcaaggc cgcgctcgat gcgggcgtgc ggcgcgtcgt gtacgcatcg
       61 tcgagcagcg tgtacggcgg cgcgacacga gtgccgacgc ccgagggcga gcccacgttc
      121 ccgcgctctc cctatgcggt cacgaaactg gccggggagc actactgccg cgtcttcacc
      181 gagctgtacg gcctcgagac cgtcgcgctg cgctacttca acgtgttcgg cgcccgccaa
      241 cgccccgacg ccgcctacgc ggcggtcatc ccgctcttca tcgacgcggt gcgccgcggc
      301 gacgcgccga tcgtgcacgg cgacggcacc cagagccgcg acttcacata catcgacgat
      361 gtcgtgcgcg ccaacctgtg cgccgcgcac gcgccggccg cacagtgcgc gggccacgcg
      421 tacaacatcg cgggcggcga gtcgacgtcg ctgctcgaca tcctcgcctc gttgtcgcgc
      481 atcttcgagc gcgaggtcac gccggagttc accgagcccc gggccggcga cgtgtaccgc
      541 agcctcggcg acccgactgc ggcggcgcgc gatctgggtt tccggtgcga aattccgttc
      601 gagggcggtt tgcgccggac cgtggagtgg ctcgtttccc gcgattgagc acttcggtgt
      661 gaagccgcac gacccggggc agaacgccac gggatgattg tgcggacgca gcggaggcct
      721 tccggccgca ggccactcgg ctcggagcgg agggagccgt agtggctgca ccgctcgcgc
      781 aattgctgcg caaactcgac gaacggcagg caaaggtcgc cgtggtcggt cagggatatg
      841 tcgggctgcc ggtggcgatg cgcgccagcg agctcgggtt ccgggtcgtc ggctacgacc
      901 tcgaccccga acgcatcgac gcgctgcgca ccggccgttc ctacgtcgaa gacgtcaccg
      961 acgagcagct caccaccgcc ctcgacgccg ggtactggcc gaccagcgac ccgctcgacc
     1021 tgcgcgcgtt cgacgtcgcg atcgtctcgg tgccgacacc gttgcgcgac ggcgcacccg
     1081 acctcaccta catcgaatcc gcgggccgcg acgtcgcgaa ggtgctgcat cccggcgcgc
     1141 tcgtcgtgtt ggagtcgacg acgtaccccg gcacgaccga agagctgctg cgcccgttgc
     1201 tcgaagcagg cgggctgcga gcgggcgaag acttcttcct cggctactcc cccgagcgca
     1261 tcgaccccgg caacgcgcat tggagcttcg tgacgacacc gaaggtcgtc gcgggtgtgg
     1321 acgacgcgtc gcgggaagcg atcgaggcgt tctacggcgc gttggtcgag aaagtcgtgc
     1381 cggtcgcgac gacagcggaa gccgaactgg tcaagctgct cgagaacacg ttccgccacg
     1441 tgaacatcgc gctcgtcaac gagctcgcga tgttcgcacg cgatctcggt gtcgacatct
     1501 ggtcggcgat cgacgcggcc gccaccaagc cgttcgggtt catgcgcttc acgcccggtc
     1561 ccggcgtggg cggccactgc ctgccgatcg acccttcgta tctcgcgtgg cgcgtcgaac
     1621 gccgcctcgg ccagcacttc cgcttcgtcg agctggccaa cgaagtcaac tcgcacatgg
     1681 ccgactacgt cgtgagccgc gtgacggcga tgctcaacga acaagcgcgc gccgtgaagg
     1741 gctcacggat cctgctcctc ggcctggcgt acaaggcggg cacgtcggat tggcgggagt
     1801 cgccgtcgat ggtggttgcg gagcaactgt cggcgctcgg cgcccacgtg cgcgcgcacg
     1861 acgcgcacgt gccaacggac cccgcactcg gcccgcccgt cgagcgggcc gggtgcgatc
     1921 ccgacgagct ggccgcggcc gacctcgtga tcgtgctcgt cgaccatccc gacctgccgt
     1981 acgacgacat cgccgagcac gcgcgcctcg tcctcgacac gcgcgcccgc ctccgcggcc
     2041 gcgacttcaa cggcgaggtg ctgtagcgcc tacaggtaca tgcccggcgg gccgtcgtgg
     2101 cggcgggtgt tcggctcggg gatgtcggcc gcttcgagca tgacgagcgc ggcgcggtcg
     2161 gtcgtgccgg cttccacgag ccggttcgcc tggcggctca tcgcgtcctc ga
//
LOCUS       DASUGA010000725         3515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_528664, whole genome shotgun sequence.
ACCESSION   DASUGA010000725 DASUGA010000000
VERSION     DASUGA010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528664"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="VFX21_14335"
     CDS             complement(<1..1027)
                     /locus_tag="VFX21_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5097200.1"
                     /translation="MIDTVPALLRERAAARGDHALLICDDDVLTYTDADSRSAALAHT
                     LLAWGAGPGTHVGLLYPNGPDFVVAWLAAARIGAITIPLSTFSTPTELVTLLRSADIE
                     LLLATKPFRGRDYTADVPELPNLDTPAMRRIVFEVPTVSGDDDRRRALEASVRPSDRM
                     VIVHTSGSTSEPKGVIHQHGALIGHLRNLNALRRYTEGDVLFSNSPFFWIGGFAYSLL
                     GTLLAGATLVCSNATDPSATLDLLERVRPNMVNGFAAAVAHLAKEPSFARRDLSSIRR
                     GNLWPIMPADVRAVDPELRHNMLGMTETGSVCLANADETDQPEARRGSFGKPVPGFEA
                     KIVDPETG"
     gene            complement(1024..1662)
                     /locus_tag="VFX21_14340"
     CDS             complement(1024..1662)
                     /locus_tag="VFX21_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097201.1"
                     /translation="MERWTFGVEPLPQATELTPLLRRVLGMVQSLEHDDPAVARLTGE
                     LRATARALTLRLPADMTPRLGDDAPADRRVYVDHARDVGAFNPCFPEYTIAVDGNRAT
                     GTVTFPLLFEGPPGLVHGGVLATFFDCVMQHHSCDVGVAGKTTSLLVEYRRPTPLDTQ
                     LSYEIERTTDDRRITSRARLFADDDTLCTATMEAVAGEFGRLPAVSPRRPAP"
     gene            complement(1662..3122)
                     /locus_tag="VFX21_14345"
     CDS             complement(1662..3122)
                     /locus_tag="VFX21_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018637199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX5097202.1"
                     /translation="MTDALPALSRHIAAVLVLDPSVPALEYERVWWTWGDIAATADAV
                     APHVGPGDRVAVLLRNHPPQVGLFVGLLRAGACVVTVNPDRGEERVRADLASLGVDTV
                     AGERADLEALAPAARWFVTDKLGAVEVLKTRGGGTQKNPGVAVEMLTSGTTGPPKRVP
                     LSYEMFSKVLEGTKYFQRKRDDDVRLRSGVVIINSPMVHLGGLFRVLTCLNEGRSFCL
                     LERFRVDDWVDAVRRHRPRTVGLVPAALRMVLDADVDPVDLESVRSVTSGTAPLAPED
                     ADAFIARFGVPVLTTYAATEFGGSVAGWNLDDHRQFWATKRGSVGRAQPGCALRVVDT
                     ESGASVGADEEGLLEVCAPQLGDGTTWTRTTDLARIDADGFVWILGRADQAIIRGGFK
                     IRPEDVRVALERDARVSGAAVVSKPDARLGAVPVAAVELRPGAPATTPDDLLAAAANV
                     LARYELPAELRVVAELPRTPSGKVDLTAVRELMGVA"
     gene            complement(3122..>3515)
                     /locus_tag="VFX21_14350"
     CDS             complement(3122..>3515)
                     /locus_tag="VFX21_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5097203.1"
                     /translation="TFFDSHVTYGLVSALEPVGLMRKVGLAETLRIALSGNDERVTAE
                     TALRISLVTEVVSRDELWARAHEIAAGIARKPTSATQGTVRAIWESLDRPYRAAMQQG
                     LIYTRLGNPIGQAEVAAKGADRPEPRQR"
BASE COUNT          530 a         1242 c         1237 g          506 t
ORIGIN      
        1 cgcccgtttc cgggtcgacg atcttcgcct cgaagccggg caccggcttc ccgaaggacc
       61 cgcgccgcgc ttcgggctgg tcggtctcgt cggcgttcgc caggcacacg ctgccggtct
      121 cggtcatgcc gagcatgttg tgccgcagct cgggatcgac ggcgcgcacg tcggcgggca
      181 tgatcggcca caggttgccg cgccggatcg agctgagatc acggcgcgcg aacgacggtt
      241 ccttggcgag atgcgcgacc gcggccgcga acccgttcac catgttcggg cggacgcgtt
      301 cgagcagatc cagcgtggcc gacgggtcgg tcgcgttgga acagacgagg gtcgcaccgg
      361 cgagcaacgt gccgaggagg ctgtacgcga agccgccgat ccagaagaac ggcgagttgg
      421 agaacagcac gtcgccttcc gtgtaacgcc ggagcgcgtt gaggttgcgg aggtgaccga
      481 tcagcgcgcc gtgctggtgg atgacgccct tgggttcgct ggtcgaaccc gacgtgtgga
      541 cgatcaccat gcggtccgag ggccgcacgc tcgcctcgag tgcgcgccgc cggtcgtcat
      601 cacccgaaac cgtcggcacc tcgaacacga tgcgacgcat tgcgggcgtg tcgaggttcg
      661 ggagctccgg cacgtcggcg gtgtagtcgc ggccccggaa cggcttcgtc gcgagcaaca
      721 gctcgatgtc ggcgctgcgc aacagcgtca ccagttcggt cggcgtcgag aacgtgctga
      781 gcgggatcgt gatcgcgccg atgcgggccg cggcgagcca ggcgacgacg aagtcaggac
      841 cgttcgggta cagcaggccg acatgcgtgc ccggcccggc accccacgcg agcaaggtgt
      901 gcgcgagcgc cgccgaacgc gagtcggcgt cggtgtacgt cagcacgtcg tcgtcacaga
      961 tgagcagcgc gtggtcgccg cgcgcggcgg cccgctcgcg cagcagtgcc ggcacggtgt
     1021 cgatcacggc gccggccgcc ggggtgagac ggccggaagc cggccgaatt cgcccgccac
     1081 cgcttccatc gtcgccgtgc acaacgtgtc gtcgtccgcg aacaagcgag cccgggacgt
     1141 gatgcggcga tcatcggtcg tacgttcgat ctcgtacgac aactgggtgt cgagcggcgt
     1201 cggccgccgg tactcgacca gcagcgacgt ggtcttgccc gccacgccga cgtcgcagct
     1261 gtgatgctgc atgacgcagt cgaagaacgt cgcgagcaca ccgccgtgca cgagacccgg
     1321 tggaccttcg aacagcagcg ggaacgtgac cgtcccggtg gcccgattgc cgtcgaccgc
     1381 gatcgtgtac tcggggaagc acgggttgaa cgcgccgaca tcgcgcgcgt ggtcgacgta
     1441 gacgcgccgg tcggcgggcg cgtcgtcgcc gaggcgcggc gtcatgtccg cgggcagtcg
     1501 aagggtcaat gcccgcgcgg tcgcgcgcag ctcgccggtg agccgcgcga cggccgggtc
     1561 gtcgtgttcg agcgactgca ccatgccgag cacgcgccgg agcagcggcg tcagctcggt
     1621 cgcctgcggc agtggttcga cgccgaaggt ccaccgctcc atcacgcgac acccatcagt
     1681 tcgcgcacgg cagtgagatc gaccttgccg gacggcgtgc ggggcagctc ggcgacgacg
     1741 cgcaactcgg ccggcagctc gtagcgcgcg agcacgttcg ccgcagccgc caacaagtcg
     1801 tcgggcgtcg tggccggtgc accgggccgc agctcgactg ccgcgacggg caccgcgccg
     1861 aggcgcgcgt cgggcttgct gacgacggcc gcaccgctca cacgcgcgtc acgttcgagc
     1921 gcgacgcgga cgtcttcggg ccggatcttg aacccgccgc ggatgatggc ctggtcggcg
     1981 cggccgagga tccacacgaa cccgtcggca tcgatgcgcg cgagatcggt ggtgcgcgtc
     2041 cacgtggtgc cgtcgccgag ctgcggcgcg cacacctcga gcaagccctc ctcgtcggcg
     2101 ccgaccgacg cacccgattc ggtgtcgacg acccgcaatg cacatcccgg ttgggcgcgc
     2161 ccgacgctcc cccgcttcgt cgcccagaac tgccggtggt cgtcgaggtt ccaacccgcg
     2221 acgctgcccc cgaactccgt tgccgcgtac gtcgtgagga cgggcacgcc gaaccgggcg
     2281 atgaacgcgt cagcgtcttc gggtgcgagc ggcgcggtgc ccgacgtcac cgagcgcacg
     2341 ctctcgaggt caacggggtc gacgtcggcg tccagcacca tacgcagcgc ggccggcacc
     2401 aagccgacgg tacgcgggcg gtgccgccgt accgcgtcga cccagtcgtc gacgcggaag
     2461 cgttcgagca ggcagaagga gcggccttcg ttcaggcacg tcagcactcg gaacaacccg
     2521 ccgaggtgca ccatcggcga gttgatgatg accacaccgg aacggagccg cacgtcgtcg
     2581 tcccgcttgc gctggaagta cttcgtgccc tccaagacct tcgagaacat ctcgtacgag
     2641 agcggcacgc gcttcggcgg ccccgtcgtc ccactggtga gcatctccac tgcgacgccc
     2701 ggattcttct gcgttccgcc gccgcgcgtt ttgagcactt cgaccgcgcc cagtttgtcg
     2761 gtgacgaacc agcgcgccgc gggcgcgagc gcttcgagat ccgcccgctc gcccgcgacc
     2821 gtgtcgacgc cgagcgatgc caggtcggcc cgcacgcgct cctccccgcg gtccgggttc
     2881 acggtcacga cgcacgcgcc cgcccggagc aggccgacga acagacctac ctgcggcgga
     2941 tggttgcgca acaacaccgc gacgcgatcg ccgggaccga cgtgtggcgc gacggcgtcg
     3001 gccgtcgccg cgatgtctcc ccaggtccac cagactcgtt cgtactccag cgcgggcacc
     3061 gacgggtcca gcacgagaac tgcggcgatg tgccggctga gcgcgggcaa cgcgtcggtc
     3121 atcagcgttg cctcggttcc ggacggtccg cgcccttggc cgcgacttcg gcctgcccga
     3181 tcgggttgcc gagccgggtg tagatgaggc cttgctgcat cgcggcgcgg taggggcggt
     3241 cgagcgactc ccatatcgcg cgcaccgtcc cctgcgtcgc cgaggtcggc ttgcgcgcga
     3301 ttcccgcggc gatctcgtgc gcacgcgccc acaactcgtc ccgcgagacg acttcggtga
     3361 cgaggctgat ccgcaatgcc gtttcggcag tcacgcgttc gtcgttcccc gacagggcga
     3421 tgcgcagcgt ctccgccagc cccaccttgc gcatcagccc gaccggctcg agcgcggaca
     3481 cgagcccgta ggtcacgtgc gagtcgaaga aggtc
//
LOCUS       DASUGA010000726         2648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_945065, whole genome shotgun sequence.
ACCESSION   DASUGA010000726 DASUGA010000000
VERSION     DASUGA010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945065"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VFX21_14355"
     CDS             complement(<1..279)
                     /locus_tag="VFX21_14355"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX5097204.1"
                     /translation="MKRLLVFGVILIAFAAGAGFVIGGMVDQHRPRRTPLSVAVDDGD
                     VNAVKSLLAHGADPNEGDEYLPPLFLAVGNDRVAIVRLLVDAGADPNRE"
     gene            complement(317..1168)
                     /locus_tag="VFX21_14360"
     CDS             complement(317..1168)
                     /locus_tag="VFX21_14360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX5097205.1"
                     /translation="MAGILALNKDAFGDVVDFLVREQARPERNIAYIGTERDAIAAEL
                     ADFEPGWAHAAHGVRDDRGRLVALAYGEWDTGLGRLWVHGPWVAGDDGAWEQWAKPVL
                     QAVIAAAPSIADIELSGTIENVRLGALANAIGFERSEPNYVLCVPLDGPTPERDPSVV
                     DATADDLAWITPLHDAEFPATYATPQQLLDGDGVFVAIDHGYAAGRVAPDGSGYIDFV
                     AVDGSARGAGRGRRLVQELVSRLAEAGAEREVCLTVQEHRVAARELYASLGFRIEIVI
                     VGYRRKE"
     gene            complement(1190..1711)
                     /locus_tag="VFX21_14365"
     CDS             complement(1190..1711)
                     /locus_tag="VFX21_14365"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5097206.1"
                     /translation="MISAVLFDLDDTLFPHEGWVDDAARIAPYDGVREGLDELTALVP
                     VGIVTDGDPRIQRAKLNALDLRVKLFVCSDEIGREFRKPHVFPFKRAAVVLRVRTSDC
                     VFVGDDPARDIAGAAAAGMRTVRVRTGQYAQQPDQVAPWRAEDTAAEAIQMLVTRIRS
                     SDGPSAERRTQQM"
     gene            complement(1799..>2648)
                     /locus_tag="VFX21_14370"
     CDS             complement(1799..>2648)
                     /locus_tag="VFX21_14370"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5097207.1"
                     /translation="DTDALVFTTWRLWLSLVPLSAIVWFRSRGADATPFKPVDITTGH
                     WLLVLFGAGAFFVSGAATAFAALQMTTLLNVTLIGALSPVLVIAFAVVFLGERVKSAH
                     ALQGTIAIVATVVVAFAASGGGTWSLAGDLVAVVSLFLNSGWFLYGRIVRTNLTVDPF
                     ALMLGTMVTGAVILTPITLVVHGSLEISGKGLFYAVCVMVAGTSAHIFMIWAHRYVPT
                     TISSPLLLAEPPLVAAGAWLWFGESLNAVEILGSAVVVASLWGLVRSSDLEQVEHDVP
                     DPAAPA"
BASE COUNT          489 a          906 c          866 g          387 t
ORIGIN      
        1 ctcgcgattg ggatcggcgc cggcgtcgac cagcagccgg acgatcgcaa ctcggtcgtt
       61 gccaactgcg aggaagagcg gcggaaggta ctcgtcgccc tcgttcggat cggcgccgtg
      121 ggctaacagc gacttcaccg cattcacgtc gccgtcgtcg acggcaacgc tcaacggcgt
      181 acgacgcggg cggtgctgat cgaccatccc accgatcacg aaaccggcgc ccgccgcgaa
      241 tgcaatgagg atcacgccga acacgagcaa gcgtttcatg cccgccccga cgcttcgcgg
      301 cgcagatccc ttgcattcac tccttccggc gataccccac gatcacgatc tcgatgcgaa
      361 accccagcga tgcgtacaac tcccgcgccg caacgcggtg ttcttgcacg gtcaggcaga
      421 cctcgcgttc ggccccagct tctgccaatc gcgagaccaa ctcctgcacg agacgacggc
      481 cgcgcccggc accgcgcgcc gagccatcga ccgcgacgaa atcgatgtag ccactgccgt
      541 cgggcgcgac gcgcccagcg gcgtagccgt gatcgatcgc gacgaacacc ccatcaccgt
      601 cgagcagttg ctgcggtgtt gcgtacgttg ccggaaactc ggcgtcgtgc aacggcgtga
      661 tccacgcgag atcgtcggcg gtggcatcga cgaccgacgg gtcccgctcg ggcgtcggcc
      721 cgtccaacgg aacgcagagg acgtagttgg gctcactccg ttcgaaaccg atcgcgttcg
      781 cgagcgctcc cagacgcacg ttttcaatcg ttcccgacaa ctcgatatcg gcgatcgaag
      841 gcgccgccgc aatgactgct tggagcactg gctttgccca ctgttcccac gcgccgtcat
      901 cgcctgcgac ccatggcccg tgcacccaca accggccgag accggtatcc cattcgccgt
      961 acgcgagggc aacgagccgc ccgcgatcgt cgcgcacacc gtgcgccgcg tgcgcccacc
     1021 caggttcgaa gtcggcgagc tcggccgcga tcgcgtcgcg ttcggtgccg atgtacgcga
     1081 tgttgcgttc gggtctcgct tgctcgcgga ccaggaagtc cacgacgtcg ccgaacgcat
     1141 ccttgttgag cgccaagatg cccgccacag cgggaaccgt agacgccggt tacatctgct
     1201 gggtgcgacg ctccgcgctg gggccatctg acgagcggat gcgcgtcacc agcatctgga
     1261 ttgcttcggc cgccgtgtcc tcggcgcgcc acggcgcaac ctgatcgggt tgttgcgcgt
     1321 attgaccggt gcgtactcgc acggtgcgca tcccggcggc cgccgcgccg gcgatgtcac
     1381 gcgccggatc gtcgccgacg aacacgcagt cggacgtgcg gacgcgcagc accaccgcgg
     1441 cgcgcttgaa ggggaagacg tgcggcttgc ggaactcgcg cccgatctcg tcgctgcaga
     1501 cgaagagctt cacgcgcaga tcgagggcgt tgagcttggc gcgctgaatg cgcgggtccc
     1561 cgtcggtcac gatcccaacg ggcacaagtg ccgtcagttc gtcgaggcct tcgcgcacgc
     1621 cgtcgtacgg cgcaatgcgc gccgcgtcat cgacccaacc ctcgtgggga aacaaggtgt
     1681 cgtcgagatc gaataggaca gcactgatca cgcgggtcgg cctccagccc gagagttcct
     1741 ccgtaggagg ctcgaccggc cggtggacga ccttaaggct ggcgatgcgg gagtccggtc
     1801 aggccggtgc ggccgggtcg ggcacgtcgt gttcgacctg ttccaggtca gagctgcgca
     1861 ccaggcccca gagcgacgcc acgacgaccg cggatcccag gatttcgacg gcgttcagcg
     1921 actctccgaa ccagagccag gcgcccgccg cgacgagggg tggttcggcg agcaacagcg
     1981 gcgacgagat cgtggtcggt acgtagcggt gcgcccagat catgaagatg tgcgcagacg
     2041 taccggcgac catcacgcag accgcgtaga acaggccctt ccccgaaatc tcgagtgagc
     2101 cgtgcacgac gagcgtgatc ggcgtgagga tgacggcgcc ggtgaccatc gtgcccagca
     2161 tcagcgcgaa cggatcgacg gtgaggttcg tgcgcacgat gcgtccgtag agaaaccagc
     2221 ccgagttcag gaagaggctg acgaccgcga cgagatcgcc ggccaggctc caggtaccgc
     2281 cgcccgatgc cgcgaacgcg accacgaccg tggcaacgat cgcaatcgtt ccctggagcg
     2341 cgtgcgcgct cttcacccgc tcgcccagga acaccaccgc gaatgcgatg acgagcaccg
     2401 gcgacagcgc accgatgagg gtgacgttca gcagtgtggt catctgcagg gccgcgaacg
     2461 cagtcgccgc accggagacg aagaacgcac cggcgccgaa caacacgagc agccaatggc
     2521 cggtagtgat gtcgaccggc ttgaagggcg tcgcgtcggc accgcgagaa cggaaccaca
     2581 cgatggccga gagcggaacc agggaaagcc agagccgcca ggtcgtgaac accagtgcgt
     2641 cggtgtcg
//
LOCUS       DASUGA010000727         3695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2020840, whole genome shotgun sequence.
ACCESSION   DASUGA010000727 DASUGA010000000
VERSION     DASUGA010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2020840"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="VFX21_14375"
     CDS             <1..1109
                     /locus_tag="VFX21_14375"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5097208.1"
                     /translation="GSIFLVNVPIVVVALIAGVFLIPTSKDPSKPPLDPVGALLSIVG
                     LVALVYAIIQAPEKGWTSPETVGVGAFGIVVLVAFALWERYTPSPMLDTSFWKNPRFS
                     AASAGIMLLFFAMFGSIFLLTQYLQFVMGYDALAAGIGLLPMALTMLVVAPNSPRLVE
                     RIGTKKTVGAGLTIAGFGLALMAMLPATDISYWGDVAWRLIVMAIGMGLTMAPATESI
                     MGSLPRAKAGVGSAVNDTTRQVGGALGVAIIGSVMASAFGSRVDTLFTNSGVPAEGVD
                     VAKEGLGQAIAVASDPRVPSSLSAQLIQGAKEAFVDGAHHGLWVAAAVALLGAAVVFK
                     WLPARELPAVDDPGAAIGAGRAPEAPVELETDPV"
     gene            1106..1702
                     /locus_tag="VFX21_14380"
     CDS             1106..1702
                     /locus_tag="VFX21_14380"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5097209.1"
                     /translation="MTDAVIESRRGPGRPRSAECDAAIMEATLVEYADAGFDGLSVDA
                     VAARAGCSKATIYRRYPSKLDLVITASRSVAEELAPELAGPTLRDDLDEICWSLARKL
                     QHPLFGRVMAMVVGDATRHDDVRAMHSDLVRMRRQPCIEALQRAVERGEMRDGVDLDA
                     AVDQLGGPLFYRHLVTRMPIDEAYVNELIDSFIASYGV"
     gene            complement(1712..>3695)
                     /locus_tag="VFX21_14385"
     CDS             complement(1712..>3695)
                     /locus_tag="VFX21_14385"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEX5097210.1"
                     /translation="NIVGKSYGQIFREFEGELDPNVPQGSGDVKYHVGATGKWTARSG
                     GDITLTLAANPSHLEAVDPVVEGMARAKQDRARDTTHEKVLPILVHGDAAFAGQGVVA
                     ETLNLSELPGYDVGGTVHIVVNNQVGFTTPPGFARSTVYATDIAKAVQAPIFHVNGDD
                     PEAAVRVIRLAFAFRRRFAKDVVVDMVCYRRYGHNEGDEPAFTQPRMYEIIGRRRSVR
                     KLYTETLVNRGDLTLQECEAALEDFRSRLEEAFVETHEQQHRPALSDERPDDPARPSV
                     DTGVSRERLAAIVDTLVTFPPGFAPHPKLERIIKGRRTEFDNDKIDWALAEQLAFGSL
                     LQQGAPVRIAGQDTRRGTFSQRHATVVDHATEAEYIALEHLADASAPFMIYDSVLSEF
                     AALGFEYGYSVADQDALVIWEAQFGDFSNGAQTIIDQFLVAAEDKWGQRSGLTMLLPH
                     GFEGQGPEHSSARLERFLVLCAQDNMRVVYPTTAAQYFHVLRRQVIDPDRKPLIVMTP
                     KRYLRMAQTYSPVSAFTDGRFELLLPDPASPDPASVRRLVFCTGKFGYELLARRDETN
                     APVAIVRLEQLYPWPEREIAEQLGRYANADVVWAQEEPGNMGARYFARRRIEEIASGR
                     PVGVVARASSPSPATGSSTVHDLEQKRLLEQALTID"
BASE COUNT          527 a         1235 c         1300 g          633 t
ORIGIN      
        1 ggggctcgat cttcctcgtg aacgtgccga tcgtcgtcgt ggccctcatc gccggcgtct
       61 tcttgatccc gacctccaag gacccgagca agccaccgct cgacccggtg ggcgcgctgc
      121 tgtcgatcgt cggtctcgtg gccctcgtct acgcgatcat ccaggcgccc gagaagggct
      181 ggacctctcc cgagaccgtc ggcgtgggcg cgttcggcat cgtcgtgctg gtcgccttcg
      241 cgttgtggga gcggtacacg ccgagcccga tgctcgacac gagcttctgg aagaacccgc
      301 gtttcagcgc ggccagcgcg ggcatcatgc tgctcttctt cgcgatgttc ggctcgatct
      361 tcttgctgac ccagtacctg cagttcgtca tgggctatga cgcgctggcg gccgggatcg
      421 ggctcctccc gatggcgctc acgatgctgg tcgtggcacc gaacagcccg cgcctcgtcg
      481 agcggatcgg cacgaagaag accgtcggcg cggggctgac gatcgccgga ttcggtctcg
      541 cgctgatggc gatgctgccc gccaccgaca tctcgtactg gggtgacgtc gcgtggcgcc
      601 tgatcgtcat ggcgatcggt atgggtctca cgatggcgcc cgcgaccgag tcgatcatgg
      661 gctcgctccc gcgggccaag gcgggtgtgg gttccgcggt gaacgacacg acgcgtcagg
      721 tcggcggcgc gctcggcgtc gccatcatcg gcagcgtcat ggcgtccgcg ttcggctccc
      781 gcgtcgacac gttgttcacc aacagcggcg ttccggccga gggcgttgac gtcgccaaag
      841 aaggtctcgg tcaggccatc gcggtcgcga gcgatccgcg ggtgccgagc tcgttgagcg
      901 cgcagttgat ccaaggcgcc aaggaagcgt tcgtcgacgg agcacaccac ggcttgtggg
      961 tagcagccgc ggttgcactc ctcggcgcgg cggtcgtgtt caagtggctg ccggcgcgcg
     1021 agctgccggc cgtcgacgac cccggtgcgg cgatcggcgc cgggcgcgcg cccgaggcgc
     1081 cggtcgagct cgaaacggat ccggtgtgac cgacgccgtc atcgagtcgc gtcgggggcc
     1141 gggccggccc cgcagtgcgg agtgcgacgc cgccatcatg gaggcgacgc tcgtcgaata
     1201 cgccgacgcg ggcttcgacg gtctcagtgt cgacgccgtc gccgcgcgcg ccggctgcag
     1261 caaggcgacg atctaccgcc gctacccgtc gaagctcgac ctcgtgatca ctgcgtcgag
     1321 gtcggtcgcg gaggagctcg cacccgagct cgcaggaccg acgttgcgcg acgacctcga
     1381 cgagatctgt tggagcctgg cccgcaagct gcagcacccg ctgttcggcc gcgtcatggc
     1441 gatggtcgtc ggcgacgcga cccgtcacga cgacgtgcgc gcgatgcact ccgatctcgt
     1501 gcgcatgcgg cgtcagccgt gcatcgaagc gctgcaacgc gcggtcgaac gcggcgagat
     1561 gcgcgacggc gtcgacctcg acgcggccgt cgaccagctc ggcggcccgc tcttctaccg
     1621 ccacctcgtc acccgcatgc cgatcgacga ggcctacgtg aacgagctca tcgactcgtt
     1681 catcgcgtcg tacggggtgt agttccgcga atcagtcgat ggtgagcgct tgttcgagca
     1741 ggcgcttttg ctcgaggtcg tggacggtgg agctgccggt ggcggggctg gggctcgacg
     1801 cccgggccac cacgccgacc gggcgaccac tcgcgatctc ctcgatgcgg cggcgggcga
     1861 agtagcgggc gcccatgttg cccggctcct cctgtgccca caccacatcg gcgttcgcgt
     1921 agcgcccgag ctgttccgcg atctcgcgct cgggccacgg atagagctgt tcgagccgca
     1981 cgatcgcgac cggcgcgttc gtctcgtcgc ggcgcgcgag cagctcgtaa ccgaacttgc
     2041 cggtgcagaa caccaatcgg cgtaccgacg cgggatccgg gctcgcaggg tccggtaaca
     2101 agagctcgaa gcgaccgtcg gtgaacgcgc tcacgggcga gtacgtctgc gccatgcgca
     2161 agtagcgctt cggcgtcatc acgatcaacg gcttgcgatc cggatctatc acctggcgcc
     2221 gcaacacgtg gaagtactgc gcggccgtcg tcggatagac gacgcgcatg ttgtcctggg
     2281 cacacagcac gaggaaccgt tcgagccgcg cggaggagtg ctcgggccct tggccttcga
     2341 agccgtgggg gagcagcatc gtcaagccgc tgcgctggcc ccacttgtct tcggcggcca
     2401 cgaggaactg gtcgatgatc gtctgcgcgc cgttggagaa gtcgccgaac tgcgcttccc
     2461 agatgacgag cgcgtcctgg tcggcgactg agtagccgta ctcgaagccg agcgcggcga
     2521 actcggagag caccgagtcg tagatcatga agggcgcgct cgcgtcggcg aggtgttcga
     2581 gcgcgatgta ctcggcctcg gtggcgtggt cgacgaccgt cgcgtggcgc tggctgaacg
     2641 tgccccgccg ggtgtcctgg cctgcgatgc gcaccggcgc accttgttgc agcagcgatc
     2701 cgaacgccag ctgttcggcc agtgcccagt cgatcttgtc gttgtcgaac tcggtgcgcc
     2761 ggcccttgat gatgcgttcc agcttcgggt gcggtgcgaa accgggcggg aacgtgacca
     2821 gcgtgtcgac gatcgcggcc agtcgttcac gcgacactcc ggtgtcgacc gacgggcgcg
     2881 cggggtcgtc gggccgctcg tcactgagcg cgggccggtg ctgttgttcg tgcgtctcga
     2941 cgaacgcctc ttcgaggcgc gagcggaagt cttcgagcgc ggcctcgcac tcttgcaacg
     3001 tcaggtcgcc gcggttgacg agcgtctcgg tgtacagctt ccgcaccgaa cggcggcggc
     3061 cgatgatctc gtacatgcgc ggttgcgtga acgcgggctc gtcgccctcg ttgtgcccgt
     3121 agcgccggta gcagaccatg tcgaccacga cgtccttggc gaaacgccgg cggaacgcga
     3181 acgcgagccg gatgacgcgc accgcggcct cggggtcgtc gccgttcaca tggaagatcg
     3241 gcgcctgcac ggccttcgcg atgtcggtcg cgtacacggt cgagcgcgca aagccgggtg
     3301 gcgtcgtgaa cccgacctgg ttgttgacga cgatgtgcac ggtgccgccg acgtcgtagc
     3361 cgggcagctc cgacaggttg agggtctcgg ccacgacacc ctggccggcg aacgcggcgt
     3421 cgccgtggac gaggataggc agcaccttct cgtgcgtcgt gtcgcgggcg cggtcctgct
     3481 tggcgcgcgc catgccctcg acgaccgggt cgaccgcttc gagatggctc gggttggccg
     3541 cgagcgtcag cgtgatgtcg ccgccggagc gcgcggtcca tttaccggtc gcgccgacgt
     3601 ggtacttcac gtcgccgctg ccctgcggca cgttcgggtc gagctcgccc tcgaactctc
     3661 ggaagatctg cccgtacgac ttgccgacga tgttc
//
LOCUS       DASUGA010000728         3771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_215277, whole genome shotgun sequence.
ACCESSION   DASUGA010000728 DASUGA010000000
VERSION     DASUGA010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215277"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VFX21_14390"
     CDS             <1..726
                     /locus_tag="VFX21_14390"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX5097211.1"
                     /translation="DCTHAGRAFATQLGRELAEVGVRVVSGLALGIDGAAHAGVLSVS
                     GGPPVGVVGSGLDVVYPRRHTELWERVATAGVLLTEHPLGAKPVGWHFLARNRIIAIL
                     AEVVVVVESHASGGALQTATEAGQRGRTVLVVPGSTQSVASEGTNALLRDGCGVCCDT
                     TDVLTALGLSGVRRPRPDARPAPSSTGEAVLRAMGGEPCSLEQIMLRSSLGLGELVAE
                     LDRLETDGWIVRRGAWCERVGPR"
     gene            787..1677
                     /locus_tag="VFX21_14395"
     CDS             787..1677
                     /locus_tag="VFX21_14395"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HEX5097212.1"
                     /translation="MAKADLWQLDGFVDALTASAPATVRAYRSDLASFVEWADRLHVT
                     DPANVNRKMLRRYLSYLHTRGYASRTLARKASSLRRYFDWLRRTGTIDASPAANLVAP
                     KASGRLPRVLRTDEVEVLLDDVPARADDDAAVRMRDDAVLELLYGSGLRIGELCSLDV
                     TDLDLDASIVRVWGKGGKQRQVPMSAPSVDAVRRWLESGRSSLANDETPLGAVFVNRK
                     GRRLTPRDARRILDRRASAPTNPHALRHTFATHLLDGGADLRAVQELLGHADLSTTQH
                     YTHVSKERLRHVLDATHPRA"
     gene            1686..2468
                     /gene="whiG"
                     /locus_tag="VFX21_14400"
     CDS             1686..2468
                     /gene="whiG"
                     /locus_tag="VFX21_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009068913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HEX5097213.1"
                     /translation="MEEQPPGQIDRLWTEYKSSGDREIRDQLIVHYSPLVKYVAGRVA
                     VGLPQNVEQSDLVSYGIFGLIDAIDKFDLARGYKFETYAIARIKGAILDELRSIDWVP
                     RSVRAKARSLEKAYAKLESELHRTPTDEELAAELDITDEQLQTMLSQISFVGLVALDE
                     MLSVGGDRGESLTLGDTIADGGEGPVQAYEVEEMRHILADAINRMPEREKIVLTLYYY
                     EGLTLAEIGQVLGVTESRVCQIHTKAVLQLRSKILAQEREPA"
     gene            2550..>3771
                     /locus_tag="VFX21_14405"
     CDS             2550..>3771
                     /locus_tag="VFX21_14405"
                     /inference="COORDINATES: protein motif:HMM:NF018018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX5097214.1"
                     /translation="MSRRVRRARRWRCACLPLVVGLLFASALPAAADTVSYRPPVDAP
                     VADPFRAPAHRYAAGNRGIDYATSTDMPVRAAADGEVIFAGPVAGTLHVTVLHADGLR
                     TSYSFLDRVEVSRGMRVAQGDAVGTASASTHFGVRDAQGEYLDPQLLFDGAVRMRVRL
                     VPGADEGEAPLVAAERRALLRLVLERGLELANASRVDVLAHYAVELRLDVRTARILWR
                     LADQAGVSCTPAGDVPPVPSGRVAVLVGGLGSTSTQAAIDRVDTDALGFGHADVVRFS
                     YAGGRVPDPTDDERYRGIAAREYEARDTTGDFESAAERLADLLVAMAAARPGVPIDVI
                     GHSLGGVVARLALAEAGARGRLPAEVATLVTLGSPHAGADLATAAVALDGRVGLATPR
                     ALETVVGIPPDAPAV"
BASE COUNT          555 a         1324 c         1291 g          601 t
ORIGIN      
        1 gactgcacgc acgccggccg cgccttcgcg acgcagctcg gtcgagagtt ggccgaggta
       61 ggcgtgcgcg tcgtgtcagg cctggcgttg ggcatcgacg gcgccgcgca tgcaggcgtg
      121 ctgtcggtgt ccggtggacc accggtaggt gtcgtcggga gcgggctcga cgtcgtctat
      181 ccgaggcggc acaccgaact gtgggagcgc gtcgcgactg cgggtgtgtt gctcaccgag
      241 cacccgctcg gtgccaaacc ggtcggatgg cacttcctcg ctcgcaatcg gatcatcgcc
      301 atcctcgccg aggtcgtggt cgtggtggag tcgcatgcca gcggtggagc cctacagacg
      361 gccaccgaag ccggccaacg cggccgcacc gtgctcgtcg tcccgggatc cacccagagc
      421 gtggcgtcgg aaggtaccaa cgccctgttg cgcgacggct gcggcgtgtg ctgcgacacc
      481 accgacgtgc tgaccgcgct gggcctgtcc ggtgtgcgcc gccctcggcc cgatgctcgt
      541 ccggcgccgt cgtcgaccgg cgaggccgtg ctccgcgcca tgggcggcga accgtgctcg
      601 ctcgagcaga tcatgctgcg gtcgagcctc ggcctgggcg agctggtcgc cgagctggat
      661 cggctcgaga cggacgggtg gatcgtgcga cggggtgcct ggtgcgaacg ggtcggtccg
      721 cggtgaggag cacgacgatg cggcgcaatc caattctcgc ggtgctcgcc cggtaccctg
      781 cttgtcgtgg ccaaggcgga tctctggcaa ttggatggtt tcgtcgacgc gctcaccgcg
      841 tcggcgccgg ccaccgtccg cgcctaccgg agcgacctcg cctcgttcgt cgagtgggcc
      901 gatcgcttgc acgtcaccga tccggcaaac gtcaaccgca agatgctccg gcggtatctc
      961 tcgtacttgc acacacgcgg ctacgcgtct cggacgctgg cgcgcaaggc ctcgtcgttg
     1021 cggcggtact tcgactggct gcgccgcacg ggcaccatcg acgcgagccc cgccgccaac
     1081 ctggtggcgc cgaaggcctc gggtcggctt ccgcgggtgt tgcgcaccga cgaggtcgag
     1141 gtgctgttag acgatgtgcc ggcgcgagcc gacgacgacg cggccgtgcg catgcgcgac
     1201 gacgcggtgc tcgaactgct ctatggaagt ggcttgcgca tcggcgagct gtgcagcctc
     1261 gacgtgaccg acctcgatct cgacgcgtcg atcgtgcgtg tttgggggaa gggcggcaag
     1321 cagcggcaag taccgatgag cgcgccctcg gtcgacgcgg tgcgccgatg gctcgagtcc
     1381 ggccgctcga gcctggcgaa cgacgagaca ccgctgggcg cagtgttcgt caaccgcaag
     1441 ggccggcgac tcacgccgcg cgacgcgcgt cgcatcctcg accgacgagc gagcgcaccg
     1501 accaacccac acgcgctgcg ccacacgttc gccacacacc tgctcgacgg tggggccgac
     1561 ctgcgcgccg tgcaggagct gctcggtcac gccgacctgt ccacgacgca gcactacact
     1621 catgtcagca aagagcggct ccgccacgtc ctggatgcca ctcacccgcg agcgtaggaa
     1681 cgatcttgga agagcagcca ccaggacaaa tcgatcgtct gtggactgag tacaagtcgt
     1741 ccggcgatcg cgagatccgt gaccagctga tcgtgcacta ctcaccgctg gtgaagtacg
     1801 tcgcgggtcg tgtggctgtc ggcctgccgc agaacgtcga gcagtccgac ctggtgagct
     1861 acgggatctt cggtttgatc gacgccatcg acaagttcga tctcgctcgt ggctacaagt
     1921 tcgagaccta cgccatcgct cgcatcaagg gcgcgatcct cgacgagctg cgctcgatcg
     1981 actgggtgcc gcggtcggtg cgcgccaagg cacgctcgct cgagaaggcg tacgccaagc
     2041 tcgagagcga gttgcaccgc acgcccaccg acgaggagct cgcggccgaa ctcgacatca
     2101 ccgacgagca actgcagacg atgctctcgc agatctcgtt cgtcggtttg gtcgcgctcg
     2161 acgagatgct gtcggtcggt ggcgatcgag gtgagtcgct cacgctcggc gacaccatcg
     2221 ccgacggcgg cgaggggccc gtgcaggcgt acgaggtcga agagatgcgt cacatcctcg
     2281 ccgacgccat caatcgcatg cccgagcgcg agaagatcgt cctcacgctc tactactacg
     2341 aaggtctgac gctggccgag atcggtcagg tgctcggcgt caccgagagc cgggtgtgcc
     2401 agatccacac caaggccgtg ctgcaactgc gttcgaagat cctggcccag gaacgcgaac
     2461 ccgcgtagcg catccgcatc gcgctcggcg caacctttcc ccctccgcgc acttccccct
     2521 cgtaccgccc atgaccaggg gaagaagcga tgtcgcgccg cgttcgccgc gcccgtcggt
     2581 ggcggtgcgc gtgcctgccg ctcgtcgtcg gtttgctgtt cgcatccgcg ctgccggcag
     2641 ctgccgacac ggtctcgtac cggccgcccg tcgacgcgcc agttgccgac cccttccgcg
     2701 cgccggcgca ccgctatgcg gcggggaatc gcggcatcga ctacgcgacc tcgacagaca
     2761 tgcccgttcg tgccgcggcc gacggcgagg tcatcttcgc cggccccgtg gccggaaccc
     2821 tccacgtcac ggtgctccac gccgacggac tccgaaccag ctacagcttt ctcgatcgcg
     2881 tcgaggtgtc acgcggcatg cgcgtcgccc aaggcgacgc cgtcggcact gcatcggcga
     2941 gcacccactt cggtgtgcgc gatgcccaag gcgagtatct cgatccgcag ctcctcttcg
     3001 atggtgcagt acggatgcgc gtgcgcctgg tgcccggcgc ggacgaaggt gaagcgccac
     3061 tcgtcgcagc cgagcggcgt gcgctgttgc ggctcgtcct cgagcgcggt ctggagctgg
     3121 ccaacgcgtc gcgcgtcgat gtgctcgcgc actatgcagt cgagcttcgc ctcgacgtgc
     3181 gcacggcacg catcctctgg cggctcgccg atcaggccgg cgtgtcgtgc acgccggccg
     3241 gcgacgtgcc tcccgttccg agcggacggg tcgcggtgct cgtcggtggg ttgggatcga
     3301 catcgacgca ggccgccatc gatcgggtcg acaccgacgc gctcggcttt ggccacgccg
     3361 acgtcgtgcg cttctcgtat gccggcggtc gggtgcccga cccaaccgac gacgagcgat
     3421 accgcggcat cgccgcccgc gagtatgaag cgcgcgacac gacgggtgat ttcgagtcgg
     3481 cggccgaacg cctcgccgac ctgctcgtgg cgatggccgc agcgcgcccg ggcgtgccca
     3541 tcgacgtgat cggccattca ctgggcggcg tcgtcgcccg cctggctctg gccgaggcgg
     3601 gagctcgcgg tcggttgccg gccgaggtcg ccacgctggt gaccctcggg agcccgcacg
     3661 caggagccga cctcgcaacc gccgcggtcg cgctcgacgg ccgggtgggc ctcgccaccc
     3721 ctcgtgccct cgagaccgtc gtaggcatcc cgcccgacgc gccggccgtc c
//
LOCUS       DASUGA010000729         2188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_76040, whole genome shotgun sequence.
ACCESSION   DASUGA010000729 DASUGA010000000
VERSION     DASUGA010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76040"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VFX21_14410"
     CDS             <1..590
                     /locus_tag="VFX21_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097215.1"
                     /translation="SALWKSQIPNAGPALATRLEVAISLGLGAGLATGAVIRMVRSGR
                     RSQPAAGDGDGRWLSLLVVGLSDSELARIAHDLDADEVLEVATRELAARLEPASHEFT
                     AGSLDVRVMAGDREHEWTLAAAGETVVAERGRGRAPAAVVHMTFPVALQLLAGTVPAA
                     DKRIRVDGDEAFYARIAPLVPERATPVADAPASAL"
     gene            complement(553..1185)
                     /locus_tag="VFX21_14415"
     CDS             complement(553..1185)
                     /locus_tag="VFX21_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097216.1"
                     /translation="MRRLLLFVLVAGATISATAGPATAHTCVTAARAPVGQSSTITLV
                     AAVEKEIPDIAFTLPAGLALDRVDPKPGWKATRAGSTVRYQGGPTAPLSCERFSVSVT
                     PARRGVYGIVAVQRDANGTVVARSTPVPGAAHVDPAEQRVYAGVEPPAPPSQTGGRSP
                     LLYVAGGLILLAFALMTFLFFRNRRIAREDELADRVEEFKVRTRERPPPE"
     gene            1249..1827
                     /locus_tag="VFX21_14420"
     CDS             1249..1827
                     /locus_tag="VFX21_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097217.1"
                     /translation="MRVRILIPVVVLSLTALGACGGSDKPKAESESTSTAPSPGGTAH
                     VAKAATDDPSVSAKMVCSDEAQEDIYKGATGVKPTKVTTPTWEDHVYACDFVYPNGTM
                     RLETKELADADATKKFFDEQAEKLGKKEPLTGIGEEGFSTDDGGAVIRKDYKVLTVDV
                     SKLPAQFGLPLSPRSDVAINTAFTIFQCWVGA"
     gene            complement(1831..>2188)
                     /locus_tag="VFX21_14425"
     CDS             complement(1831..>2188)
                     /locus_tag="VFX21_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5097218.1"
                     /translation="GVALMIVVALLPVPAPIAAAVVAWGVAGFGMGLAYAPISLVVLA
                     QAPEGGEGTATASLQLNDTLGIALGTGVAGAIVAAGDELGWSRGGALAIAFAICIAFA
                     LAAAAGAQRLPRRVEV"
BASE COUNT          362 a          752 c          761 g          313 t
ORIGIN      
        1 tctccgcgct gtggaagtca caaatcccca atgccggccc ggcgctcgcc acccgcctcg
       61 aagtcgcgat ctcgctcgga ctcggcgccg gcctcgcgac gggcgccgtg atccgaatgg
      121 tccgcagcgg acggcgttcc caacccgccg ccggcgacgg tgacggtcgc tggctctcct
      181 tgctcgtggt cgggctcagt gattccgagc tggcgcggat cgcgcacgat ctggatgccg
      241 acgaagtgtt ggaagtcgcg acccgcgagc tcgccgcgag gcttgaaccc gcgagccacg
      301 agttcaccgc cggttcgctc gatgtgcgcg tcatggccgg tgatcgcgaa cacgagtgga
      361 ccctggccgc tgccggcgag accgtcgtcg ccgagcgcgg ccgcggccgt gcgccggcgg
      421 ccgtcgtgca catgacgttc ccggtcgcgt tgcaactgct cgcgggaacg gtgcccgccg
      481 ccgacaagcg catccgcgtc gacggcgacg aagcgttcta cgcgcgcatc gcgccgctgg
      541 tgccggaacg cgctactccg gtggcggacg ctcccgcgtc cgcactttga actcctcgac
      601 ccggtcggca agctcgtctt cgcgagcgat ccgccggttg cggaagaaca agaacgtcat
      661 caaggcgaac gcgagcagga tcaggccgcc ggcaacatag agcagcggtg aacgccctcc
      721 ggtctggctc ggcggcgcgg gcggctcgac gcccgcgtac acacgttgct cggccgggtc
      781 gacgtgcgcc gcgcccggga ccggtgtcga gcgggcgacc actgtgccgt tcgcgtcgcg
      841 ttggaccgcg acgattccgt agacgccgcg gcgcgcgggc gtgacgctga ccgagaaacg
      901 ctcgcacgac agcggtgcgg tcggtccgcc ctgatagcga accgtcgatc cggcgcgggt
      961 tgctttccac cccggcttgg gatcgacgcg gtcgagtgcc aggcccgcgg gcaaggtgaa
     1021 cgcaatgtct ggaatttcct tctcgaccgc ggcgacgagc gtgatggtcg acgactgccc
     1081 cacgggcgcg cgagcggcgg tcacacaggt atgcgcggtg gccgggccgg cggtcgcgga
     1141 gatcgtggcg ccggccacca gaacgaacag cagcaagcgg cgcaccggtt cacgctagct
     1201 ctgtggcccc gaatacctgg ggtgggcacg actcggtacg ctcgacccgt gcgcgtgcga
     1261 atcctgattc cggttgttgt gctttccctg accgcgctcg gcgcgtgtgg cgggagcgac
     1321 aaaccgaagg ctgagtccga atcgacctca actgcgccct ctcccggtgg cacggcgcac
     1381 gtcgcgaaag cggcgaccga cgatccctcg gtgtcggcga agatggtctg ctccgacgaa
     1441 gcgcaggaag acatctataa gggtgcgacg ggcgtgaagc ccacgaaggt cacgaccccg
     1501 acgtgggaag accacgtcta cgcgtgcgat ttcgtctacc cgaacggcac catgcggctc
     1561 gagaccaaag aactcgccga cgccgacgcg acaaagaagt tcttcgacga gcaggcggag
     1621 aagctcggca agaaggagcc gctcaccggt atcggtgagg aaggtttctc gaccgacgac
     1681 ggtggtgcgg tcattcgcaa ggactacaaa gtcctgacgg tcgacgtgtc gaagctgccc
     1741 gcgcagttcg ggctgccgct cagcccgcgc tccgacgtcg cgatcaacac cgccttcacc
     1801 atcttccagt gctgggtggg cgcgtaggcg tcagacctcg acccggcggg ggaggcgttg
     1861 cgctcccgcg gcggcggcga gtgcgaacgc gatgcagatc gcgaacgcga tagcgagcgc
     1921 gccaccgcgg ctccagccca gttcgtcacc ggccgcgacg attgcgccgg cgacgccggt
     1981 accgagcgcg atgccgagcg tgtcgttgag ctgcagtgaa gcggtggccg tgccttcacc
     2041 accctcgggc gcctgggcga gcacgaccaa cgagatcggc gcgtacgcga gtcccatgcc
     2101 gaaaccggcc acgccccagg caacgaccgc ggccgcgatc ggtgccggga ccggcaacaa
     2161 cgcgacgacg atcatcagcg cgacgccg
//
LOCUS       DASUGA010000730         2262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1049415, whole genome shotgun sequence.
ACCESSION   DASUGA010000730 DASUGA010000000
VERSION     DASUGA010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049415"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VFX21_14430"
     CDS             complement(<1..757)
                     /locus_tag="VFX21_14430"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX5097219.1"
                     /translation="MTVLDKHARATTRGIVLVNPHSGPDETDLDDLRARFGEHDVVEC
                     EPADIATQTQASVDAGRPFVAVAGGDGTIRCAAEVLANTDVALLPIPAGTHNHFAKAL
                     GIATLDDAILAAKTGHRAAIDVARVNGKLFVNNSSLGTYPGVVAEREQHEGRMPKPLA
                     NVIAVFHQLRAGRRLTVDIDGRPRRAWLVFVGNGAYGENLVDITTRESLRDGVLDVRI
                     VRADQRFARLRLIVDALRGRLDQSPVLERRTCRA"
     gene            complement(879..1319)
                     /locus_tag="VFX21_14435"
     CDS             complement(879..1319)
                     /locus_tag="VFX21_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097220.1"
                     /translation="MSRPTLNHVAISMDPAILDTRGRQDLLAFFGEVFGWAEGDNTGE
                     PGNPLILYTGAFGQFVYLLPADPPLTAPPLDHFGLQVETLEELHEIVERAKAYAEKDG
                     RVEIIDVHARTTHGPAHDYVLTSAYIGYVLPLMIELQHLERVDR"
     gene            complement(1316..>2262)
                     /locus_tag="VFX21_14440"
     CDS             complement(1316..>2262)
                     /locus_tag="VFX21_14440"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX5097221.1"
                     /translation="GDALVAVSHCGICGTDLHLVLEQYARAGSILGHEWSGTIAAVGP
                     GVAGWEPGTPVVLNPVGGCGECRACRGGRPAVCLRREPPDLLDFSRGAFTRFKLVRAA
                     QLLRIPDELPLRAAALTEPTAIAIHTVNLSGVEAGDRVLITGGGPVGLLTTAVLRTRG
                     VDDITVSEPAPLRRERAQKVGATRVVEPADLPRAPMGRAVSEPYTFAFECSGNARAAE
                     SALDQLDYAGTLVFVGTGHESPRVNHNRMIVMEQTIIGAYNYDADGFVSALRLLASGE
                     LPLEHLIEPVDIGLSDVHTTMQRLAAGDLPGKVMVVPQ"
BASE COUNT          350 a          740 c          803 g          369 t
ORIGIN      
        1 tggcccggca cgtgcggcgt tcgagcaccg gcgactggtc gagccggcct cggagcgcgt
       61 cgacaatgag ccggaggcgc gcgaagcgtt gatcggcccg aacgatgcgt acgtcgagga
      121 cgccgtcgcg cagtgattcg cgcgtcgtga tgtcgacgag gttctcgccg tacgcgccgt
      181 tgccgacgaa caccaaccac gcccgacggg gacggccgtc gatgtcgacg gtcagccggc
      241 ggccggcgcg gagttgatgg aacactgcga tcacgttcgc gagcggcttc ggcatccgac
      301 cttcgtgctg ctcacgttct gcgacgaccc ccgggtaggt gccgaggctc gagttgttca
      361 cgaagagctt gccgttgacg cgcgcgacgt cgatcgccgc gcggtgtccg gtcttggctg
      421 cgagaatcgc gtcgtcgagc gttgcgatgc cgagcgcctt ggcaaagtga ttgtgggtgc
      481 cggccggaat gggcagcagc gcgacatcgg tgttggcgag cacctcggcc gcgcaccgga
      541 tcgtgccgtc gccgccggcc acggcaacga acggccggcc ggcgtcgacc gaggcttgcg
      601 tttgcgtcgc gatgtcggcg ggttcgcact cgacgacgtc gtgctcgccg aatcgcgcgc
      661 gtaggtcgtc gagatccgtc tcgtcggggc ccgaatgcgg gttcacgagc acgatcccgc
      721 gcgtcgtcgc gcgcgcatgc ttgtcgagaa cggtcattgc tatccccctg aggaagaaat
      781 tgatatgccc cgaatcgccc gcctgtcaat cctcgtcgga cacaaatgac caacttgcgt
      841 gacaaagggc ccctctaggg accgtttgtc acgccggttc agcggtcgac gcgctcgagg
      901 tgttgcagct cgatcatgag cggaagcaca taaccgatgt acgcgctcgt gagcacgtag
      961 tcgtgcgccg ggccgtgcgt cgtgcgcgcg tgcacgtcga tgatctcgac gcggccgtcc
     1021 ttctcggcat atgccttcgc gcgctcgacg atctcatgca gctcctcgag agtctctact
     1081 tgcagcccga agtggtccag cggcggtgcc gtcagcgggg gatcggcggg cagcaggtag
     1141 acgaactgcc cgaacgcgcc ggtgtagagg atgagcgggt tgccgggctc gccggtgttg
     1201 tcgccctcgg cccagccgaa aacctcaccg aagaatgcga gcaggtcctg acggccgcga
     1261 gtgtcgagaa tggcgggatc catgctgatc gcgacgtggt tgagtgtcgg gcggctcatt
     1321 gcgggaccac catcaccttc ccgggcaggt cgcccgcggc gagccgttgc atcgtcgtgt
     1381 gcacatcgct gaggccgatg tcgaccggtt cgatcagatg ctccagcgga agctcgcccg
     1441 acgccaggag ccggagcgct gacacaaatc cgtcggcgtc gtagttgtac gcaccgatga
     1501 tcgtctgttc catcacgatc atccggttgt ggttgacgcg cggggactcg tggcccgtgc
     1561 cgacgaagac gagcgtgccg gcatagtcga gctggtcgag cgcgctttcg gccgcgcgcg
     1621 cgttgcccga acactcgaac gcgaacgtgt acggctccga cacggcgcgc cccatcggtg
     1681 cgcgcggcag gtccgcgggc tcgacgacgc gcgtcgcgcc gaccttctgc gcgcgctcgc
     1741 gccgcagcgg cgccggttcc gacaccgtga tgtcgtcgac accgcgtgtg cgcagcaccg
     1801 cggtcgtgag caggccgacg ggtccgccgc cggtgatgag cacgcgatcg ccggcctcga
     1861 ccccgctcag gttcacggtg tggattgcga tcgcagtcgg ttcggtcagc gcggccgcgc
     1921 gcagcggtaa ttcgtcgggg atgcgcagaa gctgcgcggc gcgaacaagt ttgaagcgcg
     1981 tgaacgcgcc gcgcgagaag tcaaggaggt cgggcggctc gcgtcgcaga cagaccgcgg
     2041 gccggccgcc ccggcacgct cggcattcgc cgcagccgcc gacgggattg agaactaccg
     2101 gggtgcccgg ctcccagccc gcgacgccgg ggccgacggc ggcgatggtg cccgaccatt
     2161 cgtgcccgag tattgagccg gcgcgggcgt actgctcaag gacgaggtgc agatcggtcc
     2221 cgcagattcc acagtgcgat accgcgacga gcgcgtcgcc cg
//
LOCUS       DASUGA010000731         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_699891, whole genome shotgun sequence.
ACCESSION   DASUGA010000731 DASUGA010000000
VERSION     DASUGA010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699891"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VFX21_14445"
     CDS             <1..568
                     /locus_tag="VFX21_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097222.1"
                     /translation="VAGRRVPVIGSVQGLSPDRTIVGIAATADRTGYRLVANDGSVFP
                     FNAAARGEVTVHSAKDAAVGIASSTDDGYWIAQRDGSVGSFSAPRFDRATIPAGDYVV
                     GIASTPKGRGYWLLTAGGRVVGAGVRNHGDLTTMNVRAHATAIAAAPGGGYWITTDDG
                     RVYSFGSASAATSSAKQPLSQIVAVVPG"
     gene            complement(581..1153)
                     /locus_tag="VFX21_14450"
     CDS             complement(581..1153)
                     /locus_tag="VFX21_14450"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5097223.1"
                     /translation="MTSVGEERAATVALQLERMRPDTFAHSQRVATLAAAVAMHARLP
                     QELVTEVYWGALVHDIGEMSVVRSVLEKQGVLSEPERTAMGEHTIVGARWLAGIPGLA
                     PLVPFARWHHERFDGLGYPDGATAQQVPLTVALVGVCDAWDALTEDRPYRAALSVDEA
                     AAEMWRHAGRQWSRALVQWLLECVADTGVL"
     gene            complement(1259..2050)
                     /locus_tag="VFX21_14455"
     CDS             complement(1259..2050)
                     /locus_tag="VFX21_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5097224.1"
                     /translation="MTAAMLTVVGWNRRPWRLVERHALAYRRMWYIFLSGFAEPIFFL
                     LSIGVGVGELVGTLRIGDHVVDYRTFVAPGLLATTAMTGALLDTTFNFFAKMKYAHTY
                     DAVLATPLGTHDVAVGEITWALLRATAYSGAFVLTMWAFGDLHSPWAVLCLPAAVLIA
                     FAFAGAGLACTTFMRTFIDFDYVNMVMIPLFLFSATFFPIDRYPDWLEAIVRCTPLYQ
                     GVALERSLVVGDLNWTMPIHAVYLVAMGWIGLKVASKRIGKLLQP"
BASE COUNT          392 a          755 c          710 g          325 t
ORIGIN      
        1 cgtcgccggc cgccgcgtgc ccgtgatcgg gtcggtccaa ggtctgtcgc ccgatcgcac
       61 gatcgtgggc atcgccgcca ccgccgaccg caccggctat cgcctcgtcg cgaacgatgg
      121 cagcgtgttt ccgttcaatg ccgcggcacg gggcgaagtc accgtgcatt ccgcgaagga
      181 tgctgcggtc gggatcgcga gttcaaccga cgacggctac tggatcgcgc aacgcgacgg
      241 cagcgtcggt tctttctctg caccgcgatt cgacagagca acgatcccgg ccggcgacta
      301 tgtcgtcggc atcgcgtcga cgcccaaggg ccgcggctat tggctcttga ccgcaggcgg
      361 ccgggtcgtc ggcgcgggcg tacggaacca cggcgacctc accacgatga acgtgcgcgc
      421 ccacgccacc gcgatcgcgg ctgcgcccgg tggcggctac tggatcacaa ccgacgacgg
      481 gcgtgtgtac tcattcggga gtgcatccgc cgcgacgtcg tcggccaagc agccgctctc
      541 gcaaatcgtc gcggtcgttc cgggctgaat cgctacggtc ctacagcacg ccggtgtcgg
      601 cgacgcattc gagcagccac tgcacgaggg cgcgactcca ctgccggccc gcatggcgcc
      661 acatctccgc cgcggcttcg tcgacactga gcgcggcgcg gtacggacga tcttccgtga
      721 gcgcgtccca tgcgtcacac acaccgacga gggcgacggt gagcggcacc tgctgcgcgg
      781 tggcgccgtc cggataaccg agcccgtcga agcgttcgtg gtgccagcgc gcgaacggca
      841 cgagcggcgc cagcccgggg atgccggcga gccagcgcgc accgacgatc gtgtgctccc
      901 ccatcgccgt gcgttccggt tcgctgagca cgccctgctt ctccaagacg ctccgcacga
      961 ccgacatctc cccgatgtcg tgaacgagcg caccccagta gacctcggtg acaagctctt
     1021 gcggcagccg ggcgtgcatg gcaaccgccg cggccagcgt cgcgacgcgc tgcgagtgcg
     1081 caaacgtgtc gggacgcata cgctcgagct gcaacgcgac cgtcgcggcc cgctcctcgc
     1141 cgacgctcgt caaagcgctc cgactcgact ccatcccaga ggcatcgggc ccccgtctcg
     1201 gcggcttgaa cgatggagcg cttttcaggc gcggttttcg cacccgaaaa gcgcctcctc
     1261 acggctggag gagcttgccg attcgtttgg aggcgacttt caggccgatc cagcccatgg
     1321 caacgaggta gacggcgtga atgggcatcg tccagttcag gtcgccgacc accagcgacc
     1381 gctccagcgc aacgccctga tacagcggcg tgcagcgcac gatcgcttcg agccagtcgg
     1441 gataccggtc gatcgggaag aacgtcgccg agaacaagaa cagcgggatc atcaccatgt
     1501 tcacgtagtc gaagtcgatg aacgtgcgca tgaaggtggt gcacgccagt cccgcgccgg
     1561 caaacgcaaa ggcgatcaac actgcggccg gcagacacag caccgcccac ggcgaatgga
     1621 gatcgccgaa cgcccacatc gtgagcacga acgcgcccga atacgccgtc gcgcgcagca
     1681 acgcccacgt gatttcgccc accgcgacgt cgtgggtgcc gagtggcgtg gccagcaccg
     1741 cgtcgtaggt gtgtgcgtac ttcatcttgg cgaagaagtt gaacgtcgtg tcgagcagcg
     1801 cgccggtcat cgcggttgtc gccaacaatc cgggcgcgac gaacgtgcgg tagtcgacga
     1861 cgtgatcgcc gatgcgaagc gtgccgacga gttcgcccac tccgacgccg atcgacaata
     1921 agaagaagat cggctccgca aacccggaga ggaagatgta ccacatccgc cggtacgcga
     1981 gcgcgtgccg ttcgaccagc cgccacggcc ggcgattcca tccgacgacg gtgagcatcg
     2041 ccgcggtcat gcgttgagtc gcttctgata ggtgcgtacg ccgaagagcg cgccgatcgc
     2101 gatgcatgcg ctgagaaatg cgacatgacc gagcgacggc cagaactcga gcgtgccggt
     2161 cgtcgccccg cgcgccaact cg
//
LOCUS       DASUGA010000732         2622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_875799, whole genome shotgun sequence.
ACCESSION   DASUGA010000732 DASUGA010000000
VERSION     DASUGA010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875799"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VFX21_14460"
     CDS             <1..330
                     /locus_tag="VFX21_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006350888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX5097225.1"
                     /translation="MSDNLRPVTYGARYEAFLPARVRDARPEVVTVAGKHCEQGDLLV
                     RGATMPAGIAVGELLAIPVTGAYGYAMASNYNKVTRPAVVFVADGRARVVVRRERPED
                     LVRLDLI"
     gene            373..1662
                     /locus_tag="VFX21_14465"
     CDS             373..1662
                     /locus_tag="VFX21_14465"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX5097226.1"
                     /translation="MDDAVRVGILGCGNVGSALVRLLDDNAPLIVRRTGVRISVARVA
                     VHNMTKDRDVALAEGVLTHDAEAVVGNPDVDVVVEVIGGIEPARSLILAALKAGKPVV
                     TANKELLANFGEELFETAEASGVDLLFEASVAGGIPLMRPLRESLAGERIRRVMGIVN
                     GTTNYILSRMSEEGSSFADALAEAQRLGYAERDPTADIEGYDAAAKASIIASIAFGAR
                     VVAGDVYREGITQITGDDIAAARELGYVVKLLAVAESIDGTVGVRVHPAMVPLDHPLA
                     SVRGSYNAVFIEGDSVGQLMLLGRGAGGGPTASAVLGDLIDAAKNLRSGSRGATIGTL
                     HKTPIRPIDETASQFYLSLDAADQPGVLAAIAGVFGEHGVSIQSMQQQGAGSEARLIF
                     VTHLAREADMANTIHAVRDLDVVARVGSVLRVVGGEE"
     gene            1659..>2622
                     /gene="thrC"
                     /locus_tag="VFX21_14470"
     CDS             1659..>2622
                     /gene="thrC"
                     /locus_tag="VFX21_14470"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016824625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX5097227.1"
                     /translation="MSHFGWGGIIEAYRDRLPVNDKTPVVTLLEGNTPLVPAPRLSET
                     VGASVFLKVEGLNPTGSFKDRGMTVAMSKAAEEGAKVVVCASTGNTSASAAAYAARAG
                     MIAAVVIPDGHVALGKLAQALIHGARVVQIRGSFDDALDLVRTLGERGGVTVVNSINP
                     FRIEGQKTAAFEICDALGDAPDVHCIPVGNAGNITAYWKGYGEYADDGKAQRRPRMLG
                     WQAAGSAPLVHGEPVAHPETIATAIRIGNPASWDNAIAARDESGGAISAVTDAEILDA
                     YKLLASAEGVFVEPASAASVAGLLKGAQQGLVVRGETVVCTVTGH"
BASE COUNT          408 a          866 c          953 g          395 t
ORIGIN      
        1 atgagcgaca acctgcggcc ggtcacctat ggcgcgcgtt acgaagcgtt cctgcccgcg
       61 cgtgtgcgcg acgcacgacc cgaggtcgtg acggtcgcgg gcaagcactg cgagcagggc
      121 gacctgctcg tgcgcggcgc gacgatgccg gccggcatcg cggtcggcga gttgctcgcg
      181 atccccgtca ccggtgccta cgggtacgcg atggcgtcca actacaacaa ggtgacgcgc
      241 ccggcggtcg tgttcgtcgc cgacggccgg gcccgggtcg tcgtccgccg ggaacgcccc
      301 gaggacctgg tccgcctcga cctgatctga cacctgatct gggcacgccg cactttcggg
      361 caaaatggag aagtggacga cgccgtgcgg gttggcatct tgggctgcgg gaacgtcggg
      421 agcgcgttgg tgcgcctcct cgacgacaac gccccgctga tcgtgcggcg gacgggcgtc
      481 cgcatctcgg tggcccgggt cgcggtccac aacatgacca aggaccgcga cgtcgccctg
      541 gccgagggtg tgctcaccca cgacgcggaa gcggtggtcg gcaatcccga cgtcgacgtc
      601 gtcgtcgagg tcatcggcgg catcgaaccg gcgcggtcgc tgatcctcgc ggcgctgaag
      661 gccggcaaac cggtcgtcac cgcgaacaag gagctgctcg cgaacttcgg cgaggaactg
      721 ttcgagaccg ccgaagcatc gggcgtcgac ttgctgttcg aagcatcggt cgcgggcggc
      781 atcccgctga tgcgaccgct gcgcgagtca ctcgcgggcg agcgcatccg gcgggtgatg
      841 ggcatcgtca acggcacgac caactacatc ctgtcgcgca tgagcgaaga gggctcgtcg
      901 ttcgccgacg cgctcgccga agcgcaacgc ctcggttacg ccgagcgcga cccgacggcc
      961 gacatcgagg gttacgacgc cgcggcgaag gcgtcgatca tcgcgtcgat cgcgttcggg
     1021 gcgcgcgtcg tcgcgggcga cgtctaccgc gagggcatca cgcagatcac cggcgacgac
     1081 atcgcagcgg cgcgcgagct gggttacgtc gtcaagctgc tggcggtggc cgaatccatc
     1141 gacgggacgg tcggcgtgcg cgtgcacccg gcgatggtgc cgctcgacca cccgctcgcc
     1201 agcgtgcgcg gttcgtacaa cgcggtgttc atcgaaggcg actcggtcgg tcagctcatg
     1261 ctgctcgggc ggggtgccgg cggcggcccg acggccagtg cggtgctggg cgacctgatc
     1321 gacgcggcga agaacctgcg gtcggggtcc cggggcgcca ccatcggaac actgcacaag
     1381 acgccaatcc ggccgatcga cgagacggcg tcccagttct acttgtcgct ggacgcggcc
     1441 gatcagcccg gtgtgctggc ggccattgcc ggagtgttcg gggagcacgg cgtgtcgatc
     1501 cagtccatgc agcagcaagg ggcggggagt gaagctcgtc tcatcttcgt gactcacctt
     1561 gcgcgcgagg ccgacatggc caacacaatt catgcggtgc gcgacctcga cgtcgtcgcg
     1621 cgagtcggat ccgtcctgcg ggtcgttggc ggtgaagagt gagccacttt ggctggggcg
     1681 ggatcatcga ggcgtaccgc gaccggctgc cggtcaacga caagacgccg gtcgtcacct
     1741 tgctcgaggg caatacgccg ctcgtgcccg cgccgcggct gtccgagacc gtcggcgcct
     1801 ctgtcttctt gaaggtggag ggtctcaacc cgaccggttc gttcaaggac cgcggcatga
     1861 cggtcgcgat gtcgaaggcg gccgaggagg gcgcgaaggt cgtcgtctgc gcgtcgacgg
     1921 gcaacacgtc cgcatccgcg gccgcgtacg cggcgcgcgc ggggatgatc gccgcggtcg
     1981 tcatccccga cggccacgtg gcgctgggga agctcgcgca ggcgctcatc cacggtgcgc
     2041 gcgtcgtgca gatccgcggc agcttcgacg acgcgctcga tctcgtccgc acactcggtg
     2101 aacgcggtgg tgtcacggtc gtgaactcca tcaacccgtt ccgcatcgag gggcagaaga
     2161 cggcggcgtt cgagatctgc gacgcgctcg gtgacgcgcc cgacgttcac tgcatccccg
     2221 tcgggaacgc gggcaacatc acggcctact ggaagggcta cggcgagtac gcggacgacg
     2281 gcaaggcgca acgccggccg cgcatgctcg gctggcaggc cgccggatcg gcgccgctcg
     2341 tgcacggtga gccggtcgcg catcccgaga cgatcgcgac cgcgatccgg atcgggaacc
     2401 ctgcgagctg ggacaacgcg atcgcggccc gcgacgaatc gggcggcgcg atcagtgcgg
     2461 tcaccgatgc cgagatcctc gacgcctaca agctgttggc ctcggccgaa ggcgtgttcg
     2521 tcgagcccgc atcggcggca tcggtcgcgg gattgttgaa gggcgcgcag caggggctcg
     2581 tcgtgcgcgg cgaaactgtg gtttgcacgg tgaccggaca tg
//
LOCUS       DASUGA010000733         5311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_319570, whole genome shotgun sequence.
ACCESSION   DASUGA010000733 DASUGA010000000
VERSION     DASUGA010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319570"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VFX21_14475"
     CDS             complement(<1..508)
                     /locus_tag="VFX21_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097228.1"
                     /translation="MMVAVHQFVPTLAPRDAVGAHSIAVQSVLRDAGYRSDIFVLESR
                     GRYRKGVRDYHDFDGGRPGEPTWIFFQMSVGSPVCDFVVARNEPLIVNYHNITPTEYF
                     APWEPIVAANLSRGRRQLRSLARRADVGIAVSEFNEKDLITAGFGATDVAPVLVDSTD
                     WDIEPDRRV"
     gene            complement(505..1566)
                     /locus_tag="VFX21_14480"
     CDS             complement(505..1566)
                     /locus_tag="VFX21_14480"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5097229.1"
                     /translation="MRIHAARYLDNGLGFNHGQPATLIEIERFTDYDDLLAYIVDSNF
                     YGDAYLRRQSGQTDDRLIAHFWWLADTVAALQPHRVLEIGCGRGDVLRLLHEAHGIEV
                     AGIDFGSSVSDRMWPSVRANFHAGEIREVLQTWSGPAYDLAGGFDIWEHLHPRDLDET
                     IELLVEHSTRDALFVFVIPAFGTDEVFGEQFPLEFEENRDAFEHREPFRFLLSDPSDE
                     KVPAAGHLTWAHTDWWVARFVAKGLVREPRVERALHPIIDPHVPHSIKSFYVFRRDTP
                     EARARIEHWHGPRVKAAAGLQSLARREYFRRRRHAEFNVGTRVEFGRWWRAQGPVIQG
                     AARTAGWTKRQLSRLTPGK"
     gene            1647..3488
                     /locus_tag="VFX21_14485"
     CDS             1647..3488
                     /locus_tag="VFX21_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097230.1"
                     /translation="MSLRVDARNRRALDADDRPEVLFGDHAVPPRDRAATVRVVLAYI
                     VIIAVMTFPTYPSRLSTFIPGDSGDALLNLWILNWAGHHVGGWHELWNTSIFYPHTNT
                     LAYSESMLPVALAHRAIALVVRSDVAAFNLLYLAGQFLSAWCTYRLARRMHAGVAGAF
                     LAGLVYTLSTPRFAHYQHFQLVFGWLVPLVVLLVVRYFESPTVRRGAALGAVSGVLAL
                     CASYYGLMTFVAVGIISVALLLWGSPLPRRRVIAGLAVAAGCAMLIVVPVARQYTLLQ
                     RDDHFRREPEAALAAHLADFTRVTPDNYLLAEYGPLASRSEPQDGTIENRLFPGSLAL
                     GLGLVGLGAIVRRRAAAFGNDPLAFRAFVGTGIAALTLLILSFGTRLEVAGHSLWMPY
                     SLLRDVPGFSGIRATSRLAAFAMLALALCAAIGCGVLVRGRSTLVRGAVVTALTALLL
                     AESATSIVFTRVPDAGAARAVNEALADRPPGPVAELPVGSPADGAAWGYIETPRQWLS
                     RIDGDPRVSGYSGFDPPGFDAVASVLDTFPSEEAFTVLQSLGVRYVVIRTALPGPLRG
                     FQEDYVALDGVGFIAPERARGVIETVRNDPRVRRVDHYGDAWLIELK"
     gene            3592..4656
                     /locus_tag="VFX21_14490"
     CDS             3592..4656
                     /locus_tag="VFX21_14490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5097231.1"
                     /translation="MLVHEYCSDLEPGAVGAHALVVRDLLQQAGHRCEIVTPRLDPAY
                     AASGAHLLGEVRGTPDLLLYQMGIGAEVADVLLARTEPLVVNYHNLTPSRLLAGWDPV
                     AAHGVVWGRQQLRALAPRSESGIAVSRYNEAELVEAGFAHTAVVPFLLDLAAFGAEPD
                     AALLERLRATRAGTEWLFVGRVAPHKAQHDLLKAFASYRRFHDADARLHLVGGGLDSA
                     YGRALTNFASSLGLGDSVVLTGAVAGGALAAYYANADVFTVASEHEGFCVPLLESWYH
                     RVPVVAYAAAAIPETLGDGGLLLDAKDPYTFAAAVARVVPGGPVRETLVANGSARLAQ
                     FDLAVTGPAFVETLTGVTAR"
     gene            4653..>5311
                     /locus_tag="VFX21_14495"
     CDS             4653..>5311
                     /locus_tag="VFX21_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097232.1"
                     /translation="MKLAVVTPRYGTDIGGGAETAARELSTRLAQHPDWDVEVLTTCA
                     RDAISWANEMPPGTTTEDGVTVHRFPTTRTRPGDFDATSARVVHQRPRPPVEAQRAWI
                     EAQGPYAPALVDAIRATDADAVAFHPYLYYPTVAGVPEVADRAVMHPAAHDEAAIRLP
                     IFRDVFGAAAAFVYWTDTERRFAERLFRVAARPQLVLGLGTAPGPGTPDAAREQCGLG
                     DR"
BASE COUNT          742 a         1907 c         1799 g          863 t
ORIGIN      
        1 tgacgcgccg gtcgggttcg atgtcccagt ccgtcgagtc gacgagcacg ggcgcgacat
       61 cggtcgcacc gaatccggcg gtgatgaggt ccttttcgtt gaactcggag accgcgatcc
      121 cgacgtccgc gcgtcgcgcc agcgaacgca gctgacgccg gccgcgtgac aagttcgcgg
      181 caacgatcgg ttcccaaggc gcgaagtact cggtcggcgt gatgttgtgg tagttgacga
      241 tcagcggctc attgcgagcg acgacgaagt cgcacacggg agatcccacc gacatctgga
      301 agaagatcca cgtcggttcg cccgggcggc cgccgtcgaa gtcgtggtag tcgcgcacgc
      361 ccttgcggta acggccacga gactcgagca cgaagatgtc ggagcggtat ccggcgtcgc
      421 gcagcaccga ctgcaccgcg atgctgtgcg ccccgaccgc gtcgcgcggt gcgagcgtcg
      481 ggacgaactg gtggaccgcg accatcactt ccccggtgtc aaccggctga gctgccgttt
      541 ggtccagccc gcggtgcgcg ccgcgccctg gatgaccggg ccctgggccc gccaccagcg
      601 cccgaactcg acgcgcgtcc cgacgttgaa ctcggcgtgc cggcggcgcc ggaagtactc
      661 gcgccgggcg agcgactgca gcccggcggc agccttcacg cgcggcccgt gccagtgctc
      721 gatacgagcg cgcgcctcag gggtgtcccg ccggaacacg tagaacgact tgatcgaatg
      781 cggcacgtgc ggatcgatga tcggatgcaa cgcgcgttcg acgcgaggtt cgcgcacgag
      841 tcccttggcg acgaagcgcg ccacccacca gtcggtgtgc gcccacgtga ggtggccggc
      901 cgccggcacc ttctcgtcgc tcgggtccga gagcaagaac cggaacggct cgcggtgctc
      961 gaacgcgtcg cggttctcct cgaattcgag cgggaactgc tcaccgaaga cctcgtcggt
     1021 gccgaacgcg gggatgacga agacgaacag cgcgtcgcgc gtgctgtgct cgacgagcaa
     1081 ctcgatcgtc tcgtcgagat cgcgcgggtg gaggtgctcc cagatgtcga acccgcccgc
     1141 gaggtcgtac gcgggcccgg accacgtctg gagcacttcg cggatctcgc ccgcgtggaa
     1201 gttggcgcgc accgacggcc acatgcggtc ggagaccgaa ctgccgaagt cgatgcccgc
     1261 gacctcgatg ccgtgcgctt cgtgcagcag gcgcaagaca tcgccgcggc cgcacccgat
     1321 ctccagcacc cggtgcggct gcagcgccgc gaccgtgtcg gcgagccacc aaaagtgcgc
     1381 gatcaggcgg tcgtcggtct gtccggactg ccgccgcagg tacgcgtcac cgtagaagtt
     1441 cgagtcgacg atgtacgcga gcaggtcgtc gtagtcggtg aagcgttcga tctcgatcaa
     1501 cgtcgcgggc tggccgtgat tgaagccgag tccattgtcg agatagcgcg cggcgtggat
     1561 tctcatagcg cctccatcgc cgaaccacgc taacagtcga ccgcgtcggg tccaggactg
     1621 tgtgcacagc gcggtagcct gctccggtgt cgctgagggt tgacgctcgc aaccgacgcg
     1681 ccctcgacgc ggacgaccga cccgaggttc tgttcggcga ccatgcagtg ccgccgcgcg
     1741 atcgcgccgc gaccgtgcgt gtcgtcctcg cgtacatcgt gatcatcgcg gtaatgacgt
     1801 tcccgacgta cccgtcgcgg ttgtcgacgt tcatccccgg cgacagcggt gacgcgctgc
     1861 tgaacctgtg gatcctcaac tgggccggcc atcacgtcgg cggttggcac gagctgtgga
     1921 acacgtcgat cttctatccg cacaccaaca cgcttgcgta cagcgagtcg atgctgcccg
     1981 tggcgctggc ccatcgcgcg attgcgctcg tcgtgcgctc cgacgtcgcg gcgttcaacc
     2041 tcttgtacct cgccggccag ttcctgtccg cgtggtgcac gtaccggctg gcccgccgta
     2101 tgcacgcggg cgtcgccggc gccttcctcg cggggctcgt gtacacactc tcgacgccgc
     2161 gcttcgcgca ctatcagcac tttcagctcg tgttcggttg gctggtcccg ctcgtcgtgt
     2221 tgctcgtggt ccggtacttc gagtcaccga cggtgcgccg cggtgccgcg ctgggtgccg
     2281 taagcggcgt gctcgcgttg tgcgcgagct actacggact gatgacgttc gtcgcggtcg
     2341 gcatcatctc agtggcgctg ttgttgtggg gctcgccgct gccccgccgg cgcgtcattg
     2401 cagggctcgc ggtcgccgcc ggttgcgcca tgttgatcgt cgtccccgtc gcgcggcaat
     2461 acacgctgtt gcagcgcgac gaccactttc gccgcgaacc cgaagcggcg ctcgccgcgc
     2521 atctcgccga cttcacgcgc gtcacacctg acaactacct cctcgccgaa tacggcccgc
     2581 tcgcgtcgcg ctccgagccg caagacggca cgatcgagaa ccggttgttc cccggctccc
     2641 tcgcgctcgg gctcggcctg gtcgggctcg gcgcgatagt gcgccgccgc gcggcggcgt
     2701 tcggcaacga tcccctggcg tttcgcgcgt tcgtcggcac cggcatcgcg gcattgacgt
     2761 tgctgatcct ctccttcggg acgcgtctcg aggtcgccgg ccactcgctc tggatgccgt
     2821 actcgctgct ccgggacgtc ccgggcttct cgggcatccg cgcgacgtcc cggcttgcgg
     2881 cgttcgcgat gttggcactc gcgctgtgtg ccgcgatcgg gtgcggcgtc ctcgttcggg
     2941 gccggtcgac acttgtacgc ggggccgtcg tcaccgcgct cacggcgctt ctcctcgccg
     3001 agagcgcgac ctcgatcgtg ttcacgcgcg tgcccgacgc cggtgccgcg cgggccgtca
     3061 acgaagcact cgccgaccga ccgccgggcc ctgtcgccga actgccggtc ggctcgccgg
     3121 ccgacggtgc ggcgtgggga tacatcgaga cgccacgcca gtggctgtcg cgcatcgacg
     3181 gtgacccacg agtgagcggc tacagcggct tcgacccgcc cggcttcgac gccgtcgcgt
     3241 cggtgctcga cacgttcccg agcgaagaag cgttcaccgt gctgcagagt ctcggcgtac
     3301 ggtacgtcgt catccgtact gcgctgcccg gcccgctccg cggcttccaa gaggactacg
     3361 tcgcgctcga cggcgtcggg ttcatcgcgc cggaacgcgc ccggggcgtc atcgagaccg
     3421 tccggaacga tccgcgcgtc cgccgcgtcg accactacgg cgacgcgtgg ctcatcgagc
     3481 tcaagtagcc gtcacgagcg agaccgtcgt gatggcgcga cgtgtcaagg cgacaaatct
     3541 cgaggatgca ggcgccggga tcgcgtcatc gaccgcacta tcctcggcga cttgctcgtc
     3601 cacgagtatt gctccgactt ggaaccgggc gccgtcggtg cgcacgcgct cgtcgtgcgc
     3661 gatctgttgc aacaagccgg gcaccgctgc gagatcgtta ccccgcgctt ggacccggcc
     3721 tatgcggcca gcggcgcaca tctgctcggc gaggtgcgcg gcacgccgga tctcctcttg
     3781 taccagatgg ggatcggcgc cgaggtcgcc gacgtattgc ttgcgcggac cgagcccctc
     3841 gtcgtcaact accacaacct cacaccgtcc cggctgctcg cgggctggga tccggtcgcc
     3901 gcgcacggcg tcgtgtgggg gcgccagcag ctgcgcgcgt tggcgccccg gtcggagtca
     3961 ggtatcgcgg tctcacgcta caacgaagcc gagctggtcg aggccgggtt cgcgcatacg
     4021 gccgttgtgc cgttcctgct cgacctcgcc gcgttcggcg cggagcccga cgcggccctg
     4081 ctcgaacgcc tgcgcgcgac gcgcgccggc accgagtggc tgttcgtcgg ccgcgttgcg
     4141 ccccacaagg cgcaacacga tctcctgaag gcgttcgcgt cctaccgccg cttccacgac
     4201 gccgacgcgc gcttgcacct cgtcggcggc gggctcgaca gcgcgtacgg acgcgcgctc
     4261 accaacttcg cgtcgtcact cggtctcggt gactcggtcg tcctgaccgg tgcggtcgcc
     4321 ggcggcgcgc tcgccgccta ctacgcgaac gcggatgtgt tcactgtggc cagcgagcac
     4381 gaagggttct gcgtcccgtt gctcgagtcg tggtaccacc gcgtgcccgt cgtcgcgtac
     4441 gcggccgctg cgatccccga gacgctcggc gacggcggct tgctgctcga cgccaaggat
     4501 ccctacacct tcgcggccgc cgtcgcgcgc gtcgtccccg gtggacccgt gcgcgagacg
     4561 ctcgtggcca acggttcggc gcgcctcgcg cagttcgacc tcgcggtgac cgggcccgcg
     4621 ttcgtcgaga cgctgacggg ggtgaccgcc cgatgaagct cgccgtcgtc acgccgcgct
     4681 acggcaccga catcggtggc ggcgcggaga cggccgcgcg tgagctgtcg acgcgactcg
     4741 cacagcaccc cgactgggac gtcgaagtcc tgacgacgtg cgctcgcgac gccatctcgt
     4801 gggcgaacga gatgccgccc ggcacgacga cagaggacgg cgtcaccgtc caccggttcc
     4861 cgacgacgcg cacccgaccc ggcgacttcg acgcgacgag cgcccgcgtc gtgcaccaac
     4921 gcccgcgccc gccggtcgaa gcacagcgcg cgtggatcga ggcgcaagga ccgtacgcgc
     4981 cggccctcgt cgacgcgatc cgcgccaccg acgccgacgc ggtcgcgttc catccgtacc
     5041 tctactaccc gacggtcgcg ggcgtgcccg aggtggccga ccgggccgtg atgcacccgg
     5101 ccgcgcacga cgaggccgcg atccgcctgc cgatctttcg cgatgtcttc ggcgcagccg
     5161 ccgcgttcgt gtactggacc gacaccgaac gacggttcgc ggagcgactg ttcagggtcg
     5221 cggcgcgacc gcagctcgtg ctcgggctcg gcaccgcacc cgggccgggc acaccggacg
     5281 ccgcgcgcga acagtgcggc ctcggcgacc g
//
LOCUS       DASUGA010000734         4150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_217905, whole genome shotgun sequence.
ACCESSION   DASUGA010000734 DASUGA010000000
VERSION     DASUGA010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217905"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..948)
                     /locus_tag="VFX21_14500"
     CDS             complement(97..948)
                     /locus_tag="VFX21_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX5097233.1"
                     /translation="MALAAPPTLSPSSMGTFTSCPLSFRFSYLERLPEPPSAPASKGT
                     LVHLALQHLMWRPPTERTLAHALADLDRATAEVAAHPEFAHLELSDEQWAEFHADAAV
                     LVHRYFDLEDPAKINVLGVELRVSAVTEEGVTIRGIIDRLELDEHGDLVVTDYKTGSA
                     PSEGWEQKSLAGVHVYALLCERMFGRRPARVQLLYLSGPERIVTQPTDPSVRGVEVKT
                     NAVMRAVRTACARDDFRPRPSALCSFCSFREFCPSFGGDPALAAEVMLARESERQGRP
                     QLPFATV"
     gene            1077..2075
                     /locus_tag="VFX21_14505"
     CDS             1077..2075
                     /locus_tag="VFX21_14505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX5097234.1"
                     /translation="MAPTLAEVGARARELEEFGYDGLMTAETSSDPFLPLAIAAEHTE
                     RIELATGIAVAFARTPMVTAQVSNDLQRASNGRFILGLGSQIKPHIEKRFSMPWSHPA
                     ARMREYILALRAIWATWNEGAKLDFRGDFYQHTLMTPFFSPPPNPFGAPKVFLAAVGE
                     KMTEVAGEVADGIIIHGFTTERYVKEVTLPAIERGLARAGRDRSTFEISGPLFVVTGT
                     NDDEIENAKRGTRQQIAFYGSTPAYRGVLELHGWGDLQSELNSLSKRGEWVKMGELID
                     DEILDTFAVVAEPEAIGAELARRYAGVVDRCSFYAPYRSEPDRWRGVVESLKRVDA"
     gene            2288..3217
                     /locus_tag="VFX21_14510"
     CDS             2288..3217
                     /locus_tag="VFX21_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEX5097235.1"
                     /translation="MGSYNEQMLAIAGRQHQVVSRAQLLDIGTRRQLEHRLQSGMLER
                     VYDSVYRLKGSPETWRQKLMAATFAGGKLSVASFRAAAALHYLPGGKEIMEITSSRHR
                     RARHVGVYPHESKFLTDLDVMYIDNVPVTRTARTINDLGWLVEQSRMKPQVLDHAMHD
                     AVRRSIVDVERVVREWERLGGTFRAGGKAVETMLRNFVPPKRNPDSSAEIVLLQLLRD
                     AGFPDPVPQWRVWISPTKWYDLDFAWPWLKRFAEFNSYKYHGNWDKHERDALRLLLIR
                     DLGWDGVTVTDTELDKGAPNALRVLRKMLRRAS"
     gene            complement(3240..>4150)
                     /locus_tag="VFX21_14515"
     CDS             complement(3240..>4150)
                     /locus_tag="VFX21_14515"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5097236.1"
                     /translation="QRDHEGDRRPRRRTTTDVTPASHRFDAGSWSLAVYDWGGDGPPV
                     LLAHATGFHGRIWAPVVAELLARGRRVWSFDFHGHGDSDAPDIDYSWHGFADDVLAVA
                     RHVGVHGDPSLIAAGHSKGGASLFLGAVKDPDAFARIWAFEPIVFRWDRDITPDDVPL
                     AVSARKRRAIFASRDEAYERYASRPPLAAMTPESLRAYVDYGFRDLPDSTVELKCAPE
                     VEARVFSMGPQHGVYERLGEVTVPVRVVCGETSQSITPALGEIIAQRLPRGTFEMQPG
                     AGHFGPQQDPAWTAESILHTGVDSHR"
BASE COUNT          679 a         1362 c         1467 g          642 t
ORIGIN      
        1 acacgacgcg atcgagagca ggggagcgaa tgcggtcgaa cacggcgtcg accgcgccat
       61 caaattgcgc gagggcgaac cgcgaaccgg tcgcgctcac acggtggcga agggcagctg
      121 cggccggcct tggcgctcgg actcgcgggc cagcatcact tccgcggcca gcgccggatc
      181 gcccccgaac gacgggcaga actcacggaa cgaacagaac gagcacagcg ccgacggccg
      241 cggccggaag tcgtcgcggg cgcacgcggt gcggaccgcg cgcatgactg cgttcgtctt
      301 cacttcgacg ccgcgcaccg acgggtcggt cggctgcgtc acgatgcgct cgggccctga
      361 caggtagagc agctgcaccc gtgcgggacg ccgcccgaac atgcgctcgc acaacagcgc
      421 gtacacgtgc acgcccgcga ggctcttctg ctcccagccc tcgctcgggg cggagccggt
      481 cttgtagtcg gtgacgacga ggtcaccgtg ctcgtcgagc tcgaggcggt cgatgatccc
      541 gcggatcgtc acgccttctt cggtgactgc cgagacccgg agctcgacgc cgagcacgtt
      601 gatcttcgcc gggtcctcga ggtcgaagta gcggtggacg agcacggccg cgtcggcgtg
      661 gaactcggcc cattgctcgt cgctgagctc gaggtgggcg aactccggat gggccgcgac
      721 ctcggccgtc gcccggtcga ggtcggcgag cgcgtgggcg agcgtgcgct cggtgggcgg
      781 ccgccacatg aggtgctgga gcgccaggtg gacgagcgtg cccttgctgg ccggcgccga
      841 cggtggctcc ggcagccgct ccaggtagga gaaacggaac gagagcgggc agctggtgaa
      901 cgtccccatc gagctcgggg agagcgtcgg aggcgccgcc aatgccatgc gaggaccata
      961 ccgccggggt gcgacaacgg aggtggatgg cttagcgtcg ccgtgcggac acagcggagc
     1021 cacggacggc gcagcgaggg agtcatagat atctcgtgaa ggtggatacc ggagtcatgg
     1081 ccccgacgct cgccgaggtc ggcgcccgcg cgcgtgagct ggaggagttc ggatacgacg
     1141 gcctgatgac ggccgagacg tcgtccgacc cgttcctgcc gctcgcgatc gccgccgagc
     1201 acaccgagcg catcgagctc gcgaccggca tcgccgtcgc gttcgcgcgc acgccgatgg
     1261 tcaccgcgca ggtgtccaac gacttgcagc gcgcgtcgaa cggtcgcttc atcctcgggc
     1321 tcggctcgca gatcaagccg cacatcgaga agcggttctc gatgccgtgg tcgcacccgg
     1381 ccgcgcgcat gcgcgagtac atcctggcgt tgcgcgcgat ctgggcgacg tggaacgaag
     1441 gcgcgaagct cgacttccgc ggcgacttct atcaacacac gctgatgacg ccgttcttct
     1501 cgccgccgcc gaacccgttc ggagcaccga aggtcttcct cgccgcggtg ggggagaaga
     1561 tgaccgaagt cgcgggcgag gtcgccgacg gcatcatcat ccacgggttc accaccgagc
     1621 gatacgtgaa ggaggtcacg ctgcccgcga tcgaacgcgg gttggcgcgt gccggacgcg
     1681 atcgttcgac gttcgagatc tcgggaccgt tgttcgtcgt gaccggtacc aacgacgacg
     1741 agatcgagaa cgcgaagcgc ggcacgcgcc agcagatcgc gttctacggg tcgacacccg
     1801 cgtaccgcgg cgtgctcgag ctgcacggct ggggtgacct ccagtccgag ctcaacagcc
     1861 tctcgaagcg gggcgagtgg gtgaagatgg gcgagctgat cgacgacgag atcctcgaca
     1921 ccttcgccgt ggtcgccgag cccgaggcca tcggtgccga gctggcccgc cgctacgccg
     1981 gcgtcgtcga ccggtgctcg ttctacgccc cctaccgctc cgagcccgac cgctggcgcg
     2041 gcgtcgtgga gtcgctcaag cgagtggacg cgtagccggg gtcgttcgcc gaaaatcccg
     2101 ttcgtcaacc gggccggggg ccggcgcggt gcgaacccct cgaccccgcg gccgccggcg
     2161 gccgcgactt cgtcctctgg gtctcaccga acgctcccta acccgggcgt cgttcaacca
     2221 cacttgttgg gggcgaacga cgcccgtgtt cgcgttgctg tctcacctcg cggcgagcat
     2281 ggcggacgtg gggtcataca acgaacagat gctggcaatc gcgggtcgtc agcatcaggt
     2341 agtcagccgt gcgcaactcc tcgacatcgg gactcgaagg cagctcgagc atcggctgca
     2401 gagcggcatg ctcgaacggg tctacgacag cgtgtaccgg ctcaagggtt cacccgagac
     2461 gtggcggcag aagctgatgg ccgcgacgtt tgcgggcgga aagctgagcg tcgcatcctt
     2521 tcgggcggcg gctgctcttc actatctgcc gggcggcaag gagatcatgg agatcacctc
     2581 gtctcggcac cggcgggctc ggcacgtcgg cgtctacccg cacgagagca agtttctgac
     2641 cgacctcgac gttatgtaca tcgacaacgt cccggtcacg cgaacagcgc gcacgatcaa
     2701 cgacctcggt tggctggtgg agcagtcgcg gatgaaaccg caagtgctgg atcacgcgat
     2761 gcacgacgcg gtgcggcggt cgatcgtcga cgtcgagcgc gtcgtgcgag agtgggaacg
     2821 gctgggcggc acgttccgtg cgggtggcaa ggccgtagag acgatgctgc ggaacttcgt
     2881 gccgccgaaa cggaatccgg acagttccgc tgagatcgtc ttgctgcaac ttcttcgaga
     2941 tgcggggttt cctgatcccg ttccgcagtg gcgcgtgtgg atatcgccga caaagtggta
     3001 cgacctcgac ttcgcgtggc cttggctcaa gcggttcgcc gagttcaact cgtacaagta
     3061 ccacggcaac tgggacaagc atgagcgaga cgcgctgcgt ctcttgctga ttcgcgatct
     3121 cggttgggac ggcgtcaccg tcacggacac ggagctcgac aagggcgcac cgaacgctct
     3181 tcgggttctc cggaagatgc tgcgccgagc gagctgactc gggcgtcgct gaaccacaat
     3241 taccggtggc tatcgacgcc cgtgtgcagg atcgattcgg cggtccacgc cgggtcttgc
     3301 tgggggccga agtggcccgc gccgggttgc atctcgaagg tgcctcgggg gaggcgttgg
     3361 gcaatgatct cgccgagcgc gggcgtgatg ctctggctgg tctcgccgca cacgacccgc
     3421 acgggcaccg tcacctcgcc gagtcgctcg tacacgccgt gctgcgggcc catcgagaac
     3481 acgcgcgctt cgacctcggg cgcgcacttg agctcgacgg tgctgtcggg caggtcgcgg
     3541 aagccgtagt cgacgtacgc gcgcaacgac tcgggtgtca tcgccgccag tggcgggcgt
     3601 gacgcgtagc gctcgtacgc ctcgtcgcgg gacgcgaaga tcgcgcgacg cttccgcgcg
     3661 ctgactgcga gcggcacgtc gtcgggcgtg atgtcgcggt cccaacggaa gacgatcggc
     3721 tcgaacgccc agatgcgtgc gaacgcgtcc gggtccttca ccgcgccgag gaagagtgac
     3781 gcgccgcctt tggagtgtcc ggcggcgatg agtgacgggt cgccgtgcac gccgacgtgg
     3841 cgcgcgaccg cgagcacgtc gtcggcgaat ccgtgccacg aatagtcgat gtcgggtgcg
     3901 tcgctgtcgc cgtggccgtg gaagtcgaac gaccacacgc gccgcccgcg cgccaagagc
     3961 tcggcgacca ccggcgccca gatgcggccg tggaatcccg tcgcgtgcgc gagcagcacg
     4021 ggcggaccgt cgccgcccca gtcatacacc gccaacgacc acgacccggc gtcgaaccgg
     4081 tgcgacgccg gtgtcacgtc ggttgttgtt cggcggcgtg gtcggcgatc gccttcatga
     4141 tcgcgttggc
//
LOCUS       DASUGA010000735         3857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1050963, whole genome shotgun sequence.
ACCESSION   DASUGA010000735 DASUGA010000000
VERSION     DASUGA010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050963"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1228)
                     /locus_tag="VFX21_14520"
     CDS             complement(77..1228)
                     /locus_tag="VFX21_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX5097237.1"
                     /translation="MSGPLSGIRVLEIPNIGPLQHAGMILSDLGADVLRLDRAAHVRA
                     GTGLQGPVGPFSVLDRGRRSAGVDLKDPGGAEVVLRCCEHADVMVEGFRPGVAERLGI
                     GPDACLARNPRLVYGRMTGWGQDGPLAGDVGHDINYISLSGVLSHIGAAGGPPIPPIN
                     LIGDFGGGSMLLVIGVLAALVEGGRSGAGQVVDAAMVDGSALLMSIFFAIDQMGAWGP
                     RGTNLLDGGAHWYGVYETADGGYVSVAAFEPQFYVEFLRVMELSDLDPAQQMDKDQWP
                     ALKERVAQLFKAKTRDEWVEVMRGHEVCFAPVLTMAEARAHQHNVHRRTFVEVDGVAQ
                     PAPAPRFSRTPPDVPKAPAPTGSATDSALHDWGFSTEEILKLKTAGTVA"
     gene            complement(1241..2128)
                     /locus_tag="VFX21_14525"
     CDS             complement(1241..2128)
                     /locus_tag="VFX21_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5097238.1"
                     /translation="MSAEAFEAARASVGVIDTMIGFPAPREEQAKHYDFIRAQTHDSD
                     SQEWEFPAQYMFKGVPHYDRVDHPVEMLVAKMDELGIRKAITGLPTGGNSKEALERYP
                     DRFFPSCNVDPNQGMEAVRALELAVKEYGAKCAQCWGTGLIPQVPLDDKKMYPIYAKC
                     VELDIPIFVYAGVPGPRIRFAPQYVGLLDEVCWFFPELKIVTRHGGQPWTELMVKLLL
                     KWPNLYYSTSAFAPKHYDEHIIRFANTRGADKVMYAGYYPMGLDIDRIFAELPHVPFR
                     DHVWPKFLYENAARVLHLD"
     gene            complement(2125..3027)
                     /locus_tag="VFX21_14530"
     CDS             complement(2125..3027)
                     /locus_tag="VFX21_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5097239.1"
                     /translation="MNAAFDAAMASGGAVDTALGFPKSREDMYEFYGFIRKASLDKAT
                     KEGDLEFPAGYMFHNPPKWEQDEIDDPLMIVLAEMEQHGLSQVVVGVEDEVGRRAVRD
                     MPDRFFPAISIDPNEGMEAVRKIDRYADEFELRAVHAFPAGLYPPVALNDKMFYPIYA
                     KCVERDIAFCSTAGVPGPRLRFEPQDVAHIDEICWFFPELKFVTRHGCEPWTALAVKL
                     MLKWPNLYYSTTAFAPKYYPKDIVDYANTRGADKIIWSGYFPAGLTYDRIFGELPNVP
                     FRDHVWPKFLRENAQKVFKLPTTS"
     gene            complement(3024..>3857)
                     /locus_tag="VFX21_14535"
     CDS             complement(3024..>3857)
                     /locus_tag="VFX21_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5097240.1"
                     /translation="NPAPTGEQAEKYQKYLAQLRDRESKEDFTFPAQYMFKNVPEFAD
                     LDDPVAVLLAEMDTYGIRVGMLNVATNLEARRAVAEHPDRFIASWDVDPNRGMDAVRE
                     VEQAVEQLGVKSVQVFPAGLTPQVPINDKKMYPLYSKCVELDIPIFVNAGVPGPRVPM
                     ACQDVALIDEVCWYFPELKFVTRHGCEPWTDLAVKLLLKWPNLYYSTSAFAPKHYPHD
                     IIQYANTRGADKIIYAGYYPMGLSLERIFKELPDVPFRDHVWPKFLRENAMKLFGIES
                     L"
BASE COUNT          638 a         1278 c         1275 g          666 t
ORIGIN      
        1 ctcgtccggg gcgtaccgct gggccaagac catcttcgaa gccgtcaagg actgacgctt
       61 tcgcccgagc aacgtcttag gcgacggtgc ctgcggtctt gagcttcagg atctcttcgg
      121 tgctgaagcc ccagtcgtgc aacgccgaat cggtcgcgct cccggtcggt gcgggtgcct
      181 tcgggacgtc gggtggcgtg cgggagaagc gcggcgcggg cgccggctgc gcgacgccgt
      241 cgacctcgac gaacgtgcgg cgatgcacgt tgtgttggtg ggcgcgggcc tcggccatcg
      301 tcaacacggg cgcaaagcac acctcgtggc cgcgcatgac ctcgacccac tcgtcgcgcg
      361 tcttcgcctt gaacagctgc gcgacgcgct ccttcaacgc cggccactgg tccttgtcca
      421 tctgctgcgc ggggtcgaga tccgagagct ccatgacgcg caggaactcc acgtagaact
      481 gcggctcgaa cgcggcgacg gagacgtagc cgccgtcggc ggtctcgtag acgccgtacc
      541 agtgtgcacc accgtcgagc aggttcgtgc cgcgcgggcc ccaggcgccc atctggtcga
      601 tcgcgaagaa gatcgacatc aacagcgcgg agccgtcgac catcgccgcg tcgacgacct
      661 gccccgcgcc cgaccgcccg ccctcgacca aggcggcgag cacaccgatg acgagcaaca
      721 tcgagccacc accgaagtcg ccgatgaggt tgatcggcgg gatcggcgga ccgcccgccg
      781 caccgatgtg cgacagcacc cccgacagcg agatgtagtt gatgtcgtgg ccgacgtcac
      841 ccgcgagcgg accgtcttgg ccccacccgg tcatgcgccc gtacacgaga cgaggattgc
      901 gagcgaggca ggcgtcgggg ccgatgccga gccgttcggc gacgccgggc cggaagcctt
      961 cgaccatcac gtccgcgtgc tcgcagcagc gcagcacgac ctccgcgccg cccgggtcct
     1021 tgaggtcgac gccggccgag cggcggccgc ggtcgagcac cgagaacggc ccgaccggcc
     1081 cctgtaagcc cgtgcccgcg cgcacgtgcg cggcgcggtc gaggcgtagc acgtcggcgc
     1141 cgaggtcgga caggatcatg cccgcgtgct ggagcggccc gatgttcggg atctcgagga
     1201 cgcggatgcc ggacagcggt ccactcatgt gacagtcctc tcagtcgagg tgcaagacac
     1261 gcgccgcgtt ctcgtacaag aacttcggcc acacgtggtc acggaacggc acgtgcggca
     1321 gctccgcgaa gatgcggtcg atgtcgagcc ccatcgggta gtagcccgcg tacatcacct
     1381 tgtccgcgcc gcgcgtgttc gcgaaccgga tgatgtgctc gtcgtagtgc ttcggcgcga
     1441 acgccgacgt ggagtagtac aggttcggcc acttcaagag cagcttcacc atcaactcgg
     1501 tccagggctg gccgccgtgg cgcgtcacga tcttcagctc ggggaagaac cagcacacct
     1561 cgtcgagcag gccgacgtac tgcggcgcga aacggatgcg cgggcccggc acgccggcgt
     1621 acacgaagat cgggatgtcg agctccacgc acttggcgta gatcggatac atcttcttgt
     1681 cgtcgagcgg cacctgcggg atgaggccgg tgccccagca ctgtgcgcac ttggcgccgt
     1741 actccttgac tgcgagctcg agcgcgcgca ccgcctccat gccctggttc gggtcgacgt
     1801 tgcacgacgg gaagaaccgg tcgggatagc gctcgagggc ttccttcgag ttgccgcctg
     1861 tcggcaggcc ggtgatcgcc ttgcggatcc cgagctcgtc catcttcgcg acgagcatct
     1921 cgaccggatg gtcgacccgg tcgtagtgcg gcacgccctt gaacatgtat tgcgccggga
     1981 actcccactc ctggctgtca ctgtcgtgcg tctgcgcccg gatgaagtcg tagtgcttcg
     2041 cctgctcctc gcgcggtgcg gggaacccga tcatcgtgtc gatcacaccg accgacgcgc
     2101 gcgccgcctc gaacgcttcg gcactcatga cgtcgtcggc agcttgaaga ccttctgcgc
     2161 gttctcgcgc aggaacttcg gccagacgtg gtcgcggaac ggcacgttcg ggagctcgcc
     2221 gaagatgcgg tcgtaggtga gaccggcggg gaagtagccc gaccagatga tcttgtcggc
     2281 gccgcgggtg ttcgcgtagt cgacgatgtc cttcgggtag tacttcggcg cgaacgcagt
     2341 cgtcgagtag tagaggttcg gccacttgag catcagcttg accgcgagcg cggtccacgg
     2401 ctcacagccg tggcgcgtca cgaacttcaa ctcggggaag aaccagcaga tctcgtcgat
     2461 gtgcgcgacg tcttgcggtt cgaagcgaag gcgcggcccg ggcacgccgg ccgtcgagca
     2521 gaacgcgatg tcgcgctcca cgcacttcgc gtagatcgga tagaacatct tgtcgttcag
     2581 cgcgaccggc ggatacagcc ccgcggggaa cgcgtgcacc gcgcgcagtt cgaactcgtc
     2641 cgcgtaacga tcgatcttgc ggaccgcctc catgccctcg ttcgggtcga tcgagatggc
     2701 gggaaagaag cggtcgggca tgtcgcgcac cgcccggcgg ccgacttcgt cctcgacgcc
     2761 gacgacgacc tgcgacaggc catgctgttc catctcggcg aggacgatca tcaacgggtc
     2821 gtcgatctcg tcctgctccc acttcggcgg gttgtggaac atgtagcccg cgggaaactc
     2881 gaggtcgccc tccttggtcg ccttgtcgag gctggccttg cggatgaagc cgtagaactc
     2941 gtacatgtcc tcgcgcgact tcgggaagcc gagcgcggtg tcgaccgcgc caccgctcgc
     3001 catggccgcg tcgaacgcgg cgttcacaga gactcgatcc cgaagagctt catcgcgttc
     3061 tcgcgcagga acttcggcca cacgtgatcg cggaagggga cgtcgggtag ttccttgaag
     3121 atgcgctcga gtgacaagcc catggggtag tagcccgcgt agatgatctt gtccgcaccg
     3181 cgcgtgttcg cgtactggat gatgtcgtgg ggatagtgct tcggcgcgaa tgccgacgtc
     3241 gagtagtaga ggttcggcca cttcaacagc agcttcaccg cgaggtcggt ccacggctca
     3301 cacccgtgcc tcgtcacgaa cttcagctcg gggaagtacc agcacacctc gtcgatcagc
     3361 gcgacgtcct ggcaggccat cggcacccgc ggacccggca cgcccgcgtt cacgaagatc
     3421 gggatgtcga gctccacgca cttgctgtag agcggataca tcttcttgtc gttgatcggt
     3481 acctgcggcg tcaacccggc cgggaacacc tgtaccgact tcacaccgag ttgctcgacc
     3541 gcctgctcga cctcgcgtac cgcatccatg ccacggttcg ggtcgacgtc ccagcttgcg
     3601 atgaaccgat cggggtgctc ggcgaccgca cggcgcgctt cgaggttcgt cgcgacattc
     3661 agcatgccga cgcgaatgcc gtatgtgtcc atctccgcga ggagtacggc gaccgggtcg
     3721 tcgaggtccg cgaactcggg cacgttcttg aacatgtact gcgcggggaa cgtgaagtcc
     3781 tctttgctct cgcggtcacg cagttgtgcg aggtacttct ggtacttctc cgcctgctcg
     3841 ccggtgggtg cagggtt
//
LOCUS       DASUGA010000736         2167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_218535, whole genome shotgun sequence.
ACCESSION   DASUGA010000736 DASUGA010000000
VERSION     DASUGA010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218535"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VFX21_14540"
     CDS             <1..614
                     /locus_tag="VFX21_14540"
                     /inference="COORDINATES: protein motif:HMM:NF025100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HEX5097241.1"
                     /translation="DVVAEAIAAAGADMALVPTVIGSSIGEAATMIGLLDELWRHHNP
                     MSPADFTMSVHNAASGLISISNKNRGMTTSLAADENTPAAALLEGIGLVSTRNTPVVV
                     ACGDEPAPPTLVQHAPPWSMLAAAVVLAPLSSTATPLATLRIVAGEPPVARPRFDQLL
                     VDNPQFGMLALVDAVLRHTSGVVALDRGTGRGYGALIAPGARA"
     gene            611..1045
                     /locus_tag="VFX21_14545"
     CDS             611..1045
                     /locus_tag="VFX21_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097242.1"
                     /translation="MSRFPPVEDLVPHGPPTLALDEVIDWSDGRTHARLVVRDGGLLV
                     RNGGVDTVVTLELMAQTVAACLGYEAFLHGGGVRVGMVVACRKFAIARTRLDVGERLD
                     IHVVRLRGTDDASTFEAEVHDGRNELVSSAVMTLVHGDRPPD"
     gene            complement(1080..>2167)
                     /locus_tag="VFX21_14550"
     CDS             complement(1080..>2167)
                     /locus_tag="VFX21_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX5097243.1"
                     /translation="FTSAVLVSTLVSFTDTSTGNPTSWQWDFQNDGIVDDTTQNPAFF
                     YPAAGQYTCKLTVADAFSNDTTTQSIEVGIIPVPGFGSTYSNASQTRGFWFQSPRRFS
                     IVSASVPDETSAGIQNVAIYRLAAAPPVYTATASGGLEFVALNQPTTSPIPCVVSFDT
                     GEYVGVLGGCGTTTMHSSYANTSVPFASSVLGQACTLSRFISQFNINTSGANQPYSQA
                     SGSLSRVFLGVTACAAIPYGTGSPSGLGPAAPSMRASALPFVGQAAVHQVTQNDALVL
                     QLMVGGFGRLSIPVPPFGTILIGSIDLIDVMNGGAPVGPGTYPWTFAVPPNPALVGMS
                     INWQNANLVIPTGQWSMSNGIEWWIDV"
BASE COUNT          331 a          746 c          775 g          315 t
ORIGIN      
        1 ccgacgtcgt cgccgaagcg atcgccgcgg ccggcgccga catggcgctc gtgccgaccg
       61 tgatcggctc gtcgatcggc gaagccgcga ccatgatcgg cctgctcgac gagctctggc
      121 gacaccacaa tccgatgtcg cccgccgact tcacgatgag cgtgcacaac gcggcgtcgg
      181 gcctgatctc gatctcgaac aagaaccgcg gcatgacgac ctcgctcgcg gccgacgaga
      241 acacgccggc cgcggcgctg ctcgaaggca tcggcctcgt gtcgacgcgc aacacgccgg
      301 tcgtcgtcgc ttgcggcgac gagcccgcgc cgccgacgct cgtgcaacac gcgccgccgt
      361 ggtcgatgct cgcggcggcc gtcgtgctgg cgccgctgtc gtccactgcg acgccgctcg
      421 cgacgctgcg catcgtcgcg ggtgagccac cggtcgcgcg cccgcgcttc gaccagctgc
      481 tcgtcgacaa tccgcagttc gggatgctcg cgctcgtcga cgccgtgttg cgccacacga
      541 gcggcgtggt cgcgctcgac cgcggcacgg gtcgcggcta cggcgcgctg atcgcgccgg
      601 gagcccgcgc atgagccggt tcccgcccgt cgaggatctc gtgccgcacg gcccgccgac
      661 gctcgcgctc gacgaagtga tcgactggag cgacggcagg acgcacgcgc gactcgtcgt
      721 gcgcgacggc ggcctgctcg tgcgcaacgg cggcgtcgac acggtcgtca cgctcgagct
      781 catggcgcag accgtggccg cgtgtctcgg ctacgaggcg ttcctgcacg gcggcggcgt
      841 gcgcgtcggc atggtcgtgg cctgcaggaa gttcgcgatc gcgcgaacgc ggctcgacgt
      901 cggcgaacga ctcgacatcc acgtcgtgcg cctgcgcggc accgacgacg cgagcacctt
      961 cgaagccgaa gtgcacgacg gccgcaacga gctcgtcagc agcgcggtga tgacgctcgt
     1021 gcacggcgac cgtcccccgg actgaggccg ggggacggag ccgcgccgtc agcgcgcggc
     1081 tacacgtcga tccaccactc gatgccgttc gacatcgacc actggccggt cgggatcacg
     1141 aggttggcgt tctgccagtt gatgctcatg ccgacgagcg ccgggttcgg cggcaccgcg
     1201 aaggtccacg ggtaggtgcc cgggccgacc ggcgcgccgc cgttcatgac gtcgatcagg
     1261 tcgatgctgc cgatcaggat cgtgccgaac ggcgggaccg ggatcgacag acgcccgaag
     1321 ccgccgacca tcagctgcag cacgagcgcg tcgttctgcg tgacctggtg caccgcggcc
     1381 tgaccgacga acggcaaggc actcgcgcgc atcgacggcg ctgccgggcc gaggcccgac
     1441 ggcgagccgg ttccatacgg gatcgccgcg cacgcggtga cgccgaggaa cacgcggctg
     1501 aggctgccgg aggcctgcga gtagggctgg ttggcgccgc tcgtgttgat gttgaactgc
     1561 gagatgaagc gcgagagggt gcaggcctgg ccgagcaccg acgacgcgaa cgggaccgac
     1621 gtgttggcgt aggagctgtg catcgtcgtc gtaccgcagc cgccgagcac accgacgtac
     1681 tcaccggtgt cgaagctcac gacgcacggg atgggactcg tggtcggctg gttcagcgcc
     1741 acgaactcca gaccaccgct cgcggtggcc gtgtagaccg gcggcgccgc agccagcctg
     1801 tagatggcga cgttctggat gccggcgctg gtctcgtcgg gcacgctggc cgacacgatc
     1861 gagaagcgcc tcggcgactg gaaccagaag ccgcgcgtct gcgaggcgtt ggagtacgtc
     1921 gagccgaagc ccggcaccgg gatgatgccg acttcgatcg actgcgtcgt cgtgtcgttg
     1981 ctgaacgcgt ccgcgaccgt cagcttgcac gtgtattgcc cggctgccgg atagaagaag
     2041 gccgggttct gcgtcgtgtc gtcgacgatg ccgtcgttct ggaagtccca ctgccagctg
     2101 gtcggattgc cggtcgacgt gtcggtgaag gacacgagcg tgctcaccag gaccgcggac
     2161 gtgaagc
//
LOCUS       DASUGA010000737         2389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1190144, whole genome shotgun sequence.
ACCESSION   DASUGA010000737 DASUGA010000000
VERSION     DASUGA010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190144"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..809
                     /locus_tag="VFX21_14555"
     CDS             249..809
                     /locus_tag="VFX21_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097244.1"
                     /translation="MLYALATLNDGTKIVTEFEKLKGIRQLAADVFSGKKGFPVWRKV
                     SDVHPISGEPGVLTRRREFISGSVITRLEVVKPKPGFESEDLHQMMPEFFEPDAIMSN
                     LDRNGLWTAPTAQEDLVAGATPGAQYPLHRDPDQRRYVILTWNEDPAAQVRHYLDAER
                     VAGATVDLGAGFSDDDDDEAWGDDVD"
     gene            982..1176
                     /locus_tag="VFX21_14560"
     CDS             982..1176
                     /locus_tag="VFX21_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097245.1"
                     /translation="MAEHTAMRLDAENNCVLARCSTDTSHPHNPSVRVDADPDAEPTT
                     CPYCLQKLTAIVTIDVKEYP"
     gene            1355..>2389
                     /locus_tag="VFX21_14565"
     CDS             1355..>2389
                     /locus_tag="VFX21_14565"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEX5097246.1"
                     /translation="MRSLKIDAKHGRIYASGPMASLLVRRMPGAYHDRNAQVRGGGPT
                     VHLSLTLDTLRDMKTALRMTSQELAACCTEPVMRWARAANTLERDVAAVHDRIAARRF
                     VPLPWEDHVHDPPYAPYEHQRVMATVAVDIPGAAFLCEMGTAKTRSAIEAVAEMIRRS
                     KVDVVFVVCPNSVTGTWAKEIPMWTRTLKAVRLPGTVRERAATIHRLTQDNYVRGTTF
                     LLNYEVLDKLKDDILAVAARLRIAVIFDEGHRIRNSTAKVTKAAMKIAAAAPWRLLMT
                     GTPIVNGIENIWSQWYVVDLGIAFGANYVQFRREFFEENAYTFTLDPLENTAAEVNKR
                     IRRRGVRYKKS"
BASE COUNT          428 a          781 c          828 g          352 t
ORIGIN      
        1 aacccaacgc aacggagcac gacgatgacg acggccgcct acctggaggc gctggcctcg
       61 caacacgagt cgatcatggc gatcgaggac ttcgacgtgc gccacgagga actgcactgg
      121 ttcgagatct gcggctgctg aggttgctgg actgtgcggc ccggaccgcg actgcggccg
      181 ggcgggtcga cgcggcgcgt cccgcgcgac gaccagggac cacgggacgc gaactggagg
      241 atgctcagat gctgtacgca ctcgcgacgc tgaacgacgg cacgaagatc gtgacggagt
      301 tcgagaagct gaaggggatt cggcaactgg ccgccgacgt gttctctggg aagaaggggt
      361 tcccggtgtg gcgcaaggtg tccgatgtcc acccgatcag cggcgagccc ggcgtgctga
      421 cccgtcgccg tgagttcatc tccgggtcgg tcatcacgcg gctcgaagtc gtgaagccga
      481 agcccggctt cgagagcgag gacctgcacc agatgatgcc cgagttcttc gagcccgacg
      541 cgatcatgtc gaacctcgac cgcaacgggc tgtggactgc gccgaccgcg caggaagatc
      601 tggtggcggg cgcgacgccc ggcgcgcagt acccgctgca ccgtgacccc gaccagcggc
      661 ggtacgtgat cctcacgtgg aacgaggacc cggccgcgca ggttcgccac tacctcgacg
      721 ccgagcgggt ggccggcgcg acggtggacc tgggcgcggg gttctcggac gacgacgacg
      781 acgaggcgtg gggcgacgac gtcgactagg acgggccggg tgggcccggg gcgacggttg
      841 ccccgggtct gcccgtttct ggccggcagg gccgcgccta cgcggtccga ggcaggttcc
      901 tggggcggca ggggtgccaa ccccgggggc acggcgggtc cgactcccgc gccggccact
      961 cacgactcaa ctggggggaa cgtggctgaa cacaccgcga tgcgcctgga cgccgagaac
     1021 aactgcgtcc tggcgcgctg ctcgaccgac acgtctcacc cgcacaaccc atccgtgcgc
     1081 gtcgacgccg accccgatgc cgagccgacg acgtgcccgt actgcctcca gaagctgacc
     1141 gcgatcgtga ccattgacgt gaaggagtac ccgtgatggc agcttgccaa ggttcgtaca
     1201 cgacggcaat ccgcagcaac ccaacgacgg gctccgctga gtgcgcggtg tgcggacgcc
     1261 gatgggacaa ggccgatctc gctggcgaga cgaagcggaa cgtgccgctg cattccagca
     1321 actacgaatg agtcctgccc caaacgtcca gcacgtgcgg tcgttgaaga tcgacgccaa
     1381 gcacggccgc atctacgcgt cgggcccgat ggcgtcgttg cttgttcgcc ggatgcccgg
     1441 cgcttaccac gaccgcaacg cccaggttcg cggcggtgga ccgaccgttc atctgtcgtt
     1501 gacgctcgac acgctgcgcg acatgaagac cgcgctgcgc atgacgtcgc aggagcttgc
     1561 tgcgtgctgc accgagccgg tcatgcggtg ggcgcgggcg gcgaacacac tcgagcgcga
     1621 cgtggccgcc gtgcacgacc gcatcgctgc ccgtcggttc gtcccgcttc cgtgggagga
     1681 ccacgtccac gacccgccct acgcgcccta cgagcaccag cgcgtcatgg cgaccgtggc
     1741 ggtggacata cccggcgcgg cgttcctgtg cgagatgggg acggccaaga cgcggtcggc
     1801 catcgaggcc gtcgcggaga tgatccgacg gtcgaaggtc gacgtcgtgt tcgtcgtgtg
     1861 ccccaactcg gtgacaggga cctgggcgaa agagatcccg atgtggacac gcacgctcaa
     1921 ggcggtgcgg ctgccgggca ccgtgcgcga gcgcgctgcc acgattcacc ggctgacgca
     1981 agacaactac gtgcggggca cgacgttcct gctcaactac gaagtgctcg acaaactcaa
     2041 ggacgacatc ctcgccgtcg ccgcgcgctt gcgaatcgcg gtcatcttcg acgaggggca
     2101 ccgcattcgt aactcgacgg ccaaggtgac gaaggcggcg atgaagatag cggctgccgc
     2161 gccgtggcgg ttgcttatga cgggcacgcc gatcgtcaac gggatcgaga acatctggtc
     2221 gcagtggtac gtcgtcgact tgggcatcgc gttcggcgcg aactacgtcc agtttcggcg
     2281 cgagttcttc gaggagaacg cgtacacgtt tacgttggac ccgcttgaaa acaccgccgc
     2341 cgaagtcaac aagcgcatac gtcggcgcgg cgtgcggtac aaaaagtcc
//
LOCUS       DASUGA010000738         2058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_425216, whole genome shotgun sequence.
ACCESSION   DASUGA010000738 DASUGA010000000
VERSION     DASUGA010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425216"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VFX21_14570"
     CDS             complement(<1..557)
                     /locus_tag="VFX21_14570"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX5097247.1"
                     /translation="MEIHGDLGAALSAIEDAIGTDLVLTIGQPPLMRLDGKIAPVPGL
                     KVVDASMMRSYLDDLLEGTYDDLIRDRDVDFAFSHGPHRFRGNVFFQRGLPAVALRLI
                     QQIIPTFDEIGLPHSVRDLISKKQGLILFCGPTGSGKSTSMATMVDAINASRPCHIIT
                     IEDPIEYLHTHRHAVVHQREVGIDA"
     gene            complement(576..1466)
                     /locus_tag="VFX21_14575"
     CDS             complement(576..1466)
                     /locus_tag="VFX21_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097248.1"
                     /translation="MNATRTFNDSGLDVAAGDTVAITAEGNIEFKAGEPSTRVGPEGM
                     PLDECKARLRKDRDLILPAPGQPCWSLIGRVGEKGTPFGIGKSNAVVAKDNGALFLGV
                     NDGQPGDNGGSWKATVTVTPGAAGGADESSSSSPLLFVLIGLGIVALGALLFFALKRR
                     NRAAAEEPAENAAQRQHAAALVGASVGAAAAAPAGAAFDPESTDVNIFKVELSADTLD
                     VGYNFFPDGTRVHWRIMDSGIEVASGEFVAQGGGSEQHFTSLSLDRTLEPSPEAVAVA
                     FNWSINDVPFGYSVRREPRS"
     gene            1720..>2058
                     /locus_tag="VFX21_14580"
     CDS             1720..>2058
                     /locus_tag="VFX21_14580"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX5097249.1"
                     /translation="MADQIAKATVAHADTGNFTVAHNPGFVTGWSPVSAAAVICITLL
                     VLATFLASIGRWAVQIGVPAIIPAVIAGGMAAHLLDRIRYGAVRDFLPTGSLIIDVAD
                     LAVASGIVLLA"
BASE COUNT          316 a          694 c          665 g          383 t
ORIGIN      
        1 ttggcgtcga tgcccacttc ccgttggtgc acgaccgcat gccgatgcgt gtgcaggtac
       61 tcgatcggat cttcgatggt gatgatgtgg cagggccggg acgcgttgat cgcgtcgacc
      121 atggtggcca tgctggtcga ctttccggaa ccggtcggtc cgcagaacag gatcaagccc
      181 tgctttttcg agatcaggtc acgcacggag tgcggcaaac cgatctcgtc gaacgtcgga
      241 atgatctgct ggatgagacg cagggcaacg gccggcaacc cgcgttggaa gaacacgttg
      301 ccgcggaacc ggtgcgggcc gtgcgagaac gcgaagtcga cgtcgcgatc gcggatgagg
      361 tcgtcgtatg tgccttcgag caggtcgtcg aggtagctgc gcatcatgct cgcgtcgacc
      421 accttgagtc cgggcaccgg cgcgatcttg ccgtcgaggc gcatgagcgg cggctgcccg
      481 atcgtcagca cgaggtcggt gccgatcgca tcttcgatcg cgctcagggc cgcgcccagg
      541 tctccgtgga tttccatatc actgttcctt tgttgtcatg accggggctc ccgccgtacg
      601 gagtagccga acggcacgtc gttgatcgac cagttgaagg cgaccgcgac cgcttccggc
      661 gaaggttcga gggtgcgatc gagcgacaag cttgtgaagt gctgttcgct cccgccgcct
      721 tgggcaacga actcaccgga cgcgacctcg atgccggagt ccatgatccg ccagtgcact
      781 cgcgttccgt ccgggaagaa gttgtaaccg acgtcgagcg tgtcggccga gagttccacc
      841 ttgaagatgt tgacgtcggt cgactccgga tcgaaggccg cgcccgccgg cgcggcggct
      901 gcggcgccga cgctcgcgcc gaccagcgcg gccgcgtgct ggcgctgcgc agcgttctcc
      961 gcgggttcct ccgcggcggc gcggttacgt cgcttcagcg cgaagaagag cagcgcgccg
     1021 agcgcgacga tcccgagccc gatgagcacg aacaggagcg gtgaactcga cgacgactcg
     1081 tcggcacctc cggccgcgcc tggtgtcacc gttaccgttg ctttccaact gccgccgtta
     1141 tcaccgggct gcccgtcgtt cacgccgagg aagagcgctc cattgtcctt ggccacgacg
     1201 gcgttgctct tcccgattcc gaagggcgtt cccttctcgc cgaccctgcc gatcagcgac
     1261 cagcacggct gtccgggcgc cgggaggatc agatcccggt ccttgcgcaa gcgagccttg
     1321 cactcgtcga gtggcatgcc ctcgggcccg acgcgtgtcg acggctcgcc cgccttgaac
     1381 tcgatattgc cctcggccgt gatagcgacg gtgtcgcccg ccgcgacgtc gaggcccgag
     1441 tcgttgaacg tcctggtggc attcaccgtg atcgggacag tctctgcgtc tgccctgaag
     1501 cgagccgttt gtgcggcccc agctggggaa acactcgcga gaccccccat cacgcccagc
     1561 gcaaccaccg tctgcagtac acggtgacgt gcccgtggcc gcactccgtg cctacggctt
     1621 ttctgcggtt cagcccgtgg cgttttcacc cgatgcatca ccatgcgccg atctctcgtc
     1681 cgctccctcg cagccatgtc agccgcaacc accgtgattg tggccgacca aatcgccaag
     1741 gcgacggtcg cacacgcgga cacggggaac ttcacggtcg cacataatcc gggcttcgtc
     1801 acgggctggt cgcccgtttc ggccgcggca gtgatctgca tcacactctt ggtgctggca
     1861 accttcctcg ccagcatcgg ccgctgggcg gtgcagatcg gcgttcccgc gatcatcccg
     1921 gcggtcatcg ccggcggtat ggcggcgcat ctcctcgacc gcattcgcta cggcgccgtg
     1981 cgcgacttcc tgccgaccgg ctcgctgatc atcgacgtcg cggatctggc cgtcgcgagc
     2041 gggatcgtgt tgctggcc
//
LOCUS       DASUGA010000739         2576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_78887, whole genome shotgun sequence.
ACCESSION   DASUGA010000739 DASUGA010000000
VERSION     DASUGA010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78887"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VFX21_14585"
     CDS             complement(<1..352)
                     /locus_tag="VFX21_14585"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5097250.1"
                     /translation="MAEAYKSTRRVGAGAAAASGTKALSRELRAQGRKTMNRLLDAGM
                     RVFADKGFHAARVDDIVRAARASHGTFYLYFANKEDLLRALATECATEMDALADRLGP
                     VGPGPDGYATLREFL"
     gene            351..1814
                     /locus_tag="VFX21_14590"
     CDS             351..1814
                     /locus_tag="VFX21_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013425113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5097251.1"
                     /translation="MSSAASVLGLPATIAEAARRFGDRTAYVAEAGWPITYTDVDRIS
                     DEVAVGFARRGLGAGDVLGLVLPPGPEYLIAYCAAAKVGAITAGVNDRLAQRERDAIL
                     DIAGPKLVIDTPAAASLDGVLSDYRVDGEAPPALPYDEDRPVAIIFTSGTTGLPKGAL
                     YTNRQLAFITQTDVGDTWDGGTRSFTGTSFAHLGFMTKLPGNLRRGGTNFIMTRWRAA
                     DALTLLERERMGSVAGVPSQLALMLRQPNFDDYDLSSIKFIIAGGGPITPGLAEEARR
                     RFDAKLSTRYSCTEAGIGLGTAFDDPDEDAVVSVGRPHASVQLAVLDDGDQPVATGEV
                     GQVCLRSPAVMSGYWHDDVATKAAFTADGFVRTGDLGWVDDRGRLRLVGRSKEMYVRG
                     GYNVYPVEVEAVLSTHPDVAAVAIVPRADDVMGEIGVAIVVPRGGAAVALHDLRDFAG
                     PQLAAYKLPEAIETTSELPLTAGEKVDRRALAERFAK"
     gene            1827..>2576
                     /locus_tag="VFX21_14595"
     CDS             1827..>2576
                     /locus_tag="VFX21_14595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5097252.1"
                     /translation="MELDFEPDQEDLRDAIRSVLTKESPVSLARAVVEDDRAPVELWK
                     TFVDLGWPSLTVPEEYGGVGLGPIEAAILAEELGRVIAPGALFPTVTQFVPVVRECGT
                     PDQQARWLGAVAAGECAGALAIGEATASFDPATVGATYRLDGDELELSGTKHHVVEGD
                     TVDELVVVARGDGGVRAIVVPVADVETTREHTLDDSRHLVTVALDGVRVSTDRLLGKG
                     DATDALRRAVEEATLGLALEMVGTAQTILDVT"
BASE COUNT          369 a          920 c          904 g          383 t
ORIGIN      
        1 cgaggaactc gcgcagtgtt gcgtaaccgt cgggcccggg cccgaccggt ccgaggcggt
       61 cggcgagcgc gtccatctcg gtcgcgcact cggtcgccaa cgcacgcagc agatcttcct
      121 tgttcgcgaa gtacagatag aacgtgccgt gcgacgctcg cgcggcgcgc acgatgtcgt
      181 cgacgcgagc ggcgtggaag cccttgtccg cgaacacgcg catgcccgcg tcgagcaggc
      241 ggttcatcgt cttgcggccc tgcgcgcgca gctcccggga caacgccttg gtcccggacg
      301 cggcggctgc ccctgcgccc acgcgtcgtg tcgacttgta cgcttcggcc atgtcctccg
      361 ccgcgagtgt gctcgggctg ccggccacca ttgccgaggc ggctcgccgg ttcggcgatc
      421 gtaccgccta tgtcgccgag gccggctggc cgatcaccta caccgacgtc gaccgcatct
      481 ccgacgaggt cgcggtcggc ttcgcccgcc gcggtctcgg cgcgggcgac gtgctcggcc
      541 tggtgctgcc gcccggcccc gaatacctca ttgcgtactg cgcggccgcc aaggtcggtg
      601 cgatcaccgc gggcgtcaac gaccggctgg cgcaacgcga gcgcgacgcg atcctcgaca
      661 tcgcgggacc gaagctcgtc atcgacacgc cggcggctgc gtcactcgac ggcgtgctct
      721 cggattaccg cgtcgacggt gaagcaccgc ccgcgctccc ctacgacgaa gaccggccgg
      781 tcgcgatcat cttcacgtcg ggaacgaccg gtctgccgaa gggcgcgctg tacaccaacc
      841 gccagctcgc gttcatcacg caaactgatg tcggtgatac gtgggacggc gggacccgtt
      901 cgttcaccgg cacgtcgttc gcgcacctcg gcttcatgac caagctgccc ggcaacctgc
      961 gccgcggcgg caccaacttc atcatgacgc gctggcgcgc ggccgacgcg ctcacgctcc
     1021 tcgaacgcga gcgcatgggc tcggtcgcgg gcgtgccgtc gcagctcgcg ttgatgctgc
     1081 ggcaaccgaa cttcgacgac tacgacctgt cgagcatcaa gttcatcatc gccggcggcg
     1141 gcccgatcac acccgggctc gcggaagaag cgcgccgtcg cttcgacgcg aagctgtcga
     1201 cgcggtattc gtgtaccgaa gcgggcatcg gcctcggcac cgcgttcgac gaccccgacg
     1261 aggatgccgt cgtcagcgtc ggccggccgc atgccagcgt gcagctcgcg gtgctcgacg
     1321 acggcgacca accggtcgcg acgggcgagg tcggccaggt gtgcttgcgc tctcccgcgg
     1381 tcatgtcggg ctactggcac gacgacgtcg cgacgaaggc cgcgttcacc gccgacggat
     1441 tcgtccgcac cggcgacctc ggatgggtcg acgaccgcgg gcgcttgcgg ctcgtgggcc
     1501 gcagcaagga gatgtacgtg cgcggcggct acaacgtgta cccggtcgag gtcgaagcgg
     1561 tgctctcgac gcatcccgac gttgccgccg tcgcgatcgt gccgcgcgcc gacgacgtca
     1621 tgggtgagat cggcgtcgcg atcgtcgtgc cccggggcgg cgcggcggtg gcactgcatg
     1681 acctccgcga cttcgcgggc ccgcagctcg cggcgtacaa gctcccggag gcgatcgaga
     1741 ccacctccga gctaccgctc accgcgggtg agaaggtcga ccgccgtgcg ctcgccgaac
     1801 ggttcgcgaa gtagcgtccc gggctcatgg aactcgactt cgagcccgac caagaagacc
     1861 tgcgcgacgc gatccgcagc gtgttgacca aggagtcgcc ggtctcgctc gcgcgtgccg
     1921 tcgtcgagga cgaccgggcg ccggtcgagc tgtggaagac gttcgtcgac ctcggctggc
     1981 cgtcgctgac cgtgcccgag gagtacggcg gcgtcggtct cggcccgatc gaggccgcga
     2041 tcctcgccga ggagctgggc cgggtcatcg caccgggcgc gctgttcccg accgtgacgc
     2101 agttcgtgcc cgtcgtgcgc gagtgcggca cgcccgacca gcaggcgcgc tggctcggcg
     2161 cggtggcggc cggcgagtgc gcgggtgcgc tcgcgatcgg cgaagcgact gcgagcttcg
     2221 atcccgcgac ggtcggcgcg acgtatcgcc tcgacggtga cgagctcgag ctgtccggca
     2281 cgaaacacca cgtcgtcgaa ggcgacacgg tcgacgagct cgtcgtggtc gcccgcggcg
     2341 acggcggcgt gcgcgcgatc gtcgtgccgg tcgccgacgt cgagacgacg cgcgagcaca
     2401 cgctcgacga cagtcgccac ctcgtcaccg tcgcactcga cggtgtccgc gtgtccaccg
     2461 accggctgct cggcaaaggt gacgcgacgg acgcgctgcg gcgcgcggtc gaggaggcga
     2521 cgctcggtct cgcgctcgag atggtaggta ctgcgcagac gatcctcgac gtcacg
//
LOCUS       DASUGA010000740         3659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1504192, whole genome shotgun sequence.
ACCESSION   DASUGA010000740 DASUGA010000000
VERSION     DASUGA010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504192"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VFX21_14600"
     CDS             <1..585
                     /locus_tag="VFX21_14600"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEX5097253.1"
                     /translation="ELIARSYLEGVDADGPHAEVVVHADLGDLCDENKGEVLRSMACD
                     ARITLSVERDGQPVAVGPPQESIPAKTRRLVGQRDKRRCRFPGCANRRFLHVHHIVHR
                     VFHGTHRSDNLITLCTFHHRLLHVGGWRVRGDADGSVAFIRPDGRRLHERPPDLSGGE
                     TALPYVDARPITMAEGVPLDLVLAVDAILAAMPE"
     gene            658..1377
                     /locus_tag="VFX21_14605"
     CDS             658..1377
                     /locus_tag="VFX21_14605"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5097254.1"
                     /translation="MAWFHAVAEHNHEFQNPTSAAKVRRLGEDLGLGGDSHVLDIASG
                     QGGPATLLAREFGCRLTCVEAYDGFVAVARDRARAAGVTDRIDVVHAKAADFAIEAGV
                     YDAALCLGATFAYGGLDGTLDALVPAVRVGGHVAIGEVFWHAPPPDDIDTHGARSFAE
                     TFAAVDAVAPVVSVIASSADDWDRYESLHYASIEDWLTEHDGEPDAEAIRTQHVYWKN
                     HYARTRGLIGWAVFTGRRTRG"
     gene            complement(1593..>3659)
                     /locus_tag="VFX21_14610"
     CDS             complement(1593..>3659)
                     /locus_tag="VFX21_14610"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5097255.1"
                     /translation="AVEDAHHTPIVIDWRAPVAEPVYRATGVAPMNVVRRRHLVTKKG
                     REVTALDDEVFDQQAIAEAGLEVTGEGALLAALERNRTGRMGDIVATIQSQQDQAIRA
                     DLPGVLVVGGGPGTGKTAVALHRAAYLLYTHRRRLASTGVLLIGPSAVFLRYIEQVLP
                     SLGEQDVQLSTITGLKPRLRIVSDGPEAVTALKGDARMATVIERAVADRERPLPRELV
                     LVIDGLRIRISRHDTARIVDATRRRRGTHNERRPYIARRFHELLVSRYKSALVRNYRE
                     RKIDRPPANVTSLFDRDSSPDPTVAGALSRGEPAPEGWEPELRARLRARPEVKEAIER
                     MWPVLSGPELVNDLLGFSALVRSAADGVLSPDEQLLLHRRHDRDVTNAVWTEADVALV
                     DEADALLGPPEAARPRRKRRRVTDDSIETATRVIEDLGLQGYADAATLVGRYGDNSDH
                     GESLGEPRTYGHVLVDEAQDLTAMQWRMLARRCPSGSATLVGDPGQASRPGAVASWDG
                     VLAHMPQHYPPRFVTLTVNYRTPSEVMDVAARLLAVAAPTVEPSDSVRSTGEEPRFIA
                     ANTDSLIGETVALVREAYERTGTIAVIAPDALHEPLVRALDAYGAVADVADALDAPVA
                     VLDPTSAKGLEFDHVIVVEPSRLVTADRAGLRLLYVTITRTTRSLAIVYTQSLPEGLT
                     PEPVAR"
BASE COUNT          529 a         1233 c         1332 g          565 t
ORIGIN      
        1 gaactgatcg cgcggtcata cctcgaaggt gttgatgccg acgggccaca cgcggaagtg
       61 gtcgtgcacg ccgacctcgg cgacctctgc gacgagaaca agggcgaggt gttgcgatcg
      121 atggcctgcg acgctcggat cacgctgtcc gtcgagcgcg acggccaacc tgttgctgtc
      181 ggaccaccgc aggagtcgat ccccgcgaag acgcgccgct tggtcgggca acgcgacaaa
      241 cgacgatgtc gcttccccgg ctgcgccaat cggcgcttcc tgcacgtgca tcacatcgtc
      301 caccgggtgt tccacggcac gcaccgcagc gacaacctca tcacattgtg cacgttccat
      361 catcgcctcc tgcacgtcgg aggctggcgc gtgcgcggcg acgccgacgg cagcgtcgcg
      421 ttcatccggc ccgacggtcg ccgactgcac gaacggccac ccgacctctc cggcggcgag
      481 accgcgcttc cctacgtcga cgcgcggccc atcacgatgg ccgaaggcgt cccactggat
      541 ctcgtgctgg cagtcgacgc gatcctcgcc gccatgcccg agtgaggccg gggttaccgc
      601 gcggcgggcg ccgcgatatt cgctgcacgc cgcgattggc actcgtacgg tgcgccgatg
      661 gcctggttcc acgcagtcgc cgaacacaat cacgagttcc agaacccgac gagcgcggcc
      721 aaggtgcgcc gactcggcga ggatctcggt ctcggcggcg acagccacgt gctcgacata
      781 gcgtcgggcc agggcgggcc ggcgacgctg ctggcgcggg agttcggatg ccgcctcacg
      841 tgcgtcgagg cctacgacgg attcgtcgca gtcgcgcgag accgtgcacg tgccgcgggt
      901 gtgaccgatc gcatcgacgt cgtccacgcg aaggccgcgg actttgcgat cgaggcgggc
      961 gtgtacgacg cagcgctgtg cctcggcgcg acgttcgcct acggcgggct cgacgggacg
     1021 ttggacgcgc tggttccggc cgtgcgcgtc ggcggccatg tcgcgatcgg cgaggtgttc
     1081 tggcacgcgc cgccgcccga cgacatcgac acgcacggcg cacgctcctt cgccgagacg
     1141 tttgcagccg tcgacgcggt cgcgccggtt gtctccgtga tcgcgtcgtc ggccgacgac
     1201 tgggatcgct acgaaagcct gcactacgcg tcaatcgagg actggctgac cgagcatgac
     1261 ggcgagcccg acgccgaagc cattcgcacg cagcacgtgt actggaagaa ccactacgcg
     1321 agaacacgcg gcctcatcgg ctgggcggtc ttcacgggtc gacgaactcg gggctgagcc
     1381 tcgggcgaat cgactttcgc tcgcgcgcct tcacgcggct cttgccatca aatgtgaaaa
     1441 ggcgcgggtt cgtgcaagga attcgattct gatgacagaa agcgcggtcg gccgcggcac
     1501 ggcgcgatcg cggcggatcg catttgtttc ccgaaggcgc gggattctgc caggaaatcg
     1561 aatttgatga cgaaaagcgc ggagcgcccg tgtcaacggg cgacgggttc gggggtgagg
     1621 ccctcgggga gcgactgcgt gtagacgatc gcgaggctgc gcgtcgtgcg ggtgatcgtg
     1681 acgtacagca agcgcaggcc ggcccggtcg gcggtgacga ggcggctcgg ctccaccacg
     1741 atgacgtggt cgaactccaa gcccttcgcg ctcgtcgggt cgagcacggc gacgggtgca
     1801 tcgagcgcgt cggcgacgtc ggccacggcg ccgtacgcgt cgagcgcgcg cacgaggggc
     1861 tcgtgcagtg cgtcgggggc gatcaccgcg atcgtgccgg tacgttcgta cgcctcgcgc
     1921 acgagcgcga cggtctcgcc gatgagcgaa tcggtgttgg cggcgatgaa gcgcggctcc
     1981 tcgccggtgc tccgtacgga gtccgacggc tccaccgtcg gcgccgcaac tgcgagcagt
     2041 cgcgccgcga cgtccatcac ctcggacggt gtgcggtagt tgacagtgag cgtgacgaag
     2101 cgaggcgggt agtgctgcgg catgtgcgcg agcactccgt cccacgacgc gaccgcgccg
     2161 gggcggctcg cctgtccggg gtcgccgacg agcgtcgccg aacccgacgg gcaccgccgg
     2221 gcgagcatgc gccactgcat cgcggtgagg tcttgggcct cgtcgacgag cacgtgaccg
     2281 tacgtgcgcg gttcgccgag cgactcgccg tggtcggagt tgtcgccgta gcgaccgacc
     2341 agggttgcgg cgtcggcgta gccctggagc ccgaggtctt cgatgacgcg cgtcgcggtc
     2401 tcgatcgaat cgtcggtcac gcggcggcgc ttgcggcggg gccgggctgc ttcgggcggg
     2461 ccgagcagcg cgtcggcctc gtcgacgagt gcgacgtcgg cttcggtcca gactgcgttg
     2521 gtcacgtcgc ggtcgtgacg gcggtgcaac agcagctgct catcgggtga gagcacgccg
     2581 tcggccgcgg agcgcacgag cgcgctgaag ccgagcaagt cgttcaccag ctccgggccc
     2641 gacagcaccg gccacatgcg ctcgatcgct tccttcacct cgggccgtgc tcgcaagcgc
     2701 gcccgcagct cgggctccca gccttcgggc gccggttcgc cgcgtgacag cgcaccggca
     2761 acggtcggat cgggtgacga gtcgcggtcg aacagactcg tgacgttcgc cggcggccgg
     2821 tcgatcttgc gctcgcggta gttgcggacg agcgcggact tgtagcgcga caccaacagc
     2881 tcgtggaacc gccgcgcgat gtacgggcgc cgttcgttgt gcgtgccgcg ccggcgccgg
     2941 gtcgcgtcga cgatgcgggc ggtgtcgtgg cggctgatgc ggatgcgaag accgtcgatc
     3001 acgaggacca gctcgcgtgg cagcgggcgt tcgcggtcgg cgaccgcgcg ctcgatgacc
     3061 gtcgccatgc gcgcgtcacc cttcagtgcg gtgaccgctt ccgggccgtc gctcacgatc
     3121 cgcaggcgcg gcttgagccc cgtgatcgtg ctgagctgca cgtcttgctc gccgagactc
     3181 ggcagcacct gctcgatgta gcggaggaac accgcgctcg ggccgatgag cagcacgccg
     3241 gtcgacgcca gccggcggcg gtgcgtgtag aggaggtacg cggcgcggtg cagcgcgacc
     3301 gcggtcttgc cggtcccggg tccgccgccg acgacgagca caccgggcag gtcggcgcgg
     3361 atcgcctggt cttgttgcga ctggatcgtg gcgacgatgt cgcccatgcg gccggtgcgg
     3421 ttgcgttcga gcgcggcgag cagcgcgcct tcgccggtga cctcgagccc ggcctccgcg
     3481 atcgcctgct ggtcgaacac ctcgtcgtcg agcgcggtga cctcgcggcc cttcttggtg
     3541 accaggtggc ggcggcgcac gacgttcatc ggcgccaccc cggtcgcccg gtagaccggc
     3601 tcggcgacgg gcgcgcgcca gtcgatgacg atcggggtgt gatgggcgtc ctcgacggc
//
LOCUS       DASUGA010000741         2822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1989738, whole genome shotgun sequence.
ACCESSION   DASUGA010000741 DASUGA010000000
VERSION     DASUGA010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1989738"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VFX21_14615"
     CDS             complement(<1..366)
                     /locus_tag="VFX21_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097256.1"
                     /translation="MTKAIPAAIPPRLSAPVRGSSVVEAVVGVVVPSVVTVASVLPVF
                     LTVVDVVPSVPTVELELPSVVDVVVAGCVVVVVANAVVVVVVGSVEVVDVLVLVEVEV
                     LVEVDGLVLDDDVLDDDVLD"
     gene            548..1855
                     /locus_tag="VFX21_14620"
     CDS             548..1855
                     /locus_tag="VFX21_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097257.1"
                     /translation="MAVAVVAGVSACKPPSGPNDIDRNREHYVLFAYDHLQFKGSEKT
                     EVHGGHIGVNNKFSGKNGEVSLSMCSSSNPTDLDGSKLKKIGADLVGGQQIVADTARI
                     GANCKNDKTPPDAGYFFGDPTNVTAPFLGKFALIPQDPAQPNGLIVSDPLHGWNEATW
                     DANQKPFEATDCRRFAKAGGLGDPVTYTYKNQSTFVLAPDTGVNAPVPGGTKAVQLGS
                     AGAKSGPFGDVNVPSGDVLDLRVPAGQTGIFTFCDLSVNANSVILTSEGTIIRVVEKF
                     SIDGGDFGFASPADAQSPDAAACTVQSEAKVYVLGANVKSSVSTVSLGKQSVMKAQIW
                     SKSGELDLGHGTKFQGHIWAPSMHSDWGISINECGSGPEPTTTTTSTTTTTTTTVGPT
                     SSSTSTTTVAETSTSTSTSTSTSTSTTTTPEGPTTTEETLPDP"
     gene            2071..2289
                     /locus_tag="VFX21_14625"
     CDS             2071..2289
                     /locus_tag="VFX21_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097258.1"
                     /translation="MALLAALLALALVVGAAAGDLLSPSDPRSESKFSTAVSYSTDIN
                     SYGSRSTSWPSRVATRRTRRRSAAATSV"
     gene            2622..>2822
                     /locus_tag="VFX21_14630"
     CDS             2622..>2822
                     /locus_tag="VFX21_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097259.1"
                     /translation="MGFGKRSTVYAQMWAWNAEVSLGNNTNLFGKFWFRSGHSDFGME
                     VNDCPQRTCSPGGSTTTTTTRPA"
BASE COUNT          560 a          943 c          942 g          377 t
ORIGIN      
        1 gtcgagcacg tcgtcgtcga gcacgtcgtc gtcgagcacg agcccgtcca cctcgaccag
       61 cacctcgacc tcgacgagca cgagcacgtc gaccacctcg acgctaccga cgaccacgac
      121 gacaacggca ttggcgacga cgaccacgac gcagccggcg acgacgacgt cgacgacgct
      181 cggcaactcg agctcgacgg tcggcacgct cggcacgaca tccacgacgg tcaagaacac
      241 gggaagcacc gaagcgacag tcacgacact cggaacgacg acaccgacga ccgcctccac
      301 gactgagctg ccccgcacgg gtgccgacag tcgcggcggg atggccgccg gtatcgcctt
      361 cgtcatcgcc ggggccgccc tcctcggctt gggtcgccgc aagacagcga cctcgtaagt
      421 catcgcggcg cggcccacca cgccgaggcg acttcgcaag cccacccgca ggatgcaaca
      481 aggggaacgg atgacaacag ggggcctggg taggggagcg cgtcggcgcg cgctcatatt
      541 cacgggattg gccgttgcag tcgtcgcggg cgtgagcgcg tgcaaaccgc cgagcggccc
      601 gaacgacatc gaccgcaacc gcgagcacta cgtgctgttc gcgtacgacc acttgcagtt
      661 caagggcagc gagaagaccg aggtccacgg cggacacatc ggcgtcaaca acaagttcag
      721 cggcaagaac ggcgaggtct cgttgagcat gtgctcgtcg agcaacccga ccgacctcga
      781 cggctccaag ctcaagaaga tcggggccga cctcgtgggc ggtcagcaga tcgtcgccga
      841 caccgcgcgc atcggtgcga actgcaagaa cgacaagact ccgcccgatg cggggtactt
      901 cttcggtgac ccgaccaatg tcaccgcacc cttcctcggg aagttcgcgc tcatcccgca
      961 ggacccggcg cagcccaacg gcctgatcgt gagtgacccg ctccacggat ggaacgaagc
     1021 gacgtgggac gcgaaccaga agccgttcga agcaaccgac tgccgccgct tcgcgaaggc
     1081 gggtgggctc ggcgacccgg tcacgtacac gtacaagaac cagtcgacgt tcgtcctcgc
     1141 ccccgacacg ggtgtgaacg cgccggtgcc gggcggcacg aaggccgtgc agctcgggtc
     1201 ggcgggcgcg aagtcgggtc cgttcggtga cgtcaacgtg cccagcggag acgtgctcga
     1261 cctgcgcgtc ccggccggcc agaccgggat cttcacgttc tgcgacctgt cggtcaacgc
     1321 caactcggtc atcctgacga gcgagggaac gatcatccgg gtggtcgaga agttctcgat
     1381 cgacggcggc gacttcggct tcgcgtcgcc ggccgacgcg cagagcccgg acgcggcggc
     1441 gtgcacggtc cagtccgagg cgaaggtcta cgtgctcggt gccaacgtga agtcgagcgt
     1501 gtcgacggtc tcgctcggca agcagagcgt gatgaaggcg cagatctggt cgaagtcggg
     1561 tgagctcgac ctcggtcacg ggaccaagtt ccagggccac atctgggcgc cgtcgatgca
     1621 cagcgactgg ggcatcagca tcaatgagtg cggctcggga cccgagccca cgacgaccac
     1681 gacttcgacg acgaccacca ccaccaccac agtggggccg acgtcgtcgt cgacatcgac
     1741 gaccacggtg gcggagacgt ccacgagcac gtcgacgtcg acgagtacga gcacgtcgac
     1801 gaccacgacg ccggagggtc cgaccacgac cgaggaaacg ctgccggatc catagaccga
     1861 catcgagcgg cgtcaggcgg ccggtgcttg cgtgcgtacc ggccaccgat gccgcacgaa
     1921 ggcaactgca agtgcaagta ccggcaaccg aatgatcagg caccgacagg ggggaaccga
     1981 gtcgttcccg ttctctgatc cgtccagtgg acgaagagaa gggaacggaa catgtcagga
     2041 atcatccgtg tccgaggagg ccggcggcgc atggcgctac tcgcggcgct cctcgcgctc
     2101 gcgttggtcg tcggcgcggc cgccggtgat ctgctctcac cgagcgatcc gcggtcggag
     2161 tcgaagttct cgaccgcagt gagctacagc accgacatca acagctacgg ttcgcgctcg
     2221 acgagttggc cgtcaagggt ggcgacacga cgcacccgtc gaagatccgc ggcggcaaca
     2281 tcggtgtgaa cgcggccggc gagatcggcg gcaaggcccg tctgcggatg tgcaacgggt
     2341 ccggcaacgg aacccgatcc aggtcgacga gggcagcctc gtcgccgcgg acagcatcga
     2401 catccagcgc gcgtgcacgc tgtgggacgt ccgttcgggc gtgtacacgc accagagcag
     2461 cccgcgtgcg ttccggatgg ctgtcggtca gccgtggtac ccgatcaaca ccgtgaaccc
     2521 gccgccgatg attacgaacc tgccggccaa gccgtcgatc acttgcgcga cgggcaccaa
     2581 ggcgacgtgg aagaccggta cgcccgcgcc cgggaacgca ggtgggcttc ggcaagcgct
     2641 cgacggtcta cgcgcagatg tgggcgtgga acgcggaggt cagtctcggc aacaacacga
     2701 acctgttcgg gaagttctgg ttccggtcgg ggcacagtga cttcggcatg gaggtgaacg
     2761 actgcccgca acgcacatgc tcgccgggtg gatcgacgac gacgaccacg acgcgtccgg
     2821 ca
//
LOCUS       DASUGA010000742         2340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_44199, whole genome shotgun sequence.
ACCESSION   DASUGA010000742 DASUGA010000000
VERSION     DASUGA010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44199"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="VFX21_14635"
     CDS             complement(<1..154)
                     /locus_tag="VFX21_14635"
                     /inference="COORDINATES: protein motif:HMM:NF014077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX5097260.1"
                     /translation="MRILCTNDDGVESEGLHVLAAALADAGHDVVVVAPSSDRSGASA
                     AIGVLHA"
     gene            187..1257
                     /locus_tag="VFX21_14640"
     CDS             187..1257
                     /locus_tag="VFX21_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006546051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEX5097261.1"
                     /translation="MIKYIGSKRRLVPVLGELCTASGARTALDLFTGTTRVAQEFKQR
                     GAHTTAVDSARYSEMFARCYVSLDAATVDLAAIEGALADLMARPDVDGYVTETFCRAS
                     RFFQEHNGRRIDAIRDAIEHDYRESWLYPVLLTSLIEAADRVDSTTGVQMAFVKQWAP
                     RSFRPLELRVPEMVAGPGRALRADALDAAAELGPFDLAYLDPPYNQHRYFTNYHVWET
                     LVAWDAPVHYGVACKREDARDAATKSVFNDRRRMPDALAAVIAKVDAAVVIVSYNDES
                     WIAIDDLEAMAATRGRVVTLAFPAKRYVGAQIGIHNPQGEKVGSVSHLRNQEYVLIAG
                     DATRVARTVAPLARYARVPGGV"
     gene            1287..>2340
                     /locus_tag="VFX21_14645"
     CDS             1287..>2340
                     /locus_tag="VFX21_14645"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5097262.1"
                     /translation="MDPGVVRQWDAVAGDPGALASLMDALPVDVMLVEVGEDGSLVCR
                     AANRHLAELCGCAPPEMRGQPIEKLMSDGAHEHLLAAIHDAVATGRPARALVINPDVG
                     DGRRVLHANAIPLAHGGMPRFVLAMFDDTEGRAASDLIAADLGSALDAAPDIVLRYDM
                     DLRLLYLNRAAERMLGRPRAELLRRSNLEAGLPPNVAERWDVALRSVAVASEHEDLEF
                     ALETPEGHRWFHARLAPEYDGGTRVSSIIATCRDFTEQRLLADALAHLAMHDSMTQLP
                     NRRMLLERLQPASARARRTGRAVALLFVDLDDFKFVNDTLGHRAGDAVLRETAKRIAG
                     ASRAGDMVVRFGGDEFV"
BASE COUNT          354 a          845 c          779 g          362 t
ORIGIN      
        1 cggcgtgcag cacgccgatc gccgcactcg ccccactgcg atccgagctc ggcgcgacca
       61 cgacgacgtc gtggccggcg tccgccagcg cggccgccaa cacatggagc ccttcggact
      121 cgacgccgtc gtcgttcgtg cagaggatgc gcacggcggc accctagggc acctaccctc
      181 cgtcccgtga tcaagtacat cggatcgaag cggcgtctcg tccccgtact cggcgagctg
      241 tgcacggcgt ccggcgcgcg caccgcactc gatctgttca ccggaacgac acgtgttgcg
      301 caggagttca aacagcgcgg cgcacacacc acggctgtcg acagcgcacg ctattccgag
      361 atgtttgccc gttgttacgt ttccctcgac gcggcgacgg tcgacctcgc cgccatcgaa
      421 ggcgccctcg ccgacctcat ggcccgtccg gacgtcgacg gatacgtgac cgagaccttt
      481 tgtcgcgcct cacgcttctt ccaagagcac aacggccgcc gcatcgacgc catccgcgac
      541 gcgatcgagc acgactaccg cgagtcgtgg ctgtatccgg tgctgctgac gagcctgatc
      601 gaagcggctg accgcgtcga ctccaccacc ggcgtacaga tggccttcgt caagcagtgg
      661 gcgccgcgct cgttccggcc cttggagctg cgggtgccgg agatggtggc cggccccgga
      721 cgggcgctgc gggcggacgc cctcgacgcg gcggcggagc tcgggccctt cgacctcgcg
      781 tatctcgatc cgccctacaa tcagcatcgc tacttcacca actatcacgt ctgggagacg
      841 ctcgtcgcgt gggacgcgcc cgtgcactac ggcgtcgcct gcaagcgcga ggacgcgcgt
      901 gacgccgcga cgaagagcgt cttcaacgac cggcgccgca tgccggatgc actggccgcg
      961 gtgatcgcca aggtcgacgc ggcggtcgtg atcgtctcct ataacgacga gtcgtggatc
     1021 gcgatcgacg acctcgaagc gatggccgcg acccgtggtc gcgtggtgac gctggcgttc
     1081 cccgcgaaac ggtacgtcgg cgcccagatc ggtattcaca acccgcaagg tgagaaggtc
     1141 gggtcggtct cgcacctgcg caaccaggaa tacgttctga tcgcgggcga cgccacgcgc
     1201 gtcgcgcgga cggttgcccc gcttgcccga tacgcgcgtg tacccggagg cgtgtgaatc
     1261 ccgggcaata gggaacaacg ctgtgaatgg acccgggtgt ggtgcggcag tgggacgcgg
     1321 tcgcgggcga tccgggtgct ctcgcgtcct tgatggacgc gcttccggtc gacgtcatgc
     1381 tggtcgaggt cggtgaggac ggttcgctcg tctgccgcgc ggccaatcgc catctcgcgg
     1441 agctctgcgg atgcgcgcca ccggagatgc gcggccaacc catcgagaag ctcatgagcg
     1501 acggcgcgca cgagcatttg ctcgccgcga tccatgacgc ggtcgcgacc ggccgtcccg
     1561 cgcgcgcgtt ggtgatcaac ccggacgtgg gcgacggtcg tcgcgtcctg cacgcgaacg
     1621 ccatcccgct ggctcacggc ggcatgccgc gcttcgtgct ggccatgttc gacgacaccg
     1681 agggccgcgc cgcgtccgac ctcatcgcgg ccgacctcgg cagcgccctc gacgccgcgc
     1741 ccgacatcgt cctgcgctac gacatggacc tgcgcctgct ctatctcaac cgtgccgccg
     1801 aacggatgct cggccggccc cgcgccgaac tgttgcgccg ttccaacctc gaggccgggt
     1861 tgccgcccaa cgtcgccgaa cgatgggacg ttgccctccg gtcggttgcg gtcgccagtg
     1921 agcacgagga cctggagttc gcgctcgaga cgcccgaggg ccaccgctgg ttccacgccc
     1981 gcctcgcgcc cgaatatgac ggcggcaccc gggtcagctc catcatcgcg acgtgccgcg
     2041 acttcaccga gcaacgcctg ctcgcggacg cgctcgcgca cctcgccatg cacgactcga
     2101 tgacgcagct gccgaaccgg cgcatgctgc tcgaacggct gcagcccgcg agcgcgcggg
     2161 cccgccgcac cgggcgcgcg gtggcgctgc tcttcgtcga cctcgacgac ttcaagttcg
     2221 tcaacgacac gctcggtcac cgcgcgggcg atgcggtgct gcgcgagacc gcgaagcgga
     2281 tcgcgggcgc gagccgcgcc ggcgacatgg tcgtcaggtt cggcggtgac gagttcgtcg
//
LOCUS       DASUGA010000743         2305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_564976, whole genome shotgun sequence.
ACCESSION   DASUGA010000743 DASUGA010000000
VERSION     DASUGA010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564976"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="VFX21_14650"
     CDS             <1..728
                     /locus_tag="VFX21_14650"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5097263.1"
                     /translation="SAPTTGASSSATTAAASLIAQEITFTGPNGALTAAYASPPDPKG
                     AVLVCHENRGLTPHFYDVVGRLAQAGYAAMCVDLVSEEGGTASMDEGAVQGALGGAPE
                     DRLVGDLQAGIHELQTRAPGVKVGTVGFCFGGSMVWNLLNAGEDRIVAAVPFYGPAPA
                     DPDFSGADAAVLAIYGELDSRVNASQESVSQALDAAGLTHETKVYPGADHAFFNDTGP
                     RYNEAAAKDAYDRLLAWFDQYLS"
     gene            complement(742..1401)
                     /locus_tag="VFX21_14655"
     CDS             complement(742..1401)
                     /locus_tag="VFX21_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX5097264.1"
                     /translation="MDVYEALYTTRAMRRVRPDPIPMDVQARLLDAAVRAPSGGNMQN
                     WRFMLVDDPAMKAQLGPLYRECLGMLWQTVYKDRADAAAARPDDPESKTFVVMQRSAQ
                     HLADHFEEYPLLLFSFAQFDPSGGSIHPATWSAMLAARAEGIGSTLTSVMIFKLDDML
                     SILGVPKDQGWIFASCVCFGYPTGKWGVAPRRPVHEVASRNGWDAPLGLEIPEPLWPR
                     P"
     gene            1508..>2305
                     /locus_tag="VFX21_14660"
     CDS             1508..>2305
                     /locus_tag="VFX21_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097265.1"
                     /translation="MGTTDIEPAARYTLDFANTEVGAISFTDAFRQPTFAVAPVEVSL
                     VEREPEPRPAPVALASVEVAPPVDFADAVAPAVDVAPVEVAPVEVAPVEVAPVEVARE
                     QEHFSDEAPVSAEPQEAIMASEPEPAVAPWLVLDNPDIATHAHEVIAAVAQAEAELRD
                     AQVELEQAESAALAATEHEVDVRRRVEKTEYDVHQQGTVLDRQTEELLRARALVLDKE
                     LEARRFELSEREQMELAVAAQRRAQAEDDARRIAEEARAAAAAAEARI"
BASE COUNT          386 a          763 c          827 g          329 t
ORIGIN      
        1 cgtcggcccc gaccaccggt gcttcgtcgt cggcgacgac ggctgcggcg tcgctcatcg
       61 cgcaggagat cacgttcacc ggtccgaacg gtgcgctcac cgcggcctac gcatcgccgc
      121 ccgatccgaa gggtgcggtg ctcgtgtgtc acgagaaccg cgggttgacg ccgcacttct
      181 acgacgtcgt cggccggctc gcgcaggccg gctacgcggc gatgtgtgtc gacctcgtct
      241 ccgaagaggg cggcaccgcg tcgatggacg aaggtgccgt gcagggagcg ctcggcggcg
      301 cacccgaaga ccgtctcgtc ggcgacttgc aggcgggcat ccacgagttg cagacgcgtg
      361 cgccgggcgt gaaggtcggc accgtggggt tctgcttcgg tgggtcgatg gtgtggaacc
      421 tgttgaacgc gggcgaggat cgcatcgtcg cggcagtgcc gttctacggt cccgcgcccg
      481 cggatccaga cttcagtggc gcggacgcgg cggtgctcgc gatctacggc gagctcgaca
      541 gccgcgtcaa cgcgtcccag gagtcggtgt cacaggcgct cgacgcggcc gggctcaccc
      601 acgagacgaa ggtgtacccc ggtgccgacc acgcgttctt caacgacacc ggaccgcgct
      661 acaacgaagc cgcggcgaag gacgcgtacg accgcctgct ggcgtggttc gaccaatacc
      721 tcagctgagg taaccactgg ctcacgggcg cggccagagc ggttcgggga tctcgaggcc
      781 gagcggcgcg tcccagccgt tgcgcgacgc gacttcgtgc accggccggc ggggcgcgac
      841 gccccacttg cccgtgggat agccgaagca cacgcacgac gcgaagatcc agccttggtc
      901 cttggggacg ccgaggatcg acaacatgtc gtcgagcttg aagatcatga cggacgtcag
      961 ggtcgagccg atgccctcgg cgcgcgcggc gagcatcgcc gaccaggtcg caggatggat
     1021 cgacccgccc gacggatcga actgggcgaa gctgaacaac aggagcgggt actcctcgaa
     1081 gtggtccgcg agatgctgcg ccgaccgttg catgacgacg aacgtcttgc tctccgggtc
     1141 gtccggtcgc gccgcggcgg cgtcggcgcg gtccttgtag acggtctgcc acagcattcc
     1201 gaggcattcg cggtacagcg gcccgagttg tgccttcatt gccgggtcgt cgaccagcat
     1261 gaaccgccag ttctgcatgt tgccgccgct gggcgcgcgc acggccgcgt cgagcagccg
     1321 cgcctgcacg tccatcggga tgggatccgg tcgaacgcgc cgcatggctc gggtcgtgta
     1381 gagcgcttcg tagacgtcca taccgaggag cctggcacga acgtttcgct ctgaaccaat
     1441 atggacacac caaagcgatt tggcggcgag tgatcgcaaa cagtgatcgc aaacagatag
     1501 gcccaggatg ggaaccaccg acatcgagcc ggccgctcgg tacacgctgg acttcgcgaa
     1561 cacggaagtc ggtgcgatct cgttcaccga cgcgttccgc cagcccacgt ttgcagtcgc
     1621 gccggtagaa gtctcgttgg tcgagcgcga gccggagccg cgacctgcgc ccgtcgcgct
     1681 cgcgtccgtc gaagtcgcac cgccggtcga cttcgcggac gcggtcgcgc cggcagtgga
     1741 tgtcgcgccg gtggaagtcg cgccggtgga agtcgcgccg gtggaagtcg cgccggtgga
     1801 agtcgcacgc gagcaggagc acttctcgga cgaggcaccg gtctcggccg agccgcagga
     1861 ggcgatcatg gcgagcgaac ccgagccggc ggtcgcgccg tggctcgtac tggacaaccc
     1921 cgacattgcg acgcacgcgc acgaagtcat cgccgcggtc gcacaggccg aagctgagct
     1981 gcgcgacgcg caggtggagc tcgagcaggc ggagtccgcg gcgctggcgg cgaccgagca
     2041 cgaggtcgat gtgcgccggc gggtcgagaa gaccgaatac gacgtgcacc agcagggcac
     2101 ggtgctcgac cggcaaaccg aagagctgtt gcgtgcccgt gcgctcgtgc tcgacaaaga
     2161 gctcgaagcg cgccggttcg agctgtcgga acgcgagcag atggagctgg ccgtcgccgc
     2221 ccaacgacgc gcgcaagccg aggacgacgc gcgccgcatc gccgaggagg cacgcgccgc
     2281 ggccgcggcg gccgaggccc ggatc
//
LOCUS       DASUGA010000744         2960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1053233, whole genome shotgun sequence.
ACCESSION   DASUGA010000744 DASUGA010000000
VERSION     DASUGA010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053233"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VFX21_14665"
     CDS             <1..535
                     /locus_tag="VFX21_14665"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX5097266.1"
                     /translation="FWEHSMAVAAGASIAARFAHVSPGDALCAGLLHDLGSALLYRLD
                     RDHYNARLAQGLDPQAYLASEHDAYGGDHAVIGAFALDAWKLPVSICDAIRNHHRDPT
                     SVDDKLTRVVIAGEALASAAYCDPETGVRPHGHEPVVDPTDAFRAMGLTAISVENLVR
                     RTAEEAEALDAVLSSVR"
     gene            624..962
                     /locus_tag="VFX21_14670"
     CDS             624..962
                     /locus_tag="VFX21_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097267.1"
                     /translation="MSMRTDCKHYDSRTYPSGDVVRKCRIDLAPEAPWRCPADCHGYE
                     RRMIDVGWQYGSLGDAQGAPEPAPAAGDDVARLLDEAEEIVNTAGFELQAEQARKQKK
                     RLFRKKGKRR"
     gene            992..1570
                     /locus_tag="VFX21_14675"
     CDS             992..1570
                     /locus_tag="VFX21_14675"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX5097268.1"
                     /translation="MLCPWCYVAAVRIRRVEAEAGDALRVEWRSFMLRPQPEARSLEK
                     FRRYARSWARPAAAEPDAAFNLWSSDAAPPSHSLPPLVATKVAASFGDDAFAKYHHAL
                     FRAYFVDNRTVSDSSVLREVAAGVGLDGAEFATRFERDNGEYAAAVVADHRAALAEGI
                     AAVPSLMVDHEHVLTGALATDAYRRIVARRSG"
     gene            complement(1634..2488)
                     /locus_tag="VFX21_14680"
     CDS             complement(1634..2488)
                     /locus_tag="VFX21_14680"
                     /inference="COORDINATES: protein motif:HMM:NF012948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEX5097269.1"
                     /translation="MSNSQSSRRIRVGKFVIRGGLAALVGAGGLALTAGVAAAHHTTL
                     TATTSCVNAQGQWTVTWKVENSEDNKVMNITEVELWTDNNGTGNPHNDEGTPPTFAPS
                     PVPASGSASGVSTSPFGNSGTIARVSIDVDVSWSGGYTDSEELTINKPGACPATTTTT
                     WATTTTTKPATTTTTWATTTTTYPTTTTTEAPTTTTTEAPTTTTMPEETTTTTTGAQA
                     TTTSTVQAAGGTVTTAPTTTIATTDPAPTSELPRTGSASVLLAILGVLMLAGGSLLVR
                     VSRPNTAN"
     gene            complement(2789..>2960)
                     /locus_tag="VFX21_14685"
     CDS             complement(2789..>2960)
                     /locus_tag="VFX21_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019049435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BldC family transcriptional regulator"
                     /protein_id="HEX5097270.1"
                     /translation="TLLRPAEVAALANVDRKTVARWAESGRLPSFRTLGGHRRFRLHE
                     VLAALENASREQ"
BASE COUNT          438 a         1020 c         1018 g          484 t
ORIGIN      
        1 cttctgggag cactcgatgg cggtggcggc cggcgcgtcg atcgcggcgc gcttcgcaca
       61 cgtctcgccc ggcgatgcgc tgtgtgcggg actgctgcac gacctcggat cggccctgct
      121 gtaccggctc gaccgcgacc actacaacgc gcgcctcgcg cagggtctcg atccgcaggc
      181 atacctcgcg tcggagcacg acgcgtacgg cggggaccac gcggtgatcg gcgcgttcgc
      241 cctcgacgcg tggaagctgc cggtgtcgat ctgcgacgcg atccgcaacc accaccggga
      301 cccgacgagc gtcgacgaca agctcacgcg ggtcgtcatc gccggtgaag cgctcgcgag
      361 cgcggcatac tgcgacccgg agaccggcgt ccgtccccac gggcacgaac ccgtcgtcga
      421 cccgacggat gcgttccggg ccatggggct cacagccatc tcggtggaga atctggtccg
      481 gcgcacagcc gaagaggccg aagccctcga cgccgtgctg tcctccgtgc gctgaagatt
      541 ctccggtcag gccccccggc accgccccgt tgggcctagt ccgcgagacc acatgcgcct
      601 cgcgcattcg cgacaatggt cccatgtcca tgcgcaccga ctgcaagcac tacgacagcc
      661 gcacgtaccc gtcgggggac gtcgttcgga agtgccgcat cgacctcgcg cccgaagcgc
      721 cctggaggtg cccggccgac tgccacggct acgagcggcg catgatcgac gtgggctggc
      781 agtacgggtc gctgggcgat gcgcaggggg cgcccgaacc ggcgcccgcc gccggcgacg
      841 acgtcgcccg tttgctcgac gaagcggaag agatcgtcaa cacggccggc ttcgagctgc
      901 aagccgagca agcacgcaag cagaagaagc gcctcttccg caagaagggc aagcggcgct
      961 gactccggtt gccttcaccg tctggtcgga catcctctgc ccgtggtgtt acgtcgcggc
     1021 ggtgcgtatc cgacgagtgg aggccgaagc cggcgacgcg ctgcgcgtcg agtggcgctc
     1081 gttcatgttg cgaccgcaac ccgaagcgcg ctcgctcgag aagttccgca ggtacgcgcg
     1141 gtcgtgggcc cgtccggccg cggccgagcc cgatgcggca ttcaacctgt ggtcctccga
     1201 tgccgcgcca ccgagtcaca gcctgccgcc gctcgtcgcg accaaggtgg ccgcgtcatt
     1261 cggtgacgac gcattcgcga agtaccacca tgcgttgttc cgtgcctatt tcgtcgacaa
     1321 ccgaacggtc tccgactcga gcgttctgcg cgaggtcgca gcgggcgtcg gcctcgacgg
     1381 cgccgagttc gcaacgcgct tcgaacggga caacggcgaa tacgcggccg ccgtcgttgc
     1441 cgaccaccgc gccgcgctcg ccgagggcat cgcggccgtg ccgtcgctga tggtcgacca
     1501 tgagcacgtg ctgaccggcg cgctcgccac cgacgcctac cgccgcatcg tcgcgcgccg
     1561 ttcggggtga gaacgcgcct cgacgagcgc cggatcggcg ctcgtcgagg ccagttccgc
     1621 aggggagcgg aaatcagttc gcagtgttcg gtcgcgacac ccgcacgagg agcgagccac
     1681 cggcgagcat cagcacaccg aggatcgcca gcaggacgga cgcactaccg gtgcgcggca
     1741 gctcgctcgt cggcgccgga tcggtcgtgg cgatcgtggt cgtcggcgct gtcgtcaccg
     1801 tgccgccggc cgcctgcacc gtgctggtcg tcgtggcctg cgcaccggtc gtcgtggtcg
     1861 tcgtctcctc cggcatcgtg gtcgtcgtcg gtgcctccgt cgtcgttgtc gtcggcgcct
     1921 cggtcgtcgt cgtggtcggg tacgtcgtgg tcgtcgttgc ccacgtcgtc gtcgtagtgg
     1981 cgggcttcgt cgtcgtggtg gtcgcccacg tcgtcgtggt cgtcgccggg cacgcaccgg
     2041 gcttgttgat ggtcagctct tccgagtcgg tgtaaccgcc cgaccagctg acgtcgacat
     2101 cgatggagac tcgggcgatc gtgcccgagt tgccgaacgg cgatgtcgac acgccggatg
     2161 cgctgccgct ggcgggtacg ggtgacggcg cgaacgtcgg cggtgttcct tcgtcgttgt
     2221 gcgggttgcc ggtgccgttg ttgtctgtcc acagctcgac ctcggtgatg ttcatcacct
     2281 tgttgtcctc gctgttctcg accttccagg tcaccgtcca ttgcccctgg gcgttcacac
     2341 acgacgtggt cgctgtgagt gtcgtgtgat gcgcggcggc cacgccggcg gtcagcgcaa
     2401 ggcccccagc gccgaccagc gcagcgaggc cgccgcggat cacgaacttg ccgacgcgaa
     2461 tgcgacgcga gctttgggaa ttcgacacga ctgacgccct cctgggctta cgaaagcttc
     2521 gtcctctttg acaaagtcac cgtaaagaag gcaatcgcac cgggcatcgt cgcaattgat
     2581 gatcgaacgc gacatgcgac ccgtcgcgaa cgacgcgacg aggacgcgac gaggggccga
     2641 ccaaaggccg gcccctcgta acccccacga tcccgaagga ccgtcggcgt gcgtgtccgc
     2701 gcgggtggtg ggttgggaca cgcgattgtc aggatgcgag tcgggttcgc ggcgcgcatc
     2761 ctcagaattg acgaacggga tcgcgcggtt actgctcgcg cgacgcgttc tcgagcgcgg
     2821 cgagcacctc gtgcaagcgg aagcgccggt gaccgcccaa ggtgcggaac gagggcagtc
     2881 gccccgactc cgcccagcgg gccactgtct tgcggtcgac gttggcgagt gccgccacct
     2941 cggccggtcg aaggagcgtt
//
LOCUS       DASUGA010000745         2023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1748036, whole genome shotgun sequence.
ACCESSION   DASUGA010000745 DASUGA010000000
VERSION     DASUGA010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748036"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VFX21_14690"
     CDS             complement(<1..623)
                     /locus_tag="VFX21_14690"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX5097271.1"
                     /translation="MRRAGRLAALAFVLASAAPAAAQTAPTSAPVDARYREFGDAGGF
                     RVTTAGTPAYDALITSPTDMAPEQVGDYYRDNSFGIAPTDVDRSYKPHPDVTIVRDRS
                     NGVPHIFGRTRYATVYAEGYATAEDRLFAMDVLRHYGRGRVSELLGLAEGEALDRAQV
                     ASTPYTEADLDDQLAQLQSERGGASIAEDLAAYADGVNAYIEEVTADP"
     gene            complement(634..990)
                     /locus_tag="VFX21_14695"
     CDS             complement(634..990)
                     /locus_tag="VFX21_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6285 domain-containing protein"
                     /protein_id="HEX5097272.1"
                     /translation="MTQDRPTAAELVAAVREFLERDVMEATEGRVQFHARVAVNALGM
                     VERELEQSAAMAAAERVRAAAILGHDGDARDLERELAAAIRSGAVDGEDGAVRAHVRA
                     TVREKLLVANPKYTQG"
     gene            complement(990..1976)
                     /locus_tag="VFX21_14700"
     CDS             complement(990..1976)
                     /locus_tag="VFX21_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX5097273.1"
                     /translation="MAKLDPDALNQVAGQAVGSPARVEGVRRLSGGASRETWSFDAVG
                     PGGELQPLVLRRDPGAHSGQSDRSTEYTLLRAAAAAGVPVPGVRYLLTDADGLGHGFV
                     MDRVEGETIARRILRDDEYAKARPLMAAQCGEIAARIHAVPRAALPDLPVQGAREQIA
                     QYRDTLDLFGEPHPAFELGLQWLEARAPTARADVVLVHGDFRNGNFIVGPEGIRAVLD
                     WELAHLGDPVEDLGWLCVKSWRFGHTEQLVGGFGDVDELLDAYAAAGGREVSPDELRY
                     WIAFGTLKWGVICIGQAFTHLNGLVRSVELATLGRRVAEMEWDLLDILDGGW"
BASE COUNT          280 a          741 c          701 g          301 t
ORIGIN      
        1 ggatccgccg tgacctcttc gatataggcg ttcacgccgt ctgcgtacgc ggcgagatcc
       61 tccgcaatcg acgcgccgcc gcgctccgac tgcaactgcg ccagttggtc gtcgagatcg
      121 gcttcggtgt acggcgtcga cgcgacctgg gcccggtcga gcgcttcgcc ctcggccagg
      181 ccgagtaact ccgacacgcg gccgcgcccg tagtggcgca gcacatccat cgcgaacaac
      241 cggtcctcgg cggttgcgta cccctcggcg tagacggtcg cgtaacgcgt gcggccgaag
      301 atgtgcggta cgccgttcga gcggtcgcgc acgatggtga catcggggtg cggcttgtac
      361 gaacggtcga cgtcggtggg cgcgatgccg aaggagttgt cgcggtagta gtcgccgact
      421 tgttcgggcg ccatgtcggt gggcgacgtg atgagcgcgt cgtacgcggg cgtgcccgcg
      481 gtggtcacac gaaagccgcc ggcgtcgccg aactcgcggt agcgcgcgtc gaccggcgcc
      541 gatgtcggcg ccgtctgggc cgcagctggt gccgcgctcg cgagcacgaa cgccaacgcc
      601 gccagccgac ccgctcgtcg catccgttgg tccctatccc tgcgtgtact tcgggtttgc
      661 gaccagcagc ttctcccgca cggtcgcgcg gacgtgcgcg cgcacggcgc cgtcctcgcc
      721 gtcgaccgcg cccgaccgga tggcggcggc cagttcgcgt tcgagatcgc gcgcgtcgcc
      781 gtcgtgaccg agaatcgcgg cggcccgtac tcgctccgcg gcagccatcg cggccgactg
      841 ttcgagctcg cgttcgacca tgccgagcgc gttcaccgca acgcgcgcgt ggaactgcac
      901 acggccctcg gtcgcctcca tcacgtcccg ttcgaggaac tcgcgcacgg ccgcgaccag
      961 ttcggccgcg gtcggacggt cttgggtcat caccagcctc cgtcgaggat gtcgagcaag
     1021 tcccattcca tctcggcgac gcgccgcccg agcgtcgcga gctcgaccga ccgcaccaag
     1081 ccgttgaggt gggtgaacgc ttgcccgatg cagatgacgc cccacttcag cgtgccgaac
     1141 gcgatccagt agcgcagttc gtcggggctc acctcgcggc cgccggccgc ggcgtacgcg
     1201 tcgaggagct cgtcgacgtc tccgaacccg ccgacgagct gctcggtgtg gccgaaacgc
     1261 cacgacttca cgcacaacca cccgaggtcc tcgaccgggt cgccgaggtg cgcgagctcc
     1321 cagtcgagca ccgcgcggat cccctcgggg ccgacgatga agttgccgtt gcggaagtcg
     1381 ccgtgcacca gcacgacgtc ggcgcgcgcc gtcggtgcgc gcgcttcgag ccactgcaat
     1441 ccgagctcga acgcaggatg cggttcgccg aagaggtcga gcgtgtcgcg gtactgcgcg
     1501 atctgttcgc gcgcgccttg cacgggcagg tcggggagcg cggcgcgcgg caccgcatgg
     1561 atgcgcgccg cgatctcgcc gcactgcgcg gccatgagtg gacgcgcctt cgcgtactcg
     1621 tcgtcgcgca ggatgcggcg cgcgatcgtc tcgccctcga cccggtccat gacgaagccg
     1681 tgcccgagcc cgtcggcgtc ggtcaggagg tagcgcacgc cggggaccgg cacgccggcc
     1741 gcggccgccg cccgcagcag ggtgtattcg gtcgaacggt cgctttggcc ggagtgtgca
     1801 cccgggtcac gccgcagcac cagcggctgc agctccccgc ccggcccgac cgcgtcgaac
     1861 gaccacgtct cgcgcgacgc accgcccgag agccgccgca caccttccac tcgcgccggg
     1921 gatccgaccg cctggcccgc gacctggttg agcgcatcgg gatcgagctt cgccaccggg
     1981 gcggaagtta ctgctgcgcc cgggccgggc ggcatctaca cac
//
LOCUS       DASUGA010000746         2980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_914764, whole genome shotgun sequence.
ACCESSION   DASUGA010000746 DASUGA010000000
VERSION     DASUGA010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914764"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..1735
                     /locus_tag="VFX21_14705"
     CDS             272..1735
                     /locus_tag="VFX21_14705"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5097274.1"
                     /translation="MTRLRWLTVLCVFALVAGACSRDKESTDAGDTNTPTSGSTETSG
                     GGDADAAGLDQGGFGDLGVLCSEAPADEPNAATGDVGVTADSIQVSTFSDPGFSGRLG
                     LNQELFDTADAFTSWCNEHGGIHGRQIELKKRDAKLTEFQQRVIEACDEGDFMMIGGG
                     NVLDDTGQTDRLGCATGAVPQIPGYVVTPQAAESDLTQQPVPNTGDEQPVGGFNYLAE
                     KFPDTKDAIGIFTGDIQTTITVADRNKEALQILGGYNIVYEGKYNPLGETSWRTYIEA
                     MRSAGVKGLVYVGEPSNLAAFLTEAQSVGMTFDWVWSAANIYDPAIENEAGAAADNLY
                     VTTGFYPFGGDLAKGNEATQQYLDLIDQYSDNGKVANLGLQAMSAWLLFAKATGECGA
                     DVTRDCVWEKAKAIKEWTGGGLNAPQDLSAGTASDCGALLDVKGGKFELTDIGANEGI
                     YLCDPANIVTLKNDYGSGVTCPNPAFADDPKPSNCAG"
     gene            1799..>2980
                     /locus_tag="VFX21_14710"
     CDS             1799..>2980
                     /locus_tag="VFX21_14710"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5097275.1"
                     /translation="MTEFLSFTVAGLSLAAIIAVGASGLVLTYTTTGVFNFAHGALGM
                     LGAFAYWQLRFDWGWPTPVAVVVVLLVLAPLAGMFLEVTIFRQLQHTTETIKLVVTVS
                     LLFSMIGIANWIWAPQSRFATRFYFGKGFEVLGTRVAYHELITVLVAIAVAIGLRFLL
                     YGTRSGISMRAAVDDRALSSLHGARPNRISMLAWAIGTSLAALSGILFVGTISLDATA
                     ISLLIVNAYAAAMIGRLRSLPLTFLGALILGLADGYLKGYLPKLNNQYLGSEFTSAVP
                     VIVLFVVLLLLPSARLRAAGTLRSREIIPVPTARGTAIFAAAIIAFAALALPLTTRSN
                     TITLSRVFAVGIVALSLFPLVGLAGQVSLCQWSLAGVGAVMMAHMGTNGSAIGVIAAI
                     AV"
BASE COUNT          492 a         1011 c          987 g          490 t
ORIGIN      
        1 cgccgacctc ctccgcgagc caggccacgg tggcgggcac gccccgcgcg cgatcgcgct
       61 cgccgtaggt gccggccatc agatcttgga actgcgagat gtccatccgg ccggtctacc
      121 aggggaaata cgcgacgcga ccacaaacgc gcaggccgcg gtgccacact ccctggatgc
      181 ttgcgtcgta cggccctatc agggcagtct cacgcggcta tccgacgggc attcgacggt
      241 ccaacacgcc gcgggcgggc gagggggaac aatgacgaga ctccgctggc tgacggtgct
      301 gtgcgtgttc gcgctggtcg cgggcgcatg cagccgggac aaggagtcga ccgacgcggg
      361 cgacaccaac accccgacgt ccggctcgac cgagacgagc ggcggcggtg acgccgacgc
      421 cgccggtctc gaccagggcg ggttcggtga tctgggtgtg ctgtgcagcg aggcgcccgc
      481 agacgagccc aatgcggcca ccggcgacgt cggggtgacc gcggactcga tccaggtcag
      541 cacgttctcc gacccgggct tctccggtcg cctcggtctc aaccaagagc tgttcgacac
      601 cgccgacgcg ttcacgagct ggtgcaacga gcacggcggg atccacggcc gccagatcga
      661 gctgaagaag cgcgacgcga agctgaccga attccaacag cgcgtcatcg aagcgtgcga
      721 cgaaggcgac ttcatgatga tcggcggcgg caacgtgctc gacgacacgg gccagaccga
      781 ccgcctcggc tgcgcgacgg gcgccgtccc gcagatcccc gggtacgtcg tcacgccgca
      841 ggccgcggaa tccgacctga cacagcagcc cgtgcccaac accggtgacg agcagccggt
      901 cggtgggttc aactacctcg ccgagaaatt ccccgacacc aaggacgcga tcggcatctt
      961 caccggcgac atccagacga cgatcaccgt cgccgaccgc aacaaggaag cgctccagat
     1021 cctcggcggc tacaacatcg tctacgaagg caagtacaac ccgcttggtg agacgagctg
     1081 gcgtacgtac atcgaggcga tgcgcagcgc gggtgtgaag ggcctcgtgt atgtcggcga
     1141 gccgtcgaac ctggccgcgt tcctgaccga ggcgcagagc gtcggcatga cgttcgactg
     1201 ggtgtggagc gcggcgaaca tctacgaccc cgcaatcgag aacgaagcgg gcgccgcggc
     1261 cgacaacctc tatgtcacga ccgggttcta tccgttcggt ggcgacctcg cgaagggcaa
     1321 cgaggcaacg cagcagtacc tcgacctgat cgaccagtac agcgacaacg gcaaggtcgc
     1381 gaacctcggt ctgcaggcca tgtctgcatg gctcctcttc gcgaaggcaa ccggtgagtg
     1441 cggtgccgac gtcacgcgcg actgcgtgtg ggagaaggcc aaggcgatca aggagtggac
     1501 cggcggcggc ctgaacgcgc cgcaagacct gagcgccggc acggcgtcgg actgtggcgc
     1561 gctcctcgat gtcaagggcg gcaagttcga actgacggac atcggggcca acgaaggcat
     1621 ctacctctgt gatccggcga acatcgtcac gctgaagaac gactacggct cgggtgtcac
     1681 gtgcccgaac ccggcgttcg cggatgaccc caaaccgtcc aactgcgcgg gttgacacgg
     1741 gcgtaggttt tcgtccgggc ccggtcgcgc gaccgggccc acccgtctcg gagtcgaatt
     1801 gacagagttc ctcagcttca cggtcgccgg gctgtccctt gcggcgatca tcgcggtggg
     1861 cgcgagcggc ctcgtgctca cgtacacgac cacgggcgtc ttcaacttcg cgcacggtgc
     1921 gctcggcatg ctcggcgcgt tcgcgtactg gcagctccgc ttcgactggg gctggccgac
     1981 cccggtcgcc gttgtcgtcg tgttgctggt gctcgccccg ctggcgggca tgttcctcga
     2041 agtcacgatc ttccgacagc tgcaacacac gaccgagacg atcaagctcg tcgtcaccgt
     2101 gtcgctgctg ttctcgatga tcggtatcgc gaactggatc tgggcaccgc agtcgcgctt
     2161 cgcgacgcgc ttctacttcg gcaagggttt cgaagttctc ggcacccgcg tcgcgtacca
     2221 cgagctcatc acggtgcttg tcgcgatcgc ggtggcgatc gggctgcgct tcctgctcta
     2281 cggcactcgc tcgggcatct cgatgcgggc cgcggtcgac gaccgggcgc tgtcgtccct
     2341 gcacggtgca cgacccaatc gcatctcgat gctggcgtgg gccatcggca cgtcactcgc
     2401 ggcgttgagc ggcattctct tcgtcgggac gatctccctc gacgcgaccg cgatctcgct
     2461 gctgatcgtg aacgcgtacg cggccgcgat gatcggtcgg ctgcgcagcc tcccactgac
     2521 gttcctcggc gcgctgatcc tggggcttgc cgacggctac ttgaagggct acctgccgaa
     2581 gctgaacaac caatacctcg gatccgagtt cacgagcgcg gtgccggtca tcgtcttgtt
     2641 cgtcgtgctg ctcctgttgc ccagcgcgcg tttgcgggcc gcgggcacgt tgcgcagccg
     2701 ggagatcatc ccggttccca ccgcgcgcgg cacggccatc ttcgccgccg cgatcatcgc
     2761 gttcgccgcg ctcgcgctgc cgctgacgac gcggtcgaac acgatcacgc tttcacgggt
     2821 gttcgccgtc ggtatcgtcg ccctgtcgct gttcccgctc gtcgggctcg cgggacaggt
     2881 gtctctctgt caatggagcc tcgcgggcgt cggcgccgtg atgatggcgc acatgggcac
     2941 caacggcagc gcgatcggcg tcatcgcggc gatcgcggtc
//
LOCUS       DASUGA010000747         3825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_498925, whole genome shotgun sequence.
ACCESSION   DASUGA010000747 DASUGA010000000
VERSION     DASUGA010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498925"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VFX21_14715"
     CDS             <1..248
                     /locus_tag="VFX21_14715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEX5097276.1"
                     /translation="TTTSTAVAGETTTIPQDTTTTVGPEGSTSTTVAPTSETSSGSET
                     GSDSLPFTGSGSGVLVLLGSGLVALGVLLSRRRYSLR"
     gene            367..531
                     /locus_tag="VFX21_14720"
     CDS             367..531
                     /locus_tag="VFX21_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097277.1"
                     /translation="MAVWVLGAFVVLLAVPAVAAQPDSSTLVLLAGAMITAGVLVLTT
                     DAQRHRSNSK"
     gene            complement(562..1488)
                     /locus_tag="VFX21_14725"
     CDS             complement(562..1488)
                     /locus_tag="VFX21_14725"
                     /inference="COORDINATES: protein motif:HMM:NF005861.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5097278.1"
                     /translation="MGLFDGKVAIITGAGRGIGRSHSLLLASEGAAVVVNDLGGGAAG
                     DGSDATPAQQVVDEITAAGGRAVANYDSVSSWAGAEKLIAQAVDAFGGLDVLINNAGI
                     LRDKMSFNMDEAEWDQVVDVHLKGHFATSKFAASYWRSKSKETGEAVNGKIVNTASES
                     GLYGNAGQVNYAAAKAGIASMTIVMARELERIGVRVNAICPVARTRLTETVAGAGEFM
                     KAKEGEFDRFAPENISAVAGWLASDLSAGVTGQVFKVMGGQVQLLRGWRPVTEATDDK
                     PWTIDSIESNATALFGGIDRGVPPFMPNVGDA"
     gene            complement(1578..2009)
                     /locus_tag="VFX21_14730"
     CDS             complement(1578..2009)
                     /locus_tag="VFX21_14730"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5097279.1"
                     /translation="MKQRQRVAAYNVVVDEHSRVLLVRLSDITEAPGHWTLPGGGIEF
                     GEHPEAAAIRELHEETGLRGRILELLTVDAVHRVAREAGEDFDYHAIRIVYRTEIESA
                     DLVHEADGSTDMAEWFTFEELDEISLVPIAALGSRFAFGDR"
     gene            complement(2006..2806)
                     /locus_tag="VFX21_14735"
     CDS             complement(2006..2806)
                     /locus_tag="VFX21_14735"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5097280.1"
                     /translation="MELRIEKDVDGVGRIVLDRPEAKNALTIAMRDGITEAVRDFRAD
                     PKVRAVLVTGEGTAFCAGMDLSASTLAQAGKPGFNPRSTSEALRVGVQTFIRELWQLD
                     KPTVAAVNGAAVGPGAHLALACDFVFVSDRTKFMWSFAKWGLVADAGGAYLLPRLVGM
                     ARAKAMLMLGETTTGSDAVAQGLAYRCVESDDALVPEALALTQQLAAGPTRSLGLSKQ
                     LLNWSFETDLGQCLESESHFQALAATSPDVVEGMTAFREKRDAKFTGA"
     gene            complement(2809..>3825)
                     /locus_tag="VFX21_14740"
     CDS             complement(2809..>3825)
                     /locus_tag="VFX21_14740"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097281.1"
                     /translation="ATQRGTLWGQEGIGGNTIPTAIGLTGIPCTRVENACPSGSDAFR
                     VGAMAVASGVHDVVLVIGVEKMRDKSSEEGLLSRAAAGHPIFTRGETAPVLFAPFATR
                     HMHEYGTTREMLASVAVKNHYNGARDPYAHFQNEITVDDVLKSAPVCHPLHLLDCCPQ
                     TDGAAAALLVSADRAREFTDKPVYVAGFGVATDHPYLHEKDSFTEIKATRLASQRAYA
                     MAGIEPNQVDAAEVHDCFTITEILDIEDLGFFEKGDGGRASLEGETSLTGRIPVNTSG
                     GLLAKGHPIGATGVAQITECWWQLRGDAGDRQVEIRNGYALQHNAGGRGSGVSVVNIL
                     TNKA"
BASE COUNT          589 a         1305 c         1275 g          656 t
ORIGIN      
        1 cgacgacgac gagcaccgcg gtcgccggtg aaacgacaac gattccgcag gacacgacga
       61 ccacggtcgg acccgagggc tcgacgtcga ccacggtcgc accgacgtcg gagacgagtt
      121 ccgggtcgga gaccggcagc gactcgctgc ccttcaccgg cagcggttca ggcgtgctgg
      181 tcctgttggg ttcgggcctc gttgcgctcg gtgtgctgtt gagccggcgc cgttattcgc
      241 tgcggtaatc gaatgctccg cttctcctgc ccttcgggcg ccgttggcgt ccgagggcgg
      301 cggagtggat caaggcaaga cagcagggcg tggggataag gggggtccca cgtgacacga
      361 tcgacattgg cagtctgggt tctcggcgca ttcgtcgtgc tgctcgcagt tcccgccgtc
      421 gcggcccaac ccgatagctc gacgctggtg ttactcgccg gtgcgatgat caccgccggc
      481 gtactggtgc tcaccacgga cgcgcaacgg caccgcagca attccaagta acgcagcgga
      541 gtgcgcgacc aacgcgcgtg tctatgcgtc gccgacgttg ggcatgaagg gcggaacgcc
      601 gcggtcgatg ccgccgaaca gggcggtggc attggattcg atcgagtcga tcgtccaggg
      661 cttgtcgtcg gtggcttcgg tcaccgggcg ccagccgcgc agcagctgca cctggccgcc
      721 catcaccttg aacacctggc cggtcacgcc cgccgacagg tccgacgcca gccagccggc
      781 gacggcggaa atgttctcgg gagcgaagcg gtcgaactcg ccttccttcg ccttcatgaa
      841 ctcgcccgcg cccgcgacgg tctcggtcag gcgagtgcgc gcaacggggc agatcgcgtt
      901 gacgcgcacc ccgatgcgtt cgagttcgcg tgccatcaca atcgtcatcg acgcgatgcc
      961 ggccttcgct gccgcgtagt tgacctggcc ggcgttgccg tagaggcccg actccgacgc
     1021 ggtgttcacg atcttgccgt tgaccgcctc gccggtctcc ttcgacttcg accgccagta
     1081 cgaggcggcg aacttcgagg tggcgaagtg acccttcaag tggacgtcga cgacctggtc
     1141 ccactcggcc tcgtccatgt tgaagctcat cttgtcgcgc aggatgccgg cgttgttgat
     1201 gagcacgtcc aagccgccga acgcgtcgac cgcctgcgcg atgagctttt cggcgccggc
     1261 ccaggatgaa acgctgtcgt agttcgcgac ggcgcggccg ccggccgcgg tgatctcgtc
     1321 gacgacctgc tgcgcgggcg tggcgtcgga gccatctccg gctgcgccgc cgccgaggtc
     1381 gttgacgacg accgccgcgc cttccgacgc gagcaacagc gagtggctgc ggccgatgcc
     1441 gcgccctgcg ccggtgatga tcgcaacctt gccgtcgaac agacccacga atgcctcccg
     1501 gttcctgcgg ccacgctgac gccgatgtca ggtgggcggg gcgtcaggtt aggaggcggc
     1561 ctcgcgaacc ggcaatgtca gcggtcgccg aatgcgaaac gggaaccgag cgccgcgatg
     1621 ggcacgagcg agatttcgtc gagctcctcg aaggtgaacc actccgccat gtcggtcgag
     1681 ccgtcggctt catggacgag gtcggccgac tcaatttcgg tgcggtagac gatccggatc
     1741 gcgtggtagt cgaaatcctc gcccgcttcg cgggcgaccc ggtgcaccgc gtcgaccgtc
     1801 agcagttcga ggatgcggcc gcgcagtccg gtctcttcgt ggagctcgcg gatcgcggcg
     1861 gcttcggggt gctcgccgaa ctcgatgccg ccccccggca gtgtccagtg cccgggcgct
     1921 tcagtgatgt ccgacagtcg aaccagcagc acccggctgt gttcatcgac cacgacgttg
     1981 tacgccgcga cccgctgccg ctgtttcacg cgccggtgaa cttggcgtcg cgcttctcgc
     2041 ggaaggcggt catgccttcg acgacatccg gcgacgtcgc cgcgagcgct tggaagtgcg
     2101 actcgctttc gagacactgc ccgaggtccg tttcgaacga ccaattcagc agctgcttcg
     2161 acagtccgag cgagcgcgtc ggtcccgccg ccagctgctg cgtgagcgcg agcgcctccg
     2221 gcaccaacgc gtcgtccgac tcgacgcagc ggtaggcgag gccctgcgcg accgcgtccg
     2281 aacccgtcgt cgtttcaccg agcatgagca tggccttggc gcgcgccatc ccgaccaagc
     2341 ggggaagcag gtacgcgccg cctgcgtcgg cgacgaggcc ccatttcgcg aacgaccaca
     2401 tgaacttcgt gcggtcactg acgaacacga agtcgcatgc cagcgcgagg tgcgcgcccg
     2461 gtccgactgc ggcgccgttg acggccgcga cggtcggctt gtcgagctgc cagagttcgc
     2521 gaatgaacgt ctgcacgccg acccgcagcg cttcggacgt cgaacgcgga ttgaacccgg
     2581 gcttgcccgc ctgcgcgagc gtcgacgccg agaggtccat gccggcgcag aacgcggtgc
     2641 cctcgcccgt gacaaggaca gcgcgcacct tgggatcggc gcggaagtcg cgcaccgcct
     2701 cggtgatgcc gtcgcgcatc gcgatcgtca acgcgttctt tgcttcgggc cggtcgagca
     2761 cgatgcggcc gacgccgtcg acgtctttct cgatccggag ttccatgttc aggccttgtt
     2821 cgtcaggatg ttcacgaccg acacgcccga cccgcggccg cccgcgttgt gttgcaacgc
     2881 gtagccgttg cggatctcca cctggcggtc accggcatcg ccccgcagtt gccaccagca
     2941 ctcggtgatc tgcgcaacgc cggttgcgcc gatcgggtgg cctttcgcga gcaatccgcc
     3001 gctcgtgttc accgggatgc gacccgtcag cgaggtctcg ccttcgaggc tggcgcgccc
     3061 gccgtcgccc ttttcgaaga agccgaggtc ttcgatgtcg aggatctcgg tgatcgtgaa
     3121 gcagtcgtgc acctcggctg cgtcgacctg gttcggttcg atgcccgcca tcgcgtacgc
     3181 gcgctgggac gcaagccgcg tcgccttgat ctcggtgaag ctgtctttct cgtgcaggta
     3241 ggggtggtcg gtcgcgaccc cgaaccccgc cacgtagacc ggcttatcgg tgaattcccg
     3301 agcgcgatcg gcactgacca acagcgcggc ggcggcacca tcggtctgcg ggcaacagtc
     3361 gagcagatgc agcggatggc acaccggcgc cgacttcaac acatcgtcga ccgtgatctc
     3421 gttctgaaaa tgcgcatacg gatcgcgcgc gccgttgtag tggttcttca ccgcgaccga
     3481 cgcgagcatc tccctggtcg tgccgtactc gtgcatgtgg cgcgtggcga acggcgcgaa
     3541 cagcacggga gcggtttcgc cgcgtgtgaa aatcgggtgt ccggccgcgg cgcgcgacag
     3601 gagcccttcc tccgacgact tgtcgcgcat cttctccacg ccgatgacga gcacgacatc
     3661 atgaaccccg ctcgcgacgg ccatcgcgcc gacccggaac gcgtccgagc ccgacgggca
     3721 ggcgttctcg acgcgcgtgc acggaatacc ggtgaggcca atggccgtcg ggatcgtgtt
     3781 gccaccgatg ccctcctggc cccacaaggt gcctcgctgc gtggc
//
LOCUS       DASUGA010000748         3838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_915669, whole genome shotgun sequence.
ACCESSION   DASUGA010000748 DASUGA010000000
VERSION     DASUGA010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915669"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VFX21_14745"
     CDS             complement(<1..308)
                     /locus_tag="VFX21_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008741050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEX5097282.1"
                     /translation="MGDLVRLCARADLDPGTARRFDIDGHRIALVRIGDDFYAIGDEC
                     SHADYSLSEGDVWEEECEIECPKHGSTFLLTTGEPQTLPATQPVPVYDIQLDGDDVKV
                     V"
     gene            complement(301..1593)
                     /gene="sufD"
                     /locus_tag="VFX21_14750"
     CDS             complement(301..1593)
                     /gene="sufD"
                     /locus_tag="VFX21_14750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX5097283.1"
                     /translation="MTAAFTPDAARAVGGPKWLIDRRAAAAEQLAGITWPDATEEIWR
                     YSRIGELDLARYTPFAEAELGVPGDERAPGGGPWAAEAGKRAGMLVVRDGHVVRHEID
                     PSLEAKGVRVCDLATCDEPEIRDLLGRVSGATPDAFTTLHDAFLSGGAFIQVPPGVVV
                     EDPILVLHWCEGDGRASFPHTLVHVGESAEVTVLDRFGSPATDHLVDAVTELIVEDNG
                     HLRYLSVQEHGPRTWHLGFQRADLGRDASVRTSAVALGGDYARLRSEACLGGQGSESD
                     QLAVYFGDGSQMLDFRTLQDHDAPSTRSDLLFKGAVEDTARSVYSGLVRLRRPAQKSN
                     AFQTNRNLVLTEGASAESIPNLEIEANDVRCSHASTVGPIDEDQLYYLESRGIQPEEA
                     ERLIVLGFFDDVFERLPVGALTTGLRRSVVDKLEHRRG"
     gene            complement(1599..3002)
                     /gene="sufB"
                     /locus_tag="VFX21_14755"
     CDS             complement(1599..3002)
                     /gene="sufB"
                     /locus_tag="VFX21_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX5097284.1"
                     /translation="MAVSGIDLGKYKLGWHDTESYAITPKKGLNEQVVKDISWHKSEP
                     EWMTKFRLNSLKRFERKPMLEWFAKNMPTIDFDDIYYYLKPQQEGAVSDWDMLPDEMK
                     ATYEKLGIPEAERKFLAGVTSQYDSEVVYHRNREDLEKKGILFTSMDQALIDYPEIVR
                     EHFGTVIPPGDNKFAALNSAVWSGGSFIYVPPGVHVDMPLQAYFRINGENAGQFERTL
                     IIADEGASVHYVEGCSAPVFSTDSLHSAVVELIAKPGARIRYTTIQNWSPNVYNLVTK
                     RARAETEATVEWIDGNLGSKLTMKYPAVVMTGPKAHGEVLSVAYAGPGQHQDAGAKMT
                     HAAPETTSIIDSKSISKDGGRTTYRGLVRVEEGAHNVKSHVRCDALILDDQSRSDTYP
                     YMEIEEKDARIGHEATVSKVGDDQLFYLQSRGLTEQQATAMIVNGFIEPITKTLPMEY
                     AVELSRLIELNMEGAVG"
     gene            complement(3102..>3838)
                     /locus_tag="VFX21_14760"
     CDS             complement(3102..>3838)
                     /locus_tag="VFX21_14760"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX5097285.1"
                     /translation="VLQRSFTPRTKGGVLSAFVMARSDPENYGKIILYDTPDSTSSPS
                     PAQAATSIEADQFISSRFTLLDQRSSNVERGQVQLLPIGDTIVYIRPIWVTGGSSAQT
                     FPRYNFIAATVGERAKLGYSVQDAVTALVTGGQTDYEKDVAAGRSTPGVTPADGNNNN
                     GATTTTTTPTTPGTQPPGDLTVAQLVDRAVAEFNEADAARKSGDIVGYIQHVQAAQAD
                     IAAAQAKAKATPSAATTTTVPPSASP"
BASE COUNT          607 a         1254 c         1274 g          703 t
ORIGIN      
        1 acaaccttca cgtcgtcgcc gtcgagctgg atgtcgtaca ccggcaccgg ctgcgtcgcg
       61 ggcagcgtct gcggttcgcc ggtcgtgagg aggaacgtgg agccgtgctt ggggcactcg
      121 atctcgcatt cctcttccca cacgtcgcct tcggacaagg agtagtcggc gtgcgagcac
      181 tcgtcgccga tggcatagaa gtcgtcaccg atacgcacca acgcaatgcg gtgaccgtcg
      241 atgtcgaagc ggcgcgcggt gccgggatcg agatcggcgc gcgcacagag ccggacgaga
      301 tcacccacgg cggtgctcca gcttgtcgac gaccgaccgg cggaggccgg tcgtgagcgc
      361 gccgacagga agccgctcga acacgtcgtc gaagaagccg agcacgatga ggcgctcggc
      421 ctcctccggc tggatgccgc gcgattcgag gtaatagagc tggtcttcgt cgatgggccc
      481 gacggtcgac gcgtgcgagc accgcacgtc gttcgcttcg atctcgaggt tcgggatcga
      541 ttccgcgctc gcgccctcgg tcagcacgag atttcggttc gtctggaacg cattcgattt
      601 ctgggccggg cggcgcagcc gcacgagccc cgagtatacg gagcgggcgg tgtcctcgac
      661 cgcgcccttg aagaggaggt cgctgcgcgt cgacggcgcg tcgtgatcct gcagcgtgcg
      721 gaaatcgagc atctgactgc catcgccgaa gtacacggcg agctggtcgc tctcactccc
      781 ctgcccgcca aggcacgctt cgctgcgcag gcgcgcgtaa tcgccgccga gcgcgactgc
      841 cgacgtgcgc acgctcgcgt cgcggccgag gtcggcgcgc tggaaaccga ggtgccacgt
      901 gcgaggcccg tgctcctgca ccgacagata gcgcaggtgg ccgttgtcct ccacgatcag
      961 ttcggtcacg gcatcgacga ggtggtctgt cgccggcgac ccgaaccggt cgagcacggt
     1021 cacttccgcg ctttcgccga cgtgaaccag cgtgtgcggg aatgacgcgc ggccgtcgcc
     1081 ttcacaccaa tgcaacacga ggatcgggtc ttccacgacg acacccggcg gcacctgaat
     1141 gaacgcgcca ccggaaagga acgcgtcgtg gagcgtggtg aacgcgtcgg gcgtcgcgcc
     1201 ggagacgcga ccgagcaggt cgcgaatctc cggttcgtcg cacgtcgcga gatcgcagac
     1261 acgcacaccc ttggcttcca acgaaggatc gatttcgtgc cgcacgacgt gcccgtcgcg
     1321 tacgacgagc atgcccgcgc gcttgccggc ttccgccgcc cacggaccgc ccccgggtgc
     1381 ccgctcgtcg ccgggcacac ccagctccgc ctccgcgaac ggcgtatagc gcgcgaggtc
     1441 gagttcgccg atgcgcgagt agcgccagat ctcttcggtc gcgtcgggcc aggtgatgcc
     1501 ggcgagttgc tcggccgcgg ccgcgcgccg gtcgatgagc cacttgggcc cgccgactgc
     1561 acgcgccgca tcaggagtga atgcggcagt catcgtcgtt acccgactgc gccttccatg
     1621 ttcagctcga tgagccggga aagctcgacc gcgtactcca tcggcagcgt cttcgtgatc
     1681 ggctcgatga acccgttgac gatcatcgcg gttgcctgct gctcggtgag tccgcggctc
     1741 tgcagataga agagctggtc gtcgccgacc ttcgagacgg tcgcctcgtg cccgatgcgc
     1801 gcgtccttct cctcgatctc catgtacggg taggtgtcgc tgcggctttg gtcgtcgagg
     1861 atcagcgcgt cgcagcgcac gtgactcttg acgttgtgcg caccttcctc gacgcgcaca
     1921 aggccgcggt acgtcgtgcg gccgccgtcc ttgctgatcg acttcgagtc gatgatcgac
     1981 gtcgtctcgg gcgcggcgtg cgtcatcttg gcccccgcgt cctggtgctg gccgggtccc
     2041 gcgtaggcaa ccgacagcac ttcgccgtgc gccttcggac cggtcatgac aactgccggg
     2101 tacttcatcg tgagcttcga cccgaggttg ccgtcgatcc actcgactgt cgcctcggtt
     2161 tcggcccggg cgcgcttggt caccaggttg tagacgttcg gcgaccagtt ctggatcgtc
     2221 gtgtaacgaa tacgtgcgcc cggcttcgca atcagctcca ccaccgcgga gtgcaacgag
     2281 tcggtcgaaa agaccggcgc cgagcagccc tcgacgtagt gcaccgacgc gccttcgtca
     2341 gcgatgatga gcgtgcgctc gaactgtccg gcgttctcac cgttgatgcg gaagtacgcc
     2401 tgcagcggca tgtcgacgtg cacacccggc ggtacgtaga tgaacgatcc gccggaccac
     2461 accgccgagt tgagcgcggc aaacttgttg tcaccgggcg ggatgacggt tccgaagtgc
     2521 tcgcgcacga tctccgggta gtcgatgagc gcttggtcca tcgaggtgaa gaggatgccc
     2581 ttcttctcga ggtcttcacg gttgcggtgg tacacgactt cggagtcgta ctgcgaagtc
     2641 acgccggcga ggaacttgcg ctccgcttcc ggaatgccga gcttctcgta cgtcgccttc
     2701 atctcgtcgg gaagcatgtc ccagtcggag acggcgcctt cttgctgcgg cttcaagtag
     2761 tagtagatgt cgtcgaagtc gatggtcggc atgttcttcg cgaaccactc gagcatgggc
     2821 ttgcgctcga agcgcttgag cgagttgagc cggaacttcg tcatccactc gggctcagac
     2881 ttgtgccaag agatgtcctt gacgacctgc tcgttgagcc ccttcttggg ggtgatcgcg
     2941 tagctctcgg tgtcgtgcca accgagcttg tacttgccca gatcgatgcc agagacggcc
     3001 atcagacacc cttcgggacg gcaattaaca ctacgtccat agtaacaatc ccgcggaggc
     3061 ccaccaagcc cggggcccgg cccgaagcga gatcggggag gctacggcga ggcgctgggc
     3121 ggaaccgtcg tcgtggtcgc cgccgatggt gtggccttgg ccttggcctg ggcggcggca
     3181 atgtcggcct gggcggcctg gacgtgttgg atgtacccga cgatgtcgcc gctcttgcgg
     3241 gcggcgtcgg cctcgttgaa ctccgcgacc gcccggtcga cgagctgcgc caccgtgaga
     3301 tcgccgggcg gctgggtgcc gggcgtggtc ggggtcgtcg tggtcgtcgt cgccccgttg
     3361 ttgttgttcc cgtcggccgg ggtcacgccc ggcgtcgaac ggcccgcggc aacgtccttc
     3421 tcgtaatccg tctgtccgcc ggtcaccagc gcggtgaccg catcctgcac cgagtagccg
     3481 agtttggcac gctcccccac cgtggccgcg atgaagttgt agcgcgggaa cgtctgcgcg
     3541 ctgcttccac ccgtgaccca gatcggacgg atgtacacga tcgtgtcgcc gatcggtaac
     3601 agctgtacct gaccgcgttc gacgttcgag gatcgctgat cgagcagcgt gaaacgactc
     3661 gagatgaact gatccgcttc gatcgaggtg gcagcctgcg cgggcgacgg cgacgacgtc
     3721 gagtcggggg tgtcgtagag gatgatcttc ccgtagttct ccggatcgga tcgcgccatc
     3781 acgaacgccg acagcacacc gcccttcgtg cgcggcgtaa acgaccgctg taagacga
//
LOCUS       DASUGA010000749         2360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_81081, whole genome shotgun sequence.
ACCESSION   DASUGA010000749 DASUGA010000000
VERSION     DASUGA010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81081"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1548)
                     /gene="lipA"
                     /locus_tag="VFX21_14765"
     CDS             complement(<1..1548)
                     /gene="lipA"
                     /locus_tag="VFX21_14765"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX5097286.1"
                     /translation="MSPTLRARWLGTVPYAEAEVLQRAIHEHSDEHYLLLQEHPHVYT
                     LGSSAKREHILRDPASVGAELIDADRGGDVTYHGPGQLVGYPVIGLGEWRAGQRDVVA
                     YVRRLESVLISVLADFGITATVSKGYTGVWVGDEKVAAIGVRVARGRTRHGFALNVDP
                     DLAMFEHIVPCGIRDRGVTSMARVLGAAPDMQAVVERVVARFAEQFGYGDVEFAAVSS
                     KRVVVDPCEQPVALLTKRPDWMKVRARFDDGYLELKKMMRGLELHTVCEEAGCPNIYE
                     CWSDRTATFMILGDRCTRACGFCQVDTRKPLAVDVNEPARVAEAVVTMGLAHAVITCV
                     ARDDLPDGGASVFAATVDAIRDASPDTAIELLIADNKGDAASLATIFDARPDVLNHNL
                     ETVARLQRVARPSAGYARSLAVLARAKAAGLLTKSGLIVGMGETEDELRAAITDLRAV
                     GVEILTIGQYLRPTGQHLPVARWWTPDEFERLGTFAEGLGFRHVESGPLVRSSYHARA
                     AADTLTVT"
     gene            complement(1545..>2360)
                     /locus_tag="VFX21_14770"
     CDS             complement(1545..>2360)
                     /locus_tag="VFX21_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX5097287.1"
                     /translation="VARAGGGAARTAPAPAAPVAPAAAPAAAPVSRGTGDEVVPFDNI
                     RRRTAEHMVRSKATSAHVYTSVRVDFERVEKARRAAQAEWKATEGFGLTYLPFIVRAF
                     SDVVHEYPNVNASVDGDSLVVHRDINLGIAVDLEFKGLIAPVIKNADGKRLRLIAREI
                     RDLAGRAKTKQLGPDEVVGGTFTITNMGPFGTTLTLPIINQPQVAILATDGIRKEPVV
                     IEGPDGEDTIAIHHVGHLALAWDHRAFDGAYAAAFLNEMRNVLESRDWDTELA"
BASE COUNT          359 a          834 c          807 g          360 t
ORIGIN      
        1 cgtgacggtg agcgtgtcgg ccgcggcgcg ggcgtggtag ctcgagcgca ccaacggacc
       61 gctctcgacg tgacggaagc cgaggccttc ggcgaacgtg cccaggcgct cgaactcgtc
      121 cggcgtccac caacgcgcaa ccggcaggtg ttgcccggtc gggcgcaaat actggccgat
      181 ggtcaggatc tcaacaccga cggcgcgcag gtcggtgatc gcggcgcgca actcgtcctc
      241 ggtctcgccc atgccgacga tcaaccccga cttcgtgagc agacccgcag ccttcgcgcg
      301 cgcgagcacc gcgagtgaac gcgcgtaccc ggcggacggc cgcgcaaccc gctgcaatcg
      361 cgcgaccgtt tcgaggttgt ggttcaacac gtcgggccgg gcgtcgaaga tcgtcgcgag
      421 cgacgccgcg tcgcccttgt tgtccgcgat gagtagctcg atcgcggtgt ccgggctcgc
      481 atcccggatg gcatcgaccg tcgccgcgaa cacggatgcg ccaccgtcgg gcaggtcgtc
      541 gcgcgcaaca cacgtgatga ccgcgtgcgc gaggcccatc gtcacgaccg cctcggccac
      601 ccgcgccggc tcgttcacgt cgaccgccaa cggcttgcgc gtgtcgacct ggcagaaccc
      661 gcacgcacgc gtgcagcggt cgccgaggat catgaacgtc gcggtgcggt ccgaccagca
      721 ctcgtagatg ttcgggcacc cggcctcttc acacacggtg tgcagctcga ggccgcgcat
      781 catcttcttg agctcgaggt agccgtcgtc gaagcgggcc cgcaccttca tccagtcggg
      841 ccgcttcgtg agcaacgcaa ccggttgctc gcaggggtcg acgacgacac gcttcgatga
      901 cacggcggca aactcgacgt cgccatagcc gaactgctct gcgaagcgag cgacgacgcg
      961 ctccacgacc gcctgcatgt cgggcgcggc acccaggacg cgcgccatcg acgtcacgcc
     1021 gcggtcacga ataccgcacg gcacgatgtg ctcgaacatc gccaggtcgg ggtcgacgtt
     1081 caacgcgaac ccgtgccgcg tgcgcccgcg cgcgacgcgc acgccgatcg ccgcgacctt
     1141 ctcgtcgccg acccacacgc cggtgtagcc cttcgacacg gtcgcggtga tgccgaagtc
     1201 ggcgagtacc gagatcaaca ccgactcgag ccggcgcacg tacgcgacga cgtcgcgctg
     1261 tccggcgcgc cactcgccga gtccgatgac cggatagccg acgagctggc cgggaccgtg
     1321 gtaggtgacg tcgccgcccc ggtcggcgtc gatcagttcg gcaccgaccg atgccgggtc
     1381 gcgcaggatg tgctcgcgtt tcgcggacga cccgagtgtg tagacgtgcg ggtgctcctg
     1441 cagcagcagg tagtgctcgt cactgtgctc gtggatcgcg cgctggagga cttccgcctc
     1501 cgcatacggg acggtgccca gccagcgagc gcgtagcgtc gggctcacgc cagctcggtg
     1561 tcccagtcgc gcgactcgag cacgttgcgc atctcgttga ggaacgccgc cgcatacgcg
     1621 ccgtcgaacg cgcggtggtc ccacgcgagc gcgaggtggc cgacgtggtg gatcgcgatc
     1681 gtgtcctcgc cgtcgggtcc ttcgatcacc accggttcct tgcggatgcc gtcggtggcg
     1741 aggatcgcga cctgcggctg gttgatgatc ggcagcgtca gggtggtgcc gaacggcccc
     1801 atgttcgtga tcgtgaacgt gccgccgacg acttcgtcgg gcccgagctg cttggtcttg
     1861 gcgcgccccg cgaggtcgcg gatctcacgc gcgatcaacc gcaggcgttt gccgtcggcg
     1921 ttcttgatca ccggcgcgat gagacccttg aactcgaggt cgacggcaat gccgaggttg
     1981 atatcgcggt gcaccaccag cgagtcgccg tcgacgctcg cgttcacgtt cgggtactcg
     2041 tgcacgacat cggagaaggc gcgcacgatg aacggcaagt aggtgaggcc gaagccctcc
     2101 gtcgccttcc attcggcctg cgcggcgcgg cgggccttct cgacgcgctc gaagtcgacg
     2161 cgcaccgacg tgtagacgtg cgcgctcgtc gccttcgacc gcaccatgtg ctcggccgtg
     2221 cggcggcgga tgttgtcgaa cggcacgacc tcgtcgcccg taccccgtga caccggcgcc
     2281 gcagcgggag ccgcggccgg tgccaccggt gccgcgggcg caggcgcggt acgcgccgcg
     2341 ccaccaccgg cgcgcgcgac
//
LOCUS       DASUGA010000750         2350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_289425, whole genome shotgun sequence.
ACCESSION   DASUGA010000750 DASUGA010000000
VERSION     DASUGA010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289425"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VFX21_14775"
     CDS             <1..595
                     /locus_tag="VFX21_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HEX5097288.1"
                     /translation="YEDDGRPLSGRSGKLTRLEEIVESVFAAGEKMLIFTHFAEWGIK
                     LAAHLAERTGVPTDCYHGGMTRTARDQVIDAFQNREGPAALVLSLKAGGTGLNLTAAS
                     HVVLYDRWWNPAVEDQARDRAWRLGQTRTVISHRLICPGTVDERVEEVVEGKRRIADL
                     VLPKSSSLADLDSSQLRVALGIRPDTVLTDDPSGARL"
     gene            592..894
                     /locus_tag="VFX21_14780"
     CDS             592..894
                     /locus_tag="VFX21_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097289.1"
                     /translation="MTPSRPRKGAKRPAARRRPATGRDFWGTDAIEEEPADTIRPIDE
                     PAAMVLSLGPPPLRGHEVAAEQYFRVVYDQAVALAVALAAANGLLDTEGDDDTPAG"
     gene            1005..1505
                     /locus_tag="VFX21_14785"
     CDS             1005..1505
                     /locus_tag="VFX21_14785"
                     /inference="COORDINATES: protein motif:HMM:NF022161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HEX5097290.1"
                     /translation="MEIQDAHVYPVPVDAVVAMLRDQAATIEKYESMGHRDVQILECE
                     ADDDALTIKSSRVVDVDLPGFAKKVLKPTNTMVQTDEWRRARDGSWAGTFDVEVKGAP
                     VRIAGTMRLAPADAGCRHEVTIDFQVKVPIVGGKIADWAAKNDGRRTLSAEFAFNDER
                     LRAHDA"
     gene            complement(1485..1643)
                     /locus_tag="VFX21_14790"
     CDS             complement(1485..1643)
                     /locus_tag="VFX21_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097291.1"
                     /translation="MGKTVSLASSDGQFAVTVAGDDVELVLSVLLLLEVSGSKEVTDA
                     RESRVVRA"
     gene            1860..>2350
                     /locus_tag="VFX21_14795"
     CDS             1860..>2350
                     /locus_tag="VFX21_14795"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX5097292.1"
                     /translation="MRPDDPALGPSEILIGCVNFEAVPRDKGATLEKMNAFVAEAAAR
                     DCDLVIFPELALNTWGKCAACAEAHRPCEWHRGQAESAAGPSCRAIVDMAAAHGVHVI
                     YGYEEAGDDDVTAIYNSANIVAPSGLVGTYRKLHLGIPLETDRFTPGDALPVFETALG
                     PIG"
BASE COUNT          404 a          794 c          803 g          349 t
ORIGIN      
        1 ctacgaagac gacggccgcc cgttgtccgg ccgctccggc aagctcacgc gactcgagga
       61 gatcgtcgag tcggtgttcg cggccggcga gaagatgctc atcttcacgc acttcgcgga
      121 gtggggcatc aagctcgccg cgcatcttgc ggagcgcacc ggtgtgccga ccgactgcta
      181 ccacggcggt atgacgcgca ccgcgcgcga ccaggtgatc gacgcgttcc agaaccgtga
      241 gggcccggcc gcactcgtgc tgtcgttgaa ggccggaggg acgggtttga acctcaccgc
      301 cgcgagtcac gtcgtgctct acgaccgctg gtggaacccg gccgtcgaag accaggcgcg
      361 cgatcgagcg tggcggctcg gtcagacccg caccgtcatc tcgcaccggc tcatctgtcc
      421 cggcacggtc gacgagcgtg tcgaggaagt cgtcgagggc aagcgccgca tcgccgactt
      481 ggtcctgccg aagtcgagct cgctcgccga cctcgacagc tcccagctgc gtgtcgcgtt
      541 gggtatccgt cccgacacgg tgttgaccga cgacccgagt ggcgcgcggc tgtgacaccg
      601 tcacgaccgc gcaagggagc gaagcgcccg gccgcgcgcc ggcgacccgc gaccggtcgc
      661 gacttctggg gcaccgacgc gatcgaggaa gaaccggccg acacgattcg cccgatcgac
      721 gaaccggcgg ccatggtgct gtcgctcggg cctccgccgc tgcgcgggca cgaagtcgcg
      781 gccgagcaat atttccgagt ggtgtacgac caagcggtcg cgctggccgt tgcccttgcc
      841 gccgcgaacg gtctgctcga caccgaggga gacgacgaca cccccgcggg gtgaacgcag
      901 acacgagtcc gttgacccgc gcgacggcgg cgagcgcatc ttgcccgcga cgagacgagt
      961 tggaaagctc cttctgcagg ccgttagcaa ctgcggaggt cgtcgtggag atccaggatg
     1021 cgcacgtcta tccggtcccg gtcgacgcgg tcgtcgcgat gttgcgcgac caggccgcga
     1081 ccatcgagaa gtacgagagc atgggtcacc gcgacgtgca gatactcgaa tgcgaggccg
     1141 acgacgatgc gctgacgatc aagtcgtcgc gcgtcgtcga cgtggacctc cccgggttcg
     1201 cgaagaaagt gttgaagccg acgaacacga tggtgcagac cgacgaatgg cggcgcgcgc
     1261 gcgacggcag ctgggccggc accttcgatg tcgaggtgaa gggcgcgccc gtccgcatcg
     1321 cgggtacgat gcgcctcgcg ccggccgacg ccgggtgccg ccacgaggtc acgatcgact
     1381 tccaggtgaa ggtgccgatc gtcggcggca agatcgccga ttgggcggcg aagaacgacg
     1441 gtcggcgcac actctcggcc gagttcgcgt tcaacgacga acggctacgc gcgcacgacg
     1501 cgtgactcgc gcgcgtcggt aacttccttg ctccccgaga cttcgagcag gagaagcacg
     1561 gagagaacga gctcgacgtc gtctccggcg accgtcacgg cgaattggcc gtcactcgat
     1621 gccaacgaaa ccgtctttcc catggcgaaa ccatcccttc acgaaccaag gttcggacgg
     1681 tgggttcgcc actcgctcgc taccgtcgag gcgaccagat tgcagacggt aggtcaacgc
     1741 tgcgcccgcc gccgtgtacc acggcggcac tcctggtatc ggcagccggc cggtcgaacc
     1801 tgaagtcgaa tgtcacgaag cgcgcgcacg gcgcggctcg gtatgggagc atcgcggcca
     1861 tgcgccccga cgacccggcg ctcgggccga gcgagatcct gatcggctgt gtgaacttcg
     1921 aagcggtgcc gcgcgacaag ggcgccacgc tcgagaagat gaacgccttc gttgccgaag
     1981 cggccgcacg tgactgcgac ctcgtcatct tccccgagtt ggccctcaac acgtggggca
     2041 agtgcgcggc atgcgccgag gcgcaccggc cgtgcgagtg gcaccgcggc caagccgaat
     2101 cggccgccgg cccgtcgtgt cgcgcgatcg tcgatatggc cgcggcgcat ggcgtgcatg
     2161 tcatctacgg ctacgaggag gcgggcgacg acgacgtcac cgcgatctac aactccgcga
     2221 acatcgttgc gccttccggg ctggtcggca cgtaccgcaa gctccacttg ggtatcccgc
     2281 tcgagaccga ccgcttcacg ccgggcgatg cgctgccggt gttcgagacc gcgctcggtc
     2341 cgatcggcat
//
LOCUS       DASUGA010000751         2127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1750985, whole genome shotgun sequence.
ACCESSION   DASUGA010000751 DASUGA010000000
VERSION     DASUGA010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750985"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1585
                     /locus_tag="VFX21_14800"
     CDS             <1..1585
                     /locus_tag="VFX21_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5097293.1"
                     /translation="AASILKPMLARGEPQTIGATTLDEYRKHLEKDAALERRFQPIKV
                     DEPTVPHTIEILKGLRERYEAHHRVTITDQAIVAAANLADRYISDRHLPDKAIDLIDE
                     AGSRLRIRRMQAPPDYRELEDEIAKVRKEKENAIEAQQFERAAKLRDREKELLEQRSA
                     KEQQWRAEGVDLFNEVNEESIAEVLALWTGIPVYKLTEEETAKLLRMEEELHKRIVGQ
                     HDAIKAVSQAIRRTRAGLKDPKRPSGSFIFLGPSGVGKTETAKTLAEFLFGDESAMIQ
                     LDMSEYMEKHTVSRLVGSPPGYVGYDEGGQLTEAVRRKPFSVVLFDEIEKAHPDVFNT
                     LLQILEDGRLTDAQGRSVDFKNTVIIMTSNLGTADLRKANVGFVKADEAVTYEKMKAK
                     VNEELKRHFRPEFLNRIDDVIVFHELTSDEITEIVDMLIKRVITQLETQGLGFELTHD
                     AKLLLAKKGYDPALGARPLRRAIQRMVEDPLSEKILWKEFRAGDKIVVDAADDEIVFR
                     TVEGVEPPPKELAGSGTPE"
     gene            1600..>2127
                     /locus_tag="VFX21_14805"
     CDS             1600..>2127
                     /locus_tag="VFX21_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097294.1"
                     /translation="MARVEQNGRALDTAILLDDVRDAGWTVGSWTRADDGGASIELTR
                     DFTGEAQLRARLAELGGASDVFGGPRVIHERSFLRARDELSVAADLRGLAANIAADEE
                     LAAALTAAGLDPASLEQQLTAELAGAFHLTVGVTLPNGRTEEVQLRSGDRQRVTAAHK
                     SFHANRVAAVAGVALL"
BASE COUNT          399 a          716 c          715 g          297 t
ORIGIN      
        1 cgccgcgagc atcctgaagc cgatgctcgc ccgcggtgag ccgcagacca tcggtgcgac
       61 gacgctcgac gagtaccgca agcacctcga gaaggacgcg gccctcgagc gccggttcca
      121 gccgatcaag gtcgacgagc cgacggtccc gcacacgatc gagatcctga agggtctgcg
      181 cgagcggtac gaggcgcacc accgcgtcac gatcaccgac caggcgatcg tcgccgccgc
      241 gaacctcgcc gaccgttaca tctccgaccg ccacctcccc gacaaggcga tcgacctcat
      301 cgacgaagcg ggttcgcgcc tgcgcatccg tcgcatgcag gccccgcccg actaccgcga
      361 gctcgaagac gagatcgcga aggtccgcaa ggagaaggaa aacgcgatcg aagcgcagca
      421 gttcgagcgg gccgccaagt tgcgcgaccg cgagaaagaa ctgctcgagc agcgctccgc
      481 caaggagcag cagtggcgcg ccgagggcgt cgacctcttc aacgaagtga acgaagagtc
      541 gatcgccgaa gtcctcgcgc tgtggacggg tatcccggtc tacaagctca ccgaggaaga
      601 gacggcgaag ttgctgcgca tggaggagga gctgcacaag cgcatcgtcg gtcagcacga
      661 cgcgatcaag gcggtctcgc aggcgatccg ccgtacccgc gccggcctga aggacccgaa
      721 gcgcccgagc ggttcgttca tcttcttggg cccgtcgggt gtcggaaaga ccgagaccgc
      781 gaagacgctc gcagagttct tgttcggtga cgagtcggcg atgatccagc tcgacatgtc
      841 cgagtacatg gagaagcaca cggtgtcgcg gctcgtcggt tcgcctccgg gctacgtcgg
      901 ttacgacgaa ggcgggcagc tcaccgaagc cgtgcggcgc aagccgttct cggtggtgct
      961 tttcgacgag atcgaaaagg cgcaccccga cgtcttcaac acgctcctgc agatcctcga
     1021 agacggtcgc ttgaccgacg cgcagggtcg ctccgtcgac ttcaagaaca cggtcatcat
     1081 catgacctcg aacttgggca cggccgatct gcgcaaggcg aacgtcgggt tcgtgaaggc
     1141 cgacgaggcc gtcacctacg agaagatgaa ggcgaaggtc aacgaggagc tgaagcgcca
     1201 cttccgcccc gagttcctga accgcatcga cgacgtcatc gtcttccacg agctgaccag
     1261 cgacgagatc accgagatcg tcgacatgct catcaagcgt gtgatcacgc agttggagac
     1321 acagggcctc ggcttcgagc tgacgcacga cgccaagctg ctgctcgcca agaagggcta
     1381 cgaccccgcc ctgggtgccc ggccgttgcg ccgtgcgatc cagcggatgg tcgaagaccc
     1441 gctgtccgag aagatcctct ggaaggagtt ccgggccggc gacaagatcg ttgtcgacgc
     1501 ggccgacgac gagatcgtgt tccgcacggt cgaaggcgtc gagcctccgc ccaaggagct
     1561 cgccggaagc ggtacgcccg aatagcactc gcggaggccg tggcgcgcgt cgagcagaac
     1621 ggccgggcgc tcgacaccgc catcctgctc gacgacgtcc gcgacgcggg ctggacggtg
     1681 gggagctgga cgcgggccga cgacggtggc gcgtcgatcg agctcactcg cgacttcacc
     1741 ggtgaggcgc agctccgcgc gcgactcgcg gagctgggcg gcgcgtcgga cgtcttcggc
     1801 ggtccacgcg tgatccacga gcggtcgttc ctgcgcgcgc gcgacgagct gtcggtggcc
     1861 gccgatctgc ggggtctggc cgcgaacatc gccgccgacg aggagctggc cgcggcgctg
     1921 accgcggccg ggctcgatcc cgcatcgctc gaacaacagc tcaccgccga gctggccggc
     1981 gcgtttcacc tgacggtcgg cgtgacgctg cccaacggtc gcaccgagga ggtccagctc
     2041 cggtccggcg accggcagcg ggtgaccgcg gcgcacaaga gcttccatgc gaaccgcgtc
     2101 gccgccgtcg ccggcgtcgc gctgctc
//
LOCUS       DASUGA010000752         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1333837, whole genome shotgun sequence.
ACCESSION   DASUGA010000752 DASUGA010000000
VERSION     DASUGA010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333837"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VFX21_14810"
     CDS             <1..410
                     /locus_tag="VFX21_14810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5097295.1"
                     /translation="IAGMGYLRSPKPVPAAKARNIVDEQANPPVAGPDLVVTTDKQGA
                     RVVLSISGELDAYTAPGLEDQIARLMTEGTSDIVLDLSQTEFLDSSGLRAILTAQRKL
                     TEQHGKLTLRAPSEPVTRLLEITGLTDNFATDG"
     gene            complement(432..788)
                     /locus_tag="VFX21_14815"
     CDS             complement(432..788)
                     /locus_tag="VFX21_14815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5097296.1"
                     /translation="MGLVQLRVRLVDSVSVAEVDGELDASNVDDVLSQLVAKMSNHAP
                     GLVLDLAPTQYLDSAGVRVLFELARLLRSRRQELRLAVPADGIVRKVLVLTAVADVVP
                     LHESVESAVAAMRPRD"
     gene            961..1347
                     /locus_tag="VFX21_14820"
     CDS             961..1347
                     /locus_tag="VFX21_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097297.1"
                     /translation="METKLTGETVRLTVPAALEYVRIVRLTASGVASRLGFDVEEIED
                     LRVAVDELTSLIVEAAVGNELEVVFAVYDDALHIDGEADAKPTGEPTVIEDLTGQILA
                     AVVDEWRLETTDGRLRFCCVKRLPAS"
     gene            1353..>2008
                     /locus_tag="VFX21_14825"
     CDS             1353..>2008
                     /locus_tag="VFX21_14825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5097298.1"
                     /translation="MALSSEERQRLKRKFVEYRQTHDRKLRNELIEAHRSLASHLARR
                     FANRGEPFDDLLQVAYLGMLKAVERFDPDRNLEFSTFATATVEGELKRHFRDKTWAVR
                     VPRRPQELHLRLGNAINELSQKLHRPPQISELAEELGVSEDSVLEAMEVGGAYRSTSL
                     DAKPTDGGESLTLESRLGTHDVGFDLAEHRVVLERLLEDLPERERTIVRLRFFEDMTQ
                     T"
BASE COUNT          369 a          696 c          642 g          301 t
ORIGIN      
        1 ggattgcggg catggggtac cttcgttccc cgaagccggt gccggcagcg aaggcgagga
       61 acatcgtgga cgaacaggcg aacccgcccg tcgctggccc tgacttggtc gtcacgaccg
      121 acaagcaggg tgcccgtgtc gtgctctcga tcagcggcga gctggacgcg tacaccgcgc
      181 ccggactcga agaccagatc gcgcgcctga tgaccgaggg cacttccgac atcgtgctcg
      241 atctgtcaca gaccgagttc ctcgattcgt cgggattgcg cgcgatcctc accgcgcagc
      301 gcaagctcac cgagcaacac gggaagctga cgttgcgggc gccgagcgag cccgtcacga
      361 ggctgctcga gatcacgggc ctcaccgaca acttcgcgac cgacggctga cgcgcgtaat
      421 tgcgcgcgta atcagtcgcg cgggcgcatc gccgcgacgg ccgactcgac gctctcgtgg
      481 agcggcacca catcggcaac ggcggtcaac acgagcacct tgcgcacgat gccgtccgcc
      541 ggcaccgcca accggagctc ctgacgccgg ctccgcaaca accgcgccaa ctcgaacagc
      601 acgcgcacac cggcgctgtc gaggtactgc gtgggcgcga gatcgaggac gagaccgggc
      661 gcgtggttcg acatcttcgc gaccaactgg ctcagcacgt cgtcgacgtt cgacgcgtcc
      721 agctcgccgt ccacttcggc aacggacacc gaatcgacga gacgtactcg tagctggacc
      781 agtcccatcg cgcgactcat agcaactgtt tgcccacgga gcgagtgtcg aatccccggc
      841 cgggtcaatt gtccggccga cggcgctcgt cgggcacgcc cggtttccac ccgatcgttc
      901 ccgggtaccg tttgtcatcc gcgggggtcg cgcccccgcg cccggaaatg gagggcgtac
      961 gtggaaacca agctcaccgg cgagacggtc cggctgaccg tccccgccgc gctcgagtac
     1021 gtgcgcatcg tgcggttgac cgcatccggc gtcgcctccc gcctcgggtt cgacgtcgaa
     1081 gagatcgaag acctgcgcgt cgcagtggac gagttgacca gcctgatcgt ggaggccgcg
     1141 gtcggcaacg agctcgaagt cgtgttcgcc gtctacgacg acgcgctgca catcgacggc
     1201 gaagccgacg ccaagccgac cggtgaaccg acggtcatcg aggacctcac cggccagatc
     1261 ctcgccgcag tcgtcgacga atggagattg gaaacaacgg atgggcgcct ccggttctgc
     1321 tgcgtgaagc ggttgccggc gagctgagaa cgatggctct ctccagcgaa gaacgacagc
     1381 ggctcaaacg gaaattcgtc gagtaccggc agacgcacga tcgcaagctc cgtaacgagc
     1441 tgatcgaggc gcaccggtcg ctcgcctcgc acctcgcgcg ccgtttcgcg aaccgcggtg
     1501 aaccgttcga cgatctcctg caagtcgcgt atctcggcat gctcaaagca gtcgagcgct
     1561 tcgaccccga ccgcaacctc gagttctcga cgttcgcgac cgcgaccgtc gaaggcgagc
     1621 tgaagcgcca cttccgcgac aagacgtggg ccgtgcgcgt gccgcgccgt ccgcaggagt
     1681 tgcacctgcg actcgggaac gcgatcaacg agctgtcgca gaagctgcac cgcccgccgc
     1741 agatctccga gttggccgag gagctcggcg tcagcgagga cagcgtgctc gaggcgatgg
     1801 aagtcggcgg cgcgtaccgc tcgacgtcac tcgacgccaa gcccactgac ggcggcgagt
     1861 cgctgaccct cgagagccgg ctcggcaccc acgatgtcgg cttcgacctc gccgagcatc
     1921 gcgtcgtctt ggaacgactg ctcgaagatc ttcccgaacg cgagcgcacg atcgtgcgcc
     1981 tccgcttctt cgaggacatg acccagac
//
LOCUS       DASUGA010000753         3005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_796457, whole genome shotgun sequence.
ACCESSION   DASUGA010000753 DASUGA010000000
VERSION     DASUGA010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796457"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VFX21_14830"
     CDS             complement(<1..942)
                     /locus_tag="VFX21_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018787407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX5097299.1"
                     /translation="MRHGLSLRARLFVAIVLVAVAVLAAASVATYVLVKRSIEENALQ
                     DMRERSSTLAELGTELQRAVPLTPEDNPRLSPQARQRRIAARDRARAALNLSGFNVVF
                     IGPDGELATGEALPNVADVVGLPPGLAADDLDTAALVAGDEVSGTKGDTVYLAQAIGE
                     SRAFTTVVVATDTIDTSVLTDAEPWLLAAAIALLGLAAGAAWLVARRLTRPIRDIERT
                     AQSLAAGDLSARAEVKPGTDPDLAALAATLNEMAAQLEAARGSERAFLLSVSHDLRTP
                     LTSIRGYAEALGDGTIDGSDPEARERAAAVITAEARRL"
     gene            complement(939..1625)
                     /locus_tag="VFX21_14835"
     CDS             complement(939..1625)
                     /locus_tag="VFX21_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5097300.1"
                     /translation="MQRHMERGTILVVDDEPNIADLVELYLRRDGYRVLKAGTGEDAL
                     RLAAAQSPRLVVLDVGLPDIDGLEVCRRLRATSAVPVIFLTARDTEVDRILGLEMGAD
                     DYVSKPFSPGELVARVKAVLRRADGVSGPPSDVVHAGQVEVDVARREVRVHGAPVPFT
                     TKEFDLIKFLAERSGLALSRQQILDGVWGYDWFGDVRTVDVHIAQVRKKVGHAVRIDT
                     VRGVGYRLDA"
     gene            1754..2332
                     /locus_tag="VFX21_14840"
     CDS             1754..2332
                     /locus_tag="VFX21_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097301.1"
                     /translation="MTKAQRADCRRAPARHSGATLRQELTLRMAAMARHPAVATSGVQ
                     ARVVHPADREQYARCRELGEVSDAYEGASVRMLATSDTGTVIGTARLTLWSPVVGFMV
                     EHEVDDPRRELVLGPRTAELSAMVAVGTTREQTHIWLTLTRLAYRVAGAHGLDHLYVI
                     ADPRHIIAIVSPGAYAISATAARIDLKARPVS"
     gene            complement(2329..>3005)
                     /locus_tag="VFX21_14845"
     CDS             complement(2329..>3005)
                     /locus_tag="VFX21_14845"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEX5097302.1"
                     /translation="YVMGTFPPGTRDFATYATVNEHFIAAHHAAVDVLRAGPGSFPVG
                     WTLSMSDWSIEGDNQEALDRTRMMHEGQFLEAARGDDYIGVQAYTRTRLSSEGLPLGP
                     EDGVPVVTAMGYEVWPQALAASVRYAAEKAQVPVYVTENGMGTDDDEQRIAYVHDSLD
                     GLLRCIDDGIDVRGYFHWSLLDNFEWAEGYAPRFGLVDVDRTTFARTPKPSAKWFGEI
                     ARRNAL"
BASE COUNT          436 a         1044 c         1079 g          446 t
ORIGIN      
        1 cagccgccgc gcctccgcgg tgatgaccgc ggccgcgcgc tcgcgcgcct cggggtcgct
       61 gccatcgatc gtgccgtcgc cgagtgcctc cgcgtagccg cgaatcgacg tcaacggcgt
      121 ccgcaggtcg tgcgacaccg acaacaggaa cgcccgctcc gacccgcgcg cggcttccag
      181 ctgcgcggcc atctcgttga gcgtcgccgc caaggccgcg aggtcgggat cggtaccggg
      241 cttcacttct gcacgcgcgg acaggtcgcc ggccgcgagc gattgcgcgg tccgttcgat
      301 gtcgcggatc gggcgcgtca gccgccgcgc gaccagccac gcggcaccgg ccgcgagccc
      361 gagcagcgca atggccgcgg cgagcaacca cggttcggcg tcggtgagca ccgatgtgtc
      421 gatcgtgtcg gtggcgacga cgacggtcgt gaacgcgcgg ctctcgccga tggcctgtgc
      481 gaggtagacg gtgtcgccct tggtcccgct gacttcgtcg ccggccacga gcgctgcggt
      541 gtcgaggtcg tccgccgcca gcccgggcgg caggcccacc acgtcggcga cgttcggcag
      601 cgcctcgccg gtcgccagct cgccgtcggg cccgatgaac acgacgttga accccgacag
      661 gttgagcgca gcgcgcgccc ggtcgcgggc ggcgatgcgg cgctggcgcg cctgcgggct
      721 gaggcgcgga ttgtcctcgg gggtgagggg gacggcccgc tgcagctcgg tgccgagctc
      781 cgccagcgtg ctcgaccgct cgcgcatgtc ctgcagcgcg ttctcttcga tcgagcgctt
      841 cacgagcacg taggtcgcga cgctggccgc ggcgagcacg gccactgcga ccaggacgat
      901 cgcgacgaag agccgcgcgc gcaacgacag gccgtgcctc acgcgtcgag gcggtagcca
      961 acgccgcgca cggtgtcgat gcgtaccgcg tgaccgacct tcttgcggac ctgtgcgatg
     1021 tgcacgtcga cggtgcggac gtcgccgaac cagtcgtagc cccagacgcc gtcgaggatc
     1081 tgctggcggc tgagcgcgag gccgctgcgt tcggcgagga acttgatgag gtcgaactcc
     1141 ttcgtcgtga acggcaccgg tgcaccgtgc acacgaacct cgcgccgagc gacgtcgacc
     1201 tcgacctgac ccgcgtgcac gacgtcggag ggcggtccgc tgacgccgtc ggcgcgccgc
     1261 agcactgctt tcacgcgcgc gacgagctcg ccgggggaga agggcttcga cacgtagtcg
     1321 tcggcgccca tctcgaggcc gaggatccgg tcgacctccg tgtcgcgcgc ggtgaggaag
     1381 atgacaggta ccgccgaggt cgcgcgcagg cggcggcaca cctcgaggcc gtcgatgtcg
     1441 ggcagcccga cgtcgagcac gaccagccgc gggctctggg ccgcggccag ccgcagcgcg
     1501 tcctcgccgg taccggcctt caggacccgg tagccgtccc ggcggaggta cagctcgacc
     1561 aggtcggcga tgttcggttc gtcgtcgacg acgaggatcg tcccgcgctc catatgacgc
     1621 tgcatcgtcc caccccgctt accgcctccg tgcgatgtcc atatgatggg ttcgttagcg
     1681 ggacgtgtga catgcgacgc aaatccaaga tcactgattg agggacatca ccacggtccg
     1741 tagtgtcggc agggtgacca aggcacaacg ggcggactgc cgacgggcac cggcgcggca
     1801 ctcgggggca acgctgcgcc aggagctgac cttgcgcatg gccgcgatgg cccgccaccc
     1861 ggcggtcgcg acgagcggcg tgcaggcccg ggtcgtgcac cccgccgacc gcgagcagta
     1921 cgcgcggtgc cgggagctgg gcgaggtgag cgacgcctac gaaggcgcat cggttcggat
     1981 gctcgcgacc tccgacacgg gcaccgtcat cggcactgca cggctcacac tgtggtcgcc
     2041 ggtggtcggc ttcatggtcg agcacgaggt cgacgacccg cgccgcgagt tggtgctcgg
     2101 gccgcgcacg gcggagctgt cggcgatggt ggccgtggga accacccgcg agcagacaca
     2161 catctggttg acgctgacgc gcctcgcgta ccgagtggcc ggcgcgcacg gcctcgatca
     2221 cctgtacgtg atcgccgacc cgcggcacat catcgcgatc gtgtcgccgg gcgcgtacgc
     2281 gatcagcgcg accgcggccc ggatcgacct gaaagcgcgt ccggtctctt agagcgcgtt
     2341 gcggcgcgca atctctccga accatttcgc gctgggcttc ggcgtgcgcg cgaaggtggt
     2401 gcggtcgacg tcgacgagcc cgaaccttgg cgcgtacccc tcggcccact cgaagttgtc
     2461 cagcagcgac cagtggaagt agccgcggac gtcgatgccg tcgtcgatgc agcggagcag
     2521 tccgtcgagc gagtcgtgca cgtatgcgat gcgttgttcg tcgtcgtcgg tccccatccc
     2581 gttctcggtg acgtagacgg ggacctgtgc cttctcggcc gcgtaccgga cggacgccgc
     2641 cagcgcctgc ggccacacct cgtaccccat ggcggtgacg acggggacgc cgtcctcggg
     2701 gccgagtggg agtccttcgg acgaaaggcg tgtgcgcgtg tacgcctgca caccgatgta
     2761 gtcgtcgccg cgcgccgctt cgaggaactg gccttcgtgc atcatgcgtg tgcggtcgag
     2821 cgcttcctgg ttgtcaccct cgatcgacca gtcgctcatc gacagcgtcc agccgacggg
     2881 gaacgaaccg gggcccgcgc gcaggacgtc gaccgcggcg tggtgcgcag caatgaagtg
     2941 ctcgttcacc gtcgcgtagg tcgcgaagtc gcgggtgccc ggcgggaagg tacccatgac
     3001 gtagc
//
LOCUS       DASUGA010000754         2275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1124029, whole genome shotgun sequence.
ACCESSION   DASUGA010000754 DASUGA010000000
VERSION     DASUGA010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124029"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1891)
                     /locus_tag="VFX21_14850"
     CDS             complement(<1..1891)
                     /locus_tag="VFX21_14850"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX5097303.1"
                     /translation="MKTEHCARGGCGGVIVDGYCDTCGLAPGPAAPAPASTETPTPRA
                     ATAATATLTPRAPARDGSKCSQPGCDGIIQDGYCNKCGLAGVVVAKRATVSLPAAPKP
                     KLAGSVAVPSVRSKRTRSGESRRVTARTGSTTTSHRIGAGLVDVPPVVLPDPKDVVLA
                     NPQVAPEKRFCASCGEAVGRDRAGREGRMEGFCPSCGHRFSFSPKLQPDDVVAGQYKV
                     VGCLAHGGLGWIYLARDLHVSERWVVLKGLLDAGDEDAMVAAVAERRFLASVEHASIV
                     KIFNFVQYEDAGYTVMEYVGGPTLKAVLKERRAKAGRTDPLPVEQAIAYMLEVLPAFA
                     YLHRIGLLYCDFKPDNVVLQGEVVKLIDLGGVRRADDDDSAVFGTAGYQAPEIAQLGP
                     SVASDLYTIARTLAVLILDLPGYQSTYKHSLPPVEEHDVLVQHESLTRFLQKATAERP
                     DDRFQSADEMAEQLLGVLREIVATETGSARPARSSLFTLYADSSTMTTGAAWQGASTW
                     LALPRLRVDPEDPAAAQLAALPDDGPKKTRELLDDIDPRTVEVELALAYTSLVLGESA
                     VAAQGMDRVEQQDPWDWRVDWIRGCMALASGELDDAWQRFDRVYSDVSGELAPKLALA
                     LTAELA"
     gene            complement(1888..>2275)
                     /locus_tag="VFX21_14855"
     CDS             complement(1888..>2275)
                     /locus_tag="VFX21_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097304.1"
                     /translation="APLDASCLDGERQGLRPWLARLRALAESGDWRAARRGLTEWDRV
                     ARGTLASAQEIVTVNQRPLRRRDELRGRLDVFNAKARGTGMSEDMTIAPLYTRARELL
                     WSAPVDLDEAERVVTQYGEAVGKAGR"
BASE COUNT          346 a          817 c          771 g          341 t
ORIGIN      
        1 acgcgagctc ggccgtcaac gcgagtgcga gcttcggcgc gagctcgccc gaaacatcgg
       61 agtacacccg gtcgaagcgc tgccacgcgt cgtcgagctc gcccgacgcg agcgccatgc
      121 acccgcggat ccagtcgacg cgccagtccc acgggtcttg ttgttcgacg cggtccatac
      181 cctgcgccgc gacggccgac tcgccgagca cgagcgacgt gtacgcgagc gcaagctcga
      241 cctccaccgt tcgcggatcg atgtcgtcca acagctcgcg cgtcttcttt ggtccgtcgt
      301 ccggcaaggc cgcgagttgc gcggcggccg gatcctcggg atcgacacgc agacgcggga
      361 gcgcgagcca cgtcgacgca ccctgccacg ccgcgcccgt cgtcatcgtc gacgaatcgg
      421 cgtacagcgt gaacagcgag ctgcgggccg gacgtgcgct cccggtctcg gtcgcgacga
      481 tctcgcgcaa cacgcccaac aactgctcgg ccatctcgtc ggcgctctgg aagcggtcgt
      541 cggggcgctc ggccgttgcc ttctgcaaga agcgcgtcag cgattcgtgc tgcacgagaa
      601 cgtcgtgctc ctcgaccggc ggcaacgagt gcttgtaggt cgactggtaa ccgggaaggt
      661 cgaggatcag cacggccagc gtgcgcgcga tcgtgtagag gtcggaggcc accgacggcc
      721 cgagttgcgc gatctccggc gcctggtatc ccgcggtgcc gaacaccgcg ctgtcgtcgt
      781 cgtcggcgcg ccgcacgccg ccgaggtcga tgagcttgac gacctcgccc tggagcacga
      841 cgttgtccgg tttgaagtcg cagtacagca ggccgatgcg gtgcaagtac gcgaacgcgg
      901 gcagcacctc gagcatgtac gcgatcgctt gttcgacggg cagcggatcg gtgcggcccg
      961 ccttcgcgcg ccgctccttc agcactgcct tcaacgtggg cccgccgacg tactccatca
     1021 cggtgtagcc ggcgtcctcg tactgcacga agttgaagat cttcacgatc gacgcgtgct
     1081 cgactgacgc gaggaaccgg cgttcggcaa ccgcggccac catcgcgtcc tcgtcacccg
     1141 cgtcgagcag gcccttgagc acgacccagc gttcggagac gtgcaggtcg cgggcgaggt
     1201 agatccagcc caagccaccg tgcgcgaggc agccgaccac cttgtactgg ccggccacga
     1261 catcgtcggg ttgcagcttc ggcgagaacg agaaccggtg cccgcacgag ggacagaagc
     1321 cctccatgcg accctcgcga ccggcacggt cgcgcccgac tgcttcaccg cacgacgcgc
     1381 agaaccgctt ctccggcgcg acctgcggat tggcgagcac gacgtctttg ggatcgggca
     1441 acacgacggg cgggacgtcg acgagcccgg caccgatgcg atgactcgtc gtcgtggagc
     1501 ccgtgcgggc ggtgacgcgc cggctttcac ccgaacgcgt gcgcttggaa cgcacactcg
     1561 gcaccgccac cgaaccggcc aacttcggtt tcggcgcggc cggaagcgac accgtcgcgc
     1621 gcttggcgac gacgacaccg gcgagaccgc acttgttgca gtagccgtct tggatgatcc
     1681 cgtcgcatcc gggctggctg cacttcgacc cgtcccgcgc cggcgctcgc ggtgtcagcg
     1741 ttgccgttgc ggcggtcgcg gcgcgtggag tgggcgtctc cgtgctcgcc ggcgccgggg
     1801 ccgccggacc gggcgcgaga ccacacgtgt cgcagtaccc gtcgacgatg acgccgccgc
     1861 atccaccacg cgcgcaatgt tcggtcttca tcgccctgcc ttcccgacgg cttcgccgta
     1921 ctgcgtgacg acgcgctcgg cttcgtcgag atcgaccggc gcggaccaca gcagctcacg
     1981 ggcgcgcgtg tagagcgggg cgatcgtcat gtcttcggac atcccggtac cccgcgcctt
     2041 cgcgttgaac acatcgaggc ggccgcgcaa ctcgtcgcgt cgccgcaggg gccgctggtt
     2101 cacggtcacg atctcctgcg cggacgcgag cgtgccgcgc gcgacgcggt cccattcggt
     2161 gaggccgcgc cgggccgccc gccagtcgcc actctcggcg agagcgcgca ggcgcgcgag
     2221 ccagggccgc agcccttggc gttcaccgtc gaggcacgac gcatcgaggg gcgcg
//
LOCUS       DASUGA010000755         4659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1613394, whole genome shotgun sequence.
ACCESSION   DASUGA010000755 DASUGA010000000
VERSION     DASUGA010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613394"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..360
                     /locus_tag="VFX21_14860"
     CDS             25..360
                     /locus_tag="VFX21_14860"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX5097305.1"
                     /translation="MGLSALAGDVEVAVLTATFDAKPGSEEALAAALARYVVLTRNEP
                     GCRNVDLVASATHGGRVLVIEKWDGADLVQGHLDSPLMTDMAGEVLPLLASKPDIDLY
                     DTISAHDLM"
     gene            complement(373..975)
                     /locus_tag="VFX21_14865"
     CDS             complement(373..975)
                     /locus_tag="VFX21_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097306.1"
                     /translation="MSKVTVTPDPFTLIAYDAAEIRAITEDIAAMIDFPTAVDIDIEV
                     DEELFAPLVGHASDFADGRAHVWVSGANFEDNRRPRTFSADQARRDLTLALLRAKDRT
                     TDEYADAPPDRELTRAERAAWDVHAAGRAAALGFEIRRQALLYEFRLQHGFSDVADAA
                     FVRLWDSPSVTFAGIREICVETGGAERGDSKIPVDLLRQK"
     gene            complement(1043..1885)
                     /locus_tag="VFX21_14870"
     CDS             complement(1043..1885)
                     /locus_tag="VFX21_14870"
                     /inference="COORDINATES: protein motif:HMM:NF016212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF455 family protein"
                     /protein_id="HEX5097307.1"
                     /translation="MKKFLGEHELARDDRFVRTKIEDRIFDPRGAKVAMRNTGPAKMA
                     IDRPAAGERSRSLMHGIFVGEIQALEGAGRTCYDFDLDDAPFQLKLDMARQCWDESRH
                     CEISVKLGDHMGTEIGDYTESTFLFAAACNPDPVLRLCGVNRALEGLAIDVFSSMKDF
                     GDESGDPVLEFCEDWMLADEVTHVKMGSDWLRRLTDNDPERRERALEFQRTVDSIFNL
                     GGFRGEDDENPIQLARKFRHLAGFTDEENDSLAALARQSTEQAREMAAMATAALDSSA
                     QGGS"
     gene            complement(1933..2583)
                     /locus_tag="VFX21_14875"
     CDS             complement(1933..2583)
                     /locus_tag="VFX21_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097308.1"
                     /translation="MTDHPKPRAAFAPWDRRELPGLFSVEETARRVGNYKWAEMKLFE
                     ALGGWVATVPELDVKLVLGRHTYHHAWHAELWDKRLPELREMNTSRLTVPPNPEFEKF
                     MDSVREPEAAEHTIEKLVGVYRVLIPRFIAVYTYHLNGTSTITDAPTIRSLKFILQDE
                     FEDWRDGEMLLQSLIDTPEKVQRAARRQEELEQLYLASGGIAGPGSLGEVSEAVGV"
     gene            complement(2583..3518)
                     /locus_tag="VFX21_14880"
     CDS             complement(2583..3518)
                     /locus_tag="VFX21_14880"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5097309.1"
                     /translation="MVGLTPQQQPRYLPEFALLAATTAYGSTFVLVQDTLDRVTPVGY
                     ILLRFAVGTIVLAPFAFARGWRRPSVPASTRSFVVASLAFGVVAFVGYWMQNLGIQRT
                     TTSQAAFITGLFVVFTPLIEMVVTKKAPPRNVVVAVVVSTIGLFLLTGADLTVHSGDL
                     FELGCAFTFGIWIYLGGVFSPRFDPITLTAVQLAIVALLAVPATMLGGLGHIDTQVVV
                     TVLVTGILCSAVGFAIQLWGQRFVEPSRAAVILLFEPVIAGFVGYAVGERLGVSGYVG
                     ALTILTGILIAESVTWRSAPSGQLTERSSTAENAP"
     gene            complement(3506..>4659)
                     /locus_tag="VFX21_14885"
     CDS             complement(3506..>4659)
                     /locus_tag="VFX21_14885"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX5097310.1"
                     /translation="MLATRAADASVPIVYANLVGGQDELVFDGASLVFDEQGHLVARA
                     RQFVEDLLVVDVDVRPAFRKRLLDPRGRASAASLPEVAVTEAHEHENTVTARVEQPLP
                     ELQEVYEALVLGTGDYVRKTGFEKVLVALSGGIDSAIVAAIAVDALGADNVVGVLMPS
                     RYSSGGSITDAEALAQNLGIRTHTVPIEPAHAAFEQMLGPVFEDSGWGVAGENVQARI
                     RGNVMMTISNRFGWMVLTTGNKSEMATGYATLYGDMAGGFAVIKDVEKTLVFALCRDL
                     NARAGREIIPQVIIDKPPSAELAPDQKDTDSLPPYDVLDPIIEGYVEQDLSIAELIAR
                     GHDRELVERVARMVDRNEYKRRQAPPGVRVSPKAFGKDRRLPITNRWSG"
BASE COUNT          772 a         1548 c         1596 g          743 t
ORIGIN      
        1 aaaaatgcct cttgacaagg cgaaatgggg ctctccgcac tggctggcga cgtcgaggtg
       61 gcagtgctga ccgcgacatt cgacgcaaag ccgggctccg aggaagcgct ggccgccgcg
      121 ctcgcgcgct acgtcgtgtt gacccggaac gaacccgggt gccgcaacgt cgacctggtc
      181 gcctccgcca cccacggcgg tcgggtgctg gtgatcgaga agtgggacgg cgccgacttg
      241 gtgcagggcc acctcgactc accactgatg acggatatgg cgggcgaggt cctgccgctg
      301 ctcgcgagca agcccgacat cgacctctac gacacgatct cagctcatga cttgatgtga
      361 gctgatctgc gcttacttct gacggagaag gtcgacgggg atcttggagt cgccccgctc
      421 ggcgccgccg gtctcgacgc agatctcgcg gatgccggcg aaggtcaccg acggcgagtc
      481 ccagagccgc acgaacgcgg catcggcgac gtcgctgaac ccatgctgga gccggaactc
      541 gtagagaagc gcctggcggc ggatctcgaa gccgagcgcc gcggcccgac cggccgcgtg
      601 gacgtcccag gcggcccgtt cggcccgcgt gagctcgcgg tcgggcggcg catcggcgta
      661 ttcgtcggtc gtgcggtcct tggcccgcag caacgcaagg gtcaggtcac ggcgcgcctg
      721 gtcggcggag aacgttcggg gccgccggtt gtcctcgaag ttggcgccgg agacccagac
      781 gtgcgcgcgc ccgtcggcga aatcagaggc gtggccgacg agcggggcga acagctcctc
      841 gtcgacttcg atgtcgatgt cgaccgccgt cgggaagtcg atcatcgccg cgatgtcttc
      901 ggtgatcgcg cggatctccg ccgcgtcgta ggcgatgagc gtgaacggat caggtgtgac
      961 cgtgaccttg ctcatggctc cctccgctac tgccgagggg cggcttcgcc gcaagaaggc
     1021 ttccgctgcg tccgcaccgt gctcacgagc cgccttgtgc agacgagtcg agcgcggccg
     1081 ttgccatggc cgccatctcg cgagcctgct cggtcgactg gcgcgccagc gcggcgagcg
     1141 agtcgttctc ttcgtcggtg aacccggcga gatgacggaa cttgcgcgcg agctggatcg
     1201 ggttctcgtc gtcttcgccg cggaagccac cgaggttgaa gatcgagtcg acggtgcgct
     1261 ggaactcgag cgcgcgctcg cgccgttcgg ggtcgttgtc ggtcaaacgt cgcaaccagt
     1321 cggagcccat cttcacgtgc gtgacttcgt cggcgagcat ccagtcttcg cagaactcga
     1381 gcaccgggtc accggactcg tcgccgaagt ccttcatcga gctgaacacg tcgatggcga
     1441 ggccttcgag cgcgcggttc acaccgcaca gccgcaacac cgggtcgggg ttgcacgcgg
     1501 ccgcgaagag gaacgtcgac tcggtgtagt caccgatctc ggtgcccatg tggtcgccga
     1561 gcttcaccga gatctcgcag tgacgcgact cgtcccagca ctggcgggcc atatcgagct
     1621 tgagttggaa cggtgcgtcg tcgaggtcga agtcgtagca cgtgcgaccg gcgccttcga
     1681 gcgcctggat ctcaccgacg aagatgccgt gcatcagcga acgggagcgc tcccccgcgg
     1741 ccggccggtc gatcgccatc ttggccgggc cggtgttgcg catcgcgacc ttcgcaccgc
     1801 gcggatcgaa gatgcggtct tcgatcttgg tgcggacgaa acgatcgtcg cgggccagct
     1861 cgtgctcacc aaggaacttc ttcatgaaat ggctccctcg ctgcgccgtc cgtggctctg
     1921 ctgcgtccgc actcagactc cgaccgcttc ggacacctcg cccaggctac ccgggccggc
     1981 gatgcccccg gacgccaggt agagctgctc cagctcctcc tgccggcggg ccgcacgctg
     2041 gaccttctcg ggcgtgtcga tcaacgattg gagcagcatc tcgccgtcgc gccagtcctc
     2101 gaactcgtct tgcaggatga acttcaacga ccggatcgtc ggcgcgtccg tgatcgtgct
     2161 cgtgccgttc aagtggtacg tgtagaccgc gatgaagcgc gggatgagca cccggtagac
     2221 gcccacgagc ttctcgatcg tgtgctccgc ggcttcgggc tcgcgcaccg aatccatgaa
     2281 cttctcgaac tcggggttcg gcggcaccgt gaggcgcgac gtgttcatct cacgcagttc
     2341 gggcaaacgc ttgtcccaca gctcggcatg ccacgcgtgg tgatacgtgt gccggccgag
     2401 cacgagcttc acgtcgagct ccggcacggt cgcgacccat ccgccgagcg cctcgaacaa
     2461 cttcatctcg gcccacttgt aattgccgac gcgacgcgcg gtctcttcga cagagaacag
     2521 gcccgggagc tcacggcggt cccagggcgc gaaggcagca cgaggtttgg ggtgatccgt
     2581 catcagggcg cattctcggc ggtacttgac cgctcggtca actggccgga cggtgcggaa
     2641 cgccaggtga cggactcggc gatcaggatc ccggtgagga tcgtgagcgc accgacgtag
     2701 ccgctgacgc cgagccgctc gccgaccgcg tagccgacga agccggcgat caccggttcg
     2761 aacaacagga tgaccgcggc ccggctcggt tcgacgaacc gctggcccca gagctggatc
     2821 gcaaagccga ccgcgctgca gaggatgccg gtgacgagca cggtgacgac cacttgcgtg
     2881 tcgatgtggc cgaggccgcc gagcatcgtc gcgggaaccg ccagcagcgc gacgatcgcg
     2941 agctgcacgg cggtgagcgt gatcggatcg aagcgcggtg agaacacgcc accgaggtag
     3001 atccagatgc cgaacgtgaa cgcgcacccg agctcgaaga gatcgccgct gtgcaccgtg
     3061 aggtcggcgc cggtgagcaa gaacaacccg atcgtcgaca cgacgaccgc gacgacgacg
     3121 ttgcgcggcg gtgccttctt cgtgacgacc atctcgatca acggcgtgaa cacgacgaac
     3181 agaccggtga tgaacgccgc ctgcgaggtc gtcgtgcgtt ggatgccgag gttctgcatc
     3241 cagtagccga cgaacgcgac gacaccgaag gcgagggacg cgacgacgaa cgaacgcgtc
     3301 gacgcgggca cgctgggccg ccgccagccg cgcgcgaacg caaacggcgc gagcacgata
     3361 gtgccgacgg cgaagcgcag caggatgtac ccgaccggcg tgacgcgatc gagcgtgtcc
     3421 tgcacgagga cgaacgtcga cccgtacgcc gtcgtcgccg cgagcaacgc gaactcgggc
     3481 agatagcgcg gctgttgctg tggagtcagc ccgaccaacg gttcgtgatc ggcagtcgcc
     3541 ggtccttgcc gaacgccttg ggcgacaccc gcacgcccgg cggcgcctgg cggcgcttgt
     3601 actcgttgcg gtcgaccatg cgcgcgacgc gctcgaccag ctcacggtcg tggccgcggg
     3661 cgatcagctc cgcgatcgac aggtcttgct cgacgtagcc ctcgatgatc gggtcgagaa
     3721 cgtcgtacgg cggcagcgag tcggtgtcct tctggtcggg cgccaactcc gccgacggcg
     3781 gcttgtcgat gatcacctgc gggatgatct ctcgacccgc gcgcgcgttg agatcgcggc
     3841 acagcgcgaa gacgagcgtc ttctcgacgt ccttgatgac ggcgaagccg ccggccatgt
     3901 cgccgtagag cgtggcgtac ccggtcgcca tctcgctctt gttcccggtg gtcagcacca
     3961 tccacccgaa gcggttggag atcgtcatca tcacgttgcc acggatgcgg gcctgcacgt
     4021 tctcgcccgc gacaccccaa ccgctgtctt cgaacacggg gccgagcatc tgctcgaagg
     4081 cggcgtgcgc gggttcgatc gggacggtgt gcgtacggat gccgaggttc tgcgcgagtg
     4141 cttcggcgtc ggtgatgctg ccgccgctcg aatagcgcga cggcatgagg acgccgacga
     4201 cgttgtcggc accgagcgcg tcgaccgcga tcgcggcgac gatcgcggag tcgatcccgc
     4261 ccgagagcgc gacgagcacc ttctcgaacc cggtcttgcg cacgtagtcg cccgtgccga
     4321 gcaccagtgc ctcgtagacc tcctgcagct cgggcaacgg ctgttcgacg cgcgcggtga
     4381 cggtgttctc gtgctcgtgc gcctcggtga cggcgacctc cggcagcgac gccgcgctgg
     4441 cgcgaccgcg ggggtcgagc agccgcttgc ggaacgcggg gcggacgtcg acgtcgacga
     4501 cgagcaggtc ttcgacgaac tggcgggcgc gggcgacgag gtgcccttgc tcgtcgaaca
     4561 cgagcgacgc gccgtcgaac accaactcgt cctggccgcc gacgagattc gcatagacga
     4621 tcgggaccga cgcgtcggcg gctcgcgtcg cgagcatcg
//
LOCUS       DASUGA010000756         3811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1751449, whole genome shotgun sequence.
ACCESSION   DASUGA010000756 DASUGA010000000
VERSION     DASUGA010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1751449"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2620)
                     /locus_tag="VFX21_14890"
     CDS             complement(<1..2620)
                     /locus_tag="VFX21_14890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5097311.1"
                     /translation="MDTMEPGSISSSIADETGAAALDPDPLDPVLIDPEADDDEPGQR
                     LPPVVVVVVTKDPGPWLEATLRSLAAQDYPQLAVLVLDNGSADDPTARIAQALPTAFV
                     RRRDADDGFAAAANDALAAVEGATFLLFCHDDVTLDAGAVSVMVEEAYRSNAGIVGPK
                     VVDSEHPEILLEVGMTIDHYAVPFSGIEPDEVDQEQHDAVRDVFFVSHTVMLVRDDLF
                     RELDGFDAATDPGADDVDLCWRARLAGARVVVAPGARVLHQRAPVVEARAPRAKPAVE
                     VRAETTARIRVLCKSYSSLALIWVLPTAFVLGLAEAAGLVLSGRFAAARAVVAGWLAA
                     FRHPGELRRARKATQRLRRVEDGDVRDLMIRGSARVRTYFTQRVHAGDRLAEVSNRTR
                     ERMTETGVRMRRAPAIIAAILGVVVFFSVRSLLFGSVPQVGSFLAWPAVGDTWSTFTG
                     SWRTTLMGAPDAATPAFGLMTIINGLLLGHEGWARSLIVGGALPLGAFGAYRLTRPFA
                     QSALPGVAAAVAYAANPIARNAIFGGELGPLVLFALAPFVFHAFVRAVDAENPTTRSR
                     AHVLGTITILSAVAGAVWPPAIAVGLAFAIAFLVTAPFAGTTPGALRGAAYGALATVA
                     AAILLAPWSFTLVGADAATWGAQPRPALSLGEVLHFHTGSMGAGFASWGIVFAAVVPL
                     LIATGPRFVWAARAWVLAVLSYACVWLPGRIDAGAPALAPDGLLVLAGIALAFAAGLG
                     VAAVLDDLRRFHFGWRQVTTIVGVAGLTVAFFGLAADSLSGRFGLDGQDWPTTYAWMG
                     DTPAVGGFRTLWVGDPVILPLDPKTAGAVGFATTRDGSGDVRALWAAPESDADRTLAS
                     ALDAAQSGATARLGHLL"
     gene            2759..3211
                     /locus_tag="VFX21_14895"
     CDS             2759..3211
                     /locus_tag="VFX21_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097312.1"
                     /translation="MANYTKTLTDAGYIVVGLGVMGFQQAQTRGRELSERLSQASECI
                     SNRAKQAQAKLDEQSKTAGTQVKAAQAKAATQVQAAKAQADTAKAQATEQFRVTVEQA
                     KALRDQLNKRTQPIVDQAKATVGQVPERVVSAIEPVTARVREMTGSAA"
     gene            3247..>3811
                     /locus_tag="VFX21_14900"
     CDS             3247..>3811
                     /locus_tag="VFX21_14900"
                     /inference="COORDINATES: protein motif:HMM:NF014042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HEX5097313.1"
                     /translation="MLATLAGGVGAARFLAGLQRIVDPSDVVAIVNTADDDEFHGLHV
                     SPDLDSVTYTLAGASNVAQGWGLEGETFATLDALTRYDVPTWFRLGDKDLATHLFRTQ
                     RLRAGAPMSTVTAEIAAAWGVNCRLLPMTDDRVRTRITVQRTGGTEELAMQAWFVGER
                     AEPPVVAVRFEGAEAATPAPGVLDALET"
BASE COUNT          654 a         1329 c         1349 g          479 t
ORIGIN      
        1 cgaggagatg gccgaggcga gcggtcgcgc ccgactgggc cgcgtcgagc gccgacgcga
       61 gtgtgcgatc agcatccgat tcgggtgcag cccacagcgc gcgcacgtcg ccgctgccgt
      121 cgcgcgtcgt tgcgaacccg acggcgccgg cggtcttggg atcgagcggc aggatcaccg
      181 gatcgcccac ccacaacgtg cggaagcccc cgactgcggg ggtgtcgccc atccaggcgt
      241 atgtggtcgg ccagtcttgc ccgtcgagcc cgaaccggcc cgacagcgaa tcagccgcga
      301 ggccgaagaa cgcgaccgtg agcccggcga cacccacgat cgtggtcacc tgacgccagc
      361 cgaagtggaa ccggcgaagg tcgtcgagca ccgcggccac acccaggccg gccgcgaacg
      421 cgagcgcgat cccggccagg acgagcaggc cgtcgggcgc cagtgccggc gcgccggcgt
      481 cgatgcggcc cggcagccac acacacgcgt acgacagcac cgcgagcacc cacgcgcgtg
      541 ccgcccacac gaagcgcgga ccggtcgcga tcaacagcgg cacgaccgcg gcgaacacga
      601 ttccccacga cgcaaacccg gcacccatcg aaccggtgtg gaagtggagc acctcgccga
      661 gcgacaacgc cggccgcggc tgcgcacccc aggtcgcggc atcggcgccg accaacgtga
      721 acgaccacgg cgccagcaag atcgcggcgg cgacggtcgc gagcgcgccg tacgccgcgc
      781 cccggagcgc gccgggcgtg gtgcccgcga acggcgccgt gacaaggaat gcgatcgcga
      841 acgcgagccc gaccgcgatc gcgggtggcc acaccgcgcc ggcgaccgca ctgaggatcg
      901 tgatcgtgcc aagaacgtgc gcgcggctgc gcgtcgtggg gttctcggcg tcgacggctc
      961 gcacgaacgc gtggaagacg aagggtgcga gggcgaaaag gacgagcgga ccgagttcac
     1021 cgccgaagat cgcgttgcgc gcaatcgggt tggctgcgta cgcaacggcg gcagcaacgc
     1081 cgggcagcgc cgactgcgca aagggccgcg tcaagcggta cgcgccgaac gcgccgagcg
     1141 gcagtgcgcc gccgacgatc aacgagcgcg cccagccctc gtggccgagc aacaggccgt
     1201 tgatgatcgt catcaggccg aatgccgggg tggcggcgtc gggcgcaccc atgagggtcg
     1261 tgcgccagga cccggtgaac gtcgaccacg tgtcgccgac ggcgggccag gcgaggaagc
     1321 tgccgacctg cggcaccgac ccgaacagca gcgaacgcac gctgaagaac acgacgacac
     1381 cgagaatcgc ggcgatgatc gcgggggcgc ggcgcatccg gacgccggtc tcggtcatgc
     1441 gttcgcgcgt gcggttcgac acctcggcga ggcgatcgcc cgcgtgcact cgctgcgtga
     1501 agtacgtccg cacgcgcgca ctgccgcgga tcatgaggtc gcgcacgtcg ccgtcttcga
     1561 cgcggcgcag gcgttgcgtc gccttgcgtg cgcgtcgcaa ctcgcccgga tgccggaacg
     1621 cggccaacca acctgcgacg accgctcgcg cggccgcgaa gcgacccgag agcacgagcc
     1681 cggccgcttc cgcgagcccg aggacgaacg ccgtcgggag cacccagatc agcgcgagcg
     1741 acgaatacga cttgcacagc acgcggatcc gagcagtcgt ctcggcgcga acctcgactg
     1801 cgggcttggc gcgcggcgct cgcgcttcga cgaccggcgc gcgttggtgg agcactcgcg
     1861 caccgggcgc gacgacaacg cgtgcgccgg ccagacgcgc gcgccagcag aggtcgacgt
     1921 cgtcggctcc ggggtcggtg gcggcgtcga acccgtcgag ctcgcgaaag aggtcgtcgc
     1981 gcacgagcat gacggtgtgc gacacgaaga agacgtcgcg cacggcgtcg tgctgttcct
     2041 ggtcgacctc gtcgggttcg atgccggaga agggaacggc gtagtggtcg atcgtcatgc
     2101 cgacctcgag caggatttcc gggtgctcgg agtcgacgac cttgggcccg acgatcccgg
     2161 cgttggaccg gtaggcctct tcgaccatga cactcaccgc gccggcgtcg agggtgacgt
     2221 cgtcgtggca gaacaagagg aacgtggcgc cctccaccgc ggcgagtgcg tcgttggccg
     2281 cggcggcgaa accgtcgtcg gcgtcccgcc gacgcacgaa tgcggtcggg agcgcctgcg
     2341 cgatgcgcgc ggtcggatcg tccgcggagc cgttgtcgag cacaagcacc gcgagctgtg
     2401 ggtagtcctg cgcagcgagc gaccgcaggg ttgcttccag ccagggcccg gggtccttgg
     2461 tgaccacaac gacgacgacc ggaggcaggc gttggccagg ctcgtcgtcg tcagcctcgg
     2521 ggtcgatgag caccggatcc aacgggtccg ggtccagggc cgctgcgccc gtttcgtccg
     2581 caatggacga gctgatcgat cccggttcca tcgtgtccat gaggccgggg gaaccctagc
     2641 ccggccttct tcggaacttt gtgcgggaga tcacgagtgc ttgctggagt tagcacctcg
     2701 tgctatggtc gcgagcacat tggcaacgcc actccacccc tccaacaagg attgaccgat
     2761 ggccaactac accaagaccc tcaccgacgc gggctacatc gtcgtcggcc tcggcgtcat
     2821 gggcttccaa caggcgcaaa cccggggacg cgagctctcg gagcgtttgt cccaggcgag
     2881 cgaatgcatc tccaaccgcg ccaagcaagc gcaggcgaag ctcgacgagc agtcgaagac
     2941 tgccggcact caggtcaagg ccgcgcaggc caaagccgcc acccaggtgc aggccgccaa
     3001 ggcgcaggcc gacaccgcca aggcgcaagc gaccgagcag ttccgtgtca ccgtcgagca
     3061 agcaaaggcg ctgcgcgacc agttgaacaa gcggacgcag ccgatcgtcg accaggcgaa
     3121 ggcaaccgtc ggccaagttc ccgagcgagt cgtttcggcg atcgaaccgg ttactgcgcg
     3181 ggtgcgcgag atgaccggca gcgccgcctg agttgtttcc agtagcccgg cgatagggtc
     3241 gcgagcatgc tcgcgaccct tgccggtggt gtcggtgccg cccgctttct cgccgggctc
     3301 cagcgcatcg tcgatccgtc cgacgtcgtc gcgatcgtga ataccgccga cgacgacgag
     3361 ttccacgggt tgcacgtctc ccccgacctc gactcggtca cctacacgct ggccggcgcg
     3421 tcgaacgtcg cccaaggttg gggtctcgag ggtgagacgt tcgcgaccct cgacgcgctg
     3481 acgcgttacg acgtgccgac gtggttccgc cttggcgaca aggacctcgc cacgcatctc
     3541 tttcgcacgc agcgcttgcg cgccggcgcg ccgatgtcga ccgtcaccgc ggagatcgcg
     3601 gccgcgtggg gcgtgaactg ccggctgctg ccgatgaccg acgaccgcgt gcggacacgc
     3661 atcaccgtgc aacgaacggg cggcaccgag gagctggcca tgcaggcctg gttcgtcggt
     3721 gagcgagccg agccgccggt ggtcgccgtg cgcttcgagg gcgccgaagc cgccacaccg
     3781 gcgccgggcg tcctcgacgc gctggagacc g
//
LOCUS       DASUGA010000757         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_502100, whole genome shotgun sequence.
ACCESSION   DASUGA010000757 DASUGA010000000
VERSION     DASUGA010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502100"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /gene="era"
                     /locus_tag="VFX21_14905"
     CDS             complement(<1..870)
                     /gene="era"
                     /locus_tag="VFX21_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020105608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX5097314.1"
                     /translation="MSDQPEFLSGFAAIVGRPNVGKSTIVNQLVGRKVSIVSDRPQTT
                     RTQIRGVRTTATSQIVLLDTPGVHKPRTLLGERANSRALTTLAEVDVVCFVVEADARI
                     GPGDRFVAENIASVSTPVICVVNKVDLAKPDEIAQRLASAAELGDYAAFVPVSARTGD
                     GLDALVGEIEARLEPGPHYYPDGMITDQPESFLAAELVREKLLAVARDELPHSIAVTT
                     EEVDERETADGPLLALRVVIRVERESQKGIVIGKGGSVIKQAGTDARRELEALLGTRV
                     HLETHVKVEPDWQR"
     gene            complement(863..>2124)
                     /locus_tag="VFX21_14910"
     CDS             complement(863..>2124)
                     /locus_tag="VFX21_14910"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX5097315.1"
                     /translation="IVILFFFSILLAAAETAFLRMNRIKALALEEEGDKRATKLVRML
                     ERPERTVNAVTLLALAAQLITSYLLGILFGGAGVWLVVLSLVLNVVFFFVFAEAAPKT
                     WAVQHTDRAALRTSGFLWFLTGFPPVRFLVRSLLGFVNLILPGKGLKQGPFVTEEEIR
                     QMADVAAQEAAIETEERELIHSIFEFGDTVVREVMLPRPDMVAVEADATVDEGIALAI
                     GAGKSRLPAFDDSTDNIVGLVFLKDLVARTAADQGTELVRNSLRPAHFVPESKRVAEL
                     LREMQTEKFHMAIVVDEYGDTAGLVTMEDLLEEIVGEITDEYDVEEPQIEQLGPDRLR
                     VPGKTLIGEVNELLDTELPDEEWDTVAGLVFNSLGHVPVEGECTTFDGIEFCAERVEG
                     KRIVTVLIRRLEPHELPSEENASVQGE"
BASE COUNT          336 a          736 c          704 g          348 t
ORIGIN      
        1 ccgctgccag tccggttcga ccttcacgtg cgtctcgaga tggacccggg tgccgagcag
       61 cgcctccaac tcccgccgcg cgtcggtgcc ggcctgtttg atgacactgc cgcccttccc
      121 gatgacgatg cccttctgcg actcccgctc gacccggatg accacgcgga gcgcgagcaa
      181 cggcccgtcc gcggtctcgc gctcgtcgac ctcttcggtc gtgaccgcga tcgagtgcgg
      241 cagctcgtcg cgcgcgacgg ccagcagctt ctcgcgcacc agctccgcgg cgaggaacga
      301 ctcgggctga tcggtgatca tgccgtccgg gtagtagtgc ggccccggct cgaggcgagc
      361 ttcgatctcg ccgacgagcg cgtcgaggcc gtcgccggtg cgggccgaca ccggcacgaa
      421 cgcggcgtag tcgccgagct cggccgccga cgccagccgc tgcgcgatct cgtccggctt
      481 ggccaggtcg accttgttga cgacgcagat caccggcgtc gacaccgacg cgatgttctc
      541 ggcgacgaag cggtcgcccg ggccgatgcg cgcgtcggcc tcgaccacga agcagacgac
      601 gtcgacttcc gcgagcgtcg tcaatgcgcg cgagttcgcg cgttcgccga gcaacgtgcg
      661 cggcttgtgc acgcccggcg tgtcgagcaa gacgatctga ctggtcgccg tcgtgcgcac
      721 accacggatc tgcgtgcgcg tcgtctgcgg gcgatcggag acgatggaga ccttgcggcc
      781 gaccaactgg ttgacgatcg tggacttacc gacgttcggc cgtccgacga tcgccgcgaa
      841 ccccgacagg aactccggtt ggtcactcac cctgcacgga ggcgttttct tcggacggca
      901 gctcgtgggg ttcgaggcgg cggatcagca cggtcacgat ccgcttgccc tcgacccgtt
      961 ccgcgcagaa ctcgatgccg tcgaacgtcg tgcactcgcc ctcgaccggc acgtggccga
     1021 gcgagttgaa cacgaggccg gcgacggtgt cccactcctc gtcgggcagt tcggtgtcga
     1081 gcagctcgtt cacctcgccg atcaaggtct tgcccggtac gcgcaagcgg tcggggccga
     1141 gctgttcgat ctgcggctcc tcgacgtcgt actcgtcggt gatctcgccg acgatctctt
     1201 cgagcaggtc ttccatggtc acgaggccgg cagtgtcgcc gtactcgtcg acgacgatcg
     1261 ccatgtggaa cttctcggtc tgcatctcgc ggagcagttc cgcgacgcgt ttggactcgg
     1321 gcacgaagtg cgcgggccgc agactgttgc ggaccagctc ggtgccttgg tcggcggcgg
     1381 tgcgcgcgac gaggtccttc aggaacacga ggccgacgat gttgtcggtc gagtcgtcga
     1441 acgcgggcag gcgcgacttg ccggcgccga tcgcgagcgc gatgccctcg tcgaccgttg
     1501 cgtccgcttc gaccgcgacc atgtcggggc gcggcagcat cacctcacgc acgaccgtgt
     1561 cgccgaactc gaagatcgag tggatcaact cgcgttcctc ggtctcgatc gccgcctctt
     1621 gcgcagcgac gtcggccatc tggcggatct cttcttcggt gacgaacggc ccctgcttca
     1681 ggcccttgcc cggcaagatc aagttcacga acccgagcag gctgcgcacg aggaagcgca
     1741 ccggcgggaa gccggtgagg aaccacaaga aacccgacgt ccgcagtgcg gcccgatcgg
     1801 tgtgctgcac cgcccacgtc ttcggcgcgg cctccgcgaa cacgaagaag aagaccacgt
     1861 tgagcacgag cgagagcacg acgagccaca cgcccgcgcc gccgaacagg atgccgagca
     1921 ggtacgacgt gatcaactgc gcggccagcg ccagcaacgt caccgcgttg accgtgcgct
     1981 ccgggcgttc gagcatccgc acgagcttcg tcgcccgctt gtcgccctct tcctcgagcg
     2041 cgagcgcctt gatgcggttc atccgcaaga acgcggtctc ggccgccgcg aggaggatcg
     2101 agaagaagaa caggatgacg atga
//
LOCUS       DASUGA010000758         2837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1613920, whole genome shotgun sequence.
ACCESSION   DASUGA010000758 DASUGA010000000
VERSION     DASUGA010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613920"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..954
                     /locus_tag="VFX21_14915"
     CDS             34..954
                     /locus_tag="VFX21_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097316.1"
                     /translation="MNKHGVIAIWCARLVWALLPVTIGDALGDALDGWSTGSARTAAV
                     LCWAAWAAGEVALLAPRPWGLTYLRITAPAVTAIAIVAAFSAGAAPAALAVVGAIAAT
                     TLALSARVAQATGNALAYGDETRYPLRIPTPLLVTLVPLAIVVALASVAAGPLLLADE
                     RVPAGAVACVIGVPAVVLAVRSLHSLSKRWVVLVPAGLVVVDPLALTEPTLVRREQIA
                     SLSRAPRRQIATTTLDLTLGTTFTPTLLETREPVNFTRRRGRAGAEMVATMSVLVVPV
                     LVRELLATAAARRIPVQPAAIPPPSTMSPS"
     gene            complement(909..1931)
                     /gene="mnmA"
                     /locus_tag="VFX21_14920"
     CDS             complement(909..1931)
                     /gene="mnmA"
                     /locus_tag="VFX21_14920"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX5097317.1"
                     /translation="MKVMAMMSGGVDSSVAAALLLRQGHEVTGVTLKLWGGVSDSGCC
                     SVADVEDARRVAAQLDIPHSVFNFTDAFDEHVVAPYTAAYEAGRTPNPCVECNRTMKF
                     GRLLARAEQLGFDAVATGHHARVVTTPDGPMLARGEDAAKDQSYVLYMLGRRELERTL
                     LPVGEMSKPDVRAKAAALGLRTASKKESMDVCFITRGGREAFLGARIERRPGVVVDTS
                     GNVVAQHDGVDRFTIGQRRGLAVAAGARRYVVDVDAASAVVTIGERDDLLRPSITLTD
                     ICWSTGRAPAGPVLAQWRAHGAAVPAVVDGDVVTFDAPQARVAPGQVVVCYDGDIVLG
                     GGIAAG"
     gene            complement(1942..>2837)
                     /locus_tag="VFX21_14925"
     CDS             complement(1942..>2837)
                     /locus_tag="VFX21_14925"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX5097318.1"
                     /translation="ERSGLDGVVTTGIEHKAVLGACARLGRDGFRVTTVGAGLDGAVD
                     LDALAGALDDRTAVVSVMTVNNETGVQQPLDAVAALVREHAPHAVLHTDAVQAPQWRD
                     LRIATSGAQLVAISGHKFGGPKGVGALVVRDDVALVPLVEGGGHERDLRAGTQNVAGI
                     VAFATALRITHEQRAAETARIAALRDRFEAGLLAAIDGIVVNGDPARRVEGLLHVAIP
                     DVEAETLLVALDQAGVFAASGSACSSGAVDPSHVLIAMGIPRERALRSVRFSLGYAST
                     DDEIERALTVVPEVVAKLRAA"
BASE COUNT          394 a         1033 c          999 g          411 t
ORIGIN      
        1 gaactcacgg cgtgacgtca cgcccaactc ggaatgaaca agcacggtgt gatcgcgatc
       61 tggtgcgcgc gcctcgtgtg ggcactgctg ccggtcacca tcggggacgc gctcggcgac
      121 gcgctcgacg gctggtcaac gggctcggca cggacggcag cagtgttgtg ttgggcggcg
      181 tgggccgccg gtgaagtcgc gttgctcgca ccgcgaccct ggggactcac ctacctccgc
      241 atcaccgcgc cggcggtcac ggcgatcgcg atcgttgctg cgttctcggc cggtgccgcg
      301 ccggccgcgc tcgcagtcgt cggggccatt gccgccacca cactcgcgct gtccgcacgc
      361 gtcgcacagg cgaccggcaa cgcactcgcg tacggcgacg agacgcgcta cccgttgcgc
      421 atcccgacgc cgctgctcgt cacgctggtg ccgctcgcga tcgtcgtcgc gttggcgagc
      481 gtcgcggccg gtccgttgtt gttggcggac gaacgcgtgc cggccggtgc ggtcgcgtgt
      541 gtgatcggcg tgcccgccgt cgtgctcgcg gtgcgatcgt tgcactcact gtcgaaacgg
      601 tgggtcgtgc tcgtgcccgc cgggctcgtc gtcgtcgacc cgctcgcact gaccgaaccg
      661 acactcgtgc gacgcgagca gatcgcgtcg ttgtcgcgtg cgccgcgccg tcagatcgcg
      721 acgacgaccc tcgatctcac gctcggcacg acgttcacgc ccacgctcct ggagacgcga
      781 gagcccgtca atttcacgcg gcggcgcggg cgcgcgggcg ccgagatggt tgcgaccatg
      841 tcggtgctcg tcgtccccgt gctggtccgc gagctgctgg cgacggcagc cgcgcggcgc
      901 atcccggttc agccggccgc gatacccccg ccgagcacga tgtcgccgtc gtagcagacg
      961 accacctgcc cgggcgcgac gcgtgcttgt ggcgcgtcga aggtgacgac gtcaccgtcg
     1021 acaacggcgg gcacggccgc accgtgggcg cgccattgcg cgagcaccgg accggcgggc
     1081 gcgcgaccgg tcgaccagca gatgtcggtg agcgtgatcg acggccgcag caggtcgtct
     1141 cgttcgccga tcgtgacgac cgcgctcgcg gcgtcgacgt cgacgacgta gcggcgcgcg
     1201 cccgcggcga cggccaggcc gcgccgctgc cctatcgtga atcggtcgac accgtcgtgc
     1261 tgcgcgacga cgttgccact cgtgtcgacg acgacaccgg gtcgccgttc gatacgggcg
     1321 cccaagaagg cctcgcgtcc accacgcgtg atgaagcaga cgtccatgct ctccttcttc
     1381 gatgccgtgc gcaggcccag tgcggccgcc ttggcgcgga cgtcgggctt cgacatctcg
     1441 ccgaccggca acaacgtgcg ctccaactca cggcggccga gcatgtagag cacgtacgac
     1501 tggtctttgg ccgcgtcttc gccgcgcgcc aacatcggcc cgtcgggcgt cgtgacgacg
     1561 cgcgcgtggt ggccggtggc gactgcgtcg aagcccagct gctcggcgcg cgcgaggagc
     1621 cggccgaact tcatcgtgcg gttgcactcg acgcacgggt tcggcgtgcg gccggcctcg
     1681 tacgcggccg tgtacggcgc gacgacgtgc tcgtcgaacg cgtcggtgaa gttgaagacg
     1741 gagtgcggga tgtcgagctg cgcggcgacg cggcgagcgt cctcgacgtc ggcaacgctg
     1801 cagcagccgg agtcactgac gccgccccag agcttcaacg tcacgcccgt gacctcatgg
     1861 ccctgccgga gcaacagcgc ggccgcgacc gacgaatcga cgccgccgct catcatcgcc
     1921 atgaccttca tgacgcgccc ctcacgccgc acgcagcttg gcgacgactt cgggcacgac
     1981 cgtcagcgcg cgctcgatct cgtcgtcggt cgacgcgtac ccgaggctga accgcaccga
     2041 ccgcaacgcg cgctcgcgcg ggatacccat cgcgatgagc acatgcgacg ggtcgaccgc
     2101 acccgagctg cacgcggagc cggacgcggc gaacacaccg gcctggtcga gcgcgacgag
     2161 caacgtctcg gcctcgacgt cggggatcgc cacgtgcagc agcccctcga cgcggcgcgc
     2221 gggatcgcca ttgacgacga tgccgtcgat cgcggcgagc agtcccgcct cgaaacggtc
     2281 gcgcaatgca gcgatgcgcg cggtctcggc cgcgcgctgt tcgtgggtga tgcgcagtgc
     2341 cgtcgcgaac gcgacgatgc cggcgacgtt ctgggtaccg gcccgcaggt cgcgttcgtg
     2401 cccgccccct tcgacgagcg ggaccagcgc gacatcgtcg cggacgacga gcgcgccgac
     2461 gcccttcggt ccgccgaact tgtgacccga gatcgcgacc agctgcgcac cggatgtcgc
     2521 aatccgcagg tcgcgccact gcggcgcctg caccgcgtcg gtgtgcaaca ccgcatgggg
     2581 cgcgtgctcg cggacgaggg ccgcgaccgc gtcgagcggc tgttgcacgc cggtctcgtt
     2641 gttgacggtc atcaccgaca cgaccgccgt gcggtcgtcg agcgcaccgg ccagcgcgtc
     2701 gaggtcgacc gcgccgtcga gcccggcgcc gacggtcgtg acccggaagc cgtcgcggcc
     2761 gagacgggcg caggcgccga gcacggcctt gtgctcgatg ccggtggtga ccacgccgtc
     2821 gaggcccgac cgctccc
//
LOCUS       DASUGA010000759         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_502588, whole genome shotgun sequence.
ACCESSION   DASUGA010000759 DASUGA010000000
VERSION     DASUGA010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502588"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="VFX21_14930"
     CDS             <1..1042
                     /locus_tag="VFX21_14930"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5097319.1"
                     /translation="QIDAGTQPTDASARRAFDLQADAFGPGFDGPLVVVMQDRDGVKL
                     ADATQLADAIGRDDAIAAVARPTRNEAGTVAVVQAFSRYAPHDERTAQLVDRIRDRVV
                     PLTTTGNTRVLVTGSTAANVDLADQMQQRLPWIIGSVIAVSFVLLMIAFRSLLVPLKA
                     ALMNVLSIGAGYGVVVAVFQWGWGRDIVGLRETVPIVSWVPMLMWAILFGLSMDYEVF
                     LISSIRHAFRRHGDNRAAVVEGLGSTGRVITAAALIMLTVFGSFVAYPDPVVKMIGLG
                     LAVAIFVDATVVRMLLVPATMTLLGRANWWLPRRLARVLPSVDLDSTETPERVALVAD
                     GGERREPVAIGS"
     gene            1070..1438
                     /locus_tag="VFX21_14935"
     CDS             1070..1438
                     /locus_tag="VFX21_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097320.1"
                     /translation="MRELFTRSAVPATSPAVAGTAQVTQPKLPFACDDRAWFCLHTWS
                     GERAHDSWANVIASVIESLQGGPVDIRRRDGEWVRVTVPTVPNDWGWRELQRIEDCVR
                     LEPVGWVPLDDIVAVHPATP"
     gene            complement(1442..1681)
                     /locus_tag="VFX21_14940"
     CDS             complement(1442..1681)
                     /locus_tag="VFX21_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097321.1"
                     /translation="MKTMTCKQLGGPCDLELHGETADEVIKAQDRHLNEVVAAGDDTH
                     AVALKEMKGRWKRPVKGMGWYKATKRAFAELPEDN"
     gene            1743..>2130
                     /locus_tag="VFX21_14945"
     CDS             1743..>2130
                     /locus_tag="VFX21_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5097322.1"
                     /translation="MAEAVKREQARTRFARRAVVDAARVLFADRGYAATTVDAISERA
                     DVPIATVYRLFSSKLGILKALLDTSIAGDDQPVAVPDRPDIASVFAARDPAKLLTGSA
                     GVTTAINERTNDVYFVLVSAAASDPGA"
BASE COUNT          309 a          728 c          697 g          396 t
ORIGIN      
        1 gcagatcgac gcggggacgc aaccgacgga cgccagcgcc cggcgcgcgt tcgaccttca
       61 ggccgacgcg ttcggccccg ggttcgacgg tccacttgtc gtcgtcatgc aagaccgaga
      121 cggcgtaaag ctcgccgacg ccacccagct cgcggatgcg atcggtcgcg acgacgcgat
      181 cgcggcagtc gcgcgaccca caaggaacga agcgggcacc gtcgccgtcg tgcaggcgtt
      241 ctcgcgctac gcaccccacg acgaacgcac tgcgcagctc gtcgatcgca tccgcgatcg
      301 tgtcgtgcct ttgaccacga ccggcaacac acgtgtcctc gtcactggct cgacggcggc
      361 caacgtcgac ttggccgacc aaatgcaaca gcggttgccg tggatcatcg gctcggtcat
      421 cgcggtgtcg ttcgtgttgc tgatgatcgc gttccgctca ctgctggtgc cgctgaaggc
      481 agcgttgatg aacgtgcttt cgatcggcgc cggatacggc gtcgtggtcg ccgtgttcca
      541 gtggggctgg ggcagggaca ttgtgggcct gcgcgagacc gtcccgatcg tgtcgtgggt
      601 cccgatgttg atgtgggcga tcttgttcgg cctttcgatg gattacgagg tgttcctgat
      661 ctcgagcatt cgccatgcgt tccgtcgtca cggcgacaac cgtgccgccg ttgtcgaagg
      721 tctcggatcg accggtcgcg tcatcacggc cgccgcactc atcatgctca cggtgttcgg
      781 gtccttcgtt gcgtatcccg atccggtcgt aaagatgatc ggcctcgggc tcgcggtcgc
      841 gatcttcgtg gatgcgaccg tcgttcgcat gttgctcgtc ccggcgacga tgacgctgct
      901 cggccgcgcg aactggtggc ttccccgccg gctcgcccgc gtgttgccga gcgtcgattt
      961 ggattcgacc gagactcccg aacgtgtcgc actcgtcgcc gacggcggcg agcgtcggga
     1021 accggtcgcg atcgggagct gagctcgctt tcgagaaagg agcgcggccg tgcgcgagct
     1081 cttcacccga tcggcggtcc cggcgacgtc ccccgcagtc gccgggaccg cgcaagtcac
     1141 ccagccgaaa ctgccgttcg catgcgacga tcgcgcgtgg ttctgtctac acacgtggtc
     1201 gggcgagcgc gctcatgact cgtgggccaa cgtgattgcg tccgtcatcg agtcgctcca
     1261 aggcggaccc gtcgacattc gacggcgcga cggcgagtgg gttcgcgtga cggtgccgac
     1321 ggtgccgaac gactggggat ggcgggaact gcagcgcatc gaggactgcg tccgactcga
     1381 gcccgtcggt tgggtgcccc tcgacgacat cgtggccgtg cacccggcga cgccgtgagg
     1441 ctcagttgtc ttcgggaagt tcggcgaacg cgcgcttggt cgccttgtac cagcccatcc
     1501 ccttcacggg gcgcttccac cggcccttca tctccttgag cgcgacagcg tgcgtgtcgt
     1561 cgccggccgc gacgacctcg ttgaggtggc gatcctgggc cttgatcacc tcgtccgcgg
     1621 tctcgccgtg caactccagg tcgcacggcc cgccgagttg cttgcacgtc atcgtcttca
     1681 tcaggggctc ctctcgcctc tcgatagtcg tactctcgaa ctgagagtga tactatcgat
     1741 ttatggccga ggccgtcaag cgcgagcaag cccgcacccg gttcgcccgg cgcgcggtcg
     1801 tcgacgcggc ccgcgtgctg ttcgccgacc gcggctatgc ggcaacgacc gtcgacgcca
     1861 tcagcgagcg cgccgacgtg ccgatcgcga cggtgtatcg cctgttctcg tcgaagctcg
     1921 gcatcctcaa agccctgctc gacacgtcca tcgccggtga cgaccagccg gtcgcggttc
     1981 ccgaccgacc cgacatcgcg tcggtgttcg cggcacgcga tccggcgaag ctcctcaccg
     2041 gcagcgccgg tgtcacgacg gccatcaacg aacggacgaa cgacgtctac ttcgtcctcg
     2101 tgagcgcggc cgcgtccgat cccggcgccg
//
LOCUS       DASUGA010000760         4542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1651171, whole genome shotgun sequence.
ACCESSION   DASUGA010000760 DASUGA010000000
VERSION     DASUGA010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651171"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VFX21_14950"
     CDS             <1..585
                     /locus_tag="VFX21_14950"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5097323.1"
                     /translation="LAEAVEILRVLWTGDTVDYDGDFYKVVNARVFDPPDHDLSIIVS
                     AFGAQAAELAARVGDGLWTSGPDSKLIDQFHTVGGKGPVYGQMHVCFGPDEDACRKIV
                     HDVWPNAAFPGQLAQELPTWTHFEQVAELVTPEKAAEHIPCGPDIEPLLDSVRKYLDA
                     GYTDIYFHQIGPDQDALFAAWESELADALREITD"
     gene            1074..2306
                     /locus_tag="VFX21_14955"
     CDS             1074..2306
                     /locus_tag="VFX21_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEX5097324.1"
                     /translation="MPDFVSPAWMPSSLREAAVAGDATPDDLASGAAWSHLLTKLQAA
                     SATVLANPASRNPVDMAAGLRHLLVLLWIGIDDALRRDSGAVLAVRPSNTDAVFKWGM
                     DCPDCLYTGAGLRGGETYRLWGNRGTARYVGLQTMAGMASTANVLLDEIELEPNGDFE
                     IILSADAHDGNWMQIADDATQLVVRHFFYDWDTEVASSLSIERLSGPRPAPRRSEPID
                     PRAAMARQIVALGDFVEANLDFFLQFATPERPNTFNPPFDGTAMGAAAENRPVIGSFL
                     LGPDEALVVEVEPPEGLYWSYSIGNPWWETIDYARHQSSLNGHQAEIDADGMLRVVIA
                     HEDPGIANWLDTAGHGEGPVILRCVRTETAPVPTTRAVPFAEVANALPAGARRISPAE
                     RATVMDARARGVARRFVR"
     gene            2303..3448
                     /locus_tag="VFX21_14960"
     CDS             2303..3448
                     /locus_tag="VFX21_14960"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5097325.1"
                     /translation="MTLDADELEAAAREETGLTDFGDGLHRDGLERLVASMNEEGDLT
                     ETGEMMQRIRLGAHLTARLRVEQTYREHPEIEEQVVEGPVFVIGLPRTGTTALSHLVA
                     ADPQFRSLRLWESGAPVPPPEAATQHTDPRIAETEIGLALMNEAFPLMQTMYHSEATT
                     ATECQDLMGMSFRTAHFDGSARVPSYMDWVVDCDMRGTYEYHRRVLRLLQWHCPPRLW
                     HLKTPVHMFALDALIEAYPDARFLWSHRDPAKVLGSVCSLVHYTRSWSSDRDDAFELG
                     REMVDRWALAVERAMEFRARVGDERFADVPFAALQRDPVDAIAASYERIGLELSDESR
                     RAITQWATGHQPGSHGSHRYDLADFGIDAERVRARFARYIAEYAATD"
     gene            complement(3494..>4542)
                     /locus_tag="VFX21_14965"
     CDS             complement(3494..>4542)
                     /locus_tag="VFX21_14965"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5097326.1"
                     /translation="LVSTPRAYGWPWDVAAVTGAGYGDLDLDTAERVLGDDADVESWT
                     ALGLLNELSLNGQPMMSVIGFDRASDVDLPVLDGALPRGVDQVAIGAAVARAMNLAVG
                     DAVDLGGAFAGRRATVSGIVVFPTLGPFMADRVGAGTGLLVSETAFEDVRGPYANADV
                     RDLATFVGVKLRDGTRHVATVERVRAGLATLDKYDSPVFDYPTPVRPPSIIDTRSTRV
                     VAVTVGIAFAAVTTIGLMSNAWSSSRGRRRELSVLRALGCRNSEVRRSVYVQSVGTMA
                     GALVLGVPLGVIVGRLLWRGFADELGVVPDPASPWPAVFATVAGGLAVAVMAAWIPAR
                     FATRSSPATSLSAE"
BASE COUNT          740 a         1551 c         1558 g          693 t
ORIGIN      
        1 ctggcggagg cggtcgagat tctgcgcgtc ctgtggaccg gcgacacggt cgactacgac
       61 ggcgacttct acaaggtcgt gaacgctcgc gtgttcgatc cgcccgacca cgatctttcg
      121 atcatcgtgt ccgccttcgg ggcgcaggcc gccgagttgg ccgcgcgcgt cggtgacggg
      181 ctctggacga gcggccccga ttcgaagctc attgatcagt tccatacggt cggtggcaaa
      241 ggccctgtgt acgggcagat gcatgtgtgt ttcggtcccg acgaggatgc gtgccgcaag
      301 atcgtgcacg acgtctggcc gaacgcagcg tttcccggcc agctcgcgca ggagttgccg
      361 acctggacgc acttcgaaca ggtcgccgag ttggtgacac ccgagaaggc agccgagcac
      421 atcccgtgcg ggcctgacat agaaccgctg ctcgattcgg tgcgcaagta tctcgacgcc
      481 ggctacaccg acatctactt ccaccagatc ggccccgatc aggatgccct attcgccgcg
      541 tgggagtcgg aactcgccga tgcactgcgt gagatcacgg actgatcaac gcggtgcgac
      601 caacccgcaa acgcaacgcg gtgcgccaac tgagcaaacc atccgtactg ggcagcaatg
      661 cacggacgca atcgacgttc ggcaggggat cgggcgtgtt cgagccgtcg cgcgtcgcca
      721 tcgcctatgc gctgttcgac cccggcgagc cggtcggcgg tgacaatcac cgtgacaatc
      781 aaactcggcg gtgctgtgca gactccgtgg aatcggtgga cgcaatagac cgatgggact
      841 gagaggagtg cgccgcatgg acgtgctgga cggttgtcgt gcgactcata atccctcggt
      901 cgaaggttcg atcccttccc ggcccacgcc gaatgtctca agcccgtgtc agcacggttg
      961 atgcggcagc ccgggttggt tcacgtcgct gcccagcgcc cggcggtaac cggcgagctt
     1021 atgctccctc gatctgcgcg gcgtgggtcg cgcccgagtc ccggagctgc cgcgtgcccg
     1081 atttcgtgag tccggcatgg atgccgtcgt cgttgcgcga ggcggccgtc gccggtgacg
     1141 cgacgcccga cgacctcgcg tcgggcgccg cgtggtccca cctgctcacc aagttgcagg
     1201 ccgcgtcggc gacggtgctc gcgaacccgg cgtcccgtaa cccggtcgac atggccgccg
     1261 gtctgcgcca cctgctcgtg ctgctgtgga tcggcatcga cgacgcgctg cgccgcgact
     1321 cgggtgcggt gctcgccgtg cggccgtcga acaccgatgc ggtcttcaag tggggaatgg
     1381 actgtcccga ctgcctctac accggcgccg gtctccgggg cggcgagacc taccgcctct
     1441 ggggcaaccg cggcacggcg cgctacgtcg gactccagac gatggccgga atggcgagca
     1501 ccgcaaacgt tctcctcgac gagatcgagc tggaaccgaa cggcgacttc gagatcattc
     1561 tttcggccga tgcgcatgac gggaactgga tgcagatcgc ggacgacgcg acgcagctcg
     1621 tcgtgcgcca cttcttctac gactgggaca ccgaggttgc gtcgtcattg tcgatcgagc
     1681 ggctctccgg tccccgaccg gcgccgcggc ggtcggagcc catcgacccg cgcgcggcga
     1741 tggcgcgtca gatcgttgcg ctcggcgact tcgtcgaggc caatctcgac ttcttcttgc
     1801 agttcgcgac gcccgaacga ccgaacacgt tcaacccgcc gttcgacggc accgcgatgg
     1861 gcgcggccgc cgagaaccgg ccggtgatcg gctcgttcct cctcggaccc gacgaggcgc
     1921 tcgtcgtcga ggtggaaccg cccgagggtc tgtactggag ctactcgatc gggaacccat
     1981 ggtgggagac gatcgactac gcgcgccatc agtcgagcct gaacggtcac caggcggaga
     2041 tcgacgccga cggaatgctg cgcgtcgtga tcgcgcacga ggacccgggt atcgcgaact
     2101 ggctcgacac ggccggccac ggcgaaggac ccgtgatcct gcgctgcgtg cgcaccgaga
     2161 ccgcgcccgt gcccacgacg cgcgccgtcc ccttcgccga agtcgcgaac gcgctcccgg
     2221 cgggtgcgcg ccggatctcg cccgcggaac gcgcgacggt gatggatgcg cgagcgcgcg
     2281 gcgtggcgcg gcggttcgtc cgatgacgct cgacgccgac gagctggaag cggcggctcg
     2341 cgaagagacg ggactcaccg acttcggcga cggcttgcac cgcgacggac tggaacggct
     2401 cgtcgcgtcg atgaacgagg agggcgacct gaccgagacc ggcgagatga tgcaacgcat
     2461 tcgtctcggc gcacacctca ccgcccgcct gcgcgtggag cagacctacc gcgagcaccc
     2521 cgagatcgag gagcaggtcg tcgagggccc ggtgttcgtg atcggcctgc cccgtacggg
     2581 gacgacggca ctgagtcatc tcgttgcggc cgatccgcag ttccggtcgc tgcgactgtg
     2641 ggagtcgggc gcaccggtac caccaccgga ggccgcgacc cagcacaccg atccgcgcat
     2701 cgccgaaacc gagatcggcc tcgcgctgat gaacgaggca ttcccgctca tgcagacgat
     2761 gtaccactcg gaagcaacca ccgcgacaga gtgtcaggac ctcatgggta tgagcttccg
     2821 caccgcgcac ttcgacggct cggcgcgggt gccgtcgtac atggactggg tcgtcgactg
     2881 cgacatgcgg ggtacgtacg agtaccaccg ccgtgtgctg cgcctgctgc agtggcactg
     2941 cccgccgcgt ctgtggcacc tcaagacgcc agtccacatg ttcgcgttgg atgcgctcat
     3001 cgaggcgtac cccgacgccc ggttcctgtg gagccaccgg gatccggcca aagtcctcgg
     3061 ttcggtgtgc agcctcgtcc attacacgcg cagctggagc agcgaccgtg acgacgcgtt
     3121 cgagctcggc cgagagatgg tcgaccgttg ggcgctcgcc gtggagcggg cgatggagtt
     3181 ccgagcgcgc gtcggcgacg agcggttcgc cgacgtgccg ttcgcggcgc tgcagcgcga
     3241 ccccgtcgac gcgatcgccg cgagctacga acggatcgga ctcgagctgt ccgacgagtc
     3301 ccgtcgggcg atcacgcagt gggcgacagg tcatcagccg ggatctcacg gctcgcaccg
     3361 gtacgacctt gcggacttcg ggatcgatgc cgagcgcgtc cgggcgcggt tcgcgcgcta
     3421 catcgccgaa tatgccgcga ccgactgacg gtgttcgcgc tcgctgcgcc ccgggttcgc
     3481 ccagtccgga tggtcactct gcgctgaggc tcgttgccgg gctcgaccgc gtggcgaagc
     3541 gcgccgggat ccacgccgcc atcacggcaa ccgcgagtcc gccggcaacc gtcgcgaaca
     3601 cggcgggcca aggacttgcg gggtcgggga cgacgccgag ctcatcggcg aatccccgcc
     3661 acaggagtcg accgacgatc acgccgagcg ggacgccgag cacgagtgcg ccggccatcg
     3721 tgcccaccga ctgcacgtac accgagcgcc ggacctccga gtttcgacag ccgagcgcgc
     3781 gcaacaccga gagctcgcgg cggcggccgc gtgaggagga ccatgcgttc gacatcaggc
     3841 cgatcgtggt caccgctgcg aacgcgatgc ccactgtgac ggcgaccacg cgagtggagc
     3901 gcgtatcgat gatcgacggc gggcgaaccg gggtcgggta gtcgaacacc ggagagtcgt
     3961 acttgtcgag tgtcgcgagt ccggcgcgga cgcgctcgac ggtggcgacg tggcgcgtgc
     4021 cgtcgcggag cttgacgccg acgaacgtcg ccaggtcgcg aacgtcggcg ttcgcgtacg
     4081 gcccgcgtac gtcttcgaac gccgtctcgc tgaccaggag cccggtaccc gcaccgacgc
     4141 gatcggccat gaacggtccg agggtgggga agacgacgat gcccgacacg gtcgcgcgtc
     4201 tccctgcgaa cgcgccgccg aggtcgacgg catcgcccac ggcgagattc atcgcccgtg
     4261 cgaccgccgc gccgatcgcc acctggtcga cgccgcgcgg gagcgcacca tcgagcacgg
     4321 gcaggtcgac gtcggaggca cgatcgaagc cgatcaccga catcatgggc tgcccgttga
     4381 gcgacagctc gttcagcagt cccagcgcgg tccacgactc gacgtcggca tcgtctccga
     4441 ggacgcgctc ggccgtgtcg aggtcgaggt cgccgtagcc ggcgccggtg accgccgcga
     4501 cgtcccacgg ccatccgtac gcgcgcggcg tcgataccaa gg
//
LOCUS       DASUGA010000761         3269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_225107, whole genome shotgun sequence.
ACCESSION   DASUGA010000761 DASUGA010000000
VERSION     DASUGA010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225107"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VFX21_14970"
     CDS             <1..326
                     /locus_tag="VFX21_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX5097327.1"
                     /translation="DVELADGTADDVYLAELAAALPRVLAASAPDLVVYIAGADPHEG
                     DRLGRLALTFDGLARRDAMVLDLCREVGIPVAITIGGGYGRRIEDTVAAHLRTVRVAR
                     AHARD"
     gene            512..>3269
                     /locus_tag="VFX21_14975"
     CDS             512..>3269
                     /locus_tag="VFX21_14975"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEX5097328.1"
                     /translation="MPQLAPTFRRLTFLFALPLAFTSVHAQQPTQQRPDTITGRRTQH
                     TIPVPTAVASPRVGNVTLDGRLDDEAWSKATPITEFTQVDPKEGEPGTQRTDVRFVYD
                     AEALYVGARMYEKDPKDIVTRLVRRDADMESDYLEVVIDAYHDHLGRAFFDVNPSGVK
                     FDALGVGTSNPDGSWDAIWEAATKIDSLGWTAELRIPYSQLRFSRSEVQTWGLQVRRY
                     MQRSHEYTQWSHWKKTEIGGPSRFGHLEGIRIGSVPKHLELLPYAVTRSRHIRPDTPG
                     DPFNDGSRQDMRFGGDVKALITSNLTLDATINPDFGQVEVDPATVNLSAFETFFEEKR
                     PFFVAGAGIFNFGNASCYFCSNFSSIESFYSRRIGRAPQAAGLAYDAGQYADVPENST
                     ILGAAKVTGRTSSGVTLGLLNAVTRREMASVVGEDGTRLRQEVEPLSNYFVGRATKDY
                     RDGNLVVGAIGTSVVRRLDDPALADRLTSHAEAVGGDMVLTWDKKNYQLLASAMLSNV
                     SGDSAAMMRVQQSSAHYFQRPDRAAGDEGWFRSGILDASYRPGATDLRGLATYLRMAK
                     DGGSFNWEAQANVRTPGFEVNDISFLSRADYAQFVGNVAYGWTKPTRWYRDAAIVFGA
                     QQSQNFDGDLTNRDVHIYVQSQTPQFWRFNLWTLHNFQVYDDRLLRGGPVAVLPAGDV
                     YSANLNSDSRKGVVFNLNPRYQRNREGGFQSAIFTSVRWKPVSNVSLSLGPSYNFTRG
                     IQQYVTTVDDATNAAFGGERYVFSSLVQKTVSMDTRLAVTFTPNSSLELYAQPFIATG
                     NYSDFKEFDSPRQLRKSVYGRDRGTISATRDLDGLVTGYTVDPDGNGAAPAFSFDNPS
                     FDARSLIGNVVYRWEYRPGSTLYLVWTQSRSDELTDVGTLDFARDRRALFSTHPDNIF
                     LVKLN"
BASE COUNT          542 a         1197 c         1011 g          519 t
ORIGIN      
        1 tcgacgtcga gctcgccgac ggcacggcgg acgacgtcta tctcgctgag ctcgcggcgg
       61 cgctgccacg cgtgctcgcg gcgtcggcgc ccgatctggt cgtctacatc gccggcgccg
      121 atccacacga aggtgaccgg ctcggccgtc tcgcgttgac cttcgatggg ctcgcgcgcc
      181 gtgacgcgat ggtactcgac ctgtgtcgcg aggtcgggat cccggtcgcg atcaccatcg
      241 gcggcggcta tggccgccgc atcgaggata ccgtcgcggc gcatctgcgc accgttcggg
      301 tcgcgcgcgc gcacgcgcgg gactgagcgc aggatcgctc aatccccgcg cgggggatca
      361 gcccaccttc acggatgccg tgaccgcgcc ccgccgcgcc agagttgttt cacacgacgc
      421 catcaccgtc gccgacggaa ccaatctccg tcggaccgtg tccaatgagg gttccaccag
      481 agtgccctct ctctccccac caacatcgca cgtgccgcag ctcgccccca ccttccgtcg
      541 tctcaccttc ctcttcgccc tgccgctcgc gttcacgagc gtccacgcgc agcagccgac
      601 gcagcaacga ccggacacga tcacgggccg tcggacccag cacaccatcc ctgttcccac
      661 cgcggttgcg tcgccgcgcg tcggcaacgt caccctcgac ggccgtctcg acgacgaagc
      721 ctggtcgaag gcgaccccca tcacggaatt cacccaggtc gatccgaagg aaggagagcc
      781 tggcacccag cgcaccgacg tgcgcttcgt gtatgacgcg gaagcgctgt acgtcggcgc
      841 gcgcatgtac gagaaggatc cgaaggacat cgtcacgcgc ctcgtccgtc gcgacgccga
      901 catggaatcg gactacctcg aggtcgtgat cgacgcgtac cacgaccacc tcggccgcgc
      961 cttcttcgac gtcaacccgt ccggcgtgaa gttcgacgcc ctcggcgtcg gcacgtcgaa
     1021 tcccgacgga tcgtgggacg ccatctggga ggcggcgacg aagatcgatt cgctcggctg
     1081 gaccgccgag ctgcgcatcc cgtactcgca gctgcgcttc tcgcgctccg aggtacagac
     1141 gtgggggctc caggttcgcc gctacatgca gcgctcgcac gagtacacgc agtggtcgca
     1201 ctggaagaag accgagatcg gcgggccatc gcggttcggc cacctcgagg ggatccgcat
     1261 cggcagcgtg ccgaagcatc tcgagctgct gccgtacgcc gtcacccgct cgcggcacat
     1321 ccgccccgac acgccgggcg acccgttcaa cgatggaagc cggcaggaca tgcgcttcgg
     1381 cggcgacgtg aaggcactga ttacctccaa cctcactctc gacgcgacca tcaaccccga
     1441 cttcggccag gtcgaggtcg atccggcgac ggtgaacctg agcgcgttcg agaccttctt
     1501 cgaggagaag cgccccttct tcgtcgccgg cgcgggaatc ttcaacttcg gcaacgcgag
     1561 ctgctacttc tgcagcaact tcagctcgat cgagtcgttc tactcgcgtc gcatcggccg
     1621 cgcgccacag gccgcgggcc tcgcgtacga cgcggggcag tacgccgacg tccccgagaa
     1681 ctccacgatc ctcggcgcgg cgaaggtcac cggccgcacg agctccggcg tgacgctcgg
     1741 cctgctgaat gcggtgacgc ggcgcgagat ggcgagcgtc gtgggcgagg acggcacgcg
     1801 cctcaggcag gaggtcgagc cactgtcgaa ctatttcgtc ggccgagcga cgaaggacta
     1861 tcgcgacggc aatctggtcg tgggcgcgat cgggacctcg gtggtgcggc gcctcgacga
     1921 tcccgcgctg gccgatcggc tcacctcgca cgccgaagcg gtcggcggcg acatggtgct
     1981 gacgtgggac aagaagaact atcagctgct cgcgtcggcc atgctgtcga acgtgtcggg
     2041 cgactctgcc gcgatgatgc gcgtacagca atcgagcgcg cactacttcc agcgccccga
     2101 ccgcgcggcc ggcgacgagg gttggttccg cagcggcatt ctcgacgcgt cataccgtcc
     2161 cggcgcgacc gacctgcgcg ggctggcgac ctaccttcga atggccaagg acggcggctc
     2221 cttcaactgg gaagcgcagg cgaacgtccg cacgccgggc ttcgaggtca acgacatcag
     2281 cttcctctcg cgcgccgact acgcgcagtt cgtcggcaac gtcgcctacg gctggacgaa
     2341 gccgacgcgc tggtaccgcg acgcggcgat cgtgttcggc gcacaacagt cgcagaactt
     2401 cgacggcgac ctgaccaacc gcgacgtcca catctacgtg cagagccaga ccccgcagtt
     2461 ctggcgcttc aatctctgga cgctgcacaa cttccaggtg tacgacgacc gcctgctgcg
     2521 cggcggcccg gtcgccgtcc tccccgccgg cgacgtgtac tccgcgaacc tcaacagcga
     2581 ctcgcgcaag ggcgtcgtct tcaacctgaa cccgcgctac cagcgcaacc gcgagggcgg
     2641 cttccagtct gccatcttca cgtcggttcg ctggaagccc gtgtccaacg tgtcgctctc
     2701 gctcgggccg tcgtacaact tcacgcgcgg catccagcag tacgtcacga cggtggacga
     2761 cgcgacgaat gccgcgttcg gcggggagcg ctacgtcttc tcctcgctcg tgcagaagac
     2821 cgtgtccatg gacacgcgcc tcgccgtcac cttcaccccg aactcctcgc tcgagctgta
     2881 cgcgcagccg ttcatcgcga cgggcaacta ctccgacttc aaggagttcg actcgccgcg
     2941 gcagctccgg aagagcgtct acggccgcga ccgcggcacg atcagcgcca cgcgggacct
     3001 cgacggcctg gtcaccggct acacggtgga ccccgacggc aatggcgctg ccccggcgtt
     3061 ctccttcgac aacccgagct tcgacgcgcg ctccctcatc ggcaacgtgg tctaccgttg
     3121 ggagtaccgc cccggttcca cgctgtacct ggtctggacg cagtcgcgca gcgatgagct
     3181 caccgacgtc ggcacactcg acttcgcccg cgaccgccgc gccctcttct ccacgcatcc
     3241 cgacaacatc ttcctggtca agctgaatt
//
LOCUS       DASUGA010000762         2664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1058034, whole genome shotgun sequence.
ACCESSION   DASUGA010000762 DASUGA010000000
VERSION     DASUGA010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058034"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VFX21_14980"
     CDS             complement(<1..622)
                     /locus_tag="VFX21_14980"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5097329.1"
                     /translation="MLAALRHRDVRLLMSSSAVSEVGDWLYNVALAVYVYEQTHSSAW
                     IGIITLLRLIPYVLLAPLGGVIADRYERRTVLVVSDVLRAGFMALLALAVAGGMPVIV
                     VGLLATATTAAGTSSLPATVAMLPAMLDEDELGAANSLTSLIQSVAVVSGPALGALLL
                     VFAAPTWSFVVNAASFALGAVLTWRISTRSRPDRREHGDTHPVREFA"
     gene            1237..1392
                     /locus_tag="VFX21_14985"
     CDS             1237..1392
                     /locus_tag="VFX21_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097330.1"
                     /translation="MPRQHTYDDDPRIHDARVDDPGIHNAWFDHAWFDHAGIHHAGKH
                     DFDVHLA"
     gene            complement(1720..>2664)
                     /locus_tag="VFX21_14990"
     CDS             complement(1720..>2664)
                     /locus_tag="VFX21_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012180967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX5097331.1"
                     /translation="GASTADLALILVDARKGVIEQSRRHSFLASLLGVPHLVLCVNKM
                     DLVDYDQATFEKIKSEFKDFAAKLDVHDLTFIPISALHGDNVVERSANMPWYEGSSLL
                     HQLEDVHIASDRNLIDCRFPVQYVIRPRDDAHHDYRGYAGQVASGTYKVGDDVVVLPS
                     GFTSKIAAIDGPDGPVDEAVPPMSVTIRLEDEIDISRGDMICRPHNQPQVGQDVDAML
                     CWFSERAQLQPGGKYALKHTSKWARALVKDLHYRLDVNTLSRDESAQSLALNEIGRVT
                     LRTTLPLFYDDYRRNRVTGSFVLVDEATNDTLAGGMIL"
BASE COUNT          493 a          868 c          874 g          429 t
ORIGIN      
        1 cggcaaactc acgcacggga tgcgtgtcgc cgtgttcgcg ccgatcgggc cggctgcgag
       61 tggagatgcg ccacgtgagg acggcaccga gcgcaaacga agcggcgttc acgacgaacg
      121 accatgtcgg cgcggcgaag accagcaaga gcgcgccgag cgccggcccc gaaaccaccg
      181 ccacactctg aatgaggctg gtcagactgt tggcagcacc gagctcgtcc tcgtcgagca
      241 tggcggggag cattgcgacc gtcgccggca gggacgaggt gcccgctgcc gtcgtggctg
      301 tagcgagcaa accgacgacg atgaccggca tgccgcccgc gaccgcgagt gcgagcaagg
      361 ccatgaaccc ggcccgaagg acatccgaaa cgacgaggac cgttcgccgc tcgtaccggt
      421 cggcgatcac gccaccgagg ggagcaagca gcacgtacgg aatcagtcgg agcagggtga
      481 tgatgccgat ccatgcactc gagtgcgtct gttcgtagac atagacggcg agcgcgacgt
      541 tgtagagcca gtcaccgacc tccgaaaccg cggacgacga catcagcaga cgaacgtcgc
      601 ggtgccgcag cgccgccagc atccccccgg accggtgcac gcacgattgt ggcagcacat
      661 cggagcgttg aaggaagttc gacgcggtcc gtaggttttt tctgcgcgtg tcctccctcg
      721 ttgggcggct tgtcgaaatt gcccgtttga ccggtaccac aaagcgcggt cgaatcccgc
      781 tcgattcagg ggggcattga tgcgggagaa tcgccgaact gagttgcgtg cgctgcatcg
      841 gcgcgtgtgg ggaccgtcga cgtaccgcgc ctatgtgcgc tccgtacgtc ggcggcgcgc
      901 gggtgtggtt gcggccgggg cggccgccgc gttcgcaatg ttgttcgtca tgctcggtgc
      961 cattccggac gcgagtgcga cgaagccgaa ccctgagcac aaggtgaccc tgtgtcaccg
     1021 cacggatagc tacacgaatc cctacgtcgt catcacggtc gacatcgcaa gcgtgaagtt
     1081 cgccgggcac aacggccaca acgggcccgt tttcagtcct tcgttgccga agcacacgaa
     1141 gtggggagac atcattccgc ccttcgacta cggacccgga cgccaatacg ccggcaagaa
     1201 ctggacgcag gaaggcatgg cgatcttcgc caaccagtgc cgcgccagca cacctacgac
     1261 gacgaccccc ggatccacga cgcccgcgtc gacgaccccg ggatccacaa cgcctggttc
     1321 gaccacgcct ggttcgacca cgccgggatc caccacgccg gaaagcacga cttcgacgtc
     1381 catctcgcct gattcgacga ccacaacgct tccggggacc atcccgaccg tcgcgccgaa
     1441 cgaaagcacc tctaccaccg tcgatgcgaa caccgcggcg ggcggcacga cgacgtctac
     1501 gagcgtttcg ccgacgagtg caacacaagg accgcttccg gtgaccggca ccgccgctct
     1561 cgggctcgcg ctcctcggac tcgcttccgc cgcaacagga gtcgtactcc ttcgtcagcg
     1621 agccacccgc gagagctagc aagcacggtc gctgtccatt ggaagttgag ccaccgaatc
     1681 ggtggctgga ttgccggaga accgtcgatg ctgccgctgt tagaggatca tgccgccggc
     1741 aagcgtgtcg ttcgtggctt cgtcgaccaa cacgaaggag cccgtgacgc ggttacggcg
     1801 atagtcgtcg tagaagagcg ggagcgtggt gcggagcgtg acgcgcccga tctcgttcaa
     1861 cgcgagggac tgcgctgatt cgtcgcgcga aagcgtgttg acgtcgagcc ggtagtgcag
     1921 gtccttcacg agcgctcgcg cccacttcga cgtgtgcttc aacgcgtact tgccgccggg
     1981 ctggagctgc gcgcgctcgc tgaaccagca gagcatggcg tcgacgtcct ggccgacctg
     2041 cggctggttg tgcggacggc agatcatgtc gccccggctg atgtcgatct cgtcttcgag
     2101 tcggatcgtc accgacatcg gcggcacggc ttcgtccacg ggaccgtcgg ggccgtcgat
     2161 tgccgcgatc ttcgacgtga agcccgacgg caacaccacg acgtcgtcgc cgactttgta
     2221 ggtgccggac gcgacctggc ccgcgtagcc gcggtagtcg tggtgcgcgt cgtcgcgagg
     2281 ccggatgacg tactgcaccg ggaaccggca gtcgatgagg ttgcggtcgg aggcgatgtg
     2341 cacgtcttcg agctggtgca gcagcgatga gccctcgtac cacggcatgt tcgccgaccg
     2401 ctcgacgacg ttgtcgccgt gcaacgcgga gatcggaatg aacgtgaggt cgtggacgtc
     2461 gagcttggcc gcgaaatcct tgaactcgct cttgatcttc tcgaacgtcg cctggtcgta
     2521 gtcgacgagg tccatcttgt tgacgcacag caccaagtgc ggcacgccca acagcgacgc
     2581 gaggaacgag tgccggcgcg attgctcgat cacgcccttg cgcgcgtcca cgaggatcag
     2641 cgcgagatcg gcggtcgacg cgcc
//
LOCUS       DASUGA010000763         2053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_780479, whole genome shotgun sequence.
ACCESSION   DASUGA010000763 DASUGA010000000
VERSION     DASUGA010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780479"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1466
                     /locus_tag="VFX21_14995"
     CDS             <1..1466
                     /locus_tag="VFX21_14995"
                     /inference="COORDINATES: protein motif:HMM:NF005863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX5097332.1"
                     /translation="AAQGVGPGDHVGIYGFNSLEFVETILAAYKLRAVPINVNYRYVE
                     AELRYLFDNADLVALVHDAQYTPRIAAVKDALPMLRHLIVIDDGSGVDASRLGSVPYD
                     EAIAAQSPERDFGPRSDDDLYILYTGGTTGMPKGVVWRQEDVIRTLGGGIDFNTGEKI
                     EDEYQFSRVAAETDEGKGIVIAPLMHGAAQWGTLNGLFNGRSTVLMPKFDAVEVWETV
                     AVHRITSIQITGDAMGRPLIEALDEIEVDVTSLVTVASTAAVFSATVKERYLEKFPDL
                     IIADAVGSTETGYNGVTVVTKGAVQKPGIPTVRMGPMTVVLDDELNVVEPGSGVVGKM
                     ARGGNVPLRYHKDPEKSAATFVTVNGMRYAVSGDNAIVEEDGSMTLLGRGSVCINSGG
                     EKIFPEEVEAALKAHPAVFDAVVVGVPDERWGQKVAAVVQLRDGHEVTLEELDAHARE
                     HVAGYKVPRELHVVDQMERSPSGKPDYPWATKVATGR"
     gene            1489..>2053
                     /locus_tag="VFX21_15000"
     CDS             1489..>2053
                     /locus_tag="VFX21_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007515122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX5097333.1"
                     /translation="MHNELCERLDIEFPIFAFSHCRDVVAAVTNAGGIGVLGALAFTP
                     EQLELECKWIDEHVGGKSYGVDVVIPASHVGSGFEDVGELLPKLQDMVPAKTLEWIDE
                     VLDELGVPPLPEDASTDAGDEGTGGLGGAGLLGWTNATSKPQVEVGLSHPVRLLANAL
                     GPPPKDVVDAAHEKGVLVAALVGRADQA"
BASE COUNT          354 a          685 c          706 g          308 t
ORIGIN      
        1 tcgcggcgca gggcgtcggg cccggcgacc acgtcgggat ctacgggttc aacagcctgg
       61 agttcgtcga gacgatcctc gccgcgtaca agctgcgcgc ggtcccgatc aacgtcaact
      121 accgctacgt cgaagccgag ctgcgctacc tcttcgacaa cgcggacctc gtcgcgctcg
      181 tgcacgacgc gcagtacacc ccgcgcatcg cggcggtgaa ggacgcgctc ccgatgctgc
      241 gccacctcat cgtcatcgac gacggcagcg gcgtcgacgc gtcgaggctc ggttcggtgc
      301 cgtacgacga ggcgatcgcc gcgcagtcgc ccgaacgcga cttcggtccc cgcagcgacg
      361 acgacctcta catcctctac accggcggca cgaccggcat gccgaagggt gtcgtgtggc
      421 gccaggagga cgtcatccgc acgctcggcg gcggcatcga cttcaatacc ggcgagaaga
      481 tcgaggacga gtatcagttc tcgcgggttg cggccgagac cgacgaaggc aagggcatcg
      541 tcatcgcgcc gctcatgcac ggcgcggcgc agtggggcac gctgaacggc ttgttcaacg
      601 ggcgttcgac ggtgctcatg ccgaagttcg acgcggtcga ggtgtgggaa accgtcgccg
      661 tccaccgcat cacgtcgatc cagatcaccg gcgacgcgat ggggcgcccg ctcatcgagg
      721 cgctcgacga gatcgaagtc gacgtgacgt cgctcgtcac ggtcgccagc accgccgcgg
      781 tgttctcggc caccgtcaag gagcgctacc tcgagaagtt ccccgacttg atcatcgccg
      841 acgcggtcgg ttcgaccgag accggctaca acggcgtcac cgtcgtcacc aagggcgcgg
      901 tgcagaagcc cggcatcccg accgtgcgga tgggaccgat gaccgtcgtg ctcgacgacg
      961 agctcaacgt cgtcgaaccg ggttccggtg tcgtgggcaa gatggcgcgt ggcggcaacg
     1021 tcccgctgcg ctaccacaag gatcccgaga agtcggcggc aacgttcgtc accgtgaacg
     1081 gcatgcgcta cgccgtgtcg ggcgacaacg cgatcgtcga agaagacggc tcgatgacgt
     1141 tgctcggtcg cggctcggtg tgcatcaact ccggcggcga gaagatcttc ccggaagaag
     1201 tcgaggccgc gttgaaggcg catccggcgg tgttcgacgc ggtcgtcgtc ggcgtccccg
     1261 acgaacgctg ggggcagaag gtcgccgccg tcgtgcagtt gcgcgacggt cacgaagtca
     1321 cgctcgaaga gctcgacgcg cacgcccgcg agcacgtcgc gggttacaag gtgccgcgcg
     1381 agctgcatgt cgtcgaccag atggaacgct caccgagcgg caagcctgac tatccctggg
     1441 ccaccaaggt cgcaaccggc cgctgacgcc caaccacacg gaggcatcgt gcacaacgaa
     1501 ctgtgcgaac ggctcgacat cgagttcccg atctttgcgt tcagtcactg ccgcgacgtc
     1561 gtcgccgcgg tcacgaacgc gggtgggatc ggcgtgttgg gcgcgctcgc attcacgccc
     1621 gagcaactcg agctcgagtg caagtggatc gacgagcacg tcggcggcaa gtcgtacggt
     1681 gtcgacgtcg tgatccccgc gtcgcacgtg gggtcgggct tcgaggacgt cggtgagctc
     1741 ttgccgaagc tccaggacat ggtcccggcc aagacgctcg agtggatcga cgaggtgctg
     1801 gacgagctcg gtgtgccgcc gctgcccgaa gacgcgtcga ccgacgccgg cgatgaaggc
     1861 accggcggtc tcggcggtgc gggtctgctc gggtggacca acgcgacatc gaagccgcag
     1921 gtcgaggtcg ggctgtcgca cccggtgcgc ctgctcgcca acgcgctcgg cccgccgccc
     1981 aaggacgtcg tcgacgccgc gcacgaaaaa ggtgtgctcg tcgccgcgtt ggtgggccgg
     2041 gccgaccagg ccc
//
LOCUS       DASUGA010000764         3004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_363674, whole genome shotgun sequence.
ACCESSION   DASUGA010000764 DASUGA010000000
VERSION     DASUGA010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363674"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VFX21_15005"
     CDS             <1..425
                     /locus_tag="VFX21_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097334.1"
                     /translation="YRCFRGKDDLLIALLADDSALGATVLAERIAARDDDGLPAFVDE
                     LFTLLTLPAADGYAGVLVREHVRLSETHTEELRVALAPLTDLLASVLTTADPQRDAQT
                     TFGVLLQGIHDVVLGHVPDARAHGAYLYRFCAHGLDGC"
     gene            425..1615
                     /locus_tag="VFX21_15010"
     CDS             425..1615
                     /locus_tag="VFX21_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5097335.1"
                     /translation="MATTTLLPDPAKRERHYTIISVDDHLIEPAHLFEGRMPAHLADK
                     APRVVTFDDGRETWVYEDRFYPQVGLNAVVGRPKDEWSMEPARFDEMRKGCWDIEARV
                     ADMDLDGVYASLCFPSLIAGFAGTVFSKSADQELGLACVRAWNDWHIDEWAAPHPDRI
                     IPLQISWLSDPEIAAADVRANAARGFKAVSFPENPVDLGLASMHTDYWDPFLRACAET
                     ETVVCLHTGSSSWTAARSPNAPLELYTSLFPVNALVTAADWLWARIPTRFPELKIAFS
                     EGGISWVPMLIDRIEYVLDHSAVGSHGWDDTSISPVEALRRNFWFCTIDIGSTIALRD
                     HIGIDHICLESDYPHADSTWPDTQASAYAGLADLPEDDVRKLTWENASKLFRHPVPAE
                     LQRP"
     gene            1612..>3004
                     /locus_tag="VFX21_15015"
     CDS             1612..>3004
                     /locus_tag="VFX21_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5097336.1"
                     /translation="MSLDLLLQGGTVVDGTGGPSRRADVGVRDGRVVAIGDVDDTATR
                     TLDVSGAVVAPGFVDLHTHYDAQLLWDPTASPSPLHGVTTLFGGNCGFALAPASPEHV
                     DYLSRLMSRVEGIPLPALQCGVPWDWKTVGDYLARVEAGGTAVNAGFLAGHSVIRRVV
                     MGDRMHEPATADDIAGMVEVLHRALDAGAMGFSSSQSHTHNDGEGEPVPSRAATREEM
                     LALAGAVADHPGTQLELIISGCLNGFSDDDIELMASMSRLAQRPLNWNVLGVSQGGMH
                     DHQLEASTRAAEQGGRVVALTLPQGMRIRLSFHTGFVLDGLPGWRETFALPVDQRMRV
                     LSDPQRRAELDERAHSPEAGVLAGLARWERLEIVEGFTERTRAYEGRKIGDIARELKQ
                     QPFDTLCDIVVEDGLRTGLRPDFGGPEPDEVWKMRAEVWRDPRAIVGGSDAGAHLDMM
                     SGASYSTFLVGDAV"
BASE COUNT          454 a         1054 c         1013 g          483 t
ORIGIN      
        1 tctaccgctg ctttcgcggc aaggatgacc tcctcatcgc gctgctcgcc gacgacagcg
       61 cgctcggcgc gaccgtgctc gccgaacgca tcgcggcgcg cgacgacgac ggccttccgg
      121 ccttcgtcga cgagctcttc acgttgctca cgctgcccgc ggccgacggc tacgcgggcg
      181 tgctcgtgcg cgagcacgtg cgcctgtcgg agacgcacac cgaagagctg cgtgttgccc
      241 tcgcgccgct caccgatctg ctcgcgtcgg tgctcacgac cgcggatccg cagcgcgacg
      301 cacaaacgac gttcggcgtg ttgctgcagg gcatccacga cgtcgtgctc ggccacgttc
      361 ccgacgcgcg cgctcatggc gcgtacctct atcgattctg tgcacacggc ttggacgggt
      421 gttgatggcg acgacgacac tgcttcccga tccggcgaag cgcgagcggc attacacgat
      481 catctcggtc gacgaccatc tgatcgagcc cgcgcatctc ttcgaagggc gcatgcccgc
      541 gcatctcgcc gacaaggcgc cgcgcgtcgt cacgttcgac gacggccgcg agacatgggt
      601 ctacgaagac cgcttctacc cgcaggtcgg cttgaacgcg gtcgtcgggc gcccgaagga
      661 cgaatggagc atggagccgg cccgcttcga cgagatgcgc aagggctgct gggacatcga
      721 ggcccgggtc gccgacatgg acctcgacgg cgtctatgcc agcctctgct tcccgtcgct
      781 gatcgccggc ttcgcgggca ccgtgttctc caagagcgcc gaccaggagc tcgggctcgc
      841 gtgcgtgcgc gcgtggaacg actggcacat cgacgagtgg gccgcgcccc accccgatcg
      901 catcatcccg ttgcagatct cgtggctcag tgatcccgag atcgcggccg ccgacgtgcg
      961 cgcgaacgcg gcgcgcggct tcaaagcggt gagcttcccc gagaatcccg tcgacctcgg
     1021 cctcgcgtcg atgcacaccg actactggga cccgttcctg cgcgcgtgcg cagagaccga
     1081 gaccgtcgtg tgcctgcaca ccgggtcgtc gtcgtggacc gcggcgcgat cgccgaacgc
     1141 gccactcgag ttgtacacga gcttgttccc ggtcaacgcg ctcgtcaccg cggccgactg
     1201 gctgtgggcg cgcatcccca cccgattccc ggagttgaag atcgcgttct ccgagggcgg
     1261 gatcagctgg gtgccgatgc tgatcgaccg catcgagtac gtgctcgacc attccgcggt
     1321 cggctcgcac ggctgggacg acacgagcat ctcgcccgtc gaggcgttgc gccgcaactt
     1381 ctggttctgc accatcgaca tcggctcgac gattgcgctg cgcgaccaca tcggcatcga
     1441 ccacatctgt ctcgagagcg actacccgca cgcggattcg acgtggcccg acacccaagc
     1501 gagcgcgtat gcggggctcg ccgaccttcc ggaggatgac gtgcgcaaac tcacgtggga
     1561 gaacgcatcg aagttgttcc gccacccggt cccggccgaa ctgcagcggc catgagtctc
     1621 gacctgctcc tccagggcgg cacggtcgtc gacggcaccg gcggtccgtc tcggcgcgcc
     1681 gacgtgggtg tgcgcgacgg acgggtcgtc gcgatcggcg acgtcgacga caccgcgacg
     1741 cgcacgctcg acgtgtccgg cgcagtcgtc gcaccgggtt tcgtcgacct gcacacgcac
     1801 tacgacgcgc aactgttgtg ggacccgacg gcgagcccgt cgcccctgca cggcgtgacg
     1861 acactcttcg gcggcaactg cggcttcgcg ctcgctcccg cgtcacctga gcacgtcgat
     1921 tacctctcgc gcttgatgtc gcgggtggag ggcatcccgt tgccggcgct gcagtgcggc
     1981 gtgccgtggg actggaagac cgtgggcgac tatctcgcgc gtgtcgaagc gggtggcacc
     2041 gcggtgaacg cgggattcct cgccgggcac tcggtcatcc gacgcgtcgt gatgggtgac
     2101 cgcatgcacg agccggcgac cgccgacgac atcgcgggga tggtcgaggt gctgcaccgc
     2161 gcgctcgacg cgggtgcgat gggtttctcg tcgtcgcagt cgcacacgca caacgacggc
     2221 gagggtgagc cggtgccgtc gcgcgccgca acccgcgagg agatgctcgc gttggccggc
     2281 gcggtcgccg accacccggg cacccaactg gaactgatca tctccggctg cctgaacgga
     2341 ttctccgacg acgacatcga gctcatggcg tcgatgtcac gcctcgcgca acgcccgttg
     2401 aactggaacg tgctcggcgt gtcacagggc ggcatgcacg accaccaact cgaggcgtcg
     2461 acccgcgccg ccgaacaggg tggacgcgtc gtggcgctga cgttgccgca gggcatgcgc
     2521 atccgcctgt cgttccacac cgggttcgtg ctcgacggtc tgccgggctg gcgcgagacc
     2581 ttcgcgctcc ccgtcgacca gcgcatgcgc gtgctctccg atccgcagcg ccgcgcggag
     2641 ctcgacgagc gcgcccactc gccggaagcc ggtgtcctcg ccgggctcgc gcgctgggaa
     2701 cggctcgaga tcgtcgaagg attcaccgag cgaacgcgtg cgtacgaggg tcgcaagatc
     2761 ggcgacatcg cgcgcgagct caaacaacag ccgttcgaca cgctgtgcga catcgtcgtg
     2821 gaagacggtt tgcgcaccgg gctgcgtccc gacttcggtg gcccggaacc ggacgaggtc
     2881 tggaagatgc gcgccgaggt gtggcgcgat ccgcgtgcga tcgtgggcgg atccgacgcg
     2941 ggcgcccacc tcgacatgat gtcgggcgcg tcgtactcga cgttcctcgt cggtgacgcg
     3001 gtgc
//
LOCUS       DASUGA010000765         2335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1753569, whole genome shotgun sequence.
ACCESSION   DASUGA010000765 DASUGA010000000
VERSION     DASUGA010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1753569"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VFX21_15020"
     CDS             complement(<1..316)
                     /locus_tag="VFX21_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX5097337.1"
                     /translation="MLRHTPRFRRAMFSGAAAAIAVVVLAGCGDSTGPDNEQNALRPA
                     GPYADKILDLTRPFFWIAVAVGVGVLAGTIYVALRFRVKPGEERMPKQTHGNTVLEVS
                     WTI"
     gene            complement(465..1361)
                     /gene="era"
                     /locus_tag="VFX21_15025"
     CDS             complement(465..1361)
                     /gene="era"
                     /locus_tag="VFX21_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX5097338.1"
                     /translation="MTEFHSGFVSLVGRPNVGKSTLLNAIVGQKVSIVSDRPQTTRTQ
                     VRGVRTTPESQIVFLDTPGVHKPRTLLGERANDRALATLAEVDAVCFVIEANAEIGRG
                     DHFVAERLAQVSTPVVLAVNKVDAATREDVLEHLASAAGELGDFAAYVPISARTGDGV
                     NALVGELDAHLPEGPHYYPEGVVSDQPESFLAAELVREQLLAVAREELPHSIAVTTDE
                     IEERETAGGPLLALRVVVRVERDSQKGMVIGKGGAVLKAAGTAARIEMEALLGTRVHL
                     DLHVKVDPDWQRRAGSLDRLGL"
     gene            complement(1444..>2335)
                     /locus_tag="VFX21_15030"
     CDS             complement(1444..>2335)
                     /locus_tag="VFX21_15030"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX5097339.1"
                     /translation="GFFPLKWLTRGFIGIANVVLPGKGLRHGPFVTEEEILTMADVAA
                     EESSIESEERELIHSIFEFGDTVVREVMLPRTDMLVVEAGATVDEAIELAISGGKSRL
                     PAFEDSTDNIVGLVFLKDLVARRSAGEGSEPVRQSLRRAHEVPESKRVAELLREMQTE
                     KFHMAIVVDEYGGTAGLVTMEDLLEEIVGEITDEYDVDEPVVERLANGALRVPGKTPI
                     DDVNELLDVDLPDEEWDTVGGLVFNSLGHVPVEGECLHVDGLEFCAERIQGRRIVSVL
                     ITRVSEPQPPEEQPAEPATS"
BASE COUNT          360 a          812 c          755 g          408 t
ORIGIN      
        1 tgatcgtcca gctgacttcc agcaccgtgt tgccgtgcgt ctgcttcggc atccgttctt
       61 cgccgggctt cacacggaag cgcaacgcga cgtagatcgt gccggcgagc acaccgacgc
      121 cgaccgcgac cgcgatccag aagaacggcc gggtcaaatc gaggatcttg tcggcgtacg
      181 ggcccgcagg tcgcaacgcg ttctgctcgt tgtccggtcc cgtgctgtca ccgcagcccg
      241 cgagcacgac gactgcgatc gcggcagcgg caccggagaa cattgcgcgc cggaaccgtg
      301 gcgtgtgcct caacatcagc ctctcttgga cagtgttctc gcgagcgaac ggtgagattc
      361 ggccggactc tacagcggtc gattctccga gccacaactc tgtcattcag aacgcgcgga
      421 gtggtggaac gcctcgaaaa ccacttgttc acaagcgctt ttggtcaaag accgagccgg
      481 tcgagcgagc ccgctcggcg ttgccaatcc gggtcgacct tgacgtgcag gtcgaggtgc
      541 acgcgcgttc cgaggagcgc ttccatttcg atccgggccg cggttcccgc ggccttgagg
      601 accgcgccgc ctttcccgat gaccatgccc ttttgcgagt cacgctcgac gcgcacgacg
      661 acacgcagcg cgagcagcgg gccacctgcg gtttcgcgct cctcgatctc gtcggtggtg
      721 acggcgatcg agtgcggcag ttcctcacgc gcgacggcaa ggagctgctc gcgcaccaat
      781 tcggccgcga ggaacgactc gggctggtcg ctgacaacac cttccgggta gtagtgcgga
      841 ccctccggga ggtgggcgtc gagctcgccg accagcgcgt tcaccccgtc tccggtgcgg
      901 gcggagatcg gcacgtatgc cgcgaagtcc ccgagctcgc cggctgcgct agccaggtgt
      961 tccagcacgt cctcgcgggt cgcggcgtca accttgttca cggccagcac caccggcgtc
     1021 gacacttgcg ccaggcgctc ggcgacgaag tggtcgcccc ggccgatctc ggcgttggcc
     1081 tcgatcacga agcagacggc gtcgacttcg gccagcgtgg cgagtgcccg gtcgtttgca
     1141 cgctcgccga gcaacgttcg gggcttgtgc acccccgggg tgtcgaggaa cacgatctgg
     1201 ctctcgggcg tcgtgcggac gccccgcacc tgggtgcggg tggtctgcgg acgatccgac
     1261 acgatcgaga ccttctgtcc gacgatcgcg ttcagcagcg tcgacttgcc gacgttggga
     1321 cgcccgacga gcgagacgaa ccccgagtga aactccgtca cactcttccg ctctcggccc
     1381 aacgggtcgg gccgctcggg ccccgctcgc tactcggcgg gatacccgcc tcccaacgct
     1441 cgctcacgag gtcgcgggct cggcgggttg ctcttccgga ggctgcggtt cggacacgcg
     1501 cgtgatcaac accgagacga ttcggcggcc ctggatgcgc tcggcgcaga actcgaggcc
     1561 gtcgacgtgc aggcactccc cctcgaccgg cacgtggccg agcgagttga acacgagccc
     1621 gccgacggtg tcccactctt cgtcggggag gtcgacgtcg agcagctcgt tgacgtcgtc
     1681 gatcggcgtc ttgcccggca cccgcaacgc cccgttcgcc agccgttcga cgaccggctc
     1741 gtcgacgtcg tactcgtcgg tgatctcgcc gacgatctct tcgagcaggt cctccatcgt
     1801 gacgagtccg gccgtgccgc cgtattcgtc gacgacgatc gccatgtgga acttctcggt
     1861 ctgcatctcg cgcagcagtt cggcgacgcg tttcgactcc ggcacctcgt gcgcccgccg
     1921 gaggctctgg cgcaccggtt cggaaccttc gccggcgcta cgccgggcga cgaggtcctt
     1981 caagaacacg aggcccacga tgttgtcggt gctgtcctcg aacgccggca ggcgcgactt
     2041 gcctccgctg atcgcgagtt cgatcgcctc gtcgacggtg gcaccggcct cgacgacgag
     2101 catgtcggtg cgcggcagca tcacctcgcg cacgacggtg tcgccgaact cgaagatcga
     2161 gtgaatgagt tcgcgttctt cgctctcgat cgacgactct tcggccgcga cgtcggccat
     2221 cgtcaggatc tcttcttcgg tcacgaacgg cccgtggcgc aaacccttgc cgggcagcac
     2281 gacgttggcg atgccgatga agccgcgcgt cagccacttc agcgggaaga acccc
//
LOCUS       DASUGA010000766         2427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1197665, whole genome shotgun sequence.
ACCESSION   DASUGA010000766 DASUGA010000000
VERSION     DASUGA010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197665"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VFX21_15035"
     CDS             <1..223
                     /locus_tag="VFX21_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015746716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5097340.1"
                     /translation="KEALESLPEQFRMAVILADIEGFSYKEIAEILDVPIGTVMSRIH
                     RGRKQLQKRLWDYAERNNLRPHAETSASG"
     gene            240..476
                     /gene="rsrA"
                     /locus_tag="VFX21_15040"
     CDS             240..476
                     /gene="rsrA"
                     /locus_tag="VFX21_15040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HEX5097341.1"
                     /translation="MDCDHAIHRVYEYLDGELTVWKRRAIARHLDECPPCAEGFVFEL
                     EIRQVIVSKCQERAPDELRARIAKALGFEGDLPV"
     gene            559..786
                     /locus_tag="VFX21_15045"
     CDS             559..786
                     /locus_tag="VFX21_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097342.1"
                     /translation="MKPTTIMSLLSAVLIAVGVIFLGVYFFADREGGKEWFYWAAPLL
                     LIAFGGMLGSLVRQYWVRVGKLETKGRPRSA"
     gene            797..1876
                     /locus_tag="VFX21_15050"
     CDS             797..1876
                     /locus_tag="VFX21_15050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX5097343.1"
                     /translation="MAPAPAPPVPEPVDWATARKVARIVAGRDPLADSYLGSSLDTDF
                     AFLTPHAEDLVADFTGLRPPGRAHASVLDRRGWVDANVASMQHLLKPLMERMAPRVAR
                     SPIAPVGRQIAGTEVGALVGYLAQRVLGQYDMLVPDDQSGDAVYYVGPNILALEKRFA
                     FRPQDFRLWIAIHEVTHRAQFTGVRWLREYFFGLVQQAFQVVDPDPRSFVGALARAVD
                     EVRAGRNPLDDGGMVGLFATPEQRTVLQQVQALMSLVEGHGNFVMDELGREHVAGAER
                     MQRVLHARREVRGVAGQVHKLLGIEQKLKQYDIGERFVRGVVDEAGLAAVDNAWRSPA
                     HLPTHAELDDAPSWLRRVETIDVAG"
     gene            1863..>2427
                     /locus_tag="VFX21_15055"
     CDS             1863..>2427
                     /locus_tag="VFX21_15055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5097344.1"
                     /translation="MSPADGPLLLVVDDEPDIRELVRVNLEAEGYRVVTANDGDQALS
                     VARDNPPDAVFLDVLMPGRDGWSVLRELKSGDPNLEAVPVIMVTALSQTEDRLRGAIE
                     GAVRYLTKPFHPRELLTVLDELLAPDAPPESELRRRARQDALETLARIESGNEHAPSG
                     GVPRVRLTRLEHGPVREMLPARVSETRD"
BASE COUNT          380 a          807 c          838 g          402 t
ORIGIN      
        1 gaaggaagcg ctcgagtcgc tgccggagca gttccggatg gcggtgatcc tggccgacat
       61 cgaagggttc tcctacaagg agatcgccga gatcctcgac gtcccgatcg ggaccgtgat
      121 gagccggatc caccgcggaa gaaagcagtt gcagaagcgg ttgtgggatt acgccgagcg
      181 caacaatctc cgaccgcacg ccgagacatc ggcgtcgggc taggcgaggc acaggcgaga
      241 tggactgcga ccacgccata caccgcgtct acgagtatct cgacggtgag ctgaccgtct
      301 ggaagcgacg ggcgatcgcc cggcatctcg acgagtgccc gccgtgcgcc gagggcttcg
      361 tgttcgagct cgagatccgg caggtgatcg tctccaagtg ccaggagaga gcgcccgacg
      421 agctgcgcgc ccgcatcgcc aaggcgttgg gcttcgaagg cgacctcccg gtctgagccc
      481 gcccccgagt cacggccggc ggagcggccc gacctacgat ggccggcgat cgctggccga
      541 gcgttgttgg aggatctggt gaagcccacg accatcatgt cgctgctgtc ggcggtgttg
      601 atcgccgttg gggtcatctt cctcggcgtc tacttctttg ccgaccggga aggcggcaag
      661 gagtggttct actgggccgc gccgctgttg ctcatcgcgt tcggcggcat gctcgggtcg
      721 ctcgtacggc agtactgggt gcgcgtcggc aagctcgaga cgaagggccg cccgcgcagt
      781 gcctgatgcg gccgccgtgg cgcccgcacc ggcgccgccc gtgccggagc ccgtcgactg
      841 ggcgaccgcg cgcaaggtcg ctcgcatcgt cgccggtcgc gacccgctcg ccgactcgta
      901 cctcggctcc tcgctcgaca ccgacttcgc gttcctgacg ccgcacgccg aggacctcgt
      961 cgccgacttc acgggattgc gtccgcccgg ccgcgcgcac gcgagtgtgc tcgaccgccg
     1021 cggctgggtc gacgcgaacg tcgcgtcgat gcagcacctg ctcaagccgt tgatggagcg
     1081 catggcgccc cgcgtcgcgc gtagcccgat cgcaccagtc gggcgccaga tcgcgggcac
     1141 cgaggtcggc gcactcgtcg gctatctcgc acaacgcgtg ctgggtcagt acgacatgct
     1201 cgtgcccgac gaccaatcgg gtgacgccgt gtactacgtc ggcccgaaca tcctcgccct
     1261 ggagaagcgg ttcgctttcc ggccgcagga tttccgattg tggatcgcta tccatgaagt
     1321 gacccatcgc gcgcaattca ccggagtccg gtggttgcgc gaatactttt tcggcctggt
     1381 gcaacaagca ttccaagtcg tagaccccga tccgcgctcg ttcgtcggcg cgctggcacg
     1441 tgcagttgac gaagtgcgcg cgggccgcaa tcctctggac gacggcggaa tggtcgggtt
     1501 gttcgccacg cccgagcaac gcaccgtctt gcagcaagtg caagctctga tgtcgttggt
     1561 cgaagggcac gggaacttcg tcatggacga actcggccgt gaacatgtcg cgggcgcgga
     1621 gcgcatgcaa cgcgtgttgc acgcgcgtcg cgaagttcgt ggtgtcgccg gtcaggtcca
     1681 caagttgttg ggtatcgaac agaagttgaa gcagtacgac atcggtgagc gattcgtgcg
     1741 cggtgtcgtc gacgaagccg ggctcgcggc cgtcgacaac gcgtggcgtt cgccggcgca
     1801 cctgccgacg catgccgagc tcgacgacgc cccgagttgg ttgcggcgcg tggagacgat
     1861 cgatgtcgcc ggctgacggg ccgctcctgc tcgtcgtcga cgacgagccc gacatccgcg
     1921 agctcgtgcg cgtgaacctc gaagccgagg gctaccgcgt ggtcaccgcc aacgacggcg
     1981 atcaggcgtt gtcggtcgcg cgtgacaatc cgcccgacgc cgtcttcctc gatgtgctca
     2041 tgccgggccg cgacggttgg tcggtgttgc gcgagctgaa gtccggtgac ccgaatctcg
     2101 aggccgtgcc ggtgatcatg gtcaccgcgc tgtcacagac cgaggaccgc ctgcggggcg
     2161 cgatcgaggg cgcggttcgg tacctcacga agcccttcca cccacgcgaa ctgctcactg
     2221 tgctcgacga actgctcgcg ccggatgcgc ccccggagtc cgagctgcgg cgccgcgccc
     2281 ggcaggacgc cttggagaca ctcgcccgta tcgagagcgg caacgagcac gcgccgtccg
     2341 gaggggtgcc gcgcgtccgg ttgacccggc tcgagcacgg cccggtgcgc gagatgttgc
     2401 ccgcgcgcgt gagcgagacg cgtgacc
//
LOCUS       DASUGA010000767         3756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1891802, whole genome shotgun sequence.
ACCESSION   DASUGA010000767 DASUGA010000000
VERSION     DASUGA010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891802"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1195)
                     /locus_tag="VFX21_15060"
     CDS             complement(41..1195)
                     /locus_tag="VFX21_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5097345.1"
                     /translation="MTHTDPEVRAEMVATVRRWIEREVAPIASEHEHADAYPEAIVEQ
                     MKGLGLFGITIPEQYGGLGLDLLTYIGVVEELAYGWMSLSGIVNTHTIAAYLIVNHGT
                     EAQKQRWLPRLASGELRGCLSLSEADAGSDTRNISCKATRDGDEYIVNGTKFWVTNGE
                     RAGIVALAARTEEGISCFIVEKEPGPTSGGLTVSKNIPKLGYKGIETVEMSYVDHRVP
                     ADALLGDLGRGLGYILGALEVGRINIAARAVGVARAAFDASLQYAQERQTFGKPIAQH
                     QAIQMKLADMATKLEAARLMARNAAERKQSGERADVEAGMAKLFASETAFELASEAMR
                     IHGGVGYTTELPVERYYRDAPLMIIGEGTNEIQRLVIARGLLARADAAKG"
     gene            1327..3195
                     /locus_tag="VFX21_15065"
     CDS             1327..3195
                     /locus_tag="VFX21_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5097346.1"
                     /translation="MMDHRTAYGFTQRDALKKRRVDRATMRRVLTFIRPYRAQLVWFV
                     LAVIGGAIAVAIPPLLFRSLLDTAVPDKNRTLVGLLAAAAVGLAFANALLSLVQRFYS
                     ARIGEGLIYDLRVSLFDHVQRMPIAFFTRAQTGALQSRLNSDIVGAQQAVTSTLGTVV
                     SNVITLVVTLSIMLRLEWRLTILTLLVLPAFLWPARRMGPRLQALTREGMQLNAEMNN
                     LTAERFNVAGALVAKLFGRPKHERDEFATRARGVRDIGIRSAMYSRVLFVALGLVAAV
                     GSAVVYWVGGNLAVSGALSVGTVAAFVIYVGQIYQPLVQLTNSRVEVLTALVSFERVF
                     EVLDFAPTIVDKDDAAELEASRGQVAFDHVWFRHPPGIDVSLASLEAPGTYVSEEPSD
                     WILRDVSFIAAPGETIALVGPSGAGKTTIAMLVPRVYDVSQGAVRIDGADVRDVTLDS
                     LHETVGLVAQDPHLFHDTIRNNLRYARPGATDEELRNALEAARILDLVDSLPDGLETV
                     VGERGYRMSGGEKQRLAIARVLLKDPAVVILDEATSHLDSESEVAIQNALDDALRGRT
                     AIVIAHRLSTIVGADRILVVDGGRIVESGTHAQLLAGRGIYADLYTTQLSSQAESA"
     gene            3273..>3756
                     /locus_tag="VFX21_15070"
     CDS             3273..>3756
                     /locus_tag="VFX21_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097347.1"
                     /translation="MPDDDVSPTSRSDRLARRRHRIRWVAVLVVALIVLAAGSVAAFT
                     LTDEQPAVTSATTVPAGRVDNTSGTEHSLGDVSDVSSTAPTRPLSNDEPLRLWIGGDS
                     LSGSLGMALGEMTAPLGIVKATVDYKISSGLSNKIRDWPDYAESAMKKNDPEVVVFMV
                     G"
BASE COUNT          571 a         1314 c         1233 g          638 t
ORIGIN      
        1 ggcttttgcg gagcacgggc aaaatctact cgcgctcgga ctatcccttg gccgcgtccg
       61 cgcgcgcgag caggccccgg gcgatcacca gacgctggat ctcgttggtc ccctcgccga
      121 tgatcatgag cggcgcatcc cggtagtagc gctcgaccgg cagttcggtc gtgtaaccga
      181 caccgccgtg gatgcgcatc gcctcgctcg ccaactcgaa cgcggtctcg ctcgcgaaca
      241 gcttcgccat gcccgcctcg acgtcggcgc gctcaccgga ctgcttgcgt tcggcagcgt
      301 tgcgggccat cagacgggcc gcttcgagct tcgtcgccat gtcggcgagc ttcatctgaa
      361 tcgcctggtg ctgcgcgatc ggcttcccga atgtctggcg ctcctgcgcg tactgcaacg
      421 acgcgtcgaa cgcggcgcgg gcgacaccga cggcgcgcgc cgcgatgttg atgcgcccga
      481 cctcgagcgc gccgaggatg tagccgaggc cgcgaccgag atcgccgagc aacgcgtccg
      541 ccgggacccg atggtcgaca tacgacatct cgaccgtctc gatgcccttg taaccgagct
      601 tcgggatgtt cttgctgacg gtcagaccgc cactcgtcgg gccgggttcc ttttcgacga
      661 tgaagcagga gatcccttct tcggtacgcg ccgcgagcgc gacgatgccg gcgcgttcgc
      721 cgttggtgac ccagaacttc gtgccgttga cgatgtactc gtcaccgtcg cgcgttgcct
      781 tgcacgagat attgcgggtg tcgctgccgg catcggcctc cgacagcgag agacaaccgc
      841 gcagctcacc ggacgcgagt cgcggcagcc atcgttgctt ctgcgcctcg gtgccgtggt
      901 tgacgatcag gtacgcggcg atggtgtggg tgttgacgat gccggacagc gacatccagc
      961 cgtacgcgag ctcttcgacg acaccgatgt aggtcagcag gtcgagaccg agcccgccgt
     1021 actgctcggg aatcgtgatc ccgaagaggc cgaggccctt catctgctcg acgatcgctt
     1081 cggggtacgc gtcggcgtgc tcgtgctcgg acgcgatcgg cgccacttcg cgctcgatcc
     1141 accgccggac ggtcgcgacc atctcggcgc gcacttcggg atctgtatgt gtcacgaggc
     1201 ggatgctaga gagacgcgcc ggtcagggca tcagccagct ctgcccggcc ccgcaccccg
     1261 agcttctcgt aggcgcgggt gtcaggggac cgggcctcgg ggacgggccg gtaatgtgcg
     1321 gcgcgcatga tggatcaccg caccgcgtac ggcttcaccc aacgagacgc attgaagaag
     1381 cggcgtgtcg accgcgccac gatgcggcgg gtcctgacct tcattcgccc ctaccgagcg
     1441 caattggtgt ggttcgtgct ggccgtgatc ggcggcgcga tcgccgtcgc gatcccgccg
     1501 ctgttgttcc gcagcctcct cgacaccgcg gtccccgaca agaaccgcac cctcgtcggc
     1561 ctgctcgcgg ccgccgcggt cgggctcgcg ttcgcgaacg cgctgctctc gctggtgcag
     1621 cgcttctact cggctcgcat cggcgagggg ctcatctacg acctgcgcgt ctcgttgttc
     1681 gaccacgtgc aacgtatgcc gatcgcgttc ttcacacgag cgcagaccgg agcgctgcag
     1741 tcgcggttga acagcgacat cgtcggcgcg caacaagccg tgacgtcgac gctcgggacc
     1801 gtcgtctcca acgtgatcac cctcgtcgtg acgctgtcga tcatgttgcg gctcgaatgg
     1861 cggctgacga tcctgacgct gctcgtgctg cccgcgttcc tctggcccgc gcggcgcatg
     1921 ggcccccgcc tacaggcgtt gacgcgcgag ggcatgcagc ttaatgccga gatgaacaac
     1981 ctgacggcgg aacgcttcaa cgtggccggc gcgctcgtcg cgaagttgtt cggccgcccg
     2041 aagcacgaac gtgacgagtt cgcaacgcgg gcgcgtggcg tacgcgacat cggcattcga
     2101 tcggcgatgt acagccgtgt gctgttcgtc gcgctcggtc tcgtcgccgc cgtcgggagc
     2161 gcggtcgtgt actgggtcgg cggcaacctc gcggtgtcgg gtgcgctgtc ggtgggcacc
     2221 gtcgccgcgt tcgtgatcta cgtcggccag atctaccaac ccctcgtgca gctcacgaac
     2281 tcccgcgtcg aggtgttgac cgcgctcgtc tcgttcgagc gcgtgttcga ggtcctcgac
     2341 ttcgcaccca cgatcgtcga caaggacgac gcggcggagc tcgaggcctc ccgcgggcaa
     2401 gtcgcattcg accacgtctg gttccggcat ccacccggca tcgacgtctc gctcgcgtcg
     2461 ttggaggccc cgggcaccta cgtctccgag gagccgagcg actggatcct gcgcgacgtc
     2521 tcgttcatcg ccgcgccggg cgagacgatc gcgctcgtcg gaccgtcggg cgcgggcaag
     2581 acgacgatcg cgatgctcgt gccacgtgtc tacgacgtct cgcagggcgc agtccgcatc
     2641 gacggcgccg acgtgcgcga cgtcaccctc gattccctgc acgagaccgt cggcctggtc
     2701 gcgcaggacc cgcatctgtt ccacgacacg atccgcaaca acctgcgcta cgcccgtccc
     2761 ggcgcgaccg acgaagagct gcgcaacgca ctcgaggcgg cgcgcatcct cgacctcgtc
     2821 gactcgctcc ccgacggtct cgagaccgtg gtcggcgaac gcgggtaccg catgtccggc
     2881 ggcgagaagc agcggctcgc gatcgcgcgc gtgctgctga aggatccggc ggtcgtcatc
     2941 ctcgacgaag cgacgtcgca tctcgactcc gagtcggagg tcgcgatcca gaacgcgctg
     3001 gacgacgcgt tgcgcggccg caccgccatc gtcatcgcgc accgcctgtc gacgatcgtc
     3061 ggcgccgacc gcatcctcgt cgtcgacggc ggacggatcg tcgaatccgg cacacacgca
     3121 caattgctcg ccggccgcgg catatacgcc gacctctata cgacgcagct gagctcacag
     3181 gccgaatccg cctgaccgcg gcgcggcggt agcatcattt gattcacgct gcggcccggt
     3241 aggtaggtcg cgaccaacca cctcccgcac cgatgcccga cgacgacgtc tccccgacca
     3301 gccgcagcga ccggctcgcg cgccggcggc accgcatccg ctgggttgcg gtgctcgtcg
     3361 tcgcgttgat cgtgctcgcg gccggcagcg tcgccgcgtt caccctcacc gacgagcagc
     3421 ccgcggtcac gtccgcgacg acggtgccgg cgggtcgggt cgacaacacg tcgggtaccg
     3481 aacattccct gggcgacgtg agcgatgtct cgagcaccgc cccgacgcgc ccactctcga
     3541 acgacgaacc gttgcggctg tggatcggcg gcgattcgct gtcgggctcg ctcggtatgg
     3601 cgctcggcga gatgaccgcg ccattgggca tcgtgaaggc gacggtcgac tacaagatct
     3661 cgagcgggct cagcaacaag atccgcgact ggcccgacta cgccgagagc gcgatgaaga
     3721 agaacgaccc cgaggtcgtg gtcttcatgg tcggcg
//
LOCUS       DASUGA010000768         4892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1791325, whole genome shotgun sequence.
ACCESSION   DASUGA010000768 DASUGA010000000
VERSION     DASUGA010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791325"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VFX21_15075"
     CDS             complement(<1..249)
                     /locus_tag="VFX21_15075"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEX5097348.1"
                     /translation="MAGDSSQRTEKPTPKRLREAREKGQIARSPDLSAWAGMLVAVFL
                     VQMTGSIAARVFPEILYNMGQVMARPDAERAWKFTGDSL"
     gene            complement(253..1002)
                     /locus_tag="VFX21_15080"
     CDS             complement(253..1002)
                     /locus_tag="VFX21_15080"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEX5097349.1"
                     /translation="MEVAFDPELVAAFVLALVRASTWLFIAPPFNSRLMPVSIKIGVA
                     AALALAAAPHIANPDLSLETGPFVGALVTQALVGFTLGMLTMLLVGAIQSAGALIDIF
                     AGYSMVMLYDPFSDSSTSMFGRFYGLIATTLLFTTNAHLILINGFFRSFEAVPLGGLP
                     SGDIARLLTDNLGQFGIAALEVAGPVLACLFLTEITLGLLNRAAPSLNVFSLAFPLRV
                     VVALIVVAIAIPLIAPALTNLVTQAVTPFGG"
     gene            complement(1007..1279)
                     /locus_tag="VFX21_15085"
     CDS             complement(1007..1279)
                     /locus_tag="VFX21_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HEX5097350.1"
                     /translation="MSDSSVIDIAVQSMILATKLAGPILIVSLAIGLGIGLLQSATQV
                     QEQTLAFVPKLAGVALVIVLAGHWMLSQFIGFTVNLFDMIPTLISG"
     gene            complement(1309..2196)
                     /gene="fliP"
                     /locus_tag="VFX21_15090"
     CDS             complement(1309..2196)
                     /gene="fliP"
                     /locus_tag="VFX21_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076251.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEX5097351.1"
                     /translation="MPASERAAKVKKLRRSLVIFLVIFAAIGVETVVAQYAFAQTVET
                     PETPRAPQTPTPGGNETTETTTPDDASISINLPDVGTTEDGKEKPGQSVVIILVLTLL
                     AIAPALLMMLTSFTRIVIVLSLTRNALGLTAIPPNQVVIGLSLFLSLFIMSPVLKEVN
                     ETALQPLLDGKMSYSEAYDAAVIPIKGFMLDQTRSSELNLFIKASGEPAPKDRDDISM
                     ATLVPAFVLSELKTAFIIGFVVFVPFLIIDLVVSSALMSMGMFMLPPVLVSLPFKLLL
                     FVMVDGWALVVQSLITSFH"
     gene            complement(2227..2646)
                     /locus_tag="VFX21_15095"
     CDS             complement(2227..2646)
                     /locus_tag="VFX21_15095"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEX5097352.1"
                     /translation="MSTTSTFALFARLIFSMGIVLGLMWIAARVLQRRGLGPVAKRQA
                     RAVELELLARKNVGRHASIAVVRAAGQAMVVGITEHEITKLADADLPAIDLEAEQDVA
                     ASEAASQGTAAPQQGFATPGQAWKAMLEHVREKTVRR"
     gene            complement(2646..3458)
                     /gene="fliN"
                     /locus_tag="VFX21_15100"
     CDS             complement(2646..3458)
                     /gene="fliN"
                     /locus_tag="VFX21_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEX5097353.1"
                     /translation="MTLISDLATTNDVAMTVAAAIERVLGDDLILAVGLPRMEPPTSD
                     VLPSGPLHAVRAPFFGDADGSIMVFVGDGLATVLTTTSPDESLAGALAGAVQAGAEEV
                     VRVSDGRVSAGDPMEINASEVLSHPLDGEFLVYPLLDGNERIACIVVQIADANGATAA
                     PGAPQTAVAVHEFQPLEGESLGYLDPRPLSLLSDVEMGVTAELGRRRMTVRELLGLTP
                     GSVIELDRAAGTPVDVLVNGTLIARGEVVVIDEEFGIRVSEIVTAELGTGSR"
     gene            complement(3464..4366)
                     /gene="fliM"
                     /locus_tag="VFX21_15105"
     CDS             complement(3464..4366)
                     /gene="fliM"
                     /locus_tag="VFX21_15105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEX5097354.1"
                     /translation="MTAVGARKGNVVPFDFRRQSKFSRDHVRALQIVHETFARQLSTV
                     LATTLRATASCTFSKIEQLTYDEYVRSLPNPSYMVILSLNPLPGAAVLQLPLPITFAA
                     IDRLLGGTGDALSPKRQLTEIESNLMRGVVDRALRELEYAYESLVRVEAEIVSQEFNP
                     QFAQVAAPSDMALVIAFETRIGDKRGNASICIPFSTMQPVIESLASQSLFNDTRGHDP
                     EVWQSELEHALTSVPVELRVRFDSVALTSTEIIGLEVGDVVPLGHAVDQPLTVTVDGV
                     RCYRAVTGRRGKRLACLIIGPDEE"
     gene            complement(4659..>4892)
                     /locus_tag="VFX21_15110"
     CDS             complement(4659..>4892)
                     /locus_tag="VFX21_15110"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEX5097355.1"
                     /translation="VGVTLELAHGSDTEAQETHGTGGLARSELIAALSGKSMDELYNA
                     ETKDQILHQLGYSVCKKTEGGVVKAYYTDFVMQ"
BASE COUNT          850 a         1629 c         1656 g          757 t
ORIGIN      
        1 gagtgagtca ccggtgaact tccaggcgcg ctccgcgtcg ggccgcgcca tgacctggcc
       61 catgttgtag aggatctcgg ggaagacgcg cgccgcgatg gaaccggtca tctggaccag
      121 gaacaccgcc acgagcattc cggcccaggc actgaggtcc ggtgaccgcg cgatctgacc
      181 cttctcacgg gcctcgcgca gccgcttcgg ggtcggcttc tcggtgcgtt gcgacgaatc
      241 gccggccatc ggctagccgc cgaacggtgt gacggcctgc gtcaccaggt tggtgagggc
      301 cggcgcgatg agcgggatcg cgatcgcgac cacgatcaac gcgacgacga cgcggagcgg
      361 gaacgcgagc gagaacacgt tgagactcgg cgcggcgcgg ttgaggagcc cgagcgtgat
      421 ctccgtgagg aacaggcagg ccagcaccgg gcccgcgact tcgagcgcgg cgataccgaa
      481 ctgcccgagg ttgtcggtca gcaagcgtgc gatgtcaccg gacggcagac cgccgagcgg
      541 caccgcctcg aacgaccgga agaacccgtt gatcaagatg aggtgcgcgt tcgtcgtgaa
      601 gagcaacgtc gtggcgatca ggccgtagaa gcggccgaac atcgacgtcg agctgtcgct
      661 gaacgggtcg tacagcatca ccatcgagta gcccgcgaag atgtcgatga gcgcgccggc
      721 cgactggatc gcgccgacga gcaacatcgt cagcatcccg agggtgaacc cgacgagcgc
      781 ctgggtgacg agcgcgccga cgaacgggcc cgtctcgagt gagaggtcgg gattggcgat
      841 gtgcggtgcg gcggccagcg cgagcgcggc cgcgaccccg atcttgatcg acaccggcat
      901 cagccgcgag ttgaacggcg gcgcgatgaa caaccacgtc gacgcccgca ccagcgcgag
      961 cacgaacgcg gcgaccagtt cggggtcgaa cgcgacctcc acgtacttat ccggagatga
     1021 gcgtggggat catgtcgaag aggttcaccg tgaacccgat gaactgcgac agcatccagt
     1081 gaccggccag cacgatgacg agcgcgacgc ccgcgagctt cggaacgaac gcgagcgtct
     1141 gctcctggac ctgcgtcgcc gactgcagca agccgatacc gagaccgatc gcgagtgaca
     1201 cgatcaggat cggccccgcg agcttcgttg cgaggatcat tgactgtacg gcgatgtcga
     1261 tgaccgaact gtcagacatg ggttctccgc ccctacgagt ttgcgtagct aatggaacga
     1321 cgtgatgagt gactgcacga cgagcgccca gccgtcgacc atcacgaaca acaacaactt
     1381 gaacggcagc gacacgagca ccggtgggag catgaacatg cccatcgaca tcagcgccga
     1441 actgacgacc aagtcgatga tcaagaacgg cacgaacacc acgaagccga tgatgaacgc
     1501 cgtcttcaac tccgacagca cgaaggcggg caccagcgtc gccatgctga tgtcgtctcg
     1561 gtccttcggc gccggctcac cgctggcctt gatgaagagg ttcagctcgc tcgagcgggt
     1621 ctggtcgagc atgaacccct tgatcgggat cacggccgcg tcgtaggcct cgctgtacga
     1681 catcttcccg tccagcagcg gttggagcgc ggtctcgttc acctctttca acaccggcga
     1741 catgatgaag agactcaaga agagcgacag cccgatcacg acctggttgg gcgggatcgc
     1801 cgtgagaccg agtgcgttgc gcgtcagcga gagcacgatg acgatgcgcg taaagctcgt
     1861 cagcatcatc aacagcgcgg gcgcgatggc cagcagcgtc agcacgagga tgatcacgac
     1921 gctctgcccg ggcttctcct tgccgtcctc ggtcgtgccg acatccggca ggttgatgct
     1981 gatcgacgca tcgtcgggcg tcgtcgtctc ggtcgtttcg ttgccgcccg gcgtcggtgt
     2041 ctggggcgcg cgcggtgtct ccggcgtctc gacggtctgc gcgaaggcgt actgcgcgac
     2101 cacggtctcg acaccgatgg cggcgaagat caccaagaag atgacgagcg accgccgcag
     2161 cttcttgacc ttggcggctc tctcacttgc aggcacggag cgctcccgga gaggctcaag
     2221 tgatgcctag cgccggacgg tcttctcccg tacgtgctcc agcatcgcct tccatgcctg
     2281 cccgggggtc gcgaatccct gctgcggtgc tgccgtccct tggctcgccg cttccgatgc
     2341 tgcaacgtct tgttccgctt cgagatcgat ggccggcaga tccgcgtcgg ccagtttggt
     2401 gatctcgtgt tcggtgatgc cgacgaccat tgcctggccg gccgcgcgca ccaccgcaat
     2461 cgacgcgtgc cgcccgacgt tcttgcgggc gaggagctcg agctcgaccg cgcgcgcttg
     2521 gcgcttcgcg acggggccga ggccccttcg ctggagcacg cgcgcggcga tccacatcag
     2581 ccccagcacg atgcccatcg agaagatgag ccgcgcaaag agcgcgaacg tcgacgtcgt
     2641 cgacatcagc gcgagccagt accgagttcg gccgtgacga tctccgacac gcgaatgccg
     2701 aactcctcgt cgatcacgac cacttcgccg cgcgcgatga gcgtgccgtt cacgagcacg
     2761 tcgacgggcg ttccggccgc acgatccaac tcgatgacgg agccgggcgt gagcccgagg
     2821 agctcgcgga ccgtcatacg gcgccggccg agctcggcgg tcacacccat ctccacgtcg
     2881 gagagcagcg acagcggccg cgggtcgagg tagccgaggc tctcgccctc gagcggctgg
     2941 aactcgtgca ccgcgactgc ggtctgcggg gcgccgggcg cggccgtcgc gccgttcgcg
     3001 tcggcgatct gcacgacgat gcacgcgatg cgctcgttgc cgtcgagcag cgggtagacg
     3061 aggaactcgc cgtcgagcgg gtgcgacagc acctccgacg cgttgatctc catcgggtcg
     3121 ccggcgctga cgcggccgtc cgacacgcgg acgacctctt cggcgcctgc ttgcaccgcg
     3181 ccggccagcg cgccggccag tgactcgtcg ggcgacgtcg tcgtgagcac ggtcgcgagc
     3241 ccgtcgccga cgaacaccat gatgctgccg tcggcgtcgc cgaagaaggg cgcccgcacc
     3301 gcatgcagcg gaccggacgg caacacgtcg gaggtcggcg gctccatgcg cgggaggcca
     3361 accgcgagga tgaggtcgtc gccgaggacg cgttcgatcg cggcggcgac ggtcatggcg
     3421 acgtcgttcg tcgtcgcgag atccgagatg agtgtcatgc cccctactcc tcgtctggac
     3481 cgatgatcag acaggccagg cgcttgccgc gccggccggt cacggcccgg tagcaccgca
     3541 caccgtcgac ggtgacggtc aacggctggt cgaccgcgtg cccgagcggc accacgtcgc
     3601 cgacctcgag cccgatgatc tcggtcgacg tcagcgcgac actgtcgaag cgcacgcgca
     3661 gctcgaccgg tacggacgtc aacgcgtgtt cgagctccga ctgccatacc tcggggtcgt
     3721 gcccacgcgt gtcgttgaac agcgactggc tcgcgaggct ctcgatgacc ggctgcatcg
     3781 tcgagaacgg gatgcagatg ctcgcgttcc cgcgcttgtc gccgatgcgc gtctcgaacg
     3841 cgatgacgag cgccatgtcg gatggcgcgg caacctgcgc gaactgcggg ttgaactcct
     3901 gcgacacgat ctcggcttcg acgcgcacga gtgactcgta cgcgtactcg agctcgcgca
     3961 gggcgcggtc cacgacgccg cgcatcaagt tcgactcgat ctcggtgagt tgccgcttcg
     4021 ggctcagcgc gtcgccggtc ccgccgagca gccggtcgat cgcggcgaag gtgatcggca
     4081 gcggcagttg gagcaccgcg gcaccgggca gcgggttcag cgacaggatc accatgtacg
     4141 acggattcgg gagtgaccgc acgtattcgt cgtaggtgag ctgctcgatc ttgctgaacg
     4201 tgcacgacgc ggtggcgcgc agggtcgtgg cgagcacagt ggagagctgg cgcgcgaacg
     4261 tctcgtggac gatctgcaac gcgcgtacgt ggtcgcggct gaacttgctc tggcgccgga
     4321 agtcgaacgg gacgacgttc cccttgcgag cacctaccgc ggtcacgcct tcacatcggc
     4381 ccgcgctccg cgcaggtaag gcaatcagcg gcgccgaagc gcccttgagg ccttctcggc
     4441 cactctcgga gtcggagggc gtggacaagg ccgcgatctt cggtcctccg tcggcgcgca
     4501 gcgcccctcg aggaccggcg cgagacgccg cacgaggcgg tggcctcaat atccccgatg
     4561 gcgcgaagcg ccccctcgat cctctcggcc actcgcggag cggaggcggc gacaagcacc
     4621 gaagccgcac acgcgcgcgc cgccggagcg acccagagtt actgcattac gaagtcggtg
     4681 tagtacgcct tgaccacgcc gccctcggtc ttcttgcaga ccgagtagcc gagctggtgg
     4741 aggatctggt ccttggtctc ggcgttgtag agctcgtcca tcgacttgcc gctcagcgcg
     4801 gcgatcagct cgctgcgagc cagcccgccg gtgccgtggg tctcctgcgc ctcggtgtcg
     4861 gagccgtgcg ccagctccag ggtgaccccg ac
//
LOCUS       DASUGA010000769         2222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_86623, whole genome shotgun sequence.
ACCESSION   DASUGA010000769 DASUGA010000000
VERSION     DASUGA010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86623"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1071)
                     /locus_tag="VFX21_15115"
     CDS             complement(40..1071)
                     /locus_tag="VFX21_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX5097356.1"
                     /translation="MLTIASDVHRVHHPHQPIHDGGRLVPPPEIPERAERIRAAIDEA
                     ALGPVREPDEFGPEPLLRVHTTDYVEFLATAFTDWQAATGELDDAEAVPYARPVRDQP
                     HNDAHHIIARLGWYSHDSDPILAGTWTAATGAVDVALTAWRAVAEEGAAHAYALCRPP
                     GHHAAADGFAGYCYLNNAAIVAQAWVDAGARVALLDVDFHHGNGTQQIFYARDDVLFT
                     SLHADPAEDYPYFLGFSEEKGWGAGEGFTRNFPLPAGTGGDAYLAAFADANAAITHFA
                     PDALVVSLGVDTALEDPDSFRLVADDFTGIGRAIGALGLPTVVVQEGGYSLDVIGRNV
                     VNVLRAIES"
     gene            complement(1084..1449)
                     /locus_tag="VFX21_15120"
     CDS             complement(1084..1449)
                     /locus_tag="VFX21_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097357.1"
                     /translation="MGRRQRDDALGWLRLQGRTNARLHLLARDLGARFVAPLDREAFG
                     AVRGRLAGCDFDLHVVPDTSDTALTGVVRHSLELALARENTNARRRFSGDGLVPPNVD
                     PARARSALEAFCFSFVSRG"
     gene            1503..2177
                     /locus_tag="VFX21_15125"
     CDS             1503..2177
                     /locus_tag="VFX21_15125"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEX5097358.1"
                     /translation="MPSVQVWRVDLDQPPPAVTALATVLDEDERAAAAKRVGSVRDRY
                     VVAHGALRTVLAHVAGGAAGALVLDRTCGHCGDPRHGRPTLAGSDVEFNLSHSDGIAL
                     IACTHAVRVGVDVEVVRPRSLLPKLARRVLDEESYEAWLARPEAEQLGVFIQEWTARE
                     AYLKGVGLGIVRRLRDVPASPPGWTITALPVGELARASLAVEHPDPPGVRLATWSLAG
                     TGTLER"
BASE COUNT          331 a          758 c          819 g          314 t
ORIGIN      
        1 caagggcgga atccgccctt gtaagacgcc cgaccgtcgt taggactcga tggcgcggag
       61 gacgttgacg acattgcggc cgatgacgtc cagggagtag ccgccttcct gcaccacgac
      121 ggtcggcagg cccaacgcgc cgatggcgcg gccgatgccg gtgaagtcgt cggcgacgag
      181 gcggaacgaa tcggggtctt cgagtgcggt gtcgacgccg agcgagacga cgagcgcgtc
      241 cggcgcgaag tgcgtgatcg ccgcgttcgc gtcggcaaac gcggcgagat acgcgtcgcc
      301 cccggtaccg gcgggcaggg gaaagttgcg cgtgaagcct tcacccgcgc cccagccctt
      361 ctcctcggag aacccgagga agtacgggta gtcctcggcc gggtccgcgt gcagcgacgt
      421 gaacaacacg tcgtcgcgcg catagaagat ctgctgggtg ccgttgccgt ggtggaagtc
      481 gacgtcgagc agcgccacac gcgcaccggc gtcgacccac gcctgcgcga cgatcgccgc
      541 gttgttcagg tagcagtagc ccgcgaaccc gtcggcggcc gcgtgatgac cgggcgggcg
      601 gcacaacgcg tacgcatggg cggcaccttc ttcggcgacc gcccgccacg cggtcaacgc
      661 gacgtcgact gcgcccgttg ccgcggtcca cgtgcccgcg aggatcgggt cggagtcgtg
      721 ggagtaccaa ccgagccggg cgatgatgtg atgcgcgtcg ttgtggggtt ggtctcgtac
      781 cggtcgtgcg tacggcactg cttctgcgtc gtcgagctcg ccggtcgcgg cctgccaatc
      841 ggtgaacgcg gtcgcgagga actcgacgta gtcggtcgtg tgcacgcgca gcaacggttc
      901 gggtccgaac tcgtccggtt cgcgcacagg gccgagcgcc gcctcgtcga tcgcggcccg
      961 gatgcgttcc gcgcgctcgg ggatctcggg cggaggcacc aagcgaccgc cgtcgtggat
     1021 gggttggtgc ggatgatgga cgcgatgcac gtccgacgcg atcgtcaaca acgcgagtgc
     1081 acgctaaccg cggctcacga acgagaagca gaacgcctcc agcgcgctgc gagcgcgcgc
     1141 ggggtcgacg ttgggcggga cgagtccgtc gcccgagaac cgccggcgcg cgttcgtgtt
     1201 ctcgcgcgcg agcgcgagct cgagactgtg gcgcaccaca cccgtgagcg cggtgtcgga
     1261 cgtgtcgggc acgacgtgga ggtcgaagtc gcaaccggcg agccgcccgc gtacggcacc
     1321 gaacgcctcg cgatcgagcg gcgcgacgaa cctcgcgccg aggtcgcgag ccaggaggtg
     1381 cagccgtgca ttcgtgcgcc cctggagccg gagccacccg agggcatcat ctcgctgtcg
     1441 ccggcccatc accgcacagc attgacgaca gcggccgcca aggaaagccc tacgcttggc
     1501 gaatgccgtc ggtacaggtc tggcgggtcg acctggacca gccgccgccc gctgtcacgg
     1561 ccctcgcaac ggtgctcgac gaggacgagc gagcggcggc cgccaagcgg gtcggctcgg
     1621 tccgcgatcg ctacgtcgtc gcccacggcg cgctgcggac ggtgctggcg cacgtcgcgg
     1681 gcggcgcggc gggcgccctc gtgctcgacc ggacctgcgg gcactgcggc gatccgcgtc
     1741 acggtcgtcc gacgctggcc gggtcggacg tcgagttcaa cctctcgcac agcgacggca
     1801 tcgcgttgat cgcgtgcaca cacgcggtgc gcgtcggcgt cgacgtcgaa gtggtacggc
     1861 cgcgttctct gctcccgaag ctcgcgcggc gcgtgctgga tgaagagagc tacgaggcgt
     1921 ggctcgcgcg gcccgaggcc gagcagctcg gtgtgttcat ccaggagtgg acggcgcgtg
     1981 aggcctacct caagggcgtc ggtctcggca tcgtgcggcg gctccgtgac gttcccgcgt
     2041 cgccgccggg ctggacgatc accgcgctcc ccgtcggcga gctggcccgc gccagcctcg
     2101 cggtcgagca tcccgatccg cccggcgtcc gactcgcgac gtggtcgctc gcgggcaccg
     2161 gtaccctcga gcgatgaacg aggacgcgtt cgacgcgccc gacgcagacg aggtcgacgc
     2221 cg
//
LOCUS       DASUGA010000770         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1337553, whole genome shotgun sequence.
ACCESSION   DASUGA010000770 DASUGA010000000
VERSION     DASUGA010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337553"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VFX21_15130"
     CDS             <1..717
                     /locus_tag="VFX21_15130"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX5097359.1"
                     /translation="LRPVVVVALTGRRLGRTDKFPYSKAPTLPRAYFDAVERAGGVPL
                     LVERAPDPKDLLARVDALVLTGGPDVDPARYGQERHPSVYGVDGAIDEFECDLVDAAT
                     ARSVPVLAICRGIQVLNVARGGTLFQHVPEHPGVPAHGHPGEAGGAREHVVQLERGSL
                     VAEVMEAAHVMASCHHHQAIDALGDGLSVVGRAADGIVEAAELDGAFVLAVQWHPEDT
                     APTDKAQQRLFDALIRKAAK"
     gene            complement(735..>2124)
                     /locus_tag="VFX21_15135"
     CDS             complement(735..>2124)
                     /locus_tag="VFX21_15135"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5097360.1"
                     /translation="QYKALVERAAAGLHDLGVRADDNVSWQLPTWTESAVLVGALCRL
                     GAVQNPMLPIYRHKEVSFIAKQTNCKLLVTPSSWNKFEYAALAEQVAGENDGMHTLVA
                     DHWNPEGDPASLPAFVSAPDATRWIFYTSGTTADPKGARHTDRSVLAGAIGYAHKTHV
                     VENDIALVAFPFTHVGGIIIGVFTPLLTGSAAVLMEVWTPQASTELIAKHGITLGNGA
                     PAIHTALLSEIQANPNAYKTIRGFPSGGSTKPPQLHDQLRDAVPGSFGITSGYGMTEA
                     PIVSQTDIDAPEESKRNGEGTATPGVEFKIADNGELLVKGPQVMQGYVDSSLDKDAFT
                     ADGWLHTGDMARFDEYGAVLITGRIKDIIIRKGENVSAKEVEDVLYTHPNIADVAVLG
                     IPDADRGEMVVAFIVPKDGSDAPGVRELFEFCKGVGLMTQKIPERVEIIDVMPRNPSG
                     KVPKHELRARIT"
BASE COUNT          312 a          724 c          729 g          359 t
ORIGIN      
        1 cttcgaccag tggtagtcgt cgcgttgacg ggtcgccggt tggggcggac cgacaagttt
       61 ccctactcga aggcgcccac gttgccgcgc gcgtacttcg acgcggtcga gcgtgcaggt
      121 ggtgtcccgc tcctggtgga acgcgcgccc gacccgaagg atctgctggc gcgcgtcgac
      181 gcgctggtgc tcacgggtgg tcccgacgtc gatccggcgc gctacggcca ggagcggcat
      241 ccgtcggtgt acggcgtcga cggcgcaatc gacgagttcg agtgcgactt ggtcgacgcc
      301 gcgacggcgc gctcggtccc ggtgctcgcg atctgccgcg gcatccaggt gttgaacgtc
      361 gcgcgcggcg gcacactgtt ccagcacgtt ccggagcatc ccggcgtacc cgcgcacgga
      421 catccgggcg aggccggcgg cgcgcgtgag cacgtggtgc agctcgagcg cggttcgctc
      481 gtcgccgagg tcatggaggc ggcccacgtc atggcgtcgt gccatcacca ccaggcgatc
      541 gacgcgctcg gcgacggctt gtcggtggtg ggccgcgccg cggacggcat cgtcgaagcg
      601 gccgagctcg acggcgcgtt cgtcctcgcc gtccagtggc accccgaaga caccgccccc
      661 accgacaagg cgcaacaacg cctgttcgac gccctcatcc gcaaggcggc caagtagaag
      721 gttggtcggg caactcaggt gatgcgggct cgcagctcgt gcttggggac cttgccgctc
      781 gggttgcggg gcatgacgtc gatgatctcc acgcgctcgg ggatcttctg ggtcatcaag
      841 ccgacgccct tgcagaactc gaacagttcc cgaacgccgg gcgcgtcgga gccgtccttc
      901 ggcacgatga acgcgacgac catctcgccg cggtcggcgt cggggatgcc gagcaccgcg
      961 acatccgcga tgttcgggtg cgtgtagagg acgtcctcga cttccttggc ggagacgttc
     1021 tcgcccttgc ggatgatgat gtccttgatg cggccggtga tgagcaccgc gccgtactcg
     1081 tcgaagcgcg ccatgtcgcc ggtgtgcaac cacccgtcgg ccgtgaacgc gtccttgtcg
     1141 agcgacgagt cgacgtagcc ctgcatgacc tgcggaccct tgacgagcag ctcgccgttg
     1201 tcggcgatct tgaactcgac gcccggcgtc gcggtgcctt cgccgttgcg cttcgactcc
     1261 tcgggtgcgt cgatgtcggt ttgactgacg atcggcgcct cggtcatgcc gtagcccgac
     1321 gtgatgccga acgaaccggg caccgcgtcg cgcagctggt cgtgcagctg cggcggcttc
     1381 gtcgaaccgc ccgacgggaa gccgcggatc gtcttgtacg cgttcgggtt cgcctggatc
     1441 tcgctcagca gcgcggtgtg aatcgcgggc gcaccgttcc ccaacgtaat cccgtgcttg
     1501 gcgatcagct cggtcgacgc ctgcggcgtc cacacctcca tgagcacggc ggccgaaccg
     1561 gtgagcagcg gcgtgaacac accgatgatg atgccgccga cgtgcgtgaa cgggaacgcg
     1621 acgagcgcga tgtcgttctc gacgacatgc gtcttgtgcg cgtacccgat cgcaccggcg
     1681 agcaccgagc gatccgtgtg acgcgcgccc ttcgggtcgg cggtcgtgcc cgacgtgtag
     1741 aagatccacc gcgtcgcgtc gggcgcggac acgaacgcgg gcaacgacgc gggatcaccc
     1801 tcggggttcc agtggtcggc gacgagcgtg tgcatgccgt cgttctcgcc cgcgacctgc
     1861 tccgccagcg ccgcgtactc gaacttgttc cacgacgacg gcgtgacgag caacttgcag
     1921 ttcgtctgct tcgcgatgaa cgacacttcc ttgtgccggt agatcggcag catcggattc
     1981 tgcacggcac ccaaccggca cagcgcgccc accaacaccg cggactccgt ccacgtcggc
     2041 agctgccacg acacgttgtc gtcggcgcgg acaccgaggt cgtgcagccc ggccgcggcg
     2101 cgctcgacga gcgccttgta ctgg
//
LOCUS       DASUGA010000771         2245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1476244, whole genome shotgun sequence.
ACCESSION   DASUGA010000771 DASUGA010000000
VERSION     DASUGA010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476244"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2106
                     /locus_tag="VFX21_15140"
     CDS             <1..2106
                     /locus_tag="VFX21_15140"
                     /inference="COORDINATES: protein motif:HMM:NF040071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HEX5097361.1"
                     /translation="VCVVVSLALAACTGDDDDSSSATEATGSSSTEAAGNAVDDGART
                     RVTEGALTVESTSTRAGLVSDDDVLVTVTGASGDLQVELGDRDISDAFTASDDGAVGL
                     VTDLPDGDSDIIVTSGDDTATLTVTNHSKNGPVFSGPHLEPWVCTTEAHGLGAPTDED
                     CDAPSKTEWSYVATDGSVRALDDPSSAPADVATATVDDKQVPFIVRTETGVIDRGIYT
                     IIVVDPDPTASKWSAEGWNERLVYRFGGGCGTGYTQGEGLLTDGIDTDLLGRGYALAT
                     NTLDTFQSDCNAVLSAEAALMTREHFIEQYGVPQYTIGDGGSGGAIQQLLTAHNYPGI
                     LDALSPSVPFPDAVSISGAVSDCGLLHHYYESEAGTALTDAQRAAIDGQATTGTCDLW
                     ITSFLGAIEPSNGCSPELDGQTYDAETRPDGVRCTLADINVNYLGRDEKTGFANRPLD
                     NVGIEYGRGVLEDGTITVDQFLDLNEQIGGYDIDGNFVADRESASDDVISIAYKTGAV
                     IGEGPLQEIPILLRNVYTDLLGDIHTRVQSFSIRDRLATDDVDDPNLLLWTSPSSGNL
                     TQTLTGQIGNANDPISVLDEWLTTGKKPATATNRCTLTDGTVIEGGWDIYDEPGPCAD
                     SFPVYSSSRIVAGQERDQFVIKCRLQPVDASSYGVSFTEEQAQRLAAIFPDGVCDWSK
                     PGAGAQAPASTWQSFGSGS"
BASE COUNT          401 a          753 c          736 g          355 t
ORIGIN      
        1 gtatgcgttg tcgtcagcct cgcgctggcc gcgtgcaccg gtgacgacga cgactcgtcg
       61 tcggcgaccg aggccaccgg ctcgagctcg acggaagctg ccgggaacgc ggtcgacgac
      121 ggcgcgcgca cacgggtgac cgagggtgcg ctgacggtcg agagcacctc gacacgcgcc
      181 ggtctcgtga gtgacgacga cgtgctcgtg accgtcaccg gtgcctcggg cgaccttcag
      241 gtggagctcg gcgaccgaga catcagcgac gcgttcactg cgtcggacga cggcgcggtc
      301 ggactcgtca ccgaccttcc ggacggtgac agcgacatca tcgtcacgtc cggcgatgac
      361 accgcgaccc tcacggtgac gaaccactcg aagaacggtc ccgtgttctc gggaccgcac
      421 ctcgaaccgt gggtctgtac gaccgaggcg cacggcctcg gcgcgccgac cgatgaggac
      481 tgtgacgcgc cgtcgaagac cgagtggtcg tatgtggcca ctgacggatc cgtacgcgcg
      541 ctcgacgatc cttcgtcggc ccccgccgat gtcgcaacgg caaccgtcga cgacaagcaa
      601 gtgccattca tcgtgcggac cgagaccggt gtgatcgacc gcggtatcta cacgatcatc
      661 gtcgtcgatc ccgatcccac tgcctccaag tggagcgccg agggatggaa cgaacggctt
      721 gtataccgct tcggcggtgg ctgcggcacc gggtacacgc aaggcgaggg actcttgacc
      781 gacgggatcg acacagatct gctcggccgt gggtacgcgc tcgcgacgaa cacgctcgac
      841 acgttccaga gcgactgcaa cgccgtcttg tcggccgaag ccgcgctcat gacacgcgaa
      901 cacttcatcg agcagtacgg cgtgccgcag tacacgatcg gtgacggtgg ttcgggcggc
      961 gcgattcagc agctgctgac tgcgcacaac tatccgggca tcctcgacgc gctctcgccc
     1021 agcgtgccgt tccccgatgc ggtatcgatc tccggcgcgg tgagcgactg cggtctgctg
     1081 catcactatt acgaatcgga agcgggcacg gcgctcacgg acgcgcaacg cgccgcgatc
     1141 gacggtcagg ccacgaccgg aacgtgtgat ctctggatca cttcgttcct cggcgcgatc
     1201 gaaccgagca acggctgctc gcccgagctc gacggtcaga cctacgacgc cgagacgcgg
     1261 ccggacggcg tgagatgcac gctcgccgac atcaacgtca actatctcgg acgcgacgag
     1321 aagaccggct ttgccaaccg gccactcgac aacgtcggca tcgaatacgg ccgcggtgtg
     1381 ttggaggacg ggacgatcac ggtcgaccag ttcctcgatc tgaacgagca aatcggcggc
     1441 tacgacatcg acggcaattt cgtcgccgat cgcgagtcgg cgagcgacga tgtcatctcg
     1501 atcgcataca agaccggtgc ggtgatcggt gagggaccgc tgcaggagat tccgatcttg
     1561 ttgcgcaacg tctacaccga tctactcggc gacattcaca cgcgcgtgca gtccttctcg
     1621 atacgcgacc gtctcgcgac cgacgacgtc gacgacccga atctcctgct ctggacgtcg
     1681 ccgtcgtcgg gcaacctcac gcagacgctg accggacaga tcggcaatgc caacgacccg
     1741 atctcggttc tcgacgagtg gctcacgacg ggcaagaagc ccgcgacggc aacgaaccgt
     1801 tgcacgctca cggacggcac cgtgatcgaa ggcggctggg acatctacga cgagcccggg
     1861 ccgtgcgccg actcgttccc ggtgtacagc agctcgcgca tcgttgccgg gcaggagcgc
     1921 gaccagttcg tcatcaagtg ccggctccaa ccggtcgacg cgtcgtcgta cggcgtgagc
     1981 ttcacggagg agcaggcgca gcgactcgcg gcgatcttcc ccgacggcgt atgcgattgg
     2041 tccaagcccg gcgccggcgc gcaggcgccg gcgtcgacgt ggcagtcgtt cgggagtggg
     2101 tcgtaggcga tcccgcgtcc gctttgcccc gtgcggcgag cgccgcgatg acaccagcta
     2161 ccttccggcg tgtgcgggtc ctcagcgtca tcgacagtct cgacgccggc ggtggcgccg
     2221 agcggtcgct ggcggcgctt gcccc
//
LOCUS       DASUGA010000772         2299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_191776, whole genome shotgun sequence.
ACCESSION   DASUGA010000772 DASUGA010000000
VERSION     DASUGA010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191776"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VFX21_15145"
     CDS             complement(<1..335)
                     /locus_tag="VFX21_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097362.1"
                     /translation="MTLIETEDEELDLAAPETRAAGRWNTVGVPGERSKDFRRALRRL
                     SHMLGRNWIVLGFVAFVAVVSATANVFGPKVLGHATDIIISGVSGGQGVDFGELHEVL
                     LEALALYAVS"
     gene            complement(332..2065)
                     /locus_tag="VFX21_15150"
     CDS             complement(332..2065)
                     /locus_tag="VFX21_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020638624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5097363.1"
                     /translation="MLMRLLRAHLAPYKRTLWLIVVLTIVHTSAALTLPTINARIIDD
                     GVLKGDNAYIRHWGAVMLGFAIVQVVFSVAAVYFGGKVAMSVGRDLRGGLFHRVTDFS
                     AREVGTFGAPSLITRITNDVQQVQMLVVMTCTMAISAPITMVVGVFFALRQDVQLSIV
                     LVFAMPIAGVVLGLIVSQMVPAFRLMQDRIDQVNRVLREQITGVRVVRAFVREPQERQ
                     RFSSANGDLTQVSLRAGRLMSSMFPTVNFLINASSVAVLWLGASLVNKGEIEVGTLVA
                     YLSYLVQILMSVVMATFTISMIPRASVSADRLQEVLDTETSVVPPADPVRELVAPGAL
                     EFSAVGFKYPGAEHAVLSDVSFATSPGTTTAIIGSTGAGKSTLVNLILRLFDSTDGSV
                     LVGGVDVRDLDPDILWSTIGYVPQRPYLFSGTVASNLQFGKPDATEEEMWAALDVAQA
                     SDFVRAMPGGLHARIEQGGTNVSGGQRQRLSIARALIRRPDIYVFDDSFSALDVATDA
                     RLRAALVPYTRDAAVIVVAQRVSTIRTADDILVLEDGEVIGRGTHDELMANSPTYAEI
                     VQSQIGEKSAA"
     gene            complement(2059..>2299)
                     /locus_tag="VFX21_15155"
     CDS             complement(2059..>2299)
                     /locus_tag="VFX21_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097364.1"
                     /translation="ASSIGTRFNEMARRPMADSDALVRLFERYRGEIRVEPVAAARLL
                     RALTLSTTHPMLADAPWSADELTQTFLHGVGAAAC"
BASE COUNT          401 a          762 c          790 g          346 t
ORIGIN      
        1 gacaccgcgt acaacgcgag cgcttccagc aggacttcgt gcaactcgcc gaagtcgacg
       61 ccctgcccgc cggagacgcc ggagatgatg atgtcggtgg cgtgaccgag gaccttcggg
      121 ccgaacacgt tcgccgtcgc gctgacgacc gccacgaacg cgacgaaccc gagcacgatc
      181 cagttccgcc cgagcatgtg actgaggcgc cggagtgcgc gccggaagtc cttcgaccgt
      241 tcgccgggga cgccgacggt gttccaacgg ccggcggcgc gggtctcggg cgcggcgaga
      301 tcgagttctt cgtcttccgt ttcgatgagc gtcatgccgc gctcttctcg ccgatttgcg
      361 actggacgat ctccgcgtag gtgggggagt tcgccatgag ctcgtcgtgg gtgccgcggc
      421 cgatgacctc accgtcctcg aggacgagga tgtcatcggc ggtgcggatc gtcgagacgc
      481 gctgcgcgac gacgatcacc gccgcgtcgc gcgtgtacgg caccagcgcg gcgcgcaacc
      541 gcgcgtcggt cgcgacgtcg agcgcggaga acgagtcgtc gaacacatag atgtcgggcc
      601 tgcggatcag cgcccgcgcg atcgagagtc gctggcgttg gccgccggag acgttcgtac
      661 caccctgctc gatgcgcgcg tgcaacccgc ccggcatggc gcggacgaag tcgctcgcct
      721 gggccacgtc gagcgcggcc cacatctctt cttccgtcgc gtcgggcttg ccgaactgca
      781 ggttgctggc aacggtgccc gagaagagat acggccgctg cgggacgtag ccgatcgtgc
      841 tccacaagat gtcgggatcg aggtcgcgca catcgacccc tccgacgagc acgctgccgt
      901 cggtgctgtc gaacaaccgc aggatgaggt tgacgagtgt ggacttgccc gcgcccgtgc
      961 tgccgatgat cgcggtcgtc gtgcccggtg aggtggcgaa cgagacatcg ctcagcaccg
     1021 cgtgctccgc gcccgggtac ttgaacccga cggcgctgaa ctcgagcgcg cccggcgcga
     1081 ccagctcgcg caccgggtcg gcgggcggca cgacgctggt ctcggtgtcg agcacttcct
     1141 ggaggcggtc ggcggacacc gacgcacgcg ggatcatcga gatcgtgaag gtcgccatca
     1201 ccaccgacat caggatctgc accaagtagg agaggtacgc gaccagcgtg ccgacttcga
     1261 tctcgccctt gttgacgaga ctcgcgccca accagagcac cgcgacgctc gacgcgttga
     1321 tcaggaagtt caccgtcggg aacatcgacg acatcagccg gccggcgcgc agcgacactt
     1381 gagtcaggtc accgttcgcc gacgagaatc gctgccgctc ctgcggttca cgcacgaacg
     1441 cgcgcacgac acggacaccg gtgatctgct cgcgcagcac gcggttgacc tggtcgatgc
     1501 ggtcctgcat gaggcggaac gcgggcacca tctgcgacac gatgaggccg agcaccacgc
     1561 ccgcgatcgg catcgcgaac acgagcacga tcgagagctg gacgtcttgg cgcagtgcga
     1621 agaacacgcc gacgaccatc gtgatcggcg ccgagatcgc catcgtgcac gtcatgacga
     1681 cgagcatctg cacttgctgg acgtcgttgg tgatgcgcgt gatcagcgac ggcgcaccga
     1741 acgtgccgac ttcacgggcc gagaagtcgg tgacacggtg gaagaggccg ccgcgcaagt
     1801 cgcggccgac gctcatggcc actttgccac cgaagtacac cgcggccacg gagaacacga
     1861 cctgcacgat cgcgaagccg agcatgacgg cgccccagtg gcggatgtac gcgttgtcgc
     1921 ccttcagaac accgtcgtcg atgatgcggg cgttgatcgt cggcagtgtc agcgcggccg
     1981 aggtgtgcac gatcgtcagc acgacgatca accacagcgt ccgcttgtag ggagcgagat
     2041 gggcgcgcag cagtcgcatc aacatgcggc cgctccgacg ccgtgcaaga aggtctgcgt
     2101 gagctcgtcg gccgaccagg gtgcgtccgc gagcatcggg tgcgtcgtcg acagcgtgag
     2161 tgcccgcagc aagcgcgcgg ccgcgaccgg ttcgacgcgg atctcgccgc ggtagcgctc
     2221 gaagaggcgc acgagtgcgt cgctgtcggc catcgggcgg cgcgccatct cgttgaagcg
     2281 ggtgccaatg ctcgacgcc
//
LOCUS       DASUGA010000773         2099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_210169, whole genome shotgun sequence.
ACCESSION   DASUGA010000773 DASUGA010000000
VERSION     DASUGA010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210169"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VFX21_15160"
     CDS             <1..246
                     /locus_tag="VFX21_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097365.1"
                     /translation="YDIVERSDFGGGGEGTVIRDLVRGRVRYVCLHPPRPRRKAESPL
                     VPTKLEQWVRTNYTYVGRYPACDLYRAAAGSVTGPRA"
     gene            complement(204..1376)
                     /locus_tag="VFX21_15165"
     CDS             complement(204..1376)
                     /locus_tag="VFX21_15165"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5097366.1"
                     /translation="MPASSVLYDPYAPDLRENPYPVYRYLRDEAPVYRHPDKGFFVVS
                     RFADVLETLYDFETYKSGAGITLEGLPPDVQPEMITMDPPRHDELRALVKRAFTPRAI
                     SALEPRVYEIARSLVGELDPESFDLVQDVGGLVPSAVIAEMLGIPFSDRPLFREWIEV
                     LIRRKPDEPDTIAAARAASAELSEYLAGQIAARRPVPTDDLIGYSVRVADESGAMNDE
                     ELLGFVRLLLVAGTETTINLIGNAFVTLARNPDERARVAADAGLTPSAVEEVLRFESP
                     VPQLCRTTTRDVVRYDTEIPSGSVVVMVFAAANRDEREFDEPDRFDATRRVDRHLALG
                     HGIHHCLGAALARLQARASIDAVLERFPTYEITTDTIEMLPSGHARGPVTLPAAAR"
     gene            complement(1382..>2099)
                     /locus_tag="VFX21_15170"
     CDS             complement(1382..>2099)
                     /locus_tag="VFX21_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HEX5097367.1"
                     /translation="DGDIRIASDPPLIVPVRELVDMSAVRDNEKWIHDRFVEYRRTLQ
                     PDRRHLLEGYRIVDMARKVVGVGSVGTRTWILLMLGKDDNDPLMLQIKEAAPSVLEPY
                     AGKSGYASNGQRVVEGQRLLQATSDILLGWIHVEGIDGVQRDYYVRQLWDGKMSPSFE
                     TMTPAALRLFAELCGRTLARGHARSGDRIAIAAYLGNGTAFDDAVVAFSNAYADLNDR
                     DYDAVTAAVKDGRLAATAAP"
BASE COUNT          316 a          702 c          744 g          337 t
ORIGIN      
        1 tatgacatcg tcgagcggtc cgacttcggt ggcggtggcg agggcacggt gatccgggac
       61 ctcgtccgcg gccgcgtgcg ctacgtgtgc ctgcacccac cgcggcctcg ccgcaaggcc
      121 gagagtcccc tcgtaccgac caagctggag cagtgggtgc ggacgaacta cacctacgtc
      181 ggtcgctatc cggcgtgcga cctctaccga gccgccgcgg ggagcgtcac cgggccgcgt
      241 gcgtgacccg acgggagcat ctcgatcgtg tcggtggtga tctcgtaggt cgggaagcgc
      301 tccagcaccg cgtcgatcga cgctcgcgcc tgtaggcgcg cgagcgccgc gccgaggcag
      361 tgatggattc cgtgacccaa cgcgaggtgg cgatcgaccc tgcgcgtcgc gtcgaagcgg
      421 tcgggttcgt cgaactcacg ctcatcgcgg ttggccgccg cgaacaccat cacgacgacg
      481 gagcccgatg ggatctcggt gtcgtagcgc acgacgtcgc gggtcgtggt gcgacacagc
      541 tgcggtaccg gtgactcgaa gcgcagcacc tcttcgactg cgctcggtgt gagcccggcg
      601 tcggccgcga cgcgggcacg ctcgtcgggg ttgcgagcga gcgtgacgaa cgcgttgccg
      661 atgaggttga tcgtcgtctc ggtgccggcg acgagcagca gccgcacgaa gccgagcagc
      721 tcttcgtcgt tcatggcgcc gctctcgtcg gcgacccgca ccgagtagcc gatgaggtcg
      781 tcggtgggga ccggccgccg cgccgcgatc tgacccgcga ggtactcgga gagctcggcg
      841 ctggcggcgc gtgcggcggc gatcgtgtcg ggctcgtcgg gcttgcggcg gatcagcacc
      901 tcgatccact cacggaacag tgggcggtcg gagaacggga tcccgagcat ctccgcgatc
      961 accgccgacg gtacgagccc gccgacgtcc tgcacgagat cgaacgactc gggatcgagc
     1021 tcgccgacga gcgaacgcgc gatctcgtag acgcgcggtt ccagtgccga gatcgcacgg
     1081 ggggtgaacg cgcgtttcac cagcgcgcgc aactcgtcgt ggcgcggcgg gtccatcgtg
     1141 atcatctcgg gttgcacgtc gggcggcagc ccctcgagcg tgatgccagc acccgacttg
     1201 taggtctcga agtcgtagag cgtctccagc acgtcggcga accgactgac cacgaagaag
     1261 cccttgtccg ggtggcggta cacgggtgcc tcgtcgcgca ggtagcggta gaccgggtac
     1321 gggttctcgc gcaggtcggg cgcgtacggg tcgtagagga cgctcgacgc gggcatcgcc
     1381 gtcacggggc cgcagtcgcc gcgagtcgcc cgtccttcac cgccgcggtc accgcgtcgt
     1441 aatcacggtc gttgagatcc gcgtacgcgt tcgagaacgc cacgaccgcg tcgtcgaacg
     1501 cagtgccgtt cccgaggtac gccgcgatcg cgatgcgatc gccggagcgc gcgtgaccac
     1561 gcgccagtgt gcgtccgcac agctcggcga agagccgcag cgccgcgggc gtcatcgtct
     1621 cgaagctcgg cgacatcttc ccgtcccaga gctggcgcac gtagtagtcg cgttgcacgc
     1681 cgtcgatgcc ttcgacgtgg atccagccga ggaggatgtc gctcgtcgcc tggaggagcc
     1741 gctgcccctc cacgacgcgc tgaccgttgc tcgcgtaacc gctcttgccg gcatacggtt
     1801 cgagcaccga cggtgcggcc tccttgatct ggagcatgag cgggtcgttg tcgtccttgc
     1861 cgagcatcag caggatccac gtgcgcgtgc cgacgctgcc gacgccgacg accttccgcg
     1921 ccatgtcgac gatgcgatag ccctcgagga ggtgacggcg gtccggttgc aacgtgcggc
     1981 ggtactccac gaagcggtcg tggatccatt tctcgttgtc acgcaccgcc gacatgtcga
     2041 cgagctcacg gaccggcacg atgagcggcg ggtcgctggc gatccggatg tcgccgtcg
//
LOCUS       DASUGA010000774         8124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_17777, whole genome shotgun sequence.
ACCESSION   DASUGA010000774 DASUGA010000000
VERSION     DASUGA010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8124
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17777"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VFX21_15175"
     CDS             complement(<1..812)
                     /locus_tag="VFX21_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097368.1"
                     /translation="MTRRDERGSTLIIALAMIAVVGLIITSGVQFSGVSLRSSNNALR
                     PNRASLYAADGALQTAIEFVRDNPGAAADLLGACPANTLTYPDPGSGSTVSVALCPQD
                     GSFEYDGPYQAVLVTLSTRADEGLDLSHNGDVDIGGHVWSNSYINLSNPTNAIVKGGN
                     VYAWTSCNRPGGITVDPGFVKNCNASAAVGAVPEVGKDPAIANPADWTPAVPASFVPQ
                     TVATCSGGAATLTPGIYEHGKDLSALTKSCNNVTLSPGVYYLNVPADGTGGSL"
     gene            complement(809..1312)
                     /locus_tag="VFX21_15180"
     CDS             complement(809..1312)
                     /locus_tag="VFX21_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX5097369.1"
                     /translation="MTTTRRDAGGFTLVELLITVVLAGIIMGPLAGALFVGFRSTADT
                     RTSLEQSNAEQLVSTYVAKDIQAAEQVSYDPAGVCGGRVQFWMRSTAMAGSADTVVAY
                     SLDGTTLSRLECDTDGNQVNTHVLAENVSQFDPSGSSTVHVSVTTAGSTSVAAYSWSI
                     DVARRLP"
     gene            complement(1309..1719)
                     /locus_tag="VFX21_15185"
     CDS             complement(1309..1719)
                     /locus_tag="VFX21_15185"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX5097370.1"
                     /translation="MNRQYGGARDQRGTTLVEILITITIIGIGFVAVLGAIAVFHRST
                     GTQRGTATNDSAVRTYAEQLQAAPYSDCASAYNGVSAPSGFTPTVVVSYWSGDAAGTY
                     SSNCSGDRGTQRLQVTLTDAGGRADTIYVVKRKP"
     gene            complement(1716..2936)
                     /locus_tag="VFX21_15190"
     CDS             complement(1716..2936)
                     /locus_tag="VFX21_15190"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5097371.1"
                     /translation="MPTFQYVAVDEQGERIKRKVDARDESALRKELLRRNYEVKSIKS
                     KKAKARPFERHKVKASEIMHFSRQMSAFMRSGISITDGLDVIAKATKNRYFGEVLYGM
                     REQIRQGTPFADALARHAGIFPPYYIGILRSAELTGHLDRALDELAGYISRDLEAKRK
                     VKSATVYPAIVLLMSIVTVAILTVWVLPKFAVFFKDLDAKLPVTTRLMITVSDTSRQL
                     WFVYVLLLVLLAGVVYVFKSTTGGRRLRDRILLKIPVVGDIVLYSVVERVCRVLSAMT
                     QGGVPMPDAMTAAVAGANNAVFEEGLQPARERMLEGEGLAGPIEASQLFPDAAVQMIR
                     VGETTGTIGQQLDNAAEYYSRELDERLDRLTALFEPAVIIVMGVVVGFVAIALVQAMY
                     GIYNSSSLTGGVHP"
     gene            complement(2948..4759)
                     /locus_tag="VFX21_15195"
     CDS             complement(2948..4759)
                     /locus_tag="VFX21_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016573319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX5097372.1"
                     /translation="MRPTRTRKAAMQEFEAVMAARFTPPSDDANVDEDVRPAPDKTPL
                     GQLLIGQGRLTEEQLAEALLAKASNGIRFGEALVQTGAISPLDLAQALGAQLGFDVVD
                     LAHEEVDSDVAGRLPEDLARRLCAIPVRMHGERVLVATADPAVDGLRQDLIDALEAPV
                     RLLIAPSDQIQRAIDQTYRSQAEITDALRDFEERAKAQQRTVSTFDLAAALEADEDAP
                     VVRVVNLILEQGARDRASDIHIEPQPDRVRIRLRTDGAMTEMMSLPASMATSLVSRIK
                     VLSDMNIVERRKPQDGQMNFTIDGRELDIRVATSLTVHGEMCVMRLLDKSRALKQLSE
                     LGMPADTLERYRSLIRSPYGMVICAGPTGSGKTTTLYATLAEVNDDAIKVTTIEDPVE
                     YVIPSINQIQINELAGVTFAGGLRAILRQDPDSILVGEIRDVETARIAVQAALTGHFV
                     MSSLHATDASAALHRFLEMGIEPFVLTSSLLGVVGQRLVRRNCRHCTERYEPSAEELS
                     FFGDYASYDASDVVHRRGAGCNFCSHTGYFERVGVYEVLKVTEEMRELVAGGAQHHDI
                     RALAVEQGMRSLRDQAARLVADGTTTIAEVLRTVYVL"
     gene            complement(4888..5448)
                     /gene="orn"
                     /locus_tag="VFX21_15200"
     CDS             complement(4888..5448)
                     /gene="orn"
                     /locus_tag="VFX21_15200"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020525474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEX5097373.1"
                     /translation="MNTPDALVWIDLEMTGLDPDRDSIVEVACVVTDADLVAVDAMHC
                     VIHESDEVLAGMEAIVHDMHMASGLLDEIRESVVDVRFAEQLLLAYVGRYAAPRTAPL
                     CGNSIGTDRRFISRHMPAFDRYLHYRNVDVSSIKELCRRWRPDLYGALPDKGNQHRAL
                     EDVHDSIRELRFYREQIFGFTMPLPV"
     gene            complement(5576..6187)
                     /locus_tag="VFX21_15205"
     CDS             complement(5576..6187)
                     /locus_tag="VFX21_15205"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX5097374.1"
                     /translation="MQDLVGARVLLRPLRADDWDAWREVRVRCREWLERWEPLPDPGS
                     ADPVLERDAFRARCGAWERQRHFDAAYGFGMFLTNGRFAGEVSLGSVQRGPFQMGYIG
                     YWIDADLAGHGYTPEGVVVLMQYAFEVLTLHRLEAAIVPRNQASRRVAEKLGLREEGT
                     AQRFLQIQGVFEDHVRYAITTEEWQTRGHELVARFLTPTGDHA"
     gene            complement(6187..7425)
                     /locus_tag="VFX21_15210"
     CDS             complement(6187..7425)
                     /locus_tag="VFX21_15210"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5097375.1"
                     /translation="MTAGRDVTLDELTAGRADRFDAVVVDSEHPLFVAYTSGTTGRPK
                     GAVHVHGGFLVKIAQEVVFQMDLRAGERLFWLTDIGWIMGPWEIVGTLAGRGTLVLYD
                     GAPDQPGPDRLWDLVERRRVHILGASPTLVRALMAHGDAPVRAHDLSSLRVLGSTGEP
                     WNEAPWQWYFDVVGGGRCPVINISGGTEVGACFLSPHVVQALSACSLGGPALGMAVDV
                     FDDAGHSVRGDVGELVCTKPWPGMTRGLWRDPERYLETYWSRYPGVWWHGDFASVSDD
                     GQWFLHGRSDDTIKVAGKRLGPAEVETVLVAHPAVIEAAAIGVPDDVKGEALWAYVVL
                     VHDARADDALRDELRALVVEHLGPSFRPAQIRFTTALPKTRSAKILRRAIRSVVTGTP
                     PGDLSGLEDPNALDAIAAAG"
     gene            7441..8097
                     /locus_tag="VFX21_15215"
     CDS             7441..8097
                     /locus_tag="VFX21_15215"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5097376.1"
                     /translation="MQARDDDNRGTGYSRCAPMPASIATMADDAAAVLQAVGAEPALV
                     VGLSMGGMIAQELALRHPERVRALVLVGTRPPAPVHLQVPGDVISRLLRRPEPGETVG
                     QFLTATWATLCGPGFAAARPDLLDELVEQILVRTTPKASTFRQMSSIAAWHDPRRLAG
                     IAAPTTVVHGDVDPLMPVGNGTRLACLIPGARYVELAGVGHLPPLEASDAVAEIVEQV
                     "
BASE COUNT         1300 a         2792 c         2714 g         1318 t
ORIGIN      
        1 agactccccc cggtgccgtc ggccgggacg ttcaggtagt acacgccggg tgacaacgtc
       61 acgttgttgc acgacttcgt gagcgcggag aggtccttgc cgtgctcgta gattcccggc
      121 gtcagcgtcg ccgccccgcc cgagcaggtc gccaccgtct gcggcacgaa ggacgcgggg
      181 accgccggcg tccagtcggc cgggttcgcg atcgcgggat ccttgccgac ctcgggcacg
      241 gcgccgaccg cggcgctcgc gttgcagttc ttcacgaacc ccggatcgac ggtgatgcca
      301 ccgggccggt tgcacgacgt ccaggcatac acgttcccgc ccttgacgat cgcgttcgtc
      361 gggttggaca agttgatgta ggagttcgac cacacgtggc cgccgatgtc gacgtcgccg
      421 ttgtgcgaga gatcgaggcc ttcgtcggcg cgggtgctga gcgtgaccag caccgcttgg
      481 tacgggccgt cgtattcgaa cgagccgtct tgcgggcaca gcgcgaccga aaccgtcgaa
      541 cccgagcccg ggtcggggta cgtgagcgtg ttcgccgggc aggcgccgag gagatcggcg
      601 gccgcgcccg gattgtcgcg gacgaattcg atcgcggtct gcagcgcgcc gtcggccgcg
      661 tacagcgacg cccggttcgg tcgcagcgcg ttgttggagc tgcgcaggct cacgccgctg
      721 aactggacgc ccgacgtgat gatcaatccc acgaccgcga tcatcgccag ggcgatgatg
      781 agcgtgctcc cccgttcgtc ccggcgggtc atggcagtcg cctcgcgacg tcgatcgacc
      841 acgaatacgc cgccaccgac gtgctcccgg cagttgtcac ggacacgtgc accgtcgacg
      901 aaccgctcgg gtcgaactgg gagacgttct cggccaacac gtgcgtgttc acctggttgc
      961 catcggtgtc gcactcgagt cgcgagaggg tcgtcccgtc caacgagtac gccacgacgg
     1021 tgtcggcact tcccgccatg gcggtggaac gcatccaaaa ctggacacgg ccgccgcata
     1081 cgcccgccgg gtcgtacgac acttgctcgg ccgcttgaat gtccttcgcg acgtaggtcg
     1141 agacgagctg ctcggcgttg ctctgttcga ggctcgtccg cgtgtcggcg gtcgagcgga
     1201 atccgacgaa cagcgcgccc gcgagcggac ccatgatgat gccggcgagc acgaccgtga
     1261 tcaggagttc cacgagcgtg aagccgccgg cgtcgcggcg ggtcgtggtc atggtttgcg
     1321 cttcacgacg tagatggtgt ccgcgcggcc gccggcatcg gtcagtgtca cctgcagccg
     1381 ttgcgtccct ctgtcgccgg aacagttcga cgagtacgtg cccgcggcgt cgccgctcca
     1441 atacgacacg acgacggtgg gagtgaagcc actgggagcc gagacgccgt tgtacgcgga
     1501 cgcgcaatcg ctgtagggcg ccgcctgcag ttgttcggca taggtgcgga cggcactgtc
     1561 gttggtcgcc gtcccgcgct gggtcccggt cgaccggtgg aagaccgcga tcgcaccgag
     1621 gacggcgacg aacccgatac cgatgatcgt gatcgtgatg aggatctcga ccagcgtggt
     1681 cccgcgttgg tctcgggcgc cgccgtactg ccggttcacg gatggacacc gccggtgagt
     1741 gatgacgagt tgtagatgcc gtacatcgct tgcacgagtg cgatcgcgac gaagccgacc
     1801 acgacaccca tcacgatgat gaccgccggc tcgaacaatg ccgtgagacg gtccagccgt
     1861 tcatccagct ctcgcgagta gtactcggcc gcgttgtcga gctgctgccc gatggttccg
     1921 gtcgtctctc cgactcgaat catctggacc gctgcgtccg ggaacaactg cgacgcttcg
     1981 atcggtcctg cgagcccttc gccctcgagc atccgttccc gcgccggttg cagtccttcc
     2041 tcgaagaccg cgttgttggc gccggcgacg gcagccgtca tcgcgtccgg catcggcacg
     2101 ccgccttgtg tcatcgcgct caggacccgg cagacgcgtt cgaccaccga atacaggacg
     2161 atgtcgccga caacggggat cttgagcagg atccggtcgc gcaggcgccg cccgccggtt
     2221 gtgctcttga agacgtacac gacgccggcg agcaggacga gcaggagcac gtagacgaac
     2281 cacagctggc gcgatgtgtc ggagacggtg atcatcaacc gcgtggtgac cggcagcttc
     2341 gcgtcgaggt ccttgaagaa caccgcgaac ttcggcaaca cccacacagt gaggatcgcg
     2401 acggtgacga tggacatcag cagcacgatc gccggataca ccgtcgccga cttcaccttg
     2461 cgcttggcct cgagatcgcg gctgatgtag cccgcgagct cgtcgagcgc ccgatcgagg
     2521 tgacccgtca gttccgccga acggagaatc ccgatgtagt acggcgggaa gatgcccgcg
     2581 tgccgagcga gcgcgtcggc aaagggggtc ccctgccgga tctgctcccg cattccgtac
     2641 agcacctcgc cgaagtaccg gttcttcgtc gccttcgcga tgacgtcgag accgtcggtg
     2701 atcgaaatcc cgctccgcat gaacgcggac atctgccggg agaagtgcat gatctccgac
     2761 gccttgacct tgtgccgctc gaacggtcgc gccttcgcct tcttggactt gatcgacttc
     2821 acctcgtagt tgcggcgcag cagctccttg cgcagcgcgg actcgtcacg cgcgtcgacc
     2881 tttcgcttga tgcgctcgcc ctgttcgtcg accgcgacgt attgaaatgt cggcacggtg
     2941 tctctcccta cagcacgtag acggtccgga ggacctctgc gatggtggtc gtgccgtcgg
     3001 cgacgaggcg cgcggcttgg tctcggagtg agcgcatccc ctgctcgacc gcgagcgcgc
     3061 ggatgtcgtg gtgttgcgca ccaccggcga ccagttcccg catctcctcg gtcaccttga
     3121 gcacttcgta gacaccgacc cgttcgaagt agccggtgtg cgagcagaag ttgcagccgg
     3181 cgccgcgccg gtgcacgacg tccgaggcgt cgtacgacgc gtagtcgccg aagaacgaga
     3241 gctcctcggc actcggttcg taccgctcgg tgcagtggcg gcagttgcga cgcaccaggc
     3301 gctgcccgac cacgccgagc aacgacgacg tcaacacgaa cggctcgatg cccatctcca
     3361 ggaagcgatg gagtgcggcg ctcgcatcgg ttgcgtgcaa cgacgacatg acgaagtggc
     3421 ccgtcaacgc ggcctggacg gcgatgcgcg cggtctcgac atcacggatc tccccgacca
     3481 ggatcgagtc ggggtcctgc cgcaggatcg cccgcaggcc accggcgaag gtgacaccgg
     3541 ccaactcgtt gatctggatc tggttgatcg acggaatgac gtactcgacc ggatcctcga
     3601 tggtcgtgac cttgatcgcg tcgtcgttga cctcggccag cgtcgcgtag agcgtcgtcg
     3661 tcttgcccga accggtcggc ccggcacaga tgaccatgcc gtagggagaa cggatgagcg
     3721 accggtagcg ctccagcgtg tcggccggca tgccgagctc cgacagctgc ttcagcgccc
     3781 gactcttgtc caggaggcgc atgacacaca tctcgccgtg caccgtgagc gaggtcgcga
     3841 cccggatgtc gagctctcgg ccgtcgatcg tgaagttcat ctggccgtct tgcggcttgc
     3901 gccgctcgac gatgttcatg tccgagagca ccttgatacg cgacacgagc gacgtcgcca
     3961 tcgacgccgg cagcgacatc atctcggtca tcgcgccgtc cgttcgcaag cggatgcgca
     4021 cgcgatcggg ctgcggctcg atgtgtatgt cggacgcgcg gtcccgtgct ccttgctcca
     4081 ggatcaagtt gacgacccgc acgaccggcg cgtcctcgtc cgcctcgagc gccgccgcca
     4141 agtcgaaggt cgagaccgtt cgctgttgcg ccttcgcccg ctcctcgaag tcgcgcagag
     4201 catcggtgat ctcggcctgc gaccggtacg tctgatcgat cgcgcgctgg atctggtcgc
     4261 tcggcgcgat gagcagccgc accggcgcct cgagcgcgtc gatgaggtcc tggcgcaggc
     4321 cgtcgaccgc cggatcggca gtcgcgacga gcacgcgctc gccgtgcata cgaacgggga
     4381 tcgcgcagag acgccgggcc aagtcctccg gaagacgacc ggcgacgtcg ctgtcgacct
     4441 cttcgtgggc gagatcgacg acgtcgaagc cgagctgggc gccgagcgcc tgcgcgaggt
     4501 cgagcgggga gatggcaccc gtctggacga gcgcctcacc gaagcgaatg ccgttcgacg
     4561 ccttggcgag cagcgcttcg gccaactgct cctcggtcag ccgcccctgt ccgatcagga
     4621 gctgcccgag gggcgtcttg tcgggcgcgg gcctcacgtc ctcgtcgacg ttggcatcgt
     4681 cggacggcgg cgtgaaacga gcggccatca cagcttcgaa ctcctgcatt gccgccttgc
     4741 gggttcgcgt cggtcgcaat tcggttcacc tcgcgcctgt ccaccgcgcg gcagtcacgc
     4801 ggcatgcgga atctcttcgg cgctcgcagc ccggaacttc gaagtccacg gtgctttcgt
     4861 aaagaaacag aacccggcga gggagcgcta cacgggcagc ggcatcgtga agccgaagat
     4921 ctgctcgcga tagaaccgca gctctcggat cgagtcgtgg acgtcctcga gggcgcggtg
     4981 ctggtttccc ttgtccggga gggccccgta gagatcgggt cgccagcgac ggcacagctc
     5041 cttgatcgac gagacgtcga cgttccggta gtgcaggtag cggtcgaacg caggcatgtg
     5101 ccgggagatg aaccgacgat cggtgccgat gctgttgccg cagaggggcg cggtgcgcgg
     5161 cgcggcgtac cggccgacgt acgcgagcag gagttgctcg gcgaaccgca cgtcgaccac
     5221 ggactcccgg atctcgtcca acagtcccga agccatgtgc atgtcgtgca ctatggcctc
     5281 cataccggcg aggacctcgt cggactcgtg gatgacgcaa tgcatcgcgt cgaccgcgac
     5341 gaggtcggcg tcggtcacga cgcatgccac ttccacgatg ctgtcgcgat cgggatcgag
     5401 ccccgtcatc tcgaggtcga tccagacgag cgcgtccggg gtgttcaccg acttcagccc
     5461 tcctgcaggt gccccttctt gtcggcacgc catgcgcccg acctgacgca tcttcaggtt
     5521 ctcgagcggc tggatcgtcc gaattgcccg tcctgcgggg gtactcagca gtctcctagg
     5581 cgtggtcacc cgttggggtg aggaaccgag cgaccaactc gtggccgcgg gtctgccact
     5641 cctcggtcgt gatcgcgtag cgcacgtgat cctcgaacac gccctggatc tgcaagaacc
     5701 gttgggcggt tccttcttcg cgcagcccga gcttctcggc cacgcgccgg ctggcctggt
     5761 tgcggggcac gatcgccgct tcgagccggt gcagtgtcag cacctcgaac gcgtactgca
     5821 tcagcacgac aacgccttcg ggggtgtacc cgtgaccggc gaggtcggcg tcgatccaat
     5881 agccgatgta gcccatctgg aacgggccgc gctgcacgct gcccaagctc acctcaccgg
     5941 cgaagcggcc gttggtgagg aacatgccga agccgtaggc ggcgtcgaag tgccgttggc
     6001 gctcccacgc accacagcgc gcccggaacg catctcgttc gagtaccggg tccgcgctcc
     6061 cggggtcggg gagcggttcc caccgttcga gccactcgcg acagcgcacg cgaacctcac
     6121 gccacgcgtc ccagtcgtcg gcccgcaacg gccgcaacaa cacccgggca ccgacgaggt
     6181 cctgcatcag ccggcggccg cgatcgcgtc gagggcattg ggatcttcga ggccggacag
     6241 gtcgccgggc ggcgtccccg tcacgacgga ccggatcgcg cggcgcagga tcttcgcact
     6301 gcgcgtcttc ggcaacgcgg tcgtgaaccg gatctgtgcg ggccggaacg acggaccgag
     6361 gtgctcgacg accagtgccc gcaactcgtc gcgcaaggcg tcgtcggcac gtgcatcgtg
     6421 tacgagcacg acgtaggccc acaacgcctc gcccttcacg tcgtcgggca caccgatcgc
     6481 ggcggcttcg atcaccgcgg ggtgcgcgac gagcacggtc tcgacctccg cgggcccgag
     6541 ccgtttgccc gcgaccttga tcgtgtcgtc ggagcggccg tgcaggaacc attgcccgtc
     6601 gtcggagaca ctcgcgaagt caccgtgcca ccacacgccg ggatagcgcg accaatacgt
     6661 ctccagatag cgctcgggat ctcgccacaa tccgcgagtc atgcccggcc atggcttcgt
     6721 gcacaccagt tctccgacgt cgccccgcac cgagtgtccc gcgtcgtcga agacgtcgac
     6781 ggccatgccc agcgccggac cgcccagcga gcaggccgac aaggcctgca cgacgtgcgg
     6841 cgacaggaag cacgcgccga cctcggtccc gcccgagatg ttgatcaccg gacaccggcc
     6901 gccgcccacg acatcgaaat accactgcca cggcgcttcg ttccagggct cccccgtcga
     6961 cccgaggacc cgcagcgacg acaggtcgtg cgctcgcacc ggtgcgtcac cgtgcgccat
     7021 gagcgcccgc acgagcgtcg gcgacgcgcc gaggatgtgc acgcgccggc gttcgaccaa
     7081 gtcccacaaa cgatcggggc cgggctgatc aggcgcgccg tcgtagagga cgagcgtgcc
     7141 ccgaccggcg agcgtgccga cgatctccca cggccccatg atccaaccga tgtcggtcag
     7201 ccagaacaag cgctcgccgg cgcggaggtc catctggaac acgacctctt gcgcgatctt
     7261 gacgaggaag ccgccgtgca cgtgcaccgc acccttgggc cggcccgtcg tgccgctcgt
     7321 gtacgcgacg aacagcgggt gctcgctgtc gaccacgacc gcgtcgaaac gatcggcccg
     7381 tcccgcggtc agttcgtcga gggtgacgtc gcgcccggcg gtcatcggcg tgtcggcgcg
     7441 atgcaggcgc gggacgacga caaccgcggc accggctatt cgcgctgcgc gccgatgccg
     7501 gcgtcgatcg ccacgatggc cgacgacgcc gccgccgtgc tgcaggcggt cggggccgag
     7561 ccggcgctcg tggtcggctt gtcgatgggc gggatgatcg cccaggagct ggcgctgcgg
     7621 cacccggagc gggtgcgagc gctggtgctc gtgggcaccc ggccgccggc accggtccat
     7681 ctccaggtgc cgggcgacgt catcagccgc ctgttgcgcc ggcccgaacc gggcgagacg
     7741 gtcggccagt tcctgacggc cacatgggcc accctgtgcg gcccgggctt cgccgccgcc
     7801 cggcccgacc tcctcgacga gctggtcgag cagatcctcg tgcggacgac accgaaggcg
     7861 tccaccttcc gccagatgag ctccatcgcc gcgtggcacg acccgcggcg gctggcgggc
     7921 atcgccgccc cgacgacggt ggtgcacggc gacgtggacc cgctcatgcc agtgggcaac
     7981 ggcacgcgcc tggcctgcct catccccggc gcgcggtacg tggagctggc cggcgtcggc
     8041 cacctgcccc cactggaagc aagcgacgcg gtggccgaga tcgtcgagca ggtgtgaagt
     8101 atcttcgtcg ccgcctccgc tccg
//
LOCUS       DASUGA010000775         2868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1685295, whole genome shotgun sequence.
ACCESSION   DASUGA010000775 DASUGA010000000
VERSION     DASUGA010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685295"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFX21_15220"
     CDS             complement(<1..227)
                     /locus_tag="VFX21_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate CoA-transferase"
                     /protein_id="HEX5097377.1"
                     /translation="MTARELSVEEAAALVRPRDSLGVPLGPGIPGDFLHALSARDDWE
                     QLEVFGALLLDLYPLFTKPGVHYLSGFYGPA"
     gene            complement(224..709)
                     /locus_tag="VFX21_15225"
     CDS             complement(224..709)
                     /locus_tag="VFX21_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018177924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX5097378.1"
                     /translation="MTGEHEVIVEIPRGSQNKYEMDHDSGAIWLDRTLFTSMQYPADY
                     GFFPHTLADDGDPLDALVLLPEPTFAGCHVMSRPVAVFWMSDEKGDDAKVLCVPAHDD
                     RWDTVQDIGDIDPHVIAEIEHFFEFYKRIEPGKETETRGWEGAEAAERAIIEAKAAYP
                     G"
     gene            complement(706..1236)
                     /locus_tag="VFX21_15230"
     CDS             complement(706..1236)
                     /locus_tag="VFX21_15230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HEX5097379.1"
                     /translation="MELTLPPQLEEFVRSQVRSGRYADEQEVVREALRQMRSLVKAAE
                     DPSITGLVRDALGLANQAQKDVMTALASAEEETTVLGEVSRTAAAVASGAIDVARKVP
                     GAKEIERLVRGPVDSVAAVAEYGETQTKAVRQNLEASAKALGLLTAVLERVNGATRTV
                     NTVVGPRPPAQGQPPE"
     gene            complement(1247..2347)
                     /locus_tag="VFX21_15235"
     CDS             complement(1247..2347)
                     /locus_tag="VFX21_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007229384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEX5097380.1"
                     /translation="MRAALLEASEKPLAIVDDIDIQEPRAGEVMVRVTHCGVCHSDLS
                     AQNGTFPFTQPTVLGHEAAGIVEKVGTGVTSVRAGDKVVLTPVPSCNECYWCLRGEYG
                     CCVNAISLTTGAMLDGSTPLSRHGQPVMRGVGLGGFAEYVITPESGAVKVDDDTPLEV
                     ACVIGCAMQTGVGAVLNTAKVPEGATVFVAGLGGIGVAIVQGARIAGAAKIIVSDPVA
                     ERRATAKNFGATDVIDPTTTDVISTVQQLTSGIGVDYAFDAVGAGPVIESCLWSTRNG
                     GTTVMVGAGGIDQTVTLAPPVMFTLTERKIMGCLLGSCNGRRDIPRLLALYAAGRLDL
                     DGMITQRRPLADINDAFDDMTNGRGLRTVLTI"
     gene            2385..>2868
                     /locus_tag="VFX21_15240"
     CDS             2385..>2868
                     /locus_tag="VFX21_15240"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5097381.1"
                     /translation="MSTDIVDLWVNLVTEQSAEDFTSQEGYENIPGYLGSNTGPVGVP
                     SLIALMDELGVATGIFTTGIDRSTEKMLEICDANPGRFLVAAGVVDPSRPGRAVKRIR
                     ELAQHERLAMIRIMPLASQIPTNDARHYPIYQICEELGIPVAINAGIPGPRVRSKVQH
                     P"
BASE COUNT          452 a          973 c          956 g          487 t
ORIGIN      
        1 tcggccgggc cgtagaaccc gctgaggtag tgcacgcccg gcttcgtgaa caacggatag
       61 agatcgagca gcagcgcgcc gaacacctcg agctgctccc agtcgtcgcg cgcactcagc
      121 gcgtggagga agtcgcccgg aatgccgggg ccgagcggga cacccaagga atcgcgcggc
      181 cgaacgagag cggccgcctc ttcgacgctc agttcccgtg cggtcatccg gggtaggcgg
      241 ccttggcttc gatgatggcg cgctccgcag cctctgcgcc ttcccagccc cgcgtctcgg
      301 tttccttgcc cggttcgatg cgcttgtaga actcgaagaa gtgctcgatc tcggcgatga
      361 cgtgcgggtc gatgtcgccg atgtcttgca cggtgtccca gcggtcgtcg tgcgcgggca
      421 cgcacaacac cttggcgtcg tcgcccttct cgtccgacat ccagaacacc gcgacgggcc
      481 gcgacatcac gtggcagccc gcaaacgtcg gctccggcaa caagacaagt gcgtcgagcg
      541 ggtcgccgtc gtcggcgagc gtgtgcggga agaacccata gtcggccgga tactgcatcg
      601 aggtgaacaa cgtgcggtcg agccagatcg cgccggagtc gtgatccatt tcgtatttgt
      661 tctgcgatcc ccgggggatc tcgacgatga cctcgtgctc ccctgtcatt cgggcggctg
      721 gccttgggcc ggcggccggg gcccgacgac ggtgttgacc gtgcgggtcg cgccgttgac
      781 gcgttcgagt accgcggtca gcagaccgag cgctttggcg ctcgcttcga ggttctggcg
      841 caccgccttt gtttgggtct cgccgtattc cgcgacggcg gcgaccgaat cgaccggtcc
      901 gcgaacgagg cgttcgatct ccttggcgcc cggcaccttg cgcgcgacgt cgatggcgcc
      961 gctcgccacc gcggcggcgg tacgcgacac ctcgccgagc acggtggttt cttcttcggc
     1021 cgaagccagc gcagtcatca catccttctg cgcctggttc gcgaggccga gcgcgtcgcg
     1081 caccaagccc gtgatcgacg ggtcttcggc agccttcacc aaggaacgca tctgccgcag
     1141 cgcttctcga acgacttctt gctcgtcggc gtagcgcccc gatcgcactt gggaccggac
     1201 gaactcctcg agttggggcg gcagcgtgag ttccatcgga ggacctttag atcgtgagca
     1261 ccgtgcgcag cccgcgcccg ttggtcatgt cgtcgaacgc gtcgttgatg tcggccagcg
     1321 ggcggcgctg cgtgatcatt ccgtcgaggt cgagccggcc ggcggcgtag agcgcgagca
     1381 ggcgcggaat gtcgcgccgt ccgttacaac taccgagcag gcagcccatg atcttgcgtt
     1441 ctgtcagcgt gaacatgacc ggcggtgcca acgtgacggt ctggtcgatg ccgccggcgc
     1501 cgaccatgac ggtggtaccg ccgttgcggg tcgaccacag gcaggactcg atgacgggcc
     1561 cggcccctac cgcgtcgaac gcgtagtcga cgccgatgcc gctcgtgagc tgttgcacgg
     1621 tgctgatcac gtcggtggtt gtcgggtcga tgacgtcggt tgcaccgaag ttcttcgcgg
     1681 tcgcgcgtcg ttcggccacg ggatccgaca cgatgatctt cgccgcgcct gcgatgcgcg
     1741 cgccctggac gatcgcgacg ccgatgccgc cgaggccggc gacgaacacg gtcgcgcctt
     1801 ccggaacctt cgcggtgttc agcacggcac cgaccccggt ctgcattgcg cacccgatga
     1861 cgcacgcaac ttcgagcggc gtgtcgtcgt cgaccttgac cgcgccgctc tcgggggtaa
     1921 tgacgtattc cgcgaacccg cccaggccca cgccgcgcat gacgggttga ccgtggcgcg
     1981 acagcggcgt gctgccgtcg agcatcgcgc cggtcgtgag cgagatggcg ttgacgcagc
     2041 acccgtactc gcctcgcagg caccagtaac actcgttgca cgacggcacc ggggtcagca
     2101 cgaccttgtc gcccgcccgc acggaggtca cgccggtgcc aaccttctcg acgatgccgg
     2161 cggcttcgtg gccgagcacc gtcggctgcg tgaacgggaa ggtgccgttc tgcgccgaca
     2221 ggtcggagtg gcacacaccg cagtgcgtca cccgcaccat cacttcgccg gcgcgcggtt
     2281 cctgaatgtc gatgtcgtcg acgatcgcga gtggcttttc ggaggcttcg agcaaggcag
     2341 cacgcatgac cgcacggtaa cgcgtgggct agcgtccgcg cctcgtgagc accgacatcg
     2401 tcgacctgtg ggtcaatctc gtcaccgagc agagcgctga ggacttcacg agccaagagg
     2461 gctacgaaaa catccccggc tacctcggca gcaacaccgg accggtcggg gtgccgtcgc
     2521 tcatcgcact gatggacgag ctcggcgtcg cgaccggcat tttcacaacc ggcatcgacc
     2581 gcagcaccga gaagatgctc gagatttgcg acgcgaaccc cggccgcttt ctggtggcgg
     2641 ccggcgtggt cgacccgtcg cgcccgggcc gggcggtgaa gcgcatccgg gaactcgcgc
     2701 agcacgagcg gctggcgatg attcgcatca tgccgctcgc gtcacagatc ccgacgaacg
     2761 acgctcggca ctaccccatc taccaaatct gcgaggagct cgggatcccg gtcgcaatca
     2821 acgccggcat tcccgggccg cgggtgcgtt ccaaagtgca gcacccgg
//
LOCUS       DASUGA010000776         2757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_88647, whole genome shotgun sequence.
ACCESSION   DASUGA010000776 DASUGA010000000
VERSION     DASUGA010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88647"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VFX21_15245"
     CDS             complement(<1..501)
                     /locus_tag="VFX21_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020114459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEX5097382.1"
                     /translation="MKVVLGVSGGIAAYKAIEVCRRLVDAGVHVAPILTDDSQRFVGA
                     VTFSALASEPAHTSLFAGPDPIPHTTLGQSADLIVVAPATAKLLGKYAAGISDDLLTA
                     TLLATRAPVLLCPAMHSEMWEHPAVQENLATLRERGVHVVDPAEGRLAGGDVGTGRLA
                     DPADIVR"
     gene            complement(517..837)
                     /gene="rpoZ"
                     /locus_tag="VFX21_15250"
     CDS             complement(517..837)
                     /gene="rpoZ"
                     /locus_tag="VFX21_15250"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX5097383.1"
                     /translation="MADRRASLMEPRIESLLPQVDSKFTLVTLAAMRAREINDYYNQL
                     GEGLGRIVPPQVTSVSRKPLSIAMEEVAAGKILFERIEPTEGDVEADADGAPDAGDEP
                     DETA"
     gene            complement(872..1471)
                     /gene="gmk"
                     /locus_tag="VFX21_15255"
     CDS             complement(872..1471)
                     /gene="gmk"
                     /locus_tag="VFX21_15255"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017326484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX5097384.1"
                     /translation="MSPGASHAPTGRRGVLLVLAGTSGAGKGTIGEQLRATHPELRWS
                     VSWTTRPPREGEVEGVDYHFVTRAAFEALRDAGGFLEWFEVYGDLKGTPQAFVDAELA
                     AGHDVMLEVDVQGALAVRAKMPEALLVFVRAPSREEQRRRLLARGSETDESIERRLGQ
                     AAAEEELAERDFDAVVVNDTVEQAVREVAAILDLRRRTG"
     gene            complement(1468..1797)
                     /gene="mihF"
                     /locus_tag="VFX21_15260"
     CDS             complement(1468..1797)
                     /gene="mihF"
                     /locus_tag="VFX21_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HEX5097385.1"
                     /translation="MANPPTLTPEQRQAALEKAAAARRQRAEVKDKLKIGSLSLQELF
                     DQADLRTDSGEMLAKLKVVSVLESLPGVGKVRARNIMKELEISESRRLRGLGTNQRKA
                     LLDRFSG"
     gene            complement(1939..2628)
                     /gene="pyrF"
                     /locus_tag="VFX21_15265"
     CDS             complement(1939..2628)
                     /gene="pyrF"
                     /locus_tag="VFX21_15265"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX5097386.1"
                     /translation="MTDVRDRLALALDMPDVDDAERLAKEVSSWFGIAKVGLELFSAG
                     GHDAIRRLRDLGFVVFADLKFHDIPTTVQRAARVIGGLGVGYLNFHAAGGVDMLRAGV
                     EGLHDGARSAGFRLPVPIAVTVLTSDRDASAFDERLRAAIDGGCGGVVCSVQEIAAVK
                     RSRPEFKTIVPGVRFADDDRHDQSRIGTPAQVAAAGADILVVGRAVTAAVDPRVAAAE
                     VHAHVEATTRV"
BASE COUNT          417 a          965 c          954 g          421 t
ORIGIN      
        1 ccgcacgatg tcggccgggt cggcgaggcg accggtgccg acgtcaccac cggcgagacg
       61 gccctcggcc gggtcgacga cgtggacgcc ccgctcccgc agcgtcgcga ggttctcctg
      121 caccgcgggg tgctcccaca tctcggagtg catggccggg cacaggagca ccggcgcccg
      181 cgtcgcgagc agggtcgcgg tgagcaggtc gtcggagatg ccggccgcgt acttgccgag
      241 cagcttggcg gtcgcgggcg ccaccacgat caggtcggcg gactggccga gtgtcgtgtg
      301 cgggatcgga tcgggcccgg cgaacagcga ggtgtgcgcg ggctcggagg cgagcgcgga
      361 gaacgtcacc gcgccgacga agcgctggga gtcgtcggtg aggatcgggg ccacgtgcac
      421 cccggcgtcg accagacggc ggcagacctc gatcgccttg taggcggcga tcccgcccga
      481 gacgccgagg acgaccttca cgggttggcc ctcgggctac gcggtttcgt cgggctcgtc
      541 gcccgcatcg ggggcgccgt cggcgtcggc ctcgacgtcg ccttcggtcg gctcgatgcg
      601 ctcgaagagg atcttgcccg ccgcgacttc ctccatcgcg atggagaggg gcttgcggga
      661 caccgacgtg acctggggcg gcacgatgcg gccgagacct tcgccgagct ggttgtagta
      721 gtcgttgatc tcccgggccc gcatggcggc cagcgtcacg agcgtgaact tggagtcgac
      781 ctgcgggagc agcgactcga tccgcggttc catcagcgac gctcggcgat cggccatgac
      841 gggagactcc tgagggggag gaagatgccg ctcagccggt ccggcggcga aggtcaagga
      901 tagcagcgac ctcgcgaaca gcctgctcca cggtgtcgtt gacgaccacg gcgtcgaagt
      961 cccgctcggc cagctcctcc tcggccgcgg cctgaccgag gcgccgctcg atcgactcgt
     1021 cggtctcgga gccgcgggcc aggagccgcc gccgctgctc ctcgcgcgac ggcgcccgca
     1081 cgaacaccag cagcgcctcg ggcatcttcg cccggacggc gagcgcgccc tgcacgtcga
     1141 cttcgagcat cacgtcgtgg ccggcggcca gctcggcgtc gacgaacgcc tgcggggtgc
     1201 ccttgaggtc gccgtacacc tcgaaccact cgaggaagcc gcccgcgtcg cgcagcgcct
     1261 cgaacgccgc acgcgtcacg aagtggtaat cgacgccttc gacttcaccc tcgcgagggg
     1321 ggcgcgtcgt ccacgacacc gaccagcgca gttcggggtg cgtcgcccgc agttgctcgc
     1381 cgatcgttcc cttcccggct ccggaggtgc cggcgaggac gagcaggacg ccgcggcggc
     1441 cggtcggcgc gtgcgacgcg cccgggctca cccgctgaag cggtcgagca gcgccttgcg
     1501 ctggttcgtg ccgaggccgc gcagacgacg gctctcggag atctcgagct ctttcatgat
     1561 gttgcgggcg cgcaccttgc cgacgccggg caacgattcg aggacgctga cgaccttgag
     1621 cttcgcgagc atctcaccgc tgtcggtgcg gaggtcggct tggtcgaaca actcctgaag
     1681 tgaaagcgat ccgattttca gcttgtcctt cacctctgcc cgctggcggc gcgcggccgc
     1741 ggctttttcg agcgcggcct ggcgctgctc cggcgtcagc gtgggcgggt tcgccatggc
     1801 agttcctccg atgttgagag ctcagagaag ggaaaaattc tcttgcatcg gtcgcactac
     1861 cgatgcgcag cgggtcagca tagcgatcgc tgcgtttgtc gcaactggag gcgggcttgc
     1921 acgcgctccc gttgcgcgct acacgcgcgt tgtcgcctca acgtgcgcgt gcacttcggc
     1981 tgccgcgacg cgaggatcca cagccgcggt gaccgcgcgc ccgacgacga ggatgtcggc
     2041 gcccgcggcc gcgacttgcg cgggtgtgcc tatgcgtgat tgatcatgtc tgtcatcgtc
     2101 cgcgaagcgc acgccgggca caatcgtttt gaattcgggt cgcgagcgtt tcactgctgc
     2161 gatctcctgc accgagcaca cgacgccacc gcaaccaccg tcgatcgcag ctcgcaagcg
     2221 ctcgtcgaac gcactcgcgt cgcgatcact ggtgagcacc gtcaccgcga tcggaaccgg
     2281 caaccgaaaa cccgcactgc gcgcgccatc gtggagacct tcgacaccgg cgcgcaacat
     2341 gtcgacgcca cccgccgcgt ggaagttcaa gtagccgaca ccgagaccgc cgatgacgcg
     2401 cgcggcccgc tgcaccgtgg tcggtatgtc gtggaacttc aagtccgcga agacgacgaa
     2461 ccccaggtca cgcagccggc gaatggcatc gtgaccgccc gccgagaaca gctcgagccc
     2521 gaccttcgcg atcccgaacc acgacgacac ttccttggcc agccgttcgg cgtcgtcgac
     2581 gtcgggcatg tcgagcgcga gcgcgagccg gtcacgaacg tcggtcactg cattcctccg
     2641 gtcaggtcac gcacgcgcaa cacgtcgtgc gcggcgcacc acttggccag ttcccgtgcg
     2701 atgcgaatcg tcgcccgcgg ctcggcgaac gtcgcggtgc cgacgccgac cgcgtgc
//
LOCUS       DASUGA010000777         2254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_525175, whole genome shotgun sequence.
ACCESSION   DASUGA010000777 DASUGA010000000
VERSION     DASUGA010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525175"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VFX21_15270"
     CDS             <1..309
                     /locus_tag="VFX21_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097387.1"
                     /translation="WAQLIEMRLKAGSDTAELTKEIRAAEQSGSGLIRSLIMHDQSDP
                     NCVYTLVMFESEEKARAREQDPRRQEQLQRARTIMGEIFDGPPAFTDLIVDDEWTDAG
                     "
     gene            327..731
                     /locus_tag="VFX21_15275"
     CDS             327..731
                     /locus_tag="VFX21_15275"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX5097388.1"
                     /translation="MDNATTMRATYERINAGDIDGFGELLANDFVEHEGTPGLPPTKS
                     GTLDFFRMLSAAFPDMHMAVDDLIADEDKTVARVTVKATHKGEFMGVPATGNPVSMQL
                     IDIMRFDRAGLVCEHWGVADMLGLMQQLGTIS"
     gene            869..>2254
                     /locus_tag="VFX21_15280"
     CDS             869..>2254
                     /locus_tag="VFX21_15280"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5097389.1"
                     /translation="MTPLVGRSDELDQMRRWLAEALAGSPRIVVMTGEAGIGKSRLVN
                     ELISDARTANVRPFAGRCLEGSRVAFLPFTTLVDTLLPDHWSAEELIARLSRSLMTSA
                     ADRALLVVVEDAQWADDPSMELLAHIAATFAHEAVFRQLPVMLLVTARSSGVPPTVQR
                     LLSRLGREVIGRTVRLGALDDLAVFAMLEACTGVSPSVQLLTHIQETTAGNPHEVEAL
                     LSRLEHAGALQVRGRELIATVEDYSTLPGDGDAAPLIAELSDAARLLLTTLALLRDGR
                     VTSVRIASGLATIDFDAGLDEAIDAGLLTDDGVRLEFVHAATRHALVAGLGGRRRHRS
                     HADIARRLADAPPDLHISATEIVDQLHRARDQADSLLLTRFARVAGDECLALGAHAEA
                     LRHYEAALSLPGLRDDMRAALEERAALAAYRNTDRSSATAHASRAIELAQRQGDLDTW
                     GEAALLLARASS"
BASE COUNT          391 a          766 c          711 g          386 t
ORIGIN      
        1 tgggcgcagc tcatcgagat gcgattgaag gcgggatcgg acaccgcgga gctgacgaag
       61 gagattcggg ccgctgagca atccggatca ggtctcattc gctcactcat catgcacgac
      121 cagtccgatc ccaactgcgt ctacacgctt gtgatgttcg aaagcgaaga aaaggcacgc
      181 gctcgtgaac aggacccacg tcgccaagag caacttcaaa gggcccgcac gatcatgggc
      241 gagatcttcg acggtccgcc tgcgttcacc gacctcatcg tcgacgacga gtggacggac
      301 gcagggtgaa ccagcaagga gcgagcatgg acaacgcaac gacaatgcgc gccacgtacg
      361 agcggatcaa cgccggtgac atcgacggct tcggcgagtt gctcgccaac gacttcgtcg
      421 agcacgaagg cacacccggg cttccgccga cgaagtccgg gacgctcgac ttcttccgca
      481 tgttgtccgc ggcgttcccc gacatgcaca tggccgtcga cgacctgatc gcggacgagg
      541 acaagaccgt ggcacgcgtc accgtcaagg caacacacaa gggcgagttc atgggagtcc
      601 ccgcgaccgg aaatcccgtg tcgatgcagc tcatcgacat catgcgcttc gaccgcgccg
      661 ggctggtgtg cgagcactgg ggcgtcgccg acatgctcgg cctgatgcag cagctcggga
      721 caatttcata ggcggccgac tggaaccgct ccggcgcaat ctcagttgcc ctcgttcttg
      781 ctccatgcca gggtttgcac ccggggagaa gcgagttccg ctggtgctgt acgtcatgcg
      841 cggacgctga gtcgtgaacg accgagctgt gactccgctc gtcggtcggt cggacgagct
      901 cgaccagatg cgccggtggc tcgcggaggc actcgccggc agcccgcgca tcgtcgtcat
      961 gaccggtgaa gcggggatag gcaagtcgcg gcttgtgaac gagctgatct cggacgcgcg
     1021 cacggcgaac gttcgcccgt tcgccggccg ttgcctcgag ggttcgcgcg tcgcattcct
     1081 tccgttcacg acgctcgtcg acacgttgct tcccgatcat tggtccgccg aggaactcat
     1141 cgcgaggctc agtcgttcgc tcatgacgag cgcggccgat cgcgcgttgc tcgtcgtcgt
     1201 cgaagacgcg cagtgggccg acgacccgag catggaactt ctcgcgcaca ttgcggcgac
     1261 gtttgcgcat gaagccgtct ttcggcagct ccccgtgatg cttctggtga ccgcacgatc
     1321 gagtggagtt ccgcccactg ttcaacgact cttgtcgcgg ttggggcgcg aagtcatcgg
     1381 ccggacagtt cgcctcgggg cgctcgacga cctcgccgtc ttcgcgatgc tcgaggcctg
     1441 taccggcgtc tcgccgtcag tgcagcttct gacgcacatc caggagacaa cggccggcaa
     1501 cccgcacgag gtcgaggctc tcctctcgcg cctggagcac gccggcgcat tgcaggtacg
     1561 cggccgtgaa ctcatcgcca ccgtcgagga ctattcgacc ttgcccggcg atggcgatgc
     1621 cgcaccgttg atcgcggagc tctcggacgc ggcacgactg ttgctcacga ccctcgcgct
     1681 cctgcgcgac ggccgcgtca cctcggtacg tatcgcgagc gggctcgcga ccatcgactt
     1741 cgacgcgggc ctcgacgaag cgatcgatgc cggccttctc accgacgacg gtgtacgtct
     1801 cgaattcgtg cacgcggcga cacggcacgc gctcgtcgcc ggtctcggcg gacgacggcg
     1861 acatcggagt catgccgata tcgcacggcg cctcgccgac gcaccgcccg atttgcacat
     1921 cagcgccacc gagatcgtcg accaactcca tcgcgcccgg gaccaagccg actcgttgct
     1981 gctgacccgt ttcgcgcgcg tcgccggcga cgaatgcctc gcgctcggcg cacacgccga
     2041 ggcgctccgt cactacgagg ctgcgttgag ccttcccggc ctgcgcgacg acatgcgcgc
     2101 ggcgctcgaa gagcgcgccg cgcttgccgc ttatcggaac accgatcgtt cttccgcgac
     2161 cgcgcatgca agtcgcgcca tcgaactcgc ccagcgacaa ggtgatctcg acacgtgggg
     2221 agaggctgcg ctgcttctcg cccgcgcttc gtca
//
LOCUS       DASUGA010000778         2895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1619510, whole genome shotgun sequence.
ACCESSION   DASUGA010000778 DASUGA010000000
VERSION     DASUGA010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619510"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..1089)
                     /locus_tag="VFX21_15285"
     CDS             complement(265..1089)
                     /locus_tag="VFX21_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5097390.1"
                     /translation="MTAIAVPLPVDRPHRSRFNWAVADAMTVTWRNIIAMTRTPQVLV
                     FSTIQPIIFVILFRYVFGGAIQIPGVPSSQYVDYLMPGVFAQTVVFGSVQTGVALAED
                     MNKGIIERFKALPMSRSSVLAGRTIADLVRNVFVVGLMCIIGVIVGWRVHTNPLDAVL
                     AIALMLFFSHALSWIFAIVGLAAQNAEAAQAAAFPILAPLVFASTAFVSAETMPGPLK
                     WFAEHQPVSITVDAVRSLTYGGIFHDPSKVVASVIWSVAIIAVTAPLAVRMYRRKV"
     gene            complement(1086..2081)
                     /locus_tag="VFX21_15290"
     CDS             complement(1086..2081)
                     /locus_tag="VFX21_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003954882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5097391.1"
                     /translation="MTVNASPHAIRVEGLRKNFGDVLALQGVDFSVPAGTVLGLLGPN
                     GAGKTTAVRVLTTILQADGGVAEVLGVDVSRDPQAVRERIGLAGQYAAVDENLTGHEN
                     LRMVGRLTHMAKPAIRARADELLERFDLVDAANRPVRTYSGGMRRRLDLAAALVHKPP
                     VLFLDEPTTGLDPRSRTDLWGVIEELVADGTTLLLTTQYLEEADRLADNIVVIDHGTV
                     IAEGTSGELKARLGATIIEVGFVDHAAAEQAQAVLCQIGLCDVETASHTVALRTDAGT
                     KTVLEVVRTLDQSKLVPETLNIREPTLDDVFLELTGHAAEPPPDDADDEISRGAA"
     gene            2182..2634
                     /locus_tag="VFX21_15295"
     CDS             2182..2634
                     /locus_tag="VFX21_15295"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5097392.1"
                     /translation="MAANLPDSGLDLASDVWRMMTELAVERKSSFPAVAASHGLNPGA
                     LHALLSLEEGEPKSMSALAVAWRCDASNVTWLVDRLEEKGLAERRAHPTDRRVRTVAL
                     TRKGAKTRAKVMALLHEAPDSIRKLSPTDLATLHRILAKVVPSNAAAS"
     gene            2634..>2895
                     /locus_tag="VFX21_15300"
     CDS             2634..>2895
                     /locus_tag="VFX21_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097393.1"
                     /translation="MPRFFTEHEYAVVDALADMVIAPGDGHPGGRALGVVDFVDNLLG
                     AFTVDPPLIYAGGPFSGRAGGDASFGNFFPLSRNEELAWRTRI"
BASE COUNT          496 a          922 c          973 g          504 t
ORIGIN      
        1 ctggtttcgt cggtgatgcg agcctcgatg gtgacggctg cggcgccgtc ggccgtgtcg
       61 atggtcgcgg gcgtgatcga gagcgacgcg agcacgggcg cgtcgaagtc agttgccgca
      121 ccgctggtcc ccgacccgac ggcaccgagg accggtaccg tcgcagccag gactgcagcc
      181 cctacgacga atcgtcgagt ccgccccgcc acaccgtttc gatcggcggg ccgcggcgaa
      241 ttccaaggac agccaggtca ggaatcagac cttccggcgg tacatccgga ccgccagggg
      301 ggccgtcacc gcgatgatcg ccacgctcca gatcacggac gccaccacct tgctggggtc
      361 atggaagatg cccccgtagg tgagggatcg gacggcgtca accgtgatcg acaccggctg
      421 gtgctcggcg aaccacttga ggggcccggg catcgtttcg gccgacacga atgccgtcga
      481 cgcgaacacg agcggagcga ggatcggaaa cgctgcggcc tgggcagctt ccgcgttctg
      541 cgcggccagt ccgacgatgg cgaagatcca cgagagtgcg tgtgagaaga agagcatcag
      601 cgcgattgcg agtaccgcgt cgagcggatt ggtgtgaact cgccagccga cgatgacacc
      661 gatgatgcac atcagcccga cgacgaacac gttacggacc aagtcggcga tcgttcgccc
      721 ggcgaggacg gatgagcgcg acatcggaag cgccttgaac cgttcgatga tgcccttgtt
      781 catgtcctcg gcgagcgcga ctccggtttg caccgaaccg aacacgacgg tttgcgcgaa
      841 gacgccgggc atgaggtagt cgacgtactg cgacgagggc acgccgggaa tctggatcgc
      901 gccgccgaac acgtatcgga acaggatcac gaagatgatg ggctgaatcg tcgagaagac
      961 gagcacctgc ggcgtacgcg tcatcgcgat gatgttgcgc cacgtcacgg tcatcgcgtc
     1021 ggcgaccgcc cagttgaacc ggctgcggtg cggccggtcg acgggaaggg gaactgcgat
     1081 cgccgtcatg ctgcacctcg cgagatttcg tcgtcggcgt cgtcgggggg cggttctgcc
     1141 gcgtggcctg tgagctccag aaacacgtcg tcgagcgtgg gctcgcgaat gttcaacgtt
     1201 tcgggcacga gcttcgattg gtcgagcgtg cgcacgacct cgagcaccgt cttggtgccg
     1261 gcgtcggtgc gtaaggccac ggtgtgcgag gcggtctcta cgtcgcacag cccgatctgg
     1321 cacaacaccg cctgcgcctg ctcggcggcg gcatggtcga cgaagcccac ctcgatgatc
     1381 gttgcaccga gccgcgcctt gagctcgccc gacgttccct ccgcgatcac ggtgccgtga
     1441 tcaatgacca cgatgttgtc ggccaagcgg tccgcttcct cgagatactg cgtcgtcagc
     1501 agcagcgtgg tgccgtcggc gacaagctct tcgatgacgc cccacaagtc ggtgcggctg
     1561 cgcgggtcga gtccggtagt cggttcgtcg agaaacagga ccggcggttt gtgcacgagc
     1621 gcggccgcga ggtcgaggcg gcggcgcatg ccgccgctgt aggtgcgcac cgggcgattg
     1681 gcagcgtcga caaggtcgaa acgttcgagc agctcgtcgg cacgcgctcg gatggccggc
     1741 ttcgccatgt gtgtgaggcg gccaaccatg cgcaagtttt cgtggccggt caggttctcg
     1801 tcgacggcgg cgtactggcc tgcgagacca atgcgttcgc gcacggcttg cgggtcacgc
     1861 gagacgtcga cgccgagcac ttcggcgact cccccgtcgg cttggagaat cgtggtcaac
     1921 acgcgaaccg ccgtggtctt gcccgcacca ttgggaccga gcagtccgag cacggttccg
     1981 gccggtaccg agaagtcgac gccttggagt gcgagcacgt cgccgaagtt cttgcgcagc
     2041 ccttcgacgc gaatcgcatg gggggatgcg ttcacggtca tagcttgcac cctacgccgg
     2101 atccttgaat gcggtcaagt atttagtttc ttcaatattg tgatgcttga agtatttcgc
     2161 gaattggctt acgctggcgt ggtggctgcg aatcttcccg attccggttt ggatctcgca
     2221 tccgacgtct ggcggatgat gactgagctt gcggtggagc ggaagtccag ttttcccgcg
     2281 gtcgccgcga gtcacgggct gaatcccggg gcgctccacg cgcttctctc gctcgaggag
     2341 ggtgaaccga agtcgatgag cgcgcttgcg gtcgcgtggc gttgcgacgc gtcgaacgtc
     2401 acgtggctcg tcgaccggct cgaagaaaaa ggactcgccg aacggcgcgc gcaccccacc
     2461 gaccggcgag tccgcaccgt cgcgctcacg cgcaagggtg cgaagacgcg cgccaaggta
     2521 atggcgctgc tgcacgaagc gccggactcg atccgcaagc tctctcccac cgacctcgcg
     2581 acgttgcacc gaattctcgc caaagtcgtt ccatccaacg ccgccgcgtc gtagtgccgc
     2641 gcttcttcac ggaacacgaa tacgccgttg tcgacgcgct agccgacatg gtcatcgcgc
     2701 ccggtgacgg ccatccgggc ggccgggccc ttggcgtcgt cgacttcgtc gacaacctgc
     2761 tcggcgcgtt caccgtcgat ccgccgctga tctacgccgg cggcccgttc agcggccgcg
     2821 cgggcggtga cgcgtcattc ggcaacttct tcccgctctc gcgtaacgaa gaacttgcgt
     2881 ggcgaacccg catcg
//
LOCUS       DASUGA010000779         4737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1062927, whole genome shotgun sequence.
ACCESSION   DASUGA010000779 DASUGA010000000
VERSION     DASUGA010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062927"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VFX21_15305"
     CDS             complement(<1..302)
                     /locus_tag="VFX21_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097394.1"
                     /translation="MLLVGTEKQLLDLDADHVIAEVGVTALAPPFVLADRQRVLHVDG
                     TEVGALPTTDAQSIAPLGDEQALVGRTDARLAVVSSADVRALSSFEDVPGRDDWQNP"
     gene            518..1918
                     /gene="xylB"
                     /locus_tag="VFX21_15310"
     CDS             518..1918
                     /gene="xylB"
                     /locus_tag="VFX21_15310"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019609054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEX5097395.1"
                     /translation="MPLVLGVDSSTQSTKVEVRDADTGALIAEGRAPHPPTSPPRSEQ
                     HPDVWWDALLGAFAGAGGGTVDALAVGAQQHGLVALDASGAVLRPAKLWNDTESASDA
                     QWLLSQADATVWADACGSVPVAAFTVTKLSWLHRSEPEAWERLARVCLPHDYLTLRLT
                     GEFVTDRGDASGTGYYSAAEGRYRDDLLAIVERDVDWSRRLPRVLAPTEAAGRAERFR
                     NAVVAPGTGDNMAAALGVGARPGDVVVSLGTSGTVYAVSDTPTHDASGSVAGFADASG
                     RYLPLVCTLNATKVTDAIARVLGVDHPTLDDLALAAAPGAGGVVLVPYFDGERTPNRP
                     DARGMLAGLRPDVTREQIARAAVEGVICGLLDGLDALLACGIEADGRCILIGGGARSR
                     AAARVLADLSGREVVTPDAAEHVAMGACVQAAAVLGGHPIDAVLDQWRLGRGTVIEPE
                     PIDRDAVRDRYRAARG"
     gene            1928..3304
                     /locus_tag="VFX21_15315"
     CDS             1928..3304
                     /locus_tag="VFX21_15315"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HEX5097396.1"
                     /translation="MDVADSIVDLIGHTPLVRLRRLVEAESLHCDLLAKVELVNPGGS
                     VKDRIAVAMIDEAERTGALRPGGTIVEPTSGNTGAGLAIVAAQRGSKCIFVMSDKMSD
                     EKVALLRSYGAEVVVCPTAVPPEDPRSYYSTAERLVRETPGAFRPDQYSNMANPRAHE
                     LSTGPEIWQQTEGTVTHFVAGIGTGGTITGVARYLKSQNPDVQIVGADPAGSVYSGGS
                     GRPYLVEGVGEDFWPTTFDPTLVDRVVEVSDADSFLTARRVTRDEGLLIGGSCGTAVH
                     AALEVARELHHGDVVVVLLPDSGRSYLSKIFDDGWMYDMGFLRAAGMVAGDVLDTKGN
                     RMPDLVLITPEEPVRDAIKLMHDTGVSQLVVSVTKELPLAVKEVTGSLQELALMDLAF
                     RDPAVLDRPVGDVCGAPMPMVGVGEPIARVVECLEQGPSIVVVGDGHPIGVLTRSDVL
                     SFLAARTS"
     gene            3307..4398
                     /locus_tag="VFX21_15320"
     CDS             3307..4398
                     /locus_tag="VFX21_15320"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEX5097397.1"
                     /translation="MADGFETRAIHAGQAPDPATGAVVPPISLATTFAQASVGEHQGY
                     EYSRSGNPTRTALEACLASLEDATHGLAFASGLAAEDAILRALAPADHVVVPHDAYGG
                     TYRLVARVHEPHGLEFTPADLSDPAALAAAWRPNTKLVWIESPTNPMLATVDIAAVSR
                     FAHEHEAIVVVDNTFATPYLQRPLTLGADVVVHSSTKYLGGHSDVVGGFVATNDDALG
                     ERLRFLQNAMGAVPSPFDCYLVLRGVKTLAVRMDRHQANAQAIVALLESHPAVAKVLY
                     PGFGGMVSFIAAGGEGAALEIVARTRVFTLAESLGAVESLIEHPARMTHASVADSPLA
                     VDPALVRLSVGLETLDDLLADLRGALDRS"
     gene            4395..>4737
                     /locus_tag="VFX21_15325"
     CDS             4395..>4737
                     /locus_tag="VFX21_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation protein TolB"
                     /protein_id="HEX5097398.1"
                     /translation="MNRRDFLRMGAVTPLVMVAIPTTDSPYGDLNPPDANGLMLPNGF
                     RSRVVGTSGEVVTGTNYTWHVFPDGGATFAMPDGGWVYASNSEIPGGTAGGVGALRFD
                     AGGQIVDAYPIC"
BASE COUNT          672 a         1657 c         1682 g          726 t
ORIGIN      
        1 gggttctgcc aatcgtcgcg accggggacg tcttcgaacg aggacaaggc gcgaacgtcc
       61 gccgacgaca cgacggcgag tcgggcgtcg gtacggccga cgagtgcctg ttcgtcgccg
      121 agcggcgcga tgctctgcgc gtcggtcgtg gggagtgcac cgacctcggt gccgtcgacg
      181 tgcagcacgc gttgccggtc ggcgaggacg aacggtggcg cgagcgcggt caccccgacc
      241 tccgcgatca catggtcggc gtcgaggtcg aggagctgtt tctccgtgcc gacgaggagc
      301 atgacgtcga gggtaggcgt gcggacggag cgaagccacg gacggcgcag cgagggagcc
      361 attgtcatgc ctcgtggcgc ggcccgtcgt tcgctaggtt cggtttgtgg cggattggat
      421 cccgcccgag tccgacttcg acgccgaccg tttctggcag ccgcgcgagt accagtgccc
      481 tgaccggtgt gcggggatgc accctaaggt cggcaccatg ccgctggtcc tcggcgtcga
      541 ctcgtcgaca cagtccacca aggtcgaagt gcgcgatgcc gacaccggtg cgctgatcgc
      601 cgaaggccgg gcgccgcatc cgccgacctc accgccgcgc agtgagcagc acccggacgt
      661 gtggtgggac gcgctgctcg gcgcattcgc cggtgcgggc ggcggcaccg tcgacgcgct
      721 cgcggtcggc gcgcaacaac acgggctcgt cgcgctcgac gcgtccggcg cggtgctccg
      781 gcccgcgaaa ctctggaacg acacggagtc ggcgtccgat gcgcagtggc tgctctcgca
      841 ggccgacgcg accgtgtggg cggacgcgtg cggatcggtg ccggtcgccg cgttcaccgt
      901 cacgaagctc tcgtggttac accgctccga gcccgaggca tgggaacggc tcgcgcgcgt
      961 ctgccttcca catgactatt tgacgttgcg gctcaccggt gagttcgtca ccgaccgcgg
     1021 cgacgcgtcg gggacgggct actactcggc cgcggagggc cgctatcgcg acgacctgct
     1081 ggcgatcgtc gagcgcgacg tcgactggtc acgccgcctg ccgcgcgtgc tcgcgccgac
     1141 ggaggctgcg ggccgcgccg aacgattccg caacgcggtg gtcgcgcccg gtaccggcga
     1201 caacatggcc gccgcgctcg gtgtcggtgc gcgtccgggg gatgtcgtcg tctcgctcgg
     1261 cacttcgggc acggtgtacg cggtcagcga cacgccgacg catgatgcgt cgggatcggt
     1321 cgccggcttc gccgacgcct cgggccgtta tctcccgctc gtgtgcacgc tcaacgcgac
     1381 gaaggtcacg gacgcgatcg cgcgtgttct cggtgtcgac caccccacgc tcgacgacct
     1441 cgcgctcgcg gccgcaccgg gcgcgggtgg cgtggtgctg gtgccgtact tcgacggcga
     1501 acgcacgccg aaccggcccg acgcgcgcgg catgctcgcc ggactgcgcc cggacgtcac
     1561 gcgcgagcag atcgcgcgtg ccgccgtcga gggcgtcatc tgcggtctgc tcgacggact
     1621 cgacgcgctg ctcgcgtgcg ggatcgaagc cgacggccgg tgcatcttga tcgggggtgg
     1681 cgcgcggtca cgcgccgcgg cccgcgtcct cgccgacctg tcgggccgcg aggtcgtgac
     1741 ccccgacgcg gccgagcacg tcgccatggg cgcatgcgtg caggccgccg cggtgctcgg
     1801 cggccacccg atcgacgcgg tactcgacca atggcgcctc ggtcgcggca ccgtgatcga
     1861 gcccgaaccg attgatcgcg acgcagtacg ggatcggtac cgcgccgcgc gagggtaggt
     1921 tcacggggtg gacgtcgccg actcgatcgt cgatctcatc ggtcacacgc cgctcgtgcg
     1981 gttgcggcgc ctggtcgagg ccgaaagcct gcactgcgac ctgctggcca aggtcgagtt
     2041 ggtgaatccg ggtgggagtg tgaaggaccg catcgcggtc gcgatgatcg acgaagccga
     2101 acgcacgggc gcgttgcggc cgggcggcac catcgtcgag ccgacctcgg gcaataccgg
     2161 cgcgggcctc gcgatcgtcg ccgcccaacg cggctccaag tgcatcttcg tcatgagcga
     2221 caagatgagc gacgagaagg tcgcgctgtt gcgctcgtac ggcgccgaag tcgtcgtgtg
     2281 cccgacagcg gtaccacccg aagacccgcg ctcgtactac tcgacggccg agcggctggt
     2341 gcgcgagacg ccgggcgcgt tccggcccga ccagtactcg aacatggcga acccgcgcgc
     2401 gcacgagctg tcgaccggcc cggagatctg gcaacagacc gagggcacgg tcacgcactt
     2461 cgtcgccggc atcggcaccg gtggcaccat cacgggcgtc gcgcgttact tgaagtcgca
     2521 gaaccccgac gtccagatcg tcggcgccga ccccgcgggg tccgtgtact ccggcggatc
     2581 cggccgtccc tacctcgtgg agggcgtggg cgaagacttc tggcccacca cgttcgaccc
     2641 gacgctcgtc gaccgcgtcg tcgaggtcag tgacgccgac tcgttcctca ccgcgcggcg
     2701 tgtcacgcgc gacgaagggc tactgatcgg ggggtcgtgt gggaccgcgg tccacgccgc
     2761 gctcgaagtc gcgcgcgagt tgcaccacgg tgacgtcgtc gtggtgttgc tgcccgactc
     2821 cggccgcagc tacctgtcga agatcttcga cgacgggtgg atgtacgaca tggggttcct
     2881 gcgggccgcg ggcatggtcg ccggcgacgt gctcgacacc aagggcaacc gcatgcccga
     2941 tctcgtgctc atcacgcccg aggaaccggt gcgcgacgcg atcaagctga tgcacgacac
     3001 cggcgtgtcg cagctcgtcg tgagcgtcac caaggaactg ccgctcgcgg tcaaggaagt
     3061 gacgggctcg ctgcaggagc tggcgctgat ggacctcgcg ttccgcgacc cggcggtgct
     3121 cgaccggccc gtcggcgacg tctgcggcgc gccgatgccg atggtcggcg tcggcgaacc
     3181 gattgctcgc gtcgtcgagt gcctcgaaca gggtccgtcg atcgtcgtcg tcggcgacgg
     3241 ccacccgatc ggcgtgctga cgcgctccga cgtcctcagc ttcctcgcgg cccggacttc
     3301 gtagccgtgg ccgacggctt cgagacacgg gcgatccacg cggggcaggc gcccgacccg
     3361 gcgacgggcg cggtcgtgcc gccgatctcg ctcgcgacga cgttcgcgca ggcgagcgtc
     3421 ggggagcacc agggctacga gtactcgcgc agcggcaacc cgacgcgcac tgcgctcgaa
     3481 gcgtgcctcg cgtcgctcga agacgcgacg cacggtctcg cgttcgcgag cggtctggcg
     3541 gccgaggacg cgatcctgcg ggcactggca cccgcggacc acgtcgtcgt gccgcacgac
     3601 gcgtacgggg gcacgtaccg gctcgtcgcg cgcgtgcacg aaccccacgg gttggagttc
     3661 acaccggccg acttgagtga tcccgcggcg ctcgccgccg cgtggcgacc caatacgaag
     3721 ctcgtgtgga tcgagtcgcc gacgaacccg atgctggcaa cggtcgacat cgcggccgtg
     3781 tcacgcttcg cgcacgagca cgaagcaatc gtcgtcgtcg acaacacctt cgcgaccccg
     3841 tacctccagc ggcccctcac gctcggcgcc gacgtcgtcg tgcactcgtc gacgaagtac
     3901 ctcggcggcc actccgatgt ggtgggcggc ttcgtcgcca cgaacgacga cgcgctcggg
     3961 gaacggctcc ggttcctgca gaacgcgatg ggcgcagtgc cgtcgccgtt cgactgctat
     4021 ctcgtgctgc gcggcgtgaa gacgctcgcg gtgcgcatgg accgccacca agcgaatgcg
     4081 caggcgatcg tcgccctgct cgagtcgcat cccgcggtcg ccaaggtcct gtatcccggc
     4141 ttcggcggca tggtctcgtt catcgcggcc ggcggtgaag gtgccgcgct cgagatcgtg
     4201 gcgcgcacgc gcgtgttcac actcgcggag tcgctcggtg cggtcgagtc gttgatcgag
     4261 catccggcgc gcatgacgca tgcatcggtc gcagactcgc cactcgccgt cgaccctgca
     4321 ctcgtgcgtc tttcggtggg gctcgagacg ctcgacgact tgctcgccga cctacgcggc
     4381 gcgctggacc gttcgtgaat cgccgcgact tcctccgcat gggcgcggtc acaccgctcg
     4441 tgatggtcgc gatcccgacg accgacagcc cgtacggcga cctcaacccg cccgatgcca
     4501 acggactcat gctgccgaac ggcttccgct cgcgcgtcgt cgggacgtcc ggcgaggtcg
     4561 tcaccggcac caactacacc tggcatgtgt tccccgacgg tggcgcgacg ttcgcgatgc
     4621 ccgacggcgg atgggtgtac gcgtcgaaca gcgagatccc cggcggcacc gcgggcggtg
     4681 tcggcgcgct tcgcttcgat gccggtggtc agatcgtcga cgcgtacccg atctgcc
//
LOCUS       DASUGA010000780         3247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1620000, whole genome shotgun sequence.
ACCESSION   DASUGA010000780 DASUGA010000000
VERSION     DASUGA010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620000"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..81
                     /locus_tag="VFX21_15330"
     CDS             <1..81
                     /locus_tag="VFX21_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEX5097399.1"
                     /translation="PVGGDLEYADEVTLGRALEGRRDVDA"
     gene            complement(114..857)
                     /locus_tag="VFX21_15335"
     CDS             complement(114..857)
                     /locus_tag="VFX21_15335"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5097400.1"
                     /translation="MSLLLIAAIGLVIGFLGGLLGKGGSAIATPLLHAMGVPAIVAVA
                     APLPATVPSTAVASYAYWRERFVDWRVVRWSIGIGVPCTVFGAVLTRWVGGGALVWAT
                     DLVLVALGLRVLLGASTPSTSVVDTADPSPAKLAAVAGGVGIVSGLLANSGGFLLAPL
                     FITVLRLPIKAALATSLAVACVLAVPGTIVHAALGHIDWTVVAVFAATSVPFSFLGAR
                     IAIRTEAHRVERFYGLALMVLGLTFLAIS"
     gene            957..1823
                     /locus_tag="VFX21_15340"
     CDS             957..1823
                     /locus_tag="VFX21_15340"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5097401.1"
                     /translation="MDLRQLEYLVAIDDCGTFTAAAAKLHVAQPSLSHAIRRLEHELG
                     VELFARLGRSVAPTEAGREVIDSARQVLRDMADVRAAAAAATTLVRGTLDLTALPTLA
                     VDPLAGLIGRFRSEHEGVTIQVHEPETTAEVEATVRSGRAELGFTDITTGGRGLVRIA
                     LSRQDVVVVSPPGRADAATAVTPSMLASIPLVVTPVGTSTRRLLDMTLSRGGYEPDIA
                     VEIGHREAIVALVLAGAGSSLLPRPLAIEAAARGAIVRELKPVLTRRVGILHRRGRLS
                     PAALAMISMARS"
     gene            complement(1835..2212)
                     /locus_tag="VFX21_15345"
     CDS             complement(1835..2212)
                     /locus_tag="VFX21_15345"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HEX5097402.1"
                     /translation="MIPQTQTRSFREFRLLAAGMIAAGAVWPLLPIHPPLLCPLRSAT
                     GIPCPFCGMTRSVVALLHGDVVASLRFNPGGIFIVALAIVLLAGPSWLKAINARYHPS
                     VWLLVPVFVALWAYNIGWNPTFN"
     gene            complement(2209..2946)
                     /locus_tag="VFX21_15350"
     CDS             complement(2209..2946)
                     /locus_tag="VFX21_15350"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX5097403.1"
                     /translation="MDLDNRLTIATPEGVTIDVVIAGLGSRFVARLLDSLIQLAIILA
                     LLFGAGIVATGVSTGVAIAVAIVVIFLVVLAYDIPFEVTGVGTPGKRAMGIRVVARGA
                     EPVDFVTSSVRNLLRIIDFIGFYLVGAVSIVSTQHSQRLGDLAAGTLVIRERFGGRKD
                     KPVPIAPITVPLAQVQGWDVTAIDDEELVTVRHFLDRRLALPWPIRSYFAAELVRRLW
                     PKVPGVPHDAHAEFVLEGIVVAKQARA"
     gene            2947..>3247
                     /locus_tag="VFX21_15355"
     CDS             2947..>3247
                     /locus_tag="VFX21_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097404.1"
                     /translation="METVVAAPAAGNASPVERFVAERAPVWDELEQLVTQAKNRPSRV
                     GAHGVRRLGETYRAAAADLAIARRQYPGSPFIVRLERLVQRARTAVYHSSRSSGTF"
BASE COUNT          551 a         1126 c         1139 g          431 t
ORIGIN      
        1 ccggtcggcg gcgacctcga gtacgccgac gaggtcacac tcggtcgtgc gctcgaaggc
       61 cgccgcgacg tcgatgcctg agcgcgtgta cgccgcgagc cggccgcgcg cactcagctg
      121 atcgcgagga acgtgagacc gagcaccatc aacgccaggc cgtagaagcg ctccacgcga
      181 tgtgcctccg tgcgaatcgc gatccgtgca ccgaggaacg agaacggtac ggaggtcgcc
      241 gcgaagactg cgaccactgt ccaatcgatg tgtccgagcg cggcatggac gatggtgccc
      301 ggcaccgcca gcacgcaggc cacggcgagc gacgtggcga gcgcggcctt gatcgggagc
      361 cgcagcaccg tgatgaacaa cggcgccagc aggaacccac cgctgttggc caggagcccc
      421 gacacgatgc ccacgccccc cgcgaccgca gcgagcttgg ccggagacgg atcggcggtg
      481 tcgacgactg acgtcgacgg cgtactcgcg ccgagcaaga cgcgcaggcc aagggccacg
      541 agcaccaggt cggtcgccca gaccagtgcg ccgccgccca cccatcgcgt caagaccgcg
      601 ccgaacacgg tgcaggggac gccgataccg atgctccagc gcacgacccg ccagtcgacg
      661 aatcgctcgc gccaatacgc gtagctggcg acggccgttg acggaaccgt ggccggcaac
      721 ggtgcggcga cggcgacgat cgcgggcacg cccatcgcgt ggaggagcgg tgtcgcgatt
      781 gccgagccgc ccttgccgag caagcctccg aggaaaccga tgaccaaccc gattgcggcg
      841 atgagcaaga ggctcacggt cgtctcgggg tgcggttcct ggtcaccctc ccatgatcct
      901 cccgcggaac cgatatagct tgcgcatagt tgatatgaga ccgataggga atatctatgg
      961 atctgcgcca gctcgaatat ctcgtcgcga tcgacgactg cgggaccttc accgccgcgg
     1021 ccgcgaagct ccacgtggcg cagccgtcgc tctcgcacgc cattcgccgc ctcgagcacg
     1081 agctcggcgt cgagctgttc gcgcgcctgg gtcgctcggt cgcacccacg gaggccgggc
     1141 gcgaggtcat cgacagcgcc cgccaggtgc tgcgcgacat ggccgacgtc cgcgccgcgg
     1201 ccgccgcggc gacgacgctg gtccgcggga cgctcgatct gaccgcgctg ccgacgctcg
     1261 cggtcgaccc ccttgccggg ctgatcggcc gcttccggag cgagcacgaa ggggtgacga
     1321 tccaggtgca cgaaccggag accacggccg aggtggaggc aaccgtgcgc tccggccggg
     1381 cggagctcgg attcaccgac atcacgaccg gtgggcgcgg tctcgtgcgg atcgcgctct
     1441 cccgtcagga cgtcgtcgtc gtctcgccgc ccggccgcgc cgacgcggcg acggcggtga
     1501 cgccgagcat gctcgcgtcg atcccgctgg tcgtcacgcc ggtcggtacg tcgacgaggc
     1561 ggctgctcga catgacgttg tcgcgcggtg gctacgagcc cgacatcgcg gtcgagatcg
     1621 ggcaccgtga agcaatcgtc gcgctggtgc tcgccggcgc agggtcgtcg ctgctgccgc
     1681 gacctctcgc gatcgaagcc gcggcccggg gtgccatcgt ccgcgagctc aaacccgtgc
     1741 tcactcggcg cgtcggcatc ctccatcggc gcggtcggct ctcacccgcc gcgctggcga
     1801 tgatctcgat ggcgcgcagc tgatcgccga gtgcctaatt gaacgtcggg ttccacccga
     1861 tgttgtacgc ccacaacgcg acgaagacgg gcacgagcag ccacaccgat gggtggtagc
     1921 gcgcgttgat cgctttcagc cacgacggcc cggcgagcag gacgatcgcc agcgccacga
     1981 tgaagatgcc gccgggattg aagcgcagtg acgcgacgac gtcgccgtgg agcagcgcca
     2041 cgacggagcg cgtcatgccg cagaacgggc agggaatgcc ggtcgcgctc cgcaacgggc
     2101 acagcagtgg cgggtggatc ggcagcagcg gccacaccgc gccggccgcg atcatccccg
     2161 cggcgaggag acggaactcg cggaacgacc gggtctgcgt ctgcgggatc acgcgcgcgc
     2221 ctgcttcgcg acgacgatgc cttcgagcac gaactcggcg tgcgcgtcgt gcgggacgcc
     2281 gggaaccttc ggccacagtc gccgcacgag ctcggccgcg aagtagctgc ggataggcca
     2341 cggcagcgcg agccggcggt cgaggaagtg gcgcacggtc acgagctcct cgtcgtcgat
     2401 cgcggtgacg tcccaaccct gcacctgcgc gagggggacg gtgatcggcg cgatcggcac
     2461 cggcttgtcc ttgcggccgc cgaaccgttc gcggatgacc agcgtgcccg ccgcgagatc
     2521 cccgagccgc tggctgtgct gcgtggagac gatggagacc gcaccgacga gatagaaccc
     2581 gatgaagtcg atgatgcgca gcaagttccg caccgaactg gtgacgaagt cgacgggttc
     2641 ggcgccgcgg gcgacgacgc ggatgcccat cgctcgcttg cccggtgtcc cgacgccggt
     2701 gacctcgaac gggatgtcgt aggccaacac cacgaggaag atgacgacga tcgcgaccgc
     2761 aatcgcgact cccgtcgaga cgccggtcgc aacgattccg gcaccgaaca ggagtgccag
     2821 aatgatggcc agctggatga gcgagtccag gaggcgggcg acgaatcgcg aaccgagacc
     2881 ggcaatcact acgtcgatcg tgacgccctc cggcgtcgcg atggtgaggc gattgtcgag
     2941 gtccacatgg aaacggtagt tgccgcaccg gccgccggga acgcttcgcc ggtcgagcgg
     3001 ttcgtggccg agcgcgcgcc ggtgtgggac gagctcgagc agctcgtgac ccaggccaag
     3061 aaccgcccgt cgcgagtcgg cgcccacggt gtgcgccgcc tcggggagac gtaccgtgcg
     3121 gccgccgccg acctcgcgat cgcgcgccgc cagtatccgg gcagcccgtt catcgtacgc
     3181 ctggaacgcc tggtgcaacg agcgcgcacc gcggtctacc actcgtcgcg gtcgtcgggg
     3241 acgtttc
//
LOCUS       DASUGA010000781         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_785686, whole genome shotgun sequence.
ACCESSION   DASUGA010000781 DASUGA010000000
VERSION     DASUGA010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785686"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..838)
                     /locus_tag="VFX21_15360"
     CDS             complement(137..838)
                     /locus_tag="VFX21_15360"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEX5097405.1"
                     /translation="MAATNRGTLALVGGGEWETPCRPLDEELLEASGGSDVVVLAAAA
                     AFEHPDRVVERARAWFGDLGAKVEALPVTNRRDAEQDAVVRKAEKARFLYIADGSPLH
                     LRSVLKGSSLFDAIVHAYERGAVLAASGAGATLVCDPMVDPRGGAYTVGLGVVRGLAV
                     FPYHGDAADHLRERSIELLPADATLVGLDAHTALVRREQEEWRVRGPGEATIYRRGDA
                     PIVMRDGASLASASR"
     gene            934..1788
                     /locus_tag="VFX21_15365"
     CDS             934..1788
                     /locus_tag="VFX21_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEX5097406.1"
                     /translation="MTVKRVGIVGSGIMGSGIAEVAAAAGYEVVLRSRAQETADAMVA
                     SLEKSLTKQVEKEKISAADRDATLGRVRAVTDLGELSECDLIIESIGADLDTKKKLFD
                     DLDRICGDQAILATNTSTLPVVEMAMVTGRPDKVCGIHFFNPAPMMALVEVVRAITTS
                     DETIAEATALASACGKNPVQVKDQAGFIVNALLFPYLNNAVRLFDAGVATRDDIDAAM
                     KGGCNFPMGPLQLLDLVGLDTSLAILEALYAEFKDPNYAPAPLLRRMVSAERLGRKTR
                     LGFYDYRK"
     gene            1844..>2187
                     /locus_tag="VFX21_15370"
     CDS             1844..>2187
                     /locus_tag="VFX21_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEX5097407.1"
                     /translation="MSFGAIAIASGLSVLQACALSLLMFTGASQFAFASVISDGGAPF
                     AGAATAALLGVRNALYGLRLTSLLQVRGARKVGAAQLVIDESTAVANAQDDRGDARLG
                     FFATGASVFVLW"
BASE COUNT          353 a          755 c          747 g          332 t
ORIGIN      
        1 cgaccaggcc tacggaccgc aggcgattcc cgaggccggt atcgcgcccg acgagacgtt
       61 gtggttcgtc gtcgacgtca agagcatcga cgacgtcccc ggcgcgagca cgaccgttcc
      121 ggccgacggc aactgatcag cgagacgccg acgcaagcga cgcgccgtcg cgcatcacga
      181 tcggtgcgtc accgcgccgg tagatcgtcg cttcaccggg gcccctcacg cgccactctt
      241 cctgctcgcg gcgaacgagc gcagtgtgcg cgtcgagacc gacgagcgtc gcgtccgcgg
      301 gaagcagctc gatcgagcgc tcacgcaggt gatcggccgc atcgccgtgg tacgggaaga
      361 ccgcgaggcc gcgcacgaca cccaagccga ccgtgtacgc gccgccgcgc gggtcgacca
      421 tcgggtcaca cacgagcgtc gcgcccgcgc ccgaggctgc gagcactgcg ccgcgctcgt
      481 aggcgtgcac gatcgcgtcg aacaacgagc tgcccttgag caccgaccgc aggtgtagcg
      541 gcgagccgtc cgcgatgtag aggaagcgcg ccttctccgc tttgcggacg acagcatctt
      601 gttccgcgtc gcgccggttc gtgacgggaa gtgcctcgac tttggcgccc aggtcgccga
      661 accaggcgcg ggcgcgttcg acgacacggt cgggatgctc gaacgcagcc gcggccgcga
      721 gcaccacgac gtcgctgcca ccggaagcct cgagcagttc ctcgtcgagt ggccggcagg
      781 gcgtctccca ctcgccaccg cccaccaacg cgagcgttcc acgatttgtc gcggccattt
      841 gattccttcg gatgcacgcg gagcgggctg gccgcggcca catacccgtc ggtaacctaa
      901 cgcctttccg tgcaccgcat cgaggagggt gtgatgaccg tcaagcgcgt cgggatcgtc
      961 gggtcgggga tcatgggctc ggggatcgcc gaggtcgccg cggccgccgg ctacgaagtc
     1021 gtgctccgca gtcgcgccca ggagactgcg gatgcgatgg ttgcgagcct cgagaagtcg
     1081 ctgacgaagc aggtcgagaa ggagaagatc tccgcggccg atcgcgacgc cacactcgga
     1141 cgcgtgcgcg cggtcaccga tctcggtgag ctgagcgaat gcgacctgat catcgagtcg
     1201 atcggcgcgg acctcgacac caagaagaag ctgttcgacg acctcgaccg catctgcggc
     1261 gaccaggcga tcctcgcgac gaacacatcg acgctgcccg tcgtcgagat ggcgatggtg
     1321 accggccggc ccgacaaggt gtgcggcatc cacttcttca acccggcgcc gatgatggcg
     1381 ctggtcgaag tcgtgcgggc catcacgaca tccgacgaga cgatcgccga agcgaccgcg
     1441 ctggcgagcg cgtgcggcaa gaaccccgtg caggtcaaag accaggcggg cttcatcgtg
     1501 aacgcactgc tgttcccgta cctgaacaac gcggtgcggt tgttcgacgc gggtgtcgcg
     1561 acccgcgacg acatcgacgc cgcgatgaag gggggctgca acttcccgat gggcccgttg
     1621 cagctcctcg acctggtcgg cctcgacacg agcctcgcga tcctcgaagc gctctacgcc
     1681 gagttcaagg acccgaacta cgcgccggcg ccgctgctgc ggcggatggt cagcgccgaa
     1741 cggctcggcc gcaagacgcg cctcgggttc tacgactatc gcaagtaacc gcagcgtcgt
     1801 ccgcgattcg ctcgcggtcg gtatcgcgac gggcgcgtac ggggtgtcgt tcggcgcgat
     1861 cgcgatcgcg tccgggctgt cggtgctcca agcgtgcgcg ctgtcgctgt tgatgttcac
     1921 cggcgcgtcg cagttcgcgt tcgcgtccgt gatcagcgac ggcggtgcgc ccttcgccgg
     1981 cgcagccacg gccgcgctcc tcggtgtccg caacgcgctg tacggcttgc ggctgacgtc
     2041 gttgctgcaa gtgcgcggcg cacgcaaggt cggcgccgcc cagctcgtga tcgacgagtc
     2101 gaccgcggta gccaacgcgc aggacgaccg cggcgatgcc cgactcggct tcttcgccac
     2161 gggcgcgtcg gtcttcgtgc tctggaa
//
LOCUS       DASUGA010000782         2065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_785708, whole genome shotgun sequence.
ACCESSION   DASUGA010000782 DASUGA010000000
VERSION     DASUGA010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785708"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VFX21_15375"
     CDS             <1..770
                     /locus_tag="VFX21_15375"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HEX5097408.1"
                     /translation="YMHVPAGRVPMALAELHRSLVVGAAVHLQVTCDQLPPEWDDPFT
                     GRHFSYFTTAQFVDVVEGAGFEVVSSGDDGEEWVDVEATRARMLPDTVGSEMRLLLVG
                     LNPSLLSADTGVGFARPGNRFWPAALSSGLATRTHDPYHLLREHGVGMTNLVRRATVG
                     AAELTNEEYREGAARVTRLVEWLRPRAICFVGLTGYRVAVDKKAKLGWQETPFASTPT
                     YVMPNTSGLNAHAKPADFARHLAAAFQPAPFNHPPGG"
     gene            complement(851..1939)
                     /gene="tal"
                     /locus_tag="VFX21_15380"
     CDS             complement(851..1939)
                     /gene="tal"
                     /locus_tag="VFX21_15380"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011627972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX5097409.1"
                     /translation="MASSIAKLLEFGQSPWNDNLTRALARGGLQKLIDEHGIRGVTSN
                     PTIFEKAMGAGTDYDAQLRELLANDPDTTKAYWTLVEQDIADAADILRPVYEEADGTD
                     GFVSIEVDPDLAHDSEGTLTQVKDFFGRLARPNVMIKIPATREGLPAITDATAAGVNV
                     NITLIFSLERYEEVIEAFFEGLEQRVERGEDISHISSVASFFVSRVDTETDRRLPDPH
                     PLRGKAAVANAKLAYQVFVDRFSGPRWNDLAAAGAHKQRPLWASTSTKNPAYSPTLYV
                     DTLVGRDTVNTLAQASIDVLADYQGLRPDTVLEGVDEAQAVIDGLADAEVDFADVTAT
                     LEREGVAAFSASYHDALDTLAKKSRELK"
BASE COUNT          288 a          701 c          716 g          360 t
ORIGIN      
        1 cgtacatgca cgtccctgcc ggacgcgtgc cgatggcact ggcggagctg caccgctcgc
       61 tcgtcgtcgg cgcggccgtg cacctgcagg tgacgtgcga ccaactgccg ccggagtggg
      121 acgatccctt cacgggccgg cacttctcgt acttcacgac cgcccagttc gtcgacgtcg
      181 tggagggcgc aggcttcgag gtggtttcga gtggcgacga cggtgaggag tgggtcgacg
      241 tcgaggcgac gcgggcgcgc atgctgcccg acaccgtggg ttccgagatg cggttgctcc
      301 tggtcggatt gaacccgagc ctgctgtcgg ccgacaccgg cgtcgggttc gcgcgtccgg
      361 gtaaccggtt ctggcccgcg gcgttgtcga gcggcctcgc gacgcgtacg cacgatccgt
      421 accacttgtt gcgcgagcac ggcgtcggca tgacgaacct cgtgcgccgc gcgactgtcg
      481 gcgcggcgga gctgacgaac gaggagtacc gcgaaggtgc ggcccgcgtc acccgtctcg
      541 tcgagtggct ccggccgcgc gcgatctgct tcgtcggcct caccggctac cgcgtcgccg
      601 tcgacaagaa ggccaagctc ggctggcagg aaacgccgtt cgcgagcacg ccgacctatg
      661 tgatgcccaa caccagcggc ctcaacgccc acgccaaacc cgccgacttc gctcgccacc
      721 tcgcggctgc gttccaacct gcgcctttca accacccgcc cggtggttga acggcgccgg
      781 ttcgatgggg ccgcgtactt gcgccgctca accacccgtc cggtggttca gcggcgcagg
      841 tacgcgcgga ctacttgagc tcgcgggact tcttcgcgag ggtgtcgagg gcgtcgtggt
      901 acgacgcgct gaaggcggcg acgccttcgc gttcgagggt ggcggtgacg tcggcgaagt
      961 cgacctcggc gtcggcgagg ccgtcgatga ccgcctgggc ctcgtcgacc ccttcgagca
     1021 cggtgtcggg gcgcaacccc tggtagtcgg cgaggacgtc gatcgacgcc tgcgcgagcg
     1081 tgttcacggt gtcgcggccg acgagcgtgt cgacgtacaa cgtcggcgag tacgcggggt
     1141 tcttggtcga cgtcgaggcc cacagcggtc gctgcttgtg cgcgccggcg gccgcgaggt
     1201 cgttccagcg cgggcccgag aaccggtcga cgaacacttg gtacgcgagc ttcgcgttcg
     1261 cgaccgcggc cttgccgcgc agcgggtgcg ggtcggggag ccggcggtcg gtctcggtgt
     1321 cgacccggct gacgaagaac gacgcgaccg acgagatgtg cgagatgtct tcgccgcgct
     1381 cgacgcgctg ctcgagccct tcgaagaacg cctcgatcac ttcctcgtag cgctcgaggc
     1441 tgaagatcaa cgtgatgttg acgttgacgc cggccgcggt ggcatcggtg atcgcgggca
     1501 gaccctcgcg cgtcgcggga atcttgatca tgacgttcgg gcgcgcgaga cggccgaaga
     1561 agtccttgac ctgcgtgagc gtgccttcgg agtcgtgcgc gaggtcgggg tcgacctcga
     1621 tcgacacgaa cccgtcagtg ccatcggcct cttcgtacac gggccggagg atgtcggccg
     1681 cgtcggcgat gtcttgctcg acgagcgtcc agtacgcctt cgtcgtgtcg gggtcgttcg
     1741 ccaacagctc gcgcagctgc gcgtcgtagt cggtgcccgc gcccatcgcc ttctcgaaga
     1801 tcgtcgggtt cgacgtgacg ccgcggatgc cgtgctcgtc gatcagcttc tgcagcccgc
     1861 cgcgcgcgag ggcgcgcgtg agattgtcgt tccacgggct ctgcccgaac tccaacagct
     1921 tcgcgatcga actcgccatc tcaatctcca tcctcacggg tcgactccac gctccacctt
     1981 ctgaacgtgg gcttcgccgg atgtcatccg gctccgctgt cgtctcccaa cagcgccata
     2041 gcccgcgccg cgacgttttc ggggc
//
LOCUS       DASUGA010000783         4812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_368896, whole genome shotgun sequence.
ACCESSION   DASUGA010000783 DASUGA010000000
VERSION     DASUGA010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368896"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VFX21_15385"
     CDS             <1..462
                     /locus_tag="VFX21_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097410.1"
                     /translation="LPDRFAAVAPVAGVEFYDDCAAGRVAVMAFHGTADPIVTYDGGG
                     LNATAIAASNSWNGDVPPGLPVHHGVDAAMKTWAAHNGCDAEPETEDVAPSVRRRTWS
                     GCDAPTVLYIIDGGGHAWPGKPVPEFEAQFGAGTTAVDASALIFAFFLGPH"
     gene            840..1256
                     /locus_tag="VFX21_15390"
     CDS             840..1256
                     /locus_tag="VFX21_15390"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097411.1"
                     /translation="MLWSDGGHKDVWNARYSPNGNRAAAVGADGVLSVYEAKTGRVLY
                     RVYAPKKTPLHALDWSPDGTLLAAGGGDKNVYVYNAANGALYDTLVGHKTSVSAVAWS
                     VDSRTLATAAGGQRLSVALLDTRVGPDDAIHLWTRK"
     gene            complement(1257..1721)
                     /locus_tag="VFX21_15395"
     CDS             complement(1257..1721)
                     /locus_tag="VFX21_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097412.1"
                     /translation="MPDIDDSLFDEVADVLAGLVPDDLGPVHQQPRRWGIKVWFGGDT
                     VTKEHYEAQVVSRDAVPGATVLALEIGFHAEHPKVAENDAVLAKLVAGETKWRKTLGA
                     EPVAGPFLGRADVWRRVSETWPDPDLSEPGLAFEVGARLTDYIAALEPLRRK"
     gene            complement(1723..2664)
                     /locus_tag="VFX21_15400"
     CDS             complement(1723..2664)
                     /locus_tag="VFX21_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020400928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX5097413.1"
                     /translation="MTADARLPQLDADIFLSDGGIETTLIFLDGFDLPEFAAFPLLDD
                     AAGRTALMRYFESYAEIARRDGVGIVLETPTWRASPAWGAKLGYSGADLERINRDAVA
                     LVEEVRAKYATDASPIVISGCIGPSGDGYRPESLMTREEAREYHSLQARAFADAGADL
                     ITAITMTHTAEAIGIADAARAVELPVVLSFTVETDGVLPSGERLGEAIEHTDAATGAY
                     PSYYMVNCAHPTHFVDVLDDAQSWSRRVRGIRANASRMSHDELDNAEELDRGDPAELA
                     ALYRDLRDTHPQLTVLGGCCGTDGEHIGAISAACALR"
     gene            complement(2801..3613)
                     /locus_tag="VFX21_15405"
     CDS             complement(2801..3613)
                     /locus_tag="VFX21_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007226204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX5097414.1"
                     /translation="MTIDVQPLTPTIGAEVYGIDLGHLCDTGTLAELQKAWLDYKVLF
                     FREQHITTDQHIAFCRQFGDLEVHPFVPPKPGYPEVMVLIANDKRKGSENAWHSDVTW
                     RQEPSLGSMLRAVEIPPIGGDTLFADMEAAYGNLPESIKEQLDGRTAIHDFARVFGVV
                     DEAKRAELREKYPPAEHPVIRTHPVSGRKSIYVNDGFTSHIVGMDPDESTKLLRLLYR
                     QAAVPEYQCRLRWAPDTFALWDNRSAQHYAVSDYFPQTRVMERVTIVGDRPR"
     gene            3759..>4812
                     /locus_tag="VFX21_15410"
     CDS             3759..>4812
                     /locus_tag="VFX21_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX5097415.1"
                     /translation="MQGTVVSISVAPGDAVRAGEPVLVLESMKMEHVVASAWDGVVEE
                     IPVTIGQTVMEGDPLARVAPAAAPTPDRFAAESAVDLDHVRADLAEVLERHDVGLDHR
                     RAEAVTKRHASGKRTARENIADLLDDGSFVEYAPLVVAAQRRRRSMEELIARTPADGL
                     VGGIGTIAGQPVIAVSYDYMVLAGTQGLWNHAKKDRLFELAERLRAPVVLFAEGGGGR
                     PGDTDGIGVSGLDCLAFNYFAKLSGLVPLIGITTGYCFAGNAALLGCCDVVIATDGSN
                     IGMGGPAMIEGGGLGVYEPTAIGPIEVQEANGVVDVRVRDEAEAVRVAQQYLTYFSGP
                     AASWTSVDQRTLRHLVP"
BASE COUNT          707 a         1664 c         1693 g          748 t
ORIGIN      
        1 ctgcccgacc ggttcgccgc ggtcgcgccg gtcgcgggcg tcgagttcta cgacgactgc
       61 gcggcgggac gggtcgcggt gatggcgttt cacggcaccg ctgatccgat cgtcacctac
      121 gacggcggcg ggctgaacgc gaccgcgatc gcggcgagca actcctggaa cggcgacgtg
      181 cccccggggc ttccggtaca ccacggcgtc gacgccgcga tgaagacttg ggcggcgcac
      241 aacggctgcg acgccgaacc cgagaccgag gatgtcgcgc cctcggtgcg ccgccgcacg
      301 tggtcgggct gcgacgcgcc gaccgtcctc tacatcatcg acggcggcgg tcatgcgtgg
      361 cccggcaagc cggtgcccga gttcgaggca cagttcgggg cggggaccac cgcggtcgac
      421 gcctcggcgc tgatcttcgc tttcttcctc ggcccgcact gacccagaac ttcccgggcc
      481 ggccgcacta tcgccccact tcccgcatgt cggtacgctc cgttcggatt tgggcgtccc
      541 accgaaggga cctcagggat gacgcaggtg gggggaagcg tcggctcgag ccgcaggacg
      601 cggcgggccg ggcgctggtc ggtagccgtc gcggcggtgc cggcactgct cgcgggcgcg
      661 ttcgcggccg caacaccggc accggcaccg ccgccggatc gttcgtcgtc gtcgcacttc
      721 acgcccgcgg gccagatcac gacgcccggt cggccgctgt cgatcgcctg gtcgcgagcg
      781 tcaactggag gggccagccg gcgtggttca acaagaacgc ggcacgcctc gcgggcaacg
      841 tgctgtggag cgacggcggg cacaaggacg tgtggaacgc gcgctactcg cctaacggca
      901 accgcgccgc cgcggtcggc gccgacggtg tgctcagcgt gtacgaggcg aagaccggtc
      961 gcgtgctcta ccgcgtgtac gcaccgaaga agacgccgct ccatgcgctc gactggagcc
     1021 ctgacggcac gttgctcgcg gccggcggag gagacaagaa cgtctatgtc tacaacgcgg
     1081 cgaacggcgc gctgtacgac accctcgtcg ggcacaagac gtcggtcagc gcagtggcgt
     1141 ggtcggtcga cagcaggacg ctggccactg cggcgggcgg tcagcgcctc tcggtggcac
     1201 tcctcgatac gcgcgtgggg cccgacgacg cgatccacct ctggacgcgg aagtagtcac
     1261 ttccggcgca gcggttcgag cgcggcgatg tagtcggtga ggcgcgcgcc gacctcgaac
     1321 gcgagacccg gttcgctcag gtcgggatcc ggccacgtct ccgacacgcg ccgccacacg
     1381 tcggcgcggc cgaggaacgg gcccgcgacc ggttcggcgc cgagcgtctt tcgccacttc
     1441 gtctcgcccg cgacgagctt ggcgagcacc gcgtcgttct ccgcgacctt cgggtgctcg
     1501 gcgtggaacc cgatctcgag cgccagcacg gtcgccccgg gtaccgcgtc gcgcgacacg
     1561 acctgcgcct cgtagtgctc cttggtcacg gtgtcgccgc cgaaccagac cttgatgccc
     1621 caccggcgcg gctgctggtg caccgggccg aggtcgtcgg gcacgagtcc cgcgagcacg
     1681 tcggcgacct cgtcgaacag cgagtcgtcg atgtcgggca tctcagcgga gcgcacacgc
     1741 ggcgctgatc gcgccgatgt gttcgccgtc ggtgccgcaa cacccgccga gcaccgtgag
     1801 ttgcgggtgc gtgtcgcgta ggtcgcgata cagcgccgcg agctcggcgg gatcgccgcg
     1861 gtcgagctct tctgcgttgt cgagctcgtc gtgactcatg cgggacgcgt tcgcccggat
     1921 gccgcgcacg cgccgtgacc acgactgcgc gtcgtcgagg acgtcgacga agtgtgtcgg
     1981 gtgcgcacag ttgaccatgt agtacgacgg atacgcaccg gtcgccgcgt ccgtgtgctc
     2041 gatcgcctcc cccagccgct cgcccgacgg caggacaccg tcggtctcga ccgtgaacga
     2101 cagcaccacg ggcagctcga ccgcgcgagc ggcgtccgcg atgccgatcg cttctgcggt
     2161 gtgcgtcatc gtgatcgcgg tgatcaggtc ggcacccgcg tcggcgaacg cgcgcgcctg
     2221 caacgagtgg tactcgcgcg cctcctcgcg cgtcatgagc gactccggcc ggtatccgtc
     2281 gccgctcgga ccgatgcatc cgctgatgac gatcggtgag gcgtccgtcg cgtacttcgc
     2341 acgcacttct tcgacgagcg cgaccgcatc gcggttgatc cgctcgaggt cggcgccgga
     2401 gtacccgagc ttcgcgcccc aggccgggct cgcgcgccac gtcggcgtct cgagcacgat
     2461 gccgaccccg tcgcgccgcg cgatctcggc gtagctctcg aagtagcgca tgagcgcggt
     2521 gcgaccggcc gcatcgtcga gcagcgggaa ggcggcgaac tcgggaaggt cgaaaccgtc
     2581 gaggaagatc agcgtggtct cgataccgcc gtcactcaag aagatgtcgg catcgagttg
     2641 cgggagacga gcgtccgcgg tcacggcgag agactgcacc atcccgcgcc cgggaagcca
     2701 tacccgggtt cgcgacggcg accaggtggt ctggcagtac ggccccgcct tcaccgcgag
     2761 ggccgaacga gtctgacgat cagaggtcag ccgcgccctg ctagcgaggg cggtcgccga
     2821 cgatcgtgac gcgctccatc acgcgcgtct gcgggaagta gtcgctgacc gcgtagtgct
     2881 gcgcgctgcg gttgtcccag agcgcgaacg tgtctggcgc ccagcgcaac cggcactggt
     2941 actcggggac cgccgcctgc cggtagagga ggcgcagcaa cttcgtgctc tcgtcgggat
     3001 ccatgccgac gatgtgcgac gtgaacccgt cgttgacgta gatcgacttg cggcccgaca
     3061 ccggatgcgt gcggatcacc ggatgttcag cgggcgggta cttctcgcgc agctcggccc
     3121 gcttcgcctc gtcgaccact ccgaagacgc gcgcgaagtc gtggatcgcg gtgcggccgt
     3181 cgagctgttc cttgatcgac tcggggagat tgccgtacgc ggcttccatg tcggcgaaca
     3241 acgtgtcgcc gccgatcggc gggatctcca ccgcgcggag catcgacccg agcgacggct
     3301 cctggcgcca cgtgacgtcg ctgtgccagg cgttctcgct gcccttccgc ttgtcgttcg
     3361 cgatgaggac catcacttcc gggtagccgg gcttgggcgg cacgaacgga tgcacttcga
     3421 ggtcaccgaa ctggcggcag aacgcgatgt gctggtcggt ggtgatgtgc tgctcgcgga
     3481 agaacagcac cttgtagtcg agccaggcct tctgcagctc cgcaagggtg ccggtgtcgc
     3541 agaggtggcc gagatcgatg ccgtacacct cggccccgat cgtcggtgtg agcggctgca
     3601 catcgatcgt catctctttg tcgctccggt cgtgctcgcg tgaggcggcc cggattgtcg
     3661 gcccgcctcc gctccgcggg tgcctccgca ggctacggca ccgcgccgtg cgactgcagt
     3721 gcgctatcac tgccctcgtg gaaaccgtct tggctccgat gcagggaacc gtcgtatcca
     3781 tctcggttgc accgggagac gcggtgcgag cgggagagcc ggtgctggta ctcgagtcca
     3841 tgaagatgga gcacgtcgtc gcgtcggcat gggacggagt cgtcgaggag atcccggtca
     3901 cgatcggcca gaccgtcatg gaaggcgacc cgctcgcccg ggtcgcgccg gcagccgcgc
     3961 caacaccgga ccggttcgcc gccgaatccg ccgtcgatct cgatcatgtt cgcgccgatc
     4021 tcgccgaggt gctcgagcgc cacgacgtcg ggctcgacca ccgccgcgcc gaggccgtca
     4081 ccaaacggca cgcgtcgggg aagcgcaccg cgcgcgagaa catcgccgac cttctcgacg
     4141 acggatcgtt cgtggagtac gcgccgctcg tcgtcgccgc gcaacgccgg cggcgctcga
     4201 tggaagagct catcgcgcgc acgccggccg acgggctcgt cggtggcatc ggcaccatcg
     4261 ccggacaacc ggtgatcgcg gtgtcgtacg actacatggt gctcgccggt acgcagggcc
     4321 tgtggaacca cgcgaagaag gaccggctct tcgagctcgc ggagcgcctg cgcgcacccg
     4381 tcgtgttgtt cgccgaaggc ggcggcggac ggccgggaga caccgacggc atcggtgtgt
     4441 ccggcctcga ctgcctcgcg ttcaattact tcgcgaagct gagcgggctc gtcccgttga
     4501 tcggcatcac gaccggctat tgcttcgcgg gcaacgcggc gctgctcggg tgttgcgacg
     4561 tcgtcatcgc gaccgacggc tcgaacatcg ggatgggcgg tccggccatg atcgagggcg
     4621 gcgggctcgg cgtctacgaa ccgaccgcga tcgggccgat cgaggtgcag gaggcgaacg
     4681 gtgtcgtcga cgtgcgcgtc cgcgacgaag ccgaggccgt acgcgtcgcg cagcagtacc
     4741 tcacgtattt ctccggcccg gccgcgtcgt ggacgtccgt cgaccagcgc acgctccgcc
     4801 acctcgtccc gg
//
LOCUS       DASUGA010000784         2153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2036522, whole genome shotgun sequence.
ACCESSION   DASUGA010000784 DASUGA010000000
VERSION     DASUGA010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2036522"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VFX21_15415"
     CDS             <1..453
                     /locus_tag="VFX21_15415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5097416.1"
                     /translation="ELRVTLAMLRDEPAAPRAPTPGLAELRSLVAGAERAGLPVEVRV
                     EGAVRDLPPAIDTAAFRIVQESLTNVMRHAGPARACVAVVYAPDGVEIEVVDDGVGLG
                     GANGSHAGHGIRGMHERASMVGGRVEAGPRPEGGFRVRAHFPSEVMSS"
     gene            450..1115
                     /locus_tag="VFX21_15420"
     CDS             450..1115
                     /locus_tag="VFX21_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5097417.1"
                     /translation="MIHVVVADDQDLVRAGLRVMLEAEDDIAVVAEAINGVDVIEQVR
                     AASPDVVLMDIRMPELDGLEATRIISEDPALEDVKVLVLTTFELDEYVFEALRAGASG
                     FLLKDVEASELLRAVRVVHDGESLLAPSVTRRLIDEFVARPERVRASSALDELTDRER
                     EVVALVAAGLTNEEIAEHLVISPATARTHVSRAMVKVGARDRAQLVVLAYESGLVAPG
                     RSS"
     gene            1212..1973
                     /locus_tag="VFX21_15425"
     CDS             1212..1973
                     /locus_tag="VFX21_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020140476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5097418.1"
                     /translation="MPSVIASTELQTETLAARTVGLVKDYGTAETAVRALDGVTIGVP
                     RGRFTAIMGPSGSGKSTLLHCLAGLDAPTAGRVFIGDVDLTELSDRQLTLLRRSTLGF
                     VFQSFNLVPTLTALENITLPMSLAAEKPDEDWLTEVIATVHLRDRLSHRPSELSGGQQ
                     QRVAVARALVSRPAIVFADEPTGNLDSRSGTQVLELLRDAVDRIDQTVVMVTHDPAAA
                     AYADDVVFLADGRVVDQMAEPTAAAVLDRMKSFGE"
BASE COUNT          314 a          737 c          744 g          358 t
ORIGIN      
        1 gaactgcgcg tcacgctggc catgctgcgc gacgagccgg ctgcacctcg agctcccacg
       61 ccggggctcg ccgagctccg gtcgctcgtc gcgggcgcgg aacgcgccgg cctccccgtg
      121 gaggtgcggg tcgagggagc agtgcgcgac ctgccgcccg ccatcgacac cgccgccttt
      181 cgcatcgtcc aggagtcgct caccaacgtg atgcggcacg ccggtccggc ccgcgcgtgc
      241 gttgccgtgg tgtacgcgcc ggatggcgtc gagatcgaag tggtcgacga cggcgtggga
      301 ttgggaggcg cgaacggttc gcacgcgggc cacggcattc gcggcatgca cgaacgcgcg
      361 tcgatggtcg gcggtcgggt ggaagcgggt ccccgtcccg agggtggctt ccgtgtccga
      421 gcccactttc ccagcgaggt gatgtcgtca tgatccatgt cgtcgtggcg gacgaccaag
      481 acctggttcg tgccggcctt cgcgtcatgc tcgaagcgga ggacgacatc gccgtcgtcg
      541 ccgaagcgat caacggcgtc gacgtcatcg agcaggtccg cgccgcctcg ccggacgtgg
      601 tgctcatgga catccgcatg cccgagctcg acggcctcga ggccacgagg atcatctccg
      661 aggatcccgc gctggaggac gtcaaggtgc tcgtcctcac caccttcgag ctcgacgagt
      721 acgtcttcga ggccctgcgc gccggcgcga gcggcttcct gctgaaagac gtcgaggcca
      781 gcgagctgct tcgagcggtg cgggtggtgc acgatggcga gtcgttgctc gcgccgagcg
      841 tcacccgccg gctgatcgac gagttcgtgg cccgacccga gcgggtgcgc gcctcgtcgg
      901 cgctcgacga gctcaccgac cgcgagcgcg aggtcgtcgc gctggtggcg gcgggcctca
      961 ccaacgagga gatcgccgag cacctggtga tcagccccgc tacggcacgt acgcacgtga
     1021 gccgggccat ggtgaaggtc ggggcgcgcg atcgagccca gctggtggtc ctcgcgtacg
     1081 aatcgggcct cgtcgccccg ggccgctcgt cgtgagtacg tcgagcgcgc gtcacctacg
     1141 tcgctcgcgg taggcacaag tgtcttcgct cgcacgacga caggtacgcc gcgcttcccg
     1201 acgatggagg catgccatca gtcattgcct ccaccgaact gcaaaccgaa accctcgcgg
     1261 cccgcactgt gggactggtg aaggactacg gaaccgccga gacggccgtg cgtgcgctcg
     1321 acggtgtgac gatcggagtt cctcgcggtc gcttcaccgc gatcatgggt ccgtccggat
     1381 cgggcaagtc gacgctcctg cactgcctgg ccgggctcga cgcgccgacg gccggtcggg
     1441 tcttcatcgg tgacgtcgac ctcaccgagc tctccgatcg acagctgacc ttgctgcgtc
     1501 gcagcacgct cggcttcgtc ttccagtcgt tcaacctcgt tcccacgctg acggcgttgg
     1561 agaacatcac gcttccgatg tcgctggccg ccgagaagcc cgacgaggac tggctgaccg
     1621 aggtgatcgc caccgtgcac ttgcgcgatc gactctcgca ccgcccgtcc gagctgtcgg
     1681 gtggtcagca acagcgcgtc gcagtggcgc gtgccctcgt gagccgtccc gcgatcgtct
     1741 tcgccgacga gccgaccggg aacctggact cacgatccgg cacccaggtg ctggagctgt
     1801 tgcgcgacgc cgtcgaccgg atcgaccaga cggtcgtgat ggtcacacac gacccggcgg
     1861 ccgcggcgta cgccgacgac gtcgtgttcc tggccgacgg tcgcgtcgtc gaccagatgg
     1921 ccgaacccac tgccgcggcg gtgctcgacc gcatgaagtc gtttggagag tgagggaccg
     1981 tcatgtttcg tgtgagcctt cgtgacatct ggagtcggaa gcggcgcctg ctcagcacgt
     2041 cgctggccgt cgtgctcggc gtcgccctgc tagccggcac gctcgtgttg ggtcagacgt
     2101 tggagtcgaa cttcgatcgg ttgttcgccg acgcgaccgc aggcaccgac gtc
//
LOCUS       DASUGA010000785         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_369196, whole genome shotgun sequence.
ACCESSION   DASUGA010000785 DASUGA010000000
VERSION     DASUGA010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369196"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VFX21_15430"
     CDS             <1..798
                     /locus_tag="VFX21_15430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX5097419.1"
                     /translation="LPDQQPFTEEDERVLTELARQVALALHNSALDTALQASLDELRV
                     TNEELRQSRARIVATADQSRRQIERNLHDGAQQHLVALAVKLGLARQMVGGDTDTLST
                     LLEELRADAQTTLTELRELAHGIYPPLLMDRGLCEALRAAANRSVLATDVDADVGRFP
                     PEVEAAVYFCVLEALQNAGKHAGEGARLTVNVTATDHELNFSVHDDGAGFDTTTGAIR
                     GHGFVNMEDRLGAIGGVLKVESAPGAGTTISGEIPLTVSAVRDVAPD"
     gene            complement(769..1173)
                     /locus_tag="VFX21_15435"
     CDS             complement(769..1173)
                     /locus_tag="VFX21_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006539977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5097420.1"
                     /translation="MDAMNDMEPATKTVSVLIVDDQKPFRDVANTVVRMTAGFEVTGE
                     AESAEDAIDAVDARPPDLVLMDINLPGINGIEACARITAAHPDVHVILLSTYSEQDLP
                     QDARACGALRYVHKEEFSPALLQSIWGDVADR"
     gene            complement(1249..1911)
                     /locus_tag="VFX21_15440"
     CDS             complement(1249..1911)
                     /locus_tag="VFX21_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5097421.1"
                     /translation="MVVAEDNLLVREGVVTLLSNQPELEVAGSVGSYDELIETVEREQ
                     PDVVVTDIRMPPTGTDEGIRAALTLRDTHPRTAVVVLSQYVDPAYALALLERGSESRA
                     YLLKERVSDPAQIVHAIEETARGGSVIDPMVVESLVAQRAKAPSALERLTPREREVLE
                     QMAQGRNNAGIAQALFLSERAVEKHINSLFSKLGLSEEPDVHRRVKAVLLFLAEAPAQ
                     GG"
BASE COUNT          326 a          666 c          690 g          338 t
ORIGIN      
        1 ctgcccgacc agcagccctt caccgaggaa gacgaacggg tgctgacgga gttggcccgt
       61 caggtcgcgc tcgcgctgca caactccgcc ctcgacaccg cgctgcaagc gtcgctcgac
      121 gagctgcggg tgacgaacga agagctgcgg caatcacggg cccgcatcgt cgcgacggcc
      181 gaccagtcgc gccgccagat cgaacgcaac ctgcacgacg gcgcgcaaca gcacctggtc
      241 gcgctggcgg tgaagctcgg gctcgcgcgc cagatggtcg gcggcgacac cgacacgctt
      301 tcgaccttgc tcgaagaact gcgcgccgac gcgcagacca cgctcaccga gctgcgcgaa
      361 ctcgcgcacg gcatctatcc gccgttgctg atggaccgcg gcttgtgcga agcgttgcgt
      421 gcggcggcga accgttcggt tttggcgacc gatgtcgacg ccgatgtcgg ccggttcccg
      481 cccgaggtcg aggcggccgt ctacttctgc gtgctggaag cgctgcagaa cgcaggcaag
      541 cacgccggtg aaggggcgcg gctcacggtc aacgtgacgg cgaccgacca cgaactcaac
      601 ttctcggtgc acgacgacgg cgcaggcttc gacacgacga ccggcgcgat ccgcggccac
      661 ggtttcgtca acatggaaga ccggctcgga gcgatcggcg gcgtgttgaa ggtggagagc
      721 gcacccggcg cgggcacgac gatcagcggt gagatcccgc tgacggtgtc agcggtccgc
      781 gacgtcgccc cagattgatt gcaacagcgc gggactgaac tcttccttgt gcacgtagcg
      841 caacgcgccg cacgcacggg cgtcttgcgg caggtcctgc tcggagtacg tcgacaagag
      901 gatgacgtgc acgtcgggat gcgcggccgt gatccgggcg caggcctcga tgccgttgat
      961 gccgggcagg ttgatgtcca tcaacacgag atctggaggc cgggcgtcga cggcgtcgat
     1021 ggcgtcttcc gcgctttcgg cctcgcccgt cacctcgaat ccggcggtca tcctcacaac
     1081 cgtgttggcg acgtcgcgga acggcttttg gtcgtcgacg atcaagacgc tgaccgtctt
     1141 cgtcgccggt tccatgtcgt tcatcgcatc catggtcgca ccaatcactg cctgctccca
     1201 gggtgccagc ctcccaatca ccgggggatc accctccggc gggtgaggtc atccgccttg
     1261 ggcgggagcc tcggcgagga acagcagcac tgccttcact cggcgatgaa cgtcgggttc
     1321 ttcggaaagg ccgagtttcg agaacaggga attgatgtgc ttctcgacgg cccgctccga
     1381 caggaacagg gcctgggcga tgccggcgtt gttgcggccc tgcgccatct gttcgaggac
     1441 ctcccgttcc cggggggtga ggcgctccag ggcgctcggt gccttggccc gctgggcaac
     1501 cagcgattcc acgaccatcg ggtcgatgac gctgccgccc cgggcggtct cctcgatcgc
     1561 gtgcacgatc tgggccggat ccgagacccg ctctttgagg aggtaggccc ggctttcgga
     1621 accccgttcg aggagcgcga gggcgtaggc cgggtcgacg tactggctga gaacgacgac
     1681 cgcggtgcgc gggtgcgtgt cacgaagcgt gagtgcggcg cgaatgcctt cgtcggtgcc
     1741 ggtcggcggc atccggatgt cggtcacgac cacgtcgggc tgctcgcgct cgacggtttc
     1801 gatgagctcg tcgtagctgc cgaccgagcc cgccacctcg agctcgggct ggttcgagag
     1861 caaagtgacg acgccttccc ggaccagcag attgtcctcg gcgaccacca cccgtaacgc
     1921 cacggagtac gacgttaccg gccggtgggt gctggagcgc gtccgctagg atctcccggt
     1981 tcccgaagcc cttttggagt tggtcatgta cgcggtcatc
//
LOCUS       DASUGA010000786         2595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_90701, whole genome shotgun sequence.
ACCESSION   DASUGA010000786 DASUGA010000000
VERSION     DASUGA010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90701"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1671)
                     /locus_tag="VFX21_15445"
     CDS             complement(<1..1671)
                     /locus_tag="VFX21_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097422.1"
                     /translation="MSTGTAGRRRGRVRRGPFAVIPIFVVLALALGACTSSDDDDDNA
                     SGDDTTTTASGSNSNLPLTYAAAKADGKEGDIEWGDQCDTEIGRLKVPVVNAPPCVEP
                     YTGTDNGGATAQGVTADEILVAVYKGQPDPLQQAIIEGAGADTDPNTINQTTIDYLKM
                     FEDIVETYGRTLKIETIEATGGPADSTAALADAQKAIALKPFAVVGGPAQTPAWWQEI
                     TNAKIVCVGGCSLAQTQDIVDKAAPYLWPTGPAPEQADAHLEELVGKQLAGKPVEYAT
                     DALNGKDRVYGWIQAETQTGQYQQRIEAAEKNLKDDYDVDISADYTYLYDPANAQQIS
                     TTAVARMKDAGVTTVILSVDPLIPADITKEATKQNYFPEWVLGPNVLMDTTIFGRRTD
                     QDQWSHMIGISLPTARGQREQSDSYLSYQWYYGKEVPVNSQAVLYPAPWQLLVGISLA
                     GPNLTPETYRDGLFNYPSQEGLITSTYVSWGNDLWPTTDYNSSDDSTAIFWDRAATGK
                     DEAGNEGTGMVAYVDGGKRYLPGQWPTEKIPFYDDPDAVTIYDQRPDAA"
     gene            complement(1685..>2595)
                     /locus_tag="VFX21_15450"
     CDS             complement(1685..>2595)
                     /locus_tag="VFX21_15450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5097423.1"
                     /translation="PDVLRSVFLEGAATYDTDKEAQAPTIPTIEVRDADVDLASAPSG
                     NGSSAPRLEVFDISKRFGGINALTDVSFDVRAGEILGFLGPNGAGKTTLFDVLSGFHV
                     ADRGNVLLEGSSIARLGPDARARRGLGRSFQDGRLFPALTVTETITVACDRAYEQRLR
                     GKAKRARVDELIELLGLQAFRHKYIRELSTGSRRVVDLACVLAHNPRVLLLDEPSSGI
                     AQREAEALGPLLLRIRAMTGASLLVIEHDVPLLTSITDRMLALDLGEVVAIGTAEEVV
                     HDPRVVASYLGESEAVVARSGPRDGQ"
BASE COUNT          367 a          854 c          889 g          485 t
ORIGIN      
        1 cgcggcgtcg gggcgctggt cgtagatcgt gaccgcgtcg gggtcgtcgt agaaggggat
       61 cttctccgtc ggccactgac cgggcaggta gcgcttgccg ccgtcgacgt aggcgaccat
      121 gcccgtgccc tcgttgccgg cttcgtcctt cccggtcgcc gcgcggtccc agaagatcgc
      181 ggtggagtcg tcggaggagt tgtagtcggt ggtcggccac aggtcgttgc cccacgacac
      241 gtatgtcgac gtgatgaggc cttcctgtga cggatagttg aacaagccgt cgcggtacgt
      301 ctccggcgtg aggttcgggc ccgcgaggct gatgccgacc agcagctgcc acggcgccgg
      361 atagagcacc gcctgcgagt tcaccggcac ttccttgccg tagtaccact ggtacgacaa
      421 gtacgagtcg ctctgctccc gctgaccgcg ggcggtcggc aacgagatac cgatcatgtg
      481 cgaccactgg tcctggtcgg tgcggcgacc gaagatcgtc gtgtccatca acacattcgg
      541 gccgagcacc cactcgggga agtagttctg cttcgtcgct tccttggtga tgtcggcggg
      601 aatcaacggg tcgacgctga ggatgacggt cgtcacgccc gcgtccttca tgcgggcgac
      661 cgccgtcgtc gagatctgct gggcgttggc cgggtcgtag agatacgtgt agtcggccga
      721 gatgtcgacg tcgtagtcgt ccttcaggtt cttctcggcc gcttcgatgc gctgctggta
      781 ctgaccggtc tgcgtctcgg cctggatcca gccgtacacg cggtccttgc cgttgagcgc
      841 gtcggtcgcg tactcgaccg gcttgcccgc gagctgcttg ccgacgagct cttcgaggtg
      901 tgcgtcggcc tgttcgggcg cgggaccggt cggccacagg tagggcgccg ccttgtcgac
      961 gatgtcctga gtctgggcga gcgagcaccc gccgacgcac acgatcttgg cattggtgat
     1021 ctcctgccac cacgccggtg tctgcgcggg accgccgacc accgcgaacg gcttcagcgc
     1081 gatcgccttc tgcgcgtccg cgagcgcggc cgtcgagtcc gcggggccgc cggtggcttc
     1141 gatcgtctcg atcttgagcg tacggccgta cgtctcgacg atgtcctcga acatcttgag
     1201 gtagtcgatc gtcgtctggt tgatcgtgtt cgggtcggtg tccgcgcccg cgccttcgat
     1261 gatcgcctgt tgcaacgggt cgggctgacc cttgtagacc gcgaccaaga tctcgtcggc
     1321 ggtgacaccc tgtgccgtcg caccgccgtt gtcggtgccc gtgtagggct cgacgcacgg
     1381 cggcgcgttc acgaccggca ccttcaggcg gccgatctcg gtgtcgcact ggtcgcccca
     1441 ctcgatgtcg ccctctttgc cgtcggcctt ggccgccgcg tacgtgagcg gcaggttgct
     1501 gttcgagccg ctcgccgtcg ttgtcgtgtc gtctcccgac gcgttgtcgt catcgtcgtc
     1561 gctactcgtg cacgcgccga gcgcgagcgc gagcacgacg aagatcggta tgacggcgaa
     1621 cggtccgcgc cggacgcgcc cgcgccgacg accagcggtg cctgtactca tggttccccc
     1681 aggatcactg cccgtcgcgg ggaccgcttc gggcgacgac tgcttcggat tcaccgaggt
     1741 aggacgcaac caccctcggg tcgtggacga cttcttctgc agtacctatc gcgacgacct
     1801 caccgaggtc gagcgccaac atccgatcgg tgatcgatgt gagcaacggt acgtcgtgtt
     1861 cgatgaccaa aaggctcgcc cctgtcatgg cccggatccg cagcaacaac gggccgagcg
     1921 cttcggcctc tcgctgtgcg atccctgagg atggctcgtc gagcaggagc acgcgcgggt
     1981 tgtgcgcgag cacacaggcg aggtcgacca ctcgccggct gccggtcgag agctcgcgga
     2041 tgtacttgtg gcggaaggcc tggagcccga gcagctcgat cagctcgtcg acccgggccc
     2101 gtttggcctt gccgcgcagg cgctgctcat aggcacggtc acacgcgacc gtgatcgtct
     2161 cggtcacggt cagcgcgggg aacagccggc cgtcctggaa ggagcgcccg aggccgcgcc
     2221 gggcgcgcgc gtcggggccg agccgcgcga tcgacgaacc ttcgagcaag acgttgccgc
     2281 ggtcggcgac gtggaaaccc gacaggacgt cgaacaatgt cgtcttgccc gcgccgttgg
     2341 gcccgaggaa cccgaggatc tcgccggctc gcacgtcgaa cgacacgtcg gtgagcgcgt
     2401 tgatgccgcc gaagcgcttg gagatgtcga acacttccag gcgcggcgcc gaggacccgt
     2461 tccccgacgg tgccgatgcg aggtcgacgt ctgcgtcgcg cacttcgatg gtcgggatcg
     2521 tcggcgcttg tgcttccttg tcggtgtcgt aggtcgccgc accctcgagg aagaccgagc
     2581 gcagcacgtc gggcc
//
LOCUS       DASUGA010000787         2281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1064102, whole genome shotgun sequence.
ACCESSION   DASUGA010000787 DASUGA010000000
VERSION     DASUGA010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064102"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VFX21_15455"
     CDS             complement(<1..344)
                     /locus_tag="VFX21_15455"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEX5097424.1"
                     /translation="MADEVAHVEREVALDIVKRGLIIAPIVIVFCGIIRGWSGAASAA
                     IGLGIVLLNFLAAAALMTRAAKSGPTAIGATALGGYIVRLAVVFVALLVLRNVSWIDM
                     PVLGFTIVGAQMG"
     gene            complement(329..556)
                     /locus_tag="VFX21_15460"
     CDS             complement(329..556)
                     /locus_tag="VFX21_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004409410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX5097425.1"
                     /translation="MWNGFSNALARAVELVGTVLVFVLIGLWIDSRLGTKPLFTVVLG
                     LLAILGQGIVAYYRYKAEVAAEEGERPWRTK"
     gene            complement(921..2114)
                     /locus_tag="VFX21_15465"
     CDS             complement(921..2114)
                     /locus_tag="VFX21_15465"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX5097426.1"
                     /translation="MGGYAIVLGAALAATFATTPLVRRLAIRFGAVVAPSSDARHVHT
                     RPTPTLGGAAMFVGFLVAMAVASQLDQFHEMFQANSEPFGLILGAGVIFLVGALDDLM
                     DVSPPAKVAGQVVAASLLALFGVTMFYFRMPFNLFHTDTVVLSSDLAPLVTAAWLVLM
                     CQAINLIDGIDGLAAGVVAIAGFALFLFADRLFKQNFIDGTNIGPLVAIIAVGVCLGF
                     LPFNWHPSKIIMGDAGALFLGLLLAVPTITIGGRTDHAFSGNTFFFFGPLLIPVLILG
                     VPILDTAFSFVRRVWNRQKWHQADAGHLHHRLMRLGHGPRRTVVILWAWTALLSGVAL
                     LPTYTNEGNALVPFAGAALLLLLMAWFHPGIRTRRERKERARHPTAHPTDDVVNLAER
                     RRQRA"
BASE COUNT          449 a          741 c          751 g          340 t
ORIGIN      
        1 cccatctgtg caccgacgat cgtgaagccg agaaccggca tgtcgatcca cgacacgttg
       61 cgcaacacca acagtgcgac gaacacgacc gcgaggcgga cgatgtagcc gccgagcgcc
      121 gttgcgccga tcgcagtcgg accactcttg gccgcgcgcg tcatgagcgc agcggcagcc
      181 aagaagttca acaacacgat gccgaggccg atcgcggcac ttgccgcgcc cgaccagccg
      241 cggatgatcc cgcagaaaac gatgacgatc ggggcgatga tgagaccgcg cttcacaatg
      301 tcgagcgcga cctcgcgttc tacatgcgct acttcgtccg ccacggcctc tccccttctt
      361 ccgccgccac ctcggccttg taccggtagt acgcgacgat cccctggccg agaatcgcga
      421 gcaggccgag gacaaccgtg aacaatggct tggtaccgag gcggctgtcg atccagaggc
      481 cgatcagcac gaagacaagg acggtgccga cgagttcgac cgctcgagcg agcgcattcg
      541 aaaacccgtt ccacatcgac tgctggccac gttcttggcc tgccggaagc accgcgaaga
      601 tccccttgcc ccacaaggct tgtgcccccg ccaccgttgc cgggaggccg cttgtgaacg
      661 cattcgcaat ctacgatttc gtcaacattg agtcaaaccg gagcggaaat attcggacta
      721 cctcgtaggt agccgcgagg gtcgcgggcg gacgcagccg ccccgacgcc ctcttttctc
      781 ccggactacc tcacgggtag tgatgcttct ttcaccgcgg aaaggccgaa gagaccgccg
      841 accccccacc agtcgaaagc agtacgccga aggcttgtcg tcggtgcgct cgcccgcccg
      901 cgtacctctt tgcgacggct ttacgcgcgt tggcggcggc gttcggcgag attcacgacg
      961 tcgtccgtcg gatgcgccgt gggatggcgc gcccgttcct tgcgttcacg gcgcgttcgg
     1021 attccggggt ggaaccacgc catgagcaag agcagcaatg ccgcgcccgc gaacggcacg
     1081 agcgcgttgc cctcgttcgt gtacgtcggc aacagcgcga cgcccgacag caacgcggtc
     1141 cacgcccaaa ggatgacaac ggtgcggcgg ggcccgtgac cgagccgcat gagccggtgg
     1201 tgcaagtggc cggcatcggc ctggtgccac ttctggcggt tccagacgcg gcgcacgaag
     1261 gagaacgcgg tgtcgaggat cgggacgccg agaatcagca ccgggatcag gagcggcccg
     1321 aagaagaaga acgtgttgcc ggagaaggcg tggtcggtgc gcccgccgat cgtgatcgtc
     1381 gggacggcga gcaagaggcc caggaacaac gcgcccgcgt cgcccataat tattttcgat
     1441 ggatgccagt tgaacggtaa gaacccgagg catacaccca ccgcgatgat cgcgacgagc
     1501 ggaccgatgt tggtgccgtc gatgaagttc tgtttgaaca agcgatcggc gaacaagaag
     1561 agtgcgaagc ccgcgatcgc gacgacgccg gccgcgaggc cgtcgatgcc gtcgatgagg
     1621 ttgatcgcct ggcacatcaa cacgagccac gcggcggtaa cgagcggcgc gagatcggag
     1681 ctcagcacga ccgtgtcggt gtggaacagg ttgaacggca tccggaagta gaacatcgtg
     1741 acgccgaaca gcgcgagcag tgatgcggcg acaacctgac ccgcgacctt cgcgggcggc
     1801 gacacgtcca tgaggtcgtc gagcgcgccg acgaggaaga tcacgcccgc gccgagaatc
     1861 aacccgaacg gttccgagtt ggcttggaac atctcgtgga actggtcgag ttgcgacgcg
     1921 acggccatcg cgacgaggaa cccgacgaac atcgccgctc cgcccagcgt gggcgtcggc
     1981 ctggtgtgca cgtggcgggc gtccgacgac ggcgccacga ccgcgccgaa ccgaatcgcg
     2041 agtcgccgaa cgagaggagt tgtcgcaaag gtcgccgcca gtgcggcgcc caggacgatc
     2101 gcgtaacccc ccatgcggcg gggctacagc tccggatagg gggtgaactt ggagcagagg
     2161 cggttcgcct gctccttgac gtccttggcc accgtctcgt cgtcgacggc gcgcagcgtc
     2221 gcgccgatga gggatgcgac ttcgcgcatc tcgtcggggc ccatgcccgc agtcgtgcag
     2281 g
//
LOCUS       DASUGA010000788         4997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1723803, whole genome shotgun sequence.
ACCESSION   DASUGA010000788 DASUGA010000000
VERSION     DASUGA010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1723803"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /locus_tag="VFX21_15470"
     CDS             <1..1302
                     /locus_tag="VFX21_15470"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX5097427.1"
                     /translation="LFDRARAQAAGALPDGPFRGVPIVVKDLDGSLEGAPLHMGNKLL
                     KDIGHTGTVTSYLFQKLQDAGFVIVGKTNTPEFGLLPVAEPEAHGPTRNPWNTDHSAG
                     GSSGGSASAVASLMVPAAHGGDGGGSIRIPASMCGLFGLKPSRGRVSLGPDENEGWAG
                     LVIRGSLTHTVRDSAAILDVIQGYMPGDPYTAPPPARLYRDEVGADPGRLRIALHTTT
                     PGELAVTDPECVAATEDAARLLESLGHTVERAAPPAFDEGEMLQSFTTILFASVAADL
                     ALLAKIAGREITAADVEPMTWFYNEAAEAVTAEEYVTALATAHDWSRRIVAWFFDAGY
                     DLMLTPTLAEPPPAIGDVASRADDPLRPATRALPFALYTGPFNMTGQPAMSVPTYWST
                     AGLPIGVQLVGRPNREDVLYRLAAQIEQARPWADRVPPLHA"
     gene            1295..2674
                     /locus_tag="VFX21_15475"
     CDS             1295..2674
                     /locus_tag="VFX21_15475"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEX5097428.1"
                     /translation="MPERSVRVAGGQGFYGDTPAAVDALLAEGVDYLCLEALAELTLA
                     ILQKDRQRDEARGYTRDLPAYLTRALPFVADGSTKIVTNAGGINPAAAARAAIDTAHS
                     LGITGIKIATVLGDDLMPRIAELRRDGVTFEHLDSGDPFDAMPAPPLFASAYLGARPI
                     ADALAQGADVVITGRVADASLFLAPLAYEHGWAWDDWDRLAAGIVVGHLLECSGQSLG
                     GNYSGDWWALPDPWLLPYPIATVAADGAATISKPAGSGGRVSTDTLRHQLLYEVHDPA
                     RYLAPDVVADFTSAQFEDLPDDRVRITGVRGAAATDTYKTLLAFHAGWSGEARMAFSW
                     PDAYEKAKATAAIFAKRVEMAGLAVDEWRIEYWGVDALGGPTVPDTPGCEPPECVLRV
                     AWRCADQRTAGLVGRELVPLTLSAPPAGMTGAGRASGGASELLGIWPTLVDKQLVDAH
                     VRVALEEVV"
     gene            2674..3060
                     /locus_tag="VFX21_15480"
     CDS             2674..3060
                     /locus_tag="VFX21_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097429.1"
                     /translation="MPRLALREICGYRSGDKGDIANVAIFADDDKTYALIVREVTADR
                     VKAHFGTMVQGRVDRYEAANVKALNFVMHGALGGGGPRSLRADSLGKTLGGALVRLEI
                     EVPEQLANRRRPRPDVSWAEEILGRL"
     gene            complement(3108..3938)
                     /locus_tag="VFX21_15485"
     CDS             complement(3108..3938)
                     /locus_tag="VFX21_15485"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX5097430.1"
                     /translation="MTTETNDLIAEVTQLPVQPGAAMRLLWMLEDPRTSAADLGRLIE
                     SDPSLSTQVIRLANTAFYGLSGKVSSAWRAVTVLGLATVRALATTAAFDLFSEKGRSV
                     PDDFWPHSVTSAAAAATLARRVGIQSNEAFSAGLLHDIGSALVFRRAPRRYDNVLERQ
                     TRDASLTLVEAEKAEFGVTHAEVGAAALSTMRFPADMVEAINQHHTDPAEVTSLLGRL
                     LVTADALAIAVDGITSEQNHEIGPCLESLDIPANSEQNLLEEIARDQENLAGFLTVKG
                     "
     gene            4113..>4997
                     /locus_tag="VFX21_15490"
     CDS             4113..>4997
                     /locus_tag="VFX21_15490"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX5097431.1"
                     /translation="MPRTVVPTRYDLELAPDLVTGRFTGTVAIALEYAEPTAEFALHA
                     TGLDVELRELRVRGARVPATITLDTERDRVVVRTGKPLGPGPATLTLDFGGTLSGSLV
                     GLYRSTFTDADGAAHTIAATQFEAPHARRAFPCFDEPEFKAVYAVTLVVADGLSALSN
                     GPEIAREPTGDGRVRVRFGETIAMSTYLVAFVIGELEVSDPVDAGGIPLRVVHVPGKA
                     HLGDFALGVGAFAVRFFQDYYGIPYPGEKLDLVALPDFAFGAMENLGCVTFRESLLLV
                     DEKNVTQQEAAQVALTVVH"
BASE COUNT          733 a         1786 c         1730 g          748 t
ORIGIN      
        1 ctgttcgacc gggcccgcgc gcaggcggcg ggcgcattgc ccgacggccc gttccgcggc
       61 gtgccgatcg tcgtgaagga cctcgacggc agtctcgagg gcgcgccgtt gcacatgggc
      121 aacaagctcc tgaaggacat cggtcacacc ggcacggtga cgagctacct gttccagaag
      181 ctccaggacg cgggcttcgt catcgtcggc aagacgaaca cgcccgagtt cggcctgcta
      241 cccgtcgcgg aaccggaggc gcacggcccg acccgcaacc cgtggaacac agatcacagc
      301 gcgggcggat cgagcggcgg gtccgcgtcc gcggtcgcga gcctcatggt gccggccgca
      361 cacggcggtg acggcggcgg ttcgatccgc atccccgcga gcatgtgcgg cctgttcggg
      421 ttgaagccgt cacgcgggcg cgtgtcgctc ggccccgacg agaacgaagg ctgggccggt
      481 ctcgtcatac gtggttcgtt gacgcacacc gtgcgcgaca gcgccgcgat cctcgacgtc
      541 atccagggct acatgccggg cgacccgtac accgcaccgc cgccggcgcg tctgtaccgc
      601 gacgaagtcg gcgccgatcc gggccggctg cgcatcgcgc tgcacacgac cacgcccggc
      661 gagctcgcag tcaccgatcc cgaatgcgtc gccgcgaccg aagacgcagc gcgtctgctg
      721 gagtcgctgg ggcacacggt cgagcgcgcg gcaccacccg cgttcgacga aggcgagatg
      781 ctgcagtcgt tcacgacgat cctgttcgcg tccgtcgcgg ccgacctcgc gctgctggca
      841 aagatcgccg gccgcgagat caccgccgcc gacgtcgagc ccatgacctg gttctacaac
      901 gaggcagccg aagccgtgac ggccgaggag tacgtcaccg cgctggcgac cgcgcacgac
      961 tggtcgcgcc gcatcgtcgc gtggttcttc gacgccggct acgacctcat gctcaccccg
     1021 acgctcgccg agccaccgcc cgcaatcggt gacgtcgcgt cgcgtgcgga cgatccactc
     1081 cgtccggcga cacgcgcgct gccgttcgcg ttgtacaccg gaccgttcaa catgaccgga
     1141 cagcccgcga tgtcggtgcc gacgtactgg tcgacagcgg ggttgccgat cggtgtgcag
     1201 ctggtcggcc ggccgaaccg cgaagacgtg ctgtaccgct tggcggcaca gatcgaacaa
     1261 gcgcggccgt gggcggatcg ggtcccgccg cttcatgcct gagcgatccg ttcgcgtcgc
     1321 gggcgggcag ggtttctacg gcgacacgcc ggccgcggtc gacgcgttgc tcgcggaggg
     1381 cgtcgactac ctgtgcctcg aagcgctcgc ggagttgacg ctcgcgatct tgcagaagga
     1441 ccgccagcgc gacgaggcgc gcggctacac ccgagacctg cccgcgtatc tcacgcgcgc
     1501 gctcccgttc gtggcggacg gcagcacgaa gatcgtgacg aacgcgggcg gcatcaaccc
     1561 ggccgccgcg gcccgggccg cgatcgacac cgcgcactcc ctcggtatca ccggcatcaa
     1621 gatcgcaacg gtgctcggtg acgatctcat gccgcgcatc gccgagctcc ggcgcgacgg
     1681 cgtgactttc gagcatctcg acagtggcga tccgttcgac gcgatgcccg cgccgccgtt
     1741 gttcgcgtcc gcgtacctcg gtgcgcgccc cattgccgac gcactcgccc agggtgccga
     1801 cgtcgtcatc accggccgcg tcgccgacgc gtcactgttc ctcgcgccgc tcgcctacga
     1861 gcacggatgg gcgtgggacg actgggaccg gctcgcggcc ggcatcgtcg tcggccacct
     1921 cctcgagtgc tcggggcaga gcctgggcgg caactactcc ggcgactggt gggcgttgcc
     1981 cgacccgtgg ttgttgccgt acccgatcgc caccgtcgcc gccgacggcg cggcgacgat
     2041 ctcgaagccg gcgggttcgg gcgggcgcgt gagcaccgac acactgcgcc accagctcct
     2101 gtacgaggtg cacgatccgg cgcgctatct cgcgcccgac gtcgtcgccg acttcaccag
     2161 cgcgcagttc gaagacctgc ccgacgaccg cgtgcgcatc accggcgtcc gcggcgcggc
     2221 ggcgaccgat acctacaaga cgctgcttgc gttccacgcg ggttggtcgg gcgaggcgcg
     2281 catggcgttc tcgtggccgg acgcgtacga gaaggcgaag gcgacggccg cgatcttcgc
     2341 caagcgggtc gagatggcgg gcctggcggt cgacgagtgg cgcatcgagt actggggcgt
     2401 cgacgcgctc ggcggcccga ccgtgcccga cacacccggc tgcgaaccgc cggaatgcgt
     2461 gctgcgcgtc gcgtggcgct gtgccgacca gcgcaccgcc gggctcgtgg gccgggaact
     2521 ggtgccgttg acgttgtcgg caccacccgc gggcatgacc ggcgcgggcc gggcgagcgg
     2581 cggcgcgagc gagctgctcg gcatctggcc gaccctcgtc gacaagcaac tcgtcgacgc
     2641 gcacgtgcgt gtcgcgctgg aagaagtggt gtgatgcctc gcctcgcgct gcgggagatc
     2701 tgcggctacc gatcgggcga caagggcgac atcgcgaacg tcgcgatctt cgccgacgac
     2761 gacaagacgt acgcgctgat cgtgcgcgag gtcaccgccg atcgggtgaa ggcacacttc
     2821 ggcacgatgg tgcagggccg ggtcgatcgc tacgaagccg cgaacgtcaa ggcgttgaac
     2881 ttcgtgatgc acggcgcgct cggcggcggc ggcccgcggt cattgcgcgc cgacagcctc
     2941 ggcaagacgc tcggcggcgc gctcgtccgc ctggagatag aagtgcctga acaactggcg
     3001 aaccgccgac gcccccgccc cgacgtgagt tgggcagaag agatcctcgg gcgtctctga
     3061 agggcggaat tcgcccctgt aagacgcccg accgtcgttg ctgggtctca gcccttgacg
     3121 gtgaggaaac cggccaggtt ctcctggtcg cgggcgatct cctcgagcag gttctgctcg
     3181 gagttcgcgg ggatgtcgag cgactcgagg cagggcccga tctcgtggtt ctgctccgac
     3241 gtgatgccgt cgaccgcgat cgccagcgcg tccgccgtga cgagcagccg cccgagcagc
     3301 gacgtcactt ccgcggggtc ggtgtgatgc tggttgatcg cttcgaccat gtccgcgggg
     3361 aagcgcatcg tcgagagtgc agccgcaccc acctcggcgt gggtcacgcc gaactccgcc
     3421 ttctcggctt cgaccagtgt gagcgacgcg tcgcgagtct ggcgctcgag cacgttgtcg
     3481 tagcggcggg gcgcccggcg gaacaccagc gccgaaccga tgtcgtgcag caagccggcg
     3541 ctgaatgcct cgttgctctg gatgccgacc cggcgcgcca gcgtggccgc ggccgccgcc
     3601 gaggtgacgg agtgcggcca gaagtcgtcg ggcaccgagc ggcccttctc cgagaagagg
     3661 tcgaacgccg cggtggtcgc cagggcccgc accgttgcga ggccgagcac ggtgacggcg
     3721 cgccacgcgc tcgacacctt gccggagagt ccgtagaacg cggtgttggc gagccggatg
     3781 acctgagtcg acagcgacgg gtccgactcg atgagccggc cgaggtcggc cgcagacgtc
     3841 cgcgggtcct cgagcatcca gaggagccgc atggccgcgc cgggctgcac cggcagctgg
     3901 gtgacttctg cgatcagatc gttcgtctcg gtcgtcattc gcgcgctccc gattgcccgc
     3961 gtcgggcctt cacctgtccc ctgttatcgg cgttcgggcc ccgcgcctga gggacaagta
     4021 atcgcgaccc aaacggggca gcgggccgcc ggtccgccta cgctgcgtgg ctccgggcga
     4081 ccggccccct gcctatgacc tcaccgcacc gcttgccccg caccgtcgtc ccgacgcgtt
     4141 acgacctcga gctcgcgccc gacctcgtga cgggccggtt cacgggcacc gtcgccatcg
     4201 ccctcgagta cgccgagccg accgccgagt tcgcgctgca cgccacgggc ctcgacgtcg
     4261 aactgcgcga actacgtgtt cgcggcgccc gcgtccccgc gacgatcacc ctcgacaccg
     4321 agcgtgatcg cgtcgtcgtg cgcacgggca aaccactcgg gcccggcccg gcgacgctca
     4381 cgctcgactt cggggggacg ctctccggca gcctcgtcgg cttgtaccgc tcgacgttca
     4441 ccgacgccga cggcgcggcg cacacgatcg ccgccacgca gttcgaagcg ccgcacgcgc
     4501 gccgcgcgtt cccgtgcttc gacgaacccg agttcaaggc cgtctacgca gtcacgctcg
     4561 tcgttgccga cggcctgagc gcactgtcga acggcccgga gatcgcgcgc gaacccaccg
     4621 gcgacggtcg cgtccgcgtg cgcttcggcg agacgatcgc gatgtcgacg tatctcgtcg
     4681 cgttcgtgat cggcgagctc gaagtgtccg atccggtcga tgcgggcggc attccgctgc
     4741 gcgtcgtgca cgttcccggc aaggcacacc tcggagactt cgcgctcggg gtcggcgcct
     4801 tcgcggtgcg cttctttcag gactactacg gcattccgta tccgggcgag aagctcgacc
     4861 tcgtcgccct ccccgacttc gcgttcggcg cgatggagaa cctcgggtgc gtgacgttcc
     4921 gcgagtcgtt gttgctcgtc gacgagaaga acgtcacgca acaggaagcc gcgcaggtcg
     4981 cgctcaccgt cgtccac
//
LOCUS       DASUGA010000789         3030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1185752, whole genome shotgun sequence.
ACCESSION   DASUGA010000789 DASUGA010000000
VERSION     DASUGA010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185752"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VFX21_15495"
     CDS             complement(<1..438)
                     /locus_tag="VFX21_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097432.1"
                     /translation="MRWRLAAVLLILGLGAAAVTACSGDDDDSGSTTTVSDPGPNSTI
                     SDPGPNDTSSGPSTIVGIVAFTTSDGCITLTATQPFSLRFDGFTLQNDEDANLALVAD
                     DDGRVLSHNGDQMFVTGRENGETDACGTVFVVESLNSVVPTATT"
     gene            472..885
                     /gene="arfB"
                     /locus_tag="VFX21_15500"
     CDS             472..885
                     /gene="arfB"
                     /locus_tag="VFX21_15500"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017792122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX5097433.1"
                     /translation="MDVLRVTRSCAIPLDELDWRFTASGGPGGQHANTSNTKVEVRFD
                     IASSPALGPRQRARLLERLGPVARASASERRSQLQNRELALDRLARKLANALHVDAPR
                     TATKPSRAAKKARLDDKRKQSDRKRARRRPSRGDD"
     gene            complement(1885..>3030)
                     /locus_tag="VFX21_15505"
     CDS             complement(1885..>3030)
                     /locus_tag="VFX21_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005457647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5097434.1"
                     /translation="GHWYGWLGLAITCVSIYGLSRCVQAARATRGEFASALAGSLGPE
                     WAADLDPRWKRQDERLEWRRVATPFRFKRRGVERIRNIQYVDGDHIRRHRLDIYRSPD
                     AGPGAPVLLQIHGGAWVIGNKEQQGLQLMNLLATRGWVCVAINYRLSPHYTWPDHIVD
                     CKLAMKWIRAHIAEYGGDPDYVVATGGSAGGHLTALLGLTANDPQFQPGFEAVDTSVR
                     AMVPFYGVFDWTNRFGQRGKKDGLRSTVLERLVVKKKFEDAREVFDQASPMSHVRPDA
                     PPTLVVHGDSDTLAPVAEAREFVRMLRDVSHNPVVYVELKGAHHAFEIIQSIRSLETI
                     AAVDEFLSWLLHHDAPRAVAELASPPASPGPTASAASDRTTTAHTAP"
BASE COUNT          445 a         1023 c         1051 g          511 t
ORIGIN      
        1 ggttgttgcg gtcgggacga ccgagttcag gctctcgacg acgaagaccg tgccgcaggc
       61 gtcggtttcg ccgttctcgc ggccggtgac gaacatctgg tcgccgttgt gggagagcac
      121 acggccgtcg tcgtcggcga cgagcgccag gttggcgtct tcgtcgttct ggagcgtgaa
      181 gccgtcgaag cggagcgaga acggttgcgt cgccgtcagc gtgatgcagc cgtcggatgt
      241 cgtgaaggcg acgatgccca cgatggtcga cggtcccgac gacgtgtcgt tgggaccagg
      301 gtcactgatg gtcgagttgg gtccggggtc ggacacggtc gtggtcgaac cgctgtcgtc
      361 gtcgtcaccg ctgcacgcgg tcacggccgc cgcgcccaac ccgagaatca gcagcaccgc
      421 cgccagtcgc catcgcaccg ggagagtctg cctccgccgt aacctcgaac ggtggacgtg
      481 ctccgcgtga cgcgcagctg tgcgatcccg ctcgacgagc tggactggcg gttcaccgcg
      541 tccgggggac ccggtgggca gcacgcgaac acgtccaaca ccaaggtcga ggtgcgcttc
      601 gacatcgcga gctcgcccgc gctcggcccg cgccagcggg cccgcctgct cgagcggttg
      661 gggccggtcg cgcgcgccag tgcatccgaa cggcgttcgc aactgcagaa ccgtgagctc
      721 gcgctcgacc gtctcgcccg caagctcgcg aatgcgctgc acgtcgacgc gccccgcacc
      781 gcgacgaagc cgagccgcgc cgccaagaag gcgcgcctcg acgacaagcg aaagcagtcc
      841 gaccgcaagc gggcgcggcg ccgaccgagc cgcggcgacg attgatgacg ctgttcgacg
      901 tggtcctgct cgtcgccggt gtcgccgcgg ttatctgcgt actcgacgcg gtgatccgca
      961 cgttcgtcgt cccgcgcggg cagcaggtca cgttcacctt cgtcgtgttc cgcggcgtgc
     1021 gatgggtgat gcagctcgtt gcgcgcccac agtcgagcta cgaacgccgc gaccgcgtga
     1081 tggcgttcta cgcaccgctc gccctcctca cgctgcctgc ggtcttcctc ttcgttgtct
     1141 tcatggcctt cagcctgatc ttctgggcga cgacgcacga ccagtggcag agcgcggccg
     1201 aggagagcgg gtcatcactg ttgacgctcg gtttccagcg tcccgacgac ttcgcgccca
     1261 cgctggtcgt cttcctggaa gccgcgatcg gcctcggcct cctggcgatc ttgatcgcct
     1321 acctgcccac gatctacggc gcgttctcgc ggcgagagat gctcgtcaac gagctgaccg
     1381 tgcgcgcggg aacgccgccg aaaggatggg agatcctcga acgcggccag gccgcgggct
     1441 acttgatgaa cctcgatccc ttcttcgagc agtggatgtc gtggttcgca gaactgtcgg
     1501 agacgcacac gtcgctcggc tcgctcgcgt tcttccgttc gccgaacgcc aatcagtcgt
     1561 ggatcaccgc gagtggcgca gtgctcgacg cggcatcgtt gcggatggcg gtgctcgaca
     1621 tcccgttcac ggccgccggc ggtctctgca ttcgctccgg cttcctcgcc ctgcgggaga
     1681 tcgcgggcta cttcggcttc gagtacgacc ccgatccgcg cgccgacgat ccgatctgca
     1741 tctcgcgcga agagttcgac gcggtctacg acaagctcgt cgcgaacgaa gtgccggtga
     1801 aggccgaccg cgaccaggcg tggcgcgact tcgcgggctg gcgagtgaac tacgactcgg
     1861 tgctgatcac cctggccggg ttcctcatgg cgccgtatgc gccgtggtcg tccgatcgct
     1921 cgccgcagac gccgttggcc cgggtgacgc cggtggggaa gcgagctcgg caactgcgcg
     1981 cggggcgtca tggtgcagga gccagctgag gaactcgtcg acggccgcga tcgtctcgag
     2041 gctgcggatc gactggatga tctcgaacgc gtggtgcgcg cccttcagct ccacgtagac
     2101 gaccgggttg tggctcacgt cgcgcagcat gcgcacgaac tcgcgcgctt cggcgaccgg
     2161 cgcgagggtg tcgctgtcgc cgtggacgac cagcgtcggc ggcgcgtcgg gccggacgtg
     2221 cgacatcggt gaggcctggt cgaagacctc gcgggcatcc tcgaacttct tcttgacgac
     2281 gagtcgttcc aggacggtgc tgcgcaggcc gtccttcttg ccgcgctgcc cgaaccggtt
     2341 ggtccagtcg aacacgccgt agaacgggac catcgcgcgc acgctcgtgt cgacggcttc
     2401 gaacccgggc tggaactgcg ggtcgttcgc ggtcaggccc aggagcgcgg tgagatgacc
     2461 gcccgcggat ccgcccgtgg cgacgacgta gtcgggatcg ccgccgtact cggcgatgtg
     2521 cgcacggatc cacttcatcg ccagcttgca gtcgacgatg tggtcgggcc aggtgtagtg
     2581 cggcgacagg cggtagttga tcgcgacgca gacccagccg cgcgtcgcga gcaggttcat
     2641 caactggagc ccctgctgct ccttgttgcc gatgacccat gcgccaccgt ggatctgcag
     2701 cagcaccggt gcgccgggac ccgcgtcggg ggagcggtag atgtcgagcc ggtggcgccg
     2761 gatgtggtcg ccgtcgacgt actgaatgtt gcggatgcgt tcgacgccgc ggcgcttgaa
     2821 ccggaacggc gtcgcgacgc gccgccactc gagccgttcg tcttggcgct tccaacgcgg
     2881 atcgaggtcg gcggcccatt ccggtccgag cgacccggcg agcgccgacg cgaactcgcc
     2941 gcgcgtcgcg cgtgcggcct gcacacaccg cgagaggccg tagatgctga cgcacgtgat
     3001 cgcgaggccg agccacccgt accagtgacc
//
LOCUS       DASUGA010000790         7105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1481586, whole genome shotgun sequence.
ACCESSION   DASUGA010000790 DASUGA010000000
VERSION     DASUGA010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7105
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481586"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="VFX21_15510"
     CDS             <1..1352
                     /locus_tag="VFX21_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosamine-6-phosphate synthase"
                     /protein_id="HEX5097435.1"
                     /translation="RHPLQRRSWAIIGNGVNRVAAEEIRIKLSELCYKSISCDSTEDK
                     KHIDLSAEPMIFVCAAGLRGSNADDVGKELAIFRAHKAAAIVVADDDETRLSAALETI
                     FVPVVHTDLAFVLSTMVGHLFGYEAALAIDATARPLREARGRIEAIVAAASEDGDMQR
                     VLARELEPHAEAFFGLLRTGSYDGAMEAGTAVRLASLLRYATGVVPLDAYQVEFGKVG
                     TPSTVVEDLTAALTKGVEELTRPIDAIKHQAKTVTVGISRSDETLLDVELVQELLATG
                     VARDSLTYRALRTVVSLDPVVDDVVGWTRYRIEGDPLNGSATIQVIDSGGIARDIPSR
                     TATNPTLRGTKHRAASEREVTVVRGRADGRTLILVPEVKANQTIGLTLLHVRFADRLP
                     AETARRVLEGYRDRYNAIADQVTEVEPVMRDDVLATIDMVELLTEPVVRLADRWKTG"
     gene            1360..2793
                     /locus_tag="VFX21_15515"
     CDS             1360..2793
                     /locus_tag="VFX21_15515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX5097436.1"
                     /translation="MKPVLTPAEMAEADRRAIAGGTPEAVLVERAGRAVARHALAILG
                     GAYGRRVVVVSGKGNNGADGLVAARVLAARGVGVDVFRLADGIDGVDRAVGRADLAID
                     AMFGTGFRGALDGDAARAADAIRSVRTLAVDIPSGVNGTTGEVSGVAVTAQETTCFVA
                     AKPGLLFEPGRSHAGRVRVVDIGTDPGAATIAVPELRDLRLPSRGATDHKWTSGLFVF
                     GGSKGMLGAPAMTGRAAARSGAAIVVCGVPAAGLGASEIITRALPATSDGHLDEDAAR
                     LVVKELHRYRALAIGPGLGRDDRAQAAARRLVAEAALPVVIDADALNALATDTSPLRV
                     RHAQKVPRAVLTPHAAEYERLAGHPVGADRVGAARDLAAQTQAVVVLKGPGTVIAEPA
                     GRAVVNPTDTAALATAGTGDVLTGIIGGLLANGADPFAAAVTGAYVHGRAARVAGTGD
                     DLVAIDLIAALHRTLDALRQGHDPGED"
     gene            2794..3264
                     /locus_tag="VFX21_15520"
     CDS             2794..3264
                     /locus_tag="VFX21_15520"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5097437.1"
                     /translation="MPADTLVRDVMSTNVSTLRGETPITEAADTMAASGFGAMPVVDE
                     KGHLLGLLRDDDLLATEARVHVPTFFNFFDATIPLPGEMHHLKKELERIAGSTVADVM
                     DQDPLTIGPDETLEELATRMHDAEISHVPVVDADGKVVGVVARGDIVKFIARTT"
     gene            3261..4391
                     /gene="alr"
                     /locus_tag="VFX21_15525"
     CDS             3261..4391
                     /gene="alr"
                     /locus_tag="VFX21_15525"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX5097438.1"
                     /translation="MTATRYRPVWAEVDLGAVRDNVRALAAVVAPARVMAVVKADGYG
                     HGAIPVSDAALDAGAAQLGVALVEEGVRLRETGIDAPVLVLSEPVADAAETVVRNDLV
                     PVVYTVPGVEALAKAVVAVGGTEPLPVHLKVDTGMHRVGCRPEDALDLALHIQSHPEL
                     RLAGTCTHLAVADEPGNEYSNDQLARFALLLDTLRANRVEPGIVHTANTAGALALPAA
                     RYDLVRAGIGIYGIHPSDELATSVDLRPALAVKAQVSFVKDLPAGARISYGLRYELPH
                     ASRVATVPIGYADGVPRALGHRDGEVLIRCHRCRIAGTITMDQLMVDVGDAPVEAGDE
                     VVLLGAQGSEQITAGDWARKLDTIPYEVVCQIGPRVPRRYIG"
     gene            4388..5371
                     /locus_tag="VFX21_15530"
     CDS             4388..5371
                     /locus_tag="VFX21_15530"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5097439.1"
                     /translation="MNGRSVVKGATATAGIVGGVVGAAWATQRLAARRIRQRPDDDAK
                     RSLEEPIYVDEMLPTHDGGTLYVVEDGTGPAVVLSHGVTLSVRTWFHQLELLPKEGLR
                     AIAFDHRGHGQSVLGDDGYSIDNLATDLKTLIEALDLRDVVLVGHSLGGVAVQAFFLQ
                     FPDLARERVKGIVLLSTLAKAPLGSQSTRYKARIERIFKRVPDTTRFWASPNFGLAVA
                     RLGFGTDPKPSHVELVRRMMAACSHDTRVHAPRMLVGLDLTDSLPKIDVPTLVIGGTA
                     DIVTPPAEAERMAALIPNAQLVLLDGGGHMLMLEQAEEVSALIAGFTRQLG"
     gene            5368..6258
                     /locus_tag="VFX21_15535"
     CDS             5368..6258
                     /locus_tag="VFX21_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020521964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX5097440.1"
                     /translation="MNILAGVRGGHWTGAGTGVTVLLVPPDTVGAGEVRGGAPATREF
                     ALLDPLRTVSRVDAVVLTGGSAFGLATADGVMRYLAERGQGYPTAGGPVPIVPTAAVF
                     DLVHAGVRPPGATEGYAAATMAARDAAFATGRVGAGRGVTVGKWRGREYAVPGGVGLA
                     RRRVDGATVAAVVVVNGVGDVVGEDGSLLAGSTAPDEAESFATPRPFEETATGENTTL
                     VVVVTDGRVDKVGAALVAQSAHDGIARALRPAHTRFDGDASIALGTGACDVHLDRLRA
                     ETVEVVAAAIRDAVTTPGDG"
     gene            6282..6905
                     /locus_tag="VFX21_15540"
     CDS             6282..6905
                     /locus_tag="VFX21_15540"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX5097441.1"
                     /translation="MSSDPDLLHADHPANATGASLAQVAIASHDCNLCKLAGGRTQVV
                     FGTGREDADLMFVGEGPGEQEDIQGIPFVGRAGQLLTQLIEGIGLTRDDVYIANVVKC
                     RPPGNRDPEPEEIEACAPWLDRQLALIRPTVIVTLGNFATKLLLGTKEGITKLRGNEF
                     AFSRAGIDAALFPTLHPSAVLRSGGGQKLAEVRSDFVQVKRALVRGA"
     gene            6902..>7105
                     /gene="tsaE"
                     /locus_tag="VFX21_15545"
     CDS             6902..>7105
                     /gene="tsaE"
                     /locus_tag="VFX21_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX5097442.1"
                     /translation="MKTTTTSAEHTRALAAAIGAQLDAGDVVVLVGEMGAGKTTFVQG
                     LARGLGVDEIVTSPTFTIVQEYDG"
BASE COUNT         1030 a         2491 c         2547 g         1037 t
ORIGIN      
        1 aacggcatcc gttgcagcgg cggtcgtggg cgatcatcgg gaacggcgtc aaccgcgtcg
       61 cggccgagga gattcgcatc aagctctcgg agctctgtta caagtcgatc tcctgcgact
      121 cgacggaaga caagaagcac atcgaccttt ccgccgaacc gatgatcttc gtgtgcgccg
      181 cgggattgcg gggctcgaac gcggacgacg tcggcaagga gctcgcgatc ttccgcgccc
      241 acaaggccgc ggcgatcgtc gtcgccgacg acgacgagac gcggttgtcg gccgcactgg
      301 agacgatctt cgtgcccgtc gtccacacgg atcttgcgtt cgtgctgtcg acgatggtcg
      361 gtcacctctt cggctacgag gccgcgctcg ccatcgacgc gaccgcgcgc ccgttgcgcg
      421 aagcgcgcgg ccggatcgaa gcgatcgtcg cggccgcgtc cgaggacggc gacatgcaac
      481 gtgtgctcgc gcgcgagctc gaacctcacg ccgaggcgtt cttcggcctg ctgcgcaccg
      541 gctcctacga cggcgcgatg gaggccggca ctgccgtacg gcttgcgtcg ctcctgcgct
      601 acgcgaccgg ggtcgtgccg ctcgacgcgt accaagtcga gttcggcaag gtcggcacgc
      661 cgagcaccgt cgtcgaggat ctgaccgcgg cgctcacgaa aggcgtcgag gagctgacgc
      721 ggccgatcga cgcgatcaag caccaggcga agaccgtgac cgtcggcatc tcgcgctccg
      781 acgagaccct gctcgacgtc gagttggtgc aggagctgct cgcgactggc gttgcacgcg
      841 acagcctcac ctaccgcgcg ctgcgcaccg tggtttcgct cgaccccgtc gtcgacgacg
      901 tcgtcggctg gacgcgctac cgcatcgagg gtgacccgct gaatggaagc gcgacgatcc
      961 aggtcatcga ctcgggtggc atcgcgcgcg acatcccgag ccgtactgcc acgaacccga
     1021 cgctgcgagg gacgaagcac cgcgccgcga gcgaacgcga ggtcacggtg gtgcgcgggc
     1081 gcgccgacgg ccgcacgctg atcctcgtcc ccgaggtcaa ggccaaccag acgatcgggc
     1141 tcacgctgtt gcacgtgcgg ttcgcggacc ggctgcccgc ggagaccgcg cgtcgcgtgc
     1201 tcgagggcta ccgcgaccgt tacaacgcga tcgccgacca ggtcaccgag gtcgaaccgg
     1261 tcatgcgcga cgacgtcctc gcgacgatcg acatggtcga gttgttgacc gagccggtgg
     1321 ttcgcctcgc cgaccgctgg aagaccgggt agccctcggg tgaaacccgt gctgacgccc
     1381 gccgagatgg ccgaggccga ccggcgcgcg atcgcgggcg gcacgccgga ggccgtgctc
     1441 gtcgagcggg ccggacgcgc ggtggcgcgg cacgcgttgg cgatcctcgg tggcgcgtac
     1501 ggccgccggg tcgtggtggt gagcggcaag ggcaacaacg gcgccgacgg tctggtcgcc
     1561 gcccgcgtgc tcgcggcgcg cggtgtcggt gtcgacgtgt tccggctcgc ggacggtatc
     1621 gacggcgtcg accgggccgt cggacgagcc gacctcgcga tcgacgccat gttcggcacc
     1681 ggctttcggg gcgcgctcga cggcgacgcg gcgcgcgccg ccgacgcgat ccgctcggtc
     1741 cgcacgctgg cggtcgacat cccatcgggc gtgaacggca cgacgggcga ggtgtcgggt
     1801 gtcgcggtca ccgcacagga gacgacgtgc ttcgtcgccg cgaaaccggg gctgttgttc
     1861 gagcccggcc gatcgcacgc cggacgcgta cgcgtcgtcg acatcggcac cgatcccggt
     1921 gcggcgacga tcgcagtccc ggagctgcgc gatctgcgct tgccgtcacg cggtgcgacc
     1981 gaccacaagt ggacgtcggg gttgttcgtg ttcggcggtt cgaagggcat gctcggcgcg
     2041 ccggcgatga ccggccgcgc cgcggcccgc agcggcgccg cgatcgtcgt gtgcggtgtc
     2101 ccggctgccg gcctgggcgc gtccgagatc atcacgcggg cgttaccggc gacgagcgac
     2161 ggccacctcg acgaggacgc ggcccggctc gtcgtcaagg aactgcaccg ttaccgcgcg
     2221 ctcgcgatcg gtcccggcct cggccgcgac gatcgcgccc aggccgccgc gcgccggctc
     2281 gtcgcggagg ccgcgttgcc ggtcgtgatc gacgccgatg cgttgaacgc gctcgcgacg
     2341 gatacctcac cgctgcgggt gcggcacgcg cagaaggtgc cgcgcgcggt cctcacgccg
     2401 cacgccgcag agtacgagcg cctcgcgggc catcccgtcg gcgccgaccg ggtcggtgcg
     2461 gctcgggacc tcgcggcgca gacccaggcc gtcgtcgtgt tgaaggggcc cgggaccgtc
     2521 attgcggagc ccgccggccg cgcggtcgtc aaccccactg acacggccgc gctcgccacc
     2581 gccgggaccg gcgacgtcct caccggcatc atcggtgggc tgctcgccaa cggtgcggac
     2641 ccgttcgcgg ccgcggtgac cggcgcctac gtccacggcc gggccgcgcg ggtagccggc
     2701 accggtgacg atctcgtcgc gatcgacctg atcgcggcgt tgcaccgtac gctggatgca
     2761 ctgcggcagg gccacgatcc cggggaggac tgaatgccgg cggacactct cgtgcgtgac
     2821 gtgatgagca ccaacgtctc gacactgcgc ggcgagaccc ctatcaccga agcggccgac
     2881 acgatggccg cgtcgggttt cggtgcgatg ccggtcgtcg acgagaaggg tcacctgctc
     2941 gggctgctgc gcgacgacga cctccttgcg accgaggcgc gggtgcacgt gccgacgttc
     3001 ttcaacttct tcgacgcgac gattccgctc ccgggtgaga tgcaccacct caagaaggag
     3061 ttggagcgca tcgcgggctc gaccgtcgcc gacgtgatgg atcaagaccc gctgacgatc
     3121 gggcccgacg agacgctcga agagctggcg acgcgcatgc acgacgccga gatctcgcac
     3181 gtaccggtgg tcgacgccga cggcaaggtc gtcggcgtcg tcgcgcgcgg cgacatcgtg
     3241 aagttcatcg cgcgcaccac atgaccgcga cgcgctaccg gcccgtatgg gccgaggtag
     3301 acctgggcgc cgtgcgcgac aacgtgcgcg cgctcgctgc cgtcgtcgca cccgcgcgcg
     3361 tgatggcggt cgtcaaggcc gatggttacg gccatggcgc gatccccgtg agcgacgccg
     3421 cgctcgacgc cggtgctgcg cagctcggtg tcgcgctcgt cgaagaaggc gtgcgcctgc
     3481 gcgagaccgg catcgacgcg ccggtcctcg tgctgtcgga gccggtggcg gacgcggccg
     3541 agaccgtcgt gcgcaacgat ctcgtgcctg tggtctacac ggtgccgggc gtcgaggcgc
     3601 tggcgaaagc cgtcgtcgcc gtgggcggca ccgagccgtt gccggtgcac ctcaaggtcg
     3661 ataccggcat gcaccgcgtc ggctgccggc ccgaagatgc tctcgacctc gcgctgcaca
     3721 tccaatcgca cccggagctg cggctcgcgg gcacgtgcac gcatctcgcg gtcgccgacg
     3781 agcccggcaa cgagtactcg aacgatcagt tggcgcgctt cgcgctcctg ctcgacacgc
     3841 tgcgcgccaa ccgcgtcgag ccgggcatcg tgcacacggc caacaccgcc ggcgcgctcg
     3901 cgctccccgc cgcgcgctac gacctcgtgc gcgccggcat cgggatctac ggcattcatc
     3961 cttccgacga actggcgaca tcggtcgacc tgcgtccggc gttggcggtc aaggcgcaag
     4021 tgtcgttcgt gaaggacctc ccggccggcg cgcgcatctc gtacggcctg cgttacgagt
     4081 tgccgcacgc gagtcgcgtc gcgacggtac cgatcggtta cgcggacggt gtgccgcgcg
     4141 cgctcggtca tcgcgacggc gaagtgctga tccgctgcca ccggtgccgg atcgcgggga
     4201 cgatcaccat ggaccagctc atggtcgacg tcggcgacgc gcccgtcgag gccggtgacg
     4261 aggtcgtgtt gctcggcgcg cagggcagtg agcagatcac ggccggcgac tgggcccgca
     4321 agctcgacac gattccctac gaggtcgtgt gccagatcgg cccccgagtc ccgaggcggt
     4381 acatcggatg aacgggcgca gcgtcgtcaa gggcgcgacc gccaccgcgg gcatcgtcgg
     4441 tggtgtggtc ggtgcggcgt gggcaacgca acggctcgcg gcgcgacgga tccgtcagcg
     4501 cccggacgac gacgcgaagc gttcgctcga agagccgatc tacgtcgacg agatgttgcc
     4561 cacgcacgac ggcggcacgc tttacgtcgt cgaggacggc accggccccg cggtcgtgct
     4621 gtcgcacggc gtcacgttgt ccgtgcggac gtggttccac cagctcgagc tcttgccgaa
     4681 ggaaggtctg cgcgcgatcg cgttcgacca tcgcggccac ggccagtcgg tgctcggcga
     4741 cgacggctac tcgatcgaca atctcgcgac cgatctcaag acgctgatcg aagcgctcga
     4801 cctgcgcgat gtcgtcctcg tcgggcactc gctcggcggt gtcgcggtgc aggcattctt
     4861 cctgcagttc cccgacctcg cgcgggagcg agtgaagggc atcgtgctcc tgtcgacgct
     4921 ggcgaaggcg ccgctcggtt cgcagtcgac gcgctacaag gcccgcatcg aacgcatctt
     4981 caagcgtgtt cccgacacaa cgcgcttctg ggcgtcgccc aacttcggcc tcgcggtggc
     5041 gcgcctcggt ttcggcaccg atccgaagcc gagccacgtc gagctggtgc ggcgcatgat
     5101 ggctgcctgc tcgcacgaca cgcgtgtgca cgcgccgcgc atgctcgtcg ggctcgacct
     5161 caccgacagc ctgcccaaga tcgacgtgcc gacgctcgtc atcggtggca ccgccgacat
     5221 cgtgacgcca ccggccgaag ccgaacggat ggcggcgctg atcccgaacg cgcagctggt
     5281 gctgctcgac ggcggcgggc acatgctgat gctggagcag gcggaggaag tctccgctct
     5341 catcgccggg ttcacacgtc aactcgggtg aacattcttg ccggcgtgcg cggcggccac
     5401 tggacggggg ccggaaccgg tgtgaccgtc ttgctcgtcc cgcccgacac cgtgggcgcg
     5461 ggcgaagtgc gcggcggcgc gcccgcgaca cgcgagttcg cgttgctgga tccgctgcgc
     5521 acggtctcgc gcgtcgatgc ggtcgtgctg acgggtggtt ccgcgttcgg tctcgcgacc
     5581 gccgacggcg tgatgcgcta cctcgcggag cgcgggcagg gctacccgac cgcgggcggc
     5641 ccggtgccga tcgtcccgac ggcggcggtc ttcgacctcg tgcacgccgg cgtgcgcccg
     5701 cccggcgcga ccgagggcta cgcggccgcg acgatggccg cgcgcgacgc ggcgttcgcg
     5761 accggccggg tcggcgccgg ccgcggcgtg accgtcggga agtggcgcgg tcgcgaatac
     5821 gccgtcccgg gcggtgtcgg gctggcccgc cgccgggtcg acggcgcaac cgtggccgcg
     5881 gtcgtggtcg tgaacggcgt cggcgacgtc gtcggcgagg acggcagctt gctcgcgggc
     5941 tcgaccgctc cggacgaggc ggagtcgttc gccacgccac gaccgttcga ggagacggcc
     6001 accggcgaga acacgacgct cgtggtcgtc gtgaccgacg gtcgcgtcga caaagtcggc
     6061 gccgcgctcg tcgcgcagag cgcgcacgac gggatcgcgc gggcactgcg tccggcccac
     6121 acacgcttcg acggcgatgc ttcgatcgcg ctcggtaccg gtgcctgcga cgtgcacctc
     6181 gaccggctgc gcgccgagac cgtcgaagtg gtcgcggctg ccatccgcga cgcggtcaca
     6241 acgccgggcg acggctgatc ctcgggcagg tagcatcgcc cgtgtcgtcc gatccggacc
     6301 tcctgcacgc cgatcatccc gccaatgcca ccggcgcgtc gctcgcgcag gtcgcgatcg
     6361 cgtcccacga ctgcaacctg tgcaagctcg cgggtgggcg cacccaggtc gtcttcggaa
     6421 cgggtcgtga ggatgccgat ctgatgttcg tgggtgaggg acccggcgag caggaggaca
     6481 tccagggcat cccgttcgtc gggcgggccg gccagctgtt gacgcagctc atcgaaggca
     6541 tcgggctcac ccgcgacgac gtgtacatcg cgaacgtcgt gaagtgccgg ccgcccggca
     6601 accgcgaccc cgagcccgaa gagatcgaag cgtgcgcacc gtggctcgat cgccagctcg
     6661 cgctcatccg cccgaccgtc atcgtcacgc tcggcaactt cgcgacgaag ctgctcctgg
     6721 gcacgaagga aggcatcacg aaactgcgcg gcaacgagtt cgcgttctcg cgcgcaggga
     6781 tcgatgccgc cttgttcccg acactgcacc cgtccgccgt gctgcgcagt ggtggtgggc
     6841 agaagctcgc ggaagtgcgc tccgacttcg tacaggtcaa gcgcgcgctc gtccggggcg
     6901 catgaagacg accacgacgt cggcggagca cacacgcgca ctcgcggcgg cgatcggcgc
     6961 gcagctcgac gcgggcgacg tcgtcgtgct cgtcggtgag atgggcgcgg gcaagacgac
     7021 gttcgtgcaa ggactcgcac gcggtctcgg cgtcgacgag atcgtcacca gcccgacgtt
     7081 cacgatcgtg caggagtacg acggc
//
LOCUS       DASUGA010000791         2249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_927188, whole genome shotgun sequence.
ACCESSION   DASUGA010000791 DASUGA010000000
VERSION     DASUGA010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927188"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VFX21_15550"
     CDS             <1..242
                     /locus_tag="VFX21_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097443.1"
                     /translation="NRVPLRVPLDLAALEAPGAVPIEQSLLAPAHAGAVAAPERRIPL
                     EPAPTSLEQEITELIERQPEEVAQTLRSWLADRRG"
     gene            247..1251
                     /gene="fliG"
                     /locus_tag="VFX21_15555"
     CDS             247..1251
                     /gene="fliG"
                     /locus_tag="VFX21_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEX5097444.1"
                     /translation="MAAKFTGTQKAAILLMQAGKDRASAVLRSMREQEVAEIMAEVAR
                     LHQLEVNEIEEVLNEFTEIHTARSHIAQGGYGTARELLEASFGGAKADEILDNLGVTM
                     VSAPFEFLRRADTRQVLTYLQDEHPQTIALVLAHMHPDAAAMVLSSLPEELQRDVALR
                     VAKMDRTSPEVIEQVEAILERKLSTVIQQSDFAQAGGLQTLVDILNSSDRTTERLILE
                     GLEEEDGELADEVRNRMFVFEDVKELDDRSIQLVLRSVDAKELAVALKGVDQKVRDKI
                     LKNMSERAAANLSEEIQLLGPIKLKTVEEAQAGIVRVIRTLEEAGQIDMSRGGDELVV
                     "
     gene            1241..1870
                     /locus_tag="VFX21_15560"
     CDS             1241..1870
                     /locus_tag="VFX21_15560"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HEX5097445.1"
                     /translation="MSSDRLARGRVLSAGQVTVRPADLGTVTARPARGIVVSSELVES
                     AKQDGYAVGHDEGYADGYQQGIHAAEGHANLLGQLVQRLAAAADELAAREATARADIE
                     DQVVAAALQIAEVIIGHELAQPDTRGRDAIARALALAPERGHVTARLHPADLAVIDPA
                     ALATGRTLDIIPDPTLAPGDCIVDVGACRVDARIGPALERAREVLEPHE"
     gene            1863..>2249
                     /locus_tag="VFX21_15565"
     CDS             1863..>2249
                     /locus_tag="VFX21_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscN/YscN/HrcN family type III secretion system
                     ATPase"
                     /protein_id="HEX5097446.1"
                     /translation="MSNELLDRVVEGLRPRTMGRVARVVGLNLEVDGLQLPIGAALRV
                     HGDNDLIAEVVAVKDDGLVCIPLGELRGVRAGDFAEAATGATSVPIGNALLGRVLDGL
                     GRPIDNGPLLHHLPHVPVDHGAPHPLT"
BASE COUNT          395 a          793 c          734 g          327 t
ORIGIN      
        1 ccaaccgcgt gccgttgcgg gtgccgctcg acctcgccgc gctcgaagcc ccgggcgcgg
       61 ttccgatcga acagtcgctc ctcgcgccgg cgcacgcggg tgcggtcgcc gcgcccgagc
      121 gacgcatccc gctcgagcct gcgccgacgt cgctcgagca ggagatcacc gaactcatcg
      181 agcgtcagcc cgaagaagtc gcgcagacgc tgcgctcctg gctcgcagat cggcgtggat
      241 agctagatgg cggcgaagtt caccggcacc cagaaggcgg cgatcctgct catgcaagca
      301 ggcaaggacc gcgcgtcggc ggtgctgcgg tcgatgcgcg agcaggaagt ggccgagatc
      361 atggccgagg tcgcgcgcct gcaccagctc gaagtcaacg agatcgaaga ggtcctcaac
      421 gagttcaccg agatccacac cgcccgctcg cacatcgcgc aaggcggtta cggcacggca
      481 cgcgaactgc tcgaggcgag cttcggcggc gccaaggccg atgagattct cgacaacctc
      541 ggcgtgacga tggtttcggc accgttcgag tttctgcgcc gggccgacac ccgccaggtg
      601 ctcacctacc tccaggacga acacccgcag acgatcgcgc tcgtgcttgc acacatgcac
      661 cccgacgcgg cggcgatggt gctcagctcg ctgccagaag aactccagcg cgacgtggcg
      721 ctgcgcgtcg cgaagatgga ccgcacttcg cccgaggtca tcgaacaggt cgaagcgatt
      781 ctcgaacgca agctttcgac ggtcatccag cagtcggact tcgcgcaagc gggcgggctg
      841 cagacactcg tcgacatcct caactcgtcc gaccgcacga cggaacggct catcctcgaa
      901 ggcctcgaag aagaggacgg cgaactcgcc gacgaagttc gcaaccggat gttcgtgttc
      961 gaagacgtca aggaactcga cgaccgctcg atccagctcg tgctgcgctc ggtcgacgcc
     1021 aaggagctgg cggtcgcact caagggtgtc gaccagaagg tgcgcgacaa gattctcaag
     1081 aacatgtcgg aacgcgccgc agccaacctc tccgaagaaa tccagctgct cggcccgatc
     1141 aagctcaaga ccgtggaaga ggcgcaggcg ggcatcgtgc gtgtgatccg cacgctcgaa
     1201 gaagccggcc agatcgacat gtcgcgcggt ggtgatgagc ttgtcgtctg atcgcctcgc
     1261 acgcggccgc gtcctttccg ccggccaggt cacggtgcgt ccggccgacc tcggcacggt
     1321 gaccgcgcgc cccgcgcgcg gcatcgtcgt gagttcggag ctggtcgagt cggccaagca
     1381 ggacggttac gcggttggtc acgacgaggg ctacgccgac ggctaccagc agggcattca
     1441 cgcagccgag gggcatgcga acctgctcgg ccagctcgtg cagcgcctcg cggccgccgc
     1501 cgacgaactc gccgcccgcg aagcaactgc gcgtgccgac atcgaagacc aggtcgtcgc
     1561 ggcggcgttg cagatcgcgg aagtgatcat cggccacgag ctcgcacagc ccgacacgcg
     1621 cggtcgcgat gcgatcgcac gcgccctcgc cctcgcgcct gagcgcggcc acgtcacggc
     1681 gcgcctccac cccgccgacc tcgcggtgat cgacccggcg gcgctcgcga cgggccgcac
     1741 gctcgacatc attcccgatc cgaccctcgc ccccggcgac tgcatcgtcg acgtcggtgc
     1801 gtgccgcgtc gacgcccgca tcggacccgc cctcgaacgc gcgcgggagg tgttggagcc
     1861 gcatgagtaa cgaactcttg gatcgcgtcg tcgaaggact ccggccgcgc acgatgggac
     1921 gcgtcgcgcg tgtcgtcgga ctgaacctcg aagtggacgg cctccagctc ccgatcggtg
     1981 cggcgctacg ggtacacggc gacaacgacc tcatcgccga agtcgtcgcg gtcaaggacg
     2041 acggtctcgt gtgcataccg ctcggtgaac tgcgcggcgt gcgcgccggc gacttcgccg
     2101 aagctgccac cggcgcgacg tcggtaccga tcggcaatgc gctgttgggt cgcgtgctcg
     2161 acgggctcgg ccgccccatc gacaacggac cacttctcca ccatcttccc catgtccctg
     2221 tggatcacgg tgctccgcac ccgttgacg
//
LOCUS       DASUGA010000792         3247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2038428, whole genome shotgun sequence.
ACCESSION   DASUGA010000792 DASUGA010000000
VERSION     DASUGA010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2038428"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VFX21_15570"
     CDS             <1..232
                     /locus_tag="VFX21_15570"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the reversible hydration of unsaturated
                     fatty acyl-CoA to beta-hydroxyacyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX5097447.1"
                     /translation="ELLPFARQLAADIVSNDQMGVRRMLRTYDEAFGTASGAAWDLED
                     KVSREWEGPGFDPASIEARRQKIVDRGRSQIS"
     gene            278..877
                     /locus_tag="VFX21_15575"
     CDS             278..877
                     /locus_tag="VFX21_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5097448.1"
                     /translation="MTISERAAERAVGPARDRASAEVRALVDAAVAVLRRGGAEQLTV
                     AEVLREAGLSTRAFYRHFTSKDELVLAVYERDATASHDRLRRAMADAASARAAFELWV
                     DETLALAFDTRRARRTRVLATEGARLQHQFPEEFDAIVRGMIAPLAELRGISALDARS
                     IHAVTWAVVEAKLRGDAINRDEARAHVLAFCGAAIGFGA"
     gene            874..2193
                     /locus_tag="VFX21_15580"
     CDS             874..2193
                     /locus_tag="VFX21_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HEX5097449.1"
                     /translation="MKLPPVDEKYFDREYETMARPQIDALQEELLLEMVPYAYEHSPL
                     TRATWDAAGVHPRDIRSLDDFRERAPFVDKDTLRHFRDERRDPFGGLCTLATSELTAV
                     MSTSGTTGDPTLVPEKWGGGGGGRPTIITRDFWGMGVRPGDYLALVLFTFRGPTYGLF
                     QGLGTIPLLFDFDPAEMARFCELSLRYRPTGLYNFGTVLINAVRDACEQHGLDPVDVF
                     SSYKGVVFAGEPLSPRARALAESWDVELYEHCGVGDVTAAFECEHHDGLHFWEDSAFV
                     EGLDPDGDAPVADGERCELVATSLVNRTAPLVRYRSDDIVRITRETCRCGRTHARMWP
                     IGRKGDEVVVDGRAILPIDIWHAVETVDACAMGLFQVIRTTRDTDELRLRVGFAPAWE
                     DRQGTVRDEVQAAVRAATGIVPVVELVPNAALLRLGPPHKIPRVAAR"
     gene            2190..2843
                     /locus_tag="VFX21_15585"
     CDS             2190..2843
                     /locus_tag="VFX21_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097450.1"
                     /translation="MTALWGVGACDDGVTRVPWEISHDEIQREMGAAPAALAALGVGP
                     GERVLFCSMLSEAGQFWPLIVGAMLAGAQLSCADANEGEAVRVAMFTRLMAYRAVLGV
                     TPALLDGLDALDQSYADTFASVAVVGARPGAYERLERSGCTPHRFVLCGPAVAIGREP
                     GGPAYVNDDEWELGLDGDHIVVTAKRPRATTFDRAPTAIRGALVDGGVVPSPVKEER"
     gene            2840..>3247
                     /locus_tag="VFX21_15590"
     CDS             2840..>3247
                     /locus_tag="VFX21_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5097451.1"
                     /translation="MTKRALISADNHVFEPVTLWQERLPHQYRDRGPRLEEDGEWLVM
                     AIEGMPNRKLTRVDGGAANGEAKKSHEMGMRAGGADPDARLQDMEHDGVVAEVIYPTF
                     GLFIDMIPATDLQMACAQVYNDWLAETFLHRPDV"
BASE COUNT          466 a         1108 c         1181 g          492 t
ORIGIN      
        1 cgagctgctc ccgttcgccc ggcagctcgc ggccgacatc gtctccaacg accagatggg
       61 cgtgcgccgc atgctccgca cctacgacga ggcgttcggc acggcctccg gcgcggcctg
      121 ggatctcgag gacaaggtca gccgcgagtg ggaggggccg gggttcgatc cggcgtcgat
      181 cgaggcccgt cgccagaaga tcgtcgaccg ggggcgatcc cagatctcct gatcgcttga
      241 caaacggtcg ctgaaaacat cattctcgcg cctgagcatg acgatttcgg agcgcgctgc
      301 cgaacgggcc gtgggcccgg cccgcgaccg ggcgtccgcc gaggtgcggg cgctcgtcga
      361 cgcggccgtc gcggtgctgc gccggggcgg cgccgagcag ctcaccgtcg cggaggtgtt
      421 gcgcgaagcc gggctctcga cgcgcgcctt ctaccggcac ttcacctcga aggatgagct
      481 ggtgctggcc gtgtacgagc gggacgcgac ggcgtcgcac gaccggttgc gacgcgccat
      541 ggccgatgcg gcctccgcac gcgcggcctt cgagctctgg gtcgacgaga cgttggcgct
      601 cgcgttcgac acgcgccgcg cccgccggac gcgcgtgctc gcgaccgaag gcgcgcgttt
      661 gcagcatcag ttccccgagg agttcgacgc gatcgtgcgc ggcatgatcg caccgctcgc
      721 cgagctccgg ggcatctcgg ccctcgacgc ccgctcgatc cacgcggtga cgtgggccgt
      781 cgtcgaggca aagctgcgcg gcgacgcgat caatcgcgac gaagctcgcg cgcacgtgct
      841 cgcgttctgc ggcgcggcga tcggattcgg cgcgtgaaac tcccgcccgt cgacgagaag
      901 tacttcgacc gcgagtacga gacgatggcg cgcccgcaga tcgacgcgct ccaggaagag
      961 ctgctgctcg agatggtgcc gtacgcgtac gagcactcgc cgctgacgcg cgccacgtgg
     1021 gatgccgccg gcgtgcaccc gcgcgacatc cgttcgctcg acgacttccg tgagcgggcg
     1081 ccgttcgtcg acaaggacac gctccgccat ttccgtgacg agcggcgcga cccgttcggc
     1141 gggctgtgca ccctcgcgac gagcgagctg accgcggtga tgtcgacgtc gggcacgacg
     1201 ggtgatccga ccctcgtccc cgagaagtgg ggcggtggcg gcggcggccg gccgacgatc
     1261 atcacgcgcg acttctgggg gatgggcgtg cggcccggcg actacctggc cctcgtgttg
     1321 ttcacgttcc gcgggccgac gtacgggttg ttccagggcc tcggcacgat cccgctcctg
     1381 ttcgatttcg acccggccga gatggcgcgc ttctgtgagc tgtcgctgcg ctaccggccg
     1441 accgggctgt acaacttcgg caccgtgctc attaacgcgg tgcgcgacgc gtgcgagcag
     1501 cacggtctcg atccggtcga cgtcttctcg tcgtacaagg gtgtcgtctt cgcgggcgag
     1561 ccactgagcc cgcgcgcccg cgcgctcgcc gagagctggg acgtcgagct ctacgagcac
     1621 tgcggtgtcg gtgacgtcac cgcggcgttc gagtgcgagc accacgacgg gctgcatttc
     1681 tgggaggact ccgcgttcgt cgagggcctc gatcccgacg gtgatgcgcc ggttgccgac
     1741 ggcgaacgct gcgagctggt cgccacgtcg ctggtgaacc ggaccgcgcc actcgtgcgc
     1801 taccgctcgg acgacatcgt ccgcatcacg cgcgagacgt gccgctgcgg ccgcacgcac
     1861 gcgcgcatgt ggccgatcgg ccgcaagggc gacgaagtcg tcgtcgacgg gcgggccatt
     1921 ctcccgatcg acatctggca cgcggtggaa accgtcgatg catgcgcgat gggactcttc
     1981 caagtgatcc gcaccacgcg cgacaccgac gagctgcgac tgcgcgtcgg tttcgcaccg
     2041 gcgtgggagg accgccaagg aacggtgcgc gacgaggtgc aggcggcggt gcgtgccgcg
     2101 accggcatcg tcccggtcgt cgaactcgtt ccgaacgcgg ccctgctgcg tctcggtccg
     2161 ccgcacaaga tcccgcgcgt ggcagcccga tgaccgcgct gtggggcgtc ggtgcctgtg
     2221 acgacggcgt gacgcgggtg ccgtgggaga tctcgcacga cgagatccag cgggagatgg
     2281 gcgcggcgcc ggcggcgttg gccgcgctcg gggtcgggcc cggggagcga gtgctgttct
     2341 gctcgatgct gtccgaggcg ggtcagttct ggccgctgat cgtcggcgcc atgctggcgg
     2401 gcgcgcaact gtcgtgtgcc gacgccaacg aaggtgaagc ggtgcgtgtc gcgatgttca
     2461 cgcgcctcat ggcgtaccgc gcggtgctcg gcgtgacgcc cgcgttgctc gacgggctcg
     2521 atgcgctcga ccagtcgtac gcggacacgt tcgccagtgt tgccgtcgtc ggtgcccggc
     2581 ccggcgcata cgaacggctc gagcgctcgg ggtgcacgcc gcaccgattc gtgctgtgcg
     2641 ggcccgcggt cgcgatcggg cgcgagcccg gcggccccgc gtacgtgaac gacgacgagt
     2701 gggagctcgg cctcgacggc gatcacatcg ttgtcacggc caagcgcccg cgcgcaacga
     2761 cattcgaccg cgcgccgacc gcgatacgcg gcgcgctcgt ggacggcggc gtcgtgccgt
     2821 cacccgtgaa ggaggaacga tgaccaagcg cgcgctgatc agcgccgaca accacgtgtt
     2881 cgagccggtc acgctgtggc aggaacgcct gccgcatcag taccgcgacc gcggcccgcg
     2941 cctcgaggaa gacggcgagt ggctcgtcat ggcgatcgag ggcatgccca accggaagct
     3001 gacgcgcgtc gacggcggcg ccgcgaacgg ggaggcgaag aagtcgcacg agatgggcat
     3061 gcgcgccggt ggcgcggatc ccgacgcgcg gttgcaggac atggagcacg acggcgtcgt
     3121 cgccgaagtg atctatccga cgttcggctt gttcatcgac atgatccccg cgaccgacct
     3181 gcagatggcc tgcgcgcagg tctacaacga ctggctcgcg gagacgtttc tgcaccggcc
     3241 cgacgtg
//
LOCUS       DASUGA010000793         2942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_233346, whole genome shotgun sequence.
ACCESSION   DASUGA010000793 DASUGA010000000
VERSION     DASUGA010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233346"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..752
                     /locus_tag="VFX21_15595"
     CDS             51..752
                     /locus_tag="VFX21_15595"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX5097452.1"
                     /translation="MPRNLRQAFELADSYGLLLALLLTDYVLLMLSSPDAPLRILQSL
                     FVGATVILAFHTSRARPRLVHFAVVCAVLVVVVSIGRLIFDTNELGGLGAVMLGILIL
                     LSPPVILRRVLGHERVGLETILGALCVYLMIGLLFAYTYASIHGFTDEVFVQQEEIVP
                     SDYVYFSVVTLTTTGYGDLTPAAGAPRAAAMIEMLTGQIFLVTLVARLVALFGEERRT
                     VEPAAPARGDPERLE"
     gene            complement(756..1679)
                     /locus_tag="VFX21_15600"
     CDS             complement(756..1679)
                     /locus_tag="VFX21_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5097453.1"
                     /translation="MARPIPARAWILLPLLLALAAPATPAAAQTRYPVPYLFSANVIA
                     AATQPGSAPPGANDWSCRPSRSHPRPVVLVHGLLATMTDNWQTMSPLLANNGYCVFAL
                     TYGTQDSEMFGGLTRMEHSAAELRAFVNQVLTTTGASKVDIVGHSEGATMPDYYIEYL
                     GGAKRVAHYVGVSGVKHGTTLHGVATTAALIQSFFPGAPSPTSGLCDSCDQFIVGSEY
                     IQKIEARAPAAGVIYTNIATRYDELVSPYTSSFLAGPNVSNITVQDRCALDASDHLSI
                     ISSPRTGRMILNALDPARAQPVPCTVVLPAA"
     gene            1780..2772
                     /locus_tag="VFX21_15605"
     CDS             1780..2772
                     /locus_tag="VFX21_15605"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX5097454.1"
                     /translation="MTDRAKSSLNRLIGSIVGPVMDAVEPDDLLERVDVNAILERIDI
                     DELLQHIDVNALMARIDIDQLLDRIDIDAMLDRIDVNRMMARVDIDGMLDRIDVNGMV
                     ERVDINAIVEKAHIEEMISRSTRGITQNLLDLVRRQLVGLDVILDRIISRFRRKRPDG
                     SPIETTAAGATRLTAFFLDSIILTTMFSIGVSISAYIVNLFLQSDWKPEGGSSLGWLL
                     AFLGWSFVYFVSGWAVAGRTLGMGLVGLRVVTKDAHAISFGAALVRMIFFPTMFVFGI
                     GAIGIVLGRRHRGFPDLTARTIVVYDWGERTVEAPRPLSHWFVQHGVDPALPHP"
BASE COUNT          461 a          968 c          981 g          532 t
ORIGIN      
        1 gattcgggtg agccggggcc gaggagtgtc ccgcaattac ggttgcgccc gtgccgcgga
       61 atctgaggca ggcgttcgag ctcgcggaca gctacggctt gctcctggcg ctgctgctca
      121 ccgactacgt gctgctgatg ctctcgagcc cggacgcgcc gctgcggatc ctccagtcgc
      181 tgttcgtcgg ggcgacggtg atcctcgcgt tccacacgtc gcgagcacga ccgcggctcg
      241 tgcacttcgc cgtcgtctgc gcggtcctcg tcgtcgtcgt gtcgatcggg cgtctcatct
      301 tcgacaccaa cgaactgggt gggttgggcg cggtgatgct cggcatcctc atcttgctca
      361 gcccgccggt gatcttgcgg cgcgtgctcg gtcacgaacg ggtcgggctg gagacgatcc
      421 tcggtgcgtt gtgtgtgtac ctgatgatcg gtctcttgtt cgcgtacaca tacgcgtcga
      481 tccacggatt cacggacgag gtgttcgtgc agcaggaaga gatcgtcccg tccgactacg
      541 tgtacttcag cgtggtcacg ttgacgacga ccggctacgg cgacctcacg ccggccgccg
      601 gcgcgccgcg cgcggccgcg atgatcgaga tgctcacagg tcagatcttt ctcgtcacgc
      661 tcgtcgcgcg cctcgtcgcg ctgttcggcg aggagcgccg aaccgtcgag ccggctgcac
      721 cggctcgcgg cgaccccgaa cggctcgagt gactgctatg ccgccggcaa cacgaccgtg
      781 cacgggaccg gttgcgcacg ggccgggtcg agtgcattca agatcattcg cccggtgcgc
      841 gggctcgaga tgatcgaaag gtgatcgctc gcatcgaggg cacaccgatc ctgcacagtg
      901 atgttgctga cgttcgggcc ggccaggaag ctgctcgtat acgggctgac gagctcgtcg
      961 tagcgcgtgg cgatgttcgt gtagatcaca cccgcggccg gcgcgcgcgc ttcgatcttc
     1021 tggatgtact cggacccgac gatgaactgg tcacacgagt cgcacaggcc cgaggtcggg
     1081 ctgggcgcgc ccgggaagaa tgattggatc aacgcggcgg tggtcgcgac gccgtgcaac
     1141 gtcgttccgt gcttgacacc cgacacaccg acgtagtgcg cgactctctt cgcgccaccg
     1201 agatattcga tgtagtagtc gggcatcgtc gcgccctcgg aatggccgac gatgtcgacc
     1261 ttgctcgcgc ccgtcgtcgt gagcacctga ttcacgaacg cgcgcagctc ggccgcgctg
     1321 tgctccatgc gggtcagacc gccgaacatc tccgagtcct gagttccata tgtgagcgcg
     1381 aacacgcagt agccgttgtt ggcgagcagc ggcgacatcg tctgccagtt gtcggtcatc
     1441 gtcgcgagca gtccgtgtac gaggacgacc ggccgcgggt gcgagcggct cgggcggcac
     1501 gaccagtcgt tggcaccggg cggcgccgaa ccgggctgcg tcgcggcggc gatcacgttg
     1561 gccgagaaca gatacggcac cgggtaccgg gtctgcgcgg cggccggggt ggcgggcgcg
     1621 gcgagggcga gcaggagggg cagcaggatc catgcacgcg cgggaatggg gcgggccatg
     1681 ggccgttccg taccgaacga gtcccgaaat catgccggcg ccgtgtcacg atgtgccgat
     1741 gacggctttc acgcctgcgg agatccgggt gagcgaccga tgacggaccg ggccaagtcg
     1801 tcgctgaacc ggctgatcgg gtcgatcgtc ggcccggtca tggacgcggt cgaaccggac
     1861 gatctcctcg agcgagtcga cgtcaacgcc atcctcgagc ggatcgacat cgacgaattg
     1921 ctccagcaca tcgacgtcaa cgcgctcatg gcgcgcatcg acatcgacca gctgctcgac
     1981 cggatcgaca tcgacgcgat gctcgaccgc atcgacgtga accggatgat ggctcgggtc
     2041 gacatcgacg gcatgctcga ccgtatcgac gtgaacggca tggtcgagcg ggtcgacatc
     2101 aacgccatcg tcgagaaggc tcacatcgag gagatgatct cgcgcagcac gcgcggcatc
     2161 acgcagaacc tgctcgacct cgtgcgccgg caactcgtcg gcctcgacgt gatcctcgac
     2221 cgcatcatct cgcgctttcg ccgcaagcgg ccggacggtt cgccgatcga aactacggcg
     2281 gccggcgcga cgcggctcac cgcgttcttc ctcgactcga tcatcctcac gacgatgttc
     2341 agcatcgggg tgtccatctc ggcctacatc gtcaacttgt tcctccagag cgactggaag
     2401 cccgaaggcg ggagcagcct cggttggctg ctcgccttcc tcggctggag tttcgtctac
     2461 ttcgtgtcag ggtgggccgt cgccggccgc acgctcggca tggggctcgt gggcctgcgc
     2521 gtcgtgacca aggacgcgca cgcgatctcg ttcggtgccg cgctcgtacg catgatcttc
     2581 ttcccgacga tgttcgtctt cggcatcggt gcgatcggca tcgtcctcgg gcgccgccac
     2641 cgcggtttcc cggacctcac ggcccgcacg attgtcgtgt acgactgggg cgagcgcacc
     2701 gtcgaagcac cacgcccgtt gtcgcactgg ttcgtgcaac acggtgtcga tcctgcgctg
     2761 ccgcacccgt aatctcggtg tgtgaagctc acgcccgaca tgcagcgggt cgtgctcgag
     2821 cagcgactgg gcttcgtcgc gaccgtcggc gccgacggcc ggcccaatct ctcgcccaag
     2881 ggcaccacga ccgtgttcga cgacgaacac ctgatgttcg ccgacgtctt ctcgccccgg
     2941 ac
//
LOCUS       DASUGA010000794         3610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1481960, whole genome shotgun sequence.
ACCESSION   DASUGA010000794 DASUGA010000000
VERSION     DASUGA010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481960"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..604
                     /locus_tag="VFX21_15610"
     CDS             116..604
                     /locus_tag="VFX21_15610"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017578313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEX5097455.1"
                     /translation="MSLYDTKIAALDGSPDVLASQKGNVTLMVNVASKCGLTPQYTGL
                     EELHEKYAGQGFSVIGIPCNQFGEQEPGSPDEIATFCSTTYGVTFPLSEKIEVNGDGR
                     HQLYQELTKTKDAEGHDGDIRWNFEKFLVGRDGTVVARFGPLVEPANEDLVSAIEKEV
                     AS"
     gene            complement(573..1205)
                     /locus_tag="VFX21_15615"
     CDS             complement(573..1205)
                     /locus_tag="VFX21_15615"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014284238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX5097456.1"
                     /translation="MADVVRGPDGVTRCGWGGSTPDYCAYHDDEWGRPVVDDVRLYEK
                     LCLEGFQSGLSWLTILRKRENFRNAFAGFEPARVAAFTSKDVTRLLHDAGIVRHRGKV
                     EAAIANARATIAVQDEVGSLSALIWEFEPKGRRAAPKSYADMTSKTDESKALSKELLR
                     RGFRFVGPTTVYSMMQSCGLVNDHLARCCARAMCEAERRTLTRPLPSRSR"
     gene            1229..2023
                     /locus_tag="VFX21_15620"
     CDS             1229..2023
                     /locus_tag="VFX21_15620"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX5097457.1"
                     /translation="MTTLLDRFRLDGQVAIVTGAGKGIGAGIASGLADAGADVVCAAR
                     TVADLEATAAAVRERGRRSLVVPTDVTETAQLEVLVGATMGEFGRLDILVNNAGGWLP
                     RPVLETSERSFEAALRFNVTAPFLLSKLATPHLAQGEGGSIVNISSRAASMVQPVFVA
                     YATAKAALSMMTRAMAPELAPKVRVNAIEVGGVETEALAHVLLDDDVRRRLVANTPMH
                     RVGQPDDIAASVVYLASPAASWVTGKIFEVDGGVESPAFTIPFTEL"
     gene            complement(2010..3062)
                     /locus_tag="VFX21_15625"
     CDS             complement(2010..3062)
                     /locus_tag="VFX21_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HEX5097458.1"
                     /translation="MYRVIQWATGFVGQEAIKGVLAHPELELVGCWVHSPDKVGKDVG
                     EICGIDPVGVKAVGTLDETCAIDADAVVYAPVMASTRDVIRLLESGKNIVTPVGWIYP
                     GDTPGVAAVQAACLAGNVTLHGTGINPGGITERFPLMLSSLCRDITHVRAEEFSDIRN
                     YPTEIVVREIMLFGKSPEEAAKSPMADILGHGFKQSIDMIATGLGWTLDAEKRTTHEM
                     AVATKDLDTPVGLIPKGTCAAQRFTWEGTVRGRTVITVRVNWLMGETDLDPAWSFGPE
                     GQRFEVAFDATPPVSAVFHGLHPPVVGEHPEIGLIPPAMHCVNAIPYVVDADPGIKTY
                     LDLPLLAGRAAPITAR"
     gene            3101..>3610
                     /locus_tag="VFX21_15630"
     CDS             3101..>3610
                     /locus_tag="VFX21_15630"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5097459.1"
                     /translation="MSRDWRAWHTAYDADTPLAQRLVIVQRAIADVLDAAPPGLIRAV
                     SMCTGEGRDLLGVLPEHPRAGDVRARLVELDPELATRARASAPATVEVVCADAAETDV
                     YAGAVPANVVLVCGVFGNIPDDDIQRTIRTLPSLCAAEAFVIWTRHRRPPDRTIDIRR
                     WFAEAGFETH"
BASE COUNT          592 a         1266 c         1185 g          567 t
ORIGIN      
        1 cgaacacgtc ggcgtgggcg gtaccgcgcg ggcgactgtc gccgcggtgg acctcgactg
       61 cgtcggtctg ctggaatccc gactcggatg agctgaatcg caaaggggag ccccaatgtc
      121 gctgtacgac accaagatcg ccgcactcga cggatcgccc gacgttctcg cgtcgcagaa
      181 gggcaacgtc accttgatgg tgaacgtcgc gtcgaagtgc ggtctgacgc cgcagtacac
      241 cgggctcgag gagttgcacg agaagtacgc gggccagggc ttctccgtca tcggcatccc
      301 gtgcaaccag ttcggcgagc aggagccggg atctcccgac gagatcgcga ccttctgcag
      361 cacgacctac ggcgtcacgt tcccgctgtc cgagaagatc gaagtgaacg gtgacggccg
      421 ccaccagctc taccaagagc tgacgaagac caaggacgcc gaaggccacg acggcgacat
      481 ccgctggaac ttcgagaagt tcctcgtcgg tcgcgacggc acggtcgtcg cccgcttcgg
      541 gccgctcgtc gagcccgcca acgaggacct cgtcagcgcg atcgagaagg aagtggcctc
      601 gtaagagtcc gccgttcggc ttcgcacatc gcccgcgcac agcagcgcgc gaggtggtcg
      661 ttcaccagtc cgcacgactg catcatcgag tagacggtcg tcgggccgac gaagcgaaag
      721 ccccggcgaa gaagctcctt cgacagcgct ttcgactcgt cggtcttcga cgtcatgtcg
      781 gcatacgact tgggcgccgc gcgccggccc ttcggttcga actcccagat caacgcggag
      841 agcgaaccga cctcgtcctg cacggcgatc gtggcgcgcg cgttcgcaat cgcggcctcg
      901 accttgccgc ggtggcggac gatgcccgca tcgtgcaaca agcgcgtcac gtccttgctc
      961 gtgaacgcgg cgacgcgtgc cggttcgaag ccggcgaatg cgttgcggaa gttctcgcgc
     1021 ttgcgcaaga tcgtgagcca cgagagcccc gactggaacc cttcgaggca gagcttctcg
     1081 tagagccgca cgtcgtcgac gacgggtcgt ccccactcgt cgtcgtggta cgcgcagtag
     1141 tcgggtgtgc tcccgcccca cccgcaccgc gtcactccat cgggacctcg cacgacgtcc
     1201 gccacgcctt gaaagtagcg tccgtctcat gaccactttg ctcgatcggt tccgcctcga
     1261 cggccaggtt gcgatcgtca ccggtgcggg caagggcatc ggcgcgggca ttgcaagcgg
     1321 gctggccgac gcaggcgcag acgtcgtgtg cgccgcccgc acggtcgcag atctcgaagc
     1381 aactgcggcc gcggtgcgcg aacgcggtcg ccgctccctc gtcgtcccga ccgacgtcac
     1441 cgagacggca cagctcgaag tcctcgtcgg cgcgaccatg ggcgagttcg ggcggctcga
     1501 catcctcgtg aacaacgccg gcggctggct gccacgtccg gtgctcgaga cgagtgaacg
     1561 gtccttcgag gccgcgctgc gcttcaacgt caccgcgccg ttcctgctgt ccaagctcgc
     1621 gaccccgcat ctcgcgcagg gcgagggtgg aagcatcgtc aacatctcgt cgcgcgccgc
     1681 gagcatggtg cagccggtct tcgtcgccta cgcaacggcc aaggccgcgc tgtcgatgat
     1741 gacgcgcgcg atggcgcccg agctcgcgcc gaaggtccgc gtgaacgcga tcgaggtcgg
     1801 cggcgtcgag accgaggccc tcgcgcacgt actgctcgac gacgacgtgc gccgccgact
     1861 cgttgcgaac acgccgatgc atcgtgtcgg ccagcccgac gacatcgccg cgtcggtcgt
     1921 ctacctcgcg tcgcccgccg cgagctgggt gacgggcaag atcttcgaag tcgacggcgg
     1981 cgtcgagtcg cccgcgttca cgatcccctt caccgagctg taatcggcgc cgcgcgccca
     2041 gcgaggagcg ggaggtcgag gtacgtcttg atcccggggt cggcgtcgac gacgtacggg
     2101 atcgcgttga cgcagtgcat cgcaggcggg atgagcccga tctcgggatg ctcgccgaca
     2161 accggcgggt gcaagccgtg gaagaccgcg gagaccggcg gcgtcgcgtc gaacgcgacc
     2221 tcgaaccgct gaccttcggg gccgaacgac cacgccggat cgagatcggt ctcacccatc
     2281 aaccagttca cgcgcaccgt gatcacggtc cggccgcgca cggtgccttc ccaggtgaag
     2341 cgctgcgcgg cgcacgtgcc cttggggatg agcccgacgg gcgtgtcgag atccttcgtc
     2401 gcgacggcca tctcgtgcgt cgtgcgcttc tcggcgtcga gcgtccaacc gagccccgtc
     2461 gcgatcatgt cgatcgactg cttgaacccg tgaccgagga tgtcggccat cgggctcttg
     2521 gccgcttcct cgggcgactt gccgaacaac atgatctcgc gcacgacgat ctcggtcggg
     2581 tagttgcgga tgtcggagaa ctcttcggcg cgtacgtgcg tgatgtcgcg acacagcgac
     2641 gacaacatga gcgggaaacg ttcggtgatc ccgcccgggt tgatgccggt gccgtgcagc
     2701 gtcacgttgc ccgcgaggca cgcggcctgc accgcggcga caccgggtgt gtcaccggga
     2761 tagatccagc cgacgggtgt cacgatgttc ttgcccgact ccagcaggcg gatcacatcg
     2821 cgcgtgctcg ccatcaccgg tgcgtacacg accgcgtcgg cgtcgatcgc gcaggtctcg
     2881 tcgagcgtgc cgacggcctt cacaccgact ggatcgatgc cgcagatctc gccgacgtcc
     2941 ttgccgacct tgtccggact gtgcacccaa caacccacga gctccagctc ggggtgcgcg
     3001 agcacgccct tgatcgcctc ctgaccgacg aatccggtcg cccattggat aactcggtac
     3061 atggcccgga gactacgacc gccggcaata gcctccggcc atgagccgcg actggcgggc
     3121 ctggcacacg gcctacgacg ccgatacacc gctcgcccag cggctcgtca tcgtgcagcg
     3181 cgcgatcgcc gacgtgctcg acgcggcgcc gcccggactg atccgtgccg tcagcatgtg
     3241 cacgggcgaa ggccgcgatc tgctcggtgt gctgcccgag caccctcgcg cgggcgacgt
     3301 acgcgctcgc ctcgtcgagc tcgatcccga actcgcgacg cgcgcccgcg cgtcggcccc
     3361 cgcgacggtc gaggtggtat gcgccgacgc ggccgaaacc gacgtgtacg cgggcgccgt
     3421 ccccgcgaac gtcgtgctcg tctgcggcgt gttcggcaac attcccgacg acgacattca
     3481 gcgaacgatc cgcacactcc cgtcgctgtg cgcagccgaa gcgttcgtca tctggacgcg
     3541 ccaccgccgc ccacccgatc ggacgatcga catccggcgc tggttcgcgg aagccgggtt
     3601 cgaaacgcac
//
LOCUS       DASUGA010000795         2163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_371514, whole genome shotgun sequence.
ACCESSION   DASUGA010000795 DASUGA010000000
VERSION     DASUGA010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371514"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFX21_15635"
     CDS             <1..324
                     /locus_tag="VFX21_15635"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX5097460.1"
                     /translation="DMIISGGVNIYPAEIEAVLYAYPKVLDAAVFGVPDDEWGERVHA
                     IVQAKAGETIDLADLQTFAESRLARYKQPRAYEVRDALPRTDSGKLLKRVLRDEYWKG
                     QERAV"
     gene            321..740
                     /locus_tag="VFX21_15640"
     CDS             321..740
                     /locus_tag="VFX21_15640"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5097461.1"
                     /translation="MSERAVFDHVNIVGRDMQATIAFYERLGVEVNRPPAPWDRHHVN
                     TETDVDFDSTEFATHWNESWRAGETGVVINFRLASREAVDETYGNLTGAGYEGKQAPW
                     DAFWGARFAIVADPDGNLVGFMSPSDPARRYDVQPPA"
     gene            828..>2163
                     /locus_tag="VFX21_15645"
     CDS             828..>2163
                     /locus_tag="VFX21_15645"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX5097462.1"
                     /translation="MPTLSEAASKELLATYGVPVLDERTVDTADAAVAAATDLGYPVV
                     LKLCGDAIAHKTERGLVRLSLRDEGAVRDAATELLAAATPEDGDVGLLIAPMVSGNRE
                     LIAGLVRDPQFGPCVMIGVGGVLAEALGDVAFRLVPLEGIDTFEIVDDLATRKLFGEF
                     RGEPPVDRHSLARVLTALSQLATERPDIVSVDLNPLIIVEGRPVAVDALVELGDDVPA
                     PGPAPTRVADQRFRALFEPRGVVVAGASTHPGKFGFVALHNILAAGYEGKVGATNLDG
                     TAVLGVDTVASVDDLPDGPWDLVFVCTPAAANPDLLRACAKKGIRAAFLTSAGYGEAG
                     PDGQQAERELVALADDLGILLAGPNGQGVVSPPAKLFAQIVAPYPPAGRIAIASQSGN
                     FVSSFENWAVQTGVGVSRAVSAGNAAAIGIADYLEWYAEDDETAVSLAYVEGV"
BASE COUNT          338 a          770 c          746 g          309 t
ORIGIN      
        1 gacatgatca tctcgggcgg cgtcaacatc tacccggccg agatcgaggc cgtgttgtac
       61 gcgtacccga aggtgctcga cgcagccgtg ttcggcgtcc ccgacgacga atggggcgag
      121 cgcgtccatg cgatcgtgca ggcgaaggcg ggcgagacga tcgacctcgc ggacttgcag
      181 acattcgccg agtcgcgtct cgcgcgctac aagcaaccgc gcgcgtacga agtgcgcgac
      241 gcgctgcccc gcaccgattc cggcaagctg ctcaagcgcg tgctgcgcga cgagtactgg
      301 aaaggccagg agcgcgcggt atgagcgaac gtgcggtctt cgaccacgtc aacatcgtcg
      361 ggcgcgacat gcaggcgacg atcgcgttct acgagcggct cggcgtcgag gtcaatcggc
      421 cgcccgcacc gtgggaccgg catcacgtca acaccgagac cgacgtcgac ttcgacagca
      481 ccgagttcgc gacgcactgg aacgagtcgt ggcgcgcggg ggagaccggc gtcgtcatca
      541 acttccggct cgcgtcgcgc gaagcggtcg acgagaccta cggcaacctc accggcgcgg
      601 gttacgaagg gaagcaggcc ccgtgggacg cgttctgggg tgcgcgcttc gcgatcgtcg
      661 ccgaccccga cggcaacctc gtcggcttca tgagcccgag cgaccccgcc cgccggtacg
      721 acgtgcaacc accggcgtaa acggagagcg tggtcacgaa attcgcaaca tgtcgcggat
      781 ttcgtgacca cggcccgcag aacggccgcc gctacgatcc gggccctgtg ccgacgctct
      841 cggaagctgc atcgaaggag ttgctggcca cgtacggcgt gccggtgctc gacgagcgca
      901 ccgtcgacac cgcggacgcg gcagtcgccg ccgccaccga cctcggctat ccggtggtgc
      961 tcaagctgtg cggcgacgcg atcgcgcaca agaccgagcg gggcctcgtc cggctgagcc
     1021 tgcgcgacga aggcgcggtc cgcgacgcgg ccactgaact gctcgcggcc gcgacacccg
     1081 aagacggcga cgtcggcctg ctcatcgcgc cgatggtgtc gggcaatcgc gaactgatcg
     1141 ccggcctcgt gcgcgacccg cagttcggcc cgtgcgtgat gatcggcgtc ggtggcgtgt
     1201 tggcggaagc gctcggcgac gtcgcgttcc ggctcgtacc cctcgaaggc atcgacacgt
     1261 tcgagatcgt cgacgacctc gcgacgcgca agctgttcgg cgagttccgc ggcgagccgc
     1321 ccgtcgaccg gcactcgctc gcacgcgtcc tcaccgcgct gtcgcaactc gccaccgaac
     1381 ggcccgacat tgtgtcggtc gacctcaacc cattgatcat cgtcgagggt cgcccggtcg
     1441 cggtcgacgc gctcgtcgag ctgggcgacg atgtgcccgc gccgggaccg gccccgaccc
     1501 gcgtcgccga ccaacggttc cgcgcgttgt tcgagccgcg cggtgtcgtg gtcgccggcg
     1561 cgtcgacgca cccgggcaag ttcgggttcg tcgcgctgca caacatcctg gcggccggct
     1621 acgagggcaa ggtcggggcc accaacctcg acggcaccgc ggtgctcggc gtcgacaccg
     1681 tcgcgagcgt cgacgacctg cccgacggcc cgtgggacct cgtgttcgtg tgcacgcccg
     1741 cggccgccaa ccccgatctg cttcgcgcgt gcgccaagaa gggcatccgc gccgcgttcc
     1801 tcaccagcgc gggctacggc gaagcaggtc ccgacgggca acaggccgaa cgcgagctcg
     1861 tcgcgctcgc cgacgacctc ggcatcctcc tggccgggcc gaacggccag ggtgtcgtgt
     1921 cgccgcccgc caagctgttc gcgcagatcg ttgcgccgta cccgccggcc ggtcgtatcg
     1981 cgatcgcgag ccagtcgggc aacttcgtgt cgtcgttcga gaactgggcg gtgcagaccg
     2041 gtgtcggcgt gagccgcgcc gtcagcgcgg gcaacgcggc cgcgatcggc atcgccgact
     2101 acctcgagtg gtacgccgag gacgacgaga ccgcggtcag cctcgcctac gtcgagggcg
     2161 tcc
//
LOCUS       DASUGA010000796         2327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1254875, whole genome shotgun sequence.
ACCESSION   DASUGA010000796 DASUGA010000000
VERSION     DASUGA010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254875"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="VFX21_15650"
     CDS             <1..1150
                     /locus_tag="VFX21_15650"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5097463.1"
                     /translation="SPERDFGPRSDDDVYILYTGGTTGMPKGVVWRQEDVLCVLGGLV
                     AFDTGIRVSDEWEWAKAAQAQDVPTKSVVIAPLMHGAAQWTVLNGLLKGTTTVMLPKF
                     EPDEVWKAVDAHQIPTIAITGDAMGRPLIEAFEAGEYDGSSLVTVASTAAIFSATVKD
                     RYLEQFPNLIIADAVGSTETGYNGVRVIVKGVEQPLGPPTINMGPDTVVLDDDLNIVE
                     PGSDVVGRMARGGNVPLRYHKDPEKSAATFVTAKNGKRYSIPGDFARVEADGSMTLLG
                     RGSVCINSGGEKIFPEEVESALKAHVAVFDAVVVGVPDERWGERVAAVVEVRVDATIT
                     LEELDAHSRELIAGYKVPRELHVVPKIERSPSGKPDYRWAKSVALGEI"
     gene            complement(1151..1468)
                     /locus_tag="VFX21_15655"
     CDS             complement(1151..1468)
                     /locus_tag="VFX21_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097464.1"
                     /translation="MSEDRVLGGVGSKVVYEDDRVRVWVLKLAPGERSAIHQHELDHL
                     LIQVQGDRIAVDPEPDTASPYKDYFAADVIPGMVTFVPAGGIETAVNCGEQQYREIIV
                     ELK"
     gene            1503..2324
                     /locus_tag="VFX21_15660"
     CDS             1503..2324
                     /locus_tag="VFX21_15660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX5097465.1"
                     /translation="MPRSLRNGSVSFGLVNVPIALYTAIEPKDIHFHQMTKSGHRIRQ
                     KRVDEKTGREVEYKSIVKGYEQSKGKYVMVEPEEIDAAAPKQTRAIEIEEFVDLAEID
                     PIYYNSTYYVAPGKGAGADKGYALLRDVMEKSGRAAIGRFVMRTKQYLAAIRVDKGTL
                     VLHTLYFADEIRDTKGLGIPSRTKAGDREVRIARQLVDSLTVEWDPKRHKDTYRADVE
                     KIIAKKAKGQEITYEEPEEQPAGVIDLVAALQASLDAKKPRKAAKKAPARKRKAS"
BASE COUNT          469 a          725 c          760 g          373 t
ORIGIN      
        1 atcaccggaa cgcgatttcg gtccccgctc cgacgacgac gtctacatcc tctacaccgg
       61 tggcaccacc ggcatgccca agggtgtggt gtggcgccaa gaggatgtgt tgtgcgtgct
      121 cggcggtctg gtcgcgttcg acaccgggat acgggtttcg gacgagtggg agtgggcgaa
      181 ggcggcccag gcgcaggacg tgccgacgaa aagcgtcgtg attgcgccgc tgatgcacgg
      241 cgccgcgcag tggacggtcc tcaacggatt gctgaagggc accacgaccg tgatgctgcc
      301 caagttcgag ccggacgaag tgtggaaggc ggtcgacgcg caccaaattc cgacgatcgc
      361 catcaccggc gacgcgatgg gtcgcccgct gatcgaggcg ttcgaggccg gcgaatacga
      421 cggatcgtcg cttgtcaccg tcgcgtcgac ggcggcgatc ttcagcgcaa cggtgaagga
      481 tcggtacctc gaacagttcc caaacctgat catcgccgac gccgtcggct ccaccgagac
      541 cggttacaac ggcgtgcggg tgatcgtgaa aggcgtcgag cagccgctcg gcccgccgac
      601 gatcaacatg ggtccggaca ccgtcgtgct cgacgacgat ctcaacatcg tcgaacccgg
      661 ttccgatgtt gtcggccgga tggcgcgcgg cggaaacgtg ccactgcgtt accacaagga
      721 tccggaaaag tcggcggcca cattcgtgac ggccaagaac gggaagcgct attcgatccc
      781 gggcgacttc gcacgcgtcg aagccgacgg gtcgatgacg ctgctcggac gcggttcggt
      841 ctgcatcaac tccgggggcg agaaaatctt ccccgaggaa gtggagtcgg cactgaaggc
      901 gcatgtggcc gtcttcgacg cagtggtcgt cggtgtgccc gacgaacggt ggggcgaacg
      961 cgtcgcggca gtcgtcgaag tgcgcgtcga tgccacgatc acgctcgagg aactcgacgc
     1021 gcattcacgc gagctcatcg cgggctacaa agtgccgcgt gagctgcacg tcgttccgaa
     1081 gatcgaacgg tcgccgagcg gcaagcccga ctaccgctgg gccaagagcg tcgccctcgg
     1141 tgagatttag ctacttcaac tcgacgatga tctcgcggta ctgctgctcg ccgcagttga
     1201 ctgccgtctc gatgccgccg gccggcacga acgtgaccat gccggggatc acgtcggcgg
     1261 cgaagtagtc cttgtacgga cttgcagtgt cgggttcggg gtcgacggcg atgcggtcgc
     1321 cctgcacctg aatcaacagg tgatccagct cgtgctggtg gattgcgctg cgctcaccgg
     1381 gcgcgagctt cagaacccag acccgcaccc ggtcgtcctc gtagacgacc ttgctgccga
     1441 caccgcccag aacccggtcc tcgctcacat cacgaccgta acccctgggt agaaccgaag
     1501 tcatgcctcg ctcactcagg aatggctccg tctcgttcgg actggtcaac gtgccgatcg
     1561 cgctgtacac cgcgatcgag cccaaggaca tccacttcca tcagatgacc aagagcggtc
     1621 accgcattcg gcagaagcgc gtcgacgaga agaccggtcg cgaagtcgaa tacaagtcga
     1681 ttgtgaaggg ctacgaacag tcgaagggca agtacgtgat ggtcgagccc gaagagatcg
     1741 atgccgccgc gccgaagcag acgcgtgcca tcgagatcga ggagttcgtc gacctcgcgg
     1801 agatcgatcc gatctactac aactcgacgt actacgtcgc gccggggaag ggtgcgggcg
     1861 ccgacaaggg ttacgcgttg ttgcgcgatg tcatggagaa gagtggtcgc gccgcgatcg
     1921 gccgcttcgt catgcgtacg aagcagtacc tcgcggccat tcgtgtcgac aaaggcacgc
     1981 tcgtgctcca cacgctctac ttcgccgacg aaatccgcga caccaagggc ctcggcattc
     2041 cgtcgcgtac gaaggccggc gaccgagaag ttcgcatcgc gcgccagctc gtcgacagcc
     2101 tcacggtcga gtgggatccg aagcgtcaca aggacaccta ccgggccgac gtggagaaga
     2161 tcatcgccaa gaaggcgaag ggccaagaga tcacttacga ggagccggag gagcagcccg
     2221 cgggggtcat cgacctggtc gccgccctcc aggcgagcct ggatgcgaag aaaccccgca
     2281 aggcggcgaa gaaagcacct gctcgcaagc gcaaggcgag ctaactc
//
LOCUS       DASUGA010000797         7955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1863244, whole genome shotgun sequence.
ACCESSION   DASUGA010000797 DASUGA010000000
VERSION     DASUGA010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7955
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1863244"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VFX21_15665"
     CDS             complement(<1..348)
                     /locus_tag="VFX21_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX5097466.1"
                     /translation="MRDLRFAVIGAGMAGILAAIKLREAGYDDFTVYEKADSVGGTWR
                     ENTYPGLACDVPSHLYSYSFALDPEWSHRYSPGPEIRAYFERIAAEHDVIPRVRFGQE
                     VTRCAWVDGRWQLG"
     gene            432..626
                     /locus_tag="VFX21_15670"
     CDS             432..626
                     /locus_tag="VFX21_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX5097467.1"
                     /translation="MRVHVDRDKCQGHNRCYAIAPELFDVDDYGNAFELNDGNVPPAL
                     EDKARNAAANCPEYAIEITE"
     gene            631..1845
                     /locus_tag="VFX21_15675"
     CDS             631..1845
                     /locus_tag="VFX21_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5097468.1"
                     /translation="MTHTRPPVTDWAKDFDHTDPQWVANPFPIWDELRERCPVAHTDR
                     YGGAWLPVRHADVAAVAYDTEHFTSRSVVMSELRPGPDELPAPIGIAPPITSDPPFHQ
                     LARRMLLPAFSPKAIAALEAPTRELCRELLAATNGKEIVDAAVDYAQHIPLRVIVRML
                     GFPEEDADIFRRFIRMVLEDVDATEEERLAQIDDGELEDYINARIDDHIANPRDDLTS
                     FLIDAELDGNKLEPDHIRGTMVLLMIAGIDTTWSAIGASLWHLATHADDRRRLAAEPQ
                     LLDTAMEEFLRAYAPVTMARMVKEDFDFTGCPMKEGDWLLLPFPAANRDPEMFPDADD
                     VVIDRAENRHAAFGLGIHRCLGSNLARMELRVALEEWMRVYPEFTLVDAGAVTWSAGQ
                     VRGPRTIPVRVG"
     gene            complement(1852..2736)
                     /locus_tag="VFX21_15680"
     CDS             complement(1852..2736)
                     /locus_tag="VFX21_15680"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016884063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5097469.1"
                     /translation="MDFVQRVTREGGSDYVAPEQRIAVYDNDGTLWCEKPMPIQLGFL
                     LQRMAAMATDDPSLRDQQPYKAAVEKDFSWLGTAMDKHYRGDDGDVRLLMGAVLKAFG
                     GWTVDDYCASASAYLQSGRHPTLGRDLTACGYAPMVELLRYFEAHGFTNYIASGGDRD
                     FMRTISSEMYGIPPERVIGSSNSLQYVETDDGGSVVYGAEPDVFDDGPTKPVRIWSRV
                     GERPLVAFGNSNGDIPMLDYSDSPARGGLQLLLLHDDADREFDYTAGAEDALKRAADR
                     GWTVVSVKHDWSTVFAGA"
     gene            complement(2834..4165)
                     /locus_tag="VFX21_15685"
     CDS             complement(2834..4165)
                     /locus_tag="VFX21_15685"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5097470.1"
                     /translation="MDASHSRRTSEVKYARLGDAHIAYRVVAGRAPETRDSVLATAGT
                     VAMNSMFEDPMAMRFVDGLSELGRLVMFDRRGIGLSDPPEDWSVPVLTRWSEDIGAVI
                     SAAELHAPVIVTVRNAAAASLLYDAGAPGVASMLVMFEPAPFHNRAEGIQAQIDGEID
                     SVALFCPSRASEPGFREWFIRAGQTGASPAIAARAYPRYDDVQLAELARAGASVTAPL
                     VVLRRPGHRLSPAPEDDEVLALVPAATRVDIPGEDLLPFGNEVDTLLAEIARFVTGEH
                     RPPVAERSLVAVLYSDIVSSTDRAAALGDAHWKRLIDRHDAIARACISRRGGRVIKTM
                     GDGVLAVLGSASSAVLAADELRTALRAEDLEIRVGLHVGDVDRRGDDISGMAVVIAAR
                     LLALAAPGEILASMSVVVATTGEPIAYDARGEHTLKGVPGTWRAVAVTAHP"
     gene            complement(4218..4925)
                     /locus_tag="VFX21_15690"
     CDS             complement(4218..4925)
                     /locus_tag="VFX21_15690"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HEX5097471.1"
                     /translation="MPVDPDALDNVLAGLRGIDDPDLGVQVVLQRVVDAAQHLFALKG
                     AGLMFVDDGQALRYVAASDEPGRILEIAQEERGVGPCVDALVHEVVVTTRDIATDDRY
                     EAIAAIVVREGVRSVMGMPVRLGGTTIGSLNVYADQPTDWDDTDIAAIGALAEVIEAI
                     IGAAVLTHRQGNVVEQLEFALQNRVAIERAVGAIMNRDRVGPVEAFNVLRTEARSQRR
                     KVADVAAEVLLAVVDGR"
     gene            complement(4932..5240)
                     /locus_tag="VFX21_15695"
     CDS             complement(4932..5240)
                     /locus_tag="VFX21_15695"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5097472.1"
                     /translation="MSNDEVTVRLSGELDLSNRETLERALAQVSEERDRVVVLDLSGL
                     EFVEAHAVARWHVVGLHLRDRGSRLVLRHPRPVVERMMSLMDLDGAVEVDSSDPAREQ
                     "
     gene            complement(5417..6175)
                     /locus_tag="VFX21_15700"
     CDS             complement(5417..6175)
                     /locus_tag="VFX21_15700"
                     /inference="COORDINATES: protein motif:HMM:NF013341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HEX5097473.1"
                     /translation="MDTAWLIADPEPYEDYDLGPLKTGKEAEVFLVERVAPDGRSCLL
                     AHKRYRPRSVTHKGELQALGFQRAANFVNDQTYRDGLTIKNARDRRAVAKKTRHGREV
                     LRGNWPANEFAILSKLADAGVSVPFPVAQTEDGVLMQYIGDHDMAAPRLGTAGLARTD
                     LERATAQLVDNLVGMVEVGVVHADLSVYNLLWWDDRLWIIDVPQAVDISTNEQALDFL
                     HRDITNVGTWLRSRRVDFDSEELFARLVGVALGS"
     gene            6372..7370
                     /locus_tag="VFX21_15705"
     CDS             6372..7370
                     /locus_tag="VFX21_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005848861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5097474.1"
                     /translation="MPNPPHPMTDEWRALVDEPAIDPEQRIVDAHHHLWPPGGVLPYS
                     LGDLLADIGDGHRVERTVFMECGASYRTDAPPELAPVGETAFVARAAQECSGRIAGIV
                     GHADLRLANLDDVLDAHVAAGDGLFRGIRDALSRAEPGAGLMIPRGAPEGKFEDPAFR
                     AGVARLGARDLTYDSWHYHYQNHEFLELARAVPGTLMVLDHFGTPIGIGRWAEYRDAI
                     FEDWKHDIAEIARCENVVAKLGGLAMPDNGFGWHLAARPPTSDEFVAAQARYYLHTID
                     CFGPERCMFESNFPVDRMSLSYRVLVNGVKKILRTCSAEERDAIMFGTASRVYRLD"
     gene            7375..7920
                     /locus_tag="VFX21_15710"
     CDS             7375..7920
                     /locus_tag="VFX21_15710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5097475.1"
                     /translation="MTLRIETFTTEALDDARREAVIELNVAAHREEEFRQMFTKYIPS
                     GGRHLLGFLGAQLVTHAVVTTRFAQPHGAPILRTAYVDGVSTHPDHERQGYGSASMRR
                     LAASIDDYEIGCLQTDVRGFYEPLGWELWRGPLAGREDDGTVTPTPDQTGVMVLRLPN
                     SPPLDLDGLLTIERQQYRIWE"
BASE COUNT         1336 a         2782 c         2618 g         1219 t
ORIGIN      
        1 cccgagctgc cagcggccgt cgacccatgc gcagcgggtc acttcctggc cgaaccggac
       61 gcgcgggatc acgtcgtgtt cggcggcgat gcgctcgaag tacgcccgga tctcgggtcc
      121 gggcgaatac cggtgcgacc actccgggtc gagggcgaag gagtaggagt agagatgaga
      181 cgggacgtcg cacgcgaggc ccggataggt gttttcgcgc catgtgccac cgacgctgtc
      241 ggccttctcg tagaccgtga aatcgtcgta accggcttcg cgcagcttga tcgcggcgag
      301 gatccccgcc atcccggcac cgatcactgc gaaccgcaag tcacgcacga gcggtcaccg
      361 tagcgtgaac cacggcgcgc gagatccgga tcccgagcac taacgtaacc ggagcgaacg
      421 ggaggtgcgc ggtgcgagtc cacgtcgatc gcgacaagtg ccaggggcac aaccgctgct
      481 acgccatcgc acccgagttg ttcgacgtcg acgactacgg caacgcgttc gagctgaacg
      541 acggcaacgt gccgcccgcg ctggaagaca aagcccgcaa cgccgccgcg aactgtcccg
      601 agtacgcgat cgagatcacg gagtagcgcg atgacgcata cacggcctcc ggttaccgat
      661 tgggcgaagg acttcgacca caccgatccg cagtgggtcg cgaacccgtt cccgatctgg
      721 gacgagctgc gcgagcgctg cccggtcgcg cacaccgacc gctacggcgg cgcctggctg
      781 ccggtccggc acgccgatgt cgcggccgtc gcctacgaca ccgagcactt cacgtcgcgt
      841 tcggtcgtga tgagtgagct gcggcccggg cccgacgagc tgccggcacc gatcggcatc
      901 gcgccgccga tcacttcgga tcccccgttc caccagctcg cccggcgcat gttgttgccc
      961 gcgttctcac cgaaggcgat cgccgcactc gaagcaccca cccgcgaact gtgccgcgaa
     1021 ctgctcgcgg caacgaacgg caaagagatc gtcgacgcgg ccgtcgacta cgcgcaacac
     1081 atcccgctgc gcgtgatcgt caggatgctc ggcttcccgg aggaggacgc cgacatcttc
     1141 cgccggttca tccgcatggt gctcgaagac gtcgacgcga ccgaggaaga acggctcgcg
     1201 cagatcgacg acggcgagct cgaggactac atcaacgcgc gcatcgacga ccacatcgcg
     1261 aacccacgcg acgatctgac ctcgttcctc atcgacgccg agctcgacgg caacaagctc
     1321 gaacccgacc acatccgcgg cacgatggtg ctgttgatga tcgccggcat cgacacgacg
     1381 tggtcggcga tcggcgcgtc actgtggcac ctcgccacgc acgccgacga ccggcggcgg
     1441 ctcgcggccg aaccgcagct gctcgacacc gcaatggagg agttcctccg cgcgtacgcg
     1501 ccggtgacga tggcccggat ggtgaaggaa gacttcgatt tcaccggttg ccccatgaag
     1561 gaaggcgact ggctgctgtt gccgttcccg gccgccaacc gtgaccccga gatgttcccg
     1621 gacgcggacg acgtggtcat cgaccgggcc gagaaccggc acgccgcgtt cgggctcggc
     1681 atccaccggt gcctcggctc gaacctcgcg cggatggagc tgcgcgtcgc gctcgaggag
     1741 tggatgcgcg tgtacccgga gttcacgctc gtcgacgccg gcgcggtgac atggtcggcc
     1801 gggcaggtgc gcggcccgcg cacgatcccc gtacgcgtcg gctgacgcaa atcacgcgcc
     1861 cgcgaacacc gtcgaccagt cgtgcttgac gctgacaacg gtccaaccgc gatcggcggc
     1921 ccgcttcagc gcatcctccg cgccggccgt gtagtcgaac tcgcggtcgg catcgtcgtg
     1981 cagcaacagc agttggagcc cgccgcgcgc cgggctgtcg ctgtagtcga gcatcgggat
     2041 gtcgccgttc gaattgccga acgcgacgag cggccgctcg ccgactcggc tccagatgcg
     2101 caccggcttc gtcgggccgt cgtcgaacac gtcgggctcg gcgccgtaga cgaccgagcc
     2161 gccgtcgtcg gtctcgacgt attgcagcga attggaactg ccgatcacgc gctcgggcgg
     2221 aatgccgtac atctccgagc tgatcgtgcg catgaagtcg cggtcgccgc ccgatgcgat
     2281 gtagttcgtg aacccgtgcg cttcgaagta acgcagtagc tcgaccatcg gcgcgtatcc
     2341 acacgccgtg aggtcgcggc cgagcgtcgg atgccggccg gattggaggt acgcgctcgc
     2401 cgacgcgcag tagtcgtcaa cggtccaacc accgaacgcc ttcagcaccg cgcccatgag
     2461 cagccgcacg tcgccgtcgt cgccccgata gtgcttgtcc atcgcggtac cgagccacga
     2521 gaagtccttc tcgaccgcgg ccttgtacgg ctgctgatcg cgcaacgaag gatcgtcggt
     2581 cgccatcgcg gccatgcgct gcaacaggaa gccgagttgt atcggcatcg gtttctcgca
     2641 ccagagtgtg ccgtcgttgt cgtagacggc gatgcgctgc tcgggcgcga cgtagtcgga
     2701 tccaccttca cgcgtcacgc gctgaacgaa gtccatgatc gccgaacgag tggcaccgtc
     2761 gctccaggag ggaagtatgc gttcggtcac gcggccgacg gtatcgacac cgcgcgccgg
     2821 gtgccaccac cccctatggg tgagcagtca ccgcgacggc gcgccacgta cccgggacgc
     2881 ccttcagcgt gtgctctcca cgcgcgtcgt acgcaatcgg ctcgccggtc gtcgccacaa
     2941 caaccgacat cgacgccagg atctcacccg gcgccgcgag cgcgagcagc cgcgccgcga
     3001 tgaccacggc catgcccgag atgtcgtcgc cgcggcggtc gacgtcgccg acgtgcagtc
     3061 cgacccgtat ctcgagatcc tccgcccgca gcgcggtgcg gagctcgtcc gcggcaagca
     3121 cagcactgga tgccgaaccg agcaccgcga gcacgccgtc gcccatcgtc ttgatgacac
     3181 gcccgccgcg gcggctgatg caggcgcgcg cgatcgcgtc gtggcggtcg atgaggcgct
     3241 tccaatgcgc gtcaccgagt gcggcggccc gatcggtgga cgagacgatg tcgctgtaca
     3301 acacggccac cagcgatcgc tcggcgaccg gcggccggtg ctcgccggtg acgaaccgcg
     3361 cgatctccgc caacagcgtg tcgacctcgt tgccgaacgg gagcagatct tcgccgggaa
     3421 tgtcgacgcg agtggccgcc ggcacgaggg caagaacctc gtcgtcctcc ggtgccggac
     3481 tcaaccgatg acccggtcgg cgcaacacga ccagcggtgc ggtgacgctt gccccggcgc
     3541 gcgcgagctc ggccagctgc acgtcgtcgt agcgcggata cgcgcgtgcg gcgatcgcgg
     3601 ggctggcgcc ggtctggccc gcacggatga accactcccg aaatcctggc tcggacgccc
     3661 ggctcgggca gaacaaggcg accgagtcga tctcgccgtc gatctgcgcc tggatcccct
     3721 cggcgcgatt gtggaacggc gcgggctcga acatcaccag catcgacgcg acaccgggag
     3781 ctcccgcgtc gtacaacagc gaggcggcgg cggcgttgcg gaccgtcacg atcaccggcg
     3841 cgtgcagttc ggcggcactg atgaccgcgc cgatgtcctc gctccagcgc gtcaggacgg
     3901 gcaccgacca atcctcggga ggatccgaca acccgatgcc gcggcggtcg aacatcacga
     3961 ggcggccgag ctcggagagc ccgtcgacga agcgcatcgc catcgggtcc tcgaacatcg
     4021 agttcatcgc gaccgttccg gccgtcgcca acacgctgtc gcgcgtttcc ggggcacgcc
     4081 cggcgacgac gcgataggcg atgtgcgcgt caccgagccg ggcgtacttg acctcgctcg
     4141 tgcgccggga atgactcgcg tccatcgtgc ccagcctgcc cggcgcgccg cgcgtcgcgc
     4201 cacacgaagc gacgcgatca gcgcccgtcg acgaccgcca agaggacttc ggccgcgacg
     4261 tcggcaactt tgcgccgttg cgaccgcgcc tccgtgcgca agacgttgaa cgcctcgacg
     4321 ggcccgaccc ggtcacggtt catgatcgcg ccgacggcgc gctcgatcgc gacgcggttc
     4381 tggagcgcga actcgagctg ctcgacgacg ttgccttggc ggtgggtcaa gaccgccgcg
     4441 ccgatgatcg cctcgatgac ctcggccagg gcgccgatcg ccgcgatgtc ggtgtcgtcc
     4501 caatccgtcg gttgatcggc gtatacgttc agagagccga tcgtcgtgcc gccgaggcgc
     4561 accggcatgc ccatcacact ccgcacgccc tcgcgcacga cgatcgccgc gatggcctcg
     4621 tagcgatcgt cggtcgcgat gtcgcgcgtc gtcaccacga cctcgtgcac caacgcgtcg
     4681 acgcacggac cgacgccgcg ctcctcctgc gcgatctcga gtatccggcc cggttcatcg
     4741 gaagccgcga cgtaacgcag ggcctgcccg tcgtcgacga acatcaggcc ggcgcccttc
     4801 agcgcgaaca ggtgctgcgc ggcgtcgaca acgcgctgca acacgacttg aacgccgaga
     4861 tcgggatcgt cgatgccgcg cagaccggcc aggacgttgt cgagcgcgtc aggatctacg
     4921 ggcatgtccg ttcattgttc acgggccggg tcggacgagt cgacctcgac cgcgccgtcg
     4981 agatccatga gcgacatcat ccgctcgacg acgggtcgcg gatgtcgcaa cacgaggcga
     5041 ctgcctcggt ctcggagatg cagtccgacg acgtgccacc gggccacggc atgtgcttcg
     5101 acgaattcga ggcccgacag atcgaggacg acgacgcggt cccgttcctc ggagacctgc
     5161 gccaacgctc gctcgagcgt ctcgcggttc gacaggtcca actcgcccga caggcgcacg
     5221 gtgacctcgt cgttgctcac ggtcaacact gcatcgaaaa gatcgcttcc gaatcgctcg
     5281 ggcgtatctg gcttctccac cgaccctcca gttgtggcga cgaacgtcgc accgcggggt
     5341 catggcgggg cgctctccag agtgccagac aatcacgttg tgccggttcg gttctgaaaa
     5401 aggcctgaat gcccagctag gagcccaggg ccaccccgac caaccgggcg aacagttcct
     5461 cgctgtcgaa gtcgacccgg cgcgaccgca accacgtccc gacgttggtg atgtcgcggt
     5521 gcaagaagtc gagcgcctgt tcgttggtgc tgatgtcgac cgcttgcggc acgtcgatga
     5581 tccagaggcg atcgtcccac cacagcaggt tgtagaccga gaggtcggcg tgcacgacgc
     5641 cgacctcgac catgccgacg aggttgtcga cgagttgtgc ggtggcgcgt tcgaggtcgg
     5701 tacgcgccag tcccgccgtc cccagccgcg gcgcggccat gtcgtgatca ccgatgtact
     5761 gcatcagcac gccgtcttcg gtctgcgcga ccgggaacgg cacgctgaca ccggcatcgg
     5821 ccagcttcga caagatcgcg aactcgttcg caggccagtt cccgcgtagc acttcgcgac
     5881 catggcgcgt cttcttggcg accgcgcgcc gatcacgcgc gttcttgatc gtgagcccgt
     5941 cgcggtacgt ctggtcgttg acgaagttgg cggcgcgttg gaagccgagc gcttgcagct
     6001 cgcccttgtg cgtcacactg cgaggccgat agcgcttgtg cgccaacagg caggaacgac
     6061 cgtcgggtgc aacgcgctcg acgaggaaga cctctgcttc tttgcccgtc ttgaggggac
     6121 cgaggtcgta gtcctcgtac ggttcggggt cggcgatgag ccacgccgtg tccatgtgga
     6181 gctccagtgt cgggatccgg tggcgggact cccgactgga gcgtcaggcg atcgaaacga
     6241 ccgcgtcgcg agagccggga gtaggtgcag caacgatcgt cacggcttca cacctcctga
     6301 ctcggcggac ggcgcagagg atggccgctc ggccgcgagc gggcaagtga ttttcggtgt
     6361 atgacacggc cgtgccgaac ccgccccatc ccatgaccga cgaatggcgg gcgctggtcg
     6421 acgagcccgc gatcgatccc gagcagcgga tcgtcgatgc ccaccatcac ctgtggccgc
     6481 ccggcggcgt gctcccctac tcactcggcg atctgctcgc cgacatcggc gacgggcacc
     6541 gcgtcgagcg cacggtcttc atggaatgcg gcgcctcgta ccgcaccgac gcgccacccg
     6601 agctcgcgcc cgtcggcgag accgcgttcg tcgcgcgggc cgcacaggag tgcagcggac
     6661 gcatcgccgg catcgtcggt cacgccgacc tccggctcgc gaacctcgac gacgtgttgg
     6721 acgcgcatgt ggccgccggt gacgggttgt tccgcggtat ccgcgacgcg ttgtcacgcg
     6781 cggagcccgg ggccgggctc atgatcccgc gcggcgcgcc cgagggaaag ttcgaagatc
     6841 ccgcgttccg ggccggcgtc gcgcgactcg gtgcgcgcga tctcacgtac gacagctggc
     6901 actaccacta ccagaaccac gagttcctcg agctcgcgcg cgcagtgccg ggcacgctca
     6961 tggtcctcga ccacttcggc acacccatcg gcatcggccg gtgggcggag taccgcgacg
     7021 cgatcttcga ggactggaag cacgacatcg cggagatcgc gcgctgcgag aacgtcgtcg
     7081 ccaagctcgg cggtctcgcg atgcccgaca acggcttcgg ctggcacctg gccgcgcggc
     7141 cgccgacgtc ggacgagttt gtcgccgcgc aggcgcgcta ctacctgcac acgatcgact
     7201 gcttcggccc cgaacggtgc atgttcgagt cgaacttccc ggtcgaccgg atgtcgctct
     7261 cgtatcgcgt gttggtgaac ggtgtgaaga agatcctccg gacctgctca gctgaagaac
     7321 gcgacgcgat catgttcggc accgcatcgc gcgtctaccg actcgactga ctccatgacg
     7381 ctgcgcatcg agacattcac tacggaggca ctcgatgacg cacgtcgcga agcagtcatc
     7441 gagctgaacg ttgccgcgca tcgcgaggaa gagttccggc agatgttcac gaagtacatc
     7501 ccctcgggag gccggcacct gctcggcttt ctcggcgccc agctcgtcac ccacgcggtc
     7561 gtgacgacgc gcttcgcgca gccgcacggc gcgccgatct tgcggaccgc gtatgtcgac
     7621 ggcgtctcga cgcatcccga ccacgagcgg cagggctacg gcagtgcgtc gatgcgccgg
     7681 ctcgcggcgt cgatcgacga ctacgagatc ggatgcctcc agaccgacgt gcgcggcttc
     7741 tacgaaccgc tcggctggga gctgtggcgc ggtccgttgg cgggacggga agacgacggc
     7801 accgtgacgc cgacacccga ccaaaccggc gtcatggtgc tccggctccc gaactcaccg
     7861 ccgctcgacc tcgacggact cctgaccatc gagcgccagc agtaccgcat ctgggaatga
     7921 gccgggccgg tcagccgcgc gcggcgatgt acttg
//
LOCUS       DASUGA010000798         2539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_511400, whole genome shotgun sequence.
ACCESSION   DASUGA010000798 DASUGA010000000
VERSION     DASUGA010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511400"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VFX21_15715"
     CDS             complement(<1..558)
                     /locus_tag="VFX21_15715"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5097476.1"
                     /translation="MAIRVGVDVGGTFTKAIAFDLERGDVTAVEVVATTHDHADGVAA
                     GVVEVVGKLAAEVGAANIELVTHSTTQAVNALLEGDVAKVGLVGLARSPDLRKARKRT
                     VDARIELSVGRSLDMVREFVDVTSGLDDAVAAATVASLQQGGAQAIAVAEAFAPDDDT
                     NEHVIAAAARAAGLPVTTSADLTGLY"
     gene            complement(561..1853)
                     /locus_tag="VFX21_15720"
     CDS             complement(561..1853)
                     /locus_tag="VFX21_15720"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEX5097477.1"
                     /translation="MPTARVVRRAGAAIFFALVVVGLGASAASAQSPAVSRSEAITQL
                     DTVRESIDRTLALVKAGHSEEAFAEAKSGYLSHFEYVEIPLRVADAELTSDAETQFAE
                     IRGLISGGAPSSEVRDAIIELRGLIDDAERKLTDEGLSAPAVVFTQSFIIIFREGLEA
                     VLLVSVLLGYLEIAKARQYRRPILLGMAVAAGATIVTFFLLRGVLASLPFGREVLEAI
                     TALLATAVLFYVSFWLIARLEHKRWLEFLRARVWTAVSVGSTAALMLVGFTAVYREGF
                     ETALFYQALLSFGPGLGGWVLAGLAAGVVALGIVSWFIFRLGKRVPVKTFLSIAVVLL
                     MVTSIAFLGNAIHSLQEADVIGWHRWPGWPRAPIFLAQSLGYWPSRETITAQAALAMV
                     YVVGAFYVFVVKPQLDRRARVDARRGTERPAVAADAAH"
     gene            complement(1853..2275)
                     /locus_tag="VFX21_15725"
     CDS             complement(1853..2275)
                     /locus_tag="VFX21_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097478.1"
                     /translation="MRVLAGAVVGIALLAGCGSSSKSAEGDGPTTTVAPSPEDLRTSP
                     ALVAAGLKKIDTTVKELSTVTGTDKARAEELAEGIEKDWEPIEGTIRENDDEAYITFE
                     DQFAVLGKAAAEDDTARAKAAAAIVAKAVTDYLAAYPG"
     gene            2409..>2539
                     /locus_tag="VFX21_15730"
     CDS             2409..>2539
                     /locus_tag="VFX21_15730"
                     /inference="COORDINATES: protein motif:HMM:NF016230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5097479.1"
                     /translation="MYVCHCRAVTDTTIRTTIECGAEDVTDVGSMCGAGTNCGGCIS"
BASE COUNT          448 a          869 c          829 g          393 t
ORIGIN      
        1 gtacaaaccc gtgaggtcgg cggaggtcgt gacgggcaaa cctgccgcgc gcgccgcggc
       61 tgcgatcaca tgctcgttgg tgtcgtcgtc gggcgcgaac gcttcggcga ccgcgatggc
      121 ttgcgcgcct ccttgttgca gtgacgcgac cgtcgcggcc gcgaccgcgt cgtcgagccc
      181 gctcgtcacg tcgacgaatt cccgcaccat gtcgagcgag cggccgacgc tgagctcgat
      241 gcgcgcgtcg acggttcgct tgcgcgcctt gcgcaggtcg ggtgaccggg cgaggcccac
      301 cagtccgacc ttggcgacgt cgccttcgag caaggcgttg acggcctgcg tcgtggagtg
      361 cgtgaccaat tcgatgttgg ccgccccgac ctcggccgca agcttgccaa cgacctcgac
      421 gacgccggcc gcaacgccgt cggcgtggtc atgagtggtg gcaacgactt cgacagcagt
      481 gacgtcgccc cgttcaagat cgaaggcgat ggccttggtg aaggtgccgc cgacgtcgac
      541 gccgacgcgg attgccacgg ctagtgcgcc gcgtcggccg cgacggcggg gcgttcagtg
      601 cctcggcgcg cgtcaacacg agcgcggcga tcgagctgcg gcttcacgac gaacacgtag
      661 aacgcgccga cgacgtagac cattgcgagt gcagcctgcg cggtgatggt ctcccggctc
      721 ggccagtagc ccaacgactg cgcgaggaag atcggtgcgc gcggccaacc gggccaccga
      781 tgccagccga tcacgtcggc ctcttgcagc gagtggatgg cgttccccaa gaacgcgatc
      841 gacgtgacca tcaacagcac gaccgcgatc gacaggaacg tcttgaccgg cacacgcttg
      901 cccagacgga agatgaacca cgacacaatc ccgagtgcga ccacaccggc ggcgaggccg
      961 gcgagcaccc agccgccgag accgggaccg aaggaaagca acgcttggta gaacagcgcg
     1021 gtctcgaagc cctcgcgata gacggccgtg aacccgacga gcatcaatgc ggccgtcgag
     1081 cccaccgaca ccgcggtcca gactcgggca cgaaggaact ccagccaacg cttgtgctcg
     1141 aggcgcgcga tcaaccagaa cgagacgtag aacaagaccg cggtcgcgag caacgcggtg
     1201 atcgcttcga gtacctcgcg gccgaacggc agcgaggcaa ggacgccgcg gagcagaaag
     1261 aacgtcacga tggtcgcgcc tgcggcgacc gccatgccga gcaggatcgg gcgccggtat
     1321 tgccgggcct tcgcgatctc caggtagccc agcagcactg aaacgaggag taccgcttcg
     1381 aggccttcgc ggaaaatgat gatgaacgac tgagtgaaca cgaccgcggg cgcactcagc
     1441 ccttcgtcgg tgagcttgcg ctccgcatcg tcgatcaacc cgcgcagctc gatgatggcg
     1501 tcccgcactt ctgaactcgg tgcgccgccg ctgatgaggc cgcgaatctc ggcgaactgc
     1561 gtctcggcgt ccgacgtgag ctccgcgtcg gcgacgcgca gcggaatctc gacgtactcg
     1621 aagtggctga ggtagcccga cttcgcttcc gcgaacgcct cctcgctgtg gccggccttg
     1681 accagcgcga gcgtgcgatc gatcgactcc cgcacggtgt cgagctgcgt aatcgcctct
     1741 gagcgcgaga cggccggcga ctgggccgat gctgcgctcg cgccgagccc gacgaccacg
     1801 agcgcgaaaa agatcgcagc tccggcccgc cggacgacac gagcggtcgg catcagccgg
     1861 gatacgccgc gaggtagtcg gtgacggcct tcgcaacgat tgccgctgcg gccttggctc
     1921 gggcggtgtc gtcctcggcg gccgccttgc ccagcaccgc gaactgatcc tcgaaggtga
     1981 tgtacgcctc gtcgtcgttc tcgcgaatgg tgccttcgat cggctcccaa tccttctcga
     2041 tgccctcggc cagttcttcg gcccgggcct tgtcggtgcc ggtgacggtc gagagttcct
     2101 ttaccgtggt gtcgatcttt ttcagcccgg ccgccacgag cgccgggctc gtccgcagat
     2161 cctcaggcga cggtgccacc gtcgtcgtcg ggccgtcacc ttcggcactc ttcgagctgc
     2221 ttccgcagcc cgccagcagg gcgatgccga ccaccgcgcc cgccagcacc cgcaccctga
     2281 cctgcctcat cgcaaccatc ctcgagaaat atgttaggtg aacctaatac atccttgacg
     2341 ggatgcctaa gtcgggttta ccttacctga gagtgatgtg ttcaaccacg gcgcagaact
     2401 gaggcccggt gtacgtctgc cactgccgcg cggtcaccga cacgaccatc cgcaccacga
     2461 tcgagtgcgg cgccgaggac gtcaccgacg tcggctcgat gtgcggcgcc ggcaccaact
     2521 gcggcggttg catcagcga
//
LOCUS       DASUGA010000799         2233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_511417, whole genome shotgun sequence.
ACCESSION   DASUGA010000799 DASUGA010000000
VERSION     DASUGA010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511417"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VFX21_15735"
     CDS             complement(<1..444)
                     /locus_tag="VFX21_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014443137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX5097480.1"
                     /translation="MTEPAHARPRTSLRDVFVQWARIGCIGFGGPPTHIALLRELCVT
                     KRKWMTPDEFEDSIAACNLLPGPASTQLSILCAWNVAGPAGAVVGGLAFVVPGLAAIL
                     ALAAVFLAASPPTWIVAAGAGAGAAVAAVAVHAATSLVPASWERAT"
     gene            complement(466..852)
                     /locus_tag="VFX21_15740"
     CDS             complement(466..852)
                     /locus_tag="VFX21_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018012923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5097481.1"
                     /translation="MPDDEMVSVRYMVDDVDKAIDFYTRLLDFEVQMSAAPAFADVKR
                     GRLRLLLSGPKSSAGLAMEDGAQPGPGGWNRIHFIVDDLDREVTRLRDAGAQFRNPAV
                     EGPGGKQILLQDPSGNIVELFQPAAR"
     gene            complement(839..1615)
                     /locus_tag="VFX21_15745"
     CDS             complement(839..1615)
                     /locus_tag="VFX21_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003988034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5097482.1"
                     /translation="MPTEPPAFVQLLAHPVRWALLEELTRSDRAVRELTQLVDEPQNL
                     VSYHLRLLKDGGLVSSHRSSADGRDSYYAIDLAHCRDAMQATGGALHPGLWLVPAPAA
                     PVRPSGRQPRVLFLCTGNSARSQIAEALLADMSNHAVDVASAGSAPKPLHPNAVRVLR
                     TRGIDISQNRTKHFDELARRRFDVVVTLCDHVREVCPEYPHSTRMVHWSIPDPALEGP
                     TNRASYPAFGRTAAELETRIGFLLAQLHDPPSRRLQHARR"
     gene            1848..>2233
                     /locus_tag="VFX21_15750"
     CDS             1848..>2233
                     /locus_tag="VFX21_15750"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX5097483.1"
                     /translation="MKAVRFREPKGIEGIDGLVYEDAPDPKPAIGDALVQVRAASFTP
                     TELLWPLATDRHGHERGWRIPAHEGSGVVVELGYGAAGLAVGDEVYGLIDGYRDGWAA
                     EFVAIEVRSLAKKPASVDFVAAAAIP"
BASE COUNT          351 a          762 c          776 g          344 t
ORIGIN      
        1 cgtcgcgcgt tcccagctgg cgggcaccag actcgtcgcc gcgtgcactg ccaccgcggc
       61 gaccgccgcg cctgctcccg cgcccgcggc cacgatccac gtcggtggcg acgcggccag
      121 gaagacggcg gcgagcgcga ggatggcggc cagtcccggg acgacgaagg ccaggccgcc
      181 gacgaccgca cctgcgggtc cggcgacgtt ccatgcgcac aggatcgaga gctgtgtcga
      241 cgccggtccg ggcagcaggt tgcaggccgc gatcgagtcc tcgaactcgt cgggcgtcat
      301 ccacttgcgc ttggtgacgc acagttcgcg cagcaacgcg atgtgtgtcg gtggaccacc
      361 gaacccgatg caaccgatgc gggcccactg cacgaacaca tcgcgcagtg acgttcgcgg
      421 gcgcgcgtgc gccggttcgg tcatcggcgc tcgtgcggtg tcgaactacc gagccgccgg
      481 ttggaagagc tcgacgatgt tgcccgacgg gtcttggagc aggatctgct tcccgccggg
      541 accttcgacc gccgggttgc ggaactgcgc gcctgcatcg cgcagacgcg tgacctcgcg
      601 gtcgaggtcg tcgacgatga agtggatgcg gttccagccg ccgggcccgg gctgcgcgcc
      661 gtcttccatg gcgaggccgg cggagctctt ggggcccgac agcagcagcc gaagccggcc
      721 gcgtttgacg tcggcgaacg cgggcgccgc gctcatctgc acctcgaagt cgagcagccg
      781 cgtgtagaag tcgatcgcct tgtcgacgtc gtcgaccatg taacgcacgc tgaccatctc
      841 atcgtcgggc atgttgcaac ctcctgctcg gtgggtcgtg cagctgcgcc agcagaaagc
      901 cgatgcgggt ctccagctcg gcggcagtgc gtccgaaggc gggatacgac gcgcgattcg
      961 tcggtccttc gagtgcggga tccggaatgc tccaatgcac cattcgcgtc gagtgcggat
     1021 attcggggca gacctcgcgg acgtgatcgc acagtgtcac gacgacgtcg aagcggcggc
     1081 gcgccagctc gtcgaaatgc ttcgtgcggt tctggctgat gtcgatcccg cgcgtgcgca
     1141 gcactcgcac ggcgttcggg tgcagtggct tcggggcact gcccgcgctc gcgacgtcga
     1201 ccgcgtgatt cgacatgtcg gccagcaagg cttccgcgat ctgtgatcgc gcgctgtttc
     1261 ccgtgcacaa aaacagcact cgcggttgcc gccccgacgg gcgcaccggc gcggctggtg
     1321 ccgggaccag ccacaacccc gggtgcaacg caccacccgt cgcctgcatc gcgtcgcggc
     1381 aatgcgcgag atcgatcgcg tagtagctgt cgcggccgtc ggccgagctg cgatgcgacg
     1441 agacgagacc gccgtccttc agcagccgca ggtggtacga cacgaggttc tgcggctcgt
     1501 cgacgagctg cgtcagctcg cgcacggcgc ggtcgctgcg ggtcagctcc tcgaggagcg
     1561 cccaccgcac ggggtgggcc agcaactgca cgaaagccgg cggctccgtt ggcacgcccc
     1621 gaaccctacg cgactcgtac ccaatccatc aagcacattt gatgtattcc ggcactcgtc
     1681 atgcgcgagc agtgcccccg ccgcgaaggc ggctttgacc cgtcgaaccc accgtccggg
     1741 gaagtcctga ccggtcgcgc ggttgcacag ggagatgtcg atcccgcgcg acgccgttcg
     1801 tcgtgtgagg aaccgacgag acaatcccgc tgtgagagga gacttccatg aaggcagttc
     1861 gcttccggga acccaaaggc atcgagggca tcgacggcct tgtctacgag gacgcacccg
     1921 acccgaagcc cgccatcggc gacgcgctcg tgcaggtgcg cgccgcgagc ttcacgccga
     1981 ccgagctgtt gtggccgttg gcgaccgatc ggcacggaca cgagcgcggc tggcgcatcc
     2041 ccgcccatga agggtcgggc gtcgtcgtcg agctcggcta cggcgcggcc ggactcgcgg
     2101 taggcgacga ggtgtacggc ctgatcgacg gctatcgcga cggttgggcg gccgagttcg
     2161 tcgcgatcga ggtccgcagc ctcgccaaga agccggccag tgtcgacttc gtcgcggcgg
     2221 ccgcgatccc gca
//
LOCUS       DASUGA010000800         3460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_788514, whole genome shotgun sequence.
ACCESSION   DASUGA010000800 DASUGA010000000
VERSION     DASUGA010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788514"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2257)
                     /locus_tag="VFX21_15755"
     CDS             complement(<1..2257)
                     /locus_tag="VFX21_15755"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5097484.1"
                     /translation="MTPWPLVGRDAELALFEQLVLGDRRGIVLVAPAGTGKTRLVHET
                     MRRLERVAATETIAATRSAQSLPLASVAALLPEQTPAATEPLDLFRAVRRTLAARAGE
                     RPLVVAIDDAHLLDPLSAALVHHLALAGSVRFLIAIRSGEPVEDAITALWRDGIHERV
                     DMQPLGPDDVTSLLRAVLGGDVEAASAWRLWRITGGNPLYLREIVTEAQRAATLVDVA
                     GVWQWRGAVRVGVRLREIVEARLAGLDVAERAVVALLAVGERVAAGTVESVCDPNALA
                     ALERRGFVVAEPDGGRDRPAVYRLDHPLFAEAIRRSMPATERGRWCRVLADAAETTAP
                     DDTSLLQLTMWQLDGGLPIDASTLTYASERASARFDGALGERLADAAVDAGADDSARL
                     VRAEARLLQGRYADALDDAHAVTSVVFDDASLARLATVIAQAGFWGLGLVQETEDALQ
                     KIAGHVESIAARQRVLALESAMQLAAGRLPVAAELGLSIASDPAADALARLRAVTAAA
                     AGLSLRGHPDQALALCEELLPVALAHTDALPRGPGWVLAQTLFGLSCLGRFDGIAQLL
                     TPFREAAMAEGDEEVVHSGGVVIVRLALTAGDLEGARAAARETAAALRRYDPAGYLPW
                     CLGMLAQSAAQMGDVAGAQEAVAELDDLTWVVRLNDHDVAIGRAWAAAAAGEVTRPLA
                     ILFEAAGVARAAGNVFSEGLLLYEALRIGAHTRDLVDRLDACRAGGRLPVHEMFHARA
                     VASAANDGVALD"
     gene            complement(2260..3429)
                     /locus_tag="VFX21_15760"
     CDS             complement(2260..3429)
                     /locus_tag="VFX21_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HEX5097485.1"
                     /translation="MTTRGLAGRAAIAGIGQTEFSKESGRTELQLACEAVLAAIDDAG
                     VDAADVDGLVTFTMDTSEEMEIARNLGLREVSMFTRVPYGGGASAAVVMQAAMAVATG
                     VADNVVCYRAFNERSGNRFGTASAQVTTLPLWFAWYAPFGLMTPAAWVGLHARRYMET
                     YGVTNADFGRIAVIDRSHAATNPGAWFYERPITLEEHQASRWIVEPVLRLLDCCQESD
                     GGVALLITSTERARDLRQAPAVITAAAQGATFDSEMMTSYYRDDLTGLPEMGVVGRRL
                     WEMSGLAPADMSTAFLYDHFTPFVLMQLEELGFCQPGEAKDWATVERLSLGGDLPINT
                     GGGLLGEAYIHGMNGITEVVRQIRGTSCNQVPDVEHALVTAGTGVPTSGLILSKE"
BASE COUNT          508 a         1263 c         1213 g          476 t
ORIGIN      
        1 cgtcgagtgc gacgccgtcg ttcgcggccg acgcgacggc gcgtgcgtgg aacatctcgt
       61 gtacgggcag ccgtccaccc gcccggcacg cgtcgagtcg gtcgacgagg tcgcgcgtgt
      121 gcgcaccgat gcgcagcgcc tcgtacaaga gcaggccttc ggagaacacg tttccggctg
      181 cgcgcgcaac accggccgcc tcgaacagga tggcgagcgg tcgagtcacc tcgccggccg
      241 cggccgcggc ccacgcgcgc ccgattgcga cgtcgtggtc gttcagccgc accacccacg
      301 tcaggtcgtc gagctccgcg accgcttctt gcgcgccggc gacgtctccc atctgcgcgg
      361 cggactgcgc cagcatcccg aggcaccacg ggaggtaccc ggcagggtcg taacggcgca
      421 gcgcggcagc cgtctcgcgt gcggcggcgc gcgcgccttc gaggtcgccc gccgtgagcg
      481 ccagccgaac gatcacgacc ccgccgctgt gcaccacctc ctcgtcgcct tcggccattg
      541 cggcctcgcg gaacggcgtg aggagctgtg cgatcccgtc gaaacgtccg agacagctga
      601 gcccgaacag tgtctgcgcg agtacccaac cgggaccgcg cggtagcgcg tcggtgtgcg
      661 cgagggcgac cggaagcagc tcctcgcaca gtgcgagtgc ctggtcgggg tgaccgcgca
      721 aactgagtcc cgccgccgca gccgtgaccg cgcgcaggcg cgcgagcgcg tcggcagccg
      781 gatcggacgc gatcgacagg ccgagctccg ccgcgaccgg gaggcggccg gcggcgagtt
      841 gcatggcgga ctcgagcgcg agcacgcgtt ggcgggccgc gatgctctcg acgtggcccg
      901 cgatcttctg gagtgcgtcc tcggtctcct gcaccagccc gaggccccag aagcccgctt
      961 gggcgatcac ggtggcgagg cgcgcgagcg acgcgtcgtc gaacaccacg ctggtcacgg
     1021 catgcgcatc atcgagcgcg tcggcatatc ggccttgcaa caatcgtgcc tcggcgcgca
     1081 ccaagcgggc gctgtcatcg gcgccggcgt cgaccgccgc gtctgccagc cgttcaccga
     1141 gcgcgccgtc gaagcgcgcg ctcgcccgtt cgcttgcgta ggtgagtgtg cttgcgtcga
     1201 tcgggagccc gccgtcgagc tgccacatcg tgagctggag cagcgacgtg tcgtcgggcg
     1261 cggtcgtctc cgcggcatcg gcgagcacgc ggcaccaacg cccgcgctcg gtcgccggca
     1321 tcgaccgccg gatcgcctcg gcaaacagcg ggtggtccag gcgatacacg gcgggccggt
     1381 cgcggccgcc gtccggctcg gccacgacga aaccccgtcg ctccagggcc gccagcgcat
     1441 tcgggtcgca caccgattcg acggtgccgg ccgcgacccg ctcgccgacg gccaagagcg
     1501 cgacgaccgc gcgttcggca acgtcgaggc cggcgaggcg cgcctcgacg atctcccgca
     1561 gccgcacacc gacgcggacc gcgccccgcc actgccacac accggcgacg tcgacgaggg
     1621 tcgcggcccg ttgcgcctcc gtgacgatct cgcgcaggta cagcgggttg ccaccggtga
     1681 tgcgccagag ccgccacgca ctcgcggcct cgacgtcacc gccgagcacg gcccgcagga
     1741 gcgacgtgac gtcgtccggt ccgagcggct gcatgtcgac gcgctcgtgg atgccgtcgc
     1801 gccagagcgc ggtgatcgcg tcctcgaccg gctcgcccga acggatcgcg atcaagaagc
     1861 gcacactgcc cgcgagtgca aggtggtgga cgagcgcggc cgagagcgga tcgagcaggt
     1921 gcgcgtcgtc gatcgcgacg acgagcggtc gttcaccggc gcgcgccgcg agcgtgcgcc
     1981 ggacggcgcg gaacaggtcg agtggttccg ttgccgccgg cgtctgctcg ggcaacaacg
     2041 cggcgaccga cgccaggggc aacgactgcg ccgaccgggt ggcggcgatc gtctccgtcg
     2101 cggccacccg ctcgagccgc cgcatcgttt cgtggacgag ccgcgtcttg ccggtacccg
     2161 ccggggcgac gagcacgatg ccgcggcggt cgccgagcac caactgctcg aagagtgcga
     2221 gctcggcgtc gcgccccacg agcggccacg gtgtcatcgt cattccttcg acaggatcag
     2281 cccgctcgtg ggaacgccgg tgcccgcggt gacgagcgcg tgctcgacgt cgggcacttg
     2341 gttgcacgac gtgccgcgga tctgacgaac gacttcggtg atgccgttca tgccgtgaat
     2401 gtacgcctcg ccgagcaggc cgccgccggt gttgatcggg aggtcgccgc cgagcgacag
     2461 ccgttcgacg gtcgcccagt ccttcgcctc gcccggctgg cagaagccga gctcctcgag
     2521 ctgcatgagc acgaacggcg tgaagtggtc gtacaggaac gcggtcgaca tgtccgcggg
     2581 cgcgagccct gacatctccc acaagcggcg cccgacgacg cccatctctg gtagtccggt
     2641 caggtcgtcg cggtagtagc tcgtcatcat ctcgctgtcg aacgtcgcac cctgtgcagc
     2701 cgcggtgatg accgccggtg cctggcggag gtcgcgggcg cgttcggtcg acgtgatgag
     2761 cagcgcgaca ccgccgtcgc tctcctggca gcagtcgagc aaccgcagca ccggctcgac
     2821 gatccagcgt gacgcctgat gctcctcgag cgtgatcggc cgctcgtaga accacgcgcc
     2881 gggattcgtc gccgcgtgcg agcggtcgat cacggcgatg cgcccgaagt cggcgttggt
     2941 cacgccgtag gtctccatgt accggcgcgc gtgcaacccc acccacgcgg ccggcgtcat
     3001 gagtccgaac ggggcgtacc acgcgaacca cagcggcaac gtcgtgactt gggcgctcgc
     3061 ggtcccgaag cggtttccgg accgttcgtt gaaggcgcgg taacagacga cgttgtcggc
     3121 gacgcccgtc gcgaccgcca tcgcggcctg catcacgacc gcggcagatg caccgccgcc
     3181 gtagggcacg cgcgtgaaca tcgacacctc tcgcaaaccg aggttgcgcg cgatctccat
     3241 ctcttcgctc gtgtccatcg tgaacgtcac gagcccgtcg acgtcggccg cgtcgacgcc
     3301 cgcgtcgtcg atcgcggcga ggacggcttc gcacgcgagc tgcaactcag tgcggcccga
     3361 ctccttcgag aactcggtct gcccgatgcc ggcgatcgcg gcgcggcccg ccaatccccg
     3421 agtcgtcatg ccggaagact cagccgcgcg gtgccgccga
//
LOCUS       DASUGA010000801         3274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1205330, whole genome shotgun sequence.
ACCESSION   DASUGA010000801 DASUGA010000000
VERSION     DASUGA010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205330"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VFX21_15765"
     CDS             <1..471
                     /locus_tag="VFX21_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097486.1"
                     /translation="QPVMLARDISEEIDMDPDRVVLATQTPLGEGIPAWLQVCDAATA
                     EERRRSWRRRDYPTIVAVSLPPGREHLRWARAMLDSLEPTSTWAIVEAGWKLEDVRHW
                     AEVLGGIDVLALRNLAQTVSPAAALELGIPVARLEGQPATPIAWADYLMALARL"
     gene            481..780
                     /locus_tag="VFX21_15770"
     CDS             481..780
                     /locus_tag="VFX21_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097487.1"
                     /translation="MRRPTVDPAFTFAIALVMSLVLWWGTLKALLDGNVDITVAGLRY
                     LAALALSWCGVYFVASIVAMYASQPRPAPPEPETPQRRKTDAAQVSEPDAEAPAA"
     gene            complement(781..1272)
                     /locus_tag="VFX21_15775"
     CDS             complement(781..1272)
                     /locus_tag="VFX21_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097488.1"
                     /translation="MCVPSHYPVPTNEAIRAILADIVGGRAVTVALSQDPADTDAPGV
                     LADYSVDDGPVGVLCVADLSLSNVVGAAIAAETSEQVDAAVNDNQIADATIENWNEVV
                     NQIARLFNSPDTPTLRVREVRRLPAELPNDVNELLATPLARRAFDVEVADLGKGTLAV
                     VVG"
     gene            complement(1347..1622)
                     /locus_tag="VFX21_15780"
     CDS             complement(1347..1622)
                     /locus_tag="VFX21_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX5097489.1"
                     /translation="MARAISPTRTDWQAEAACRDVDTDIFFPVSDADAGPAIEICANC
                     PVREACLEWALEFRPSDGIYGGLTPIERHRLIRRRQKAERKARKDAA"
     gene            complement(1672..2199)
                     /locus_tag="VFX21_15785"
     CDS             complement(1672..2199)
                     /locus_tag="VFX21_15785"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5097490.1"
                     /translation="MNDPAWRSVSHMALVTPDMDTTVRFYAGVLGMPVVGAIMAGPMR
                     HYFFKIGEHNTVAFFEIPGMETWAKGAGSPPDRPIQFDHLAFDVADEDELLRLRKRLL
                     EHGCEVTEVVDHGMLQSVYFTDPNGIALEACYWVEDPTPAGADPIIEDPDPVPALREI
                     RETGRLRETPTTRLV"
     gene            complement(2208..>3274)
                     /locus_tag="VFX21_15790"
     CDS             complement(2208..>3274)
                     /locus_tag="VFX21_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX5097491.1"
                     /translation="LGGLTAVMLGDGYKPHEAPTPDTNVVLHAVDIDAPKPFRRKNAP
                     TFVLAVAQTASPPDDLLRTGYPLLVRALANVTIIVSDTPTGLAVRFVTLEQGTYGVGP
                     GLSDDELCARVFERLEPLMSSRLVIANDFVADLPEHLWDGDATTAQMLAAGRALDDLD
                     LLPAPFPLEEILGPRELRHVALLYGIGGLSYGNVSARYQGRDVAGVPDADGPVYWMSA
                     SGVDKSDLRVVGEHILLVRGYDAERDVMVLSVPPAITPRRVSVDAIEHWMIYREHPSV
                     GAILHVHGWIDGTTSTDVNYPCGTVQLAGAVADLVRAARDPARAVVGLRNHGLTITGE
                     SLDEIFARVGSRIVAQVPMS"
BASE COUNT          502 a         1121 c         1145 g          506 t
ORIGIN      
        1 caaccggtga tgctcgcccg tgacatctcc gaggagatcg acatggaccc cgatcgcgtc
       61 gtgctcgcga cgcaaacacc gctcggcgag ggcatccccg cgtggttgca ggtttgtgac
      121 gccgcgaccg ccgaggaacg ccgccggtcg tggcgccgcc gcgactaccc gacgatcgtg
      181 gcggtgagtc tgccgcccgg ccgcgagcac ttgcggtggg cgcgcgccat gctcgacagc
      241 ctcgagccga cgtcaacgtg ggcgatcgtc gaggccggtt ggaagctcga ggacgtccgc
      301 cactgggccg aggtactcgg cggcatcgac gtcctcgcgc tgcgcaacct cgcgcagacg
      361 gtcagccccg cggctgcgct cgagctcggc atccccgtcg cgcgcctcga aggccagccg
      421 gccacgccga tcgcgtgggc cgactacctg atggccctgg cgaggctcta ggagatcgcg
      481 atgcggcgcc cgacggtcga ccccgcgttc acgttcgcca tcgcactggt gatgtcgctg
      541 gtgctgtggt ggggcacact caaagcgttg ctcgacggca acgtcgacat cacggtcgcg
      601 ggcctgcggt acctcgcggc gttggctctc tcgtggtgcg gcgtctactt cgtggcgtcg
      661 atcgtcgcca tgtacgcgtc ccagccacgc cccgcgccac cggaaccgga aaccccccaa
      721 cgccgcaaga ccgatgcagc gcaagtatcc gagccggacg cagaagcacc agccgcgtaa
      781 ctagccgacg acgacggcga gggtgccttt gcccaagtcg gcgacttcga cgtcgaacgc
      841 gcgccgggcc aacggcgtcg cgagcagctc gttgacgtcg ttcgggagct cggcggggag
      901 acggcgcact tcgcgcacgc gcagcgtcgg ggtgtcggga ctgttgaaga ggcgcgcgat
      961 ctggttcacg acctcgttcc agttctcgat ggtcgcgtcg gcgatctggt tgtcgttgac
     1021 cgcggcgtcc acctgttcgg acgtctccgc cgcgatcgcc gcaccgacga cattgctcaa
     1081 cgacaggtcg gcgacacaca gcacaccaac cgggccgtcg tcgacgctgt agtcggcgag
     1141 cacgcccggc gcgtcggtgt cggccgggtc ctggctgagc gccacggtca ccgcgcggcc
     1201 gccgacgatg tcggcgagga tcgctcggat cgcctcgttc gtggggaccg gatagtgcga
     1261 gggcacacac aagattcggg tcgtccgccg atcgtcttga ggttacgaac ggcacaaact
     1321 cgatggcatt tccggagcac cgcagctcag gcggcgtctt tgcgggcctt gcgctcggcc
     1381 ttctggcgcc ggcggatcag gcggtggcgc tcgatcggcg tcagaccgcc gtagatcccg
     1441 tcggaaggcc ggaactccag cgcccactcc aggcaggcct cccggaccgg gcaattcgcg
     1501 cagatctcga tggccgggcc cgcgtcggca tccgacaccg gaaagaagat gtcggtgtcg
     1561 acgtcgcgac aggccgcctc ggcctgccag tcggttcggg tggggctaat tgcgcgtgcc
     1621 attggaagtg ttacaaccgc gggcccgcgg ccttgattcc cgggaaatcc cctagaccag
     1681 gcgggtcgtg ggggtctcgc gcagcctccc ggtctcccgg atctcccgca gcgcgggcac
     1741 cgggtcgggg tcctcgatga tcggatcggc gcccgccggc gtcgggtcct cgacccagta
     1801 gcacgcctcg agcgcgatgc cgttcgggtc ggtgaagtac acggactgga gcatcccgtg
     1861 gtcgacgacc tccgtgacct cacagccgtg ctcgagcagc cgcttgcgca gccgcaacag
     1921 ctcgtcctcg tcggcgacgt cgaacgcgag atggtcgaac tggatcggcc ggtcgggcgg
     1981 cgaccccgcg cccttggccc acgtctccat gccggggatc tcgaagaacg cgacggtgtt
     2041 gtgctcgccg atcttgaaga agtagtgccg catcggcccg gccatgatcg cccccacgac
     2101 cggcatcccg agcacacccg cgtagaagcg aacggtggtg tccatgtcgg gtgtcaccaa
     2161 tgccatgtgg ctgacgctgc gccacgcggg atcgttcacg gccactccta gctcatggga
     2221 acttgcgcca cgatacggct cccgacgcga gcgaagatct cgtcgaggct ctcgccggtg
     2281 atcgtgaggc cgtggttgcg cagcccgacg accgcgcggg ccgggtcgcg cgcggcccgt
     2341 acgaggtcgg ccaccgcgcc ggccagctgc accgtgccgc acgggtagtt gacgtcggtg
     2401 gacgtcgtgc cgtcgatcca gccgtgcacg tgcaagatcg cgccgacgct cgggtgctcg
     2461 cggtagatca tccaatgctc gatcgcgtcg accgagaccc gccgcggcgt gatcgcgggc
     2521 ggcacgctca acaccatgac gtcgcgctcg gcgtcgtagc cgcgcacgag gagaatgtgc
     2581 tcgccgacga cccgcagatc ggacttgtcg acaccgctgg cgctcatcca gtacacgggc
     2641 ccgtcggcgt cggggacgcc ggcgacgtcg cggccctggt agcgcgcgct gacgttgccg
     2701 tacgagagcc cgccgatgcc gtagagcagt gcgacgtgcc gcagttcgcg cggaccgagg
     2761 atctcctcga gcgggaacgg tgccggcaac aagtcgaggt cgtcgagcgc gcgtcccgcg
     2821 gcgagcatct gcgcggtcgt cgcgtcgcca tcccagaggt gctcgggaag atcggcgacg
     2881 aagtcgttcg cgatgacgag acgcgacgac atcagcggtt ccagccgttc gaacacgcga
     2941 gcgcacaact cgtcgtcgga gagaccgggg ccgacgccgt acgtgccttg ctccaacgtg
     3001 acgaagcgca ccgcgagacc ggtcggcgtg tcgctgacga tgatcgtgac gttggcgagc
     3061 gcgcgcacga gcagtggata gcccgtgcgc aacaagtcgt cgggcggcga tgcggtttgc
     3121 gcaaccgcga gcacgaacgt cggcgcgttc ttgcgccgga acggcttggg cgcgtcgatg
     3181 tcgactgcgt ggaggacgac gttggtgtcg ggcgtcggcg cctcgtgcgg cttgtacccg
     3241 tcgccgagca tcaccgcggt gagcccgccg agga
//
LOCUS       DASUGA010000802         3860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_270544, whole genome shotgun sequence.
ACCESSION   DASUGA010000802 DASUGA010000000
VERSION     DASUGA010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270544"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VFX21_15795"
     CDS             complement(<1..668)
                     /locus_tag="VFX21_15795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5097492.1"
                     /translation="MHQNYMRDYEAMKLRSVHDDAMQFSNPGPDAGGRGRFAGRVAAI
                     TGAGSGIGRALAEDLARRGAHLALCDVDEAGLAATVTRCEGVGVKVTSQRVDVADRAA
                     VEAWADQVVADHGRVNFIFNNAGVGLGATVANMTYDDFEWLMNINFWGVVHGTQAFLP
                     HLKSSGDGHVVNISSIFGLVSVPTQSAYNAAKFGVRGFTDALRIELETDPCGVSSTTV
                     HPGG"
     gene            complement(645..2039)
                     /locus_tag="VFX21_15800"
                     /pseudo
     CDS             complement(645..2039)
                     /locus_tag="VFX21_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020609181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
     gene            complement(2085..3137)
                     /locus_tag="VFX21_15805"
     CDS             complement(2085..3137)
                     /locus_tag="VFX21_15805"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX5097493.1"
                     /translation="MALFGSIALVGGLAFASPAAAAGHVVRPGDSIQAAIDDASPGDT
                     IILQAGTYHENIRIATDDITIIGSGATLMPPAAPVENECGDPEDPSVVSGVCIIGSDI
                     DFDTGAVGDPIDGVRIIGLHVEGFEGIGIVQIGGTDLSMIANTLVDNGEYGTAAFMSS
                     GTKAIANKVDGAEEAGLYFGDSPDADAKIIGNDTTDALFGIFVRNAEGAHIVGNRVHG
                     NCLGVIFLGDAPGPVGASDVFGNFIFENNRFCPPSEDTPFPIQGVGTALVGAHDVTLT
                     GNIITNNNGDSPVAGGVTVIAGGDPSAPGTPPTNNTVRGNIITNNTPDINWDGSGTGN
                     VFEHNVCDTSTPDGLC"
     gene            complement(3234..3695)
                     /locus_tag="VFX21_15810"
     CDS             complement(3234..3695)
                     /locus_tag="VFX21_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014652742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5097494.1"
                     /translation="MSRIGNRLDELGIVLPEVFPPAGNYLPCARQGNVLYVGGHGPTD
                     GTRTVIGRVGADLTLEQGREAARLTALSIVATMHAELGDLDRVRRIVKVFGMVNVAPG
                     FNQTPAVIDGCSDVLIDIFGEAGRHTRSAVGLAELPFSIAVEIELIAEIAE"
     gene            3748..>3860
                     /locus_tag="VFX21_15815"
     CDS             3748..>3860
                     /locus_tag="VFX21_15815"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5097495.1"
                     /translation="MRVLPRVIVIGAGFGGLQCARKLRDEPVDVVLVDRQN"
BASE COUNT          632 a         1281 c         1291 g          656 t
ORIGIN      
        1 atgccgcccg gatgcaccgt cgtcgacgac acgccgcacg gatcggtctc gagctcgatg
       61 cgcaacgcgt cggtgaagcc gcgcacaccg aacttcgccg cgttgtacgc cgactgggtc
      121 ggcacgctga ccagaccgaa gatcgacgag atgttgacga catgcccgtc gccggatgac
      181 ttcaggtgcg gcaggaacgc ttgcgtgccg tgcacgacgc cccagaagtt gatgttcatc
      241 aaccactcga agtcgtcgta cgtcatgttg gcgaccgtcg caccgagccc gacgccggcg
      301 ttgttgaaga tgaagttgac gcggccgtgg tcggcaacga cctggtcggc ccacgcctcg
      361 accgcggcgc ggtcggcgac gtcgacgcgc tgcgacgtca ccttgacgcc gaccccctcg
      421 caacgcgtga ccgtcgccgc gagtccggct tcgtcgacgt cacacaacgc gaggtgcgcg
      481 ccccgccgcg ccaggtcttc ggcgagcgct cggccgatgc ccgaccccgc gccggtgatt
      541 gccgccactc gtcccgcgaa ccgcccgcgt ccaccggcgt ccggtccagg gttcgaaaac
      601 tgcatggcgt catcatgcac gctgcgcagc ttcatcgctt cgtagtcgcg catgtaattc
      661 tggtgcatct gccacgggaa cctggtgccg gacttcggga accggtcgat cgcgcgcatg
      721 acgtagcccg acgtgagtcc gatcatcggc ttcgccgcgt ccacgtcacc acgcgacacc
      781 ggcgtcgcct gccgcatgcc ggaggcgcgc agctcgtcga ggatgcggca cacgtagtgc
      841 gccgtgagat cggccttcag cgtccacgac gcgttcgtgt agccgaacgt gttcgcgaag
      901 ttggggacgc gctcgagcat catcccgcga tacaggagcc ggtcggcgat gtcgacgtgt
      961 tcgccgtcga cggtgagctc catgccgccg atgaacaaca ggtcgagacc cgtcgcggtg
     1021 acgatgacgt cggccgacac cgactggccg gacaccagcg cgatgccgtg ttcggtgaag
     1081 gactcgatct cgtcggtgac gaccgtgacc tggccggccg agatcgcctc gaacagatcg
     1141 gcgtcgggga cgacacagaa gcgttcgtcc cacgggtcgt agcgcggcgt gaagtgggtg
     1201 tcgatgtcga agtccgccgg caggcgccgt tcgaggtcac ggcggagcac ccgcttcacc
     1261 aactccggtc gccgttggct caactcgtag aacgcctgtg tccccagcgc gaacgaccac
     1321 cggacggcgg acgcggcgaa ccgttcgggc acatacttct tgatctggtc ggcgcgcgag
     1381 ctttcggacg gcaccgccgc gatatacgtc ggcgaacgct gcaccatcgt cacgtgcgcg
     1441 gcgcgttcgg ccagcgcggg cacgagcgtg accgcagtcg cgccgcttcc gatcacggcg
     1501 acgtgcttac ccgcgaagtc gagatcctcg ggccagaact gcggatgcac gatcggcccc
     1561 tgaaatcggt cgcgcccggc gaatgcgggc tcatacccgt ggtcgtaggc gtaatagccc
     1621 gcgcacgaat gcaagaagct gcacgacaac tgcacgcgct cgttcgtgtc ggtgcgctcg
     1681 gcggtgacgt accaacgggc gtccgctgac gaccagtccg ccgcgatcac gcggtggtgg
     1741 taccggatct tttcgtcgac gccgtactcg gccgcggtgt cacggatgta ctcgaggatc
     1801 gagggaccgt cggcgatcgt cttcgggccc gtccacgggc ggaacgaata gccgagcgtg
     1861 tgcatgtcgg agtcggagcg cacgccggga tagcggaaca ggtcccacgt gccaccgttc
     1921 gtgtgacgcg cctcgagcac cgcgtagctc gcccacgggc acgagatcgt gagatggcac
     1981 gcggcaccga cgccggaaag tccggcgccg atgatcagga cgtctacgtg ctcttgcacc
     2041 ccgcgatgct cgcatacggg atgcaggagt caggtctggc gggatcagca gagaccgtcg
     2101 ggcgtgctcg tgtcgcagac gttgtgctcg aacacgttgc cggttccgga gccgtcccag
     2161 ttgatgtccg gtgtgttgtt cgtgatgatg ttgccacgca ccgtgttgtt cgtcggcggc
     2221 gtgccgggcg cgctcgggtc accaccggcg atcaccgtca caccaccggc gaccggcgag
     2281 tcgccgttgt tgttcgtgat gatgttgccg gtcagcgtca cgtcgtgcgc accgacgagc
     2341 gcggtgccga cgccctggat cgggaagggc gtgtcctcgc tcggcgggca gaaccgattg
     2401 ttctcgaaga tgaagttgcc gaagacgtcc gatgcgccga ccggaccggg cgcgtctccg
     2461 aggaagatca cgccgaggca gttgccgtgc acgcggttgc cgacgatgtg cgcgccttcg
     2521 gcgttgcgga cgaagatgcc gaacagcgcg tcggtcgtgt cgttcccgat gatcttcgca
     2581 tcggcgtcag gagagtcgcc gaagtagaga ccggcttctt ccgcaccgtc gaccttgttg
     2641 gcgatcgcct tggtaccgct cgacatgaag gccgcggtgc cgtattcgcc gttgtcgacc
     2701 aacgtgttgg cgatcatgga gaggtcggtg ccgccgatct gcacgatgcc gatgccttcg
     2761 aacccttcga cgtgcagacc gatgatccgc acaccgtcga tcggatcacc gaccgcaccc
     2821 gtgtcgaagt cgatgtcact cccgatgatg cacacaccgc tcacgaccga cggatcctcg
     2881 gggtcaccgc attcgttctc gaccggtgcc gcgggcggca tcaacgtcgc gccgctgccg
     2941 atgatggtga tgtcgtcggt cgcgatgcgg atgttctcgt gatacgtgcc ggcctgcagg
     3001 atgatcgtgt cgccgggcga cgcgtcgtcg atcgcggcct ggatcgagtc acccggccgc
     3061 acgacatggc ccgccgctgc ggccggcgac gcaaaggcca gacctcccac cagtgcgatc
     3121 gaaccgaaca gggccaagga tcgagcaaac cgggccatga cgcgcccctt ccgtcgggag
     3181 acgcgacgtt gttaccgccg tccgccccct tcgcgtgagg ccctgtttca gccttactca
     3241 gcgatctccg cgatgagttc gatctcgacc gcaatgctga acggaagttc cgccaagccg
     3301 acggcgctgc gggtatgccg cccggcctcg ccgaagatgt caatgagcac gtcggagcag
     3361 ccgtcgatca cggccggggt ctggttgaag cccggtgcga cgttcaccat gccgaagacc
     3421 ttgacgatgc gtcgcactcg atcgagatca ccgagctcgg cgtgcatcgt cgcgacgatc
     3481 gacaacgcgg tcagccgcgc ggcctcgcgg ccctgttcga gcgtgagatc ggcgccgact
     3541 ctgccgatga cggtgcgcgt gccgtcggtg gggccatggc cgccgacgta cagcacgttt
     3601 ccctggcgcg cgcacggcag gtagttcccg gcgggcggga agacctccgg caacacgatg
     3661 ccgagctcgt cgaggcgatt gccgatgcgg gacattcgcg gacgctaccg ccaaaacgga
     3721 ttcgtcgtgc cgctacggtg gacgtcgatg cgtgtcctgc cgcgggtcat cgtcatcggt
     3781 gccggcttcg gtggtttgca gtgcgcgcgc aaactgcggg acgagcccgt cgacgtcgtc
     3841 ctggtcgacc ggcagaacta
//
LOCUS       DASUGA010000803         2096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2003691, whole genome shotgun sequence.
ACCESSION   DASUGA010000803 DASUGA010000000
VERSION     DASUGA010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2003691"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VFX21_15820"
     CDS             <1..191
                     /locus_tag="VFX21_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HEX5097496.1"
                     /translation="SDDSAAGLSIGMAATPEDDDAVVEDRGARVFVESEAVDLLDDKM
                     LDAEIDDGGRVRFMLGPQ"
     gene            285..>2096
                     /locus_tag="VFX21_15825"
     CDS             285..>2096
                     /locus_tag="VFX21_15825"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097497.1"
                     /translation="MLSRRSRLPLSTVVLLALCAAFLASCKVPGAALSGRLLMTGTAQ
                     PAAGARVTVFDAATELAVAETIANADGIYWLNPDRLPAGTYRLRFGASEWWNGATEWS
                     HATDVVVTNGDDTRVVNANVSPGTPKSKWFETGFFIDWAGFGIVNELFNEMGAGGMHA
                     VGPWEPFHDSTDQADFMQRLQGDVGLHAKLARAKATGSPVIVQLEMMPSWLTSAPGNN
                     TLPCADQPLWPTSVTRAPAADKWDDWEALVEETVRYLTVTRGYDNLWFQLWEEPDGPC
                     FWTDTEANYLKLYEATARAVKRANPNARIGGPGSESPTGVIEPSTRPLVQALIEHAGA
                     TGAPLDFVSYHYFGFTPHRAQLVAEDAGDALDRAGFPDAPILISSYNPLESAVSPYWP
                     TPGTPSPTGRTWEYDTEIGAAYVPALSFALAQSGRHGYHTLFQLDDFDGGREYESDAA
                     WGSRTPEERNAIRKAMYQGMRLLSQVPDTLVDATIDDPRAGTDGALGPLGALAGVDGT
                     NVSVLLWSYIAAPEKEANAVVIDHGYPNGLATWNLAKVTAFFGDQAPVSSVTGNPQEQ
                     ATLEAAKRVLFRQRDLVNRPRTVRICGTGLQTAEAVHV"
BASE COUNT          340 a          743 c          716 g          297 t
ORIGIN      
        1 aatccgacga cagcgccgcc ggattgagca tcggcatggc cgcgacgcca gaggacgacg
       61 atgcggtcgt cgaagatcgc ggcgcacggg tcttcgtcga gtccgaagcc gtcgatcttc
      121 tggacgacaa gatgctggat gcggagatcg acgacggcgg ccgcgtcagg ttcatgctcg
      181 gcccccaata gagcagccgg ccgtcatccg acccggagcg ggtccatcac ggcgcgatca
      241 acgcaaggga ggtcgcgccg ttcgacgcga gctaccctca cgacttgctc tctcgacgct
      301 cgcggctacc gctgtcgacg gtcgtcctgc tcgcgctgtg cgccgcgttc ctcgcgagct
      361 gcaaggtgcc gggcgcggct ctgtccggcc gtctgctcat gaccggcacc gcgcagccgg
      421 ccgccggcgc gcgcgtcacc gtgttcgacg ccgcgaccga gctcgcggtc gcggaaacga
      481 tcgcgaacgc ggacgggatc tactggctca acccggaccg gctcccggcg ggtacgtacc
      541 ggctgcggtt cggcgcgtcg gagtggtgga acggtgcgac agagtggtcg cacgcgaccg
      601 acgtcgtggt gaccaacggc gacgacacac gggtggtcaa cgcgaacgtg tcgccgggca
      661 cgccgaagtc gaaatggttc gagaccggct tcttcatcga ctgggccggc ttcggcatcg
      721 tcaacgagct gttcaacgag atgggtgcgg gtgggatgca cgcggtcgga ccgtgggaac
      781 ccttccacga ctcgaccgac caggccgact tcatgcagcg gctccagggt gacgtgggcc
      841 tgcacgcgaa gctcgcgcgg gccaaggcca cgggctcgcc cgtcatcgtg cagctcgaga
      901 tgatgccgtc gtggctcacg tcggcgccgg gtaacaacac gctgccgtgc gcggaccaac
      961 cgctgtggcc gacatcggtc acgcgcgcgc ccgcggccga caagtgggac gactgggaag
     1021 cgctcgtcga ggagaccgtg cgctatctca ccgtgacgcg cggctacgac aacctctggt
     1081 tccagctctg ggaagagccc gacggcccgt gcttctggac cgatacggaa gcgaactacc
     1141 tgaagctcta cgaggcgacc gcccgcgcgg tgaagcgcgc caatccgaac gcgcgcatcg
     1201 gcggccccgg ctccgagagc cccaccggtg tgatcgagcc gtcgacgcgc ccactcgtgc
     1261 aggcgctgat cgagcacgca ggcgccaccg gcgcgccgct cgacttcgtc tcgtaccact
     1321 acttcggctt cacgccgcac cgcgcgcaac tcgtcgccga ggacgccggc gatgcgctcg
     1381 accgcgccgg cttccctgac gcgccgatcc tgatctcgtc ctacaacccg ctcgagagcg
     1441 cggtgtcgcc gtactggccg acgccgggca cgccgtcacc gacgggccgc acgtgggagt
     1501 acgacaccga gatcggcgcc gcgtacgtgc ccgcgttgtc gttcgcgctc gcgcagagcg
     1561 gccggcacgg ctaccacacg ctcttccaac tcgacgactt cgacggtggc cgcgagtacg
     1621 agtccgacgc cgcgtggggc tcgcgcacgc cggaggagcg caatgccatt cgcaaggcga
     1681 tgtaccaggg catgcgcttg ctgagtcagg tgccggacac gctcgtcgac gccaccatcg
     1741 acgacccgcg cgccggcacc gacggcgcgc tcggcccgct cggcgcgctc gccggcgtcg
     1801 acggcacgaa cgtgtcggtg ctcctgtgga gctacatcgc cgcgcccgag aaagaggcga
     1861 acgcggtcgt catcgaccac ggatatccga acggcctggc gacctggaac ctcgcgaagg
     1921 tgaccgcgtt cttcggcgat caggcgccgg tctcgagcgt caccggcaat ccgcaggagc
     1981 aggcgacgct cgaagcggcc aagcgcgtgc tcttccgtca gcgcgacctc gtgaaccggc
     2041 cgcgcacggt acgcatctgc gggaccggcc tgcagactgc ggaagccgtg cacgtc
//
LOCUS       DASUGA010000804         2445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_511904, whole genome shotgun sequence.
ACCESSION   DASUGA010000804 DASUGA010000000
VERSION     DASUGA010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511904"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="VFX21_15830"
     CDS             complement(<1..1281)
                     /locus_tag="VFX21_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097498.1"
                     /translation="MLWLRFRTHWRQRWGSLLALTVLVAIVGSVVLTTLAGARRTESV
                     VERVDEHYQVPDTFALFEDQSTFEQTDAILDSPLVEKGDRIEVIAAFSELGYLPLIAS
                     VDGRFGVDMHFNRLVQGRRPAADAPLEVALPEWAVAATGRGIGDTIPFSSVSKEQGRC
                     LFESGLEASTDPVCDAITAIFDGEPDFSAFAGPAFDLEVVGIVQELDQDDPNAVNSEP
                     IYLSEAFHREYGHLIATSPGLVARFRPGVTDGEFERELSKFVGPEKLEDFTYSSTTFD
                     SLDATASTIASGLLIFAGVAAIAGLVAIAQAVVRQSAAAAADGRVIAALGATRATRAF
                     DPLVPLVPVAVGGALLAVATAWLASPIMPVGSPRDIETSRGFDFDGFVLVAGGAALAL
                     FVLAGGWIAAAWIGRTCRRGSPSRAGAGLPATVPA"
     gene            complement(1469..1957)
                     /locus_tag="VFX21_15835"
     CDS             complement(1469..1957)
                     /locus_tag="VFX21_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX5097499.1"
                     /translation="MPSFDVVSEVDMQEVRNAVDQAHRELSTRFDFKNTDSMIELKDD
                     SIELESASEERLKAAVQVLEEKLVKRKVSLKTLEFGKVEEATKGRARQKAALQSGINQ
                     EKAKAIGKYIKELGLKGVQHQVQGDQLRVIGKKRDDLQTAIAKLKEHDFGIPLQFTNF
                     RD"
     gene            2009..2094
                     /locus_tag="VFX21_15840"
     tRNA            2009..2094
                     /locus_tag="VFX21_15840"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2043..2045,aa:Tyr,seq:gta)
     gene            2161..>2445
                     /locus_tag="VFX21_15845"
     CDS             2161..>2445
                     /locus_tag="VFX21_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097500.1"
                     /translation="MAVHTFDVGAVRLTRVSYAEVDLPPEGVGLTAAAVRSVTWAEPT
                     WANGENPRVAAAAWIIESDGARIVVDPAQAADDILRNDNDAAFHQDAFAAL"
BASE COUNT          398 a          815 c          839 g          393 t
ORIGIN      
        1 ggccggcacc gtggccggaa gcccggcgcc ggcccgcgat ggtgaaccgc gccggcacgt
       61 gcgcccgatc cacgcggccg cgatccagcc gccggccagt acgaagaggg cgagcgccgc
      121 gccgccggcg acgagcacga agccgtcgaa gtcgaagcct cgcgatgtct cgatgtcgcg
      181 cggtgacccc acaggcatga tcggtgacgc gagccacgcc gtcgcgacgg cgagcagcgc
      241 accgccgacc gcgaccggca cgagcggcac gagtggatcg aacgcgcgcg tcgcacgagt
      301 cgcgccgagg gcggcaatca cgcgtccgtc ggccgcggcg gcggccgatt gccgcacgac
      361 ggcttgcgcg atcgcgacca ggccggcaat cgccgccacg cccgcgaaga tcaggagccc
      421 gctcgcaatc gtcgacgcag ttgcgtcgag cgagtcgaag gtggtcgacg aataggtgaa
      481 gtcttcgagc ttttcggggc cgacgaactt ggagagctcg cgttcgaact cgccgtcggt
      541 cacgccgggt cggaaccgtg cgacgaggcc gggactcgtc gcaatcagat gcccgtactc
      601 gcgatggaac gcctcggaga gatagatcgg ttccgagttc actgcgttgg ggtcatcctg
      661 atcgagctct tgcacgatgc cgacgacttc cagatcgaac gcaggaccgg cgaacgcgct
      721 gaagtcgggc tcgccgtcga aaattgcggt gatcgcgtcg cacacgggat cagtcgaggc
      781 ctcgagaccg gactcgaaga ggcagcgtcc ctgctccttg ctcaccgagc tgaatggaat
      841 cgtgtcgccg atgccgcgac cggtcgccgc gaccgcccac tccggaagcg cgacctcgag
      901 cggcgcgtcc gctgccgggc ggcgaccctg cacgagacgg ttgaagtgca tgtcgacgcc
      961 aaaccgtccg tcgacggaag caatgagcgg caagtacccc agctcggaga acgcggcgat
     1021 gacctcgatg cggtcgccct tctcgacgag tgggctatcg aggatcgcgt cagtctgctc
     1081 gaaggtcgac tgatcctcga agagcgcgaa cgtgtcgggc acttgatagt gctcgtcgac
     1141 gcgctcgacg accgactcgg tccggcgcgc gccggcgagc gtcgtcagca cgacggatcc
     1201 gacgatggcc acgagcacgg tgagcgcgag cagcgaaccc cagcgctgcc tccaatgtgt
     1261 ccggaaacgc agccacaaca tcgtcatttc gctctgcgcg cctcacccgt cattgtcgag
     1321 aattccaccg tcgcatacgg cgcgcgccaa gggcgcgggc ttctcggcgg ggcgggatgc
     1381 tggcaccccc aaggcggcgc cggcggctgt cattttggcg tgagcctcct ccgaccgacg
     1441 aagcgggcga aggccagcgc ggcgggtgct agtcgcggaa gttcgtgaac tggagcggga
     1501 tgccgaagtc gtgttccttc agcttcgcga tcgcggtttg caggtcgtcg cgcttcttgc
     1561 cgatgacgcg gagctggtcg ccctgcactt ggtgctggac gcccttgagg ccgagctctt
     1621 tgatgtactt gccgatcgcc ttggccttct cttgattgat gcccgactgg agcgcggcct
     1681 tctgacgagc gcggcccttg gtggcctctt cgaccttgcc gaactcgagc gtcttgagcg
     1741 agaccttgcg cttgacgagc ttctcctcga gcacttgcac cgcggctttg aggcgctcct
     1801 cggacgcgga ctcgagctcg atcgagtcgt ccttcaactc aatcatgctg tcggtgttct
     1861 tgaagtcgaa gcgggtcgac agctcgcggt gcgcttggtc caccgcgttg cggacttcct
     1921 gcatgtccac ttcggagacg acgtcgaagc tcggcacggg ggctccttca ctgggcgtcc
     1981 ggcggaggcg gcccgctaga ttagacccgg gtcggtaccc gagtggccaa aggggacggg
     2041 ctgtaaaccc gttgcgttac cgctacgttg gttcgaatcc aacccggccc accaggcccg
     2101 cctttcgagg cgggcctttt tcgttcgccc gcaggcgcgg gggggcgcga gcatgcaccg
     2161 atggcggtcc acacgttcga cgtcggcgcc gtacggctca cgcgtgtgag ctatgcggag
     2221 gtcgacctgc cgcccgaggg cgtcggtttg accgcggccg cggtgcggag cgtcacctgg
     2281 gccgagccga cgtgggcgaa cggcgagaac ccacgagtgg ccgcggccgc gtggatcatc
     2341 gagagcgacg gcgcccgcat cgtcgtcgat ccggcccagg ccgccgacga catcttgcgc
     2401 aacgacaacg acgccgcgtt ccaccaagac gcgttcgcgg ccctg
//
LOCUS       DASUGA010000805         2888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_667181, whole genome shotgun sequence.
ACCESSION   DASUGA010000805 DASUGA010000000
VERSION     DASUGA010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667181"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1467)
                     /locus_tag="VFX21_15850"
     CDS             complement(<1..1467)
                     /locus_tag="VFX21_15850"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5097501.1"
                     /translation="MDATETLDALAGEPELAGRLVYRDVLPARDARFGSLSEPLHRDV
                     NARLRARGVEQLYSHQAAAIDQLRAGTNTVLATSTASGKSLCYQVPIVESVVTGRRDT
                     ALLVFPTKALAQDQLRSLRSWLVSGLRAVTYDGDTDRDDRAWARKNANVILTNPEMLH
                     VGILPGHAQWGTFLKRLRFVVVDELHTLRGIFGSQVAHVLRRLRRLCEHYGASPTFCF
                     ASATIGNPAELASDLLGAPVTAIDDDGSPRAERVLACWQRPLVDAQSGARSSANAETA
                     DLLARFVAAGHPSLAFTRSRRGAELVAQYARDRLQDDEDYRGQRVAAYRAGYLPEERR
                     QIEKDLSSGRLMGLAATTALELGIDVGGLDAVVLNGFPGTLASMWQQAGRAGRNGRRS
                     AAVLVAGDDQLDQWYAAHPKELTHRTPERAVVNPQNPFVLRAQVACAAHELPLTNDDE
                     RFFGDGLDDTVRELVHADLLRPRGGKMYWCGERSPAPGV"
     gene            complement(1545..2462)
                     /locus_tag="VFX21_15855"
     CDS             complement(1545..2462)
                     /locus_tag="VFX21_15855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX5097502.1"
                     /translation="MKRLFRKKIVKRVALVTTSAFLVMTLACVGLMGAWLAGYKVPVV
                     SGATWMTVEKVGSADAAGSPDGVFFIALIGSDARPGVGGARGDALHLVGVNPATNTAT
                     MLNVPRDTCWAGGKINRAHAGDGGPRGQADALGELTGTNVSYAVSVDFAGFQGIVDGV
                     GGVQIDVPTNMDDEFSGAVFNAGPNHLDGFHALAYSRDRHDFPRGDITRSDNQGLLIL
                     AGLAQMQNEAKGPAGDFKLAALLGRHAQLDGVGLTDLYRLGRVAHKLDLTKVRNVTIP
                     VGGGQCLPLVGDAGGLFADFRDDATLQTH"
     gene            complement(2459..>2888)
                     /locus_tag="VFX21_15860"
     CDS             complement(2459..>2888)
                     /locus_tag="VFX21_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097503.1"
                     /translation="AAQAYVDAATLAPLTTGKVGDAYDGLFDTSVSTNATGPDRAALT
                     DDGIAEATTSPEITATPVRIDALADKDGKVLLLGATFDLDVRAESAAGPVSVHRTNEL
                     TYAPVDGTWKITAYRVDVQRDTPEGATSTTADTTTETTTK"
BASE COUNT          412 a         1023 c         1018 g          435 t
ORIGIN      
        1 gacgccgggt gccggggacc gctcgccgca ccagtacatc ttcccgccgc gcggccgcag
       61 gagatcggcg tgcacgagct cgcgcaccgt gtcgtcgaga ccatcgccga agaaccgctc
      121 gtcgtcgttc gtgagcggca gttcgtgcgc cgcgcacgcg acctgcgcgc gcaacacgaa
      181 cgggttctgc gggttcacga ccgcgcgctc gggcgtgcga tgcgtcagct ccttcgggtg
      241 cgcggcgtac cactggtcga gctggtcgtc gccggcgacg agcaccgcgg ccgagcgccg
      301 gccgttgcgg cccgcgcggc cggcttgttg ccacatcgac gcgagggtgc cggggaagcc
      361 gttcaggacg acggcgtcga gaccaccgac gtcgatcccg agctcgagcg cggttgtcgc
      421 cgccaatccc atgagccgtc cactcgagag atccttctcg atctgccgcc gctcttcggg
      481 gagatacccg gcgcgatacg cggcgacgcg ctggccgcgg tagtcctcgt cgtcctggag
      541 gcggtcacgc gcgtactgcg cgacgagctc cgcgccccgc cggctgcgcg tgaacgcgag
      601 actcggatga cccgccgcga cgaaccgggc caacagatcg gccgtctccg cgttcgccga
      661 cgaacgggca cccgactgcg cgtcgacgag gggccgctgc cagcacgcga gcacgcgttc
      721 ggcgcgcggt gaaccgtcgt cgtcgatcgc ggtgaccggt gcaccgagca gatcgctcgc
      781 cagctccgcg gggttgccga tcgtcgcgct cgcgaagcag aacgtcggcg acgcgccgta
      841 gtgctcgcac agtcggcgca ggcggcgcag cacgtgcgcg acctggcttc cgaagatgcc
      901 acgcaacgtg tgcagctcgt cgacgacgac gaaccgcagc cgcttgagga aggtgcccca
      961 ctgcgcgtgc ccgggcagga tgccgacgtg caacatctcc ggattcgtga ggatgacatt
     1021 cgcgttcttg cgcgcccacg cccggtcgtc gcggtcggtg tcgccgtcgt aggtcaccgc
     1081 ccgcaggccg gacacgagcc acgaacgcaa cgaccgcagc tgatcctgcg ccagcgcctt
     1141 cgtcgggaag accaataacg cggtgtcgcg ccgccccgtc acgaccgact cgacgatggg
     1201 cacctggtag cagagcgact tgccgctcgc cgtcgaggtg gcgagcacgg tgttcgtccc
     1261 ggcgcgcagc tggtcgatgg ccgcggcctg gtgcgagtac agctgctcga cgccccgcgc
     1321 ccgcagccgc gcgttcacgt cgcggtgcaa cggctccgac agcgatccga accgcgcgtc
     1381 gcgagccggc aacacatcgc gatacacgag ccgcccggcg agctccggct ccccggccaa
     1441 cgcatccagc gtctcggtcg catccacggc gcaaacgcta ccgagaggcg ccgacatcgc
     1501 cgcttctccg gaatctccga cctcaacgcg tgctcgcggg cgcgttagtg cgtttggagg
     1561 gtggcgtcgt cgcggaagtc ggcgaagagg ccgccggcgt cgccgacgag cgggaggcac
     1621 tgcccgccac cgacggggat cgtcacgttg cggaccttcg tcaggtcgag cttgtgcgcg
     1681 acgcggccga gccggtacag gtcggtgagc cccacgccgt ccagctgcgc gtggcggccg
     1741 agcagcgcgg cgagcttgaa gtcgccggcg ggccccttcg cctcgttctg catctgcgcg
     1801 aggccggcga ggatcaacag gccctggttg tcgctgcgtg tgatgtcgcc gcgtgggaag
     1861 tcgtgacggt cgcgtgagta cgcgagggcg tggaagccgt cgaggtggtt cgggcccgcg
     1921 ttgaacacgg cgccggagaa ctcgtcatcc atgttcgtcg ggacgtcgat ctgcacgcca
     1981 cccacaccgt cgacgatgcc ctggaagccg gcgaagtcaa cggacaccgc gtacgacacg
     2041 ttcgtgccgg tcagttcgcc gagcgcgtcg gcctgtccgc gcgggccgcc gtcaccggcg
     2101 tgcgcccggt tgatcttccc gccggcccag cacgtgtcac gcgggacgtt caacatggtc
     2161 gcggtgttcg tggcggggtt cacgccgacg aggtgcagcg cgtcaccgcg ggcgccgccc
     2221 acaccggggc gggcgtcgct gccgatgagc gcgatgaaga acacgccgtc gggtgagccg
     2281 gccgcgtcgg ccgagccgac cttctcgacc gtcatccacg tggcgccgga cacgacggga
     2341 accttgtaac cggcgagcca ggcgcccatc agcccgacgc acgcgagcgt catcacgagg
     2401 aacgcgctgg tggtcaccag cgcgacgcgc ttgacgatct tcttgcggaa gaggcgcttc
     2461 acttcgtcgt cgtctccgtc gtcgtgtcgg cggtcgtcga cgtcgcaccc tcaggcgtgt
     2521 cgcgctggac gtcgacgcgg tatgcggtga tcttccaggt gccgtcgacc ggtgcgtagg
     2581 tcagttcgtt ggtgcggtgc accgacaccg gtccggcggc gctctcggcg cgcacgtcga
     2641 ggtcgaaggt cgcgccgagc aacagcacct tgccgtcctt gtcggcgagc gcgtcgatgc
     2701 gcaccggcgt tgccgtgatt tcggggctcg tcgtcgcctc tgcaatgccg tcgtcggtca
     2761 gcgccgcacg gtcggggccg gtcgcgttcg tgctcaccga cgtgtcgaac aagccgtcgt
     2821 aggcgtcacc aaccttcccg gtcgtcagcg gcgcgagcgt tgccgcatcg acatacgcct
     2881 gcgccgcg
//
LOCUS       DASUGA010000806         2469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_93916, whole genome shotgun sequence.
ACCESSION   DASUGA010000806 DASUGA010000000
VERSION     DASUGA010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93916"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..412
                     /locus_tag="VFX21_15865"
     CDS             62..412
                     /locus_tag="VFX21_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX5097504.1"
                     /translation="MPGGVTIDDARAIATTLPRSYEAVVRDSVRFRVGRLVYAAFYDD
                     DTVMGFGFPKEEREALVASEPEKFLMPRASDMRYRWVCVRLDAIDVDELRELLVDAWR
                     MCVPKSVAAAYDDC"
     gene            complement(428..1054)
                     /locus_tag="VFX21_15870"
     CDS             complement(428..1054)
                     /locus_tag="VFX21_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097505.1"
                     /translation="MTEAFRSDDGTAVDSAIARDEWTRAARRYLVTVARRYGALTTPN
                     DVAEEAQAGSGIRTKEEPDTWVGDVIDEVGVECATRNEPLLSAFCIGPEGRIGDRYRQ
                     VLVMLERPLPADLEMDAAKQRLEAHEYHGATMPVDPRPALPPALAKARASAPKQRSSS
                     TRAPSTPRVRKPAAPRKPKKPEPPPRAVCPTCFLQLPATGNCDNCNPD"
     gene            complement(1085..>2469)
                     /locus_tag="VFX21_15875"
     CDS             complement(1085..>2469)
                     /locus_tag="VFX21_15875"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5097506.1"
                     /translation="GNPVARTPHLDALAARGTRFAQAYSQHSACAQSRISMQTGWYPH
                     VAGHRTLDHLLQPWEPNVFKTLRAAGYYVAWAGIRGDTFAPGVTAESTDFFGYTVRPS
                     LDAIAASHREAFPPGHRLRHAHYVGCLEDAGLDFDEAAVQTAIQLLGDGLPEPFVLVL
                     TLFAPHPPFAVAEPWFSLHARSDMPAPVAGDGKAAFVAALRERGELDRLERADWAEIS
                     ATYYGMVSRVDDQLGRVLEALDRAGAAERTVTCSFTDHGEYLGDYGIVEKWPSGLDDC
                     LLRNPLVIAGPNVGEGVTSAALVEMIDLVPTWCELAETDVGHPQFGQSLVSLLTGEAA
                     DHRAAVFSEGGFRLDEESQNELRADYPYDLKTGLLHDEPHLVGRAVAIRTNAFAYVYR
                     REEADELYDRVADPAETRNLAGDAAHAATVAELRDQVLRWFVETSDVIPQARDPRMEP
                     ALVQQFLPGA"
BASE COUNT          392 a          822 c          860 g          395 t
ORIGIN      
        1 ggcagaacat cagcttccca agctgagaac gcgggttcga ttcccgtcgc ccgctctgga
       61 tatgccagga ggggtcacga tcgacgacgc ccgggcgatc gccacgacgc tgccacgttc
      121 gtacgaggcc gtcgtgcgcg actcggtgcg cttccgcgtc ggccgcttgg tctacgcggc
      181 cttttacgac gacgacacgg tcatgggttt cggtttcccc aaggaggaac gcgaggcgct
      241 cgtggcttcg gagcccgaaa agttcttgat gccgcgcgcg tcggacatgc ggtaccggtg
      301 ggtgtgcgtc cggctcgacg cgatcgacgt cgacgagctg cgagagctgc tcgtcgacgc
      361 gtggcgcatg tgcgtgccga agagcgtggc cgccgcgtat gacgattgct gaacccgagt
      421 gtgcaaatca gtcggggttg cagttgtcgc agttgcccgt cgcggggagc tgcaagaagc
      481 aggtcgggca caccgcgcgg ggcggcggct cgggcttctt cggcttccgg ggtgcagccg
      541 gcttgcgcac ccgcggcgtc gacggcgcac gcgtcgacga actccgttgc ttcggcgccg
      601 acgcgcgcgc cttcgcaagc gccggcggca gcgccggacg cgggtccacc ggcatcgtcg
      661 cgccgtggta ttcgtgcgcc tcgagccgtt gcttggcggc gtccatctcg agatcggccg
      721 ggagcggacg ctcgagcatc acaagcactt gccggtagcg atcgccgatg cgtccctccg
      781 gtccgatgca gaacgccgac aacaacggct cgttgcgggt cgcacactcg acgccgacct
      841 cgtcgatgac gtcgcccacc cacgtgtccg gttcttcctt cgtccgaatg cccgatcccg
      901 cctgcgcttc ttcggccacg tcattcggcg tggtcaatgc gccgtagcgg cgcgcgaccg
      961 tgacgaggta ccgccgcgcc gcgcgcgtcc actcgtcgcg cgcaatggcc gaatcaaccg
     1021 ccgtgccgtc gtcgctccgg aatgcctctg tcatcgaact gcaccgtagg ccacggaggc
     1081 ggggtcacgc gccgggcaga aattgctgaa cgagcgcggg ctccatccgc gggtcgcgcg
     1141 cttgcggaat gacgtcgctc gtctcgacaa accagcgcag gacctggtcg cgaagctcgg
     1201 cgacggtcgc agcgtgggcg gcgtcgccgg cgaggttgcg ggtctcggcc gggtcagcaa
     1261 cccggtcgta cagctcgtcc gcttcctcgc ggcgatacac gtacgcaaat gcgttcgtgc
     1321 ggatcgcaac cgcccgcccg acgagatgcg gttcgtcgtg caacagcccg gtcttcaggt
     1381 cgtaggggta gtcggcgcgc aactcgttct gggactcctc gtcgagccgg aacccgccct
     1441 ccgagaagac ggcggcgcga tggtctgctg cctcgccggt caggagcgat accaacgatt
     1501 gcccgaactg cggatgccca acgtcggttt ccgccagttc gcaccacgtc ggaacgagat
     1561 cgatcatctc gaccaaggcg gcggacgtga cgccttcgcc gacgttcgga cccgcgatga
     1621 cgagcgggtt gcgaagcagg caatcgtcga ggcccgacgg ccacttctcg acgatgccgt
     1681 agtcaccgag gtattcgccg tgatcggtga aggaacaggt gacggtgcgt tccgcggcac
     1741 ccgcgcggtc cagggcctcg aggacgcgcc cgagctgatc gtcgacgcgc gacaccatgc
     1801 cgtagtaggt cgcgctgatc tcggcccagt cggcccgttc aaggcggtcg agttcgccgc
     1861 gttcgcgcag cgcggccacg aacgcggcct tgccgtcgcc ggcgaccggc gcgggcatgt
     1921 cgcttcgcgc gtgcagcgag aaccacggtt cggcaaccgc gaacggcggg tgcggcgcga
     1981 acagcgtcag cacgagaacg aaaggttccg gcaagccgtc accgagcagc tggatcgcgg
     2041 tctgcactgc ggcttcgtcg aagtcgagcc cggcgtcttc gagacaacct acgtagtggg
     2101 cgtgccgcag gcgatgcccg ggcggaaacg cttcccgatg cgacgccgcg atcgcgtcga
     2161 gcgacggccg cacggtgtaa ccgaaaaaat cggtggactc ggcggtgaca ccgggcgcaa
     2221 aggtatcgcc gcgaatgccg gcccaggcga cgtagtaacc ggcggcgcgc aacgtcttga
     2281 agacgttggg ttcccacggt tgcagaagat gatcgagcgt ccggtgaccg gccacgtgcg
     2341 ggtaccaacc ggtctgcatc gagatgcggc tctgcgcgca cgcgctgtgc tgcgagtagg
     2401 cctgcgcaaa acgggttccg cgcgcggcca gcgcatcgag atgcggggtc cgcgcaaccg
     2461 ggttgccga
//
LOCUS       DASUGA010000807         2595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_372849, whole genome shotgun sequence.
ACCESSION   DASUGA010000807 DASUGA010000000
VERSION     DASUGA010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372849"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VFX21_15880"
     CDS             <1..430
                     /locus_tag="VFX21_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5097507.1"
                     /translation="APISEERVSEASAVNSAAGSFGLSFGLAFAGAVMLAALSIAFTA
                     QAQSSDVLPAAAQDQVAQALEDDAEVMSNTNLEEQLAGQPQDVQDEIIRINTDARPIA
                     LQVALLIPILAGLIGLFNAFRMMRIPEPEPSGAAEGMAFG"
     gene            506..1342
                     /locus_tag="VFX21_15885"
     CDS             506..1342
                     /locus_tag="VFX21_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019925858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX5097508.1"
                     /translation="MTTIRAALVQTTWTGDKESMIKAHEEYARQAAEQGARVICFQEL
                     FYGPYFCQVQDAAYYEYAESIPGPTTERFQALAAELGLVMVLPMYEEEQPGVLYNTAA
                     VVDADGKYLGKYRKTHIPQVKGFWEKFYFRPGNLGFPVFDTAVGRVGVYICYDRHFPE
                     GWRALGLNGAHIVFNPSATSRGLSAYLWKLEQPASAVANEYFIGAINRVGIEDLGDND
                     FYGTSYFVDPEGKFVGDVGDGNEAELIVRDLDMDLLAEVRNRWAFYRDRRPDAYEGLV
                     QP"
     gene            1339..>2595
                     /locus_tag="VFX21_15890"
     CDS             1339..>2595
                     /locus_tag="VFX21_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020632850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5097509.1"
                     /translation="MSDLRARHRAVAPSWLTLYYDEPIELVRGEGRYVWDRDGTRYLD
                     FFGGILTTISGHSVPEVVDALREQVGRLLHTSTLYLSEPMVELAERLSALSGIPDAKV
                     FVTTSGSEANEAALLMATSYRRSNQILAMRNSYHGRTFGAIAVTANRSWSPTSLSGLQ
                     VSFVHGGYRLRSPYRALDDAAYTDACVQDLRDVVDMCTSGDVACMIAEPIQGVGGFAT
                     PPDGFFGAMGKVLDEYGILFVADEVQTGFGRTGDHFWGYQAHDIVPDLITVAKGIGNG
                     VTLGAVIGRGDVVDSVPANSISTFGGNPLSCAAAIANLDYLLAHDLQTNARVQGQALA
                     AVLRELASTHSYIADVRGRGLMLAIECCVPNTEGRPGGPEPGPAAAAALMEATRRLGL
                     LVGKGGLYGNVLRIAPPLSVTADEIAD"
BASE COUNT          411 a          881 c          892 g          411 t
ORIGIN      
        1 ggcgccgatc tccgaggagc gggtcagcga ggcgtcggcc gtgaactccg cggccggctc
       61 gttcggactg tcgttcggcc tggccttcgc cggtgcggtc atgctcgcgg cgctgtcgat
      121 cgccttcacc gcccaggcgc agtccagcga cgtgctgcct gccgccgcgc aggaccaggt
      181 cgcccaggcg ctcgaggacg acgccgaggt catgagcaac accaacctgg aggagcagct
      241 ggccgggcag ccgcaggacg tccaggacga gatcatccgc atcaacaccg acgcgcgacc
      301 gatcgcgctg caggtggcat tgctgatccc gatcctggcc ggcctcatcg gcctcttcaa
      361 cgcgttccgg atgatgcgca tccccgagcc cgagccgtcg ggcgcggccg agggcatggc
      421 cttcggctga ttcgtcctcg gccgatcgcg acgggctggg cgtcgcgtat cgttggccca
      481 tcgcacctcg agctcgggag tggccatgac gacgattcgg gccgcgctgg tccagacgac
      541 gtggaccggc gacaaggaat cgatgatcaa ggcccacgag gagtacgccc gccaggccgc
      601 cgagcaaggc gcacgggtca tctgcttcca ggagctgttc tacggcccgt acttctgcca
      661 ggtgcaagac gccgcgtact acgagtacgc ggagtcgatc cccggcccga ccaccgaacg
      721 cttccaggcg ctggccgccg agctcggcct ggtgatggtg ctgcccatgt acgaggaaga
      781 gcagcccggc gtgctctaca acacggccgc ggtggtcgac gcggacggca agtacctcgg
      841 caagtaccgc aagacgcaca tcccgcaggt gaagggcttc tgggagaagt tctacttccg
      901 gcccggcaac ctcgggttcc cggtgttcga caccgccgtc ggtcgggtcg gcgtgtacat
      961 ctgctacgac cgccatttcc ccgaagggtg gcgcgcgctc gggctcaacg gcgcgcacat
     1021 cgtcttcaac ccgtcggcaa cgtcgcgcgg attgtcggcg tacctctgga agctggagca
     1081 gcccgcgtct gcggtcgcca acgagtactt catcggcgcg atcaatcggg tcggcatcga
     1141 agacctcggc gacaacgact tctacgggac gagctacttc gtcgatcccg agggcaagtt
     1201 cgtcggcgac gtcggtgacg ggaacgaggc cgagttgatc gtgcgcgatc tcgacatgga
     1261 cctcctcgcc gaggtgcgca accgctgggc gttctaccga gatcgccggc cggacgccta
     1321 cgaggggctc gtgcagcctt gagtgacctg cgcgcgcgtc accgcgcagt cgcgccgagc
     1381 tggctgacgc tctactacga cgaaccgatc gagctcgtgc gcggcgaggg ccgctacgtc
     1441 tgggaccgcg acggcacgcg gtatctcgac ttcttcggcg gcattctcac gacgatcagc
     1501 ggccacagcg tcccggaagt cgtcgacgcg ttgcgcgagc aggtgggcag gttgctgcac
     1561 acgtcgacgc tgtacttgag cgaaccgatg gtggagctcg cggaacggct gtcggctctg
     1621 tccggcatcc ccgacgcgaa ggtgttcgtc acgacgtcgg gatcggaggc gaatgaagcg
     1681 gcgctgctca tggcgacctc gtatcggcgc agcaaccaga tcctcgcgat gcgcaacagc
     1741 taccacgggc gcaccttcgg cgcgatcgcg gtcaccgcga accggtcgtg gtcaccgacg
     1801 agcctctccg ggctgcaggt gtcgttcgtg cacggcggct accggctgcg cagcccgtac
     1861 cgcgccctcg acgatgccgc gtacaccgat gcgtgtgtgc aagacctgcg cgacgtcgtc
     1921 gacatgtgta cgagcggtga cgtcgcgtgc atgatcgcgg agccgatcca aggcgtgggc
     1981 ggcttcgcca cgccgcccga cggattcttc ggtgcgatgg ggaaggtgct cgacgagtac
     2041 ggcatcctgt tcgtcgccga cgaagtgcag accgggttcg gtcgtacggg cgatcacttc
     2101 tggggctacc aggcccacga catcgtgccc gacctcatta ccgtcgcgaa gggcatcggc
     2161 aacggcgtga cgctcggtgc ggtgatcggc cgcggtgacg tcgtcgacag cgtgcccgcc
     2221 aacagcatct cgaccttcgg cggcaaccca ctctcgtgcg cggctgcgat cgcgaacctc
     2281 gactacctcc ttgcgcacga cctgcagacg aacgcgcgcg tgcagggcca ggcgttggcg
     2341 gcggtgctgc gcgagctcgc gtcgacacac tcgtacattg ccgacgtgcg cgggcggggg
     2401 ctgatgctcg caatcgagtg ctgcgtgccc aacaccgagg gccgcccggg cggtcccgaa
     2461 cccgggcccg cggccgcggc cgcgctcatg gaggcgaccc gccggctcgg cctgttggtc
     2521 ggcaagggcg ggctctacgg caacgtgctg cgcatcgcgc cgccgttgag cgtgaccgcc
     2581 gacgagatcg cggac
//
LOCUS       DASUGA010000808         2561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1412496, whole genome shotgun sequence.
ACCESSION   DASUGA010000808 DASUGA010000000
VERSION     DASUGA010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412496"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="VFX21_15895"
     CDS             <1..1149
                     /locus_tag="VFX21_15895"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX5097510.1"
                     /translation="QHANFGRVWMSSWAFGIEWKDTGLGNYTNRLDRAWQLDHVLDEA
                     ARDDIYVMLTLVNHGQFSQFFNSEWADNPYNAANGGPLHAPADFFTDATARRLFEQRL
                     RYIVARWGSSTHVMAWELWNEVDLTFASSPAITAWHSEMAAELRALDPYDHLVTTSHA
                     LFANDPAVWKLDGLDFTQLHFYADSFPSFANLPRTVTSFTRDRLTQTGKPVLFGELGV
                     DSRGPVETVQNDPGGIGVHDGLWGGTVSGGIGTAMSWWWDSVIAADPQRYYPMFGSVA
                     RFVDGVAWDRETFTPATATVTGGNRTVAPYGLRGKRSLLLWLKDDAFQWNTPQDADVT
                     GATLELPGRWCGDWYDTWNGAWLTRVKFKDRVSVPTFRRDIALRARQC"
     gene            complement(1140..1667)
                     /locus_tag="VFX21_15900"
     CDS             complement(1140..1667)
                     /locus_tag="VFX21_15900"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5097511.1"
                     /translation="MNSTILTDSQRLEVRLPVEGDRARFVELFRDDDFMVFSGEVHDV
                     ASANARFDEMLRRAAEMPFAKQPVVERASDEIVGYAGVNWCEFEGARWLEFGWRLVRE
                     ARGRGYATEAARAVLDLAAATFDGEFLCIIDPGNAPSRRVADKLGFVHWKRATWEGDT
                     VDLLRIRPGARRDQH"
     gene            complement(1642..>2561)
                     /locus_tag="VFX21_15905"
     CDS             complement(1642..>2561)
                     /locus_tag="VFX21_15905"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX5097512.1"
                     /translation="NEFEFTFNWAYASRTSTAYFSSARLPERARGLDRRLPTLGTGDY
                     EWRGYLREQQHPHSVQGPGGLLLNWNNQSAPGFMHGDDTPFGSVHRVELFDRFPRDVA
                     LTDDVSIMNRAATEDGRSLVWPVVSAVLRTGPAPNAVDASALAVLDDWVRRDAPRLDA
                     NLDGLNDDAGAPIMDAVWRPIAEAVMRPVLQDELGAVDSLRGLGGLAGQSYVDKDLRT
                     LLDPHHVKGRFNLRYCGNGSLSACRASLWAAFDQAIVPLVQSQGSDPGTWRGPASRTG
                     FTPGLIPATIRSTNRPTFQQVIEFDNPHR"
BASE COUNT          425 a          875 c          852 g          409 t
ORIGIN      
        1 cagcacgcga acttcggacg cgtctggatg tcgtcgtggg cgttcggcat cgagtggaag
       61 gacaccggtc tcggcaacta cacgaaccgg ctcgaccgcg cgtggcagct cgaccacgtg
      121 ctcgacgaag cggcgcgcga cgacatctac gtgatgctga ccctcgtgaa ccacggccag
      181 ttctcgcagt tcttcaacag tgagtgggcg gacaatccct acaacgcggc caacggcggt
      241 ccgctgcacg cgccggccga cttcttcacc gacgcgaccg ctcgccgtct gttcgaacag
      301 cggctccggt acatcgtggc tcgctggggt tcgtcgaccc acgtcatggc atgggagttg
      361 tggaacgagg tcgacctgac gttcgcgtcg tcgcccgcca tcacggcgtg gcactcggag
      421 atggccgccg aactgcgcgc gctcgacccg tacgaccatc tcgtcacgac cagccacgcg
      481 ttgttcgcca acgatccggc cgtctggaag ctcgacgggc tcgacttcac gcagttgcac
      541 ttctacgcgg actcgttccc atcgttcgcg aacctgccgc gcaccgtcac gtcgttcacg
      601 cgcgatcggc tcacacagac gggcaaaccc gtgttgttcg gtgagctcgg cgtcgactca
      661 cgcggcccgg tagagaccgt ccagaacgac ccgggaggca tcggcgtgca cgacgggttg
      721 tggggcggca cggtctcagg tggcatcggt accgcgatga gttggtggtg ggacagcgtg
      781 atcgccgccg atccgcagcg ctactacccg atgttcggtt ccgttgcgcg cttcgtcgac
      841 ggggtcgcct gggatcgcga gacgttcacg ccggcaacgg caaccgtgac cggtggcaac
      901 cgcaccgtcg ctccctatgg ccttcgtggc aaacgctcgc tgttgctgtg gctcaaggac
      961 gacgcgttcc agtggaacac gccgcaggac gccgacgtca ccggcgcgac tttggagctg
     1021 cccggtcggt ggtgcggcga ttggtacgac acgtggaacg gtgcgtggct gacgcgtgtg
     1081 aagttcaagg accgcgtctc cgtgccgacg ttccgccgcg acatcgcgct gcgcgcgcgt
     1141 cagtgctgat cgcgccgcgc accgggccga atgcggagga gatcgacggt gtcaccttcc
     1201 cacgtcgctc gcttccaatg cacgaagccc aacttgtctg cgacccggcg tgacggtgcg
     1261 ttgccggggt cgatgatgca caggaactca ccgtcgaagg tcgcggccgc gaggtcgagc
     1321 acggcgcgcg ccgcctccgt cgcgtagcca cgaccgcgcg cctcccgcac gagccgccag
     1381 ccgaactcga gccaacgggc gccctcgaac tcgcaccagt tcacaccggc gtagccaacg
     1441 atctcgtcgg atgcgcgttc gacgacgggc tgcttcgcga acggcatctc ggccgccctc
     1501 cgcagcatct cgtcgaagcg agcattcgcc gacgcgacgt catggacctc gcccgagaac
     1561 accatgaagt cgtcgtcgcg gaacagctcg acgaaccgcg cgcgatcgcc ctcgacggga
     1621 aggcggactt cgagacgctg actatcggtg aggattgtcg aattcaatca cctgctggaa
     1681 cgtcggccgg ttcgtagatc ggatcgtcgc cgggatcagg cccggcgtga agcccgtgcg
     1741 cgacgcgggg ccgcgccacg ttcccggatc tgaaccctgg ctctgcacga gcggaacgat
     1801 cgcttggtcg aatgcggccc acagcgacgc gcgacatgcg ctcaacgacc cgttgccgca
     1861 gtagcgaagg ttgaaccggc ccttcacgtg gtgtggatcg agcaacgtgc gcaggtcctt
     1921 gtcgacatac gactggcccg ccagcccgcc gaggccgcgc agcgagtcga ccgcaccgag
     1981 ctcgtcctgg agcacgggcc gcatcacggc ttccgcgatc ggccgccaca ccgcgtccat
     2041 gatcggcgcg ccggcgtcgt cgttcagacc gtcgaggttc gcatcgagcc ggggcgcgtc
     2101 gcggcgaacc cagtcatcga gcaccgcaag cgccgatgca tcgacggcat tgggcgccgg
     2161 cccggtgcgc aagaccgcgc tgacgacggg ccagaccagc gaccgaccgt cctccgtcgc
     2221 ggcgcggttc atgatgctca cgtcgtcggt gagggcgacg tcgcgcggga agcgatcgaa
     2281 gagctccact cggtgcacgg aaccgaacgg cgtgtcgtca ccgtgcatga agcccggcgc
     2341 cgattggttg ttccagttga gcagcaatcc gccgggaccc tgcaccgagt ggggatgctg
     2401 ctgctcgcgc agatagccgc gccattcgta gtcgcccgtg ccgagggtcg gcagccgccg
     2461 gtcgaggccg cgagctcgct cgggcagtcg cgccgacgag aagtacgcgg tcgacgtgcg
     2521 cgacgcgtac gcccagttga aggtgaactc gaactcgttc g
//
LOCUS       DASUGA010000809         4358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_373198, whole genome shotgun sequence.
ACCESSION   DASUGA010000809 DASUGA010000000
VERSION     DASUGA010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373198"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..767)
                     /locus_tag="VFX21_15910"
     CDS             complement(87..767)
                     /locus_tag="VFX21_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEX5097513.1"
                     /translation="MSEVEAGPEVDVVPRPPAPEPHPVLQWVQQTRRNPRFALGAVAV
                     VALSAGVALWRAGQDETPARPPAEANAAFVTTTTAVAAPAVVHVVGAVRAPGVVELGA
                     HARVVDAIGAAGGATDDADLQRLNLAALVTDGQRIAVPRVGEPDPAPALGDTVAADGG
                     PTGPIDVNTATEAQLETLPGIGPSLAAAIVAEREKGPFKSVDDLGRVRGIGDARLDQL
                     RELVTVSG"
     gene            complement(849..3812)
                     /locus_tag="VFX21_15915"
     CDS             complement(849..3812)
                     /locus_tag="VFX21_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX5097514.1"
                     /translation="MSPAEGQATDDTPKFRYDATLANEIEQRWQEYWESERVFWTPNR
                     TGLLAEDPRGIADRPSLFVLDMFPYPSGKGLHVGHPLGFIATDVYARFQRMNGFNVLH
                     AMGYDAFGLPAEQYAVQTGTHPRVTTDQNIATMKAQLRALGIGHDPRRGPATTDPAYY
                     RWTQWIFLQIFNSWYDTAQDKARPIAELVDEFRAGTRATPTGGAFDALDESAQRELVD
                     SFRLAYVAEVPVNWCPGLGTVLANEEVTADGRSERGNFPVYRRPLKQWMMRITAYADR
                     LLDDLEQLDWTDSIKLMQRNWIGRSTGATVRFPVVGPENHEIEVFTTRPDTLFGATYM
                     VLAPEHPLVEHIAGNEWPGDDIFVDVGSNVLDDWKGLFGAVGTPADAIARYREFAGAK
                     SELERQSEGRDKTGVFTGAFAINPTNDEQIPIFVADYVLMGYGTGAIMAVPAHDQRDF
                     EFAREFSLPIVPVIRPTDEWLAEHEALPHDCATWDEAFSGDGVAINSANDEVSLDGLR
                     VADAKVRINEWLERAEWGAPTITYKLRDWLFSRQRYWGEPFPIVYDDLGPIALPEEEL
                     PVVLPDVTDFEPVTSDDPDAEPQPPLARATDWVDVELDLEGEAWRGYGGGARTYTRET
                     NTMPNWAGSCWYYLRYLDPTNEDALVDPAVEVAWAEGARGDGSEKIGLVDLYVGGVEH
                     AVLHLLYARFWHKVLFDLGYVSTREPFQRLYNQGYILAAAYRDARGVLVEAAEVVEEN
                     GEYLHDGTPVTREYGKMGKSLKNAVTPDDMTREYGADTLRLYEMFMGPLDASRPWNTA
                     DIVGVHRFLQRLWRNLVDEETGETRVDDAPADDETRRLLHKTIDVVRDDMALLHFNTA
                     IARLFELNNYMTTVVQQRGSMPREVAEPLVLMVAPLAPHVGEELWSRLGHTDTLAYTP
                     FPEADSALLVTDAVEVPVQIGGKVRSRVMVPVGADDATHESLARADARVAELLAATTV
                     KRVVVVPGRLVNFVT"
     gene            3930..4002
                     /locus_tag="VFX21_15920"
     tRNA            3930..4002
                     /locus_tag="VFX21_15920"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3963..3965,aa:Ala,seq:ggc)
     gene            complement(4020..>4358)
                     /locus_tag="VFX21_15925"
     CDS             complement(4020..>4358)
                     /locus_tag="VFX21_15925"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5097515.1"
                     /translation="SGRQSMVSLAASASMDIGAVSRQVRELEELGAVRRLADPDDGRV
                     ALLELTAKGRRMAENLRAVGIRHLDEALRDWSVADARTLTALLGRLVDDLVATPVPAG
                     SVAPTRRPRR"
BASE COUNT          669 a         1518 c         1478 g          693 t
ORIGIN      
        1 agcggctccc gcccgctccc ccagaacgat ccccgcgacg agtgcgccca acgcgacgag
       61 cggcgcaccg tcgaaacgag cgccggtcat cccgaaacgg tgaccagctc gcgcagctgg
      121 tcgagccgcg cgtcgccgat gccgcgtacg cgcccgaggt cgtcgacgct cttgaacggg
      181 cccttctcgc gctcggcgac gatcgccgcc gccagcgacg gcccgatgcc cggcagcgtc
      241 tcgagctgcg cctcggttgc ggtgttgacg tcgatcggac ccgtcggccc gccatcagca
      301 gcgacggtgt cgccgagtgc cggtgccggg tcgggttcgc cgacgcgcgg taccgcgatg
      361 cgctggccgt cggtgacgag cgcggccagg ttcaagcgct gaaggtcggc gtcgtcggtc
      421 gcgccgcccg cggcaccgat cgcgtccacg acgcgcgcgt gcgcaccgag ctcgacgact
      481 cccggcgcgc gcaccgcccc gacgacgtgc acgacggccg gcgccgcgac cgcggtcgtc
      541 gtcgtcacga aagctgcgtt cgcctcggcc ggtggacgag caggagtctc gtcctgtccg
      601 gcgcgccaca acgcgacgcc cgcggagagc gcgacgaccg cgacggcccc gagcgcgaac
      661 cttgggttac gacgggtctg ctgcacccac tgcagcaccg gatgcggctc cggtgccggc
      721 gggcgcggga cgacatcgac ctcgggaccg gcttcgactt ccgacacggg gaacctccga
      781 ccgcgtgcgc gacgacgacg cgggggaagt tctggaggaa gtgccgcgac cctaccccga
      841 ggggcgggtc aggtcacgaa gttcacgagg cggccgggga cgacgacgac gcgcttcacc
      901 gtagtggccg ccagcaattc ggcgacgcgg gcgtcggcgc gcgccaacga ctcgtgcgtc
      961 gcgtcgtcgg cgccgaccgg caccatgacg cggctgcgga ccttgccgcc gatctgcacc
     1021 ggcacctcga ccgcgtccgt gaccagcaac gccgaatcgg cctcggggaa cggcgtgtac
     1081 gccagcgtgt cagtgtgacc caaccgcgac cacagctcct cgccgacgtg cggcgcgagt
     1141 ggcgcgacca tcaggacgag cggctccgca acctcgcgcg gcatcgagcc acgctgctgt
     1201 acgaccgtcg tcatgtagtt gttcagctcg aacagccgcg cgatcgcggt gttgaagtgc
     1261 agcaaagcca tgtcgtcgcg cacgacgtcg atcgtcttgt gcagcagacg ccgcgtctcg
     1321 tcgtcggcgg gtgcgtcgtc gacgcgcgtc tcgcccgttt cctcgtcgac caggttgcgc
     1381 cacaggcgct gcaggaaccg gtggacaccc acgatgtcgg ccgtgttcca cggccgcgac
     1441 gcatcgagcg gccccatgaa catctcgtac aggcgcagcg tgtcggcgcc gtactcgcgc
     1501 gtcatgtcgt cgggggtgac cgcgttcttc aggctcttcc ccatcttccc gtactcgcga
     1561 gtgaccggcg tgccgtcgtg gaggtactcg ccgttctcct cgacgacctc cgccgcctcg
     1621 accaacacac cgcgcgcgtc gcggtacgca gccgcgagga tgtagccctg gttgtagaga
     1681 cgttggaagg gttcgcgcgt cgagacgtag ccgaggtcga acaacacctt gtgccagaac
     1741 cgcgcgtaca gcaagtgcaa cacggcgtgc tcgacaccgc cgacgtagag gtcgacgaga
     1801 ccgatcttct cggagccgtc gccgcgtgca ccttcggccc acgcgacctc gacggcgggg
     1861 tcgacgagcg cgtcttcgtt ggtcggatcg aggtagcgca ggtagtacca acacgagccg
     1921 gcccaattcg gcatggtgtt cgtctcgcgc gtgtacgtgc gcgcgccgcc gccgtagccc
     1981 cgccacgcct caccttcgag gtcgagctcg acgtcgaccc agtcggttgc gcgcgccaac
     2041 ggcggctgcg gctcggcgtc ggggtcgtcg gaggtcaccg gttcgaaatc ggtgacgtcc
     2101 ggcagcacga ccgggagttc ttcttcgggc agcgcgatcg gaccgaggtc gtcgtagacg
     2161 atcgggaacg gttcgcccca gtagcgctgg cggctgaaca gccagtcccg caacttgtag
     2221 gtgatcgtcg gcgcgcccca ctcggctcgc tccagccact cgttgatgcg caccttcgcg
     2281 tcggcgacgc gcagaccgtc gagcgacact tcgtcgttgg ccgagttgat cgcgacgccg
     2341 tcgcccgaga acgcttcgtc ccatgtcgcg cagtcgtggg gcagcgcctc gtgctcggcc
     2401 agccactcgt cggtcggtcg gatgacggga acgatcggca gcgagaactc acgagcgaat
     2461 tcgaagtcgc gctggtcgtg cgcgggcacc gccatgatcg cgccggtgcc gtagcccatc
     2521 agcacgtagt cggcgacgaa gatcgggatc tgctcgtcgt tcgtcggatt gatcgcgaac
     2581 gcgccggtga acacacccgt cttgtcgcgc ccttcgctct ggcgttcgag ctcggacttc
     2641 gcgcccgcga actcgcggta gcgcgcaatc gcatcggcgg gcgtgccaac cgcaccgaag
     2701 aggcccttcc agtcgtcgag cacgttgctg ccgacgtcga cgaagatgtc gtcgcccggc
     2761 cattcgttgc cggcgatgtg ctcgacgagc gggtgctcag gcgcgagcac catgtacgtc
     2821 gcgccgaaca acgtgtcggg acgcgtcgtg aacacttcga tctcgtggtt ctcagggccg
     2881 acgaccggga agcgcacggt cgcgccggtg ctgcgcccga tccagttgcg ctgcatgagc
     2941 ttgatcgagt cggtccaatc gagctgttcg aggtcgtcga gcagccgatc ggcgtaggcc
     3001 gtgatccgca tcatccactg cttcagcgga cgccggtaca cggggaagtt gccgcgttcg
     3061 ctccggccgt cggcggtgac ctcttcgttg gcgagcaccg tgccgagccc cgggcaccag
     3121 ttgaccggca cctcggcgac gtacgcgagc cggaacgagt cgaccagctc gcgctgggca
     3181 gactcgtcga gcgcgtcgaa cgcgccgccg gtcggtgtcg cgcgtgtgcc cgcgcggaac
     3241 tcgtcgacga gctcggcgat cggccgggcc ttgtcttgcg cggtgtcgta ccaggagttg
     3301 aagatctgca agaagatcca ctgcgtccag cggtaatacg cgggatccgt ggtcgcggga
     3361 ccgcgccggg ggtcgtggcc gatgcccagc gcgcgcagct gcgccttcat cgtcgcgatg
     3421 ttctggtcgg tcgtgacgcg cgggtgtgtc cccgtctgca ccgcgtactg ctcggccggc
     3481 aaaccgaacg cgtcatagcc cattgcgtgc agcacgttga aaccattcat gcgctggaag
     3541 cgcgcgtaca cgtcggtcgc gatgaagccg agcggatgac cgacgtgcag ccccttgcca
     3601 ctcgggtacg ggaacatgtc gagcacgaag aggctcgggc ggtcggcgat gccgcgcggg
     3661 tcctcggcga gcagcccggt gcggttcggc gtccagaaca ctcgctcgga ctcccagtat
     3721 tcctgccagc gctgctcgat ctcgttcgcc agcgtcgcgt cgtaccggaa tttcggcgtg
     3781 tcatcggtcg cttggccctc ggcaggactc atgttcatgg ggctacagac tccggccctc
     3841 gatcaccgtt catcgacatc gtgcacaccg ggcctccggc ttgcgccggg gtcggacaaa
     3901 ccaggttact ggtacgatcg ctcccccacg ggggcgtagc tcagttggta gagcgcttgc
     3961 atggcatgca agaggtagag ggttcgatcc ccttcgcctc cactccgacc gctcagtgtt
     4021 cagcggcgag gtcggcgggt cggcgcgacg gacccagccg ggactggcgt cgcgacgagg
     4081 tcgtcgacaa gccggccgag aagtgccgtc aaggtccgag cgtcagcaac cgaccagtcg
     4141 cgcaaggcct cgtcgaggtg ccgaatgccg acggcccgca aattctcggc catgcgtcgt
     4201 cccttggcag tgagttccag caacgcaacg cgtccatcgt cgggatcggc caaccgacgc
     4261 accgcaccga gctcctcgag ttcgcgcacc tgccggctca ctgcgccgat gtccatcgac
     4321 gccgaggccg ccagagaaac catcgactgc cgaccgct
//
LOCUS       DASUGA010000810         3008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_112274, whole genome shotgun sequence.
ACCESSION   DASUGA010000810 DASUGA010000000
VERSION     DASUGA010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112274"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..2046
                     /locus_tag="VFX21_15930"
     CDS             244..2046
                     /locus_tag="VFX21_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017573175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5097516.1"
                     /translation="MKKLRARQEWAFFGALHRAAPAMAVWWWVLLVLRGVIPAMLAIA
                     IGVLVGAINRGDDLAIPLTFVGITFVSMQVLVPIHQAVSSNLGSRMSAWLNDRLMDAC
                     VTPPGMAHLERPELTSDLTIARDFDLGIMGPPLFVSMDFIAIGLATMVAGLASAALLF
                     GYSLWAPFVLAAAWLSTHWLLRESAVWKDRNTEEVRDAQRHADYAFRLAVDAPAAKEV
                     RLFGLAPWVIERFANRRRQLYELQWRATRLREKSVLGCLAIVLGANLLVFWALADAAG
                     DGRLGLGAVVVYMQAAVGVSAIAFGGLSWALDGAAAPVAAVMRLEAAMAPVGALPLGT
                     RAATGMPARDIRFRGVTFGYESSAAPVLADFDLTIPAGTSLAIVGQNGAGKTTLAKLL
                     CRLYDPQAGAIAIDGVDLRELDLDDWRSRVSAVFQDFVRYELTLRDNVAPRGADDDVI
                     EAALRDAGAVDLASLDTILARGYEGGTDFSGGQWQRVALARALCSVRLGAGVVLLDEP
                     TAQLDVRGEAEIFERILSATRHTTTILISHRFSTVRLADRICVLEHGRVVELGSHDEL
                     MELHGRYRTMFELQASRFVELDEHGAEVVYDTLA"
     gene            2030..>3008
                     /locus_tag="VFX21_15935"
     CDS             2030..>3008
                     /locus_tag="VFX21_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020519513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5097517.1"
                     /translation="MTRSRDDLPPSMSSLWRTVKIGYRAEPGMLVAAFTVVAFTALPD
                     ALIAVWLGLLANGVLHDNQDRIYLAAAGLGLSATLTWYLKVVFDRLQRRFRDRIGIAL
                     ESHVAQLQASVATIEHHERPEYLDRLAVLRDQVFALDHLFMSLFSTVGWIIRLVITIF
                     LLVAVSPVLVLLVVFALPTVMSASWRPGVERNVEERVAPNNRLARHLFVLGTTAPPGK
                     EIRVAGVGAQISEQRRASWDEWYAPVAATRWSSALWHTVAWAIFGAAYVAAIVFVAEG
                     LEKPAGAVLLVLAAGSRLSQYVGSTVGEIGFLRGIWLDSSRRLAWLEDYA"
BASE COUNT          396 a          993 c         1074 g          545 t
ORIGIN      
        1 aggtggggat ggacctcgtc ggcttcgccg acgaacccgt gttccgcggg accgagcgct
       61 cgatcttcgc ggtcccggcg agatcttgaa acggtcggcg cggcggcatt gtggacggga
      121 atcgcgatga ccagaatctc cccgtcggag ttcgggcctg ccggacgatg aaccggatgt
      181 gcgagggttc gccgtcctgt gaggacgccg cggagccacg gacggcgcag tgagggagtg
      241 ttcatgaaga agttgcgagc gcgccaggaa tgggcattct tcggtgccct gcaccgcgcc
      301 gcgcccgcga tggcggtgtg gtggtgggtg ctgctcgtcc tgcgcggcgt catccccgcg
      361 atgttggcga tcgcgatcgg tgtgctcgta ggcgcgatca accgcggcga cgacctcgcg
      421 atcccgttga cgttcgtcgg catcacgttc gtgtcgatgc aggtgctcgt accgatccac
      481 caagcggtga gctccaatct cggcagccgc atgtcggcat ggctcaacga ccgtctgatg
      541 gacgcgtgcg tgacgccgcc cggcatggcg cacctcgagc ggcccgagct caccagtgac
      601 ctcacgatcg cgcgtgactt cgacctcggc atcatgggtc cgccgctgtt cgtatcgatg
      661 gacttcatcg cgatcgggct cgcgacgatg gtggccgggc tcgcgtcggc tgcgctgctg
      721 ttcggctatt cgttgtgggc cccgttcgtc ctcgcggccg cgtggctgtc cacccactgg
      781 ctcttgcggg agagcgcggt ctggaaggat cgcaacacgg aggaggtgcg cgacgcgcaa
      841 cgccacgcgg actatgcgtt ccggctcgcg gtcgatgcgc cggccgccaa ggaggtgcgg
      901 ttgttcgggc tcgcgccgtg ggtcatcgag cgcttcgcca accgccgccg tcagctgtac
      961 gaactgcagt ggcgcgcgac gcgactgcgc gagaagtcgg tgctcgggtg tctcgcgatc
     1021 gtgctcggtg cgaacctgct cgtgttctgg gcgctcgccg acgcggccgg tgacggacgg
     1081 ctcggactcg gcgcggtcgt cgtctacatg caggcggcag tgggtgtgag cgcgatcgcg
     1141 ttcggcgggt tgagctgggc cctcgacggc gcggcggcgc ccgtggccgc ggtcatgcgg
     1201 ctcgaagcgg cgatggcccc ggtcggcgcg ctgccgctcg ggacgcgcgc cgcgaccggc
     1261 atgcctgcgc gtgacatccg gttccgcggg gtgacgttcg gctacgagtc gagtgcggct
     1321 cccgtactcg ccgacttcga cctcaccatc ccggccggta cgtcgctcgc gatcgtgggc
     1381 cagaacggcg cggggaagac gacgctggcg aagctgctct gccgcctcta cgacccgcaa
     1441 gcaggcgcga tcgcgatcga cggcgtcgac ttgcgcgagc tggacctcga cgactggcgc
     1501 tcgcgcgtga gcgcggtgtt ccaggatttc gtgcgttacg agctgacatt gcgcgacaac
     1561 gttgcgcctc gtggcgccga cgacgacgtg atcgaagcag cgctgcgcga cgccggtgcg
     1621 gtcgatctcg cgtcgctcga cacgatcctg gcgcgcggat acgagggtgg taccgatttc
     1681 tccggcggtc agtggcaacg cgttgccctc gctcgcgcgc tgtgctcggt gcgcctcggc
     1741 gccggcgtcg tgctcctcga cgagcccacc gcgcaactcg atgtgcgcgg cgaagcggag
     1801 atcttcgagc gcatcctctc ggcgacgcgg cacacgacga cgatcctcat ctcgcaccgc
     1861 ttctcgacgg tcaggctcgc cgaccgcatc tgcgtgctcg agcacgggcg cgtcgtcgag
     1921 ctcggttccc acgacgagct gatggagttg cacggtcgct accgcacgat gttcgagctc
     1981 caggcgtcgc gcttcgtgga gctcgacgag cacggtgcgg aggtggttta tgacacgctc
     2041 gcgtgacgac ctcccgccgt cgatgtcgtc gttgtggcga acggtgaaga tcgggtaccg
     2101 cgccgaaccg ggcatgctcg tcgccgcgtt cacggtggtc gcgttcaccg cgctccccga
     2161 cgcgttgatt gcggtgtggt tggggctgct cgccaatggc gtgctccacg acaaccagga
     2221 ccgcatctac ctggccgcgg ccggactcgg tctgtcggcg accttgacgt ggtatctcaa
     2281 ggtcgtcttc gaccggctgc aacggcgatt ccgcgaccgc atcgggatcg cgctggagtc
     2341 acacgtcgcg caactgcagg cgtccgtcgc gacgatcgag catcacgaac ggcccgagta
     2401 cctcgaccgg ctcgcggtgc tgcgcgatca ggtgttcgcg ctcgaccacc tgttcatgtc
     2461 gttgttctcg acggtcgggt ggatcatccg cctcgtcatc acgatcttct tgctggtcgc
     2521 ggtatcaccg gtgttggtcc tgctcgtcgt gttcgcgctg ccaaccgtca tgtcggcgtc
     2581 gtggcggccg ggcgtcgaac gcaacgtcga ggaacgcgtc gcacccaaca accgcctcgc
     2641 tcgccacctg ttcgtgctgg gcacgacggc gccgcccggc aaggagatcc gcgtcgcggg
     2701 tgtgggcgcg cagatcagcg aacagcgccg cgcgtcgtgg gacgagtggt acgcgccggt
     2761 cgcggcgacg cgttggtcga gcgcgctctg gcacacggtc gcgtgggcga tcttcggcgc
     2821 cgcatacgtc gccgcgatcg tgttcgtcgc ggagggactc gagaagccgg ccggcgcggt
     2881 gttgctcgtc ctcgcggccg gcagccggct ctcccagtac gtcggttcca cggtcggcga
     2941 gatcgggttc ttgcgcggta tctggctcga ctcgtcgcgc cggctcgcct ggctggagga
     3001 ctacgccg
//
LOCUS       DASUGA010000811         4917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_477715, whole genome shotgun sequence.
ACCESSION   DASUGA010000811 DASUGA010000000
VERSION     DASUGA010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477715"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VFX21_15940"
     CDS             complement(<1..333)
                     /locus_tag="VFX21_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097518.1"
                     /translation="MSMKRLAALALAGLCIAATACSGDDTAGTTDVASATTTSSSPAP
                     AADATTATTTAAAPTTAVDLCPYRGLGTWVDVYDTLPAFAERGRVSPVTPADVASMAE
                     HGVHTLYLQ"
     gene            complement(330..1787)
                     /locus_tag="VFX21_15945"
     CDS             complement(330..1787)
                     /locus_tag="VFX21_15945"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HEX5097519.1"
                     /translation="MPEEVSRRALLRRGGALASAAFVAPRFDLPFLRHPPAVGDRRLA
                     PDIIRRAQWGADEARRGIAEYDAVIEKIVVHHTGTENKVASWVDQVRRIYENEVASDY
                     RDIAYHYLVDPKGRIYEGRWARKYAADDPPDGEDERGWNVRGGHALRHNPRALGIALL
                     GDYTKVAPTDRALDAILDVLAWKCARWHIDPVGETPYCATDGTVETLPNIVPHGQVRV
                     TECPGPYLERALDELRDRTAARVGHQEVAGGYRVVGAGGRELAFGDADRLHDDAPAPV
                     AGIVTSPGGGGYWAFGPDGGVFAYGSAAFHGSLAGQTLNGPIVGLAPAPGNDGYWTVA
                     ADGGVFAFGNAAFAGSVAGQALRASVCGIAPSPSGRGYWCAAADGGVFAFGDAPVLGS
                     LANTQLSAPVTAIVAAGNGFWLAAADGGVFAFGDAPYFGSGTPGAAVVAMTPTTTGQG
                     YYLLTGAGDVVPFGDATYHGSANGRLPDAVGIAPA"
     gene            complement(1876..2997)
                     /locus_tag="VFX21_15950"
     CDS             complement(1876..2997)
                     /locus_tag="VFX21_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018894732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HEX5097520.1"
                     /translation="MNNMSRRDFVRVAGGGTLLLAVGSAGLLAACDPGALRAPDANGL
                     QLQPGFTSRIIATTGQPVASTGYVWHASPDGGACFALADGGWSYVSNCESIPGGAGFV
                     RFASDGTITGAGSCLTGTIVNCAGGGTPWGTWLSCEEHPAGQVWECDPLGATAGVARP
                     AMGRFSHEAVAVDAANQCVFLTEDRSDGGLYRFSPATWPNLASGTLAILTETAGALAW
                     VTVPDPSGAIGTTRTQVPNTKHFNGGEGAAMSDGRLIFTTKGDNRVWSYDPAANALRV
                     VYDPAVKVNGVLRGVDNVTSSSTGVIYVAEDGGDMQIVLVRNNGDTYAVVQVTGVTGS
                     EITGPAFDPSGTRLYFSSQRNPGRTYEVAGPWSRFASRL"
     gene            3457..>4917
                     /locus_tag="VFX21_15955"
     CDS             3457..>4917
                     /locus_tag="VFX21_15955"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX5097521.1"
                     /translation="MGYDAFISYSHAGDGQLAPAVQSGLQRMARPWYRRRALHVFRDE
                     TGLSVNPDLWQAIRAALDDSEWFVLFASPEAAQSEWVNLEVETWLDAKPVERVLPVIT
                     GGTVEWDAARNDFTPDSDAVPPALRGMYADQPRWLDLRWARTADDLDLRNAGFRLAIA
                     GLAAPMHGMPKDEFEGEDIRLHRRAMRFARLGIATLVVFAVAAIAASVLAFGYAQRAN
                     ASADRERRSAAAARAARADADALAANERDAQDAATAAAENANAQQDRAETEAQKAKQA
                     QDEAEAAQADAEAEADNARQAQATADASAQDAKQQAARADRQATVAQTQGSRADAEAQ
                     RANDAATAAQQNADEARANAAEASANAQTAAENAAEAKRIADDLATANTSLAATNDQL
                     VDERNLSGRQRVAAVANRLFAEAGAAAANHDDAISSLLAVQGQAFAARTAAAGGGGKS
                     ALGRAGDTPAEGLVSEDAITTGLATALTDNPALLQVP"
BASE COUNT          735 a         1751 c         1791 g          640 t
ORIGIN      
        1 ctgcaggtac agcgtgtgca cgccgtgctc ggccatgctc gcgacgtcgg ctggtgtgac
       61 gggagagacg cggccgcgct ccgcgaacgc gggcagcgtg tcgtacacgt cgacccacgt
      121 ccccagcccg cggtacgggc acaggtcgac ggcggtcgtc ggggccgcgg cggtggtcgt
      181 cgcggtcgtc gcgtcggccg cgggagcggg tgatgacgat gtcgtcgtcg cgctcgcgac
      241 atcagtcgtg cccgcggtgt cgtcaccgga gcacgcggtc gcggcaatgc acaggcccgc
      301 gagcgcgagc gccgcgagcc gtttcatgct catgcgggcg cgatgccgac cgcgtcgggg
      361 agccgcccgt tggccgagcc gtggtacgtg gcgtcgccga agggcacgac gtcacccgcg
      421 cccgtgagca ggtagtagcc ctgcccggtc gtcgtcggcg tcatcgcgac cacggctgcg
      481 cccggggtgc cgctgccgaa gtacggcgcg tcgccgaacg cgaagacacc gccgtcggcg
      541 gcagcgagcc agaagccgtt gccggcggcg acgatcgcgg tgaccggcgc ggacagttgg
      601 gtgttcgcga gcgagccgag cacgggcgcg tcgccgaatg cgaagacgcc gccgtcggcg
      661 gcggcgcacc agtacccgcg cccggacggg ctcggcgcga tgccgcagac cgatgcgcgg
      721 agcgcctggc cggcgacgga gccggcaaag gcggcgttgc cgaacgcgaa gacgccgccg
      781 tcggccgcga cggtccagta gccgtcgttg ccgggcgcgg gcgcgaggcc cacgatcggc
      841 ccgttcagcg tctggcccgc gagcgaaccg tggaacgcgg ccgagccgta cgcgaacacg
      901 ccgccgtccg ggccgaacgc ccagtagcca ccgccgcccg gagacgtcac gatgcccgcg
      961 accggtgccg gcgcgtcgtc gtgcagccga tcggcatcgc cgaacgcgag ctcgcgtccg
     1021 ccggcgccga cgacgcggta accaccggcg acctcttgat gcccgacgcg cgccgccgtc
     1081 cggtcgcgca gctcgtcgag cgcccgctcg aggtacgggc cgggacactc ggtgacgcgc
     1141 acctggccgt gcggcacgat gttcggcaag gtctcgacgg tgccgtcggt cgcgcagtac
     1201 ggcgtctcgc cgaccggatc gatgtgccag cgcgcgcact tccacgcgag gacgtcgagg
     1261 atcgcgtcga gcgcgcggtc ggtcggcgcg accttcgtgt agtcacccaa gagcgcgatc
     1321 ccgagcgcgc gcggattgtg cctgagcgcg tggccgccgc gcacgttcca gccgcgctcg
     1381 tcctcaccgt cgggcgggtc gtcggccgcg tacttgcgcg cccagcgtcc ttcgtagatc
     1441 cgacccttcg ggtcgacgag gtagtggtac gcgatgtcgc ggtagtcgga cgccacttcg
     1501 ttctcgtaga tgcgccgcac ctgatcgacc cagctcgcga ccttgttctc ggtgccggtg
     1561 tggtgcacga cgatcttctc gatgaccgcg tcgtactcgg cgatgccgcg gcgcgcttcg
     1621 tcggcacccc attgcgcgcg acggatgatg tcgggcgcga gccgccggtc accgaccgcg
     1681 ggcgggtggc gcaggaaggg caggtcgaag cgcggcgcga cgaacgccgc actcgcgagc
     1741 gcgccgcccc gacgtagcaa cgcccgccgc gacacttctt caggcaaccc gtccgcctcc
     1801 cgcgtccctg cagcaactgc gcccgcgtgg cgagggtacc ggagcggttc ttcgttcagg
     1861 tcgcggtgcg cgcgctcaga gccggctggc gaatcgcgac cacggccccg caacttcgta
     1921 ggttcggccc gggttgcgct gcgagctgaa gtacaaccga gtgcccgacg ggtcgaacgc
     1981 gggcccggtg atctccgacc cggtcacgcc ggtcacctgc acgaccgcgt acgtgtcgcc
     2041 gttgttgcgc acgaggacga tctgcatgtc gccaccgtcc tcggcgacgt agatgacccc
     2101 ggtgctggag gaggtgacgt tgtcgacgcc gcggagcacg ccgttgacct tcacggccgg
     2161 gtcgtagacg acgcgcagcg cgttggcggc ggggtcgtac gaccacacgc ggttgtcgcc
     2221 cttggtcgtg aagatcaatc ggccgtcact catggccgcg ccttcaccac cgttgaagtg
     2281 cttcgtgttc ggcacctgcg tgcgcgtcgt cccgatcgcg cccgacgggt ccggcaccgt
     2341 cacccacgcc agcgcgccgg ccgtttccgt gaggatcgcg agtgtgccgg acgcgaggtt
     2401 cggccacgtc gccggtgaga agcggtacaa gccgccgtcg ctgcggtcct cggtgaggaa
     2461 gacgcactga ttggcggcgt cgaccgcgac tgcttcgtgg gagaagcgcc ccatcgccgg
     2521 gcgcgcaaca ccggccgtcg cgccgagcgg atcacactcc cagacctggc cggcgggatg
     2581 ctcctcgcaa ctcagccacg tgccccacgg cgtcccgcca cccgcgcagt tcacgatcgt
     2641 gcccgtcagg caactcccgg cgccggtgat cgttccgtcc gacgcgaagc gcacgaagcc
     2701 cgcgccaccc ggaatggact cgcagttgct cacgtacgac caaccgccgt cggcgagcgc
     2761 gaagcacgcg ccgccgtcgg gtgacgcgtg ccagacgtaa ccggtgctcg caacgggctg
     2821 gccggtcgtc gcgatgatgc gcgacgtgaa gcccggttgc agctggagac cgtttgcgtc
     2881 gggcgcccgc aacgcgccgg gatcgcaggc ggcgagcaat ccggcactgc cgaccgcgag
     2941 gagaagcgtg ccgccgcccg cgacgcggac gaagtcgcgg cgagacatgt tgttcatact
     3001 cgcgacggta cgcgagtggc actcggcgcg gtcgttgtaa gggcggcggc aatgtgaccg
     3061 ccttgccccg cttgcacaga tccggaggct tcccgccctc cggcccgggc gtgccctggg
     3121 caccggtcgg gacgacgaag ccgggggtgc cccgcacttt gcggcggaag ttgccggggt
     3181 cgaggcgcgg cccgacgcgg cttcgtacgc ggcgcgcagc tcggacagcg tgaacgtctt
     3241 gccgacgaag gcagccgcga tcgaggtgta ctcgagcttc gcgcgcgcaa cggccgcttc
     3301 gaggatcgtg cggtgatcga acgcgagcgc accttcgaac ggaggcagcg cgcgctcgac
     3361 cagcagcacg gccagctcct cgtcatccgg cgcccctggc cacgccctcc agggtgagga
     3421 aggatcttcc acgtctgcag tgcacggagg tcgttcatgg gttacgacgc cttcatctcc
     3481 tacagccacg cgggggacgg gcagctggcg ccggcggtgc agtccggcct ccagcgcatg
     3541 gcgaggccgt ggtaccggcg gcgcgcgctc cacgtgttcc gcgacgagac cgggctgtcg
     3601 gtcaaccccg atctgtggca ggcgatccgc gccgcactcg acgactccga atggttcgtg
     3661 ctgttcgcat cgccggaagc cgcgcagtcg gagtgggtca acctcgaagt cgagacgtgg
     3721 ctcgacgcca agcccgtcga acgcgtcctc cccgtcatca ccggcgggac cgtcgagtgg
     3781 gacgcagcgc gcaacgactt cacgcccgac tccgacgcgg tgccgccggc gctgcgcggc
     3841 atgtatgccg atcagccgcg ttggctcgac ttgcggtggg cgcgcaccgc tgacgatctc
     3901 gacctgcgca atgcgggatt ccgactcgcg atcgcgggac tggccgcgcc gatgcacggt
     3961 atgcccaagg acgagttcga aggtgaagac attcgtctgc accggcgcgc gatgcgcttc
     4021 gcccgactcg gcatcgcgac gctcgtggtg ttcgcggtcg cggcgattgc cgcgagcgtg
     4081 ctggcgttcg ggtacgcgca acgtgcgaac gcgtctgcgg accgggaacg gcgctcggcc
     4141 gcggccgcac gcgcggcccg tgcggatgcc gacgcgctgg ctgcgaacga gcgcgatgcg
     4201 caggacgcgg ccacagccgc agccgagaac gcgaacgcgc agcaggaccg cgccgagacc
     4261 gaggcgcaga aggccaagca ggcgcaggat gaggccgaag cggcgcaggc cgacgccgaa
     4321 gcggaggccg acaacgcccg ccaggcgcag gccaccgccg acgcgagcgc acaggacgcc
     4381 aagcagcagg ctgcgcgcgc ggaccggcag gccacagtag cgcagacaca gggttcccgg
     4441 gccgacgccg aggcgcaacg cgcgaacgat gcggcgactg ccgcgcagca gaacgcggac
     4501 gaggcgcgcg ccaacgcggc cgaagcgtcc gcgaacgcgc agaccgcggc cgagaatgcg
     4561 gccgaggcga agcggatcgc cgacgacctg gcgaccgcga acacatcgct cgccgcaacc
     4621 aacgaccaac tcgtcgacga acgcaatctc agcgggcgtc aacgcgtcgc ggcggtcgcg
     4681 aaccggctgt tcgcggaagc gggagccgcc gccgccaacc acgacgacgc gatctcgtcg
     4741 ctgctcgccg tgcagggtca ggcgttcgcg gcgcgtacgg ccgccgcggg cggcggaggc
     4801 aagtcggcgc tcggtcgcgc gggcgacacg ccggcggagg gactcgtcag cgaggacgcg
     4861 atcacgaccg ggctcgcgac ggcactgacc gacaaccccg cgttgctgca agttccg
//
LOCUS       DASUGA010000812         4509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_615086, whole genome shotgun sequence.
ACCESSION   DASUGA010000812 DASUGA010000000
VERSION     DASUGA010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615086"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VFX21_15960"
     CDS             <1..820
                     /locus_tag="VFX21_15960"
                     /inference="COORDINATES: protein motif:HMM:NF015164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CobD/CbiB family cobalamin biosynthesis protein"
                     /protein_id="HEX5097522.1"
                     /translation="GTVMTRVERSIYRDERMAGVAYAAIGTAIGAGAGLVLRRRGAAV
                     ALAAAGRELRRASARVRDSLLRDDLDDARMNVSALVGRDPSDLDASGLSAAVIESLAE
                     NSVDAVVAPTLWALVAGAPGALGYRAVNTMDAMVGHHSPRYEHFGWAAARLDDVANYV
                     PARTTALLVAAVRPRRAPEVWRAVRTQGVAHPSPNAGVAEASFAAALGCELGGPLRYG
                     DRPEVRPKMGFGPRPQPADITRAITLANHVELALLTGVAGAELLRFSAARRRRT"
     gene            817..1821
                     /gene="cobT"
                     /locus_tag="VFX21_15965"
     CDS             817..1821
                     /gene="cobT"
                     /locus_tag="VFX21_15965"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEX5097523.1"
                     /translation="MRLEADAMDAARARLDRLAKPPGSLGRLEDLAVQLAGLAGTCPP
                     PTPANPAIAVFAGDHGVVASGVTPWPQDITAAMIGVFARGGAAINVFAHQVGATVYVV
                     DVGVAGDVSAHGDVMHRKVRAGTDDLGRGPAMTVADAQAAIDVGKDIARELIGRGHDL
                     LVTGDMGIGNTTSAAALIAAFTGRDAADVTGRGTGIDDAMLGRKIEIVAAAVAAAAPN
                     VESLALLSRLGGLEIAALAGYIVEACTARVPLVVDGVIACAAALVADAIAPGVRDLVI
                     AGHRSTEPGASIALAHLGLEPLVDLELRLGEGTGAALAIPLVQTAARLLTEMATLDDL
                     "
     gene            complement(1830..2171)
                     /locus_tag="VFX21_15970"
     CDS             complement(1830..2171)
                     /locus_tag="VFX21_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HEX5097524.1"
                     /translation="MPPRGVRKGSKRARQYQHIKSSAKKRGASTGRAEEIAARTVNKE
                     RARSGESRTRSKSSTRGKSPSARGGQRSGTNRPKGRTREQLYEEAKRKNIKGRSRMSK
                     AQLQRAVAAKK"
     gene            complement(2260..2343)
                     /locus_tag="VFX21_15975"
     tRNA            complement(2260..2343)
                     /locus_tag="VFX21_15975"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2307..2309),aa:Leu,seq:cag)
     gene            2452..3105
                     /locus_tag="VFX21_15980"
     CDS             2452..3105
                     /locus_tag="VFX21_15980"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HEX5097525.1"
                     /translation="MGLQRFERRLERLVEGAFTKAFRSGLQPVEIGRRLARELDAGRT
                     LGVRGTVVPNRLTVMLSAEDTERFSGFHDALVHELEDAVREHARDEDYHFVGPVEVTL
                     GVDERRRRGDLKVVAEIVADETGSVGSLVLPDGRRVRLGDDVAVIGRLPECAVALSDP
                     QVSRHHSEVRRAGNVYRVRDLGSTNGTMVNGAVIADQELHDGDEITVGNTMIRYEES"
     gene            3111..3551
                     /locus_tag="VFX21_15985"
     CDS             3111..3551
                     /locus_tag="VFX21_15985"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5097526.1"
                     /translation="MSEQVLTVLKFCLLALMYLFLARVVWVVVRELRGTPVAAPVVQP
                     PAKQSRKKTREIVVAQPDTAVYAIPFAGEATVGRGGGCAIILSGDTFVSTVHARFVEG
                     DGGVWVEDLGSTNGTFVNDKKISQPSRLKKGDRVHIGNSVLEVR"
     gene            3558..>4509
                     /locus_tag="VFX21_15990"
     CDS             3558..>4509
                     /locus_tag="VFX21_15990"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX5097527.1"
                     /translation="MELSAGAFSDPGRVRDNNEDAYVVDDRLTLFAVADGMGGHLGGE
                     VASWTAIEALRASVANRRAINVAITEANDAVRERASGDPNLNGMGTTLTAVVVAGGRL
                     LLIGHVGDSRAYLLHEGSLRRITDDHSLVEELVREGRLTPEQAESHPQRAVVTRALGV
                     DEEVDVDVFTVEVAEGDRVVLCSDGLTTMVRERDVERIMAAGNDPQRTAEALVDAANE
                     AGGTDNITVIVLDVVSVDDDTPIDPLAFAQADAPVTPVSVPAPDVEGAEPSAPAEKRS
                     RRVRNVLLLAIPLVVIVLFAVGVAGYYARRSYYVGFEGDTV"
BASE COUNT          636 a         1585 c         1598 g          690 t
ORIGIN      
        1 cggcacggtg atgacgcgcg tcgagcgctc gatttaccgg gacgagcgca tggcgggtgt
       61 cgcgtacgcc gcgatcggca ccgcgatcgg cgcgggcgcg ggactggtgt tgcgacgccg
      121 cggcgcagcc gtcgcgctcg ccgccgcagg cagagagctg cggcgcgcgt ccgcgcgcgt
      181 gcgcgactcg ttgctgcgcg acgacctcga cgacgcgcgt atgaacgtct ccgcgctcgt
      241 cggccgcgac ccgagcgacc tcgacgcgtc ggggctgagc gctgcggtga tcgagtcgct
      301 ggcggagaac agcgtcgacg cggtcgtcgc gccgaccttg tgggcgctcg tggccggtgc
      361 tccgggcgcg ctcggatacc gcgcggtcaa cacgatggac gcgatggtcg ggcatcactc
      421 gccgcgctac gagcacttcg gttgggcggc ggcgcgtctc gacgacgtcg ccaactacgt
      481 ccccgcccgc acgaccgcgc tgctcgtcgc ggcggtgcgc ccccgccgcg cccccgaggt
      541 ctggcgcgcg gtgcggaccc agggcgttgc gcacccctcg cccaacgccg gcgtcgcgga
      601 agcctcgttc gccgcggcac tcggctgcga gctcggcggc ccgctgcgct acggcgaccg
      661 ccccgaggtc cgcccgaaga tgggcttcgg gccgcgcccc caaccggccg atataacccg
      721 cgcgatcaca ttggcgaacc acgtcgagct cgcgttgctg acgggcgtcg ccggtgcgga
      781 actattgcgg ttcagcgccg cacggaggag acggacgtga gactcgaagc ggacgcgatg
      841 gacgcggcgc gggcgcggtt ggaccggctc gcgaagccgc cgggttcgct cgggcgactc
      901 gaggacctcg cggtgcagct cgcgggtctg gccggcacgt gcccgccacc aactccggcc
      961 aaccctgcga tcgcggtgtt cgccggcgac cacggggtcg tcgcgtcggg agtgacgccg
     1021 tggccgcaag acatcacagc ggcgatgatc ggcgtgttcg cgcgcggcgg cgcggcgatc
     1081 aacgtcttcg cgcaccaggt cggcgcgacc gtctacgtcg tcgacgtcgg cgtcgcgggc
     1141 gacgtcagtg cgcacggcga cgtcatgcac cgcaaagttc gcgcgggcac cgacgacctc
     1201 gggcgcggcc cggcgatgac tgtcgccgac gcgcaggccg ccatcgacgt cgggaaggat
     1261 attgcacgcg agctgatcgg ccgcgggcac gacctcctcg tgaccggcga catggggatc
     1321 ggcaacacga cgtccgccgc cgcgctgatc gcggcgttca cgggacgtga cgcggccgac
     1381 gtcaccggtc gcgggacggg tatcgacgac gcaatgctcg gtcgcaagat cgagatcgtc
     1441 gcggcggccg tcgccgccgc ggcacccaat gtcgaatcgc tcgcgttgct ctcccggctg
     1501 ggcggcctcg agatcgcggc gctcgcgggt tacatcgtcg aggcctgtac cgcgcgcgtt
     1561 ccgctcgtcg tggacggcgt catcgcctgc gcggcggcgc tggtggcgga cgcgatcgcg
     1621 ccgggggtcc gcgatctcgt gatcgcgggg caccggtcga ccgagccggg cgcgtcgatt
     1681 gcgctcgcgc acctgggtct ggagccgctc gtcgacctcg aactgcgcct cggtgagggc
     1741 acgggtgccg ccttggccat cccgctcgtg cagacggcgg cccgcctcct caccgagatg
     1801 gcgaccctcg acgacctgta gcggtcggct tacttcttgg cggccaccgc tcgttggagc
     1861 tgcgctttgc tcatccgcga gcggcccttg atgttcttgc gcttggcctc ttcgtagagc
     1921 tgttcgcggg tgcggccctt gggccggttg gtgccggaac gctggccgcc gcgcgccgac
     1981 ggcgacttgc cgcgcgtcga cgacttcgac cgcgtgcggg actcacccga acgcgcgcgc
     2041 tccttgttga cggtgcgcgc cgcgatctcc tcggcgcgac cggtcgacgc gccccgcttc
     2101 ttcgcgctcg acttgatgtg ctggtactga cgggctcgtt tggagccctt ccgcactccc
     2161 ctcggtggca tggctggtcc tttcaacggg gaagttgacg cttcgcgcga tacccgtttt
     2221 cgccacacct caatatgagg gtcaccgcat ctgaaagggt gcgcgagggg ggacttgaac
     2281 ccccacgccc ttgcgggcac aggaacctga atcctgcgcg tctgccaatt ccgccactcg
     2341 cgcgtggagt gagaagggta ccagccgcct gcacaattac ttcacgtaac cccgctccca
     2401 cccggcggag tcgcacaaga aatgcacaag cccgggacta tcgtcgcacc gatgggactc
     2461 cagcggttcg agcgtcggct cgagcggctc gtggagggtg ccttcaccaa ggcgttccgc
     2521 agcggcctgc aaccagtcga gatcggacgg cgtctggcgc gcgagctcga cgcgggccgc
     2581 acgctcggcg tgcgcggcac cgtggtcccc aatcgcctga ccgtcatgtt gtcggccgag
     2641 gacaccgaac ggttctccgg cttccacgac gcgctcgtgc acgagctcga agacgcggtg
     2701 cgcgagcacg cgcgcgacga ggattaccac ttcgtcgggc cggtcgaagt gacgctcggc
     2761 gtcgacgagc gccggcggcg cggcgacctg aaggtggtcg ccgagatcgt cgccgacgag
     2821 acggggtccg tcggttcgct ggtcctcccc gacggacggc gcgtccgtct cggtgacgac
     2881 gtcgccgtca tcggccgcct gccggagtgt gccgtcgccc tctccgaccc gcaggtctcc
     2941 cgccatcact cggaggtccg ccgcgccggc aacgtgtacc gggtccgtga tctcgggtcc
     3001 acgaacggca cgatggtgaa cggcgcggtg atcgccgacc aggagctgca cgacggcgac
     3061 gagatcaccg tcggcaacac gatgatccgc tacgaggagt cctaagtcac ttgtccgaac
     3121 aagtgctgac tgtcctcaag ttctgtctgc tcgcgttgat gtacctgttc ctcgcgcgcg
     3181 tcgtgtgggt cgtcgtgcgc gagctgcgcg gcacgccggt cgccgcgccc gtcgtgcaac
     3241 cgccggcgaa gcagagccgc aagaagaccc gcgagatcgt cgtcgcgcag cccgacaccg
     3301 ccgtgtacgc gatcccgttc gccggcgagg cgacggtcgg gcgcggtggt gggtgcgcga
     3361 tcatcctctc cggtgacacg ttcgtgtcga cggtgcacgc ccgcttcgtc gaaggcgacg
     3421 gtggcgtgtg ggtcgaggat ctggggtcga cgaacggcac gttcgtcaac gacaagaaga
     3481 tctcgcagcc gtcgcgcttg aagaagggtg accgcgtgca catcggcaac tcggtgctcg
     3541 aggtcaggta agtcgcaatg gagctctccg ccggcgcctt cagcgatccc ggtcgcgtcc
     3601 gcgacaacaa cgaggacgcg tacgtcgtcg acgaccgcct cacgctgttc gcggtcgccg
     3661 acgggatggg tggccacctc ggcggtgagg tcgcgtcgtg gaccgccatc gaagcgctgc
     3721 gcgcatcggt cgccaaccgc cgtgccatca acgtcgcgat caccgaagcc aacgacgcag
     3781 tacgcgaacg cgcgagcggc gatccgaacc tgaacggcat gggcacgacg ctcacggccg
     3841 tcgtcgtggc cggcggccgc ttgctgctca tcgggcacgt cggcgactcg cgggcgtacc
     3901 tcctgcacga gggttcactg cgccgcatca ccgacgacca cagcctcgtc gaggagctcg
     3961 tgcgcgaagg gcggctcacg cccgagcaag ccgagagcca tccgcagcgc gccgtcgtga
     4021 cacgcgcgct cggtgtcgac gaagaggtag acgtcgacgt gttcaccgtc gaagtagccg
     4081 agggcgatcg cgtggtgctc tgttccgacg gactgacgac gatggtgcgc gaacgcgacg
     4141 tcgagcgcat catggccgcc ggcaacgacc cgcagcgcac cgcggaggcg ttggtcgacg
     4201 ccgcgaacga ggccggcggc accgacaaca tcacggtcat cgtgctcgac gtcgtctcgg
     4261 tcgacgacga cacgccgatc gatccgctcg cgttcgcgca agccgacgcg cccgtcaccc
     4321 cggtgtcggt gcccgcgccc gacgtcgagg gcgcggagcc gtcggcgccg gcggagaagc
     4381 gcagccgccg ggtgcgcaac gtcctgctcc tcgccattcc cctcgtcgtg atcgtgctgt
     4441 tcgcagtggg cgtcgcgggc tactacgcgc gccgctcgta ctacgtcggg ttcgagggcg
     4501 acacggtcg
//
LOCUS       DASUGA010000813         4460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1207647, whole genome shotgun sequence.
ACCESSION   DASUGA010000813 DASUGA010000000
VERSION     DASUGA010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207647"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..360)
                     /locus_tag="VFX21_15995"
     CDS             complement(163..360)
                     /locus_tag="VFX21_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097528.1"
                     /translation="MPRKGRARRFREARKLKQGFDDGLFREGSGTLADLEDGVADDDD
                     DFDDDDDWDDDDWDEDDPLKD"
     gene            complement(397..1059)
                     /locus_tag="VFX21_16000"
     CDS             complement(397..1059)
                     /locus_tag="VFX21_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006372812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX5097529.1"
                     /translation="MKTTAAFDELARTMRAQAPRRRLTTEPVDDATVLGLLELASHAV
                     AGRPRRCEFVVVRDPDVRHQLARMYRQGWSVYKRILRSRASADATLAARQWEADHFED
                     VPVIIVTCVRGRRPLFPAIGAATFYGTAFAAVQNLLLAAQAAGLGAGVTTLALWSGWE
                     ARRTLGLPRHVTPVAVVPIGWPRGGAEAGPIAPIGNLVHLDRFGHQPFRSPSAERGSD
                     MA"
     gene            complement(1070..2029)
                     /locus_tag="VFX21_16005"
     CDS             complement(1070..2029)
                     /locus_tag="VFX21_16005"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HEX5097530.1"
                     /translation="MAVTMAPEADLLDDFVPTARLTDKARPAPGWREELRRIPNARNA
                     ISVASCWVQTLAIIWVAVWWNHPVGWVLAFLLMGRAHAQFAALMHEAAHRLLFSNKKV
                     NDWVGRWLLGYPSFTPIDLYRRGHMAHHREEFGPEEPDIPLYRGYPIPRDSFRRKLWR
                     DATGRTGWKLFKNLLRGAKAPAPAGTQARRIIGVQLVLIAASIALGHPWVYWLLWFVP
                     YLTVWRVINRLRSIAEHGGMKQSKDRRETTHSVRQHWPARFMMVPFHIGWHLAHHVDS
                     GVPMKHLPRYHAELVRSRYVTDALEYGSYPLLWRKLSSGEKPA"
     gene            2059..2847
                     /locus_tag="VFX21_16010"
     CDS             2059..2847
                     /locus_tag="VFX21_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX5097531.1"
                     /translation="MTGLSNIPAQRPLTPRSLIASLLLGMQPPRLPGALLVRWCGAFG
                     VAEGTARVAMSRMVERGELAAADGVYELAGRVRARQAPQDWSVDPELERWDGAWRLAT
                     VTSAARAAADRAALRDTFRQLRYAEYREGVWCRPHNVPRVGAPESAWAVAEAQCAFWT
                     GRPDEEAHALAARLFDPIGWAGQARDLTARLDATTKTLRAPSDDDLAAAFTTGAAALA
                     HIRHDALLPPPLCPKPWPGTELRASYRRYQRTFAAAARTWFRAA"
     gene            2888..>4460
                     /locus_tag="VFX21_16015"
     CDS             2888..>4460
                     /locus_tag="VFX21_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097532.1"
                     /translation="MRRMCTFGWVAIAGWFVVAATAAHAQVPVEIRALDACLFVDHND
                     SGNYANADGTPLTTPSVFGLDLSFDNFPPFGPADRYVVQVTVDGPEGTLSGTGPLAPD
                     GRARVPIGISSFGAYSPVGAVVTDQNNAAYTIDLGPVWVDPFVVDANEVNCDFDALTT
                     GAPADVTDTPTSAPTTTAAVVTDDTGASSTGATTTTTEKDDGPPWIPIIIAGAVISTT
                     GLFLLTRNRGKTEDPESAIDGILQGFEGTSVDQPPAMTADDCWEAWQRAVAIYADDRA
                     ALLEKYAAMLDGFTGQFGTAMGGLDTFRENYGALLSASSEMQAAVQRWTEVQSVSKKT
                     DLVVTGVTLTVGAGSLAIKGGKWVATKFATTGAELTAEAIAAQRTAAGVAVGAQKLEQ
                     AVNAATNINKALAAAPMTNTMQPSAVVITQLAKEAGRDLAAVIAESGGIEAATEKLFW
                     EVFAKRGYHFLPNAGAMAAKFEKPTYMFGKLINSIPKELIAEGFGDAGQVLPRLVANL
                     RITLGKMAGGGAAAAE"
BASE COUNT          691 a         1513 c         1611 g          645 t
ORIGIN      
        1 ccagcccgtc gtcacggcgg cggtccccga gctcacgggc gagcccgcgg ccgcgttcgg
       61 agagccggct gtgctgccag ccggatgcaa tgccgcggtc gttgtcgacg ctgagggaat
      121 gggtttcgaa gacgaccgac agggccatcg ggccgttgcg gactagtcct tcagcgggtc
      181 gtcctcgtcc cagtcgtcgt cgtcccagtc gtcgtcgtca tcgaagtcgt cgtcgtcatc
      241 ggcaacgccg tcttcgagat cggcgagggt gccagacccc tcgcgaaaca acccgtcgtc
      301 gaagccctgc ttgagcttgc gcgcttcgcg gaacctgcgt gctcgtcctt ttctgggcac
      361 gctgtcacct gcctcggagc ggaaatcgtg cccccatcat gccatgtcgc tcccgcgctc
      421 ggcggagggg gagcgaaagg gctggtggcc gaagcggtcc aagtggacga ggttgccgat
      481 gggtgcaatc gggcccgcct ccgcgccgcc ccgcggccat ccgatcggca cgaccgcgac
      541 gggagtgacg tggcgcggca ggccgagtgt ccggcgcgcc tcccatcccg accacagcgc
      601 cagggtcgtc acgccggcgc cgagtcccgc ggcttgtgcc gcaaggagga gattttgcac
      661 cgccgcgaac gcggtgccgt agaacgtcgc cgcgccgatc gcggggaaca gcggtcggcg
      721 accgcgcacg cacgtcacga tgatgacggg cacgtcctcg aagtggtcgg cttcccattg
      781 gcgggccgcg agcgtcgcgt cggcgctggc gcgcgagcgc aggatccgct tgtagaccga
      841 ccatccctgg cggtacatgc gggccagttg gtggcggacg tcggggtcgc ggacgacgac
      901 gaactcgcac cgtcgcgggc ggccggcgac ggcgtgactg gcgagctcga gcagcccgag
      961 gacggtcgcg tcgtcgacgg gttcggtggt gagccgccgc cggggtgcct gggcgcgcat
     1021 ggtgcgcgct agctcgtcga aggccgctgt cgtcttcacc gcgcggaggc taggccggct
     1081 tctcgcccga gctgagcttg cgccacagca gcgggtacga gccgtactcg agtgcgtcgg
     1141 tgacgtatct cgagcgcacc aactcggcgt ggtaacgggg gaggtgcttc atcgggacgc
     1201 cggagtcgac gtggtgggcg aggtgccagc cgatgtggaa cggcaccatc atgaaccgcg
     1261 ccggccagtg ctgccgcacg gagtgcgtcg tctcgcggcg atccttcgac tgcttcatgc
     1321 cgccatgctc ggcgatcgaa cgcaggcggt tgatcacgcg ccagacggtc aggtacggca
     1381 cgaaccacag cagccagtac acccacgggt gaccgagcgc gatcgacgcc gcgatgagca
     1441 cgagctgcac gccgatgatg cgccgcgcct gcgtgcccgc gggcgcgggt gccttcgcgc
     1501 cgcgcagcaa gttcttgaac agcttccagc cggtgcggcc ggtcgcgtcg cgccacagct
     1561 tgcggcggaa ggagtcgcgc gggatcgggt agccgcggta cagcgggatg tcgggttctt
     1621 ccggcccgaa ctcctcgcgg tgatgagcca tgtggccgcg gcggtagagg tcgatcggcg
     1681 tgaacgacgg gtagccgagc aaccagcggc cgacccagtc gttgaccttc ttgttgctga
     1741 acaacaaccg gtgcgcggct tcgtgcatga gtgccgcgaa ctgtgcgtgc gcgcggccca
     1801 tcagcaagaa cgcgagcacc catcccacgg ggtggttcca ccagaccgcg acccagatga
     1861 tcgcgagcgt ctgcacccag cacgacgcga ccgagatcgc attgcgcgcg ttggggatac
     1921 ggcgcagctc ctcacgccac ccgggcgcgg gacgggcctt gtcggtcagg cgcgcggtgg
     1981 ggacgaagtc gtcgagcaga tccgcttccg gagccatggt cacggccatc gggacccgat
     2041 attacactgc cacgacagat gacgggactt agtaatatcc cggcccagcg gccgctcacg
     2101 ccccggtcgc tgatcgccag cctgctgctc ggcatgcagc cgccccggtt gccgggcgcg
     2161 ctgctcgtgc ggtggtgcgg tgcgttcggc gtcgccgagg gcaccgcacg cgtcgcgatg
     2221 agccgcatgg tcgagcgcgg cgagctcgcc gccgccgacg gtgtgtacga gctggccggg
     2281 cgcgtgcggg cccggcaggc accgcaggat tggagcgtcg accccgagct cgagcgatgg
     2341 gacggtgcct ggcggctcgc gacggtgacc tcggcggccc gcgcggccgc ggatcgcgcc
     2401 gcgctgcgcg acacgttccg ccagctgcgc tacgccgagt accgcgaggg cgtgtggtgc
     2461 cggccccaca acgtcccgcg cgtcggcgcg cccgagagcg catgggcagt cgccgaagcg
     2521 cagtgcgcgt tctggaccgg ccggcccgac gaggaagcgc acgcgctcgc ggcgcggctg
     2581 ttcgacccga tcggttgggc gggccaggcc cgcgacctga ccgcacggct cgacgcgacg
     2641 accaagacgc tgcgggcacc aagtgacgac gatctcgcgg ccgcgttcac gaccggcgcg
     2701 gccgcgcttg cgcacatccg ccatgacgcc ttgctgccgc cgccgctgtg cccgaaaccc
     2761 tggcccggca ccgagctgcg cgcctcgtac cggcggtacc agcggacgtt cgcggccgcg
     2821 gcccggacat ggttccgcgc cgcctgaccg gcttctaggc tctcgcgaac tcggcgggga
     2881 gcgagacatg cggcggatgt gtacgttcgg gtgggtcgcg atcgcgggat ggttcgtcgt
     2941 cgccgcgacg gccgcgcatg cgcaggtgcc ggtggagatc cgcgcgctgg acgcatgtct
     3001 cttcgtcgac cacaacgact ccggcaacta cgcgaacgcc gatggcacgc cgttgacgac
     3061 gccttcggtg ttcggcctcg acctgtcgtt cgacaacttc ccgccgttcg gtccggcgga
     3121 tcgctacgtc gtccaagtca ctgtcgacgg accggaaggc accctgtccg gcacgggccc
     3181 gctcgcgccc gacggacggg cacgggtgcc gatcgggatc agttccttcg gtgcgtactc
     3241 gccggtgggt gccgtcgtca ccgaccagaa caacgcggcc tacacgattg acctcggacc
     3301 ggtgtgggtg gatcccttcg tcgtcgacgc gaacgaagtc aactgcgact tcgacgcact
     3361 gaccaccggt gcaccggctg atgtcaccga cacgccgacg agtgcgccga cgacgacggc
     3421 ggcggtggtc accgacgaca ccggcgcgtc gtcgacgggc gcgacgacca cgacgaccga
     3481 gaaggacgac ggcccgccgt ggatcccgat catcatcgcg ggcgccgtca tcagcaccac
     3541 gggcctgttc ctgctgacgc gcaatcgcgg caagaccgag gatcctgaga gcgcgatcga
     3601 cggcatcctg cagggcttcg aaggcacgtc cgtcgaccaa ccgccggcga tgaccgccga
     3661 cgactgctgg gaagcgtggc agcgcgcggt ggcgatctac gccgacgacc gagccgcgct
     3721 gttggagaag tacgcggcga tgctcgacgg gttcaccggg cagttcggca ccgcgatggg
     3781 cgggctcgac acgttccgcg agaactacgg cgcgttgctg agtgcgagca gtgagatgca
     3841 ggccgcggtg cagcggtgga ccgaagtgca gtcggtgtcg aagaagaccg acctcgtcgt
     3901 cacgggtgtc acgctgaccg tcggcgcggg gagccttgcg atcaagggcg gcaagtgggt
     3961 cgcgacgaag ttcgcgacga ctggcgccga gttgaccgcg gaggcgatcg ccgcgcagcg
     4021 caccgccgca ggtgtggccg tcggcgcgca gaagctcgaa caagccgtga acgcggccac
     4081 caacatcaac aaggcgctcg cggccgcgcc gatgacgaac acgatgcagc cgtccgcggt
     4141 ggtcatcacg cagctcgcga aggaggccgg tcgcgacttg gcggccgtca tcgccgagag
     4201 cggcggtatc gaagccgcca ccgagaagct gttctgggag gtgttcgcca agcgcggcta
     4261 tcacttcctg cccaacgcgg gcgcgatggc agccaagttc gagaagccca cgtacatgtt
     4321 cgggaagctc atcaacagca tcccgaagga gctgatcgcc gaaggcttcg gcgacgccgg
     4381 acaggtgctg ccgcgcctcg tcgcgaactt gcgcatcacg ctggggaaga tggccggcgg
     4441 tggtgcggcc gcggccgaag
//
LOCUS       DASUGA010000814         3679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1502326, whole genome shotgun sequence.
ACCESSION   DASUGA010000814 DASUGA010000000
VERSION     DASUGA010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502326"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="VFX21_16020"
     CDS             complement(<1..1162)
                     /locus_tag="VFX21_16020"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5097533.1"
                     /translation="MSTTTNELPADPTVPGFYLRPDYYDLLADLRRDAPVHEFAPGLR
                     TIARYEDIREISRDPARFCSSMGALVNDPARTWEKPMKAPSILHMDPPDHVGFRKLVN
                     REFTPRAIAKLEPRIRELARTTLDRTPDEGLFDFVDLVAAPFPLLVIAELLGIPDGDR
                     REFREWSDAMIEATDKSQADVAAGTGELFRYLMEHIEAKRADPGGDVVSLLVDAEVDG
                     RSLDPDELLIFLLALLVAGNETTRTLISGGTLTLFEHPDQRADLATDAALVPGAVEEC
                     LRWVTPIQAFARTVTADTVIGGVEVNERDYLIMLYASGNRDEAAFGPTANVFDIRRDV
                     NPPHLAFGFGEHLCLGAALARMEARILFEEMLARHPEYVVAGEPEWVASSLVR"
     gene            complement(1159..2316)
                     /locus_tag="VFX21_16025"
     CDS             complement(1159..2316)
                     /locus_tag="VFX21_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097534.1"
                     /translation="MSELGPLGPLDELLAHQIVDTFATVSQSDRSWTEKICAMAAARD
                     GSLALGFGLGKYPNRNVMDAYAGVSRGTEQWTVRASRELGADDATAVGPITYGVLEPM
                     RCIRFALAANDVQPVSFEWTFEGVVPAVLENHEVHRSRDGHRVDADIARYHHIGTAHG
                     WVDVDGDRTEIAPGTWVSTRDHSWGVRYQVGVPPTDIAPAPMPASVATTVIWSPILMT
                     RADGTHYGLHTYYQRHAFGEYQRIELQGGFEHADGRREHYAALVPELDVDPVNRRLRG
                     GVLHATMSDGTARDLTITVLSATGFHLGAGLYFGFDGHWHGEWRGRTHIDGEYIADCT
                     TAEQARRLHQLRDCIVRVDDPGGGGIGIGNLQSLVAGPHPEIGLDEETSFM"
     gene            2345..3349
                     /locus_tag="VFX21_16030"
     CDS             2345..3349
                     /locus_tag="VFX21_16030"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX5097535.1"
                     /translation="MALLTHRDPLAVGAALAGWLTEHRGVAGARVVEARHPSIGYSSE
                     TVMVDLEWEGGSGQFVVRLAPPAAGTFRDYDLAEQVAAQLAAARAGVAVATPELVTDP
                     RWLEAPFVVMPRVDGHIIGEVAAFDAWLLSLTVEQRAEVHESFVAAIATTHRAQPPAG
                     VPARDNLAELAFWADYLNWSSSDAPLPGLVAALQWCHGHQPATESAPVLLWGDVRLGN
                     VIFGDDRRPRAVLDWDMATIGAREHDVAWFVMLETTMRAMLGRTLEGFPDRDGTIAQY
                     QRLSGHTLQDFEWYETFALLRSTAIMTRIGYLYEAAGQRPPLPIADNPLLDLLAARIA
                     "
     gene            complement(3346..>3679)
                     /locus_tag="VFX21_16035"
     CDS             complement(3346..>3679)
                     /locus_tag="VFX21_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097536.1"
                     /translation="EMQQTYARHLLDSDRDAMIGPTLGSATEAAGFHVVDRRVITAMP
                     PADVLANGVRPPGWAARDAMIASGHATPADVERWDVALTRFAALAAVGRAALFIPMYT
                     VVARKPAA"
BASE COUNT          547 a         1218 c         1339 g          575 t
ORIGIN      
        1 cgcgcacgag actggacgca acccattccg gttcgccggc aacgacgtat tcgggatggc
       61 gcgccagcat ctcttcgaac aggatgcgcg cctccatgcg ggcgagcgcg gcaccgaggc
      121 agagatgttc accgaacccg aacgcgagat gcggcgggtt gacgtcgcga cggatgtcga
      181 acacgttcgc ggtcgggccg aacgcggctt cgtcgcggtt gccggacgcg tacagcatga
      241 tcaagtagtc gcgctcgttg acttcgacgc caccgatgac ggtgtcggcg gtcaccgtgc
      301 gagcgaacgc ctggatcggc gtcacccagc gcaagcactc ctccaccgcg cccgggacga
      361 gcgcggcgtc ggtggcgaga tcggcgcgct ggtcggggtg ctcgaacaac gtgagggtgc
      421 cgcccgagat cagcgtgcgc gtcgtctcgt tgcccgcgac caacagggcc agcagaaaga
      481 tcagcagttc gtcgggatcg agtgaccggc cgtcgacctc ggcatcgacg agcaacgaca
      541 cgacgtcgcc gccgggatcc gctcgtttgg cttcgatgtg ctccatcagg tagcggaaca
      601 gctcgccggt cccggccgcg acgtcggcct gcgacttgtc ggtcgcctcg atcatcgcgt
      661 cggaccactc gcggaactcg cggcggtcgc cgtcggggat gccgagcagc tcggcgatga
      721 cgaggagagg gaacggcgcg gcgacgaggt cgacgaagtc gaacagacct tcgtcgggcg
      781 tgcggtcgag cgtggtacgc gccaactcgc ggatgcgcgg ctcgagcttc gcgatcgcac
      841 gcggggtgaa ctcgcggttc acgagcttgc ggaagccgac gtggtcgggc ggatccatgt
      901 gcaggatcga cggcgccttc atcggcttct cccacgtgcg cgccgggtcg ttcacgagcg
      961 cgcccatcga ggagcagaac cgcgccgggt cgcggctgat ctcgcggatg tcttcgtagc
     1021 gtgcgatcgt ccgcaggccg ggtgcgaact cgtgcaccgg cgcgtcgcgc cgcaggtccg
     1081 cgaggaggtc gtagtagtcc gggcggagat agaagccggg aacggtcggg tcggccggga
     1141 gttcgttcgt cgtggtgctc acatgaatga cgtttcttcg tcgagtccga tctcgggatg
     1201 cgggcccgcc accaggctct gcaagttacc gatgccgatg ccgccgccgc ccggatcgtc
     1261 gacgcgcacg atgcagtcgc gcaattggtg caggcggcgg gcctgctcgg cggtcgtgca
     1321 gtctgcgatg tattcgccgt cgatgtgggt gcggccgcgc cattcgccgt gccagtgacc
     1381 gtcgaatccg aagtacaagc ccgcgccgag atggaacccg gttgcggaca gcacggtgat
     1441 cgtgaggtcg cgcgcggtgc cgtcgctcat ggtggcgtgc agcacgccgc cgcgcaggcg
     1501 ccggttcacc gggtcgacgt cgagctcggg gacgagcgcg gcgtagtgct cgcggcgtcc
     1561 gtccgcgtgc tcgaagccac cttgcagctc gatgcgctgg tactcaccga acgcgtggcg
     1621 ttggtagtag gtgtgcaacc cgtagtgcgt gccgtcggcg cgggtcatca ggatcggcga
     1681 ccagatcacc gtcgtcgcca cactcgccgg catcggcgcg ggcgcgatgt cggtcggtgg
     1741 cacgccgact tggtagcgca cgccccacga gtggtcgcgc gtcgagaccc aggtgccggg
     1801 cgcgatctcg gtgcggtcgc cgtcgacgtc gacccaaccg tgcgcggtgc cgatgtggtg
     1861 atagcgcgcg atgtcggcgt cgacgcgatg gccgtcgcgc gaacggtgaa cctcgtggtt
     1921 ctcgagcacc gcgggtacga caccctcgaa cgtccactcg aacgagaccg gctgcacgtc
     1981 gttggccgcg agcgcgaagc ggatgcaacg catcggttcg aggactccgt aggtgatcgg
     2041 tccgaccgcg gtcgcgtcgt cggcaccgag ctcgcgactg gcgcggaccg tccactgctc
     2101 ggtgccgcga gagacgcccg catacgcatc catgacgttg cgattcgggt acttgccgag
     2161 gccgaagccg agggccagcg aaccgtcgcg cgcggccgcc atcgcacaga tcttctcggt
     2221 ccacgagcgg tcggactgcg agacggtcgc gaacgtgtcg acgatctggt gcgcgagcag
     2281 ctcgtcgagc gggccgagtg gtccgagttc actcacgcgt gtgaatgtac tcaggaggtt
     2341 cgtcatggcg ctcctgaccc atcgtgatcc actcgccgtc ggcgccgcgc tggccggttg
     2401 gctgaccgag caccgcggcg tcgcgggcgc gcgcgtcgtc gaggcgcggc atccgtcgat
     2461 cggctattcg agtgagacgg tgatggtcga cctcgagtgg gagggcggtt cggggcagtt
     2521 cgtggtgcgg ctggcgccgc cggccgcagg cacgttccgc gactacgacc tggccgaaca
     2581 ggtcgccgcg cagctcgccg cggcgcgagc cggcgtcgcc gtcgccacgc cggagctggt
     2641 gaccgacccg cgttggctgg aggcgccctt cgtcgtgatg ccgcgggtcg acgggcacat
     2701 catcggcgaa gtcgcggcgt tcgacgcgtg gttgctctcg ctgacggtcg agcagcgagc
     2761 ggaagtgcac gagtcgttcg tcgccgcgat cgcgacgacg catcgggcgc aaccacccgc
     2821 gggtgtcccc gctcgcgaca acctcgccga gctcgcgttc tgggccgact acctgaactg
     2881 gtcgagcagc gacgcgccgc tcccgggact cgtcgccgcg ctgcagtggt gccacggcca
     2941 tcagcccgcg acggaatcgg cgccggtctt gctgtggggc gacgttcgtc tcggcaacgt
     3001 catcttcggc gacgaccgcc gtccgcgcgc ggtgctcgac tgggacatgg cgaccatcgg
     3061 agcgcgggag cacgacgtcg cgtggttcgt catgctggag acgacgatgc gcgcgatgct
     3121 cggccgcact ctcgaggggt tccccgatcg cgacggcacg atcgcgcagt accaacgcct
     3181 gagcggacac acgctccagg acttcgagtg gtacgagacg ttcgcgctgc tgcgcagcac
     3241 cgcgatcatg acgaggatcg gctacctcta cgaagccgcg ggccaacgcc cgccgctccc
     3301 gatcgccgac aacccgctgc tcgacctgct ggcggcccgg atcgcctaag cggcgggctt
     3361 gcgggcgacc acggtgtaca tggggatgaa cagcgcggcg cggccaacgg ctgcgagggc
     3421 ggcgaagcgc gtgagcgcga cgtcccagcg ttcgacgtcc gcgggtgtcg cgtgaccgct
     3481 ggcgatcatc gcgtcccgtg ctgcccagcc cggagggcgc acaccgttgg ccaacacgtc
     3541 ggccggtggc atcgcggtga tgacccggcg gtcgacgacg tggaagccgg ccgcctcggt
     3601 tgcggagccg agcgtcggcc cgatcatcgc gtcgcgatcg gagtcgagca gatggcgcgc
     3661 gtacgtctgc tgcatctcc
//
LOCUS       DASUGA010000815         2216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_374584, whole genome shotgun sequence.
ACCESSION   DASUGA010000815 DASUGA010000000
VERSION     DASUGA010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374584"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="VFX21_16040"
     CDS             complement(<1..1248)
                     /locus_tag="VFX21_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018502862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5097537.1"
                     /translation="MRQTLPHMSHWGAFRVTVENDTIVEVEGHPDDPAPSPLLANYLD
                     GIRNESRVRKPAVRKGWLDRGPGPDPHRGADDFVEVEWDEALDLVAGELRRVRTHFGD
                     EAIYGGSYGWASAGRFHHAQSQIHRFLNCIGGYTASWHSYSLGASEVILPHVVGSADA
                     VLRRATTWNAILEHTELVVAFGGMNVKNAWVSPGGVTRHTLPPSLAEAGRRGLIFELF
                     SPLRSDLIPEVNAHWHPVVPGTDTAAMLALAYCLDADGLVDRDFLARYCVGGDQLLAY
                     VRGDEDGVAKTPEWAAAITGIDASVFPELARRMAAHRTLVTVSWSLQRTRHGEQAPWM
                     GIALAALLGQIGLPGGGFGHGYGSMADVGAPRVPYSLPVFRQGRNPVTKFIPVAQITR
                     LLENPGGTLDYDGRALPLPDIRVV"
     gene            1307..>2216
                     /locus_tag="VFX21_16045"
     CDS             1307..>2216
                     /locus_tag="VFX21_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011210886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5097538.1"
                     /translation="MDFELPADLVAYLAELDEFIETEIKPLEAQNDNIRFFDHRREDA
                     RTDWDRGGLPNDEWEMLLHDVKRRADAAGHYRYPFPKEYGGKDGTNLGMAVIREHLAR
                     KGLGLHNDLQNEHSIVGNNVGLLLMINYGTDEQKAEWIDALAEGRRGFAFGITEPSHG
                     SDATHMETSAVRDGDEWIINGEKTWNTGIHKAKYDLIFARTSGKPGDGDGITAFLTPT
                     DAPGFEIVEFLWTFNMPTDHAHIRLTDVRVPHSAIFGGEGTGLQVTQHFFNENRIRQA
                     AASLGAAQFCINEAVKYAMERKPFGKP"
BASE COUNT          384 a          744 c          754 g          334 t
ORIGIN      
        1 cacgacgcgg atgtcgggga gcggcaacgc gcggccgtcg tagtcgagtg tgccgccggg
       61 gttctccagc aggcgcgtga tctgcgcgac cgggatgaac ttggtgaccg ggttgcggcc
      121 ctggcggaac accggcaacg agtacggcac gcgcggcgcg ccgacgtcgg ccatcgatcc
      181 gtacccgtgc ccgaagccgc cgccggggag accgatctgc ccgagcagcg cggcgagcgc
      241 gatgcccatc cacggcgcct gctcaccatg gcgggtgcgc tgcagtgacc agctcacggt
      301 gacgagcgtg cggtgcgcgg ccatgcggcg tgcgagttcc gggaacaccg acgcgtcgat
      361 gccggtgatc gcggccgccc actcgggcgt cttcgccacg ccgtcttcgt cgccgcgcac
      421 gtacgcgagc agttggtcgc cgccgacgca gtagcgcgcg aggaagtcgc ggtcgacgag
      481 cccgtcggcg tcgaggcagt acgcgagcgc aagcatcgcg gcggtgtcgg tcccgggcac
      541 gactggatgc cagtgcgcgt tcacttccgg aatgaggtcg ctgcgcaacg ggctgaacag
      601 ttcgaagatg aggccgcgcc gtcctgcttc cgcaaggctc ggtggcaacg tgtggcgcgt
      661 gaccccgccg gggctgaccc acgcgttctt cacgttcatg ccgccgaacg cgacaacgag
      721 ctcggtgtgc tcgagaatcg cgttccaggt cgtcgcgcgc cgcaacactg cgtcggcgct
      781 cccgaccacg tgcgggagga tgacctccga ggcgccgagc gagtagctgt gccacgaggc
      841 ggtgtagccg ccgatgcaat tcaggaagcg gtgaatctgg ctctgcgcgt ggtggaagcg
      901 gcccgcgctc gcccagccgt aggagccgcc gtagatcgcc tcatcgccga agtgggtgcg
      961 cacgcgacgc aactcgcccg cgacaaggtc gagcgcctcg tcccactcga cttcgacgaa
     1021 gtcgtcggca ccgcggtgcg gatccggccc gggaccgcgg tcgagccagc ccttgcgcac
     1081 tgcgggcttg cgcacccgtg attcgttgcg gatgccgtcg agatagttcg ccagcaacgg
     1141 tgacggtgcg ggatcgtccg ggtggccctc gacctcgacg atggtgtcgt tctcgacggt
     1201 gacgcggaac gcgccccagt gcgacatgtg cggcaacgtt tggcgcatag gggcaggcta
     1261 cgcccgcatc tggcgccgct gctcccggtt tagggatgct ctcgggatgg acttcgaact
     1321 tcccgccgat ctggttgcct atctcgccga actcgacgag ttcatcgaga ccgagatcaa
     1381 accgctcgaa gcacagaacg acaacatccg gttcttcgac catcgccggg aggacgcgcg
     1441 gaccgactgg gatcgcggcg ggctccccaa tgacgagtgg gagatgctgc tgcacgacgt
     1501 gaagcgccgg gccgatgcgg ccggccacta ccgctatccg ttcccgaagg aatacggcgg
     1561 caaggacggc acgaatctcg gcatggccgt catccgcgaa cacctcgcgc gcaaggggct
     1621 cgggctgcac aacgacttgc agaacgagca ctcgatcgtc ggcaacaacg tcgggctgtt
     1681 gttgatgatc aactacggca ccgacgaaca gaaggcggaa tggatcgacg cactcgccga
     1741 aggccggcgc ggctttgcat tcggcatcac cgaaccctcg cacggttccg acgcgaccca
     1801 catggagacg agcgcggtgc gcgacggcga cgagtggatc atcaacggtg agaagacgtg
     1861 gaacaccggc atccacaagg cgaagtacga cctgatcttc gcccgcacgt ccggcaagcc
     1921 cggcgacggc gacggcatca ccgcgttcct cacgccgacc gatgcgcccg gcttcgagat
     1981 cgtcgagttc ctctggacgt tcaacatgcc gaccgatcac gcgcacattc gtctcaccga
     2041 cgtgcgcgtt cctcacagtg cgatcttcgg cggcgagggc accggcctcc aggtcaccca
     2101 gcacttcttc aacgagaacc gcatccgcca ggccgccgcg tcgctcggcg cggcgcagtt
     2161 ctgcatcaac gaggcggtga agtacgcgat ggagcgcaag ccgttcggca agccgt
//
LOCUS       DASUGA010000816         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1105466, whole genome shotgun sequence.
ACCESSION   DASUGA010000816 DASUGA010000000
VERSION     DASUGA010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105466"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VFX21_16050"
     CDS             <1..424
                     /locus_tag="VFX21_16050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5097539.1"
                     /translation="RERWHDAVRLARSLTGSTAAAEDIAQEVFGRMYLSWGRADEPAA
                     YLRVSLVNTCRSWHRRRELERVRLPMLVSTNPGASEPDELGDVVQALPNRQRAVVMMR
                     YWGDLSEAEIAEELGCAPGTVKSLASRARERLAVAIAS"
     gene            456..1199
                     /gene="rlmB"
                     /locus_tag="VFX21_16055"
     CDS             456..1199
                     /gene="rlmB"
                     /locus_tag="VFX21_16055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX5097540.1"
                     /translation="MKKSASTLGGEEVEGRRAVLELLRADRRRVHSVLLSGSVERDDV
                     VDEIVERAGPRLRTVTPEKLANQARSDGHQGVLANAEPLRAADFDELCEARDAFLVAL
                     DGVTDPRNLGAIMRVAETAGATGIVLPRHRSARITAAVTKAAAGAIEYLPVAVVGGIP
                     AALERAKRSGCWTAGLDERGERTLYEFDLADQPLVLVLGAEGRGLARLTRERCDMVVS
                     IPMHGHIASLNVATAAALACHEIARRRAQ"
     gene            1214..1286
                     /locus_tag="VFX21_16060"
     tRNA            1214..1286
                     /locus_tag="VFX21_16060"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1247..1249,aa:Thr,seq:tgt)
     gene            1452..1712
                     /locus_tag="VFX21_16065"
     CDS             1452..1712
                     /locus_tag="VFX21_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HEX5097541.1"
                     /translation="MDRLSGRERAMLDFEREWWQVEGRKDDQIRARFRQSPSSYYRAL
                     QALIDRDVAVEYDPLSVKRLRRRRDQRREVRMVGRRADPGSR"
     gene            1748..>2202
                     /locus_tag="VFX21_16070"
     CDS             1748..>2202
                     /locus_tag="VFX21_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097542.1"
                     /translation="MSRQMGGRGNGGGSKEVARGAGFAAAKGAGLIALAVIIGIVLLN
                     VVDDGSVNNDGASSTNTTTPTTAAATDSTGGGETTPTTVDDVPPKTPADLSVLVLNGG
                     APPRSAKSMSDALRLKDYTNQLEPADWQDHSQTGNTVMCKAGLDEEGAAL"
BASE COUNT          354 a          751 c          769 g          328 t
ORIGIN      
        1 tcgcgaacgt tggcacgacg cggtgcgact ggccaggagc ctgaccggca gcacggccgc
       61 ggccgaagac atcgcgcaag aggtcttcgg ccggatgtac ctgtcgtggg gccgagccga
      121 cgaaccggcg gcgtacctgc gcgtgtcatt ggtgaacacc tgccggtcct ggcaccggcg
      181 ccgtgagctc gagcgcgtgc ggctgccgat gctggtctcg accaaccccg gcgcgagcga
      241 acccgacgag ctgggtgacg tcgtgcaggc gctgccgaac cggcaacggg cggtcgtcat
      301 gatgcggtac tggggtgacc tctccgaggc cgagatcgcc gaagagctgg gctgcgcacc
      361 cggcaccgtg aagtcactcg cgtcacgcgc gcgcgaacgc ctcgcggtcg cgatcgccag
      421 ctagctcggc cgggcgctcg ctagcgtccg ggccgatgaa gaagtctgcg tcgacgctcg
      481 gcggcgagga ggtcgaaggc cggcgcgcgg tcctcgaact gctgcgcgcc gaccggcgcc
      541 gcgtgcattc ggtgctcctc tcgggttcgg tcgagcgcga cgacgtcgtc gacgagatcg
      601 tcgaacgggc cggcccgcgc ctgcgcacgg tgacacccga gaagctcgcc aaccaggcgc
      661 gcagcgacgg gcaccaaggc gtgctggcga acgccgaacc gctccgggcc gcggacttcg
      721 acgagctgtg cgaggcgcgc gatgcgttcc tcgtcgcgct cgacggcgtc accgaccctc
      781 gcaatctcgg tgcgatcatg cgcgtcgcgg agacggcggg cgcgaccggc atcgtgctgc
      841 cgcgccaccg cagcgcgcgc atcaccgcgg cggtgacgaa agcggcggcc ggcgcgatcg
      901 agtacctgcc ggtcgcggtc gtgggcggca tcccggccgc gctcgaacgg gcaaagcggt
      961 caggttgttg gaccgcgggg ctcgacgaac gaggcgaacg gacgttgtac gagttcgatc
     1021 tcgccgacca accgctcgtc ctcgtactcg gtgccgaagg ccgcgggctc gcacgtctca
     1081 ctcgcgaacg ttgcgacatg gtggtcagca tcccgatgca cggccacatc gcgtcgttga
     1141 acgttgcgac cgccgccgcg ctcgcgtgcc atgaaatcgc gcgccgccgc gcgcagtaat
     1201 ctttcaggat tacgccgggt tagctcagct ggcagagcag ctgatttgta atcagcaggt
     1261 cgtcggttcg attccgacac ccggctctat atttctttgt cgctctcgac ctgctcgcgt
     1321 gaggcacgtc tcgttgttgg ccggcctcga gactccgcga gtgcctccgc aagctccggc
     1381 accgccctgt gcgacgcgcg cgagtccaaa tgccgtctgt tgtcgcacct accgaataca
     1441 ttgcgtcgct gatggatcgg ttgtcggggc gtgagcgggc gatgctcgat ttcgaacggg
     1501 agtggtggca ggtcgaggga cgcaaggacg atcagatccg cgcccggttc cgccaatcac
     1561 ccagcagcta ctaccgcgcg ttgcaggcgc tcatcgaccg tgacgtggcc gtcgagtacg
     1621 acccgttgtc cgtgaagcgt ctccggcgcc gccgcgacca acggcgcgag gtgcggatgg
     1681 tcggccggcg ggccgatccc gggtcccggt aggcgcgaag ccgcccccga cgcgacagaa
     1741 tgaccgcatg agtcgccaga tgggtggtcg aggcaatggc ggcgggagca aagaagtggc
     1801 gcgcggcgcc ggctttgcgg ccgccaaggg tgccgggctg atcgcgctcg cggtcatcat
     1861 cggcatcgtg ctgttgaacg tcgtggacga cggctccgtg aacaacgacg gcgcgtcgtc
     1921 cacgaacacg accactccca cgaccgcggc tgccaccgac tccaccggtg gtggtgagac
     1981 caccccgacc acggtcgatg acgtcccgcc caagacaccg gccgacctga gcgtgctcgt
     2041 cctcaacggt ggcgcgccgc cccgttcggc caagtcgatg tcggatgcgc tgcggctcaa
     2101 ggactacacg aaccagctcg agcccgcgga ctggcaagac cacagccaaa ccggcaacac
     2161 cgtgatgtgc aaggccggcc tcgacgaaga aggcgccgcg ct
//
LOCUS       DASUGA010000817         3214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1346740, whole genome shotgun sequence.
ACCESSION   DASUGA010000817 DASUGA010000000
VERSION     DASUGA010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346740"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="VFX21_16075"
     CDS             complement(<1..155)
                     /locus_tag="VFX21_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009086148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEX5097543.1"
                     /translation="MRGSKPFARQNDCVSGAAISLFSGAGGLDLGIEEGGFQTVAAVE
                     WDADAADS"
     gene            275..745
                     /locus_tag="VFX21_16080"
     CDS             275..745
                     /locus_tag="VFX21_16080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HEX5097544.1"
                     /translation="MRGRTVALGGGKRVPYPVPKNTAATRVGKGNTRKDTTPEVRLRS
                     ALHGRGLRFRKDFVLVVEGRRVRPDIVFTRVGIAVFVDGCFWHCCPVHGTMPTSNPDY
                     WVPKLRRNVERDRQIDVDLARDGWIVVRVWEHEDAEQAAACIAARVGDRRAPAG"
     gene            complement(975..1796)
                     /locus_tag="VFX21_16085"
     CDS             complement(975..1796)
                     /locus_tag="VFX21_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX5097545.1"
                     /translation="MFKVFKRWWKYMTARLSGKFEESADPKTQLEQAIQEAQDQHRRL
                     TEQAANVIAHQKQTQMRLDRASEELEKVTANARQALLLTDEATTKGDTAKALEYQRAA
                     ESFANRLIALEREVEGLKNMLLEATQNADAAKGAVQKNSMALQKKLSERQRLLSQLDQ
                     AKMQEQMNKAMATLTATVGEDVPTFEQVREKIEARAAKAQGMADLQGTSVEARMIDVE
                     QAQLDAEAAARLSELRTQLGLPAPVSEAPAPEPTPTAAEQPAQQPATGGSGEPAG"
     gene            complement(1806..2438)
                     /locus_tag="VFX21_16090"
     CDS             complement(1806..2438)
                     /locus_tag="VFX21_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097546.1"
                     /translation="MIWLALFSLGVGIGAALAARWAWVKQRRNPMLGIDPMTLSMPWR
                     QWVKDAVSARRRFGEVVATTKPGPIRERLAEIGGGIDTAVQEVWRVARYGDELDEGIE
                     QLDLPNVTRRIERLEADGSADDAVRALKEQLASGERLRGVAADARQRLEVQDARLNEA
                     VARAIELSLSASDVSELSGLGDDVETLVGDMEALRQGLEVARAASRGQAS"
     gene            complement(2435..>3214)
                     /locus_tag="VFX21_16095"
     CDS             complement(2435..>3214)
                     /locus_tag="VFX21_16095"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5097547.1"
                     /translation="VKREVHHIVNLTRVEKLRREHGLPVPERSRHLVFVGNPGTGKTT
                     VARLLSKVYGVLQVLEKGHLVETDRGGLVSGYVGQTAPKVQEVVKSALGGTLFIDEAY
                     ALWVNSAEDFGKEAIATLLKLMEDERDKLIVVVAGYPKPMEEFLDANPGLRSRFPKTI
                     SFPDYSTDDLLQIFAALGEKEQYHAAPETLARVRSYVDAQARGPSFGNARLCRNLFEA
                     AIELHATRVVDIPDTTKEQLSTLLPDDIPAPGAVLPAEGGA"
BASE COUNT          478 a         1117 c         1043 g          576 t
ORIGIN      
        1 gaatcggccg cgtcagcgtc ccactcaacg gcggccaccg tttggaaccc gccctcttcg
       61 atgccaaggt ctaggccgcc ggcacccgag aacaaggaga tggccgctcc gctcacgcag
      121 tcattctgac gggcgaacgg cttcgatccg cgcatcctgg cgagggggag gcgtagctgc
      181 tggtcggcgc actggagttc ttctgtggat cgcttcatga cgcgtagtag accgtgaggg
      241 tgtgacagcg aagcctcgag cacgggctga agttgtgcgc ggccgaacgg ttgctctcgg
      301 cggcggcaag cgcgttccct atcccgtccc aaagaacaca gcggcgacgc gcgtcggcaa
      361 gggaaacacg cgcaaggaca ccacgcccga ggtgaggctg cggtccgcac tccacggtcg
      421 cgggctgcga ttccgcaaag acttcgttct tgtcgtcgaa ggacgaagag ttcgtccaga
      481 catcgtcttt acgcgcgtcg gaatcgcagt cttcgtcgac ggctgcttct ggcattgctg
      541 ccccgtacac gggaccatgc ccacgtcaaa tcctgactac tgggtaccca agttacgtcg
      601 caacgtcgag cgtgatcgtc agatcgatgt ggacctcgca cgggatggat ggatcgtcgt
      661 tcgagtgtgg gagcacgagg acgccgagca ggctgcggca tgcatcgcag cccgcgtcgg
      721 cgaccgacgg gcgccggcag gctgacgtat gcagcggccg cggctcgccg tggtaatccg
      781 acaccgtttc gcgcgtcact cagaacaatg aactaccacc ccgccaacta gcactcagcg
      841 gttcattgaa cgcagcgacg tcgtgagaac acctgacgac gccctcgttc gacgcgcact
      901 tcgccagccc gacgtagatc atcggtcagc acgcgcttct tgtgtcggcc tccaaccgcc
      961 gtcgaccgac ggactcagcc cgcgggttcg ccgctgccgc ccgtggccgg ctgttgcgcg
     1021 ggttgttccg cggcggtcgg cgtgggttcg ggcgccggtg cttcgctcac cggtgcgggg
     1081 aggcccaact gtgtgcgcag ctccgacagg cgcgccgcgg cttcggcatc gagttgggcc
     1141 tgctcgacgt cgatcatgcg ggcctcgacc gaggtgccct gcaggtcggc catgccctgc
     1201 gccttcgccg cgcgcgcttc gatcttctcg cgcacctgct cgaacgtcgg gacgtcctcg
     1261 ccgacggttg ccgtcagcgt ggccatcgcc ttgttcatct gctcttgcat cttcgcctgg
     1321 tcgagctgcg acagcaggcg ttgacgctcc gacagcttct tctgcagcgc catcgagttc
     1381 ttctggaccg cgcccttcgc cgcgtcggcg ttctgggtcg cctccagcag catgttcttc
     1441 aggccctcga cctcgcgctc gagcgcgatc aagcggttcg cgaacgactc cgcggcgcgc
     1501 tggtactcca gcgccttcgc ggtgtcacct ttcgtcgtcg cctcgtcggt gagcagcagc
     1561 gcctgccggg cgttggccgt gaccttctcc agctcttcgc tcgcccggtc gaggcgcatc
     1621 tgcgtctgct tctggtgcgc gatgacgttc gcggcctgct cggtgaggcg ccggtgttgg
     1681 tcttgggctt cctggatcgc ttgttcgagc tgtgtcttcg ggtccgcgga ctcctcgaac
     1741 ttcccggaca accgggccgt catgtacttc caccagcgct tgaagacctt gaacatgcga
     1801 acagcctatg acgcctgtcc acggctggcc gcccgagcga cctcgagccc ctggcgcagt
     1861 gcctccatgt cgccgacgag cgtttcgacg tcgtcaccca agcccgacag ctccgacacg
     1921 tcgctcgccg acagcgacag ctcgatggca cgagcgacgg cttcgttgag ccgtgcgtcc
     1981 tgtacttcca aacgctgacg ggcgtcggcc gcgacgccgc ggaggcgctc accggacgcg
     2041 agctgctcct tcagcgcccg gaccgcgtcg tcggccgatc catcggcttc gaggcgctcg
     2101 atgcgacggg tgacgttcgg cagatcgagc tgctcgatgc cttcgtcgag ctcgtcgccg
     2161 tagcgcgcga cccgccagac ctcctgcacc gcggtgtcga tgccgccacc gatctcggcg
     2221 aggcgctcac ggatgggccc gggcttcgtc gtcgcaacga cctcaccgaa gcgccggcgc
     2281 gcgctcaccg cgtccttgac ccactggcgc cacggcatcg acaacgtcat cgggtcgatg
     2341 cccagcatcg ggttgcgccg ttgcttcacc cacgcccaac gagcggcgag cgcggcaccg
     2401 atgcccacgc ccaacgagaa gagggcgagc cagatcacgc gccgccctcg gcgggcaaca
     2461 ccgcgccggg cgccgggatg tcgtcgggca gcaacgtgct gagctgttcc ttggtcgtgt
     2521 cggggatgtc gaccacgcgc gtcgcgtgca gttcgatcgc ggcctcgaac aggttgcggc
     2581 acaagcgcgc gttgccgaac gacgggccgc gcgcttgcgc gtcgacgtac gaccgcacgc
     2641 gcgccaacgt ctccggcgcg gcgtggtact gctccttctc gccgagcgcg gcgaagatct
     2701 gcaacaggtc gtcggtcgag taatcgggga acgagatcgt cttggggaag cgcgaccgca
     2761 gccccgggtt cgcgtcgagg aactcctcca tgggcttggg ataccccgcg acgaccacga
     2821 tcagcttgtc gcgttcgtct tccatcagct tcaggagggt cgcgatggcc tccttcccga
     2881 agtcctcggc gctgttgacc cacagggcgt acgcctcgtc gatgaacagc gtgccgccga
     2941 gcgccgactt cacgacctct tgcaccttcg gcgcggtctg cccgacgtag cccgacacga
     3001 ggccaccgcg gtccgtctcg acgaggtggc ccttctcgag cacctgcagc acgccgtaga
     3061 ccttcgagag caaccgggcc acggtcgtct tgccggtgcc cgggttgccg acgaagacga
     3121 ggtggcggct gcgctcgggg acgggcagcc cgtgttcacg gcgcagcttc tcgacccggg
     3181 tgaggttgac gatgtggtgc acttcacgct tgac
//
LOCUS       DASUGA010000818         6330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1627583, whole genome shotgun sequence.
ACCESSION   DASUGA010000818 DASUGA010000000
VERSION     DASUGA010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627583"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFX21_16100"
     CDS             <1..262
                     /locus_tag="VFX21_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017276034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5097548.1"
                     /translation="LGLAYRCVEEGGSLRDEAEALATRLAAGPTRSLGLSKRLLNTTF
                     ETDLPTALELEAAFQALATTSPDVVEGMTAFREKRDARFTGT"
     gene            259..690
                     /locus_tag="VFX21_16105"
     CDS             259..690
                     /locus_tag="VFX21_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014180538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5097549.1"
                     /translation="MTTAVQRVAAYAVCVDDANRLLLCRLSDVTDVPGMWTLPGGGID
                     FGEHPEDAARREVFEETGYEARIEDVVAIDSARRSFVDNDTEFDYHSVRIVYRAEVAS
                     GSLTYEVGGSTDMAQWFSIDEAASVPLSDVARIGTRLAWGA"
     gene            710..1627
                     /locus_tag="VFX21_16110"
     CDS             710..1627
                     /locus_tag="VFX21_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005083569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5097550.1"
                     /translation="MGLFDGKVAIVTGAGRGIGRSHALLLASEGAAVLVNDLGGSAGG
                     DGADATPAQQVVDEITAQGGKAAANYDSVSSWSGAEQMIAQAVDAFGGLDVLINNAGI
                     LRDKMSFNMDEAEWDAVIDVHLKGHFAPSRFAAAYWRAKAKETGSAVNAKIVNTASES
                     GLYGNAGQANYAAAKAGIASMTIVMARELERSGVRVNAICPVARTRLTEQVAGDFMNP
                     KEGEFDRFAPENISAVAGWLASDLSAGVTGQVLKVIGGQVQLLRGWRPITESTDDKPW
                     TITGIDGIADALFEGNDRGTPPFMPEVSS"
     gene            1638..2915
                     /locus_tag="VFX21_16115"
     CDS             1638..2915
                     /locus_tag="VFX21_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5097551.1"
                     /translation="MTAILPTTSLRLDEIELGNWELWQRPDREGIFAKLRADAPVSFH
                     EEPVPPPDIPYPQGPGFWAVTRFADVMQVSRDPDTFRSWPGTNIGDIPPEISEWLGSM
                     INMDAPKHTKLRLIVNRGFTPRQVARIEESVHQQAREIVDRVAPLGECDFVTEIAAAL
                     PLQIICDMMGIPREDTKRIFELTNTILGVGDPEYVQTIEELMGAGMELFQYGLALAED
                     RLRNPRDDITTTLMQAEVEDENGRHRLASSELGSFFLLLVVAGNETTRNAISHGMKAL
                     TDNPEQRAILTADYAGVADTAVEEIVRWATPVIHFRRTASRDTVVGDQAIKQGDKVVM
                     FYNSANRDERAFADPYNFDVRRTPNEHVGFGGGGPHFCLGANLARREIKVMFDELLRR
                     LPDIEITGEPDMLQSNFIHGIKRMPCAWHTVRD"
     gene            complement(2949..4202)
                     /locus_tag="VFX21_16120"
     CDS             complement(2949..4202)
                     /locus_tag="VFX21_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097552.1"
                     /translation="MKVVVVGDDGRTVIEGAGDDLMAEIRVRRGILKRVTVSAVVVAS
                     LALAAFAVGVGTPQRPKPDTDINAYALLGWDSVNIKGKQAALVRGGNIGVVNKKGTAT
                     ICVNGALSMDAGSQVVADKVKTGTRCDLGDVFSGDGTPGAGTYDSWSKWNPPLALTNV
                     PAIPSIDRCDESKPITVVSQRLGGQPEGPAHEAPPATYGAFTMKDEAAYKLRAGNYVF
                     CSVTIGKGVALTIDPGTTVTVLSTFSISNNSAIKGAGVDNARWTVLGRKKMNKVTSTY
                     GKLLGPSETVVNFSKHTEIHSTFFAPNNTIGLGNDTSLFGHFWARRLISDANIRVTTP
                     TTQSTTTTTVRANTSTSTSTTSTTRPSSTSSSSSSTTTAPKGSTSTSSTTSTTRPSSS
                     TSSSTSTTTTSMPVSTTEPDTLPDP"
     gene            4307..5485
                     /locus_tag="VFX21_16125"
     CDS             4307..5485
                     /locus_tag="VFX21_16125"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5097553.1"
                     /translation="MELAWSETDEAFRAELDAFVDEHMPPEAAGGADFMGEYSGDEIV
                     PRWLREWQATLFDHGWMIPAYPPELGGRNCTPTQTLIYLETMARRRIPRSGHFPGYAI
                     VTPSLLEFGNDEQRALAPAAARGDTIWCIGMSEPNAGSDLAGLQTRADVYDDRFVVNG
                     QKVWTSYATIAQKCFCYVRTDPSAPKHKGISLLIIDMDTPGIDVRPLSHISRKADFAE
                     VFFTDVEVPRANLVGDLNRGWAITQGSLAHERAGLWVEGVARFEQTIDALIDLARRTG
                     RSEDPIIRRKIASAYEVASSLRSLGYKGFASFAQGSSAPEHSYMKMATSEAGKAAYEL
                     GMEIAGPLAPVTDDRYGVEAGRWVHHFMVSFANTIAGGSSEIQRNIIAQRVLGLPRGA
                     "
     gene            5488..>6330
                     /locus_tag="VFX21_16130"
     CDS             5488..>6330
                     /locus_tag="VFX21_16130"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5097554.1"
                     /translation="MDFTLSDDQQLLADTARRLFDKECPPALVRAHIDDPDAYAPLWA
                     HVREYAALGAGPATDCCLFLDASGHAAAPGPLLATLLYTALVDGDGSEVGTVAVVDDP
                     TMPFVLEVDRVERVAFVLPGPRVAVVEARDVNASFVETVDFSRRLFAVDGDASRVDSA
                     TGSTDALAAWRDRAHAYIAAETTGTARRIFTMALQYAKERYQFDVPIGSFQAIQHKLA
                     EMSLALERATAAVQYAAMTVDAAAPDRSRACHVAKAAAGEAARRIVKDGIQIHGGIGY
                     TWEHD"
BASE COUNT         1034 a         2193 c         2101 g         1002 t
ORIGIN      
        1 gctcgggctc gcctaccgct gcgtggagga gggaggatcg ctccgcgacg aggccgaagc
       61 gctcgccacc cgcctcgcgg ccggcccgac gcgttcgctc ggcttgtcga aacgcctcct
      121 caacacgacg ttcgaaaccg atctgccgac ggcactcgag ctcgaagctg cgttccaggc
      181 gctcgccacg acgtcgcccg acgtcgtcga aggcatgacc gcctttcgcg agaaacgcga
      241 cgcgcgcttc accggcacgt gaccacggcg gtccaacgcg tcgccgcgta cgcggtgtgc
      301 gtcgacgacg cgaaccgact gctgctctgc cggctctccg acgtcaccga cgttcccggc
      361 atgtggacgt tgcccggcgg cggcatcgac ttcggggagc acccggagga tgcggcgcgg
      421 cgcgaggtgt tcgaggagac cggctacgaa gcgcgcatcg aagatgtcgt cgcgatcgac
      481 tcggcgcggc ggagcttcgt cgacaacgac accgagttcg actaccacag tgtgcgcatc
      541 gtgtatcgcg ccgaggtcgc cagcggctcg ttgacgtacg aagtcggcgg gtcgaccgac
      601 atggcgcagt ggttctcgat cgatgaggcc gcgtcggtgc cgttgtcgga cgttgcccgc
      661 atcggcactc gtctcgcctg gggcgcataa cgcgttctag ggtgcccgca tgggtctctt
      721 cgacggcaaa gtcgcaatcg tcaccggcgc ggggcgcggc atcggccgca gtcacgcact
      781 cctgctcgcc agtgaaggcg cggcggtcct cgtcaacgac ctcggcggca gcgcgggtgg
      841 cgacggtgcc gatgccacgc ccgcgcaaca ggtggtcgac gagatcaccg cgcaaggcgg
      901 aaaggccgcc gccaactacg acagcgtgtc gtcgtggtcg ggcgccgagc agatgatcgc
      961 ccaggcggtc gacgcgttcg gtggcctcga cgtgctcatc aacaacgccg gcatcctgcg
     1021 cgacaagatg agcttcaaca tggacgaggc cgagtgggat gccgtcatcg acgtccacct
     1081 gaagggccac ttcgctccgt cgcgcttcgc ggccgcgtac tggcgggcga aggccaagga
     1141 gaccggttcg gcggtcaacg cgaagatcgt caacaccgcg tcggagtccg ggctctacgg
     1201 caacgccggc caggccaact acgcggccgc gaaggccggc atcgcgtcca tgacgatcgt
     1261 catggcacgc gagctcgaac gcagcggcgt gcgcgtcaac gcgatctgcc ccgttgcccg
     1321 cacgcgcctg accgaacagg tcgcgggcga cttcatgaac cccaaggaag gcgagttcga
     1381 ccgcttcgcc cccgagaaca tctcggccgt tgcgggctgg ctcgcatccg acctctcggc
     1441 cggcgtcacc gggcaggtgc tcaaagtcat cggcggtcag gtgcagctgc tccggggctg
     1501 gcggccgatc accgagtcca ccgacgacaa gccgtggacg atcaccggca tcgacggcat
     1561 cgccgacgcc ctcttcgagg gcaacgaccg cggcacgccg cccttcatgc ccgaggtttc
     1621 gtcgtagtct cggccgcgtg actgcgatcc tgccgacgac atcactgcgg ctcgacgaga
     1681 tcgagctggg caactgggag ctgtggcaac gccccgaccg cgaaggcatc ttcgccaagt
     1741 tgcgcgccga cgcgcccgtg agcttccacg aagagcccgt cccgccgccc gacatcccct
     1801 acccgcaggg tccgggattc tgggcggtca cccgctttgc cgacgtgatg caggtgtcgc
     1861 gcgatcccga caccttccgc tcctggccgg gcaccaacat cggcgacatc ccgcccgaga
     1921 tctccgagtg gctgggctcg atgatcaaca tggacgcgcc caagcacacg aagctgcgct
     1981 tgatcgtcaa ccggggcttc acgccgcgcc aggtcgcgcg catcgaggag tcggtacacc
     2041 agcaggcgcg cgagatcgtc gatcgcgtcg cgccgctcgg cgagtgcgac ttcgtcaccg
     2101 agatcgcggc ggcgctcccc ttgcagatca tctgcgacat gatgggcatc ccgcgcgagg
     2161 acaccaagcg catcttcgag ctgacgaaca cgattctcgg cgtcggcgac cccgagtacg
     2221 tgcagacgat cgaggaactc atgggcgcgg gcatggagct gttccaatat gggctcgcgc
     2281 tcgccgaaga ccgcctccgc aacccgcgcg acgacatcac gacgacgttg atgcaggccg
     2341 aggtcgagga cgagaacggc cgccaccgtc tcgcgtcgag cgagctgggc tcgttcttcc
     2401 tgctgctcgt cgtcgccggg aacgagacga cgcgcaacgc gatctcacac gggatgaagg
     2461 cgctgaccga caaccccgag cagcgtgcga tcctgaccgc cgactacgcc ggagtcgcag
     2521 acaccgccgt cgaggagatc gtccgctggg cgacgcccgt catccacttc cgccgcaccg
     2581 catcacgcga cacggtcgtc ggcgaccagg ccatcaagca aggcgacaag gtcgtcatgt
     2641 tctacaactc cgcgaaccgc gacgagcgcg cgttcgcgga cccgtacaac ttcgacgtcc
     2701 gtcgcacacc gaacgagcac gtcggcttcg gcggcggcgg cccccacttc tgtctcggcg
     2761 ccaacctcgc gcgccgcgag atcaaggtga tgttcgacga gctgttgcgc cggctgcccg
     2821 acatcgagat caccggcgag cccgacatgc tgcagagcaa cttcatccac ggcatcaaac
     2881 gcatgccgtg cgcgtggcac accgtccgcg actgacttcg tcgatcggct cgcctctcgt
     2941 tggccgatct acggatcggg cagcgtgtcg ggctccgtcg tcgacaccgg catcgacgtg
     3001 gtggtcgtcg acgtcgagct ggacgtcgac gacgacggtc gagtcgtaga cgtcgtcgaa
     3061 ctcgtcgatg tcgatccctt cggcgccgtg gtcgtcgacg aagaagacga cgaggtcgac
     3121 gacggccggg tggtcgaggt cgtcgaggtc gacgtcgagg tgttcgcgcg cacggtcgtc
     3181 gtggtcgtcg actgggttgt cggcgtcgtg accctgatgt tcgcgtccga gatcagacgg
     3241 cgcgcccaga agtgaccgaa cagggacgtg tcgttgccga gcccgatcgt gttgttcggg
     3301 gcgaagaacg tcgagtggat ctccgtgtgc ttcgagaagt tcacgacggt ctcgctgggc
     3361 cccaggagct tgccgtacgt cgatgtgacc ttgttcatct tcttgcgacc cagcacggtc
     3421 cagcgcgcgt tgtcgacgcc agcgcccttg atcgccgagt tgttgctgat cgagaacgtg
     3481 gagaggacgg tgacggtcgt cccggggtcg atggtcagtg cgacgccctt gccgatggtc
     3541 acgctgcaaa agacgtagtt gccggcgcgc agcttgtagg cggcttcgtc cttcatggtg
     3601 aaggcgccgt acgtcgcggg cggcgcttcg tgcgccgggc cttccggctg tccgccgagg
     3661 cgttgggaga ccacggtgat cggcttggac tcgtcgcagc ggtcgatcga cgggatggcc
     3721 gggacgttgg tgagcgcgag cggcgggttc cacttgctcc acgagtcgta cgttcccgcg
     3781 cccggtgtgc cgtcacccga gaacacgtcg ccgagatcgc aacgcgtgcc ggtcttcacc
     3841 ttgtcggcga cgacctgcga gccggcgtcc atcgacagtg cgccgttgac gcaaatagtg
     3901 gcggtcccct tcttgttgac gacgccgatg ttgccgccgc gcaccaatgc cgcctgcttg
     3961 cccttgatgt tgaccgaatc ccacccgagc agcgcgtacg cgttgatatc ggtatcgggc
     4021 ttcggccgct ggggcgtgcc gaccccgacg gcgaacgcgg cgagcgccag tgaagccacc
     4081 actaccgccg acacagtgac gcgcttcaga atcccgcgcc gcacgcgaat ctcggccatg
     4141 agatcgtccc ccgctccttc gatgaccgtg cgtccgtcgt cgccgacgac gaccactttc
     4201 accacccaac gtagtcacaa cccctgaaac ggtgcaaaac tggtcgaacc tgccatatat
     4261 ggcccggcct gacaccggtg tcaggtctgt gccaacgtgg gcggtcgtgg aactggcatg
     4321 gagcgagacc gacgaggcgt tccgcgccga gctcgacgcg ttcgtcgacg agcacatgcc
     4381 gcccgaggcc gccggcggcg ccgacttcat gggcgagtac tccggggacg agatcgtccc
     4441 gcggtggctg cgcgagtggc aggcgacgct gttcgaccac ggctggatga tcccggcgta
     4501 tccgcccgag ctcggcggcc ggaactgcac accgacgcag acgctgatct atctcgagac
     4561 gatggcgcgc cggcgcatcc cgcgctccgg tcacttcccg ggctacgcga tcgtcacgcc
     4621 gagcctcctc gagttcggca acgacgagca gcgcgcactc gcgcccgcgg cggcgcgcgg
     4681 tgacacgatc tggtgcatcg gcatgagcga gccgaacgcc ggctcggatc tcgccgggct
     4741 gcaaacccgt gccgacgtgt acgacgaccg cttcgtcgtg aacggccaga aggtgtggac
     4801 gtcgtacgcc accattgccc agaagtgctt ctgctacgtg cgcaccgatc cgtcggcgcc
     4861 gaagcacaag ggcatctcgc tgctcatcat cgacatggac acgccgggta tcgacgttcg
     4921 gccgctgtcg cacatctcgc gcaaggccga cttcgccgag gtgttcttca ccgacgtgga
     4981 agtgccgcgc gcgaatctcg tcggcgatct caaccgcggc tgggcgatca cccaggggtc
     5041 cctggcccac gagcgcgcgg gactgtgggt cgagggcgtc gctcgtttcg agcagaccat
     5101 cgacgcgctg atcgacctcg cccgccgcac cggccgcagc gaggacccga tcattcgccg
     5161 caagatcgcg tcggcctacg aggtcgcatc gagcctgcgc tcactcggct acaaggggtt
     5221 cgcgtcgttc gcgcaagggt cgtcggcgcc cgagcactcg tacatgaaga tggcgacgtc
     5281 cgaagcgggc aaggccgcgt acgagctcgg catggagatc gcgggaccgc tcgcgccggt
     5341 caccgacgat cgctacggcg tcgaagccgg tcgctgggta caccacttca tggtgtcgtt
     5401 cgccaacacg atcgcgggcg ggtcgtccga gatccagcgc aacatcatcg cgcagcgggt
     5461 cctcggattg ccgcgcggag cctgacgatg gacttcacgc tcagcgacga tcagcagctg
     5521 ttggcggaca ccgcgcgccg cctgttcgac aaggaatgtc cgcccgcgct cgtccgcgca
     5581 cacatcgacg atcccgacgc gtacgcgccg ctgtgggccc acgtacgcga gtacgcggcg
     5641 ctcggtgcgg gtccggcgac ggactgctgc ttgttcctcg acgcgtccgg ccacgcggcc
     5701 gcgcccggtc ccctcctcgc gacgttgctg tacaccgcgt tggtcgacgg tgacggcagc
     5761 gaggtgggca ccgtcgccgt cgtcgacgac cccaccatgc ccttcgtgct cgaagtcgac
     5821 cgcgtcgagc gcgtcgcgtt cgtacttccc gggccgcgcg tcgcagtcgt cgaagcgcgc
     5881 gatgtcaacg cgtcgtttgt cgagaccgtc gacttctcgc gccgcctctt cgccgtcgac
     5941 ggcgacgcct cgagggtcga ctcggcgacc ggcagtaccg acgcgctggc cgcgtggcgc
     6001 gatcgcgcgc acgcgtacat cgcggccgag acgaccggca ccgcccgccg catcttcacg
     6061 atggcgctcc agtacgcgaa ggagcgctac cagttcgacg tgcccatcgg gtcgttccag
     6121 gcgatccagc acaagctcgc ggagatgtcg ctcgcgctcg aacgggcgac cgccgcggtg
     6181 cagtacgcgg ccatgacggt cgacgccgcg gcaccggatc gttcccgcgc ctgccacgtc
     6241 gccaaagccg cggcgggcga agcggcgcgc cgcatcgtca aggacggcat ccagatccac
     6301 ggtggcatcg gctacacgtg ggagcacgac
//
LOCUS       DASUGA010000819         3387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_689722, whole genome shotgun sequence.
ACCESSION   DASUGA010000819 DASUGA010000000
VERSION     DASUGA010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689722"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2066
                     /locus_tag="VFX21_16135"
     CDS             <1..2066
                     /locus_tag="VFX21_16135"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX5097555.1"
                     /translation="IVPATCVIVAILWGVLPFVIDALLAGRAPAAITPAPITQASPPV
                     TLTTIVCIGDEPNEVARASVALATEAGPAIAVAPSELAQVIAGVETDAVLIVSASAFP
                     TRDALRVAGALHDDVGWVVGRVAAYNDDAYAPRVRALVESRRRQRARSAGLVTWEPDA
                     VIVRTALLRALPLDRAAPRGSWLRSLAARGYRGVETDVAVARRAVPTDGPSFWPSEAM
                     RRRAAAADLARATTQGSLRARVLACSGLARELYAYQLLLFLAAPVLAAFGGSFPFRCS
                     AVGFAAVLGAPAIARWACSRRAIGFFPRADLRAAIYDIPGSLLALPSALTRRVRRPRF
                     HVPEEPLLLAAIVTTVAVTLPLFDRPPTSDARIDFTVAWTIVMVAVLWTAAVRAIGPA
                     GPGRASYRLKAAGLAVVDGRDGRIVDASPGGAAIAGPFATTNVGDGVSVAVTFAESGA
                     TVAVQGTVVARRASGAEFVLGIQLADDDSMRAAWIRGVADATAPASPASARVRSARRT
                     RETVAERRSRRVLRRAQLAVVGTVSLAAVALLALVLVGYRPLVVRSGSMVPTFRVGDV
                     AIADWIEANDLRPGDVVSFRDAELGGESITHRVRAVQRRGDVLHVETRGDANDSSEYW
                     STPSDALVGRVVGRVPGAGTVLSAIGRGAVRVTLLVLAAIVTAAAVLLAIARERRTRP
                     VPTRV"
     gene            complement(2073..3254)
                     /gene="moeB"
                     /locus_tag="VFX21_16140"
     CDS             complement(2073..3254)
                     /gene="moeB"
                     /locus_tag="VFX21_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEX5097556.1"
                     /translation="MPSFRELLKQAKAEIREVDTTTAESRLGEATFLDVREQDEFEQG
                     MIPGTVFIPRGHLESQIENKITDRDAEIIIYCAAGNRSAFAAQTLEQLGYTNVASMAG
                     GFGRWKDQGRPWITPTVLEPDQRNRYHRHLLLPEVGEEGQQKLLGAKVLLLGAGGLGS
                     PAALYLAAAGVGTIGIVDMDVVDASNLQRQILHNMERIGERKVDSAKKTLTAINPDVN
                     VVTYDVRFGADNILDILDGYDVVVDGTDNFPTRYLLNDASLLKRIPVVHGSIFRFEGQ
                     VTVFDPYNGPCYRCLLPEPPPPELAPSCAEAGVLGVLPGIVGSIQALEAIKLILDLGD
                     PLRGRLLAYDALEQSFRAFKVRRDPQCPACGEHAQIVIAEYDDLCLPHAVLADGTETG
                     H"
BASE COUNT          407 a         1199 c         1232 g          549 t
ORIGIN      
        1 cgatcgtccc ggcgacgtgc gtcatcgtcg ccatcctgtg gggcgtcctg ccgttcgtca
       61 tcgacgcgct gctcgccggt cgcgcgcccg ccgcaatcac acccgctccg atcacgcagg
      121 cgtcaccgcc ggtgacactg accacgatcg tgtgcatcgg cgacgaaccg aacgaggtcg
      181 cgcgcgcgag cgtcgcactc gcgaccgagg ccggcccggc gatcgcggtc gcgccgtctg
      241 agctggcgca ggtgatcgcc ggcgtcgaga ccgacgcggt gctcatcgtc tcggccagcg
      301 cattcccgac acgggacgcg ctgcgcgtgg ccggcgcatt gcacgacgat gtcggatggg
      361 tcgtgggccg ggtcgcggcg tacaacgacg acgcgtacgc gccgcgcgtc cgcgcgctcg
      421 tcgagagccg gcgccggcaa cgcgcgcgtt cggccggcct cgtcacgtgg gagccggacg
      481 cggtcatcgt ccgcaccgcg ttgctccgcg cgttgccgtt ggaccgcgcc gcgccgcggg
      541 gctcgtggct ccggtctctc gccgcccggg gctatcgcgg cgtcgagacc gatgtggccg
      601 tggcccgccg ggccgtcccc accgacggcc cttcgttctg gccgagcgag gccatgcggc
      661 ggcgggccgc cgccgcggac ctcgcacggg ccacgaccca aggttcactt cgagcgcgcg
      721 tgctcgcgtg cagcgggctg gcgcgcgagt tgtacgcata ccagttgctg ctgttcctgg
      781 ccgcgcccgt gctcgccgcg ttcggtgggt cgttcccgtt ccgctgctcc gcggtggggt
      841 tcgccgccgt gctcggcgcg ccggcgatcg cgcgctgggc ctgctcgcgg cgcgcgattg
      901 gcttcttccc ccgcgccgac ctccgcgccg cgatctacga catccccggg tcgttgctcg
      961 cgttgccgag cgcgctgact cggcgcgtgc ggcggccgcg gttccatgtc cccgaggagc
     1021 cgttgttgct cgcggcgatc gtcaccacgg tcgcggtgac gttgccgttg ttcgaccggc
     1081 caccgacaag cgacgcgcgc atcgacttca ccgttgcctg gacgatcgtg atggtggcgg
     1141 tgctctggac ggcggcggtg cgcgcgatcg gtcccgcggg gcccggccgc gcgtcgtacc
     1201 gcctgaaggc cgcggggctc gcggtcgtcg acggccgcga cggccgcatc gtcgacgcgt
     1261 cgcccggcgg cgccgcgatc gcgggcccgt tcgccaccac caacgtcggt gatggcgtgt
     1321 cggtcgcggt caccttcgcc gagtcgggcg cgaccgtggc cgtccagggc accgtcgtgg
     1381 cccggcgagc gtcgggcgcc gagttcgtgt tgggaatcca gctggccgac gacgacagca
     1441 tgcgtgcggc ctggatccgg ggcgtcgccg atgcgactgc accggcctcg ccggcatcag
     1501 ctcgcgtgcg gagcgcacgc cgcacgcgcg aaacggtcgc cgaacgccgg agccggcgcg
     1561 tgctgcgccg ggcgcaactc gccgtcgtcg gcaccgtttc gctggccgcg gtcgcactgc
     1621 tcgcgctcgt gctcgtgggc tatcgcccgc tggtcgtgcg gtcggggtcg atggtgccga
     1681 cgttccgggt cggcgacgtc gccatcgccg actggatcga ggccaacgac ctgcgacccg
     1741 gcgacgtcgt gtccttccgc gacgccgagc tgggcggcga gagcatcacg catcgcgtgc
     1801 gcgcggtgca gcggcgcggc gacgtcctgc acgtcgagac gcgcggcgac gccaacgact
     1861 cgagcgagta ctggtcgacg ccttcggacg cgctggtcgg ccgggtcgta ggacgtgttc
     1921 ccggtgccgg caccgtgctg tcggcaatcg gtcgcggcgc ggtacgcgtc acgttgctcg
     1981 tgctcgccgc gatcgtcacc gccgcggcgg tgctgctcgc aatcgcgcgc gagcgccgga
     2041 ctcgtccggt accgactcgc gtgtgagccg cgtcagtgac cggtttcggt gccgtccgcg
     2101 agcaccgcgt gcggcaggca gaggtcgtcg tactcggcga tgacgatctg cgcgtgctcg
     2161 ccgcaggccg ggcactgcgg gtcgcgccgg accttgaacg cccggaagct ctgctccagc
     2221 gcgtcgtagg ccagcagccg gccgcgcagc gggtcgccga ggtcgaggat gagcttgatc
     2281 gcctcgagcg cctggatcga cccgacgatg ccgggcagca cgccgagcac gccggcctcc
     2341 gcgcacgacg gcgccagttc cggcggcggc ggctcgggca acaagcagcg gtagcacggc
     2401 ccgttgtagg ggtcgaacac cgtgacctgg ccctcgaagc ggaagatcga accgtgcacg
     2461 acggggatgc gcttcaacaa cgacgcgtcg ttcaagaggt agcgcgtcgg gaagttgtcg
     2521 gtgccgtcga cgacgacgtc gtagccgtcg aggatgtcga ggatgttgtc ggcgccgaag
     2581 cgcacgtcgt aggtcacgac gttcacgtcg gggttgatcg cggtcagcgt cttcttcgcg
     2641 ctgtcgacct tgcgctcacc gatgcgctcc atgttgtgga ggatctggcg ctgcaggttc
     2701 gacgcgtcga cgacgtccat gtcgacgatg ccgatcgttc cgacgcccgc ggccgcgagg
     2761 tagagcgcgg cgggggagcc gaggccaccc gcgccgagca gcagcacctt cgcgccgagc
     2821 agcttctgct ggccttcttc accgacttcg ggaagcagta ggtgccggtg gtagcggttg
     2881 cgctggtcgg gctcgagcac cgtcggcgtg atccacggcc ggccttggtc cttccaacga
     2941 ccgaagccgc cggccatcga cgcgacgttc gtgtagccga gctgttcgag ggtctgcgcc
     3001 gcgaacgccg aacggttgcc ggccgcgcag tagatgatga tctcggcgtc gcggtcggtg
     3061 atcttgttct cgatctggct ctcgaggtgg ccgcgcggga tgaagacggt gcccgggatc
     3121 atcccctgct cgaattcgtc ctgctcacgt acgtcgagga aggtcgcttc gccgaggcgt
     3181 gactcggcgg tcgtggtgtc gacttcccgg atctcggcct tggcctgctt cagcagctcg
     3241 cggaagctgg gcatcgttcc tccaattgcg gacccattcg ggacctaaat cctaccgcgt
     3301 cggtcgggaa ttcccaacgg gccgccaccg gcggttgttc ccaaccgcgg tgcatacgcg
     3361 accgccccgt actcggccgt cgccgcc
//
LOCUS       DASUGA010000820         2911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1801962, whole genome shotgun sequence.
ACCESSION   DASUGA010000820 DASUGA010000000
VERSION     DASUGA010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1801962"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VFX21_16145"
     CDS             complement(<1..261)
                     /locus_tag="VFX21_16145"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5097557.1"
                     /translation="MLTVDFDRLGLVRGERLLDLGAGGGRHAFEAMRRGARVTALDYS
                     AADLKDAVAVAGAMLAGNEISVDVWAGAVNGDALDLPFPDGAF"
     gene            complement(422..1729)
                     /locus_tag="VFX21_16150"
     CDS             complement(422..1729)
                     /locus_tag="VFX21_16150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009996064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5097558.1"
                     /translation="MASLRPHVLRPGDPLRVAFFVYRGNPHCGGQGVYSRHLTRELTE
                     LGHEVTLFAGEPYPVVDDPVTFDPVPSLDLYKRENPFRVPWPYEFRTATDLQEFAIMC
                     TAGYPEPYAFSKRVYQRLRDRRHEFDLIHDNQCLGSGLLGFVNDGWPFVHTLHHPITV
                     DRDLDLAHATSLHRKLTLRRWYGFLNMQMRVARQLPRHLTVSENSKKDIVAQMGVDID
                     TLHIVPVGVDQKQFRPMPHVQRVPGRLMTTASADVPLKGLTYLIEALAKVRTEREDAH
                     LVVIGRPRHKSAVPAQIERLGLQGAIEFVSGVSDERIVELYAEAEIAVVPSLYEGFSL
                     PSIEAMACGVPIVATTGGALPEVVGRNGESGVLVPPADPGALAATIVDVLNNPELRSK
                     LADGGRSRVLDRFTWRRTAEGTAEHYYLELEAHARRRHDAGIR"
     gene            1867..>2911
                     /locus_tag="VFX21_16155"
     CDS             1867..>2911
                     /locus_tag="VFX21_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097559.1"
                     /translation="MAGITPALISRSGGVADPRWSPSGARLAWVTSFDGRSDLVVAPA
                     DGSGPPVVVTAESGAGGGYAWVTDDELVVGAGDGRLVIVGADGGEIRALTRDGRAFAP
                     AVSTRGEVACSLERDDACDIVVVPLDGSAWPVRVSNADYAWDCSWSPDGTSLAWHEWD
                     LPDMSWDSSRIVVRHGNAAKVIAGGEAIGVGQPRFSPTGERIAFVSDAEGWSVLWTAD
                     LDGGNARPVLRETFEHAEPAWGAGQRSYAWSPDGTQLAWCRNENGFGRLVIASLDARS
                     ARELSKGWHRGLDWSEHGLACIRSGAATPPQVVVLAANGSGRRAIARGPVGGFEATGL
                     VEPRAVTWKSGSAT"
BASE COUNT          417 a          980 c         1088 g          426 t
ORIGIN      
        1 gaacgctccg tccgggaacg gcaagtccaa cgcgtcgccg ttcaccgcac cggcccacac
       61 gtcgacgctg atctcgttgc cggcgagcat cgcgccggcg accgcgactg cgtccttcaa
      121 gtcggcggcc gagtagtcga gcgcggtgac gcgcgcgccg cggcgcatcg cttcgaatgc
      181 gtgccgcccg ccgcccgcgc cgaggtcgag cagtcgttcg ccgcgcacca gcccgaggcg
      241 atcgaagtcg actgtgagca tcagtgcacc gtccggcaac gttcgccgcg ctcacgccgg
      301 ccagtgacgc cgctcgcggc gtcctcctcc tcaaaagagg cccactcttc cttcgtcgtg
      361 cgtccttgcg agcgacgccc ctgactcgcc gtgaccatca acaacgtcgc cggacggagc
      421 actagcggat cccggcgtcg tggcggcgcc gcgcgtgcgc ctcgagctcg aggtagtagt
      481 gctcggccgt gccttcggcc gtgcggcgcc aggtgaagcg gtcgagcacg cggctgcgcc
      541 cgccgtcggc cagcttggac cgcagttcgg ggttgttcag cacgtcgacg atggtcgcgg
      601 ccagcgcgcc ggggtcggcg ggcggtacca gcacgccgct ctccccgttg cggccgacga
      661 cctcgggcag tgcgccgccg gtcgtggcga cgatcgggac gccgcaggcc atcgcttcga
      721 tcgagggcag cgagaagcct tcgtacaacg acggcacgac cgcgatctcg gcctcggcgt
      781 acagctcgac gatgcgctcg tcgctgacac cgctgacgaa ctcgatcgcg ccctgcagcc
      841 cgagtcgttc gatctgcgcg ggcaccgcgc tcttgtgacg cgggcggccg atgacgacga
      901 gatgcgcgtc ttcgcgttcg gtgcgcacct tcgcgagcgc ctcgatgaga tacgtgaggc
      961 ccttcagcgg cacgtccgcg ctcgcggtcg tcatgagacg acccggaacg cgctgtacgt
     1021 gcggcatcgg gcggaactgc ttctggtcga cgccgaccgg cacgatgtgc aacgtgtcga
     1081 tgtcgacacc catctgggcg acgatgtcct tcttcgagtt ctcggacacc gtgaggtgtc
     1141 gcggcagctg acgcgcgacg cgcatctgca tgttcaagaa cccgtaccaa cgccgcaacg
     1201 tgagcttgcg gtgcagcgac gtcgcgtggg ccaggtcgag gtcgcggtcg acggtgatgg
     1261 ggtggtgcag cgtgtgcaca aacggccagc cgtcgttcac gaaaccgagc aaccccgaac
     1321 cgaggcactg gttgtcgtgg atcaggtcga actcgtggcg ccggtcacgc agccgctggt
     1381 acacacgctt ggagaacgcg tacggctcgg ggtagccggc cgtgcacatg atcgcgaact
     1441 cctggagatc ggtggccgtg cggaactcgt acggccacgg cacccggaag gggttctcgc
     1501 gcttgtagag gtcgagactg ggcaccgggt cgaaggtgac cgggtcgtcg acgaccgggt
     1561 acggctcgcc cgcgaagagg gtgacctcgt ggcccagctc ggtcagctcg cgcgtgaggt
     1621 ggcggctgta gacaccttgg ccgccgcagt gcgggttgcc gcggtagacg aagaacgcga
     1681 cacgaagcgg gtcgccgggg cgcagcacgt gcggacgcag tgaagccacg gacggcgcag
     1741 cgagggagcc attggggctg gaagcagaca tgaagaacca aggatggagt gccgccgcgc
     1801 aatggggcaa gccgggtggt cgggatcgcg cgctgagcgc ggcggtcggc gcactacgtt
     1861 tcggccgtgg ccggcatcac tcccgcgctg atctcccggt ccggcggcgt cgccgacccg
     1921 cggtggagcc cgtcgggggc ccgtctggcc tgggtcacga gcttcgacgg ccggagcgac
     1981 ctggtcgtgg cgccggccga cgggagtggc ccgccggtcg tggtgaccgc cgagtcgggc
     2041 gcgggcggcg ggtacgcctg ggtgaccgac gacgagctcg tggtcggtgc gggcgacggg
     2101 cgcttggtca tcgtcggcgc cgacggcggc gagatccggg ccctcacgcg cgacgggcga
     2161 gcattcgcgc ccgcggtctc gacgcgtggc gaggtcgcct gctcgctcga acgcgacgac
     2221 gcgtgcgaca tcgtcgtcgt gccgctcgac ggcagcgcgt ggccggtgcg ggtgtcgaac
     2281 gccgactacg catgggactg ttcgtggtcg cccgacggca cgtcgctcgc gtggcacgag
     2341 tgggacctcc ccgacatgtc gtgggactcg tcgcgcatcg tcgtgcgcca cggcaacgcg
     2401 gccaaggtga tcgccggcgg cgaagccatc ggcgtggggc aaccgcgctt ctcgcccacc
     2461 ggcgagcgca tcgcgttcgt gagcgacgcc gaaggatggt cggtgctgtg gaccgccgat
     2521 ctcgacggcg gcaacgcacg gccggtgctg cgcgagacgt tcgagcacgc ggagccggcg
     2581 tggggcgcgg gacagcgatc gtacgcctgg tcgcccgacg gtacgcagct cgcgtggtgc
     2641 cgcaacgaga acggattcgg ccggctcgtg atcgcgtcgc tcgacgcgcg ttcggcgcgc
     2701 gagctctcga agggctggca ccgcggactc gactggagtg agcacggcct cgcgtgcatc
     2761 cggtcgggcg ccgcgacacc gccgcaggtc gtcgtgctcg ccgcgaacgg ctccggacgg
     2821 cgcgcgatcg cgcgcggtcc cgttggcggc ttcgaggcga cgggcctggt cgagccgcgc
     2881 gcagtgacgt ggaagtcggg gagtgcgacc g
//
LOCUS       DASUGA010000821         2337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2043535, whole genome shotgun sequence.
ACCESSION   DASUGA010000821 DASUGA010000000
VERSION     DASUGA010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2043535"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="VFX21_16160"
     CDS             <1..1061
                     /locus_tag="VFX21_16160"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX5097560.1"
                     /translation="TPDEELVRRAGVSMEAVQLLRSSEVVDLHLESYIPPRIWGFDLA
                     VRHDPPPLRGYFFGHLDFPRALDGGLTGAMWSIATNILRTKRGRLATLKKNVAGLAAA
                     FDRTGGAIRPVRTHAEYVAARREGAHAALIAVQGGNALEAAGDAVADAVPDRLLTRVT
                     LVHLSNSCYGGTSSPANVLTGERGLTNAGRELVRQLDAARIYVDLAHVSKRGFWDAVE
                     VHDKGLPLLVTHTGVDGVKPMWRNVDDAQIRAVADTGGVIGIIVAENFLRPRGGPRDG
                     RLLVDHMAHAIDAGGEDCVAIGTDYDGFIHPPKDLRDGALGYVRLVQHMLDRGFTDTR
                     IRKILGDNFLRTFRRMRP"
     gene            complement(1172..>2337)
                     /locus_tag="VFX21_16165"
     CDS             complement(1172..>2337)
                     /locus_tag="VFX21_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097561.1"
                     /translation="CLAHLRTAICATHLDVAMLTDHAPHMNEGTIEDALLAAADDELI
                     RDGAGNAYASIVTCEDGHEVVLTVGSENALMPVGLRRHVLEGVTVDELESAYDAETPA
                     AVDEFRAAGALVLVNHTEQRPLDSLRMLAPDGIEIYNIHANLDPRIRSEYLGLDSDFL
                     ADLIRLIMPGNGVAPDLLALAFLEENRNDLDKWDALLAEGVRMPGLAGSDAHENAFAN
                     PLADGERADSYRRVMRWFSNHMLVDTTLPRPERYEEALAAARFYVAFEAFGSPVGFDF
                     IAETATVTAEMGETVAPGATLRVVAPTLPPDQPRDPAPDIRLRILRSTATGAVEVAAG
                     TGPTLEHVATEPGAYRAEVFILPNHARPYVGARSDLIREHVWIYSNAIHVVPD"
BASE COUNT          343 a          781 c          854 g          359 t
ORIGIN      
        1 gaactcccga cgaagagctc gtgcggcgcg ctggcgtgtc gatggaggcg gtgcagctcc
       61 tgcgcagctc ggaggtcgtc gacctccatc tcgagtcgta catcccgccg cgcatctggg
      121 gcttcgacct ggcagtgcgt cacgacccac cgccgctgcg cgggtacttc ttcgggcatc
      181 tggactttcc gcgcgcgctc gacggagggc tgacgggcgc gatgtggagc atcgcgacga
      241 acattctgcg gacgaagcgt ggtcggctcg cgacgctgaa gaagaacgtg gcgggcctcg
      301 cggccgcgtt cgatcggacg ggcggcgcga ttcgacccgt gcggacgcac gcggagtacg
      361 tcgcggcgcg cagagagggc gcgcacgcgg cgctgatcgc ggtgcagggc ggcaacgcgc
      421 tcgaggcggc gggcgatgcg gtggcggatg cggtgcctga ccggttgctc acgcgcgtca
      481 cactcgtgca cctgagcaac tcctgttacg gcgggacgtc gagcccggcg aacgtgctga
      541 ccggcgagcg cgggctgacg aacgccgggc gcgagctcgt gcggcagctc gacgcggcgc
      601 ggatctacgt cgacctcgcg cacgtctcga agcgcgggtt ctgggacgcg gtcgaagtgc
      661 acgacaaggg cttaccgctc ctcgtgacgc acaccggcgt cgacggcgtg aagcccatgt
      721 ggcgcaacgt cgacgacgca cagatccgcg cggtcgccga caccggtggc gtcatcggca
      781 tcatcgtcgc cgagaacttc ctccgtcccc gcggcggccc gcgcgacggt cgcctcctcg
      841 tcgatcacat ggcccacgcc atcgacgccg gcggcgaaga ctgcgtagcc atcggcaccg
      901 actacgacgg cttcatccac ccccccaaag acctccgcga cggcgcccta ggctacgtcc
      961 gcctcgtcca acacatgctc gaccgaggct tcaccgacac ccgcatccgc aagatcctcg
     1021 gcgacaactt cctccgcacc ttccgccgca tgcgccccta ggggacgttg ctcaagcagc
     1081 aagttgcgac gcgtaaccgc gtgaaatcac gcacccgaga tccggggacg ttgctcaagc
     1141 aacaagttgc gccaccgcgc gacgcgcttc ctcagtccgg gacgacgtgg atggcgttcg
     1201 agtagatcca gacgtgctcg cggatgaggt cgctgcgcgc accgacgtag gggcgggcgt
     1261 ggttcgggag gatgaacact tcggcgcggt aggcgccggg ctcggtggcg acgtgctcga
     1321 gcgtggggcc ggttcccgcg gcgacttcga cggcgccggt cgccgtggag cggaggatac
     1381 ggaggcggat gtcgggggcg gggtcgcgcg gttggtcggg gggcagtgtg ggcgcgacga
     1441 cgcggagtgt ggcgccgggg gcgacggtct cacccatctc ggcggtgacc gtcgcggtct
     1501 cggcgatgaa gtcgaagccg acggggctcc cgaacgcctc gaacgcgacg tagaagcgcg
     1561 ccgctgcgag cgcttcttcg tagcgctccg gtcggggcag cgtcgtgtcg acgagcatgt
     1621 ggttcgagaa ccagcgcatc acgcgccggt acgaatccgc gcgctcgccg tcggcgagcg
     1681 ggttggcgaa cgcgttctcg tgcgcgtcgc tgccggcgag gcccggcatg cgcacgccct
     1741 cggcgagcag cgcgtcccac ttgtcgaggt cgttccggtt ctcttcgagg aacgcgagcg
     1801 cgagcaggtc gggcgcgacg ccgttgcccg gcatgatcag ccggatcaga tccgcgagga
     1861 aatcgctgtc gaggccgagg tactcggagc gaatgcgcgg atcgaggttc gcgtggatgt
     1921 tgtagatctc gatcccatcg ggcgcgagca tgcgcaggct gtcgagcggt cgctgctcgg
     1981 tgtggttgac gagcacgagc gcgcctgccg cgcggaactc gtcgaccgcg gcgggcgtct
     2041 cggcgtcgta cgcgctctcc agctcgtcga cggtcacacc ctcgagcacg tggcgccgca
     2101 gcccgacggg catgagcgcg ttctcgctcc cgacggtgag cacgacctcg tggccgtcct
     2161 cgcacgtcac gatgctcgcg taggcgtttc cggcgccgtc gcggatgagc tcgtcgtcgg
     2221 ccgcggcgag cagcgcgtcc tcgatcgtgc cttcgttcat gtgcggcgcg tggtcggtca
     2281 gcatcgccac gtcgaggtgc gtcgcacaga tcgcggtgcg gaggtgcgcg aggcact
//
LOCUS       DASUGA010000822         5812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1694130, whole genome shotgun sequence.
ACCESSION   DASUGA010000822 DASUGA010000000
VERSION     DASUGA010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694130"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VFX21_16170"
     CDS             complement(<1..263)
                     /locus_tag="VFX21_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEX5097562.1"
                     /translation="MTVTPSLDEFVALARDYTVVPVWREVLADLETPLSAFAKLVGER
                     EGFLLESVEHAERWGRFSFLGRDPALTLVARGHDIEWIGGPPP"
     gene            complement(296..640)
                     /gene="hisI"
                     /locus_tag="VFX21_16175"
                     /pseudo
     CDS             complement(296..640)
                     /gene="hisI"
                     /locus_tag="VFX21_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011431829.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
     gene            703..2805
                     /locus_tag="VFX21_16180"
     CDS             703..2805
                     /locus_tag="VFX21_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018789107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX5097563.1"
                     /translation="MSQLSPPVAARRPHVLTEHGDERVDDWYWLRDRDDPEVLAHLKA
                     ENAYTDALLAPTKALQDRIYAEIKARIEETDTSAPVPDGPWEYYARTVEGQQYPIHCR
                     RPRGGRAETVLLDENVESEGRDYFSLGGFAVSPDHAVLAYAVDFDGGERYTLRFRDPQ
                     GLAPYADVVENVSYGQAWADDNRTLFYVRPDDAMRPWQVWQHTVGTGAGDDVLVFQED
                     DERFFVGVSRTRSGRFILIDSSSKTTSEVRFIPTEAPLDAPRVIAPREAGHEYAVEHH
                     WNEERGDRFLIVTNANGNARNFKLVAAPAVDPERQYWTDIVPHREDTRLEDVDAFEEH
                     IVLTERRDGLTRLNVMHVESGDMHEVAFPDPVYSAWTGHNAEFESTVLRYGYTSLVAP
                     TTDVDYEMNDRAATTVKVQPVRGYDAPQYTSERAWVTATDGTIVPLSIVRRVDTPLDG
                     SAPALLYGYGAYEYSTEAVFRAARLSLLDRGFVFALAHVRGGGELGRPWYEGGRLEHK
                     RNTFTDFIACGQFLVDNNYTSPSRLVARGGSAGGLLMGAVANLRPDLFAGIVAEVPFV
                     DVLTTMLDPSLPLTITEWEEWGDPREADAYAWMKAYSPYDNVAEHPYPAMFVTSGLND
                     PRVQYWEPTKWVQKLRACTTSGQPIALRTELGAGHGGPSGRYDAWRDEAMVLAFVCKT
                     VGLAETDVMEPERPPGRH"
     gene            2809..5220
                     /locus_tag="VFX21_16185"
     CDS             2809..5220
                     /locus_tag="VFX21_16185"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEX5097564.1"
                     /translation="MTATVGVAERAPGAEASPAEGGAGEEGVHPAPPGRRVSFSWTVL
                     ALLGTLVIVTIPFWEVLQGKWTTVSFDVNDYYAPAGQGVWRLLRAGHTPWWTPNVYAG
                     SSTLGVGQYGVFYPFNALYGYLDAATAHRWWMLSHLWLAATGMFCWSAWRWQSRFAGV
                     VSGCAYALSGFYILHLQFNPFIATATWLPWVFLGIDLVRDRWTTPRATLVAVPLALLA
                     CAGHPQMLFYAGIGVGTYLAVPLLARRPDLRGCARGAGACALGLALAAMQLLPLWRYG
                     HISIRSHFDQQQAFDRSASLRHLLSYPFPFLYGGSGRDVFNAPWTGGSQQMEVGLFAG
                     VTVLALAGCALVARRRERTVVALGAVALAGTLVMLGPNTPAGSFIYRVVPMAASFRAW
                     SRAALLPTLALAMLAGGGVQYALKNARKVQPWFAAWAATLALAALALPRAASLHPYLS
                     TGALSIAARAVPVGAAFAFVAAFGLLDHYRRAGTVAVAAVCTFELVFFATLGPWHAWS
                     MDATTARVALDTHTEGPFGAPLDAPDGIDRWFASGYGYRMMSLTKNYYGINAFDPLAP
                     KDFTDSIGGMTYDGFVTSDQFWRDTWLADILRVTTMILDTNFVPQSPQWRLTGKLPDR
                     HERVWNRTPRLPDAYLMHDVRVESLADIRSHILDKNDDLTAHAYIEKPVDGMESGTNR
                     GFTGRVTSNDVLGSGRVTVEADAPSLLVLSHAYQQGWKATVDGRSAPVRRVNALVLGV
                     PVPAGHHDVKVWFEPPGLRPGIGLAAVAGFTLFIVSPAWMFWRRRHPALVEAPETPDS
                     VAGSD"
     gene            5213..>5812
                     /locus_tag="VFX21_16190"
     CDS             5213..>5812
                     /locus_tag="VFX21_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097565.1"
                     /translation="MTEPRVLHGADGVVCEAEFRAADEPRAVVVLCHPHPQFGGSMRS
                     VVISPLFDALPALGYSCLRFNFRGVERSTGVYGEGLTEPIDVVAALDDAAPDAVGLPL
                     VLVGFSFGADMALSVADARVTAWVGIAPPLRFASAFAIAHDPRRKHLILAAHDEFRAA
                     EAVAREVASWDNTTTVVVPGASHFFVGRTDRVIDEVDALL"
BASE COUNT          850 a         2061 c         1973 g          928 t
ORIGIN      
        1 tccggcggcg ggccgccgat ccattcgatg tcgtgcccgc gcgccacgag cgtcagcgcg
       61 gggtcgcgac cgaggaacga gaaccggccc caccgctcgg cgtgctccac cgactcgagc
      121 aggaatccct cacgctcacc gacgagcttg gcgaacgccg agagcggcgt ctcgaggtcg
      181 gcgagcacct cgcgccacac gggcacgacc gtgtagtcgc gcgcgagcgc aacgaactcg
      241 tcgagcgagg gcgtgacggt cacatcttgc cgggggttgc tccgctgccg aaggcgcgga
      301 agaagcagct gcgctcgccc gtgtggcagg caccgcgccc ctcttgctcg acgacgaaca
      361 gcaacacgtc catgtcgcag tcgtacatgg cctcacgcac gaactggcgg tcgccggagg
      421 tctcgccctt gcaccagtac tcctggcggc tgcggctcca gaaccacgtg cgcccggtct
      481 cgagcgtgcg ctcgagcgac tcccggttca tccaggcgaa catgagcacc tgcttggtgc
      541 cggcctcctg gacgatcgcc gggacgaggc cttcggcgtt gaaggcgacc gcggcgagct
      601 cggcctcggt atagggaatg ggcgtggtcg tcggcgccat ggtccgccga tcgtagcggc
      661 cggcctttgt cccctctcgg gcgtttctag acttcgccgc tgatgtccca actgtcaccg
      721 cccgtcgccg cgcgccggcc ccacgtcctc accgagcacg gcgacgagcg cgtcgacgat
      781 tggtattggc tgcgggaccg cgacgacccc gaggtgctgg cacacctgaa ggccgagaac
      841 gcgtacaccg atgcgttgct cgcgccgacc aaggcgctcc aagaccgcat ctacgcggag
      901 atcaaggcgc gcatcgaaga gacggacact tccgcgccgg tgcccgacgg gccgtgggag
      961 tactacgcgc gcacggtcga ggggcagcag tatccgatcc actgccggcg accgcgcggc
     1021 ggacgggccg agacggtgct gctcgacgag aacgtcgaga gcgaggggcg cgactacttc
     1081 tcgctcggcg ggttcgcggt gtcgcccgat cacgcggtgc tcgcgtacgc ggtcgacttc
     1141 gacggcggcg agcggtacac gctgcgcttc cgcgacccgc aagggctcgc gccgtacgcc
     1201 gatgtcgtcg agaacgtctc gtacgggcag gcgtgggccg acgacaaccg cacgctgttc
     1261 tacgtgcggc ccgacgacgc gatgcggccg tggcaggtct ggcagcacac cgtcggcacc
     1321 ggcgccggcg acgacgtcct cgtgttccaa gaggacgacg agcggttctt cgtcggcgtg
     1381 tcccgcacgc gcagtggccg gttcatcctg atcgacagct cgtcgaagac gacctcggaa
     1441 gtgcgcttca tcccgacgga ggcgccgctc gatgccccgc gagtgatcgc gccgcgcgag
     1501 gccggccacg agtacgcggt cgagcaccac tggaacgagg agcgcggcga ccgcttcctc
     1561 atcgtgacga acgcgaacgg caacgcccgc aacttcaagc tggttgccgc gcccgcggtc
     1621 gatccggagc ggcagtactg gaccgacatc gtcccccatc gcgaggacac gcgtctcgaa
     1681 gacgtcgacg cgttcgagga acacatcgtc ctcaccgagc ggcgcgacgg cctgacgcga
     1741 ctgaacgtca tgcacgtcga gtcgggcgac atgcacgaag tcgcgtttcc cgacccggtc
     1801 tacagcgcgt ggaccggtca caacgccgag ttcgagtcga ccgtgctgcg ctacggctac
     1861 acgtcgttgg tcgcgccgac aaccgacgtc gactacgaga tgaacgaccg cgccgcgaca
     1921 accgtgaagg tgcagccggt ccgcggctac gacgcgccgc agtacacgtc ggagcgggcg
     1981 tgggtgaccg caaccgacgg caccatcgtc ccgctgtcga tcgtgcgccg tgtcgacacg
     2041 ccgctcgacg gctccgcacc ggcgctgctc tacggctacg gcgcctacga gtactcgacc
     2101 gaggccgtgt tccgcgccgc gcgcctgtcg ctgctcgacc gcgggttcgt attcgcgctc
     2161 gcacacgtgc gcggtggcgg cgagctcggc cggccctggt acgagggcgg acggctggag
     2221 cacaagcgca acaccttcac cgacttcatc gcctgcgggc agttcctcgt cgacaacaac
     2281 tacacgtcac cgtcgcggct cgtcgcccgg ggcggctcgg ccggcggcct gctcatgggc
     2341 gcggtcgcca acctgcgccc cgatctgttc gcgggcatcg tggccgaggt gccgttcgtc
     2401 gacgtgctga cgacgatgct cgacccgtcg ctgccgctca cgatcaccga gtgggaagag
     2461 tggggtgacc cgcgtgaagc cgacgcgtac gcgtggatga aggcctactc gccctatgac
     2521 aacgttgccg agcacccgta tcccgcgatg ttcgtgacct ccgggttgaa cgatccgcgc
     2581 gtccagtact gggaaccgac gaagtgggtg cagaagctgc gggcgtgcac cacgagcggg
     2641 caacccatcg cactgcgcac cgagctcggc gcgggtcacg gcggcccgag tggccgctac
     2701 gacgcctggc gcgacgaagc gatggtgctc gcgttcgtgt gcaagaccgt cggcctcgcc
     2761 gagacggacg tcatggaacc ggagaggccg ccggggcgtc actgaaccat gactgcgact
     2821 gtgggggtcg ccgagcgagc accgggcgcc gaagcgagcc cggccgaggg cggcgcagga
     2881 gaggaagggg ttcacccagc tccgccgggc cggcgcgtga gcttcagctg gaccgtcctc
     2941 gccctcctcg gcacgctcgt catcgtcacc atcccgttct gggaagtgct ccagggcaag
     3001 tggacgacgg tcagcttcga cgtgaacgac tactacgccc cggccggcca gggcgtttgg
     3061 cggctgctcc gcgcgggcca cacgccctgg tggacaccga acgtctatgc cggaagctcg
     3121 accctcggcg tcgggcagta cggcgtcttc tacccgttca acgcgctcta tggatacctc
     3181 gacgccgcga ccgcgcatcg gtggtggatg ttgtcgcacc tgtggctggc ggcaaccggc
     3241 atgttctgct ggtcggcatg gcggtggcag tcacgcttcg caggtgtcgt gtccggatgc
     3301 gcgtacgccc tgagcggctt ctacatcctg cacctccagt tcaacccctt catcgcgacg
     3361 gcgacgtggc tgccgtgggt cttcctcggg atcgatctgg tacgcgaccg ctggacgaca
     3421 ccgcgcgcca cgctcgtggc cgtgccgctc gcgctcctcg cctgcgccgg gcacccgcaa
     3481 atgctgttct acgcgggtat aggcgtcggg acgtatctcg ccgtcccgct gctcgcgcgc
     3541 cgccccgacc tccgcggttg cgcgcgcggc gcgggcgcct gcgcgctcgg gctcgccctc
     3601 gccgcgatgc aactgttgcc gctgtggcgc tacggccaca tcagcatccg ttcacacttc
     3661 gaccaacagc aggccttcga ccgctccgcc tcgctgcggc acctgctgtc gtacccgttc
     3721 ccgttcctgt acggcgggag cggtcgcgac gtcttcaacg cgccgtggac cggcgggtcg
     3781 caacagatgg aggtcggcct cttcgccggc gtgaccgtgc tggccctcgc cggctgcgca
     3841 ctcgtcgccc gccgccgcga gcggaccgtc gttgcgctcg gcgccgtcgc gctcgcgggc
     3901 acgctcgtga tgctcgggcc gaacacgccg gccgggtcgt tcatctaccg cgtcgtgccg
     3961 atggcggcgt cgttccgcgc gtggagccgt gccgcgttgt tgccgacgct cgcactcgcg
     4021 atgctcgcgg gcggcggtgt gcagtatgcg ttgaagaacg cgcgcaaagt ccagccgtgg
     4081 ttcgcggcct gggccgcgac gctggcactc gccgccctcg cgctcccgcg tgccgcgtcc
     4141 ctgcacccct acctcagcac cggagcgttg agcatcgcgg cgcgcgcggt tccggtcggc
     4201 gccgcgttcg cgttcgtcgc cgcgttcgga ctgctcgacc actaccgacg agccggcacc
     4261 gttgccgtcg ccgccgtctg cacgttcgag ctcgtcttct tcgccacgct cgggccttgg
     4321 cacgcgtgga gcatggatgc gacgacagcg cgcgtcgcgc tcgacacaca caccgaggga
     4381 cccttcggtg cgccgctgga cgcgcccgac ggcatcgacc gttggttcgc ctccggctac
     4441 ggctaccgga tgatgtcgtt gacaaagaac tactacggca tcaacgcgtt cgatccgctc
     4501 gcgcccaagg acttcactga ctcgatcggc gggatgacct acgacggctt cgtgacgagc
     4561 gaccagttct ggcgcgacac gtggctcgcc gacatcttgc gggtcacgac gatgatcctc
     4621 gacacgaact tcgttccgca gagcccgcag tggcggctca cgggcaagct tcccgaccgc
     4681 catgaacggg tgtggaatcg cacaccccgc ctgcccgacg cgtacttgat gcacgacgtg
     4741 cgggttgagt cgctggccga tattcgctcg cacatcctcg acaagaacga cgatctcacc
     4801 gcgcacgcgt acatcgagaa gccggtcgac ggcatggagt cggggacgaa ccgcggcttc
     4861 acgggtcggg tcacgtcgaa cgatgtcctc ggctccggtc gcgtcaccgt ggaagccgac
     4921 gcaccatcac tgctcgtgtt gtcgcacgcg taccagcagg gatggaaggc gacggtcgac
     4981 ggtcgctcgg cgccggtgcg acgtgtcaac gcgctcgtac tcggcgtgcc cgtgccagcc
     5041 ggccatcacg acgtgaaggt gtggttcgag ccgccgggtc tgcgtccggg catcgggctc
     5101 gcggccgtcg cgggcttcac gctgttcatc gtgtcgccgg cgtggatgtt ctggcgccgt
     5161 cgccaccccg cccttgtcga ggcacccgaa acgcccgata gcgtcgccgg gagtgactga
     5221 gccgagggtc ttgcacggcg ccgacggcgt cgtatgcgaa gcggagttcc gcgccgccga
     5281 cgagccgcgt gcggtcgtcg tgctgtgcca cccgcacccg cagttcggcg ggtcgatgcg
     5341 cagcgtcgtg atctcgccgt tgttcgacgc cttacccgcg ctcgggtaca gctgcctgcg
     5401 gttcaacttc cgcggcgtgg agcgcagcac cggcgtctac ggcgaaggcc tgaccgagcc
     5461 gatcgacgtc gtcgccgcgc tcgacgacgc ggcgccggac gccgtcggtc tcccgctcgt
     5521 gctggtcggc ttctcgttcg gcgcggacat ggcgctctcg gttgccgacg cgcgcgtcac
     5581 cgcctgggtc gggatcgcgc cgccgttacg gttcgcgtcc gcgttcgcga tcgcgcatga
     5641 tccgcggcgc aagcacctga tcctcgccgc gcacgacgag ttccgcgcag ccgaagcagt
     5701 ggcgcgcgaa gtggcgagtt gggacaacac cacgaccgtg gtggtccccg gcgcgagtca
     5761 cttcttcgtt ggccgcactg atcgtgtgat cgacgaagtc gacgcgctct tg
//
LOCUS       DASUGA010000823         3092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_968998, whole genome shotgun sequence.
ACCESSION   DASUGA010000823 DASUGA010000000
VERSION     DASUGA010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968998"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1400
                     /locus_tag="VFX21_16195"
     CDS             <1..1400
                     /locus_tag="VFX21_16195"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX5097566.1"
                     /translation="QRHVDPCARDVTATPLHARADAAATAEVREIDGRRVVWFRVAGG
                     PHHGAIGLAGGATVERAVELAGQLGLPLVGELDSAGAELNEHVAALHAWGRIARALTN
                     VSGRVPMLVAVTGACVSGPALIVGLFDVVVMTEDAFAYVSGPDAASEFTGVPVDRVTL
                     GGATVHAKHSGVSTLLVEDADAARDVLAHVLSYLPDHHLADPPVAGAGDARDRWCDGA
                     ATAIPDRPTASYDVHAVIDEVCDAESFFELRPHYASNIVTGLARLDGRPIGVVANVAR
                     QRAGAIDIEASRKAARFVAMCDAFNLPIVTFVDTPGFEPGRDLEWRGMIRHGAELVHA
                     YSAATVPRLCVVMRKAFGGAYIVMDSRGIGNDLCVAWPTAEIAVMGGPAAVKILHGRR
                     LALLEGDDAVIEEQRLVGEYETAFSNPYRAAELGLVDAVIEPGETRRVLCDALEVLAT
                     KRDGQPRRRHSNTPL"
     gene            1414..2172
                     /gene="fabI"
                     /locus_tag="VFX21_16200"
     CDS             1414..2172
                     /gene="fabI"
                     /locus_tag="VFX21_16200"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX5097567.1"
                     /translation="MLLADKRVLVTGVLTDASIAFSVAKRAQEEGAEVLLTSFGRAMS
                     LTQRSARRLPTEPEIFELDVTDDAHLASLAERIGGRLDGVVHAVGFAPESCLGGGFLS
                     APWEDVAIALRVSAYSLQSLAVACRPLLGRGSSIVGLDFDNSTVAWPVYDWMGVAKAA
                     FESAARYLARDLGPDGVRVNLVAAGPLRTMAAKSIPGFEKFEEVWAERAPLGWDVKDS
                     EPVANAVVALLSDLFSATTGEAIHVDGGFHGVGA"
     gene            complement(2195..2641)
                     /locus_tag="VFX21_16205"
     CDS             complement(2195..2641)
                     /locus_tag="VFX21_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5097568.1"
                     /translation="MIRNPDHITVAVGDAPTAIEFFALLGFRKDHVAVIDGGEPAQYM
                     GMPDMKAQHITLVLEGCEPHFEIQLLAFDPTPDADPGLHPTNLRRRGYNHLALRVDDI
                     TAVTDHLTANGVKMLSDEMDYISRKLRFFEGPEGITLELVEWVTPE"
     gene            complement(2785..>3092)
                     /locus_tag="VFX21_16210"
     CDS             complement(2785..>3092)
                     /locus_tag="VFX21_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX5097569.1"
                     /translation="DAFPQLKKLKAAVVGISPDTPEKQKKFDDKYKLKFPLLADEDHK
                     VADAWGVWGEKSLYGKKFMGIVRSAFVVDERGKLAGVFYKVSPTDTVPKTEAVLRGD"
BASE COUNT          444 a         1055 c         1065 g          528 t
ORIGIN      
        1 cacaacgtca cgttgatcct tgcgcccgtg acgtgaccgc gacgccgctt cacgcgcgcg
       61 ccgatgccgc cgcgactgcc gaagtccgtg agatcgacgg tcgccgcgtt gtctggttcc
      121 gcgtggccgg cggtccgcat cacggtgcga tcggtctcgc gggaggcgcg acggtcgaac
      181 gagcggtcga gttggccggt cagctcgggc tgccgttggt cggtgagctc gattcggccg
      241 gtgcggagct caacgagcac gttgccgcgt tgcacgcgtg gggccggatc gcccgcgcgc
      301 tgaccaacgt gtcgggacgg gtcccgatgt tggtcgcggt gaccggggcg tgcgtgtcgg
      361 ggccggcgct gatcgtcggc ctcttcgacg tcgtcgtcat gaccgaggac gcattcgcgt
      421 acgtgtcggg gcccgacgcc gcgagcgagt tcacgggtgt gcccgtcgac cgagtcacgc
      481 tcggcggcgc gacggtgcac gcgaagcaca gcggcgtgtc gaccctcttg gtcgaggacg
      541 ccgacgccgc gcgcgacgtg ctcgcgcacg tcttgtcgta tctcccggat catcacctgg
      601 ccgatccgcc ggtcgccggt gccggcgatg cccgcgatcg atggtgtgac ggcgcggcga
      661 ctgcgatccc cgaccggccg accgcctcct atgacgtgca cgcggtcatc gacgaggtgt
      721 gcgacgcgga atcgttcttc gagctgcggc cgcactacgc gtcgaacatc gtcaccggtc
      781 tcgcgcgcct cgacggccgc ccaatcgggg tcgtcgccaa cgttgcccgc caacgcgcag
      841 gcgcgatcga catcgaggcg tcacgcaagg cggcacgctt cgtcgcgatg tgcgacgcgt
      901 tcaacctgcc gatcgtgacg ttcgtcgaca cacccgggtt cgagcccggc cgcgacctcg
      961 aatggcgcgg gatgattcgt cacggtgcgg aattggtgca cgcctacagc gcggcgaccg
     1021 tcccgcggct gtgcgtcgtc atgcgcaagg cgttcggcgg cgcctacatc gtcatggact
     1081 cgcgcggcat cggcaacgac ctgtgcgtcg cctggccgac cgcggagatt gcggtcatgg
     1141 gcggcccggc ggcagtgaag atcctccacg ggcgccggct cgcgttgctc gaaggcgacg
     1201 acgcagtcat cgaggaacaa cgcctcgtcg gcgagtacga gaccgcgttc tcgaacccgt
     1261 accgcgcggc cgaactgggc ctcgtcgacg ccgtcatcga gccgggcgag acgcgacgcg
     1321 tgctctgcga cgcgctcgag gtcttggcca ccaaacgcga cggtcagccc cgtcgccgcc
     1381 actccaacac accgttgtaa agcgaggcat tgcatgctct tggccgacaa acgcgtcctc
     1441 gtcaccggcg tgctcaccga tgcgtccatc gcgttctcgg tcgccaaacg cgcgcaggag
     1501 gaaggcgccg aggtgctgct gacgtcgttc gggcgggcga tgagcctgac ccaacggtcg
     1561 gcgcggcgcc tgccgaccga gccggagatc ttcgagctcg acgtcaccga cgacgcgcac
     1621 ttggcgtcgc tcgccgaacg aatcggcggc cgcctcgacg gcgtcgtgca cgccgtcggc
     1681 tttgcgcccg agtcctgctt gggcggcggg ttcctcagcg cgccgtggga agacgttgcg
     1741 atcgcgctgc gggtgtcggc gtactcgttg cagtcgctcg cggtcgcctg ccggcccctg
     1801 ctgggccgcg ggtcgtcgat cgtcggtctt gacttcgaca acagcacggt cgcgtggccc
     1861 gtgtacgact ggatgggcgt ggccaaggct gcgttcgagt cggccgcgcg ctacctcgct
     1921 cgcgatctcg ggcccgacgg cgtgcgcgtc aacctcgtcg ccgcgggtcc gttgcgcacg
     1981 atggccgcca agagcatccc cggcttcgag aagttcgaag aagtgtgggc cgaacgcgcg
     2041 ccactcggtt gggacgtcaa agactctgag ccggtcgcga acgcggtcgt ggcgctcttg
     2101 tccgacctgt tctccgcgac aaccggcgaa gccatccacg tcgacggcgg attccacggc
     2161 gtcggcgcgt aaccgcaggt cgacctcggc gccgtcattc cggcgtgacc cactccacca
     2221 gttcgagcgt gatgccctcc gggccttcga agaagcgcag cttgcggctg atgtagtcca
     2281 tctcgtcgct cagcatcttc acgccgttgg cggtcaggtg atccgtcacc gcggtgatgt
     2341 cgtcgacgcg caacgcgaga tggttgtagc cgcggcggcg caggttcgtg gggtgcagcc
     2401 ccgggtccgc gtccggtgtc ggatcgaacg cgagcaactg gatctcgaag tgcggttcgc
     2461 agccctcgag gacgagggtg atgtgctggg ccttcatgtc gggcatgccc atgtactgcg
     2521 cgggctcacc accgtcgatg acggcaacgt gatccttgcg gaagccgagc agcgcgaaga
     2581 actcgatggc ggtcggtgcg tcccccaccg cgaccgtgat gtgatcagga ttgcggatca
     2641 tgcaatagac aatgacgcgc tcacgcgttt cgtcaagatg gacgaagcgc gccggcgtcg
     2701 cccgtcgtac cgtgccacta ccgccggccc gccccggcga ggcaaggccc cggacgtgcc
     2761 ccacgctgtc cggggccgac gccgtcaatc gccgcggagg acggcctccg tcttggggac
     2821 ggtgtccgtc gggctgacct tgtagaagac gcccgcgagc ttgcccctct cgtcgacgac
     2881 gaaagcgctg cggacgatgc ccatgaactt cttgccgtag agcgacttct cgccccacac
     2941 gccccacgca tccgcgacct tgtggtcttc gtcggcgagc agcgggaact tcagcttgta
     3001 cttgtcgtcg aacttcttct gcttctcggg agtgtcgggg ctgatcccga cgaccgcggc
     3061 cttcagcttc ttcagctgcg ggaaggcgtc ac
//
LOCUS       DASUGA010000824         2883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_377278, whole genome shotgun sequence.
ACCESSION   DASUGA010000824 DASUGA010000000
VERSION     DASUGA010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377278"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFX21_16215"
     CDS             complement(<1..206)
                     /locus_tag="VFX21_16215"
                     /inference="COORDINATES: protein motif:HMM:NF018299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX5097570.1"
                     /translation="MNVYRDVSEIPDSEGRAIAIGAFDGVHLGHRAVLRLVRDLAHAR
                     GISSTVLTFDRHPAEVVRPESAPR"
     gene            complement(203..1087)
                     /gene="truB"
                     /locus_tag="VFX21_16220"
     CDS             complement(203..1087)
                     /gene="truB"
                     /locus_tag="VFX21_16220"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX5097571.1"
                     /translation="MIDGLVVVDKPAGWTSHDVVGKLRKIYGQRRVGHAGTLDPDATG
                     VLLVGLGRVTRLLRFLQEAGKSYRGCVVFGVATDTLDAAGAVLERHEMPITREQLEQA
                     SKAFVGDIEQVPPMVSALKVGGKRLHALAREGKTVDRAPRPVHIARLTVDGFEPGAYP
                     EATITVECSSGTYIRTLAADLGTALGGCAHLGGLRRLTVGSFGLDEAHTIEQIEAHPA
                     AHAITPAAAMRDLTPVTVDDELARAVGHGATFAATAIVTTDEPGPFAIVDHSGELLAV
                     YERKGKGVKPAVVLAAES"
     gene            complement(1103..2068)
                     /locus_tag="VFX21_16225"
     CDS             complement(1103..2068)
                     /locus_tag="VFX21_16225"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEX5097572.1"
                     /translation="MDEISEALEKAAGAIRNARSVALACHVNPDGDALGSTLGLMHSL
                     RAAGRPCVASFSEPFVVAPHYRELPGLDLLVPPDQFPAEPDVMVTFDCGSLSRLGDLQ
                     PAAKAASELIVLDHHVSNQCFGSINVIDPKAAASGVLVRRLISALDLPLTRDAAVCLY
                     AALVCDTGRFQYESTTPEVFDFARELSEFELPIAQLCRTLFEEHRFAYLQLLSEVLAT
                     TTLVPEKRFVWTKVTQAQLAKHAVTYEEIEGLIDVVRGTREADVACVLKEAPDQTWRV
                     SLRSLGAVDVCRIAEAQGGGGHRFAAGFTSDEPAALVVARILGEL"
     gene            complement(2071..2466)
                     /gene="rbfA"
                     /locus_tag="VFX21_16230"
     CDS             complement(2071..2466)
                     /gene="rbfA"
                     /locus_tag="VFX21_16230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX5097573.1"
                     /translation="MVDNRRQYPRTARLNESLLHVLADELERMSDPRLELVTLTGVDV
                     SRDLAHARVYYSTLALETTEHTDDLADNVAAGLAAATPHFRSAVGRQLRIRQVPALDF
                     RVDPGIVAGQRVEEILRGDRRLADDEQEL"
     gene            complement(2469..>2883)
                     /locus_tag="VFX21_16235"
     CDS             complement(2469..>2883)
                     /locus_tag="VFX21_16235"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEX5097574.1"
                     /translation="ARNSRPRPDVVSVVQQGLTEISPHRALMRAAERECDTGVVHAAA
                     VRFDLHIPQSRSLKAKRAAIRPIVDGLRHRFHVSVAEVEFLDQWQRSAIGVALVSNSA
                     PHIEEVIDEIERFVFAAADVEVLATDVTWLEPETA"
BASE COUNT          457 a          959 c         1026 g          441 t
ORIGIN      
        1 aacctgggcg ccgactcggg ccgcaccact tccgcgggat ggcggtcgaa ggtgagcacc
       61 gtcgacgaga tgccgcgcgc gtgcgcaagg tcgcgcacga gccggaggac ggcgcgatgg
      121 ccgaggtgca cgccgtcgaa tgcgccgatc gcgatcgcgc gtccttcgct gtcggggatc
      181 tcggagacgt cgcggtagac gttcatgact cggccgcgag gacgacggcg ggcttcacgc
      241 ctttcccctt gcgttcgtac acggccagga gctctcccga atggtcgacg atcgcgaacg
      301 gcccgggttc gtcggtggtc acgatcgccg ttgcggcaaa cgttgcaccg tggccgacgg
      361 cgcgcgccaa ctcgtcgtcg accgtgaccg gagtcaggtc gcgcatggcc gccgcgggcg
      421 tgatcgcgtg ggcggccggg tgggcctcga tctgctcgat cgtgtgcgcc tcgtcgagcc
      481 cgaacgagcc aaccgtgagg cgccgcagcc cgcccagatg ggcgcagccc ccgagcgccg
      541 tgccgaggtc ggcggcgagc gtgcgtatat aggtgccgga cgagcactcg accgtgatcg
      601 tcgcctcggg atacgcgccg ggctcgaaac cgtcgaccgt caggcgggcg atgtgcaccg
      661 ggcgcggcgc gcggtcgacc gtcttgcctt cgcgcgcgag cgcgtgcagt cgcttgccgc
      721 cgaccttgag cgcgctcacc atcggcggga cttgttcgat gtcgccgacg aacgccttgc
      781 tcgcctgttc gagctgttcg cgtgtgatcg gcatctcgtg tcgttcgagc accgcgccgg
      841 ctgcgtcgag cgtgtcggtc gcgacgccga acacgacgca gccgcgatag gacttccccg
      901 cttcctgcag gaaccgcaac agccgcgtga cgcggccgag gccgacgagc agcacacccg
      961 tggcgtccgg gtcgagcgtg cccgcgtgac cgacgcgccg ctgcccgtag atcttgcgca
     1021 gcttgccgac gacatcgtgc gaggtccacc ccgccggttt gtcgacgacc accaggccgt
     1081 caatcacgat ggccagtgtg tgctagagct cgccgaggat gcgagccacg accagggcgg
     1141 cgggctcgtc gctcgtgaag ccggcggcga agcggtggcc cccgccgccc tgggcctcgg
     1201 cgatgcgaca gacgtcgacc gcgccgagcg accggaggct gacccgccac gtctggtcgg
     1261 gcgcttcttt gagcacacag gcgacgtcgg cctcgcgggt gccgcgcacg acgtcgatga
     1321 ggccttcgat ctcctcgtag gtgacggcgt gcttcgccag ctgcgcttgc gtgaccttcg
     1381 tccacacgaa gcgcttctcg ggcacgagcg tcgtcgtggc caacacctcg gagagcagtt
     1441 gcaggtacgc gaaccggtgc tcctcgaaca gcgtgcggca caactgcgcg atcggcaact
     1501 cgaactccga gagctcacgc gcgaagtcga acacttccgg cgtcgtggac tcgtactgga
     1561 agcgtccggt gtcgcacacg agcgcggcgt acaggcacac cgcggcgtcg cgcgtcagcg
     1621 ggaggtcgag cgcagagatc aggcgccgta ccaacacgcc actcgcggcg gccttcgggt
     1681 cgatgacgtt gatcgatccg aagcactggt tcgagacgtg gtggtcgagg acgatcaact
     1741 cggacgccgc tttcgcggcc ggttggagat cgccgagacg agacaacgaa ccgcagtcga
     1801 aggtgaccat gacgtcgggt tccgcgggga actggtcggg cggcacgagc aagtcgagac
     1861 cgggcagctc gcggtagtgc ggcgcgacga cgaacggttc cgagaacgac gcaacacatg
     1921 gacgtccggc cgcccgtagc gagtgcatca agccgagtgt cgagccgagc gcgtcgccgt
     1981 cggggttcac gtggcaggca agggcgaccg agcgggcgtt gcggatcgcg cccgcggcct
     2041 tctccagagc ctcgctgatc tcatccatgg tcatagctct tgttcatcat cggccagccg
     2101 gcggtcgcca cgaaggatct cttctacccg ctgaccggcc acgatccccg gatccacgcg
     2161 aaagtcgagg gccgggacct gccggatccg gagctgacgg cccaccgcgg accggaagtg
     2221 gggcgtggcg gcggccaggc cggcggcgac gttgtcggcc aggtcgtcgg tgtgctcggt
     2281 cgtctcgagc gcgagggtcg agtagtagac acgggcgtgg gcgaggtcgc gggagacatc
     2341 cactcctgtc aacgtcacca actccaggcg ggggtcggac atgcgctcca attcgtcggc
     2401 gagcacgtgg aggagcgact cgttcaggcg cgcggtacgg gggtattggc ggcggttgtc
     2461 caccatcgtc aggccgtctc cggctcgagc cacgtgacgt cggtggcgag cacctcgacg
     2521 tcggccgccg cgaacacgaa gcgctcgatc tcgtcgatga cctcttcgat gtgcggggcc
     2581 gagttggaca cgagcgcgac gccgatcgcg ctgcgctgcc actgatcgag gaactcgact
     2641 tcggccaccg acacgtgaaa acgatgccgc aatccgtcga caatggggcg gatggccgcg
     2701 cgcttcgcct tcaaggagcg gctctgcggg atgtggagat cgaagcgaac ggcggccgcg
     2761 tgcacgacgc cagtgtcgca ctcgcgttcc gccgctcgca tcaatgcgcg atgcggggag
     2821 atctccgtca gtccctgctg cacgacagac acaacgtcgg gtcgcggccg tgagttcctg
     2881 gca
//
LOCUS       DASUGA010000825         3573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_794011, whole genome shotgun sequence.
ACCESSION   DASUGA010000825 DASUGA010000000
VERSION     DASUGA010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794011"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..781
                     /locus_tag="VFX21_16240"
     CDS             116..781
                     /locus_tag="VFX21_16240"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX5097575.1"
                     /translation="MKPELDIVAELSGRIHRGELGPGDRLPPERELATSLGVSRDRVR
                     DALSALERDGYVVSKRGATGGRFVTELAAPFAAWAARTAAELDDIVDFRLAIECEACR
                     FAAVRRSRRDLDAMEQAVARLDAATTPRDYRLADVAFHAAVALAAGNDRLAVAVERAR
                     GELFEPADELWKHGRNESLADHREILAAIRARAADAAAGAMAEHIERTRQELRTLVQR
                     VSG"
     gene            836..1594
                     /locus_tag="VFX21_16245"
     CDS             836..1594
                     /locus_tag="VFX21_16245"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX5097576.1"
                     /translation="MTPFALRPGLPVGDVPATPAGPMVASGKDGVSEDTLLAIATELG
                     EPVGYIQEHGGGLVQNLVPERRNEGRQSSTSSSVRLAWHTETAFHPHKPRYLLLLCLR
                     GDPEARTLLCAIDDVLPHLSDTDVAVLREPRFRTRPDESFLEPGAVGQLGEPLAVLNG
                     DEFTYDEDLMVGMDEDAHGVLARLGAAVHAHATALTLEAGDLLIVDNHRVVHGRSSFH
                     ARYDGTDRWLQRTFVVEHLPPPAERDGHIITTRF"
     gene            1696..2184
                     /locus_tag="VFX21_16250"
     CDS             1696..2184
                     /locus_tag="VFX21_16250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097577.1"
                     /translation="MPRIPVVSAAAERVREFADSLLVQGRTTVTEVVDNVRIRIEVLP
                     TMPTAMVDELRRHLNMSELATKDDVEHESRMAGRRIATLMESFVAEQRARDDALLESV
                     RNEIREELNGYSEAAKDGVGVDFGDVGALDYDDDEADELDLEELSSFDGLDDEDDETF
                     AV"
     gene            complement(2209..2284)
                     /locus_tag="VFX21_16255"
     tRNA            complement(2209..2284)
                     /locus_tag="VFX21_16255"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2249..2251),aa:Thr,seq:cgt)
     gene            complement(2312..3400)
                     /locus_tag="VFX21_16260"
     CDS             complement(2312..3400)
                     /locus_tag="VFX21_16260"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HEX5097578.1"
                     /translation="MWEDVVGQERAVATLERAAARPSHAYLLVGPRGSGVETAAREFA
                     ALMIGAGGDERALTLVRRGMHPDVVEFEPGAASYSVKDDVRARILPEAMRAPIEADRK
                     VLLILEAEKLRGNQNEAANAMLKTLEEPPPRTVIILVTSIPEDLLPTIRSRCQQIDFE
                     PTTDDVVAAALAREGVDSGRAQLAASLSGGQLARARAFAGPLASFRATFARAPARIDG
                     TGAKAIAIAEELDAAVEAAAEQVAQLHREELEAFDAEMERHGYSDRDAQRMRRRIDDR
                     HKREVRRTRIDLLLEGVTVLESVYRDALAAPAPALNADQPPLAVSPRGAAEALDACRE
                     AREAILINEKGIMRLTALVMALPAATTH"
BASE COUNT          529 a         1258 c         1269 g          517 t
ORIGIN      
        1 tctacgtctg gaacgaggcg accaccgaag tgcgctggat ggcggcctgg gacgcttccc
       61 ccgacgacgt cgaagcgttc gcgagtggcg tcgcggtcgc agtggaaacg ccggggtgaa
      121 accggagctc gacatcgtcg ccgagctgag cggccgcatc catcgcggtg agctcggtcc
      181 cggtgatcgg ctcccgcccg aacgcgagct cgcgaccagt ctcggtgtga gccgcgaccg
      241 cgtgcgcgac gcgttgagtg cactcgagcg cgacggatac gtcgtgtcca agcgcggcgc
      301 gacgggcggt cgcttcgtca ccgagctcgc ggcgccgttc gcggcgtggg cggcgcgcac
      361 ggccgcggag ctcgacgaca tcgtcgactt ccggctcgcg atcgagtgtg aggcgtgccg
      421 cttcgcggcc gtgcgccgct cgcgccgcga tctcgacgcg atggagcaag cggtcgcccg
      481 tctcgacgca gcaacgacgc cgcgcgacta ccggttggcc gacgtcgcgt tccacgctgc
      541 ggtcgcgctc gcggcgggca acgaccggct cgcggttgcc gtcgagcggg cgcgcggcga
      601 gctgttcgag ccggccgacg agctgtggaa gcacgggcgc aacgagagcc tggccgacca
      661 ccgggagatc ctcgccgcga tccgagcccg ggcggcggac gcggcggccg gggcgatggc
      721 cgagcacatc gagcgcaccc gccaagagct ccgcaccctc gtccagcggg tttcgggcta
      781 aacccgggcg aactattggt cgaatattcg accaatgctg ggtaccatcg aatccgtgac
      841 gcccttcgcg ctccggcccg gcctgccggt cggcgacgtg cccgcgacgc ccgcgggccc
      901 gatggtcgcg tccggcaagg acggcgtcag cgaggacacg ctgctggcga tcgcgaccga
      961 gctcggcgaa ccggtgggct acatccagga gcacggcggc ggcctcgtgc agaacctcgt
     1021 gcccgagcgg cgcaacgaag ggcgccagtc gtcgacgtcg tcgtcggtgc ggctcgcgtg
     1081 gcacaccgag acggcgtttc atccgcacaa gccgcgctac ctgttgctgc tgtgcctgcg
     1141 cggcgatccc gaggcccgca ccttgttgtg cgcgatcgac gacgtgctcc cgcacttgtc
     1201 cgacaccgac gtcgccgtgt tgcgcgagcc ccgcttccgc acgcggcccg acgagtcgtt
     1261 cctcgaaccg ggcgcggtcg gtcagctcgg cgaaccgctc gcggtgctca acggcgacga
     1321 gttcacctac gacgaagact tgatggtcgg gatggacgaa gacgcgcacg gcgtgctggc
     1381 gcggctcggc gccgcggtcc acgcgcacgc gacggcgctc acgctcgaag ccggcgacct
     1441 cctcatcgtc gacaaccacc gcgtcgtgca cggccgcagc tcgttccacg cgcgctacga
     1501 cggcaccgat cgctggctcc agcgcacctt cgtcgtcgag cacctgccgc cgcccgccga
     1561 gcgcgacggc cacatcatca ccacccgctt ctgagcggcg ctccggcgcg gcaagcctgc
     1621 gagtcgcggc gggtccgcgg ttcggtcgcc cggcggtcgt agcctttacg gcgagcccgg
     1681 cggggaggtt cgtcgatgcc gcggatcccg gtcgtcagcg ccgcagcaga acgtgtgcgc
     1741 gagttcgccg actcgttgct cgtgcagggg cgcacgaccg tcaccgaagt ggtcgacaac
     1801 gtgcgcatcc gtatcgaggt gctgccgacg atgcccaccg cgatggtcga cgagctccgg
     1861 agacacctca acatgtcaga gctcgcgacg aaggacgacg tcgagcacga gagccgcatg
     1921 gcgggccggc ggatcgccac gctgatggag agcttcgtcg ccgagcagcg cgcgcgtgac
     1981 gacgcgctgt tggagtcggt gcgcaacgag atccgcgagg agctgaacgg gtactcggag
     2041 gccgcgaagg acggcgtcgg cgtcgacttc ggcgacgtcg gcgcgctcga ctacgacgac
     2101 gacgaagccg acgagctcga cctcgaagag ctctctagct tcgacggtct cgacgacgag
     2161 gacgacgaga ccttcgccgt ctgagcgatc gcgttcgcac gcgcgaagtg gagccggaga
     2221 ggggattcga acccctgacc tgctcattac gagtgagctg ctctaccgac tgagctaccc
     2281 cggcgaggga gccgacagtg tacccgggtg gtcagtgggt cgtggcagcc ggcagcgcca
     2341 tgaccagcgc ggtcaggcgc atgatgccct tctcgttgat caggatcgct tcgcgcgctt
     2401 cacggcacgc gtcgagtgcc tcggctgcac cacgcggcga caccgcgagc ggaggctggt
     2461 cggcgttgag cgcaggcgcg ggcgcggcaa gtgcgtcccg gtacaccgac tccaagacgg
     2521 tgacaccttc gagcaggagg tcgatgcggg tgcggcgcac ttcgcgcttg tgccgatcgt
     2581 cgatgcgccg gcgcatgcgt tgcgcgtcgc ggtcggagta gccgtggcgc tccatctcgg
     2641 cgtcgaacgc ctccagctcc tcgcggtgga gctgcgcgac ttgctcggcg gccgcctcga
     2701 ccgcggcgtc gagctcctcg gcgatcgcga tcgccttggc gccggtgccg tcgatgcgcg
     2761 ccggggcccg tgcgaacgtc gcacggaacg acgccagcgg acctgcgaac gcgcgcgccc
     2821 gggccagctg cccacccgac agtgacgccg cgagctgcgc gcgccccgag tcgaccccct
     2881 cgcgtgcgag cgcggccgcg acgacgtcgt cggtcgtcgg ttcgaagtcg atttgttggc
     2941 agcgcgaccg gatcgtcggc agcaagtctt cgggaatcga cgtcaccagg atgatgaccg
     3001 tgcgcggcgg cggctcctcg agcgtcttca acatcgcgtt ggcggcctcg ttctgattgc
     3061 cgcgcaactt ctcggcttcg aggatcagca agaccttgcg gtcggcttcg atcggcgcgc
     3121 gcatcgcttc gggcaggatg cgggcgcgca cgtcgtcctt caccgaatac gacgccgcgc
     3181 cgggctcgaa ctccacgacg tcgggatgca tgccgcgccg gacgagcgtc agcgcgcgtt
     3241 cgtcgccgcc ggcaccgatc atcagcgccg cgaactctcg ggcagccgtc tcgacgcccg
     3301 agccgcgcgg cccgacgagc agatacgcgt gcgacggccg cgccgcggcg cgctcgagcg
     3361 tcgcaaccgc gcgctcctgc ccgacgacgt cctcccacat acggttgcga ccctactgag
     3421 tgagtgtgac ttcgggcagt acgtcccgga cgtgggccca gaccaggtcg gcgacttggt
     3481 cgatggtgcc ggacgcgtcg acaaccggcc actcgtacag cggcgccagc atccggtagc
     3541 cgtcgcgaac ggcctggtgg aactcgcgac ccg
//
LOCUS       DASUGA010000826         3470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2044760, whole genome shotgun sequence.
ACCESSION   DASUGA010000826 DASUGA010000000
VERSION     DASUGA010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2044760"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VFX21_16265"
     CDS             complement(<1..207)
                     /locus_tag="VFX21_16265"
                     /inference="COORDINATES: protein motif:HMM:NF042831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6766 family protein"
                     /protein_id="HEX5097579.1"
                     /translation="MTSLMRPSLRAKAESPVRRNALGLVCAAIFAVLVVALIFTGHQV
                     YNQDQLEHGGSTISLWSYFGQGHFW"
     gene            complement(241..600)
                     /locus_tag="VFX21_16270"
     CDS             complement(241..600)
                     /locus_tag="VFX21_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6582 domain-containing protein"
                     /protein_id="HEX5097580.1"
                     /translation="MATEKQKTAARKNIKKARAAQSARAHGKKVPRQTRGLSTAQQND
                     LPASAFAFPKERKEPLTDARHIRNAIARFDQVEGVTDAERDRAWKKIRSAAKKFDVEV
                     SESSWHDLFKGGKAKKR"
     gene            652..2004
                     /locus_tag="VFX21_16275"
     CDS             652..2004
                     /locus_tag="VFX21_16275"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX5097581.1"
                     /translation="MQRDEMISVATRYLDALVSHRGDDVPFAPSCRRTECGQNTGASG
                     DVVVTPVCDNLFVRSAQALCNELSELVGSLDPDAVPLCDVTKLWKTFDAIERYAAAGK
                     TLLARRVDDAGAWKASGYKTAAEAMAATSGTSITAAKQAIETSRQVAELPATADAMRA
                     GSLSPQKAEAIAAAASVVPERERELLAGAGATLREVRDECLKAKVVADGDAMYKRIHE
                     DRRVRDYTDAEGAWNLSARGTPDGGAEVRRTLDEIGDELFKEARRNGTREQREAYAFD
                     ALVEMARRARGEKPDDAKKPSAKYLGLIRADLEALRRGHAEGDELCEITGIGPIPVAR
                     AKELLTDAVLNLVITKGTDVLNVTNLHRKANAAQRAALLWESPQCRVEGCSRMRIEID
                     HRTGWAITQTTRLDDLDNLCDHHHDLKTTDGWALVEGTGRRAFVPPDDPRHPRNRPPP
                     "
     gene            complement(2024..2536)
                     /locus_tag="VFX21_16280"
     CDS             complement(2024..2536)
                     /locus_tag="VFX21_16280"
                     /inference="COORDINATES: protein motif:HMM:NF025447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4262 domain-containing protein"
                     /protein_id="HEX5097582.1"
                     /translation="MCKICDGYTPEEVTAMDAETIAEHGYLLQAVGDSDVDFQSWIYT
                     VGLLDLAGHPELIVAGAPVGRLAPVISSIARAVVDGEVFAVGDTVSMPIGIGRVGSVH
                     ERHYELDTFAHWHALQDYGAVKADDIEAVQIVLPEAICTARGRSWQPIFANPHALVRV
                     AAGPNRRGRH"
     gene            complement(2633..2779)
                     /locus_tag="VFX21_16285"
     CDS             complement(2633..2779)
                     /locus_tag="VFX21_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097583.1"
                     /translation="MTTRPFILELLEKDDPLRALVVDDAVFCGACHPRPNPVELPGVT
                     PPER"
     gene            complement(2865..>3470)
                     /locus_tag="VFX21_16290"
     CDS             complement(2865..>3470)
                     /locus_tag="VFX21_16290"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5097584.1"
                     /translation="LDVIEEDDDGSERIVQTVRAGESFGELGLVTAGHRSATVRARIR
                     SRLFAIGKGTFDRLLLDHLSLPEFAPTLHEVMALRAMPPFAHLSSTELLALLDHGEWH
                     NVQPHVDVVSQGEMGDAFYAVAAGQFEVLADDHRVGTIEPGGHFGEVALLVDVPRTAT
                     VRSVTPARVFRLERHGFEQLLADAFRTRFDGASHRVAQQRE"
BASE COUNT          607 a         1206 c         1137 g          520 t
ORIGIN      
        1 ccagaagtgt ccctgaccga agtacgacca cagcgagatc gtcgagccgc cgtgctcgag
       61 ctggtcctgg ttgtagacct gatggccggt gaagatcagc gcgacgacca gcaccgcgaa
      121 gatcgccgcg cacacgagac ccagcgcgtt gcgccgcacc ggcgactcgg ccttggcccg
      181 caggctcggc cgcatgagcg aagtcacgcc ggcgcggtac ccgacgccgc gatgcgcgaa
      241 tcagcgcttc ttggccttgc cgcccttgaa gaggtcgtgc cagctgcttt cggacacttc
      301 gacgtcgaac ttcttcgccg cggaccggat cttcttccac gcgcggtcgc gctcggcatc
      361 ggtgacgccc tcgacttggt cgaagcgcgc gatcgcattg cgtatgtgac gcgcgtcggt
      421 cagcggttcc ttgcgttcct tcgggaacgc gaacgcgctc gcgggcaggt cgttttgctg
      481 cgccgtcgag aggccccgag tctggcgcgg caccttcttc ccgtgagccc gcgcactttg
      541 cgcggcgcgc gccttcttga tgttcttgcg ggcggcggtc ttctgtttct cggtcgccat
      601 agccggacct cctgttcgcg ctctcgcgcg tacccgaagg cggatcacct aatgcagcgt
      661 gatgagatga tctcggtggc gacgcggtat ctggacgcgt tggtgagtca tcgcggcgac
      721 gatgtgccgt tcgcgccgag ctgccggcgg accgagtgcg gacagaacac cggcgcgtcg
      781 ggtgacgtag ttgtcacacc cgtttgcgac aatctcttcg tgcggagcgc gcaggcattg
      841 tgcaatgagt tgagtgaact cgtcggatcg ttggatcccg acgcggtgcc gctctgcgac
      901 gtgaccaagt tgtggaagac cttcgatgcg atcgaacggt acgcggcggc aggcaagacg
      961 ttgctcgcac gacgggttga cgacgcgggc gcatggaagg ccagcggcta caagaccgcc
     1021 gccgaggcga tggccgcgac gtcggggacg tcgatcaccg ccgcgaagca agcgatcgag
     1081 acctcacgac aggtcgcgga gctgccggcg accgcggacg cgatgcgtgc gggttcgctg
     1141 tcaccgcaga aagcggaggc gatcgcggcc gcggcttccg tcgtgccgga acgtgaacgg
     1201 gagttgttgg ccggcgcagg tgcgacgctg cgcgaagtgc gcgacgagtg cctgaaggcg
     1261 aaggtcgtcg ccgacggcga tgccatgtac aagcgaatcc acgaagatcg gcgcgtccgt
     1321 gactacaccg acgccgaagg cgcctggaac ctgagcgccc gcggcacacc cgacggcggc
     1381 gccgaagtcc gccgcaccct cgacgagatc ggcgacgaac tcttcaagga ggcgcgtcgc
     1441 aacgggaccc gcgaacaacg cgaggcctac gcgttcgacg ccctcgtcga gatggcccgc
     1501 cgcgcccgcg gcgaaaagcc cgacgacgcc aagaagccgt cggccaagta cctcgggctc
     1561 atccgcgccg acctcgaagc gctccgccgc gggcacgccg aaggcgacga actctgcgag
     1621 atcaccggca tcggacccat ccccgttgcc cgcgccaaag aactcctcac cgacgcggtc
     1681 ctcaacctcg tgatcaccaa gggcaccgac gtcctcaacg tcaccaacct gcaccgcaaa
     1741 gccaacgccg cccaacgcgc cgcgctgttg tgggagtcac cgcaatgccg cgtcgaaggc
     1801 tgctcgcgga tgcgcatcga gatcgaccac cgcacgggct gggccatcac ccagaccacg
     1861 cgcctcgacg acctcgacaa cctctgcgac caccaccacg acctcaagac caccgacggc
     1921 tgggcgttgg tcgaaggcac cggccgccga gccttcgtcc caccggacga cccacgacat
     1981 ccacgcaatc gaccgccgcc gtagcaaggt cagctccccc gggtcagtga cgtccacgtc
     2041 ggttcgggcc ggccgcgacg cgcacgagcg cgtgcggatt ggcgaagatc ggctgccatg
     2101 accgtccgcg cgcggtgcag atcgcctcgg gcaacacgat ctgcaccgct tcgatgtcgt
     2161 cggccttgac cgcgccgtag tcctgcaacg catgccaatg ggcgaacgtg tcgagctcgt
     2221 agtggcgctc gtgcacgctg ccgacccgac cgatcccgat cggcatcgag acggtgtcac
     2281 caacggcgaa gacctcgccg tcgacgacgg cccgtgcgat cgacgagatg accggcgcca
     2341 gacggccgac cggcgcgccc gcaacgatca gttcgggatg cccggccaag tcgagcagcc
     2401 caacggtgta gatccaggac tggaagtcga cgtcggagtc gcccacggct tggagcagat
     2461 aaccgtgttc ggcgatggtc tcggcatcca tcgcagtgac ttcttcgggg gtgtatccgt
     2521 cgcagatctt gcacatgtcg agaggatcgc gcgccggtgt gacagctccg ctcgcgtgcg
     2581 cacgacggac accggcgatc acacggcgac cacgcatgcc gcgacctcgg cgtcatcgct
     2641 cgggaggcgt gacacccggg agctcgaccg ggttcggtcg tgggtggcac gcgccgcaga
     2701 acacggcgtc gtcgaccacc agtgcgcgca gcgggtcgtc cttctcgagc aactccaaga
     2761 tgaacgggcg tgtcgtcacg tggttctcgc agaccgcgcg gccacaaaac cgacaggttg
     2821 cgacggcagt acgccggcag tgccaacagt ccatcccggc caagctattc gcgttgctgg
     2881 gcgacgcggt gcgacgcgcc gtcgaaacgt gtgcggaacg catccgcgag cagctgctcg
     2941 aagccgtgcc gctcgaggcg gaacacgcgg gcaggcgtga cactgcgcac tgtcgccgtg
     3001 cgcggcacgt ccacgagcaa cgcgacctcg ccgaaatgcc cgcccggttc gatcgtcccg
     3061 acccggtggt cgtcggcgag cacttcgaac tgtccggccg caacggcgta gaacgcgtcg
     3121 cccatctcac cctgggacac cacgtcgacg tgcggttgca cgttgtgcca ttcaccgtga
     3181 tcgagcagcg ccagcagttc cgtcgacgac aagtgcgcga acggcggcat ggcccgtaat
     3241 gccatgacct cgtgcaacgt cggcgcgaac tcgggcaacg acaggtggtc gagcaacaag
     3301 cggtcgaagg tgcccttgcc gatcgcgaac agccggctgc ggatccgcgc ccgcacggtc
     3361 gcactccggt gcccggccgt cacgagtccc agctcgccga acgactcccc cgcgcgcacg
     3421 gtctgcacga tgcgttcgga cccgtcgtcg tcctcctcga tcacatcgag
//
LOCUS       DASUGA010000827         4752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1665589, whole genome shotgun sequence.
ACCESSION   DASUGA010000827 DASUGA010000000
VERSION     DASUGA010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1665589"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..908)
                     /locus_tag="VFX21_16295"
     CDS             complement(12..908)
                     /locus_tag="VFX21_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009715778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5097585.1"
                     /translation="MGALDGKVIAVTGAGRGIGRAVAVAAAAEGAAVVVNDYGVSIDG
                     NEPTSEIAEEVVKEITDAGGRAAANAQSVTTMAGGASIVQQAVDTFGRIDGVVCVAGI
                     LRERMLFNMSEDEWDPVIETHLKGTFTVFRAAAPIFRQQKSGSFIGFTSGAFAASVAQ
                     ANYSAAKGGIVSLVRSAAAGMNKYGATANCIAPVAKSRMSGNVPFGLEMGEPEDVAPM
                     VCFLLSDKAREVTGQVYTVNGGRVAVWNQPEEVREMRKDGRWTVDELAARFDEVGQEP
                     MPILERLKAMEDAAKSGDKPNQ"
     gene            complement(951..2114)
                     /locus_tag="VFX21_16300"
     CDS             complement(951..2114)
                     /locus_tag="VFX21_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HEX5097586.1"
                     /translation="MWPDPSRIDLALDCRAVTFENPTGGRGAGGSTYGGRKGAPSRRI
                     EAGERLVLADLVGPGTVRHIWCTIPPAPPEVMRAFVLEVFYDGHTDPSISVPLLDFFG
                     CPLGRPVAHASMLAVAAEGRGFNAYFPMPFRDSMRVELLNGSARAAETYFQIDYTLDA
                     AAAADAGVLHVSFRRENPTALKRDFVIADDFRGAGRFLGCAIGVRPIDGGYWYGEGEV
                     KVYRDGDTELPTICGTGLEDYVGSAWGMGAHLGPYAAVPLDRRAPGKTAMSIPDFVTF
                     YRWHVPDPIVFAESLRVTIQQIGMTVFLAGQEEQMEQYLATNPPAGRGWQRDPRPGVI
                     GMGICERVDDYSAAAFVYCHDVQAVPRVDVPTAIADLARRDYEPLDPMEAFFG"
     gene            2226..2576
                     /locus_tag="VFX21_16305"
     CDS             2226..2576
                     /locus_tag="VFX21_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5097587.1"
                     /translation="MRPSGIHHVSICVTDAAAGLAFYRDKLGLTQLARPDLGPGHWLD
                     AGGQQVHLMESAEAPPRSNHFAIRVDDIDEAVTDLRAAGVEVHPIPHIEGAGRQAFLH
                     DPFGNFIELNQPDH"
     gene            complement(2583..3788)
                     /locus_tag="VFX21_16310"
     CDS             complement(2583..3788)
                     /locus_tag="VFX21_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5097588.1"
                     /translation="MVTTAPPYYDPYDAAIDADPHPVWKRLRDEAPLYYNEKHGFYAL
                     SRFADVLAASLDWETYSSARSTVLEMIDTTPGDVGDASFGMMIFMDPPGHDELRKLVS
                     RAFTPRRVAVLETRVKELCDEFLDPLVGGGSFDYLENFAALIPTMVIGALLGVPKEDQ
                     NQLRIWGDAMLHYDPTGLDEERNNAAVQLAAYMKAMVEDRAQTPRDDMVSDLLAAEIV
                     RDDGTTRRLTHEEVLAFFSLLELAGSETTARLLGWASVLLHRHPDQRALLVAQPEMTP
                     NAVEELLRYEAPSPIQARFVTRDAEWYGQTIPAYSKIALLSGSAGRDEREFEDPDRFD
                     VTRHFDRHVSFGYGIHYCIGANLARLEATIALQETLARFPEYGVDESKVEMAHTSTVR
                     GPIHVTMEI"
     gene            complement(3782..4024)
                     /locus_tag="VFX21_16315"
     CDS             complement(3782..4024)
                     /locus_tag="VFX21_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097589.1"
                     /translation="MSDNEEVHYAKLVDGLTTEDFDPGEDVIAVFSLFKIQDAEGDEG
                     WTVRSGGEPLSQEELLGVLTGLAASMKRDLAEEWEW"
     gene            4063..4563
                     /locus_tag="VFX21_16320"
     CDS             4063..4563
                     /locus_tag="VFX21_16320"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5097590.1"
                     /translation="MATYGGLRHKNAVVLVKEIPAGERAPWRALLELADEPEPLARYL
                     DDGILYGTTDDDGRPLAAVLVILEDNTTAELRAVAVAEHAQGRGIGTWLINAVHDALR
                     GRGITTVRVGTSSLATRELIFYQRLGFRLSHIDRDYFNAARGYPPGLSYEGIPLRDMV
                     WMDQTL"
BASE COUNT          776 a         1618 c         1558 g          800 t
ORIGIN      
        1 attaacgcga actattggtt cggcttgtcg ccgctcttcg ccgcgtcctc catcgccttg
       61 aggcgctcga ggatcggcat cggctcctgc ccgacttcgt cgaagcgtgc cgcgagctcg
      121 tcgaccgtcc accggccgtc cttgcgcatc tcgcgcacct cttcgggctg attccacacc
      181 gcgacgcgcc cgccgttcac cgtgtacacc tggccggtga cctcgcgcgc cttgtcggag
      241 agcaggaagc agaccatcgg cgcgacgtct tcgggttcgc ccatctcgag gccgaacggc
      301 acgttgcccg acatgcgtga cttcgcgacc ggggcgatgc agttcgcggt tgcgccgtac
      361 ttgttcatgc cggccgccgc gctgcgcacg agcgacacga tgccgccctt cgccgccgag
      421 tagttggcct gcgcgacgct cgccgcgaac gcgcccgacg tgaagccgat gaacgaaccg
      481 ctcttctgct gccggaagat cggcgccgcg gcccggaaca cggtgaacgt gcccttcagg
      541 tgggtctcga tgaccgggtc ccactcgtct tccgacatgt tgaacagcat gcgctcgcgc
      601 aggatgcccg cgacgcagac gacaccgtcg atgcggccga acgtgtcgac ggcctgctgc
      661 acgatcgacg cgccaccggc catcgtggtg accgactgcg cgttcgcggc ggcccgaccg
      721 ccggcgtcgg tgatctcctt cacgacctct tccgcgatct cactcgtcgg ctcgttgccg
      781 tcgatgctca cgccgtagtc gttgacgacc accgccgcgc cttcggcggc ggctgcgacc
      841 gcaaccgcgc ggccgatgcc acgaccggcg cccgtcaccg cgatcacctt gccgtcgagt
      901 gcacccacga gaacctccgt ctgcgtccga agcgagcgga agctatcgcg tcatccgaag
      961 aacgcttcca tcgggtcgag cggctcgtag tcgcgacggg caaggtcggc aatggcggtc
     1021 gggacgtcga cgcgcggcac cgcctgcacg tcgtggcagt acacgaaggc ggccgcgctg
     1081 tagtcgtcga cgcgttcgca gatgcccatg ccgatcacgc cgggacgcgg gtcgcgctgc
     1141 cagccgcgcc cggcgggcgg gttcgtcgcc aggtactgct ccatctgttc ctcttgtccg
     1201 gccaggaaca ccgtcatccc gatctgttgg atcgtgaccc gcagcgactc ggcgaacacg
     1261 atcgggtcgg gtacgtgcca gcggtagaag gtgacgaagt cggggatcga catcgccgtc
     1321 ttgccgggcg cccgccggtc gaggggcacc gccgcgtacg gaccgagatg cgcgcccatg
     1381 ccccacgcgc tcccgacgta gtcctcgagt ccggtgccgc agatcgtcgg caactcggtg
     1441 tcgccgtcgc gatacacctt cacttcacct tcgccgtacc agtagccacc gtcgatcggg
     1501 cggacaccga tcgcgcaacc gaggaagcgt cccgcgccgc ggaagtcgtc ggcgatcacg
     1561 aagtcgcgtt tcagcgcggt cgggttctcg cgccggaacg aaacgtgcaa cacgcccgcg
     1621 tccgcggccg ccgcggcgtc gagtgtgtag tcgatctgga agtacgtctc cgcggcgcgc
     1681 gccgaaccgt tcaagagctc gacgcgcatc gagtcgcgga acggcatggg gaagtacgcg
     1741 ttgaaaccac gcccttcggc cgcgaccgcc agcatcgacg cgtgcgcgac gggccggccg
     1801 agcggacatc cgaagaagtc gagcagcggc accgagatgc tcggatcggt gtgcccgtcg
     1861 tagaacacct cgagcacgaa cgcgcgcatg acttcgggtg gtgcgggcgg gatcgtgcac
     1921 cagatgtggc gcaccgtgcc ggggccaacg aggtcggcga gcacgagccg ttcgcccgcc
     1981 tcgatgcggc gggagggtgc gcccttccga ccgccgtagg tcgagccgcc cgcgccgcgc
     2041 ccgccggtcg ggttctcgaa ggtgacggcg cggcagtcga gcgcgaggtc gatacggctg
     2101 ggatcaggcc acatgcgccg caccgtacgg gaaatctgtc tgcacgaggc tcagtccgta
     2161 tcggggcctg tggcgaggga ctcaggtttg gctcccaccc gccgtttcgg cgttagcctt
     2221 cgaccgtgcg cccgagcggt atccaccacg tgtcgatctg cgtcacggac gcggcggcgg
     2281 gcctggcgtt ctaccgggac aaactgggcc tcacgcagct ggcacggccc gatctgggtc
     2341 ccggtcattg gctcgacgcc ggcggccagc aggtgcacct catggagtcg gcggaagcgc
     2401 cgccgcgctc gaaccacttc gcgatccgcg ttgacgacat cgacgaagcc gtgaccgatc
     2461 tccgcgccgc gggcgtcgag gttcatccca ttccgcacat cgaaggcgcc ggacgccaag
     2521 cgttcctgca cgaccccttc ggcaacttca tcgagctcaa ccagcccgac cactagggcc
     2581 gattagatct ccatcgtgac gtggatgggg ccgcggaccg ttgacgtgtg ggccatctcg
     2641 accttgctct cgtcgacgcc gtactcgggg aagcgcgcca gcgtctcttg cagcgcgatc
     2701 gtcgcttcga ggcgagcgag gttggcgccg atgcagtagt ggatgccgta gccgaacgag
     2761 acgtgacggt cgaagtggcg cgtgacgtcg aagcggtcgg gatcttcgaa ttcgcgttcg
     2821 tcacgaccgg cggatccgct caacaacgcg atcttcgagt acgcgggaat cgtctggccg
     2881 taccattccg cgtcgcgcgt cacgaaccgc gcctggatcg gtgacggcgc ctcgtaccgc
     2941 aagagctctt cgacggcgtt cggcgtcatc tcgggctgcg cgacgagcaa cgcgcgctga
     3001 tctgggtgcc ggtgcaacaa caccgaggcc cagccgagca agcgcgccgt cgtctcactc
     3061 cctgccaact cgagcagtga gaagaacgcg agcacttcct cgtgtgtcag tcggcgcgtg
     3121 gtgccgtcgt cacggacgat ctcggcggca agcaggtcgg agaccatgtc gtcgcgcggc
     3181 gtctgcgcgc ggtcctcgac catcgccttc atgtacgcgg cgagctgcac tgccgcgttg
     3241 ttgcgttcct cgtccaaccc ggtggggtcg tagtgcagca tcgcgtcgcc ccagatccgc
     3301 agctggttct gatcttcctt ggggacgccg aggagcgcac cgatcaccat cgtcggaatc
     3361 agggccgcga aattctcgag atagtcgaag gagccgccgc cgacgagcgg gtcgaggaac
     3421 tcgtcgcaca gttccttcac gcgcgtttcg agcactgcga cacggcgcgg cgtgaaggcg
     3481 cggctgacga gcttgcgcaa ctcgtcgtgg ccgggcggat ccatgaagat catcatcccg
     3541 aagctcgcat cgccgacgtc gccgggtgtc gtgtcgatca tctcgagcac ggtgctgcgc
     3601 gcggagctgt acgtctccca gtcgagcgac gcggcgagca cgtcggcgaa acggctcagc
     3661 gcgtagaagc cgtgcttctc gttgtaatag agcggagctt cgtcgcgtag ccgcttccac
     3721 accggatgcg ggtcggcatc gatcgccgcg tcgtacgggt cgtaatacgg aggggcagtc
     3781 gtcaccattc ccattcctcg gccaggtcgc gtttcatgga cgcagccaga ccagtcaaca
     3841 ccccgagcaa ctcttcctgc gagagcggct cgccgccgct gcgcaccgtc cagccctcgt
     3901 cgccctcggc gtcttggatc ttgaacaggc tgaagacggc gatgacgtcc tcgcccgggt
     3961 cgaagtcctc ggtcgtcaga ccgtcgacca acttcgcata gtgcacttct tcattgtcag
     4021 acatgcgcga catcatgacg caccgagctt ctcatgacgt agatggccac atatggcggt
     4081 ctacgtcata agaatgcggt cgtgctcgtg aaagagatac cggccggcga gcgagcgccg
     4141 tggcgcgcgc tgctcgaact cgcggacgaa cccgaaccgc tcgcgcgcta cctcgacgac
     4201 gggatcctgt acggcacgac ggatgacgac ggccgaccac tcgcggcggt gctcgtcatc
     4261 ctcgaggaca acacgacggc tgagctccga gcggtcgccg ttgccgagca cgcgcagggt
     4321 cgaggcatcg ggacctggtt gatcaacgcg gtgcacgatg cgctgcgcgg ccggggcatc
     4381 accaccgtgc gggtcggtac gtcgagtctc gcaacgcggg agctcatctt ctaccaacgg
     4441 ctcggctttc gcctcagcca catcgatcgc gactacttca acgcggcgcg cggctatccg
     4501 cccgggctgt cgtacgaagg gatcccgctg cgcgacatgg tctggatgga tcaaacgctg
     4561 tagcggttcg gacctagctc acaagcgcgg caaacgaccg gcgaattgct gaaccgcggg
     4621 agaaggtgtc cgttgttcgt ggtcttggtg cgcgttacac acaacgccgg gttggccgtt
     4681 gtacgtgccg tcgaccggcc tgtgtgccag gacacgtcta cgaaccacat aggggaccta
     4741 tgaagatcac ac
//
LOCUS       DASUGA010000828         3767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_654597, whole genome shotgun sequence.
ACCESSION   DASUGA010000828 DASUGA010000000
VERSION     DASUGA010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654597"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..741
                     /gene="merB"
                     /locus_tag="VFX21_16325"
     CDS             16..741
                     /gene="merB"
                     /locus_tag="VFX21_16325"
                     /EC_number="4.99.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF015216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018836 - alkylmercury lyase activity
                     [Evidence IEA];
                     GO_process: GO:0046413 - organomercury catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial lyase"
                     /protein_id="HEX5097591.1"
                     /translation="MPQNVPEFEFTDNAKKVRAFVFEFWCENGRGPTLLEVHQATELD
                     RRRIIQAYKELQLGIDVVVDEDTQNCNLLKAPPFSSFPSQVRAYIDDEFHSFAGCASE
                     AIAFSHMPPFKDKTVRIESHCACCFTPVTIVSKNFEIQSITPDTGVFLHISLSPYDWN
                     NVDMSRMCDSMNYVLDAEHAERYEKQTATRGILVNLDQAKMFVQNVANERMYNFHWKP
                     GTLNPRAVIKGFQMMGVDTSNWE"
     gene            complement(727..2019)
                     /locus_tag="VFX21_16330"
     CDS             complement(727..2019)
                     /locus_tag="VFX21_16330"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5097592.1"
                     /translation="MTISLDEALGRVASRSLSAHVARVAGGLRALGVEPGDAVAWQSP
                     NREEIDWCYRACWRLGATAVPLHHLAGDADVTRMLAATTPKVVIEDLDELPDGPAVEA
                     FWSDGDARAAVLFTSGTSGAPKGVVHTQRSLAFKALQMAEVHELGPDDCVLMPAPCAH
                     ISGLLNGITLPGVVPFRVVFMAKWDPAVALGLIASERVTYMVGPPTFFVSMMQQPSFS
                     TDNVASLRLISSGGAGVSEAFVADAQEAFGARVKRTYGSTEAPSVITEGRAIGPVELR
                     VDAHGELLIRGEEVALGYLDAEASAASFSADGWYRSGDLATIAPDGTVAITGRLKDIV
                     IRGGENISTAEVEQVLEAHPAVVHAVVLGEPDELMGERVVAYVETTGAVDLDTVRAWF
                     VEQQVAKFKTPERVVVVDRIPLLPTGKPDKEQLRSFPV"
     gene            complement(2016..3224)
                     /locus_tag="VFX21_16335"
     CDS             complement(2016..3224)
                     /locus_tag="VFX21_16335"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5097593.1"
                     /translation="MPAHALGSNPFADTEDQAALRQLARDVADRELAPMARHGDETEE
                     FPQWAWDGMKKAELFGITIGEKWGGAGLGDIEAAIVLEELARADVSAAILAQLIFNGP
                     PRAIQHLGSDEMKDRWLPLAASGDGLFCIGISETEAGSAVGHMRARLTPDGDGFRLNA
                     YKNYVTGGHKARACLVWCRFPDSAGTKGIGAVVVDLEDPGVTVAGTHVKMGLRATSEA
                     ELAFDDVRIDKEDVLLWGDPANSESFKTLISHINHERCGNAAMCIGAAQGALEYAIQY
                     MNERKIGDASLATLQGLQWKIADMSTQLEGARLLLQRAVHMAGPHGTPPALETAMAKT
                     AANLAAKFVCDEAIQLLGGYGFSREYPVERVYRDIRGLCIGAGTVEIQRNFVGGSVLR
                     GAKPASDGWR"
     gene            complement(3318..>3767)
                     /locus_tag="VFX21_16340"
     CDS             complement(3318..>3767)
                     /locus_tag="VFX21_16340"
                     /inference="COORDINATES: protein motif:HMM:NF016427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX5097594.1"
                     /translation="KAARHSLAGELGRTATDAEVAGATGISEGRIAVLSAHQSRPVSL
                     SAPVSENGGTLDDLLADETSESPFDAAAIATLPAVIDRVLDRIPARERDIISLRFGLG
                     ETEARSVAEIARIYGLSTERIRQIEARAMARLRALLGSAEHRELLCA"
BASE COUNT          603 a         1249 c         1257 g          658 t
ORIGIN      
        1 gttagtgtcg gggtcatgcc gcagaacgtt cccgaattcg agttcaccga caacgcgaag
       61 aaggtgcgcg ccttcgtctt cgagttctgg tgcgagaacg gtcgtgggcc gacgctgctg
      121 gaagtgcacc aggcgaccga gctcgaccgc cgccggatca ttcaggcgta caaggagctg
      181 caactcggga tcgacgtcgt cgtcgacgag gacacgcaga actgcaatct gctgaaggcg
      241 ccgccgtttt cgtcgtttcc gtcgcaggtg cgcgcgtaca tcgacgacga gtttcacagc
      301 ttcgccgggt gtgcgtcgga ggcgatcgcg ttttcccaca tgccgccgtt caaggacaag
      361 acggtgcgta tcgagtcgca ctgcgcgtgt tgcttcacgc cggtgacgat tgtctcgaag
      421 aacttcgaga ttcagtccat cacccccgac accggcgtct tcctgcacat ctcgctgtcg
      481 ccgtacgact ggaacaacgt cgacatgtct cgcatgtgcg attcgatgaa ttacgtgcta
      541 gatgccgagc acgccgagcg ctacgagaag caaaccgcga cgcgtgggat tctcgtgaat
      601 ctcgaccagg cgaagatgtt cgtgcagaac gtcgcgaacg agcgcatgta caacttccac
      661 tggaagcccg gcacgctgaa cccgcgggct gtcatcaagg gctttcagat gatgggcgtc
      721 gacacgtcaa actgggaatg aacgcagctg ctctttgtcg ggcttgcccg tcgggagcag
      781 tggaatcctg tcgaccacga cgacccgttc gggggtcttg aacttggcga cctgttgctc
      841 gacgaaccac gcccgcaccg tgtcgagatc gacggccccg gttgtctcca cgtacgccac
      901 gacccgttcg cccatcagct cgtcgggttc gccgaggacc accgcgtgca cgaccgcggg
      961 atgcgcttcg agtacctgct cgacttccgc ggtgctgatg ttctcgccgc cgcgaatcac
     1021 gatgtccttc aatcggccgg tgatggcgac ggtgccgtcg ggtgcaatcg tggcgaggtc
     1081 gccggatcgg taccagccgt cggcgctgaa ggatgcggct gaagcttctg cgtcgagata
     1141 gcccagtgcg acctcttcgc cgcggatcaa cagctcgccg tgtgcgtcga cgcggagctc
     1201 gaccggcccg atggcgcggc cctcggtgat gacgctgggt gcctcggtcg aaccgtacgt
     1261 gcgcttgacg cgtgcgccga acgcctcctg tgcgtcggcc acgaacgctt cgctcacgcc
     1321 cgcgccgccg ctcgagatca accgcagcga cgcgacgttg tcggtgctga agctcggctg
     1381 ctgcatcatc gacacgaaga aggtcggcgg cccgaccatg tacgtgacgc gttccgacgc
     1441 aatcagcccg agtgcgaccg cgggatccca cttggccatg aacaccaccc ggaacggcac
     1501 gacgccgggc aacgtgatgc cgttcagcag ccccgagatg tgcgcgcacg gcgccggcat
     1561 cagcacgcag tcgtcgggac cgagttcgtg cacttcggcc atctgcagcg ctttgaaagc
     1621 cagcgaacgc tgagtgtgga cgacgccctt cggtgcgccg ctcgttcccg acgtgaacag
     1681 cacggccgcg cgcgcgtcgc cgtcggacca gaacgcttcc actgcaggtc cgtcgggcag
     1741 ttcgtcgagg tcttcgatga cgaccttcgg tgttgtggcc gcgagcatgc gcgtcacgtc
     1801 ggcgtcgccg gcgaggtggt gcagcggcac cgcggtcgcg ccgaggcgcc agcaggcccg
     1861 atagcaccag tcgatctcct cgcggttcgg cgactgccac gcgaccgcgt cgccgggttc
     1921 gacgcccaga gcacgcagac cgccggcgac gcgcgcgaca tgcgcgctca acgaacgcga
     1981 cgcgacgcgc ccgagggctt cgtcgagcga gatcgtcaac gccagccgtc gctcgcaggc
     2041 tttgcgccgc gaagcacgct gccgccaacg aagttgcgtt gaatctcgac ggtgccggca
     2101 ccgatgcaca gcccgcgaat gtcgcggtac acgcgctcga cggggtactc gcgtgagaag
     2161 ccgtagccgc cgagcagctg gatcgcttcg tcgcacacga atttcgccgc aaggttcgcg
     2221 gcggttttcg ccatcgccgt ttccaaggcg ggcggcgtgc cgtgcgggcc ggccatgtgt
     2281 acggcgcgct gcaggagcag gcgcgcgcct tcgagctgcg tgctcatgtc ggcgatcttc
     2341 cactggaggc cttgcagcgt tgccagggac gcgtcaccga tcttgcgctc gttcatgtat
     2401 tggatcgcat attcgagcgc accctgggcg gcgccgatgc acatcgccgc gttgccgcag
     2461 cgttcgtggt tgatgtgcga aatcagggtc ttgaacgact cgctgttggc cgggtcgccc
     2521 cagagcaaca cgtcttcctt gtcgatgcga acgtcgtcga aggcgagctc tgcttcgctc
     2581 gtcgcccgca atcccatctt cacgtgggtg ccggcgaccg tgaccccggg gtcctcgagg
     2641 tcgacgacga ccgcgccgat gcccttggtt cccgcgctgt cggggaaccg gcaccacacg
     2701 aggcacgctc gcgccttgtg cccgccggtc acgtagttct tgtacgcgtt cagccggaag
     2761 ccgtcgccgt cgggagtgag gcgggcgcgc atgtggccga ccgcggatcc ggcttcggtc
     2821 tcgctgatgc cgatgcagaa caagccgtcg cccgacgcgg cgagcggcag ccaccggtcc
     2881 ttcatctcgt cggagccgag gtgctggatc gcgcgcgggg gaccgttgaa gatcagttgg
     2941 gcgaggatcg ccgccgagac gtcggcgcgc gcgagttctt cgagcacgat tgcggcctcg
     3001 atgtcaccca ggcccgcgcc gccccatttc tcgccgatgg tgatgccgaa cagctcggcc
     3061 ttcttcatcc cgtcccacgc ccattgcggg aactcttcgg tttcgtcgcc gtgacgcgcc
     3121 atcggcgcga gttcgcggtc ggcgacgtcg cgcgcgagct ggcgcagcgc ggcctgatct
     3181 tcggtgtcgg cgaacgggtt gctgccgagt gcgtgagcgg gcatgggagg aatctacagg
     3241 gctgtttcgt tacggacccg gggtacgcat gtggtccgcg tggtcagcgc aagcgcgagc
     3301 cacgcagcgg agcgggccta cgcacagagc agctcgcggt gttcagcgct gccgagcagg
     3361 gcgcgcaggc gcgccatcgc acgggcttcg atctgacgga tccgctcggt cgagagcccg
     3421 tagatgcgtg caatctccgc caccgaacga gcttctgtct cgccgagccc gaagcgcaag
     3481 ctgatgatgt cgcgctcacg cgccggaatc cgatcgagga cacggtcgat gacggccggc
     3541 agcgtcgcga tggcggcggc atcgaacggc gactcgctcg tttcgtcggc gaggagatcg
     3601 tcgagggtgc cgccgttctc ggacaccggc gcggacagcg acaccggccg actctggtgt
     3661 gccgacagaa cggcgatccg cccttcggag atccccgtcg ctccggccac ctcggcgtcg
     3721 gttgcggtgc gcccaagctc tccggcaagg gagtgccgcg ccgcctt
//
LOCUS       DASUGA010000829         5769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1488311, whole genome shotgun sequence.
ACCESSION   DASUGA010000829 DASUGA010000000
VERSION     DASUGA010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488311"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VFX21_16345"
     CDS             <1..648
                     /locus_tag="VFX21_16345"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX5097595.1"
                     /translation="VHGAMAVTDFVYQGPKRGADRAVVLAHGAGADMNAAALTTVADA
                     LADVKIPSLRFNFPYMAAGRRSPDRPPVLEGAVRDAAALLAKQSKVAPERLVLGGRSM
                     GGRVCSMVVADGLPSAGLVLLGYPLHPPGNAENLRIEHLPRITVPCLFVSGTRDSFGT
                     PDELKRHAKKIKGKVTWHWIDTGDHSFRPLKASGLTPASALEGAAGAVVSFVTAL"
     gene            840..1358
                     /locus_tag="VFX21_16350"
     CDS             840..1358
                     /locus_tag="VFX21_16350"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5097596.1"
                     /translation="MRVDRWFAFVDLSGFTSFSDEHGDEESVRVLTSFRQSVRTVATD
                     FGVRIAKWLGDGCMLVSVDPANVVAAVCRLEALVDDAELPLQIHAGMAGGAVILLEGD
                     DYTGGAVNLASRLADLAGPHQIFTTPYLATFAPEGTRCEAAGFRSVSGMHDPVEVVCL
                     GCTRMRSAEART"
     gene            1376..2203
                     /locus_tag="VFX21_16355"
     CDS             1376..2203
                     /locus_tag="VFX21_16355"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5097597.1"
                     /translation="MTAYETLELQRDGDIAWLRLNRPDKLNSFTVKMWREMRALGNEM
                     HDDPSIRCMVVIGNGRAFSSGIDTSVFTGAGADDAIQGGDDAGTRHVDPTVDGILRTQ
                     EAYSWLSDARYPTIAAVRGYAFGAGLQLAIACDIRVFARGAQVGLLEHKYGILPDLGG
                     TQRLPRIVGAGKAKELIWTTARIDADEAFRIGLCERLVDEGDLEKEVEVLASTIASAP
                     PLAVQGAKRAIEAAGRLPVAEGLVVEAEEQARCLHSNDMREAITAFVEQRAPEYNAQ"
     gene            2200..3456
                     /gene="murA"
                     /locus_tag="VFX21_16360"
     CDS             2200..3456
                     /gene="murA"
                     /locus_tag="VFX21_16360"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX5097598.1"
                     /translation="MSRYRVRPSGPLRGRVEVSGATKNAGTKQMAAALLAPGVTTLRN
                     MPVVADLDVMVDLLRSIGASVEVLPGDEVRIDAAGELRAEAPYELVKKMRASFNVLGP
                     LLARCGHARVALPGGDNIGSRKVDMHLRGLEAMGASVDVEHGFVVAQANRLAGARVVL
                     EFPSVGATENLMCAAVLAKGSTVIENAAREPEVTDLAAFLCRMGAQVIGAGTSTIEIE
                     GVDELAPVDSEIMGDRVEAGTLLMACGITGGELELVGTRQEHLEIVVMKLCEMGMRIS
                     PTPDGLWAHAPNRLQAVDIATLPHPGFATDFMPLAVALMTVAVGSAIVTENIYDGRFQ
                     FVDELARMGADVRTEGRHAVVRGVDRLSGAPVAASDVRAGAALVLAGLVADGETIVYE
                     CHHVDRGYVDLPETLRELGADVERVV"
     gene            3861..4085
                     /locus_tag="VFX21_16365"
     CDS             3861..4085
                     /locus_tag="VFX21_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX5097599.1"
                     /translation="MEEGVLEAIDAIRPALQSDGGDIVFNFIDEDYVVHVSLVGACGS
                     CPVSMMTLKAGVERIIMDRVPGITEVVADE"
     gene            complement(4131..4736)
                     /locus_tag="VFX21_16370"
     CDS             complement(4131..4736)
                     /locus_tag="VFX21_16370"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEX5097600.1"
                     /translation="MRAVRRSLLILVVLFCPLGACSHDDKSGSEPPATTARSTTSTAA
                     VTTTTAAIVTYQVQRGDTLAAIAQRFRVSVSKLVELNQLANPDLLTENQTLRIPPPPP
                     LALVVTPASAEQGRDFEFTLTGAVPLEEITFEVDSPEGKYTGPAHNAAEDGTVTAIYR
                     TSLSDEAGDYVVIARGNQGTTAQSGFVVEAIRRDTTSTTPE"
     gene            4843..>5769
                     /gene="meaB"
                     /locus_tag="VFX21_16375"
     CDS             4843..>5769
                     /gene="meaB"
                     /locus_tag="VFX21_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019354651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX5097601.1"
                     /translation="MRAADPKALVERALDGDRGALAKLLSVVEAGGDNARVAASLLHP
                     HTGRAWSIGITGAPGAGKSTLTDRLVSRIRADGPEVGVLAVDPSSPFSGGAILGDRVR
                     MQDHATDAGVFIRSMASRGHLGGLAVATPQSLRVLDAVGKPWIVVETVGVGQVEVEIA
                     GHADTTVVVVNPGWGDAVQAAKAGLLEIADVFVVNKADRPGASETVRDLEGMLELAGT
                     IPWRPPIVRTVATTGEGIDEFWAAIRQHRAHMEEGDRLARRRHERIAAEVRAIVAEQL
                     LVRAGEQCRGPAFDALVDRVAARTLDPYQAADE"
BASE COUNT          883 a         1944 c         2044 g          898 t
ORIGIN      
        1 gttcatggcg cgatggctgt aaccgacttc gtctaccaag gtcccaagcg gggcgccgat
       61 cgcgcggtgg tgcttgcgca cggtgccggc gccgacatga acgcagctgc actgacgacc
      121 gtcgccgatg cgctcgccga cgtgaagata ccgtcgctgc ggttcaactt cccgtacatg
      181 gccgcagggc ggcgcagtcc cgatcgtcca ccggtgctcg aaggcgcggt gcgcgacgca
      241 gccgcgctgc tcgcgaagca gtcgaaggtc gcgcccgaac gactcgtgct cggcggccgt
      301 tcgatgggcg gtcgcgtctg ttcgatggtc gtcgcggacg ggttgccgag cgcgggcctc
      361 gtgttgctcg gctatccgct gcacccgccc ggcaacgccg agaacttgcg catcgaacac
      421 ctgccgagga tcacggtgcc gtgcctcttc gtgagcggca cccgcgactc gttcggcaca
      481 cccgacgagc tgaagcgcca cgccaagaag atcaagggca aggtcacgtg gcactggatc
      541 gacacgggtg atcacagttt ccggccgttg aaggcgagcg gcctcacgcc cgcgagcgcg
      601 ctcgagggcg cggccggcgc cgtcgtcagc ttcgtcaccg ccctgtgaaa tgacgcggcg
      661 aattcaatga ggcggtggcc gcggtgtcct tgcgatgcgc gaagcgccgc gtcaggacgt
      721 tctcggccac tcgcggagtc ctgaggccgg ccgacaatcc gagctgtctc acgcgagcag
      781 gtcgaaggcg acaaagctgg atacgaggtc gccgggcggg ccgtgcgaga cttcgggcaa
      841 tgcgggtcga ccgctggttc gccttcgtgg acctctccgg cttcacgtcc ttcagcgacg
      901 agcacggcga cgaagagtcc gtgcgcgtgc tcacttcgtt ccgtcaatcc gtgcgcactg
      961 tcgcaaccga tttcggtgtg cgcatcgcga aatggttggg cgacggttgc atgctcgtgt
     1021 ccgtcgatcc ggcgaacgtc gtcgccgcgg tctgccgtct cgaagcgttg gtcgacgacg
     1081 cggagttgcc gttgcagatt cacgcgggca tggccggcgg cgcggtcatc ctcctggaag
     1141 gtgacgacta caccggcggc gcggtgaacc tcgcgtcgcg cctcgcggat cttgccggtc
     1201 cgcaccagat cttcacgacg ccgtacctgg caacgttcgc gcccgagggc acacgctgcg
     1261 aagcggccgg gttccgctcg gtgtcgggga tgcacgaccc ggtggaagtc gtatgcctgg
     1321 gctgcacgcg catgaggagc gccgaggctc gtacgtagca gtaccgttgc tgctcatgac
     1381 ggcctacgag acgctcgagc tccaacgcga cggcgacatc gcctggttgc gcctgaaccg
     1441 gcccgacaag ctcaacagct tcaccgtgaa gatgtggcgc gagatgcgcg cgctcggcaa
     1501 cgagatgcac gacgacccct cgatccgctg catggtcgtc atcggcaacg gacgtgcgtt
     1561 ctcgtccggt atcgacacgt cggtgttcac gggtgccggc gcggacgacg cgatccaggg
     1621 cggtgacgac gccggcactc gccacgtcga tccgacggtc gacggcatcc tgcgcacgca
     1681 ggaggcgtac agctggctct cggacgcgcg gtacccgacg attgccgcgg tgcgcggcta
     1741 cgcgttcggc gccggcctcc agctcgcgat cgcgtgcgac attcgcgtgt tcgcgcgcgg
     1801 cgcgcaggtc ggcctgctcg agcacaagta cggcatcctc cccgacctcg gcggtacgca
     1861 acggttgccg cgcatcgtcg gcgcgggcaa ggccaaggaa ctgatctgga cgacggcgcg
     1921 catcgacgcc gacgaagcgt tccgcatcgg cctgtgcgaa cggctcgtcg acgaaggcga
     1981 tctcgaaaag gaagtcgaag tgctcgcgtc gacgatcgcg agcgcgccgc cgctcgcggt
     2041 gcaaggcgcg aagcgcgcga tcgaagccgc cggtcgtctc ccggtcgcgg aagggctggt
     2101 cgtcgaagcc gaggagcagg cgcggtgcct gcactcgaac gacatgcgcg aagcgattac
     2161 cgcgttcgtc gagcagcgcg cgccggagta caacgcccag tgagccgcta ccgagtccgc
     2221 ccgagcgggc ctcttcgcgg tcgggtcgag gtgtccggcg cgacgaagaa cgccggcacc
     2281 aagcagatgg cggcggcctt gctcgcgccg ggcgtcacga cgctgcgcaa catgccggtc
     2341 gtggccgacc tcgacgtcat ggtcgacctg ttgcggtcca tcggtgcgtc ggtagaggtg
     2401 ctcccgggtg acgaggtgcg gatcgacgcg gcgggggagc tgcgggccga ggccccgtac
     2461 gagctggtca agaagatgcg ggcgtcgttc aacgtgctcg gcccgctcct cgcccgctgc
     2521 ggtcacgcgc gagtggcgct gcccggcggc gacaacatcg gcagccgcaa ggtcgacatg
     2581 cacctgcgcg gcctggaagc gatgggtgcg tccgtcgacg tcgagcacgg cttcgtcgtc
     2641 gcgcaggcga accggctcgc gggcgcgcgc gtcgtgctcg agttccccag cgtcggcgcg
     2701 accgagaacc tcatgtgcgc ggccgtgctc gcgaagggct cgacggtcat cgagaacgcg
     2761 gcccgcgagc ccgaggtcac cgacctcgcc gcgttcttgt gccgcatggg cgcgcaggtg
     2821 atcggcgccg ggacgtcgac gatcgagatc gagggcgtcg acgagctcgc gccggtcgac
     2881 agcgagatca tgggtgaccg cgtcgaggcg ggcacgctgt tgatggcgtg cggcatcacc
     2941 ggcggtgagc tcgagctggt cggcacccgc caggagcacc tcgagatcgt cgtcatgaag
     3001 ctgtgcgaga tgggcatgcg catctcgccg acccccgatg gactgtgggc gcacgccccg
     3061 aaccggctgc aagccgtcga catcgcgacg cttccgcacc cggggttcgc gaccgacttc
     3121 atgccgctcg cggtcgcgct catgacggtc gccgtcggca gcgcgatcgt caccgagaac
     3181 atctacgacg gtcgcttcca gttcgtcgac gagctcgccc gcatgggtgc cgacgtgcgc
     3241 accgagggtc gccacgccgt cgtccgcggg gtcgaccgcc tgtccggcgc gccggtggcc
     3301 gcctccgacg tccgggccgg cgccgcgctc gtgctcgcgg gcctcgtcgc cgacggcgag
     3361 acgatcgtct acgagtgcca ccacgtcgac cgcggctacg tcgacctccc cgagaccctc
     3421 cgcgagctcg gcgccgacgt cgaacgcgtc gtctgaccag gcgggcgcga gaaatccaaa
     3481 ttcccgcgca acttttgagc accgggtgac gtctgtacgt gtgaaagggc gccacgggaa
     3541 accgaggcag gcaagccagc aagccggcga ggtcacgacg ggcaacgggt ggcgacgagc
     3601 tcctcgggaa ccgagccgga gcacgagata cgggcccccg gtggttgtgt ccccgcgccc
     3661 ccatccgggg tcccgtaagt tctcggagag accagcgagc cccgcatacc cctgcggaac
     3721 ttctccggcg gcgtccagga cgggcgccgc cggttcgcgt ccgggaagca gccggtagcc
     3781 tgcggtgttg gaaacactgt ccgtgccccc cgcacccgga gccgtcgatg tcgaccgaac
     3841 tcgccgaaga aatggccgcg atggaagaag gcgttctcga ggcgatcgac gccatccgcc
     3901 ccgccctcca gagcgacggc ggcgacatcg tgttcaactt catcgacgag gactacgtcg
     3961 tccacgtttc cctggtcggc gcgtgcggct cgtgccccgt ctcgatgatg acgctgaagg
     4021 ccggtgtgga gcggatcatc atggaccgcg tccccggcat caccgaagtc gtcgccgacg
     4081 agtagtccgg ggcctcgggc accgagatcc gaatctcgat cggaacgccg ttactcggga
     4141 gtcgtcgacg tcgtgtcgcg acgtatcgct tcgacgacga agccgctttg cgcggtggtg
     4201 ccctggttcc cgcgcgcaat gacgacatag tcgccggctt cgtcgctgag ggacgtgcgg
     4261 tagatcgcgg tcaccgttcc gtcttcggct gcgttgtgcg cgggcccggt gtacttgccc
     4321 tcgggtgagt cgacttcgaa ggtgatctct tccaacggca cggcaccggt gagcgtgaac
     4381 tcgaagtcgc gtccttgctc cgcactcgcc ggtgtgacga ccagcgccaa cggtggaggg
     4441 ggcgggatcc ggagcgtctg gttctccgtg agcagatctg gattcgcgag ctgattgagc
     4501 tcgacgagct tcgagaccga gacgcggaat cgctgcgcga tggctgccag cgtgtcgccg
     4561 cgctgcacct gatacgtgac gatcgcggca gtcgtcgtcg tcacggccgc cgtgctcgtc
     4621 gtcgaacgtg ccgtcgtcgc cggcggttcc gacccggact tgtcgtcgtg gctgcacgcg
     4681 ccgaggggac agaagagcac gacgagaatg agcagtgacc gccgcaccgc gcgcatcggt
     4741 actcaccttc gggccgccgt cgacggtcgt ctctgacgtt cccgagtcag gcgggcccga
     4801 accatgcgag cggcgctcgg atccgcacta tcttccaggc gcatgcgcgc cgccgatccg
     4861 aaggctctgg tcgagcgggc gctcgacggc gatcggggcg cgctggccaa gctgctctcg
     4921 gtcgtcgaag cgggcggcga caacgctcgc gtcgcggcct cgctcctgca tccgcacacc
     4981 ggccgcgcgt ggtcgatcgg catcaccggc gcgcccggcg cggggaagtc gacgctcacc
     5041 gaccggttgg tgagtcgcat ccgggccgat ggtcccgagg tcggagtgct cgcggtcgac
     5101 ccgagcagcc cgttcagcgg tggcgcgatc ctcggtgatc gggtgcgcat gcaagaccac
     5161 gccaccgacg ccggtgtgtt catccgttcg atggcgtcac gtggtcacct cggcggactc
     5221 gcggtcgcga cgccgcagtc gttgcgcgtg ctcgacgcgg tcggcaagcc gtggatcgtc
     5281 gtggagacgg ttggcgtcgg tcaggtcgaa gtcgagatcg cgggccacgc ggacacgacc
     5341 gtcgtcgtgg tgaaccccgg atggggtgac gcggtacagg ccgcgaaggc cggtctgctc
     5401 gagatcgccg acgtgttcgt cgtgaacaag gccgaccggc cgggtgcgtc cgagacggtg
     5461 cgcgacctcg aaggcatgtt ggagctcgcg ggcacgatcc cctggcgccc accgatcgtg
     5521 cgcacggtcg cgacgaccgg cgaaggcatc gacgagttct gggccgcgat ccgacaacac
     5581 cgcgcgcaca tggaagaggg tgaccgattg gcccgccgcc gccatgaacg catcgccgcg
     5641 gaggtgcggg cgatcgtcgc cgagcaactg ctcgtgcgcg cgggcgagca gtgccgaggc
     5701 cctgcgttcg acgcgttggt cgaccgggtc gcggcccgca ccctcgatcc gtaccaagcc
     5761 gccgacgag
//
LOCUS       DASUGA010000830         2979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_934626, whole genome shotgun sequence.
ACCESSION   DASUGA010000830 DASUGA010000000
VERSION     DASUGA010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934626"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VFX21_16380"
     CDS             <1..338
                     /locus_tag="VFX21_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003996399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5097602.1"
                     /translation="QLDEISRDELAARLDRGEVIVVDVRPSAEYEAGHIAGARSIPAK
                     RLAREVATLPADVEVVAYCRGPYCVFADDAVRLLKRRGLKARRLEDGYPEWRDAQLPV
                     ETAVNEATA"
     gene            364..969
                     /locus_tag="VFX21_16385"
     CDS             364..969
                     /locus_tag="VFX21_16385"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5097603.1"
                     /translation="MDVEELVDTDQLRAQVRQKYREVATDPHRTYHFHTGRPLAARLG
                     YDTVAVDALPDRAVESFAGVGNPFSLRHLEPGERVVDVGSGAGFDSFIAAGQVGPSGV
                     VVGVDMTTEMLEKSTVTTRELGLGHVEFRAGYAEQLPVEDAWADVVVSNGVINLCADK
                     RAVFAEIHRVLRPGGWLQFADIANGRPVPPEALRDIDLWTG"
     gene            1006..1140
                     /locus_tag="VFX21_16390"
     CDS             1006..1140
                     /locus_tag="VFX21_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097604.1"
                     /translation="MLEASGFEHVLIGDPVDTFGGAKGEASARTFEVYGYAFLARKGL
                     "
     gene            1165..1977
                     /locus_tag="VFX21_16395"
     CDS             1165..1977
                     /locus_tag="VFX21_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5097605.1"
                     /translation="MNPNQALWEKGDFTRIAESMRASGDELVERIGVTEGLDVLDLGC
                     GDGTTALPEARRGANVLGVDIARNLVEAGNKRAADEGLTNLRFQHGDASALTDLADDQ
                     FDLVVSIFGAMFAPNPFDVAKEMVRVTRPGGRIVMGNWIPGDPTLVAQILRISSAYSP
                     PPPEGFVSPMTWGVVDDVVARFTAAGVPESNIRCEPDTFVFEAPVSPTEYLTWFRDYY
                     GPTMNAFEAAMTDGRADELFKELDELFNEKNASGDPNTTSIPATFLCIEVNV"
     gene            2259..2501
                     /locus_tag="VFX21_16400"
     CDS             2259..2501
                     /locus_tag="VFX21_16400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX5097606.1"
                     /translation="MPDVSATDAARNFADLLDAVEHRGEHFTIVRRGRAVASLEPTSQ
                     GRGSDVKAVLARHRPDAGWMRDLDAVRTLVELDERA"
     gene            2498..2890
                     /locus_tag="VFX21_16405"
     CDS             2498..2890
                     /locus_tag="VFX21_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020136334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX5097607.1"
                     /translation="MTQLLLDTTFLVDVERGADLARVIADDDDVAIAAITIAELRVGV
                     LLADGRRRRARSSYVEELIDAIPVIPYDLRVAEAHAQLLIAVRQQGRPRGAHDLIVAA
                     TARSTGRVVLTADASAFADLPDVEIRPH"
BASE COUNT          533 a         1015 c          952 g          479 t
ORIGIN      
        1 agcagctgga tgagatcagt cgtgacgagc tggccgctcg tttggatcgc ggcgaagtga
       61 tcgtcgtcga tgtccggccg agtgccgagt acgaggcagg acacatcgcc ggtgcgcgtt
      121 cgatccccgc gaagcgcctc gcgcgagaag tcgcgacgct tcccgccgac gtcgaggtcg
      181 tcgcctactg ccgaggtccg tactgcgtct tcgctgacga cgcggtgcga ctgctcaagc
      241 gccgcgggct caaggcgcga cgtctcgagg acggctaccc cgaatggcgc gacgcccagc
      301 taccggtcga aaccgcagtg aacgaagcaa cggcgtaaag cgctgttcgc aaaggagcgt
      361 gcgatggacg ttgaagagct cgtcgacacc gaccagctac gagcgcaagt acgccagaag
      421 taccgcgaag tcgccaccga tccacaccgc acgtaccact tccacacggg acgaccgctc
      481 gcagctcgcc tcggctacga cactgtcgcc gtcgacgcgc tgccggaccg tgcggtggaa
      541 tcgttcgccg gcgtcggcaa tcccttctca cttcgacacc tcgagccggg tgaacgcgtc
      601 gtcgacgtcg ggtcgggcgc agggttcgat tccttcatcg ccgccggcca ggtcggccct
      661 tcgggagtcg tcgttggcgt cgacatgacc acagaaatgc tcgagaagtc gaccgtcacc
      721 acgcgcgagc tcgggctcgg gcatgtcgag ttccgcgccg gctacgcgga acagcttccg
      781 gtcgaggacg catgggccga cgtcgtcgtc tcgaacggcg tcatcaactt gtgcgccgac
      841 aagcgcgccg tcttcgcgga gatccatcgc gtgctgcgtc ccggcggatg gctgcagttc
      901 gccgacatcg cgaacgggcg gcccgttcca cccgaagcgc tccgtgacat cgatctctgg
      961 accggctgaa tcgccggcgg gctgccccgt gagggctggc gcaccatgct cgaagcaagc
     1021 ggattcgaac acgtgctgat cggcgacccc gtcgacacct tcggcggcgc aaaaggcgaa
     1081 gcgagcgcgc gcaccttcga ggtctacggc tacgcgttcc tcgcacgaaa aggtctctga
     1141 acaaacacaa aggaggggat ggacatgaat cccaaccaag cactgtggga gaagggtgac
     1201 ttcacgcgca tcgccgagag catgcgcgcg agcggcgatg agctcgtcga gcgaatcggc
     1261 gtgaccgaag gactcgacgt gctcgacctc ggctgcggag acggaaccac cgcgttgccc
     1321 gaggctcgac ggggcgccaa cgtcctcggc gtcgacatcg cccggaacct cgtcgaggcc
     1381 ggcaacaagc gcgccgcgga cgaaggcctc acgaacctcc gattccagca cggcgacgct
     1441 tccgcgctga ccgacctcgc cgacgatcag ttcgacctcg tcgtgtcgat cttcggagcg
     1501 atgttcgcgc ccaacccctt cgacgttgcc aaggagatgg tgcgagtgac gcgacccggc
     1561 ggccgcatcg tcatggggaa ctggatcccc ggcgatccga cgctcgtcgc acagatcctg
     1621 aggatcagct ccgcctactc accgcctccg cccgaaggtt tcgtcagccc gatgacttgg
     1681 ggcgtggtgg acgacgtcgt cgcgcgcttc accgccgccg gtgttcccga gagcaacatc
     1741 aggtgcgagc ccgacacctt tgtgttcgag gcacccgtgt cgccgaccga gtacctgacc
     1801 tggttccgtg actactacgg cccgaccatg aacgcgttcg aagcggcaat gaccgacggc
     1861 cgcgctgacg aactcttcaa ggagctcgac gagctcttca acgagaagaa cgcgagcggc
     1921 gacccgaaca ccacctcgat ccccgcaacg ttcctctgca tcgaggtgaa cgtctaggac
     1981 tgcgcgtcgt gcgacgatgg cggtagagcc gtgccggcca gcctggcgac tctcggcccc
     2041 aaccacaatg gcacgtgctc gagccgcgtc ttgcccatca atttgcccat caactcgacg
     2101 tcaaagtgag acacaccgcg acgcaccgag tcctagcgga gatcaccgga tcgtcgcgtc
     2161 aggtgcgtga gaggtgtgcg gctgccttct ccgtccgtcc ctgacgccga gtcgaagtaa
     2221 gtgatggcgg gcagcccgga atcggataat ctgatttcat gcccgatgtc agcgccaccg
     2281 acgcggcccg caacttcgcg gatctgctcg acgcggtcga acatcgcggg gagcacttca
     2341 cgatcgttcg gcgcggcaga gcagtcgcgt cgctcgagcc gacgtcccag gggcggggat
     2401 ccgacgtcaa ggccgtgctc gcgcgtcacc ggcccgacgc cgggtggatg cgtgacctcg
     2461 acgcggtccg aaccctggtc gaactcgacg agcgtgcgtg acccaactcc tgctcgacac
     2521 gacctttctc gtcgacgtcg agcgaggcgc tgatctcgca cgtgtgatcg cggatgacga
     2581 cgacgtggcg atcgctgcga tcaccattgc ggagctccgc gttggagtgc tcctcgcgga
     2641 cggtcgccgg cgacgtgctc gatcgtcata cgtcgaagag ctcatcgacg cgattccggt
     2701 cattccctac gaccttcgag tcgcggaagc tcatgcacaa cttctgatcg cggtgcgcca
     2761 gcaaggtcga cctcgcggtg cccacgacct gatcgtcgcc gccacggcgc gatccacggg
     2821 tcgcgtcgtt ctcaccgccg acgcgagcgc gttcgccgac cttccggacg tcgagatccg
     2881 gccgcattga tcggcggcac ttgccgcaag gtcgccggac gcgccccccg cgagtcgtcg
     2941 acgcggtcga gacgcgcgtg ctccaggtgc gcgtcacag
//
LOCUS       DASUGA010000831         6160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_378797, whole genome shotgun sequence.
ACCESSION   DASUGA010000831 DASUGA010000000
VERSION     DASUGA010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6160
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378797"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VFX21_16410"
     CDS             complement(<1..472)
                     /locus_tag="VFX21_16410"
                     /inference="COORDINATES: protein motif:HMM:NF016826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEX5097608.1"
                     /translation="MIEPAGAAYRGATVALDVFTCEAGTDEVVRAPVADEAVLVVLEG
                     DCSFADRRVRRRSVFADRAAAVVLPPGASVAVHAHERTEFALASSVGGALPRVDGRPI
                     VVGPDEVVVHERGRAGWQRQVHDVVGEQVPARTLLVGETFNEPGQWSSYPPHKHD"
     gene            complement(469..933)
                     /locus_tag="VFX21_16415"
     CDS             complement(469..933)
                     /locus_tag="VFX21_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097609.1"
                     /translation="MTEYDDLLREAYLGEMFGDTFFATMAERQPDKARREKLETLQIV
                     EARTAQSLRRLIDRKGLSDAELDAREKGRELAEAIDPEDWDNFVSGLLGALPKFLADF
                     ERLRDIAKQPTDPALIALVNHEQAIEHFARLEVEGEEDKSLLPLQAHLRKPA"
     gene            complement(930..2168)
                     /locus_tag="VFX21_16420"
     CDS             complement(930..2168)
                     /locus_tag="VFX21_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5097610.1"
                     /translation="MASETIKLHWRKSETHGLEPDPEPRPIRYTLFSTDDHLVEPPGM
                     FDGRLPSALQDFAPKVVESEEGHEVWHFDGKVFFQVGLNAVVGRRREDWKVEPTRFDE
                     MRPGCYDVHARVKDMDINGMWASVNFPSQITGFCGSVFSRCSDPELGLAVTRAWNDWF
                     FDEWHAAYPDRIVPMGITYLGDAEHGAEEIRRNAARGFTAVTLPEMPHRIGMEPIFSD
                     WWDPIIAACAETNTVVCLHVGSTGVADMPKGAPTVPLGATLFGQLSLSACAEWLWSGY
                     AVRYPDLKIIMSEGGIGWVAMLHDRLENIVDRSGYGEYFPGDLRPAEVLHRNFWFSTI
                     DDPSTLSTRDVVGIDHITFESDYPHGDGTWPDTQAVFGEVFGGLRDDEIAKISHENAA
                     VLFRHPLPPPGNPHAIGVRT"
     gene            complement(2178..4469)
                     /locus_tag="VFX21_16425"
     CDS             complement(2178..4469)
                     /locus_tag="VFX21_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX5097611.1"
                     /translation="MDVGGLLAHMTLDEKLAQLGCVWSTQLVENDEFSESAARTHLVH
                     GIGEITRIGASTGLRPRERAQFANAIQRFCVEQTRLGIPTIVHEESTAGLCARDATQF
                     PQAIGLASTWNPALLERIGTVIREQMVATGARHTLAPVLDIARDPRWGRTEETYGESA
                     YLTSRLGVAYVRGVQGSLRDGVAATGKHFLGYGLSEGGMNHAPVHLGARELREVFAEP
                     FRAASHEAGLATVMNAYNSVDGLACGGSKAILDDLLRGELGFDGAVVADYFTTGLLIT
                     HHNVAATSGEAGRRALEAGLDMELPQLDCYGAPLKALIEAGAVDEALVDRSVRRVLAL
                     KEALGLFDEPYADEDAAEHVYFRAADAALAREAAQQSIVLLEHVDVLPLPAGTRIAVV
                     GPAADDERLLQGDYSYPAHTEIVWRQSGDAGILPQAGGAFAPGPYYPESVTPLAALRE
                     RFADVVYVRGCDLTERDDSGIHEAITAARDADVVVCCVGGRSGLLRECTSGEFIDATS
                     LELPGVQRTLVEAVLATGTPVVVVVMSGRVHSLPWLCDRAAALLYAWCPGEQGGAALA
                     DVLCGEVGPSGRLPISIPRTVGQVPVHHDHRAGGGRSQMLGDYSDAPSTPMYAFGFGL
                     TLTEFTYDNLVADAGTTADEITVRCRVTNDGDRDGTEVVQCYLRDETARCARPRMQLA
                     GFARVTLAPGDATTVTFRIDPTLLAYYDDTMQLVVEPGRVRVMVGGSATTADAYTTVI
                     VEGPERTIAPNDRRPTTVEYSRA"
     gene            complement(4578..5153)
                     /locus_tag="VFX21_16430"
     CDS             complement(4578..5153)
                     /locus_tag="VFX21_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX5097612.1"
                     /translation="MATTELQTATRPQPGTYEIDPSHSQVSFSARHLMVSKVRGSFAV
                     TGGSIVIAEDTEQSHVEATVDAQSVTSGDAKRDEHLRSADFFAVEQYPTISFRSTKLD
                     DHGDGTFTLDGDLTVRDVTHPVRLEGEYLGTETAPWGTTSIGFSAETEVNRKDWGVEW
                     NVALETGGVLVSEKVKLVIDVEAVVPQADAS"
     gene            5252..5746
                     /locus_tag="VFX21_16435"
     CDS             5252..5746
                     /locus_tag="VFX21_16435"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5097613.1"
                     /translation="MTEVARPAPQWLSEPEMQAWKALVATATGVLATLDRELQTEHGI
                     SLAEYEVLVHCSEQPEGSIRMSDLASAIHLSPSGMTRRIDAMVRRGLVRREACPTDRR
                     AFNVLLTDEGCTLLREAAPTHLRGVRAHFIDRLSPQQLTQLADAFSAIEIDPAAAAGG
                     CDRA"
     gene            complement(5773..>6160)
                     /locus_tag="VFX21_16440"
     CDS             complement(5773..>6160)
                     /locus_tag="VFX21_16440"
                     /inference="COORDINATES: protein motif:HMM:NF015424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX5097614.1"
                     /translation="HPASPYVAQLLGTNLLRGTAESAEQFRTENGFVLHVAIDGALRG
                     PSFAVIDPNAITLFTDAPHASARNTWRATVSDLDREPHRVRVRFSSPVELVAHVTPEA
                     AHELALAPGTDVWCAIKATAIAVHAL"
BASE COUNT          922 a         2114 c         2156 g          968 t
ORIGIN      
        1 cgtcgtgctt gtgcgggggg tacgacgacc attgcccggg ctcgttgaac gtctcgccga
       61 cgagcaacgt ccgcgcggga acctgttcgc cgacgacgtc gtgcacttgg cgttgccagc
      121 cggcgcggcc gcgttcgtgg acgacgacct cgtcgggtcc gacgacgatc ggccggccgt
      181 caaccctcgg caacgcaccg ccgacgctgg atgcgagcgc gaactccgtt cgctcgtgcg
      241 catgcacggc gaccgaagcg ccgggcggca ggacgaccgc ggctgcgcgg tccgcgaaga
      301 ccgaacgtcg gcgcacgcgc cggtcggcga aggaacaatc gccttcgagg acgacgagca
      361 ccgcttcgtc ggcgaccggg gcccgaacca cctcgtcggt tcccgcctcg catgtgaaca
      421 cgtcgagcgc gacggtcgcg ccgcgatacg ccgcgcccgc gggttcgatc atgcaggctt
      481 gcgcaggtgc gcctggagcg ggagcaacga tttgtcctcc tcgccttcga cctccaagcg
      541 ggcgaaatgc tcgatcgcct gttcgtggtt gacgagcgcg atgagcgcgg gatcggtcgg
      601 ctgcttcgcg atgtcgcgca gccgctcgaa gtcggcgagg aacttcggga gcgcgccgag
      661 caaaccgctg acgaagttgt cccagtcctc gggatcgatg gcctccgcga gctcgcggcc
      721 cttctccctc gcgtcgagct cggcgtcgct cagccctttg cgatcgatga gccggcgcag
      781 tgactgcgcg gtgcgcgcct cgacgatctg cagcgtctcc agcttctcgc gccgggcctt
      841 gtcgggctgg cgttcggcca tcgtcgcgaa gaacgtgtcg ccgaacatct cgccgaggta
      901 ggcctcgcgc agcaggtcgt cgtattcggt catgtgcgca ctccaatcgc gtgcgggttt
      961 cccggaggcg gcagcggatg ccggaacagc accgccgcgt tctcgtgact gatcttcgcg
     1021 atctcgtcgt cgcggagccc gccgaatacc tcgccgaaga cggcctgcgt atcgggccac
     1081 gtgccgtcac cgtgcggata gtcggactcg aaggtgatgt ggtcgatgcc cacgacgtcg
     1141 cgcgtgctga gcgtcgacgg gtcgtcgatc gtcgagaacc agaagttgcg gtgcagcacc
     1201 tccgccggcc gcaggtcgcc ggggaagtac tcgccgtagc cggaacggtc gacgatgttc
     1261 tcgagccggt cgtgcagcat cgcaacccac ccgatgccgc cttcgctcat gatgatcttc
     1321 aggtcggggt agcgcaccgc gtatcccgac cacaaccact ccgcgcacgc gctcaacgac
     1381 agctgcccga acaacgtcgc gccgagcggc accgtgggcg cgcccttcgg catgtcggcg
     1441 acgccggtcg aacccacgtg caggcacacg accgtgttcg tctccgcgca cgcggcgatg
     1501 atcggatccc accagtcgga gaagatcggc tccatgccga tgcggtgcgg catctcgggc
     1561 agggtcacgg cggtgaagcc gcgcgccgcg ttgcggcgga tctcctccgc gccgtgctcc
     1621 gcgtcgccga gatacgtgat gcccatcggc acgatgcggt cgggatacgc ggcatgccac
     1681 tcgtcgaaga accagtcgtt ccacgcacgc gtcaccgcga gcccgagctc gggatcggag
     1741 caacgggaga acaccgagcc acagaacccg gtgatctgcg acgggaagtt cacagacgcc
     1801 cacatgccgt tgatgtccat gtccttgacg cgggcgtgga cgtcgtaaca gccgggtcgc
     1861 atctcgtcga agcgcgtcgg ctcgaccttc cagtcctcgc gcctgcggcc gaccacggca
     1921 ttcaggccga cttggaagaa caccttgccg tcgaagtgcc agacctcgtg gccttcttcg
     1981 ctctcgacga ccttcggcgc gaagtcctgg agcgcactcg gcagccggcc gtcgaacatg
     2041 ccgggcggtt cgacgaggtg gtcgtcggtc gagaacagcg tgtagcggat gggccgcggc
     2101 tccggatccg gctcgaggcc gtgcgtctcg ctcttgcgcc agtggagctt gatcgtctcg
     2161 gacgccatgc cccgaatcta cgcccgggag tattcgaccg tcgtgggccg ccggtcgttg
     2221 ggcgcgatcg tccgttcggg cccttcgacg atgacggtcg tgtaggcgtc ggccgtcgtg
     2281 gcggagccgc cgaccatcac ccgcactcgg ccgggctcga cgacgagctg catcgtgtcg
     2341 tcgtagtacg cgaggagcgt cgggtcgata cggaatgtga ccgtcgtggc gtcgccgggc
     2401 gcgagcgtga cgcgcgcgaa gcccgcgagc tgcatccgcg ggcgtgcaca gcgcgccgtt
     2461 tcgtcgcgca ggtagcactg cacgacctcc gtgccgtcgc ggtcgccgtc gttggtgacc
     2521 cggcatcgca cggtgatctc gtcggcggtc gtcccggcgt cggcgacgag gttgtcgtag
     2581 gtgaattcgg tgagcgtcaa gccgaagccg aacgcgtaca taggcgtgga tggcgcgtcg
     2641 ctgtagtcgc cgagcatctg gctgcgtcca cccccggcgc ggtgatcgtg atggaccgga
     2701 acctgcccga cggtgcgcgg tatggagatg gggagccggc cactcggccc gacctctccg
     2761 cacagcacgt cggcaagggc ggcgccgcct tgctcccccg gacaccacgc gtacaggagc
     2821 gcggcggcgc gatcgcacaa ccacgggagc gagtgcacgc gcccgctcat cacgaccacg
     2881 acgacgggcg tgccggtcgc gagcacggcc tcgacgagtg tgcgttgcac accgggcagc
     2941 tcgaggctcg tcgcgtcgat gaactcgccg ctggtgcact cacgcagcag gcccgagcgg
     3001 ccgccgacac agcagacgac gacatcggcg tcgcgggctg cggtgatcgc ctcgtggatg
     3061 ccggagtcgt cgcgctcggt caggtcgcaa ccgcgcacgt acacgacgtc cgcgaagcgc
     3121 tcgcgcagcg ccgcgagcgg cgtcacgctc tcgggatagt acggaccggg cgcgaaggcg
     3181 ccgcctgcct gcggcaggat gcccgcgtcg cccgactgcc tccagacgat ctccgtgtgc
     3241 gcggggtacg agtagtcgcc ttgcaacaag cgctcgtcgt cggcggccgg accgaccacc
     3301 gcgatgcgcg tgccggccgg aagcgggagc acgtcgacgt gttcgagcag gacgatcgac
     3361 tgctgtgcgg cttcgcgggc caacgcggca tcggccgcgc ggaagtagac gtgttcggcc
     3421 gcgtcctcgt cggcgtacgg ctcgtcgaac aagcccagtg cttccttgag tgcgagcact
     3481 cgccgcaccg agcggtcgac gagcgcttcg tcgaccgcgc cggcttcgat cagcgccttc
     3541 agcggcgcgc cgtagcagtc gagctgcggg agctccatgt cgaggccggc ctcgagcgcc
     3601 cgccggcccg cctcgcccga tgtcgcggcc acgttgtggt gggtgatcag cagcccggtg
     3661 gtgaagtagt cggcgacgac ggcaccgtcg aagccgagct cgccgcgcaa caagtcgtcg
     3721 aggatcgcct tcgacccacc gcacgcgagg ccgtcgaccg agttgtacgc gttcatcacc
     3781 gtcgccagcc cggcttcgtg gctcgcagca cggaacggtt cggcgaacac ctcgcgcagc
     3841 tcacgcgcgc cgagatgcac gggcgcgtgg ttcatcccgc cctccgacag gccgtagccg
     3901 aggaagtgct tgccggtcgc ggcgacgccg tcgcgcaacg aaccctgcac gccgcgcacg
     3961 tacgcgacac cgagccggga cgtcaggtac gcggactccc cgtatgtctc ctcggtacga
     4021 ccccagcgcg gatcacgcgc gatgtcgagc accggtgcga gcgtgtggcg ggcaccggtg
     4081 gcgaccatct gctcgcggat gaccgtgccg atgcgttcga gcagcgccgg attccacgtc
     4141 gacgcgagac cgatcgcttg ggggaactgc gtcgcgtcac gtgcgcacag gcccgcggtg
     4201 ctctcctcgt ggacgatcgt cgggatcccc agccgggtct gctcgacgca gaaccgctga
     4261 atggcgttgg cgaattgcgc ccgttcgcgc ggccgtagtc ccgtcgacgc gccgatgcgc
     4321 gtgatctcgc cgatgccgtg cacgaggtgc gtgcgggccg cggactcgga gaattcgtcg
     4381 ttctcgacga gctgcgtcga ccacacgcag ccgagctgcg cgagcttctc gtcgagcgtc
     4441 atgtgcgcga gcagtccgcc gacatccatg ccggcggacc gtactcgcag ccattgtgtt
     4501 gtgcgttacg tgcggacgca gccgcgtcct tcccgcggcg aagccgcccc tcggatcgca
     4561 gcggagggag ccatgggcta cgacgcgtcc gcttgcggca ccacggcctc gacgtcgatg
     4621 acgagcttca ccttctcgct gacgagcacg ccaccggtct cgagcgcgac gttccactcg
     4681 acgccccagt ccttgcggtt cacttcggtc tccgcggaga agccgatgct cgtcgtgccc
     4741 cacggcgcgg tctcggtacc gaggtactcg ccctcgagtc gtaccgggtg cgtgacgtca
     4801 cggacggtca ggtcgccatc gagcgtgaac gtgccgtcgc cgtggtcgtc caacttggtc
     4861 gaacggaagg agatcgtcgg gtactgctcg accgcgaaga agtcggcgga ccgcaggtgc
     4921 tcgtcgcgct tggcgtcacc ggaggtgacc gactgcgcgt cgacggtcgc ttcgacgtgc
     4981 gactgctcgg tgtcttccgc gatgacgatg ctgccgcccg tgaccgcgaa cgacccgcgg
     5041 accttcgaga ccatcaggtg ccgggcgctg aacgagacct ggctgtgcga ggggtcgatc
     5101 tcgtaggtgc cgggctgggg gcgggttgcg gtctggagct cggtggtggc cacggtggcc
     5161 tccttcgggg gtagttgcga gtacaaaact tgatgacgca agtaactata ccacagatat
     5221 ttgcgtatgc aagtagttgt acaatcgggc catgaccgag gtcgcccggc cggcgcccca
     5281 gtggctctcg gagccggaga tgcaggcgtg gaaggcgctg gtcgccaccg cgaccggggt
     5341 cctggccacg ctcgaccggg agctccagac cgagcacggc atctcgctgg ccgagtacga
     5401 ggtgctcgtc cactgctcgg agcagcccga gggctccatc cgcatgagcg acctcgcgtc
     5461 ggcgatccac ctctcgccga gcggcatgac gcgccgcatc gacgccatgg tgcggcgcgg
     5521 cctcgtgcgc cgggaggcgt gcccgaccga tcggcgcgcg ttcaacgtgc tgctcaccga
     5581 cgaaggctgc acgcttctgc gggaggccgc gccgacgcac ctgcgcggcg tgcgcgccca
     5641 cttcatcgac cgcctctcac cgcagcagct cacgcaactc gcggacgcgt tctcggccat
     5701 cgagatcgac cccgccgccg cagccggcgg ctgcgaccgc gcctagcgca ccggcgcacg
     5761 cgcctgcgtg ggtcagagcg cgtggacggc gatggccgtt gccttgatcg cacaccacac
     5821 gtcggtgccg ggggcgagcg cgagctcgtg cgcggcctcg ggcgtcacgt gggcgacgag
     5881 ctcgaccggt gacgagaacc gcacgcggac gcgatggggt tcacggtcga ggtcgctgac
     5941 ggtcgcgcgc cacgtgttgc gggcactggc gtgcggcgcg tccgtgaaca acgtgatggc
     6001 gttcggatcg atgaccgcga acgacggtcc ccgcagcgcg ccgtcgatcg cgacgtgcag
     6061 gacgaatccg ttttcggtgc ggaactgctc cgcggactcg gcggtgccgc gcagcaagtt
     6121 ggtgccgagg agctgggcga cgtagggcga cgcggggtgt
//
LOCUS       DASUGA010000832         2315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1768890, whole genome shotgun sequence.
ACCESSION   DASUGA010000832 DASUGA010000000
VERSION     DASUGA010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1768890"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VFX21_16445"
     CDS             <1..359
                     /locus_tag="VFX21_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HEX5097615.1"
                     /translation="GLDHRADPHYPGVRDLTLSPSTTLAGRKATADKGVEVAELTAVV
                     SADELILADALGLGSDVAINPSGGALVSNPTMVTGLARIGEAAQQVMDGKQRALGHAS
                     SGPCLQQNLVCVVEAR"
     gene            361..1506
                     /locus_tag="VFX21_16450"
     CDS             361..1506
                     /locus_tag="VFX21_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HEX5097616.1"
                     /translation="MAERCAVVGVGMTKMSKCRDDVSMAGLLRQAAQRALEDAEMTWK
                     DVEAVVIGKAPDIFEGVMMPELYLADALGAAGKPIMRVHTAGSVGGSTAIVAAHLIES
                     GVHERVLTVAFEKQSEGDTNFAFSAGRSGGVGAGGYFAPHVRAYIERSGAPDYVGPMV
                     AVKDRQNALKNPYAHLQIKDISIEMVLESPFVWAPIHRLESCPTSDGACAMVLTNEAG
                     GRRASKPPAWVHGTAVRTELGWFPGRDPVNPQGGQDCAADVYKQAGITNPREELDVAE
                     IYVPFSWYEPMWMENLGFAAVGEGWKMTESGATSMEGDMPINASGGVLSSNPIGASGM
                     LRFAEAALQVRGLAGEHQVENARKALGHAYGGAAQYFSMWIVGSEMR"
     gene            1507..>2315
                     /locus_tag="VFX21_16455"
     CDS             1507..>2315
                     /locus_tag="VFX21_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5097617.1"
                     /translation="MDVDLPAEDDPRRLEVRQWFADHPQPSPKDLVDAGFVVPHWPKP
                     YGLDADPALQLIIEDEMRRAGAVKPTNPIGIGHCGPILVALASDEQKQRYLWPMLTGE
                     EMWCQLFSEPGAGSDLANLSTRAERDGDVYVVNGQKIWTSLADVAKFGILIARTRADV
                     SKHRGISYFIIPMDLPGIEIRPIRNMTGGAGFNEVFFTDVRVPVENIIGEENLGWGMA
                     KTTLANERVSLSTGGGLQWGYGPSARDLVTLMKGRAPNAVQRQEIVDAYIEG"
BASE COUNT          400 a          743 c          828 g          344 t
ORIGIN      
        1 cgggcctcga ccaccgcgcc gatccgcact acccgggcgt acgggacctg acgttgtcgc
       61 cgtcgaccac gctcgcggga aggaaggcca ccgcggacaa gggcgtcgag gtcgcggagc
      121 tcacggcggt cgtctcggcg gacgagctga tcctcgccga cgcgctcggg ctcgggagcg
      181 atgtcgcgat caacccgtcg gggggcgcgc tcgtcagcaa cccgacgatg gtcaccggtc
      241 tcgcgcgcat cggtgaagcc gcgcagcaag tgatggatgg caagcagcgg gcgctcgggc
      301 acgcaagcag tggcccatgc ctgcaacaga acctcgtctg cgtcgtggag gctcgctgag
      361 atggcggaaa ggtgtgcggt cgtcggcgtc ggcatgacga agatgtcgaa gtgccgcgac
      421 gacgtgtcga tggcaggcct gctgcgccaa gccgcgcagc gtgcgctcga agacgcggag
      481 atgacgtgga aggacgtcga ggccgtcgtc atcggcaagg cacccgacat cttcgaaggc
      541 gtcatgatgc ccgagctcta cctggcggac gcgttgggcg ccgcgggcaa gccgatcatg
      601 cgcgtgcaca ccgcgggcag cgtcggcggt tcgaccgcga tcgtcgccgc gcacttgatc
      661 gagtcgggtg tgcacgaacg tgtgctcacg gtcgcgttcg agaagcagtc cgagggcgac
      721 accaacttcg cgttctcggc cggccgcagt ggcggtgtcg gcgcgggcgg ctacttcgcg
      781 ccgcatgtgc gcgcgtacat cgaacggtcg ggcgcacccg actacgtcgg ccccatggtc
      841 gcagtgaagg accggcagaa cgcgctcaag aacccgtacg cgcacttgca gatcaaggac
      901 atctcgatcg agatggtgct ggagtcaccg ttcgtctggg cgccgatcca ccggttggag
      961 tcgtgcccga catcggacgg cgcgtgcgcg atggtgctca ccaacgaagc gggtggcagg
     1021 cgcgcgtcga agccgccggc gtgggtgcac ggcaccgcgg tacgcaccga gctcggctgg
     1081 ttcccgggcc gtgaccccgt gaatccccag ggcgggcagg actgtgcggc cgacgtctac
     1141 aagcaggccg gcatcacgaa cccgcgcgag gagctcgacg tcgcggagat ctacgtgccg
     1201 ttcagttggt acgagccgat gtggatggag aacctcgggt tcgccgcggt cggtgagggt
     1261 tggaagatga ccgagagcgg cgcgacgtcg atggaaggcg acatgccgat caacgcgagc
     1321 ggcggcgtgc tgtcgtcgaa cccgatcggt gcgtcgggca tgctgcgttt cgcggaagcg
     1381 gcgttgcagg tgcgcggcct cgccggtgag caccaagtcg agaacgctcg caaggcgctc
     1441 ggtcacgcgt acggcggcgc cgcgcagtac ttctcgatgt ggatcgtcgg ctcggagatg
     1501 cgctaagtgg acgtggatct cccggcggag gacgatccac gtcggctcga agtacgccag
     1561 tggttcgcgg accatccgca accgtcgccc aaggacctcg tcgacgcggg cttcgtcgtg
     1621 ccgcactggc ccaagcccta cgggctcgac gccgatcccg cgttacagct catcatcgag
     1681 gacgagatgc ggcgcgcggg cgcggtcaag cccacgaacc cgatcggcat cgggcactgc
     1741 ggaccgatcc tcgtcgcact ggccagtgac gagcagaagc agcgctacct ctggccgatg
     1801 ctcaccggtg aagagatgtg gtgccagctg ttcagcgagc cgggcgccgg ctccgacctc
     1861 gcaaacctga gcacgcgcgc cgagcgcgac ggcgacgtct acgtcgtgaa cggccagaag
     1921 atctggacgt cgctcgccga cgtcgcgaag ttcggcatcc tcattgcgcg gacgcgtgcc
     1981 gacgtgtcga agcaccgcgg catctcgtac ttcatcatcc cgatggacct gcccggcatc
     2041 gagatccgcc cgatccgcaa catgaccggc ggcgcgggct tcaacgaagt gttcttcacc
     2101 gacgtgcgcg tacccgtgga gaacatcatc ggcgaggaga acctcggctg gggcatggcg
     2161 aagacgacgc tcgccaacga acgcgtgtct ctgtcgaccg gtggtggtct ccagtggggc
     2221 tacggaccga gcgcgcgcga cctggtgacc ctgatgaagg gccgcgcacc gaacgcggtg
     2281 cagcgccagg agatcgtcga cgcgtacatc gaagg
//
LOCUS       DASUGA010000833         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_656614, whole genome shotgun sequence.
ACCESSION   DASUGA010000833 DASUGA010000000
VERSION     DASUGA010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656614"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1347)
                     /locus_tag="VFX21_16460"
     CDS             complement(154..1347)
                     /locus_tag="VFX21_16460"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX5097618.1"
                     /translation="MTDGDSPKIEDAPTPGLFEAWQLPEGSDSDERAESAARKNVPWW
                     GWVLLGFSILLTAVVIAGFVIRVDYFTIAPGEAVTLNGLVKIDGAKSFPDKRGDIKLL
                     FVRERNHVNLWRYLQAKLGSDIDLYKEEQLNPDNKSQTQLNNQAIQQMADAKTAATKV
                     ALESAGFKVGQAPGLTVSDLITGFPAEKVLELGDVILSANGSKITRSQTLGDIVTKLK
                     AGDKVTLEIERDGKKQTVEVPVRVDPQSGRTLIGVFASPRFEFPVSVDVDTSSIGGPS
                     AGLAMSLAILDDLTPGNLTGGKHVAVTGTIDPDGRVGEIGGISQKAVAARAAGAQLFL
                     VPQCTSDNADALQSCKADLARATERAGKNVKVIPVATMADALRALRANGGEAVEQVGA
                     SDRAA"
     gene            1417..1623
                     /locus_tag="VFX21_16465"
     CDS             1417..1623
                     /locus_tag="VFX21_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097619.1"
                     /translation="MSIAHQSAMPTVRPKARQRGKLHPVPTRRPLFALAKVGGLLALT
                     AFGAALAAGTVGLGLVMVLTSVAG"
     gene            1736..>2241
                     /locus_tag="VFX21_16470"
     CDS             1736..>2241
                     /locus_tag="VFX21_16470"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX5097620.1"
                     /translation="MIDPTERAANAATERDVDQLELLVRDDDARVRAIALAALVRVAP
                     APTAATTWVAAAHDADPRVRRRAAEVAPKLGRSARTRTLSELLADDDAWVAEAAAYAI
                     GEHPRPTATAIDALRAAATSHTDALVREAAVAALGAIGDPSTLPAVLAACDDKPAIRR
                     RAVLALAA"
BASE COUNT          348 a          809 c          728 g          356 t
ORIGIN      
        1 gtgatcacgc gctgccgtcg atcgttctca tgcatcggga atccccccag acaccccgca
       61 ccggggtcgg cgatcctccg cctggcggca tgctaaccgc tcacccgcgc gaggcgcctg
      121 gagcgggctt ccagcaatca gcccagtgat cggttacgcg gcgcgatcag acgctccgac
      181 ttgttcaacc gcctcaccgc cgttcgcgcg caacgcccgt aacgcgtctg ccatcgtcgc
      241 gaccggaatc accttcacgt tcttgcccgc ccgctcggtt gcgcgcgcga gatcggcctt
      301 gcacgactgc agcgcgtcgg cgttgtcact cgtgcactgc ggaacgagga acagctgcgc
      361 gccggccgcg cgcgcggcga ccgccttctg gctgatgccg ccgatctcgc cgacgcgacc
      421 gtcgggatca atggtgccgg tgactgcaac atgcttgccg ccggtgaggt tgccgggcgt
      481 gaggtcgtcg agaatcgcga gcgacattgc gagcccggcc gacggaccac cgatactcga
      541 cgtgtcgacg tccaccgaaa ccgggaactc gaaacgcggc gaagcgaaca cgccgatcag
      601 cgtccggccg gactgcggat cgacccggac cggaacttcg acggtctgct tctttccatc
      661 ccgctcgatc tcgagcgtga ccttgtcacc cgccttcagc ttggtcacga tgtcgccgag
      721 cgtctggctg cgcgtgatct tcgacccgtt cgcgctgagg atgacatcgc cgagctcgag
      781 gaccttctcg gccgggaatc cggtgatgag atcgctcacg gtcagccccg gcgcctgccc
      841 gaccttgaag cccgccgatt cgagtgcgac cttcgtcgcc gcggtcttgg catcggccat
      901 ctgttgaatc gcctggttgt tcagctgcgt ctgcgacttg ttgtcggggt tcagctgctc
      961 ctctttgtag aggtcgatgt cgctgccgag cttcgcctgc aagtagcgcc acaagttgac
     1021 gtggttccgc tcccgcacga acaagagctt gatgtcgccg cgcttgtcgg ggaacgactt
     1081 ggccccgtcg atcttcacca agccgttcaa cgtgaccgct tcaccgggcg cgatcgtgaa
     1141 gtagtcgacg cggatgacga accccgcgat cacgaccgcg gtcaacaaga tcgagaaccc
     1201 gagcaggacc cagccccacc agggcacgtt cttgcgcgct gccgactccg cccgctcatc
     1261 ggagtcggac ccctccggca gttgccacgc ctcgaacaag ccgggcgtgg gcgcgtcttc
     1321 gattttcgga gagtcgccgt cggtcatttg cttcagctct ggggacgaga cgccgaccga
     1381 agagggaagc agtccgcgag aagttggagc ggcgtcatgt ccatcgccca ccagagtgcc
     1441 atgccgacgg tccgtccgaa ggcgcggcaa cgcggcaagc tccaccccgt tccgacccgc
     1501 cggccgctct tcgcgctggc caaggtcggg ggcctgctcg cactgacggc gttcggcgca
     1561 gccctcgccg ccggcaccgt gggcctcggc ctcgtgatgg tgctcaccag cgtcgcgggc
     1621 tgacgcccgg gagccccgca tcgccggtgc cgccccgtcc aacggcaccg acgcgcaaag
     1681 ttgcaaccgc atcggcaccg cagtgcgccg gctgtaggga ataccgtccc gaaccgtgat
     1741 cgacccgacg gaacgcgcgg cgaatgcggc gaccgaacgc gacgtcgacc aactcgagct
     1801 gctggtgcgc gatgacgacg cgcgggtgcg tgccatcgcc ctggccgcgc tcgtccgggt
     1861 cgcgccggcg cccaccgcgg cgacaacatg ggtcgcagcc gcgcacgacg cagacccgcg
     1921 tgtacgccgg cgggcggccg aagtcgcacc caaactcggg cggtcggcgc gcacccgcac
     1981 gctgagcgag ctgctcgccg acgacgacgc gtgggtcgcg gaagcggccg cgtacgcgat
     2041 cggtgaacac ccgcggccga ccgcaactgc gatcgacgca ctccgcgccg ccgcgacatc
     2101 acatacggac gcgctcgtgc gcgaggcggc ggttgcggcg ctcggcgcga tcggcgaccc
     2161 gtcgacgttg ccggcggtgc tggccgcgtg cgacgacaag cctgcgattc gaagacgcgc
     2221 ggtccttgcc ctcgccgcct t
//
LOCUS       DASUGA010000834         3996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1351850, whole genome shotgun sequence.
ACCESSION   DASUGA010000834 DASUGA010000000
VERSION     DASUGA010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351850"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VFX21_16475"
     CDS             <1..934
                     /locus_tag="VFX21_16475"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX5097621.1"
                     /translation="TRRYAVNGVFWDGVDGYRGSYLTAYNNGDYGIYAFASVSGQIDH
                     SYASGSPDSGFYIGQCDPCKAVITDVVSENSQLGYSGTNASDVTIVNSVWRKNRIGIV
                     PNSQDVEELPPVKRNTIVGNVVYANDNLEAATAKNPLYDALVGSGIAMTGTHDSLVAR
                     NRVYDHSWFGIVLAPFPSDRTLYTVERNKVIGNVVEGSGHADLALVFSSPAQRNCFSG
                     NTFKTSAPNRIEQRLPCTGTGIGGDLSPGAVSPDELLNRDEPEGKPFSEQPTPPEQQN
                     MPNASDAKPLPARANVVPVKIDVDAIRVPARANP"
     gene            990..1451
                     /locus_tag="VFX21_16480"
     CDS             990..1451
                     /locus_tag="VFX21_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX5097622.1"
                     /translation="MSYTVDFKTVSTVGLESSPVADALAGLRANEARYFKNKYDHTYV
                     VEPASKAKKTIDYVARILKNERDIVIASKPLEATSFEVEGTRWAYVFYESGLSINVMY
                     GIEEGGKRAVGFKLSEGMDVPEELSSFKFARQKSKLAGTIRGSYFVIKQEY"
     gene            complement(1435..2505)
                     /locus_tag="VFX21_16485"
     CDS             complement(1435..2505)
                     /locus_tag="VFX21_16485"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX5097623.1"
                     /translation="MAAGERGEQHRGPSTRAFWVVAVLLVFASSGCDAASSKSASPSS
                     SPSTTTPAPSATGPRVIIDTDLSLWWDDATAIGIANVLQQQGAANVLGIMADVKNPVA
                     VAAIDAINPAYGHDDIPLGAVANSKADTFRHGYTDVVTQRLPHSVGGSDDVPEAVALY
                     RELLGQQPDRSVTIVSLGAYTNLAGLLAAPDGPALVSAKVKRLVIMDGIFPTGMGPVT
                     NQKLDLAAARAVIAGGPDAAPWPTPIAWVDGVPGIGTRVGETLCEKTATDHPMRIVYE
                     ALFKCGKVTDGNWDAPALLFALGDIPEVFSVEGRGGAAAINAQGGLSWQPTSSRRDDF
                     YVHLRDQKRLNERIDQLLNTPA"
     gene            2622..3647
                     /locus_tag="VFX21_16490"
     CDS             2622..3647
                     /locus_tag="VFX21_16490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097624.1"
                     /translation="MRQLAIAALAVSVVMVGSTSAGAAPNSSTPNNQTPRQSEHDRIT
                     AFWTNERVAQAIPRDFDYDAHTRSFKPSARPAPGGGGSGTPSTTVGSSWTAGGEVLQT
                     TGKVLFAMAGVYYVCSASVANDTVSTRSIILTAGHCVYDETNKAFATNWMFVPDYDSA
                     PATLTTSGSFCAQTKLGCWTANALVAAGGFANAGSFNDQAIVHDYAFAVVGSGGKSGS
                     AQLDTTVGGHPLQFSALSAGADTSLFGYPAAGKYKGKDLVYCRGPLGFDSTVGNATYR
                     VACNMTGGSSGGPWFSPFSNGSGTMVSVNSYGYSGTTAMFGPKLNSETASMFAAAATA
                     NANTIVP"
     gene            3681..>3996
                     /locus_tag="VFX21_16495"
     CDS             3681..>3996
                     /locus_tag="VFX21_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003934699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5097625.1"
                     /translation="MARNLNDTPVALVTGASRGIGRAGALALAEAGFDVVVSARTVKE
                     GDGRSEARSVREARPSAAVPGSLERTAAEIEARGRRALIVPMDLTDADSVRGLPPAVL
                     DEW"
BASE COUNT          725 a         1291 c         1302 g          678 t
ORIGIN      
        1 cacgcgccgc tatgcggtca acggtgtgtt ctgggacggc gtcgatggat atcgcgggtc
       61 gtatctcacg gcgtacaaca acggcgacta cggcatctac gcgttcgcga gtgtcagtgg
      121 acagatcgat cacagctacg cgtccgggag tcccgactcg ggcttctaca taggccagtg
      181 tgatccatgt aaggcggtca tcactgacgt cgtctccgag aacagtcagc ttggctactc
      241 ggggacgaac gcgagtgacg tcacaatcgt gaactcggtg tggcgcaaga accgcatcgg
      301 catcgtcccc aacagccaag acgtcgagga gcttccaccg gtcaaaagga acacgatcgt
      361 cggaaatgtc gtctacgcca acgacaatct cgaggctgcc accgcgaaga acccgttgta
      421 cgacgccctc gttggttccg gcatcgccat gacggggacc catgacagtc tcgtcgcgcg
      481 caaccgcgta tacgaccact cctggttcgg catcgtgctc gcgccgttcc cgtccgatcg
      541 gacgttgtac accgtcgaac gcaacaaggt gatcggcaat gtcgtcgagg gcagcggcca
      601 cgccgacctc gcgctcgtgt tctcctcacc cgcgcagcgc aactgcttct ccggtaacac
      661 gttcaagacc tcggcgccaa accgcatcga gcagcgcttg ccctgcacgg gaactggcat
      721 cggcggcgat ctctctcccg gcgcagtgag tcccgacgaa ctgttgaatc gggacgagcc
      781 ggagggcaaa cccttctccg aacaaccgac accacccgag cagcagaaca tgccgaacgc
      841 ttccgacgcg aagccgctac cggcccgcgc gaatgtcgta ccggtcaaga tcgacgtcga
      901 tgcgatccgt gttccggcgc gggcgaatcc gtaactcgcg ccaaacgcta gcgttcgaac
      961 accaagatcc gaagccgttg gagtgagccg tgtcctacac cgtggatttc aaaaccgtgt
     1021 cgacggtcgg gttggagtcg tcccccgtcg cggacgcgct cgcagggttg cgcgcgaacg
     1081 aagcccgcta cttcaagaac aagtacgacc acacctatgt cgtcgagccg gcgagcaagg
     1141 ccaagaagac gatcgactac gtcgcccgga tcttgaagaa cgaacgcgac atcgtcatcg
     1201 cgtcgaagcc tctcgaggca acctccttcg aggtcgaggg aacccggtgg gcatacgtgt
     1261 tctacgaaag tggcctgtcg atcaacgtga tgtatggcat cgaggaaggc gggaagcgag
     1321 cggtcggctt caagctgtcg gaaggcatgg acgttccgga ggaactttcg tccttcaagt
     1381 tcgcgcgcca gaaatcaaag ctcgccggaa cgatccgcgg ttcctacttc gtgatcaagc
     1441 aggagtattg agcagctgat cgatgcgctc gttcagccgc ttctgatcgc gcaggtgcac
     1501 gtaaaagtcg tcgcggcgcg acgacgtcgg ttgccacgac aagccgccct gcgcgttgat
     1561 cgccgctgcg ccgccacgcc cttcgacgga aaagacctct ggtatgtccc ccagcgcgaa
     1621 gagcagcgcg ggggcgtccc agttgccgtc ggtcaccttc ccacacttga acaacgcctc
     1681 gtacacgatt cgcatcgggt ggtcggtcgc cgtcttctcg cacagtgtct cgccgactcg
     1741 cgtgccaatt ccggggacgc cgtcgaccca cgcgatcggg gtgggccacg gagctgcatc
     1801 cgggccgccc gcgatgaccg cacgcgcggc agccaggtcg agcttctggt tggtcaccgg
     1861 tcccatgccg gtcgggaaaa tgccgtccat gatcacgagg cgcttgacct tcgcgctcac
     1921 caaggcgggc ccgtcgggtg cggccagcag tccggcgaga ttcgtgtacg caccgagcga
     1981 cacgatggtg acactgcggt cgggctgctg accgagcagc tcccggtaca acgcgaccgc
     2041 ttcgggtacg tcgtcgctgc cgccaaccga gtgcgggagt cgctgcgtga cgacgtcggt
     2101 gtagccgtga cggaacgtgt cggccttgct gttcgcgacc gcacccaacg gaatgtcatc
     2161 gtggccgtac gcggggttga tggcgtcgat ggcggcgacc gcgacgggat tcttgacgtc
     2221 ggccatgatg ccgaggacgt tggccgcgcc ttgctgctgc agcacgttcg cgatgccgat
     2281 cgcggtggcg tcgtcccacc agagcgacag atcggtgtcg atgatgacgc gcgggccagt
     2341 tgcactcggc gcgggcgtcg tggtcgacgg ggaggaactg ggcgaggccg acttcgacga
     2401 agcggcgtca cagccgctgg atgcgaacac gagcaacacg gccaccaccc aaaacgcgcg
     2461 ggtcgatggg ccgcggtgct gctcacctcg ttcgccggcc gccatcgcag ctcctcgtgg
     2521 gtggtcaacg cgttgtctga agcgcccttt tgaccctaga ttggtcaccg agcgtgtgct
     2581 cggaaacgca tcatgtgaag gcaggggtgg aagtgcgggg aatgcgacag ctggcgatcg
     2641 cggcgttggc cgtgtccgtc gtcatggtgg gctcaaccag cgctggggcg gcgccgaaca
     2701 gcagcacgcc gaacaaccaa acgcctcgcc agtccgagca cgatcggatc accgcgttct
     2761 ggaccaacga gcgtgttgcg caggcgatcc cgcgcgactt cgactacgac gcgcacactc
     2821 ggtcgttcaa gccgagcgcg cggccggcgc ccggtggggg cggtagtggg acaccctcga
     2881 ccaccgtcgg ttcgtcgtgg accgccggtg gagaagtgct ccagacaacc ggcaaggtgt
     2941 tgttcgcgat ggccggcgtc tactacgtgt gctccgccag cgtggcgaac gacacggtga
     3001 gcacgcggtc gatcatcctg acggccgggc actgcgtgta cgacgagacg aacaaggcgt
     3061 tcgcgaccaa ctggatgttc gttcccgact acgacagcgc gcccgcgacg ctgacaacgt
     3121 cgggatcgtt ctgtgcgcag acgaagctcg gttgctggac cgcgaacgcg ctcgttgctg
     3181 cgggcgggtt tgcgaacgcg ggaagcttca acgaccaagc gatcgtccac gactacgcat
     3241 tcgccgttgt cggcagtggc ggcaagagcg gatccgcaca gctcgacacg accgttgggg
     3301 gtcacccgtt gcagttctcg gcgctgagtg ccggcgccga cacgtcgctg ttcggctacc
     3361 cggcggccgg caagtacaag ggcaaggacc tcgtgtactg ccgcggtccg ctcggtttcg
     3421 actccaccgt tggcaacgcg acctaccgcg tcgcttgcaa catgacgggc ggatcgtccg
     3481 gcggcccctg gttctccccg ttcagcaacg gttcgggaac gatggtttcc gtgaactcct
     3541 acggctacag cggcacgacc gcgatgttcg gccccaagct gaactcggag acggcgtcga
     3601 tgtttgctgc tgccgccact gcgaacgcga acacgatcgt tccctgagcc ttcggtcacg
     3661 cgttcgagta cgttgcgcgg gtggcgcgca acttgaatga cacaccggtt gcgctggtca
     3721 ccggtgccag ccgtgggatc ggacgcgccg gcgcgctcgc gctcgccgaa gccggattcg
     3781 acgtggtcgt gagcgcccgc acggtcaagg aaggcgacgg ccggtccgaa gcgcgttcgg
     3841 ttcgagaagc gcgcccctcg gccgcggtgc ccggcagcct cgaacgcact gccgccgaga
     3901 tcgaagcgcg cggccgccgc gcgctgatcg tgccgatgga tctcaccgat gccgattcgg
     3961 tgcgaggcct tccacccgcc gtgctggacg agtggg
//
LOCUS       DASUGA010000835         5438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_137710, whole genome shotgun sequence.
ACCESSION   DASUGA010000835 DASUGA010000000
VERSION     DASUGA010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137710"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2388)
                     /gene="rpoC"
                     /locus_tag="VFX21_16500"
     CDS             complement(<1..2388)
                     /gene="rpoC"
                     /locus_tag="VFX21_16500"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX5097626.1"
                     /translation="MFDVNESHRLTIHLATAGDILTWSNGEVKKPETINYRTLKPEKD
                     GLFCEKIFGPTKDWECYCGKYKRVRFKGIICERCGVEVTRSKVRRERMGHIQLAAPVT
                     HIWYFKGVPSRLGYLLDIAPKSLEKVIYFAAHLITWVDVEKLHTDLPSLEAEMRAEID
                     ELEREREVKLRDRDETYIKELEEIEGRGGKKAETERAEKAKNKDFEEIRLRYEEEIGQ
                     LQLVWDTLKSLKPKQLIDDERVWRELTDRYHEYFAGGMGAEAVKDLVSRLDLNEVETD
                     LKETIATAKGQRKAKAIKRLKVVSAFNRRDDGGRVVNSPMGMILDVVPVIPPDLRPMV
                     QLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPEIIINNEKRMLQEAVDALFDNGR
                     RGRPVTGPGNRALKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPQLRLQQCGLP
                     KQMALELFKPFVMKRLVDLEYAQNIKSAKRMVERARPQVWDVLEEVIKEHPVFLNRAP
                     TLHRLGIQAFEPVLVEGKAIQIHPLVCAAFNADFDGDQMAVHLPLSAEAQAEARLLML
                     SAHNVLSPASGRPIAVPSQDMIIGHYYLTEMIDDGKGHGRVFTSLDEAFAAYEERYGG
                     DGETVGLHSKIKVRMPVGKFPADVFPRPLDDGTAQSIVLREYVASASPNGASTNGSTE
                     VLVETTLGRLLFNSAFPDDFPFVDRVMKKRDVTEVVGELVVGYDKAVVANSLDALKNL
                     GFEWSTRSGLTISVADVKTPDAKAGLLEQFEGEAAKVENQFERGIITDDERRQQEI"
     gene            complement(2407..>5438)
                     /gene="rpoB"
                     /locus_tag="VFX21_16505"
     CDS             complement(2407..>5438)
                     /gene="rpoB"
                     /locus_tag="VFX21_16505"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018638481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX5097627.1"
                     /translation="EKDVVDAKMIPGRGAWLEFEVDKKDIAYVRIDRKRKQPVTILLK
                     ALGFGESDDELLNLITDEVGRPYESMRNTLDKDNTTTVDEAFIDIYRKLRPGEPPTPD
                     SARALLENLFFNSKRYDQAKVGRHKVSKKLSREYPLLGLERFDLDIPDANVENRSGDF
                     TLTKADILATVSYLVKLHNRDAGYYPDDIDHFGNRRVRTVGELIQNQLRVGLSRMERV
                     VRERMTTQDVEAITPQTLINIRPVVAAIKEFFGSSQLSQFMDQTNPLAGLTHKRRLSA
                     LGPGGLSRERAGFEVRDVHPSHYGRMCPIETPEGPNIGLIGYLSTFARINRFGFIETP
                     YRKVEKGRVTDEIAYLAADDEDEYVIAQANARVDEQGNFLDDRVLVRAGRGEIAYVTA
                     HEVDYMDVSPKQLVSVATALIPFLEHDDANRALMGANMQRQAVPLLRATAPYIGTGVE
                     ARAARDAGDVLLAEDDGTVVDVAGDTITVDYAKKGRTAHRLWKFRRSNQGTCINQKPV
                     VSEGVQVKKGDVLADGPSTHLGELALGKNLLVAFMPWKGHNFEDAIVLSERLVKDDVL
                     TSIHIEEHEIDARDTKLGAEEITRDIPNLSEDILTDLDERGIIRIGAEVGPGDILVGK
                     VTPKGETELTPEERLLRAIFGEKAREVRDTSLKVPHGENGKVIDVRVFSREDGHELPP
                     GVNQLVRVYVGQKRKISEGDKLAGRHGNKGVIAKILPVEDMPYLADGTPVDIVLNPLG
                     VPSRMNVGQVLESHLGYVARHGWEGQEAKPTEPHKTRTVTEPAVWISSPVFDGAHWDE
                     TEETGDHVKTIQELFDHLHPDGADGQRMIGRDGKATLHDGRTGQAYDRPVTVGYMYIL
                     KLSHLVDDKIHARSTGPYSMITQQPLGGKAQFGGQRFGEMEVWALEAYGSAYALQEIL
                     TIKSDDVLGRVKVYEAIVKGENIPEPGIPESFKVLIKEMQALCLNVRVMNENGEQIEI
                     RELDEDAFRTTESLGIDLSRPERGTDEEDAATARSRF"
BASE COUNT          790 a         1768 c         1824 g         1056 t
ORIGIN      
        1 gatctcctgc tggcgccgct cgtcgtcggt gatgatgccg cgctcgaact ggttctcgac
       61 cttggccgct tcaccttcga actgctcgag cagtccggcc ttggcgtcgg gcgtcttcac
      121 gtccgcgacc gagatcgtca gacccgaacg ggtcgaccac tcgaagccga ggttcttcag
      181 cgcgtcgagg ctgttggcca cgaccgcctt gtcgtagccg acgaccagct cgccgacgac
      241 ttcggtgacg tcacgcttct tcatcacgcg gtcgacgaac gggaagtcgt ccgggaacgc
      301 gctgttgaac agcagccggc cgagcgtcgt ctcgacgagc acctcggtcg agccgttggt
      361 gcttgcgcca tttggcgaag ccgaggcgac gtactcgcgc agcacgatcg actgcgccgt
      421 gccgtcgtcg agcgggcgcg ggaacacgtc cgccgggaac ttgccgaccg gcatgcgcac
      481 cttgatcttg ctgtgcagac cgacggtctc gccgtcgcca ccgtagcgct cctcgtacgc
      541 ggcgaacgcc tcgtcgagcg acgtgaacac gcggccgtgg cccttgccgt cgtcgatcat
      601 ctcggtcagg tagtagtgac cgatgatcat gtcctggctc ggcaccgcga tcgggcggcc
      661 cgaggccggc gacaacacgt tgtgcgcact cagcatgagc aggcgcgctt cggcctgcgc
      721 ctccgcggag agcgggaggt gcacggccat ctggtcaccg tcgaagtccg cgttgaacgc
      781 cgcgcacacg agcgggtgga tctggatcgc cttgccctcg accagcaccg gctcgaacgc
      841 ctggataccc agacggtgca gcgttggcgc acggttcaag aagaccgggt gctccttgat
      901 gacctcttcg aggacgtccc agacctgcgg gcgggcgcgc tccaccatcc gcttcgcgga
      961 cttgatgttc tgcgcgtact cgaggtcgac gaggcgcttc atgacgaacg gcttgaacag
     1021 ctcgagcgcc atctgcttcg gcagaccgca ctgctgcaag cgcaactgcg gacccacgac
     1081 gatgaccgaa cggcccgagt agtcgacgcg cttgccgagc aggttctgac ggaaccggcc
     1141 ctgcttgccc ttcaacatgt cggagagcga cttgagcgcg cggttgccgg ggcccgtgac
     1201 cggacgcccg cggcggccgt tgtcgaacaa cgcgtcgacg gcctcctgca acatgcgctt
     1261 ctcgttgttg atgatgatct cgggcgcacc gaggtcgagc agtcgcttca gccggttgtt
     1321 gcggttgatc acgcgccggt acaggtcgtt gaggtcggag gtcgcgaagc ggccaccgtc
     1381 gagctgcacc atcgggcgca ggtcgggcgg gatgaccggc acgacgtcga ggatcatgcc
     1441 catcggcgag ttgacgacgc ggccgccgtc gtcgcgccgg ttgaacgcgg acacgacctt
     1501 cagccgcttg atcgccttgg ccttgcgctg acccttggcg gtggcgatgg tctccttgag
     1561 gtcggtctcg acctcgttca ggtcgagacg cgacacgagg tccttcaccg cctcggcacc
     1621 catgccgccc gcgaagtact cgtggtaacg gtcggtgagc tcgcgccaca cgcgctcgtc
     1681 gtcgatcagc tgcttgggct tcagcgactt gagcgtgtcc cagacgagct ggagctgacc
     1741 gatctcctct tcgtagcgga ggcggatctc ttcgaagtcc ttgttcttgg ccttctccgc
     1801 gcgctcggtc tcggccttct tgccgccgcg accctcgatc tcctcgagct ccttgatgta
     1861 ggtctcgtcg cggtcgcgca gcttgacctc acgctcgcgc tcgagctcgt cgatctcggc
     1921 ccgcatctcg gcctcgagcg acgggaggtc ggtgtgcagc ttctcgacgt cgacccacgt
     1981 gatcaggtgg gcggcgaagt agatgacctt ctcgagcgac ttcggcgcga tgtcgagcag
     2041 gtagccgagc cgcgacggga cgcccttgaa gtaccagatg tgcgtgaccg gcgcggcgag
     2101 ctggatgtga cccatgcgct cgcggcgcac cttcgagcgc gtgacctcga cgccgcaacg
     2161 ctcacagatg atgcccttga accggacgcg cttgtacttg ccgcagtagc actcccagtc
     2221 cttcgtcgga ccgaagatct tctcgcagaa gagcccgtcc ttctccggct tgagcgtgcg
     2281 gtagttgatc gtctcgggct tcttcacctc accgttggac caggtgagga tgtcgcccgc
     2341 ggtggcgaga tggatggtga gccggtggct ctcgttcacg tcgaacacgt gattgctcct
     2401 cagtgcctag aagcgtgagc gtgcggtcgc ggcgtcttct tcgtcggtgc cccgttcggg
     2461 tcgtgacaag tcgatgccca gagactcggt ggtgcggaac gcgtcctcgt cgagctcgcg
     2521 gatctcgatc tgctcgccgt tctcgttcat gacgcgaacg ttcaagcaga gcgcctgcat
     2581 ctccttgatc aacaccttga aggactccgg aatgcccggt tccgggatgt tctcgccctt
     2641 cacgatcgcc tcgtacacct tcacgcggcc gagcacgtcg tcggacttga tggtgaggat
     2701 ctcctgcaac gcgtacgcgg atccgtacgc ctcgagcgcc cacacctcca tctctccgaa
     2761 gcgctgacca ccgaactgcg ccttaccgcc gaggggctgc tgcgtgatca tcgagtacgg
     2821 acccgtcgaa cgagcgtgga tcttgtcgtc gacgaggtgc gacagcttca ggatgtacat
     2881 gtagccgacg gtgaccgggc ggtcgtacgc ctggccggtg cggccgtcgt gcaacgtcgc
     2941 cttgccgtct cgaccgatca tgcgctgccc gtctgcgcca tcggggtgca ggtggtcgaa
     3001 cagctcctga atggtcttga cgtggtcgcc ggtctcctcg gtctcgtccc agtgcgcgcc
     3061 gtcgaagacg gggctcgaga tccagaccgc gggctcggtg accgttcgcg tcttgtgggg
     3121 ttcggtgggc ttggcctctt ggccttccca gccgtgccgc gcgacgtagc cgaggtgcga
     3181 ttcgagcacc tggccgacgt tcatgcggct cggcacaccg agcgggttga gcacgatgtc
     3241 gaccggcgtg ccatcggcga ggtacggcat gtcttcgacg ggcaggatct tggcgatcac
     3301 acccttgttg ccgtgacggc cggcgagctt gtcgccctcg ctgatcttgc gcttctggcc
     3361 gacgtacacg cgcaccagct ggttcacgcc cggcggcagc tcgtgaccgt cttcgcgcga
     3421 gaacacgcgg acgtcgatca ccttgccgtt ctcgccgtgc ggcaccttca agctggtgtc
     3481 gcgaacctca cgcgccttct caccgaagat cgcgcgcaac agacgctctt ccggcgtcag
     3541 ctccgtctca cccttcggcg tgaccttgcc gacgaggatg tcgcccgggc cgacctcggc
     3601 gccgatgcgg atgatgccgc gctcgtcgag gtccgtgagg atgtcttcgc tcaggttcgg
     3661 gatgtcgcgc gtgatctctt cggcgccgag cttggtgtcg cgggcgtcga tctcgtgctc
     3721 ctcgatgtgg atcgacgtga gcacgtcgtc cttcaccagg cgctccgaga ggacgatcgc
     3781 gtcctcgaag ttgtggccct tccacggcat gaacgcgacg agcaggttct tgccgagcgc
     3841 gagctcaccg aggtgggtgg aggggccgtc cgcgaggacg tcgcccttct tcacctgcac
     3901 accctccgac accaccggct tttggttgat gcacgtgccc tggttggagc gccggaactt
     3961 ccagaggcgg tgggcggtgc ggcccttctt cgcgtagtcg acggtgatcg tgtcgcccgc
     4021 gacgtcgacg acggtgccgt cgtcttcggc gagcagcacg tcaccggcgt cgcgcgcggc
     4081 gcgggcctcg acaccggtac cgatgtaggg ggcggtcgcc cgcaacagcg gcacggcttg
     4141 gcgctgcatg ttcgcgccca tgagcgcgcg gttggcgtcg tcgtgctcga ggaacgggat
     4201 caacgccgtc gcgacggaca cgagctgctt cggcgagaca tccatgtagt cgacttcgtg
     4261 tgccgtgacg tacgcgatct cgccgcgacc ggctcgcacg agcacgcggt cgtcgaggaa
     4321 gttgccctgc tcgtcgacac gcgcgttcgc ctgggcgatg acgtactcgt cttcgtcgtc
     4381 ggcggcgagg tacgcgatct cgtcggtgac gcggcccttc tcgaccttgc ggtacggcgt
     4441 ctcgatgaac ccgaaccggt tgatgcgtgc gaacgtcgag aggtaaccga tgaggccgat
     4501 gttcgggcct tccggcgtct cgatcgggca catgcggccg tagtggctcg ggtgcacgtc
     4561 tcgcacctcg aacccggcgc gctcacgcga cagaccaccc gggccgagcg ccgagagccg
     4621 gcgcttgtgg gtgagacccg cgagcgggtt cgtctggtcc atgaactgcg acagctgcga
     4681 ggagccgaag aactccttga tcgccgcgac cacgggccgg atgttgatga gcgtctgcgg
     4741 cgtgatcgcc tcgacgtctt gggtggtcat gcgctcgcgc acgacgcgct ccatgcggct
     4801 gaggccgacg cggagctggt tctggatcag ctcgccgacg gtgcgcaccc ggcggttgcc
     4861 gaagtggtcg atgtcgtcgg ggtagtagcc ggcgtcgcgg ttgtgcagct tcacgaggta
     4921 gctgaccgtc gcgaggatgt cggccttggt gagcgtgaag tcgccgctgc ggttctcgac
     4981 gttggcgtcg gggatgtcga ggtcgaagcg ctcgaggccg agcagcgggt actcgcgcga
     5041 cagcttcttc gagaccttgt gccggccgac cttggcctgg tcgtagcgct tggagttgaa
     5101 gaagaggttc tcgagcagcg cgcgcgccga gtcgggcgtc ggcggctcgc cgggacgcag
     5161 cttgcggtag atgtcgatga acgcctcgtc gacggtggtc gtgttgtcct tgtcgagcgt
     5221 gttgcgcatc gactcgtacg ggcgaccgac ttcgtcggtg atgaggttga gcagctcgtc
     5281 gtcgctctca ccgaagccga gcgccttgag caggatggtg accggctgct tgcgcttgcg
     5341 gtcgatgcgg acgtaggcga tgtccttctt gtcgacctcg aactcgagcc acgcgccgcg
     5401 acccgggatc atcttggcgt cgacgacgtc cttctcgg
//
LOCUS       DASUGA010000836         6773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_520034, whole genome shotgun sequence.
ACCESSION   DASUGA010000836 DASUGA010000000
VERSION     DASUGA010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6773
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520034"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..1071)
                     /locus_tag="VFX21_16510"
     CDS             complement(181..1071)
                     /locus_tag="VFX21_16510"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5097628.1"
                     /translation="MRRCFAVLTSIAAFAVISPTSSSTASAADVRPGFTTWTETFVDT
                     SRPTVPNTGPAVPSRTLVTTIYKPNGHGPFPAVMFSHGYVGYPEKFTKLFAAWASAGF
                     VVVAPTFPLTNGHLASPKAADYVNQPGDVSFVLDRVLALDKQRGSRLSHAIDDHKIGA
                     GGLSLGGLTTYILGYDACCRDARIDAVMVLDGIRSSGVLDGHVPLLIAHSDADPVIPY
                     STARAAFDDAAAPAWLVTFFGASHASQWEDTVTPYDAIAERVTTDFWNATLNHKRRSL
                     TTLEHDATVPGLSMIEAKGR"
     gene            complement(1115..2152)
                     /locus_tag="VFX21_16515"
     CDS             complement(1115..2152)
                     /locus_tag="VFX21_16515"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5097629.1"
                     /translation="MRRERPLILAVDDEPASLALLHKILESEGYDVVEATDGASTLQA
                     IAEHKPDLVCLDVMMPAPGGIEVCQRVRRDPEHAGLPILLLTALDRIEDKVRGLEAGA
                     DDFLSKPFDLSELTARVRSALRMKALQDRLADLLGRYVSGNVATEVLRDPYAVSLGGD
                     RRDVSTLFVDVRGYTALAAEHPPETILEVLNRYLAVVTDAVERHDGTVAGFLGDGVIA
                     FFGAPVKHADDPERAVRSALAMQREVMSLEIPTLRGVRLHLGIGITSGEVIAGNVGSE
                     RRMHYTVIGDPVNVASRLQTAAGPGQILVDEPTHDKVRDLVASQDLGSLRLAGKGDWV
                     RAFNVLALQST"
     gene            complement(2149..2514)
                     /locus_tag="VFX21_16520"
     CDS             complement(2149..2514)
                     /locus_tag="VFX21_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5097630.1"
                     /translation="MATILVIEDTAANRALATKLLQAAGHDVLSAETATDGMALAREQ
                     LPDLVLLDLGLPDMDGWQALRELRADHGPVAKLRIVAFTAHAMVGDDERALAAGFDEY
                     LSKPIDYATFADTIQSLLP"
     gene            complement(2514..4520)
                     /locus_tag="VFX21_16525"
     CDS             complement(2514..4520)
                     /locus_tag="VFX21_16525"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5097631.1"
                     /translation="MARDQVMTVAEPTLPRFTGWLRPVVDAVARIKAGVHRKLLFGFF
                     IGALLLIAMALLSLLVIRQMNDRRVELNDAQVKAGRAQEMLYAVTAQSHYRAMALLLP
                     DDAVKYNGQVEDAKATFAGLLEDLANAEPDNAAFYDGVRSVNEDYRESGQQVLALINA
                     DRTDEARDIHLNEEHPTSHKLEASMRTLIGEAKQDMADAQSDFRSAHDLFTKMVIAFS
                     AASVIVALLLGFVLSWAFVLPVRKMEIALADITAGNFDARVEVPNRDEFGGLARDLNS
                     TSERLATLFDDQRLLAARLSETNVSLERSSEAKSRFLANVSHELRTPMTAILGFTDAL
                     LAGVDGPLNSEQETSLRWVQRGGQDLLGLINEILDLSKIEAGKLTLDVERFDPRELVD
                     VVVAQHRSLAAQKGIGFGWRDAGTPPEVALDRQRVRQILVNLVGNALKFTDAGEVEVV
                     AGGSDDARFNVAVRDTGPGIAPEMQEMIFEEFHHAEGAAAGSGLGLPISRRLARAMGG
                     DVTFESEPGRGSVFFLVLPLSCRPTPADSDELPPVAASDGERVLLCVDDDPSVCHVLQ
                     KMVSGHGYRVVEAPSATAFEDARREQPAAILIDVRLQERDALDIVRELKRNPETNAIR
                     VIVMSGSDLTELPEGVDRSLRKPVQQPRLLRVLTDDATAQDAWL"
     gene            complement(4640..5599)
                     /locus_tag="VFX21_16530"
     CDS             complement(4640..5599)
                     /locus_tag="VFX21_16530"
                     /inference="COORDINATES: protein motif:HMM:NF039286.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5097632.1"
                     /translation="MGRRFPFPIPFGWFQVAFPSDLAPGEVTALTYWNRELVLWRDDA
                     GDYHLQDAFCPHLGAPLGVGGKVKGDQLECPFHGWTYDGAGACTNIPYSQRSNKKAKL
                     RTYPTIMRNGFVLAWYHPFEAEPSWEIPVIEEIGDSGWTDFYSTSYIVNTVPQEMSEN
                     GADPAHFRYVHGTDEVAEMISYDTEGPCSVMLSKQAFVTPRGVTHGRIDVYNYGPGFS
                     KVWFSGIVDAINIATTTPIDEERTQVRFNFVVRKFDDAKLTSTVADAFVKEINKQVQE
                     DRPIWEHKTFLPTPALADTDGPIMKFRKWYSQFYAEPYEVPVG"
     gene            complement(5743..>6773)
                     /locus_tag="VFX21_16535"
     CDS             complement(5743..>6773)
                     /locus_tag="VFX21_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HEX5097633.1"
                     /translation="DIDAVVIGKAPDMFEGVGMPELYLADALGATGKPMMRVHTAGSV
                     GGSTSIVAAKLVAAGIHERVLTVAFEKQSESNATWGLSVAMPFMAPLVAGAGGYFAPL
                     IRAYMTRSGAPRHIGHLVAVKDRLAAVRNPKAHLHLPDITLQEVEESFLLWDPLRYLD
                     VCPSSDGAMALVVGSEKVADAAPNPPAWIHGMAMRSEPTMFARRDQVSPQAGRDCAKD
                     VYAQAGINNPRDDFDCAEVYVPFSWYEPMWLENLGFCAEGEGWKVTESGATAPDGDIP
                     WNPSGGVLSSNPIGASGMVRFGEAAQQVRGMAGDYQVELKTGRALGQAYGGGSQFFSM
                     WVVGRDKP"
BASE COUNT         1077 a         2284 c         2257 g         1155 t
ORIGIN      
        1 caatgggagt acatcctgcc gacgagcctt accgttgccg acgcggccgc gccgacgggg
       61 acgctgcagg cgttgttcgc cgcgatcgtg ctcttcgtgc tcgtcgtcgt gccgggcttc
      121 gtgctgctct acacactcga ccagaagagt ctgctgccgg gcgaaggcgt cgactgaggg
      181 ctaacggcct ttcgcctcga tcatcgacag cccgggaacg gtcgcgtcgt gttcgagcgt
      241 cgtcaggctc cttcgcttgt ggttgagcgt cgcgttccag aagtcggtcg tcactcgttc
      301 ggcaatggcg tcgtagggcg tcaccgtgtc ctcccattgc gacgcgtgcg acgcgccgaa
      361 gaacgtcacg agccatgccg gcgcggccgc gtcgtcgaat gcggctcggg ccgtgctgta
      421 cgggatcacc gggtcggcgt ctgagtgtgc gatgagcaac ggcacatgac cgtcgagcac
      481 accactgctg cgaattccgt ccagcaccat caccgcgtcg atgcgcgcgt cgcgacagca
      541 ggcgtcgtaa cccaggatgt aggtcgtcaa gccgccgagt gacaggccgc cggcgccgat
      601 cttgtggtcg tcgatcgcat gggagaggcg gcttcctcgc tgcttgtcga gcgcgaggac
      661 tcggtcgagc acgaacgaca catcgcccgg ctgattgacg taatcggcag ccttcgggct
      721 cgcgagatga ccgttcgtga gcgggaacgt cggtgccacc accacgaacc cggcgctcgc
      781 ccacgccgcg aacagcttcg tgaacttctc cgggtagccg acgtagccgt gcgagaacat
      841 gaccgccggg aacgggccgt gcccgttcgg tttgtagatc gtcgtgacga gcgtccgtga
      901 cggcaccgcc ggaccggtgt tcggcaccgt cggacgcgac gtgtcgacga aggtctccgt
      961 ccacgtcgtg aaccccgggc gaacatcagc cgccgacgcc gtcgatgacg acgtcggcga
     1021 aatcaccgcg aacgccgcga tcgacgtcag cactgcgaag catcgtctca cgagcggcga
     1081 gtctggtgca gcgccgcacg atgcgccaag cgccctacgt ggattgcagc gcgagcacgt
     1141 tgaaggcgcg cacccagtcg cctttgccgg cgaggcgcag gctgccgagg tcttgcgacg
     1201 cgacgagatc tcgcactttg tcgtgcgtcg gctcgtcgac gaggatctga ccgggaccgg
     1261 cggcagtctg cagtcgcgac gcgacgttca ccgggtcacc gatgacggtg tagtgcatcc
     1321 ggcgctccga cccgacgttg ccggcaatca cctcgcccga cgtgatgccg atgccgaggt
     1381 gcagtcgcac gccgcgcagc gtcgggatct cgagcgacat gacctcgcgc tgcatggcga
     1441 gggcggatcg caccgctcgc tccggatcgt cggcgtgttt caccggcgca ccgaagaacg
     1501 cgatgacgcc gtcgcccagg aagcccgcaa cggtgccgtc gtgacgttcg accgcatcgg
     1561 tgacgaccgc gaggtaccgg ttcagcacct ccaagatcgt ttccggtgga tgttccgcgg
     1621 cgagtgccgt atagccgcgc acgtcgacga acagcgtcga cacgtcgcgt cgatcgccgc
     1681 cgagcgagac ggcgtacgga tcgcgcagca cctccgtcgc gacgttcccg ctgacgtatc
     1741 ggcccagcag gtcggcgagc cggtcctgca gcgccttcat gcgcagcgcg gagcgcactc
     1801 gcgcggtcag ctcgctcaag tcgaatggtt tgctcaggaa gtcgtcggcc cccgcctcca
     1861 ggccgcgcac cttgtcctcg atgcggtcga gggccgtgag cagcaggatg ggcaagccgg
     1921 catgctcggg atcgcgacgc acgcgctggc agacttcgat acctccgggc gcgggcatca
     1981 tcacgtcgag gcagacgagg tcgggcttgt gctcggcgat cgcctgcagc gtcgatgcgc
     2041 cgtccgtcgc ttcgacgacg tcgtagccct cactctccag gatcttgtgc agcagcgcca
     2101 agctcgcggg ctcgtcgtcc acggcaagga tgagtggccg ctcgcgcctc acggcagcag
     2161 gctctgaatc gtgtcggcga aggtcgcgta gtcgatcggc ttgctcaagt attcgtcgaa
     2221 gccggccgcc agcgcgcgtt cgtcgtcgcc gaccatcgcg tgcgcagtga acgcgacaat
     2281 acgcagcttc gcgaccggac cgtggtcggc acggagctca cggagcgctt gccagccgtc
     2341 catgtcaggg agcccgagat ccaggagcac gaggtcgggg agctgctcgc gggcgagggc
     2401 catgccgtcg gtcgccgtct cggccgagag cacgtcgtgg cccgcggctt gcagcagctt
     2461 ggtggccagc gcccggttcg ccgccgtgtc ctcgatcaca aggatcgtcg ccattagagc
     2521 caagcatcct gtgccgtggc gtcgtcggtg agcacgcgca ggagccgggg ctgctggacc
     2581 ggcttgcgga gcgaacgatc gacgccctcc ggcagttcgg tcaggtcgga acccgacatc
     2641 acgatcactc ggatcgcgtt ggtctcgggg ttgcgcttga gctcgcgcac aatgtccagc
     2701 gcgtcgcgtt cctggaggcg aacgtcgatc aggattgcag ccggctgctc acgccgcgcg
     2761 tcctcgaagg cagtcgcgct cggtgcttcg acgacgcggt acccatggcc gctgaccatc
     2821 ttttgcagca cgtgacacac cgatgggtca tcatcgacgc acaacaacac gcgctcacca
     2881 tcggacgcgg caaccggagg aagttcgtcg ctgtcagccg gtgtcggacg acaactgagc
     2941 gggagaacaa ggaagaagac actgccgcgg cccggctcgc tctcgaatgt gacgtcgccg
     3001 cccatcgcgc gtgcaagacg acggctgatc ggcagcccga gaccgctgcc cgcggcggcc
     3061 ccctcggcgt gatggaactc ttcgaagatc atctcctgca tctccggggc gataccgggc
     3121 ccggtgtcgc gcaccgcgac gttgaaacga gcgtcgtcgc ttcctccggc gacaacctcc
     3181 acttcgcccg catcggtgaa cttcagcgcg ttcccgacca ggttgacgag gatctggcgc
     3241 acacgctggc ggtcgagcgc gacttcgggc ggcgtccccg cgtcgcgcca tccgaagccg
     3301 atccccttct gcgccgcgag tgaacgatgc tgtgccacga cgacgtcgac gagctctcgc
     3361 gggtcgaagc gctcgacgtc gagggtcagc ttcccggcct cgatcttcga caggtccagg
     3421 atctcgttga tcaatccgag caagtcctgc ccgccccgct ggacccagcg cagcgacgtc
     3481 tcctgctccg agttgagcgg accgtccaca cccgcgagga gcgcatcggt gaagccgagg
     3541 atggcggtca tcggcgtgcg cagctcgtga ctgacgttcg ccaggaatcg tgacttcgcc
     3601 tcgctgctcc gctcgagcga cacattcgtc tcgctcagtc gcgcggcgag gagtcgctga
     3661 tcgtcgaaca gcgtggccag acgttcgctc gtgctgttga gatctcgcgc gagcccaccg
     3721 aactcgtcgc ggttcggtac ctcgacgcgc gcgtcgaagt tgccggcggt gatgtcggcg
     3781 agtgcgatct ccatcttccg gaccggcagg acgaacgccc atgagaggac gaacccgagc
     3841 agcagggcga cgatgacgct tgcggccgag aacgcgatca ccatcttggt gaagaggtcg
     3901 tgcgcagacc ggaagtcgct ctgcgcgtcg gccatgtcct gcttggcttc tccgatcagc
     3961 gttctcatcg acgcttcgag cttgtgcgac gtcgggtgct cctcattcag gtggatgtca
     4021 cgagcttcgt cggtacggtc tgcgttgatc agcgccagca cctgttggcc cgattcgcga
     4081 tagtcctcgt tcacggaccg gacgccgtcg tagaacgccg cgttgtcggg ctcggcgttc
     4141 gccaagtctt cgaggaggcc ggcgaaggtc gccttggcgt cctcgacttg cccgttgtac
     4201 ttcacggcgt cgtccgggag gagcagtgcc atcgcacggt agtgactctg ggccgtcacc
     4261 gcgtagagca tctcctgcgc gcgaccggcc ttcacttgcg cgtcgttcag ctcgacccgg
     4321 cgatcgttca tctggcggat cacgaggagg ctgagcagcg ccatcgcgat gagcaacaag
     4381 gcgccgatga agaagccgaa gagcaacttg cggtggacgc cggctttgat gcgggcgacc
     4441 gcgtcgacga ccggacgcag ccatccggtg aagcgcggca acgtcggttc ggcgaccgtc
     4501 atcacctggt cacgagccat caccggctcc gatctcagcg tgcggcgctc ggccgcattc
     4561 ggcacatccc cgctctcgac gggtcagtct cgccgagttc gggagcctgc gtaaaccgcc
     4621 gcgcgctcga gcccgtcgct cagccgacgg ggacctcgta cggttccgcg tagaactggc
     4681 tgtaccactt gcggaacttc atgatcggac cgtcggtgtc ggcgagggcc ggcgtcggca
     4741 ggaacgtctt gtgctcccag atcgggcggt cttcctgcac ctgcttgttg atctccttga
     4801 cgaacgcgtc ggcgacggtc gacgtcagct tcgcgtcgtc gaacttgcgg acgacgaagt
     4861 tgaaccgcac ctgcgtgcgc tcttcgtcga tcggggtcgt cgtggcgatg ttgatcgcgt
     4921 cgacgatgcc gctgaaccac accttcgaga agccgggccc gtagttgtag acgtcgatgc
     4981 ggccgtgtgt gacaccgcgc ggcgtgacga acgcttgctt cgacagcatc accgagcacg
     5041 gcccttcggt gtcgtacgag atcatctccg cgacttcgtc ggtaccgtgc acgtaacgga
     5101 agtgcgcagg gtcggcgccg ttctccgaca tctcctgcgg caccgtgttg acgatgtacg
     5161 acgtcgagta gaagtcggtc cagccgctgt cgccgatctc ctcgatgacc gggatctccc
     5221 acgacggctc ggcttcgaac gggtggtacc acgcgagcac gaaaccgttc cgcatgatcg
     5281 tcggatacgt gcgcagcttc gccttcttgt tcgaccgctg gctgtacggg atgttcgtgc
     5341 acgcgccggc gccgtcgtag gtccagccgt ggaacgggca ctcgagctgg tcgcccttca
     5401 ccttgccgcc gacaccgagg ggcgcgccga ggtgcgggca gaacgcgtct tgcaggtggt
     5461 agtcgccggc atcgtcgcgc cacagcacga gctcgcggtt ccagtacgtc agcgccgtca
     5521 cctcgcccgg cgcgaggtcg ctcgggaacg cgacctggaa ccagccgaac gggatcggga
     5581 acgggaagcg acgacccata ggcctcgatt ttagaacagg ttctattcct gtgccagcag
     5641 cctcaggcgt gggcccggcg cagggcatcg atcgcgtccg cgcctcgtac cggcgccgct
     5701 cacccaccgt cgcggtggtt caggggcgca ggtacgcgcg ggtcacggct tgtcgcgacc
     5761 gaccacccac atcgagaaga actgcgaacc gccgccgtag gcctggccga gcgcccggcc
     5821 ggtcttcaac tcgacctggt agtcgccggc catgccgcgc acttgttgcg cggcttcacc
     5881 gaagcggacc atgccggacg cgccgatcgg gttcgacgac aacacgccgc ccgacgggtt
     5941 ccacggaatg tcgccgtcgg gtgcggtcgc gccggactcg gtgaccttcc agccttcgcc
     6001 ctccgcgcag aacccgaggt tctcgagcca catcggctcg taccacgaga acggcacgta
     6061 cacctcggcg cagtcgaagt cgtcgcgcgg gttgttgatc ccggcctgcg cgtacacgtc
     6121 cttggcgcag tcgcgtcctg cctgcgggct gacctggtcg cggcgggcga acatcgtcgg
     6181 ctcgctgcgc atcgccatcc cgtggatcca cgcgggcggg ttgggcgcgg cgtccgcgac
     6241 cttctcgctg ccgacgacga gcgccatcgc gccgtcggag gacgggcaga cgtcgaggta
     6301 gcgcagcggg tcccacaaca ggaacgactc ctcgacctct tgcagcgtga tgtcgggcag
     6361 gtgcaagtgc gccttcgggt tgcgcaccgc cgcgagccgg tccttcaccg cgacgagatg
     6421 gccgatgtgg cgcggcgcgc ccgagcgcgt catgtacgcg cggatgagcg gcgcgaagta
     6481 gccacccgca ccggcgacga gcggcgccat gaacggcatc gcgacggaga gaccccacgt
     6541 cgcgttgctc tccgactgct tctcgaacgc gaccgtcagg acccgttcgt ggatcccggc
     6601 cgcgacgagc ttggccgcaa cgatcgacgt cgagccgccg accgaacccg cggtgtgcac
     6661 gcgcatcatc ggcttgcccg tcgcgccgag cgcgtcggcg aggtacagct cgggcatgcc
     6721 gacgccctcg aacatgtctg gtgccttgcc gatcacgact gcgtcgatgt cgg
//
LOCUS       DASUGA010000837         5084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_242518, whole genome shotgun sequence.
ACCESSION   DASUGA010000837 DASUGA010000000
VERSION     DASUGA010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242518"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="VFX21_16540"
     CDS             <1..1215
                     /locus_tag="VFX21_16540"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5097634.1"
                     /translation="IRAMDFEFSPEQQAFAAEVEQFLDENDDPEVFDLTRENMAQIVD
                     TPKRRAFMRSLGERGWLGLTWPKAYGGSEGAGVYEFLLNEQLAGRGGPQIGKGVGIIG
                     KTLIAHGSDFLKEKFLPMILNNDVEFAVGYSEPNAGSDAASMRLKAERGERDGKQGWI
                     LNGQKTWTTSAHFAEWYWLGTRTDPNNKHKGITLMLVPLDQPGITINGIWTMGDERTN
                     EVFIEDVFVPDEYVVGDVGSGFQYISQALDLERFTMFTFSPIKQRLDLLCDFVRNETR
                     DGKALKDDPVIRRDIAQLVTQAEVARVLGLRVVAASMKGEDTPGAPPPTTEASEYKLY
                     ATEFSRRLANASMDIGGPSTQLRVKTKEAPMEGRAESTYRYTVIDTIGGGTSEVQKNI
                     IARRALGLPKNF"
     gene            1222..2376
                     /locus_tag="VFX21_16545"
     CDS             1222..2376
                     /locus_tag="VFX21_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015793945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX5097635.1"
                     /translation="MLDGITVLDLASVGPAARTSRWLADYGARVVKVGPPPKQSGVQI
                     VPPFYAYSGHRDMQRVLLDLKAPAGRDAFLRLAAEADVLIESFRPGVMARLGLGDDDV
                     RAVNPRIVYCSTSGYGQSGPRSQWAGHDLNYLAVGGYLDCTGRDANGGPALPGATIAD
                     SAGGGMQAVIAILAALVQRASTGVGAYLDVSVADGVVALMSLYVDEFLATGEVPGPRH
                     NILTGRYACYDVYRCFDDKWVAVAAIEPHFYANLCKALACEQWLAHQQDDAVQDDIRQ
                     DFAAAFARRPRDEWVATLGPGDTCVSEVATVPELVRDEHYRARHVFADAHAPPNGAFE
                     QVNRVLAGQIRADTTIELREPTRTDTDELLAAVGFTESEIAAMREEGVAA"
     gene            2373..2876
                     /locus_tag="VFX21_16550"
     CDS             2373..2876
                     /locus_tag="VFX21_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5097636.1"
                     /translation="MNKTAADRIGEIQYEEAGEFPVERGYIWTSCASVENGNPLFWDD
                     DVAAEITDGPIAPPTMLSVWFRPHHWAPGRTEQALPLQVHFDLKAHFGLPEAVMTDNT
                     IVFHEPVRPDDRLRTRQILRSISEPKTTKLGTGRFWVIDVEYLNQRDELVGVESYTGF
                     GYKREAS"
     gene            2873..3337
                     /locus_tag="VFX21_16555"
     CDS             2873..3337
                     /locus_tag="VFX21_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097637.1"
                     /translation="MNLDDVAKGDVLPELRYDVTATTVVLGALAARDWRPMHHDHDFA
                     VNRNGTQDIFLNTPNQAAWFERYLTDWSGPKGRLGRMKFRMKGSVFPGDTMVLSGTVE
                     DVTTDDAGCGWVNVLVTLTVDGDLKTDCTARIALPTSDNDNPWRRRGDQWKP"
     gene            3346..4455
                     /locus_tag="VFX21_16560"
     CDS             3346..4455
                     /locus_tag="VFX21_16560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5097638.1"
                     /translation="MDLDLSEEQLMLRDMVRGICTSYAPLETVRALEDDPTGYSAELW
                     KQMAELDLIGLMLPARYGGSEMTLLEGAVVYAEFGRALASTPHFVSAVLAAGVLLRAG
                     SDAQKDALLPNIVSGKDIVSVGWLEPHGAYSPAGVQLRATADGDGFRLDGVKWHVPFA
                     SSASHFVVLGRAGDRVDLFLVDAKASGLTPAQHKTIASDAQYEIAFDGVAAQRIGGAG
                     TGWATFEATLLDGIILLAAQAIGGARYALEITTQYAKDREQFDKPLGAFQAIAHYLAD
                     AATTVDGGEVLVYEAAWAAANGRDIARLAPMAKLFACKTFRDVTATAQQIFGGVGFTV
                     EYDIQLYFRRAKQQQLSWLDDRALEELIATDVLDR"
     gene            4460..4945
                     /locus_tag="VFX21_16565"
     CDS             4460..4945
                     /locus_tag="VFX21_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5097639.1"
                     /translation="MRDFPVTGRSGMYKSIVVGTDGSDTAAFAVNHAIELAKATGATL
                     HVVTAHHKLSPGIAALGTEAGGPVFDTHEVNQSIALQSEKICEVALRSAKDAGVPAES
                     HVQSGDPADVLVAVAEAVDADLVVVGNRGMSGVKRFVLGSVPNKVSHHCPCHLLIVQT
                     C"
BASE COUNT          893 a         1734 c         1703 g          754 t
ORIGIN      
        1 atccgggcca tggacttcga attctcgccg gagcaacagg cgtttgcggc cgaggtcgag
       61 cagttcctcg acgagaacga cgatcccgag gtcttcgacc tcacgcgcga gaacatggcg
      121 cagatcgtcg acacgcccaa gcgccgcgcc ttcatgcggt cgttgggtga gcgcgggtgg
      181 ctcgggctca cgtggccgaa ggcctacggc ggttccgagg gcgcaggcgt ctacgagttc
      241 ctgctgaacg aacagctggc cggccgcggc ggtccgcaga tcggcaaggg tgtcggcatc
      301 atcggcaaga cactcatcgc gcacggcagc gacttcctca aagagaagtt cctgccgatg
      361 atcctcaaca acgacgtcga gttcgcggtc ggttacagcg aaccgaacgc cggctccgac
      421 gccgcgtcga tgcgcctgaa ggccgaacgc ggcgaacgcg acggcaagca gggctggatc
      481 ctcaacgggc agaagacgtg gacgacatcc gcgcacttcg ccgagtggta ctggctcggc
      541 acccgcaccg acccgaacaa caagcacaag ggcatcacgc tgatgctcgt gcccctcgac
      601 cagcccggta tcacgatcaa cggcatctgg acgatgggcg acgagcgcac caacgaagtc
      661 ttcatcgaag acgtcttcgt gcccgacgaa tacgtcgtcg gtgacgtcgg cagcggcttc
      721 cagtacatct cgcaggcgct cgacctcgag cgcttcacca tgttcacgtt ctcgccgatc
      781 aagcagcgcc tcgacctctt gtgcgacttc gtgcgcaacg agacgcgcga cggcaaggcg
      841 ctgaaggacg accccgtcat ccgccgcgac atcgcgcagc tcgtcacgca ggccgaagtc
      901 gcgcgagtgc tcggcctgcg cgtcgtcgcg gcgtcgatga agggggaaga cacgccgggc
      961 gcgccgccac cgaccaccga ggcatccgag tacaagctgt acgcgacgga gttctcgcgc
     1021 cggctggcga acgcgtcgat ggacatcggc ggcccgagca cgcaactgcg cgtgaagacc
     1081 aaggaagcgc ccatggaagg ccgcgccgag tcgacgtacc gctacaccgt catcgacacg
     1141 atcggtggcg gcacgtccga ggtgcagaag aacatcatcg cccggcgcgc gctcggcctc
     1201 ccgaagaact tctgaccgac cgtgctcgac ggcatcaccg tgctcgacct cgcgtcggtg
     1261 ggtcccgcgg cgcgtacgtc gcgctggctc gcggactacg gcgcgcgcgt cgtgaaggtc
     1321 gggccgcccc cgaagcagtc gggcgtgcag atcgtgccgc ctttctacgc gtacagcggg
     1381 caccgcgaca tgcagcgcgt cctgctcgac ttgaaggcgc cggccgggcg cgacgcgttt
     1441 ctgcggcttg cggccgaagc cgacgtgctg atcgagagct tccggcccgg cgtgatggcg
     1501 cgcctcgggc tcggtgacga cgacgtgcgc gcggtgaacc cgcgcatcgt ctactgctcc
     1561 acgagcgggt acgggcaatc gggcccgcgt tcgcagtggg ccggtcacga cctcaactac
     1621 ctcgcggtcg gcggctacct cgactgcacg ggtcgggacg caaacggcgg gccggcactg
     1681 ccgggcgcga cgatcgccga cagcgccggc ggtggcatgc aagcggtgat cgcgatcctc
     1741 gccgcactcg tgcagcgcgc atcgaccggc gtcggcgcct atctcgacgt ctcggtcgcc
     1801 gacggcgtcg tcgcgctgat gtcgctgtac gtcgacgagt tcctcgcgac cggcgaggtg
     1861 cccgggccgc gtcacaacat cctgaccggc cgctacgcgt gctacgacgt gtaccgctgc
     1921 ttcgacgaca agtgggtcgc ggtcgccgcg atcgagcccc acttctacgc gaacttgtgc
     1981 aaggcgctcg cctgcgagca gtggctcgcg caccaacagg acgacgcagt gcaagacgac
     2041 atccgccaag acttcgcggc cgcgttcgcg cggcgtccac gcgacgagtg ggtcgcaacg
     2101 ctcgggccgg gcgacacctg cgtgtcggag gtggcgacgg tgccggagtt ggtgcgcgac
     2161 gagcactacc gggcccgcca tgtcttcgcc gacgcccacg cgccgccgaa cggcgcattc
     2221 gagcaagtga accgcgtgct cgcgggccag atccgcgcgg acacgacgat cgaactgcgc
     2281 gaaccgacgc gcaccgacac cgacgagctg ctcgcagccg tcggcttcac cgaatcggag
     2341 atcgcggcca tgcgcgagga aggagtcgcc gcgtgaacaa aaccgccgcc gatcggatcg
     2401 gcgagatcca gtacgaggag gcgggcgaat tcccggtcga gcgcggctac atctggacgt
     2461 cgtgcgcgtc ggtcgagaac ggcaacccgt tgttctggga cgacgacgtc gcggccgaga
     2521 tcaccgacgg tccgatcgcg ccgccgacga tgctgtcggt gtggttccgg ccgcaccact
     2581 gggcgccggg ccgcaccgag caggcgctcc cgctacaggt gcacttcgac ttgaaggcac
     2641 acttcgggct gcccgaagcg gtgatgaccg acaacacgat cgtcttccac gaacccgttc
     2701 gccccgacga caggttgcgc acccgccaga tcctgcgttc gatcagcgag ccgaagacga
     2761 cgaagctcgg caccggccgc ttctgggtca tcgacgtcga gtacctgaac cagcgcgacg
     2821 agctggtcgg cgtcgagtcg tacacgggct tcggctacaa acgggaggcg tcgtgaacct
     2881 cgacgacgtt gccaagggtg atgtgcttcc ggagctgcgt tacgacgtca ccgcgacgac
     2941 ggtcgtgctc ggcgcactcg ccgcgcgcga ctggcgcccg atgcaccacg accacgactt
     3001 cgcagtcaac cgcaacggca cgcaagacat cttcctgaac acgccgaacc aagcagcatg
     3061 gttcgagcgc tatctcaccg actggtcggg cccgaagggc cgcctcgggc ggatgaagtt
     3121 caggatgaag ggctcggtgt tccccggcga cacgatggtg ttgtccggca cggtcgaaga
     3181 cgtcacgacc gacgacgccg ggtgcggatg ggtcaacgtg ctcgtcacgc tgaccgtcga
     3241 cggcgacctc aagaccgatt gcacggcgcg catcgcgctg ccgacatccg acaacgacaa
     3301 tccctggcgc cgccgcggcg accagtggaa gccctagggg aacacgtgga cctcgatctc
     3361 tccgaagaac agctcatgct gcgcgacatg gtgcgcggca tctgcacgtc ctacgcgccg
     3421 ctggagacgg tgcgcgcact cgaggacgac cccaccgggt actcggccga gctgtggaag
     3481 cagatggccg agctcgacct catcgggctg atgctgcccg cgcgctacgg cgggtcggag
     3541 atgacgctgc tcgaaggcgc ggtcgtgtac gccgagttcg gtcgcgccct ggcctccacg
     3601 ccgcacttcg tcagcgcggt gctcgcagcg ggcgtgctgc tgcgcgcggg ctcggacgcg
     3661 cagaaggacg cgctcctgcc gaacatcgtg agcggcaagg acatcgtctc ggtcggctgg
     3721 ctcgaaccgc acggcgcgta ctcaccggcc ggtgtgcagc tgcgggcaac ggccgacggt
     3781 gacgggttcc ggctcgacgg cgtgaagtgg cacgtgccgt tcgcgtcatc ggcgtcgcac
     3841 ttcgtcgtgc tcggccgcgc cggcgaccgc gtcgacctgt tcctcgtcga tgcgaaggca
     3901 tcgggcctca cgccggcgca gcacaagacg atcgcgtcgg acgcgcagta cgagatcgcg
     3961 ttcgacggcg tcgccgccca acgcatcggt ggcgcgggga cgggctgggc gacgttcgag
     4021 gcaacgttgc tcgacggcat catcctcctc gcggcgcagg cgatcggcgg tgcccgctac
     4081 gcgctcgaga tcacgacgca gtacgccaag gaccgggagc agttcgacaa gccactcggc
     4141 gcgttccagg cgatcgcgca ctacctcgcc gacgcggcaa ccaccgtcga cggcggcgag
     4201 gtactggtct acgaggctgc ctgggccgcc gccaacggcc gcgacattgc ccggctcgcg
     4261 ccgatggcga agctgttcgc atgcaagacg ttccgcgatg tcaccgcgac cgcgcagcag
     4321 atcttcggcg gcgtcgggtt caccgtcgaa tatgacatcc agctgtactt ccgtcgcgcc
     4381 aagcagcagc aactctcgtg gctcgatgac cgcgcgctgg aagagctcat cgccaccgac
     4441 gtgctcgacc gttgacatcg tgcgagactt cccggtgacg gggaggtcag ggatgtacaa
     4501 gtcgatcgtc gtcggcacgg acggctcgga cactgcagcg ttcgcggtca atcacgcgat
     4561 cgagctcgcg aaggcgaccg gcgcgacact gcacgtcgtc accgcccacc acaagttgtc
     4621 gccgggcatc gccgcgctcg gtaccgaagc gggcggaccg gtcttcgaca cgcacgaggt
     4681 gaaccagtcg atcgcgctcc agagcgagaa gatctgtgaa gtcgccctgc gcagtgccaa
     4741 ggacgcgggc gttccggccg agagtcacgt ccagtccggc gacccggccg acgttctcgt
     4801 cgccgtcgcg gaagccgtcg acgcggacct ggtcgtcgtc ggcaaccgcg gcatgagcgg
     4861 cgtcaagcgc ttcgtgctcg gcagcgtccc gaacaaggtg tcgcaccact gcccctgcca
     4921 tctgctgatc gtgcagacct gttaggcgaa gaactcgtgc gcctcaatgt cgggcgatag
     4981 aggcaagacc actcgaagct aggcacccgt ccagtggatg tctcgaagcc agaaatcacc
     5041 gactccgttg tcgtcgatca gctcggaagt atgaagctct ttga
//
LOCUS       DASUGA010000838         2100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1632597, whole genome shotgun sequence.
ACCESSION   DASUGA010000838 DASUGA010000000
VERSION     DASUGA010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632597"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1109
                     /locus_tag="VFX21_16570"
     CDS             141..1109
                     /locus_tag="VFX21_16570"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEX5097640.1"
                     /translation="MELVTKKKLTFVAGRGHEELSSEVASALQVPVGDVVHSTFANGE
                     IYCRYGENIRGADVFVFQSHCDPINDRIMEQLLMIDAAKRASAKRITAVIPYYGYARQ
                     DRKAEGREPISARLLADMLTAAGAQRVVSVDLHTGQIQGFFDFPVDHLTAVPVLAAHL
                     ATEIDSESVIVAPDAGGGKLARRYADRLGADIAFIDKRRPKGTHNVAVATEVVGDIDG
                     RQCVIVDDIIDTAGTVVSAANLLIERGASEVYIAATHGLLSGPAVDRLKNGPIREVVV
                     TNTVPVPSEKRFDGLRVLSIAPLLARAIDAVFDDRSVSELFGGDNV"
     gene            1197..1853
                     /locus_tag="VFX21_16575"
     CDS             1197..1853
                     /locus_tag="VFX21_16575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX5097641.1"
                     /translation="MSEATLTADPRTETGSRAAGRYRREGLVPAVVYGLETEATSVTV
                     NARELQRILSGASGANTLITLKVDGGDKLALARQIHRHPVKGTLVHVDFVLVRADQEI
                     AADVPIHLEGEAEGAKRGGLLEQLMFSIGITALPRDIPQSIAHDVSGMEIGDQLHVSD
                     LVIPAGVTVTNDLDELVVQITQPRGMEEEEAAEGEAAEGEAPAEGAAPAAAESSDSGE
                     "
     gene            1857..>2100
                     /locus_tag="VFX21_16580"
     CDS             1857..>2100
                     /locus_tag="VFX21_16580"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX5097642.1"
                     /translation="MVADLLVVGLGNPGAEYARTRHNLGAEVVEILAKRHGARLRKTK
                     DRALVDDIRLGGKQIVLAVPTTYMNDSGVAIGAMVKR"
BASE COUNT          356 a          718 c          729 g          297 t
ORIGIN      
        1 ccggtcgagg tggccgaggg cgccgagacg ggcgccgggg cggtcgtgac gcacaacgtt
       61 cccgccggcc ggctggtgaa gggcgcgccc gcgcgcgacg ctcgggccgc cggaacgaaa
      121 ccgcgcgagg atggtgattg atggagctcg tcaccaagaa gaagctgacc ttcgtcgccg
      181 ggcgcggcca cgaggaactg tcgtcggaag tcgccagcgc actccaagtg ccggtgggtg
      241 acgtcgtgca ctcgacgttc gcgaacggcg agatctactg ccgctacggc gagaacattc
      301 gcggcgccga cgtgttcgtc ttccagtcgc actgtgatcc gatcaacgac cggatcatgg
      361 aacagctcct gatgatcgac gcggccaagc gcgcgtcggc gaagcgcatc acggcagtca
      421 tcccgtacta cgggtacgcg cgccaagacc gcaaggccga aggtcgcgaa ccgatctccg
      481 cgcggttgct cgccgacatg ttgaccgccg cgggcgcgca gcgcgtcgtc agcgtcgacc
      541 tgcacacggg gcagatccaa ggcttcttcg atttccccgt cgaccacctc actgcggtgc
      601 cggtgctcgc cgcgcacttg gcgaccgaga tcgacagcga gtcggtgatc gtcgctcccg
      661 acgcgggcgg cggcaagctc gcgcgccgct acgccgatcg gctcggcgcc gacatcgcgt
      721 tcatcgacaa gcggcgcccg aagggcacgc acaacgtcgc ggtcgcgacc gaagtcgtcg
      781 gcgacatcga cggccggcag tgcgtgatcg tcgacgacat catcgacacc gcgggcacgg
      841 tcgtcagtgc cgcgaacctg ctgatcgagc ggggcgcgtc ggaggtctac atcgcggcga
      901 cgcacggctt gctgtcgggc ccggccgtcg accggttgaa gaacggcccg atccgcgagg
      961 tcgtcgtcac caacacggtg ccggtgccgt ccgagaagcg cttcgacggc ctccgggtcc
     1021 tgtcgatcgc gccgctgttg gcccgggcga tcgacgccgt gttcgacgac cgctcggtca
     1081 gcgagctctt cggcggcgac aacgtctgac gctcggcgga gccactattt tgggaggggt
     1141 cggcggatgc gcgacaatga tcggtccccg atttcccgca agaggtttga cgcgccatgt
     1201 ccgaagccac gctgaccgcc gacccgcgta ccgagaccgg tagccgcgcc gccggccgtt
     1261 accgccgcga aggcctggtg cccgccgtcg tctacggcct cgagaccgag gcgacctcgg
     1321 tcaccgtgaa cgcccgcgag ctgcagcgca tcctcagcgg cgcgtcgggc gccaacacgt
     1381 tgatcacgct gaaggtcgac ggcggcgaca agctcgcgct ggcccgtcag atccaccggc
     1441 accccgtcaa gggcacgctt gtgcacgtcg acttcgtgct ggtgcgcgcc gaccaggaga
     1501 tcgcggccga cgtcccgatc cacctcgagg gcgaagccga aggcgccaag cgcggcggtc
     1561 tgctcgaaca gctcatgttc tcgatcggca tcaccgcgtt gccgcgtgac atcccgcagt
     1621 cgatcgcgca cgacgtgtcc ggcatggaga tcggcgatca gctgcacgtg agcgacctgg
     1681 tgatccccgc gggtgtcacc gtcacgaacg acctcgacga gctcgtcgtg cagatcactc
     1741 agccgcgcgg tatggaagag gaagaagccg cggaaggcga agccgccgag ggcgaagcgc
     1801 cggccgaagg tgcggcaccc gccgcggccg aaagctccga cagcggggag taacacgtgg
     1861 ttgccgacct gttggtggtc ggcctcggca atccgggggc ggagtacgca cgcacgcggc
     1921 acaacctcgg cgcggaagtc gtcgagatcc tcgcgaaacg ccatggcgcg cggttgcgca
     1981 agacgaagga ccgcgcgctc gtcgacgaca tccgcctcgg cggcaagcag atcgtgctcg
     2041 cggtcccgac gacgtacatg aacgactccg gcgtcgcgat cggcgccatg gtgaagcggt
//
LOCUS       DASUGA010000839         2418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1770183, whole genome shotgun sequence.
ACCESSION   DASUGA010000839 DASUGA010000000
VERSION     DASUGA010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1770183"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="VFX21_16585"
     CDS             <1..1192
                     /locus_tag="VFX21_16585"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX5097643.1"
                     /translation="NPYPMPPGADMFGALLTSEAATQLGYHPYRAPTGVNSVPYDGRP
                     ACNNCGFCGHFGCPIEAKGDPVAPLRNALRTGRCDIRTDSIVERVLLDSSGKRATGVR
                     YLDAAGNAHEVSGNVVIVAGGAWETPRLLLRSDIANSSGLVGRYLMYHFQTFVVGSFP
                     FRLHAHRGRAVTHLHDDHMLVDDDVRSFARECGLPYFRGGLVEHGGGGHPIMEATFTP
                     PGEMHSRTMTDSPMRDRLWAFTMQGEDVPQITNRIDLDPKIRDVHGFAAGRATWDVHR
                     HELAAYRYYAPKLDEIMRAAGAEVTFTASSPRLDGDRPSTEGASQSRHIMGTCRMGND
                     PRTSVVDRWQRFHDVENMVCTDSSVFPTSAGYGPTLTLVALAIRACRNLAGLEPLRSA
                     RPTA"
     gene            1203..1730
                     /locus_tag="VFX21_16590"
     CDS             1203..1730
                     /locus_tag="VFX21_16590"
                     /inference="COORDINATES: protein motif:HMM:NF025279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HEX5097644.1"
                     /translation="MDATKLANVKLADGTGAQHRLGDYWQEQPVVLVFLRHFGCLHCR
                     EHAALLGQRYQDFTAAGVQVVAIGTGDERYAAAFVRDENIPYPVLVDDHAEAADAASV
                     ETVSWGKLLHPNTWKATVATWRNGHKIHKAGKRVKQLGATFVIAPGDHVVYEHLDSDS
                     TDHALADEVLAALPA"
     gene            1735..>2418
                     /locus_tag="VFX21_16595"
     CDS             1735..>2418
                     /locus_tag="VFX21_16595"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5097645.1"
                     /translation="MIKAVVFDFYGTLAETRDWGPSWEQLVAEMGYELDPEIRDRWWN
                     DGIDGTEHDEHSQSRDHYVAWQQARVRAMLNDADIPPATQDQLIERVREISGHRQIHA
                     YDEVADVLGALRERGLALAVCSNWDWDLVEAIESAGLTDTTDFVVSSAWVGARKPHPR
                     IYAHTLEQLGVAPESVLFVGDTWSCDVEGPTAAGMGAVYVRRAHFGVDTTAPEDPSAH
                     PTARRASDLR"
BASE COUNT          410 a          865 c          786 g          357 t
ORIGIN      
        1 caatccgtac ccaatgccgc ccggggccga catgttcggc gcgctcctca ccagcgaggc
       61 cgcgacccaa ctcgggtacc acccataccg cgcgccgacc ggcgtgaact cggtgcccta
      121 cgacggccgg cccgcgtgca acaactgcgg gttctgcgga cacttcgggt gccctatcga
      181 ggccaaaggc gatccggttg cgccgctgcg taatgcactc cgcacgggac gctgcgacat
      241 tcggaccgat tcgatcgtgg agcgggtgct gctcgactcg agcggcaagc gcgcgacggg
      301 cgtgcggtac ctcgatgccg cggggaacgc gcacgaggtt tccggaaacg tggtgatcgt
      361 cgccggcggc gcatgggaga cgccgcggct gctgctgcga tcggacatcg ccaactcgtc
      421 gggcctcgtc ggccgttacc tgatgtacca cttccagacg ttcgtggtcg gctcgttccc
      481 gttccggctg cacgcgcacc gagggcgcgc ggtgacgcat ttgcacgacg atcacatgct
      541 cgtcgacgac gatgtgcgct cgttcgcacg tgaatgcgga ctcccctact tccggggcgg
      601 gctcgtcgag cacggcggcg gcggacatcc gatcatggag gcgacgttca cgccgcccgg
      661 cgaaatgcat tcgcgcacaa tgaccgactc gccgatgcgc gaccggctct gggcattcac
      721 gatgcagggc gaagacgtgc cgcaaatcac gaaccgcatc gacctcgacc ccaagattcg
      781 ggacgtgcac gggttcgcgg ccggccgggc aacgtgggac gtgcaccgcc acgaactcgc
      841 cgcgtatcgc tactacgcgc ccaagctcga cgagatcatg cgcgccgccg gggccgaggt
      901 cacattcacg gcgtcctcgc cccgcctcga cggcgaccgc ccgtccaccg aaggcgcatc
      961 gcagtcgcgt cacatcatgg gaacgtgccg gatgggcaac gacccgcgca ccagcgtggt
     1021 cgaccgctgg caacgcttcc acgacgtcga aaacatggtg tgcaccgact cgtcggtgtt
     1081 cccgaccagc gccggctacg gcccgaccct cacgctcgtc gcgctcgcga tccgggcctg
     1141 ccgcaacctg gccggcctcg agccgctccg cagcgcgcgc ccgactgcgt aggctggccg
     1201 cgatggacgc aaccaaactg gcgaacgtga aactcgccga cggcaccggc gcgcaacacc
     1261 ggctcggcga ctactggcag gagcagcccg tcgtgctggt gttcctgcgg cacttcggct
     1321 gcctgcattg ccgcgaacac gcggcactgc tcggccagcg gtatcaggac ttcaccgccg
     1381 ccggtgtgca ggtggtcgcg atcggcactg gtgacgagcg ctacgccgcc gcattcgtac
     1441 gcgacgagaa cattccgtat ccggtgctcg tcgacgacca cgccgaagct gccgacgcgg
     1501 cgtcggtcga gacagtgtcg tggggcaagc tgctgcatcc gaacacgtgg aaggcaacgg
     1561 tcgcgacgtg gcgtaacggc cacaagatcc acaaggccgg caagcgggtg aagcagctcg
     1621 gcgcgacgtt cgtgatcgct cccggcgacc acgtcgtcta cgagcatctc gactccgaca
     1681 gcacggatca cgcgctggcc gacgaagtgc tcgccgcgct gcccgcgtga ctccgtgatc
     1741 aaggccgtcg tcttcgactt ctacggcacg ctcgccgaga ctcgcgactg gggcccgtct
     1801 tgggagcaac tcgtcgcgga gatgggatac gaactcgatc ccgaaattcg cgaccggtgg
     1861 tggaacgacg ggatcgacgg caccgagcac gacgagcact cgcagtcgcg cgatcactac
     1921 gtcgcctggc agcaagcacg ggtgcgcgcg atgttgaacg acgctgacat cccgcccgcc
     1981 acccaggacc agctcatcga acgagtacgc gagatcagcg gccaccgcca gattcacgcg
     2041 tacgacgaag tcgccgacgt cttgggcgcg ttgcgcgaac gcgggctcgc gctcgcggtg
     2101 tgttccaact gggactggga cctcgtcgaa gcgattgagt ccgccgggct caccgacaca
     2161 accgacttcg tcgtgtcatc ggcgtgggtc ggcgcccgca agccgcatcc gcgcatctac
     2221 gcgcacacgc tcgagcagct cggcgtcgcg cccgagtcgg tgctcttcgt cggcgatacc
     2281 tggtcgtgcg atgtggaggg ccccaccgcg gcggggatgg gcgcggtcta cgtccgtcgc
     2341 gcccacttcg gcgtcgacac caccgccccc gaagacccaa gcgcccaccc caccgcccgc
     2401 cgcgcctccg acctcaga
//
LOCUS       DASUGA010000840         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2012377, whole genome shotgun sequence.
ACCESSION   DASUGA010000840 DASUGA010000000
VERSION     DASUGA010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2012377"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VFX21_16600"
     CDS             <1..787
                     /locus_tag="VFX21_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009399167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase"
                     /protein_id="HEX5097646.1"
                     /translation="SPEGNFIQPSGLMGPGQMFAVLAQRHMHVYGTTREHFCEVAIST
                     RNNALTRANALMKEPLTPEQYFGARMISDPLCLYDFCLECDGAVAVVTTSAERARDLR
                     HPPVTVMASANGGHGRWGQAITWMGMPDEYFASSGHRPVARHLYEMAGVGPADVDVAL
                     LYDHFSPMVLMQLEDYGFCGIGESGPFVADGNIRWPGGSLPVNTHGGNLSEAYIIGMT
                     HVVEAVEQLRGTAVNQVDGAEIALVTGGPASIPVSSLMLRRSA"
     gene            784..1209
                     /locus_tag="VFX21_16605"
     CDS             784..1209
                     /locus_tag="VFX21_16605"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEX5097647.1"
                     /translation="MTPQGVPPADIIWLTPNVFTEPFWLATAQHRLVLPRCTACGTFR
                     FPPSPFCYACRAQAVEWVEHDGNGTVYSFTVIRHAVIPDVKDALPFIAAVVELPDTNG
                     CRIIGDIVGVDANDVRIGMDVRIDWYDVREGTTIPIFRL"
     gene            1371..2276
                     /locus_tag="VFX21_16610"
     CDS             1371..2276
                     /locus_tag="VFX21_16610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097648.1"
                     /translation="MDRISRRALALAGVVTILLSFCSFFAIAASASGNPNPDCPADTI
                     EFSTGSNPIQGLAVGGQKQYVSDGETFTFTKVDDVTLNWSSTIPVSLVYMKGGPTAQL
                     YTYDPPATSGSGLTPAINPNNNQPYGISHVVMCIPDTWSSTTTSTTLASTTTTSTTVA
                     PTTTSTTLEPTTTSTTVAPTTTSTTMEPTTTTSTTVEPTTTSTTAEPTTTTSTTIAVL
                     GSTSTTVEPTTTTVAASGSTSTTLVAAGDTGVTTTTVAPSAPASGASTPALPFTGSNL
                     VLAAFGVLALAAGGACLVAASRRRV"
BASE COUNT          393 a          795 c          736 g          372 t
ORIGIN      
        1 gtcgccggag ggcaacttca tccagccgtc ggggctcatg gggcccggcc agatgttcgc
       61 ggtgctcgcg caacgccaca tgcacgtgta cggcacgacc cgcgagcact tctgcgaagt
      121 cgcgatctcg acgcgcaaca acgcgctgac gcgcgcgaac gcgctgatga aggaaccgtt
      181 gacgcccgag cagtacttcg gtgcgcgcat gatctcggat ccgttgtgcc tctacgactt
      241 ctgtctcgag tgcgacggcg cagtggccgt ggtgacgacg agtgccgaac gagcgcgtga
      301 tctgcgccac ccgccggtca ccgtgatggc gtccgcgaac ggcggccacg gacgatgggg
      361 ccaggcgatc acgtggatgg gcatgcccga cgagtacttc gcgtcgtcgg gtcaccggcc
      421 ggtcgcacgg catctctacg agatggccgg cgtcggcccg gccgacgtcg acgtcgcgtt
      481 gctctacgac cacttcagcc cgatggtgct catgcagctc gaggactacg gcttctgcgg
      541 catcggcgag agcgggccgt tcgtcgccga cggcaacatc cgctggcccg gtgggtcgct
      601 gccggtgaac acgcacggcg ggaacctgtc ggaggcctac atcatcggga tgacgcacgt
      661 cgtcgaagcg gtcgagcaac tgcgaggcac cgccgtcaac caagtcgacg gtgcggagat
      721 cgcgctcgtc accggcggcc ccgcatccat ccccgtttct tctctgatgc tgcggaggtc
      781 cgcatgaccc ctcaaggtgt gccgcccgcg gacatcatct ggctcacgcc gaacgtcttc
      841 accgagccgt tctggctcgc gaccgcgcag caccgtctcg tgctcccgcg gtgcactgcg
      901 tgcggcacgt tccgattccc gccgtcaccc ttttgttacg cgtgccgcgc ccaagccgtc
      961 gagtgggtcg aacacgacgg caatggcacc gtctacagct tcacggtgat tcgccatgcg
     1021 gtcatccccg atgtgaagga cgcgttgccg ttcatcgcgg cggttgtcga attgcccgat
     1081 acgaacggtt gccgcatcat cggcgacatc gtcggcgtcg acgcgaacga cgtgcggatc
     1141 ggaatggacg ttcggatcga ttggtacgac gtgcgcgaag gtaccacgat cccgatcttt
     1201 cgtttgtgaa attcgtcgca tcgccgccat tctgttcaag tttctttgcg cgcgtttgct
     1261 agaagaaaag tcgcgaaccg cgcgtaagcg ggaagcagtt cgcattgtca ggtgggggac
     1321 acacaccaag gacgggccgc tcgcggtcca aggaaaggaa gccccccacc atggaccgca
     1381 tctcacggcg tgcgctggct ctggccggcg tcgtcacgat tctcctgagc ttctgctcgt
     1441 tcttcgcaat tgccgcgagc gccagcggga atcccaaccc tgactgcccg gcggacacga
     1501 tcgagttctc gaccggctcc aacccgatcc agggtctcgc ggtcggcggc cagaagcagt
     1561 acgtgtccga cggcgagacg ttcacgttca cgaaggtgga cgacgtcacg ctcaactggt
     1621 cgtcgacgat cccggtgagc ctcgtctaca tgaagggcgg tccgaccgcg cagctctaca
     1681 cgtacgaccc tcccgcgacg agcggcagcg gcctgacgcc ggcgatcaac ccgaacaaca
     1741 accagccgta cggcatcagc cacgtcgtca tgtgcattcc cgacacgtgg tcgtcgacga
     1801 cgacgtcgac cacgctggca tcgacgacga cgacgtcgac gacggtcgcg ccgacgacca
     1861 cgtcgaccac gctggagccg acgacgacgt cgacgacggt cgcgccgacc acgacgtcga
     1921 cgaccatgga accgacgacc acgacgtcga cgacggtaga gccgacgacg acgtccacca
     1981 cggcggagcc cacgaccacg acgtcgacca cgatcgccgt cctcggcagc acttcgacga
     2041 ccgtcgagcc gacgacgaca actgtcgctg cgtcgggttc gacgtcgacg acgctcgtcg
     2101 ccgcgggcga caccggcgtg acgacgacga cggtcgcacc gagcgcgccg gcatccggcg
     2161 cgagcacgcc ggcgttgccg ttcaccggct cgaacctcgt tctcgcggca ttcggtgtct
     2221 tggcgctggc ggccggcggg gcgtgcctcg tcgccgcgtc gcgccggcgc gtctgagccc
     2281 gcgaccccgc gcgcac
//
LOCUS       DASUGA010000841         2860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_937232, whole genome shotgun sequence.
ACCESSION   DASUGA010000841 DASUGA010000000
VERSION     DASUGA010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937232"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2076)
                     /locus_tag="VFX21_16615"
     CDS             complement(<1..2076)
                     /locus_tag="VFX21_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HEX5097649.1"
                     /translation="MRMLERPGEIDLEEAIADLRTAHISRFLDDREIALRQQNKAFFQ
                     ISGAGHEALLLGLARSLRPGYDWFFPYYRDRALALALGVTPLEILLQAVGADDDPASG
                     GRQMPCHWGARELNIVTQTSCTGSQCLPAVGSAEAARYIGRRPDLPGCSAHGDELTYV
                     SLGEGATSEGEFWESLNTACRLHLPLLYVVADNGYAISVRSTDQMPAPISEMVRGIRG
                     LHIVKMDGRDYFEVRRRASDAVARVRAGAGPTLVHATVTRPYSHSLSDDQKKYRGSTE
                     LQDEAEHDPILVLANHLVAMGALTQEQADAIRDEAKETVRAAAEAALAAPRPEPISVT
                     RNLFGPPPVSEDPGEPEVSADSEVVTYGEAIRRTLHEEMARDERIRVFGEDVADADPE
                     VINEVPGKGGVFGITFGLQRTFGEARCFNTPLAEANIIGRAVGQAMRGLRPCPEIQFF
                     DYVWPAMNQIKSEAATTRWRSNGAFSCPMVVRIPIGGYLQGGAIWHSQCGESIFAHVP
                     GLLIAMPSRARDAAGLLRTALRSEDPVLFLEHKHLYRQGYNRDPMPPADWMLPFGKGA
                     YVRRGDRVTVVTWGATVHRAKQAAQQLGEGAGVEIVDLRTISPWDRDIVAESVKKTHR
                     VLVVHEDTLTCGFGAEVAAFVADECFEALDAPVWRLAATDTPVAYEPTLEDAILPQIA
                     DIARDLQALL"
     gene            2241..>2860
                     /locus_tag="VFX21_16620"
     CDS             2241..>2860
                     /locus_tag="VFX21_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX5097650.1"
                     /translation="MADAEEILPWRVRRIRQVAGFLSPAARRAAAALGIDARGMTGTG
                     DGGRVTLTDVQAGANAAPRGDEVVPFNTVRKRAATLLVASKHTSAHTLVVRRADYTRI
                     EEARSGAGLTALAFVARAVVDALHQFPHMNAGVDGDALVLHRAVNLGIAVDLNYEGLV
                     VPVIRDADGLRLRALATSIRDVAAKARAKRLLPDDFAGGTFTITNPG"
BASE COUNT          406 a          981 c         1012 g          461 t
ORIGIN      
        1 gaggagggct tggaggtcac gggcgatatc ggcgatctgc gggaggatcg cgtcttcgag
       61 cgtcggctcg tacgcgacgg gggtgtcggt ggcggcgagg cgccacaccg gcgcgtcgag
      121 cgcttcgaag cactcgtcgg cgacgaacgc tgccacttcg gcgccgaagc cgcacgtgag
      181 cgtgtcctcg tggacgacca acacgcggtg cgtcttcttc accgactcgg cgacgatgtc
      241 gcggtcccac ggtgagatcg tgcgcaggtc gacgatctcg acgccggccc cttcgcccag
      301 ttgctgcgcg gcctgcttgg cgcgatgcac ggtcgcgccc cacgtgacga cggtgacgcg
      361 gtcgccgcgc cgcacgtacg cgcccttgcc gaacgggagc atccagtctg cgggcggcat
      421 cgggtcgcgg ttgtaaccct gccggtacaa gtgcttgtgc tcgaggaaca acaccgggtc
      481 ctcgctgcgc agcgcggtgc gcaacagccc ggccgcgtcg cgcgcccgcg acggcatggc
      541 gatcaacagg ccgggcacgt gggcgaagat cgactcgccg cactggctgt gccagatcgc
      601 gccgccctgc aaatagccgc cgatcgggat ccgcaccacc atcgggcacg agaacgcacc
      661 gttcgagcgc cagcgtgtcg tcgccgcctc cgacttgatc tggttcatcg cgggccacac
      721 gtagtcgaag aactggatct cggggcacgg tcgcagcccg cgcatcgcct gcccgaccgc
      781 gcggccgatg atgttcgcct ccgcgagcgg cgtgttgaaa cagcgcgcct caccgaaggt
      841 gcgctgcaag ccgaacgtga tgccgaacac gccacccttg cccggcactt cgttgatgac
      901 ttcgggatcg gcgtcggcga cgtcttcgcc gaacacgcgg atgcgttcgt cgcgcgccat
      961 ctcctcgtgc agtgtgcggc gtatcgcttc gccgtaggtg acgacctcgc tgtccgcgct
     1021 gacctcgggt tcgcccgggt cctcgctcac cggcggcggg ccgaacagat tgcgcgtcac
     1081 cgagatgggt tcgggacggg gcgccgcgag cgcggcctcg gccgcggcgc gcacggtctc
     1141 cttggcctcg tcgcggatcg cgtcggcctg ctcctgcgtc agcgcgccca tggcgacgag
     1201 atgattggcg agcacgagga tcgggtcgtg ctcggcttcg tcctgcagct ccgtcgaacc
     1261 gcggtacttc ttctggtcgt cggagagtga gtgcgagtac ggacgcgtga ccgtggcgtg
     1321 cacgagtgtc ggtcccgcgc ccgcccgcac tcgcgccacc gcgtcggacg cgcgccgccg
     1381 gacttcgaag tagtcgcggc cgtccatctt cacgatgtgc aggccgcgga tgccgcgcac
     1441 catctccgag atcggggccg gcatctggtc ggtcgagcgc acggagatcg cgtatccgtt
     1501 gtcggcgacg acgtagagca gcggcaggtg caaccggcac gcggtgttga gcgactccca
     1561 gaactcgcct tccgacgtcg cgccctcgcc gagcgagaca tacgtcagct cgtcaccgtg
     1621 cgcggagcac ccgggcagat cggggcggcg gccgatgtag cgcgcggcct cggcggaccc
     1681 gaccgcgggc aggcactggc tgccggtgca cgacgtttgc gtgacgatgt tcagctcgcg
     1741 cgcgccccag tggcacggca tctgccgccc gcccgacgcg gggtcgtcgt cggcgccgac
     1801 tgcttgcaac aggatctcga gcggtgtgac gccgagcgcg agggcgagcg cccggtcgcg
     1861 gtagtagggg aagaaccagt cgtagccggg gcgcagggag cgcgcgagcc cgaggagcaa
     1921 tgcttcgtgg ccggccccgg agatctggaa gaacgccttg ttctgctggc gcagcgcgat
     1981 ctcgcggtcg tcgaggaagc gggagatgtg cgccgtgcgc aggtcggcga ttgcttcttc
     2041 gaggtcgatc tctcccggac gttcgagcat gcgcatgagc ccgcacacct tctttctttg
     2101 tcgctctcga cctgctcgcg tgagacggct cggcctgttg gccggcctcg agactccgcg
     2161 agtgccttcg cgagctccgg caccgcgctt ctcggggacg gctccgtctc ctccaagtta
     2221 cgacctaccc tcggtactca atggcggatg ctgaagagat ccttccctgg cgggtccgcc
     2281 gcatacgtca ggtggcgggg ttcctgtcac cggcggcccg ccgcgccgcg gcggcgttgg
     2341 gcatcgatgc ccgcgggatg accgggaccg gcgacggtgg acgcgtcacc ctcaccgacg
     2401 tgcaagcggg cgcgaatgcg gcaccgcggg gcgacgaggt cgtgccgttc aacaccgtgc
     2461 gcaagcgcgc cgcgacgttg ttggtggcgt cgaagcacac gtccgcgcac acgctggtcg
     2521 tccggcgcgc cgactacaca cgcatcgagg aggcacgttc cggcgcgggg ctcaccgcgc
     2581 tcgcgttcgt cgcgcgtgca gtcgtcgacg cgttgcacca gtttccacac atgaacgcag
     2641 gcgtcgacgg cgacgcgctc gtgttgcacc gagccgtgaa cctcggcatt gctgtcgacc
     2701 tgaactacga gggactcgtc gtaccggtga tccgtgacgc cgacggcttg cggctccggg
     2761 cgctggcgac gtcgatccgc gatgtcgcgg cgaaggcgcg ggccaagcgt ttgctgcccg
     2821 acgacttcgc gggcggcacg ttcacgatca ccaatccggg
//
LOCUS       DASUGA010000842         2132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1075291, whole genome shotgun sequence.
ACCESSION   DASUGA010000842 DASUGA010000000
VERSION     DASUGA010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075291"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VFX21_16625"
     CDS             complement(<1..321)
                     /locus_tag="VFX21_16625"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5097651.1"
                     /translation="MADVIERRKVGRPARLDRDMIAKAAHEVGLDRVTMKAVADRLGV
                     SVPGLYHHIEGRDDLMRLAAEYSASRIPLPVDRGQHWAAWLLEWAQYSHDAFVAQPEL
                     LGQFL"
     gene            393..1262
                     /locus_tag="VFX21_16630"
     CDS             393..1262
                     /locus_tag="VFX21_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016468119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5097652.1"
                     /translation="MDSLREKVAVVTGGASGIGFALARAFLGEGMRVVVADVEEGALE
                     RAAKELDGLGELRTVRTDVTEPASVDTLADRTYDEFGACHVLCNNAGVGAPSANIWET
                     TVNDWKWVHGVNVMGVVHGILSFVPRMIASGEEGFVCNTSSGDGGIAPLPQASVYASS
                     KAAVTCLTECLAAQLESENTKLRAGIFYPSGGLLRTGLWTADRNRPADLAREKPRPTP
                     AMTIETLEKMAKDAGRELPFQDLDELAQMVVGGIKEDRFVMMLGIESIGTQMRDRAGA
                     LEQGARPTAHQFG"
     gene            complement(1322..>2132)
                     /locus_tag="VFX21_16635"
     CDS             complement(1322..>2132)
                     /locus_tag="VFX21_16635"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5097653.1"
                     /translation="NGCVALVLSEHHAVDDGYLPSPMVMAAAMAARTTTTPIMIAAAL
                     LPFYEPVRLAEDMSVLHHLSNGRVSYVFGLGYRPDEFALFGLDMRARARIADTKLEQL
                     LDALERVTPRAEPRPAISWGGGTPAAARRAARFGLDFLAQTDGDDLGAAYRDECAQQG
                     REPGNCMLANPDDPVTVFVHDDLDTAWDELGPYLLHDARSYSSWNVNPRVASISRGET
                     VDALRKENGAHRIMTSADAKARGFLPLHPLCGGVPPEMAWPYLERAAAAFR"
BASE COUNT          335 a          692 c          782 g          323 t
ORIGIN      
        1 gaggaactga ccgagcaact cgggctgggc gacgaacgcg tcgtgcgagt actgcgccca
       61 ctcgagcaac cacgcggccc agtgctgccc acggtcgaca gggagtggga tgcgcgacgc
      121 ggagtactcg gccgcgaggc gcatgagatc gtcgcggccc tcgatgtggt ggtagaggcc
      181 gggcacgctc acgccgaggc ggtcggcgac cgccttcatc gtcacccggt cgaggccgac
      241 ctcgtgggcg gccttggcga tcatgtcgcg atccaggcgc gcgggccggc cgaccttgcg
      301 ccgttcgatc acgtccgcca tggctgacct ttcgagcgag ttttcggaac tgtacttgaa
      361 aatagatccg ccgacgcaca aggatcgcac gtatggactc gttgcgggaa aaggtcgccg
      421 tcgtcaccgg tggtgcgtcg ggtatcgggt tcgccctggc ccgggcgttc ctcggcgagg
      481 gcatgcgggt agtcgtcgcc gacgtcgagg agggcgcgct cgaacgggcc gccaaggagc
      541 tcgacggtct cggcgagctg cgcaccgtgc gcaccgacgt caccgagccg gcgtcggtcg
      601 acacgttggc cgaccgcacg tacgacgagt tcggcgcgtg ccacgtgttg tgcaacaacg
      661 cgggcgtcgg cgcgccctcg gcgaacatct gggagacgac ggtcaacgac tggaagtggg
      721 tgcacggcgt caacgtgatg ggcgtcgtcc acggcatcct gtcgttcgtg ccgcggatga
      781 tcgcgagcgg cgaagagggg ttcgtgtgca acacgtcgtc gggtgacggc ggcatcgcgc
      841 cactcccgca agcgagcgtg tacgcgtcga gcaaggccgc ggtgacgtgt ctcaccgaat
      901 gcctcgccgc gcagctggag tccgagaaca cgaagctgcg cgcgggcatc ttctatccct
      961 cgggcggctt gttgcggacc ggcctgtgga ctgcagatcg caaccggccc gcggacttgg
     1021 cgcgggagaa gccgcggccg acgcccgcga tgacgatcga gacgctcgag aagatggcca
     1081 aggacgccgg gcgcgagctc ccgttccagg acctcgacga gctcgcgcag atggtcgtcg
     1141 ggggcatcaa ggaagaccgc ttcgtgatga tgctcggcat cgagtcgatc ggcacccaga
     1201 tgcgcgaccg cgcgggcgca ctggagcagg gcgcccgccc gaccgcacac cagttcggct
     1261 gaaccgatgc tcgggcgtct tacaagggcg gattccgccc ttgtaagacg cccagggtcg
     1321 tttagcggaa cgcggcggct gctcgctcga ggtacggcca cgccatctcg ggtggcacgc
     1381 ccccgcagag cggatgcagt ggcaggaacc cgcgcgcctt cgcgtccgcg ctcgtcatga
     1441 tccggtgcgc gccgttctct ttgcggagcg cgtcgaccgt ctcgcctcgg gagatgctcg
     1501 caacgcgcgg gttgacgttc cacgacgaat agctgcgcgc gtcgtgcagt aggtagggac
     1561 cgagctcgtc ccacgccgtg tcgaggtcgt cgtggacgaa caccgtgacg gggtcgtcgg
     1621 ggttcgcgag catgcagttg cccggctcgc gcccttgctg cgcgcattcg tcgcggtagg
     1681 cggcgccgag atcgtcgccg tcggtctgcg cgaggaagtc gagaccgaag cgcgcggccc
     1741 gacgggccgc tgcgggcgtg ccaccacccc acgagatcgc gggccggggc tcggcgcggg
     1801 gcgtgacccg ctcgagggcg tcgaggagtt gctcgagctt ggtgtccgcg atgcgggcgc
     1861 gtgcacgcat gtcgaggccg aagagcgcga actcgtcggg gcggtaaccg aggccgaaca
     1921 cgtacgacac ccggccgttg ctcaggtgat gcagcaccga catgtcctcg gccaggcgca
     1981 ccggctcgta gaacggcaac agcgcggccg cgatcatgat cggtgtcgtc gtcgtgcgcg
     2041 ccgccatcgc tgcggccatg accatgggtg acggcaggta gccgtcgtcg acggcgtgat
     2101 gctccgagag cacgagggca acgcacccgt tc
//
LOCUS       DASUGA010000843         4623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_520759, whole genome shotgun sequence.
ACCESSION   DASUGA010000843 DASUGA010000000
VERSION     DASUGA010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520759"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VFX21_16640"
     CDS             <1..325
                     /locus_tag="VFX21_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEX5097654.1"
                     /translation="DEVRLQPTVEWMLAGLPWMCAEQLAFMDRKVALTLTGPGGGTWL
                     IAPGGVDGRVTVTEGKDGAAATLTSTTHDFVIWGTQRRPWRTLATVVGDEEYATRVFD
                     AVNII"
     gene            449..1336
                     /locus_tag="VFX21_16645"
     CDS             449..1336
                     /locus_tag="VFX21_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097655.1"
                     /translation="MFRGIFVATCALAVAVVGIASPAAAKKAAASPWGTSFVDRDNNG
                     VFSTGDQDLATVLAANGGFYNHTSGARESIVLENVALTEKEPFYFVNVNGNITVRGNY
                     TVKGADGMITFSTVRGDIAIPAGAKVLSKGNLSLQTYSGNMSIGANAQISGSGDFWDT
                     SFTGTTASIGDNVKFSAGGGYHDVTVRGNNSLTVGKNISATGPGHGGFRLQTDGDLTI
                     DRINVRLGYIYVLTGHDGIQSRLHISNSTFNQTYRNGAAIFSSSNNGAYAPDAIVFTN
                     VTITSKGGDVRTYPQPIIK"
     gene            complement(1350..2378)
                     /gene="ilvC"
                     /locus_tag="VFX21_16650"
     CDS             complement(1350..2378)
                     /gene="ilvC"
                     /locus_tag="VFX21_16650"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX5097656.1"
                     /translation="MPATVYYDNDADTGLIADKKVAILGYGSQGHAHALNLKESGIDV
                     IVGLREGSASKAKAEAAGLRVLPTGEAVREADIVMVLLPDTEQKKVYDADIAPNLVDG
                     NSIAFAHGFNIHFGQITPPAGVDVWMIAPKGPGHLVRRTYEEGGGVPCLVAIHNDATG
                     KAKQTALAYAKAIGGTRAGVLDTTFEEETETDLFGEQVVLCGGLVELIRNGYETLVEA
                     GYQPESAYFETLHEVKLIVDLIYEGGIASMNYSISDTAEYGELSRGPRVVTPATKTEM
                     KKILGEIQSGSFAKEWIAENENGRPNFNKMREQIAQHPIEKVGAELRSMMPWISAGKA
                     KPQDVSGG"
     gene            complement(2412..2942)
                     /gene="ilvN"
                     /locus_tag="VFX21_16655"
     CDS             complement(2412..2942)
                     /gene="ilvN"
                     /locus_tag="VFX21_16655"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX5097657.1"
                     /translation="MNGVPHHILTVLVENKPGVLSRVAGLFSRRAFNIFSLAVSPTDD
                     ERFSRITMVVDAESAPLEQVVKQLNKLIPVIKITELDPLGGVERELMMVTVKAGSEAR
                     SQVTELTAIFEAKIVDVGYDAMTIMVAGEPDKLDAFSDLVKPFNIVELQRTGRIALPK
                     LSRQSTRLRAVKTARA"
     gene            complement(2945..>4623)
                     /locus_tag="VFX21_16660"
     CDS             complement(2945..>4623)
                     /locus_tag="VFX21_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEX5097658.1"
                     /translation="EQVEVMFGLPGGAILPVYDPLIDSSIRHILVRHEQGAGHMASGY
                     AHATGRPGVAMVTSGPAATNIVTPLCDAYMDSVPFVCITGQVAYSVIGTDAFQEADTV
                     GITMPVTKHNWLVSDAQDIPRIVREAFHVATSGRPGPVLVDFPKDIANATMEWYWPEK
                     VDLPGYKPNVKGHPRQIKDAARLIGEAQRPVIYAGGGILKAFASQELQEFAELTGIPV
                     VTTLMARGALPDEHPLCLGMPGMHGNYTAITSLQKSDLLVTLGARFDDRVTGNPATFA
                     ANAKVIHVDIDPAELGKVRRPDVPIVGDCKVVIGELIKAVKHDREKHEAPDLSAWIAQ
                     LREWQERFPLTYKQEEGGPLKPQFCIEALRDNTPDDTILVSGVGQHQMWASQLWKFRR
                     PNTWINSGGLGTMGYAVPAAVGAKVGRPDRMVWAVDGDGCFQMTAQELVTAAAERIPI
                     KVAILNNAYLGMVRQWQELFYEERYSEVYLSPDLPDYVKWAEAMGCVGMRVDSADDVV
                     TTIEKANEIDDRPVVIDFRTDYREKVYPMVPAGASNDDVILGPEFGEGGR"
BASE COUNT          753 a         1510 c         1539 g          821 t
ORIGIN      
        1 cgacgaagtg cgcctgcagc cgacggtcga gtggatgctc gcggggctgc cgtggatgtg
       61 cgcggagcaa ctggcgttca tggaccgcaa ggtcgcgctc acgttgaccg gtcccggtgg
      121 tggtacatgg ctgatcgcac ccggaggcgt cgacgggcgc gtcaccgtca ccgaaggcaa
      181 ggacggcgcg gcggcgacgc tgacgtcgac gacgcacgac ttcgtcatct ggggcacgca
      241 acgacgcccg tggcgcaccc tcgcaacggt cgtcggcgac gaggagtacg cgacgcgcgt
      301 cttcgatgcg gtcaacatca tctgaagtgc aacacctggt tacattttcc agttcgtttg
      361 gatcaggaaa ctgacgtaac gtgttcctac gattgcgctg cgcgcgggcc gataaacggg
      421 gaaggcctgc aaccgtgggg gaaccggcat gttcagggga atcttcgtcg cgacgtgcgc
      481 gctggccgtc gccgtcgtgg gaatcgcgtc gccggcggcg gccaagaagg ccgccgcatc
      541 accgtggggt acgtcgttcg tcgatcgcga caacaacggc gtgttcagca cgggcgatca
      601 ggatctggcg accgtgctcg ccgcgaacgg tggcttctac aaccacacga gcggcgcccg
      661 cgagagcatc gtgctcgaga acgtcgcgct gacggagaag gagccgttct acttcgtgaa
      721 cgtcaacggc aacatcacgg tgcgcggcaa ctacaccgtg aagggcgcgg acgggatgat
      781 cacgttctcg accgtgcggg gcgacatcgc catcccggcc ggcgcgaagg tcctgagcaa
      841 gggcaacctc tcgctccaga cctacagcgg caacatgagc atcggtgcca acgcgcagat
      901 ctcgggttcc ggcgacttct gggacacttc gttcaccggg acgacggcga gcatcggcga
      961 caacgtgaag ttcagtgccg gcggcggcta tcacgacgtc accgtgcgcg ggaacaactc
     1021 gttgaccgtc ggcaagaaca tctcggcgac cggaccgggg cacggcgggt tccgcttgca
     1081 gaccgatggc gatctcacga tcgaccgcat caacgtgagg ctcggttaca tctatgtgct
     1141 caccgggcac gacggcatcc agagccggct gcacatctcg aactcgacct tcaaccagac
     1201 gtaccgcaac ggcgccgcga tcttctcgag cagcaacaac ggtgcctacg cccccgacgc
     1261 gatcgtgttc acgaacgtca cgatcacgtc gaagggcggc gacgtccgca cgtacccgca
     1321 gccgatcatc aagtagcgcc gagacgcgcc tagcccccgg agacgtcctg gggcttggcc
     1381 ttgccggccg agatccacgg catcatcgaa cgcagctcgg cgccgacctt ctcgatcggg
     1441 tgctgcgcga tctgctcgcg catcttgttg aagttcgggc ggccgttctc gttctcggcg
     1501 atccattcct tcgcgaacga gcccgactgg atctcaccga ggatcttctt catctcggtc
     1561 ttcgtcgcgg gcgtgacgac acgcgggccg cgtgacagct cgccgtactc ggcggtgtcg
     1621 gagatcgagt agttcatcga cgcgatgccg ccctcgtaga tgaggtcgac gatgagcttc
     1681 acttcgtgca gcgtctcgaa gtacgcggac tcgggttggt acccggcctc gacgagcgtc
     1741 tcgtagccat tgcgaatgag ctcgacgagc ccgccgcaca acacgacctg ctcaccgaag
     1801 aggtcggtct cggtctcttc ctcgaacgtc gtgtcgagca cgccggcgcg ggtgccgccg
     1861 atcgctttcg cgtacgcgag tgcagtctgc ttcgccttgc cggtcgcgtc gttgtggatc
     1921 gcgacgaggc acggcacacc gccgccctct tcgtaggtgc ggcgcacgag gtgcccgggg
     1981 cccttgggcg cgatcatcca gacgtcgaca ccggcgggcg gtgtgatctg gccgaagtgg
     2041 atgttgaagc cgtgtgcgaa cgcgatcgag ttgccgtcga cgaggttcgg agcgatgtcg
     2101 gcgtcgtaga ccttcttctg ctcggtgtcg gggagcagca ccatgacgat gtcggcctcg
     2161 cggaccgctt cgccggtcgg cagcacgcgc agacccgcgg cctccgcctt cgccttcgac
     2221 gccgacccct cacgcagacc gacgatgacg tcgatccccg actccttcag gttcagcgcg
     2281 tgcgcatggc cctgcgagcc gtagccgagg atcgcgacct tcttgtcggc gatgaggccg
     2341 gtgtcggcgt cgttgtcgta gtacacggtt gcgggcattg cagcgctccc cagttcaggt
     2401 gttcgaagag atcaggcgcg tgcggtcttg acggcccgca ggcgagtgga ctggcgtgac
     2461 agcttgggca gcgcgatgcg gcccgtccgt tgcagctcca cgatattgaa cggcttgacc
     2521 agatcggaaa acgcgtcgag cttgtcgggc tcgccggcga ccatgatcgt catcgcgtcg
     2581 tagccgacgt cgacgatctt ggcctcgaag atcgccgtca gctcggtgac ctggctgcgg
     2641 gcctcggacc cggccttgac ggtcaccatc atcagctccc gctcgacccc gccgagagga
     2701 tcgagctcgg tgatcttgat gaccgggatg agcttgttga gctgcttcac gacctgctcg
     2761 agcggcgcgg actccgcgtc gacgaccatc gtgatgcggc tgaaccgctc gtcgtcggtc
     2821 gggctgaccg cgagcgagaa gatgttgaac gcccgccgcg agaacagccc ggcgacgcgc
     2881 gacagcacgc ccggcttgtt ctcgacgagg acggtgagga tgtggtgcgg cacgccgttc
     2941 atgtctagcg accgccttcg ccgaattcag gaccgaggat gacgtcgtcg ttggacgcgc
     3001 ccgccgggac catcgggtac accttctcgc ggtagtcggt gcggaagtcg atcacgaccg
     3061 gacggtcgtc gatctcgttc gccttctcga tcgtcgtcac gacgtcgtcc gcgctgtcga
     3121 cgcgcatccc gacgcaaccc atcgcctccg cccacttcac gtagtcgggc agatcgggtg
     3181 acaggtacac ctcgctgtag cgctcttcgt agaacagctc ctgccattgg cgcaccatcc
     3241 cgaggtacgc gttgttcaag atcgcgacct tgatcgggat gcgctccgcg gccgcagtga
     3301 cgagctcttg cgcagtcatc tggaagcagc cgtcaccgtc gactgcccac accatgcggt
     3361 cggggcgacc gaccttggcg ccgaccgcgg cgggcaccgc gtaacccatc gtgccgagac
     3421 cgccggagtt gatccacgtg ttcggccggc ggaacttcca cagctggctc gcccacatct
     3481 ggtgctgacc gacacccgac acgaggatcg tgtcgtcggg cgtgttgtcg cgcagcgctt
     3541 cgatgcagaa ctgcggcttg agcgggccac cttcctcttg cttgtacgtg agcgggaagc
     3601 gctcctgcca ctcccggagc tgcgcgatcc acgcgctcag gtcgggtgct tcgtgcttct
     3661 cgcggtcgtg cttcacggcc ttgatcagtt cgccgatgac gaccttgcag tcgccgacga
     3721 tcgggacgtc gggtcgccgc accttgccca gctcggcggg atcgatgtcg acgtggatga
     3781 ccttcgcgtt ggccgcgaac gtcgcagggt tgccggtgac gcggtcgtcg aagcgcgcgc
     3841 cgagcgtcac gagcagatcc gacttctgca gcgacgtgat cgccgtgtag ttcccgtgca
     3901 tgcccggcat gccgaggcac agcggatgtt cgtcgggcag cgcaccgcgc gccatgagcg
     3961 tcgtcacgac cgggatgcct gtcagctccg cgaactcctg cagttcctgc gaggcgaacg
     4021 ccttcaggat gccgccgccc gcgtagatga ccggccgctg tgcttcaccg atcaaacgcg
     4081 ccgcgtcctt gatctgacgc gggtggccct tgacgttcgg cttgtagcca ggaaggtcga
     4141 ccttctcggg ccagtaccac tccatcgtcg cgttggcgat gtccttgggg aagtcgacga
     4201 gcacggggcc cgggcgaccg ctcgtcgcga cgtggaacgc ctcccggacg atgcgcggga
     4261 tgtcctgggc gtcgctcacg agccagttgt gcttcgtgac gggcatggtg atgccgaccg
     4321 tgtcggcttc ctggaacgca tcggtgccga tgacgctgta cgcgacctgg ccggtgatgc
     4381 acacgaacgg cacggagtcc atgtacgcgt cgcacagcgg cgtgacgatg ttcgtcgccg
     4441 cgggaccgct ggtgaccatc gcgacgccgg gccggccggt cgcgtgggcg tagcccgatg
     4501 ccatgtggcc cgcgccctgc tcgtgccgga cgaggatgtg gcggatcgaa gagtcgatga
     4561 gcgggtcgta gacggggagg atcgccccgc cggggaggcc gaacatgacc tcgacctgct
     4621 cca
//
LOCUS       DASUGA010000844         3408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1633464, whole genome shotgun sequence.
ACCESSION   DASUGA010000844 DASUGA010000000
VERSION     DASUGA010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633464"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(276..2522)
                     /locus_tag="VFX21_16665"
     CDS             complement(276..2522)
                     /locus_tag="VFX21_16665"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEX5097659.1"
                     /translation="MPESVTPGTPVDFVESVYARYPERVDIARERLGRPLTFAEKVLI
                     AHADDPRAVGLARGVDYADYRPDRVAMQDATAQMALLQFMLAKLSRAAVPTTVHCDHL
                     IQARLGADADMQVALDTNQEVYEFLRTVSAKYGIGFWKPGSGIIHQVVLENYAFPGGM
                     MIGTDSHTPNAGGLGMVAIGVGGADAVDVMAGWPFNTRVPKLIGVHLTGELSGWAAPK
                     DVILKVAGILTVKGGTGAVVEYFGPGARSISATGKATICNMGAEIGATCSVFAYDDQT
                     AMYMKATGREALADLADRYAEHLRNDPEVDANPEQFYDRVVEIDLSELEPHLVGPHTP
                     DLDRPISDIAAEAEREGYPKEISAALVGSCTNSSYEDIGRAAHIARQAIAQGRRVKAP
                     LMITPGSEQVRATIERDGLLADLEAIGATVLANACGPCIGQWKRDDVQSGQKNTIVTS
                     FNRNFPARNDGNKDTYAFIGSPETVVGMALLGVLDGSFTKEFEAPVADALPSRGFDAG
                     ESGFIAPADDPATVEVAITPGSERLEALTPFPAWDGNDIAGLRVLLRAKGKCTTDHVS
                     PAGPWLKYRGHLTNISGNLFSGAVNVFADREPGQGVDARDGAVTPLPELARTYKEAGI
                     EWLAIGDENYGEGSSREHAALEPRYMNGRAILARSFARIAEANLKKQGLLPLTFANPA
                     DYDKVKADDVVDLLGVDALAPGREIRVVLHHADGSTDEFPAKHSMSEEHIDWFRAGSA
                     LNVLAARA"
     gene            complement(2515..3237)
                     /locus_tag="VFX21_16670"
     CDS             complement(2515..3237)
                     /locus_tag="VFX21_16670"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX5097660.1"
                     /translation="MRYLELADAIRARVAAGDVGPGGALASEAELSRAYGASRVTVRR
                     ALDQLRREGLVVSRRGSGWFAALDPVRQPLGRVTTVEAAVEAAGARPSRRILSFAFVA
                     APTAVAGALGLRDDADVLRVERVNLADDEPFALVTVWVRGDLGAGLSRAHVERAPFYD
                     LLPVRGVSLAHAQQTITAELAAPDHARLLDVDAGSPLLLLRRTTFDVDDAPVLYSEHR
                     YPAGRTTFEIDFSLSAGVLQHA"
     gene            complement(3285..>3408)
                     /locus_tag="VFX21_16675"
     CDS             complement(3285..>3408)
                     /locus_tag="VFX21_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019609702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEX5097661.1"
                     /translation="KSAKWLRGFEFLDHDEPGFWERNGYHNYGDPWREQRYWGD"
BASE COUNT          517 a         1171 c         1188 g          532 t
ORIGIN      
        1 ccggtcgagg aacgcaaaca gctcgacggc gcggccgcgg tgatgccact cgacctcgac
       61 aaggagctcg cccgctacgg ctgaccgacg caacctgcgc ctttgaacca ccggcgcggt
      121 ggtcgcggga cgcaggtttg gagcaccgcg cgtacctccg tctctgaacc acccacgcgg
      181 tggtcgaggg acgcaggttc gcagctcgtt actcggcgaa ctcgacgacg tcggcgcccg
      241 tgcgccggag caactcgtcg aacgggctca caccttcagg ctcgcgcggc cagcacgttg
      301 agtgccgaac cggcccggaa ccagtcgatg tgctcctccg acatcgagtg cttcgcgggg
      361 aactcgtcgg tgctgccgtc ggcgtggtgc agcacgacgc ggatctcacg gccgggcgcg
      421 agcgcgtcga cgccgagcaa gtcgacgacg tcgtcggcct tgaccttgtc gtagtcggcc
      481 gggttcgcga acgtcagcgg cagcaagccc tgcttcttca agttcgcctc ggcgatgcgc
      541 gcaaacgagc gagcgaggat cgcgcggccg ttcatgtagc gcggttcgag cgccgcgtgc
      601 tcacgcgacg atccctcgcc gtagttctcg tcgccgatcg ccagccactc gatacccgcc
      661 tctttgtagg tgcgcgcgag ctcgggcagc ggtgtcaccg caccatcgcg agcgtcgacg
      721 ccctgcccgg gttcgcggtc ggcgaacacg ttgaccgcgc cgctgaacag gttgccggag
      781 atgttcgtga ggtgaccgcg gtacttcagc cacggaccgg ccggtgacac gtggtcggtc
      841 gtgcacttgc ccttcgccct caacagcacg cgcagtccgg cgatgtcgtt gccatcccac
      901 gccgggaacg gcgtgagcgc ttcgagccgc tccgaaccgg gcgtgatcgc aacctcgacc
      961 gtcgccggat cgtcggccgg cgcgatgaag cccgactcgc cggcgtcgaa cccgcggctc
     1021 ggcaacgcgt ccgcgaccgg cgcctcgaac tccttggtga acgacccgtc gagcacaccg
     1081 agcagcgcca tgccgaccac cgtctcgggt gaaccgatga acgcgtatgt gtccttgttg
     1141 ccgtcgttgc gcgccgggaa gttgcggttg aacgacgtga cgatcgtgtt cttctgcccg
     1201 ctctgcacgt cgtcgcgctt ccactggccg atgcaggggc cgcacgcgtt ggcgagcacc
     1261 gtcgcaccga tcgcttcgag gtcggcgagc aggccgtcgc gctcgatcgt ggcgcgcacc
     1321 tgctcggagc ccggcgtgat catcagcggt gccttgacgc gccggccttg cgcgatggct
     1381 tggcgcgcga tgtgcgcggc gcgcccgatg tcttcgtacg acgagttggt gcacgagccg
     1441 acgagcgccg ccgagatctc cttcgggtag ccctcgcgtt cggcttccgc ggcgatgtcg
     1501 gagatcggac ggtcgagatc gggcgtgtgc gggccgacga ggtgcggttc gagctccgac
     1561 aggtcgatct cgacgacacg gtcgtagaac tgctcggggt tcgcgtcgac ctcggggtcg
     1621 ttgcggaggt gctcggcgta gcgatcggcg aggtcggcca gcgcttcgcg ccccgtcgcc
     1681 ttcatgtaca tcgcggtctg gtcgtcgtac gcgaacaccg agcacgtggc gccgatctcc
     1741 gcgcccatgt tgcagatggt cgccttgccg gtcgcgctga tcgaacgcgc gccgggaccg
     1801 aagtactcga cgaccgcgcc cgtgccgccc ttgacggtga ggatcccggc gaccttcaag
     1861 atgacgtcct tgggtgccgc ccagccggac agctcgccgg tgagatgcac gccgatcagc
     1921 ttcgggacgc gcgtgttgaa gggccaaccc gccatcacgt cgaccgcgtc ggcaccgccg
     1981 acaccgatcg cgaccatgcc gagaccgccg gcgttcggcg tgtgcgagtc ggtgccgatc
     2041 atcatgccgc cggggaacgc gtagttctcg agcacgacct ggtggatgat gccgctgccc
     2101 ggcttccaga acccgatgcc gtacttcgcc gacaccgtgc gcaggaactc gtagacctct
     2161 tggttggtgt cgagcgcgac ctgcatgtcg gcgtccgcgc cgagccgggc ttggatcagg
     2221 tggtcgcagt gcaccgtcgt gggcaccgcg gcgcgcgaca gcttggccag catgaactgc
     2281 aacagcgcca tctgcgccgt tgcgtcctgc atggcgaccc ggtcggggcg gtagtcggcg
     2341 tagtcgacgc cgcgtgcgag accgaccgcg cgcgggtcgt cggcgtgtgc gatgaggacc
     2401 ttctcggcga aggtcaacgg gcggccgagc cgctcgcgtg cgatgtcgac gcgctcggga
     2461 tagcgcgcgt acaccgattc gacgaagtcg acgggcgtac cgggggtgac ggactcaggc
     2521 atgctgcaac actcctgcgc tcaacgagaa gtcgatctcg aacgtggtgc gaccggcggg
     2581 ataccggtgc tcggaataga ggacgggcgc gtcgtcgacg tcgaaggtcg tccggcgcag
     2641 cagcaacagg ggcgaaccgg cgtcgacgtc gagcaggcgc gcatggtcgg gcgcggccag
     2701 ctcggcggtg atcgtctgct gcgcgtgcgc aagtgagacg ccgcgcacgg gcaacaagtc
     2761 gtagaacggc gcgcgctcga cgtgggcgcg cgacaacccg gcgccgagat cgccgcggac
     2821 ccacaccgtc acgagcgcga acggttcgtc gtcggcgagg ttcacgcgtt cgacgcgcag
     2881 cacgtcggca tcgtcacgta atcccagcgc gccggccacc gcggtcggtg cggcaacgaa
     2941 ggcgaacgag aggatgcggc ggctcggccg ggcaccggcc gcttcgaccg cggcttcgac
     3001 ggtcgtgacc cggcccaacg gctgacgcac cggatcgagg gccgcgaacc aacccgatcc
     3061 gcgccggctc acgacgagac cctcgcggcg cagctggtcg agggcccggc gcaccgtgac
     3121 ccggctggcg ccgtaggccc ggctcagctc ggcctcactg gccagggcgc ccccgggccc
     3181 gacgtcgccc gcggcgacac gcgcccggat cgcatcggcc agctcgaggt accgcacgac
     3241 ttgtattcta cttgtctacc gacgagaaga caagctggtg gacgtcagtc gccccagtag
     3301 cgctgctccc gccagggatc gccgtagttg tggtagccgt tgcgctccca gaaaccgggt
     3361 tcgtcgtggt cgaggaactc gaagccgcgc agccacttcg ccgacttc
//
LOCUS       DASUGA010000845         3272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1076220, whole genome shotgun sequence.
ACCESSION   DASUGA010000845 DASUGA010000000
VERSION     DASUGA010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076220"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VFX21_16680"
     CDS             complement(<1..518)
                     /locus_tag="VFX21_16680"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5097662.1"
                     /translation="MPLVGGEPAKRVSNWPMWVLGLVIMVDQIDQNIVRGVVDQLKTD
                     FGINDAQIGLLLSSFVLVNGLVTVPAGYLADRWHRTRTIGHTVVGWSLITMLTAAATS
                     FGGLLVLRSLLGFGQAITEPSAASLLSDYYPTSERGRVFSVQQVLVFIGIGGGIAIGG
                     AVGQAFGWQAAFL"
     gene            609..806
                     /locus_tag="VFX21_16685"
     CDS             609..806
                     /locus_tag="VFX21_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097663.1"
                     /translation="MPRHRPDARDLPERDVEGIEQLPEDEQEAVAEAAAENPDDTTRR
                     EAIEQELMELGRSDAGADLGD"
     gene            834..1256
                     /locus_tag="VFX21_16690"
     CDS             834..1256
                     /locus_tag="VFX21_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097664.1"
                     /translation="MAVRIAIAIAAFAVLAGVAWWLERRRRTDAPTQSSTALPAQLDR
                     HDFVRPDAPWLVALFTSANCDSCAGLIDKAAPLESPDVAVTELEFPAARALHDRYHIA
                     AAPTTLVADADGVVRASIVGAFNATDLWNALAELRDAG"
     gene            complement(1253..>3272)
                     /gene="ftsH"
                     /locus_tag="VFX21_16695"
     CDS             complement(1253..>3272)
                     /gene="ftsH"
                     /locus_tag="VFX21_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX5097665.1"
                     /translation="AEPPTAAEQRMSPAPEEQWRPPERTPRNGQGQGTKPPARPRWMP
                     YVIIGLVVAIFLVWQAAPSTTPARADLTYGKFMSLVEDKKVDEIKYDSSSGRINGKFV
                     ENFAEDGKNEFSTQGPQNGLPDPDINKLNENNVNINYKARSTDWISTLLVWVLPLALL
                     VGLWWFIARRAQGQMGAVMNIGRSRAKVYATDKPKTTFGDVAGYGAVKEEITEVVDFL
                     KQPGKFKEIGARIPKGVLLVGPPGTGKTLIARAVAGEAGVPFISITGSDFMEMFVGVG
                     AARVRDLFQTARKQAPAIIFVDEIDSIGRKRGAGLGGGHDEREQTLNQMLSEMDGFEA
                     TEGIVMMAATNRPDILDPALLRPGRFDRQIMVPLPTQDERVEILKVHMRDKKMDPDVD
                     VNVVARGTPGMSGADLANLVNEAALFAVRRGEHSVHRQDFDAARDRVLMGLKRESLAL
                     SGEEKEIVAYHEGGHAVCAYVLEHADPLHKVTILPTGMALGVTHQLPVEERHIYKREY
                     IADSLVVRLGGRVAEELVFGHQSTGAQNDLVGNTELARKMVREWGMSDRVGPMAWGSQ
                     GAVFLGEDLMHSRDYSDETARVIDEEVERILRDDEDRCRKVLTEYRAGLDDVAQALLE
                     RETLEGTEVGDIVDAAMGRKVGGPRVIVHADGTEEEATTPLLPTEPPL"
BASE COUNT          520 a         1081 c         1104 g          567 t
ORIGIN      
        1 aggaacgcgg cttgccagcc gaacgcttgc ccgaccgcgc cgccgattgc gatgccgccg
       61 ccgataccga tgaacaccaa cacctgctgc accgagaaga cgcggccgcg ttcgctcgtg
      121 gggtagtagt cggacagcaa actcgcggcc gacggctcgg tgatggcttg cccgaagccg
      181 agcagcgacc gcagcacgag cagaccgccg aagctcgtcg ccgcggcggt cagcatggtg
      241 atcagactcc aaccgacgac cgtgtgaccg atagtgcgcg tgcgatgcca ccggtcggcg
      301 aggtaaccgg cgggcaccgt cacgaggccg ttcaccaaca cgaacgacga cagcagcagg
      361 ccgatctggg cgtcgttgat gccgaagtcg gtcttcagct ggtcgacgac gccgcgcacg
      421 atgttctggt cgatctggtc gaccatgatg acgaggccga gcacccacat cggccagttc
      481 gagactcgtt tcgccggttc gccccccacg agcggcattt tcgcatacga cccgcgcggg
      541 tagaccaact cgcgggcggc tccgcgtgcc aggacgaatg tccaggagga atgtccagga
      601 ggaatgtgat gcctcgtcat cgacccgacg cccgcgatct tcccgagcgc gacgtcgaag
      661 ggatcgagca actgcccgaa gacgagcagg aagcagtggc cgaagcggcg gccgagaatc
      721 ccgacgacac cacgcgccgc gaggccatcg aacaagagct gatggagctg ggtcgttccg
      781 acgccggtgc cgatctcggc gactagctcg gatggcagta cgttcagacg ttgatggctg
      841 tgcgtattgc gattgcgatc gccgcgttcg cggttctcgc cggcgtggcg tggtggctcg
      901 aacggcgccg gcgcactgac gcaccgaccc agtcgagcac cgcgttgccg gcgcaactcg
      961 atcgtcacga tttcgtgcgc ccggacgcac cgtggctcgt cgcgctgttt acgtccgcca
     1021 actgcgactc gtgcgcgggg ctcatcgaca aagcagcgcc cctggagtca cccgacgtcg
     1081 cggtgaccga actcgagttt cccgcggcgc gtgcgctgca cgatcgctac cacatcgcgg
     1141 ccgcaccgac gacgctcgtc gccgatgccg acggtgttgt gcgcgcgtcg atcgtcggtg
     1201 cgttcaacgc aaccgatttg tggaacgcgc tcgctgagtt acgcgacgcc ggctagagcg
     1261 ggggctcggt cgggaggagg ggcgtggtcg cctcttcctc cgtaccgtcg gcgtgaacaa
     1321 tcacgcgcgg cccgccgacc ttgcggccca tcgcggcgtc gacgatgtca ccgacctcgg
     1381 tgccttcgag cgtctcccgt tcgagcagtg cctgggcgac gtcgtcgagg ccggcgcggt
     1441 actcggtgag cacctttcgg caccggtctt cgtcgtcgcg caggatccgc tcgacctctt
     1501 cgtcgatgac gcgggcggtc tcgtcggagt agtcgcgcga gtgcatgagg tcctcgccga
     1561 ggaacaccgc accctgggag ccccacgcca tggggccgac gcggtcggac atgccccact
     1621 cgcgcaccat cttgcgggcg agttcggtgt tgccgacgag gtcgttctgc gcaccggtcg
     1681 actggtgccc gaagacgagt tcttcggcga cgcgaccacc gaggcggacc accaacgagt
     1741 cggcgatgta ttcgcgcttg tagatgtggc gctcctcgac cggcagctgg tgcgtgacac
     1801 cgagcgccat gccggtcggc aggatcgtca ccttgtgcag cggatcagcg tgctcgagga
     1861 cgtacgcgca caccgcgtgc ccgccctcgt ggtacgcgac gatctccttc tcctcaccgg
     1921 agagggcgag cgactcgcgc ttgaggccca tgagaacgcg gtcgcgtgcc gcatcgaagt
     1981 cctggcggtg caccgagtgt tcgccgcggc gaaccgcgaa cagcgcggct tcgttcacga
     2041 ggttggcgag gtcggcacca ctcatgccgg gcgtgccccg agcaacgacg ttgacgtcga
     2101 cgtcggggtc catcttcttg tcgcgcatgt ggaccttgag gatctccacg cgctcgtcct
     2161 gtgtcggcag cggcaccatg atctggcggt cgaaacgacc cggacgcagc aacgcagggt
     2221 cgaggatgtc gggccggttc gtggcggcca tcatcacgat gccttcggtc gcctcgaagc
     2281 cgtccatctc ggagagcatc tggttgagcg tctgctcgcg ctcgtcgtgc ccgccgccga
     2341 ggccggcacc gcgcttgcgg ccgatggagt cgatttcgtc gacgaagatg atcgcgggcg
     2401 cctgcttgcg cgcggtctgg aagaggtcgc gcacgcgcgc cgcgccgaca ccgacgaaca
     2461 tctccatgaa gtcggaaccg gtgatcgaga tgaagggcac cccggcctcg ccggcgaccg
     2521 ctcgcgcgat gagcgtcttg ccggtgccgg gcggaccgac gagcaacacg cccttgggaa
     2581 tgcgcgcgcc gatctccttg aacttgccgg gctgtttgag gaagtcgacc acctcggtga
     2641 tctcttcctt gaccgcgccg tagccggcga catcgccgaa ggtggtcttc ggtttgtcgg
     2701 tcgcgtagac cttggcgcgc gaccggccga tgttcatgac cgcgcccatc tggccctggg
     2761 cccggcgcgc gatgaaccac cacaagccga cgagcaacgc gagcggcaac acccagacga
     2821 gcagcgtgct gatccagtcg gtggaacgcg ccttgtagtt gatgttgacg ttgttctcgt
     2881 tcagcttgtt gatgtccggg tcgggcagac cgttctgcgg accctgcgtc gagaactcgt
     2941 tcttgccgtc ctcggcgaag ttctcgacga acttgccgtt gatacggccg ctcgacgagt
     3001 cgtacttgat ctcgtcgacc ttcttgtctt cgacgagcga catgaacttg ccgtaggtga
     3061 ggtcggcgcg cgccggcgtt gtgctgggcg cggcctgcca caccaagaag atggcgacga
     3121 cgagcccgat gatgacgtac ggcatccagc gcgggcgcgc cgggggcttc gttccctggc
     3181 cctgtccgtt acgcggggtg cgttccggcg gtcgccattg ttcttcgggg gctgggctca
     3241 tgcgttgctc cgcagcggta ggcggctctg ca
//
LOCUS       DASUGA010000846         2528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_798586, whole genome shotgun sequence.
ACCESSION   DASUGA010000846 DASUGA010000000
VERSION     DASUGA010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798586"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFX21_16700"
     CDS             <1..201
                     /locus_tag="VFX21_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019886158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5097666.1"
                     /translation="NRAAPLTDRECEVLRLVATGKTNREIASELAISEHTVARHLQNI
                     FLKLDLPSRAAATAYAYEHDLV"
     gene            296..715
                     /locus_tag="VFX21_16705"
     CDS             296..715
                     /locus_tag="VFX21_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097667.1"
                     /translation="MTTTTDTSTIERLSREFNRCFETLDAPEEVFAADAFFDLYPPLW
                     RFQVQGREPFAAQLRAIAEGEVRARVLRVEPTASGFVLEHEETQRVPGTGDVVARRIW
                     LCTVRDGRITDVVGYCNGGWDDALRARHAAEAPMLRP"
     gene            730..1890
                     /locus_tag="VFX21_16710"
     CDS             730..1890
                     /locus_tag="VFX21_16710"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5097668.1"
                     /translation="MTTTQRTPATTEAVAERVRALAPSIAARAAEIEAARRLPLDLLD
                     DLKRAGCFRLLLPTSHGGIGAGLPDAMRVLEILARADGSTGWTVMIGAGSWCDLTGLP
                     RATFDALYGGDRDVVTAGAFNPSGSIEPVDGGYRVRGRWAFASGCEHADVIYGNCVES
                     IVDGHPQLRAAVFSPDEVTLEDTWNVSGLRGTGSHHFHADVVVPAERTFVPLGGEPCI
                     DDPIIHFPIVSLIGFAVACVATGIAQGALHDIVELATAKVPLLAPGPLAANPRFQYEL
                     ASADTELRAARAYLYDSAARAWDIACTGGEFTLEDRARLRAASVWATTRAAAVVDFAY
                     GAGGGTSLYAESPLQRRQRDVHAVTQHFIVKPDTLTTAGAILAGQDVDVMVF"
     gene            1924..>2528
                     /locus_tag="VFX21_16715"
     CDS             1924..>2528
                     /locus_tag="VFX21_16715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5097669.1"
                     /translation="MSEERPTKVALVTGASRGIGKAIAVELARAGYDVAILARTVTEG
                     EAREHSSTVTRSDTSPLPGSLDATADLVRAEQRRCLPVPADLLDHVSLVHAVGVVLAT
                     WGRIDVLVNNGRYVGPGHMDRILDTPIPVLRDHLEANALAPVVLVKEIVPGMIERGGG
                     TVINITSSAGYEDPPAPAGAGGWGLGYAFSKGAIHRIAGVLA"
BASE COUNT          383 a          887 c          867 g          391 t
ORIGIN      
        1 aaccgggccg cgccccttac cgaccgtgag tgcgaggtcc tcagactggt ggcgaccggg
       61 aagacgaacc gcgagatcgc gtccgagctc gccatcagcg aacacacggt cgcccggcat
      121 ttgcagaaca tcttcttgaa gctggatctg ccgtcgcgcg ccgccgcgac cgcgtacgcc
      181 tacgagcacg atctcgtctg accggcccgc gtggtcagaa ttcaccaccc gtgcccggcc
      241 ccgaatggtg catcccgtcg atgcggcgcg gccggtcgtt ccgtaccttc gtcgcatgac
      301 gacgacgaca gacaccagca ccattgaacg actgagtcgc gagttcaacc gttgcttcga
      361 aacgctcgac gcgcccgagg aagtgttcgc ggccgacgcg ttcttcgacc tgtacccgcc
      421 gctgtggcgg ttccaggtgc aagggcgcga gccgttcgcc gcacagttgc gggcgatcgc
      481 cgaaggcgag gtgcgcgcac gcgtcctgcg cgtcgaaccg acagccagtg ggttcgtgct
      541 ggagcacgag gagacacaac gggtccccgg caccggcgac gtcgtcgcgc gacgcatctg
      601 gttgtgcacg gtccgcgacg gacgcatcac cgacgtcgtc ggctactgca acgggggttg
      661 ggacgacgcg ctgcgggcgc gccacgcggc cgaagccccg atgctgcggc cgtgacggga
      721 ggagcgatca tgacgacgac tcagcgcact ccggccacga ccgaggcggt ggccgaacgc
      781 gtgcgggcgc tcgcgccgtc gatcgcggcg cgcgccgccg agatcgaagc cgcccggcgg
      841 ctcccactcg acctgctcga cgacctgaag cgggccggct gcttccggct gctcctgccc
      901 accagccacg gcggcatcgg tgcgggcctg cccgatgcca tgcgcgtcct ggagatactg
      961 gcccgagccg acgggtccac cggctggacc gtgatgatcg gtgccggatc gtggtgcgat
     1021 ctcaccggtc tgccgcgcgc cacgttcgac gcgctgtacg gcggcgatcg ggacgtcgtc
     1081 accgccggtg cgttcaatcc gagcgggtcg atcgagccgg tcgacggcgg ctaccgcgtg
     1141 cgggggcggt gggcgttcgc gagcgggtgt gagcacgcgg acgtcatcta cggcaactgc
     1201 gtggagtcca tcgtcgacgg ccacccgcaa cttcgcgccg cagtcttctc tcctgacgaa
     1261 gtgacgctcg aagacacatg gaatgtctcc gggctgcgcg gcaccggcag ccaccacttt
     1321 cacgccgacg tcgtggtccc cgccgaacgc accttcgtgc cactcggggg cgaaccgtgc
     1381 atcgacgacc cgatcattca cttccccatc gtctcgttga tcggcttcgc agttgcgtgc
     1441 gtcgcgacgg gcatcgcaca aggtgcactc cacgacatcg tcgagctcgc cacggccaaa
     1501 gtgcccctgc tcgcgccggg accgcttgcg gcgaatccgc gcttccagta cgagctcgcg
     1561 tcggcggaca ccgagctgcg cgcggctcgg gcgtacctct acgacagcgc ggcgcgggcg
     1621 tgggacattg cgtgcacagg cggcgagttc acactcgagg accgcgcccg cttgcgggcc
     1681 gcctcggtct gggccacgac gcgcgccgcg gccgtcgtcg acttcgccta cggcgcgggc
     1741 ggcgggacgt cgttgtacgc cgagtcgcca ctccagcggc gccagcgcga cgtccacgcc
     1801 gtcacgcagc acttcatcgt gaagccggac accctgacga cggccggcgc catcctcgcc
     1861 ggtcaggacg tggacgtgat ggtgttctga ttatggttcg gcgcacccag acggaggtgc
     1921 gtcgtgtccg aggaacgccc cacgaaggtc gcgttggtca ccggtgcgag tcgcgggatc
     1981 ggcaaggcca tcgccgtcga gctggcgcgc gcgggctatg acgtcgcgat cctcgctcgc
     2041 accgtcaccg aaggtgaagc gcgtgagcac agctcgacgg tcacgcgatc cgacacgtca
     2101 ccgcttccgg ggtcactcga tgcgacggcg gatctcgtgc gcgccgagca acgtcggtgc
     2161 ctaccggtgc cggccgatct cctcgatcac gtgtcgctcg tccacgcggt tggcgtcgtg
     2221 ctcgcgacct ggggccgcat cgacgtactc gtgaacaacg gtcgctacgt cgggcccggc
     2281 cacatggacc gtatcctcga cacgccgatt ccggtgctgc gcgatcacct cgaggccaac
     2341 gcactcgcgc cggtcgtgct cgtcaaggaa atcgtgcccg gcatgatcga gcgcggtggt
     2401 gggaccgtga tcaacatcac gtcgtcggcc gggtacgaag acccgccggc gccggccggt
     2461 gcgggcggat gggggctcgg ctacgcgttc agcaagggtg cgatccatcg gatcgccggc
     2521 gtgttggc
//
LOCUS       DASUGA010000847         2120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2049546, whole genome shotgun sequence.
ACCESSION   DASUGA010000847 DASUGA010000000
VERSION     DASUGA010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2049546"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="VFX21_16720"
     CDS             <1..1037
                     /locus_tag="VFX21_16720"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5097670.1"
                     /translation="WTARVPERFRDRAPRIERFDQGDAWVIEGVADPINFGMNACAGL
                     APEAMHAWTRFEDMRRGGYDPMARLAEMDTDGVDAEVLYPTPRLMQGIVANTDAELHV
                     ELVRGYNDWLSEYVAAAPDRFGGLCLLPNRGAQHAVAEIERVWGRPGIRGALIGCYPN
                     GTLEPDDADDPVWARLVEARAPLHIHVSLSQTMPAAHRVPLPGYGRFFDAPNRIVQLI
                     FAGVFDRFPALDLVVAEVDCGWVPYFKEQIDNNYSRLAPLNDFRIADVPSAYVERHVH
                     FTYMTDGFGIANRDAIGVERVLWSSDYPHISSDWPHSRAVVAATFADVPEAERDAILA
                     GNAQRLYGFG"
     gene            complement(1034..1837)
                     /locus_tag="VFX21_16725"
     CDS             complement(1034..1837)
                     /locus_tag="VFX21_16725"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5097671.1"
                     /translation="MQHALYVPPFACFGDVHFLVDFACVAEEAGWDGVFLWDHILYED
                     DVPFVDAWIAMAAIAAATSRLRIGPLITPLPRRRPQVVAREAVTLDHLSHGRLTLGVG
                     LGIDFWREFGAFGEAAEDDAARARLLDDGIEILRGLLSGEEFSYAGETASVDRVRFLP
                     KPVQQPSIPVWSAVTWLPAREGPVRRAARLDGVMPFNPSGPMTPDIIRELRDRIAAVR
                     GNDSFDICSHGPREQVAEFEAAGVTWFCDSLYWESRDDAMADARTGPPR"
     gene            complement(1848..>2120)
                     /locus_tag="VFX21_16730"
     CDS             complement(1848..>2120)
                     /locus_tag="VFX21_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX5097672.1"
                     /translation="GVTRELRACQDLGARGLLSFEANVLPRLTADAWSDDDAYRRLVQ
                     LNAALARAGNPRSLKAVLDVGPLRDPYLPLTSAEADRLEEAVRALR"
BASE COUNT          332 a          777 c          704 g          307 t
ORIGIN      
        1 tgtggacggc ccgggtgccc gagcgcttcc gggaccgcgc gccgcgcatc gagcggttcg
       61 accagggtga cgcatgggtg atcgaaggcg tcgccgaccc gatcaacttc ggcatgaacg
      121 cgtgcgcggg cctcgcaccc gaagccatgc acgcgtggac gcgcttcgag gacatgcgcc
      181 gcggtggcta cgacccgatg gcgcgtctcg ccgagatgga caccgacggc gtcgacgccg
      241 aggtgctcta ccccaccccg cgcctgatgc agggcatcgt cgccaacacc gatgcggagc
      301 tccatgtcga gctcgtgcgc ggctacaacg actggctctc cgagtatgtc gcggccgcgc
      361 ccgaccggtt cggcggtctg tgcctgctcc cgaaccgcgg cgcgcagcac gcggtcgccg
      421 agatcgaacg cgtgtggggc cggcccggca ttcgcggcgc gctcatcggt tgctacccga
      481 atggcacgct cgaacccgat gacgccgacg atcccgtgtg ggcgcgcctc gtggaggccc
      541 gcgcaccgtt gcacatccat gtgagcctgt cgcagacgat gccggccgcg caccgcgtcc
      601 cgctgcccgg ttacggccgc ttcttcgacg ccccgaaccg catcgtgcag ctgatcttcg
      661 ccggcgtctt cgatcgcttc cccgcgctcg acctcgtcgt cgccgaggtc gactgcggct
      721 gggtgccgta cttcaaggag cagatcgaca acaactacag tcgtctggcg ccgctgaacg
      781 acttccgcat cgccgacgtg cccagtgcgt acgtcgagcg gcacgtgcac ttcacgtaca
      841 tgaccgacgg cttcggcatc gcgaaccgcg atgcgatcgg cgtcgagcgg gtgctctggt
      901 cgagcgacta tccgcacatc tcgagtgact ggccgcactc gcgcgccgtc gtcgccgcga
      961 ccttcgccga tgtgcccgaa gccgaacgcg acgcgatcct cgccggcaac gcgcagcgct
     1021 tgtacgggtt cggctagcga ggcggtcccg ttcgcgcgtc cgccatcgcg tcgtcgcgcg
     1081 actcccagta aagcgagtca cagaaccacg tgacaccggc ggcctcgaac tcggccactt
     1141 gttcacgcgg gccgtgcgag cagatgtcga acgagtcgtt cccgcgcacc gcggcaatac
     1201 gatcgcgcag ctcgcggatg atgtcgggtg tcatcggccc gctcgggttg aacggcatca
     1261 caccgtcgag ccgcgccgcc cgtcgtaccg gcccctcgcg cgcgggcagc cacgtcaccg
     1321 ccgaccagac cgggatgctc ggttgctgta ccggcttcgg caggaaccgc acccgatcga
     1381 ccgacgcggt ctccccggcg tacgagaact cctcgcccga cagcaggccg cgcaggatct
     1441 cgatgccgtc gtccaacagc cgggcccggg ccgcgtcgtc ttcggccgct tcgccgaacg
     1501 caccgaactc gcgccagaag tcgatcccga gcccgacgcc gagggtcaac cggccgtgac
     1561 tgaggtggtc gagggtgacc gcctcccggg ccaccacctg cggccggcgg cgcggcagcg
     1621 gtgtgatcag cggaccgatg cgaaggcgcg aggtcgcggc cgcgatcgcc gccatcgcga
     1681 tccacgcgtc gacgaacggc acatcgtcct cgtagaggat gtggtcccag aggaacacgc
     1741 cgtcccaccc ggcctcctcg gccacacacg cgaagtcgac gaggaagtgg acgtcgccga
     1801 agcaggcgaa cggtggcacg tagagcgcat gctgcatgcg cgcgacccta acgaagggct
     1861 cggacagcct cctcgagccg gtcggcctcg gccgacgtca gcggcaggta cgggtcccgc
     1921 aacggaccca catccagcac tgccttgagc gagcgcgggt tgccggcgcg cgccagtgcc
     1981 gcgttcagtt gcacgagccg gcggtacgcg tcgtcgtcgc tccaggcatc ggccgtcaac
     2041 cgtggcaaga cgttcgcttc gaagctcaac aggccgcggg cgccgaggtc ttggcaggcc
     2101 cgcagctcac gcgtcacacc
//
LOCUS       DASUGA010000848         2144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1771754, whole genome shotgun sequence.
ACCESSION   DASUGA010000848 DASUGA010000000
VERSION     DASUGA010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771754"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1674)
                     /locus_tag="VFX21_16735"
     CDS             complement(43..1674)
                     /locus_tag="VFX21_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013830351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5097673.1"
                     /translation="MDFTPREVDDEHARRYVREGFWVDQSMGELLGTGLSDGAANAFV
                     TRSDRHAFRGTIADVDALARRVATGLRARGIGPGDPVAFQLPNWLEAAATFYAVSYLG
                     AVIVPIVHFYGPKEVGYILRKTRVKALITTQYFGHQNFANNLAQLREEGLPDLEWVAF
                     VDTGEGIAGGDIDFADLAAADALVTPASVDPREPALIAYTSGTTSDPKGVVHSHRTIN
                     AEIAQLSAMMANRENPAMIVGAPVGHGIGMLAALLTPVFRRSAIHLIDVWDPGTVLAA
                     MLAENLSSGQGSTFFLTSLLDHPDFDPDRHAKLMPFVGLGGSAVPAAVGERCFKLGIE
                     VARSFGSTEHPSITGCRPEEPREKRINTDGRPLPGVELRLVDDDGKDVDAGTPGEIWS
                     RGPDCFVGYTDTAATRNAFSADGWYMTGDIGVLDEDGFLAITDRKKDIIIRGGENVSA
                     QEVEELLVRMPDVAEVAVVAAPDARLGEVACVFFRMQPGRPAPTLAEMRTHLDNAGLA
                     KQKWPEHVRSVDEFPRTPSGKVQKFVLRERLRAEM"
     gene            1712..2074
                     /locus_tag="VFX21_16740"
     CDS             1712..2074
                     /locus_tag="VFX21_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004003991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5097674.1"
                     /translation="MTLRLWTVVIDCNDPARLAHWWAEAIGWKIFYESDDEVVITVPD
                     ERWPGFVFGRVPEGKEIKNRLHLDFVPDDQDAEVTRLLELGATRADVGQTGDEGWVVL
                     ADPEGNEFCVLRSRHGDG"
BASE COUNT          365 a          709 c          721 g          349 t
ORIGIN      
        1 gccggattga gtctgcgggg agtgacaaat ggcccgccgg gctcacatct cggcccggag
       61 ccgctcgcgg agcacgaact tctgcacttt gccgctgggc gtccgcggga attcgtcgac
      121 cgatcgcacg tgctcgggcc acttctgctt ggcgagaccg gcgttgtcga gatgggtgcg
      181 catctcggcg agcgtcggcg cgggccggcc gggctgcatg cggaagaaca cgcaggcgac
      241 ttcgccgagg cgcgcgtcgg gcgcggcgac caccgccact tcggcgacgt cgggcatccg
      301 cacgaggagc tcttcgacct cttgcgcgct gacgttttca ccaccgcgaa tgatgatgtc
      361 cttcttgcgg tcggtgatcg cgaggaaacc gtcttcgtcg agcacgccga tgtcgccggt
      421 catgtaccag ccgtcggcgc tgaacgcgtt cctggtcgca gccgtgtcgg tgtagccgac
      481 gaagcagtcg ggcccgcggc tccagatctc gccgggcgtg cccgcgtcca cgtccttgcc
      541 gtcgtcgtcg acgagccgga gctcgacacc cggcaacggg cgaccgtcgg tgttgatgcg
      601 cttctcgcgc ggttcctcgg gccgacaacc ggtgatcgac ggatgctcag tcgaaccgaa
      661 gctgcgcgcg acctcgatgc cgagtttgaa gcagcgctcc cccaccgcgg cgggcacagc
      721 cgaacccccg agcccgacga acggcatgag cttggcgtgg cggtcgggat cgaaatcggg
      781 atgatcaagc aggctcgtga ggaagaacgt cgagccctgg ccggaactca agttctcggc
      841 gagcatcgcc gcgagcaccg tgcccggatc ccacacgtcg atgaggtgga tcgccgagcg
      901 gcggaacacg ggtgtcagca gcgccgcgag catgccgatg ccgtgcccga ccggcgcgcc
      961 gacgatcatc gccggattct cgcgattcgc catcatcgcg ctgagctgcg cgatctcggc
     1021 gttgatcgtg cggtgcgagt gcacgacacc tttcggatcg gaggtcgtac ccgacgtgta
     1081 cgcgatgagc gcgggctcac gcgggtcgac cgatgcgggc gtcaccaacg cgtccgccgc
     1141 cgccaggtcg gcaaagtcga tgtcgccgcc cgcaatgcct tcaccggtgt cgacaaacgc
     1201 gacccattca agatccggca agccttcctc gcgcagctgc gcgaggttgt tggcgaagtt
     1261 ctgatggccg aagtactgcg tcgtgatcaa cgccttcacg cgcgtcttgc gcaggatgta
     1321 gccgacttcc ttcggaccgt agaagtgcac gatcggcacg atgaccgcgc ccagatacga
     1381 gaccgcgtag aacgttgcgg cggcttcgag ccagttcggc agttgaaacg caaccggatc
     1441 gcccggacca atgcctcgcg cgcgcaagcc ggtcgcgacc cggcgcgcga gcgcgtcgac
     1501 gtcggcgatc gtgccccgga aggcgtggcg gtccgagcgc gtcacgaacg cattggcagc
     1561 accgtcggac aatccggtcc cgagcagctc acccatgctc tggtcaaccc agaatccttc
     1621 ccgcacgtag cggcgggcgt gttcgtcgtc aacttcgcgc ggcgtgaagt ccatcggttg
     1681 cagttgtagt ggaacgggta cgttgcgcgc catgacactg cgcttgtgga ccgtcgtgat
     1741 cgactgcaat gacccggcgc gcctcgcaca ctggtgggcg gaggcgatcg gctggaagat
     1801 cttctacgag agcgacgacg aggtcgtcat caccgtcccc gacgaacggt ggcccggatt
     1861 cgtgttcgga cgcgtccccg aaggcaagga gatcaagaac cgtctccacc tcgatttcgt
     1921 gcccgacgac caggacgccg aagtgacccg tctcctcgaa ctgggagcga cgcgcgccga
     1981 cgtcggtcaa actggcgacg agggctgggt cgtgctcgcc gatcccgaag gcaacgagtt
     2041 ctgcgtcctg aggtcgcgcc acggcgacgg ctgacgccgg ccgacgccag gtgcggccga
     2101 aaccaagaga ggatcaacgc atgtctcagc tgctccgagt acag
//
LOCUS       DASUGA010000849         2346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_244471, whole genome shotgun sequence.
ACCESSION   DASUGA010000849 DASUGA010000000
VERSION     DASUGA010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244471"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VFX21_16745"
     CDS             <1..605
                     /locus_tag="VFX21_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX5097675.1"
                     /translation="VGRADQARKQVAQGVDIVIAQGTEAGGHTGEIATMVLVPEVVEA
                     VHPTPVLAAGGIGSGKQMAAAMALGAAGVWTGSIWLGVAEADTAPESQNRLFAAEARD
                     AVRSRSLTGKPARMLRTDWTAAWERDDCPGTLPMPLQFMATQKARARIARSERRDLFG
                     MPVGQIVGSMHNVRTTKQVVLDMVEEYIETVEKMSRGISE"
     gene            589..1464
                     /locus_tag="VFX21_16750"
     CDS             589..1464
                     /locus_tag="VFX21_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007731949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097676.1"
                     /translation="MASRSNAERARRSWRALEPVHGMIYFAPEALEQYSKLGITHHRM
                     GYFASRSAPMGAVPAEVVIATFFNFYPLLVHKAIPAAWDVTTPAAMIEARFAAADQAL
                     RRAFGDEVGSADMARLAELARSVALVACETPEGRPLFAGHVTVEWPDDPHLVVWHAQT
                     LLREFRGDGHVAVLTTAGLTAVEALVVHGATGQVPDVMLQGSRAWPEDLWAAGVESVS
                     ARGWLAADGTLTQAGRAHREQVEADTDTLAARAYARLSEDDAETMVQLSRRFSRMVVD
                     GGLLAPDSLTAARAD"
     gene            1471..>2346
                     /locus_tag="VFX21_16755"
     CDS             1471..>2346
                     /locus_tag="VFX21_16755"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5097677.1"
                     /translation="MLKLHWFLPTGGDARDVLPDADDSHRRPPALPYLAQVATACDAL
                     GFDGMLTPCGTGCEDAWLATASLLPLTRRVKFLVAFRPALLSPTLAAQMASTYQRLSD
                     GRLLVNIVTGAEPAELARFGVFEDKETRYERTGEFVEIMRGAWSGQPFDYAGAHYRVE
                     AATTRDVPNPVPRIYFGGASDAAERIAARHVDVYLAWGEPPAMVSERLERMRSLATAE
                     GRSLDFGIRFHVIARPTSEEAWAAAGALRARMSDESIAAAQADFATTQSVGQQRMAAL
                     HGGDDDSLLVYPNVWA"
BASE COUNT          335 a          779 c          880 g          352 t
ORIGIN      
        1 tggtgggccg ggccgaccag gcccgcaagc aggtcgcgca gggtgtcgac atcgtgatcg
       61 cgcagggcac cgaggccggc ggccacaccg gcgagatcgc gacgatggtg ctggtaccgg
      121 aagtcgtcga ggccgtgcac ccgacgccgg tgctggccgc gggtggcatc ggttccggca
      181 agcagatggc cgccgcgatg gcgctcggtg cggccggcgt gtggacggga tcgatctggc
      241 tcggcgtcgc ggaggccgac accgcgccgg agagccagaa ccggttgttc gcggcggagg
      301 cgcgtgacgc ggtgcgctcg cgttcgctca cgggcaagcc cgcgcgcatg ctgcgtaccg
      361 actggacggc cgcgtgggag cgcgacgact gcccgggcac gctgccgatg ccgttgcagt
      421 tcatggcgac gcagaaggcg cgcgcccgca tcgcgcggtc ggaacgccgt gacctcttcg
      481 ggatgcccgt cggccagatc gtcggcagca tgcacaacgt gcgcacgacg aagcaggtcg
      541 tgctcgacat ggtcgaggag tacatcgaga ccgtcgagaa gatgtcgcgt ggcatctcgg
      601 agtaacgccg agcgggcgcg ccgttcgtgg cgcgcgctcg aaccggtgca cgggatgatc
      661 tacttcgcac cggaagcgct cgagcagtac tccaagctcg gcatcacgca tcaccggatg
      721 ggctacttcg cgtcgcgctc cgcgcccatg ggcgcggtgc cggccgaagt cgtgatcgcg
      781 acgttcttca acttctatcc gctgctcgtg cacaaggcga tcccggccgc atgggacgtc
      841 acgacgcccg cggcgatgat cgaagcgcgc tttgcggcgg ccgaccaggc gttgcggcgc
      901 gcgttcggcg acgaggtcgg ttccgcggac atggcgcggt tggccgagct ggcgcgctcg
      961 gtcgcgttgg ttgcgtgcga gacgcccgag ggtcgcccgc tgttcgccgg gcacgtgacg
     1021 gtggagtggc cggacgatcc gcatttggtc gtgtggcacg cgcagacgct gttgcgcgag
     1081 ttccgcggtg acgggcacgt cgcagtgttg acgaccgcgg gtctgaccgc ggtcgaagcg
     1141 ctcgtcgtac acggcgcgac cggccaggta cccgacgtga tgttgcaggg gtcgcgggcg
     1201 tggcccgagg acttgtgggc ggcgggtgtc gagtcggtat cggcgcgcgg gtggctcgca
     1261 gcggacggca cgctgacaca agcgggccgc gcgcatcgcg agcaagtcga agccgacacc
     1321 gacacgctcg ccgcgcgcgc gtacgcgcgt ttgtcagaag acgacgcgga gaccatggtg
     1381 cagctctccc ggcgcttcag ccgcatggtc gtcgacggcg gcctgctcgc ccccgactcc
     1441 ctcaccgccg cgcgcgcgga ctgacgcccg gtgctgaagc tccattggtt ccttccaacg
     1501 ggtggtgacg cacgcgacgt cctccccgac gccgacgatt cgcatcgccg cccgcccgcg
     1561 ttgccgtatc tcgcgcaggt ggcgaccgcc tgcgacgcgc tcggcttcga cgggatgctc
     1621 acgccgtgcg gcacgggctg cgaggacgcg tggctcgcga cggcgtcgtt gctcccgttg
     1681 acgagacggg tgaagttcct cgtcgcgttc cggcccgcgc tgttgtcacc cacgctggcc
     1741 gcgcagatgg cgtcgacgta ccaacggctg tccgacggtc gcttgctcgt gaacatcgtg
     1801 acgggggcgg aaccggcgga actggcccgc ttcggcgtgt tcgaggacaa ggagacgcgc
     1861 tacgagcgca ccggcgagtt cgtcgagatc atgcggggcg cctggagcgg ccagccgttc
     1921 gactacgcag gcgcgcacta ccgggtcgaa gcggcgacga cgcgcgacgt gccgaacccg
     1981 gtgccgcgca tctacttcgg tggcgcgtcc gacgcggcgg aacggatcgc ggcccggcac
     2041 gtcgacgtgt atctcgcgtg gggcgagccg ccggcgatgg tgagcgagcg attggagcga
     2101 atgcgttcac tggcgacagc cgaaggtcgc tcgctcgact tcggcatccg gttccacgtg
     2161 atcgcccggc cgacttccga ggaagcgtgg gcggcggcgg gtgcgctccg ggcgcgcatg
     2221 agtgacgagt cgatcgcggc cgcgcaggcc gacttcgcca cgacgcaatc cgtcgggcag
     2281 caacgcatgg ccgcactgca cggcggtgac gacgacagtc tcctcgtgta cccgaacgtg
     2341 tgggcc
//
LOCUS       DASUGA010000850        10039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_799200, whole genome shotgun sequence.
ACCESSION   DASUGA010000850 DASUGA010000000
VERSION     DASUGA010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 10039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10039
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799200"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1057)
                     /locus_tag="VFX21_16760"
     CDS             complement(152..1057)
                     /locus_tag="VFX21_16760"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX5097678.1"
                     /translation="MFDLWTLAERQYGVVTREQARDQGYSKHQIDTMLKAGRLIPVHR
                     AVYRVAGSPRTARQRAMAACFWAGPGSLCSHQTAGALLKLEGVRSRDLHITALQTVRR
                     KTERPLVFHRTIELEPLDCAVVDDLPATSATRAILDLGAVISGEQLEVAFESARRLGL
                     TSANAVARRFERFGGRGREGSSRVRQLLEAVGERPAESTLEVKANRLLRRSRLRQPQL
                     QVWITGGDGRRYRVDFVWPAARLIVECDGFAWHGQHLAWKRDRRRVAALEAAGWRVVH
                     VTWDDVVKHPAETLFRISTALEQAA"
     gene            complement(1132..2532)
                     /locus_tag="VFX21_16765"
     CDS             complement(1132..2532)
                     /locus_tag="VFX21_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HEX5097679.1"
                     /translation="MSRAATLGELRSSGWTSRPVKHEIRANAIARLRAGEPIVDGVVG
                     YEDTVIPQLVNALLAGHDVIFLGERGQAKTRIIRSLVGLLDEYMPIVAGSEINDDPFH
                     PISTHARVLVAERGDETPIEWVHRERRYGEKLATPDTSIADLIGEVDPIKVAEGRYLS
                     DELTIHYGLVPRTNRGIFSINELPDLAERIQVGLLNVLEERDVQIRGYKIRLPLDVIL
                     FASANPEDYTNRGRIITPLKDRFGSQIRTHYPLETELEVEIIEQEAAPLAAEGLSVSV
                     PPFMTELVAEMSQLARRSPHVNQRSGVSARLSITNYETLIANATRRALINGENDVVPR
                     VSDLDALAASTQGKIEIETLDDGRESQVLERILKAATLEVFRARVRPEKLGPIVASFD
                     EGTIVHTGEDVPSGDYRDLMSKLDGIGAVLSDLEVGESPAGIASGIEFVLEGLHLTKR
                     LNKDAVGARATYRARG"
     gene            2619..4727
                     /locus_tag="VFX21_16770"
     CDS             2619..4727
                     /locus_tag="VFX21_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097680.1"
                     /translation="MVRSVDLGGITLAGTWRARESDSELAKTFPDPAFDDSGWSTVEL
                     PHHWRSVAALAESDGPVLYRRQWSAGPPARGRRRFLVADGIFYYGDLWLDGGYVGATE
                     GYFVPHAFEVTEALAARDEHVVALEVACPPQRDRTAKRTVTGIFGHWDAADTTRNPGG
                     PWRPLRVLETGPVRLGTVRVGCREATEENSRLVCDLMLDAVDGPLDAQLHARVVDDAG
                     RVLLESTRDVVLAAGENRQEWVLTVEQAPRWWPRTLGEQPMCDVTIEVEVAGATSDQR
                     VVRTAFREVHCDGFRFSINGETMFLKGTNLAPARMHLADATPDEIRRDLELACDANLD
                     FVRMHAHVAREELYDAADELGLLVWQDLPLQWGYARGVRKQAVRQGRAMVDLLGHHPS
                     VFLWCGHNAPFAMDREPGETMGRGAIAKLAATTVLPTWSKQVLDRSIARGLSRRDNSR
                     PVVKHSGVFPGIADSGTDAHLYFGWYLGDIGGLARAMRQWPRMARFVSEFGAQAVPTT
                     AAWMQPELWPDLDWERLAREHMMQVEVFERRMPPRESKTFDEWRELTQAYQAALLQLQ
                     VEDLRRIKYAPTGGFAQFSFADPHPMVSWSVLDHERVPKRGYAALRDACRPVLPMVEP
                     RQGLVHVVNDTRQEYADAIVEVSVDGRIHRFAGDIPADAVAYVGRVDLADAVDVEVTL
                     THHAVDRVVNRYPLLVLRAS"
     gene            complement(4714..5817)
                     /locus_tag="VFX21_16775"
     CDS             complement(4714..5817)
                     /locus_tag="VFX21_16775"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5097681.1"
                     /translation="MRAIEVLEQGIADGTQIGAQLAVSRGGVPVVDVALGEARAGVPM
                     TTDTMMIWFSMTKAVTSVAVAQQWERGALDVDDPVAKYIPEFAANGKADTTLRHLLTH
                     TAGLPWADGILESWPWRESRAENLARIYAATPDYPAGTRAGYHAGAGMSVLGAVVELV
                     SGTRFDEYVRAGIFEPLEMHDCWIGMPPDRYDAYGDRVGIMHATNVSPPTPVKGVDAR
                     RQTSVPVPGGNGRGPMRELVHLYEMLVAGGARNGVRLLTPQTVSAISARHRVGMVDET
                     FGVVLDWGLGFAIDTYITGSHSSPRAFGHGGHLSSAAICDPEHGLAMAVVCNGMPGGA
                     KHHARMDAIATAVYEDLGITAPTREKVYPVNSL"
     gene            5889..6716
                     /locus_tag="VFX21_16780"
     CDS             5889..6716
                     /locus_tag="VFX21_16780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HEX5097682.1"
                     /translation="MRTAFAGEVMATTTTPPPRAGTEPIRRTKPTGPPIIRFYRSAIG
                     KKWVMAITGLVLMSFVLVHLIGNLKVYLSKDELNLYGEALRDMPGHLLPRTVLLWTIR
                     IGLILAFVFHIHAAYSLTVMNRRARPQTYKSKRDYIAADWASRTMRWTGVIVLLYVLF
                     HLADLTWGSANPEFVRGDPYNNLIYSMQRPVVAGIYILATIALGFHLLHGAWSLFQSL
                     GINNPKYNGARRQFAIAFMAIIVIGNISFPIAVQAHLIEPACPHTDPVADCSEKSGL"
     gene            6713..8629
                     /locus_tag="VFX21_16785"
     CDS             6713..8629
                     /locus_tag="VFX21_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEX5097683.1"
                     /translation="MTLDSRIPDGPLAEKWEQHKFDIKLVNPSNKRRFDIIVVGTGLA
                     GASAAASLGELGYNVKVITFHDSPRRAHSIAAQGGINAAKNYKNDGDSVQRLFYDTIK
                     GGDYRSREANVYRLAELSVNIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYARG
                     QTGQQLLLGAYQALMRQVHMGTVKLYPRTEMLDLVVKDGAARGIVCRDLESGEVFSMS
                     GHAVVLATGGYGNVFYLSTNAKNSNVTAAWRASKRGAYMANPCFTQIHPTCIPASDEF
                     QSKLTLMSESLRNDGRVWVPKQMDDPRSPDQIPEDERDYFLERRYPAFGNLVPRDVAS
                     RAIKREVDAGRGVGSLKNGVYLDFSESIGRLSRDVVEERYGNLFEMYERITGEDPYVV
                     PMRIYPAIHYTMGGLWVDYNLMSNLPGLFVMGEANFSDHGANRLGASALMQGLADGYF
                     VLPYTIGNYLAPLLGEKPLAVDDPEFVAAEKQVDDQVRKLLSIKGNRTVDDIHRELGH
                     VLWDNCGMARSEASLTKALTEIPAIRDEFWKNVRVLGEDDERNQALEKAGRVADFLEF
                     GELLVRDALHRNESCGGHFREEYQSEEGEAQRDDEHFAYVAAWEWTGLGNEPVLHKEP
                     LVFENVHLSTRSYK"
     gene            8656..9423
                     /locus_tag="VFX21_16790"
     CDS             8656..9423
                     /locus_tag="VFX21_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEX5097684.1"
                     /translation="MKITLTAWRQKGPNTEGGFETYEVPGVSPDMSFLEMLDTVNERL
                     MLEGKEPIAFDHDCREGICGSCGMMINGQAHGPERGTATCQLHMRKFRDGDHITIEPW
                     RAAAFPIVKDLIVDRSAFDRIIESGGYISVNTGGTPDGNDIPIPKPVADAAMDAAACI
                     GCGACVAACPNGAAQLFTAAKMQHLNLMPQGQAERWDRAVNMVDTMESYFGSCTNHRE
                     CEAACPKEISIDFIALMNKDYVKAQLKQRRLVGQTFP"
     gene            complement(9433..>10039)
                     /locus_tag="VFX21_16795"
     CDS             complement(9433..>10039)
                     /locus_tag="VFX21_16795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX5097685.1"
                     /translation="AAPAGYANGVTVVDELRDEAARCTACDLYKDATQVVFGEGPPDA
                     DLMLMGEVPGDKEDLAGHTFVGPAGHVLDDALGEIGLDRTQIYLTNAVKHFKFEQRGK
                     VRIHKTPNRTEQIACSRWWQAELDAVHPKVIGLLGAVAGQAVLGPKYRVTKQRGEWIQ
                     LKNGVWALGTVHPSSILRAQERRAEEYAAFVRDLQKLTERV"
BASE COUNT         1623 a         3406 c         3374 g         1636 t
ORIGIN      
        1 cttcgcgtgg ctccgagaac gcgaagcggc cggcatccag ctcgtgttct cccacgacac
       61 cgaccaatgg aactccctcg gcgagttcat ctgacgcgtt cgttgtcaat ccagcccgct
      121 gagagcgggc cggattgaca acgaacaacg atcacgccgc ttgttcgagt gccgttgaga
      181 tgcggaacaa cgtctcggcg ggatgcttga cgacgtcgtc ccatgtcacg tgcacgacgc
      241 gccagcccgc cgcctccagc gcggcgactc gccggcggtc gcgcttccac gccaagtgct
      301 gcccgtgcca cgcgaagccg tcgcactcga cgatgaggcg cgcagcgggc caaacgaagt
      361 cgacccgata cctccggccg tcgccacccg tgatccagac ctgcagttgc ggctgccgga
      421 gccggctcct gcggagcaac cggttcgcct tcacttcgag cgtggattcg gccggccgtt
      481 ccccgacggc ttcgagcagt tgccgcacgc gggagctgcc ctcacgaccg cgcccgccaa
      541 atcgctcgaa ccgacgcgct accgcattcg cgctcgtcag cccgagccgg cgcgcactct
      601 cgaacgcgac ctcgagttgt tcgccgctga tgactgcacc gagatcgagg atggcgcgcg
      661 tcgccgacgt cgccggcaag tcgtcgacga cggcgcagtc caacggctcg agttcgattg
      721 tgcggtggaa cacgagcggc cgttcggtct tgcgccgaac tgtctgaagc gcagtgatgt
      781 gcaagtcgcg agacctgacc ccttcgagct tgaggagcgc gcccgccgtt tgatgggaac
      841 acaacgaacc aggcccggcc cagaagcacg cggccatggc gcgttgtcga gccgtccgcg
      901 gtgaacctgc cacgcggtag accgcgcgat ggaccgggat caggcgtccc gccttcagca
      961 tcgtgtcgat ctggtgcttg gagtagccct ggtcgcgtgc ctgctcgcgc gtcacgactc
     1021 cgtactggcg ctcggcgagc gtccataggt cgaacattgg aacctccgcc tccgaatatc
     1081 gggctggatt gccagagaac gggggaagag ggtggaggaa gtgcgcgtgt actagccgcg
     1141 ggcgcggtag gtcgcccttg cgccgacggc gtccttgttg aggcgcttgg tgaggtgcag
     1201 gccttcgagc acgaactcga tgccggacgc gatgccggcc ggtgattcgc cgacttcgag
     1261 gtcggagagc accgcaccga tgccgtcgag cttcgacatc aggtcgcgat agtcgcccga
     1321 cgggacgtcc tcgccggtgt ggacgatcgt gccctcgtcg aacgacgcga caattgggcc
     1381 cagcttctcg ggccgcacgc gggcccggaa cacctcgagc gtcgcggcct tgaggatgcg
     1441 ctccagcact tgcgactcgc ggccgtcgtc gagcgtctcg atctcgatct tgccctgcgt
     1501 cgacgccgcg agcgcgtcga ggtcggagac gcgcggcacg acgtcgttct cgccgttgat
     1561 cagcgcgcgc cgcgtcgcgt tcgcgatcag cgtctcgtag ttcgtgatcg agagccgcgc
     1621 cgagacaccg gagcgctggt tcacgtgcgg cgaccgccgc gcgagctgcg acatctccgc
     1681 gacgagctcg gtcatgaacg gcggcacgct gacggagagg ccttcggccg cgagcggcgc
     1741 cgcctcctgt tcgatgatct cgacttcgag ctcggtctcg agcgggtagt gcgtgcggat
     1801 ctgcgaaccg aagcggtcct tcagcggtgt gatgatgcgg ccgcgattcg tgtagtcctc
     1861 ggggttcgcg gaggcgaaga ggatgacgtc gagcggcagg cggatcttgt agccgcggat
     1921 ctgcacgtcg cgctcctcga gcacgttcag cagaccgacc tggatgcgct cggcgaggtc
     1981 gggcagctcg ttgatcgaga agatgccgcg gttcgtgcgg ggcacgaggc cgtagtggat
     2041 cgtcagctcg tcggagagat agcggccctc ggcgaccttg atcgggtcga cctcgccgat
     2101 gaggtccgcg atcgacgtgt cgggcgttgc gagcttctcg ccgtagcggc gctcacggtg
     2161 cacccactcg atcggcgtct cgtcgccgcg ttcggcaacg agcacacgcg cgtgcgtcga
     2221 gatcggatgg aacggatcgt cgttgatctc gctgcccgcg acgatcggca tgtactcgtc
     2281 gagcagaccg acgagagaac ggatgatgcg cgtcttcgcc tggccgcgtt caccgaggaa
     2341 gatgacgtcg tggcccgcga gcagcgcgtt caccaactgc gggatgacgg tgtcctcgta
     2401 accgacgaca ccgtcgacaa tcggttcgcc ggcgcggagc cgcgcgatcg cgttggcgcg
     2461 gatctcgtgc ttgacgggcc gcgacgtcca gccgctgctc cggagttcgc cgagtgtcgc
     2521 tgctctgctc atatgcggag tgcggacgca gcggagatct tctcgccgga ggccgatcga
     2581 tgcggagcgg agggagccat ggtcacgagc ataggtctat ggttcggagc gtggatctcg
     2641 gcggcatcac cctcgccggg acgtggcgcg cgcgcgaatc cgacagcgaa ctggccaaga
     2701 cgttcccgga ccctgcgttc gacgacagtg gatggtcgac cgtcgagctc ccgcaccatt
     2761 ggcgttcggt tgcggcgttg gcggagtccg acggcccggt cttgtatcgc cggcagtggt
     2821 ccgccggacc gcccgcgcgc ggccgtcgcc gctttctcgt tgccgacggc atcttctact
     2881 acggcgacct gtggctcgac ggtgggtacg taggcgcgac cgagggctat ttcgtcccgc
     2941 atgcgttcga agtgaccgag gcgctcgccg cacgcgacga gcacgttgtc gcgctcgagg
     3001 tcgcgtgccc accccagcgc gaccgcactg cgaagcgcac ggtcaccggc atcttcggcc
     3061 attgggacgc ggccgacacg acgcgcaacc ccggtggacc gtggcgtccg ttacgtgtgc
     3121 tcgaaaccgg tccggtccgc ctgggtacgg tgcgcgtcgg ctgccgcgag gcaaccgaag
     3181 agaacagccg gctggtgtgc gacctcatgc tcgacgcggt cgacggcccg ctcgacgcgc
     3241 agttgcacgc gcgcgtcgtc gacgacgccg gacgcgtcct gctcgagtcg acgcgcgacg
     3301 tcgtgctcgc cgccggcgag aaccggcagg agtgggtgct gacggtcgaa caggcgccgc
     3361 gatggtggcc ccgcacactc ggcgagcaac cgatgtgcga cgtcacgatc gaggtcgagg
     3421 ttgccggggc cacgagcgat cagcgtgtcg tgcgcaccgc gtttcgcgaa gtgcactgcg
     3481 acggcttccg gttctcgatc aacggcgaga cgatgttctt gaaggggacg aaccttgcgc
     3541 ccgcgcgcat gcacctcgcc gacgcgacgc ccgacgagat ccgccgcgac ctcgagctcg
     3601 cgtgcgacgc caacctcgac ttcgtgcgca tgcacgcgca cgtcgcccgg gaagagctgt
     3661 acgacgccgc cgacgagctc ggcctgctcg tgtggcagga cctcccgctc cagtggggct
     3721 acgcacgcgg tgtgcgcaag caggccgttc gccagggccg agccatggtc gatctgctcg
     3781 gccaccaccc gagcgtcttc ctctggtgcg ggcacaacgc gccgttcgcc atggaccgcg
     3841 agccgggcga gacgatgggt cgcggcgcga tcgcgaagct cgcggcgacg accgtcctgc
     3901 ccacgtggtc gaagcaggtg ctcgaccgct cgatcgcacg cgggttgtcc cggcgcgaca
     3961 acagccggcc cgtcgtgaag cacagcggcg tgttccccgg catcgccgac agcggtaccg
     4021 acgcgcacct ctacttcggg tggtatctcg gcgacatcgg cggtctcgcc cgcgcgatgc
     4081 ggcagtggcc gcgcatggcg cgcttcgtgt cggagttcgg cgcgcaggcc gtaccgacga
     4141 ccgcggcatg gatgcaaccg gagctgtggc ccgatctcga ctgggagcgg ctcgcgcgcg
     4201 agcacatgat gcaggtcgag gtgttcgagc ggcgcatgcc gccgcgggag agcaagacgt
     4261 tcgacgagtg gcgcgagctc acgcaggcgt accaagccgc gctcctgcaa ctacaggtcg
     4321 aagatctccg gcgcatcaag tacgcgccga ccggtggctt cgctcagttc tcgttcgccg
     4381 acccgcaccc gatggtgagc tggtcggtcc tcgaccacga gcgcgtcccc aaacgcgggt
     4441 acgccgcgct gcgcgacgcg tgccggccgg tgctcccgat ggtcgaaccc cggcaaggcc
     4501 tcgtacacgt cgtcaacgac acgcgccagg agtatgccga cgcgatcgtc gaggtctcgg
     4561 tcgacgggcg catccaccgg ttcgcgggcg acatcccggc cgacgcggtc gcgtatgtcg
     4621 gccgcgtaga cctcgccgac gcggtcgacg tcgaggtcac gctgacccac cacgccgtcg
     4681 accgcgtggt caaccgctac cccttgttgg tgctcagagc gagttgaccg gatacacctt
     4741 ctcgcgcgtc ggtgcggtga tgccgaggtc ttcgtagacg gcggtcgcga tggcgtccat
     4801 acgcgcatgg tgcttggcgc caccgggcat gccgttgcac acgaccgcca tcgcgaggcc
     4861 gtgctccgga tcgcagatcg cggccgatga caggtgcccg ccgtgcccga acgcacgcgg
     4921 cgacgagtgg ctgccggtga tgtacgtgtc gatcgcgaag ccgagtcccc agtcgagcac
     4981 cacgccgaac gtctcgtcga ccatgccgac gcgatgacgg gcggagatcg cgctcaccgt
     5041 ctgcggtgtg agcagccgca caccgttgcg cgcgccgccg gcgaccaaca tctcgtacag
     5101 gtgcaccaac tcgcgcatcg gtccgcggcc gttgccaccg gggaccggca ccgacgtctg
     5161 acggcgcgca tcgacgccct tgaccggcgt cggcggcgag acgttggtcg catgcatgat
     5221 gccgacgcgg tcgccgtacg cgtcgtagcg gtcgggtggc atcccgatcc agcagtcgtg
     5281 catctccaac ggctcgaaga tcccggcgcg cacatactcg tcgaagcgcg tgccggacac
     5341 gagctccacg accgcgccca acaccgacat gcccgcaccc gcgtggtagc ccgcgcgcgt
     5401 accggcggga tagtcgggcg tggccgcgta gatccgcgcg aggttctcgg cgcgcgattc
     5461 acgccacggc cacgactcga ggatgccgtc ggcccacgga agcccggcgg tgtgcgtcaa
     5521 cagatggcgc agcgtcgtgt cggccttgcc gttggccgcg aactcgggga tgtacttcgc
     5581 gacgggatcg tcgacgtcga gcgcgccccg ttcccactgc tgcgcgaccg cgacggaggt
     5641 cacggccttt gtcatcgaga accaaatcat catcgtgtcg gtcgtcatcg gcacgccggc
     5701 gcgggcctcg ccgagcgcga cgtcgacgac cggcactccg cctcgcgaca ccgcgagctg
     5761 ggccccgatc tgggtgccgt cggcgatgcc ctgttcgagg acctcgatgg ctcgcatctg
     5821 cttctccgat cgattcggca attccccgcg gtgtgagcgt agattcgttt cacaacgtca
     5881 cgagccccgt gcgaaccgca tttgcggggg aggtcatggc caccacgacg acgccgccgc
     5941 cccgggccgg caccgaaccg atccggcgga cgaagcccac gggcccgccg atcatccgct
     6001 tctaccgatc ggcgatcggc aagaagtggg tcatggccat caccggtctg gtgctcatga
     6061 gcttcgtgct cgtgcacctc atcgggaacc tcaaggtcta cttgtcgaag gacgagctca
     6121 acctctacgg cgaggcgctg cgcgacatgc cgggccactt gctcccgcgc acggtgttgc
     6181 tgtggacgat tcgcatcggg ctgatcctcg cattcgtgtt ccacatccac gccgcgtact
     6241 cgctgacggt gatgaaccgg cgggcgcggc cgcagacgta caagtcgaag cgcgactaca
     6301 tcgccgccga ctgggcatcg cgcacgatgc gctggaccgg cgtcatcgtg ttgctctatg
     6361 tgctgttcca cctcgcggat ctcacctggg ggtcggccaa ccccgagttc gtgcgcggtg
     6421 atccgtacaa caacttgatc tacagcatgc agcgcccggt ggtcgcgggc atctacatcc
     6481 ttgcgacgat cgcgttgggc ttccacctgt tgcacggcgc ctggtcgttg ttccagagcc
     6541 tcgggatcaa caacccgaag tacaacggcg cgcggcgtca gttcgcgatc gcgttcatgg
     6601 cgatcatcgt gatcggcaac atctcgttcc cgatcgcggt gcaggcgcac ctcatcgaac
     6661 cggcgtgccc gcacaccgat cccgtcgccg attgctcgga gaagtcgggc ctgtgactct
     6721 tgattcgcgc attcccgacg gcccgctcgc cgagaagtgg gagcagcaca agttcgacat
     6781 caagctcgtc aacccgtcga acaagcgccg cttcgacatc atcgtcgtcg gcaccgggct
     6841 cgcgggtgcg tcggctgccg cctcgctggg cgagctcggg tacaacgtca aggtcatcac
     6901 gttccacgac tcgccgcgac gcgcgcactc gatcgccgcg caaggtggca tcaacgccgc
     6961 caagaactac aagaacgacg gcgacagcgt gcagcggctg ttctacgaca cgatcaaagg
     7021 tggcgactac cgatcgcgtg aagcgaacgt gtaccgcctc gccgagctgt cggtgaacat
     7081 catcgaccag tgcgtcgcgc agggcgtgcc gttcgcgcgt gagtacggcg gcctgctcga
     7141 caaccgttcg ttcggtggcg cgcaggtctc gcggacgttc tacgcgcgcg gtcagaccgg
     7201 ccagcagctg ttgctgggtg cgtaccaggc gttgatgcgc caggtgcaca tgggcacggt
     7261 gaagctgtat ccgcgcaccg agatgctcga cctcgtcgtg aaggacggcg ccgcgcgtgg
     7321 catcgtgtgc cgcgacctcg agtccggtga agtgttctcg atgtcgggtc acgcggtcgt
     7381 gctcgcgacg ggcggctacg gcaacgtctt ctacctgtcg acgaacgcga agaactcgaa
     7441 cgtgacggcg gcgtggcggg cgtcgaagcg cggggcctac atggcgaacc cgtgcttcac
     7501 gcagatccac ccaacgtgca tccccgcgag cgacgagttc cagtcgaagc tcacgctgat
     7561 gtcggagtcg ctgcgcaacg acgggcgggt gtgggtgccc aagcagatgg acgacccgcg
     7621 ctcccccgac cagatccccg aggacgagcg cgactacttc ctcgagcggc gctaccccgc
     7681 gttcggcaac ctggtgccgc gcgacgtcgc atcgcgcgcg atcaagcgcg aggtcgacgc
     7741 cggtcgcggt gtcgggtcgt tgaagaacgg cgtgtacctc gacttctcgg agtcgatcgg
     7801 ccggttgagt cgcgacgtcg tcgaggagcg ctacggcaac ctcttcgaga tgtacgaacg
     7861 catcaccggc gaagacccct acgtggtgcc catgcgcatc taccccgcga tccactacac
     7921 gatgggtggg ctgtgggtcg actacaacct gatgagcaac ctgcccgggt tgttcgtgat
     7981 gggcgaggcg aacttctccg accacggcgc gaaccgcctc ggcgcgtcgg cgctcatgca
     8041 gggtctcgcc gacggctact tcgtgttgcc ctatacgatc ggcaactacc tcgcgccact
     8101 cctcggcgag aagccactcg cggtcgacga ccccgagttc gtcgcggccg agaagcaggt
     8161 cgacgaccaa gtgcgcaagc tgttgtcgat caagggcaac cgcaccgtcg acgacatcca
     8221 ccgcgagctc ggtcacgtgc tctgggacaa ctgcggcatg gcgcgttcgg aggcgagcct
     8281 cacgaaggcg ctcaccgaga tccccgcgat ccgcgacgag ttctggaaga acgtgcgcgt
     8341 gctcggcgag gacgacgagc ggaaccaggc actcgagaag gccggccgcg tcgccgactt
     8401 cctcgagttc ggcgagctgc tcgtgcgcga cgcgctgcac cgcaacgaga gctgtggcgg
     8461 gcacttccgc gaggaatacc agtccgaaga aggcgaggcg cagcgcgacg acgagcactt
     8521 cgcgtacgtc gccgcgtggg agtggacggg cctcggcaac gagccggtcc tccacaagga
     8581 gcctctcgtc ttcgagaacg tccacctgtc gacccgttcc tacaagtagc ggagcctgac
     8641 gtgtccgatc gcacactgaa gatcacgctc actgcctggc ggcagaaggg cccgaacacc
     8701 gaaggcggtt tcgagacgta cgaggtgccg ggggtcagcc ccgacatgtc gttcctcgag
     8761 atgctcgaca cggtcaacga gcggttgatg ctcgagggca aggagcccat cgcgttcgac
     8821 cacgactgcc gcgagggcat ctgcggctcg tgcgggatga tgatcaacgg ccaggcgcac
     8881 ggcccggagc gcggcaccgc gacgtgccag ctgcacatgc gcaagttccg agacggcgac
     8941 cacatcacga tcgagccgtg gcgtgccgcc gcgttcccga tcgtgaagga cctcatcgtc
     9001 gaccgcagcg cgttcgaccg catcatcgag tcgggcggct acatcagtgt caacacgggt
     9061 ggcacacccg acggcaatga catcccgatc ccgaagcctg tcgcggacgc ggcgatggac
     9121 gccgccgcgt gcatcggctg cggtgcgtgc gtcgccgcgt gcccgaacgg cgcggcgcag
     9181 ctgttcaccg ccgcgaagat gcagcacctg aacctgatgc cgcagggcca agccgagcgc
     9241 tgggaccgcg cagtgaacat ggtcgacacc atggagtcgt acttcggcag ctgtacgaac
     9301 caccgcgagt gcgaagcggc ctgcccgaag gagatctcga tcgacttcat cgcgctgatg
     9361 aacaaggact acgtgaaggc gcagctcaag cagcgccgcc tcgtcggcca gacgttcccg
     9421 tagcgctccc ggtcacaccc gctcggtcag cttctgcagg tcgcggacga acgcggcgta
     9481 ctcctcggcg cggcgttcct gggcgcgcag gatcgacgat gggtggacgg tgccgagtgc
     9541 ccagacgccg ttcttcagct ggatccactc gccgcgctgc ttggtgacgc ggtacttcgg
     9601 tccgagcacc gcttgcccgg cgaccgcgcc gagcaggccg atcaccttgg gatgcaccgc
     9661 gtcgagctcc gcttgccacc accggctgca cgcgatctgt tcggtgcggt tcggtgtctt
     9721 gtggatccgc accttcccgc gttgttcgaa cttgaagtgc ttcaccgcat tggtgagata
     9781 gatctgcgtt cggtcgagac cgatctcgcc gagcgcgtcg tcgagcacgt ggcccgccgg
     9841 gccgacgaac gtgtggccgg cgaggtcctc tttgtcgccg ggcacctcgc ccatcaacat
     9901 caagtccgcg tcgggcggac cctcgccgaa cacgacctgc gtcgcgtcct tgtacaggtc
     9961 gcacgcggtg cagcgcgccg cttcgtcgcg cagctcgtcg acgacggtca caccgttcgc
    10021 gtaccccgct ggtgccgcc
//
LOCUS       DASUGA010000851         2454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_32782, whole genome shotgun sequence.
ACCESSION   DASUGA010000851 DASUGA010000000
VERSION     DASUGA010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32782"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="VFX21_16800"
     CDS             <1..1322
                     /locus_tag="VFX21_16800"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5097686.1"
                     /translation="TNAYSQHSVCAQSRISMFTGWYPHVAGHRTLEHLLAAHEPNVFA
                     RLRESGYHVALAGTRGDMFAAGGTRAVSDRFGFTVPPKLEDLGKWHASPFEPGSKWYD
                     AFYGGPVDGDLYEFDAATVRTAAEWLAERLPEPWCLFVALVFPHPPFTVERRWYDLYD
                     GVTMPAPVPTNFDGKPGFYREIHRRYGLDRMTVDDWAEITRTYYAMVTRVDDQLREVR
                     DALARSGQAENTVTFFFTDHGEYLGDYGLVEKWPSGVDDVLVRNPLIVHDPSGRAGAA
                     SSFVEMVDLTATLEDLAGLERGAHFGRTVRTLLADPTAPHRDAAFSEGGFLVQEEPLL
                     EAGDTGQYRHKQEIQHDRTELVGRAIAVRTEDWCYVERLYEGPELYDRRTDPLETTNL
                     AGRPEHAATQRDLRDRIFRWLFETSDVIPTRRDPRFDDDLRQALFGG"
     gene            1399..2004
                     /locus_tag="VFX21_16805"
     CDS             1399..2004
                     /locus_tag="VFX21_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013020936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX5097687.1"
                     /translation="MRKIKAALFISLDGVVEAPDQWHFPYFNEEMGEGVTEDMEGADA
                     LLLGRVTYDSFAGAWPGREAGGGEDAPFAKKLGDLRKVVVSRQRLTFTWRNSELLEGD
                     FVAAVTALKNEPGGDIGMSGSVSIVRQLLASGLLDELHLYVHPIAVRKGMRLFDDGAS
                     IPLQLVSSKTFETGVLSLVYAPAEDTRDAGYNDVTQYIAEK"
     gene            2011..2445
                     /locus_tag="VFX21_16810"
     CDS             2011..2445
                     /locus_tag="VFX21_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018471177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5097688.1"
                     /translation="MEQRISIVTIGVGNLARARAFYEALGWQGQEMQETVFIQAGGIA
                     VILWGRADLAEDAGLTDNGTDGFGGMALAHNVRSRDEVDAIMRSAHAAGATILKSARE
                     TFYGGYAGWFADPDGHVWEIAHNPGFTINADGTLTLPDFGAM"
BASE COUNT          414 a          808 c          842 g          390 t
ORIGIN      
        1 tcaccaacgc gtactcgcaa cacagcgtgt gcgcgcagag ccggatctcg atgttcaccg
       61 gctggtaccc gcacgtcgcg gggcaccgga cgttggagca tctcctcgcc gcgcacgaac
      121 cgaacgtgtt cgcgcgcctg cgcgagagcg gataccacgt cgcgctggcc ggcacgcggg
      181 gcgacatgtt cgcggccgga ggtacgcgcg cggtgagcga ccgcttcggc ttcacggtgc
      241 cgccgaagtt ggaggacctc gggaagtggc acgcgtcgcc cttcgaaccg ggctcgaagt
      301 ggtatgacgc gttctacggc gggcccgtcg acggcgatct ctacgagttc gatgccgcaa
      361 ccgtgcgcac cgcggccgaa tggctggccg aacgcctgcc cgaaccgtgg tgcctgttcg
      421 tcgcgctcgt gttcccgcat ccgccgttca cagtcgaacg acggtggtac gacctgtacg
      481 acggcgtcac gatgcccgcg ccggtgccga cgaacttcga cggcaagccc ggcttttacc
      541 gcgagatcca ccggcgctac gggctcgatc ggatgaccgt cgacgactgg gccgagatca
      601 cgcgcaccta ctacgcgatg gtgacgcgtg tcgacgacca gttgcgtgaa gtacgtgacg
      661 cgctcgcgcg ttccgggcag gcggagaaca cagtgacgtt cttcttcacc gaccacggcg
      721 agtacctcgg cgactacggc ttggtcgaga agtggccatc cggtgtcgac gatgtgctgg
      781 tgcgcaaccc gctgatcgtc cacgacccgt ccgggcgcgc gggcgcggca tcgtcgttcg
      841 tcgagatggt cgatctcacc gcgacgttgg aggaccttgc cggcctcgaa cggggcgcgc
      901 acttcgggcg cacagtgcgg acgctgctcg cggacccgac cgcgccgcat cgcgatgcgg
      961 cattcagcga aggcgggttc ctcgtgcagg aagagccgtt gttggaagcg ggtgacaccg
     1021 ggcagtaccg gcacaagcaa gagatccaac acgaccggac ggagctcgtc ggtcgcgcga
     1081 tcgcggtccg caccgaagac tggtgctacg tcgaacgcct ctacgagggt ccggagctct
     1141 acgaccgccg caccgatccg ctcgagacga cgaatctcgc gggccggccc gagcacgccg
     1201 cgacgcagcg cgacctgcgc gatcgcatct tccgctggtt gttcgagacg tccgacgtca
     1261 ttccgacgcg ccgcgaccct cgattcgacg acgacctgcg acaggccctc tttggtgggt
     1321 agtgcagggg agcgatgagt tcagtcgcgt cgtgcagtct caatggacac aacgtgcatt
     1381 cgtcactgga gcagcaccat gcgcaagatc aaggccgcac tgttcatctc cctcgacggt
     1441 gtcgtggaag cacccgacca gtggcacttc ccgtacttca acgaggagat gggcgagggt
     1501 gtcaccgagg acatggaagg cgccgacgcg ttgctcctcg gccgggtgac gtacgacagc
     1561 ttcgccggcg cctggcccgg ccgggaagcc ggcggtggtg aggacgcgcc gttcgccaag
     1621 aagctcggcg acctgcgcaa ggtcgtggtg tcgcgacagc ggctgacgtt cacgtggcgc
     1681 aactccgaac tgctcgaagg ggacttcgtc gccgcggtca ccgcgttgaa gaacgaaccc
     1741 ggcggtgaca tcgggatgag cggttccgtg tcgatcgtcc gtcagttgct ggcctcgggt
     1801 ttgctggacg aactgcacct gtacgtgcac ccgatcgcgg tgcgcaaggg catgaggctc
     1861 ttcgacgacg gcgcatcgat cccgttgcag ctggtgtcgt cgaagacgtt cgagaccggc
     1921 gtgctgtcgc tcgtctacgc gccggccgag gacactcgcg acgccgggta caacgacgtc
     1981 acccagtaca tcgcggagaa gtaggtcgtc gtggagcagc gcatcagcat cgtgacgatc
     2041 ggcgtcggca acttggcccg cgcccgcgcg ttctacgaag cgctcggctg gcagggacaa
     2101 gagatgcagg agacggtgtt catccaagcc ggcggcatcg ccgtcatcct gtgggggcgg
     2161 gccgacctcg cggaggatgc cgggctcacc gacaacggca ccgacggctt cggcgggatg
     2221 gcgctcgcgc acaacgtgcg gtcgcgcgac gaggtcgacg cgatcatgcg gagcgcacac
     2281 gcggcgggcg cgacgatcct gaagtcggcg cgcgagacct tctacggcgg ctacgcgggc
     2341 tggttcgccg accccgacgg ccacgtctgg gagatcgcgc acaaccccgg cttcaccatc
     2401 aacgccgacg gcaccctcac gctcccggac ttcggtgcca tgtagtcggt cggg
//
LOCUS       DASUGA010000852         2408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1216227, whole genome shotgun sequence.
ACCESSION   DASUGA010000852 DASUGA010000000
VERSION     DASUGA010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216227"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..1205
                     /locus_tag="VFX21_16815"
     CDS             45..1205
                     /locus_tag="VFX21_16815"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEX5097689.1"
                     /translation="MDKRKPQGLGTRSIHAGQEPDPTTGAVMTPIYATSTYVQKSPGV
                     HQGFEYTRSHNPTRFAYERCVADLEGGTRGYAFASGLAATSCVLETIDSGSHVICMDD
                     VYGGTFRLFERVRRRSAGLEFSFVDLTDEGAFEQAIRPNTRLVWIETPTNPLLKLVDV
                     ARIAERARKHGIKVAVDNTFATPILQRPLELGAHLVMHSATKYLNGHSDMVGGMVVVG
                     DDKELAEQMTFLQNAVGGVQGPFDSFLALRGLKTLHLRMHAHCANAGELASWLDTHPG
                     IEKVLYPGLKAHPHYELAKRQMSGFGGMLSVYVKGGLDGARRMMERCKLFAVAESLGG
                     VESLINHPAIMTHASVPPENRARLGIHDNLVRLSVGVEDLEDLRAELNDALS"
     gene            complement(1209..2276)
                     /locus_tag="VFX21_16820"
                     /pseudo
     CDS             complement(1209..2276)
                     /locus_tag="VFX21_16820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461341.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
BASE COUNT          394 a          829 c          826 g          359 t
ORIGIN      
        1 cttcggcaac gcctgacccg catacgcgaa ggaactgcaa caccatggac aagcgcaagc
       61 cccagggcct cggcacccgc tcgatccacg ccggccagga acccgacccg accacgggcg
      121 cggtgatgac gccgatctac gcgacctcga cctacgtgca gaagagcccg ggcgtgcacc
      181 agggcttcga gtacacgcgc tcgcacaacc cgacgcgctt cgcctacgag cgctgcgtcg
      241 ccgacctcga gggcggcaca cgcggctatg cgttcgcgtc cggcctggcc gccacgagct
      301 gcgtgctcga gacgatcgac agcggcagcc acgtgatctg catggatgac gtctacggcg
      361 gcaccttccg gctgttcgag cgcgtgcgca ggcgctccgc cgggctcgag ttcagcttcg
      421 tcgacctgac cgacgagggc gccttcgagc aggcgatccg cccgaacacg cgcctggtgt
      481 ggatcgagac gccgacgaat ccgctgctca agctggtcga cgtcgcgcgc atcgcggagc
      541 gggcgcgcaa gcacggcatc aaggtcgcgg tcgacaacac cttcgcgacg ccgatcctgc
      601 agcggccgct ggagctcggc gcgcacctcg tgatgcactc cgcgaccaag tacctcaacg
      661 gccactccga catggtcggc ggcatggtgg tggtgggcga cgacaaggag ctcgccgagc
      721 agatgacgtt cctgcagaac gcggtcggcg gcgtgcaggg accgttcgac agcttcctcg
      781 cgctgcgcgg tctgaagacg ctgcacctcc gcatgcacgc gcactgcgcg aacgcgggcg
      841 agctggcgtc gtggctcgac acgcatccgg gaatcgagaa ggtgctgtac cccgggttga
      901 aggcgcaccc acactacgag ctcgccaagc gccagatgtc cggcttcggc ggcatgctca
      961 gcgtgtacgt gaagggcgga ctcgacggcg cgcgccggat gatggagcgc tgcaagctgt
     1021 tcgccgtggc cgagtcgctc ggcggcgtcg agagcctgat caaccacccg gcgatcatga
     1081 cgcacgcctc ggtgccgccg gagaaccgcg cgcggctcgg catccacgac aacctcgtgc
     1141 ggctgtccgt cggcgtcgag gacctcgagg acctgcgcgc ggagctgaac gacgcgctgt
     1201 cgtaaccgtt acgactgcag gtcgggcagg tcgcggtaga ggtccagcgc ctgcgggttc
     1261 gccagcgcgt cggtgttctt caccggcctg ccgtggatca cctgccgcac cgcgagctcg
     1321 gtgatcttgc ccgagatcgt gcgcgggatg tcggcgacgt gcacgatctt cgccggcacg
     1381 tggcgcgggg tggtgttctc gcggatcgcg cgccggatgc ggtcgcgcag ggcgtcgtcg
     1441 agcgccacgc cctcgcgcag gcgcacgaac agcacgacgc gcacgtcgtt gtcccagtcc
     1501 tgcccgaccg cgatgctctc gagcacctcg ggcacgcgct cgacctggcg atagatctcg
     1561 gcggtgccga tgcgcacgcc gcccgggttc agcacggcgt cggaacgtcc caggatcacg
     1621 atgccgtcgt gctcggtgag cagggcgtag tcgccgtggc accacacgtt cggcacgcgc
     1681 tcgaaatacg cggcccggta cttggcgccg tccgcgtcgt tccagaagcc gacgggcatg
     1741 gacgggaacg gcgcgctgca cgagagctcg cccggctgct cgcgcaccgg cttgccgtcg
     1801 tcgtccagga tctcgacctt gagcccgagc ccgcggcact ggagctcgcc ctggtacacg
     1861 ggcagcagcg ggttgccgag cgcgaagcag gagatgatgt cggtgccgcc ggagatcgag
     1921 gccagcagca cgcgatgctt gatgtcgcgg tacacgtact cgaagctctc gtgcgagagc
     1981 ggcgagccgg tcgagagcac ggtcttgagg ttcagcagct tgtgcgtctc gatcggcctc
     2041 acgccggcct tctccgtcgc cgcgagccac ttggcgctgg tgccgaacac actgacgccg
     2101 accgcgtcgg cgagatccca cagcgcgttg ccgtcgggat gggacggcga gccgtcgtac
     2161 agcaccacgg tcgcgcccac ggccagcgac gacaccagcc agttccacat catccagccg
     2221 caggtggtgt agaagaagat cctgtcctcg cgcttgaggt cggtgtgcag caccagaagg
     2281 agcacgtcct ccacaccgac ctgacacgcg acgacaggat cttctactac accacctgcg
     2341 gctggatgat gtggaactgg ctggtgtccg cgctggcggt gggctccacg gtggtgctgt
     2401 acgacggg
//
LOCUS       DASUGA010000853         2537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2014601, whole genome shotgun sequence.
ACCESSION   DASUGA010000853 DASUGA010000000
VERSION     DASUGA010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2014601"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VFX21_16825"
     CDS             complement(<1..204)
                     /locus_tag="VFX21_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020301811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX5097690.1"
                     /translation="MHSDARREGAGSTHAYIPALDGVRAIAVVAVLLFHAGVPGATGG
                     YLGVSLFFTLSGFLMGSLLLRERH"
     gene            complement(346..1782)
                     /locus_tag="VFX21_16830"
     CDS             complement(346..1782)
                     /locus_tag="VFX21_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020786851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX5097691.1"
                     /translation="MTADLARLDASAQADLVRRGDMTPKELVEAAIARIESTNPALNA
                     VISTRFDRARDEASGDLADGPFRGVPFLLKDLFCPMEGEPAYDGTRLGREQDVRAPLD
                     SFLVRRFRAAGLVVVGRTNTPEFGLVPTTEPAVFGATRNPWDPARTAGGSSGGSAAAV
                     AAGLVPFAHASDGGGSIRIPAACCGLVGLKPSRGRVSAGPAGSELARFLSVQFAVTRS
                     VRDAAALLDVASGAEVGDTVVAPPPAAGTFVAAMAHPPRPLRIGLWSTLPGSSTPVSA
                     ECVRAAESTAGILEELGHHVEIAHPDALDDPARLGFFGAIWCVNAAAAVAHWSTIVGR
                     ELGPGDVEPLTWALATRGRAIPSPDYVLAVQGMQAWARRALAWWRDYDLLLTPTLGEL
                     PPRLGHFDEPDDPIAGYLRAGSFTPYTPVANQTGQPALSLPLGTAEHLPVGIHLVADN
                     GREDLLLAVAAQLEAAMPWTARTATVHA"
     gene            complement(1779..>2537)
                     /locus_tag="VFX21_16835"
     CDS             complement(1779..>2537)
                     /locus_tag="VFX21_16835"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5097692.1"
                     /translation="VDYQRACFQAYNRWIAEFCAVAPNRLLGIGQTAMRTPEEGIADL
                     EAIKALGLRGVMLPGIPPSADYDDPSYDEFWDAVVDLGIPPSFHILTSKTDTPYAPVR
                     GPRLNTFMSIIRGNQDIIGTLIFGAVFERHPDLRVVCVEADAGWAPHYMYRADHAYDR
                     HRNWLTAGELSKPPSAYFRENVYLTFQDDWVAFRTVDLMNVDRLMWANDFPHSDATWP
                     NSQALLAEHAVHLDEHQRARVLHDNCAELYGITA"
BASE COUNT          386 a          880 c          914 g          357 t
ORIGIN      
        1 gtggcgctcg cgcagcaaca gcgagcccat gaggaacccg gacagcgtga agaagaggct
       61 gacgccgagg taaccgccgg tcgcgccggg gacgccggca tggaacagca gcaccgcgac
      121 gacggcgatc gcccggacgc cgtccagcgc cgggatgtac gcgtgcgtgc tccccgcgcc
      181 ttcccgccgc gcgtcgctat gcatgcgacc ccggaccccc agccctgtca ccattggaga
      241 atggcgttcc ccggagtcca cggcggtatt ccgaccgccg taccccaccc ggactgaacg
      301 gcagccgggc gtagcccctg ccgtcagacc accgttcgta tgccgctaag catgcaccgt
      361 ggccgtgcgc gcggtccacg gcatcgccgc ttcgagctgt gcggccaccg cgagcaggag
      421 gtcttcccga ccgttgtcgg cgacgagatg gatgccgacg ggcaggtgct cggccgtgcc
      481 gagcggcagg gagagcgccg gttgcccggt ctggttcgcg accggtgtgt acggcgtgaa
      541 gctgccggcg cgcaggtagc ccgcgatcgg atcgtcgggt tcgtcgaagt ggccgagtcg
      601 cggtggcaac tcgccgagcg tcggcgtgag cagcaggtcg tagtcgcgcc accacgcgag
      661 cgcgcgtcgc gcccaggcct gcatgccctg taccgcgagc acgtagtcgg gcgacgggat
      721 cgcgcgaccg cgtgtcgcca gcgcccacgt gaggggttcg acgtcgccgg gaccgagctc
      781 gcggcccacg atggtgctcc agtgcgcgac cgcagccgcg gcgttcacac accagatcgc
      841 accgaagaac cccagtcgcg ccggatcgtc cagcgcgtcg ggatgcgcga tctcgacgtg
      901 atgcccgagc tcctcgagga tgcccgcggt ggactcggct gcgcgcacgc actcggcact
      961 cacgggtgtc gacgagccgg gaagcgtcga ccacagtccg atccgcagcg ggcgcggcgg
     1021 atgcgccatg gcggcgacga acgttcccgc cgcgggcggt ggcgcgacga cggtgtcgcc
     1081 tacttcggcg ccgctcgcga cgtcgagcag cgcggccgcg tcgcgcacgg agcgcgtcac
     1141 ggcgaactgc accgacaaga agcgcgccaa ctcactgccc gcgggaccgg ccgagacgcg
     1201 cccgcgcgat ggcttcagac caacgagacc gcaacacgcg gccgggatgc ggatcgaacc
     1261 gcccccgtcg gaggcgtgcg cgaacgggac gaggccggcc gcaaccgcgg ctgccgatcc
     1321 cccgctcgat ccgcccgcgg tgcgcgccgg atcccacggg ttgcgggtcg cgccgaacac
     1381 cgccggttcc gtcgtcggca cgagcccgaa ctcgggcgtg ttcgtgcggc cgacgacgac
     1441 gagccccgcg gcacggaagc gccgcacgag gaagctgtcg agtggcgccc gcacgtcttg
     1501 ttcgcgtccg aggcgcgtgc cgtcgtacgc cggctcgcct tccatcgggc agaagagatc
     1561 cttgagcaag aacggcacgc cgcggaacgg gccgtcggcg agatcgcccg acgcttcgtc
     1621 gcgcgcgcga tcgaatcgcg tcgagatgac cgcgttcaac gccgggttgg tcgattcgat
     1681 gcgcgcgatc gcggcttcga ccagctcctt cggcgtcatg tcgccccggc ggacgaggtc
     1741 ggcctgcgcg gacgcgtcga gccgggcgag gtcggccgtc atgcggtgat gccgtacagc
     1801 tcggcgcagt tgtcgtgcag cacgcgggcc cgctggtgct cgtcgaggtg caccgcgtgc
     1861 tcggccagca atgcctgcga gttcggccag gtcgcgtcgc tatgcgggaa gtcgttcgcc
     1921 cacatcagcc ggtcgacgtt catgagatcg acggtgcgga acgcgaccca gtcgtcttgg
     1981 aacgtgaggt agacgttctc gcggaagtac gcgctgggcg gcttcgacag ctcacccgcc
     2041 gtcaaccagt tgcggtgccg gtcgtaggcg tgatcggcgc ggtacatgta gtgcggcgcc
     2101 catccggcat cggcctcgac acagacgacg cgcagatcgg ggtggcgctc gaacaccgcg
     2161 ccgaagatca gcgtgccgat gatgtcctgg ttcccgcgga tgatgctcat gaacgtgttg
     2221 aggcgcggtc cgcgcaccgg cgcgtacggc gtgtcggtct tgctcgtgag gatgtggaac
     2281 gacggcggga tcccgagatc gacgaccgcg tcccagaact cgtcgtagct cgggtcgtcg
     2341 tagtcggcac tcggtggaat gccgggcagc atgacgccgc gcagaccgag cgccttgatc
     2401 gcctcgaggt ccgcgatccc ctcttccggc gtgcgcatcg cggtctggcc gatgccgagc
     2461 aaccggttgg gcgcaacggc gcagaactcg gcgatccacc ggttgtaggc ctggaagcac
     2521 gcgcgctggt agtcgac
//
LOCUS       DASUGA010000854         3539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_800497, whole genome shotgun sequence.
ACCESSION   DASUGA010000854 DASUGA010000000
VERSION     DASUGA010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800497"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..2800)
                     /locus_tag="VFX21_16840"
     CDS             complement(65..2800)
                     /locus_tag="VFX21_16840"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HEX5097693.1"
                     /translation="MTIRVGIVPHTHWDREWYSPFQTFRMRLVRLLDVLVPLLESDPS
                     YARFLLDGQTAILDDYLEVRPEAEAALRRLCASGRVHVGPWMVQMDEFMVSGETIVRD
                     LQMGFAQAAEYGGAMEIGYLPDLFGHIGQMPQILALAGIEHAVAWRGIPSTVDKTAFW
                     WQSPDGSRVRCEYLYGSYSNGRDIPSDAKGLVLRARDYQQELGDARIGDMLFMNGTDH
                     QMPQPWLGRVVAEANDQQDDFEFVVTSLPEYLPLQPSDDLPEVKGELRSGARANILMG
                     VASNRVDVHQACAAAERVLERRAEPLHALFTPAARYPSRLFELAWRSVVLNSAHDSSC
                     ACSHDEVVDQVLVRYYEARQIGDTCVRDAMHVLARDVDAPAGSTVVANPTARTRSGLV
                     EGTLPGDGPVHFVGPDGTPRPTQVIGTISGEGYSTMVTGQKVRWVLDLMRGTEFAGRQ
                     VASYDIVEDAGHGDDRFDDIVLQEAGPGETRIDLAELKASLLQRGSAGRSMRLRLLVA
                     PQRRLLFDTGPVDGFGWATFTPINAPAPAGPVVATEGALANDQIRVVVDLGDGTYAIE
                     TNDGLRVAALGRLVDGGDGGDTYNYSPPQTDRIVDRPDAVRVQTLEAGPVRARVLVES
                     DYTWPARALGDDKWCTARSDETEKVTVRTTLELRPAERFVRVTQEFDNRCRDHRLRAH
                     FPLPATVGFSDAECAFAVVRRGLTAEGGAHEFGLPTFPSRRFVDASDGRNGLALLHDG
                     LLEYEVVGDGTELALTLLRATGYLSRSEPSLRPNPAGPTDPVHGAQMLGEQKVEYAVL
                     PHRGDWRAADCYGAADAFTVPLERVRGGGTGGTRPPSGRALHVDGAEVSAVLRTPGGL
                     TVRVFRTEPTAGEVSIEHDGAPARGWNIDLRGRPVAPFEGTVTLKPWQIATLQIT"
     gene            2825..>3539
                     /locus_tag="VFX21_16845"
     CDS             2825..>3539
                     /locus_tag="VFX21_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018742735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5097694.1"
                     /translation="MAGSLDGKIALVAGATRAAGRGIAVQLGAAGATVYCTGRTTAAQ
                     RSEMNRPETIEETAALVDAAGGRGIAVQVDHLVPDQVRALVERIDAAHGALDILVNDI
                     WGVKIEWDKPVWESTLDNGLHTLRLGVDTHAITSHFAIPLMLKTPGGLVIEMTDGTNE
                     YNATNYRVSFFYDIAKAAVNRMAFALAHELAPNGRTAVALTPGWLRSEEMLDAYQVTE
                     ENWLDATKIQPHFAISESPA"
BASE COUNT          550 a         1243 c         1258 g          488 t
ORIGIN      
        1 cggagcggag ggcgggccaa caatccgggc tgcctcacgc gagcacgacc ggagcgacaa
       61 agagtcacgt gatttgtagc gtcgcgatct gccagggttt gagcgtcacg gtgccctcga
      121 agggggccac ggggcggcca cggaggtcga tgttccagcc tcgcgccggc gcgccgtcgt
      181 gctcgatgct cacttcgccg gccgttggct cggtgcggaa gacgcgtacg gtgaggccgc
      241 ccggggtgcg caggaccgcg gacacttcgg cgccgtcgac atgcaacgcg cggccgctcg
      301 gcggtcgggt gccgccggtg ccgccgccgc gcacgcgctc caacgggacg gtgaacgcgt
      361 cggccgcgcc gtagcagtcg gccgcgcgcc aatcgccgcg gtgggggagc accgcgtact
      421 cgaccttctg ctcgccgagc atctgcgcgc cgtgcacggg gtcggtcggg cccgcggggt
      481 tcggccgcag cgacggttcg ctgcgcgaca agtagccggt tgcgcgcagc aacgtgagcg
      541 cgagctcggt gccgtcgccg acgacctcgt actcgagcag gccgtcgtgc aacaacgcga
      601 gaccattgcg gccgtcggag gcgtcgacga agcggcgtga cgggaacgtc ggcagcccga
      661 actcgtgggc gccgccttcg gccgtcaggc cgcggcggac gaccgcgaac gcgcactcgg
      721 cgtcggagaa gccgacggtc gccggcagcg ggaagtgcgc gcgcagccga tggtcgcggc
      781 agcggttgtc gaactcctgc gtcacgcgca cgaatcgctc ggcgggccgg agctcgagcg
      841 tcgtacgcac ggtgaccttc tccgtctcat cgctgcgggc agtgcaccac ttgtcgtcgc
      901 cgagcgcccg ggccggccac gtgtagtcgg actcgacgag cacgcgcgcc cgcaccggtc
      961 ccgcttcgag cgtctgcacc cgcaccgcgt cgggccggtc gacgatgcga tccgtctgcg
     1021 gcggcgagta gttgtacgtg tcgccgccgt cgccgccgtc gacgaggcgc ccgagagcgg
     1081 cgacgcgcag cccgtcgttg gtctcgatcg cgtacgtgcc gtcgccgagg tcgacgacga
     1141 cgcggatctg gtcgttggcg agcgcgccct cggtcgcgac gaccggtccc gcgggcgcgg
     1201 gcgcgttgat cggcgtgaac gtcgcccacc cgaacccgtc gaccggaccg gtgtcgaaca
     1261 acaaccgccg ttggggcgcg accagcaggc gcaaccgcat cgaacggccc gcgctgccgc
     1321 gctgcaacag cgacgccttc agctccgcga gatcgatgcg cgtctcgccg gggccggctt
     1381 cctgcaacac gatgtcgtcg aagcggtcgt cgccgtggcc ggcgtcctcg acgatgtcgt
     1441 aggaggccac ctgccggccc gcgaactcgg tgccgcgcat gaggtcgagc acccagcgca
     1501 ccttctggcc ggtcaccatc gtcgagtagc cctcgcccga gatcgtgccg atgacctgcg
     1561 tcgggcgcgg cgtgccgtcg ggcccgacga agtgcacggg gccgtcaccg ggcagtgtgc
     1621 cctcgacgag cccggaacgg gtgcgcgccg tcgggttggc gacgaccgtc gaacccgcgg
     1681 gcgcgtcgac gtcgcgcgcg agcacgtgca tcgcgtcgcg cacgcacgtg tcgccgatct
     1741 ggcgcgcctc gtagtagcgg acgagcacct ggtcgacgac ctcgtcgtgg ctgcacgcgc
     1801 acgacgagtc gtgcgcactg ttgagcacga cactgcgcca cgcgagctcg aacaaccgcg
     1861 acggataccg tgccgcgggc gtgaacagcg cgtgcaacgg ctcggcccgc cgctccagca
     1921 cccgctccgc ggccgcgcac gcttggtgga cgtcgacgcg gttcgacgcg acccccatga
     1981 ggatgttggc ccgagccccc gaccgcagct cgcccttcac ctcggggaga tcgtcggacg
     2041 gctgcaacgg caggtactcg ggcagcgagg tgacgacgaa ctcgaagtcg tcctgctggt
     2101 cgttcgcctc ggcgacgacg cgtcccaacc acggctgggg catctggtgg tcggtgccgt
     2161 tcatgaacag catgtcgccg atgcgcgcgt cgcccagctc ctgctggtag tcgcgcgcgc
     2221 gtaacacgag acccttggcg tccgacggga tgtcgcgccc gttcgagtac gacccgtaca
     2281 ggtactcaca gcgcacacgt gagccgtcgg gtgactgcca ccagaacgcg gtcttgtcga
     2341 cggtgctcgg gataccgcgc cacgcgacgg cgtgctcgat gcccgcgagc gcaaggatct
     2401 gcggcatctg gccgatgtgg ccgaacaggt cggggaggta gccgatctcc atcgcgccgc
     2461 cgtactcggc ggcctgcgcg aaccccatct gcaggtcgcg cacgatcgtc tcgcccgaca
     2521 ccatgaactc gtccatctgg accatccacg gcccgacgtg cacgcgtccc gacgcgcaca
     2581 gccggcgcag cgcggcctcg gcctcggggc gcacttcgag gtagtcgtcg aggatcgccg
     2641 tctgcccgtc gagcaggaac cgcgcgtacg acgggtccga ttcgagcaac ggcaccaaca
     2701 cgtccagcag ccgcacgagg cgcatacgga aggtctggaa cggtgaatac cactcccggt
     2761 cccagtgcgt gtgcggaaca atcccaacgc gaatcgtcat ggcgctcagc ctaaggtccg
     2821 cggcatggcc ggatcactgg acggcaagat cgcgctggtc gcgggggcga cccgagcggc
     2881 cggacgaggc attgcggtgc agctcggcgc ggccggggcc accgtgtact gcacgggccg
     2941 gacgaccgcg gcccagcggt cggagatgaa ccggccggag acgatcgagg agacggccgc
     3001 gctggtggac gccgccggtg gccgcggcat cgcggtgcag gtcgaccacc tggtgcccga
     3061 ccaggtgcgc gcgctcgtcg aacgcatcga cgcggcgcac ggcgcgctcg acatcctcgt
     3121 gaacgacatc tggggcgtga agatcgagtg ggacaagccc gtgtgggaat cgacgctcga
     3181 caacgggctg cacacgttgc gcctcggcgt cgacacccac gcgatcacga gccactttgc
     3241 gatcccgctg atgttgaaga cgccgggcgg gctcgtcatc gagatgaccg acggcacgaa
     3301 cgagtacaac gcgacgaact atcgcgtgtc gttcttctac gacatcgcga aggcggccgt
     3361 gaaccgcatg gcgttcgcac tcgcgcacga gctcgcgcca aacggccgca ctgcggtcgc
     3421 gctcacaccg gggtggctgc gatcggagga aatgctcgac gcgtaccaag tcaccgagga
     3481 gaactggctc gacgcgacca agatccagcc gcacttcgcg atctcggagt cgcccgcgt
//
LOCUS       DASUGA010000855         2324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_488297, whole genome shotgun sequence.
ACCESSION   DASUGA010000855 DASUGA010000000
VERSION     DASUGA010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488297"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1465
                     /gene="infB"
                     /locus_tag="VFX21_16850"
     CDS             <1..1465
                     /gene="infB"
                     /locus_tag="VFX21_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX5097695.1"
                     /translation="NANVVAGEAGGITQHIGAYRVVKNDRKLTFIDTPGHEAFTAMRS
                     RGAQVTDIAILVVAADDGVMPQTVEAINHARAAEVPIVVAVTKSDREDADTTRVRQQL
                     TEQELVPEEWGGDTIVMDVAAPINVGVDELLDSLVLVADAEIAEDLVANPKKPGKAYV
                     LEANLDAGRGPVVTALVEEGTIRVGDTIVAGQAWGRIRAMFDEHGQQVTEAGPSVPVE
                     VLGLDDVPLAGDELRVAPSDKIARTVAEARAYRRRAAAQRSTMMLSGGARLEDVFALV
                     QRGEVATLNLIVKADVQGSLEALTDALRKLDQEHDEVRLSFVHRGVGGITESDLNLAV
                     VSNATVIGFNVRPDRNAREVAEREGVEIRLYEVIYKLLEDVNNALVGMLKPEFEEVVT
                     GDAEVREIFSVPRVGRIAGCFVTNGVITRGSKVRFLREGTVIWKGAIASLRRFKDDVR
                     EVREGFECGIGLENYQDLKPGDVIETYEDREIARVAG"
     gene            complement(1791..>2324)
                     /locus_tag="VFX21_16855"
     CDS             complement(1791..>2324)
                     /locus_tag="VFX21_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097696.1"
                     /translation="TPITAAPATTTTSAPATTTTTVPSPLFSFDGSVPAPDLVNSGTD
                     YEAIYRSLDAYAQWINAHHPHPAAVDEIYVEGTPLYDAAVSDMHKLLQYRYRWYRTAS
                     VLTTVEVVGATSDLVVLRVNYAQDRTVVVRSDGGVSDDQQLPASSTRTVTLRLDEKGH
                     WRIESSEGDAEIHLRQT"
BASE COUNT          416 a          743 c          781 g          384 t
ORIGIN      
        1 caacgcgaac gtcgtcgcgg gcgaagccgg tggcatcacg cagcacatcg gtgcgtatcg
       61 cgtcgtcaag aacgaccgga agctcacctt catcgacacg ccgggtcacg aggcgttcac
      121 cgcgatgcgt tcacgcggcg cgcaggtcac cgacatcgcg atcctcgtgg tcgcggccga
      181 cgacggcgtc atgccgcaga cggtcgaagc gatcaaccac gcgcgtgcgg ccgaagtgcc
      241 gatcgtcgtg gcggtcacca agtcggaccg tgaagacgcc gacaccaccc gcgtgcgcca
      301 gcagctcacc gagcaggagc tggtgccgga ggagtggggc ggcgacacga tcgtcatgga
      361 cgtcgcggcg ccgatcaacg tcggtgtcga cgagctcctc gactcgttgg tgctcgtcgc
      421 cgatgccgag atcgccgaag acctcgtcgc caacccgaag aagccgggca aggcgtacgt
      481 gctcgaagcg aacctcgacg ccggccgcgg cccggtcgtg accgcgctgg tcgaggaagg
      541 caccatccgt gtcggtgaca cgatcgtggc cgggcaggcc tggggtcgca tccgcgcgat
      601 gttcgacgag cacggccagc aggtcaccga agccggtccg tcggtgccgg tcgaggtgct
      661 cggtctcgac gacgtccccc tcgccggaga cgaactgcgc gtggcgccgt ccgacaagat
      721 cgcccgcacc gtcgccgaag cgcgtgcgta ccgccgtcgc gcggccgcgc agcgttcgac
      781 gatgatgctc tcgggcggtg cacggctcga agacgtgttc gcgctggtgc agcgcggcga
      841 ggtcgcgacg ctgaacctga tcgtgaaggc cgacgtgcag ggttcgctcg aagcgttgac
      901 cgacgcgctg cgcaagctcg atcaggagca cgacgaagtt cgcttgtcgt tcgtgcaccg
      961 cggggtcggc gggatcaccg agtccgactt gaacctcgcg gtcgtgtcga acgcgacggt
     1021 gatcggcttc aacgtgcgtc ccgaccgcaa cgcccgtgag gttgccgagc gcgaaggcgt
     1081 ggagatccgt ctctacgagg tcatctacaa gctgctcgaa gacgtgaaca acgcgctcgt
     1141 cggcatgctc aagcccgagt tcgaagaggt cgtcaccggc gacgccgagg tccgcgagat
     1201 cttcagcgtg ccgcgtgtcg gccgcatcgc cggttgcttc gtgaccaacg gtgtgatcac
     1261 ccgcggttcg aaggtccgct tcctgcgcga gggcacggtc atctggaagg gcgcgatcgc
     1321 atcgctgcgc cgcttcaagg acgacgtccg tgaagtccgc gagggcttcg aatgcggcat
     1381 cggcctcgag aactaccaag acctcaagcc cggcgacgtc atcgaaacct acgaagaccg
     1441 agagatcgcc cgagtcgccg gctaagtgcc cgcgtacctg cgtccctgaa ccacccgagc
     1501 ggtggtcgag cggcgcaggt atgagcatga gccaacccac gtcacgacga agacgaccgg
     1561 tccggccatc ccaccgaacg cgaaccaacg gttgtgcacg ccacgtcttc ggagttgaga
     1621 ccgccaagag atggcaaaaa gcgcgtccgc ggacgcgtcc gcgtcgagtc gtggtcacga
     1681 aattcgcaac atcctgcgaa tttggtgacc acgagaaacc tgtgcgggcg actccagatt
     1741 ctcgactcgt accccgcgcc ctaggaccac cgggacggtg gttgaacggg ctacgtctgc
     1801 cgaaggtgaa tctccgcatc gccctccgac gactcgatgc gccagtgtcc cttctcgtcc
     1861 aagcggagcg tcaccgttct ggtcgatgac gccgggagct gttggtcgtc gctgacgcct
     1921 ccgtcgcttc tgacgacaac agttcggtcc tgtgcgtagt tgactcgaag cacaaccagg
     1981 tcactcgttg ccccgacgac ttcgacagtc gtgagaacgg aagctgttct gtaccagcgg
     2041 taccggtatt ggagcagctt gtgcatgtcg gaaaccgccg cgtcgtagag tggcgtgcct
     2101 tcgacgtaga tctcatctac cgcagccgga tggggatgat gcgcgttgat ccactgcgcg
     2161 tacgcgtcga ggctacggta gatcgcttcg tagtcggtgc cgctgttgac gaggtccggt
     2221 gcgggaacgg acccgtcgaa gctgaagagg ggcgatggca ctgtggtcgt ggtggtcgcc
     2281 ggcgccgagg tcgtcgttgt cgctggagca gccgttatcg gagt
//
LOCUS       DASUGA010000856         5076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_524771, whole genome shotgun sequence.
ACCESSION   DASUGA010000856 DASUGA010000000
VERSION     DASUGA010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524771"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..814)
                     /locus_tag="VFX21_16860"
     CDS             complement(68..814)
                     /locus_tag="VFX21_16860"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5097697.1"
                     /translation="MSTPYTFGDSELARERLGLVAAAFEAPTRTLLDDVPGRFRRYVL
                     DLGCGPGYTTALLAQKFVLAQVTGYDAAPAMLAEARRRVPRAWFVKADVTEPLLLPAD
                     IVYGRLLLGHLREPAHVLDTWAAALRPGNGILVCEEPVRYRSDDRRFARYEEIVTDVV
                     ARTGATLWAATMLDATPEGCTRVLDRVVEHPVPAGDAAAMFWRNAVQWGEGIPDVPGL
                     IDEFRALEADGATEPVTWEIRQTIWLRTLA"
     gene            complement(811..1485)
                     /gene="purQ"
                     /locus_tag="VFX21_16865"
     CDS             complement(811..1485)
                     /gene="purQ"
                     /locus_tag="VFX21_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX5097698.1"
                     /translation="MTARIGVVLFPGSNCEQDIVWSVEQLGATADILWHGDATTKGVD
                     AVVVPGGFAHGDYLRTGAIARFSPVMQAVGEFAAAGGPVVGICNGFQILTEAQLLPGA
                     LQKNAGLKFLCETVELRVETTNTVLTSEAHVGDILRVPINHFEGNYVCDEATLAVLRA
                     DDRVVVRYVDNPNGSLDDIAGICNEGRNVVGLMPHPERASDVLLGSDDGVVLLRSLLA
                     ACTTRV"
     gene            complement(1482..1745)
                     /gene="purS"
                     /locus_tag="VFX21_16870"
     CDS             complement(1482..1745)
                     /gene="purS"
                     /locus_tag="VFX21_16870"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015170298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX5097699.1"
                     /translation="MSAYEARVDIAHLPGISDPAGATVERALPALGYTNVSQVHIGKT
                     IRLVIDAADADAAHAQVAEMCERLLANPVIESYDVTISELAAS"
     gene            complement(1769..2644)
                     /locus_tag="VFX21_16875"
     CDS             complement(1769..2644)
                     /locus_tag="VFX21_16875"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEX5097700.1"
                     /translation="MVLRILAGLTALVLVTGACGRDDDDQASDGQPRVVAAFSPVAWA
                     ADQIGGGAVAVQNLTPAGAEPHDLELTTGDVDDIEDADLVIVMGHDFQPGVEDAADRR
                     SSGTLRLLDHLDVDTDDPHVWLDPIVMGDIVDEIERALATVDPPHASEFAANAAALHA
                     ELDTLDGEYRSGLGDCERDLIVTSHEAFGHLARRYGLEQHAIAGVDPEQEPNANRIAE
                     LADLVEEHGVTTIFTEELVSPRVAESLAREAGVQTETLNPLEGLTDDERAAGDDYLSV
                     MRANLEKLRTALGCR"
     gene            complement(2677..3651)
                     /locus_tag="VFX21_16880"
     CDS             complement(2677..3651)
                     /locus_tag="VFX21_16880"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5097701.1"
                     /translation="MSELGDALFARRREISDRVLTSWHDRSPDAADAADLRVRDDILR
                     TTELATLAVAQYLTARQLQSEEQARVVAATGKAPLRDTIALAELAKLYLYWRDITISV
                     LADEAEGRGFDADVHRDAATIVRAGSDGSIIRMAKQFDAERGRLQRELSIEQARLAHH
                     AFHDVLTGLPNRRLFFDRLAHALDVRTREQRGLAVLFVDVDRFKAVNDRCGHEGGDRV
                     LVVVAERLLAAVRRCDTVARLGGDEFVVLCEGLDDGVDEASLMAERIIRSVDQPVESE
                     GLAGVSVSVGVAIPGPSDDADRLLRRADAAMYRAKVAGPGRYECVAAP"
     gene            complement(3815..4699)
                     /locus_tag="VFX21_16885"
     CDS             complement(3815..4699)
                     /locus_tag="VFX21_16885"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX5097702.1"
                     /translation="MSDQTALPHLYSGKVRELYEVGHDRLLMVATDRVSVFDVVLPDA
                     IPDKGRVLTGLSYFWFEHTKDLVPNHVISADPTDFPETAGPVDGRAMLVRATRPVQME
                     CIVRGYCFGHGWKEYADVGTVGGFRVPAGLREAERLPEPLFTPSTKTDEGHDVPIDKD
                     EAIALVGAEQYEKLRDMSLALYEYGARNAEECGILLADTKFEFGELDGKLLVIDEMMT
                     PDSSRYWPRDEYRVGGSPPSFDKQYVRDFMDATGWNHEPPAPNLSPEAIANTQARYRE
                     CYEQLTGESLDDWFRPEH"
     gene            complement(4707..>5076)
                     /locus_tag="VFX21_16890"
     CDS             complement(4707..>5076)
                     /locus_tag="VFX21_16890"
                     /inference="COORDINATES: protein motif:HMM:NF021883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX5097703.1"
                     /translation="IPDSLMLAYYLTVKFTNIARDMRVYPERMLRNIDSSFGLVFSQP
                     VLLALVESGLTRDDAYRLVQRNAMKAWQEERSFRDVLTDDAEVTAALDDARLDACFDL
                     KRALSNTGHVFDALDALGDL"
BASE COUNT          769 a         1725 c         1762 g          820 t
ORIGIN      
        1 aagggcatct cctacggcgc ctggcgggag ttcggcgtcg cccccgaggt gctgaaaaag
       61 gccggcatca cgcgagggtc ctgagccaga tcgtctgacg gatctcccac gtcaccggtt
      121 cggtcgcgcc gtcggcttcc agggcgcgga actcgtcgat cagaccgggc acatcgggaa
      181 tcccttctcc ccattgcacg gcgttgcgcc agaacatcgc cgccgcatcc ccggccggca
      241 ccgggtgctc gacaacccgg tcgagcaccc gcgtacagcc ctcgggtgtc gcgtcgagca
      301 tggtcgcggc ccacagcgtc gcgcccgttc gcgcgacgac gtcggtgacg atctcctcgt
      361 agcgcgcgaa gcgccggtcg tcgctgcggt agcgcaccgg ttcctcgcac acgaggatgc
      421 cgttgcccgg ccgcagtgcc gccgcccacg tgtcgagcac gtgcgccggt tcgcgtagat
      481 gaccgagcag caagcggccg tagacgatgt cggccggcag cagcaacggc tcggtgacat
      541 cggccttcac gaaccacgcg cgcggcaccc gccggcgcgc ttcggccagc atcgcgggcg
      601 ccgcgtcgta ccccgtcact tgtgcgagga cgaatttctg cgcgagcagc gcggtcgtgt
      661 aaccgggacc gcagcccagg tcgagcacgt aacgacgaaa ccggccgggg acgtcgtcga
      721 gcaacgtgcg cgtcggcgcc tcgaacgccg ccgcgaccag cccgaggcgc tcgcgcgcga
      781 gctctgagtc gccgaacgtg tacggcgtgc tcacacccgg gtcgtgcagg cggcgagcaa
      841 cgaccgcagg agaacgacgc cgtcgtcgga gccgagcagc acgtcggagg cgcgttcggg
      901 gtgcggcatc aggccgacga cgttgcggcc ctcgttgcag atgccggcga tgtcgtcgag
      961 gctgccgttc gggttgtcga cgtaacgcac gacgacgcgg tcgtcggcgc gcaacacggc
     1021 cagcgtcgcc tcgtcgcaca cgtagttgcc ctcgaagtgg ttgatcggta cccgcaagat
     1081 gtcaccgacg tgcgcctcgc tcgtcagcac cgtgttcgtc gtctccaccc gcagctcgac
     1141 ggtctcgcac aggaacttca ggcccgcgtt cttctgcagt gcgcccggga gcagctgcgc
     1201 ttcggtgagg atctggaagc cgttgcagat gccgacgacc ggaccacccg cggccgcgaa
     1261 ctcgccgacg gcctgcatga ccggagagaa gcgcgcgatc gcgccggtgc gcaggtagtc
     1321 gccgtgcgcg aaaccaccgg gcacgacgac cgcatcgacg cccttcgtcg tcgcgtcacc
     1381 gtgccagagg atgtccgcag tcgcgcccaa ctgctcgacc gaccacacga tgtcctgctc
     1441 acagttggag cccgggaaga ggacgacgcc gatccgcgcg gtcacgatgc ggcgagctcc
     1501 gagatggtga cgtcgtagga ctcgatgacc gggttcgcga gcaaccgttc gcacatctcg
     1561 gcgacttgcg cgtgggcggc atcggcgtcg gccgcgtcga tcacgaggcg gatcgtcttg
     1621 ccgatgtgga cttggctcac gttcgtgtag ccgagcgcgg gcagcgcccg ttcgaccgtg
     1681 gcgccggccg gatcggagat gccgggcagg tgggcgatgt cgacgcgggc ttcgtaggcg
     1741 gacacgtccc gatgctaacc gccgccggtt accggcaacc gagggctgtg cgcagctttt
     1801 cgaggttcgc gcgcatcacc gagagatagt cgtcgcccgc ggcgcgctcg tcgtcggtga
     1861 gtccttcgag cgggttcaag gtctcggtct gcacgccggc ttcgcgcgcc agagactcgg
     1921 ccaccctggg cgacaccagt tcttcggtga agatggtcgt gacgccgtgt tcctcgacca
     1981 gatcggcgag ctcggcaatg cggttcgcgt tgggttcctg ttcggggtcc acacccgcga
     2041 tcgcgtgttg ttccagcccg taccgccgcg cgagatggcc gaacgcctcg tgcgacgtca
     2101 cgatcaagtc gcgctcgcag tcgccgagtc cggagcggta ctcgccgtcg agcgtgtcga
     2161 gctcggcgtg gagcgcggcc gcatttgcag cgaactccga cgcgtgcggc gggtcgaccg
     2221 tcgcgagggc gcgctcgatc tcgtcgacga tgtcacccat cacgatcgga tcgagccaca
     2281 cgtgcggatc gtcggtgtcg acgtcgaggt gatcgagcaa ccgcagtgtg cctgacgatc
     2341 gccggtcggc cgcgtcctcg accccgggtt ggaagtcgtg gcccatcacg atcacgagat
     2401 cggcgtcctc gatgtcgtcg acgtcgcccg tcgtcagctc gaggtcgtgg ggttcggcgc
     2461 ccgcgggcgt gaggttctgc acggcgacgg cgccgccgcc gatctggtcg gccgcccacg
     2521 cgaccggaga gaacgcggcg acgacccgag gctgcccgtc ggaggcttgg tcgtcgtcgt
     2581 cacggccaca tgcgcccgtg accaggacca acgcggtcag accggccagt attctgagaa
     2641 ccattctcag atgctactcg atcgcgcggc cgcccgtcag ggcgcggcga cgcactcgta
     2701 gcgccccggc cccgcgacct tcgctcggta catcgccgca tcggcgcgcc gcaggaggcg
     2761 atcggcatcg tcgctcgggc ccgggatcgc gacgccgacg ctcaccgaga cgccggccag
     2821 gccctctgac tcgacaggct ggtcgacgct gcggatgatg cgctccgcca tgagcgacgc
     2881 ttcatcgacg ccgtcgtcga gcccttcgca caacacgacg aactcgtctc ctccgagtcg
     2941 ggcaaccgtg tcgcaacgcc gcacggcggc aagcagccgc tcggcgacga cgacgaggac
     3001 gcggtcgcct ccttcgtggc cgcatcggtc gttgacggct ttgaagcggt cgacgtcgac
     3061 gaacagcacg gccagacccc gctgttcacg ggtgcggacg tcgagcgcgt gcgcgagccg
     3121 gtcgaagaag agccgccgat tcggcagacc ggtcagcacg tcgtggaacg cgtgatgtgc
     3181 caagcgggcc tgctcgatgc tgagctcgcg ttgcagacgg cctcgctcgg catcgaactg
     3241 cttcgccatg cgaatgatgc tgccgtcgga cccggcccgg acgattgtcg cggcgtcgcg
     3301 gtgcacgtcg gcatcgaagc ccctgccttc ggcttcgtcg gcgagcaccg agatcgtgat
     3361 gtcccgccaa tacaggtaga gcttcgcgag ctcagccagc gcgatcgtgt cgcgcagcgg
     3421 cgctttgccg gtcgcggcca cgacgcgggc ctgctcctcg ctctgcagtt ggcgcgcggt
     3481 gaggtactgc gccacggcga gtgtcgcaag ctcggtcgtc cgcagaatgt cgtcccgcac
     3541 tcgcaggtcg gccgcgtcgg ccgcgtccgg cgaacggtcg tgccagctgg tcagcacgcg
     3601 gtccgagatc tcgcgccgcc gggcgaacag cgcatcgccg agctcggaca aacaccctga
     3661 acgggacatg tcgggacccg gtacgactgc tgtttcgccg tgcgggctgt tcgacatgct
     3721 cttcggatcg gcgcccgagc gtccggtctt gacggcatct gtggccgttc cgcgcaccgc
     3781 cgggtggcac cccggcggcg ctagttccgg ccggctagtg ctcggggcgg aaccagtcgt
     3841 cgaggctctc gccggtgagt tgttcgtagc actcgcggta gcgcgcctgc gtgtttgcga
     3901 tcgcttccgg tgagaggttc ggcgcgggcg gctcgtggtt ccagccggtc gcgtccatga
     3961 agtcgcgcac gtactgcttg tcgaacgacg gcggggagcc accgacgcgg tattcgtcgc
     4021 gcggccagta gcgcgaggag tcgggcgtca tcatctcgtc gatcacgagc agcttgccgt
     4081 cgagctcgcc gaactcgaac ttggtgtcgg cgagcaggat gccgcactcc tcggcgttgc
     4141 gcgcgccgta ctcgtacaac gcgagcgaca tgtcgcgcag cttctcgtac tgctctgcgc
     4201 cgacgagtgc gatcgcttcg tccttgtcga tggggacgtc gtgaccttcg tcggttttgg
     4261 tcgacggcgt gaacaagggt tccggaaggc gctccgcctc ccgcaagccc gccggcacgc
     4321 ggaacccgcc gaccgtgccg acgtcggcgt attccttcca gccgtgtccg aagcagtagc
     4381 cgcgcacgat gcactccatc tgcaccggac gggtcgcgcg cacgagcatg gcgcgcccgt
     4441 cgacgggtcc cgcagtctcg gggaagtcgg tcgggtcggc ggagatgacg tggttgggca
     4501 cgaggtcctt ggtgtgctcg aaccagaagt acgacaggcc ggtgagcacg cggcccttgt
     4561 cggggatcgc gtcggggagc acgacgtcga acaccgagac gcggtcggtg gcgaccatca
     4621 acaaccggtc gtgaccgacc tcgtacagct cgcggacctt gcccgagtac agatgcggca
     4681 gcgcggtctg gtcgctcaca gccgtcctag agatcgccca gcgcgtcgag ggcatcgaac
     4741 acgtggccgg tgttcgacag cgcgcgcttg aggtcgaagc acgcgtcgag gcgcgcgtcg
     4801 tccagtgcgg ccgtgacttc ggcgtcgtcg gtgagcacgt cgcggaacga gcgttcttcc
     4861 tgccatgcct tcatcgcgtt gcgctgcacc agtcggtacg cgtcgtcgcg ggtgagcccg
     4921 gactcgacga gcgcgagcaa caccggctgg ctgaacacca gaccgaacga cgagtcgatg
     4981 ttgcgcagca tccgctcggg atacacgcgc atgtcgcggg cgatgttcgt gaacttgacc
     5041 gtcaggtagt aggcgagcat cagcgagtcg gggatg
//
LOCUS       DASUGA010000857         3809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_106835, whole genome shotgun sequence.
ACCESSION   DASUGA010000857 DASUGA010000000
VERSION     DASUGA010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106835"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VFX21_16895"
     CDS             complement(<1..558)
                     /locus_tag="VFX21_16895"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX5097704.1"
                     /translation="MATGTRPRDWADVDYYAVLGIAEDADDETIGRAFRTLAKQLHPD
                     AGATERDVERFKDVAGAYEVLGDRKIRREYDRVRAELAAARDERIAYGNRSRVADPFA
                     PRDYPPGRPMPAFAARRVRPRRGFTRRRAWLAAIGGLVIFAGGVVVGLVTLSLHYADA
                     RIHDDTVATRVTDVTNNGRREISFVT"
     gene            complement(588..809)
                     /locus_tag="VFX21_16900"
     CDS             complement(588..809)
                     /locus_tag="VFX21_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097705.1"
                     /translation="MPIDQIRVDPADYPAEEMPCLRCGEPAMLRFAGPCPACADELRA
                     TMRSEGQAVDTEYVPRMNVTPNAVALKDD"
     gene            complement(907..1767)
                     /locus_tag="VFX21_16905"
     CDS             complement(907..1767)
                     /locus_tag="VFX21_16905"
                     /inference="COORDINATES: protein motif:HMM:NF014951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097706.1"
                     /translation="MPDVRLVTATDLPVPDTDMPLVVSALRDRGAIVAVDDWRAAAVD
                     WADARVTVVRSPWDYVDHLAEFLAWVDRVDAVTNLWNPAAVLRWNTHKSYLLDLGARG
                     APVVPTVMLTHGGAAALDGIADAQGWNALVVKPAVGVGGDGAGRFDVGDPAGQRHLDD
                     LLRAGDVLVQQFAPAIVSDGEYSVVMFDGVVSHALRKRVASGEFRIHEERGGRTDECT
                     PTAGQVELAERLWRALPASALYARMDMVQLAGQWHVLEVEATEPALWLDQVSPAATDR
                     LADAVMARLR"
     gene            1771..2490
                     /locus_tag="VFX21_16910"
     CDS             1771..2490
                     /locus_tag="VFX21_16910"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX5097707.1"
                     /translation="MILAVLVAAALTMAAGCSSGVDDGAATDTTDTPTTTLAETTTVA
                     PTTPTSIAGVAARVDPVTKEQLGASWHEGCPVAAEDLRLLTIPYIGFDGTVHAGQMVV
                     HADVVTDVLQVFQRLYSTGFPIEKMQLVTEYDADDDKSTKANNTSGFNCRQITEGGGW
                     SQHAYGRAVDINPVQNPYVYADGHVLDPAAEPYLDRSNTGPGVIHDGDVVVQSFKELG
                     WGWGGDYETRKDYQHFSTTGG"
     gene            2574..2810
                     /locus_tag="VFX21_16915"
     CDS             2574..2810
                     /locus_tag="VFX21_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097708.1"
                     /translation="METIWEPIEGVDLDTYARITAEILKGDVCPVDIQRFVAAYFGVP
                     EGRWPSISARWVQRLVEHPELRAQYSNVYAATRS"
     gene            complement(2797..3414)
                     /locus_tag="VFX21_16920"
     CDS             complement(2797..3414)
                     /locus_tag="VFX21_16920"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5097709.1"
                     /translation="MTAPLLAGLDVSGTPDAWRAAGFAVDDDGGIRIGHVRMQTECGT
                     KGIAGWSFAGLPHAGDIDGLATATCDGPPIEPGEHPNGATLIDHVVVFSGDAGRTTRA
                     LEAMGLDARRQRDAGTYGSPMRQTFFKTGDVIIELIGPVEPEPEPGPTGFYGIACSVK
                     DLDACKALLGDALGNIKDAVQPGRRIVTLRHRDVGLRVATAFMSE"
     gene            complement(3411..>3809)
                     /locus_tag="VFX21_16925"
     CDS             complement(3411..>3809)
                     /locus_tag="VFX21_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5097710.1"
                     /translation="WRDDIAEYHGEFVDFPPTWSWPKPNGGRVPVLIGGAPGPKLFAH
                     VAEYADGWMPIGGSGIRSSLPALQAACAARGRELKELRIVPFGTIPDPGKVEYYASLG
                     IEEVVLRLPGGDADRVLPVLDKYATLAEDS"
BASE COUNT          587 a         1332 c         1330 g          560 t
ORIGIN      
        1 ggtgacgaac gagatctcgc gccgaccatt gttggtgacg tcggtgacac gcgtcgcgac
       61 ggtgtcgtcg tggatgcgcg cgtcggcgta atggagcgag agcgtcacca acccgaccac
      121 gacaccgccc gcgaagatga cgagaccacc gatggccgcg agccacgcgc gccggcgggt
      181 gaagccccgg cgcggtcgca cgcggcgcgc cgcgaacgcc ggcatcggcc ggcccggcgg
      241 atagtcgcgt ggcgcgaacg gatcggcgac ccgcgaccgg ttgccgtagg cgatgcgctc
      301 gtcgcgggcg gccgccagct cggcgcgcac ccggtcgtac tcacggcgga tcttgcggtc
      361 gccgagcacc tcgtaggccc cggcgacatc cttgaagcgc tcgacgtcgc gttcggtcgc
      421 gccggcgtcg gggtgaagct gcttcgcgag ggtgcggaag gcgcgtccga tcgtctcgtc
      481 gtcggcgtct tcggcgatgc cgaggactgc gtagtagtcg acgtcggccc agtcacgcgg
      541 ccgtgtcccg gtcgccatac gcgcgggaga ataccgccgt cttcgattca gtcgtccttc
      601 agggcaacgg cattgggggt cacgttcatc ctcgggacgt actcggtgtc gacggcctga
      661 ccctcggacc gcatcgtcgc ccgcaactcg tccgcgcacg cggggcacgg tcccgcgaac
      721 cgcagcatcg ccggctcgcc gcagcgcagg cacggcatct cttcggccgg gtagtcggcg
      781 gggtcgacgc ggatctggtc gatcggcatg aaatcgtttc ctttgcgaag acgtcagaag
      841 atcgcggcga gcaacagccc ggcgagcgcg cagatcgcga gcacccgcgg atcgtagagg
      901 ccgcggtcag cgcaatcggg ccatcaccgc gtcggccagg cgatccgtgg ccgcgggcga
      961 gacctggtcg agccagagcg ccggttcggt cgcctcgacc tcgagcacgt gccactggcc
     1021 cgccaactgc accatgtcca tgcgcgcgta gagcgcggat gccggcagcg cgcgccacag
     1081 ccgctcggcc aactcgacct gtccggcggt cggcgtgcac tcgtccgtgc gcccaccgcg
     1141 ctcttcgtgg atgcggaact cccccgacgc gacccgcttg cgcaacgcgt gcgagacgac
     1201 gccgtcgaac atgacgaccg agtactcccc gtcgctcacg atcgcgggcg cgaactgttg
     1261 caccagcacg tcaccggcgc gcagcaggtc gtcgaggtgt cgttggcccg cgggatcgcc
     1321 gacgtcgaag cggcccgcgc cgtcgccacc gacaccgacc gcgggcttca caaccagggc
     1381 attccaccct tgggcgtcgg caatgccgtc cagcgcggcc gcgccgccgt gggtcagcat
     1441 cacggtcggc acgacgggcg cgccccgcgc gcccaggtcg agcaggtagg acttgtgggt
     1501 gttccaccgg agcacggcgg ccggattcca caggttcgtg accgcgtcca cccgatcgac
     1561 ccacgcgagg aattcggcca gatggtccac gtagtcccac ggcgaacgca ccacggtcac
     1621 tcgcgcgtcg gcccagtcga cggcggcggc gcgccagtcg tcgaccgcga cgatcgcgcc
     1681 ccggtcgcgc agcgcggaga cgacgagggg catgtcggtg tcggggaccg gcaggtcggt
     1741 cgccgtcacg aggcgcacat cgggcatcgg atgattctgg ccgttctcgt cgcggcggcg
     1801 ctgaccatgg cggccggatg ctcgtccggc gtcgacgacg gcgccgcgac cgacaccacc
     1861 gacacgccga cgacgaccct ggccgagacg acgacggtcg cgcccaccac accgacgtcg
     1921 attgcgggtg tcgcggcgcg cgtcgatccg gttacgaagg aacagctcgg cgcgtcgtgg
     1981 cacgaagggt gcccggtcgc ggccgaagac ctgcgcctgc tgacgatccc ctacatcggg
     2041 ttcgacggca cagtgcacgc aggccagatg gtcgtgcacg cggacgtcgt gaccgacgtc
     2101 ttgcaagtgt tccagcgttt gtacagcacg gggttcccga tcgagaagat gcagctcgtg
     2161 accgagtacg acgccgacga cgacaagtcg acgaaggcga acaacacgtc cgggttcaac
     2221 tgccgccaga tcaccgaagg cggcggctgg tcgcagcatg catacggccg ggccgtcgac
     2281 atcaacccgg tgcagaaccc ctatgtgtat gccgacgggc acgtgctcga cccggccgcg
     2341 gagccgtacc tggaccgctc gaacacgggt cccggtgtca tccacgacgg cgacgtcgtc
     2401 gtgcagtcgt tcaaagagct gggctggggc tggggcggcg actacgagac ccgcaaggac
     2461 taccagcact tctccaccac cggtggctaa tgctggtggc cagggccctc cggcccaagt
     2521 tttgagtaac gacgactcat gtcgcgggcg gcgggttccc gcatgctcgt cccatggaaa
     2581 cgatctggga gccgatcgag ggcgtcgacc tggacaccta cgcccgcatc acggccgaga
     2641 tcctgaaggg tgacgtgtgc ccggtcgaca tccagcggtt cgtcgccgcg tacttcggcg
     2701 tccccgaagg ccggtggccc tcgatctccg cacggtgggt gcagcgactg gtcgagcacc
     2761 cggagctgcg ggcgcagtac agcaacgtct acgccgctac tcgctcatga aagccgtggc
     2821 gacgcgcagt ccgacgtcgc ggtggcgcag cgtcacgatg cgacgacccg gttggacggc
     2881 gtctttgatg ttgccgagcg cgtcgccgag cagtgctttg cacgcgtcga gatctttgac
     2941 cgagcacgcg atgccgtaga agccggtcgg gccgggttcc ggttcgggtt cgacgggtcc
     3001 gatcaactcg atgatcacgt cgccggtctt gaagaacgtc tggcgcatcg gcgacccgta
     3061 cgtcccggcg tcgcgttggc ggcgcgcatc gagtcccatc gcttccagcg cgcgcgtggt
     3121 gcggcccgcg tcgccggaga acacgacgac gtggtcgatg agcgtcgcgc cgttgggatg
     3181 ctcccccggt tcgatcggcg gaccgtcgca cgtcgcagtc gcgaggccgt cgatgtctcc
     3241 cgcgtgcggg aggcccgcga acgaccagcc ggcgatgccc ttcgtgccgc actcggtctg
     3301 catgcggacg tggccgatcc ggatgccgcc gtcgtcgtcg accgcgaaac cggccgcgcg
     3361 ccacgcgtcc ggcgttccgg agacgtcgag gccggcgagc agcggtgcgg tcatgagtcc
     3421 tccgcgagcg tcgcgtactt gtcgagcacg ggcagcacgc ggtcggcgtc gccgcccggg
     3481 agccgcagga cgacctcttc gatgccgagc gacgcgtagt actcgacctt gcccgggtcg
     3541 gggatcgtcc cgaacggcac gatccgcagc tccttcagct cacgcccgcg cgctgcgcac
     3601 gccgcttgca gcgccggcag cgaggaacgg atgccgctcc caccgatcgg catccatccg
     3661 tccgcgtact cggcgacgtg cgcgaacagc tttggtccgg gcgcaccgcc gatgaggacg
     3721 ggcactcgtc cgccgttggg tttgggccac gaccacgtcg gcgggaagtc gacgaactcg
     3781 ccgtggtact ccgcgatgtc gtcgcgcca
//
LOCUS       DASUGA010000858         3124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_905517, whole genome shotgun sequence.
ACCESSION   DASUGA010000858 DASUGA010000000
VERSION     DASUGA010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905517"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VFX21_16930"
     CDS             <1..355
                     /locus_tag="VFX21_16930"
                     /inference="COORDINATES: protein motif:HMM:NF014418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX5097711.1"
                     /translation="RIVAKVAAEHPTLRTTGGKMILELRPDIAWDKGKALARIMEVAG
                     LDSDGMLPVYIGDDVTDEDALLAVRARGLGIVVGSGGRQTAAHDRLDDTEAVTEFLAR
                     LIDHVDRLDDDGRSS"
     gene            352..2748
                     /locus_tag="VFX21_16935"
     CDS             352..2748
                     /locus_tag="VFX21_16935"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEX5097712.1"
                     /translation="MNPWLWSYHGYEPDEEGVREALCALGNGYLATRGALPESHADGA
                     HYPGTYIAGVFNRLRATVEGQTVENESLVNAPNWLAFTLTGPDGTTFECGTTEVLDHE
                     LELDLRRSLLTRRSRLRDANGRVLSITQRRFVSLRDSHLAALETTLVAENWSGRLRVE
                     SALDGRVTNSGVRRYAALGTDHLVGQVARQISDDAVALDVQTVDSHVRIAMAARTTVD
                     VPTVMVDRDVVTDDRYVAHVLDLELQEGAPVVVDKIVAIYTSRDDGIYEPLSESEDAV
                     CHAPSFDDLLERHVVSWNHAWERGRIDITDGQGHTGRVLNLHIFHMLQTTSKNTAELD
                     VGVPARGLHGEAYRGHIFWDELFIFPFLNWRVPELSRALLRYRSRRLDRARRNAAEAG
                     YRGALYPWQSASSGREETQTLHLNPKSGRWLPDASHLQHHINGAVAYNVWQYWQVTAD
                     IEFMRFWGTEMLLEIARFWSSMATYNHTLDRYEIKGVMGPDEYHEAYPHADTPGLNNN
                     AYTNVLAVWCLIRAFDALALLPESRRRQLREKLVLTSEELDRWGDVSRKMRLCFHDGV
                     LSQFEGYDQLEELDWEDYRARYGNIQRLDRILESEGLSTNNYKLAKQADTLMLFYLFS
                     EKELSQLIERLGYEYPPDFLRRHLEYYEPRTSHGSTLSQTVASFVFSRIDRERSWDLF
                     REALQSDVTDSQGGTTAEGIHLGAMAGTVDLVQRCYTGIETRDDILRLDPVLPVELGS
                     LSFDVRYRGHAMHLEFTADYVRIKSEREGAAVDVPMPVDVCGTIYEIIPGATLEVKLG
                     "
     gene            complement(2761..>3124)
                     /locus_tag="VFX21_16940"
     CDS             complement(2761..>3124)
                     /locus_tag="VFX21_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase type 11"
                     /protein_id="HEX5097713.1"
                     /translation="TWAPRDPLLERWNEVYHGITQRNRAEADAGRFLLGWVRAAGFEI
                     EHVGSSTWTFADPDSRHWWGDLWADRVLLSSYAEQAVDYGLADKDELARIADAWRRWS
                     EEPDGFFAVLHGEVIGRA"
BASE COUNT          545 a         1046 c         1030 g          503 t
ORIGIN      
        1 gaggatcgtg gcgaaggtcg cggccgagca tccgacgctc cgcacgaccg gcggcaagat
       61 gatcctcgag ctgcggcctg acatcgcgtg ggacaagggg aaggcgctgg cccgcatcat
      121 ggaggtcgcc gggctcgact ccgacgggat gctgcccgtc tacatcggcg atgacgtcac
      181 cgacgaggat gcgctgctcg ccgtgcgtgc gcgtgggctc ggcatcgtcg tgggctccgg
      241 tggccggcag accgcggccc acgaccgact cgacgacact gaggcggtga ccgagttctt
      301 ggcccggctc atcgaccacg tcgaccggct ggacgacgac gggcgatcct cgtgaacccc
      361 tggctctgga gctaccacgg ctacgagccg gacgaagagg gtgtgcgtga ggcgttgtgc
      421 gcgctcggca acggctacct cgccacccgc ggtgcgctac cggagtcaca cgccgacggc
      481 gcccactatc ccggcaccta catcgccggc gtgttcaacc gactgcgcgc cacggtcgag
      541 ggccagacgg tcgagaacga gagcttggtg aacgcaccga actggctggc gttcacgttg
      601 accggtccgg acggcaccac gttcgagtgc ggcacgaccg aggtgctcga ccacgagctc
      661 gagctcgacc tcaggcggag tctgctcacg cggcgcagtc gactgcgcga cgccaatgga
      721 cgcgtcctga gcatcaccca acgacggttc gtgagccttc gggactcgca cctcgccgcg
      781 ctcgagacga cgctcgtggc cgagaactgg tcgggccggc tcagggtcga gtcggcgctg
      841 gacggacggg tgacgaactc gggcgtgcga cgttacgccg cgctcggtac ggatcacctg
      901 gtcggccaag tcgctcggca gatcagcgac gacgcggtcg ccctcgacgt acagaccgtc
      961 gactcccacg tccgcatcgc aatggccgcc cgcaccaccg tcgacgtgcc caccgtgatg
     1021 gtcgatcgcg acgtcgtcac cgatgaccgc tacgtcgcac acgtcctcga tctcgagctg
     1081 caggaaggcg cgccggtggt cgtcgacaag atcgtcgcga tctacacctc gcgcgacgac
     1141 ggcatctacg agcccttgtc ggagtcggag gacgcggtgt gtcatgcccc atcgttcgac
     1201 gatctgctcg aacggcacgt agtgagttgg aaccacgcgt gggagcgggg aaggatcgac
     1261 atcaccgacg ggcagggtca caccggccgc gtcctcaacc tgcacatctt ccacatgttg
     1321 cagacgacgt cgaagaacac cgctgagctc gatgtcggtg ttccggcgcg tggcctgcac
     1381 ggcgaggcgt atcgcggtca catcttctgg gacgagctgt tcatcttccc gttcctcaac
     1441 tggcgcgtgc cggagctgag tcgcgccctg ctgcgctatc gctcccggcg gctcgaccgc
     1501 gcccgccgca acgcggccga ggccggttat cgcggcgcgc tgtatccctg gcagagcgcc
     1561 agcagtggcc gcgaagaaac ccagacgctg cacctcaacc ccaaatcggg ccggtggctc
     1621 ccggacgcat cgcacctgca gcaccacatc aacggcgccg ttgcctacaa cgtgtggcag
     1681 tactggcagg tgacggccga catcgagttc atgcggttct ggggcaccga gatgctcctc
     1741 gagatcgcgc ggttctggtc gagcatggcc acgtacaacc acacgctcga tcggtacgag
     1801 atcaaagggg tgatgggtcc cgacgagtat cacgaggcct acccccacgc ggacacaccc
     1861 gggttgaaca acaacgccta caccaacgtc ctcgcggtgt ggtgtctgat ccgcgcgttc
     1921 gacgcgctgg cgctccttcc ggagtcacgc cggcggcaac tgcgcgagaa gctcgtgctc
     1981 acgtcagagg agctcgatcg atggggcgac gtgagccgaa agatgcggct gtgcttccac
     2041 gacggtgtgc tcagccagtt cgagggatac gaccaactgg aagagctgga ctgggaggac
     2101 taccgggctc ggtacggcaa catccagcgg ttggatcgga tcctcgaatc ggaaggcttg
     2161 tcgaccaata actacaagct ggccaagcag gccgacacgc tgatgctctt ctatctcttc
     2221 tccgagaagg agctgagcca gctcatcgag cgcttgggtt acgagtaccc gccggacttc
     2281 ctgcggcggc acctcgagta ctacgagccg cgcacgtcac acggttcgac gctgagccag
     2341 accgtggcgt cgttcgtctt cagtcgcatc gatcgagagc gttcgtggga cctcttccgt
     2401 gaagcgctac agagcgatgt gaccgactcg caaggcggca cgacggccga gggcatccac
     2461 ctcggcgcga tggccggcac cgtcgacctc gtccagcgct gctacacggg gatcgagaca
     2521 cgcgacgaca tcctccgctt ggatcccgtg cttccggtgg agctgggttc gctttcgttc
     2581 gacgtgcggt acagggggca cgcgatgcac ctcgagttca ctgccgacta cgtcaggatc
     2641 aagtccgaac gggaaggcgc agccgtcgac gtgccgatgc ccgtggacgt gtgcggcacg
     2701 atctacgaga tcattccggg cgcgacgctc gaggtgaagc tgggctagtt ggtcgcggcc
     2761 tcaggcccgg ccgatcacct cgccgtgcag caccgcgaag aacccgtcgg gctcctcgct
     2821 ccaccggcgc caggcgtcgg cgatccgcgc gagttcgtct ttgtcggcca gcccgtagtc
     2881 gaccgcctgc tcggcgtatg acgacagcag cacccggtcg gcccacagat cgccccacca
     2941 gtggcgcgag tcaggatcgg cgaaggtcca cgtggagctg ccgacgtgct cgatctcgaa
     3001 ccctgcagcg cgcacccaac cgagtaggaa gcgcccggcg tcggcctcgg cacggttgcg
     3061 ttgcgtgatg ccgtggtaca cctcgttcca tcgctcgagg agcggatcgc gcggtgccca
     3121 cgtg
//
LOCUS       DASUGA010000859         2191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_802352, whole genome shotgun sequence.
ACCESSION   DASUGA010000859 DASUGA010000000
VERSION     DASUGA010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802352"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1199)
                     /locus_tag="VFX21_16945"
     CDS             complement(18..1199)
                     /locus_tag="VFX21_16945"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX5097714.1"
                     /translation="MTEPSRRRVAVTGLGVVATCGTGPDAFWSGLLAEPPVGERRIPS
                     FDPAEWFDNPKKARRTDRFAQFAFAAAAQALEQAGDISGDPTRKGVWIGTGVGGLITV
                     EDQILVRHEKGDRRVSPFLVPMMMPNAAAAGISMKYGWQGPCETTTTACAAGTHSVGN
                     GARLIQQGRCDAVIAGSSEAAMTPTGIAGFTNMTALSSSGISRPFDRNRDGFVMAEGG
                     AVLVLEEWEAAERRGATILAEVLGSASTADAHHITAPSPGGSGAVACMELALQDAGID
                     AAAVRHVNAHGTSTPLNDAAEAEAIVKVFGETGPPTTSIKGVTGHALGAAGALEAVAV
                     VLSMINGVIPPTAGYREPDPEMAAIDVVREPRPWEPGPTLSNSFGFGGHNGCVVFAPA
                     E"
     gene            complement(1199..1435)
                     /gene="acpP"
                     /locus_tag="VFX21_16950"
     CDS             complement(1199..1435)
                     /gene="acpP"
                     /locus_tag="VFX21_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX5097715.1"
                     /translation="MADEVFDRFQKCAVEVLAVDASQVVPDAKFGDDLDADSLDLVEL
                     VMALEEEFDISVDESELEGIETVKQALDLVQSKL"
     gene            complement(1468..2181)
                     /locus_tag="VFX21_16955"
     CDS             complement(1468..2181)
                     /locus_tag="VFX21_16955"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012183349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEX5097716.1"
                     /translation="MSRVALVTGGGRGIGLAIARRLLADGYRVAVTYRSSPSPEEPGM
                     LAVACDVTDTEQVDAAFDQIEAELGNVEVLVSNAGITADMLVLRMSDDDFARVIDTNL
                     AGALRVSRRAVQRMMRARWGRIILISSVVGATGQAGQANYAASKAGLVGLGRSLAREF
                     ASRNVTVNVVAPGPIATEMFEAVPDERRELIAGAVPLGRLGAAHEVAAAVAFLAGDDA
                     GYITGAVVPVDGGLGMGAW"
BASE COUNT          313 a          792 c          739 g          347 t
ORIGIN      
        1 gtccacgtcg agccggccta ctcggcgggg gcgaagacga cgcagccgtt gtggccgccg
       61 aagccgaagc tgttcgacag cgtgggtccg ggctcccacg ggcgcggttc gcgcacgacg
      121 tcgatggcgg ccatctcggg atcgggctcg cggtagccgg cggtcggggg gatcacgccg
      181 ttgatcatgc tcagcacgac ggcgaccgct tcgagcgcac cggccgcacc gagcgcgtgc
      241 ccggtgacgc ccttgatcga cgtcgtcggc ggtccggttt cgccgaagac cttgacgatg
      301 gcctcggcct cagccgcgtc gttcaacggc gtcgacgtgc cgtgcgcgtt gacgtggcga
      361 actgcggccg catcgatccc cgcgtcctgg agcgcgagct ccatgcacgc caccgcgccg
      421 ctgccaccgg gcgacggtgc ggtgatgtgg tgggcgtcgg cggtggatgc cgatccgagc
      481 acttcggcga ggatcgtcgc gccgcgtcgc tctgctgctt cccattcctc caacacgagc
      541 accgcgccac cctcggccat cacgaacccg tcgcggttgc gatcgaacgg gcgcgagatg
      601 cccgacgacg agagggcggt catgttcgtg aagccggcga ttccggtggg tgtcatggcc
      661 gcttcgctgc tgccggcgat caccgcgtcg caccgaccct gctggatcaa ccgcgcgccg
      721 ttgcccacgg agtgggtgcc cgccgcgcac gcggtggtcg tggtctcgca cggtccttgc
      781 cagccgtact tcatcgagat gcccgcggcc gcggcgttgg gcatcatcat cgggacgagg
      841 aacggcgata cgcgtcgatc gcccttctcg tgccgcacga ggatctggtc ttcgacggtg
      901 atgagcccgc ccacgcccgt cccgatccac actcccttgc gcgtggggtc gccactgatg
      961 tcgccggctt gttcgagcgc ctgcgcggcc gctgcgaacg cgaactgcgc gaaccgatcc
     1021 gtgcggcgcg cttttttcgg gttgtcgaac cattcggccg ggtcgaacga ggggatgcgt
     1081 cgctcgccga ccggtggctc ggcgaggagg ccagaccaga acgcgtccgg cccggtgccg
     1141 cacgtcgcca cgacgccgag cccggtgact gcgacgcgcc ggcgcgacgg ttcggtcatc
     1201 agagcttcga ctggacgaga tccagcgctt gcttgacggt ctcgatgccc tcgagctcgg
     1261 actcgtcgac gctgatgtcg aactcctcct cgagcgccat caccagctcg accaggtcga
     1321 gactgtcggc gtcgaggtcg tcgccgaact tggcgtcggg caccacctgg ctcgcatcga
     1381 cggccagcac ctcgaccgcg cacttctgga accggtcgaa gacttcgtca gccatgagct
     1441 gtttctcctt gtcgttgtca ccactgccta ccacgcaccc atcccgagcc cgccatcgac
     1501 cggcaccacc gcgccggtga tgtagccggc gtcgtcgccg gcgaggaagg cgaccgcggc
     1561 cgcgacctcg tgagctgcgc ccaacctgcc gagcgggacc gcgcccgcga tcagctcgcg
     1621 ccgctcgtcg ggcaccgcct cgaacatctc ggtggcgatc gggccgggcg cgaccacgtt
     1681 cacggtcacg ttgcggctcg cgaactcgcg tgccagcgaa cgtcccaacc cgacgaggcc
     1741 cgccttcgac gccgcgtagt tcgcctggcc agcttgaccg gtcgcgccca cgaccgagct
     1801 gatgaggatg atccgccccc agcgcgctcg catcatgcgt tgcaccgcgc gccgagacac
     1861 gcgcagcgcg ccggccaggt tggtgtcgat gactcgcgcg aagtcgtcgt cgctcatccg
     1921 cagcacgagc atgtcggccg tgatgccggc gttcgacacg agcacctcga cgttgccgag
     1981 ctccgcttcg atctggtcga acgccgcatc gacctgttcg gtgtcggtga catcgcacgc
     2041 gacggcgagc atgcccggtt cctccggaga aggagagctc cggtaggtga ccgccacgcg
     2101 gtagccgtcg gcgagcaagc ggcgcgcgat ggccagaccg atgccccgcc cgccgccggt
     2161 cacgagagcc acgcggctca tgtgcgcaca c
//
LOCUS       DASUGA010000860         4961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_591353, whole genome shotgun sequence.
ACCESSION   DASUGA010000860 DASUGA010000000
VERSION     DASUGA010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591353"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VFX21_16960"
     CDS             <1..814
                     /locus_tag="VFX21_16960"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018636796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX5097717.1"
                     /translation="IVGAHFVRDVEPGELVAIDASGVRSIRYADAHPKLCVFEFVYIA
                     RPDTQLYGHSVHAARQRMGEELARQAPCAADMVMPVPESGVPAAQGFARASGIPYGDG
                     FVKNRYVGRTFIQPSQKQRGAGVRVKLNPLRDNIRGKRVVVVDDSIIRGTTTRQTIAL
                     LREAGATEVHFRVSSPPYRWPCFYGLDTGQRSDLLAADMSVGEIADYLGVDSLAYLEL
                     DNLLTATGSAPEAFCTACFSGDYPVPVPDHDTKLALEVEKRTRHVSGTVEHA"
     gene            811..1863
                     /gene="purM"
                     /locus_tag="VFX21_16965"
     CDS             811..1863
                     /gene="purM"
                     /locus_tag="VFX21_16965"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020522555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX5097718.1"
                     /translation="MSNNSGAEKLTYAGAGVDIAAGEKAVELIKHHVRSTFRREVVGD
                     VGGFGGLFAPDWKQYKQPLLVSSTDGVGTKSLLARLANKRDTIGIDCVAMSVDDIAAQ
                     GAEPLFFLDYISIGKLVPEEIDELVSGVAEGCRQAHCALLGGEMSEHPGVMEPGEFDL
                     VGFAVGMVERDQVLPRNVRTGDRIIGLASPNLRSNGFSLARAALLERGGRDLFGPAWE
                     GAHHSLADELLAPSVIYAPAMLKLAREAKVHAFAHVTGGGIPGNIERVLPERCDALIR
                     RGTWDEPRIFGEIQRAGGVGDDEMEHVFNLGVGMLAVVPADRALHAVDTARAAGHDAW
                     LIGEIVDGHARAHLSR"
     gene            1809..2771
                     /locus_tag="VFX21_16970"
     CDS             1809..2771
                     /locus_tag="VFX21_16970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097719.1"
                     /translation="MVDRRDRRRARSRASVALALALAAALAVAGCAGDDDDGAAPATT
                     EEPPVTTTTTVPAAARLCAAAPPEQIGTITNPAIAELSGLAASRAFDGIYWAHNDSGD
                     RARIFAIQSDGATRAELSIDGAGAVDWEDIAVDESTIYVGDIGDNNSARANVTIYEVP
                     EPDVSIPDPVAVVGDAVRHDVTYEDGAHNAEALMVDPIAHELVIVTKELSGQSGIYTT
                     SQSAPDVFRRAGEVDLGIAQLATAGDISADGRVIVVRTYGNVFVWDRTEGESPAAAMA
                     REPCAAPAPSEQQGEAIALDPDGASFVTASEGAAPALFRVGPPR"
     gene            3217..3414
                     /locus_tag="VFX21_16975"
     CDS             3217..3414
                     /locus_tag="VFX21_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BldC family transcriptional regulator"
                     /protein_id="HEX5097720.1"
                     /translation="MQPHDDDALLTPSEVAAMFRVNPKTVTRWARAGKISAIRTLGGH
                     RRFRASEIRTFLEQVEDSERI"
     gene            complement(3501..3573)
                     /locus_tag="VFX21_16980"
     tRNA            complement(3501..3573)
                     /locus_tag="VFX21_16980"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3538..3540),aa:Phe,seq:gaa)
     gene            complement(3584..3658)
                     /locus_tag="VFX21_16985"
     tRNA            complement(3584..3658)
                     /locus_tag="VFX21_16985"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3621..3623),aa:Asp,seq:gtc)
     gene            complement(3672..3744)
                     /locus_tag="VFX21_16990"
     tRNA            complement(3672..3744)
                     /locus_tag="VFX21_16990"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3708..3710),aa:Glu,seq:ttc)
     gene            3824..4228
                     /locus_tag="VFX21_16995"
     CDS             3824..4228
                     /locus_tag="VFX21_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097721.1"
                     /translation="MRVAKFTKTLGGVAICAGLAACSGGGSGGPTALTASPEAACTLI
                     GRLSDTGELVEKADISDPEAFESALDDAVAKYVATIDDLRKVAPDDLRDDLDRLQAAV
                     EQYDFDDAFAARAPLDEYADAECTEDTTTTGS"
     gene            complement(4215..>4961)
                     /locus_tag="VFX21_17000"
     CDS             complement(4215..>4961)
                     /locus_tag="VFX21_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097722.1"
                     /translation="VPMDGSAFGPKHGGGEARDRGGRRGPRRGRRALVAGGALGVVVI
                     AAVIAIGASTLGEGTQPRTEELAAVEAPAPTASGTDATVTPTTAPSTAAAPTTGAPTP
                     TTARATPTTSRQPGGLGTTVAPTPTTAAPVATPTTAPPAPTTTLPPVTMRLSINPKSL
                     PQHYLVKDAPLLTWGVTNAATVHISGPKIDATTTSGSVRLCPGTPDANGFCNAPAGTY
                     PYELEAKSADGTILGTRTATLTITGSLTIP"
BASE COUNT          761 a         1697 c         1741 g          762 t
ORIGIN      
        1 catcgtcggc gcgcacttcg tccgcgatgt ggagccgggt gagctcgtcg cgatcgacgc
       61 gagcggcgtg cggtcgatcc ggtatgccga cgcgcacccc aagctgtgcg tgttcgagtt
      121 cgtctacatc gcgcgccccg acacgcagct gtacggccac agcgtgcatg ccgcgcggca
      181 gcggatgggc gaggagctcg cgcgccaggc gccgtgcgcg gccgacatgg tgatgcccgt
      241 gcctgaaagc ggcgtcccgg ccgcgcaggg ctttgcgcgc gcgtcgggca ttccgtacgg
      301 cgacgggttc gtgaagaacc gttacgtcgg gcgcacgttc atccaaccga gccagaagca
      361 gcgcggcgcg ggtgtgcgcg tgaagctcaa cccgctgcgc gacaacatcc gcggcaagcg
      421 cgtcgtcgtc gtcgacgact ccatcatccg cggcacgacg acgcgccaga cgatcgcgct
      481 gctgcgggag gcgggcgcca ccgaagtgca cttccgggtg tcgtcgccgc cgtaccgctg
      541 gccgtgcttc tacggcctcg acaccgggca gcgctccgac ctgctggccg ccgacatgtc
      601 ggtcggtgag atcgccgact acctcggcgt cgactcgctc gcatatctcg agctcgacaa
      661 cctgctcacg gccaccggtt ccgcgcccga agcgttctgc accgcgtgct tcagcggcga
      721 ctatccggtc ccggtccccg accacgacac gaagctcgcg ctcgaagtcg agaagcggac
      781 ccgccacgtg agcggtaccg tcgaacacgc gtgagcaaca actcgggcgc cgagaaactg
      841 acgtacgccg gcgccggcgt cgacatcgcg gccggcgaga aggccgtcga gttgatcaag
      901 caccacgtgc ggtcgacgtt ccgccgcgag gtcgtcggcg acgtcggcgg gttcggtggg
      961 ctgttcgcgc ccgactggaa gcagtacaag cagccgctgc tcgtgtcgtc caccgacggc
     1021 gtcggcacca agtcgctgct ggcgcgcctg gcgaacaagc gcgacacgat cggcatcgac
     1081 tgtgtcgcga tgtcggtcga cgacatcgcc gcccagggcg cggagcccct cttcttcctc
     1141 gactacatct cgatcggcaa gctcgtaccc gaggagatcg acgagctcgt ctccggcgtc
     1201 gcggagggct gccgccaggc gcactgcgcg ctgctcggcg gcgagatgtc ggagcatccg
     1261 ggcgtgatgg agcccggcga attcgatctc gtcgggttcg cggtcggcat ggtcgagcgc
     1321 gaccaagtgc tcccgcgcaa cgtgaggacc ggcgaccgca tcatcggcct tgcgagcccc
     1381 aacctccgct cgaacgggtt ctcgctggcg cgggcggcgc tgctcgaacg cggcggtcgc
     1441 gacctcttcg gccccgcgtg ggagggtgcg caccactcgc tcgccgacga actgctcgcg
     1501 ccgagtgtga tctacgcgcc cgcgatgttg aagctcgcgc gagaggcgaa ggtccacgcg
     1561 ttcgcgcacg tcacgggggg cgggattccc ggcaacatcg agcgcgtgct ccccgagcgc
     1621 tgcgacgcgt tgatccggcg cggcacctgg gacgagccgc gcatcttcgg cgagatccag
     1681 cgcgcgggcg gcgtcggcga cgacgagatg gagcacgtgt tcaacctcgg cgtcggcatg
     1741 ttggcggtgg tgcccgccga ccgggccttg cacgccgtcg acaccgcccg ggctgcgggc
     1801 cacgacgcat ggttgatcgg cgagatcgtc gacgggcacg ctcgcgcgca tctgtcgcgc
     1861 tagcgctggc actcgcggcc gcgctcgcgg tcgccggttg cgcgggcgac gacgatgacg
     1921 gcgcggcgcc cgcgacgacc gaggaaccgc ccgtcaccac cacgacgacg gtcccggccg
     1981 cggcgcgact gtgcgcggcg gcgccgcccg aacagatcgg cacgatcacc aaccccgcga
     2041 tcgccgagct gtcgggtctg gccgcgagtc gggcgttcga cggcatctac tgggcgcaca
     2101 acgactccgg tgaccgtgcg cgcatcttcg cgatccaaag cgacggtgcc actcgcgcgg
     2161 agctgagcat cgacggcgcg ggagccgtcg actgggaaga catcgccgtc gacgagtcga
     2221 cgatctacgt cggcgatatc ggcgacaaca acagcgcgcg ggcgaacgtc acgatctacg
     2281 aagtgcccga accggacgta tccattcccg atcccgttgc cgtcgtcggc gacgcggtgc
     2341 gccacgacgt cacctacgaa gacggcgcgc acaatgcaga ggcgctgatg gtcgatccga
     2401 tcgcgcacga gctggtgatc gtgaccaagg agctcagcgg ccagtcgggg atctacacga
     2461 cgtcgcagtc ggcccccgac gtgttccggc gcgcgggcga ggtcgacctc ggcatcgccc
     2521 agctcgcgac cgcgggcgac atcagcgccg acggccgcgt gatcgtcgtg cgtacctacg
     2581 gcaacgtctt cgtgtgggat cggaccgagg gcgagtcgcc ggccgcggcg atggcacggg
     2641 agccctgcgc ggcgcctgca ccgagcgagc agcagggcga agcgatcgcg ctcgaccccg
     2701 acggcgcgag cttcgtgacc gcgtcggagg gcgcggcgcc cgctctgttc cgagttggcc
     2761 ctcctcggta ggccgcgact ttccctcttt ggtacaactc cggcagacct tacgtctggc
     2821 aggacgagaa aattgtcggc cttgaggagc gccctccggg gtcccgagat ctcgtacggc
     2881 cgcgtatctt atacgtattt acacggtgtg gcggaagtgt cctattatgg ggtagtgcgg
     2941 ctcgcccgcc gcgacaaggg aatgccttct ccacggggtt ctgcgaccag agtgaatcgg
     3001 tgagaagcct catcgagacg caattggtgg ccaaccgcgt cggcgttcgg ctccgttgcc
     3061 ggggtgagcg atccgctcat aggctgagtc atctggccca gtgggacggg atggcaatga
     3121 tcggcggatc cggacgtggg gtacggaacc gtttgaagcc atctcgcgat ggctcagcca
     3181 gacgattgcc cctcacacga cctcaaggtg gagacaatgc aaccccacga cgacgacgcc
     3241 cttctcactc cctccgaagt tgcagcgatg ttccgcgtca acccgaagac ggtgacccgc
     3301 tgggctcgcg ccggaaagat ctcggccatc cgcaccctcg gcggccaccg ccgcttccgc
     3361 gccagcgaga tccgcacgtt cctggaacaa gtggaagaca gcgaacgaat ctgatcggac
     3421 cgtcgcgcgg caaggggcga cgatctgggt caagaacaac caaagcgcgg cgaccaaccc
     3481 ggtcgccgcg tttttgcgtg tggccgggac cggcgtcgat ccggtgacct cgcgcttttc
     3541 aggcgcgcgc tctgccaact gagctacccg gccgggattt gcgcggtcct gacgggattt
     3601 gaacccgcgg cctccgcctt gacagggcgg cgtgcactcc aagctgcacc acaggaccag
     3661 attttcttac gcacccccaa ggggattcga acccctgtta ccgccgtgaa agggcggcgt
     3721 cctaggccac tagacgatgg gggctcgttc tcccagggga gacgaacact ctacatcttc
     3781 gccaaatgcc cgccatcagc gaacgcatcg ctaccttgac ctcgtgcggg tcgcgaagtt
     3841 cacgaagacg ctgggtggcg tcgcgatctg cgccggtttg gccgcgtgct cgggaggtgg
     3901 gagcggcggc ccgacggcgc tgacggcatc accggaggcc gcgtgcacgc tcatcggccg
     3961 cctgtccgac acgggggagc tggtcgagaa ggccgacatc agcgatcccg aggctttcga
     4021 gtctgcgctc gacgacgcgg tcgcgaagta cgtcgcgacg atcgacgatc tgcgcaaggt
     4081 cgcgcccgat gacctgcgcg acgatctcga ccggctgcag gccgcggtcg agcagtacga
     4141 cttcgacgac gcattcgccg cgcgcgcgcc cctcgacgag tacgccgatg ccgagtgcac
     4201 cgaggacacg acgactacgg gatcgtgagc gaaccggtga tcgtcagcgt tgcggtgcgg
     4261 gtgccgagga tcgtgccgtc cgcgctcttc gcctcgagct cgtagggata ggtcccggcc
     4321 ggtgcgttgc agaacccgtt ggcgtcgggc gtcccggggc acagccggac ggaaccggac
     4381 gtcgtggtcg cgtcgatctt cggtcccgag atgtgcacgg tggctgcgtt cgtcacgccc
     4441 cacgtcagca gcggcgcgtc cttcaccagg tagtgctgcg gcaatgactt cgggttgatc
     4501 gagaggcgca tcgtgaccgg cggcagcgtg gtcgtgggcg cgggcggcgc ggtcgtcggt
     4561 gtcgcaaccg gcgccgcggt cgtgggtgtg ggcgcgacgg tcgtaccgag tccgcccggc
     4621 tgacgcgatg tcgtcggtgt cgcccgcgcg gtcgtcggag ttggcgcgcc ggtggtcggc
     4681 gcggctgccg tcgacggtgc cgtggtgggc gtgacggtcg cgtcggtgcc ggatgcggtc
     4741 ggcgccggtg cttcgaccgc ggcgagctcc tcggttcggg gttgcgttcc ctcgccgagc
     4801 gtcgacgcac cgatcgcgat caccgccgcg atgacgacga cgccgagcgc gcctcccgcg
     4861 acgagcgcgc gccgccctcg gcgcggcccg cggcgtccgc cgcggtcgcg tgcttcgccg
     4921 ccaccgtgct tcggcccgaa cgcggagccg tccatcggga c
//
LOCUS       DASUGA010000861         3414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1080547, whole genome shotgun sequence.
ACCESSION   DASUGA010000861 DASUGA010000000
VERSION     DASUGA010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080547"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VFX21_17005"
     CDS             <1..849
                     /locus_tag="VFX21_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5097723.1"
                     /translation="RAGDDLVMGDRFAGGIQPGAMPALHKYVGNPLLSFAGRHMFGIP
                     VRDFHCGLRAARTDALRELGLVTTGMEFASEMVVRSALAELKISEVPTVLRPDGRDRP
                     PHLRTWRDGWRHLRYLLLFSPTWLFLIPGIVLAVIGLTATLALSIHGAISFGNVHFQV
                     STLVYAAALTVIGVQTATFALFTRIYAVAKGFLPATDRLDEFGGRFSLERGLIVGVVL
                     LLLGVAGAIISFVRWQQQDFGSLDFELQLRIVVPSALALVLGSWAILSSFLASILGFD
                     KTIKLG"
     gene            857..2620
                     /locus_tag="VFX21_17010"
     CDS             857..2620
                     /locus_tag="VFX21_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097724.1"
                     /translation="MTAVEAPTGTREEEHSRPDFERIVTTALLFASAMLFVMFCVRVL
                     GDGWTTHFPKVYPDSYSFTTVANRGPLHPSFFWDERAIGYPLFLWVTGRSTALNALGL
                     TLIYCAAYATLVVACLKAFTHRVAQAVAIFLILLTAVQLRFAQWSVQVLSETLALSTG
                     VFMVAAWLVFVATPTTRRLKIAFAATVVWMLVRDAHVLPTALVLVPIVLVAGLLPTTP
                     RQFRRLLLIGTAVLLGTCGYVYFSQSHSNRNITSFYDAVGGRILPDADLTKFFVDGGM
                     PMNDALRSRTNHVAWDDGPQSFLDNPDLAEFRQWARTEGNRRLLMAMVVKSPHFLEVM
                     GAEYPRVLNDDLTDYDSYHVSQRLPHRPLGDLSGPDSALSLRVWTFLALLGIAAAGSL
                     GFGARRERKEVTAAIVGAAVFLGVLSEYHLRGTQRVCFVVAVLLAASAYFLSAQRDRS
                     ASRVVLAAAFGLPLVGVFMPDNLRLLVMFGPPLLAAIFFVTARGRVEWRGLRFALFGI
                     AGIVLAFTEVYISYVGDSLEISRHAIGAIHRLAIMYPLCIAIGIDAAVRIFSSPQIVD
                     EEVEGDQAEEPEPEPLLEEAP"
     gene            2617..>3414
                     /locus_tag="VFX21_17015"
     CDS             2617..>3414
                     /locus_tag="VFX21_17015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097725.1"
                     /translation="MTDTLTDGPEAPVVEPPAAEPEARTEPLISPETRAAWRHGFPKF
                     VRWLTAGVFGTLVVISAIGNELRVQDADPMFMRVVVERVHAFGGAYYENAIHNKGPLE
                     PFVYSTALHLGGSEGMWYFISIFMTIAALVLAWSVAKAAAFTGGNRDVALAVAAVAFI
                     HMTLSPADYAGVLYARNMTIALLAIAFVAVFEPRAWRTPRGRVISSVIAGVTLGIAVQ
                     TLTPEVFAGAVIATGGLLLLPRRAAGTELRRLRLIFIGSGALAFLSAP"
BASE COUNT          454 a         1189 c         1129 g          642 t
ORIGIN      
        1 cgcgcgggtg acgacctcgt catgggcgac cgtttcgcgg gcggcatcca accgggtgcg
       61 atgccggcac tgcacaagta cgtcggtaac ccgctgttgt ccttcgccgg tcgccacatg
      121 ttcggcatcc cggtacgcga cttccactgc ggtctgcgcg ccgcgcgcac cgacgcgttg
      181 cgcgagctcg gcctcgtcac gacgggcatg gagttcgcga gcgagatggt cgtccggtcc
      241 gcgctcgcgg agctgaagat cagcgaagtc ccgacggtgc tgcggcccga cgggcgcgat
      301 cgcccaccgc acctgcgcac ctggcgcgac gggtggcggc acctgcggta cctgctgttg
      361 ttcagtccga cgtggctctt cctcatcccg ggcatcgtcc tggccgtcat cggcctcacc
      421 gccacgttgg cgttgagcat ccacggcgcg atctcgttcg gcaatgtgca cttccaggtc
      481 agcacgctgg tctacgcggc cgcgctgacg gtcatcggtg tgcagacggc gacgttcgcg
      541 ctgttcacgc gcatctacgc ggtcgccaaa gggtttctcc ccgcgaccga ccgactcgac
      601 gagttcggcg gccggttcag cttggagcga ggcttgatcg tcggtgtcgt gctcctgctc
      661 ctcggtgtcg cgggcgcgat catctccttc gtgcgctggc agcaacaaga cttcgggtcg
      721 ctcgacttcg agctccagct gcggatcgtc gtcccttcgg cgctcgcact cgtgctcggt
      781 tcgtgggcga tcctgtcgag cttccttgcg tcgattctcg gcttcgacaa gacgatcaag
      841 ctcgggtgac acgacgatga cggccgtcga agcgcccacc ggcacccgcg aggaagaaca
      901 ctcccggccc gacttcgaac gtatcgtcac cacggcgttg ctcttcgcgt cggcgatgtt
      961 gttcgtgatg ttctgcgtgc gggtgctcgg tgacgggtgg acgacgcact tccctaaggt
     1021 gtaccctgac agctactcgt tcacgacggt cgcgaaccgg ggcccgctgc acccgagctt
     1081 cttctgggac gaacgcgcga tcggctatcc gctcttcttg tgggtgacgg gccgcagcac
     1141 cgccttgaac gcgctcgggc tgacactcat ctactgcgct gcatacgcca cgctggtcgt
     1201 cgcgtgcctc aaggcgttca cgcaccgggt cgcgcaagcc gtcgcgatct tcctgatctt
     1261 gctgaccgcc gtgcagttgc gcttcgcgca atggtccgta caagtcctga gcgaaacgct
     1321 cgccctgtcg accggggtgt tcatggtcgc cgcgtggctc gtcttcgtag cgacacccac
     1381 tacgaggcgc ctcaagatcg cattcgcggc gacggtcgtc tggatgctcg tccgggacgc
     1441 ccacgtgttg ccgaccgcgc tcgtgctcgt gccgatcgtc ctcgtcgccg gtctgttgcc
     1501 cacgaccccg cggcagttcc ggcggctgct gctcatcggg acggccgtcc tgctcggcac
     1561 gtgcggttac gtgtacttct cgcagagcca ctcgaaccgc aacatcacct cgttctacga
     1621 cgcggtgggc gggcgcatcc tgccggacgc cgacctgacg aagttcttcg tcgacggcgg
     1681 gatgccgatg aacgacgcgt tgcgctcgcg gacgaaccac gtcgcgtggg acgacggccc
     1741 gcagtcgttc ctcgacaacc ccgacctcgc cgagttccgc cagtgggcgc gcaccgaagg
     1801 caaccggcgc ctgctcatgg cgatggtcgt gaagtcgccg cacttcctcg aagtgatggg
     1861 cgcggaatac ccgcgcgttc tcaacgacga tctcaccgac tacgactcgt atcacgtctc
     1921 gcagcgactc ccgcaccggc cgctcggcga cctgagcggg cccgacagcg cgctgtcgct
     1981 tcgcgtgtgg acgttcctcg cgctgctcgg catcgcggcc gccggcagcc tcgggttcgg
     2041 cgcgcggcgc gagcgcaagg aggtgacggc cgcgatcgtc ggtgcggccg tgttcctcgg
     2101 cgtgttgagc gagtaccacc tgcgcggcac acaacgggtg tgcttcgtcg tcgccgtgct
     2161 gctggccgcg tccgcctact tcctgtcggc gcaacgcgac cggtcggcga gtcgcgtcgt
     2221 gctcgccgcc gcgttcggtc tgccgctcgt cggcgtgttc atgcccgaca acctgcgtct
     2281 gctcgtcatg ttcgggccgc cgctgctcgc agcgatcttc ttcgtgacgg cgagagggcg
     2341 cgtcgagtgg cgtggcctgc gcttcgcgct gttcggcatc gccggcatcg tgctcgcgtt
     2401 caccgaggtc tacatctcgt acgtcggcga ctcactcgag atctcgcgcc acgcgatcgg
     2461 cgcgatccat cgcctcgcga tcatgtaccc gttgtgcatc gcgatcggga tcgacgcggc
     2521 cgtgcgcatc ttctcgtcgc cgcagatcgt cgacgaagaa gtcgaaggcg atcaagcaga
     2581 ggagcccgaa ccggagccgt tgctcgagga agcaccgtga cggacaccct gaccgacggt
     2641 cccgaggccc cagtggtcga gccgcccgcg gccgagcccg aggcgcgcac cgagcctctg
     2701 atctcacccg agacgcgcgc ggcgtggcgg cacgggttcc cgaagttcgt gcgctggctg
     2761 acggcgggcg tcttcgggac gctggtcgtg atctcggcca tcggcaacga gctgcgcgtc
     2821 caggacgccg acccgatgtt catgcgcgtc gtcgtcgagc gggtgcacgc gttcggcggt
     2881 gcgtactacg agaacgcgat ccacaacaag ggcccgctcg agccgttcgt ctactcgacc
     2941 gcgctgcacc tcggcggctc cgagggcatg tggtacttca tctcgatctt catgacgatc
     3001 gcggccctcg tgctcgcgtg gagtgtcgcg aaggccgcgg cgttcaccgg tggcaaccgc
     3061 gatgttgcgc tcgcggtggc cgcggtcgcg ttcatccaca tgaccctctc gcccgccgac
     3121 tacgcgggcg tgctctacgc gcgcaacatg acgattgcgc tgttggcgat cgcgttcgtc
     3181 gcggtcttcg agcctcgggc gtggcgcacg ccacgcgggc gcgtcatcag ctcggtgatc
     3241 gccggtgtga cgctcggcat cgcggtccag acgctgacac ccgaggtctt cgccggtgcg
     3301 gtgatcgcca ccggcgggtt gttgttgctc ccgcgccggg cggcggggac cgagctgcgc
     3361 cggctgcgct tgatcttcat cggcagcggc gcgctcgcgt tcctgagcgc accg
//
LOCUS       DASUGA010000862         2217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_385782, whole genome shotgun sequence.
ACCESSION   DASUGA010000862 DASUGA010000000
VERSION     DASUGA010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385782"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VFX21_17020"
     CDS             complement(<1..547)
                     /locus_tag="VFX21_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019739153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5097726.1"
                     /translation="MTDEVLVDIADRVALITLNRPDQRNALNRAIRKQLPTAITQLDA
                     DDDVDVMILTGADPAFSGGVDLKEFGSGAATPPADEPNRVDMGERADGRLPFRGALPP
                     HTKPLIGAINGVAVTGGLEIALNCDFLVASERARFADTHARVGVMPGWGLTVLLPERV
                     GVTRATEMSLTGNYVDAPTALA"
     gene            complement(551..1786)
                     /locus_tag="VFX21_17025"
     CDS             complement(551..1786)
                     /locus_tag="VFX21_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5097727.1"
                     /translation="MQALLYGVRPDPVPEPDTDNHLLVGLARTPMRLTEMVDPGFLLP
                     DWVVTRPRLVGICGSDAKQVFMDWGDVRTPDNPMKGFFSLPQVLGHEVVADVVALGPA
                     AEGLAVGDRVVLNPWLSCGPRGVSPICDACTRGDYSLCASFGVGPIAPGIHTGTSKDA
                     SGGYAELMPAHDSMLFKVPDAVADEDAVFADPFAVMLHGITRHAPPPGGKVMIYGAGA
                     LGTCATAIVRALWPDVDVLVVARFEAQAEMARRLGATVIGHEPALAVIEDAAAWSGGV
                     LQPSDGLPMAYPGGIDVVYDTVGKQETFEVGTRVLKARGTLVKAGVHGPTFWEDTPLY
                     FKEISMVGSNAFGVEEVDGVRKHGIEHYTDLVAGGRVDLTGMLTHTFRLPEWREAFTA
                     LATQDESGAIKVGFDFREN"
     gene            1876..>2217
                     /locus_tag="VFX21_17030"
     CDS             1876..>2217
                     /locus_tag="VFX21_17030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097728.1"
                     /translation="MAERRTQAEIQRQTRHVFMVGGGVVFFAVGAALILGAFLQDTSG
                     QLSSDDPVSTQAVATTTAVTTVAPANDAAPRSLALVATGDLLAQGLVNEDAAENGERS
                     GARYDFRPMFAE"
BASE COUNT          348 a          789 c          743 g          337 t
ORIGIN      
        1 aggcgagcgc ggtcggcgcg tcgacgtagt tgccggtgag actcatctcc gtcgcgcgcg
       61 tgacgccgac gcgttctggc aacagcacgg tgagacccca cccgggcatc acaccgacgc
      121 gcgcgtgcgt gtccgcaaaa cgggcacgtt cggacgcgac gaggaagtcg cagttcaacg
      181 cgatctccaa gccaccggtg acggccactc cgttgatcgc accgatgagc ggcttcgtgt
      241 ggggtggcag cgcgccgcgg aacggcagcc ggccgtcggc ccgctccccc atgtcgacgc
      301 ggttcggttc gtccgcgggc ggcgtcgccg cgcccgaacc gaactccttg aggtcgacgc
      361 cgcccgagaa cgcgggatcg gcgccggtca ggatcatcac gtcgacgtcg tcgtccgcgt
      421 cgagctgcgt gatcgccgtc ggcagctgct tgcggattgc ccggttgagc gcgttgcgtt
      481 ggtcgggacg gttgagcgtg atgagcgcga cccgatccgc gatgtcgacg agcacttcgt
      541 ccgtcactgt tcagttctcc cggaagtcga agccgacctt gatggcgccg ctctcgtcct
      601 gcgtcgccag cgcggtgaac gcctcgcgcc actccggcag gcggaacgtg tgcgtgagca
      661 tcccggtgag gtcgacccga ccgccggcga cgaggtccgt gtagtgctcg atgccgtgct
      721 tacggacgcc gtcgacctcc tcgacgccga acgcgttcga gcccaccatc gagatctcct
      781 tgaaatacag cggcgtgtcc tcccagaacg tcggcccgtg cacgccggcc ttcacgagcg
      841 tgccgcgcgc cttgagcacg cgggtgccga cctcgaacgt ctcctgcttc cccaccgtgt
      901 cgtacacgac gtcgatgcca cccggatacg ccatcggcaa tccgtcgctc ggctgcagca
      961 cgccacccga ccacgcggcc gcgtcctcga tcacggccag cgccggctcg tgaccgatca
     1021 ccgtcgcacc cagccggcgc gccatctcgg cttgcgcctc gaaccgcgca accaccagga
     1081 cgtcgacgtc gggccacagc gcgcgcacga ttgccgtcgc gcacgtgccg agcgcaccgg
     1141 cgccgtagat catcaccttg ccgcccggcg gcggtgcatg gcgcgtgatg ccgtgcaaca
     1201 tcaccgcgaa cggatcggcg aacaccgcgt cttcgtccgc gaccgcgtcg ggcaccttga
     1261 acagcatcga gtcgtgggcg ggcatcagct cggcgtagcc accgctggca tccttcgacg
     1321 tgcccgtgtg gatgccgggt gcgatcggtc cgacgccgaa cgacgcgcac aagctgtagt
     1381 cgccgcgggt gcacgcgtca cagatcggtg acacaccacg cggcccgcac gacaaccacg
     1441 ggttcagcac cacccggtct ccgactgcga gcccctcggc cgccggtccg agcgcgacga
     1501 catcggcgac gacctcgtgg ccgagcacct gcggcagcga gaagaagccc ttcatcggat
     1561 tgtcgggtgt gcgcacgtca ccccagtcca tgaacacctg cttcgcgtcg gaaccgcaga
     1621 tgccgaccag ccgcgggcgg gtgacgaccc agtccggcaa caggaacccc ggatcgacca
     1681 tctcggtgag ccgcatcggc gtgcgcgcca gcccgaccag gaggtggttg tcggtgtcgg
     1741 gttcgggcac cggatcaggg cgaacgccgt agagcagtgc ttgcatgaaa cgggaccgta
     1801 actcgcgggc gaggatccgt gcgccggacc ctttgcatcc atgcgacgga ctggtcggcg
     1861 acgtatcttc cgcgcgtggc ggaacggagg acgcaggccg agatccagcg gcagactcgg
     1921 cacgttttca tggtgggcgg aggcgtcgtc ttcttcgcgg tgggcgcggc attgattctc
     1981 ggcgcgttcc tccaggacac gagcggccaa ctctcctccg acgatcccgt gtccacgcaa
     2041 gcagtcgcga ccacgaccgc cgtgacgacg gtcgcgccgg cgaacgacgc cgcgccgcgt
     2101 tcgttggcac tggtcgcgac gggcgacctg ctcgcccagg gcctggtcaa cgaggacgcg
     2161 gccgagaacg gtgagcggtc cggtgcgcgg tacgactttc gcccgatgtt cgcggag
//
LOCUS       DASUGA010000863         3011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1776417, whole genome shotgun sequence.
ACCESSION   DASUGA010000863 DASUGA010000000
VERSION     DASUGA010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776417"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..752
                     /locus_tag="VFX21_17035"
     CDS             63..752
                     /locus_tag="VFX21_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018218612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX5097729.1"
                     /translation="MAQVEAVVVGCRRCPRLVAWREEVARTRRAAFADQEYWGRPVPG
                     FGDPRARVLVVGLAPAAHGGNRTGRVFTGDRSGDFLFASMYRTGFANQALSTGLDDGL
                     RLRDCYIAAAVRCAPPANKPTPLERDTCRPFLVREIGLLTRLRVVVVLGSFGYEAAWT
                     SLRDAGFAMPSRRPRFGHGIEVDCGRVTIIGSYHPSQQNTFTGKLTEAMLDAVFARAQ
                     SLTMATSSPTA"
     gene            complement(713..1492)
                     /locus_tag="VFX21_17040"
     CDS             complement(713..1492)
                     /locus_tag="VFX21_17040"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5097730.1"
                     /translation="MPDWLQVVLTLLVGVATGVLSGMFGIGGAVVSTPAVRALGATPL
                     EAVGSTLPSIIPSSISGTLRYHRDGFVRWRVVAWTASFGVVASVGGALASDVFPGGGH
                     VLMLLTAALVAFTAFRTAFPPVRIERDLANMHIDWWRLALIGIAAGGLSGLLGIGGGI
                     LMVPAFSAWLGLPLKDTIATSLACVGMLAIPGTITHAFLGHIDWAIAIPLSIGVIPGA
                     RIGAHVTINTGDRRLRYTVGAVLGILALVYAVGELVAIVSD"
     gene            1575..2471
                     /gene="dapA"
                     /locus_tag="VFX21_17045"
     CDS             1575..2471
                     /gene="dapA"
                     /locus_tag="VFX21_17045"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX5097731.1"
                     /translation="MSELDLRGVWIPLITPFDERDEVDVAAIERLCREYLAAGAVGIV
                     ALGTTGEAAALDAAEKQVVVDACSRVCVDAHAPLIVGTGSYNTKQTIAATQALANVPA
                     CVGALVVTPYYVRPSEEGIVAHFKAVAAASPVPLVLYNIPARTGRAISASAMLELAAV
                     PNIAGVKHAYASLDADTLHLLANAPAQFQVLGGEDTLLFPLMCMGSVGTICASAHLLT
                     ERFVAMIECGLNGKTEDGRAHAEALLPVVQAIFAEPNPALLKAALHAQGRIATPNLRL
                     PMTNASSGALDRLLSAVERASH"
     gene            2569..2775
                     /locus_tag="VFX21_17050"
     CDS             2569..2775
                     /locus_tag="VFX21_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097732.1"
                     /translation="MSRKVAWMLVALCAWTLYVWITRLWIMAGQDESTAFKVVHGILA
                     VVSIAFGVFAGWVGVRRLRTGDPG"
     gene            2822..>3011
                     /locus_tag="VFX21_17055"
     CDS             2822..>3011
                     /locus_tag="VFX21_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5097733.1"
                     /translation="MSAAPPAVPLVPFDRESRHYHQRWWILGVLCLSLLVIIVDNSIL
                     NVAIPTLVKDLDASNSQLQ"
BASE COUNT          459 a         1036 c         1076 g          440 t
ORIGIN      
        1 gcgatcgcgg gcgaggaagc cgagcggcgt ccgtgacgga ggctccgcct ccgttcgcgt
       61 ccatcgcgca ggtcgaggcg gtggtggtgg gctgccgcag gtgtccgcgc ctcgtcgcct
      121 ggcgcgagga ggtcgcgcgc acgcgtcgcg ccgcgttcgc cgaccaggag tactggggtc
      181 gaccggtccc cggcttcggc gacccgcgcg cacgggtcct cgtcgtcggc ctcgcgcccg
      241 cggcccacgg cggcaatcgc accgggcgcg tgttcacggg cgatcgctcg ggcgacttcc
      301 tgttcgcgtc gatgtaccgc accggcttcg cgaaccaggc gttgtcgacc ggactcgacg
      361 acggcttgcg actgcgcgac tgctatatcg ccgccgcggt ccggtgcgcg ccgccggcca
      421 acaagccgac acccctcgag cgcgacacgt gccgcccatt cctcgtgcgc gagatcggtt
      481 tgttgacgcg gctgcgcgtc gtcgtcgtgc tcgggtcgtt cgggtacgag gcggcgtgga
      541 cgtcactgcg cgacgccggc ttcgcgatgc cgtcgcggcg gccgcgcttc ggtcacggca
      601 tcgaggtcga ctgcggccgg gtcacgatca tcggctcgta tcacccgagc cagcagaaca
      661 cgttcacggg caagctcacc gaggcgatgc tcgacgcggt gttcgcacgc gctcagtcgc
      721 tgacgatggc cacgagctcg ccgaccgcgt agacgagcgc gaggatgccg agcaccgcgc
      781 cgacggtgta gcgcaggcgc cggtcgccgg tgttgatcgt gacgtgtgca ccgatgcgcg
      841 cgcccgggat gacaccgatg ctgagcggga tcgcgatggc ccagtcgatg tgccccagga
      901 atgcgtgcgt gatcgtgccc gggatcgcca gcatgccgac gcacgcgagc gacgtcgcga
      961 tcgtgtcctt gagcggcagc ccgagccagg cactgaacgc gggcaccatg aggatcccgc
     1021 cgccgatgcc gaggaggccc gacaggccac cggcggcgat gccgatgagc gccagtcgcc
     1081 accagtcgat gtgcatgttc gcaaggtcgc gttcgatgcg gacgggcggg aacgcggtgc
     1141 gaaaggccgt gaacgcgacc aatgcagcgg tcaacagcat gaggacgtgc ccgccgcccg
     1201 ggaacacgtc ggatgccagc gcgccgccga ccgaggcgac gacgccgaac gacgccgtcc
     1261 acgccacgac ccgccagcgc acgaagccgt cgcggtggta gcgcagcgtc cccgagatcg
     1321 acgacgggat gatcgacggc agggtcgagc ccaccgcttc gagcggcgtc gcgccgagtg
     1381 cacgaaccgc cggggtcgac acgaccgcgc cgccgatgcc gaacatcccc gacagcacac
     1441 ccgtcgcgac gccgacgagc aacgtgagga caacctgcag ccaatccggc acggcggccg
     1501 aagctagtcg ccgcctagac ttggacgtgc ggacgcaacg aagccacgga cggcgcagtg
     1561 agggagtcct gggaatgagt gagctcgacc tgcggggggt ctggatcccg ttgatcacgc
     1621 cgttcgacga gcgcgatgag gtcgacgtcg ccgccatcga acggttgtgc cgggagtacc
     1681 tggcggccgg tgcggtgggc atcgtcgccc tcggcaccac cggtgaagcg gcggcgctcg
     1741 acgcggcgga gaagcaggtc gtcgtcgacg cgtgctcgcg tgtgtgcgtc gacgcgcacg
     1801 cgccgctgat cgtcggcacg ggttcgtaca acacgaagca gacgatcgcg gcgacgcagg
     1861 ccctcgcgaa cgtgccggcg tgcgtcggcg cgctcgtcgt cacgccgtac tacgtgcgtc
     1921 cgtccgagga gggcatcgtc gctcacttca aggccgtcgc ggccgcgagc ccggtgccgc
     1981 tggtgctcta caacattccg gcgcgcacgg gtcgtgcgat cagcgcgtcg gcgatgctcg
     2041 agctggccgc ggtgccgaac atcgccggcg tgaagcacgc atacgcgtcg ctcgacgccg
     2101 acacgctgca cttgctcgcg aacgcaccgg cgcagttcca ggtcctcggc ggcgaggaca
     2161 cgctgctgtt cccgctcatg tgcatgggct cggtcggcac gatctgcgcg tcggcgcacc
     2221 tcctgaccga gcgcttcgtc gcgatgatcg agtgcggcct caacgggaag accgaagacg
     2281 gtcgcgcgca cgccgaggcg ctgttgccgg tggtgcaggc gatcttcgcg gagccgaacc
     2341 cggcgctgct caaggctgcg ctgcatgccc agggccgcat cgcgacgccg aacctgcgcc
     2401 tgccgatgac gaatgcgtcg tcgggtgctc tcgaccgcct gctttcggcc gtcgaacgcg
     2461 cctcgcacta gaacggagcg cgtgaggcga ggatctgagg cagcgactcg gacgcgcgag
     2521 ggtccgagcc ggggcccgag cggcccggcg cagccgagag cggaagtgat gagtcgaaaa
     2581 gtcgcgtgga tgttggtcgc gctgtgcgcg tggaccctct acgtctggat cacccggttg
     2641 tggatcatgg ccggccagga cgagagcacc gccttcaagg tcgtgcacgg gatcctggcg
     2701 gtcgtctcga tcgcgttcgg cgtgttcgcg ggctgggtcg gcgtgcggcg gctgcggacc
     2761 ggtgatcccg gctagaccgg gtgttgcgcc gaaacgaaac cgtacagtat cgtaatcggc
     2821 catgtcagcc gcgccgcccg ccgtgcccct cgtccccttc gaccgcgaga gccgccacta
     2881 tcaccagcgc tggtggatcc tcggcgtcct gtgcctctcg ctgctggtca tcatcgtcga
     2941 caactcgatc ctgaacgtcg ccatcccgac gctcgtcaag gacctcgacg cgtcgaacag
     3001 ccagctgcag t
//
LOCUS       DASUGA010000864         2802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_2018241, whole genome shotgun sequence.
ACCESSION   DASUGA010000864 DASUGA010000000
VERSION     DASUGA010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2018241"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="VFX21_17060"
     CDS             <1..1215
                     /locus_tag="VFX21_17060"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX5097734.1"
                     /translation="GSTGVENSYSGDLSGRTFTLNINNLEDVFANRQIVGTVVLTLSK
                     QIQQAAAGALGDHPGSVVALDIKTGAVLAMYSNPTFDPNPLVSHDTAEAQRAFALDQL
                     NPGKPLLPRAWREIFPPGSTFKTVTAAIAQDTNTDVDKVFPDVRFIPLPQTTAILRNF
                     GDEQCGGTMEESFIVSCNTTFGQIGLDLGEKFATGIKNFGVQTDPPRQSGSGIDPPIV
                     KSLGPPEGSFQQNQPAFAQAAIGQNATAVTPLQMAMVAESVATGGTILQPHVVDCVLD
                     PNGDPVKKVPVQEYKQAMTAATAETMRTFMLGVVNDPNGTGSAARIPGVLVAGKTGTA
                     QTGEGQRNHAWFITFAPADQPRYAVAVLVEHGGANTEATGGRVAAPIARQVLEALLAS
                     QLPPSRCGSGQP"
     gene            1249..>2802
                     /gene="pknB"
                     /locus_tag="VFX21_17065"
     CDS             1249..>2802
                     /gene="pknB"
                     /locus_tag="VFX21_17065"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEX5097735.1"
                     /translation="MTLAGRVFSNRYEIQREIAQGGMAEVYLARDQLLNRPVALKALF
                     PEYAREPSFVERFRREAQAAANLNDPNIVAIYDWGQESGTYFIVMEYVEGRSLRDLIR
                     AEAPVDPNQAAEITAEIASALAYAHRNGVVHRDVKPGNVLLTRSGNVKVTDFGIARAG
                     ASDGLTQTGSVMGTATYFSPEQAQGLPVDGRSDVYALGVVLYEMVTGVAPFTGDSPVS
                     VAYKHVREDVVPPSQRNAEIPPELEQVIMTALAKDPNQRYQTADDLRADLLRFRRGRP
                     VTGAVTAIVTEVPTTATAAAAGAYAATMATPVVDDRGRATTGAQYPAKKKNTALIWVL
                     TLLALALVIGGILFAATQLGDSQEQVSVPNVEGKPIDEAQSILDDAHLKYDITNVNTP
                     LVKAGTVVEQTPKEGASLARGSSVKLTVSAGAEEKAVPTDLVNHPFAEVSKKLTDAGF
                     TVKRTDRPHDTIVRGNVIETNPVQGSQAPSGSQVEVIVSSGPAPIAVPNVGGLSQQDA
                     RDEIVQAGFP"
BASE COUNT          521 a          962 c          921 g          398 t
ORIGIN      
        1 ggttcgaccg gcgtcgagaa ctcctacagc ggcgatctct cgggccgcac gttcacgctg
       61 aacatcaaca atctcgagga cgtgttcgcg aaccgtcaga tcgtcggcac cgtcgtgctc
      121 acgttgtcaa agcaaatcca gcaagcggcg gcgggcgcac tcggtgacca tcccggcagc
      181 gtcgtcgcgc tcgacatcaa gaccggcgca gtgctcgcga tgtactccaa tcccacgttc
      241 gacccgaacc cgctggtcag tcacgacacc gcggaagcgc agcgggcgtt cgcgctcgat
      301 cagctcaatc cgggcaagcc gttgctccct cgcgcgtggc gcgagatctt tccgccgggc
      361 tcgacgttca agaccgtgac cgcagccatc gcgcaggaca cgaacaccga cgtcgacaag
      421 gtgttcccgg acgtgcgctt catcccgctg ccgcagacca cggcgatcct gcgcaacttc
      481 ggcgacgagc aatgcggcgg aacgatggaa gaaagcttca tcgtgtcgtg caacacgacg
      541 ttcgggcaga tcggcctcga ccttggtgag aagttcgcaa cggggatcaa gaacttcggc
      601 gtgcagaccg acccgccccg ccagtccggc tccggcatcg acccgccgat cgtgaagagc
      661 ctggggccgc cggaaggttc gttccagcag aaccagcccg cattcgcaca agcggccatc
      721 gggcaaaacg cgactgcggt cacgcccttg cagatggcga tggtcgccga gtcggtcgcg
      781 accggcggca cgatcctgca gccgcacgtc gtcgactgtg tgctcgaccc gaatggcgac
      841 cccgtgaaga aggtgccggt gcaggagtac aagcaggcga tgaccgccgc gaccgcggag
      901 acgatgcgca cgttcatgct cggcgtcgtc aacgacccga acggcacggg cagcgcggcc
      961 cggatcccgg gtgtcctcgt cgcgggcaag acgggaaccg ctcagacggg cgaggggcag
     1021 cgcaaccacg catggttcat cacgttcgcg cccgccgatc aaccgcggta tgcggtcgcg
     1081 gtgctcgtcg aacacggcgg cgccaacacc gaggcaaccg gcgggcgggt ggcggcgccg
     1141 attgcccgcc aggtgctcga agcattgctt gccagccagc ttcccccgtc gcgttgcggc
     1201 agtggccaac cataaaattc aacgagctgc ggatagggtt ttcgaccaat gacactggcc
     1261 ggccgggtgt tttccaaccg ctacgagatc cagcgcgaga tcgcgcaggg tgggatggcc
     1321 gaggtctacc tcgcgcgcga ccagctgctg aaccggccgg tcgccttgaa ggcgttgttc
     1381 ccggagtacg cccgcgagcc gtcgttcgtc gagcgcttcc ggcgggaggc gcaagccgca
     1441 gccaacctga acgaccccaa catcgtcgcc atctacgact ggggtcagga gagcggcacg
     1501 tacttcatcg tgatggagta cgtggaaggc cggtcgttgc gcgacctcat ccgcgccgaa
     1561 gccccagttg atcccaacca ggccgcggaa atcacggccg agatcgcgtc ggcgctcgcc
     1621 tacgcgcacc gcaacggggt cgtgcaccgc gacgtgaagc ccggcaacgt gttgctgacg
     1681 cgcagcggca acgtgaaagt caccgacttc ggcattgcgc gcgcgggggc gagcgacggc
     1741 ctgacccaga ccggctcggt catgggcacc gcgacctact tctcgcccga gcaggctcag
     1801 ggcctcccgg tcgacggccg cagcgacgtg tacgcgctcg gcgtcgtgct ctacgagatg
     1861 gtcactggcg tcgcgccgtt cacgggcgac tcccccgtgt cggtggcgta caagcacgtg
     1921 cgggaagacg tggtgccgcc ctcgcagcgc aacgcggaga ttccccccga actcgagcag
     1981 gtcatcatga ccgcgctcgc caaggacccg aaccagcgct accagactgc cgacgacttg
     2041 cgcgccgacc tcttgcgttt ccggcgcggg cgtccggtca cgggcgcggt gacggcaatc
     2101 gtgaccgagg ttccgacgac ggcgactgcc gccgcggcgg gggcatacgc ggccacgatg
     2161 gcgaccccgg tcgtcgacga ccgcgggcgc gcaacgaccg gtgcgcaata cccggccaag
     2221 aagaagaaca ccgcactcat ctgggtgctc acgctcctcg cgctcgcact ggtgatcggc
     2281 ggcatcctgt tcgcggcgac gcaactcggc gactcgcagg aacaggtgag cgtgcccaac
     2341 gtcgagggca agccgatcga cgaggcgcag tccatcctcg acgacgcgca cctcaagtac
     2401 gacatcacga acgtcaacac cccgctcgtc aaggccggca cggtggtcga gcagaccccg
     2461 aaggagggcg catccctcgc gcgcggatcg tcggtgaagc tgaccgtcag cgccggcgca
     2521 gaggagaagg cggtcccgac cgatctcgtg aaccatccgt tcgccgaagt cagcaagaag
     2581 ctcaccgacg cgggcttcac ggtgaaacgc accgaccgtc cgcacgacac gatcgtccgg
     2641 ggcaatgtga tcgagacgaa tccggtgcaa ggcagccagg caccatcggg gtcgcaggtc
     2701 gaggtgatcg tgtcgagcgg ccccgctccg atcgccgtcc cgaacgtcgg tggcctgtcg
     2761 caacaggacg cgcgcgacga gatcgtgcag gccggattcc cg
//
LOCUS       DASUGA010000865         5518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_664104, whole genome shotgun sequence.
ACCESSION   DASUGA010000865 DASUGA010000000
VERSION     DASUGA010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664104"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VFX21_17070"
     CDS             <1..544
                     /locus_tag="VFX21_17070"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5097736.1"
                     /translation="VAPSHRSMMEILFVALITRRRLRYMGKESVFKLPVLGTIFRWLG
                     GFPVARDGTDRKAVRDSMAMLDAGELLTVFPEGTRQNGPKIAPLQPGVAYLALRSNVP
                     IIPVGVAGSEEIFRSHPGRKKLNLPGFGRVVIVVGDPIMPPARTTSTVPRADVDALTT
                     TVAEGLQQAFDEAYRERDGE"
     gene            complement(580..1656)
                     /gene="ispH"
                     /locus_tag="VFX21_17075"
     CDS             complement(580..1656)
                     /gene="ispH"
                     /locus_tag="VFX21_17075"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX5097737.1"
                     /translation="MAVETVLLAEPRGFCAGVEMAIKALAWMVRAFDPPVYCYHEIVH
                     NRLVVDRFRAQGVIFVDDVADVPPGAPLMLSAHGSAPDVVAAAKTADRYVVNAVCPLV
                     TKVHHEAKVRARKGFTVLYVGHAGHDEAVGTLAVAPNAIRLVDGDTGLDAVIDDLDGD
                     QPVAMLAQTTLAHSEWTGLLDRTRSRFPELWTASRNDLCFATTNRQSALSAIAQHADA
                     VVVIGSANSSNTLALAKVAREGGCPIVLRVDGPDELHVDELGDARTVGVTAGASAPED
                     LVEAVIAKLAPTNGVEFVRVTDEDEYFPPPRELRELMPALDTVAALAFGGDPVAARAR
                     GGPFADDRALDASHVLSSLAREAR"
     gene            1698..3083
                     /locus_tag="VFX21_17080"
     CDS             1698..3083
                     /locus_tag="VFX21_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HEX5097738.1"
                     /translation="MPPARVVAVAPGSPAAAAGLLTGDEIVAVNGDELRDVIAYQLHV
                     DGAHVELDVRRGGLEQRVCVDKADGAPLGLELASAVFDRVRTCDNHCPFCFIYQLPKG
                     MRRSLYLKDDDYRLSFLYGNFTTLTRFTEADFERVVSERLGPLYVSIHATDPDVRTRL
                     LRNRRGATSLRWLGLLLDAGIEVHGQVVVCPGINDGAVLDETLLGVLDRFPRLATIGV
                     VPLGVSAHNTEPDMRPHTRAEAEAVLDTIGRWQDIYMRAFRKKGALGRRLVYASDEYY
                     LLAGRAFPALDEYDDCAQHENGIGMAQTFAAEVRAALAGDDRAGVGVRSGFFASVDGA
                     PADGYRADRTANVTVTLRRRGAPVAIVTGEFGARVLEPLLSELPGAVRLLPVANDFFG
                     GNIGVTGLMTGADLSRVLADAPAADRYLVPDVALSRGRFLDGMRPEELPRAVEIVPTD
                     GVSLVAALRAN"
     gene            3083..4420
                     /gene="der"
                     /locus_tag="VFX21_17085"
     CDS             3083..4420
                     /gene="der"
                     /locus_tag="VFX21_17085"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020462532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX5097739.1"
                     /translation="MGTRPMVAIVGRPNVGKSTLVNRIVGRRAAIVEEQPGVTRDRKE
                     FDATWRGREFGLVDTGGWLAPGDAGLTGEEPELAAKVSAQAEAAIEDADVVLFIVDVT
                     IGVTDEDARVARVLQRAPTPVLVVANKVDDERREADQWDFARLGLGDPIPVSALHGRL
                     AGELLDALVDALPPEADAEVDDGDDGIFSVAIVGRPNVGKSTLFNRIVGEERSVVHDM
                     PGTTRDAIDTIVETDDGPLRFVDTAGLRRKSRIEEGTEYYSLVRALEAIDRADAALFL
                     IDATEGVTHQDQRLAERVDAAGTALVLVLNKWDLLDTEQRADVRREVEDKLSFLTYAP
                     MLPISALTGRRVHQLLPALREAEAAYHQRIPTAALNRALRDAQLAHPPPVVKRHRPRI
                     LYATQGATDPPTFTLFATRSLSPQYLRYLERRLRESFDLGPTPIKLRVRRRAE"
     gene            4563..5144
                     /locus_tag="VFX21_17090"
     CDS             4563..5144
                     /locus_tag="VFX21_17090"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX5097740.1"
                     /translation="MRKGWVQRWRAARADRERGAVLVEAAIMIPLVLGLTFGMIEYGF
                     AFNEQGTVRAGTRTAARASSAVPSATTADFYEAALSTLDTSVHNLANGRADEVWIYQA
                     EEVGGNFQKPATCGVANCVTFKYVGDPDTGSFSRTPLGDTWDPGERHACDPADTDRVG
                     VYLVVEHTFLTEVRTLNLTSNTIMALEPSPAGC"
     gene            5156..>5518
                     /locus_tag="VFX21_17095"
     CDS             5156..>5518
                     /locus_tag="VFX21_17095"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX5097741.1"
                     /translation="MSRREREGGFVLLWFALSIVLLLGLAAFVVDLMHGYSQAQKVQN
                     SADAGALAGVVYMPGHDADAAALAESVVRNNVEDPAADVDVWTDTQCDPDCLPNQIQV
                     EVRASYPTYFAKIFGSRFD"
BASE COUNT          823 a         1935 c         1918 g          842 t
ORIGIN      
        1 cgtcgcgccc tcacaccggt cgatgatgga gatcctcttc gtcgcgctca tcacccggcg
       61 gcgcttgcgt tacatgggca aggagtcggt gttcaagctg cccgtgctcg gcacgatctt
      121 ccgctggttg ggtggttttc cggttgcgcg cgacggcacc gaccgcaagg cggtgcgcga
      181 ctccatggcg atgctcgacg cgggcgaact gctcaccgtg ttccccgagg gcacgcgcca
      241 gaacggtccg aagatcgctc cactccaacc aggtgtcgcg tacctcgcgc tgcgatcgaa
      301 cgttcccatc atcccggtcg gcgtggcggg cagcgaggag atcttccgca gccacccggg
      361 ccggaagaag ttgaacttgc cgggtttcgg ccgcgtcgtg atcgtggtcg gcgatccgat
      421 catgccgccc gcccgcacga cctcgacggt gccccgggcc gacgtcgacg cgctgaccac
      481 gaccgtggcc gaaggcctcc aacaggcctt cgacgaggcc tatcgcgagc gggacggaga
      541 gtgacgagtg aaaacggcga gtgatacgcg cggtcgtcgc tagcgcgctt cgcgcgcgag
      601 cgacgagagc acgtgtgacg cgtcgagcgc gcggtcgtcc gcgaacgggc cgccgcgcgc
      661 acgggccgcg accgggtctc caccgaacgc gagcgcggcg acggtgtcga gggccggcat
      721 caactcccgc agttcacgcg ggggcgggaa gtactcgtcc tcgtcggtga cgcgcacgaa
      781 ttcgacgccg ttcgtcggcg cgagcttggc gatgaccgct tcgacgaggt cttcgggtgc
      841 gctggcgccc gcggtcacgc cgacagtgcg ggcgtcacca agttcgtcga cgtggagctc
      901 gtcggggccg tcgacgcgca gcacgatcgg gcacccgccc tcccgggcga ccttcgcgag
      961 cgcgagcgtg ttcgacgagt tcgcgctccc gatcacgacc acggcgtcgg cgtgttgggc
     1021 gattgcgctc agcgcgctct gccggttggt cgtcgcgaag cacaggtcgt tgcgggaggc
     1081 ggtccacagc tcggggaagc ggctgcgggt gcggtcgagc aggccggtcc actcgctgtg
     1141 cgcgagcgtc gtctgggcca gcatcgccac cggctggtcg ccgtcgaggt cgtcgatcac
     1201 cgcgtcgagc ccggtgtcgc cgtcgacgag gcggatcgcg ttgggcgcaa cggccagggt
     1261 gccgaccgct tcgtcgtggc cggcatggcc gacgtacaac acggtgaagc ccttgcgggc
     1321 gcgcaccttc gcctcgtggt gcaccttcgt gacgagcggg cacaccgcgt tcacgacgta
     1381 gcggtccgcg gtcttggccg ccgcgacgac gtcgggggcg ctgccgtgcg cggagagcat
     1441 gagcggcgcg ccgggcggta cgtcggcgac gtcgtcgacg aagatcacgc cctgcgcccg
     1501 gaaccggtcg acgacgagcc ggttgtgcac gatctcgtgg tagcagtaca cgggcggatc
     1561 gaacgcccgc accatccatg cgagcgcctt gatcgccatc tcgacaccgg cgcaaaagcc
     1621 ccggggctcg gcgagcagca ccgtctccac tgccatgggg gccgagaata ccggtgctcc
     1681 tcccgtatcc tggccccatg ccgcccgcac gagtcgtcgc tgtcgcgccc ggatccccgg
     1741 cggccgcagc ggggttgctg accggcgacg agatcgtcgc ggtcaacggt gacgaactgc
     1801 gcgatgtcat cgcgtaccag ctgcacgtcg acggcgcgca cgtcgagctc gacgtccggc
     1861 gcggcggtct cgaacagcgc gtctgcgtcg acaaggccga cggtgcgccg ctcgggctcg
     1921 agctcgcaag cgcggtgttc gaccgcgtgc gcacctgcga caaccactgt ccgttctgct
     1981 tcatctacca gctgccgaag ggcatgcgtc gcagcttgta cttgaaggac gacgactacc
     2041 ggctgtcgtt cctgtacggg aacttcacga cgctgacgcg tttcaccgaa gctgacttcg
     2101 agcgcgtcgt gagcgaacgg ctcggtccgc tgtacgtcag catccacgcc accgatcccg
     2161 acgtacgcac gcggctgctg cgcaaccggc gcggtgccac gagcctgcgc tggttgggat
     2221 tgctgctgga tgccggcatc gaggtccacg gccaggtcgt ggtgtgcccc ggcattaacg
     2281 acggcgcggt gctcgacgaa acgctgctcg gcgtgctcga ccggttcccg cgtttggcga
     2341 cgatcggcgt cgtgcctctc ggcgtcagtg cgcacaacac cgagcccgac atgcggccac
     2401 acacgcgcgc cgaggccgag gcggtgctcg acacgatcgg ccgttggcaa gacatctata
     2461 tgcgggcttt caggaagaag ggtgcgctcg ggcgccgcct cgtttacgcc tccgacgagt
     2521 actacctgct cgcgggacgg gcgtttcccg ccctcgacga gtacgacgac tgcgcgcagc
     2581 acgagaacgg catcggcatg gcgcagacgt tcgcggccga ggtgcgcgcc gcgctggcgg
     2641 gcgacgaccg cgccggtgtg ggggtgcgca gcggcttctt cgcgagcgtc gacggcgcgc
     2701 ccgccgacgg gtaccgcgcc gaccgcaccg caaacgtcac tgtgacgtta cggcggcggg
     2761 gcgcgccggt cgcgatcgtc accggcgagt tcggcgcacg cgtgctggag cccttgctca
     2821 gtgagctccc gggcgccgtg cggctcttgc cggtggccaa cgacttcttc ggtggcaaca
     2881 tcggcgtgac cgggctgatg accggcgccg acctgtcacg cgtcctcgcc gacgcgcccg
     2941 ccgccgaccg ctatctcgtg cccgatgtcg cgctgtcgcg cggccgcttc ctcgacggca
     3001 tgaggcccga agaattgccg cgcgccgtcg agatcgtgcc caccgacggc gtctcgctcg
     3061 tcgcggccct gcgagcaaac tgatgggcac gagaccgatg gtcgcgatcg tcggtcgccc
     3121 caacgtcggc aagtcgacgc tcgtcaaccg catcgtcggc cgccgggccg cgatcgtcga
     3181 ggagcagcca ggggtcacgc gcgaccgcaa ggagttcgac gcgacctggc ggggacgtga
     3241 gttcggcttg gtcgacaccg gcggatggct cgcgccgggc gatgccggtc tgacgggcga
     3301 ggagccggag ctcgcggcga aggtcagtgc gcaggccgag gccgccatcg aagacgccga
     3361 cgtcgtgctc ttcatcgtcg acgtgaccat cggcgtcacc gacgaagacg cccgcgtcgc
     3421 gcgcgttctc cagcgggcgc cgacgccggt cctcgtcgtc gccaacaagg tcgatgacga
     3481 acggcgcgag gcggatcagt gggacttcgc gcggctcggg ttgggtgacc cgatcccggt
     3541 gtcggcgctg cacggccgcc tcgcgggtga gctgctcgat gcgctcgtcg acgcgctgcc
     3601 accggaagcc gatgccgagg tcgacgacgg tgacgacggc atcttctcgg tcgcgatcgt
     3661 cggtcgcccc aacgtcggca agtcgacgct gttcaaccgc atcgtggggg aggagcgctc
     3721 tgtcgtgcac gacatgccgg gcacgacgcg cgatgcgatc gacaccattg tcgagaccga
     3781 cgacgggccc ctgcgcttcg tcgacaccgc gggcctgcgc cgcaagagcc gcatcgagga
     3841 gggcaccgag tactacagcc tggtgcgcgc actggaggcg atcgaccggg ccgacgcggc
     3901 gctgttcctc atcgacgcga ccgagggtgt gacccaccaa gaccagcggc tggccgagcg
     3961 cgtcgacgcg gccggcaccg cgctggtgct ggtgctgaac aagtgggacc tcctcgacac
     4021 ggagcagcga gccgacgtgc ggcgcgaggt cgaggacaag ctctccttcc tcacctacgc
     4081 gcccatgctc ccgatctcgg cgctcaccgg gcggcgggtg caccagctgt tgcccgcgct
     4141 gcgcgaggcc gaggcggcct accaccaacg catcccgacc gcggcgttga accgcgcgct
     4201 gcgtgacgcc cagctggccc atccgccgcc cgtcgtcaag cgccaccggc cccgcatcct
     4261 gtacgccacg cagggcgcca ccgacccgcc gacgttcacg ctgttcgcca cccgcagcct
     4321 ctcgccccag tacctgcgct acctggagcg gcgcctgcgg gagagcttcg acctgggccc
     4381 cacaccgatc aaactgcgcg tccggcgtcg agcggagtga tccaccccaa tcggggggaa
     4441 tgagccaacc ggcctaatgc cactctcggt gcccttctgc caatatgggg tatctctccg
     4501 ggtcccgtga gggccagcca cggagaatcc gcggggtttc ggggcgtcga agggttgggg
     4561 tcatgcgcaa agggtgggtt cagcgttggc gcgccgcgcg cgccgaccgc gagcgcggcg
     4621 ccgtgctcgt cgaagcggcg atcatgattc ccctcgtcct cgggctgacg ttcggcatga
     4681 tcgagtacgg gttcgcgttc aacgagcagg gcaccgtccg cgccgggaca cgcaccgccg
     4741 cgcgcgcgtc gtccgcggtg ccgtcggcaa ccaccgctga cttctacgag gcggcgttga
     4801 gcacgctcga cacgtccgtc cacaatctcg ccaacgggcg cgcggacgag gtctggatct
     4861 atcaggccga agaagtcggc gggaacttcc agaagccggc cacctgcggg gtcgccaact
     4921 gcgtcacgtt caagtatgtc ggcgaccccg acaccggctc gttcagccgg acgccgctcg
     4981 gcgacacgtg ggatccgggc gagcggcacg catgcgaccc cgccgacacc gatcgcgtcg
     5041 gcgtgtactt ggtcgtcgag cacacgttcc tcaccgaggt gcggacgctc aacctgacca
     5101 gcaatacgat catggcgctc gagccgtcgc ccgccggatg ttgagttccc gtcgcgtgtc
     5161 gcgacgcgaa cgcgagggcg gcttcgtgct cctctggttc gcgctctcga tcgttctgct
     5221 gctcgggctc gcggcgttcg tcgtcgacct catgcacggg tactcgcagg cccagaaggt
     5281 gcagaactcg gccgacgccg gcgcgctggc gggtgtcgtc tacatgcccg ggcacgacgc
     5341 ggacgccgcc gcactcgccg aatcggtcgt caggaacaac gtcgaagatc ccgccgccga
     5401 cgtcgacgtc tggaccgaca cacagtgcga ccccgactgt ctgcccaacc agatccaggt
     5461 cgaggtccgg gccagttacc cgacgtactt cgcgaagatc ttcggcagcc gcttcgac
//
LOCUS       DASUGA010000866         2629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_108064, whole genome shotgun sequence.
ACCESSION   DASUGA010000866 DASUGA010000000
VERSION     DASUGA010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108064"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1731
                     /locus_tag="VFX21_17100"
     CDS             <1..1731
                     /locus_tag="VFX21_17100"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX5097742.1"
                     /translation="QLFRVAVAAPGSLTGRYPMGNTGGADVSAFAPMPIPDDLRTLLE
                     PDATFSRDTTGLDDARLPETVELADGDIYDLRIGAVAKDLDGDRVRMLAYNGSIPGPT
                     LRVMQGSEVVVRVRNDGDTEATVHWHGLRLANEYDGVPYETQDPIAIGGEFTYRLQFP
                     DAGVYWYHPHIREDYGLDMGLYGNIVVAPADPDYWLPAHREVVVTLDDVLVQDGAIVA
                     YKHAGPTHVAMGRFGNVMLTGGATDLALSAARGEVVRFYFTNTANTRLFNVALPGARM
                     KLVGSDSGRYEHETFVEEVLLAPSERAVADVLFDTAGECLVEHRTPDRTYVLGRVAVA
                     DVDASPSYADDWSRLRTSDELTAERARVAADLQRDPDKLLAFTSSMPLLYGDTDTAAS
                     AWTCPMHPEVVSATPDTCPKCGMKLVPDTGDRPAHEHEHAHVHAHDTGDGLEWEDLMP
                     EINAASDRENMIWKLVDLDTGRENWEIDWSFAVGDRVKIRLVNGMDQDHPMHHPFHVH
                     GAGRFLVLARDGVAEPNLAWKDTVLVRAGETVDILLDVTNPGLWMAHCHIAEHNQSGM
                     MFSFPVRERA"
     gene            complement(1744..>2629)
                     /locus_tag="VFX21_17105"
     CDS             complement(1744..>2629)
                     /locus_tag="VFX21_17105"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5097743.1"
                     /translation="LALAHVEAEKACAELSAINVPNAAAAYAEVGDIRRRLGDLAGSE
                     EAFARAEELSGRSCSGFALLRLAQGRVDAAIRAIDDCLHDTSWNRLARARVLPAHVHI
                     AIAAGALDAARASADELDGIVADYDTPVLRATALTTRGRVLLAEREYRAAATHLRDAL
                     AQWKVLEVPYETASTGTLLGQALRESADEEGATSAFKAADAIFEQMGARLDAAHTRDL
                     QVQAPTTPAPCGLSAREVEVLRLIASGHANKTIASELFLSEKTVSRHVSNIFAKIGVS
                     SRASATAFAFEQGLAGGS"
BASE COUNT          415 a          922 c          896 g          396 t
ORIGIN      
        1 cagctctttc gcgtcgccgt cgccgcgccc gggtcgttga ccgggcgcta cccgatgggc
       61 aacaccggcg gcgccgacgt gagtgcgttc gcacccatgc ccatccccga cgacctgcgc
      121 acactgctcg aacccgatgc gacgttctcg agggatacga cgggactcga cgacgcgcga
      181 ctgccggaga ccgtcgagct cgccgacggc gacatctacg acctgcgcat cggcgccgtc
      241 gccaaggatc tcgacggcga tcgggtccgc atgctcgcat acaacggttc gattccgggg
      301 ccgacactgc gtgtgatgca gggctcagag gtcgtcgtgc gcgtgcgcaa cgacggcgac
      361 accgaagcga ccgtgcactg gcacggtctg cgtctcgcga acgaatacga cggcgtgccc
      421 tacgagacgc aggacccgat cgcgatcggc ggcgagttca cgtatcggct gcagttcccg
      481 gacgcgggcg tctactggta ccacccgcac atccgcgagg actacggcct cgacatgggc
      541 ctgtacggca acatcgtggt cgcgcccgcc gaccccgatt actggttgcc cgcgcaccgc
      601 gaggtcgtcg tcacgctcga cgacgtgctc gtgcaagacg gcgcgatcgt cgcctacaag
      661 cacgcgggcc cgacgcacgt cgccatgggg cgattcggca acgtcatgtt gaccgggggt
      721 gcaaccgacc tcgcactctc ggccgcgcgt ggtgaagtcg tgcggttcta tttcaccaac
      781 accgcgaaca cgcgactgtt caacgtcgca ttgccgggcg cgcgcatgaa gctcgtcggg
      841 agcgacagcg gacggtatga acacgagacc ttcgtcgagg aggtcttgct cgcgccttcg
      901 gagcgtgcgg tcgccgacgt actgttcgac acggccggcg agtgtctcgt cgagcaccgc
      961 acgcccgatc gcacctacgt gctcggtcgc gtggcggtcg cggacgtcga cgcgtcaccg
     1021 tcgtacgccg acgattggtc gcgactgcgc acgagcgacg agctcaccgc ggaacgggcc
     1081 cgcgtcgcgg ccgacctgca gcgcgatccc gacaagctgc tcgcgttcac gtcgtcgatg
     1141 ccgctgctct acggcgacac cgacactgcc gcgagcgcgt ggacctgccc gatgcatccc
     1201 gaggtcgtct ccgccacgcc cgacacgtgt ccgaagtgcg gcatgaagct cgtgccggac
     1261 acgggcgacc gaccggcgca cgagcacgag catgcacacg tccatgccca cgacaccggc
     1321 gacgggctcg aatgggaaga cctcatgccc gagatcaacg ccgcgtcgga ccgcgagaac
     1381 atgatctgga agctcgtcga tctcgacacc ggccgggaga actgggagat cgactggtcg
     1441 ttcgccgtcg gcgaccgcgt gaagatccgg ctggtgaacg gcatggacca ggaccacccg
     1501 atgcaccacc cgttccatgt gcacggcgcg ggccggttcc tcgtgctcgc gcgcgacggt
     1561 gtggccgagc cgaacctcgc gtggaaggac accgtgctcg tgcgggccgg cgagaccgtc
     1621 gacatcctcc tcgacgtgac gaaccccggg ctctggatgg cgcactgtca catcgccgag
     1681 cacaaccaga gcgggatgat gttcagcttc ccggtgcgcg agcgcgcgta ggcgtctccg
     1741 cgatcagctg ccgccggcga ggccttgttc gaacgcgaac gcggtcgcgg aagcgcgcga
     1801 ggagacgccg atcttcgcga agatgttgga gacgtgccgg gagacagtct tctcgctgag
     1861 gaagagctcg ctcgcgatcg tcttgttggc gtggccggaa gcgatgagtc ggagcacctc
     1921 gacttcacgg gcgctcagcc cgcacggagc gggggtcgtc ggcgcctgca cttgcaggtc
     1981 gcgtgtgtgc gcggcgtcga ggcgagcgcc catctgctcg aagatcgcgt ccgcggcctt
     2041 gaacgcgctg gtggcaccct cctcgtcggc gctctcccgc agcgcctggc cgagcagcgt
     2101 tcccgtcgac gcggtctcgt acggaacctc gagcaccttc cactgcgcca gcgcgtcacg
     2161 aaggtgcgtc gcggccgcgc ggtactcccg ctcggccagc aacactcgcc cgcgtgtggt
     2221 cagcgcagtc gcgcgcagga cgggcgtgtc gtagtcggcg acgatgccgt cgagctcgtc
     2281 ggccgacgcg cgtgcggcgt cgagcgcgcc ggcggcgatg gcaatgtgca cgtgggcggg
     2341 cagcactcga gcacgcgcga gccggttcca cgacgtgtcg tgcaagcagt cgtcgatggc
     2401 tcggatcgcg gcgtcgacgc gcccctgcgc gaggcgcagc agcgcgaacc cggaacagct
     2461 ccgcccactc agctcctcgg ctcgcgcgaa cgcctcttcg ctgccggcga gatcgccgag
     2521 ccgccggcga atgtcgccca cttccgcgta ggccgccgcc gcgttcggga cgttgatcgc
     2581 gctgagctcg gcgcacgcct tctcggcctc gacgtgtgcg agcgcgagc
//
LOCUS       DASUGA010000867         2341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1639253, whole genome shotgun sequence.
ACCESSION   DASUGA010000867 DASUGA010000000
VERSION     DASUGA010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639253"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFX21_17110"
     CDS             <1..201
                     /locus_tag="VFX21_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5097744.1"
                     /translation="PHAAIDECTTADIPRDAVVVTYCWGPHCNGATKAAARFAALGFR
                     VKEMLGGITGWQTEGYELDGPG"
     gene            complement(522..1133)
                     /locus_tag="VFX21_17115"
     CDS             complement(522..1133)
                     /locus_tag="VFX21_17115"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5097745.1"
                     /translation="MSESETFHEDNRYWQDQFDSRRIADRIDELLVSETIDQHAKAFI
                     ESRDMFFLATCDAEGHPQCSYKGGDPGFVRVVDERTIAFPVYDGNGMFLSLGNIRQQA
                     EVGMLFVDFENPNRLRLNGTASVDEQDPLLASFTGARLVVRVRTRQVFPNCGRYIHKL
                     QLVERSPFVPRPEAPPPIPDWKRMTWAQEFLPNGDPAKDATPI"
     gene            1214..1822
                     /locus_tag="VFX21_17120"
     CDS             1214..1822
                     /locus_tag="VFX21_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097746.1"
                     /translation="MLFQQRFWAGIRDGSITLTFRRWKRQLVLPDRRYRTAAGMLEGE
                     KVTVVDPARITKADARRAGFASADALRSELRGDESLPTYRIQFHLVTEPDPRSVLAAD
                     DGLTADDVAEIDSRLDRLDAASTHGPWTAAVLDVIRERPGTRAPDLAASFGRETQPFK
                     TDVRKLKNLGLTLSLTVGYRLSPRGEAYVSSTRRAKPPRQDA"
     gene            1819..>2341
                     /locus_tag="VFX21_17125"
     CDS             1819..>2341
                     /locus_tag="VFX21_17125"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEX5097747.1"
                     /translation="MTHVGILAPMKPELAPLLKRTQLTREQRGDFIVQRGRIGRADVT
                     ATITGIGMTPAQKTTERLLDDFGVDHVLVMGIAGGVDAKLVIGETFWPEVVVDGRDER
                     EYRQKPFDGSVPRGLIISSDALEMHDALDSLRERGVTAVDMETGAVAAVCEARGVAWS
                     AIRSISDKVGDKAI"
BASE COUNT          361 a          790 c          808 g          382 t
ORIGIN      
        1 ccgcacgcgg ccatcgacga atgcacgacc gcggacatcc cgcgcgacgc ggtcgtcgtg
       61 acgtactgct gggggccgca ctgcaacggc gcgaccaagg ccgcggcccg gttcgcggca
      121 ctcggcttcc gggtgaagga gatgctcggc ggcatcacgg gctggcagac ggagggttac
      181 gaactcgacg gaccgggttg acgtggggca ggcttcacgt accgcttgac aacgacgcaa
      241 atgcggcgag actggcggta cttgccgtgt cggcccgtgg cgccccgtgc cgatgcggtc
      301 gctcgggtcg ccggccgtac ggaccgcccg ccgtcgcccg gcgacccgag ctatccctct
      361 ttgtcgctct cgatgtttct ttgtcgcctt cgacctgctc gcgtgaggcg gcccggattg
      421 ttggccggcc tcaggactcc ggcgagcgcc tccgcaagct ccggcgccgc gccgtgtgac
      481 ttctcgcgac gctgcccgta cttcgtcgct gtcgatgctt cctagatcgg ggttgcgtct
      541 ttcgctgggt cgccgttggg caggaactcc tgcgcccatg tcatgcgctt ccagtccggg
      601 atcggcggcg gcgcttcggg ccgcggcacg aacggcgaac gctcgacgag ttgcaacttg
      661 tggatgtagc gcccgcagtt cggaaagacc tggcgggtgc ggacccgcac gacgagacgc
      721 gcacccgtga acgacgcgag caacggatct tgttcgtcga ccgacgcggt gccgttcagc
      781 cggagacgat tggggttctc gaagtcgacg aacaacatgc cgacctccgc ctgctgacgg
      841 atgttgccca gcgacaggaa catgccgttg ccgtcgtaga ccgggaacgc gatcgtgcgc
      901 tcgtcgacga cgcgcacgaa gcccgggtcg ccacctttgt acgagcactg cggatggccc
      961 tcggcgtcgc acgtcgcgag gaagaacatg tcgcgcgact cgatgaacgc tttcgcatgt
     1021 tggtcgatgg tctcggagac gagcagttcg tcgatgcgat ccgcgatgcg gcgggagtcg
     1081 aactggtcct gccagtaccg gttgtcctcg tggaacgtct cgctctcgct catgcccttg
     1141 agcgtaccca ccccccgacc ccacccggcc gccccgtaat gcacgacggg cttcgcccgc
     1201 cgtacgttta agagtgctct ttcagcagcg cttctgggcc ggcatccgcg acggctcgat
     1261 cacgttgacg ttccggcgat ggaagcgtca gctggtgctg cccgaccggc gctaccgcac
     1321 cgcggcgggg atgctcgagg gcgagaaggt cacggttgtc gatccggcgc gcatcacgaa
     1381 ggccgacgcg cgccgggccg ggttcgcttc tgccgacgca ttgcggtccg agctgcgcgg
     1441 tgacgagtcg ctgccgacgt accgcatcca gttccatctc gtcaccgagc ccgacccgcg
     1501 ctcggtgctc gccgcggacg acgggttgac cgcggacgac gtcgccgaaa tcgatagtcg
     1561 cctcgaccgg ctcgacgccg ccagcacgca cgggccgtgg accgcggccg tgctcgacgt
     1621 gatccgtgag cggccgggga cgcgcgcgcc cgatctcgcg gcatccttcg ggcgcgagac
     1681 gcagccgttc aagaccgacg tccgcaagct caagaacctc gggctcacgc tgagcctgac
     1741 ggtcggctac cggctgtcgc cgcgcggcga ggcgtacgtg tcctcgacgc ggcgcgcgaa
     1801 accgcctaga caggacgcat gacgcacgtc ggcattctcg cccccatgaa gcccgagctc
     1861 gctccgctgt tgaagcgcac gcaactcacg cgcgaacaac gcggcgactt catcgtgcag
     1921 cgcggccgga tcggcagggc cgacgtgacc gcgacgatca cgggcatcgg catgacgccc
     1981 gcgcagaaga cgaccgagcg cttgctcgac gacttcggcg tcgatcacgt tctcgtgatg
     2041 ggcatcgcgg gcggcgtcga cgcgaagttg gtgatcggcg agacgttctg gcccgaggtc
     2101 gtcgtcgacg ggcgcgacga gcgcgagtac cggcagaagc cgttcgacgg ctccgtaccg
     2161 cgggggctga tcatctcgtc cgatgcgctc gagatgcacg atgcgctcga cagcctgcgc
     2221 gagcgcggtg tcaccgcggt cgacatggag acgggtgcgg tcgcggcggt gtgcgaagca
     2281 cgtggtgttg cgtggtcggc gatccgcagc atcagtgaca aggtcggcga caaggcgatc
     2341 a
//
LOCUS       DASUGA010000868         2651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_665842, whole genome shotgun sequence.
ACCESSION   DASUGA010000868 DASUGA010000000
VERSION     DASUGA010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665842"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VFX21_17130"
     CDS             <1..390
                     /locus_tag="VFX21_17130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097748.1"
                     /translation="ARIAGTLEARSIAQLMVPLTVSVPEWTTVGDLPAALPPGPLPSA
                     VLLTGFGGSPTALLQTGALRNAHAHQRVRELAIAVSDITAVPPTMSAHDALANGVPVV
                     VIDDGHIVGVVGIDEVRRAAGRDVVNV"
     gene            complement(380..910)
                     /gene="thpR"
                     /locus_tag="VFX21_17135"
     CDS             complement(380..910)
                     /gene="thpR"
                     /locus_tag="VFX21_17135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX5097749.1"
                     /translation="MARAFVAVTLPEPVLDAVDAATAGLELPDARRTPREQWHLTLQF
                     LGNHVDLDATAAALETLDAPAGRLGLGGAGAFPKPARATVLWIGLREGVLYLSDLAER
                     VSRATGVPLEHRPHRPHLTIARPKRATDVRGVVAALDPTSLGDAWDVTDITLFESVLG
                     SSGARHTPVRTIALRR"
     gene            complement(929..1273)
                     /locus_tag="VFX21_17140"
     CDS             complement(929..1273)
                     /locus_tag="VFX21_17140"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5097750.1"
                     /translation="MEIQVNVDCADLDTMVRFYTAALGYEPYGRAGDHYASIVHPTLP
                     KLVFQKVPEPRIGKNRVHLDLIVGDAIEAEAERFVGLGAQRLSDGVVSEYGVSWIVMA
                     DPEGNEICLCDG"
     gene            complement(1273..2517)
                     /locus_tag="VFX21_17145"
     CDS             complement(1273..2517)
                     /locus_tag="VFX21_17145"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX5097751.1"
                     /translation="MRCEVLAIGTELLLGQIVDTNSAWIGEQLAASGIDSYEHRAIGD
                     NQDRIVAAMRDLLSRSDALLICGGLGPTQDDLTRDAIAEVMGVPLVRDMSLAEHIAMM
                     FRARLRDMPENNLRQADIPEGGNAIDNPLGTAPGLLCELDGGKIVYAVPGVPYEMQEM
                     VRTHVIPDLLRRSGEAAAIVSRSLKTWGTSESGLAEQIAHRLDALDGSNTTIAFLARG
                     IEGLVVRITAKAASEPEARALVEIEEKELRAILGDLVFGVDDETMEHAVVHRLKARGW
                     TLGAAESVTGGLIGARIVNVEGASEVFRGSIASYATDVKRSVLGVTAASVVSEDAARE
                     MAEGAQRVLGADVGIAITGVAGPTEQDGVAVGTVFFGLAIPGVPTETISFRLPGDRER
                     IRQFGTISVLNLLRLRLDALGA"
BASE COUNT          408 a          973 c          866 g          404 t
ORIGIN      
        1 gcgcgcatcg ccggcacgct cgaagcccgg tcgatcgcgc aactcatggt cccgctcacg
       61 gtgtcggtac ccgagtggac cacggtcggc gacctgcctg ccgcgctccc gccgggcccg
      121 ctcccctcgg ccgtattgct gaccggcttc ggcggatcac cgaccgcgct gttgcagacc
      181 ggcgcgctgc gcaacgccca cgcacaccag cgtgtccgcg agctcgcgat cgccgtctcc
      241 gacatcactg ccgtcccgcc cacgatgtcg gcccacgacg cgctcgccaa cggcgtgccc
      301 gttgtcgtga tcgacgacgg tcacatcgtc ggggtcgtcg gcatcgacga agtgcggcgg
      361 gccgccggcc gcgacgtcgt caacgtctga gtgcgatcgt acggaccggc gtgtggcgcg
      421 cgcccgatga tcccaggacg ctctcgaaca aggtgatgtc ggtgacgtcc caagcgtcac
      481 cgagagacgt cggatccaac gcggcaacga ccccgcgcac atccgtcgcg cgcttcgggc
      541 gtgcgatcgt gaggtgcggc cggtggggcc ggtgctcgag cggcacaccc gtcgcgcgcg
      601 acacccgctc ggcgagatca ctcaggtaca agacaccctc gcgcaggccg atccacagca
      661 ccgtcgcgcg cgcgggcttc gggaacgcgc ccgcgccacc gagcccgagg cggcccgcgg
      721 gcgcgtccag cgtttcgagc gcggccgcgg ttgcgtcgag atccacgtgg ttgccgagga
      781 actggagcgt gagatgccac tgctcgcgcg gggtccgtcg tgcgtccggc agctccagtc
      841 cggctgtcgc cgcgtcgacc gcgtccagta ccggttcggg cagcgtcacc gcgacgaacg
      901 cgcgcgccac gccgctagtg ccccgtcact aaccgtcgca caggcagatc tcgttgcctt
      961 ccggatcggc catgacgatc cacgagacgc cgtactccga cacgacgccg tccgacaggc
     1021 gctgggcacc cagcccgacg aaccgttcgg cctcggcttc gatcgcgtca ccgacgatca
     1081 ggtcgaggtg cactcggttc ttcccgatgc gcggttcggg caccttctgg aacacgagct
     1141 tcggcaacgt cggatgcacg atcgacgcgt agtgatcgcc ggctcgtccg tacggctcgt
     1201 agccgagcgc cgccgtgtag aagcggacca tcgtgtcgag gtccgcgcag tcgacgttga
     1261 cctggatctc catcacgcgc ccaacgcgtc gagccgcagt cgcagcaagt tcaacaccga
     1321 gatcgtcccg aactgacgga tgcgttcacg gtcaccgggc aggcggaacg agatcgtctc
     1381 ggtcggcacc cccgggatcg cgagcccgaa gaagacagtg ccgacggcga cgccgtcttg
     1441 ctcggtcggt cccgcgacgc cggtgatcgc gatgccgacg tcggccccga gcacgcgctg
     1501 tgcaccctcg gccatctcgc gcgcggcgtc ctcgctgacg acgctcgcag ccgtcacgcc
     1561 caagacggag cgcttcacgt cggtcgcata cgacgcgatc gagccgcgaa acacttcgct
     1621 tgcgccttcg acgttgacga tgcgcgcgcc gatcagcccg ccggtcacgg actccgccgc
     1681 gccgagtgtc cacccgcgcg ccttcaaccg gtgcacgact gcgtgctcca tcgtctcgtc
     1741 gtcgacaccg aacaccaggt cgccgaggat cgcacgcagc tccttctcct cgatctcgac
     1801 gagcgcccgc gcctccggct ccgacgcggc cttcgccgtg atgcggacga ccagcccttc
     1861 gatgccacga gcgaggaagg cgatcgtcgt gttcgacccg tcgagcgcgt cgagccggtg
     1921 cgcgatctgc tccgccagcc ccgactcgga cgtgccccac gtcttcaacg accgcgacac
     1981 gatcgcggcc gcctcacccg accggcgcaa caggtcgggg atgacgtgcg tgcgcaccat
     2041 ctcctgcatc tcgtacggca cgccgggcac tgcgtacacg atcttcccgc cgtcgagctc
     2101 acacaagagg ccgggcgcgg tgccgagcgg gttgtcgatc gcgttgccgc cctcggggat
     2161 gtcggcctgg cgcaagttgt tctcgggcat gtcgcgcaac cgggcgcgaa acatcatcgc
     2221 gatgtgctcg gccaggctca tgtcgcgcac gagcggaaca cccatcactt ccgcgatcgc
     2281 gtcgcgcgtg aggtcgtctt gggtcgggcc gaggcccccg cagatgagca gcgcatcgga
     2341 acgtgacaag aggtcgcgca tcgccgcgac gatgcggtcc tggttgtcgc cgatcgcgcg
     2401 atgctcgtag gagtcgatgc ccgacgccgc gagctgctcc ccgatccacg cactgttggt
     2461 gtcgacgatc tgcccgagca gcagttcggt cccgatggcc agcacttcgc atcgcatcgc
     2521 gcggatgcta tgtgtcttgg cgctgccagt cgacgtagcc gtgccgcagg atcgcaaggc
     2581 ccgacacgac agtgagcgcg accgcgatgt agagcacgac gtgctggaac acgtcggcgt
     2641 ccgcggtgag c
//
LOCUS       DASUGA010000869         2838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_388693, whole genome shotgun sequence.
ACCESSION   DASUGA010000869 DASUGA010000000
VERSION     DASUGA010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388693"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VFX21_17150"
     CDS             complement(<1..573)
                     /locus_tag="VFX21_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017013416.1"
                     /note="extradiol catechol dioxygenase that catalyzes the
                     oxidative cleavage of substituted catechols; part of the
                     bacterial aromatic compound degradation pathway; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HEX5097752.1"
                     /translation="MAELVGAIGTSHIPAVGVAIDKGRTEEPYWAPYFAKLPPVREWM
                     AKTKPDVCIVVYNDHANAFSLELIPTFALGTAARFGIADEGFGKRPVPDVIGYPELAW
                     HLGESLILDEFDMTLVQEMEVDHGLTVPLSVAFGQPDEWPCKVIPLAVNVVQYPPPTG
                     NRCYRLGQAIRRAIDSYPGNERVVIFGTGGL"
     gene            complement(566..967)
                     /gene="ligA"
                     /locus_tag="VFX21_17155"
     CDS             complement(566..967)
                     /gene="ligA"
                     /locus_tag="VFX21_17155"
                     /EC_number="1.13.11.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018579 - protocatechuate 4,5-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 4,5-dioxygenase subunit alpha"
                     /protein_id="HEX5097753.1"
                     /translation="MHEYDDIPGTFVFDGQRSREGYWLNMFCMSLREEANRDAFRADE
                     EAYLDKYALTPEQRQAVRQRDWLRMLELGGNIYYTFKLAACDGLTFQQLAAKQTGVSE
                     DEYVAMMVGGGRSIDGNRSRAGSTGGSDSHG"
     gene            1096..2229
                     /locus_tag="VFX21_17160"
     CDS             1096..2229
                     /locus_tag="VFX21_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEX5097754.1"
                     /translation="MSDEIRPFTIDVPDSVLDDLRVRLAHTRWPDRETVADWSQGIPL
                     EYVQEVARYWADGYDWRRRERALNRFDQYVTEIDGVDIHFIRQRSPHADALPLLITHG
                     WPGSIVEFHKVIEPLVDPVAFGGDASDAFDVVAPSLPGFAFSGKPPAAGWGVERIGAA
                     WTKLMARLGYDRYVAQGGDWGSAVTTQVGAGDAAHCAAIHITLNMSGRPVGQPTEPDE
                     LHALACIEQYLKWDSGYSTQQRTRPQTLGFGLADSPAGQLAWILEKFWAWTDCDGHPE
                     NALTRDEMLDNVMLYWVTNSATSSARLYWESFGRGSRPTVSVPTGFAVYPKEIVPPVR
                     SWCEERYPNIVHWRAYDKGGHFAAFEVPETFVDDVREWARLVR"
     gene            complement(2234..2527)
                     /locus_tag="VFX21_17165"
     CDS             complement(2234..2527)
                     /locus_tag="VFX21_17165"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEX5097755.1"
                     /translation="MIRHVVLFQFADDTTVEQIDDYEQSLVDYVATLQGVEDYVIGRD
                     ARINPGTYDFSIVAEFSDEAAFREYFDGERHKEIQRDSASMIVAKASSQSRFE"
     gene            2567..>2838
                     /locus_tag="VFX21_17170"
     CDS             2567..>2838
                     /locus_tag="VFX21_17170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX5097756.1"
                     /translation="MSLISVEDLAARLADPGSANSTVVCDCRFYLADHHQGRHEYDEA
                     HIPAARFVDVHLDLAGGPGGGRHPLPTVDAFTQLLGRLGIAPSTFVV"
BASE COUNT          446 a          974 c          933 g          485 t
ORIGIN      
        1 cagaccgccc gtgccgaaga tcacgacccg ctcgttaccg gggtacgagt cgatcgcgcg
       61 ccggatcgcc tggcccagcc ggtagcaccg attgcccgtc ggcggcgggt actgcacgac
      121 gttgacggcg agcgggatca ccttgcacgg ccattcgtcg ggctgtccga acgccaccga
      181 cagcggcacg gtgaggccgt ggtcgacctc catctcctgc acgagcgtca tgtcgaactc
      241 gtcgaggatc agcgactcgc cgagatgcca cgccagctcg ggatacccga tcacgtccgg
      301 cacggggcgc ttgccgaagc cctcgtcggc gatgccgaag cgcgccgcgg tgccgagcgc
      361 gaacgtcggg atcagctcga gcgagaaggc gttggcgtgg tcgttgtaga cgacgatgca
      421 cacatcgggc ttcgtcttcg ccatccactc ccgcaccggc ggcagcttgg cgaagtacgg
      481 cgcccagtac ggctcttccg tgcggccctt gtcgatcgcc accccgacgg cggggatgtg
      541 cgacgtgccg atcgcgccca cgagctcagc catggctgtc actccctccc gtgctacccg
      601 cccgcgagcg gttgccgtcg atgctgcggc caccgccgac catcatcgcc acgtactcgt
      661 cttcgctgac gccggtctgc ttggcggcca gctgctggaa cgtcaggcca tcgcaggcag
      721 cgagcttgaa cgtgtaatag atgttcccgc cgagctcgag catgcgcagc cagtcgcgct
      781 ggcgcaccgc ctggcgttgc tcgggcgtga gcgcgtactt gtcgaggtac gcctcctcgt
      841 cggcgcggaa cgcgtcgcgg ttggcctcct cacgcagcga catgcagaac atgttgagcc
      901 agtacccctc gcgactgcgc tgaccgtcga acacgaacgt gcccggaatg tcgtcgtact
      961 cgtgcacacc acgatcgtac ggcggttgcc ggccgcgatg ccctccatcg ttcgtgcctc
     1021 ggaaaggagt gagcgatcat gggtctatag cccatgatcg ctcacgcaat tcgggggcgc
     1081 gtaccgtgag cgttcatgtc cgacgagatc cgtcctttca ccatcgacgt gccggactcg
     1141 gtgctcgacg acctgcgtgt ccgcctcgcg cacacgcgtt ggccggaccg tgagacggtc
     1201 gcggactggt ctcagggcat tccgctcgag tacgtccagg aggtcgcgcg ctactgggcg
     1261 gacggctacg actggcgacg gcgcgaaagg gcactcaacc gcttcgacca gtacgtcacg
     1321 gagatcgacg gcgtcgacat ccacttcatc cgtcaacgct caccgcacgc ggacgccctt
     1381 ccactgttga tcacgcatgg ctggccgggc tcgatcgtcg agttccacaa ggtgatcgag
     1441 ccgctcgtcg acccggtcgc gttcggcggg gatgcatccg atgccttcga cgtggtcgcg
     1501 ccatcgcttc ccgggttcgc attctccggc aagccgccag ccgccggttg gggcgtcgaa
     1561 cgcatcggcg ccgcatggac aaagctcatg gcgcgcctcg gatacgaccg ctacgtcgcg
     1621 cagggcggcg actggggctc ggcagtgacg acccaagtcg gtgccggcga tgccgcgcat
     1681 tgcgccgcga tccacatcac gctcaacatg tccggccgcc cggtggggca accgaccgag
     1741 cccgacgagc tgcacgcgct ggcgtgcatc gagcagtacc tcaagtggga ctcgggctac
     1801 tcgacgcaac agcggacgcg cccgcagacg ctcggattcg gcctcgccga ctcgccggcc
     1861 gggcagctgg cttggatcct cgagaagttc tgggcgtgga ccgactgcga cggccatccc
     1921 gagaacgcgt tgacgcgcga cgagatgctc gacaacgtga tgctgtactg ggtcaccaac
     1981 tcggcgacat cgtcggcgcg cctgtattgg gagagcttcg gccgcgggtc acgaccgacg
     2041 gtctcggtgc cgaccggttt cgcggtctac ccgaaggaga tcgtcccgcc ggtgcggtcg
     2101 tggtgcgagg agcgctaccc gaacatcgtg cactggcgcg cctacgacaa gggcggccac
     2161 ttcgccgcgt tcgaggtgcc cgagacgttc gtcgacgacg tgcgcgagtg ggcccggctc
     2221 gtgcgctgag gggctattcg aaccgggatt gcgacgacgc cttggcgacg atcatcgacg
     2281 ccgagtcgcg ctggatctcc ttgtgccgct cgccgtcgaa gtactcgcgg aacgcggcct
     2341 cgtcgctgaa ctcggcgacg atcgagaagt cgtacgtgcc cgggttgatg cgcgcatcac
     2401 gcccgatcac atagtcctcg acaccttgga gcgtggcgac gtagtcgacg aggctctgct
     2461 cgtagtcgtc gatctgctcg acggtcgtgt cgtcggcgaa ctggaagagc accacatggc
     2521 ggatcaccgc agcagtctcg ccgaagtcgt tggctagcgt cgcaccgtgt cattgatctc
     2581 tgtcgaggac ctcgccgcgc ggctggccga ccccgggtcg gcgaattcga ccgttgtgtg
     2641 cgattgccgc ttctaccttg ccgatcacca ccagggtcgc cacgagtacg acgaggcgca
     2701 cataccggcg gcgcggttcg tcgacgtcca cctcgacctc gccggcggcc cggggggagg
     2761 tcgccatccg ctcccgacgg tcgacgcatt cacgcaactg ttgggccggc tcggcatcgc
     2821 cccctcgacg ttcgtcgt
//
LOCUS       DASUGA010000870         3325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_353341, whole genome shotgun sequence.
ACCESSION   DASUGA010000870 DASUGA010000000
VERSION     DASUGA010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353341"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1153
                     /locus_tag="VFX21_17175"
     CDS             146..1153
                     /locus_tag="VFX21_17175"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX5097757.1"
                     /translation="MKVAVIGAGSWGTTVAIIAAANSPTVLWARRPDLAERMTATREN
                     PDYLPGIEFPEALAVTGSLEAACDRADVIVLAVPSHGFRDVLNGAQSFIGVDVPVISL
                     SKGIEQGTLQRMTEVVGEVLEGHHPARIGVLTGPNLAKEVAGGQPTASVVAIGDEVVA
                     ERLQRAFATPTFRVYTNPDVVGCEMAGALKNVLAIGAGIADGLGYGDNTKAALMTRGL
                     AELGRLGVAMGGDPLTFAGLAGMGDLIATCSSPQSRNRHVGFELGRGHSLDAIVGEMN
                     MVAEGVKSTAAVIELAQRHDVEMPLSSFVGRVLYENARPADLVPELMLRKAKPELHGM
                     R"
     gene            1153..2346
                     /locus_tag="VFX21_17180"
     CDS             1153..2346
                     /locus_tag="VFX21_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097758.1"
                     /translation="MTRLGTIGGAATATVDAGGCVTAGARTLDWWIGADDRWHEPAGE
                     AGRRQSRLGPGPVVETSIRVPGGDAHQRVYAVTAPDAIVMEVENASPAPFVFTAVLRA
                     SGTVALADRVLTVDGVPVLTVPRPPLRWSVGRLTGSNSDPVRRPTNLVRDEVFGGEAQ
                     TGPFASVTGDVEVALCFSVSHRTRSRVALASEPVDLVALASPDETARGWEHQLDRGMR
                     TELPSPLQEDVDAARADLLLAPPSAAAFVSLEAWGFDKEAERVWSHLGWRERRRASRR
                     DVDASLLTDVHAQVVREHRDGRVDILPGFRTEWLGQHLAVHDVPLHAGVLSYAIRWHG
                     ARPAVLWDAPAGVTLRAPALDPDWSSTTAVGEGLLAEPRVELLAMGKAPAHDGTPIDE
                     PESFT"
     gene            2343..>3325
                     /locus_tag="VFX21_17185"
     CDS             2343..>3325
                     /locus_tag="VFX21_17185"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5097759.1"
                     /translation="MSDVDDFMAAGLYDPEAVDAEARLALLRYLADEIGASIPEIVQT
                     HEDGGILSFAAFRTLRARGERYTLNEIAQQAGMRPDVARLFWRCVGFADPRPFERRFD
                     QRDVETLQLFRMLSELVGEQRSLSLARTFGESVARLADAEVALLRSNVEAPLAASQQY
                     AAVARAYVNIVNELVPRIATAFDALHAHHIQAIGRRYSEVGAPTSALNVVELAVAFAD
                     LSGFTGLSMQLEPAHLGEMIDRFEAVTGDTIIAAGATVAKRIGDAVMFVSNAPGVACA
                     LALDLIEGCRNAGLPKLRVGVAFGEVIVRQGDFYGGTVNLAARLVGAADAGT"
BASE COUNT          449 a         1139 c         1224 g          513 t
ORIGIN      
        1 cacgtcaagg acgcgacgat ccgcatcatg gacgcgatca cgcactgcct catgcgggcg
       61 cgcgagatct acccgcaacg acccgcgccc ggcgaggacg catggtggtg gcgcgacgcg
      121 aaggtcgcgt cgtgaaggtg gggtcgtgaa ggtcgcggtc atcggcgccg gatcgtgggg
      181 gaccaccgtc gcgatcatcg cggccgcgaa ctcgccgacg gtgttgtggg cgcgccgtcc
      241 cgacctggcc gagcgcatga cggcgacgcg cgagaacccc gactacctgc ccggcatcga
      301 gttcccggag gcgctcgcgg tcaccggttc gctcgaagcg gcgtgcgatc gcgcggacgt
      361 gatcgtgctc gcggtgccgt cgcacggctt ccgcgacgtc ctcaacggtg cacagtcgtt
      421 catcggtgtc gacgtgccgg tcatctcgct gtcgaaaggc atcgagcagg gcacgttgca
      481 gcgcatgacg gaagtggtcg gcgaagtgct cgaggggcac catccggccc gtatcggtgt
      541 gctgaccggt ccgaacctcg ccaaggaggt cgcgggcggg caaccgaccg catcggtcgt
      601 cgcgatcggc gacgaggtgg ttgccgagcg gttgcagcgc gccttcgcga cgccgacgtt
      661 ccgcgtctac acgaatcccg atgtcgtcgg ctgcgagatg gcgggcgcgt tgaagaacgt
      721 gctcgcgatc ggcgccggca tcgccgacgg cctcggctac ggcgacaaca ccaaggccgc
      781 gttgatgacg cggggcctgg cggagctggg ccggctcggt gtcgcgatgg gtggcgaccc
      841 gctgacgttc gcgggcttgg ccggcatggg cgacctcatt gcgacgtgca gcagcccgca
      901 gagccgcaac cgtcacgtcg ggttcgagct ggggcgcggc cattcgctcg acgcgatcgt
      961 gggtgagatg aacatggtgg cggagggcgt gaagagcacg gctgcggtga tcgagctcgc
     1021 ccaacgtcac gacgtcgaga tgccgctgtc ttcgttcgtc ggccgggtgc tgtacgagaa
     1081 cgcccgcccc gccgacctgg ttccggagtt gatgctgcgc aaggcgaaac ccgagttgca
     1141 cggcatgcgt tgatgacgcg gctcggcacc attggtgggg ccgcaaccgc gaccgtcgac
     1201 gcgggcggct gtgtgaccgc gggcgcgcgc accctcgact ggtggatcgg cgccgacgac
     1261 cgctggcacg aaccggcggg cgaggcgggc cggcggcagt ctcgactggg tccgggtccg
     1321 gtggtggaga cgtcgatccg ggtgccgggt ggggacgcgc accagcgcgt gtacgcggtg
     1381 accgcgcccg acgcgatcgt catggaggtc gagaacgcct caccggcgcc gttcgtgttc
     1441 accgcggtgt tgcgcgcgtc gggcacagtc gcactcgccg accgcgtgct gaccgtcgac
     1501 ggcgttcccg tgctcaccgt cccgcgcccg ccgttgcgct ggtcggtcgg gcgtctgaca
     1561 gggtcgaatt ccgaccctgt gagacgcccg accaacttgg tgcgggacga ggtgtttggc
     1621 ggcgaggcgc agacggggcc cttcgcctcg gtgacgggcg atgtcgaggt cgccttgtgt
     1681 ttctcggtgt cgcaccgcac gcggtcgcgg gtcgcgctcg cgtcggagcc ggtggatctc
     1741 gtcgcgctgg cctcacccga cgagaccgcg cgcggctggg agcaccagct cgaccggggc
     1801 atgcgcaccg agctgccgtc gccgttgcag gaggacgtcg acgcggcccg cgccgacctc
     1861 ttgctggcgc cgccgtccgc ggccgcgttc gtgtcgctgg aagcatgggg cttcgacaag
     1921 gaagccgagc gcgtgtggtc gcacctcggg tggcgcgagc ggaggcgcgc gtcgcggcgc
     1981 gacgtcgacg cgtcgctgct gaccgacgtg cacgcgcagg tcgtgcgcga gcaccgcgac
     2041 ggccgcgtcg acatcctgcc cgggttccgc accgagtggc tcgggcagca tcttgccgtg
     2101 cacgacgtgc cgctgcacgc gggcgtactg tcgtacgcga tccgctggca cggggcgcgg
     2161 cccgcggtgt tgtgggacgc gcccgccggt gtgacgttgc gcgcgcccgc gctcgatccc
     2221 gactggtcgt cgacgaccgc ggtcggcgaa ggactgctgg ccgagccgcg cgtcgagctg
     2281 ttggcgatgg gcaaggcgcc ggcgcacgac ggcacgccga tcgatgaacc cgaatccttc
     2341 acgtgagcga cgtcgacgac ttcatggccg ccgggttgta cgaccccgaa gccgtcgacg
     2401 ccgaggcccg cctcgcactc ttgcgctacc tcgccgacga gatcggcgcc tcgatccccg
     2461 agatcgtcca gacgcacgaa gatggcggca tcctcagctt cgccgcgttc cgcacgctgc
     2521 gggcccgcgg cgagcgctac acgctcaacg agatcgcgca gcaggccggc atgcgtcccg
     2581 acgtcgcccg gctgttctgg cgctgcgtcg ggttcgccga cccccgcccg ttcgagcgcc
     2641 gcttcgacca gcgtgacgtc gagacgctgc agctgttccg gatgctgtcg gagttggtcg
     2701 gtgagcagcg gtcgttgtcg ctggcccgca cgttcgggga gtcggttgcc cgactcgccg
     2761 atgccgaagt cgcgctgctg cgctcgaacg tcgaggcgcc gctcgccgca tcgcagcagt
     2821 acgcggccgt cgcccgcgcc tacgtgaaca tcgtgaacga gctcgtcccg cgtatcgcga
     2881 ccgcgttcga cgcgctgcac gcccaccaca tccaggcgat cggccgccgc tacagcgaag
     2941 tcggcgcacc gacgtcggcg ttgaacgtcg tcgagctcgc ggtcgcgttc gccgacctgt
     3001 ccgggttcac cggcctctcg atgcagctcg agccggcgca cctcggcgag atgatcgacc
     3061 ggttcgaggc ggtgacggga gacacgatca tcgccgcggg tgcgaccgtc gccaagcgca
     3121 tcggcgacgc ggtgatgttc gtctcgaacg cgcccggtgt cgcctgcgcg ctcgcgctcg
     3181 acctcatcga gggttgccgc aacgcgggcc tgccgaagtt gcgcgtcggc gtcgcattcg
     3241 gcgaagtgat cgtccgccag ggcgatttct acggcggcac cgtgaatctc gcggcccgcc
     3301 tcgtcggcgc cgccgacgcg ggcac
//
LOCUS       DASUGA010000871         2310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_74464, whole genome shotgun sequence.
ACCESSION   DASUGA010000871 DASUGA010000000
VERSION     DASUGA010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74464"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..536
                     /locus_tag="VFX21_17190"
     CDS             144..536
                     /locus_tag="VFX21_17190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097760.1"
                     /translation="MTKRKVGIFVLALVGVTIGMVSVIALREATLSTHDEVIAPDSQI
                     EVVMKANIKGEEENQTIEELVTAQVTYCRLEVESDLVGPIERREDGKFVAVLSPAMDQ
                     SNRRQFRGCLEDWGLDHLKVDVTSLVEV"
     gene            593..1234
                     /locus_tag="VFX21_17195"
     CDS             593..1234
                     /locus_tag="VFX21_17195"
                     /inference="COORDINATES: protein motif:HMM:NF021487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEX5097761.1"
                     /translation="MTALQSRELLDALKRAAAALRGAQIPFAVGGGLAAWARGGPPTE
                     HDIDLLIREDDARRALDACAAAGMRTEVPPEGWLVKAWDGEILVDLIFRPTGSIVDDG
                     LFDRCEVLNVQAVRMSVLPVDDILATKLLALTEHNLDYGPVLEYARSLREQIDWETLR
                     RRTDGSPFARAFFTLVEQLGVWHGDAARAAGAAEALHAADASAAEAADQPADG"
     gene            1227..1484
                     /locus_tag="VFX21_17200"
     CDS             1227..1484
                     /locus_tag="VFX21_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX5097762.1"
                     /translation="MADATPLDYLGAHLEEALATDGRVAEQGLHVAVDGVVLVVTGTV
                     STPERCSAIDVVARETVPDTPVRNEAVVAGVGDHAGPEDVR"
     gene            1481..2194
                     /locus_tag="VFX21_17205"
     CDS             1481..2194
                     /locus_tag="VFX21_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5097763.1"
                     /translation="MIRVAAIGDIHLGLDSRDYLRPHLEGLADDADVFVIAGDLTKCG
                     TADEARVVAAELRDVRIPCIAVLGNHDFHADEQDKVTAIMAEAGVTVLEGTSASIDVG
                     GQRLGIAGAKGFGGGFIGAMASEFGEPEMKAFVHHTKVVAQQLSTALRSLDTDVKVAV
                     THYSPVEDTLEGERREIYPFLGSYLLAEAVDGCGVVMALHGHAHAGREHGCTPGGTPV
                     RNVAQPVLGVAYKVYVVAS"
BASE COUNT          382 a          772 c          793 g          363 t
ORIGIN      
        1 cagcggctcg cggcgcagct cgggccgatc agctatccgc gtggtggtct gctcgtcgac
       61 gacatcaccg tgctcgcgca agacgtgccc ggcgcggacg acgcgcagat ccgcaagctg
      121 ttgcgccggg ccggccacgg cggatgacga agcgcaaggt cgggatcttc gtcctcgcac
      181 tcgtcggcgt gaccattggc atggtcagcg tgattgcgtt gcgcgaggcg acgctgtcga
      241 cgcacgacga agtcatcgcg cccgactcgc agatcgaagt cgtgatgaag gccaacatca
      301 aaggcgagga agagaaccag acgatcgaag agttggtcac cgcgcaggtg acctactgcc
      361 ggctcgaagt cgaatcggat ctcgtcggcc cgatcgaacg acgtgaagac ggcaagttcg
      421 tggccgtcct ctcgccggcg atggaccaat cgaaccggcg ccagttccgc ggctgcctgg
      481 aggactgggg cctcgatcat ctgaaggtcg acgtcacgag cctcgtcgag gtttagtcct
      541 tgtcgctccg ggctcgtgtc cgtgccggag cggacgggta catacacacg cgatgaccgc
      601 gttgcaatca cgagaattgc tcgacgcgtt gaaacgggcc gcggccgcgc tgcgcggtgc
      661 gcagattccg ttcgcagtcg ggggtggtct cgcggcgtgg gcgcgcggtg gcccgccgac
      721 cgagcacgac atcgacctcc tgatccggga agacgatgcg cgacgcgcgc tggacgcgtg
      781 cgcggccgcg ggcatgcgca ccgaagtgcc gccggaaggc tggttggtca aggcgtggga
      841 cggcgagatc ctcgtcgacc tgatcttccg gccgaccggc tcgatcgtcg acgacggtct
      901 gttcgaccga tgtgaggtgc tgaacgtcca ggcggtgcgc atgtctgtgc tgccggtcga
      961 cgacatcctc gcgacgaagc tcctcgcgtt gacggagcac aatctcgatt acggcccggt
     1021 gctcgagtac gcccgcagcc tccgcgaaca gatcgactgg gagacgctgc ggcgccgcac
     1081 cgacggatca ccgttcgcac gcgcgttctt cacgctcgtg gagcaactcg gcgtgtggca
     1141 cggagatgcc gcgcgcgccg ccggtgccgc ggaggcgctc cacgccgccg acgcgtccgc
     1201 cgccgaagcc gccgaccaac cggctgatgg ctgacgcaac cccgctcgac tacttgggcg
     1261 cgcatctcga ggaagcgctc gccaccgacg gccgcgtcgc cgagcaaggt ttgcacgtcg
     1321 ccgtcgacgg ggtcgtgctg gtcgtcaccg gcacggtgtc gacgcccgaa cgctgcagtg
     1381 cgatagacgt ggtcgcgcgc gagacggtgc cggacacgcc cgtccggaac gaagcagtgg
     1441 tcgccggcgt cggcgatcac gccgggccgg aggacgtgcg gtgatccgcg tcgcggccat
     1501 cggcgacatt cacctgggac tcgactcgcg cgactatctg cgcccgcatc tcgaaggcct
     1561 cgccgacgac gcggatgtct tcgtcatcgc cggcgacctg acgaagtgcg gcacggccga
     1621 cgaagcgcgc gtcgtcgccg ccgagctccg agatgttcgc atcccgtgca ttgccgtgct
     1681 cggcaatcac gacttccacg ccgacgagca agacaaggtc actgcgatca tggcggaagc
     1741 aggcgtcacc gtgctcgagg gcacgtcggc gtcgatcgac gtcggcgggc agcgactcgg
     1801 catcgcgggc gcgaagggat tcggcggcgg gttcatcggc gcgatggcgt cggagttcgg
     1861 cgagcccgag atgaaggcgt tcgtacacca caccaaagtc gttgcccaac agttgtcgac
     1921 cgcactgcga tcactggata ccgacgtcaa ggtcgcggtg acgcactact cgccggtcga
     1981 ggacacactc gaaggcgaac gccgcgagat ctatccgttc ttgggcagct acctcttggc
     2041 cgaagcagtc gacggctgcg gtgtggtcat ggcgttgcac gggcacgcgc acgccggtcg
     2101 cgagcacgga tgcacgcccg gcggaacacc ggtgcgcaac gtcgcccaac cggtgctcgg
     2161 cgtcgcgtac aaggtctacg tcgtcgcgtc ctagtgctcc gaccggcaac gttcgccgcg
     2221 ttcacgccgg ccagtgacgc cgctcgcggc gtcctcctcc tcaaaagagg cccactcttc
     2281 cttcgtcgtg cgtccttgcg agcgacgccc
//
LOCUS       DASUGA010000872         3731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_353544, whole genome shotgun sequence.
ACCESSION   DASUGA010000872 DASUGA010000000
VERSION     DASUGA010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353544"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..705)
                     /locus_tag="VFX21_17210"
     CDS             complement(88..705)
                     /locus_tag="VFX21_17210"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX5097764.1"
                     /translation="MRFRLVPRDEGFFPLFDNAAQNVAECARRLRDLLSDLPDSLEKR
                     ELVRECERLGDEITRSILHRLNSSFVTPFDREDIHTLTEEIDDVVDDMEAAADLLTLH
                     RVTNTLPEMVAMADVLVEAAEVTVALIAKLPKLRDLDVDLEAIDKLESKADSLYRQSV
                     ARLFSGEYDAFDVLKLKDIVEAIEHSVNAIENISDIVESIALKHG"
     gene            786..1979
                     /locus_tag="VFX21_17215"
     CDS             786..1979
                     /locus_tag="VFX21_17215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5097765.1"
                     /translation="MTAVLAVALVAACVVAAGALLAMRRARAEIERARVALVLDAHAE
                     VGPAVAQLKQDADDRVADAEALQHWLIGALDQSSDGIVVVDRIGREILRNAAARTFHG
                     ARHGDVLAEDAMQELFERALLGQVGERELQLYGPPRQVLHLRSFPLYASGEIVGAVTF
                     SRDVSEARRVESVRRDFVANVSHELKTPIGALGLLAETMAASDDPVVMQRLGERVSRE
                     ADRLARIVDDLLDLSLIEAQEHTIREPVPASALVGEAVDRMRAVAETAGVPLQVEPEP
                     PEVLVSCDHRQLRSALVNLLENAIKYSEPGQTVEIGAWKDDAAVAFVVRDHGIGIPTR
                     DLERIFERFYRVDRARSRDTGGTGLGLAIVRHVAQAHGGDVVVESREGEGSAFTLSIP
                     IAKAS"
     gene            1979..2692
                     /locus_tag="VFX21_17220"
     CDS             1979..2692
                     /locus_tag="VFX21_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5097766.1"
                     /translation="MPDPPLILVVDDEQSYRDALSVALQREGFLVATAADGPEAIATF
                     DASRPSLVLLDVMLPKISGIDVCRQLRAKSNVPIIMVTARTSEIDAVVGLEVGADDYV
                     SKPFRLRELIARVRVALRRAPAEEPEVERTEVLEVGDVRIDDARHEVYVNGALVAVPL
                     KEFDLLELLMANAGRVLTRETLIDRVWGPNYFGDTKTLDVHIKRLRSKIEPDPSHPVR
                     IVTVRGVGYRYERAPVAAT"
     gene            complement(2696..3019)
                     /locus_tag="VFX21_17225"
     CDS             complement(2696..3019)
                     /locus_tag="VFX21_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016878183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5097767.1"
                     /translation="MANATETCCPTVLAAPLDEHEAEDLARLFSVLGDPVRLRLLSLV
                     ADADEVCACELMEPLGRSQPTISHHTKLLAEAGLIVGEKRGRWVYWRVVPSRMRELRR
                     ALGPR"
     gene            3122..3577
                     /locus_tag="VFX21_17230"
     CDS             3122..3577
                     /locus_tag="VFX21_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020171199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HEX5097768.1"
                     /translation="MSRVQLALNVSDLDSAVAFYSKLFGSEPAKVRPGYANFAIAEPP
                     LKLVLIEGHGEGGTLNHLGVEVESTDEVGATQRRLANEGLATATEDEVACCYAVQDKV
                     WVDAPDGEPWEIYTVLSDVEMAPGELRTVEPGADAVCCATAPESAARCC"
     gene            3582..>3731
                     /locus_tag="VFX21_17235"
     CDS             3582..>3731
                     /locus_tag="VFX21_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018761658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEX5097769.1"
                     /translation="MTHDMIRRASAEFVGTAGLVAVVVGSGIAAQQLSPGNTGLQLLE
                     NSLATG"
BASE COUNT          602 a         1229 c         1273 g          627 t
ORIGIN      
        1 gagttggcgg cgtcgtggaa gccgttgacg aagtcgaagc cgagcgccac gacgaccgtg
       61 acgatgagga gcaaacctgt cacgaagtca gccgtgcttg agcgcgatgc tctcgacgat
      121 gtcggagatg ttctcgatcg cgttgacgga atgctcgatc gcttcgacga tgtccttgag
      181 cttcagcacg tcgaacgcgt cgtactcgcc cgagaacagt cgcgcgaccg attgccggta
      241 cagcgagtcg gccttgctct ccagtttgtc gatcgcctcg agatccacgt cgaggtcgcg
      301 cagcttcggc agcttggcga tgagcgcgac cgtgacctcg gccgcctcga cgagcacgtc
      361 ggccatcgcc accatctcgg gcagcgtgtt cgtcactcgg tgcagcgtga gcaggtcggc
      421 ggccgcctcc atgtcgtcga cgacgtcgtc gatctcttcg gtgagcgtgt ggatgtcctc
      481 ccggtcgaag ggagtcacga acgacgagtt gagccggtgc aggatggagc gggtgatctc
      541 gtcgccgagc cgctcgcact cgcgcacgag ctcgcgcttc tccagggaat cggggagatc
      601 ggagagcaga tcgcgaagcc ggcgagcgca ctcggcgacg ttctgggccg cgttgtcgaa
      661 gagcggaaag aaaccctcgt ctctcgggac gagcctgaaa cgcacgatct acccccgtcg
      721 ggcccggttg gagccccggc agtctgctgg atccgcctcc cgggctcaaa ctgcacgagg
      781 gtgatatgac cgccgtgctc gccgtcgcgt tggtcgcggc ctgcgtggtt gcggccgggg
      841 cccttcttgc gatgcgccgg gcccgggccg agatcgaacg ggctcgtgtt gccctcgtcc
      901 tcgatgccca tgccgaggtc ggcccggcgg tggcccaact caaacaggac gccgacgacc
      961 gggtcgcgga cgccgaggcg ctccagcact ggctcatcgg ggcactcgat caatcctcgg
     1021 acggcatcgt cgtggtcgac cgcatcggac gggagatcct gcgcaatgcc gcggcccgca
     1081 cctttcacgg cgcccggcac ggcgacgtcc tggcggagga cgcgatgcaa gagctgttcg
     1141 agcgcgcact cctcggtcag gtgggcgaac gcgagctcca gctctacggt ccaccccggc
     1201 aagtgctgca cctgcgctcg ttcccgctgt acgcgtcggg tgagatcgtc ggcgcggtga
     1261 cgttcagtcg cgacgtgtcc gaagcgcggc gtgtcgaaag cgtgcgccgc gacttcgtgg
     1321 ccaacgtgag ccacgagctc aagacgccga tcggtgcgct gggcctcctg gccgagacaa
     1381 tggcggcgag cgacgatccc gtcgtcatgc agcgactcgg tgagcgcgtc tcgcgcgaag
     1441 cagatcgcct cgcgcggatc gtcgacgatc tgctcgatct gagcctcatc gaagcccagg
     1501 agcacaccat tcgcgagccg gtgcccgcat cggcgctcgt cggcgaggcc gtcgaccgga
     1561 tgcgcgcggt cgcggagacg gccggggtgc cgctgcaggt cgaacccgag ccgcccgaag
     1621 tcctcgtcag ctgcgatcat cgccagctgc gcagcgccct cgtcaatctg ctcgagaacg
     1681 cgatcaagta ctcggagccg ggacagacgg tggagatcgg tgcctggaag gacgacgccg
     1741 cggttgcgtt cgtcgttcgc gaccacggca tcggcatccc gacgcgtgat ctcgaacgca
     1801 tcttcgaacg gttctatcgc gtcgaccgtg cgcgcagccg cgacaccggc ggcaccggtc
     1861 tcggcctcgc gatcgtccgg catgtggcgc aggcgcacgg cggcgacgtc gtcgtcgagt
     1921 ctcgcgaggg cgaaggctcg gcgttcaccc tgtcgatccc tattgcaaag gcttcttgat
     1981 gcccgacccg ccgctgattc tcgttgtcga cgacgaacag tcgtatcgcg atgcgctgtc
     2041 ggtcgcgttg cagcgcgagg gatttctcgt cgcgacggcg gcagacggac cggaggcgat
     2101 tgcgacgttc gacgccagcc gcccctcact cgtgctcctc gacgtcatgc tgccgaagat
     2161 ctccggtatc gacgtgtgcc ggcaactgcg ggcgaagtcg aacgtcccca tcatcatggt
     2221 caccgcgcgg acctcggaga tcgacgcggt ggtcggcctg gaggtcggag ccgacgacta
     2281 cgtgtccaaa ccatttcgtc tgcgcgagtt gatcgcacgg gtccgggtcg cgttgcgccg
     2341 cgcgccggcc gaggaaccgg aggtcgaacg gaccgaggtg ctcgaagtcg gcgacgtgcg
     2401 tatcgacgat gcccggcacg aggtgtacgt caacggcgcg ctcgtggccg tgcccttgaa
     2461 ggaattcgac ctgctcgagc tgttgatggc gaacgccggt cgggtgctca cccgtgagac
     2521 gttgatcgac cgggtctggg gaccgaacta cttcggcgac acgaagacgc tcgacgtgca
     2581 catcaagcgc ctccggtcga agatcgagcc cgacccttca cacccggtgc ggatcgtcac
     2641 cgtgcgcggt gtcggctatc gctacgagcg ggcgccggtc gcggcgacgt gacgcctagc
     2701 gcggcccgag cgcgcgccgc agttcgcgca tgcgactcgg gacgacgcgc cagtagaccc
     2761 accggccgcg cttctcgccg acgatcaagc ctgcttcggc gagcagcttc gtgtggtgcg
     2821 agatcgtcgg ctgtgatctg ccgagtggtt ccatgagctc gcacgcgcac acctcgtcgg
     2881 cgtcggcgac gaggctgagc agccgcagcc ggaccgggtc gcccagcacc gagaacaggc
     2941 gggcgaggtc ttcggcttcg tgctcgtcca gcggcgccgc gaggacagtc gggcagcacg
     3001 tctcggtcgc gttcgccatg ggaacctcat acattcatcg ctgtcgatct attgacaatc
     3061 ttcgatggat cgatgatgat ggatcaatcg aagattgtca atgtacagga gagtcaccgc
     3121 catgtcacgt gtccagcttg ccctcaacgt cagcgacctc gattcggcgg tcgccttcta
     3181 ctcgaagctg ttcggcagtg agccggccaa ggtccgcccc ggctacgcca acttcgcgat
     3241 cgcggagccg ccgctcaagc tcgtgctcat cgagggccac ggcgagggcg gcacgctcaa
     3301 ccacctcggc gtcgaggtcg agtccaccga cgaggtcggt gctacccagc ggcgcctcgc
     3361 gaacgagggc ttggcgaccg cgaccgaaga cgaagtcgcg tgctgctacg cagtgcaaga
     3421 caaggtgtgg gtcgacgcgc ccgacggcga accgtgggag atctacaccg tcttgtccga
     3481 cgtcgagatg gcgcccggtg aactgcgcac ggtcgagccc ggcgccgacg ccgtgtgctg
     3541 cgccaccgcg cccgagtctg cggcgcgctg ctgctgactt catgacgcac gacatgattc
     3601 ggcgcgcgag cgccgaattc gtgggaacgg ccgggctcgt cgcggtggtc gtcggttcgg
     3661 ggatcgctgc gcagcagctg tcgcccggca acaccggctt gcagctgctc gagaattcac
     3721 tcgcgacggg g
//
LOCUS       DASUGA010000873         3458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1362083, whole genome shotgun sequence.
ACCESSION   DASUGA010000873 DASUGA010000000
VERSION     DASUGA010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362083"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFX21_17240"
     CDS             complement(<1..296)
                     /locus_tag="VFX21_17240"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5097770.1"
                     /translation="MKRVHLERRDAVAIVTLDDPDRRNAMTAQMVEEIVATFDELEAD
                     GTTAAAVITGRGPAFCSGADVSNLGALAAAQSDEERRSVTSIYEGFLRVLRSSLP"
     gene            complement(321..1355)
                     /locus_tag="VFX21_17245"
     CDS             complement(321..1355)
                     /locus_tag="VFX21_17245"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5097771.1"
                     /translation="MRVLVTGLSTFWGGRVAQALERRPDVEIVVGVDTRDPRIPLERT
                     EFVRTDSTHSILSRIVRATQVDTIVHTHLIVDSTRVSGRTLHEINVIGTMNLLAAAGA
                     PSSPVRKVVLKSSGLVYGANAADPYFFREDMQRTSPPRTGVERSLLEVEAFVRDFADD
                     NPHIDVTMLRFANVLGDDIDTPFAIALRRPVVPEIMGFDPRVQFVHEDDVVDALMYAT
                     TNEIPGLYNVVGDGNLPWSEVCAMVGKRRLAVPFVLTNLASEPARLLNLWDLPPEALA
                     LLRYGRSLDNGRYKRAGYRYSFTTASTVEAFAQGLRLSGTVGDKHPSYRYEREVEDFF
                     RHSPAVVRPD"
     gene            complement(1359..2312)
                     /locus_tag="VFX21_17250"
     CDS             complement(1359..2312)
                     /locus_tag="VFX21_17250"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5097772.1"
                     /translation="MTDGPERPRPERARELGAVEIAAATHPDLDLTDLDPGVGRELVP
                     LARSQSESETGEMRRSDVDRWGRSEHFRELTRRVYEPIYSKWFRVEWEGWEHLPETGG
                     ALLVANHAGAIPPDAPSIMHGIERDLKRPVYGLAENLFRKMPVIGTLWSRSGGVAAHP
                     DNAYRLLHDEQQLVLVFPEGTKGTGKPYNERYRLRRFGRGGFVEIAMRAGVPVIPIAV
                     MGAEEAMPILFKSPRLAKAMNIPYFPITANMLVAGPLGLAAYFPAKFKIRVLPPVLFD
                     VPPNQERYSRSRVMEESERIRRLVQDALYDMLRTRQSVWFG"
     gene            complement(2322..>3458)
                     /locus_tag="VFX21_17255"
     CDS             complement(2322..>3458)
                     /locus_tag="VFX21_17255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX5097773.1"
                     /translation="FNFLAYATTATVARRVGAGDARGAAAHGVAGLWLALGLGIALML
                     FGLAVSGPAVNLMGASENVAPFARTYLRISLLGAPFVLVALAGTGYLRGLQDTRTPLF
                     IALGANALNLGLELALVYGFDLGIAGSAWGTVVAQCGAALVYVFILDRKGRRAGATRR
                     PERRQLRQAAVIGAQLTVRTGTLLIVFTTASAIAARLGDIEIAAHQIAWQLWWFAALS
                     LDAIAIAGQAIVGRSLGASDATGARRASRRMIQWGIGIGAVFGLVIIAAEPLFAVAFT
                     NDDAVRDLLFGVLLSVGIMQPLAGAVFVLDGILIGAGDSRYLAIAMTLAMLSFLPFAI
                     IVAVTNAPLVALWGALYVFMVARLYGLGRRYRRDQWLVTGALAT"
BASE COUNT          587 a         1119 c         1231 g          521 t
ORIGIN      
        1 ggcaacgacg agcggagcac gcgcaggaag ccttcgtaga tcgacgtgac cgaccgtcgt
       61 tcctcgtccg attgcgccgc cgcgagcgcg ccgagattcg acacgtcggc gcccgaacag
      121 aacgcgggcc cgcgcccggt gatgacggcc gcggccgtgg tgccgtcggc ttccagctcg
      181 tcgaaggtcg cgacaatctc ttcgaccatc tgggcggtca ttgcattgcg gcgatcggga
      241 tcgtcgaggg tcacgatcgc gaccgcgtcg cgccgctcga ggtggacacg cttcatcgct
      301 gctgctttca gtcgggtcga tcagtcaggt cgtacgacgg cgggggagtg ccggaaaaag
      361 tcttcgacct cgcgctcgta gcggtacgac ggatgcttgt cgcccacggt gcccgagagc
      421 cggagtcctt gcgcgaacgc ctcgaccgtc gacgcggttg tgaacgagta ccggtagccg
      481 gcgcgcttgt aacggccgtt gtcgagcgag cggccgtagc gcaacagtgc gagcgcttcc
      541 ggcggcaagt cccagaggtt gagcagacgc gccggttcgg acgcgaggtt ggtcagaacg
      601 aacgggacgg cgagccgccg cttgccgacc atggcgcaaa cctcgctcca cgggaggttg
      661 ccgtcaccga cgacgttgta gagaccgggg atctcgttcg tggtcgcgta catcagcgcg
      721 tcgacgacat cgtcctcgtg cacgaactgc acgcgcggat cgaagcccat gatctcgggc
      781 acgaccgggc ggcgcaacgc gatcgcgaac ggcgtgtcga tgtcgtcgcc gagcacgttc
      841 gcgaagcgca gcatcgtgac gtcgatgtgc ggattgtcat cggcaaagtc gcgcacgaac
      901 gcttcgacct cgagcaacga gcgttccacg ccggtgcgag gcgggctcgt gcgctgcatg
      961 tcttcccgga agaagtacgg gtcggccgca ttcgcgccgt agacgagacc ggaactcttg
     1021 agcaccacct tgcgcaccgg actcgacggc gcaccggccg ccgcgagcaa gttcatcgtg
     1081 ccgatgacgt tgatctcgtg cagcgttcgg ccgctgacgc gcgtggagtc gacgatgaga
     1141 tgcgtgtgca cgatcgtgtc gacctgtgtg gcgcgcacaa tgcgggaaag gatcgagtgc
     1201 gtcgagtcgg tgcgtacgaa ctcggtgcgt tcgagcggga tgcgcggatc gcgcgtgtcg
     1261 acaccgacga cgatctcgac gtcgggccgg cgttcaagtg cctgcgcgac gcgaccgccc
     1321 cagaaggtcg acaggccggt gacgagaacg cgcacggctt atccgaacca cacgctctgg
     1381 cgcgtgcgca gcatgtcgta gagcgcatcc tgcacgaggc ggcggatgcg ttccgactcc
     1441 tccatcacgc ggctgcgtga gtaccgctcc tggttgggcg gtacgtcgaa caaaacgggc
     1501 ggcaggacac ggatcttgaa cttggccgga aagtacgccg ccagtccgag cggtccggcg
     1561 acgagcatgt tggccgtgat cgggaagtac gggatgttca tggccttcgc gaggcgcggg
     1621 ctcttgaaca ggatcggcat tgcctcttcg gcgcccatga cggcgatcgg aatcaccggc
     1681 acgccggcgc gcatcgcgat ctcgacgaag ccgccccgtc cgaaccggcg caggcggtag
     1741 cgctcgttgt acggcttgcc ggttcccttg gtgccttcgg ggaaaacgag cacgagttgc
     1801 tgttcgtcgt gcagcagccg gtacgcattg tcggggtggg cggcgacgcc gccgctgcgc
     1861 gaccacaacg tgccgatcac cggcatcttg cggaagaggt tctcggccag gccgtagacg
     1921 ggccgcttca agtcgcgctc gatgccgtgc atgatcgacg gcgcgtcggg cggaatcgcg
     1981 cccgcgtggt tcgcgacgag cagcgcgccg cccgtttccg gcaaatgttc ccagccttcc
     2041 cactcgaccc ggaaccactt gctgtagatg ggctcgtaca cgcgacgcgt cagctcgcgg
     2101 aaatgttcgc tgcggcccca gcggtcgaca tccgaccgcc gcatctcgcc ggtttcgctc
     2161 tcggattgac tgcgcgcaag cggaacgagc tcccggccga cgccgggatc gaggtcggtc
     2221 aggtcgaggt cggggtgcgt cgccgccgcg atctcgaccg cgcccaactc gcgtgcccgt
     2281 tcggggcggg gccgctcggg tccgtcagtc accgggcaag cctacgtagc gagggcgccc
     2341 gtcaccaacc actgatcgcg acggtagcgg cggccgagtc cgtacagccg agcgaccatg
     2401 aagacgtaca acgcgcccca caacgcgacg aggggtgcgt tggtgacggc aacgatgatg
     2461 gcgaacggca ggaagctgag cattgccagc gtcatcgcga tcgcgaggta gcggctgtcg
     2521 cccgcgccga tgaggatgcc gtcgagcacg aacactgcgc ccgcgagcgg ttgcatgatg
     2581 ccgacggaga gcaacacgcc gaaaagcagg tcgcgcaccg catcatcgtt ggtgaacgcg
     2641 acggcgaaga gcggctcggc cgcgatgatg accagcccga acacggcgcc gatgccgata
     2701 ccccactgga tcatgcggcg cgacgcgcgc cgcgcgccgg tcgcgtccga tgcgccgagc
     2761 gaccggccga cgattgcttg tccggcaatc gcgatcgcgt cgagtgagag tgccgcgaac
     2821 caccagagct gccacgcgat ctgatgggcc gcgatctcga tgtcgccgag gcgggccgcg
     2881 atcgcgctcg cggtcgtgaa cacgatgagc agtgtgccgg tccgtaccgt cagttgggcg
     2941 ccgatgaccg ctgcctggcg gagttgtcgc cgctcggggc ggcgggtcgc gcccgcgcgg
     3001 cgccccttgc gatcgaggat gaagacgtag acgagcgcgg cgccgcactg cgcgacgacg
     3061 gtgccccacg ccgagccggc gatgccgagg tcgaacccgt acaccagggc gagttcgagc
     3121 ccgaggttga gcgcgttggc gccgagcgcg atgaagagtg gtgtgcgcgt gtcttggagt
     3181 ccgcgcaggt atccggtgcc ggccagcgcg acgagcacga acggggcgcc gagcagcgag
     3241 atccgcaaat aggtgcgggc gaacggcgcg acgttttcgg atgcgcccat gagattgacc
     3301 gccggaccgc tcacggccaa gccgaacagc atgagcgcga tgccgagtcc aagcgcgagc
     3361 cagaggcccg caacgccgtg cgcggccgcg ccgcgcgcgt cgccggcccc gacccggcgg
     3421 gcgacggtcg cggtggtggc gtaagcgagg aagttgaa
//
LOCUS       DASUGA010000874         3113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_283305, whole genome shotgun sequence.
ACCESSION   DASUGA010000874 DASUGA010000000
VERSION     DASUGA010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283305"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1353)
                     /gene="purD"
                     /locus_tag="VFX21_17260"
     CDS             complement(73..1353)
                     /gene="purD"
                     /locus_tag="VFX21_17260"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX5097774.1"
                     /translation="MTRVLIVGSGGREHALAWGLARSSSVDEVMCAPGNAGMAALGEC
                     LPVVATDPDAVADLARKVDADLVVVGPEDPLAAGVVDAVEATGRPAFGPREAAAKLEG
                     SKRWMKEVLVAAGVPTARHETFTAGGEQRAFAYLETLPGLYVVKTDGLAAGKGVVVTE
                     DLAVARDAVRDYLSGAAFGDAGRTCVIEEGLIGPELSIFALCDGMDATVIGVAQDHKR
                     AFDNDEGANTGGMGAYSPVPFVGPDMVDDVMTTAIVPTLGELSARGAEFRGVLYCGLM
                     ITAAGPKVLEYNVRFGDPEAQVLVPRFRSDLYAHLLESATGKLVTKPEFDDGACVGVV
                     LASEGYPPAPLRKGDPITGLDAAGTHAGVAVFHSATATGADGEIVTNGGRVLTVSAVG
                     SDVRAARDRAYAATAEISWAGVHYRRDIAAQALT"
     gene            complement(1353..2645)
                     /locus_tag="VFX21_17265"
     CDS             complement(1353..2645)
                     /locus_tag="VFX21_17265"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX5097775.1"
                     /translation="MPGAVIVGTQWGDEGKGRVTDYLAKESSMCVRYQGGHNAGHTLV
                     VDGETFKLQLVPSGVLYPWVTPVIGNGVVVDPAVLIDEMDMLESKGVDTKPLRLSGNA
                     HLIMPYHQELDRVTERYLGKNKLGTTKRGIGPAYADKALRVGIRVQDLLDPKIFREKL
                     DLVLREKNGVLAKVYNRLPIDAKELAEQYLAMAPRLSPHIDDTVHLVHEALDAQQWVL
                     FEGAQATYLDLDHGTYPFVTSSNPVAGGVCTGAGVGPLAISRVVGLVKAYQTRVGSGP
                     IPTELPEGDEVGDLLVTRGREFGTNTGRRRRTGWLDLVMLKHAVRLNSCTELALTKLD
                     ILSPLEELKVCVGYVGDDGTRYDHVPYHQSVIHKVTPVYETLPGWGVEIERAGRIEEL
                     PPEARAYVKFVEDFAGVHVGIVGVGPARDQTIVAPRAA"
     gene            complement(2668..>3113)
                     /locus_tag="VFX21_17270"
     CDS             complement(2668..>3113)
                     /locus_tag="VFX21_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097776.1"
                     /translation="IATVAALPPDGHPPALARREWINVGLVSLISQGVQILLVSVLIG
                     AFFVILGVLLVTEKTTLTWARDVNVLLEFTVGNRNLVVTEQLLRVAGFLTAFSGLNFT
                     VYLATDETYRREFRSEVLLDLRQAFAVRAAYLGYLAGRSSTISPT"
BASE COUNT          510 a         1068 c         1043 g          492 t
ORIGIN      
        1 cctcgagcca ggcgccgaac cgcgcgtcgt cggtgaagag gtcggcgatc tcgggccggg
       61 agtaccgagg gatcacgtga gggcctgggc tgcgatatcc cgacggtagt ggacccccgc
      121 ccacgaaatc tcggccgtcg cggcgtaggc gcggtcgcgt gcggcgcgca catccgagcc
      181 gaccgccgac acggtgagca cgcgcccgcc gttcgtcacg atctctccgt cggcgccggt
      241 tgcagtggcg gagtggaaca ctgccacgcc cgcgtgcgtg ccggccgcgt cgaggccggt
      301 gatgggatcc cccttgcgca gcggcgccgg cgggtaaccc tcggaagcga gcaccactcc
      361 gacgcaggcg ccgtcgtcga actcgggctt ggtgaccaac ttgccggtcg cggactcgag
      421 caagtgcgcg tacagatcgc tgcgaaagcg cggaacgagc acctgcgcct cggggtcgcc
      481 gaagcgcacg ttgtactcga gcaccttcgg acccgcagcc gtgatcatga gtccgcagta
      541 gaggacgccg cggaactcgg cgccgcgcgc cgagagctca cccagggtcg ggacgatcgc
      601 cgtcgtcatg acgtcgtcga ccatgtccgg accgacgaac ggcaccggcg agtacgcacc
      661 catgcctccc gtgttcgcac cttcgtcgtt gtcgaacgcg cgcttgtgat cttgggcaac
      721 accgatgacc gtcgcgtcca tgccgtcgca cagcgcgaag atcgagagtt cggggccgat
      781 gaggccttct tcgatgacgc acgtgcggcc ggcgtctccg aacgcggcgc cggagaggta
      841 gtcgcgcacg gcgtcgcgcg cgaccgcgag atcctcggtg acaacgacac ccttccccgc
      901 cgccagcccg tcggtcttca cgacgtacag accgggcagg gtttcgaggt acgcgaacgc
      961 gcgctgttcg ccgccggccg tgaaggtctc gtggcgcgcg gtcggaaccc cggccgccac
     1021 gagcacttcc ttcatccatc gcttcgaacc ttcgagcttc gccgccgcct cacgcggacc
     1081 aaaggccggc cgcccggttg cttcgaccgc gtcgaccacg cccgcggcga gcgggtcctc
     1141 cggcccgacg accacgaggt cggcgtcgac cttgcgggcg agatcggcga ccgcgtcggg
     1201 atcggtcgca acgaccggca agcactcgcc gagcgcggcc atgcccgcgt tgccgggcgc
     1261 gcacatcacc tcgtcgaccg acgacgaacg cgcaaggccc cacgcgagcg cgtgttcgcg
     1321 tcccccgctt ccaacgatca agacccgcgt catcaggcgg cccggggagc aacgatggtt
     1381 tggtcgcgag cggggccgac gccgacgatg cccacgtgca cgcccgcgaa gtcttcgacg
     1441 aacttcacgt acgcgcgggc ctcgggcggc aactcttcga tgcggccggc ccgttcgatc
     1501 tcgacgcccc agcccgggag cgtttcgtag accggcgtca ctttgtggat gacagactgg
     1561 tgatacggca cgtggtcgta acgcgtgccg tcgtcgccga cgtaaccgac acacaccttc
     1621 agttcctcga gcggcgaaag gatgtcgagc ttcgtcagcg cgagctccgt gcacgagttc
     1681 aaccgcaccg cgtgcttcaa catgacgaga tcgagccaac cggtgcggcg acggcgcccg
     1741 gtattcgtcc cgaactcgcg cccgcgcgtc acgagcaggt cgccgacctc gtcgccctcg
     1801 ggcaactcgg tcgggatcgg gccgctgccc acgcgcgttt ggtatgcctt cacaaggccg
     1861 acgacccgcg agatcgcaag cggacccacg cccgcgcccg tgcacacgcc gcccgcgacg
     1921 gggttgctcg aggtcacgaa cggatatgtg ccgtggtcca ggtcgagata cgtcgcttgt
     1981 gcgccttcga acaggaccca ctgctgcgcg tcgagcgctt cgtgcacgag atgcacggtg
     2041 tcgtcgatgt gcggtgacaa ccgcggcgcc atcgcgaggt attgctccgc gagttccttc
     2101 gcgtcgatgg gcaggcggtt gtacaccttg gccaacacgc cgttcttctc gcggagcacg
     2161 aggtcgagct tctcgcgaaa gatcttgggg tcgagcaggt cttgcacccg aatgccgacc
     2221 cgcagcgcct tgtcggcgta cgccgggccg atgccacgct tggtcgtgcc gagcttgttc
     2281 ttgccgaggt agcgctcggt cacccggtcg agctcctggt ggtacggcat gatcaggtgg
     2341 gcgttgcccg agaggcgcag cggcttcgtg tcgactccct tgctttcgag catgtccatc
     2401 tcgtcgatca gcaccgccgg atcgacgacg acgccgttgc cgatgaccgg cgtgacccag
     2461 gggtacagca cgccgctcgg cacgagctgc agcttgaagg tctcgccgtc gaccacgagg
     2521 gtgtggcccg cgttgtggcc gccttgatag cggacacaca tcgaggattc ttttgcgagg
     2581 tagtcggtga cgcggccttt gccctcgtcg ccccactggg taccgacgat tacggcgccg
     2641 ggcacgaaac gctccgcctg ttcggggtca cgttggggaa atagtactcg accggcccgc
     2701 caggtatccg aggtaggccg cgcgtacggc gaatgcctgg cgcaggtcga ggagcacctc
     2761 ggaccggaac tcacgccggt atgtctcgtc agtggcgagg tacacggtga agttgaggcc
     2821 cgagaaggcc gtgaggaatc cggccacgcg caacagctgc tcggtgacaa cgagatttcg
     2881 gttcccgacc gtgaactcga gcagcacgtt cacgtcacga gcccacgtca gcgtcgtctt
     2941 ttccgtgacg agcaggacgc cgaggatgac gaagaacgcc ccgatgagca cgctgaccaa
     3001 cagaatctgc acaccctggc tgatcaggga cacgaggccc acgttgatcc attcgcgacg
     3061 ggccagcgca ggcggatggc cgtcgggcgg cagtgcggcc acggtcgcga tcg
//
LOCUS       DASUGA010000875         3133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_216130, whole genome shotgun sequence.
ACCESSION   DASUGA010000875 DASUGA010000000
VERSION     DASUGA010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216130"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..899
                     /locus_tag="VFX21_17275"
     CDS             60..899
                     /locus_tag="VFX21_17275"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5097777.1"
                     /translation="MALVIGIVVVVLFAVAFLFFTANRRRSTGALSRETRASDHSTAG
                     EVAAAASPSTDLEATGRERADETREMAGDVPAVRKSGEVVAWEPVDEEELGISRRQFL
                     NRGLLTATGFSAGALGIGVLGFLWPLKGGGFGGKVKAGSYEDFVTYVSENREPLYVPD
                     ARAYLRPYPPTALPAAKKVYSPVILAGMEEGLVALWQRCVHLGCRVPWCQSSQWFECP
                     CHGSKYNAVGEKRDGPAPRGLDRFPVTVDGGDIVIDTSTVVEGPPIGTNTTHQTQEGP
                     ACV"
     gene            899..2422
                     /locus_tag="VFX21_17280"
     CDS             899..2422
                     /locus_tag="VFX21_17280"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5097778.1"
                     /translation="MTAFVWWRADLRHFIALFNIVVILGIAGYLLWATFSKRKLDEKA
                     PPNRVAFFEDDTLEGRRLERVLGWALIFVTAFAIVFLVYLLREPVRQEHSEAYFDNAS
                     EERGEELFANSQMDAYNPVKSLSCADCHGQTGAGGGKAVILDPDGPNGPEPPQAHVWK
                     VPPLNTVLLRFQEDPECADPALKPRFVCEVSQIITYGRPGTPMPAFGVAGGGAKNDQA
                     INDIIAYLRTIQLSSDDAKKQASDQLEEARGQAAAQVEDAQTAVDDANKALDDARAAA
                     VEQLGVPATTTDDALLSDCNDLEETAKADPSKVTPEMQEQAKACRDFKAAVEARDTAD
                     ANLAWSHEWEARRANTTDGQYLFELFCARCHTEGWSVFDSATPEGVENLGLAGGGGGQ
                     GGGIGFNLRDGGTERRFGPGDIGTRSMLDFVSLGSSPFVGYGKSGIGSGRMPGFAKML
                     TADQICLIVEYERNGLQGTTYGPEAKGAEPLGANECQLPPETTTTSTAGTTTTTTGR"
     gene            2419..>3133
                     /locus_tag="VFX21_17285"
     CDS             2419..>3133
                     /locus_tag="VFX21_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097779.1"
                     /translation="MNMQLALLLAQEGGEKTLWDPTILGVITVLSAIGLFCGSVYLLL
                     STNLGARLGFLVAGACLSAFMVLLSGSWIITATPLNSPKGRPPAWHVREVVNDPSEAE
                     IDAVRPIVGNGNPQDEEQLGQLRPAIEAALVTKTVPANEEAPSQPLAQFSKTTDLLTD
                     FEGFQAYTMGGGTKNLFWHNNKYAAVQFCTRLQVEVSAGEAPPAPECDPLVSKQWAIM
                     EFDFGSIRQPPWVYFGFSVV"
BASE COUNT          516 a         1080 c         1046 g          491 t
ORIGIN      
        1 cttcaacttc gtcttcccgt ggaacgagca catcttcttc gagctgtagg gagttctcgt
       61 tggctcttgt catcgggatc gtcgtcgtcg tcctgtttgc ggtggcgttc ctcttcttca
      121 ccgccaaccg gcggcgctcg acgggtgcgt tgtcgcgcga gacgcgcgcg agcgaccatt
      181 cgaccgccgg tgaggtcgcg gcggcggcat cgccttccac cgacctcgag gccaccggcc
      241 gcgagcgcgc cgacgagaca cgcgagatgg ccggcgacgt ccccgccgtg cgcaagtcgg
      301 gcgaggtcgt cgcctgggaa ccggtcgacg aggaagagct cggcatctcg cgccgccagt
      361 tcctcaaccg cggcttgctg accgccactg gtttctcggc cggcgcactc ggcatcggcg
      421 tgctcggttt cctgtggccg ctcaagggtg gcggcttcgg cggcaaggtg aaggccggtt
      481 cgtacgagga cttcgtcacc tacgtcagcg agaaccgcga gccgctgtac gtccccgacg
      541 cccgcgcgta tctgcgcccg tacccgccga ccgcgctgcc cgcggcgaag aaggtgtact
      601 cgccggtgat cctggccggt atggaagagg gcctcgttgc gctgtggcaa cgctgcgtgc
      661 acctcgggtg ccgcgtgccg tggtgccaga gctcgcagtg gttcgagtgt ccctgccacg
      721 gctcgaagta caacgccgtc ggcgagaagc gcgacggtcc cgcgccgcgt ggtctcgacc
      781 ggttcccggt gaccgtcgac ggtggtgaca tcgtcatcga caccagcacc gtggtcgaag
      841 gtccgcccat cggcacgaac acgacccacc agacgcagga aggaccggcg tgcgtctgat
      901 gactgcgttc gtatggtggc gggcagacct tcgtcacttc atcgccctgt tcaacatcgt
      961 cgtcatcctc gggatcgccg gctacctgct gtgggccacg ttctcgaagc gcaagctcga
     1021 cgagaaggcg cccccgaacc gcgtcgcgtt cttcgaggac gacaccctcg aagggcggcg
     1081 cctcgagcgc gtgctcgggt gggcgctcat cttcgtcacc gcgttcgcga tcgtgttcct
     1141 cgtgtacctg ctccgggagc cggtgcgtca ggagcactcc gaggcgtact tcgacaacgc
     1201 gtcggaggag cgcggcgaag agctcttcgc caactcgcag atggacgcgt acaacccggt
     1261 caagtcgctg tcgtgcgccg actgccacgg ccagaccggt gcgggcggcg gcaaggccgt
     1321 catcctcgac cccgacgggc ccaacggacc ggagcccccg caggcgcacg tctggaaggt
     1381 gccgccgctc aacacggtgc tgttgcgctt ccaggaagac cccgagtgcg cggatccggc
     1441 gttgaagccg cgtttcgtgt gcgaggtgag ccagatcatc acgtacggcc ggccgggcac
     1501 gccgatgccg gcgttcggtg tcgccggtgg cggtgcgaag aatgaccagg cgatcaacga
     1561 catcatcgcc tacctccgca ccatccagct gtcgtccgac gacgccaaga agcaagcgtc
     1621 cgatcagctc gaggaggcac ggggccaagc cgccgcgcag gtcgaggacg cacagacggc
     1681 ggtcgacgac gcgaacaagg cgctcgacga tgcccgcgcg gccgccgtcg aacaactcgg
     1741 cgtgcccgcg acgacaaccg acgacgcgtt gctgtccgac tgcaacgatc tcgaagagac
     1801 cgcgaaagcg gatccgtcga aggtcacgcc ggagatgcag gagcaggcca aggcgtgccg
     1861 cgacttcaag gcggccgtcg aagcgcgcga caccgcggac gccaacctcg cctggtcgca
     1921 cgaatgggag gcgcggcgcg ccaacacgac cgacgggcag tacctgttcg agctcttctg
     1981 tgcgcgctgc cacaccgaag gctggtcggt cttcgactcg gccactcctg aaggcgtcga
     2041 gaacctcggc ctcgccggtg gcggtggcgg tcaaggcggc ggcatcggct tcaacctgcg
     2101 cgacggcggt acggaacgcc gcttcgggcc gggcgacatc ggcacgcgct cgatgctcga
     2161 cttcgtcagc ctcggctcga gcccgttcgt cggctacggc aagagcggca tcggctccgg
     2221 ccgcatgccc ggcttcgcca agatgctgac cgccgaccag atctgcctga tcgtcgagta
     2281 cgaacgcaac ggtctccagg ggacgaccta cgggccggag gccaagggcg ccgaaccgct
     2341 cggtgcgaac gagtgccaac tgccgccgga gacgacgacg accagcactg ccggcaccac
     2401 gaccaccacg acggggcgat gaacatgcag ctcgcgttgc tcctcgcgca agaaggcgga
     2461 gaaaagaccc tctgggaccc gacgatcctc ggcgtcatca ccgtgttgtc cgcgatcggg
     2521 ttgttctgcg gctcggtgta cttgctgctg tcgacgaacc tcggcgcccg cctcggcttc
     2581 ctcgtcgccg gcgcctgcct gagcgcgttc atggtgttgc tctcgggctc gtggatcatc
     2641 accgcgacac cgctgaacag cccgaagggt cgtccgccag cgtggcacgt cagggaggtc
     2701 gtcaacgatc cgtcggaggc cgagatcgac gccgtgcgcc ccatcgtcgg caacggcaac
     2761 ccgcaggacg aggagcagct cggccagctc cggcccgcga tcgaggccgc gctcgtcacc
     2821 aagacggttc ccgccaacga agaggcgccc tcgcagccgc tcgcgcagtt ctcgaagacg
     2881 accgacctgc tcaccgactt cgagggcttc caggcgtaca cgatgggcgg cggcaccaag
     2941 aacctgttct ggcacaacaa caagtacgcg gcggtgcagt tctgcaccag gctccaggtc
     3001 gaagtgtccg cgggcgaggc gcccccggca ccggagtgcg acccgctcgt ctcgaagcag
     3061 tgggcgatca tggagttcga cttcggatcg atccgtcagc caccctgggt ctacttcggg
     3121 ttctccgtcg tga
//
LOCUS       DASUGA010000876         5098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1362364, whole genome shotgun sequence.
ACCESSION   DASUGA010000876 DASUGA010000000
VERSION     DASUGA010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362364"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="VFX21_17290"
     CDS             complement(<1..1082)
                     /locus_tag="VFX21_17290"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5097780.1"
                     /translation="MAVIAVVAAVDLASDDVFLTAAVIAPFIAALGASTAAVAVVAIL
                     ATGVSVALGVPNDIFFDRDHVVRVLIVAVGGIVAVVVARVRERRELELAVVGPEAQRM
                     RLALDAGEMGTWRWDLARGHVTWDARLEALFGLAPGAFNGSFDTYISMLHPDDRELAL
                     AAVQYGMQHGTPWQFEHRVVWPDGSVHWLEGRGEPVRNDSASIVGATGVTVNIDTRRS
                     MELQRSALLDAERAAREAAERSTGTLGRLAVLTAALSAAATVDDVAATMVHHGRDALE
                     SDFGWFGLVDAATDSLVARAHEGYDPPMLASYDVIPLDASVPATAALHTGDAIFVSSP
                     ADRDAHFPQFDHRLTHAAFIVLPISI"
     gene            1610..4429
                     /locus_tag="VFX21_17295"
     CDS             1610..4429
                     /locus_tag="VFX21_17295"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEX5097781.1"
                     /translation="MVAVDSFGARSQLSVGDATYNVVRLDKVVDDPRRLPYATRILLE
                     NLVRREDGVNVTGDDISALAARAGAPSNEPLELAFMPARVLMQDFTGVPAIVDLAAMR
                     DALAERGGDARRVDPAVPVDLVIDHSIQVDVFGVPDALARNAAFEYERNRERYTFLRW
                     AQQAFSTLRVFPPNQGICHQVNLEYLAQVVFRDREGNAYPDTLVGTDSHTPMVNGLGV
                     VGWGVGGIEAEAAMLGQPLSMLAPLVVGIEVQGALPEGATATDLVLTVAELLRAHGVV
                     GKFVEFYGDGVARVPVENRATIGNMSPEYGSTITIFPVDDETLRYLRATGRPDDVVAL
                     VEAYAKEQGLWHEASSRPAYDEHLVLDLSTVEPSIAGPARPQDRVPLSRAKVAFEESL
                     LAYRRQSMDSTAAPIGVEPGTLEEALDESFPASDPPAATESVSGIGAPDVNHGPGARE
                     HRVTVGRKRCPVTLADGREFELADGHVVIAAITSCTNTSNQSVMIAAGLLAQRAVERG
                     LTVPPWVKTSLAPGSLVVTDYYERAGLLAPLSKLGFDVVGYGCTTCIGNSGPLAPEIS
                     AAVSSGDLSVCSVLSGNRNFEGRIHPDCRMNYLASPPLVVAYALAGTMDHDLRNDPLG
                     EDTNGTPVYLRDLWPSGAEIAQVVRSELDTQMYRDRYATVLDGDERWRSLPAPTGDRF
                     DWDPASTYIRRPPFLDETGRVMTEPVDGVRALALLGDSVTTDHISPAGNIRRGTPAAA
                     WLEEHGVEPRDFNSYGARRGNHEVMMRGTFANVRLRNRLAPGTEGGVTLHLPDREETT
                     IYDAAMHYARDGVPLLVLAGKEYGSGSSRDWAAKGSLLLGLRAVIAVSFERIHRSNLV
                     GMGVLPLELADGATVESLGLTGHETFRITGLDALDGDGPLPFSVTVAADDRTFRTTAR
                     VDTPFEREVFLAGGILPFTLRRLLT"
     gene            4483..4821
                     /locus_tag="VFX21_17300"
     CDS             4483..4821
                     /locus_tag="VFX21_17300"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5097782.1"
                     /translation="MRLDVEIEDNDDERVARLEGMVDVFTAGGVVRRVLAGLPREATR
                     VVIDLRGVRFMDSAGLSAVVRLSERARRRALDLRAELGDASRLNPTVAGMLRRVVPCD
                     EDKHARPAAS"
BASE COUNT          810 a         1744 c         1764 g          780 t
ORIGIN      
        1 tcgatcgaga tgggcaacac gatgaacgcc gcgtgcgtca gccgatggtc gaactggggg
       61 aaatgcgcgt cgcggtctgc gggtgaagac acgaagatcg cgtcaccggt gtgcaacgcg
      121 gcagtcgccg gcaccgaggc gtcgagggga atgacgtcgt aggacgcgag catcggcggg
      181 tcgtacccct cgtgggcgcg ggccacgagg ctgtccgtgg ctgcgtcgac gaggccgaac
      241 cagccgaagt cggactccag cgcgtcgcgg ccgtggtgga ccatcgtcgc cgcgacgtcg
      301 tcgacggtcg cggcggcgga caaggcggcg gtcaacaccg cgagccggcc gagcgtcccg
      361 gtcgatcgtt ccgcggcctc acgcgcggcg cgctccgcgt cgaggagggc cgagcgctgt
      421 aactccatgg agcggcgcgt gtcgatgttg acggtgacac ctgtcgcgcc gacgatcgac
      481 gcactgtcgt tgcgcacggg ttcgccccgt ccttcgagcc agtgcaccga tccgtcgggc
      541 cacaccacgc gatgctcgaa ctgccacggt gtgccgtgct gcatgccgta ctgcaccgcc
      601 gcgagcgcaa gctcacgatc gtcgggatgc agcatcgaga tataggtgtc gaagctgccg
      661 ttgaatgcgc ccggcgcgag gccgaagagc gcttcgagcc tcgcgtccca ggtgacgtga
      721 ccgcgcgcga gatcccagcg ccacgtcccc atctcgcccg cgtcgagcgc gagccgcatc
      781 cgctgggctt cgggaccgac gacggcgagc tccagctcgc ggcgctcacg gacgcgcgcc
      841 acgacgacgg cgacgatgcc accgaccgcg acgatcagga cgcgtacgac gtggtcgcgg
      901 tcgaagaaga tgtcgttcgg cacgccgagc gcgaccgaca cgcccgtcgc gaggatcgcg
      961 accaccgcga ccgcggcggt cgaagcgccg agcgcggcga tgaagggagc aatgactgcg
     1021 gcagtcagga acacgtcgtc gctggcgaga tcgaccgcgg cgacgactgc gatgaccgcc
     1081 acccccgcaa ttgcccacga gcggtggccc cgacgcgaac gagccggagg agacacgatg
     1141 tccgcagact agttctgtcc ggcggtgcgt aaatcggacg attcagggat cctgcaactc
     1201 ggtacatctt cattgtgacg tcgatcgtcc tgccggctga gaggtcggcg gcgtccaggg
     1261 cgcggcattt cacgcgcgat gtgctggcgc gactcgatgt tccgtcggcg gtgatcgacg
     1321 atgccgaact gctcgtgtcc gaactcgtca ccaacgcggt gctgcacgcc cgatcggtga
     1381 gccgggcacg ccgacggtcg cgtacgagtc gcggtcggcg acgacagttc cgagcaaccg
     1441 cgaccgcggg gcaacggcgc ggactccgcg accggccggg gcttgttgtt ggtcgaccgg
     1501 ctcgcctgca attggggcat cgatcggtcc gacggcaccg gcaaggtcgt gtggttcgag
     1561 ctcgacacac acgaggcccg gcgcgtcgaa ggcaagcact agcgtcgaca tcgtggccgt
     1621 cgattccttt ggtgcccgtt cgcagctctc ggtcggcgac gcgacgtaca acgtcgtgcg
     1681 cctcgacaag gtcgtcgacg atccgagacg cctgccctac gcgacgcgca tcctcctgga
     1741 gaacttggtg cgacgcgagg acggtgtcaa cgtcaccgga gacgacatca gcgcgctcgc
     1801 ggcgcgcgcc ggagccccga gcaacgagcc gctggagctc gcgttcatgc cggcgcgggt
     1861 gctcatgcag gacttcacgg gcgtgcccgc gatcgtcgac ctggccgcga tgcgcgatgc
     1921 gctcgcggaa cgcggtggcg atgcacggcg cgtcgatccc gcagttccgg tcgacctcgt
     1981 catcgaccac tcgatccagg tcgacgtctt cggtgtgccc gatgccctcg cgcgcaacgc
     2041 cgcgttcgag tacgaacgta accgtgagcg ctacacgttt ctgcggtggg cgcaacaggc
     2101 attcagcacg ctgcgcgtct tcccgccgaa ccagggcatc tgccaccagg tgaacctcga
     2161 gtacctcgcg caggtcgtct ttcgcgaccg cgagggcaac gcgtatcccg acacgctggt
     2221 ggggaccgac tcgcacacac cgatggtcaa cgggctcggc gtcgtcggtt ggggcgtcgg
     2281 cggcatcgaa gccgaggccg cgatgctcgg gcagccgctg tcgatgctcg cgccgttggt
     2341 cgtgggcatc gaggtgcagg gcgcactccc cgagggcgcc accgccacgg atctcgtcct
     2401 cacggtggcc gagctgttgc gggcgcacgg cgtcgtgggc aagttcgtcg agttctacgg
     2461 cgacggtgtg gcgcgcgtcc cggtcgagaa ccgagcgacc atcggcaaca tgtcgccgga
     2521 gtacggctcg acgatcacga tcttccccgt cgacgacgag acgctgcgct atctgcgcgc
     2581 gacgggccgg cccgacgacg tcgtcgcgtt ggtcgaggcg tacgcgaagg aacaagggct
     2641 ctggcacgaa gcctcgtcgc gccccgcata cgacgaacac ctcgtgctcg atctgtcgac
     2701 ggtcgagccg tcgatcgcgg gtccggcgcg accacaggat cgcgtgccgc tgtcgcgggc
     2761 gaaggtggca ttcgaggagt cactgctcgc ataccgccgc cagtcgatgg attcgacggc
     2821 ggcaccgatc ggtgtcgagc ccggcacgct cgaagaagcg ctcgacgagt cgttccccgc
     2881 gagcgatccg ccggccgcga ccgagtcggt ctccggcatc ggcgcacccg acgtcaacca
     2941 cggtccgggc gcccgtgagc atcgggtcac ggtcggccgc aagcggtgcc cggtcacgct
     3001 cgccgacggc cgcgagttcg agctcgcgga cggtcacgtc gtgatcgcgg cgatcacgtc
     3061 gtgcacgaac acgtcgaacc agtcggtgat gatcgccgcc ggtctgctcg cgcaacgcgc
     3121 ggtcgaacgt ggtctgacgg tcccgccgtg ggtgaagaca tcgctcgcgc cgggctcgct
     3181 cgtcgtgacc gactactacg aacgggccgg tctgctcgcg ccgttgtcga agctcggctt
     3241 cgacgtcgtc gggtacggct gcacgacgtg catcggcaac tcgggcccgc tcgcgccgga
     3301 gatctcggcc gcagtatcga gcggcgatct ctcggtgtgc tcggtgctgt ccggcaaccg
     3361 caacttcgag ggccgcatcc atcccgactg ccgcatgaac tatctcgcgt cgccgccgct
     3421 cgtcgtcgcc tacgcgttgg cgggcaccat ggaccacgat ctccgcaacg accccctcgg
     3481 cgaggacacg aacggcacgc cggtgtactt gcgcgacctc tggccgagcg gagccgagat
     3541 cgcgcaggtc gtccggtccg agctcgacac ccagatgtac cgcgaccgtt acgcgaccgt
     3601 gctcgacggc gacgaacgct ggcgcagcct gcccgcaccg accggtgatc gcttcgactg
     3661 ggacccggca tccacctaca tccggcgtcc accgttcctc gacgagaccg gtcgggtgat
     3721 gaccgaaccc gtcgacggtg tgcgcgcgct cgcgctgctg ggcgacagcg tcacgaccga
     3781 ccacatctcg ccggcgggca acatccggcg cggcacaccg gccgccgcgt ggctcgaaga
     3841 gcacggcgtc gaaccgcgcg acttcaactc ctacggtgcg cgccgcggca accacgaggt
     3901 gatgatgcgc ggcacgttcg cgaacgtgcg cctccggaac cgactcgcac ccgggaccga
     3961 gggcggcgtt acgttgcatc tgcccgatcg cgaagagacg accatctacg acgccgcgat
     4021 gcactacgcg cgcgacggcg tgccgctgct cgtgctcgcg gggaaggaat acggatcggg
     4081 cagctcgcgc gactgggcgg ccaagggttc gttgttgctc ggcttgcgcg ccgtcatcgc
     4141 agtgtcgttc gaacggatcc accgttcgaa tctcgtcggc atgggtgtcc tgccgctcga
     4201 gctcgccgac ggcgcaaccg tcgagagctt gggcttgacc gggcacgaga cgttccggat
     4261 caccggtctc gacgcgctgg acggcgacgg accactgcct ttttccgtca ccgtggcggc
     4321 cgacgatcgc acgttcagga cgaccgcacg ggtggacaca ccgttcgaac gagaggtttt
     4381 tctcgcgggc ggcattcttc cgttcaccct tcgtcgtctt ctgacctgac tgtttccccg
     4441 gcgccgaaaa tcgggtaatc gtggcgcatg ggtgaaacgg caatgaggct cgatgtcgag
     4501 atcgaagaca acgatgacga gcgcgtcgcc aggctcgaag gaatggtcga cgtcttcacc
     4561 gcgggtggtg tcgtgcgccg agtgttggcg ggtctcccgc gcgaggcgac gcgggtcgtg
     4621 atcgacctgc gcggcgtgcg cttcatggac tccgcgggac tcagtgcagt cgtccgcctg
     4681 tcggaacgag cccgccgccg cgcgctcgac ttgcgcgcgg agctcggtga cgcgtcgcga
     4741 ctgaacccga cggtggccgg gatgttgcgc cgcgtcgtgc cctgcgacga ggacaagcac
     4801 gcgagacccg ccgccagcta gcgctcaagc gagcaggtcg aaggcgacaa agaggcatgg
     4861 gggcgcggcg atgtggctcg atgagctcgc cttcgagctc gtcgggcgcc acatcgccgg
     4921 ccctcccgtt gccccccgct cacccgccgc ccggaggccc cgcccggatc cccgaaccgc
     4981 ctgtgccgcc actggcatgt cgaacgcccc aaggggacga tgttcgtgtg ggcgccgatc
     5041 cccgagccct acgccgagat gtcgagcatc gagttcgcga cgatgtgcgt gcaggaag
//
LOCUS       DASUGA010000877         2342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1362512, whole genome shotgun sequence.
ACCESSION   DASUGA010000877 DASUGA010000000
VERSION     DASUGA010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362512"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VFX21_17305"
     CDS             <1..471
                     /locus_tag="VFX21_17305"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX5097783.1"
                     /translation="GLETVTIRYFNVFGPRQDPSSPYSGVISVFAKALLENTSPTIYG
                     DGEQTRDFTYVANVVDGVLRAATAPKASGQVINVATESRISLNRLFSTMRDLVGSGVN
                     VSYGPTRSGDVRDSQADITRAREILGYKPIVSFEEGLRQTLEWYRTQGDAVARR"
     gene            813..1127
                     /locus_tag="VFX21_17310"
     CDS             813..1127
                     /locus_tag="VFX21_17310"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEX5097784.1"
                     /translation="MNRSALALVAALVCFSPWSADAQRASKPPVAAPTEVVNLNTGTA
                     TQIASLPGIGLKTAELVVQYREKNGPFKKIEEIMNVRGVGEKSFLRIKDRLTVAQPKS
                     EK"
     gene            1130..1711
                     /locus_tag="VFX21_17315"
     CDS             1130..1711
                     /locus_tag="VFX21_17315"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEX5097785.1"
                     /translation="MRHKGYSVLELLMVVSVSIVISGAAIPLATNGVDRTRAEGAVRY
                     VAGRMASARFEAVRRSAYVAIRFVPQSDGIWLRAFVDGNRNGVLSGDITRGLDVPITS
                     DERLDHHFSGITFGILPNVTGLDPGQPFNAGDPVQIGSSTLLSFSPLGASTSGTLFIR
                     GQRGTQFAVRILGATGRTRTFEFNFGDARWHRR"
     gene            1696..2025
                     /locus_tag="VFX21_17320"
     CDS             1696..2025
                     /locus_tag="VFX21_17320"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX5097786.1"
                     /translation="MAQTVAAERRAAPRVSPDRAGGMDRARLRPGRSARIINLSSVGA
                     LIETDWRLMPGLRVEVQIGDPAPLCKVSARILRCHVALLAREQVRYRAGLSFEERIPF
                     GEETTPT"
     gene            2108..>2342
                     /locus_tag="VFX21_17325"
     CDS             2108..>2342
                     /locus_tag="VFX21_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5097787.1"
                     /translation="MVTPQSPNNSMAYTLFQRHVMRRDGAAALVGSSAVMRSVRERIE
                     RVAATDFTVLIDGESGVGKELVARQIHDISGRRR"
BASE COUNT          420 a          773 c          753 g          396 t
ORIGIN      
        1 ggcctcgaga cggtcaccat ccggtacttc aacgtcttcg gaccgcggca ggatccgtct
       61 tcgccctact cgggcgtcat ctcggtcttc gccaaggcgc tgctcgagaa cacgtcgccg
      121 accatctacg gcgacggcga gcagacgcgc gacttcactt atgtcgccaa cgtcgtcgac
      181 ggcgtgctgc gcgcggcgac cgcgccgaag gcgtcgggcc aggtaatcaa cgtcgcgacc
      241 gagtcgcgga tctcgctcaa tcggctcttc agcacgatgc gcgatctggt tggaagcggc
      301 gtcaacgtca gttacgggcc gacgcgatcg ggcgacgtgc gcgattcgca ggccgacatc
      361 acccgcgccc gcgagattct cggctacaaa ccgattgtgt cgttcgaaga aggcctgcgg
      421 cagacgctcg agtggtaccg cacgcaaggc gacgcggtcg ccaggcgata gcaacccccg
      481 tagacagctt tttcctcctc ggacattcct tggccgcgcg cgaatagaag cgcgcgcgcc
      541 aaacggcacg cgctcaatca catcccacga cgattcacga tcgcgccgcg cggcgcgcgg
      601 cttgccatag gtctttccgg ccctggtcat ctgcgcgcgt gtgacggtgc ctgaccccat
      661 cgaaatatgc cgggtaacca ccagggccat cttttgccca ctgtcgtgcg cgccacagtc
      721 catgtcgtgc gcatcaacgc cgagcgacca acggtcgcga ggtacaggcg gcggggcggg
      781 agccccgcca cacaccaagt aaggaggaca gaatgaatcg ttcagcgctt gccctcgtcg
      841 ccgccctcgt ctgtttcagc ccgtggtccg cggacgccca gcgcgcctcg aagccgccgg
      901 tcgccgcgcc gacggaggtc gtcaacctca acaccggcac ggcgacgcag atcgcctcgt
      961 tgccgggcat cggcctcaaa accgccgagc tcgtcgtgca gtaccgcgag aagaacgggc
     1021 ccttcaagaa gatcgaagag atcatgaacg tgcggggagt cggcgagaag agcttcctcc
     1081 gcatcaagga tcgcctcacc gtcgcgcagc caaagagcga gaagtagtcg tgcggcacaa
     1141 gggctactcc gtactcgagc tgctcatggt ggtcagcgtc tcgatcgtga tcagcggtgc
     1201 ggccattccg ctcgccacga acggcgtcga ccggacgaga gccgaaggag cagtccgcta
     1261 cgtggccggg cgcatggcaa gcgcacgttt cgaggccgtc aggcgctcgg cctacgtcgc
     1321 catccggttt gtgccgcaga gcgatggcat ctggcttcgc gcgttcgtcg atggcaaccg
     1381 caacggcgtg ttgtccggcg atatcacgcg tggcctcgac gtgccgatta catccgacga
     1441 gcggctcgac catcatttct caggcatcac gttcggtatt ctgcccaacg tcacggggct
     1501 cgacccgggg cagccattta atgcgggcga tcccgtgcaa atcgggtctt cgaccctgct
     1561 cagcttcagt ccactggggg catcgacctc gggcacgctc ttcattcgcg gccaacgggg
     1621 cacccagttc gccgtgcgca tcctcggcgc caccggccgc acccgcacgt tcgagttcaa
     1681 tttcggagat gcccgatggc acagacggta gccgccgaac gccgcgcggc accgcgagtg
     1741 tcgccagaca gggcgggcgg aatggatcgg gcgcgcctgc gaccggggcg gtcggcgcgc
     1801 atcatcaatc tgtcgtcggt cggtgcgctc atcgaaaccg actggcgttt gatgcccggg
     1861 ttgcgcgtcg aggtgcagat tggcgatccc gcgccgctgt gcaaggtaag cgctcgtatt
     1921 ctccggtgcc acgtggctct cctggcccgc gagcaggtga ggtatcgggc gggcttgtca
     1981 ttcgaagaac gcattccctt tggggaagaa acgaccccga cctagggccg ggtatcacgg
     2041 agcgtacgag attgcgggtc gcagcaactg ggggaagaca ctaccccgga ggttcaggaa
     2101 cttggacatg gtcactcctc aatccccgaa caattcgatg gcatacacct tgtttcagcg
     2161 tcacgtcatg cgacgagacg gcgcggcagc gcttgtcggc tcgagcgcgg tgatgcgcag
     2221 cgtgcgcgag cgcatcgagc gggtggccgc caccgacttc accgtcctca tcgatggcga
     2281 gagtggtgtc ggcaaagagc tcgtcgcgcg acagattcac gacatcagcg ggcggcggcg
     2341 cg
//
LOCUS       DASUGA010000878         2478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_252649, whole genome shotgun sequence.
ACCESSION   DASUGA010000878 DASUGA010000000
VERSION     DASUGA010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252649"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VFX21_17330"
     CDS             <1..460
                     /locus_tag="VFX21_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5097788.1"
                     /translation="DGTRVFYGLAGDRVGELWRALRAVATEHVGGIDRLAQAYLGDRD
                     GVATISRHDLAARLRQRSVVVLDVRPEAEFAAGHIAGARSVPPGELRQRLRDVPKSRG
                     IVAYCRGPFCVFADDVVRELRGRGIRACRLEDGFPEWRDAGLPIAVGQGG"
     gene            463..1068
                     /locus_tag="VFX21_17335"
     CDS             463..1068
                     /locus_tag="VFX21_17335"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5097789.1"
                     /translation="MTEDLLVDVDTLREQVREKYREVAVEPGAKYHFHTGRPLARKLG
                     YDTAIVDALPDRAVESFAGVGNPFSMRTIGSGERVVDVGSGAGFDSFVAASLVGTEGR
                     VIGVDMTAAMLDKSRTTAAQFGFAHVEFREGFAEALPVDDEWADAVISNGVINLCADK
                     RAVFGEISRVLRPGGVLQFADIANGRPVPPEALRDIDLWTG"
     gene            1105..1251
                     /locus_tag="VFX21_17340"
     CDS             1105..1251
                     /locus_tag="VFX21_17340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097790.1"
                     /translation="MLEDTGFTDVQIGPPVDTFGGATGEEKARAYEVYGYAFIARKRH
                     ERAV"
     gene            1304..1663
                     /locus_tag="VFX21_17345"
     CDS             1304..1663
                     /locus_tag="VFX21_17345"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5097791.1"
                     /translation="MRARVDLVLDCTEPAKLVQFWREALDYREYFTDTSVAVLVPKEG
                     NAPPLVLQGVPEPRVTKNRMHLDIVVDDIEREVKRLEALGAHRLDDGHQHFGDTKWIR
                     MSDPEHNEFCVCTGVEW"
     gene            complement(1673..2158)
                     /locus_tag="VFX21_17350"
     CDS             complement(1673..2158)
                     /locus_tag="VFX21_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5097792.1"
                     /translation="MTTQADETTVRTEVVVNAPVERAFALFTERFDQIKPREHNMLGV
                     DIAASVFEARVGGRVYDRGTDGTECQWGRVLAYEPPTRLVFSWDISPHWQIETDLGRA
                     SEVEVRFVADGPTRTRVELEHRNLDRHGDGWEGTREGVRGDQGWPLYLQRFAAVVTAD
                     R"
     gene            complement(2155..2457)
                     /locus_tag="VFX21_17355"
     CDS             complement(2155..2457)
                     /locus_tag="VFX21_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5097793.1"
                     /translation="MDAWTALADPTRRTILELVAVRPRAVGELAELLPVTRPAVSQHL
                     KSLKTAGLVLDRAEGTRRIYRANPQGLAELRAQLDRFWTEALANFAKVVDEAEEEA"
BASE COUNT          410 a          804 c          836 g          428 t
ORIGIN      
        1 cgacggaacg cgggtgttct acgggttggc gggcgatcgc gtcggcgagc tttggcgggc
       61 gttgcgcgcc gtggcgaccg agcacgtcgg cggaatcgac cgactcgccc aggcgtacct
      121 cggcgatcgc gacggtgtgg cgacgatcag tcgccacgac ctggccgcgc gcctgaggca
      181 gcgaagtgtc gtggtcctcg acgtccgccc cgaagccgag ttcgcggcgg gccacattgc
      241 cggcgcgcgg tcggtgccac ccggcgaact tcggcagcgg cttcgtgacg tgccgaagag
      301 ccgcgggatc gttgcgtact gccgggggcc gttctgcgtg tttgcagacg acgtggtccg
      361 tgagctccga ggccgcggca tacgcgcgtg ccggctggag gacggtttcc cggaatggcg
      421 agacgcggga ctgccgatcg cagtcggcca gggaggctga gcatgaccga agatctgttg
      481 gtcgacgtcg acacactgcg cgagcaggta cgcgagaagt accgcgaggt tgcggtcgaa
      541 cccggcgcca agtaccactt ccacaccggc cggccattgg cgcgcaaact cggctacgac
      601 accgcgatcg tcgatgcgct tcccgaccgc gcggttgagt ccttcgccgg cgtggggaat
      661 cccttctcga tgcggacaat cggctcgggt gaacgcgtcg tcgacgttgg atcaggagcg
      721 ggatttgatt cgttcgtcgc ggcgtcgctc gtcggtaccg aaggtcgtgt gatcggcgtc
      781 gacatgaccg cggcgatgct cgacaagtca cgcacgaccg ccgcacagtt cgggttcgcg
      841 cacgtcgagt tccgtgaggg tttcgccgag gcgcttcctg ttgatgacga gtgggccgac
      901 gcggtgatct cgaatggcgt catcaacttg tgtgcagaca agcgcgcggt cttcggcgaa
      961 atcagtcgcg tgctccggcc cggtggcgtg ttgcagttcg ccgacatcgc caatggccgg
     1021 ccggtcccac ccgaggcact gcgcgacatc gacctctgga ccggctgaat tgccggcggg
     1081 ctgccccgtg cgggttggca gacgatgctc gaggacaccg gcttcaccga cgtacagatc
     1141 gggccgccgg tcgacacgtt cggtggcgcc acgggcgagg aaaaggcccg cgcctatgag
     1201 gtctacgggt acgcgttcat cgcgcgcaag cggcacgagc gcgcggtgtg actcgcgcac
     1261 gttcctgtgc tccatcagca caaccgaccg atggagggca tccatgagag caagggttga
     1321 tctggtactc gactgcactg agccggcgaa gctcgtccag ttctggcgcg aagcgctcga
     1381 ttaccgcgag tacttcaccg acacgtccgt cgcggtcctc gtgccaaagg agggcaacgc
     1441 tcccccgctc gtgctgcaag gcgtacccga gccgcgcgtc accaagaacc gcatgcatct
     1501 cgacatcgtc gtcgacgaca tcgagcgcga agtcaaacga ctcgaagcac ttggtgccca
     1561 tcgcctcgac gacgggcacc agcacttcgg cgacaccaaa tggatacgaa tgtcggaccc
     1621 ggaacacaat gagttctgcg tgtgcacggg tgtcgaatgg tgactcgcga gctcaccgat
     1681 cggctgtgac aaccgctgcg aaccgctgca ggtagagcgg ccagccctgg tcgccacgaa
     1741 ctccttcgcg cgtgccttcc caaccgtccc cgtgacggtc gaggttgcgg tgttcgagtt
     1801 cgacgcgcgt gcgcgtcgga ccgtcggcga cgaaccgcac ttctacctcg ctggcccggc
     1861 cgagatcggt ctcgatctgc caatgcgggc tgatgtccca actgaacacg aggcgggtcg
     1921 gcggctcgta cgcgagtacg cggccccact ggcattcggt tccgtcggtg ccccggtcgt
     1981 agacgcgtcc acccacgcgc gcctcgaaca cggatgctgc gatgtcgaca ccgagcatgt
     2041 tgtgctcgcg cggcttgatc tgatcgaacc gctcggtgaa gagcgcaaag gcgcgctcga
     2101 cgggcgcgtt gacgacgact tcagtacgca ccgtcgtttc gtcggcctgt gttgtcatgc
     2161 ttcctcctcg gcttcgtcga caacctttgc gaagttggcg agcgcttccg tccagaaccg
     2221 atcgagctgc gcacgcaact cggcgagacc ctgcgggttg gctcggtaga tccgtctcgt
     2281 cccctcggcg cgatcgagaa cgaggccggc cgtcttcaac gacttcagat gttgagagac
     2341 ggcggggcgc gtcaccggca gcagctcggc gagctcaccg accgcccgcg ggcggaccgc
     2401 gaccaactcg aggatggttc gcctcgtcgg gtcggcgagc gccgtccacg cgtccatatc
     2461 ggtaagctat cactaacg
//
LOCUS       DASUGA010000879         4183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_807080, whole genome shotgun sequence.
ACCESSION   DASUGA010000879 DASUGA010000000
VERSION     DASUGA010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807080"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..576)
                     /locus_tag="VFX21_17360"
     CDS             complement(13..576)
                     /locus_tag="VFX21_17360"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5097794.1"
                     /translation="MVDTDLDPRVERSRRVILEAALEELGRVGYGALTIEGVAARAGV
                     GKSTIYRHWPGKLALVEDAFRTLKAEVTVPEHGTLRERLTTYLTEVATMAVQSTYSAC
                     MPALIEAAARDEQVREFHCKFTQERMGGLADTLRKAIGSGELPPSTNPELLADALMGP
                     IVMRRLMTSEPFPPDQVPALVNQLIPA"
     gene            complement(581..2212)
                     /locus_tag="VFX21_17365"
     CDS             complement(581..2212)
                     /locus_tag="VFX21_17365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5097795.1"
                     /translation="MASVSPPDLARPIPLHEQPQIHSRRWFLLAMMCLSLVLVVMSVS
                     GLVTAIPTMQEELHASASQVQWILDAYAVVFAGTLLTAGALGDRFGRKRALLTGLVVF
                     GAGAFFAGIASSASQVIAGRAVMGIGAALVMPATLSIITTIFPPEERSRAIAVWAGFA
                     GAGGALGPIVSGALLENFWWGSAVLVNLPIVAAVFLAIWAFAPESRDENETPLDPVGA
                     VLSLVGLSALVFGIIQGGEDGWTSTPVVGAFAVAIAAIAGFLSWERRSSHPMLPLEFF
                     KNRRFSMGAGVITIAFFVMFGFFFLTTQYLQFARGFSPLEAGLALLPLPIMFVAFSPR
                     SAVLAERYGAPRVMASGLVIVAGGFVMLSLLSPGTPYLLIALAYAVLGIGMSVTAAPA
                     TGEIMSAVPLSKAGVGSAVNDTTRELGGALGIAILGSIANSAYRSGIDLSGIGLAGTP
                     RGNAEDSIGAAVGVASQAPGGAGTEVKVRAAQAFTDAFNLAMAVSVGLALVAAAAVIL
                     FARPRRERTDEVPVEDLAEELDWALVPVGAGERSE"
     gene            2457..3824
                     /locus_tag="VFX21_17370"
     CDS             2457..3824
                     /locus_tag="VFX21_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5097796.1"
                     /translation="MAVFRQLLVNTLISGVTSSFLWFALTFWVYIETHSVVATGVIGA
                     AYSLCTAALGPVFGTFVDRHRKHTVMTLATLTSTICFTITTAIYVALRADDLLQLRSP
                     WFWVLVAFTLFGSVASMMRNIALSTTVTLLVPDDRRDRANGMVGTVTGVSFAITSVFS
                     GLIIGMAGMGWAFAVALVLTVVALVHLGTITIDEPKPAPAPGEPDGERKKYVDFRGAL
                     DAIHAVPGLMMIILLAAFNNLLMGVFMALMDAYGLSLVSVETWGLLWGLISLSFIAGG
                     FYVARRGLGRNPLRVIVLGNLVNWIICTFFAVRSSIALLVVGMALWLALVPVIEAAEQ
                     TVLQRAIPFERQGRVFGFAQLVENAAAPLTAIMMAPIAEALFMPFMTDGRGADWIGGI
                     YGTGPERGLALMFTLAGLIGIVVTAVAWTSRSYRRLTVAGGYLQDGDEPRAPYAPPPS
                     AANEQ"
     gene            complement(3739..4170)
                     /locus_tag="VFX21_17375"
     CDS             complement(3739..4170)
                     /locus_tag="VFX21_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5097797.1"
                     /translation="MVATVAEAVALLTPLTDEEARARPDPQTWSPLEYACHLRDVFLV
                     QRERVLLARRVEEPDLVPMGRDERAVHDGYAEQDPEIVVRQLTDAALLFANVLARLDD
                     DAWERTVIYNYPAPTARSLRWVAVHTEHEARHHLGDIRRQL"
BASE COUNT          675 a         1439 c         1400 g          669 t
ORIGIN      
        1 ccccccgacc gactacgccg ggatcaactg gttgacgagc gccggcactt ggtcgggcgg
       61 gaacggttcg ctcgtcatca ggcggcgcat cacgatcggg cccatcagcg cgtcggccag
      121 caactcgggg ttcgtcgacg gcggcagctc gccgctgccg attgccttcc gcaacgtgtc
      181 cgcgagcccg cccatgcgtt cctgcgtgaa cttgcagtgg aactcgcgca cttgctcgtc
      241 gcgcgcggcg gcctcgatga gggcgggcat gcatgcggag taggtcgact gcacggccat
      301 cgtcgcgact tcggtgaggt acgtcgtcag gcgctcgcgc agcgtgccgt gctcgggcac
      361 ggtcacttcc gctttcaagg tgcggaacgc gtcctcgacg agcgcgagct tgcccggcca
      421 gtgccggtag atcgtgctct tgccgacgcc cgcacgcgcc gcgacgccct cgatggtgag
      481 ggcgccgtaa ccgacccggc ccagctcttc gagcgcggct tcgaggatca ctcgccgcga
      541 acgctcgacg cgcgggtcca ggtcggtgtc caccacggcc tcattctgac cgttcgccgg
      601 cgccgacggg aaccagggcc cagtcgagct cttccgcgag gtcctcgacc ggcacctcgt
      661 cggtgcgctc ccggcgcggc cgggcgaaca ggatcaccgc cgcggccgcg accagcgcga
      721 gcccgaccga gaccgccatc gcgaggttga aggcgtcggt gaacgcctgc gcggctcgca
      781 ccttcacctc ggtgcccgcg ccgcccggcg cttggctcgc gacaccgacg gccgcaccga
      841 tcgagtcctc ggcgttccca cgcggcgtgc ccgcgaggcc gatacccgac aaatcgatgc
      901 ccgagcggta cgcgctgttg gcgatgctgc cgaggatcgc gatccccagc gcgccaccga
      961 gctcacgcgt cgtgtcattg accgcggacc cgacaccggc cttcgacaac ggcaccgcgc
     1021 tcatgatctc gccggtcgcg ggtgccgcgg tgacgctcat accgatgccg agcaccgcgt
     1081 acgcgagcgc aatgagcagg tagggcgtgc ccgggctgag cagtgacagc atcacgaagc
     1141 cgcctgcgac gatcacgagg cccgatgcca tcaccctcgg tgcgccgtag cgttccgcca
     1201 gcaccgcgct acggggtgag aacgcgacga acatgatcgg caacggcaac agtgcgagcc
     1261 ctgcttcgag cggcgagaac ccacgagcga actgcaggta ctgcgtcgtc aggaagaaga
     1321 agccgaacat cacgaagaac gcgatcgtga tgacgccggc gcccatgctg aagcggcggt
     1381 tcttgaagaa ctcgagcggc agcatcgggt gcgagctgcg ccgctcccat gacaggaagc
     1441 cggcgattgc ggcgatcgca accgcgaacg caccgacgac gggcgtcgac gtccagccgt
     1501 cttcgccacc ttggatgatg ccgaacacga gcgcgctcag gccgacgagc gacaggaccg
     1561 cgccaacggg gtcgagcggc gtttcgttct cgtcccgcga ctcgggtgcg aacgcccaga
     1621 tggcaaggaa caccgccgcg acgatcggca ggttgacgag caccgcggat ccccaccaga
     1681 agttctcgag cagcgcgccg gagacgatgg gcccgagtgc accgcccgcg cccgcgaacc
     1741 cggcccacac cgcaatcgcg cgggaccgtt cctcgggcgg gaagatcgtc gtgatgatcg
     1801 acaacgtcgc gggcatgacg agtgcggcac cgatgcccat gaccgcgcgt ccggcgatca
     1861 cctgtgacgc gctcgacgcg atgccggcga agaacgcgcc tgcaccgaag acgacgaggc
     1921 cggtgagcag cgcgcgcttg cggccgaacc ggtcgccgag ggcgcccgcg gtgagcagcg
     1981 tgcccgcgaa cacgacggcg tacgcgtcga ggatccactg gacctgcgac gccgatgcgt
     2041 gcagctcttc ttgcatggtc gggatggcgg tgacgagtcc cgagaccgac atcacgacga
     2101 ggaccagcga gaggcacatc atcgccagca ggaaccagcg gcggctgtgg atctgaggct
     2161 gctcgtggag cggaatcggg cgcgcgaggt caggggggct gactgaggcc atggtgcggt
     2221 ctcctccggc gggggtccga ggaccgaggc ttgcgatacg ttctcgtttc acagtgaaac
     2281 gtacacgtct cactatgaaa cgtcaacgtc ttatttctcg gaacgcggcg ggtcggcctc
     2341 ccgtacgcga cgatgagtcg cccgaccgag cgcagcctca gtcccgtgac cgccgtggcc
     2401 gatccaactt ggctgacaac cgcacccaga gcccgccccc gccggacgag cggtcgatgg
     2461 ccgtcttccg tcagctactc gtcaacacgc tcatcagcgg cgtcaccagc tcgttcctgt
     2521 ggttcgcgct gacgttctgg gtctacatcg agacgcactc ggtcgtcgcg accggcgtga
     2581 tcggagcggc gtacagcctc tgcaccgccg cgctcgggcc cgtcttcggc acattcgtcg
     2641 accggcaccg caagcacacg gtcatgacgc tcgcgacact gacgtcgacg atctgcttca
     2701 cgatcacgac cgcgatctac gtcgcgctgc gcgcggacga tctgttgcag ctgcgcagcc
     2761 cctggttctg ggtactcgtc gcgttcacat tgttcggatc ggtcgcgtcg atgatgcgca
     2821 acatcgcgct gtcgacgacg gtcacgctcc tcgtcccgga cgatcgccgc gaccgcgcga
     2881 acggcatggt cggcaccgtg accggcgtgt cgttcgcgat cacgtcggtg ttcagcggtc
     2941 tcatcatcgg gatggccggc atgggatggg cgttcgccgt cgcgctcgtg ctcacggtgg
     3001 tcgcactcgt ccacctcggc acgatcacga tcgacgaacc gaaaccggca ccggcacccg
     3061 gcgaacccga cggtgagcga aagaagtacg tcgacttccg gggcgcgctc gacgcgatcc
     3121 acgcggtgcc ggggctgatg atgatcatcc tcctggccgc gttcaacaat ctgctcatgg
     3181 gcgtgttcat ggcgctcatg gacgcgtacg gcctgtcact cgtctcggtc gagacgtggg
     3241 gattgctctg ggggttgatc agcctctcgt tcatcgcggg tggcttctac gtcgcgcgac
     3301 gcggtctggg ccgaaaccca ctgcgcgtca tcgttctcgg caacctcgtc aactggatca
     3361 tctgcacgtt cttcgcggtg cggtcgtcga tcgcgctgct cgtcgtcggc atggccttgt
     3421 ggctcgcact cgtgccggtc atcgaggcgg ccgaacagac cgtgctgcaa cgggccatcc
     3481 cgttcgagcg tcagggtcgc gtcttcggat tcgcgcaact ggtcgagaac gcggccgcgc
     3541 cgctcaccgc gatcatgatg gcgccgatcg cggaagcgtt gttcatgccg ttcatgaccg
     3601 acgggcgggg cgcggactgg atcggcggga tctacggcac cggccccgaa cgcgggctgg
     3661 cgttgatgtt cacgcttgcc ggcctcatcg gcatcgtcgt gaccgccgtt gcgtggacgt
     3721 cgcgctcgta tcgccggctc acagttgccg gcggatatct ccaagatggt gacgagcctc
     3781 gtgctccgta tgcaccgcca cccagcgcag cgaacgagca gtaggcgccg gatagttgta
     3841 gatgaccgtt cgttcccacg catcgtcgtc gaggcgcgcg agcacgttgg cgaacaacag
     3901 cgcggcgtcg gtgagctggc gtacgacgat ctcgggatct tgttccgcgt agccgtcgtg
     3961 caccgcgcgt tcgtcgcgcc ccatcggcac gaggtcgggt tcttccacgc ggcgcgccag
     4021 cagcaccctc tcgcgctgca cgaggaacac gtcgcgcaga tgacacgcgt actcgagcgg
     4081 tgaccaggtc tgcgggtcgg gccgggcgcg cgcctcttcg tccgtcaacg gcgtgagcag
     4141 cgcgacggct tccgcgaccg ttgcgacgat cgcaacgtcg gcg
//
LOCUS       DASUGA010000880         5435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_807492, whole genome shotgun sequence.
ACCESSION   DASUGA010000880 DASUGA010000000
VERSION     DASUGA010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807492"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="VFX21_17380"
     CDS             complement(<1..1094)
                     /locus_tag="VFX21_17380"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5097798.1"
                     /translation="MALGAAAVALLTSGCGWLQLGANAGRTSFNKDETKLTAATLGSV
                     TQLWSQTEQGTATAPIVNDNTLYVATGATMTAYNATTGAVVWQKSVAKVGGTVVLQAP
                     FWYKGRVGAFYSEKEDTHASQLNGIVDFEYDATTGEVHANDVSFGAMIVPRGFVARGT
                     EFAATATARASGQSAAAVSYGADSTATDNKTGILSLTATDWPTQPMMLGRRVFVSAGN
                     KVFAFDMDSCPASTVLPATGFCGWTWSVTLEPDDPWALAGMPVAIGDDAIAVPVDGGE
                     LRVLDAATGAEQWRGSVGTNDRGLAVADGVIYSVSPDDRLYAWNASGCGAATCDPLWA
                     GIVEFTPMTAPVVAGGLVYVGSANHVRAFPV"
     gene            1350..2444
                     /gene="nadA"
                     /locus_tag="VFX21_17385"
     CDS             1350..2444
                     /gene="nadA"
                     /locus_tag="VFX21_17385"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX5097799.1"
                     /translation="MLRLQAPLPDDYTLASDAELVDRIAAAKAALGDRLLILGHHYQR
                     DEVIRWADQIGDSFKLARFAADNHRATDIVFCGVHFMAESADVLTADHQRVVLPDLNA
                     GCSMADMANIDQVEEAWDEIAAVTAIDRVIPITYMNSSAALKAFVGRHGGAVCTSSNA
                     RAVLEWAFARGDKVLFFPDQHLGRNTGFQMGYTEGDMRVWDPRHDLGGLEEREVKDAT
                     FLLWKGHCSVHQRFRPEHVAAARAEHAGVEVVVHPECAHDVVELADRVGSTERIQAWV
                     QDAPAGTVMAIGTEIHMVQRLAAQHPDKTIVSLDPLICPCSTMFRIDAPHLAWVLERL
                     VAGEVVNQIVVDDDTSEWAHVALQRMLDIT"
     gene            complement(2437..3168)
                     /locus_tag="VFX21_17390"
     CDS             complement(2437..3168)
                     /locus_tag="VFX21_17390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5097800.1"
                     /translation="MSDIDRADWERNAQWWQDGFTDGADPEYEEQIIPLARECLAGAS
                     RVVDVGTGEGQLARVAVDLGADVVVGLDPTSAQLRVARERGGGPAYAQGLAEAMPFAD
                     AGFDTALACLVFEHIPDHEPAIAEVARVLAPGGQFVFFLNHPLLQAPNSGWVVDHILD
                     EQYWRIGPYLEPDVTMEELAPGVVLPFVHRPLSQYVNAMAKQGLLIERMEEPMPPEGF
                     LARAEEYRAAATIPRLLLFVARKLR"
     gene            complement(3165..>5435)
                     /gene="uvrA"
                     /locus_tag="VFX21_17395"
     CDS             complement(3165..>5435)
                     /gene="uvrA"
                     /locus_tag="VFX21_17395"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX5097801.1"
                     /translation="DRLVRRANIERRLTDSLETALKIAEGVAEVEIVPREGEEAPKAQ
                     PSGAVGGVGGWETLTFSEHLACTHCGLSYDELAPRNFSFNSPYGACETCDGLGTKFQV
                     DGELVIADDDLSIAEGAIAPWSGYRGEYFSRVLQAVGADNGFSVDTPWKKLKAAQKKI
                     VLYGTGGKTVTVRYKNRYGRQRQYTAKYEGVIPWVERRHAESDSDRVREQIEGYMRLV
                     PCPACGGARLKPASLAVTVGGKNLFEVGEMSIGDAADFFRTVELSERDRMIAERVFKE
                     VVERIQFMLDVGLDYLSLNRSSGTLAGGEAQRIRLASQIGSGLVGVLYVLDEPSIGLH
                     QRDNKRLIETLMRLRNLGNTVIVVEHDEETIRVADHIVDIGPGAGEHGGAIVAEGSLK
                     DILKTKASITGQYLSGKRSIPVPEMRREPGDAWIAVRGAREHNLQNIDVEIPLGCFVA
                     VTGVSGSGKSTLVGDILHPALMQKIYRSREVPGRHKKIEGANQLDKVINIDQSPIGRT
                     PRSNPATYTGVFDKIRNLFATTPEAKVRGYQQGRFSFNVKGGRCEACQGDGTIKIEMH
                     FLPDVYVPCEVCKGARYNRDTLDITFKGKNIAEVLDMPAEEALEFFTNQPTIARQLQT
                     LVDVGLGYVRLGQPAPTLSGGEAQRVKLSTELGKRSTGRTIYILDEPTTGLHFEDISK
                     LLGVLQGLVDKGNTVIVIEHNLDVIKSADWVIDLGPEGGTGGGTVVAEGTPEQIAKVR
                     ESHTGRFLAPLLGIDS"
BASE COUNT          836 a         1947 c         1763 g          889 t
ORIGIN      
        1 accgggaacg cgcgaacgtg gttcgcgctc ccgacgtaca cgaggccgcc cgcgacgacg
       61 ggcgcagtca tgggcgtgaa ctcgacgatg ccggcccaca gcggatcgca cgtcgcggcg
      121 ccacagccac tcgcgttcca cgcgtagagc cggtcgtccg gcgacaccga gtagatgacg
      181 ccatcggcga ccgcgaggcc gcgatcgttc gtcccgaccg aaccccgcca ctgctcggcg
      241 ccggtcgcgg catcgagcac gcgcagctcg ccgccgtcca cgggtactgc aatcgcgtcg
      301 tcgccgatcg cgacgggcat gccggcgagc gcccacgggt cgtcgggctc gagcgtgacg
      361 ctccacgtcc agccgcagaa cccagttgcc gggagcacag tcgacgccgg gcacgaatcc
      421 atgtcgaacg cgaacacctt gttgcccgcc gagacgaaga cgcgccgccc aagcatcatc
      481 ggttgcgtcg gccagtcggt tgccgtgagc gacaggatcc cggtcttgtt gtcggtcgcg
      541 gtgctgtccg cgccgtacga gacggcggcg gcggattggc ccgacgcgcg ggcggtcgcg
      601 gtcgccgcga actcggtgcc gcgcgcgacg aagccgcgcg gcacgatcat ggcgccgaag
      661 gagacgtcgt tggcatggac ctcgccggtc gtcgcgtcgt actcgaagtc gacgatgccg
      721 ttcagctgcg acgcgtgcgt gtcctccttc tcgctgtaga aggcgccaac gcggcccttg
      781 taccagaacg gtgcttggag cacgacggtc ccgccgacct tcgcgaccga cttctgccag
      841 acgaccgcac cggtcgtcgc gttgtacgcg gtcatcgtcg ctcccgtggc gacgtacaac
      901 gtgttgtcgt tgacgatcgg cgcggtcgcc gtgccttgtt cagtctgcga ccacaactgc
      961 gtcaccgagc cgagcgtcgc cgcggtgagc ttggtctcgt ccttgttgaa cgacgtccgt
     1021 cccgcgttgg cgccgagctg cagccaaccg cagccgctcg tcagcaacgc cactgcggcc
     1081 gcacccagcg ccaacgtcca tgtccctcgc gcgcgcatcc ctgccccccg atcaccgcga
     1141 atcggataaa agtagcagaa aggaccggaa tggccgcgag gaatcttcct ctcgacgtga
     1201 gtgttatgct cccggagacc cgtcgggccc ggtatcccgg cggtgacata tcaggctccg
     1261 aggtctacaa ccccgcagtg ctggcgaccg ggctgtcacc tgcagaccgc tgaggagcgt
     1321 ggccccagaa aacggggagg cccccgatca tgttgcgact gcaggctcca ctgccggacg
     1381 actacaccct cgcgtcggat gccgaactgg tcgatcgcat cgccgcggcc aaggccgcgc
     1441 tcggtgaccg gctcctgatc ctcgggcacc actaccagcg cgacgaagtg atccgctggg
     1501 ccgaccagat cggcgactcc ttcaagctcg cccgcttcgc cgcggacaac caccgcgcca
     1561 ccgacatcgt gttctgcggc gtgcacttca tggccgagtc cgccgacgtg ctcaccgccg
     1621 accaccagcg cgtcgtcctt cccgacctca acgcgggctg ctccatggcc gacatggcga
     1681 acatcgacca ggtcgaagag gcgtgggacg agatcgccgc cgtcaccgcg atcgaccgcg
     1741 tgatcccgat cacgtacatg aactcgtcgg ccgcgttgaa ggcgttcgtc ggccgccacg
     1801 gcggcgccgt gtgcacgtcg tcgaatgcgc gcgccgtgct cgaatgggcg ttcgcacgtg
     1861 gcgacaaggt gctcttcttc cccgaccagc acctcggccg caacaccggt ttccagatgg
     1921 gctacaccga gggcgacatg cgcgtctggg acccgcgcca cgacctcggc ggcctcgagg
     1981 agcgtgaggt caaggacgcg acgttcctgt tgtggaaggg ccactgctcg gtgcaccagc
     2041 gcttccggcc cgagcacgtc gccgcggccc gtgccgaaca cgccggtgtc gaggtcgtcg
     2101 tgcaccccga gtgtgcgcac gacgtcgtgg agctcgcgga ccgcgtcggg tcgaccgagc
     2161 gcatccaggc atgggtgcag gacgcgccgg ccggcacggt catggcgatc ggcaccgaga
     2221 tccacatggt gcagcgcctc gccgcgcagc accccgacaa aacgatcgtg tcgctcgacc
     2281 cgttgatctg tccgtgctcg acgatgttcc gcatcgacgc gccgcacctc gcgtgggtgc
     2341 tcgagcggct cgtcgccggc gaggtcgtga atcagatcgt cgtcgacgac gacacatccg
     2401 agtgggcgca cgtcgcgctc caacggatgc tcgacatcac ctgagcttcc gcgcgacgaa
     2461 gaggagcagg cgcgggatcg ttgctgcggc gcggtactcc tctgcccggg cgaggaagcc
     2521 ctcgggcggc atcggttctt ccatgcgctc gatgagcagg ccctgcttcg ccatcgcatt
     2581 cacgtactgc gacagcggcc ggtgcacgaa cggcagcacg acgccaggcg ccagctcttc
     2641 catcgtgaca tcgggctcca ggtacgggcc gatccgccag tactgctcgt cgaggatgtg
     2701 gtcgacgacc cagccactgt tcggcgcctg caacagcggg tgattgagga agaacacgaa
     2761 ctgtccgccc ggcgcgagca cccgcgccac ttccgcgatc gcgggctcgt ggtcggggat
     2821 gtgctcgaac accaaacatg cgagcgcggt gtcgaagccg gcatcggcga acggcatcgc
     2881 ctccgcgaga ccctgcgcgt aggccggacc tcccccacgt tcccgggcga cccgcagctg
     2941 cgcgctcgtc gggtcgagac ccacgacgac gtcggccccg agatcgaccg cgacccgcgc
     3001 cagttgcccc tcacccgtcc cgacgtcgac gacgcgtgac gcgcccgcga ggcactcgcg
     3061 cgcgagcggg atgatctgct cctcgtattc gggatcggcg ccgtcggtga agccgtcctg
     3121 ccaccactgc gcgttgcgtt cccagtcggc gcggtcgata tcgctcaaga atcgatgccg
     3181 agcaacggcg cgaggaaacg gcccgtgtgg ctctcccgga ccttcgcgat ctgctccggc
     3241 gttccctcgg cgacgacggt gccgccgccg gtgccgccct cggggccgag gtcgatgacc
     3301 cagtcggcgc tctttatcac gtccaggttg tgctcgatca cgatcaccgt gttgcccttg
     3361 tcgaccagcc cctgcagcac gccgaggagc ttgctgatgt cctcgaagtg caggccggtg
     3421 gtcggctcgt cgaggatgta gatcgtgcgc cccgtcgagc gcttccccag ctcggtcgac
     3481 agcttcacgc gctgcgcctc accgcccgac aacgtcggcg cgggctgacc gagccgcacg
     3541 tagccgagac cgacgtcgac cagcgtctgc aactgacgcg cgatcgtcgg ctggttcgtg
     3601 aagaactcga gcgcctcctc ggccggcatg tcgagcactt cggcgatgtt cttgcccttg
     3661 aacgtgatgt cgagcgtgtc gcggttgtaa cgcgcgccct tgcacacctc gcacggcacg
     3721 tagacgtcgg ggaggaagtg catctcgatc ttgatcgtgc cgtcgccctg gcacgcctcg
     3781 cagcggccac ccttcacgtt gaaggagaac cggccctgct ggtagccgcg caccttcgcc
     3841 tcgggcgtgg tcgcgaacag gttgcggatc ttgtcgaaca cgcccgtgta cgtcgccggg
     3901 ttcgagcgcg gcgtgcggcc gatcggcgac tggtcgatgt tgatgacctt gtcgagttgg
     3961 ttcgcaccct cgatcttctt gtgccggccc ggcacctcgc gagaccggta gatcttctgc
     4021 atcaacgccg ggtgcaggat gtcgccgacg agcgtgctct tgcccgaacc gctcacgccc
     4081 gtcaccgcga cgaagcagcc gagcgggatc tcgacgtcga tgttctgcag gttgtgctca
     4141 cgcgcgccgc gcaccgcgat ccacgcgtcg ccgggctcgc gccgcatctc gggcaccggg
     4201 atcgaccgct tgcccgagag gtactgcccg gtgatcgacg ccttcgtctt gaggatgtct
     4261 ttcagcgagc cctcggccac gatcgcgccg ccgtgctcgc ccgcgccggg cccgatgtcg
     4321 acgatgtggt cggcgacgcg gatcgtctcc tcgtcgtgtt cgaccacgat caccgtgttg
     4381 ccgaggttgc gcagccgcat caacgtctcg atgagccgct tgttgtcgcg ctggtgcagc
     4441 ccgatcgacg gctcgtcgag cacgtacagc acgccgacca ggccgctgcc gatctgcgac
     4501 gcgagccgga tgcgttgcgc ctcgccgccg gcgagggtgc ccgacgaccg gttcaacgac
     4561 aggtagtcga ggccgacgtc cagcatgaac tggatccgct cgacgacctc tttgaacacg
     4621 cgctcggcga tcatccggtc gcgctccgac agctcgaccg ttcggaagaa gtcggccgcg
     4681 tcgccgatcg acatctcgcc gacctcgaac aggttcttgc cgccgaccgt caccgcgagg
     4741 gacgcgggct tcagccgcgc gccgccgcac gcgggacacg gcacgaggcg catgtagccc
     4801 tcgatctgct cgcgcacgcg gtcggagtcg gattccgcgt ggcgacgctc gacccacggg
     4861 atcacgcctt cgtacttcgc ggtgtactgc cgctggcgcc cgtatcggtt cttgtagcga
     4921 acggtgacgg tcttgccgcc ggtgccgtac agcacgatct tcttctgcgc ggccttgagc
     4981 ttcttccacg gtgtgtcgac cgagaagccg ttgtcggcgc cgaccgcctg caacacgcgc
     5041 gagaagtact cgccccgata accggaccag ggcgcgatcg cgccttccgc gatcgacagg
     5101 tcgtcgtcgg cgatcacgag ctcaccgtcg acctggaact tcgtgccgag gccgtcgcag
     5161 gtctcgcacg caccgtacgg cgagttgaac gagaagttgc ggggggccag ctcgtcgtac
     5221 gacaacccgc agtgtgtgca cgcgaggtgc tccgagaacg tgagcgtctc ccaccccccg
     5281 accccgccca ccgccccgct tggctgcgcc ttcggcgctt cttcgccctc acggggaacg
     5341 atctcgacct cggcgacacc ttccgcgatc ttcaacgccg tctcgagcga gtcggtcagc
     5401 cgccgctcga tgttggcccg gcgcaccagc cggtc
//
LOCUS       DASUGA010000881         3430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_76641, whole genome shotgun sequence.
ACCESSION   DASUGA010000881 DASUGA010000000
VERSION     DASUGA010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76641"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1001
                     /locus_tag="VFX21_17400"
     CDS             135..1001
                     /locus_tag="VFX21_17400"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX5097802.1"
                     /translation="MKAQVIGTGIPFAEGPVWCGDGTVVVTSVAAGALFRVDPARGSV
                     TKFAETSGGANGAALADDGSILVTQNGGIDFSALPLPDPLPACVPATPGLQLARPDGT
                     VTYLADTGFHGPNDLAITEAGDVFFTDPGHYPPATDDGGRVMVLARDGTVSEYARGFH
                     YCNGIVFDLDGHVVVVERTGLQRVFPDGSREWVVEKLGTGGGDGFCIDEDGRYYVAST
                     IEHGIRVIDTDGSELDFLPIDGKGVTTNCCFGGDDLRTLFVADALPGNLVAFEGMPTP
                     GLPLPTWPGLLG"
     gene            complement(1032..1664)
                     /locus_tag="VFX21_17405"
     CDS             complement(1032..1664)
                     /locus_tag="VFX21_17405"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX5097803.1"
                     /translation="MTRRPLGVVDIVYAARGDEVARAQAAAADGYAHIDPLLGTDPAT
                     LALPIGCPTAFPKPEPAWCSTPAPAATLDGAWERAVRWWTRAPQALMEPWAGACVNSL
                     DAIRAFKREVPGLRLLVDTGHVADWGEDPVELLEFADHVQLRQGKPGHTQLHIDDASG
                     VVDFMAVFQRLDQLDYQGKVSVEYFDLPEQSWSLEDPEQWALDLTAAVRH"
     gene            complement(1661..2551)
                     /locus_tag="VFX21_17410"
     CDS             complement(1661..2551)
                     /locus_tag="VFX21_17410"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5097804.1"
                     /translation="MGLLDGRVAIVTGSGRGIGREFALCFAREGAKVVINDVGVSLDG
                     QGTDDDPAAQVCKEIEALGSEAVPNYDSVADFEGARNIVKTATDAFGTVDILVNNAGI
                     VRDKTLLKMDESDFDSVIAVHLKGSFNTTRHAAEVMKEKGYGRIINITSSAGLRGNFG
                     QTNYGAAKAGLMGMTFIWSMELGRYGITCNAVAPAGATRMTAALYEKSGKEPPPEEDP
                     ALNAPLVAYLASEAAADVNGQILGRTEFAYTLFQHPKQIAWMWNDGPWTPQLVAERFD
                     KGLGQHLQQVGMVMPKSMTK"
     gene            complement(2552..2968)
                     /locus_tag="VFX21_17415"
     CDS             complement(2552..2968)
                     /locus_tag="VFX21_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX5097805.1"
                     /translation="MTAISYDAVSVGEERTQEFPALTRTMFVKYAGASGDFNPMHHDD
                     TIASQVGNPSVFGHGMLTMGLAARVVKDWFGPEAIRKFQVRFAKQVWPGDVLTCTAKV
                     TGKREENGEQVVDLDVSVVNQDGTQAITGSATAAVA"
     gene            3064..>3430
                     /locus_tag="VFX21_17420"
     CDS             3064..>3430
                     /locus_tag="VFX21_17420"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5097806.1"
                     /translation="MPRAAGERATRVAAENPRTLRSRGRNTRRKLLDAGAEVFASKGY
                     HATRVDDIVKVAQTSHGTFYLYFANKEELFSALAEEVATAMQELAESLAPLRPGADGE
                     AELRVWLEKFADLYETSGAI"
BASE COUNT          533 a         1197 c         1117 g          583 t
ORIGIN      
        1 gtgcgatacc ggcccccacg accgttcgtc ccccttccca ccccagaagg acgtgcggtt
       61 ctggcatcgg tcgtgatggc ggtcgaactt gaggaattgg ccgaacctgt tgacggcgtt
      121 tacggtcgca gatcgtgaaa gcgcaggtca tcggtactgg aattccgttc gcagaaggac
      181 cggtctggtg cggcgacggc accgtcgtgg tgacgagtgt cgcggcgggc gcactgtttc
      241 gcgtcgaccc cgcgcgtggg tcggtgacga agttcgccga aacctcgggc ggcgcgaacg
      301 gcgccgcgtt ggccgacgac ggctcgatcc tggtgacgca aaacggcggg atcgacttct
      361 ccgcgctccc gctccccgat ccgctgccgg cgtgcgtgcc ggcgacaccc ggactgcaac
      421 tcgcgcggcc ggacgggacc gtgacgtatc tcgccgacac gggctttcac ggcccaaacg
      481 atctcgcgat cacggaggcg ggcgacgtgt tcttcacgga ccccggccac tacccgcccg
      541 ccaccgacga cggcgggcgc gtcatggtgc tcgcacgcga cggcacggtg tcggagtacg
      601 cacgggggtt ccactactgc aacggcatcg tgttcgatct cgacgggcac gtcgttgtcg
      661 tagagcgcac gggcttgcag cgcgtctttc ccgacggctc gcgggagtgg gtcgtcgaga
      721 agctgggcac cggcggtggc gatggcttct gcatcgacga ggacggccgg tactacgtcg
      781 cgtcgacgat cgagcacggc attcgcgtga tcgacaccga cggcagcgag ctcgacttcc
      841 tgccgatcga cggcaaaggt gtcacgacca actgctgctt cgggggcgac gatctgcgga
      901 cgctgttcgt tgccgacgcg ctgcccggca atctcgtcgc gttcgagggc atgcccacac
      961 ccgggctgcc gctacccacc tggcccggcc tcctcggctg agggcgacac cgacgggcga
     1021 agaattctcg ctcagtgacg cacggctgcg gtgaggtcga gggcccattg ctcggggtct
     1081 tccaacgacc agctctgttc ggggaggtcg aagtattcga cgctcacctt gccctggtag
     1141 tcgagctggt ccaggcgttg gaagaccgcc atgaaatcga ccacgccgct cgcgtcgtcg
     1201 atatgcagtt gggtatggcc gggcttgccc tggcggagct gcacgtgatc ggcaaactcg
     1261 aggagctcga cgggatcttc gccccaatcg gcgacgtggc cggtgtcgac gagcaaccgc
     1321 aggcccggca cttctcgttt gaacgcgcgg atcgcgtcga gcgagttgac gcacgcgccc
     1381 gcccacggct ccatcaacgc ctgcggcgcg cgcgtccacc agcgcaccgc gcgctcccac
     1441 gcgccgtcga gcgttgccgc cggcgccggc gtggagcacc acgccggttc cggcttcggg
     1501 aacgcggtcg ggcacccgat cggcagtgcc agcgttgccg ggtcggtacc cagcagcgga
     1561 tcgatgtgcg cgtacccgtc ggcggcggcc gcctgcgcgc gcgcaacctc gtcgccgcgc
     1621 gccgcataga cgatgtcgac caccccgagc gggcggcgcg tcatttcgtc atggacttgg
     1681 gcatcaccat gccgacttgc tgcaagtgct ggccgagacc cttgtcgaag cgttcggcga
     1741 cgagctgcgg cgtccacggc ccgtcgttcc acatccacgc gatctgcttc ggatgctgga
     1801 acaacgtgta cgcgaactcg gtccggccga gtatctggcc gttcacgtcg gccgccgcct
     1861 ccgacgcgag atacgcgacg agcggcgcgt tcaacgcggg gtcttcctcg ggcggcggct
     1921 ccttgcccga cttctcgtag agcgcggcag tcatgcgcgt cgcccctgcc ggcgcaaccg
     1981 cgttgcacgt aatgccgtag cggccgagct ccatcgacca gatgaacgtc attcccatca
     2041 ggccggcctt ggccgcaccg tagttcgtct gcccaaagtt gccgcgcagt cctgccgacg
     2101 acgtgatgtt gatgatgcgg ccgtagccct tctctttcat cacctcggcc gcgtggcgcg
     2161 tggtgttgaa cgagcccttc aagtgcaccg cgatcacaga gtcgaaatcg gactcgtcca
     2221 tcttcaacag cgtcttgtcg cgcacgatcc cggcgttgtt caccaggatg tcgacggtgc
     2281 cgaacgcgtc ggtggcggtc ttcacgatgt tgcgcgcgcc ttcgaagtcg gccaccgagt
     2341 cgtagttcgg cacggcctcc gagccgagcg cttcgatctc cttgcaaact tgggccgcgg
     2401 gatcgtcatc ggtcccctgc ccgtcgagcg acacaccgac gtcgttgatg acgaccttgg
     2461 cgccttcgcg cgcgaagcac agcgcgaact ctcggccgat gccgcgtccc gatccggtga
     2521 cgatcgcgac gcggccgtcg agcagaccca tctacgcgac cgccgcggtc gccgaaccgg
     2581 tgatggcctg ggtgccgtcc tggttcacga ccgacacgtc gagatcgacg acctgctcgc
     2641 cgttctcctc gcgcttgccg gtgaccttgg cggtgcacgt gagcacgtcg ccgggccaca
     2701 cctgcttcgc gaagcgcacc tggaacttgc ggatcgcctc cgggccgaac cagtccttca
     2761 ccacccgggc cgccaagccc atcgtgagca tgccgtggcc gaacaccgac gggttgccca
     2821 cttggctggc gatcgtgtcg tcgtggtgca tcgggttgaa gtcgccgctc gctcccgcgt
     2881 acttcacgaa catggtccgg gtcagcgccg gaaattcctg cgtgcgctcc tcgcccacac
     2941 tcacggcgtc gtagctgatc gcggtcaacg ttccccctcg aatccacacc ttgcgaaccg
     3001 cccggacact agcggttcgc gctgaaacat gacaccggcg tcactgtccc ttagcctgac
     3061 cacgtgcccc gggccgccgg agagcgagcg acccgcgtcg ccgccgagaa ccctcgaacg
     3121 ctgcgctcgc gcggccgcaa cacgcgtcgc aagctgctcg acgccggcgc ggaagtgttc
     3181 gcgagcaagg gctaccacgc cacccgcgtc gacgacatcg tcaaggtcgc ccagacgtcg
     3241 cacggaacgt tctacctcta cttcgcgaac aaagaagagc tcttctccgc gcttgccgaa
     3301 gaggtcgcga cggcgatgca ggagctcgcc gaatcgctcg cgccgctgcg gcccggcgcc
     3361 gacggtgaag ccgagctgcg agtctggctg gagaagttcg ccgacctcta cgagacgtcg
     3421 ggggcgatct
//
LOCUS       DASUGA010000882         4534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1086387, whole genome shotgun sequence.
ACCESSION   DASUGA010000882 DASUGA010000000
VERSION     DASUGA010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086387"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VFX21_17425"
     CDS             complement(<1..590)
                     /locus_tag="VFX21_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fuconate dehydratase"
                     /protein_id="HEX5097807.1"
                     /translation="MTRITGARTLDVRAPTSRTLAGSDAVHQDPDYSGAYVFLATDGP
                     HEGVGMTFTIGRGNDLCCAAIEQLAPLVVGRELEELAADTAALWRRLTNDGQLRWLGP
                     EKGVVHLATAALVNAVWDLWAKVEGKPLWRLVCDLTPEQLVTAVDFRYLTDAVDRDQA
                     LDILRAQAPTRAARIAALERDGYPAYITSAGWLGYD"
     gene            639..1346
                     /locus_tag="VFX21_17430"
     CDS             639..1346
                     /locus_tag="VFX21_17430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5097808.1"
                     /translation="MSERVVIVTGSRGGIGTAIAERFGASGDVVVGLDLLDGFDVSDP
                     DACATTVRGIVDEHGGIDVLCNNAGIGAVGDAVTATPDDWRRVFAVNVFGVANMCRAV
                     LPVMRAAGRGAVVNTCSVVASVGLVERAVYSASKGAVLAFTKSLAADEVGYGIRVNCV
                     SPGTVLSPWVERLAQAQDDPEQVLTALRRRQPLGRMVSCEEVADAVHYLAADTTFTSG
                     ADFLLDGGITGMRLLES"
     gene            1343..2437
                     /locus_tag="VFX21_17435"
     CDS             1343..2437
                     /locus_tag="VFX21_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5097809.1"
                     /translation="MTLRVALVGGPMYDHLYESFAPGEVEVVVHADHPTLNRRIAELL
                     AAGERIDVLATHAKYSPSQAQWLRPLDDLLDPAALAPLAPAAVAACRNGSALLCAPRL
                     IDVRVLWVRADRVAHVPDSWAQLLDSEVVFGFPGRESGLFGTFFELVVGRGGRLFDDD
                     QRPTMATPEAEAAVETLCALAARAPGDLVGWHYDDVDRALLDGRVDAAAAWPGGFGAI
                     RASALAAQLRPHPYLAGPVRRVSYSGCHAWAIPRTCGDVDGAVALVTRLLGAQAQGVD
                     AAGGNMCAHEAALAAFAAGAEDPVDRERLAITGATIRDAMIGYPQLSRFPEVEDAGWS
                     AIRDALCGQCTPVAATHAIQQAAEKVLASE"
     gene            2442..3188
                     /locus_tag="VFX21_17440"
     CDS             2442..3188
                     /locus_tag="VFX21_17440"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5097810.1"
                     /translation="MPDLAIAAESVTIAGANGDEIEAYFARPTGAGASPGVVVIHHMP
                     GFDRASKEITRTFAAYGYSALCPNLHHRYAPGAKATEAAAAAMEAGGVPDDQCIGDIG
                     GAVAFLRTLPNANGKVGVIGYCSGGRQAYVVACSLDIDAAVDCYGGRVVATPDDLTPA
                     RPISPVDRTPDLRCPLLGLFGAEDSNPSPEQVTQIEAALRAHGKTYEFYTFENAGHAF
                     FAVDRPNYRVDAAREGWKIIWDFFGRYLAA"
     gene            complement(3338..>4534)
                     /locus_tag="VFX21_17445"
     CDS             complement(3338..>4534)
                     /locus_tag="VFX21_17445"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX5097811.1"
                     /translation="RAGGPALGDRYVVANGAEGEPATFKDRPLLRSNPYQVLEGLAVA
                     CTIVGAREAFCAVKASFAPEIDALQRALREMADANLICDAPVSLVTGPEEYLFGEEKA
                     LLEVIEGNDPMPRWLPPYLHGLYATTPQEGWSASGPPTDVEEADIVGANPTLVTNVET
                     LANVPLILTRGADWYRTIGTAETPGPFLCTVVGDVVHAGTAEIEPGVTLREVIDRIGG
                     GVHPGRQVKAVLSGVSNPALSAAQLDAPVSYEGLDAAGGGLGSAGFLVYDDTRSMAAV
                     AQMVSRFLYVESCGQCRACKFGCGEITRTLDRLAATEGERRDFDLIGERLLGVTDQNR
                     CFLGAEEQRVVSSLVRLFPEDFAAQLEGAPPLDALPIPKILDIRDGVAIYDEKQMRKQ
                     PDWTYA"
BASE COUNT          641 a         1583 c         1613 g          697 t
ORIGIN      
        1 tcgtcgtagc cgagccagcc cgcggacgtg atgtacgcgg ggtacccgtc gcgttcgagc
       61 gccgcgatgc gggccgcgcg cgtcggcgcc tgcgcgcgga ggatgtcgag cgcctgatcg
      121 cggtcgaccg cgtcggtgag ataacggaag tcgacggccg tcacgagctg ctcgggcgtg
      181 agatcgcaga cgagccgcca caacggcttg ccttcgacct tcgcccagag gtcccatacc
      241 gcgttgacga gcgccgcggt cgcgaggtgg acgacgccct tctcgggccc gagccaacgc
      301 agttggccgt cgttggtcag gcgccgccac agtgcggcag tgtcggccgc cagctcctcg
      361 agctcgcgcc cgacgacgag cggcgccagc tgctcgatcg ccgcacagca caggtcgttg
      421 ccccggccga tcgtgaacgt catgccgacg ccttcgtggg gaccgtcggt ggcgaggaac
      481 acgtacgcgc ccgagtaatc gggatcctgg tgcaccgcgt cggagccggc cagcgtgcga
      541 gacgtcggcg cgcgcacgtc gagcgtgcgg gcgccggtga tgcgcgtcat tggcgcggat
      601 cttagacacg cgtttcggcg gtgcgtcatg atgcggcgat gagcgaacga gtcgtgatcg
      661 tcaccggttc gcgcggcggc atcgggaccg cgatcgccga gcgcttcggc gccagtggcg
      721 acgtggtcgt gggcctcgat ctcctcgacg gcttcgacgt cagcgatccc gacgcgtgtg
      781 cgacgaccgt gcgcgggatc gtcgacgagc acggtggcat cgacgtgctg tgcaacaacg
      841 ccggcatcgg cgcggtcggc gacgcggtga ccgcgacgcc cgacgactgg cggcgcgtgt
      901 tcgcggtgaa cgtcttcggt gtcgccaaca tgtgccgggc cgtgctgccg gtcatgcgcg
      961 ccgcgggacg cggcgccgtg gtcaacacgt gctcggtcgt tgcgtcggtc gggctcgtcg
     1021 aacgggccgt ctactccgcg tcgaagggcg cagtgctcgc gttcacgaag tcgctcgccg
     1081 ccgacgaggt cggctacggc atccgcgtca actgcgtctc gcccggcacc gtgctcagcc
     1141 cgtgggtcga acggctcgcg caggcgcagg acgatcccga gcaggtgctc accgcgctgc
     1201 gccgccgcca accgctcggc cgcatggtgt cgtgcgaaga agtcgcggac gcggtgcact
     1261 acctcgcggc cgacacgacg ttcacgagcg gcgcggactt cctcctcgac ggtggcatca
     1321 cgggcatgcg cctcctcgag tcatgacgct gcgcgtcgcg ctcgtcggtg gcccgatgta
     1381 cgaccacctc tacgagtcgt tcgcgcccgg cgaggtggag gtggtcgtcc acgccgacca
     1441 cccgacgttg aaccggcgca ttgcggagct gctggcggcg ggtgagcgca tcgacgtgct
     1501 cgcgacacac gccaagtact cgccgtcgca ggcgcagtgg ctccggccgc tcgacgatct
     1561 gctcgacccg gcggcactcg cgccgctcgc gcccgccgcg gtggcggcgt gtcgcaacgg
     1621 ctccgcgctg ttgtgcgcgc cgcgcttgat cgacgtgcgc gtgctgtggg tacgcgctga
     1681 ccgcgttgcg cacgtacctg actcgtgggc gcagctgctc gactccgagg tcgtcttcgg
     1741 ctttcccggc cgggagtcgg gactgttcgg cacgttcttc gaactcgtcg tcgggcgcgg
     1801 cggtcgtctc ttcgacgacg accaacgacc gacgatggcc acgcccgaag ccgaggccgc
     1861 cgtcgagacg ctgtgtgcgt tggccgcccg ggcgccgggc gatctcgtcg ggtggcacta
     1921 cgacgacgtc gaccgcgcgt tgctcgacgg tcgcgtcgac gcggccgcgg cgtggcccgg
     1981 tggcttcggt gcgatccgcg cgtccgcgct cgccgcgcaa ctgcgcccac atccgtatct
     2041 cgcggggccg gtgcggcgcg tctcgtactc cggttgtcac gcgtgggcga tccctcgcac
     2101 gtgcggcgac gtcgacggtg ccgttgcgct cgtgacgcgt ttgctcggtg cgcaggcaca
     2161 gggcgtcgac gcggccggcg gcaacatgtg tgcgcacgag gcggcgctcg ccgcgttcgc
     2221 ggcgggggcc gaagatcctg tcgatcgtga gcggctcgcg atcacgggcg cgacgatccg
     2281 cgacgcgatg atcggctatc cgcagctgtc gcgctttccc gaggtagagg atgccggctg
     2341 gtcggcgatc cgcgacgcgc tgtgcgggca atgcacccca gttgcggcaa cccacgcgat
     2401 ccagcaggcc gccgagaagg tgctcgcgtc agaatgaagc catgccggac cttgcgatag
     2461 cagccgagag cgtcacgatc gccggcgcga acggcgacga gatcgaggcc tacttcgccc
     2521 ggccgaccgg tgccggtgcg tctccgggtg tcgtcgtgat ccaccacatg ccgggtttcg
     2581 accgagcctc gaaggagatc acgcgcacgt tcgcggccta cggctacagc gcactgtgcc
     2641 cgaacctcca ccatcgctat gcccccggcg ccaaggcaac cgaggccgcg gccgcggcga
     2701 tggaagccgg cggcgtgccc gacgaccagt gcatcggtga catcggcggc gcggtggcgt
     2761 tcttgcgcac gttgccgaac gcgaacggca aggtcggcgt gatcggctac tgctccggcg
     2821 gccggcaggc ctacgtggtg gcgtgcagtc tcgacatcga cgccgcggtc gactgctacg
     2881 gcggtcgcgt cgtcgcgaca cccgacgacc tcacgcccgc gcgcccgatc tcgcccgtcg
     2941 accgcacgcc cgacctgcgc tgtccgctcc tcggcttgtt cggcgcggag gactcgaacc
     3001 cgtcgccgga acaggtgacc cagatcgagg cggccctgcg agcgcacggc aagacgtacg
     3061 agttctacac gttcgagaac gccgggcacg cgttcttcgc cgtcgaccga ccgaactatc
     3121 gcgtcgacgc cgcgagggaa ggctggaaga tcatctggga cttcttcggc cgctacctcg
     3181 cggcgtaacc gcgcgaccgg caagacagtg acgtgcgaac gttgtcaatc tggcccgcta
     3241 attcggggcc agattgacaa ggaaccacaa acgcgaatcc gacgccggcg atgaggtgcg
     3301 tgaccgttga tcgagacgta ttcacccatc ggtcacctca cgcgtacgtc cagtcgggct
     3361 gcttgcgcat ctgcttctcg tcgtagatcg caacgccgtc gcggatgtcg aggatcttcg
     3421 ggatcgggag cgcgtcgagc ggcggcgcac cttcgagttg cgccgcgaag tcttcgggga
     3481 acaatcgcac cagactcgag acgacgcgct gctcctcggc gccgaggaag caccggttct
     3541 ggtcggtgac gcccagtagc cgctcgccga tcaggtcgaa gtcgcgtcgc tcgccctcgg
     3601 tcgccgcgag gcggtcgagc gtgcgtgtga tctcgccgca gccgaacttg cacgcgcggc
     3661 actggccgca cgactcgacg tagaggaagc gcgacaccat ctgcgcgacc gcggccatgc
     3721 tgcgcgtgtc gtcgtagacg aggaagccgg ccgacccgag gccaccgccg gccgcgtcga
     3781 ggccctcgta gctcaccggc gcgtcgagct gcgcggccga cagcgcggga ttcgacacgc
     3841 ccgacagcac ggccttgacc tgccggcccg ggtgaacgcc gccgccgatg cggtcgatga
     3901 cctcgcgcag cgtgaccccc ggttcgatct cggcggtgcc cgcgtgcacc acgtcgccga
     3961 cgacggtgca caggaacggg cccggtgtct cggcggtgcc gatcgtgcgg taccagtcgg
     4021 cgccgcgggt caggatcaag ggcacgttgg cgagtgtctc gacgttcgtc accagtgtgg
     4081 ggttcgcgcc gacgatgtcg gcctcctcga cgtccgtcgg cgggccgctc gccgaccagc
     4141 cctcctgcgg cgtcgtcgcg tagagcccgt gcaagtacgg cggcaaccag cgcggcatcg
     4201 ggtcgttgcc ctcgatgact tcgagcagcg ccttctcctc gccgaagagg tactcctcgg
     4261 gaccggtgac gagcgagacc ggcgcgtcgc agatgaggtt cgcgtctgcc atctcgcgca
     4321 acgcgcgctg cagcgcgtcg atctcgggcg cgaacgacgc cttgaccgca cagaacgctt
     4381 cgcgggcgcc gacgatcgtg cacgcgaccg cgagcccctc gaggacctgg tacggattcg
     4441 accggaggag cggccggtcc ttgaacgtgg ccggctcgcc ctccgcgccg ttggcgacga
     4501 cgtagcggtc gccgagagcc gggccgcccg cgcg
//
LOCUS       DASUGA010000883         7109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1189275, whole genome shotgun sequence.
ACCESSION   DASUGA010000883 DASUGA010000000
VERSION     DASUGA010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7109
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189275"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VFX21_17450"
     CDS             complement(<1..939)
                     /locus_tag="VFX21_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004953100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097812.1"
                     /translation="MRALRSLLCAAGMVLLVAAPAASAPTSSDPPTAADAAARWLVDQ
                     MRTDGFLPSAFVPGTPDMGSTAQAIPALAAAGVGGAQAQTMLDALGAQVEDFVVIGGV
                     DNPGNLSYLILAATALGDDPTSFGTGNTNLVARLQALQTPSGLYGSADPSFDGAFRQG
                     LALMALDAAGVADPDGANWLADQQCADGSWTPFRTDTSVPCPPVDPVNFTGPDTNSTA
                     LALLGLQAAGVNSPLADGVTALDNVRTSEGGWGFLADSGQATDANSTGLVVEAMHAVN
                     GGSDAQGTAALLALQSGCDADPLDRGGIAFQPGPGGS"
     regulatory      complement(1027..1158)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1291..2805
                     /locus_tag="VFX21_17455"
     CDS             1291..2805
                     /locus_tag="VFX21_17455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5097813.1"
                     /translation="MAAVAVMMASACSSGDDDDAAADSDETTASTAAGETTPTTAVEI
                     DRSGRFAAEDTFCTPATEETETEAPEQIDDGITEDEISITHIRVKLEELANLGFAVDI
                     GDTNDYVKTFVDIVNDRCGGIHGRKIKLNTVEVPALPGEGQDTSSVAQAACIEAAEDQ
                     HAAFAFSTSGFGDVGVPCLTQSHEVVFITSYTVSLDDIDKAESRLYSVGLAGEESLTY
                     AARDLAAQGLLDGKKIGVVRPDGTPDAQIVERGLVDVLEDELGLDVVRVDTIGCGGTN
                     SCTEGVADSVQGMIADGVDVLFPALNVISLPGYLAEMTNQGVQPGDITFYQTGYLAQS
                     GDLVSGKVVEFGGEAAGNLYNGTTVIASGPTGEFRLDGYEPAPFTQMCNKEYAENGAN
                     SEGEYDATNEATATKLGALVGVCSDIRMIARAIETAGANPSRASLAEAMENLGAVDQG
                     GRDRYGSFAPGKYTAPNTLFTNIFHYPCPYETTAKSGACIVVDGDARPLPTEGS"
     gene            2802..5006
                     /locus_tag="VFX21_17460"
     CDS             2802..5006
                     /locus_tag="VFX21_17460"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5097814.1"
                     /translation="MTQGDPAALTAAVLDEESRRQAVQDERSAVTILPDDLLPGVGGE
                     QLGLRETLKVGGTATIAVIFAMIVVEQFSRVGLGVLGPDIQTTLDVSDTTLVAIAAFS
                     GVALVLGAVPVAWLADRMPRKYVVVGSTLFGGVALLVTGVVVNPFQMFWALAAVSLSY
                     AYAIPVFGSLMADQYPINGRSRIFALFFMAQPLGALIAPFLVGLIADRAGGTEGWRWV
                     YLIVAIPTFVLAVIAAFVLREPTRGKYEQQLILGGELEHTDVRKELPVSISNAYQRMK
                     KIKTFYYICVGIGVLGFAFVAVPVQLGLLLRDHYGYGPYARGWITSVGFVASLIAIPI
                     AGFAFDRAFRKNPERVVRIAGGLIFGYGALMLIGLRMKEIVLLMLFVSLANACTGAAF
                     VGIGPIIGGVAPYRLRSQAFALVPVFIFLVGGFFGGLITGALSDAHGERTALTIVIPF
                     AAGIGGALFVHGARYLKRDISLAVEELLEEQAELQRMSAAPESIPMLQVRNLDYSYGP
                     VQVLFDVGFEVQKGEVLALLGTNGAGKSTLLRAISGLGIPDRGVVRLDGQTLTYVDAE
                     LRFRAGIVQLRGGAGVFPELTVRDNLKASLLSTKMRPAELRARIERISELFPILRERM
                     EQPARDLSGGQQQMLALALALMHDPQVLLVDELSLGLAPLVVQELLGVVEELRAQGLT
                     IVIVEQSLNVALAVADRAIFMEKGRVRFEGSARELAERDDLARAVFLGGEGG"
     gene            5016..>7109
                     /locus_tag="VFX21_17465"
     CDS             5016..>7109
                     /locus_tag="VFX21_17465"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5097815.1"
                     /translation="MIAALDIEITQQVLFSGIVTGLVYAVLAAGFVLVYRSTGVLNFA
                     QGEIGAFGVALFTLLQAGYGVPYWLAFAMAVACSAAIGTVTEMIVVRRLFNAPRLVLL
                     IATLGVAQLLLVARLMLPNTAASKQFPLPFHGIWHPTAELTVLAREVLVLVVAPVAII
                     GLALFMTHTKLGLLVRASASNPDTARVYGISVRRTSTIVWTIAGAFAGLTAILYAPIR
                     GITPGTIIGAGAVAIGPSLLLRALVVSLIARLRSLPMCLVGGLAVGVAESIVIASNGG
                     DRTIVELYLFLATLALVLFTNRSVRPDANWTLTSNVKPVPERLRSLWYVRHLSKLGYA
                     VLFGSLAILPFFLSKRSQESLWTDVLLFAMAALPISMLIGWAGQVSLGQFAFVGLGAC
                     TMVVLTHDLDIPVPFDLWTMHFELSWLPALVISTAVGVVLALLMGLPALRARGLMLAV
                     VTFAFAVAATQWLFAQSVWTGDEFATNMPSMEKPELLGIDFSNRRSFYYLCLACLFLM
                     AMMTARLRRTGIGRSMIAVRDNEEMAAASTVSATRMKLSAFAISGGMAAFSGCLFVTA
                     REFVQPTSTFDPDSSLRLVSTAIIGGLGSVAGPIIGALFVRGLPTIFDDVSEVQLATS
                     GIGLLILLMYFPGGLMQILYALRNAILGWAERRLPAAEPRAVPAAPAALTRDRPAHAV
                     SLATPGAAWLSVSHVT"
BASE COUNT         1017 a         2428 c         2475 g         1189 t
ORIGIN      
        1 cgaaccgccg ggccccggtt ggaacgcgat gccgccgcga tcgagcgggt cggcatcgca
       61 gcccgactgc aacgcgagca gcgcagccgt gccttgcgcg tcgcttccgc cgttcaccgc
      121 gtgcatcgcc tcgacgacca agcccgtcga gttggcatcg gtggcctgac cactgtccgc
      181 gaggaagccc caaccgccct cgctcgtgcg cacgttgtcc agcgcggtca cgccgtccgc
      241 gagcgggctg ttcacgcccg cggcctgcag accgagcagc gcgagcgcgg tcgagttcgt
      301 gtccggcccg gtgaagttga ccgggtccac cggtgggcac ggcaccgacg tgtcggtacg
      361 gaatggtgtc cacgaaccgt cggcgcactg ctggtccgcg agccagttcg caccgtccgg
      421 gtccgccacg cccgcggcgt cgagcgccat gagcgcgagg ccttgacgga acgcaccgtc
      481 gaagctcggg tcggccgagc cgtacaagcc gctcggcgtc tgcaacgcct gcaagcgcgc
      541 gaccaggttc gtgttgccgg tgccgaacga cgtcggatcg tcgccgaggg cggtcgccgc
      601 gaggatcagg tacgacaagt tcccggggtt gtccacgccg ccaatgacga cgaagtcctc
      661 gacctgcgcg ccgagggcgt cgagcatcgt ctgtgcttgc gcgccgccga ccccggccgc
      721 cgcgagcgca gggatcgcct gcgcggtaga tcccatgtcc ggggtgccgg gcacgaacgc
      781 ggacggcaag aacccgtcgg tccgcatttg gtcgacgagc caacgcgccg cggcatcggc
      841 ggcggtcggc gggtcgctgc tggtcggtgc gctcgcggct ggagcagcca cgagcaacac
      901 catcccggca gcgcagagaa gtgagcgcag tgcacgcatc aggcttcctt tcacgctgtg
      961 ccggggcgtc aggtgaaagg gccacggccg gagccgaggc gctcccgatc ccgcatccca
     1021 cgacggtcga gtggagctgc gccacacagg tgctccggct cgcagggacg aaccctgcct
     1081 acggttgcgg gtcagcgccg gacttcgacc ggcttccccc gttgtggtgc acaggggatg
     1141 caatcacgtc cacggaatct gcgccaatca ccatgccgtg acacggtgcc tccgctcgac
     1201 catgatggcg ccgacggaac gcgcgggttc gcgggacgcg actcgaaccg gggggacacg
     1261 gtgcgagcag gctggtggcg tatcggtgcg gtggctgcgg tcgccgtgat gatggcgagt
     1321 gcgtgctcga gcggcgacga cgacgatgcc gccgccgatt ccgacgagac gaccgcatcg
     1381 acggccgcgg gcgagacgac gccgacgacc gcggtcgaga tcgatcgcag cggtcgcttc
     1441 gcggccgagg acacgttctg cacgccggcg acggaggaaa ccgaaaccga ggcgcccgag
     1501 cagatcgatg acggcatcac cgaggacgag atctcgatca cgcacatccg agtgaagctc
     1561 gaagagctcg cgaacctcgg attcgccgtc gacatcggcg acacgaacga ctacgtcaag
     1621 acgttcgtcg acatcgtcaa cgaccgctgc ggcggcatcc atggccgcaa gatcaagctg
     1681 aacacggtcg aggtgcccgc gctgcccggt gagggtcagg acacgagctc ggtcgcgcaa
     1741 gccgcctgca tcgaagcggc cgaagaccag cacgccgcgt tcgcgttctc gacgagcggc
     1801 ttcggcgacg tcggcgtgcc gtgcctgacg cagagccacg aagtcgtgtt catcacgtcg
     1861 tacacggtga gtctcgacga catcgacaag gccgagagcc gcttgtactc cgtcgggctc
     1921 gcgggcgagg agtcgttgac gtacgcagcg cgcgacctcg cggctcaagg cctgctcgac
     1981 ggcaagaaga tcggtgtcgt gcggcccgac ggcacgcccg acgcgcagat cgtcgaacgt
     2041 ggcctcgtcg acgtgctcga ggacgagctc ggtctcgacg tcgtgcgcgt cgacaccatc
     2101 ggttgcggcg gcacgaactc gtgcaccgaa ggcgtcgccg attcggtgca gggcatgatc
     2161 gccgacggcg tcgacgtcct gttccccgca ttgaacgtca tctcgctgcc gggctacctc
     2221 gctgagatga cgaaccaggg tgtgcagccg ggcgacatca ccttctacca aacgggctat
     2281 ctcgcgcagt cgggtgacct cgtgtcgggg aaggtggtcg agttcggcgg cgaggccgcg
     2341 ggcaacctct acaacggcac gacggtcatc gcctcgggcc cgaccggcga gttccgcctc
     2401 gacggttacg aacccgcgcc gttcacgcag atgtgcaaca aggagtacgc cgagaacggc
     2461 gccaacagcg aaggcgagta cgacgccacc aacgaggcga ccgcgacgaa actgggcgcg
     2521 ctcgtcggtg tgtgctccga catccgcatg attgcccgcg cgatcgagac ggccggcgcg
     2581 aacccgtcac gcgccagcct tgcggaggcg atggagaatc tcggcgcggt cgaccaaggc
     2641 ggacgcgacc gctacggcag cttcgcgccg ggcaagtaca ccgcgccgaa cacgctcttc
     2701 acgaacatct tccactatcc gtgtccatac gagacgaccg ccaagtccgg cgcgtgcatc
     2761 gtcgtcgatg gagacgcgcg gccactcccc actgaaggct cgtgacgcaa ggggaccccg
     2821 cagccctgac tgcggcggtg ctcgacgagg aatcgcgccg gcaggcagtg caggacgaac
     2881 ggtcggcggt cacgatcctg cccgacgacc tgctgccggg cgtcggcggt gaacagctcg
     2941 gcctccggga gacgctgaag gtcggcggga ccgcgacgat cgcggtgatc ttcgcgatga
     3001 tcgtcgtcga gcagttctcg cgcgtcggtc tcggcgtcct cggccccgac atccagacga
     3061 cactcgacgt cagcgacacg acgctcgtcg cgatcgcggc gttcagcggt gtcgcgctgg
     3121 tgctcggcgc ggtgcccgtg gcgtggctcg ccgaccggat gccgcgcaag tacgtcgtcg
     3181 tcggctcgac gctgttcggc ggcgtcgcgc tgctcgtgac cggtgtcgtg gtgaacccgt
     3241 tccagatgtt ctgggcgctc gccgcggtgt cgctcagcta cgcctacgcg atccccgtgt
     3301 tcgggtcgct catggccgac cagtacccga tcaacggccg gagccggatc ttcgccctgt
     3361 tcttcatggc gcaaccgctc ggcgcgctga tcgcgccgtt cctcgtcggg ctcatcgcgg
     3421 atcgcgccgg tggcaccgag ggttggcggt gggtctacct gatcgtggcc atcccgacgt
     3481 tcgtgctcgc cgtcatcgcc gcgttcgtgc tgcgggagcc gacgcgcggc aagtacgagc
     3541 agcagctcat cctcggcggt gagctcgagc acaccgacgt ccgcaaggag ctgccggtct
     3601 cgatctcgaa tgcgtaccag cgcatgaaga agatcaagac gttctattac atctgtgtcg
     3661 gcatcggcgt gctgggcttc gcgttcgtcg cggtgcccgt gcagctcggc ctgttgctgc
     3721 gcgaccacta cggctacggg ccgtacgcgc gcggttggat cacctcggtt ggcttcgtcg
     3781 cgtcgctgat cgcgatcccg atcgccggtt tcgcgttcga ccgcgcgttc cgcaagaacc
     3841 ccgaacgcgt cgtgcgcatc gcgggtgggc tgatcttcgg ctacggcgcg ctgatgctca
     3901 tcgggctacg catgaaggag atcgtgctgc tgatgctgtt cgtgtcgctg gcgaacgcgt
     3961 gcacgggcgc cgcgttcgtc ggcatcggcc cgatcatcgg cggcgtcgcg ccgtaccggc
     4021 tgcgctcgca ggccttcgcg ctggtgcccg tcttcatctt tctcgtcggc ggtttcttcg
     4081 gcgggctcat caccggtgcg ctctccgacg cgcatggcga gcggaccgcg ctgacgatcg
     4141 tgatcccgtt cgcggccggc atcggcggcg cgctgttcgt gcacggcgcc cgctacctca
     4201 agcgcgacat ctcgctcgcg gtcgaagagc tgctcgagga gcaagcggag ctgcagcgca
     4261 tgagcgcggc accggagtcc atcccgatgc tccaggtgcg caacctcgac tacagctacg
     4321 gaccggtcca ggtgctcttc gacgtcgggt tcgaggtgca gaaaggcgag gtgctcgcgc
     4381 tgctcggcac gaacggcgcg gggaaatcga cgctgctgcg ggccatcagc gggctgggca
     4441 tccccgaccg gggtgtcgtg cggctcgacg gtcagacgct cacgtacgtc gacgcggagc
     4501 tgcggttccg ggccgggatc gtgcagctgc gcggcggcgc gggcgtgttc cccgagctga
     4561 ccgtgcgcga caacctgaag gcgtcgctgc tctcgacgaa gatgcggccg gccgagctgc
     4621 gggcgcgcat cgagcgcatc agcgaactgt tcccgatcct gcgcgagcgg atggagcagc
     4681 ccgcccgtga tctctcgggc ggccagcagc agatgctcgc cctcgcgctc gcgctgatgc
     4741 acgacccgca ggtgctcctc gtcgacgagc tgtcgctcgg gctggcgccg ctcgtcgtgc
     4801 aagaactgtt gggcgtcgtc gaggagctcc gcgcgcaggg cctgacgatc gtgatcgtcg
     4861 agcagtcgct gaacgtcgcg ctcgcagtcg cggaccgggc gatcttcatg gagaagggcc
     4921 gggtgcgctt cgagggctcg gcgcgcgagt tggccgagcg cgacgacctc gcccgggccg
     4981 tcttcctcgg cggcgaaggc gggtgagctg acccggtgat cgccgcgctc gacatcgaga
     5041 tcacccagca ggtgttgttc tccggcatcg tgaccgggct cgtgtacgcg gtgctcgccg
     5101 ccggcttcgt gctcgtctac cgctcgaccg gtgtcctcaa cttcgcgcag ggcgagatcg
     5161 gtgcgttcgg tgtggcgctg ttcacgctgc tccaggcggg ttacggcgtg ccgtactggt
     5221 tggcgttcgc gatggcggtc gcgtgcagcg ccgcgatcgg caccgtcacc gagatgatcg
     5281 tggtgcgacg cctgttcaac gcgccccgcc tggtgctcct gatcgcgacg ctcggcgtcg
     5341 cgcagctcct gctcgtcgcg cggctgatgc tgccgaacac ggcggcttcg aagcagttcc
     5401 cgctgccgtt ccacgggatc tggcacccga cggccgagct gaccgtgctc gcgcgcgagg
     5461 tgctggtgct ggtcgtcgcg ccggtcgcga tcatcgggct cgcgctgttc atgacgcaca
     5521 cgaagctcgg gctgctcgtc cgggcctcgg cgtccaaccc cgacaccgca cgcgtctacg
     5581 gcatcagtgt gaggcgcacg tcgacgatcg tgtggacgat cgccggtgcg ttcgccggtc
     5641 tcaccgccat cttgtacgcg ccgatccgcg gcatcacgcc gggcacgatc atcggcgccg
     5701 gcgcggtcgc catcggtccc agcctgttgc tgcgggcgct cgtcgtcagc ctcatcgcgc
     5761 gcttgcggtc gctgccgatg tgcctcgtcg gcggcctcgc ggtcggcgtc gcggagtcga
     5821 tcgtgatcgc gagcaacggg ggcgaccgga cgatcgtcga gttgtacctg ttcctcgcga
     5881 cgctcgcgct cgtgcttttc acgaaccgct cggtgcgacc ggatgcgaac tggacgctca
     5941 cgtcgaacgt gaaaccggtg cccgagcggc tgcgcagcct ctggtacgtg cggcacctgt
     6001 cgaagctcgg atacgccgtg ctgttcggca gcctcgcgat cctgccgttc ttcctgagca
     6061 agcggtcgca ggagtcgctg tggaccgacg tgctgctgtt cgcgatggcc gcgctgccga
     6121 tctcgatgct catcgggtgg gccgggcagg tgtcgttggg ccagttcgcg ttcgtcgggc
     6181 tcggcgcgtg cacgatggtc gtgctcaccc acgacctcga catccccgtg ccgttcgatc
     6241 tgtggacgat gcacttcgag ctgtcgtggc tgcccgcact ggtgatctcg acagcggtcg
     6301 gcgtggtgct cgcgctcctc atgggcctcc cggcgttgcg ggcgcgcggg ctcatgctcg
     6361 cggtcgtcac gttcgcattc gccgtcgccg cgacgcagtg gctgttcgcc cagtcggtgt
     6421 ggaccggcga cgagttcgcc acgaacatgc cctcgatgga gaagcccgag ctcctcggca
     6481 tcgacttctc caaccgccgc tcgttctatt acctgtgcct cgcctgcctg ttcctgatgg
     6541 cgatgatgac ggcgcggctg cggcgcaccg gcatcggccg ttcgatgatc gcggtgcgcg
     6601 acaacgagga gatggccgcg gcgtcgaccg tgtcggccac ccgcatgaag ctgtccgcgt
     6661 tcgcgatctc gggcggcatg gccgcgttct cgggttgttt gttcgtgacc gcccgcgagt
     6721 tcgtgcagcc gacgtctacg ttcgaccccg acagctcgct gcggctggtg tccacggcga
     6781 tcatcggcgg gctcgggtcg gtcgcggggc cgatcatcgg cgccctgttc gtgcgcggac
     6841 tgccgacgat cttcgacgac gtctccgagg tgcagctcgc gacgagcggc atcgggctgc
     6901 tcatcttgtt gatgtacttc ccgggcgggc tgatgcagat cctgtacgcc ctccgcaacg
     6961 cgatcctcgg gtgggccgag cggcggctgc ccgcggccga gccacgcgcg gtaccggccg
     7021 cgcccgccgc cctcacccgc gaccggcccg cgcacgcggt gtcgctggca acacccggcg
     7081 cggcgtggct gtcggtatca cacgtgacc
//
LOCUS       DASUGA010000884         2089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1987620, whole genome shotgun sequence.
ACCESSION   DASUGA010000884 DASUGA010000000
VERSION     DASUGA010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1987620"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="VFX21_17470"
     CDS             <1..1321
                     /locus_tag="VFX21_17470"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX5097816.1"
                     /translation="KQLVDETAAAFRETWDLLDIAYDDFIRTTEPRHYQAVQQFLQVL
                     YDNGAIELGTYEGLYCVSCEAYYTEDELIDGNCPIHGRPVEHVTEENYFFRLSRYEQR
                     LLDHYAAHPDAVQPDYRMNEVLGFIRQGLRDFSMSRTSISWGVPLPWDPKHVAYVWAD
                     ALFNYCTAVGYGADDARFARFWPVDYHLVGKDILRFHAVYWPAMLMAAGLEPPDCVYA
                     HGWLLVGGEKMSKTRLNQIAPADLVADFGVDGFRYHFMADQRFGPDGDFSYEAMVLRY
                     NADLANNFGNLANRVLNMAVNYVGGVVPDGRADGPLMGAATEAFDLVQGAMAKLDFAG
                     ALAAVWDLIRAANSYIEERQPWALHKAGNATEVAEVLGDCLEALRIVTLLASPVIPNA
                     TAELWRRLGLAGRPEDTRLPDAAKWGQLAPGSPLEKGAPLFPRKETE"
     gene            1330..2085
                     /locus_tag="VFX21_17475"
     CDS             1330..2085
                     /locus_tag="VFX21_17475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX5097817.1"
                     /translation="MWVDSHCHAHSAADADAQIERAHDAGVDWMVCVGTDLPTSHAAL
                     ELAARHHHVYGTVGLHPHDASKMAAEWGMLEALAATEECAGIGEAGFDLYYEHSPRDE
                     QEVAFRAQIRLAKRLAKPLIIHSRDAWDDTFRVLEDEGVPDRTVFHCFTGGPVEARRA
                     LDLGTSLSFSGIVSFKAAEDLRDAARLAPPDRILVETDAPYLTPEPFRGKPNEPALVT
                     YVGAALARARGEHEDVVAANTRANAARIFGVAE"
BASE COUNT          330 a          749 c          695 g          315 t
ORIGIN      
        1 gaagcagctc gtcgacgaga ccgcggcggc cttccgcgag acctgggacc tgctcgacat
       61 cgcctacgac gacttcatcc gcacgaccga gccccgccac taccaggccg tgcagcagtt
      121 cctccaggtc ctctacgaca acggcgccat cgagctcggc acctacgagg gcctctactg
      181 cgtctcgtgc gaggcctatt acaccgagga cgagctgatc gacggcaact gcccgatcca
      241 cggccggccg gtcgagcacg tcaccgagga gaactacttc ttccgtctct cgcgctacga
      301 gcaacgcctc ctcgaccact acgccgcgca ccctgacgcg gtgcagcccg actaccgcat
      361 gaacgaagtg ctcgggttca tccgccaggg gctgcgcgac ttctcgatga gccgcacgtc
      421 gatctcgtgg ggcgtgccgc tcccgtggga cccgaagcac gtcgcctacg tgtgggccga
      481 cgcgctgttc aactactgca ccgcggtcgg ctacggcgcg gacgacgcgc gcttcgcgcg
      541 gttctggccg gtcgactacc acctcgtcgg caaggacatc ttgcgcttcc acgcggtcta
      601 ctggcccgcg atgttgatgg ccgccggtct cgagccgccc gactgtgtgt acgcgcacgg
      661 ctggttgctc gtcggcggcg agaagatgtc gaagacgcgg ctcaaccaga tcgcgcccgc
      721 cgatctcgtc gccgacttcg gtgtcgacgg cttccgctat cacttcatgg ccgaccagcg
      781 cttcggcccc gatggcgact tctcgtacga ggcgatggtg ctgcgttaca acgcggacct
      841 cgccaacaac ttcggcaacc tcgcgaaccg cgtgctgaac atggcggtga actacgtcgg
      901 cggcgtggtg cccgacgggc gcgccgacgg tccgttgatg ggcgcggcga cggaagcgtt
      961 cgacctcgtg cagggcgcga tggcgaagct cgacttcgcc ggcgcgctcg cggccgtctg
     1021 ggacttgatc cgcgccgcga actcatacat cgaagaacgc cagccgtggg cgttgcacaa
     1081 ggccggcaac gcgaccgagg tcgcggaagt gctgggcgac tgcctggagg cgttgcgcat
     1141 cgtgacgttg ctggcgtcgc cggtgatccc gaacgcgacc gcggagctgt ggcggcgact
     1201 cggcctcgcg ggccggccgg aggacacgcg cctgcccgac gccgcgaagt ggggacagct
     1261 cgcgccggga tcgccgctcg agaagggcgc accgctcttc ccgcgcaaag agaccgagta
     1321 gctcgtttcg tgtgggtcga ttcccattgc cacgcgcact cggccgccga cgccgacgcg
     1381 cagatcgagc gcgcgcacga cgccggtgtc gactggatgg tctgcgtcgg caccgacctg
     1441 ccgacctcgc acgccgcgct ggagctcgcg gcgcgccacc accacgtgta cggcacggtc
     1501 gggctgcatc cgcacgacgc gtcgaagatg gccgcggaat gggggatgct cgaagcgctc
     1561 gccgcgaccg aggaatgcgc cggcatcggt gaggccggct tcgacctcta ctacgagcac
     1621 tcgccgcgcg acgagcagga agtcgcgttc cgggcccaga tccggttggc caagcggctc
     1681 gcgaagcctt tgatcatcca ctcgcgcgac gcgtgggacg acacgttccg cgtcctcgaa
     1741 gacgaaggcg tgcccgatcg caccgtgttc cactgcttca ccggagggcc ggtcgaagcg
     1801 cggcgcgcgc tcgacctcgg cacgtcgctg tcgttcagcg gcatcgtgtc gttcaaagct
     1861 gccgaagacc tgcgcgacgc ggcgcggctg gcacctcccg accgcatcct cgtcgagacc
     1921 gatgcgccgt acctcacccc cgagccgttc cgcgggaagc cgaacgagcc cgcgctggtc
     1981 acctacgtcg gcgccgcgct ggcacgcgcc cgcggcgagc acgaagacgt cgtcgccgcg
     2041 aacacgcgag ccaacgcggc gcgcatcttc ggcgtggcgg agtgacacc
//
LOCUS       DASUGA010000885         2279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1225195, whole genome shotgun sequence.
ACCESSION   DASUGA010000885 DASUGA010000000
VERSION     DASUGA010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225195"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2116
                     /locus_tag="VFX21_17480"
     CDS             <1..2116
                     /locus_tag="VFX21_17480"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gluconolaconase"
                     /protein_id="HEX5097818.1"
                     /translation="SRRAARAVLVLVLAVLTVVVVFGYFYGKAILPDPRPPLEAGWTA
                     VVEVLAGDGHLGSDDGPATRARFSDLFGVAVAPDGTVFIADAGASQRIRRVTIAGEVS
                     TVAGSVAGYADGTGESARFDTPSGIALGPDGAIYVADTGNNAIRRVTAAGAVSTIAGG
                     RAAGYRDGIVLDAEFNGPIGVAVEPSGRIIVADTYNDRIRAIERGGSVVTIAGSGRQG
                     HQDGPALDAQFDTPCGIAVDGAGNIFVADAGNGAIRKIDTSGMVSTVELSPPAAFLLR
                     PLGVAVADDGVIYATEDPGRVVEIVPGRSMRVIAGSLAGFANGRGDVARMRGAAAVAI
                     AGRGRLVVTDPRNSLIRLVTAISQADARPPSPPTLQPAFDEEQFASQPLLWPADPMLG
                     PHEITGTMGEARGAENSGRFHAGLDVQAFEGSPVRVVRNGTVTNPVATSDFGTLNEAF
                     RVGSVAYVHLRAGRTRRNDPLDDPRFVISRDENGHVNHVRVKRGSRFTTGEQIGTVNA
                     FNHVHLNVGWPGEEINPLRLRLVQFADRIAPTIAAIHLVTLGGQRLEAERRQPLVVDQ
                     PVQVVVDAWDQSDGNRKTRRLGLYELGYQILQTDGTPVPGFETPRQTIRFDQLSPDPD
                     APSLVYASGSGIPFYGSRTTHFLYVVTNSLAGGRAARGVWDPSELQPGNYIIRVTARD
                     FSGNEAGSGRDLAVVVGNNREP"
BASE COUNT          366 a          765 c          771 g          377 t
ORIGIN      
        1 gtcacgacgc gcggcgcgag ccgttctggt tctcgtcctc gccgttctca ccgtcgtcgt
       61 cgtctttggc tacttctacg ggaaagcgat tcttcccgac ccccggccac cactggaggc
      121 cggctggacc gccgtcgtcg aggtgctcgc cggcgatgga cacctgggct ctgacgatgg
      181 tccggcgaca cgcgcgcgct tttcggacct gttcggggtt gctgtggcgc cggacggtac
      241 cgtgttcatc gccgacgcgg gggcgtcgca gcggatccgc cgagtgacga ttgcaggcga
      301 ggtgtcgacg gttgcgggga gcgttgccgg ttatgcggac ggcacaggcg aatcagccag
      361 gttcgatacg ccctccggca tcgcgctcgg acccgacgga gcgatctacg tcgccgacac
      421 cggcaacaac gcaatccgtc gcgtgaccgc cgccggcgcc gtgtccacga ttgcaggggg
      481 acgcgcggcc ggctaccgcg atggcattgt cctcgatgcg gaattcaacg ggccgatcgg
      541 cgtcgcggtc gagccctcgg gccggattat cgtggccgac acgtacaacg atcgcatccg
      601 cgcgatcgaa cgcggcgggt cggtcgtcac gatcgcagga tcgggacgac aggggcacca
      661 ggacgggccg gccctcgacg cacagttcga cacaccctgt gggatcgccg tcgacggggc
      721 cggcaacatc ttcgtggccg atgccgggaa cggcgccatc cggaagatcg acacgtcagg
      781 catggtgtcg accgtcgagc tgtcaccccc ggcagcgttt ctcctgcggc cgctcggcgt
      841 cgctgtcgcc gacgacggcg tcatctacgc gaccgaagat cccggtcgtg tcgtcgagat
      901 cgtgccgggg cggagcatgc gggtgatcgc tggctcgctc gcggggtttg ccaacgggcg
      961 tggcgacgtg gcccggatgc gtggcgctgc cgcggtggcg attgccggcc gcgggcgcct
     1021 cgtggtgacg gatccgcgca actcgctcat ccgtctggtg accgcgatca gccaggccga
     1081 tgcccgccca ccgtcacctc cgacgctgca gccggcgttc gacgaggagc agttcgcgag
     1141 tcagccactc ctctggccgg cggatccgat gctgggacct cacgagatca ccggcacgat
     1201 gggcgaagcg aggggcgccg aaaactccgg ccggttccac gccggtctcg acgtgcaggc
     1261 cttcgaagga tcgccggtcc gcgtcgtccg caacgggacg gtgacgaatc cggtggcgac
     1321 ctcggacttc ggaacgctca atgaggcgtt ccgcgtcggg tcggttgcgt acgtgcacct
     1381 gcgcgcgggc cgcaccaggc ggaacgaccc gctcgacgat ccacgcttcg tcatctcacg
     1441 ggacgagaac gggcacgtca atcacgttcg cgtgaagcgc ggctcgaggt tcaccaccgg
     1501 tgaacagatc ggcaccgtca acgcgttcaa ccacgtgcac ctgaacgtcg gctggcccgg
     1561 cgaagagatc aatccgctgc gcctgcggct ggtgcagttc gccgatcgga ttgccccgac
     1621 catcgcggcg atacacctgg ttacgctcgg cgggcagcgg ctcgaggcgg aacgacgcca
     1681 gccgctcgtc gtcgatcagc ccgtgcaggt cgtcgtcgac gcgtgggatc aatccgacgg
     1741 caacaggaag acgaggcgcc tcggcctcta cgagctcggg tatcagatcc tgcagacgga
     1801 cggtacgccc gttccaggtt tcgagacacc ccgccagaca atccggttcg accaactctc
     1861 accggatccc gacgcgccgt cactcgtgta cgcgtccggc agcggcatcc cgttctatgg
     1921 cagtcggacg acgcacttcc tctacgttgt gacgaattcg ctcgcgggcg ggcgagccgc
     1981 gaggggtgtg tgggatccgt ccgaactgca accgggcaat tacatcatcc gcgtcaccgc
     2041 gcgcgacttc agcggcaacg aagcgggctc cggccgggac ctggcggttg tggtgggaaa
     2101 caatcgtgag ccgtgaatgg cgaattggcg aatggtgaaa acccgaaccg cctccattca
     2161 cgattcatgc ttcacgattc gccctgatca gatcagtccg ccgtcggcat ctcggcgctg
     2221 tcgatgacga gcaccggcac gggtgccttc tgcgtatcca gcttcaggcc gagccgctc
//
LOCUS       DASUGA010000886         2040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1535843, whole genome shotgun sequence.
ACCESSION   DASUGA010000886 DASUGA010000000
VERSION     DASUGA010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535843"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VFX21_17485"
     CDS             <1..360
                     /locus_tag="VFX21_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5097819.1"
                     /translation="PATREKWRAYTSLIEPDTREAFLQTLRSVVDVKGQRVSAHDKLY
                     LAEAIPSLIVWGENDPIIPVSHAYDAHAAMPASRLEIMEGVGHFPHCEDPRQFARILL
                     DFIANTDAAQRFEAAPA"
     gene            373..918
                     /locus_tag="VFX21_17490"
     CDS             373..918
                     /locus_tag="VFX21_17490"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5097820.1"
                     /translation="MADRFASISPELAEWWAAQPVFFVATAPAGTDGHVNLSPKGHDT
                     LRILGPNRVAYLDLTGSGVETIAHLRENGRITLMACAFNGNPQISRIYGTGTVHPLGA
                     PGFDALAAEFPDLPGRRSVIEVEVERVSTSCGYAVPLMDLVDERDRLIEWAKKKGDDG
                     IVKYWGNKNAHSIDGLPGLPA"
     gene            915..2006
                     /locus_tag="VFX21_17495"
     CDS             915..2006
                     /locus_tag="VFX21_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX5097821.1"
                     /translation="MNYAERIQRVRTRMDELGVDVTLLSVGPDLPYLTGYEAMPLERL
                     TMLVLPRAGDAALVVPRLEAPRVDAQPELFEIVPWEETDDPIEQVARRIGDGPRQIAM
                     GDHTWARFVLDLQHALPQSSFVRATEVVGPIRMVKDPAEIDALTKAAHAVDEIAGDMR
                     YAQFAGRTELDVHREFVDRIIAAGHERANFAIVAASENGASPHHEPSPDRIIADGDLV
                     LCDFGGTMKGYCSDITRMYSVGEPANEILDVYAVLVEAQEAGVQAATVGTPCEAVDAA
                     ARAVIAQAGLGDLFIHRVGHGIGTEAHEDPYMVAGNTRLLEVGHAFSVEPGIYFPGRV
                     GMRLEDIVVATEYGPVRLNRAPRDIAVVA"
BASE COUNT          346 a          670 c          691 g          333 t
ORIGIN      
        1 cccgcgacgc gggagaagtg gcgcgcgtac acctcgttga tcgagcccga cacgcgcgaa
       61 gcgttcttgc agacgctgcg ctccgtcgtc gacgtcaagg gtcagcgcgt gagcgcacac
      121 gacaagctct acctggccga ggcgatcccg tcgctcatcg tgtggggcga gaacgatccg
      181 atcattcctg tctcgcatgc gtacgacgcg catgccgcga tgccggcgag ccggctggag
      241 atcatggaag gcgtcggcca cttcccgcac tgcgaagacc cccggcagtt cgcccggatc
      301 ctcctcgatt tcatcgcgaa caccgacgcc gcgcaacgat tcgaggcggc tccggcctga
      361 cagaatgggg gcgtggccga ccgctttgcg tcgatcagtc ccgagctcgc cgagtggtgg
      421 gccgcgcaac cggtgttttt cgtcgccacc gcgccggccg gcaccgacgg gcacgtgaat
      481 ctttcgccca agggccacga cacgttgcgc atcctcggcc cgaaccgcgt tgcctatctc
      541 gatctcaccg gcagcggagt cgagacgatc gcgcacctgc gcgagaacgg acgcatcacg
      601 ctcatggcgt gcgcgttcaa cggcaacccg caaatctcgc ggatctacgg cacgggtacg
      661 gtgcatccgc tcggcgcgcc gggcttcgac gcactggccg ccgagtttcc cgacctgccg
      721 ggccggcggt cggtcatcga ggtcgaggtc gagcgcgtca gcacctcgtg cgggtacgca
      781 gtcccgttga tggatctcgt cgacgagcgc gaccggctga tcgagtgggc gaaaaagaag
      841 ggcgacgacg gcatcgtgaa gtactggggg aacaagaacg cgcacagcat cgacggactg
      901 ccggggctgc ccgcatgaac tacgcggagc gcatccagcg ggttcggacc cgcatggacg
      961 agctcggcgt cgacgtcacg ttgctctcgg tcggtcccga cctgccgtac ctcaccggtt
     1021 acgaagcgat gccgctcgaa cggctcacga tgctcgtgct gccgcgtgcg ggcgacgctg
     1081 cgctcgtcgt gccgcggctg gaggcgccgc gtgtcgacgc gcaacccgaa ctcttcgaga
     1141 tcgtcccttg ggaggaaact gatgatccga tcgagcaagt ggcgcgccgc attggcgatg
     1201 ggccccgtca gatcgcgatg ggcgatcaca cgtgggcgcg cttcgtgctc gatctgcagc
     1261 atgccttgcc gcagtcatct ttcgtacgtg cgacggaggt cgtaggaccg atccgcatgg
     1321 tgaaggaccc ggccgagatc gacgcgctga ccaaagccgc ccacgcggtc gacgagatcg
     1381 cgggcgacat gcgctacgcc cagtttgccg gtcgcaccga gctcgatgtg caccgggagt
     1441 tcgtggatcg catcattgcg gcgggacacg aacgagcgaa cttcgcgatc gttgccgcga
     1501 gcgaaaacgg cgccagcccg caccacgagc cgtcaccgga tcgcatcatc gccgacggcg
     1561 atctcgtgct ctgtgacttc ggcggcacga tgaagggcta ctgctccgac atcacgcgca
     1621 tgtattcggt cggcgaaccg gcgaacgaga ttctcgacgt gtatgcagtg ctcgtcgagg
     1681 cgcaagaggc gggcgtacag gccgcgacgg tcggtacacc gtgcgaagcg gtcgacgccg
     1741 ccgcccgggc cgtgatcgcg caggcagggc tcggcgacct tttcattcac cgcgtcggcc
     1801 acggcattgg gaccgaggcg cacgaagacc cctacatggt tgcgggcaat acccggttgc
     1861 tcgaggtcgg gcacgcgttc agcgtcgagc cgggcattta ttttccgggc cgggtcggga
     1921 tgcggctcga ggacatcgtg gtggcaaccg agtacgggcc ggtacggctc aaccgggcgc
     1981 cccgtgatat cgctgtcgtc gcgtgaaact cgatggcgcg acgctgctct tgcagttggc
//
LOCUS       DASUGA010000887         5784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_1364365, whole genome shotgun sequence.
ACCESSION   DASUGA010000887 DASUGA010000000
VERSION     DASUGA010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364365"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..671
                     /locus_tag="VFX21_17500"
     CDS             60..671
                     /locus_tag="VFX21_17500"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5097822.1"
                     /translation="MSVELEPTDRVKLRRKAERGSYERATVNAILDEALIAHVGFAID
                     GQTCVLPMTYARVDDVLYLHGAVANNMLKSLASGIDVCVTVTLLDAIVLSRSAFHHSM
                     NFRSVVVYGVATKVDSDDEKHVATRALVEHMVPGRSNETRGPTPSELRKTLFVRVPID
                     EASAKIRTGPPIEDPDDLALPYWGGEIPITLTRGEPVADEFVR"
     gene            complement(668..2188)
                     /locus_tag="VFX21_17505"
     CDS             complement(668..2188)
                     /locus_tag="VFX21_17505"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5097823.1"
                     /translation="MNVLLITTDQQRADTLGCYGSPLGATPRLDEFARQGTRFAAART
                     QNPLCQPARATILTGTYPSTHGVTCNGIDFGDDNIARTVPTLLGARGYRSAIVGKGHF
                     ATTYPYFPTGRIESVDGSALAPADWNGPYVGFDLAELIIFGHNLRIADLVGHWTWTYG
                     PPPFGLNYARYLVRDGHERGVERLRAMQPEAAGAQFDKRQTWRNQLAEEDHPTTWVAD
                     RACEWLRNVDGPFFGWVSFTDPHHPMDAPAPWCDRYAPADVLEIVPAVHEKEFENKPP
                     LHQALTHGVRDKAMAWANPGGANLSREDLAVMTAGYYGMVAQLDHNIGRVLDALTERG
                     VADDTLVIITTDHGEFMGEHQMIFKGPFGYDSLLRVPLLMRGPSVPAGHVVDEPVGTI
                     DIAPTALQAAGVEVPAWMEGRPLLDGPREHCLSENDFNIIGWIPMRTITTSRYKLHAY
                     LEHPWGELYDLREDPGELVNRFDDPGYAPIRADLEALLKETMNHNPRRDPTVGLVA"
     gene            complement(2185..2748)
                     /locus_tag="VFX21_17510"
     CDS             complement(2185..2748)
                     /locus_tag="VFX21_17510"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5097824.1"
                     /translation="MQPATDGRVQRGERNRAAIVDALLALLEDGVRRPSARQIAERAE
                     VSLRSVFQHFDDMESLYAECVRRQHERVTPLLTPIDVDAPLEERIEAVVVQRSRVFER
                     IAPIRRAAVQAAAESPVLRNGLAATAKRFREQTAAVFAAEVDDELLSVVDLVTSFDAW
                     EQLRTTQKLSVTNARRVMVRALKELMR"
     gene            2819..3724
                     /locus_tag="VFX21_17515"
     CDS             2819..3724
                     /locus_tag="VFX21_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HEX5097825.1"
                     /translation="MEALRTPDSRFEHLTGFPFAPHYVDVASGEGSTLRVHYLDEGPR
                     DAPIVLLMHGEPSWCFLYRTMIPVLVGAGLRCVAPDLVGFGRSDKPTKRTDYTYERHV
                     AWMREALFDTLDLRDITLMCQDWGGLIGLRLVGEHPDRFARVVAANTFLPTGDNPPGE
                     AFLWWQNFSQTVEDFAVGGIVNGGCTTDLTPEVIAGYDAPFPDDSYKEGARQFPLLVP
                     TSPDDPASAANRTAWEVLKKFEKPFLCAFSDKDPITGGGDRVLERDIPGCKGQPHTTI
                     EGGGHFLQEDRGPELARVIAAFVQG"
     gene            3881..4327
                     /locus_tag="VFX21_17520"
     CDS             3881..4327
                     /locus_tag="VFX21_17520"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5097826.1"
                     /translation="MRARFENDLGYVSAKVGANAAIFDDDDRILLAHRADDGCFGLLA
                     GWIDPNEDPYETIVREIAEEAGVTGRVDKLVGVFGRGGHSGDGPHGAVAIVFLCSLDS
                     HNFVAQEHEVVNLAFHRIEDVTNWHKDHETYARAAREAWWRHRAGL"
     gene            4300..4809
                     /locus_tag="VFX21_17525"
     CDS             4300..4809
                     /locus_tag="VFX21_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5097827.1"
                     /translation="MVAASGRPLSEWVCLALVAEAPTHGWAVAQLLASEGEIGRVWSL
                     SRPLTYRALDQLIADTVVSRSGVEHARGPARTILTATPNGKRAARKWLRTPIEHLRDV
                     RTELLCKVLLSQRAGIDPRALLEAQKVEFAPRFEALARRARAKDADVVDRWRHATADA
                     ARRFLEGET"
     gene            4845..5633
                     /gene="modA"
                     /locus_tag="VFX21_17530"
     CDS             4845..5633
                     /gene="modA"
                     /locus_tag="VFX21_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5097828.1"
                     /translation="MRKLLAVLTLSALALAFTACGGDDDDNDTAAGDTTSGPTGSITV
                     FGAASLTEAFTTLGEDFEAEHPDTKVEFNFAASSELVTQIQNAAPADVFASADASNMT
                     KLTDASLTAGQPEVFAHNQLAIAVEPGNPKGITALADLSNPDITLVLCAPEVPCGKYA
                     DQALQQAGVTVNPVSREASVKATLTKVELGEADAAIVYVTDVTSSGKVEGIDIPDDQN
                     VVATLPITTLKDAGNADLAAEWVDYVLSPAAQKVLQDDYGFLAP"
BASE COUNT          932 a         1989 c         1929 g          934 t
ORIGIN      
        1 agaacgtggt cgcgaaattc gggcgctcgt agccgcgcgc gccactaccc tccccgggcg
       61 tgagcgtcga actcgaaccc accgatcgcg tcaagctccg ccgtaaggcc gagcgcggga
      121 gctacgagcg agcaacggtg aatgcgatcc tcgacgaagc gctcatcgcg cacgttggct
      181 tcgcgatcga cgggcaaaca tgcgtgctgc cgatgaccta cgcccgcgtc gacgacgtgc
      241 tgtacctgca cggcgcggtc gccaacaaca tgttgaagtc gttggcatcc ggcatcgacg
      301 tctgcgtgac cgtcaccctg ctcgacgcaa tcgtgctgtc gcgatcggca ttccaccact
      361 cgatgaactt ccgttccgtc gtcgtgtacg gcgtcgccac gaaggtcgac tccgacgacg
      421 agaagcacgt cgcgacgcgc gcgctggtcg agcacatggt gccggggcgc agcaacgaga
      481 cccgcggtcc gaccccgagt gaactgcgca agacgctgtt cgtgcgcgtg ccgatcgacg
      541 aggcatcggc caagatccgc accggcccgc ccatcgagga ccccgacgac ctcgcgctcc
      601 cctactgggg cggtgagatc ccgatcaccc tcacccgcgg cgaacccgtc gccgacgagt
      661 tcgtccgcta ggccacgagg ccgacggtgg gatcgcggcg ggggttgtgg ttcatcgttt
      721 ccttcagcag cgcctcgagg tcggcgcgga tcggcgcgta gccggggtcg tcgaagcggt
      781 tgacgagctc gccgggatct tcccgcaagt cgtagagctc gccccatggg tgctccaagt
      841 acgcgtgcaa cttgtagcgg ctcgtcgtga tcgtgcgcat cgggatccac ccgatgatgt
      901 tgaagtcgtt ctcgctgagg cagtgctcac gcgggccgtc gagcagcggg cgcccttcca
      961 tccacgccgg cacctcgacg ccggctgctt gcagcgcggt cggcgcgatg tcgatcgtcc
     1021 ccacgggttc gtcgacgacg tgaccggccg gcaccgaggg ccctcgcatc aacagcggca
     1081 cgcgcagcaa gctgtcgtag ccgaatgggc ccttgaagat catctggtgc tcgcccatga
     1141 actcgccgtg gtcggtggtg atgatgacga gcgtgtcgtc cgcgacgccg cgttcggtga
     1201 gcgcgtcgag cacgcggccg atgttgtggt cgagctgggc gaccatgccg tagtagcccg
     1261 cggtcatcac cgcgaggtct tcgcgcgaga ggttggcgcc gccggggttc gcccatgcca
     1321 tcgccttgtc gcgtacaccg tgcgtcaggg cctggtgcag cggcggcttg ttctcgaact
     1381 ccttttcgtg gaccgcggga acgatctcca acacgtcggc cggtgcgtaa cggtcgcacc
     1441 acggcgccgg cgcgtccatc gggtggtgcg gatcggtgaa cgagacccag ccgaagaacg
     1501 ggccgtcgac gttgcgcaac cactcacacg cgcggtcggc gacccacgtc gtcggatggt
     1561 cttcttcggc cagttggttg cgccaggtct ggcgcttgtc gaactgtgcg cccgccgctt
     1621 cgggttgcat cgcgcgcagc cgctcgacgc cgcgctcgtg cccgtcgcgg accaggtagc
     1681 gcgcgtagtt gaggccgaag ggtggcggtc cgtatgtcca cgtccagtgg ccgacgaggt
     1741 cggcgatgcg caggttgtga ccgaagatga tcagttcggc gaggtcgaag ccgacgtacg
     1801 ggccgttcca gtcggcgggt gcgagtgcgg agccgtcgac cgactcgatg cgacccgtcg
     1861 ggaagtacgg atacgtcgtc gcgaagtggc ccttgccgac gatcgcgctc cgatagccgc
     1921 gcgcgccgag caacgtcggc acggtgcggg cgatgttgtc atcgccgaag tcgataccgt
     1981 tgcacgtgac gccatgcgtc gacgggtacg tccccgtgag gatggtcgca cgcgccggct
     2041 ggcacagcgg gttctgcgtg cgggccgcgg cgaaccgggt gccctggcgg gcgaactcgt
     2101 cgaggcgggg cgtggcgccg agggggctcc cgtagcagcc aagcgtgtcg gcgcgctgtt
     2161 ggtcggtggt gatcaacaag acgttcatcg catgagctcc ttcaacgcgc gcaccatcac
     2221 gcggcgcgca ttcgtgaccg acaacttctg ggtcgtgcgc agctgctccc acgcgtcgaa
     2281 cgaggtgacg aggtcgacca ccgacagcaa ttcgtcgtcg acctcggccg cgaacactgc
     2341 ggcggtctgc tcgcggaacc gcttggcggt ggccgcgagc ccgttccgca ggaccggcga
     2401 ctcggcggcc gcctggacgg ccgcccgccg gatcggcgcg atgcgctcga acacgcggct
     2461 gcgttgcacg acgacggctt cgatccgttc ctcgagtggc gcgtcgacat cgatcggcgt
     2521 gaggagcggc gtgacgcgtt cgtgttgccg gcgaacgcat tccgcgtaga gcgactccat
     2581 gtcgtcgaag tgctggaaca cggaccgcag cgacacttcc gcgcgctcgg cgatctgccg
     2641 ggccgacggc cgccggacgc cgtcttcgag cagggcgagc aacgcgtcga cgatcgcggc
     2701 gcggttgcgc tccccgcgct gcacgcgacc gtcggttgcc ggttgcacgg aaccatggta
     2761 gaactactcg cactttcagt gcaactatct gccccggcgc cgatccagga gggtcctcgt
     2821 ggaggccttg cgcacgcccg acagccggtt cgagcacctc accggtttcc ccttcgcacc
     2881 ccactacgtc gacgtcgcgt cgggtgaggg ctcgacgttg cgggtgcact acctcgacga
     2941 aggcccgcgc gacgcgccga tcgtgttgct gatgcacggc gagccgtcgt ggtgcttcct
     3001 ctaccgcacc atgatcccgg tactcgtcgg cgccggcctg cgttgcgtcg cgcccgacct
     3061 cgtcggcttc ggtcgctccg acaagccgac caagcgcacc gactatacgt acgaacgcca
     3121 cgttgcgtgg atgcgcgaag cgctcttcga cacgctcgac ctgcgcgaca tcacgttgat
     3181 gtgccaggac tggggcggct tgatcggact gcggctcgtc ggcgagcacc ccgatcgctt
     3241 cgcacgcgtc gtcgccgcga acacgttcct tcctaccggc gacaacccgc ccggcgaagc
     3301 gttcttgtgg tggcagaact tctcgcagac cgtcgaggac ttcgcggtcg gcggcatcgt
     3361 gaacggcggg tgcacgactg atctcactcc cgaggtgatc gccgggtacg acgcgccctt
     3421 ccccgacgac tcctacaagg agggagcgcg gcaattcccg ttgctcgtcc ccaccagccc
     3481 cgacgatccc gcgtcggccg cgaaccgcac cgcatgggaa gtgctgaaga agttcgagaa
     3541 gcccttcctc tgcgcgttct ccgacaagga cccgatcacc ggcggcggcg accgggtgct
     3601 cgagcgcgac attcccgggt gcaagggaca accgcacacg accatcgaag gcggcggcca
     3661 cttcttgcag gaagaccgcg gcccggagtt ggcgcgcgtc atcgcggcgt tcgtgcaagg
     3721 ctgacacatc gacgtcttcg gcctgctcga ccaaatccgc gcgatcgcgc aaacaggtct
     3781 gcacttcgcg accgacccgt tcgaccgcga gcgttacgag aagctgctcg atctcgcgtc
     3841 ccgcgagtac gcggaccgtc tctcactgga acccgaggca gtgcgcgcgc ggttcgagaa
     3901 cgacctcggc tacgtcagcg cgaaggtcgg cgccaacgcc gcgatcttcg acgacgacga
     3961 ccgcatcctc ctcgcccacc gcgccgacga cggatgtttc ggcctgttgg ccggctggat
     4021 cgacccgaac gaggacccct acgaaacgat cgtgcgtgag atcgcggagg aagccggcgt
     4081 cacggggcgg gtcgacaagc tcgtcggcgt cttcggtcga ggtgggcact cgggagacgg
     4141 gccccatggc gcggtcgcga tcgtcttcct ctgctctctc gacagtcaca acttcgtcgc
     4201 gcaggaacac gaggtggtca acctcgcgtt ccaccgcatc gaggacgtca ccaactggca
     4261 caaggaccac gagacgtacg cgcgcgcggc gcgggaggca tggtggcggc atcgggccgg
     4321 cctctgagcg agtgggtgtg tctcgccctc gtcgcggaag cgccgaccca cggctgggcg
     4381 gtcgcacaac tcctggcatc cgagggtgag atcggacgcg tctggtcgct gtcgcgcccc
     4441 ttgacgtatc gcgcgctcga ccaactcatt gccgacaccg tggtgtcgcg tagtggcgtc
     4501 gagcacgcac gcggaccggc gcgcacgatc ctgaccgcga cgccgaacgg caagcgcgcg
     4561 gcgcgcaagt ggttgcgcac gccgatcgag catctgcgag atgtccgtac tgagctgctg
     4621 tgcaaggtgc tgctcagtca acgcgcgggc atagatccgc gcgcgctact cgaagcacag
     4681 aaggtcgagt tcgccccgcg cttcgaggca ctggcgcggc gcgccagagc caaggacgcg
     4741 gacgtcgtcg accggtggcg gcacgccacg gccgatgcgg cccgccgctt cctcgagggc
     4801 gagacttgac gtcactagtc aactcctgac tattgtcccg ctccatgcgc aaattgctcg
     4861 ccgtcctgac cctgtccgcg ctcgcgctcg cgttcaccgc gtgcggcggt gacgacgacg
     4921 acaacgacac ggccgccggc gacacgactt ccggaccgac cggttcgatc acggtgttcg
     4981 gcgccgcgtc gctgaccgag gcgttcacca cgctcggcga agacttcgag gccgagcacc
     5041 ccgacacgaa ggtcgagttc aacttcgcgg cgtcgtcgga gctcgtcacc cagatccaga
     5101 acgcggcacc cgccgatgtg ttcgcgtcgg ccgacgcgtc gaacatgacg aagctcaccg
     5161 acgcgtcact gaccgccggt caacccgagg tctttgcgca caaccagctc gcaatcgcgg
     5221 tggagcccgg caacccgaag ggcatcaccg cccttgccga cctgagcaac cctgacatca
     5281 ccctggtgct gtgcgcgccg gaagtaccgt gcggcaagta tgccgaccag gccctccagc
     5341 aagcgggtgt caccgtgaac ccggtgtcgc gcgaagcgtc ggtcaaggcg acgctgacga
     5401 aggtcgagct cggtgaagcc gacgccgcca tcgtctacgt gaccgacgtg acgtcgagcg
     5461 gcaaggtcga aggcatcgac atccccgacg accagaacgt cgtcgcgacg ctgcccatca
     5521 ccacactgaa ggacgcgggg aacgcagacc tggccgcgga gtgggtcgac tacgtcctct
     5581 cccccgccgc acagaaagtg ctgcaggacg actacggctt cctcgcgccg tgacgcaacc
     5641 atcgcggcga gcacgagttc cggccttcgc cgtcgggctc gccgtgatcg gcctgctcgt
     5701 cttcttcgcg gcaccgttgg tcgcactggt ccagcgcgtg ccgtggagcc agttcttcga
     5761 cgacctcagt gatcctgcgg tccg
//
LOCUS       DASUGA010000888         5224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_320677, whole genome shotgun sequence.
ACCESSION   DASUGA010000888 DASUGA010000000
VERSION     DASUGA010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320677"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /gene="msrA"
                     /locus_tag="VFX21_17535"
     CDS             <1..535
                     /gene="msrA"
                     /locus_tag="VFX21_17535"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015132642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX5097829.1"
                     /translation="DAPLEGPFPDGYEMAMFGMGCFWGAERKFWQLPGVYTTAVGYAA
                     GYTPNPTYEEVCSGRTGHTEAVLVVFDPEQISYDDLLRTFWENHDPTQGMRQGNDIGT
                     QYRSGIYVYSDAQRDAAKKSRDMFQARLGDKGYGEITTEILDAPEFYYAEDYHQQYLA
                     KNPNGYCGLGGTGVTCT"
     gene            662..1615
                     /gene="coaA"
                     /locus_tag="VFX21_17540"
     CDS             662..1615
                     /gene="coaA"
                     /locus_tag="VFX21_17540"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEX5097830.1"
                     /translation="MDDDTSPEFSPYVDFTRAEWSRLRAATPLSLTEADLGALRGRNE
                     PVALAEVEEVYLPLSRLLNLRFAATRDLYAATETFLGQLPGRVPYVIGIAGSVAVGKS
                     TISRILQALLMRWPDHPRVDLVTTDGFLHPNAVLESRGLMSRKGFPESYDVRNLLRFL
                     AELKAGEPVVRAPVYSHITYDIVPGEEIELRSPDIVIVEGLNVLQAPARRGAAEPTVV
                     VSDYFNFTIYVDAEAAHVEQWYLDRLVLLRETALRDPESFFHFLTDYTEEDTRAFGQH
                     IWREVNAVNLRENIEPTRGRAHLILEKGADHTVERVRLRKL"
     gene            complement(1627..2235)
                     /locus_tag="VFX21_17545"
     CDS             complement(1627..2235)
                     /locus_tag="VFX21_17545"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5097831.1"
                     /translation="MRLPADQRRQQLLDVARDVFAATGYHETSMDDIAEGAGVTKPVL
                     YQHFPSKRALYLELLTDTGRQLLDALSSATERVPTGRERVEAGFRAYFQYVTRNRSGF
                     RLLFGASIRTDSEFAAVVDGVVRSAADAISELIEIPASDEHRRVLATALVGMAESVGR
                     RASDGVDEQHEVTDAAELAHWIAELAWFGLRGVRSEEGATLQ"
     gene            complement(2251..3258)
                     /locus_tag="VFX21_17550"
     CDS             complement(2251..3258)
                     /locus_tag="VFX21_17550"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5097832.1"
                     /translation="MRFRRLGNSDLVVSEVGLGTWTLVSDWWGKSEDPAAIIRAALDA
                     GINFIDTAPVYGQDGMGETILREVLQSNTDIVLTTKVGYDITAERKFPGQSERPHDWR
                     PESVRAQCDDSLARLGVECIDLYQLHNTRIEPIQADDLWETLLDLKREGKIRELGVAL
                     GPAIGWVEEGNQAIDNRPIATLQTVFNILEQEPGLTFARRPRVQSGEIGLLARVPHAS
                     DTLSGKVTPDTVFDPKDHRSHRNRDNMLDNFEKAATLSFLWAPETGRTMGQAAVAGIL
                     ANPTFANVLPTVLTVADIVEYAAASDLPLTADEARQVDELFARNFDHEDRYTMPLKSS
                     V"
     gene            complement(3283..4392)
                     /locus_tag="VFX21_17555"
     CDS             complement(3283..4392)
                     /locus_tag="VFX21_17555"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEX5097833.1"
                     /translation="MPKAAVCLGINEPMEVVDVNLDAPKAGEVRVRMGASGVCHSDLS
                     VVNGTLLSPLPSVLGHEGAGIVDAVGDGVSSVKLGDHVVLSFVPQCGKCYFCTHDTPE
                     MCEPGFMAMATGGQLDMTSRFSRTDGPLHQMSGLGTFSEELVCPEISTVKVDPDVPLT
                     SAALIGCGVLTGFGAAANTADVRTGDTVAVIGCGGVGLNVIQGAKFKGAERIIAVDQF
                     DSKLQMAKDFGATDLVNAKNGDAVAQVQELTNGRGADVAFEVIGLKVTVQQALAMARR
                     GGQAIIVGVPKMEQIMEIPIAMELLVNEKQVRGSWYGSSAVQRDVPQLVGLYQKGELK
                     LDELVSRKIGLDGINDAFKAMEAGEVARSVIDYGI"
     gene            complement(4603..4845)
                     /locus_tag="VFX21_17560"
     CDS             complement(4603..4845)
                     /locus_tag="VFX21_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097834.1"
                     /translation="MAERRAKIARIAAIGTRLGYLALAVSIVAFFVGLATDWPNWTVT
                     LSIAGLVFACIVMPLPIVLGYGVRAAEREDRERGNA"
     gene            complement(4854..>5224)
                     /locus_tag="VFX21_17565"
     CDS             complement(4854..>5224)
                     /locus_tag="VFX21_17565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5097835.1"
                     /translation="ARAATPMTVGRAVTAAGAYAACDVIVFPSTWEGFGNPVIESIAA
                     RRPLAAFAYPVLAEILASGVRVFSTDTPEDVVRFLAQPEAQRETYYDVNLHRARLSFA
                     ITELPRAIDEAFANHGWVSW"
BASE COUNT          820 a         1775 c         1787 g          842 t
ORIGIN      
        1 cgacgcgccg ctcgagggac cgttccctga cggctatgag atggcgatgt tcgggatggg
       61 gtgcttctgg ggcgcagagc ggaagttctg gcagctcccc ggcgtctaca cgaccgcggt
      121 cggttacgcc gcgggctaca cgccgaaccc gacctacgaa gaggtgtgct cgggccgcac
      181 cggccacacg gaggcggtat tggtcgtctt cgatcccgag cagatctcct acgacgacct
      241 gctgcgcacg ttctgggaga accacgaccc gacgcaaggc atgcggcagg gcaacgacat
      301 cggcacgcag taccgctccg gcatctacgt gtacagcgac gcgcagcgcg acgcggccaa
      361 gaagtcgcgt gacatgttcc aggcgcggct cggcgacaag ggctacggcg agatcacgac
      421 ggagatcctc gacgcgcccg agttctatta cgccgaggac taccaccagc agtacttggc
      481 caagaacccc aacggctact gcggcctcgg tggcaccggc gtcacctgca catagccctt
      541 tgtcgctccg cacgtgctcg cgtgaggcgg cccggattgt tggcccgcgc tccgctccgc
      601 gagtgcctcc gcaagctccg gcaccgcctc gtgtgacctg aaccggtagc gtcgcgccct
      661 tgtggatgac gacacgtcgc cggagttctc gccgtacgtc gacttcactc gggccgagtg
      721 gagccggctg cgcgcggcga cgccgctgtc gctgaccgaa gccgatctcg gcgccttgcg
      781 cggccgcaac gaaccggtcg cgctcgcaga agtcgaagag gtgtacctcc cgctgtcgcg
      841 gctgttgaac ctgcggttcg cggcgacgcg tgacctgtac gcggccacgg agaccttcct
      901 cggccaactc cccgggcgcg tcccgtacgt gatcggcatc gcgggcagcg tcgcggtcgg
      961 caagagcacg atctcgcgca tcctccaggc gttgttgatg cgatggcccg accacccgcg
     1021 cgtcgacctc gtgacgaccg acggcttcct gcacccgaac gcggtgctcg agtcgcgcgg
     1081 gttgatgagc cgcaaaggct tcccggagag ctacgacgtc cggaacctcc tgcgcttcct
     1141 cgccgagttg aaggccgggg agccggtcgt gcgggcgccg gtgtactcgc acatcaccta
     1201 cgacatcgtg ccgggcgagg agatcgagct ccgcagcccc gacatcgtga tcgtcgaagg
     1261 cctgaatgtg ctgcaggcgc cggcccgacg cggtgccgcg gagccgaccg tcgtggtgtc
     1321 ggactacttc aacttcacga tctatgtcga cgcggaggcc gcgcacgtcg agcagtggta
     1381 cctcgaccgg ttggtgttgc tgcgcgagac ggcgttgcgc gatccggagt cgttcttcca
     1441 cttcctcacc gactacaccg aagaggacac gcgcgcgttc ggccagcaca tctggcgcga
     1501 ggtcaatgcc gtgaacctgc gcgagaacat cgagccgacg cggggccggg cgcacttgat
     1561 cctcgagaaa ggcgcggacc acaccgtcga acgcgtccgc ctccgcaagc tgtagtcagg
     1621 agctctttac tgcagcgtcg cgccttcttc gctgcggacg ccgcggaggc cgaaccaggc
     1681 gagctccgcg atccagtgcg cgagctcggc ggcgtcggtg acctcgtgtt gctcgtcgac
     1741 gccgtcgctg gcgcgccggc ccaccgactc ggccattccg acgagtgccg tcgcgagcac
     1801 gcggcggtgc tcgtcggagg ccgggatctc gatcagctcg gagatcgcgt cggccgcgct
     1861 gcgtaccacg ccgtcgacga cggccgcgaa ctccgagtcg gtgcggatcg acgcgccgaa
     1921 caggagccgg aacccggaac ggttgcgcgt gacgtattgg aagtacgcgc gaaacccggc
     1981 ctcgacccgt tcgcggccgg tgggcacgcg ttcggtggcc gacgacagcg cgtcgagcag
     2041 ctgccggccc gtgtcggtca gcagctcgag atagagcgcc cgcttgctcg ggaagtgctg
     2101 gtacagcacc ggcttggtga cgcccgcgcc ttccgcgatg tcgtccatcg acgtctcgtg
     2161 gtaccccgtc gcggcgaaca cgtcgcgagc gacgtcgagc aactgctggc gccgctggtc
     2221 ggcggggagg cgcactttga gccggccggc tcagacgctc gacttgagcg gcatcgtgta
     2281 gcgatcctcg tggtcgaagt tgcgggcgaa cagctcgtcg acctggcgtg cctcgtcggc
     2341 ggtgagcggc aggtcggacg cggcggcgta ctcgacgatg tcggcgacgg tcagcaccgt
     2401 cggcagcacg ttcgcgaagg tgggattcgc gaggatgccg gcgaccgcgg cctgacccat
     2461 cgtgcggccg gtctccggcg cccacaggaa cgacaaggtc gcggccttct cgaagttgtc
     2521 gagcatgttg tcgcggttgc ggtgcgaccg gtggtccttc gggtcgaaca ccgtgtcggg
     2581 cgtcaccttg cccgagaggg tgtccgaggc gtgcgggacg cgggcgagca gaccgatctc
     2641 gcccgactgc acgcggggcc ggcgcgcgaa cgtgagcccg ggctcttgct ccaagatgtt
     2701 gaacacggtt tgcagtgtcg cgatcgggcg gttgtcgatc gcctggttgc cctcctcgac
     2761 ccagccgatc gcgggaccga gtgcgacccc gagctcgcgg atcttgccct cgcgcttgag
     2821 gtcgagcagc gtctcccaca ggtcgtcggc ctgaatcggc tcgatgcgcg tgttgtgcag
     2881 ctgatagagg tcgatgcact cgacgccgag acgcgccagc gaatcgtcgc actgcgcccg
     2941 caccgactcc ggccgccagt cgtgcggtcg ttccgattga ccggggaact tgcgctcggc
     3001 cgtgatgtcg tagccgacct tcgtcgtgag gacgatgtcg gtgttcgact gcaagacttc
     3061 gcgcaggatc gtctcaccca tgccgtcctg gccgtagacc ggcgcggtgt cgatgaagtt
     3121 gatcccggcg tcgagcgcgg cccgaatgat cgcggccgga tcctcgctct tgccccacca
     3181 atccgagacg agggtccagg tgccgagccc gacttccgag acgacgagat cagagtttcc
     3241 gagacggcga aatcgcatga cgcgaggcta cgcgacccgc ggtcagattc cgtagtcgat
     3301 gacggagcgc gcgacttcgc ccgcttccat cgccttgaac gcgtcgttga tcccgtcgag
     3361 cccgatcttg cgcgacacca gctcgtcgag cttcagctcg cccttctggt agaggcccac
     3421 gagctgcggc acgtcgcgct gcacggccga agagccgtac cacgagccgc gcacctgctt
     3481 ctcgttcacg agcagctcca tggcgatcgg gatctccatg atctgttcca tcttcgggac
     3541 accgacgatg atcgcctggc caccgcggcg cgccatggcg agtgcctgct gcaccgtcac
     3601 cttgagcccg atgacctcga acgcgacgtc ggcgccgcgc ccgttcgtca gctcctggac
     3661 ctgcgcgacc gcgtcgccgt tcttcgcgtt gacgaggtcg gtcgcgccga agtccttggc
     3721 catctgcagc ttcgagtcga actggtcgac cgcgatgatg cgctcggcgc ccttgaactt
     3781 cgcgccctgg atgacgttga gaccgacacc gccgcacccg atcaccgcga ccgtgtcgcc
     3841 cgtgcgcacg tccgcggtgt tggccgccgc gccgaagccg gtgagcacac cgcagccgat
     3901 gagcgccgcg ctcgtgagcg gcacgtcggg atcgaccttc acggtcgaga tctcggggca
     3961 gaccagctcc tcggagaacg tgccgaggcc cgacatctgg tggagcgggc cgtcggtgcg
     4021 gctgaagcgc gacgtcatgt cgagttgacc gccggtcgcc atcgccatga acccgggctc
     4081 gcacatctcg ggtgtgtcgt gcgtgcagaa gtagcacttg ccgcactgcg ggacgaacga
     4141 caagacgacg tggtcgccga gcttgacgga cgacacgccg tcgccgaccg cgtcgacgat
     4201 gcccgcgccc tcgtggccga gcacggacgg cagcggcgac agcagggtgc cgttcacgac
     4261 cgacaggtcg gagtggcata cgccggatgc ccccatgcgc acacggacct caccggcctt
     4321 gggtgcgtcg aggttgacgt cgacgacttc catcggctcg ttgatcccga gacagacggc
     4381 ggctttgggc atgtcgtggt ccttcctctg cgggtcgcgc gggtgcgtga cctgcgtcac
     4441 cttccgcggt gcgggttccc gcggcctacg ggccgcaact taccggaccg acaaattcgg
     4501 gcctgactcg gcgagtcctt cgcgctgaaa ccggcgggca tgttgagaag tcgcgagccg
     4561 acgaccgaac gcgtcacggg gaatgacgcg gtcgcaaccc ggtcaggcgt tgccgcgctc
     4621 gcggtcctcg cgttcggcgg cgcgtacgcc gtagccgagc acgatcggca gcggcatgac
     4681 gatgcacgcg aacaccaggc ccgcgatcga caaggtcacc gtccagttcg gccagtcggt
     4741 cgcgagcccg acgaagaagg cgacgatgct gaccgccagc gcgaggtacc cgaggcgcgt
     4801 cccgatcgcg gcgatgcgtg cgatcttcgc tcgccgttcg gccaccgggt cgttcaccat
     4861 gacacccagc cgtggttggc gaacgcctcg tcgatggcgc gcggcagctc ggtgatggcg
     4921 aaggagaggc gcgcccggtg gaggttgacg tcgtagtacg tctcgcgctg cgcctccggt
     4981 tgcgcgagga atcggacgac gtcttccggg gtgtccgtcg agaacacgcg cacgccggaa
     5041 gcaaggatct cggccagcac gggataagcg aatgctgcga gcgggcggcg cgcggcaatc
     5101 gattcgatga ccgggttgcc gaaaccctcc cacgtcgacg gaaagacgat cacgtcgcag
     5161 gccgcgtacg cgccggccgc ggtcacagcg cgaccgacgg tcatcggcgt cgccgcgcgc
     5221 gcgc
//
LOCUS       DASUGA010000889         3467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U1393
            k141_948856, whole genome shotgun sequence.
ACCESSION   DASUGA010000889 DASUGA010000000
VERSION     DASUGA010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961545
            Sequence Read Archive: SRR25158403
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:25:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,519
            CDSs (total)                      :: 3,497
            Genes (coding)                    :: 3,488
            CDSs (with protein)               :: 3,488
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 2 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948856"
                     /isolate="SMAG_U1393"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VFX21_17570"
     CDS             complement(<1..246)
                     /locus_tag="VFX21_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5097836.1"
                     /translation="MAETGWPPQAAVPDDLAHTLLREGEIDILGRMPWSSNATYLATV
                     TGDAQMLVVYKPQRGERPLWDFPRGTLCKREYAARVVS"
     gene            complement(256..774)
                     /locus_tag="VFX21_17575"
     CDS             complement(256..774)
                     /locus_tag="VFX21_17575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HEX5097837.1"
                     /translation="MADVIEFDPVDGIVAGAFGQPGARTFVIQARKGSALLSVLVEKE
                     QVALLATEAGQFLDRIDEDEPDDDDDDDVVSDPPIEDEPLFRARLIGIGYDPERHLVL
                     IELRETATEEEEAPPALEDSEGRIARLYATRAQVRAMVEVGAQAVSAGRPRCDLCDFP
                     MDPDGHICPRWN"
     gene            complement(781..1476)
                     /locus_tag="VFX21_17580"
     CDS             complement(781..1476)
                     /locus_tag="VFX21_17580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HEX5097838.1"
                     /translation="MTGAEAVDTAETNDEKNGAKEKPKPPTRLVLVRHAVTAHTGPLL
                     SGRMPGIDLSEKGRGQAAATADRLAKLPVAAVYASPIERTTQTAEEIAARHGLDVQPL
                     PGVIEADYGEWTGGKIADLAKTEEWKVVQVAPSRARFPGGETIRAMQARMVEALEEVV
                     AAHPHETFVVVSHADPIKSAIAHYTGMHLDLFQRVHVSPASVTVFDFHPFGSMMVKCN
                     DTGGLEDLLPTEE"
     gene            1521..1955
                     /locus_tag="VFX21_17585"
     CDS             1521..1955
                     /locus_tag="VFX21_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3151 family protein"
                     /protein_id="HEX5097839.1"
                     /translation="MTDVHLSASGPPETVLDPPDDDAADALRAALAQPIEGRRDAVAD
                     VCARWPRYIDAWARLGSLARDDVEAYACYRVGYHRGLDQLRASGWRGSGYVRWRHETN
                     RGFLRALDGLRAAAEAIGEADEADRCRMFLHQLDPDWDRRNE"
     gene            1967..2950
                     /gene="gmd"
                     /locus_tag="VFX21_17590"
     CDS             1967..2950
                     /gene="gmd"
                     /locus_tag="VFX21_17590"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX5097840.1"
                     /translation="MKRALITGITGQDGRHLSQLLATKGYQVFGLVHGQANPKIDLVQ
                     GENPSLELVEGDLRDLSSLIGVVEQVQPDEVYNLGAISFVQLSFKQAELTAEITGLGV
                     LRMLEAVRIVGGENNPIRFYQASSSEMFGKVRETPQTEETPFHPRSPYGAAKVFGHYT
                     TVNYREAYNIHASSGILFNHEGPRRGLEFVTRKVTNSVARIKLGLQDSISLGNLDSKR
                     DWGYAGDYVQAMWMMLQQDEPDDYVIATGETHSIRDFLDAAFELAEIDDWARYVKQDP
                     RFYRPTEVDLLIGDASKAKKKLGWEPSVSFPELVQMMYENDLKEESEKAAR"
     gene            complement(3027..>3467)
                     /locus_tag="VFX21_17595"
     CDS             complement(3027..>3467)
                     /locus_tag="VFX21_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015625238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein oxidoreductase"
                     /protein_id="HEX5097841.1"
                     /translation="HANKQGRVAGVNATGGHASFGGVVGTAVTRICNVEIGRTGLTEH
                     EAKDAGFDYVTAKIESTTRAGYYPGASKITVKVLAEKSSGRLLGAQIIGREGAAKRID
                     VMATAVWNEMPVDEIAQLDLAYAPPFSPIWDPVIIAAQQALKQL"
BASE COUNT          564 a         1177 c         1162 g          564 t
ORIGIN      
        1 cgacaccact cgcgctgcgt actcgcgctt gcacagcgtg ccgcgcggga agtcccacag
       61 cggccgctcg ccgcgctgcg gcttgtacac gacgagcatc tgcgcgtcac cggtgaccgt
      121 cgccagatac gtcgcgttcg acgaccacgg catccgcccg aggatgtcga tctcaccctc
      181 gcgcaacaac gtgtgcgcga gatcgtcggg gacggccgct tgcggcggcc agccggtctc
      241 cgccacgccg cgacgctaat tccaacgcgg gcagatgtgc ccgtcggggt ccatcgggaa
      301 gtcgcacagg tcgcaacggg gccggccggc cgacaccgcc tgggcaccga cctcgaccat
      361 ggctcgcacc tgtgcgcgcg tcgcgtacag ccgcgcgatg cggccttcgc tgtcttccag
      421 cgcgggcggg gcttcttcct cttcggttgc ggtctcgcgc aactcgatga ggacgagatg
      481 gcgttcgggg tcgtagccga tgccgatcaa acgggcgcgg aacagcggtt cgtcctcgat
      541 cgggggatcg gagacgacat cgtcgtcatc gtcgtcgtcg ggctcgtctt cgtcgatgcg
      601 atcgaggaat tgtccagctt cggtggcgag gagcgcgacc tgttcctttt cgacgagcac
      661 cgacagcaac gccgaaccct tacgcgcctg aatgacgaag gtgcgcgcgc cgggctggcc
      721 gaacgcgccg gcaacgatgc cgtcaaccgg atcgaactcg atgacgtcgg ccacggtgcg
      781 tcactcctcc gtgggaagca ggtcttcgag gccgcccgtg tcgttgcatt tgaccatcat
      841 cgaaccgaac gggtggaagt cgaacacggt caccgacgcc ggcgagacat gtacccgctg
      901 gaagagatcg agatgcatgc ccgtgtagtg ggcgatcgcg gacttgatcg gatcggcgtg
      961 actcaccacg acgaacgttt cgtgcgggtg cgcggcgacg acttcttcca acgcctcgac
     1021 catgcgcgcc tgcatggcac gtatggtctc gccaccgggg aagcgcgcgc gcgacggtgc
     1081 gacctgtacg accttccact cttcggtctt ggcgaggtcg gcgatcttgc cgccggtcca
     1141 ctccccgtag tcggcttcga tgacaccggg cagcggctgc acgtcgagac catggcgggc
     1201 ggcgatctct tcggcggtct gcgtcgtgcg ttcgatcggg ctcgcgtaca cggcggcaac
     1261 cggaagcttc gcgagccggt cggcggtcgc ggccgcctgc ccgcgaccct tctcggacag
     1321 gtcgatgccc ggcatgcgcc ccgacagcag cggtcccgtg tgcgcggtca cggcatggcg
     1381 cacgagcacc agccgcgtcg gcggcttcgg cttctctttc gcgccgttct tctcgtcgtt
     1441 cgtctcggcg gtgtcgaccg cctcggcccc agtcacggtc gaccacgata gttgtgaccg
     1501 gtcgccatac gatcctcgcc atgactgatg tgcacctgtc cgcctcgggg ccgcccgaga
     1561 cggtgctcga tccgcccgac gacgacgccg cggatgcgct gcgtgccgcg ctggcgcagc
     1621 cgatcgaagg gcgccgggac gcggtcgcgg acgtgtgcgc gcgctggccg cgctacatcg
     1681 acgcatgggc gcgactcggg tcgctcgcgc gcgacgacgt cgaggcctac gcctgctacc
     1741 gcgtcggcta ccaccgcgga ctcgatcagc tgcgcgcctc gggatggcgc ggcagtggat
     1801 acgtacgctg gcggcacgag acgaaccgtg gcttcctgcg cgcgctcgac ggcctgcgag
     1861 cggcagccga ggcgatcggc gaggccgacg aagccgaccg ctgccggatg ttcttgcacc
     1921 agctggaccc ggattgggac cggaggaacg agtagcgttt catcccatga aacgagcgct
     1981 gatcacgggc atcaccggcc aggacgggcg acatctgtcg caactgctgg cgacgaaggg
     2041 ataccaagtc ttcgggctcg tacacgggca ggcgaacccg aagatcgacc tcgtgcaggg
     2101 tgagaacccg tcgctcgagt tggtcgaggg cgacctccgc gacctctcgt cgctcatcgg
     2161 agtcgtcgag caggtgcaac cggacgaggt ctacaacctc ggcgcgatca gcttcgtgca
     2221 gctctcgttc aagcaggccg agctgacggc ggagatcacc gggctcggcg tgttgcgcat
     2281 gctcgaagcg gtgcgcatcg tcggcggtga gaacaacccg atccgtttct accaggcgtc
     2341 gtcgtcggag atgttcggca aggtgcgcga gacgccgcag accgaagaga cgcccttcca
     2401 cccgcgttcg ccgtacggcg cggccaaggt gttcggccac tacacgacgg tcaactatcg
     2461 cgaggcgtac aacatccacg cgagcagtgg catcctcttc aaccacgagg gcccgcgccg
     2521 cggcctcgag ttcgtgacgc gcaaggtcac caactcggtc gcgcgcatca agctcggcct
     2581 gcaggacagc atcagcctcg gcaacctcga ctccaagcgc gactggggct acgcgggcga
     2641 ctacgtgcag gcaatgtgga tgatgctcca acaagacgag cccgacgact acgtcatcgc
     2701 gaccggcgag acgcactcga tccgcgactt cctcgatgcc gcgttcgagc tcgccgagat
     2761 cgacgactgg gcgcgctacg tcaagcagga cccgcgtttc taccgtccga cggaggtcga
     2821 cctcctcatc ggtgacgcgt cgaaggcgaa gaagaagctg ggctgggagc cgtcggtcag
     2881 cttccccgag ctcgtgcaga tgatgtacga gaacgacctc aaggaagaga gcgagaaagc
     2941 cgcccgttag accgcgcgcg tcagggtggg aagcggaccg gaggccaccg ccgaaggccg
     3001 cgctccggag ggagcgggcg ggaagatcag agctgcttca gcgcctgctg ggcggcgatg
     3061 atcacggggt cccagatcgg cgagaacggc ggcgcatagg ccaggtcgag ctgcgcgatc
     3121 tcgtcgacgg gcatctcgtt ccacaccgcg gtcgccatca cgtcgatgcg tttggccgcg
     3181 ccctcccggc cgatgatctg cgcgccgagc agccgcccgc tcgacttctc ggccagcacc
     3241 ttcacggtga tcttcgacgc gcccgggtag tagccggcac gcgtcgtgct ctcgatcttc
     3301 gctgtcacgt agtcgaagcc ggcgtccttg gcttcgtgct ccgtgagtcc ggtgcgaccg
     3361 atctcgacgt tgcagatgcg tgtcactgcg gtcccgacga caccgccgaa cgacgcatgg
     3421 cctcccgtcg cgttcacacc cgcgacccgg ccctgcttgt tcgcgtg
//
